title: Gene expression systems in the development of high-throughput screens.
abstract: Recent advances in the development of combinatorial automated chemical synthesis, robotic sample handling, and data collection and analysis have significantly increased the number of compounds available for screening against potential therapeutic targets. The implementation of highly sensitive in vitro biochemical and cell-based high-throughput screening assays is essential to facilitate the rapid identification of selective and potent lead molecules from compound libraries. The ability to easily produce functional proteins in sufficient quantities for in vitro biochemical assays and to devise useful cell-based systems is dependent on the successful application of a variety of gene expression systems.
authors.affiliation: Receptor Biochemistry and Molecular Sciences Department, Glaxo Wellcome Research and Development, Research Triangle Park, NC 27709, USA. jayawickreme-ck@glaxo.com
authors.forename: C K
authors.initials: CK
authors.lastname: Jayawickreme
authors.forename: T A
authors.initials: TA
authors.lastname: Kost
mesh_terms: D000818:Animals; D018929:Cell Culture Techniques / Q000379:methods; D002460:Cell Line; D003001:Cloning, Molecular / Q000379:methods; D003577:Cytochrome P-450 Enzyme System / Q000096:biosynthesis; D015870:Gene Expression; D008024:Ligands; D008322:Mammals; D008547:Melanophores / Q000166:cytology; D011956:Receptors, Cell Surface / Q000096:biosynthesis / Q000502:physiology; D011994:Recombinant Proteins / Q000096:biosynthesis*; D012441:Saccharomyces cerevisiae; D014162:Transfection / Q000379:methods; D015262:Xenobiotics / Q000378:metabolism; D014982:Xenopus laevis
chemical_list: D008024:Ligands; D011956:Receptors, Cell Surface; D011994:Recombinant Proteins; D015262:Xenobiotics; D003577:Cytochrome P-450 Enzyme System