title: In vitro susceptibility studies and detection of vancomycin resistance genes in clinical isolates of enterococci in Nagasaki, Japan.
abstract: Glycopeptide resistance in enterococci is now a cause of clinical concern in the United States and Europe. However, details of vancomycin resistance in enterococci in Japan have been unknown. We measured minimum inhibitory concentrations (MICs) of various antimicrobial agents for a total of 218 clinical strains of enterococci isolated in our hospital in 1995-6 in addition to 15 strains with known genotypic markers of resistance. We also screened vancomycin resistance genes using a single step multiplex-PCR. In clinical isolates, only two strains of Enterococcus gallinarum were of intermediate resistance to vancomycin (MIC, 8 micrograms/ml), while the others were all susceptible. Glycopeptides (vancomycin and teicoplanin) and streptogramins (RP 58500 and RPR 106972) showed potent antimicrobial effects for the isolates. In addition, ampicillin was also potent for Enterococcus faecalis, while ampicillin, minocycline and gentamicin were potent for Enterococcus avium. No vanA or vanB genes were detected, while vanC1 and vanC23 genes were detected from two and four strains, respectively. Our results suggest that incidence of VRE in Japan may be estimated as still very low at this time.
authors.affiliation: Department of Laboratory Medicine, Nagasaki University School of Medicine, Japan.
authors.forename: Y
authors.initials: Y
authors.lastname: Hirakata
authors.forename: T
authors.initials: T
authors.lastname: Yamaguchi
authors.forename: K
authors.initials: K
authors.lastname: Izumikawa
authors.forename: J
authors.initials: J
authors.lastname: Matsuda
authors.forename: K
authors.initials: K
authors.lastname: Tomono
authors.forename: M
authors.initials: M
authors.lastname: Kaku
authors.forename: H
authors.initials: H
authors.lastname: Koga
authors.forename: Y
authors.initials: Y
authors.lastname: Yamada
authors.forename: S
authors.initials: S
authors.lastname: Kohno
authors.forename: S
authors.initials: S
authors.lastname: Kamihira
mesh_terms: D000900:Anti-Bacterial Agents; D003428:Cross Infection / Q000382:microbiology*; D004269:DNA, Bacterial / Q000235:genetics*; D004352:Drug Resistance, Microbial; D016983:Enterococcus / Q000145:classification / Q000235:genetics*; D005838:Genotype; D016908:Gram-Positive Bacterial Infections / Q000382:microbiology*; D006801:Humans; D015994:Incidence; D017053:Infection Control; D007564:Japan; D008826:Microbial Sensitivity Tests; D016133:Polymerase Chain Reaction; D014504:Urban Health; D014640:Vancomycin
chemical_list: D000900:Anti-Bacterial Agents; D004269:DNA, Bacterial; D014640:Vancomycin