[Monocellular quantitative study on the adhesive ability of eosinophils in the course of eosinophils activation].
OBJECTIVE
To observe the dynamic changes of eosinophils (EOS) adhesive ability in the course of eosinophils activation.


METHODS
The adhesive ability EOS to vascular endothelial cell (EC) was measured by the use of a microminpulation technique.


RESULTS
The critical separation stress (Sc) between rest EOS and EC was 0.61 +/- 0.07kPa. Treatment of EOS with platelet activaty factor (PAF) caused a significant increase in Sc. The Sc was 4.1 +/- 0.6kPa within 30 minutes of EOS exposure to PAF, and had no tendency to increase continuously by exposure time prolonged.


CONCLUSIONS
The increase of EOS adhesive ability belongs to a kind of rapid response while EOS is activated. The increase could be an important basis of EOS adhesion to EC and EOS entry into bronchial mucosa.
Zhou
X
X
Department of Respiration, Dapin Hospital, Third Medical University, Chongqing.
Yang
Z
Z
Long
M
M
D002448:Cell Adhesion / Q000187:drug effects; D015550:Cell Degranulation / Q000187:drug effects; D004730:Endothelium, Vascular / Q000166:cytology*; D004804:Eosinophils / Q000502:physiology*; D006801:Humans; D008875:Middle Aged; D010972:Platelet Activating Factor / Q000494:pharmacology*
D010972:Platelet Activating Factor