Establishment of a murine model for metastasis of cytokine-producing tumor to the brain.
The A375 cell line, derived from human malignant melanoma, has characteristics of interleukin-6 (IL-6) production. By using this cell line, we have investigated a murine metastasis model of IL-6-producing tumors to the brain by injecting A375 cells directly into the left cardiac ventricle. Nude mice were anesthetized with intraperitoneal injection of pentobarbital sodium. Next, A375 cells suspended in phosphate-buffered saline (PBS) were injected into the left cardiac ventricle of mice. An intracardiac injection of 10(5) cells developed tumor colonies in the brain after 4 to 6 weeks. Metastatic cells were found in every lobe of the brain. An immunocytochemical study revealed IL-6 production by A375 cells at the metastatic sites in the brain. By the transfection of genes encoding proteins into A375 cells, a novel model of protein expression in the brain in vivo could be constructed. Our system does not require great skill. Our experimental model will facilitate future studies of the local effects of proteins in the brain.
Nakai
M
M
Hyogo Institute for Aging Brain and Cognitive Disorders, Himeji, Japan.
Takauchi
S
S
Yamaguchi
T
T
Kawamata
T
T
Maeda
K
K
Tanaka
C
C
D000818:Animals; D001923:Brain Chemistry; D001932:Brain Neoplasms / Q000378:metabolism / Q000556:secondary*; D004195:Disease Models, Animal; D015972:Gene Expression Regulation, Neoplastic / Q000502:physiology; D007273:Injections, Intramuscular; D015850:Interleukin-6 / Q000032:analysis / Q000235:genetics / Q000378:metabolism*; D008297:Male; D008545:Melanoma / Q000378:metabolism / Q000556:secondary*; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D009368:Neoplasm Transplantation; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000378:metabolism; D012878:Skin Neoplasms / Q000473:pathology
D015850:Interleukin-6; D012333:RNA, Messenger