Hormone-regulatable neoplastic transformation induced by a Jun-estrogen receptor chimera.
The v-jun oncogene encodes a nuclear DNA binding protein that functions as a transcription factor and is part of the activator protein 1 complex. Oncogenic transformation by v-jun is thought to be mediated by the aberrant expression of specific target genes. To identify such Jun-regulated genes and to explore the mechanisms by which Jun affects their expression, we have fused the full-length v-Jun and an amino-terminally truncated form of v-Jun to the hormone-binding domain of the human estrogen receptor. The two chimeric proteins function as ligand-inducible transactivators. Expression of the fusion proteins in chicken embryo fibroblasts causes estrogen-dependent transformation.
Kruse
U
U
The Scripps Research Institute, Department of Molecular and Experimental Medicine, BCC239, 10550 North Torrey Pines Road, La Jolla, CA 92037, USA.
Iacovoni
J S
JS
Goller
M E
ME
Vogt
P K
PK
D000818:Animals; D002471:Cell Transformation, Neoplastic / Q000235:genetics*; D002478:Cells, Cultured; D002642:Chick Embryo; D004967:Estrogens / Q000494:pharmacology*; D005347:Fibroblasts / Q000473:pathology; D015972:Gene Expression Regulation, Neoplastic / Q000187:drug effects; D018390:Gene Targeting; D016758:Genes, jun; D006801:Humans; D011960:Receptors, Estrogen / Q000235:genetics* / Q000378:metabolism; D011993:Recombinant Fusion Proteins / Q000235:genetics*
D004967:Estrogens; D011960:Receptors, Estrogen; D011993:Recombinant Fusion Proteins