title: Cooking with calcium: the recipes for composing global signals from elementary events.
abstract: Recent studies have suggested that global intracellular Ca2+ signals arise from the summation and coordination of subcellular elementary release events (e.g., "Ca2+ puffs"), although the modes of recruitment of such signals are unknown. In order to understand how cells utilize elementary Ca2+ release events, we imaged Ca2+ transients evoked through the phosphoinositide pathway in HeLa cells using confocal microscopy. During the pacemaker phase leading to the global Ca2+ signal, elementary Ca2+ release events were recruited in (1) frequency, (2) amplitude, and (3) spatial domains. Since each digital elementary event contributes to a small change of the analog cytosolic Ca2+ concentration, the net effect of the advancement in the three domains is to drive the ambient Ca2+ concentration toward a threshold where the signal becomes regenerative, resulting in a global Ca2+ wave.
authors.affiliation: Laboratory of Molecular Signalling, The Babraham Institute, Cambridge, United Kingdom.
authors.forename: M D
authors.initials: MD
authors.lastname: Bootman
authors.forename: M J
authors.initials: MJ
authors.lastname: Berridge
authors.forename: P
authors.initials: P
authors.lastname: Lipp
mesh_terms: D002118:Calcium / Q000378:metabolism*; D006367:HeLa Cells; D006632:Histamine / Q000494:pharmacology; D006801:Humans; D018613:Microscopy, Confocal; D015398:Signal Transduction / Q000502:physiology*
chemical_list: D006632:Histamine; D002118:Calcium