title: Metal ion chaperone function of the soluble Cu(I) receptor Atx1.
abstract: Reactive and potentially toxic cofactors such as copper ions are imported into eukaryotic cells and incorporated into target proteins by unknown mechanisms. Atx1, a prototypical copper chaperone protein from yeast, has now been shown to act as a soluble cytoplasmic copper(I) receptor that can adopt either a two- or three-coordinate metal center in the active site. Atx1 also associated directly with the Atx1-like cytosolic domains of Ccc2, a vesicular protein defined in genetic studies as a member of the copper-trafficking pathway. The unusual structure and dynamics of Atx1 suggest a copper exchange function for this protein and related domains in the Menkes and Wilson disease proteins.
authors.affiliation: Department of Chemistry, Northwestern University, Evanston, IL 60208, USA.
authors.forename: R A
authors.initials: RA
authors.lastname: Pufahl
authors.forename: C P
authors.initials: CP
authors.lastname: Singer
authors.forename: K L
authors.initials: KL
authors.lastname: Peariso
authors.forename: S J
authors.initials: SJ
authors.lastname: Lin
authors.forename: P J
authors.initials: PJ
authors.lastname: Schmidt
authors.forename: C J
authors.initials: CJ
authors.lastname: Fahrni
authors.forename: V C
authors.initials: VC
authors.lastname: Culotta
authors.forename: J E
authors.initials: JE
authors.lastname: Penner-Hahn
authors.forename: T V
authors.initials: TV
authors.lastname: O'Halloran
mesh_terms: D000595:Amino Acid Sequence; D002352:Carrier Proteins; D027682:Cation Transport Proteins; D003300:Copper / Q000378:metabolism*; D000079922:Copper Transport Proteins; D004926:Escherichia coli; D005656:Fungal Proteins / Q000378:metabolism / Q000502:physiology*; D006801:Humans; D018832:Molecular Chaperones / Q000502:physiology*; D008969:Molecular Sequence Data; D011994:Recombinant Proteins; D012441:Saccharomyces cerevisiae / Q000378:metabolism / Q000502:physiology*; D029701:Saccharomyces cerevisiae Proteins; D017386:Sequence Homology, Amino Acid
chemical_list: C093341:ATX1 protein, S cerevisiae; C092974:CCC2 protein, S cerevisiae; D002352:Carrier Proteins; D027682:Cation Transport Proteins; D000079922:Copper Transport Proteins; D005656:Fungal Proteins; D018832:Molecular Chaperones; D011994:Recombinant Proteins; D029701:Saccharomyces cerevisiae Proteins; D003300:Copper