title: In vitro transcription systems from cultured cells and fat-body tissue of the silkworm, Bombyx mori.
abstract: This article describes the development of cell-free transcription systems from the cultured cells and fat body tissues of a Lepidpteran insect, the silkworm, Bombyx mori. Detailed protocols are provided for the culture of a B. mori cell line, rearing larvae, preparation of whole cell as well as nuclear extract and conditions for in vitro transcription of cloned plasma protein gene templates.
authors.affiliation: Department of Biology, Tokyo Metropolitan University, Japan.
authors.forename: E
authors.initials: E
authors.lastname: Mine
authors.forename: H
authors.initials: H
authors.lastname: Sakurai
authors.forename: S
authors.initials: S
authors.lastname: Izumi
authors.forename: S
authors.initials: S
authors.lastname: Tomino
mesh_terms: D000818:Animals; D012831:Bombyx / Q000378:metabolism*; D002474:Cell-Free System; D002478:Cells, Cultured; D005216:Fat Body / Q000378:metabolism*; D005260:Female; D005821:Genetic Techniques; D007202:Indicators and Reagents; D010053:Ovary / Q000166:cytology / Q000378:metabolism; D014158:Transcription, Genetic
chemical_list: D007202:Indicators and Reagents