title: Biodosimetry results from space flight Mir-18.
abstract: Astronauts are classified as radiation workers due to the presence of ionizing radiation in space. For the assessment of health risks, physical dosimetry has been indispensable. However, the change of the location of dosimeters on the crew members, the variation in dose rate with location inside the spacecraft and the unknown biological effects of microgravity can introduce significant uncertainties in estimating exposure. To circumvent such uncertainty, a study on the cytogenetic effects of space radiation in human lymphocytes was proposed and conducted for Mir-18, a 115-day mission. This study used fluorescence in situ hybridization (FISH) with whole-chromosome painting probes to score chromosomal exchanges and the Giemsa staining method to determine the frequency of dicentrics. The growth kinetics of cells and sister chromatid exchanges (SCEs) were examined to ensure that chromosomal aberrations were scored in the first mitosis and were induced primarily by space radiation. Our results showed that the frequency of chromosomal aberrations increased significantly in postflight samples compared to samples drawn prior to flight, and that the frequency of SCEs was similar for both pre- and postflight samples. Based on a dose-response curve for preflight samples exposed to gamma rays, the absorbed dose received by crew members during the mission was estimated to be about 14.75 cSv. Because the absorbed dose measured by physical dosimeters is 5.2 cGy for the entire mission, the RBE is about 2.8.
authors.affiliation: NASA Johnson Space Center, Houston, Texas 77058, USA.
authors.forename: T C
authors.initials: TC
authors.lastname: Yang
authors.forename: K
authors.initials: K
authors.lastname: George
authors.forename: A S
authors.initials: AS
authors.lastname: Johnson
authors.forename: M
authors.initials: M
authors.lastname: Durante
authors.forename: B S
authors.initials: BS
authors.lastname: Fedorenko
mesh_terms: D018480:Astronauts; D002869:Chromosome Aberrations; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D008214:Lymphocytes; D011829:Radiation Dosage; D011834:Radiation Monitoring; D012426:Russia; D012854:Sister Chromatid Exchange; D013026:Space Flight