title: Deletion mapping and linkage analysis provide strong indication for the involvement of the human chromosome region 8p12-p22 in breast carcinogenesis.
abstract: We have identified a high frequency of loss of heterozygosity (LOH) on the human chromosome region 8p12-p22 in a panel of microdissected familial (86% LOH) and sporadic (74% LOH) breast tumours. The two most frequently deleted regions were defined around marker D8S133 and in a broader centromeric region bounded by markers D8S137 and D8S339. We cannot unequivocally characterize the 8p12-p22 loss as an early or a late event in breast carcinogenesis. In parallel, we have performed linkage analysis in four German breast cancer families. A location score greater than 13.67 corresponding to a LOD score of 2.97 at the marker D8S137 has been obtained. Our results considerably strengthen the evidence for a breast cancer susceptibility gene(s) located on the short arm of the chromosome region at 8p12-p22.
authors.affiliation: Department of Tumour Genetics, Max Delbrueck Center for Molecular Medicine Berlin, Germany.
authors.forename: S
authors.initials: S
authors.lastname: Seitz
authors.forename: K
authors.initials: K
authors.lastname: Rohde
authors.forename: E
authors.initials: E
authors.lastname: Bender
authors.forename: A
authors.initials: A
authors.lastname: Nothnagel
authors.forename: H
authors.initials: H
authors.lastname: Pidde
authors.forename: O M
authors.initials: OM
authors.lastname: Ullrich
authors.forename: A
authors.initials: A
authors.lastname: El-Zehairy
authors.forename: W
authors.initials: W
authors.lastname: Haensch
authors.forename: B
authors.initials: B
authors.lastname: Jandrig
authors.forename: K
authors.initials: K
authors.lastname: Kölble
authors.forename: P M
authors.initials: PM
authors.lastname: Schlag
authors.forename: S
authors.initials: S
authors.lastname: Scherneck
mesh_terms: D000328:Adult; D000368:Aged; D001943:Breast Neoplasms / Q000235:genetics* / Q000473:pathology; D002874:Chromosome Mapping; D002898:Chromosomes, Human, Pair 8; D005192:Family Health; D005260:Female; D017353:Gene Deletion; D008040:Genetic Linkage; D006801:Humans; D019656:Loss of Heterozygosity; D018895:Microsatellite Repeats; D008875:Middle Aged