A family of repetitive DNA sequences in Old World primates.
A dispersed family of repetitive DNA sequences that is amplified in Old World primates has been characterized. The sequences are present in about 250-350 copies in humans, found on all chromosomes, and some are at least 1 kb in size. Within the core repeat is a 178-bp region that is moderately-to-highly conserved. A representative sequence exhibited strong promoter activity when placed in front of a promoterless gene and transfected into human cells. This promoter activity has been localized to a 138-bp region of the repeat that is about 150 bp downstream of the 178-bp conserved region. Transcripts of the sequences were not detected in six human breast epithelial and teratocarcinoma cell lines. Based upon the work of Pavelitz et al. [Pavelitz, T., Rusche, L., Matera, A.G., Scharf, J.M., Weiner, A.M., 1995. EMBO J. 14, 169-177], the sequence appears to be related to the LTR of an HERV-K class human endogenous retrovirus.
Fanning
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Department of Cellular Pathology, Armed Forces Institute of Pathology, Washington, DC 20306-6000, USA. fanning@email.afip.osd.mil
Alves
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D000818:Animals; D001483:Base Sequence; D001943:Breast Neoplasms / Q000235:genetics; D002460:Cell Line; D002522:Chlorocebus aethiops / Q000235:genetics*; D003104:Colobus / Q000235:genetics*; D017124:Conserved Sequence / Q000235:genetics; D006801:Humans; D011401:Promoter Regions, Genetic / Q000235:genetics; D012333:RNA, Messenger / Q000032:analysis; D012091:Repetitive Sequences, Nucleic Acid / Q000235:genetics*; D017422:Sequence Analysis, DNA
D012333:RNA, Messenger