title: Presence of a unique parainfluenza virus 3 strain identified by RT-PCR in visna-maedi virus infected sheep.
abstract: The presence in farm sheep of a paramyxovirus closely related to parainfluenza virus type 3 (Pi3) from humans or cattle was confirmed using RT-PCR on RNA samples from lung cells from slaughtered animals. Sequencing and restriction enzyme patterns of the amplified fragment of the F gene confirms the distinctness of the isolate, and suitable PCR primers allow specific detection of the ovine virus. A study of the incidence of ovine Pi3 in samples from sheep with or without distinctive histopathological signs of maedi shows that it is uncommon in aged sheep with overt lentiviral disease, but it occurs at moderate frequency in lambs and may, in the presence of visna-maedi virus, contribute to early lesion formation.
authors.affiliation: Laboratoire d'Immunologie et de Biologie Pulmonaire, Université Claude Bernard, Lyon, France.
authors.forename: M
authors.initials: M
authors.lastname: Lyon
authors.forename: C
authors.initials: C
authors.lastname: Leroux
authors.forename: T
authors.initials: T
authors.lastname: Greenland
authors.forename: J
authors.initials: J
authors.lastname: Chastang
authors.forename: J
authors.initials: J
authors.lastname: Patet
authors.forename: J F
authors.initials: JF
authors.lastname: Mornex
mesh_terms: D000003:Abattoirs; D000818:Animals; D001483:Base Sequence; D001992:Bronchoalveolar Lavage Fluid / Q000166:cytology; D002417:Cattle; D002460:Cell Line; D018920:Coculture Techniques; D017931:DNA Primers; D004279:DNA, Viral / Q000737:chemistry / Q000235:genetics; D005814:Genes, Viral; D006801:Humans; D008168:Lung / Q000382:microbiology; D008460:Meat / Q000821:virology; D008969:Molecular Sequence Data; D010224:Parainfluenza Virus 3, Human / Q000145:classification; D016133:Polymerase Chain Reaction / Q000379:methods; D012367:RNA, Viral / Q000032:analysis; D018108:Respirovirus / Q000145:classification* / Q000254:growth & development / Q000302:isolation & purification*; D016415:Sequence Alignment; D012689:Sequence Homology, Nucleic Acid; D012756:Sheep / Q000821:virology*; D016182:Visna / Q000821:virology*; D014790:Visna-maedi virus / Q000302:isolation & purification*
chemical_list: D017931:DNA Primers; D004279:DNA, Viral; D012367:RNA, Viral