Preliminary NMR studies of Thermus thermophilus ribosomal protein S19 overproduced in Escherichia coli.
The gene for the ribosomal protein S19 from Thermus thermophilus was cloned, sequenced and overexpressed in Escherichia coli. A simple procedure for isolating the recombinant protein was developed. Preliminary NMR studies revealed a high content of alpha-helical secondary structure in the protein.
Davydova
N L
NL
Institute of Protein Research, Russian Academy of Sciences, Pushchino, Moscow Region.
Rak
A V
AV
Gryaznova
O I
OI
Liljas
A
A
Jonsson
B H
BH
Berglund
H
H
Härd
T
T
Garber
M B
MB
D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000737:chemistry* / Q000235:genetics; D003001:Cloning, Molecular; D017931:DNA Primers; D004926:Escherichia coli / Q000235:genetics; D015870:Gene Expression; D009682:Magnetic Resonance Spectroscopy; D008969:Molecular Sequence Data; D017510:Protein Folding; D017433:Protein Structure, Secondary; D011994:Recombinant Proteins / Q000737:chemistry; D012269:Ribosomal Proteins / Q000737:chemistry* / Q000235:genetics; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D016963:Thermus thermophilus / Q000737:chemistry / Q000235:genetics*
D001426:Bacterial Proteins; D017931:DNA Primers; D011994:Recombinant Proteins; D012269:Ribosomal Proteins; C040343:ribosomal protein S19