[Use of Escherichia coli recF mutant strains for analyzing the genotoxicity of complex platinum compounds].
The bioluminescence method of assessing SOS response in Escherichia coli cells was applied to test the genotoxicity of five complex platinum compounds: cis-diamminedichloroplatinum, cycloplatam, trans-diamminedichloroplatinum, and two trans-binuclear complexes. Strains AB1157 (pPLS-1) and JC9239 (pPLS-1), a variant of AB1157 carrying the recF143 mutation, were used in the test procedure. SOS response in JC9239 cells was shown to be induced by significantly lower concentrations of cis-DDP and cycloplatam than in AB1157 cells. A drastic increase in cis-DDP toxicity for the JC9239 strain was shown. However, the presence of the recF mutation in JC9239 cells retarded the SOS response induced by trans-platinum compounds. The results obtained indicated that the E. coli recF system plays an essential role in repair and utilization of cis-DDP-DNA adducts.
Ptitsyn
L R
LR
Riazanova
L A
LA
Ivanov
V B
VB
D001426:Bacterial Proteins / Q000235:genetics*; D018736:DNA Adducts; D004260:DNA Repair; D004268:DNA-Binding Proteins / Q000235:genetics*; D004926:Escherichia coli / Q000235:genetics*; D029968:Escherichia coli Proteins; D009152:Mutagenicity Tests; D009153:Mutagens / Q000633:toxicity*; D009154:Mutation; D009944:Organoplatinum Compounds / Q000633:toxicity*; D013014:SOS Response, Genetics
D001426:Bacterial Proteins; D018736:DNA Adducts; D004268:DNA-Binding Proteins; D029968:Escherichia coli Proteins; D009153:Mutagens; D009944:Organoplatinum Compounds; C489340:recF protein, E coli; C066218:recF protein, Bacteria