title: Osteoblast responses to BMP-2-treated titanium in vitro.
abstract: In this study, the phenotypic expression of osteoblast progenitor cells, 2T9, on characterized titanium surfaces was examined in the presence of a bone morphogenetic protein-2 (BMP-2). X-ray photoelectron spectroscopy spectra indicated the presence of titanium dioxide on titanium surfaces, along with surface contaminants such as carbon and nitrogen. In the in vitro study, the activity of BMP-2-treated osteoblast cells on titanium surfaces was marked by significantly higher alkaline phosphatase-specific activity and 1,25 (OH2) vitamin D3-stimulated osteocalcin production when compared to the untreated cells on titanium surfaces.
authors.affiliation: Department of Restorative Dentistry, University of Texas Health Science Center at San Antonio 78284, USA.
authors.forename: J L
authors.initials: JL
authors.lastname: Ong
authors.forename: H L
authors.initials: HL
authors.lastname: Cardenas
authors.forename: R
authors.initials: R
authors.lastname: Cavin
authors.forename: D L
authors.initials: DL
authors.lastname: Carnes
mesh_terms: D000469:Alkaline Phosphatase / Q000378:metabolism; D000818:Animals; D001672:Biocompatible Materials / Q000032:analysis / Q000737:chemistry*; D055396:Bone Morphogenetic Protein 2; D019485:Bone Morphogenetic Proteins / Q000494:pharmacology*; D002117:Calcitriol / Q000494:pharmacology; D002244:Carbon / Q000032:analysis; D002460:Cell Line; D004577:Electron Probe Microanalysis; D008422:Materials Testing; D051379:Mice; D009584:Nitrogen / Q000032:analysis; D010006:Osteoblasts / Q000166:cytology / Q000187:drug effects* / Q000201:enzymology; D015675:Osteocalcin / Q000096:biosynthesis / Q000187:drug effects; D010012:Osteogenesis; D010100:Oxygen / Q000032:analysis; D010641:Phenotype; D014176:Protein Biosynthesis; D011506:Proteins / Q000187:drug effects; D013234:Stem Cells / Q000166:cytology / Q000187:drug effects / Q000201:enzymology; D013499:Surface Properties; D014025:Titanium / Q000032:analysis / Q000737:chemistry*; D016212:Transforming Growth Factor beta / Q000494:pharmacology*
chemical_list: D001672:Biocompatible Materials; C525719:Bmp2 protein, mouse; D055396:Bone Morphogenetic Protein 2; D019485:Bone Morphogenetic Proteins; D011506:Proteins; D016212:Transforming Growth Factor beta; D015675:Osteocalcin; C009495:titanium dioxide; D002244:Carbon; D014025:Titanium; D000469:Alkaline Phosphatase; D002117:Calcitriol; D009584:Nitrogen; D010100:Oxygen