Metal ion chaperone function of the soluble Cu(I) receptor Atx1.
Reactive and potentially toxic cofactors such as copper ions are imported into eukaryotic cells and incorporated into target proteins by unknown mechanisms. Atx1, a prototypical copper chaperone protein from yeast, has now been shown to act as a soluble cytoplasmic copper(I) receptor that can adopt either a two- or three-coordinate metal center in the active site. Atx1 also associated directly with the Atx1-like cytosolic domains of Ccc2, a vesicular protein defined in genetic studies as a member of the copper-trafficking pathway. The unusual structure and dynamics of Atx1 suggest a copper exchange function for this protein and related domains in the Menkes and Wilson disease proteins.
Pufahl
R A
RA
Department of Chemistry, Northwestern University, Evanston, IL 60208, USA.
Singer
C P
CP
Peariso
K L
KL
Lin
S J
SJ
Schmidt
P J
PJ
Fahrni
C J
CJ
Culotta
V C
VC
Penner-Hahn
J E
JE
O'Halloran
T V
TV
D000595:Amino Acid Sequence; D002352:Carrier Proteins; D027682:Cation Transport Proteins; D003300:Copper / Q000378:metabolism*; D000079922:Copper Transport Proteins; D004926:Escherichia coli; D005656:Fungal Proteins / Q000378:metabolism / Q000502:physiology*; D006801:Humans; D018832:Molecular Chaperones / Q000502:physiology*; D008969:Molecular Sequence Data; D011994:Recombinant Proteins; D012441:Saccharomyces cerevisiae / Q000378:metabolism / Q000502:physiology*; D029701:Saccharomyces cerevisiae Proteins; D017386:Sequence Homology, Amino Acid
C093341:ATX1 protein, S cerevisiae; C092974:CCC2 protein, S cerevisiae; D002352:Carrier Proteins; D027682:Cation Transport Proteins; D000079922:Copper Transport Proteins; D005656:Fungal Proteins; D018832:Molecular Chaperones; D011994:Recombinant Proteins; D029701:Saccharomyces cerevisiae Proteins; D003300:Copper