title: 3-Hydroxybutyrate co-infused with noradrenaline decreases resulting plasma levels of noradrenaline in Wistar rats.
abstract: Pentobarbital-anaesthetized male Wistar rats were infused with 6microgkg-1min-1 of noradrenaline. The infusion was supplemented with 8.5 mgkg-1min-1 of D-3-hydroxybutyrate (3-OHB) for 15 min in order to determine its effect on the adrenergic response of the rat. Plasma levels of noradrenaline rose to a plateau of approximately 50 nmoll-1 with infusion. In the group infused with noradrenaline alone, noradrenaline levels were maintained for 1h. Supplementation with 3-OHB induced a decrease in plasma noradrenaline level that was inversely correlated with 3-OHB level. Aortic and interscapular brown adipose tissue temperatures increased with noradrenaline infusion, but the rise was arrested by 3-OHB; replacing 3-OHB with glucose had no effect. Infusion of saline, glucose or 3-OHB in the absence of noradrenaline did not induce a rise in temperature in either tissue. Blood 3-OHB concentration increased to 1.2 mmoll-1 during 3-OHB infusion, decreasing rapidly at the end of infusion. Blood glucose levels increased with noradrenaline infusion; the presence of high 3-OHB levels decreased glucose concentration. The effects observed were transient and dependent on 3-OHB concentration; these effects may help explain most of the other effects of noradrenaline described here. The role of 3-OHB as a regulator of adrenergic responses seems to be part of a complex fail-safe mechanism which prevents wasting.
authors.affiliation: Departament de Bioquímica i Biologia Molecular, Facultat de Biologia, Universitat de Barcelona, Barcelona, Spain.
authors.forename: N
authors.initials: N
authors.lastname: Cañas
authors.forename: D
authors.initials: D
authors.lastname: Sanchis
authors.forename: G
authors.initials: G
authors.lastname: Gómez
authors.forename: J M
authors.initials: JM
authors.lastname: Casanovas
authors.forename: F
authors.initials: F
authors.lastname: Artigas
authors.forename: J A
authors.initials: JA
authors.lastname: Fernández-López
authors.forename: X
authors.initials: X
authors.lastname: Remesar
authors.forename: X
authors.initials: X
authors.lastname: Alemany
mesh_terms: D020155:3-Hydroxybutyric Acid; D000818:Animals; D001831:Body Temperature; D006885:Hydroxybutyrates / Q000008:administration & dosage*; D007262:Infusions, Intravenous; D008297:Male; D009638:Norepinephrine / Q000008:administration & dosage / Q000097:blood*; D051381:Rats; D017208:Rats, Wistar
chemical_list: D006885:Hydroxybutyrates; D020155:3-Hydroxybutyric Acid; D009638:Norepinephrine