EGF inhibits expression of WDNM1 and sulfated glycoprotein-2 genes in mammary epithelial cells.
We have previously shown that expressions of ferritin heavy chain (FHC), WDNM1, and sulfated glycoprotein-2 (SGP-2) genes are induced at an involution stage of mammary gland. Here we studied the effect of lactogenic hormones and EGF on the expression of involution-induced genes in HC11 mammary epithelial cells. Insulin, dexamethasone, prolactin, and its combinations did not affect expression of the genes. When cells were cultured in growth medium containing EGF, expression of WDNM1 and SGP-2 genes was strongly inhibited in a dose- and time- dependent manner, whereas expression of FHC gene was not influenced by EGF. Results demonstrate that EGF inhibits expression of WDNM1 and SGP-2 genes in mammary epithelial cells.
Lee
M
M
Department of Genetic Engineering, Chonnam National University, Kwangju, Korea.
Hwang
I
I
Choi
Y
Y
Paik
S
S
Lee
H B
HB
Baik
M
M
D000818:Animals; D002460:Cell Line; D051152:Clusterin; D003907:Dexamethasone / Q000494:pharmacology; D004815:Epidermal Growth Factor / Q000494:pharmacology*; D004847:Epithelial Cells / Q000187:drug effects / Q000378:metabolism* / Q000502:physiology; D005260:Female; D005293:Ferritins / Q000235:genetics; D005786:Gene Expression Regulation / Q000187:drug effects*; D006023:Glycoproteins / Q000037:antagonists & inhibitors* / Q000235:genetics*; D007328:Insulin / Q000494:pharmacology; D007774:Lactation / Q000235:genetics; D008321:Mammary Glands, Animal / Q000187:drug effects / Q000378:metabolism* / Q000502:physiology; D051379:Mice; D018832:Molecular Chaperones; D009363:Neoplasm Proteins / Q000037:antagonists & inhibitors* / Q000235:genetics*; D011388:Prolactin / Q000494:pharmacology
C495243:Clu protein, mouse; D051152:Clusterin; D006023:Glycoproteins; D007328:Insulin; D018832:Molecular Chaperones; D009363:Neoplasm Proteins; C068304:Wfdc18 protein, rat; D004815:Epidermal Growth Factor; D003907:Dexamethasone; D011388:Prolactin; D005293:Ferritins