Purification of recombinant human precursor acid alpha-glucosidase.
Large quantities of recombinant acid alpha-glucosidase are needed for in vivo experimentation of enzyme replacement therapy in Pompe disease. We describe a new purification method for the purification of this recombinant enzyme from tissue culture medium consisting of concanavalin A affinity chromatography, hydrophobic interaction chromatography, affinity chromatography on Superdex, and anion exchange chromatography. The new method is amenable to scale up, and has increased speed, and improved reproducibility with similar high yield and purification efficiency when compared to previous methods.
Van Hove
J L
JL
Department of Pediatrics, Duke University Medical Center, Durham, NC, USA.
Yang
H W
HW
Oliver
L M
LM
Pennybacker
M F
MF
Chen
Y T
YT
D000818:Animals; D016466:CHO Cells / Q000201:enzymology; D002845:Chromatography / Q000379:methods*; D006224:Cricetinae; D005347:Fibroblasts / Q000187:drug effects; D006009:Glycogen Storage Disease Type II / Q000201:enzymology; D006801:Humans; D000520:alpha-Glucosidases / Q000302:isolation & purification* / Q000378:metabolism / Q000494:pharmacology
D000520:alpha-Glucosidases