Infection of macrophages by Theiler's murine encephalomyelitis virus is highly dependent on their activation or differentiation state.
Macrophages are the main targets of Theiler's murine encephalomyelitis virus (TMEV) during persistent demyelinating infection of mice. Replication of TMEV in macrophages was previously shown to depend on their activation state. Here, we show that the quality of the serum used for culture drastically influences viral entry in RAW264.7 macrophages.
Shaw-Jackson
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C
International Institute of Cellular and Molecular Pathology, University of Louvain, Brussels, Belgium.
Michiels
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T
D000818:Animals; D002454:Cell Differentiation; D002460:Cell Line; D003470:Culture Media; D003588:Cytopathogenic Effect, Viral; D008262:Macrophage Activation; D008264:Macrophages / Q000166:cytology / Q000821:virology*; D051379:Mice; D011051:Poliomyelitis / Q000473:pathology; D017945:Theilovirus / Q000254:growth & development*; D014409:Tumor Necrosis Factor-alpha / Q000096:biosynthesis
D003470:Culture Media; D014409:Tumor Necrosis Factor-alpha