Multiplex systems for the amplification of short tandem repeat loci: evaluation of laser fluorescence detection.
Short tandem repeat (STR) loci are ideal markers for personal identification and for genomic mapping. Two fluorescent multiplex systems, each designed for simultaneous PCR amplification of four polymorphic STR loci (HUMCSF1PO, HUMTPOX, HUMTH01 and HUMVWFA31, and HUMF13A01, HUMFESFPS, HUMBFXIII and HUMLIPOL), were evaluated on three laser fluorescence detection instruments. Concordant DNA typing results were obtained with all three detection methods. These fluorescent multiplex STR systems offer an accurate, reproducible and versatile method of DNA profiling that is well-suited for forensic identity testing and other genetic analyses.
Ricciardone
M D
MD
Armed Forces Institute of Pathology, Rockville, MD, USA. ricciard@fen.bilkent.edu.tr
Lins
A M
AM
Schumm
J W
JW
Holland
M M
MM
D000483:Alleles; D004247:DNA / Q000032:analysis*; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods; D005456:Fluorescent Dyes; D005470:Fluorometry / Q000379:methods*; D007834:Lasers; D016133:Polymerase Chain Reaction; D011786:Quality Control; D012091:Repetitive Sequences, Nucleic Acid; D012680:Sensitivity and Specificity; D017422:Sequence Analysis, DNA / Q000295:instrumentation / Q000379:methods*
D005456:Fluorescent Dyes; D004247:DNA