title: Sequence and functional analysis of the gene encoding Vibrio cholerae cAMP receptor protein.
abstract: We describe here the cloning, nucleotide sequence, and functional expression of the crp gene of Vibrio cholerae (Vc) encoding the cyclic AMP receptor protein (CRP). The Vc crp gene shows 81% identity with the crp gene from Escherichia coli (Ec) and its deduced amino acid sequence shows 95% identity with the Ec protein. When expressed from inducible promoters, the cloned Vc gene produced an approximately 20-kDa protein which complemented the carbohydrate-negative and growth-defective phenotypes of both Ec and Vc crp mutants. In the Vc crp mutant, the cloned crp gene also restored the normal repression of ToxR-regulated virulence genes which occurs under certain environmental conditions.
authors.affiliation: Dartmouth Medical School, Department of Microbiology, Hanover, NH 03755, USA. karen.skorupski@dartmouth.edu
authors.forename: K
authors.initials: K
authors.lastname: Skorupski
authors.forename: R K
authors.initials: RK
authors.lastname: Taylor
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D002772:Cholera Toxin / Q000235:genetics; D003001:Cloning, Molecular; D004926:Escherichia coli / Q000235:genetics; D015964:Gene Expression Regulation, Bacterial; D005798:Genes, Bacterial; D008969:Molecular Sequence Data; D011953:Receptors, Cyclic AMP / Q000235:genetics*; D017386:Sequence Homology, Amino Acid; D012689:Sequence Homology, Nucleic Acid; D014734:Vibrio cholerae / Q000235:genetics*
chemical_list: D011953:Receptors, Cyclic AMP; D002772:Cholera Toxin