Novel selective and non-selective optical detection of microorganisms.
A new instrument, capable of detecting metabolic changes due to microbiological activity, is described. Optical changes in growth media are monitored in a semi-fluid zone that separates the liquid medium containing the sample. Data demonstrate that common media can be utilized in conjunction with this rapid automated technology. Nutrient broth with the pH dye indicator. bromocresol purple was suitable for total counts. Selective media containing dyes were utilized to assess the presence or absence of specific groups of organisms. Biochemical reactions, such as lysine decarboxylase activity, were identified by the unique generated patterns, and specific enzymatic cleavage reactions with chromogenic substrates, such as 5-bromo-4 chloro-3 indolyl-beta-D-glucuronic acid (X-GLUC), were monitored.
Shelef
L A
LA
Department of Nutrition and Food Science, Wayne State University, Detroit, MI, USA.
Firstenberg-Eden
R
R
D001409:Bacillus cereus / Q000254:growth & development / Q000302:isolation & purification / Q000378:metabolism; D001962:Bromcresol Purple; D002262:Carboxy-Lyases / Q000378:metabolism; D002863:Chromogenic Compounds; D003470:Culture Media; D004926:Escherichia coli / Q000254:growth & development / Q000302:isolation & purification / Q000378:metabolism; D005069:Evaluation Studies as Topic; D005516:Food Microbiology; D005960:Glucosides; D006863:Hydrogen-Ion Concentration; D007202:Indicators and Reagents; D007211:Indoles; D008239:Lysine / Q000378:metabolism; D008828:Microbiological Techniques / Q000295:instrumentation*; D055095:Optics and Photonics / Q000295:instrumentation*; D011513:Proteus mirabilis / Q000254:growth & development / Q000302:isolation & purification / Q000378:metabolism; D012477:Salmonella enteritidis / Q000254:growth & development / Q000302:isolation & purification / Q000378:metabolism; D013211:Staphylococcus aureus / Q000254:growth & development / Q000302:isolation & purification / Q000378:metabolism
D002863:Chromogenic Compounds; D003470:Culture Media; D005960:Glucosides; D007202:Indicators and Reagents; D007211:Indoles; C052409:5-bromo-4-chloro-3-indoxylglucoside; D001962:Bromcresol Purple; D002262:Carboxy-Lyases; C021520:lysine decarboxylase; D008239:Lysine