Assessing enzyme substrate specificity using combinatorial libraries and electrospray ionization-Fourier transform ion cyclotron resonance mass spectrometry.
A model experiment for the 'on-line' screening of substrate libraries by enzymes using combinatorial libraries in combination with electrospray ionization-Fourier transform ion cyclotron resonance (ESI-FTICR) mass spectrometry has been performed. The reaction between the electrophilic substrate 1-chloro-2,4-dinitrobenzene and component of a H-gamma-Glu-Cys-Xxx-OH library, catalyzed by glutathione-S-transferase, has been monitored. It shows the feasibility of 'two-dimensional' screening of substrate libraries by ESI-FTICR mass spectrometry.
Wigger
M
M
Department of Chemistry, University of Florida, Gainesville 32611-7200, USA.
Nawrocki
J P
JP
Watson
C H
CH
Eyler
J R
JR
Benner
S A
SA
D017357:Cyclotrons; D004137:Dinitrochlorobenzene; D005583:Fourier Analysis; D005982:Glutathione Transferase / Q000378:metabolism; D007990:Libraries; D013058:Mass Spectrometry; D013379:Substrate Specificity
D004137:Dinitrochlorobenzene; D005982:Glutathione Transferase