title: A two year prospective study to compare culture and polymerase chain reaction amplification for the detection and diagnosis of Lyme borreliosis.
abstract: OBJECTIVE
  To compare polymerase chain reaction (PCR) amplification of borrelial DNA and culture isolation of spirochaetes for the diagnosis of Lyme borreliosis by direct detection of Borrelia burgdorferi sensu lato in patients with erythema migrans and acrodermatitis chronica atrophicans lesions.
  
  
  METHODS
  Skin biopsy specimens from erythema migrans and acrodermatitis chronica atrophicans lesions were subdivided and tested by PCR amplification assay and culture using two artificial growth media, Barbour-Stoenner-Kelly II (BSK II) and modified Kelly-Pettenkofer (MKP). Five classes of lesions were studied: typical erythema migrans, spontaneously resolved erythema migrans, atypical/partially treated erythema migrans, typical acrodermatitis chronica atrophicans, and atypical/partially treated acrodermatitis chronica atrophicans.
  
  
  RESULTS
  For both erythema migrans and acrodermatitis chronica atrophicans lesions, the most sensitive detection method was MKP culture. PCR was less sensitive than MKP culture, but more sensitive than BSK II culture. Results for 758 typical erythema migrans specimens showed positivity rates of 36% for MKP, 25% for PCR, and 24% for BSK II. Differences were statistically significant. The overall positivity rate for all three methods combined was 54%, but few specimens (6%) were positive by all three methods. Examination of multiple erythema migrans lesions from the same patient increased the diagnostic yield. These findings, and similar results for acrodermatitis chronica atrophicans lesions, suggest that the distribution of spirochaetes in skin biopsies is not homogeneous.
  
  
  CONCLUSIONS
  Although possessing the potential to provide a rapid diagnosis, PCR is not more sensitive than culture for the direct detection of borrelia. Spirochaetes appear to be unevenly distributed throughout biopsy specimens, suggesting that diagnosis of Lyme borreliosis by direct detection of the causative agent in skin lesions in vulnerable to sample bias.
authors.affiliation: Department of Pathology, Loyola University Medical Center, Maywood, IL, USA.
authors.forename: M M
authors.initials: MM
authors.lastname: Picken
authors.forename: R N
authors.initials: RN
authors.lastname: Picken
authors.forename: D
authors.initials: D
authors.lastname: Han
authors.forename: Y
authors.initials: Y
authors.lastname: Cheng
authors.forename: E
authors.initials: E
authors.lastname: Ruzic-Sabljic
authors.forename: J
authors.initials: J
authors.lastname: Cimperman
authors.forename: V
authors.initials: V
authors.lastname: Maraspin
authors.forename: S
authors.initials: S
authors.lastname: Lotric-Furlan
authors.forename: F
authors.initials: F
authors.lastname: Strle
mesh_terms: D000169:Acrodermatitis / Q000175:diagnosis / Q000382:microbiology; D001431:Bacteriological Techniques; D015748:Borrelia burgdorferi Group / Q000302:isolation & purification*; D003470:Culture Media; D004269:DNA, Bacterial / Q000032:analysis; D015787:Erythema Chronicum Migrans / Q000175:diagnosis; D006801:Humans; D008193:Lyme Disease / Q000175:diagnosis*; D016133:Polymerase Chain Reaction / Q000379:methods*; D011446:Prospective Studies; D012867:Skin / Q000382:microbiology
chemical_list: D003470:Culture Media; D004269:DNA, Bacterial