Comparison of exopolyphosphatases of different yeast cell compartments.
Purified cell-envelope polyphosphatase as well as polyphoshatase activities of cytosol and isolated vacuoles, of nuclei and mitochondria of the yeast Saccharomyces cerevisiae were compared. The polyphosphatases of cell envelope and cytosol are similar, the polyphosphatases of nuclei, vacuoles and mitochondria differ in their kinetic properties, substrate specificity, requirements in divalent cations and in some effector actions both from these and from each other.
Kulaev
I S
IS
Institute of Biochemistry and Physiology of Microorganisms, Russian Academy of Sciences, Pushchino, Russia.
Andreeva
N A
NA
Lichko
L P
LP
Kulakovskaya
T V
TV
D017766:Acid Anhydride Hydrolases / Q000378:metabolism*; D002467:Cell Nucleus / Q000201:enzymology; D003600:Cytosol / Q000201:enzymology; D004492:Edetic Acid / Q000494:pharmacology; D007118:Immunoassay; D008928:Mitochondria / Q000201:enzymology; D012441:Saccharomyces cerevisiae / Q000201:enzymology* / Q000648:ultrastructure; D013379:Substrate Specificity; D014617:Vacuoles / Q000201:enzymology
D004492:Edetic Acid; D017766:Acid Anhydride Hydrolases; C022563:endopolyphosphatase