title: Molecular approaches to identifying ligand binding and signaling domains of C-C chemokine receptors.
abstract: The construction of chimeric receptors provides a useful starting point for the identification of ligand-binding and G-protein-coupling sites on chemokine receptors. Correlation of the binding and signaling properties of a set of complementary receptor chimeras is a powerful approach for probing structure-function relationships. Further molecular resolution can subsequently be achieved by site-directed mutagenesis and/or alanine scanning.
authors.affiliation: Gladstone Institute of Cardiovascular Disease, University of California, San Francisco 94141, USA.
authors.forename: F S
authors.initials: FS
authors.lastname: Monteclaro
authors.forename: H
authors.initials: H
authors.lastname: Arai
authors.forename: I F
authors.initials: IF
authors.lastname: Charo
mesh_terms: D000595:Amino Acid Sequence; D001665:Binding Sites; D001667:Binding, Competitive; D002460:Cell Line; D002462:Cell Membrane / Q000378:metabolism; D019204:GTP-Binding Proteins / Q000378:metabolism; D006801:Humans; D007295:Inositol Phosphates / Q000378:metabolism; D008024:Ligands; D008958:Models, Molecular; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D011485:Protein Binding; D054389:Receptors, CCR1; D054390:Receptors, CCR2; D019707:Receptors, Chemokine / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*; D011993:Recombinant Fusion Proteins / Q000737:chemistry / Q000378:metabolism; D016415:Sequence Alignment; D015398:Signal Transduction / Q000502:physiology; D013379:Substrate Specificity; D014162:Transfection
chemical_list: C515300:CCR1 protein, human; C515334:CCR2 protein, human; D007295:Inositol Phosphates; D008024:Ligands; D054389:Receptors, CCR1; D054390:Receptors, CCR2; D019707:Receptors, Chemokine; D011993:Recombinant Fusion Proteins; D019204:GTP-Binding Proteins