Restriction endonucleases in clinical isolates of Shigella spp.
Thirteen restriction endonuclease-containing strains were isolated from a collection of 186 clinical isolates of Shigella spp. Among these, eight and five isolates carried isoschizomers of EcoRII and NciI, respectively. The former restriction-modification (R-M) system was homologous to that of EcoRII and was located on plasmids with sizes of 46.6 or 55.6 kb. Isolates producing NciI isoschizomers contained a 5.7-kb non-transferable plasmid. Together with antimicrobial susceptibility tests and plasmid profile studies, it is concluded that these two R-M systems are not widely spread but confined to strains with similar antibiotic resistance and plasmid profile.
Lee
K F
KF
Department of Biochemistry, Chinese University of Hong Kong, Hong Kong.
Ling
J M
JM
Kam
K M
KM
Clark
D R
DR
Shaw
P C
PC
D000900:Anti-Bacterial Agents / Q000494:pharmacology; D004269:DNA, Bacterial / Q000032:analysis* / Q000378:metabolism; D004352:Drug Resistance, Microbial; D004720:Endonucleases / Q000378:metabolism*; D008826:Microbial Sensitivity Tests; D010957:Plasmids / Q000032:analysis* / Q000378:metabolism; D012760:Shigella / Q000187:drug effects / Q000201:enzymology* / Q000276:immunology
D000900:Anti-Bacterial Agents; D004269:DNA, Bacterial; D004720:Endonucleases