Low frequency of autoantibodies against Ki-67 antigen in Japanese patients with systemic autoimmune diseases.
The Ki-67 antigen, which is recognized by the monoclonal antibody Ki-67, is a marker of cell proliferation. During cDNA cloning using sera from a patient with systemic lupus erythematosus, we obtained a positive clone encoding part of Ki-67. We determined the frequency of autoantibodies against Ki-67 in patients with systemic autoimmune diseases. Serum samples from 252 patients with rheumatic diseases were examined by immunoblotting with HeLa nuclear extract and the recombinant N-terminus of the Ki-67 antigen. Autoantibodies against Ki-67 antigen were detected in two out of 76 patients with systemic lupus erythematosus and one out of 90 patients with scleroderma. While, in a previous report, anti-Ki-67 antibodies were frequently targeted by a certain strain of autoimmune mice, our results indicated that Ki-67 was a minor target of autoantibodies among Japanese patients with systemic autoimmune diseases.
Muro
Y
Y
Department of Dermatology, Nagoya University School of Medicine, Nagoya, Japan. ymuro@tsuru.med.nagoya-u.ac.jp
Kano
T
T
Sugiura
K
K
Hagiwara
M
M
D001324:Autoantigens / Q000097:blood*; D001327:Autoimmune Diseases / Q000097:blood / Q000276:immunology*; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics / Q000378:metabolism; D006367:HeLa Cells; D006801:Humans; D015151:Immunoblotting; D019394:Ki-67 Antigen / Q000276:immunology*; D012216:Rheumatic Diseases / Q000097:blood
D001324:Autoantigens; D018076:DNA, Complementary; D019394:Ki-67 Antigen