Determination of urinary acylcarnitines by ESI-MS coupled with solid-phase microextraction (SPME).
The determination of selected short-, medium- and long-chain acylcarnitines by electrospray ionization mass spectrometry (ESI-MS) is discussed. The differences in fragmentation behaviour and ionization efficiency are described in dependence on collision induced dissociation (CID) conditions and mixture composition. A new method combination, solid-phase microextraction (SPME)-ESI-MS, is introduced to characterize acylcarnitines in body fluids. This method utilizes SPME for pre-concentration of acylcarnitines from complex biological samples and ESI-MS for a selective and sensitive detection. The method is presented by standard experiments determining of acylcarnitines in aqueous solutions and urine samples from patients with secondary carnitine deficiency syndromes or related disorders.
Möder
M
M
Centre for Environmental Research Leipzig-Halle Ltd, Department of Analytical Chemistry, Germany.
Löster
H
H
Herzschuh
R
R
Popp
P
P
D002138:Calibration; D002331:Carnitine / Q000031:analogs & derivatives* / Q000097:blood / Q000652:urine*; D002849:Chromatography, Gas; D002851:Chromatography, High Pressure Liquid; D006801:Humans; D013058:Mass Spectrometry; D012016:Reference Values
D002331:Carnitine