Construction of a semisynthetic antibody library using trinucleotide oligos.
A semisynthetic antibody library composed of single chain Fv fragments (scFv) was constructed by replacing the heavy chain CDR3 region of a human scFv by a random sequence of eight amino acids using trinucleotide codons. After cloning into a phage display vector, an antibody library was generated with a complexity of 8 x 10(8) independent clones. The library was screened for binders to dinitrophenol, fluorescein isothiocyanate and 3-nitro-4-hydroxy-5-iodophenylacetic acid. scFv antibodies that specifically bound the antigen were obtained in each case.
Braunagel
M
M
Recombinant Antibody Group, Division of Diagnosis and Experimental Therapy, Deutsches Krebsforschungszentrum, INF 280, 69120 Heidelberg, Germany. affitech@online.no
Little
M
M
D000906:Antibodies / Q000737:chemistry / Q000235:genetics* / Q000276:immunology; D000918:Antibody Specificity; D003001:Cloning, Molecular; D006801:Humans; D007128:Immunoglobulin Fragments / Q000737:chemistry / Q000235:genetics; D007135:Immunoglobulin Variable Region / Q000737:chemistry / Q000235:genetics; D009842:Oligopeptides / Q000737:chemistry / Q000235:genetics; D019151:Peptide Library
D000906:Antibodies; D007128:Immunoglobulin Fragments; D007135:Immunoglobulin Variable Region; D009842:Oligopeptides; D019151:Peptide Library; C055966:immunoglobulin Fv