title: A 1.2-megabase BAC/PAC contig spanning the 14q13 breakpoint of t(2; 14) in a mirror-image polydactyly patient.
abstract: We previously assigned a 14q13 breakpoint of t(2; 14) in a patient with mirror-image polydactyly to a segment between two loci, AFM200ZH4 and D14S306, within a genetic distance of 0.6 cM. In the present study, we constructed a 1.2-Mb high-resolution physical map with a contig composed of 16 bacterial artificial chromosomes (BACs) and 6 P1-derived artificial chromosomes (PACs) at a region around the breakpoint, extending from D14S75 to D14S728 loci. Thirty-four novel sequence-tagged sites (STSs) were also characterized at this region. Of nine ESTs that had been mapped between D14S75 and D14S288, T99065 was confirmed to be in two BAC clones, B102 and B319. This BAC/PAC contig with STSs is useful for further genomic sequencing, for construction of a transcription map, and for the isolation of the putative gene for mirror-image polydactyly.
authors.affiliation: Department of Human Genetics, Nagasaki University School of Medicine, Japan. f0932@cc.nagasaki-u.ac.jp
authors.forename: N
authors.initials: N
authors.lastname: Matsumoto
authors.forename: E
authors.initials: E
authors.lastname: Soeda
authors.forename: H
authors.initials: H
authors.lastname: Ohashi
authors.forename: M
authors.initials: M
authors.lastname: Fujimoto
authors.forename: R
authors.initials: R
authors.lastname: Kato
authors.forename: T
authors.initials: T
authors.lastname: Tsujita
authors.forename: H
authors.initials: H
authors.lastname: Tomita
authors.forename: S
authors.initials: S
authors.lastname: Kondo
authors.forename: Y
authors.initials: Y
authors.lastname: Fukushima
authors.forename: N
authors.initials: N
authors.lastname: Niikawa
mesh_terms: D002874:Chromosome Mapping; D002883:Chromosomes, Human, Pair 14; D002889:Chromosomes, Human, Pair 2; D003001:Cloning, Molecular; D006801:Humans; D008297:Male; D017689:Polydactyly / Q000235:genetics*; D016324:Sequence Tagged Sites; D014178:Translocation, Genetic