title: Effects of time of storage, albumin, and osmolality changes on outflow facility (C) of bovine anterior segment in vitro.
abstract: OBJECTIVE
  To analyze the influence of time of storage, the presence of albumin at physiological concentrations, and the perfusion with anisosmotic media on the aqueous humor outflow facility (C) of isolated bovine anterior segments (AS).
  
  
  METHODS
  Anterior segments dissected from cow eyes were perfused at a constant pressure of 10 mm Hg with Dulbecco's modified Eagle's medium (DMEM; osmolality 300 mOsm/kg), with hyposmotic media (150, 210, and 270 mOsm/kg), or with hyperosmotic media (360, 420, and 480 mOsm/kg). Outflow facility was calculated every 5 seconds as the ratio between average inflow from the reservoir (in microliters per minute) and the perfusion pressure (in millimeters of mercury). Three groups were studied: a 0-hour group, with AS perfused with DMEM 1 to 3 hours after enucleation; a 0-hour alb-group, with AS perfused with DMEM plus 0.1 mg/ml albumin 1 to 3 hours after enucleation; and a 24-hour group, with AS perfused after storage for 24 hours in DMEM. In the 0-hour groups, perfusion with increasingly hyposmotic or hyperosmotic media was also made in 30-minute steps, followed by a return to isosmotic medium for 90 minutes.
  
  
  RESULTS
  Perfusion of AS with DMEM for 9 hours caused a progressive increase in C that was statistically significant at 225 minutes in the 0-hour group perfused with DMEM and at 195 minutes in the 24-hour group perfused with DMEM. The 0-hour alb-group perfused with DMEM did not show changes in C throughout the 9-hour perfusion period. Perfusion with increasingly hyposmotic media induced a progressive decrease in C that did not recover on return to isotonic medium. Hyperosmotic media caused a progressive increase in C that returned to control values when isotonic medium was again perfused.
  
  
  CONCLUSIONS
  Preservation of tissue for C measurements is best achieved with short storage time (1 to 3 hours). Physiological concentrations of albumin (0.1 mg/ml) prevent development of washout, suggesting that albumin or an albumin-bound factor in aqueous humor may play a role in the maintenance of outflow resistance. Outflow facility also may be influenced by volume changes in the trabecular meshwork.
authors.affiliation: Neurophysiology Laboratory, Faculty of Medicina-Fundació CLINIC, University of Barcelona, Spain.
authors.forename: A
authors.initials: A
authors.lastname: Gual
authors.forename: A
authors.initials: A
authors.lastname: Llobet
authors.forename: R
authors.initials: R
authors.lastname: Gilabert
authors.forename: M
authors.initials: M
authors.lastname: Borrás
authors.forename: J
authors.initials: J
authors.lastname: Palés
authors.forename: M V
authors.initials: MV
authors.lastname: Bergamini
authors.forename: C
authors.initials: C
authors.lastname: Belmonte
mesh_terms: D000818:Animals; D000869:Anterior Eye Segment / Q000502:physiology*; D001082:Aqueous Humor / Q000378:metabolism*; D002417:Cattle; D006982:Hypertonic Solutions / Q000008:administration & dosage; D007038:Hypotonic Solutions / Q000008:administration & dosage; D009926:Organ Preservation; D009994:Osmolar Concentration; D010477:Perfusion; D012710:Serum Albumin, Bovine / Q000008:administration & dosage*; D013997:Time Factors; D014129:Trabecular Meshwork / Q000502:physiology
chemical_list: D006982:Hypertonic Solutions; D007038:Hypotonic Solutions; D012710:Serum Albumin, Bovine