Mutagenicity of kaempferol in V79 cells: the role of cytochromes P450.
The flavonol kaempferol is widely found in the diet and is directly mutagenic in some short-term tests, such as the induction of chromosomal aberrations in eukaryotic cells. The presence of exogenous metabolizing systems enhances its mutagenicity. We have evaluated the role of cytochromes P450 in the induction of chromosomal aberrations by kaempferol in V79 cells. The results obtained suggest that there is a time-dependent biotransformation of kaempferol to quercetin, by cytochromes P450, as assessed by high pressure liquid chromatography. Quercetin seems to contribute to the mutagenicity of kaempferol in the presence of microsomal metabolizing systems. On the other hand, the direct induction of chromosomal aberrations by kaempferol does not seem to depend on the production of reactive oxygen species.
Silva
I D
ID
Department of Genetics, Faculty of Medical Sciences, New University of Lisbon, Portugal.
Rodrigues
A
A
Gaspar
J
J
Maia
R
R
Laires
A
A
Rueff
J
J
D000818:Animals; D001711:Biotransformation; D002374:Catalase / Q000378:metabolism; D002460:Cell Line; D002851:Chromatography, High Pressure Liquid; D002869:Chromosome Aberrations; D006224:Cricetinae; D003577:Cytochrome P-450 Enzyme System / Q000378:metabolism*; D005419:Flavonoids; D006863:Hydrogen-Ion Concentration; D044949:Kaempferols; D008297:Male; D015162:Micronucleus Tests; D008862:Microsomes, Liver / Q000378:metabolism; D011794:Quercetin / Q000031:analogs & derivatives* / Q000378:metabolism / Q000633:toxicity; D051381:Rats; D017382:Reactive Oxygen Species; D013482:Superoxide Dismutase / Q000378:metabolism
D005419:Flavonoids; D044949:Kaempferols; D017382:Reactive Oxygen Species; C006552:kaempferol; D003577:Cytochrome P-450 Enzyme System; D011794:Quercetin; D002374:Catalase; D013482:Superoxide Dismutase