[Reproducibility of aDNA typing].
The reproducibility of short tandem repeat (STR) amplification of aDNA extracts is limited by means of formation of artifacts during PCR. One of these artifacts, the so called allelic-dropout (failure of the amplification of alleles), may result in false-homozygote typing of a sample, if genotyping is based on the analysis of a single amplification product. 30 tooth- and bone samples collected from 17 individuals of three burial sites were investigated in a blind test. Using the polymerase chain reaction (PCR) the two independent segregating STR-loci HUMVWA and HUMTH01 were amplified. Corresponding to a mathematical estimation, multiple amplifications of each sample and genelocus were carried out. The results of this genotyping were compared intraindividually.
Schmerer
W M
WM
Institut für Anthropologie, Universität Göttingen.
Hummel
S
S
Herrmann
B
B
D001842:Bone and Bones / Q000378:metabolism*; D004247:DNA / Q000235:genetics*; D005784:Gene Amplification; D005838:Genotype; D006720:Homozygote; D006801:Humans; D010164:Paleopathology; D012091:Repetitive Sequences, Nucleic Acid / Q000235:genetics*; D015203:Reproducibility of Results; D014070:Tooth / Q000378:metabolism*
D004247:DNA