Humoral immune response against Campylobacter jejuni lipopolysaccharides in Guillain-Barré and Miller Fisher syndrome.
In this study we characterized the IgG antibodies against lipopolysaccharides (LPS) of Campylobacter jejuni in serum from patients with Guillain-Barré syndrome (GBS), Miller Fisher syndrome (MFS), C. jejuni enteritis and normal controls. In patients with GBS and MFS long-lasting titers of IgG1 and IgG3 antibodies against LPS from GBS and MFS associated C. jejuni were found. The subclass and course of these antibodies were highly associated with those of antibodies against GM1 and GQ1b in GBS and MFS patients. However, in C. jejuni enteritis and normal controls anti-LPS antibodies were predominantly IgG2. Antibody binding with LPS was reduced after treatment with choleratoxin and sialidases, suggesting that the ganglioside-like epitopes in LPS are immunodominant. These results further indicate that antecedent C. jejuni infections determine the specificity and isotype of anti-ganglioside antibodies in GBS and MFS patients.
Jacobs
B C
BC
Department of Neurology, Erasmus University, Rotterdam, The Netherlands.
Endtz
H P
HP
van der Meché
F G
FG
Hazenberg
M P
MP
de Klerk
M A
MA
van Doorn
P A
PA
D000906:Antibodies / Q000145:classification / Q000276:immunology; D000917:Antibody Formation; D000918:Antibody Specificity / Q000276:immunology; D016123:Campylobacter jejuni / Q000378:metabolism*; D005677:G(M1) Ganglioside / Q000276:immunology; D005732:Gangliosides / Q000276:immunology; D006801:Humans; D007074:Immunoglobulin G / Q000032:analysis / Q000276:immunology; D008070:Lipopolysaccharides / Q000276:immunology* / Q000378:metabolism*; D019846:Miller Fisher Syndrome / Q000276:immunology*; D011129:Polyradiculoneuropathy / Q000276:immunology*
D000906:Antibodies; D005732:Gangliosides; D007074:Immunoglobulin G; D008070:Lipopolysaccharides; D005677:G(M1) Ganglioside; C039129:GQ1b ganglioside