Interactive suppression of aberrant crypt foci induced by azoxymethane in rat colon by phytic acid and green tea.
Several epidemiological studies point to a strong correlation between nutrient composition of the diet and cancer of the colon. Phytic acid, present in grains, has been credited with reducing the risk of cancer of the colon. A number of reports are available indicating the benefits of green tea consumption in reducing the risk of stomach, lung and skin cancer, but little data are available on the effect of green tea in reducing the risk of colon cancer. Also, there are no studies on the combined effect of these compounds on colon tumorigenesis. Thus the primary objective of this investigation was to elucidate the combined effects of green tea and phytic acid on colonic preneoplastic lesions and the Phase II enzyme glutathione S-transferase. Fisher 344 male weanling rats were divided into nine groups of 15 rats each and fed the experimental diet for 13 weeks. Rats received two s.c. injections of azoxymethane in saline at 16 mg/kg body wt at 7 and 8 weeks of age. Rats received three levels (0, 1 and 2%) of phytic acid with three levels (0, 1 and 2%) of green tea within each phytic acid level in a 3 x 3 factorial experiment. Results indicate that while green tea had a marginal effect (P < 0.14), phytic acid significantly reduced the incidence of aberrant crypt foci (P < 0.008). The interaction between green tea and phytic acid was significant (P < 0.029 for distal and < 0.0168 for entire colon) and positive, pointing to a synergistic effect of green tea and phytic acid.
Challa
A
A
Department of Food Science and Animal Industries, Alabama A&M University, Normal 35762, USA.
Rao
D R
DR
Reddy
B S
BS
D000704:Analysis of Variance; D000818:Animals; D016588:Anticarcinogenic Agents / Q000627:therapeutic use*; D001397:Azoxymethane / Q000633:toxicity; D002273:Carcinogens / Q000633:toxicity; D003106:Colon / Q000187:drug effects* / Q000201:enzymology / Q000473:pathology; D004354:Drug Screening Assays, Antitumor; D005982:Glutathione Transferase / Q000378:metabolism; D008297:Male; D010833:Phytic Acid / Q000627:therapeutic use*; D008517:Phytotherapy; D051381:Rats; D011916:Rats, Inbred F344; D013662:Tea / Q000627:therapeutic use*
D016588:Anticarcinogenic Agents; D002273:Carcinogens; D013662:Tea; D010833:Phytic Acid; D005982:Glutathione Transferase; D001397:Azoxymethane