[The experimental study of expression of IL-2 cDNA in human bladder tumor cell and its biological behaviors].
We report the significance of expression of interleukin-2 (IL-2) in human bladder tumor cell T24-transfected with IL-2 cDNA and its effects an biological behavior of the cell. The positive clone of T24/IL-2 and T24/neo were selected by G418 after transfection using calcium phosphate precipitation method. By hybridization in situ, immunocytochemistry, and biological activity assays, we found that IL-2 cDNA was successfuly delivered into T24 cell. The activity of IL-2 in T24/IL-2 cell culture medium was about 8-32IU/1 x 10(5) cell/24 h during the observation period of about 40 days. There were no marked differences in growing curve and clonegenicity in soft agar medium among T24, T24/neo and T24/IL-2. But the cell volume of T24/IL-2 was smaller in soft agar, and the tumorigenicity of T24/IL-2 was decreased in nude mouse.
Jin
F
F
Department of Urology, Daping Hospital, Third Military Medical University, Chongqing.
Yu
M
M
Hui
H
H
D000818:Animals; D018076:DNA, Complementary / Q000235:genetics; D015316:Genetic Therapy; D006801:Humans; D007376:Interleukin-2 / Q000096:biosynthesis* / Q000235:genetics; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D009368:Neoplasm Transplantation; D014162:Transfection; D014407:Tumor Cells, Cultured / Q000378:metabolism; D001749:Urinary Bladder Neoplasms / Q000235:genetics* / Q000473:pathology / Q000628:therapy
D018076:DNA, Complementary; D007376:Interleukin-2