title: Sex and depot-specific stimulation of creatine kinase B in rat adipose tissues by gonadal steroids.
abstract: We report a sex- and depot-specific response of rat adipose tissues to gonadal steroids. The epididymal fat pad in male rats responded to androgens (testosterone and dihydrotestosterone; DHT), but not to 17beta-estradiol (E2), by increased specific activity of the brain type isozyme of creatine kinase (CK). In female rats, the parametrial fat as well as the fat surrounding the spleen responded to E2 but not to dihydrotestosterone. In both sexes, subcutaneous fat from the inguinal, abdominal or thigh region did not respond to any sex steroid. The parametrial fat showed increasing responsiveness to E2 during postnatal development, in parallel to the response of the uterus. In cycling female rats, parametrial fat showed the highest basal activity of CK at estrus; stimulation by E2 was achieved on all the other days of the cycle. Both phytoestrogens and diethylstilbestrol stimulated CK activity in both parametrial and spleen fat, in parallel to their estrogenic potencies; parametrial fat also responded to progesterone. The stimulation of CK activity in parametrial fat by E2 was completely blocked by actinomycin D or cycloheximide. Treatment with the antiestrogen, tamoxifen, caused moderate stimulation of CK activity in parametrial fat as well as partial inhibition of E2 stimulation of CK activity; the "pure" antiestrogen ICI 164,384 had no agonistic effect and completely blocked the E2 effect. Ovariectomy caused an increased response to E2 without changes in the basal CK activity, but did not lead to any response to DHT. As well as being a reliable response marker, the differential modulation of CK activity can thus serve to distinguish adipose cells from different sources.
authors.affiliation: Tel-Aviv Sourasky Medical Center and the Sackler Faculty of Medicine, Tel Aviv University, Israel.
authors.forename: D
authors.initials: D
authors.lastname: Sömjen
authors.forename: S
authors.initials: S
authors.lastname: Lundgren
authors.forename: A M
authors.initials: AM
authors.lastname: Kaye
mesh_terms: D000273:Adipose Tissue / Q000033:anatomy & histology* / Q000201:enzymology* / Q000254:growth & development; D000375:Aging / Q000378:metabolism*; D000818:Animals; D001921:Brain / Q000201:enzymology; D003402:Creatine Kinase / Q000096:biosynthesis / Q000378:metabolism*; D004054:Diethylstilbestrol / Q000494:pharmacology; D013196:Dihydrotestosterone / Q000494:pharmacology*; D004790:Enzyme Induction; D004822:Epididymis; D004958:Estradiol / Q000494:pharmacology*; D004968:Estrogens, Non-Steroidal / Q000494:pharmacology; D004971:Estrus; D005260:Female; D007527:Isoenzymes; D007529:Isoflavones; D008297:Male; D010052:Ovariectomy; D048789:Phytoestrogens; D028321:Plant Preparations; D051381:Rats; D017208:Rats, Wistar; D012727:Sex Characteristics; D013154:Spleen; D013739:Testosterone / Q000494:pharmacology*; D014599:Uterus / Q000201:enzymology / Q000254:growth & development
chemical_list: D004968:Estrogens, Non-Steroidal; D007527:Isoenzymes; D007529:Isoflavones; D048789:Phytoestrogens; D028321:Plant Preparations; D013196:Dihydrotestosterone; D013739:Testosterone; D004958:Estradiol; D004054:Diethylstilbestrol; D003402:Creatine Kinase