An isolated perfused lung model with real time data collection and analysis of lung function.
Our primary purpose in making this report has been to describe an isolated perfused lung system which permits the real time collection and analysis of lung mechanical functioning. The distinct advantage of our system lies in its capacity for breath by breath data acquisition and analysis. In addition, because of the modular nature of the components, the system can be readily expanded or contracted depending on the type of experiment being conducted. As configured, the lung mechanic parameters of air flow, lung volume, transpulmonary pressure, pulmonary artery pressure, weight, resistance, elastance (inverse of compliance), and positive end expiratory pressure were monitored, recorded, and evaluated simultaneously throughout the experimental period. We present the results of a 3-h study with control lungs illustrating the stability of these measurements throughout the entire period. Also included is a brief discussion of 3-h studies which show a progressive loss of viability in lungs treated with the redox cycler nitrofurantoin.
Bernard
C E
CE
Department of Biopharmaceutical Sciences, School of Pharmacy, University of California, San Francisco 94143-0446, USA.
Dahlby
R
R
Hoener
B
B
D000818:Animals; D003625:Data Collection / Q000295:instrumentation*; D008168:Lung / Q000502:physiology*; D008297:Male; D010477:Perfusion / Q000295:instrumentation*; D051381:Rats; D017207:Rats, Sprague-Dawley; D012815:Signal Processing, Computer-Assisted