title: Group A streptococcal outbreak of perianal infection in a day-care centre.
abstract: Within 9 days in a day-care centre of 20 children 3 cases and 2 suspected cases of perianal infection (PAI) were revealed. The causative agent for PAI was confirmed to be beta-haemolytic group A streptococcus (GAS). The isolates were further characterized by T- and M-serotyping, conventional restriction endonuclease analysis (REA) and pulsed field gel electrophoresis (PFGE). The typing methods revealed that all outbreak isolates were of same T28R28-serotype and genetically identical. Unrelated, control GAS isolates of the same serotype differed genetically from the outbreak isolates indicating that a single clone had caused the perianal infections.
authors.affiliation: National Public Health Institute, Department of Bacteriology, Helsinki, Finland.
authors.forename: A
authors.initials: A
authors.lastname: Muotiala
authors.forename: H
authors.initials: H
authors.lastname: Saxén
authors.forename: J
authors.initials: J
authors.lastname: Vuopio-Varkila
mesh_terms: D001004:Anus Diseases / Q000453:epidemiology* / Q000382:microbiology; D002656:Child Day Care Centers; D002675:Child, Preschool; D004269:DNA, Bacterial / Q000235:genetics / Q000302:isolation & purification; D004196:Disease Outbreaks; D016521:Electrophoresis, Gel, Pulsed-Field; D005387:Finland / Q000453:epidemiology; D005838:Genotype; D006801:Humans; D008297:Male; D000091402:Prohibitins; D016036:Seroepidemiologic Studies; D012703:Serotyping; D013290:Streptococcal Infections / Q000453:epidemiology* / Q000382:microbiology; D013297:Streptococcus pyogenes / Q000145:classification / Q000235:genetics / Q000302:isolation & purification
chemical_list: D004269:DNA, Bacterial; C000717331:PHB2 protein, human; D000091402:Prohibitins