Cloning and characterization of a putative human RNA helicase gene of the DEAH-box protein family.
A human RNA helicase gene, DBP1, was cloned by PCR methodsusing degenerate oligonucleotide primers corresponding to highly conserved motifs among known members of the DEAH-box protein family. The full-length DBP1 contains 3028 nucleotides and codes for a protein of 813 amino acids with a calculated mol. wt. of 92723 daltons. The predicted amino acid sequence shares extensive homology with Prp2, Prp16, and Prp22 proteins, which are required to splice mRNA precursors in budding yeast. The protein encoded by DBP1 has RGD, RD, and HS(A/T) repeat motifs close to the N-terminus. Southern blot analysis suggested the presence of a homologue of the DBP1 genes in other species, and Northern blot analysis showed that DBP1 is expressed ubiquitously in various human organs investigated. The DBP1 gene was found to be on chromosome 4p15.3 and encodes a putative nuclear ATP-dependent RNA helicase.
Imamura
O
O
AGENE Research Institute, Kamakura, Japan.
Sugawara
M
M
Furuichi
Y
Y
D000251:Adenosine Triphosphatases / Q000737:chemistry; D017398:Alternative Splicing; D000595:Amino Acid Sequence; D001483:Base Sequence; D002874:Chromosome Mapping; D002894:Chromosomes, Human, Pair 4; D003001:Cloning, Molecular; D053487:DEAD-box RNA Helicases; D005656:Fungal Proteins / Q000737:chemistry; D006801:Humans; D008969:Molecular Sequence Data; D020365:RNA Helicases; D012316:RNA Nucleotidyltransferases / Q000096:biosynthesis* / Q000737:chemistry / Q000235:genetics*; D012322:RNA Precursors / Q000378:metabolism; D012326:RNA Splicing; D000072260:RNA Splicing Factors; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000737:chemistry; D029701:Saccharomyces cerevisiae Proteins; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid; D016335:Zinc Fingers
D005656:Fungal Proteins; D012322:RNA Precursors; D000072260:RNA Splicing Factors; D011994:Recombinant Proteins; D029701:Saccharomyces cerevisiae Proteins; D012316:RNA Nucleotidyltransferases; D000251:Adenosine Triphosphatases; C075669:PRP16 protein, S cerevisiae; C066490:PRP2 protein, S cerevisiae; C067135:PRP22 protein, S cerevisiae; D053487:DEAD-box RNA Helicases; D020365:RNA Helicases