title: Cloning of a gene encoding a putative xylanase with a cellulose-binding domain from Humicola grisea.
abstract: We have isolated a genomic clone of a putative xylanase gene (xyn1) from Humicola grisea by using the DNA fragment encoding a cellulose-binding domain of H. grisea cellobiohydrolase 1 as a probe. The translation product of the xyn1 gene predicts a xylanase of 429 amino acids in length, with a cellulose-binding domain in the C-terminus.
authors.affiliation: Department of Biotechnology, Graduate School of Agricultural and Life Sciences, University of Tokyo, Japan.
authors.forename: H
authors.initials: H
authors.lastname: Iikura
authors.forename: S
authors.initials: S
authors.lastname: Takashima
authors.forename: A
authors.initials: A
authors.lastname: Nakamura
authors.forename: H
authors.initials: H
authors.lastname: Masaki
authors.forename: T
authors.initials: T
authors.lastname: Uozumi
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D002482:Cellulose / Q000378:metabolism; D003001:Cloning, Molecular; D003062:Codon; D015342:DNA Probes; D004271:DNA, Fungal / Q000096:biosynthesis / Q000235:genetics; D005800:Genes, Fungal / Q000235:genetics*; D003904:Mitosporic Fungi / Q000378:metabolism*; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D043325:Xylan Endo-1,3-beta-Xylosidase; D014995:Xylosidases / Q000096:biosynthesis* / Q000235:genetics
chemical_list: D003062:Codon; D015342:DNA Probes; D004271:DNA, Fungal; D002482:Cellulose; D014995:Xylosidases; D043325:Xylan Endo-1,3-beta-Xylosidase