title: Peripheral eosinophil count both before and after liver transplantation predicts acute cellular rejection.
abstract: Acute cellular rejection is common after orthotopic liver transplantation and an important cause of graft dysfunction. Eosinophils, potent mediators of tissue damage, have been implicated in the pathogenesis of acute rejection. We studied 55 patients, all of whom had a protocol biopsy 7 days after transplantation and whose peripheral eosinophil count was monitored daily for 11 days after transplantation. Patients were divided clinicopathologically into two groups: group A, without rejection, group B, with rejection. Group B (36% of patients) developed rejection within the 11-day study period. The pretransplant eosinophil count was significantly higher in group B, compared with group A (0.31 +/- 0.08 v 0.10 +/- 0.01 (x10(9)/L), p < .001). After transplantation, the eosinophil count fell to low levels in both groups. By day 3 there was a statistically significant rise in the eosinophil count in group B compared with group A, with a maximum at day 7 [0.51 +/- 0.06 v 0.26 +/- 0.03 (x10(9)/L) p < .001]. After treatment with steroids, the eosinophil count dropped to values similar to those in group A and remained low thereafter in 16 of 20 patients. Four patients had a second episode of rejection; in each of these, eosinophils were raised again and decreased with resolution of the rejection. An eosinophil count threshold of 0.13 (x10(9)/L) before transplantation and 0.33 (x10(9)/L) on day 7 after transplantation predicted the development of rejection (sensitivity 72/70%, specificity 66/63%, negative predictive value 82/79%). We conclude that a raised eosinophil count is associated with acute rejection. The raised eosinophil count before transplantation in group B suggests that these patients are predisposed to acute rejection, and earlier intervention may be indicated.
authors.affiliation: Department of Medicine, Royal Infirmary, University of Edinburgh, UK. mdoll@srv2.med.ed.ac.uk
authors.forename: M M
authors.initials: MM
authors.lastname: Dollinger
authors.forename: J N
authors.initials: JN
authors.lastname: Plevris
authors.forename: I A
authors.initials: IA
authors.lastname: Bouchier
authors.forename: D J
authors.initials: DJ
authors.lastname: Harrison
authors.forename: P C
authors.initials: PC
authors.lastname: Hayes
mesh_terms: D000328:Adult; D004804:Eosinophils / Q000166:cytology*; D005260:Female; D005544:Forecasting; D006084:Graft Rejection / Q000097:blood*; D006801:Humans; D007958:Leukocyte Count; D016031:Liver Transplantation / Q000009:adverse effects*; D008297:Male; D008875:Middle Aged; D011379:Prognosis; D012189:Retrospective Studies; D013997:Time Factors