title: Cytokine profile induced by a primary infection with Ostertagia ostertagi in cattle.
abstract: Changes that occur in the local draining lymph nodes including, changes in cell surface markers and cytokine gene expression were studied over the first 4 weeks of a primary, Ostertagia ostertagi infection of the abomasum. Cells recovered from the abomasal lymph nodes (ABLN) after infection showed a decrease in the percentage of CD3+ cells, and an increase in the percentage of IgM+ cells and cells bearing the TcR1 marker. These changes were coincident with an increase in the proportion of activated cells (II-2R). Analysis of mitogen-stimulated ABLN cells by RNase protection assay (RPA) showed a dramatic reduction in IL-2 and IFN-gamma transcription after infection. In addition, analysis of unstimulated ABLN cells by competitive RT-PCR showed a similar decrease in demonstrable levels of IL-2 mRNA, but IL-10, IL-4 and IFN-gamma mRNA levels were elevated.
authors.affiliation: USDA, ARS, LPSI, Immunology and Disease Resistance Laboratory, Beltsville, MD 20705, USA.
authors.forename: A
authors.initials: A
authors.lastname: Canals
authors.forename: D S
authors.initials: DS
authors.lastname: Zarlenga
authors.forename: S
authors.initials: S
authors.lastname: Almeria
authors.forename: L C
authors.initials: LC
authors.lastname: Gasbarre
mesh_terms: D000018:Abomasum / Q000276:immunology / Q000473:pathology; D000818:Animals; D001483:Base Sequence; D002417:Cattle; D002418:Cattle Diseases / Q000235:genetics / Q000276:immunology* / Q000473:pathology; D016207:Cytokines / Q000096:biosynthesis* / Q000235:genetics; D017931:DNA Primers / Q000235:genetics; D007371:Interferon-gamma / Q000096:biosynthesis / Q000235:genetics; D007376:Interleukin-2 / Q000096:biosynthesis / Q000235:genetics; D008198:Lymph Nodes / Q000276:immunology / Q000473:pathology; D016131:Lymphocyte Subsets / Q000276:immunology; D008297:Male; D010029:Ostertagiasis / Q000276:immunology / Q000473:pathology / Q000662:veterinary*; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000235:genetics; D013997:Time Factors
chemical_list: D016207:Cytokines; D017931:DNA Primers; D007376:Interleukin-2; D012333:RNA, Messenger; D007371:Interferon-gamma