title: Antifungal susceptibility testing of yeast isolates from blood cultures by microbroth dilution and the E test.
abstract: The results of microbroth dilution were compared with those of the E test for 169 yeast isolates tested for their susceptibility to antifungal agents. All isolates were tested by both methods against amphotericin B, ketoconazole, fluconazole, and itraconazole. The E test results generally correlated well with those obtained by the reference method. There was at least 80% agreement of minimum inhibitory concentration results within two dilutions for all yeast species and agents tested, except for Cryptococcus neoformans tested with fluconazole (8% agreement). The E test appears to be a suitable alternative antifungal susceptibility test method for yeasts, although improvements are required for testing Cryptococcus neoformans against fluconazole.
authors.affiliation: Department of Microbiology, Sunnybrook Health Science Centre, University of Toronto, Ontario, Canada.
authors.forename: A E
authors.initials: AE
authors.lastname: Simor
authors.forename: G
authors.initials: G
authors.lastname: Goswell
authors.forename: L
authors.initials: L
authors.lastname: Louie
authors.forename: M
authors.initials: M
authors.lastname: Lee
authors.forename: M
authors.initials: M
authors.lastname: Louie
mesh_terms: D000935:Antifungal Agents / Q000494:pharmacology*; D002175:Candida / Q000187:drug effects* / Q000254:growth & development; D003455:Cryptococcus neoformans / Q000187:drug effects*; D003470:Culture Media; D006801:Humans; D008826:Microbial Sensitivity Tests
chemical_list: D000935:Antifungal Agents; D003470:Culture Media