Role of glutathione and nitric oxide in the energy metabolism of rat liver mitochondria.
Previous studies have suggested that nitric oxide (NO) inhibited mitochondrial respiration. NO and/or its intermediate(s) react with various molecules, such as hemeproteins and free SH groups. The inhibitory effect of NO on mitochondrial respiration was decreased by exogenously added glutathione (GSH). However, a decrease of intramitochondrial GSH by pretreating animals with L-buthionine sulfoximine had no appreciable effect on the inhibitory effect of isolated mitochondria. Furthermore, the effect of NO was not affected by depleting free SH residues in mitochondria by N-ethylmaleimide. These results suggest that cytosolic but not intramitochondrial GSH might be an important factor that determines the NO-dependent regulation of mitochondrial energy metabolism.
Nishikawa
M
M
Department of Biochemistry, Osaka City University Medical School, Japan.
Sato
E F
EF
Kuroki
T
T
Inoue
M
M
D000818:Animals; D019328:Buthionine Sulfoximine / Q000494:pharmacology; D019069:Cell Respiration / Q000187:drug effects; D004734:Energy Metabolism; D005033:Ethylmaleimide / Q000494:pharmacology; D005978:Glutathione / Q000494:pharmacology / Q000502:physiology*; D008297:Male; D008930:Mitochondria, Liver / Q000378:metabolism*; D009569:Nitric Oxide / Q000494:pharmacology / Q000502:physiology*; D010101:Oxygen Consumption; D051381:Rats; D017208:Rats, Wistar; D013438:Sulfhydryl Compounds / Q000378:metabolism / Q000494:pharmacology
D013438:Sulfhydryl Compounds; D009569:Nitric Oxide; D019328:Buthionine Sulfoximine; D005978:Glutathione; D005033:Ethylmaleimide