Relationship between spermatozoan lipid composition and fertility during aging of chickens.
Changes in the proportions of the various lipid components in spermatozoa were investigated throughout the reproductive period (24-72 wk of age) of male chickens. Sperm motility and in vivo fertility were also measured, and correlation coefficients with the lipid values were determined. The proportion of total phospholipid (PL) increased to reach a maximum value at 39 wk and decreased significantly thereafter. The relative content of free cholesterol and triacylglycerols showed no change in spermatozoa during aging or in relation to fertility values; free fatty acids and cholesterol esters increased continuously with age. Of the various PL classes, phosphatidylserine and phosphatidylcholine displayed a pattern of changes with age positively and negatively, respectively, in relation to the changes of fertility. The proportion of phosphatidylethanolamine had significantly decreased by the end of the reproductive period. The proportions of C16:0, C18:0, and C18:1n-9 within the PL of the spermatozoa increased with age, and those of C20:4n-6, C22:4n-6, and C22:6n-3 decreased. Positive correlations were found between fertility and total PLs, phosphatidylserine, and PL-bound C20:4n-6 and C22:4n-6; a negative correlation was found between fertility and phosphatidylcholine. Motility was positively correlated with the level of PL and negatively with that of free cholesterol; it was also positively correlated with the levels of C22:4n-6 and C22:6n-3 and negatively with those of C16:0, C18:0, and C18:1n-9. The results suggest that the lipid and fatty acid compositions of spermatozoa may be important predictors of fertility.
Cerolini
S
S
Istituto per la Difesa e la Valorizzazione del Germoplasma Animale, Consiglio Nazionale delle Ricerche, Milano, Italy. s.cerolini@au.sac.ac.uk
Kelso
K A
KA
Noble
R C
RC
Speake
B K
BK
Pizzi
F
F
Cavalchini
L G
LG
D000375:Aging / Q000502:physiology*; D000818:Animals; D002645:Chickens / Q000502:physiology*; D002784:Cholesterol / Q000378:metabolism; D005227:Fatty Acids / Q000378:metabolism; D005298:Fertility / Q000502:physiology*; D007315:Insemination, Artificial; D050356:Lipid Metabolism; D008297:Male; D010713:Phosphatidylcholines / Q000378:metabolism; D010718:Phosphatidylserines / Q000378:metabolism; D013081:Sperm Motility / Q000502:physiology; D013094:Spermatozoa / Q000378:metabolism* / Q000502:physiology*; D014280:Triglycerides / Q000378:metabolism
D005227:Fatty Acids; D010713:Phosphatidylcholines; D010718:Phosphatidylserines; D014280:Triglycerides; D002784:Cholesterol