title: Studies of AIDS vaccination using an ex vivo feline immunodeficiency virus model: protection conferred by a fixed-cell vaccine against cell-free and cell-associated challenge differs in duration and is not easily boosted.
abstract: Cats immunized with cells infected with a primary isolate of feline immunodeficiency virus (FIV) and fixed with paraformaldehyde were challenged with cell-free or cell-associated homologous virus obtained ex vivo. Complete protection was observed in animals challenged with cell-free virus 4 months after completion of vaccination (p.v.) or with cell-associated virus 12 months p.v. In contrast, no protection was observed in cats challenged with cell-free virus 12 or 28 months p.v. or with cell-associated virus 37.5 months p.v. Prior to the 28- and 37.5-month challenges, the animals had received a booster dose of vaccine that had elicited a robust anamnestic immune response. These results show that vaccine-induced protection against ex vivo FIV is achievable but is relatively short-lived and can be difficult to boost.
authors.affiliation: Department of Biomedicine, University of Pisa, Italy.
authors.forename: D
authors.initials: D
authors.lastname: Matteucci
authors.forename: M
authors.initials: M
authors.lastname: Pistello
authors.forename: P
authors.initials: P
authors.lastname: Mazzetti
authors.forename: S
authors.initials: S
authors.lastname: Giannecchini
authors.forename: D
authors.initials: D
authors.lastname: Del Mauro
authors.forename: I
authors.initials: I
authors.lastname: Lonetti
authors.forename: L
authors.initials: L
authors.lastname: Zaccaro
authors.forename: C
authors.initials: C
authors.lastname: Pollera
authors.forename: S
authors.initials: S
authors.lastname: Specter
authors.forename: M
authors.initials: M
authors.lastname: Bendinelli
mesh_terms: D016915:AIDS Vaccines / Q000276:immunology*; D000818:Animals; D000956:Antigens, Viral / Q000276:immunology; D002415:Cats; D002474:Cell-Free System; D004195:Disease Models, Animal; D005260:Female; D007117:Immunization, Secondary; D016089:Immunodeficiency Virus, Feline / Q000276:immunology*; D007156:Immunologic Memory; D012367:RNA, Viral / Q000032:analysis; D013997:Time Factors; D014611:Vaccination
chemical_list: D016915:AIDS Vaccines; D000956:Antigens, Viral; D012367:RNA, Viral