Determination of noscapine in human plasma using solid-phase extraction and high-performance liquid chromatography.
A simple, reliable high-performance liquid chromatographic (HPLC) method has been developed and validated for the determination of noscapine in human plasma. Noscapine and the internal standard, papaverine were quantitatively extracted from plasma onto disposable extraction columns by means of an automated off-line solid-phase extraction system and were separated onto a reversed-phase column. The method was found to be precise and accurate within the range 7.2-270 ng/ml. No endogenous compounds interfered with the assay.
Chollet
D F
DF
Covance Central Laboratory Services SA, Bioanalytical Division, Meyrin/Geneva, Switzerland.
Ruols
C
C
Arnera
V
V
D000996:Antitussive Agents / Q000097:blood*; D002851:Chromatography, High Pressure Liquid; D006801:Humans; D009665:Noscapine / Q000097:blood*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
D000996:Antitussive Agents; D009665:Noscapine