title: Inhibition of collagenases from mouse lung carcinoma cells by green tea catechins and black tea theaflavins.
abstract: Theaflavin and theaflavin digallate, which are components of black tea were examined by in vitro invasion assay with mouse Lewis lung carcinoma LL2-Lu3 cells, which are highly metastatic. The compounds inhibited invasion by the tumor cells. Gelatin zymography showed that the cells secreted matrix metalloproteinases (MMPs), probably including MMP-2 and MMP-9, which may be involved in tumor cell invasion and metastasis. Theaflavin and theaflavin digallate also inhibited MMPs from the culture medium of these tumor cells, as did (-)-epigallocatechin gallate. These results suggest that theaflavin, theaflavin digallate, and (-)-epigallocatechin gallate inhibit tumor cell invasion by inhibiting type IV collagenases of the LL2-Lu3 cells.
authors.affiliation: School of Food and Nutritional Sciences, University of Shizuoka, Japan.
authors.forename: M
authors.initials: M
authors.lastname: Sazuka
authors.forename: H
authors.initials: H
authors.lastname: Imazawa
authors.forename: Y
authors.initials: Y
authors.lastname: Shoji
authors.forename: T
authors.initials: T
authors.lastname: Mita
authors.forename: Y
authors.initials: Y
authors.lastname: Hara
authors.forename: M
authors.initials: M
authors.lastname: Isemura
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000494:pharmacology*; D044946:Biflavonoids; D002277:Carcinoma / Q000201:enzymology*; D002392:Catechin / Q000031:analogs & derivatives / Q000494:pharmacology*; D016166:Free Radical Scavengers / Q000494:pharmacology*; D005707:Gallic Acid / Q000031:analogs & derivatives / Q000494:pharmacology; D008175:Lung Neoplasms / Q000201:enzymology*; D061965:Matrix Metalloproteinase Inhibitors; D051379:Mice; D013662:Tea / Q000737:chemistry*; D014407:Tumor Cells, Cultured
chemical_list: D000975:Antioxidants; D044946:Biflavonoids; D016166:Free Radical Scavengers; D061965:Matrix Metalloproteinase Inhibitors; D013662:Tea; C056068:theaflavin; C077197:theaflavin digallate; D005707:Gallic Acid; D002392:Catechin; C045651:epigallocatechin gallate