Inhibition of gene expression from the human c-erbB gene promoter by a retroviral vector expressing anti-gene RNA.
Anti-gene is a potent inhibitor of transcriptional promoter activity and subsequent gene expression. This property has been exploited to suppress the expression of a variety of oncogenes for regulating tumor proliferation or viral activities. In this paper, we describe a novel retroviral vector designed to express human c-erbB anti-gene RNA and to reduce the promoter activity in the cells. Mouse fibroblast NIH3T3 cells were stably transfected with an expression construct containing a truncated human c-erbB gene promoter fused to the firefly luciferase reporter gene. Infection into these cells of the c-erbB anti-gene retroviral vector targeted to the 26 bp pyrimidine-rich element in the human c-erbB gene promoter resulted in a dose-dependent decrease in the luciferase activity of the cells. Retroviral vector expressing anti-gene RNA may be useful as an alternative program of gene regulation in the cells.
Okada
T
T
Department of Neurosurgery, Kanazawa University School of Medicine, Japan. tokada@nhgri.nih.gov
Amanuma
H
H
Okada
Y
Y
Obata
M
M
Hayashi
Y
Y
Yamaguchi
K
K
Yamashita
J
J
D016475:3T3 Cells; D000818:Animals; D001483:Base Sequence; D003587:Cytomegalovirus / Q000235:genetics; D005786:Gene Expression Regulation / Q000187:drug effects; D017930:Genes, Reporter; D018815:Genes, erbB / Q000187:drug effects; D005822:Genetic Vectors / Q000378:metabolism* / Q000494:pharmacology; D006801:Humans; D008156:Luciferases / Q000235:genetics; D051379:Mice; D008969:Molecular Sequence Data; D011401:Promoter Regions, Genetic / Q000187:drug effects; D012313:RNA / Q000096:biosynthesis; D012190:Retroviridae / Q000235:genetics*; D015719:Single-Strand Specific DNA and RNA Endonucleases / Q000235:genetics
D012313:RNA; D008156:Luciferases; D015719:Single-Strand Specific DNA and RNA Endonucleases