title: A malignant astrocytoma containing simian virus 40 DNA in a macaque infected with simian immunodeficiency virus.
abstract: Polyomaviruses have proven oncogenicity in nonhost experimental animals; however, studies concerning the association between human brain tumors and simian and human polyomaviruses have yielded inconclusive results. We examined the relationship of SV40 to a malignant astrocytoma found in the right frontal lobe of a pigtail macaque (Macaca nemestrina) infected with simian immunodeficiency virus (SIV). Consistent with the histologic diagnosis, the tumor was immunoreactive with antibodies to S-100 protein, vimentin, and glial fibrillary acidic protein, but negative for neurofilament protein, synaptophysin, neuron-specific enolase, and chromogranin A. At the time of SIV inoculation, the animal was seropositive for SV40. Polymerase chain reaction assay of tumor DNA, but not normal brain DNA, yielded a 300 base-pair fragment corresponding to the carboxy-terminal coding region (C-terminus) of the large T antigen gene of SV40, suggesting an association with the tumor.
authors.affiliation: Division of Comparative Pathology, New England Regional Primate Research Center, Harvard Medical School, Southborough, Massachusetts 01772-9102, USA.
authors.forename: J P
authors.initials: JP
authors.lastname: Hurley
authors.forename: P O
authors.initials: PO
authors.lastname: Ilyinskii
authors.forename: C J
authors.initials: CJ
authors.lastname: Horvath
authors.forename: M A
authors.initials: MA
authors.lastname: Simon
mesh_terms: D000818:Animals; D001254:Astrocytoma / Q000235:genetics / Q000662:veterinary / Q000821:virology*; D001483:Base Sequence; D001932:Brain Neoplasms / Q000235:genetics / Q000662:veterinary / Q000821:virology*; D004279:DNA, Viral; D005625:Frontal Lobe; D006801:Humans; D007150:Immunohistochemistry; D017403:In Situ Hybridization; D008254:Macaca nemestrina / Q000235:genetics / Q000502:physiology / Q000821:virology; D008297:Male; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D016097:Simian Acquired Immunodeficiency Syndrome / Q000235:genetics / Q000821:virology; D015302:Simian Immunodeficiency Virus / Q000235:genetics*; D013539:Simian virus 40 / Q000235:genetics*
chemical_list: D004279:DNA, Viral