title: Effect of linoleic acid, linoleic acid anilide, and arachidonic acid on the expression of adhesion molecules on human neutrophils.
abstract: The effects of linoleic acid, linoleic acid anilide, and arachidonic acid on the expression of CD11b/ CD18, CD11c/CD18 integrins and L-selectin on human neutrophils were studied by flow cytometry in a whole blood assay. None of these compounds had any effect on the basal expression of CD11b, CD11c, or L-selectin in the concentration range of 20-100 microM. However, linoleic acid at a concentration of 1000 microM slightly up-regulated CD11b and CD11c by a factor of 2.1 and 1.7, respectively. Linoleic acid, linoleic acid anilide, and arachidonic acid did not affect the formyl-methionyl-leucyl-phenylalanine induced up-regulation of CD11b or CD11c. However, linoleic acid and linoleic acid anilide slightly inhibited the phorbol myristate acetate (PMA)-induced expression of CD11b, which was decreased by 27 and 21% at concentrations of 100 and 1000 microM, respectively. Likewise, arachidonic acid at 40 microM inhibited the PMA-induced expression of CD11b by 19%. Our results suggest that linoleic acid, linoleic acid anilide, and arachidonic acid do not dramatically affect the expression of leukocyte adhesion molecules in a whole blood assay.
authors.affiliation: University of Kuopio, Department of Pharmacology and Toxicology, Finland.
authors.forename: K M
authors.initials: KM
authors.lastname: Heiskanen
authors.forename: S
authors.initials: S
authors.lastname: Münzing
authors.forename: F
authors.initials: F
authors.lastname: Krombach
authors.forename: K M
authors.initials: KM
authors.lastname: Savolainen
mesh_terms: D000813:Anilides / Q000633:toxicity*; D016718:Arachidonic Acid / Q000633:toxicity*; D018821:CD18 Antigens / Q000096:biosynthesis / Q000235:genetics; D015815:Cell Adhesion Molecules / Q000096:biosynthesis* / Q000235:genetics; D005434:Flow Cytometry; D006801:Humans; D016167:Integrin alphaXbeta2 / Q000096:biosynthesis / Q000235:genetics; D019041:L-Selectin / Q000096:biosynthesis / Q000235:genetics; D019787:Linoleic Acid / Q000633:toxicity*; D008041:Linoleic Acids / Q000633:toxicity*; D016177:Macrophage-1 Antigen / Q000096:biosynthesis / Q000235:genetics; D009240:N-Formylmethionine Leucyl-Phenylalanine; D009504:Neutrophils / Q000187:drug effects* / Q000378:metabolism; D016028:Receptors, Leukocyte-Adhesion / Q000096:biosynthesis* / Q000235:genetics; D013755:Tetradecanoylphorbol Acetate
chemical_list: D000813:Anilides; D018821:CD18 Antigens; D015815:Cell Adhesion Molecules; D016167:Integrin alphaXbeta2; D008041:Linoleic Acids; D016177:Macrophage-1 Antigen; D016028:Receptors, Leukocyte-Adhesion; D019041:L-Selectin; C039294:linoleylanilide; D016718:Arachidonic Acid; D009240:N-Formylmethionine Leucyl-Phenylalanine; D019787:Linoleic Acid; D013755:Tetradecanoylphorbol Acetate