Decreased IgG-Fc gamma RII dissociation kinetics in the presence of a protein antigen.
Total internal reflection fluorescence microscopy has been used to examine the interaction of a mouse monoclonal IgG2b, in the absence and presence of its protein antigen, with mouse Fc gamma RII in substrate-supported planar membranes. Equilibrium association and kinetic dissociation constants were measured for the antibody S6-34.11, which is specific for bovine prothrombin fragment 1 (BF1). These measurements showed that BF1 induces a statistically significant decrease (30-40%) in the IgG-Fc gamma RII dissociation kinetics. A corresponding increase in the equilibrium association constant was not observed, perhaps because the statistical accuracy of the equilibrium measurements is lower than that for the kinetic measurements. The consequences of these results for understanding the mechanism by which macrophages recognize and ingest opsonized targets are discussed.
Sheets
E D
ED
Department of Chemistry, University of North Carolina, Chapel Hill 27599-3290, USA.
Chen
L
L
Thompson
N L
NL
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology / Q000378:metabolism*; D000937:Antigen-Antibody Reactions; D001665:Binding Sites; D002417:Cattle; D002460:Cell Line; D002462:Cell Membrane / Q000378:metabolism; D006825:Hybridomas / Q000276:immunology; D007074:Immunoglobulin G / Q000276:immunology / Q000378:metabolism*; D007700:Kinetics; D051379:Mice; D008856:Microscopy, Fluorescence; D008970:Molecular Weight; D010446:Peptide Fragments / Q000276:immunology*; D011498:Protein Precursors / Q000276:immunology*; D011516:Prothrombin / Q000276:immunology*; D051381:Rats; D017452:Receptors, IgG / Q000378:metabolism*; D013499:Surface Properties
D000911:Antibodies, Monoclonal; D007074:Immunoglobulin G; D010446:Peptide Fragments; D011498:Protein Precursors; D017452:Receptors, IgG; C029733:prothrombin fragment 1; D011516:Prothrombin