Monitoring uptake of ellipticine and its fluorescence lifetime in relation to the cell cycle phase by flow cytometry.
Ellipticine (5,11-dimethyl-6H-pyrido[4,3-b]carbazole) is a green fluorescent plant alkaloid that inhibits DNA topoisomerase II activity and possesses pharmacologic activity toward both murine and human leukemias in vivo. In this flow cytometric study, the uptake of ellipticine was monitored as a function of cell volume and cell cycle phase in viable human promyelocytic (HL-60) cells costained with the DNA fluorochrome Hoechst 33342. Uptake of ellipticine was time and dose dependent; however, drug content was quantitatively similar in all phases of the cell cycle when normalized for DNA content or similar to cell size when correlated with cell volume. The fluorescence lifetime values of ellipticine in HL-60 cells, as analyzed by novel flow cytometric analysis, reached a plateau when the intra-cellular ellipticine intensity was still rising with increasing drug concentration. Since the free drug and the different subcellular ellipticine complexes, including DNA and RNA, had different lifetime values, the changes in the lifetime values appear to reflect differing proportions of unbound drug to that bound to different cellular constituents in the cells. Further development of phase-sensitive flow cytometry will provide for multiple lifetime determinations so that quantitation of drugs bound to the different cellular components can be performed along with the simultaneous determination of total drug uptake and cell cycle position. Such analyses should provide useful information for the design of drugs with greater affinity for cytotoxic targets.
Sailer
B L
BL
Life Sciences Division, Los Alamos National Laboratory, New Mexico 87545, USA.
Valdez
J G
JG
Steinkamp
J A
JA
Darzynkiewicz
Z
Z
Crissman
H A
HA
D000970:Antineoplastic Agents / Q000378:metabolism / Q000493:pharmacokinetics*; D002451:Cell Compartmentation; D002453:Cell Cycle / Q000502:physiology*; D048429:Cell Size; D002470:Cell Survival / Q000187:drug effects; D004273:DNA, Neoplasm / Q000032:analysis / Q000378:metabolism; D004305:Dose-Response Relationship, Drug; D004611:Ellipticines / Q000378:metabolism / Q000493:pharmacokinetics*; D005434:Flow Cytometry / Q000379:methods; D005456:Fluorescent Dyes / Q000493:pharmacokinetics; D018922:HL-60 Cells / Q000166:cytology* / Q000378:metabolism; D006801:Humans; D012334:RNA, Neoplasm / Q000032:analysis / Q000378:metabolism; D013997:Time Factors
D000970:Antineoplastic Agents; D004273:DNA, Neoplasm; D004611:Ellipticines; D005456:Fluorescent Dyes; D012334:RNA, Neoplasm; C034192:ellipticine