title: Periosteal new bone formation in a canine neuropathic model of osteoarthritis.
abstract: OBJECTIVE
  To characterize, for the first time, periosteal new bone formation in a well-established canine model of accelerated osteoarthritis (OA) with features of neuropathic arthropathy.
  
  
  METHODS
  Seven dogs underwent left L4-S1 dorsal root ganglionectomy (DRG), followed 3 weeks later by transection of the anterior cruciate ligament of the ipsilateral knee (ACLT). Eight weeks thereafter, a postmortem examination was performed to assess the severity of cartilage changes of OA and the formation of new bone on the distal femur and proximal tibia in the cruciate-deficient limb.
  
  
  RESULTS
  As described previously, extensive full-thickness ulceration of the articular cartilage was present in the unstable knee of every dog. The femoral shaft immediately proximal to the condyles in the unstable limb was consistently wider (mean +/- SD diameter 22.4 +/- 2.2 mm) than that in the contralateral limb (19.9 +/- 1.3 mm; P = 0.01). Xeroradiography and histologic examination of the distal femur revealed extensive formation of woven bone on the periosteal surfaces of the medial, lateral, and anterior aspects of the femoral shaft in the OA limb of every dog. These bony changes were not seen in radiographs of dogs that underwent DRG with the cruciate ligament left intact (n = 8) or of neurologically intact dogs that underwent ACLT (n = 7) and were examined 24 weeks after surgery.
  
  
  CONCLUSIONS
  Formation of new periosteal bone on the distal femur and tibia is a feature of this model of accelerated OA that is not seen in the conventional ACLT model of OA in the neurologically intact dog. This observation suggests that interruption of sensory input from the limb may affect the regulation of osteogenesis in the mechanically unstable joint.
authors.affiliation: Indiana University School of Medicine, and Indiana University Multipurpose Arthritis and Musculoskeletal Diseases Center, Indianapolis, USA.
authors.forename: S L
authors.initials: SL
authors.lastname: Myers
authors.forename: K D
authors.initials: KD
authors.lastname: Brandt
authors.forename: B
authors.initials: B
authors.lastname: O'Connor
authors.forename: W R
authors.initials: WR
authors.lastname: Widmer
authors.forename: M
authors.initials: M
authors.lastname: Albrecht
mesh_terms: D000818:Animals; D004285:Dogs; D005269:Femur / Q000000981:diagnostic imaging / Q000473:pathology; D015171:Ganglionectomy; D008297:Male; D009422:Nervous System Diseases / Q000150:complications*; D010003:Osteoarthritis / Q000000981:diagnostic imaging / Q000209:etiology* / Q000503:physiopathology*; D010012:Osteogenesis / Q000502:physiology*; D010521:Periosteum / Q000000981:diagnostic imaging / Q000503:physiopathology*; D014986:Xeroradiography