title: Cell attachment to, and contraction of, the retina in vitro.
abstract: OBJECTIVE
  To examine the behavior of fibroblasts and retinal pigment epithelial cells after attachment to the retinal surface in vitro to elucidate the pathobiology of the early stages of epiretinal membrane formation.
  
  
  METHODS
  Human retinal pigment epithelial (HRPE) cells and bovine Tenon's capsule fibroblasts (BTFs) were seeded onto the surface of bovine retinal explants maintained in organ culture. The changes induced in the underlying retina, including contraction, were assessed during a period of up to 10 days. Immunohistochemistry was used to assess proliferation of the seeded cells and to determine deposition of extracellular matrix.
  
  
  RESULTS
  Explants of bovine neuroretina were maintained in organ culture, with good morphologic preservation of the inner limiting lamina and inner retinal layers, for 7 to 10 days. The HRPE cells and the BTFs attached to the retinal surface and exerted tractional forces, producing partial- and full-thickness retinal folding. Contraction commenced within 24 hours of attachment of the cells and continued for several days, with most of the contraction occurring within the next 48 to 72 hours. The HRPE cells and BTFs were found to be equally contractile. Deposition of cellular fibronectin (but not collagen type I) was demonstrated.
  
  
  CONCLUSIONS
  The contractile cellular membranes generated in this organ culture system exhibit many of the morphologic and functional features of epiretinal membranes found in the early stages of proliferative vitreoretinopathy.
authors.affiliation: Department of Ophthalmology, Manchester Royal Eye Hospital, United Kingdom.
authors.forename: D
authors.initials: D
authors.lastname: Allamby
authors.forename: D
authors.initials: D
authors.lastname: Foreman
authors.forename: L
authors.initials: L
authors.lastname: Carrington
authors.forename: D
authors.initials: D
authors.lastname: McLeod
authors.forename: M
authors.initials: M
authors.lastname: Boulton
mesh_terms: D000328:Adult; D000368:Aged; D000818:Animals; D002417:Cattle; D002448:Cell Adhesion / Q000502:physiology*; D002455:Cell Division; D003239:Connective Tissue Cells; D016326:Extracellular Matrix Proteins / Q000378:metabolism; D005347:Fibroblasts / Q000166:cytology / Q000378:metabolism / Q000502:physiology*; D006801:Humans; D007124:Immunoenzyme Techniques; D008855:Microscopy, Electron, Scanning; D008875:Middle Aged; D009924:Organ Culture Techniques; D010857:Pigment Epithelium of Eye / Q000166:cytology / Q000378:metabolism / Q000502:physiology*; D012160:Retina / Q000378:metabolism / Q000502:physiology*; D018630:Vitreoretinopathy, Proliferative / Q000503:physiopathology
chemical_list: D016326:Extracellular Matrix Proteins