Expression of a novel splice variant of human mGluR1 in the cerebellum.
We have isolated clones of a novel splice variant of metabotropic glutamate receptor type 1 (mGluR1) from a human cerebellum cDNA library. Translation of this variant, mGluR1g would result in the addition of just one amino acid after the exon/intron boundary where the other splice variants diverge. RNA dot blot analysis using an mGluR1g-specific probe demonstrated expression in the cerebellum and also high levels in the kidney. Northern blotting using the same probe showed expression of a 4 kb transcript in the cerebellum. In situ hybridization studies in the cerebellum showed that mGluR1g mRNA is only expressed in granule cells, compared with mGluR1a/b mRNA which is also found in Purkinje cells and basket cells. Transcripts of the analogous splice variant are also present in rat brain mRNA.
Makoff
A J
AJ
Department of Psychological Medicine, Institute of Psychiatry, Denmark Hill, London, UK.
Phillips
T
T
Pilling
C
C
Emson
P
P
D017398:Alternative Splicing; D000818:Animals; D001483:Base Sequence; D002531:Cerebellum / Q000737:chemistry*; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000302:isolation & purification*; D005091:Exons; D006801:Humans; D007438:Introns; D008969:Molecular Sequence Data; D051381:Rats; D018094:Receptors, Metabotropic Glutamate / Q000235:genetics*; D012689:Sequence Homology, Nucleic Acid
D018076:DNA, Complementary; D018094:Receptors, Metabotropic Glutamate