Refined subchromosomal location of 21 expressed sequence tags from unknown genes at region 11p15.
Twenty-one expressed sequence tags (ESTs) belonging to unidentified transcripts were mapped to 9 intervals at 11p 15.5-p15.3. Thirteen were mapped to a region of an estimated size of 7 Mb with the help of a YAC contig. The remaining eight were mapped outside this region, and the centromeric or telomeric position of the ESTs relative to the YAC contig was defined with the help of a cellular hybrid containing a derivative chromosome 11 with a break-point within the region covered by the contig. The average size of the intervals, where the ESTs were mapped, was estimated to be 0.3-0.4 Mb. The refinement of the chromosomal location of these ESTs could be of great help in the identification of potential candidate genes for disease locus mapping at 11p15.
Schwienbacher
C
C
Department of Experimental and Diagnostic Medicine, University of Ferrara, Italy.
Sabbioni
S
S
Barbanti-Brodano
G
G
Negrini
M
M
D000818:Animals; D002874:Chromosome Mapping; D002880:Chromosomes, Human, Pair 11; D018076:DNA, Complementary; D015870:Gene Expression; D006801:Humans; D019656:Loss of Heterozygosity; D051379:Mice
D018076:DNA, Complementary