Influenza virus NS1 protein interacts with viral transcription-replication complexes in vivo.
The interaction of influenza virus NS1 protein with other viral products in the infected cell was analysed by co-immunoprecipitation studies. The three subunits of the polymerase and the nucleoprotein, but not M1 protein, were co-immunoprecipitated by NS1-specific serum but not when control serum was used. Such co-immunoprecipitation was not sensitive to RNase treatment of the immunoprecipitates. Co-immunoprecipitation was also obtained when the viral transcription-replication system was reconstituted in vivo by transfection of cDNAs and model vRNA template into vaccinia virus-T7-infected cells. Analysis of the RNA pulled-down in the NS1-specific precipitates indicated the presence of both vRNA and mRNA. These results are discussed in the context of the phenotype of virus temperature-sensitive mutants affected in the NS1 gene.
Marión
R M
RM
Centro Nacional de Biotecnología (CSIC), Campus de Cantoblanco, Madrid, Spain.
Zürcher
T
T
de la Luna
S
S
Ortín
J
J
D000818:Animals; D019556:COS Cells; D015967:Gene Expression Regulation, Viral; D009980:Influenza A virus / Q000235:genetics*; D046911:Macromolecular Substances; D019590:Nucleocapsid Proteins; D009698:Nucleoproteins; D011233:Precipitin Tests; D012367:RNA, Viral / Q000378:metabolism; D016601:RNA-Binding Proteins / Q000378:metabolism; D014158:Transcription, Genetic; D014758:Viral Core Proteins / Q000378:metabolism; D017361:Viral Nonstructural Proteins / Q000378:metabolism*; D014764:Viral Proteins / Q000378:metabolism; D014779:Virus Replication
C085820:INS1 protein, influenza virus; D046911:Macromolecular Substances; D019590:Nucleocapsid Proteins; D009698:Nucleoproteins; D012367:RNA, Viral; D016601:RNA-Binding Proteins; D014758:Viral Core Proteins; D017361:Viral Nonstructural Proteins; D014764:Viral Proteins; C077346:influenza virus polymerase basic protein 1