Preparation and liquid chromatographic analysis of propanediol fatty acid esters.
Procedures were developed for the synthesis, purification and analysis of propanediol (PD) esters of n-fatty acids (FA). Mono- (MAPD) and di-acylated (DAPD) species were synthesized from PD and FA using an immobilized lipase (Candida antarctica B) in tert-butanol. MAPD and DAPD were isolated using silica gel column chromatography as approximately 95% pure preparations. Normal phase gradient LC provided for resolution of MAPD, DAPD and FA. UV220 nm detection provided a detection limit of about 1 microgram, and a linear response range of up to 2000 micrograms. Response factors were determined for MAPD, DAPD and FA components comprised of n-fatty acyl lengths of 4-16.
Chang
Q L
QL
Department of Food Science, University of Wisconsin, Madison 53706, USA.
Parkin
K L
KL
D002853:Chromatography, Liquid / Q000379:methods*; D004952:Esters; D005227:Fatty Acids / Q000737:chemistry* / Q000302:isolation & purification*; D011409:Propylene Glycols / Q000737:chemistry*; D015203:Reproducibility of Results; D013056:Spectrophotometry, Ultraviolet
D004952:Esters; D005227:Fatty Acids; D011409:Propylene Glycols