title: Concomitant and hormonally regulated expression of trp genes in bovine aortic endothelial cells.
abstract: Recent findings have suggested that the vertebrate trp family of channel proteins is the structural basis for Ca2+ influx through the capacitative calcium entry (CCE) pathway. We have discerned, in bovine aortic endothelial cells, the concomitant expression of four such vertebrate genes: trp-1 (two splice variants), trp-3, trp-4 and trp-5. Exogenous hormones rendered dynamic effects on the transcript levels of these genes. Most notably, beta-estradiol significantly down-regulated trp-4 while trans-retinoic acid dramatically up-regulated trp-5; yet these hormones rendered little change in CCE. These findings suggest that the extent of a given trp channel's participation in CCE is not reflected in alterations of its transcript level.
authors.affiliation: Rammelkamp Center for Research, MetroHealth Medical Center, Cleveland, OH 44109, USA. achang@research.mhmc.org
authors.forename: A S
authors.initials: AS
authors.lastname: Chang
authors.forename: S M
authors.initials: SM
authors.lastname: Chang
authors.forename: R L
authors.initials: RL
authors.lastname: Garcia
authors.forename: W P
authors.initials: WP
authors.lastname: Schilling
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001011:Aorta; D015139:Blotting, Southern; D002118:Calcium / Q000378:metabolism; D015220:Calcium Channels / Q000096:biosynthesis / Q000235:genetics*; D002417:Cattle; D002478:Cells, Cultured; D017931:DNA Primers; D004730:Endothelium, Vascular / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D004958:Estradiol / Q000494:pharmacology; D005786:Gene Expression Regulation / Q000187:drug effects*; D006728:Hormones / Q000494:pharmacology*; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D050052:TRPC Cation Channels; D014212:Tretinoin / Q000494:pharmacology
chemical_list: D015220:Calcium Channels; D017931:DNA Primers; D006728:Hormones; D050052:TRPC Cation Channels; C096351:transient receptor potential cation channel, subfamily C, member 1; D004958:Estradiol; D014212:Tretinoin; D002118:Calcium