Assessment of dot-blot ELISA sensitivity on membrane sorbent using various peroxidase substrates.
The sensitivity of the peroxidase reaction in dot-blot ELISA significantly depends on the substrate. The highest sensitivity is observed using benzidine and diamine-phenol combinations as the substrates due to the reaction of the coupled oxidation (NADI).
Bogoyavlensky
A P
AP
Institute of Microbiology and Virology, Almaty, Kazakhstan.
Digel
I E
IE
Berezin
V E
VE
D000818:Animals; D002642:Chick Embryo; D004797:Enzyme-Linked Immunosorbent Assay / Q000592:standards*; D010084:Oxidation-Reduction; D010544:Peroxidases / Q000378:metabolism*; D012680:Sensitivity and Specificity; D013379:Substrate Specificity
D010544:Peroxidases