ELISA for detection of dengue virus-induced cytokine and its antibody.
ELISA technique has been standardized for detection of a dengue type 2 virus (DV)-induced cytokine, the cytotoxic factor (CF) and CF-specific antibodies. The performed ELISA was found to be sensitive (90.9%), specific (92.5%), accurate (91%) and reproducible and was able to detect a minimum of 7 ng/ml CF in the test samples. The technique was used to detect CF in DV inoculated mouse sera and DV-infected mouse spleen cell culture fluids. Significant utility of the test was detection of a CF-like cytokine in the sera of human cases of dengue haemorrhagic fever.
Mukerjee
R
R
Postgraduate Department of Microbiology, K G Medical College, Lucknow, India.
Chaturvedi
U C
UC
D000818:Animals; D000914:Antibodies, Viral / Q000096:biosynthesis*; D016207:Cytokines / Q000096:biosynthesis*; D003716:Dengue Virus / Q000502:physiology*; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D051379:Mice
D000914:Antibodies, Viral; D016207:Cytokines