Enrichment of antigen-specific T lymphocytes by panning on immobilized MHC-peptide complexes.
Numerous studies have focused on characterizing and monitoring antigen-specific T cells during the course of an immune response. Mostly indirect methods were used to circumvent the low frequency of T cell precursors and the inherent complexity of T cell receptor (TcR)-MHC-peptide interactions. Here, we took advantage of peptide-specific adhesion induced by immobilized MHC-peptide complexes. We describe a simple technique which allows enrichment in antigen-specific T lymphocytes among a heterogeneous CD8+ T cell population. Enrichment of T cells according to their specificity should facilitate their characterization and provide an attractive tool for immunotherapy.
Bousso
P
P
Département d'immunologie, INSERM U277, Institut Pasteur, Paris, France.
Michel
F
F
Pardigon
N
N
Bercovici
N
N
Liblau
R
R
Kourilsky
P
P
Abastado
J P
JP
D000818:Animals; D016466:CHO Cells; D002448:Cell Adhesion; D018929:Cell Culture Techniques / Q000379:methods; D002469:Cell Separation / Q000379:methods*; D006224:Cricetinae; D019281:Dimerization; D008213:Lymphocyte Activation; D008285:Major Histocompatibility Complex; D051379:Mice; D008811:Mice, Inbred DBA; D010455:Peptides / Q000276:immunology*; D011948:Receptors, Antigen, T-Cell / Q000276:immunology; D013601:T-Lymphocytes / Q000166:cytology* / Q000276:immunology
D010455:Peptides; D011948:Receptors, Antigen, T-Cell