Use of a newly developed rapid microbial ATP bioluminescence assay to detect microbial contamination on poultry carcasses.
A newly developed rapid microbial ATP bioluminescence test (R-mATP) was shown to be an adequate means to assay the microbial load of poultry carcasses. This assay utilizes differential extraction and filtration to separate somatic from microbial ATP in a very rapid timeframe. The assay requires approximately 5 min to complete; approximately 3.5 min to sample and 90 s analytical time. Correlation coefficient (r) between aerobic colony counts and R-mATP test results (n=329) was 0.82. Post-test probabilities to correctly classify carcasses with different levels of microbial contamination were as high as 98% for samples of > or = 3.5 log aerobic CFU per ml. Given the rapidity of this assay, the R-mATP holds potential for monitoring the microbial load of carcasses at poultry-processing critical control points. Other potential applications of this new version of the microbial ATP bioluminescence test are discussed.
Siragusa
G R
GR
United States Department of Agriculture, Agricultural Research Service (USDA-ARS), Roman L. Hruska US Meat Animal Research Center, Clay Center, NE 68933, USA.
Dorsa
W J
WJ
Cutter
C N
CN
Perino
L J
LJ
Koohmaraie
M
M
D000255:Adenosine Triphosphate / Q000032:analysis*; D000818:Animals; D001699:Biometry; D015169:Colony Count, Microbial; D005069:Evaluation Studies as Topic; D008163:Luminescent Measurements; D008828:Microbiological Techniques / Q000706:statistics & numerical data; D011200:Poultry / Q000382:microbiology*; D013997:Time Factors
D000255:Adenosine Triphosphate