[Change of tumor necrosis factor and interleukin-6 in brochoalveolar lavage fluid of allergic asthma].
OBJECTIVE
To explain the change of TNF-alpha, IL-6 biological activity in supernatant of cultured AM and in BALF in the athmatic animal.


METHODS
The guinea pig model of asthma was established by frestly immunizing and then challenging the animal with 1% ovalbumin; The level of TNF-alpha and IL-6 were measured by L929 cytotoxic test and by the MH60. BSF2 cell proliferation test.


RESULTS
TNF-alpha and IL-6 in BALF and supernatant of AM in asthmatic animals were increased significantly, and there was a strong correlation between TNF-alpha and IL-6.


CONCLUSIONS
TNF-alpha and IL-6 might play a key role in the allergic airway inflamination and AM might be the source of TNF-alpha and IL-6.
Wang
W
W
Respiratory Department, Xijing Hospital, Fouth Military Medical University, Xian.
Chen
W
W
Sun
B
B
D000818:Animals; D001249:Asthma / Q000276:immunology / Q000378:metabolism*; D001992:Bronchoalveolar Lavage Fluid / Q000737:chemistry*; D006168:Guinea Pigs; D015850:Interleukin-6 / Q000378:metabolism*; D016676:Macrophages, Alveolar / Q000378:metabolism; D008297:Male; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism*
D015850:Interleukin-6; D014409:Tumor Necrosis Factor-alpha