title: Hinnavin I, an antibacterial peptide from cabbage butterfly, Artogeia rapae.
abstract: We have previously isolated four antibacterial peptides from the immune haemolymph of the fifth instar larvae of cabbage butterfly, Artogeia rapae [Yoe, S. M., Bang, I. S., Kang, C. S., and Kim, H. J. (1996) Mol. Cells 6, 609-614]. They were induced by live, nonpathogenic gram negative bacteria. One of these novel antibacterial peptides was named hinnavin I. Hinnavin I is heat stable; its activity was retained after 60 min incubation at 100 degrees C, being effective against gram negative and/or gram positive bacteria. Hinnavin I and lysozyme II showed a powerful synergistic effect on the inhibition of bacterial growth. Amino acid composition was analyzed and the molecular weight was determined to be 4,139.94+/-10.91 Da by the ESI mass spectrometer. To elucidate the primary structure of hinnavin I, the amino acid sequence of this peptide was determined by N-terminal sequencing techniques. The amino-terminal half of the molecule was rich in charged amino acids and was hydrophilic, whereas the carboxyl-terminal half was hydrophobic.
authors.affiliation: Department of Biological Sciences, Dankook University, Cheonan, Korea.
authors.forename: I S
authors.initials: IS
authors.lastname: Bang
authors.forename: S Y
authors.initials: SY
authors.lastname: Son
authors.forename: S M
authors.initials: SM
authors.lastname: Yoe
mesh_terms: D000595:Amino Acid Sequence; D000596:Amino Acids / Q000032:analysis; D000818:Animals; D000900:Anti-Bacterial Agents / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology; D002080:Butterflies / Q000737:chemistry*; D004357:Drug Synergism; D004926:Escherichia coli / Q000187:drug effects / Q000254:growth & development; D006131:Growth Inhibitors / Q000494:pharmacology; D006458:Hemolymph / Q000737:chemistry; D019476:Insect Proteins / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology; D008969:Molecular Sequence Data; D008970:Molecular Weight; D009113:Muramidase / Q000494:pharmacology; D010455:Peptides / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology; D017421:Sequence Analysis
chemical_list: D000596:Amino Acids; D000900:Anti-Bacterial Agents; D006131:Growth Inhibitors; D019476:Insect Proteins; D010455:Peptides; C108747:hinnavin I protein, Artogeia rapae; D009113:Muramidase