title: Effect of cytochalasin D on systemic and local anaphylaxis in a murine model.
abstract: We investigated the effect of cytochalasin D on anaphylaxis. Cytochalasin D dose-dependently inhibited systemic anaphylaxis induced by compound 48/80 in rats. Especially, cytochalasin D inhibited compound 48/80-induced systemic anaphylaxis 100% with a dose of 1 microg g-1 body weight (BW). Cytochalasin D significantly inhibited serum histamine levels induced by compound 48/80. Cytochalasin D (10(-1) microg g-1 BW) also inhibited local anaphylaxis activated by anti-dinitrophenyl (DNP) IgE to 79.6+/-1.8%. Cytochalasin D dose-dependently inhibited histamine release from the rat peritoneal mast cells activated by compound 48/80 or anti-DNP IgE. The level of cAMP in rat peritoneal mast cells, when cytochalasin D was added, transiently and significantly increased about fourfold compared with that of basal cells. Our studies provide evidence that cytochalasin D will be beneficial in the treatment of anaphylaxis.
authors.affiliation: College of Pharmacy, Wonkwang University, Iksan, South Korea.
authors.forename: H Y
authors.initials: HY
authors.lastname: Shin
authors.forename: E H
authors.initials: EH
authors.lastname: Lee
authors.forename: T Y
authors.initials: TY
authors.lastname: Shin
authors.forename: H M
authors.initials: HM
authors.lastname: Kim
mesh_terms: D000707:Anaphylaxis / Q000139:chemically induced / Q000517:prevention & control*; D000818:Animals; D018927:Anti-Asthmatic Agents / Q000627:therapeutic use; D004205:Cromolyn Sodium / Q000627:therapeutic use; D000242:Cyclic AMP / Q000378:metabolism*; D015638:Cytochalasin D / Q000627:therapeutic use*; D006636:Histamine Release / Q000187:drug effects*; D007073:Immunoglobulin E; D008407:Mast Cells / Q000187:drug effects* / Q000378:metabolism; D019384:Nucleic Acid Synthesis Inhibitors / Q000627:therapeutic use*; D010323:Passive Cutaneous Anaphylaxis / Q000187:drug effects; D051381:Rats; D017208:Rats, Wistar; D003189:p-Methoxy-N-methylphenethylamine
chemical_list: D018927:Anti-Asthmatic Agents; D019384:Nucleic Acid Synthesis Inhibitors; D015638:Cytochalasin D; D007073:Immunoglobulin E; D003189:p-Methoxy-N-methylphenethylamine; D000242:Cyclic AMP; D004205:Cromolyn Sodium