Gradient, high-resolution, magic-angle spinning nuclear magnetic resonance spectroscopy of human adipocyte tissue.
The recently developed technique of gradient, high-resolution magic-angle spinning NMR (g-hr-MAS-NMR) spectroscopy was applied to the study of ex vivo human lipoma and liposarcoma tissue. Compared with conventional 1H-NMR, the g-hr-MAS method yielded a large improvement in spectral resolution and permitted the detection of metabolite resonance's in a well-differentiated liposarcoma that was not observed in spectra of similar samples obtained using nonspinning NMR methods. These findings suggest that g-hr-MAS-NMR spectroscopy provides a key improvement in spectral quality for ex vivo lipoma and liposarcoma tissue thereby permitting a more precise determination of tissue metabolite composition than conventional nonspinning NMR methods.
Millis
K K
KK
Department of Surgery, Brigham and Women's Hospital, Harvard Medical School, Boston, Massachusetts, USA.
Maas
W E
WE
Cory
D G
DG
Singer
S
S
D017667:Adipocytes / Q000737:chemistry*; D006801:Humans; D007091:Image Processing, Computer-Assisted; D008055:Lipids / Q000032:analysis*; D008067:Lipoma / Q000737:chemistry; D008080:Liposarcoma / Q000737:chemistry; D009682:Magnetic Resonance Spectroscopy / Q000379:methods*; D008280:Magnetics; D018205:Neoplasms, Adipose Tissue / Q000737:chemistry*
D008055:Lipids