[The effect of immunoregulation of preparation of Mycobacterium vaccea by nitrogen oxide].
OBJECTIVE
Preparation of M. vaccea on synthesis of nitrite [NO2-, an oxidative end product of nitric oxide (NO) synthesis] of peritoneal macrophages by different condition of animals.


METHODS
First, the BALB/c mice were immunized by preparation of M. vaccea and effect of synthesis of NO2- by murine peritoneal macrophage was detected. Second, guinea pigs intraperitonealy challenged with M. tuberculosis H37Rv were immunized by preparation of M. vaccea.


RESULTS
It was demonstrated that the effect of synthesis of NO2- of the murine peritoneal macrophage of the immunized mice was significantly higher than the unimmunized mice (P < 0.01) and synthesis of NO2- of peritoneal macrophage of challenged guinea pigs immunized with M. vaccea was significantly lower than that of challenged guinea pigs unimmunized with M. vaccea (P < 0.05).


CONCLUSIONS
The preparation of M. vaccea can not only increase the effect of releasing of NO2- of the peritoneal macrophage, but also regulate the effect of immuno-reaction of the animals so as to reduce the damage of the tissue.
Wang
G
G
National Institute for the Control of Pharmaceutical and Biological Products, Beijing.
Zhao
G
G
Li
X
X
D000276:Adjuvants, Immunologic; D000818:Animals; D005260:Female; D006168:Guinea Pigs; D017737:Macrophages, Peritoneal / Q000378:metabolism*; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D009161:Mycobacterium / Q000276:immunology*; D009569:Nitric Oxide / Q000096:biosynthesis*; D014376:Tuberculosis / Q000276:immunology* / Q000378:metabolism
D000276:Adjuvants, Immunologic; D009569:Nitric Oxide