Study on enzyme electrode biosensor of choline.
Choline oxidase was immobilized at a hydrogen peroxide electrode and the enzyme electrode was used for the amperometric determination of choline. The linear range is 0-200 mg/L with a response time of 40 seconds and a 25-microliter sample injection. The relative standard deviation (RSD) is less than 1.5% in 20 assays. The enzyme membrane can be used continuously at 25 degrees C for 60 days. The recovery rate of this method is 100.3-102.3%.
Feng
D
D
Shandong Institute of Biology, Jinan, China.
Sun
S
S
Zhu
F
F
Zhu
S
S
Wang
N
N
Ge
S
S
D000429:Alcohol Oxidoreductases / Q000378:metabolism; D015374:Biosensing Techniques; D002794:Choline / Q000032:analysis*; D004566:Electrodes; D006861:Hydrogen Peroxide / Q000737:chemistry; D006863:Hydrogen-Ion Concentration; D016014:Linear Models; D010710:Phosphates / Q000737:chemistry; D012965:Sodium Chloride / Q000737:chemistry; D013379:Substrate Specificity
D010710:Phosphates; D012965:Sodium Chloride; D006861:Hydrogen Peroxide; D000429:Alcohol Oxidoreductases; C019888:choline oxidase; D002794:Choline; C018279:sodium phosphate