Effects of epidermal growth factor and insulin on migration and proliferation of primary cultured rabbit gastric epithelial cells.
We assessed the influence of epidermal growth factor (EGF) and insulin on gastric epithelial restoration in vitro. Rabbit gastric epithelial cells were cultured and formed a complete monolayer cell sheet in 2 days. We created a wound (1.8 +/- 0.05 mm2) by denuding an area of cells, and EGF (0.1-30 ng/ml) and/or insulin (1 nM-1 microM) was added. The restoration process, which included cell migration and proliferation, was monitored by measuring the cell-free area every 12 h for 2 days. Proliferating cells were detected by sequential staining with bromodeoxyuridine (BrdU). Control cells showed complete repair in 36-48 h and restoration was accelerated dose-dependently by EGF or insulin. EGF plus insulin further accelerated restoration, which was then completed in 12-24 h. EGF and/or insulin increased the number of BrdU- positive cells. The results indicated that EGF and insulin additively accelerated gastric epithelial wound repair by stimulating both the migration and the proliferation of gastric epithelial cells (particularly the former).
Maehiro
K
K
Department of Gastroenterology, Juntendo University School of Medicine, Tokyo, Japan.
Watanabe
S
S
Hirose
M
M
Iwazaki
R
R
Miwa
H
H
Sato
N
N
D000818:Animals; D002455:Cell Division / Q000187:drug effects*; D002465:Cell Movement / Q000187:drug effects*; D002478:Cells, Cultured / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D004359:Drug Therapy, Combination; D004815:Epidermal Growth Factor / Q000494:pharmacology*; D004847:Epithelial Cells / Q000187:drug effects / Q000473:pathology*; D005753:Gastric Mucosa / Q000187:drug effects / Q000293:injuries / Q000473:pathology*; D007004:Hypoglycemic Agents / Q000494:pharmacology*; D007328:Insulin / Q000494:pharmacology*; D008297:Male; D008858:Microscopy, Phase-Contrast; D011817:Rabbits; D014945:Wound Healing / Q000187:drug effects
D007004:Hypoglycemic Agents; D007328:Insulin; D004815:Epidermal Growth Factor