Decreased production of interleukin-1-beta, prostaglandin-E2 and thromboxane-B2, and elevated levels of interleukin-6 and -10 are associated with increased survival during endotoxic shock in mice consuming diets enriched with sesame seed oil supplemented with Quil-A saponin.
Sesamin, present in sesame seed oil (SSO), can inhibit delta-5-desaturase activity and cause accumulation of dihomo-gamma-linolenic acid (DGLA), which displaces arachidonic acid, and subsequently decrease production of dienoic eicosanoids. The effects of diets containing both SSO and Quil A, a saponin that emulsifies fats and potentiates the immune responses, were also studied. A mixture of oils having a fatty-acid composition similar to that of SSO served as a control diet. The levels of docosapentaenoic acid in mice fed Quil-A-supplemented diets and of DGLA in those fed SSO diets were markedly higher in the liver. These changes were associated with a significant reduction in the plasma prostaglandin-E(1+2) and thromboxane-B2 levels in response to an intraperitoneal injection of a lethal dose of lipopolysaccharide (LPS) endotoxin (LD50 20 mg/kg). The levels of interleukin (IL-)6 were elevated and those of IL-1beta were decreased in mice consuming Quil-A-supplemented diets. The IL-10 levels that were elevated in all mice after LPS exposure, remained higher (even at 9 h) only in those fed Quil-A-supplemented diets, but declined rapidly in others. During a 48-hour observation period following LPS injection, all control animals died, and survival was 40% in the SSO group, and 27 and 50%, respectively, in those fed Quil-A-supplemented control and SSO diets. These data suggest that SSO and Quil A when present in the diet exerted cumulative effects that resulted in a decrease in the levels of dienoic eicosanoids with a reduction in IL-1beta and a concomitant elevation in the levels of IL-10 that were associated with a marked increase in survival in mice.
Chavali
S R
SR
Department of Surgery, Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, Mass. 02215, USA.
Zhong
W W
WW
Utsunomiya
T
T
Forse
R A
RA
D000818:Animals; D019587:Dietary Supplements; D015232:Dinoprostone / Q000096:biosynthesis; D004149:Dioxoles / Q000494:pharmacology; D015777:Eicosanoids / Q000096:biosynthesis*; D005227:Fatty Acids / Q000032:analysis; D005260:Female; D007378:Interleukins / Q000096:biosynthesis*; D017705:Lignans; D008099:Liver / Q000737:chemistry; D008566:Membranes / Q000737:chemistry; D051379:Mice; D008807:Mice, Inbred BALB C; D062605:Quillaja Saponins; D012503:Saponins / Q000494:pharmacology*; D012715:Sesame Oil / Q000494:pharmacology*; D012772:Shock, Septic / Q000178:diet therapy* / Q000276:immunology / Q000401:mortality; D016019:Survival Analysis; D013929:Thromboxane B2 / Q000096:biosynthesis
D004149:Dioxoles; D015777:Eicosanoids; D005227:Fatty Acids; D007378:Interleukins; D017705:Lignans; D062605:Quillaja Saponins; D012503:Saponins; D013929:Thromboxane B2; C046386:Quil A; D012715:Sesame Oil; D015232:Dinoprostone; C054125:sesamin