Yeast exoglycoproteins produced under NaCl-stress conditions as efficient cryoprotective agents.
Six extracellular yeast glycoproteins were prepared from three yeast species in osmotic equilibrium and unequilibrium environments and used as non-penetrating cryoadditives. The glycoprotein secreted by the strain Dipodascus australiensis into the growth medium containing NaCl (8% w/v) was found to be the most effective cryoadditive. It was possible to use this glycoprotein alone (without penetrating agent DMSO) for the cryoprotection of the yeasts studied.
Breierová
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Institute of Chemistry, Slovak Academy of Sciences, Dúbravská, Bratislava, Slovakia. chememi@savba.sk
D002175:Candida / Q000378:metabolism; D003451:Cryoprotective Agents / Q000302:isolation & purification / Q000378:metabolism* / Q000494:pharmacology; D005615:Freezing; D005656:Fungal Proteins / Q000096:biosynthesis* / Q000302:isolation & purification / Q000494:pharmacology; D006023:Glycoproteins / Q000096:biosynthesis* / Q000302:isolation & purification / Q000494:pharmacology; D004718:Saccharomycetales / Q000378:metabolism; D012965:Sodium Chloride; D015003:Yeasts / Q000145:classification / Q000187:drug effects / Q000378:metabolism*
D003451:Cryoprotective Agents; D005656:Fungal Proteins; D006023:Glycoproteins; D012965:Sodium Chloride