Effects of hydrostatic pressure on the activity of rat ribosome and cell-free translation system.
The effects of high hydrostatic pressure on the protein synthesis activity of rat liver ribosome and the reconstructed cell-free translation system were studied. The results indicated that the activity of the ribosome decreased with the increase in applied pressure and the activity was totally lost as pressure went up to 2,400 bar, but there was a plateau approximately from 300 to 1,200 bar where the activity only had a minor change. The activity of the cell-free translation system seems to be more sensitive to pressure. Its activity was entirely lost when the pressure was up to 900 bar. The activity of rat ribosome and the cell-free translation system treated by pressure below 900 bar could be almost completely restored by incubation after releasing the pressure, but could be partially restored at higher pressure.
Lu
B
B
Shanghai Institute of Biochemistry, Academia Sinica, Shanghai, China.
Li
Q
Q
Liu
W Y
WY
Ruan
K C
KC
D000818:Animals; D002474:Cell-Free System / Q000378:metabolism; D006874:Hydrostatic Pressure; D008099:Liver / Q000378:metabolism*; D011506:Proteins / Q000138:chemical synthesis*; D051381:Rats; D017207:Rats, Sprague-Dawley; D012270:Ribosomes / Q000378:metabolism*
D011506:Proteins