title: Identification of yebG as a DNA damage-inducible Escherichia coli gene.
abstract: The nucleotide sequence upstream of the Escherichia coli yebG gene presents features similar to those found in SOS system regulatory sites (putative SOS box, -10 and -35 promoter boxes and a ribosome binding site). Operon fusion assays demonstrate now that this region controls transcription in a recA-, lexA-dependent way and that the reporter gene expression is inducible by DNA damage consequent to mitomycin C treatment. Increased expression does not result from an increase in plasmid copy number. These results indicate that yebG is a novel SOS regulon gene. The yebG product is predicted to be a 96 amino acid residue, 10.7 kDa protein whose function is not yet known. Unlike other SOS genes, the construct carrying the yebG regulatory region is not stationary phase inducible.
authors.affiliation: Instituto de Biofisica Carlos Chagas Filho, Universidade Federal do Rio de Janeiro, Brazil. darcy@lncc.br
authors.forename: M R
authors.initials: MR
authors.lastname: Lomba
authors.forename: A T
authors.initials: AT
authors.lastname: Vasconcelos
authors.forename: A B
authors.initials: AB
authors.lastname: Pacheco
authors.forename: D F
authors.initials: DF
authors.lastname: de Almeida
mesh_terms: D001483:Base Sequence; D016384:Consensus Sequence; D004249:DNA Damage / Q000235:genetics*; D004269:DNA, Bacterial / Q000235:genetics; D004926:Escherichia coli / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism; D005798:Genes, Bacterial; D008969:Molecular Sequence Data; D009876:Operon; D018085:Regulon; D013014:SOS Response, Genetics / Q000235:genetics
chemical_list: D004269:DNA, Bacterial