title: Complex subunit assembly of neuronal voltage-gated K+ channels. Basis for high-affinity toxin interactions and pharmacology.
abstract: Neurons require specific patterns of K+ channel subunit expression as well as the precise coassembly of channel subunits into heterotetrameric structures for proper integration and transmission of electrical signals. In vivo subunit coassembly was investigated by studying the pharmacological profile, distribution, and subunit composition of voltage-gated Shaker family K+ (Kv1) channels in rat cerebellum that are labeled by 125I-margatoxin (125I-MgTX; Kd, 0.08 pM). High-resolution receptor autoradiography showed spatial receptor expression mainly in basket cell terminals (52% of all cerebellar sites) and the molecular layer (39% of sites). Sequence-directed antibodies indicated overlapping expression of Kv1. 1 and Kv1.2 in basket cell terminals, whereas the molecular layer expressed Kv1.1, Kv1.2, Kv1.3, and Kv1.6 proteins. Immunoprecipitation experiments revealed that all 125I-MgTX receptors contain at least one Kv1.2 subunit and that 83% of these receptors are heterotetramers of Kv1.1 and Kv1.2 subunits. Moreover, 33% of these Kv1.1/Kv1.2-containing receptors possess either an additional Kv1.3 or Kv1.6 subunit. Only a minority of the 125I-MgTX receptors (<20%) seem to be homotetrameric Kv1.2 channels. Heterologous coexpression of Kv1.1 and Kv1.2 subunits in COS-1 cells leads to the formation of a complex that combines the pharmacological profile of both parent subunits, reconstituting the native MgTX receptor phenotype. Subunit assembly provides the structural basis for toxin binding pharmacology and can lead to the association of as many as three distinct channel subunits to form functional K+ channels in vivo.
authors.affiliation: Institute for Biochemical Pharmacology, Neuropharmacology Unit, University Innsbruck, Peter-Mayr Strasse 1, A-6020 Innsbruck, Austria.
authors.forename: R O
authors.initials: RO
authors.lastname: Koch
authors.forename: S G
authors.initials: SG
authors.lastname: Wanner
authors.forename: A
authors.initials: A
authors.lastname: Koschak
authors.forename: M
authors.initials: M
authors.lastname: Hanner
authors.forename: C
authors.initials: C
authors.lastname: Schwarzer
authors.forename: G J
authors.initials: GJ
authors.lastname: Kaczorowski
authors.forename: R S
authors.initials: RS
authors.lastname: Slaughter
authors.forename: M L
authors.initials: ML
authors.lastname: Garcia
authors.forename: H G
authors.initials: HG
authors.lastname: Knaus
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001345:Autoradiography; D002531:Cerebellum / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D007457:Iodine Radioisotopes; D015640:Ion Channel Gating; D008969:Molecular Sequence Data; D009474:Neurons / Q000187:drug effects / Q000378:metabolism*; D009498:Neurotoxins / Q000378:metabolism / Q000633:toxicity*; D015221:Potassium Channels / Q000187:drug effects / Q000378:metabolism*; D051381:Rats; D011994:Recombinant Proteins / Q000633:toxicity; D012604:Scorpion Venoms
chemical_list: D007457:Iodine Radioisotopes; D009498:Neurotoxins; D015221:Potassium Channels; D011994:Recombinant Proteins; D012604:Scorpion Venoms; C105268:margatoxin receptor; C077910:margatoxin