Detection of the circulating lung cancer marker LCAP with a new monoclonal antibody TRD-L1.
Lung Cancer Associated Protein (LCAP) is a high molecular weight glycoprotein defined by the monoclonal antibody (MAb) DF-L1 prepared against a primary adenocarcinoma of the lung. Previous studies have demonstrated that LCAP circulates at elevated levels in patients with lung cancer. However, a suitable assay for monitoring LCAP levels has not been available. The present work describes the development of a double-determinant LCAP assay using MAb TRD-L1 as the capture antibody and MAb DF-L1 as the tracer. In 60 normal subjects, the mean LCAP level was 4.8 U/ml with 2 (3.3%) subjects having values > 12 U/ml (mean + 2SDS). By contrast, 37 of 67 (55.2%) patients with lung cancer had LCAP levels > 12 U/ml. Moreover, only 14 of 203 (6.9%) patients with benign lung disease had elevated levels. LCAP levels were most commonly elevated (62.7%) in patients with adenocarcinoma of the lung and with advanced disease. These results indicate that LCAP as detected by MAb TRD-L1 is a potentially useful marker for the evaluation of patients with lung cancer.
Tsuji
K
K
Research and Development Center, Terumo Corporation, Japan.
Uwano
T
T
Yoshida
Y
Y
Kikuchi
K
K
Okamoto
T
T
Yamamoto
S
S
Ichiki
M
M
Hayashi
I
I
Ohno
T
T
Kufe
D W
DW
D000230:Adenocarcinoma / Q000097:blood / Q000175:diagnosis / Q000473:pathology; D000911:Antibodies, Monoclonal; D014408:Biomarkers, Tumor / Q000097:blood*; D004797:Enzyme-Linked Immunosorbent Assay; D006023:Glycoproteins / Q000097:blood*; D006801:Humans; D008171:Lung Diseases / Q000097:blood; D008175:Lung Neoplasms / Q000097:blood / Q000175:diagnosis* / Q000473:pathology; D009367:Neoplasm Staging; D009369:Neoplasms / Q000097:blood; D012016:Reference Values; D012044:Regression Analysis; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
D000911:Antibodies, Monoclonal; D014408:Biomarkers, Tumor; D006023:Glycoproteins; C109072:lung cancer-associated protein, LCAP