Site-directed mutagenesis: a two-step method using PCR and DpnI.
Li
S
S
Department of Immunology, University of Texas M.D. Anderson Cancer Center, Houston 77030, USA.
Wilkinson
M F
MF
D000818:Animals; D015252:Deoxyribonucleases, Type II Site-Specific / Q000378:metabolism*; D051379:Mice; D016297:Mutagenesis, Site-Directed; D016133:Polymerase Chain Reaction / Q000379:methods*; D016693:Receptors, Antigen, T-Cell, alpha-beta / Q000235:genetics
D016693:Receptors, Antigen, T-Cell, alpha-beta; C020102:endodeoxyribonuclease DpnI; D015252:Deoxyribonucleases, Type II Site-Specific