Fucoidin, a potent inhibitor of L-selectin function, reduces contact hypersensitivity reaction in mice.
In order to investigate the role of L-selectin (CD62L) in delayed-type hypersensitivity (DTH) reaction, effect of fucoidin, a potent inhibitor of CD62L function, was examined in a model of mouse contact hypersensitivity reaction. Intravenous injection of fucoidin to sensitized mice just before hapten challenge resulted in a significant and dose-dependent reduction of the ear swelling in contact hypersensitivity. The ear swelling caused by the hapten challenge was also inhibited when fucoidin was administered at the sensitization phase. Histological analyses of the ear sections revealed that the fucoidin-induced suppression of contact hypersensitivity reflected a marked inhibition of the ear edema and the leukocyte infiltration. The activity of fucoidin was specific in that its related saccharides exerted little effect on the reaction. These results suggest that CD62L may play an important role in both afferent and efferent phases of cutaneous DTH reaction. Since DTH response is one of the most significant features of several chronic inflammatory diseases, our data also show that blocking of CD62L function may be beneficial for the treatment of these diseases in humans.
Nasu
T
T
Suntory Institute for Biomedical Research, Osaka, Japan.
Fukuda
Y
Y
Nagahira
K
K
Kawashima
H
H
Noguchi
C
C
Nakanishi
T
T
D000818:Animals; D003877:Dermatitis, Contact / Q000503:physiopathology*; D004195:Disease Models, Animal; D005260:Female; D019041:L-Selectin / Q000502:physiology*; D007962:Leukocytes / Q000187:drug effects; D051379:Mice; D008807:Mice, Inbred BALB C; D011134:Polysaccharides / Q000494:pharmacology*
D011134:Polysaccharides; D019041:L-Selectin; C007789:fucoidan