Omega-3 polyunsaturated fatty acids inhibit migration of human vascular smooth muscle cells in vitro.
Arterial smooth muscle cell migration from the media to the intima is a crucial process in the pathogenesis of atherosclerosis. Platelet-derived growth factor (PDGF) has been proposed to play a key role in the development of advanced atherosclerotic lesions by stimulating the migration and proliferation of vascular smooth muscle cells. Polyunsaturated fatty acids (PUFA) of the omega-3 series, extracted from fish oil has been shown to have beneficial effects on atherosclerosis. In this study, we evaluated the effects of omega-3 PUFA on the migration of human aortic smooth muscle cell (hASMC) in vitro. The migration assay was performed according to the Capsoni's method using transwell culture plates. PDGF, fibrinogen or 10% FCS significantly stimulated hASMC migration, however, omega-3 PUFA significantly inhibited PDGF-induced migration of hASMC. These results suggest that the inhibitory effect of omega-3 PUFA on cell migration may be an important aspect by which omega-3 PUFA exerts its antiatherosclerotic influence.
Mizutani
M
M
Department of Endocrinology and Metabolism, University of Tsukuba, Japan.
Asano
M
M
Roy
S
S
Nakajima
T
T
Soma
M
M
Yamashita
K
K
Okuda
Y
Y
D002465:Cell Movement / Q000187:drug effects; D002478:Cells, Cultured; D016895:Culture Media, Serum-Free; D004281:Docosahexaenoic Acids / Q000494:pharmacology; D015118:Eicosapentaenoic Acid / Q000494:pharmacology; D015525:Fatty Acids, Omega-3 / Q000494:pharmacology*; D005231:Fatty Acids, Unsaturated / Q000494:pharmacology; D005340:Fibrinogen / Q000494:pharmacology; D006801:Humans; D009131:Muscle, Smooth, Vascular / Q000166:cytology* / Q000187:drug effects*; D010982:Platelet-Derived Growth Factor / Q000494:pharmacology
D016895:Culture Media, Serum-Free; D015525:Fatty Acids, Omega-3; D005231:Fatty Acids, Unsaturated; D010982:Platelet-Derived Growth Factor; D004281:Docosahexaenoic Acids; D005340:Fibrinogen; D015118:Eicosapentaenoic Acid; C026219:docosapentaenoic acid