[Receptor mediated gene transfer in squamous epithelial carcinoma of the head-neck area].
BACKGROUND
The "Adeno-Virus-Enhanced-Receptor-Mediated" (AVET)-System uses the receptor-mediated endocytosis route to introduce DNA into mammalian cells. By the help of a replication defective adenovirus with endosomolytic activity the coentry of receptor-bound DNA particles is faciliated. The transport to the nucleus followed by gene expression is multifold enhanced.


METHODS
Two different permanent cell lines from head and neck squamous cell cancer were transfected with the reportergene for luciferase with either AVET or different liposoms in vitro.


RESULTS
In comparison it becomes obvious, that AVET shows a multifold higher transfection rate in vitro than the liposomal formulations applied.


CONCLUSIONS
AVET could be used for efficient transfection of epithelial cells, that are fairly reluctant to DNA transfer. Further trials in the mouse model must prove a possible application in vivo.
Wollenberg
B
B
Klinik und Poliklinik für Hals-Nasen-Ohren-Heilkunde und Kopf-Hals-Chirurgie, Ludwig-Maximilians-Universität München.
Schmidt
W
W
Schmitt
B
B
Lang
S
S
Zeidler
R
R
D000260:Adenoviruses, Human / Q000235:genetics*; D000818:Animals; D002294:Carcinoma, Squamous Cell / Q000235:genetics*; D002461:Cell Line, Transformed; D004705:Endocytosis / Q000235:genetics; D015967:Gene Expression Regulation, Viral / Q000502:physiology; D018014:Gene Transfer Techniques; D017930:Genes, Reporter / Q000235:genetics; D015316:Genetic Therapy / Q000379:methods; D006258:Head and Neck Neoplasms / Q000235:genetics*; D006801:Humans; D008081:Liposomes; D051379:Mice; D015398:Signal Transduction / Q000235:genetics*; D014162:Transfection / Q000235:genetics*
D008081:Liposomes