Ribotyping for strain characterization of Clostridium perfringens isolates from food poisoning cases and outbreaks.
Ribotyping was used to characterize 34 Clostridium perfringens strains isolated from 10 food poisoning cases and outbreaks over a 7-year period. Twelve different ribopatterns were generated by EcoRI digestion. In eight food poisoning cases and outbreaks, all of the ribotypes of each food and stool isolate were found to be identical. Two C. perfringens isolates showed unique patterns. Ribotyping was found to be a useful tool for determining the genetic relationship of C. perfringens isolates in the context of foodborne poisoning cases.
Schalch
B
B
Institute for Hygiene and Technology of Foods of Animal Origin, Veterinary Faculty, Ludwig-Maximilians-University, Munich, Germany.
Björkroth
J
J
Eisgruber
H
H
Korkeala
H
H
Stolle
A
A
D000818:Animals; D015373:Bacterial Typing Techniques; D003015:Clostridium Infections / Q000453:epidemiology / Q000382:microbiology*; D003016:Clostridium perfringens / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification; D004269:DNA, Bacterial / Q000235:genetics / Q000302:isolation & purification; D015246:Deoxyribonuclease EcoRI; D004196:Disease Outbreaks; D005243:Feces / Q000382:microbiology; D005516:Food Microbiology; D005517:Foodborne Diseases / Q000453:epidemiology / Q000382:microbiology*; D005858:Germany / Q000453:epidemiology; D006801:Humans
D004269:DNA, Bacterial; D015246:Deoxyribonuclease EcoRI