title: Clinical field site evaluation of the FACSCount for absolute CD3+, CD3+ CD4+, and CD3+ CD8+ cell count determinations in Thailand.
abstract: The FACSCount flow cytometer absolute CD3+, CD3+ CD4+, and CD3+ CD8+ cell counts measured at a field site hospital laboratory in Thailand were compared to FACScan absolute counts obtained at a nearby research laboratory. Correlation coefficients for 208 samples were > or = 0.95. The FACSCount was accurate, and it was easier and less expensive to operate than the FACScan. Additionally, the FACScan-generated lymphocyte percentage value was accurate for use with the FACScan SimulSET software.
authors.affiliation: HIV/AIDS Collaboration and Department of Communicable Disease Control, Central Chest Hospital, Nonthaburi, Thailand. NLY0@cdc.gov
authors.forename: N L
authors.initials: NL
authors.lastname: Young
authors.forename: P
authors.initials: P
authors.lastname: Ponglertnapakorn
authors.forename: N
authors.initials: N
authors.lastname: Shaffer
authors.forename: K
authors.initials: K
authors.lastname: Srisak
authors.forename: T
authors.initials: T
authors.lastname: Chaowanachan
authors.forename: V
authors.initials: V
authors.lastname: On-Thern
authors.forename: C
authors.initials: C
authors.lastname: Kittinunvorakoon
authors.forename: A
authors.initials: A
authors.lastname: Bunwattanakul
authors.forename: S
authors.initials: S
authors.lastname: Suksaweang
authors.forename: V
authors.initials: V
authors.lastname: Pobkeeree
authors.forename: J
authors.initials: J
authors.lastname: Punnotok
authors.forename: T D
authors.initials: TD
authors.lastname: Mastro
mesh_terms: D017252:CD3 Complex / Q000032:analysis*; D015704:CD4 Antigens / Q000032:analysis*; D016827:CD8 Antigens / Q000032:analysis*; D005434:Flow Cytometry / Q000379:methods*; D015658:HIV Infections / Q000097:blood; D006679:HIV Seropositivity; D006801:Humans; D007958:Leukocyte Count / Q000379:methods*; D014376:Tuberculosis / Q000097:blood
chemical_list: D017252:CD3 Complex; D015704:CD4 Antigens; D016827:CD8 Antigens