title: Rapid PCR amplification of chimeric products and its direct application to in vivo [corrected] testing of recombinant DNA construction strategies.
abstract: This article describes a simple and rapid method for efficient production of chimeric products by polymerase chain reaction (PCR). This protocol is amenable to site-directed mutagenesis strategies and can be done without the time-consuming gel purification step. The PCR products generated can also be directly used for direct gene transfer into plant cells without further subcloning to test construction strategies.
authors.affiliation: Department of Plant and Microbial Biology, University of California, Berkeley 94720, USA. mjcho@nature.berkely.edu
authors.forename: M J
authors.initials: MJ
authors.lastname: Cho
authors.forename: P G
authors.initials: PG
authors.lastname: Lemaux
mesh_terms: D001483:Base Sequence; D017931:DNA Primers; D004274:DNA, Recombinant / Q000235:genetics*; D016297:Mutagenesis, Site-Directed; D016133:Polymerase Chain Reaction / Q000379:methods*; D011993:Recombinant Fusion Proteins / Q000235:genetics*
chemical_list: D017931:DNA Primers; D004274:DNA, Recombinant; D011993:Recombinant Fusion Proteins