PGE2 inhibits glucose uptake in isolated villous epithelial cells of the ovine small intestine.
This study was designed to clarify whether PGE2 directly inhibits glucose absorption in villous cells. For this purpose, isolated villous epithelial cells of the ovine small intestine were used, and the uptake of 14C-labelled glucose in the absorptive epithelial cells was measured in the presence and absence of 0.2 mM phlorizin or 10 microM PGE2. In isolated villous epithelial cells of the small intestine in sheep, net glucose absorption was reduced by 68.2% in the presence of phlorizin. This result indicates that most of the glucose in ruminants is absorbed by the specific transporter SGLT1, which is sensitive to phlorizin. PGE2 decreased glucose absorption by 26.5% in isolated villous epithelial cells in sheep, although this glucose absorption did not change in the presence of phlorizin. This result clearly indicates the PGE2 inhibits the phlorizin-sensitive glucose absorptive process through direct action on the villous cells of ruminants.
Hyun
H S
HS
School of Veterinary Medicine, Chonnam National University, Kwang-Ju, Korea.
Arai
S
S
Onaga
T
T
Kato
S
S
D000818:Animals; D002478:Cells, Cultured; D015232:Dinoprostone / Q000494:pharmacology*; D004847:Epithelial Cells / Q000378:metabolism / Q000502:physiology / Q000648:ultrastructure; D005947:Glucose / Q000493:pharmacokinetics*; D006027:Glycosides / Q000494:pharmacology; D007408:Intestinal Absorption / Q000187:drug effects / Q000502:physiology; D007421:Intestine, Small / Q000166:cytology* / Q000378:metabolism* / Q000648:ultrastructure; D008297:Male; D008871:Microvilli / Q000502:physiology / Q000648:ultrastructure; D010695:Phlorhizin / Q000494:pharmacology; D012756:Sheep / Q000378:metabolism*
D006027:Glycosides; D010695:Phlorhizin; D005947:Glucose; D015232:Dinoprostone