Extensive genomic conservation of cattle microsatellite heterozygosity in sheep.
We report the evaluation of 1036 bovine microsatellite primer pairs for their suitability as linkage markers in sheep. Approximately 58% (605/1036) of bovine primer pairs amplified a locus in sheep. Sixty-seven per cent (409/605) of amplified loci were detected as polymorphic. Marker heterozygosity, allele number and range of allele sizes were significantly lower in sheep than cattle sampled in this study. However, median fragment size was similar. These data suggest that high-resolution comparative linkage maps between closely related species can be constructed relatively efficiently.
de Gortari
M J
MJ
USDA, ARS U.S. Meat Animal Research Center, Clay Center, NE 68933, USA.
Freking
B A
BA
Kappes
S M
SM
Leymaster
K A
KA
Crawford
A M
AM
Stone
R T
RT
Beattie
C W
CW
D000483:Alleles; D000818:Animals; D002417:Cattle / Q000235:genetics*; D017124:Conserved Sequence; D017931:DNA Primers; D019143:Evolution, Molecular; D016678:Genome; D006579:Heterozygote; D018895:Microsatellite Repeats; D016133:Polymerase Chain Reaction; D012756:Sheep / Q000235:genetics*; D013045:Species Specificity
D017931:DNA Primers