A new improved way of making double-barrelled ion-selective micro-electrodes.
The methods published for fabrication of double-barrelled ion-selective micro-electrodes are often difficult to follow and give inconsistent results. We describe here a new improved way of making double-barrelled ion-selective micro-electrodes from theta-style glass capillaries that offers several refinements which improve reproducibility: (1) good control of silanization and (2) dry-bevelling to assure no inter-barrel silane contamination and to minimize the signal-to-noise ratio by increasing the tip diameter, without compromising the sharpness of the tip.
Semb
S O
SO
Institute for Experimental Medical Research, University of Oslo, Ullevål Hospital, Norway.
Amundsen
B
B
Sejersted
O M
OM
D000818:Animals; D004867:Equipment Design; D017736:Ion-Selective Electrodes; D008839:Microelectrodes; D008855:Microscopy, Electron, Scanning; D018485:Muscle Fibers, Skeletal / Q000378:metabolism; D009206:Myocardium / Q000166:cytology; D012756:Sheep; D012821:Silanes; D012964:Sodium / Q000493:pharmacokinetics
D012821:Silanes; D012964:Sodium