title: Experimental treatment of cutaneous leishmaniasis with argentilactone isolated from Annona haematantha.
abstract: From the hexanic extract of roots of Annona haematantha an alpha,beta-unsaturated delta-lactone was isolated and identified as argentilactone. This compound exhibited in vitro activity against various strains of Leishmania ssp. at 10 micrograms/ml. BALB/c mice infected with Leishmania amazonensis were treated four weeks after infection with argentilactone by oral or subcutaneous routes for 14 days at 25 mg/kg daily. The reference drug, N-methylglucamine antimonate, was administered by subcutaneous injections at 100 mg/kg for 14 days. In these conditions, argentilactone showed the same efficacy as the reference drug, reducing by 96% the parasite loads in the lesion and by 50% the parasite burden in spleen.
authors.affiliation: Laboratoire de Pharmacognosie, BIOCIS URA 1843 CNRS, Faculté de Pharmacie, Châtenay-Malabry, France.
authors.forename: A I
authors.initials: AI
authors.lastname: Waechter
authors.forename: M E
authors.initials: ME
authors.lastname: Ferreira
authors.forename: A
authors.initials: A
authors.lastname: Fournet
authors.forename: A
authors.initials: A
authors.lastname: Rojas de Arias
authors.forename: H
authors.initials: H
authors.lastname: Nakayama
authors.forename: S
authors.initials: S
authors.lastname: Torres
authors.forename: R
authors.initials: R
authors.lastname: Hocquemiller
authors.forename: A
authors.initials: A
authors.lastname: Cavé
mesh_terms: D000818:Animals; D005260:Female; D005616:French Guiana; D007783:Lactones / Q000737:chemistry / Q000302:isolation & purification / Q000627:therapeutic use*; D007894:Leishmania mexicana / Q000302:isolation & purification; D016773:Leishmaniasis, Cutaneous / Q000188:drug therapy* / Q000473:pathology / Q000503:physiopathology; D008297:Male; D016488:Medicine, African Traditional; D051379:Mice; D008807:Mice, Inbred BALB C; D010946:Plants, Medicinal; D014344:Trypanocidal Agents / Q000737:chemistry / Q000302:isolation & purification / Q000627:therapeutic use*
chemical_list: D007783:Lactones; D014344:Trypanocidal Agents; C108954:argentilactone