Dermatophilus congolensis: strain differences in expression of phospholipase activities.
Interactions between Dermatophilus congolensis strains and with other bacteria of known haemolytic activities were used to elucidate the complex nature of haemolytic activities present in various D. congolensis strains. This was further analysed by measuring their specific phospholipase activities against defined substrates by thin layer chromatography. D. congolensis strains demonstrated haemolytic interactions (synergistic or antagonistic) with other D. congolensis strains and also other species of bacteria. Most isolates expressed lyso-phospholipase-D activity, while various strains also expressed sphingomyelinase-D activity, phospholipase-A versus phosphatidylcholines and/or cephalins, phospholipase-D versus phosphatidylcholines or all these activities, under the culture conditions used.
Masters
A M
AM
Animal Health Laboratories, Agriculture W.A, South Perth, Western Australia. trevore@aidpo.agric.wa.gov.au
Ellis
T M
TM
Grein
S B
SB
D000192:Actinomycetales / Q000145:classification* / Q000201:enzymology / Q000235:genetics; D000193:Actinomycetales Infections / Q000382:microbiology / Q000662:veterinary*; D000818:Animals; D002417:Cattle; D002418:Cattle Diseases; D006461:Hemolysis; D006734:Horse Diseases; D006736:Horses; D010739:Phospholipase D / Q000096:biosynthesis; D010740:Phospholipases / Q000096:biosynthesis*; D010741:Phospholipases A / Q000096:biosynthesis; D010727:Phosphoric Diester Hydrolases / Q000096:biosynthesis; D012756:Sheep; D012757:Sheep Diseases; D017192:Skin Diseases, Bacterial / Q000382:microbiology / Q000662:veterinary*; D000099189:Lysophospholipase D
D010739:Phospholipase D; D010740:Phospholipases; D010741:Phospholipases A; D010727:Phosphoric Diester Hydrolases; D000099189:Lysophospholipase D; C055160:sphingomyelin phosphodiesterase D