Immunohistological assessment of fibrin deposition and thrombus formation in canine mammary neoplasia.
A commercially available monoclonal antibody against human fibrin was used to detect fibrin in canine formalin-fixed, paraffin wax-embedded tissue by applying a slightly modified alkaline phosphatase anti-alkaline phosphatase (APAAP) technique. Twenty-eight mammary tumours from six bitches were examined for the presence of fibrin. Thrombi and extravascular fibrin deposits were detected in 15 tumours (12 complex adenocarcinomas, one adenocarcinoma, two solid carcinomas), and a single thrombus was detected in one adenoma; 12 tumours (three adenomas, one complex adenoma, four complex adenocarcinomas and four adenocarcinomas) did not show any staining reaction.
Golombiewski
A
A
Institut für Veterinär-Pathologie, Freie Universität Berlin, Germany.
Gutberlet
K
K
Rudolph
R
R
D000230:Adenocarcinoma / Q000737:chemistry; D000236:Adenoma / Q000737:chemistry; D000818:Animals; D000911:Antibodies, Monoclonal; D002277:Carcinoma / Q000737:chemistry; D004285:Dogs; D005260:Female; D005337:Fibrin / Q000032:analysis* / Q000276:immunology; D007150:Immunohistochemistry; D015674:Mammary Neoplasms, Animal / Q000737:chemistry* / Q000473:pathology*; D009336:Necrosis; D013927:Thrombosis / Q000473:pathology*
D000911:Antibodies, Monoclonal; D005337:Fibrin