title: Encapsidation of potyviral RNA in various forms of transgene coat protein is not correlated with resistance in transgenic plants.
abstract: Transgenic plants expressing either bean yellow mosaic potyvirus or chimeric potyvirus coat protein (CP) were inoculated with various potyviruses. Antigen-coated plate, indirect enzyme-linked immunosorbent assay and immunoelectron microscopy of virus purified from transgenic plants showed that progeny virions contained from < 1% to as much as 25% transgenic CP. Different levels of transcapsidation may reflect the extent of compatibility between transgene CP and the viral CP.
authors.affiliation: United States Department of Agriculture, Agricultural Research Service, United States National Arboretum, Beltsville, MD 20705, USA. jhammond@asrr.arsusda.gov
authors.forename: J
authors.initials: J
authors.lastname: Hammond
authors.forename: M M
authors.initials: MM
authors.lastname: Dienelt
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001042:Aphids; D002213:Capsid / Q000235:genetics* / Q000378:metabolism; D007303:Insect Vectors; D008969:Molecular Sequence Data; D010935:Plant Diseases / Q000821:virology*; D030821:Plants, Genetically Modified; D010947:Plants, Toxic; D017800:Potyvirus / Q000235:genetics / Q000254:growth & development* / Q000648:ultrastructure; D012367:RNA, Viral / Q000378:metabolism; D011994:Recombinant Proteins / Q000378:metabolism; D013045:Species Specificity; D014026:Nicotiana / Q000235:genetics / Q000821:virology
chemical_list: D012367:RNA, Viral; D011994:Recombinant Proteins