title: Anti-inflammatory activity of superoxide dismutase conjugated with sodium hyaluronate.
abstract: Superoxide dismutase (SOD) from bovine erythrocytes was conjugated with sodium hyaluronate (HA) with a mean molecular weight of 10(6) to have greater anti-inflammatory activity in vivo. Amino groups of SOD were coupled with carboxyl groups in the hyaluronate molecule using 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide. The HA-SOD conjugate was composed of 1.5 mol of SOD molecule per 1 mol of hyaluronate on the average, and retained 70% of the activity of unmodified SOD. The conjugate was essentially non-immunogenic in mice, and exhibited much higher anti-inflammatory activities than HA or SOD in models of inflammatory diseases such as ischemic oedema of the foot-pad in mice, carrageenin-induced pleurisy and adjuvant arthritis in rats.
authors.affiliation: Toin Human Science and Technology Centre, Department of Materials Science and Technology, Toin University of Yokohama, Japan.
authors.forename: K
authors.initials: K
authors.lastname: Sakurai
authors.forename: K
authors.initials: K
authors.lastname: Miyazaki
authors.forename: Y
authors.initials: Y
authors.lastname: Kodera
authors.forename: H
authors.initials: H
authors.lastname: Nishimura
authors.forename: M
authors.initials: M
authors.lastname: Shingu
authors.forename: Y
authors.initials: Y
authors.lastname: Inada
mesh_terms: D000818:Animals; D000893:Anti-Inflammatory Agents / Q000737:chemistry / Q000494:pharmacology* / Q000627:therapeutic use; D001169:Arthritis, Experimental / Q000188:drug therapy; D002351:Carrageenan / Q000633:toxicity; D002417:Cattle; D005260:Female; D006820:Hyaluronic Acid / Q000737:chemistry*; D008297:Male; D051379:Mice; D010998:Pleurisy / Q000139:chemically induced / Q000188:drug therapy; D051381:Rats; D017207:Rats, Sprague-Dawley; D013482:Superoxide Dismutase / Q000737:chemistry*
chemical_list: D000893:Anti-Inflammatory Agents; D002351:Carrageenan; D006820:Hyaluronic Acid; D013482:Superoxide Dismutase