Binding of Hoechst 33258 to the TAR RNA of HIV-1. Recognition of a pyrimidine bulge-dependent structure.
The transactivation response region (TAR) RNA is an essential component in transcriptional regulation of the human immunodeficiency virus type-1 (HIV-1) genome. We have examined the interaction between TAR RNA and the bisbenzimidazole derivative Hoechst 33258. Previous studies have shown that this drug, which is well known as an AT-selective DNA minor groove binder, can also interact with GC-rich sequences in DNA as well as with RNA, possibly by intercalation. Absorption spectroscopy, circular dichroism and electric linear dichroism, as well as RNase A footprinting, were employed to compare binding of Hoechst 33258 to wild-type RNA and its analogue lacking the pyrimidine bulge. The uridine bulge, which is an important contributor to the structural stability of TAR, plays an essential role in drug binding. Deletion of the bulge destabilizes both free and drug-bound forms of TAR and markedly affects the orientation of the drug chromophore complexed with the RNA. According to the linear dichroism data, the bisbenzimidazole is oriented more or less perpendicular to the RNA helix axis. The data are compatible with a model in which the bisbenzimidazole chromophore is inserted into the existing cavity created by the pyrimidine bulge. The footprinting experiments, showing that the drug binds to a unique site opposite the unpaired uridine residues, also support this model. The binding of Hoechst 33258 to the sequence 5'-GCUCU, which delimits the cavity, reflects the greater accessibility of that region compared with other sites in the RNA molecule. The identification of a binding site for small molecules in TAR offers promising perspectives for developing drugs that would block the access of TAR RNA to proteins and therefore for the design of anti-HIV agents.
Dassonneville
L
L
Laboratoire de Pharmacologie Moléculaire Antitumorale du Centre Oscar Lambret et INSERM Unité 124, Place de Verdun, 59045 Lille, France.
Hamy
F
F
Colson
P
P
Houssier
C
C
Bailly
C
C
D000042:Absorption; D001665:Binding Sites; D006690:Bisbenzimidazole / Q000737:chemistry / Q000378:metabolism*; D002942:Circular Dichroism; D018983:DNA Footprinting; D016325:HIV Long Terminal Repeat; D015497:HIV-1 / Q000235:genetics*; D006361:Heating; D006801:Humans; D009690:Nucleic Acid Conformation; D009691:Nucleic Acid Denaturation; D009841:Oligonucleotides / Q000138:chemical synthesis / Q000378:metabolism; D011743:Pyrimidines / Q000737:chemistry; D012367:RNA, Viral / Q000737:chemistry / Q000378:metabolism*; D017384:Sequence Deletion; D013329:Structure-Activity Relationship; D014529:Uridine / Q000737:chemistry
D009841:Oligonucleotides; D011743:Pyrimidines; D012367:RNA, Viral; C030986:pyrimidine; D006690:Bisbenzimidazole; D014529:Uridine