Glucocorticoids stimulate growth of human papillomavirus type 16 (HPV16)-immortalized human keratinocytes and support HPV16-mediated immortalization without affecting the levels of HPV16 E6/E7 mRNA.
We investigated the effects of the glucocorticoids hydrocortisone and dexamethasone on human papillomavirus type 16 (HPV16)-mediated human cell carcinogenesis using normal human keratinocytes (HKc) and HKc immortalized by transfection with HPV16 DNA (HKc/HPV16). Normal HKc did not require glucocorticoids for proliferation. In contrast, growth of early passage HKc/HPV16 strictly required these hormones, although glucocorticoid dependence became less stringent during in vitro progression. Glucocorticoid dependence was acquired by HKc early after immortalization with HPV16 DNA, and glucocorticoids were required for efficient HKc immortalization. However, treatment of HKc/HPV16 with hydrocortisone or dexamethasone did not increase the steady-state levels of HPV16 E6/E7 mRNA or protein. Firefly luciferase activity expressed under the control of the HPV16 upstream regulatory region and P97 promoter increased by about fourfold following dexamethasone treatment of HeLa, but only twofold in HKc/HPV16, and less than twofold in SiHa. However, all of these cell lines expressed sufficient endogenous glucocorticoid receptors to allow for a dexamethasone response of the mouse mammary tumor virus promoter. These results indicate that mechanisms other than a direct influence by glucocorticoids on HPV16 early gene expression may contribute to the striking biological effects of these steroids on HPV16-mediated human cell carcinogenesis.
Khan
M A
MA
Department of Pathology, University of South Carolina School of Medicine, Columbia, USA.
Canhoto
A J
AJ
Housley
P R
PR
Creek
K E
KE
Pirisi
L
L
D000818:Animals; D000893:Anti-Inflammatory Agents / Q000494:pharmacology*; D002455:Cell Division / Q000187:drug effects; D002472:Cell Transformation, Viral / Q000187:drug effects*; D002478:Cells, Cultured; D001517:Coleoptera; D003907:Dexamethasone / Q000494:pharmacology*; D015536:Down-Regulation / Q000235:genetics; D015967:Gene Expression Regulation, Viral / Q000187:drug effects; D017930:Genes, Reporter; D005938:Glucocorticoids / Q000494:pharmacology*; D006133:Growth Substances / Q000502:physiology; D006367:HeLa Cells; D006801:Humans; D006854:Hydrocortisone / Q000494:pharmacology*; D015603:Keratinocytes / Q000737:chemistry / Q000166:cytology* / Q000821:virology; D008156:Luciferases / Q000235:genetics; D027383:Papillomaviridae / Q000235:genetics*; D011401:Promoter Regions, Genetic / Q000502:physiology; D012333:RNA, Messenger / Q000378:metabolism; D012367:RNA, Viral / Q000378:metabolism; D011965:Receptors, Glucocorticoid / Q000032:analysis / Q000378:metabolism; D012867:Skin / Q000166:cytology; D014764:Viral Proteins / Q000235:genetics / Q000378:metabolism
D000893:Anti-Inflammatory Agents; D005938:Glucocorticoids; D006133:Growth Substances; D012333:RNA, Messenger; D012367:RNA, Viral; D011965:Receptors, Glucocorticoid; D014764:Viral Proteins; D003907:Dexamethasone; D008156:Luciferases; D006854:Hydrocortisone