Temperature effect on chiral recognition of some amino acids with molecularly imprinted polymer filled capillary electrochromatography.
L-Aromatic amino acid imprinted polymers were prepared using azobisnitriles as either photoinitiators or thermal initiators at temperature ranging from 4 to 60 degrees C. Methacrylic acid (MAA) was used as functional monomer and ethylene glycol dimethacrylate (EDMA) was used as cross-linker. The result polymers were ground and sieved to particles < or = 10 microns, filled into the capillary columns and used for enantiomeric separations in capillary electrochromatographic (CEC) mode. The polymer particles < or = 25 were packed into high performance liquid chromatographic (HPLC) columns and used for enantioseparations in the HPLC mode. The mobility and chiral separation of some amino acids were studied on CEC and HPLC columns at different temperature. The relationships of separation factor and column temperatures and demonstrated in linear between logarithm of the separation factor (in alpha) and the inverse of absolute temperature (1/T). Some thermodynamic parameters, such as the apparent change of enthalpy (delta H zero) and the apparent change of entropy (delta S zero), were obtained using van't Hoff plots. The information obtained from the thermodynamic study is discussed in developing strategies for chiral recognitions of amino acids by molecular imprinting technique.
Lin
J M
JM
Department of Industrial Chemistry, Faculty of Engineering, Tokyo Metropolitan University, Japan.
Nakagama
T
T
Uchiyama
K
K
Hobo
T
T
D000596:Amino Acids / Q000737:chemistry / Q000302:isolation & purification*; D055598:Chemical Phenomena; D002627:Chemistry, Physical; D002845:Chromatography / Q000379:methods*; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D003432:Cross-Linking Reagents; D019075:Electrophoresis, Capillary; D008689:Methacrylates; D010649:Phenylalanine / Q000737:chemistry / Q000302:isolation & purification; D011108:Polymers / Q000737:chemistry*; D013237:Stereoisomerism; D013696:Temperature; D013816:Thermodynamics
D000596:Amino Acids; D003432:Cross-Linking Reagents; D008689:Methacrylates; D011108:Polymers; C008384:methacrylic acid; D010649:Phenylalanine; C004919:ethylene dimethacrylate