Differential stimulation requirements for IL-12 production among clones of macrophage hybridomas.
We have previously established cloned macrophage hybridomas by somatic cell fusion of the macrophage tumor P388D1 of DBA/2 (H-2d) origin with splenic adherent cells of CKB mice (H-2k). Several cloned lines displayed the serologic and functional characteristics of macrophages. In this study, we evaluated the ability of these hybridomas to produce IL-12 after combined stimulation with IFN-gamma and lipopolysaccharide (LPS). The patterns of IL-12 production by these cloned macrophages fell into three groups. The first group produced IL-12 on stimulation with LPS in combination with IFN-gamma pretreatment, the second group produced IL-12 on stimulation with LPS regardless of the pretreatment with IFN-gamma, and the third group did not produce IL-12 at all on stimulation with IFN-gamma and LPS. None of the macrophage clones tested produced IL-12 constitutively. The results correlated well with IL-12 p40 mRNA expression in those macrophages as detected by RT-PCR. These results suggest the differential stimulation requirements for IL-12 production among macrophages at a clonal level.
Taneichi
M
M
Department of Bacterial and Blood Products, National Institute of Infectious Diseases, Tokyo, Japan.
Horino
A
A
Naito
S
S
Sakurai
S
S
Komuro
K
K
Uchida
T
T
D000818:Animals; D002999:Clone Cells; D000949:Histocompatibility Antigens Class II / Q000097:blood; D006825:Hybridomas / Q000378:metabolism*; D018664:Interleukin-12 / Q000096:biosynthesis*; D008070:Lipopolysaccharides / Q000494:pharmacology; D008264:Macrophages / Q000378:metabolism*; D051379:Mice; D013268:Stimulation, Chemical
D000949:Histocompatibility Antigens Class II; D008070:Lipopolysaccharides; D018664:Interleukin-12