Conventional capillary electrophoresis in comparison with short-capillary capillary electrophoresis and microfabricated glass chip capillary electrophoresis for the analysis of fluorescein isothiocyanate anti-human immunoglobulin G.
Fast, efficient analysis of fluorescein isothiocyanate anti-human IgG was achieved in a short-capillary and in a microfabricated glass chip. The capillary was 6 cm long from injection end to detector with electric field strength of 0.268 kV/cm. Analyses were performed within 1 min. A glass microchip device was fabricated using standard photolithographic procedures and chemical wet etching. The channels were sealed using a direct bonding technique. For an effective length of 2.8 cm with electric field strength of 0.526 kV/cm, electrophoretic analysis was achieved in less than 16 s. Conventional capillary electrophoresis gave highly reproducible results and good detection limits. Analysis time was 2.5 min for a capillary with 47 cm effective length and electric field strength of 0.383 kV/cm.
Rodriguez
I
I
Department of Chemistry, National University of Singapore, Singapore.
Zhang
Y
Y
Lee
H K
HK
Li
S F
SF
D019075:Electrophoresis, Capillary / Q000379:methods*; D016650:Fluorescein-5-isothiocyanate / Q000737:chemistry*; D005456:Fluorescent Dyes / Q000737:chemistry*; D006801:Humans; D007074:Immunoglobulin G / Q000737:chemistry / Q000276:immunology*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013050:Spectrometry, Fluorescence
D005456:Fluorescent Dyes; D007074:Immunoglobulin G; D016650:Fluorescein-5-isothiocyanate