Synthesis of cytokinin glucuronides for the selection of transgenic plant cells.
Glucuronide derivatives of cytokinins have been synthesized for use as agents for the selection of plant cells transformed with a beta-glucuronidase (GUS) gene. In this selection system, the GUS gene functions as both a selectable, as well as a screenable gene. GUS liberates active cytokinin from inactive cytokinin glucuronides which then stimulates growth and regeneration of the transformed cells. The frequently used cytokinin N6-benzyladenine was conjugated to glucuronic acid at N-3 or at N-9 but only the former was a substrate for GUS. The glucuronide of isopentenyladenine was also made, by coupling at the N-3. This compound was readily hydrolysed by GUS.
Okkels
F T
FT
Danisco Biotechnology, Copenhagen, Denmark.
Ward
J L
JL
Joersbo
M
M
D002455:Cell Division / Q000187:drug effects; D003583:Cytokinins / Q000096:biosynthesis / Q000494:pharmacology; D004926:Escherichia coli / Q000201:enzymology; D017930:Genes, Reporter; D005965:Glucuronates / Q000138:chemical synthesis* / Q000737:chemistry / Q000378:metabolism*; D005966:Glucuronidase / Q000096:biosynthesis; D019906:Nuclear Magnetic Resonance, Biomolecular; D030821:Plants, Genetically Modified; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis
D003583:Cytokinins; D005965:Glucuronates; D011993:Recombinant Fusion Proteins; D005966:Glucuronidase