title: Synthesis of oligonucleotides labelled with 2,4-dinitrophenyl groups at thymidine sites.
abstract: A phosphoramidite has been produced for labelling oligonucleotides with DNP groups at thymidine sites during solid-phase synthesis. The dinitrophenylamino group is attached via a caproamidopropargyl group to the 5-position of uracil. A related DNP-labelling phosphoramidite has been synthesised where the propargyl group is replaced by propyl. Both phosphoramidites have been used to synthesises DNP-labelled oligonucleotides. A related DNP-labelled deoxyuridine triphosphate has also been synthesised. DNP labelled oligonucleotide probes are valuable in diagnostic applications for the antibody-based detection of DNA and RNA.
authors.affiliation: Cruachem Ltd. Glasgow, Scotland, U.K.
authors.forename: A
authors.initials: A
authors.lastname: Davison
authors.forename: J
authors.initials: J
authors.lastname: McClean
authors.forename: M V
authors.initials: MV
authors.lastname: Rao
authors.forename: T
authors.initials: T
authors.lastname: Brown
mesh_terms: D002851:Chromatography, High Pressure Liquid; D004136:Dinitrobenzenes / Q000737:chemistry*; D008956:Models, Chemical; D009841:Oligonucleotides / Q000138:chemical synthesis*; D013936:Thymidine / Q000737:chemistry*
chemical_list: D004136:Dinitrobenzenes; D009841:Oligonucleotides; D013936:Thymidine