title: The effect of a shear force on the cell shedding rate of the corneal epithelium.
abstract: OBJECTIVE
  During blinking the lids apply a shear force to the corneal epithelium. The aim of this study was to determine if a shear force applied to the epithelial surface increases the rate at which cells shed.
  
  
  METHODS
  The shedding rate was studied in perfused whole rabbit eyes, and the effect of a shear force examined by exposing the corneas to a stirred solution. Control corneas were exposed to a static solution. The shedding rate and size of shed cells were measured, and the number of terminally differentiated cells on the corneal surface determined after 6 h of perfusion using ethidium bromide.
  
  
  RESULTS
  Compared with controls, the shear force increased the cell shedding rate from the corneal surface significantly (p < 0.01, paired t-test). The increase was due to small cells with a longest dimension less than 25 microm. The number of terminally differentiated cells on the epithelial surface did not increase.
  
  
  CONCLUSIONS
  Because of the decrease in size, and the change in appearance of shedding cells, it is proposed that the increase in cell shedding rate was due to an increase in the number of apoptotic cells, and not to an increase in terminally differentiated cells. It is suggested that in the human eye, under adverse conditions, shear forces due to blinking may play a role in creating apoptotic cells.
authors.affiliation: Department of Ophthalmology, University of Texas SW Medical Center, Dallas, USA.
authors.forename: H
authors.initials: H
authors.lastname: Ren
authors.forename: G
authors.initials: G
authors.lastname: Wilson
mesh_terms: D000818:Animals; D002452:Cell Count; D002454:Cell Differentiation; D002470:Cell Survival; D003315:Cornea / Q000166:cytology* / Q000502:physiology*; D004847:Epithelial Cells / Q000166:cytology / Q000502:physiology; D011817:Rabbits; D013314:Stress, Mechanical