title: Differential expression of calretinin, calbindin D28K and parvalbumin in the developing human cerebellum.
abstract: Three calcium-binding proteins, calretinin, calbindin D28K and parvalbumin, were immunohistochemically localized in the human cerebellum at different developmental stages. Cells positive for calretinin were not detected during early development of the cerebellum until 21 weeks of gestation at which stage weak staining was found in Purkinje and basket cells of the cortex and in neurons of the dentate nucleus. Both the number of positive cells and the intensity of immunoreactivities were found to increase as the cerebellum became more mature. Calbindin D28K immunoreactivity was, however, detected early in development at 14 weeks of gestation. Positive cells were found in Purkinje, basket, stellate and granule cells of the cerebellar cortex and in neurons of fastigial, globose, emboliform and dentate nuclei. The number of positive cells and the staining intensity for calbindin in both the cerebellar cortex and deep nuclei decreased at more advanced developmental stages. At 21-31 weeks of gestation, positive staining was restricted to Purkinje and basket cells of the cortex. Parvalbumin immunoreactivity was also observed early in development at 14 weeks of gestation. Positivity was found in Purkinje, basket and stellate cells of the cerebellar cortex and in neurons of all the deep nuclei, with the highest number of positive cells in the fastigial nucleus followed by emboliform, globose and dentate nuclei. As the cerebellum became more mature, both the number of positive cells and the staining intensity for parvalbumin decreased in the cortex and deep nuclei. The results of the present study showed that among the three calcium-binding proteins examined, strong immunoreactivities for calbindin D28K and parvalbumin were found inthe human cerebellum early in development at 14 weeks of gestation, but there was a decrease in both the intensity and number of positive cells at more advanced stages. In contrast, calretinin positive cells were not detected until 21 weeks of gestation and the immunoreactivity increased as the cerebellum became more mature. A possible correlation between the developmentally regulated expression of the calcium-binding proteins and expression of different neurotransmitters during development is discussed.
authors.affiliation: Department of Anatomy, Faculty of Medicine, Chinese University of Hong Kong, Shatin, Hong Kong.
authors.forename: D T
authors.initials: DT
authors.lastname: Yew
authors.forename: C B
authors.initials: CB
authors.lastname: Luo
authors.forename: C W
authors.initials: CW
authors.lastname: Heizmann
authors.forename: W Y
authors.initials: WY
authors.lastname: Chan
mesh_terms: D000028:Abortion, Induced; D064092:Calbindin 1; D064032:Calbindin 2; D064026:Calbindins; D002529:Cerebellar Nuclei / Q000254:growth & development / Q000378:metabolism; D002531:Cerebellum / Q000196:embryology* / Q000378:metabolism; D005314:Embryonic and Fetal Development; D018507:Gene Expression Regulation, Developmental; D005865:Gestational Age; D006801:Humans; D009419:Nerve Tissue Proteins / Q000032:analysis / Q000096:biosynthesis; D009474:Neurons / Q000166:cytology / Q000378:metabolism; D010320:Parvalbumins / Q000032:analysis / Q000096:biosynthesis*; D011689:Purkinje Cells / Q000166:cytology / Q000378:metabolism; D064030:S100 Calcium Binding Protein G / Q000032:analysis / Q000096:biosynthesis*
chemical_list: C576498:CALB1 protein, human; C576764:CALB2 protein, human; D064092:Calbindin 1; D064032:Calbindin 2; D064026:Calbindins; D009419:Nerve Tissue Proteins; D010320:Parvalbumins; D064030:S100 Calcium Binding Protein G