title: Site-directed mutagenesis: a two-step method using PCR and DpnI.
authors.affiliation: Department of Immunology, University of Texas M.D. Anderson Cancer Center, Houston 77030, USA.
authors.forename: S
authors.initials: S
authors.lastname: Li
authors.forename: M F
authors.initials: MF
authors.lastname: Wilkinson
mesh_terms: D000818:Animals; D015252:Deoxyribonucleases, Type II Site-Specific / Q000378:metabolism*; D051379:Mice; D016297:Mutagenesis, Site-Directed; D016133:Polymerase Chain Reaction / Q000379:methods*; D016693:Receptors, Antigen, T-Cell, alpha-beta / Q000235:genetics
chemical_list: D016693:Receptors, Antigen, T-Cell, alpha-beta; C020102:endodeoxyribonuclease DpnI; D015252:Deoxyribonucleases, Type II Site-Specific