title: Haemodialyser biocompatibility and erythrocyte structure and function.
abstract: There is controversy as to the clinical importance of providing haemodialysis (HD) with biocompatible versus non-biocompatible membranes. The effects of both acute and chronic dialysis with a biocompatible membrane (polyacrylonitrile, PAN) and a non-biocompatible membrane (cuprophane, CU) on the structural and functional properties of human erythrocytes have been examined. All 27 studied HD patients had increased erythrocyte osmotic fragility (OF) compared to controls; a single CU HD decreased mean OF (% lysis) by 13% without altering cell cholesterol. A single PAN HD decreased OF by a significantly greater amount (24%) and was associated with a 20% reduction in cell cholesterol. Chronic PAN HD for 6 months was associated with a sustained reduction in osmotic fragility compared to chronic CU HD (mean lysis 16% vs 45%) with no differences in mean pre-HD cell cholesterol. A single CU HD was associated with increased mean erythrocyte malonyldialdehyde (MDA) and reduced membrane content of spectrin and band 3 and this was significantly different from the effects of PAN. A single CU or PAN HD had no significant action on reduced glutathione (GSH), ankyrin, actin or sodium pump activity. Chronic HD was associated with increased GSH, and decreased ankyrin and band 3 protein compared with controls but the results for CU and PAN were not different. There was a non-significant tendency for higher MDA levels after chronic CU HD compared to PAN. These results indicate that the structural integrity of erythrocytes is improved by PAN HD with respect to CU but this difference cannot easily be ascribed to gross changes in structural proteins, ionic homeostasis or oxidation status.
authors.affiliation: Department of Physiology, University of Alcalá de Henares, Spain.
authors.forename: M R
authors.initials: MR
authors.lastname: Martos
authors.forename: B M
authors.initials: BM
authors.lastname: Hendry
authors.forename: M
authors.initials: M
authors.lastname: Rodríguez-Puyol
authors.forename: J
authors.initials: J
authors.lastname: Dwight
authors.forename: M L
authors.initials: ML
authors.lastname: Díez-Marqués
authors.forename: D
authors.initials: D
authors.lastname: Rodríguez-Puyol
mesh_terms: D000180:Acrylic Resins / Q000494:pharmacology*; D000328:Adult; D001672:Biocompatible Materials / Q000494:pharmacology*; D002482:Cellulose / Q000031:analogs & derivatives* / Q000494:pharmacology; D002784:Cholesterol / Q000097:blood; D004910:Erythrocyte Membrane / Q000378:metabolism; D004912:Erythrocytes / Q000166:cytology* / Q000378:metabolism / Q000502:physiology*; D005260:Female; D005978:Glutathione / Q000097:blood; D006801:Humans; D008297:Male; D008315:Malondialdehyde / Q000097:blood; D008565:Membrane Proteins / Q000097:blood; D008567:Membranes, Artificial; D008875:Middle Aged; D009996:Osmotic Fragility; D010042:Ouabain / Q000494:pharmacology; D006435:Renal Dialysis / Q000295:instrumentation*; D012680:Sensitivity and Specificity; D000254:Sodium-Potassium-Exchanging ATPase / Q000097:blood
chemical_list: D000180:Acrylic Resins; D001672:Biocompatible Materials; D008565:Membrane Proteins; D008567:Membranes, Artificial; C010504:polyacrylonitrile; D008315:Malondialdehyde; D010042:Ouabain; D002482:Cellulose; C009865:cuprammonium cellulose; D002784:Cholesterol; D000254:Sodium-Potassium-Exchanging ATPase; D005978:Glutathione