Immobilization stress increases mRNA levels of interleukin-1 receptor antagonist in various rat brain regions.
1. Interleukin-1 receptor antagonist (IL-1Ra), as well as the interleukin-1 beta (IL-1 beta) gene response to immobilization stress (IMS), was examined in the rat brain. The reverse transcription-polymerase chain reaction was employed to determine mRNA levels. 3. IL-1 beta and IL-1Ra mRNA levels peaked at approximately 0.5 and 2-4 hr, respectively. The maximum mRNA levels of IL-1 beta were 15-fold higher than pre-IMS levels, whereas those of IL-1Ra were 250-fold higher in the hypothalamus. 3. After the biosynthesis of IL-1 beta has peaked, IL-1Ra may contribute to attenuation of the IL-1 activity which has been enhanced by IMS.
Suzuki
E
E
Department of Neuropsychiatry, Keio University School of Medicine, Tokyo, Japan.
Shintani
F
F
Kanba
S
S
Asai
M
M
Nakaki
T
T
D000818:Animals; D001921:Brain / Q000503:physiopathology*; D001923:Brain Chemistry / Q000502:physiology*; D015870:Gene Expression / Q000502:physiology; D053590:Interleukin 1 Receptor Antagonist Protein; D008297:Male; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D017472:Receptors, Interleukin-1 / Q000037:antagonists & inhibitors / Q000502:physiology; D012149:Restraint, Physical; D012795:Sialoglycoproteins / Q000235:genetics*; D015398:Signal Transduction / Q000502:physiology; D013312:Stress, Physiological / Q000503:physiopathology*
D053590:Interleukin 1 Receptor Antagonist Protein; D012333:RNA, Messenger; D017472:Receptors, Interleukin-1; D012795:Sialoglycoproteins