title: Massive glycation of protein HC, a low molecular weight lipocalin, in non-diabetic individuals.
abstract: Human protein HC is a member of the lipocalin superfamily with unique properties since it carries a covalently bound fluorescent chromophore mediating the linkage of the major part of protein HC to several plasma proteins, with IgA as the dominating complex partner. Native protein HC displays characteristic absorption and fluorescence spectra similar to those of glycated proteins with advanced glycosylation end products (AGEs). In vitro glycation of protein HC induces the formation of fibril aggregates with a corresponding increase of absorption in the visible region of the spectrum. Boronate-affinity chromatography and a novel galactosyltransferase assay indicate that protein HC is modified with residues of glucose exposed in a terminal non-reducing position which is typical of glycated proteins. The glycation level of several isolated batches of protein HC as measured by both assays was around 35%, which represents the highest level described for human plasma-derived proteins from healthy individuals.
authors.affiliation: Unidad de Análisis Estructural de Proteínas, Centro Nacional de Biotecnología, Madrid, Spain.
authors.forename: P
authors.initials: P
authors.lastname: Bonay
authors.forename: J
authors.initials: J
authors.lastname: Solís
authors.forename: H
authors.initials: H
authors.lastname: de la Calle
authors.forename: M
authors.initials: M
authors.lastname: Fresno
authors.forename: A
authors.initials: A
authors.lastname: Grubb
authors.forename: E
authors.initials: E
authors.lastname: Méndez
mesh_terms: D000510:Alpha-Globulins / Q000737:chemistry* / Q000378:metabolism / Q000652:urine; D002846:Chromatography, Affinity; D002851:Chromatography, High Pressure Liquid; D003124:Colorimetry; D003920:Diabetes Mellitus; D005700:Galactosyltransferases; D006026:Glycoside Hydrolases; D006031:Glycosylation; D006801:Humans; D011480:Protease Inhibitors / Q000737:chemistry* / Q000378:metabolism / Q000652:urine; D012016:Reference Values
chemical_list: D000510:Alpha-Globulins; D011480:Protease Inhibitors; C016001:alpha-1-microglobulin; D005700:Galactosyltransferases; D006026:Glycoside Hydrolases