title: Interleukin-1 in ischemia-reperfusion acute lung injury.
abstract: Interleukin-1 (IL-1) is a proinflammatory cytokine produced by blood-borne and resident inflammatory lung tissue involved in the thrombotic occlusion of the pulmonary microcirculation and the increase of the vascular permeability following a wide variety of injuries and sepsis. The locally accentuated, organ-related activation of this cytokine seems to be responsible for the development of acute lung injury. The present study was conducted to determine if IL-1beta was produced in an ischemia-reperfusion (I/R) rat model subjected to lung injury. We measured sequential perfusate levels of IL-1beta by ELISA and we measured IL-1 gene expression in the rat lung tissue by a reverse-transcriptase polymerase chain reaction method. Little IL-1beta gene expression was observed in normal rat lung tissue. Perfusate IL-1beta slightly increased 2 h after induced ischemia and 3 h after reperfusion. IL-1beta gene expression rapidly increased as early as 30 min after ischemia and continued to increase for up to 120 min. IL-1beta gene expression was dramatically upregulated during reperfusion after cessation of ischemia, reached a peak at 1 h, and then gradually decreased (2 to 3 h) to near baseline levels. During ischemia, the increased IL-1 gene expression was not significantly different between the ventral and dorsal sites of the lung. However, IL-1 gene expression markedly increased on the dorsal part (the dependent site for a rat in a supine position) after reperfusion. From these results, it appears that IL-1 may have an important role in I/R lung injury.
authors.affiliation: Department of Medicine, Tri-Service General Hospital, Taipei, Taiwan, Republic of China.
authors.forename: D M
authors.initials: DM
authors.lastname: Chang
authors.forename: K
authors.initials: K
authors.lastname: Hsu
authors.forename: Y A
authors.initials: YA
authors.lastname: Ding
authors.forename: C H
authors.initials: CH
authors.lastname: Chiang
mesh_terms: D000208:Acute Disease; D000818:Animals; D002199:Capillary Permeability; D017931:DNA Primers / Q000737:chemistry; D004195:Disease Models, Animal; D004797:Enzyme-Linked Immunosorbent Assay; D066298:In Vitro Techniques; D007375:Interleukin-1 / Q000235:genetics / Q000378:metabolism*; D008171:Lung Diseases / Q000378:metabolism*; D008297:Male; D016133:Polymerase Chain Reaction; D012313:RNA / Q000096:biosynthesis; D051381:Rats; D017207:Rats, Sprague-Dawley; D015427:Reperfusion Injury / Q000378:metabolism*
chemical_list: D017931:DNA Primers; D007375:Interleukin-1; D012313:RNA