Identification and analysis of a novel member of the ubiquitin family expressed in dendritic cells and mature B cells.
Using a cDNA subtraction technique, a novel member of the ubiquitin family was isolated from human dendritic cells. This gene encodes a diubiquitin protein containing tandem head to tail ubiquitin-like domains, with the conservation of key functional residues. Expression of this 777-bp mRNA was restricted to dendritic cells and B cells, with strong expression in mature B cells. Southern blot analysis indicated that a single copy of this gene is present. In situ hybridization on tonsillar tissue showed expression in epithelial cells and isolated cells within the germinal center. With respect to an expressed-sequence tag (EST) this cDNA could be localized to the major histocompatibility complex class I region of chromosome 6. Comparative analysis and the expression pattern of this gene suggests a function in antigen processing and presentation.
Bates
E E
EE
Schering-Plough, Laboratory for Immunological Research, Dardilly, France.
Ravel
O
O
Dieu
M C
MC
Ho
S
S
Guret
C
C
Bridon
J M
JM
Ait-Yahia
S
S
Brière
F
F
Caux
C
C
Banchereau
J
J
Lebecque
S
S
D000595:Amino Acid Sequence; D001402:B-Lymphocytes / Q000378:metabolism*; D001483:Base Sequence; D002454:Cell Differentiation; D016384:Consensus Sequence; D018076:DNA, Complementary / Q000235:genetics; D003713:Dendritic Cells / Q000378:metabolism*; D005312:Fetal Blood / Q000166:cytology; D015870:Gene Expression; D005796:Genes; D006801:Humans; D008969:Molecular Sequence Data; D014066:Palatine Tonsil / Q000166:cytology; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000096:biosynthesis; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid; D013382:Subtraction Technique; D014452:Ubiquitins / Q000096:biosynthesis / Q000235:genetics / Q000302:isolation & purification*
D018076:DNA, Complementary; D012333:RNA, Messenger; C109184:UBD protein, human; D014452:Ubiquitins