Crystallization and preliminary crystallographic analysis of the sarcosine oxidase from Bacillus sp. NS-129.
The sarcosine oxidase from Bacillus sp. NS-129 has been crystallized by vapor diffusion using ammonium sulfate as precipitant. The tetragonal crystals have P4(1)2(1)2 (or P4(3)2(1)2) symmetry with a = b = 177.0 and c = 72.6 A. The crystals probably contain two sarcosine oxidase molecules per asymmetric unit and diffract to at least 2.7 A resolution.
Ichikawa
T
T
Biotechnology Research Center, University of Tokyo, Japan.
Sasaki
H
H
Koike
H
H
Nishiyama
M
M
Koyama
Y
Y
Tanokura
M
M
D000645:Ammonium Sulfate; D001407:Bacillus / Q000201:enzymology*; D003460:Crystallization; D018360:Crystallography, X-Ray / Q000379:methods; D010089:Oxidoreductases, N-Demethylating / Q000737:chemistry* / Q000302:isolation & purification; D050879:Sarcosine Oxidase
D010089:Oxidoreductases, N-Demethylating; D050879:Sarcosine Oxidase; D000645:Ammonium Sulfate