A conjugative linear plasmid in Streptomyces laurentii ATCC31255.
Plasmid pSLL of Streptomyces laurentii ATCC31255 (wild-type strain P0) is a 93-kilobase linear DNA plasmid that carries a protein bound to each 5' end of the DNA. It was self-transmitted to the pSLL-cured strain by conjugation in solid culture. The pSLL-cured strain carried a circular plasmid, pSLS, and showed a marked decrease in spore formation and thiostrepton productivity, owing to the pSLS. However, by retransmission of pSLL, these things reverted to levels seen in strain P0. Thus, plasmid pSLL suppressed the injurious effects of pSLS on the host mycelia.
Kinoshita-Iramina
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C
Microbial Genetics Division, Institute of Genetic Resources, Faculty of Agriculture, Kyushu University, Fukuoka, Japan.
Kitahara
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M
Doi
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K
Ogata
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S
D000900:Anti-Bacterial Agents / Q000096:biosynthesis; D015139:Blotting, Southern; D003227:Conjugation, Genetic / Q000235:genetics*; D003470:Culture Media; D004591:Electrophoresis, Polyacrylamide Gel; D005092:Exonucleases / Q000378:metabolism; D010957:Plasmids / Q000235:genetics*; D015183:Restriction Mapping; D013171:Spores, Bacterial / Q000235:genetics; D013302:Streptomyces / Q000201:enzymology / Q000235:genetics* / Q000254:growth & development; D013883:Thiostrepton / Q000096:biosynthesis
D000900:Anti-Bacterial Agents; D003470:Culture Media; D005092:Exonucleases; D013883:Thiostrepton