title: Altered N-glycosylation of glucose transporter-1 associated with radiation-induced tumorigenesis of human cell hybrids.
abstract: Studies on human cell hybrids between a cervical carcinoma cell line, HeLa, and normal fibroblasts have indicated that their tumorigenicity is under the control of a putative tumor suppressor on chromosome 11. We have previously demonstrated that a tumorigenic cell hybrid CGL4 expresses a larger glucose transporter, GLUT1, due to altered glycosylation when compared to the nontumorigenic counterpart CGL1. In this study, we demonstrated this glycosylation change in GLUT1 in gamma-ray-induced tumorigenic mutants (GIMs) isolated from CGL1 cells as expressing a tumor-associated surface antigen, intestinal alkaline phosphatase. In contrast, GLUT1 in the gamma-irradiated nontumorigenic control cells (CONs) did not show this alteration. In accordance with this glycosylation change, affinity to 2-deoxyglucose in these GIM clones was increased by about twofold when compared to the nontumorigenic CONs. These results suggest a close correlation between the glycosylation change in GLUT1 with increased affinity to D-glucose and tumorigenicity of these human cell hybrids.
authors.affiliation: Department of Biochemistry and Cell Biology, National Institute of Infectious Diseases, Tokyo, Japan.
authors.forename: Y
authors.initials: Y
authors.lastname: Noto
authors.forename: A
authors.initials: A
authors.lastname: Iwazaki
authors.forename: J
authors.initials: J
authors.lastname: Nagao
authors.forename: Y
authors.initials: Y
authors.lastname: Sumiyama
authors.forename: J L
authors.initials: JL
authors.lastname: Redpath
authors.forename: E J
authors.initials: EJ
authors.lastname: Stanbridge
authors.forename: T
authors.initials: T
authors.lastname: Kitagawa
mesh_terms: D000469:Alkaline Phosphatase / Q000378:metabolism; D001692:Biological Transport; D002471:Cell Transformation, Neoplastic / Q000528:radiation effects*; D003847:Deoxyglucose / Q000378:metabolism; D005347:Fibroblasts; D005720:Gamma Rays; D051272:Glucose Transporter Type 1; D006031:Glycosylation / Q000528:radiation effects; D006367:HeLa Cells; D006801:Humans; D006822:Hybrid Cells; D007700:Kinetics; D009004:Monosaccharide Transport Proteins / Q000737:chemistry / Q000378:metabolism* / Q000528:radiation effects; D009844:Oligosaccharides / Q000737:chemistry / Q000302:isolation & purification
chemical_list: D051272:Glucose Transporter Type 1; D009004:Monosaccharide Transport Proteins; D009844:Oligosaccharides; C495677:SLC2A1 protein, human; D003847:Deoxyglucose; D000469:Alkaline Phosphatase