title: Structure of the collagen-binding domain from a Staphylococcus aureus adhesin.
abstract: The crystal structure of the recombinant 19,000 M(r) binding domain from the Staphylococcus aureus collagen adhesin has been determined at 2 A resolution. The domain fold is a jelly-roll, composed of two antiparallel beta-sheets and two short alpha-helices. Triple-helical collagen model probes were used in a systematic docking search to identify the collagen-binding site. A groove on beta-sheet I exhibited the best surface complementarity to the collagen probes. This site partially overlaps with the peptide sequence previously shown to be critical for collagen binding. Recombinant proteins containing single amino acid mutations designed to disrupt the surface of the putative binding site exhibited significantly lower affinities for collagen. Here we present a structural perspective for the mode of collagen binding by a bacterial surface protein.
authors.affiliation: Center for Macromolecular Crystallography, University of Alabama at Birmingham 35294, USA.
authors.forename: J
authors.initials: J
authors.lastname: Symersky
authors.forename: J M
authors.initials: JM
authors.lastname: Patti
authors.forename: M
authors.initials: M
authors.lastname: Carson
authors.forename: K
authors.initials: K
authors.lastname: House-Pompeo
authors.forename: M
authors.initials: M
authors.lastname: Teale
authors.forename: D
authors.initials: D
authors.lastname: Moore
authors.forename: L
authors.initials: L
authors.lastname: Jin
authors.forename: A
authors.initials: A
authors.lastname: Schneider
authors.forename: L J
authors.initials: LJ
authors.lastname: DeLucas
authors.forename: M
authors.initials: M
authors.lastname: Höök
authors.forename: S V
authors.initials: SV
authors.lastname: Narayana
mesh_terms: D018829:Adhesins, Bacterial / Q000737:chemistry* / Q000378:metabolism*; D001665:Binding Sites; D003001:Cloning, Molecular; D003094:Collagen / Q000378:metabolism*; D018360:Crystallography, X-Ray; D004926:Escherichia coli; D008958:Models, Molecular; D008961:Models, Structural; D017510:Protein Folding; D021382:Protein Sorting Signals / Q000737:chemistry; D017433:Protein Structure, Secondary; D011994:Recombinant Proteins / Q000737:chemistry / Q000378:metabolism; D013211:Staphylococcus aureus / Q000378:metabolism
chemical_list: D018829:Adhesins, Bacterial; D021382:Protein Sorting Signals; D011994:Recombinant Proteins; D003094:Collagen