title: Effect of delayed facial-facial nerve suture on facial nerve regeneration. A horseradish peroxidase tracing study in the rat.
abstract: In clinical practice, the lesioned facial nerve is usually restored by facial-facial nerve anastomosis (FFA) with some delay. The optimal time-point for facial nerve reconstruction is still unknown. This study, using rats, compared the effects of immediate and delayed FFA, i.e. FFA 7-56 days after interruption of the facial nerve. Muscle reinnervation was studied 42 days after nerve suture by counting all retrogradely labelled facial motoneurons after injection of horseradish peroxidase (HRP) into the whiskerpad of the rats. Immediate FFA caused a local hyperinnervation of the target muscle, i.e. the projection of more neurons into the whiskerpad muscles than under normal conditions. FFA delayed for 7 days resulted in a significant suppression of this hyperinnervation, whereas longer delay times of 10-56 days showed no difference from immediate FFA.
authors.affiliation: Department of Otorhinolaryngology, University of Cologne, Germany. orlando.guntinas@uni-koeln.de
authors.forename: O
authors.initials: O
authors.lastname: Guntinas-Lichius
authors.forename: M
authors.initials: M
authors.lastname: Streppel
authors.forename: D N
authors.initials: DN
authors.lastname: Angelov
authors.forename: E
authors.initials: E
authors.lastname: Stennert
authors.forename: W F
authors.initials: WF
authors.lastname: Neiss
mesh_terms: D000704:Analysis of Variance; D000714:Anastomosis, Surgical / Q000379:methods; D000818:Animals; D004576:Electromyography; D005152:Facial Muscles / Q000294:innervation; D005154:Facial Nerve / Q000502:physiology / Q000601:surgery*; D005260:Female; D006735:Horseradish Peroxidase; D009416:Nerve Regeneration; D051381:Rats; D017208:Rats, Wistar; D011930:Reaction Time; D013997:Time Factors
chemical_list: D006735:Horseradish Peroxidase