[DNA contents and cycle analysis of porcine burn wounds treated with growth factors].
Wound healing is a complex biologic process in which peptide growth factors play a very important role. The object of this research was to study the effects of recombinant human epidermal growth factors (rh-EGF) and acidic fibroblast growth factor (aFGF), basic fibroblast growth factor (bFGF) and bovine brain extract (bBE) on porcine partial-thickness burn wound healing. The biopsies were taken for determination of DNA contents and cell DNA cycle with the aid of flow cytometry and histological observation. The results showed that more extensive reepithelization and greater thickness of epidermis exhibited in growth factor treated wounds, and DNA contents and percent of S phase of cell DNA cycle were also higher in those groups treated with growth factors. The results suggested that repeated topical application of rh-EGF, aFGF, bFGF and bBE accelerated epithelial healing of burn wounds, and it may be helpful in clinical practice.
Pan
Y
Y
Burn Center, Changhai Hospital, Second Military Medical University, Shanghai.
Dai
F
F
Chen
Y
Y
D000818:Animals; D002056:Burns / Q000378:metabolism / Q000628:therapy*; D004247:DNA / Q000032:analysis*; D004815:Epidermal Growth Factor / Q000627:therapeutic use*; D016220:Fibroblast Growth Factor 1 / Q000627:therapeutic use; D016222:Fibroblast Growth Factor 2 / Q000627:therapeutic use; D011994:Recombinant Proteins / Q000627:therapeutic use; D013552:Swine; D014020:Tissue Extracts / Q000627:therapeutic use; D014945:Wound Healing / Q000187:drug effects
D011994:Recombinant Proteins; D014020:Tissue Extracts; D016222:Fibroblast Growth Factor 2; D016220:Fibroblast Growth Factor 1; D004815:Epidermal Growth Factor; D004247:DNA