Detection of trichloroethylene-protein adducts in rat liver and plasma.
Trichloroethylene is an industrial chemical with widespread occupational exposure and is a major environmental contaminant. In a Western blot using antiserum that recognizes trichloroethylene covalently bound to protein, a single 50 kDa microsomal adduct was detected in the livers of trichloroethylene-treated Sprague-Dawley rats. To determine if trichloroethylene-protein adducts could be detected in blood, plasma proteins were immunoaffinity purified using an antidichloroacetyl column. A single 50 kDa protein was detected in the affinity-purified fraction in a Western blot using dichloroacetyl antiserum. This protein was also immunochemically reactive with anti-cytochrome P450 2E1 antibodies. The 50 kDa trichloroethylene-protein adduct may be formed in the liver and released into the blood following exposure to trichloroethylene. The significance of adduct formation with respect to trichloroethylene toxicity remains to be established; however, the data suggest that this approach may be useful in the investigation of trichloroethylene-protein adducts and adverse effects following exposure.
Halmes
N C
NC
Division of Toxicology, University of Arkansas for Medical Sciences, Little Rock 72205-7199, USA.
Perkins
E J
EJ
McMillan
D C
DC
Pumford
N R
NR
D000818:Animals; D015551:Autoimmunity / Q000187:drug effects; D015153:Blotting, Western; D019392:Cytochrome P-450 CYP2E1 / Q000378:metabolism; D004785:Environmental Pollutants / Q000378:metabolism*; D008099:Liver / Q000378:metabolism*; D008297:Male; D011485:Protein Binding; D051381:Rats; D017207:Rats, Sprague-Dawley; D014241:Trichloroethylene / Q000378:metabolism* / Q000633:toxicity
D004785:Environmental Pollutants; D014241:Trichloroethylene; D019392:Cytochrome P-450 CYP2E1