CTL responses induced by a single immunization with peptide encapsulated in biodegradable microparticles.
A synthetic peptide representing a measles virus (MV) cytotoxic T cell epitope (CTL) when encapsulated in poly (D,L-lactide co-glycolide) (PLG) 50:50 microparticles induced a strong CTL response after a single intraperitoneal immunization of mice which was greater than that following administration of the peptide in Freund's complete adjuvant. A 100 micrograms dose of encapsulated peptide was shown to be more effective for CTL priming than 50 and 25 micrograms doses. A vaccine formulation prepared by simply mixing empty 50:50 PLG microparticles with the peptide resulted in the induction of CTL responses comparable to those induced by the encapsulated peptide. Moreover, a CTL response against MV-infected target cells was observed. These findings highlight the potential immunostimulatory effect of PLG microparticles for the induction of MV and peptide-specific CTL responses.
Partidos
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Department of Pathology and Infectious Diseases, Royal Veterinary College, London, UK. hpartido@rvc.ac.uk
Vohra
P
P
Jones
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D
Farrar
G
G
Steward
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MW
D000595:Amino Acid Sequence; D000818:Animals; D017951:Antigen Presentation; D002352:Carrier Proteins / Q000008:administration & dosage / Q000276:immunology*; D004306:Dose-Response Relationship, Immunologic; D000939:Epitopes / Q000008:administration & dosage / Q000276:immunology; D019344:Lactic Acid / Q000008:administration & dosage*; D008459:Measles virus / Q000276:immunology; D051379:Mice; D008805:Mice, Inbred A; D008808:Mice, Inbred CBA; D008863:Microspheres; D008969:Molecular Sequence Data; D009447:Neuroblastoma; D019590:Nucleocapsid Proteins; D009698:Nucleoproteins / Q000008:administration & dosage / Q000276:immunology; D010446:Peptide Fragments / Q000008:administration & dosage / Q000276:immunology*; D011100:Polyglycolic Acid / Q000008:administration & dosage*; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011108:Polymers / Q000008:administration & dosage*; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology*; D014407:Tumor Cells, Cultured; D014611:Vaccination; D014764:Viral Proteins / Q000008:administration & dosage / Q000276:immunology
D002352:Carrier Proteins; D000939:Epitopes; D019590:Nucleocapsid Proteins; D009698:Nucleoproteins; D010446:Peptide Fragments; D011108:Polymers; D014764:Viral Proteins; C058314:nucleoprotein, Measles virus; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011100:Polyglycolic Acid; D019344:Lactic Acid