title: [Kinetics of myelopoietic regeneration and mobilization of CD34-positive cells within the scope of the NB90 Neuroblastoma Therapy Study].
abstract: Hematological and clinical data of 14 children with neuroblastoma treated according to the German neuroblastoma therapy study NB 90 were analyzed. Therapy included 4 or 8 intensive therapy elements N1 (Etoposide 125 mg/m2 day 1-4, Vindesine 3 mg/m2 day 1, Cisplatin 40 mg/m2 day 1-4) and N2 (Vincristine 1.5 mg/m2 day 1 + 8, Dacarbazine 200 mg/m2 day 1-5, Ifosfamide 1500 mg/ m2 day 1-5, Doxorubicin 30 mg/m2 day 6 + 7) in alternating order. The hematological recovery was studied after 86 therapy elements N1/N2. G-CSF had been given in 23 therapy courses, while no cytokine was administered in 63 therapy courses. Mobilization of CD34+ cells was studied in 13 therapy courses with G-CSF. Severe myelosuppression with an absolute neutrophil count < 500/microL was noted 2-4 weeks after each therapy element. The use of G-CSF did not prevent, but shortened neutropenia. There was no difference in the number of infections nor time delay of therapy between the courses with or without G-CSF. In 11 therapy courses G-CSF was started on the day following the last chemotherapy dose (N1: day 5; N2: day 9). In 12 therapy courses G-CSF was given delayed, starting day 12 after the initiation of therapy. Kinetics of granulocyte recovery was similar in the early or delayed application of G-CSF. Neutrophil recovery after the therapy element N1 was earlier and faster compared to that of therapy element N2. The more rapid rise of the neutrophils after the N1 element was accompanied by an effective mobilization of CD34+ cells. Taking into account the limitations of this retrospective study, the data may help to optimize the application of G-CSF in a very intensive therapy study like NB90.
authors.affiliation: Universitäts-Kinderklinik Freiburg.
authors.forename: S
authors.initials: S
authors.lastname: Sauter
authors.forename: J
authors.initials: J
authors.lastname: Bauer
authors.forename: R
authors.initials: R
authors.lastname: Berner
authors.forename: U
authors.initials: U
authors.lastname: Duffner
authors.forename: C
authors.initials: C
authors.lastname: Niemeyer
mesh_terms: D000293:Adolescent; D018952:Antigens, CD34 / Q000097:blood*; D000971:Antineoplastic Combined Chemotherapy Protocols / Q000627:therapeutic use; D001853:Bone Marrow / Q000473:pathology*; D016465:Bone Marrow Purging; D002648:Child; D002675:Child, Preschool; D003131:Combined Modality Therapy; D005260:Female; D016179:Granulocyte Colony-Stimulating Factor / Q000008:administration & dosage; D006098:Granulocytes / Q000276:immunology; D006801:Humans; D007223:Infant; D007958:Leukocyte Count; D008297:Male; D009447:Neuroblastoma / Q000276:immunology / Q000473:pathology / Q000628:therapy*; D009504:Neutrophils / Q000276:immunology; D012038:Regeneration / Q000502:physiology*; D012189:Retrospective Studies; D012983:Soft Tissue Neoplasms / Q000276:immunology / Q000473:pathology / Q000628:therapy*
chemical_list: D018952:Antigens, CD34; D016179:Granulocyte Colony-Stimulating Factor