Amplification of avian reovirus RNA using the reverse transcriptase-polymerase chain reaction.
A reverse transcriptase-polymerase chain reaction method was developed for the detection of avian reovirus. The origin of primers was from the S1 gene of the avian reovirus genome. A reovirus-specific 532-base pair cDNA product was amplified by these primers from six reference strains and 23 field isolates of avian reoviruses, but not from seven different avian pathogenic viruses and bacteria. As little as 1 pg of avian reovirus RNA was detected using gel electrophoresis and Southern blot hybridization.
Xie
Z
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Department of Pathobiology, College of Agriculture and Natural Resources, University of Connecticut, Storrs 06269-3089, USA.
Fadl
A A
AA
Girshick
T
T
Khan
M I
MI
D000818:Animals; D002642:Chick Embryo; D002645:Chickens; D017931:DNA Primers; D005347:Fibroblasts; D018165:Orthoreovirus / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D016133:Polymerase Chain Reaction / Q000379:methods*; D011201:Poultry Diseases; D012194:RNA-Directed DNA Polymerase; D012088:Reoviridae Infections / Q000175:diagnosis / Q000662:veterinary*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
D017931:DNA Primers; D012194:RNA-Directed DNA Polymerase