title: Chemical characterization of the predominant proteins secreted by mouse seminal vesicles.
abstract: Mouse seminal vesicles secrete four major protein components with estimated molecular masses of 95, 38, 17, and 16 kDa. Amino acid sequencing revealed that the 95-kDa component represents a protein with an unknown structure, while the 38-kDa component was identified as semenoclotin, the 17-kDa component as seminal-vesicle-secreted protein IV, and the 16-kDa component as seminal-vesicle-secreted protein V. Semenoclotin and the 95-kDa component were readily cross-linked by transglutaminase, suggesting that the two proteins are involved in the formation of the mouse copulatory plug. Treatment of mouse seminal vesicle fluid with human prostate-specific antigen rapidly degraded semenoclotin, indicating a structural resemblance of this protein to human semenogelins, despite the vast difference in primary structure. As previously reported for other seminal-vesicle-secreted proteins, the semenoclotin transcripts are shown to be under androgen control.
authors.affiliation: Lund University, Department of Laboratory Medicine, University Hospital, Malmö, Sweden. ake.lundwall@klkemi.mas.lu.se
authors.forename: A
authors.initials: A
authors.lastname: Lundwall
authors.forename: A
authors.initials: A
authors.lastname: Peter
authors.forename: J
authors.initials: J
authors.lastname: Lövgren
authors.forename: H
authors.initials: H
authors.lastname: Lilja
authors.forename: J
authors.initials: J
authors.lastname: Malm
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D003432:Cross-Linking Reagents; D012739:Gonadal Steroid Hormones / Q000737:chemistry / Q000235:genetics; D006801:Humans; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008970:Molecular Weight; D011499:Protein Processing, Post-Translational; D011506:Proteins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D029607:Seminal Vesicle Secretory Proteins; D012669:Seminal Vesicles / Q000737:chemistry / Q000187:drug effects / Q000378:metabolism*; D013739:Testosterone / Q000494:pharmacology; D011503:Transglutaminases
chemical_list: D003432:Cross-Linking Reagents; D012739:Gonadal Steroid Hormones; D011506:Proteins; D012333:RNA, Messenger; D029607:Seminal Vesicle Secretory Proteins; C098433:semenoclotin; C424482:seminal vesicle-specific antigen; D013739:Testosterone; D011503:Transglutaminases