CD1d-restricted and TCR-mediated activation of valpha14 NKT cells by glycosylceramides.
Natural killer T (NKT) lymphocytes express an invariant T cell antigen receptor (TCR) encoded by the Valpha14 and Jalpha281 gene segments. A glycosylceramide-containing alpha-anomeric sugar with a longer fatty acyl chain (C26) and sphingosine base (C18) was identified as a ligand for this TCR. Glycosylceramide-mediated proliferative responses of Valpha14 NKT cells were abrogated by treatment with chloroquine-concanamycin A or by monoclonal antibodies against CD1d/Vbeta8, CD40/CD40L, or B7/CTLA-4/CD28, but not by interference with the function of a transporter-associated protein. Thus, this lymphocyte shares distinct recognition systems with either T or NK cells.
Kawano
T
T
CREST (Core Research for Evolutional Science and Technology) Project, Japan Science and Technology Corporation (JST), 1-8-1 Inohana, Chuo, Chiba 260, Japan.
Cui
J
J
Koezuka
Y
Y
Toura
I
I
Kaneko
Y
Y
Motoki
K
K
Ueno
H
H
Nakagawa
R
R
Sato
H
H
Kondo
E
E
Koseki
H
H
Taniguchi
M
M
D000818:Animals; D018949:Antigens, CD1 / Q000276:immunology*; D002236:Carbohydrate Conformation; D002478:Cells, Cultured; D002518:Ceramides / Q000737:chemistry / Q000378:metabolism / Q000494:pharmacology*; D002554:Cerebrosides / Q000737:chemistry / Q000378:metabolism / Q000494:pharmacology*; D018920:Coculture Techniques; D005699:Galactosylceramides / Q000737:chemistry / Q000378:metabolism / Q000494:pharmacology; D005963:Glucosylceramides / Q000737:chemistry / Q000378:metabolism / Q000494:pharmacology; D007694:Killer Cells, Natural / Q000276:immunology*; D008024:Ligands; D008213:Lymphocyte Activation; D051379:Mice; D008810:Mice, Inbred C57BL; D008822:Mice, Transgenic; D016693:Receptors, Antigen, T-Cell, alpha-beta / Q000276:immunology*; D013329:Structure-Activity Relationship; D016176:T-Lymphocyte Subsets / Q000276:immunology*
D018949:Antigens, CD1; D002518:Ceramides; D002554:Cerebrosides; D005699:Galactosylceramides; D005963:Glucosylceramides; D008024:Ligands; D016693:Receptors, Antigen, T-Cell, alpha-beta