Effect of 4-methylumbelliferone on cell-free synthesis of hyaluronic acid.
Human skin fibroblasts were cultured in the presence of 0.5 mM 4-methylumbelliferone for 12 h, and cell-free synthesis of hyaluronic acid was performed using membrane-rich fraction from the cells. The preincubation of the cells with 4-methylumbelliferone reduced hyaluronic acid synthesis to 15% of that of non-preincubated cells, although its chain length was not changed. On the other hand, without preincubation of the cells with 4-methylumbelliferone, hyaluronic acid synthesis was not changed even when 4-methylumbelliferone was added directly to the reaction mixture. These results suggest that 4-methylumbelliferone represses the expression of hyaluronic acid synthase on the cell surface.
Nakamura
T
T
Department of Biochemistry, Hirosaki University School of Medicine, Japan.
Funahashi
M
M
Takagaki
K
K
Munakata
H
H
Tanaka
K
K
Saito
Y
Y
Endo
M
M
D002462:Cell Membrane / Q000378:metabolism; D002474:Cell-Free System; D002478:Cells, Cultured; D004791:Enzyme Inhibitors / Q000494:pharmacology; D005347:Fibroblasts / Q000378:metabolism; D014453:Glucuronosyltransferase / Q000037:antagonists & inhibitors; D016695:Glycosyltransferases; D006801:Humans; D000076002:Hyaluronan Synthases; D006820:Hyaluronic Acid / Q000037:antagonists & inhibitors* / Q000096:biosynthesis*; D006923:Hymecromone / Q000494:pharmacology*; D008565:Membrane Proteins; D014166:Transferases; D029867:Xenopus Proteins
D004791:Enzyme Inhibitors; D008565:Membrane Proteins; D029867:Xenopus Proteins; D006923:Hymecromone; D006820:Hyaluronic Acid; D014166:Transferases; D016695:Glycosyltransferases; D014453:Glucuronosyltransferase; C056944:HAS1 protein, Xenopus; D000076002:Hyaluronan Synthases