Effect of 3-amino-1-propanol on the phosphatidylinositol (PI) and glycosyl phosphatidylinositol (GPI) systems of Tetrahymena.
3-Amino-1-propanol (AP), a substance replacing ethanolamine in phosphatidylethanolamine (PE) significantly reduced 32P incorporation to phosphatidylinositol (PI) and glycosyl-phosphatidylinositol (GPI) in the unicellular organism Tetrahymena pyriformis. At 10 mM, AP completely inhibited the incorporation of 32P into PI. 3H-arachidonate incorporation into PI was also inhibited, while that into diacylglycerol (DAG) was high. The experiments indicate the presence and metabolism of inositol phospholipids and GPI in T. pyriformis.
Kovács
P
P
Department of Biology, Semmelweis University of Medicine, Budapest, Hungary.
Kõhidai
L
L
Csaba
G
G
D000818:Animals; D016718:Arachidonic Acid / Q000378:metabolism; D002478:Cells, Cultured; D017261:Glycosylphosphatidylinositols / Q000378:metabolism*; D050356:Lipid Metabolism; D010716:Phosphatidylinositols / Q000378:metabolism*; D010743:Phospholipids / Q000378:metabolism; D010761:Phosphorus Radioisotopes; D011412:Propanolamines / Q000494:pharmacology*; D013768:Tetrahymena / Q000187:drug effects* / Q000378:metabolism; D014316:Tritium
D017261:Glycosylphosphatidylinositols; D010716:Phosphatidylinositols; D010743:Phospholipids; D010761:Phosphorus Radioisotopes; D011412:Propanolamines; D014316:Tritium; C109550:3-amino-1-propanol; D016718:Arachidonic Acid