GAL4-responsive UAS-tau as a tool for studying the anatomy and development of the Drosophila central nervous system.
To improve the quality of cytoplasmic labelling of GAL4-expressing cells in Drosophila enhancer-trap and transgenic strains, a new GAL4-responsive reporter UAS-tau, which features a bovine tau cDNA under control of a yeast upstream activation sequence (UAS), was tested. Tau, a microtubule-associated protein, is distributed actively and evenly into all cellular processes. Monoclonal anti-bovine Tau antibody reveals the axonal structure of the labelled cells with detail similar to that of Golgi impregnation. We demonstrate that the UAS-tau system is especially useful for studying processes of differentiation and reorganisation of identified neurones during postembryonic development.
Ito
K
K
Institut für Genetik, Universität Mainz, Saarstr. 21, D-55122 Mainz, Germany. itokei@fly.erato.jst.go.jp
Sass
H
H
Urban
J
J
Hofbauer
A
A
Schneuwly
S
S
D000818:Animals; D030801:Animals, Genetically Modified; D000911:Antibodies, Monoclonal; D002417:Cattle; D002490:Central Nervous System / Q000033:anatomy & histology / Q000166:cytology / Q000254:growth & development; D004268:DNA-Binding Proteins / Q000235:genetics; D004330:Drosophila / Q000033:anatomy & histology / Q000166:cytology / Q000254:growth & development*; D004742:Enhancer Elements, Genetic / Q000502:physiology; D005656:Fungal Proteins / Q000235:genetics*; D018507:Gene Expression Regulation, Developmental / Q000502:physiology; D017930:Genes, Reporter; D005820:Genetic Testing / Q000379:methods; D008675:Metamorphosis, Biological / Q000502:physiology; D009474:Neurons / Q000502:physiology; D029701:Saccharomyces cerevisiae Proteins; D014157:Transcription Factors; D016875:tau Proteins / Q000235:genetics* / Q000276:immunology
D000911:Antibodies, Monoclonal; D004268:DNA-Binding Proteins; D005656:Fungal Proteins; C052130:GAL4 protein, S cerevisiae; D029701:Saccharomyces cerevisiae Proteins; D014157:Transcription Factors; D016875:tau Proteins