Solution NMR spectroscopy beyond 25 kDa.
Improvements in NMR instrumentation, higher magnetic field strengths, novel NMR experiments and new deuterium-labeling strategies have significantly increased the scope of structural problems that can now be addressed by solution NMR methods. To date, a number of structures of proteins of 30 kDa have been solved using multidimensional 15N,13C,2H NMR techniques, and this molecular weight limit will probably be surpassed in the near future.
Kay
L E
LE
Department of Medical Genetics, University of Toronto, Ontario, Canada. kay@bloch.med.utoronto.ca
Gardner
K H
KH
D001665:Binding Sites; D002247:Carbon Isotopes; D004247:DNA / Q000737:chemistry*; D003903:Deuterium; D008024:Ligands; D009682:Magnetic Resonance Spectroscopy / Q000379:methods; D008970:Molecular Weight; D009587:Nitrogen Isotopes; D011506:Proteins / Q000737:chemistry* / Q000378:metabolism; D012313:RNA / Q000737:chemistry*
D002247:Carbon Isotopes; D008024:Ligands; D009587:Nitrogen Isotopes; D011506:Proteins; D012313:RNA; D004247:DNA; D003903:Deuterium