title: Chromosome assignment of aberrant NotI restriction DNA fragments in primary hepatocellular carcinoma.
abstract: DNA aberrations in human hepatocellular carcinoma (HCC) were studied by two-dimensional DNA electrophoresis analysis. Five intensified and 60 dwindling spots were detected recurrently in the two-dimensional profile which showed about 3000 restriction DNA fragments as distinctive spots. We assigned these aberrant spots to chromosomes, using the chromosome-assigned two-dimensional profile. Four of the five intensified, and 53 of the 60 dwindling spots were given chromosome assignments. Intensified spots were assigned to chromosomes 5, 6, 9 through 12, 16 and 18. Among the dwindling spots, the highest incidence of aberrations was found on chromosome 16, followed by 9 through 12 and chromosome 2. No aberrations were detected in chromosomes 7, 21, 22 or Y.
authors.affiliation: Institute for Molecular and Cellular Biology, Osaka University, Japan.
authors.forename: H
authors.initials: H
authors.lastname: Yoshikawa
authors.forename: H
authors.initials: H
authors.lastname: Nagai
authors.forename: K S
authors.initials: KS
authors.lastname: Oh
authors.forename: S
authors.initials: S
authors.lastname: Tamai
authors.forename: A
authors.initials: A
authors.lastname: Fujiyama
authors.forename: T
authors.initials: T
authors.lastname: Nakanishi
authors.forename: G
authors.initials: G
authors.lastname: Kajiyama
authors.forename: K
authors.initials: K
authors.lastname: Matsubara
mesh_terms: D006528:Carcinoma, Hepatocellular / Q000235:genetics*; D002869:Chromosome Aberrations / Q000235:genetics*; D002874:Chromosome Mapping / Q000379:methods*; D002877:Chromosomes, Human / Q000235:genetics; D004273:DNA, Neoplasm / Q000032:analysis* / Q000235:genetics; D015252:Deoxyribonucleases, Type II Site-Specific; D015180:Electrophoresis, Gel, Two-Dimensional / Q000379:methods; D006801:Humans; D008113:Liver Neoplasms / Q000235:genetics*; D019656:Loss of Heterozygosity; D009154:Mutation
chemical_list: D004273:DNA, Neoplasm; D015252:Deoxyribonucleases, Type II Site-Specific; C038565:GCGGCCGC-specific type II deoxyribonucleases