title: The IPL gene on chromosome 11p15.5 is imprinted in humans and mice and is similar to TDAG51, implicated in Fas expression and apoptosis.
abstract: We searched for novel imprinted genes in a region of human chromosome 11p15.5, which contains several known imprinted genes. Here we describe the cloning and characterization of the IPL ( I mprinted in P lacenta and L iver) gene, which shows tissue-specific expression and functional imprinting, with the maternal allele active and the paternal allele relatively inactive, in many human and mouse tissues. Human IPL is highly expressed in placenta and shows low but detectable expression in fetal and adult liver and lung. Mouse Ipl maps to the region of chromosome 7 which is syntenic with human 11p15.5 and this gene is expressed in placenta and at higher levels in extraembryonic membranes (yolk sac), fetal liver and adult kidney. Mouse and human IPL show sequence similarity to TDAG51 , a gene which was shown to be essential for Fas expression and susceptibility to apoptosis in a T lymphocyte cell line. Like several other imprinted genes, mouse and human IPL genes are small and contain small introns. These data expand the repertoire of known imprinted genes and will be helpful in testing the mechanism of genomic imprinting and the role of imprinted genes in growth regulation.
authors.affiliation: Department of Pathology, Columbia University College of Physicians and Surgeons, New York, NY 10032, USA.
authors.forename: N
authors.initials: N
authors.lastname: Qian
authors.forename: D
authors.initials: D
authors.lastname: Frank
authors.forename: D
authors.initials: D
authors.lastname: O'Keefe
authors.forename: D
authors.initials: D
authors.lastname: Dao
authors.forename: L
authors.initials: L
authors.lastname: Zhao
authors.forename: L
authors.initials: L
authors.lastname: Yuan
authors.forename: Q
authors.initials: Q
authors.lastname: Wang
authors.forename: M
authors.initials: M
authors.lastname: Keating
authors.forename: C
authors.initials: C
authors.lastname: Walsh
authors.forename: B
authors.initials: B
authors.lastname: Tycko
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D017209:Apoptosis / Q000235:genetics*; D001483:Base Sequence; D002874:Chromosome Mapping; D002880:Chromosomes, Human, Pair 11 / Q000737:chemistry*; D003001:Cloning, Molecular; D005260:Female; D015870:Gene Expression; D018392:Genomic Imprinting; D006801:Humans; D008099:Liver / Q000378:metabolism*; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D008969:Molecular Sequence Data; D009687:Nuclear Proteins; D009928:Organ Specificity / Q000235:genetics; D010920:Placenta / Q000378:metabolism*; D011506:Proteins / Q000235:genetics* / Q000378:metabolism; D017422:Sequence Analysis, DNA; D019014:fas Receptor / Q000096:biosynthesis* / Q000235:genetics
chemical_list: D009687:Nuclear Proteins; D011506:Proteins; C109277:TSSC3 protein; D019014:fas Receptor