Evaluation of a new version of the EnviroAmp Legionella kit for the detection of legionellae in water samples by the polymerase chain reaction.
The detection of Legionella sp. in various types of environmental water samples was evaluated using a revised version of the EnviroAmp Legionella kit (Perkin-Elmer), which is based on the polymerase chain reaction (PCR). The new kit employs a modified protocol for the pretreatment of water samples, which is designed to further reduce the inhibitory action of some samples to the PCR. The results of the new kit were compared with those of culture. Seventy-four water samples were examined. Of these samples, 51 were taken from hospital water systems, 16 were household water samples, 5 were from surface water, and 2 were rain water. These samples were examined both by PCR and by culture on MWY Legionella selective agar. Of the 74 samples, 46 (62%) were positive by both test systems. Twenty-five samples (34%) were positive by PCR, but negative by culture. While 3 samples (4%) were negative by both test methods, no sample was negative by PCR and positive by culture. Statistical analysis revealed high positive predictive values of PCR, when L. pneumophila was detected or when the genus Legionella was detected at high intensity. Negative predictive values were high, when the PCR was negative or only weakly positive for the genus Legionella.
Okpara
J
J
Abt. Hygiene und Med. Mikrobiologie, Hygiene-Institut der Universität Heidelberg, Germany.
Maiwald
M
M
Borneff
M
M
Windeler
J
J
Sonntag
H G
HG
D001431:Bacteriological Techniques; D015169:Colony Count, Microbial; D004269:DNA, Bacterial / Q000302:isolation & purification; D006801:Humans; D007875:Legionella / Q000235:genetics / Q000302:isolation & purification*; D016133:Polymerase Chain Reaction; D011933:Reagent Kits, Diagnostic; D012680:Sensitivity and Specificity; D014871:Water Microbiology
D004269:DNA, Bacterial; D011933:Reagent Kits, Diagnostic