title: [Mutations of p53 gene exons 5 to 8 in human ovarian cancer and its clinical significance].
abstract: OBJECTIVE
  To detect p53 mutations in human ovarian cancer and to look for correlations between p53 mutation and clinicopathologic features.
  
  
  METHODS
  Mutations in exons 5-8 of p53 gene in 41 patients with primary ovarian cancer were screened by polymerase chain reaction-single strand conformation polymorphism analysis with non-isotopic silver staining method.
  
  
  RESULTS
  Mutations of p53 were found in 17 cases (41.5%); p53 mutation was not correlated with the age, the FIGO stage and the histologic type of the patients. However, it was associated with grade and lymph node metastasis in a univariate analysis.
  
  
  CONCLUSIONS
  Mutations of p53 occur commonly in human ovarian cancer. p53 mutation is an early event in ovarian carcinogenesis and may serve as a genetic marker for early diagnosis and prognosis.
authors.affiliation: First Affiliated Hospital, Beijing Medical University.
authors.forename: Z
authors.initials: Z
authors.lastname: You
authors.forename: Y
authors.initials: Y
authors.lastname: Guo
authors.forename: H
authors.initials: H
authors.lastname: Wen
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D005091:Exons; D005260:Female; D016158:Genes, p53 / Q000235:genetics*; D006801:Humans; D008207:Lymphatic Metastasis; D008875:Middle Aged; D009154:Mutation; D010051:Ovarian Neoplasms / Q000235:genetics* / Q000473:pathology; D016133:Polymerase Chain Reaction; D018807:Polymorphism, Single-Stranded Conformational