SpsA, a novel pneumococcal surface protein with specific binding to secretory immunoglobulin A and secretory component.
The interaction of pathogenic bacteria with host serum and matrix proteins is a common strategy to enhance their virulence. Streptococcus pneumoniae colonizes the human upper respiratory tract in healthy individuals and is also able to cause invasive diseases. Here, we describe a novel pneumococcal surface protein, SpsA, capable of binding specifically to human secretory immunoglobulin A (SIgA). The dissociation constant of SIgA binding to SpsA was 9.3 x 10(-9) M. Free secretory component (SC) also binds to S. pneumoniae, whereas serum IgA does not, suggesting that pneumococcal binding to SIgA is mediated by the SC. To our knowledge, this is the first defined interaction of SC with a prokaryotic protein. The spsA gene encodes a polypeptide of 523 amino acids with a predicted molecular mass of 59 151 Da. The SIgA- or SC-binding domain is located in the N-terminal part of SpsA and exhibits no significant homology to any other proteins. The purified SIgA-binding domain of SpsA could completely inhibit the binding of SIgA to pneumococci. SpsA was expressed by 73% of the tested S. pneumoniae isolates and was substantially conserved between different serotypes. The interaction between S. pneumoniae and SC via SpsA represents a novel biological interaction that might increase virulence by the impairment of bacterial clearance.
Hammerschmidt
S
S
Department of Microbial Pathogenesis, GBF-National Research Centre for Biotechnology, Braunschweig, Germany.
Talay
S R
SR
Brandtzaeg
P
P
Chhatwal
G S
GS
D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000235:genetics / Q000276:immunology*; D001483:Base Sequence; D001667:Binding, Competitive; D015153:Blotting, Western; D003001:Cloning, Molecular; D004269:DNA, Bacterial; D005798:Genes, Bacterial; D014644:Genetic Variation; D006801:Humans; D007071:Immunoglobulin A, Secretory / Q000276:immunology*; D008969:Molecular Sequence Data; D012635:Secretory Component / Q000276:immunology*; D017386:Sequence Homology, Amino Acid; D012703:Serotyping; D013296:Streptococcus pneumoniae / Q000235:genetics / Q000276:immunology* / Q000378:metabolism
D001426:Bacterial Proteins; D004269:DNA, Bacterial; D007071:Immunoglobulin A, Secretory; D012635:Secretory Component; C110344:SpsA protein, Streptococcus pneumoniae; C051592:pneumococcal surface protein A