The pharmacological profile of mouse hind paw inflammation induced by staphylococcal enterotoxin type A.
OBJECTIVE
The present paper examines the possible pharmacological mediators involved in mouse hind paw inflammation induced by staphylococcal enterotoxin type A (SEA).


METHODS
The edema and the Evans blue exudation were measured in male Swiss mice (20-25 g) using methods described by Levy and Griswold, respectively.


RESULTS
SEA (32 micrograms/paw) produced a biphasic, long-lasting, dose- and time-dependent edematogenic response. The acute phase edema was pronounced while the chronic edema was of a low intensity. Exudate was the principal component of the edema. The edematogenic effect of SEA appears to involve cyclooxygenase products and was dose-dependently reduced by pretreating the mice with dexamethasone, indomethacin, BW755C, WEB2086, capsaicin, diphenhydramine or cimetidine.


CONCLUSIONS
These results demonstrate that SEA-induced mouse hind paw inflammation is a useful model for studying SEA-mediated enterotoxemia and may be sufficiently sensitive to differentiate between the effects of SEA and those of SE type B (SEB).
Desouza
I A
IA
Department of Pharmacology, Faculty of Medical Sciences, State University of Campinas, Cidade Universitária, Zeferino Vaz, Brazil.
Ribeiro-DaSilva
G
G
D015772:4,5-Dihydro-1-(3-(trifluoromethyl)phenyl)-1H-pyrazol-3-amine / Q000627:therapeutic use; D000818:Animals; D001381:Azepines / Q000627:therapeutic use; D002211:Capsaicin / Q000627:therapeutic use; D002927:Cimetidine / Q000627:therapeutic use; D003907:Dexamethasone / Q000627:therapeutic use; D004155:Diphenhydramine / Q000627:therapeutic use; D004487:Edema / Q000139:chemically induced / Q000188:drug therapy; D004768:Enterotoxins; D004791:Enzyme Inhibitors / Q000494:pharmacology; D006614:Hindlimb; D006633:Histamine Antagonists / Q000494:pharmacology; D007213:Indomethacin / Q000627:therapeutic use; D007249:Inflammation / Q000139:chemically induced* / Q000188:drug therapy*; D008297:Male; D051379:Mice; D010975:Platelet Aggregation Inhibitors / Q000494:pharmacology; D014230:Triazoles / Q000627:therapeutic use
D001381:Azepines; D004768:Enterotoxins; D004791:Enzyme Inhibitors; D006633:Histamine Antagonists; D010975:Platelet Aggregation Inhibitors; D014230:Triazoles; C052518:WEB 2086; C026066:enterotoxin A, Staphylococcal; D015772:4,5-Dihydro-1-(3-(trifluoromethyl)phenyl)-1H-pyrazol-3-amine; D003907:Dexamethasone; D002927:Cimetidine; D004155:Diphenhydramine; D002211:Capsaicin; D007213:Indomethacin