Cloning, sequencing and regulation of thiA, a thiamin biosynthesis gene from Bacillus subtilis.
The thiA gene is involved in the biosynthesis of the pyrimidine moiety of thiamin in Bacillus subtilis. This gene was cloned using a DNA probe rescued from thiA::Tn917 to screen a B. subtilis genomic library. ThiA exhibits 70% identity with E. coli thiC. The proposed thiA open reading frame complements all available thiA mutants of B. subtilis as well as the thiC mutant of E. coli. This suggests a similar biosynthetic pathway to 2-methyl-4-amino-5-hydroxymethylpyrimidine in both organisms. The expression of thiA is regulated, at least on the transcriptional level, and is significantly repressed by thiamin and 2-methyl-4-amino-5-hydroxymethylpyrimidine.
Zhang
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Department of Chemistry, Cornell University, Ithaca, NY 14853-1380, USA.
Begley
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D000595:Amino Acid Sequence; D001412:Bacillus subtilis / Q000235:genetics*; D001426:Bacterial Proteins / Q000235:genetics*; D001483:Base Sequence; D003001:Cloning, Molecular; D004269:DNA, Bacterial / Q000235:genetics; D004926:Escherichia coli / Q000235:genetics; D005798:Genes, Bacterial; D005816:Genetic Complementation Test; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D011743:Pyrimidines / Q000096:biosynthesis; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid; D013831:Thiamine / Q000096:biosynthesis*
D001426:Bacterial Proteins; D004269:DNA, Bacterial; D011743:Pyrimidines; C109094:ThiA protein, Bacillus subtilis; D013831:Thiamine