title: Assay of 1,25-dihydroxyvitamin D3 from serum samples: use of receptor-binding or enzyme-coupled reporter analysis.
authors.affiliation: Department of Biochemistry, University of Wisconsin-Madison 53706, USA.
authors.forename: M J
authors.initials: MJ
authors.lastname: Beckman
authors.forename: H F
authors.initials: HF
authors.lastname: DeLuca
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal; D002117:Calcitriol / Q000097:blood* / Q000302:isolation & purification / Q000494:pharmacology; D002467:Cell Nucleus / Q000378:metabolism; D003577:Cytochrome P-450 Enzyme System; D004790:Enzyme Induction; D017930:Genes, Reporter; D006801:Humans; D007422:Intestines; D008156:Luciferases / Q000096:biosynthesis; D012516:Osteosarcoma; D011401:Promoter Regions, Genetic; D011869:Radioligand Assay; D051381:Rats; D018167:Receptors, Calcitriol / Q000378:metabolism*; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis; D012680:Sensitivity and Specificity; D013250:Steroid Hydroxylases / Q000096:biosynthesis; D013552:Swine; D014162:Transfection / Q000379:methods; D014316:Tritium; D014407:Tumor Cells, Cultured; D065668:Vitamin D3 24-Hydroxylase
chemical_list: D000911:Antibodies, Monoclonal; D018167:Receptors, Calcitriol; D011993:Recombinant Fusion Proteins; D014316:Tritium; D003577:Cytochrome P-450 Enzyme System; D008156:Luciferases; D013250:Steroid Hydroxylases; D065668:Vitamin D3 24-Hydroxylase; D002117:Calcitriol