title: Replication of murine coronaviruses in mouse embryonic stem cell lines in vitro.
abstract: Replication of murine coronaviruses in eight mouse embryonic stem (ES) cell lines of several genetic backgrounds was examined. Both mouse hepatitis virus (MHV) type 2 and MHV, strain A59 replicated well with no or minimal cytopathic effect in all the ES cell lines tested. The results suggest the possibility that MHV-infected ES cells may disseminate MHV in mouse colonies due to embryo manipulation.
authors.affiliation: Department of Animal Pathology, University of Tokyo, Japan.
authors.forename: S
authors.initials: S
authors.lastname: Kyuwa
mesh_terms: D000818:Animals; D002460:Cell Line; D018562:Disease Transmission, Infectious; D004622:Embryo, Mammalian / Q000821:virology; D005818:Genetic Engineering; D066298:In Vitro Techniques; D051379:Mice; D006517:Murine hepatitis virus / Q000502:physiology*; D013234:Stem Cells / Q000821:virology; D014779:Virus Replication