Contractile effects of porcine galanin(1-29)-NH2 on the rat isolated gastric fundus: mediation by potassium ions.
Galanin (3-300 nM) evoked reproducible concentration-dependent contractions of rat isolated gastric fundus strips, EC50 of the peptide equalled 16.7 nM (6.2-39.2) and the slope of the concentration-response curve was 34.8 (24.0-45.7). The maximal response (Emax) to carbachol (30 nM) was not affected by the absence of the potassium ions in the bathing solution. On the contrary the Emax to galanin (300 nM) was decreased by almost 95% by the use of the potassium-free buffer. Re-exposure of the muscle strips to potassium containing bathing medium reversed the inhibition by about 35%, yet the value remained significantly lower than that of the control. Apamin (1 and 2 microM), glybenclamide (10 microM), clofilium tosylate (10 microM) did not significantly influence the Emax to carbachol. Apamin or glybenclamide did not affect the contractile action of galanin, while clofilium attenuated the Emax to the peptide in a concentration-dependent manner, the EC50 of the agent being 9.44 microM (164 nM-541 microM). It was concluded that the potassium ions play a modulatory role in gastric smooth muscle contraction following galanin receptor stimulation, probably by interacting with the extracellular calcium influx.
Korolkiewicz
R
R
Department of Pharmacology, Medical University of Gdansk, Do Studzienki 38, Gdansk, 80-227, Poland.
Takeuchi
K
K
Sliwinski
W
W
Konstanski
Z
Z
Rekowski
P
P
Szyk
A
A
D000818:Animals; D002021:Buffers; D002217:Carbachol / Q000494:pharmacology; D005260:Female; D019004:Galanin / Q000494:pharmacology*; D005748:Gastric Fundus / Q000187:drug effects / Q000502:physiology; D066298:In Vitro Techniques; D008297:Male; D009119:Muscle Contraction / Q000187:drug effects; D009130:Muscle, Smooth / Q000187:drug effects*; D011188:Potassium / Q000494:pharmacology*; D026902:Potassium Channel Blockers; D000644:Quaternary Ammonium Compounds / Q000494:pharmacology; D051381:Rats; D017208:Rats, Wistar; D013552:Swine
D002021:Buffers; D026902:Potassium Channel Blockers; D000644:Quaternary Ammonium Compounds; C022799:clofilium; D019004:Galanin; D002217:Carbachol; D011188:Potassium