title: Rapid method for detection of point mutations using mismatch binding protein (MutS) and an optical biosensor.
abstract: This paper describes a new method for detecting DNA point mutations using a mismatch binding protein. The interactions of mismatches and mismatch binding proteins are detected by the optical biosensor technology based on surface plasmon resonance (SPR).
authors.affiliation: Research and Development Department, Pharmacia Biotech KK, Tokyo, Japan.
authors.forename: M
authors.initials: M
authors.lastname: Gotoh
authors.forename: M
authors.initials: M
authors.lastname: Hasebe
authors.forename: T
authors.initials: T
authors.lastname: Ohira
authors.forename: Y
authors.initials: Y
authors.lastname: Hasegawa
authors.forename: Y
authors.initials: Y
authors.lastname: Shinohara
authors.forename: H
authors.initials: H
authors.lastname: Sota
authors.forename: J
authors.initials: J
authors.lastname: Nakao
authors.forename: M
authors.initials: M
authors.lastname: Tosu
mesh_terms: D000251:Adenosine Triphosphatases; D000255:Adenosine Triphosphate / Q000737:chemistry; D001426:Bacterial Proteins; D015374:Biosensing Techniques; D004252:DNA Mutational Analysis / Q000379:methods*; D004273:DNA, Neoplasm / Q000032:analysis*; D004268:DNA-Binding Proteins; D029968:Escherichia coli Proteins; D006801:Humans; D051719:MutS DNA Mismatch-Binding Protein; D017354:Point Mutation; D014407:Tumor Cells, Cultured
chemical_list: D001426:Bacterial Proteins; D004273:DNA, Neoplasm; D004268:DNA-Binding Proteins; D029968:Escherichia coli Proteins; D000255:Adenosine Triphosphate; D000251:Adenosine Triphosphatases; D051719:MutS DNA Mismatch-Binding Protein; C497175:MutS protein, E coli