title: Response and recovery of acethylcholinesterase activity in the European eel Anguilla anguilla exposed to fenitrothion.
abstract: European eel (Anguilla anguilla) were exposed to sublethal fenitrothion concentrations in a continuous flow-through system for 4 days. Muscle acetylcholinesterase (AChE) activity was evaluated after 2, 8, 12, 24, 32, 48, 56, 72 and 96 hours pesticide exposure. Results showed that AChE activity in eel muscle tissue decreased as concentration of fenitrothion increased. Pesticide induced significant inhibitory effects on the AChE activity of A. anguilla ranging from > 35% inhibition at sublethal concentration of 0.02 ppm to > 44% inhibition at sublethal concentration of 0.04 ppm. Eel were exposed to both fenitrothion concentrations for 96 hours and then allowed a period of recovery in pesticide-free water. Samples were taken out at 8, 12, 24, 48, 72, 96, 144 and 192 hours and eel muscle AChE activity was evaluated. Following 1 week of recovery, the AChE activity for those animals previously exposed to 0.02 and 0.04 ppm fenitrothion was still different from the controls. So, the AChE activity of eel muscle at the end of the recovery phases remained significantly depressed.
authors.affiliation: Dept. Animal Physiology, Faculty of Biological Sciences, Burjasot, Valencia, Spain.
authors.forename: E
authors.initials: E
authors.lastname: Sancho
authors.forename: M D
authors.initials: MD
authors.lastname: Ferrando
authors.forename: E
authors.initials: E
authors.lastname: Andreu
mesh_terms: D000110:Acetylcholinesterase / Q000032:analysis* / Q000378:metabolism; D000811:Anguilla; D000818:Animals; D002800:Cholinesterase Inhibitors / Q000633:toxicity*; D002849:Chromatography, Gas; D004305:Dose-Response Relationship, Drug; D005278:Fenitrothion / Q000008:administration & dosage / Q000633:toxicity*; D007306:Insecticides / Q000633:toxicity*; D007928:Lethal Dose 50; D009132:Muscles / Q000187:drug effects / Q000201:enzymology*
chemical_list: D002800:Cholinesterase Inhibitors; D007306:Insecticides; D000110:Acetylcholinesterase; D005278:Fenitrothion