Organization and structure of the human tissue transglutaminase gene.
Analysis of the genomic organization of tissue transglutaminase shows that the gene is 32.5 kilobases, contains 13 exons and 12 introns. Our results show that the sites for the two alternative splicing forms of tissue transglutaminase we reported earlier are located within exons 6 and 10 respectively. The 5'-upstream region of the gene has several potential regulatory promoter elements, and the 3'-exon contains about 50% of the total cDNA size and codes for the C-terminus of the protein. Alignment of deduced tissue transglutaminase amino acid sequence with other transglutaminases showed very similar intron splice positions.
Fraij
B M
BM
Department of Biochemistry and Molecular Biology, Oklahoma State University, Stillwater 74078, USA. bmfraij@bioch.okstate.edu
Gonzales
R A
RA
D017398:Alternative Splicing; D000595:Amino Acid Sequence; D004247:DNA; D005091:Exons; D006801:Humans; D007438:Introns; D008969:Molecular Sequence Data; D011401:Promoter Regions, Genetic; D012045:Regulatory Sequences, Nucleic Acid; D017386:Sequence Homology, Amino Acid; D011503:Transglutaminases / Q000235:genetics*
D004247:DNA; D011503:Transglutaminases