[Inhibitory effects of gypenoside on rat heart and brain Na+, K+(-)ATPase activity].
The effects of gypenoside (Gyp) on the microsomal Na+, K(+)-ATPase from rat hearts and brains were investigated in comparison with ginsenoside (Gin). The results showed that Gyp could inhibit the enzyme activity rapidly and reversibly in vitro. The inhibition was in a concentration-depend manner. The IC50 of Gyp for the heart and brain was 58.79 +/- 8.05 mg/L and 52.07 +/- 6.25 mg/L, respectively. Kinetic analysis revealed that Gyp was an incompetitive inhibitor of ATP. These results suggest that the positive inotrope effect and the inhibition of CNS by Gyp are related to the in hibitory action of Gyp on the actiyily of Na+, K(+)-ATPase from hearts or brains.
Han
X
X
Luoyang Medical Institute.
Wei
H
H
Wang
Y
Y
Luo
C
C
D000818:Animals; D001921:Brain / Q000201:enzymology*; D002316:Cardiotonic Agents / Q000494:pharmacology*; D002492:Central Nervous System Depressants / Q000494:pharmacology*; D008297:Male; D008861:Microsomes / Q000201:enzymology; D009206:Myocardium / Q000201:enzymology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D012503:Saponins / Q000494:pharmacology*; D000254:Sodium-Potassium-Exchanging ATPase / Q000378:metabolism*; D013268:Stimulation, Chemical
D002316:Cardiotonic Agents; D002492:Central Nervous System Depressants; D012503:Saponins; C077955:gypenoside IX; D000254:Sodium-Potassium-Exchanging ATPase