title: Morphological evaluation of osteoblasts cultured on different calcium phosphate ceramics.
abstract: The objective of these investigations was to develop an in vitro test system for evaluating novel rapidly resorbable calcium phosphate ceramics of varying composition. Rat bone marrow cells were cultured on the disc-shaped test substrates for 14 days. Five calcium phosphates were examined: R1 CaNaPO4; R1/M2, composed of CaNaPO4 and MgNaPO4; R1/2, composed of CaNaPO4 and Mg2SiO4; R1 + 9% SiO2 consisting of CaNaPO4 and 9% SiO2 (wt%) and R17, Ca2KNa(PO4)2. Two studies were performed. In study I cultures were re-fed every two to three days. In study II the medium was changed daily, and calcium and phosphate concentrations of the medium were determined daily. Specimens were prepared for light microscopy and morphometric evaluation of the cell-covered substrate area, scanning electron microscopy and energy-dispersive X-ray analysis. With all materials tested except for R1/2, an increase of cellular growth was observed after changing the medium daily. Of the different calcium phosphate ceramics tested, R17 and R1/M2 facilitated osteoblast growth and elaboration of extracellular matrix to the highest degree. The inhibition of cell growth encountered with R1 in study I and R1/2 in both studies seemed to be related to a high phosphate-ion release from these materials.
authors.affiliation: Department of Restorative Dentistry and Periodontology, University Hospital Benjamin Franklin, Free University of Berlin, Germany.
authors.forename: C
authors.initials: C
authors.lastname: Knabe
authors.forename: R
authors.initials: R
authors.lastname: Gildenhaar
authors.forename: G
authors.initials: G
authors.lastname: Berger
authors.forename: W
authors.initials: W
authors.lastname: Ostapowicz
authors.forename: R
authors.initials: R
authors.lastname: Fitzner
authors.forename: R J
authors.initials: RJ
authors.lastname: Radlanski
authors.forename: U
authors.initials: U
authors.lastname: Gross
mesh_terms: D000042:Absorption; D000818:Animals; D001672:Biocompatible Materials / Q000378:metabolism / Q000493:pharmacokinetics* / Q000494:pharmacology; D001854:Bone Marrow Cells / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism; D002130:Calcium Phosphates / Q000378:metabolism / Q000493:pharmacokinetics* / Q000494:pharmacology; D002478:Cells, Cultured; D002516:Ceramics; D003470:Culture Media; D004353:Drug Evaluation, Preclinical / Q000379:methods; D004577:Electron Probe Microanalysis; D008855:Microscopy, Electron, Scanning; D010006:Osteoblasts / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar
chemical_list: D001672:Biocompatible Materials; D002130:Calcium Phosphates; D003470:Culture Media; C485828:alpha-tricalcium phosphate; C485830:tetracalcium phosphate; C485838:calcium phosphate, monobasic, anhydrous; C020243:calcium phosphate; C485829:calcium phosphate, dibasic, anhydrous