An immunocytochemical and ultrastructural study of the larval anterior intestine of the frog Rana temporaria, with especial reference to endocrine cells.
Endocrine cells of the larval intestine of Rana temporaria tadpoles have been identified by argyrophilic, immunocytochemical and electron-microscopical techniques. Scarce endocrine cells have been found in both the short non-absorptive zone immediately following the stomach, and in the rest of the anterior intestine. Endocrine cells are frequently seen to extend a cytoplasmic process towards the lumen. Immunoreactivity for serotonin, somatostatin, bombesin and cholecystokinin-8 has been detected. According to the ultrastructural traits of the endocrine granules, three larval intestinal endocrine populations have been differentiated.
Bodegas
M E
ME
Departamento de Histología y Anatomía Patológica, Facultad de Ciencias, Universidad de Navarra, Pamplona, Spain. Mbodegas@mail2.cti.unav.es
Villaro
A C
AC
Burrell
M A
MA
Rovira
J
J
Valverde
E
E
Ortiz De Zárate
A
A
Sesma
P
P
D000818:Animals; D002454:Cell Differentiation / Q000502:physiology; D019858:Enteroendocrine Cells / Q000737:chemistry* / Q000648:ultrastructure; D007150:Immunohistochemistry; D007422:Intestines / Q000737:chemistry* / Q000166:cytology; D007814:Larva / Q000737:chemistry / Q000648:ultrastructure; D011896:Rana temporaria