title: High-performance liquid chromatographic assay of arachidonic acid metabolites from cultured rat glial cells.
abstract: Although various methods can be used for detection of arachidonic acid (AA) metabolites, only few of them allow rapid and precise analysis of both cyclooxygenase and lipoxygenase metabolites in a single assay. We have developed a simple and rapid method to evaluate overall profiles of AA metabolites produced by primary cultured glial cells from newborn rats by using reversed-phase, high-performance liquid chromatography (RP-HPLC) and on-line radioisotope detection.
authors.affiliation: Department of Pediatrics, Saga Medical School, Japan.
authors.forename: M
authors.initials: M
authors.lastname: Matsuo
authors.forename: Y
authors.initials: Y
authors.lastname: Hamasaki
authors.forename: S
authors.initials: S
authors.lastname: Miyazaki
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D016718:Arachidonic Acid / Q000378:metabolism*; D002478:Cells, Cultured; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D009457:Neuroglia / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar
chemical_list: D016718:Arachidonic Acid