Contrasting genomic variability between clones from field isolates and laboratory populations of Schistosoma mansoni.
The extent of genomic variability of clones of Schistosoma mansoni obtained from field isolates was compared with that of strains that have been laboratory maintained. Analysis was undertaken using randomly amplified polymorphic DNAs (RAPDs) generated with three primers. Phenograms showing the similarity among the clones were constructed. The data showed that while the laboratory strain is highly homogeneous the clones derived from the field populations were highly variable with 43% of RAPDs exhibiting polymorphisms among 23 clones. Clones isolated from the same infected individual were always more closely grouped than clones from different individuals. The data clearly demonstrated that earlier analyses of the genomic variability in S. mansoni have underestimated this phenomenon due to the failure to examine field isolates.
Pinto
P M
PM
Laboratório de Parasitologia Celular e Molecular, Centro de Pesquisas René Rachou-FIOCRUZ, Belo Horizonte, MG, Brasil.
Brito
C F
CF
Passos
L K
LK
Tendler
M
M
Simpson
A J
AJ
D000818:Animals; D012550:Schistosoma mansoni / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification; D012555:Schistosomiasis mansoni / Q000635:transmission*