Trp64Arg substitution in the beta 3-adrenergic receptor does not relate to body weight in healthy, premenopausal women.
OBJECTIVE
To examine the contribution of the substitution of arginine for tryptophan at amino acid position 64 (Trp64Arg) of the beta 3-adrenergic receptor to body weight and weight-related factors in healthy, premenopausal women.


METHODS
Five hundred and five healthy, premenopausal women 44-50 y, body mass index (BMI) 20-40 kg/m2.


METHODS
Subjects were genotyped for the Trp64Arg substitution using restriction fragment length polymorphism (RFLP) analysis. Measurements for weight and weight-related factors (for example, weight, waist-hip ratio, lipid levels) were also taken.


RESULTS
Two women were homozygous Arg/Arg, 75 women were heterozygous Trp/Arg, and 428 women were homozygous Trp/Trp. The frequency of the Arg substitution was 0.078. When subjects with and without the arginine substitution were compared, no significant differences were found on measures of weight or weight-related risk factors.


CONCLUSIONS
The results confirm previous studies suggesting that the Trp64Arg substitution of the beta 3-adrenergic receptor is not related to weight or weight-related phenotypes in healthy, premenopausal women.
Moriarty
M
M
Department of Human Genetics, University of Pittsburgh, Graduate School of Public Health, PA 15213, USA.
Wing
R R
RR
Kuller
L H
LH
Ferrell
R E
RE
D000483:Alleles; D000886:Anthropometry; D001483:Base Sequence; D001835:Body Weight / Q000235:genetics; D017931:DNA Primers / Q000737:chemistry; D003924:Diabetes Mellitus, Type 2 / Q000208:ethnology / Q000235:genetics; D005260:Female; D005787:Gene Frequency; D005838:Genotype; D006579:Heterozygote; D006720:Homozygote; D006801:Humans; D008875:Middle Aged; D009765:Obesity / Q000208:ethnology / Q000235:genetics*; D017354:Point Mutation / Q000235:genetics*; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length; D017697:Premenopause / Q000235:genetics*; D011943:Receptors, Adrenergic, beta / Q000737:chemistry / Q000235:genetics*; D022702:Receptors, Adrenergic, beta-3; D014364:Tryptophan / Q000235:genetics
D017931:DNA Primers; D011943:Receptors, Adrenergic, beta; D022702:Receptors, Adrenergic, beta-3; D014364:Tryptophan