title: In utero undernutrition impairs rat beta-cell development.
abstract: The role of nutrition on the development of the endocrine pancreas was studied in a rat model obtained by maternal food restriction. A 50% food restriction was applied to female rats from day 15 of pregnancy and resulted in intrauterine growth-retardation (IUGR) in the offspring. At day 1 postnatal, beta-cell mass was significantly decreased in IUGR pups as compared to controls (0.70 +/- 0.06 vs 1.07 +/- 0.06 mg, p < 0.0001), as well as insulin content. This change in beta-cell mass can be attributed to a reduced number of islets, since the density of insulin-positive aggregates in pancreatic sections of IUGR rats was 20% lower than in controls. Proliferative capacity of beta cells, as measured by 5-bromo-2-deoxyuridine (BrdU) labelling index, was not altered in growth-retarded animals. Body as well as pancreatic weight were fully recovered in IUGR pups after 21 days of normal feeding by control mothers. However, these animals retained a 25% decrease in insulin content, 40% decrease in beta-cell mass (1.58 +/- 0.18 vs 2.78 +/- 0.42 mg, p < 0.001) and a strong reduction in the density of insulin positive aggregates per cm2, as compared to controls, suggesting that the total islet number was likely to be reduced. Beta-cell proliferative capacity remained normal. In conclusion, in utero undernutrition in rats does not impede postnatal growth but durably impairs beta-cell development. Impairment of beta-cell differentiation might be suggested.
authors.affiliation: INSERM U 457, Hôpital Robert Debré, Paris, France.
authors.forename: A
authors.initials: A
authors.lastname: Garofano
authors.forename: P
authors.initials: P
authors.lastname: Czernichow
authors.forename: B
authors.initials: B
authors.lastname: Bréant
mesh_terms: D000824:Animal Nutritional Physiological Phenomena; D000818:Animals; D005260:Female; D005317:Fetal Growth Retardation / Q000196:embryology*; D007150:Immunohistochemistry; D007515:Islets of Langerhans / Q000254:growth & development*; D051379:Mice; D009748:Nutrition Disorders / Q000196:embryology*; D011247:Pregnancy; D051381:Rats; D017208:Rats, Wistar