title: Abnormal DNA synthesis activity induced by X-rays in nevoid basal cell carcinoma syndrome cells.
abstract: DNA synthesis activity was examined in fibroblasts and isolated nuclei derived from patients with nevoid basal cell carcinoma syndrome (NBCCS) upon exposure to X-ray and ultraviolet (UV). The DNA synthesis activity in NBCCS fibroblasts increased after X-ray irradiation, i.e., to twice that on mock-irradiation, while it decreased in healthy donor-derived fibroblasts. The DNA synthesis activity in isolated nuclei of X-ray irradiated NBCCS fibroblasts also increased, i.e., more than twice that on mock-irradiated. In the experiments using synchronized cells, DNA synthesis activity showed the most marked increase when the fibroblasts at S phase were irradiated with X-rays. In contrast, UV-irradiated NBCCS fibroblasts showed no such increase in DNA synthesis. These results revealed that DNA synthesis is abnormally induced in X-ray irradiated NBCCS cells and that this abnormality might be related with the tendency of tumorigenesis in NBCCS patients after exposure to X-ray.
authors.affiliation: Department of Pediatrics, School of Medicine, Chiba University, Japan. kfujii@nch.go.jp
authors.forename: K
authors.initials: K
authors.lastname: Fujii
authors.forename: N
authors.initials: N
authors.lastname: Suzuki
authors.forename: S
authors.initials: S
authors.lastname: Ishijima
authors.forename: K
authors.initials: K
authors.lastname: Kita
authors.forename: T
authors.initials: T
authors.lastname: Sonoda
authors.forename: M
authors.initials: M
authors.lastname: Dezawa
authors.forename: K
authors.initials: K
authors.lastname: Sugita
authors.forename: H
authors.initials: H
authors.lastname: Niimi
mesh_terms: D001260:Ataxia Telangiectasia; D001478:Basal Cell Nevus Syndrome / Q000378:metabolism* / Q000473:pathology; D002460:Cell Line; D002467:Cell Nucleus / Q000378:metabolism / Q000528:radiation effects; D004261:DNA Replication / Q000528:radiation effects*; D004273:DNA, Neoplasm / Q000096:biosynthesis* / Q000528:radiation effects; D005347:Fibroblasts; D006801:Humans; D007700:Kinetics; D008840:Microfilament Proteins; D014176:Protein Biosynthesis; D011506:Proteins / Q000737:chemistry; D012878:Skin Neoplasms / Q000378:metabolism* / Q000473:pathology; D013942:Thymine Nucleotides / Q000378:metabolism; D014407:Tumor Cells, Cultured; D014466:Ultraviolet Rays; D014965:X-Rays
chemical_list: D004273:DNA, Neoplasm; D008840:Microfilament Proteins; D011506:Proteins; C109333:Tex9 protein, mouse; D013942:Thymine Nucleotides; C024157:thymidine 5'-triphosphate