title: The use of capillary electrophoresis for point-mutation screening.
abstract: Advances in capillary electrophoresis technology over the past three years have been rapid. Capillary electrophoresis offers high-throughput, high-resolution, automatic operation and on-line detection with automatic data acquisition, and this has stimulated its application to the analysis of DNA mutations. Many different PCR-based DNA-mutation assays have been developed for unknown and known mutations. This article compares conventional PCR-based mutation-detection assays with the methods developed for use with capillary electrophoresis. Future trends for mutation detection using capillary electrophoresis are also assessed, with a special emphasis on totally integrated, microchip capillary-electrophoresis-based mutation-detection systems.
authors.affiliation: ForBio Research Pty Ltd, Indooroopilly, Australia.
authors.forename: K R
authors.initials: KR
authors.lastname: Mitchelson
authors.forename: J
authors.initials: J
authors.lastname: Cheng
authors.forename: L J
authors.initials: LJ
authors.lastname: Kricka
mesh_terms: D004247:DNA; D019075:Electrophoresis, Capillary / Q000379:methods*; D017354:Point Mutation; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic
chemical_list: D004247:DNA