In vitro systems for the reconstitution of snRNP and protein nuclear import.
In this chapter we have presented the most recent methods for the preparation of cell extracts and recombinant protein factors for the reconstitution of nuclear protein and snRNP import in vitro. In addition, we have discussed methods available for the quantitation of the level of import into nuclei. Accurate quantitation is particularly important when the effects of inhibitors are to be compared and when estimates of nuclear import rate are required.
Dingwall
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C
Department of Pharmacology, State University of New York at Stony Brook 11794-8651, USA.
Palacios
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I
D000818:Animals; D001692:Biological Transport; D002457:Cell Extracts; D002467:Cell Nucleus / Q000378:metabolism*; D003584:Cytological Techniques; D006801:Humans; D007091:Image Processing, Computer-Assisted; D011994:Recombinant Proteins / Q000378:metabolism; D017411:Ribonucleoproteins, Small Nuclear / Q000378:metabolism*
D002457:Cell Extracts; D011994:Recombinant Proteins; D017411:Ribonucleoproteins, Small Nuclear