Characterization of the gntT gene encoding a high-affinity gluconate permease in Escherichia coli.
We characterized the gntT gene encoding a high-affinity gluconate permease of Escherichia coli K-12. Primer extension and lacZ-operon fusion analyses revealed that gntT has one strong and two weak promoters, all of which are regulated positively by cAMP-CRP and negatively by GntR. The weak promoters became constitutive when separated from the upstream region including the strong promoter that overlaps a putative GntR-binding sequence. Gluconate-specific uptake activity was observed with cells harboring the gntT plasmid clone, which was enhanced by the presence of gntK encoding gluconate kinase.
Izu
H
H
Department of Biological Chemistry, Faculty of Agriculture, Yamaguchi University, Japan.
Kawai
T
T
Yamada
Y
Y
Aoshima
H
H
Adachi
O
O
Yamada
M
M
D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000235:genetics* / Q000378:metabolism*; D001483:Base Sequence; D001692:Biological Transport; D002241:Carbohydrates; D002352:Carrier Proteins; D000242:Cyclic AMP / Q000494:pharmacology; D002373:Cyclic AMP Receptor Protein / Q000378:metabolism; D004926:Escherichia coli / Q000201:enzymology / Q000235:genetics*; D029968:Escherichia coli Proteins; D015964:Gene Expression Regulation, Bacterial / Q000187:drug effects / Q000502:physiology*; D005798:Genes, Bacterial / Q000502:physiology; D005942:Gluconates / Q000378:metabolism / Q000494:pharmacology; D007700:Kinetics; D026901:Membrane Transport Proteins / Q000235:genetics*; D008969:Molecular Sequence Data; D009875:Operator Regions, Genetic / Q000235:genetics; D017853:Phosphotransferases (Alcohol Group Acceptor) / Q000502:physiology; D011401:Promoter Regions, Genetic / Q000235:genetics; D017422:Sequence Analysis, DNA
D001426:Bacterial Proteins; D002241:Carbohydrates; D002352:Carrier Proteins; D002373:Cyclic AMP Receptor Protein; D029968:Escherichia coli Proteins; D005942:Gluconates; D026901:Membrane Transport Proteins; C104633:gluconate permease, E coli; D000242:Cyclic AMP; D017853:Phosphotransferases (Alcohol Group Acceptor); C020578:gluconokinase; C030691:gluconic acid