title: Role of intracellular redox status in apoptosis induction of human T-cell leukemia virus type I-infected lymphocytes by 13-cis-retinoic acid.
abstract: We have shown that cell cycle progression of human T-cell leukemia virus type I (HTLV-I)-transformed T-cell lines was inhibited by 13-cis-retinoic acid (13cRA). In the present study, we report that 13cRA inhibited proliferation and induced cell death of peripheral blood mononuclear cells obtained from four patients with acute adult T-cell leukemia but not of mitogen- or interleukin 2-activated peripheral blood mononuclear cells from HTLV-I-negative healthy donors. Because HTLV-I-infected lymphocytes are susceptible to oxidative stress, we examined the role of the intracellular redox state in 13cRA-induced cell death using a HTLV-I-positive T-cell line, ATL2, as a model. 13cRA induced apoptosis in ATL2 cells within 48 h in a dose-dependent manner. The ability of 13cRA to induce apoptosis was more potent than that of all-trans-retinoic acid. Apoptosis induction by 13cRA was significantly enhanced by buthionine sulfoximine (BSO), which decreased the levels of intracellular reduced glutathione, although 13cRA by itself did not alter them, suggesting that intracellular reduced glutathione may modulate 13cRA-induced apoptosis. In addition, flow cytometric analysis revealed that 13cRA increased intracellular peroxides in 24 h and that the addition of BSO further enhanced them. Although N-acetylcysteine had only a marginal effect, pretreatment with catalase markedly inhibited 13cRA-induced apoptosis. These results suggest that peroxide generation, ie., oxidative stress, may play a crucial role in the induction of apoptosis by 13cRA and further demonstrate that combined treatment with 13cRA and BSO induces apoptosis of HTLV-I-positive lymphocytes even more potently.
authors.affiliation: Department of Biological Responses, Institute for Virus Research, College of Medical Technology, Kyoto University, Japan.
authors.forename: K
authors.initials: K
authors.lastname: Furuke
authors.forename: T
authors.initials: T
authors.lastname: Sasada
authors.forename: Y
authors.initials: Y
authors.lastname: Ueda-Taniguchi
authors.forename: A
authors.initials: A
authors.lastname: Yamauchi
authors.forename: T
authors.initials: T
authors.lastname: Inamoto
authors.forename: Y
authors.initials: Y
authors.lastname: Yamaoka
authors.forename: H
authors.initials: H
authors.lastname: Masutani
authors.forename: J
authors.initials: J
authors.lastname: Yodoi
mesh_terms: D000328:Adult; D017209:Apoptosis / Q000187:drug effects*; D019328:Buthionine Sulfoximine / Q000494:pharmacology; D002374:Catalase / Q000494:pharmacology; D002455:Cell Division / Q000187:drug effects; D005978:Glutathione / Q000187:drug effects / Q000378:metabolism; D006801:Humans; D006861:Hydrogen Peroxide / Q000378:metabolism; D015474:Isotretinoin / Q000494:pharmacology*; D007641:Keratolytic Agents / Q000494:pharmacology*; D015459:Leukemia-Lymphoma, Adult T-Cell / Q000378:metabolism / Q000503:physiopathology*; D007963:Leukocytes, Mononuclear / Q000166:cytology / Q000187:drug effects; D010084:Oxidation-Reduction; D018384:Oxidative Stress / Q000502:physiology*; D011518:Proto-Oncogene Proteins / Q000187:drug effects / Q000378:metabolism; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000187:drug effects / Q000378:metabolism; D051028:bcl-2-Associated X Protein
chemical_list: D007641:Keratolytic Agents; D011518:Proto-Oncogene Proteins; D019253:Proto-Oncogene Proteins c-bcl-2; D051028:bcl-2-Associated X Protein; D019328:Buthionine Sulfoximine; D006861:Hydrogen Peroxide; D002374:Catalase; D015474:Isotretinoin; D005978:Glutathione