Induction of the expression of gag protein in HTLV-I infected lymphocytes by anti-ICAM 1 antibody in vitro.
Intercellular adhesion molecule 1 (ICAM 1) is an inducible protein ligand which is up-regulated during inflammation and is either not constitutively expressed or is only expressed at low levels. The expression of ICAM 1 increases in HTLV-I infected T cell lines as well as in CD4+ T-cells of peripheral blood lymphocytes (PBLs) from HAM/TSP patients. After PBLs of HAM/TSP patients were cultured in the presence of stimulating anti-ICAM 1 antibody, the expression of the HTLV-I gag protein in PBLs was observed by both immuno-histostaining and western blot analysis using HTLV-I Ag-specific mouse monoclonal antibody. These data thus suggested that the signal transduction via adhesion molecule, ICAM 1 could induce the transcription of the HTLV-I gene and this might therefore play an important role in the pathogenesis of HAM/TSP.
Yamamoto
A
A
Department of Neurology, Neurological Institute, Faculty of Medicine, Kyusyu University, Fukuoka, Japan.
Hara
H
H
Kobayashi
T
T
D000328:Adult; D000906:Antibodies / Q000494:pharmacology*; D015153:Blotting, Western; D015496:CD4-Positive T-Lymphocytes / Q000378:metabolism*; D002460:Cell Line; D002478:Cells, Cultured; D005434:Flow Cytometry; D015683:Gene Products, gag / Q000096:biosynthesis*; D006801:Humans; D007150:Immunohistochemistry; D018799:Intercellular Adhesion Molecule-1 / Q000276:immunology*; D015493:Paraparesis, Tropical Spastic / Q000276:immunology / Q000378:metabolism*; D015398:Signal Transduction / Q000502:physiology; D015854:Up-Regulation
D000906:Antibodies; D015683:Gene Products, gag; D018799:Intercellular Adhesion Molecule-1