Multilamellar structures of DNA complexes with cationic liposomes.
Studies of DNA complexes with cationic liposomes are prompted by the search for nonviral DNA carriers for gene therapy. Recent experiments have identified a stable multilamellar phase in which ordered smectic layers of DNA alternate with cationic bilayers. In this paper we identify the forces governing DNA adsorption on cationic lamellae, including a membrane-induced attraction between the adsorbed DNA. Calculating the DNA interhelical spacing as a function of system composition, the model successfully explains recent surprising observations.
Dan
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Department of Chemical Engineering, University of Delaware, Newark 19716, USA. dan@che.udel.edu
D000327:Adsorption; D001665:Binding Sites; D055592:Biophysical Phenomena; D001703:Biophysics; D002412:Cations; D004247:DNA / Q000008:administration & dosage / Q000737:chemistry*; D004337:Drug Carriers; D015316:Genetic Therapy; D066298:In Vitro Techniques; D008081:Liposomes / Q000737:chemistry*; D046911:Macromolecular Substances; D008956:Models, Chemical; D055672:Static Electricity; D013499:Surface Properties; D013816:Thermodynamics
D002412:Cations; D004337:Drug Carriers; D008081:Liposomes; D046911:Macromolecular Substances; D004247:DNA