Elevation of apolipoprotein E in the CSF of cattle affected by BSE.
The cerebrospinal fluid (CSF) of patients suffering from Creutzfeldt-Jakob disease (CJD) display two unique polypeptide chains by two-dimensional polyacrylamide gel electrophoresis (2-D PAGE). In the absence of a well-defined ante-mortem diagnostic test for bovine spongiform encephalopathy (BSE), spinal fluid samples of eight normal cows and eight cows known to carry BSE by post-mortem histological analysis were investigated to verify if equivalent polypeptides were present. Proteins with similar migration to human CJD polypeptides were not detected. But surprisingly, a cluster of polypeptide spots that was faint or not detected in normal bovine CSF samples was found to be elevated or massively increased in BSE CSF samples (more than 10-fold increase). These elevated polypeptide chains were identified as apolipoprotein E.
Hochstrasser
D F
DF
Clinical Chemistry Laboratory, Geneva University Hospital (HUG), Switzerland.
Frutiger
S
S
Wilkins
M R
MR
Hughes
G
G
Sanchez
J C
JC
D000595:Amino Acid Sequence; D000818:Animals; D001057:Apolipoproteins E / Q000134:cerebrospinal fluid* / Q000737:chemistry; D002417:Cattle; D007562:Creutzfeldt-Jakob Syndrome / Q000134:cerebrospinal fluid*; D015180:Electrophoresis, Gel, Two-Dimensional; D004591:Electrophoresis, Polyacrylamide Gel; D016643:Encephalopathy, Bovine Spongiform / Q000134:cerebrospinal fluid*; D006801:Humans; D008969:Molecular Sequence Data; D010446:Peptide Fragments / Q000737:chemistry; D012016:Reference Values; D015203:Reproducibility of Results; D013045:Species Specificity
D001057:Apolipoproteins E; D010446:Peptide Fragments