Rapid changes of heteronuclear RNA for arginine vasopressin but not for corticotropin releasing hormone in response to acute corticosterone administration.
Corticotropin-releasing hormone and arginine vasopressin gene transcription were studied in the parvocellular cells of the hypothalamic paraventricular nucleus in response to both adrenalectomy and the injection of corticosterone. Following 6 days ADX, there was an increase in AVP mRNA, AVP hnRNA and CRH mRNA but not CRH hnRNA. After injection of corticosterone in ADX rats there was an extremely rapid reduction in AVP hnRNA which fell markedly within 15 min. AVP mRNA, however, remained raised throughout the 4 h. CRH hnRNA fell at 2 and 4 h, while CRH mRNA was only significantly reduced at the 4 h time point. Data demonstrate markedly different kinetics for the glucocorticoid regulation of the AVP and CRH genes in the hypothalamus.
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Dorothy Crowfoot Hodgkin Laboratories, University of Bristol, Department of Medicine, Bristol Royal Infirmary, UK.
Levy
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Lightman
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D000315:Adrenalectomy; D000818:Animals; D001127:Arginine Vasopressin / Q000096:biosynthesis*; D003345:Corticosterone / Q000097:blood / Q000494:pharmacology*; D003346:Corticotropin-Releasing Hormone / Q000096:biosynthesis*; D008297:Male; D010286:Paraventricular Hypothalamic Nucleus / Q000187:drug effects / Q000378:metabolism; D012332:RNA, Heterogeneous Nuclear / Q000096:biosynthesis / Q000378:metabolism*; D012333:RNA, Messenger / Q000096:biosynthesis / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley
D012332:RNA, Heterogeneous Nuclear; D012333:RNA, Messenger; D001127:Arginine Vasopressin; D003346:Corticotropin-Releasing Hormone; D003345:Corticosterone