Reduced lipid peroxidation and ischemia-reperfusion injury after lung transplantation using low-potassium dextran solution for lung preservation.
Ischemia-reperfusion injury is one of the significant problems in clinical lung transplantation. We investigated the effect of lung preservation with Euro-Collins solution (EC group) or low-potassium dextran solution (LPD group) on lipid peroxidation and ischemia-reperfusion injury in a pig model of lung allotransplantation. The donor lungs were preserved at 4 degrees C for 18 h. Left-sided single lung transplantation was performed, followed by 6 h of reperfusion. Lipid peroxidation was measured as thiobarbituric acid-reactive materials (TBARM) in bronchoalveolar lavage (BAL) fluid and effluent solutions from pulmonary artery (Effluent). After 18 h of ischemia, the LPD group showed lower TBARM in BAL and Effluent than the EC group (p < 0.05). After ischemia plus reperfusion, lung wetto-dry weight ratios and TBARM levels in BAL in the LPD group were lower than those of the EC group (p < 0.05). Lung wet-to-dry weight ratios correlated with TBARM levels in BAL (p < 0.05, r = 0.50). We conclude lipid peroxidation in BAL and Effluent may reflect the degree of ischemia-reperfusion injury, and lung preservation with LPD can reduce lipid peroxidation and lung injury as compared with EC.
Sakamaki
F
F
Department of Surgery and Institute for Surgical Research, Klinikum Grosshadern, Ludwig-Maximilians-Universität München, Munich, Germany.
Hoffmann
H
H
Müller
C
C
Dienemann
H
H
Messmer
K
K
Schildberg
F W
FW
D000818:Animals; D001992:Bronchoalveolar Lavage Fluid / Q000737:chemistry; D003911:Dextrans / Q000494:pharmacology; D005947:Glucose / Q000494:pharmacology; D006439:Hemodynamics; D006982:Hypertonic Solutions / Q000494:pharmacology; D007958:Leukocyte Count; D015227:Lipid Peroxidation / Q000187:drug effects* / Q000502:physiology; D016040:Lung Transplantation / Q000009:adverse effects*; D009504:Neutrophils / Q000473:pathology; D009926:Organ Preservation / Q000379:methods*; D019188:Organ Preservation Solutions / Q000494:pharmacology*; D011188:Potassium; D015427:Reperfusion Injury / Q000209:etiology / Q000503:physiopathology / Q000517:prevention & control*; D013552:Swine; D017392:Thiobarbituric Acid Reactive Substances / Q000032:analysis
D003911:Dextrans; C037096:Euro-Collins' solution; D006982:Hypertonic Solutions; D019188:Organ Preservation Solutions; D017392:Thiobarbituric Acid Reactive Substances; C083290:low potassium dextran glucose solution; D005947:Glucose; D011188:Potassium