In vitro binding of purified murine ecotropic retrovirus envelope surface protein to its receptor, MCAT-1.
An amino-terminal portion of the Friend murine leukemia virus (MLV) envelope surface protein [SU, residues 1 to 236 [SU:(1-236)]] and its receptor, MCAT-1, were each purified from insect cells after expression by using recombinant baculoviruses. Friend SU:(1-236) bound specifically to Xenopus oocytes that expressed MCAT-1 with an affinity (Kd, 55 nM) similar to that of viral SU binding to permissive cells. Direct binding of Friend SU:(1-236) to purified MCAT-1 was observed in detergent and after reconstitution into liposomes. Analysis of binding demonstrated that MCAT-1 and Friend SU:(1-236) interact with a stoichiometry of near 1:1. These findings demonstrate that the amino-terminal domain from the SU of ecotropic murine retroviruses contains an MCAT-1 binding domain.
Davey
R A
RA
Howard Hughes Medical Institute, Department of Medicine, Brigham and Women's Hospital and Harvard Medical School, Boston, Massachusetts 02115, USA.
Hamson
C A
CA
Healey
J J
JJ
Cunningham
J M
JM
D000818:Animals; D016367:Baculoviridae / Q000235:genetics; D001665:Binding Sites; D002352:Carrier Proteins / Q000737:chemistry / Q000378:metabolism*; D006224:Cricetinae; D005622:Friend murine leukemia virus / Q000737:chemistry / Q000378:metabolism*; D008081:Liposomes; D008562:Membrane Glycoproteins; D008565:Membrane Proteins / Q000737:chemistry / Q000378:metabolism*; D051379:Mice; D008969:Molecular Sequence Data; D011485:Protein Binding; D011991:Receptors, Virus / Q000378:metabolism*; D011994:Recombinant Proteins; D014759:Viral Envelope Proteins / Q000378:metabolism*
D002352:Carrier Proteins; D008081:Liposomes; D008562:Membrane Glycoproteins; D008565:Membrane Proteins; D011991:Receptors, Virus; D011994:Recombinant Proteins; D014759:Viral Envelope Proteins; C070506:ecotropic murine leukemia virus receptor