Map-based cloning of the tomato genomic region that spans the Sw-5 tospovirus resistance gene in tomato.
Two yeast artificial chromosomes (YACs) containing genomic DNA from tomato have been isolated using CT220, an RFLP marker which is tightly linked to the tomato spotted wilt virus resistance gene, Sw-5. High-resolution mapping of the YAC ends and internal YAC probes demonstrated that one of the YAC clones, TY257 (400 kb), spans Sw-5. By chromosome walking in a cosmid library, the position of Sw-5 has been delimited within the YAC to a maximal chromosomal segment of 100 kb, spanned by nine overlapping cosmid clones.
Brommonschenkel
S H
SH
Department of Plant Breeding, Cornell University, Ithaca, NY 14850, USA.
Tanksley
S D
SD
D002874:Chromosome Mapping; D018244:Chromosomes, Artificial, Yeast / Q000235:genetics; D003001:Cloning, Molecular; D003360:Cosmids; D018744:DNA, Plant / Q000235:genetics; D015723:Gene Library; D017343:Genes, Plant; D007113:Immunity, Innate / Q000235:genetics*; D018551:Solanum lycopersicum / Q000235:genetics* / Q000821:virology; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length; D011995:Recombination, Genetic; D017865:Tospovirus / Q000235:genetics / Q000472:pathogenicity
D018744:DNA, Plant