[Ultramicromethod of determination of fructose in the semen].
A sensitive, accurate, and available method is proposed for fructose measurement in the semen. It is based on a color reaction of thiobarbituric acid and fructose (hydroxymethylfurfural). The range of the method is 1.4 to 38.9 mumoles/ml (0.25-7.0 mg/ml)(the normal range is 6.7 to 33.3 mumoles/ml (1.2-6.0 mg/ml) and the study can be performed with just 0.01 ml of the semen. A common water bath is used instead of a thermostat. The measurements are carried out using an available KPK-3 or PEK-56M spectrophotometer with a mercury lamp and filter No. 4. The mercury lamp is much more intensive than a common incandescent lamp, this permitting the use photoelements which lost their original sensitivity. A flaw of the method is use of concentrated hydrochloric acid.
Pashkov
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D003124:Colorimetry / Q000379:methods; D005632:Fructose / Q000032:analysis*; D005662:Furaldehyde / Q000031:analogs & derivatives; D006801:Humans; D007202:Indicators and Reagents; D008297:Male; D012661:Semen / Q000737:chemistry*; D013053:Spectrophotometry
D007202:Indicators and Reagents; D005632:Fructose; C008046:5-hydroxymethylfurfural; D005662:Furaldehyde