[Superoxide dismutase encapsulated erythrocytes used in the study of cerebral ischemia-reperfusion].
The possibility of using red blood cells (RBC) as the carrier of superoxide dismutase (SOD) was studied in this paper. SOD was encapsulated into rabbit erythrocytes (RBC-SOD), and was used in the study of cerebral ischemia-reperfusion (I-R). The effect of RBC-SOD on cerebral I-R was observed. The circulation half-life (T1/2) of RBC-SOD was determined in rabbits by 51Cr radiolabel. T1/2 was 13.4 +/- 1.5 days (n = 6) for RBC, and T1/2 was 13.7 +/- 1.0 days (n = 5) for RBC-SOD. The increase of lipid peroxidation (LPO) during cerebral I-R was inhibited by RBC-SOD. The maximum concentration of nitric oxide (NO) in plasma occurred in 10 minutes following reperfusion. The concentration of nitric oxide reduced gradually as reperfusion continued in both control and SOD groups, but it remained at a relatively high level continuous during the 4.5 hours of reperfusion in RBC-SOD group. These results indicate that intact RBC-SOD could scavenge superoxide anion (O2-.) produced during cerebral I-R.
Wang
X
X
Hematology Institute, CAMS, Tianjin.
Yang
J
J
Liu
F
F
D000818:Animals; D002545:Brain Ischemia / Q000378:metabolism*; D004337:Drug Carriers; D004912:Erythrocytes; D016166:Free Radical Scavengers / Q000008:administration & dosage / Q000494:pharmacology*; D008054:Lipid Peroxides / Q000378:metabolism; D009569:Nitric Oxide / Q000378:metabolism; D011817:Rabbits; D015427:Reperfusion Injury / Q000378:metabolism*; D013482:Superoxide Dismutase / Q000008:administration & dosage / Q000494:pharmacology*
D004337:Drug Carriers; D016166:Free Radical Scavengers; D008054:Lipid Peroxides; D009569:Nitric Oxide; D013482:Superoxide Dismutase