Salmonella infection increases porcine antibacterial peptide concentrations in serum.
PR-39 is a multifunctional neutrophil peptide involved in host defense and inflammation. To investigate the involvement of PR-39 in a Salmonella choleraesuis infection, a PR-39 enzyme immunoassay was developed. The concentrations of PR-39 in serum were 13.6 +/- 1.9 ng/ml before challenge and increased (P < 0.01) threefold by 10 to 14 days postinfection. Peripheral blood neutrophil counts paralleled the changes in the concentrations of PR-39 in serum, both returned to basal values by 4 weeks postinfection. These findings suggest that the concentrations of serum PR-39 reflect the involvement of this antibacterial peptide in the host's response to an S. choleraesuis infection.
Zhang
G
G
Department of Anatomy and Physiology, Kansas State University, Manhattan 66506, USA.
Ross
C R
CR
Dritz
S S
SS
Nietfeld
J C
JC
Blecha
F
F
D000818:Animals; D000900:Anti-Bacterial Agents / Q000097:blood*; D023181:Antimicrobial Cationic Peptides; D001770:Blood Bactericidal Activity; D004797:Enzyme-Linked Immunosorbent Assay; D006041:Goats; D051379:Mice; D008807:Mice, Inbred BALB C; D018375:Neutrophil Activation / Q000187:drug effects / Q000502:physiology; D009504:Neutrophils / Q000187:drug effects / Q000378:metabolism; D010455:Peptides / Q000097:blood*; D012481:Salmonella Infections, Animal / Q000097:blood* / Q000276:immunology; D013268:Stimulation, Chemical; D013552:Swine; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology
D000900:Anti-Bacterial Agents; D023181:Antimicrobial Cationic Peptides; D010455:Peptides; C072212:PR 39; D013755:Tetradecanoylphorbol Acetate