Production of poly(3-hydroxybutyrate-co-4-hydroxybutyrate) in recombinant Escherichia coli grown on glucose.
A recombinant Escherichia coli strain has been developed that produces poly(3-hydroxybutyrate-co-4-hydroxybutyrate) when grown in complex medium containing glucose. This has been accomplished by introducing into E. coli DH5 alpha separate plasmids harboring the polyhydroxyalkanoate (PHA) biosynthesis genes from Ralstonia eutropha (formerly named Alcaligenes eutrophus) and the succinate degradation genes from Clostridium kluyveri, respectively. Poly(3-hydroxybutyrate-co-4-hydroxybutyrate) levels reached 50% of the cell dry weight and contained up to 2.8 mol.% 4-hydroxybutyrate. The molecular weight of the polymer was 1.8 x 10(6).
Valentin
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HE
Department of Biology, James Madison University, Harrisonburg, VA 22807, USA. hevale@ccmail.monsanto.com
Dennis
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D000217:Acyltransferases / Q000235:genetics*; D000421:Alcaligenes / Q000235:genetics; D002849:Chromatography, Gas; D004926:Escherichia coli / Q000235:genetics*; D005947:Glucose / Q000494:pharmacology*; D006885:Hydroxybutyrates / Q000378:metabolism*; D009682:Magnetic Resonance Spectroscopy; D008970:Molecular Weight; D011091:Polyesters / Q000378:metabolism*; D000094122:Polyhydroxybutyrates
D000217:Acyltransferases; D005947:Glucose; D006885:Hydroxybutyrates; D011091:Polyesters; C003182:poly-beta-hydroxybutyrate; D000094122:Polyhydroxybutyrates; C077225:poly(3-hydroxyalkanoic acid) synthase