In vitro studies on transport and metabolism of short-chain fatty acids in pig hindgut.
An analytical method based on alkaline freeze drying, ultracentrifugation, and quantitative gas chromatography was established to differentiate between mucosal uptake, tissue accumulation, and serosal release of SCFA in pig hindgut. It was shown that serosal release of SCFA was substantially lower than mucosal uptake and tissue accumulation, indicating substantial degradation and/or metabolism during transepithelial movement.
Breves
G
G
Department of Veterinary Physiology, Justus-Liebig-University Giessen, Germany.
Krumscheid
R
R
D000818:Animals; D001692:Biological Transport / Q000502:physiology; D002849:Chromatography, Gas; D005232:Fatty Acids, Volatile / Q000378:metabolism*; D005612:Freeze Drying; D007413:Intestinal Mucosa / Q000378:metabolism*; D013552:Swine / Q000378:metabolism*
D005232:Fatty Acids, Volatile