Contribution of capillary endothelium to the processes of pulmonary tissue rebuilding in the course of acute enzymatic lung injury.
Ultrastructural analysis was made of the pulmonary capillary endothelial cells, with special regard paid to their possible contribution to the processes of pulmonary tissue rebuilding initiated by intratracheal infusion of a proteolytic enzyme-papain. Experimental animals (male Wistar rats) were additionally given BCG vaccine in doses activating the alveolar macrophage system. The study was performed after 1 day, and 1, 4 and 12 weeks following papain administration. In the animals given BCG vaccine, ultrastructural exponents of endothelial cell activation were found. Cumulation of inflammatory cells, mainly monocytes, was observed in vascular lumen. Application of papain solution to animals caused a number of damaging and exudative changes, being most pronounced within vascular endothelium in animals simultaneously given BCG vaccine. In later periods, rebuilding and productive processes, accompanied by collagen cumulation in the interstitium of interalveolar septa, dominated. In the animals receiving BCG vaccine and papain, ultrastructural pictures indicated active contribution of endothelial cells to the processes of pulmonary tissue rebuilding initiated by intratracheal infusion of the proteolytic enzyme.
Sulkowski
S
S
Department of Pathological Anatomy, Medical Academy of Białystok.
Sulkowska
M
M
Musiatowicz
B
B
Giedrojć
J
J
Terlikowski
S
S
Famulski
W
W
D000818:Animals; D001500:BCG Vaccine / Q000494:pharmacology; D002196:Capillaries / Q000166:cytology; D004646:Emphysema / Q000139:chemically induced / Q000503:physiopathology*; D004730:Endothelium, Vascular / Q000502:physiology* / Q000648:ultrastructure; D005122:Exudates and Transudates; D007249:Inflammation; D008168:Lung / Q000098:blood supply* / Q000187:drug effects; D008262:Macrophage Activation / Q000187:drug effects; D016676:Macrophages, Alveolar / Q000187:drug effects; D008297:Male; D010206:Papain / Q000008:administration & dosage / Q000633:toxicity*; D051381:Rats; D017208:Rats, Wistar; D014945:Wound Healing / Q000502:physiology*
D001500:BCG Vaccine; D010206:Papain