Biological activity C-X-C and C-C chemokines on leukocyte subpopulations in human whole blood.
We have described an assay that monitors the activating effects of a variety of chemokines on leukocyte subsets in human whole blood. This procedure has the following advantages: (1) minimal manipulation of the cells, (2) maintenance of more physiological conditions, and (3) simultaneous monitoring of the responses of monocytes, neutrophils, and eosinophils.
Conklyn
M J
MJ
Department of Cancer, Immunology and Infectious Diseases, Pfizer, Inc., Groton, Connecticut 06340, USA.
Showell
H J
HJ
D000906:Antibodies / Q000276:immunology; D019742:Chemokines, CC / Q000494:pharmacology*; D019743:Chemokines, CXC / Q000494:pharmacology*; D004804:Eosinophils / Q000276:immunology; D005434:Flow Cytometry; D016650:Fluorescein-5-isothiocyanate / Q000378:metabolism; D006801:Humans; D007962:Leukocytes / Q000145:classification / Q000276:immunology*; D008070:Lipopolysaccharides / Q000494:pharmacology; D016177:Macrophage-1 Antigen / Q000097:blood*; D009000:Monocytes / Q000276:immunology; D009504:Neutrophils / Q000276:immunology; D015854:Up-Regulation
D000906:Antibodies; D019742:Chemokines, CC; D019743:Chemokines, CXC; D008070:Lipopolysaccharides; D016177:Macrophage-1 Antigen; D016650:Fluorescein-5-isothiocyanate