Lipid peroxidation and antioxidant enzymes in livers and brains of aged rats.
The contribution of free radical damage to aging in the liver and brain is still controversial. There have also been several reports with conflicting results on the antioxidant system during aging. In this study, we investigated endogenous lipid peroxide levels in the liver and brain tissues of rats aged 6 and 22 months together with ascorbate-induced lipid peroxidation. Also, superoxide dismutase (SOD) and glutathione peroxidase (GPx), the main antioxidant enzymes were assayed. Although ascorbate-induced lipid peroxide levels remained unchanged in aged animals, hepatic lipid peroxidation was seen to be elevated. Glutathione (GSH) content was found to be decreased, but SOD and GPx remained unchanged. No apparent difference in any parameter in brain tissues was observed in the old group.
Doğru-Abbasoğlu
S
S
Department of Biochemistry, Istanbul Faculty of Medicine, University of Istanbul, Turkey.
Tamer-Toptani
S
S
Uğurnal
B
B
Koçak-Toker
N
N
Aykaç-Toker
G
G
Uysal
M
M
D000375:Aging / Q000378:metabolism; D000818:Animals; D000975:Antioxidants / Q000378:metabolism*; D001205:Ascorbic Acid / Q000494:pharmacology; D001921:Brain / Q000187:drug effects / Q000201:enzymology / Q000378:metabolism*; D005979:Glutathione Peroxidase / Q000378:metabolism*; D015227:Lipid Peroxidation / Q000187:drug effects / Q000502:physiology*; D008099:Liver / Q000187:drug effects / Q000201:enzymology / Q000378:metabolism*; D008297:Male; D051381:Rats; D017208:Rats, Wistar; D013482:Superoxide Dismutase / Q000378:metabolism*
D000975:Antioxidants; D005979:Glutathione Peroxidase; D013482:Superoxide Dismutase; D001205:Ascorbic Acid