Temporal regulation of sigD from Bacillus subtilis depends on a minor promoter in front of the gene.
I investigated the transcriptional regulation of sigmaD synthesis. sigD is part of the fla/che operon, but the gene is also preceded by a promoter of its own. fla/che-dependent transcription is severely reduced in sigD-negative strains. Activity of the promoter in front of sigD is strictly temporally regulated. Deletion of this promoter results in a reduced and delayed activation of transcription of the fla/che operon.
Allmansberger
R
R
Lehrstuhl für Mikrobiologie, Universität Erlangen-Nürnberg, Erlangen, Germany. rallmans@biologie.uni-erlangen.de
D001412:Bacillus subtilis / Q000235:genetics* / Q000502:physiology; D002633:Chemotaxis; D012321:DNA-Directed RNA Polymerases / Q000096:biosynthesis / Q000235:genetics*; D015964:Gene Expression Regulation, Bacterial; D009068:Movement; D009876:Operon; D011401:Promoter Regions, Genetic; D012808:Sigma Factor / Q000096:biosynthesis / Q000235:genetics*
D012808:Sigma Factor; C081775:RNA polymerase sigma 70; D012321:DNA-Directed RNA Polymerases