Inhibition of DNA synthesis and tube morphogenesis of cultured vascular endothelial cells by chondromodulin-I.
Cartilage is an avascular tissue, and exhibits anti-angiogenic properties. Cartilage extracts have been shown to contain an inhibitor for DNA synthesis in vascular endothelial cells in vitro. Here we purified the inhibitory activity in the 10-50 kDa fraction of guanidine extracts from fetal bovine epiphyseal cartilage, and found that the inhibitor was identical with chondromodulin-I (ChM-I). Purified ChM-I inhibited tube morphogenesis of cultured vascular endothelial cells, as well as DNA synthesis. These results indicate that cartilage-specific glycoprotein ChM-I may participate in the maintenance of avascularity and anti-angiogenic properties of cartilage.
Hiraki
Y
Y
Department of Biochemistry, Osaka University Faculty of Dentistry, Suita, Japan.
Kono
T
T
Sato
M
M
Shukunami
C
C
Kondo
J
J
D000818:Animals; D002339:Carotid Arteries / Q000166:cytology; D002417:Cattle; D048429:Cell Size / Q000187:drug effects; D002478:Cells, Cultured; D002846:Chromatography, Affinity; D002850:Chromatography, Gel; D004247:DNA / Q000096:biosynthesis*; D004305:Dose-Response Relationship, Drug; D004730:Endothelium, Vascular / Q000166:cytology / Q000187:drug effects*; D006023:Glycoproteins / Q000378:metabolism; D006132:Growth Plate / Q000737:chemistry*; D006133:Growth Substances / Q000302:isolation & purification / Q000378:metabolism / Q000494:pharmacology*; D036341:Intercellular Signaling Peptides and Proteins; D009024:Morphogenesis / Q000187:drug effects; D018919:Neovascularization, Physiologic / Q000187:drug effects; D019384:Nucleic Acid Synthesis Inhibitors / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D013936:Thymidine / Q000378:metabolism
D006023:Glycoproteins; D006133:Growth Substances; D036341:Intercellular Signaling Peptides and Proteins; D019384:Nucleic Acid Synthesis Inhibitors; D004247:DNA; D013936:Thymidine