title: Identification of three allatostatins and their cDNA from the mosquito Aedes aegypti.
abstract: Three allatostatins have been isolated from the mosquito Aedes aegypti. These peptides have the following structures: Ser-Pro-Lys-Tyr-Asn-Phc-Gly-Leu-amide, Leu-Pro-His-Tyr-Asn-Phe-Gly-Leu-amide, and Arg-Val-Tyr-Asp-Phe-Gly-Leu-amide. A cDNA encoding these peptides was isolated from an abdominal ganglia cDNA library and sequenced. It was found to encode two additional allatostatins: Ala-Ser-Ala-Tyr-Arg-Tyr-His-Phe-Gly-Leu-amide and Leu-Pro-Asn-Arg-Tyr-Asn-Phe-Gly-Leu-amide. Northern analysis of whole mosquito mRNA revealed a single prepro-allatostatin message of around 3,000 bases. Identification of a partial prepro-allatostatin cDNA from a midgut cDNA library shows that the same gene is also expressed in the mosquito midgut.
authors.affiliation: Department of Entomology, University of Arizona, Tucson 85721, USA. veenstra@invertebre.u-bordeaux.fr
authors.forename: J A
authors.initials: JA
authors.lastname: Veenstra
authors.forename: F G
authors.initials: FG
authors.lastname: Noriega
authors.forename: R
authors.initials: R
authors.lastname: Graf
authors.forename: R
authors.initials: R
authors.lastname: Feyereisen
mesh_terms: D000330:Aedes / Q000235:genetics*; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D017931:DNA Primers / Q000235:genetics; D018076:DNA, Complementary / Q000235:genetics*; D008969:Molecular Sequence Data; D009479:Neuropeptides / Q000235:genetics*; D012333:RNA, Messenger / Q000235:genetics; D015183:Restriction Mapping
chemical_list: D017931:DNA Primers; D018076:DNA, Complementary; D009479:Neuropeptides; D012333:RNA, Messenger; C076523:allatostatin