title: The intracellular calcium increase at fertilization in Urechis caupo oocytes: activation without waves.
abstract: The intracellular Ca2+ (Cai) increase at fertilization of the marine worm Urechis caupo (Echiura) was studied with conventional and confocal epifluorescence microscopy in oocytes microinjected with calcium green dextran or dually labeled with the calcium-insensitive dye tetramethylrhodamine dextran. Calcium green fluorescence was also measured with a photomultiplier system while the oocyte membrane potential was recorded and manipulated. The results show that Cai rises simultaneously around the oocyte cortex and peaks slightly later in the nucleoplasm. The Cai rise coincides with the initiation of the fertilization potential and we conclude that it is due primarily to external Ca2+ entering through the voltage-gated Ca2+ action potential channels that open during the fertilization potential because: (1) current clamping the oocyte membrane potential to positive values in the absence of sperm produces a similar Cai increase, (2) external Ca2+ is required, (3) and the confocal images are consistent with this mechanism. External application of sperm acrosomal peptide (P23) also caused a Cai increase that was inhibited in the presence of CoCl2. Cai and pHi (measured with BCECF dextran) were manipulated in experiments employing microinjection of BAPTA (to chelate Cai), external application of NH4Cl (to increase pHi) and CoCl2 (to block Ca2+ channels), and fertilization of eggs in pH 7 seawater (Cai increase without pHi increase). The results showed that increases in both Cai and pHi are required for GVBD; neither alone is sufficient. However, although nuclear and cytoplasmic Ca2+ levels tended to parallel each other in oocytes fertilized at pH 7, and during the initial Cai response in oocytes fertilized at pH 8, there was a disproportionate fluorescence increase in the nucleoplasm of the latter prior to GVBD which could not be explained by any artifact we tested, suggesting there may be a selective increase in nuclear Ca2+ associated with GVBD. Finally, electrophysiological experiments with BAPTA-injected oocytes showed that the opening of the fertilization potential Na+ channels was Ca2+-independent, (although they did not close at the normal time). These and earlier results suggest that Urechis sperm may activate oocytes by interacting directly with the Na+ channels or associated receptors.
authors.affiliation: Facultad de Ciencias, Universidad Autónoma de Baja California, Ensenada, B.C., 22800, México.
authors.forename: J L
authors.initials: JL
authors.lastname: Stephano
authors.forename: M C
authors.initials: MC
authors.lastname: Gould
mesh_terms: D000643:Ammonium Chloride / Q000494:pharmacology; D000818:Animals; D000845:Annelida / Q000502:physiology*; D002118:Calcium / Q000378:metabolism*; D003035:Cobalt / Q000494:pharmacology; D005260:Female; D005306:Fertilization; D005456:Fluorescent Dyes; D007700:Kinetics; D008297:Male; D018613:Microscopy, Confocal; D008856:Microscopy, Fluorescence; D009865:Oocytes / Q000166:cytology / Q000187:drug effects / Q000502:physiology*; D013094:Spermatozoa / Q000502:physiology; D013997:Time Factors
chemical_list: D005456:Fluorescent Dyes; D000643:Ammonium Chloride; D003035:Cobalt; C018021:cobaltous chloride; D002118:Calcium