Birth of a healthy female after intracytoplasmic sperm injection of cryopreserved human oocytes.
OBJECTIVE
To describe the first birth achieved after intracytoplasmic sperm injection (ICSI) of cryopreserved human oocytes.


METHODS
Case report.


METHODS
University of Bologna Hospital, Department of Obstetrics and Gynecology, Reproductive Endocrinology Unit, IVF and Infertility Center.


METHODS
One patient undergoing IVF.


METHODS
Transvaginal ultrasound-guided oocyte retrieval followed by oocyte freezing. Artificial preparation of the endometrium with E2 and P, oocyte thawing, and ICSI.


RESULTS
Four of 12 cryopreserved oocytes survived; using ICSI, 2 underwent normal fertilization but only 1 cleaved. One good-quality 4-cell embryo was transferred. A single gestation was confirmed by ultrasound at the 7th week. Amniocentesis was performed at the 16th week and demonstrated a normal female karyotype of 46,XX. After a normal pregnancy, a healthy female infant was born at the 38th week of gestation.


CONCLUSIONS
The combination of ICSI and oocyte cryopreservation is a new tool in assisted reproductive technology.
Porcu
E
E
Department of Obstetrics and Gynecology, University of Bologna, Italy.
Fabbri
R
R
Seracchioli
R
R
Ciotti
P M
PM
Magrini
O
O
Flamigni
C
C
D000328:Adult; D015925:Cryopreservation; D003593:Cytoplasm; D036861:Delivery, Obstetric; D004624:Embryo Transfer; D005260:Female; D005306:Fertilization; D006801:Humans; D008297:Male; D008845:Microinjections; D008846:Micromanipulation; D009865:Oocytes / Q000502:physiology*; D011247:Pregnancy; D012016:Reference Values; D013094:Spermatozoa