Immunohistochemical analysis of heme oxygenase-I in rat liver after ischemia.
Total hepatic ischemia was induced by clamping the hepatic artery, portal vein, and bile duct. After 15 min hepatic ischemia, we examined a time course of the changes in the steady-state levels of heme oxygenase-1 (HO-1) mRNA and protein in the livers. The levels of HO-1 mRNA was transiently induced and peaked at 4 h after the start of reperfusion. In addition, a pattern of the time course of HO-1 protein levels was similar to that of HO-1 mRNA levels. Immunohistochemical analysis clearly showed that the localization of HO-1 protein induced by hepatic ischemia was restricted to the hepatocytes in the pericentral vein.
Ito
K
K
Department of Surgery, Tsuchiura Kyodo Hospital, Japan.
Ikeda
S
S
Shibata
T
T
Yano
T
T
Horikawa
S
S
D000818:Animals; D015152:Blotting, Northern; D015153:Blotting, Western; D004789:Enzyme Activation; D006419:Heme Oxygenase (Decyclizing) / Q000302:isolation & purification / Q000378:metabolism*; D051547:Heme Oxygenase-1; D007150:Immunohistochemistry / Q000379:methods; D007511:Ischemia / Q000201:enzymology*; D008099:Liver / Q000098:blood supply* / Q000201:enzymology; D008297:Male; D051381:Rats; D017208:Rats, Wistar; D015427:Reperfusion Injury / Q000201:enzymology*
D006419:Heme Oxygenase (Decyclizing); D051547:Heme Oxygenase-1