Genetic variation in two cultures of Bradyrhizobium japonicum 110 differing in their ability to impart drought tolerance to soybean.
The polymerase chain reaction with arbitrary primers (RAPD) discriminated between two separately maintained cultures of Bradyrhizobium japonicum USDA 110 differing in symbiotic performance under drought conditions. Since strain 110 is used in inoculum production, the use of RAPD to monitor inoculum cultures could help to preserve their genetic composition and prevent the loss of important symbiotic properties. The use of RAPD could also be extended to other B. japonicum strains currently used in inoculum production.
Mathis
J N
JN
Department of Biology, State University of West Georgia, Carrollton 30118, USA.
McMillin
D E
DE
Champion
R A
RA
Hunt
P G
PG
D014644:Genetic Variation; D008353:Mannitol / Q000378:metabolism; D019105:Random Amplified Polymorphic DNA Technique; D012231:Rhizobium / Q000235:genetics*; D013025:Glycine max / Q000382:microbiology*
D008353:Mannitol