Separation of DL-dopa by means of micellar electrokinetic capillary chromatography after derivatization with Marfey's reagent.
Micellar electrokinetic capillary chromatography was used to separate levodopa (L-3,4-dihydroxyphenyl-L-alanine), dextrodopa (D-3,4-dihydroxyphenyl-L-alanine) and DL-3-O-methyl-dopa, a metabolite of dopa, which were derivatised with the Marfey's reagent, in sodium borate, sodium dodecyl sulfate and acetonitile buffer system. Whereas DL-dopa derivatives could be separated, DL-3-O-methyl-dopa derivatives could not.
Wu
G
G
Chair of Special Pharmacology, Medical School, University of Udine, Italy.
Furlanut
M
M
D002845:Chromatography; D004295:Dihydroxyphenylalanine / Q000302:isolation & purification*; D004586:Electrophoresis; D008823:Micelles
D008823:Micelles; D004295:Dihydroxyphenylalanine