DNA binding studies with cis-dichlorobis (5(6)-non/chlorosubstituted-2-hydroxymethyl-benzimidazole) platinum(II) complexes.
The DNA binding properties of two new platinum compounds, cis-[Pt(L)2Cl2]. 2H2O where L is 5(6)-non/chlorosubstituted-2-hydroxymethylbenzimidazole, were examined and compared with cisplatin. The platinum compounds (compounds I and II) were used to modify DNA, which was then used in electrophoretic mobility shift assays with the high mobility group (HMG)-do-main protein, HMG1. The DNA platinated with these compounds was specifically recognized by HMG1. It was concluded that the adducts formed by compounds I and II distort the DNA in a manner similar to cisplatin diadducts.
Gümüş
F
F
Department of Pharmaceutical Chemistry, School of Pharmacy, Gazi University, Etiler-Ankara, Turkey.
Algül
O
O
D001562:Benzimidazoles / Q000737:chemistry / Q000378:metabolism*; D002352:Carrier Proteins / Q000378:metabolism; D002945:Cisplatin / Q000737:chemistry / Q000378:metabolism; D004247:DNA / Q000737:chemistry / Q000378:metabolism*; D018736:DNA Adducts / Q000737:chemistry / Q000378:metabolism; D004586:Electrophoresis / Q000379:methods; D024243:HMGB1 Protein; D006609:High Mobility Group Proteins / Q000378:metabolism; D009944:Organoplatinum Compounds / Q000737:chemistry / Q000378:metabolism*
D001562:Benzimidazoles; D002352:Carrier Proteins; D018736:DNA Adducts; D024243:HMGB1 Protein; D006609:High Mobility Group Proteins; D009944:Organoplatinum Compounds; C108658:dichlorobis(2-hydroxymethylbenzimidazole)platinum(II); C108659:dichlorobis(5-chloro-2-hydroxymethylbenzimidazole)platinum(II); C108660:dichlorobis(6-chloro-2-hydroxymethylbenzimidazole)platinum(II); D004247:DNA; D002945:Cisplatin