Cytosine methylation inhibits expression of hsp-CAT gene in Drosophila cells.
Chloramphenicol acetyl transferase gene under control of hsp promoter (hsp-CAT gene) was introduced and expressed upon heat shock in Drosophila cells at 48 and 72 hr following transfection. Expression of CAT gene was remarkably reduced when DNA methylated at CpG sites was used although presence of methylated plasmid DNA could be demonstrated in cells at 48 and 72 hr. Thus, in Drosophila cells exogenously introduced methylated DNA is expressed differently from unmethylated DNA.
Deobagkar
D D
DD
Department of Zoology, University of Pune, India.
Vilekar
N
N
D000818:Animals; D015500:Chloramphenicol O-Acetyltransferase / Q000235:genetics*; D003596:Cytosine / Q000378:metabolism*; D019175:DNA Methylation; D004331:Drosophila melanogaster / Q000235:genetics*; D015971:Gene Expression Regulation, Enzymologic / Q000502:physiology*; D017344:Genes, Insect; D011401:Promoter Regions, Genetic
D003596:Cytosine; D015500:Chloramphenicol O-Acetyltransferase