title: The increased level of PDGF-A contributes to the increased proliferation induced by mechanical stimulation in osteoblastic cells.
abstract: Mechanical stimulation can prompt healing of bone fractures. However, it is largely unknown how osteogenesis is promoted by mechanical stimulation. In this study, we found that mechanical strain-induced proliferation of osteoblastic cells (MC3T3-E1) accompanied increased levels of platelet-derived growth factor-A (PDGF-A) mRNA, determined by quantitative reverse transcription/polymerase chain reaction. In addition, neomycin and W-7, which blocked mechanical strain-induced proliferation of the osteoblast cells, also blocked mechanical stimulation-induced elevation of PDGF-A mRNA. Finally, an antibody against PDGF can inhibit physical stimulation-induced proliferation of MC3T3-E1 cells, suggesting that the increased MC3T3-E1 cells produced by mechanical stimulation at least partially depends on the increased activity of PDGF.
authors.affiliation: Department of Orthopaedic Surgery, University of Pennsylvania School of Medicine, Philadelphia 19104-6081, USA.
authors.forename: W
authors.initials: W
authors.lastname: Wang
authors.forename: H
authors.initials: H
authors.lastname: Zhuang
authors.forename: C L
authors.initials: CL
authors.lastname: Levitz
authors.forename: H
authors.initials: H
authors.lastname: Fan
authors.forename: R M
authors.initials: RM
authors.lastname: Seldes
authors.forename: A D
authors.initials: AD
authors.lastname: Tahernia
authors.forename: C T
authors.initials: CT
authors.lastname: Brighton
mesh_terms: D002118:Calcium / Q000378:metabolism; D002147:Calmodulin / Q000378:metabolism; D002455:Cell Division; D002460:Cell Line; D007295:Inositol Phosphates / Q000378:metabolism; D010006:Osteoblasts / Q000166:cytology*; D010982:Platelet-Derived Growth Factor / Q000235:genetics / Q000378:metabolism*; D012333:RNA, Messenger / Q000378:metabolism; D013314:Stress, Mechanical
chemical_list: D002147:Calmodulin; D007295:Inositol Phosphates; D010982:Platelet-Derived Growth Factor; D012333:RNA, Messenger; D002118:Calcium