Effects of S-adenosyl-L-methionine on blood platelet activation.
1. In rats treated with S-adenosyl-L-methionine (SAMe), platelet aggregation in whole blood was inhibited by 50-57% with respect to control values. 2. In whole blood, SAMe also inhibited platelet aggregation, particularly when aggregation was induced with collagen (maximum inhibition 78.6 +/- 5.8% with 10(-5) M SAMe). 3. The antiaggregant response was seen with SAMe in the range of concentrations from 10(-7) to 10(-5) M, whereas concentrations in the range from 10(-4) to 10(-3) M had a progressively weaker effect. 4. Both red blood cells and leukocytes enhanced the antiaggregant effect of SAMe, as did simultaneous incubation with L-arginine. 5. The intraplatelet concentration of glutathione also was increased by SAMe.
De la Cruz
J P
JP
Department of Pharmacology and Therapeutics, School of Medicine, University of Malaga, Spain.
Mérida
M
M
González-Correa
J A
JA
Ortiz
P
P
Sánchez de la Cuesta
F
F
D000241:Adenosine / Q000097:blood; D000328:Adult; D000818:Animals; D003094:Collagen / Q000494:pharmacology; D017097:Electric Impedance; D004912:Erythrocytes / Q000378:metabolism; D005978:Glutathione / Q000097:blood; D006801:Humans; D066298:In Vitro Techniques; D008297:Male; D015539:Platelet Activation / Q000187:drug effects*; D010974:Platelet Aggregation / Q000187:drug effects; D010975:Platelet Aggregation Inhibitors / Q000494:pharmacology*; D051381:Rats; D012436:S-Adenosylmethionine / Q000494:pharmacology*
D010975:Platelet Aggregation Inhibitors; D012436:S-Adenosylmethionine; D003094:Collagen; D005978:Glutathione; D000241:Adenosine