title: Properties of hepatitis delta virus ribozyme, which consists of three RNA oligomer strands.
abstract: Properties of a hepatitis delta virus (HDV) RNA ribozyme system, which consists of three RNA oligomer strands (substrate 8-mer; enzyme 16-mer plus 35-mer) and contains a hybrid sequence of genomic and antigenomic RNA cores, are reported. Effects of Mg2+ concentration, divalent metal ion species, pH, and temperature on the cleavage activity were examined. The substrate cleavage activity increased with increasing Mg2+ concentration (0-100 mM). Ca2+ and Mn2+ ions were the most effective divalent cations and Mg2+ was less effective. The cleavage activity increased with increasing pH (5-7.5). The optimum temperature for the cleavage activity was 25-40 degrees C. The Mg2+ concentration, pH and temperature dependencies are different from those reported for the single-strand ribozymes (about 90-mer) although the divalent metal ion preference is very similar. Conformational change induced by Mg2+ ion titration was monitored by CD. The CD data and the activity-Mg2+ concentration data were analyzed by curve-fitting analysis using equations derived for multiple metal ion binding mechanisms. The data can be explained by a model in which three Mg2+ ions bind to one ribozyme unit.
authors.affiliation: Department of Bioengineering, Faculty of Engineering, Yokohama National University.
authors.forename: T
authors.initials: T
authors.lastname: Sakamoto
authors.forename: Y
authors.initials: Y
authors.lastname: Tanaka
authors.forename: T
authors.initials: T
authors.lastname: Kuwabara
authors.forename: M H
authors.initials: MH
authors.lastname: Kim
authors.forename: Y
authors.initials: Y
authors.lastname: Kurihara
authors.forename: M
authors.initials: M
authors.lastname: Katahira
authors.forename: S
authors.initials: S
authors.lastname: Uesugi
mesh_terms: D001483:Base Sequence; D001704:Biopolymers; D003698:Hepatitis Delta Virus / Q000201:enzymology / Q000235:genetics*; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation; D016337:RNA, Catalytic / Q000032:analysis*; D012367:RNA, Viral / Q000235:genetics*; D013696:Temperature
chemical_list: D001704:Biopolymers; D016337:RNA, Catalytic; D012367:RNA, Viral