Expression and function of endothelial cell alpha v integrin receptors in wound-induced human angiogenesis in human skin/SCID mice chimeras.
Accumulating evidence indicates that endothelial cell integrins that bind to the matrix proteins associated with inflammation and wound healing are involved in the process of angiogenesis. The integrins containing the alpha v subunit appear to be particularly important. To study the involvement of these receptors in human angiogenesis, a model of wound-associated human angiogenesis was established in human skin transplanted onto severe combined immunodeficient (SCID) mice. Using this model, we studied the expression of several alpha v integrins and tested the hypothesis that blockage of the alpha v beta 3 integrin would inhibit human angiogenesis during human wound healing. These studies revealed that the alpha v beta 3, alpha v beta 5, and alpha v beta 6 integrins are up-regulated briefly during wound angiogenesis with different patterns of expression and that inhibition of the alpha v beta 3 integrin blocked new vessel formation during human wound healing.
Christofidou-Solomidou
M
M
Department of Medicine, University of Pennsylvania Medical Center, Philadelphia 19104-4283, USA.
Bridges
M
M
Murphy
G F
GF
Albelda
S M
SM
DeLisser
H M
HM
D000818:Animals; D000911:Antibodies, Monoclonal / Q000494:pharmacology; D015703:Antigens, CD / Q000276:immunology / Q000378:metabolism*; D004730:Endothelium, Vascular / Q000378:metabolism*; D006801:Humans; D007124:Immunoenzyme Techniques; D007153:Immunologic Deficiency Syndromes / Q000378:metabolism*; D039564:Integrin alphaV; D051379:Mice; D016513:Mice, SCID; D009389:Neovascularization, Pathologic / Q000378:metabolism* / Q000517:prevention & control; D019408:Platelet Endothelial Cell Adhesion Molecule-1 / Q000032:analysis / Q000276:immunology; D012867:Skin / Q000098:blood supply / Q000293:injuries; D016038:Skin Transplantation; D019063:Tenascin / Q000032:analysis / Q000276:immunology; D018183:Transplantation Chimera; D015854:Up-Regulation / Q000502:physiology; D014945:Wound Healing
D000911:Antibodies, Monoclonal; D015703:Antigens, CD; D039564:Integrin alphaV; D019408:Platelet Endothelial Cell Adhesion Molecule-1; D019063:Tenascin