Comparison of different selective methods for detection of Listeria species in surface water.
Tests were carried out to evaluate the efficiency of various combinations of selective enrichment and plating techniques using pure cultures of Listeria monocytogenes, Listeria seeligeri and Listeria innocua in suspension and samples of surface water. The best yields for the various Listeria spp. were obtained after a single passage in LEB (Oxoid) or LEB Buffered (Oxoid) and after using Palcam and Oxford agar (Oxoid). Palcam agar was, however, shown to be the most efficient means of detecting the Listeria spp. in the natural water samples.
Bernagozzi
M
M
Università degli Studi di Bologna, Facoltà di Medicina e Chiruriga, IIo Cattedra di Igiene.
Sacchetti
R
R
Polenta
L
L
Bisbini
P
P
D000362:Agar; D001431:Bacteriological Techniques; D003470:Culture Media; D008087:Listeria / Q000145:classification / Q000254:growth & development / Q000302:isolation & purification*; D008089:Listeria monocytogenes / Q000145:classification / Q000254:growth & development / Q000302:isolation & purification; D012703:Serotyping; D014871:Water Microbiology
D003470:Culture Media; D000362:Agar