title: Epithelial galectin-3 during human nephrogenesis and childhood cystic diseases.
abstract: Galectin-3 is a beta-galactoside-binding protein with putative roles in development, oncogenesis, and inflammation. Its expression in human nephrogenesis has not been previously reported. This study examines galectin-3 expression in early human embryos by Western blot and immunohistochemistry. This 33-kD protein was detected in the apical domain of distal tubules of the mesonephros and also in the mesonephric duct. In the metanephros, the adult kidney precursor, galectin-3 was detected in the apical domains of ureteric bud branches, and there was intense expression in fetal medullary and papillary collecting ducts in both the cytoplasm and plasma membranes. Low levels of galectin-3 were detected in the cytoplasm of a subset of cells in adult collecting ducts; these were alpha-intercalated cells because they expressed basal band 3 protein. In human multicystic dysplastic kidneys, all diseased epithelia had an embryonic apical expression pattern of galectin-3 and, in addition, all cystic epithelia in autosomal recessive polycystic kidneys expressed this molecule. It is concluded that galectin-3 is expressed by cells of the mesonephric duct/ureteric bud lineage, and it is speculated that the different subcellular locations may be implicated in both the regulation of normal growth and differentiation of this lineage, as well as in the pathogenesis of cystic epithelia.
authors.affiliation: Developmental Biology Unit, Institute of Child Health, London, United Kingdom.
authors.forename: P J
authors.initials: PJ
authors.lastname: Winyard
authors.forename: Q
authors.initials: Q
authors.lastname: Bao
authors.forename: R C
authors.initials: RC
authors.lastname: Hughes
authors.forename: A S
authors.initials: AS
authors.lastname: Woolf
mesh_terms: D000328:Adult; D000943:Antigens, Differentiation / Q000032:analysis*; D015153:Blotting, Western; D046508:Culture Techniques; D005314:Embryonic and Fetal Development; D004848:Epithelium / Q000737:chemistry / Q000473:pathology; D005260:Female; D037502:Galectin 3; D006801:Humans; D007150:Immunohistochemistry; D007668:Kidney / Q000196:embryology* / Q000473:pathology; D008562:Membrane Glycoproteins / Q000032:analysis*; D007690:Polycystic Kidney Diseases / Q000196:embryology* / Q000473:pathology; D011247:Pregnancy; D012680:Sensitivity and Specificity; D014551:Urinary Tract / Q000196:embryology / Q000473:pathology; D019459:Urothelium / Q000737:chemistry / Q000473:pathology
chemical_list: D000943:Antigens, Differentiation; D037502:Galectin 3; D008562:Membrane Glycoproteins