Sequence analysis demonstrates that VP6, NSP1 and NSP4 genes of Indian neonatal rotavirus strain 116E are of human origin.
We have sequenced the genes encoding the inner capsid protein VP6 and the nonstructural proteins NSP1 and NSP4 of the Indian neonatal serotype P8[11]G9 human/bovine reassortant candidate vaccine rotavirus strain 116E. These three genes share a high degree of sequence and deduced amino acid homology with human prototype strain Wa. Our results confirm and extend those of previous RNA-RNA hybridization studies which suggested that these genes are of human origin, and will facilitate examination of the host immune response to 116E induced by natural infection and vaccination.
Cunliffe
N A
NA
Division of Viral and Rickettsial Diseases, National Center for Infectious Diseases, Centers for Disease Control and Prevention, Department of Health and Human Services, Atlanta, Georgia, USA.
Das
B K
BK
Ramachandran
M
M
Bhan
M K
MK
Glass
R I
RI
Gentsch
J R
JR
D000595:Amino Acid Sequence; D000956:Antigens, Viral; D002213:Capsid / Q000235:genetics*; D036022:Capsid Proteins; D017124:Conserved Sequence; D005814:Genes, Viral; D006023:Glycoproteins / Q000235:genetics*; D006801:Humans; D007194:India; D007231:Infant, Newborn; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D012401:Rotavirus / Q000737:chemistry / Q000145:classification / Q000235:genetics*; D017421:Sequence Analysis; D017386:Sequence Homology, Amino Acid; D014118:Toxins, Biological; D017361:Viral Nonstructural Proteins / Q000235:genetics*; D015678:Viral Structural Proteins / Q000235:genetics*
D000956:Antigens, Viral; D036022:Capsid Proteins; D006023:Glycoproteins; C081369:NS28 protein, rotavirus; D014118:Toxins, Biological; C061332:VP6 protein, Rotavirus; D017361:Viral Nonstructural Proteins; D015678:Viral Structural Proteins; C104282:nsp1 protein, Rotavirus