Purification and characterization of phenylacetaldehyde reductase from a styrene-assimilating Corynebacterium strain, ST-10.
A novel phenylacetaldehyde reductase was purified about 50-fold to homogeneity from Corynebacterium sp. strain ST-10, which can assimilate gaseous styrene as the sole carbon and energy source. The enzyme was inductively synthesized when grown on gaseous styrene and had an important role in styrene metabolism in vivo. The enzyme had a molecular weight of 155,000 and was composed of four identical subunits (molecular weight, 42,000). The enzyme catalyzed the reduction of not only phenylacetaldehyde but also various aldehydes and ketones; however, it did not catalyze the reverse reaction, the dehydrogenation of 2-phenylethanol. The enzyme required NADH as a cofactor and showed no activity with NADPH; therefore, it was defined as an NADH-dependent phenylacetaldehyde reductase. The enzyme stereospecifically produced (S)-(-)-1-phenylethanol from acetophenone; therefore, it would be useful as a biocatalyst.
Itoh
N
N
Department of Applied Chemistry and Biotechnology, Faculty of Engineering, Fukui University, Japan.
Morihama
R
R
Wang
J
J
Okada
K
K
Mizuguchi
N
N
D000079:Acetaldehyde / Q000031:analogs & derivatives / Q000378:metabolism; D000426:Alcohol Dehydrogenase / Q000235:genetics; D000429:Alcohol Oxidoreductases / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism; D000447:Aldehydes; D000595:Amino Acid Sequence; D000818:Animals; D001673:Biodegradation, Environmental; D003352:Corynebacterium / Q000235:genetics / Q000378:metabolism*; D006801:Humans; D007659:Ketones; D007700:Kinetics; D007064:L-Iditol 2-Dehydrogenase / Q000235:genetics; D008969:Molecular Sequence Data; D008970:Molecular Weight; D009243:NAD / Q000378:metabolism; D011487:Protein Conformation; D017386:Sequence Homology, Amino Acid; D013237:Stereoisomerism; D020058:Styrene; D013343:Styrenes / Q000378:metabolism*; D013379:Substrate Specificity
D000447:Aldehydes; D007659:Ketones; D013343:Styrenes; D009243:NAD; D020058:Styrene; D000429:Alcohol Oxidoreductases; C110191:phenylacetaldehyde reductase; D000426:Alcohol Dehydrogenase; D007064:L-Iditol 2-Dehydrogenase; D000079:Acetaldehyde; C013192:phenylacetaldehyde