title: Chondrocyte transplantation using a collagen bilayer matrix for cartilage repair.
abstract: We have developed a novel, two-layered, collagen matrix seeded with chondrocytes for repair of articular cartilage. It consists of a dense collagen layer which is in contact with bone and a porous matrix to support the seeded chondrocytes. The matrices were implanted in rabbit femoral trochleas for up to 24 weeks. The control groups received either a matrix without cells or no implant. The best histological repair was seen with cell-seeded implants. The permeability and glycosaminoglycan content of both implant groups were nearly normal, but were significantly less in tissue from empty defects. The type-II collagen content of the seeded implants was normal. For unseeded implants it was 74.3% of the normal and for empty defects only 20%. The current treatments for articular injury often result in a fibrous repair which deteriorates with time. This bilayer implant allowed sustained hyaline-like repair of articular defects during the entire six-month period of observation.
authors.affiliation: Hospital for Joint Diseases, New York, NY 10003, USA.
authors.forename: S R
authors.initials: SR
authors.lastname: Frenkel
authors.forename: B
authors.initials: B
authors.lastname: Toolan
authors.forename: D
authors.initials: D
authors.lastname: Menche
authors.forename: M I
authors.initials: MI
authors.lastname: Pitman
authors.forename: J M
authors.initials: JM
authors.lastname: Pachence
mesh_terms: D000818:Animals; D002358:Cartilage, Articular / Q000033:anatomy & histology / Q000293:injuries* / Q000601:surgery*; D019902:Chondrocytes / Q000637:transplantation*; D003094:Collagen / Q000032:analysis / Q000145:classification / Q000502:physiology*; D004195:Disease Models, Animal; D006025:Glycosaminoglycans / Q000032:analysis; D007150:Immunohistochemistry; D008297:Male; D010539:Permeability; D011817:Rabbits; D013997:Time Factors
chemical_list: D006025:Glycosaminoglycans; D003094:Collagen