Emerging tandem-mass-spectrometry techniques for the rapid identification of proteins.
State-of-the-art techniques such as liquid-chromatography-electrospray-ionisation tandem mass spectrometry have, in conjunction with database-searching computer algorithms, revolutionised the analysis of biochemical species from complex biological mixtures. With these techniques, it is now possible to perform high-throughput protein identification at picomolar to subpicomolar levels from protein mixtures. This article provides an overview of the techniques and methodologies available for the structural elucidation and identification of proteins and peptides from complex biological samples.
Dongré
A R
AR
Department of Molecular Biotechnology, University of Washington, Seattle 98195, USA. adongre@u.washington.edu
Eng
J K
JK
Yates
J R
JR
D000465:Algorithms; D000595:Amino Acid Sequence; D001709:Biotechnology; D002851:Chromatography, High Pressure Liquid / Q000295:instrumentation / Q000379:methods / Q000706:statistics & numerical data; D003627:Data Interpretation, Statistical; D013058:Mass Spectrometry / Q000295:instrumentation / Q000379:methods* / Q000706:statistics & numerical data; D008969:Molecular Sequence Data; D010455:Peptides / Q000032:analysis / Q000737:chemistry; D011506:Proteins / Q000032:analysis* / Q000737:chemistry; D017421:Sequence Analysis / Q000379:methods
D010455:Peptides; D011506:Proteins