Difference in HMG1-induced DNA bending among microsatellites.
Sequence-dependency of high-mobility group protein (HMG) 1-induced DNA bending is examined for microsatellites using a circularization assay which can measure the extent of bending. Fragments of 133 bp containing (GGA/TCC)11 in the middle showed greater bending than those harboring (GAA/TTC)11 and (GT/AC)17 repeats, and fragments possessing (GA/TC)17 exhibited only slight bending. Differences were not detected for fragments having the repeats near the end. Filter binding assays showed no difference in their binding affinity, suggesting that GGA/TCC repeats are more flexible than the other three repeats as concerns HMG1-induced bending. These results suggest that the mammalian genomes comprise flexible and inflexible regions of microsatellites which might play roles in chromatin architectures and in dynamic packaging of genomic DNA during the cell division cycle.
Takayanagi
K
K
First Department of Biochemistry, Niigata University School of Medicine, Japan.
Mishima
Y
Y
Kominami
R
R
D002843:Chromatin / Q000737:chemistry; D004247:DNA / Q000737:chemistry*; D018900:Dinucleotide Repeats; D006609:High Mobility Group Proteins / Q000235:genetics*; D018895:Microsatellite Repeats / Q000235:genetics*; D009690:Nucleic Acid Conformation; D018911:Trinucleotide Repeats
D002843:Chromatin; D006609:High Mobility Group Proteins; D004247:DNA