title: Genomic structure and embryonic expression of the rat type 1 vasoactive intestinal polypeptide receptor gene.
abstract: The genomic structure of the rat vasoactive intestinal polypeptide type 1 receptor (VIPR I) gene was characterized using polymerase chain reactions (PCR) and DNA sequencing. These studies show that the rat VIPR I gene spans more than 20 kb of DNA sequence and includes thirteen exons separated by twelve introns, ranging from 0.2 to 4.5 kb. VIP is known to play an important role early in embryonic development. To elucidate the receptor-mediated biological functions of VIP in development, the temporal and spatial expression of VIPR I during rat embryonic development was examined in this study. Using in situ hybridization histochemistry with 35S-labeled riboprobe, VIPR I mRNA was detected as early as embryonic day 11 (E11), in the neuroepithelium, foregut, heart and in cells lining the blood vessels. From E14 through E18, VIPR I mRNA was detected in multiple tissues derived from all three germ layers. These include neuronal cells in brain and spinal cord (ectoderm); smooth muscle cells of intestines, cells lining the wall of the heart and blood vessels, kidney, adrenal gland (mesoderm); epithelial cells of airway and of liver (endoderm). The early onset and wide tissue expression of VIPR I suggests that binding of VIP to this receptor may play an important role in rat embryonic development.
authors.affiliation: Division of Endocrinology and Metabolism, Cedars-Sinai Research Institute-UCLA School of Medicine, Los Angeles, CA 90048, USA.
authors.forename: L
authors.initials: L
authors.lastname: Pei
mesh_terms: D000818:Animals; D004622:Embryo, Mammalian / Q000502:physiology; D018507:Gene Expression Regulation, Developmental; D017403:In Situ Hybridization; D012333:RNA, Messenger; D051381:Rats; D018005:Receptors, Vasoactive Intestinal Peptide / Q000235:genetics*; D051238:Receptors, Vasoactive Intestinal Polypeptide, Type I; D014018:Tissue Distribution
chemical_list: D012333:RNA, Messenger; D018005:Receptors, Vasoactive Intestinal Peptide; D051238:Receptors, Vasoactive Intestinal Polypeptide, Type I