Antifungal susceptibility testing of yeast isolates from blood cultures by microbroth dilution and the E test.
The results of microbroth dilution were compared with those of the E test for 169 yeast isolates tested for their susceptibility to antifungal agents. All isolates were tested by both methods against amphotericin B, ketoconazole, fluconazole, and itraconazole. The E test results generally correlated well with those obtained by the reference method. There was at least 80% agreement of minimum inhibitory concentration results within two dilutions for all yeast species and agents tested, except for Cryptococcus neoformans tested with fluconazole (8% agreement). The E test appears to be a suitable alternative antifungal susceptibility test method for yeasts, although improvements are required for testing Cryptococcus neoformans against fluconazole.
Simor
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AE
Department of Microbiology, Sunnybrook Health Science Centre, University of Toronto, Ontario, Canada.
Goswell
G
G
Louie
L
L
Lee
M
M
Louie
M
M
D000935:Antifungal Agents / Q000494:pharmacology*; D002175:Candida / Q000187:drug effects* / Q000254:growth & development; D003455:Cryptococcus neoformans / Q000187:drug effects*; D003470:Culture Media; D006801:Humans; D008826:Microbial Sensitivity Tests
D000935:Antifungal Agents; D003470:Culture Media