The role of tissue-fixed macrophages in apoptosis in the developing kidney.
Programmed cell death (PCD) and cell type involving dying cells in the developing mouse and human fetal kidneys were investigated using the modified TdT-mediated dUTP-biotin nick end-labeling (TUNEL) method in combination with immunohistochemistry. We identified many TUNEL-positive apoptotic cells mainly in the nephrogenic region in the developing mouse and human fetal kidneys. Tissue-fixed macrophages were widely distributed throughout the developing mouse and human fetal kidneys, including the nephrogenic zone. Most of the apoptotic cells in the nephrogenic zone were phagocytosed by tissue-fixed macrophages, suggesting that tissue-fixed macrophages play an important role in the process of PCD in the developing mouse kidney and human fetal kidney.
Yasui
M
M
Department of Pediatrics, Okayama University Medical School, Japan.
Tanaka
H
H
Seino
Y
Y
D000818:Animals; D000911:Antibodies, Monoclonal; D015703:Antigens, CD / Q000032:analysis; D015214:Antigens, Differentiation, Myelomonocytic / Q000032:analysis; D017209:Apoptosis / Q000502:physiology*; D018845:CD11 Antigens / Q000032:analysis; D053938:DNA Fragmentation; D006801:Humans; D007150:Immunohistochemistry; D007668:Kidney / Q000166:cytology / Q000196:embryology / Q000254:growth & development*; D008264:Macrophages / Q000276:immunology / Q000502:physiology*; D051379:Mice; D008807:Mice, Inbred BALB C; D008813:Mice, Inbred ICR; D008856:Microscopy, Fluorescence; D010587:Phagocytosis / Q000502:physiology; D011419:Propidium; D000097382:CD68 Molecule
D000911:Antibodies, Monoclonal; D015703:Antigens, CD; D015214:Antigens, Differentiation, Myelomonocytic; D018845:CD11 Antigens; D011419:Propidium; C067980:CD68 antigen, human; D000097382:CD68 Molecule