Rapid desilylation of oligoribonucleotides at elevated temperatures: cleavage activity in ribozyme-substrate assays.
Treatment of 2'-O-silyl-oligoribonucleotides with triethylamine trihydrofluoride in DMF at 55 degrees C for 1 h effected complete desilylation. The product was isolated by a single addition of 1-butanol to the reaction mixture. The resulting RNA was found to be identical with that obtained by traditional desilylation methods as analyzed by HPLC, enzyme digest and ribozyme-substrate assays.
Vinayak
R
R
Division of Perkin Elmer Corporation, Foster City, CA 94404, USA.
Andrus
A
A
Hampel
A
A
D001483:Base Sequence; D001665:Binding Sites; D002851:Chromatography, High Pressure Liquid; D006678:HIV / Q000378:metabolism; D007202:Indicators and Reagents; D007700:Kinetics; D008969:Molecular Sequence Data; D009843:Oligoribonucleotides / Q000138:chemical synthesis / Q000737:chemistry*; D016337:RNA, Catalytic / Q000737:chemistry* / Q000378:metabolism*; D012821:Silanes; D013816:Thermodynamics
D007202:Indicators and Reagents; D009843:Oligoribonucleotides; D016337:RNA, Catalytic; D012821:Silanes