Attenuation of renomedullary phospholipase C isozyme, PLC-delta 1, in spontaneously hypertensive rats.
The distributional patterns of PLC isozymes within the kidney were investigated using spontaneously hypertensive rats (SHRs) and normotensive Wistar-Kyoto (WKY) rats at 4 and 12 weeks of age. PLC-beta 1, PLC-beta 3 and PLC-delta 1 quantified by Western blot analysis, were present in the highest concentrations in the inner medulla of rats at both 4 and 12 weeks of age. On the other hand, PLC-beta 4, PLC-gamma 1 and PLC-gamma 2 were distributed almost equally among the regions for the rats of both ages. When compared with WKY rats at 12 weeks of age, the amounts of PLC-beta 1, PLC-beta 3, PLC-gamma 1, PLC-gamma 2, and PLC-delta 1 in the inner medulla of SHRs were significantly lower, and the amount of PLC-delta 1 in the inner stripe of the outer medulla was also significantly lower. Even at the prehypertensive stage at 4 weeks of age, the inner medullary concentration of PLC-delta 1 was significantly lower in SHRs than WKY rats. These results suggest that PLC-delta 1 would play an important role in the development of hypertension.
Lee
K H
KH
Department of Pharmacology, Catholic University Medical College, Seoul, Korea.
Cho
Y J
YJ
Cha
S H
SH
Endou
H
H
D000818:Animals; D006973:Hypertension / Q000201:enzymology; D015151:Immunoblotting; D007527:Isoenzymes / Q000172:deficiency / Q000378:metabolism*; D007672:Kidney Cortex / Q000201:enzymology / Q000276:immunology; D007679:Kidney Medulla / Q000201:enzymology* / Q000276:immunology; D008297:Male; D009928:Organ Specificity; D009994:Osmolar Concentration; D054799:Phospholipase C beta; D054803:Phospholipase C delta; D051966:Phospholipase C gamma; D051381:Rats; D011918:Rats, Inbred SHR; D011921:Rats, Inbred WKY; D010738:Type C Phospholipases / Q000172:deficiency / Q000378:metabolism*
D007527:Isoenzymes; D010738:Type C Phospholipases; D054799:Phospholipase C beta; D054803:Phospholipase C delta; D051966:Phospholipase C gamma