title: c-di-GMP-binding protein, a new factor regulating cellulose synthesis in Acetobacter xylinum.
abstract: A protein which specifically binds cyclic diguanylic acid (c-di-GMP), the reversible allosteric activator of the membrane-bound cellulose synthase system of Acetobacter xylinum, has been identified in membrane preparations of this organism. c-di-GMP binding is of high affinity (KD 20 nM), saturable and reversible. The equilibrium of the reaction is markedly and specifically shifted towards the binding direction by K+. The c-di-GMP binding protein, structurally associated with the cellulose synthase, appears to play a major role in modulating the intracellular concentration of free c-di-GMP and thus may constitute an essential factor in regulating cellulose synthesis in vivo.
authors.affiliation: Department of Biological Chemistry, Institute of Life Sciences, Hebrew University of Jerusalem, Givat Ram, Israel.
authors.forename: H
authors.initials: H
authors.lastname: Weinhouse
authors.forename: S
authors.initials: S
authors.lastname: Sapir
authors.forename: D
authors.initials: D
authors.lastname: Amikam
authors.forename: Y
authors.initials: Y
authors.lastname: Shilo
authors.forename: G
authors.initials: G
authors.lastname: Volman
authors.forename: P
authors.initials: P
authors.lastname: Ohana
authors.forename: M
authors.initials: M
authors.lastname: Benziman
mesh_terms: D000494:Allosteric Regulation; D001426:Bacterial Proteins / Q000302:isolation & purification / Q000378:metabolism*; D002352:Carrier Proteins / Q000302:isolation & purification / Q000378:metabolism*; D002482:Cellulose / Q000096:biosynthesis*; D002850:Chromatography, Gel; D006152:Cyclic GMP / Q000031:analogs & derivatives* / Q000378:metabolism; D004734:Energy Metabolism / Q000187:drug effects; D004789:Enzyme Activation; D004983:Ethanolamines / Q000494:pharmacology; D041822:Gluconacetobacter xylinus / Q000378:metabolism*; D005964:Glucosyltransferases / Q000378:metabolism; D007700:Kinetics; D011188:Potassium / Q000494:pharmacology
chemical_list: D001426:Bacterial Proteins; D002352:Carrier Proteins; D004983:Ethanolamines; C062025:bis(3',5')-cyclic diguanylic acid; D002482:Cellulose; C020283:diethanolamine; D005964:Glucosyltransferases; C076062:cellulose synthase (cyclic diguanylic acid); D006152:Cyclic GMP; D011188:Potassium