title: Pregnancy diagnosis in dairy goats using progesterone assay kits and oestrous observation.
abstract: Two qualitative on-farm milk progesterone test kits were used for early pregnancy diagnosis in goats. One kit was based on latex agglutination (LA) and the other on enzyme-linked immunosorbent assay (ELISA). The accuracy of early pregnancy diagnosis by these two methods was compared with the accuracy of oestrous observation (OeO) and the level of plasma progesterone measured by radioimmunoassay (RIA). Dairy goats (n = 73) from a single herd were used for collection of milk and blood samples 20 days after breeding. Ultrasound examination 50 days after mating found that 49 (67%) goats were pregnant, and 24 (33%) were not pregnant. Using ultrasound as a reference method, the accuracy of early pregnancy diagnosis by RIA and OeO was 92% and 86%, respectively, and for non-pregnancy 100% for both RIA and OeO. The accuracy of early pregnancy diagnosis by ELISA and LA was 82% and 79% and for non-pregnancy, 88% and 100%, respectively. The kappa-statistic for RIA, OeO, ELISA and LA was 0.93, 0.84, 0.77 and 0.73, respectively. It was concluded that LA and ELISA tests can be used for early pregnancy diagnosis in dairy goats. However, in the herd studied, early pregnancy diagnosis by OeO was as good as that achieved with progesterone determination using the kits.
authors.affiliation: Department of Reproduction and Forensic Medicine, Norwegian College of Veterinary Medicine, Oslo, Norway.
authors.forename: I V
authors.initials: IV
authors.lastname: Engeland
authors.forename: E
authors.initials: E
authors.lastname: Ropstad
authors.forename: O
authors.initials: O
authors.lastname: Andresen
authors.forename: L O
authors.initials: LO
authors.lastname: Eik
mesh_terms: D000818:Animals; D002417:Cattle; D004797:Enzyme-Linked Immunosorbent Assay / Q000662:veterinary; D004972:Estrus Detection; D005260:Female; D006041:Goats / Q000502:physiology*; D008892:Milk / Q000737:chemistry*; D011247:Pregnancy; D011258:Pregnancy Tests / Q000379:methods / Q000662:veterinary*; D011374:Progesterone / Q000032:analysis* / Q000097:blood; D011863:Radioimmunoassay / Q000662:veterinary; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
chemical_list: D011374:Progesterone