title: Estrogen receptor alpha and beta expression in upper gastrointestinal tract with regulation of trefoil factor family 2 mRNA levels in ovariectomized rats.
abstract: This study compared expression of two estrogen receptor (ER alpha and ER beta) genes in the rat upper gastrointestinal tract and the effects of 17 beta-estradiol administration on gastric trefoil factor family (TFF) mRNA steady-state levels in ovariectomized rats. Estrogen receptor alpha and beta cDNA fragments from fundic mucosa were cloned by reverse transcriptase polymerase chain reaction (RT-PCR) and sequenced. Both ER subtypes were detected in fundus, antrum and duodenum by RT-PCR. Northern analysis of poly(A)+ mRNA from fundic mucosa showed that ER alpha mRNA is expressed as a single transcript at 6.5 kb and ER beta is expressed as multiple transcripts with major transcripts ranging from 1.1-4.7 kb. ER beta mRNA was expressed in greater abundance than ER alpha mRNA. Fundic TFF2 mRNA steady-state levels were increased by 17 beta-estradiol administration in ovariectomized rats with no significant change in TFF1 mRNA levels. These studies show expression of both ER subtypes in the rat upper gastrointestinal tract with regulation of TFF2 mRNA by 17 beta-estradiol. These results suggest that estrogens, probably acting via ER beta, have a direct role in regulating gastric physiology.
authors.affiliation: Department of Medicine, College of Medicine, University of Florida, Gainesville 32610-0267, USA. MCT@MEDICINE.UFL.EDU
authors.forename: M L
authors.initials: ML
authors.lastname: Campbell-Thompson
mesh_terms: D000818:Animals; D004386:Duodenum / Q000378:metabolism*; D047628:Estrogen Receptor alpha; D047629:Estrogen Receptor beta; D005260:Female; D005748:Gastric Fundus; D005753:Gastric Mucosa / Q000378:metabolism*; D006133:Growth Substances / Q000378:metabolism*; D007413:Intestinal Mucosa / Q000378:metabolism*; D009077:Mucins; D009124:Muscle Proteins; D009479:Neuropeptides; D010052:Ovariectomy; D010455:Peptides / Q000378:metabolism*; D016133:Polymerase Chain Reaction; D011706:Pyloric Antrum; D012333:RNA, Messenger / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley; D011960:Receptors, Estrogen / Q000096:biosynthesis*; D014158:Transcription, Genetic; D000071164:Trefoil Factor-2; D000071165:Trefoil Factor-3; D014599:Uterus / Q000378:metabolism*
chemical_list: D047628:Estrogen Receptor alpha; D047629:Estrogen Receptor beta; D006133:Growth Substances; D009077:Mucins; D009124:Muscle Proteins; D009479:Neuropeptides; D010455:Peptides; D012333:RNA, Messenger; D011960:Receptors, Estrogen; C099037:TFF3 protein, rat; C000604008:Tff2 protein, rat; D000071164:Trefoil Factor-2; D000071165:Trefoil Factor-3