title: Ovine squamous cell carcinoma: immunocharacterisation of neoplastic cells and peritumoural cellular infiltrate.
abstract: An immunohistochemical characterisation of neoplastic cells and peritumoural cellular infiltrate of ovine squamous cell carcinomas (OSCC) in different stages of growth was carried out using a commercially-available panel of seven antibodies, and formalin-fixed and paraffin-embedded tissue samples. Neoplastic cells reacted with the anti-keratin monoclonal antibody RCK-102 and with an anti-keratin polyclonal antibody, whereas they were unreactive with the anti-keratin monoclonal antibody NCL-5D3. The tumour cells were unreactive with the anti-vimentin polyclonal antibody. Increased numbers of CD3+ T-lymphocytes, IgG-containing plasma cells and lysozyme+ macrophages were associated with precancerous lesions (actinic keratosis) and with early OSCC. These results show that early lesions of OSCC induce an in situ cellular and humoral immune response. In advanced OSCC, the number of CD3+ T-lymphocytes and macrophages in the peri- and intratumoural cellular infiltrate remained high, whereas a significant decrease of IgG-containing plasma cells was found compared with early OSCC. This finding suggests an in situ decrease in the humoral immune response in advanced OSCC.
authors.affiliation: Department of Comparative Pathology, Faculty of Veterinary Medicine, University of Córdoba, Spain.
authors.forename: J
authors.initials: J
authors.lastname: Pérez
authors.forename: A
authors.initials: A
authors.lastname: Méndez
authors.forename: F C
authors.initials: FC
authors.lastname: de Lara
authors.forename: M P
authors.initials: MP
authors.lastname: Martín
authors.forename: E
authors.initials: E
authors.lastname: Mozos
mesh_terms: D000818:Animals; D017252:CD3 Complex / Q000032:analysis; D002294:Carcinoma, Squamous Cell / Q000737:chemistry / Q000473:pathology / Q000662:veterinary*; D002417:Cattle; D004428:Ear Neoplasms / Q000737:chemistry / Q000473:pathology / Q000662:veterinary*; D005142:Eyelid Neoplasms / Q000737:chemistry / Q000473:pathology / Q000662:veterinary*; D006801:Humans; D007106:Immune Sera / Q000032:analysis / Q000276:immunology; D007074:Immunoglobulin G / Q000032:analysis / Q000276:immunology; D007150:Immunohistochemistry / Q000379:methods; D007633:Keratins / Q000032:analysis / Q000276:immunology; D008264:Macrophages / Q000737:chemistry / Q000473:pathology; D009113:Muramidase / Q000032:analysis / Q000276:immunology; D012756:Sheep; D012757:Sheep Diseases / Q000378:metabolism / Q000473:pathology*; D012878:Skin Neoplasms / Q000737:chemistry / Q000473:pathology / Q000662:veterinary*; D013601:T-Lymphocytes / Q000737:chemistry / Q000473:pathology; D014746:Vimentin / Q000032:analysis / Q000276:immunology
chemical_list: D017252:CD3 Complex; D007106:Immune Sera; D007074:Immunoglobulin G; D014746:Vimentin; D007633:Keratins; D009113:Muramidase