Immunoregulation via adhesion molecules in allogenic and xenogenic hepatocyte transplantation to Nagase's analbuminemic rats.
We investigated the effects of monoclonal antibodies (mAbs) against lymphocyte function-associated antigen-1 (LFA-1) and intercellular adhesion molecule-1 (ICAM-1) on intrasplenic allogenic and xenogenic hepatocyte transplantation (HCTx) to analbuminemic rats. Ten to 12-wk-old male Nagase's analbuminemic rats (RT1l) were used as recipients, Wistar/Shi rats (RT1k) were used as donors for allografts and BALB/C mice were used as donors for xenografts. The experimental groups were as follows: group 1, allo-HCTx (n = 7); group 2, allo-HCTx + antirat ICAM-1/antirat LFA-1 mAbs (1.0 mg/kg/day, for 7 days, respectively) (n = 6); group 3, xeno-HCTx (n = 5); group 4, xeno-HCTx + mAbs (antimouse LFA-1/antirat ICAM-1) (n = 5). group 5, xeno-HCTx + mAbs (antirat LFA-1/antimouse ICAM-1) (n = 5). Serum rat albumin levels were measured in groups 1 and 2, and serum mouse albumin levels were measured in groups 3, 4, and 5, as indicators of the function of grafted hepatocytes. In allotransplantation groups, the serum rat albumin levels in the mAbs-treated group were significantly higher than those in the control group for 6 wk after transplantation. In xenotransplantation groups, no increase in the serum mouse albumin levels was detected in any group
Horimoto
H
H
Department of Surgery, Meikai University, Japan.
Nozawa
M
M
Kokudo
N
N
Nakao
M
M
Takahashi
S
S
Miyasaka
M
M
Seino
K
K
Yagita
H
H
Okumura
K
K
D000818:Animals; D000911:Antibodies, Monoclonal; D017690:Cell Transplantation; D006084:Graft Rejection / Q000276:immunology*; D018799:Intercellular Adhesion Molecule-1 / Q000502:physiology*; D008099:Liver / Q000166:cytology*; D016169:Lymphocyte Function-Associated Antigen-1 / Q000502:physiology*; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D051381:Rats; D011922:Rats, Mutant Strains; D017208:Rats, Wistar; D012709:Serum Albumin / Q000378:metabolism; D013154:Spleen; D014183:Transplantation, Heterologous; D016042:Transplantation, Heterotopic; D014184:Transplantation, Homologous
D000911:Antibodies, Monoclonal; D016169:Lymphocyte Function-Associated Antigen-1; D012709:Serum Albumin; D018799:Intercellular Adhesion Molecule-1