PCR amplification of ribosomal DNA spacer regions of Salmonella enteritidis isolates.
Polymerase chain reaction (PCR) was used to amplify ribosomal DNA spacer regions from nine Salmonella enteritidis field isolates. Unique products of 480 and 660 bp were obtained from the isolates. PCR product (480bp) was then cloned into pUC18 vector by blunt end ligation.
Chaudhuri
P
P
National Biotechnology Centre, Indian Veterinary Research Institute, Izatnagar, India.
Singh
V P
VP
Sharma
B
B
D004269:DNA, Bacterial / Q000235:genetics*; D004275:DNA, Ribosomal / Q000235:genetics*; D016133:Polymerase Chain Reaction; D012475:Salmonella / Q000235:genetics*
D004269:DNA, Bacterial; D004275:DNA, Ribosomal