[Chemosensitivity test for human osteosarcoma cells].
In this experiment, the sensitivity of human osteosarcoma cells to various concentration gradients of HDMTX, VCR, CBP, MMC, VP16, PMB and their time-effect relationship were examined in vitro with MTT assay among 23 cases' fresh osteosarcoma (OS) tissues. It was found that OS was sensitive to MMC and PMB when the drug concentrations were equal to the calculated in vivo drug concentrations. When the concentrations were 5 times as high as those of the calculated in vivo concentrations, OS was sensitive to HDMTX, CBP, MMC and PMB. The positive rates of sensitivity were highest in 72 hours with an average of 55.1%.
Liu
C
C
Institute of Orthopaedics, Second Teaching Hospital, Lanzhou Medical College.
Dai
G
G
Zhang
X
X
D000903:Antibiotics, Antineoplastic / Q000494:pharmacology*; D001859:Bone Neoplasms / Q000473:pathology*; D004354:Drug Screening Assays, Antitumor; D015251:Epirubicin / Q000494:pharmacology*; D006801:Humans; D016685:Mitomycin / Q000494:pharmacology*; D012516:Osteosarcoma / Q000473:pathology*; D014407:Tumor Cells, Cultured
D000903:Antibiotics, Antineoplastic; D015251:Epirubicin; D016685:Mitomycin