High-resolution physical map of the immunoglobulin lambda variant gene cluster assembled by quantitative DNA fiber mapping.
Quantitative DNA fiber mapping (QDFM) allows rapid construction of near-kilobase-resolution physical maps by hybridizing specific probes to individual stretched DNA molecules. We evaluated the utility of QDFM for the large-scale physical mapping of a rather unstable, repeat-rich 850-kb region encompassing the immunoglobulin lambda variant (IGLV) gene segments. We mapped a minimal tiling path composed of 32 cosmid clones to three partially overlapping yeast artificial chromosome (YAC) clones and determined the physical size of each clone, the extent of overlap between clones, and contig orientation, as well as the sizes of gaps between adjacent contigs. Regions of germline DNA for which we had no YAC coverage were characterized by cosmid to cosmid hybridizations. Compared to other methods commonly used for physical map assembly, QDFM is a rapid, versatile technique delivering unambiguous data necessary for map closure and preparation of sequence-ready minimal tiling paths.
Duell
T
T
Life Sciences Division, University of California, Berkeley, California, 94720, USA.
Wang
M
M
Wu
J
J
Kim
U J
UJ
Weier
H U
HU
D002874:Chromosome Mapping / Q000379:methods*; D018244:Chromosomes, Artificial, Yeast; D002892:Chromosomes, Human, Pair 22; D003360:Cosmids / Q000235:genetics; D015342:DNA Probes; D006801:Humans; D007146:Immunoglobulin lambda-Chains / Q000235:genetics*; D017404:In Situ Hybridization, Fluorescence; D005810:Multigene Family; D012091:Repetitive Sequences, Nucleic Acid
D015342:DNA Probes; D007146:Immunoglobulin lambda-Chains