title: No salting-in of lysozyme chloride observed at low ionic strength over a large range of pH.
abstract: Solubility of lysozyme chloride was determined in the absence of added salt and in the presence of 0.05-1.2 M NaCl, starting from isoionic lysozyme, which was then brought to pH values from 9 to 3 by addition of HCl. The main observation is the absence of a salting-in region whatever the pH studied. This is explained by a predominant electrostatic screening of the positively charged protein and/or by adsorption of chloride ions by the protein. The solubility increases with the protein net charge at low ionic strength, but the reverse is observed at high ionic strength. The solubility of lysozyme chloride seems to become independent of ionic strength at pH approximately 9.5, which is interpreted as a shift of the isoionic pH (10.8) to an isoelectric pH due to chloride binding. The crystallization at very low ionic strength, where lysozyme crystallizes at supersaturation values as low as 1.1, amplifies the effect of pH on protein solubility. Understanding the effect of the net charge and of ionic strength on protein-protein interactions is valuable not only for protein crystal growth but more generally also for the formation of protein-protein or protein-ligand complexes.
authors.affiliation: Laboratoire d'Enzymologie et de Biochimie Structurales, CNRS, Gif sur Yvette, France.
authors.forename: P
authors.initials: P
authors.lastname: Retailleau
authors.forename: M
authors.initials: M
authors.lastname: Riès-Kautt
authors.forename: A
authors.initials: A
authors.lastname: Ducruix
mesh_terms: D000327:Adsorption; D000818:Animals; D055592:Biophysical Phenomena; D001703:Biophysics; D002645:Chickens; D002712:Chlorides / Q000737:chemistry; D003460:Crystallization; D006863:Hydrogen-Ion Concentration; D066298:In Vitro Techniques; D009113:Muramidase / Q000737:chemistry*; D009994:Osmolar Concentration; D011485:Protein Binding; D012965:Sodium Chloride; D012995:Solubility; D055672:Static Electricity; D013816:Thermodynamics
chemical_list: D002712:Chlorides; D012965:Sodium Chloride; D009113:Muramidase