Effects of fibronectin fragments on DNA transfection into mammalian cells by electroporation.
Two kinds of cells, human epidermoid carcinoma A431 cells and simian kidney COS-7 cells, were transfected with the chloramphenicol acetyl transferase (CAT) gene by electroporation, and then cultivated on culture dishes coated with two different forms of recombinant fibronectin fragments consisting of cell-binding domain (C-274) or heparin-binding domain and CS1 region (H-296). In the case of A431 cells, H-296-coated dishes significantly increased the amount of expressed CAT and the adhesion of electroporated cells in comparison with non-coated dishes. C-274 was effective for COS-7 cells. Overall, these fibronectin fragments increased the recovery of the transfectants on A-431 cells and COS-7 cells, respectively.
Hashino
K
K
Biotechnology Research Laboratories, Takara Shuzo Co., Ltd., Otsu, Shiga.
Matsushita
H
H
Kato
I
I
D000818:Animals; D019556:COS Cells / Q000166:cytology / Q000502:physiology; D002294:Carcinoma, Squamous Cell / Q000235:genetics / Q000473:pathology; D002448:Cell Adhesion / Q000502:physiology; D002470:Cell Survival / Q000187:drug effects; D004247:DNA / Q000235:genetics*; D018274:Electroporation / Q000379:methods*; D005353:Fibronectins / Q000494:pharmacology*; D006801:Humans; D051379:Mice; D009101:Multiple Myeloma / Q000235:genetics / Q000473:pathology; D010446:Peptide Fragments / Q000494:pharmacology*; D014161:Transduction, Genetic / Q000187:drug effects*; D014162:Transfection / Q000379:methods*
D005353:Fibronectins; D010446:Peptide Fragments; D004247:DNA