title: Direct ligation of human CD4 polymerase chain reaction fragment into vectors at specific restriction sites with positional heterostagger cloning.
authors.affiliation: Biological Research Centre, Hungarian Academy of Sciences, Szeged, Hungary.
authors.forename: F
authors.initials: F
authors.lastname: Felfoldi
authors.forename: Z
authors.initials: Z
authors.lastname: Kupihar
authors.forename: S
authors.initials: S
authors.lastname: Bottka
authors.forename: L G
authors.initials: LG
authors.lastname: Puskas
mesh_terms: D015704:CD4 Antigens / Q000235:genetics*; D003001:Cloning, Molecular / Q000379:methods*; D004262:DNA Restriction Enzymes / Q000378:metabolism*; D004259:DNA-Directed DNA Polymerase / Q000378:metabolism; D005822:Genetic Vectors / Q000378:metabolism*; D006801:Humans; D008958:Models, Molecular; D016133:Polymerase Chain Reaction / Q000379:methods*; D019914:Taq Polymerase / Q000378:metabolism
chemical_list: D015704:CD4 Antigens; C072146:Pfu DNA polymerase; D019914:Taq Polymerase; D004259:DNA-Directed DNA Polymerase; D004262:DNA Restriction Enzymes