title: Ras-GRF, the activator of Ras, is expressed preferentially in mature neurons of the central nervous system.
abstract: In rodents, the Ras-specific guanine-nucleotide exchange factor (Ras-GRF) is expressed in different areas of the brain and, at a reduced level, also in the spinal cord. No expression of the 140 kDa Ras-GRF was detected in dorsal root ganglia and all other tissues tested. Analysis of primary cultures derived from brain reveals that this exchange factor is only present in neurons of the central nervous system. In primary hippocampal cultures, the expression of Ras-GRF increases in parallel with the onset of a neuronal network and in the whole brain it increases sharply after birth.
authors.affiliation: Department of General Physiology and Biochemistry, University of Milan, Italy.
authors.forename: R
authors.initials: R
authors.lastname: Zippel
authors.forename: N
authors.initials: N
authors.lastname: Gnesutta
authors.forename: N
authors.initials: N
authors.lastname: Matus-Leibovitch
authors.forename: E
authors.initials: E
authors.lastname: Mancinelli
authors.forename: D
authors.initials: D
authors.lastname: Saya
authors.forename: Z
authors.initials: Z
authors.lastname: Vogel
authors.forename: E
authors.initials: E
authors.lastname: Sturani
authors.forename: Z
authors.initials: Z
authors.lastname: Renata
authors.forename: G
authors.initials: G
authors.lastname: Nerina
authors.forename: M L
authors.initials: ML
authors.lastname: Noa
authors.forename: M
authors.initials: M
authors.lastname: Enzo
authors.forename: S
authors.initials: S
authors.lastname: Daniella
authors.forename: V
authors.initials: V
authors.lastname: Zvi
authors.forename: S
authors.initials: S
authors.lastname: Emmapaola
mesh_terms: D000818:Animals; D001253:Astrocytes / Q000166:cytology / Q000378:metabolism; D001921:Brain / Q000166:cytology / Q000378:metabolism*; D002147:Calmodulin / Q000032:analysis; D002478:Cells, Cultured; D004622:Embryo, Mammalian; D005727:Ganglia, Spinal / Q000166:cytology / Q000378:metabolism; D020662:Guanine Nucleotide Exchange Factors; D009457:Neuroglia / Q000166:cytology / Q000378:metabolism; D009474:Neurons / Q000166:cytology / Q000378:metabolism*; D009928:Organ Specificity; D014176:Protein Biosynthesis; D011506:Proteins / Q000032:analysis; D051381:Rats; D017208:Rats, Wistar; D013116:Spinal Cord / Q000166:cytology / Q000378:metabolism*; D020663:ras Guanine Nucleotide Exchange Factors; D020666:ras-GRF1
chemical_list: D002147:Calmodulin; D020662:Guanine Nucleotide Exchange Factors; D011506:Proteins; D020663:ras Guanine Nucleotide Exchange Factors; D020666:ras-GRF1