Measurement of bilirubin by HPLC in bovine plasma--a comparison with the conventional diazo-method.
Application of a HPLC-technique according to MURACA & BLANCKAERT (1983) for accurate determination of the relative amounts of unconjugated bilirubin and its sugar mono- and diconjugates in bovine plasma is described and the results are compared with those of the conventional diazo-method. The diazo-assay yielded values for total and unconjugated bilirubin which were considerably higher than those obtained by the HPLC-technique. This is probably due to unidentified diazo-positive compounds distinct from bilirubin. The direct-reacting fraction in the diazo-assay showed little or no agreement with the fraction of total ester conjugates determined by HPLC. Results indicate that the diagnostical value of bilirubin subfractioning by the diazo-method as a liver function test in cattle must be reconsidered.
Qualmann
K
K
Clinic for Cattle, School of Veterinary Medicine, Hannover.
Rehage
J
J
Scholz
H
H
D000818:Animals; D001391:Azo Compounds; D001663:Bilirubin / Q000097:blood*; D002417:Cattle; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D005260:Female; D008111:Liver Function Tests / Q000379:methods / Q000662:veterinary; D012044:Regression Analysis; D013053:Spectrophotometry / Q000379:methods
D001391:Azo Compounds; D001663:Bilirubin