[Ultrastructural study of the organ of Corti in animals given kanamycin and ceruloplasmin].
10 guinea pigs of group 1 were injected subcutaneously kanamycin (400 mg/kg). The other 10 guinea pigs of group 2 received human ceruloplasmin as an intraperitoneal injection of 10% solution (0.25 ml of the protein preparation per 100 g body weight) 30 min before kanamycin administration. 7 days after the treatment the cochlease were isolated and examined electron microscopically. The Corti's organ was stained with alcyan blue and lanthanum hydroxide. In animals of group 1 there was mitochondrial vacuolization, enlargement of the cisterns of the endoplasmic network, Golgi apparatus, sub-superficial network; glycocalix layer on the surface of the receptor epithelium was unbroken but uneven, in the sensor cells of the Corti's organ of the group 2 animals only few mitochondria were vacuolized. A great number of ribosomes and mitochondrial contacts with membraneous cell structures were indicative of active protein synthesis and energetic processes. Glycocalix was less damaged. This was indicative of an otoprotective effect of ceruloplasmin which diminished kanamycin ototoxicity.
Il'inskaya
E V
EV
NII ukha, nosa i rechi, Sankt-Peterburg.
Atamanova
N V
NV
D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D002570:Ceruloplasmin / Q000494:pharmacology*; D006168:Guinea Pigs; D007612:Kanamycin / Q000494:pharmacology*; D009925:Organ of Corti / Q000378:metabolism / Q000648:ultrastructure*
D000900:Anti-Bacterial Agents; D007612:Kanamycin; D002570:Ceruloplasmin