title: Trypanosoma rangeli: increase in virulence with inocula of different origins in the experimental infection in mice.
abstract: We compared two murine models of Trypanosoma rangeli infection. The same inoculum dose and age-matched hosts were used in both cases. One group was infected with trypomastigotes obtained from passages in mice and the other, with trypomastigotes obtained from cell culture after a passage in mice. We observed that trypomastigotes obtained from the in vitro cellular infection showed increased virulence in experimental animals, with a 70% rate of death being noted in experimental mice instead of the lack of mortality seen when in vivo-derived parasites were used. The greatest levels of parasitemia and tissual lesions in the presence of the parasite also occurred when in vitro-derived parasites were used.
authors.affiliation: Department of Preventive Medicine and Public Health, Autonomous University School of Medicine, Madrid, Spain
authors.forename: C
authors.initials: C
authors.lastname: Zuñiga
authors.forename: M T
authors.initials: MT
authors.lastname: Palau
authors.forename: P
authors.initials: P
authors.lastname: Penin
authors.forename: C
authors.initials: C
authors.lastname: Gamallo
authors.forename: J A
authors.initials: JA
authors.lastname: de Diego
mesh_terms: D000818:Animals; D002522:Chlorocebus aethiops; D008297:Male; D051379:Mice; D014345:Trypanosoma / Q000254:growth & development / Q000302:isolation & purification / Q000472:pathogenicity*; D014352:Trypanosomiasis / Q000469:parasitology; D014709:Vero Cells; D014774:Virulence