Oxygen radicals diminish dopamine transporter function in rat striatum.
Incubation of striatal synaptosomes with the oxygen radical generating enzyme, xanthine oxidase, decreased [3H]dopamine uptake: an effect attributable to a decreased Vmax. Concurrent incubation with the superoxide radical scavenger, superoxide dismutase, abolished the xanthine oxidase-induced decrease. These results indicate that, like methamphetamine administration in vivo, reactive oxygen species diminish dopamine transporter function in vitro. The significance of these findings to mechanisms responsible for effects of methamphetamine is discussed.
Fleckenstein
A E
AE
Department of Pharmacology and Toxicology, University of Utah, Salt Lake City 84112, USA.
Metzger
R R
RR
Beyeler
M L
ML
Gibb
J W
JW
Hanson
G R
GR
D000818:Animals; D003342:Corpus Striatum / Q000378:metabolism*; D004298:Dopamine / Q000378:metabolism*; D018765:Dopamine Uptake Inhibitors / Q000494:pharmacology*; D008297:Male; D008694:Methamphetamine / Q000494:pharmacology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D017382:Reactive Oxygen Species / Q000378:metabolism*; D013482:Superoxide Dismutase / Q000378:metabolism; D014969:Xanthine Oxidase / Q000378:metabolism
D018765:Dopamine Uptake Inhibitors; D017382:Reactive Oxygen Species; D008694:Methamphetamine; D013482:Superoxide Dismutase; D014969:Xanthine Oxidase; D004298:Dopamine