title: The Escherichia coli ldcC gene encodes another lysine decarboxylase, probably a constitutive enzyme.
abstract: A gene (designated ldcC) mapped at 4.6 min on the Escherichia coli chromosome codes for a protein of 713 amino acids (aa) that shows strong similarities in both size and amino-acid sequence (69% identical residues and 85% conserved residues) to lysine decarboxylase (LDC) from E. coli (CadA, acid-inducible LDC, 715 aa) or from Hafnia alvei (739 aa). A pUC18 derivative carrying the ldcC gene conferred high LDC activities on an E. coli strain devoid of the functional cadA gene, even when the bacteria were grown under non-inducing conditions at physiological pH. Thus, the gene encodes another lysine decarboxylase, probably a constitutively expressed enzyme, the presence of which was suggested from the previous observations that low LDC activities were detectable in cadA- mutant and non-induced wild-type cells.
authors.affiliation: Department of Genetics, Hyogo College of Medicine, Japan.
authors.forename: Y
authors.initials: Y
authors.lastname: Yamamoto
authors.forename: Y
authors.initials: Y
authors.lastname: Miwa
authors.forename: K
authors.initials: K
authors.lastname: Miyoshi
authors.forename: J
authors.initials: J
authors.lastname: Furuyama
authors.forename: H
authors.initials: H
authors.lastname: Ohmori
mesh_terms: D001426:Bacterial Proteins / Q000235:genetics; D001483:Base Sequence; D002262:Carboxy-Lyases / Q000235:genetics*; D004269:DNA, Bacterial / Q000235:genetics; D004926:Escherichia coli / Q000201:enzymology / Q000235:genetics*; D015964:Gene Expression Regulation, Bacterial; D005798:Genes, Bacterial; D005816:Genetic Complementation Test; D008969:Molecular Sequence Data; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid
chemical_list: D001426:Bacterial Proteins; D004269:DNA, Bacterial; D002262:Carboxy-Lyases; C021520:lysine decarboxylase