[A study on the change of myocardial neuropeptide Y release induced by electric stimulation and myocardial ischemia in guinea pig heart].
Electrical stimulation of the left stellate ganglion in guinea pig heart evoked a calcium-dependent, exocytotic release of neuropeptide Y (NPY). Stimulation after 10 min of global ischemia (S2), compared with control period stimulation (S1), had no significant effect on the NPY release. The release of NPY produced by the same stimulation after 20 min of ischemia was inhibited to certain extent (S2/S1: 0.72, P < 0.05), whereas the inhibition of NPY release disappeared after 5 min of reperfusion (with a S2/S1 of 1.01). Ischemia alone, without electric stimulation, did not apparently induce NPY release, suggesting that electrical stimulation may induce a calcium-dependent, exocytotic release of NPY. It is further suggested that the inhibition of NPY release may be produced by some metabolites and the abolishment of the inhibition after reperfusion may be due to washout of the metabolites.
Cheng
B
B
Institute of Cardiology, Xiehe Clinic, Tongji Medical University, Wuban.
Xiang
J Z
JZ
Guo
L J
LJ
Hu
B R
BR
Han
Q D
QD
Shi
G P
GP
D000818:Animals; D002118:Calcium / Q000378:metabolism; D004558:Electric Stimulation; D006168:Guinea Pigs; D015428:Myocardial Reperfusion Injury / Q000378:metabolism*; D009206:Myocardium / Q000378:metabolism*; D009478:Neuropeptide Y / Q000378:metabolism*; D013233:Stellate Ganglion / Q000502:physiology
D009478:Neuropeptide Y; D002118:Calcium