title: Oligonucleotide dendrimers: synthesis and use as polylabelled DNA probes.
abstract: Oligonucleotide dendrimers were synthesized using a novel phosphoramidite synthon, tris-2,2,2-[3-(4,4'-dimethoxytrityloxy) propyloxymethyl]ethyl- N , N -diisopropylaminocyanethoxy phosphoramidite. Label, incorporated using [gamma-32P]ATP and polynucleotide kinase, was increased in proportion to the number of 5'-ends. There was a similar increase in signal when these multiply labelled oligonucleotides were used as probes to oligonucleotide arrays. A dendrimeric oligonucleotide was used successfully as a primer in the PCR. The strand bearing the dendrimer was resistant to degradation by T7 Gene 6 exonuclease making it easy to convert the double-stranded product of the PCR to a multiply-labelled, single-stranded probe.
authors.affiliation: Department of Biochemistry, University of Oxford, South Parks Road, Oxford OX1 3QU, UK. misha@bioch.ox.ac.uk
authors.forename: M S
authors.initials: MS
authors.lastname: Shchepinov
authors.forename: I A
authors.initials: IA
authors.lastname: Udalova
authors.forename: A J
authors.initials: AJ
authors.lastname: Bridgman
authors.forename: E M
authors.initials: EM
authors.lastname: Southern
mesh_terms: D000255:Adenosine Triphosphate; D001704:Biopolymers; D017931:DNA Primers / Q000138:chemical synthesis*; D015342:DNA Probes / Q000138:chemical synthesis*; D007553:Isotope Labeling; D009841:Oligonucleotides / Q000138:chemical synthesis*; D010761:Phosphorus Radioisotopes; D016133:Polymerase Chain Reaction; D011116:Polynucleotide 5'-Hydroxyl-Kinase
chemical_list: D001704:Biopolymers; D017931:DNA Primers; D015342:DNA Probes; D009841:Oligonucleotides; D010761:Phosphorus Radioisotopes; D000255:Adenosine Triphosphate; D011116:Polynucleotide 5'-Hydroxyl-Kinase