Altered N-glycosylation of glucose transporter-1 associated with radiation-induced tumorigenesis of human cell hybrids.
Studies on human cell hybrids between a cervical carcinoma cell line, HeLa, and normal fibroblasts have indicated that their tumorigenicity is under the control of a putative tumor suppressor on chromosome 11. We have previously demonstrated that a tumorigenic cell hybrid CGL4 expresses a larger glucose transporter, GLUT1, due to altered glycosylation when compared to the nontumorigenic counterpart CGL1. In this study, we demonstrated this glycosylation change in GLUT1 in gamma-ray-induced tumorigenic mutants (GIMs) isolated from CGL1 cells as expressing a tumor-associated surface antigen, intestinal alkaline phosphatase. In contrast, GLUT1 in the gamma-irradiated nontumorigenic control cells (CONs) did not show this alteration. In accordance with this glycosylation change, affinity to 2-deoxyglucose in these GIM clones was increased by about twofold when compared to the nontumorigenic CONs. These results suggest a close correlation between the glycosylation change in GLUT1 with increased affinity to D-glucose and tumorigenicity of these human cell hybrids.
Noto
Y
Y
Department of Biochemistry and Cell Biology, National Institute of Infectious Diseases, Tokyo, Japan.
Iwazaki
A
A
Nagao
J
J
Sumiyama
Y
Y
Redpath
J L
JL
Stanbridge
E J
EJ
Kitagawa
T
T
D000469:Alkaline Phosphatase / Q000378:metabolism; D001692:Biological Transport; D002471:Cell Transformation, Neoplastic / Q000528:radiation effects*; D003847:Deoxyglucose / Q000378:metabolism; D005347:Fibroblasts; D005720:Gamma Rays; D051272:Glucose Transporter Type 1; D006031:Glycosylation / Q000528:radiation effects; D006367:HeLa Cells; D006801:Humans; D006822:Hybrid Cells; D007700:Kinetics; D009004:Monosaccharide Transport Proteins / Q000737:chemistry / Q000378:metabolism* / Q000528:radiation effects; D009844:Oligosaccharides / Q000737:chemistry / Q000302:isolation & purification
D051272:Glucose Transporter Type 1; D009004:Monosaccharide Transport Proteins; D009844:Oligosaccharides; C495677:SLC2A1 protein, human; D003847:Deoxyglucose; D000469:Alkaline Phosphatase