Studies of the hepatic mitochondrial and microsomal mixed-function oxidase system during Plasmodium yoelii infection and inducer treatment in Swiss albino mice.
Plasmodium yoelii infection resulted in depression of hepatic mitochondrial and microsomal mixed-function oxidase system indices, e.g. cytochrome P-450, cytochrome b5 and phase II detoxification enzyme glutathione-S-transferase, while heam and haemozoin registered a marked increase in Swiss albino mice. Phenobarbitone (inducer) treatment showed induced levels of hepatic mitochondrial and microsomal cytochrome P-450 and glutathione-S-transferase in normal as well as in infected mice. The induced cytochrome P-450 and glutathione-S-transferase activities were similar in normal and infected mice. The findings were further supported by the isoenzymic profile and drug-binding properties of the terminal monoxygenase, cytochrome P-450.
Srivastava
P
P
Department of Biochemistry, Central Drug Research Institute, Lucknow, India.
Sharma
S N
SN
Shukla
O P
OP
Pandey
V C
VC
D000818:Animals; D003577:Cytochrome P-450 Enzyme System / Q000378:metabolism; D018757:GABA Modulators / Q000627:therapeutic use; D008288:Malaria / Q000188:drug therapy / Q000201:enzymology*; D051379:Mice; D008862:Microsomes, Liver / Q000201:enzymology*; D008930:Mitochondria, Liver / Q000201:enzymology*; D006899:Mixed Function Oxygenases / Q000378:metabolism*; D010634:Phenobarbital / Q000627:therapeutic use; D010967:Plasmodium yoelii
D018757:GABA Modulators; D003577:Cytochrome P-450 Enzyme System; D006899:Mixed Function Oxygenases; D010634:Phenobarbital