title: Novel method for stereo imaging in light microscopy at high magnifications.
abstract: A new method for realizing direct stereoscopic (3D) views of thick microscopic sections employs multiple oblique illuminating beams and a single objective lens. Excellent 3D images are obtained in the higher magnification range, where conventional stereo microscopes no longer function. Using conventional microscope optics, significant increases in depth of focus and sharpness are demonstrated.
authors.affiliation: Department of Anatomy and Developmental Biology, University College, London, United Kingdom.
authors.forename: G
authors.initials: G
authors.lastname: Greenberg
authors.forename: A
authors.initials: A
authors.lastname: Boyde
mesh_terms: D000544:Alzheimer Disease / Q000473:pathology; D000818:Animals; D002531:Cerebellum / Q000473:pathology; D017377:Diatoms / Q000648:ultrastructure; D006801:Humans; D007091:Image Processing, Computer-Assisted / Q000295:instrumentation*; D008853:Microscopy / Q000295:instrumentation*; D009045:Motor Endplate / Q000473:pathology; D019579:Neocortex / Q000473:pathology; D017072:Neostriatum / Q000473:pathology; D009420:Nervous System / Q000473:pathology*; D055095:Optics and Photonics; D010780:Photogrammetry / Q000295:instrumentation; D017966:Pyramidal Cells / Q000648:ultrastructure