Transfer of small YACs to E. coli as large circular plasmids.
We have designed a YAC circularization vector, pCIRC3, allowing enrichment of the YAC DNA by exonuclease digestion of the linear yeast chromosomes. Due to the presence of P1 replicon sequences in this vector, the circular YACs would replicate as PACs in Escherischia coli.
Frengen
E
E
Biotechnology Centre of Oslo, University of Oslo, Norway. efrengen@biotek.uio.no
Wu
C
C
de Jong
P J
PJ
D018244:Chromosomes, Artificial, Yeast; D004270:DNA, Circular; D004926:Escherichia coli / Q000235:genetics*; D010957:Plasmids
D004270:DNA, Circular