Diet-induced insulin resistance is associated with decreased activity of type I iodothyronine 5'-deiodinase in rat liver.
The effect of feeding Wistar rats with high-sucrose (63 wt% of sucrose, HS) or high-fat (30 wt% of fat, HF) diets for two weeks on serum selenium concentration and type I iodothyronine 5'-deiodinase (5'-DI) activity in liver was investigated. No significant differences in serum selenium concentration (as determined by graphite-furnace atomic absorption spectrometry) were found among the groups of rats fed basal, HS, or HF diets. A significant reduction of the 5'-DI activity (p < 0.005-0.05) was found in groups of rats fed either HS or HF diet in comparison with rats fed B diet. In conclusion, it is suggested that decreased 5'-DI activity in HS or HF diet-induced insulin resistance is not due to selenium status, but it may involve other dietary-related factors.
Bransová
J
J
Institute of Experimental Endocrinology, Slovak Academy of Sciences, Bratislava, Slovak Republic.
Brtková
A
A
Seböková
E
E
Klimes
I
I
Langer
P
P
Brtko
J
J
D000818:Animals; D004032:Diet; D004041:Dietary Fats / Q000008:administration & dosage; D007333:Insulin Resistance; D007453:Iodide Peroxidase / Q000378:metabolism*; D008099:Liver / Q000201:enzymology*; D008297:Male; D051381:Rats; D017208:Rats, Wistar; D013395:Sucrose / Q000008:administration & dosage
D004041:Dietary Fats; D013395:Sucrose; D007453:Iodide Peroxidase