IL-7 induces proliferation, variable cytokine-producing ability and IL-2 responsiveness in naive CD4+ T-cells from human cord blood.
We investigated the effects of IL-7 on the proliferation and acquisition of cytokine-producing ability of naive CD4+ T-cells from human cord blood. Naive CD4+CD45RA+ T-cells from human cord blood expressed CDw127 (IL-7R) at higher levels than adult CD4+ CD45RA+ T-cells and produced IL-2 and a small amount of IFN-gamma upon stimulation with PMA and ionomycin. IL-7 induced IL-2-independent proliferation and both Th1- and Th2-type cytokine-producing abilities in cord blood CD4+CD45RA+ T-cells without stimulation via TCR. These results suggest that this IL-7-induced antigen-independent activation mechanism could contribute to maintaining the clonal size of naive T-cells with the potential to differentiate into either Th1 or Th2 cells at the sites of IL-7-expression.
Fukui
T
T
Department of Pediatrics, Faculty of Medicine, Kyoto University, Japan.
Katamura
K
K
Abe
N
N
Kiyomasu
T
T
Iio
J
J
Ueno
H
H
Mayumi
M
M
Furusho
K
K
D000328:Adult; D015703:Antigens, CD / Q000032:analysis; D015496:CD4-Positive T-Lymphocytes / Q000187:drug effects / Q000378:metabolism*; D002455:Cell Division / Q000187:drug effects; D002469:Cell Separation; D002478:Cells, Cultured; D016207:Cytokines / Q000096:biosynthesis*; D005312:Fetal Blood; D005434:Flow Cytometry; D006801:Humans; D007371:Interferon-gamma / Q000096:biosynthesis; D007376:Interleukin-2 / Q000097:blood*; D015847:Interleukin-4 / Q000096:biosynthesis; D015851:Interleukin-7 / Q000378:metabolism / Q000494:pharmacology*; D017493:Leukocyte Common Antigens / Q000032:analysis; D018123:Receptors, Interleukin / Q000032:analysis; D015375:Receptors, Interleukin-2 / Q000096:biosynthesis; D020395:Receptors, Interleukin-7
D015703:Antigens, CD; D016207:Cytokines; D007376:Interleukin-2; D015851:Interleukin-7; D018123:Receptors, Interleukin; D015375:Receptors, Interleukin-2; D020395:Receptors, Interleukin-7; D015847:Interleukin-4; D007371:Interferon-gamma; D017493:Leukocyte Common Antigens