[Bovine serum albumin-conjugated corticosterone inhibits the release of arginine vasopressin].
The arginine vasopressin (AVP) released from rat hypothalamic slices containing paraventricular and supraoptic nuclei sectioned with vibratome and incubated in microchambers was measured by radioimmunoassay. The effect of bovine serum albumin-conjugated corticosterone (B-BSA) on the AVP release was investigated. The results were as follows: (1) B-BSA, within 20 min, inhibited the AVP release in a dose-dependent manner from 10(-7) to 10(-4) mol/L. (2) The inhibitory effect of B-BSA was partially blocked by RU486 (10(-4)-10(-3) mol/L). (3) With the elevation of Ca2+ in the incubation medium the inhibitory effect of B-BSA was enhanced. (4) The inhibitory effect of B-BSA was also enhanced in the presence of neomycin (10(-3)-10(-2) mol/L). These results suggested that glucocorticoid, without entering into cells, could inhibit AVP release from rat hypothalamic slices. This effect might be mediated via a non-genomic mechanism involving a change of Ca2+ influx across the cell membrane.
Liu
X
X
Department of Physiology, Second Military Medical University, Shanghai.
Wang
C A
CA
Chen
Y Z
YZ
D000818:Animals; D001127:Arginine Vasopressin / Q000378:metabolism*; D002118:Calcium / Q000378:metabolism; D002417:Cattle; D003345:Corticosterone / Q000494:pharmacology*; D005260:Female; D007031:Hypothalamus / Q000378:metabolism*; D008297:Male; D010286:Paraventricular Hypothalamic Nucleus / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D012710:Serum Albumin, Bovine / Q000494:pharmacology*; D013495:Supraoptic Nucleus / Q000378:metabolism
D001127:Arginine Vasopressin; D012710:Serum Albumin, Bovine; D002118:Calcium; D003345:Corticosterone