title: Detection of DNA lesions induced by chemical mutagens by the single cell gel electrophoresis (Comet) assay. 1. Relationship between the onset of DNA damage and the characteristics of mutagens.
abstract: We evaluated the relationship between the onset of DNA damage and the characteristics of 5 model chemical mutagens with the single-cell gel electrophoresis (SCG) assay using L5178Y mouse lymphoma cells. We treated the cells with each chemical for 3 h and sampled them 0, 21, and 45 h after treatment. DNA damage induced by UV mimetic mutagens MMS and MNU, and X-ray mimetic mutagen BLM was observed just after treatment, crosslinking agent MMC-induced DNA damage was detected 21 h after treatment, and 6-MP as an inhibitor of DNA synthesis did not induce DNA damage at any sampling time. These results suggest that the SCG assay detects DNA lesions just after treatment with UV and X-ray mimetic mutagens, but needs a waiting period after treatment with crosslinking agents.
authors.affiliation: Toxicology Research Laboratories, Fujisawa Pharmaceutical Co., Ltd., Osaka, Japan.
authors.forename: Y
authors.initials: Y
authors.lastname: Miyamae
authors.forename: K
authors.initials: K
authors.lastname: Zaizen
authors.forename: K
authors.initials: K
authors.lastname: Ohara
authors.forename: Y
authors.initials: Y
authors.lastname: Mine
authors.forename: Y F
authors.initials: YF
authors.lastname: Sasaki
mesh_terms: D000818:Animals; D001761:Bleomycin / Q000633:toxicity; D003432:Cross-Linking Reagents / Q000633:toxicity; D004249:DNA Damage; D004586:Electrophoresis / Q000379:methods; D007940:Leukemia L5178; D015122:Mercaptopurine / Q000633:toxicity; D008741:Methyl Methanesulfonate / Q000633:toxicity; D008770:Methylnitrosourea / Q000633:toxicity; D051379:Mice; D016685:Mitomycin / Q000633:toxicity; D009152:Mutagenicity Tests / Q000379:methods*; D009153:Mutagens / Q000633:toxicity*; D019384:Nucleic Acid Synthesis Inhibitors / Q000633:toxicity; D013997:Time Factors; D014407:Tumor Cells, Cultured; D014466:Ultraviolet Rays; D014965:X-Rays
chemical_list: D003432:Cross-Linking Reagents; D009153:Mutagens; D019384:Nucleic Acid Synthesis Inhibitors; D001761:Bleomycin; D016685:Mitomycin; D008770:Methylnitrosourea; D008741:Methyl Methanesulfonate; D015122:Mercaptopurine