title: Protective effect of hexetidine against in vitro bacterial demineralisation of bovine enamel and dentin in the presence of fluoride.
abstract: Bovine enamel and dentin specimens were overlaid with acidogenic Streptococcus mutans suspensions in agarose. In this model, the minimal demineralisation-inhibiting concentrations (MDIC) of hexetidine was determined in the presence of fluoride. A commercially available mouthwash containing 0.1% (2.9 mmol/l) hexetidine was diluted serially and added to the bacterial suspensions together with 0, 5.3, or 26.3 mumol/l fluoride (NaF). After 22 h of incubation at 37 degrees C the bacterial suspensions were removed and assessed for calcium and lactate. The results showed significant inhibitory effects of hexetidine on the demineralisation of the enamel specimens with a MDIC between 15 and 31 mumol/l hexetidine. In the presence of fluoride, approximately fourfold higher concentrations of hexetidine were needed for a significant additional protection of the enamel. No synergistic effect between hexetidine and fluoride was observed. For the demineralisation of the dentin specimens, the MDIC of hexetidine had a value between 31 and 61 mumol/l. At both these concentrations the dentin specimens were relatively less protected in the presence than in the absence of fluoride, and some synergistic effect between hexeditine and fluoride was observed.
authors.affiliation: Department of Cariology and Endodontology, Academic Centre for Dentistry Amsterdam (ACTA), The Netherlands.
authors.forename: R
authors.initials: R
authors.lastname: Chmelarová
authors.forename: C
authors.initials: C
authors.lastname: Van Loveren
authors.forename: J F
authors.initials: JF
authors.lastname: Buijs
authors.forename: J M
authors.initials: JM
authors.lastname: ten Cate
mesh_terms: D000818:Animals; D000891:Anti-Infective Agents, Local / Q000627:therapeutic use*; D002118:Calcium / Q000032:analysis; D002327:Cariostatic Agents / Q000627:therapeutic use*; D002417:Cattle; D003470:Culture Media; D003743:Dental Enamel / Q000187:drug effects* / Q000382:microbiology; D003804:Dentin / Q000187:drug effects* / Q000382:microbiology; D004195:Disease Models, Animal; D004347:Drug Interactions; D004357:Drug Synergism; D005459:Fluorides / Q000627:therapeutic use*; D005460:Fluorides, Topical / Q000627:therapeutic use; D006590:Hexetidine / Q000627:therapeutic use*; D007773:Lactates / Q000032:analysis; D009067:Mouthwashes / Q000627:therapeutic use; D012685:Sepharose; D012969:Sodium Fluoride / Q000627:therapeutic use; D013295:Streptococcus mutans / Q000187:drug effects*; D013997:Time Factors; D017001:Tooth Demineralization / Q000382:microbiology / Q000517:prevention & control*
chemical_list: D000891:Anti-Infective Agents, Local; D002327:Cariostatic Agents; D003470:Culture Media; D005460:Fluorides, Topical; D007773:Lactates; D009067:Mouthwashes; D006590:Hexetidine; D012969:Sodium Fluoride; D012685:Sepharose; D005459:Fluorides; D002118:Calcium