Activation of ADP-ribosylation factor 1 GTPase-activating protein by phosphatidylcholine-derived diacylglycerols.
Disassembly of the coatomer from Golgi vesicles requires that the small GTP-binding protein ADP-ribosylation factor 1 (ARF1) hydrolyzes its bound GTP by the action of a GTPase-activating protein. In vitro, the binding of the ARF1 GTPase-activating protein to lipid vesicles and its activity on membrane-bound ARF1GTP are increased by diacylglycerols with monounsaturated acyl chains, such as those arising in vivo as secondary products from the hydrolysis of phosphatidylcholine by ARF-activated phospholipase D. Thus, the phospholipase D pathway may provide a feedback mechanism that promotes GTP hydrolysis on ARF1 and the consequent uncoating of vesicles.
Antonny
B
B
CNRS, Institut de Pharmacologie Moléculaire et Cellulaire, 660 route des lucioles, 06560 Valbonne, France. antonny@unice.fr
Huber
I
I
Paris
S
S
Chabre
M
M
Cassel
D
D
D020823:ADP-Ribosylation Factor 1; D020727:ADP-Ribosylation Factors; D002352:Carrier Proteins / Q000378:metabolism*; D004075:Diglycerides / Q000378:metabolism*; D004305:Dose-Response Relationship, Drug; D004789:Enzyme Activation; D004926:Escherichia coli; D020558:GTP Phosphohydrolases / Q000378:metabolism*; D019204:GTP-Binding Proteins / Q000378:metabolism*; D020690:GTPase-Activating Proteins; D006153:Guanosine Diphosphate / Q000378:metabolism; D006160:Guanosine Triphosphate / Q000378:metabolism; D006868:Hydrolysis; D008956:Models, Chemical; D010713:Phosphatidylcholines; D010739:Phospholipase D / Q000378:metabolism; D011506:Proteins / Q000378:metabolism*
D002352:Carrier Proteins; D004075:Diglycerides; D020690:GTPase-Activating Proteins; D010713:Phosphatidylcholines; D011506:Proteins; D006153:Guanosine Diphosphate; D006160:Guanosine Triphosphate; D010739:Phospholipase D; D020558:GTP Phosphohydrolases; D019204:GTP-Binding Proteins; D020823:ADP-Ribosylation Factor 1; D020727:ADP-Ribosylation Factors; C013965:diolein