The effect of smoking on serum IgG2 reactive with Actinobacillus actinomycetemcomitans in early-onset periodontitis patients.
High titers of serum IgG2 reactive with Actinobacillus actinomycetemcomitans are present in early-onset periodontitis (EOP) patients and it appears that anti-A. actinomycetemcomitans may be protective. Smoking is associated with increased periodontal disease severity in generalized early-onset periodontitis (G-EOP) patients, but is not associated with periodontal disease severity in patients with localized juvenile periodontitis (LJP). Furthermore, smoking is associated with reduced serum IgG2 levels in black patients with G-EOP but not in those with LJP. Based on this selective effect of smoking, we hypothesized that smoking would be associated with a reduction of specific IgG2 reactive with A. actinomycetemcomitans in black G-EOP patients but not black LJP patients. In addition, we examined IgG2 responses to carbohydrate antigens from non-periodontal pathogens including Haemophilus influenzae b oligosaccharide antigen (Hib) and the Streptococcus pneumoniae antigen phosphocholine (PC). Smoking status was assessed from serum cotinine levels, and IgG2 specific for A. actinomycetemcomitans, Hib, and PC was assessed by ELISA. Our study revealed that smoking was correlated with a dramatic reduction in serum IgG2 anti-A. actinomycetemcomitans in G-EOP smokers but not in LJP smokers. In contrast, anti-Hib IgG2 and anti-PC IgG2 were not affected in either G-EOP or LJP patients. In short, these results indicate that smoking is associated with a reduction in serum IgG2 anti-A. actinomycetemcomitans in black G-EOP subjects, but IgG2 reactive with other antigens may not be reduced in G-EOP smokers.
Tangada
S D
SD
Clinical Research Center for Periodontal Diseases, School of Dentistry, Medical College of Virginia/Virginia Commonwealth University, Richmond, USA.
Califano
J V
JV
Nakashima
K
K
Quinn
S M
SM
Zhang
J B
JB
Gunsolley
J C
JC
Schenkein
H A
HA
Tew
J G
JG
D000293:Adolescent; D000328:Adult; D016976:Aggregatibacter actinomycetemcomitans / Q000276:immunology*; D010520:Aggressive Periodontitis / Q000276:immunology* / Q000382:microbiology / Q000473:pathology; D000907:Antibodies, Bacterial / Q000097:blood*; D000942:Antigens, Bacterial / Q000276:immunology; D003367:Cotinine / Q000097:blood; D003429:Cross Reactions; D003774:Dental Plaque Index; D004797:Enzyme-Linked Immunosorbent Assay; D006193:Haemophilus influenzae / Q000276:immunology; D006801:Humans; D007074:Immunoglobulin G / Q000097:blood*; D009538:Nicotine / Q000378:metabolism; D009844:Oligosaccharides / Q000276:immunology; D017622:Periodontal Attachment Loss / Q000473:pathology; D010512:Periodontal Index; D010518:Periodontitis / Q000276:immunology* / Q000382:microbiology / Q000473:pathology; D010767:Phosphorylcholine / Q000276:immunology; D012907:Smoking / Q000097:blood / Q000276:immunology*; D013296:Streptococcus pneumoniae / Q000276:immunology
D000907:Antibodies, Bacterial; D000942:Antigens, Bacterial; D007074:Immunoglobulin G; D009844:Oligosaccharides; D010767:Phosphorylcholine; D009538:Nicotine; D003367:Cotinine