Rapid PCR amplification of chimeric products and its direct application to in vivo [corrected] testing of recombinant DNA construction strategies.
This article describes a simple and rapid method for efficient production of chimeric products by polymerase chain reaction (PCR). This protocol is amenable to site-directed mutagenesis strategies and can be done without the time-consuming gel purification step. The PCR products generated can also be directly used for direct gene transfer into plant cells without further subcloning to test construction strategies.
Cho
M J
MJ
Department of Plant and Microbial Biology, University of California, Berkeley 94720, USA. mjcho@nature.berkely.edu
Lemaux
P G
PG
D001483:Base Sequence; D017931:DNA Primers; D004274:DNA, Recombinant / Q000235:genetics*; D016297:Mutagenesis, Site-Directed; D016133:Polymerase Chain Reaction / Q000379:methods*; D011993:Recombinant Fusion Proteins / Q000235:genetics*
D017931:DNA Primers; D004274:DNA, Recombinant; D011993:Recombinant Fusion Proteins