title: Inflammatory response after abdominal trauma, infection, or intestinal obstruction measured by oxygen radical production in peritoneal fluid.
abstract: BACKGROUND
  Reactive oxygen intermediates (ROI) have been implicated in many pathophysiological processes of inflammatory tissue damage and tissue repair. In the present study we compared the production of ROI in three different types of tissue damage in surgical patients.
  
  
  METHODS
  Peritoneal fluid specimens were harvested during the initial operation and postoperatively from 25 surgical patients with abdominal trauma, intraabdominal infection, and intestinal obstruction. The optical density at 412 nm, representing the peroxidation of hemoglobin, was measured to assess intraperitoneal ROI production. Patients were categorized into 3 groups: (A) infected patients with good outcome, (B) patients after trauma or obstruction with good outcome, and (C) patients with poor outcome due to persistent or secondary infection and multiple organ failure. Analysis of variance (ANOVA) and paired t test were used for statistical analysis.
  
  
  RESULTS
  Overall, the ROI production decreased significantly at days 2 and 3 compared with day 0 and 1 (P = 0.0013). No initial differences of intraoperative ROI concentrations were found among the three groups; however, patients with a poor outcome showed increased ROI values after 4 to 5 days (P = 0.038) when compared with the good outcome group.
  
  
  CONCLUSIONS
  We have demonstrated that intraperitoneal ROI production (1) can be measured in patients with intraabdominal tissue damage, (2) is not different between patients with intraabdominal infections, abdominal trauma, or intestinal obstruction, and (3) correlates with the clinical picture and the presence of an inflammatory intraabdominal focus or tissue damage.
authors.affiliation: Department of Surgery, University of Zürich, Switzerland.
authors.forename: M
authors.initials: M
authors.lastname: Heinzelmann
authors.forename: H P
authors.initials: HP
authors.lastname: Simmen
authors.forename: H
authors.initials: H
authors.lastname: Battaglia
authors.forename: H P
authors.initials: HP
authors.lastname: Friedl
authors.forename: O
authors.initials: O
authors.lastname: Trentz
mesh_terms: D018784:Abdominal Abscess / Q000378:metabolism* / Q000601:surgery; D000007:Abdominal Injuries / Q000378:metabolism* / Q000601:surgery; D001202:Ascitic Fluid / Q000737:chemistry; D016022:Case-Control Studies; D005260:Female; D006801:Humans; D007249:Inflammation / Q000378:metabolism; D007415:Intestinal Obstruction / Q000378:metabolism* / Q000601:surgery; D008297:Male; D008875:Middle Aged; D017382:Reactive Oxygen Species / Q000378:metabolism*
chemical_list: D017382:Reactive Oxygen Species