Micellar electrokinetic capillary chromatography combined with immunoaffinity chromatography for identification and determination of dexamethasone and flumethasone in equine urine.
A capillary electrophoresis technique was developed for the separation of synthetic glucocorticoids and the determination of dexamethasone and flumethasone in horse urine. Pretreatment of the sample using a dexamethasone affinity column resulted in low background that enabled the authors to detect levels as low as 1.1 ng/mL and 2.7 ng/mL for dexamethasone and flumethasone in horse urine, respectively. The developed method was used to detect dexamethasone in horse urine samples after the injection of a therapeutic dose of dexamethasone for up to 12 hr postinjection. The optimum conditions for capillary electrophoresis and dexamethasone elution from the affinity column are described.
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Department of Large Animal Clinical Sciences, College of Veterinary Medicine, University of Florida, Gainesville 32610-0136, USA.
Meleka-Boules
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Chen
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D000818:Animals; D000906:Antibodies; D002197:Capillary Action; D002845:Chromatography / Q000295:instrumentation / Q000379:methods; D002846:Chromatography, Affinity / Q000295:instrumentation / Q000379:methods; D002851:Chromatography, High Pressure Liquid; D003907:Dexamethasone / Q000302:isolation & purification / Q000493:pharmacokinetics / Q000652:urine*; D019075:Electrophoresis, Capillary / Q000295:instrumentation / Q000379:methods; D005260:Female; D005443:Flumethasone / Q000302:isolation & purification / Q000652:urine*; D005938:Glucocorticoids / Q000302:isolation & purification / Q000652:urine*; D006736:Horses; D007074:Immunoglobulin G; D008823:Micelles; D011817:Rabbits; D011863:Radioimmunoassay; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
D000906:Antibodies; D005938:Glucocorticoids; D007074:Immunoglobulin G; D008823:Micelles; D003907:Dexamethasone; D005443:Flumethasone