Full length cDNA of rat RT1.DMa and RT1.DMb and expression of RT1.DM genes in dendritic and Langerhans cells.
MHC encoded DM heterodimers and classical MHC class II complexes meet in an endosomal/lysosomal compartment where DM heterodimers support peptide loading of MHC class II. Studies on peptide loading of rat class II and on peptide persistence in cells of the dendritic lineage prompted us to establish full length cDNA clones coding for the subunits alpha and beta of rat DM molecules as well as a mAb directed against the luminal moiety of the beta subunit. Here we describe the establishment of the first full length cDNA clones of rat RT1.DMa and RT1.DMb. The mode of expression of RT1.DM at the transcript level in bone marrow culture-derived dendritic cells, in Langerhans cells and in a number of additional accessory cells is reported. The beta protein was identified in detergent lysates of RT1.DM expressing cells by Western blot analysis using a newly established monoclonal antibody directed against the luminal part of RT1.DMbeta.
Kurth
D
D
Institut für Immunologie, Johannes Gutenberg Universität Mainz, Germany.
Neumann
J
J
Demleitner
K
K
Hildmann
J
J
Mehlig
M
M
Scheicher
C
C
Wendling
U
U
Sun
D
D
Reske
K
K
D000595:Amino Acid Sequence; D000818:Animals; D000911:Antibodies, Monoclonal; D001483:Base Sequence; D015152:Blotting, Northern; D015153:Blotting, Western; D001854:Bone Marrow Cells / Q000378:metabolism; D002478:Cells, Cultured; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000032:analysis*; D003713:Dendritic Cells / Q000378:metabolism*; D015870:Gene Expression; D005802:Genes, MHC Class II / Q000235:genetics* / Q000276:immunology; D006649:Histocompatibility Antigens / Q000737:chemistry / Q000235:genetics*; D007515:Islets of Langerhans / Q000378:metabolism*; D008297:Male; D008969:Molecular Sequence Data; D051381:Rats; D011917:Rats, Inbred Lew; D014158:Transcription, Genetic / Q000235:genetics
D000911:Antibodies, Monoclonal; D018076:DNA, Complementary; D006649:Histocompatibility Antigens; C038353:histocompatibility antigens RT, rat