title: Construction of an approximately 2 Mb contig in the region around 80 cM of Arabidopsis thaliana chromosome 2.
abstract: A method for construction of bacterial artificial chromosome (BAC) contigs from a yeast artificial chromosome (YAC) physical map is described. An approximately 2 Mb contig, consisting of two large BAC contigs linked by a small YAC, has been assembled in the region around 80 cM of Arabidopsis thaliana chromosome 2. Clones from this contig will facilitate gene isolation in the region and can be used directly as substrates for DNA sequencing.
authors.affiliation: Department of Genetics, Harvard Medical School, Boston, MA, USA.
authors.forename: M L
authors.initials: ML
authors.lastname: Wang
authors.forename: L
authors.initials: L
authors.lastname: Huang
authors.forename: D K
authors.initials: DK
authors.lastname: Bongard-Pierce
authors.forename: S
authors.initials: S
authors.lastname: Belmonte
authors.forename: E A
authors.initials: EA
authors.lastname: Zachgo
authors.forename: J W
authors.initials: JW
authors.lastname: Morris
authors.forename: M
authors.initials: M
authors.lastname: Dolan
authors.forename: H M
authors.initials: HM
authors.lastname: Goodman
mesh_terms: D017360:Arabidopsis / Q000235:genetics*; D002736:Chloroplasts / Q000235:genetics; D002874:Chromosome Mapping; D002875:Chromosomes; D018244:Chromosomes, Artificial, Yeast; D003001:Cloning, Molecular; D018744:DNA, Plant / Q000737:chemistry*; D004275:DNA, Ribosomal / Q000737:chemistry; D017422:Sequence Analysis, DNA
chemical_list: D018744:DNA, Plant; D004275:DNA, Ribosomal