title: Application of capillary electrophoresis-fluorescence line-narrowing spectroscopy for on-line spectral characterization of closely related analytes.
abstract: Capillary electrophoresis (CE) interfaced with low-temperature (4.2 K) fluorescence line-narrowing spectroscopy (FLNS) is used for the separation and spectral characterization of closely related analytes. In this paper, the CE-FLNS system is applied to the analysis of a mixture of deuterated and protonated benzo[a]pyrene, a mixture of structurally similar benzo[a]pyrene and benzo[e]pyrene, and mixtures of dibenzo[a,l]pyrene-derived adenine DNA adducts. The CE-FLNS system provides on-line separation and high-resolution spectroscopic identification of CE-separated analytes, via fingerprint structure of vibrationally resolved FLN spectra at 4.2 K. The combination of the separation power of CE and the spectral selectivity of FLNS provide a methodology that has potential to become a powerful tool for molecular analyte characterization. The main applications of the CE-FLNS system, due to its selectivity, should be in the chemical analysis of structurally similar analytes and applications where analyte purity and detailed structural characterization are required.
authors.affiliation: Ames Laboratory-USDOE, Iowa State University 50011, USA.
authors.forename: D
authors.initials: D
authors.lastname: Zamzow
authors.forename: C H
authors.initials: CH
authors.lastname: Lin
authors.forename: G J
authors.initials: GJ
authors.lastname: Small
authors.forename: R
authors.initials: R
authors.lastname: Jankowiak
mesh_terms: D001580:Benzopyrenes / Q000032:analysis* / Q000737:chemistry; D018736:DNA Adducts / Q000032:analysis*; D019075:Electrophoresis, Capillary / Q000379:methods*; D009862:Online Systems; D013050:Spectrometry, Fluorescence
chemical_list: D001580:Benzopyrenes; D018736:DNA Adducts