Multiplex DNA typing of short-tandem-repeat loci on the Y chromosome.
To facilitate evolutionary and forensic studies of DNA polymorphisms on the Y chromosome, we devised a multiplex amplification procedure for short-tandem-repeat (STR) loci. Four tetranucleotide STR loci (DYS19, DYS390, DYS391, and DYS393) were simultaneously amplified with FAM-labeled primers and genotypes were determined with an automated DNA sequencer. We typed 162 males from three U.S. populations (African-Americans, European-Americans and Hispanics) and found that the haplotype diversities range from 0.920 to 0.969. This quadruplex system provides a facile means of genotyping these Y chromosome STRs, and should be useful in population genetic and forensic applications.
Redd
A J
AJ
Department of Anthropology, Pennsylvania State University, University Park 16802, USA.
Clifford
S L
SL
Stoneking
M
M
D000328:Adult; D000483:Alleles; D004247:DNA / Q000737:chemistry* / Q000235:genetics*; D005838:Genotype; D006239:Haplotypes; D006801:Humans; D008297:Male; D018598:Minisatellite Repeats; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic; D014998:Y Chromosome / Q000737:chemistry*
D004247:DNA