Isolation, characterization and molecular cloning of a leaf-specific lectin from ramsons (Allium ursinum L.).
Lectins were isolated from roots and leaves of ramsons and compared to the previously described bulb lectins. Biochemical analyses indicated that the root lectins AUAIr and AUAIIr are identical to the bulb lectins AUAI and AUAII, whereas the leaf lectin AUAL has no counterpart in the bulbs. cDNA cloning confirmed that the leaf lectin differs from the bulb lectins. Northern blot analysis further indicated that the leaf lectin is tissue-specifically expressed. Sequence comparisons revealed that the ramsons leaf lectin differs considerably from the leaf lectins of garlic, leek, onion and shallot.
Smeets
K
K
Laboratory for Phytopathology and Plant Protection, Katholieke Universiteit Leuven, Belgium.
Van Damme
E J
EJ
Van Leuven
F
F
Peumans
W J
WJ
D000490:Allium / Q000737:chemistry* / Q000235:genetics; D000595:Amino Acid Sequence; D002460:Cell Line; D003001:Cloning, Molecular; D003588:Cytopathogenic Effect, Viral; D018076:DNA, Complementary / Q000235:genetics; D018744:DNA, Plant / Q000235:genetics; D015497:HIV-1 / Q000502:physiology; D015498:HIV-2 / Q000502:physiology; D006386:Hemagglutination Tests; D006801:Humans; D037102:Lectins / Q000737:chemistry / Q000235:genetics* / Q000302:isolation & purification* / Q000494:pharmacology; D008969:Molecular Sequence Data; D008970:Molecular Weight; D010802:Phylogeny; D018515:Plant Leaves / Q000737:chemistry; D037121:Plant Lectins; D012333:RNA, Messenger / Q000032:analysis; D018749:RNA, Plant / Q000032:analysis; D013601:T-Lymphocytes
D018076:DNA, Complementary; D018744:DNA, Plant; D037102:Lectins; D037121:Plant Lectins; D012333:RNA, Messenger; D018749:RNA, Plant