Human umbilical cord blood myeloid progenitor cells are relatively chemoresistant: a potential model for autologous transplantations in HIV-infected newborns.
Vertical transmission from mother to child occurs in 15-39% of women infected with the human immunodeficiency virus (HIV). Stem cell transplantation has recently been suggested as a potential therapy for patients with HIV infection. We have examined the possible advantages of human cord blood (HUCB) stem cells over bone marrow (BM) stem cells in the treatment of HIV-infected newborns. HUCB myeloid progenitors were found to be statistically more resistant to interferon-alpha (IFN-alpha), cytarabine (ARA-C), and eilatin than BM myeloid progenitor cells grown with IL-3 (P < 0.05). HUCB treated with IFN-alpha, ARA-C, and eilatin demonstrated a significantly higher capacity for self-renewal manifested by delta assay following 7 days in liquid culture. We, therefore, suggest that HUCB purged by anti-HIV drugs may be a source for autologous transplantation in HIV-infected newborns.
Toren
A
A
Department of Bone Marrow Transplantation, Hadassah University Hospital, Jerusalem, Israel.
Einat
M
M
Fabian
I
I
Nagler
A
A
D000998:Antiviral Agents / Q000494:pharmacology*; D001854:Bone Marrow Cells / Q000187:drug effects; D002455:Cell Division / Q000187:drug effects; D002478:Cells, Cultured; D003114:Colony-Forming Units Assay; D003561:Cytarabine / Q000494:pharmacology; D004351:Drug Resistance; D005260:Female; D005312:Fetal Blood / Q000166:cytology*; D005434:Flow Cytometry; D015658:HIV Infections / Q000628:therapy*; D018380:Hematopoietic Stem Cell Transplantation / Q000379:methods*; D006412:Hematopoietic Stem Cells / Q000187:drug effects*; D006801:Humans; D007231:Infant, Newborn; D016898:Interferon-alpha / Q000494:pharmacology; D008954:Models, Biological; D010618:Phenanthrolines / Q000494:pharmacology; D014182:Transplantation, Autologous; D015215:Zidovudine / Q000494:pharmacology
D000998:Antiviral Agents; D016898:Interferon-alpha; D010618:Phenanthrolines; D003561:Cytarabine; C097538:eilatine; D015215:Zidovudine