title: Chromatin structure of the Saccharomyces cerevisiae DNA topoisomerase I promoter in different growth phases.
abstract: We have determined the chromatin organization of the Saccharomyces cerevisiae DNA topoisomerase I promoter. Three nucleosomal core particles have been mapped at nucleotide level over the promoter region, encompassing the presumptive TATA sequence and the two RNA initiation sites; the most upstream nucleosome particle forms on to a 29 bp-long poly(dA-dT) element. This simple organization remains constant throughout both the logarithmic and the linear phase of growth, with the exception of an increased accessibility to micrococcal nuclease of the nucleosome covering the TATA box and the RNA initiation sites during the diauxic shift (the switching from the fermentative to the respiratory metabolism) in parallel with an increase of the DNA topoisomerase I mRNA. In addition, a strong disorganization of the bulk chromatin structure in the late stationary phase is also reported.
authors.affiliation: Fondazione Istituto Pasteur-Fondazione Cenci-Bolognetti, c/o Dipartimento di Genetica e Biologia Molecolare, Università 'La Sapienza', P.le A. Moro 5, 00185 Rome, Italy.
authors.forename: L
authors.initials: L
authors.lastname: Rubbi
authors.forename: G
authors.initials: G
authors.lastname: Camilloni
authors.forename: M
authors.initials: M
authors.lastname: Caserta
authors.forename: E
authors.initials: E
authors.lastname: Di Mauro
authors.forename: S
authors.initials: S
authors.lastname: Venditti
mesh_terms: D002843:Chromatin / Q000235:genetics*; D004264:DNA Topoisomerases, Type I / Q000235:genetics*; D015971:Gene Expression Regulation, Enzymologic; D009707:Nucleosomes; D011401:Promoter Regions, Genetic / Q000235:genetics*; D012331:RNA, Fungal / Q000032:analysis; D012333:RNA, Messenger / Q000032:analysis; D012441:Saccharomyces cerevisiae / Q000201:enzymology / Q000235:genetics* / Q000254:growth & development
chemical_list: D002843:Chromatin; D009707:Nucleosomes; D012331:RNA, Fungal; D012333:RNA, Messenger; D004264:DNA Topoisomerases, Type I