[An analysis of the blood sera of patients infected with HIV-1 for the presence of antibodies to the gp120 and gp41 sequences].
Blood sera, originating four regions of Russia and Byelorussia and previously tested for the content of antibodies to HIV-1 proteins, were studied in the enzyme immunoassay on the basis of recombinant sequences gp160, as well as on the basis of oligopeptides corresponding to sequences V3 of six HIV-1 strains. The possibility of using sequences gp160, contained in fusion polypeptides synthesized in Escherichia coli cells, for the detection of antibodies in laboratory research was shown. Differences in the reactivity of the sera under study with respect to fragments gp160 correlated with the geographical origin of these sera: similarity between the serum samples from Elista and Rostov and their difference from serum samples collected in other regions were shown.
Sergeev
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Garaev
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D000595:Amino Acid Sequence; D004926:Escherichia coli / Q000276:immunology; D015483:HIV Antibodies / Q000097:blood*; D015699:HIV Envelope Protein gp120 / Q000276:immunology*; D019302:HIV Envelope Protein gp160 / Q000276:immunology; D015700:HIV Envelope Protein gp41 / Q000276:immunology*; D015658:HIV Infections / Q000175:diagnosis*; D015497:HIV-1 / Q000276:immunology*; D006801:Humans; D007124:Immunoenzyme Techniques; D008969:Molecular Sequence Data; D010446:Peptide Fragments / Q000276:immunology; D011994:Recombinant Proteins / Q000276:immunology; D002094:Republic of Belarus; D012426:Russia
D015483:HIV Antibodies; D015699:HIV Envelope Protein gp120; D019302:HIV Envelope Protein gp160; D015700:HIV Envelope Protein gp41; C059762:HIV envelope protein gp120 (305-321); D010446:Peptide Fragments; D011994:Recombinant Proteins