title: Seminal vesiculitis and orchitis caused by Brucella abortus biovar 1 in young bison bulls from South Dakota.
abstract: Specimens of blood, lymph nodes, spleens, and genitalia were collected at slaughter from seven 3- and 4-year-old male bison that had recently become seropositive for brucellosis. The animals were from a captive herd of approximately 3,500 bison located in central South Dakota. Brucella abortus biovar 1 was isolated from 2 or more specimens from each of 6 bison. Severe necrotizing and pyogranulomatous orchitis was present in 1 testicle from 1 bull, and 4 animals had mild to marked seminal vesiculitis. Immunohistochemical staining labeled organisms in seminal vesicles and the testicle with orchitis. Ultrastructurally, intact bacilli were present in cytoplasmic vacuoles of some macrophages; other macrophages contained intracytoplasmic aggregates of calcified coccobacilli.
authors.affiliation: Pathobiology Laboratory, U.S. Department of Agriculture, Ames, IA 50010, USA.
authors.forename: J C
authors.initials: JC
authors.lastname: Rhyan
authors.forename: S D
authors.initials: SD
authors.lastname: Holland
authors.forename: T
authors.initials: T
authors.lastname: Gidlewski
authors.forename: D A
authors.initials: DA
authors.lastname: Saari
authors.forename: A E
authors.initials: AE
authors.lastname: Jensen
authors.forename: D R
authors.initials: DR
authors.lastname: Ewalt
authors.forename: S G
authors.initials: SG
authors.lastname: Hennager
authors.forename: S C
authors.initials: SC
authors.lastname: Olsen
authors.forename: N F
authors.initials: NF
authors.lastname: Cheville
mesh_terms: D000038:Abscess / Q000382:microbiology / Q000473:pathology / Q000662:veterinary; D000818:Animals; D000942:Antigens, Bacterial / Q000032:analysis; D016164:Bison; D002003:Brucella abortus / Q000302:isolation & purification; D002006:Brucellosis / Q000150:complications / Q000473:pathology / Q000662:veterinary*; D005832:Genital Diseases, Male / Q000382:microbiology / Q000473:pathology / Q000662:veterinary*; D007124:Immunoenzyme Techniques; D008264:Macrophages / Q000382:microbiology / Q000473:pathology / Q000648:ultrastructure; D008297:Male; D009920:Orchitis / Q000382:microbiology / Q000473:pathology / Q000662:veterinary*; D012669:Seminal Vesicles / Q000382:microbiology / Q000473:pathology*; D013023:South Dakota; D013737:Testis / Q000382:microbiology / Q000473:pathology
chemical_list: D000942:Antigens, Bacterial