Reassessment of FDG uptake in tumor cells: high FDG uptake as a reflection of oxygen-independent glycolysis dominant energy production.
To determine appropriate use of 2-[18F]-fluoro-2-deoxy-D-glucose (FDG) in the diagnosis of malignant tumors, the mechanism of enhanced FDG uptake in tumor cells was reassessed using in vitro cultured cell lines and 3H-deoxyglucose (DG), in combination with possible parameters of aerobic and anaerobic energy production. The high DG uptake in the tumor cells reflected the dependency of energy production on anaerobic glycolysis, and paradoxically on low levels of aerobic oxidative phosphorylation in mitochondria. We discuss here factors underlying anaerobic glycolysis in tumor cells.
Waki
A
A
Biomedical Imaging Research Center, Fukui Medical School, Japan.
Fujibayashi
Y
Y
Yonekura
Y
Y
Sadato
N
N
Ishii
Y
Y
Yokoyama
A
A
D000255:Adenosine Triphosphate / Q000502:physiology; D000693:Anaerobiosis / Q000502:physiology; D002455:Cell Division / Q000502:physiology; D002460:Cell Line; D004396:Coloring Agents; D004734:Energy Metabolism / Q000502:physiology*; D019788:Fluorodeoxyglucose F18 / Q000493:pharmacokinetics*; D006019:Glycolysis / Q000502:physiology*; D006593:Hexokinase / Q000378:metabolism; D006801:Humans; D019344:Lactic Acid / Q000378:metabolism; D008564:Membrane Potentials / Q000502:physiology; D008928:Mitochondria / Q000201:enzymology / Q000378:metabolism; D010078:Oxazines; D010085:Oxidative Phosphorylation; D010101:Oxygen Consumption / Q000502:physiology; D014966:Xanthenes
D004396:Coloring Agents; D010078:Oxazines; D014966:Xanthenes; D019788:Fluorodeoxyglucose F18; C005843:resazurin; D019344:Lactic Acid; D000255:Adenosine Triphosphate; D006593:Hexokinase