title: Preliminary NMR studies of Thermus thermophilus ribosomal protein S19 overproduced in Escherichia coli.
abstract: The gene for the ribosomal protein S19 from Thermus thermophilus was cloned, sequenced and overexpressed in Escherichia coli. A simple procedure for isolating the recombinant protein was developed. Preliminary NMR studies revealed a high content of alpha-helical secondary structure in the protein.
authors.affiliation: Institute of Protein Research, Russian Academy of Sciences, Pushchino, Moscow Region.
authors.forename: N L
authors.initials: NL
authors.lastname: Davydova
authors.forename: A V
authors.initials: AV
authors.lastname: Rak
authors.forename: O I
authors.initials: OI
authors.lastname: Gryaznova
authors.forename: A
authors.initials: A
authors.lastname: Liljas
authors.forename: B H
authors.initials: BH
authors.lastname: Jonsson
authors.forename: H
authors.initials: H
authors.lastname: Berglund
authors.forename: T
authors.initials: T
authors.lastname: Härd
authors.forename: M B
authors.initials: MB
authors.lastname: Garber
mesh_terms: D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000737:chemistry* / Q000235:genetics; D003001:Cloning, Molecular; D017931:DNA Primers; D004926:Escherichia coli / Q000235:genetics; D015870:Gene Expression; D009682:Magnetic Resonance Spectroscopy; D008969:Molecular Sequence Data; D017510:Protein Folding; D017433:Protein Structure, Secondary; D011994:Recombinant Proteins / Q000737:chemistry; D012269:Ribosomal Proteins / Q000737:chemistry* / Q000235:genetics; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D016963:Thermus thermophilus / Q000737:chemistry / Q000235:genetics*
chemical_list: D001426:Bacterial Proteins; D017931:DNA Primers; D011994:Recombinant Proteins; D012269:Ribosomal Proteins; C040343:ribosomal protein S19