High pressure modulation of Escherichia coli DNA gyrase activity.
Elevated pressures greater than 551 bar were found to inhibit the DNA supercoiling activity of Escherichia coli DNA gyrase. A large fraction of the inhibitory effect could be reproduced by preincubation of the enzyme at high pressure prior to enzymatic assay at 1 bar. It is proposed that elevated pressure influences gyrase structure, most likely by promoting the dissociation of its subunits, however, it is also possible that effects on enzyme activity exist.
Chilukuri
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LN
Center for Marine Biotechnology and Biomedicine, Scripps Institution of Oceanography, La Jolla, California 92093, USA.
Fortes
P A
PA
Bartlett
D H
DH
D001274:Atmospheric Pressure; D004250:DNA Topoisomerases, Type II / Q000378:metabolism*; D004278:DNA, Superhelical / Q000378:metabolism; D004587:Electrophoresis, Agar Gel; D004789:Enzyme Activation; D004926:Escherichia coli / Q000201:enzymology*; D010957:Plasmids / Q000378:metabolism; D013379:Substrate Specificity
D004278:DNA, Superhelical; D004250:DNA Topoisomerases, Type II