title: Targeting of HIV- and SIV-infected cells by CD4-chemokine receptor pseudotypes.
abstract: Retroviral vectors containing CD4 and an appropriate chemokine receptor were evaluated for the ability to transduce cells infected with human immunodeficiency virus (HIV) and simian immunodeficiency virus (SIV). These CD4-chemokine receptor pseudotypes were able to target HIV- and SIV-infected cell lines and monocyte-derived macrophages in a manner that corresponded to the specificity of the viral envelope glycoprotein for its CD4-chemokine receptor complex. This approach could offer a way to deliver antiviral genes directly to HIV-infected cells in vivo and could provide an additional treatment strategy in conjunction with existing antiviral therapies.
authors.affiliation: Department of Medicine, Hematology-Oncology Division, University of Pennsylvania, Philadelphia, PA 19104, USA. endres@mail.med.upenn.edu
authors.forename: M J
authors.initials: MJ
authors.lastname: Endres
authors.forename: S
authors.initials: S
authors.lastname: Jaffer
authors.forename: B
authors.initials: B
authors.lastname: Haggarty
authors.forename: J D
authors.initials: JD
authors.lastname: Turner
authors.forename: B J
authors.initials: BJ
authors.lastname: Doranz
authors.forename: P J
authors.initials: PJ
authors.lastname: O'Brien
authors.forename: D L
authors.initials: DL
authors.lastname: Kolson
authors.forename: J A
authors.initials: JA
authors.lastname: Hoxie
mesh_terms: D000818:Animals; D015704:CD4 Antigens / Q000235:genetics* / Q000378:metabolism; D002460:Cell Line; D015686:Gene Products, env / Q000378:metabolism; D018014:Gene Transfer Techniques; D005822:Genetic Vectors; D015497:HIV-1 / Q000502:physiology*; D006801:Humans; D008264:Macrophages / Q000821:virology; D010957:Plasmids; D019713:Receptors, CCR5 / Q000235:genetics / Q000378:metabolism; D019718:Receptors, CXCR4 / Q000235:genetics / Q000378:metabolism; D019707:Receptors, Chemokine / Q000235:genetics* / Q000378:metabolism; D015302:Simian Immunodeficiency Virus / Q000502:physiology*; D014162:Transfection
chemical_list: D015704:CD4 Antigens; D015686:Gene Products, env; D019713:Receptors, CCR5; D019718:Receptors, CXCR4; D019707:Receptors, Chemokine