Differential regulation of fasA and fasH expression of Escherichia coli 987P fimbriae by environmental cues.
An early process in the pathogenesis of enteric bacteria is colonization of the intestinal epithelium leading to local multiplication, pathophysiological interactions with the host and further spreading. Attachment is typically mediated by bacterial fimbriae, which are selectively expressed during growth in the intestine. Here we report an analysis of the regulation of 987P fimbrial expression of enterotoxigenic Escherichia coli (ETEC). Expression of both fasH, the transcriptional activator of the 987P fimbrial genes, and fasA, the major fimbrial subunit, is regulated in response to a variety of environmental stimuli. We have found that expression of fasH is regulated in response to the carbon status of the growth medium by the cAMP-CRP complex. Moreover, fasH is regulated in response to both the nitrogen status of the growth medium and the external pH. Expression of fasA is activated by FasH, and is also selectively regulated in response to growth temperature by HNS. Regulation of fimbrial expression by carbon and/or nitrogen gradients is proposed to provide a mechanism that allows preferential colonization of different segments of the intestine by various enteropathogens, such as ETEC, enteropathogenic E. coli and Vibrio cholerae.
Edwards
R A
RA
Department of Pathobiology, University of Pennsylvania School of Veterinary Medicine, Philadelphia 19104, USA.
Schifferli
D M
DM
D018830:Adhesins, Escherichia coli / Q000235:genetics* / Q000378:metabolism; D000942:Antigens, Bacterial / Q000235:genetics* / Q000378:metabolism; D000954:Antigens, Surface / Q000235:genetics* / Q000378:metabolism; D001426:Bacterial Proteins / Q000235:genetics* / Q000378:metabolism; D002244:Carbon / Q000378:metabolism; D002455:Cell Division; D003001:Cloning, Molecular; D004926:Escherichia coli / Q000235:genetics* / Q000472:pathogenicity; D029968:Escherichia coli Proteins; D036781:Fimbriae Proteins; D010861:Fimbriae, Bacterial / Q000378:metabolism*; D015964:Gene Expression Regulation, Bacterial; D006863:Hydrogen-Ion Concentration; D009584:Nitrogen / Q000378:metabolism; D012333:RNA, Messenger / Q000378:metabolism; D013696:Temperature; D014157:Transcription Factors
D018830:Adhesins, Escherichia coli; D000942:Antigens, Bacterial; D000954:Antigens, Surface; D001426:Bacterial Proteins; D029968:Escherichia coli Proteins; C067955:FAPR protein, E coli; D012333:RNA, Messenger; D014157:Transcription Factors; C035391:antigen 987P, E coli; D036781:Fimbriae Proteins; D002244:Carbon; D009584:Nitrogen