title: Multiple bacteriocin production by Leuconostoc mesenteroides TA33a and other Leuconostoc/Weissella strains.
abstract: Leuconostoc (Lc.) mesenteroides TA33a produced three bacteriocins with different inhibitory activity spectra. Bacteriocins were purified by adsorption/desorption from producer cells and reverse phase high-performance liquid chromatography. Leucocin C-TA33a, a novel bacteriocin with a predicted molecular mass of 4598 Da, inhibited Listeria and other lactic acid bacteria (LAB). Leucocin B-TA33a has a predicted molecular mass of 3466 Da, with activity against Leuconostoc/Weissella (W.) strains, and appears similar to mesenterocin 52B and dextranicin 24, while leucocin A-TA33a, which also inhibited Listeria and other LAB strains, is identical to leucocin A-UAL 187. A survey of other known bacteriocin-producing Leuconostoc/Weissella strains for the presence of the three different bacteriocins revealed that production of leucocin A-, B- and C-type bacteriocins was widespread. Lc. carnosum LA54a, W. paramesenteroides LA7a, and Lc. gelidum UAL 187-22 produced all three bacteriocins, whereas W. paramesenteroides OX and Lc. carnosum TA11a produced only leucocin A- and B-type bacteriocins.
authors.affiliation: Department of Microbiology, University of the Witwatersrand, South Africa.
authors.forename: M A
authors.initials: MA
authors.lastname: Papathanasopoulos
authors.forename: F
authors.initials: F
authors.lastname: Krier
authors.forename: A M
authors.initials: AM
authors.lastname: Revol-Junelles
authors.forename: G
authors.initials: G
authors.lastname: Lefebvre
authors.forename: J P
authors.initials: JP
authors.lastname: Le Caer
authors.forename: A
authors.initials: A
authors.lastname: von Holy
authors.forename: J W
authors.initials: JW
authors.lastname: Hastings
mesh_terms: D001419:Bacteria / Q000187:drug effects; D001430:Bacteriocins / Q000302:isolation & purification* / Q000378:metabolism / Q000494:pharmacology; D007934:Leuconostoc / Q000378:metabolism*
chemical_list: D001430:Bacteriocins