Effect of dialyzer membranes on beta-2 microglobulin production in Thai hemodialysis patients.
Responses to different types of dialyzer membranes in an Asian population may differ from those of a Caucasian population. Comparative studies on the effects of different dialyzer membranes on beta-2 microglobulin production are also limited. Therefore, we conducted this study to determine the effects of different dialyzer membranes on in vitro mononuclear cell production of beta-2 microglobulin in 9 Thai hemodialysis patients. Each patient was dialysed with 4 different types of dialyzer, including cuprophane (CUP), cellulose diacetate (CD), polysulphone (PS), and polyacrylonitrile membrane (PAN), each for a 1-month period in a randomized sequence. Mononuclear cell culture was done by taking an immediate post-dialysis blood sample at the end of the 1-month period. Beta-2 microglobulin production from cell culture was determined 24 hours later. Mononuclear cell culture and determination of beta-2 microglobulin production from the culture were also done in 10 normal controls and 10 predialysis ESRD patients. The beta-2 microglobulin productions (microgram/L) were shown as follows; Control CUP CD PS PAN [table: see text] (*p < 0.05 compared to cuprophane membrane).


CONCLUSIONS
polysulphone and polyacrylonitrile membrane induced significantly less beta-2 microglobulin production compared to cuprophane and slightly less compared to cellulose diacetate membrane.
Domrongkitchaiporn
S
S
Department of Medicine, Ramathibodi Hospital, Mahidol University, Bangkok, Thailand.
Chuncharunee
S
S
Archararit
N
N
Atamasirikul
K
K
Vanichakarn
S
S
D000180:Acrylic Resins; D000328:Adult; D000704:Analysis of Variance; D001672:Biocompatible Materials; D018929:Cell Culture Techniques; D002482:Cellulose / Q000031:analogs & derivatives; D005260:Female; D006801:Humans; D008297:Male; D008567:Membranes, Artificial; D008875:Middle Aged; D011108:Polymers; D006435:Renal Dialysis / Q000295:instrumentation*; D013450:Sulfones; D013785:Thailand; D001613:beta 2-Microglobulin / Q000096:biosynthesis* / Q000378:metabolism
D000180:Acrylic Resins; D001672:Biocompatible Materials; D008567:Membranes, Artificial; D011108:Polymers; D013450:Sulfones; D001613:beta 2-Microglobulin; C010504:polyacrylonitrile; C017662:polysulfone P 1700; C005062:acetylcellulose; D002482:Cellulose; C009865:cuprammonium cellulose