title: Expression of peripherin, NADPH-diaphorase and NOS in the adult rat neocortex.
abstract: Peripherin is mainly expressed in peripheral neurones and in CNS neurones which extend axons into peripheral nerves. However, this intermediate filament protein has also been detected in a few other neurones entirely located within the CNS. The present study focuses on the adult rat neocortex. Peripherin immunoreactive (P+) neuronal somata and their neuritic extensions were observed in cortical layers II, III, V and VI, while a few P+ nerve fibres could be seen in layer I. All the P+ neurones could be selectively stained using reduced nicotinamide adenine dinucleotide phosphate diaphorase (NADPH-d) histochemistry, a typical feature of aspiny neurones. Some of the P+ neurones could also be immunostained with an antibody raised against nitric oxide synthase (NOS). These results provide evidence that peripherin is present in a discrete population of aspiny interneurones of the adult rat neocortex. The functional significance of the co-expression of peripherin and NOS needs further investigation.
authors.affiliation: Neurobiologie, URA CNRS 1448, Université René Descartes, Paris, France.
authors.forename: F
authors.initials: F
authors.lastname: Rhrich-Haddout
authors.forename: P
authors.initials: P
authors.lastname: Klosen
authors.forename: M M
authors.initials: MM
authors.lastname: Portier
authors.forename: J C
authors.initials: JC
authors.lastname: Horvat
mesh_terms: D000818:Animals; D015153:Blotting, Western; D005260:Female; D007150:Immunohistochemistry; D007381:Intermediate Filament Proteins / Q000096:biosynthesis*; D007395:Interneurons / Q000201:enzymology*; D008562:Membrane Glycoproteins / Q000096:biosynthesis*; D009252:NADPH Dehydrogenase / Q000096:biosynthesis*; D019579:Neocortex / Q000166:cytology / Q000201:enzymology / Q000378:metabolism*; D009419:Nerve Tissue Proteins / Q000096:biosynthesis*; D019001:Nitric Oxide Synthase / Q000096:biosynthesis*; D064531:Peripherins; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D007381:Intermediate Filament Proteins; D008562:Membrane Glycoproteins; D009419:Nerve Tissue Proteins; D064531:Peripherins; D019001:Nitric Oxide Synthase; D009252:NADPH Dehydrogenase