The effect of intravenous administration of WEB 2086 on PAF-induced platelet aggregation in healthy Friesian calves.
The in vivo ability of the specific PAF-antagonist WEB 2086, a thienotriazolodiazepine, to inhibit platelet-activating factor (PAF) in cattle was investigated by in vitro determination of platelet aggregation curves. WEB 2086 was infused intravenously into a group of 5 healthy male Friesian calves in a dose of 3 mg/kg over 1 min. The resultant inhibition peaked between 30 min and 1 h after administration of WEB 2086. The inhibition was significantly reduced after 3 h and became non-significant after 6 h, but maximal pre-treatment aggregation had not been restored by 24 h after the injection of WEB 2086. These results confirm previous results obtained in vitro and suggest that WEB 2086 is a potent antagonist of PAF activity in calves. They also suggest that further clinical studies with WEB 2086 in cattle are desirable.
Bastos da Silva
M B
MB
Department of Physiology, University of Liege, Sart Tilman, Belgium.
Gustin
P
P
Herion
F
F
Raskinet
R
R
David
J L
JL
Gougnard
T
T
Plomteux
G
G
Desmecht
D
D
Lekeux
P
P
D000818:Animals; D001381:Azepines / Q000008:administration & dosage / Q000494:pharmacology*; D002417:Cattle / Q000097:blood* / Q000502:physiology; D002851:Chromatography, High Pressure Liquid / Q000379:methods / Q000662:veterinary; D007262:Infusions, Intravenous / Q000662:veterinary; D008297:Male; D010972:Platelet Activating Factor / Q000494:pharmacology*; D010974:Platelet Aggregation / Q000187:drug effects* / Q000502:physiology; D010975:Platelet Aggregation Inhibitors / Q000008:administration & dosage / Q000494:pharmacology*; D013997:Time Factors; D014230:Triazoles / Q000008:administration & dosage / Q000494:pharmacology*
D001381:Azepines; D010972:Platelet Activating Factor; D010975:Platelet Aggregation Inhibitors; D014230:Triazoles; C052518:WEB 2086