Evaluation of Sysmex SF-3000 performance concerning interpretive morphology flagging of the leucocyte differential count.
Results from the Sysmex SF-3000 automated haematology analyser are reported with special attention to leucocyte morphology flagging in pathological conditions. Results for leucocyte differential counts provided by the Sysmex SF-3000 were compared with those obtained from the Sysmex NE-8000 and a visual evaluation based on a differential count of 400 leucocytes. One hundred samples from apparently healthy subjects and 100 samples from patients with haematological abnormalities were examined. The SF-3000 yielded a rather high frequency of flagging inappropriately referring to blasts, immature granulocytes, left shift and nucleated red blood cells/platelet clumps. The presence of atypical lymphocytes was not indicated by appropriate flagging.
Bartels
P C
PC
Department of Clinical Chemistry, Haematology and Immunology, Medical Centre Alkmaar, Arnhem, The Netherlands.
Schoorl
M
M
Willekens
F L
FL
D003627:Data Interpretation, Statistical; D005069:Evaluation Studies as Topic; D006405:Hematology / Q000295:instrumentation*; D006801:Humans; D007958:Leukocyte Count; D012680:Sensitivity and Specificity