Primary structure of Neurospora crassa gamma-tubulin.
We report the isolation and characterization of a cDNA-clone for gamma-tubulin from N. crassa. The deduced amino-acid sequence of the cDNA-clone most resembles that of gamma-tubulins from other fungi. Differential Southern blot analysis demonstrates that most probably a single gene represents gamma-tubulin. In addition, Northern blot analysis shows that most probably only one single mRNA encoding gamma-tubulin is transcribed in N. crassa. In a phylogenetic tree that includes all known gamma-tubulins, N. crassa gamma-tubulin is grouped, as expected, together with other fungal gamma-tubulins.
Heckmann
S
S
Adolf-Butenandt-Institute/Cell Biology, Munich, Germany.
Schliwa
M
M
Kube-Granderath
E
E
D000595:Amino Acid Sequence; D001483:Base Sequence; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics; D018628:Gene Dosage; D005800:Genes, Fungal / Q000235:genetics; D008969:Molecular Sequence Data; D009492:Neurospora crassa / Q000235:genetics*; D010802:Phylogeny; D012331:RNA, Fungal / Q000032:analysis; D012333:RNA, Messenger / Q000032:analysis; D017422:Sequence Analysis, DNA; D014404:Tubulin / Q000737:chemistry* / Q000235:genetics*
D018076:DNA, Complementary; D012331:RNA, Fungal; D012333:RNA, Messenger; D014404:Tubulin