Single-strand breaks and repair in nuclear DNA in the presence of hydralazine assayed by the nucleoid technique.
The nucleoid sedimentation assay was used to study hydralazine-induced DNA structural changes and repair in the fibroblasts cultured in vitro. The drug induced a dose dependent loss in negative DNA supercoiling due to the physical breakage of the DNA. Relaxation of supercoiled DNA resulted in the nucleoids sedimenting with lower velocities than those of undamaged control cultures. Repair incubation of the cells did not cause the restoration of DNA supercoiling to control level. Unsuccessful repair of DNA damaged by hydralazine may result in maintaining the damaged DNA in the cell which could have immunologic consequences.
Weglarz
L
L
Department of Biochemistry and Chemistry, Silesian Medical Academy, Katowice, Poland.
Koceva-Chyła
A
A
Drózdz
M
M
Jóźwiak
Z
Z
D016475:3T3 Cells / Q000187:drug effects; D000818:Animals; D004249:DNA Damage / Q000187:drug effects* / Q000235:genetics; D004260:DNA Repair / Q000235:genetics; D004277:DNA, Single-Stranded / Q000187:drug effects / Q000235:genetics*; D004278:DNA, Superhelical / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D006830:Hydralazine / Q000494:pharmacology*; D051379:Mice; D008807:Mice, Inbred BALB C; D009690:Nucleic Acid Conformation / Q000187:drug effects*; D014665:Vasodilator Agents / Q000494:pharmacology*
D004277:DNA, Single-Stranded; D004278:DNA, Superhelical; D014665:Vasodilator Agents; D006830:Hydralazine