Serum levels of soluble tumor-necrosis-factor receptors in patients with benign and malignant HPV-associated anogenital lesions.
The levels of type-I and type-II soluble TNF-alpha receptors (sTNF-Rs) were evaluated in sera from patients with various human-papillomavirus-(HPV)-associated benign and malignant anogenital lesions using specific enzyme-linked immunobiological assays. In patients with benign HPV6/11-associated condylomata acuminata, the levels of sTNF-RI were significantly increased, while sTNF-RII were in normal range. Both types of sTNF-Rs were in normal range in patients with benign HPV16-associated grade-I/II and grade-III cervical intra-epithelial neoplasia. However, their levels were significantly increased in patients with HPV16/18-associated squamous cervical cancer and anogenital Bowen's carcinoma. Sera from patients with condylomata acuminata and anogenital carcinomas displayed significantly increased TNF-alpha-inhibitory activity, as revealed by L929 cell-cytotoxicity assay. Increased serum TNF-alpha-inhibitory activity correlated with higher levels of sTNF-Rs. Furthermore, this inhibitory activity could be specifically abrogated by htr9 and utr1 monoclonal antibodies recognizing TNF-RI and TNF-RII respectively. Our results strongly suggest that serum sTNF-Rs may protect tumor cells from cytotoxic/cytostatic effects of locally released TNF-alpha, and that elevated levels of circulating sTNF-Rs may facilitate the growth of HPV-associated anogenital lesions.
Malejczyk
M
M
Department of Dermatology, Warsaw Medical School, Poland.
Jóźwiak
J
J
Osiecka
A
A
Roszkowski
P I
PI
Mazurkiewicz-Smoktunowicz
W
W
Rogoziński
T T
TT
Walczak
L
L
Jabłońska
S
S
Majewski
S
S
Malejczyk
J
J
D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D001913:Bowen's Disease / Q000097:blood*; D002294:Carcinoma, Squamous Cell / Q000097:blood*; D003218:Condylomata Acuminata / Q000097:blood*; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D027383:Papillomaviridae; D030361:Papillomavirus Infections / Q000097:blood*; D018124:Receptors, Tumor Necrosis Factor / Q000097:blood*; D014412:Tumor Virus Infections / Q000097:blood*; D002583:Uterine Cervical Neoplasms / Q000097:blood*; D002578:Uterine Cervical Dysplasia / Q000097:blood*
D018124:Receptors, Tumor Necrosis Factor