title: Comparison of the molecular, antigenic and ATPase determinants of fast myosin heavy chains in rat and human: a single-fibre study.
abstract: Combined methodologies of histochemistry, immunohistochemistry, sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE), reverse transcriptase polymerase chain reaction (RT-PCR) and a histochemical method specific for myofibrillar ATPase (mATPase) of the type IIX myosin heavy chain (MyHC) isoform were used to study human and rat single fibres to examine the homology between type II MyHC isoform-based fibres of both species. We demonstrate that human type II fibres exhibit antigenic mATPase and 3'-untranslated region (3'-UTR) sequence determinants homologous to the IIA and IIX but not the IIB MyHC isoforms of the rat. Both immunolabelling with anti-MyHC monoclonal antibodies and the mATPase method used with frozen sections confirmed that all human type II fibres express type IIA and/or type IIX MyHC. Quantitative immunohistochemistry failed to recognize human fibres with antigenic characteristics corresponding to hybrid IIXB MyHC-based fibres. Ca2+-stimulated maximum myosin ATPase activity, determined by quantitative histochemistry, revealed that human IIX fibres (with an optical density or OD = 0.707) display enzyme activity which is comparable to that of the rat type IIX (OD = 0.687) but lower than that of the rat type IIB fibres (OD = 0.836). The results do not support the notion that MyHC IIB is expressed in human limb muscles, even in hybrid fibres. We conclude that human type II fibres have been misclassified in numerous previous publications and that this has important implications in attempts to compare the physiological characteristics of fibre types, particularly when animal models are used.
authors.affiliation: Department of Anatomy and Developmental Biology, The Royal Free Hospital School of Medicine, Division of Biomedical Sciences, Rowland Hill Street, Hampstead, NW3 2PF, London, UK.
authors.forename: J A
authors.initials: JA
authors.lastname: Pereira Sant'Ana
authors.forename: S
authors.initials: S
authors.lastname: Ennion
authors.forename: A J
authors.initials: AJ
authors.lastname: Sargeant
authors.forename: A F
authors.initials: AF
authors.lastname: Moorman
authors.forename: G
authors.initials: G
authors.lastname: Goldspink
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal; D001483:Base Sequence; D002118:Calcium / Q000494:pharmacology; D004591:Electrophoresis, Polyacrylamide Gel; D006651:Histocytochemistry; D006801:Humans; D007150:Immunohistochemistry; D008297:Male; D008969:Molecular Sequence Data; D018656:Muscle Fibers, Fast-Twitch / Q000201:enzymology*; D018995:Myosin Heavy Chains / Q000032:analysis*; D009218:Myosins / Q000032:analysis*; D016133:Polymerase Chain Reaction; D012194:RNA-Directed DNA Polymerase; D051381:Rats; D017208:Rats, Wistar; D016415:Sequence Alignment; D013045:Species Specificity
chemical_list: D000911:Antibodies, Monoclonal; D012194:RNA-Directed DNA Polymerase; D018995:Myosin Heavy Chains; D009218:Myosins; D002118:Calcium