Characterization of alpha-conotoxin interactions with the nicotinic acetylcholine receptor and monoclonal antibodies.
The venoms of predatory marine cone snails, Conus species, contain numerous peptides and proteins with remarkably diverse pharmacological properties. One group of peptides are the alpha-conotoxins, which consist of 13-19 amino acids constrained by two disulphide bonds. A biologically active fluorescein derivative of Conus geographus alpha-conotoxin GI (FGI) was used in novel solution-phase-binding assays with purified Torpedo californica nicotinic acetylcholine receptor (nAchR) and monoclonal antibodies developed against the toxin. The binding of FGI to nAchR or antibody had apparent dissociation constants of 10-100 nM. Structure-function studies with alpha-conotoxin GI analogues composed of a single disulphide loop revealed that different conformational restraints are necessary for effective toxin interactions with nAchR or antibodies.
Ashcom
J D
JD
Toxinology Division, USAMRIID, Fort Detrick, MD 21702-5011, USA.
Stiles
B G
BG
D000595:Amino Acid Sequence; D000818:Animals; D000911:Antibodies, Monoclonal / Q000378:metabolism* / Q000494:pharmacology; D001666:Binding Sites, Antibody; D002498:Centrifugation; D002850:Chromatography, Gel; D020916:Conotoxins; D005452:Fluoresceins / Q000378:metabolism; D007928:Lethal Dose 50; D051379:Mice; D008969:Molecular Sequence Data; D008978:Mollusk Venoms / Q000276:immunology / Q000378:metabolism*; D010456:Peptides, Cyclic / Q000276:immunology / Q000378:metabolism*; D011485:Protein Binding; D011978:Receptors, Nicotinic / Q000378:metabolism*; D012996:Solutions; D013050:Spectrometry, Fluorescence; D014101:Torpedo
D000911:Antibodies, Monoclonal; D020916:Conotoxins; D005452:Fluoresceins; D008978:Mollusk Venoms; D010456:Peptides, Cyclic; D011978:Receptors, Nicotinic; D012996:Solutions; C029960:conotoxin GI