title: Nisin production by Lactococcus lactis using two-phase batch culture.
abstract: Nisin production by Lactococcus lactis subsp. lactis C2SmPrt-(TnNip) was investigated using two-phase batch culture. A solvent (phenyl-methyl silicone oil) was introduced in addition to the aqueous phase. The partition coefficient of this solvent in aqueous nisin varied as the cultivation medium changed, with a minimum value being 1.04. The two-phase batch culture supported a 21% increase in growth and a 24% increase in the level of nisin produced compared to the single-phase batch culture.
authors.affiliation: Department of Biotechnology, University of New South Wales, Sydney, Australia.
authors.forename: W S
authors.initials: WS
authors.lastname: Kim
mesh_terms: D001431:Bacteriological Techniques; D001709:Biotechnology; D013294:Lactococcus lactis / Q000254:growth & development / Q000378:metabolism*; D009561:Nisin / Q000096:biosynthesis* / Q000302:isolation & purification; D012997:Solvents; D014867:Water
chemical_list: D012997:Solvents; D014867:Water; D009561:Nisin