[Automation of the cytometric analysis of the DNA content of solid tumors].
An automatic machine, dedicated to solid tumor DNA ploidy quantitation has been built in order to provide pathologists with a tool usable in clinical practice. Main efforts were focused on an automation of each step of the analysis and on an elimination of any subjective choice, while preserving the quality of measurement. As the software is independent of the machine architecture, it offers performances which increase in parallel with the rapid evolution of the computers. An illustration of the various functionalities of the automaton is proposed through the study of deparaffined breast cancer samples.
Herlin
P
P
Laboratoire d'anatomie pathologique, Centre François-Baclesse, Caen, France.
Masson
E
E
Duigou
F
F
Plancoulaine
B
B
Signolle
J P
JP
Mandard
A M
AM
Angot
F
F
Deman
D
D
Belhomme
P
P
Joret
J B
JB
Datry
T
T
Rougereau
O
O
Bloyet
D
D
D001943:Breast Neoplasms / Q000737:chemistry / Q000473:pathology; D002467:Cell Nucleus / Q000737:chemistry / Q000473:pathology; D004273:DNA, Neoplasm / Q000032:analysis*; D005260:Female; D005434:Flow Cytometry; D006801:Humans; D007091:Image Processing, Computer-Assisted / Q000379:methods; D011003:Ploidies; D015203:Reproducibility of Results; D012984:Software
D004273:DNA, Neoplasm