title: Differential metabolism of dynorphins in substantia nigra, striatum, and hippocampus.
abstract: To map the proteolytic enzymes metabolizing dynorphins in brain structures, size-exclusion chromatography linked to electrospray ionization mass spectrometry was used. Enzymes extracted from rat hippocampus, striatum, and substantia nigra were tested for their capability of converting dynorphin-related peptides. Dynorphin A was the most resistant to proteolytic conversion, whereas Big dynorphin and dynorphin B-29 were slowly converted to dynorphin A and dynorphins A and B, respectively. Dynorphin B and alpha-neoendorphin were the least resistant. Dynorphin B was rapidly converted to Leu-enkephalin in the striatum and hippocampus but to Leu-enkephalin-Arg6 in the substantia nigra. alpha-Neoendorphin was converted to Leu-enkephalin in all tissues investigated.
authors.affiliation: Department of Clinical Neuroscience, Karolinska Institute, Stockholm, Sweden. johasa@cmm.ki.se
authors.forename: J
authors.initials: J
authors.lastname: Sandin
authors.forename: K
authors.initials: K
authors.lastname: Tan-No
authors.forename: L
authors.initials: L
authors.lastname: Kasakov
authors.forename: I
authors.initials: I
authors.lastname: Nylander
authors.forename: A
authors.initials: A
authors.lastname: Winter
authors.forename: J
authors.initials: J
authors.lastname: Silberring
authors.forename: L
authors.initials: L
authors.lastname: Terenius
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D002851:Chromatography, High Pressure Liquid; D003342:Corpus Striatum / Q000378:metabolism*; D004399:Dynorphins / Q000737:chemistry / Q000378:metabolism*; D004723:Endorphins / Q000737:chemistry / Q000378:metabolism; D004743:Enkephalin, Leucine / Q000031:analogs & derivatives / Q000737:chemistry / Q000378:metabolism; D006624:Hippocampus / Q000378:metabolism*; D008297:Male; D013058:Mass Spectrometry; D011498:Protein Precursors / Q000737:chemistry / Q000378:metabolism; D051381:Rats; D013378:Substantia Nigra / Q000378:metabolism*; D014018:Tissue Distribution
chemical_list: D004723:Endorphins; D011498:Protein Precursors; D004743:Enkephalin, Leucine; D004399:Dynorphins; C026768:enkephalin-Leu, Arg(6)-; C036200:rimorphin