Glutathione S-transferases in human ovarian follicular fluid.
OBJECTIVE
To study the levels of glutathione S-transferase Alpha 1-1 and glutathione S-transferase Pi 1-1 in human preovulatory ovarian follicular fluid (FF) and pooled granulosa and cumulus cells.


METHODS
The relation of glutathione S-transferase Alpha 1-1 and glutathione S-transferase Pi 1-1 with P and 17 beta-E2 concentrations were studied.


METHODS
The Department of Obstetrics and Gynecology, the Department of Gastroenterology, and the Laboratory of Endocrinology and Reproduction of the University Hospital Nijmegen in Nijmegen, the Netherlands.


METHODS
Infertile women participating in an IVF program.


RESULTS
Detectable amounts of glutathione S-transferase Alpha 1-1 and glutathione S-transferase Pi 1-1 were found in ovarian FF and pooled cumulus and granulosa cells. Concentrations of glutathione S-transferase Alpha 1-1 were always much higher than those of glutathione S-transferase Pi 1-1. Both ovarian FF concentrations of glutathione S-transferase Alpha 1-1 and glutathione S-transferase Pi 1-1 did not correlate with ovarian FF concentrations of 17 beta-E2 and P.


CONCLUSIONS
The high FF concentrations of glutathione S-transferase Pi 1-1 and especially of glutathione S-transferase Alpha 1-1 suggest that these enzymes may play an important role in the detoxification processes in the follicles. The lack of correlation between follicular P and 17 beta-E2 and glutathione S-transferase Alpha 1-1 and glutathione S-transferase Pi 1-1 indicates that both enzymes presumably are not present as a result of the high steroid levels.
Bisseling
J G
JG
Department of Obstetrics and Gynecology, University Hospital St. Radboud, Nijmegen, The Netherlands.
Knapen
M F
MF
Goverde
H J
HJ
Mulder
T P
TP
Peters
W H
WH
Willemsen
W N
WN
Thomas
C M
CM
Steegers
E A
EA
D004958:Estradiol / Q000032:analysis; D005260:Female; D015571:Follicular Fluid / Q000201:enzymology*; D005982:Glutathione Transferase / Q000032:analysis*; D006107:Granulosa Cells / Q000201:enzymology; D006801:Humans; D007527:Isoenzymes / Q000032:analysis*; D010053:Ovary / Q000201:enzymology*; D011374:Progesterone / Q000032:analysis; D012016:Reference Values
D007527:Isoenzymes; D011374:Progesterone; D004958:Estradiol; D005982:Glutathione Transferase