Effects of gamma-butyric acid on the release of thyrotropin-releasing hormone from the rat retina in vitro.
Effects of gamma-butyric acid (GABA) on the release of thyrotropin-releasing hormone (TRH) from the rat retina in vitro were studied. The rat retina was incubated in medium 199 (pH 7.4) with 1.0 mg/ml of bacitracin and 100 micrograms/ml of ascorbic acid (medium). The amount of TRH release into the medium was measured by radioimmunoassay. The TRH release from the rat retina was inhibited significantly in a dose-related manner with the addition of GABA, but not with bicuculline. The inhibitory effect of GABA on TRH release from the retina was blocked by adding bicuculline to the medium. The findings suggest that the GABAergic system inhibits TRH release from the rat retina in vitro.
Mitsuma
T
T
Fourth Department of Internal Medicine, Aichi Medical University, Japan.
Rhue
N
N
Kayama
M
M
Yokoi
Y
Y
Izumi
M
M
Adachi
K
K
Wago
T
T
Hirooka
Y
Y
Nogimori
T
T
D000818:Animals; D001414:Bacitracin / Q000494:pharmacology; D001640:Bicuculline / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D018756:GABA Antagonists / Q000494:pharmacology; D006863:Hydrogen-Ion Concentration; D008297:Male; D051381:Rats; D017208:Rats, Wistar; D012160:Retina / Q000187:drug effects* / Q000378:metabolism*; D013973:Thyrotropin-Releasing Hormone / Q000378:metabolism*; D005680:gamma-Aminobutyric Acid / Q000008:administration & dosage / Q000494:pharmacology*
D018756:GABA Antagonists; D001414:Bacitracin; D005680:gamma-Aminobutyric Acid; D013973:Thyrotropin-Releasing Hormone; D001640:Bicuculline