Transgenic pigs produce functional human factor VIII in milk.
Deficiency or abnormality of coagulation factor VIII (FVIII) causes a bleeding disorder called hemophilia A. Treatment involves FVIII concentrates prepared from pooled human plasma or recombinant FVIII (rFVIII) prepared from mammalian cell culture. The cost of highly purified FVIII or rFVIII is a major factor in hemophilia therapy and restricts prophylaxis. We have sought to generate a new source of rFVIII by targeting expression of the human FVIII cDNA to the mammary gland of transgenic pigs using the regulatory sequences of the mouse whey acidic protein gene. The identity of processed heterodimeric rFVIII was confirmed using specific antibodies, by thrombin digestion and activity assays. The secretion of as much as 2.7 micrograms/ml of rFVIII in milk was over tenfold higher than in normal plasma. Up to 0.62 U/ml of rFVIII was detected in an assay in which rFVIII restored normal clotting activity to FVIII-deficient human plasma.
Paleyanda
R K
RK
Plasma Derivatives Department Holland Laboratory, Rockville, MD 20855, USA.
Velander
W H
WH
Lee
T K
TK
Scandella
D H
DH
Gwazdauskas
F C
FC
Knight
J W
JW
Hoyer
L W
LW
Drohan
W N
WN
Lubon
H
H
D000818:Animals; D030801:Animals, Genetically Modified; D001777:Blood Coagulation / Q000187:drug effects; D018076:DNA, Complementary / Q000096:biosynthesis*; D019281:Dimerization; D005169:Factor VIII / Q000096:biosynthesis* / Q000235:genetics / Q000494:pharmacology; D005260:Female; D005786:Gene Expression Regulation / Q000235:genetics; D006467:Hemophilia A / Q000188:drug therapy / Q000191:economics; D006801:Humans; D008321:Mammary Glands, Animal / Q000378:metabolism*; D051379:Mice; D008892:Milk / Q000737:chemistry*; D008894:Milk Proteins / Q000235:genetics; D011994:Recombinant Proteins / Q000096:biosynthesis; D013552:Swine / Q000235:genetics*; D013917:Thrombin
D018076:DNA, Complementary; D008894:Milk Proteins; D011994:Recombinant Proteins; C024471:whey acidic proteins; D005169:Factor VIII; D013917:Thrombin