title: A cis-regulatory element within the 5' flanking region of arylsulfatase gene of sea urchin, Hemicentrotus pulcherrimus.
abstract: The 5' flanking region of the sea urchin Hemicentrotus pulcherrimus arylsulfatase (Ars) gene was scanned to define cis-regulatory elements required for proper expression congruent to that of the endogenous gene. The region between -100 bp and +38 bp from the transcription start site contains minimum information required for temporal initiation of transcription of the Ars gene. Progressive deletion analysis of Ars-luciferase reporter constructs containing the Ars sequence from -3484 bp to +38 bp suggests the existence of several cis-regulatory elements within this region. Results from luciferase assays of internal deletion mutants show strong enhancer activity detected within the sequence from -194 bp to -144 bp. By gel mobility shift assay, we have identified a nuclear factor that interacts sequence-specifically with this 50 bp region, and appears in a developmental stage-specific manner. Further deletion analysis determined that the enhancer activity lies within a 22 bp sequence between -186 bp and -164 bp.
authors.affiliation: Graduate Department of Gene Science, Faculty of Science, Hiroshima University, Japan.
authors.forename: J
authors.initials: J
authors.lastname: Morokuma
authors.forename: K
authors.initials: K
authors.lastname: Akasaka
authors.forename: K
authors.initials: K
authors.lastname: Mitsunaga-Nakatsubo
authors.forename: H
authors.initials: H
authors.lastname: Shimada
mesh_terms: D000818:Animals; D019677:Artificial Gene Fusion; D001192:Arylsulfatases / Q000235:genetics*; D001483:Base Sequence; D004247:DNA / Q000235:genetics; D017931:DNA Primers / Q000235:genetics; D004742:Enhancer Elements, Genetic; D005260:Female; D018507:Gene Expression Regulation, Developmental; D005809:Genes, Regulator; D017930:Genes, Reporter; D008156:Luciferases / Q000235:genetics; D009865:Oocytes / Q000201:enzymology; D012617:Sea Urchins / Q000201:enzymology* / Q000235:genetics* / Q000254:growth & development; D017384:Sequence Deletion
chemical_list: D017931:DNA Primers; D004247:DNA; D008156:Luciferases; D001192:Arylsulfatases