title: HIV-1 capsid mutants inhibit the replication of wild-type virus at both early and late infection phases.
abstract: In-frame mutations were introduced into various portions of the human immunodeficiency virus type 1 (HIV-1) gag gene, and potentials of the mutants to suppress the replication of wild-type HIV-1 were monitored. In contrast to results obtained with matrix and nucleocapsid mutants, almost all capsid mutants blocked HIV-1 replication completely in single-round replication assays. A capsid mutant designated C6b was demonstrated to be one of the most efficient inhibitors for HIV-1 reported to date, and to be effective at both early and late viral replication phases. T-cells, which are engineered to express the C6b Gag in response to HIV-1 infection, were perfectly resistant to HIV-1.
authors.affiliation: Institute for Virus Research, Kyoto University, Japan.
authors.forename: R A
authors.initials: RA
authors.lastname: Furuta
authors.forename: R
authors.initials: R
authors.lastname: Shimano
authors.forename: T
authors.initials: T
authors.lastname: Ogasawara
authors.forename: R
authors.initials: R
authors.lastname: Inubushi
authors.forename: K
authors.initials: K
authors.lastname: Amano
authors.forename: H
authors.initials: H
authors.lastname: Akari
authors.forename: M
authors.initials: M
authors.lastname: Hatanaka
authors.forename: M
authors.initials: M
authors.lastname: Kawamura
authors.forename: A
authors.initials: A
authors.lastname: Adachi
mesh_terms: D015153:Blotting, Western; D015496:CD4-Positive T-Lymphocytes / Q000821:virology; D002213:Capsid / Q000737:chemistry / Q000235:genetics* / Q000502:physiology; D015500:Chloramphenicol O-Acetyltransferase / Q000235:genetics / Q000378:metabolism; D015870:Gene Expression; D015683:Gene Products, gag / Q000235:genetics / Q000502:physiology*; D015750:Genes, gag; D005822:Genetic Vectors; D015497:HIV-1 / Q000235:genetics* / Q000502:physiology*; D006801:Humans; D016296:Mutagenesis; D009154:Mutation; D014162:Transfection; D014407:Tumor Cells, Cultured; D014779:Virus Replication
chemical_list: D015683:Gene Products, gag; D015500:Chloramphenicol O-Acetyltransferase