A novel truncated variant form of Ebk/MDK1 receptor tyrosine kinase is expressed in embryonic mouse brain.
We have isolated cDNA clones from a mouse embryonic head cDNA library that encode one member of the Eph/Eck family of receptor tyrosine kinases (RTKs), Ebk/MDK1. Among the 10 clones, two showed full-length type comprising extracellular, transmembrane and intracellular kinase domains. Two of them were modified just after the transmembrane domain and stop codon appeared before completing the kinase domain. This truncated form also had a deletion of five amino acids at the extracellular domain, indicating that it is a novel variant of Ebk/MDK1. RNase protection assay showed that this truncated deleted type, named Ebk-td1, is present in the head of embryos, although the amount is less compared to that of the full-length type having a deletion of four amino acids. Considering the source and expression of Ebk/MDK1 mRNAs, they may have an important role, accompanied with a possible regulatory role of the truncated variant, during neural development and/or in embryogenesis.
Talukder
A H
AH
Department of Biochemistry, Nagoya University School of Medicine, Japan.
Muramatsu
T
T
Kaneda
N
N
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D001921:Brain / Q000196:embryology / Q000378:metabolism*; D001923:Brain Chemistry / Q000235:genetics; D018076:DNA, Complementary; D015870:Gene Expression; D015723:Gene Library; D051379:Mice; D008807:Mice, Inbred BALB C; D008969:Molecular Sequence Data; D020794:Receptor Protein-Tyrosine Kinases / Q000096:biosynthesis / Q000235:genetics*; D017386:Sequence Homology, Amino Acid
D018076:DNA, Complementary; D020794:Receptor Protein-Tyrosine Kinases