Designing recombinant Pseudomonas strains to enhance biodesulfurization.
The dsz biodesulfurization cluster from Rhodococcus erythropolis IGTS8 has been engineered under the control of heterologous broad-host-range regulatory signals to alleviate the mechanism of sulfur repression, and it was stably inserted into the chromosomes of different Pseudomonas strains. The recombinant bacteria were able to desulfurize dibenzothiophene more efficiently than the native host. Furthermore, these new biocatalysts combine relevant industrial and environmental traits, such as production of biosurfactants, with the enhanced biodesulfurization phenotype.
Gallardo
M E
ME
Departamento de Microbiología Molecular, Centro de Investigaciones Biológicas, Consejo Superior de Investigaciones Científicas, Madrid, Spain.
Ferrández
A
A
De Lorenzo
V
V
García
J L
JL
Díaz
E
E
D001673:Biodegradation, Environmental; D003001:Cloning, Molecular; D003574:Cytochrome c Group / Q000235:genetics; D004269:DNA, Bacterial / Q000032:analysis / Q000235:genetics; D015870:Gene Expression; D005798:Genes, Bacterial; D010088:Oxidoreductases / Q000235:genetics*; D010957:Plasmids; D011549:Pseudomonas / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism*; D011995:Recombination, Genetic; D012240:Rhodococcus / Q000235:genetics; D013876:Thiophenes / Q000378:metabolism
D003574:Cytochrome c Group; D004269:DNA, Bacterial; D013876:Thiophenes; D010088:Oxidoreductases; C090100:dibenzothiophene monooxygenase; C090099:diheme cytochrome c; C064500:flavocytochrome c sulfide dehydrogenase; C016366:dibenzothiophene