title: Pilin C-terminal peptide binds asialo-GM1 in liposomes: a 2H-NMR study.
abstract: Wideline 2H-NMR observations are described demonstrating the interaction of a synthetic peptide (PAK), representing residues 128-144 of the binding domain of pilin surface protein from Pseudomonas aeruginosa, with a complex glycosphingolipid thought to be its natural receptor. The receptor glycolipid (asialo-GM1) carried 2H probe nuclei on the terminal and next-to-terminal carbohydrate residues and was present as a minor component in fluid phosphatidylcholine liposomes. The peptide induced spectral changes that could be understood as arising from receptor motional changes, without receptor immobilization on the NMR time scale of 10(4) s-1. Spectral effects were reversed by reduction of the single peptide disulfide bond--a structural feature previously shown to be a determinant of PAK conformation (Campbell AP, McInnes C, Hodges RS, Sykes BD. 1995. Biochemistry 34:16255-16268). This is the first demonstration of PAK interaction with its epithelial cell receptor in liposomes.
authors.affiliation: Department of Biochemistry, University of Western Ontario, London, Canada.
authors.forename: D H
authors.initials: DH
authors.lastname: Jones
authors.forename: R S
authors.initials: RS
authors.lastname: Hodges
authors.forename: K R
authors.initials: KR
authors.lastname: Barber
authors.forename: C W
authors.initials: CW
authors.lastname: Grant
mesh_terms: D000595:Amino Acid Sequence; D001425:Bacterial Outer Membrane Proteins / Q000378:metabolism*; D003903:Deuterium; D036781:Fimbriae Proteins; D005677:G(M1) Ganglioside / Q000378:metabolism*; D008081:Liposomes; D008969:Molecular Sequence Data; D019906:Nuclear Magnetic Resonance, Biomolecular / Q000379:methods; D010446:Peptide Fragments / Q000378:metabolism; D011485:Protein Binding; D011956:Receptors, Cell Surface / Q000378:metabolism
chemical_list: D001425:Bacterial Outer Membrane Proteins; D008081:Liposomes; D010446:Peptide Fragments; D011956:Receptors, Cell Surface; D036781:Fimbriae Proteins; D005677:G(M1) Ganglioside; C018835:asialo GM1 ganglioside; D003903:Deuterium