title: Immunization with the cross-reactive antigens Ov39 from Onchocerca volvulus and hr44 from human retinal tissue induces ocular pathology and activates retinal microglia.
abstract: Antigen Ov39, derived from Onchocerca volvulus, cross-reacts on both the T and B cell level with a nonhomologous human retinal antigen, hr44. Lewis rats were immunized to investigate the potential of these antigens to induce eye disease. Histologic and immunohistologic examination of ocular tissues revealed pathologic changes as early as day 12, which included induction or up-regulation of class II and CD68-like antigen on perivascular cells, ramified retinal microglia, dendritiform cells of the iris epithelium, and ciliary epithelium and significant breakdown of anterior and posterior blood-ocular barriers. Extravascular immunoglobulin and staining for CD68-like antigen was detected in the optic nerve after immunization with Ov39. Unrelated structural abnormalities of retina and lens seen in 8% of eyes examined significantly predisposed eyes to the development of Ov39- or hr44-induced pathology. These findings suggest a role for cross-reactive immune responses in the development of ocular onchocerciasis.
authors.affiliation: Department of Pathology, University of Cambridge, United Kingdom.
authors.forename: N M
authors.initials: NM
authors.lastname: McKechnie
authors.forename: W
authors.initials: W
authors.lastname: Gürr
authors.forename: G
authors.initials: G
authors.lastname: Braun
mesh_terms: D000818:Animals; D015703:Antigens, CD / Q000032:analysis; D015214:Antigens, Differentiation, Myelomonocytic / Q000032:analysis; D000947:Antigens, Helminth / Q000276:immunology*; D003429:Cross Reactions; D005123:Eye / Q000473:pathology; D005128:Eye Diseases / Q000209:etiology*; D005136:Eye Proteins / Q000276:immunology*; D005904:Glial Fibrillary Acidic Protein / Q000032:analysis; D000949:Histocompatibility Antigens Class II / Q000032:analysis; D006801:Humans; D007114:Immunization; D007150:Immunohistochemistry; D008297:Male; D008565:Membrane Proteins / Q000276:immunology*; D017628:Microglia / Q000473:pathology*; D017181:Onchocerca volvulus / Q000276:immunology*; D051381:Rats; D011917:Rats, Inbred Lew; D012160:Retina / Q000473:pathology*; D000097382:CD68 Molecule
chemical_list: D015703:Antigens, CD; D015214:Antigens, Differentiation, Myelomonocytic; D000947:Antigens, Helminth; D005136:Eye Proteins; D005904:Glial Fibrillary Acidic Protein; D000949:Histocompatibility Antigens Class II; D008565:Membrane Proteins; C067980:CD68 antigen, human; D000097382:CD68 Molecule; C095845:Ov39 antigen; C095844:hr44 antigen