title: Elimination of 1-hydroxypyrene after human volunteer exposure to polycyclic aromatic hydrocarbons.
abstract: The aim of this study was to estimate the kinetics of 1-hydroxypyrene (1-HP) elimination after inhalation exposure to polycyclic aromatic hydrocarbons (PAHs). Samples of inhaled and exhaled air were collected on glass fiber filters backed with tubes filled with Amberlit XAD-2 resin. The filters were extracted by cyclohexane and Amberlit--by acetonitrile. Extracts for the determination of pyrene and benzo[a]pyrene (B[a]P) concentrations were analyzed by high-performance liquid chromatography (HPLC). 1-Hydroxypyrene in urine was determined after its preconcentration on a C-18 column (solid phase extraction method) using the same analytical technique. Five male volunteers were exposed for 6 h (two times, with a 1-month interval) to a PAH mixture at an aluminium plant. The volunteers were breathing at rest through facial mask equipped with a 1000-ml compensation container which allows collection of the exhaled air. Inhaled air samples were collected in the breathing zone of each volunteer. Urine samples were collected until the 71st hour after the onset of exposure. The average respiratory retention of pyrene was found to be 61%. The 1-HP elimination process could be described by one-compartment model with the half-live of 9.8 hour (95% CI 7.9-11.7 h). The simulation of 1-HP elimination in urine during a working week (4 days) indicates that the balance between absorption and elimination is achieved at the end of the second day.
authors.affiliation: Nofer Institute of Occupational Medicine, Lódź, Poland.
authors.forename: S
authors.initials: S
authors.lastname: Brzeźnicki
authors.forename: M
authors.initials: M
authors.lastname: Jakubowski
authors.forename: B
authors.initials: B
authors.lastname: Czerski
mesh_terms: D000280:Administration, Inhalation; D000328:Adult; D000395:Air Pollutants, Occupational / Q000493:pharmacokinetics* / Q000633:toxicity; D004784:Environmental Monitoring; D006207:Half-Life; D006801:Humans; D008297:Male; D008657:Metabolic Clearance Rate / Q000502:physiology; D011084:Polycyclic Aromatic Hydrocarbons / Q000493:pharmacokinetics* / Q000633:toxicity; D011721:Pyrenes / Q000493:pharmacokinetics*
chemical_list: D000395:Air Pollutants, Occupational; D011084:Polycyclic Aromatic Hydrocarbons; D011721:Pyrenes; C033146:1-hydroxypyrene