Selection of a strain of Clostridium argentinense producing high titers of type G botulinal toxin.
The strain G89HT of Clostridium argentinense obtained by culture selection of the prototype G89 strain producing high titers of type G botulinal toxin was studied. Its cultural, biochemical and toxigenic characteristics and the presence of plasmids were tested. Both strains showed similar physiological features and carried a 83 MDa plasmid. A 170 MDa plasmid was also recognized in the G89HT strain. Notably, this strain was better sporulating and showed a higher toxigenicity than the prototype G89 C. argentinense strain. These two characteristics might permit a long term storage and perhaps yield high antitoxin titres.
Puig de Centorbi
O
O
Area de Microbiología, Universidad Nacional de San Luis, Argentina.
Quiroz
H M
HM
Bogni
C
C
Calzolari
A
A
Centorbi
H J
HJ
D001426:Bacterial Proteins / Q000032:analysis; D001905:Botulinum Toxins / Q000378:metabolism*; D003013:Clostridium / Q000254:growth & development / Q000378:metabolism* / Q000472:pathogenicity; D003470:Culture Media / Q000378:metabolism; D009500:Neutralization Tests; D010957:Plasmids / Q000235:genetics / Q000302:isolation & purification; D013171:Spores, Bacterial / Q000235:genetics / Q000254:growth & development / Q000378:metabolism; D014357:Trypsin / Q000378:metabolism; D014774:Virulence / Q000235:genetics
D001426:Bacterial Proteins; D003470:Culture Media; D014357:Trypsin; D001905:Botulinum Toxins