Anti-inflammatory activity of superoxide dismutase conjugated with sodium hyaluronate.
Superoxide dismutase (SOD) from bovine erythrocytes was conjugated with sodium hyaluronate (HA) with a mean molecular weight of 10(6) to have greater anti-inflammatory activity in vivo. Amino groups of SOD were coupled with carboxyl groups in the hyaluronate molecule using 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide. The HA-SOD conjugate was composed of 1.5 mol of SOD molecule per 1 mol of hyaluronate on the average, and retained 70% of the activity of unmodified SOD. The conjugate was essentially non-immunogenic in mice, and exhibited much higher anti-inflammatory activities than HA or SOD in models of inflammatory diseases such as ischemic oedema of the foot-pad in mice, carrageenin-induced pleurisy and adjuvant arthritis in rats.
Sakurai
K
K
Toin Human Science and Technology Centre, Department of Materials Science and Technology, Toin University of Yokohama, Japan.
Miyazaki
K
K
Kodera
Y
Y
Nishimura
H
H
Shingu
M
M
Inada
Y
Y
D000818:Animals; D000893:Anti-Inflammatory Agents / Q000737:chemistry / Q000494:pharmacology* / Q000627:therapeutic use; D001169:Arthritis, Experimental / Q000188:drug therapy; D002351:Carrageenan / Q000633:toxicity; D002417:Cattle; D005260:Female; D006820:Hyaluronic Acid / Q000737:chemistry*; D008297:Male; D051379:Mice; D010998:Pleurisy / Q000139:chemically induced / Q000188:drug therapy; D051381:Rats; D017207:Rats, Sprague-Dawley; D013482:Superoxide Dismutase / Q000737:chemistry*
D000893:Anti-Inflammatory Agents; D002351:Carrageenan; D006820:Hyaluronic Acid; D013482:Superoxide Dismutase