Effect of CO on VO2 of carotid body and chemoreception with and without Ca2+.
This study was done using high PCO (> 500 Torr at PO2 of 120 Torr) in the carotid body perfusate in vitro, and recording simultaneously the activity of the whole carotid sinus nerve (CSN) and VO2 of the carotid body. In the cascade of excitation of CSN by high PCO in the dark [light eliminated the excitation; S. Lahiri, News Physiol. Sci. 9 (1992) 161-165], Ca2+ effects occur at the level of neurosecretion after the level of oxygen consumption, according to the following scheme: CO-hypoxia-->VO2 decrease-->K+ conductance decrease-->cell depolarization-->cytosolic Ca2+ rise-->neurosecretion-->neural discharge. Thus, a part of the hypothesis was that [Ca2+] decrease, being a downstream event, may not affect VO2 of the carotid body. Also, to determine to what extent the intracellular calcium stores contribute to cystolic [Ca2+] and chemosensory discharge with high PCO, we tested the effect of interruption of perfusate flow with medium nominally free of [Ca2+] on CSN excitation and VO2 of the carotid body with and without high PCO. High PCO in the dark decreased carotid body VO2, independent of [Ca2+]o. CSN excitation was always enhanced by high PCO, and its sensitivity to perfusate flow interruption. Also, nominally Ca(2+)-free solution increased the latency and decreased the rate of rise and peak activity of CSN during interruption of perfusate flow, but CO augmented the responses. This reversal effect by CO suggests that Ca2+ is released from intracellular stores, because CO has no other way to excite the chemoreceptors than by acting on the intracellular stores.
Lahiri
S
S
Department of Physiology, University of Pennsylvania School of Medicine, Philadelphia 19104, USA. lahiri@mail.med.upenn.edu
Buerk
D G
DG
Osanai
S
S
Mokashi
A
A
Chugh
D K
DK
D000818:Animals; D002118:Calcium / Q000494:pharmacology*; D002248:Carbon Monoxide / Q000494:pharmacology*; D002344:Carotid Body / Q000187:drug effects / Q000378:metabolism*; D002415:Cats; D002628:Chemoreceptor Cells / Q000187:drug effects / Q000378:metabolism*; D004594:Electrophysiology; D000860:Hypoxia / Q000378:metabolism; D066298:In Vitro Techniques; D007700:Kinetics; D008027:Light; D009474:Neurons / Q000187:drug effects / Q000502:physiology; D010101:Oxygen Consumption / Q000187:drug effects*
D002248:Carbon Monoxide; D002118:Calcium