Propagation and titration of murine cytomegalovirus in a continuous bone marrow-derived stromal cell line (M2-10B4).
Murine cytomegalovirus (MCMV) can only be propagated effectively in mouse embryo fibroblast (MEF) cells. We demonstrate that MCMV replicates significantly better in M2-10B4 cells, a continuous line of murine bone marrow stromal cells. M2-10B4 cells were also comparable to MEF cells for detection of small amounts of MCMV reactivating from latently infected spleen explants. M2-10B4 cells will be very useful for studies of MCMV pathogenesis.
Lutarewych
M A
MA
Department of Medicine, University of Minnesota Medical School, Minneapolis 55455, USA.
Quirk
M R
MR
Kringstad
B A
BA
Li
W
W
Verfaillie
C M
CM
Jordan
M C
MC
D000818:Animals; D001854:Bone Marrow Cells / Q000821:virology*; D002460:Cell Line; D003588:Cytopathogenic Effect, Viral; D005260:Female; D006566:Herpesviridae Infections / Q000821:virology; D051379:Mice; D008807:Mice, Inbred BALB C; D008809:Mice, Inbred C3H; D008810:Mice, Inbred C57BL; D018146:Muromegalovirus / Q000254:growth & development* / Q000302:isolation & purification / Q000502:physiology; D009924:Organ Culture Techniques; D013154:Spleen / Q000821:virology; D017154:Stromal Cells / Q000821:virology; D010948:Viral Plaque Assay; D017735:Virus Latency