title: Organisation of ribosomal DNA in the ascomycete Leptosphaeria maculans.
abstract: In the ascomycete Leptosphaeria maculans tandem repeats of ribosomal DNA (rDNA) are restricted to one or two particular chromosomes of the 15 chromosomes of 19 field isolates examined. Ribosomal DNA can account for size differences of 35% between homologous chromosomes in a particular tetrad. During crossing, no detectable recombination between blocks of tandem repeats, nor changes in their size occur. The organisation of rDNA in L. maculans differs from many other haploid fungi. Firstly, sequence heterogeneity occurs within tandem repeats of rDNA; regularly spaced Sal 1 sites (0.25 Mb apart) are present within a 1.4 Mb block of tandem repeats. Secondly, individual isolates have different-sized rDNA repeats; this variation occurs in the non-transcribed intergenic spacer region. Thirdly, there is a wide range in the copy number of the rDNA repeat (from 56 to 225) amongst only four field isolates examined.
authors.affiliation: Plant Cell Biology Research Centre, School of Botany, University of Melbourne, Parkville, Vic., Australia.
authors.forename: B J
authors.initials: BJ
authors.lastname: Howlett
authors.forename: B D
authors.initials: BD
authors.lastname: Rolls
authors.forename: A J
authors.initials: AJ
authors.lastname: Cozijnsen
mesh_terms: D001203:Ascomycota / Q000235:genetics*; D015825:Chromosomes, Fungal; D004271:DNA, Fungal / Q000737:chemistry*; D004275:DNA, Ribosomal / Q000737:chemistry*; D012091:Repetitive Sequences, Nucleic Acid
chemical_list: D004271:DNA, Fungal; D004275:DNA, Ribosomal