Interleukin-4 production in Epstein-Barr virus-transformed B cell lines from peripheral mononuclear cells of patients with atopic dermatitis.
Interleukin-4 (IL-4) is known as an immunomodulatory cytokine secreted by relatively few cell types, for example, activated T lymphocytes, basophils, and mast cells, but not by B cells. It plays an important role in promoting the production of the IgE antibody. We established Epstein-Barr virus (EBV)-positive B cell lines from peripheral blood mononuclear cells of patients with atopic dermatitis (AD) and tested the production of several cytokines in the cell lines. We found that IL-4 was produced in a cell line, OB, by an IL-4-specific enzyme-linked immunosorbent assay. IL-4 mRNA was detected in OB and two other AD-derived cell lines by IL-4-specific reverse transcription-polymerase chain reaction. IgE was also produced by the OB cells. The production of IL-4 and IgE was enhanced in the cells treated with 12-O-tetradecanoyl phorbol-13-acetate. This is the first evidence that IL-4 is produced by an EBV-transformed B cell line.
Ohnishi
E
E
Infectious Disease Surveillance Center, National Institute of Infectious Diseases, Tokyo, Japan.
Iwata
T
T
Inouye
S
S
Kurata
T
T
Sairenji
T
T
D001402:B-Lymphocytes / Q000378:metabolism* / Q000821:virology; D002461:Cell Line, Transformed; D002999:Clone Cells; D003876:Dermatitis, Atopic / Q000097:blood; D004854:Herpesvirus 4, Human / Q000302:isolation & purification*; D006801:Humans; D007073:Immunoglobulin E / Q000096:biosynthesis; D015847:Interleukin-4 / Q000096:biosynthesis* / Q000235:genetics; D007963:Leukocytes, Mononuclear / Q000378:metabolism*; D012333:RNA, Messenger / Q000032:analysis; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology
D012333:RNA, Messenger; D015847:Interleukin-4; D007073:Immunoglobulin E; D013755:Tetradecanoylphorbol Acetate