[Subclones of fragment DNA recombinant rpDJH2 of L. interrogans serovar lai strain 017 and it's expression of high level in E. coli].
Fragment of 1.9 kb recombinant DNA of pDJH2 was linked with vectors pT7-7 and pRSETs. Then they were transformed into E. coli JM109 (DE3) respectively. Expression of subclones was achieved in E. coli JM109 (DE3) with IPTG inducement. SDS-PAGE showed that the molecular weights of products were 68kd and 23 kd respectively. The amount of production seemed to be higher than that of the outer membrane proteins of L. interrogans serovar strain 017 in nature. Immunoblotting of pDJt and pDJrB2 (both are subclones) with the specific antiserum of anti-OMP of L. interrogans serovar lai strain 017 and the experiment of initiative immuno-protection in guinea pigs showed both protein-68 kd and 23 kd might be the antigens of immuno-protection on the outer membrane of L. interrogans serovar lai strain 017.
Jiang
N
N
West China University of Medical Sciences, Chengdu.
Dai
B
B
Li
S
S
Zhao
H
H
Fang
Z
Z
Wu
W
W
Ye
D
D
Liu
J
J
Song
S
S
Yang
Y
Y
Zhang
Y
Y
Liu
F
F
Tu
Y
Y
Yang
H
H
Huang
Z
Z
Liang
L
L
Hu
L
L
Zhao
M
M
D000818:Animals; D000942:Antigens, Bacterial / Q000096:biosynthesis / Q000235:genetics; D001425:Bacterial Outer Membrane Proteins / Q000235:genetics* / Q000276:immunology; D004274:DNA, Recombinant / Q000096:biosynthesis / Q000235:genetics*; D004926:Escherichia coli / Q000235:genetics*; D015870:Gene Expression; D006168:Guinea Pigs; D006801:Humans; D007921:Leptospira interrogans / Q000235:genetics*; D008297:Male; D010957:Plasmids; D014170:Transformation, Genetic
D000942:Antigens, Bacterial; D001425:Bacterial Outer Membrane Proteins; D004274:DNA, Recombinant