[Determination of the passage of two markers in the gastrointestinal tract of steers using different evaluation methods: effect of the level of nutrition].
This study was conducted to investigate the effects of amount of intake of a mixed diet by steers on the passage of digesta through the reticulorumen and the whole digestive tract. Six ruminally cannulated steers received a mixed diet consisting on average of 43% grass silage, 25% maize silage, 30% concentrate and 2% mineral-vitamin mix in DM. The experimental design was a repeated 3 x 3 Latin square with 21 days periods. The diet was offered twice daily (07.00 and 19.00 h) at 1, 1.5 and 2 times of estimated maintenance energy requirements. At the beginning of each period, animals received pulse doses of Titanium(IV)-oxide (TiO2) per os and Cr-EDTA intraruminally. Following marker administration, faecal marker concentrations were determined over 120 h. Passage parameters were estimated by a mono-exponential and a bi-exponential model, by models including gamma age-dependency and by a model calculating total tract mean retention time as mean value of all points on the marker-excretion curve. Passage rate of TiO2 from the reticulorumen increased with higher intakes, whereas mean retention time in the whole tract decreased. In general, results of different models were consistent across intakes. Values for passage of TiO2 were in good agreement with those reported for small particles, when similar diets were fed to cattle. Rate of passage of Cr-EDTA from the reticulorumen increased with higher intakes, and mean retention times of Cr-EDTA in the reticulorumen and in the whole tract decreased. Differences between models with or without age dependency were greater for Cr-EDTA than for TiO2. Fit to Cr-EDTA excretion curves was not satisfactory for models with gamma age dependency. Irrespective of model and marker, passage from the reticulorumen accelerated markedly, whereas retention times in the reticulorumen and in the whole tract decreased, as intake increased from maintenance energy requirements to 1.5 times maintenance.
Rothfuss
H
H
Institut für Tierernährung und Stoffwechselphysiologie, Christian-Albrechts-Universität Kiel, Germany.
Südekum
K H
KH
Stangassinger
M
M
D000375:Aging / Q000378:metabolism / Q000502:physiology; D000818:Animals; D015415:Biomarkers / Q000032:analysis; D002404:Catheterization / Q000379:methods / Q000662:veterinary; D002417:Cattle / Q000378:metabolism / Q000502:physiology*; D017608:Chromium Compounds / Q000032:analysis* / Q000378:metabolism; D004032:Diet / Q000662:veterinary*; D004063:Digestion / Q000502:physiology; D004064:Digestive System / Q000737:chemistry* / Q000378:metabolism; D004068:Digestive System Physiological Phenomena; D004435:Eating / Q000502:physiology*; D004492:Edetic Acid / Q000032:analysis* / Q000378:metabolism; D005243:Feces / Q000737:chemistry; D005769:Gastrointestinal Motility / Q000502:physiology; D008297:Male; D008903:Minerals / Q000378:metabolism; D006109:Poaceae / Q000378:metabolism; D012159:Reticulum / Q000378:metabolism / Q000502:physiology; D012417:Rumen / Q000378:metabolism / Q000502:physiology; D014025:Titanium / Q000032:analysis* / Q000378:metabolism; D014815:Vitamins / Q000378:metabolism; D003313:Zea mays / Q000378:metabolism
D015415:Biomarkers; D017608:Chromium Compounds; D008903:Minerals; D014815:Vitamins; C009495:titanium dioxide; D004492:Edetic Acid; D014025:Titanium