title: [Impairment in the phosphorylation of Ca(2+)-transport ATPase of rat liver endoplasmic reticulum during sepsis].
abstract: The phosphorylation of Ca(2+)-transport ATPase of rat liver endoplasmic reticulum (ER) during early and late septic shock induced by cecum ligation and puncture (CLP) was investigated by determining incorporation of [gamma-32P] ATP into Ca(2+)-ATP phosphoprotein intermediate. Hepatic endoplasmic reticulum was isolated by differential centrifugation with sucrose density gradient. The Ca(2+)-ATPase phosphoprotein intermediate was identified by SDS-PAGE. The results showed that the phosphorylation of Ca(2+)-ATPase (115 kD) was decreased respectively by 15-23% (P < 0.05) and 17-27% (P < 0.05) at 9 h (early sepsis) and 18 h (late sepsis), following the CLP in the rough, intermediate and smooth ER preparations. Kinetic analysis using rough ER showed that the Vmax for Ca2+ and for ATP for the phosphorylation of Ca(2+)-ATPase were decreased dramatically during early and late sepsis, but without changes in the K(m) values. These results demonstrate that the phosphorylation of the phosphoprotein intermediate of Ca(2+)-ATPase in rat liver was impaired during different phases of sepsis.
authors.affiliation: Laboratory of Shock Research, Beijing Medical University.
authors.forename: A G
authors.initials: AG
authors.lastname: Gao
authors.forename: L W
authors.initials: LW
authors.lastname: Dong
authors.forename: X Q
authors.initials: XQ
authors.lastname: Wang
authors.forename: N K
authors.initials: NK
authors.lastname: Liu
authors.forename: C S
authors.initials: CS
authors.lastname: Tang
authors.forename: M S
authors.initials: MS
authors.lastname: Liu
mesh_terms: D000818:Animals; D000252:Calcium-Transporting ATPases / Q000378:metabolism*; D004721:Endoplasmic Reticulum / Q000201:enzymology; D008099:Liver / Q000201:enzymology*; D008297:Male; D010766:Phosphorylation; D051381:Rats; D017207:Rats, Sprague-Dawley; D012772:Shock, Septic / Q000378:metabolism*
chemical_list: D000252:Calcium-Transporting ATPases