Matrilin-3 from chicken cartilage.
By subtractive cDNA cloning we have identified a novel constituent of chicken cartilage termed matrilin-3. This constituent is encoded by a mRNA of 2.2 kbp whose expression is restricted to cartilaginous tissues. The predicted protein is composed of 452 amino acids with a molecular mass of 49 kDa. It contains a single von Willebrand factor A-like domain, four epidermal growth factor-like repeats and an alpha-helical region which may induce the formation of oligomers via a coiled-coil. The primary structure is similar to that of matrilin-1 which is also expressed in a cartilage-specific manner. This similarity suggests that the genes for the two proteins may have evolved from a common ancestor by gene duplication.
Belluoccio
D
D
MEM Institute, Division of Biology, University of Bern, Switzerland.
Trueb
B
B
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D015152:Blotting, Northern; D002356:Cartilage / Q000737:chemistry* / Q000166:cytology; D002642:Chick Embryo; D003001:Cloning, Molecular; D016326:Extracellular Matrix Proteins / Q000032:analysis / Q000737:chemistry* / Q000235:genetics; D006801:Humans; D017403:In Situ Hybridization; D064235:Matrilin Proteins; D008969:Molecular Sequence Data; D012333:RNA, Messenger / Q000032:analysis; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA
D016326:Extracellular Matrix Proteins; D064235:Matrilin Proteins; D012333:RNA, Messenger