Recombinant human transforming growth factor beta 3 accelerates gastric ulcer healing in rats.
BACKGROUND
Gastric ulcer healing is mediated by various endogenous growth factors. In this experimental study effect of locally and systemically applied recombinant human transforming growth factor beta 3 (rhTGF-beta 3) on gastric ulcer healing was investigated in the rat.


RESULTS
Gastric ulcers were induced with a cryoprobe, and ulcer healing was evaluated 7 days after local infiltration (0.5 micrograms, 1.0 microgram, 2.5 micrograms, and 50 micrograms) or systemic (intravenous) application of TGF-beta 3 (500 micrograms/kg body weight). Compared with controls, a dose-dependent stimulation of ulcer healing (as evidenced by a reduction in ulcer size) was observed 7 days after local infiltration of TGF-beta 3 (1.0 microgram, 2.5 micrograms, and 50 micrograms). Corresponding increases in the levels of proliferating cell nuclear antigen (PCNA) and intracellular TGF-beta 3 expression and a downregulation of the TGF-beta type-II receptor expression were also observed in the granulation tissue of the ulcer margins. Systemic application of TGF-beta 3 produced effects similar to those observed after local treatment with 50 micrograms of the compound.


CONCLUSIONS
Local and systemic TGF-beta 3 treatment accelerates gastric ulcer healing in rats.
Coerper
S
S
Dept. of General and Transplant Surgery, University of Tübingen Germany.
Sigloch
E
E
Cox
D
D
Starlinger
M
M
Köveker
G
G
Becker
H D
HD
D000818:Animals; D004305:Dose-Response Relationship, Drug; D005260:Female; D006801:Humans; D051381:Rats; D017208:Rats, Wistar; D011994:Recombinant Proteins / Q000627:therapeutic use; D013268:Stimulation, Chemical; D013276:Stomach Ulcer / Q000188:drug therapy*; D013997:Time Factors; D016212:Transforming Growth Factor beta / Q000627:therapeutic use*; D014945:Wound Healing / Q000187:drug effects*
D011994:Recombinant Proteins; D016212:Transforming Growth Factor beta