title: Evaluation of the automated COBAS AMPLICOR hepatitis C virus PCR system.
abstract: To evaluate the reliability and feasibility of the automated Roche COBAS AMPLICOR PCR system for routine detection of hepatitis C virus (HCV) RNA, a total of 405 serum samples previously tested by an in-house nested PCR and manual Roche AMPLICOR microwell plate HCV test were examined. Complete concordance was found between the results with the HCV COBAS AMPLICOR system and the previously determined HCV RNA status. The automated HCV COBAS AMPLICOR system provides the clinical microbiology laboratory with a specific and sensitive PCR method for rapid and reliable detection of HCV RNA.
authors.affiliation: Institute of Microbiology and Immunology, Medical Faculty, Ljubljana, Slovenia. poljak@ibmi.mf.uni-lj.si
authors.forename: M
authors.initials: M
authors.lastname: Poljak
authors.forename: K
authors.initials: K
authors.lastname: Seme
authors.forename: S
authors.initials: S
authors.lastname: Koren
mesh_terms: D001331:Automation / Q000295:instrumentation / Q000379:methods; D005240:Feasibility Studies; D016174:Hepacivirus / Q000302:isolation & purification*; D006526:Hepatitis C / Q000097:blood / Q000175:diagnosis*; D006801:Humans; D016133:Polymerase Chain Reaction / Q000295:instrumentation* / Q000379:methods; D012367:RNA, Viral / Q000097:blood*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
chemical_list: D012367:RNA, Viral