PCR analysis of human telomeric repeats present on HHV-6A viral strains.
Human herpesvirus 6 (HHV-6) presents a perfect tandem array of human telomeric repeats (TRS) at both identical direct repeats (DR). Several researchers have reported a different TRS copy number by sequence analysis of HHV-6 DR's cloned fragments so it has been hypothesized that number of TRS is unstable. By PCR we show that the TRS copy number of U1102 HHV-6 variant A strains is stable during viral cultivation in cell lines and each HHV-6 variant A strain, detected in pathologic specimens, is characterized by a specific TRS copy number.
Mirandola
P
P
Institute of Microbiology, University of Ferrara, Italy.
Ravaioli
T
T
Cassai
E
E
D002460:Cell Line / Q000821:virology; D004279:DNA, Viral / Q000032:analysis / Q000302:isolation & purification; D014644:Genetic Variation; D015654:Herpesvirus 6, Human / Q000235:genetics*; D006801:Humans; D016133:Polymerase Chain Reaction; D012091:Repetitive Sequences, Nucleic Acid / Q000235:genetics* / Q000502:physiology; D012093:Replicon / Q000235:genetics / Q000502:physiology; D016615:Telomere / Q000235:genetics / Q000821:virology*; D014779:Virus Replication / Q000235:genetics
D004279:DNA, Viral