title: Assessing enzyme substrate specificity using combinatorial libraries and electrospray ionization-Fourier transform ion cyclotron resonance mass spectrometry.
abstract: A model experiment for the 'on-line' screening of substrate libraries by enzymes using combinatorial libraries in combination with electrospray ionization-Fourier transform ion cyclotron resonance (ESI-FTICR) mass spectrometry has been performed. The reaction between the electrophilic substrate 1-chloro-2,4-dinitrobenzene and component of a H-gamma-Glu-Cys-Xxx-OH library, catalyzed by glutathione-S-transferase, has been monitored. It shows the feasibility of 'two-dimensional' screening of substrate libraries by ESI-FTICR mass spectrometry.
authors.affiliation: Department of Chemistry, University of Florida, Gainesville 32611-7200, USA.
authors.forename: M
authors.initials: M
authors.lastname: Wigger
authors.forename: J P
authors.initials: JP
authors.lastname: Nawrocki
authors.forename: C H
authors.initials: CH
authors.lastname: Watson
authors.forename: J R
authors.initials: JR
authors.lastname: Eyler
authors.forename: S A
authors.initials: SA
authors.lastname: Benner
mesh_terms: D017357:Cyclotrons; D004137:Dinitrochlorobenzene; D005583:Fourier Analysis; D005982:Glutathione Transferase / Q000378:metabolism; D007990:Libraries; D013058:Mass Spectrometry; D013379:Substrate Specificity
chemical_list: D004137:Dinitrochlorobenzene; D005982:Glutathione Transferase