HIV antibody assay that gave false negative results: multicentre collaborative study.
OBJECTIVE
To identify false negative results arising from the use of a commercial kit to detect antibody to HIV-1 and HIV-2 between July 1995 and March 1996.


METHODS
The 56 laboratories in the United Kingdom that were using the assay were asked to retrieve and retest specimens with an alternative assay for HIV-1 and HIV-2. Details of false negative results were obtained and these serum samples further investigated.


METHODS
24,181 patients tested with the assay who were reported as being negative for HIV antibody. An additional 497 patients were confirmed as HIV positive with the assay.


RESULTS
Serum samples of 20,973 of the patients were retested, and four patients were found to have had false negative results with the kit; three further patients were found to have had false negative results in the course of other laboratory testing. The seven patients with false negative results with the kit were of diverse risk group and HIV-1 subtype. Four had evidence of recent HIV infection.


CONCLUSIONS
The commercial kit had a sensitivity of 99.2% (497/501), or less if the additional three patients with false negative results were taken into account.
Evans
B G
BG
Public Health Laboratory Service, London. bevans@phls.co.uk
Parry
J V
JV
Mortimer
P P
PP
D005188:False Negative Reactions; D005260:Female; D015483:HIV Antibodies / Q000032:analysis*; D015658:HIV Infections / Q000175:diagnosis*; D015497:HIV-1 / Q000276:immunology*; D015498:HIV-2 / Q000276:immunology*; D006801:Humans; D008297:Male; D011933:Reagent Kits, Diagnostic / Q000592:standards*
D015483:HIV Antibodies; D011933:Reagent Kits, Diagnostic