Expression of monocyte chemotactic protein-3 in human monocytes and endothelial cells.
Monocyte chemotactic protein-3 (MCP-3) is a C-C chemokine which interacts with the CCR1, CCR2 (MCP-1) and CCR3 receptors and has a distinct spectrum of action. The present study was designed to investigate whether human endothelial cells (EC) and, by way of comparison, monocytes express MCP-3 and its regulation by pro- and anti-inflammatory cytokines. Unstimulated human umbilical vein EC and monocytes did not express MCP-3. Bacterial lipopolysaccharides (LPS), IL-1 and TNF induced low, but appreciable levels of MCP-3 transcripts. Interferon-gamma was a much weaker, but nevertheless active, inducer. MCP-3 transcripts were considerably less abundant than those for MCP-1. IL-4, IL-13 and IL-10 did not induce MCP-3 expression. These anti-inflammatory cytokines suppressed cytokine-induced MCP-3 expression in monocytes. In contrast, IL-4, IL-13 and IL-10 either did not affect or they amplified MCP-3 induction in EC. EC-produced MCP-3 may be important in the regulation of extravasation of receptor-expressing cells, including NK cells and eosinophils.
Polentarutti
N
N
Department of Immunology and Cell Biology, Istituto di Ricerche Farmacologiche Mario Negri, Milan, Italy.
Introna
M
M
Sozzani
S
S
Mancinelli
R
R
Mantovani
G
G
Mantovani
A
A
D002478:Cells, Cultured; D054410:Chemokine CCL7; D016207:Cytokines / Q000494:pharmacology; D004730:Endothelium, Vascular / Q000276:immunology*; D015870:Gene Expression / Q000187:drug effects; D006801:Humans; D066298:In Vitro Techniques; D007371:Interferon-gamma / Q000494:pharmacology; D007375:Interleukin-1 / Q000494:pharmacology; D008070:Lipopolysaccharides / Q000494:pharmacology; D018945:Monocyte Chemoattractant Proteins / Q000096:biosynthesis / Q000235:genetics*; D009000:Monocytes / Q000276:immunology*; D012333:RNA, Messenger / Q000235:genetics; D011994:Recombinant Proteins; D014409:Tumor Necrosis Factor-alpha / Q000494:pharmacology
C515450:CCL7 protein, human; D054410:Chemokine CCL7; D016207:Cytokines; D007375:Interleukin-1; D008070:Lipopolysaccharides; D018945:Monocyte Chemoattractant Proteins; D012333:RNA, Messenger; D011994:Recombinant Proteins; D014409:Tumor Necrosis Factor-alpha; D007371:Interferon-gamma