Characterization of the gene (VBP1) and transcript for the von Hippel-Lindau binding protein and isolation of the highly conserved murine homologue.
A single-copy, widely expressed gene of at least 30 kb and six exons was discovered via a hybrid mRNA resulting from an inversion causing hemophilia A. A segment of the 1.7-kb message of this gene has been shown by others to encode a protein (named VBP1) interacting with the product of the von Hippel-Lindau gene and thus is expected to participate in pathways involving this tumor suppressor gene. The mouse VBP1 message we isolated encodes a polypeptide of 160 residues absolutely identical to that of human. Even the 3' untranslated tails of the mRNAs show 68% conservation, and both use the unusual ATTAAA polyadenylation signal. The mouse gene has a single transcription start while the human homologue has two major starts and a minor start. This could result in amino-end extensions of the human protein. A polymorphism with 42% heterozygosity in the United Kingdom population was detected in the 3' tail of the message. VBP1 is unlike other known proteins but a consensus for tyrosine phosphorylation possibly suggests regulation by kinases.
Brinke
A
A
Division of Medical & Molecular Genetics, United Medical School, Guy's Hospital, London, United Kingdom.
Green
P M
PM
Giannelli
F
F
D000818:Animals; D001483:Base Sequence; D002352:Carrier Proteins / Q000235:genetics*; D017124:Conserved Sequence; D003598:Cytoskeletal Proteins; D018076:DNA, Complementary; D006801:Humans; D051379:Mice; D018832:Molecular Chaperones; D008969:Molecular Sequence Data; D011110:Polymorphism, Genetic; D011401:Promoter Regions, Genetic; D014176:Protein Biosynthesis; D012333:RNA, Messenger / Q000235:genetics*; D014158:Transcription, Genetic
D002352:Carrier Proteins; D003598:Cytoskeletal Proteins; D018076:DNA, Complementary; D018832:Molecular Chaperones; D012333:RNA, Messenger; C108825:VBP1 protein, human; C484640:Vbp1 protein, mouse