title: Measurement of renal vein blood flow in rats by high-field magnetic resonance.
abstract: The aim of the present study was to examine whether magnetic resonance imaging (MRI) based method for non-invasive in vivo measurement of vein blood flow in rats could be used to estimate renal blood flow (RBF). Measurements were performed using a high-field (7 Tesla) MRI scanner with a short echo time phase contrast velocity measurement pulse sequence. The method was evaluated in vitro by flow measurements in an acrylic pipe and in vivo by recording left renal vein blood flow in normal and unilaterally nephrectomized rats. In a subset of animals RBF was measured by a direct method using 14C-tetraethylammoniumbromide. In vitro a high accuracy was found between applied and MRI measured flow rates in the range from 0.5 to 33 ml/min (r = 0.997; P < 0.001). In vivo the MRI measured left renal vein blood flow was 70% higher in unilaterally nephrectomized animals compared to control animals (3.4 +/- 0.4 ml/min/ 100 g body wt vs. 2.0 +/- 0.1 ml/min/100 g body wt, P < 0.001). Direct measurements of RBF revealed comparable values (3.4 +/- 0.3 ml/min/100 g body wt vs. 2.3 +/- 0.4 ml/min/100 g body wt, P = 0.05). In addition, the left kidney volume was recorded by MRI with an increase amounting to 40% (1.18 +/- 0.05 ml vs. 0.84 +/- 0.02 ml; P < 0.001) in the nephrectomized group compared to controls. Finally, a positive correlation was seen between left renal vein blood flow and MRI measured renal volume (r = 0.91; P < 0.001). In summary, MRI is a non-invasive tool by which measurement of renal vein blood flow can be performed, and it is concluded that MRI-based renal vein flow measurements can be used to estimate RBF in small rodents.
authors.affiliation: Magnetic Resonance Research Centre, Aarhus University Hospital, Aarhus University, Denmark. skejsri@aau.dk
authors.forename: S
authors.initials: S
authors.lastname: Ringgaard
authors.forename: T
authors.initials: T
authors.lastname: Christiansen
authors.forename: M
authors.initials: M
authors.lastname: Bak
authors.forename: E M
authors.initials: EM
authors.lastname: Pedersen
authors.forename: H
authors.initials: H
authors.lastname: Stødkilde-Jørgensen
authors.forename: A
authors.initials: A
authors.lastname: Flyvbjerg
mesh_terms: D000818:Animals; D005260:Female; D008279:Magnetic Resonance Imaging; D051381:Rats; D017208:Rats, Wistar; D012079:Renal Circulation; D012082:Renal Veins / Q000502:physiology*