Inhibition of beta-galactosidase biosynthesis in Escherichia coli by tetracycline residues in milk.
Low levels of tetracyclines found as residues in milk inhibited the biosynthesis of beta-galactosidase in Escherichia coli. To produce the same effect, other antibacterials had to occur in concentrations that were more than 10-fold higher. This relative selectivity was exploited for the development of a screening test for tetracyclines in milk based on a chemiluminometric assay of beta-galactosidase. The method was validated with spiked samples of raw milk and applied to field samples contaminated with tetracyclines.
D'Haese
E
E
Department of Pharmaceutical Analysis, University of Ghent, Belgium.
Nelis
H J
HJ
Reybroeck
W
W
D000818:Animals; D001681:Biological Assay / Q000379:methods / Q000706:statistics & numerical data; D004926:Escherichia coli / Q000187:drug effects* / Q000201:enzymology*; D005506:Food Contamination / Q000032:analysis*; D008163:Luminescent Measurements; D008892:Milk / Q000737:chemistry*; D015203:Reproducibility of Results; D013752:Tetracycline / Q000032:analysis* / Q000494:pharmacology* / Q000633:toxicity; D001616:beta-Galactosidase / Q000096:biosynthesis*
D001616:beta-Galactosidase; D013752:Tetracycline