title: Synthesis and two-dimensional electrophoretic analysis of mixed populations of circular and linear RNAs.
abstract: Spontaneous cleavage of the less abundant form of tobacco ringspot virus satellite RNA is readily reversible. Capitalizing on earlier observations by Feldstein and Bruening that small 'mini-monomer' RNAs derived from this molecule and containing little more than covalently attached ribozyme and substrate cleavage products are able to efficiently circularize, we have constructed a series of self-circularizing RNAs of precisely known size. Mixtures of linear and circular RNAs synthesized in vitro and containing 225-1132 nt could be completely resolved using a novel two-dimensional denaturing polyacrylamide gel electrophoresis system. Similar analyses of a complex mixture of coconut cadang-cadang viroid RNAs revealed the presence of relatively large amounts of a previously undescribed 'fast-slow' heterodimeric RNA species in infected palms. Only a single DNA template is required to prepare each pair of circular and linear RNA markers.
authors.affiliation: Plant Biology Department, University of Maryland, College Park, MD 20742, USA.
authors.forename: P A
authors.initials: PA
authors.lastname: Feldstein
authors.forename: L
authors.initials: L
authors.lastname: Levy
authors.forename: J W
authors.initials: JW
authors.lastname: Randles
authors.forename: R A
authors.initials: RA
authors.lastname: Owens
mesh_terms: D001483:Base Sequence; D015180:Electrophoresis, Gel, Two-Dimensional; D005821:Genetic Techniques; D008969:Molecular Sequence Data; D017860:Nepovirus / Q000235:genetics; D009690:Nucleic Acid Conformation; D012313:RNA / Q000138:chemical synthesis* / Q000737:chemistry; D019244:RNA, Satellite / Q000737:chemistry; D012367:RNA, Viral / Q000737:chemistry
chemical_list: D019244:RNA, Satellite; D012367:RNA, Viral; D012313:RNA