title: [The cloning and expression of salmon calcitonin gene in Escherichia coli].
abstract: The DNA fragment encoding salmon calcitonin was separated from the salmon DNA with PCR method. It was recombined into the fusion expression vector (pGEX-2T) which included a Glutathion-S-Transferase (GST) gene and was transformed into E. coli JM109 and then expression was induced with IPTG. The fusion protein (GST-sCT) accounted for 38%-40% of the total cellular protein and was purified from the soluble expression products by glutathion sepharose 4B affinity chromatography. The purity of GST-sCT is about 80% and it showed a positive immunological reaction in calcitonin enzyme immunoassay.
authors.affiliation: Institute of Basic Medical Sciences, CAMS, Beijing.
authors.forename: X
authors.initials: X
authors.lastname: Di
authors.forename: S
authors.initials: S
authors.lastname: Chen
authors.forename: F
authors.initials: F
authors.lastname: Song
authors.forename: J
authors.initials: J
authors.lastname: Zhang
authors.forename: W
authors.initials: W
authors.lastname: Chen
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D002116:Calcitonin / Q000096:biosynthesis* / Q000235:genetics; D003001:Cloning, Molecular; D004247:DNA / Q000235:genetics; D004926:Escherichia coli / Q000235:genetics*; D015870:Gene Expression; D005982:Glutathione Transferase / Q000096:biosynthesis* / Q000235:genetics; D008969:Molecular Sequence Data; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis* / Q000235:genetics; D014170:Transformation, Genetic
chemical_list: D011993:Recombinant Fusion Proteins; C028815:salmon calcitonin; D002116:Calcitonin; D004247:DNA; D005982:Glutathione Transferase