The HMC-1 human mast cell line expresses the hepatocyte growth factor receptor c-met.
Hepatocyte growth factor (HGF) was originally characterized as a strong inducer of liver regeneration. However, it is now clear that HGF and its receptor, the proto-oncogene c-met, can be expressed in many other tissues, and that HGF can mediate diverse biological activities. We investigated the expression and function of c-met in a human mast cell line (HMC-1). We found that HMC-1 cells express c-met and that c-met expression can be upregulated by treatment of the cells with phorbol 12-myristate 13-acetate (PMA). Although HGF did not detectably influence the proliferation or morphology of HMC-1 cells, HGF inhibited the cells' ability to release tumor necrosis factor-alpha (TNF-alpha) in response to stimulation with PMA and the calcium ionophore, A23187. These results add the inhibition of TNF-alpha production to the other recognized effects of HGF/c-met on cellular function.
Yano
K
K
First Department of Internal Medicine, Nagasaki University School of Medicine, Japan.
Nakao
K
K
Sayama
K
K
Hamasaki
K
K
Kato
Y
Y
Nakata
K
K
Ishii
N
N
Butterfield
J H
JH
Galli
S J
SJ
D000001:Calcimycin / Q000494:pharmacology; D018197:Hepatoblastoma; D006801:Humans; D007946:Leukemia, Mast-Cell / Q000201:enzymology / Q000235:genetics / Q000378:metabolism*; D008113:Liver Neoplasms; D000090063:Proto-Oncogene Mas; D019859:Proto-Oncogene Proteins c-met / Q000096:biosynthesis* / Q000235:genetics / Q000494:pharmacology; D012333:RNA, Messenger / Q000096:biosynthesis / Q000187:drug effects; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology; D014158:Transcription, Genetic / Q000187:drug effects; D014407:Tumor Cells, Cultured; D014409:Tumor Necrosis Factor-alpha / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism
C000715470:MAS1 protein, human; D000090063:Proto-Oncogene Mas; D012333:RNA, Messenger; D014409:Tumor Necrosis Factor-alpha; D000001:Calcimycin; D019859:Proto-Oncogene Proteins c-met; D013755:Tetradecanoylphorbol Acetate