A*02 in southern Thai Muslims and central Thais.
The HLA-A*02 subtyping in Thais was conducted and included in the 12th International Histocompatibility Workshop (12WS). A total of 81 randomized individuals previously serologically or DNA typed as A2 were studied for A2 subtypings. The subjects consisted of 32 Southern Thai-Muslims (STM) and 49 Central Thais (CT). The 12WS HLA-A*02 subtyping DNA typing kit was employed. The most common A*02 subtypes in STM were A*0203,*0201 and *0207 while they were A*0203, *0207 and *0201 in CT. A*0202, *0204, *0208, *0209, *0212, *0213, *0214, *0215, *0216 and *0217 were not found in both STM and CT. The 12WS data indicated that A*0201 was also the most frequent allele of A*2 among North-East Asians. A2 subtype study in 32 STM revealed that 2 in 8 of A*0201 showed the absence of bands at 813 bp and 705 bp with primer mix number 03A and 517A and weak reaction band with primer mix number 33A. In addition, 3 subjects with A*0201 variations have one nucleotide difference in exon 2 by sequence base typing (by MGJ. Tilanus) which will be reported separately.


CONCLUSIONS
More variations of A*02 were observed among STM compared to CT. The variations of reactions with the set of primer mix should be carefully observed and subjected to further analysis.
Chiewsilp
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P
Department of Pathology, Faculty of Medicine, Ramathibodi Hospital, Mahidol University, Bangkok, Thailand.
Mongkolsuk
T
T
Sujirachato
K
K
D000483:Alleles; D016009:Chi-Square Distribution; D002681:China / Q000208:ethnology; D005006:Ethnicity / Q000235:genetics*; D005787:Gene Frequency; D015234:HLA-A Antigens / Q000145:classification / Q000235:genetics*; D006239:Haplotypes; D006801:Humans; D008296:Malaysia / Q000208:ethnology; D016133:Polymerase Chain Reaction / Q000379:methods; D013785:Thailand
D015234:HLA-A Antigens