Suppressing effects of S-methyl methanethiosulfonate and diphenyl disulfide on mitomycin C-induced somatic mutation and recombination in Drosophila melanogaster and micronuclei in mice.
S-Methyl methanethiosulfonate (MMTS) and diphenyl disulfide (DPDS) are temporary enzyme-sulfhydryl blocking agents. They are naturally occurring phytoalexin-like and synthetic substances known to be very potent bio-antimutagens in Escherichia coli B/r WP2. In the present paper, the suppressing effects of MMTS on mitomycin C (MMC)-induced mutant wing spots in the somatic mutation and recombination test (SMART) of Drosophila melanogaster, and of MMTS and DPDS on MMC-induced micronucleated peripheral reticulocytes are described. MMTS consistently reduced the numbers of MMC-induced small single, large single and twin spots per wing at a dose of 10-1000 micrograms/vial, in a dose-dependent manner. MMTS reduced the number of twin spots per wing on the spontaneous mutation at the dose of 1000 micrograms/vial. MMTS and DPDS dose-dependently reduced the frequencies of MMC-induced micronucleated peripheral reticulocytes at a dose of 10-40, and 3-100 micrograms/kg, respectively. Our results confirmed that enzyme-sulfhydryl blocking agents, such as MMTS and DPDS, are effective antimutagens in vivo too.
Nakamura
Y K
YK
Laboratory of Health Science, School of Pharmaceutical Sciences, University of Shizuoka, Japan.
Kawai
K
K
Furukawa
H
H
Matsuo
T
T
Shimoi
K
K
Tomita
I
I
Nakamura
Y
Y
D000818:Animals; D016587:Antimutagenic Agents / Q000494:pharmacology*; D004220:Disulfides / Q000494:pharmacology*; D004331:Drosophila melanogaster / Q000235:genetics; D008297:Male; D008741:Methyl Methanesulfonate / Q000031:analogs & derivatives* / Q000494:pharmacology; D051379:Mice; D008813:Mice, Inbred ICR; D015162:Micronucleus Tests; D016685:Mitomycin / Q000494:pharmacology; D009153:Mutagens / Q000494:pharmacology; D009154:Mutation / Q000187:drug effects*; D011995:Recombination, Genetic / Q000187:drug effects*; D012156:Reticulocytes; D014921:Wings, Animal
D016587:Antimutagenic Agents; D004220:Disulfides; D009153:Mutagens; C014674:methyl methanethiosulfonate; D016685:Mitomycin; C053871:diphenyl disulfide; D008741:Methyl Methanesulfonate