title: A simplified sequence for observing deoxymyoglobin signals in vivo: myoglobin excitation with dynamic unexcitation and saturation of water and fat (MEDUSA).
abstract: This paper describes a new, simplified pulse sequence for observing NMR signals from deoxymyoglobin in vivo. Paramagnetically shifted resonances from deoxymyoglobin can be exploited to noninvasively calculate intracellular oxygen tension in striated muscle. However, special sequences are required to observe these weak signals against the larger water and fat signals encountered in vivo. The pulse sequence described here, which is based on inversion recovery sequences, efficiently suppresses both water and fat resonances and can be implemented with short repetition rates. Moreover, it is perfectly suited for studies with surface coils, where RF inhomogeneities render other popular suppression sequences ineffective.
authors.affiliation: MMRRCC, Department of Radiology, University of Pennsylvania, Philadelphia 19104-6100, USA.
authors.forename: E A
authors.initials: EA
authors.lastname: Noyszewski
authors.forename: E L
authors.initials: EL
authors.lastname: Chen
authors.forename: R
authors.initials: R
authors.lastname: Reddy
authors.forename: Z
authors.initials: Z
authors.lastname: Wang
authors.forename: J S
authors.initials: JS
authors.lastname: Leigh
mesh_terms: D006801:Humans; D009682:Magnetic Resonance Spectroscopy / Q000379:methods*; D018482:Muscle, Skeletal / Q000378:metabolism*; D009211:Myoglobin / Q000031:analogs & derivatives* / Q000032:analysis
chemical_list: D009211:Myoglobin; C055547:deoxymyoglobin