[Effect of IGF-II on progesterone production by cultured rat luteal cells in vitro].
It has been reported recently that IGF-II plays an important role in the regulation of ovarian function. The aim of this study is to investigate whether IGF-II can affect progesterone production by cultured rat luteal cells. The results showed that IGF-II could significantly stimulate basal progesterone production in a dose-dependent manner without altering the cAMP content. IGF-II could enhance 3H-leu incorporation into protein and 3H-TdR incorporation into DNA of the luteal cells, but these effects could be inhibited by CYX and Act D. The above results suggest that stimulating effect of IGF-II on progesterone production by rat luteal cells may be related to enhanced protein and DNA synthesis.
Ni
J
J
Department of Physiology, Harbin Medical University.
Zhu
H
H
Zhang
W
W
Wang
J H
JH
Ma
Q N
QN
D000818:Animals; D002478:Cells, Cultured; D004247:DNA / Q000096:biosynthesis; D005260:Female; D007335:Insulin-Like Growth Factor II / Q000494:pharmacology*; D008184:Luteal Cells / Q000166:cytology / Q000378:metabolism*; D011374:Progesterone / Q000096:biosynthesis*; D051381:Rats; D017208:Rats, Wistar
D011374:Progesterone; D007335:Insulin-Like Growth Factor II; D004247:DNA