Platelet-derived growth factor-BB, but not -AA, prevents delayed neuronal death after forebrain ischemia in rats.
Our previous studies demonstrated coordinate expression of platelet-derived growth factor (PDGF) -B chain and beta-receptor in neurons at risk in the rat brain with focal ischemia. To clarify a role of the -B chain in the brain further, we examined whether PDGF-A or -B chain protects CA1 pyramidal neurons from delayed neuronal death after forebrain ischemia in rats. Pretreatment with PDGF-BB, but not -AA, at 120 ng/d for 2 days until forebrain ischemia was performed markedly ameliorated delayed neuronal death in CA1 pyramidal neurons on day 7 after ischemia. This neuroprotective effect of PDGF-BB was dose-dependent, and pretreatment with PDGF-BB at 240 ng/d showed almost complete inhibition of delayed neuronal death. In contrast, posttreatment with PDGF-BB at 120 ng/d starting 20 minutes after ischemia demonstrated no significant neuroprotective effect. The current study established marked neuroprotective actions of PDGF-BB in ischemic neuronal damage.
Iihara
K
K
Department of Neurosurgery, Maizuru Municipal Hospital, Kyoto, Japan.
Hashimoto
N
N
Tsukahara
T
T
Sakata
M
M
Yanamoto
H
H
Taniguchi
T
T
D000818:Animals; D000925:Anticoagulants / Q000627:therapeutic use*; D000077214:Becaplermin; D016923:Cell Death; D005904:Glial Fibrillary Acidic Protein / Q000032:analysis; D006624:Hippocampus / Q000473:pathology; D002546:Ischemic Attack, Transient / Q000188:drug therapy* / Q000473:pathology*; D008297:Male; D009474:Neurons / Q000473:pathology*; D010982:Platelet-Derived Growth Factor / Q000627:therapeutic use*; D016548:Prosencephalon / Q000098:blood supply; D020574:Proto-Oncogene Proteins c-sis; D051381:Rats; D017208:Rats, Wistar
D000925:Anticoagulants; D005904:Glial Fibrillary Acidic Protein; D010982:Platelet-Derived Growth Factor; D020574:Proto-Oncogene Proteins c-sis; C075278:platelet-derived growth factor A; D000077214:Becaplermin