title: Subcellular localization of dihydropyrimidine dehydrogenase.
abstract: Although dihydropyrimidine dehydrogenase (DPD) has been purified and characterized from liver tissues of various mammals conflicting data exist on its subcellular localization. To determine the localization of DPD we prepared crude subcellular fractions of a rat liver homogenate by means of differential centrifugation. In the fractions obtained (heavy mitochondrial, light mitochondrial, microsomal and cytosolic) the activities of different marker enzymes were measured as well as the activity of DPD. These results showed that almost all of the activity of DPD was located in the cytosolic fraction. To exclude any particulate-associated DPD, a light mitochondrial fraction was subsequently subjected to equilibrium density gradient centrifugation. The distribution profile of the activity of DPD and the various marker enzymes indicated that DPD from rat liver was exclusively located in the cytosol since no significant activity of DPD could be detected in any subcellular organelle.
authors.affiliation: Academic Medical Center, University of Amsterdam, Emma Children's Hospital, The Netherlands.
authors.forename: A B
authors.initials: AB
authors.lastname: Van Kuilenburg
authors.forename: H
authors.initials: H
authors.lastname: Van Lenthe
authors.forename: R J
authors.initials: RJ
authors.lastname: Wanders
authors.forename: A H
authors.initials: AH
authors.lastname: Van Gennip
mesh_terms: D000818:Animals; D002374:Catalase / Q000032:analysis / Q000378:metabolism; D002499:Centrifugation, Density Gradient; D003600:Cytosol / Q000201:enzymology; D042943:Dihydrouracil Dehydrogenase (NADP); D005969:Glutamate Dehydrogenase / Q000032:analysis / Q000378:metabolism; D007770:L-Lactate Dehydrogenase / Q000032:analysis / Q000378:metabolism; D008099:Liver / Q000201:enzymology* / Q000648:ultrastructure; D008297:Male; D008862:Microsomes, Liver / Q000201:enzymology; D008930:Mitochondria, Liver / Q000201:enzymology; D010088:Oxidoreductases / Q000032:analysis / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar
chemical_list: D010088:Oxidoreductases; D007770:L-Lactate Dehydrogenase; D002374:Catalase; D042943:Dihydrouracil Dehydrogenase (NADP); D005969:Glutamate Dehydrogenase