title: Rac1 is required for cell proliferation and G2/M progression.
abstract: We have transiently expressed a dominant negative form of rac1 (N17rac1) using adenoviral-mediated gene transfer. The level of N17rac1 expression is demonstrated to be proportional to the multiplicity of infection. Expression of N17rac1 in Rat 2 fibroblasts results in cytostatic growth arrest. Cell-cycle analysis demonstrates that cells expressing N17rac1 accumulate in G2/M. These results suggest that rac1 is required for cell proliferation and provide the first demonstration in mammalian cells of a role for small GTP-binding proteins in the G2/M transition.
authors.affiliation: Cardiology Branch, National Heart, Lung and Blood Institute, Bethesda, MD 20892-1650, USA.
authors.forename: K A
authors.initials: KA
authors.lastname: Moore
authors.forename: R
authors.initials: R
authors.lastname: Sethi
authors.forename: A M
authors.initials: AM
authors.lastname: Doanes
authors.forename: T M
authors.initials: TM
authors.lastname: Johnson
authors.forename: J B
authors.initials: JB
authors.lastname: Pracyk
authors.forename: M
authors.initials: M
authors.lastname: Kirby
authors.forename: K
authors.initials: K
authors.lastname: Irani
authors.forename: P J
authors.initials: PJ
authors.lastname: Goldschmidt-Clermont
authors.forename: T
authors.initials: T
authors.lastname: Finkel
mesh_terms: D000256:Adenoviridae / Q000235:genetics; D000818:Animals; D015153:Blotting, Western; D002455:Cell Division / Q000187:drug effects / Q000235:genetics; D002460:Cell Line; D005347:Fibroblasts; D016195:G2 Phase / Q000187:drug effects / Q000235:genetics; D019204:GTP-Binding Proteins / Q000096:biosynthesis / Q000235:genetics / Q000502:physiology*; D018014:Gene Transfer Techniques; D008938:Mitosis / Q000187:drug effects / Q000235:genetics; D051381:Rats; D020744:rac GTP-Binding Proteins
chemical_list: D019204:GTP-Binding Proteins; D020744:rac GTP-Binding Proteins