Fast blue, a fluorescent tracer in glioma cell culture, affects cell proliferation and motility.
The azo-dye, Fast Blue (FB), initially employed for retrograde neuronal tracing is increasingly used in cell invasion and migration studies to detect living cells in monolayer and glioma tumor cell spheroid models. As yet, it is assumed that a cell stained with a tracker dye retains the characteristics of the original cell. The following experiments compared the adhesion, migration and proliferation properties of the cell lines U373 and GaMG with and without FB staining. FB staining reduced cell adhesion (P < 0.01) and proliferative activity (P < 0.01) and also had a significant inhibitory effect on cell migration (P < 0.001). From the results presented it follows that FB staining markedly influences basic cell characteristics.
Vince
G H
GH
Department of Neurosurgery, University of Würzburg, Germany. nch-tumorbiology@mail.uni-wuerzburg.de
Bouterfa
H
H
Goldbrunner
R
R
Roosen
K
K
Tonn
J C
JC
D000578:Amidines; D002448:Cell Adhesion / Q000187:drug effects; D002455:Cell Division / Q000187:drug effects; D002465:Cell Movement / Q000187:drug effects; D005456:Fluorescent Dyes; D005910:Glioma / Q000473:pathology*; D006801:Humans; D009361:Neoplasm Invasiveness; D014407:Tumor Cells, Cultured
D000578:Amidines; D005456:Fluorescent Dyes; C031455:diamidino compound 253-50