The pkI gene encoding pyruvate kinase I links to the luxZ gene which enhances bioluminescence of the lux operon from Photobacterium leiognathi.
Partial 3'-end nucleotide sequence of the pkI gene (GenBank accession No. AF019143) from Photobacterium leiognathi ATCC 25521 has been determined, and the encoded pyruvate kinase I is deduced. Pyruvate kinase I is the key enzyme of glycolysis, which converts phosphoenol pyruvate to pyruvate. Alignment and comparison of pyruvate kinase Is from P. leiognathi, E. coli and Salmonella typhimurium show that they are homologous. Nucleotide sequence reveals that the pkI gene is linked to the luxZ gene that enhances bioluminescence of the lux operon from P. leiognathi. The gene order of the pkI and luxZ genes is-pk1-ter-->-R&R"-luxZ-ter"-->, whereas ter is transcriptional terminator for the pkI and related genes, and R&R" is the regulatory region and ter" is transcriptional terminator for the luxZ gene. It clearly elicits that the pkI gene and luxZ gene are divided to two operons. Functional analysis confirms that the potential hairpin loop omega T is the transcriptional terminator for the pkI and related genes. It infers that the pkI and related genes are simply linked to the luxZ gene in P. leiognathi genome.
Lin
J W
JW
Institute of Molecular Biology, National Chung Hsing University, Taichung, Taiwan, Republic of China. jwlin@dragon.nchu.edu.tw
Lu
H C
HC
Chen
H Y
HY
Weng
S F
SF
D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000378:metabolism*; D001483:Base Sequence; D008163:Luminescent Measurements; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation; D009876:Operon; D010776:Photobacterium / Q000201:enzymology* / Q000235:genetics; D011770:Pyruvate Kinase / Q000235:genetics*; D015183:Restriction Mapping; D016415:Sequence Alignment; D013045:Species Specificity; D014158:Transcription, Genetic
D001426:Bacterial Proteins; D011770:Pyruvate Kinase