Interphase cytogenetics in pathology: principles, methods, and applications of fluorescence in situ hybridization (FISH).
Characteristic chromosome aberrations have been identified in various tumors. Fluorescence in situ hybridization (FISH) using specific probes that are generated by vector cloning or in vitro amplification and labeled with fluorescent dyes allow for the detection of these genetic changes in interphase cells. This technique, that is also referred to as "interphase cytogenetics", can be performed in cytological preparations as well as in sections of routinely formaldehyde-fixed and paraffin-embedded tissue. In cancer research and diagnostics, interphase cytogenetics by FISH is used to detect numerical chromosome changes and structural aberrations, e.g., translocations, deletions, or amplifications. In this technical overview, we explain the principles of the FISH method and provide protocols for FISH in cytological preparations and paraffin sections. Moreover, possible applications of FISH are discussed.
Werner
M
M
Institute of Pathology, Technische Universität München, Munich, Germany.
Wilkens
L
L
Aubele
M
M
Nolte
M
M
Zitzelsberger
H
H
Komminoth
P
P
D003584:Cytological Techniques; D015342:DNA Probes; D006801:Humans; D017404:In Situ Hybridization, Fluorescence / Q000379:methods*; D007399:Interphase; D012091:Repetitive Sequences, Nucleic Acid
D015342:DNA Probes