title: 1H NMR spectra of normal urines: reference ranges of the major metabolites.
abstract: Serial urine samples from 50 normal subjects were studied by 1H NMR spectroscopy operating at 300 MHz. Analyses of the spectra have shown the presence of the following metabolites in 100% of the normal subjects: Creatinine, lactate, alanine, citrate, dimethylamine, trimethylamine-N-oxide, glycine and hippurate. Other analytes, such as creatine, valine, betaine, leucine and isoleucine, were sometimes found. All metabolites were quantified on the basis of peak heights and were expressed as mmol/mol of creatinine. The study of metabolic profiles in serial samples allowed us to evaluate intra-individual variability and physiological changes due to feeding. The aim of our report is to define standard conditions for this analytical technique and to calculate confidence intervals for the major metabolites in normal urine samples, such as preliminary and mandatory stages for clinical diagnostic 1H NMR utilization.
authors.affiliation: Institute di Chimica e Chimica Clinica, Policlinico Universitario A. Gemelli, Università Cattolica del Sacro Cuore, Rome, Italy.
authors.forename: C
authors.initials: C
authors.lastname: Zuppi
authors.forename: I
authors.initials: I
authors.lastname: Messana
authors.forename: F
authors.initials: F
authors.lastname: Forni
authors.forename: C
authors.initials: C
authors.lastname: Rossi
authors.forename: L
authors.initials: L
authors.lastname: Pennacchietti
authors.forename: F
authors.initials: F
authors.lastname: Ferrari
authors.forename: B
authors.initials: B
authors.lastname: Giardina
mesh_terms: D000328:Adult; D004435:Eating; D005260:Female; D006801:Humans; D006863:Hydrogen-Ion Concentration; D016014:Linear Models; D009682:Magnetic Resonance Spectroscopy / Q000379:methods*; D008297:Male; D008875:Middle Aged; D011522:Protons; D012016:Reference Values; D015203:Reproducibility of Results; D012737:Sex Factors; D014556:Urine / Q000737:chemistry*
chemical_list: D011522:Protons