title: HLA-A typing: comparison between serology, the amplification refractory mutation system with polymerase chain reaction and sequencing.
abstract: In this study we typed HLA-A polymorphisms by a new sequence-based typing (SBT) method, which involved one PCR reaction and four sequencing reactions covering exon 2 and exon 3. This method allowed complete identification of all known HLA-A alleles and revealed the presence of a new allele, named HLA-A*2608. We also introduced sequencing of exon 4 for some samples in order to discriminate the allelic pairs that are identical in exon 2 and 3, thus improving SBT resolution. Finally, we compared the results obtained by SBT with data obtained by serological typing and the amplification refractory mutation system (ARMS-PCR). Together, our results suggest that the SBT here described provides an optimal HLA-A typing technique that may be useful in selecting donor-recipient pairs in bone marrow transplantation between unrelated individuals.
authors.affiliation: National Institute for Cancer Research, IST, Advanced Biotechnology Center, Genova, Italy.
authors.forename: C
authors.initials: C
authors.lastname: Pera
authors.forename: L
authors.initials: L
authors.lastname: Delfino
authors.forename: A
authors.initials: A
authors.lastname: Morabito
authors.forename: A
authors.initials: A
authors.lastname: Longo
authors.forename: L
authors.initials: L
authors.lastname: Johnston-Dow
authors.forename: C B
authors.initials: CB
authors.lastname: White
authors.forename: M
authors.initials: M
authors.lastname: Colonna
authors.forename: G B
authors.initials: GB
authors.lastname: Ferrara
mesh_terms: D000328:Adult; D000483:Alleles; D001483:Base Sequence; D001782:Blood Donors; D016026:Bone Marrow Transplantation / Q000276:immunology; D015343:DNA Probes, HLA; D005069:Evaluation Studies as Topic; D005091:Exons / Q000235:genetics; D005805:Genes, MHC Class I; D015234:HLA-A Antigens / Q000032:analysis* / Q000235:genetics; D006650:Histocompatibility Testing / Q000379:methods*; D006801:Humans; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D011933:Reagent Kits, Diagnostic; D012680:Sensitivity and Specificity; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D012689:Sequence Homology, Nucleic Acid; D012698:Serologic Tests; D014019:Tissue Donors
chemical_list: D015343:DNA Probes, HLA; D015234:HLA-A Antigens; D011933:Reagent Kits, Diagnostic