title: Pretreatment prediction of the chemotherapeutic response of human glioma cell cultures using nuclear magnetic resonance spectroscopy and artificial neural networks.
abstract: Both tumor metabolism and its response to cytotoxic drugs are intrinsic properties of tumor cells. It is therefore likely that there is a relationship between the two properties, however subtle and complex, wherein the metabolic characteristics of tumor cells can reflect the inherent response (resistance or sensitivity) of these cells to cytotoxic drugs. We used artificial neural network analysis to show that it is possible to distinguish, prior to treatment, between drug-resistant and drug-sensitive human glioma cell cultures from their metabolic profiles, as given by high-resolution proton nuclear magnetic resonance spectra of the cell extracts, and to predict their cellular response to the chemotherapeutic drug 1-(2-chloroethyl)-3-cyclohexyl-1-nitrosourea in vitro. The results suggest that neural network analysis of tumor nuclear magnetic resonance spectra has potential as a prognostic tool for determining treatment of gliomas, ultimately noninvasively, and may be used to provide information about the metabolic pathways involved in drug response that may be helpful in developing novel treatments for these tumors.
authors.affiliation: Department of Neurological Surgery, Institute of Neurology, London, United Kingdom. W.ElDeredy@ion.ucl.ac.uk
authors.forename: W
authors.initials: W
authors.lastname: El-Deredy
authors.forename: S M
authors.initials: SM
authors.lastname: Ashmore
authors.forename: N M
authors.initials: NM
authors.lastname: Branston
authors.forename: J L
authors.initials: JL
authors.lastname: Darling
authors.forename: S R
authors.initials: SR
authors.lastname: Williams
authors.forename: D G
authors.initials: DG
authors.lastname: Thomas
mesh_terms: D018906:Antineoplastic Agents, Alkylating / Q000494:pharmacology* / Q000627:therapeutic use; D001932:Brain Neoplasms / Q000188:drug therapy* / Q000378:metabolism / Q000473:pathology; D002455:Cell Division; D019008:Drug Resistance, Neoplasm; D004354:Drug Screening Assays, Antitumor / Q000379:methods*; D005910:Glioma / Q000188:drug therapy* / Q000378:metabolism / Q000473:pathology; D006801:Humans; D008130:Lomustine / Q000494:pharmacology / Q000627:therapeutic use; D009682:Magnetic Resonance Spectroscopy; D016571:Neural Networks, Computer; D014407:Tumor Cells, Cultured / Q000187:drug effects
chemical_list: D018906:Antineoplastic Agents, Alkylating; D008130:Lomustine