title: Disinfection of bronchoscopes, contaminated in vitro with Mycobacterium tuberculosis, Mycobacterium avium-intracellulare and Mycobacterium chelonae in sputum, using stabilized, buffered peracetic acid solution ('Nu-Cidex').
abstract: The efficacy of 0.35% stabilized buffered peracetic acid solution ('Nu-Cidex') against clinical isolates of Mycobacterium tuberculosis, Mycobacterium avium-intracellulare and Mycobacterium chelonae in homogenized sputum was tested. An in-use method, using an automated bronchoscope washing machine, showed that over 10 cycles, at a disinfectant contact time of 5 min, M. tuberculosis and M. chelonae were effectively eradicated from the bronchoscope, even in the absence of detergent and pre-cleaning. M. avium-intracellulare was not eradicated in only one of 10 cycles with contact times of 5 and 10 min, but numbers were reduced by > 7 log10 and > 5 log10, respectively. With detergent present, M. avium-intracellulare was successfully eradicated in all cycles at a contact time of 5 min or greater. The results demonstrate that peracetic acid is an effective mycobactericidal agent for use in the disinfection of bronchoscopes.
authors.affiliation: Department of Microbiology, Royal Brompton Hospital, London.
authors.forename: A M
authors.initials: AM
authors.lastname: Middleton
authors.forename: M V
authors.initials: MV
authors.lastname: Chadwick
authors.forename: H
authors.initials: H
authors.lastname: Gaya
mesh_terms: D020680:Bronchoscopes; D004202:Disinfectants / Q000494:pharmacology*; D004203:Disinfection / Q000379:methods*; D004866:Equipment Contamination; D006801:Humans; D015269:Mycobacterium avium Complex / Q000187:drug effects* / Q000302:isolation & purification; D016926:Mycobacterium chelonae / Q000187:drug effects* / Q000302:isolation & purification; D009169:Mycobacterium tuberculosis / Q000187:drug effects* / Q000302:isolation & purification; D010463:Peracetic Acid / Q000494:pharmacology*; D013183:Sputum / Q000382:microbiology
chemical_list: D004202:Disinfectants; D010463:Peracetic Acid