title: Differential display analysis of RNA accumulation in arbuscular mycorrhiza of pea and isolation of a novel symbiosis-regulated plant gene.
abstract: Differential RNA display was used to analyze gene expression during the early steps of mycorrhiza development on Pisum sativum following inoculation with Glomus mosseae. Seven out of 118 differentially displayed cDNA fragments were subcloned and sequenced. One fragment corresponded to part of the fungal 25S ribosomal RNA gene and a second one showed similarity to a human Alu element. The others were derived from plant genes of unknown function. One of the fragments was used for the isolation of a full-length cDNA clone. It corresponded to a single-copy gene (psam1) which is induced during early symbiotic interactions, and codes for a putative transmembrane protein. Northern and RNA dot blot analyses revealed enhanced accumulation of psam1 RNA after inoculation with G. mosseae of wild-type pea and an isogenic mutant deficient for nodule development (Nod-, Myc+).
authors.affiliation: Laboratoire de Phytoparasitologie, INRA/CNRS, CMSE-INRA, Dijon, France.
authors.forename: F
authors.initials: F
authors.lastname: Martin-Laurent
authors.forename: D
authors.initials: D
authors.lastname: van Tuinen
authors.forename: E
authors.initials: E
authors.lastname: Dumas-Gaudot
authors.forename: V
authors.initials: V
authors.lastname: Gianinazzi-Pearson
authors.forename: S
authors.initials: S
authors.lastname: Gianinazzi
authors.forename: P
authors.initials: P
authors.lastname: Franken
mesh_terms: D001483:Base Sequence; D015152:Blotting, Northern; D015139:Blotting, Southern; D003001:Cloning, Molecular; D018076:DNA, Complementary; D004591:Electrophoresis, Polyacrylamide Gel; D005658:Fungi / Q000235:genetics / Q000502:physiology*; D018506:Gene Expression Regulation, Plant; D017343:Genes, Plant; D008565:Membrane Proteins / Q000737:chemistry / Q000235:genetics*; D008969:Molecular Sequence Data; D018532:Pisum sativum / Q000235:genetics* / Q000378:metabolism / Q000382:microbiology*; D010940:Plant Proteins / Q000737:chemistry / Q000235:genetics*; D016133:Polymerase Chain Reaction; D012331:RNA, Fungal / Q000235:genetics; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D018749:RNA, Plant / Q000235:genetics / Q000378:metabolism; D012335:RNA, Ribosomal / Q000235:genetics; D017422:Sequence Analysis, DNA; D012689:Sequence Homology, Nucleic Acid; D013559:Symbiosis / Q000235:genetics*
chemical_list: D018076:DNA, Complementary; D008565:Membrane Proteins; C108802:PSAM1 protein, Pisum sativum; D010940:Plant Proteins; D012331:RNA, Fungal; D012333:RNA, Messenger; D018749:RNA, Plant; D012335:RNA, Ribosomal; C065039:RNA, ribosomal, 25S