title: Exogenous surfactant and partial liquid ventilation: physiologic and pathologic effects.
abstract: We compared the effects of surfactant and partial liquid ventilation (PLV), and the impact of administration order, on oxygenation, respiratory system compliance (Crs), hemodynamics, and lung pathology in an animal lung injury model. We studied four groups: surfactant alone (S; n = 8); partial liquid ventilation alone (PLV-only; n = 8); surfactant followed by partial liquid ventilation (S-PLV; n = 8); and partial liquid ventilation-followed by surfactant (PLV-S; n = 8). Following treatments, all animals had improved oxygenation index (OI) and Crs. Animals in PLV groups showed continued improvement over 2 h (% change OI: PLV-S -83% versus S -47%, p < 0.05; % change Crs: S-PLV 73% versus S 13%, p < 0.05). We also saw administration-order effects: surfactant before PLV improved Crs (0.92 ml/cm H2O after surfactant versus 1.13 ml/cm H2O after PLV, p < 0.02) without changing OI, whereas surfactant after PLV did not change Crs and OI increased (5.01 after PLV versus 8.92 after surfactant, p < 0.03). Hemodynamics were not different between groups. Pathologic analysis demonstrated decreased lung injury in dependent lobes of all PLV-treated animals, and in all lobes of S-PLV animals, when compared with the lobes of the S animals (p < 0.05). We conclude that surfactant therapy in combination with PLV improved oxygenation, respiratory system mechanics, and lung pathology to a greater degree than surfactant therapy alone. Administration order affected initial physiologic response and ultimate pathology: surfactant given before PLV produced the greatest improvements in pathologic outcomes.
authors.affiliation: Infant Pulmonary Research Center, Children's Health Care-St. Paul, Minnesota 55102, USA.
authors.forename: J D
authors.initials: JD
authors.lastname: Mrozek
authors.forename: K M
authors.initials: KM
authors.lastname: Smith
authors.forename: D R
authors.initials: DR
authors.lastname: Bing
authors.forename: P A
authors.initials: PA
authors.lastname: Meyers
authors.forename: S C
authors.initials: SC
authors.lastname: Simonton
authors.forename: J E
authors.initials: JE
authors.lastname: Connett
authors.forename: M C
authors.initials: MC
authors.lastname: Mammel
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D001784:Blood Gas Analysis; D003187:Compliance / Q000187:drug effects; D004195:Disease Models, Animal; D005466:Fluorocarbons / Q000494:pharmacology*; D006439:Hemodynamics / Q000187:drug effects; D008168:Lung / Q000187:drug effects* / Q000503:physiopathology; D008171:Lung Diseases / Q000188:drug therapy / Q000473:pathology / Q000503:physiopathology*; D055370:Lung Injury; D011659:Pulmonary Gas Exchange / Q000187:drug effects; D011663:Pulmonary Surfactants / Q000494:pharmacology*; D011897:Random Allocation; D015656:Respiratory Mechanics / Q000187:drug effects*; D013552:Swine
chemical_list: D005466:Fluorocarbons; D011663:Pulmonary Surfactants