Autoantibody reactive with the human general transcription factor TFIIF in sera from patients with autoimmune disorders.
Transcription factor (TF) IIF, a heteromeric protein composed of two subunits, RAP30 and RAP74, is required for both specific initiation and elongation of mRNA synthesis by RNA polymerase II. We have identified high titre of specific autoantibodies against the RAP74 subunit of TFIIF in sera from patients with systemic lupus erythematosus (SLE) as well as those with rheumatoid arthritis (RA), periarteritis nodosa (PN), Sjögren's syndrome (SS), dermatomyositis (DM), and mixed connective tissue disease (MCTD) by Western blot or immunoprecipitation. The epitopes recognized by autoantibodies were shown to be preferentially clustered at the central charged region. Anti-RAP74 autoantibody was shown to suppress the activity of TFIIF-stimulated elongation of mRNA synthesis by RNA polymerase II. It is concluded that some patients with autoimmune disorders develop specific autoantibodies against the RAP74 subunit of TFIIF.
Cai
Y
Y
Department of Clinical Chemistry and Laboratory Medicine, Faculty of Medicine, Kyushu University, Fukuoka, Japan.
Kitajima
S
S
Etoh
F
F
Kinoshita
S
S
Okubo
K
K
Hamasaki
N
N
D000328:Adult; D000368:Aged; D019138:Antibodies, Blocking; D001172:Arthritis, Rheumatoid / Q000097:blood / Q000276:immunology; D001323:Autoantibodies / Q000032:analysis* / Q000276:immunology*; D001327:Autoimmune Diseases / Q000097:blood / Q000276:immunology*; D015153:Blotting, Western; D002478:Cells, Cultured; D012321:DNA-Directed RNA Polymerases / Q000378:metabolism; D003882:Dermatomyositis / Q000097:blood / Q000276:immunology; D000939:Epitopes / Q000276:immunology; D005260:Female; D006367:HeLa Cells; D006801:Humans; D008180:Lupus Erythematosus, Systemic / Q000097:blood / Q000276:immunology; D008297:Male; D008875:Middle Aged; D008947:Mixed Connective Tissue Disease / Q000097:blood / Q000276:immunology; D010488:Polyarteritis Nodosa / Q000097:blood / Q000276:immunology; D011233:Precipitin Tests; D012313:RNA / Q000378:metabolism; D012859:Sjogren's Syndrome / Q000097:blood / Q000276:immunology; D014157:Transcription Factors / Q000276:immunology* / Q000378:metabolism; D019473:Transcription Factors, TFII
D019138:Antibodies, Blocking; D001323:Autoantibodies; D000939:Epitopes; D014157:Transcription Factors; D019473:Transcription Factors, TFII; D012313:RNA; D012321:DNA-Directed RNA Polymerases; C056604:transcription factor TFIIF