Positioning of 72 potentially full size LTRs of human endogenous retroviruses HERV-K on the human chromosome 19 map. Occurrences of the LTRs in human gene sites.
Seventy-two near full size long terminal repeats (LTRs) of human endogenous retrovirus of K-family (HERV-K) have been precisely located on the metric map of human chromosome 19. The LTR-related sequences were identified and assigned to cosmids by hybridization with two independent chromosome 19 specific cDNA clones corresponding to different parts of U3 region of LTR of HERV-K. The presence of full-size LTR sequences in a cosmid was further verified by PCR assay with a pair of primers complementary to the termini of the LTR. Coincidences of the LTR and the known genes positions are discussed.
Vinogradova
T
T
Shemyakin Institute of Bioorganic Chemistry, Russian Academy of Science, Miklukho-Maklaya, Moscow. tv@genome.siobc.ras.ru
Volik
S
S
Lebedev
Y u
Yu
Shevchenko
Y u
Yu
Lavrentyeva
I
I
Khil
P
P
Grzeschik
K H
KH
Ashworth
L K
LK
Sverdlov
E
E
D001483:Base Sequence; D002874:Chromosome Mapping; D002888:Chromosomes, Human, Pair 19 / Q000235:genetics* / Q000821:virology; D003360:Cosmids / Q000235:genetics; D005796:Genes / Q000235:genetics; D006801:Humans; D008969:Molecular Sequence Data; D012091:Repetitive Sequences, Nucleic Acid / Q000235:genetics*; D012190:Retroviridae / Q000235:genetics*; D012689:Sequence Homology, Nucleic Acid