Experimental treatment of cutaneous leishmaniasis with argentilactone isolated from Annona haematantha.
From the hexanic extract of roots of Annona haematantha an alpha,beta-unsaturated delta-lactone was isolated and identified as argentilactone. This compound exhibited in vitro activity against various strains of Leishmania ssp. at 10 micrograms/ml. BALB/c mice infected with Leishmania amazonensis were treated four weeks after infection with argentilactone by oral or subcutaneous routes for 14 days at 25 mg/kg daily. The reference drug, N-methylglucamine antimonate, was administered by subcutaneous injections at 100 mg/kg for 14 days. In these conditions, argentilactone showed the same efficacy as the reference drug, reducing by 96% the parasite loads in the lesion and by 50% the parasite burden in spleen.
Waechter
A I
AI
Laboratoire de Pharmacognosie, BIOCIS URA 1843 CNRS, Faculté de Pharmacie, Châtenay-Malabry, France.
Ferreira
M E
ME
Fournet
A
A
Rojas de Arias
A
A
Nakayama
H
H
Torres
S
S
Hocquemiller
R
R
Cavé
A
A
D000818:Animals; D005260:Female; D005616:French Guiana; D007783:Lactones / Q000737:chemistry / Q000302:isolation & purification / Q000627:therapeutic use*; D007894:Leishmania mexicana / Q000302:isolation & purification; D016773:Leishmaniasis, Cutaneous / Q000188:drug therapy* / Q000473:pathology / Q000503:physiopathology; D008297:Male; D016488:Medicine, African Traditional; D051379:Mice; D008807:Mice, Inbred BALB C; D010946:Plants, Medicinal; D014344:Trypanocidal Agents / Q000737:chemistry / Q000302:isolation & purification / Q000627:therapeutic use*
D007783:Lactones; D014344:Trypanocidal Agents; C108954:argentilactone