title: Effects of various concentrations of native seminal plasma in cryoprotectant on viability of human sperm.
abstract: To investigate the effect of native seminal plasma on the recovery of frozen human sperm, various concentrations of seminal plasma (0, 25, 50, 75, and 100%) were used in cryoprotectant for freezing sperm, and the viabilities of frozen-thawed sperm were compared. The post-thaw sperm motility of 50 or 75% seminal plasma in the fertile group was significantly higher than that of 0, 25, or 100%. The post-thaw motility of 75% donor seminal plasma in the patient group was higher than that of other concentrations. It was suggested that a certain concentration of native seminal plasma in cryoprotectant would be helpful to the viability of human sperm cryopreservation.
authors.affiliation: Institute of Guangzhou Family Planning, China.
authors.forename: W X
authors.initials: WX
authors.lastname: Ben
authors.forename: M T
authors.initials: MT
authors.lastname: Fu
authors.forename: L K
authors.initials: LK
authors.lastname: Mao
authors.forename: Z W
authors.initials: ZW
authors.lastname: Ming
authors.forename: W W
authors.initials: WW
authors.lastname: Xiong
mesh_terms: D002470:Cell Survival; D015925:Cryopreservation; D003451:Cryoprotective Agents / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D006801:Humans; D008297:Male; D012661:Semen / Q000502:physiology*; D012662:Semen Preservation; D013081:Sperm Motility / Q000187:drug effects; D013094:Spermatozoa / Q000187:drug effects* / Q000502:physiology
chemical_list: D003451:Cryoprotective Agents