title: Role of reactive oxygen intermediates in interleukin 10 release after cold liver ischemia and reperfusion in mice.
abstract: OBJECTIVE
  Reactive oxygen intermediates and cytokines are key effectors in reperfusion injury after liver ischemia. We hypothesized that reactive oxygen intermediates act as a signal for the release of tumor necrosis factor (TNF) and interleukin 10 (IL-10) after reperfusion of cold-preserved livers.
  
  
  METHODS
  An endotoxin-free isolated perfused mouse liver system was designed. Harvested mouse livers were stored at 4 degrees C for 0-28 hours and reperfused for 90 minutes with a warm oxygenated Hank's balanced salt solution (alone or with additives). Cytokine messenger RNA (mRNA) from whole liver was measured by reverse-transcription polymerase chain reaction. Cytokine protein levels and liver injury assessed by alanine aminotransferase levels were evaluated in liver effluent during reperfusion.
  
  
  RESULTS
  TNF and IL-10 mRNA and protein concentrations were increased after reperfusion of ischemic livers. N-Acetylcysteine and allopurinol dramatically decreased TNF (-64% and -62%) and IL-10 (-49% and -57%) levels in the effluents, as did an inhibitor of the transcription factor NF-kappaB mobilization (-73% and -76% for TNF and IL-10, respectively). Liver injury was decreased by -40%, -43%, and -54% for the three inhibitors, respectively.
  
  
  CONCLUSIONS
  Reactive oxygen intermediates are involved in TNF and IL-10 release after reperfusion of cold-preserved livers.
authors.affiliation: Department of Gastroenterology, Hôpital Erasme, Brussels, Belgium.
authors.forename: O
authors.initials: O
authors.lastname: Le Moine
authors.forename: H
authors.initials: H
authors.lastname: Louis
authors.forename: P
authors.initials: P
authors.lastname: Stordeur
authors.forename: J M
authors.initials: JM
authors.lastname: Collet
authors.forename: M
authors.initials: M
authors.lastname: Goldman
authors.forename: J
authors.initials: J
authors.lastname: Devière
mesh_terms: D000818:Animals; D003080:Cold Temperature; D005260:Female; D016753:Interleukin-10 / Q000378:metabolism* / Q000494:pharmacology; D007511:Ischemia / Q000378:metabolism*; D008099:Liver / Q000098:blood supply*; D051379:Mice; D008807:Mice, Inbred BALB C; D012333:RNA, Messenger / Q000032:analysis; D017382:Reactive Oxygen Species / Q000502:physiology*; D011994:Recombinant Proteins / Q000494:pharmacology; D015427:Reperfusion Injury / Q000209:etiology*; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism
chemical_list: D012333:RNA, Messenger; D017382:Reactive Oxygen Species; D011994:Recombinant Proteins; D014409:Tumor Necrosis Factor-alpha; D016753:Interleukin-10