Microelectrode arrays for electrophysiological monitoring of hippocampal organotypic slice cultures.
A three-dimensional platinum (Pt) microelectrode array embedded on a micromachined silicon (Si) substrate (porosity of 13%, via hole diameter of 40 microns) has been developed. Electrodes are 35-micron wide and 20-microns high, spaced 200 microns apart and arranged in an elliptic geometry. Integrated within a microperfusion chamber, the devices were used for stimulation and recording experiments of hippocampal slice cultures over a period of several days.
Thiébaud
P
P
Institute of Microtechnology, University of Neuchâtel, Switzerland. pierrethiebaud@imt.unine.ch
de Rooij
N F
NF
Koudelka-Hep
M
M
Stoppini
L
L
D000818:Animals; D004553:Electric Conductivity; D004594:Electrophysiology / Q000295:instrumentation*; D004867:Equipment Design; D005071:Evoked Potentials; D006624:Hippocampus / Q000502:physiology*; D008839:Microelectrodes; D008991:Monitoring, Physiologic; D009924:Organ Culture Techniques / Q000295:instrumentation*; D010984:Platinum; D051381:Rats; D017208:Rats, Wistar
D010984:Platinum