Plasma-modified nylon meshes as supports for cell culturing.
The polymeric surfaces of three commercially available nylon films with mesh openings of 5 microns, 10 microns and 20 microns were treated with anhydrous ammonia gaseous plasma. Cells cultured on the plasma-treated nylon films have higher proliferation rate and assume morphology distinct from those cultured on the unmodified films. Of the three plasma-modified membranes, the one with 5 microns mesh openings supported a largest population of cell growth. The plasma-treated nylon meshes provided a stronger anchorage for the collagen matrices formed within the mesh openings. Application of this collagen/nylon meshes for cell culturing is demonstrated.
Kuo
S M
SM
Institute of Biomedical Engineering, National Yang Ming University, Shi Pai, Pei Tou, Taipei, Taiwan, ROC.
Tsai
S W
SW
Huang
L H
LH
Wang
Y J
YJ
D016475:3T3 Cells; D000641:Ammonia; D000812:Anhydrides; D000818:Animals; D018929:Cell Culture Techniques / Q000379:methods*; D003094:Collagen; D008567:Membranes, Artificial; D051379:Mice; D008855:Microscopy, Electron, Scanning; D009757:Nylons / Q000378:metabolism; D010949:Plasma
D000812:Anhydrides; D008567:Membranes, Artificial; D009757:Nylons; D000641:Ammonia; D003094:Collagen