Amitriptyline: a potent inhibitor of butyrylcholinesterase from human serum.
1. The effect of amitriptyline on human serum butyrylcholinesterase (acylcholine acylhydrolase E.C.3.1.1.8) has been investigated. From the Lineweaver-Burk plot and the plot of v versus amitriptyline concentration, it was concluded that amitriptyline inhibition is partially competitive, and the kinetic parameters have been calculated as Ks = 0.11 mM, alpha = 1425 and Ki = 0.01 mM. 2. Because amitriptyline is a partial competitive inhibitor of butyrylcholinesterase, acquired deficiency may be seen in patients treated with amitriptyline and may cause complications in operations.
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Hacettepe University, Faculty of Medicine, Department of Biochemistry, Ankara, Turkey.
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D018759:Adrenergic Uptake Inhibitors / Q000494:pharmacology*; D000639:Amitriptyline / Q000494:pharmacology*; D002091:Butyrylcholinesterase / Q000097:blood*; D002800:Cholinesterase Inhibitors / Q000494:pharmacology*; D006801:Humans; D007700:Kinetics; D009419:Nerve Tissue Proteins / Q000378:metabolism; D012044:Regression Analysis; D013056:Spectrophotometry, Ultraviolet
D018759:Adrenergic Uptake Inhibitors; D002800:Cholinesterase Inhibitors; D009419:Nerve Tissue Proteins; D000639:Amitriptyline; D002091:Butyrylcholinesterase