title: Biochemical alterations of the tunica albuginea in impotence.
abstract: OBJECTIVE
  The objectives of this study were to quantify the immunohistochemical stainings of collagen types I, III and IV, and investigate the value of glycohistochemical staining with 3 lectin types specific to a particular glycan structure, Arachis hypogaea, Triticum vulgare and concanavalin A, as a method of defining possible changes in the collagen structure of the tunica albuginea in potent and impotent patients.
  
  
  METHODS
  The study involved 4 normal men, 4 with pure venous leakage and 4 with pure arterial disease. Collagen types I, III and IV, and lectins Arachis hypogaea, Triticum vulgare and concanavalin A were studied using a cell image processor. The labeling index relates to the percentage of staining and mean optical density relates to the staining intensity.
  
  
  RESULTS
  Mean labeling index values for the 3 types of collagen and lectins were similar (p > 0.05). Mean optical density value relating to collagen type I was significantly higher in the arteriogenic group than in the other groups (p < 0.05), while mean optical density value of collagen type IV was significantly higher in the venogenic group than in the 2 other groups (p < 0.05). Mean optical density values relating to the 3 lectin types were similar in the 3 clinical groups (p > 0.05).
  
  
  CONCLUSIONS
  An alteration in the distribution and structure of the various collagen types and lectins in the tunica albuginea of impotent patients has been shown that may interfere with normal function and lead to impotence.
authors.affiliation: Department of Urology, Erasme Hospital, Brussels, Belgium.
authors.forename: G
authors.initials: G
authors.lastname: Raviv
authors.forename: J P
authors.initials: JP
authors.lastname: Vanegas
authors.forename: M
authors.initials: M
authors.lastname: Petein
authors.forename: C
authors.initials: C
authors.lastname: Schulman
authors.forename: A
authors.initials: A
authors.lastname: Danguy
authors.forename: R
authors.initials: R
authors.lastname: Kiss
authors.forename: E
authors.initials: E
authors.lastname: Wespes
mesh_terms: D000328:Adult; D003094:Collagen / Q000032:analysis / Q000096:biosynthesis*; D006801:Humans; D018783:Impotence, Vasculogenic / Q000378:metabolism*; D037102:Lectins; D008297:Male; D008875:Middle Aged; D010413:Penis / Q000737:chemistry / Q000378:metabolism*
chemical_list: D037102:Lectins; D003094:Collagen