Naturally occurring variants of human milk bile salt-stimulated lipase.
Analysis of milk samples from a number of lactating women revealed molecular variants of bile salt-stimulated lipase (BSSL) of both lower and higher molecular mass than that commonly occurring. In contrast to previous observations, we report on individuals having only a variant of lower mass, both one of lower and one of common mass, or both one of lower and one of higher mass of the lipase. From two individuals we purified the lower molecular mass BSSL variant and characterized it. The amount of lipase in the milk of these two individuals was considerably less than average (mean of 10 women with BSSL of the most common molecular mass). The BSSL variant of lower mass showed the same bile salt activation, pH dependency, temperature stability as those most commonly occurring. We could localize the difference in mass to the large O-glycosylated repeat sequence close to the C-terminus of the protein. With respect to all characteristics studied, the BSSL variant of higher mass was also similar to that most commonly ocurring. Again, the difference in mass could be localized to the repeat region of the protein. Hence, it appears as if the repeat region, normally carrying 16 repeats of 11 amino acids each, varies in size between individuals.
Strömqvist
M
M
Astra Hässle AB, Tvistevägen 48, Umeâ, S-907 36, Sweden. Mats.Stromqvist@hassle.se.astra.com
Hernell
O
O
Hansson
L
L
Lindgren
K
K
Skytt
A
A
Lundberg
L
L
Lidmer
A S
AS
Bläckberg
L
L
D000596:Amino Acids / Q000032:analysis; D000818:Animals; D001647:Bile Acids and Salts / Q000494:pharmacology; D015153:Blotting, Western; D004591:Electrophoresis, Polyacrylamide Gel; D004789:Enzyme Activation; D004795:Enzyme Stability; D005260:Female; D014644:Genetic Variation; D006031:Glycosylation; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007774:Lactation; D008049:Lipase / Q000737:chemistry* / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism; D008895:Milk, Human / Q000201:enzymology*; D008970:Molecular Weight; D009844:Oligosaccharides / Q000032:analysis / Q000737:chemistry; D011994:Recombinant Proteins / Q000737:chemistry; D012685:Sepharose / Q000031:analogs & derivatives / Q000378:metabolism; D002787:Sterol Esterase; D013696:Temperature
D000596:Amino Acids; D001647:Bile Acids and Salts; D009844:Oligosaccharides; D011994:Recombinant Proteins; C037405:heparin-sepharose; D012685:Sepharose; C054784:bile salt-stimulated lipase; D002787:Sterol Esterase; D008049:Lipase