Temporal brain imaging by a rapid scan ESR-CT system in rats receiving intraperitoneal injection of a methyl ester nitroxide radical.
We performed in vivo ESR-CT (electron spin resonance-computed tomography) on rats' heads, in which the blood-brain barrier-permeable nitroxide radical, 3-methoxycarbonyl-2,2,5,5-tetramethylpyrrolidine-1-oxyl (PCAM) was injected intraperitoneally, using a rapid scan ESR-CT system operating at 700 MHz. In a spatiotemporal study we found that different regions of the brain showed differences in the rate of decay of the radical. Repeated injection of PCAM gave clear ESR-CT images of the brain. We think that the present method is useful for evaluating the capacity to eliminate exogenous free radicals in some parts of the brain.
Yokoyama
H
H
Institute for Life Support Technology, Yamagata Technopolis Foundation, Matsuei, Japan. yokohide@cc.fmu.ac.jp
Itoh
O
O
Ogata
T
T
Obara
H
H
Ohya-Nishiguchi
H
H
Kamada
H
H
D000818:Animals; D001921:Brain / Q000033:anatomy & histology* / Q000000981:diagnostic imaging; D003497:Cyclic N-Oxides / Q000378:metabolism; D004578:Electron Spin Resonance Spectroscopy; D006257:Head / Q000033:anatomy & histology* / Q000000981:diagnostic imaging; D007274:Injections, Intraperitoneal; D008297:Male; D011759:Pyrrolidines / Q000378:metabolism; D011837:Radiation-Protective Agents / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D013113:Spin Labels; D014057:Tomography, X-Ray Computed
D003497:Cyclic N-Oxides; D011759:Pyrrolidines; D011837:Radiation-Protective Agents; D013113:Spin Labels; C077426:3-carbamoyl-2,2,5,5-tetramethyl-1-pyrrolidinyl-N-oxyl