title: The clinical value of cathepsin-D and TNF-alpha in bladder cancer patients.
abstract: This study included 34 normal healthy controls, 35 patients with urinary tract bilharziasis and 93 bladder cancer patients (62 were operable cases and 31 non-operable). Serum tumor necrosis factor alpha (TNF-alpha) was determined using the enzyme immunoassay reagents supplied by Medgenix Diagnostics, Belgium. Cytosol Cathepsin-D was estimated using the immunoradiometric assay supplied by CIS BIO International, France. The results revealed that at 100% and 90% specificities, cytosol Cathepsin-D had 35.7% and 59.5% sensitivity in bladder cancer patients. Serum TNF-alpha showed sensitivity of 17.0% and 55.0% at 100% and 90% specificities in operable bladder cancer patients and 48.0% and 77.0% in non-operable cases respectively. Cytosol cathepsin D and TNF-alpha did not show prognostic values like positive correlation with tumor stages, grades or association of tumors with bilharzial ova or lymph node involvement.
authors.affiliation: Tumor Marker Oncology Research Center, Al-Azhar University, Egypt.
authors.forename: T
authors.initials: T
authors.lastname: Salman
authors.forename: O
authors.initials: O
authors.lastname: el-Ahmady
authors.forename: M
authors.initials: M
authors.lastname: el-Shafee
authors.forename: S
authors.initials: S
authors.lastname: Omar
authors.forename: I
authors.initials: I
authors.lastname: Salman
mesh_terms: D000328:Adult; D000368:Aged; D014408:Biomarkers, Tumor / Q000032:analysis*; D002402:Cathepsin D / Q000032:analysis*; D003600:Cytosol / Q000201:enzymology; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D012552:Schistosomiasis / Q000378:metabolism; D012680:Sensitivity and Specificity; D014409:Tumor Necrosis Factor-alpha / Q000032:analysis*; D001749:Urinary Bladder Neoplasms / Q000737:chemistry*
chemical_list: D014408:Biomarkers, Tumor; D014409:Tumor Necrosis Factor-alpha; D002402:Cathepsin D