The effect of sarcoplasmic reticulum blockade on the force/frequency relationship and systolic contraction patterns in the newborn pig heart.
We investigated the relationship between heart rate and contractility in seven anaesthetized young piglets by measuring contractility at different atrial pacing rates. To study the origin of this relationship we repeated the measurements after blocking the sarcoplasmic reticulum calcium release channel with ryanodine. We assessed contractility using indices derived from instantaneous left ventricular pressure and volume measured by micromanometric and conductance catheters during rapid inferior vena cava occlusion, thus generating the end-systolic pressure-volume relationship, which was characterized by its slope Ees, and the maximum rate of change of ventricular pressure (dP/dtmax)/end-diastolic volume relationship, also characterized by its slope. All animals showed an increase in contractility with increasing heart rate (intact force/frequency relationship) which was abolished after ryanodine. The most striking effect of ryanodine on baseline haemodynamics was the dramatic decrease of dP/dtmax to about 50% of its original value, while peak developed pressure and Ees did not change. We conclude that the young piglet, despite its immaturity, has a functional sarcoplasmic reticulum, illustrated by an intact force/frequency relationship. In addition, blockade of the sarcoplasmic reticulum in vivo has profoundly different effects during early and late systole, indicating that indices of contractility derived during different parts of the cardiac cycle represent different aspects of systole.
Klautz
R J
RJ
University Hospital Rotterdam/Dijkzigt, Department of CardioThoracic Surgery, Thoraxcentre, Room Bd 156, Dr Molewaterplein 40, 3015 GD Rotterdam, The Netherlands.
Baan
J
J
Teitel
D F
DF
D000818:Animals; D000831:Animals, Newborn / Q000502:physiology*; D006339:Heart Rate / Q000187:drug effects; D006439:Hemodynamics / Q000187:drug effects; D009200:Myocardial Contraction / Q000187:drug effects*; D012433:Ryanodine / Q000494:pharmacology*; D019837:Ryanodine Receptor Calcium Release Channel / Q000187:drug effects / Q000502:physiology; D012519:Sarcoplasmic Reticulum / Q000187:drug effects* / Q000502:physiology*; D013552:Swine; D013599:Systole / Q000502:physiology; D017725:Ventricular Pressure / Q000187:drug effects
D019837:Ryanodine Receptor Calcium Release Channel; D012433:Ryanodine