Glibenclamide sensitivity of neural relaxation of the rabbit sphincter of oddi.
In this article we studied whether the nitrergic relaxation of the rabbit sphincter was sensitive to glibenclamide. Field stimulation relaxed the sphincter of Oddi rings after incubation with atropine (1 microM) and guanethidine (4 microM) with threefold and fourfold increases in tissue guanosine 3':5'-cyclic monophosphate and adenosine 3':5'-cyclic monophosphate contents, respectively. These changes were blocked by 30 microM NG-nitro-L-arginine methyl ester. Glibenclamide (0.1-10 microM) attenuated the field stimulation-induced relaxation and completely abolished the relaxation produced by 0.1 microM cromakalim. We conclude that nitrergic relaxation of the rabbit sphincter of Oddi comprises a mechanism sensitive to glibenclamide.
Szilvassy
Z
Z
First Department of Medicine, Albert Szent-Györgyi Medical University, Szeged, 6701, Hungary.
Lonovics
J
J
Jakab
I
I
Nagy
I
I
D000818:Animals; D000242:Cyclic AMP / Q000378:metabolism; D006152:Cyclic GMP / Q000378:metabolism; D005905:Glyburide / Q000494:pharmacology*; D066298:In Vitro Techniques; D008297:Male; D009126:Muscle Relaxation; D009130:Muscle, Smooth / Q000187:drug effects* / Q000294:innervation / Q000378:metabolism; D019331:NG-Nitroarginine Methyl Ester / Q000494:pharmacology; D026902:Potassium Channel Blockers; D011817:Rabbits; D009803:Sphincter of Oddi / Q000187:drug effects* / Q000294:innervation / Q000378:metabolism
D026902:Potassium Channel Blockers; D000242:Cyclic AMP; D006152:Cyclic GMP; D005905:Glyburide; D019331:NG-Nitroarginine Methyl Ester