title: Paramagnetic NMR shifts in cyanoferricytochrome c. Investigation of thermal stability and deviations from Curie law behaviour.
abstract: The paramagnetic shifts of 13C nuclei positioned alpha to the haem in cyanoferricytochrome c are reported and analysed in terms of molecular orbitals based on D4h symmetry with a rhombic perturbation. The temperature dependence of the Fermi contact and dipolar shifts of the haem and axial histidine ligand show deviations from Curie Law behaviour which are explained by a Boltzmann distribution between partially filled 3e(pi) molecular orbitals and the ground and first excited state Kramers doublets. The comprehensive explanation of the temperature dependence of the paramagnetic shifts leads to the conclusion that there is no detectable temperature dependence of the haem orientation or that of the His ligand orientation. This work also provides evidence for the role of the axial His ligand in determining the orientation of the magnetic z-axis.
authors.affiliation: Department of Chemistry, University of Southampton, UK.
authors.forename: L
authors.initials: L
authors.lastname: Brennan
authors.forename: D L
authors.initials: DL
authors.lastname: Turner
mesh_terms: D003574:Cytochrome c Group / Q000737:chemistry*; D045304:Cytochromes c; D004355:Drug Stability; D004578:Electron Spin Resonance Spectroscopy; D006418:Heme; D007700:Kinetics; D008956:Models, Chemical; D019906:Nuclear Magnetic Resonance, Biomolecular / Q000379:methods; D013816:Thermodynamics
chemical_list: D003574:Cytochrome c Group; C109096:cyanoferricytochrome C; D006418:Heme; D045304:Cytochromes c