title: High responsiveness of cytosolic free calcium concentration to angiotensin II in cultured pulmonary arterial myocytes from pulmonary hypertensive rats.
abstract: Pulmonary arterial myocytes were cultured from normotensive and pulmonary hypertensive rats. Microfluorimetry of Ca2+ signals in fluo-3-loaded single myocytes at day 7 of culture was performed by a laser-scanned confocal imaging system. The resting level of cytoplasmic Ca2+ concentration ([Ca2+]i) in vascular myocytes obtained from hypertensive rats was higher than that in cultured myocytes obtained from normotensive rats. Angiotensin II elevated [Ca2+]i in the vascular myocytes cultured from both normotensive and hypertensive rats. However, a rise of [Ca2+]i induced by angiotensin II in the vascular myocytes obtained from pulmonary hypertensive rats was higher than that obtained from normotensive rats. On the other hand, the response of [Ca2+]i to A23187 did not differ between the vascular myocytes cultured from normotensive and hypertensive rats. The present results suggest that the resting and angiotensin II-responsive levels of [Ca2+]i in pulmonary arterial myocytes cultured from pulmonary hypertensive rats are higher than those cultured from normotensive rats.
authors.affiliation: Department of Pharmacology, China Medical College, Shenyang.
authors.forename: Z
authors.initials: Z
authors.lastname: Li
authors.forename: Q
authors.initials: Q
authors.lastname: Zhang
authors.forename: S
authors.initials: S
authors.lastname: Zhao
authors.forename: M
authors.initials: M
authors.lastname: Wei
authors.forename: H
authors.initials: H
authors.lastname: Cong
authors.forename: H
authors.initials: H
authors.lastname: Ouda
authors.forename: K
authors.initials: K
authors.lastname: Odajima
authors.forename: H
authors.initials: H
authors.lastname: Takemura
mesh_terms: D000804:Angiotensin II / Q000494:pharmacology*; D000814:Aniline Compounds; D000818:Animals; D000001:Calcimycin / Q000494:pharmacology; D002118:Calcium / Q000032:analysis*; D002478:Cells, Cultured / Q000187:drug effects; D003600:Cytosol / Q000378:metabolism; D005456:Fluorescent Dyes; D006976:Hypertension, Pulmonary / Q000378:metabolism*; D009131:Muscle, Smooth, Vascular / Q000187:drug effects*; D011651:Pulmonary Artery / Q000187:drug effects* / Q000378:metabolism; D051381:Rats; D014662:Vasoconstrictor Agents / Q000494:pharmacology*; D014966:Xanthenes
chemical_list: D000814:Aniline Compounds; D005456:Fluorescent Dyes; D014662:Vasoconstrictor Agents; D014966:Xanthenes; D000804:Angiotensin II; C059715:Fluo-3; D000001:Calcimycin; D002118:Calcium