title: Expression of lymphocyte homing receptors and vascular addressins in low-grade gastric B-cell lymphomas of mucosa-associated lymphoid tissue.
abstract: In this study, we examined lymphocyte homing receptor and vascular addressin expression in a case of primary gastric B-cell lymphoma of mucosa-associated lymphoid tissue (MALT) with a secondary intestinal spread. We compared the findings with that observed in B cells of normal MALT and MALT acquired as a consequence of Helicobacter pylori-associated gastritis and other low-grade gastric B-cell MALT lymphomas. The neoplastic B cells in the gastric tumor were alpha 4 beta 7-, CD62L+, whereas the intestinal secondary was alpha 4 beta 7+, CD62L-. Incubation of isolated tumor cells from the stomach by H. pylori generated T-cell-dependent proliferation of neoplastic B cells and induced expression of alpha 4 beta 7 integrin similar to the intestinal tumor. These observations indicate that reversal of homing receptor profile in the gastric tumor by antigen specific stimulation may be responsible for secondary intestinal dissemination. In normal stomach and normal MALT, alpha 4 beta 7 and CD62L expression reflected the differentiation of the B cell. Plasma cells were alpha 4 beta 7+, CD62L-, whereas a subset of memory B cells were alpha 4 beta 7-, CD62L+. Homing receptor expression in MALT lymphoma B cells was heterogeneous, however, in line with their memory B-cell phenotype in the majority of cases, the neoplastic B cells were alpha 4 beta 7-, CD62L+. Neoplastic plasma cells were always alpha 4 beta 7+, CD62L-. The venules in normal gastric mucosa expressed mucosal addressin cell adhesion molecule-1 but not peripheral lymph node addressin. In normal MALT, H. pylori-associated follicular gastritis and MALT lymphomas high endothelial venules coexpressed mucosal addressin cell adhesion molecule-1 and peripheral lymph node addressin. These findings suggest expression of lymphocyte homing receptors by B cells and vascular addressins by mucosal venules are similar in normal MALT and MALT lymphomas, and factors controlling normal mucosal B-cell traffic are also operational in MALT lymphomas.
authors.affiliation: Department of Histopathology, University College London Medical School, United Kingdom. a.dogan@ucl.ac.uk
authors.forename: A
authors.initials: A
authors.lastname: Dogan
authors.forename: M
authors.initials: M
authors.lastname: Du
authors.forename: A
authors.initials: A
authors.lastname: Koulis
authors.forename: M J
authors.initials: MJ
authors.lastname: Briskin
authors.forename: P G
authors.initials: PG
authors.lastname: Isaacson
mesh_terms: D000954:Antigens, Surface / Q000378:metabolism*; D001402:B-Lymphocytes / Q000378:metabolism / Q000473:pathology; D002448:Cell Adhesion / Q000502:physiology; D015815:Cell Adhesion Molecules; D005260:Female; D005756:Gastritis / Q000378:metabolism / Q000382:microbiology; D016481:Helicobacter Infections / Q000378:metabolism; D016480:Helicobacter pylori / Q000502:physiology; D006801:Humans; D007136:Immunoglobulins / Q000235:genetics / Q000378:metabolism; D016023:Integrins / Q000378:metabolism; D007414:Intestinal Neoplasms / Q000556:secondary; D019041:L-Selectin / Q000378:metabolism; D018442:Lymphoma, B-Cell, Marginal Zone / Q000378:metabolism* / Q000382:microbiology / Q000473:pathology; D008565:Membrane Proteins; D008875:Middle Aged; D009088:Mucoproteins / Q000235:genetics / Q000378:metabolism; D016201:Receptors, Lymphocyte Homing / Q000378:metabolism*; D013274:Stomach Neoplasms / Q000378:metabolism* / Q000382:microbiology / Q000473:pathology; D014162:Transfection
chemical_list: D000954:Antigens, Surface; D015815:Cell Adhesion Molecules; D007136:Immunoglobulins; D016023:Integrins; C069886:L-selectin counter-receptors; C504265:MADCAM1 protein, human; D008565:Membrane Proteins; D009088:Mucoproteins; D016201:Receptors, Lymphocyte Homing; C071777:integrin alpha4beta7; D019041:L-Selectin