Characterization of Phaseolus vulgaris cDNA clones responsive to water deficit: identification of a novel late embryogenesis abundant-like protein.
Six cDNA clones from Phaseolus vulgaris, whose expression is induced by water deficit and ABA treatment (rsP cDNAs) were identified and characterized. The sequence analyses of the isolated clones suggest that they encode two types of late-embryogenesis abundant (LEA) proteins, a class-1 cytoplasmic low-molecular-weight heat shock protein (lmw-HSP), a lipid transfer protein (LTP), and two different proline-rich proteins (PRP). One of the putative LEA proteins identified corresponds to a novel 9.3 kDa LEA-like protein. During the plant response to a mild water deficit (psi w = -0.35 MPa) all genes identified present a maximal expression at around 16 or 24 h of treatment, followed by a decline in expression levels. Rehydration experiments revealed that those genes encoding PRPs and LTP transiently re-induce or maintain their expression when water is added to the soil after a dehydration period. This is not the case for the lea genes whose transcripts rapidly decrease, reaching basal levels a few hours after rehydration (4 h). Under water deficit and ABA treatments, the highest levels of expression for most of the genes occur in the root, excluding the ltp gene whose maximum expression levels are found in the aerial regions of the plant. This indicates that for these genes, both water deficit and ABA-dependent expression are under organ-specific control. The data presented here support the importance of these proteins during the plant response to water deficit.
Colmenero-Flores
J M
JM
Departmento de Biologia Molecular de Plantas, Universidad Nacional Autónoma de México, México.
Campos
F
F
Garciarrubio
A
A
Covarrubias
A A
AA
D000040:Abscisic Acid / Q000494:pharmacology; D000595:Amino Acid Sequence; D052179:Antigens, Plant; D001483:Base Sequence; D002352:Carrier Proteins / Q000235:genetics; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics*; D018744:DNA, Plant / Q000235:genetics*; D003681:Dehydration; D007887:Fabaceae / Q000196:embryology / Q000235:genetics*; D018506:Gene Expression Regulation, Plant / Q000187:drug effects / Q000502:physiology*; D018525:Germination; D006360:Heat-Shock Proteins / Q000235:genetics; D008969:Molecular Sequence Data; D016366:Open Reading Frames / Q000235:genetics; D010455:Peptides / Q000235:genetics; D010937:Plant Growth Regulators / Q000494:pharmacology; D010940:Plant Proteins / Q000737:chemistry / Q000235:genetics*; D018517:Plant Roots / Q000737:chemistry; D010946:Plants, Medicinal; D055232:Proline-Rich Protein Domains; D017433:Protein Structure, Secondary; D012333:RNA, Messenger / Q000032:analysis; D018749:RNA, Plant / Q000032:analysis; D012639:Seeds / Q000737:chemistry; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid
D052179:Antigens, Plant; D002352:Carrier Proteins; D018076:DNA, Complementary; D018744:DNA, Plant; D006360:Heat-Shock Proteins; D010455:Peptides; D010937:Plant Growth Regulators; D010940:Plant Proteins; D012333:RNA, Messenger; D018749:RNA, Plant; C066246:late embryogenesis abundant protein, plant; C511920:lipid transfer proteins, plant; D000040:Abscisic Acid