Evidence for direct cellular protective effect of PL-10 substances (synthesized parts of body protection compound, BPC) and their specificity to gastric mucosal cells.
The direct gastric mucosal cellular effect of four PL-10 substances (a synthesized part of human body protection compound, BPC containing 14 or 15 amino acids) was studied on freshly isolated rat gastric mucosal cells and on a mouse myeloma cell line (Sp2/0-Ag14) in an ethanol-induced cell injury model. The examined substances were not toxic for the cells. Two of them proved to be significantly protective against the direct cellular damaging effect of ethanol (PL 10.1.15AK-3 in 5 microg/ml dose and PL 10.1.AK14-2 dose-dependently, ED50=50 ng/ml) on gastric mucosal cells. This cytoprotective effect was failured on mouse myeloma cells. Based on these results a part of the in vivo protection induced by BPC seems to be a direct cellular protective effect to gastric mucosal cells.
Bódis
B
B
First Department of Medicine, University Medical School of Pécs, Hungary.
Karádi
O
O
Németh
P
P
Dohoczky
C
C
Kolega
M
M
Mózsik
G
G
D000818:Animals; D002470:Cell Survival; D019610:Cytoprotection; D000431:Ethanol / Q000633:toxicity; D005753:Gastric Mucosa / Q000187:drug effects*; D006801:Humans; D051379:Mice; D009101:Multiple Myeloma; D010446:Peptide Fragments / Q000494:pharmacology*; D011506:Proteins / Q000494:pharmacology*; D051381:Rats; D013385:Succinate Dehydrogenase / Q000378:metabolism; D014343:Trypan Blue; D014407:Tumor Cells, Cultured
D010446:Peptide Fragments; D011506:Proteins; D000431:Ethanol; C083833:BPC 157; D013385:Succinate Dehydrogenase; D014343:Trypan Blue