title: Immunoreactivity of canine mammary neoplasms with monoclonal antibody CC49.
abstract: Monoclonal antibody (MAb) CC49 binds to human tumour-associated glycoprotein termed TAG-72. CC49 is a second-generation MAb with higher affinity to TAG-72 than the original MAb B72.3. CC49 was applied to 42 samples from different canine mammary tumours, belonging to seven different histopathological types. Immunoreactivity was detected by the use of an avidin-biotin complex immunoperoxidase method. Most sections from all types of mammary neoplasm reacted with this MAb. Normal tissue did not stain or stained only weakly. The results of this study suggest CC49 has selective immunoreactivity for a variety of canine mammary tumours, which seems superior to that reported with MAb 72.3. These results support the proposal for further study of diagnostic and therapeutic uses of CC49 in the management of canine mammary tumours.
authors.affiliation: Department of Clinical Sciences, Koret School of Veterinary Medicine, Hebrew University of Jerusalem, Israel.
authors.forename: R
authors.initials: R
authors.lastname: Shahar
authors.forename: A
authors.initials: A
authors.lastname: Harmelin
authors.forename: M H
authors.initials: MH
authors.lastname: Shamir
authors.forename: S
authors.initials: S
authors.lastname: Schneebaum
mesh_terms: D000230:Adenocarcinoma / Q000276:immunology / Q000473:pathology / Q000662:veterinary*; D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology* / Q000378:metabolism / Q000627:therapeutic use; D000912:Antibodies, Neoplasm / Q000276:immunology* / Q000378:metabolism / Q000627:therapeutic use; D000951:Antigens, Neoplasm / Q000032:analysis / Q000276:immunology*; D001706:Biopsy / Q000379:methods / Q000662:veterinary; D004283:Dog Diseases / Q000188:drug therapy / Q000276:immunology* / Q000378:metabolism; D004285:Dogs; D005260:Female; D006023:Glycoproteins / Q000032:analysis / Q000276:immunology*; D006801:Humans; D008321:Mammary Glands, Animal / Q000737:chemistry / Q000276:immunology / Q000473:pathology; D015674:Mammary Neoplasms, Animal / Q000276:immunology* / Q000473:pathology
chemical_list: D000911:Antibodies, Monoclonal; D000912:Antibodies, Neoplasm; D000951:Antigens, Neoplasm; C091202:B72.3 antibody; D006023:Glycoproteins; C047052:tumor-associated antigen 72