Accumulation of insulin-gold particles in the oral apparatus of Tetrahymena after insulin pretreatment (imprinting).
The oral apparatus and body ciliature of untreated control cells bind insulin-gold very rarely. From 1 day to 1 week after insulin pretreatment (hormonal imprinting) an enormous quantity of insulin-gold was bound by the oral cilia, completely filling the region, while the insulin-gold on the body ciliature is scattered. The binding was specific for insulin, since polyethylene glycol (PEG)-gold was not bound at all. The results call attention to the binding and the increasing role of hormonal (insulin) imprinting, and particularly to the marked role of the oral region in this binding. The roles of mucocyst extrusion and specific binding by receptors are discussed.
Fülöp
A K
AK
Department of Genetics, Cell and Immunobiology, Semmelweis University of Medicine, Budapest, Hungary.
Csaba
G
G
D000818:Animals; D002923:Cilia / Q000378:metabolism; D006046:Gold; D006651:Histocytochemistry / Q000379:methods*; D007328:Insulin / Q000378:metabolism*; D008854:Microscopy, Electron; D008855:Microscopy, Electron, Scanning; D015388:Organelles / Q000378:metabolism*; D011092:Polyethylene Glycols / Q000378:metabolism; D011485:Protein Binding; D012680:Sensitivity and Specificity; D013768:Tetrahymena / Q000166:cytology / Q000378:metabolism* / Q000648:ultrastructure
D007328:Insulin; D011092:Polyethylene Glycols; D006046:Gold