title: Sequence analysis and characterization of phi O1205, a temperate bacteriophage infecting Streptococcus thermophilus CNRZ1205.
abstract: The complete nucleotide sequence of phi O1205, a temperate bacteriophage infecting Streptococcus thermophilus strain CNRZ1205, was determined. The phage genome has a unit length of 43,075 bp and appears to be packaged by the so-called headful mechanism. The genomic organization and structure of phi O1205 resemble those of several temperate lactococcal phages that display a life-cycle-specific organization, where ORFs believed to be involved in the lysogenic life-cycle are clustered and arranged in an orientation opposite to the ORFs supposedly involved in the lytic life-cycle. Database searches revealed putative functions for several identified ORFs and further indicated that phi O1205 is genetically related to a particular group of lactococcal phages. Three genes encoding the major structural proteins were identified on the phi O1205 genome. The phage attachment site attP, the bacterial attachment site attB, and the two phage/chromosome junctions attL and attR were identified and found to contain a 40 bp common core sequence.
authors.affiliation: National Food Biotechnology Centre, University College,Cork,Ireland.|Department of Microbiology, University College,Cork,Ireland.
authors.forename: Elizabeth
authors.initials: E
authors.lastname: Stanley
authors.affiliation: National Food Biotechnology Centre, University College,Cork,Ireland.|Department of Microbiology, University College,Cork,Ireland.
authors.forename: Gerald F
authors.initials: GF
authors.lastname: Fitzgerald
authors.affiliation: Department of Microbiology, University College,Cork,Ireland.
authors.forename: Claire Le
authors.initials: CL
authors.lastname: Marrec
authors.affiliation: National Food Biotechnology Centre, University College,Cork,Ireland.
authors.forename: Blandine
authors.initials: B
authors.lastname: Fayard
authors.affiliation: National Food Biotechnology Centre, University College,Cork,Ireland.|Department of Microbiology, University College,Cork,Ireland.
authors.forename: Douwe
authors.initials: D
authors.lastname: van Sinderen
mesh_terms: D000595:Amino Acid Sequence; D001287:Attachment Sites, Microbiological; D001483:Base Sequence; D004279:DNA, Viral / Q000235:genetics; D016679:Genome, Viral; D008969:Molecular Sequence Data; D008970:Molecular Weight; D016366:Open Reading Frames / Q000235:genetics*; D017421:Sequence Analysis; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D013291:Streptococcus / Q000821:virology; D017108:Streptococcus Phages / Q000235:genetics* / Q000648:ultrastructure; D015678:Viral Structural Proteins / Q000737:chemistry / Q000235:genetics; D019065:Virus Assembly
chemical_list: D004279:DNA, Viral; D015678:Viral Structural Proteins