A banded topography in the developing rat's olfactory epithelial surface.
In situ hybridization studies from various laboratories have shown that the rat's olfactory epithelium has four distinct regions in which most putative odor receptors are located. To determine whether morphological features accompany this biochemical patterning, olfactory epithelial surfaces of rat nasal endoturbinates and septa were examined with scanning electron microscopy, placing particular emphasis on endoturbinate IIb. There was some morphological patterning at embryonic day 15 (E15), but distinct regions were not yet discernible. Regionalization became distinct at E16 and E18. Posterior regions (Regions 1 and 2) had much higher receptor cell knob densities than more anterior regions closer to the respiratory epithelium (Regions 3 and 4). Supporting cell microvilli were longer in Region 1 than in Region 2. Apices of cells surrounding the receptor cells were flatter in Regions 1 and 2 than in Regions 3 and, especially, Region 4. In Regions 1-3, these surrounding cells were made up mainly of supporting cells; in Region 4 they included respiratory cells. Regions 3 and 4 also had glandular openings and scattered microvillous cells that resemble hair cells of the ear. Older fetuses and adults showed similar evidence of patterning, but detailed examination was precluded by the increased length and entanglement of receptor cell cilia and supporting cell microvilli. In conclusion, a distinct topographic pattern, involving both receptor and surrounding cells, emerges during development of the rat olfactory epithelial surface. Location of the bands roughly matches the zones seen by in situ hybridization.
Menco
B P
BP
Department of Neurobiology and Physiology, Northwestern University, Evanston, Illinois 60208-3520, USA. bmenco@casbah.acns.nwu.edu
Jackson
J E
JE
D000818:Animals; D002923:Cilia / Q000648:ultrastructure; D005314:Embryonic and Fetal Development; D004848:Epithelium / Q000196:embryology / Q000648:ultrastructure; D005260:Female; D005865:Gestational Age; D008855:Microscopy, Electron, Scanning; D008871:Microvilli / Q000648:ultrastructure; D009831:Olfactory Mucosa / Q000166:cytology / Q000196:embryology*; D018034:Olfactory Receptor Neurons / Q000196:embryology / Q000648:ultrastructure; D051381:Rats; D014420:Turbinates / Q000196:embryology / Q000648:ultrastructure