title: Chronic renal blood flow measurement in dogs by transit-time ultrasound flowmetry.
abstract: To test the validity of transit-time ultrasound flowmetry for chronic measurement of renal blood flow in dogs, we compared this method with the renal clearance of para-aminohippuric acid (CPAH) (corrected for hematocrit), and with direct volumetric measurements. When flow-probes were implanted without silastic sheeting to stabilize the implant, there was significant disparity between the (within-dog) mean levels of renal blood flow estimated by flow-probe and CPAH. In contrast, when the flow-probe implants were stabilized with silicone sheeting, there was close agreement in each dog between the flow rates measured by the two methods. When flow-probes were calibrated volumetrically in situ, there was a close linear relationship between flow derived from the flow-probe and that measured volumetrically (r = 0.98 +/- 0.02). We conclude that valid, chronic measurement of renal blood flow in dogs can be achieved using transit-time ultrasound flowmetry, provided the implant is stabilized with silicone sheeting.
authors.affiliation: Baker Medical Research Institute, Prahran, Victoria, Australia. roger.evans@med.monash.edu.au
authors.forename: R G
authors.initials: RG
authors.lastname: Evans
authors.forename: K M
authors.initials: KM
authors.lastname: Stevenson
authors.forename: S C
authors.initials: SC
authors.lastname: Malpas
authors.forename: S M
authors.initials: SM
authors.lastname: Fitzgerald
authors.forename: A
authors.initials: A
authors.lastname: Shweta
authors.forename: F
authors.initials: F
authors.lastname: Tomoda
authors.forename: W P
authors.initials: WP
authors.lastname: Anderson
mesh_terms: D000818:Animals; D001783:Blood Flow Velocity; D004285:Dogs; D007668:Kidney / Q000098:blood supply*; D007677:Kidney Function Tests / Q000379:methods; D008297:Male; D019736:Prostheses and Implants; D012079:Renal Circulation / Q000502:physiology*; D015203:Reproducibility of Results; D012212:Rheology / Q000379:methods; D012826:Silicone Elastomers; D014463:Ultrasonography / Q000379:methods*; D010130:p-Aminohippuric Acid / Q000493:pharmacokinetics
chemical_list: D012826:Silicone Elastomers; D010130:p-Aminohippuric Acid