title: Endothelial ectoenzyme assays estimate perfused capillary surface area in the dog lung.
abstract: Whether the pulmonary vascular bed accommodates flow-induced increases in blood volume mainly through recruitment of previously unperfused capillaries or distension of already perfused vessels remains controversial. The modified first order reaction parameter of an enzyme and substrate, Amax/K(m), is, under nontoxic conditions, proportional to enzyme mass. Thus for ACE, an endothelium-bound ectoenzyme uniformly distributed along the luminal surface of the pulmonary capillary bed, Amax/K(m) is proportional to the dynamically perfused capillary surface area (PCSA). We estimated single-pass translobar hydrolysis and calculated the corresponding Amax/K(m) values of the synthetic ACE substrate 3H-benzoyl-Phe-Ala-Pro (BPAP), under first-order reaction conditions, in isolated blood-perfused dog lung lobes. We additionally studied blood flow distribution using radioactive microsphere techniques. Experiments were performed under zone III conditions over a wide range of lobar blood flow rates (Qb). As Qb was increased, Amax/K(m) rose linearly, while lobar vascular resistance (LVR) decreased, suggesting capillary recruitment rather than distension. Single pass BPAP hydrolysis (v approximately 2.9 at resting Qb) was not altered over a wide range of Qb, indicative of unchanging capillary transit times. When full capillary recruitment was achieved (at Qb > 70 ml/min/g lung wet weight), further Qb elevations failed to increase Amax/K(m), but decreased BPAP hydrolysis, denoting shorter transit times through the fully recruited capillary bed. Our data indicate that, as previously shown for rabbit lung, in this canine model, increases in pulmonary blood volume are mainly accommodated through recruitment of previously unperfused capillaries throughout the entire lung.
authors.affiliation: Department of Pharmacology, and Toxicology, Medical College of Georgia, Augusta 30912, USA.
authors.forename: S E
authors.initials: SE
authors.lastname: Orfanos
authors.forename: I C
authors.initials: IC
authors.lastname: Ehrhart
authors.forename: S
authors.initials: S
authors.lastname: Barman
authors.forename: W F
authors.initials: WF
authors.lastname: Hofman
authors.forename: J D
authors.initials: JD
authors.lastname: Catravas
mesh_terms: D000818:Animals; D002196:Capillaries / Q000201:enzymology / Q000648:ultrastructure; D004285:Dogs; D004730:Endothelium, Vascular / Q000201:enzymology*; D005260:Female; D006439:Hemodynamics / Q000502:physiology; D007700:Kinetics; D008297:Male; D008863:Microspheres; D007703:Peptidyl-Dipeptidase A / Q000032:analysis*; D011652:Pulmonary Circulation / Q000502:physiology*; D013499:Surface Properties
chemical_list: D007703:Peptidyl-Dipeptidase A