title: Use of hematopoietic cytokines to accelerate the recovery of the immune system in irradiated mice.
abstract: In our previous studies aimed at designing appropriate strategies to accelerate recovery of the immune system after irradiation, we found that the hematopoietic cytokine recombinant murine (rmu) interleukin (IL)-3 was able to induce differentiation and growth of thymocytes and splenic T and B lymphocytes in mice exposed to x-rays (200-500 cGy). The recovery, however, was complete at 7 days only after a dose of 200 cGy, whereas 2, 3, and 4 weeks were necessary to achieve full recovery after 300, 400, and 500 cGy, respectively. These studies were extended to investigate the effects of another hematopoietic cytokine, recombinant human (rhu) IL-11, a bone marrow stromal-derived cytokine, administered together with IL-3 to irradiated mice. The synergistic effect of the two cytokines was evident when relatively small doses of rhu IL-11 were injected with an optimal dose of rmu IL-3.
authors.affiliation: Laboratory of Immunology, AMB-PRO-TOSS, ENEA CR Casaccia, Rome, Italy.
authors.forename: D
authors.initials: D
authors.lastname: Frasca
authors.forename: F
authors.initials: F
authors.lastname: Guidi
authors.forename: M
authors.initials: M
authors.lastname: Arbitrio
authors.forename: C
authors.initials: C
authors.lastname: Pioli
authors.forename: G
authors.initials: G
authors.lastname: Leter
authors.forename: M
authors.initials: M
authors.lastname: Spano
authors.forename: G
authors.initials: G
authors.lastname: Doria
mesh_terms: D000818:Animals; D001402:B-Lymphocytes / Q000187:drug effects / Q000276:immunology / Q000528:radiation effects*; D001854:Bone Marrow Cells / Q000187:drug effects / Q000528:radiation effects; D015496:CD4-Positive T-Lymphocytes / Q000528:radiation effects; D018414:CD8-Positive T-Lymphocytes / Q000528:radiation effects; D002453:Cell Cycle / Q000187:drug effects / Q000528:radiation effects; D016207:Cytokines / Q000494:pharmacology; D004307:Dose-Response Relationship, Radiation; D006410:Hematopoiesis / Q000528:radiation effects; D006801:Humans; D017370:Interleukin-11 / Q000494:pharmacology*; D007377:Interleukin-3 / Q000494:pharmacology*; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D008940:Mitotic Index / Q000187:drug effects / Q000528:radiation effects; D011994:Recombinant Proteins / Q000494:pharmacology; D013154:Spleen / Q000187:drug effects / Q000276:immunology / Q000528:radiation effects; D013601:T-Lymphocytes / Q000187:drug effects / Q000276:immunology / Q000528:radiation effects*; D013950:Thymus Gland / Q000187:drug effects / Q000276:immunology / Q000528:radiation effects; D014916:Whole-Body Irradiation; D014965:X-Rays
chemical_list: D016207:Cytokines; D017370:Interleukin-11; D007377:Interleukin-3; D011994:Recombinant Proteins