[Protective effects of beta-carotene liposome against rat neutrophile membrane damage caused by intra- or extra-cellular reactive oxygen species].
In this experiment the protective effects of beta-carotene (beta-C) liposome against rat neutrophile membrane damage caused by extracellular or intracellular reactive oxygen were species (ROS) investigated by means of fluorescence labels 1, 6-diphenyl-1,3,5 hexatriane (DPH) and N-(3-pyrene) maleimide (N-(3p)M). The extracellular ROS include the singlet oxygen (1O2) generated by photoreaction of sensitizer rosebengal and the superoxide anion (O2-.) produced by xanthine/xanthine oxidase reactive system. The intracellular ROS was sensitizer rosebengal and superoxide anion (O2-.) produced by xanthine/xanthine oxidase reactive system while the intracellular oxygen was generated by stimulating neutrophile respiratory burst with opsonized zymosan. No change in the peak or shape of fluorescent excitation and emission spectra was found for either of the two labels. The results of fluorescent polarization value in the membrane showed that beta-C liposome could significantly inhibit the damage of rat neutrophile membrane protein reacted either by intracellular generated reactive oxygen species or by extracellular generated O2-. or 1O2. It could also inhibit the injury of the rat neutrophile membrane lipid reacted by extracellular reactive oxygen species. However, the protective effects of beta-C liposome against membrane lipid damage reacted by intracellular reactive oxygen species by at the same concentration as those on membrane protein were not significant, which could be partly explained by relatively higher damage level of rat neutrophile membrane lipid domain reacted by intracellular reactive oxygen species than that of the membrane protein domain.
Jiang
J
J
Institute of Basic Medical Sciences, CAMS, Beijing.
Yu
Z
Z
Qiu
Q
Q
Wu
Y
Y
D000818:Animals; D000975:Antioxidants / Q000494:pharmacology*; D005609:Free Radicals; D008563:Membrane Lipids / Q000378:metabolism; D008565:Membrane Proteins / Q000378:metabolism; D009504:Neutrophils / Q000187:drug effects*; D010100:Oxygen / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D016897:Respiratory Burst / Q000187:drug effects; D019207:beta Carotene / Q000494:pharmacology*
D000975:Antioxidants; D005609:Free Radicals; D008563:Membrane Lipids; D008565:Membrane Proteins; D019207:beta Carotene; D010100:Oxygen