title: Regulation of the yeast HIS7 gene by the global transcription factor Abf1p.
abstract: The HIS7 gene of Saccharomyces cerevisiae encodes a bifunctional glutamine amidotransferase: cyclase that catalyzes the formation of biosynthetic precursors for histidine and adenine. HIS7 is activated by Gcn4p upon amino acid starvation and by the Bas1/2p complex in response to adenine limitation. Mutation analysis of the HIS7 promoter in a deltagcn4 background revealed a polyd(A/T) stretch and a d(CT) repeat as essential elements for Gcn4p-independent basal HIS7 transcription. The protein binding this element was enriched and identified as the multifunctional DNA-binding protein Abf1p. Abf1p binds specifically to the d(CT) repeat sequence, which represents a novel Abf1p-binding motif, and protects 17 nucleotides from digestion by DNase I. In addition, Abf1p binding causes bending of the HIS7 promoter structure.
authors.affiliation: Institut für Mikrobiologie und Genetik Georg-August-Universität, Göttingen, Germany.
authors.forename: C
authors.initials: C
authors.lastname: Springer
authors.forename: S
authors.initials: S
authors.lastname: Krappmann
authors.forename: M
authors.initials: M
authors.lastname: Künzler
authors.forename: C
authors.initials: C
authors.lastname: Zmasek
authors.forename: G H
authors.initials: GH
authors.lastname: Braus
mesh_terms: D000619:Aminohydrolases; D003001:Cloning, Molecular; D004271:DNA, Fungal / Q000235:genetics; D004268:DNA-Binding Proteins / Q000235:genetics* / Q000378:metabolism*; D003850:Deoxyribonuclease I / Q000378:metabolism; D018900:Dinucleotide Repeats; D005656:Fungal Proteins / Q000235:genetics; D015966:Gene Expression Regulation, Fungal; D007763:Lac Operon; D009097:Multienzyme Complexes / Q000235:genetics* / Q000378:metabolism*; D016297:Mutagenesis, Site-Directed; D011061:Poly A / Q000235:genetics; D011071:Poly T / Q000235:genetics; D016133:Polymerase Chain Reaction; D011401:Promoter Regions, Genetic; D014176:Protein Biosynthesis; D011494:Protein Kinases / Q000235:genetics; D012091:Repetitive Sequences, Nucleic Acid; D012441:Saccharomyces cerevisiae / Q000235:genetics*; D029701:Saccharomyces cerevisiae Proteins; D017384:Sequence Deletion; D015534:Trans-Activators / Q000235:genetics; D014157:Transcription Factors / Q000235:genetics* / Q000378:metabolism*; D014158:Transcription, Genetic; D014166:Transferases / Q000235:genetics* / Q000378:metabolism*; D001616:beta-Galactosidase / Q000378:metabolism
chemical_list: C061886:ABF1 protein, S cerevisiae; C061767:BAS1 protein, S cerevisiae; D004271:DNA, Fungal; D004268:DNA-Binding Proteins; D005656:Fungal Proteins; D009097:Multienzyme Complexes; D029701:Saccharomyces cerevisiae Proteins; D015534:Trans-Activators; D014157:Transcription Factors; D011061:Poly A; D011071:Poly T; C008950:poly A-T; D014166:Transferases; D011494:Protein Kinases; D003850:Deoxyribonuclease I; D001616:beta-Galactosidase; C087227:imidazole glycerol phosphate synthase; D000619:Aminohydrolases