Selective in vitro removal of anti-A antibodies by adsorption on encapsulated erythrocyte-ghosts.
Large volume plasma exchanges are used for the removal of anti-A or anti-B antibodies from the plasma of patients undergoing transplantation from donors with major ABO incompatibility. Previous works suggest that solid-phase immunoadsorption can be substituted for plasma exchange in situations where antigens can be purified and immobilized on columns through which plasma is percolated. However, the preparation of purified antigens of the ABO system is large quantities is laborious and requires the use of considerable blood volumes. Studies were therefore undertaken to determine the feasibility of an original immunoadsorbent based on porous microparticles prepared by a water/oil/water emulsification-solvent evaporation method, within which erythrocytes-ghosts carrying blood group antigens were entrapped. The decrease of the antibody hemagglutinating titre after adsorption onto encapsulated ghosts suggests that antibodies can cross the polymeric membrane and bind to the antigens. This original approach of using encapsulated antigens for the batchwise removal of antibodies could be extended to affinity chromatography, and immunoadsorption therapy with a chromatographic column linked to an extracorporeal circulation could be considered.
Ubrich
N
N
Laboratoire de Pharmacie Galénique et Biopharmacie, Faculté des Sciences Pharmaceutiques et Biologiques, Nancy, France.
Ngondi
J
J
Rivat
C
C
Pfister
M
M
Vigneron
C
C
Maincent
P
P
D000017:ABO Blood-Group System / Q000276:immunology*; D000327:Adsorption; D000906:Antibodies / Q000302:isolation & purification*; D002482:Cellulose / Q000031:analogs & derivatives; D004910:Erythrocyte Membrane / Q000276:immunology*; D006386:Hemagglutination Tests; D006801:Humans; D008855:Microscopy, Electron, Scanning; D008863:Microspheres; D010956:Plasmapheresis
D000017:ABO Blood-Group System; D000906:Antibodies; C013517:ethyl cellulose; D002482:Cellulose