Transient expression of goat growth hormone gene in poplar (Populus alba L.) protoplasts: a quick method for detection of foreign gene expression in mRNA level.
We developed a sensitive, accurate, and fast method to detect foreign gene expression using reverse transcriptase-mediated polymerase chain reaction (RT-PCR) in poplar tree (Populus alba L.) protoplasts. Template total RNA was purified by removing the transfected foreign gene vector completely with DNase I treatment before the RT reaction. Expression of cDNA that encodes goat growth hormone was confirmed at the mRNA level 24 h after electroporation mediated DNA transfer.
Qiao
J
J
Wood Research Institute, Kyoto University, Japan.
Ishihara
Y
Y
Kuroda
H
H
Sakai
F
F
Sakai
H
H
Komano
T
T
D000818:Animals; D004247:DNA / Q000378:metabolism; D003850:Deoxyribonuclease I / Q000378:metabolism; D018506:Gene Expression Regulation, Plant; D006041:Goats / Q000378:metabolism*; D013006:Growth Hormone / Q000096:biosynthesis* / Q000235:genetics*; D010944:Plants / Q000378:metabolism*; D016133:Polymerase Chain Reaction; D011523:Protoplasts / Q000378:metabolism*; D012313:RNA / Q000096:biosynthesis; D012333:RNA, Messenger / Q000096:biosynthesis* / Q000235:genetics
D012333:RNA, Messenger; D012313:RNA; D013006:Growth Hormone; D004247:DNA; D003850:Deoxyribonuclease I