Purification and characterization of transcription factor IIIA from higher plants.
Transcription factor IIIA (TF IIIA) binds and specifically activates transcription of eukaryotic 5S rRNA genes. It also forms a 7S ribonucleoprotein complex with mature 5S rRNA. Here, we describe the purification and properties of pTF IIIA from higher plants. The purified protein from tulip (Tulipa whittalii) has a molecular mass of about 40 kDa and also binds 5S rRNA and 5S rRNA genes. pTF IIIA also facilitates the transcription of a 5S rRNA gene in a HeLa cell extract.
Wyszko
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Institute of Bioorganic Chemistry, Polish Academy of Sciences, Noskowskiego, Poznań.
Barciszewska
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D018744:DNA, Plant / Q000235:genetics / Q000378:metabolism; D004275:DNA, Ribosomal / Q000235:genetics / Q000378:metabolism; D004268:DNA-Binding Proteins / Q000302:isolation & purification* / Q000378:metabolism*; D017343:Genes, Plant; D006367:HeLa Cells; D006801:Humans; D008970:Molecular Weight; D010940:Plant Proteins / Q000302:isolation & purification / Q000378:metabolism; D010944:Plants / Q000235:genetics / Q000378:metabolism*; D018749:RNA, Plant / Q000235:genetics / Q000378:metabolism; D012341:RNA, Ribosomal, 5S / Q000235:genetics / Q000378:metabolism; D012261:Ribonucleoproteins / Q000378:metabolism; D035582:Transcription Factor TFIIIA; D014157:Transcription Factors / Q000302:isolation & purification* / Q000378:metabolism*; D014158:Transcription, Genetic
D018744:DNA, Plant; D004275:DNA, Ribosomal; D004268:DNA-Binding Proteins; D010940:Plant Proteins; D018749:RNA, Plant; D012341:RNA, Ribosomal, 5S; D012261:Ribonucleoproteins; D035582:Transcription Factor TFIIIA; D014157:Transcription Factors