Trophic effects of melanotropin-potentiating factor (MPF) on cultures of cells of the central nervous system.
MPF is a tetrapeptide (structure Lys-Lys-Gly-Glu) that elicits a variety of neurotrophic effects in vivo consistent with a role in neuronal regeneration. In support of this role, we now show that MPF stimulates the proliferation of cultured astrocytes and neurite outgrowth from cultures of neocortical cholinergic and mesenchephalic dopaminergic neurons. The dose-response relationships are biphasic ("bell shaped"), maximal responses being obtained with 10(-6) M concentrations of MPF. MPF and nerve growth factor seem to act on different receptors, because their effects on cholinergic neurons are synergistic.
Owen
D B
DB
Department of Evolutionary & Environmental Biology, University of Liverpool, UK.
Morley
J S
JS
Ensor
D M
DM
Allen
Y S
YS
Miles
J B
JB
D000324:Adrenocorticotropic Hormone / Q000378:metabolism; D000818:Animals; D001253:Astrocytes / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D002455:Cell Division / Q000187:drug effects; D002478:Cells, Cultured; D002490:Central Nervous System / Q000166:cytology* / Q000187:drug effects* / Q000378:metabolism; D002540:Cerebral Cortex / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D003561:Cytarabine / Q000494:pharmacology; D004298:Dopamine / Q000378:metabolism; D004305:Dose-Response Relationship, Drug; D004723:Endorphins / Q000008:administration & dosage / Q000494:pharmacology*; D006801:Humans; D008636:Mesencephalon / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D008959:Models, Neurological; D009414:Nerve Growth Factors / Q000494:pharmacology; D009474:Neurons / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D010446:Peptide Fragments / Q000008:administration & dosage / Q000494:pharmacology*; D051381:Rats
D004723:Endorphins; D009414:Nerve Growth Factors; D010446:Peptide Fragments; D003561:Cytarabine; C050079:lysyl-lysyl-glycyl-glutamic acid; D000324:Adrenocorticotropic Hormone; D004298:Dopamine