Characterization of the early region 4 of porcine adenovirus type 3.
The nucleotide sequence of a 3028 bp DNA segment, located between map co-ordinates 100 and 92 in the genome of porcine adenovirus type 3 (PAV-3), was determined. The segment includes the entire early region 4 (E-4) and the right inverted terminal repeat sequences. There were two TATA boxes and one canonical polyadenylation signal on the 1 strand. Homology searches of the GenBank data base for the predicted amino acid sequences revealed that, of the eight open reading frames (ORFs) on the 1 strand, and four ORFs on the r strand, only ORF 8 on the 1 strand showed homology with the 34 kDa E-4 protein of human adenovirus types 2, 12 and 34. Northern blot analysis showed that transcription from the E-4 region of PAV-3 began 4 h after infection, peaked at 8 h and declined after 10 h, before DNA replication began 16 h after infection. The E-4 region of PAV-3 was further characterized by 5' and 3' end mapping of the transcription unit.
Reddy
P S
PS
Department of Pathobiology, University of Guelph, Canada.
Idamakanti
N
N
Derbyshire
J B
JB
Nagy
E
E
D000256:Adenoviridae / Q000235:genetics*; D017504:Adenovirus E4 Proteins / Q000235:genetics*; D000818:Animals; D002874:Chromosome Mapping; D016679:Genome, Viral; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D012333:RNA, Messenger / Q000032:analysis; D017422:Sequence Analysis, DNA; D013552:Swine / Q000821:virology*; D016385:TATA Box; D014764:Viral Proteins / Q000235:genetics
D017504:Adenovirus E4 Proteins; D012333:RNA, Messenger; D014764:Viral Proteins