Genomic localization and nucleotide sequence of a lef-8 gene of the Spodoptera littoralis nucleopolyhedrovirus.
A gene similar to lef-8 of the Autographa californica (Ac) nucleopolyhedrovirus (MNPV) was identified in the Spodoptera littoralis (Spli) MNPV. The SpliMNPV lef-8-like gene was localized on the genomic map between 26.9 and 29 map units and is flanked by a chitinase gene and p47 gene. This gene arrangement differs from that of similar genes in the AcMNPV genome, where the lef-8 gene is located about 62 kbp from the chitinase gene and about 7 kbp from the p47 gene. Sequence analyses of the lef-8 gene revealed an ORF of 2730 nucleotides, predicted to encode a protein with Mr 104876. The putative protein is 60.9% identical to the AcMNPV LEF-8 and contains an identical sequence of a conserved motif of DNA-dependent RNA polymerases. Sequences downstream of the lef-8 gene contain two sequence repeats which resemble a repeated motif of the SpliMNPV enhancer element and other repetitive sequences from the viral genome.
Faktor
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O
Department of Entomology, Faculty of Agriculture, The Hebrew University of Jerusalem, Rehovot, Israel.
Kamensky
B
B
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002688:Chitinases / Q000235:genetics; D002874:Chromosome Mapping; D005814:Genes, Viral / Q000235:genetics*; D016679:Genome, Viral; D007814:Larva / Q000821:virology; D008969:Molecular Sequence Data; D017924:Nucleopolyhedroviruses / Q000737:chemistry / Q000235:genetics*; D016366:Open Reading Frames; D012091:Repetitive Sequences, Nucleic Acid; D017386:Sequence Homology, Amino Acid; D012689:Sequence Homology, Nucleic Acid; D018411:Spodoptera / Q000235:genetics; D014764:Viral Proteins / Q000235:genetics*; D015678:Viral Structural Proteins / Q000235:genetics*
D014764:Viral Proteins; D015678:Viral Structural Proteins; C087702:late expression factor 8, Baculovirus; D002688:Chitinases