title: A scanning force- and fluorescence light microscopy study of the structure and function of a model pulmonary surfactant.
abstract: The structure of an artificial pulmonary surfactant was studied by scanning force- and fluorescence light microscopy (SFM, and FLM, respectively). The surfactant--a mixture of dipalmitoylphosphatidylcholine (DPPC), dipalmitoylphosphatidylglycerol (DPPG) and recombinant surfactant-associated protein C (SP-C)--was prepared at the air-water interface of a Langmuir film balance and imaged by FLM under various states of compression. In order to visualize their topography by SFM, the films were transferred onto a solid mica support by the Langmuir-Blodgett (LB) technique. We found that a region of high film compressibility of the spread monolayer close to its equilibrium surface pressure (pi = 50 mN/m) was due to the exclusion of layered protrusions with each layer 5.5 to 6.5 nm thick. They remained associated with the monolayer and readily reinserted upon expansion of the film. Comparison with the FLM showed that the protrusions contained the protein in high concentration. The more the film was compressed, the larger was the number of layers on top of each other. The protrusions arose from regions of the monolayer with a distinct microstructure that may have been responsible for their formation. The molecular architecture of the microstructure remains to be elucidated, although some of it can be inferred from spectroscopic data in combination with the SFM topographical images. We illustrate our current understanding of the film structure with a molecular model.
authors.affiliation: Institut für Medizinische Physik und Biophysik, Westfälische Wilhelms-Universität, Münster, Germany.
authors.forename: M
authors.initials: M
authors.lastname: Amrein
authors.forename: A
authors.initials: A
authors.lastname: von Nahmen
authors.forename: M
authors.initials: M
authors.lastname: Sieber
mesh_terms: D015060:1,2-Dipalmitoylphosphatidylcholine / Q000737:chemistry*; D000595:Amino Acid Sequence; D006801:Humans; D018625:Microscopy, Atomic Force; D008856:Microscopy, Fluorescence; D016252:Microscopy, Scanning Tunneling; D008969:Molecular Sequence Data; D010715:Phosphatidylglycerols / Q000737:chemistry*; D011510:Proteolipids / Q000737:chemistry* / Q000502:physiology*; D011663:Pulmonary Surfactants / Q000737:chemistry* / Q000502:physiology*; D013329:Structure-Activity Relationship
chemical_list: D010715:Phosphatidylglycerols; D011510:Proteolipids; D011663:Pulmonary Surfactants; D015060:1,2-Dipalmitoylphosphatidylcholine; C030345:1,2-dipalmitoylphosphatidylglycerol