[Use of flow cytofluorimetry in the analysis of interactions of mononuclear phagocytes with protein antigens].
Flow cytofluorometry of samples stained with fluorochrome-labeled antigen may be used to study the interactions between human blood calls and antigens. Using fluorescein isothiocyanate-labeled tuberculin (after the original method), the authors found that human blood monocytes actively bind labeled tuberculin. Studies of the concentrations from 0.1 to 12,500 ng/ml showed that saturation of the cell capacity for endocytosis is attained at certain doses, after which their surface label prevails. The share of tuberculin-binding lymphocytes was appreciably lower than that of monocytes at the same doses of the antigen. Clinical application of the method for assessing the antigen-presenting function of macrophages is discussed.
Stenina
M A
MA
Kavokin
A A
AA
Nikonenko
B N
BN
Simonova
A V
AV
D000941:Antigens / Q000378:metabolism*; D004705:Endocytosis; D005434:Flow Cytometry; D005456:Fluorescent Dyes; D006801:Humans; D008214:Lymphocytes / Q000378:metabolism; D009000:Monocytes / Q000378:metabolism*; D011485:Protein Binding; D014373:Tuberculin / Q000378:metabolism*
D000941:Antigens; D005456:Fluorescent Dyes; D014373:Tuberculin