Fibroblast growth factor-2 expression is up-regulated after denervation in rat lung tissue.
To understand the role of fibroblast growth factor-2 (FGF-2) during the denervation-reinnervation processes which occur after lung transplantation, we studied FGF-2 gene expression in a rat lung denervation model. The temporal profile of FGF-2 mRNA in denervated rat lungs was quantitatively assessed by competitive reverse transcription polymerase chain reaction (RT-PCR) method. The level of FGF-2 mRNA was consistently higher in denervated lungs, showing a peak value on the 5th post-operative day. Immunohistochemical analysis with an anti-FGF-2 monoclonal antibody disclosed immunoreactivity in Schwann cells at the distal severed end of the nerve fascicle located at the lung hilus, 1 week post-surgery. This study indicates that FGF-2 gene expression is up-regulated following denervation and suggests possible roles of FGF-2 in the reinnervation process of lung tissue.
Shimada
J
J
Second Department of Surgery, Kyoto Prefectural University of Medicine, Japan.
Fushiki
S
S
Tsujimura
A
A
Oka
T
T
D000818:Animals; D000911:Antibodies, Monoclonal; D003714:Denervation; D016222:Fibroblast Growth Factor 2 / Q000032:analysis / Q000096:biosynthesis*; D005786:Gene Expression Regulation; D007150:Immunohistochemistry; D008168:Lung / Q000166:cytology / Q000294:innervation* / Q000378:metabolism*; D008297:Male; D009416:Nerve Regeneration; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000096:biosynthesis; D051381:Rats; D011917:Rats, Inbred Lew; D012583:Schwann Cells / Q000166:cytology / Q000378:metabolism*; D014158:Transcription, Genetic; D014630:Vagus Nerve / Q000166:cytology / Q000502:physiology*
D000911:Antibodies, Monoclonal; D012333:RNA, Messenger; D016222:Fibroblast Growth Factor 2