Expression of leptin receptor mRNA (long form splice variant) in the human cerebellum.
Primers specific to the long isoform of leptin receptor (OB-Rb) mRNA were used in reverse transcriptase-linked polymerase chain reactions (RT-PCR) to investigate the expression of this receptor in the hypothalamus and cerebellum of human and rat. For both regions, we observed RT-PCR cDNAs as well as restriction enzyme cleavage fragments of expected sizes. Additionally, in situ hybridization of human cerebellum using two independent [35S]oligonucleotide probes complementary to the OB-Rb mRNA sequence revealed a prominent hybridization signal within the granular layer. Overall, our findings demonstrate the expression of OB-Rb mRNA in the cerebellum and suggest that in such a location, leptin receptors may mediate a function presumably not linked to body weight homeostasis.
Savioz
A
A
H.U.G., Belle-Idée, Division de Neuropsychiatrie, Geneva, Switzerland.
Charnay
Y
Y
Huguenin
C
C
Graviou
C
C
Greggio
B
B
Bouras
C
C
D000818:Animals; D002352:Carrier Proteins / Q000235:genetics*; D002531:Cerebellum / Q000378:metabolism*; D006801:Humans; D017403:In Situ Hybridization; D009765:Obesity; D012326:RNA Splicing; D012333:RNA, Messenger / Q000096:biosynthesis*; D051381:Rats; D011956:Receptors, Cell Surface; D018121:Receptors, Cytokine / Q000235:genetics*; D054411:Receptors, Leptin
D002352:Carrier Proteins; C515448:LEPR protein, human; D012333:RNA, Messenger; D011956:Receptors, Cell Surface; D018121:Receptors, Cytokine; D054411:Receptors, Leptin