title: Development of the OPgun for bombardment of animal tissues.
abstract: A simple and inexpensive particle-bombardment device, the OPgun, was constructed for the delivery of DNA into animal tissues. This device is based on the particle-inflow gun first described for plant-cell transfection. The delivery of tungsten particles into the epidermis of the mouse ear, without the use of vacuum and without causing damage to the tissue, was demonstrated. The system was also shown to be capable of inducing antibodies to a foreign gene in mice.
authors.affiliation: Onderstepoort Veterinary Institute, South Africa.
authors.forename: K A
authors.initials: KA
authors.lastname: Brayton
authors.forename: G C
authors.initials: GC
authors.lastname: Bothma
authors.forename: S W
authors.initials: SW
authors.lastname: Vogel
authors.forename: B A
authors.initials: BA
authors.lastname: Allsopp
mesh_terms: D000818:Animals; D000906:Antibodies / Q000097:blood; D004251:DNA Transposable Elements; D004797:Enzyme-Linked Immunosorbent Assay; D004867:Equipment Design; D005260:Female; D005818:Genetic Engineering / Q000295:instrumentation*; D007277:Injections, Jet / Q000295:instrumentation*; D051379:Mice; D010957:Plasmids / Q000008:administration & dosage
chemical_list: D000906:Antibodies; D004251:DNA Transposable Elements