title: Genetic mapping of five human chromosome 4 orthologues to bovine chromosomes 6 and 17.
abstract: Five loci that map to human chromosome 4 (HSA4) were selected to expand the bovine comparative linkage map. Loci included b-casein (CSN2), basic fibroblast growth factor (FGF2), immunoglobulin J chain (IGJ), interleukin 2 (IL2) and microsomal triglyceride transfer protein (MTTP). Polymorphisms for each locus were identified by either polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) or single-strand conformational polymorphism (SSCP) analysis. The bovine genes for CSN2, IGJ and MTTP were mapped by linkage analysis to chromosome 6; FGF2 and IL2 mapped to chromosome 17. These data refine a position of chromosomal evolution to a small region between FGF2 and the previously mapped complement I factor (IF).
authors.affiliation: Department of Animal Sciences, University of Illinois at Urbana-Champaign 61801, USA.
authors.forename: S R
authors.initials: SR
authors.lastname: Fisher
authors.forename: J E
authors.initials: JE
authors.lastname: Beever
authors.forename: H A
authors.initials: HA
authors.lastname: Lewin
mesh_terms: D000818:Animals; D001483:Base Sequence; D002352:Carrier Proteins / Q000235:genetics; D002364:Caseins / Q000235:genetics; D002417:Cattle / Q000235:genetics*; D002874:Chromosome Mapping / Q000662:veterinary*; D002894:Chromosomes, Human, Pair 4 / Q000235:genetics*; D017931:DNA Primers / Q000235:genetics; D016222:Fibroblast Growth Factor 2 / Q000235:genetics; D008040:Genetic Linkage; D006801:Humans; D007144:Immunoglobulin J-Chains / Q000235:genetics; D007376:Interleukin-2 / Q000235:genetics; D051379:Mice; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length; D018807:Polymorphism, Single-Stranded Conformational; D013045:Species Specificity
chemical_list: D002352:Carrier Proteins; D002364:Caseins; D017931:DNA Primers; D007144:Immunoglobulin J-Chains; D007376:Interleukin-2; C106092:microsomal triglyceride transfer protein; D016222:Fibroblast Growth Factor 2