Immunoelectron microscopic localization of growth factors and other markers in human long-term bone marrow cultures.
Ultrastructural immunocytochemical characterization of human long-term bone marrow cultures has shown positive localization for growth factors on cell surface and on extracellular matrix (ECM). In some cases double-labelling indicated co-localization of growth factors and specific cell surface labels. Specific markers for endothelial cells and fibroblasts showed that growth factor (GM-CSF, G-CSF and b-FGF) were present at the surface of these cell types. Both scanning and transmissiorr electron microscopy indicated intense labelling for growth factors on the extracellular matrix. Double-labelling of heparan sulphate proteoglycans and GM-CSF showed a co-localization of the labelling, which indicated the binding of growth factor to the extracellular matrix.
Liu
J
J
Institute of Haemotology, CAMS, Tianjin.
de Wynter
E
E
Testa
N G
NG
Dexter
T M
TM
Allen
T D
TD
D001854:Bone Marrow Cells / Q000737:chemistry*; D002462:Cell Membrane / Q000737:chemistry; D002478:Cells, Cultured; D005109:Extracellular Matrix / Q000737:chemistry; D016222:Fibroblast Growth Factor 2 / Q000032:analysis*; D016179:Granulocyte Colony-Stimulating Factor / Q000032:analysis*; D016178:Granulocyte-Macrophage Colony-Stimulating Factor / Q000032:analysis*; D006801:Humans; D007377:Interleukin-3 / Q000032:analysis; D016253:Microscopy, Immunoelectron
D007377:Interleukin-3; D016222:Fibroblast Growth Factor 2; D016179:Granulocyte Colony-Stimulating Factor; D016178:Granulocyte-Macrophage Colony-Stimulating Factor