Interaction of spermine with dimyristoyl-L-alpha-phosphatidyl-DL-glycerol multilamellar liposomes.
Polycationic spermine interacts with the negative phosphate group of dimyristoylphosphatidylglycerol multilamellar liposomes, forming a positively charged shell around the vesicle surface. An association constant of (2.15+/-0.45) x 10(3) M(-1) between spermine and the phospholipid groups in liposomes has been evaluated by a new and rapid enzymatic method. ESR spectra show that the effects of this polycation on liposomes are substantially different from those of cations like Ca2+ and Mg2+ and confirm the ability of spermine to induce liposome aggregation and not fusion.
Stevanato
R
R
INFM, Department of Physical Chemistry, University of Venice, Italy.
Wisniewska
A
A
Momo
F
F
D002118:Calcium / Q000378:metabolism; D002412:Cations / Q000737:chemistry / Q000378:metabolism; D003497:Cyclic N-Oxides / Q000378:metabolism; D004578:Electron Spin Resonance Spectroscopy; D006863:Hydrogen-Ion Concentration; D008081:Liposomes / Q000737:chemistry / Q000378:metabolism*; D008274:Magnesium / Q000378:metabolism; D008968:Molecular Conformation; D010715:Phosphatidylglycerols / Q000378:metabolism*; D012964:Sodium / Q000378:metabolism; D013096:Spermine / Q000378:metabolism* / Q000494:pharmacology
D002412:Cations; D003497:Cyclic N-Oxides; D008081:Liposomes; D010715:Phosphatidylglycerols; D013096:Spermine; C032363:16-nitroxystearic acid; D012964:Sodium; C002773:dimyristoylphosphatidylglycerol; D008274:Magnesium; D002118:Calcium