Mechanical separation of the complementary strands of DNA.
We describe the mechanical separation of the two complementary strands of a single molecule of bacteriophage lambda DNA. The 3' and 5' extremities on one end of the molecule are pulled progressively apart, and this leads to the opening of the double helix. The typical forces along the opening are in the range of 10-15 pN. The separation force signal is shown to be related to the local GC vs. AT content along the molecule. Variations of this content on a typical scale of 100-500 bases are presently detected.
Essevaz-Roulet
B
B
Laboratoire de Physique de la Matière Condensée, URA 1437, Ecole Normale Supérieure, 24 rue Lhomond, 75005 Paris, France.
Bockelmann
U
U
Heslot
F
F
D010582:Bacteriophage lambda; D001482:Base Composition; D055592:Biophysical Phenomena; D001703:Biophysics; D004277:DNA, Single-Stranded / Q000302:isolation & purification*; D004279:DNA, Viral; D008846:Micromanipulation / Q000379:methods*; D018625:Microscopy, Atomic Force; D009691:Nucleic Acid Denaturation; D055095:Optics and Photonics; D012399:Rotation; D013816:Thermodynamics
D004277:DNA, Single-Stranded; D004279:DNA, Viral