title: rRNA maturation as a "quality" control step in ribosomal subunit assembly in Dictyostelium discoideum.
abstract: In Dictyostelium discoideum, newly assembled ribosomal subunits enter polyribosomes while they still contain immature rRNA. rRNA maturation requires the engagement of the subunits in protein synthesis and leads to stabilization of their structure. Maturation of pre-17 S rRNA occurs only after the newly formed 40 S ribosomal particle has entered an 80 S ribosome and participated at least in the formation of one peptide bond or in one translocation event; maturation of pre-26 S rRNA requires the presence on the 80 S particle of a peptidyl-tRNA containing at least 6 amino acids. Newly assembled particles that cannot fulfill these requirements for structural reasons are disassembled into free immature rRNA and ribosomal proteins.
authors.affiliation: Department of Clinical and Biological Sciences, Ospedale S. Luigi, Orbassano, Torino, Italy.
authors.forename: G
authors.initials: G
authors.lastname: Mangiarotti
authors.forename: S
authors.initials: S
authors.lastname: Chiaberge
authors.forename: S
authors.initials: S
authors.lastname: Bulfone
mesh_terms: D000818:Animals; D004023:Dictyostelium / Q000235:genetics*; D012323:RNA Processing, Post-Transcriptional; D012331:RNA, Fungal / Q000378:metabolism*; D012335:RNA, Ribosomal / Q000378:metabolism*
chemical_list: D012331:RNA, Fungal; D012335:RNA, Ribosomal; C065096:RNA, ribosomal, 26S