title: Glucokinase is located in secretory granules of pancreatic D-cells.
abstract: We immunohistochemically examined the distribution of glucokinase in rat pancreatic islets. Glucokinase immunoreactivity under light microscopy was detected in the cytoplasm of somatostatin cells as well as in that of insulin cells. No specific immunoreactivity was detected in glucagon and pancreatic polypeptide cells. In somatostatin cells, glucokinase immunoreactivity was located by electron microscopy exclusively within secretory granules.
authors.affiliation: Department of Clinical Biochemistry, Faculty of Pharmacy, Meijo University, Nagoya, Japan.
authors.forename: Y
authors.initials: Y
authors.lastname: Toyoda
authors.forename: S
authors.initials: S
authors.lastname: Yoshie
authors.forename: T
authors.initials: T
authors.lastname: Fujita
authors.forename: Y
authors.initials: Y
authors.lastname: Ito
authors.forename: T
authors.initials: T
authors.lastname: Nonogaki
authors.forename: I
authors.initials: I
authors.lastname: Miwa
mesh_terms: D000818:Animals; D003594:Cytoplasmic Granules / Q000201:enzymology*; D005934:Glucagon / Q000032:analysis; D005941:Glucokinase / Q000032:analysis*; D007150:Immunohistochemistry; D007328:Insulin / Q000032:analysis; D007515:Islets of Langerhans / Q000737:chemistry / Q000166:cytology / Q000201:enzymology; D008297:Male; D008854:Microscopy, Electron; D010191:Pancreatic Polypeptide / Q000032:analysis; D051381:Rats; D017208:Rats, Wistar; D013004:Somatostatin / Q000032:analysis; D019864:Somatostatin-Secreting Cells / Q000201:enzymology* / Q000648:ultrastructure
chemical_list: D007328:Insulin; D013004:Somatostatin; D010191:Pancreatic Polypeptide; D005934:Glucagon; D005941:Glucokinase