title: DNA damage in leukocytes and buccal and nasal epithelial cells of individuals exposed to air pollution in Mexico City.
abstract: There is an increased interest in using biological markers to monitor individuals for possible exposure to environmental toxicants. Test systems which permit the sensitive detection of DNA damage and DNA repair are critically important in such studies. The single cell gel electrophoresis (SCG) assay is a rapid and a sensitive method for the evaluation of DNA damage at the single cell level, providing information on the occurrence of DNA single-strand breaks and alkali labile sites using alkaline conditions. In this study, the differences in the basal level of DNA damage between young adults from the south (exposed principally to high levels of ozone) and young adults from the north (exposed principally to hydrocarbons and particles) of Mexico City were investigated by the SCG assay using three different cell types (leukocytes and nasal and buccal epithelial cells). We found an increased DNA migration in blood leukocytes and nasal cells from individuals who live in the southern part of the city compared to those living in the northern part; however, no differences were observed for buccal epithelial cells. These results show the feasability of using the SCG assay to evaluate DNA damage in different tissues and its great potential for use in the monitoring of humans potentially exposed to genotoxic pollutants.
authors.affiliation: Departamento de Genética y Toxicología Ambiental, Institutto de Investigaciones Biomédicas, UNAM, México D.F., México.
authors.forename: M
authors.initials: M
authors.lastname: Valverde
authors.forename: M
authors.initials: M
authors.lastname: del Carmen López
authors.forename: I
authors.initials: I
authors.lastname: López
authors.forename: I
authors.initials: I
authors.lastname: Sánchez
authors.forename: T I
authors.initials: TI
authors.lastname: Fortoul
authors.forename: P
authors.initials: P
authors.lastname: Ostrosky-Wegman
authors.forename: E
authors.initials: E
authors.lastname: Rojas
mesh_terms: D000328:Adult; D000393:Air Pollutants / Q000633:toxicity*; D002610:Cheek; D004249:DNA Damage / Q000187:drug effects*; D004848:Epithelium / Q000187:drug effects; D005260:Female; D005821:Genetic Techniques; D006304:Health Status; D006801:Humans; D007962:Leukocytes / Q000187:drug effects*; D008297:Male; D008800:Mexico; D009297:Nasal Mucosa / Q000187:drug effects*; D010126:Ozone / Q000009:adverse effects
chemical_list: D000393:Air Pollutants; D010126:Ozone