title: Pseudomonas aeruginosa infection in embryonated hen's eggs. An alternative in vivo model for the screening of antibacterial substances.
abstract: Embryonated hens' eggs can be reliably infected by Pseudomonas aeruginosa in laboratory experiments. Therapy tests with the antibiotics azlocillin (CAS 37091-66-0) and gentamicin (CAS 13291-74-2) on this type of infected hens' eggs demonstrate that this test system offers a realistic alternative to septic experiments with small laboratory rodents. Chick embryos survive a lethal Pseudomonas infection when azlocillin or gentamicin in a relevant therapeutic dose are administered immediately after the infective agent. The use of Pseudomonas infected chick embryos in the screening for new antiinfectives allows, therefore, a considerable reduction of the number of laboratory rodents required.
authors.affiliation: Hans-Knöll-Institute of Natural Product Research e.V., Jena, Germany.
authors.forename: A
authors.initials: A
authors.lastname: Härtl
authors.forename: U
authors.initials: U
authors.lastname: Möllmann
authors.forename: E
authors.initials: E
authors.lastname: Schrinner
authors.forename: A
authors.initials: A
authors.lastname: Stelzner
mesh_terms: D000826:Animal Testing Alternatives; D000818:Animals; D000900:Anti-Bacterial Agents / Q000627:therapeutic use*; D001390:Azlocillin / Q000627:therapeutic use; D002642:Chick Embryo / Q000382:microbiology*; D004353:Drug Evaluation, Preclinical; D005839:Gentamicins / Q000627:therapeutic use; D011552:Pseudomonas Infections / Q000188:drug therapy* / Q000382:microbiology
chemical_list: D000900:Anti-Bacterial Agents; D005839:Gentamicins; D001390:Azlocillin