title: Anatomic distribution of melanocytes in normal nail unit: an immunohistochemical investigation.
abstract: Very few histologic reports describe normal melanocytes of the nail unit. Previous studies predominantly address the distal nail matrix melanocytes; we found no review of nail-bed melanocytes in the literature. The proximal nail matrix melanocytes are difficult to identify; the cells cannot be identified by L-DOPA staining. More recently, their scarcity was confirmed by immunohistochemistry with a large panel of antibodies directed against melanocytes. We wished to detect the proximal nail matrix dormant melanocytes and compare their density and distribution with that of the other melanocytes in the distal matrix and nail bed and to establish criteria of normality that may help clarify the pathologic features of benign nevoid melanonychia in the nails of whites. A panel of five monoclonal antibodies (MoAbs), including HMB45 and TRP1 directed against antigens localized in early melanosomal vesicles, was investigated in frozen sections of six nail specimens from whites. Both vertical and horizontal sections were assessed to determine the presence of dormant melanocytes. Results showed that the proximal nail matrix melanocytes were clearly identified with MoAbs HMB45 and tyrosinase-related protein-1 (TRP-1). By contrast, melanocytes stained by MoAb against tyrosinase and L-DOPA reaction were evident, especially in the distal matrix. With MoAb TRP-1, the epithelial sheets showed counts of approximately 217+/-84/mm2 in the proximal matrix and of 132+/-34/mm2 in the distal matrix; the nail bed counts were only 45+/-25/mm2. The split epithelial sheets had 103+/-17/mm2 L-DOPA-positive melanocytes in the distal third of the matrix, but only a few of them were detected in the proximal matrix and none were noted in the nail bed. We clearly identified proximal nail melanocytes using MoAb HMB45 and TRP1. The total number of matrix melanocytes can be estimated as approximately 217/mm2. In proximal matrix, the dormant melanocytes compartment was predominant. In the distal matrix, two compartments were identified: a functionally differentiated and a dormant compartment. Contrary to classical opinion, longitudinal melanonychia originates more frequently in the distal matrix, not secondary to the larger melanocyte density but because only the distal matrix contains an active melanin synthesis compartment. Furthermore, the superficial distribution of proximal nail melanocytes in vertical sections showed a histologic feature that may simulate the pagetoid pattern of melanoma in situ.
authors.affiliation: Department of Pathology, University of Nice, France.
authors.forename: C
authors.initials: C
authors.lastname: Perrin
authors.forename: J F
authors.initials: JF
authors.lastname: Michiels
authors.forename: A
authors.initials: A
authors.lastname: Pisani
authors.forename: J P
authors.initials: JP
authors.lastname: Ortonne
mesh_terms: D000368:Aged; D000911:Antibodies, Monoclonal; D000951:Antigens, Neoplasm; D000954:Antigens, Surface; D002452:Cell Count; D002454:Cell Differentiation; D004396:Coloring Agents; D015259:Dopamine Agents; D000078404:Epidermal Cells; D004847:Epithelial Cells / Q000166:cytology; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D007980:Levodopa; D008297:Male; D008543:Melanins / Q000096:biosynthesis; D008544:Melanocytes / Q000166:cytology* / Q000648:ultrastructure; D008545:Melanoma / Q000473:pathology; D058950:Melanoma-Specific Antigens; D008562:Membrane Glycoproteins; D008875:Middle Aged; D014442:Monophenol Monooxygenase; D009260:Nail Diseases / Q000473:pathology; D009262:Nails / Q000166:cytology*; D009363:Neoplasm Proteins; D010088:Oxidoreductases; D010859:Pigmentation Disorders / Q000473:pathology; D011506:Proteins; D014617:Vacuoles / Q000648:ultrastructure
chemical_list: D000911:Antibodies, Monoclonal; D000951:Antigens, Neoplasm; D000954:Antigens, Surface; D004396:Coloring Agents; D015259:Dopamine Agents; D008543:Melanins; D058950:Melanoma-Specific Antigens; D008562:Membrane Glycoproteins; D009363:Neoplasm Proteins; D011506:Proteins; D007980:Levodopa; D010088:Oxidoreductases; C489799:TYRP1 protein, human; C064056:tyrosinase-related protein-1; D014442:Monophenol Monooxygenase