Effect of cytochalasin D on systemic and local anaphylaxis in a murine model.
We investigated the effect of cytochalasin D on anaphylaxis. Cytochalasin D dose-dependently inhibited systemic anaphylaxis induced by compound 48/80 in rats. Especially, cytochalasin D inhibited compound 48/80-induced systemic anaphylaxis 100% with a dose of 1 microg g-1 body weight (BW). Cytochalasin D significantly inhibited serum histamine levels induced by compound 48/80. Cytochalasin D (10(-1) microg g-1 BW) also inhibited local anaphylaxis activated by anti-dinitrophenyl (DNP) IgE to 79.6+/-1.8%. Cytochalasin D dose-dependently inhibited histamine release from the rat peritoneal mast cells activated by compound 48/80 or anti-DNP IgE. The level of cAMP in rat peritoneal mast cells, when cytochalasin D was added, transiently and significantly increased about fourfold compared with that of basal cells. Our studies provide evidence that cytochalasin D will be beneficial in the treatment of anaphylaxis.
Shin
H Y
HY
College of Pharmacy, Wonkwang University, Iksan, South Korea.
Lee
E H
EH
Shin
T Y
TY
Kim
H M
HM
D000707:Anaphylaxis / Q000139:chemically induced / Q000517:prevention & control*; D000818:Animals; D018927:Anti-Asthmatic Agents / Q000627:therapeutic use; D004205:Cromolyn Sodium / Q000627:therapeutic use; D000242:Cyclic AMP / Q000378:metabolism*; D015638:Cytochalasin D / Q000627:therapeutic use*; D006636:Histamine Release / Q000187:drug effects*; D007073:Immunoglobulin E; D008407:Mast Cells / Q000187:drug effects* / Q000378:metabolism; D019384:Nucleic Acid Synthesis Inhibitors / Q000627:therapeutic use*; D010323:Passive Cutaneous Anaphylaxis / Q000187:drug effects; D051381:Rats; D017208:Rats, Wistar; D003189:p-Methoxy-N-methylphenethylamine
D018927:Anti-Asthmatic Agents; D019384:Nucleic Acid Synthesis Inhibitors; D015638:Cytochalasin D; D007073:Immunoglobulin E; D003189:p-Methoxy-N-methylphenethylamine; D000242:Cyclic AMP; D004205:Cromolyn Sodium