Purification and characterization of a novel NADP-dependent branched-chain alcohol dehydrogenase from Saccharomyces cerevisiae.
An NADP-dependent branched-chain alcohol dehydrogenase was purified from Saccharomyces cerevisiae var. uvarum grown under anaerobic conditions. Its quaternary structure is monomeric, and it has a molecular mass of 37 kDa and a pI of 5.9. A possible role of the enzyme in flavor production during alcoholic fermentation is discussed.
van Iersel
M F
MF
Department of Food Science and Nutrition, Wageningen Agricultural University, The Netherlands.
Eppink
M H
MH
van Berkel
W J
WJ
Rombouts
F M
FM
Abee
T
T
D000429:Alcohol Oxidoreductases / Q000737:chemistry / Q000302:isolation & purification* / Q000378:metabolism; D000434:Alcoholic Beverages; D000693:Anaerobiosis; D005285:Fermentation; D005524:Food Technology; D006863:Hydrogen-Ion Concentration; D007526:Isoelectric Point; D008970:Molecular Weight; D009249:NADP / Q000378:metabolism; D009994:Osmolar Concentration; D012441:Saccharomyces cerevisiae / Q000201:enzymology* / Q000254:growth & development; D013379:Substrate Specificity
D009249:NADP; D000429:Alcohol Oxidoreductases; C084155:alcohol dehydrogenase (NAD(P)+)