title: Redox-dependent conformational changes are common structural features of cytochrome c from various species.
abstract: Discrepant results from X-ray crystallographic and physicochemical studies on the conformations of the two redox states of cytochrome c raise important questions about the nature of redox-dependent conformational changes and whether differences are common structural features of various cytochrome c species. Comparative studies of cytochrome c from 10 species (horse, cow, sheep, pig, dog, rabbit, chicken, pigeon, tuna, and baker's yeast) in aqueous solutions were carried out using Fourier transform infrared (FT-IR) spectroscopy. The second-derivative analysis revealed similar conformational changes in all 10 species upon reduction of the heme iron regardless of the differences in the amino acid sequences. The redox-dependent changes involve the amide I regions ascribed to extended beta-structure, beta-turn, and alpha-helix structures. Three species (cow, sheep, and pig) with identical amino acid sequences displayed nearly identical infrared spectra for the oxidized and reduced states, which rules out the possible contribution of experimental error. These results show unequivocally that redox-dependent conformational changes are common structural feature of various cytochrome c species and demonstrate the usefulness of FT-IR spectroscopy as a quick and inexpensive tool in comparative studies of functionally related conformational changes of proteins.
authors.affiliation: Department of Chemistry and Biochemistry, University of Northern Colorado, Greeley 80639, USA.
authors.forename: J F
authors.initials: JF
authors.lastname: Calvert
authors.forename: J L
authors.initials: JL
authors.lastname: Hill
authors.forename: A
authors.initials: A
authors.lastname: Dong
mesh_terms: D000818:Animals; D003574:Cytochrome c Group / Q000737:chemistry*; D005399:Fishes; D008322:Mammals; D008958:Models, Molecular; D010084:Oxidation-Reduction; D011487:Protein Conformation; D017433:Protein Structure, Secondary; D012441:Saccharomyces cerevisiae / Q000737:chemistry; D013045:Species Specificity; D017550:Spectroscopy, Fourier Transform Infrared
chemical_list: D003574:Cytochrome c Group