Isolation and characterization of mitochondrial DNA-less lines from various mammalian cell lines by application of an anticancer drug, ditercalinium.
Since ethidium bromide was not effective in mouse cell lines for isolating mitochondrial DNA (mtDNA)-less cells (rho zero cells), we examined whether an anticancer drug, ditercalinium (DC), which has been shown to exclude mtDNA from mouse cell lines, could be effective in various mouse and human cell lines. We found that after DC treatment rho zero cells could be isolated from all cell lines of mouse or human origin tested. Moreover, these rho zero cells maintained ability to receive exogenously imported mtDNA and allow its replication and gene expression. These observations suggest that DC eliminates mtDNA from mouse and human cells without affecting the property to receive exogenous mtDNA. Therefore, DC could be applicable to cell lines expressing various differentiated phenotypes for studying whether mtDNA plays a significant role in expression of phenotypes by manipulating mtDNA elimination and reintroduction.
Inoue
K
K
Institute of Biological Sciences, University of Tsukuba, Ibaraki, Japan.
Takai
D
D
Hosaka
H
H
Ito
S
S
Shitara
H
H
Isobe
K
K
LePecq
J B
JB
Segal-Bendirdjian
E
E
Hayashi
J
J
D016475:3T3 Cells; D000818:Animals; D000970:Antineoplastic Agents / Q000494:pharmacology*; D002227:Carbazoles / Q000494:pharmacology*; D002469:Cell Separation; D004272:DNA, Mitochondrial / Q000187:drug effects*; D004996:Ethidium / Q000494:pharmacology; D006801:Humans; D051379:Mice; D014407:Tumor Cells, Cultured
D000970:Antineoplastic Agents; D002227:Carbazoles; D004272:DNA, Mitochondrial; D004996:Ethidium; C042804:ditercalinium