Detection of staphylococcal enterotoxin B employing a piezoelectric crystal immunosensor.
This paper reports on a study concerning the development of a biochemical sensor for Staphylococcal Enterotoxin B (SEB), a substance within the bracket of biological warfare. A 20 MHz piezoelectric quartz crystal sensor device was employed in a flow injection system. The assay for SEB is based on a competition scheme using polyclonal antibodies (anti-SEB). Three parameters, i.e. the flow rate in the flow cell, the incubation time and the anti-SEB concentration, were optimized. A detection limit of 0.1 microgram/ml was obtained, whereas at concentrations of SEB of 10 micrograms/ml or higher the sensor response was completely inhibited. The results were compared with a competition enzyme linked immunosorbent assay.
Harteveld
J L
JL
TNO Prins Maurits Laboratory, Rijswijk, The Netherlands.
Nieuwenhuizen
M S
MS
Wils
E R
ER
D001694:Biological Warfare; D015374:Biosensing Techniques; D003460:Crystallization; D004563:Electrochemistry / Q000295:instrumentation*; D004768:Enterotoxins / Q000032:analysis*; D004797:Enzyme-Linked Immunosorbent Assay; D012212:Rheology; D013211:Staphylococcus aureus
D004768:Enterotoxins; C031818:enterotoxin B, staphylococcal