Role of guanylyl cyclase receptors for CNP in salt secretion by shark rectal gland.
The role of C-type natriuretic peptide (CNP) and its guanylyl cyclase-linked receptors in mediating salt secretion by the rectal gland of the spiny dogfish shark (Squalus acanthias) was investigated using HS-142-1, a competitive inhibitor of the binding of natriuretic peptides to their guanylyl cyclase receptors. CNP binds to receptors and activates guanylyl cyclase in rectal gland membranes in a way that is inhibited by HS-142-1. Guanylyl cyclase activation in rectal gland membranes is far more sensitive to CNP than to atrial natriuretic peptide, whereas the reverse is true for membranes derived from mammalian (rabbit) renal collecting duct cells. HS-142-1 inhibited the stimulatory effect of CNP on ouabain-inhibitable oxygen consumption by rectal gland tubules. In explanted rectal glands continuously perfused with blood from intact donor sharks, HS-142-1 inhibited the increase in salt secretion normally provoked by infusing isotonic saline solutions into the donor animal. These results strongly support the view that CNP released into the systemic circulation in response to volume expansion mediates the secretion of chloride by the rectal gland via receptors linked to guanylyl cyclase.
Gunning
M
M
Department of Medicine, Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, Massachusetts 02215, USA.
Solomon
R J
RJ
Epstein
F H
FH
Silva
P
P
D000262:Adenylyl Cyclases / Q000378:metabolism; D000818:Animals; D002462:Cell Membrane / Q000378:metabolism; D002712:Chlorides / Q000378:metabolism; D005260:Female; D006162:Guanylate Cyclase / Q000378:metabolism / Q000502:physiology*; D006801:Humans; D007552:Isotonic Solutions / Q000494:pharmacology; D008297:Male; D045283:Natriuretic Agents / Q000494:pharmacology; D020098:Natriuretic Peptide, C-Type; D010042:Ouabain / Q000494:pharmacology; D010101:Oxygen Consumption / Q000187:drug effects; D011134:Polysaccharides / Q000494:pharmacology; D011506:Proteins / Q000378:metabolism; D017461:Receptors, Atrial Natriuretic Factor / Q000502:physiology*; D012491:Salt Gland / Q000378:metabolism*; D012754:Sharks / Q000378:metabolism*; D012965:Sodium Chloride / Q000378:metabolism*
D002712:Chlorides; C072551:HS 142-1; D007552:Isotonic Solutions; D045283:Natriuretic Agents; D011134:Polysaccharides; D011506:Proteins; D020098:Natriuretic Peptide, C-Type; D012965:Sodium Chloride; D010042:Ouabain; D000262:Adenylyl Cyclases; D006162:Guanylate Cyclase; D017461:Receptors, Atrial Natriuretic Factor; C073616:atrial natriuretic factor receptor C