[The regulation of the secretion of transforming growth factor-beta 1 by estrogen on ovarian cancer AO, 3AO cell lines and its relationship with cell proliferation].
OBJECTIVE
To investigate the regulatory effect of the secretion of transforming growth factor-beta 1 (TGF-beta 1) by estrogen on ovarian cancer AO, 3AO cell lines and on cell proliferation.


METHODS
The secretion of TGF-beta 1 was investigated by using western dot blot and the cell proliferation by 3H-thymidine uptake.


RESULTS
In vitro, AO cells under the effect of estrogen can secreted TGF-beta 1, and there was cell proliferation, but both gave dose-dependent responses. Estrogen significantly promoted the secretion of TGF-beta 1 of AO cells at low (10(-13) mol/L) and high (10(-5)mol/L) concentrations, but the level of cell proliferation showed inhibition. In contrast, in the 3AO cells under the same conditions, no difference in TGF-beta 1 secretion was found from those of the control groups, dose-dependent response was seen in cell proliferation and there was no significant correlation between the expression of TGF-beta 1 and cellular proliferation.


CONCLUSIONS
The results suggest that there may be TGF-beta 1 autocrine loop in AO ovarian cancer cell line, which, when estrogen regulated may be associated with the cell proliferation. In 3AO cell line, under the same conditions, TGF-beta 1, autocrine is not regulated by estrogen, nor is it related to cell proliferation regulation.
Dai
J
J
Obstetric and Gynecological Hospital of Shanghai Medical University.
Feng
Y
Y
Yang
Y
Y
D002455:Cell Division / Q000187:drug effects; D018284:Cystadenocarcinoma, Serous / Q000378:metabolism* / Q000473:pathology; D004967:Estrogens / Q000494:pharmacology*; D005260:Female; D006801:Humans; D010051:Ovarian Neoplasms / Q000378:metabolism* / Q000473:pathology; D016212:Transforming Growth Factor beta / Q000378:metabolism*; D014407:Tumor Cells, Cultured
D004967:Estrogens; D016212:Transforming Growth Factor beta