Requirement for Valpha14 NKT cells in IL-12-mediated rejection of tumors.
A lymphocyte subpopulation, the Valpha14 natural killer T (NKT) cells, expresses both NK1.1 and a single invariant T cell receptor encoded by the Valpha14 and Jalpha281 gene segments. Mice with a deletion of the Jalpha281 gene segment were found to exclusively lack this subpopulation. The Valpha14 NKT cell-deficient mice could no longer mediate the interleukin-12 (IL-12)-induced rejection of tumors. Although the antitumor effect of IL-12 was thought to be mediated through natural killer cells and T cells, Valpha14 NKT cells were found to be an essential target of IL-12, and they mediated their cytotoxicity by an NK-like effector mechanism after activation with IL-12.
Cui
J
J
Core Research for Evolutional Science and Technology (CREST) Project, Japan Science and Technology Corporation (JST), 1-8-1 Inohana, Chuo-ku, Chiba, Japan 260.
Shin
T
T
Kawano
T
T
Sato
H
H
Kondo
E
E
Toura
I
I
Kaneko
Y
Y
Koseki
H
H
Kanno
M
M
Taniguchi
M
M
D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology; D003602:Cytotoxicity, Immunologic; D017353:Gene Deletion; D018390:Gene Targeting; D016654:Genes, RAG-1; D019673:Genes, T-Cell Receptor alpha; D007371:Interferon-gamma / Q000276:immunology; D018664:Interleukin-12 / Q000276:immunology*; D007694:Killer Cells, Natural / Q000276:immunology*; D018942:Macrolides; D008546:Melanoma, Experimental / Q000276:immunology; D051379:Mice; D008810:Mice, Inbred C57BL; D008822:Mice, Transgenic; D009374:Neoplasms, Experimental / Q000276:immunology*; D011070:Poly I-C / Q000494:pharmacology; D006180:Proton-Translocating ATPases / Q000037:antagonists & inhibitors; D016693:Receptors, Antigen, T-Cell, alpha-beta / Q000235:genetics / Q000276:immunology*; D016176:T-Lymphocyte Subsets / Q000276:immunology*
D000900:Anti-Bacterial Agents; D018942:Macrolides; D016693:Receptors, Antigen, T-Cell, alpha-beta; D018664:Interleukin-12; C036978:concanamycin A; D007371:Interferon-gamma; D006180:Proton-Translocating ATPases; D011070:Poly I-C