An evaluation of selected DNA extraction strategies for short tandem repeat typing.
Organic and Chelex extraction methods are compared to five commercially available DNA extraction kits. For forensic purposes, comparison of different extraction strategies is based on the ability of the methods to economically recover high yields of amplifiable DNA from small blood samples in a quick, simple and safe manner. The findings of this study indicate that resin-based methods, such as Chelex, are best able to meet these criteria.
Vandenberg
N
N
Victoria Forensic Science Centre, Macleod, Australia.
van Oorschot
R A
RA
Mitchell
R J
RJ
D004247:DNA / Q000097:blood*; D016172:DNA Fingerprinting / Q000379:methods*; D005069:Evaluation Studies as Topic; D005554:Forensic Medicine; D006801:Humans; D007475:Ion Exchange Resins; D018598:Minisatellite Repeats; D011137:Polystyrenes; D011145:Polyvinyls; D011933:Reagent Kits, Diagnostic
D007475:Ion Exchange Resins; D011137:Polystyrenes; D011145:Polyvinyls; D011933:Reagent Kits, Diagnostic; C006960:chelex; D004247:DNA