A novel wound dressing with an antibiotic delivery system stimulated by microbial infection.
The aim of this study was to develop a new wound dressing with controlled release of antibiotics only in the presence of infection. In the first experiment using an infected dorsal pouch of rats, exudate containing proteinases from pouches contaminated with Staphylococcus aureus or Pseudomonas aeruginosa showed significantly higher hydrolytic activity toward Boc-Val-Pro-Arg-MCA than that from noninfected wounds. The authors then developed a new type of wound dressing (AGX), a drug delivery system in which gentamicin is bound to polyvinylalcohol hydrogel through an enzymatically degradable peptide linker containing a -(D)-Phe-Pro-Arg-sequence. To investigate in vitro effectiveness, AGX was incubated with exudate from S. aureus infected or P. aeruginosa infected wounds. Gentamicin was selectively released from AGX in the presence of the exudate from S. aureus infected or P. aeruginosa infected wounds, but was not released in the presence of exudate from noninfected wounds. Next, AGX or the polyvinylalcohol hydrogel that served as control was incubated with S. aureus in the presence of human plasma. After 24 hours, S. aureus concentration was markedly lower in the case with AGX than in that with polyvinylalcohol hydrogel. These results indicate that proteinases from wounds infected with S. aureus or P. aeruginosa cleaved the linker and gentamicin was released.
Suzuki
Y
Y
Department of Plastic and Reconstructive Surgery, Faculty of Medicine, Kyoto University, Japan.
Tanihara
M
M
Nishimura
Y
Y
Suzuki
K
K
Kakimaru
Y
Y
Shimizu
Y
Y
D000818:Animals; D000900:Anti-Bacterial Agents / Q000008:administration & dosage*; D001458:Bandages; D016503:Drug Delivery Systems; D005069:Evaluation Studies as Topic; D005122:Exudates and Transudates / Q000378:metabolism; D005782:Gels; D005839:Gentamicins / Q000008:administration & dosage; D006801:Humans; D066298:In Vitro Techniques; D008297:Male; D011142:Polyvinyl Alcohol; D011552:Pseudomonas Infections / Q000188:drug therapy / Q000628:therapy; D051381:Rats; D017208:Rats, Wistar; D013203:Staphylococcal Infections / Q000188:drug therapy / Q000628:therapy; D013917:Thrombin / Q000378:metabolism; D014946:Wound Infection / Q000188:drug therapy* / Q000378:metabolism / Q000628:therapy
D000900:Anti-Bacterial Agents; D005782:Gels; D005839:Gentamicins; D011142:Polyvinyl Alcohol; D013917:Thrombin