Molecular approaches to identifying ligand binding and signaling domains of C-C chemokine receptors.
The construction of chimeric receptors provides a useful starting point for the identification of ligand-binding and G-protein-coupling sites on chemokine receptors. Correlation of the binding and signaling properties of a set of complementary receptor chimeras is a powerful approach for probing structure-function relationships. Further molecular resolution can subsequently be achieved by site-directed mutagenesis and/or alanine scanning.
Monteclaro
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Gladstone Institute of Cardiovascular Disease, University of California, San Francisco 94141, USA.
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Charo
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D000595:Amino Acid Sequence; D001665:Binding Sites; D001667:Binding, Competitive; D002460:Cell Line; D002462:Cell Membrane / Q000378:metabolism; D019204:GTP-Binding Proteins / Q000378:metabolism; D006801:Humans; D007295:Inositol Phosphates / Q000378:metabolism; D008024:Ligands; D008958:Models, Molecular; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D011485:Protein Binding; D054389:Receptors, CCR1; D054390:Receptors, CCR2; D019707:Receptors, Chemokine / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*; D011993:Recombinant Fusion Proteins / Q000737:chemistry / Q000378:metabolism; D016415:Sequence Alignment; D015398:Signal Transduction / Q000502:physiology; D013379:Substrate Specificity; D014162:Transfection
C515300:CCR1 protein, human; C515334:CCR2 protein, human; D007295:Inositol Phosphates; D008024:Ligands; D054389:Receptors, CCR1; D054390:Receptors, CCR2; D019707:Receptors, Chemokine; D011993:Recombinant Fusion Proteins; D019204:GTP-Binding Proteins