Cyclic peptides as conformationally restricted models of viral antigens: application to foot-and-mouth disease virus.
Conformationally restricted cyclic peptide mimics of the antigenic site A of foot-and-mouth disease virus serotype C-S8c1 have been designed, first by comparison to the three-dimensional structure of the O1BFS serotype, later more accurately on the basis of X-ray diffraction data from a complex between a linear peptide reproducing site A and an FMDV-derived monoclonal antibody Fab fragment. A variety of cyclization strategies have been attempted, both in solution and in the solid phase, involving disulfide, side chain lactam and head-to-tail arrangements. Preliminary immunological results have shown one of the cyclic disulfide mimics to be a better immunogen than its linear counterpart.
Valero
M L
ML
Department of Organic Chemistry, University of Barcelona, Spain.
Camarero
J A
JA
Adeva
A
A
Verdaguer
N
N
Fita
I
I
Mateu
M G
MG
Domingo
E
E
Giralt
E
E
Andreu
D
D
D000595:Amino Acid Sequence; D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D000956:Antigens, Viral / Q000737:chemistry* / Q000276:immunology; D005537:Aphthovirus / Q000276:immunology*; D002851:Chromatography, High Pressure Liquid; D007140:Immunoglobulin Fab Fragments / Q000737:chemistry* / Q000276:immunology; D051379:Mice; D008958:Models, Molecular; D018716:Molecular Mimicry / Q000276:immunology*; D008969:Molecular Sequence Data; D010456:Peptides, Cyclic / Q000032:analysis / Q000737:chemistry* / Q000276:immunology
D000911:Antibodies, Monoclonal; D000956:Antigens, Viral; D007140:Immunoglobulin Fab Fragments; D010456:Peptides, Cyclic