title: Large scale identification of genes involved in cell surface biosynthesis and architecture in Saccharomyces cerevisiae.
abstract: The sequenced yeast genome offers a unique resource for the analysis of eukaryotic cell function and enables genome-wide screens for genes involved in cellular processes. We have identified genes involved in cell surface assembly by screening transposon-mutagenized cells for altered sensitivity to calcofluor white, followed by supplementary screens to further characterize mutant phenotypes. The mutated genes were directly retrieved from genomic DNA and then matched uniquely to a gene in the yeast genome database. Eighty-two genes with apparent perturbation of the cell surface were identified, with mutations in 65 of them displaying at least one further cell surface phenotype in addition to their modified sensitivity to calcofluor. Fifty of these genes were previously known, 17 encoded proteins whose function could be anticipated through sequence homology or previously recognized phenotypes and 15 genes had no previously known phenotype.
authors.affiliation: Department of Biology, McGill University, Montréal, Québec, Canada.
authors.forename: M
authors.initials: M
authors.lastname: Lussier
authors.forename: A M
authors.initials: AM
authors.lastname: White
authors.forename: J
authors.initials: J
authors.lastname: Sheraton
authors.forename: T
authors.initials: T
authors.lastname: di Paolo
authors.forename: J
authors.initials: J
authors.lastname: Treadwell
authors.forename: S B
authors.initials: SB
authors.lastname: Southard
authors.forename: C I
authors.initials: CI
authors.lastname: Horenstein
authors.forename: J
authors.initials: J
authors.lastname: Chen-Weiner
authors.forename: A F
authors.initials: AF
authors.lastname: Ram
authors.forename: J C
authors.initials: JC
authors.lastname: Kapteyn
authors.forename: T W
authors.initials: TW
authors.lastname: Roemer
authors.forename: D H
authors.initials: DH
authors.lastname: Vo
authors.forename: D C
authors.initials: DC
authors.lastname: Bondoc
authors.forename: J
authors.initials: J
authors.lastname: Hall
authors.forename: W W
authors.initials: WW
authors.lastname: Zhong
authors.forename: A M
authors.initials: AM
authors.lastname: Sdicu
authors.forename: J
authors.initials: J
authors.lastname: Davies
authors.forename: F M
authors.initials: FM
authors.lastname: Klis
authors.forename: P W
authors.initials: PW
authors.lastname: Robbins
authors.forename: H
authors.initials: H
authors.lastname: Bussey
mesh_terms: D002462:Cell Membrane / Q000378:metabolism; D004251:DNA Transposable Elements; D005800:Genes, Fungal; D008826:Microbial Sensitivity Tests; D016297:Mutagenesis, Site-Directed; D010641:Phenotype; D012441:Saccharomyces cerevisiae / Q000235:genetics*
chemical_list: D004251:DNA Transposable Elements