title: Stage-specific serine and metallo-proteinase release by adult and larval Trichostrongylus vitrinus.
abstract: Proteinases were released in a stage-specific manner during in vitro culture by 4th larval stage and adult Trichostrongylus vitrinus. Substrate gel analyses and inhibitor studies revealed the presence of serine and metallo-proteinases, active over a broad pH range, which degraded proteins such as fibrinogen, plasminogen and fibronectin but not immunoglobulin. The adult proteinases were partially inhibited (43%) by immunoglobulin from immune lamb lymph compared to controls, indicating their relevance to parasite immunobiology in vivo.
authors.affiliation: Moredun Research Institute, Edinburgh, U.K.
authors.forename: K
authors.initials: K
authors.lastname: MacLennan
authors.forename: M P
authors.initials: MP
authors.lastname: Gallagher
authors.forename: D P
authors.initials: DP
authors.lastname: Knox
mesh_terms: D000818:Animals; D005353:Fibronectins / Q000378:metabolism; D007814:Larva / Q000201:enzymology; D008666:Metalloendopeptidases / Q000378:metabolism*; D010958:Plasminogen / Q000378:metabolism; D011480:Protease Inhibitors / Q000494:pharmacology; D012697:Serine Endopeptidases / Q000378:metabolism*; D013379:Substrate Specificity; D014254:Trichostrongylus / Q000201:enzymology* / Q000254:growth & development
chemical_list: D005353:Fibronectins; D011480:Protease Inhibitors; D010958:Plasminogen; D012697:Serine Endopeptidases; D008666:Metalloendopeptidases