Active-specific immunotherapy of pancreatic carcinoma: usefulness of human pancreatic carcinomas in preparing autologous tumor vaccines.
Using a mouse model we investigate whether pancreatic carcinoma cells can serve as a basic material for the preparation of tumour vaccines. Human pancreatic carcinomas grown in nude mice were dissociated and stimulated with interferon-gamma and tocopherol acetate. Due to the very homogenous tumour material the yield of vital tumour cells even from small specimens was high enough to produce at least three vaccine doses each. Flow cytometric analyses of stimulated cells showed a significant increase in MHC I presenting cells compared to nonstimulated cells. These preliminary data suggest that beneath the successful application of tumor vaccines to renal carcinoma, melanoma, colon carcinoma and different gynaecological carcinomas pancreatic carcinoma could be a further candidate for this kind of therapy.
Grossmann
A
A
Macrolab GmbH, Hannover, Germany.
Rameken
M
M
Klapdor
R
R
D000818:Animals; D019496:Cancer Vaccines / Q000627:therapeutic use*; D015395:Histocompatibility Antigens Class I / Q000032:analysis; D006801:Humans; D016233:Immunotherapy, Active; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D010190:Pancreatic Neoplasms / Q000628:therapy*
D019496:Cancer Vaccines; D015395:Histocompatibility Antigens Class I