Comparison of Cu,Zn-superoxide dismutases of clawed frogs of the genus Xenopus.
Isoenzyme profiles and thermal stability of Cu,Zn-superoxide dismutase (Cu,Zn-SOD) of clawed frogs of genus Xenopus (X. laevis and X. borealis) were compared. Earlier the presence of an unusually thermolabile mutant Cu,Zn-SOD as well as normal enzyme was shown in X. laevis. Having confirmed the data, we further show that X. borealis contains only the thermolabile Cu,Zn-SOD. This suggested that a mutation in the Cu,Zn-SOD gene and genome duplication have occurred in the immediate predecessor of X. laevis. Later on the parent Cu,Zn-SOD gene was lost, leaving only the mutated form of Cu,Zn-SOD in X. borealis.
Peskin
A V
AV
Kol'tsov Institute of Developmental Biology, Russian Academy of Sciences, Moscow, Russia. peskin@ibrran.msk.su
Stolyarov
S D
SD
D000818:Animals; D004795:Enzyme Stability; D019143:Evolution, Molecular; D006801:Humans; D007527:Isoenzymes / Q000235:genetics / Q000378:metabolism*; D009154:Mutation; D010802:Phylogeny; D013045:Species Specificity; D013482:Superoxide Dismutase / Q000235:genetics / Q000378:metabolism*; D013696:Temperature; D014981:Xenopus / Q000235:genetics / Q000378:metabolism*; D014982:Xenopus laevis / Q000235:genetics / Q000378:metabolism*
D007527:Isoenzymes; D013482:Superoxide Dismutase