Two novel members of the prolactin/growth hormone family are expressed in the mouse placenta.
Two novel members of the mouse PRL/GH family have been identified through a search of an expressed sequence tag database. The encoded proteins do not appear to be homologs of other known members of this hormone family. One of these proteins, designated PRL-like protein E (PLP-E), is predicted to be synthesized as a precursor of 265 amino acids, modified by N-linked glycosylation, and secreted as a mature glycoprotein of 236 residues. The second clone encodes a protein of 253 residues with consensus sites for N-linked glycosylation; the secreted form of the protein, designated PRL-like protein F (PLP-F), is predicted to be 223 amino acids in length. Both of these messenger RNAs are expressed specifically in the placenta, with peak levels of PLP-E on days 10-12 and of PLP-F on days 14-16. Expression of PLP-E is restricted to the trophoblast giant cells, whereas PLP-F is synthesized only in the spongiotrophoblasts. The genes for both of these proteins map to a 700-kilobase region of mouse chromosome 13 that includes other members of the PRL/GH family.
Lin
J
J
Department of Biochemistry, Molecular Biology, and Cell Biology, Northwestern University, Evanston, Illinois 60208, USA.
Poole
J
J
Linzer
D I
DI
D000595:Amino Acid Sequence; D000818:Animals; D002874:Chromosome Mapping; D016207:Cytokines / Q000378:metabolism*; D018076:DNA, Complementary / Q000235:genetics; D005260:Female; D051379:Mice; D008969:Molecular Sequence Data; D010920:Placenta / Q000378:metabolism*; D010926:Placental Hormones / Q000378:metabolism*; D011247:Pregnancy; D011257:Pregnancy Proteins / Q000378:metabolism*
D016207:Cytokines; D018076:DNA, Complementary; D010926:Placental Hormones; D011257:Pregnancy Proteins; C423011:Prlpe protein, mouse