Quantification of cat's articular afferents containing calcitonin gene-related peptide or substance P innervating normal and acutely inflamed knee joints.
In cats with an acute (32 h) unilateral knee joint inflammation the proportion of calcitonin gene-related peptide-(CGRP) and substance P-(SP) immunoreactive articular afferents, retrogradely labelled by Fast Blue (FB), were determined using immunohistochemistry. The proportion of neurons containing CGRP was significantly higher on the inflamed side (52%) than on the contralateral side (39%) and in controls (42%). However, the proportion of SP-immunoreactive articular perikarya on the inflamed side (26%) did not differ from the contralateral side (24%) and the control cats (22%). These data indicate that acute inflammation induces the synthesis of CGRP but not of SP in joint afferents.
Hanesch
U
U
Physiologisches Institut der Universität Würzburg, Germany. ulrike.hanesch@rzroe.uni-wuerzburg.de
Heppelmann
B
B
Schmidt
R F
RF
D000208:Acute Disease; D000344:Afferent Pathways / Q000737:chemistry / Q000187:drug effects; D000818:Animals; D001168:Arthritis / Q000139:chemically induced* / Q000473:pathology; D015740:Calcitonin Gene-Related Peptide / Q000032:analysis*; D016022:Case-Control Studies; D002415:Cats; D005260:Female; D007719:Knee Joint / Q000294:innervation*; D008297:Male; D012016:Reference Values; D013373:Substance P / Q000032:analysis*
D013373:Substance P; D015740:Calcitonin Gene-Related Peptide