title: The effect of short- vs. long-term platelet-activating factor exposure on mouse preimplantation embryo development.
abstract: Preimplantation mouse embryos (n = 1540) were cultured in the presence of platelet-activating factor (PAF) (10(-7)- 10(-14) mol/l) to the hatched blastocyst stage. A dose-dependent negative correlation (-0.75783) relationship between blastocysts and the concentration of PAF was statistically significantly different (p < 0.001). Long-term but not short-term PAF exposure is detrimental to preimplantation Swiss Webster mouse embryos. Short-term PAF (10(-9) mol/l) exposure was found significantly (p < 0.05) to reduce blastocoel diameter. The effect of PAF during preimplantation development may be genotype dependent and be affected by the culture conditions.
authors.affiliation: Department of Obstetrics and Gynecology, Medical University of South Carolina, Charleston 29425, USA.
authors.forename: G
authors.initials: G
authors.lastname: Radonjic-Lazovic
authors.forename: W E
authors.initials: WE
authors.lastname: Roudebush
mesh_terms: D000818:Animals; D001755:Blastocyst / Q000502:physiology*; D046508:Culture Techniques; D004305:Dose-Response Relationship, Drug; D047108:Embryonic Development; D005260:Female; D051379:Mice; D010972:Platelet Activating Factor / Q000008:administration & dosage / Q000494:pharmacology*; D011247:Pregnancy
chemical_list: D010972:Platelet Activating Factor