title: Hydroxyapatite adherence as a means to concentrate bacteria.
abstract: Adherence to hydroxyapatite (HA) was examined as a method to concentrate bacteria from foods. Using HA at a level of 10% and suspensions of an Escherichia coli strain containing 10(9), 10(6), and 10(3) cells per ml, kinetic studies revealed that maximum adherence was attained within 5 min for all cell concentrations and that comparable log reductions (1.0 to 1.5) of cells in suspension were seen regardless of initial cell concentration. Eleven species of spoilage and pathogenic bacteria were found to adhere to HA, with seven species adhering at proportions of greater than 95%. Fluorescent viability staining revealed that cells bound to HA remained viable. There was greater than 92% adherence of indigenous bacteria to HA from three of five 1:10 dilutions of ground beef, indicating promise for the use of HA for concentrating bacteria from meat and other food samples.
authors.affiliation: Roman L. Hruska U.S. Meat Animal Research Center, U.S. Department of Agriculture, Clay Center, Nebraska 68933-0166, USA.
authors.forename: E D
authors.initials: ED
authors.lastname: Berry
authors.forename: G R
authors.initials: GR
authors.lastname: Siragusa
mesh_terms: D000818:Animals; D001422:Bacterial Adhesion; D001431:Bacteriological Techniques; D002417:Cattle; D017886:Durapatite; D004926:Escherichia coli / Q000302:isolation & purification; D019453:Escherichia coli O157 / Q000302:isolation & purification; D005243:Feces / Q000382:microbiology; D005456:Fluorescent Dyes; D005516:Food Microbiology; D007700:Kinetics; D008460:Meat / Q000382:microbiology
chemical_list: D005456:Fluorescent Dyes; D017886:Durapatite