Enhancement of 5-fluorouracil cytotoxicity by folinic acid in different cell-lines of human renal cell carcinoma.
Results of cytotoxic chemotherapy in metastatic renal cell carcinoma are not impressive. Remission rates range between 0 and 20%. One of the substances which show a marginal effect is 5-fluorouracil (5-FU). The cytotoxicity of 5-FU can be modulated by combination with folinic acid as shown in various cell lines and clinical trials. We were interested to see whether such a biomodulation also occurs in renal cell cancer. The antiproliferative effect of 5-fluorouracil on two human cell lines of RCC and its potentiation by folinic acid was investigated in a monolayer proliferation assay. It could be shown that folinic acid enhanced the cytotoxic potential of 5-FU 6-8-fold. Our results indicate that the combination of these two drugs in the treatment of metastatic renal cell cancer might lead to better response rates.
Breul
J
J
Urologische Klinik und Poliklinik der Technischen Universität M-unchen, Klinikum rechts der Isar Ismaningerstr. 22, Munich D-81675, Germany.
Tief
G
G
Hartung
R
R
D002292:Carcinoma, Renal Cell; D002455:Cell Division / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D004357:Drug Synergism; D005472:Fluorouracil / Q000633:toxicity*; D006801:Humans; D007680:Kidney Neoplasms; D002955:Leucovorin / Q000633:toxicity*; D014407:Tumor Cells, Cultured
D002955:Leucovorin; D005472:Fluorouracil