Determination of a new hypoxia selective agent from 2-quinoxalinecarbonitrile 1,4-di-N-oxides in plasma by high performance liquid chromatography.
A high performance liquid chromatography (HPLC) method was developed and validated for the determination of 7-chloro-3-[[(N,N-dimethylamino)propyl]amino]-2-quinaxolinecarbonitri le 1,4-di-N-oxide (Q-85), a new hypoxia-selective agent, in plasma. The assay involves extraction into diethylether-dichloromethane (2:1) from plasma and subsequent analysis by reversed-phase HPLC with ultraviolet detection at 292 nm. Response was linear in the range of 0.4-20 micrograms/ml of plasma and the detection limit was 0.05 micrograms/ml. The extraction recovery was about 90%. The within and between day coefficients of variation did not exceed 6.1%. This HPLC assay has been applied to determine Q-85 in plasma samples taken during pharmacokinetic studies in mice.
Zamalloa
E
E
Department of Medicinal Chemistry, University of Navarra, Pamplona, Spain.
Dios-Viéitez
C
C
González-Peñas
E
E
Monge
A
A
Aldana
I
I
D000818:Animals; D002138:Calibration; D002851:Chromatography, High Pressure Liquid; D005260:Female; D000860:Hypoxia / Q000378:metabolism*; D007275:Injections, Intravenous; D051379:Mice; D011810:Quinoxalines / Q000008:administration & dosage / Q000097:blood* / Q000493:pharmacokinetics; D013056:Spectrophotometry, Ultraviolet
C108265:7-chloro-3-(((N,N-dimethylamino)propyl)amino)-2-quinoxalinecarbonitrile-1,4-di-N-oxide; D011810:Quinoxalines