title: Growth hormone (GH) regulation of submandibular gland structure and function in the GH-deficient rat: upregulation of haptocorrin.
abstract: Based on localization studies of the GH receptor/binding protein (BP) in the gastrointestinal tract, we have recently demonstrated growth hormone regulation of gastric intrinsic factor. In order to define the role of GH in the submandibular gland (SMG) we have investigated the effect of GH on SMG structure and function with particular reference to haptocorrin. Bovine GH (65 micrograms/100 g body weight) was administered twice daily to adult male dwarf rats for 6 days (DW+) while control animals received vehicle (DW-). Administration of GH produced a significant increase in body weight (P < 0.001) and allometric increase in SMG weight (P = 0). There was no change in RNA or protein content per g SMG and GH administration produced a small decrease in DNA content normalized to SMG weight. Morphometric analysis of the SMG revealed a significant increase in the percentage area of the gland occupied by tubular (GH receptor/BP expressing) structures and a significant increase in the diameter of both the intralobular striated and granular convoluted tubules. The effect of GH on cellular proliferation in the ductular and acinar components was determined by the immunohistochemical detection of nuclear 5'-bromo-2'-deoxyuridine (BrdU) incorporated during a 2-h pulse of BrdU. GH treatment induced a 5.5-fold increase in the labelling index of tubular cells whereas the acinar cell labelling index increased only 3.3-fold. Soluble extracts of SMG were prepared for estimation of 57Co-cyanocobalamin (vitamin B12) binding. GH administration resulted in an increase in total 57Co-cyanocobalamin (CBL) binding per mg SMG protein. To determine the contribution of haptocorrin (R-protein) the amount of cobinamide dicyanide (CD) displaceable binding was calculated. GH administration produced a 70% increase in CD displaceable CBL binding per mg SMG indicating GH regulation of haptocorrin. A comparison of total SMG CBL binding and CD displaceable CBL binding between male and female rats detected no sex difference. Therefore sex-specific GH secretory profiles are unlikely to be of importance in the regulation of haptocorrin. In conclusion we have demonstrated that GH stimulates hypertrophy and hyperplasia of components of the SMG in the dwarf rat. The observed upregulation of haptocorrin may synergize with the GH-stimulated increase in intrinsic factor to facilitate absorption of CBL during the anabolic state.
authors.affiliation: Institute of Molecular and Cell Biology, National University of Singapore, Singapore.
authors.forename: P E
authors.initials: PE
authors.lastname: Lobie
authors.forename: M J
authors.initials: MJ
authors.lastname: Waters
mesh_terms: D000818:Animals; D001835:Body Weight / Q000187:drug effects; D004247:DNA / Q000032:analysis; D006130:Growth Disorders / Q000188:drug therapy / Q000378:metabolism / Q000473:pathology*; D013006:Growth Hormone / Q000172:deficiency / Q000502:physiology* / Q000627:therapeutic use; D008297:Male; D009687:Nuclear Proteins / Q000378:metabolism; D009929:Organ Size / Q000187:drug effects; D011485:Protein Binding; D011506:Proteins / Q000032:analysis; D012313:RNA / Q000032:analysis; D051381:Rats; D011917:Rats, Inbred Lew; D013363:Submandibular Gland / Q000187:drug effects / Q000378:metabolism / Q000473:pathology*; D014805:Vitamin B 12 / Q000378:metabolism
chemical_list: D009687:Nuclear Proteins; D011506:Proteins; D012313:RNA; D013006:Growth Hormone; D004247:DNA; D014805:Vitamin B 12