Relationship between size of injected microspheres and ultrasound imaging of heart by PLSR.
OBJECTIVE
To investigate the enhancement in ultrasound signal (US) in heart, for diagnostic purposes, as a function of size and number of injected air-filled microspheres by multivariate statistical methods.


METHODS
The US enhancement in left ventricle was measured after injection of 31 suspensions of air-filled albumin microspheres divided on and injected in six dogs. The relationship between the size of microspheres between 1-38 microns and the US enhancement was explored by Pearson's product moment correlation analysis, ordinary least-squares regression, principal component regression (PCR) and partial least-squares regression (PLS).


RESULTS
Relative advanced algorithms such as PCR and PLS were required to achieve accurate in vivo/in vitro correlation. The most effective microsphere sizes contributing to US enhancement in left ventricle in dogs were estimated to be in the 7-15 microns range.


CONCLUSIONS
In general, the effective in vivo sizes are dependent on the type of formulation due to the surprisingly large active in vivo sizes found for the tested concept. PCR and PLS are suitable methods for in vivo/in vitro correlation, especially for covariated and noisy data.
Dyrstad
K
K
Pharmaceutical Sciences, Research and Development Section, Nycomed Imaging AS, Oslo, Norway. Knut.dyrstad@nycomed.telemax.no
Sontum
P C
PC
D000418:Albumins; D000465:Algorithms; D000818:Animals; D004285:Dogs; D004452:Echocardiography / Q000379:methods*; D006801:Humans; D007089:Image Enhancement; D008863:Microspheres; D015999:Multivariate Analysis; D010316:Particle Size; D012044:Regression Analysis
D000418:Albumins