[Invasion of fibrosarcoma cells transfected with PAI-2 gene].
The effect of PAI-2 on the invasion of fibrosarcoma cells in vitro and in vivo was investigated. The control cells (C+, C+ pem) and PAI-2 transfectants (C+ exp) were used in the assay of the degradation of isotopically labelld 3H-ECM and were injected sc into athymic/nude mice. Recombinant PAI-2 could inhibit efficiently the degradation of 3H-ECM by tumor cells (86.5%). The PAI-2 transfectants remained tumorigenic in nude mice, but tumors originating from the PAI-2 transfectants showed histologically the presence of a thick capsule which was absent in tumors from control cells.
Cao
X
X
Department of Biology, Nanjing Normal University.
Zhang
L
L
Su
C
C
D000818:Animals; D005354:Fibrosarcoma / Q000473:pathology*; D006801:Humans; D051379:Mice; D008819:Mice, Nude; D009361:Neoplasm Invasiveness; D009368:Neoplasm Transplantation; D017396:Plasminogen Activator Inhibitor 2 / Q000235:genetics*; D014162:Transfection; D014407:Tumor Cells, Cultured
D017396:Plasminogen Activator Inhibitor 2