Xanthomonas campestris pv. campestris lpsI and lpsJ genes encoding putative proteins with sequence similarity to the alpha- and beta-subunits of 3-oxoacid CoA-transferases are involved in LPS biosynthesis.
A 2.1-kb SmaI-EcoRI DNA fragment upstream of the xanA and xanB genes of Xanthomonas campestris pv. campestris carries two ORFs encoding putative proteins with sequence similarities to the alpha- and beta-subunits of 3-oxoacid-CoA transferases. The two ORFs were termed lpsI and lpsJ because strains carrying appropriate mutations showed an autoagglutination phenotype and because lipopolysaccharides of these mutant strains were altered according to silver-stained polyacrylamide gels. The monosaccharide composition of the exopolysaccharide xanthan produced by lpsI and lpsJ mutants remained unchanged.
Steinmann
D
D
Lehrstuhl für Genetik, Universität Bielefeld, Germany.
Köplin
R
R
Pühler
A
A
Niehaus
K
K
D000595:Amino Acid Sequence; D015256:Coenzyme A-Transferases / Q000737:chemistry / Q000235:genetics* / Q000302:isolation & purification; D004252:DNA Mutational Analysis; D004269:DNA, Bacterial / Q000737:chemistry / Q000302:isolation & purification; D005798:Genes, Bacterial; D008070:Lipopolysaccharides / Q000096:biosynthesis*; D008359:Mannose-6-Phosphate Isomerase / Q000235:genetics; D008969:Molecular Sequence Data; D009154:Mutation; D009713:Nucleotidyltransferases / Q000235:genetics; D016366:Open Reading Frames; D010733:Phosphoglucomutase / Q000235:genetics; D017875:Phosphotransferases (Phosphomutases) / Q000235:genetics; D011135:Polysaccharides, Bacterial / Q000096:biosynthesis; D017386:Sequence Homology, Amino Acid; D016959:Xanthomonas campestris / Q000201:enzymology* / Q000235:genetics*
D004269:DNA, Bacterial; D008070:Lipopolysaccharides; D011135:Polysaccharides, Bacterial; D009713:Nucleotidyltransferases; C026917:mannose 1-phosphate guanylyltransferase; D015256:Coenzyme A-Transferases; C021460:3-ketoacid CoA-transferase; D008359:Mannose-6-Phosphate Isomerase; D017875:Phosphotransferases (Phosphomutases); D010733:Phosphoglucomutase; C052799:phosphomannomutase; C002563:xanthan gum