title: Structural changes are associated with soluble N-ethylmaleimide-sensitive fusion protein attachment protein receptor complex formation.
abstract: SNAP-25, syntaxin, and synaptobrevin play a key role in the regulated exocytosis of synaptic vesicles, but their mechanism of action is not understood. In vitro, the proteins spontaneously assemble into a ternary complex that can be dissociated by the ATPase N-ethylmaleimide-sensitive fusion protein and the cofactors alpha-, beta-, and gamma-SNAP. Since the structural changes associated with these reactions probably form the basis of membrane fusion, we have embarked on biophysical studies aimed at elucidating such changes in vitro using recombinant proteins. All proteins were purified in a monomeric form. Syntaxin showed significant alpha-helicity, whereas SNAP-25 and synaptobrevin exhibited characteristics of largely unstructured proteins. Formation of the ternary complex induced dramatic increases in alpha-helicity and in thermal stability. This suggests that structure is induced in SNAP-25 and synaptobrevin upon complex formation. In addition, the stoichiometry changed from 2:1 in the syntaxin-SNAP-25 complex to 1:1:1 in the ternary complex. We propose that the transition from largely unstructured monomers to a tightly packed, energetically favored ternary complex connecting two membranes is a key step in overcoming energy barriers for membrane fusion.
authors.affiliation: Howard Hughes Medical Institute, New Haven, Connecticut 06510, USA.
authors.forename: D
authors.initials: D
authors.lastname: Fasshauer
authors.forename: H
authors.initials: H
authors.lastname: Otto
authors.forename: W K
authors.initials: WK
authors.lastname: Eliason
authors.forename: R
authors.initials: R
authors.lastname: Jahn
authors.forename: A T
authors.initials: AT
authors.lastname: Brünger
mesh_terms: D002352:Carrier Proteins / Q000737:chemistry / Q000378:metabolism*; D002850:Chromatography, Gel; D002942:Circular Dichroism; D008027:Light; D008565:Membrane Proteins / Q000737:chemistry / Q000378:metabolism*; D008970:Molecular Weight; D011487:Protein Conformation; D050767:Qc-SNARE Proteins; D012542:Scattering, Radiation; D033921:Vesicular Transport Proteins
chemical_list: C107588:BET1L protein, human; D002352:Carrier Proteins; D008565:Membrane Proteins; D050767:Qc-SNARE Proteins; D033921:Vesicular Transport Proteins