Chronic renal blood flow measurement in dogs by transit-time ultrasound flowmetry.
To test the validity of transit-time ultrasound flowmetry for chronic measurement of renal blood flow in dogs, we compared this method with the renal clearance of para-aminohippuric acid (CPAH) (corrected for hematocrit), and with direct volumetric measurements. When flow-probes were implanted without silastic sheeting to stabilize the implant, there was significant disparity between the (within-dog) mean levels of renal blood flow estimated by flow-probe and CPAH. In contrast, when the flow-probe implants were stabilized with silicone sheeting, there was close agreement in each dog between the flow rates measured by the two methods. When flow-probes were calibrated volumetrically in situ, there was a close linear relationship between flow derived from the flow-probe and that measured volumetrically (r = 0.98 +/- 0.02). We conclude that valid, chronic measurement of renal blood flow in dogs can be achieved using transit-time ultrasound flowmetry, provided the implant is stabilized with silicone sheeting.
Evans
R G
RG
Baker Medical Research Institute, Prahran, Victoria, Australia. roger.evans@med.monash.edu.au
Stevenson
K M
KM
Malpas
S C
SC
Fitzgerald
S M
SM
Shweta
A
A
Tomoda
F
F
Anderson
W P
WP
D000818:Animals; D001783:Blood Flow Velocity; D004285:Dogs; D007668:Kidney / Q000098:blood supply*; D007677:Kidney Function Tests / Q000379:methods; D008297:Male; D019736:Prostheses and Implants; D012079:Renal Circulation / Q000502:physiology*; D015203:Reproducibility of Results; D012212:Rheology / Q000379:methods; D012826:Silicone Elastomers; D014463:Ultrasonography / Q000379:methods*; D010130:p-Aminohippuric Acid / Q000493:pharmacokinetics
D012826:Silicone Elastomers; D010130:p-Aminohippuric Acid