Effect of polymers on enhanced chemiluminescent assays for peroxidase and peroxidase labels.
Hydroxypropyl methylcellulose, hydroxyethyl cellulose, and hydroxybutyl methylcellulose stabilized light emission in a boronic acid-enhanced chemiluminescent assay for horseradish peroxidase. The stabilization of light emission was concentration-dependent and more effective with substituted boronic acid enhancers (e.g. 4-iodophenylboronic acid) than with substituted phenol enhancers (e.g. 4-iodophenol). Hydroxybutyl methylcellulose improved the linearity of the dose-response curve in a peroxidase-based antioxidant assay and stabilized light emission post-consumption of the antioxidant (Trolox). This polymer had no effect on the signal from a peroxidase label immobilized on a membrane (dot blot) or on the inside surface of a microwell in an enzyme immunoassay for thyrotropin.
Ji
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Department of Pathology and Laboratory Medicine, University of Pennsylvania Medical Center, Philadelphia 19104, USA.
Kricka
L J
LJ
D000975:Antioxidants; D002839:Chromans; D005069:Evaluation Studies as Topic; D006735:Horseradish Peroxidase / Q000032:analysis; D007124:Immunoenzyme Techniques; D007202:Indicators and Reagents; D008163:Luminescent Measurements; D010544:Peroxidases / Q000032:analysis*; D011108:Polymers; D013972:Thyrotropin / Q000032:analysis
D000975:Antioxidants; D002839:Chromans; D007202:Indicators and Reagents; D011108:Polymers; D013972:Thyrotropin; D006735:Horseradish Peroxidase; D010544:Peroxidases; C010643:6-hydroxy-2,5,7,8-tetramethylchroman-2-carboxylic acid