Expression of the Streptomyces aureofaciens glyceraldehyde-3-phosphate dehydrogenase gene (gap) is developmentally regulated and induced by glucose.
In previous experiments, the Streptomyces aureofaciens gap gene encoding glyceraldehyde-3-phosphate dehydrogenase (GAPDH) was identified. To investigate expression of the gene, S1 nuclease mapping and Northern blot hybridization were performed using RNA prepared from S. aureofaciens cultivated under various conditions. These studies suggested monocistronic organization and developmental regulation of the gene. A single promoter, gap-P, was identified upstream of the gap coding region. In cultures grown on solid medium in the absence of glucose, its transcription was induced at the time of aerial mycelium formation. In addition, gap transcription was also induced in substrate mycelium by glucose. A promoter-bearing DNA fragment was inserted into two promoter-probe vectors, to give expression patterns consistent with the results of direct RNA analysis.
Kormanec
J
J
Institute of Molecular Biology, Slovak Academy of Sciences, 842 51 Bratislava, Slovak Republic.
Lempel'ov
A
A
Institute of Molecular Biology, Slovak Academy of Sciences, 842 51 Bratislava, Slovak Republic.
Nov Kov
R
R
Institute of Molecular Biology, Slovak Academy of Sciences, 842 51 Bratislava, Slovak Republic.
Re Uchov
B
B
Institute of Molecular Biology, Slovak Academy of Sciences, 842 51 Bratislava, Slovak Republic.
Hom Rov
D
D
Institute of Molecular Biology, Slovak Academy of Sciences, 842 51 Bratislava, Slovak Republic.
D000595:Amino Acid Sequence; D001483:Base Sequence; D004790:Enzyme Induction; D015964:Gene Expression Regulation, Bacterial / Q000187:drug effects / Q000235:genetics*; D005798:Genes, Bacterial / Q000235:genetics / Q000502:physiology; D005947:Glucose / Q000494:pharmacology*; D005987:Glyceraldehyde-3-Phosphate Dehydrogenases / Q000096:biosynthesis / Q000235:genetics*; D008969:Molecular Sequence Data; D011401:Promoter Regions, Genetic / Q000235:genetics*; D012329:RNA, Bacterial / Q000032:analysis; D012333:RNA, Messenger / Q000032:analysis; D011993:Recombinant Fusion Proteins; D015183:Restriction Mapping; D013304:Streptomyces aureofaciens / Q000201:enzymology / Q000235:genetics*
D012329:RNA, Bacterial; D012333:RNA, Messenger; D011993:Recombinant Fusion Proteins; D005987:Glyceraldehyde-3-Phosphate Dehydrogenases; D005947:Glucose