title: Phenotypic and genotypic biotyping of environmental strains of Vibrio cholerae non-O1 isolated in Italy.
abstract: The purpose of this study was to characterize strains of Vibrio cholerae non-O1 isolated in Italy from different sources by biochemical and serological assays, antibiotic susceptibility testing, and molecular biotyping. Serotyping and genomic analysis by pulsed-field gel electrophoresis proved to be useful in discriminating the isolates. The data obtained show a wide heterogeneity at the genomic level, and in keeping with this, the serogrouping classification provided evidence of a high variability of the investigated strains. In addition, none of the strains tested produced cholera-like toxins.
authors.affiliation: Istituto Superiore di Sanità, Rome, Italy.
authors.forename: E
authors.initials: E
authors.lastname: Filetici
authors.forename: L
authors.initials: L
authors.lastname: Bonadonna
authors.forename: M
authors.initials: M
authors.lastname: Ciccozzi
authors.forename: M P
authors.initials: MP
authors.lastname: Anastasio
authors.forename: M
authors.initials: M
authors.lastname: Fantasia
authors.forename: T
authors.initials: T
authors.lastname: Shimada
mesh_terms: D000818:Animals; D015373:Bacterial Typing Techniques; D002772:Cholera Toxin / Q000235:genetics; D004269:DNA, Bacterial / Q000235:genetics / Q000302:isolation & purification; D004352:Drug Resistance, Microbial; D016521:Electrophoresis, Gel, Pulsed-Field; D004783:Environmental Microbiology; D005798:Genes, Bacterial; D005838:Genotype; D006801:Humans; D007558:Italy; D010641:Phenotype; D012703:Serotyping; D014734:Vibrio cholerae / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification
chemical_list: D004269:DNA, Bacterial; D002772:Cholera Toxin