Evidence that peptides derived from the disintegrin domain of primate fertilin and containing the ECD motif block the binding of human spermatozoa to the zona-free hamster oocyte.
The binding of human spermatozoa to, and penetration of, zona-free hamster oocytes were significantly inhibited in the presence of (i) a 28-residue peptide corresponding to part of the disintegrin-like domain of monkey fertilin beta, or (ii) a hexapeptide containing the ECD motif found in the disintegrin-like domain of monkey and human fertilin beta, or (iii) a hexapeptide containing an RGD motif, implicated in integrin recognition. Polyclonal antibodies raised against the 28-residue peptide were used in conjunction with immunobeads to demonstrate that this peptide bound to the oolemma of zona-free hamster oocytes. These data support the view that the ECD motif is involved in the recognition of the oolemma receptor by human fertilin.
Gichuhi
P M
PM
University Department of Obstetrics and Gynaecology, St. Michael's Hospital, Bristol, UK.
Ford
W C
WC
Hall
L
L
D051722:ADAM Proteins; D000595:Amino Acid Sequence; D000818:Animals; D006224:Cricetinae; D019483:Disintegrins / Q000737:chemistry / Q000494:pharmacology*; D005260:Female; D000072196:Fertilins; D006801:Humans; D008252:Macaca fascicularis; D008297:Male; D008562:Membrane Glycoproteins / Q000737:chemistry / Q000494:pharmacology*; D008666:Metalloendopeptidases / Q000737:chemistry / Q000494:pharmacology*; D008969:Molecular Sequence Data; D009842:Oligopeptides / Q000737:chemistry / Q000494:pharmacology; D009865:Oocytes / Q000187:drug effects / Q000502:physiology*; D010446:Peptide Fragments / Q000737:chemistry / Q000494:pharmacology; D010455:Peptides / Q000737:chemistry / Q000494:pharmacology; D013084:Sperm-Ovum Interactions / Q000187:drug effects*; D013094:Spermatozoa / Q000187:drug effects / Q000502:physiology*; D015044:Zona Pellucida / Q000502:physiology
D019483:Disintegrins; D008562:Membrane Glycoproteins; D009842:Oligopeptides; D010446:Peptide Fragments; D010455:Peptides; C047981:arginyl-glycyl-aspartic acid; D051722:ADAM Proteins; C000606454:ADAM2 protein, human; D000072196:Fertilins; D008666:Metalloendopeptidases