Detection of varicella zoster virus DNA in single trigeminal ganglia by polymerase chain reaction.
Single human trigeminal ganglia were studied for DNA sequences of varicella zoster virus immediate early gene 63 by PCR. Out of 24 trigeminal ganglia five were tested positive for VZV DNA by standard PCR (21%), in six more VZV DNA was detectable using nested PCR (46%).
Mainka
C
C
Institute of Microbiology and Virology, University Witten/Herdecke, Germany.
Wolff
M H
MH
D000368:Aged; D000369:Aged, 80 and over; D015139:Blotting, Southern; D004279:DNA, Viral / Q000032:analysis*; D006562:Herpes Zoster / Q000821:virology; D014645:Herpesvirus 3, Human / Q000235:genetics* / Q000302:isolation & purification*; D006801:Humans; D008875:Middle Aged; D016133:Polymerase Chain Reaction / Q000379:methods; D012668:Trigeminal Ganglion / Q000737:chemistry / Q000821:virology*
D004279:DNA, Viral