title: Erythrocyte, platelet and polymorphonuclear leukocyte membrane dynamic properties in essential hypertension.
abstract: In subjects with essential hypertension we evaluated, respectively, the red cell membrane protein lateral mobility (obtained marking intact red blood cells with pyrene-3-maleimide (3-PM)), the erythrocyte membrane fluidity (obtained marking intact erythrocytes with 10-(1-pyrene) decanoic acid), the red cell membrane transverse fluidity gradient (obtained marking intact red blood cells with a set of fatty acid fluorescent probes (2-AP, 6-AS, 9-AS, 12-AS)), the platelet membrane fluidity (obtained marking intact and unstimulated platelets with 1,6-diphenyl-1,3,5-hexatriene (DPH) and with 1-(4-(trimethylamino)phenyl)-6-phenyl-1,3,5-hexatriene (TMA-DPH)) and the polymorphonuclear membrane fluidity (obtained marking intact and unstimulated polymorphonuclear cells with TMA-DPH). From the obtained data it is evident that: (1) red cell membrane protein lateral mobility does not distinguish normals from hypertensives; (2) erythrocyte membrane fluidity and red cell membrane transverse fluidity gradient clearly discriminate normals from hypertensives; (3) platelet membrane fluidity differentiates normals from hypertensives only when DPH is used as fluorescent probe; (4) polymorphonuclear membrane fluidity does not distinguish normals from hypertensives. Our results show that in essential hypertension a different behaviour of the membrane dynamic properties in the circulating blood cells is evident.
authors.affiliation: Istituto di Clinica Medica e Malattie Cardiovascolari, Università degli Studi di Palermo, Italy.
authors.forename: G
authors.initials: G
authors.lastname: Caimi
mesh_terms: D000328:Adult; D001792:Blood Platelets / Q000502:physiology*; D004910:Erythrocyte Membrane / Q000502:physiology*; D005260:Female; D005454:Fluorescence Polarization; D006801:Humans; D006973:Hypertension / Q000097:blood*; D008297:Male; D008560:Membrane Fluidity / Q000502:physiology*; D008875:Middle Aged; D009504:Neutrophils / Q000502:physiology*