title: Combined flow cytometry and immunohistochemistry analyses for the assessment of nasopharyngeal carcinoma cell kinetics by in vivo bromodeoxyuridine infusion.
abstract: Thirty-eight previously untreated patients with a histologically proved diagnosis of nasopharyngeal carcinoma were evaluated for in vivo cell kinetics before treatment by conventional radiation therapy. Thirty-seven tumors were analyzed by flow cytometry. Values of median potential doubling time (Tpot), labelling index (LI), and duration of S phase (Ts) were, respectively, 10.9 days, 3.8%, and 10.8 hours. In 35 cases, the results obtained from two biopsies of the same tumor were compared. A good reproducibility was obtained for LI and Tpot (P < 0.01). Thirty-one tumors were analysed by immunohistochemistry and labelling index (HLI) was determined in 24 tumors with a percentage of labelled cells varying from 6.1% to 39.2% (median value = 18.5%). No correlation was found between LI and HLI, but when observations were focused on the restricted group of DNA aneuploid samples, mean values of LI and HLI were closer (respectively, 12.8 +/- 4.5% and 18.3 +/- 7.7%) and a good correlation was obtained (P = 0.01). Moreover, no difference in proliferation was found between diploid and aneuploid tumors. Considering these results, a combined Tpot was calculated that allowed classification of tumors as highly or slowly proliferative.
authors.affiliation: Laboratoire de Recherche en Oncologie, Centre Alexis Vautrin, Vandoeuvre-les-Nancy, France. smarchal@nancy.fnclcc.fr
authors.forename: S
authors.initials: S
authors.lastname: Marchal
authors.forename: C
authors.initials: C
authors.lastname: Marchal
authors.forename: R M
authors.initials: RM
authors.lastname: Parache
authors.forename: N
authors.initials: N
authors.lastname: Benjaafar
authors.forename: M
authors.initials: M
authors.lastname: Odda
authors.forename: A
authors.initials: A
authors.lastname: Leclerc
authors.forename: J L
authors.initials: JL
authors.lastname: Merlin
authors.forename: B
authors.initials: B
authors.lastname: el Gueddarri
authors.forename: P
authors.initials: P
authors.lastname: Bey
mesh_terms: D000328:Adult; D000368:Aged; D001973:Bromodeoxyuridine / Q000008:administration & dosage*; D002277:Carcinoma / Q000378:metabolism / Q000473:pathology*; D005434:Flow Cytometry / Q000379:methods*; D006801:Humans; D007150:Immunohistochemistry / Q000379:methods*; D007262:Infusions, Intravenous; D007700:Kinetics; D008875:Middle Aged; D009303:Nasopharyngeal Neoplasms / Q000378:metabolism / Q000473:pathology*; D015203:Reproducibility of Results
chemical_list: D001973:Bromodeoxyuridine