title: DSC studies of the conformational stability of barstar wild-type.
abstract: The temperature induced unfolding of barstar wild-type of bacillus amyloliquefaciens (90 residues) has been characterized by differential scanning microcalorimetry. The process has been found to be reversible in the pH range from 6.4 to 8.3 in the absence of oxygen. It has been clearly shown by a ratio of delta HvH/delta Hcal near 1 that denaturation follows a two-state mechanism. For comparison, the C82A mutant was also studied. This mutant exhibits similar reversibility, but has a slightly lower transition temperature. The transition enthalpy of barstar wt (303 kJ mol-1) exceeds that of the C82A mutant (276 kJ mol-1) by approximately 10%. The heat capacity changes show a similar difference, delta Cp being 5.3 +/- 1 kJ mol-1 K-1 for the wild-type and 3.6 +/- 1 kJ mol-1 K-1 for the C82A mutant. The extrapolated stability parameters at 25 degrees C are delta G0 = 23.5 +/- 2 kJ mol-1 for barstar wt and delta G0 = 25.5 +/- 2 kJ mol-1 for the C82A mutant.
authors.affiliation: Institut für Physikalische Chemie, Westfälischen Wilhelms-Universität Münster, Germany.
authors.forename: A
authors.initials: A
authors.lastname: Schöppe
authors.forename: H J
authors.initials: HJ
authors.lastname: Hinz
authors.forename: V R
authors.initials: VR
authors.lastname: Agashe
authors.forename: S
authors.initials: S
authors.lastname: Ramachandran
authors.forename: J B
authors.initials: JB
authors.lastname: Udgaonkar
mesh_terms: D001407:Bacillus / Q000737:chemistry*; D001426:Bacterial Proteins / Q000737:chemistry*; D002152:Calorimetry, Differential Scanning; D004229:Dithiothreitol / Q000494:pharmacology; D004355:Drug Stability; D004791:Enzyme Inhibitors / Q000737:chemistry*; D006358:Hot Temperature; D006863:Hydrogen-Ion Concentration; D008958:Models, Molecular; D009154:Mutation; D011487:Protein Conformation; D011489:Protein Denaturation; D012260:Ribonucleases / Q000037:antagonists & inhibitors; D013439:Sulfhydryl Reagents / Q000494:pharmacology; D013816:Thermodynamics
chemical_list: D001426:Bacterial Proteins; D004791:Enzyme Inhibitors; D013439:Sulfhydryl Reagents; C057438:barstar protein, Bacillus amyloliquefaciens; D012260:Ribonucleases; C019760:Bacillus amyloliquefaciens ribonuclease; D004229:Dithiothreitol