Replication of murine coronaviruses in mouse embryonic stem cell lines in vitro.
Replication of murine coronaviruses in eight mouse embryonic stem (ES) cell lines of several genetic backgrounds was examined. Both mouse hepatitis virus (MHV) type 2 and MHV, strain A59 replicated well with no or minimal cytopathic effect in all the ES cell lines tested. The results suggest the possibility that MHV-infected ES cells may disseminate MHV in mouse colonies due to embryo manipulation.
Kyuwa
S
S
Department of Animal Pathology, University of Tokyo, Japan.
D000818:Animals; D002460:Cell Line; D018562:Disease Transmission, Infectious; D004622:Embryo, Mammalian / Q000821:virology; D005818:Genetic Engineering; D066298:In Vitro Techniques; D051379:Mice; D006517:Murine hepatitis virus / Q000502:physiology*; D013234:Stem Cells / Q000821:virology; D014779:Virus Replication