title: Occurrence of the same peroxidative compounds in low density lipoprotein and in atherosclerotic lesions from a homozygous familial hypercholesterolemic patient: a case report.
abstract: Oxidative modification of low density lipoprotein (LDL) and its byproducts may play a fundamental role in atherosclerosis. We report an in vitro analysis of LDL peroxidative compounds in an homozygous familial hypercholesterolemic (HFH) patient who subsequently died. During the autopsy, we analyzed lipids extracted directly from different atherosclerotic plaques, and we also provided an immunocytochemical analysis using the specific monoclonal antibody MDA2 (directed against malondialdeyde-lysine epitopes of oxidized LDL). The results showed that the same species of peroxidative compounds were present both in LDL in vitro and in lipids extracted directly from atherosclerotic lesions. Moreover, the immunocytochemistry analysis revealed a positive staining of atherosclerotic plaques, confirming the presence of LDL oxidation-specific epitopes. Although observation of a single case is necessarily limited, our findings are consistent with the hypothesis that oxidative modification of LDL is involved in human atherogenesis.
authors.affiliation: Division of Cardiology, School of Medicine, Federico II University of Naples, Italy.
authors.forename: C
authors.initials: C
authors.lastname: Napoli
authors.forename: F P
authors.initials: FP
authors.lastname: D'Armiento
authors.forename: G
authors.initials: G
authors.lastname: Corso
authors.forename: G
authors.initials: G
authors.lastname: Ambrosio
authors.forename: G
authors.initials: G
authors.lastname: Palumbo
authors.forename: P
authors.initials: P
authors.lastname: Zuliani
authors.forename: A
authors.initials: A
authors.lastname: Malorni
authors.forename: G
authors.initials: G
authors.lastname: Gallotta
authors.forename: P
authors.initials: P
authors.lastname: Somma
authors.forename: A
authors.initials: A
authors.lastname: Postiglione
authors.forename: M
authors.initials: M
authors.lastname: Chiariello
mesh_terms: D000328:Adult; D001161:Arteriosclerosis / Q000378:metabolism* / Q000473:pathology; D001344:Autopsy; D008078:Cholesterol, LDL / Q000097:blood*; D046508:Culture Techniques; D017809:Fatal Outcome; D005260:Female; D005609:Free Radicals / Q000378:metabolism; D006801:Humans; D006938:Hyperlipoproteinemia Type II / Q000378:metabolism* / Q000473:pathology; D007150:Immunohistochemistry; D015227:Lipid Peroxidation
chemical_list: D008078:Cholesterol, LDL; D005609:Free Radicals