Cytokine regulation of human immune response to Schistosoma mansoni: analysis of the role of IL-4, IL-5 and IL-10 on peripheral blood mononuclear cell responses.
The role of cytokines on the in vitro proliferative response of peripheral blood mononuclear cells (PBMC) from Schistosoma mansoni infected patients to soluble egg (SEA) and adult worm antigens (SWAP) were evaluated. The results obtained demonstrated that the proliferative response of PBMC from chronic intestinal (INT) patients to SEA and SWAP is increased by the blockage of IL-10 with specific monoclonal antibodies (MAb). The effects of these antibodies were readily reversed by the addition of recombinant IL-10. In contrast, no effect was observed on the PBMC response of acute and hepatosplenic patients (HS) in the presence of anti-IL-10. Anti-IL-4 antibodies decreased the PBMC response of the intestinal (INT) and HS individuals to SEA and SWAP, and the PBMC response of acute patients to SEA but not to SWAP. Addition of anti-IL-5 MAb did not decrease the PBMC response of acute patients to SEA or SWAP. These results suggested that IL-10 has an important role in the modulation of the immune response in chronic asymptomatic patients and that this cytokine may be an important factor in controlling morbidity.
Malaquias
L C
LC
Centro de Pesquisas René Rachou-FIOCRUZ, Belo Horizonte, Brazil.
Falcão
P L
PL
Silveira
A M
AM
Gazzinelli
G
G
Prata
A
A
Coffman
R L
RL
Pizziolo
V
V
Souza
C P
CP
Colley
D G
DG
Correa-Oliveira
R
R
D000208:Acute Disease; D000818:Animals; D000911:Antibodies, Monoclonal / Q000494:pharmacology; D000947:Antigens, Helminth / Q000494:pharmacology; D002478:Cells, Cultured; D002908:Chronic Disease; D016207:Cytokines / Q000502:physiology*; D005260:Female; D006801:Humans; D016753:Interleukin-10 / Q000502:physiology; D015847:Interleukin-4 / Q000276:immunology / Q000502:physiology; D015848:Interleukin-5 / Q000276:immunology / Q000502:physiology; D007411:Intestinal Diseases, Parasitic / Q000276:immunology; D007963:Leukocytes, Mononuclear / Q000276:immunology*; D008109:Liver Diseases, Parasitic / Q000276:immunology; D008213:Lymphocyte Activation; D008297:Male; D051379:Mice; D012550:Schistosoma mansoni / Q000254:growth & development / Q000276:immunology*; D012555:Schistosomiasis mansoni / Q000276:immunology; D013158:Splenic Diseases / Q000276:immunology
D000911:Antibodies, Monoclonal; D000947:Antigens, Helminth; D016207:Cytokines; D015848:Interleukin-5; D016753:Interleukin-10; D015847:Interleukin-4