Gaseous nitrogen dioxide increases the endogenous synthesis of carcinogenic N-nitrosodimethylamine in animals.
Inhalation of nitrogen dioxide (NO2) by mice administered orally amidopyrine (AP) and sodium nitrite resulted in increased biosynthesis of N-nitrosodimethylamine (NDMA), as determined by analysis using gas chromatography with thermal energy analyzer detector. These results were also confirmed indirectly in chronic experiments on rats using the system of biomarkers of NDMA formation (single-stranded DNA liver damages, alanine-aminotransferase, glutathione-S-transferase, and liver S9 fraction activity). The inhibition of NDMA metabolism by 4-methylpyrazol (4-MP) administration increases the sensitivity of NDMA biosynthesis assay in frozen whole-mouse powder. The results confirm that NO2 can serve as the precursor of nitrosamines.
Rubenchik
B L
BL
R. E. Kavetsky Institute of Experimental Pathology, Oncology, and Radiobiology, National Academy of Sciences of the Ukraine, Kiev, Ukraine.
Glavin
A A
AA
Galenko
P M
PM
Kilkichko
A A
AA
Oleinick
I O
IO
Artemov
K V
KV
D000280:Administration, Inhalation; D000284:Administration, Oral; D000410:Alanine Transaminase / Q000378:metabolism; D000632:Aminopyrine / Q000008:administration & dosage / Q000378:metabolism; D000818:Animals; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000008:administration & dosage / Q000378:metabolism; D015415:Biomarkers; D002273:Carcinogens / Q000378:metabolism*; D004249:DNA Damage / Q000187:drug effects; D004128:Dimethylnitrosamine / Q000378:metabolism*; D004338:Drug Combinations; D005982:Glutathione Transferase / Q000378:metabolism; D008099:Liver / Q000378:metabolism*; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D009153:Mutagens / Q000008:administration & dosage / Q000378:metabolism; D009585:Nitrogen Dioxide / Q000008:administration & dosage*; D051381:Rats; D012977:Sodium Nitrite / Q000008:administration & dosage / Q000378:metabolism
D000894:Anti-Inflammatory Agents, Non-Steroidal; D015415:Biomarkers; D002273:Carcinogens; D004338:Drug Combinations; D009153:Mutagens; D000632:Aminopyrine; D005982:Glutathione Transferase; D000410:Alanine Transaminase; D012977:Sodium Nitrite; D004128:Dimethylnitrosamine; D009585:Nitrogen Dioxide