The trans-sialidase of Trypanosoma cruzi is anchored by two different lipids.
The trans-sialidase from the trypomastigote stage of Trypanosoma cruzi was metabolically labeled with [3H]-palmitic acid and purified by immunoprecipitation with a monoclonal antibody. The action of PI-PLC on the immunoprecipitate released a lipid that was analyzed by TLC. Lyso-1-O-hexadecylglycerol and N-palmitoyl-sphinganine were obtained in a 1:3 ratio. A comparison with the GPI anchors present in the different stages of T. cruzi was made.
Agusti
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CIHIDECAR, Departmento de Química Orgánica, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Argentina.
Couto
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Campetella
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Frasch
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de Lederkremer
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D000818:Animals; D002855:Chromatography, Thin Layer; D017261:Glycosylphosphatidylinositols / Q000378:metabolism*; D008563:Membrane Lipids / Q000378:metabolism*; D051379:Mice; D009439:Neuraminidase / Q000378:metabolism*; D043265:Phosphatidylinositol Diacylglycerol-Lyase; D054801:Phosphoinositide Phospholipase C; D013379:Substrate Specificity; D014349:Trypanosoma cruzi / Q000201:enzymology*; D010738:Type C Phospholipases / Q000378:metabolism
D017261:Glycosylphosphatidylinositols; D008563:Membrane Lipids; D010738:Type C Phospholipases; D054801:Phosphoinositide Phospholipase C; D009439:Neuraminidase; D043265:Phosphatidylinositol Diacylglycerol-Lyase