Application of micropreparative capillary electrophoresis and polymer gel desalting techniques to biological samples illustrated using human synovial fluid.
A component of human synovial fluid (SF) has been separated by micropreparative capillary electrophoresis. The problems associated with application of this technique to a raw body fluid are discussed. Desalting of SF by passage through a capillary formed from polyacrylamide gel is examined and shown to cause loss of hyaluronan polymer as well as low-molecular-mass components of the fluid.
Grimshaw
J
J
School of Chemistry, Queen's University of Belfast, Northern Ireland.
Rice
A
A
Trocha-Grimshaw
J
J
D000180:Acrylic Resins; D001172:Arthritis, Rheumatoid / Q000378:metabolism*; D001826:Body Fluids / Q000737:chemistry; D019075:Electrophoresis, Capillary / Q000295:instrumentation / Q000379:methods; D005782:Gels; D006801:Humans; D008832:Microchemistry / Q000379:methods; D008970:Molecular Weight; D012492:Salts; D013396:Suction; D013582:Synovial Fluid / Q000737:chemistry*
D000180:Acrylic Resins; D005782:Gels; D012492:Salts; C016680:polyacrylamide gels