A universal post-embedding protocol for immunogold labelling of osmium-fixed, epoxy resin-embedded tissue.
The authors have found that double etching of epoxy-embedded, ultrathin sections with 3% sodium metaperiodate rendered epitope expression comparable to that obtained with either saturated or half-saturated sodium metaperiodate solutions. In contrast to either of the two more concentrated oxidizing solutions, double etching with 3% sodium metaperiodate neither bleached the specimens nor generated holes in the plastic resin.
Morris
R E
RE
Department of Cell Biology, Neurobiology and Anatomy, College of Medicine, University of Cincinnati, OH 45267, USA.
Ciraolo
G M
GM
D000818:Animals; D004853:Epoxy Resins; D005069:Evaluation Studies as Topic; D006658:Histoplasma / Q000648:ultrastructure; D008168:Lung / Q000648:ultrastructure; D016253:Microscopy, Immunoelectron / Q000379:methods*; D009993:Osmium Tetroxide; D010504:Periodic Acid; D051381:Rats; D013211:Staphylococcus aureus / Q000648:ultrastructure; D016610:Tissue Embedding / Q000379:methods*; D016707:Tissue Fixation / Q000379:methods
D004853:Epoxy Resins; D010504:Periodic Acid; C009288:metaperiodate; D009993:Osmium Tetroxide