Direct cloning of the Brassica S locus by using a P1-derived artificial chromosome (PAC) vector.
Self-incompatibility of Brassica is regulated by the S locus, which contains several genes including SLG and SRK. We found that both SLG and SRK genes were located at an approx. 80-kb MluI fragment in an S9 haplotype of B. campestris. Therefore, we cloned this MluI fragment into a BssHII site of the P1-derived artificial chromosome (PAC) vector. The utility of the direct cloning method is discussed in this study.
Suzuki
G
G
Laboratory of Plant Breeding, Faculty of Agriculture, Tohoku University, Sendai, Japan.
Watanabe
M
M
Toriyama
K
K
Isogai
A
A
Hinata
K
K
D001419:Bacteria / Q000235:genetics; D001426:Bacterial Proteins; D001937:Brassica / Q000235:genetics*; D003001:Cloning, Molecular / Q000379:methods*; D015252:Deoxyribonucleases, Type II Site-Specific; D017343:Genes, Plant / Q000235:genetics*; D005822:Genetic Vectors / Q000235:genetics*; D006023:Glycoproteins / Q000235:genetics*; D010940:Plant Proteins / Q000235:genetics*; D010957:Plasmids / Q000235:genetics; D011494:Protein Kinases / Q000235:genetics
D001426:Bacterial Proteins; D006023:Glycoproteins; D010940:Plant Proteins; C064156:self-incompatibility locus specific glycoprotein, Brassica; D011494:Protein Kinases; C079832:S-receptor kinase; C035041:endodeoxyribonuclease MluI; D015252:Deoxyribonucleases, Type II Site-Specific