Inactivation of mdoH leads to increased expression of colanic acid capsular polysaccharide in Escherichia coli.
Capsule gene (cps) expression, which normally occurs at low levels in Escherichia coli lon+ cells, increased 38-fold in lon+ cells carrying a Tn10::delta kan insertion mapping to 24 min on the E. coli chromosome. Null mutations in rcsA, rcsB, or rcsC abolished the effect of the Tn10::delta kan insertion. Sequencing of both sides of the Tn10::delta kan insertion localized the insertion to the previously reported mdoH gene, which encodes a protein involved in biosynthesis of membrane-derived oligosaccharides (MDOs). A model suggesting that the periplasmic levels of MDOs act to signal RcsC to activate cps expression is proposed.
Ebel
W
W
Department of Microbiology, Oregon State University, Corvallis 97331-3804, USA.
Vaughn
G J
GJ
Peters
H K
HK
Trempy
J E
JE
D049069:ATP-Dependent Proteases; D016667:Bacterial Capsules / Q000096:biosynthesis*; D001426:Bacterial Proteins / Q000235:genetics*; D004926:Escherichia coli / Q000235:genetics*; D029968:Escherichia coli Proteins; D015964:Gene Expression Regulation, Bacterial; D006360:Heat-Shock Proteins / Q000235:genetics; D008565:Membrane Proteins / Q000235:genetics*; D009097:Multienzyme Complexes; D016254:Mutagenesis, Insertional; D009997:Osmotic Pressure; D010749:Phosphoprotein Phosphatases; D011134:Polysaccharides / Q000096:biosynthesis*; D049070:Protease La; D011494:Protein Kinases; D012697:Serine Endopeptidases / Q000235:genetics; D014157:Transcription Factors / Q000235:genetics
D001426:Bacterial Proteins; D029968:Escherichia coli Proteins; D006360:Heat-Shock Proteins; D008565:Membrane Proteins; D009097:Multienzyme Complexes; D011134:Polysaccharides; C489568:RcsB protein, E coli; D014157:Transcription Factors; C089831:opgH protein, E coli; C489728:rcsC protein, E coli; C074889:RcsB protein, Bacteria; C067572:RcsA protein, E coli; C004275:colanic acid; D011494:Protein Kinases; D010749:Phosphoprotein Phosphatases; D049069:ATP-Dependent Proteases; D012697:Serine Endopeptidases; C483567:Lon protein, E coli; D049070:Protease La