Extreme heat- and pressure-resistant 7-kDa protein P2 from the archaeon Sulfolobus solfataricus is dramatically destabilized by a single-point amino acid substitution.
This study reports the characterization of the recombinant 7-kDa protein P2 from Sulfolobus solfataricus and the mutants F31A and F31Y with respect to temperature and pressure stability. As observed in the NMR, FTIR, and CD spectra, wild-type protein and mutants showed substantially similar structures under ambient conditions. However, midpoint transition temperatures of the denaturation process were 361, 334, and 347 K for wild type, F31A, and F31Y mutants, respectively: thus, alanine substitution of phenylalanine destabilized the protein by as much as 27 K. Midpoint transition pressures for wild type and F31Y mutant could not be accurately determined because they lay either beyond (wild type) or close to (F31Y) 14 kbar, a pressure at which water undergoes a phase transition. However, a midpoint transition pressure of 4 kbar could be determined for the F31A mutant, implying a shift in transition of at least 10 kbar. The pressure-induced denaturation was fully reversible; in contrast, thermal denaturation of wild type and mutants was only partially reversible. To our knowledge, both the pressure resistance of protein P2 and the dramatic pressure and temperature destabilization of the F31A mutant are unprecedented. These properties may be largely accounted for by the role of an aromatic cluster where Phe31 is found at the core, because interactions among aromatics are believed to be almost pressure insensitive; furthermore, the alanine substitution of phenylalanine should create a cavity with increased compressibility and flexibility, which also involves an impaired pressure and temperature resistance.
Fusi
P
P
Dipartimento di Fisiologia e Biochimica generali, Università di Milano, Italy.
Goossens
K
K
Consonni
R
R
Grisa
M
M
Puricelli
P
P
Vecchio
G
G
Vanoni
M
M
Zetta
L
L
Heremans
K
K
Tortora
P
P
D000409:Alanine; D019943:Amino Acid Substitution; D019843:Archaeal Proteins / Q000737:chemistry / Q000235:genetics / Q000502:physiology; D004268:DNA-Binding Proteins / Q000737:chemistry / Q000235:genetics / Q000502:physiology*; D006358:Hot Temperature; D010649:Phenylalanine; D017354:Point Mutation; D011312:Pressure; D017510:Protein Folding; D011993:Recombinant Fusion Proteins / Q000737:chemistry / Q000235:genetics / Q000502:physiology; D012260:Ribonucleases / Q000737:chemistry / Q000235:genetics / Q000502:physiology*; D016944:Sulfolobus / Q000201:enzymology*
D019843:Archaeal Proteins; D004268:DNA-Binding Proteins; C110356:P2 protein, Sulfolobus; D011993:Recombinant Fusion Proteins; D010649:Phenylalanine; D012260:Ribonucleases; D000409:Alanine