title: Micellar electrokinetic capillary chromatography combined with immunoaffinity chromatography for identification and determination of dexamethasone and flumethasone in equine urine.
abstract: A capillary electrophoresis technique was developed for the separation of synthetic glucocorticoids and the determination of dexamethasone and flumethasone in horse urine. Pretreatment of the sample using a dexamethasone affinity column resulted in low background that enabled the authors to detect levels as low as 1.1 ng/mL and 2.7 ng/mL for dexamethasone and flumethasone in horse urine, respectively. The developed method was used to detect dexamethasone in horse urine samples after the injection of a therapeutic dose of dexamethasone for up to 12 hr postinjection. The optimum conditions for capillary electrophoresis and dexamethasone elution from the affinity column are described.
authors.affiliation: Department of Large Animal Clinical Sciences, College of Veterinary Medicine, University of Florida, Gainesville 32610-0136, USA.
authors.forename: X
authors.initials: X
authors.lastname: Gu
authors.forename: M
authors.initials: M
authors.lastname: Meleka-Boules
authors.forename: C L
authors.initials: CL
authors.lastname: Chen
mesh_terms: D000818:Animals; D000906:Antibodies; D002197:Capillary Action; D002845:Chromatography / Q000295:instrumentation / Q000379:methods; D002846:Chromatography, Affinity / Q000295:instrumentation / Q000379:methods; D002851:Chromatography, High Pressure Liquid; D003907:Dexamethasone / Q000302:isolation & purification / Q000493:pharmacokinetics / Q000652:urine*; D019075:Electrophoresis, Capillary / Q000295:instrumentation / Q000379:methods; D005260:Female; D005443:Flumethasone / Q000302:isolation & purification / Q000652:urine*; D005938:Glucocorticoids / Q000302:isolation & purification / Q000652:urine*; D006736:Horses; D007074:Immunoglobulin G; D008823:Micelles; D011817:Rabbits; D011863:Radioimmunoassay; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
chemical_list: D000906:Antibodies; D005938:Glucocorticoids; D007074:Immunoglobulin G; D008823:Micelles; D003907:Dexamethasone; D005443:Flumethasone