Whole-cell and periplasmic protein banding patterns of environmental and human Bacteroides fragilis strains.
In order to investigate the relationship among virulent and avirulent Bacteroides fragilis strains, SDS-PAGE of whole-cell proteins (WP) and periplasmic proteins (PP) were used to establish a protein profile of strains isolated from human infections, fecal flora and environmental water. Despite different sources of the strains, no significant differences were observed as determined by the WP SDS-PAGE analysis. In contrast, the proteins obtained from the bacterial periplasm showed differences in the electrophoretic protein profile. Two distinct PP profile patterns were obtained. Pattern A included 6 out of the 8 virulent strains and pattern B, 6 out of 8 avirulent strains. Interestingly, an environmental strain that was capable of inducing abscesses in mice, had a PP profile highly similar to that of the virulent strains from human infections. These data indicate that PP from B. fragilis may be useful to characterize differences among virulent and avirulent strains. Moreover, strains isolated from environmental water may also be a source of exogenous infections by B. fragilis.
Domingues
R M
RM
Instituto de Microbiologia, Universidade Federal do Rio de Janeiro, Brasil.
Avelar
K E
KE
Souza
W G
WG
Moraes
S R
SR
Antunes
E N
EN
Oliveira
I A
IA
Ferreira
M C
MC
D000818:Animals; D001426:Bacterial Proteins / Q000032:analysis* / Q000302:isolation & purification; D001442:Bacteroides Infections / Q000382:microbiology; D001441:Bacteroides fragilis / Q000737:chemistry* / Q000145:classification / Q000472:pathogenicity; D004591:Electrophoresis, Polyacrylamide Gel; D005243:Feces / Q000382:microbiology; D006801:Humans; D051379:Mice; D019897:Periplasm / Q000737:chemistry; D014774:Virulence; D014871:Water Microbiology
D001426:Bacterial Proteins