Purification, characterization, and amino acid sequencing of DNase gamma from rat spleen.
An endonuclease named DNase gamma was purified to apparent homogeneity from rat splenocyte nuclei and its properties were characterized. We also determined the NH2-terminal and partial amino acid sequences of the proteolytic internal peptides. The molecular mass of gamma DNase was 33,000 daltons as determined by SDS-polyacrylamide gel electrophoresis. A native molecular mass of 30,000 was estimated by gel filtration. Purified DNase gamma is active in the presence of both Ca2+ and Mg2+ or Mn2+ alone and inhibited by Co2+, Ni2+, Cu2+, and especially Zn2+. Maximal activity was achieved at pH 7.2 in Mops-NaOH buffer. The sequence data on the NH2-terminal and seven internal peptides obtained by sequential digestions with Achromobacter protease I and endoproteinase Asp-N revealed that DNase gamma is a novel endonuclease that shows sequence homology with DNase I.
Shiokawa
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D
Department of Biochemistry, Faculty of Pharmaceutical Sciences, Science University of Tokyo, Japan.
Iwamatsu
A
A
Tanuma
S
S
D000595:Amino Acid Sequence; D000818:Animals; D002118:Calcium / Q000494:pharmacology; D002413:Cations, Divalent / Q000494:pharmacology; D002851:Chromatography, High Pressure Liquid; D003850:Deoxyribonuclease I / Q000737:chemistry / Q000145:classification; D004706:Endodeoxyribonucleases / Q000737:chemistry* / Q000145:classification / Q000187:drug effects / Q000378:metabolism; D008274:Magnesium / Q000494:pharmacology; D008297:Male; D008969:Molecular Sequence Data; D051381:Rats; D017208:Rats, Wistar; D017421:Sequence Analysis; D017386:Sequence Homology, Amino Acid; D013154:Spleen / Q000201:enzymology*
D002413:Cations, Divalent; D004706:Endodeoxyribonucleases; C105124:deoxyribonuclease gamma; D003850:Deoxyribonuclease I; D008274:Magnesium; D002118:Calcium