Effects of membrane fatty acids on thermal and oxidative injury in the human premonocytic line U937.
Heat shock (HS) proteins (HSP) function as molecular chaperones and protect cells from thermal and oxidative injury. The signals leading to HSP synthesis, i.e. the "cellular thermometer(s)," are still a matter of debate. In the human premonocytic line U937, we investigated the effects of specific modification of membrane fatty acid (FA) composition by incubation with various saturated and unsaturated fatty acids (UFA) on the HS response and on hydrogen peroxide (H2O2)-induced cell death. FA readily incorporated into U937 cell membranes. UFA did not modulate the HS response but potentiated H2O2-mediated damage, while pre-exposure to HS protected the UFA-treated cells from this increased H2O2 toxicity.
Polla
B S
BS
Laboratoire de Physiologie Respiratoire, UFR Cochin Port-Royal, Universite Paris V, France.
Richard
M J
MJ
Robinson
D R
DR
Maresca
B
B
D002118:Calcium / Q000378:metabolism; D002455:Cell Division / Q000187:drug effects; D002460:Cell Line; D004247:DNA / Q000096:biosynthesis; D005227:Fatty Acids / Q000032:analysis / Q000494:pharmacology*; D006360:Heat-Shock Proteins / Q000096:biosynthesis*; D006358:Hot Temperature; D006801:Humans; D006861:Hydrogen Peroxide / Q000633:toxicity*; D008563:Membrane Lipids / Q000032:analysis*; D009000:Monocytes / Q000378:metabolism; D017382:Reactive Oxygen Species
D005227:Fatty Acids; D006360:Heat-Shock Proteins; D008563:Membrane Lipids; D017382:Reactive Oxygen Species; D004247:DNA; D006861:Hydrogen Peroxide; D002118:Calcium