title: Induction of a particular deletion in mitochondrial DNA by X rays depends on the inherent radiosensitivity of the cells.
abstract: We examined whether X radiation induces a particular deletion in the mitochondrial DNA (mtDNA) of the cells of two human squamous cell carcinoma lines with different sensitivity to radiation and in a radiosensitive ataxia telangiectasia (AT) cell line. We used polymerase chain reaction (PCR) to quantify the accumulation of a particular 4977-bp deletion (delta mtDNA4977). PCR products of delta mtDNA4977 were detectable after exposure to 10 Gy in the radioresistant squamous cell carcinoma cells, 2 Gy in the radiosensitive squamous cell carcinoma cells and 1 Gy in the radiosensitive AT cells. These observations suggest that ionizing radiation induces the delta mtDNA4977 in human cells and that the radiation doses required to induce this deletion reflect the sensitivity of cells to radiation.
authors.affiliation: Department of Radiological Sciences, Ibaraki Prefectural University of Health Sciences, Japan.
authors.forename: N
authors.initials: N
authors.lastname: Kubota
authors.forename: J
authors.initials: J
authors.lastname: Hayashi
authors.forename: T
authors.initials: T
authors.lastname: Inada
authors.forename: Y
authors.initials: Y
authors.lastname: Iwamura
mesh_terms: D002294:Carcinoma, Squamous Cell / Q000235:genetics / Q000473:pathology; D004249:DNA Damage; D004272:DNA, Mitochondrial / Q000528:radiation effects*; D004273:DNA, Neoplasm / Q000528:radiation effects*; D006258:Head and Neck Neoplasms / Q000235:genetics / Q000473:pathology; D006801:Humans; D016133:Polymerase Chain Reaction; D011836:Radiation Tolerance; D017384:Sequence Deletion; D014407:Tumor Cells, Cultured / Q000528:radiation effects; D014965:X-Rays
chemical_list: D004272:DNA, Mitochondrial; D004273:DNA, Neoplasm