title: An isolated perfused human placental lobule model for multiple indicator dilution studies.
abstract: We describe a method for multiple indicator dilution studies in the isolated perfused human placental lobule developed to investigate the relationships between changes in pressure and flow and solute clearance. A peripheral lobule of a human placenta is perfused with a tissue culture-based medium and the perfusate oxygen tension, arterial and venous pressures, pH and perfusion temperature continuously monitored by a computerized system. Flow rates are readily changed. Bolus injections of vascular, extracellular and water space markers, and study compounds can be made into either maternal or fetal circulations, and precisely timed outflow fractions can be collected with computer-controlled fraction collectors, allowing simultaneous determination of concentration-time profiles of each marker.
authors.affiliation: Conjoint Endocrine Laboratory, Royal Brisbane Hospital, Queensland, Australia.
authors.forename: R L
authors.initials: RL
authors.lastname: Rasiah
authors.forename: R S
authors.initials: RS
authors.lastname: Addison
authors.forename: M S
authors.initials: MS
authors.lastname: Roberts
authors.forename: R H
authors.initials: RH
authors.lastname: Mortimer
mesh_terms: D005260:Female; D006801:Humans; D008431:Maternal-Fetal Exchange / Q000502:physiology*; D008954:Models, Biological; D010477:Perfusion / Q000295:instrumentation*; D010920:Placenta / Q000098:blood supply / Q000502:physiology*; D011247:Pregnancy; D011865:Radioisotope Dilution Technique; D012039:Regional Blood Flow / Q000502:physiology