title: The effect of interleukin-1 beta and interleukin-4 on the expression of prostaglandin receptors EP1 and EP3 in amnion WISH cells.
abstract: OBJECTIVE
  Although prostaglandin E2 (PGE2) is believed to modulate biochemical and immunological events leading to parturition, the role of prostaglandin E receptors during labor has not been investigated.
  
  
  METHODS
  Amnion WISH cells were incubated in media containing increasing concentrations of either interleukin-1 beta (IL-1 beta) or IL-4. Increased EP1 and EP3 protein expression was determined by Western blot analysis with peptide-specific antibodies. Concomitant measurements of culture media PGE2 were made by an enzyme immunoassay.
  
  
  RESULTS
  Incubation of WISH cells with IL-1 beta or IL-4 caused a two- to three-fold increase in EP1 protein levels. IL-1 beta and IL-4 also caused six- and two-fold increases, respectively, in culture fluid PGE2 concentrations. IL-1 beta or IL-4 had no effect on EP3 protein levels.
  
  
  CONCLUSIONS
  Based on these results, it is proposed that IL-1 beta and IL-4 may be involved in the initiation and promotion of labor by inducing EP1 levels and PGE2 production in amnion.
authors.affiliation: Department of Obstetrics and Gynecology, University of South Florida, Tampa, FL 33612, USA.
authors.forename: E P
authors.initials: EP
authors.lastname: Spaziani
authors.forename: J C
authors.initials: JC
authors.lastname: Tsibris
authors.forename: L T
authors.initials: LT
authors.lastname: Hunt
authors.forename: R R
authors.initials: RR
authors.lastname: Benoit
authors.forename: W F
authors.initials: WF
authors.lastname: O'Brien
mesh_terms: D000650:Amnion / Q000187:drug effects / Q000276:immunology* / Q000378:metabolism*; D002460:Cell Line; D015232:Dinoprostone / Q000096:biosynthesis; D005260:Female; D006801:Humans; D007375:Interleukin-1 / Q000494:pharmacology*; D015847:Interleukin-4 / Q000494:pharmacology*; D007743:Labor, Obstetric / Q000276:immunology / Q000378:metabolism; D007752:Obstetric Labor, Premature / Q000209:etiology / Q000276:immunology / Q000378:metabolism; D011247:Pregnancy; D018078:Receptors, Prostaglandin E / Q000378:metabolism*; D058306:Receptors, Prostaglandin E, EP1 Subtype; D058308:Receptors, Prostaglandin E, EP3 Subtype
chemical_list: D007375:Interleukin-1; C545635:PTGER1 protein, human; C545647:PTGER3 protein, human; D018078:Receptors, Prostaglandin E; D058306:Receptors, Prostaglandin E, EP1 Subtype; D058308:Receptors, Prostaglandin E, EP3 Subtype; D015847:Interleukin-4; D015232:Dinoprostone