Short report: Leishmania DNA in Phlebotomus and Sergentomyia species during a kala-azar epidemic.
The presence of Leishmania donovani DNA in sand flies caught in Indian kala-azar patients' dwellings during the epidemic of 1990-1992 was studied using the polymerase chain reaction (PCR). Amplification of miniexon-derived RNA genes and gpG3 mRNA was achieved in single Phlebotomus argentipes, P. papatasi, and Sergentomyia babu flies. The data suggest the possible involvement of multiple sandfly species in kala-azar transmission.
Mukherjee
S
S
Indian Institute of Chemical Biology, Calcutta.
Hassan
M Q
MQ
Ghosh
A
A
Ghosh
K N
KN
Bhattacharya
A
A
Adhya
S
S
D000818:Animals; D016054:DNA, Protozoan / Q000302:isolation & purification*; D004196:Disease Outbreaks; D005260:Female; D006801:Humans; D007893:Leishmania donovani / Q000235:genetics*; D007898:Leishmaniasis, Visceral / Q000453:epidemiology / Q000635:transmission*; D010691:Phlebotomus / Q000235:genetics / Q000469:parasitology; D016133:Polymerase Chain Reaction; D011576:Psychodidae / Q000235:genetics* / Q000469:parasitology; D013045:Species Specificity
D016054:DNA, Protozoan