title: Electrocochleographic evaluation of the guinea pig model of endolymphatic hydrops.
abstract: Electrocochleography (ECochG) was used to evaluate cochlear function in guinea pigs with experimentally induced endolymphatic hydrops (ELH) before and after osmotic dehydration with either glycerol or urea. We surgically induced ELH in the right ears of 9 guinea pigs, while the right ears of 6 guinea pigs received a sham operation. The left ears of the 15 animals constituted the normal group. Eight weeks after surgery, summating potential (SP) and action potential (AP) amplitudes were measured prior to and following the administration of glycerol or urea. The SPs and SP/AP ratios were reduced in all groups, with no significant differences among groups or between dehydrating agents. Some of the hydropic ears, however, did show an increased AP threshold and a recruitment effect. In measurements from 6 additional animals, serum osmolarity increased more with urea than with glycerol. The guinea pig model remains valuable for investigation of ELH, even though it differs in significant respects from ELH in humans.
authors.affiliation: Department of Otorhinolaryngology, University of Texas Southwestern Medical Center at Dallas, 75235-9035, USA.
authors.forename: M
authors.initials: M
authors.lastname: Badr-El-Dine
authors.forename: G M
authors.initials: GM
authors.lastname: Gerken
authors.forename: C G
authors.initials: CG
authors.lastname: Wright
authors.forename: K S
authors.initials: KS
authors.lastname: Robinson
authors.forename: W L
authors.initials: WL
authors.lastname: Meyerhoff
mesh_terms: D000200:Action Potentials; D000818:Animals; D001300:Audiometry, Evoked Response / Q000379:methods*; D001309:Auditory Threshold; D003681:Dehydration / Q000150:complications; D004195:Disease Models, Animal; D018159:Endolymphatic Hydrops / Q000139:chemically induced / Q000175:diagnosis*; D005260:Female; D005990:Glycerol; D006168:Guinea Pigs; D008297:Male; D008575:Meniere Disease / Q000503:physiopathology; D009994:Osmolar Concentration; D011999:Recruitment, Neurophysiological; D015203:Reproducibility of Results; D014508:Urea
chemical_list: D014508:Urea; D005990:Glycerol