TSC-36 (follistatin-related polypeptide) gene expression in estrogen receptor positive osteoblastic cell line, CDO7F.
Using an osteoblastic cell line (CDO7F) that was selected as an estrogen receptor positive cell by immunocytochemical detection and reverse transcription-polymerase chain reaction (RT-PCR) subtractive cDNA cloning using oligo(dT)30-Latex and PCR was performed to isolate a gene that is induced by estrogen. A cDNA clone was isolated and its nucleotide sequence identified it as TSC 36, previously called follistatin-related polypeptide. Northern blot analysis showed that the TSC-36 gene expression was certainly upregulated by estrogen. Tamoxifen also upregulated this gene expression. These findings indicate that TSC-36 may be one of the estrogen-regulated genes in osteoblastic cells.
Ohashi
T
T
Laboratory of Cell Biology, Tsukuba Life Science Center, Institute of Physical and Chemical Research (RIKEN), Ibaraki, Japan.
Sato
S
S
Yoshiki
A
A
Kusakabe
M
M
D000818:Animals; D002460:Cell Line; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics; D004965:Estrogen Antagonists / Q000494:pharmacology; D004967:Estrogens / Q000494:pharmacology; D038702:Follistatin-Related Proteins; D005786:Gene Expression Regulation / Q000235:genetics*; D006023:Glycoproteins / Q000096:biosynthesis / Q000235:genetics*; D051379:Mice; D010006:Osteoblasts / Q000187:drug effects* / Q000378:metabolism; D011960:Receptors, Estrogen / Q000235:genetics* / Q000378:metabolism; D013629:Tamoxifen / Q000494:pharmacology; D015854:Up-Regulation
D018076:DNA, Complementary; D004965:Estrogen Antagonists; D004967:Estrogens; D038702:Follistatin-Related Proteins; D006023:Glycoproteins; D011960:Receptors, Estrogen; D013629:Tamoxifen