title: Allatostatin in hemocytes of the cockroach Diploptera punctata.
abstract: Allatostatins are neuropeptides that inhibit the production, by the corpora allata, of a major insect hormone, juvenile hormone. These peptides are produced by cells of the brain and ganglia as well as by midgut endocrine cells. Transport from these sites may contribute to the allatostatin content in the hemolymph (insect blood). Using a monoclonal antibody against Diploptera punctata allatostatin I (A-P-S-G-A-Q-R-L-Y-G-F-G-L-NH2) and in situ hybridization with a digoxigenin-labeled cRNA probe generated from a portion of the allatostatin gene, it is demonstrated that allatostatin is present in and synthesized by granular hemocytes of D. punctata. About 5% of the hemocytes react with anti-allatostatin antibody and a similar number hybridize with a cRNA probe that detects allatostatin-specific mRNA. Electron micrographs showed that allatostatin-immunoreactive material occurs in membrane-bound, uniformly dense granules that frequently fill fusiform-shaped cells. Allatostatin in cell and plasma fractions of hemolymph quantified by enzyme-linked immunosorbent assay and by bioassay for inhibition of juvenile hormone synthesis in vitro indicated that about equal quantities (0.1-0.2 fmol/microl) are present in cell and plasma fractions. The production of allatostatin by hemocytes suggests that allatostatins may function as regulatory peptides in hemolymph activities in addition to their other known functions.
authors.affiliation: Department of Biological Sciences, University of Iowa, Iowa City, IA 52242, USA.
authors.forename: J R
authors.initials: JR
authors.lastname: Skinner
authors.forename: S E
authors.initials: SE
authors.lastname: Fairbairn
authors.forename: A P
authors.initials: AP
authors.lastname: Woodhead
authors.forename: W G
authors.initials: WG
authors.lastname: Bendena
authors.forename: B
authors.initials: B
authors.lastname: Stay
mesh_terms: D000818:Animals; D003058:Cockroaches / Q000502:physiology*; D003594:Cytoplasmic Granules / Q000737:chemistry / Q000648:ultrastructure; D005260:Female; D006434:Hemocytes / Q000737:chemistry* / Q000276:immunology / Q000648:ultrastructure; D006458:Hemolymph / Q000737:chemistry; D006727:Hormone Antagonists / Q000032:analysis* / Q000378:metabolism; D007150:Immunohistochemistry; D017403:In Situ Hybridization; D008297:Male; D008854:Microscopy, Electron; D009479:Neuropeptides / Q000032:analysis* / Q000235:genetics; D010949:Plasma / Q000737:chemistry; D012333:RNA, Messenger / Q000032:analysis
chemical_list: D006727:Hormone Antagonists; D009479:Neuropeptides; D012333:RNA, Messenger; C076523:allatostatin