Comparison of BGB-MUG and LSTB-MUG in microbiological surveillance of recreational waters.
Recreational water surveillance is an important tool to prevent health hazards for the population. Therefore distinct guide and imperative values for fecal indicators are listed in the EC directive about water quality control. The detection methods, however, give laboratories some room to choose their own method, which has led to difficulties in the comparability of results. In 1989 an ad-hoc working group of the coastal countries of Germany established detection methods, which by now are obligatory for these countries. Fecal and total coliforms (FC and TC) are detected by a triplicate mpn-procedure using brilliant green-bile-lactose broth supplemented with tryptophane and 4-methylumbelliferyl-beta-D-glucuronide (BGB-MUG) as selective medium. Gas-, fluorescence- and indole-positive cultures are considered fecal coliform-positive. In the last years rises in TC but not in FC counts were observed in fresh waters. A study was carried out to evaluate the official method in another bathing season, to determine bacterial species leading to false-positive TC cultures and to compare BGB-MUG with laurylsulphate-tryptophane-MUG (LSTB-MUG). Water samples of different salinities and nutrient input were collected in weekly intervals from April to October. FC and TC concentrations were determined and all TC-positive cultures were differentiated further. The FC counts obtained by enrichment in BGB-MUG or LSTB-MUG were nearly identical, the rate of fluorescence-positive, indole-negative tubes being approximately 0.6%. Differentiation of FC-negative cultures showed a false-negative rate of 2.87% for BGB-MUG and of 8% for LSTB-MUG. During the summer months TC counts in BGB-MUG exceeded FC counts by far at most of the sampling sites. This effect was much less pronounced in LSTB-MUG; the difference between both enrichment media being significant. Differentiation of presumptive TC from BGB-MUG resulted in a high percentage of Aeromonas spp. in fresh waters. LSTB-MUG was clearly more selective for TC than BGB-MUG, but still with an average of 10% of the test tubes being false TC-positive (BGB-MUG 46%). The sensitivity of BGB-MUG was below 60% (LSTB-MUG 89%). LSTB-MUG should be preferred as enrichment medium in mpn-examination of recreational water, if no further differentiation is carried out. The selectivity for TC is better than in BGB-MUG and the only slight inhibitory effects can be tolerated.
Höller
C
C
Institut für Hygiene und Umweltmedizin, Christian-Albrechts-Universität Kiel.
Havemeister
G
G
Gundermann
K O
KO
D000333:Aeromonas / Q000302:isolation & purification; D001493:Bathing Beaches; D015169:Colony Count, Microbial; D003470:Culture Media; D004755:Enterobacteriaceae / Q000302:isolation & purification*; D005189:False Positive Reactions; D005243:Feces / Q000382:microbiology; D005618:Fresh Water / Q000382:microbiology; D005858:Germany; D006923:Hymecromone / Q000031:analogs & derivatives; D011786:Quality Control; D011998:Recreation; D012621:Seasons; D012967:Sodium Dodecyl Sulfate; D014364:Tryptophan; D014871:Water Microbiology
D003470:Culture Media; D012967:Sodium Dodecyl Sulfate; D006923:Hymecromone; C034888:4-methylumbelliferyl glucuronide; D014364:Tryptophan; C028913:dodecyl sulfate