title: Deciphering protein sequence information through hydrophobic cluster analysis (HCA): current status and perspectives.
abstract: Ten years after the idea of hydrophobic cluster analysis (HCA) was conceived and first published, theoretical and practical experience has shown this unconventional method of protein sequence analysis to be particularly efficient and sensitive, especially with families of sequences sharing low levels of sequence identity. This extreme sensitivity has made it possible to predict the functions of genes whose sequence similarities are hardly if at all detectable by current one-dimensional (1D) methods alone, and offers a new way to explore the enormous amount of data generated by genome sequencing. HCA also provides original tools to understand fundamental features of protein stability and folding. Since the last review of HCA published in 1990 [1], significant improvements have been made and several new facets have been addressed. Here we wish to update and summarize this information.
authors.affiliation: Systèmes Moléculaires et Biologie Structurale, LMCP, CNRS URA 09, UP6/UP7, Paris, France. Isabelle.Callebaut@lmcp.jussieu.fr
authors.forename: I
authors.initials: I
authors.lastname: Callebaut
authors.forename: G
authors.initials: G
authors.lastname: Labesse
authors.forename: P
authors.initials: P
authors.lastname: Durand
authors.forename: A
authors.initials: A
authors.lastname: Poupon
authors.forename: L
authors.initials: L
authors.lastname: Canard
authors.forename: J
authors.initials: J
authors.lastname: Chomilier
authors.forename: B
authors.initials: B
authors.lastname: Henrissat
authors.forename: J P
authors.initials: JP
authors.lastname: Mornon
mesh_terms: D000595:Amino Acid Sequence; D055598:Chemical Phenomena; D002627:Chemistry, Physical; D006801:Humans; D008958:Models, Molecular; D008969:Molecular Sequence Data; D015394:Molecular Structure; D011506:Proteins / Q000737:chemistry*; D017421:Sequence Analysis / Q000379:methods*; D000515:alpha 1-Antitrypsin / Q000737:chemistry
chemical_list: D011506:Proteins; D000515:alpha 1-Antitrypsin