Soluble non-starch polysaccharides derived from complex food matrices do not increase average lipid droplet size during gastric lipid emulsification in rats.
The creation of a finely dispersed lipid emulsion is essential for efficient hydrolysis of dietary triglycerides. The effectiveness of emulsification within the stomach depends upon the shear force generated by gastric motility and the concentration of emulsifiers present in the gastric contents. Other dietary constituents can modify these factors, and previous studies have suggested that the presence of soluble non-starch polysaccharides (NSP) during digestion might increase the average size of intraluminal emulsion droplets. In the present study, we developed a new technique for the isolation and analysis of intraluminal lipid emulsions by optical diffraction analysis. The method was applied to rats fed powdered semipurified diets that were free of all NSP or supplemented with insoluble cellulose, guar gum, or NSP derived from apple, carrot or rolled oats. Cellulose had no significant effect on emulsion size, and there was no evidence that the average sizes of lipid droplets in the gastric fundus or antrum were higher than control values in rats fed diets supplemented with any source of soluble NSP. In the groups fed oats and cooked carrot NSP, the mean droplet diameters approached half the values for diets free of NSP or containing insoluble cellulose. The difference between rats fed NSP from cooked carrot and those fed cellulose was significant in the proximal stomach (P < 0.05), and that between rats fed raw oats and rats fed cellulose was significant in the distal stomach (P < 0.05). Soluble dietary fiber does not inhibit lipid or cholesterol absorption via any inhibition of lipid emulsification.
Fillery-Travis
A J
AJ
Institute of Food Research, Norwich Research Park, Colney, Norwich NR4 7UA, United Kingdom.
Gee
J M
JM
Waldron
K W
KW
Robins
M M
MM
Johnson
I T
IT
D000042:Absorption / Q000502:physiology; D000818:Animals; D018554:Avena / Q000737:chemistry / Q000378:metabolism; D002482:Cellulose / Q000737:chemistry / Q000378:metabolism; D018552:Daucus carota / Q000737:chemistry / Q000378:metabolism; D004032:Diet; D004040:Dietary Carbohydrates / Q000378:metabolism / Q000494:pharmacology; D004041:Dietary Fats / Q000378:metabolism / Q000494:pharmacology; D004044:Dietary Proteins / Q000378:metabolism / Q000494:pharmacology; D004655:Emulsions; D005079:Excipients / Q000032:analysis / Q000737:chemistry / Q000494:pharmacology*; D005504:Food Analysis; D005685:Galactans / Q000737:chemistry / Q000378:metabolism; D005753:Gastric Mucosa / Q000378:metabolism*; D006868:Hydrolysis; D050356:Lipid Metabolism; D008055:Lipids / Q000032:analysis / Q000737:chemistry; D008297:Male; D008351:Mannans / Q000737:chemistry / Q000378:metabolism; D055095:Optics and Photonics; D010316:Particle Size; D053149:Plant Gums; D011134:Polysaccharides / Q000032:analysis / Q000737:chemistry / Q000494:pharmacology*; D051381:Rats; D017208:Rats, Wistar; D019685:Rosales / Q000737:chemistry / Q000378:metabolism; D013213:Starch / Q000032:analysis / Q000378:metabolism; D013270:Stomach / Q000737:chemistry / Q000502:physiology; D013997:Time Factors
D004040:Dietary Carbohydrates; D004041:Dietary Fats; D004044:Dietary Proteins; D004655:Emulsions; D005079:Excipients; D005685:Galactans; D008055:Lipids; D008351:Mannans; D053149:Plant Gums; D011134:Polysaccharides; D002482:Cellulose; D013213:Starch; C007894:guar gum