Requirements for Epstein-Barr nuclear antigen 1 (EBNA1)-induced permanganate sensitivity of the epstein-barr virus latent origin of DNA replication.
Epstein-Barr nuclear antigen 1 (EBNA1) activates DNA replication from the Epstein-Barr virus latent origin of DNA replication, oriP. EBNA1 binds cooperatively to four recognition sites in the dyad symmetry (DS) element of oriP, causing alterations in the origin DNA structure, which can be detected by the increased sensitivity of one Thy residue in two of the binding sites to permanganate oxidation. To better understand the significance of this EBNA1-induced origin distortion, we have investigated the DNA sequence and EBNA1 amino acid requirements for permanganate sensitivity. We have shown that the EBNA1 DNA binding and dimerization domains are sufficient to induce permanganate sensitivity and that amino acids 463-467, which form an extended chain that travels along the minor groove of the EBNA1 recognition site, play an important role in generating the DNA distortion. The EBNA1-induced permanganate sensitivity is independent of cooperative interactions between EBNA1 molecules on the origin and requires a specific sequence within the EBNA1 binding site. Using synthetic EBNA1 binding sites, we found that the inversion of a single AT base pair in the EBNA1 recognition sequence is sufficient to confer EBNA1-induced permanganate sensitivity. These studies indicate that permanganate oxidation can detect very minor alterations in DNA structure.
Summers
H
H
Cancer Research Group, Institute for Molecular Biology and Biotechnology, McMaster University, Hamilton, Ontario, Canada L8N 3Z5.
Fleming
A
A
Frappier
L
L
D001665:Binding Sites; D004279:DNA, Viral / Q000737:chemistry*; D019309:Epstein-Barr Virus Nuclear Antigens / Q000378:metabolism / Q000502:physiology*; D017895:Manganese Compounds / Q000494:pharmacology*; D009690:Nucleic Acid Conformation; D010087:Oxides / Q000494:pharmacology*; D018741:Replication Origin; D017735:Virus Latency / Q000235:genetics*
D004279:DNA, Viral; D019309:Epstein-Barr Virus Nuclear Antigens; D017895:Manganese Compounds; D010087:Oxides; C048856:permanganic acid