Regulation of CD44 in the regenerating mouse facial motor nucleus.
CD44 is a cell adhesion molecule which plays an important role in cell movement and adhesion, e.g. in lymphocyte homing and tumour metastasis. Here we studied the expression of CD44 mRNA and protein immunoreactivity in the facial nucleus after nerve injury and during the ensuing regeneration. Transection of the facial nerve led to a strong up-regulation of CD44, peaking 4 days after injury on the motoneurons of the axotomized facial nucleus. Use of the polymerase chain reaction confirmed the de novo expression of CD44 and detected only the standard haematopoietic CD44 isoform. Western blotting also detected the 76 kDa protein subtype, in line with the predicted size of the haematopoietic CD44 variant. At the ultrastructural level, CD44 immunoreactivity was restricted to the surface of the neuronal perikarya, their dendrites and axons. It was not seen in the adjacent activated astrocytes, microglia or vascular endothelia. This study shows strong up-regulation of the cell adhesion molecule CD44 on the regenerating motoneurons in the axotomized facial nucleus. These data suggest that CD44 may play a role in neurite outgrowth, in synaptic stripping or in the adhesion of activated glial cells to the perikaryal surface of the axotomized motoneurons.
Jones
L L
LL
Department of Neuromorphology, Max Planck Institute of Psychiatry, Martinsried, Germany.
Kreutzberg
G W
GW
Raivich
G
G
D017398:Alternative Splicing; D000818:Animals; D000918:Antibody Specificity; D015153:Blotting, Western; D002478:Cells, Cultured; D005154:Facial Nerve / Q000502:physiology*; D020220:Facial Nerve Injuries; D005260:Female; D018960:Hyaluronan Receptors / Q000276:immunology*; D007150:Immunohistochemistry; D051379:Mice; D016253:Microscopy, Immunoelectron; D009046:Motor Neurons / Q000502:physiology*; D009416:Nerve Regeneration / Q000502:physiology*; D016133:Polymerase Chain Reaction; D051381:Rats; D017208:Rats, Wistar
D018960:Hyaluronan Receptors