title: Spontaneous polyploidization results in apoptosis in a Meth-A tumor cell line.
abstract: Cultured Meth-A cells always include a small fraction of large cells, which had a DNA content above 4c (polyploid cells). The process from the formation to the disintegration of polyploid Meth-A cells was measured by means of time-lapse videography. Polyploid Meth-A cells arose spontaneously from normal cells (polyploidization), then died by apoptosis. The fraction of polyploid cells gradually increased in seven day-exponential cultures with a low concentration of demecolcine, which is a specific inhibitor of cell division. The results revealed that the polyploid Meth-A cells are generated from normal cells by failing cell division and that they die by apoptosis.
authors.affiliation: Division of Basic Science, Kanazawa Medical University, Ishikawa, Japan.
authors.forename: K
authors.initials: K
authors.lastname: Fujikawa-Yamamoto
authors.forename: Z P
authors.initials: ZP
authors.lastname: Zong
authors.forename: M
authors.initials: M
authors.lastname: Murakami
authors.forename: S
authors.initials: S
authors.lastname: Odashima
authors.forename: T
authors.initials: T
authors.lastname: Ikeda
authors.forename: Y
authors.initials: Y
authors.lastname: Yoshitake
mesh_terms: D017209:Apoptosis; D002452:Cell Count; D002455:Cell Division / Q000187:drug effects; D053938:DNA Fragmentation; D003703:Demecolcine / Q000494:pharmacology; D006131:Growth Inhibitors / Q000494:pharmacology; D011123:Polyploidy; D014407:Tumor Cells, Cultured
chemical_list: D006131:Growth Inhibitors; D003703:Demecolcine