Genetic mapping of 262 loci derived from expressed sequences in a murine interspecific cross using single-strand conformational polymorphism analysis.
We have demonstrated previously that noncoding sequences of genes are a robust source of polymorphisms between mouse species when tested using single-strand conformation polymorphism (SSCP) analysis, and that these polymorphisms are useful for genetic mapping. In this report we demonstrate that presumptive 3'-untranslated region sequence obtained from expressed sequence tags (ESTs) can be analyzed in a similar fashion, and we have used this approach to map 262 loci using an interspecific backcross. These results demonstrate SSCP analysis of genes or ESTs is a simple and efficient means for the genetic localization of transcribed sequences, and is furthermore an approach that is applicable to any system for which there is sufficient sequence polymorphism.
Brady
K P
KP
Division of Genetics, Brigham and Women's Hospital, Harvard Medical School, Boston, Massachusetts 02115, USA.
Rowe
L B
LB
Her
H
H
Stevens
T J
TJ
Eppig
J
J
Sussman
D J
DJ
Sikela
J
J
Beier
D R
DR
D000818:Animals; D002874:Chromosome Mapping; D003433:Crosses, Genetic; D004252:DNA Mutational Analysis / Q000379:methods; D015723:Gene Library; D005819:Genetic Markers; D051379:Mice; D008806:Mice, Inbred AKR / Q000235:genetics; D008809:Mice, Inbred C3H / Q000235:genetics; D008810:Mice, Inbred C57BL / Q000235:genetics; D008811:Mice, Inbred DBA / Q000235:genetics; D008815:Mice, Inbred Strains / Q000235:genetics*; D009115:Muridae / Q000235:genetics*; D018807:Polymorphism, Single-Stranded Conformational; D016324:Sequence Tagged Sites; D014158:Transcription, Genetic
D005819:Genetic Markers