title: Mice lacking the thrombin receptor, PAR1, have normal skin wound healing.
abstract: Thrombin's actions on platelets, macrophages, fibroblasts, and endothelial cells have prompted the hypothesis that thrombin may be important for inflammatory and fibroproliferative processes in wound healing. Protease-activated receptor 1 (PAR1) is a G-protein-coupled receptor that mediates many of the cellular activities of thrombin. To test the role of this receptor in vivo, we generated PAR1-deficient mice. Despite the observation that fibroblasts cultured from these mice lacked responsiveness to thrombin in vitro, we now report that there was no difference detected between wild-type and PAR1-deficient mice in skin wound healing assays including time to closure of open wounds, tensile strength of healed incisional wounds, wound histology, and hydroxyproline/DNA content of wound implants. We conclude that PAR1 is not necessary for normal skin wound healing in mice.
authors.affiliation: Department of Pathology, University of California, San Francisco 94143-0130, USA.
authors.forename: A J
authors.initials: AJ
authors.lastname: Connolly
authors.forename: D Y
authors.initials: DY
authors.lastname: Suh
authors.forename: T K
authors.initials: TK
authors.lastname: Hunt
authors.forename: S R
authors.initials: SR
authors.lastname: Coughlin
mesh_terms: D000818:Animals; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D008815:Mice, Inbred Strains; D044463:Receptor, PAR-1; D018179:Receptors, Thrombin / Q000172:deficiency / Q000502:physiology*; D012016:Reference Values; D012867:Skin / Q000293:injuries* / Q000473:pathology; D013997:Time Factors; D014945:Wound Healing / Q000502:physiology*; D014950:Wounds, Penetrating / Q000378:metabolism / Q000473:pathology / Q000503:physiopathology*
chemical_list: D044463:Receptor, PAR-1; D018179:Receptors, Thrombin