title: Assessment of human long saphenous vein function with minimally invasive harvesting with the Mayo stripper.
abstract: BACKGROUND
  The use of the Mayo Stripper to harvest the long saphenous vein has been shown to improve morbidity from leg wound incisions. It has not been universally accepted because of a perceived increase in injury to the venous conduit.
  
  
  OBJECTIVE
  To compare the function of undistended autologous long saphenous vein harvested by a Mayo stripper with the traditional 'open' technique in the same patient (n = 12) appearance.
  
  
  METHODS
  Vascular reactivity was assessed in isolated organ baths. Contractile function was measured in response to increasing concentrations (10(-9)-10(-5) mol) of 5-hydroxytryptamine and noradrenaline. This was calculated as a percentage of the maximum contractile response to 90 mM KCl measured in millinewtons (mN) (control 41.4 +/- 12.1, (n = 11), open technique 35.8 +/- 11.1, (n = 11), Mayo stripper 33.7 +/- 15.9, (n = 11)). The endothelial dependent and independent function was assessed with acetylcholine and sodium nitroprusside, respectively.
  
  
  RESULTS
  There was no significant difference in response to both constrictors and dilators between vein taken with the Mayo stripper compared with the traditional open technique (n = 6 for each observation; P > 0.05 by ANOVA). Histological examination by light microscopy of the vessel segments removed with the Mayo stripper was unable to show any significant damage to the vessel wall. Both functional and morphological studies were conducted by 'blinded' observers. One-year follow-up with magnetic resonance angiography (MRA) and stress thallium tomography demonstrated a patency rate with lower and upper estimates of 80 and 94%.
  
  
  CONCLUSIONS
  We have shown that harvesting the long saphenous vein with a Mayo stripper does not compromise vascular reactivity of the long saphenous vein or long-term patency.
authors.affiliation: Royal Brompton Hospital, National Heart and Lung Institute, London, UK.
authors.forename: D J
authors.initials: DJ
authors.lastname: O'Regan
authors.forename: J A
authors.initials: JA
authors.lastname: Borland
authors.forename: A H
authors.initials: AH
authors.lastname: Chester
authors.forename: D J
authors.initials: DJ
authors.lastname: Pennell
authors.forename: M
authors.initials: M
authors.lastname: Yacoub
authors.forename: J R
authors.initials: JR
authors.lastname: Pepper
mesh_terms: D000704:Analysis of Variance; D001026:Coronary Artery Bypass; D004210:Dissection / Q000379:methods*; D005500:Follow-Up Studies; D006801:Humans; D008875:Middle Aged; D019060:Minimally Invasive Surgical Procedures / Q000295:instrumentation* / Q000379:methods*; D009119:Muscle Contraction / Q000187:drug effects; D009131:Muscle, Smooth, Vascular / Q000187:drug effects; D012501:Saphenous Vein / Q000187:drug effects / Q000502:physiology* / Q000601:surgery*; D016037:Single-Blind Method; D014662:Vasoconstrictor Agents / Q000494:pharmacology; D014665:Vasodilator Agents / Q000494:pharmacology
chemical_list: D014662:Vasoconstrictor Agents; D014665:Vasodilator Agents