Isolation of the transposable element hupfer from the entomopathogenic fungus Beauveria bassiana by insertion mutagenesis of the nitrate reductase structural gene.
A transposable element has been isolated from the entomopathogenic fungus Beauveria bassiana by trapping it in the nitrate reductase structural gene, which has been cloned from this species. The element had inserted in the first exon of the nia gene and appeared to have duplicated the sequence TA at the site of insertion. It was 3336 bp long with 30-bp imperfect, inverted, terminal repeats. The element, called hupfer, contained an open reading frame encoding a 321-amino acid protein similar to the IS630- or mariner-Tcl-like transposases, and a residual sequence of about 2 kb which was not significantly similar to any published sequence. There are fewer than five copies of this transposable element present per genome in the fungus.
Maurer
P
P
I.N.R.A., Station de Recherches de Lutte Biologique, La Minière, Guyancourt, France. maurer@versailles.inra.fr
Réjasse
A
A
Capy
P
P
Langin
T
T
Riba
G
G
D000595:Amino Acid Sequence; D001483:Base Sequence; D003001:Cloning, Molecular; D015342:DNA Probes / Q000235:genetics; D004251:DNA Transposable Elements / Q000235:genetics*; D004271:DNA, Fungal / Q000235:genetics; D004268:DNA-Binding Proteins / Q000235:genetics; D010450:Endopeptidases; D005091:Exons; D005656:Fungal Proteins / Q000235:genetics*; D003904:Mitosporic Fungi / Q000235:genetics*; D008969:Molecular Sequence Data; D016254:Mutagenesis, Insertional; D050901:Nitrate Reductase; D009565:Nitrate Reductases / Q000235:genetics*; D009713:Nucleotidyltransferases / Q000235:genetics; D016366:Open Reading Frames; D016133:Polymerase Chain Reaction; D012091:Repetitive Sequences, Nucleic Acid; D015183:Restriction Mapping; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D019895:Transposases; D014764:Viral Proteins
D015342:DNA Probes; D004251:DNA Transposable Elements; D004271:DNA, Fungal; D004268:DNA-Binding Proteins; D005656:Fungal Proteins; D014764:Viral Proteins; C108868:hupfer protein, Beauveria bassiana; D009565:Nitrate Reductases; D050901:Nitrate Reductase; D009713:Nucleotidyltransferases; C081454:Tc1 transposase; D019895:Transposases; D010450:Endopeptidases; C059992:nuclear inclusion protein a, mosaic viruses