Determination of p-aminosalicylic acid and its N-acetylated metabolite in human urine by capillary zone electrophoresis as a measure of in vivo N-acetyltransferase 1 activity.
A capillary zone electrophoresis method has been developed for the determination of p-aminosalicylic acid (PAS) and its metabolite, N-acetyl-p-aminosalicylic acid (N-acetyl-PAS), in urine. A linear relationship was observed between time-normalized peak area and the concentration of the parent and metabolite with correlation coefficients greater than 0.9990. The method could be applied to the determination of PAS and N-acetyl-PAS in human urine without any sample pretreatment. A good separation of the analytes is achieved in a run time of 12 min (15 min total, including capillary wash). Using PAS as a probe for N-acetyltransferase 1 activity, 20 healthy volunteers were phenotyped after oral administration of a 1 g dose. The preliminary results seem to indicate a bimodal distribution of N-acetyl-PAS/PAS molar ratios.
Cummins
C L
CL
Department of Chemistry, McGill University, Montreal, Quebec, Canada.
O'Neil
W M
WM
Soo
E C
EC
Lloyd
D K
DK
Wainer
I W
IW
D000081:Acetamides; D000293:Adolescent; D000328:Adult; D010131:Aminosalicylic Acid / Q000652:urine*; D000636:Aminosalicylic Acids / Q000652:urine*; D001191:Arylamine N-Acetyltransferase / Q000235:genetics / Q000652:urine*; D019075:Electrophoresis, Capillary; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D010641:Phenotype; D012459:Salicylates / Q000652:urine*
D000081:Acetamides; D000636:Aminosalicylic Acids; D012459:Salicylates; C086759:acetyl 4-aminosalicylic acid; C055814:4-acetamidosalicylic acid; D010131:Aminosalicylic Acid; D001191:Arylamine N-Acetyltransferase