Phenotypic and genotypic biotyping of environmental strains of Vibrio cholerae non-O1 isolated in Italy.
The purpose of this study was to characterize strains of Vibrio cholerae non-O1 isolated in Italy from different sources by biochemical and serological assays, antibiotic susceptibility testing, and molecular biotyping. Serotyping and genomic analysis by pulsed-field gel electrophoresis proved to be useful in discriminating the isolates. The data obtained show a wide heterogeneity at the genomic level, and in keeping with this, the serogrouping classification provided evidence of a high variability of the investigated strains. In addition, none of the strains tested produced cholera-like toxins.
Filetici
E
E
Istituto Superiore di Sanità, Rome, Italy.
Bonadonna
L
L
Ciccozzi
M
M
Anastasio
M P
MP
Fantasia
M
M
Shimada
T
T
D000818:Animals; D015373:Bacterial Typing Techniques; D002772:Cholera Toxin / Q000235:genetics; D004269:DNA, Bacterial / Q000235:genetics / Q000302:isolation & purification; D004352:Drug Resistance, Microbial; D016521:Electrophoresis, Gel, Pulsed-Field; D004783:Environmental Microbiology; D005798:Genes, Bacterial; D005838:Genotype; D006801:Humans; D007558:Italy; D010641:Phenotype; D012703:Serotyping; D014734:Vibrio cholerae / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification
D004269:DNA, Bacterial; D002772:Cholera Toxin