title: Heme peroxidase activity measured in single mosquitoes identifies individuals expressing an elevated oxidase for insecticide resistance.
abstract: Optimum conditions are described for a simple, rapid, microplate-based assay that indirectly measures the differences in oxidase levels between individual susceptible, resistant, or induced mosquitoes. A small proportion (0.01-0.1) of a single mosquito is used, allowing multiple replicates of the oxidase assay. Cytochrome C is used as a positive control. The levels of oxidase found in sample populations of pyrethroid-susceptible, pyrethroid-resistant, and phenobarbital-induced Anopheles albimanus mosquitoes are characterized with the assay.
authors.affiliation: Entomology Branch F-22, Centers for Disease Control and Prevention, Chamblee, GA 30341, USA.
authors.forename: W G
authors.initials: WG
authors.lastname: Brogdon
authors.forename: J C
authors.initials: JC
authors.lastname: McAllister
authors.forename: J
authors.initials: J
authors.lastname: Vulule
mesh_terms: D000818:Animals; D000852:Anopheles / Q000201:enzymology*; D003578:Cytochrome-c Peroxidase / Q000032:analysis; D005260:Female; D007305:Insecticide Resistance; D010088:Oxidoreductases / Q000032:analysis; D009195:Peroxidase / Q000032:analysis*; D011153:Population
chemical_list: D010088:Oxidoreductases; D003578:Cytochrome-c Peroxidase; D009195:Peroxidase