Increases in mRNA levels of glucose transporters types 1 and 3 in Ehrlich ascites tumor cells during tumor development.
A common feature of many tumors is an increase in glucose catabolism during tumor growth. We studied the mechanism of this phenomenon by using Ehrlich ascites tumor bearing mice as the animal model. We found that Ehrlich ascites tumor cells possess only glucose transporter 1 (GLUT1) and GLUT3 but not GLUT2, GLUT4, or GLUT5. The mRNA levels of GLUT1 and GLUT3 increased progressively in the tumour during development; however, there were no changes observable in mRNA levels of glucose transporters of all types in brain, liver, and heart of the host mice. These findings suggest that Ehrlich ascites tumor augments its glucose transport mechanism relative to other tissues in response to its unique growth needs.
Au
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KK
Department of Biochemistry, Chinese University of Hong Kong, Shatin, N.T., China.
Liong
E
E
Li
J Y
JY
Li
P S
PS
Liew
C C
CC
Kwok
T T
TT
Choy
Y M
YM
Lee
C Y
CY
Fung
K P
KP
D000818:Animals; D001786:Blood Glucose / Q000032:analysis; D002286:Carcinoma, Ehrlich Tumor / Q000235:genetics* / Q000378:metabolism; D018076:DNA, Complementary; D005508:Food Deprivation; D015972:Gene Expression Regulation, Neoplastic / Q000502:physiology; D051272:Glucose Transporter Type 1; D051274:Glucose Transporter Type 3; D006801:Humans; D051379:Mice; D009004:Monosaccharide Transport Proteins / Q000235:genetics*; D009419:Nerve Tissue Proteins; D009928:Organ Specificity; D012333:RNA, Messenger / Q000096:biosynthesis*
D001786:Blood Glucose; D018076:DNA, Complementary; D051272:Glucose Transporter Type 1; D051274:Glucose Transporter Type 3; D009004:Monosaccharide Transport Proteins; D009419:Nerve Tissue Proteins; D012333:RNA, Messenger; C495677:SLC2A1 protein, human; C495687:SLC2A3 protein, human; C495678:Slc2a1 protein, mouse; C495689:Slc2a3 protein, mouse