[Use of photoactivation in the preparation of radiopharmaceuticals. II. Dialysate of human leukocytes labelled with technetium (99mTc)].
The paper evaluates the effect of photoactivation on dialysate of human leukocytes, on their labelling with technetium(99mTc), and on biodistribution in rats. Dialysate of human leukocytes was irradiated with UV light at 254 nm and labelled with technetium(99mTc) by reduction of pertechnetate(99mTc) with stannous chloride. Radiochemical purity was evaluated using paper chromatography. The biodistribution of labelled dialysate of human leukocytes in rats with experimentally induced inflammation was determined by counting the activity of isolated organs or by planar gamma camera imaging 4 hours after administration. Photoactivation mildly raises the number of free SH groups in leukocyte dialysate. A high labelling efficacy (97%) and stability 8 hours after labelling (94%) was achieved. An effect of radiation on labelling efficacy and stability was not demonstrated. Photoactivation has a beneficial effect on the distribution of 99mTc dialysate of human leukocytes into experimental inflammation as measured in isolated organs of rats; however, deposition into inflammatory lesions is not quite sufficient for scintigraphic imaging.
Kleisner
I
I
Klinika nukleární medicíny FNKV, Praha.
Komárek
P
P
Konopková
M
M
Komárková
I
I
D000818:Animals; D006801:Humans; D007962:Leukocytes / Q000528:radiation effects; D019275:Radiopharmaceuticals; D051381:Rats; D017208:Rats, Wistar; D013667:Technetium; D014466:Ultraviolet Rays
D019275:Radiopharmaceuticals; D013667:Technetium