Effect of cyclosporin A on tissue lipid peroxidation and membrane bound phosphatases in hyperoxaluric rat and the protection by vitamin E pretreatment.
The effect of cyclosporin A, a highly effective immunosuppressant, was investigated on hyperoxaluric rats with and without vitamin E pretreatment. Hyperoxaluria was induced by oral feeding of 3% ammonium oxalate in water for 3 days. Cyclosporin A (50 mg/kg body wt.) was administered for 3 days. Pretreatment with vitamin E (50 mg/100 g body wt., once a week for 3 weeks) was carried out before the administration of cyclosporin A and ammonium oxalate. Nonenzymatic ascorbate-induced lipid peroxidation was increased to 1.55-fold in either cyclosporin A-administered or hyperoxaluric rat kidney and liver when compared to control. The lipid peroxidation was further elevated to 1.9-fold when both cyclosporin A and ammonium oxalate were coadministered. The activities of renal and hepatic ATPase, glucose-6-phosphatase as well as the concentrations of thiols were decreased significantly (p < 0.001) when cyclosporin A was administered under hyperoxaluric condition. On pretreatment with vitamin E the cyclosporin A-induced biochemical changes observed in the presence of hyperoxaluria were abolished.
Adhirai
M
M
Department of Biochemistry, Dr. A.L.M. PG Institute of Basic Medical Sciences, University of Madras, Taramani, India.
Selvam
R
R
D000818:Animals; D002462:Cell Membrane / Q000187:drug effects / Q000201:enzymology; D016572:Cyclosporine / Q000494:pharmacology*; D006959:Hyperoxaluria / Q000188:drug therapy* / Q000201:enzymology; D007166:Immunosuppressive Agents / Q000494:pharmacology*; D015227:Lipid Peroxidation / Q000187:drug effects*; D008297:Male; D010744:Phosphoric Monoester Hydrolases / Q000187:drug effects* / Q000378:metabolism; D011292:Premedication; D051381:Rats; D017208:Rats, Wistar; D014810:Vitamin E / Q000627:therapeutic use*
D007166:Immunosuppressive Agents; D014810:Vitamin E; D016572:Cyclosporine; D010744:Phosphoric Monoester Hydrolases