Membrane-binding properties of phospholipase C-beta1 and phospholipaseC-beta2: role of the C-terminus and effects of polyphosphoinositides, G-proteins and Ca2+.
We have studied the binding of two G-protein-regulated phospholipase C (PLC) enzymes, PLCs-beta1 and -beta2, to membrane surfaces using sucrose-loaded bilayer phospholipid vesicles of varying compositions. Neither enzyme binds appreciably to pure phosphatidylcholine vesicles at lipid concentrations up to 10(-3) M. PLC-beta1 and PLC-beta2 bind vesicles composed of phosphatidylcholine, phosphatidylserine and phosphatidylethanolamine (molar ratio 1:1:1) with an approximate Kd of 10(-5) M. Inclusion of 2% PtdIns(4,5)P2 in these vesicles had no effect on the affinity of this interaction. As reported by others, removal of the C-terminus of PLC-beta1 and PLC-beta2 produces catalytically active fragments. The affinity of these truncated proteins for phospholipid vesicles is dramatically reduced suggesting that this region of the proteins contains residues important for membrane binding. Inclusion of G-protein alpha- and betagamma-subunit activators in the phospholipid vesicles does not increase the binding of PLC-beta1 or PLC-beta2, and the magnitude of G-protein-mediated PLC activation observed at low phospholipid concentrations (10(-6) M) is comparable to that observed at concentrations at which the enzymes are predominantly membrane-bound (10(-3) M). PLC-beta1 and -beta2 contain C2 domains but Ca2+ does not enhance binding to the vesicles. Our results indicate that binding of these enzymes to membranes involves the C-temini of the proteins and suggest that activation of these enzymes by G-proteins results from a regulated interaction between the membrane-bound proteins rather than G-protein-dependent recruitment of soluble enzymes to a substrate-containing phospholipid surface.
Jenco
J M
JM
Department of Pharmacological Sciences and Institute for Cell and Developmental Biology, Stony Brook Health Sciences Center, State University of New York at Stony Brook, Stony Brook, NY 11794-8651, USA.
Becker
K P
KP
Morris
A J
AJ
D000818:Animals; D002118:Calcium / Q000494:pharmacology*; D002417:Cattle; D019204:GTP-Binding Proteins / Q000378:metabolism*; D006801:Humans; D007527:Isoenzymes / Q000378:metabolism*; D007700:Kinetics; D008081:Liposomes; D046911:Macromolecular Substances; D018129:Phosphatidylinositol Phosphates / Q000494:pharmacology*; D054799:Phospholipase C beta; D010743:Phospholipids / Q000378:metabolism*; D011994:Recombinant Proteins / Q000378:metabolism; D013329:Structure-Activity Relationship; D013395:Sucrose; D010738:Type C Phospholipases / Q000378:metabolism*
D007527:Isoenzymes; D008081:Liposomes; D046911:Macromolecular Substances; D018129:Phosphatidylinositol Phosphates; D010743:Phospholipids; D011994:Recombinant Proteins; D013395:Sucrose; D010738:Type C Phospholipases; C518033:PLCB1 protein, human; C518036:PLCB2 protein, human; D054799:Phospholipase C beta; D019204:GTP-Binding Proteins; D002118:Calcium