In vitro and in vivo mutagenicity of the butadiene metabolites butadiene diolepoxide, butadiene monoepoxide and diepoxybutane.
Three metabolites of 1,3-butadiene, namely butadiene diolepoxide, butadiene monoepoxide and diepoxybutane, were tested in the bacterial mutation assay using Salmonella typhimurium strain TA100 with and without metabolic activation (S9 mix). All three compounds showed a mutagenic response. The bifunctional epoxide was more effective than the diolepoxide which was more effective than the monoepoxide. Toxicity appeared to follow the ranking of the chemicals for their mutagenic potency. The monoepoxide and the diolepoxide were also tested for induction of micronuclei in mouse bone marrow erythrocytes and for dominant lethal mutation induction in postmeiotic male mouse germ cells. The effects of the diepoxide in both in vivo tests have been published earlier. In the micronucleus assay, the three metabolites gave a positive response whereby the diepoxide was more effective than the monoepoxide which was more effective than the diolepoxide. In contrast to the diepoxide which was positive at a dose as low as 36 mg/kg, the monoepoxide and the diol did not show an induction of dominant lethal effects up to doses of 120 and 240 mg/kg, respectively. It is concluded that the metabolites were mutagenic in bacteria without metabolic activation and clastogenic in mouse bone marrow; only the bifunctional diepoxide, however, was active in postmeiotic male mouse germ cells.
Adler
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ID
GSF-Institut für Säugetiergenetik, Oberschleissheim, Germany.
Kliesch
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U
Nylund
L
L
Peltonen
K
K
D000818:Animals; D001854:Bone Marrow Cells / Q000187:drug effects* / Q000473:pathology; D002070:Butadienes; D003433:Crosses, Genetic; D004852:Epoxy Compounds / Q000494:pharmacology* / Q000633:toxicity*; D005260:Female; D005313:Fetal Death; D005804:Genes, Lethal; D006018:Glycols / Q000494:pharmacology* / Q000633:toxicity*; D008098:Litter Size / Q000187:drug effects*; D008297:Male; D051379:Mice; D008809:Mice, Inbred C3H; D008815:Mice, Inbred Strains; D015162:Micronucleus Tests; D009152:Mutagenicity Tests; D009153:Mutagens / Q000633:toxicity*; D011247:Pregnancy; D012486:Salmonella typhimurium / Q000187:drug effects; D013329:Structure-Activity Relationship
D002070:Butadienes; D004852:Epoxy Compounds; D006018:Glycols; D009153:Mutagens; C080053:3,4-epoxybutane-1,2-diol; C024370:3,4-epoxy-1-butene; C007366:diepoxybutane; C031763:1,3-butadiene