Morphological alterations of blood platelets induced by platelet activating factor (PAF) and partial inhibition by ketoprofen in calves.
The influence of platelet activating factor (PAF) was investigated in vivo on the ultrastructure of bovine platelets, and on the platelet count. The effect of an intravenous administration of ketoprofen (a non-steroidal anti-inflammatory drug) pretreatment followed by PAF infusion was also observed in a group of six healthy male Friesian calves. PAF infusion alone caused a moderate thrombocytopenia, which peaked one minute post challenge and returned to levels not significantly different from control after 30 min. Electron microscopy revealed that after PAF infusion, platelets lost their lentiform shape and became irregular, with many pseudopods. Their microtubules became impossible to distinguish. The numbers of alpha granules and dense bodies were significantly decreased. Glycogen particles became rare or even disappeared. Giant platelets occasionally appeared. The Golgi apparatus was more often visible and the number of mitochondria was significantly increased. Ketoprofen pretreatment lowered PAF-induced thrombocytopenia and decrease in the number of dense bodies. Under these conditions, the Golgi apparatus was rarely visible and giant platelets were not observed. These results showed that the morphological ultrastructure of blood platelets in bovines were modified following PAF infusion and that ketoprofen pretreatment before PAF infusion provided partial protection, limiting the extent of the morphological alterations and maintaining a normal platelet count.
Bastos da Silva
M
M
Laboratoire d'investigations fonctionnelles, service de physiologie, Liège, Belgium. physio@stat.fmv.ulg.ac.be
Dessy
C
C
David
J L
JL
Lekeux
P
P
D000818:Animals; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000008:administration & dosage / Q000494:pharmacology*; D001792:Blood Platelets / Q000187:drug effects* / Q000648:ultrastructure; D002417:Cattle; D003594:Cytoplasmic Granules / Q000187:drug effects / Q000648:ultrastructure; D007262:Infusions, Intravenous; D007660:Ketoprofen / Q000008:administration & dosage / Q000494:pharmacology*; D008297:Male; D010972:Platelet Activating Factor / Q000008:administration & dosage / Q000037:antagonists & inhibitors / Q000494:pharmacology*; D010976:Platelet Count / Q000187:drug effects*; D013921:Thrombocytopenia / Q000139:chemically induced; D013997:Time Factors
D000894:Anti-Inflammatory Agents, Non-Steroidal; D010972:Platelet Activating Factor; D007660:Ketoprofen