title: Alphaviruses as expression vectors.
abstract: Alphavirus vectors have been used for efficient high-level expression of a variety of topologically different proteins, allowing studies of protein transport, localization and functional activity in a broad range of host cells. Complex transmembrane proteins have been produced in large quantities through the establishment of scale-up technology. Alphavirus vectors have also shown promising potential in vaccine production and preliminary gene therapy applications.
authors.affiliation: F Hoffmann-La Roche, Research Laboratories, Roche Ltd, Basel, Switzerland. Kenneth.Lundstrom@roche.com
authors.forename: K
authors.initials: K
authors.lastname: Lundstrom
mesh_terms: D000524:Alphavirus / Q000235:genetics*; D000818:Animals; D001709:Biotechnology / Q000379:methods; D002460:Cell Line; D015316:Genetic Therapy / Q000379:methods; D005822:Genetic Vectors; D006801:Humans; D011994:Recombinant Proteins / Q000096:biosynthesis*; D014162:Transfection / Q000379:methods*; D014614:Vaccines, Synthetic / Q000096:biosynthesis
chemical_list: D011994:Recombinant Proteins; D014614:Vaccines, Synthetic