Comparison of charcoal- and starch-based media for testing susceptibilities of Legionella species to macrolides, azalides, and fluoroquinolones.
We compared growth characteristics of 46 Legionella strains grown on buffered charcoal yeast extract alpha (BCYE alpha) agar and buffered starch yeast extract (BSYE) agar and MICs of macrolides, azalides, and fluoroquinolones for these organisms. Growth was poor and not reproducible on BSYE agar. Growth was excellent on BCYE alpha, and MICs were easy to interpret. BCYE alpha is superior to BSYE for testing susceptibilities of Legionella species by agar dilution.
Pendland
S L
SL
Department of Pharmacy Practice, University of Illinois, Chicago 60612, USA.
Martin
S J
SJ
Chen
C
C
Schreckenberger
P C
PC
Danziger
L H
LH
D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D000890:Anti-Infective Agents / Q000494:pharmacology*; D017963:Azithromycin / Q000494:pharmacology; D002606:Charcoal; D002939:Ciprofloxacin / Q000494:pharmacology; D017291:Clarithromycin / Q000031:analogs & derivatives / Q000494:pharmacology; D003470:Culture Media; D004917:Erythromycin / Q000494:pharmacology; D006801:Humans; D007875:Legionella / Q000145:classification / Q000187:drug effects* / Q000254:growth & development; D016952:Legionella pneumophila / Q000145:classification / Q000187:drug effects / Q000254:growth & development; D007876:Legionellosis / Q000382:microbiology*; D008826:Microbial Sensitivity Tests / Q000379:methods*; D015242:Ofloxacin / Q000494:pharmacology; D015203:Reproducibility of Results; D013213:Starch
D000900:Anti-Bacterial Agents; D000890:Anti-Infective Agents; D003470:Culture Media; D002606:Charcoal; D002939:Ciprofloxacin; D004917:Erythromycin; D017963:Azithromycin; D013213:Starch; D015242:Ofloxacin; D017291:Clarithromycin