Identification of an achaete-scute homolog, Fash1, from Fugu rubripes.
Proteins of the achaete-scute family of transcription factors play important roles in neurogenesis in both invertebrates and vertebrates. Here, we report the cloning and characterization of a Japanese pufferfish, Fugu rubripes achaete-scute homolog 1, Fash1. Sequence alignment of the predicted amino acid sequence of Fash1 with other vertebrate homologs of the achaete scute homolog 1 subclass shows that the carboxyl 2/3 of the protein, including the basic helix-loop-helix, a putative nuclear localization signal and several consensus phosphorylation sites, is highly conserved. Strikingly, the similarity in this region between eight vertebrate species is close to 90%.
Verma-Kurvari
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S
Cell Biology and Neuroscience, University of Texas Southwestern Medical Center, Dallas 75235-9111, USA.
Johnson
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JE
D000595:Amino Acid Sequence; D000818:Animals; D051792:Basic Helix-Loop-Helix Proteins; D016384:Consensus Sequence; D004268:DNA-Binding Proteins / Q000096:biosynthesis / Q000737:chemistry* / Q000235:genetics*; D029721:Drosophila Proteins; D029941:Fish Proteins; D005400:Fishes, Poisonous / Q000235:genetics*; D018257:Helix-Loop-Helix Motifs; D006801:Humans; D007448:Invertebrates; D051379:Mice; D008969:Molecular Sequence Data; D010766:Phosphorylation; D051381:Rats; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid; D014157:Transcription Factors / Q000096:biosynthesis / Q000737:chemistry* / Q000235:genetics*; D014714:Vertebrates; D014981:Xenopus
D051792:Basic Helix-Loop-Helix Proteins; D004268:DNA-Binding Proteins; D029721:Drosophila Proteins; D029941:Fish Proteins; D014157:Transcription Factors; C109105:Fash1 protein, Takifugu rubripes; C089199:ac protein, Drosophila; C089197:sc protein, Drosophila