An electrophoretic molecular karyotype of a clinical isolate of Aspergillus fumigatus and localization of the MDR-like genes AfuMDR1 and AfuMDR2.
The molecular karyotype of a clinical isolate of Aspergillus fumigatus (10AF/86/10) was determined by contour-clamped homogeneous electric field gel electrophoresis. Five chromosomal bands were resolved by this method. The resolved chromosomes ranged in size from 1.7 to 4.8 Mb, and together constituted a total genomic size of at least 15.8 Mb. Southern analysis of the separated chromosomes located the position of two MDR-like genes, AfuMDR1 and AfuMDR2, on chromosomes III and IV, respectively. The methods described herein may enable the application of molecular karyotyping of A. fumigatus in epidemiologic surveillance studies.
Tobin
M B
MB
Department of Infectious Diseases, Lilly Research Laboratories, Eli Lilly and Company, Indianapolis, Indiana 46285, USA.
Peery
R B
RB
Skatrud
P L
PL
D001228:Aspergillosis; D001232:Aspergillus fumigatus / Q000235:genetics* / Q000302:isolation & purification; D015342:DNA Probes; D004271:DNA, Fungal / Q000032:analysis*; D016521:Electrophoresis, Gel, Pulsed-Field; D015870:Gene Expression; D019450:Genes, MDR; D006801:Humans; D007621:Karyotyping; D012333:RNA, Messenger / Q000032:analysis*; D012680:Sensitivity and Specificity
D015342:DNA Probes; D004271:DNA, Fungal; D012333:RNA, Messenger