title: CD34 counts to predict the adequate collection of peripheral blood progenitor cells.
abstract: An essential prerequisite for successful procurement of sufficient autologous peripheral blood progenitor cells (PBPC) for engraftment is the optimal timing of collection. A number of surrogate markers of peripheral blood progenitor cells were analysed to identify a single test which could predict the optimum time to harvest, providing at least 2 x 10(6) CD34+ cells/kg patient body weight. The study comprised 95 patients undergoing varied mobilisation regimens with chemotherapy and G-CSF for both solid tumours and haematological malignancies. One hundred and fifty-seven PBPC harvests were collected. Full blood counts (FBC) and CD34+ cell enumeration was performed on blood samples taken during the mobilisation period and immediately prior to leucapheresis (pre-harvest). All PBPC collections were assayed for colony-forming cells and CD34+ cells in addition to a FBC. The white cell count on the day of harvest showed only weak correlation with the total number of CD34+ cells in the collection (r = 0.30). In contrast, the absolute number of circulating CD34+ cells strongly correlated with the CD34+ cell and CFU-GM yield of the corresponding apheresis product. Provided the mobilisation sample contained > or =20 x 10(6) CD34+ cells/ml, 94% of single collections, performed the following day, contained > or =2 x 10(6) CD34+ cells/kg.
authors.affiliation: National Blood Service, North London Centre, UK.
authors.forename: S
authors.initials: S
authors.lastname: Armitage
authors.forename: R
authors.initials: R
authors.lastname: Hargreaves
authors.forename: D
authors.initials: D
authors.lastname: Samson
authors.forename: M
authors.initials: M
authors.lastname: Brennan
authors.forename: E
authors.initials: E
authors.lastname: Kanfer
authors.forename: C
authors.initials: C
authors.lastname: Navarrete
mesh_terms: D000293:Adolescent; D000328:Adult; D018952:Antigens, CD34; D001772:Blood Cell Count; D005260:Female; D019650:Hematopoietic Stem Cell Mobilization / Q000379:methods*; D018380:Hematopoietic Stem Cell Transplantation; D006801:Humans; D008297:Male; D011237:Predictive Value of Tests
chemical_list: D018952:Antigens, CD34