title: Vaginal carriage of enterotoxigenic Bacteroides fragilis in pregnant women.
abstract: Bacteroides fragilis is an anaerobic bacterial species that is involved in gynecological infections and pathology. The incidence of vaginal carriage is largely unknown, and in order to study this, 120 pregnant women attending a general hospital for delivery were examined. Cultures were positive for eight of these women (6.6%). Interestingly, potential clonal relatedness could be demonstrated among several of the nonenterotoxigenic B. fragilis strains. Among the strains, only one produced metalloprotease enterotoxin. The presence of the gene for the metalloprotease, giving rise to the pathogenic effect on cultured eukaryotic HT29/C1 cells, was confirmed by a newly designed specific PCR assay. The enterotoxigenic B. fragilis (ETBF) strain was analyzed with the help of arbitrarily primed PCR (AP-PCR) and PCR-mediated ribotyping. The ETBF strain was shown to be genetically different compared to several other strains obtained from diverse sources. Our data indicate a relatively high vaginal B. fragilis carriage rate among pregnant women in Warsaw, Poland. Although neither ETBF nor B. fragilis colonization presented a clinical problem, the possible genetic relatedness among the colonizing B. fragilis strains indicates the need for additional research in the field of ETBF transmission and molecular epidemiology.
authors.affiliation: Department of Clinical Bacteriology, Warsaw Medical Academy, Poland.
authors.forename: P
authors.initials: P
authors.lastname: Leszczynski
authors.forename: A
authors.initials: A
authors.lastname: van Belkum
authors.forename: H
authors.initials: H
authors.lastname: Pituch
authors.forename: H
authors.initials: H
authors.lastname: Verbrugh
authors.forename: F
authors.initials: F
authors.lastname: Meisel-Mikolajczyk
mesh_terms: D001442:Bacteroides Infections / Q000175:diagnosis*; D001441:Bacteroides fragilis / Q000235:genetics / Q000302:isolation & purification* / Q000472:pathogenicity; D017931:DNA Primers; D004269:DNA, Bacterial / Q000032:analysis; D004768:Enterotoxins / Q000235:genetics*; D005260:Female; D006801:Humans; D016133:Polymerase Chain Reaction / Q000379:methods; D011247:Pregnancy; D011251:Pregnancy Complications, Infectious / Q000175:diagnosis* / Q000382:microbiology; D014621:Vagina / Q000382:microbiology*; D014626:Vaginal Smears
chemical_list: D017931:DNA Primers; D004269:DNA, Bacterial; D004768:Enterotoxins