Polypeptide composition of submitochondrial fractions of normal liver tissue and Zajdela hepatoma.
The polypeptide composition of liver and Zajdela hepatoma mitochondria is compared. Polypeptides of mitochondria and submitochondrial fractions (the outer and the inner mitochondrial membranes, the intermembrane and the matrix spaces of mitochondria) were separated by electrophoresis in polyacrylamide gel. The percentage content of each polypeptide was evaluated after scanning gels using a differential spectrophotometer-densitometer. Significant changes of several proteins of the hepatoma mitochondria and submitochondrial fractions as compared with normal liver preparations have been found.
Azarashvili
T S
TS
Institute of Theoretical and Experimental Biophysics, Russian Academy of Sciences, Pushchino, Moscow Region, Russia.
Kudin
A P
AP
Polteva
N A
NA
Kudzina LYu
Evtodienko YuV
D000818:Animals; D008114:Liver Neoplasms, Experimental / Q000737:chemistry*; D008297:Male; D008930:Mitochondria, Liver / Q000737:chemistry*; D008970:Molecular Weight; D009363:Neoplasm Proteins / Q000737:chemistry* / Q000302:isolation & purification; D010455:Peptides / Q000737:chemistry* / Q000302:isolation & purification; D051381:Rats; D017208:Rats, Wistar; D013367:Submitochondrial Particles / Q000737:chemistry
D009363:Neoplasm Proteins; D010455:Peptides