title: Molecular cloning and expression of a rat cDNA encoding 5-aminoimidazole-4-carboxamide ribonucleotide formyltransferase/IMP cyclohydrolase.
abstract: The cDNA of a 5-aminoimidazole-4-carboxamide ribonucleotide formyltransferase/IMP cyclohydrolase (AICARFT/IMPCHase) was isolated from rat liver RNA by reverse transcription and the polymerase chain reaction (PCR). The rat AICARFT/IMPCHase cDNA included 1928 bp containing a coding region of 1779 bp for a 592-amino acid polypeptide (Mr = 64 200). Rat and human AICARFT/IMPCHase cDNAs show 84 and 91% homology at the nucleotide and amino acid sequence level, respectively. The protein produced by the rat cDNA using pET-expression system catalysed the penultimate and final steps of de novo purine biosynthesis. Northern analysis identified a 2.8-kb AICARFT/IMPCHase mRNA and the level of the AICARFT/IMPCHase transcripts increased markedly at 24 h after partial (70%) hepatectomy.
authors.affiliation: Department of Food Science and Nutrition, Nara Women's University, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Akira
authors.forename: M
authors.initials: M
authors.lastname: Komatsu
authors.forename: R
authors.initials: R
authors.lastname: Nango
authors.forename: A
authors.initials: A
authors.lastname: Tomooka
authors.forename: K
authors.initials: K
authors.lastname: Konaka
authors.forename: M
authors.initials: M
authors.lastname: Yamauchi
authors.forename: Y
authors.initials: Y
authors.lastname: Kitamura
authors.forename: S
authors.initials: S
authors.lastname: Nomura
authors.forename: I
authors.initials: I
authors.lastname: Tsukamoto
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics*; D004926:Escherichia coli / Q000235:genetics; D015971:Gene Expression Regulation, Enzymologic / Q000235:genetics*; D006498:Hepatectomy; D019877:Hydroxymethyl and Formyl Transferases / Q000235:genetics*; D008099:Liver / Q000502:physiology; D008115:Liver Regeneration / Q000235:genetics; D008969:Molecular Sequence Data; D009709:Nucleotide Deaminases / Q000235:genetics*; D051027:Phosphoribosylaminoimidazolecarboxamide Formyltransferase; D012333:RNA, Messenger / Q000032:analysis; D051381:Rats; D011994:Recombinant Proteins; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D012689:Sequence Homology, Nucleic Acid
chemical_list: D018076:DNA, Complementary; D012333:RNA, Messenger; D011994:Recombinant Proteins; D019877:Hydroxymethyl and Formyl Transferases; D051027:Phosphoribosylaminoimidazolecarboxamide Formyltransferase; D009709:Nucleotide Deaminases; C021436:IMP cyclohydrolase