Acute methionine load-induced hyperhomocysteinemia enhances platelet aggregation, thromboxane biosynthesis, and macrophage-derived tissue factor activity in rats.
A moderate elevation of plasma homocysteine is a risk factor for atherosclerosis and arterial and veinous thrombosis. However, the mechanisms leading to vascular disorders are poorly understood because studies that have investigated the potential atherothrombogenicity of hyperhomocysteinemia in vivo are scarce. Using a rat model, we were the first to show that dietary folic acid deficiency, a major cause of basal hyperhomocysteinemia, is associated with enhanced macrophage-derived tissue factor and platelet activities. We proposed that an homocysteine-induced oxidative stress may account for this hypercoagulable state. To determine the true thrombogenicity of moderate hyperhomocysteinemia and better understand its etiology, we have carried out an acute methionine load in control and folate-deficient animals. When rats were fed the control diet, a transient fourfold increase in plasma homocysteine levels was observed 2 h after the methionine administration. As with prolonged dietary folic acid deficiency, this methionine load potentiated the platelet aggregation in response to thrombin and ADP as well as the thrombin-induced thromboxane synthesis. It also stimulated the basal and lipopolysaccharide-induced tissue factor activity of peritoneal macrophages. These prothrombotic effects were associated with an increased lipid peroxidation characterized by an elevation of plasma conjugated dienes, lipid hydroperoxides, and thiobarbituric acid-reactive substances. When rats were fed a folic acid-deficient diet, the methionine load did not cause any further increase in plasma homocysteine concentration, platelet activation, macrophage tissue factor-dependent coagulation, or lipoperoxidation. Altogether, our data showed that the prethrombotic state due to both the altered remethylation and transsulfuration pathways resulted from the moderate elevation of circulating homocysteine. We conclude that moderate hyperhomocysteinemia plays a role in the development of a thrombogenic state that might be mediated by the occurrence of oxidative stress.
Durand
P
P
INSERM CJF 93-10, Laboratoire de Biochimie des Lipoprotéines, Faculté de Médecine, Université de Bourgogne, Dijon, France.
Lussier-Cacan
S
S
Blache
D
D
D000244:Adenosine Diphosphate / Q000494:pharmacology; D000596:Amino Acids / Q000097:blood; D000704:Analysis of Variance; D000818:Animals; D001792:Blood Platelets / Q000187:drug effects / Q000502:physiology*; D004032:Diet; D005494:Folic Acid Deficiency / Q000097:blood / Q000503:physiopathology*; D005978:Glutathione / Q000097:blood; D006710:Homocysteine / Q000097:blood*; D015227:Lipid Peroxidation; D017737:Macrophages, Peritoneal / Q000187:drug effects / Q000378:metabolism*; D008297:Male; D008315:Malondialdehyde / Q000032:analysis; D008715:Methionine / Q000008:administration & dosage / Q000494:pharmacology*; D010974:Platelet Aggregation / Q000187:drug effects / Q000502:physiology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D012044:Regression Analysis; D017392:Thiobarbituric Acid Reactive Substances / Q000032:analysis; D013917:Thrombin / Q000494:pharmacology; D013925:Thromboplastin / Q000096:biosynthesis*; D013931:Thromboxanes / Q000096:biosynthesis / Q000097:blood*
D000596:Amino Acids; D017392:Thiobarbituric Acid Reactive Substances; D013931:Thromboxanes; D006710:Homocysteine; D008315:Malondialdehyde; D000244:Adenosine Diphosphate; D013925:Thromboplastin; D008715:Methionine; D013917:Thrombin; D005978:Glutathione