Speed congenics: a classic technique in the fast lane (relatively speaking).
Marker-assisted selection protocol (MASP)-based strategies produce congenic strains with the target gene contained on clearly defined donor-derived genomic intervals in less than half the member of generations required by the classic protocol. Thus, the quality and speed of congenic strain construction are enhanced by this methodology. Here, Edward Wakeland and colleagues compare various MASP-based strategies and discuss their advantages with reference to immunological traits.
Wakeland
E
E
Center for Mammalian Genetics, College of Medicine, University of Florida, Gainesville 32610-0275, USA. wakeland@cmg.health.ufl.edu
Morel
L
L
Achey
K
K
Yui
M
M
Longmate
J
J
D000818:Animals; D003198:Computer Simulation; D003433:Crosses, Genetic; D005260:Female; D005819:Genetic Markers; D005821:Genetic Techniques; D007125:Immunogenetics; D008297:Male; D051379:Mice; D008822:Mice, Transgenic
D005819:Genetic Markers