title: Protein memory through altered folding mediated by intramolecular chaperones.
abstract: The 77-residue propeptide of subtilisin acts as an intramolecular chaperone that organizes the correct folding of its own protease domain. Similar folding mechanisms are used by several prokaryotic and eukaryotic proteins, including prohormone-convertases. Here we show that the intramolecular chaperone of subtilisin facilitates folding by acting as a template for its protease domain, although it does not form part of that domain. Subtilisin E folded by an intramolecular chaperone with an Ile(-48)-to-Val mutation acquires an 'altered' enzymatically active conformation that differs from wild-type subtilisin E. Although both the altered and wild-type subtilisins have identical amino-acid sequences, as determined by amino-terminal sequencing and mass spectrometry, they bind their cognate intramolecular chaperones with 4.5-fold greater affinity than non-cognate intramolecular chaperones, when added in trans. The two subtilisins also have different secondary structures, thermostability and substrate specificities. Our results indicate that an identical polypeptide can fold into an altered conformation through a mutated intramolecular chaperone and maintains memory of the folding process. Such a phenomenon, which we term 'protein memory', may be important in investigations of protein folding.
authors.affiliation: Department of Biochemistry, Robert Wood Johnson Medical School-UMDNJ, Piscataway, New Jersey 08854, USA.
authors.forename: U P
authors.initials: UP
authors.lastname: Shinde
authors.forename: J J
authors.initials: JJ
authors.lastname: Liu
authors.forename: M
authors.initials: M
authors.lastname: Inouye
mesh_terms: D001665:Binding Sites; D002384:Catalysis; D004795:Enzyme Stability; D004926:Escherichia coli; D018832:Molecular Chaperones / Q000502:physiology*; D017510:Protein Folding; D011994:Recombinant Proteins / Q000737:chemistry; D013381:Subtilisins / Q000737:chemistry* / Q000235:genetics / Q000502:physiology
chemical_list: D018832:Molecular Chaperones; D011994:Recombinant Proteins; D013381:Subtilisins