Cross-clade human immunodeficiency virus (HIV)-specific cytotoxic T-lymphocyte responses in HIV-infected Zambians.
We have examined cross-clade HIV-specific cytotoxic T-lymphocyte (CTL) activity in peripheral blood of eight Zambian individuals infected with non-B-clade human immunodeficiency virus type 1 (HIV-1). Heteroduplex mobility assay and partial sequence analysis of env and gag genes strongly suggests that all the HIV-infected subjects were infected with clade C HIV-1. Six of eight C-clade HIV-infected individuals elicited CTL activity specific for recombinant vaccinia virus-infected autologous targets expressing HIV gag-pol-env derived from B-clade HIV-1 (IIIB). Recognition of individual recombinant HIV-1 B-clade vaccinia virus-infected targets expressing gag, pol, or env was variable among the patients tested, indicating that cross-clade CTL activity is not limited to a single HIV protein. These data demonstrate that HIV clade C-infected individuals can mount vigorous HIV clade B-reactive CTL responses.
Betts
M R
MR
Department of Microbiology and Immunology, University of North Carolina at Chapel Hill, 27599-7290, USA.
Krowka
J
J
Santamaria
C
C
Balsamo
K
K
Gao
F
F
Mulundu
G
G
Luo
C
C
N'Gandu
N
N
Sheppard
H
H
Hahn
B H
BH
Allen
S
S
Frelinger
J A
JA
D003429:Cross Reactions; D004279:DNA, Viral / Q000235:genetics; D015488:HIV Antigens / Q000276:immunology*; D015658:HIV Infections / Q000276:immunology* / Q000821:virology; D015497:HIV-1 / Q000276:immunology*; D006801:Humans; D007111:Immunity, Cellular; D010802:Phylogeny; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology*; D014481:United States; D015024:Zambia
D004279:DNA, Viral; D015488:HIV Antigens