title: Influences of dietary methionine and cysteine on metabolic responses to immunological stress by Escherichia coli lipopolysaccharide injection, and mitogenic response in broiler chickens.
abstract: The present experiments were conducted to investigate influences of dietary methionine and cysteine on metabolic responses to immunological stress induced by Escherichia coli lipopolysaccharide (LPS) injection, and concanavalin A (Con A)-induced mononuclear cell (MNC) proliferation in male broiler chickens. In Expt 1, chicks (12 d of age) were fed on a S amino acid (SAA)-deficient diet (5.6 g SAA/kg diet) or on three kinds of SAA-sufficient diet (9.3 g SAA/kg diet; low-, medium- and high-cysteine diets) which contained 2.8, 4.65 and 6.5 g cysteine/kg diet, respectively. Plasma alpha-1 acid glycoprotein (AGP) concentration and interleukin (IL)-1-like activity in chicks fed on the SAA-deficient diet were lower following a single injection of LPS than those in chicks fed on the SAA-sufficient diets. At 16 h after LPS injection, plasma Fe and Zn concentrations and body weight were reduced, but AGP concentration and IL-1-like activity in plasma were significantly increased. These changes in body weight, plasma Zn and Fe concentrations following injection of LPS were not affected by dietary methionine: cysteine ratios. Plasma AGP concentration and IL-1-like activity in chicks fed on the high-cysteine diet were, however, greater than those in chicks fed on the other diets following a single injection of LPS. In Expt 2, chicks (7 d of age) were fed on the SAA-sufficient diets as in Expt 1 for 10 d. MNC proliferation in spleen induced by Con A in chicks fed on the high-cysteine diet was greater than that in chicks fed on the low- or medium-cysteine diet. The results suggest that dietary cysteine has an impact on the immune and inflammatory responses.
authors.affiliation: Department of Animal Science, Faculty of Agriculture, Tohoku University, Sendai-shi, Japan.
authors.forename: K
authors.initials: K
authors.lastname: Takahashi
authors.forename: N
authors.initials: N
authors.lastname: Ohta
authors.forename: Y
authors.initials: Y
authors.lastname: Akiba
mesh_terms: D000210:Acute-Phase Reaction; D000596:Amino Acids / Q000008:administration & dosage*; D000818:Animals; D001835:Body Weight; D002645:Chickens / Q000276:immunology* / Q000378:metabolism; D003208:Concanavalin A / Q000494:pharmacology; D003545:Cysteine / Q000008:administration & dosage; D004032:Diet; D004926:Escherichia coli; D007375:Interleukin-1 / Q000378:metabolism; D007501:Iron / Q000097:blood; D007963:Leukocytes, Mononuclear / Q000187:drug effects; D008070:Lipopolysaccharides / Q000008:administration & dosage*; D008297:Male; D008715:Methionine / Q000008:administration & dosage; D008934:Mitogens / Q000494:pharmacology; D011897:Random Allocation; D013154:Spleen / Q000276:immunology; D013950:Thymus Gland / Q000276:immunology; D015032:Zinc / Q000097:blood
chemical_list: D000596:Amino Acids; D007375:Interleukin-1; D008070:Lipopolysaccharides; D008934:Mitogens; D003208:Concanavalin A; D008715:Methionine; D007501:Iron; D015032:Zinc; D003545:Cysteine