title: Regulation of receptor binding affinity of influenza virus hemagglutinin by its carbohydrate moiety.
abstract: The hemagglutinin (HA) of the fowl plague virus (FPV) strain of influenza A virus has two N-linked oligosaccharides attached to Asn123 and Asn149 in the vicinity of the receptor binding site. The effect of these carbohydrate side chains on the binding of HA to neuraminic acid-containing receptors has been analyzed. When the oligosaccharides were deleted by site-specific mutagenesis, HA expressed from a simian virus 40 vector showed enhanced hemadsorbing activity. Binding was so strong under these conditions that erythrocytes were no longer released by viral neuraminidase and that release was significantly reduced when neuraminidase from Vibrio cholerae was used. Similarly, when these oligosaccharides were removed selectively from purified viruses by N-glycosidase F, such virions were unable to elute from receptors, although they retained neuraminidase activity. Thus, release of FPV from cell receptors depends on the presence of the HA glycans at Asn123 and Asn149. On the other hand, receptor binding was abolished when these oligosaccharides were sialylated after expression in the absence of neuraminidase (M. Ohuchi, A. Feldmann, R. Ohuchi, and H.-D. Klenk, Virology 212:77-83, 1995). These observations indicate that the receptor affinity of FPV HA is controlled by oligosaccharides adjacent to the receptor binding site.
authors.affiliation: Institut für Virologie, Philipps-Universität Marburg, Germany.
authors.forename: M
authors.initials: M
authors.lastname: Ohuchi
authors.forename: R
authors.initials: R
authors.lastname: Ohuchi
authors.forename: A
authors.initials: A
authors.lastname: Feldmann
authors.forename: H D
authors.initials: HD
authors.lastname: Klenk
mesh_terms: D000818:Animals; D001665:Binding Sites; D002460:Cell Line; D002478:Cells, Cultured; D004285:Dogs; D004912:Erythrocytes / Q000821:virology; D006031:Glycosylation; D019267:Hemagglutinin Glycoproteins, Influenza Virus / Q000737:chemistry* / Q000378:metabolism; D009980:Influenza A virus / Q000378:metabolism*; D009439:Neuraminidase / Q000737:chemistry* / Q000378:metabolism; D011991:Receptors, Virus / Q000378:metabolism*; D013329:Structure-Activity Relationship; D014771:Virion / Q000378:metabolism
chemical_list: D019267:Hemagglutinin Glycoproteins, Influenza Virus; D011991:Receptors, Virus; D009439:Neuraminidase