Identification of strain-specific nucleotide sequences in E1 and NS4 genes of rubella virus vaccine strains in Japan.
Strain-specific nucleotide sequences of E1 and NS4 genes in five strains of a live rubella virus vaccine manufactured in Japan were identified for comparison, using 2389 nucleotides (1443 nucleotides of the E1 gene, 41 of the 3' terminal region following the E1 gene and 905 of the NS4 gene). Sequences of the E1 gene in three strains (Matsuura, TCRB19 and To-336) were identified. Takahashi and Matsuba strains shared common sequences, but were discriminated by the sequence of the NS4 gene. These five strains showed a phylogenetic relationship with the places of their isolation. In a comparative study of three strains with their unattenuated progenitors, the nucleotides in these regions were almost conserved during the attenuation process.
Katow
S
S
Department of Viral Disease and Vaccine Control, National Institute of Health, Tokyo, Japan.
Minahara
H
H
Ota
T
T
Fukushima
M
M
D001483:Base Sequence; D005814:Genes, Viral; D007564:Japan; D008969:Molecular Sequence Data; D012411:Rubella Vaccine / Q000235:genetics*; D012412:Rubella virus / Q000145:classification / Q000235:genetics*; D013045:Species Specificity; D014759:Viral Envelope Proteins / Q000235:genetics*; D017361:Viral Nonstructural Proteins / Q000235:genetics*
D012411:Rubella Vaccine; D014759:Viral Envelope Proteins; D017361:Viral Nonstructural Proteins; C061826:E1 envelope protein, Rubella virus