Application of immunohistochemistry and in situ hybridization for detection of bovine coronavirus in paraffin-embedded, formalin-fixed intestines.
A monoclonal antibody (MAb) (Z3A5) against spike protein subunit of bovine coronavirus (BCV) reacted with the virus in formalin-fixed intestines in an immunoperoxidase test. We found an 88% correlation between immunohistochemistry with Z3A5 and in situ hybridization with a BCV nucleoprotein cDNA probe. MAb Z3A5 reacted with 90 BCV isolates from the United States and was an effective reagent for the diagnosis of BCV.
Zhang
Z
Z
Department of Diagnostic Medicine-Pathobiology, College of Veterinary Medicine, Kansas State University, Manhattan 66506, USA.
Andrews
G A
GA
Chard-Bergstrom
C
C
Minocha
H C
HC
Kapil
S
S
D000818:Animals; D000911:Antibodies, Monoclonal; D002417:Cattle; D002418:Cattle Diseases; D003106:Colon / Q000473:pathology / Q000821:virology*; D018352:Coronavirus Infections / Q000473:pathology / Q000662:veterinary*; D017938:Coronavirus, Bovine / Q000145:classification / Q000302:isolation & purification; D015342:DNA Probes; D007150:Immunohistochemistry / Q000379:methods; D017403:In Situ Hybridization / Q000379:methods; D007413:Intestinal Mucosa / Q000473:pathology / Q000821:virology*; D008562:Membrane Glycoproteins / Q000032:analysis; D009336:Necrosis; D010232:Paraffin; D015203:Reproducibility of Results; D064370:Spike Glycoprotein, Coronavirus; D014481:United States; D014759:Viral Envelope Proteins / Q000032:analysis
D000911:Antibodies, Monoclonal; D015342:DNA Probes; D008562:Membrane Glycoproteins; D064370:Spike Glycoprotein, Coronavirus; D014759:Viral Envelope Proteins; D010232:Paraffin