Infection cycle of herpes viruses after photodynamic treatment with methylene blue and light.
Herpes simplex virus type 1 was inactivated by illumination with red light in the presence of low concentrations (1 microM) of methylene blue (MB). In ultrastructural examinations after photodynamic treatment, no significant damage of the viral envelope was observed. The inactivated viruses were able to bind to host cells and to penetrate them. Viral DNA was released within the cells, but DNA replication was completely inhibited by photodynamic treatment. In Southern blots, a degradation of the viral DNA was detected with increasing illumination time. Taken together, these data show that MB/light treatment of herpes viruses gives rise to DNA damage and blocks DNA replication.
Müller-Breitkreutz
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K
DRK-Blutspendedienst Niedersachsen, Springe, Germany.
Mohr
H
H
D000818:Animals; D002522:Chlorocebus aethiops; D004261:DNA Replication / Q000187:drug effects; D018259:Herpesvirus 1, Human / Q000187:drug effects*; D006801:Humans; D008751:Methylene Blue / Q000494:pharmacology*; D010778:Photochemotherapy; D017319:Photosensitizing Agents / Q000494:pharmacology*; D014709:Vero Cells; D014776:Virus Cultivation; D014779:Virus Replication / Q000187:drug effects
D017319:Photosensitizing Agents; D008751:Methylene Blue