Detection of malignant cells of epithelial origin in mononuclear cell fractions.
Qualitative and quantitative detection of contaminating malignant cells in stem cell apheresis products intended for supportive therapy after high-dose chemotherapy of sensitive solid tumors is a demanding task for the laboratory. Human breast cancer cells in differing concentrations among mononuclear cells were quantitated by multicolor flow cytometry and immunocytochemistry. Comparing specificity, sensitivity, and laboratory processing time, the immunocytochemical detection of the above-mentioned malignant cells of epithelial origin was demonstrated to be the superior method.
Gebauer
W
W
Red Cross Blood Transfusion Service Oldenburg, Germany.
Fallgren Gebauer
E
E
Corbes
C
C
Janssen
S
S
Schunter
F
F
D001943:Breast Neoplasms / Q000097:blood / Q000628:therapy*; D002452:Cell Count; D004847:Epithelial Cells; D005260:Female; D005434:Flow Cytometry; D018380:Hematopoietic Stem Cell Transplantation; D006801:Humans; D007150:Immunohistochemistry; D009000:Monocytes; D014407:Tumor Cells, Cultured