Crystallization and characterization of bovine liver glutamate dehydrogenase.
Bovine liver glutamate dehydrogenase has been crystallized as an abortive complex with glutamic acid, NADH, and an inhibitor, GTP. Crystals of this complex were grown using the sitting drop vapor diffusion method with PEG 8000 as the precipitant and diffract to better than 2.5 A resolution. The crystals belong to the space group P2(1) with an entire enzyme hexamer in the crystallographic asymmetric unit. Self-rotation and self-Patterson functions clearly define the orientation and position of this hexameric enzyme.
Peterson
P E
PE
Department of Biological Sciences, Purdue University, West Lafayette, Indiana 47907, USA.
Pierce
J
J
Smith
T J
TJ
D000818:Animals; D002417:Cattle; D003460:Crystallization; D018360:Crystallography, X-Ray; D005969:Glutamate Dehydrogenase / Q000737:chemistry* / Q000302:isolation & purification / Q000378:metabolism; D018698:Glutamic Acid / Q000378:metabolism; D006160:Guanosine Triphosphate / Q000378:metabolism; D008099:Liver / Q000201:enzymology*; D008961:Models, Structural; D009243:NAD / Q000378:metabolism; D011092:Polyethylene Glycols; D011485:Protein Binding; D011487:Protein Conformation; D012399:Rotation
D009243:NAD; D018698:Glutamic Acid; D011092:Polyethylene Glycols; D006160:Guanosine Triphosphate; D005969:Glutamate Dehydrogenase