GABA and glutamate levels in the substantia nigra reticulata following repetitive cerebral ischemia in gerbils.
Repetitive cerebral ischemia produces more severe damage than a similar single duration insult. We have previously shown that, in gerbils, damage in the substantia nigra reticulata (SNr) is seen with repetitive insults rather than a single insult. We have also shown that there is a progressive decrease in the extracellular GABA in the striatum in the days preceding such damage, speculating that a loss of GABA may be in part responsible for this damage. This study evaluates the GABA levels in the SNr in animals exposed to repetitive ischemic insults. Each animal received a total of three ischemic insults of 3-min duration at hourly intervals. In vivo microdialysis was carried out to analyze the GABA and glutamate dialysate levels on Days 1, 3, 5, 7, and 14 following the ischemic insult. In the control and treated (ischemic) animals, there was a significant increase in the GABA levels with the introduction of nipecotic acid on Days 1, 3, 5, and 14. However, on Day 7 there was a significant attenuation in the GABA response to nipecotic acid in the treated animals in comparison to the controls. The glutamate levels in the treated animals were similar to the control animals on Days 1, 3, 5, and 7. However, on Day 14 the glutamate levels were significantly lower than on previous days. Our experiments for the first time measure extracellular glutamate and GABA responses in the SNr in animals exposed to repetitive ischemic insults. Our experiments show that there is a significant decrease in the GABA concentrations at a time when ischemic damage is developing in this region. This confirms our hypothesis that a decrease in GABA may be one factor contributing to neuronal damage during the period following repetitive ischemic insults. Further, the rebound increase in GABA levels on Day 14 with a concomitant fall in glutamate levels would indicate that reparative processes are still active in the 2 weeks following the insult.
Shuaib
A
A
Department of Medicine, and Saskatchewan Stroke Research Centre, University of Saskatchewan, Saskatoon, Canada.
Ijaz
M S
MS
Miyashita
H
H
Hussain
S
S
Kanthan
R
R
D000818:Animals; D001925:Brain Damage, Chronic / Q000209:etiology / Q000473:pathology; D002545:Brain Ischemia / Q000150:complications / Q000378:metabolism* / Q000473:pathology; D002851:Chromatography, High Pressure Liquid; D003289:Convalescence; D005849:Gerbillinae; D018698:Glutamic Acid / Q000032:analysis*; D008297:Male; D017551:Microdialysis; D012008:Recurrence; D013378:Substantia Nigra / Q000737:chemistry*; D013997:Time Factors; D005680:gamma-Aminobutyric Acid / Q000032:analysis*
D018698:Glutamic Acid; D005680:gamma-Aminobutyric Acid