Detection of circulating donor deoxyribonucleic acid by microsatellite analysis in a liver transplant recipient.
The diagnosis of graft-versus-host disease following liver transplantation may be delayed because the clinical and pathological features are nonspecific. We report the use of microsatellites to support a diagnosis of GVHD in a patient who developed fever and a skin rash 28 days after liver transplantation. The pattern of microsatellite alleles amplified from the peripheral blood on day 51 posttransplant indicated that recipient and donor DNA were present in approximately equal proportions. Microsatellite typing is a simple and rapid method to identify high levels of circulating donor DNA to support a diagnosis of GVHD following liver transplantation.
Burt
M
M
Liver Unit, Queensland Institute of Medical Research, Brisbane, Australia.
Jazwinska
E
E
Lynch
S
S
Kerlin
P
P
Gill
D
D
Steadman
C
C
Jonsson
J
J
Strong
R
R
Powell
E
E
D000483:Alleles; D018076:DNA, Complementary / Q000032:analysis*; D017809:Fatal Outcome; D005260:Female; D006086:Graft vs Host Disease / Q000175:diagnosis* / Q000276:immunology; D006680:HLA Antigens / Q000032:analysis* / Q000276:immunology; D006801:Humans; D016031:Liver Transplantation / Q000009:adverse effects*; D018895:Microsatellite Repeats; D008875:Middle Aged; D016133:Polymerase Chain Reaction; D012680:Sensitivity and Specificity
D018076:DNA, Complementary; D006680:HLA Antigens