Transferrin--interactions of lactoferrin with hydrogen carbonate.
The interaction of apolactoferrin with hydrogen carbonate (bicarbonate) has been investigated in the pH range 6.5-9.2. In the absence of bicarbonate apolactoferrin loses a single proton with pK1a of 8.10. This proton loss is independent of the interaction with the synergistic anion. The C-site of apolactoferrin interacts with bicarbonate with a very low affinity (K(-1)C = 3.2 M(-1)). This process is accompanied by a proton loss, which is probably provided by the bicarbonate in interaction with the protein. This proton loss can possibly be the result of a shift in the proton dissociation constant, pKa, of the bicarbonate/carbonate acid/base equilibrium, which would decrease from pKa 10.35 to pK2a 6.90 in the bicarbonate-lactoferrin adduct. The N-site of the protein interacts with bicarbonate with an extremely low affinity, which excludes the presence of the N-site-synergistic anion adduct in neutral physiological media. Contrary to serum transferrin, the concentration of the apolactoferrin in interaction with bicarbonate is pH dependent. Between pH 7.4 and pH 9 with [HCO3-] about 20 mM, the concentration of the serum transferrin-bicarbonate adduct is always about 30%, whereas that of the apolactoferrin-synergistic anion adduct varies from 25% at pH 7.5 to 90% at pH 9. This implies that, despite an affinity for bicarbonate two orders of magnitude lower than that of serum transferrin, lactoferrin interacts better with the synergistic anion. This can be explained by the possible interaction of lactoferrin with carbonate in neutral media, whereas transferrin only interacts with bicarbonate.
Pakdaman
R
R
Institut de Topologie et de Dynamique des Systèmes de l'Université Paris 7, France.
El Hage Chahine
J M
JM
D000818:Animals; D001059:Apoproteins / Q000378:metabolism*; D001639:Bicarbonates / Q000378:metabolism*; D001665:Binding Sites; D001693:Biological Transport, Active; D002417:Cattle; D006863:Hydrogen-Ion Concentration; D066298:In Vitro Techniques; D007501:Iron / Q000378:metabolism; D007700:Kinetics; D007781:Lactoferrin / Q000378:metabolism*; D011485:Protein Binding; D011522:Protons; D014168:Transferrin / Q000378:metabolism*
D001059:Apoproteins; D001639:Bicarbonates; D011522:Protons; D014168:Transferrin; C039223:apolactoferrin; D007501:Iron; D007781:Lactoferrin