Genotypic relationships among strains classified under the (Pasteurella) haemolytica-complex as indicated by ribotyping and multilocus enzyme electrophoresis.
Two-hundred and one strains classified under the (Pasteurella) haemolytica-complex isolated from cattle, sheep, deer, pigs, hares and rabbits were investigated by ribotyping. Fifty-nine of these strains were selected for further studies using multilocus enzyme electrophoresis (MEE). A correlation between the clusters identified by ribotyping and MEE was demonstrated and the results furthermore indicated that a genetic basis exists for most clusters previously outlined by the use of quantitative evaluation of phenotypic data. The taxonomic relevance of ornithine decarboxylase and fermentation of L-arabinose, D-sorbitol and glucosides for taxonomic delineation within the (P.) haemolytica-complex was supported. A taxonomic importance was further indicated for ONPG, ONPX, ONPF, meso-inositol, D-xylose, maltose, dextrine and NPG in relation to some of the taxa. Within the porcine taxon 15, however, differences in ornithine decarboxylase did not correspond to genetic clusters. Six lineages were revealed by MEE. Lineage A contained electrophoretic types (ETs) representing biogroups 1, 3A-3H, 8A and 9, indicating a genetic relationship between these groups--an observation which was supported by ribotyping. Lineage B included biogroup 8D, 3 strains from biogroup 10 and a single strain from biogroup 1 and taxon 18/biovar 1. Lineage C contained strains allocated to biogroup 6 from ruminants and the porcine taxon 15. The similarity between these two groups was accentuated by ribotyping. Lineage D and the single isolate in lineage E contained strains allocated to biogroups 7, 10, 8B and 8C, in addition to single strains from biogroups 6 and 9. The same strains were found in the heterogenous ribotype cluster 17. Lineage F contained strains representing the leprine taxon 20 and the ruminant (P.) granulomatis. Ribotyping indicated that the ruminant biogroup 3J was affiliated with both taxon 20 and (P.) granulomatis.
Angen
O
O
Department of Veterinary Microbiology, Royal Veterinary and Agricultural University, Frederiksberg C, Denmark. ang@svs.dk
Caugant
D A
DA
Olsen
J E
JE
Bisgaard
M
M
D000483:Alleles; D000818:Animals; D001089:Arabinose / Q000378:metabolism; D002417:Cattle; D004269:DNA, Bacterial / Q000032:analysis*; D003670:Deer; D004798:Enzymes / Q000032:analysis*; D005960:Glucosides / Q000378:metabolism; D007294:Inositol / Q000378:metabolism; D016978:Mannheimia haemolytica / Q000145:classification* / Q000201:enzymology / Q000235:genetics; D009693:Nucleic Acid Hybridization; D009955:Ornithine Decarboxylase / Q000235:genetics; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics*; D012338:RNA, Ribosomal, 23S / Q000235:genetics*; D011817:Rabbits; D012756:Sheep; D013012:Sorbitol / Q000378:metabolism; D013552:Swine; D014994:Xylose / Q000378:metabolism; D014995:Xylosidases / Q000378:metabolism; D005644:alpha-L-Fucosidase / Q000378:metabolism; D001616:beta-Galactosidase / Q000378:metabolism
D004269:DNA, Bacterial; D004798:Enzymes; D005960:Glucosides; D012336:RNA, Ribosomal, 16S; D012338:RNA, Ribosomal, 23S; D007294:Inositol; D013012:Sorbitol; D014994:Xylose; D001089:Arabinose; D014995:Xylosidases; D001616:beta-Galactosidase; C026579:exo-1,4-beta-D-xylosidase; D005644:alpha-L-Fucosidase; D009955:Ornithine Decarboxylase