High responsiveness to thyroid hormone of adult rat primary hepatocytes cultured on EHS-gel.
The induction of malic enzyme gene expression by triiodothyronine and insulin was severely blunted in rat monolayer hepatocytes cultured on type I collagen compared with that in spherical hepatocytes cultured on a reconstituted basement membrane gel (EHS-gel). Although the mRNA level of thyroid hormone receptor beta (TR beta) gradually decreased in the monolayer hepatocytes during culture, the mRNA level in the hepatocytes on EHS-gel was maintained at around the in vivo level. Our results suggest that the maintenance of TR beta mRNA on EHS-gel is responsible for the high responsiveness to thyroid hormone in a hepatocyte culture.
Oda
H
H
Department of Applied Biological Sciences, Nagoya University, Japan. hirooda@agr.nagoya-u.ac.jp
Nozawa
K
K
Miyachi
F
F
Shimizu
A
A
Iwasaki
Y
Y
Kakinuma
A
A
D000818:Animals; D002478:Cells, Cultured; D003094:Collagen / Q000096:biosynthesis; D003470:Culture Media; D008099:Liver / Q000166:cytology / Q000187:drug effects* / Q000201:enzymology; D008291:Malate Dehydrogenase / Q000096:biosynthesis; D008297:Male; D012333:RNA, Messenger / Q000096:biosynthesis; D051381:Rats; D017208:Rats, Wistar; D011988:Receptors, Thyroid Hormone / Q000096:biosynthesis; D013963:Thyroid Hormones / Q000494:pharmacology*
D003470:Culture Media; D012333:RNA, Messenger; D011988:Receptors, Thyroid Hormone; D013963:Thyroid Hormones; D003094:Collagen; D008291:Malate Dehydrogenase