Bovine herpesvirus 4 BORFE2 protein inhibits Fas- and tumor necrosis factor receptor 1-induced apoptosis and contains death effector domains shared with other gamma-2 herpesviruses.
Fas- and tumor necrosis factor receptor 1 (TNFR1)-induced apoptosis is mediated by the interaction of FADD with caspase-8. Here, we report that the bovine herpesvirus 4 (BHV4) BORFE2 gene encodes a protein that inhibits Fas- and TNFR1-induced apoptosis and contains death effector domains (DEDs). Using the yeast two-hybrid system, we found that the BORFE2 protein interacts with the prodomain of caspase-8. Furthermore, we show that BHV4 BORFE2 is a member of a family of DED-containing proteins that includes other gamma-2 herpesviruses, such as Kaposi's sarcoma-associated herpesvirus and herpesvirus saimiri.
Wang
G H
GH
Laboratory of Clinical Investigation, National Institute of Allergy and Infectious Diseases, Bethesda, Maryland 20892, USA. gwang@atlas.niaid.nih.gov
Bertin
J
J
Wang
Y
Y
Martin
D A
DA
Wang
J
J
Tomaselli
K J
KJ
Armstrong
R C
RC
Cohen
J I
JI
D000595:Amino Acid Sequence; D000818:Animals; D017209:Apoptosis; D020170:Caspase 1; D002417:Cattle / Q000821:virology; D003546:Cysteine Endopeptidases / Q000378:metabolism; D018142:Gammaherpesvirinae / Q000737:chemistry / Q000235:genetics / Q000472:pathogenicity*; D011485:Protein Binding; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid; D014764:Viral Proteins / Q000737:chemistry* / Q000235:genetics
C108724:BORFE2 protein, Bovine herpesvirus 4; D014764:Viral Proteins; D003546:Cysteine Endopeptidases; D020170:Caspase 1