Fluorometric measurement of yessotoxins in shellfish by high-pressure liquid chromatography.
A rapid HPLC method with fluorescence detection of yessotoxin (YTX) and its two analogs (45-OHYTX and norYTX) in mussels and scallops is presented. A dienophile reagent, DMEQ-TAD, was used for fluorescence labeling. YTX was measured in the range 1-100 ng. The method confirmed the occurrence of YTX and 45-OHYTX for the first time in mussels from Chile and New Zealand.
Yasumoto
T
T
Faculty of Agriculture, Tohoku University, Sendai, Japan.
Takizawa
A
A
D000818:Animals; D049872:Bivalvia / Q000737:chemistry; D002851:Chromatography, High Pressure Liquid; D004064:Digestive System; D004988:Ethers, Cyclic / Q000032:analysis* / Q000737:chemistry; D005470:Fluorometry / Q000379:methods; D008978:Mollusk Venoms / Q000032:analysis*; D035964:Oxocins; D012758:Shellfish / Q000032:analysis*
D004988:Ethers, Cyclic; D008978:Mollusk Venoms; D035964:Oxocins; C066632:yessotoxin