A charged-particle microbeam: I. Development of an experimental system for targeting cells individually with counted particles.
Charged-particle microbeams provide a unique opportunity to control precisely, the dose to individual cells and the localization of dose within the cell. The Gray Laboratory is now routinely operating a charged-particle microbeam capable of delivering targeted and counted particles to individual cells, at a dose-rate sufficient to permit a number of single-cell assays of radiation damage to be implemented. By this means, it is possible to study a number of important radiobiological processes in ways that cannot be achieved using conventional methods. This report describes the rationale, development and current capabilities of the Gray Laboratory microbeam.
Folkard
M
M
Gray Laboratory Cancer Research Trust, Mount Vernon Hospital, Northwood, Middlesex, UK.
Vojnovic
B
B
Prise
K M
KM
Bowey
A G
AG
Locke
R J
RJ
Schettino
G
G
Michael
B D
BD
D004247:DNA / Q000528:radiation effects; D004249:DNA Damage; D010315:Particle Accelerators / Q000295:instrumentation*; D011853:Radiobiology / Q000295:instrumentation* / Q000379:methods
D004247:DNA