Isolation and characterization of Xenopus laevis xSox-B1 cDNA.
A family of genes related to the sex-determining region Y gene (SRY) has been termed SOX, and the members are known to encode transcriptional factors and be involved in animal development. We have isolated and sequenced a novel Sox cDNA of Xenopus laevis in this study. The cDNA encodes a protein of 309 amino acids, which encompasses a B-type SOX HMG box; the cDNA was named xSox-B1 in this study. The xSox-B1 mRNA is 1.6 kb in length. Various tissues of adult frog were tested for xSox-B1 mRNA by reverse transcription/ polymerase chain reaction, and the results showed that xSox-B1 expression was highly restricted to the ovary. The message of the xSox-B1 gene was also detected in unfertilized eggs and the late blastula embryos of X. laevis. Recombinant polypeptide of the xSox-B1 HMG domain preferentially binds to the AACAAT sequence.
Sakai
Y
Y
Department of Anatomy, Keio University School of Medicine, Tokyo, Japan.
Hiraoka
Y
Y
Konishi
M
M
Ogawa
M
M
Aiso
S
S
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D001665:Binding Sites; D018076:DNA, Complementary / Q000235:genetics; D005260:Female; D015870:Gene Expression; D015723:Gene Library; D008969:Molecular Sequence Data; D005810:Multigene Family; D010053:Ovary / Q000737:chemistry; D016133:Polymerase Chain Reaction; D011485:Protein Binding; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D014018:Tissue Distribution; D014982:Xenopus laevis / Q000235:genetics*
D018076:DNA, Complementary