Growth inhibition of Helicobacter pylori by a polyamine synthesis inhibitor, methylglyoxal bis(cyclopentylamidinohydrazone).
The anti-proliferative effect of methylglyoxal bis(cyclopentylamidino-hydrazone) (MGBCP), a multi-enzyme inhibitor of polyamine biosynthesis, on the growth of Helicobacter pylori was investigated. MGBCP inhibited the cell growth of H. pylori in a dose-dependent manner. The inhibition was partially reversed by the addition of spermidine. Synthesis of macromolecules, DNA, RNA and protein, was inhibited in the spermidine-depleted H. pylori cells. These findings suggest that MGBCP exhibits an anti-proliferative effect on H. pylori by suppression of macromolecule synthesis.
Takaji
S
S
Third Department of Internal Medicine, Mie University, Japan.
Hibasami
H
H
Imoto
I
I
Hashimoto
Y
Y
Nakao
K
K
Ikemura
N
N
Taguchi
Y
Y
Nakashima
K
K
D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D001426:Bacterial Proteins / Q000096:biosynthesis; D004269:DNA, Bacterial / Q000096:biosynthesis; D004791:Enzyme Inhibitors / Q000494:pharmacology; D016481:Helicobacter Infections / Q000188:drug therapy / Q000382:microbiology; D016480:Helicobacter pylori / Q000187:drug effects* / Q000254:growth & development / Q000378:metabolism*; D006801:Humans; D066298:In Vitro Techniques; D008935:Mitoguazone / Q000031:analogs & derivatives* / Q000494:pharmacology; D011073:Polyamines / Q000378:metabolism*; D011700:Putrescine / Q000096:biosynthesis; D012329:RNA, Bacterial / Q000096:biosynthesis; D013095:Spermidine / Q000096:biosynthesis
D000900:Anti-Bacterial Agents; D001426:Bacterial Proteins; D004269:DNA, Bacterial; D004791:Enzyme Inhibitors; D011073:Polyamines; D012329:RNA, Bacterial; C059234:methylglyoxal bis(cyclopentylamidinohydrazone); D008935:Mitoguazone; D013095:Spermidine; D011700:Putrescine