[Effects of gossypol acetic acid on human ejaculated sperm Ca2+ influx and rat vas deferens contraction action].
It has been reported that gossypol acetic acid (GAA) inhibits sperm motility and calcium plays an important role in regulating the function of the sperm flagella. In order to explore the mechanism by which GAA inhibits sperm motility, the effects of GAA on human ejaculated sperm Ca2+ influx and isolated rat vas deferens contraction action were studied. The results showed that GAA inhibited sperm Ca2+ influx in a dose-dependent manner, and breaked the dynamic equilibrium of sperm internal and external Ca2+ gradient concentrations. It may be one of important mechanism by which GAA inhibits sperm motility. But GAA exerts no effect on the high K+ induced contraction of smooth muscle of isolated rat vas deferens. It is likely that GAA has selective action on the sperm membrane of the rats.
Wu
T
T
National Research Institute for Family Planning, Beijing.
Sha
Y
Y
Han
F
F
Duan
J
J
Cheng
J
J
D000328:Adult; D000818:Animals; D001693:Biological Transport, Active / Q000187:drug effects; D002118:Calcium / Q000378:metabolism*; D004305:Dose-Response Relationship, Drug; D006072:Gossypol / Q000031:analogs & derivatives* / Q000494:pharmacology; D006801:Humans; D008297:Male; D009119:Muscle Contraction / Q000187:drug effects*; D051381:Rats; D017207:Rats, Sprague-Dawley; D013089:Spermatocidal Agents / Q000494:pharmacology*; D013094:Spermatozoa / Q000378:metabolism; D014649:Vas Deferens / Q000187:drug effects*
D013089:Spermatocidal Agents; D006072:Gossypol; C028178:gossypol acetic acid; D002118:Calcium