Quantitation of luciferase reporter mRNA by in vitro translation of total cellular RNA is faster and more sensitive than northern blotting and primer extension.
Ruediger
R
R
Department of Pathology, University of California at San Diego, La Jolla, California, 92093-0612, USA.rruediger@ucsd.edu
Walter
G
G
D000818:Animals; D015152:Blotting, Northern; D019556:COS Cells; D017931:DNA Primers / Q000378:metabolism*; D017930:Genes, Reporter; D015497:HIV-1 / Q000235:genetics; D008156:Luciferases / Q000235:genetics*; D014176:Protein Biosynthesis; D012313:RNA / Q000378:metabolism*; D012333:RNA, Messenger / Q000032:analysis*; D012091:Repetitive Sequences, Nucleic Acid; D014162:Transfection
D017931:DNA Primers; D012333:RNA, Messenger; D012313:RNA; D008156:Luciferases