Molecular characterization of a novel fission yeast gene spUAP2 that interacts with the splicing factor spU2AF59.
A protein essential for pre-mRNA splicing, the U2 auxiliary factor (U2AF), is composed of a large and small subunit. Previously we cloned and characterized both subunits, spU2AF59 and spU2AF23, from fission yeast. We now report a novel U2AF-associated-protein, spUAP2, which interacts with both subunits. SpUAP2 contains a classical and a degenerate RNA recognition motif (RRM), both of which are required for interaction with spU2AF59. Interaction also requires the arginine/serine-rich region and the first RRM of spU2AF59. A null allele of the gene for spUAP2 is lethal.
McKinney
R
R
Department of Pharmacology and Molecular Biology, Finch University of Health Sciences, The Chicago Medical School, 3333 Green Bay Road, North Chicago, IL 60064, USA.
Wentz-Hunter
K
K
Schmidt
H
H
Potashkin
J
J
D000595:Amino Acid Sequence; D001483:Base Sequence; D001665:Binding Sites / Q000235:genetics; D017931:DNA Primers / Q000235:genetics; D004271:DNA, Fungal / Q000235:genetics; D005656:Fungal Proteins / Q000235:genetics* / Q000378:metabolism; D005800:Genes, Fungal; D008969:Molecular Sequence Data; D009687:Nuclear Proteins; D012326:RNA Splicing; D012331:RNA, Fungal / Q000235:genetics / Q000378:metabolism; D016601:RNA-Binding Proteins; D012261:Ribonucleoproteins / Q000378:metabolism*; D012441:Saccharomyces cerevisiae / Q000235:genetics; D012568:Schizosaccharomyces / Q000235:genetics* / Q000378:metabolism*; D029702:Schizosaccharomyces pombe Proteins; D017386:Sequence Homology, Amino Acid; D000072668:Splicing Factor U2AF
D017931:DNA Primers; D004271:DNA, Fungal; D005656:Fungal Proteins; D009687:Nuclear Proteins; D012331:RNA, Fungal; D016601:RNA-Binding Proteins; D012261:Ribonucleoproteins; D029702:Schizosaccharomyces pombe Proteins; D000072668:Splicing Factor U2AF; C109989:UAP2 protein, S pombe