[Effects of acetylcholine, glycine and their antagonist on spontaneous discharge of neurons in nucleus paragigantocellularis lateralis in rats].
The effects of iontophoretic application of acetylcholine (ACh), glycine (Gly) and their antagonist atropine, strychnine on the spontaneous discharge of the neurons in the caudal half of the nucleus paragigantocellularis lateralis (cPGCL) and the influence of atropine and strychnine on the effects of ACh and Gly respectively were observed on 17 anesthetized spontaneously breathing Sprague-Dawley rats. ACh could excite 79.6% of the tested nuerons. Gly could inhibit 91.5% of the tested nuerons. Atropine could excite 8% and inhibit 48% of the tested neurons. Strychnine could excite 21.9% and inhibite 6.3% of the tested nuerons. The excitatory effect of ACh and the inhibitory effect of Gly and atropine were dose-dependent. In most of the tested nuerons, the excitatory response to ACh (80.3%) and the inhibitory response to Gly (92.6%) could be blocked by their antagonists atropine and strychnine respectively. The results suggest that there exist endogenous ACh and Gly in the cPGCL and M receptors and Gly receptors on some of neurons cPGCL. These neurotransmitters and neuromodulators and receptors might be involved in the regulation of respiratory and cardiovascular function by cPGCL.
Yu
W
W
Department of Physiology, Research Unit of Neurophysiology, School of Basic Medical Sciences, Chengdu.
Zheng
Y
Y
Xu
M
M
D000109:Acetylcholine / Q000037:antagonists & inhibitors*; D000818:Animals; D001285:Atropine / Q000494:pharmacology; D004594:Electrophysiology; D005260:Female; D005998:Glycine / Q000037:antagonists & inhibitors*; D018684:Glycine Agents / Q000494:pharmacology; D008297:Male; D008526:Medulla Oblongata / Q000502:physiology*; D018727:Muscarinic Antagonists / Q000494:pharmacology; D009474:Neurons / Q000502:physiology; D051381:Rats; D017207:Rats, Sprague-Dawley; D013331:Strychnine / Q000494:pharmacology
D018684:Glycine Agents; D018727:Muscarinic Antagonists; D001285:Atropine; D013331:Strychnine; D000109:Acetylcholine; D005998:Glycine