Identical T-cell receptor beta chain rearrangements are present in T cells infiltrating the jejunal mucosa of untreated celiac patients.
The intestinal mucosal lesion in celiac disease is characterized by a predominant T-cell infiltration of both epithelium and lamina propria. However, a restricted use of T-cell receptors (TCR) in T lymphocytes infiltrating the jejunal mucosa of celiac patients has not been reported. Based on an immunohistochemical survey of jejunal biopsies from a cohort of untreated celiac patients, we demonstrated a small but significant increase of V beta 8.1/2+ T cells in the lamina propria, but not in the epithelium nor in the peripheral blood. Sequence analysis indicated the existence of a variable degree of clonality of V beta 8+ T cells in the celiac mucosa. More importantly, the recurrence of identical CDR3 regions in some patients was also observed. The altered distribution of V beta 8+ T cells and the presence of identical CDR3 regions in celiac patients, but not in controls was independently confirmed by CDR3 size analysis in a further cohort of patients. These findings suggest that disease-specific variations of the TCRBV8 repertoire are present in the small intestinal mucosa of untreated celiac patients.
Prisco
A
A
International Institute of Genetics and Biophysics, C.N.R., Napoli, Italy.
Troncone
R
R
Mazzarella
G
G
Gianfrani
C
C
Auricchio
S
S
Even
J
J
Tiberio
C
C
Guardiola
J
J
De Berardinis
P
P
D000595:Amino Acid Sequence; D001483:Base Sequence; D002446:Celiac Disease / Q000235:genetics* / Q000276:immunology / Q000473:pathology; D019084:Fluorescent Antibody Technique, Indirect; D015333:Gene Rearrangement, beta-Chain T-Cell Antigen Receptor; D006650:Histocompatibility Testing; D006801:Humans; D007413:Intestinal Mucosa / Q000276:immunology* / Q000473:pathology; D007583:Jejunum / Q000276:immunology* / Q000473:pathology; D008969:Molecular Sequence Data; D016693:Receptors, Antigen, T-Cell, alpha-beta / Q000737:chemistry* / Q000276:immunology; D013601:T-Lymphocytes / Q000276:immunology*
D016693:Receptors, Antigen, T-Cell, alpha-beta; C075784:T cell receptor Vbeta8