Location, immunohistochemical features, and spinal connections of autonomic neurons innervating the rat seminal vesicles.
With the use of retrograde tracing techniques, selective spinal nerve transections, and immunohistochemistry to label noradrenergic and peptidergic pathways, this study has for the first time defined in detail the autonomic innervation to the rat seminal vesicles. The majority of this innervation originates from the bilateral major pelvic ganglia, whereas very few neurons are located in the accessory, inferior mesenteric, or paravertebral chain ganglia. Neuropeptide Y was the most abundant marker, followed by tyrosine hydroxylase (an enzyme involved in noradrenaline synthesis), and then vasoactive intestinal peptide. Sympathetic axons with tyrosine hydroxylase and neuropeptide Y supplied vascular and nonvascular smooth muscle whereas parasympathetic, cholinergic neuropeptide Y terminals were associated with the glandular epithelium. In contrast, vasoactive intestinal peptide was found only in cholinergic neurons, which may have either parasympathetic or sympathetic spinal connections. The latter were far more prevalent, demonstrating a substantial sympathetic cholinergic innervation to the seminal vesicles. Vasoactive intestinal peptide axons were associated with the glandular epithelia, as well as vascular and nonvascular smooth muscle. Axons associated with the secretory epithelia may regulate secretion or perhaps provide trophic support. Finally, acute damage to preganglionic sacral and lumbar nerves caused a transient increase in glandular weight.
Kepper
M E
ME
Department of Physiology and Pharmacology, The University of Queensland, St. Lucia, Australia. kepper@plpk.uq.edu.au
Keast
J R
JR
D000818:Animals; D001339:Autonomic Fibers, Preganglionic / Q000502:physiology; D001341:Autonomic Nervous System / Q000166:cytology / Q000502:physiology*; D017776:Autonomic Pathways / Q000166:cytology / Q000502:physiology; D005456:Fluorescent Dyes; D007150:Immunohistochemistry; D008297:Male; D009474:Neurons / Q000502:physiology*; D009478:Neuropeptide Y / Q000378:metabolism; D009929:Organ Size; D017729:Presynaptic Terminals / Q000502:physiology; D051381:Rats; D017208:Rats, Wistar; D012669:Seminal Vesicles / Q000294:innervation*; D013116:Spinal Cord / Q000166:cytology / Q000502:physiology*; D013266:Stilbamidines; D014660:Vasoactive Intestinal Peptide / Q000378:metabolism
C049774:2-hydroxy-4,4'-diamidinostilbene, methanesulfonate salt; D005456:Fluorescent Dyes; D009478:Neuropeptide Y; D013266:Stilbamidines; D014660:Vasoactive Intestinal Peptide