Localization of B-DNA and Z-DNA in terminally differentiating fiber cells in the adult lens.
We examined histochemically and immunohistochemically the distribution of B- and Z-DNA in the epithelium and terminally differentiating dog lens fiber cells. On the basis of anti-DNA antibody reactivity, qualitative and quantitative data on B- and Z-DNA in cells were determined. Anti-B-DNA immunoreactivity gradually declined throughout nucleated fibers, with a precipitous decrease at approximately 90 microns. Anti-Z-DNA antibody binding decreased with a sudden loss of immunoreactivity at approximately 90 microns. The pattern of anti-B- and Z-DNA staining correlates with the loss of alpha-crystallin immunoreactivity, the major lens crystallin, and decreased eosin staining of proteins. Germinative zone cell nuclei showed the highest DNA probe binding values, followed by the superficial fibers, central zone, middle fibers, and deep fibers. The presence of single-stranded (ss)DNA in deeper fibers was detected by anti-ss-DNA antibodies. This is indicative of DNA degradation. These observations suggest that a dramatic reorganization of lens fiber cells' supramolecular order occurs at approximately 90 microns, the phase transition zone.
Gagna
C E
CE
Department of Ophthalmology, University of Medicine and Dentistry of New Jersey-Medical School, Newark 07103, USA.
Lambert
W C
WC
Kuo
H R
HR
Farnsworth
P N
PN
D000328:Adult; D000818:Animals; D003459:Crystallins / Q000032:analysis; D004247:DNA / Q000032:analysis*; D004277:DNA, Single-Stranded / Q000032:analysis; D004285:Dogs; D004801:Eosine Yellowish-(YS); D004848:Epithelium / Q000737:chemistry; D005456:Fluorescent Dyes; D006651:Histocytochemistry; D006801:Humans; D007091:Image Processing, Computer-Assisted; D007150:Immunohistochemistry; D007908:Lens, Crystalline / Q000737:chemistry*
D003459:Crystallins; D004277:DNA, Single-Stranded; D005456:Fluorescent Dyes; D004247:DNA; D004801:Eosine Yellowish-(YS)