title: Ca2+/Mg(2+)-dependent endonuclease in marrow CD34 positive and erythroid cells in myelodysplasia.
abstract: Endonucleases capable of producing internucleosomal DNA cleavage are one of the key enzymes in apoptosis. We examined endonuclease activities contained in nuclei of CD34+ and erythroid cells in the bone marrow (BM) from 12 patients with the myelodysplastic syndromes. The levels of Mg(2+)-dependent and acidic endonucleases showed little changes as compared with those from normal BM. By contrast, the level of Ca2+/Mg(2+)-dependent endonuclease was appreciably higher in MDS erythroid cells than normal counterparts, although the activity varied markedly in CD34+ and erythroid cells. Our results suggested that Ca2+/Mg(2+)-dependent endonuclease is related to ineffective erythropoiesis in MDS.
authors.affiliation: Department of Hematology and Oncology, Graduate School of Medicine, Kyoto University, Japan.
authors.forename: N
authors.initials: N
authors.lastname: Anzai
authors.forename: H
authors.initials: H
authors.lastname: Kawabata
authors.forename: T
authors.initials: T
authors.lastname: Hishita
authors.forename: Y
authors.initials: Y
authors.lastname: Yoshida
authors.forename: Y
authors.initials: Y
authors.lastname: Ueda
authors.forename: M
authors.initials: M
authors.lastname: Okuma
mesh_terms: D000328:Adult; D000368:Aged; D018952:Antigens, CD34 / Q000032:analysis*; D004706:Endodeoxyribonucleases / Q000378:metabolism*; D015672:Erythroid Precursor Cells / Q000201:enzymology*; D005260:Female; D006412:Hematopoietic Stem Cells / Q000201:enzymology*; D006801:Humans; D008297:Male; D008875:Middle Aged; D009190:Myelodysplastic Syndromes / Q000097:blood / Q000201:enzymology*
chemical_list: D018952:Antigens, CD34; D004706:Endodeoxyribonucleases; C029367:calcium magnesium dependent endodeoxyribonuclease