Genetic analysis of regions involved in replication and cadmium resistance of the plasmid pND302 from Lactococcus lactis.
The 8.8-kb Lactococcus lactis plasmid pND302 encodes resistance to cadmium (CdR). Regions of pND302 involved in replication and CdR were subcloned and sequenced. The replication region is localized on a 1.5-kb region and consists of an open reading frame (repB) preceded by a noncoding AT-rich sequence (ori) which is highly homologous to lactococcal theta-type replicons. The CdR determinant is localized on a 2.9-kb region and encodes putative proteins similar to the Cd(2+)-specific P-type efflux ATPase (CadA) and the transcriptional regulatory repressor (CadC) identified in Staphylococcus aureus, Bacillus firmus, and Listeria monocytogenes. Similar CdR determinants were also detected by PCR in other CdR plasmids isolated from different L. lactis strains.
Liu
C Q
CQ
Department of Biotechnology, University of New South Wales, Sydney, Australia.
Khunajakr
N
N
Chia
L G
LG
Deng
Y M
YM
Charoenchai
P
P
Dunn
N W
NW
D000251:Adenosine Triphosphatases / Q000235:genetics; D002104:Cadmium / Q000494:pharmacology*; D004261:DNA Replication; D004352:Drug Resistance, Microbial / Q000235:genetics*; D013294:Lactococcus lactis / Q000235:genetics*; D010957:Plasmids / Q000235:genetics*; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid
D002104:Cadmium; D000251:Adenosine Triphosphatases