Glutathione pretreatment lessens the acute liver injury induced by carbon tetrachloride.
We explored the protective activity of glutathione (GSH) in a rat model of carbon tetrachloride-induced acute liver damage. Histological examination of livers from GSH-pretreated rats revealed minor damage, confirmed by biochemical parameters of liver cell necrosis evaluated both 24 and 48 hr after hepatotoxin delivery. In addition we quantified changes in hepatic steady-state levels of albumin, type I procollagen, transforming growth factor-beta 1 and tumour necrosis factor-alpha mRNAs. Even at the molecular level and above all for the albumin gene, it appears that GSH lessens the effect of the hepatotoxin, however the protection of the thiol is restricted to the first 24 hrs and is almost totally exhausted after 48 hr. Since, only 24 hr after CC14 delivery, GSH abundance determined in erythrocytes and liver is almost equal in the controls and in the GSH injected rats, but significantly higher than in the only intoxicated animals (P < 0.05, intraerythrocyte content), we conclude that the thiol pretreatment exerts an effective but transient protection.
Arosio
B
B
Institute of Internal Medicine, University of Milano, Ospedale Maggiore IRCCS, Italy.
Santambrogio
D
D
Gagliano
N
N
Ryan
A
A
Biasi
F
F
Vergani
C
C
Annoni
G
G
D000818:Animals; D015415:Biomarkers / Q000097:blood; D015152:Blotting, Northern; D002251:Carbon Tetrachloride / Q000633:toxicity*; D004912:Erythrocytes / Q000378:metabolism; D005978:Glutathione / Q000008:administration & dosage* / Q000032:analysis; D008099:Liver / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D008297:Male; D009336:Necrosis; D011347:Procollagen / Q000097:blood / Q000235:genetics; D012333:RNA, Messenger / Q000032:analysis; D051381:Rats; D017207:Rats, Sprague-Dawley; D012709:Serum Albumin / Q000235:genetics / Q000378:metabolism; D016212:Transforming Growth Factor beta / Q000097:blood / Q000235:genetics; D014409:Tumor Necrosis Factor-alpha / Q000235:genetics / Q000378:metabolism
D015415:Biomarkers; D011347:Procollagen; D012333:RNA, Messenger; D012709:Serum Albumin; D016212:Transforming Growth Factor beta; D014409:Tumor Necrosis Factor-alpha; D002251:Carbon Tetrachloride; D005978:Glutathione