Targeting of HIV- and SIV-infected cells by CD4-chemokine receptor pseudotypes.
Retroviral vectors containing CD4 and an appropriate chemokine receptor were evaluated for the ability to transduce cells infected with human immunodeficiency virus (HIV) and simian immunodeficiency virus (SIV). These CD4-chemokine receptor pseudotypes were able to target HIV- and SIV-infected cell lines and monocyte-derived macrophages in a manner that corresponded to the specificity of the viral envelope glycoprotein for its CD4-chemokine receptor complex. This approach could offer a way to deliver antiviral genes directly to HIV-infected cells in vivo and could provide an additional treatment strategy in conjunction with existing antiviral therapies.
Endres
M J
MJ
Department of Medicine, Hematology-Oncology Division, University of Pennsylvania, Philadelphia, PA 19104, USA. endres@mail.med.upenn.edu
Jaffer
S
S
Haggarty
B
B
Turner
J D
JD
Doranz
B J
BJ
O'Brien
P J
PJ
Kolson
D L
DL
Hoxie
J A
JA
D000818:Animals; D015704:CD4 Antigens / Q000235:genetics* / Q000378:metabolism; D002460:Cell Line; D015686:Gene Products, env / Q000378:metabolism; D018014:Gene Transfer Techniques; D005822:Genetic Vectors; D015497:HIV-1 / Q000502:physiology*; D006801:Humans; D008264:Macrophages / Q000821:virology; D010957:Plasmids; D019713:Receptors, CCR5 / Q000235:genetics / Q000378:metabolism; D019718:Receptors, CXCR4 / Q000235:genetics / Q000378:metabolism; D019707:Receptors, Chemokine / Q000235:genetics* / Q000378:metabolism; D015302:Simian Immunodeficiency Virus / Q000502:physiology*; D014162:Transfection
D015704:CD4 Antigens; D015686:Gene Products, env; D019713:Receptors, CCR5; D019718:Receptors, CXCR4; D019707:Receptors, Chemokine