High frequency gene transfer by microprojectile bombardment of intact conidia from the entomopathogenic fungus Paecilomyces fumosoroseus.
Two different methods, (i) PEG and (ii) biolistic, were employed to transform protoplasts and conidia of Paecilomyces fumosoroseus using hygromycin resistance as selectable marker. Transformation frequencies varied from 1.9 to 2.5 transformants microgram-1 of DNA by the PEG method, and from 33 to 153 transformants microgram-1 of DNA by the biolistic procedure.
Barreto
C C
CC
Departamento de Biologia Celular, Universidade de Brasilia, Brazil.
Alves
L C
LC
Aragão
F J
FJ
Rech
E
E
Schrank
A
A
Vainstein
M H
MH
D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology; D004271:DNA, Fungal / Q000008:administration & dosage / Q000235:genetics; D004352:Drug Resistance, Microbial / Q000235:genetics; D005069:Evaluation Studies as Topic; D018014:Gene Transfer Techniques; D005819:Genetic Markers; D006921:Hygromycin B / Q000494:pharmacology; D007313:Insecta / Q000382:microbiology; D010143:Paecilomyces / Q000187:drug effects / Q000235:genetics* / Q000472:pathogenicity; D010572:Pest Control, Biological; D011092:Polyethylene Glycols; D011523:Protoplasts / Q000187:drug effects; D014170:Transformation, Genetic
D000900:Anti-Bacterial Agents; D004271:DNA, Fungal; D005819:Genetic Markers; D011092:Polyethylene Glycols; D006921:Hygromycin B