[Inhibition of experimental metastasis and cell adhesion of B16 murine melanoma cells by protein kinase C inhibitor CJ9111].
Treatment of B16 murine melanoma cells with different concentration of CJ9111 for various time in culture and subsequent i.v. injection of the cells into C57BL/6 mice resulted in dose and time dependent inhibition of lung metastasis. The 50% inhibition concentration (IC50) of metastasis with CJ9111 was 8 micrograms/ml, a value similar to that for the inhibition of B16 cell membrane-associated PKC (IC50 = 63 micrograms/ml). B16 cells treated with CJ9111 for 24 h were less adherent than untreated cells to endothelial cells. These results suggest that PKC may be a key element in regulating tumor-cell metastasis and PKC inhibitors may be promising agents in the control of metastasis.
Peng
T
T
Institute of Medicinal Biotechnology, Chinese Academy of Medical Sciences, Peking Union Medical College, Beijing.
Yao
C
C
Xue
L
L
D000818:Animals; D002417:Cattle; D002448:Cell Adhesion / Q000187:drug effects; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D004730:Endothelium, Vascular / Q000166:cytology; D005260:Female; D008175:Lung Neoplasms / Q000517:prevention & control* / Q000556:secondary; D008546:Melanoma, Experimental / Q000473:pathology*; D051379:Mice; D008810:Mice, Inbred C57BL; D009368:Neoplasm Transplantation; D011493:Protein Kinase C / Q000037:antagonists & inhibitors*; D014407:Tumor Cells, Cultured / Q000187:drug effects
D004365:Drugs, Chinese Herbal; D011493:Protein Kinase C