Computerized image analysis of sister chromatid exchanges in Chinese hamster ovary cells.
In this paper we show that sister chromatid exchanges (SCE) can be analysed by computer-supported determination of areas and shapes of darkly stained chromatid sections. In combination with appropriate statistical analyses, our method leads to measurements of SCE frequencies in neocarzinostatin-treated Chinese hamster ovary cells which are nearly as precise as the ones obtained by classical microscopical analyses. We discuss our data as an approach to the development of a fully automated SCE scoring system.
Schunck
C
C
Department of Genetics, University of Essen, Germany.
Obe
G
G
D000818:Animals; D016466:CHO Cells; D006224:Cricetinae; D004249:DNA Damage; D007091:Image Processing, Computer-Assisted / Q000379:methods*; D012854:Sister Chromatid Exchange / Q000187:drug effects; D009353:Zinostatin / Q000494:pharmacology
D009353:Zinostatin