Rapid characterization of human chromosomes in hybrid cell lines by primed in situ (PRINS) labeling.
The primed in situ (PRINS) labeling technique allows a rapid and specific labeling of human chromosomes in situ. This method is based on annealing of specific oligonucleotide primers and subsequent primer extension by a Taq DNA polymerase. We have developed a PRINS protocole for the cytogenetic analysis of somatic hybrid cell lines. Painting of human chromosomes is performed using Alu specific primers. Individual human chromosomes are identified using chromosome-specific alpha-satellite primers. The method was successfully tested to 3 different human-hamster hybrid cell lines. This approach provides an interesting alternative to classical cytogenetic and in situ hybridization techniques for the characterization of the human content of hybrid cell lines.
Coullin
P
P
INSERM U178, Villejuif, France.
Pellestor
F
F
D000818:Animals; D002460:Cell Line; D002875:Chromosomes / Q000737:chemistry*; D002895:Chromosomes, Human, Pair 5 / Q000737:chemistry; D006224:Cricetinae; D017931:DNA Primers; D006801:Humans; D006822:Hybrid Cells / Q000737:chemistry*; D017404:In Situ Hybridization, Fluorescence; D012091:Repetitive Sequences, Nucleic Acid
D017931:DNA Primers