Priming and inducing effects of interleukin-3 on histamine release from cord-blood basophils.
The effect of interleukin-3 (IL-3) on histamine release from cord and adult blood basophils were evaluated. Leukocyte suspensions, obtained from adult patients with respiratory allergy (n = 15), normal adult subjects (n = 15), and neonates with (n = 15) and without (n = 19) atopic disposition, were stimulated with anti-IgE, fMLP, and IL-3. IgE-mediated histamine release was significantly higher in adult patients, either allergic or normal, than in neonates with or without atopic disposition. A trend toward higher fMLP-induced histamine release was found in allergic adult subjects. IL-3 had a weak direct histamine-releasing activity in allergic adult subjects and in neonates, but not in normal adult donors. A significant enhancing effect of IL-3 on histamine release induced by anti-IgE was observed in neonates with and without atopic disposition and in normal adult subjects, but not in atopic adult patients. IL-3 exerted a priming effect also when basophils were stimulated with fMLP, without any significant difference between neonates and adult subjects. Passive sensitization with IgE-rich serum resulted in a significant increase in anti-IgE-induced, but not in IL-3-induced, histamine release from cord-blood basophils. In conclusion, IL-3 primes cord-blood as well as adult blood basophils for a consecutive anti-IgE- or fMLP-induced histamine release and its activity is not limited by the low density of membrane IgE in cord-blood basophils.
Miadonna
A
A
Respiratory Allergy and Immunology Unit, IRCCS Ospedale Maggiore Policlinico, Milan, Italy.
Salmaso
C
C
Marco
M P
MP
Pugni
L
L
Milazzo
N
N
Tedeschi
A
A
Marini
A
A
D000328:Adult; D000888:Antibodies, Anti-Idiotypic / Q000276:immunology; D001491:Basophils / Q000276:immunology*; D005260:Female; D005312:Fetal Blood / Q000166:cytology; D006636:Histamine Release; D006801:Humans; D007116:Immunization, Passive; D007070:Immunoglobulin A / Q000032:analysis; D007073:Immunoglobulin E / Q000032:analysis / Q000276:immunology; D007231:Infant, Newborn; D007377:Interleukin-3 / Q000276:immunology*; D008297:Male; D009240:N-Formylmethionine Leucyl-Phenylalanine / Q000276:immunology; D012130:Respiratory Hypersensitivity
D000888:Antibodies, Anti-Idiotypic; D007070:Immunoglobulin A; D007377:Interleukin-3; D007073:Immunoglobulin E; D009240:N-Formylmethionine Leucyl-Phenylalanine