Potency testing of inactivated tissue culture vaccine against Japanese encephalitis using antigen capture ELISA.
The potency of vaccines against Japanese encephalitis (JE) is usually determined by a mouse challenge test. In the present study, an antigen capture enzyme-linked immunosorbent assay (ELISA) using monoclonal antibodies (MoAbs) was used to screen inactivated tissue culture JE vaccine lots. This test is simple, quick and reliable besides being very sensitive and specific.
Joshi
A M
AM
National Institute of Virology, Pune, India.
Walimbe
A M
AM
Banerjee
K
K
D000818:Animals; D000911:Antibodies, Monoclonal; D046508:Culture Techniques; D004664:Encephalitis Virus, Japanese / Q000276:immunology*; D004672:Encephalitis, Japanese / Q000276:immunology / Q000503:physiopathology / Q000517:prevention & control*; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D051379:Mice; D012044:Regression Analysis; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D015164:Vaccines, Inactivated; D014765:Viral Vaccines
D000911:Antibodies, Monoclonal; D015164:Vaccines, Inactivated; D014765:Viral Vaccines