T cell activation in vivo by dengue virus infection.
It is accepted that T cells play a critical role during virus infections; however, T cell responses in vivo in acute stage of virus infection are not understood. We examined T cell activation in vivo in two volunteers who developed dengue fever in response to vaccination with a candidate live dengue vaccine. Serial plasma collected from the volunteers from day 0 (before infection) to day 17 after infection were examined for levels of soluble interleukin-2 receptor (sIL-2R), soluble CD4 (sCD4), soluble CD8 (sCD8), interleukin-2 (IL-2) and interferon gamma (INF gamma). Elevation of the levels of sIL-2R, IFN gamma, sCD4 and IL-2 became obvious during the period of viremia and was followed by a later increase in the level of sCD8. The levels of IFN gamma and sIL-2R declined after the end of the period of viremia. These results indicate that i. T cells are activated in vivo by dengue virus infection ii. activation of CD4+ T cells occurs during the period of viremia iii. activation of CD8+ T cells follows CD4+ T cell activation. These results suggest that activation of T cells in vivo may contribute to controlling acute dengue virus infections.
Kurane
I
I
Department of Medicine, University of Massachusetts Medical Center, Worcester 01655, USA.
Innis
B L
BL
Hoke
C H
CH
Eckels
K H
KH
Meager
A
A
Janus
J
J
Ennis
F A
FA
D000328:Adult; D015704:CD4 Antigens / Q000097:blood; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology; D016827:CD8 Antigens / Q000097:blood; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology; D003715:Dengue / Q000209:etiology / Q000276:immunology*; D006801:Humans; D007371:Interferon-gamma / Q000097:blood; D007376:Interleukin-2 / Q000097:blood; D008213:Lymphocyte Activation; D008297:Male; D015375:Receptors, Interleukin-2 / Q000097:blood; D012995:Solubility; D013601:T-Lymphocytes / Q000276:immunology*; D014613:Vaccines, Attenuated / Q000009:adverse effects; D014765:Viral Vaccines / Q000009:adverse effects; D014766:Viremia / Q000276:immunology
D015704:CD4 Antigens; D016827:CD8 Antigens; D007376:Interleukin-2; D015375:Receptors, Interleukin-2; D014613:Vaccines, Attenuated; D014765:Viral Vaccines; D007371:Interferon-gamma