Detection of nucleoprotein gene of Sendai virus in the lungs of rats by touchdown nested reverse transcription polymerase chain reaction.
The nucleoprotein (NP) gene of Sendai virus was detected by touchdown nested reverse transcription polymerase chain reaction (RT-PCR) in the lungs of a rat presented with respiratory illness and high serum ELISA titer to Sendai virus. This method seemed to be of value in controlling infection in laboratory rodents.
Hayase
Y
Y
Department of Virology, Jichi Medical School, Tochigi, Japan.
Tobita
K
K
Kii
M
M
Hakamada
Y
Y
Arai
T
T
D000595:Amino Acid Sequence; D000818:Animals; D000830:Animals, Laboratory; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D005814:Genes, Viral / Q000235:genetics*; D008171:Lung Diseases / Q000175:diagnosis; D008969:Molecular Sequence Data; D009698:Nucleoproteins / Q000235:genetics*; D016133:Polymerase Chain Reaction; D051381:Rats; D018108:Respirovirus / Q000235:genetics*; D010253:Respirovirus Infections / Q000175:diagnosis* / Q000235:genetics
D009698:Nucleoproteins