High-performance liquid chromatographic determination of thiamine diphosphate in erythrocytes using internal standard methodology.
A high-performance liquid chromatography method for the determination of thiamine diphosphate (vitamin B1) in erythrocytes is presented. The method is robust, accurate and reproducible and due to the use of acetylaneurine as an internal standard, offers advantages over previous methods.
Lynch
P L
PL
Department of Clinical Biochemistry, The Royal Group of Hospitals, Belfast, UK.
Trimble
E R
ER
Young
I S
IS
D002851:Chromatography, High Pressure Liquid; D004912:Erythrocytes / Q000737:chemistry*; D006801:Humans; D012015:Reference Standards; D015203:Reproducibility of Results; D013831:Thiamine / Q000031:analogs & derivatives / Q000097:blood; D013835:Thiamine Pyrophosphate / Q000097:blood*
C109997:acetylaneurine; D013835:Thiamine Pyrophosphate; D013831:Thiamine