[Detection of DNA single-copy sequences of prehistoric teeth. Site milieu as a factor for preservation of DNA].
DNA-extracts from prehistoric roots of teeth were analyzed by PCR to investigate microsatellite-systems. At the same time the x/y-chromosome specific system Amel A/B was investigated. The samples were collected from different burial conditions of similar age. Factors leading to limited DNA-degradation are given. For the first time reproducible Quadruplex-amplification on single-copy loci of prehistoric tissue was obtained in samples from the Lichtenstein-Cave, Kr. Osterode/Harz. Positive results are explained by low temperature in the burial site. In addition the results show that desiccation allows DNA-preservation but cannot protect the DNA from damage due to microbial origin. Microorganisms can destroy DNA-structures completely.
Burger
J
J
Institut für Anthropologie, Universität Göttingen.
Hummel
S
S
Herrmann
B
B
D002048:Burial / Q000266:history*; D004247:DNA / Q000266:history*; D049690:History, Ancient; D006801:Humans; D018895:Microsatellite Repeats; D010161:Paleodontology; D016133:Polymerase Chain Reaction; D014092:Tooth Root / Q000473:pathology*
D004247:DNA