A note on the physiological background of the ethylene oxide adduct 7-(2-hydroxyethyl)guanine in DNA from human blood.
A newly developed high-performance liquid chromatography (HPLC) method involves derivatization with phenylglyoxal and fluorescence detection, using 7-methylguanine as internal standard. The physiological background of the adduct 7-(2-hydroxyethyl)guanine in DNA isolated from human blood was determined by this method. In five persons the range of the adduct was between 2.1 and 5.8 pmol/mg DNA (mean 3.2). This finding is consistent with previous data obtained by others, using different analytical methods, and points to an intrinsic carcinogenic risk due to endogenous ethylene oxide.
Bolt
H M
HM
Leutbecher
M
M
Golka
K
K
D002851:Chromatography, High Pressure Liquid; D018736:DNA Adducts / Q000097:blood*; D005027:Ethylene Oxide / Q000097:blood*; D006147:Guanine / Q000031:analogs & derivatives* / Q000097:blood; D006801:Humans; D013050:Spectrometry, Fluorescence; D013056:Spectrophotometry, Ultraviolet
D018736:DNA Adducts; C035018:N(7)-hydroxyethylguanine; D006147:Guanine; D005027:Ethylene Oxide