title: The human transaldolase gene (TALDO1) is located on chromosome 11 at p15.4-p15.5.
abstract: Transaldolase (TAL) is a key enzyme of the pentose phosphate pathway, which is responsible for generation of reducing equivalents to protect cellular integrity from reactive oxygen intermediates. While exons 2 and 3 are highly repetitive, the complete TAL-H gene is mapped to a single genomic locus (TALDO1(2)) by several independent approaches. Southern blot hybridization of a 827-bp 3' EcoRI fragment of the TAL-H cDNA to human-mouse somatic cell hybrid DNA localized TALDO1 to the p13-->pter region of chromosome 11. Fluorescence in situ hybridization with a 15-kb genomic fragment harboring exons 1 and 2 mapped TALDO1 to 11p15.4-p15.5. A truncated and mutated segment of TAL-H exon 5 terminating with a poly(A) tail was identified in a pseudogene locus (TALDOP1) on chromosome 1. Reverse transcriptase-PCR studies of human-mouse somatic cell hybrids revealed the presence of the functional TAL-H gene on chromosome 11 and its absence on human chromosome 1. Mapping of radiation hybrids placed TALDO1 between markers WI-1421 and D11S922 on 11p15.
authors.affiliation: Department of Medicine, State University of New York Health Science Center, College of Medicine, Syracuse 13210, USA.
authors.forename: K
authors.initials: K
authors.lastname: Banki
authors.forename: R L
authors.initials: RL
authors.lastname: Eddy
authors.forename: T B
authors.initials: TB
authors.lastname: Shows
authors.forename: D L
authors.initials: DL
authors.lastname: Halladay
authors.forename: F
authors.initials: F
authors.lastname: Bullrich
authors.forename: C M
authors.initials: CM
authors.lastname: Croce
authors.forename: V
authors.initials: V
authors.lastname: Jurecic
authors.forename: A
authors.initials: A
authors.lastname: Baldini
authors.forename: A
authors.initials: A
authors.lastname: Perl
mesh_terms: D001483:Base Sequence; D002874:Chromosome Mapping; D002880:Chromosomes, Human, Pair 11; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D008969:Molecular Sequence Data; D012689:Sequence Homology, Nucleic Acid; D014153:Transaldolase / Q000235:genetics*
chemical_list: D014153:Transaldolase