Modified polyacrylamide microspheres as immunosorbent.
Extracorporeal immunoadsorption system has been used for the specific removal of immunologically active substances from the blood. In this study, an attempt is made to utilise polyacrylamide microspheres as a matrix for immunoadsorption. Phenyl alanine is coupled onto the polymer beads using glutaraldehyde. These modified beads exhibit a high binding affinity for gamma-globulin compared to bare beads. The surface modified ones showed selective adsorption of immunoglobulins of IgG class. The C3 adsorption pattern is not altered significantly upon modification. Modified beads are found to be less hemolytic after modification. However, more studies are to be conducted for the development of a hemoperfusion column based on this modified matrix.
Panikkar
B
B
Division of Biosurface Technology, Sree Chitra Tirunal Institute for Medical Sciences & Technology, Trivandrum, India.
Hari
P R
PR
Sharma
C P
CP
D000180:Acrylic Resins / Q000737:chemistry*; D000818:Animals; D002417:Cattle; D002846:Chromatography, Affinity; D003432:Cross-Linking Reagents / Q000737:chemistry*; D004591:Electrophoresis, Polyacrylamide Gel; D006801:Humans; D007163:Immunosorbent Techniques; D007164:Immunosorbents / Q000737:chemistry*; D008863:Microspheres; D010477:Perfusion; D000099166:Polyacrylamides
D000180:Acrylic Resins; D003432:Cross-Linking Reagents; D007164:Immunosorbents; D000099166:Polyacrylamides