T and B cell development in pituitary deficient insulin-like growth factor-II transgenic dwarf mice.
Treatment of mice with IGF-I stimulates T and B cell development. We showed that overexpression of IGF-II in transgenic FVB/N mice only stimulated T cell development. In the present study, we further addressed the in vivo effects of IGF-II in the absence of IGF-I to get more insight into the potential abilities of IGF-II to influence T and B cell development. To this end, we studied lymphocyte development in IGF-II transgenic Snell dwarf mice that are prolactin, GH and thyroid-stimulating hormone deficient and as a consequence show low serum IGF-I levels. We showed that T cell development was stimulated to the same extent as in IGF-II transgenic FVB/N mice. Furthermore, IGF-II increased the number of nucleated bone marrow cells and the number of immature B cells without having an effect on the number of mature B cells in spleen and bone marrow. Our data show that IGF-II has preferential effects on T cell development compared with B development, and that these preferential effects also occur in the absence of measurable IGF-I levels.
Kooijman
R
R
Department of Immunology, University Hospital for Children and Youth Het Wilhelmina Kinderziekenhuis, Utrecht, The Netherlands.
van Buul-Offers
S C
SC
Scholtens
L E
LE
Reijnen-Gresnigt
R G
RG
Zegers
B J
BJ
D000818:Animals; D001402:B-Lymphocytes / Q000502:physiology*; D001854:Bone Marrow Cells / Q000502:physiology; D017252:CD3 Complex / Q000276:immunology; D016516:CD4-CD8 Ratio; D002454:Cell Differentiation / Q000502:physiology; D004392:Dwarfism / Q000276:immunology*; D005260:Female; D005434:Flow Cytometry; D016130:Immunophenotyping; D007334:Insulin-Like Growth Factor I / Q000172:deficiency*; D007335:Insulin-Like Growth Factor II / Q000502:physiology*; D018655:Lymphocyte Count; D008297:Male; D051379:Mice; D008822:Mice, Transgenic; D013154:Spleen / Q000502:physiology; D013601:T-Lymphocytes / Q000502:physiology*
D017252:CD3 Complex; D007334:Insulin-Like Growth Factor I; D007335:Insulin-Like Growth Factor II