title: Solid phase synthesis and immunogenicity of a VP3 peptide from hepatitis A virus.
abstract: The synthesis of a peptide belonging to the VP3 capsid protein of Hepatitis A virus has been accomplished by the continuous flow Fmoc-polyamide solid phase method. The use of methoxytrimethylbenzenesulphonyl (Mtr) and pentamethylchromansulphonyl (Pmc) as arginine side-chain protecting groups in the presence of tryptophan without lateral protection or protected with t-Boc is discussed. The synthetic VP3 peptide has been administered to mice in different forms: (i) free, (ii) coupled to keyhole limpet hemocyanin, (iii) encapsulated in multilamellar (MLV) liposomes, and (iv) incorporated to a tetrameric branched lysine core. The immune response induced by these preparation is reported.
authors.affiliation: Department of Peptides, CID, CSIC, Barcelona, Spain.
authors.forename: J A
authors.initials: JA
authors.lastname: Pérez
authors.forename: J F
authors.initials: JF
authors.lastname: González-Dankaart
authors.forename: F
authors.initials: F
authors.lastname: Reig
authors.forename: R M
authors.initials: RM
authors.lastname: Pintó
authors.forename: A
authors.initials: A
authors.lastname: Bosch
authors.forename: I
authors.initials: I
authors.lastname: Haro
mesh_terms: D000818:Animals; D002213:Capsid / Q000138:chemical synthesis / Q000276:immunology*; D036022:Capsid Proteins; D004339:Drug Compounding; D005260:Female; D006433:Hemocyanins / Q000276:immunology; D006508:Hepatitis Antibodies / Q000096:biosynthesis; D006507:Hepatovirus / Q000276:immunology*; D008081:Liposomes; D051379:Mice; D010446:Peptide Fragments / Q000138:chemical synthesis / Q000276:immunology*; D014611:Vaccination
chemical_list: D036022:Capsid Proteins; D006508:Hepatitis Antibodies; D008081:Liposomes; D010446:Peptide Fragments; C109000:VP3 peptide (110-121), hepatitis A virus; D006433:Hemocyanins; C032808:keyhole-limpet hemocyanin