title: Oxygen radicals diminish dopamine transporter function in rat striatum.
abstract: Incubation of striatal synaptosomes with the oxygen radical generating enzyme, xanthine oxidase, decreased [3H]dopamine uptake: an effect attributable to a decreased Vmax. Concurrent incubation with the superoxide radical scavenger, superoxide dismutase, abolished the xanthine oxidase-induced decrease. These results indicate that, like methamphetamine administration in vivo, reactive oxygen species diminish dopamine transporter function in vitro. The significance of these findings to mechanisms responsible for effects of methamphetamine is discussed.
authors.affiliation: Department of Pharmacology and Toxicology, University of Utah, Salt Lake City 84112, USA.
authors.forename: A E
authors.initials: AE
authors.lastname: Fleckenstein
authors.forename: R R
authors.initials: RR
authors.lastname: Metzger
authors.forename: M L
authors.initials: ML
authors.lastname: Beyeler
authors.forename: J W
authors.initials: JW
authors.lastname: Gibb
authors.forename: G R
authors.initials: GR
authors.lastname: Hanson
mesh_terms: D000818:Animals; D003342:Corpus Striatum / Q000378:metabolism*; D004298:Dopamine / Q000378:metabolism*; D018765:Dopamine Uptake Inhibitors / Q000494:pharmacology*; D008297:Male; D008694:Methamphetamine / Q000494:pharmacology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D017382:Reactive Oxygen Species / Q000378:metabolism*; D013482:Superoxide Dismutase / Q000378:metabolism; D014969:Xanthine Oxidase / Q000378:metabolism
chemical_list: D018765:Dopamine Uptake Inhibitors; D017382:Reactive Oxygen Species; D008694:Methamphetamine; D013482:Superoxide Dismutase; D014969:Xanthine Oxidase; D004298:Dopamine