[The photometric determination of the antilysozyme activity of microorganisms].
The method for the determination of the antilysozyme activity (ALA) of microorganisms, based on the photometric determination of the residual activity of enzyme with the use of Micrococcus lysodeikticus test culture after the incubation of the strain under test and lysozyme, is proposed. The new method enhances the reliability of the determination of the ALA of microorganisms due to an increase in the accuracy of the quantitative determination of ALA. The elimination of the stage of interaction between the growing bacterial culture and lysozyme and the presence of the antilysozyme factor in the supernatant fluid confirm the constructive and secretory character of antilysozyme activity.
Bukharin
O V
OV
Valyshev
A V
AV
Elagina
N N
NN
Ivanov
Iu B
IuB
Cherkasov
S V
SV
D001419:Bacteria / Q000276:immunology*; D001431:Bacteriological Techniques / Q000706:statistics & numerical data; D013293:Enterococcus faecalis / Q000276:immunology; D004926:Escherichia coli / Q000276:immunology; D008837:Micrococcus / Q000187:drug effects; D009113:Muramidase / Q000037:antagonists & inhibitors* / Q000494:pharmacology; D010783:Photometry / Q000379:methods* / Q000706:statistics & numerical data; D011513:Proteus mirabilis / Q000276:immunology; D013211:Staphylococcus aureus / Q000276:immunology; D013212:Staphylococcus epidermidis / Q000276:immunology
D009113:Muramidase