Diagnostic value of p53 protein and flow cytometric DNA analysis in the study of serous effusions.
OBJECTIVE
To investigate the diagnostic value of p53 protein and DNA analysis in the study of serous effusions.


METHODS
A total of 76 samples of serous effusions were studied by immunohistochemistry for p53 protein and flow cytometric (FCM) DNA analysis. The results were correlated with final cytologic diagnoses, which were confirmed by immunohistochemistry using antibodies against cytokeratin, carcinoembryonic antigen, epithelial membrane antigen and fibronectin.


RESULTS
Final cytologic diagnoses included 28 malignant effusions and 48 benign effusions. No expression of p53 protein was seen in benign effusions. In contrast, p53 protein expression was seen in 19/28 (sensitivity 68%) malignant effusions. FCM detected aneuploid cells in 12/28 (43% sensitivity) of malignant and 0/46 of benign effusions. Immunohistochemical determination of p53 protein combined with FCM DNA analysis increased sensitivity to 79%.


CONCLUSIONS
Immunohistochemical determination of p53 protein and FCM DNA analysis can aid in making an accurate and specific diagnosis of serous effusions, but the principal limitation of these tests is their relatively low sensitivity.
Lee
J S
JS
Department of Pathology, Chonnam University Medical School, Kwangju, Seoul, Korea.
Lee
M C
MC
Park
C S
CS
Juhng
S W
SW
D000782:Aneuploidy; D014408:Biomarkers, Tumor / Q000032:analysis*; D002272:Carcinoembryonic Antigen / Q000032:analysis; D004247:DNA / Q000032:analysis*; D004273:DNA, Neoplasm / Q000032:analysis*; D005122:Exudates and Transudates / Q000737:chemistry* / Q000166:cytology*; D005353:Fibronectins / Q000032:analysis; D005434:Flow Cytometry / Q000379:methods; D006801:Humans; D007150:Immunohistochemistry / Q000379:methods; D007633:Keratins / Q000032:analysis; D018396:Mucin-1 / Q000032:analysis; D009369:Neoplasms / Q000473:pathology*; D011237:Predictive Value of Tests; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D016159:Tumor Suppressor Protein p53 / Q000032:analysis*
D014408:Biomarkers, Tumor; D002272:Carcinoembryonic Antigen; D004273:DNA, Neoplasm; D005353:Fibronectins; D018396:Mucin-1; D016159:Tumor Suppressor Protein p53; D007633:Keratins; D004247:DNA