title: Dietary triglycerides, up to 40 g/meal, do not affect preformed vitamin A bioavailability in humans.
abstract: OBJECTIVE
  Assessing the effect of the dose of dietary triglycerides on preformed vitamin A (retinyl esters) bioavailability in humans.
  
  
  METHODS
  Four test meals containing 15,000 RE retinyl-palmitate and either 0, 15, 30 or 40 g added triglycerides were ingested by eight healthy volunteers, at different days and in a randomized order.
  
  
  METHODS
  The study was done in the Hospital Sainte Marguerite, Marseille, France.
  
  
  METHODS
  Eight healthy male volunteers were recruited by advertisement.
  
  
  METHODS
  Blood samples were collected every hour for seven hours after the test meals intake. Serum and chylomicron (Svedberg flotation unit > 1000) were prepared by centrifugation and retinyl esters were measured by HPLC.
  
  
  RESULTS
  The serum retinyl ester response was not significantly lower after the intake of the meal without added triglycerides (7944 +/- 3262 nmol/L h) than after the intake of the fat meals (10012 +/- 2182, 7869 +/- 3157 and 10777 +/- 2067 nmol/L h for the 15, 30 and 40 g-fat meal, respectively), indicating that the serum retinyl ester response was not related to the amount of meal triglycerides. Chylomicron retinyl linoleate response stepwise increased when the amount of meal triglycerides increased while retinyl palmitate and retinyl stearate responses reached a maximum since 15 g triglycerides. Postprandial serum retinol concentration did not change whatever the meal ingested.
  
  
  CONCLUSIONS
  (i) a significant amount of preformed vitamin A is apparently absorbed when ingested with trace amount of meal triglycerides only; (ii) meal triglycerides, up to 40 g/meal, do not increase preformed vitamin A bioavailability; (iii) the retinyl ester pattern recovered in the chylomicrons, and probably the esterification process of retinol, is affected by the amount of meal triglycerides; (iv) postprandial retinol homeostasis is not affected by dietary triglycerides.
authors.affiliation: Unité des Maladies Métaboliques et des Micronutriments, INRA (National Institute of Agronomic Research), Clermont-Ferrand, France.
authors.forename: P
authors.initials: P
authors.lastname: Borel
authors.forename: C
authors.initials: C
authors.lastname: Dubois
authors.forename: N
authors.initials: N
authors.lastname: Mekki
authors.forename: P
authors.initials: P
authors.lastname: Grolier
authors.forename: A
authors.initials: A
authors.lastname: Partier
authors.forename: M C
authors.initials: MC
authors.lastname: Alexandre-Gouabau
authors.forename: D
authors.initials: D
authors.lastname: Lairon
authors.forename: V
authors.initials: V
authors.lastname: Azais-Braesco
mesh_terms: D000328:Adult; D001682:Biological Availability; D002851:Chromatography, High Pressure Liquid; D002914:Chylomicrons / Q000378:metabolism; D004032:Diet; D004305:Dose-Response Relationship, Drug; D006801:Humans; D008297:Male; D019518:Postprandial Period; D014280:Triglycerides / Q000008:administration & dosage / Q000494:pharmacology*; D014801:Vitamin A / Q000097:blood / Q000493:pharmacokinetics*
chemical_list: D002914:Chylomicrons; D014280:Triglycerides; D014801:Vitamin A