Sialic acid measurement by a modified Aminoff method: a time-saving reduction in 2-thiobarbituric acid concentration.
Our results show that if the 2-thiobarbituric acid concentration is decreased, its co-precipitation with the chromophore is diminished. Subsequent running of this reaction mixture by high-performance liquid chromatography still allows measurement of Neu-5-Ac in the picomole order, with a substantial time and reactive saving, as compared with the original assay.
Romero
E L
EL
Departamento de Ciencia y Tecnología, Universidad Nacional de Quilmes, Buenos Aires, Argentina.
Pardo
M F
MF
Porro
S
S
Alonso
S
S
D011232:Chemical Precipitation; D002623:Chemistry Techniques, Analytical / Q000379:methods*; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D006863:Hydrogen-Ion Concentration; D019158:N-Acetylneuraminic Acid / Q000032:analysis*; D012680:Sensitivity and Specificity; D013858:Thiobarbiturates
D013858:Thiobarbiturates; D019158:N-Acetylneuraminic Acid; C029684:thiobarbituric acid