title: A comparison of functional and structural consequences of the tyrosine B10 and glutamine E7 motifs in two invertebrate hemoglobins (Ascaris suum and Lucina pectinata).
abstract: The architecture of the distal heme pocket in hemoglobins and myoglobins can play an important role in controlling ligand binding dynamics. The size and polarity of the residues occupying the distal pocket may contribute steric and dielectric effects. In vertebrate systems, the distal pocket typically contains a "distal" histidine at position E7 and a leucine at position B10. There are several invertebrate organisms that have hemoglobins or myoglobins that display a pattern in which residues E7 and B10 are a glutamine and tyrosine, respectively. These proteins often have very high oxygen affinities stemming from very slow ligand off rates. In this study, two such hemoglobins, one from the nematode Ascaris suum and the other from the sulfide-fixing clam Lucina pectinata, are compared with respect to conformational and functional properties. Ultraviolet resonance Raman spectroscopy and visible resonance Raman spectroscopy are used to probe, respectively, the ligand-dependent hydrogen bonding pattern of the tyrosine residues and the proximal heme pocket interactions. Fourier transform infrared absorption spectroscopy is used to probe the dielectric properties of the distal heme pocket through the stretching frequency of carbon monoxide bound to the heme. Functionality is probed through the geminate rebinding of both CO and O2. The findings reveal two very different patterns indicative of two different mechanisms for achieving low oxygen off rates. In Hb Ascaris, a hydrogen bonding network that includes the E7 Gln, B10 Tyr, and oxygen bound to the heme results in a tight cage for the oxygen. Dissociation of the O2 requires a large amplitude conformational fluctuation that results both in a spontaneous dissociation of the oxygen through the loss of hydrogen bond stabilization and in an enhanced probability for ligand escape though the transient disruption and opening of the tight distal cage. In the case of the Hb from Lucina, there is no evidence for a tight cage. Instead the data support a model in which the hydrogen bonding network is far more tenuous and the equilibrium state of distal pocket is far more open and accessible than is the case in Ascaris. The results explain why Hb Ascaris has one of the highest oxygen affinities known (P50 approximately 10(-)3 Torr) while Hb Lucina II has an oxygen affinity comparable to that of Mb (P50 = 0.13 Torr) even though both of these Hbs contain the B10 Tyr and E7 Gln motif and display very low oxygen off rates. The roles of water and proximal strain are discussed.
authors.affiliation: Department of Physiology and Biophysics, Albert Einstein College of Medicine, Bronx, New York 10461, USA.
authors.forename: E S
authors.initials: ES
authors.lastname: Peterson
authors.forename: S
authors.initials: S
authors.lastname: Huang
authors.forename: J
authors.initials: J
authors.lastname: Wang
authors.forename: L M
authors.initials: LM
authors.lastname: Miller
authors.forename: G
authors.initials: G
authors.lastname: Vidugiris
authors.forename: A P
authors.initials: AP
authors.lastname: Kloek
authors.forename: D E
authors.initials: DE
authors.lastname: Goldberg
authors.forename: M R
authors.initials: MR
authors.lastname: Chance
authors.forename: J B
authors.initials: JB
authors.lastname: Wittenberg
authors.forename: J M
authors.initials: JM
authors.lastname: Friedman
mesh_terms: D019943:Amino Acid Substitution; D000818:Animals; D017165:Ascaris suum; D001665:Binding Sites; D049872:Bivalvia; D002248:Carbon Monoxide / Q000378:metabolism; D002263:Carboxyhemoglobin / Q000737:chemistry* / Q000378:metabolism; D018698:Glutamic Acid; D006418:Heme; D006454:Hemoglobins / Q000737:chemistry* / Q000378:metabolism*; D007700:Kinetics; D010108:Oxyhemoglobins / Q000737:chemistry* / Q000378:metabolism; D011487:Protein Conformation; D017550:Spectroscopy, Fourier Transform Infrared; D013059:Spectrum Analysis, Raman; D013816:Thermodynamics; D014443:Tyrosine
chemical_list: D006454:Hemoglobins; D010108:Oxyhemoglobins; D018698:Glutamic Acid; D014443:Tyrosine; D006418:Heme; D002248:Carbon Monoxide; D002263:Carboxyhemoglobin