title: Determination of disodium cromoglycate in human urine by high-performance liquid chromatography with post-column photoirradiation-fluorescence detection.
abstract: For the determination of disodium cromoglycate in urine, a fluorimetric method using HPLC post-column photoirradiation has been developed. The mobile phase consisted of a 35 mmol l-1 phosphate buffer (pH 8)-methanol (7 + 3, %v/v) containing 75 mmol l-1 hydrogen peroxide and 20 mmol l-1 18-crown-6. The 18-crown-6 was used for separation adjustment of the disodium cromoglycate in the urine sample. Photoirradiation was carried out in tubing wound around a germicidal light in a reactor equipped with an air-cooling fan. The fluorescence was monitored with excitation at 325 nm and emission at 448 nm. The calibration graph for disodium cromoglycate was linear over the range 38-2340 ng ml-1 using an injection volume of 100 microliters. The pretreatment of the urine samples consisted of diluting and filtering steps. The mean recovery of disodium cromoglycate from urine was 99.1 +/- 2.4% (n = 6).
authors.affiliation: Department of Analytical Chemistry, Faculty of Pharmacutical Sciences, Teikyo University, Kanagawa, Japan.
authors.forename: K
authors.initials: K
authors.lastname: Mawatari
authors.forename: S
authors.initials: S
authors.lastname: Mashiko
authors.forename: Y
authors.initials: Y
authors.lastname: Sate
authors.forename: Y
authors.initials: Y
authors.lastname: Usui
authors.forename: F
authors.initials: F
authors.lastname: Iinuma
authors.forename: M
authors.initials: M
authors.lastname: Watanabe
mesh_terms: D000280:Administration, Inhalation; D018927:Anti-Asthmatic Agents / Q000008:administration & dosage / Q000652:urine*; D002851:Chromatography, High Pressure Liquid; D004205:Cromolyn Sodium / Q000008:administration & dosage / Q000652:urine*; D005470:Fluorometry; D006801:Humans; D008297:Male
chemical_list: D018927:Anti-Asthmatic Agents; D004205:Cromolyn Sodium