title: Microvilli elongate in response to hydrogen peroxide and to perturbations of intracellular calcium.
abstract: Using scanning electron microscopy and fluorescence microscopy, we have found that apical microvilli of diverse cell types, including nonepithelial cells, elongate in culture in response to the oxidative stress of hydrogen peroxide. The microvilli induced in culture on retinal pigment epithelial cells display a 30-nm axial periodicity similar to that described for stable microvilli of intestinal brush border. Microvilli can also be induced to elongate by chelating intracellular Ca2+ and by the Ca(2+)-uptake inhibitor thapsigargin. Thus a response of microvillar protrusion occurs widely and may be related to depletion of intracellular calcium stores.
authors.affiliation: Institute of Biomedical and Life Sciences, University of Glasgow, United Kingdom.
authors.forename: G G
authors.initials: GG
authors.lastname: Reid
authors.forename: J G
authors.initials: JG
authors.lastname: Edwards
authors.forename: G E
authors.initials: GE
authors.lastname: Marshall
authors.forename: R G
authors.initials: RG
authors.lastname: Sutcliffe
authors.forename: W R
authors.initials: WR
authors.lastname: Lee
mesh_terms: D000634:Aminoquinolines; D000818:Animals; D000001:Calcimycin / Q000494:pharmacology; D002118:Calcium / Q000378:metabolism* / Q000494:pharmacology; D002460:Cell Line; D002462:Cell Membrane / Q000502:physiology; D002614:Chelating Agents; D004533:Egtazic Acid / Q000031:analogs & derivatives; D005456:Fluorescent Dyes; D006801:Humans; D006861:Hydrogen Peroxide / Q000494:pharmacology*; D007202:Indicators and Reagents; D007476:Ionophores / Q000494:pharmacology; D008854:Microscopy, Electron; D008855:Microscopy, Electron, Scanning; D008871:Microvilli / Q000187:drug effects / Q000502:physiology* / Q000648:ultrastructure; D015388:Organelles / Q000502:physiology; D010857:Pigment Epithelium of Eye / Q000166:cytology* / Q000187:drug effects / Q000648:ultrastructure; D015398:Signal Transduction / Q000502:physiology; D014969:Xanthine Oxidase / Q000494:pharmacology
chemical_list: D000634:Aminoquinolines; D002614:Chelating Agents; D005456:Fluorescent Dyes; D007202:Indicators and Reagents; D007476:Ionophores; D000001:Calcimycin; D004533:Egtazic Acid; D006861:Hydrogen Peroxide; D014969:Xanthine Oxidase; C025603:1,2-bis(2-aminophenoxy)ethane-N,N,N',N'-tetraacetic acid; C035739:Quin2; D002118:Calcium