title: [Study on the distribution and origins of RNA in the metaphase chromosomes of mouse by in situ hybridization at electron microscopic level].
abstract: The RNAs in the metaphase chromosomes of mouse are located by use of RNase-gold labelling. The result shows that a lot of RNAs distribute uniformly in the inner and peripheral regions of the chromosome sections. Moreover, the distributions of 18 s rRNA and 5 s rRNA which are studied under the electron microscope by in situ hybridization indicate that 18 s rRNA and 5 s rRNA are two among the several origins of the inner RNAs and peripheral RNAs in mouse metaphase chromosomes.
authors.affiliation: Biochem and Molecular Bio Dept., Nankai University, Tianjin.
authors.forename: Y
authors.initials: Y
authors.lastname: Mei
authors.forename: Z L
authors.initials: ZL
authors.lastname: Zhang
authors.forename: T Y
authors.initials: TY
authors.lastname: Fan
authors.forename: S Y
authors.initials: SY
authors.lastname: Guo
authors.forename: X L
authors.initials: XL
authors.lastname: Wang
mesh_terms: D000818:Animals; D002466:Cell Nucleolus / Q000737:chemistry; D002875:Chromosomes / Q000235:genetics* / Q000648:ultrastructure; D017403:In Situ Hybridization; D008677:Metaphase; D051379:Mice; D008854:Microscopy, Electron; D012337:RNA, Ribosomal, 18S / Q000032:analysis*; D012341:RNA, Ribosomal, 5S / Q000032:analysis*
chemical_list: D012337:RNA, Ribosomal, 18S; D012341:RNA, Ribosomal, 5S