Immunological detection of sheep experimentally infected with strains of Mycobacterium avium subspecies containing insertion sequence IS901/IS902 and a 40 kDa protein.
A monoclonal antibody raised against a 40 kDa protein present in certain M. avium strains (IS901/IS902 positive) was used for developing a blocking ELISA. Sera from experimentally infected sheep were evaluated by indirect ELISA, AGID and blocking ELISA. The blocking assay proved to be highly specific for differentiation of sheep infected with different subspecies of M. avium.
Klausen
J
J
Danish Veterinary Laboratory, Copenhagen, Denmark. jk@svs.dk
Pérez
V
V
Giese
S B
SB
García Marín
J F
JF
Ahrens
P
P
D000818:Animals; D000907:Antibodies, Bacterial / Q000097:blood*; D000911:Antibodies, Monoclonal; D004251:DNA Transposable Elements; D004797:Enzyme-Linked Immunosorbent Assay; D016254:Mutagenesis, Insertional; D009162:Mycobacterium avium / Q000235:genetics; D012756:Sheep; D012757:Sheep Diseases; D014376:Tuberculosis / Q000175:diagnosis / Q000382:microbiology / Q000662:veterinary*
D000907:Antibodies, Bacterial; D000911:Antibodies, Monoclonal; D004251:DNA Transposable Elements