title: A charged-particle microbeam: I. Development of an experimental system for targeting cells individually with counted particles.
abstract: Charged-particle microbeams provide a unique opportunity to control precisely, the dose to individual cells and the localization of dose within the cell. The Gray Laboratory is now routinely operating a charged-particle microbeam capable of delivering targeted and counted particles to individual cells, at a dose-rate sufficient to permit a number of single-cell assays of radiation damage to be implemented. By this means, it is possible to study a number of important radiobiological processes in ways that cannot be achieved using conventional methods. This report describes the rationale, development and current capabilities of the Gray Laboratory microbeam.
authors.affiliation: Gray Laboratory Cancer Research Trust, Mount Vernon Hospital, Northwood, Middlesex, UK.
authors.forename: M
authors.initials: M
authors.lastname: Folkard
authors.forename: B
authors.initials: B
authors.lastname: Vojnovic
authors.forename: K M
authors.initials: KM
authors.lastname: Prise
authors.forename: A G
authors.initials: AG
authors.lastname: Bowey
authors.forename: R J
authors.initials: RJ
authors.lastname: Locke
authors.forename: G
authors.initials: G
authors.lastname: Schettino
authors.forename: B D
authors.initials: BD
authors.lastname: Michael
mesh_terms: D004247:DNA / Q000528:radiation effects; D004249:DNA Damage; D010315:Particle Accelerators / Q000295:instrumentation*; D011853:Radiobiology / Q000295:instrumentation* / Q000379:methods
chemical_list: D004247:DNA