Reticalmin: a novel calcium/calmodulin-dependent protein kinase IV-like protein in rat retina.
Western blot analysis of 100,000 g supernatant of rat retina using a polyclonal anti-Ca2+/ calmodulin-dependent protein kinase IV (CaM-kinase IV) antibody revealed an immunoreactive mass of 35 kDa, termed reticalmin. Lower amount of a isoform of CaM-kinase IV was also expressed in rat retina. Reticalmin did not react with anti-CaM-kinase IV C-terminal peptide antibody which recognized alpha and beta isoforms of CaM-kinase IV and calspermin. Immunohistochemically reticalmin was shown to be localized mainly in the outer segment of photo-receptor cells, and in dendrites of inner plexiform layers and may be in nuclei of ganglion cells and some inner nuclear layer cells.
Hirooka
K
K
Department of Physiology, Kagawa Medical University, Japan.
Tokuda
M
M
Tsumura
T
T
Ahmed
B Y
BY
Itano
T
T
Matsui
H
H
Konishi
R
R
Hasegawa
E
E
Okuno
S
S
Kitani
T
T
Fujisawa
H
H
Hatase
O
O
D000818:Animals; D015153:Blotting, Western; D017871:Calcium-Calmodulin-Dependent Protein Kinases / Q000032:analysis*; D005136:Eye Proteins / Q000032:analysis*; D007150:Immunohistochemistry; D008297:Male; D008970:Molecular Weight; D051381:Rats; D017207:Rats, Sprague-Dawley; D012160:Retina / Q000737:chemistry*
D005136:Eye Proteins; C108488:reticalmin; D017871:Calcium-Calmodulin-Dependent Protein Kinases