Effect of human hydrosalpinx fluid on murine embryo development and implantation.
OBJECTIVE
To determine the effect of hydrosalpinx fluid-containing medium on murine embryo development and implantation.


METHODS
The development of one-, two-, and four-cell mouse embryos in medium containing 5%, 10%, and 20% of human hydrosalpinx fluid was observed. Implantation rates of mouse embryos transferred into the uterine horn with hydrosalpinx fluid-containing media were determined.


METHODS
Private hospital-based fertility center and IVF program.


METHODS
Percentage of embryos continuing cell division and implantation rates after ET.


RESULTS
Hydrosalpinx fluid in culture medium affected embryo development in a dose-dependent fashion. The injection of hydrosalpinx fluid-containing medium into the uterine horn did not affect embryo implantation.


CONCLUSIONS
Hydrosalpinx fluid negatively affects murine embryo development, but its presence in the uterine horn at ET did not affect implantation.
Rawe
V J
VJ
Greater Baltimore Fertility Center, Department of Gynecology, Greater Baltimore Medical Center, Maryland, USA.
Liu
J
J
Shaffer
S
S
Compton
M G
MG
García
J E
JE
Katz
E
E
D000818:Animals; D001826:Body Fluids / Q000502:physiology*; D003470:Culture Media; D004305:Dose-Response Relationship, Drug; D010064:Embryo Implantation / Q000502:physiology; D004624:Embryo Transfer; D005314:Embryonic and Fetal Development / Q000502:physiology*; D005184:Fallopian Tube Diseases / Q000378:metabolism*; D005260:Female; D005307:Fertilization in Vitro; D006801:Humans; D007267:Injections; D051379:Mice / Q000196:embryology; D008815:Mice, Inbred Strains; D009994:Osmolar Concentration
D003470:Culture Media