title: [Immunohistochemical detection of myocardial hypoxia].
abstract: Antibodies against myoglobin and fibrinogen were used for immunohistochemical investigation of myocardium in a group of 45 persons deceased of natural and violent cause. Suffocated persons showed a decrease or disappearing of myoglobin in 66% and disseminated positivity for fibrinogen in myocardial fibres in 70%. Deceased of violent cause without hypoxia had disseminated decrease or disappearing of myoglobin and simultaneous disseminated positive reaction for fibrinogen but in 14% of cases. Deceased persons with macroscopical heart infarction were used for control and had focal lack of myoglobin in 100%. Immunohistochemical investigation of myoglobin and fibrinogen can be used as a very sensitive prove of prolongated hypoxia or very intensive asphyxia when positive signs can occur after a very short period.
authors.affiliation: Ustav soudního lékarství 3. LF UK, Praha.
authors.forename: P
authors.initials: P
authors.lastname: Toupalík
mesh_terms: D000293:Adolescent; D000328:Adult; D001237:Asphyxia / Q000175:diagnosis / Q000378:metabolism; D005340:Fibrinogen / Q000032:analysis* / Q000276:immunology; D005554:Forensic Medicine / Q000379:methods; D006801:Humans; D000860:Hypoxia / Q000175:diagnosis* / Q000378:metabolism; D007150:Immunohistochemistry; D008875:Middle Aged; D009206:Myocardium / Q000378:metabolism*; D009211:Myoglobin / Q000032:analysis* / Q000276:immunology
chemical_list: D009211:Myoglobin; D005340:Fibrinogen