The effect of heparin on the activity of Trichosporon cutaneum casein kinase I.
Casein kinase I from Trichosporon cutaneum ribosome-free extracts was purified. Its molecular mass was calculated for 33 kDa. It was shown that casein, phosvitin and Trichosporon cutaneum ribosomal protein of 15 kDa were preferable substrates for the enzyme. It was found that heparin can stimulate or inhibit CKI activity depending on the substrate used. Stimulation of casein and inhibition of phosvitin phosphorylation was observed. In addition it was shown that ribosomal proteins of 19 kDa and 38 kDa were phosphorylated by CKI only in the presence of heparin.
Wojda
I
I
Department of Molecular Biology, Institute of Microbiology and Biotechnology, Maria Curie-Skłodowska University, Akademicka, Poland. iwoda@biotop.umcs.lublin.pl
Frajnt
M
M
Jakubowicz
T
T
D047388:Casein Kinases; D006493:Heparin / Q000494:pharmacology*; D008970:Molecular Weight; D010766:Phosphorylation; D011494:Protein Kinases / Q000187:drug effects*; D014250:Trichosporon / Q000201:enzymology*
D006493:Heparin; D011494:Protein Kinases; D047388:Casein Kinases