Antibody-mediated detection and physical properties of oligonucleotides labelled with multiple internal and terminal 2,4-dinitrophenyl groups.
DNP-labelled phosphoramidites have been used to synthesis oligonucleotides with multiple DNP reporter groups. The antibody-mediated detection and the stability of duplexes formed by these labelled oligonucleotides have been studied. A DNP-labelled deoxyuridine triphosphate has also been used to enzymatically incorporate DNP-labels into DNA via the polymerase chain reaction. The use of DNP-labelled primers in the PCR has also been investigated.
McClean
J
J
Department of Chemistry, University of Southampton, Highfield, England, U.K.
Davison
A
A
Rao
M V
MV
Brown
T
T
D000906:Antibodies / Q000276:immunology*; D017931:DNA Primers / Q000378:metabolism; D004136:Dinitrobenzenes / Q000737:chemistry*; D009693:Nucleic Acid Hybridization; D009841:Oligonucleotides / Q000138:chemical synthesis / Q000737:chemistry* / Q000276:immunology; D016133:Polymerase Chain Reaction / Q000379:methods; D014466:Ultraviolet Rays
D000906:Antibodies; D017931:DNA Primers; D004136:Dinitrobenzenes; D009841:Oligonucleotides