Identification and cloning of differentially expressed genes.
Only a few of the methods currently used for identification of differentially expressed genes take advantage of the fact that (near) complete sets of cDNA clones and sequences representing all human and mouse genes will be available for high throughput survey of gene expression. Accordingly, strategies based on hybridization of complex (cDNA or RNA) probes to cDNA microarrays, either on glass slides or on chips, are likely to become increasingly more advantageous. Recognizing, however, that the power of these methods depends upon the availability of such resources, strategies are being pursued to facilitate completion of the ongoing efforts to identify all human and mouse genes.
Soares
M B
MB
Department of Pediatrics, University of Iowa, Iowa City 52245, USA. msoares@blue.weeg.uiowa.edu
D000818:Animals; D019976:Cloning, Organism / Q000379:methods*; D018076:DNA, Complementary; D015870:Gene Expression; D015723:Gene Library; D006801:Humans; D051379:Mice; D016133:Polymerase Chain Reaction / Q000379:methods; D015347:RNA Probes
D018076:DNA, Complementary; D015347:RNA Probes