In situ hybridisation mapping of genomic clones for five human respiratory chain complex I genes.
Using human and bovine short cDNA sequences as probes we screened human cosmid and P1 libraries for components of the complex I multi-subunit enzyme of oxidative phosphorylation. We isolated genomic recombinants encoding cI-B8 (gene NDUFA2), cI-B14 (gene NDUFA6), cI-B14.5a (gene NDUFA7), cI-ASHI (gene NDUFB8) and cI-23kD (gene NDUFS8). Genomic versions of these genes have not been previously cloned in the human although they are represented as anonymous entries in public cDNA databases. By using the derived genomic clones for in situ hybridisation studies we determined the following chromosome locations: NDUFA2, 5q31; NDUFA6, 21q22; NDUFA7, 20p13; NDUFB8, 12q21; NDUFS8, 3q28.
Dunbar
D R
DR
MRC Human Genetics Unit, Western General Hospital, Edinburgh, UK.
Shibasaki
Y
Y
Dobbie
L
L
Andersson
B
B
Brookes
A J
AJ
D000818:Animals; D002417:Cattle; D002874:Chromosome Mapping; D003360:Cosmids; D018076:DNA, Complementary; D004579:Electron Transport / Q000235:genetics*; D008040:Genetic Linkage; D015894:Genome, Human; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D009154:Mutation; D016660:NAD(P)H Dehydrogenase (Quinone) / Q000235:genetics*; D016133:Polymerase Chain Reaction
D018076:DNA, Complementary; D016660:NAD(P)H Dehydrogenase (Quinone)