title: Mouse lemur microscopic MRI brain atlas.
abstract: We present a three-dimensional (3D) digital atlas of a mouse lemur head at 60-micron cubic voxel isotropic resolution. It was constructed from a 3D proton magnetic resonance image (MRI) acquired at 500 MHz. It shows views of 3D volume-rendering and movies of contiguous 2D plane cuts in three orthogonal directions. This MR data set was acquired using a spin-echo pulse sequence under conditions of strong T2 and significant diffusion weighting. Experimental parameters were optimized to provide strong intrinsic contrast between gray and white matter. Familiar anatomical structures are clearly identifiable. Fine fiber tracts, laminations of cortices, details of the inner ears, and layering in the lateral geniculate nuclei are all visible. Anatomical identifications of structures in representative slices selected from the atlas are presented. We also describe some difficulties and trade-offs encountered in microscopic resolution MRI. We note that this type of atlas does not suffer from the spatial distortions and slice registration problems introduced by standard histological techniques. Future in vivo longitudinal studies will provide atlases describing in detail the development of the primate brain.
authors.affiliation: Beckman Institute, Division of Biology, California Institute of Technology, Pasadena 91125, USA.
authors.forename: P
authors.initials: P
authors.lastname: Ghosh
authors.forename: M
authors.initials: M
authors.lastname: O'Dell
authors.forename: P T
authors.initials: PT
authors.lastname: Narasimhan
authors.forename: S E
authors.initials: SE
authors.lastname: Fraser
authors.forename: R E
authors.initials: RE
authors.lastname: Jacobs
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D001921:Brain / Q000033:anatomy & histology*; D001931:Brain Mapping / Q000295:instrumentation*; D016251:CD-ROM; D016565:Cheirogaleidae / Q000033:anatomy & histology*; D003199:Computer Systems; D007091:Image Processing, Computer-Assisted / Q000295:instrumentation*; D008279:Magnetic Resonance Imaging / Q000295:instrumentation*