Protection against verocytotoxin in mice induced by liposome-coupled verocytotoxin.
Purified verocytotoxins (VTs), VT1 and VT2, were coupled to liposomes via glutaraldehyde. During the coupling procedure, both VT1 and VT2 were detoxified. Intraperitoneal injection in BALB/c mice with either VT1-liposome or VT2-liposome induced a substantial amount of anti-VT1 or anti-VT2 IgG antibody production, respectively. Mice immunized with VT2-liposome were protected against intravenous challenge with a lethal dose of VT2 and the degree of protection correlated well with the amount of IgG induced against VT2. Although VT1-liposome failed to induce protection against VT1, the decrease of the body weight observed after the toxin challenge correlated inversely with the amount of anti-VT1 IgG induced, suggesting that VT1 neutralizing antibody was present in VT1-liposome-immune mice. In addition, VT-liposome conjugate induced no detectable anti-VT IgE antibody production. These results demonstrate the potential ability of VT-liposome conjugates for the production of VT vaccine which induces protection against VTs.
Naito
S
S
Department of Safety Research on Biologics, National Institute of Infectious Diseases, Musashimurayama, Tokyo, Japan.
Horino
A
A
Komiya
T
T
Fukuda
Y
Y
Takahashi
M
M
Ami
Y
Y
Suzaki
Y
Y
Satoh
S
S
Gondaira
F
F
Sugiyama
J
J
Nakano
Y
Y
Mori
M
M
Awai
K
K
Nishinohara
S
S
Komuro
K
K
Uchida
T
T
D000818:Animals; D000907:Antibodies, Bacterial / Q000096:biosynthesis; D001427:Bacterial Toxins / Q000276:immunology*; D001428:Bacterial Vaccines / Q000008:administration & dosage; D003603:Cytotoxins / Q000276:immunology*; D004926:Escherichia coli; D004927:Escherichia coli Infections / Q000209:etiology / Q000276:immunology / Q000517:prevention & control*; D005260:Female; D018796:Immunoconjugates / Q000008:administration & dosage*; D007073:Immunoglobulin E / Q000096:biosynthesis; D007074:Immunoglobulin G / Q000096:biosynthesis; D008081:Liposomes / Q000276:immunology*; D051379:Mice; D008807:Mice, Inbred BALB C; D022622:Shiga Toxin 1
D000907:Antibodies, Bacterial; D001427:Bacterial Toxins; D001428:Bacterial Vaccines; D003603:Cytotoxins; D018796:Immunoconjugates; D007074:Immunoglobulin G; D008081:Liposomes; D022622:Shiga Toxin 1; D007073:Immunoglobulin E