Adsorptive stripping voltammetry of bleomycin.
In 0.05 M H2SO4 solution, two reductive peaks, P1 and P2, of bleomycin were obtained. The peak potentials E(P1) and E(P2) were -0.83 and -1.09 V (versus Ag/AgCl), respectively. The sensitivity of P2 was much higher than that of P1. The peak current of P2 was proportional to the concentration of bleomycin over the range 1.0 x 10(-9)-1.0 x 10(-7) M with a detection limit of 5.0 x 10(-10) M using adsorptive voltammetry at an accumulation time of 120 s (Ei = -0.80 V). The behaviour of the reduction wave was studied and applied to the determination of bleomycin in mouse serum. The reduction process of P1 was irreversible with adsorptive characteristics and the adsorption behavior obeyed the Frumkin adsorptive isotherm. The adsorptive coefficient beta was 8.9 x 10(5), the interaction factor alpha was 0.94 and the Gibbs energy of adsorption delta G degree was -33.93 kJ mol-1. P2 was an irreversible adsorption peak with catalytic hydrogen properties.
Tan
X
X
Department Of Chemistry, Beijing Normal University, China.
Hu
J
J
Li
Q
Q
D000327:Adsorption; D000964:Antimetabolites, Antineoplastic / Q000032:analysis* / Q000737:chemistry; D001761:Bleomycin / Q000032:analysis* / Q000737:chemistry; D004563:Electrochemistry
D000964:Antimetabolites, Antineoplastic; D001761:Bleomycin