title: Sequential assignments and secondary structure of the RNA-binding transcriptional regulator NusB.
abstract: The NusB protein is involved in transcriptional regulation in bacteriophage lambda. NusB binds to the RNA form of the nut site and along with N, NusA, NusE and NusG, stabilizes the RNA polymerase transcription complex and allows stable, persistent antitermination. NusB contains a 10 residue Arg-rich RNA-binding motif (ARM) at the N-terminus but is not sequentially homologous to any other proteins. In contrast to other known ARM-containing proteins, NusB forms a stable structure in solution in the absence of RNA. NMR spectroscopy was used to determine that NusB contains six alpha-helices: R10-Q21, 127-F34, V45-L65, Q79-S93, Y100-F114 and D118-L127. The structure of NusB makes it a member of a newly emerging class of alpha-helical RNA-binding proteins.
authors.affiliation: Macromolecular NMR Section, ABL-Basic Research Program, NCI-Frederick Cancer Research and Development Center, MD 21702, USA. http://www-brp.ncifcrf.gov/abl/msl/byrd.html.
authors.forename: A S
authors.initials: AS
authors.lastname: Altieri
authors.forename: M J
authors.initials: MJ
authors.lastname: Mazzulla
authors.forename: H
authors.initials: H
authors.lastname: Zhou
authors.forename: N
authors.initials: N
authors.lastname: Costantino
authors.forename: D L
authors.initials: DL
authors.lastname: Court
authors.forename: R A
authors.initials: RA
authors.lastname: Byrd
mesh_terms: D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000737:chemistry*; D002942:Circular Dichroism; D029968:Escherichia coli Proteins; D009682:Magnetic Resonance Spectroscopy; D008969:Molecular Sequence Data; D017510:Protein Folding; D017433:Protein Structure, Secondary; D016601:RNA-Binding Proteins / Q000737:chemistry*; D011994:Recombinant Proteins / Q000737:chemistry; D014157:Transcription Factors / Q000737:chemistry*
chemical_list: D001426:Bacterial Proteins; D029968:Escherichia coli Proteins; C074952:NusB protein, E coli; D016601:RNA-Binding Proteins; D011994:Recombinant Proteins; D014157:Transcription Factors