High-performance thin-layer chromatography for the determination of nimesulide in human plasma, and its use in pharmacokinetic studies.
A rapid and sensitive high-performance thin-layer chromatographic assay has been developed for the measurement of nimesulide in human plasma. Its use for pharmacokinetic studies has been evaluated. The method includes a single-stage extraction procedure without the use of an internal standard. Analysis was performed on plasma containing known amounts of the drug, on drug-free plasma, and on plasma containing an unknown quantity of the drug. Known amounts of extract and nimesulide (100 and 200 ng, as external standard) were spotted on precoated silica-gel 60F254 plates by means of a Camag Linomat IV autosampler. Quantification was achieved using a Camag TLC scanner 3. The recovery of the method was 97.10 +/- 2.22%. The method was applied for the determination of plasma levels and pharmacokinetic parameters of nimesulide after oral administration of two formulations (100 mg) in healthy volunteers. The method is a sensitive, economical, rapid and specific assay for nimesulide in human plasma, and is suitable for pharmacokinetic studies after therapeutic doses.
Pandya
K K
KK
Department of Phytochemistry, Cadila Pharmaceuticals Ltd., Ahmedabad, India.
Satia
M C
MC
Modi
I A
IA
Modi
R I
RI
Chakravarthy
B K
BK
Gandhi
T P
TP
D000284:Administration, Oral; D000328:Adult; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000097:blood* / Q000493:pharmacokinetics; D001682:Biological Availability; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D002855:Chromatography, Thin Layer / Q000379:methods; D018592:Cross-Over Studies; D006801:Humans; D008297:Male; D012680:Sensitivity and Specificity; D013449:Sulfonamides / Q000097:blood* / Q000493:pharmacokinetics; D013607:Tablets
D000894:Anti-Inflammatory Agents, Non-Steroidal; D013449:Sulfonamides; D013607:Tablets; C012655:nimesulide