title: Genotoxicity induced in human lymphoblasts by atmospheric reaction products of naphthalene and phenanthrene.
abstract: The genotoxic risks from exposure to polycyclic aromatic hydrocarbons (PAH) have long been recognized. Less well understood are the potential genotoxic risks of the atmospheric reaction products of this class of compounds. In this investigation, we have utilized several human cell genotoxicity assays to evaluate naphthalene, phenanthrene, and their atmospheric reaction products 1-nitronaphthalene, 2-nitronaphthalene, 1-hydroxy-2-nitronaphthalene, 2-hydroxy-1-nitronaphthalene, 1,4-naphthoquinone and 2-nitrodibenzopyranone. In addition, reaction products of naphthalene were generated in a 6700-1 Teflon environmental chamber, collected on a solid adsorbent, extracted and fractionated by normal-phase HPLC. Individual fractions were then analyzed using GC-MS, and tested for genotoxicity. Genotoxicity was determined using the human B-lymphoblastoid cell line, MCL-5, which expresses several transfected P450 and epoxide hydrolase genes. Mutagenicity was evaluated at both the heterozygous tk locus and the hemizygous hprt locus, permitting detection of both intragenic and chromosomal scale mutational events. Test compounds were also screened using the CREST modified micronucleus assay. Genotoxicity results indicate that 2-nitronaphthalene and 2-nitrodibenzopyranone possess greater mutagenic potency than their parent compounds, and interestingly, both compounds induced significant increases in mutation frequency at tk but not hprt. These results suggest a mechanistic difference in human cell response as compared to bacteria, where both compounds were previously shown to induce point mutations in the Salmonella reversion assay. The genotoxicity of 2-nitronaphthalene and 2-nitrodibenzopyranone in human cells, together with their high concentrations in ambient air relative to nitro-PAH directly emitted from combustion sources, emphasizes the need to consider atmospheric reaction products of PAH in genotoxicity assessments.
authors.affiliation: Environmental Toxicology Graduate Program, University of California, Riverside 92521, USA.
authors.forename: J C
authors.initials: JC
authors.lastname: Sasaki
authors.forename: J
authors.initials: J
authors.lastname: Arey
authors.forename: D A
authors.initials: DA
authors.lastname: Eastmond
authors.forename: K K
authors.initials: KK
authors.lastname: Parks
authors.forename: A J
authors.initials: AJ
authors.lastname: Grosovsky
mesh_terms: D000393:Air Pollutants / Q000737:chemistry / Q000633:toxicity*; D002460:Cell Line; D006801:Humans; D007041:Hypoxanthine Phosphoribosyltransferase / Q000235:genetics; D008214:Lymphocytes / Q000187:drug effects / Q000201:enzymology / Q000648:ultrastructure; D015162:Micronucleus Tests / Q000379:methods; D009152:Mutagenicity Tests / Q000379:methods; D009153:Mutagens / Q000737:chemistry / Q000633:toxicity*; D009154:Mutation; D009281:Naphthalenes / Q000737:chemistry / Q000633:toxicity*; D010616:Phenanthrenes / Q000737:chemistry / Q000633:toxicity*; D013937:Thymidine Kinase / Q000235:genetics
chemical_list: D000393:Air Pollutants; D009153:Mutagens; D009281:Naphthalenes; D010616:Phenanthrenes; D007041:Hypoxanthine Phosphoribosyltransferase; D013937:Thymidine Kinase