Cycloheximide phase-shifts, but does not prevent, de novo Krox-24 protein expression.
Previous studies show that focal hippocampal injury transiently increases NMDA receptor-dependent expression of inducible transcription factors (ITFs including Krox-24) in rat dentate gyrus neurons. Furthermore, pretreatment with the protein synthesis inhibitor, cycloheximide (CHX), prevents de novo ITF protein expression 1 h post-injury. Here, we further characterize the effects of a single pretreatment dose of CHX on injury-induced expression of Krox-24 and show that CHX pretreatment phase-shifts (delays), but does not prevent, de novo expression of Krox-24 in hippocampal dentate gyrus neurons following injury. This may have implications for studies which use CHX pretreatment to examine the role of gene expression and de novo protein synthesis in long-term memory formation, the stabilization of long-term potentiation, kindling and neuronal injury.
Hughes
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PE
Research Centre for Developmental Medicine and Biology, The University of Auckland, New Zealand.
Alexi
T
T
Dragunow
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M
D000818:Animals; D003513:Cycloheximide / Q000494:pharmacology*; D004268:DNA-Binding Proteins / Q000096:biosynthesis*; D018891:Dentate Gyrus / Q000293:injuries / Q000378:metabolism / Q000473:pathology; D051766:Early Growth Response Protein 1; D006624:Hippocampus / Q000293:injuries / Q000378:metabolism / Q000473:pathology; D017874:Immediate-Early Proteins; D007150:Immunohistochemistry; D017403:In Situ Hybridization; D008297:Male; D009419:Nerve Tissue Proteins / Q000096:biosynthesis; D011500:Protein Synthesis Inhibitors / Q000494:pharmacology*; D012333:RNA, Messenger / Q000096:biosynthesis; D051381:Rats; D017208:Rats, Wistar; D014157:Transcription Factors / Q000096:biosynthesis*
D004268:DNA-Binding Proteins; D051766:Early Growth Response Protein 1; C486110:Egr1 protein, rat; D017874:Immediate-Early Proteins; D009419:Nerve Tissue Proteins; D011500:Protein Synthesis Inhibitors; D012333:RNA, Messenger; D014157:Transcription Factors; D003513:Cycloheximide