Hypertonicity enhances expression of functional Na+/K+/2Cl- cotransporters in Ehrlich ascites tumour cells.
Ehrlich cells exposed to a hypertonic medium for five hours respond by an increased expression of Na+/K+/2Cl- cotransport proteins as estimated from immunoprecipitations using polyclonal anti-cotransporter antibodies. The 3.4-fold increase in cotransport expression is followed by a concomitant 2.6-fold increase in the maximal bumetanide-sensitive K+ influx during regulatory volume increase, indicating a 2.6-fold increase in the number of functional cotransporters in the plasma membrane.
Jensen
B S
BS
NeuroSearch, Glostrup, Denmark.
Hoffmann
E K
EK
D000818:Animals; D000906:Antibodies / Q000276:immunology / Q000378:metabolism / Q000494:pharmacology; D002034:Bumetanide / Q000494:pharmacology; D002352:Carrier Proteins / Q000276:immunology / Q000378:metabolism*; D002712:Chlorides / Q000378:metabolism*; D004591:Electrophoresis, Polyacrylamide Gel; D005786:Gene Expression Regulation / Q000235:genetics; D006982:Hypertonic Solutions / Q000494:pharmacology*; D017136:Ion Transport; D009994:Osmolar Concentration; D009997:Osmotic Pressure; D011188:Potassium / Q000378:metabolism*; D011233:Precipitin Tests; D012414:Rubidium Radioisotopes / Q000378:metabolism; D012964:Sodium / Q000378:metabolism*; D028021:Sodium-Potassium-Chloride Symporters; D014407:Tumor Cells, Cultured
D000906:Antibodies; D002352:Carrier Proteins; D002712:Chlorides; D006982:Hypertonic Solutions; D012414:Rubidium Radioisotopes; D028021:Sodium-Potassium-Chloride Symporters; D002034:Bumetanide; D012964:Sodium; D011188:Potassium