title: Synergism between the cellulosome-integrating protein CipA and endoglucanase CelD of Clostridium thermocellum.
abstract: The cellulosome-integrating protein CipA of Clostridium thermocellum was produced from a recombinant clone of Escherichia coli and purified by cellulose affinity and anion exchange chromatography. Two active forms of C. thermocellum endoglucanase CelD were tested for binding and hydrolytic activity on Avicel in the presence and in the absence of CipA. One was 68 kDa CelD, which contains an intact dockerin domain. The other was 65 kDa CelD, in which the dockerin domain is partially deleted. CipA promoted quantitative binding of 68 kDa CelD to Avicel and enhanced its Avicelase activity by at least ten-fold. By contrast, CipA had no effect on the activity nor on the cellulose-binding affinity of the truncated 65 kDa form. These results show that interaction between CipA and the catalytic component CelD is needed for the degradation of cellulose and confirm that the interaction is mediated by the dockerin domain of CelD.
authors.affiliation: Unité de Physiologie Cellulaire, Institut Pasteur, Paris, France.
authors.forename: V
authors.initials: V
authors.lastname: García-Campayo
authors.forename: P
authors.initials: P
authors.lastname: Béguin
mesh_terms: D001426:Bacterial Proteins / Q000302:isolation & purification / Q000494:pharmacology*; D002480:Cellulase / Q000378:metabolism*; D002482:Cellulose / Q000378:metabolism; D003013:Clostridium / Q000378:metabolism*; D008565:Membrane Proteins / Q000302:isolation & purification / Q000494:pharmacology*; D011994:Recombinant Proteins / Q000302:isolation & purification
chemical_list: D001426:Bacterial Proteins; C080369:CipA protein, Clostridium; D008565:Membrane Proteins; D011994:Recombinant Proteins; D002482:Cellulose; C087879:endoglucanase CelD; D002480:Cellulase