title: Ca2+ and Ba2+ effects on basolateral tetraethylammonium transport in isolated snake renal proximal tubules.
abstract: Previous work on snake renal tubules suggested that basolateral transport of tetraethylammonium (TEA) is symmetrical. To examine regulation of this transport step more closely, we determined the effects of (1) reductions in the extracellular Ca2+ concentration and basolateral Ca2+ entry, and (2) the presence of extracellular Ba2+ on TEA uptake and efflux across the basolateral membrane of isolated snake renal proximal tubules. Removal of extracellular Ca2+ reduced initial TEA uptake and enhanced TEA efflux. Blocking Ca2+ entry also reduced initial TEA uptake. Extracellular Ba2+ depolarized the basolateral membrane and reduced both TEA uptake and efflux. Inhibition of basolateral TEA uptake with a reduced membrane potential supports previous data indicating that uptake involves potential-driven facilitated diffusion. Inhibition of basolateral TEA efflux by Ba2+ even with a reduced membrane potential not only supports previously obtained data indicating that efflux is not influenced by the potential difference and that basolateral TEA transport is asymmetrical, but also suggests that TEA uptake and efflux may occur by separate pathways.
authors.affiliation: Department of Physiology, College of Medicine, University of Arizona, Tucson, AZ 85724-5051, USA.
authors.forename: Y K
authors.initials: YK
authors.lastname: Kim
authors.forename: W H
authors.initials: WH
authors.lastname: Dantzler
mesh_terms: D000818:Animals; D001464:Barium / Q000494:pharmacology*; D001692:Biological Transport / Q000187:drug effects; D002118:Calcium / Q000008:administration & dosage / Q000494:pharmacology*; D017812:Colubridae; D004533:Egtazic Acid / Q000494:pharmacology; D007687:Kidney Tubules, Proximal / Q000187:drug effects* / Q000378:metabolism*; D007811:Lanthanum / Q000494:pharmacology; D008564:Membrane Potentials; D019789:Tetraethylammonium / Q000378:metabolism*; D014316:Tritium; D014700:Verapamil / Q000494:pharmacology
chemical_list: D014316:Tritium; D001464:Barium; D004533:Egtazic Acid; D019789:Tetraethylammonium; D007811:Lanthanum; D014700:Verapamil; D002118:Calcium