Map-based cloning of a gene sequence encoding a nucleotide-binding domain and a leucine-rich region at the Cre3 nematode resistance locus of wheat.
The Cre3 gene confers a high level of resistance to the root endoparasitic nematode Heterodera avenae in wheat. A DNA marker cosegregating with H. avenae resistance was used as an entry point for map-based cloning of a disease resistance gene family at the Cre3 locus. Two related gene sequences have been analysed at the Cre3 locus. One, identified as a cDNA clone, encodes a polypeptide with a nucleotide binding site (NBS) and a leucine-rich region; this member of the disease resistance gene family is expressed in roots. A second Cre3 gene sequence, cloned as genomic DNA, appears to be a pseudogene, with a frame shift caused by a deletion event. These two genes, related to members of the cytoplasmic NBS-leucine rich repeat class of plant disease resistance genes were physically mapped to the distal 0.06 fragment of the long arm of wheat chromosome 2D and cosegregated with nematode resistance.
Lagudah
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Commonwealth Scientific and Industrial Research Organization, Division of Plant Industry, Canberra, Australia. e.lagudah@pi.csiro.au
Moullet
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D000595:Amino Acid Sequence; D000818:Animals; D001665:Binding Sites / Q000235:genetics; D002874:Chromosome Mapping; D003001:Cloning, Molecular; D007930:Leucine / Q000235:genetics*; D008969:Molecular Sequence Data; D009348:Nematoda / Q000472:pathogenicity*; D010935:Plant Diseases / Q000235:genetics* / Q000469:parasitology; D010940:Plant Proteins / Q000235:genetics*; D018517:Plant Roots / Q000469:parasitology; D015183:Restriction Mapping; D016415:Sequence Alignment; D014908:Triticum / Q000235:genetics* / Q000276:immunology
C109943:Cre3 protein, Triticum aestivum; D010940:Plant Proteins; D007930:Leucine