title: Regulation of phagosomal acidification. Differential targeting of Na+/H+ exchangers, Na+/K+-ATPases, and vacuolar-type H+-atpases.
abstract: Vacuolar-type (V) ATPases are thought to be the main determinant of phagosomal acidification. In phagosomes containing mycobacteria, which ostensibly impair the delivery of V-ATPases to the phagosomal membrane, the pH would be expected to be near neutral. This prediction was tested by microfluorescence ratio imaging using macrophages from mice susceptible to mycobacterial infection. Although less acidic than their counterparts containing dead bacteria, phagosomes containing live Mycobacteria bovis were nearly 1 pH unit more acidic than the cytosol, suggesting the existence of alternate H+ transport mechanisms. We therefore investigated whether Na+/H+ exchange (NHE) contributes to phagosomal acidification. Immunoblotting, reverse transcriptase-polymerase chain reaction, and pharmacological studies indicated that NHE1 is the predominant isoform of the exchanger in macrophages. Fractionation revealed that NHE1 is incorporated into the phagosomal membrane, and measurements of pH indicated that it is functional in this location. Nevertheless, acidification of the lumen of phagosomes containing either latex beads or live M. bovis was insensitive to (3-methylsulfonyl-4-piperidinobenzoyl)-guanidine methanesulfonate, a potent inhibitor of NHE1. This may have been due to the absence of an appropriate lumen to cytosol Na+ gradient, because the phagosomal membrane was found to be devoid of Na+/K+ pumps. Unexpectedly, the acidification of M. bovis phagosomes was fully reversed by specific inhibitors of the vacuolar H+-ATPase, suggesting that ATPases are present only transiently or in reduced quantities in the phagosomal membrane. Alternatively, acid equivalents accumulated in endosomes by V-ATPases may be delivered to the mycobacterial phagosome by carrier vesicles devoid of ATPases.
authors.affiliation: Division of Cell Biology, The Hospital for Sick Children, Toronto Hospital, University of Toronto, Toronto M5G 1X8, Canada.
authors.forename: D J
authors.initials: DJ
authors.lastname: Hackam
authors.forename: O D
authors.initials: OD
authors.lastname: Rotstein
authors.forename: W J
authors.initials: WJ
authors.lastname: Zhang
authors.forename: N
authors.initials: N
authors.lastname: Demaurex
authors.forename: M
authors.initials: M
authors.lastname: Woodside
authors.forename: O
authors.initials: O
authors.lastname: Tsai
authors.forename: S
authors.initials: S
authors.lastname: Grinstein
mesh_terms: D000818:Animals; D002460:Cell Line; D006863:Hydrogen-Ion Concentration; D051379:Mice; D008956:Models, Chemical; D009161:Mycobacterium / Q000166:cytology / Q000201:enzymology; D010588:Phagosomes / Q000201:enzymology*; D017494:Proton Pumps / Q000378:metabolism*; D006180:Proton-Translocating ATPases / Q000378:metabolism*; D017923:Sodium-Hydrogen Exchangers / Q000378:metabolism*; D000254:Sodium-Potassium-Exchanging ATPase / Q000378:metabolism*; D025262:Vacuolar Proton-Translocating ATPases
chemical_list: D017494:Proton Pumps; D017923:Sodium-Hydrogen Exchangers; C069139:growth factor-activatable Na-H exchanger NHE-1; D025262:Vacuolar Proton-Translocating ATPases; D006180:Proton-Translocating ATPases; D000254:Sodium-Potassium-Exchanging ATPase