title: Pregnancy following intracytoplasmic sperm injection of immotile spermatozoa selected by the hypo-osmotic swelling-test: a case report.
abstract: The success of intracytoplasmic sperm injection (ICSI) is poor when only immotile spermatozoa can be retrieved. In a couple with complete male asthenozoospermia the possible use of the hypo-osmotic swelling test to select spermatozoa for microinjection was examined. Following incubation in hypo-osmotic medium (Hypo 10, IVF Science, Göteborg, Sweden), 26% of immotile spermatozoa showed signs of sperm swelling (HOS-positive). After injection of HOS-positive spermatozoa, 5 out of 12 oocytes fertilized (41%) and after transfer of three embryos a healthy singleton pregnancy was achieved. In a previous ICSI cycle of this couple without preselection of spermatozoa by the HOS test, only 1 out of 10 oocytes fertilized. It is concluded that selection of spermatozoa by hypo-osmotic swelling-test prior to sperm microinjection seems to be a valuable tool to increase the fertilization rate in cases with complete asthenozoospermia.
authors.affiliation: Department of Endocrinology and Reproductive Medicine, University of Bonn, Germany.
authors.forename: S
authors.initials: S
authors.lastname: Ved
authors.forename: M
authors.initials: M
authors.lastname: Montag
authors.forename: A
authors.initials: A
authors.lastname: Schmutzler
authors.forename: G
authors.initials: G
authors.lastname: Prietl
authors.forename: G
authors.initials: G
authors.lastname: Haidl
authors.forename: H
authors.initials: H
authors.lastname: van der Ven
mesh_terms: D000328:Adult; D002469:Cell Separation / Q000379:methods*; D048429:Cell Size; D004624:Embryo Transfer; D005260:Female; D005307:Fertilization in Vitro / Q000379:methods*; D006801:Humans; D007038:Hypotonic Solutions; D007248:Infertility, Male / Q000209:etiology / Q000628:therapy*; D008297:Male; D008845:Microinjections; D011247:Pregnancy; D013081:Sperm Motility
chemical_list: D007038:Hypotonic Solutions