title: Determination of saquinavir in human plasma, saliva, and cerebrospinal fluid by ion-pair high-performance liquid chromatography with ultraviolet detection.
abstract: A high-performance liquid chromatographic method for the determination of the HIV protease inhibitor saquinavir in human plasma, saliva, and cerebrospinal fluid is described. Saquinavir was extracted from samples using C2 extraction columns prior to ion-pair, reversed-phase high-performance liquid chromatography with ultraviolet detection at 239 nm. The method has been validated over the range of 2.5-4000 ng/ml using a 0.6-ml sample volume. This assay has been used for the analysis of saquinavir in plasma and saliva of HIV-1-infected patients.
authors.affiliation: Department of Pharmacy, Slotervaart Hospital, Amsterdam, The Netherlands.
authors.forename: R M
authors.initials: RM
authors.lastname: Hoetelmans
authors.forename: M
authors.initials: M
authors.lastname: van Essenberg
authors.forename: P L
authors.initials: PL
authors.lastname: Meenhorst
authors.forename: J W
authors.initials: JW
authors.lastname: Mulder
authors.forename: J H
authors.initials: JH
authors.lastname: Beijnen
mesh_terms: D019380:Anti-HIV Agents / Q000097:blood / Q000134:cerebrospinal fluid / Q000493:pharmacokinetics*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D015658:HIV Infections / Q000378:metabolism; D017320:HIV Protease Inhibitors / Q000097:blood / Q000134:cerebrospinal fluid / Q000493:pharmacokinetics*; D006801:Humans; D015203:Reproducibility of Results; D012463:Saliva / Q000378:metabolism*; D019258:Saquinavir / Q000032:analysis* / Q000097:blood / Q000134:cerebrospinal fluid / Q000493:pharmacokinetics; D012680:Sensitivity and Specificity; D013056:Spectrophotometry, Ultraviolet
chemical_list: D019380:Anti-HIV Agents; D017320:HIV Protease Inhibitors; D019258:Saquinavir