Partial deletion of transposon Tn4560 integrated into the genome of Streptomyces tendae.
Polymerase chain reaction (PCR), Southern hybridization and DNA sequencing experiments were done to determine whether all of Tn4560, a Streptomyces transposon, integrated into the genomes of three nikkomycin non-producing mutants. A deletion of 279 bases occurred at one end of Tn4560 while present in the genome of one of the mutants.
Engel
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US Department of Agriculture, Agricultural Research Service, New Orleans, LA 70124-4305, USA.
Lax
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D000617:Aminoglycosides; D000900:Anti-Bacterial Agents / Q000096:biosynthesis; D001483:Base Sequence; D003001:Cloning, Molecular; D017931:DNA Primers / Q000235:genetics; D004251:DNA Transposable Elements / Q000235:genetics*; D004269:DNA, Bacterial / Q000235:genetics; D004926:Escherichia coli / Q000235:genetics; D005798:Genes, Bacterial; D016680:Genome, Bacterial; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D017384:Sequence Deletion; D013302:Streptomyces / Q000235:genetics* / Q000378:metabolism
D000617:Aminoglycosides; D000900:Anti-Bacterial Agents; D017931:DNA Primers; D004251:DNA Transposable Elements; D004269:DNA, Bacterial; C011952:nikkomycin