Characterization of the long-lasting activator protein-1 complex induced by kainic acid treatment.
Kainic acid is known to induce seizures, neuronal damage and cell loss in the rat hippocampus. Our laboratory has shown that a single kainic acid injection elicits acute increases of activator protein-1 DNA-binding activity and this activity stays at an elevated level for 2 weeks after kainic acid injection. However, some pathological changes such as mossy fiber sprouting do not occur until 2-3 weeks after the kainic acid injection and the specific transcription factors regulating the long-term events after kainic acid treatment are not clear. To determine the involvement of activator protein-1 transcription factors in the long-term events after kainic acid treatment, gel mobility-shift and Western blot analyses were used. The results showed that two activator protein-1 complexes with different mobilities occur during the acute stage. However, only the faster-migrating complex as well as the 35-37-kDa fos-related antigen and Jun-D proteins were seen during the late stage. These results suggest that different activator protein-1 complexes exist at different stages after convulsions and that they regulate ensembles of different genes.
Feng
Z
Z
Neuropharmacology Section, National Institute of Environmental Health Sciences, National Institutes of Health, Research Triangle Park, NC 27709, USA. feng@niehs.nih.gov
Zhang
W
W
Hudson
P
P
Bing
G
G
Feng
W
W
Hong
J S
JS
D000818:Animals; D000918:Antibody Specificity; D015153:Blotting, Western; D018690:Excitatory Amino Acid Agonists; D006624:Hippocampus / Q000737:chemistry / Q000378:metabolism; D007608:Kainic Acid; D008297:Male; D010446:Peptide Fragments / Q000032:analysis / Q000276:immunology / Q000378:metabolism; D016760:Proto-Oncogene Proteins c-fos / Q000032:analysis / Q000378:metabolism; D016755:Proto-Oncogene Proteins c-jun / Q000032:analysis / Q000276:immunology / Q000378:metabolism; D051381:Rats; D011916:Rats, Inbred F344; D012640:Seizures / Q000139:chemically induced / Q000378:metabolism*; D013997:Time Factors; D018808:Transcription Factor AP-1 / Q000032:analysis / Q000737:chemistry / Q000378:metabolism*
D018690:Excitatory Amino Acid Agonists; D010446:Peptide Fragments; D016760:Proto-Oncogene Proteins c-fos; D016755:Proto-Oncogene Proteins c-jun; D018808:Transcription Factor AP-1; D007608:Kainic Acid