title: The N-acetylgalactosamine and lactosamine specific lectin from Iris hybrida leaves.
abstract: The lectin isolated from the leaves of Iris hybrida binds specifically N-acetyl-galactosamine and lactose. Its molecule consists of two identical subunits bound by disulfide bonds. The lectin is a glycoprotein containing about 12% of sugars. It binds asialoglycoproteins containing complex type sugar chains. The binding is reduced by half at the concentration of 0.15 to 0.40 mM of the galactose containing disaccharides irrespectively to a type of galactose isomer. This indicates rather broad specificity of I. hybrida leaf lectin.
authors.affiliation: Institute of Biochemistry and Molecular Biology, University of Wrocław, Poland.
authors.forename: M
authors.initials: M
authors.lastname: Ferens-Sieczkowska
authors.forename: M
authors.initials: M
authors.lastname: Orczyk-Pawiłowicz
authors.forename: B
authors.initials: B
authors.lastname: Morawiecka
mesh_terms: D000116:Acetylgalactosamine / Q000378:metabolism*; D000606:Amino Sugars / Q000378:metabolism*; D004187:Disaccharides / Q000378:metabolism; D004586:Electrophoresis; D005690:Galactose / Q000378:metabolism; D006023:Glycoproteins / Q000378:metabolism; D037102:Lectins / Q000032:analysis / Q000302:isolation & purification* / Q000378:metabolism*; D018515:Plant Leaves / Q000737:chemistry / Q000378:metabolism; D037121:Plant Lectins; D018517:Plant Roots / Q000737:chemistry / Q000378:metabolism; D010944:Plants / Q000737:chemistry* / Q000378:metabolism; D013379:Substrate Specificity
chemical_list: D000606:Amino Sugars; D004187:Disaccharides; D006023:Glycoproteins; D037102:Lectins; D037121:Plant Lectins; C032233:lactosamine; D000116:Acetylgalactosamine; D005690:Galactose