[Study on the distribution and origins of RNA in the metaphase chromosomes of mouse by in situ hybridization at electron microscopic level].
The RNAs in the metaphase chromosomes of mouse are located by use of RNase-gold labelling. The result shows that a lot of RNAs distribute uniformly in the inner and peripheral regions of the chromosome sections. Moreover, the distributions of 18 s rRNA and 5 s rRNA which are studied under the electron microscope by in situ hybridization indicate that 18 s rRNA and 5 s rRNA are two among the several origins of the inner RNAs and peripheral RNAs in mouse metaphase chromosomes.
Mei
Y
Y
Biochem and Molecular Bio Dept., Nankai University, Tianjin.
Zhang
Z L
ZL
Fan
T Y
TY
Guo
S Y
SY
Wang
X L
XL
D000818:Animals; D002466:Cell Nucleolus / Q000737:chemistry; D002875:Chromosomes / Q000235:genetics* / Q000648:ultrastructure; D017403:In Situ Hybridization; D008677:Metaphase; D051379:Mice; D008854:Microscopy, Electron; D012337:RNA, Ribosomal, 18S / Q000032:analysis*; D012341:RNA, Ribosomal, 5S / Q000032:analysis*
D012337:RNA, Ribosomal, 18S; D012341:RNA, Ribosomal, 5S