Chromosomal aberrations in vitro induced by aneugens.
Various aneugens were reported to induce structural chromosomal aberrations beside their influence on cell division and their aneugenic potential To asses, whether a relationship between disturbance of cell division and clastogenic potential exists, CHO cells were treated with the well-known aneugens colcemid, colchicine and vincristine and investigated for the induction of structural chromosomal aberrations, polyploid cells and alterations in mitotic index. At low and intermediate concentration, all compounds induced polyploidy and an increase in mitotic index, but no structural aberrations at all. However, at high concentrations, colcemid and colchicine both induced numerous structural chromosomal aberrations in diploid cells. Colchicine was also clastogenic in tetraploid cells. Vincristine did not induce structural chromosomal aberrations in diploid cells, but in tetraploid cells. The clastogenic effects showed a clear-cut threshold with all three compounds. Furthermore, it was found that the tetraploid condition in CHO cells is generally accompanied by an increase in structural chromosomal aberrations, in vehicle controls as well as in cultures treated with the aneugens. Nevertheless, this study demonstrates that for the three aneugenic compounds tested, no direct relationship between compound induced disturbance of cell cycle and compound induced structural chromosomal aberration incidence exists.
Arni
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P
Novartis Crop Protection AG, Basel, Switzerland.
Hertner
T
T
D000782:Aneuploidy; D000818:Animals; D016466:CHO Cells; D003078:Colchicine / Q000633:toxicity; D006224:Cricetinae; D003703:Demecolcine / Q000633:toxicity; D004305:Dose-Response Relationship, Drug; D009152:Mutagenicity Tests; D009153:Mutagens; D013997:Time Factors; D014750:Vincristine / Q000633:toxicity
D009153:Mutagens; D014750:Vincristine; D003078:Colchicine; D003703:Demecolcine