cis-Acting sequences required for light-responsive expression of the psbDII gene in Synechococcus sp. strain PCC 7942.
We analyzed the sequences required for promoter activity and high-light responsiveness of the psbDII gene in the cyanobacterium Synechococcus sp. strain PCC 7942 by using transcriptional fusions to a lacZ reporter gene. The basal promoter drives high constitutive expression, although no canonical -35 element is evident. The smallest fragment that showed clear light-responsive expression extends from -38 to +160, which includes 52 bp of the psbDII open reading frame. Sequences downstream from the promoter, within the untranslated leader region from +11 to +24, were required for high-light induction.
Anandan
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Department of Biology, Texas A&M University, College Station 77843-3258, USA.
Golden
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D000595:Amino Acid Sequence; D000458:Cyanobacteria / Q000235:genetics* / Q000528:radiation effects*; D004252:DNA Mutational Analysis; D004307:Dose-Response Relationship, Radiation; D015964:Gene Expression Regulation, Bacterial; D005798:Genes, Bacterial; D008027:Light; D008969:Molecular Sequence Data; D016297:Mutagenesis, Site-Directed; D045322:Photosynthetic Reaction Center Complex Proteins / Q000096:biosynthesis / Q000235:genetics*; D014176:Protein Biosynthesis; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis; D012045:Regulatory Sequences, Nucleic Acid; D017384:Sequence Deletion
D045322:Photosynthetic Reaction Center Complex Proteins; D011993:Recombinant Fusion Proteins