Nutritional requirements for growth of an endophyte: Ceratopycnidium baccharidicola.
The carbon sources, nitrogen sources and vitamin requirements for the growth of Ceratopycnidium baccharidicola (an endophyte of Baccharis coridifolia) were studied. The fungus utilized several carbon sources: pectin, sucrose, fructose, glucose, maltose, cellobiose, xylose, arabinose, mannitol, mannose and sorbitol. Sucrose and fructose were found to be the best carbon sources. Nitrogen sources utilized by the endophyte included: nitrates, ammonium and amino acids such as proline, asparagine and glycine. Undefined complex nitrogen sources such as soytone, tryptone, yeast extract and casamino acids supported excellent growth. In a defined medium, thiamine was the only vitamin required for growth. Under optimum conditions the vegetative growth of C. baccharidicola was enhanced six fold over its growth in glucose-asparagine medium.
Bertoni
M D
MD
Departamento de Ciencias Biológicas, Facultad de Ciencias Exactas y Naturales, Buenos Aires, Argentina.
Levin
L
L
D050260:Carbohydrate Metabolism; D002241:Carbohydrates / Q000494:pharmacology; D002244:Carbon / Q000378:metabolism; D003470:Culture Media / Q000494:pharmacology; D005658:Fungi / Q000187:drug effects / Q000254:growth & development / Q000378:metabolism*; D009172:Mycology / Q000379:methods; D009183:Mycotoxins / Q000096:biosynthesis; D009584:Nitrogen / Q000378:metabolism; D010944:Plants / Q000382:microbiology*; D013831:Thiamine / Q000378:metabolism / Q000494:pharmacology; D014815:Vitamins / Q000378:metabolism
D002241:Carbohydrates; D003470:Culture Media; D009183:Mycotoxins; D014815:Vitamins; D002244:Carbon; D009584:Nitrogen; D013831:Thiamine