Expression profiles of transcripts from 126 open reading frames in the entire chromosome VI of Saccharomyces cerevisiae by systematic northern analyses.
Chromosome VI of Saccharomyces cerevisiae contains 126 open reading frames (ORFs), and the functions of proteins encoded by 80 ORFs are still unknown. In this report, we have systematically examined the expression profiles of all 126 ORFs on chromosome VI under five kinds of growth conditions by quantitative Northern hybridization. A series of Northern analyses and reverse transcription polymerase chain reactions have revealed that more than 64 novel ORFs are transcribed. Two ORFs (YFL059w and YFR011c) are specifically expressed in the presence of galactose. Two ORFs (YFL012w and YFR032c) are specifically transcribed in sporulation. Six ORFs (YFL049w, YFL035c, YFL010c, YFR006w, YFR010w and YFR017c) are abundantly expressed in many growth conditions.
Naitou
M
M
Cellular Physiology Laboratory, Tsukuba Life Science Center, Ibaraki, Japan.
Hagiwara
H
H
Hanaoka
F
F
Eki
T
T
Murakami
Y
Y
D015152:Blotting, Northern; D015825:Chromosomes, Fungal; D015870:Gene Expression; D005800:Genes, Fungal; D018869:Heat-Shock Response; D016366:Open Reading Frames; D012331:RNA, Fungal / Q000096:biosynthesis; D012333:RNA, Messenger / Q000096:biosynthesis; D012441:Saccharomyces cerevisiae / Q000235:genetics*; D013172:Spores, Fungal; D016615:Telomere / Q000235:genetics; D014158:Transcription, Genetic
D012331:RNA, Fungal; D012333:RNA, Messenger