Influences of dietary methionine and cysteine on metabolic responses to immunological stress by Escherichia coli lipopolysaccharide injection, and mitogenic response in broiler chickens.
The present experiments were conducted to investigate influences of dietary methionine and cysteine on metabolic responses to immunological stress induced by Escherichia coli lipopolysaccharide (LPS) injection, and concanavalin A (Con A)-induced mononuclear cell (MNC) proliferation in male broiler chickens. In Expt 1, chicks (12 d of age) were fed on a S amino acid (SAA)-deficient diet (5.6 g SAA/kg diet) or on three kinds of SAA-sufficient diet (9.3 g SAA/kg diet; low-, medium- and high-cysteine diets) which contained 2.8, 4.65 and 6.5 g cysteine/kg diet, respectively. Plasma alpha-1 acid glycoprotein (AGP) concentration and interleukin (IL)-1-like activity in chicks fed on the SAA-deficient diet were lower following a single injection of LPS than those in chicks fed on the SAA-sufficient diets. At 16 h after LPS injection, plasma Fe and Zn concentrations and body weight were reduced, but AGP concentration and IL-1-like activity in plasma were significantly increased. These changes in body weight, plasma Zn and Fe concentrations following injection of LPS were not affected by dietary methionine: cysteine ratios. Plasma AGP concentration and IL-1-like activity in chicks fed on the high-cysteine diet were, however, greater than those in chicks fed on the other diets following a single injection of LPS. In Expt 2, chicks (7 d of age) were fed on the SAA-sufficient diets as in Expt 1 for 10 d. MNC proliferation in spleen induced by Con A in chicks fed on the high-cysteine diet was greater than that in chicks fed on the low- or medium-cysteine diet. The results suggest that dietary cysteine has an impact on the immune and inflammatory responses.
Takahashi
K
K
Department of Animal Science, Faculty of Agriculture, Tohoku University, Sendai-shi, Japan.
Ohta
N
N
Akiba
Y
Y
D000210:Acute-Phase Reaction; D000596:Amino Acids / Q000008:administration & dosage*; D000818:Animals; D001835:Body Weight; D002645:Chickens / Q000276:immunology* / Q000378:metabolism; D003208:Concanavalin A / Q000494:pharmacology; D003545:Cysteine / Q000008:administration & dosage; D004032:Diet; D004926:Escherichia coli; D007375:Interleukin-1 / Q000378:metabolism; D007501:Iron / Q000097:blood; D007963:Leukocytes, Mononuclear / Q000187:drug effects; D008070:Lipopolysaccharides / Q000008:administration & dosage*; D008297:Male; D008715:Methionine / Q000008:administration & dosage; D008934:Mitogens / Q000494:pharmacology; D011897:Random Allocation; D013154:Spleen / Q000276:immunology; D013950:Thymus Gland / Q000276:immunology; D015032:Zinc / Q000097:blood
D000596:Amino Acids; D007375:Interleukin-1; D008070:Lipopolysaccharides; D008934:Mitogens; D003208:Concanavalin A; D008715:Methionine; D007501:Iron; D015032:Zinc; D003545:Cysteine