Phenotype and genotype of Candida albicans strains isolated from pregnant women with recurrent vaginitis.
Fourteen out of 80 pregnant women receiving prenatal care presented signs and symptoms of recurrent vaginal candidiasis. Candida albicans strains were isolated from 12 patients (85.7%), and these were submitted to morphotyping (morphological characteristics of the colony), antifungal typing (pattern of sensitivity to amphotericin B, 5-fluorcytosine, myconazole, ketoconazole and fluconazole) and genotyping (electrophoretic migration of DNA fragments digested with EcoRI and HinfI). Alteration of morphotype and antifungal type was observed in 50% of the patients, but the genotype of the strains isolated from the same patients at different times was identical in all subjects. The predominant morphotypes presented continuous fringes and the basic changes observed among antifungal types was the emergence of strains resistant to myconazole, which was the drug used for the treatment of the first episode of vaginitis. We conclude that recurrent vaginal candidasis is caused by the persistence of a single yeast genotype that undergoes morphological and behavioral changes in the presence of antifungal agents due to the selective pressure to which it is submitted.
Maffei
C M
CM
Departamento de Parasitologia, Microbiologia e Imunologia da Faculdade de Medicina de Ribeirão Preto da Universidade de São Paulo. cmlmaffe@fmrp.usp.br
Paula
C R
CR
Mazzocato
T S
TS
Franceschini
S
S
D002176:Candida albicans / Q000145:classification* / Q000166:cytology / Q000235:genetics; D002181:Candidiasis, Vulvovaginal / Q000382:microbiology*; D016172:DNA Fingerprinting; D005260:Female; D005838:Genotype; D006801:Humans; D016533:Mycological Typing Techniques; D010641:Phenotype; D011247:Pregnancy; D011251:Pregnancy Complications, Infectious / Q000382:microbiology*; D012008:Recurrence; D014627:Vaginitis / Q000382:microbiology*