Bacteriocin typing of Klebsiella spp. isolated from different sources.
To evaluate whether clinical Klebsiella isolates differ from nonclinical strains with respect to bacteriocin susceptibility patterns, a total of 452 Klebsiella pneumoniae and K. oxytoca strains isolated from different sources were examined. Bacteriocin typing was done by a modification of the scrape-and-point method, using a set of eight producer strains. 96% of the strains were typable. Forty-one different bacteriocin susceptibility patterns were observed. While two thirds of the K. oxytoca isolates belonged to only three different bacteriocin types, the K. pneumoniae strains showed a more heterogeneous distribution of patterns. No differences in pattern distribution were observed between isolates from clinical, fecal, or environmental sources. Certain bacteriocins showed a very broad spectrum of activity; e.g. 93% of all isolates were susceptible to bacteriocin type 3. The results suggest that nonclinical Klebsiella strains do not show other bacteriocin susceptibility types than clinical isolates do.
Podschun
R
R
Institut für Medizinische Mikrobiologie und Virologie, Universität Kiel.
Ullmann
U
U
D001430:Bacteriocins; D001431:Bacteriological Techniques; D003470:Culture Media; D005243:Feces / Q000382:microbiology; D006801:Humans; D007709:Klebsiella / Q000145:classification* / Q000302:isolation & purification; D007711:Klebsiella pneumoniae / Q000145:classification / Q000302:isolation & purification; D012703:Serotyping; D012722:Sewage
D001430:Bacteriocins; D003470:Culture Media; D012722:Sewage