Establishment and characterization of a new synovial sarcoma cell line, SN-SY-1: special reference to bcl-2 protein and SYT-SSX1 hybrid transcripts.
We established and characterized a new human synovial sarcoma cell line, SN-SY-1, derived from a monophasic fibrous synovial sarcoma excised from the abdominal wall of a 21-year-old man. We maintained the cell line for over 35 passages in vitro. The SN-SY-1 cells in vitro exhibit spindle and polygonal shapes and join to form an epithelial plaque. SN-SY-1 maintained a consistent karyotype: 46,t(X;18)(p11.2;q11.2), Y, t(6;8) (q13-15;q11-13), the same as that of the primary tumor specimen. RT-PCR assay for chimeric SYT-SSX transcripts followed by digestion with restriction enzymes revealed SYT-SSX1 fusion protein. An immunohistochemical study revealed expression of bcl-2 protein in the SN-SY-1 cell line, in vitro and in vivo. However, DNA analysis showed no rearrangement of the bcl-2 gene, and expression of bcl-2 mRNA was low. Why bcl-2 protein was expressed in this synovial sarcoma was not clarified.
Noguchi
S I
SI
Second Department of Pathology, Faculty of Medicine, Kyushu University, Fukuoka, Japan.
Ueki
T
T
Kawauchi
S
S
Fukuda
T
T
Matsuura
H
H
Sonoda
T
T
Tsuneyoshi
M
M
D000008:Abdominal Neoplasms / Q000235:genetics / Q000378:metabolism / Q000473:pathology / Q000601:surgery; D000328:Adult; D018929:Cell Culture Techniques / Q000379:methods; D002455:Cell Division; D002460:Cell Line; D002648:Child; D002874:Chromosome Mapping; D002887:Chromosomes, Human, Pair 18; D005260:Female; D019254:Genes, bcl-2; D006801:Humans; D007621:Karyotyping; D008297:Male; D008875:Middle Aged; D009363:Neoplasm Proteins; D016133:Polymerase Chain Reaction; D014176:Protein Biosynthesis; D055503:Protein Multimerization; D011506:Proteins; D011518:Proto-Oncogene Proteins; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000096:biosynthesis*; D012333:RNA, Messenger / Q000096:biosynthesis; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis; D012097:Repressor Proteins / Q000096:biosynthesis*; D013584:Sarcoma, Synovial / Q000235:genetics / Q000378:metabolism / Q000473:pathology* / Q000601:surgery; D014158:Transcription, Genetic; D014178:Translocation, Genetic; D014407:Tumor Cells, Cultured; D014960:X Chromosome
D009363:Neoplasm Proteins; D011506:Proteins; D011518:Proto-Oncogene Proteins; D019253:Proto-Oncogene Proteins c-bcl-2; D012333:RNA, Messenger; D011993:Recombinant Fusion Proteins; D012097:Repressor Proteins; C093961:SS18 protein, human; C093962:synovial sarcoma X breakpoint proteins