Lung neutrophil retention and injury after intestinal ischemia/reperfusion.
OBJECTIVE
To define the mechanisms responsible for the lung leukosequestration and injury elicited by intestinal ischemia/reperfusion (I/R).


METHODS
The effect of 120 minutes of superior mesenteric artery occlusion and 90 minutes of reperfusion on neutrophil deformability, lung neutrophil retention, and pulmonary microvascular permeability was determined.


RESULTS
Compared with control surgery, intestinal I/R resulted in a significant increase in neutrophil stiffness (mean yield pressure [Pyield], 1.533 +/- 0.075 and 2.302 +/- 0.288 cm H2O, respectively) and lung neutrophil content (6.3 +/- 1.4 and 31.5 +/- 6.4 U/g wet weight, respectively). These changes were not affected by inhibition of neutrophil adherence before gut reperfusion. However, the increased lung microvascular permeability elicited by gut I/R (0.111 +/- 0.020 [control surgery] and 0.255 +/- 0.041 [I/R] mL/min/cm H2O/100 g lung tissue) was significantly attenuated by administration of antibodies directed against neutrophil or endothelial determinants of leukocyte adhesion.


CONCLUSIONS
The results of this study suggest that intestinal I/R is a potent inflammatory stimulus that elicits an increase in neutrophil stiffness and lung neutrophil retention independent of neutrophil-endothelial cell adhesion. In contrast, the increased lung microvascular permeability elicited by gut I/R is attenuated by strategies that interfere with neutrophil-endothelial cell adhesion.
Xiao
F
F
Department of Medicine, Louisiana State University Medical Center, Shreveport 71130, USA.
Eppihimer
M J
MJ
Young
J A
JA
Nguyen
K
K
Carden
D L
DL
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D015703:Antigens, CD / Q000276:immunology; D002199:Capillary Permeability / Q000502:physiology; D002448:Cell Adhesion / Q000502:physiology; D048429:Cell Size / Q000502:physiology; D018799:Intercellular Adhesion Molecule-1 / Q000276:immunology; D007413:Intestinal Mucosa / Q000378:metabolism*; D019041:L-Selectin / Q000276:immunology; D008168:Lung / Q000201:enzymology / Q000473:pathology*; D008833:Microcirculation / Q000502:physiology; D009504:Neutrophils / Q000502:physiology*; D009844:Oligosaccharides / Q000276:immunology; D009195:Peroxidase / Q000378:metabolism; D051381:Rats; D015427:Reperfusion Injury / Q000473:pathology* / Q000503:physiopathology; D000080506:Sialyl Lewis X Antigen
D000911:Antibodies, Monoclonal; D015703:Antigens, CD; D009844:Oligosaccharides; D000080506:Sialyl Lewis X Antigen; D018799:Intercellular Adhesion Molecule-1; D019041:L-Selectin; D009195:Peroxidase