Abnormalities in the development of the tectal projection from transplants of embryonic occipital cortex placed in the damaged occipital cortex of newborn rats.
We have examined the degree of precision in the topographic arrangement of the tectal projection developed by homotopic transplants of embryonic occipital cortex and tried to determine whether the development of the corticotectal projection is exclusively dependent on environmental cues or is also controlled by intrinsic factors. Transplants of embryonic (E16) occipital cortex were grafted into various areas of the occipital cortex (Oc1 or Oc2) of newborn rats and the organization of the tectal projection arising from the transplants was subsequently examined by injecting different neurotracers into the transplants. Our results indicate that in most cases the laminar and tangential distributions of the tectal projections from the transplants were abnormal. Indeed, whatever the location of the transplant in the host occipital cortex and whatever the placement of the injection into the transplant, a hybrid distribution of the tectal labeling was found, reminiscent of the pattern observed following tracer deposits in both Oc1 and Oc2 in intact animals. Since the grafts were composed of cells of both Oc1 and Oc2 embryonic origin, it is likely that the hybrid pattern of efferents reflects the heterogeneity of the embryonic origin of the cells composing the graft. These findings provide evidence that the development of the topographic distribution of neocortical efferents is not only dependent on factors extrinsic to the cortex and further indicate that even within one single cortical region, the occipital cortex, different areas (Oc1 vs Oc2) are not totally interchangeable. These findings might have important implications in transplantation experiments aiming at the reconstruction of damaged neocortical circuitry where a precise "point-to-point" reconstruction of the circuitry is expected.
Gaillard
A
A
UMR 6558, Département des Neurosciences, Université de Poitiers, France.
Létang
J
J
Frappé
I
I
Roger
M
M
D000818:Animals; D000831:Animals, Newborn; D001710:Biotin / Q000031:analogs & derivatives; D016380:Brain Tissue Transplantation / Q000473:pathology*; D002772:Cholera Toxin / Q000493:pharmacokinetics; D003911:Dextrans; D004525:Efferent Pathways / Q000473:pathology; D005260:Female; D016332:Fetal Tissue Transplantation / Q000473:pathology*; D005865:Gestational Age; D006735:Horseradish Peroxidase; D007150:Immunohistochemistry; D008297:Male; D009024:Morphogenesis; D009778:Occipital Lobe / Q000196:embryology / Q000293:injuries / Q000473:pathology / Q000637:transplantation*; D010835:Phytohemagglutinins; D051381:Rats; D017208:Rats, Wistar; D013477:Superior Colliculi / Q000473:pathology*; D014795:Visual Pathways / Q000473:pathology
D003911:Dextrans; D010835:Phytohemagglutinins; C076397:biotinylated dextran amine; C100156:leukoagglutinins, plants; D001710:Biotin; D002772:Cholera Toxin; D006735:Horseradish Peroxidase