The protective effect of zinc on rosin and resin acid toxicity in human polymorphonuclear leukocytes and human gingival fibroblasts in vitro.
Combinations of rosin and zinc are used in dentistry as components of periodontal dressings and cements and as root canal sealers. The composition and properties of rosins differ largely depending on source and refinement processes. Rosin (colophony) is composed of approximately 70% resin acids. In order to study the toxic effects of different natural rosins and purified resin acids and the detoxifying effects of zinc, these compounds were analyzed and tested on human polymorphonuclear leukocytes (PMN cells) and human gingival fibroblasts using the radiochromium release method. The rosins and the pure resin acids showed a strong dose-related cytotoxicity, which was inhibited by increased zinc concentrations. The purified resin acids (isopimaric, levopimaric, and neoabietic acid) were more toxic than the natural rosins. The contents of these resin acids might explain the difference in toxicity of the rosins tested. It is concluded that rosin and zinc are not to be considered inert compounds and that the cytoprotective effects of zinc and its role in dentistry products merit further investigations.
Sunzel
B
B
Department of Oral and Maxillofacial Surgery, University of Umeå, Sweden.
Söderberg
T A
TA
Johansson
A
A
Hallmans
G
G
Gref
R
R
D000328:Adult; D016923:Cell Death / Q000187:drug effects; D002849:Chromatography, Gas; D003738:Dental Cements / Q000737:chemistry / Q000633:toxicity; D003764:Dental Materials / Q000737:chemistry / Q000633:toxicity*; D005347:Fibroblasts / Q000187:drug effects; D005881:Gingiva / Q000166:cytology / Q000187:drug effects*; D006801:Humans; D066298:In Vitro Techniques; D008422:Materials Testing; D009504:Neutrophils / Q000187:drug effects*; D010511:Periodontal Dressings; D010936:Plant Extracts / Q000737:chemistry / Q000633:toxicity; D012116:Resins, Plant / Q000737:chemistry / Q000633:toxicity*; D012387:Root Canal Filling Materials / Q000737:chemistry / Q000633:toxicity; D015032:Zinc / Q000494:pharmacology*
D003738:Dental Cements; D003764:Dental Materials; D010936:Plant Extracts; D012116:Resins, Plant; D012387:Root Canal Filling Materials; C018620:oleoresins; C013893:rosin; D015032:Zinc