Dual temperature model for the estimation of energetics parameters for acetylcholinesterase inhibition by cyclophosphamide.
The present work addresses the 'dual temperature' model for the estimation of Gibb's free energy change (delta G), enthalpy change (delta H), heat of activation (delta H.) entropy change (delta S), temperature coefficient (Q 10) and activation energy (Ea) of chicken brain acetylcholinesterase (AChE) inhibited by cyclophosphamide monohydrate (CP). In this investigation, the PZ factor (number of sterically and energy wise favorable collisions occurring between CP and AChE) has also been studied. The inhibitor has considerably increased all energetics parameters except delta S and Q10. These results are in general agreement with the data from the other reported studies. The significance of the use of CP in cancer therapy and various aspects of thermodynamic parameters have also been discussed.
Kamal
M A
MA
Department of Biochemistry, College of Science, King Saud University, Riyadh, Saudi Arabia.
D000110:Acetylcholinesterase / Q000187:drug effects* / Q000378:metabolism; D000477:Alkylating Agents / Q000494:pharmacology*; D000818:Animals; D001921:Brain / Q000201:enzymology; D002645:Chickens; D002800:Cholinesterase Inhibitors / Q000494:pharmacology*; D003520:Cyclophosphamide / Q000494:pharmacology*; D005260:Female; D008297:Male; D008954:Models, Biological; D008956:Models, Chemical; D013696:Temperature
D000477:Alkylating Agents; D002800:Cholinesterase Inhibitors; D003520:Cyclophosphamide; D000110:Acetylcholinesterase