[Rapid and simple detection of Ki-ras gene mutations in pancreatic adenocarcinoma by PCR-SSP].
In order to judge whether Ki-ras gene mutations are present and their mutation styles, three kinds of special sequence primers (SSP) for polymerase chain reaction with regard to the mutant styles (CGT, GTT and GAT) at codon 12 were used to study the mutations in frozen tissues of pancreatic adenocarcinoma. The amplification products were studied with conventional electrophoresis using acrylamide gels and stained with ethidium bromide to detect the mutations.


RESULTS
In 23 of 25 frozen pancreatic adenocarcinoma specimens, Ki-ras genes with mutations at codon 12 were found; 2 mutant styles were found in 8 of the 23 samples; no mutations were found in 11 normal pancreatic tissues, 12 benign pancreatic disease tissues and 3 bile duct carcinoma tissues. This method is rapid, convenient, specific as well as sensitive. We conclude that this may become a valuable diagnostic modality for pancreatic carcinoma and for differentiating benign pancreatic diseases from carcinoma.
Dai
C
C
Department of General Surgery, First Affiliated Hospital of Nanjing Medical University.
Liu
X
X
Du
J
J
D000230:Adenocarcinoma / Q000235:genetics*; D011905:Genes, ras; D006801:Humans; D010190:Pancreatic Neoplasms / Q000235:genetics*; D017354:Point Mutation; D016133:Polymerase Chain Reaction; D018807:Polymorphism, Single-Stranded Conformational