Derivatization of amphetamine and methamphetamine with 1,2-naphthoquinone 4-sulfonic acid into solid-phase extraction cartridges. Determination of amphetamine in pharmaceutical and urine samples.
The derivatization of amphetamine and methamphetamine with 1,2-naphthoquinone-4-sulfonate (NQS) into solid-phase extraction cartridges (C18) is described. Optimum conditions were the use of carbonate-hydrogencarbonate buffer of pH 10, a 10 min reaction time at 25 degrees C and an NQS concentration of 9.6 x 10(-3) M. The accuracy and the precision of the method were tested. The results obtained with the proposed liquid-solid procedure were compared with those obtained with a traditional liquid-liquid extraction with hexane-ethyl acetate. The procedure was used to measure amphetamine in pharmaceutical and urine samples.
Campíns Falcó
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Departamento de Química Analítica, Facultad de Química, Universidad de Valencia, Burjassot, Spain.
Molins Legua
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Sevillano Cabeza
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A
Porras Serrano
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R
D000661:Amphetamine / Q000032:analysis* / Q000652:urine; D000697:Central Nervous System Stimulants / Q000032:analysis* / Q000652:urine; D006801:Humans; D008694:Methamphetamine / Q000032:analysis / Q000652:urine; D009285:Naphthoquinones; D004364:Pharmaceutical Preparations / Q000737:chemistry*; D013056:Spectrophotometry, Ultraviolet; D013451:Sulfonic Acids
D000697:Central Nervous System Stimulants; D009285:Naphthoquinones; D004364:Pharmaceutical Preparations; D013451:Sulfonic Acids; D008694:Methamphetamine; C040756:1,2-naphthoquinone; D000661:Amphetamine