title: Spectroscopic study of secondary structure and thermal denaturation of recombinant human factor XIII in aqueous solution.
abstract: The secondary structure and thermal denaturation (in H2O vs D2O) of recombinant human factor XIII in aqueous solutions were investigated using infrared and circular dichroism (CD) spectroscopies. The infrared amide I spectrum of the protein in H2O solution at 25 degrees C exhibited an absorbance maximum near 1642 cm-1, indicating the presence of a predominantly beta-sheet structure. Quantitative analysis revealed that the native protein contains 13-16% alpha-helix, 41-49% beta-sheet, 29% beta-turn, and 10-14% extended strand structures. The presence of a strong low-wavenumber beta-sheet band at 1641 cm-1 and a weak high-wavenumber beta-sheet band at 1689 cm-1 indicated that the beta-sheet structure of the protein is predominantly antiparallel. Quantitative analysis of the CD spectrum using the SELCON method indicated a secondary structural content of 10% alpha-helix, 40-50% beta-sheet, 20-35% beta-turns, and 20-35% unordered elements, which matches that determined by X-ray crystallography. The apparent discrepancy with the contents of unordered element determined by infrared spectroscopy is reconciled by considering that CD spectroscopy and X-ray crystallography assign extended loops and strands to unordered elements, whereas infrared spectroscopy recognizes these as distinct structured elements. During heating above 60 degrees C, a pair of new infrared bands appeared at 1626 and 1693 cm-1 for the protein in H2O and 1619 and 1683 cm-1 in D2O, indicating a formation of intermolecular beta-sheet aggregates. The intensities of the new bands increased as a function of temperature, concomitant with an intensity decrease in bands for the native protein structural elements. As expected, there was an increase in thermal stability in D2O relative to that in H2O, which was manifested as an increase of about 5 degrees C in the temperature for initial loss of infrared bands assigned to native structural elements and for appearance of bands due to intermolecular beta-sheet. In addition, the midpoint of the thermally induced transitions in infrared spectra were about 2.5 degrees C higher in D2O than in H2O. Based on the infrared analysis, the thermally denatured state of the protein in both H2O and D2O contains predominantly intermolecular beta-sheet. The broad, poorly resolved absorbance that spans the region between the intermolecular beta-sheet bands was assigned to an ensemble of heterogeneous structural elements (including unordered), none of which is populated to a high enough degree to result in a distinct infrared band. Results from CD spectroscopy support these conclusions about the structure of the denatured, aggregated protein.
authors.affiliation: Department of Chemistry and Biochemistry, University of Northern Colorado, Greeley, Colorado 80639, USA.
authors.forename: A
authors.initials: A
authors.lastname: Dong
authors.forename: B
authors.initials: B
authors.lastname: Kendrick
authors.forename: L
authors.initials: L
authors.lastname: Kreilgârd
authors.forename: J
authors.initials: J
authors.lastname: Matsuura
authors.forename: M C
authors.initials: MC
authors.lastname: Manning
authors.forename: J F
authors.initials: JF
authors.lastname: Carpenter
mesh_terms: D002942:Circular Dichroism; D017666:Deuterium Oxide; D005169:Factor VIII / Q000737:chemistry*; D006358:Hot Temperature; D006801:Humans; D011489:Protein Denaturation; D017433:Protein Structure, Secondary; D011994:Recombinant Proteins / Q000737:chemistry; D012996:Solutions; D013055:Spectrophotometry, Infrared; D014867:Water
chemical_list: D011994:Recombinant Proteins; D012996:Solutions; D014867:Water; D005169:Factor VIII; D017666:Deuterium Oxide