A simple quantitative measurement of mRNA of human beta-actin by reverse transcription competitive PCR with a compact digital camera.
Reverse transcription (RT) competitive polymerase chain reaction (PCR) is a sensitive and useful technique for the absolute quantitation of mRNA level. We developed a competitive PCR method with a compact digital camera DC 40 (Eastman Kodak Company, NY, U.S.A.) for the measurement of human beta-actin cDNA (mRNA), a reference gene in an analysis for target gene expression. The devised method, compared with currently used methods, was easy, safe, sensitive, and highly reproducible to assay an infinitesimal level of cDNA (mRNA).
Okamoto
Y
Y
Department of Clinical Pharmacy, Faculty of Pharmacy, Meijo Unviersity, Nagoya, Japan.
Hara
Y
Y
Katoh
Y
Y
Nagai
H
H
Nishida
M
M
D000199:Actins / Q000235:genetics*; D001667:Binding, Competitive; D006801:Humans; D016014:Linear Models; D010781:Photography / Q000295:instrumentation*; D016133:Polymerase Chain Reaction / Q000379:methods*; D012333:RNA, Messenger / Q000032:analysis*; D015203:Reproducibility of Results; D012815:Signal Processing, Computer-Assisted; D014158:Transcription, Genetic
D000199:Actins; D012333:RNA, Messenger