title: Genome organization of human 48-kDa oligosaccharyltransferase (DDOST).
abstract: The enzyme oligosaccharyltransferase (dolichyl-diphosphooligosaccharide-protein glycosyltransferase; EC 2. 4.1.119) (DDOST) catalyzes the transfer of a high-mannose oligosaccharide (GlcNac2Man9Glc3) from a dolichol-linked oligosaccharide donor (dolichol-P-GlcNac2Man9Glc3) onto the asparagine acceptor site within an Asn-X-Ser/Thr consensus motif in nascent polypeptide chains across the membrane of the endoplasmic reticulum. We isolated mouse and human DDOST cDNAs from retinoic acid-treated mouse P19 EC cells and human NT-2 cells, respectively. DDOST mRNA is expressed intensely in heart and pancreas, but at lower levels in brain. Here we show that the human DDOST 48-kDa subunit gene (HGMW-approved symbol DDOST) is organized into 11 exons expanding about 9 kb. This DDOST subunit gene is localized on chromosome 1p36.1 by fluorescence in situ hybridization analysis.
authors.affiliation: Department of Pediatrics, Jichi Medical School, Minamikawachi-machi, Kawachi-gun, Tochigi, 329-04, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Yamagata
authors.forename: T
authors.initials: T
authors.lastname: Tsuru
authors.forename: M Y
authors.initials: MY
authors.lastname: Momoi
authors.forename: K
authors.initials: K
authors.lastname: Suwa
authors.forename: Y
authors.initials: Y
authors.lastname: Nozaki
authors.forename: T
authors.initials: T
authors.lastname: Mukasa
authors.forename: H
authors.initials: H
authors.lastname: Ohashi
authors.forename: Y
authors.initials: Y
authors.lastname: Fukushima
authors.forename: T
authors.initials: T
authors.lastname: Momoi
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D002878:Chromosomes, Human, Pair 1; D018076:DNA, Complementary; D006602:Hexosyltransferases; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D008565:Membrane Proteins; D051379:Mice; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction / Q000379:methods; D012326:RNA Splicing; D017386:Sequence Homology, Amino Acid; D014018:Tissue Distribution; D014166:Transferases / Q000235:genetics*
chemical_list: D018076:DNA, Complementary; D008565:Membrane Proteins; D014166:Transferases; D006602:Hexosyltransferases; C025620:dolichyl-diphosphooligosaccharide - protein glycotransferase