Comparison of two methods in erythrocyte microrheology determination using glutaraldehyde-treated cells.
The change in human red blood cell microrheology in different glutaraldehyde concentrations (1.5, 3.0 and 5.0 x 10(-6) mol l-1) was studied. The method of millipore filtration was compared with the method of cation-osmotic hemolysis. The obtained results revealed that the prolongation of the erythrocyte filtration time correlated with the curve shifts found in cation-osmotic hemolysis. Contrary to the filtration method, significant differences between two lower concentrations of glutaraldehyde (1.5 and 3.0 x 10(-6) mol l-1) were found. Therefore, we conclude that the cation-osmotic hemolysis is more sensitive than the filtration method in determining the red blood cell deformability.
Mirossay
L
L
Department of Pharmacology, Medical Faculty, University P.J. Safarik, Kosice, Slovakia.
Mojzis
J
J
Jandoseková
M
M
Lukacín
S
S
Nicák
A
A
D000328:Adult; D001782:Blood Donors; D004907:Erythrocyte Deformability / Q000187:drug effects*; D004910:Erythrocyte Membrane / Q000187:drug effects*; D005374:Filtration; D005976:Glutaral / Q000494:pharmacology*; D006461:Hemolysis / Q000187:drug effects; D018056:Hemorheology / Q000379:methods*; D006801:Humans; D008832:Microchemistry; D008875:Middle Aged; D009997:Osmotic Pressure
D005976:Glutaral