A guinea-pig model of ultrasonically nebulized distilled water-induced bronchoconstriction.
Ultrasonically nebulized distilled water-induced bronchoconstriction (UNDW-IB) is specific to asthma. The mechanisms underlying UNDW-IB are not fully understood, and no reproducible animal model has been reported. The purpose of this study was to develop a guinea-pig model of UNDW-IB. Ultrasonically nebulized distilled water (UNDW) was inhaled 20 min after an aerosolized antigen challenge in passively sensitized and artificially ventilated guinea-pigs. UNDW was also inhaled 5 and 20 min after 0.1 mg x mL(-1) methacholine inhalation in nonsensitized animals. In addition, 0.1 mg x kg(-1) S-1452, a thromboxane A2 antagonist, or saline was given intravenously 5 min before UNDW inhalation in sensitized animals. The inhalation of UNDW caused bronchoconstriction, when inhaled 20 min after an antigen challenge in sensitized guinea-pigs. UNDW inhalation did not produce bronchoconstriction after saline inhalation in nonsensitized or sensitized guinea-pigs, or after antigen inhalation in nonsensitized animals. Methacholine-induced bronchoconstriction did not evoke UNDW-IB. Neither did S-1452 reduce the UNDW-IB. In conclusion, the guinea-pig model of ultrasonically nebulized distilled water-induced bronchoconstriction developed in this study suggests that allergic reaction, but not bronchoconstriction, can induce bronchial hyperresponsiveness to ultrasonically nebulized distilled water, and that thromboxane A2 is not involved in ultrasonically nebulized distilled water-induced bronchoconstriction.
Fujimura
M
M
The Third Dept of Internal Medicine, Kanazawa University School of Medicine, Japan.
Amemiya
M
M
Myou
S
S
Mizuguchi
M
M
Matsuda
T
T
D000280:Administration, Inhalation; D000420:Albuterol / Q000008:administration & dosage; D000818:Animals; D001249:Asthma / Q000175:diagnosis / Q000503:physiopathology*; D016535:Bronchial Hyperreactivity / Q000503:physiopathology*; D001985:Bronchial Provocation Tests / Q000379:methods*; D016084:Bronchoconstriction / Q000187:drug effects*; D001993:Bronchodilator Agents / Q000008:administration & dosage; D004195:Disease Models, Animal; D006168:Guinea Pigs; D008297:Male; D009330:Nebulizers and Vaporizers; D012016:Reference Values; D013928:Thromboxane A2 / Q000008:administration & dosage / Q000502:physiology; D014867:Water / Q000008:administration & dosage*
D001993:Bronchodilator Agents; D014867:Water; D013928:Thromboxane A2; D000420:Albuterol