Chaperone-like function of lipocortin 1.
Lipocortin 1 (LC1) is a 37 kDa member of the annexin family of proteins. It has been proposed to act as a mediator of some of the actions of glucocorticoids in anti-inflammatory and immune suppressive functions. LC1 has been shown to play a role in cell proliferation, apoptosis, and differentiation. However, the exact biological functions of LC1 still remain obscure. Here it is shown that LC1 displays a chaperone-like function. Stoichiometric amounts of LC1 suppressed thermally induced inactivation and aggregation of the test enzymes citrate synthase and glutamate dehydrogenase. LC1 was also effective in refolding guanine hydrochloride-denatured glutamate dehydrogenase, as judged by circular dichroism spectroscopy.
Kim
G Y
GY
Department of Biochemistry, College of Medicine, University of Ulsan, Seoul, Korea.
Lee
H B
HB
Lee
S O
SO
Rhee
H J
HJ
Na
D S
DS
D000818:Animals; D017305:Annexin A1 / Q000494:pharmacology* / Q000502:physiology; D002417:Cattle; D002942:Circular Dichroism; D002950:Citrate (si)-Synthase / Q000187:drug effects; D005969:Glutamate Dehydrogenase / Q000187:drug effects; D006360:Heat-Shock Proteins / Q000187:drug effects; D006801:Humans; D018832:Molecular Chaperones / Q000494:pharmacology* / Q000502:physiology; D011487:Protein Conformation; D017510:Protein Folding; D013552:Swine; D013696:Temperature
D017305:Annexin A1; D006360:Heat-Shock Proteins; D018832:Molecular Chaperones; D005969:Glutamate Dehydrogenase; D002950:Citrate (si)-Synthase