Distribution and localization of a novel cholecystokinin-releasing factor in the rat gastrointestinal tract.
The purpose of this study was to examine the distribution and localization of an intestinal cholecystokinin (CCK)-releasing factor, called luminal CCK-releasing factor (LCRF), in the gastrointestinal tract and pancreas of the rat. RIA analysis indicates that LCRF immunoreactivity is found throughout the gut including the pancreas, stomach, duodenum, jejunum, ileum, and colon with the highest levels in the small intestine. Immunohistochemistry analysis shows LCRF immunoreactivity staining in intestinal villi, Brunner's glands of the duodenum, the duodenal myenteric plexus, gastric pits, pancreatic ductules, and pancreatic islets. These results indicate potential sources for secretagogue-stimulated release of luminal LCRF and support the hypothesis that LCRF is secreted into the intestinal lumen to stimulate CCK release from mucosal CCK cells.
Tarasova
N
N
Advanced Bioscience Laboratories-Basic Research Program, National Cancer Institute-Frederick Cancer Research and Development Center, Frederick, Maryland 21702, USA.
Spannagel
A W
AW
Green
G M
GM
Gomez
G
G
Reed
J T
JT
Thompson
J C
JC
Hellmich
M R
MR
Reeve
J R
JR
Liddle
R A
RA
Greeley
G H
GH
D000818:Animals; D004064:Digestive System / Q000378:metabolism*; D006133:Growth Substances / Q000378:metabolism*; D007150:Immunohistochemistry / Q000379:methods; D036341:Intercellular Signaling Peptides and Proteins; D008297:Male; D010179:Pancreas / Q000378:metabolism; D011863:Radioimmunoassay; D051381:Rats; D017207:Rats, Sprague-Dawley; D013194:Staining and Labeling; D014018:Tissue Distribution; D014359:Trypsin Inhibitor, Kazal Pancreatic
D006133:Growth Substances; D036341:Intercellular Signaling Peptides and Proteins; C504705:Spink1 protein, rat; D014359:Trypsin Inhibitor, Kazal Pancreatic