Anti-Thy-1 antibody provokes reverse passive cutaneous anaphylaxis-like reactions.
Antibodies to rat Thy-1, and also to murine Thy-1, could provoke reverse passive cutaneous anaphylaxis-like (RPCA-like) reactions. The threshold amount of purified anti-Thy-1 antibodies for RPCA-like reactions was 12.5 microg/ml. No RPCA-like reactions were obtained with IgG1 F(ab')2 anti-Thy-1. Cross-linking the murine anti-rat Thy-1 F(ab')2 fragments with goat antibody against murine F(ab')2 anti-rat Thy-1 permitted us to obtain RPCA-like reactions with as little as 1.5 microg/ ml. However, when the cross-linking was done with the F(ab')2 of the same goat antibody, no RPCA-like reactions were obtained. The antibody against rat FcepsilonRIalpha or its F(ab')2 product were equally effective in producing RPCA-like reactions; the threshold was obtained with 1.5 microg/ml. The mechanism of the difference is discussed.
Yamaji
C
C
Department of Internal Medicine, School of Medicine, Juntendo University, Tokyo, Japan.
Hirano
T
T
Miyajima
H
H
Hashimoto
H
H
Okumura
K
K
Ovary
Z
Z
D000818:Animals; D000911:Antibodies, Monoclonal; D015550:Cell Degranulation; D005455:Fluorescent Antibody Technique; D007140:Immunoglobulin Fab Fragments; D008297:Male; D008407:Mast Cells / Q000276:immunology*; D010323:Passive Cutaneous Anaphylaxis / Q000276:immunology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D017455:Receptors, IgE / Q000032:analysis; D018800:Thy-1 Antigens / Q000032:analysis / Q000276:immunology*; D001619:beta-N-Acetylhexosaminidases / Q000378:metabolism
D000911:Antibodies, Monoclonal; D007140:Immunoglobulin Fab Fragments; D017455:Receptors, IgE; D018800:Thy-1 Antigens; D001619:beta-N-Acetylhexosaminidases