title: Chronic diazepam exposure decreases transcription of the rat GABA(A) receptor gamma2-subunit gene.
abstract: The rate of transcription of the GABA(A) receptor gamma2-subunit gene in rat cortex has been measured using the nuclear run-off transcriptional assay. Exposure of rats to diazepam (15 mg/kg/day for 14 days) caused a significant reduction in the level of nascent GABA(A) receptor gamma2-subunit transcripts. Therefore, a component of the cellular response to chronic benzodiazepine exposure includes events which take place at the level of transcription of a GABA(A) receptor gene.
authors.affiliation: Department of Pharmacology, University of Alberta, Edmonton, Canada. rholt@gpu.srv.ualberta.ca
authors.forename: R A
authors.initials: RA
authors.lastname: Holt
authors.forename: I L
authors.initials: IL
authors.lastname: Martin
authors.forename: A N
authors.initials: AN
authors.lastname: Bateson
mesh_terms: D000818:Animals; D014151:Anti-Anxiety Agents / Q000494:pharmacology*; D002467:Cell Nucleus / Q000187:drug effects / Q000378:metabolism; D003975:Diazepam / Q000494:pharmacology*; D015536:Down-Regulation / Q000187:drug effects / Q000502:physiology*; D004334:Drug Administration Schedule; D046911:Macromolecular Substances; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley; D011963:Receptors, GABA-A / Q000096:biosynthesis* / Q000737:chemistry; D013997:Time Factors; D014158:Transcription, Genetic / Q000187:drug effects*
chemical_list: D014151:Anti-Anxiety Agents; D046911:Macromolecular Substances; D011963:Receptors, GABA-A; D003975:Diazepam