title: Size-dependent effect of IgA on the IgA Fc receptor (CD89).
abstract: The IgA Fc receptor (FcR; CD89) is expressed on several types of cells of the myeloid cell lineage. We investigated whether different sizes of heat-aggregated IgA (aIgA) bind to CD89 and subsequently induce cellular activation. As a model we used the murine B cell line IIA1.6 transfected with CD89 or IIA1.6 cells transfected with CD89 as well as with the FcR gamma chain to study the binding of IgA to CD89. When these cells expressing CD89 were incubated with monomeric IgA, no significant binding of IgA to the cells was detectable by fluorescence-activated cell sorter analysis; however, incubation of the cells with aggregated IgA resulted in 93 +/- 2% positive cells. Incubation of the cells with different sizes of IgA-containing aggregates revealed optimal binding with aggregates containing five to six molecules of IgA per aggregate. No difference was observed between the binding to CD89 of both IgA1- or IgA2-containing aggregates. Furthermore, the binding of aIgA was found to be CD89-specific, since the binding of IgA was completely inhibited by the CD89-specific monoclonal antibody My43 and no detectable binding occurred to the IIA1.6 parent cell line. Activation studies using interleukin-2 (IL-2) production as a marker, showed that the FcR gamma chain is necessary to induce cellular activation. Only cells transfected with both CD89 and the FcR gamma chain (CD89+/gamma +) enhance the IL-2 production 10-12-fold upon stimulation with aggregates of IgA. Furthermore, triggering of CD89 only results in increase of intracellular calcium concentration ([Ca2+]i) in cells co-expressing FcR gamma chain. Mutation of the tyrosine residues in the FcR gamma chain immunoreceptor tyrosine-based activation motif of the FcR gamma chain abolishes this increase in [Ca2+]i, indicating association and involvement of the FcR gamma chain in CD89-mediated signaling.
authors.affiliation: Department of Nephrology, Leiden University Hospital, The Netherlands.
authors.forename: T J
authors.initials: TJ
authors.lastname: Reterink
authors.forename: G
authors.initials: G
authors.lastname: van Zandbergen
authors.forename: M
authors.initials: M
authors.lastname: van Egmond
authors.forename: N
authors.initials: N
authors.lastname: Klar-Mohamad
authors.forename: C H
authors.initials: CH
authors.lastname: Morton
authors.forename: J G
authors.initials: JG
authors.lastname: van de Winkel
authors.forename: M R
authors.initials: MR
authors.lastname: Daha
mesh_terms: D019943:Amino Acid Substitution; D000818:Animals; D001665:Binding Sites; D002118:Calcium / Q000502:physiology; D006801:Humans; D007070:Immunoglobulin A / Q000737:chemistry* / Q000276:immunology; D007376:Interleukin-2 / Q000502:physiology; D051379:Mice; D011961:Receptors, Fc / Q000737:chemistry / Q000502:physiology*; D017452:Receptors, IgG / Q000737:chemistry / Q000502:physiology*; D011994:Recombinant Proteins; D015398:Signal Transduction; D013329:Structure-Activity Relationship; D014443:Tyrosine / Q000737:chemistry
chemical_list: C045281:IgA receptor; D007070:Immunoglobulin A; D007376:Interleukin-2; D011961:Receptors, Fc; D017452:Receptors, IgG; D011994:Recombinant Proteins; C034639:polymeric IgA; D014443:Tyrosine; D002118:Calcium