NF-M trans-activates the human DNA topoisomerase II alpha promoter independently of c-Myb in HL-60 cells.
Identifying transcriptional regulators of DNA topoisomerase II alpha (topo II alpha) is essential to decipher the mechanisms underlying leukemia cell resistance to topo II-directed antitumor drugs. We have previously reported that the proto-oncogene transcription factor c-Myb transactivates the topo II alpha promoter in several hematopoietic cell lines. Currently, we investigate whether NF-M, a C/EBP beta family member, cooperates with c-Myb in activating topo II alpha transcription. Although NF-M is the most efficacious trans-activator of topo II alpha that we have examined (approximately 38-fold over basal), NF-M does not appear to be involved in the endogenous transcriptional regulation of topo II alpha. Interestingly, we report that the sodium butyrate-dependent induction of the topo II alpha promoter observed previously appears to be mediated by c-Myb, independent of NF-M.
Brandt
T L
TL
Department of Pharmaceutical Sciences, University of Colorado School of Pharmacy, Denver, USA.
Kroll
D J
DJ
D000818:Animals; D000951:Antigens, Neoplasm; D022762:CCAAT-Enhancer-Binding Proteins; D019556:COS Cells; D004250:DNA Topoisomerases, Type II / Q000235:genetics*; D004268:DNA-Binding Proteins / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D018922:HL-60 Cells; D006367:HeLa Cells; D006801:Humans; D007527:Isoenzymes / Q000235:genetics*; D009687:Nuclear Proteins / Q000494:pharmacology*; D011401:Promoter Regions, Genetic; D000090063:Proto-Oncogene Mas; D014157:Transcription Factors; D015533:Transcriptional Activation
D000951:Antigens, Neoplasm; D022762:CCAAT-Enhancer-Binding Proteins; D004268:DNA-Binding Proteins; D007527:Isoenzymes; C000715470:MAS1 protein, human; D009687:Nuclear Proteins; D000090063:Proto-Oncogene Mas; D014157:Transcription Factors; D004250:DNA Topoisomerases, Type II