The resolution of DNA fragments in capillary electrophoresis in replaceable agarose gels.
The resolution of DNA restriction fragments in CE in replaceable agarose gels was investigated as a function of the concentration and pH of Tris-acetate ethylenediaminetetraacetic acid (EDTA) buffer both at constant migration time and at constant field strength. The resolution increased for all fragments when the buffer concentration was increased from 40 to 200 mM and the pH was lowered from 8.5 to 7.5. The resolution was lower in a Tris-borate-EDTA buffer in this pH range and lowest at pH 7.5.
Palm
A
A
Department of Biochemistry, Uppsala University, Sweden.
Hjertén
S
S
D002021:Buffers; D004247:DNA / Q000737:chemistry*; D004492:Edetic Acid; D004587:Electrophoresis, Agar Gel / Q000379:methods; D019075:Electrophoresis, Capillary / Q000379:methods; D006863:Hydrogen-Ion Concentration; D007202:Indicators and Reagents; D009838:Oligodeoxyribonucleotides / Q000302:isolation & purification*; D015203:Reproducibility of Results; D015183:Restriction Mapping; D012680:Sensitivity and Specificity
D002021:Buffers; D007202:Indicators and Reagents; D009838:Oligodeoxyribonucleotides; D004247:DNA; D004492:Edetic Acid