Gene expression of MASH-1, MATH-1, neuroD and NSCL-2, basic helix-loop-helix proteins, during neural differentiation in P19 embryonal carcinoma cells.
We examined the gene expression of MASH-1, MATH-1, neuroD and NSCL-2 during neural differentiation of P19 embryonal carcinoma cells using reverse transcription-polymerase chain reaction and high performance liquid chromatography. These proteins are members of basic helix-loop-helix transcription factor family and their expressions are reported to be transient and restricted in the nervous system during early neurogenesis. Retinoic acid (RA-, 1 microM)-treatment and aggregation for 4 days induced and greatly increased MASH-1, neuroD and NSCL-2 mRNA in P19 cells. The increases peaked at day 3, 4 and 5, respectively. RA-treatment increased MATH-1 mRNA slightly. mRNA of MAP2, a neural differentiation marker, were increased by RA-treatment and the increases reached to the plateau at day 5. The results indicate that the gene expression of MASH-1, MATH-1, neuroD and NSCL-2 during neural differentiation in P19 cells is transient and the order is similar to that in the mouse embryo nervous system as previously reported.
Itoh
F
F
Department of Pharmacology, Shinshu University School of Medicine, Matsumoto, Japan.
Nakane
T
T
Chiba
S
S
D000199:Actins / Q000096:biosynthesis; D000818:Animals; D051792:Basic Helix-Loop-Helix Proteins; D018236:Carcinoma, Embryonal / Q000235:genetics / Q000378:metabolism*; D002454:Cell Differentiation / Q000502:physiology; D002851:Chromatography, High Pressure Liquid; D004268:DNA-Binding Proteins / Q000096:biosynthesis; D015972:Gene Expression Regulation, Neoplastic / Q000235:genetics*; D018257:Helix-Loop-Helix Motifs / Q000235:genetics; D006801:Humans; D051379:Mice; D009419:Nerve Tissue Proteins / Q000096:biosynthesis; D009474:Neurons / Q000502:physiology*; D016133:Polymerase Chain Reaction; D012313:RNA / Q000096:biosynthesis; D014157:Transcription Factors / Q000096:biosynthesis
D000199:Actins; D051792:Basic Helix-Loop-Helix Proteins; D004268:DNA-Binding Proteins; D009419:Nerve Tissue Proteins; D012313:RNA; D014157:Transcription Factors; C065500:ASCL1 protein, human; C499317:Ascl1 protein, mouse; C500943:Atoh1 protein, mouse; C484104:Nhlh2 protein, mouse; C076747:NHLH2 protein, human; C093564:Neurogenic differentiation factor 1