Expression of telomerase RNA, telomerase activity, and telomere length in human gliomas.
To understand the mechanisms of telomere maintenance in human gliomas, telomerase activity, telomerase RNA expression and telomere length of surgically excised glioma samples were analyzed. Sixty-five percent of gliomas exhibited telomerase activity, the occurrence of which was not related to their histological malignancy scale. Not only the telomerase-positive gliomas, but also the telomerase-negative gliomas and normal brain expressed telomerase RNA, suggesting that the presence of telomerase RNA component does not indicate the presence of telomerase activity. Compared with telomerase-positive gliomas, telomerase-negative gliomas had long heterogeneous telomeric terminal restriction fragments. These data suggest that in addition to the telomerase-dependent mechanism, a telomerase-independent mechanism for telomere maintenance may be present in human gliomas.
Morii
K
K
Department of Neurosurgery, Niigata University, Japan.
Tanaka
R
R
Onda
K
K
Tsumanuma
I
I
Yoshimura
J
J
D001254:Astrocytoma / Q000201:enzymology* / Q000235:genetics; D015139:Blotting, Southern; D001932:Brain Neoplasms / Q000201:enzymology* / Q000235:genetics; D004789:Enzyme Activation / Q000235:genetics; D005909:Glioblastoma / Q000201:enzymology* / Q000235:genetics; D006801:Humans; D008297:Male; D008875:Middle Aged; D016133:Polymerase Chain Reaction; D012334:RNA, Neoplasm / Q000096:biosynthesis*; D019098:Telomerase / Q000096:biosynthesis / Q000235:genetics* / Q000378:metabolism; D016615:Telomere / Q000737:chemistry* / Q000201:enzymology / Q000235:genetics
D012334:RNA, Neoplasm; D019098:Telomerase