Allele-specific PCR amplification of factor V Leiden to identify patients at risk for thromboembolism.
Resistance to activated protein C is the most common hereditary cause of thrombophilia and is significantly linked to factor V Leiden. We designed primers in order to identify factor V Leiden by allele-specific PCR amplification. Amplification specificity for factor V was ensured by a 3' primer located at the intron 9/exon 10 border of the gene. One sense and two antisense primers were used in two separate primer mixes specific for factor V ARG506 (wild-type) or factor V GLN506 (factor V Leiden). In each PCR reaction a pair of primers amplifying a fragment of the human growth hormone gene was included as an internal positive amplification control. The presence or absence of specific PCR amplification allowed definite allele assignment without the need for any postamplification specificity step. The internal positive control primers indicate a successful PCR amplification, allowing the assignment of homozygosity. In a prospective study 126 patients with thromboembolic events were analyzed using this technique and PCR-RFLP. The concordance between these methods was 100%. In 27 patients a heterozygous factor V GLN506 mutation was detected, whereas 1 patient with recurrent thromboembolism was homozygous. No false-positive or false-negative results were observed in the homozygous as well as heterozygous samples. Additionally, in 15 samples identified to carry the point mutation by allele-specific PCR amplification, automatic sequencing has confirmed the heterozygous or homozygous point mutation. Due to its time- and cost-saving features allele-specific amplification should be considered for screening of factor V Leiden.
Blasczyk
R
R
Department of Internal Medicine, Virchow-Klinikum, Humboldt-Universität zu Berlin, Germany.
Wehling
J
J
Ritter
M
M
Neubauer
A
A
Riess
H
H
D000483:Alleles; D005165:Factor V / Q000235:genetics*; D005784:Gene Amplification / Q000235:genetics*; D006580:Genetic Carrier Screening; D006720:Homozygote; D006801:Humans; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length; D013923:Thromboembolism / Q000097:blood / Q000175:diagnosis / Q000235:genetics*; D019851:Thrombophilia / Q000097:blood / Q000175:diagnosis / Q000235:genetics*
C095381:factor V Leiden; D005165:Factor V