Increased expression of peripheral benzodiazepine receptors in the facial nucleus following motor neuron axotomy.
Peripheral benzodiazepine receptors (PBRs) are expressed in a variety of tissues but are normally found at low levels in the brain. Following various types of nerve injury, a reactive gliosis results that exhibits a high expression of this receptor. To further characterize the expression of PBRs following neuronal injury, we evaluated PBR expression in the facial nucleus following facial nerve axotomy (FNA). Injury to a peripheral nerve results in a complex series of metabolic and morphological changes around the injured neuron. Transections of the facial nerve results in a rapid activation of both astrocytes and microglia around axotomized motor neurons. FNA resulted in an increase in the staining for both astrocytes (glial fibrillary acidic protein) and activated microglia (OX42). There was also a reduction in synaptic contacts with the motor nucleus as evidenced by reduced staining for the synaptic marker, synaptophysin. In sections labeled with [3H]-PK11195, the subsequent autoradiograms displayed marked increases in the labeling for PBRs. This increase was observed at 5, 7 and 10 days after nerve transection. The increase was primarily in the level of expression (Bmax), with no change in the affinity of the ligand (Kd). The increase in PBR expression after FNA supports the hypothesis that PBRs can be used as a sensitive marker for CNS injury.
Gehlert
D R
DR
Eli Lilly and Co., CNS Division, Indianapolis, IN 46285, USA. Gehlert-Donald-R@Lilly.com
Stephenson
D T
DT
Schober
D A
DA
Rash
K
K
Clemens
J A
JA
D000818:Animals; D001345:Autoradiography; D019771:Axotomy; D001921:Brain / Q000378:metabolism* / Q000503:physiopathology; D001923:Brain Chemistry; D005154:Facial Nerve / Q000378:metabolism* / Q000502:physiology*; D007546:Isoquinolines / Q000378:metabolism; D008024:Ligands; D008297:Male; D008959:Models, Neurological; D009046:Motor Neurons / Q000502:physiology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D011963:Receptors, GABA-A / Q000096:biosynthesis* / Q000502:physiology
D007546:Isoquinolines; D008024:Ligands; D011963:Receptors, GABA-A; C037850:PK 11195