Extraction of DNA from human skeletal remains: practical applications in forensic sciences.
We have developed an efficient protocol for the extraction of DNA from bone samples. The protocol precludes decalcification and associates an efficient extraction of the DNA with removal of inhibitors by binding of DNA to silica particles. Two forensic cases are described in detail to demonstrate the usefulness of the method.
Prado
V F
VF
Departamento de Bioquímica e Imunologia, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Belo Horizonte, Brazil. VPRADO@ICB.UFMG.BR
Castro
A K
AK
Oliveira
C L
CL
Souza
K T
KT
Pena
S D
SD
D001842:Bone and Bones / Q000737:chemistry* / Q000473:pathology; D002648:Child; D004272:DNA, Mitochondrial / Q000032:analysis*; D005260:Female; D005554:Forensic Medicine; D006708:Homicide; D006801:Humans; D008297:Male; D010334:Paternity
D004272:DNA, Mitochondrial