title: 6-N-hydroxylaminopurine (HAP)-induced accumulation of variability in haploid and diploid strains of Aspergillus nidulans.
abstract: Haploid and diploid strains of Aspergillus nidulans have been repeatedly treated with the strong mutagen 6-N-hydroxylaminopurine (HAP) which causes only base substitutions. An enormous amount of variability may be rapidly accumulated in haploid or diploid strains of A. nidulans. In particular, in the diploids the analysis of the results shows that after 12 cycles of treatment the conidia differ from each other for about ten recessive lethals and therefore probably for several hundreds of mutations. The viability of the heterozygous multiply mutant diploids is not appreciably different from that of untreated controls. In the diploid strains the accumulated variability was very high. The treatment of a haploid strain during vegetative growth also caused a strong accumulation of mutations, even though deleterious, because they can be maintained in the heterokaryotic condition.
authors.affiliation: Istituto di Anatomixa comparata, University of Perugia, Via Pascoli, I-06100 Perugia, Italy.
authors.forename: S
authors.initials: S
authors.lastname: Pimpinelli
authors.forename: A
authors.initials: A
authors.lastname: Marini
authors.forename: N
authors.initials: N
authors.lastname: Babudri
authors.forename: G
authors.initials: G
authors.lastname: Morpurgo
mesh_terms: D000225:Adenine / Q000031:analogs & derivatives* / Q000494:pharmacology; D001233:Aspergillus nidulans / Q000187:drug effects* / Q000235:genetics* / Q000254:growth & development; D004171:Diploidy; D005800:Genes, Fungal / Q000187:drug effects; D005804:Genes, Lethal / Q000187:drug effects; D005808:Genes, Recessive / Q000187:drug effects; D014644:Genetic Variation / Q000187:drug effects; D006238:Haploidy; D009153:Mutagens / Q000494:pharmacology*; D009154:Mutation
chemical_list: D009153:Mutagens; C032610:6-N-hydroxylaminopurine; D000225:Adenine