Comparative genotoxicity of halogenated acetic acids found in drinking water.
Three short-term assays (SOS chromotest, Ames fluctuation test and newt micronucleus test) were performed to detect the genotoxic activity of organohalides, compounds likely to be found in chlorinated and/or ozonated drinking water: monochloro-, dichloro- and trichloroacetic acids and monobromo-, dibromo- and tribromoacetic acids. With the SOS chromotest, only three of the chemicals studied (dichloroacetic acid, dibromo- and tribromoacetic acids) were found to induce primary DNA damage in Escherichia coli PQ 37. In the Ames fluctuation test, all the compounds except monochloroacetic acid showed mutagenic activity in Salmonella typhimurium strain TA100. In these two in vitro tests, a good correlation between increasing number of substituents and decreasing mutagenicity was observed. Namely, the toxicity of brominated and chlorinated acetic acids decreased when the number of substituents increased. The newt micronucleus test detected a weak clastogenic effect on the peripheral blood erythrocytes of Pleurodeles waltl larvae for trichloroacetic acid only.
Giller
S
S
Laboratory of Genetic Toxicology, Pasteur Institute of Lille, France.
Le Curieux
F
F
Erb
F
F
Marzin
D
D
D000085:Acetates / Q000633:toxicity*; D000818:Animals; D062845:Chloroacetates; D004249:DNA Damage; D003999:Dichloroacetic Acid / Q000633:toxicity*; D004926:Escherichia coli / Q000187:drug effects / Q000235:genetics; D006842:Hydrocarbons, Brominated; D015162:Micronucleus Tests; D009152:Mutagenicity Tests; D009153:Mutagens / Q000633:toxicity*; D013014:SOS Response, Genetics / Q000187:drug effects; D012455:Salamandridae; D012486:Salmonella typhimurium / Q000187:drug effects; D014238:Trichloroacetic Acid / Q000633:toxicity; D014881:Water Supply
D000085:Acetates; D062845:Chloroacetates; D006842:Hydrocarbons, Brominated; D009153:Mutagens; C006972:chloroacetic acid; D014238:Trichloroacetic Acid; C016274:bromoacetate; C017766:tribromoacetic acid; C088674:dibromoacetic acid; D003999:Dichloroacetic Acid