Estimation of the age of the ancestral arginine3500-->glutamine mutation in human apoB-100.
Familial defective apoB-100 (R3500Q) [FDB (R3500Q)] is caused by a mutation in the apoB gene (2p23.24). Almost all individuals with this disorder are of European descent, and in almost all cases the mutation is on a chromosome with a rare haplotype (194) at the apoB locus, suggesting that all FDB (R3500Q) probands are descended from a common ancestor in whom the original mutation occurred. The distribution of the mutation is consistent with an origin in Europe 6000-7000 years ago. We have estimated the amount of recombination between the apoB gene and markers on chromosome 2 in 34 FDB (R3500Q) probands in whom the mutation is on a 194 haplotype. Significant linkage disequilibrium was found between the apoB gene and marker D2S220. We have identified three YACs that contain the apoB gene and D2S220. The shortest restriction fragment common to the three YACs that contained both loci was 240 kb long. No shorter fragments with both loci were identified. On the assumption that 1000 kb corresponds to 1 cM, we deduce that the recombination distance between D2S220 and the apoB gene is about 0.24 cM. Combining this value with the linkage disequilibrium observed between the two loci in the probands, we estimate that the ancestral mutation occurred about 270 generations ago. We postulate that the original mutation occurred in the common ancestor of living FDB (R3500Q) probands, who lived in Europe about 6750 years ago. The errors in this estimate are discussed.
Myant
N B
NB
MRC Lipoprotein Team, Royal Postgraduate Medical School, Hammersmith Hospital, London.
Forbes
S A
SA
Day
I N
IN
Gallagher
J
J
D053299:Apolipoprotein B-100; D001055:Apolipoproteins B / Q000235:genetics*; D001120:Arginine / Q000235:genetics*; D018244:Chromosomes, Artificial, Yeast; D002889:Chromosomes, Human, Pair 2; D053938:DNA Fragmentation; D019143:Evolution, Molecular; D005260:Female; D005787:Gene Frequency; D005973:Glutamine / Q000235:genetics*; D006239:Haplotypes; D006801:Humans; D008297:Male; D017354:Point Mutation
D053299:Apolipoprotein B-100; D001055:Apolipoproteins B; D005973:Glutamine; D001120:Arginine