title: Effects of dobutamine stress on myocardial blood flow, 99mTc sestamibi uptake, and systolic wall thickening in the presence of coronary artery stenoses: implications for dobutamine stress testing.
abstract: BACKGROUND
  Although dobutamine stress is used with both 99mTc sestamibi (sestamibi) myocardial perfusion imaging and echocardiography for detecting coronary artery stenoses, the impact of stenosis severity on test end points (myocardial sestamibi uptake and systolic thickening, respectively) has not been clearly defined.
  
  
  RESULTS
  In 15 open-chest dogs, dobutamine (2.5 to 30 microg x kg(-1) x min(-1)) was infused after placement of an LAD stenosis that reduced (n=8) or abolished (n=7) flow reserve. In dogs with reduced flow reserve, the stenotic-to-normal sestamibi activity ratio (0.86+/-0.03) significantly underestimated the approximately 2-to-1 dobutamine-induced flow disparity at the time of sestamibi injection (flow ratio, 0.53+/-0.04; P<.001). Stenotic-zone thickening increased at low but not at higher doses of dobutamine (2.9+/-0.4 versus 4.2+/-0.4 mm in normal zone at peak dobutamine; P=.055) but did not fall below baseline (2.7+/-0.3 mm). Similarly, in dogs with absent flow reserve, the sestamibi activity ratio (0.78+/-0.02) underestimated the approximately 2.5-to-1 dobutamine-induced flow disparity (flow ratio, 0.41+/-0.05; P<.001), and failure to increase systolic thickening was observed in the stenotic zone (2.7+/-0.4 versus 4.6+/-0.3 mm in the normal zone at peak stress, P<.01). In both groups of dogs, myocardial sestamibi uptake and image defect magnitudes were less than expected for the dobutamine-induced hyperemia, suggesting that dobutamine adversely affects myocardial sestamibi binding. Finally, a significant reduction in stenotic-zone thickening was seen during postdobutamine recovery, consistent with myocardial stunning.
  
  
  CONCLUSIONS
  In the presence of stenoses that reduced or abolished regional flow reserve, (1) myocardial sestamibi uptake significantly underestimated the dobutamine-induced flow heterogeneity, (2) a "failure to increase systolic thickening" rather than a reduction in thickening was observed during dobutamine stress, and (3) myocardial stunning was observed during postdobutamine recovery.
authors.affiliation: Department of Medicine, University of Virginia Health Sciences Center, Charlottesville 22908, USA.
authors.forename: D A
authors.initials: DA
authors.lastname: Calnon
authors.forename: D K
authors.initials: DK
authors.lastname: Glover
authors.forename: G A
authors.initials: GA
authors.lastname: Beller
authors.forename: G
authors.initials: G
authors.lastname: Vanzetto
authors.forename: W H
authors.initials: WH
authors.lastname: Smith
authors.forename: D D
authors.initials: DD
authors.lastname: Watson
authors.forename: M
authors.initials: M
authors.lastname: Ruiz
mesh_terms: D000704:Analysis of Variance; D000818:Animals; D001692:Biological Transport; D001794:Blood Pressure / Q000187:drug effects; D003326:Coronary Circulation / Q000187:drug effects* / Q000502:physiology; D003327:Coronary Disease / Q000000981:diagnostic imaging / Q000503:physiopathology*; D003331:Coronary Vessels / Q000187:drug effects / Q000503:physiopathology; D004280:Dobutamine / Q000494:pharmacology*; D004285:Dogs; D005080:Exercise Test / Q000379:methods; D006321:Heart / Q000000981:diagnostic imaging / Q000187:drug effects* / Q000503:physiopathology; D006339:Heart Rate / Q000187:drug effects; D006439:Hemodynamics / Q000187:drug effects* / Q000502:physiology; D017682:Myocardial Stunning / Q000503:physiopathology*; D009206:Myocardium / Q000378:metabolism; D011877:Radionuclide Imaging; D013599:Systole; D017256:Technetium Tc 99m Sestamibi / Q000493:pharmacokinetics*; D016277:Ventricular Function, Left / Q000187:drug effects
chemical_list: D004280:Dobutamine; D017256:Technetium Tc 99m Sestamibi