title: Effect of dietary aminoguanidine on tissue pentosidine and reproductive performance in broiler breeder hens.
abstract: Factors influencing the age-related decline in production parameters of broiler breeder hens are poorly understood. Elevated blood glucose concentrations measured in broiler breeder hens may contribute to this decline. The nonenzymatic attachment of glucose to proteins generates glycoxidation crosslinks in tissue proteins, which can ultimately impair their function. One such glycoxidation crosslink, pentosidine, has been used as a biomarker for aging studies because of its accumulation on the structural protein collagen. The objectives of these studies were to determine whether pentosidine accumulates with age in hens and whether the crosslinking inhibitor, aminoguanidine (AG), could retard this accumulation. An additional objective was to determine whether AG had any effect on production performance. In the first study, broiler breeder hens (n = 318) were randomly assigned to two groups: control and supplemented (400 ppm AG). Pentosidine was measured in the skin of the birds at 20 and 67 wk of age. Egg production was measured daily. In a second study, broiler breeder hens (n = 60) were reared as previously described. Pentosidine was measured in the skin of the birds at 20 and 68 wk of age. Results showed that pentosidine was present in the skin of the hens, and that concentrations increased with age (P < 0.001). Although pentosidine was reduced (P < 0.001) in AG-supplemented birds, production performance was not affected. In conclusion, AG retarded the rate of accumulation of pentosidine during lay in broiler breeder hens, but the reduction in pentosidine did not significantly affect production performance.
authors.affiliation: Division of Animal and Veterinary Sciences, West Virginia University, Morgantown 26506-6108, USA.
authors.forename: M
authors.initials: M
authors.lastname: Iqbal
authors.forename: L L
authors.initials: LL
authors.lastname: Probert
authors.forename: H
authors.initials: H
authors.lastname: Klandorf
mesh_terms: D000375:Aging / Q000378:metabolism / Q000502:physiology; D000704:Analysis of Variance; D000818:Animals; D001120:Arginine / Q000031:analogs & derivatives* / Q000032:analysis / Q000378:metabolism; D001786:Blood Glucose / Q000378:metabolism; D002645:Chickens / Q000378:metabolism / Q000502:physiology*; D003094:Collagen / Q000378:metabolism; D004032:Diet / Q000662:veterinary*; D004528:Egg Shell / Q000502:physiology; D005260:Female; D005298:Fertility / Q000502:physiology; D006146:Guanidines / Q000008:administration & dosage / Q000494:pharmacology*; D008239:Lysine / Q000031:analogs & derivatives* / Q000032:analysis / Q000378:metabolism; D008297:Male; D010058:Oviposition / Q000502:physiology; D011897:Random Allocation; D012098:Reproduction / Q000187:drug effects* / Q000502:physiology; D012867:Skin / Q000737:chemistry* / Q000378:metabolism; D013046:Specific Gravity
chemical_list: D001786:Blood Glucose; D006146:Guanidines; D003094:Collagen; D001120:Arginine; C062187:pentosidine; D008239:Lysine; C004479:pimagedine