Rapid pulsed-field gel electrophoresis protocol for typing of Escherichia coli O157:H7 and other gram-negative organisms in 1 day.
Genomic DNA patterns generated by pulsed-field gel electrophoresis are highly specific for different strains of an organism and have significant value in epidemiologic investigations of infectious-disease outbreaks. Unfortunately, time-consuming and tedious specimen processing is an inherent problem which limits the use of this powerful technology as a real-time epidemic investigational tool. Here, I describe a rapid method to improve the response time and provide specific bacterial strain identification for the typing of Escherichia coli O157:H7 and other gram-negative organisms in a single day.
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R K
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Washington State Department of Health, Public Health Laboratories, Seattle 98155, USA.
D004269:DNA, Bacterial / Q000032:analysis; D004196:Disease Outbreaks / Q000145:classification; D004587:Electrophoresis, Agar Gel / Q000379:methods; D016521:Electrophoresis, Gel, Pulsed-Field / Q000379:methods; D004927:Escherichia coli Infections / Q000175:diagnosis* / Q000453:epidemiology; D019453:Escherichia coli O157 / Q000145:classification*; D006090:Gram-Negative Bacteria / Q000145:classification*; D016905:Gram-Negative Bacterial Infections / Q000175:diagnosis* / Q000453:epidemiology; D006801:Humans; D015203:Reproducibility of Results; D012703:Serotyping / Q000379:methods*; D013997:Time Factors
D004269:DNA, Bacterial