title: Assay of 25-hydroxyvitamin D 1 alpha-hydroxylase and 24-hydroxylase.
authors.affiliation: Department of Biochemistry, University of Wisconsin-Madison 53706, USA.
authors.forename: M J
authors.initials: MJ
authors.lastname: Beckman
authors.forename: H F
authors.initials: HF
authors.lastname: DeLuca
mesh_terms: D015090:25-Hydroxyvitamin D3 1-alpha-Hydroxylase / Q000032:analysis* / Q000302:isolation & purification / Q000378:metabolism; D000818:Animals; D002112:Calcifediol / Q000138:chemical synthesis / Q000378:metabolism; D002460:Cell Line; D002645:Chickens; D002846:Chromatography, Affinity / Q000379:methods; D002852:Chromatography, Ion Exchange / Q000379:methods; D003577:Cytochrome P-450 Enzyme System / Q000032:analysis* / Q000302:isolation & purification / Q000378:metabolism; D007668:Kidney / Q000201:enzymology*; D007684:Kidney Tubules / Q000201:enzymology; D007700:Kinetics; D008928:Mitochondria / Q000201:enzymology*; D011865:Radioisotope Dilution Technique; D011994:Recombinant Proteins / Q000032:analysis / Q000302:isolation & purification / Q000378:metabolism; D018411:Spodoptera; D013250:Steroid Hydroxylases / Q000032:analysis* / Q000302:isolation & purification / Q000378:metabolism; D014162:Transfection / Q000379:methods; D014316:Tritium; D065668:Vitamin D3 24-Hydroxylase
chemical_list: D011994:Recombinant Proteins; D014316:Tritium; D003577:Cytochrome P-450 Enzyme System; D013250:Steroid Hydroxylases; D065668:Vitamin D3 24-Hydroxylase; D015090:25-Hydroxyvitamin D3 1-alpha-Hydroxylase; D002112:Calcifediol