In vitro transcription systems from cultured cells and fat-body tissue of the silkworm, Bombyx mori.
This article describes the development of cell-free transcription systems from the cultured cells and fat body tissues of a Lepidpteran insect, the silkworm, Bombyx mori. Detailed protocols are provided for the culture of a B. mori cell line, rearing larvae, preparation of whole cell as well as nuclear extract and conditions for in vitro transcription of cloned plasma protein gene templates.
Mine
E
E
Department of Biology, Tokyo Metropolitan University, Japan.
Sakurai
H
H
Izumi
S
S
Tomino
S
S
D000818:Animals; D012831:Bombyx / Q000378:metabolism*; D002474:Cell-Free System; D002478:Cells, Cultured; D005216:Fat Body / Q000378:metabolism*; D005260:Female; D005821:Genetic Techniques; D007202:Indicators and Reagents; D010053:Ovary / Q000166:cytology / Q000378:metabolism; D014158:Transcription, Genetic
D007202:Indicators and Reagents