title: Identification and subcellular localization of a novel mammalian dynamin-related protein homologous to yeast Vps1p and Dnm1p.
abstract: The dynamin family of GTP-binding proteins are implicated in vesicular transport. These include mammalian dynamins I, II, III, and yeast Vps1p and Dnm1p. Dynamin is involved in the formation of clathrin-coated vesicles at the plasma membrane. On the other hand, Vps1p and Dnm1p appear to be involved in transport from the late Golgi compartment to vacuoles and in an endocytic process, respectively. In this study, we identified a novel human protein, named Dnm1p/Vps1p-like protein (DVLP). It resembled more closely Dnm1p and Vps1p than dynamins not only in the primary structure but also in the domain organization. DVLP mRNA was expressed ubiquitously, suggesting that this protein plays a fundamental role in cellular function. Immunofluorescence analysis of cells expressing epitope-tagged DVLP revealed that it showed a diffused perinuclear staining pattern that was not superimposed on that of the marker protein for the Golgi apparatus, trans-Golgi network, lysosomes, endosomes, or endoplasmic reticulum. These data suggest that DVLP is not involved in the formation of known coated vesicles.
authors.affiliation: Institute of Biological Sciences, University of Tsukuba, Tsukuba Science City, Ibaraki.
authors.forename: H W
authors.initials: HW
authors.lastname: Shin
authors.forename: C
authors.initials: C
authors.lastname: Shinotsuka
authors.forename: S
authors.initials: S
authors.lastname: Torii
authors.forename: K
authors.initials: K
authors.lastname: Murakami
authors.forename: K
authors.initials: K
authors.lastname: Nakayama
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002352:Carrier Proteins / Q000032:analysis* / Q000235:genetics / Q000378:metabolism; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics; D034284:Dynamin I; D034286:Dynamin III; D034281:Dynamins; D005656:Fungal Proteins / Q000032:analysis* / Q000235:genetics / Q000378:metabolism; D020558:GTP Phosphohydrolases / Q000032:analysis* / Q000235:genetics / Q000378:metabolism; D019204:GTP-Binding Proteins; D006801:Humans; D051379:Mice; D008869:Microtubule-Associated Proteins; D024101:Mitochondrial Proteins; D008969:Molecular Sequence Data; D011506:Proteins / Q000032:analysis* / Q000235:genetics; D051381:Rats; D012441:Saccharomyces cerevisiae / Q000235:genetics / Q000378:metabolism; D029701:Saccharomyces cerevisiae Proteins; D017386:Sequence Homology, Amino Acid; D013347:Subcellular Fractions / Q000737:chemistry / Q000378:metabolism; D033921:Vesicular Transport Proteins
chemical_list: D002352:Carrier Proteins; D018076:DNA, Complementary; D005656:Fungal Proteins; D008869:Microtubule-Associated Proteins; D024101:Mitochondrial Proteins; D011506:Proteins; D029701:Saccharomyces cerevisiae Proteins; D033921:Vesicular Transport Proteins; D034284:Dynamin I; D020558:GTP Phosphohydrolases; D019204:GTP-Binding Proteins; C064553:VPS1 protein, S cerevisiae; C094774:DNM1 protein, S cerevisiae; C110768:DNM1L protein, human; C486391:Dnm1l protein, mouse; C519851:Dnm1l protein, rat; D034286:Dynamin III; D034281:Dynamins