Determination of disodium cromoglycate in human urine by high-performance liquid chromatography with post-column photoirradiation-fluorescence detection.
For the determination of disodium cromoglycate in urine, a fluorimetric method using HPLC post-column photoirradiation has been developed. The mobile phase consisted of a 35 mmol l-1 phosphate buffer (pH 8)-methanol (7 + 3, %v/v) containing 75 mmol l-1 hydrogen peroxide and 20 mmol l-1 18-crown-6. The 18-crown-6 was used for separation adjustment of the disodium cromoglycate in the urine sample. Photoirradiation was carried out in tubing wound around a germicidal light in a reactor equipped with an air-cooling fan. The fluorescence was monitored with excitation at 325 nm and emission at 448 nm. The calibration graph for disodium cromoglycate was linear over the range 38-2340 ng ml-1 using an injection volume of 100 microliters. The pretreatment of the urine samples consisted of diluting and filtering steps. The mean recovery of disodium cromoglycate from urine was 99.1 +/- 2.4% (n = 6).
Mawatari
K
K
Department of Analytical Chemistry, Faculty of Pharmacutical Sciences, Teikyo University, Kanagawa, Japan.
Mashiko
S
S
Sate
Y
Y
Usui
Y
Y
Iinuma
F
F
Watanabe
M
M
D000280:Administration, Inhalation; D018927:Anti-Asthmatic Agents / Q000008:administration & dosage / Q000652:urine*; D002851:Chromatography, High Pressure Liquid; D004205:Cromolyn Sodium / Q000008:administration & dosage / Q000652:urine*; D005470:Fluorometry; D006801:Humans; D008297:Male
D018927:Anti-Asthmatic Agents; D004205:Cromolyn Sodium