title: Molecular cloning of cyclic nucleotide-gated cation channel subunits from rat pineal gland.
abstract: We have investigated the subunit composition and the molecular structure of the CNG channel expressed in rat pineal gland. Three types of subunits have been cloned: an alpha-subunit (CNG1), two beta-subunit splice variants (rCNG4.1 and rCNG4.2) and a hydrophilic glutamic acid-rich protein (rGARP). In situ hybridization with sections of rat brain revealed the co-expression of CNG1, CNG4 and GARP in pinealocytes. In addition, CNG4-specific transcripts were detected in the arcuate, periventricular and supraoptic nuclei of the hypothalamus.
authors.affiliation: Institut für Pharmakologie und Toxikologie der Technischen Universität München, Germany.
authors.forename: A
authors.initials: A
authors.lastname: Sautter
authors.forename: M
authors.initials: M
authors.lastname: Biel
authors.forename: F
authors.initials: F
authors.lastname: Hofmann
mesh_terms: D017398:Alternative Splicing; D000595:Amino Acid Sequence; D000818:Animals; D001921:Brain / Q000378:metabolism*; D003001:Cloning, Molecular; D003062:Codon; D054815:Cyclic Nucleotide-Gated Cation Channels; D006801:Humans; D007473:Ion Channels / Q000096:biosynthesis* / Q000737:chemistry; D046911:Macromolecular Substances; D008565:Membrane Proteins / Q000096:biosynthesis* / Q000737:chemistry; D008969:Molecular Sequence Data; D009928:Organ Specificity; D010870:Pineal Gland / Q000378:metabolism*; D051381:Rats; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid; D014158:Transcription, Genetic
chemical_list: D003062:Codon; D054815:Cyclic Nucleotide-Gated Cation Channels; D007473:Ion Channels; C087215:LRRC32 protein, human; D046911:Macromolecular Substances; D008565:Membrane Proteins; C484197:rGarp protein, rat