Antibodies against dengue virus E protein peptide bind to human plasminogen and inhibit plasmin activity.
Both mice and rabbits immunized with dengue virus E protein peptide spanning amino acids 100-119 (D4E) produced antibodies that reacted not only with the D4E peptide itself but also with human plasminogen, as shown by ELISA and Western blot. Sera from dengue virus-hyperimmunized mice and dengue patients also contained antibodies against D4E and plasminogen. Furthermore, such sera all contained plasmin inhibitory activity. Using affinity-purified anti-D4E antibodies and free D4E peptide for competitive inhibition, we demonstrated that the inhibition of plasmin activity was due to anti-D4E antibodies rather than other substances in the sera. Taken together, these results suggest dengue virus E protein amino acids 100-119 are a cross-reactive immunogenic region, and antibodies against this region may interfere with human fibrinolysis.
Huang
Y H
YH
Department of Microbiology and Immunology, College of Medicine, National Cheng Kung University, Tainan, Taiwan, ROC.
Chang
B I
BI
Lei
H Y
HY
Liu
H S
HS
Liu
C C
CC
Wu
H L
HL
Yeh
T M
TM
D000818:Animals; D000914:Antibodies, Viral / Q000276:immunology*; D000956:Antigens, Viral / Q000276:immunology; D003716:Dengue Virus / Q000276:immunology*; D005260:Female; D005341:Fibrinolysin / Q000037:antagonists & inhibitors* / Q000276:immunology; D006801:Humans; D051379:Mice; D008807:Mice, Inbred BALB C; D010455:Peptides / Q000276:immunology; D010958:Plasminogen / Q000276:immunology*; D011817:Rabbits; D014759:Viral Envelope Proteins / Q000276:immunology*
D000914:Antibodies, Viral; D000956:Antigens, Viral; C051821:E-glycoprotein, Dengue virus type 2; D010455:Peptides; D014759:Viral Envelope Proteins; D010958:Plasminogen; D005341:Fibrinolysin