title: Crystal structure of nitric oxide reductase from denitrifying fungus Fusarium oxysporum.
abstract: Structures of nitric oxide reductase (NOR) in the ferric resting and the ferrous CO states have been solved at 2.0 A resolution. These structures provide significant new insights into how NO is reduced in biological systems. The haem distal pocket is open to solvent, implicating this region as a possible NADH binding site. In combination with mutagenesis results, a hydrogen-bonding network from the water molecule adjacent to the iron ligand to the protein surface of the distal pocket through the hydroxyl group of Ser 286 and the carboxyl group of Asp 393 can be assigned to a pathway for proton delivery during the NO reduction reaction.
authors.affiliation: Institute of Physical and Chemical Research (RIKEN), Saitama, Japan.
authors.forename: S Y
authors.initials: SY
authors.lastname: Park
authors.forename: H
authors.initials: H
authors.lastname: Shimizu
authors.forename: S
authors.initials: S
authors.lastname: Adachi
authors.forename: A
authors.initials: A
authors.lastname: Nakagawa
authors.forename: I
authors.initials: I
authors.lastname: Tanaka
authors.forename: K
authors.initials: K
authors.lastname: Nakahara
authors.forename: H
authors.initials: H
authors.lastname: Shoun
authors.forename: E
authors.initials: E
authors.lastname: Obayashi
authors.forename: H
authors.initials: H
authors.lastname: Nakamura
authors.forename: T
authors.initials: T
authors.lastname: Iizuka
authors.forename: Y
authors.initials: Y
authors.lastname: Shiro
mesh_terms: D000595:Amino Acid Sequence; D001665:Binding Sites; D002248:Carbon Monoxide; D018360:Crystallography, X-Ray; D005670:Fusarium / Q000201:enzymology*; D006418:Heme; D007501:Iron; D008961:Models, Structural; D008969:Molecular Sequence Data; D009243:NAD / Q000378:metabolism; D010088:Oxidoreductases / Q000737:chemistry* / Q000378:metabolism; D011487:Protein Conformation; D017433:Protein Structure, Secondary; D011994:Recombinant Proteins / Q000737:chemistry / Q000378:metabolism; D012997:Solvents
chemical_list: D011994:Recombinant Proteins; D012997:Solvents; D009243:NAD; D006418:Heme; D002248:Carbon Monoxide; D007501:Iron; D010088:Oxidoreductases; C034747:nitric-oxide reductase