Role of gastrin/CCK-B receptor in the regulation of gastric acid secretion in rat.
The aim of this study was to investigate whether gastrin regulates morphological changes and alpha-subunit gene expression in parietal cells through the gastrin/CCK-B receptor on enterochromaffin-like cells by histamine release. Treatment with 100 mg/kg of YM022, a potent and selective gastrin/CCK-B receptor antagonist, for one week in rats did not alter mRNA levels of histidine decarboxylase or H+, K+-ATPase. However, parietal cell morphology predominantly changed to the resting form, although the serum gastrin concentration was significantly increased. Additional treatment with YM022 and oral omeprazole, 100 mg/kg, for one week markedly suppressed the increases of mRNA levels of histidine decarboxylase and H+, K+-ATPase and completely blocked the morphological transformation of the parietal cells to a stimulated form induced by treatment with omeprazole alone. This indicates that the morphological transformation of parietal cells to an activated form with a subsequent increase in H+, K+-ATPase synthesis caused by hypergastrinemia is mediated by increased histidine decarboxylase gene expression in enterochromaffin-like cells via gastrin/CCK-B receptors.
Tari
A
A
Department of Internal Medicine, Hiroshima Red Cross & Atomic-bomb Survivors Hospital, Japan.
Kamiyasu
T
T
Yonei
Y
Y
Hamada
M
M
Sumii
M
M
Sumii
K
K
Kajiyama
G
G
Dimaline
R
R
D000199:Actins / Q000096:biosynthesis; D000818:Animals; D001569:Benzodiazepines / Q000494:pharmacology; D004791:Enzyme Inhibitors / Q000494:pharmacology; D005744:Gastric Acid / Q000378:metabolism*; D005755:Gastrins / Q000097:blood / Q000502:physiology*; D015870:Gene Expression; D017506:H(+)-K(+)-Exchanging ATPase / Q000096:biosynthesis; D006636:Histamine Release; D006640:Histidine Decarboxylase / Q000037:antagonists & inhibitors / Q000096:biosynthesis; D006727:Hormone Antagonists / Q000494:pharmacology; D008297:Male; D009853:Omeprazole / Q000494:pharmacology; D010295:Parietal Cells, Gastric / Q000187:drug effects*; D054328:Proton Pump Inhibitors; D012333:RNA, Messenger / Q000235:genetics; D051381:Rats; D017208:Rats, Wistar; D011949:Receptors, Cholecystokinin / Q000037:antagonists & inhibitors / Q000502:physiology*
D000199:Actins; D004791:Enzyme Inhibitors; D005755:Gastrins; D006727:Hormone Antagonists; D054328:Proton Pump Inhibitors; D012333:RNA, Messenger; D011949:Receptors, Cholecystokinin; D001569:Benzodiazepines; C087260:YM 022; D017506:H(+)-K(+)-Exchanging ATPase; D006640:Histidine Decarboxylase; D009853:Omeprazole