Orientation of photosynthetic reaction center reconstituted in neutral and charged liposomes.
The photosynthetic reaction center from the photosynthetic bacterium Rhodobacter sphaeroides was reconstituted into neutral, positively charged, or negatively charged liposomes. About 70% of photosynthetic reaction centers were reconstituted in the proteoliposomes exposing their H-subunit outside with positively charged lipids while only 30-40% of them were in the same topological orientation with neutral or negatively charged lipids.
Hara
M
M
National Institute of Bioscience and Human-Technology, AIST, MITI, Ibaraki, Japan.
Ueno
T
T
Fujii
T
T
Yang
Q
Q
Asada
Y
Y
Miyake
J
J
D003574:Cytochrome c Group / Q000378:metabolism; D004579:Electron Transport; D050356:Lipid Metabolism; D008055:Lipids / Q000737:chemistry; D008081:Liposomes / Q000737:chemistry*; D008567:Membranes, Artificial; D010316:Particle Size; D010788:Photosynthesis / Q000502:physiology*; D012242:Rhodobacter sphaeroides / Q000737:chemistry / Q000378:metabolism*
D003574:Cytochrome c Group; D008055:Lipids; D008081:Liposomes; D008567:Membranes, Artificial