Use of hematopoietic cytokines to accelerate the recovery of the immune system in irradiated mice.
In our previous studies aimed at designing appropriate strategies to accelerate recovery of the immune system after irradiation, we found that the hematopoietic cytokine recombinant murine (rmu) interleukin (IL)-3 was able to induce differentiation and growth of thymocytes and splenic T and B lymphocytes in mice exposed to x-rays (200-500 cGy). The recovery, however, was complete at 7 days only after a dose of 200 cGy, whereas 2, 3, and 4 weeks were necessary to achieve full recovery after 300, 400, and 500 cGy, respectively. These studies were extended to investigate the effects of another hematopoietic cytokine, recombinant human (rhu) IL-11, a bone marrow stromal-derived cytokine, administered together with IL-3 to irradiated mice. The synergistic effect of the two cytokines was evident when relatively small doses of rhu IL-11 were injected with an optimal dose of rmu IL-3.
Frasca
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Laboratory of Immunology, AMB-PRO-TOSS, ENEA CR Casaccia, Rome, Italy.
Guidi
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Arbitrio
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Pioli
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Leter
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Spano
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Doria
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D000818:Animals; D001402:B-Lymphocytes / Q000187:drug effects / Q000276:immunology / Q000528:radiation effects*; D001854:Bone Marrow Cells / Q000187:drug effects / Q000528:radiation effects; D015496:CD4-Positive T-Lymphocytes / Q000528:radiation effects; D018414:CD8-Positive T-Lymphocytes / Q000528:radiation effects; D002453:Cell Cycle / Q000187:drug effects / Q000528:radiation effects; D016207:Cytokines / Q000494:pharmacology; D004307:Dose-Response Relationship, Radiation; D006410:Hematopoiesis / Q000528:radiation effects; D006801:Humans; D017370:Interleukin-11 / Q000494:pharmacology*; D007377:Interleukin-3 / Q000494:pharmacology*; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D008940:Mitotic Index / Q000187:drug effects / Q000528:radiation effects; D011994:Recombinant Proteins / Q000494:pharmacology; D013154:Spleen / Q000187:drug effects / Q000276:immunology / Q000528:radiation effects; D013601:T-Lymphocytes / Q000187:drug effects / Q000276:immunology / Q000528:radiation effects*; D013950:Thymus Gland / Q000187:drug effects / Q000276:immunology / Q000528:radiation effects; D014916:Whole-Body Irradiation; D014965:X-Rays
D016207:Cytokines; D017370:Interleukin-11; D007377:Interleukin-3; D011994:Recombinant Proteins