Expression of drug-metabolizing enzymes.
A principal advance in the production of drug-metabolizing enzymes has been the development of catalytically self-sufficient cytochrome P450 systems, including additional P450-reductase fusion proteins and Escherichia coli and baculovirus coexpression constructs. Continuing work with glutathione transferases has resulted in the identification of important residues by random mutagenesis screening techniques, as well as in the engineering of model Salmonella typhimurium strains for genotoxicity analysis.
Guengerich
F P
FP
Department of Biochemistry, Vanderbilt University School of Medicine, Nashville, TN 37232, USA. guengerich@toxicology.mc.vanderbilt.edu
Parikh
A
A
D000818:Animals; D002460:Cell Line; D003001:Cloning, Molecular / Q000379:methods*; D003577:Cytochrome P-450 Enzyme System / Q000096:biosynthesis*; D004926:Escherichia coli; D005818:Genetic Engineering / Q000379:methods; D016296:Mutagenesis; D009152:Mutagenicity Tests; D009251:NADPH-Ferrihemoprotein Reductase / Q000096:biosynthesis*; D004364:Pharmaceutical Preparations / Q000378:metabolism; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis*; D011994:Recombinant Proteins / Q000096:biosynthesis*; D012486:Salmonella typhimurium; D014162:Transfection / Q000379:methods*
D004364:Pharmaceutical Preparations; D011993:Recombinant Fusion Proteins; D011994:Recombinant Proteins; D003577:Cytochrome P-450 Enzyme System; D009251:NADPH-Ferrihemoprotein Reductase