title: Involvement of a 43-kilodalton outer membrane protein in beta-lactam resistance of Shigella dysenteriae.
abstract: A beta-lactam-sensitive strain (C152) of Shigella dysenteriae showed two major outer membrane proteins (OMPs) with M(r)s of 43,000 and 38,000, while the clinical isolate M2 lacked the 43,000-Mr OMP, which acted as a channel for beta-lactam antibiotics. Permeability of beta-lactams across the outer membrane (OM) of M2 was lower than that across the OM of C152. Mutants deficient in the 43-kDa OMP could be selected in vitro from strain C152 in the presence of cefoxitin. All beta-lactam-resistant strains were sensitive to imipenem.
authors.affiliation: Department of Chemistry, Bose Institute, Calcutta, India.
authors.forename: A K
authors.initials: AK
authors.lastname: Kar
authors.forename: A S
authors.initials: AS
authors.lastname: Ghosh
authors.forename: K
authors.initials: K
authors.lastname: Chauhan
authors.forename: J
authors.initials: J
authors.lastname: Ahamed
authors.forename: J
authors.initials: J
authors.lastname: Basu
authors.forename: P
authors.initials: P
authors.lastname: Chakrabarti
authors.forename: M
authors.initials: M
authors.lastname: Kundu
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology; D001425:Bacterial Outer Membrane Proteins / Q000032:analysis / Q000235:genetics / Q000502:physiology*; D008826:Microbial Sensitivity Tests; D008970:Molecular Weight; D009154:Mutation; D012762:Shigella dysenteriae / Q000187:drug effects* / Q000201:enzymology / Q000235:genetics; D018440:beta-Lactam Resistance / Q000235:genetics / Q000502:physiology*; D001618:beta-Lactamases / Q000378:metabolism; D047090:beta-Lactams
chemical_list: D000900:Anti-Bacterial Agents; D001425:Bacterial Outer Membrane Proteins; D047090:beta-Lactams; D001618:beta-Lactamases