title: Backbone dynamics of oxidized and reduced D. vulgaris flavodoxin in solution.
abstract: Recombinant Desulfovibrio vulgaris flavodoxin was produced in Escherichia coli. A complete backbone NMR assignment for the two-electron reduced protein revealed significant changes of chemical shift values compared to the oxidized protein, in particular for the flavine mononucleotide (FMN)-binding site. A comparison of homo- and heteronuclear NOESY spectra for the two redox states led to the assumption that reduction is not accompanied by significant changes of the global fold of the protein. The backbone dynamics of both the oxidized and reduced forms of D. vulgaris flavodoxin were investigated using two-dimensional 15N-1H correlation NMR spectroscopy. T1, T2 and NOE data are obtained for 95% of the backbone amide groups in both redox states. These values were analysed in terms of the 'model-free' approach introduced by Lipari and Szabo [(1982) J. Am. Chem. Soc., 104, 4546-4559, 4559-4570]. A comparison of the two redox states indicates that in the reduced species significantly more flexibility occurs in the two loop regions enclosing FMN. Also, a higher amplitude of local motion could be found for the N(3)H group of FMN bound to the reduced protein compared to the oxidized state.
authors.affiliation: Institute of Biophysical Chemistry, Frankfurt, Germany.
authors.forename: A
authors.initials: A
authors.lastname: Hrovat
authors.forename: M
authors.initials: M
authors.lastname: Blümel
authors.forename: F
authors.initials: F
authors.lastname: Löhr
authors.forename: S G
authors.initials: SG
authors.lastname: Mayhew
authors.forename: H
authors.initials: H
authors.lastname: Rüterjans
mesh_terms: D001665:Binding Sites; D003198:Computer Simulation; D018360:Crystallography, X-Ray / Q000379:methods; D016969:Desulfovibrio vulgaris; D004926:Escherichia coli; D005486:Flavin Mononucleotide / Q000378:metabolism; D005418:Flavodoxin / Q000737:chemistry* / Q000378:metabolism*; D008961:Models, Structural; D019906:Nuclear Magnetic Resonance, Biomolecular / Q000379:methods; D010084:Oxidation-Reduction; D011487:Protein Conformation; D011994:Recombinant Proteins / Q000737:chemistry / Q000378:metabolism; D012996:Solutions
chemical_list: D005418:Flavodoxin; D011994:Recombinant Proteins; D012996:Solutions; D005486:Flavin Mononucleotide