Rapid method for detection of point mutations using mismatch binding protein (MutS) and an optical biosensor.
This paper describes a new method for detecting DNA point mutations using a mismatch binding protein. The interactions of mismatches and mismatch binding proteins are detected by the optical biosensor technology based on surface plasmon resonance (SPR).
Gotoh
M
M
Research and Development Department, Pharmacia Biotech KK, Tokyo, Japan.
Hasebe
M
M
Ohira
T
T
Hasegawa
Y
Y
Shinohara
Y
Y
Sota
H
H
Nakao
J
J
Tosu
M
M
D000251:Adenosine Triphosphatases; D000255:Adenosine Triphosphate / Q000737:chemistry; D001426:Bacterial Proteins; D015374:Biosensing Techniques; D004252:DNA Mutational Analysis / Q000379:methods*; D004273:DNA, Neoplasm / Q000032:analysis*; D004268:DNA-Binding Proteins; D029968:Escherichia coli Proteins; D006801:Humans; D051719:MutS DNA Mismatch-Binding Protein; D017354:Point Mutation; D014407:Tumor Cells, Cultured
D001426:Bacterial Proteins; D004273:DNA, Neoplasm; D004268:DNA-Binding Proteins; D029968:Escherichia coli Proteins; D000255:Adenosine Triphosphate; D000251:Adenosine Triphosphatases; D051719:MutS DNA Mismatch-Binding Protein; C497175:MutS protein, E coli