Prostaglandin F2alpha-induced nest-building in pseudopregnant pigs. II. Space restriction stress does not influence secretion of oxytocin, prolactin, oestradiol or progesterone.
We have previously shown that prostaglandin F2alpha (PG) is capable of inducing nest-building behaviour in pseudopregnant gilts and established a protocol. This experiment examined which reproductive endocrine systems might mediate these behavioural responses, in the presence or absence of a space restriction stress. Pseudopregnancy was induced with 5 mg/day i.m. (intramuscular) injections of oestradiol valerate (OV) on Days 11-15 of the oestrous cycle, jugular vein catheters were placed on Day 39 of pseudopregnancy, and blood samples were collected daily from Day 40 to Day 48. On Day 42, gilts were either space restricted to farrowing crates 1.6 x 0.6 m (C: n = 11) or left in pens 2.8 x 1.74 m (P: n = 11). On Day 47, blood samples were collected from all animals every 15 min from 90 min prior to a single i.m. injection of 15 mg of prostaglandin F2alpha (PG: Lutalyse, Upjohn, Crowley, West Sussex) to 120 min post-PG and then hourly for 4 h and assayed for oxytocin, prolactin, progesterone, and oestradiol. Results showed that mean daily concentrations of prolactin and progesterone were significantly lower (p < 0.05 respectively) in C than P gilts from Day 42 to Day 46 of pseudopregnancy. There were no significant differences in mean daily concentrations of oxytocin and oestradiol between C and P gilts during this time. For both groups, oxytocin, prolactin, and progesterone concentrations increased significantly (p < 0.05) post-PG when compared to their respective pre-PG values. However, for both groups, oestradiol concentrations were unaffected by PG injection. The prostaglandin-induced increases in oxytocin, prolactin, and progesterone concentrations did not differ between groups. We conclude that coincident changes in oestradiol secretion does not influence nesting behaviour and that space restriction stress associated with nest-building does not influence secretion of oxytocin, prolactin, oestradiol, or progesterone.
Boulton
M I
MI
Department of Neurobiology, The Babraham Institute, Cambridge, United Kingdom. Margaret-I.Boulton@BBSRC.AC.UK
Wickens
A
A
Brown
D
D
Goode
J A
JA
Gilbert
C L
CL
D000818:Animals; D001143:Arousal / Q000187:drug effects* / Q000502:physiology; D015237:Dinoprost / Q000494:pharmacology*; D004958:Estradiol / Q000097:blood*; D005260:Female; D006799:Housing, Animal; D009425:Nesting Behavior / Q000187:drug effects* / Q000502:physiology; D010120:Oxytocics / Q000494:pharmacology*; D010121:Oxytocin / Q000097:blood*; D011374:Progesterone / Q000097:blood*; D011388:Prolactin / Q000097:blood*; D011555:Pseudopregnancy / Q000503:physiopathology*; D012016:Reference Values; D012636:Secretory Rate / Q000187:drug effects; D012931:Social Environment; D013552:Swine
D010120:Oxytocics; D011374:Progesterone; D004958:Estradiol; D010121:Oxytocin; D011388:Prolactin; D015237:Dinoprost