Capillary zone electrophoresis separation of tryptophan and its metabolites, including quinolinic acid.
Capillary zone electrophoresis with UV absorbance detection was used to separate tryptophan and ten of its metabolites. Run buffers of pH 4.0-10.0 were evaluated for their effect on resolution; a pH 9.6 buffer was found to give optimum separation of all components. Ethylenediaminetetraacetic acid (EDTA), which prevents complexation of some analytes with polyvalent cations, was included in the run buffer to insure good peak shape and reproducible mobilities. The resulting method was used to detect the presence of quinolinic acid in a urine sample.
Weber
P L
PL
Department of Chemistry, Briar Cliff College, Sioux City, IA 51104, USA.
Malis
M
M
Palmer
S D
SD
Klein
T L
TL
Lunte
S M
SM
D002021:Buffers; D004492:Edetic Acid; D019075:Electrophoresis, Capillary; D006801:Humans; D006863:Hydrogen-Ion Concentration; D017378:Quinolinic Acid / Q000652:urine*; D015203:Reproducibility of Results; D013056:Spectrophotometry, Ultraviolet; D014364:Tryptophan / Q000302:isolation & purification* / Q000378:metabolism
D002021:Buffers; D014364:Tryptophan; D004492:Edetic Acid; D017378:Quinolinic Acid