Molecular cloning, cDNA sequence analysis, and chromosomal localization of mouse Pkd2.
The gene responsible for the second form of autosomal dominant polycystic kidney disease, PKD2, has recently been identified. We now describe the cloning, genomic localization, cDNA sequence, and expression analysis of its murine homologue, Pkd2. The cloned cDNA sequence is 5134 bp long and is predicted to encode a 966-amino-acid integral membrane protein with six membrane-spanning domains and intracellular NH2 and COOH termini. Pkd2 is highly conserved with 91% identity and 98% similarity to polycystin-2 at the amino acid level. Pkd2 mRNA is widely expressed in mouse tissues. Pkd2 maps to mouse Chromosome 5 and is excluded as a candidate gene for previously mapped mouse mutations resulting in a polycystic kidney phenotype.
Wu
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Department of Medicine, Albert Einstein College of Medicine, Bronx, New York 10461, USA.
Mochizuki
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T
Le
T C
TC
Cai
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Y
Hayashi
T
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Reynolds
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DM
Somlo
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S
D000595:Amino Acid Sequence; D000818:Animals; D002874:Chromosome Mapping; D003001:Cloning, Molecular; D018076:DNA, Complementary; D006801:Humans; D008565:Membrane Proteins / Q000235:genetics*; D051379:Mice; D008969:Molecular Sequence Data; D012333:RNA, Messenger / Q000235:genetics; D017386:Sequence Homology, Amino Acid; D050396:TRPP Cation Channels
D018076:DNA, Complementary; D008565:Membrane Proteins; D012333:RNA, Messenger; D050396:TRPP Cation Channels