title: Orientation of photosynthetic reaction center reconstituted in neutral and charged liposomes.
abstract: The photosynthetic reaction center from the photosynthetic bacterium Rhodobacter sphaeroides was reconstituted into neutral, positively charged, or negatively charged liposomes. About 70% of photosynthetic reaction centers were reconstituted in the proteoliposomes exposing their H-subunit outside with positively charged lipids while only 30-40% of them were in the same topological orientation with neutral or negatively charged lipids.
authors.affiliation: National Institute of Bioscience and Human-Technology, AIST, MITI, Ibaraki, Japan.
authors.forename: M
authors.initials: M
authors.lastname: Hara
authors.forename: T
authors.initials: T
authors.lastname: Ueno
authors.forename: T
authors.initials: T
authors.lastname: Fujii
authors.forename: Q
authors.initials: Q
authors.lastname: Yang
authors.forename: Y
authors.initials: Y
authors.lastname: Asada
authors.forename: J
authors.initials: J
authors.lastname: Miyake
mesh_terms: D003574:Cytochrome c Group / Q000378:metabolism; D004579:Electron Transport; D050356:Lipid Metabolism; D008055:Lipids / Q000737:chemistry; D008081:Liposomes / Q000737:chemistry*; D008567:Membranes, Artificial; D010316:Particle Size; D010788:Photosynthesis / Q000502:physiology*; D012242:Rhodobacter sphaeroides / Q000737:chemistry / Q000378:metabolism*
chemical_list: D003574:Cytochrome c Group; D008055:Lipids; D008081:Liposomes; D008567:Membranes, Artificial