Molecular cloning of a novel alternatively spliced nuclear protein.
Using the yeast two hybrid system, we isolated a rat cDNA (E3-3) coding for a new protein with no homology to any other protein in the database. E3-3 is ubiquitously expressed. Variants that most likely arise through alternative splicing encode truncated forms of the protein. Testis is the only tissue that predominantly expresses the longest protein variant. When this variant is tagged with enhanced green fluorescent protein, the protein is located in the nucleus.
Hartmann
A M
AM
Max-Planck Institute for Psychiatry, Planegg Martinsried, Germany.
Stamm
S
S
D017398:Alternative Splicing; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002467:Cell Nucleus / Q000737:chemistry; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000032:analysis / Q000235:genetics*; D008297:Male; D008969:Molecular Sequence Data; D009687:Nuclear Proteins / Q000032:analysis / Q000235:genetics*; D016366:Open Reading Frames / Q000235:genetics; D009928:Organ Specificity; D051381:Rats; D011993:Recombinant Fusion Proteins / Q000032:analysis; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid
D018076:DNA, Complementary; C108804:E3-3 protein, rat; D009687:Nuclear Proteins; D011993:Recombinant Fusion Proteins