Modulation by lipopolysaccharide of inflammatory cytokine production by two T cell lines.
In this study, the modulation of inflammatory cytokine production by lipopolysaccharide (LPS) in two T cell lines, RL-male-1 and L5178Y-ML was examined. Both cell lines produced interleukin 1 (IL-1) activity in response to LPS, which was largely independent of the presence of serum. The IL-1 activity induced was neutralized by an anti-IL-1beta antibody, but not by an anti-IL-1alpha antibody. Induction of IL-1alpha and beta was also confirmed by polymerase chain reaction of reverse-transcribed mRNA (RT-PCR), although in RL-male-1 cells, IL-1alpha mRNA was constitutively expressed and somewhat enhanced by LPS. RT-PCR analysis revealed that these cell lines also upregulated tumour necrosis factor alpha (TNF-alpha) and IL-1 receptor antagonist mRNAs in response to LPS, although RL-male-1 cells expressed TNF-alpha mRNA constitutively. Flow cytometric analysis showed that, although these cells expressed Thy-1 antigen, they hardly expressed CD14 and gammadelta T cell receptor. In conclusion, LPS modulated inflammatory cytokine production in these T cell lines.
Uchimura
E
E
Laboratory of Molecular Immunology, Department of Biomolecular Science, Faculty of Science, Toho University, 2-2-1 Miyama, Funabashi, Chiba, 274, Japan.
Watanabe
N
N
Kobayashi
Y
Y
D000818:Animals; D016207:Cytokines / Q000096:biosynthesis*; D005434:Flow Cytometry; D007249:Inflammation / Q000276:immunology; D053590:Interleukin 1 Receptor Antagonist Protein; D007375:Interleukin-1 / Q000096:biosynthesis / Q000235:genetics; D008070:Lipopolysaccharides / Q000494:pharmacology*; D051379:Mice; D016133:Polymerase Chain Reaction; D011817:Rabbits; D017472:Receptors, Interleukin-1 / Q000037:antagonists & inhibitors; D012795:Sialoglycoproteins / Q000096:biosynthesis / Q000235:genetics; D013601:T-Lymphocytes / Q000187:drug effects / Q000276:immunology*; D014407:Tumor Cells, Cultured; D014409:Tumor Necrosis Factor-alpha / Q000096:biosynthesis / Q000235:genetics
D016207:Cytokines; C507764:Il1rn protein, mouse; D053590:Interleukin 1 Receptor Antagonist Protein; D007375:Interleukin-1; D008070:Lipopolysaccharides; D017472:Receptors, Interleukin-1; D012795:Sialoglycoproteins; D014409:Tumor Necrosis Factor-alpha