CD10 is expressed on human thymic epithelial cell lines and modulates thymopentin-induced cell proliferation.
Thymic hormones such as thymopoietin (TP) have been shown to regulate thymocyte differentiation and lymphocyte activation. However, it is not known whether thymopoietin affects thymic epithelial cell (TEC) functions. In this study we have examined the effect of a five amino acid active peptide (TP5), corresponding to amino acids 32-36 of TP, on the proliferation of nontransformed clones of human TEC. Our results indicate that TP5 induced reinitiation of DNA synthesis and potentiated fetal calf serum (FCS)-induced cell growth in postnatal and fetal-derived human TEC. We also found that TEC lines express high levels of endopeptidase 24.11, a cell-surface metallopeptidase also known as the CD10 antigen. We show that TP5 is cleaved by CD10 at the surface of TEC lines, indicating that this endopeptidase may regulate TP5-induced TEC proliferation. Phosphoramidon, a specific endopeptidase 24.11 inhibitor, consistently acts in synergy with TP5 to enhance FCS-induced TEC growth. Hence, we conclude that 1) TP5 alone or in combination with FCS supports the growth of TEC lines, and 2) TEC lines express high levels of CD10, which regulates TP5-induced TEC proliferation by acting as a thymic peptide degrading enzyme.
Guérin
S
S
CJF INSERM 96.05, Faculté de Médecine, Nice, France.
Mari
B
B
Fernandez
E
E
Belhacene
N
N
Toribio
M L
ML
Auberger
P
P
D015703:Antigens, CD / Q000096:biosynthesis* / Q000502:physiology; D002455:Cell Division / Q000187:drug effects; D002460:Cell Line; D004247:DNA / Q000096:biosynthesis; D004847:Epithelial Cells / Q000166:cytology / Q000187:drug effects / Q000502:physiology*; D006801:Humans; D007700:Kinetics; D015260:Neprilysin / Q000096:biosynthesis* / Q000502:physiology; D011480:Protease Inhibitors / Q000494:pharmacology; D017154:Stromal Cells / Q000166:cytology / Q000276:immunology; D016305:Thymopentin / Q000494:pharmacology*; D013950:Thymus Gland / Q000166:cytology* / Q000276:immunology / Q000502:physiology
D015703:Antigens, CD; D011480:Protease Inhibitors; D004247:DNA; D015260:Neprilysin; D016305:Thymopentin