Efficiency of neural differentiation of mouse P19 embryonal carcinoma cells is dependent on the seeding density.
Serum-free culture conditions for retinoic acid-induced neural differentiation of mouse P19 embryonal carcinoma cells were determined for future ex vivo retroviral gene transfer and brain transplantation studies. Neural differentiation of P19 cells was dependent on the seeding densities, and both neurons and astroglia differentiated efficiently at high seeding densities (2 x 10(4) and 5 x 10(4) cells/cm2) but not at low seeding density (1 x 10(4) cells/cm2). In addition, P19 cells cultured at 5 x 10(4) cells/cm2 showed neural differentiated whether or not they were infected with Friend leukemia virus FrC6-V, which inhibited neural differentiation at 2 x 10(4) cells/cm2. These results indicate that FrC6-V-infected P19 embryonal carcinoma cells should be seeded at high density to achieve efficient neural differentiation in vitro for ex vivo gene transfer with a FrC6-V-derived retroviral vector system.
Kitani
H
H
National Institute of Animal Health, Ibaraki, Japan.
Ikeda
H
H
Atsumi
T
T
Watanabe
R
R
D000818:Animals; D001253:Astrocytes / Q000166:cytology*; D002452:Cell Count; D002454:Cell Differentiation / Q000187:drug effects; D016895:Culture Media, Serum-Free; D054278:Embryonal Carcinoma Stem Cells; D005622:Friend murine leukemia virus / Q000502:physiology; D005822:Genetic Vectors; D005904:Glial Fibrillary Acidic Protein / Q000032:analysis; D051379:Mice; D014411:Neoplastic Stem Cells / Q000166:cytology* / Q000821:virology; D016900:Neurofilament Proteins / Q000032:analysis; D009474:Neurons / Q000166:cytology*; D014212:Tretinoin / Q000494:pharmacology; D014407:Tumor Cells, Cultured
D016895:Culture Media, Serum-Free; D005904:Glial Fibrillary Acidic Protein; D016900:Neurofilament Proteins; D014212:Tretinoin