title: Applications of the green fluorescent protein in cell biology and biotechnology.
abstract: The recent emergence of an autofluorescent protein, the green fluorescent protein (GFP), has opened the door for the convenient use of intact living cells and organisms as experimental systems in fields ranging from cell biology to biomedicine. We present an overview of some of the major applications of GFP, namely its use in protein tagging and in monitoring gene expression as well as its potential in a variety of biological screens.
authors.affiliation: Cold Spring Harbor Laboratory, NY 11724, USA. misteli@cshl.org
authors.forename: T
authors.initials: T
authors.lastname: Misteli
authors.forename: D L
authors.initials: DL
authors.lastname: Spector
mesh_terms: D000818:Animals; D001709:Biotechnology / Q000639:trends; D002477:Cells / Q000737:chemistry* / Q000378:metabolism; D004330:Drosophila / Q000235:genetics; D005434:Flow Cytometry; D005786:Gene Expression Regulation / Q000235:genetics*; D005818:Genetic Engineering / Q000639:trends; D015316:Genetic Therapy / Q000639:trends; D049452:Green Fluorescent Proteins; D008164:Luminescent Proteins / Q000737:chemistry* / Q000235:genetics* / Q000502:physiology; D009154:Mutation / Q000235:genetics; D037401:Scyphozoa / Q000502:physiology; D015398:Signal Transduction / Q000235:genetics
chemical_list: D008164:Luminescent Proteins; D049452:Green Fluorescent Proteins