Insulin-like growth factor-binding protein-1 in human follicular fluid: a marker for oocyte maturation.
In order to investigate the role of insulin-like growth factors (IGFs) in human ovulation, we evaluated the concentrations of IGF-binding proteins (IGFBPs) in human follicular fluid (FF). The concentrations of IGFBP-1 in the FFs of 15 women undergoing in vitro fertilization and embryo transfer were measured and related to those of 17beta-estradiol (E2), progesterone and androstenedione in the FFs. IGFBP-1 levels in the FFs were positively correlated with those of E2 and progesterone. No correlation was found between the IGFBP-1 and androstenedione levels in FFs. The concentrations of IGFBP-1 were significantly increased in the FFs which contained mature oocytes compared with those of immature oocytes, whereas IGFBP-3 in FFs tended to decrease as oocytes matured. It is suggested that IGFs may play important roles in human preovulatory processes, and that IGFBP-1 may be a valuable biochemical marker in the evaluation of oocytes.
Kawano
Y
Y
Department of Obstetrics and Gynecology, Oita Medical University, Japan.
Narahara
H
H
Matsui
N
N
Nasu
K
K
Miyamura
K
K
Miyakawa
I
I
D000735:Androstenedione / Q000378:metabolism; D015415:Biomarkers; D015153:Blotting, Western; D004624:Embryo Transfer; D004797:Enzyme-Linked Immunosorbent Assay; D004958:Estradiol / Q000378:metabolism; D005260:Female; D005307:Fertilization in Vitro; D015571:Follicular Fluid / Q000378:metabolism*; D006801:Humans; D007247:Infertility, Female / Q000378:metabolism / Q000628:therapy; D018970:Insulin-Like Growth Factor Binding Protein 1 / Q000378:metabolism*; D018972:Insulin-Like Growth Factor Binding Protein 3 / Q000378:metabolism; D009865:Oocytes / Q000502:physiology*; D009866:Oogenesis / Q000502:physiology*; D011374:Progesterone / Q000378:metabolism; D011863:Radioimmunoassay
D015415:Biomarkers; D018970:Insulin-Like Growth Factor Binding Protein 1; D018972:Insulin-Like Growth Factor Binding Protein 3; D000735:Androstenedione; D011374:Progesterone; D004958:Estradiol