Evaluation of the effect of macrophage system activation on the intensity degree of early destructive changes in acute enzymatic lung injury.
The effect of macrophage system activation on the intensity degree of early destructive changes induced with intratracheal infusion of a proteolytic enzyme-papain was evaluated in the paper. The study was carried out on male Wistar rats. BCG vaccine was used as a macrophage system activating substance. Damage degree of the pulmonary tissue was evaluated after 2 hours following papain administration by determining W/D index (wet lungs/dry lungs). Spectrophometric analysis of bronchoalveolar lavages (BAL) was made in order to evaluate the advancement of haemorrhagic alterations in the lungs. Damage to the pulmonary tissue was greater in the animals given BCG vaccine and papain, compared with controls and papain treated animals. Differences in the intensity degree of destructive changes assessed using both the W/D index and haemoglobin content in BAL were statistically significant. The results obtained indicate a particular role of activation of the macrophage system in the development of early destructive changes due to intratracheal administration of proteolytic enzymes and point to the significance of these changes in the morphogenesis of experimental lung emphysema.
Sulkowski
S
S
Department of Pathological Anatomy, Medical Academy of Białystok.
Sulkowska
M
M
Giedrojć
J
J
Terlikowski
S
S
Dziecioł
J
J
Famulski
W
W
D000818:Animals; D001500:BCG Vaccine / Q000494:pharmacology; D001992:Bronchoalveolar Lavage Fluid; D004646:Emphysema / Q000139:chemically induced / Q000473:pathology*; D006470:Hemorrhage / Q000139:chemically induced / Q000473:pathology*; D008168:Lung / Q000187:drug effects* / Q000473:pathology; D008262:Macrophage Activation / Q000187:drug effects; D016676:Macrophages, Alveolar / Q000502:physiology*; D008297:Male; D010206:Papain / Q000633:toxicity*; D011654:Pulmonary Edema / Q000139:chemically induced / Q000473:pathology*; D051381:Rats; D017208:Rats, Wistar
D001500:BCG Vaccine; D010206:Papain