Experimental assessment of the detection limit of genomic amplification by comparative genomic hybridization CGH.
Artificial amplicons of known size, constructed by use of YACs featuring human 8p12 and 12q13, were analyzed by comparative genomic hybridization (CGH). A minimum of 15 Mb of overrepresented DNA sequences could be detected. The sensitivity is (1) not dependent on the chromosome site and (2) related to the size of the amplicon, decreasing with decreasing size.
Parente
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Laboratoire de Génétique Chromosomique des Tumeurs, UMR 6549, CNRS/Université de Nice-Sophia Antipolis, France.
Gaudray
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Carle
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Turc-Carel
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C
D002874:Chromosome Mapping / Q000379:methods*; D018244:Chromosomes, Artificial, Yeast; D004271:DNA, Fungal / Q000032:analysis; D005819:Genetic Markers; D015894:Genome, Human; D006801:Humans; D009693:Nucleic Acid Hybridization / Q000379:methods*; D012680:Sensitivity and Specificity
D004271:DNA, Fungal; D005819:Genetic Markers