Differentiation of cultured neuroblastoma induced by staurosporine and cyclic AMP: methods for assessing a neuronal phenotype.
In cultured neuronal cells, neurite outgrowth provides a prominent morphological marker for assessing neurotrophic activity derived from different sources. Permanent cell lines are often used for such studies; however, the process of differentiation should be evaluated biochemically or electrophysiologically to determine whether the affected cells acquire a neuronal phenotype. We have reported that low-dose staurosporine acts synergistically with cyclic AMP to induce neuronal differentiation in a human neuroblastoma cell line. This protocol describes a method for examining phenotypic changes in cultured cells.
Sasaki
K
K
Growth Factor Division, National Cancer Center Research Institute, Tokyo, Japan. ksasaki@ncc.go.jp
Maruyama
K
K
Nishimura
E
E
Tsukada
T
T
Yamaguchi
K
K
D002454:Cell Differentiation / Q000187:drug effects / Q000502:physiology; D002455:Cell Division / Q000187:drug effects; D000242:Cyclic AMP / Q000502:physiology*; D006801:Humans; D009447:Neuroblastoma / Q000473:pathology*; D009462:Neurology / Q000379:methods*; D009474:Neurons / Q000502:physiology*; D010641:Phenotype; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000378:metabolism; D019311:Staurosporine / Q000494:pharmacology*; D014158:Transcription, Genetic; D014407:Tumor Cells, Cultured / Q000187:drug effects
D012333:RNA, Messenger; D000242:Cyclic AMP; D019311:Staurosporine