title: Transient times in linear metabolic pathways under constant affinity constraints.
abstract: In the early seventies, Easterby began the analytical study of transition times for linear reaction schemes [Easterby (1973) Biochim. Biophys. Acta 293, 552-558]. In this pioneer work and in subsequent papers, a state function (the transient time) was used to measure the period before the stationary state, for systems constrained to work under both constant and variable input flux, was reached. Despite the undoubted usefulness of this quantity to describe the time-dependent features of these kinds of systems, its application to the study of chemical reactions under other constraints is questionable. In the present work, a generalization of these magnitudes to linear metabolic pathways functioning under a constant-affinity constraint is carried out. It is proved that classical definitions of transient times do not reflect the actual properties of the transition to the steady state in systems evolving under this restriction. Alternatively, a more adequate framework for interpretation of the transient times for systems with both constant and variable input flux is suggested. Within this context, new definitions that reflect more accurately the transient characteristics of constant affinity systems are stated. Finally, the meaning of these transient times is discussed.
authors.affiliation: Departamento de Bioquímica y Biología Molecular I, Facultad de Ciencias Químicas, Universidad Complutense, Madrid, 28040 Spain.
authors.forename: M
authors.initials: M
authors.lastname: Lloréns
authors.forename: J C
authors.initials: JC
authors.lastname: Nuño
authors.forename: F
authors.initials: F
authors.lastname: Montero
mesh_terms: D007700:Kinetics; D008433:Mathematics; D008660:Metabolism; D008962:Models, Theoretical; D013997:Time Factors