Vegetative tissue lectins of peanut (A. hypogaea).
Lectin activities in roots, nodules, stems and leaves of 1-6 week old peanut plant (A. hypogaea) were checked by erythrocyte (human and rabbit) agglutination and sugar inhibition assays. Human and rabbit erythrocyte agglutinating activities were specifically inhibited by lactose/cellobiose (SLII) and methyl alpha-mannoside (SLI) respectively. Seeds, embryos and cotyledons agglutinated neuraminidase treated human erythrocytes and that activity was inhibited by T-disaccharide. In the roots of field grown plants SLI was the major activity, while nodules showed both activities (SLI and SLII). Specific activities of SLI and SLII were maximal in stem tissue and hypocotyl exhibited minimal levels. Actively growing tissues like newly emerging young leaves and elongating stem contained more SLII activity in comparison to the mature tissues. Immunological test indicated that all the vegetative tissue lectins are serologically related.
Singh
R
R
Centre for Biochemical Technology, Delhi, India.
Gautam
H
H
Jayaraman
V
V
Nair
G
G
Das
H
H
D000818:Animals; D010367:Arachis / Q000254:growth & development / Q000378:metabolism; D006386:Hemagglutination Tests; D006801:Humans; D066298:In Vitro Techniques; D019887:Peanut Agglutinin / Q000378:metabolism*; D037121:Plant Lectins; D011817:Rabbits; D012639:Seeds / Q000378:metabolism; D014018:Tissue Distribution
D019887:Peanut Agglutinin; D037121:Plant Lectins