Adenosine A3 receptor stimulation inhibits migration of human eosinophils.
Activation of adenosine A3 receptors (A3-R) produced a dose-dependent reduction in the chemotaxis of human eosinophils to platelet-activating factor (PAF), RANTES, and leukotriene B4 (LTB4) to a maximum of 58, 48, and 52%, respectively (P < 0.02). This effect was completely reversed by selective A3-R antagonists. In contrast, activation of A1 or A2a-R did not affect PAF-induced eosinophil chemotaxis. PAF up-regulated the expression of CDllb/CD18, down-regulated L-selectin, and also increased F-actin assembly in eosinophils. The expression of these activation markers was not influenced by A3-R, A2a, or A1-R stimulation. Activation of A3-R may play an important role in inflammation by inhibiting eosinophil migration.
Knight
D
D
University of British Columbia Pulmonary Research Laboratory, St. Paul's Hospital, Vancouver, Canada.
Zheng
X
X
Rocchini
C
C
Jacobson
M
M
Bai
T
T
Walker
B
B
D000241:Adenosine / Q000031:analogs & derivatives* / Q000494:pharmacology*; D018821:CD18 Antigens / Q000096:biosynthesis; D018946:Chemokine CCL5 / Q000494:pharmacology; D002634:Chemotaxis, Leukocyte / Q000187:drug effects*; D004804:Eosinophils / Q000187:drug effects / Q000502:physiology*; D006801:Humans; D066298:In Vitro Techniques; D019041:L-Selectin / Q000096:biosynthesis; D007975:Leukotriene B4 / Q000494:pharmacology; D016177:Macrophage-1 Antigen / Q000096:biosynthesis; D010972:Platelet Activating Factor / Q000494:pharmacology; D043684:Receptor, Adenosine A3; D018047:Receptors, Purinergic P1 / Q000187:drug effects / Q000502:physiology*
D018821:CD18 Antigens; D018946:Chemokine CCL5; D016177:Macrophage-1 Antigen; D010972:Platelet Activating Factor; D043684:Receptor, Adenosine A3; D018047:Receptors, Purinergic P1; D019041:L-Selectin; D007975:Leukotriene B4; D000241:Adenosine; C090034:2-chloro-N(6)-(3-iodobenzyl)adenosine-5'-N-methyluronamide