Metabotropic glutamate receptor agonists evoke calcium waves in isolated Müller cells.
Glutamate is the most prominent excitatory neurotransmitter in the retina and brain. It has become clear that the physiology of many glial cells, including retinal Müller cells, is modified by a host of neurotransmitters, including glutamate. The experiments presented here demonstrate that Müller cells isolated from the tiger salamander retina have metabotropic glutamate receptors that, when activated, lead to the release of calcium ions (Ca2+) from intracellular stores. The Ca2+-sensitive fluorescent dye, Fura-2, and video imaging microscopy were used to monitor changes in cytosolic calcium ion concentration ([Ca2+]i) evoked by glutamate (30-50 microM), (1S,3R)-ACPD (50-200 microM), quisqualate (10-50 microM), and L-AP4 (5-100 microM). Bath application of each of these metabotropic receptor agonists in the absence of extracellular Ca2+ resulted in an increase in [Ca2+]i that often began in the distal end of the cell and occurred later in the endfoot. This wavelike increase in [Ca2+]i is reminiscent of the Ca2+ waves evoked in these cells by other Ca2+ releasing agents such as ryanodine and caffeine. Extracellular application ofATP also evoked increases in [Ca2+] in Müller cells. The presence on Müller cells of receptors for retinal neurotransmitters, such as glutamate and ATP, demonstrates that these glial cells can respond to changes in the retinal extracellular environment and hence neuronal activity. Since Müller cells span almost all layers of the retina, they are likely to be exposed to most retinal neurotransmitters. The Ca2+ waves evoked in Müller cells by neurotransmitters could represent a form of signaling from the outer retinal layers to the inner ones.
Keirstead
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SA
Department of Ophthalmology, University of Minnesota, Minneapolis 55455-3007, USA. sakeir@maroon.tc.umn.edu
Miller
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RF
D000255:Adenosine Triphosphate / Q000378:metabolism; D000557:Ambystoma; D000613:Aminobutyrates / Q000494:pharmacology; D000818:Animals; D015220:Calcium Channels / Q000378:metabolism*; D003515:Cycloleucine / Q000031:analogs & derivatives / Q000494:pharmacology; D018690:Excitatory Amino Acid Agonists / Q000494:pharmacology; D005456:Fluorescent Dyes; D006651:Histocytochemistry; D066298:In Vitro Techniques; D009457:Neuroglia / Q000378:metabolism*; D018696:Neuroprotective Agents / Q000494:pharmacology; D016318:Quisqualic Acid / Q000494:pharmacology; D018094:Receptors, Metabotropic Glutamate / Q000819:agonists*; D012160:Retina / Q000166:cytology
D000613:Aminobutyrates; D015220:Calcium Channels; D018690:Excitatory Amino Acid Agonists; D005456:Fluorescent Dyes; D018696:Neuroprotective Agents; D018094:Receptors, Metabotropic Glutamate; D003515:Cycloleucine; C036361:1-amino-1,3-dicarboxycyclopentane; D000255:Adenosine Triphosphate; D016318:Quisqualic Acid; C012729:2-amino-4-phosphonobutyric acid