[Transfer of bacterial genes for proline synthesis in plants and their expression by various plant promotors].
Accumulation of free proline (Pro) by bacteria and plants serves as a mechanism protecting these organisms from abiotic stress. We introduced two E. coli genes, proBosm, encoding a mutant variant of the first enzyme of the Pro biosynthetic pathway, gamma-glutamyl kinase, and proA, encoding the second enzyme of the Pro pathway, glutamyl-gamma-semialdehyde dehydrogenase, into tobacco plants. The spontaneous proBosm mutation was selected for Pro overproduction and mapped to the N-terminus of a polypeptide chain; it was found to result from one amino-acid substitution. Two E. coli genes were simultaneously introduced into tobacco plants, each under the control of a strong constitutive CaMV 35S promoter, which contained a duplicated sequence of enhancer, or the root-specific Pmas promoter sequence. Transgenic plants were characterized by Pro overproduction, increased resistance to L-azetidine-2-carboxylic acid (a toxic analog of Pro), and tolerance to salt stress.
Sokhansandzh
A
A
Neumyvakin
L V
LV
Moseĭko
N A
NA
Piruzian
E S
ES
D016960:Agrobacterium tumefaciens / Q000235:genetics; D000445:Aldehyde Oxidoreductases / Q000235:genetics; D004926:Escherichia coli / Q000235:genetics; D018506:Gene Expression Regulation, Plant; D018014:Gene Transfer Techniques; D005798:Genes, Bacterial; D005822:Genetic Vectors; D050698:Glutamate-5-Semialdehyde Dehydrogenase; D017851:Phosphotransferases (Carboxyl Group Acceptor) / Q000235:genetics; D030821:Plants, Genetically Modified; D010947:Plants, Toxic; D010957:Plasmids; D011392:Proline / Q000096:biosynthesis*; D011401:Promoter Regions, Genetic; D014026:Nicotiana / Q000235:genetics* / Q000378:metabolism; D014170:Transformation, Genetic
D011392:Proline; D000445:Aldehyde Oxidoreductases; D050698:Glutamate-5-Semialdehyde Dehydrogenase; D017851:Phosphotransferases (Carboxyl Group Acceptor); C020650:glutamate 5-kinase