Membrane stretch activates a potassium channel in pig articular chondrocytes.
Activity of stretch-activated potassium channels has been recorded in articular chondrocytes using patch-clamp technique. Pressure dependence is described by a sigmoidal function with a half-maximum effect at -20.5 mbar. Selectivity for potassium is demonstrated by agreement between the reversal potential measured at different [K+]o and the prediction of Nernst equation and by block of these channels by caesium.
Martina
M
M
Biophysics Sector, International School for Advanced Studies (SISSA), Trieste, Italy.
Mozrzymas
J W
JW
Vittur
F
F
D000818:Animals; D002358:Cartilage, Articular / Q000502:physiology*; D002462:Cell Membrane / Q000187:drug effects / Q000502:physiology; D002478:Cells, Cultured; D002586:Cesium / Q000494:pharmacology; D015640:Ion Channel Gating; D008564:Membrane Potentials / Q000187:drug effects / Q000502:physiology; D018408:Patch-Clamp Techniques; D011188:Potassium / Q000494:pharmacology; D015221:Potassium Channels / Q000187:drug effects / Q000502:physiology*; D013314:Stress, Mechanical; D013552:Swine
D015221:Potassium Channels; D002586:Cesium; D011188:Potassium