Use of antibody-coated cellulose sponges for enhanced isolation of salmonella.
One thousand, four hundred and fifty-one naturally contaminated samples from pig, poultry and cattle farms, poultry hatcheries and animal feed mills were examined in a trial in which transfer of small portions of cellulose sponge coated with salmonella somatic polyvalent antiserum was compared with transfer of standard liquid inocula from pre-enrichment to selective enrichment culture. Salmonella was found in 281 (19.4%) of the samples using the standard method, compared with 385 (26.5%) using the sponge method. It was therefore concluded that antibody-coated cellulose sponges could be a simple means of increasing the recovery of salmonellas from pre-enrichment broths and thereby enhancing the test sensitivity.
Davies
R H
RH
Bacteriology Department, Central Veterinary Laboratory, Addlestone, Surrey, UK.
Wray
C
C
D000821:Animal Feed / Q000382:microbiology; D000822:Animal Husbandry; D000818:Animals; D000907:Antibodies, Bacterial; D001431:Bacteriological Techniques; D002417:Cattle; D002482:Cellulose; D005069:Evaluation Studies as Topic; D005516:Food Microbiology; D011161:Porifera; D011200:Poultry; D012475:Salmonella / Q000276:immunology / Q000302:isolation & purification*; D013552:Swine
D000907:Antibodies, Bacterial; D002482:Cellulose