A rapid method for chromatin structure analysis in the filamentous fungus Aspergillus nidulans.
A rapid method for nuclease digestion of Aspergillus nidulans chromatin is described. It overcomes the need for nuclear purification or protoplast preparation. The method is valid for the analysis of the nucleosomal repeat length in bulk chromatin, and allows the analysis of nucleosome phasing at a specific locus.
Gonzalez
R
R
Institut de Génétique et Microbiologie, URA CNRS D2225, Bâtiment 409, Université Paris-Sud, 91405 Orsay Cedex, France. rgonzalez@igmors.u-psud.fr
Scazzocchio
C
C
D001233:Aspergillus nidulans / Q000737:chemistry* / Q000235:genetics; D002843:Chromatin / Q000737:chemistry* / Q000235:genetics; D003850:Deoxyribonuclease I; D005800:Genes, Fungal; D008722:Methods; D008836:Micrococcal Nuclease; D015394:Molecular Structure; D009707:Nucleosomes / Q000737:chemistry / Q000235:genetics
D002843:Chromatin; D009707:Nucleosomes; D003850:Deoxyribonuclease I; D008836:Micrococcal Nuclease