title: Survival of Escherichia coli cells exposed to iodoacetate and chlorodinitrobenzene is independent of the glutathione-gated K+ efflux systems KefB and KefC.
abstract: The KefB and KefC systems of Escherichia coli cells are activated by iodoacetate (IOA) and chlorodinitrobenzene (CDNB), leading to a rapid drop in the intracellular pH. However, survival of exposure to IOA or CDNB was found to be essentially independent of KefB and KefC activation. No correlation was found between the toxicity of the compound and its ability to elicit protective acidification via activation of KefB and KefC.
authors.affiliation: Department of Molecular and Cell Biology, University of Aberdeen, Foresterhill, United Kingdom.
authors.forename: L S
authors.initials: LS
authors.lastname: Ness
authors.forename: G P
authors.initials: GP
authors.lastname: Ferguson
authors.forename: Y
authors.initials: Y
authors.lastname: Nikolaev
authors.forename: I R
authors.initials: IR
authors.lastname: Booth
mesh_terms: D017920:Antiporters / Q000378:metabolism*; D001426:Bacterial Proteins / Q000378:metabolism*; D002455:Cell Division / Q000187:drug effects; D004137:Dinitrochlorobenzene / Q000494:pharmacology* / Q000633:toxicity; D004926:Escherichia coli / Q000187:drug effects* / Q000254:growth & development / Q000378:metabolism*; D029968:Escherichia coli Proteins; D005978:Glutathione / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D007461:Iodoacetates / Q000494:pharmacology* / Q000633:toxicity; D019807:Iodoacetic Acid; D015640:Ion Channel Gating; D017136:Ion Transport / Q000187:drug effects; D015221:Potassium Channels / Q000378:metabolism*; D027961:Potassium-Hydrogen Antiporters
chemical_list: D017920:Antiporters; D001426:Bacterial Proteins; D004137:Dinitrochlorobenzene; D029968:Escherichia coli Proteins; D007461:Iodoacetates; D015221:Potassium Channels; D027961:Potassium-Hydrogen Antiporters; C069287:KefC protein, E coli; D005978:Glutathione; D019807:Iodoacetic Acid