Simplified, rapid diagnosis of respiratory syncytial virus from clinical specimens.
DOT ELISA was compared with RT-PCR and tissue culture to detect RSV from nasopharyngeal aspirates. DOT ELISA had diagnostic sensitivity and specificity of 65.62% and 93.92%, respectively. The results indicate that DOT ELISA can be used for screening detection of RSV from clinical specimens and is suitable for small laboratories in the provincial areas of developing countries.
Tantivanich
S
S
Department of Microbiology and Immunology, Faculty of Tropical Medicine, Mahidol University, Bangkok, Thailand.
Klongkamnuankarn
K
K
Desakorn
V
V
D002478:Cells, Cultured; D002648:Child; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D006801:Humans; D007223:Infant; D017817:Nasal Lavage Fluid / Q000821:virology; D016133:Polymerase Chain Reaction / Q000379:methods*; D018357:Respiratory Syncytial Virus Infections / Q000175:diagnosis*; D012136:Respiratory Syncytial Viruses / Q000254:growth & development / Q000302:isolation & purification*; D012680:Sensitivity and Specificity