title: Organization and expression of the chum salmon insulin-like growth factor II gene.
abstract: IGF-II plays an important role in growth and development of vertebrates. In the present study, the characterization of the first fish IGF-II gene, chum salmon IGF-II, is described. The sIGF-II gene consists of four exons, spanning a region of 9 kbp, that encode the 214 aa IGF-II precursor. While the amino acid sequences of fully processed IGF-II of salmon and mammalian species are very similar, the prepro-peptide sequence deviates extensively in the signal- and E-peptide domains. The transcription initiation site of the sIGF-II gene was localized within a 30 nt region employing RT-PCR. Using sIGF-II promoter-luciferase constructs it was demonstrated that the sIGF-II gene has a relatively strong promoter that contains tissue-specific regulatory elements.
authors.affiliation: Department of Biosynthesis of Nucleic Acids, Institute of Molecular Biology and Genetics, National Academy of Sciences of the Ukraine, Kiev.
authors.forename: A Y
authors.initials: AY
authors.lastname: Palamarchuk
authors.forename: P E
authors.initials: PE
authors.lastname: Holthuizen
authors.forename: W E
authors.initials: WE
authors.lastname: Müller
authors.forename: J S
authors.initials: JS
authors.lastname: Sussenbach
authors.forename: V M
authors.initials: VM
authors.lastname: Kavsan
mesh_terms: D000818:Animals; D001483:Base Sequence; D017931:DNA Primers; D005091:Exons; D005786:Gene Expression Regulation; D006801:Humans; D007335:Insulin-Like Growth Factor II / Q000096:biosynthesis* / Q000235:genetics*; D008969:Molecular Sequence Data; D017685:Oncorhynchus keta / Q000235:genetics*; D017686:Oncorhynchus mykiss; D016133:Polymerase Chain Reaction; D011401:Promoter Regions, Genetic; D011498:Protein Precursors / Q000096:biosynthesis / Q000235:genetics; D011994:Recombinant Proteins / Q000096:biosynthesis; D014162:Transfection; D014407:Tumor Cells, Cultured
chemical_list: D017931:DNA Primers; D011498:Protein Precursors; D011994:Recombinant Proteins; C066038:proinsulin-like growth factor II; D007335:Insulin-Like Growth Factor II