title: Synaptic clustering of the cell adhesion molecule fasciclin II by discs-large and its role in the regulation of presynaptic structure.
abstract: The cell adhesion molecule Fasciclin II (FASII) is involved in synapse development and plasticity. Here we provide genetic and biochemical evidence that proper localization of FASII at type I glutamatergic synapses of the Drosophila neuromuscular junction is mediated by binding between the intracellular tSXV bearing C-terminal tail of FASII and the PDZ1-2 domains of Discs-Large (DLG). Moreover, mutations in fasII and/or dlg have similar effects on presynaptic ultrastructure, suggesting their functional involvement in a common developmental pathway. DLG can directly mediate a biochemical complex and a macroscopic cluster of FASII and Shaker K+ channels in heterologous cells. These results indicate a central role for DLG in the structural organization and downstream signaling mechanisms of cell adhesion molecules and ion channels at synapses.
authors.affiliation: Department of Neurochemistry and Molecular Biology, Federal Institute for Neurobiology, Magdeburg, Germany.
authors.forename: U
authors.initials: U
authors.lastname: Thomas
authors.forename: E
authors.initials: E
authors.lastname: Kim
authors.forename: S
authors.initials: S
authors.lastname: Kuhlendahl
authors.forename: Y H
authors.initials: YH
authors.lastname: Koh
authors.forename: E D
authors.initials: ED
authors.lastname: Gundelfinger
authors.forename: M
authors.initials: M
authors.lastname: Sheng
authors.forename: C C
authors.initials: CC
authors.lastname: Garner
authors.forename: V
authors.initials: V
authors.lastname: Budnik
mesh_terms: D000818:Animals; D019556:COS Cells; D015816:Cell Adhesion Molecules, Neuronal / Q000032:analysis / Q000096:biosynthesis*; D003001:Cloning, Molecular; D004330:Drosophila / Q000235:genetics; D029721:Drosophila Proteins; D016147:Genes, Tumor Suppressor; D007150:Immunohistochemistry; D019476:Insect Proteins / Q000096:biosynthesis; D008322:Mammals; D016253:Microscopy, Immunoelectron; D016296:Mutagenesis; D009469:Neuromuscular Junction / Q000502:physiology* / Q000648:ultrastructure*; D009473:Neuronal Plasticity; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis; D013569:Synapses / Q000502:physiology* / Q000648:ultrastructure*; D014162:Transfection; D025521:Tumor Suppressor Proteins
chemical_list: D015816:Cell Adhesion Molecules, Neuronal; D029721:Drosophila Proteins; D019476:Insect Proteins; D011993:Recombinant Fusion Proteins; D025521:Tumor Suppressor Proteins; C051642:fasciclin II; C070350:dlg1 protein, Drosophila