[Detecting immunoreactive GH mRNA in human peripheral blood lymphocytes].
The present study demonstrats that immunoreactive GH mRNA is expressed at low level in normal human peripheral blood lymphocytes by RT-PCR amplification and hybridization. Northern blot analysis of total RNA from lymphocytes with a pituitary hGH cDNA probe revealed a faint transcript migrating almost coincident with pituitary hGH menssenger RNA. Reverse transcription-polymerase chain reaction from total RNA of lymphocytes by using hGH specific primers generated a predicted 702 base pair band with almost the same size as pituitary and also hybridized with pituitary hGH cDNA probe specifically in Southern blot analysis, which indicates a low level expression of GH-like mRNA in human peripheral blood lymphocytes.
Liu
J
J
PUMC Hospital, CAMS, Beijing.
Zheng
D
D
Deng
J
J
Shi
Y
Y
D000236:Adenoma / Q000378:metabolism; D015152:Blotting, Northern; D015139:Blotting, Southern; D013006:Growth Hormone / Q000235:genetics* / Q000378:metabolism; D006801:Humans; D008214:Lymphocytes / Q000378:metabolism*; D010911:Pituitary Neoplasms / Q000378:metabolism; D016133:Polymerase Chain Reaction / Q000379:methods; D012333:RNA, Messenger / Q000096:biosynthesis
D012333:RNA, Messenger; D013006:Growth Hormone