Synthetic genes specifying periodic polymers modelled on the repetitive domain of wheat gliadins: conception and expression.
In order to optimise new polypeptide based biomaterials, we developed a procedure for producing homoblock polypeptides using recombinant DNA technology. Synthetic genes encoding periodic polypeptides modelled on the consensus sequence of wheat gliadins (a family of wheat storage proteins) were devised to be expressed in Escherichia coli. The construction strategy followed allows the construction of three genes encoding 8, 16, and 32 copies of the PQQPY module. The optimal expression conditions in the enterobacteria were established and a convenient purification procedure was shown to be useful in recovery of sizable amounts of strictly periodic polypeptides. The identities of the synthesized polypeptides were assessed using positive cross reactions to antibodies raised against a synthetic decapeptide (PQQPYPQQPA) and amino acid composition was determined as well.
Elmorjani
K
K
Laboratoire de Biochimie et de Technologie des Protéines, INRA, Nantes, France. elmorjan@nat.svt.sciences.univ-nantes.fr
Thiévin
M
M
Michon
T
T
Popineau
Y
Y
Hallet
J N
JN
Guéguen
J
J
D000595:Amino Acid Sequence; D001483:Base Sequence; D016384:Consensus Sequence; D004926:Escherichia coli; D005813:Genes, Synthetic; D005903:Gliadin / Q000235:genetics*; D008969:Molecular Sequence Data; D011108:Polymers; D011994:Recombinant Proteins / Q000737:chemistry; D012091:Repetitive Sequences, Nucleic Acid; D015183:Restriction Mapping; D014908:Triticum / Q000235:genetics*
D011108:Polymers; D011994:Recombinant Proteins; D005903:Gliadin