Temperature-induced expression of human-mouse chimeric Fab.
The temperature-induced expression vector pHZ01 with lambda PRPL promoter for the efficient expression of human-mouse chimeric Fab was constructed. Three kinds of chimeric Fab were expressed in E. coli: anti-prostate specific antigen (PSA) chimeric Fab, anti-lysozyme (HEL) chimeric Fab, and anti-tetanus toxoid (TT) chimeric Fab. All the soluble chimeric Fabs expressed showed specific antigen-binding activities.
Zan
H
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Shanghai Institute of Cell Biology, Chinese Academy of Sciences, China.
Yeh
M
M
D000818:Animals; D000937:Antigen-Antibody Reactions; D000941:Antigens / Q000235:genetics / Q000276:immunology; D004926:Escherichia coli / Q000378:metabolism; D015870:Gene Expression; D005822:Genetic Vectors; D006361:Heating; D006801:Humans; D007140:Immunoglobulin Fab Fragments / Q000096:biosynthesis* / Q000235:genetics / Q000276:immunology; D051379:Mice; D009113:Muramidase / Q000235:genetics / Q000276:immunology; D017430:Prostate-Specific Antigen / Q000235:genetics / Q000276:immunology; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis / Q000235:genetics / Q000276:immunology; D013745:Tetanus Toxoid / Q000235:genetics / Q000276:immunology
D000941:Antigens; D007140:Immunoglobulin Fab Fragments; D011993:Recombinant Fusion Proteins; D013745:Tetanus Toxoid; D009113:Muramidase; D017430:Prostate-Specific Antigen