Isolation and characterization of cytosolic copper/zinc superoxide dismutase from Capsicum annuum L.
A cDNA clone for a cytosolic Cu/Zn superoxide dismutase (Cu/ZnSOD) was isolated and characterized from red pepper (Capsicum annuum L.). The clone consisted of 735 bp containing one open reading frame (ORF) of 459 bp, 46 bp of 5'- and 230 bp of 3'-untranslated region. The nucleotide sequence of the ORF showed 93% homology with that of Nicotiana plumbaginifolia and tomato. It encodes a polypeptide of 154 amino acids with a molecular weight of 15,300. Genomic Southern hybridization suggested that only one copy is present. During cold treatment at 4 degrees C, its expression was maintained at a similar level regardless of illumination.
Kim
Y K
YK
Department of Biology, Seoul National University, Korea.
Kwon
S I
SI
An
C S
CS
D000595:Amino Acid Sequence; D001483:Base Sequence; D015139:Blotting, Southern; D002212:Capsicum / Q000201:enzymology* / Q000235:genetics; D003001:Cloning, Molecular; D003080:Cold Temperature; D003300:Copper; D003600:Cytosol / Q000201:enzymology*; D018076:DNA, Complementary / Q000302:isolation & purification; D018744:DNA, Plant / Q000032:analysis; D007527:Isoenzymes / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification; D008969:Molecular Sequence Data; D010940:Plant Proteins / Q000096:biosynthesis / Q000737:chemistry* / Q000235:genetics / Q000302:isolation & purification*; D010946:Plants, Medicinal; D017422:Sequence Analysis, DNA; D013482:Superoxide Dismutase / Q000096:biosynthesis / Q000737:chemistry* / Q000235:genetics / Q000302:isolation & purification*; D015032:Zinc
D018076:DNA, Complementary; D018744:DNA, Plant; D007527:Isoenzymes; D010940:Plant Proteins; D003300:Copper; D013482:Superoxide Dismutase; D015032:Zinc