title: Cortisol inhibits glycosaminoglycan synthesis in cultured rainbow trout cartilage.
abstract: In vitro actions of corticosteroids (cortisol, 11-deoxycortisol, cortisone, and corticosterone) as well as interaction between cortisol and triiodothyronine (T3) or recombinant human insulin-like growth factor-I (rhIGF-I) on cartilage glycosaminoglycan (GAG) synthesis in rainbow trout (Oncorhynchus mykiss) were examined. Uptake of [35S]sulfate by isolated branchial cartilage was measured as a marker for GAG synthesis. In vitro exposure of cartilage to cortisol at concentrations of 10, 100, and 1000 nM for 6 days dose-dependently inhibited sulfate uptake, while exposure to 0.1 and 1 nM cortisol had no effect. Corticosterone and 11-deoxycortisol at concentrations of 10 and 100 nM inhibited sulfate uptake slightly but not dose-dependently. Cortisone (1, 10, and 100 nM) had no effect. When cortisol (1, 10, and 100 nM) and T3 (0.075 and 0.75 nM) were simultaneously added to the culture, the T3-induced sulfate uptake was dose-dependently reduced by the presence of 10 and 100 nM cortisol. When cortisol (1, 10, and 100 nM) and rhIGF-I (0.1 and 1 nM) were added together, the sulfate uptake induced by 0.1 nM rhIGF-I was only slightly inhibited by 100 nM cortisol, but 1 nM rhIGF-I completely masked the inhibitory effect of cortisol. These data suggest that GAG synthesis in the rainbow trout cartilage is controlled by multiple interactions among stimulative hormones, such as T3 and IGF-I, and inhibitory hormones, such as cortisol.
authors.affiliation: Ocean Research Institute, University of Tokyo, Akahama, Iwate, 028-11, Japan.
authors.forename: Y
authors.initials: Y
authors.lastname: Takagi
authors.forename: B T
authors.initials: BT
authors.lastname: Björnsson
mesh_terms: D000305:Adrenal Cortex Hormones / Q000494:pharmacology; D000818:Animals; D002356:Cartilage / Q000187:drug effects / Q000378:metabolism*; D003470:Culture Media; D004305:Dose-Response Relationship, Drug; D006025:Glycosaminoglycans / Q000096:biosynthesis*; D006801:Humans; D006854:Hydrocortisone / Q000494:pharmacology; D007334:Insulin-Like Growth Factor I / Q000494:pharmacology; D017686:Oncorhynchus mykiss / Q000378:metabolism*; D009924:Organ Culture Techniques; D011994:Recombinant Proteins / Q000494:pharmacology; D013462:Sulfur Radioisotopes; D014284:Triiodothyronine / Q000494:pharmacology
chemical_list: D000305:Adrenal Cortex Hormones; D003470:Culture Media; D006025:Glycosaminoglycans; D011994:Recombinant Proteins; D013462:Sulfur Radioisotopes; D014284:Triiodothyronine; D007334:Insulin-Like Growth Factor I; D006854:Hydrocortisone