Mutations of the p53 gene in human lung cancer from chromate-exposed workers.
We examined p53 mutations in 20 cancer samples from 19 chromate workers with lung cancer by Polymerase chain reaction-Single strand conformation polymorphism analysis and direct sequencing. Six missense mutations were identified in 4 (20%) of the 20 chromate lung cancer samples. Fewer mutations were found in the patients with lung cancers who had been exposed to chromate than in those who had not. However, the pattern of p53 mutations in lung cancer patients exposed to chromate differed from that of common lung cancers in 3 respects. There were no apparent G to T transversions, which are common base changes in lung cancers. Half of the mutational sites (3/ 6) had changes of AT base-pairs, and 2 of 4 mutational tumor samples had double missense mutations. Our results suggested that chromate exposure may induce point mutation of the p53 gene.
Kondo
K
K
Second Department of Surgery, School of Medicine, University of Tokushima, Japan. kondo@clin.med.tokushima-u.ac.jp
Hino
N
N
Sasa
M
M
Kamamura
Y
Y
Sakiyama
S
S
Tsuyuguchi
M
M
Hashimoto
M
M
Uyama
T
T
Monden
Y
Y
D000230:Adenocarcinoma / Q000139:chemically induced; D000368:Aged; D018288:Carcinoma, Small Cell / Q000139:chemically induced; D002294:Carcinoma, Squamous Cell / Q000139:chemically induced; D002840:Chromates / Q000009:adverse effects*; D006801:Humans; D008175:Lung Neoplasms / Q000139:chemically induced* / Q000235:genetics*; D008875:Middle Aged; D016273:Occupational Exposure; D017354:Point Mutation; D016133:Polymerase Chain Reaction; D018807:Polymorphism, Single-Stranded Conformational; D017422:Sequence Analysis, DNA; D016159:Tumor Suppressor Protein p53 / Q000235:genetics*
D002840:Chromates; D016159:Tumor Suppressor Protein p53