title: On the recovery of single spots with the flr phenotype in the wing spot test in Drosophila.
abstract: Graf et al. (U. Graf, F.E. Würgler, A.J. Katz, H. Frei, H. Juon, C.B. Hall, P.G. Kale, Somatic mutation and recombination test in Drosophila, Environment Mutagen. 6 (1984) 153-188.) described the overall results of assays of a series of compounds in the Drosophila wing spot test as indicating that single mwh spots appeared most frequently, followed by less frequent twin spots with both mwh and flr cells and lastly the 'quite rare' single flr spots. Data are presented below demonstrating that some compounds behave in a manner consistent with the above description, whereas others do not in that the frequency of single flr spots is equal to or exceeds that of twin spots and cannot be described as occurring 'rarely'. It is suggested that (large) flr singles be used as a measure of mutations/deletions directly from treated transheterozygotes. An argument is presented questioning the use of treated mwh +/+ TM3 individuals as an assay of mutations/deletions at the mwh+ locus.
authors.affiliation: Program in Biology, Brown University, Providence, RI 02912, USA.
authors.forename: S
authors.initials: S
authors.lastname: Zimmering
authors.forename: M P
authors.initials: MP
authors.lastname: Cruces
authors.forename: E
authors.initials: E
authors.lastname: Pimentel
authors.forename: C
authors.initials: C
authors.lastname: Arceo
authors.forename: G
authors.initials: G
authors.lastname: Carrasco
authors.forename: O
authors.initials: O
authors.lastname: Olvera
mesh_terms: D000818:Animals; D017608:Chromium Compounds; D004331:Drosophila melanogaster / Q000235:genetics*; D005020:Ethyl Methanesulfonate; D005038:Ethylnitrosourea; D005260:Female; D005720:Gamma Rays; D008297:Male; D008770:Methylnitrosourea; D009152:Mutagenicity Tests; D009153:Mutagens; D010641:Phenotype; D014921:Wings, Animal / Q000187:drug effects / Q000528:radiation effects; D014965:X-Rays
chemical_list: D017608:Chromium Compounds; D009153:Mutagens; D008770:Methylnitrosourea; D005020:Ethyl Methanesulfonate; D005038:Ethylnitrosourea; C023600:chromic oxide