Collection and recruitment of CD34+ cells during large-volume leukapheresis.
Although sufficient progenitor cells for hematopoietic rescue following high-dose therapy may be obtained in a single leukapheresis, the majority of patients require multiple procedures. In an attempt to minimize the number of leukapheresis and maximize collection efficiency, we undertook large-volume leukapheresis in 17 patients with a variety of hematologic malignancies. Twenty-four procedures were performed over a 6-h period, with a mean of 21 L of blood processed. By employing a modified collection set, three separate 2-h collection bags were analyzed for a number of variables. CD34+ cells are collected at a steady rate throughout the procedure, with no evidence of exhaustion of progenitor cells. There was evidence of progenitor cell recruitment, with 1.4-fold more CD34+ cells in the collected product than were present in the blood at the beginning of the procedure. Initiation of leukapheresis was based on the blood CD34+ count, and this value was strongly correlated with the number of CD34+ cells in the collected product. The procedure is safe and relatively simple and minimizes the number of leukaphereses required to collect adequate progenitors for autologous transplantation.
Cull
G
G
Haematology Department, Royal Perth Hospital, Australia.
Ivey
J
J
Chase
P
P
Picciuto
R
R
Herrmann
R
R
Cannell
P
P
D000293:Adolescent; D000328:Adult; D018952:Antigens, CD34 / Q000097:blood*; D001800:Blood Specimen Collection / Q000379:methods*; D001810:Blood Volume; D005260:Female; D019650:Hematopoietic Stem Cell Mobilization; D006412:Hematopoietic Stem Cells / Q000276:immunology*; D006801:Humans; D007700:Kinetics; D007937:Leukapheresis / Q000009:adverse effects / Q000379:methods*; D008297:Male; D008875:Middle Aged
D018952:Antigens, CD34