Heterologous expression of human drug-metabolizing enzymes.
This article is a report on a symposium held at the March 1997 meeting of the American Society for Pharmacology and Experimental Therapeutics in San Diego. Current developments in the heterologous expression of cytochrome P450, NADPH-cytochrome P450 reductase, glutathione transferase, and UDP-glucuronosyltransferase enzymes are described. Systems include bacteria, insect cells, and transient and stable mammalian cells. Uses of the products are described for discernment of which enzymes are involved in metabolism of drugs, genotoxicity assays, mutagenesis (for structure-activity relationships), large scale production of enzyme products, antibody production, and production of proteins for biophysical studies.
Guengerich
F P
FP
Department of Biochemistry and Center in Molecular Toxicology, Vanderbilt University School of Medicine.
Parikh
A
A
Johnson
E F
EF
Richardson
T H
TH
von Wachenfeldt
C
C
Cosme
J
J
Jung
F
F
Strassburg
C P
CP
Manns
M P
MP
Tukey
R H
RH
Pritchard
M
M
Fournel-Gigleux
S
S
Burchell
B
B
D004798:Enzymes / Q000096:biosynthesis / Q000737:chemistry / Q000378:metabolism*; D006801:Humans; D004364:Pharmaceutical Preparations / Q000378:metabolism*; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000737:chemistry / Q000378:metabolism
D004798:Enzymes; D004364:Pharmaceutical Preparations; D011994:Recombinant Proteins