Study of the metabolism of choline and phosphatidylcholine in tumors in vivo using phosphonium-choline.
The results of an initial study on the feasibility of using the phosphonium analog of choline to follow the metabolism of phosphatidylcholine in tumors in vivo using 31P NMR are reported. C3H/He mice bearing a mammary carcinoma tumor on the foot pad were fed a choline-free diet supplemented with the phosphonium analog of choline. Metabolites of this compound, including the phosphonium analogs of phosphatidylcholine, phosphocholine, glycerophosphocholine, and betaine were observed noninvasively in vivo in tumors by 31P NMR after 2-3 weeks of feeding. Clearance of these phosphonium-labeled metabolites from tumors was measured after a change to a choline-containing diet. Significant decreases were seen in the levels of the analogs of betaine (P < 0.003) and phosphatidylcholine (P < 0.004) by Day 4. A significant increase in the level of authentic phosphocholine (P < 0.003) occurred over the same time period.
Street
J C
JC
Department of Medical Physics, Memorial Sloan-Kettering Cancer Center, New York, NY 10021, USA.
Szwergold
B S
BS
Matei
C
C
Kappler
F
F
Mahmood
U
U
Brown
T R
TR
Koutcher
J A
JA
D000230:Adenocarcinoma / Q000378:metabolism*; D000818:Animals; D002794:Choline / Q000031:analogs & derivatives* / Q000378:metabolism*; D005260:Female; D009682:Magnetic Resonance Spectroscopy; D008325:Mammary Neoplasms, Experimental / Q000378:metabolism*; D051379:Mice; D008809:Mice, Inbred C3H; D009943:Organophosphorus Compounds; D010713:Phosphatidylcholines / Q000378:metabolism*
D009943:Organophosphorus Compounds; D010713:Phosphatidylcholines; C093663:phosphonium choline; D002794:Choline