Long-term postnatal effect of prenatal irradiation on the astrocyte proliferative response to brain injury.
Pregnant Wistar rats were exposed to a single 1.0 Gy dose of gamma-irradiation on gestational day 13, 15, 17 or 19. Thirty-day-old male offspring received a mechanical lesion in the left cerebral hemisphere. One, 2 or 4 days after the injury the rats were injected with [3H]thymidine and sacrificed 4 h after the injection. Thereafter, brain sections were immunostained for GFAP or S100 beta protein, subjected to autoradiography and examined microscopically to record immunopositive astrocytes labelled with [3H]thymidine. Statistically significant elevation of the reactive astrocyte proliferation was revealed on the 2nd day following injury in brains irradiated on gestational day 15. The results represent the first in vivo evidence that a low-dose prenatal gamma-irradiation can induce a long-term increase in the ability of astroglia to proliferate in response to injury.
Janeczko
K
K
Department of Neuroanatomy, Jagiellonian University, Kraków, Poland. jane@zuk.iz.uj.edu.pl
Pawliński
R
R
Setkowicz
Z
Z
Ziaja
M
M
Sołtys
Z
Z
Ryszka
A
A
D000818:Animals; D001253:Astrocytes / Q000737:chemistry / Q000166:cytology* / Q000528:radiation effects*; D001345:Autoradiography; D001930:Brain Injuries / Q000503:physiopathology*; D002455:Cell Division / Q000528:radiation effects; D005260:Female; D005720:Gamma Rays; D005904:Glial Fibrillary Acidic Protein / Q000032:analysis; D007150:Immunohistochemistry; D008297:Male; D011247:Pregnancy; D011297:Prenatal Exposure Delayed Effects; D051381:Rats; D017208:Rats, Wistar; D009418:S100 Proteins / Q000032:analysis
D005904:Glial Fibrillary Acidic Protein; D009418:S100 Proteins