Mapping histology to metabolism: coregistration of stained whole-brain sections to premortem PET in Alzheimer's disease.
The association between [18F]fluorodeoxyglucose positron emission tomography (FDG-PET) counts obtained 8 h before death and neurofibrillary tangle (NFT) staining density in a patient with Alzheimer's disease (AD) was evaluated. In our patient FDG-PET counts were globally decreased with a greater focal deficit in the left medial temporal region independent of volume loss. After death, whole-brain sections derived from cryomacrotome sectioning were stained for NFTs by the Gallyas method and elastically warped into their native space enabling registration with premortem FDG-PET data. Gallyas staining density was localized to the paralimbic cortex of the basal forebrain, medial temporal, and orbital frontal regions. The poor correlation between NFT staining density and hypometabolism on FDG-PET implicates alternate mechanisms underlying the metabolic defect in AD.
Mega
M S
MS
Department of Neurology, UCLA School of Medicine 90024, USA.
Chen
S S
SS
Thompson
P M
PM
Woods
R P
RP
Karaca
T J
TJ
Tiwari
A
A
Vinters
H V
HV
Small
G W
GW
Toga
A W
AW
D000368:Aged; D000369:Aged, 80 and over; D000544:Alzheimer Disease / Q000473:pathology* / Q000503:physiopathology; D001786:Blood Glucose / Q000378:metabolism*; D001921:Brain / Q000473:pathology* / Q000503:physiopathology; D001931:Brain Mapping; D004734:Energy Metabolism / Q000502:physiology*; D019788:Fluorodeoxyglucose F18; D006801:Humans; D007091:Image Processing, Computer-Assisted; D008297:Male; D016874:Neurofibrillary Tangles / Q000473:pathology / Q000502:physiology; D015203:Reproducibility of Results; D014055:Tomography, Emission-Computed
D001786:Blood Glucose; D019788:Fluorodeoxyglucose F18