The effect of interleukin-10 on meningeal inflammation in experimental bacterial meningitis.
Interleukin-10 (IL-10) is a cytokine with antiinflammatory effects. In a rabbit model of meningitis, IL-10 was given intracisternally or intravenously to evaluate the impact on inflammation induced by lipooligosaccharide (LOS), Haemophilus influenzae type b (Hib), or Listeria monocytogenes. Intracisternal IL-10 in concentrations >1 microg significantly reduced tumor necrosis factor-alpha (TNF-alpha) and lactate values in cerebrospinal fluid (CSF). Intravenous IL-10 (1 mg/kg) in two doses after intracisternal LOS significantly reduced CSF TNF-alpha and lactate. When Hib was used, animals were treated with ceftriaxone and dexamethasone with or without IL-10 (1 mg/kg). TNF-alpha was significantly reduced in animals treated with IL-10, dexamethasone, or both compared with levels in rabbits receiving ceftriaxone alone. Comparable results were obtained when L. monocytogenes was inoculated and animals were treated with ampicillin with or without IL-10, dexamethasone, or nothing. In conclusion, IL-10 modulates CSF TNF-alpha concentrations in experimental LOS, Hib, or L. monocytogenes meningitis. The maximal inhibitory effect was seen when IL-10 and dexamethasone were combined.
París
M M
MM
Department of Pediatrics, University of Texas Southwestern Medical Center at Dallas, 75235-9063, USA.
Hickey
S M
SM
Trujillo
M
M
Ahmed
A
A
Olsen
K
K
McCracken
G H
GH
D000818:Animals; D003907:Dexamethasone / Q000627:therapeutic use; D006192:Haemophilus Infections / Q000628:therapy; D006801:Humans; D007223:Infant; D016753:Interleukin-10 / Q000627:therapeutic use*; D008070:Lipopolysaccharides / Q000633:toxicity; D008088:Listeriosis / Q000628:therapy; D008297:Male; D016920:Meningitis, Bacterial / Q000628:therapy*; D011817:Rabbits; D014409:Tumor Necrosis Factor-alpha / Q000096:biosynthesis
D008070:Lipopolysaccharides; D014409:Tumor Necrosis Factor-alpha; C023023:lipid-linked oligosaccharides; D016753:Interleukin-10; D003907:Dexamethasone