Somatic mutations of PTEN in glioblastoma multiforme.
Alterations of the PTEN gene occur in glioblastoma multiforme. To determine the frequency of PTEN alteration, 34 consecutive glioblastomas were studied in detail. Sequencing each of the nine exons amplified from tumor DNA revealed 11 mutations. Analysis of polymorphic markers within and surrounding the PTEN gene identified an additional four homozygous deletion mutations. Loss of heterozygosity (LOH) was observed in 25 of 34 (74%) cases. All mutations occurred in the presence of LOH. PTEN was mutated in 44% (15 of 34) of all glioblastomas studied and 60% (15 of 25) of tumors with LOH on 10q. Thus, PTEN appears to be the major target of inactivation on chromosome 10q in glioblastoma multiforme.
Wang
S I
SI
Department of Pathology and Medicine, College of Physicians and Surgeons, Columbia University, New York, New York 10032, USA.
Puc
J
J
Li
J
J
Bruce
J N
JN
Cairns
P
P
Sidransky
D
D
Parsons
R
R
D001254:Astrocytoma / Q000235:genetics / Q000473:pathology; D001932:Brain Neoplasms / Q000235:genetics*; D002879:Chromosomes, Human, Pair 10 / Q000235:genetics*; D056741:Cyclin D; D016213:Cyclins / Q000502:physiology; D004252:DNA Mutational Analysis; D004273:DNA, Neoplasm / Q000235:genetics; D018450:Disease Progression; D066246:ErbB Receptors / Q000235:genetics; D005091:Exons / Q000235:genetics; D016147:Genes, Tumor Suppressor; D016158:Genes, p53; D005909:Glioblastoma / Q000235:genetics*; D006801:Humans; D019656:Loss of Heterozygosity; D017027:Protein Tyrosine Phosphatases / Q000235:genetics*; D017384:Sequence Deletion
D056741:Cyclin D; D016213:Cyclins; D004273:DNA, Neoplasm; D066246:ErbB Receptors; D017027:Protein Tyrosine Phosphatases