title: Immunolocalization of CD44 and the ezrin-radixin-moesin (ERM) family in the stratum intermedium and papillary layer of the mouse enamel organ.
abstract: We studied the immunohistochemical localization of CD44 and the ezrin-radixin-moesin (ERM) family of actin binding proteins in mouse enamel organ, using confocal laser scanning microscopy and transmission electron microscopy to clarify their role in cytoskeletal organization. At the differentiation stage of ameloblasts, immunoreactivity to CD44 was detected on the plasma membrane of the inner enamel epithelium, the stellate reticulum, the stratum intermedium, and the external enamel epithelium. In accordance with the differentiation of preameloblasts into secretory ameloblasts, immunoreactivity increased in the stratum intermedium cells. At the maturation stage, intense immunoreactivity was observed on the papillary layer cells. For the ERM family, the stratum intermedium and the papillary layer cells were stained with anti-ezrin and -radixin monoclonal antibodies but not with the anti-moesin antibody. Electron microscopic observations revealed that CD44, ezrin, and radixin were localized in the region at which preameloblasts came into contact with the stratum intermedium at the differentiation stage. At the secretory and maturation stages, they were concentrated in the microvilli of the stratum intermedium and the papillary layer cells. These findings suggest that the CD44-ezrin-radixin-actin filament system is involved in cell-cell interaction between preameloblasts and the stratum intermedium, and in the cytoskeletal organization of the cells in the stratum intermedium and the papillary layer.
authors.affiliation: Department of Oral Anatomy, Niigata University School of Dentistry, Japan.
authors.forename: H
authors.initials: H
authors.lastname: Nakamura
authors.forename: H
authors.initials: H
authors.lastname: Ozawa
mesh_terms: D000565:Ameloblasts / Q000737:chemistry; D000818:Animals; D001798:Blood Proteins / Q000032:analysis*; D003598:Cytoskeletal Proteins; D004658:Enamel Organ / Q000737:chemistry* / Q000254:growth & development; D019085:Fluorescent Antibody Technique, Direct; D018960:Hyaluronan Receptors / Q000032:analysis*; D008297:Male; D008565:Membrane Proteins / Q000032:analysis*; D051379:Mice; D008815:Mice, Inbred Strains; D008840:Microfilament Proteins; D018613:Microscopy, Confocal; D008854:Microscopy, Electron; D010750:Phosphoproteins / Q000032:analysis*; D011506:Proteins / Q000032:analysis*; D000099149:Ezrin
chemical_list: D001798:Blood Proteins; D003598:Cytoskeletal Proteins; D018960:Hyaluronan Receptors; D008565:Membrane Proteins; D008840:Microfilament Proteins; D010750:Phosphoproteins; D011506:Proteins; D000099149:Ezrin; C071058:moesin; C071776:radixin