Effects of 5-(N-aminohexyl)carbamoyl-2'-deoxyuridine on endonuclease stability and the ability of oligodeoxynucleotide to activate RNase H.
To evaluate an endonuclease resistance property of oligodeoxynucleotides (ODNs) containing 5-(N-aminohexyl)carbamoyl-2'-deoxyuridines (Hs) and to elucidate whether a duplex consisting of the ODN analogue and its complementary RNA induces RNase H activity, the ODNs containing the deoxyuridine analogues, Hs, at intervals of one, two, three, four and five natural nucleosides were synthesized. From partial hydrolysis of these ODNs with nuclease S1 (an endonuclease), it was found that the ODNs became more stable towards nucleolytic hydrolysis by the enzyme as the number of H increased. Furthermore, to examine whether the duplexes composed of the ODNs containing Hs and their complementary RNAs are substrates for RNase H or not, the duplexes of these ODNs and their complementary RNA strands were treated with Escherichia coliRNase H. It was found that cleavage of the RNA strands by the enzyme was kinetically affected by the introduction of Hs into the duplexes.
Ueno
Y
Y
Faculty of Pharmaceutical Sciences, Hokkaido University, Kita-12, Nishi-6, Kita-ku, Sapporo 060, Japan.
Kumagai
I
I
Haginoya
N
N
Matsuda
A
A
D001483:Base Sequence; D002942:Circular Dichroism; D003857:Deoxyuridine / Q000031:analogs & derivatives* / Q000494:pharmacology; D004591:Electrophoresis, Polyacrylamide Gel; D004789:Enzyme Activation / Q000187:drug effects; D004795:Enzyme Stability / Q000187:drug effects; D004926:Escherichia coli / Q000201:enzymology; D006868:Hydrolysis; D007700:Kinetics; D009682:Magnetic Resonance Spectroscopy; D016376:Oligonucleotides, Antisense / Q000235:genetics / Q000494:pharmacology*; D018075:RNA, Complementary / Q000378:metabolism; D016914:Ribonuclease H / Q000378:metabolism*; D015719:Single-Strand Specific DNA and RNA Endonucleases / Q000378:metabolism*; D016339:Spectrometry, Mass, Fast Atom Bombardment; D013379:Substrate Specificity; D013816:Thermodynamics
C108707:5-(N-aminohexyl)carbamoyl-2'-deoxyuridine; D016376:Oligonucleotides, Antisense; D018075:RNA, Complementary; D016914:Ribonuclease H; D015719:Single-Strand Specific DNA and RNA Endonucleases; D003857:Deoxyuridine