Genotypic evaluation of Salmonella enteritidis isolates of known phage types by arbitrarily primed polymerase chain reaction.
Salmonella enteritidis isolates of known phage types 8, 13a, and 14b were inoculated separately into a group of 14-wk-old specific-pathogen-free chickens. S. enteritidis isolates from pre- and postinoculum were analyzed with the use of arbitrarily primed polymerase chain reaction to determine any genotypic changes after reisolation from the chickens. The preinoculum isolates and the isolates of S. enteritidis recovered at 5, 10, 20, and 35 days postinoculation from the inoculated chickens with various phage types were similar. Therefore, no changes of S. enteritidis organisms of similar phage types occurred at the genotypic level. However, SE-3-B7001 (phage type 8) was changed to phage type 14b after inoculation into the chickens.
Fadl
A A
AA
Department of Pathobiology, College of Agriculture and Natural Resources, University of Connecticut, Storrs 06269, USA.
Khan
M I
MI
D000818:Animals; D001434:Bacteriophage Typing; D002645:Chickens; D004269:DNA, Bacterial / Q000302:isolation & purification; D005838:Genotype; D016133:Polymerase Chain Reaction / Q000379:methods; D012481:Salmonella Infections, Animal / Q000382:microbiology; D012483:Salmonella Phages / Q000145:classification / Q000302:isolation & purification*; D012477:Salmonella enteritidis / Q000145:classification / Q000235:genetics* / Q000821:virology; D013047:Specific Pathogen-Free Organisms
D004269:DNA, Bacterial