title: An operational amplifier B1404UD1A-1 in the patch-clamp current-to-voltage converter.
abstract: The applicability of the home-made operational amplifier B1404UD1A-1 in a patch-clamp current-to-voltage converter was analyzed. Its parameters (background noise, input bias current, and gain-bandwidth product) were estimated. Schematic solutions and practical recommendations for the use of this amplifier in a current-to-voltage converter were given. Based on the background noise and frequency parameters of the converter, we found that this device can be used for measuring ion channel currents with a high sensitivity and within a broad frequency range (0.055 pA, to 1 kHz; 0.4 pA, to 10 kHz). An example of the converter application in experiments is given.
authors.affiliation: Siberian Institute of Plant Physiology and Biochemistry, Siberian Branch of the Russian Academy of Sciences, Irkutsk.
authors.forename: A M
authors.initials: AM
authors.lastname: Korzun
authors.forename: S V
authors.initials: SV
authors.lastname: Rozinov
authors.forename: G I
authors.initials: GI
authors.lastname: Abashin
mesh_terms: D000669:Amplifiers, Electronic; D016477:Artifacts; D004553:Electric Conductivity; D017097:Electric Impedance; D004581:Electronics / Q000295:instrumentation; D005069:Evaluation Studies as Topic; D018408:Patch-Clamp Techniques / Q000295:instrumentation*; D012680:Sensitivity and Specificity