Cloning of a gene encoding a putative xylanase with a cellulose-binding domain from Humicola grisea.
We have isolated a genomic clone of a putative xylanase gene (xyn1) from Humicola grisea by using the DNA fragment encoding a cellulose-binding domain of H. grisea cellobiohydrolase 1 as a probe. The translation product of the xyn1 gene predicts a xylanase of 429 amino acids in length, with a cellulose-binding domain in the C-terminus.
Iikura
H
H
Department of Biotechnology, Graduate School of Agricultural and Life Sciences, University of Tokyo, Japan.
Takashima
S
S
Nakamura
A
A
Masaki
H
H
Uozumi
T
T
D000595:Amino Acid Sequence; D001483:Base Sequence; D002482:Cellulose / Q000378:metabolism; D003001:Cloning, Molecular; D003062:Codon; D015342:DNA Probes; D004271:DNA, Fungal / Q000096:biosynthesis / Q000235:genetics; D005800:Genes, Fungal / Q000235:genetics*; D003904:Mitosporic Fungi / Q000378:metabolism*; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D043325:Xylan Endo-1,3-beta-Xylosidase; D014995:Xylosidases / Q000096:biosynthesis* / Q000235:genetics
D003062:Codon; D015342:DNA Probes; D004271:DNA, Fungal; D002482:Cellulose; D014995:Xylosidases; D043325:Xylan Endo-1,3-beta-Xylosidase