Purinergic modulation of rat urinary bladder detrusor smooth muscle.
1. Rat detrusor muscle was responsive to both ATP and adenosine; ATP elicited an excitatory response, whereas adenosine had an inhibitory effect. 2. ATP and adenosine had an inhibitory modulatory action on responses to acetylcholine, potassium depolarization and field stimulation. 3. Quinidine inhibited the ATP response and blocked the inhibitory effect of ATP on acetylcholine, potassium-depolarization and field-stimulation responses. The effect of adenosine remained unaltered in the presence of quinidine. 4. Caffeine and theophylline blocked the adenosine inhibition of responses to field stimulation. 5. It is concluded that excitatory P2-type purinoreceptors mediated by ATP and inhibitory P1-type purinoreceptors mediated by adenosine exist in rat urinary bladder detrusor smooth muscle and that both ATP and adenosine exhibit a modulatory action on detrusor muscle agonist-induced responses.
King
J A
JA
Division of Biological Sciences, Lancaster University, United Kingdom.
Huddart
H
H
Staff
W G
WG
D000241:Adenosine / Q000494:pharmacology; D000255:Adenosine Triphosphate / Q000494:pharmacology; D000818:Animals; D001285:Atropine / Q000494:pharmacology; D002110:Caffeine / Q000494:pharmacology; D004558:Electric Stimulation; D066298:In Vitro Techniques; D007741:Labetalol / Q000494:pharmacology; D008297:Male; D009130:Muscle, Smooth / Q000187:drug effects*; D058913:Purinergic Agonists; D058914:Purinergic Antagonists; D058915:Purinergic P1 Receptor Antagonists; D058919:Purinergic P2 Receptor Antagonists; D011802:Quinidine / Q000494:pharmacology; D051381:Rats; D017208:Rats, Wistar; D011983:Receptors, Purinergic / Q000187:drug effects / Q000502:physiology*; D001743:Urinary Bladder / Q000187:drug effects*
D058913:Purinergic Agonists; D058914:Purinergic Antagonists; D058915:Purinergic P1 Receptor Antagonists; D058919:Purinergic P2 Receptor Antagonists; D011983:Receptors, Purinergic; D002110:Caffeine; D001285:Atropine; D000255:Adenosine Triphosphate; D011802:Quinidine; D000241:Adenosine; D007741:Labetalol