title: Natural spillover of a distinctly Canidae-associated biotype of rabies virus into an expanded wildlife host range in southern Africa.
abstract: Rabies enzootics in southern Africa are associated with two genetically distinct groups of viruses, thought to be adapted to two different sets of host species. The virus groups are referred to as the canid biotype (infecting carnivores of the family Canidae) and the viverrid biotype (infecting carnivores of the subfamily Viverrinae). Cross- or spillover infections of one biotype into the host range of the other are thought to occur from time to time. However, very little is known about this phenomenon and its role in the epidemiology of rabies in southern Africa. We have investigated spillover by monoclonal antibody and nucleic acid sequence analysis of a wide range of virus isolates. Although the inverse had been documented, this report constitutes the first evidence of spillover of canid biotype viruses into viverrid hosts. Our genetic analysis was focused specifically on the G-L intergenic region of the virus genome, thought to be a remnant or pseudogene and it was indicated that, with respect to this region of the genome, spillover does not influence the phylogeny of virus isolates.
authors.affiliation: Department of Microbiology and Plant Pathology, Biological and Agricultural Sciences, University of Pretoria, South Africa.
authors.forename: L
authors.initials: L
authors.lastname: Nel
authors.forename: J
authors.initials: J
authors.lastname: Jacobs
authors.forename: J
authors.initials: J
authors.lastname: Jaftha
authors.forename: C
authors.initials: C
authors.lastname: Meredith
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D002335:Carnivora / Q000821:virology*; D006790:Host-Parasite Interactions; D010802:Phylogeny; D011818:Rabies / Q000453:epidemiology* / Q000276:immunology; D011820:Rabies virus / Q000145:classification / Q000235:genetics*; D017421:Sequence Analysis; D013019:South Africa / Q000453:epidemiology
chemical_list: D000911:Antibodies, Monoclonal