Increased sensitivity of HIV-1 antibody detection.
Clinical trial results from 11,344 paired urine and serum samples revealed 1,181 HIV-1-positive individuals confirmed by western blot (WB). There were 25 discrepant samples: 10 were urine enzyme immunoassay (EIA) and WB positive, serum non-reactive and serum WB negative or indeterminate, and 15 were serum EIA and WB positive, urine EIA non-reactive or urine WB negative or indeterminate. Serum samples, HIV-1 antibody WB confirmed, revealed a 99.15% sensitivity (1,171 out of 1,181); urine samples, HIV-1 antibody WB confirmed, showed a 98.73% sensitivity (1,166 out of 1,181). This study demonstrated that neither serum nor urine results alone are as sensitive for HIV-1 antibody detection as combined results of both samples.
Urnovitz
H B
HB
Calypte Biomedical, California 94710, USA. hervdoc@aol.com
Sturge
J C
JC
Gottfried
T D
TD
D015492:AIDS Serodiagnosis / Q000379:methods*; D015153:Blotting, Western; D005188:False Negative Reactions; D005189:False Positive Reactions; D015483:HIV Antibodies / Q000032:analysis*; D006679:HIV Seropositivity / Q000097:blood / Q000175:diagnosis / Q000652:urine; D015497:HIV-1 / Q000276:immunology*; D006801:Humans; D007124:Immunoenzyme Techniques; D012680:Sensitivity and Specificity
D015483:HIV Antibodies