title: NOS type-1 mRNA expression and protein localization in spinal autonomic neurons.
abstract: Autonomic neurons of the rat spinal cord show strong NADPH diaphorase activity and immunoreactivity for nitric oxide synthase (NOS). Here we show mRNA expression of NOS type-1 (neuronal or brain NOS) transcripts in cell bodies of sympathetic preganglionic neurons (SPNs) of the intermediolateral (IML) cell column by non-radioactive in situ hybridization using NOS-I riboprobes. Hybridization signals occurred only in neuronal cell bodies and not outside, in what appeared to be fibers and/or terminals. In preganglionic fibers of SPNs, however, dense axoplasmic immunogold labeling was detected with a monoclonal anti-NOS-I antibody. Expression of NOS-I mRNA in SPN cell bodies and axoplasmic immunolocalization of NOS-I protein suggest that protein translocation is involved in NO-mediated preganglionic control of peripheral targets.
authors.affiliation: Department of Anatomy and Cell Biology, Humboldt University Hospital (Charité), Berlin-Charlottenburg, Germany.
authors.forename: M
authors.initials: M
authors.lastname: Vogel
authors.forename: G
authors.initials: G
authors.lastname: Lück
authors.forename: S
authors.initials: S
authors.lastname: Bachmann
authors.forename: D
authors.initials: D
authors.lastname: Blottner
mesh_terms: D000818:Animals; D001341:Autonomic Nervous System / Q000033:anatomy & histology / Q000201:enzymology / Q000378:metabolism*; D001369:Axons / Q000201:enzymology / Q000378:metabolism; D015153:Blotting, Western; D004076:Digoxigenin / Q000378:metabolism; D007150:Immunohistochemistry; D017403:In Situ Hybridization; D007527:Isoenzymes / Q000096:biosynthesis*; D016253:Microscopy, Immunoelectron; D009419:Nerve Tissue Proteins / Q000378:metabolism*; D009474:Neurons / Q000201:enzymology / Q000378:metabolism*; D019001:Nitric Oxide Synthase / Q000096:biosynthesis*; D015347:RNA Probes; D012333:RNA, Messenger / Q000096:biosynthesis*; D051381:Rats; D017208:Rats, Wistar; D013116:Spinal Cord / Q000033:anatomy & histology / Q000201:enzymology / Q000378:metabolism*
chemical_list: D007527:Isoenzymes; D009419:Nerve Tissue Proteins; D015347:RNA Probes; D012333:RNA, Messenger; D019001:Nitric Oxide Synthase; D004076:Digoxigenin