Microchannel electrophoretic separations of DNA in injection-molded plastic substrates.
Microfabricated electrophoretic separation devices have been produced by an injection-molding process. The strategy for producing the devices involved solution-phase etching of a master template on a silicon wafer, followed by electroforming more durable injection-molding masters in nickel from the silicon master. One of the nickel electroforms was then used to prepare an injection mold insert, from which microchannel chips in an acrylic substrate were mass-produced. The microchannel devices were used to demonstrate high-resolution separations of double-stranded DNA fragments with total run times of less than 3 min. Run-to-run and chip-to-chip reproducibility was good, with relative standard deviation values below 1% for the run-to-run data and in the range of 2-3% for the chip-to-chip comparisons. Such devices could lead to the production of low-cost, single-use electrophoretic chips suitable for a variety of separation applications, including DNA sizing, DNA sequencing, random primary library screening, and rapid immunoassay testing.
McCormick
R M
RM
Soane BioSciences, Hayward, California 94545-3716, USA.
Nelson
R J
RJ
Alonso-Amigo
M G
MG
Benvegnu
D J
DJ
Hooper
H H
HH
D010584:Bacteriophage phi X 174 / Q000235:genetics; D004247:DNA / Q000302:isolation & purification*; D004279:DNA, Viral / Q000302:isolation & purification; D004586:Electrophoresis / Q000295:instrumentation* / Q000379:methods; D010969:Plastics; D011108:Polymers; D015203:Reproducibility of Results
D004279:DNA, Viral; D010969:Plastics; D011108:Polymers; D004247:DNA