Comparison of the thiamine level in blood and erythrocyte transketolase activity in hemodialyzed and nondialyzed patients during recombinant human erythropoietin therapy.
Thiamine and erythrocyte transketolase activity (ETKA) disturbances in end-stage renal disease are caused mainly by uremia and dialysis treatment. We examined whether recombinant human erythropoietin (rhEPO) can correct these abnormalities in uremic patients. Thirteen hemodialysis (HD) and 12 nondialyzed (ND) anemic patients showed decreased free and total thiamine levels in plasma and in erythrocytes and decreased ETKA when compared to 20 healthy subjects. Thiamine blood levels (mumol/l) were determined using a fluorimetric technique, and ETKA (mumol/l per minute) was assessed with a photocolorimetric method. Over 20 weeks of study, rhEPO was given intravenously for 8 weeks at 50 Ul/kg body weight (BW) three times a week, and subcutaneously for 4 weeks at 25 Ul/kg BW, twice a week, and for the last 8 weeks at 25 Ul/kg BW once a week. The correction of anemia was associated with an increase in plasma thiamine and erythrocyte total thiamine as well as ETKA in HD patients and with an increase in erythrocyte total thiamine in ND patients only during the period of intravenous infusions.
Pietrzak
I
I
Department of Nephrology, University of Medical Sciences, Poznań, Poland.
Baczyk
K
K
D000328:Adult; D001798:Blood Proteins / Q000378:metabolism; D003131:Combined Modality Therapy; D004912:Erythrocytes / Q000201:enzymology*; D004921:Erythropoietin / Q000627:therapeutic use*; D005069:Evaluation Studies as Topic; D005260:Female; D006454:Hemoglobins / Q000378:metabolism; D006801:Humans; D008297:Male; D008875:Middle Aged; D011994:Recombinant Proteins; D006435:Renal Dialysis; D013831:Thiamine / Q000097:blood*; D014174:Transketolase / Q000097:blood*
D001798:Blood Proteins; D006454:Hemoglobins; D011994:Recombinant Proteins; D004921:Erythropoietin; D014174:Transketolase; D013831:Thiamine