title: Preliminary structural studies on the multi-ligand-binding domain of the transcription activator, BmrR, from Bacillus subtilis.
abstract: In the bacterium Bacillus subtilis, the DNA-binding regulatory protein, BmrR, activates transcription from the multidrug transporter gene, bmr, after binding either rhodamine or tetraphenylphosphonium. These two compounds, which have no structural similarity, are also substrates for the bacterial multidrug transporter. BmrR belongs to the MerR family of transcription activators but differs from the other family members in its ability to bind unrelated small molecule activators. As an initial step in the elucidation of the mechanism by which BmrR recognizes rhodamine and tetraphenylphosphonium and activates transcription, we have crystallized the 144-amino acid-residue carboxy terminal dimerization/ligand-binding domain of the BmrR, named the BRC (BmrR C-terminus). Tetragonal crystals of ligand-free BRC take the space group P4(1)2(1)2, or its enantiomorph P4(3)2(1)2, with unit cell dimensions a = b = 76.3 A, c = 96.0 A, alpha = beta = gamma = 90 degrees. Diffraction is observed to at least 2.7 A resolution at room temperature. In addition, we determined the secondary structure content of ligand-free and rhodamine-bound BRC by circular dichroism. In the ligand-free form, BRC has considerable beta-sheet content (41%) and little alpha-helix structure (13%). After BRC binds rhodamine, its beta-sheet content increases to 47% while the alpha-helix structure decreases to 11%. The structure of BRC will provide insight not only into its multidrug recognition mechanism but could as well aid in the elucidation of the recognition and efflux mechanisms of Bmr and other bacterial multidrug transporters.
authors.affiliation: Department of Biochemistry and Molecular Biology, Oregon Health Sciences University, Portland 97201-3098, USA.
authors.forename: E E
authors.initials: EE
authors.lastname: Zheleznova
authors.forename: P N
authors.initials: PN
authors.lastname: Markham
authors.forename: A A
authors.initials: AA
authors.lastname: Neyfakh
authors.forename: R G
authors.initials: RG
authors.lastname: Brennan
mesh_terms: D001412:Bacillus subtilis; D001426:Bacterial Proteins / Q000737:chemistry*; D018360:Crystallography, X-Ray; D004268:DNA-Binding Proteins / Q000737:chemistry*; D008024:Ligands; D009861:Onium Compounds; D009943:Organophosphorus Compounds; D010446:Peptide Fragments / Q000737:chemistry; D012235:Rhodamines; D015534:Trans-Activators / Q000737:chemistry*
chemical_list: D001426:Bacterial Proteins; C098722:BmrR protein, bacteria; D004268:DNA-Binding Proteins; D008024:Ligands; D009861:Onium Compounds; D009943:Organophosphorus Compounds; D010446:Peptide Fragments; D012235:Rhodamines; D015534:Trans-Activators; C013289:tetraphenylphosphonium