Anti-tumor activity of squid ink.
The anti-tumor activity of a new type of peptidoglycan isolated from squid ink was shown to have a cure rate of 64% for Meth A tumor from BALB/c mice. The ink delipidated in acetone, which contained the peptidoglycan at 0.1% (w/w), was administered to tumor-transplanted mice so as to examine the anti-tumor activity. One-fifth of the tumor-bearing mice was cured with 3 injections (1 mg/head) of the acetone delipidated squid ink or a prolongation of survival was observed in the treated animals. Heat treatment at 100 degrees C for 10 min did not affect the anti-tumor activity of the delipidated ink, its potentiality being preserved. The acetone-extractable fraction of the ink also brought about a similar cure rate for Meth A tumor. The delipidated ink enhanced the phagocytic activity of macrophages but no direct cytotoxicity was observed for the Meth A tumor cells. Hence it may be said that the anti-tumor activity of the delipidated ink was mainly due to the augmented cellular immunity in vivo.
Sasaki
J
J
Department of Bacteriology, Hirosaki University School of Medicine, Japan.
Ishita
K
K
Takaya
Y
Y
Uchisawa
H
H
Matsue
H
H
D000096:Acetone; D000116:Acetylgalactosamine / Q000032:analysis; D000818:Animals; D000970:Antineoplastic Agents / Q000627:therapeutic use*; D002273:Carcinogens; D049832:Decapodiformes / Q000378:metabolism*; D004355:Drug Stability; D005354:Fibrosarcoma / Q000139:chemically induced / Q000188:drug therapy*; D005643:Fucose / Q000032:analysis; D005965:Glucuronates / Q000032:analysis; D020723:Glucuronic Acid; D006358:Hot Temperature; D008055:Lipids; D008543:Melanins / Q000032:analysis; D051379:Mice; D008807:Mice, Inbred BALB C; D009368:Neoplasm Transplantation; D010457:Peptidoglycan / Q000032:analysis / Q000302:isolation & purification / Q000627:therapeutic use*; D011134:Polysaccharides / Q000032:analysis
D000970:Antineoplastic Agents; D002273:Carcinogens; D005965:Glucuronates; D008055:Lipids; D008543:Melanins; D010457:Peptidoglycan; D011134:Polysaccharides; D000096:Acetone; D005643:Fucose; D020723:Glucuronic Acid; D000116:Acetylgalactosamine