title: Molecular evolution of the tet(M) gene in Gardnerella vaginalis.
abstract: Five clinical isolates of Gardnerella vaginalis known to carry the tetracycline resistance determinant Tet M were examined by hybridization and nucleotide sequencing. Four of the strains carried tet(M) genes with identical sequences. The two versions of the tet(M) gene found in G. vaginalis did not show complete identity with other published tet(M) sequences, but showed mosaic structures with regions of homology to tet(M) gene sequences from Tn916, Tn1545 and the American type plasmid found in Neisseria gonorrhoeae. Hybridization studies showed that all isolates carried the tet(M) gene on a single HindII restriction fragment of variable length. No evidence was found for the presence of sequences homologous to the transposition functions of Tn916.
authors.affiliation: Biotechnology Research Institute, Suzhou Medical College, People's Republic of China, UK.
authors.forename: R
authors.initials: R
authors.lastname: Huang
authors.forename: D M
authors.initials: DM
authors.lastname: Gascoyne-Binzi
authors.forename: P M
authors.initials: PM
authors.lastname: Hawkey
authors.forename: M
authors.initials: M
authors.lastname: Yu
authors.forename: J
authors.initials: J
authors.lastname: Heritage
authors.forename: A
authors.initials: A
authors.lastname: Eley
mesh_terms: D001483:Base Sequence; D004251:DNA Transposable Elements; D006194:Gardnerella vaginalis / Q000187:drug effects / Q000235:genetics*; D008969:Molecular Sequence Data; D011815:R Factors; D012097:Repressor Proteins / Q000235:genetics*; D013753:Tetracycline Resistance / Q000235:genetics*
chemical_list: D004251:DNA Transposable Elements; D012097:Repressor Proteins; C034811:tetracycline resistance-encoding transposon repressor protein