title: Muscle-specific creatine kinase gene polymorphism and VO2max in the HERITAGE Family Study.
abstract: This study examined the association between a DNA polymorphism in the muscle-specific creatine kinase (CKMM) gene and VO2max in the sedentary state, as well as its response (deltaVO2max) to a standardized 20-wk endurance training program. The subjects were 160 biologically unrelated Caucasian parents (80 women, 80 men) and 80 biologically unrelated adult offspring of the HERITAGE Family Study. The CKMM polymorphism was detected by PCR and digestion with the NcoI restriction enzyme. VO2max was measured during maximal cycle ergometer tests. VO2max was 2119 +/- 45 mL x min(-1) (mean +/- SE) or 26 +/- 0.4 mL x kg(-1) x min(-1). Both sexes had a significant (P < 0.05) increase in the deltaVO2max (women = 283 +/- 20 mL x min[-1] and men = 363 +/- 25 mL x min[-1]). Allele and genotype frequencies were not significantly different (P > 0.05) between sexes. Age and sex adjusted VO2max was significantly (P = 0.007) associated with the CKMM genotype in the parents, whereas no association (P > 0.05) was observed in the offspring. DeltaVO2max values adjusted for age, sex, VO2max, and body mass were characterized by genotype differences in both parents (P = 0.0004) and offspring (P = 0.0025). A significantly (P < 0.05) lower deltaVO2max to endurance training was detected in both parents and offspring homozygotes for the rare allele. The genotype accounted for at least 9% of the variance in deltaVO2max. These results indicate that the NcoI polymorphism in the 3' untranslated region of the muscle-specific creatine kinase gene is associated with the deltaVO2max to endurance training.
authors.affiliation: Physical Activity Sciences Laboratory, Laval University, Ste-Foy, Québec, Canada.
authors.forename: M A
authors.initials: MA
authors.lastname: Rivera
authors.forename: F T
authors.initials: FT
authors.lastname: Dionne
authors.forename: J A
authors.initials: JA
authors.lastname: Simoneau
authors.forename: L
authors.initials: L
authors.lastname: Pérusse
authors.forename: M
authors.initials: M
authors.lastname: Chagnon
authors.forename: Y
authors.initials: Y
authors.lastname: Chagnon
authors.forename: J
authors.initials: J
authors.lastname: Gagnon
authors.forename: A S
authors.initials: AS
authors.lastname: Leon
authors.forename: D C
authors.initials: DC
authors.lastname: Rao
authors.forename: J S
authors.initials: JS
authors.lastname: Skinner
authors.forename: J H
authors.initials: JH
authors.lastname: Wilmore
authors.forename: C
authors.initials: C
authors.lastname: Bouchard
mesh_terms: D000328:Adult; D000483:Alleles; D000704:Analysis of Variance; D016009:Chi-Square Distribution; D003402:Creatine Kinase / Q000235:genetics*; D005260:Female; D014644:Genetic Variation; D005838:Genotype; D006801:Humans; D016014:Linear Models; D008297:Male; D008875:Middle Aged; D018482:Muscle, Skeletal / Q000201:enzymology*; D010101:Oxygen Consumption / Q000235:genetics*; D010806:Physical Education and Training; D010807:Physical Endurance / Q000502:physiology*; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic
chemical_list: D003402:Creatine Kinase