Additional primer sets for an amelogenin gene PCR-based DNA-sex test.
When aligning the human amelogenin X (AmelX)-gene with the human amelogenin Y (AmelY)-gene, 19 regions of absolute homology ranging in size from 22 to 80 bp, 5 deletions located on AmelX from 1 to 6 bp and 5 deletions 1-183 bp long located on AmelY can be observed. The regions of absolute homology are used to design primer sets which span deletions of the X- and/or Y-chromosome. The PCR products generated on the X- and Y-homologues differ in size. New regions of deletions and newly designed primers are described to facilitate the integration of the amelogenin sex test into multiplex PCR reactions.
Haas-Rochholz
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H
Institut für Rechtsmedizin der Justus-Liebig-Universität Giessen, Germany.
Weiler
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G
D053523:Amelogenin; D004247:DNA / Q000032:analysis; D017931:DNA Primers; D003746:Dental Enamel Proteins / Q000235:genetics*; D005260:Female; D005554:Forensic Medicine / Q000379:methods*; D017353:Gene Deletion; D006801:Humans; D008297:Male; D012091:Repetitive Sequences, Nucleic Acid; D012680:Sensitivity and Specificity; D012689:Sequence Homology, Nucleic Acid; D012732:Sex Determination Analysis
C507199:AMELX protein, human; C507202:AMELY protein, human; D053523:Amelogenin; D017931:DNA Primers; D003746:Dental Enamel Proteins; D004247:DNA