Presence of mu and kappa opioid receptor mRNAs in galanin but not in GnRH neurons in the female rat.
Using a combination of radioactive and non-radioactive in situ hybridizations, the expression of mu and kappa opioid receptor mRNA was investigated in neurons in the female rat preoptic nucleus expressing galanin and gonadotropin-releasing hormone (GnRH) mRNA. Numerous cells expressing both mu or kappa and galanin were found in the intermediate and rostral regions of the preoptic area whereas little co-localization was observed at the rostral level. The number of kappa/galanin expressing cells was greater than that of mu/galanin cells. mu/galanin co-localization was observed essentially in the anteroventral preoptic nucleus while neurons expressing kappa/galanin were present in both the anteroventral preoptic nucleus and in the periventricular hypothalamic nucleus. Co-localization of GnRH with mu or kappa could not be detected in the preoptic area. These observations showed that galaninergic neurons but not GnRH neurons of the preoptic area might be directly regulated by endogenous opioid peptides.
Mitchell
V
V
INSERM U422, Lille, France.
Prevot
V
V
Jennes
L
L
Aubert
J P
JP
Croix
D
D
Beauvillain
J C
JC
D000818:Animals; D005260:Female; D019004:Galanin / Q000032:analysis*; D007987:Gonadotropin-Releasing Hormone / Q000032:analysis*; D009474:Neurons / Q000737:chemistry / Q000378:metabolism*; D012333:RNA, Messenger / Q000032:analysis*; D051381:Rats; D017207:Rats, Sprague-Dawley; D017473:Receptors, Opioid, kappa / Q000235:genetics*; D017450:Receptors, Opioid, mu / Q000235:genetics*
D012333:RNA, Messenger; D017473:Receptors, Opioid, kappa; D017450:Receptors, Opioid, mu; D007987:Gonadotropin-Releasing Hormone; D019004:Galanin