title: Coupled formation of an amidotransferase interdomain ammonia channel and a phosphoribosyltransferase active site.
abstract: Activation of gluatmine phosphoribosylpyrophosphate (RPPP) amidotransferase (GPATase) by binding of a PRPP substrate analog results in the formation of a 20 A channel connecting the active site for glutamine hydrolysis in one domain with the PRPP site in a second domain. This solvent-inaccessible channel permits transfer of the NH3 intermediate between the two active sites. Tunneling of NH3 may be a common mechanism for glutamine amidotransferase-catalyzed nitrogen transfer and for coordination of catalysis at two distinct active sites in complex enzymes. The 2.4 A crystal structure of the active conformer of GPATase also provides the first description of an intact active site for the phosphoribosyltransferase (PRTase) family of nucleotide synthesis and salvage enzymes. Chemical assistance to catalysis is provided primarily by the substrate and secondarily by the enzyme in the proposed structure-based mechanism. Different catalytic and inhibitory modes of divalent cation binding to the PRTase active site are revealed in the active conformer of the enzyme and in a feedback-inhibited GMP complex.
authors.affiliation: Department of Biological Sciences, Purdue University, West Lafayette, Indiana 47907, USA.
authors.forename: J M
authors.initials: JM
authors.lastname: Krahn
authors.forename: J H
authors.initials: JH
authors.lastname: Kim
authors.forename: M R
authors.initials: MR
authors.lastname: Burns
authors.forename: R J
authors.initials: RJ
authors.lastname: Parry
authors.forename: H
authors.initials: H
authors.lastname: Zalkin
authors.forename: J L
authors.initials: JL
authors.lastname: Smith
mesh_terms: D000582:Amidophosphoribosyltransferase / Q000737:chemistry / Q000378:metabolism*; D000641:Ammonia / Q000378:metabolism*; D001665:Binding Sites; D018360:Crystallography, X-Ray; D008956:Models, Chemical; D008958:Models, Molecular; D008969:Molecular Sequence Data; D010754:Phosphoribosyl Pyrophosphate / Q000378:metabolism*; D011487:Protein Conformation
chemical_list: D010754:Phosphoribosyl Pyrophosphate; D000641:Ammonia; D000582:Amidophosphoribosyltransferase