Expression of the human erythrocyte glucose transporter Glut1 in cutaneous neoplasia.
BACKGROUND
The increased glucose uptake seen in cancer cells correlates with the expression of human erythrocyte glucose transporter (Glut1) protein in certain human malignancies.


OBJECTIVE
Our purpose was to determine Glut1 expression in cutaneous neoplasms.


METHODS
A polyclonal anti-Glut1 antibody (MYM) and a standard ABC immunoperoxidase technique were used to determine Glut1 expression in invasive squamous cell carcinomas (SCCs), SCC in situ, basal cell carcinomas (BCCs), melanomas, actinic keratoses (AKs), seborrheic keratoses, common acquired nevi, and scars with regenerative epidermal hyperplasia.


RESULTS
All of the cases of SCC in situ, 14 of 15 (93%) of the SCC, and 13 of 15 AKs (87%) showed intense membranous staining for Glut1. Glut1 staining was present in the epidermis of 8 of 15 scars (53%) but was not detected in any BCC, even in areas of focal keratinization and squamous metaplasia. Glut1 reactivity was absent in the melanomas and seborrheic keratoses.


CONCLUSIONS
Glut1 expression in a cutaneous lesion strongly suggests a proliferative lesion of the squamous cell type.
Baer
S C
SC
Department of Dermatology, Baylor College of Medicine, Houston, Texas, USA.
Casaubon
L
L
Younes
M
M
D000906:Antibodies; D002278:Carcinoma in Situ / Q000378:metabolism / Q000473:pathology; D002280:Basal Cell Carcinoma / Q000378:metabolism / Q000473:pathology; D002294:Carcinoma, Squamous Cell / Q000378:metabolism / Q000473:pathology; D002455:Cell Division; D002921:Cicatrix / Q000378:metabolism / Q000473:pathology; D012628:Dermatitis, Seborrheic / Q000378:metabolism / Q000473:pathology; D004817:Epidermis / Q000378:metabolism / Q000473:pathology; D004847:Epithelial Cells / Q000378:metabolism / Q000473:pathology; D015972:Gene Expression Regulation, Neoplastic; D005947:Glucose / Q000378:metabolism; D051272:Glucose Transporter Type 1; D006801:Humans; D006965:Hyperplasia; D007124:Immunoenzyme Techniques; D007633:Keratins / Q000032:analysis; D007642:Keratosis / Q000378:metabolism / Q000473:pathology; D008545:Melanoma / Q000378:metabolism / Q000473:pathology; D009004:Monosaccharide Transport Proteins / Q000032:analysis* / Q000235:genetics; D009361:Neoplasm Invasiveness; D009506:Nevus / Q000378:metabolism / Q000473:pathology; D012038:Regeneration; D012878:Skin Neoplasms / Q000378:metabolism / Q000473:pathology*
D000906:Antibodies; D051272:Glucose Transporter Type 1; D009004:Monosaccharide Transport Proteins; C495677:SLC2A1 protein, human; D007633:Keratins; D005947:Glucose