Neuronal recovery after moderate hypoxia is improved by the calpain inhibitor MDL28170.
The role of calcium-activated proteolysis in hypoxic neuronal injury was investigated using an in vitro slice model of moderate hypoxia that mimics many features of an ischemic penumbra. The calpain inhibitor, MDL28170, significantly improved the recovery of synaptic responses in hippocampal slices following prolonged, moderate hypoxia without hypoxic depolarization. This finding further implicates calpain-mediated proteolysis in the development of neuronal injury following moderate metabolic challenge such as occurs in regions of partial ischemia.
Chen
Z F
ZF
Department of Neurological Surgery, Health Sciences Center, University of Virginia, Charlottesville 22908, USA.
Schottler
F
F
Lee
K S
KS
D000818:Animals; D002154:Calpain / Q000037:antagonists & inhibitors* / Q000502:physiology; D015853:Cysteine Proteinase Inhibitors / Q000494:pharmacology*; D004151:Dipeptides / Q000494:pharmacology*; D019706:Excitatory Postsynaptic Potentials / Q000187:drug effects; D006624:Hippocampus / Q000187:drug effects / Q000503:physiopathology; D000860:Hypoxia / Q000503:physiopathology*; D008297:Male; D009474:Neurons / Q000187:drug effects* / Q000502:physiology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D013569:Synapses / Q000187:drug effects / Q000502:physiology
D015853:Cysteine Proteinase Inhibitors; D004151:Dipeptides; D002154:Calpain; C058076:calpain inhibitor III