title: cDNA cloning of a human dishevelled DVL-3 gene, mapping to 3q27, and expression in human breast and colon carcinomas.
abstract: dishevelled (Dsh) is a member of the segment polarity gene family in Drosophila which plays an important role in the early developmental patterning processes. A human homologue of Dsh (DVL-1) has recently been described. Here, we report the cloning of a second human homologue of Dsh (called DVL-3) by cDNA library screening. The human DVL-3 gene encodes a predicted 716 amino acid protein which exhibits 98% amino acid identity with mouse Dvl-3 and 49% with Drosophila Dsh. DVL-3 was mapped to 3q27. The expression of DVL-3 mRNA was detected in 30 human cell lines and 2 primary cell cultures. DVL-3 mRNA was detected in normal human breast tissues (n = 4) and tumours (n = 25). Statistically, there was no difference in DVL-3 mRNA level between normal breast tissues and tumours. In human colorectal samples, DVL-3 was expressed equally in matched normal tissues, polyps and tumours. The data indicates that DVL-3 is widely expressed in human cells and supports the notion of a new developmental gene family for dishevelled which may have a widespread role in signal transduction.
authors.affiliation: Imperial Cancer Research Fund, University of Oxford, Headington, Oxford, United Kingdom.
authors.forename: T D
authors.initials: TD
authors.lastname: Bui
authors.forename: D R
authors.initials: DR
authors.lastname: Beier
authors.forename: M
authors.initials: M
authors.lastname: Jonssen
authors.forename: K
authors.initials: K
authors.lastname: Smith
authors.forename: S M
authors.initials: SM
authors.lastname: Dorrington
authors.forename: L
authors.initials: L
authors.lastname: Kaklamanis
authors.forename: L
authors.initials: L
authors.lastname: Kearney
authors.forename: R
authors.initials: R
authors.lastname: Regan
authors.forename: D J
authors.initials: DJ
authors.lastname: Sussman
authors.forename: A L
authors.initials: AL
authors.lastname: Harris
mesh_terms: D048868:Adaptor Proteins, Signal Transducing; D000595:Amino Acid Sequence; D001483:Base Sequence; D001940:Breast / Q000378:metabolism; D001943:Breast Neoplasms / Q000235:genetics*; D002277:Carcinoma / Q000235:genetics*; D002478:Cells, Cultured; D002874:Chromosome Mapping; D002893:Chromosomes, Human, Pair 3; D003001:Cloning, Molecular; D003106:Colon / Q000378:metabolism; D003110:Colonic Neoplasms / Q000235:genetics*; D003111:Colonic Polyps; D018076:DNA, Complementary / Q000302:isolation & purification*; D000072261:Dishevelled Proteins; D029721:Drosophila Proteins; D018507:Gene Expression Regulation, Developmental; D005796:Genes; D050437:Genes, Developmental; D006801:Humans; D008969:Molecular Sequence Data; D010750:Phosphoproteins; D014176:Protein Biosynthesis; D011506:Proteins / Q000235:genetics*; D014407:Tumor Cells, Cultured
chemical_list: D048868:Adaptor Proteins, Signal Transducing; D018076:DNA, Complementary; C000606578:DVL1 protein, human; C000606584:DVL3 protein, human; D000072261:Dishevelled Proteins; D029721:Drosophila Proteins; C000606579:Dvl1 protein, mouse; C000606585:Dvl3 protein, mouse; D010750:Phosphoproteins; D011506:Proteins; C000606588:dsh protein, Drosophila