[A study for serodiagnosis of verotoxin-producing Escherichia coli (enterohemorrhagic E. coli) O157 by the bacterial agglutination technique].
We evaluated the usefulness of bacterial agglutination antibodies for serodiagnosis of verotoxin-producing Escherichia coli (enterohemorrhagic E. coli) O157 infections. We examined 50 serum samples from 50 control children (whiout diarrhea 31, with diarrhea 19), 24 samples from 8 diarrhea cases due to O157:H7, 37 samples from 14 cases of hemolytic uremic syndrome (HUS) for antibodies to heat-killed E. coli E32511 (O157:H.-) strain using the bacterial agglutination technique. Of the control sera all but one (x80) showed 20 > or = in the antibody. All the diarrhea patients due to O157:H7 showed a significant rise (x160-x5120) of the titers in the sera at 5-7 days on illness, after that the titers fell rapidly. Significant antibody rise (x160-x5120) was detected in twelve out of 14 HUS patients at the early stage of the illness which fell in the convalescent phase. The assay appeared to be a useful serodiagnostic technique because of its easiness and simplicity as well as because of its high sensitivity and specificity.
Morooka
T
T
Department of Pediatrics, Chikushi Hospital, Fukuoka University.
Yamaguchi
T
T
Horikawa
K
K
Matano
H
H
Hirota
O
O
Inoue
S
S
Tanaka
M
M
D000372:Agglutination Tests / Q000379:methods*; D001427:Bacterial Toxins / Q000096:biosynthesis; D002648:Child; D002675:Child, Preschool; D004768:Enterotoxins / Q000096:biosynthesis; D004927:Escherichia coli Infections / Q000175:diagnosis*; D019453:Escherichia coli O157 / Q000145:classification*; D006801:Humans; D007223:Infant; D012698:Serologic Tests / Q000379:methods; D022622:Shiga Toxin 1
D001427:Bacterial Toxins; D004768:Enterotoxins; D022622:Shiga Toxin 1