title: Antibodies against dengue virus E protein peptide bind to human plasminogen and inhibit plasmin activity.
abstract: Both mice and rabbits immunized with dengue virus E protein peptide spanning amino acids 100-119 (D4E) produced antibodies that reacted not only with the D4E peptide itself but also with human plasminogen, as shown by ELISA and Western blot. Sera from dengue virus-hyperimmunized mice and dengue patients also contained antibodies against D4E and plasminogen. Furthermore, such sera all contained plasmin inhibitory activity. Using affinity-purified anti-D4E antibodies and free D4E peptide for competitive inhibition, we demonstrated that the inhibition of plasmin activity was due to anti-D4E antibodies rather than other substances in the sera. Taken together, these results suggest dengue virus E protein amino acids 100-119 are a cross-reactive immunogenic region, and antibodies against this region may interfere with human fibrinolysis.
authors.affiliation: Department of Microbiology and Immunology, College of Medicine, National Cheng Kung University, Tainan, Taiwan, ROC.
authors.forename: Y H
authors.initials: YH
authors.lastname: Huang
authors.forename: B I
authors.initials: BI
authors.lastname: Chang
authors.forename: H Y
authors.initials: HY
authors.lastname: Lei
authors.forename: H S
authors.initials: HS
authors.lastname: Liu
authors.forename: C C
authors.initials: CC
authors.lastname: Liu
authors.forename: H L
authors.initials: HL
authors.lastname: Wu
authors.forename: T M
authors.initials: TM
authors.lastname: Yeh
mesh_terms: D000818:Animals; D000914:Antibodies, Viral / Q000276:immunology*; D000956:Antigens, Viral / Q000276:immunology; D003716:Dengue Virus / Q000276:immunology*; D005260:Female; D005341:Fibrinolysin / Q000037:antagonists & inhibitors* / Q000276:immunology; D006801:Humans; D051379:Mice; D008807:Mice, Inbred BALB C; D010455:Peptides / Q000276:immunology; D010958:Plasminogen / Q000276:immunology*; D011817:Rabbits; D014759:Viral Envelope Proteins / Q000276:immunology*
chemical_list: D000914:Antibodies, Viral; D000956:Antigens, Viral; C051821:E-glycoprotein, Dengue virus type 2; D010455:Peptides; D014759:Viral Envelope Proteins; D010958:Plasminogen; D005341:Fibrinolysin