Encapsidation of potyviral RNA in various forms of transgene coat protein is not correlated with resistance in transgenic plants.
Transgenic plants expressing either bean yellow mosaic potyvirus or chimeric potyvirus coat protein (CP) were inoculated with various potyviruses. Antigen-coated plate, indirect enzyme-linked immunosorbent assay and immunoelectron microscopy of virus purified from transgenic plants showed that progeny virions contained from < 1% to as much as 25% transgenic CP. Different levels of transcapsidation may reflect the extent of compatibility between transgene CP and the viral CP.
Hammond
J
J
United States Department of Agriculture, Agricultural Research Service, United States National Arboretum, Beltsville, MD 20705, USA. jhammond@asrr.arsusda.gov
Dienelt
M M
MM
D000595:Amino Acid Sequence; D000818:Animals; D001042:Aphids; D002213:Capsid / Q000235:genetics* / Q000378:metabolism; D007303:Insect Vectors; D008969:Molecular Sequence Data; D010935:Plant Diseases / Q000821:virology*; D030821:Plants, Genetically Modified; D010947:Plants, Toxic; D017800:Potyvirus / Q000235:genetics / Q000254:growth & development* / Q000648:ultrastructure; D012367:RNA, Viral / Q000378:metabolism; D011994:Recombinant Proteins / Q000378:metabolism; D013045:Species Specificity; D014026:Nicotiana / Q000235:genetics / Q000821:virology
D012367:RNA, Viral; D011994:Recombinant Proteins