title: Effects of natriuretic peptides and endothelins on the nerve-evoked release of adenine nucleotides and nucleosides in guinea-pig vas deferens.
abstract: Two members of the natriuretic peptide family (rANF8-33 and pBNP1-32) and two members of the endothelin family (ET-1 and ET-2) have been studied for their effects on the neurogenically induced overflow of adenosine 5'-triphosphate (ATP), adenosine 5'-diphosphate (ADP), adenosine 5'-monophosphate (AMP), and adenosine (ADO) from the isolated guinea-pig vas deferens. rANF, pBNP, ET-1, and ET-2 each at 10 nM produced a significant increase in the evoked overflow of ATP, by 52, 85, 130, and 115%, respectively. None of the peptides altered the overflow of ADO. ET-1 and ET-2 each caused an increase in the overflow of ADP and AMP by an amount similar to their effects on ATP overflow, so that the ratio ATP:ADP remained 1:1 throughout. Natriuretic peptides, however, affected the overflow of ADP and AMP to a lesser extent than ATP, resulting in an ATP:ADP ratio of 2:1 after rANF and of 1.5:1 after pBNP. In addition, rANF or pBNP, but not ET-1 or ET-2, inhibited ecto-ATPase activity, suggesting that this mechanism may contribute to the facilitatory effect of the natriuretic peptides on the nerve-evoked overflow of ATP in this tissue.
authors.affiliation: Department of Pharmacology, University of Nevada School of Medicine, Reno 89557, USA.
authors.forename: V N
authors.initials: VN
authors.lastname: Mutafova-Yambolieva
authors.forename: I A
authors.initials: IA
authors.lastname: Yamboliev
authors.forename: W T
authors.initials: WT
authors.lastname: Gerthoffer
authors.forename: D P
authors.initials: DP
authors.lastname: Westfall
mesh_terms: D000227:Adenine Nucleotides / Q000378:metabolism*; D000241:Adenosine / Q000378:metabolism*; D000251:Adenosine Triphosphatases / Q000378:metabolism; D000255:Adenosine Triphosphate / Q000378:metabolism*; D000818:Animals; D009320:Atrial Natriuretic Factor / Q000494:pharmacology*; D004558:Electric Stimulation; D019332:Endothelin-1 / Q000494:pharmacology; D019333:Endothelin-2 / Q000494:pharmacology; D016232:Endothelins / Q000494:pharmacology*; D006168:Guinea Pigs; D066298:In Vitro Techniques; D008297:Male; D009130:Muscle, Smooth / Q000201:enzymology / Q000294:innervation / Q000378:metabolism; D020097:Natriuretic Peptide, Brain; D009419:Nerve Tissue Proteins / Q000494:pharmacology*; D010446:Peptide Fragments / Q000494:pharmacology*; D014649:Vas Deferens / Q000201:enzymology / Q000294:innervation / Q000378:metabolism*
chemical_list: D000227:Adenine Nucleotides; D019332:Endothelin-1; D019333:Endothelin-2; D016232:Endothelins; D009419:Nerve Tissue Proteins; D010446:Peptide Fragments; D020097:Natriuretic Peptide, Brain; C076910:brain natriuretic peptide, porcine; D009320:Atrial Natriuretic Factor; D000255:Adenosine Triphosphate; C044120:atrial natriuretic factor prohormone (8-33); D000251:Adenosine Triphosphatases; C027201:ectoATPase; D000241:Adenosine