Application of multiplex polymerase chain reaction for rapid identification of Campylobacter jejuni and C coli associated with reproductive failure.
OBJECTIVE
To evaluate a multiplex polymerase chain reaction (PCR) to distinguish Campylobacter jejuni from C coli as causes of reproductive failure.


METHODS
Review of clinical cases of reproductive failure attributed to C jejuni or C coli.


RESULTS
A case of swine abortion was attributable to infection with C coli. The porcine abortion isolates were verified as C coli by restriction fragment length polymorphism and multiplex PCR. Cases of endometritis in a fox and in mink caused by C jejuni were reviewed, and isolates were confirmed as C jejuni by results of the multiplex PCR.


CONCLUSIONS
Multiplex PCR was useful in identifying C coli and C jejuni recovered from atypical cases of reproductive failure. Multiplex PCR in conjunction with conventional assays may be useful for verifying other unusual instances of campylobacteriosis.
Wesley
I V
IV
Enteric Diseases and Food Safety Research Unit, National Animal Disease Center, USDA, Ames, IA 50010, USA.
Sanderson
T P
TP
Larson
D J
DJ
Harmon
K M
KM
Andrews
J J
JJ
Miskimins
D W
DW
Zeman
D H
DH
D000031:Abortion, Septic / Q000382:microbiology / Q000503:physiopathology / Q000662:veterinary; D000034:Abortion, Veterinary / Q000382:microbiology / Q000503:physiopathology; D000818:Animals; D001483:Base Sequence; D015139:Blotting, Southern / Q000379:methods / Q000662:veterinary; D002169:Campylobacter Infections / Q000150:complications / Q000175:diagnosis / Q000662:veterinary*; D017000:Campylobacter coli / Q000235:genetics / Q000302:isolation & purification* / Q000502:physiology; D016123:Campylobacter jejuni / Q000235:genetics / Q000302:isolation & purification* / Q000502:physiology; D004269:DNA, Bacterial / Q000032:analysis / Q000737:chemistry / Q000235:genetics; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods / Q000662:veterinary; D004716:Endometritis / Q000382:microbiology / Q000503:physiopathology / Q000662:veterinary; D005260:Female; D005589:Foxes; D008907:Mink; D009841:Oligonucleotides / Q000032:analysis / Q000737:chemistry / Q000235:genetics; D016133:Polymerase Chain Reaction / Q000379:methods / Q000662:veterinary*; D012150:Polymorphism, Restriction Fragment Length; D011247:Pregnancy; D012098:Reproduction / Q000502:physiology; D013552:Swine; D013553:Swine Diseases / Q000175:diagnosis* / Q000382:microbiology / Q000503:physiopathology
D004269:DNA, Bacterial; D009841:Oligonucleotides