title: Trp64Arg substitution in the beta 3-adrenergic receptor does not relate to body weight in healthy, premenopausal women.
abstract: OBJECTIVE
  To examine the contribution of the substitution of arginine for tryptophan at amino acid position 64 (Trp64Arg) of the beta 3-adrenergic receptor to body weight and weight-related factors in healthy, premenopausal women.
  
  
  METHODS
  Five hundred and five healthy, premenopausal women 44-50 y, body mass index (BMI) 20-40 kg/m2.
  
  
  METHODS
  Subjects were genotyped for the Trp64Arg substitution using restriction fragment length polymorphism (RFLP) analysis. Measurements for weight and weight-related factors (for example, weight, waist-hip ratio, lipid levels) were also taken.
  
  
  RESULTS
  Two women were homozygous Arg/Arg, 75 women were heterozygous Trp/Arg, and 428 women were homozygous Trp/Trp. The frequency of the Arg substitution was 0.078. When subjects with and without the arginine substitution were compared, no significant differences were found on measures of weight or weight-related risk factors.
  
  
  CONCLUSIONS
  The results confirm previous studies suggesting that the Trp64Arg substitution of the beta 3-adrenergic receptor is not related to weight or weight-related phenotypes in healthy, premenopausal women.
authors.affiliation: Department of Human Genetics, University of Pittsburgh, Graduate School of Public Health, PA 15213, USA.
authors.forename: M
authors.initials: M
authors.lastname: Moriarty
authors.forename: R R
authors.initials: RR
authors.lastname: Wing
authors.forename: L H
authors.initials: LH
authors.lastname: Kuller
authors.forename: R E
authors.initials: RE
authors.lastname: Ferrell
mesh_terms: D000483:Alleles; D000886:Anthropometry; D001483:Base Sequence; D001835:Body Weight / Q000235:genetics; D017931:DNA Primers / Q000737:chemistry; D003924:Diabetes Mellitus, Type 2 / Q000208:ethnology / Q000235:genetics; D005260:Female; D005787:Gene Frequency; D005838:Genotype; D006579:Heterozygote; D006720:Homozygote; D006801:Humans; D008875:Middle Aged; D009765:Obesity / Q000208:ethnology / Q000235:genetics*; D017354:Point Mutation / Q000235:genetics*; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length; D017697:Premenopause / Q000235:genetics*; D011943:Receptors, Adrenergic, beta / Q000737:chemistry / Q000235:genetics*; D022702:Receptors, Adrenergic, beta-3; D014364:Tryptophan / Q000235:genetics
chemical_list: D017931:DNA Primers; D011943:Receptors, Adrenergic, beta; D022702:Receptors, Adrenergic, beta-3; D014364:Tryptophan