title: Characterization of the equine glycoprotein hormone alpha-subunit gene reveals divergence in the mechanism of pituitary and placental expression.
abstract: The equine glycoprotein hormone alpha-subunit gene is expressed in both pituitary and placenta, unlike that of all other nonprimate mammals studied, in which expression is limited to pituitary. Previous studies of the 5'-flanking region of the equine alpha-subunit promoter have revealed unique characteristics as well as similarities with the human alpha-subunit promoter, which demonstrates a similar pattern of tissue-specific expression. We have cloned and sequenced the equine alpha-subunit gene and have used tissue culture systems and transgenic mice to characterize its expression. Unlike the human promoter, the cloned equine alpha-subunit promoter failed to direct trophoblast-specific expression in either tissue culture or transgenic mouse models, suggesting an entirely different mechanism for expression. In contrast, the equine alpha-subunit promoter was able to direct gonadotroph expression in both tissue culture and transgenic mouse models. In alphaT3-1 cells, 550 base pair (bp) was sufficient for expression. This expression involves promoter elements identified in other species as playing a role in gonadotroph expression, but mutation of these elements reveals differences in their relative contributions to promoter activity. In mice, 2800 bp of 5'-flanking sequence allowed specific expression in gonadotrophs but not in thyrotrophs or placenta. The pattern of estrogen regulation observed in transgenic mice matched neither the repression that has been observed with human and bovine alpha-subunit promoters in transgenic mice nor the stimulation in mRNA levels reported in mares, suggesting a unique mechanism that is not recapitulated in the transgenic model. Thus the equine alpha-subunit promoter uses a combination of conserved and unique features of gene regulation to direct its pattern of tissue-specific expression.
authors.affiliation: Department of Pharmacology, Case Western Reserve University, Cleveland, Ohio 44106, USA.
authors.forename: T A
authors.initials: TA
authors.lastname: Farmerie
authors.forename: R A
authors.initials: RA
authors.lastname: Abbud
authors.forename: P R
authors.initials: PR
authors.lastname: Budworth
authors.forename: C M
authors.initials: CM
authors.lastname: Clay
authors.forename: R A
authors.initials: RA
authors.lastname: Keri
authors.forename: K J
authors.initials: KJ
authors.lastname: McDowell
authors.forename: M W
authors.initials: MW
authors.lastname: Wolfe
authors.forename: J H
authors.initials: JH
authors.lastname: Nilson
mesh_terms: D000818:Animals; D001483:Base Sequence; D015139:Blotting, Southern; D003001:Cloning, Molecular; D017931:DNA Primers; D015342:DNA Probes; D005260:Female; D015870:Gene Expression / Q000502:physiology*; D015723:Gene Library; D015292:Glycoprotein Hormones, alpha Subunit / Q000235:genetics*; D006736:Horses; D017403:In Situ Hybridization; D051379:Mice; D008822:Mice, Transgenic; D008969:Molecular Sequence Data; D010902:Pituitary Gland / Q000378:metabolism*; D010920:Placenta / Q000378:metabolism*; D010957:Plasmids; D016133:Polymerase Chain Reaction; D014162:Transfection
chemical_list: D017931:DNA Primers; D015342:DNA Probes; D015292:Glycoprotein Hormones, alpha Subunit