Clinical field site evaluation of the FACSCount for absolute CD3+, CD3+ CD4+, and CD3+ CD8+ cell count determinations in Thailand.
The FACSCount flow cytometer absolute CD3+, CD3+ CD4+, and CD3+ CD8+ cell counts measured at a field site hospital laboratory in Thailand were compared to FACScan absolute counts obtained at a nearby research laboratory. Correlation coefficients for 208 samples were > or = 0.95. The FACSCount was accurate, and it was easier and less expensive to operate than the FACScan. Additionally, the FACScan-generated lymphocyte percentage value was accurate for use with the FACScan SimulSET software.
Young
N L
NL
HIV/AIDS Collaboration and Department of Communicable Disease Control, Central Chest Hospital, Nonthaburi, Thailand. NLY0@cdc.gov
Ponglertnapakorn
P
P
Shaffer
N
N
Srisak
K
K
Chaowanachan
T
T
On-Thern
V
V
Kittinunvorakoon
C
C
Bunwattanakul
A
A
Suksaweang
S
S
Pobkeeree
V
V
Punnotok
J
J
Mastro
T D
TD
D017252:CD3 Complex / Q000032:analysis*; D015704:CD4 Antigens / Q000032:analysis*; D016827:CD8 Antigens / Q000032:analysis*; D005434:Flow Cytometry / Q000379:methods*; D015658:HIV Infections / Q000097:blood; D006679:HIV Seropositivity; D006801:Humans; D007958:Leukocyte Count / Q000379:methods*; D014376:Tuberculosis / Q000097:blood
D017252:CD3 Complex; D015704:CD4 Antigens; D016827:CD8 Antigens