Treadmill chamber for studies of respiratory gas exchange in the rat during exercise.
A treadmill for studying gas exchange in small mammals during exercise is presented. The system consists of a motor-driven running mat enclosed in a gastight chamber that receives a measured flow of air from a compressed air cylinder. The gas flow and temperature, pressure and instantaneous gas composition of the chamber (oxygen, carbon dioxide and water) are measured continuously and the data are computed to include the effects on chamber atmosphere of the rat activity, either running or at rest. The system is completed with a shock delivery grid that stimulates the rat to run. The calculations are based on the changes in the composition of the gas in the chamber (constantly stirred by a small electric fan) induced by the rat instead of relying on the alterations induced in the outflowing gas. The consumption of oxygen, and production of carbon dioxide and water by the rat are computed in real time, giving a very fast response to physiological change induced by exercise. The chamber is custom-made from an aluminium block and a plexiglass lid; all other components are available commercially. The system, as described, allows for a detailed analysis of respiratory gas (and water) exchange by rats under varying exercise conditions, there is practically no time lag between changes in respiratory gases and the detection of these changes, and the buffering effect of the chamber size is practically eliminated because of the calculation approach used.
Ardévol
A
A
Departament de Bioquimica i Fisiologia, Facultat de Biologia, Universitat de Barcelona, Spain.
Adán
C
C
Fernández-López
J A
JA
Pérez
J
J
Corts
J L
JL
Binagui
D
D
Remesar
X
X
Alemany
M
M
D000818:Animals; D002245:Carbon Dioxide / Q000032:analysis; D005080:Exercise Test / Q000295:instrumentation* / Q000379:methods; D010100:Oxygen / Q000032:analysis; D010101:Oxygen Consumption; D011312:Pressure; D011659:Pulmonary Gas Exchange; D051381:Rats; D013696:Temperature; D014867:Water / Q000032:analysis
D014867:Water; D002245:Carbon Dioxide; D010100:Oxygen