title: Human syntaxin 3 is localized apically in human intestinal cells.
abstract: To understand the molecular mechanisms underlying sorting of apical and basolateral membrane components in human intestinal epithelial cells, we have cloned the human homolog of rat syntaxin 3 and looked for its subcellular localization. Endogenous human syntaxin 3 was found to be localized at the apical membrane of colon epithelial and Caco-2 cells. This apical localization was confirmed by confocal microscopy after transfection of the cDNA coding for either full length or N-terminally truncated human syntaxin 3 in Caco-2 cells. Furthermore the signal(s) and machinery targeting human syntaxin 3 to the apical membrane of epithelial cells are conserved between species since human syntaxin 3 was also localized at the apical membrane of canine MDCK cells and of epithelial cells in transgenic Drosophila melanogaster.
authors.affiliation: Laboratoire de Génétique et Physiologie du Développement, Campus de Luminy, Marsaille, France.
authors.forename: M H
authors.initials: MH
authors.lastname: Delgrossi
authors.forename: L
authors.initials: L
authors.lastname: Breuza
authors.forename: C
authors.initials: C
authors.lastname: Mirre
authors.forename: P
authors.initials: P
authors.lastname: Chavrier
authors.forename: A
authors.initials: A
authors.lastname: Le Bivic
mesh_terms: D000818:Animals; D001692:Biological Transport / Q000502:physiology; D019556:COS Cells / Q000737:chemistry; D018938:Caco-2 Cells / Q000737:chemistry*; D016764:Cell Polarity / Q000502:physiology*; D003001:Cloning, Molecular; D004331:Drosophila melanogaster; D004847:Epithelial Cells / Q000737:chemistry / Q000502:physiology; D015870:Gene Expression / Q000502:physiology; D006801:Humans; D007668:Kidney / Q000166:cytology; D008565:Membrane Proteins / Q000032:analysis* / Q000235:genetics / Q000378:metabolism; D009419:Nerve Tissue Proteins / Q000032:analysis / Q000235:genetics / Q000378:metabolism; D016133:Polymerase Chain Reaction; D050765:Qa-SNARE Proteins; D014162:Transfection
chemical_list: D008565:Membrane Proteins; D009419:Nerve Tissue Proteins; D050765:Qa-SNARE Proteins