title: Stress proteins are not induced in mammalian cells exposed to radiofrequency or microwave radiation.
abstract: The induction of stress proteins in HeLa and CHO cells was investigated following a 2 h exposure to radiofrequency (RF) or microwave radiation. Cells were exposed or sham exposed in vitro under isothermal (37 +/- 0.2 degrees C) conditions. HeLa cells were exposed to 27- or 2450 MHz continuous wave (CW) radiation at a specific absorption rate (SAR) of 25 W/kg. CHO cells were exposed to CW 27 MHz radiation at a SAR of 100 W/kg. Parallel positive control studies included 2 h exposure of HeLa or CHO cells to 40 degrees C or to 45 microM cadmium sulfate. Stress protein induction was assayed 24 h after treatment by electrophoresis of whole-cell extracted protein labeled with [35S]-methionine. Both cell types exhibited well-characterized responses to the positive control stresses. Under these exposure conditions, neither microwave nor RF radiation had a detectable effect on stress protein induction as determined by either comparison of RF-exposed cells with sham-exposed cells or comparison with heat-stressed or Cd++ positive control cells.
authors.affiliation: Department of Physiology and Biophysics, Medical College of Virginia, Virginia Commonwealth University, Richmond 23298, USA.
authors.forename: S F
authors.initials: SF
authors.lastname: Cleary
authors.forename: G
authors.initials: G
authors.lastname: Cao
authors.forename: L M
authors.initials: LM
authors.lastname: Liu
authors.forename: P M
authors.initials: PM
authors.lastname: Egle
authors.forename: K R
authors.initials: KR
authors.lastname: Shelton
mesh_terms: D000818:Animals; D016466:CHO Cells; D002104:Cadmium / Q000633:toxicity; D006224:Cricetinae; D006367:HeLa Cells; D006360:Heat-Shock Proteins / Q000096:biosynthesis*; D006358:Hot Temperature / Q000009:adverse effects; D006801:Humans; D008872:Microwaves / Q000009:adverse effects*; D011846:Radio Waves / Q000009:adverse effects*
chemical_list: D006360:Heat-Shock Proteins; D002104:Cadmium