Genomic organization of the yeast Yarrowia lipolytica.
We produced electrophoretic karyotypes of the reference strain E150 and of seven other isolates from different geographical origins to study the genomic organization of the dimorphic yeast Yarrowia lipolytica. These karyotypes differed in the number and size of the chromosomal bands. The karyotype of the reference stain E150 consisted of five bands of between 2.6 and 4.9 Mb in size. This strain contained at least five rDNA clusters, from 190 to 620 kb in size, which were scattered over most of the chromosomes. The assignment of 43 markers, including rRNA genes and three centromeres, to the E150 bands defined five linkage groups. Hybridization to the karyotypes of other isolates with pools of markers of each linkage group showed that linkage groups I, II, IV and V were conserved in the strains tested whereas group III was not and was split between at least two chromosomes in most strains. Use of a meganuclease I-SceI site targeted to one locus of E150 linkage group III showed that two chromosomes actually comigrated in band III of this strain. Our results are compatible with six chromosomes defining the haploid complement of strains of Y. lipolytica and that, despite an unprecedented chromosome length polymorphism, the overall structure of the genome is conserved in different isolates.
Casarégola
S
S
Collection de Levures d'Intérêt Biotechnologique (CLIB), Laboratoire de Génétique Moléculaire et Cellulaire, INRA/CNRS/INA-PG, F-78850 Thiverval-Grignon, France. serge@cardere.grignon.inra.fr
Feynerol
C
C
Diez
M
M
Fournier
P
P
Gaillardin
C
C
D001203:Ascomycota / Q000235:genetics*; D002874:Chromosome Mapping; D015825:Chromosomes, Fungal / Q000235:genetics; D004275:DNA, Ribosomal / Q000235:genetics; D016521:Electrophoresis, Gel, Pulsed-Field / Q000379:methods; D005800:Genes, Fungal / Q000235:genetics; D008040:Genetic Linkage; D016681:Genome, Fungal; D007621:Karyotyping; D008540:Meiosis / Q000235:genetics
D004275:DNA, Ribosomal