Binding characteristics of SLE anti-DNA autoantibodies to modified DNA analogues.
Native calf thymus DNA was modified with furocoumarins and reactive oxygen species (ROS). The modifications were probed by UV & Fluorescence spectroscopy, thermal denaturation and hydroxyapatite chromatography. In CD spectroscopy changes in the ellipticity of the DNA molecule were observed as a result of modification. The binding specificity of naturally occurring anti-DNA antibodies with modified DNA molecules was assessed by ELISA and quantitative precipitin titration. The affinity of anti-DNA antibodies for modified conformers was found to be quite high.
Arjumand
S
S
Department of Biochemistry, Faculty of Medicine, A.M.U., Aligarh, India.
Moinuddin
Ali
A
A
D000974:Antibodies, Antinuclear / Q000378:metabolism*; D000918:Antibody Specificity; D002942:Circular Dichroism; D018736:DNA Adducts / Q000187:drug effects / Q000378:metabolism* / Q000528:radiation effects; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D008180:Lupus Erythematosus, Systemic / Q000188:drug therapy / Q000276:immunology*
D000974:Antibodies, Antinuclear; D018736:DNA Adducts