Homology-dependent silencing of the SC3 gene in Schizophyllum commune.
After introduction of extra copies of the SC3 hydrophobin gene into a wild-type strain of Schizophyllum commune, gene silencing was observed acting on both endogenous and introduced SC3 genes in primary vegetative transformants. Nuclear run-on experiments indicated that silencing acted at the transcriptional level. Southern analysis revealed that cytosine methylation of genomic DNA occurred. Moreover, SC3 silencing was suppressed by exposure to 5-azacytidine during growth. After growth of SC3-suppressed colonies from homogenized mycelium or from colonies stored at 4 degrees, SC3 transcription was restored. However, after prolonged growth SC3 silencing was again observed. Introduction of a promoterless SC3 fragment into wild type gave less SC3 silencing.
Schuurs
T A
TA
Department of Plant Biology, Groningen Biomolecular Sciences and Technology Institute, University of Groningen, Haren, The Netherlands.
Schaeffer
E A
EA
Wessels
J G
JG
D001374:Azacitidine / Q000494:pharmacology; D002467:Cell Nucleus / Q000378:metabolism; D019175:DNA Methylation; D005800:Genes, Fungal; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D012567:Schizophyllum / Q000235:genetics*; D014158:Transcription, Genetic; D014170:Transformation, Genetic
D012333:RNA, Messenger; D001374:Azacitidine