title: Antibacterial activity of quinolones against coagulase-negative staphylococci and the quinolone resistance-determining region of the gyrA genes from six species.
abstract: Antibacterial activity of quinolones against three species of coagulase-negative staphylococci was investigated. Tosufloxacin and sparfloxacin exhibited potent activities against Staphylococcus epidermidis, Staphylococcus haemolyticus and Staphylococcus saprophyticus compared with other quinolones tested. From the analysis of the DNA sequence in the quinolone resistance-determining region (QRDR), greater than 80% homology was recognized in coagulase-negative staphylococci. A series residue was conserved in all six species at the position corresponding to position 84 in Staphylococcus aureus.
authors.affiliation: Research Laboratories, Toyama Chemical Co., Ltd, Japan.
authors.forename: M
authors.initials: M
authors.lastname: Takahata
authors.forename: M
authors.initials: M
authors.lastname: Yonezawa
authors.forename: N
authors.initials: N
authors.lastname: Matsubara
authors.forename: Y
authors.initials: Y
authors.lastname: Watanabe
authors.forename: H
authors.initials: H
authors.lastname: Narita
authors.forename: T
authors.initials: T
authors.lastname: Matsunaga
authors.forename: H
authors.initials: H
authors.lastname: Igarashi
authors.forename: M
authors.initials: M
authors.lastname: Kawahara
authors.forename: S
authors.initials: S
authors.lastname: Onodera
authors.forename: Y
authors.initials: Y
authors.lastname: Oishi
mesh_terms: D000890:Anti-Infective Agents / Q000494:pharmacology*; D001483:Base Sequence; D003030:Coagulase; D004269:DNA, Bacterial; D004352:Drug Resistance, Microbial / Q000235:genetics; D024841:Fluoroquinolones; D005798:Genes, Bacterial / Q000187:drug effects; D008826:Microbial Sensitivity Tests; D008969:Molecular Sequence Data; D009287:Naphthyridines / Q000494:pharmacology; D016133:Polymerase Chain Reaction; D015363:Quinolones / Q000494:pharmacology; D017422:Sequence Analysis, DNA; D012689:Sequence Homology, Nucleic Acid; D013210:Staphylococcus / Q000187:drug effects / Q000201:enzymology / Q000235:genetics*; D013212:Staphylococcus epidermidis / Q000187:drug effects / Q000201:enzymology / Q000235:genetics
chemical_list: D000890:Anti-Infective Agents; D003030:Coagulase; D004269:DNA, Bacterial; D024841:Fluoroquinolones; D009287:Naphthyridines; D015363:Quinolones; C055185:tosufloxacin; C061363:sparfloxacin