Expression of chemokine RANTES and production of monoclonal antibodies.
RANTES was first identified as a cDNA in a search for genes expressed late (3-5 days) after T-cell activation. Definition of RANTES function depended on the generation of protein. This chapter describes the various techniques used to make recombinant RANTES protein, to test its activity, and to generate monoclonal antibodies to assess RANTES protein cell distribution.
Krensky
A M
AM
Department of Pediatrics, Stanford University Medical Center, California 94305-5119, USA.
Nelson
P J
PJ
D000911:Antibodies, Monoclonal / Q000096:biosynthesis / Q000276:immunology*; D015153:Blotting, Western; D002465:Cell Movement; D018946:Chemokine CCL5 / Q000096:biosynthesis / Q000235:genetics* / Q000276:immunology* / Q000494:pharmacology; D002630:Chemotactic Factors / Q000235:genetics / Q000302:isolation & purification / Q000494:pharmacology; D002634:Chemotaxis, Leukocyte; D004797:Enzyme-Linked Immunosorbent Assay; D004926:Escherichia coli / Q000235:genetics; D005786:Gene Expression Regulation; D005822:Genetic Vectors; D006801:Humans; D007150:Immunohistochemistry; D017403:In Situ Hybridization; D009240:N-Formylmethionine Leucyl-Phenylalanine / Q000494:pharmacology; D009838:Oligodeoxyribonucleotides / Q000235:genetics; D011233:Precipitin Tests; D011994:Recombinant Proteins / Q000276:immunology / Q000302:isolation & purification / Q000494:pharmacology
D000911:Antibodies, Monoclonal; D018946:Chemokine CCL5; D002630:Chemotactic Factors; D009838:Oligodeoxyribonucleotides; D011994:Recombinant Proteins; D009240:N-Formylmethionine Leucyl-Phenylalanine