title: Computerized three-dimensional reconstruction reveals cerebrovascular regulatory subregions in rat brain stem.
abstract: Three-dimensional wireframe reconstructions were used to examine the relationship between the anatomical localization of electrode sites and the cerebrovascular response which was elicited by electrical stimulation of the dorsal raphe nucleus (DRN). Reconstructions of the rat brain and DRN were done from atlas plates and from Nissl-stained coronal sections (100-micron increments). Data points were entered and three-dimensional reconstructions were performed using commercially available software and a personal computer. Display of the entire brain yielded views which obscured visualization of the DRN. The data file was edited to reduce the number of contours without affecting the display resolution of the DRN. Selective display of the DRN and electronic rotation from the coronal to a sagittal view revealed a functional organization of the cerebral blood flow responses which was not apparent in two-dimensional coronal sections.
authors.affiliation: Laboratories of Neuropharmacology, Western Psychiatric Institute and Clinic, University of Pittsburgh, Pennsylvania 15213, USA.
authors.forename: M D
authors.initials: MD
authors.lastname: Underwood
authors.forename: V
authors.initials: V
authors.lastname: Arango
authors.forename: R W
authors.initials: RW
authors.lastname: Smith
authors.forename: M J
authors.initials: MJ
authors.lastname: Bakalian
authors.forename: J J
authors.initials: JJ
authors.lastname: Mann
mesh_terms: D000818:Animals; D001931:Brain Mapping / Q000295:instrumentation*; D001933:Brain Stem / Q000033:anatomy & histology / Q000502:physiology*; D002540:Cerebral Cortex / Q000098:blood supply; D002560:Cerebrovascular Circulation / Q000502:physiology*; D004558:Electric Stimulation; D007091:Image Processing, Computer-Assisted / Q000295:instrumentation*; D008297:Male; D008838:Microcomputers; D011903:Raphe Nuclei / Q000033:anatomy & histology / Q000502:physiology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D012039:Regional Blood Flow / Q000502:physiology; D012984:Software; D009435:Synaptic Transmission / Q000502:physiology*