Characterization of several pseudorabies viral strains by virus-neutralization test using monoclonal antibodies.
In the present study, five mouse monoclonal antibodies (MAbs) to the pseudorabies virus (PRV) Yamagata-81 strain were produced. The MAbs were used in cross-neutralization tests and cross-indirect enzyme-linked immunosorbent assay (ELISA) against three PRV viral strains isolated in Argentina and another four obtained from the United States, Japan, France, and Sweden. Four of five MAbs needed the presence of complement to produce or enhance neutralization activity. No differences were observed by ELISA. The MAbs showed different neutralizing activity against PRV strains, suggesting phenotypic heterogeneity among them.
Nosetto
E O
EO
Laboratory of Virology, Faculty of Veterinary Sciences, National University of La Plata, Argentina.
Echeverria
M G
MG
Horimoto
T
T
Pecoraro
M R
MR
Galosi
C M
CM
Tohya
Y
Y
Norimine
J
J
Takahashi
E
E
Etcheverrigaray
M E
ME
Mikami
T
T
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D000914:Antibodies, Viral / Q000276:immunology*; D000956:Antigens, Viral / Q000276:immunology; D002417:Cattle; D002460:Cell Line; D004797:Enzyme-Linked Immunosorbent Assay; D011558:Herpesvirus 1, Suid / Q000145:classification* / Q000276:immunology / Q000302:isolation & purification; D051379:Mice; D009500:Neutralization Tests; D014759:Viral Envelope Proteins / Q000276:immunology
D000911:Antibodies, Monoclonal; D000914:Antibodies, Viral; D000956:Antigens, Viral; D014759:Viral Envelope Proteins; C107752:glycoprotein D, pseudorabies virus; C070915:glycoprotein gII, pseudorabies virus