Penicillin-binding protein sensitive to cephalexin in sporulation of Bacillus cereus.
Cephalexin, cefaclor, cefadroxil, and cefotaxime strongly inhibited sporulation of Bacillus cereus ts-4 at 1 microgram/ml. Cephalexin was most inhibitory on the sporulation of B. cereus when the antibiotic was added at 3 h after induction of sporulation by nutrient downshift technique. Examination of 4',6-diamidino-2-phenylindole-stained cells by fluorescence-phase contrast microscopy showed that cephalexin inhibited the formation of asymmetric septum. By using [3H]penicillin, eight penicillin-binding proteins (PBPs) were detected from the cells of B. cereus ts-4. Among them, four PBPs were also detected in sporulating cells. Affinity of cephalexin to PBPs were measured indirectly by competition for subsequent binding of radioactive penicillin G. Cephalexin strongly bound to PBP 4 with molecular weight of 72,000 in sporulating cells.
Miyamoto
T
T
Faculty of Agriculture, Kyushu University, Fukuoka, Japan.
Yamaguchi
K
K
Abu Sayed
M
M
Sasahara
R
R
Honjoh
K
K
Hatano
S
S
D001409:Bacillus cereus / Q000737:chemistry / Q000187:drug effects* / Q000502:physiology; D001426:Bacterial Proteins; D002352:Carrier Proteins / Q000032:analysis / Q000187:drug effects*; D002506:Cephalexin / Q000378:metabolism / Q000494:pharmacology*; D006602:Hexosyltransferases; D008970:Molecular Weight; D002267:Muramoylpentapeptide Carboxypeptidase / Q000032:analysis / Q000187:drug effects*; D046915:Penicillin-Binding Proteins; D010458:Peptidyl Transferases; D013171:Spores, Bacterial / Q000187:drug effects / Q000502:physiology
D001426:Bacterial Proteins; D002352:Carrier Proteins; D046915:Penicillin-Binding Proteins; D010458:Peptidyl Transferases; D006602:Hexosyltransferases; D002267:Muramoylpentapeptide Carboxypeptidase; D002506:Cephalexin