title: Role of gastrin/CCK-B receptor in the regulation of gastric acid secretion in rat.
abstract: The aim of this study was to investigate whether gastrin regulates morphological changes and alpha-subunit gene expression in parietal cells through the gastrin/CCK-B receptor on enterochromaffin-like cells by histamine release. Treatment with 100 mg/kg of YM022, a potent and selective gastrin/CCK-B receptor antagonist, for one week in rats did not alter mRNA levels of histidine decarboxylase or H+, K+-ATPase. However, parietal cell morphology predominantly changed to the resting form, although the serum gastrin concentration was significantly increased. Additional treatment with YM022 and oral omeprazole, 100 mg/kg, for one week markedly suppressed the increases of mRNA levels of histidine decarboxylase and H+, K+-ATPase and completely blocked the morphological transformation of the parietal cells to a stimulated form induced by treatment with omeprazole alone. This indicates that the morphological transformation of parietal cells to an activated form with a subsequent increase in H+, K+-ATPase synthesis caused by hypergastrinemia is mediated by increased histidine decarboxylase gene expression in enterochromaffin-like cells via gastrin/CCK-B receptors.
authors.affiliation: Department of Internal Medicine, Hiroshima Red Cross & Atomic-bomb Survivors Hospital, Japan.
authors.forename: A
authors.initials: A
authors.lastname: Tari
authors.forename: T
authors.initials: T
authors.lastname: Kamiyasu
authors.forename: Y
authors.initials: Y
authors.lastname: Yonei
authors.forename: M
authors.initials: M
authors.lastname: Hamada
authors.forename: M
authors.initials: M
authors.lastname: Sumii
authors.forename: K
authors.initials: K
authors.lastname: Sumii
authors.forename: G
authors.initials: G
authors.lastname: Kajiyama
authors.forename: R
authors.initials: R
authors.lastname: Dimaline
mesh_terms: D000199:Actins / Q000096:biosynthesis; D000818:Animals; D001569:Benzodiazepines / Q000494:pharmacology; D004791:Enzyme Inhibitors / Q000494:pharmacology; D005744:Gastric Acid / Q000378:metabolism*; D005755:Gastrins / Q000097:blood / Q000502:physiology*; D015870:Gene Expression; D017506:H(+)-K(+)-Exchanging ATPase / Q000096:biosynthesis; D006636:Histamine Release; D006640:Histidine Decarboxylase / Q000037:antagonists & inhibitors / Q000096:biosynthesis; D006727:Hormone Antagonists / Q000494:pharmacology; D008297:Male; D009853:Omeprazole / Q000494:pharmacology; D010295:Parietal Cells, Gastric / Q000187:drug effects*; D054328:Proton Pump Inhibitors; D012333:RNA, Messenger / Q000235:genetics; D051381:Rats; D017208:Rats, Wistar; D011949:Receptors, Cholecystokinin / Q000037:antagonists & inhibitors / Q000502:physiology*
chemical_list: D000199:Actins; D004791:Enzyme Inhibitors; D005755:Gastrins; D006727:Hormone Antagonists; D054328:Proton Pump Inhibitors; D012333:RNA, Messenger; D011949:Receptors, Cholecystokinin; D001569:Benzodiazepines; C087260:YM 022; D017506:H(+)-K(+)-Exchanging ATPase; D006640:Histidine Decarboxylase; D009853:Omeprazole