title: Analysis of fumonisin B1 in rodent feed by CE with fluorescence detection of the FMOC derivative.
abstract: A fast, simple, and environmentally friendly method has been developed for the analysis of fumonisin B1 (FB1) in rodent feed using CE. FB1 is the major fumonisin metabolite produced by the fungus Fusarium moniliforme and has been implicated in human and animal diseases. FB1 was extracted from rodent feed with acetonitrile/water (50/50) (vol/vol) and cleaned up with a C18 Sep-Pak Vac cartridge (Waters Corp., Milford, MA, U.S.A.). FB1 was quantitated after elution from the column using capillary electrophoresis with a 25-mM sodium borate buffer (pH 9.0) containing 10% acetonitrile and fluorescence detection of the (9-fluorenylmethyl) chloroformate (FMOC) derivative. The minimum detectable amount in rodent feed was 0.5 pmm. Recovery values in spiked rodent feed averaged more than 87% over the 2-20 ppm range.
authors.affiliation: U.S. Food and Drug Administration, National Center for Toxicological Research, Jefferson, AR 72079-9502, USA.
authors.forename: M
authors.initials: M
authors.lastname: Holcomb
authors.forename: H C
authors.initials: HC
authors.lastname: Thompson
mesh_terms: D000821:Animal Feed / Q000032:analysis*; D000818:Animals; D002264:Carboxylic Acids / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D019075:Electrophoresis, Capillary / Q000379:methods; D005449:Fluorenes; D037341:Fumonisins; D006801:Humans; D007202:Indicators and Reagents; D009183:Mycotoxins / Q000032:analysis*; D012377:Rodentia; D012680:Sensitivity and Specificity; D013050:Spectrometry, Fluorescence
chemical_list: D002264:Carboxylic Acids; D005449:Fluorenes; D037341:Fumonisins; D007202:Indicators and Reagents; D009183:Mycotoxins; C056933:fumonisin B1