title: New aspects of Leydig cell function.
abstract: Previous studies indicated that the Leydig cells of the human testes show similarities to neuroendocrine cells. In this context, the local synthesis of two neuroactive signaling molecules, namely nitric oxide (NO) and C-type natriuretic peptide (CNP), both acting via the second messenger, cyclic guanosine monophosphate (cGMP), might be of physiological relevance. By immunoblotting, immunohistochemical analyses and affinity crosslinking experiments, respectively, the presence of soluble guanylate cyclase (sGC), the NO receptor, and of guanylate cyclase B (GC-B), representing the CNP receptor, was demonstrated in Leydig cells, seminiferous tubules and blood vessels of the human testis. Moreover, cGMP and its binding protein cGMP-dependent protein kinase type I (GK I) were found in these structures. The functional activity of the two receptors was proved by generation of cGMP in response to treatments with the NO donor, sodium nitroprusside (SNP), and with CNP, respectively. As indicated by immunohistochemical analyses and by treatments of cells with either SNP or CNP, human Leydig tumour cells and MA10 cells, representing a mouse Leydig tumour cell line, were found to be distinguished by a reduced expression of the receptors for NO and CNP. Furthermore, expression levels of the components of the two cGMP-generating systems were found to be widely unchanged in Leydig cells during different ontogenetic stages. Though cGMP has been shown to influence testosterone release, the constant developmental expression patterns of NO and CNP apparently independent of differences in androgen production, the down-regulation of their receptors in tumorous cells, and the presence of GK I, may point to additional autocrine functions of these factors and of cGMP in Leydig cells. Moreover, possible paracrine actions of NO and CNP may include relaxation of seminiferous tubules and blood vessels in order to modulate sperm transport and testicular blood flow, respectively. These findings suggest that Leydig cell-derived factors may exert activities different from or in addition to those involved in the regulation of testosterone production.
authors.affiliation: Institute of Anatomy, University of Hamburg (UKE), Germany.
authors.forename: R
authors.initials: R
authors.lastname: Middendorff
authors.forename: D
authors.initials: D
authors.lastname: Müller
authors.forename: H J
authors.initials: HJ
authors.lastname: Paust
authors.forename: A F
authors.initials: AF
authors.lastname: Holstein
authors.forename: M S
authors.initials: MS
authors.lastname: Davidoff
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000818:Animals; D006152:Cyclic GMP / Q000378:metabolism; D017869:Cyclic GMP-Dependent Protein Kinases / Q000378:metabolism; D006162:Guanylate Cyclase / Q000235:genetics / Q000378:metabolism*; D006801:Humans; D007985:Leydig Cells / Q000378:metabolism / Q000502:physiology*; D008297:Male; D051379:Mice; D008875:Middle Aged; D020098:Natriuretic Peptide, C-Type; D019001:Nitric Oxide Synthase / Q000378:metabolism; D011506:Proteins / Q000235:genetics / Q000378:metabolism; D011817:Rabbits; D017461:Receptors, Atrial Natriuretic Factor / Q000235:genetics / Q000378:metabolism*; D017981:Receptors, Neurotransmitter / Q000378:metabolism*; D012671:Seminiferous Tubules / Q000378:metabolism
chemical_list: D011506:Proteins; D017981:Receptors, Neurotransmitter; D020098:Natriuretic Peptide, C-Type; D019001:Nitric Oxide Synthase; D017869:Cyclic GMP-Dependent Protein Kinases; D006162:Guanylate Cyclase; D017461:Receptors, Atrial Natriuretic Factor; C073616:atrial natriuretic factor receptor C; D006152:Cyclic GMP