Advanced glycation endproducts stimulate mitogen-activated protein kinase and proliferation in rabbit vascular smooth muscle cells.
Advanced glycation end products of bovine serum albumin (AGEs-BSA) exhibited biphasic effects on the proliferation of cultured rabbit vascular smooth muscle cells (VSMCs) in terms of [3H]thymidine incorporation and cell number count; a stimulatory effect was observed at 1-10 micrograms/ml and an inhibitory effect at more than 20 micrograms/ml, while it inhibited [3H]thymidine incorporation even at 1-10 micrograms/ml in cultured bovine vascular endothelial cells (VECs). Transient activation of p42 mitogen-activated protein kinase (MAPK) with a peak at around 5 min and a subsequent sustained phase was induced by AGEs-BSA in VSMCs, but not in VECs. The dependence of MAPK activation on AGEs-BSA dose was correlated with that of VSMCs proliferation.
Satoh
H
H
First Department of Internal Medicine, Faculty of Medicine, University of Tokyo, Japan.
Togo
M
M
Hara
M
M
Miyata
T
T
Han
K
K
Maekawa
H
H
Ohno
M
M
Hashimoto
Y
Y
Kurokawa
K
K
Watanabe
T
T
D000818:Animals; D017871:Calcium-Calmodulin-Dependent Protein Kinases / Q000378:metabolism*; D002417:Cattle; D002455:Cell Division; D002478:Cells, Cultured; D004730:Endothelium, Vascular / Q000166:cytology / Q000378:metabolism; D017127:Glycation End Products, Advanced / Q000494:pharmacology*; D009131:Muscle, Smooth, Vascular / Q000166:cytology / Q000201:enzymology / Q000378:metabolism*; D011817:Rabbits; D013379:Substrate Specificity; D013936:Thymidine / Q000378:metabolism
D017127:Glycation End Products, Advanced; D017871:Calcium-Calmodulin-Dependent Protein Kinases; D013936:Thymidine