title: Blue and yellow laccases of ligninolytic fungi.
abstract: Extracellular laccases from submerged cultures of Coriolus versicolor BKM F-116, Panus tigrinus 8/18, Phlebia radiata 79 (ATCC 64658), Phlebia tremellosa 77-51 and from cultures of Pa. tigrinus 8/18, Ph. radiata 79 and Agaricus bisporus D-649 grown on wheat straw (solid-state fermentation) were purified. All enzymes from submerged cultures had a blue colour and characteristic absorption and EPR spectra. Laccases from the solid-state cultures were yellow-brown and had no typical blue oxidase spectra and also showed atypical EPR spectra. Comparison of N-terminal amino acid sequences of purified laccases showed high homology between blue and yellow-brown laccase forms. Formation of yellow laccases as a result of binding of lignin-derived molecules by enzyme protein is proposed.
authors.affiliation: Institute of Biochemistry and Physiology of Microorganisms, Russian Academy of Sciences, Pushchino, Moscow Region, Russia.
authors.forename: A A
authors.initials: AA
authors.lastname: Leontievsky
authors.forename: T
authors.initials: T
authors.lastname: Vares
authors.forename: P
authors.initials: P
authors.lastname: Lankinen
authors.forename: J K
authors.initials: JK
authors.lastname: Shergill
authors.forename: N N
authors.initials: NN
authors.lastname: Pozdnyakova
authors.forename: N M
authors.initials: NM
authors.lastname: Myasoedova
authors.forename: N
authors.initials: N
authors.lastname: Kalkkinen
authors.forename: L A
authors.initials: LA
authors.lastname: Golovleva
authors.forename: R
authors.initials: R
authors.lastname: Cammack
authors.forename: C F
authors.initials: CF
authors.lastname: Thurston
authors.forename: A
authors.initials: A
authors.lastname: Hatakka
mesh_terms: D000364:Agaricus / Q000201:enzymology / Q000235:genetics; D000595:Amino Acid Sequence; D003116:Color; D004578:Electron Spin Resonance Spectroscopy; D005658:Fungi / Q000201:enzymology* / Q000235:genetics; D042845:Laccase; D008031:Lignin / Q000378:metabolism*; D008969:Molecular Sequence Data; D010088:Oxidoreductases / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification*; D011124:Polyporaceae / Q000201:enzymology / Q000235:genetics; D017386:Sequence Homology, Amino Acid; D013053:Spectrophotometry; D013056:Spectrophotometry, Ultraviolet
chemical_list: D008031:Lignin; D010088:Oxidoreductases; D042845:Laccase