title: Multicenter typing comparison of sporadic and outbreak Clostridium difficile isolates from geographically diverse hospitals.
abstract: In a collaborative study by three laboratories, arbitrarily primed polymerase chain reaction (AP-PCR), HindIII restriction enzyme analysis (REA), and pulsed-field gel electrophoresis (PFGE) using SmaI were compared for typing of Clostridium difficile. The study included 30 isolates from nosocomial outbreaks in six geographically disparate hospitals and 15 isolates from sporadic cases of C. difficile diarrhea. REA distinguished a total of 23 types representing 10 groups; AP-PCR performed at Deaconess Hospital resolved 19 types; AP-PCR performed at the Centers for Disease Control resolved 15 types. Thirty isolates exhibited degradation of larger sized fragments during processing and therefore were nontypeable by PFGE; among the remaining 15 isolates, PFGE resolved 11 types. Outbreak isolates in five different hospitals represented REA group J and constituted a single AP-PCR strain. In summary, nosocomial outbreaks of C. difficile diarrhea in five hospitals were associated with a single genetic lineage as resolved by multiple strain typing systems.
authors.affiliation: Department of Medicine, Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, Massachusetts 02215, USA.
authors.forename: M
authors.initials: M
authors.lastname: Samore
authors.forename: G
authors.initials: G
authors.lastname: Killgore
authors.forename: S
authors.initials: S
authors.lastname: Johnson
authors.forename: R
authors.initials: R
authors.lastname: Goodman
authors.forename: J
authors.initials: J
authors.lastname: Shim
authors.forename: L
authors.initials: L
authors.lastname: Venkataraman
authors.forename: S
authors.initials: S
authors.lastname: Sambol
authors.forename: P
authors.initials: P
authors.lastname: DeGirolami
authors.forename: F
authors.initials: F
authors.lastname: Tenover
authors.forename: R
authors.initials: R
authors.lastname: Arbeit
authors.forename: D
authors.initials: D
authors.lastname: Gerding
mesh_terms: D015373:Bacterial Typing Techniques; D016360:Clostridioides difficile / Q000145:classification*; D003428:Cross Infection / Q000453:epidemiology / Q000382:microbiology*; D003967:Diarrhea / Q000453:epidemiology / Q000382:microbiology; D004196:Disease Outbreaks; D006801:Humans; D000091402:Prohibitins