Isolation of nuclei for chromatin analysis in fission yeast.
The methods available for analysis of the chromatin of Schizosaccharomyces pombe are time consuming (>8 h) and/or result in some degradation of the chromatin. Here we report an optimised method for the preparation of spheroplasts and the isolation of nuclei which takes <25 min and is suitable for analysis of chromatin structure by micrococcal nuclease, restriction endonuclease or by immunoprecipitation.
Mason
J A
JA
Department of Biochemistry, University of Oxford, South Parks Road, Oxford OX1 3QU, UK.
Mellor
J
J
D002458:Cell Fractionation / Q000379:methods*; D002467:Cell Nucleus; D002843:Chromatin; D012568:Schizosaccharomyces / Q000166:cytology* / Q000235:genetics
D002843:Chromatin