Use of high-performance liquid chromatography as an extraction procedure for analysis of triazolam in decomposed human muscle by gas chromatography-mass spectrometry.
A reliable and sensitive method for the determination of triazolam in human muscle using gas chromatography-mass spectrometry (GC-MS) is described. The drug was extracted from decomposed human muscle using three-step liquid-liquid extraction and HPLC which was performed isocratically on a conventional ODS column with a mobile phase of 0.01 M phosphate buffer (pH 6.5)-acetonitrile (7:3). Estazolam was used as an internal standard. GC-MS analysis was performed on a DB-5 capillary column. Excellent linearity was obtained over the concentration range 1-200 ng/g. The lower limit of detection was approximately 0.5 ng/g. Forensic application of the present method was also described.
Inoue
H
H
Department of Legal Medicine, Nagoya City University Medical School, Nagoya, Japan.
Maeno
Y
Y
Iwasa
M
M
Monma
J
J
Matoba
R
R
D002851:Chromatography, High Pressure Liquid; D005554:Forensic Medicine / Q000379:methods; D008401:Gas Chromatography-Mass Spectrometry; D006801:Humans; D006993:Hypnotics and Sedatives / Q000032:analysis*; D008297:Male; D018482:Muscle, Skeletal / Q000737:chemistry*; D011180:Postmortem Changes; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D014229:Triazolam / Q000032:analysis*
D006993:Hypnotics and Sedatives; D014229:Triazolam