title: MIB-1 and S-phase cell fraction predict survival in non-Hodgkin's lymphomas.
abstract: The monoclonal antibody anti-Ki67 is used to detect proliferating cells, but its main limitation is the requirement of fresh-frozen material. On a series of patients with non-Hodgkin's lymphoma, we used a Ki67 equivalent monoclonal antibody, the recently proposed MIB-1, on formalin-fixed histopathological material using microwave antigen retrieval. MIB-1 expression was analysed in relation to other proliferation indices, such as autoradiographic H-thymidine labelling index (HTL1) and flow cytometric S-phase cell fraction (FCM-S) and to pathological status. Moreover, the prognostic relevance of the cell kinetic indices was defined in uni- and multivariate analyses including histology and tumour stage. The relationship between MIB-1 index and the other proliferation indices was statistically significant even though the correlation coefficient was around 0.6. The MIB-1 index was also related to the REAL (Revised European American Lymphoma) classification, but not to the Ann Arbor stage classification. Univariate analysis showed that the MIB-1 index was a significant predictor of 6-year survival in the overall series and in distinctly analysed low-grade and high-grade lymphoma subgroups. With regard to S-phase indices, HTLI was a powerful prognosticator in patients with high-grade histologies and FCM-S in patients with low-grade histologies. Multivariate analyses revealed that MIB-1 index, HTLI and FCM-S retained their prognostic significance independent of histology. In conclusion, the MIB-1 antibody provides prognostic information in non-Hodgkin's lymphomas and has the main advantage that it can be used in formalin-fixed, paraffin-embedded specimens.
authors.affiliation: Division of Oncologia Sperimentale C and Anatomia Patologica e Citopatologia, Istituto Nazionale per lo Studio e la Cura dei Tumori, Milan, Italy.
authors.forename: C
authors.initials: C
authors.lastname: Mochen
authors.forename: R
authors.initials: R
authors.lastname: Giardini
authors.forename: A
authors.initials: A
authors.lastname: Costa
authors.forename: R
authors.initials: R
authors.lastname: Silvestrini
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000911:Antibodies, Monoclonal; D005260:Female; D006801:Humans; D019394:Ki-67 Antigen / Q000032:analysis; D008228:Lymphoma, Non-Hodgkin / Q000175:diagnosis* / Q000473:pathology*; D008297:Male; D008875:Middle Aged; D015999:Multivariate Analysis; D011379:Prognosis; D012044:Regression Analysis; D016196:S Phase; D016019:Survival Analysis
chemical_list: D000911:Antibodies, Monoclonal; D019394:Ki-67 Antigen