In vitro effect of garlic powder extract on lipid content in normal and atherosclerotic human aortic cells.
In the present study, the mechanism of the in vitro effect of garlic powder extract (GPE) on lipid content of cultured human aortic cells was investigated. The addition of GPE abolished atherogenic blood serum-induced accumulation of free cholesterol, triglycerides, and cholesteryl esters in smooth muscle cells derived from uninvolved (normal) intima. In cells isolated from atherosclerotic plaque, GPE lowered these lipids. GPE inhibited lipid synthesis both in normal and atherosclerotic cells. It inhibited acyl-CoA:cholesterol acyltransferase activity that participates in the cholesteryl ester formation and stimulated cholesteryl ester hydrolase that degrades cholesteryl esters. This may explain the lipid reduction caused by GPE in atherosclerotic cells. GPE inhibited the uptake of modified low density lipoprotein and degradation of lipoprotein-derived cholesteryl esters, thus considerably reducing the intracellular accumulation of cholesteryl esters. This suggests the mechanism responsible for the prevention of lipid accumulation in aortic cells caused by atherogenic blood serum.
Orekhov
A N
AN
Institute of Experimental Cardiology, Russian Academy of Medical Sciences, Moscow, Russia.
Tertov
V V
VV
D000328:Adult; D001011:Aorta / Q000187:drug effects* / Q000201:enzymology / Q000473:pathology; D001161:Arteriosclerosis / Q000378:metabolism*; D004730:Endothelium, Vascular / Q000187:drug effects* / Q000201:enzymology / Q000473:pathology; D005737:Garlic / Q000737:chemistry*; D006801:Humans; D050356:Lipid Metabolism; D008074:Lipoproteins / Q000378:metabolism; D008297:Male; D008875:Middle Aged; D010936:Plant Extracts / Q000494:pharmacology*; D010946:Plants, Medicinal; D011208:Powders; D002787:Sterol Esterase / Q000378:metabolism; D002785:Sterol O-Acyltransferase / Q000037:antagonists & inhibitors / Q000378:metabolism
D008074:Lipoproteins; D010936:Plant Extracts; D011208:Powders; D002785:Sterol O-Acyltransferase; D002787:Sterol Esterase