title: Oxygen sensitivity of mitochondrial metabolic state in isolated skeletal and cardiac myocytes.
abstract: In striated muscle the coupling of blood flow to changes in tissue metabolism is hypothesized to be dependent in part on release of vasodilating metabolic by-products generated when mitochondrial metabolism becomes O2 limited. Cytochrome oxidase, the terminal step in oxidative phosphorylation, is half-maximally saturated at < 1 mmHg PO2 in isolated mitochondria. However, blood flow is regulated at tissue PO2 of approximately 20 mmHg. If the affinity of mitochondrial respiration for O2 were higher in vivo than in vitro, O2 limitation of mitochondrial metabolism near mean tissue levels could occur. In the present study the PO2 at which mitochondrial metabolism becomes inhibited (critical PO2) was measured for cardiac myocytes in suspension (1.1 +/- 0.15 mmHg) and single cells (1.0 +/- 0.22 and 1.25 +/- 0.22 mmHg in cardiac myocytes and rat spinotrapezius cells, respectively). These measurements are consistent with those from isolated mitochondria, indicating that vasodilators produced when oxidative phosphorylation becomes inhibited may be important for regulating blood flow only in highly glycolytic muscles or under conditions of severe O2 limitation.
authors.affiliation: Department of Physiology, University of Arizona, Tucson 85724, USA.
authors.forename: K N
authors.initials: KN
authors.lastname: Richmond
authors.forename: S
authors.initials: S
authors.lastname: Burnite
authors.forename: R M
authors.initials: RM
authors.lastname: Lynch
mesh_terms: D000818:Animals; D015687:Cell Hypoxia; D002470:Cell Survival; D002478:Cells, Cultured; D008297:Male; D008929:Mitochondria, Heart / Q000378:metabolism*; D008931:Mitochondria, Muscle / Q000378:metabolism*; D018482:Muscle, Skeletal / Q000166:cytology*; D009206:Myocardium / Q000166:cytology*; D009243:NAD / Q000378:metabolism; D010100:Oxygen / Q000494:pharmacology*; D010101:Oxygen Consumption; D010313:Partial Pressure; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D009243:NAD; D010100:Oxygen