Effect of bovine herpes virus-1, bluetongue virus and akabane virus on the in vitro development of bovine embryos.
Bovine oocytes enclosed by follicular epithelial (FE) cells were exposed to bovine herpes virus-1 (BHV-1), bluetongue virus (BTV) and akabane virus (AV), matured in culture for 24 h and then in vitro fertilized. In the BHV-1-exposed group, BHV-1 was isolated from medium 3 days after in vitro fertilization at a high titer, and a severe cytopathic effect was observed in the FE cells. Oocytes in the BHV-1-exposed group developed to the 2-cell to 8-cell stage, but failed to develop into blastocysts. In the BTV-exposed group, BTV replication was observed in the FE cells during in vitro embryo development into blastocysts. In the AV-exposed group, no distinct AV replication was detected in FE cells during in vitro embryo development. These results indicate that these viruses failed to infect zona-intact oocytes or embryos during in vitro maturation and culture.
Tsuboi
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T
Microbiological Diagnosis Laboratory, National Institute of Animal Health, Ibaraki, Japan.
Imada
T
T
D000818:Animals; D001755:Blastocyst / Q000502:physiology / Q000821:virology; D001820:Bluetongue virus / Q000472:pathogenicity* / Q000502:physiology; D002043:Bunyaviridae / Q000472:pathogenicity* / Q000502:physiology; D002417:Cattle; D002460:Cell Line; D005314:Embryonic and Fetal Development / Q000502:physiology*; D005260:Female; D005307:Fertilization in Vitro / Q000662:veterinary*; D007242:Herpesvirus 1, Bovine / Q000472:pathogenicity* / Q000502:physiology; D007315:Insemination, Artificial; D007668:Kidney; D008297:Male; D009865:Oocytes / Q000166:cytology / Q000502:physiology / Q000821:virology; D006080:Ovarian Follicle; D014779:Virus Replication