Molecular characterization of the human interleukin (IL)-17 receptor.
Human interleukin 17 (hIL-17) is a T-cell derived cytokine that exhibits 63% amino acid sequence identity to mouse IL-17 (mIL-17) and 57% identity to a viral protein encoded by the herpesvirus saimiri (HSV) gene 13 (HVS13). The IL-17 family of proteins binds to a unique mouse receptor (mIL-17R). Using nucleic acid hybridization techniques, a cDNA encoding a human homologue of the mIL-17R (hIL-17R) was isolated from a human T cell library. The predicted amino acid sequence of the hIL-17R is 69% identical to the mIL-17R, shares no homology with previously identified cytokine receptor families, and exhibits a broad tissue distribution. The hIL-17R gene was localized to chromosome 22. Monoclonal antibodies (mAbs) generated against the hIL-17R were able to block the IL-17-induced production of cytokine from human foreskin fibroblast (HFF) cells. Binding studies suggest that recombinant hIL-17 binds to the hIL-17R with low affinity.
Yao
Z
Z
Department of Molecular Biology, Immunex Corporation, Seattle, WA 98101, USA.
Spriggs
M K
MK
Derry
J M
JM
Strockbine
L
L
Park
L S
LS
VandenBos
T
T
Zappone
J D
JD
Painter
S L
SL
Armitage
R J
RJ
D000595:Amino Acid Sequence; D000818:Animals; D000911:Antibodies, Monoclonal; D000945:Antigens, Differentiation, T-Lymphocyte / Q000737:chemistry; D002469:Cell Separation; D002874:Chromosome Mapping; D002892:Chromosomes, Human, Pair 22; D004591:Electrophoresis, Polyacrylamide Gel; D005434:Flow Cytometry; D006801:Humans; D020381:Interleukin-17; D015850:Interleukin-6 / Q000096:biosynthesis; D007378:Interleukins / Q000037:antagonists & inhibitors / Q000378:metabolism*; D051379:Mice; D008969:Molecular Sequence Data; D008970:Molecular Weight; D012333:RNA, Messenger / Q000378:metabolism; D018123:Receptors, Interleukin / Q000235:genetics* / Q000276:immunology / Q000378:metabolism; D053722:Receptors, Interleukin-17; D011994:Recombinant Proteins / Q000235:genetics / Q000276:immunology / Q000378:metabolism; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid; D013601:T-Lymphocytes / Q000737:chemistry; D014018:Tissue Distribution
D000911:Antibodies, Monoclonal; D000945:Antigens, Differentiation, T-Lymphocyte; C109985:IL17RA protein, human; C098680:Il17a protein, mouse; C491673:Il17ra protein, mouse; D020381:Interleukin-17; D015850:Interleukin-6; D007378:Interleukins; D012333:RNA, Messenger; D018123:Receptors, Interleukin; D053722:Receptors, Interleukin-17; D011994:Recombinant Proteins