title: Cytochrome P-450 reductase is responsible for the ferrireductase activity associated with isolated plasma membranes of Saccharomyces cerevisiae.
abstract: Cytochrome P-450 reductase (encoded by the NCP1 gene) was found to catalyse all the NADPH-dependent ferrireductase activities associated with isolated plasma membranes of the yeast Saccharomyces cerevisiae. We therefore examined the contribution of this enzyme to the ferrireductase activity of cells in vivo. Cytochrome P-450 reductase was shown to be not essential for the cell ferrireductase activity, but it influenced this activity, with different effects on the Fre1- and the Fre2-dependent reductase systems. Overexpression of FRE1 did not lead to an increased ferrireductase activity of the cells when NCP1 was repressed. In contrast, cells that overexpressed FRE2 had maximal ferrireductase activity when NCP1 was repressed. The degree of NCP1 expression also affected the amount of iron and copper accumulated by the cells during growth. The biochemical implications and the physiological significance of these observations are discussed.
authors.affiliation: Laboratoire de Biochimie des Porphyrines, Institut Jacques-Monod, Université Paris, France. lesuisse@ijm.jussieu.fr
authors.forename: E
authors.initials: E
authors.lastname: Lesuisse
authors.forename: M
authors.initials: M
authors.lastname: Casteras-Simon
authors.forename: P
authors.initials: P
authors.lastname: Labbe
mesh_terms: D001693:Biological Transport, Active; D002462:Cell Membrane / Q000201:enzymology; D003300:Copper / Q000378:metabolism; D038181:FMN Reductase; D015870:Gene Expression; D005800:Genes, Fungal; D007501:Iron / Q000378:metabolism; D009247:NADH, NADPH Oxidoreductases / Q000235:genetics / Q000378:metabolism*; D009251:NADPH-Ferrihemoprotein Reductase / Q000235:genetics / Q000378:metabolism*; D012441:Saccharomyces cerevisiae / Q000201:enzymology* / Q000235:genetics / Q000254:growth & development
chemical_list: D003300:Copper; D007501:Iron; D038181:FMN Reductase; D009247:NADH, NADPH Oxidoreductases; D009251:NADPH-Ferrihemoprotein Reductase; C023903:ferric citrate iron reductase