Immunochemical characterization of lipopolysaccharide from glucose-nonfermenting gram-negative clinical bacterial isolate.
A glucose-nonfermenting Gram-negative bacterial strain isolated from bronchofiberoscope used for examination of the patients suffering from pulmonary diseases was subjected to phenol-water extraction. Lipopolysaccharides (LPS) isolated from the water and the phenol phase differed in fatty acid composition. Both contained xylose, glucose, glucosamine and components typical for LPS, namely heptose, 3-deoxyoctulosonic acid (Kdo) and 3-hydroxymyristic acid. The presence of sphingosine in all LPS preparations classifies the strain to the genus Sphingomonas.
Mieszała
M
M
Institute of Immunology and Experimental Therapy, Polish Academy of Sciences, Wrocław, Poland.
Kübler
J
J
Gamian
A
A
D000818:Animals; D018893:Bronchoalveolar Lavage; D055598:Chemical Phenomena; D002627:Chemistry, Physical; D004591:Electrophoresis, Polyacrylamide Gel; D005285:Fermentation; D005417:Flavobacterium / Q000737:chemistry / Q000145:classification / Q000378:metabolism; D005947:Glucose / Q000378:metabolism*; D006088:Gram-Negative Aerobic Bacteria / Q000737:chemistry* / Q000145:classification* / Q000378:metabolism; D006801:Humans; D007150:Immunohistochemistry; D008070:Lipopolysaccharides / Q000032:analysis* / Q000302:isolation & purification; D019800:Phenol / Q000737:chemistry; D011817:Rabbits; D014867:Water / Q000737:chemistry
D008070:Lipopolysaccharides; D014867:Water; D019800:Phenol; D005947:Glucose