title: Effects of hemodilution on splanchnic perfusion and hepatorenal function. II. Renal perfusion and hepatorenal function.
abstract: Hepatorenal perfusion and function were assxssed in 22 dogs undergoing acute normovolemic hemodilution (ANH) to a hematocrit (Hct) of 20% using 6% hydroxyethyl starch (200.000/0.5) as the diluent. Organ perfusion was determined with the radioactive microspheres method. Renal function was assessed by urinary output, creatinine clearance and fractional sodium excretion. Blood volume as well as hepatic function were derived from indocyanine green (ICG) dilution kinetics. Hepatocellular integrity was determined by serum enzymatic activity of glutamate-oxalacetate-transaminase (GOT) and glutamate-pyruvate- transaminase (GPT). ANH to Hct 20% did not change blood volume and mean aortic pressure, while heart rate was slightly elevated (p<0.05) by 5 beats per minute and cardiac output increased by 29% (p<0.05). In contrast to the liver, where arterial and portal venous blood flow increased (86% and 28%, respectively; p<0.05), total renal blood flow as well as intraorgan distribution of renal blood flow remained unchanged post-ANH. While creatinine clearance remained unchanged following ANH, urinary output and fractional urinary excretion increased (p<0.05). In response to enhanced hepatic blood flow after ANH, intravascular half-life of ICG was reduced (p<0.05) and ICG clearance increased (p<0.05). Serum enzymatic activity of GPT decreased upon ANH (p<0.05), while GOT activity remained unchanged. ANH to a Hct 20% does not impair hepatorenal function. Increased urinary output points out the necessity for proper adjustment of crystalloid infusion to maintain normal intravascular volume and avoid hypovolemia and the associated risk of tissue hypoxia.
authors.affiliation: Institute for Surgical Research and Institute of Anesthesiology Marchioninistr. 15 Munich, D-81366 Germany +49 89/7095 4357 +49 89/7095 8897 habler@icf.med.uni-muenchen.de.
authors.forename: O
authors.initials: O
authors.lastname: Habler
authors.forename: M
authors.initials: M
authors.lastname: Kleen
authors.forename: J
authors.initials: J
authors.lastname: Hutter
authors.forename: A
authors.initials: A
authors.lastname: Podtschaske
authors.forename: M
authors.initials: M
authors.lastname: Tiede
authors.forename: G
authors.initials: G
authors.lastname: Kemming
authors.forename: C
authors.initials: C
authors.lastname: Corso
authors.forename: S
authors.initials: S
authors.lastname: Batra
authors.forename: P
authors.initials: P
authors.lastname: Keipert
authors.forename: S
authors.initials: S
authors.lastname: Faithfull
authors.forename: K
authors.initials: K
authors.lastname: Messmer
mesh_terms: D000818:Animals; D001810:Blood Volume; D004231:Diuresis; D004285:Dogs; D005260:Female; D006438:Hemodilution / Q000009:adverse effects; D006439:Hemodynamics; D007668:Kidney / Q000502:physiology*; D008099:Liver / Q000502:physiology*; D008297:Male; D009318:Natriuresis; D012039:Regional Blood Flow; D012079:Renal Circulation / Q000502:physiology*; D013152:Splanchnic Circulation / Q000502:physiology*