Effect of linoleic acid, linoleic acid anilide, and arachidonic acid on the expression of adhesion molecules on human neutrophils.
The effects of linoleic acid, linoleic acid anilide, and arachidonic acid on the expression of CD11b/ CD18, CD11c/CD18 integrins and L-selectin on human neutrophils were studied by flow cytometry in a whole blood assay. None of these compounds had any effect on the basal expression of CD11b, CD11c, or L-selectin in the concentration range of 20-100 microM. However, linoleic acid at a concentration of 1000 microM slightly up-regulated CD11b and CD11c by a factor of 2.1 and 1.7, respectively. Linoleic acid, linoleic acid anilide, and arachidonic acid did not affect the formyl-methionyl-leucyl-phenylalanine induced up-regulation of CD11b or CD11c. However, linoleic acid and linoleic acid anilide slightly inhibited the phorbol myristate acetate (PMA)-induced expression of CD11b, which was decreased by 27 and 21% at concentrations of 100 and 1000 microM, respectively. Likewise, arachidonic acid at 40 microM inhibited the PMA-induced expression of CD11b by 19%. Our results suggest that linoleic acid, linoleic acid anilide, and arachidonic acid do not dramatically affect the expression of leukocyte adhesion molecules in a whole blood assay.
Heiskanen
K M
KM
University of Kuopio, Department of Pharmacology and Toxicology, Finland.
Münzing
S
S
Krombach
F
F
Savolainen
K M
KM
D000813:Anilides / Q000633:toxicity*; D016718:Arachidonic Acid / Q000633:toxicity*; D018821:CD18 Antigens / Q000096:biosynthesis / Q000235:genetics; D015815:Cell Adhesion Molecules / Q000096:biosynthesis* / Q000235:genetics; D005434:Flow Cytometry; D006801:Humans; D016167:Integrin alphaXbeta2 / Q000096:biosynthesis / Q000235:genetics; D019041:L-Selectin / Q000096:biosynthesis / Q000235:genetics; D019787:Linoleic Acid / Q000633:toxicity*; D008041:Linoleic Acids / Q000633:toxicity*; D016177:Macrophage-1 Antigen / Q000096:biosynthesis / Q000235:genetics; D009240:N-Formylmethionine Leucyl-Phenylalanine; D009504:Neutrophils / Q000187:drug effects* / Q000378:metabolism; D016028:Receptors, Leukocyte-Adhesion / Q000096:biosynthesis* / Q000235:genetics; D013755:Tetradecanoylphorbol Acetate
D000813:Anilides; D018821:CD18 Antigens; D015815:Cell Adhesion Molecules; D016167:Integrin alphaXbeta2; D008041:Linoleic Acids; D016177:Macrophage-1 Antigen; D016028:Receptors, Leukocyte-Adhesion; D019041:L-Selectin; C039294:linoleylanilide; D016718:Arachidonic Acid; D009240:N-Formylmethionine Leucyl-Phenylalanine; D019787:Linoleic Acid; D013755:Tetradecanoylphorbol Acetate