[Genomic heterogeneity of hepatitis B virus, genotype A circulating in the metropolitan area of Buenos Aires, Argentina].
HVB DNA was extracted from highly purified Dane particles, from sera of HBV viremic patients, collected in the metropolitan area of Buenos Aires (Argentina). HBV DNA was cloned in pUC18 vector, amplified in Escherichia coli DH5 F'. Plasmids were recovered and analyzed for HBV DNA inserts. Three recombinant plasmids, pHB4, pHB7 and pHB20 were selected, and HBVDNA inserts sequenced. The resulted sequences were incorporated at the GenBank, with the following accession numbers: PHB4P3=U33188; PHB4P5=U33189; PHB7P3=U33190; PHB7P5=U33191 and PHB20=U33190; PHB7P5=U33191 and PHB20=U33187. All belongs to the genotype A, pHB4 and pHB20 have a very close relation in between each other and with L13994 sequence, from North America origin. pHB7 have a significant distance from pHB4 and pHB20 and have a discrete homology with m57633 detected in Philippines. pHB4 shows a mutation at the T 3182-Leu in the preS1 region that change Pro for Leu, this mutation is absent in 125 sequences selected (having a 65% or more of homology) from NCBI by Blast algortm. The sequence of the pre C regions of all three inserts do not show any evidence to belong to the e-or scape mutants. Type A genotypes shows to be common in the area, but a hight degree of divergence have been demonstrate between two circulating strains.
Fernandez
C
C
Departamento de Virus, Instituto Nacional de Microbiología Carlos G. Malbrán.
Mariana
de Torres
R A
RA
D000595:Amino Acid Sequence; D001118:Argentina; D004251:DNA Transposable Elements / Q000235:genetics; D004279:DNA, Viral / Q000032:analysis / Q000235:genetics*; D018740:Genetic Heterogeneity; D005822:Genetic Vectors; D005838:Genotype; D006515:Hepatitis B virus / Q000235:genetics* / Q000302:isolation & purification; D006801:Humans; D008969:Molecular Sequence Data; D014505:Urban Population
D004251:DNA Transposable Elements; D004279:DNA, Viral