Phorbol esters alter the morphology of cultured guinea-pig myenteric glia via a protein kinase C-independent mechanism.
Cultures of myenteric ganglia from adult guinea-pigs were used to study the influence of neuroactive substances on glial cells by monitoring changes in their morphology. The following substances had no effect on glial morphology: adenosine, ATP, carbachol, glutamate, bradykinin, isoprenaline, prostaglandin E2, sodium nitroprusside and lipopolysaccharide. The only substances found to affect glial morphology were phorbol esters, and in particular phorbol 12-myrisate 13-acetate (PMA), which acted at the nM range. Glial cells, which were normally polygonal, assumed a stellate shape within 30-60 min after the addition of PMA. Protein kinase C (PKC) inhibitors did not block this effect, and PKC activators did not mimic it. The effect of PMA was also not mediated by changes in the intracellular concentrations of either Ca2+, H+ or cyclic AMP. Dye coupling among glial cells was blocked by PMA. The phorbol ester-mediated effect on glial structure may have profound influence on neuronal organization and function.
Hanani
M
M
Laboratory of Experimental Surgery, Hebrew University-Hadassah Medical School, Jerusalem, Israel. hananim@cc.huji.ac.il
Lin
Z
Z
Louzon
V
V
Brenner
T
T
Boneh
A
A
D000818:Animals; D002478:Cells, Cultured; D006168:Guinea Pigs; D009197:Myenteric Plexus / Q000166:cytology / Q000187:drug effects*; D009457:Neuroglia / Q000187:drug effects* / Q000201:enzymology; D010703:Phorbol Esters / Q000494:pharmacology*; D011493:Protein Kinase C / Q000378:metabolism*; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology*
D010703:Phorbol Esters; C008874:phorbol-12,13-diacetate; D011493:Protein Kinase C; D013755:Tetradecanoylphorbol Acetate