Prostate specific antigen reverse transcriptase-polymerase chain reaction assay in preoperative staging of prostate cancer.
OBJECTIVE
Extracapsular extension of prostate cancer occurs in a significant number of men believed to have clinically localized disease. We report the ability of the reverse transcriptase-polymerase chain reaction (RT-PCR) assay to predict preoperatively the pathological stage of cases of clinically localized prostate cancer.


METHODS
Since October 1994, 82 consecutive men with clinically localized prostate cancer had a venous blood RT-PCR assessment before radical retropubic prostatectomy. The extracted ribonucleic acid was reverse transcribed, amplified and the amplicon identity confirmed by prostate specific antigen (PSA) directed probe hybridization. An additional 31 patients were enrolled to provide appropriate positive (T + Nx/1M2) and negative (human female and benign prostatic hyperplasia) controls. Histological examination of the entire prostatectomy specimen was performed.


RESULTS
Positive RT-PCR assay results correlated significantly with skeletal metastases and elevated levels of serum PSA but they did not significantly improve our ability to identify prospectively patients with extracapsular extension over traditional predictors (serum PSA, Gleason score).


CONCLUSIONS
The role of molecular techniques in prostate cancer evaluation and prognosis continues to emerge. However, in our study we demonstrate no significant advantage in preoperative staging of prostate cancer using RT-PCR assay with PSA primers.
Ignatoff
J M
JM
Department of Urology, Evanston Hospital, Illinois, USA.
Oefelein
M G
MG
Watkin
W
W
Chmiel
J S
JS
Kaul
K L
KL
D000368:Aged; D006801:Humans; D016015:Logistic Models; D008297:Male; D008875:Middle Aged; D009367:Neoplasm Staging; D016133:Polymerase Chain Reaction; D011237:Predictive Value of Tests; D011300:Preoperative Care; D011446:Prospective Studies; D017430:Prostate-Specific Antigen / Q000032:analysis* / Q000235:genetics; D011471:Prostatic Neoplasms / Q000097:blood / Q000473:pathology*; D012313:RNA / Q000032:analysis; D012680:Sensitivity and Specificity
D012313:RNA; D017430:Prostate-Specific Antigen