Granulocyte-colony stimulating factor induces differentiation and apoptosis of CD2, CD7 positive hybrid leukemia cells in vivo and ex vivo.
We report a case of a 70-year-old man with a hybrid leukemia treated successfully with granulocyte-colony stimulating factor (G-CSF) combined with a cytocine arabinoside regimen through the induction of differentiation of leukemic cells into monocytoid cells resulting in apoptosis. The leukemic cells demonstrated a TCR-gamma rearrangement, and expressed CD2, CD7, CD33 and G-CSF receptors but not CD11b on the cell surface nor non-specific esterase in cytoplasm. Several days following the administration of G-CSF, the cells with monocytoid characteristics such as CD11b and cytoplasmic non-specific esterase appeared in the peripheral blood replacing the blastic cells. The cells were shown to be derived from the same clone of the leukemic cell because of the identical TCR-gamma gene rearrangement. The short-term culture of leukemic cells with G-CSF induced the differentiation into a monocyte lineage, resulting in apoptosis. Although there is no denying the possibility that cytosine arabinoside is partly responsible, our results strongly suggest that G-CSF plays the main role in differentiation of leukemic cells into a monocyte lineage inducing apoptosis in vivo in this patient.
Fujiwara
H
H
First Department of Internal Medicine, Faculty of Medicine, Kagoshima University, Japan.
Arima
N
N
Matsushita
K
K
Hidaka
S
S
Ohtsubo
H
H
Fukumori
J
J
Arimura
K
K
Kukita
T
T
Tanaka
H
H
D000368:Aged; D019011:Antigens, CD7 / Q000378:metabolism*; D000971:Antineoplastic Combined Chemotherapy Protocols / Q000627:therapeutic use; D017209:Apoptosis; D018801:CD2 Antigens / Q000378:metabolism*; D002454:Cell Differentiation; D003561:Cytarabine / Q000627:therapeutic use; D053938:DNA Fragmentation; D016179:Granulocyte Colony-Stimulating Factor / Q000494:pharmacology*; D006801:Humans; D015456:Leukemia, Biphenotypic, Acute / Q000188:drug therapy* / Q000473:pathology; D016131:Lymphocyte Subsets / Q000276:immunology; D008297:Male; D013997:Time Factors; D014407:Tumor Cells, Cultured
D019011:Antigens, CD7; D018801:CD2 Antigens; D003561:Cytarabine; D016179:Granulocyte Colony-Stimulating Factor