Oral immunization with recombinant vaccina expressing cell-surface-anchored beta hCG induces anti-hCG antibodies and T-cell proliferative response in rats.
A vaccinia recombinant, VSS2, expressing cell-surface-anchored beta-subunit of human chorionic gonadotropin (beta hCG) has earlier been found to induce high titered anti-hCG antibodies in rats immunized by tail scarification. The immunogenicity of this recombinant was compared in rats which received the virus through different routes of inoculation: intradermal, intragastric, intrajejunal or by tail scarification. The recombinant virus induced high titers of anti-hCG antibodies in all instances although the titers were about one log lower when the recombinant virus was delivered orally. The recombinant was found to induce T-cell proliferative response in rats of all immunization groups.
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National Institute of Immunology, Aruna Asaf Ali Marg, New Delhi, India.
Vrati
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D000818:Animals; D000917:Antibody Formation; D018997:Chorionic Gonadotropin, beta Subunit, Human / Q000276:immunology*; D003267:Contraception; D005260:Female; D007114:Immunization; D008213:Lymphocyte Activation; D051381:Rats; D017208:Rats, Wistar; D013601:T-Lymphocytes / Q000276:immunology*; D014614:Vaccines, Synthetic / Q000276:immunology*; D014615:Vaccinia / Q000235:genetics*
D018997:Chorionic Gonadotropin, beta Subunit, Human; D014614:Vaccines, Synthetic