A novel stereotaxic-like method for injecting into the lateral ventricles of small passerine birds.
Recently, there has been an increase in the interest in various aspects of the biology of small passerine birds. In order to apply many modern neurobiological techniques, it would be useful to be able to surgically inject hormones and drugs directly into passerine brains. We describe a novel and cost-effective stereotaxic-like technique for injecting substances into the lateral ventricles of small passerine birds. The technique is rapid, reducing the period of anesthesia, and reliable. In addition, using this technique, we injected [3H]cysteine (to label newly synthesized proteins) with small doses of colchicine. Colchicine decreased the content of newly synthesized protein in the median eminence, thus suggesting that colchicine inhibited protein transport. The technique should prove useful in a number of neurobiologial applications.
Romero
L M
LM
Department of Zoology, University of Washington, Seattle 98195, USA. mromero@Tufts.edu
Wingfield
J C
JC
D000818:Animals; D001717:Birds / Q000502:physiology*; D001921:Brain / Q000187:drug effects* / Q000502:physiology; D001931:Brain Mapping / Q000295:instrumentation; D003078:Colchicine / Q000494:pharmacology; D003362:Cost-Benefit Analysis; D007276:Injections, Intraventricular / Q000191:economics / Q000295:instrumentation*; D008473:Median Eminence / Q000187:drug effects / Q000502:physiology; D009419:Nerve Tissue Proteins / Q000378:metabolism*; D013238:Stereotaxic Techniques / Q000191:economics / Q000295:instrumentation*
D009419:Nerve Tissue Proteins; D003078:Colchicine