title: Plasma-modified nylon meshes as supports for cell culturing.
abstract: The polymeric surfaces of three commercially available nylon films with mesh openings of 5 microns, 10 microns and 20 microns were treated with anhydrous ammonia gaseous plasma. Cells cultured on the plasma-treated nylon films have higher proliferation rate and assume morphology distinct from those cultured on the unmodified films. Of the three plasma-modified membranes, the one with 5 microns mesh openings supported a largest population of cell growth. The plasma-treated nylon meshes provided a stronger anchorage for the collagen matrices formed within the mesh openings. Application of this collagen/nylon meshes for cell culturing is demonstrated.
authors.affiliation: Institute of Biomedical Engineering, National Yang Ming University, Shi Pai, Pei Tou, Taipei, Taiwan, ROC.
authors.forename: S M
authors.initials: SM
authors.lastname: Kuo
authors.forename: S W
authors.initials: SW
authors.lastname: Tsai
authors.forename: L H
authors.initials: LH
authors.lastname: Huang
authors.forename: Y J
authors.initials: YJ
authors.lastname: Wang
mesh_terms: D016475:3T3 Cells; D000641:Ammonia; D000812:Anhydrides; D000818:Animals; D018929:Cell Culture Techniques / Q000379:methods*; D003094:Collagen; D008567:Membranes, Artificial; D051379:Mice; D008855:Microscopy, Electron, Scanning; D009757:Nylons / Q000378:metabolism; D010949:Plasma
chemical_list: D000812:Anhydrides; D008567:Membranes, Artificial; D009757:Nylons; D000641:Ammonia; D003094:Collagen