Protein phi and psi dihedral restraints determined from multidimensional hypersurface correlations of backbone chemical shifts and their use in the determination of protein tertiary structures.
The chemical shifts of the backbone atoms of proteins can be used to obtain restraints that can be incorporated into structure determination methods. Each chemical shift can be used to define a restraint and these restraints can be simultaneously used to define the local, secondary structure features. The global fold can be determined by a combined use of the chemical shift based restraints along with the long-range information present in the NOEs of partially deuterated proteins or the amide-amide NOEs but not from such limited NOE data sets alone. This approach has been demonstrated to be capable of determining the overall folding pattern of four proteins. This suggests that solution-state NMR methods can be extended to the structure determination of larger proteins by using the information present in the chemical shifts of the backbone atoms along with the data that can be obtained on a small number of labeled forms.
Beger
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RD
Department of Chemistry, Wesleyan University, Middletown, CT 06459, USA.
Bolton
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PH
D000596:Amino Acids / Q000737:chemistry; D000900:Anti-Bacterial Agents / Q000737:chemistry; D002247:Carbon Isotopes; D002352:Carrier Proteins / Q000737:chemistry; D003285:Contractile Proteins; D016208:Databases, Factual; D036341:Intercellular Signaling Peptides and Proteins; D008263:Macrophage Migration-Inhibitory Factors / Q000737:chemistry; D008840:Microfilament Proteins / Q000737:chemistry; D008958:Models, Molecular; D009587:Nitrogen Isotopes; D019906:Nuclear Magnetic Resonance, Biomolecular / Q000379:methods*; D010455:Peptides; D051304:Profilins; D017433:Protein Structure, Secondary; D017434:Protein Structure, Tertiary
D000596:Amino Acids; D000900:Anti-Bacterial Agents; D002247:Carbon Isotopes; D002352:Carrier Proteins; D003285:Contractile Proteins; D036341:Intercellular Signaling Peptides and Proteins; D008263:Macrophage Migration-Inhibitory Factors; D008840:Microfilament Proteins; D009587:Nitrogen Isotopes; D010455:Peptides; D051304:Profilins; C026615:villin; C069636:kedarcidin peptide