In vivo evaluation of sixteen plant extracts on mice inoculated with Trypanosoma brucei gambiense.
After examination of the drugs used by traditional practitioners in Côte d'lvoire, nine formulas prescribed in the treatment of African human trypanosomiasis (AHT) were selected for investigation. These formulas made use of 40 plants, 16 of which were studied because of their properties, as described in the literature, and their frequent use by practitioners. The plant extracts were administered, after maceration or decoction, either orally or intraperitoneally to Swiss mice that had previously been inoculated with Trypanosoma brucei gambiense (Tbg), strain MHOM/Cl/81/Dal 083. The parasitaemia in each mouse was followed for three consecutive days and compared with that in control mice, which had been given either a saline solution (SS: negative control) or well-known drugs (melarsoprol, difluoromethylornithine, and pentamidine: positive control). Our investigations led to the following conclusions. (a) None of the plant extracts revealed trypanocidal or trypanostatic activity relative to SS controls (P > 0.05). In fact, the mice that received the extracts died on the third day after inoculation, with 0% survival and an average parasitaemia of 10.8 +/- 2 x 10(7) trypanosomes/ml. (b) The treated positive controls, relative to SS, showed 100% survival and no parasitaemia (P < 0.05). Melarsoprol appeared to be active when given orally at a dose of 3.6 mg/kg body weight twice a day for 3 days. This method of testing the sensitivity of trypanosomes to plant extracts is easy and inexpensive, and could be applied to other areas of research on tropical diseases.
Youan
B B
BB
Department of Pharmaceutical Sciences, School of Pharmacy, Catholic University of Louvain, Brussels, Belgium.
Coulibaly
S
S
Miezan
T B
TB
Doua
F
F
Bamba
M
M
D000284:Administration, Oral; D000818:Animals; D007560:Cote d'Ivoire; D000518:Eflornithine / Q000627:therapeutic use; D007263:Infusions, Parenteral; D007274:Injections, Intraperitoneal; D008519:Medicine, Traditional; D008549:Melarsoprol / Q000627:therapeutic use; D051379:Mice; D010419:Pentamidine / Q000627:therapeutic use; D010936:Plant Extracts / Q000008:administration & dosage / Q000627:therapeutic use*; D012965:Sodium Chloride / Q000008:administration & dosage; D014344:Trypanocidal Agents / Q000627:therapeutic use; D014347:Trypanosoma brucei gambiense; D014353:Trypanosomiasis, African / Q000188:drug therapy* / Q000401:mortality
D010936:Plant Extracts; D014344:Trypanocidal Agents; D012965:Sodium Chloride; D010419:Pentamidine; D008549:Melarsoprol; D000518:Eflornithine