[Biologic effect of LDL binding and intracellular degradation in monocytes from patients with hypercholesterolemia].
The granulocytes from elderly patients were investigated, in previous studies, with FMLP and it was found that the postreceptor signal, the inositol phosphate production and inositol phosphate dependent calcium signal were markedly reduced. It was observed that the 125I LDL binding was slightly reduced while the intracellular degradation of the LDL and endogenous cholesterol synthesis inhibitory effect was significantly decreased on monocytes of patients with non insulin dependent diabetes mellitus. It was suggested that of in patients suffering from NIDDM with hypercholesterolemia the LDL receptor numbers of monocytes are close to normal, while the post receptor signal transmission is damaged. In this study the monocytes from 12 patients with hypercholesterolemia were investigated before and after LDL treatment and were compared to the 11 age-matched healthy volunteer control patients. The cells were stimulated with LDL and chemotactic peptide FMLP. The postreceptor signal mechanism in monocytes was investigated. According to the results the inositol phosphate level of the patient group decreased independently from the stimulus. The LDL induced IP3 and Ca2+ level elevation was PT resistant both in the control and in the patients group.
Fóris
G
G
Debreceni Orvostudományi Egyetem, Központi Kutató Laboratórium.
Kovács
E
E
Szabolcs
M
M
Varga
Z
Z
Keresztes
T
T
Paragh
G
G
D000368:Aged; D002784:Cholesterol / Q000096:biosynthesis; D005260:Female; D006801:Humans; D006937:Hypercholesterolemia / Q000378:metabolism*; D007294:Inositol / Q000378:metabolism; D008077:Lipoproteins, LDL / Q000378:metabolism*; D008297:Male; D008875:Middle Aged; D009000:Monocytes / Q000378:metabolism*
D008077:Lipoproteins, LDL; D007294:Inositol; D002784:Cholesterol