Enhanced growth by ectopic expression of growth hormone releasing hormone using an injectable myogenic vector.
Ectopic expression of a truncated growth hormone-releasing hormone (GHRH) from muscle tissues by a myogenic plasmid DNA vector directed by the skeletal alpha-actin promoter, pSK-GHRH, results in growth hormone (GH) secretion. Skeletal muscle-secreted GHRH is biologically active. The application of conditioned media harvested from pSK-GHRH transfected muscle cells to cultured pig primary anterior pituitary cells elicits GH release. A single intramuscular injection of 100 micrograms pSK-GHRH DNA elevates serum GH levels threefold to fourfold for up to 2 weeks, enhancing liver IGF-1 gene expression and increasing body weight approximately 10%.
Draghia-Akli
R
R
Department of Cell Biology, Baylor College of Medicine, Houston, TX 77030, USA.
Li
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X
Schwartz
R J
RJ
D000818:Animals; D002478:Cells, Cultured; D017077:Culture Media, Conditioned; D004274:DNA, Recombinant; D005822:Genetic Vectors; D013007:Growth Hormone-Releasing Hormone / Q000008:administration & dosage / Q000235:genetics / Q000494:pharmacology*; D006801:Humans; D007334:Insulin-Like Growth Factor I / Q000235:genetics; D051379:Mice; D008810:Mice, Inbred C57BL; D018482:Muscle, Skeletal / Q000378:metabolism*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D011994:Recombinant Proteins / Q000008:administration & dosage / Q000235:genetics / Q000494:pharmacology; D013552:Swine
D017077:Culture Media, Conditioned; D004274:DNA, Recombinant; D012333:RNA, Messenger; D011994:Recombinant Proteins; D007334:Insulin-Like Growth Factor I; D013007:Growth Hormone-Releasing Hormone