Basic neural transplantation techniques. I. Dissociated cell suspension grafts of embryonic ventral mesencephalon in the adult rat brain.
Lesions and grafts in the nigrostriatal dopamine system in rats is widely used as a model of degeneration and regeneration in the CNS, and the development for alternative strategies for treatment in Parkinson's disease. The methods of preparing a dissociated cell suspension of embryonic rat substantia nigra and its transplantation into the brain of adult rats are described. This is the first of a series of methodological reports on the basic methodology and refinements of neural transplantation techniques in the mammalian central nervous system.
Dunnett
S B
SB
MRC Cambridge Centre for Brain Repair, University of Cambridge, UK. sd19@cam.ac.uk
Björklund
A
A
D000818:Animals; D001921:Brain / Q000378:metabolism / Q000601:surgery*; D017690:Cell Transplantation / Q000379:methods*; D003342:Corpus Striatum / Q000378:metabolism / Q000601:surgery; D004298:Dopamine / Q000378:metabolism; D005260:Female; D016332:Fetal Tissue Transplantation / Q000379:methods*; D006085:Graft Survival; D008297:Male; D008636:Mesencephalon / Q000166:cytology / Q000196:embryology*; D009417:Nerve Tissue / Q000166:cytology / Q000196:embryology*; D009488:Neurosciences / Q000379:methods*; D051381:Rats / Q000196:embryology; D013378:Substantia Nigra / Q000166:cytology / Q000196:embryology; D014446:Tyrosine 3-Monooxygenase / Q000378:metabolism
D014446:Tyrosine 3-Monooxygenase; D004298:Dopamine