title: Time-resolved mid-infrared spectroscopy: methods and biological applications.
abstract: Recent developments in time-resolved infrared spectroscopy have paved the way to probe transient intermediates with a high degree of functional group specificity on timescales as short as femtoseconds. This capability has been exploited in studies of biophysical phenomena ranging from protein folding/unfolding to ligand migration in proteins.
authors.affiliation: Department of Chemistry & Chemical Biology, Harvard University, Cambridge, MA 02138, USA. slayton@fas.harvard.edu
authors.forename: R M
authors.initials: RM
authors.lastname: Slayton
authors.forename: P A
authors.initials: PA
authors.lastname: Anfinrud
mesh_terms: D001426:Bacterial Proteins / Q000737:chemistry; D003576:Electron Transport Complex IV / Q000737:chemistry; D010788:Photosynthesis; D011487:Protein Conformation; D017510:Protein Folding; D011506:Proteins / Q000737:chemistry* / Q000378:metabolism; D013055:Spectrophotometry, Infrared / Q000379:methods*; D013997:Time Factors
chemical_list: D001426:Bacterial Proteins; D011506:Proteins; D003576:Electron Transport Complex IV