title: Aging- and ovariectomy-related skeletal changes in spontaneously hypertensive rats.
abstract: The skeletal impact of estrogen deficiency on subjects with hypertension has not been studied previously. In this study, we examined the skeletal characteristics of female spontaneously hypertensive rats (SHR) and their normotensive genetic control Wistar-Kyoto rat (WKY). We aimed to reveal: 1) the skeletal characters of female SHR, and 2) the response of SHR to ovariectomy (ovx) when compared to WKY and other strains.
  
  
  
  Undecalcified double-fluorescent labeled cancellous (proximal tibial metaphysis, PTM) and cortical (tibial shaft, TX) bones from 23-weeks-old, and from rats 2 and 8 weeks post-ovx were studied.
  
  
  
  The SHR showed lower body weight, higher heart rate, and higher blood pressure than the WKY. Female SHR possessed more percent cancellous bone, less net cortical bone, smaller tissue area, and thinner cortex than WKY. Furthermore, SHR exhibited an age-related cancellous (-18%) and cortical (-7%) bone loss associated with a decrease in the longitudinal growth rate and bone balance and a decrease in periosteal bone formation in cortical bone. In contrast, the WKY maintained most of these parameters unchanged at their 23-week-old levels. Ovariectomy induced earlier and greater cancellous bone loss in the SHR than in the WKY, with greater increases in bone turnover rate, eroded surface, activation frequency, and a decrease in the ratio of labeled to eroded perimeter in PTM at 2 weeks postsurgery. However, the two groups exhibited no differences in bone loss at 8 weeks after ovx in PTM and TX.
  
  
  
  Spontaneously hypertensive rats were highly sensitive to estrogen deficiency. This might have clinical relevance to those postmenopausal women who suffer from hypertension, in that they may be more susceptible to osteopenia. If so, preventive measures should be initiated sooner than otherwise.
authors.affiliation: Radiobiology Division, University of Utah School of Medicine, Salt Lake City 84112, USA.
authors.forename: H
authors.initials: H
authors.lastname: Liang
authors.forename: Y
authors.initials: Y
authors.lastname: Ma
authors.forename: S
authors.initials: S
authors.lastname: Pun
authors.forename: M
authors.initials: M
authors.lastname: Stimpel
authors.forename: W S
authors.initials: WS
authors.lastname: Jee
mesh_terms: D000375:Aging / Q000502:physiology*; D000818:Animals; D001835:Body Weight / Q000502:physiology; D001846:Bone Development / Q000502:physiology; D001842:Bone and Bones / Q000473:pathology / Q000503:physiopathology*; D004967:Estrogens / Q000172:deficiency; D005260:Female; D006439:Hemodynamics / Q000502:physiology; D006973:Hypertension / Q000473:pathology / Q000503:physiopathology*; D007091:Image Processing, Computer-Assisted; D010024:Osteoporosis / Q000473:pathology / Q000503:physiopathology; D010052:Ovariectomy; D051381:Rats; D011918:Rats, Inbred SHR; D011921:Rats, Inbred WKY; D013977:Tibia / Q000473:pathology
chemical_list: D004967:Estrogens