title: Polymorphism at codons 114, 116, 145, and 163 muddle the typing of HLA-B*1304.
abstract: Genetic exchanges often muddle the typing of HLA class I molecules, this is also the case for HLA-B*1304. Serologic and molecular DNA class I typing report a B15/B55 type for cell 847, whereas DNA sequencing finds B*5501/B*1304. HLA-B*1304 differs by no more than four amino acids from other HLA-B13 molecules, a comparative analysis of the B13 and B15 families was therefore performed to determine why serologic and molecular DNA approaches report a B15 type for B*1304. Comparisons demonstrate that limited differences individuate the B15 and B13 molecules such that the genetic recombination of codons 145 and 163 in the class I heavy chain's alpha 2 alpha helix prompt B*1304 to exhibit a B15X21 pattern of serologic cross-reactivity. Molecular DNA class I typing approaches are also swayed by genetic recombinations to type B*1304 as a B15 molecule: B15-like nucleotide sequences encoding residues 114, 116, and 145, lead B*1304 to exhibit a B15 PCR amplification pattern. Thus, genetic exchanges encoding key amino acids in the class I heavy chain lead molecular and serologic typing approaches to categorize HLA-B*1304 as a member of the B15 family.
authors.affiliation: Department of Microbiology and Immunology, University of Oklahoma Health Sciences Center, Oklahoma City 73190, USA.
authors.forename: M
authors.initials: M
authors.lastname: Ellexson
authors.forename: P
authors.initials: P
authors.lastname: Lai-Kwan
authors.forename: M
authors.initials: M
authors.lastname: Lau
authors.forename: K
authors.initials: K
authors.lastname: Muto
authors.forename: P
authors.initials: P
authors.lastname: Terasaki
authors.forename: J
authors.initials: J
authors.lastname: Cole
authors.forename: C
authors.initials: C
authors.lastname: Thompson
authors.forename: W
authors.initials: W
authors.lastname: Hildebrand
mesh_terms: D000483:Alleles; D000595:Amino Acid Sequence; D001483:Base Sequence; D003062:Codon; D003429:Cross Reactions; D005838:Genotype; D015235:HLA-B Antigens / Q000235:genetics* / Q000276:immunology; D059908:HLA-B13 Antigen; D059910:HLA-B15 Antigen; D006650:Histocompatibility Testing; D006801:Humans; D008969:Molecular Sequence Data; D011110:Polymorphism, Genetic
chemical_list: D003062:Codon; D015235:HLA-B Antigens; D059908:HLA-B13 Antigen; D059910:HLA-B15 Antigen; C063569:HLA-B21 antigen