title: Detection of nucleoprotein gene of Sendai virus in the lungs of rats by touchdown nested reverse transcription polymerase chain reaction.
abstract: The nucleoprotein (NP) gene of Sendai virus was detected by touchdown nested reverse transcription polymerase chain reaction (RT-PCR) in the lungs of a rat presented with respiratory illness and high serum ELISA titer to Sendai virus. This method seemed to be of value in controlling infection in laboratory rodents.
authors.affiliation: Department of Virology, Jichi Medical School, Tochigi, Japan.
authors.forename: Y
authors.initials: Y
authors.lastname: Hayase
authors.forename: K
authors.initials: K
authors.lastname: Tobita
authors.forename: M
authors.initials: M
authors.lastname: Kii
authors.forename: Y
authors.initials: Y
authors.lastname: Hakamada
authors.forename: T
authors.initials: T
authors.lastname: Arai
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D000830:Animals, Laboratory; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D005814:Genes, Viral / Q000235:genetics*; D008171:Lung Diseases / Q000175:diagnosis; D008969:Molecular Sequence Data; D009698:Nucleoproteins / Q000235:genetics*; D016133:Polymerase Chain Reaction; D051381:Rats; D018108:Respirovirus / Q000235:genetics*; D010253:Respirovirus Infections / Q000175:diagnosis* / Q000235:genetics
chemical_list: D009698:Nucleoproteins