Isocitrate lyase localisation in Saccharomyces cerevisiae cells.
The isocitrate lyase from Saccharomyces cerevisiae was only located in the cell cytoplasm. This protein was found not to be associated with cell organelles, even under growth conditions that induce peroxisome proliferation. This conclusion is supported by experiments carried out by damaging the protoplast plasma membrane with DEAE-dextran, by differential centrifugation of osmotically lysed protoplast and by using the green fluorescent protein (GFP) of Aequorea victoria as a reporter fusion tag to localise the subcellular compartment to which isocitrate lyase is targeted.
Chaves
R S
RS
Departamento de Bioquímica y Biología Molecular, Instituto Universitario de Biotecnología de Asturias, Universidad de Oviedo, Spain.
Herrero
P
P
Ordiz
I
I
Angeles del Brio
M
M
Moreno
F
F
D002451:Cell Compartmentation; D003593:Cytoplasm / Q000201:enzymology; D007522:Isocitrate Lyase / Q000378:metabolism*; D008830:Microbodies / Q000201:enzymology; D012441:Saccharomyces cerevisiae / Q000201:enzymology*
D007522:Isocitrate Lyase