A Drosophila muscle-specific gene related to the mouse quaking locus.
We have characterized a novel muscle-specific gene of Drosophila melanogaster, defined by enhancer trap strain 24B of Brand and Perrimon (1993). We show that transcripts of the gene accumulate within presumptive mesoderm and persist within developing muscles, strongly suggesting that the encoded protein is involved in muscle cell determination and differentiation. cDNA sequences reveal that the Drosophila protein is similar to quaking (64% identity over 210 amino acids), a protein essential for mouse embryogenesis, and gld-1 (53% identity over 162 amino acids) a germ-line-specific tumor suppressing protein of the nematode, Caenorhabditis elegans. We demonstrate that the Drosophila gene resides within the 93F chromosome subdivision, and describe its physical map. Finally, we have used the gene, which we have named quaking-related 93F (qkr93F), to identify a family of closely related KH domains.
Fyrberg
C
C
Department of Biology, Johns Hopkins University, Baltimore, MD 21218, USA.
Becker
J
J
Barthmaier
P
P
Mahaffey
J
J
Fyrberg
E
E
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D029742:Caenorhabditis elegans Proteins; D002454:Cell Differentiation; D002874:Chromosome Mapping; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics; D029721:Drosophila Proteins; D004331:Drosophila melanogaster / Q000196:embryology / Q000235:genetics*; D018507:Gene Expression Regulation, Developmental; D017344:Genes, Insect / Q000235:genetics*; D015801:Helminth Proteins / Q000235:genetics; D008648:Mesoderm / Q000737:chemistry; D051379:Mice; D008969:Molecular Sequence Data; D005810:Multigene Family / Q000235:genetics; D009132:Muscles / Q000737:chemistry / Q000166:cytology; D009687:Nuclear Proteins; D012333:RNA, Messenger / Q000032:analysis; D016601:RNA-Binding Proteins / Q000235:genetics*; D017386:Sequence Homology, Amino Acid
D029742:Caenorhabditis elegans Proteins; D018076:DNA, Complementary; D029721:Drosophila Proteins; C094448:GLD-1 protein, C elegans; D015801:Helminth Proteins; D009687:Nuclear Proteins; C098217:Qk protein, mouse; D012333:RNA, Messenger; D016601:RNA-Binding Proteins; C106296:how protein, Drosophila