title: Low and very low density lipoprotein composition and resistance to copper-induced oxidation are not notably modified in smokers.
abstract: To study whether tobacco use was associated with oxidative phenomena affecting lipoproteins, we estimated susceptibility of LDL and VLDL to an in vitro copper-mediated oxidation, and measured serum autoantibody titers against oxidized LDL in 45 middle-age healthy nonsmokers, 35 smokers and 37 ex-smokers of both sexes, taking into account the detailed lipid composition of the lipoproteins. VLDL from female smokers had higher triglyceride, phospholipid, apolipoprotein E and alpha-tocopherol content and showed a higher rate of copper-induced oxidation in comparison with those from nonsmokers (P < or = 0.05) whereas the relative composition of these particles in saturated, mono- or poly-unsaturated fatty acids was not modified by tobacco consumption. After adjustment for triglyceride content, no statistically significant difference in oxidation rate was observed. Lipid, alpha-tocopherol and protein composition of LDL did not appear to be influenced by smoking; in accordance with these observations, no difference in indices of in vitro oxidizability of LDL was noticed between the different groups. Autoantibody titers against oxLDL were similar in smokers and nonsmokers. We conclude that, in supposed healthy individuals, smoking does not seem to be associated with notable variations in composition of VLDL and LDL or with an increase of oxidizability of these atherogenic lipoproteins.
authors.affiliation: Centre de Médecine Préventive, Vandoeuvre-les-Nancy, France.
authors.forename: K
authors.initials: K
authors.lastname: Marangon
authors.forename: B
authors.initials: B
authors.lastname: Herbeth
authors.forename: Y
authors.initials: Y
authors.lastname: Artur
authors.forename: H
authors.initials: H
authors.lastname: Esterbauer
authors.forename: G
authors.initials: G
authors.lastname: Siest
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D001323:Autoantibodies / Q000097:blood; D003300:Copper / Q000494:pharmacology*; D005260:Female; D006801:Humans; D008055:Lipids / Q000032:analysis; D008077:Lipoproteins, LDL / Q000032:analysis / Q000097:blood* / Q000276:immunology; D008079:Lipoproteins, VLDL / Q000032:analysis / Q000097:blood*; D008297:Male; D008875:Middle Aged; D010084:Oxidation-Reduction / Q000187:drug effects; D011506:Proteins / Q000032:analysis; D012907:Smoking / Q000097:blood*; D014810:Vitamin E / Q000032:analysis
chemical_list: D001323:Autoantibodies; D008055:Lipids; D008077:Lipoproteins, LDL; D008079:Lipoproteins, VLDL; D011506:Proteins; D014810:Vitamin E; D003300:Copper