Isolation of a hamster cDNA homologous to the mouse and human cyclin kinase inhibitory protein p27Kip1.
We report here the isolation, cloning and sequencing of a hamster cDNA homologous to the mouse cyclin kinase inhibitory protein p27Kip1. The full length hamster cDNA sequence (p30Kip1)5 revealed 91% similarity with the previously reported mouse and human cDNAs and coded for a protein of 198 amino acids, results which were very similar to that observed for the mouse and human protein. Western blotting analysis performed using a polyclonal antibody against a mouse cyclin kinase inhibitory protein, p27Kip1, revealed the presence of a strongly reactive protein band at 30 kDa (as opposed to 27 kDa in mouse and human cells) in cell lysate prepared from rat and hamster cells. Although the size of the cyclin kinase inhibitory protein cDNA transcript is similar in mouse and hamster, it is likely that the differential mobility of the hamster p30Kip1 protein compared to the mouse and human p27Kip1 protein could be due to post-translational modifications.
Parekh
H
H
Department of Pathology, Temple University School of Medicine, Philadelphia, Pennsylvania 19140, USA.
Pillarisetti
K
K
Kunapuli
S
S
Simpkins
H
H
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002352:Carrier Proteins / Q000737:chemistry / Q000235:genetics* / Q000302:isolation & purification; D018797:Cell Cycle Proteins; D006224:Cricetinae; D050760:Cyclin-Dependent Kinase Inhibitor p27; D018844:Cyclin-Dependent Kinases / Q000037:antagonists & inhibitors*; D018076:DNA, Complementary / Q000302:isolation & purification*; D004791:Enzyme Inhibitors / Q000737:chemistry / Q000302:isolation & purification; D006801:Humans; D051379:Mice; D008969:Molecular Sequence Data; D017386:Sequence Homology, Amino Acid; D012689:Sequence Homology, Nucleic Acid; D025521:Tumor Suppressor Proteins
D002352:Carrier Proteins; C493961:Cdkn1b protein, mouse; D018797:Cell Cycle Proteins; D018076:DNA, Complementary; D004791:Enzyme Inhibitors; D025521:Tumor Suppressor Proteins; D050760:Cyclin-Dependent Kinase Inhibitor p27; D018844:Cyclin-Dependent Kinases