A Penicillium freii gene that is highly similar to the beta-ketoacyl synthase domain of polyketide synthase genes from other fungi.
A Penicillium freii DNA fragment with similarity to the beta-keto-acyl synthase motif of the P. griseofulvum 6-methylsalicylic acid synthase encoding gene (MSAS) was identified by screening a cosmid library using a part of MSAS as the probe. Two exons of 93 and 849 bp, encoding a predicted polypeptide of 314 amino acids, and a molecular mass of 33.4 kDa, were identified (PfKS). PfKS was transcribed as a 1.6 kbp messenger. A region corresponding to the MSAS gene encoding essential enzyme activities for the assembly of a polyketide chain was absent in PfKS. Gene disruption experiments in P. freii with a truncated version of PfKS did not result in the detection of homologous integration events.
Nicolaisen
M
M
Biotechnological Institute, Technical University of Denmark, Lyngby, Denmark. m.nicolaisen@dips-lyngby.dk
Frisvad
J C
JC
Rossen
L
L
D001483:Base Sequence; D003001:Cloning, Molecular; D017931:DNA Primers / Q000235:genetics; D005658:Fungi / Q000201:enzymology / Q000235:genetics; D005800:Genes, Fungal; D009097:Multienzyme Complexes / Q000737:chemistry / Q000235:genetics*; D010407:Penicillium / Q000201:enzymology* / Q000235:genetics*; D016133:Polymerase Chain Reaction; D014158:Transcription, Genetic; D014170:Transformation, Genetic
D017931:DNA Primers; D009097:Multienzyme Complexes