Detailed physical analysis of a 1.5-megabase YAC contig containing the MXI1 and ADRA2A genes.
The distal long arm of chromosome 10 harbors genes of biomedical interest such as MXI1, a putative tumor suppressor gene, and those encoding the adrenergic receptors alpha2A (ADRA2A) and beta1 (ADRB1). As part of a physical and genetic study of this genomic region, we constructed a 1.5-Mb YAC contig mapping to 10q25 that contains MXI1 and ADRA2A as well as a number of STSs. Rare cutting restriction site analysis of overlapping YACs allowed fine mapping of these genes and markers along the contig and revealed the presence of four CpG islands. MXI1 and ADRA2A appear to be about 600 kb apart, whereas ADRB1 is separated from ADRA2A by a distance larger than previously reported.
Manca
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A
Istituto di Genetica Molecolare del CNR, Alghero, Italy.
Volpi
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Laficara
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Muresu
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Gray
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IC
Spurr
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NK
Nobile
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C
D051792:Basic Helix-Loop-Helix Proteins; D002874:Chromosome Mapping; D018244:Chromosomes, Artificial, Yeast / Q000235:genetics; D002879:Chromosomes, Human, Pair 10 / Q000235:genetics; D018899:CpG Islands; D004268:DNA-Binding Proteins / Q000235:genetics*; D016147:Genes, Tumor Suppressor; D005819:Genetic Markers; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D018341:Receptors, Adrenergic, alpha-2 / Q000235:genetics*; D015183:Restriction Mapping; D016324:Sequence Tagged Sites; D014157:Transcription Factors / Q000235:genetics*; D025521:Tumor Suppressor Proteins
D051792:Basic Helix-Loop-Helix Proteins; D004268:DNA-Binding Proteins; D005819:Genetic Markers; D018341:Receptors, Adrenergic, alpha-2; D014157:Transcription Factors; D025521:Tumor Suppressor Proteins; C079440:MXI1 protein, human