Capillary electrophoresis.
Capillary electrophoresis is a rapid and versatile electrophoretic technique that has found several applications in the field of peptide synthesis. The high resolution and charge-based separation capabilities make it very complementary to RP-HPLC. In addition, both are essentially quantitative, which makes them ideal as purity assessment techniques. Capillary electrophoresis can be used to monitor any charge-related changes in synthetic peptides such as incomplete deprotection or chemical modifications. Capillary electrophoresis can also be very sensitive to changes in the shape or size of a peptide. However, its greatest value for the characterization of synthetic peptides is when it is used in combination with other analytical techniques such as mass spectrometry, HPLC, and amino acid analysis.
Sanchez
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A
Beckman Center, Stanford University, California 94305-5425, USA.
Smith
A J
AJ
D000595:Amino Acid Sequence; D019075:Electrophoresis, Capillary / Q000379:methods*; D008969:Molecular Sequence Data; D010455:Peptides / Q000032:analysis / Q000138:chemical synthesis / Q000737:chemistry*
D010455:Peptides