Selective laser photocoagulation of blood vessels in a hamster skin flap model using a specific ICG formulation.
OBJECTIVE
The present study was undertaken to evaluate the selective laser photocoagulation of blood vessels in a hamster skin flap model using a specific indocyanine green (ICG) formulation.


METHODS
Experiments were performed in a hamster skin flap model after injection of ICG in aqueous solution (ICGA), or after injection of a specific formulation of ICG (ICG in emulsion: ICGE). Laser irradiation was achieved 30 minutes after injection with a 300 microns fiber connected to a 805 nm diode laser (power = 0.8W, spot diameter = 1.3 mm and pulse exposure time lasting from 1 to 5 s). Macroscopic observation and acute histology were performed to compare the tissue effects obtained for each ICG formulation and to assess the selectivity of vessel damage.


RESULTS
The ICGE clearance process was slowed down as compared to the ICGA process. After 30 minutes, the concentration of ICG in blood is higher (2.27 +/- 0.4, P < 0.003) for ICGE compared to ICGA. With ICGA, vessel coagulation required a minimum fluence of 240 J/cm2, which led to very significant skin damage. Conversely with ICGE, vessel coagulation required a fluence of 120 J/cm2. With such a fluence, no laser effect could be detected on the skin. Histological examination confirmed blood vessels coagulation in depth, whereas epidermis and dermis remained intact.


CONCLUSIONS
The major restrictions of ICG in aqueous solution, which are the very-short half-life of ICG in blood and consequently the lack of selectivity in blood vessels after a few minutes, are alleviated when ICG is used in emulsion. ICG in emulsion increases the circulating half-life of ICG and moreover confines ICG in the vascular compartment. Thanks to this specific property, it is possible to obtain a selective vascular damage 30 minutes after injection.
Mordon
S
S
Inserm (French National Institute of Health and Medical Research) Unit #279, Lille, France. serge.mordon@lille.inserm.fr
Desmettre
T
T
Devoisselle
J M
JM
Mitchell
V
V
D000818:Animals; D001808:Blood Vessels / Q000473:pathology; D006224:Cricetinae; D004655:Emulsions; D006207:Half-Life; D007208:Indocyanine Green / Q000008:administration & dosage / Q000378:metabolism*; D017075:Laser Coagulation / Q000379:methods*; D012867:Skin / Q000098:blood supply* / Q000473:pathology; D013050:Spectrometry, Fluorescence
D004655:Emulsions; D007208:Indocyanine Green