title: Fc receptor-mediated phagocytosis, superoxide production and calcium signaling of beta 2 integrin-deficient bovine neutrophils.
abstract: Fc receptor for immunoglobulin G-mediated phagocytosis, superoxide production and intracellular calcium ([Ca2+]i) signaling of complement receptor type 3 (CR3)-deficient neutrophils from a heifer with leukocyte adhesion deficiency (BLAD) were compared to those of control heifers. The mean phagocytic activity of IgG-coated yeasts and aggregated bovine IgG (Agg-IgG)-induced superoxide production of CR3-deficient neutrophils were 10% and 77.9%, respectively, of those of control neutrophils. The [Ca2+]i signals in CR3-deficient neutrophils stimulated with Agg-IgG or concanavalin A were different with mean peak [Ca2+]i concentrations of 78% and 41.9%, respectively, of those of control neutrophils. These findings suggest that Fc receptor-mediated neutrophil functions are closely dependent on the presence of CR3 (CD11b/CD18) on the neutrophil cell surfaces.
authors.affiliation: Department of Animal Health, School of Veterinary Medicine, Rakuno Gakuen University, Ebetsu, Hokkaido, Japan.
authors.forename: H
authors.initials: H
authors.lastname: Nagahata
authors.forename: C
authors.initials: C
authors.lastname: Sawada
authors.forename: H
authors.initials: H
authors.lastname: Higuchi
authors.forename: H
authors.initials: H
authors.lastname: Teraoka
authors.forename: M
authors.initials: M
authors.lastname: Yamaguchi
mesh_terms: D000818:Animals; D018821:CD18 Antigens / Q000235:genetics / Q000502:physiology*; D002118:Calcium / Q000502:physiology*; D002417:Cattle; D002418:Cattle Diseases / Q000276:immunology / Q000473:pathology; D003208:Concanavalin A / Q000494:pharmacology; D003574:Cytochrome c Group / Q000378:metabolism; D005260:Female; D007074:Immunoglobulin G / Q000502:physiology*; D018370:Leukocyte-Adhesion Deficiency Syndrome / Q000276:immunology / Q000473:pathology / Q000662:veterinary; D009504:Neutrophils / Q000502:physiology*; D010587:Phagocytosis / Q000502:physiology*; D017452:Receptors, IgG / Q000502:physiology*; D015290:Second Messenger Systems / Q000502:physiology*; D013481:Superoxides / Q000378:metabolism*
chemical_list: D018821:CD18 Antigens; D003574:Cytochrome c Group; D007074:Immunoglobulin G; D017452:Receptors, IgG; D003208:Concanavalin A; D013481:Superoxides; D002118:Calcium