title: Effect of unsaturated fatty acids and alpha-tocopherol on immunoglobulin levels in culture medium of rat mesenteric lymph node and spleen lymphocytes.
abstract: Mesenteric lymph node (MLN) and spleen lymphocytes of Sprague-Dawley rats were cultured with 1 mM unsaturated fatty acids (UFAs) with or without 100 microM alpha-tocopherol (Toc), and the immunoglobulin content and thiobarbituric acid (TBA) value of the culture media were measured to clarify the relationship between lipid peroxidation and the IgE level in the culture medium. The increase in the IgE content and TBA value induced by UFAs was alleviated in the presence of Toc in both lymphocytes, and was correlated well with their oxidation rates in most cases. Gamma-linolenic acid enhanced the IgE level much more than would be expected from its oxidation rate in both lymphocytes, and linoleic acid showed similarly high activity only in splenocytes. These results suggest that lipid peroxidation is partly responsible for the enhancement of IgE level induced by UFAs.
authors.affiliation: Department of Food Science and Technology, Faculty of Agriculture, Kyushu University, Fukuoka.
authors.forename: P
authors.initials: P
authors.lastname: Hùng
authors.forename: K
authors.initials: K
authors.lastname: Yamada
authors.forename: B O
authors.initials: BO
authors.lastname: Lim
authors.forename: M
authors.initials: M
authors.lastname: Mori
authors.forename: T
authors.initials: T
authors.lastname: Yuki
authors.forename: M
authors.initials: M
authors.lastname: Sugano
mesh_terms: D000818:Animals; D002470:Cell Survival / Q000187:drug effects; D003470:Culture Media; D005231:Fatty Acids, Unsaturated / Q000494:pharmacology*; D007073:Immunoglobulin E / Q000378:metabolism; D007136:Immunoglobulins / Q000378:metabolism*; D008054:Lipid Peroxides / Q000378:metabolism; D008198:Lymph Nodes / Q000187:drug effects* / Q000276:immunology; D008214:Lymphocytes / Q000187:drug effects* / Q000276:immunology; D008643:Mesentery; D051381:Rats; D017207:Rats, Sprague-Dawley; D013154:Spleen / Q000187:drug effects* / Q000276:immunology; D017392:Thiobarbituric Acid Reactive Substances / Q000378:metabolism; D014810:Vitamin E / Q000494:pharmacology*
chemical_list: D003470:Culture Media; D005231:Fatty Acids, Unsaturated; D007136:Immunoglobulins; D008054:Lipid Peroxides; D017392:Thiobarbituric Acid Reactive Substances; D014810:Vitamin E; D007073:Immunoglobulin E