An immuno-dot blot assay for detection of thermostable protease from Pseudomonas sp. AFT-36 of dairy origin.
A dot-ELISA technique for the detection of Pseudomonas protease was developed using IgG of anti-Pseudomonas AFT-36 protease as capture antibody. The detection limit of protease in buffer or milk was 1.01 ng ml-1. The procedure was performed at room temperature, took about 2.5 h and was economical. Protease AFT-36 is immunologically related to five out of seven Pseudomonas spp. The results suggest that the assay could be used to detect proteases in dairy products.
Matta
H
H
Department of Microbiology, College of Basic Sciences, Himachal Pradesh Agricultural University, Palampur, India.
Punj
V
V
Kanwar
S S
SS
D000818:Animals; D000918:Antibody Specificity; D003080:Cold Temperature; D003429:Cross Reactions; D003611:Dairy Products / Q000382:microbiology*; D010450:Endopeptidases / Q000032:analysis* / Q000276:immunology; D004795:Enzyme Stability; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods* / Q000706:statistics & numerical data; D005069:Evaluation Studies as Topic; D011549:Pseudomonas / Q000201:enzymology* / Q000302:isolation & purification*; D012680:Sensitivity and Specificity; D013696:Temperature
D010450:Endopeptidases