Pseudomonas aeruginosa infection in embryonated hen's eggs. An alternative in vivo model for the screening of antibacterial substances.
Embryonated hens' eggs can be reliably infected by Pseudomonas aeruginosa in laboratory experiments. Therapy tests with the antibiotics azlocillin (CAS 37091-66-0) and gentamicin (CAS 13291-74-2) on this type of infected hens' eggs demonstrate that this test system offers a realistic alternative to septic experiments with small laboratory rodents. Chick embryos survive a lethal Pseudomonas infection when azlocillin or gentamicin in a relevant therapeutic dose are administered immediately after the infective agent. The use of Pseudomonas infected chick embryos in the screening for new antiinfectives allows, therefore, a considerable reduction of the number of laboratory rodents required.
Härtl
A
A
Hans-Knöll-Institute of Natural Product Research e.V., Jena, Germany.
Möllmann
U
U
Schrinner
E
E
Stelzner
A
A
D000826:Animal Testing Alternatives; D000818:Animals; D000900:Anti-Bacterial Agents / Q000627:therapeutic use*; D001390:Azlocillin / Q000627:therapeutic use; D002642:Chick Embryo / Q000382:microbiology*; D004353:Drug Evaluation, Preclinical; D005839:Gentamicins / Q000627:therapeutic use; D011552:Pseudomonas Infections / Q000188:drug therapy* / Q000382:microbiology
D000900:Anti-Bacterial Agents; D005839:Gentamicins; D001390:Azlocillin