title: Successful reconstitution of human hematopoiesis in the SCID-hu mouse by genetically modified, highly enriched progenitors isolated from fetal liver.
abstract: Highly purified CD34++CD38-Lin- hematopoietic progenitors isolated from human fetal liver were infected with the murine retroviral vector, MFG nls-LacZ, which encodes a modified version of the Escherichia coli beta-galactosidase gene. Progenitors that were cocultured with the packaging cell line could reconstitute human bone marrow or thymus implanted in SCID-hu mice. Expression of the beta-galactosidase gene was observed in primitive and committed clonogenic progenitors, mature myeloid, B-lineage cells, and T-lineage cells for up to 4 months after injection into SCID-hu mice. Furthermore, hematopoietic reconstitution by genetically modified progenitor cells could be achieved by the injection of the cells generated from as few as 500 CD34++CD38-Lin- cells, suggesting efficient retroviral gene transfer into fetal liver progenitors.
authors.affiliation: Laboratoire de Biologie Cellulaire, Centre de Thérapie Génique, Institut Paoli-Calmettes, Marseille, France.
authors.forename: L
authors.initials: L
authors.lastname: Humeau
authors.forename: C
authors.initials: C
authors.lastname: Chabannon
authors.forename: M T
authors.initials: MT
authors.lastname: Firpo
authors.forename: P
authors.initials: P
authors.lastname: Mannoni
authors.forename: C
authors.initials: C
authors.lastname: Bagnis
authors.forename: M G
authors.initials: MG
authors.lastname: Roncarolo
authors.forename: R
authors.initials: R
authors.lastname: Namikawa
mesh_terms: D000818:Animals; D001854:Bone Marrow Cells / Q000166:cytology; D018920:Coculture Techniques; D005260:Female; D016332:Fetal Tissue Transplantation; D018014:Gene Transfer Techniques; D005822:Genetic Vectors; D006410:Hematopoiesis; D018380:Hematopoietic Stem Cell Transplantation; D006412:Hematopoietic Stem Cells / Q000166:cytology* / Q000502:physiology; D006801:Humans; D008099:Liver / Q000166:cytology* / Q000196:embryology; D051379:Mice; D016513:Mice, SCID; D011247:Pregnancy; D012190:Retroviridae