Liver cysteine proteinases in macrophage depression induced by gadolinium chloride.
Liver lysosomal enzymes during macrophage depression (gadolinium chloride, 7 mg/kg, intravenously) and macrophage stimulation (zymosan, 100 mg/kg, intravenously) have been studied. It was shown that gadolinium chloride treatment of rats reduced the rate of carbon particles phagocytosis at 24 and 48 h after the single administration. Decreased endocytic capacity of Kupffer cells was confirmed also by electron microscopy. Gadolinium chloride induced labilization of liver lysosomes (increased free activity of cathepsins B and L); there was no changes of specific activity of liver cysteine proteinases (24 h). Gadolinium chloride prevented death of rats after administration of non-sonicated particular zymosan particles, resulting to 70% survival, compare with the 17% survival in group with zymosan alone. We can summarize that macrophages depression by gadolinium chloride abolish symptoms of inflammation in zymosan-model, influencing on cysteine proteinases of Kupffer cells.
Korolenko
T
T
Laboratory of Cellular Biochemistry, Institute of Physiology RAMS, Novosibirsk, Russia.
Svechnikova
I
I
Urazgaliyev
K
K
Vakulin
G
G
Djanaeva
S
S
D000818:Animals; D000893:Anti-Inflammatory Agents / Q000008:administration & dosage*; D003546:Cysteine Endopeptidases / Q000032:analysis*; D005682:Gadolinium / Q000008:administration & dosage*; D007275:Injections, Intravenous; D008099:Liver / Q000201:enzymology*; D008262:Macrophage Activation / Q000187:drug effects*; D008264:Macrophages / Q000187:drug effects / Q000201:enzymology*; D008297:Male; D051381:Rats; D017208:Rats, Wistar
D000893:Anti-Inflammatory Agents; D005682:Gadolinium; D003546:Cysteine Endopeptidases; C038958:gadolinium chloride