title: Adenosine A3 receptor stimulation inhibits migration of human eosinophils.
abstract: Activation of adenosine A3 receptors (A3-R) produced a dose-dependent reduction in the chemotaxis of human eosinophils to platelet-activating factor (PAF), RANTES, and leukotriene B4 (LTB4) to a maximum of 58, 48, and 52%, respectively (P < 0.02). This effect was completely reversed by selective A3-R antagonists. In contrast, activation of A1 or A2a-R did not affect PAF-induced eosinophil chemotaxis. PAF up-regulated the expression of CDllb/CD18, down-regulated L-selectin, and also increased F-actin assembly in eosinophils. The expression of these activation markers was not influenced by A3-R, A2a, or A1-R stimulation. Activation of A3-R may play an important role in inflammation by inhibiting eosinophil migration.
authors.affiliation: University of British Columbia Pulmonary Research Laboratory, St. Paul's Hospital, Vancouver, Canada.
authors.forename: D
authors.initials: D
authors.lastname: Knight
authors.forename: X
authors.initials: X
authors.lastname: Zheng
authors.forename: C
authors.initials: C
authors.lastname: Rocchini
authors.forename: M
authors.initials: M
authors.lastname: Jacobson
authors.forename: T
authors.initials: T
authors.lastname: Bai
authors.forename: B
authors.initials: B
authors.lastname: Walker
mesh_terms: D000241:Adenosine / Q000031:analogs & derivatives* / Q000494:pharmacology*; D018821:CD18 Antigens / Q000096:biosynthesis; D018946:Chemokine CCL5 / Q000494:pharmacology; D002634:Chemotaxis, Leukocyte / Q000187:drug effects*; D004804:Eosinophils / Q000187:drug effects / Q000502:physiology*; D006801:Humans; D066298:In Vitro Techniques; D019041:L-Selectin / Q000096:biosynthesis; D007975:Leukotriene B4 / Q000494:pharmacology; D016177:Macrophage-1 Antigen / Q000096:biosynthesis; D010972:Platelet Activating Factor / Q000494:pharmacology; D043684:Receptor, Adenosine A3; D018047:Receptors, Purinergic P1 / Q000187:drug effects / Q000502:physiology*
chemical_list: D018821:CD18 Antigens; D018946:Chemokine CCL5; D016177:Macrophage-1 Antigen; D010972:Platelet Activating Factor; D043684:Receptor, Adenosine A3; D018047:Receptors, Purinergic P1; D019041:L-Selectin; D007975:Leukotriene B4; D000241:Adenosine; C090034:2-chloro-N(6)-(3-iodobenzyl)adenosine-5'-N-methyluronamide