title: Sequence analysis demonstrates that VP6, NSP1 and NSP4 genes of Indian neonatal rotavirus strain 116E are of human origin.
abstract: We have sequenced the genes encoding the inner capsid protein VP6 and the nonstructural proteins NSP1 and NSP4 of the Indian neonatal serotype P8[11]G9 human/bovine reassortant candidate vaccine rotavirus strain 116E. These three genes share a high degree of sequence and deduced amino acid homology with human prototype strain Wa. Our results confirm and extend those of previous RNA-RNA hybridization studies which suggested that these genes are of human origin, and will facilitate examination of the host immune response to 116E induced by natural infection and vaccination.
authors.affiliation: Division of Viral and Rickettsial Diseases, National Center for Infectious Diseases, Centers for Disease Control and Prevention, Department of Health and Human Services, Atlanta, Georgia, USA.
authors.forename: N A
authors.initials: NA
authors.lastname: Cunliffe
authors.forename: B K
authors.initials: BK
authors.lastname: Das
authors.forename: M
authors.initials: M
authors.lastname: Ramachandran
authors.forename: M K
authors.initials: MK
authors.lastname: Bhan
authors.forename: R I
authors.initials: RI
authors.lastname: Glass
authors.forename: J R
authors.initials: JR
authors.lastname: Gentsch
mesh_terms: D000595:Amino Acid Sequence; D000956:Antigens, Viral; D002213:Capsid / Q000235:genetics*; D036022:Capsid Proteins; D017124:Conserved Sequence; D005814:Genes, Viral; D006023:Glycoproteins / Q000235:genetics*; D006801:Humans; D007194:India; D007231:Infant, Newborn; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D012401:Rotavirus / Q000737:chemistry / Q000145:classification / Q000235:genetics*; D017421:Sequence Analysis; D017386:Sequence Homology, Amino Acid; D014118:Toxins, Biological; D017361:Viral Nonstructural Proteins / Q000235:genetics*; D015678:Viral Structural Proteins / Q000235:genetics*
chemical_list: D000956:Antigens, Viral; D036022:Capsid Proteins; D006023:Glycoproteins; C081369:NS28 protein, rotavirus; D014118:Toxins, Biological; C061332:VP6 protein, Rotavirus; D017361:Viral Nonstructural Proteins; D015678:Viral Structural Proteins; C104282:nsp1 protein, Rotavirus