title: Effects of 4-ipomeanol on bovine alveolar macrophage function.
abstract: The objective of this study was to determine whether 4-ipomeanol toxicosis in calves impairs alveolar macrophage functions important in pulmonary defense against infectious agents. Male Holstein calves were given either 4-ipomeanol (3 mg kg-1, i.v.) or vehicle (polyethylene glycol 400). Alveolar macrophages were recovered by pulmonary lavage 3 days later, and their capacities to phagocytose and kill E. coli, migrate toward zymosan-activated immune bovine serum, and produce interferon and interleukin-1 activity were evaluated in vitro. Alveolar macrophages recovered from 4-ipomeanol-treated calves had over a 70% decrease (p < 0.01) in chemotactic activity and over a 37% decrease (p < 0.005) in their capacity to phagocytose E. coli as compared to macrophages from control calves. Interleukin-1 activity in macrophages from 4-ipomeanol-treated calves tended to be higher than that from control calves, but the differences were not statistically significant (p = 0.06). 4-ipomeanol did not affect macrophage bactericidal activity or production of interferon. These results indicate that 4-ipomeanol suppresses select functions of alveolar macrophages in cattle that may be important in pulmonary defense against bacterial pathogens.
authors.affiliation: Department of Pathobiology, College of Veterinary Medicine, University of Florida, Gainesville 32610, USA.
authors.forename: X
authors.initials: X
authors.lastname: Li
authors.forename: C J
authors.initials: CJ
authors.lastname: Czuprynski
authors.forename: W L
authors.initials: WL
authors.lastname: Castleman
mesh_terms: D000818:Animals; D002417:Cattle; D002418:Cattle Diseases / Q000276:immunology / Q000503:physiopathology; D002633:Chemotaxis / Q000187:drug effects; D004926:Escherichia coli / Q000276:immunology; D005506:Food Contamination; D005517:Foodborne Diseases / Q000276:immunology / Q000503:physiopathology / Q000662:veterinary; D007372:Interferons / Q000096:biosynthesis; D007375:Interleukin-1 / Q000096:biosynthesis; D016676:Macrophages, Alveolar / Q000187:drug effects* / Q000276:immunology / Q000502:physiology*; D008297:Male; D010587:Phagocytosis / Q000187:drug effects; D013729:Terpenes / Q000494:pharmacology*
chemical_list: D007375:Interleukin-1; D013729:Terpenes; D007372:Interferons; C001470:4-ipomeanol