title: The fission yeast protein p73res2 is an essential component of the mitotic MBF complex and a master regulator of meiosis.
abstract: Depending on environmental conditions, Schizosaccharomyces pombe can remain in the stationary phase or enter into either premitotic or premeiotic DNA synthesis. This decision point is known as Start. In the mitotic cell cycle, regulation of G1/S-specific gene expression is dependent upon the MBF (Mlu1 binding factor) complex, known to contain p85cdc10 and p72res1. Here we demonstrate that p73res2 controls cell cycle progression via its participation in the MBF complex, interacting directly with both p85cdc10 and p72res1. In contrast, when cells enter into meiosis, the MBF complex is disrupted, and p73res2 shifts its regulatory function towards the transactivation of genes required for meiotic progression. These observations suggest that p73res2 plays a pivotal role at Start and constitutes an example of a transcription factor involved in the control of both mitotic and meiotic progression.
authors.affiliation: Dana-Farber Cancer Institute and Harvard Medical School, Boston, Massachusetts 02115, USA.
authors.forename: J
authors.initials: J
authors.lastname: Ayté
authors.forename: J F
authors.initials: JF
authors.lastname: Leis
authors.forename: J A
authors.initials: JA
authors.lastname: DeCaprio
mesh_terms: D018797:Cell Cycle Proteins; D004268:DNA-Binding Proteins / Q000378:metabolism*; D019281:Dimerization; D005656:Fungal Proteins / Q000378:metabolism; D015966:Gene Expression Regulation, Fungal; D005800:Genes, Fungal; D008540:Meiosis / Q000502:physiology*; D008938:Mitosis / Q000502:physiology*; D008957:Models, Genetic; D009363:Neoplasm Proteins / Q000096:biosynthesis; D011233:Precipitin Tests; D011485:Protein Binding; D012568:Schizosaccharomyces / Q000502:physiology*; D029702:Schizosaccharomyces pombe Proteins; D014157:Transcription Factors
chemical_list: D018797:Cell Cycle Proteins; D004268:DNA-Binding Proteins; D005656:Fungal Proteins; D009363:Neoplasm Proteins; D029702:Schizosaccharomyces pombe Proteins; D014157:Transcription Factors; C078732:res1 protein, S pombe; C087021:res2 protein, S pombe