Galactosylation with beta-galactosidase from bovine testes employing modified acceptor substrates.
In this study beta1-3 linked analogues of the T-antigen determinant were synthesized in preparative scale by transgalactosylation using beta-galactosidase from bovine testes to give synthetic antigens. Acceptors with modifications of the sugar residue such as alpha-glycosylated spacers, as well as GlcNAc-alphaOR- and 2dGal-alphaOR-substrates opened further possibilities for galactosylation.
Gambert
U
U
Institut für Organische Chemie, Universität Hamburg, Germany.
Gonzalez Lio
R
R
Farkas
E
E
Thiem
J
J
Verez Bencomo
V
V
Lipták
A
A
D000818:Animals; D000957:Antigens, Viral, Tumor / Q000737:chemistry*; D002417:Cattle; D004187:Disaccharides / Q000138:chemical synthesis*; D000939:Epitopes / Q000737:chemistry; D005697:Galactosides / Q000138:chemical synthesis*; D006031:Glycosylation; D008297:Male; D013379:Substrate Specificity; D013737:Testis / Q000201:enzymology*; D001616:beta-Galactosidase / Q000378:metabolism*
D000957:Antigens, Viral, Tumor; D004187:Disaccharides; D000939:Epitopes; D005697:Galactosides; D001616:beta-Galactosidase