title: Concurrent fluorescence in situ hybridization and immunocytochemistry for the detection of chromosome aberrations in exfoliated bronchial epithelial cells.
abstract: OBJECTIVE
  A procedure was developed to allow concurrent detection of chromosome aberrations and identification of bronchial epithelial cells.
  
  
  METHODS
  Fluorescence in situ hybridization for chromosome 7 and immunocytochemistry for cytokeratin were performed on exfoliated bronchial epithelial cells in a sputum sample from a cancer patient.
  
  
  RESULTS
  The Spectrum Orange-labeled alpha satellite probe for chromosome 7 produced red fluorescence, nuclei were counterstained with 4,6-diamidino-2-phenylindole (blue), and cytokeratin was visualized using a fluorescein isothiocyanate (FITC)-conjugated secondary antibody (green).
  
  
  CONCLUSIONS
  This procedure allowed the rapid identification of airway epithelial cells with numerical chromosome aberrations in this sample. Ultimately, this procedure could increase the sensitivity and specificity of sputum cytology as a laboratory diagnostic tool for the early detection of lung cancer.
authors.affiliation: Lovelace Respiratory Research Institute, Albuquerque, New Mexico 87185, USA.
authors.forename: R E
authors.initials: RE
authors.lastname: Neft
authors.forename: M M
authors.initials: MM
authors.lastname: Murphy
authors.forename: L A
authors.initials: LA
authors.lastname: Tierney
authors.forename: S A
authors.initials: SA
authors.lastname: Belinsky
authors.forename: M
authors.initials: M
authors.lastname: Anderson
authors.forename: G
authors.initials: G
authors.lastname: Saccomanno
authors.forename: R
authors.initials: R
authors.lastname: Michels
authors.forename: S
authors.initials: S
authors.lastname: Timm
authors.forename: F D
authors.initials: FD
authors.lastname: Gilliland
authors.forename: R E
authors.initials: RE
authors.lastname: Crowell
authors.forename: J F
authors.initials: JF
authors.lastname: Lechner
mesh_terms: D000911:Antibodies, Monoclonal; D001980:Bronchi / Q000473:pathology*; D002869:Chromosome Aberrations; D002897:Chromosomes, Human, Pair 7; D004276:DNA, Satellite; D004847:Epithelial Cells / Q000473:pathology; D006801:Humans; D007150:Immunohistochemistry / Q000379:methods; D017404:In Situ Hybridization, Fluorescence / Q000379:methods; D008175:Lung Neoplasms / Q000235:genetics* / Q000473:pathology*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013183:Sputum / Q000166:cytology*
chemical_list: D000911:Antibodies, Monoclonal; D004276:DNA, Satellite