In vivo 1H-NMR microimaging with respiratory triggering for monitoring adoptive immunotherapy of metastatic mouse lymphoma.
The metastatic ESb-MP murine lymphoma in DBA/2 mice has been used as a model for investigating metastatic disease and its cure by adoptive immunotherapy (ADI) as monitored by in vivo multislice spin-echo 1H NMR microimaging at 7 T. isoflurane inhalation anesthesia facilitated long measurement sessions, and respiratory gating with a fiber-optic sensor greatly reduced motional artifacts. With T2 weighting (TR = 2 s, TE = 30 ms) mean signal-to-noise ratios of 30 and 15 for kidney and liver, respectively, were achieved in 20 min (100-micron pixels, 1-mm slices, 25-mm field of view). Without the use of contrast agents, metastases with diameters > or = 0.3 mm in the imaged plane could be detected as hyperintense lesions in kidney (contrast ratio ca. 1.4) and liver (contrast ratio ca. 2) with a confidence level of > 98%. For the first time the complete eradication of late-stage macroscopic metastases by ADI could be demonstrated noninvasively by MRI.
Fichtner
K P
KP
Central Spectroscopy Department, German Cancer Research Center, Heidelberg, Germany.
Schirrmacher
V
V
Griesbach
A
A
Hull
W E
WE
D000818:Animals; D016477:Artifacts; D004195:Disease Models, Animal; D018450:Disease Progression; D005260:Female; D005500:Follow-Up Studies; D016219:Immunotherapy, Adoptive; D007680:Kidney Neoplasms / Q000503:physiopathology / Q000556:secondary* / Q000628:therapy; D008113:Liver Neoplasms / Q000503:physiopathology / Q000556:secondary* / Q000628:therapy; D008175:Lung Neoplasms / Q000503:physiopathology / Q000556:secondary* / Q000628:therapy; D008223:Lymphoma / Q000175:diagnosis* / Q000503:physiopathology / Q000628:therapy; D009682:Magnetic Resonance Spectroscopy / Q000379:methods*; D051379:Mice; D008811:Mice, Inbred DBA; D012119:Respiration / Q000502:physiology*; D016896:Treatment Outcome