Microdialysis-immunoaffinity capillary electrophoresis studies on neuropeptide-induced lymphocyte secretion.
A micro-sampling procedure has been developed for studying lymphocyte secretion of biologically important peptides in low cell density cultures. The technique is based on microdialysis recovery of the analytes of interest coupled with immunoaffinity capillary electrophoresis separation of the microdialysis samples and laser-induced fluorescence detection. Although the technique is able to recover secreted materials only at the 5-10 cell level, the detection system has a limit of detection (LOD) in the attomole (10(-18) M) range. This degree of sensitivity indicates that the system has the potential to measure secreted products at the single cell level. An added advantage of this system over other sampling techniques is that the microdialysis probe allows continuous sampling over time.
Phillips
T M
TM
Immunochemistry Laboratory, George Washington University Medical Center, Washington, DC 20037, USA.
Kennedy
L M
LM
De Fabo
E C
EC
D000918:Antibody Specificity; D016207:Cytokines / Q000378:metabolism*; D019075:Electrophoresis, Capillary / Q000379:methods; D006801:Humans; D007834:Lasers; D017551:Microdialysis; D012680:Sensitivity and Specificity; D013050:Spectrometry, Fluorescence; D013373:Substance P / Q000378:metabolism*; D013601:T-Lymphocytes / Q000276:immunology / Q000378:metabolism*
D016207:Cytokines; D013373:Substance P