title: Cloning and characterization of the Flavobacterium johnsoniae (Cytophaga johnsonae) gliding motility gene, gldA.
abstract: The mechanism of bacterial gliding motility (active movement over surfaces without the aid of flagella) is not known. A large number of nonmotile mutants of the gliding bacterium Flavobacterium johnsoniae (Cytophaga johnsonae) have been previously isolated, and genetic techniques to analyze these mutants have recently been developed. We complemented a nonmotile mutant of F. johnsoniae (UW102-09) with a library of wild-type DNA by using the shuttle cosmid pCP17. The complementing plasmid (pCP100) contained an insert of 13 kbp, and restored motility to 4 of 61 independently isolated nonmotile mutants. A 1.3-kbp fragment that encompassed a single ORF, gldA, complemented all four mutants. Disruption of the chromosomal copy of gldA in wild-type F. johnsoniae UW101 eliminated gliding motility. The predicted protein produced by gldA has strong sequence similarity to ATP binding cassette transport proteins.
authors.affiliation: Department of Biological Sciences, University of Wisconsin, P.O. Box 413, Milwaukee, WI 53201, USA.
authors.forename: S
authors.initials: S
authors.lastname: Agarwal
authors.forename: D W
authors.initials: DW
authors.lastname: Hunnicutt
authors.forename: M J
authors.initials: MJ
authors.lastname: McBride
mesh_terms: D018528:ATP-Binding Cassette Transporters / Q000235:genetics*; D000595:Amino Acid Sequence; D001419:Bacteria / Q000235:genetics* / Q000821:virology; D001426:Bacterial Proteins / Q000235:genetics; D005417:Flavobacterium / Q000235:genetics* / Q000821:virology; D015723:Gene Library; D005798:Genes, Bacterial; D005816:Genetic Complementation Test; D008969:Molecular Sequence Data; D009068:Movement / Q000502:physiology*; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid
chemical_list: D018528:ATP-Binding Cassette Transporters; D001426:Bacterial Proteins; C109038:GldA protein, bacteria