title: Isolation and characterization of cytotoxic diterpenoid isomers from propolis.
abstract: The methanol extract of Brazilian propolis was fractionated by HPLC, based on HuH13 (human hepatocellular carcinoma) cell cytotoxicity assay. Two isomers of diterpenoid with a molecular formula of C20H30O3 (MW: 318.46) were isolated. The structures of these colorless compounds were determined as clerodane diterpenoids (I, 15-oxo-3, 13Z-kolavadien-17-oic acid; II, 15-oxo-3Z, 13E-kolavadien-17-oic acid).
authors.affiliation: National Institute of Health, Tokyo, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Matsuno
authors.forename: Y
authors.initials: Y
authors.lastname: Matsumoto
authors.forename: M
authors.initials: M
authors.lastname: Saito
authors.forename: J
authors.initials: J
authors.lastname: Morikawa
mesh_terms: D001938:Brazil; D006528:Carcinoma, Hepatocellular; D002470:Cell Survival / Q000187:drug effects*; D002851:Chromatography, High Pressure Liquid; D004224:Diterpenes / Q000737:chemistry* / Q000302:isolation & purification / Q000633:toxicity; D006367:HeLa Cells; D006801:Humans; D007624:KB Cells; D008113:Liver Neoplasms; D015394:Molecular Structure; D011429:Propolis / Q000737:chemistry* / Q000633:toxicity; D014407:Tumor Cells, Cultured
chemical_list: C110045:13-symphyoreticulic acid; D004224:Diterpenes; D011429:Propolis