Xenopus FK 506-binding protein homolog induces a secondary axis in frog embryos, which is inhibited by coexisting BMP 4 signaling.
FK 506-binding protein (FKBP) is an immunosuppressant mediator in mammals, but its endogenous physiological function has yet to be determined. Here we report a Xenopus homolog of FKBP, which is expressed at early stages of development. Injection of synthesized Xenopus FKBP mRNA, as well as murine constitutively active calcineurin, induced a secondary axis in Xenopus embryos, while an FKBP mutant which does not bind to calcineurin did not. This secondary-axis-inducing effect was inhibited when FKBP was coinjected with Xmad 1 or XBMP 4 mRNA. These results suggest that FKBP modifies BMP 4 signalling by recruiting calcineurin and may have an important role in axis formation during Xenopus development.
Nishinakamura
R
R
Institute of Medical Science, University of Tokyo, Japan.
Matsumoto
Y
Y
Uochi
T a
Ta
Asashima
M
M
Yokota
T
T
D000595:Amino Acid Sequence; D000818:Animals; D019521:Body Patterning / Q000187:drug effects* / Q000235:genetics; D055415:Bone Morphogenetic Protein 4; D019485:Bone Morphogenetic Proteins / Q000235:genetics / Q000502:physiology*; D002352:Carrier Proteins / Q000096:biosynthesis / Q000235:genetics / Q000494:pharmacology*; D003001:Cloning, Molecular; D004268:DNA-Binding Proteins / Q000008:administration & dosage / Q000096:biosynthesis / Q000235:genetics / Q000494:pharmacology*; D004625:Embryo, Nonmammalian / Q000187:drug effects* / Q000502:physiology; D006360:Heat-Shock Proteins / Q000096:biosynthesis / Q000235:genetics / Q000494:pharmacology*; D006801:Humans; D051379:Mice; D008845:Microinjections; D008969:Molecular Sequence Data; D012333:RNA, Messenger / Q000008:administration & dosage; D015398:Signal Transduction / Q000187:drug effects* / Q000235:genetics; D051785:Smad Proteins; D022021:Tacrolimus Binding Proteins; D015534:Trans-Activators; D014981:Xenopus / Q000196:embryology*; D029867:Xenopus Proteins
C525809:BMP4 protein, human; C525810:Bmp4 protein, mouse; D055415:Bone Morphogenetic Protein 4; D019485:Bone Morphogenetic Proteins; D002352:Carrier Proteins; D004268:DNA-Binding Proteins; D006360:Heat-Shock Proteins; D012333:RNA, Messenger; D051785:Smad Proteins; D015534:Trans-Activators; D029867:Xenopus Proteins; C525812:bmp4 protein, Xenopus; D022021:Tacrolimus Binding Proteins