Structure-function and pathogenesis studies of Streptococcus pyogenes extracellular cysteine protease.
Replacement of the single cysteine residue (C192) with serine in the Streptococcus pyogenes extracellular cysteine protease (SCP) prevented auto-catalytic processing of the 40-kDa zymogen to the 28-kDa mature form and eliminated proteolytic activity. SCP incubated with human endothelial cells induced a time- and concentration-dependent increase in a 66-kDa gelatinase/type IV collagenase in culture supernatants. Activation of this gelatinase/collagenase may contribute to endothelial cell damage, tissue destruction, and hemodynamic derangement observed in some patients with severe, invasive S. pyogenes infection.
Burns
E H
EH
Department of Pathology, Baylor College of Medicine, Houston, Texas 77030, USA.
Marciel
A M
AM
Musser
J M
JM
D001426:Bacterial Proteins; D001665:Binding Sites / Q000235:genetics; D002478:Cells, Cultured; D017364:Collagenases / Q000378:metabolism; D003546:Cysteine Endopeptidases / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*; D004789:Enzyme Activation; D004792:Enzyme Precursors / Q000378:metabolism; D005098:Exotoxins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D006801:Humans; D020780:Matrix Metalloproteinase 9; D008565:Membrane Proteins; D016297:Mutagenesis, Site-Directed; D013297:Streptococcus pyogenes / Q000201:enzymology* / Q000235:genetics / Q000472:pathogenicity*; D013329:Structure-Activity Relationship; D014774:Virulence
D001426:Bacterial Proteins; D004792:Enzyme Precursors; D005098:Exotoxins; D008565:Membrane Proteins; C117980:SpeA protein, Streptococcus pyogenes; C027951:erythrogenic toxin; D003546:Cysteine Endopeptidases; C042477:streptopain; D017364:Collagenases; D020780:Matrix Metalloproteinase 9