The in vivo effect of lipopolysaccharide on Na+,K(+)-ATPase catalytic (alpha) subunit isoforms in rat sciatic nerve.
The Na+,K(+)-ATPase catalytic (alpha) subunit in sciatic nerve of lipopolysaccharide (endotoxin, LPS)-treated rat was investigated. Using Western blot to determine subunit isoform polypeptide levels in rat sciatic nerve, we found a substantial reduction in alpha 1 polypeptide, but not that of alpha 2 and alpha 3 polypeptides, after the administration of LPS. Moreover, when rats were treated with polymyxin B (a LPS neutralizer) and NG-nitro-L-arginine (an inhibitor of nitric oxide (NO) synthase), the effects of LPS were reversed. These results implicate a specific marked deficit in alpha 1 subunit isoform of Na+,K(+)-ATPase in the pathogenesis of neuropathy during endotoxemia, through, at least in part, the L-arginine/NO pathway.
Liu
S H
SH
Institute of Toxicology, College of Medicine, National Taiwan University, Taipei. shliu@ntumc1.mc.ntu.edu.tw
Sheu
T J
TJ
D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology; D001665:Binding Sites; D002384:Catalysis; D019446:Endotoxemia / Q000201:enzymology*; D004791:Enzyme Inhibitors / Q000494:pharmacology; D007527:Isoenzymes / Q000187:drug effects*; D008070:Lipopolysaccharides / Q000494:pharmacology*; D008297:Male; D009569:Nitric Oxide / Q000502:physiology; D019335:Nitroarginine / Q000494:pharmacology; D010446:Peptide Fragments / Q000187:drug effects*; D011112:Polymyxin B / Q000494:pharmacology; D051381:Rats; D017208:Rats, Wistar; D012584:Sciatic Nerve / Q000187:drug effects* / Q000201:enzymology; D000254:Sodium-Potassium-Exchanging ATPase / Q000187:drug effects*
D000900:Anti-Bacterial Agents; D004791:Enzyme Inhibitors; D007527:Isoenzymes; D008070:Lipopolysaccharides; D010446:Peptide Fragments; D019335:Nitroarginine; D009569:Nitric Oxide; D000254:Sodium-Potassium-Exchanging ATPase; D011112:Polymyxin B