Ethanol-induced cell death by lipid peroxidation in PC12 cells.
Free radical generation is hypothesized to be the cause of alcohol-induced tissue injury. Using fluorescent cis-parinaric acid and TBARS, lipid peroxidation was shown to be increased in the presence of trace amounts of free ferrous ion in PC12 cells. This increase in lipid peroxidation was enhanced by ethanol in a dose dependent manner and also correlated with loss of cell viability, as measured by increased release of lactate dehydrogenase (LDH). Resveratrol, a potent antioxidant, had a protective effect against lipid peroxidation and cell death. These findings strongly suggest that ethanol-induced tissue injury and cell death is a free radical mediated process, and may be important in alcohol-related premature aging and other degenerative diseases.
Sun
A Y
AY
Department of Pharmacology, University of Missouri, Columbia 65212, USA. aysun@muccmail.missouri.edu
Chen
Y M
YM
James-Kracke
M
M
Wixom
P
P
Cheng
Y
Y
D000818:Animals; D000975:Antioxidants / Q000494:pharmacology; D016923:Cell Death; D000431:Ethanol / Q000494:pharmacology*; D005231:Fatty Acids, Unsaturated; D005296:Ferrous Compounds / Q000494:pharmacology; D005456:Fluorescent Dyes; D007502:Iron Chelating Agents / Q000494:pharmacology; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D015227:Lipid Peroxidation / Q000187:drug effects* / Q000502:physiology; D009474:Neurons / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D016716:PC12 Cells; D004369:Pentetic Acid / Q000494:pharmacology; D051381:Rats; D000077185:Resveratrol; D013050:Spectrometry, Fluorescence; D013267:Stilbenes / Q000494:pharmacology; D017392:Thiobarbituric Acid Reactive Substances / Q000378:metabolism
D000975:Antioxidants; D005231:Fatty Acids, Unsaturated; D005296:Ferrous Compounds; D005456:Fluorescent Dyes; D007502:Iron Chelating Agents; D013267:Stilbenes; D017392:Thiobarbituric Acid Reactive Substances; D000431:Ethanol; D004369:Pentetic Acid; D007770:L-Lactate Dehydrogenase; C013641:parinaric acid; D000077185:Resveratrol; C029451:ferrous chloride