title: Cloning and sequencing of a second laccase gene from the white-rot fungus Coriolus versicolor.
abstract: A second laccase gene, CVLG1, was isolated from Coriolus versicolor. CVLGI encodes a precursor protein of 526 amino acids which contains a 23-amino acid signal sequence, and the coding region is interrupted by 11 introns. The number of potential N-glycosylation sites in this product is 12 and the greatest among that of polyporales laccases. Moreover, this protein shares about 70% homology with other polyporales laccases. Genomic Southern analysis showed that C. versicolor laccases are encoded by more than four genes including CVLG1 and a transposed allele of this gene.
authors.affiliation: Laboratory of Wood Chemistry, Faculty of Agriculture, Tokyo University of Agriculture and Technology, Japan.
authors.forename: J
authors.initials: J
authors.lastname: Mikuni
authors.forename: N
authors.initials: N
authors.lastname: Morohoshi
mesh_terms: D000483:Alleles; D000595:Amino Acid Sequence; D001483:Base Sequence; D001487:Basidiomycota / Q000201:enzymology / Q000235:genetics; D003001:Cloning, Molecular; D004271:DNA, Fungal / Q000235:genetics; D004792:Enzyme Precursors / Q000737:chemistry / Q000235:genetics; D005800:Genes, Fungal; D006031:Glycosylation; D007438:Introns; D042845:Laccase; D008969:Molecular Sequence Data; D010088:Oxidoreductases / Q000737:chemistry / Q000235:genetics*; D011124:Polyporaceae / Q000201:enzymology* / Q000235:genetics*; D021382:Protein Sorting Signals / Q000737:chemistry / Q000235:genetics; D017386:Sequence Homology, Amino Acid; D013045:Species Specificity
chemical_list: D004271:DNA, Fungal; D004792:Enzyme Precursors; D021382:Protein Sorting Signals; D010088:Oxidoreductases; D042845:Laccase