Adherence of oral streptococci to an immobilized antimicrobial agent.
An antimicrobial agent, 3-(trimethoxysilyl)-propyldimethyloctadecyl ammonium chloride, was immobilized on silica. Interaction between the material (termed) OAIS) and various oral bacterial species were then studied. Seven species of Streptococcus and two Actinomyces were investigated for their ability to adhere to this biomaterial. Cell-surface hydrophobicity and zeta-potential were examined as well. Analysis of extracted hydrophobic proteins which adhered to OAIS revealed that the adherence of these micro-organisms was closely related to the hydrophobicity of their cell surfaces. The results of zeta-potential assays indicated that negative charge on the cell surface inhibited adherence to OAIS. Gel electrophoresis revealed that OAIS could absorb cell-surface hydrophobic proteins from all bacterial species tested. Preadsorption of hydrophobic components on the cell surface inhibited adherence of the Strep. mutans strain to OAIS in a dose-dependent manner. The results indicate that OAIS adsorption of these oral bacteria was dependent on the degree of hydrophobicity of their surfaces. A major component of this adherence was hydrophobic cell-surface proteins.
Saito
T
T
Research and Development Department, Sunstar Inc., Osaka, Japan.
Takatsuka
T
T
Kato
T
T
Ishihara
K
K
Okuda
K
K
D000042:Absorption; D000190:Actinomyces / Q000187:drug effects; D016924:Actinomyces viscosus / Q000187:drug effects; D000327:Adsorption; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D001422:Bacterial Adhesion / Q000187:drug effects*; D001426:Bacterial Proteins / Q000032:analysis / Q000502:physiology; D001672:Biocompatible Materials / Q000494:pharmacology; D002473:Cell Wall / Q000502:physiology; D004305:Dose-Response Relationship, Drug; D004591:Electrophoresis, Polyacrylamide Gel; D004594:Electrophysiology; D006801:Humans; D009055:Mouth / Q000382:microbiology; D017646:Organosilicon Compounds / Q000494:pharmacology*; D000644:Quaternary Ammonium Compounds / Q000494:pharmacology*; D012822:Silicon Dioxide; D012967:Sodium Dodecyl Sulfate; D013291:Streptococcus / Q000187:drug effects*; D013295:Streptococcus mutans / Q000187:drug effects; D019533:Streptococcus oralis / Q000187:drug effects; D013298:Streptococcus sanguis / Q000187:drug effects; D016986:Streptococcus sobrinus / Q000187:drug effects; D013501:Surface-Active Agents; D014867:Water
D000900:Anti-Bacterial Agents; D001426:Bacterial Proteins; D001672:Biocompatible Materials; D017646:Organosilicon Compounds; D000644:Quaternary Ammonium Compounds; D013501:Surface-Active Agents; D014867:Water; C018408:3-(trimethoxysilyl)propyldimethyloctadecylammonium; D012967:Sodium Dodecyl Sulfate; D012822:Silicon Dioxide