Detection via laser desorption and mass spectrometry of multiplex electrophore-labeled albumin.
Albumin was reacted with a mixture of six electrophore N-hydroxysuccinimide esters, each of which possessed an interior glycolketo linkage. The purpose of this linkage is to release the attached electrophore as a ketone when heated, due to a thermal retro-aldol reaction. The multiplex electrophore-labeled albumin was detected as a dried spot deposited on a polyimide membrane by laser desorption/capillary collection (of the released ketone electrophores)/off-line gas chromatography/electron capture-mass spectrometry. This encourages further study of such electrophore labels in immunoassays and related techniques, where there is a need to make advances in multi-analyte detection.
Bian
N
N
Department of Pharmaceutical Sciences, Bouvé College of Pharmacy and Health Professions, Barnett Institute, Northeastern University, Boston, MA 02115, USA.
Wang
P
P
Wang
Z
Z
Xu
L
L
Church
G
G
Giese
R W
RW
D000418:Albumins / Q000032:analysis*; D008401:Gas Chromatography-Mass Spectrometry; D007834:Lasers; D013058:Mass Spectrometry; D012997:Solvents
D000418:Albumins; D012997:Solvents