High-performance liquid chromatographic assay of arachidonic acid metabolites from cultured rat glial cells.
Although various methods can be used for detection of arachidonic acid (AA) metabolites, only few of them allow rapid and precise analysis of both cyclooxygenase and lipoxygenase metabolites in a single assay. We have developed a simple and rapid method to evaluate overall profiles of AA metabolites produced by primary cultured glial cells from newborn rats by using reversed-phase, high-performance liquid chromatography (RP-HPLC) and on-line radioisotope detection.
Matsuo
M
M
Department of Pediatrics, Saga Medical School, Japan.
Hamasaki
Y
Y
Miyazaki
S
S
D000818:Animals; D000831:Animals, Newborn; D016718:Arachidonic Acid / Q000378:metabolism*; D002478:Cells, Cultured; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D009457:Neuroglia / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar
D016718:Arachidonic Acid