title: Monitoring the amplification of CATCH, a 3SR based cooperatively coupled isothermal amplification system, by fluorimetric methods.
abstract: Three different types of fluorescence detection methods were employed to monitor amplification of a previously established isothermal cooperatively coupled amplification system as it can serve as a tool for the investigation of fundamental issues in evolutionary optimization. By using 5'IRD-41 fluorescent labeled primers, the intercalating dye TOPRO-1 and a 5'fluorescin/3'DABCYL 4-(4-dimethylamino-phenylazo)benzoic acid labeled ss 24 nt DNA, evolving molecular cooperation is accessible, sequence specifically as well as non-sequence-specifically without using radioactivity.
authors.affiliation: Department of Molecular Information Processing, Institute of Molecular Biotechnology, Beutenbergstrasse 11, 07745 Jena, Germany. rehr@imb-jena.de
authors.forename: R
authors.initials: R
authors.lastname: Ehricht
authors.forename: T
authors.initials: T
authors.lastname: Kirner
authors.forename: T
authors.initials: T
authors.lastname: Ellinger
authors.forename: P
authors.initials: P
authors.lastname: Foerster
authors.forename: J S
authors.initials: JS
authors.lastname: McCaskill
mesh_terms: D004247:DNA / Q000032:analysis; D017931:DNA Primers; D005456:Fluorescent Dyes; D005470:Fluorometry / Q000379:methods*; D005784:Gene Amplification; D007364:Intercalating Agents; D009690:Nucleic Acid Conformation; D017422:Sequence Analysis, DNA; D004124:p-Dimethylaminoazobenzene / Q000031:analogs & derivatives
chemical_list: D017931:DNA Primers; D005456:Fluorescent Dyes; D007364:Intercalating Agents; C063127:4-(4-dimethylaminophenylazo)benzoic acid; D004247:DNA; D004124:p-Dimethylaminoazobenzene