title: Development of the immune system in severe combined immunodeficiency mice reconstituted with transferred fetal liver cells.
abstract: Severe combined immunodeficiency (SCID) mice were immunologically reconstituted by the transfer of fetal liver cells (FLC) from BALB/c (SCID-isoFLC mice) or C57BL/6 mice (SCID-alloFLC mice). The developmental process of the immune system in the peripheral blood (PB) was almost comparable between SCID-isoFLC and SCID-isoFLC mice. Analysis of the lymphoid organs and the PB from SCID-alloFLC mice indicated that slg+ B cells appeared and were distributed to the periphery within 2 weeks after the transfer of FLC. It was also suggested that precursor T cells entered the thymus before 4 weeks after the transfer, and were differentiated into mature CD4+ or CD8+ T cells which then migrated to the periphery by 6 weeks. All of mature lymphocytes in the periphery of the SCID-alloFLC mice were shown to express donor-type H-2 antigens. Additionally, in the SCID-isoFLC mice, cell-mediated immunity such as rejection against alloantigens was functioning from 6 weeks, and humoral immune function was suggested by the detection of cells generating antigen-specific antibodies. We discuss that development of the immune system in SCID mice receiving transferred FLC was comparable to that normally seen in the fetal and neonatal stages.
authors.affiliation: Department of Pathology, Faculty of Medicine, Tokyo Medical and Dental University, Japan. a.wakabayashi.pth1@med.tmd.ac.jp
authors.forename: A
authors.initials: A
authors.lastname: Wakabayashi
authors.forename: Y
authors.initials: Y
authors.lastname: Eishi
authors.forename: K
authors.initials: K
authors.lastname: Nakamura
mesh_terms: D000818:Animals; D000831:Animals, Newborn / Q000276:immunology; D000917:Antibody Formation / Q000276:immunology; D000918:Antibody Specificity; D001402:B-Lymphocytes / Q000166:cytology / Q000276:immunology; D001769:Blood; D015496:CD4-Positive T-Lymphocytes / Q000166:cytology / Q000276:immunology; D018414:CD8-Positive T-Lymphocytes / Q000166:cytology / Q000276:immunology; D002454:Cell Differentiation; D002465:Cell Movement; D016332:Fetal Tissue Transplantation; D006084:Graft Rejection / Q000276:immunology; D006183:H-2 Antigens / Q000032:analysis; D018380:Hematopoietic Stem Cell Transplantation; D007107:Immune System / Q000276:immunology*; D007111:Immunity, Cellular / Q000276:immunology; D007519:Isoantigens / Q000276:immunology; D008099:Liver / Q000166:cytology*; D008214:Lymphocytes / Q000166:cytology / Q000276:immunology; D008221:Lymphoid Tissue / Q000276:immunology; D051379:Mice; D008807:Mice, Inbred BALB C; D008809:Mice, Inbred C3H; D008810:Mice, Inbred C57BL; D008815:Mice, Inbred Strains; D008819:Mice, Nude; D016513:Mice, SCID; D016511:Severe Combined Immunodeficiency / Q000276:immunology / Q000628:therapy*; D016038:Skin Transplantation / Q000276:immunology; D013601:T-Lymphocytes / Q000166:cytology / Q000276:immunology; D013950:Thymus Gland / Q000166:cytology / Q000276:immunology
chemical_list: D006183:H-2 Antigens; D007519:Isoantigens