Solid phase synthesis and immunogenicity of a VP3 peptide from hepatitis A virus.
The synthesis of a peptide belonging to the VP3 capsid protein of Hepatitis A virus has been accomplished by the continuous flow Fmoc-polyamide solid phase method. The use of methoxytrimethylbenzenesulphonyl (Mtr) and pentamethylchromansulphonyl (Pmc) as arginine side-chain protecting groups in the presence of tryptophan without lateral protection or protected with t-Boc is discussed. The synthetic VP3 peptide has been administered to mice in different forms: (i) free, (ii) coupled to keyhole limpet hemocyanin, (iii) encapsulated in multilamellar (MLV) liposomes, and (iv) incorporated to a tetrameric branched lysine core. The immune response induced by these preparation is reported.
Pérez
J A
JA
Department of Peptides, CID, CSIC, Barcelona, Spain.
González-Dankaart
J F
JF
Reig
F
F
Pintó
R M
RM
Bosch
A
A
Haro
I
I
D000818:Animals; D002213:Capsid / Q000138:chemical synthesis / Q000276:immunology*; D036022:Capsid Proteins; D004339:Drug Compounding; D005260:Female; D006433:Hemocyanins / Q000276:immunology; D006508:Hepatitis Antibodies / Q000096:biosynthesis; D006507:Hepatovirus / Q000276:immunology*; D008081:Liposomes; D051379:Mice; D010446:Peptide Fragments / Q000138:chemical synthesis / Q000276:immunology*; D014611:Vaccination
D036022:Capsid Proteins; D006508:Hepatitis Antibodies; D008081:Liposomes; D010446:Peptide Fragments; C109000:VP3 peptide (110-121), hepatitis A virus; D006433:Hemocyanins; C032808:keyhole-limpet hemocyanin