title: Sequence completion, identification and definition of the fengycin operon in Bacillus subtilis 168.
abstract: A 15 kb DNA fragment from the Bacillus subtilis chromosome between citB and ppsC has been sequenced, and new ORFs encoding putative enzymes involved in lipopolypeptide synthesis, which complete a partial operon previously reported, and a new set of enzymes responsible for lipid metabolism have been identified. From the analysis of DNA sequence homology of the fragment it was deduced that these new peptide synthetase genes are part of an operon for the biosynthesis of the fungicide fengycin.
authors.affiliation: Microbiology Group, International Centre for Genetic Engineering and Biotechnology and ITALTB5 Ricerche,Sri, AREA Science Park, Padriciano 99, 1-34012 Trieste,Italy.
authors.forename: Valentina
authors.initials: V
authors.lastname: Tosato
authors.affiliation: Department of Genetics and Microbiology A. Buzzati Traverso, University of Pavia,27100 Pavia,Italy.
authors.forename: Alessandra M
authors.initials: AM
authors.lastname: Albertini
authors.affiliation: Microbiology Group, International Centre for Genetic Engineering and Biotechnology and ITALTB5 Ricerche,Sri, AREA Science Park, Padriciano 99, 1-34012 Trieste,Italy.
authors.forename: Michela
authors.initials: M
authors.lastname: Zotti
authors.affiliation: Microbiology Group, International Centre for Genetic Engineering and Biotechnology and ITALTB5 Ricerche,Sri, AREA Science Park, Padriciano 99, 1-34012 Trieste,Italy.
authors.forename: Sabrina
authors.initials: S
authors.lastname: Sonda
authors.affiliation: Microbiology Group, International Centre for Genetic Engineering and Biotechnology and ITALTB5 Ricerche,Sri, AREA Science Park, Padriciano 99, 1-34012 Trieste,Italy.
authors.forename: Carlo V
authors.initials: CV
authors.lastname: Bruschi
mesh_terms: D000935:Antifungal Agents / Q000096:biosynthesis*; D001412:Bacillus subtilis / Q000201:enzymology / Q000235:genetics*; D005227:Fatty Acids / Q000378:metabolism; D005798:Genes, Bacterial / Q000235:genetics*; D055666:Lipopeptides; D008074:Lipoproteins / Q000096:biosynthesis; D008969:Molecular Sequence Data; D009097:Multienzyme Complexes / Q000235:genetics; D016366:Open Reading Frames / Q000235:genetics; D009876:Operon / Q000235:genetics*; D015183:Restriction Mapping; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid
chemical_list: D000935:Antifungal Agents; D005227:Fatty Acids; D055666:Lipopeptides; D008074:Lipoproteins; D009097:Multienzyme Complexes; C049972:fengycin