[Expression of retroviral transduced human beta-globin gene in mouse fetal liver cells].
A replication-defective retrovirus vector containing the human beta-globin gene and 36-base-pair HS2 enhancer (N2A beta E36) was constructed and transferred into psi-2 and PA317 packaging cell lines. Titers of amphotropic recombinant retrovirus ranged from 10(3) to 10(4) CFU/ml. Southern blot analysis indicated that intact human beta-globin gene was stably integrated into the genome of the target cells. Northern blot hybridization analysis showed that human beta-globin gene could be expressed in mouse fetal liver cells.
Wang
H
H
Institute of Basic Medical Sciences, CAMS, Beijing.
Zhang
J
J
Xu
Y
Y
Wei
Q
Q
Liu
S
S
D000818:Animals; D002478:Cells, Cultured; D004274:DNA, Recombinant; D004742:Enhancer Elements, Genetic / Q000235:genetics*; D005333:Fetus; D018014:Gene Transfer Techniques; D005822:Genetic Vectors; D005914:Globins / Q000235:genetics*; D006801:Humans; D008099:Liver / Q000166:cytology*; D051379:Mice; D012190:Retroviridae / Q000235:genetics*; D014162:Transfection
D004274:DNA, Recombinant; D005914:Globins