Different expression of adenylyl cyclase isoforms after retinoic acid induction of P19 teratocarcinoma cells.
We have investigated the adenylyl cyclase (AC) activity and gene expression in retinoic acid (RA)-primed murine P19 teratocarcinoma cells, which recapitulate in vitro the first stages of neuroectodermal formation. Here we show that the P19 stem cells possess a basal Ca2+/CaM-stimulated AC activity, which increases about 10-fold after RA induction. The rise of AC activity is associated with a stage-specific up-regulation of AC2, AC5 and AC8 mRNAs and a down-regulation of AC3 mRNA. P19 cells provide a powerful model to investigate the role and specific regulation of AC isoforms during neuronal differentiation.
Lipskaia
L
L
Institut National de la Santé et de la Recherche Médicale U-99, Hôpital Henri Mondor, Créteil, France.
Djiane
A
A
Defer
N
N
Hanoune
J
J
D000262:Adenylyl Cyclases / Q000235:genetics* / Q000378:metabolism; D000818:Animals; D015152:Blotting, Northern; D002118:Calcium / Q000494:pharmacology; D002147:Calmodulin / Q000494:pharmacology; D002454:Cell Differentiation; D015342:DNA Probes; D015536:Down-Regulation / Q000502:physiology; D015971:Gene Expression Regulation, Enzymologic; D007527:Isoenzymes / Q000235:genetics / Q000378:metabolism; D051379:Mice; D009474:Neurons / Q000166:cytology / Q000201:enzymology*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D013234:Stem Cells / Q000166:cytology / Q000201:enzymology*; D018243:Teratocarcinoma; D014212:Tretinoin / Q000494:pharmacology*; D014407:Tumor Cells, Cultured; D015854:Up-Regulation / Q000502:physiology
D002147:Calmodulin; D015342:DNA Probes; D007527:Isoenzymes; D012333:RNA, Messenger; D014212:Tretinoin; D000262:Adenylyl Cyclases; D002118:Calcium