title: Infection of a human retinal pigment epithelial cell line with human herpesvirus 6 variant A.
abstract: A retinal pigment epithelial (RPE) cell line (K-1034) was examined for its susceptibility to human herpesvirus 6 variant A (HHV-6A). Exposure of K-1034 cells to HHV-6A induced the formation of multinucleated giant cells, which was suppressed by an inhibitor of viral DNA synthesis. In the giant cells, herpesvirus nucleocapsids were demonstrated by electron microscopy and the viral glycoprotein B was detected by immunofluorescence assay. These results indicate that K-1034 cells are susceptible to HHV-6A and suggest that HHV-6A has an ability to directly destroy epithelial cells.
authors.affiliation: Department of Pathology, National Institute of Health, Tokyo, Japan.
authors.forename: Y
authors.initials: Y
authors.lastname: Arao
authors.forename: S
authors.initials: S
authors.lastname: Soushi
authors.forename: Y
authors.initials: Y
authors.lastname: Sato
authors.forename: E
authors.initials: E
authors.lastname: Moriishi
authors.forename: Y
authors.initials: Y
authors.lastname: Ando
authors.forename: M
authors.initials: M
authors.lastname: Yamada
authors.forename: J
authors.initials: J
authors.lastname: Padilla
authors.forename: F
authors.initials: F
authors.lastname: Uno
authors.forename: S
authors.initials: S
authors.lastname: Nii
authors.forename: T
authors.initials: T
authors.lastname: Kurata
mesh_terms: D002478:Cells, Cultured; D018920:Coculture Techniques; D003588:Cytopathogenic Effect, Viral; D004279:DNA, Viral / Q000096:biosynthesis; D004847:Epithelial Cells / Q000821:virology*; D015774:Ganciclovir / Q000494:pharmacology; D015726:Giant Cells; D015654:Herpesvirus 6, Human / Q000472:pathogenicity* / Q000502:physiology; D006801:Humans; D019384:Nucleic Acid Synthesis Inhibitors / Q000494:pharmacology; D010746:Phosphonoacetic Acid / Q000494:pharmacology; D010857:Pigment Epithelium of Eye / Q000821:virology*; D014759:Viral Envelope Proteins / Q000032:analysis
chemical_list: D004279:DNA, Viral; D019384:Nucleic Acid Synthesis Inhibitors; D014759:Viral Envelope Proteins; C077311:glycoprotein B, human herpesvirus-6; D010746:Phosphonoacetic Acid; D015774:Ganciclovir