Creatine phosphate, not ATP, is required for 3' end cleavage of mammalian pre-mRNA in vitro.
The poly(A) tail of a mammalian mRNA is generated by endonucleolytic cleavage and poly(A) addition. Previous studies conducted with nuclear extracts suggested an ATP requirement for the cleavage step. We have reexamined the cofactor requirement, initially with the SV40 late pre-mRNA, which requires for cleavage four protein factors, cleavage and polyadenylation specificity factor, cleavage stimulation factor, cleavage factor I, and cleavage factor II. Using highly purified preparations of these factors, which lacked detectable creatine phosphokinase and ATPase activities, creatine phosphate (CP) was, surprisingly, found to be sufficient to promote efficient cleavage. Although other phosphate compounds substituted poorly or not at all for CP, another phosphoguanidine, arginine phosphate, was fully functional. Notably, ATP was neither necessary nor sufficient, and could in fact inhibit the reaction. Treatment of the purified factors with hexokinase plus glucose (to deplete any contaminating ATP) was without effect, as was addition of EDTA. Using 32P-labeled CP, we found that neither hydrolysis of CP nor phosphate transfer from CP occurred during the cleavage reaction. CP also allowed cleavage of the adenovirus 2 L3 pre-mRNA. However, in this case, ATP both enhanced the reaction and influenced the precise site of cleavage, perhaps reflecting the requirement of poly(A) polymerase for cleavage of this RNA. These results indicate that ATP is not essential for 3' pre-mRNA cleavage and that CP or a related compound can function as a necessary cofactor.
Hirose
Y
Y
Department of Biological Sciences, Columbia University, New York, New York 10027, USA.
Manley
J L
JL
D000255:Adenosine Triphosphate / Q000378:metabolism*; D000256:Adenoviridae / Q000235:genetics; D004492:Edetic Acid / Q000378:metabolism; D006367:HeLa Cells; D006801:Humans; D006868:Hydrolysis; D010725:Phosphocreatine / Q000378:metabolism*; D011062:Polynucleotide Adenylyltransferase / Q000378:metabolism; D012322:RNA Precursors / Q000378:metabolism*; D012367:RNA, Viral / Q000378:metabolism
D012322:RNA Precursors; D012367:RNA, Viral; D010725:Phosphocreatine; D000255:Adenosine Triphosphate; D004492:Edetic Acid; D011062:Polynucleotide Adenylyltransferase