title: Time differential perturbed angular correlation of 111In-EDTA linked to the single free cysteine of bovine serum albumin.
abstract: By means of a facile chemical modification of the bovine serum albumin molecule, it was possible to measure its hydrodynamic radius with high accuracy (approximately 3%) using the TDPAC technique. The new approach presented here allows a wide use of the TDPAC technique to perform high precision studies of backbone dynamics of almost any protein.
authors.affiliation: Departamento de Física, Facultad de Ciencias Exactas, Universidad Nacional de La Plata, Argentina. marcelo@venus.fisica.unlp.edu.ar
authors.forename: M
authors.initials: M
authors.lastname: Ceolín
authors.forename: J A
authors.initials: JA
authors.lastname: Martínez
authors.forename: M R
authors.initials: MR
authors.lastname: Ermácora
mesh_terms: D000818:Animals; D002417:Cattle; D055598:Chemical Phenomena; D002627:Chemistry, Physical; D003545:Cysteine / Q000737:chemistry* / Q000378:metabolism; D004492:Edetic Acid / Q000737:chemistry* / Q000378:metabolism; D007205:Indium Radioisotopes; D008968:Molecular Conformation; D012710:Serum Albumin, Bovine / Q000737:chemistry* / Q000378:metabolism; D013057:Spectrum Analysis / Q000379:methods; D013997:Time Factors
chemical_list: D007205:Indium Radioisotopes; D012710:Serum Albumin, Bovine; D004492:Edetic Acid; D003545:Cysteine