title: Molecular diagnosis of B- and T-cell lymphomas: fundamental principles and clinical applications.
abstract: Molecular diagnostic assays have become routine in the evaluation of lymphoid malignancies. Both Southern transfer and polymerase chain reaction (PCR) technologies are used to assess for B- and T-cell clonality, the presence of rearrangements involving protooncogenes such as bcl-1 and bcl-2, and the monitoring of minimal residual disease. We review the fundamentals of B- and T-cell ontogeny as well as the basic principles of the Southern transfer and PCR assays and their applications to the diagnosis of lymphoid malignancies.
authors.affiliation: Department of Pathology and Laboratory Medicine, Hartford Hospital, CT 06102, USA.
authors.forename: W N
authors.initials: WN
authors.lastname: Rezuke
authors.forename: E C
authors.initials: EC
authors.lastname: Abernathy
authors.forename: G J
authors.initials: GJ
authors.lastname: Tsongalis
mesh_terms: D015139:Blotting, Southern; D004273:DNA, Neoplasm / Q000235:genetics; D015329:Gene Rearrangement, T-Lymphocyte; D006801:Humans; D007136:Immunoglobulins / Q000235:genetics; D016130:Immunophenotyping; D016393:Lymphoma, B-Cell / Q000175:diagnosis* / Q000235:genetics / Q000276:immunology; D016399:Lymphoma, T-Cell / Q000175:diagnosis* / Q000235:genetics / Q000276:immunology; D014178:Translocation, Genetic
chemical_list: D004273:DNA, Neoplasm; D007136:Immunoglobulins