title: A developmental expression of AMPA-selective glutamate receptor subunits in human basal ganglia.
abstract: The development of AMPA-selective glutamate receptors (GluR1 and GluR2-3) in human basal ganglia (BG) was investigated in 23 normal brains by means of an immunohistochemical method. Immunoreactivity to GluR1 and 2-3 was detected in the cytoplasm and dendrites of small and large neurons in the BG. GluR2-3 immunoreactivity-positive neurons were clearly observed at 23-24 gestational weeks (GW) in the globus pallidus and at 32 GW in the neostriatum, and reached a peak at 32 GW and 39 GW, respectively. GluR2-3 positive neurons in the BG began to decrease at 1-4 months of age, reaching the low level of adults by 7 months of age. The developmental pattern of GluR1 was similar to that of GluR2-3 in the BG, but the immunoreactivity to GluR1 was a little weaker than that of GluR2-3 in the neostriatum. Furthermore, GluR1 and 2-3 subunit Western blotting confirmed the specificity of the immunohistochemistry. Our results suggest that the development of GluR1 and 2-3 in the BG is consistent with neuronal development in the BG, which supports further that GluR1 and 2-3 play an important role in neuronal differentiation and maturation of the BG.
authors.affiliation: Department of Mental Retardation and Birth Defect Research, National Institute of Neuroscience, Tokyo, Japan.
authors.forename: S Z
authors.initials: SZ
authors.lastname: Meng
authors.forename: T
authors.initials: T
authors.lastname: Obonai
authors.forename: H
authors.initials: H
authors.lastname: Isumi
authors.forename: S
authors.initials: S
authors.lastname: Takashima
mesh_terms: D000293:Adolescent; D000328:Adult; D001479:Basal Ganglia / Q000254:growth & development* / Q000378:metabolism*; D015153:Blotting, Western; D002648:Child; D002675:Child, Preschool; D018846:Excitatory Amino Acids / Q000633:toxicity; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D007223:Infant; D007231:Infant, Newborn; D008875:Middle Aged; D011247:Pregnancy; D018091:Receptors, AMPA / Q000096:biosynthesis*
chemical_list: D018846:Excitatory Amino Acids; D018091:Receptors, AMPA