Cross-linked trimers of bovine ribonuclease A: activity on double-stranded RNA and antitumor action.
Trimers of bovine pancreatic RNase A were obtained by cross-linking native RNase A with dimethyl suberimidate. They degrade double-stranded RNA more efficiently than dimers and monomers of RNase A, and display significant cytotoxic and/or cytostatic actions against C4-I cells (a human cell line derived from squamous carcinoma of the uterus cervix). On the same cell line cross-linked dimers of RNase A appear to be ineffective.
Gotte
G
G
Institute of Biological Chemistry, Faculty of Medicine, The University of Verona, Italy.
Testolin
L
L
Costanzo
C
C
Sorrentino
S
S
Armato
U
U
Libonati
M
M
D000818:Animals; D000970:Antineoplastic Agents / Q000737:chemistry* / Q000378:metabolism / Q000494:pharmacology*; D002417:Cattle; D002470:Cell Survival / Q000187:drug effects; D002850:Chromatography, Gel; D003432:Cross-Linking Reagents; D019281:Dimerization; D004120:Dimethyl Suberimidate; D006801:Humans; D009690:Nucleic Acid Conformation; D010179:Pancreas / Q000201:enzymology; D011487:Protein Conformation; D012330:RNA, Double-Stranded / Q000378:metabolism*; D012259:Ribonuclease, Pancreatic / Q000737:chemistry* / Q000378:metabolism* / Q000494:pharmacology; D014407:Tumor Cells, Cultured
D000970:Antineoplastic Agents; D003432:Cross-Linking Reagents; D012330:RNA, Double-Stranded; D004120:Dimethyl Suberimidate; D012259:Ribonuclease, Pancreatic