Increases in hematopoietic responses caused by beta-glucans in mice.
The effects of various (1-->3)-beta-D-glucans on hematopoietic responses of mice were investigated by measuring colony stimulating activity in sera and ascites of the mice administered glucan. We have demonstrated that the hematopoietic response was increased by various structures of (1-->3)-beta-D-glucans, i.e. soluble glucans (linear, branched, single helix, triple helix) and particulate glucans. From the viewpoint of structure and activity relationships, we found several characteristic features: i) hematopoietic response induced by the particulate glucan disappeared faster than that by the soluble glucans, ii) conformation of the glucans, single vs. triple helix, are relatively independent of the response, iii) linear glucan had a weaker response, and iv) there is a strong strain-dependency of the response. These results corresponded well with the fact that branched (1-->3)-beta-D-glucans, but not linear and not particulate, are often used as biological response modifiers for cancer patients.
Tateishi
T
T
Laboratory of Immunopharmacology of Microbial Products, School of Pharmacy, Tokyo University of Pharmacy and Life Science, Japan.
Ohno
N
N
Adachi
Y
Y
Yadomae
T
T
D000818:Animals; D001201:Ascites / Q000378:metabolism; D003115:Colony-Stimulating Factors / Q000494:pharmacology; D005070:Evans Blue; D005936:Glucans / Q000737:chemistry / Q000494:pharmacology*; D006410:Hematopoiesis / Q000187:drug effects*; D007155:Immunologic Factors / Q000737:chemistry / Q000494:pharmacology*; D007274:Injections, Intraperitoneal; D007375:Interleukin-1 / Q000096:biosynthesis; D015850:Interleukin-6 / Q000096:biosynthesis; D008264:Macrophages / Q000187:drug effects / Q000378:metabolism; D051379:Mice; D008810:Mice, Inbred C57BL; D008813:Mice, Inbred ICR; D016513:Mice, SCID; D009569:Nitric Oxide / Q000096:biosynthesis; D012972:Sodium Hydroxide / Q000494:pharmacology; D013329:Structure-Activity Relationship
D003115:Colony-Stimulating Factors; D005936:Glucans; D007155:Immunologic Factors; D007375:Interleukin-1; D015850:Interleukin-6; C084912:Zymocel; D009569:Nitric Oxide; D005070:Evans Blue; D012972:Sodium Hydroxide