title: Frequency selectivity on aspirin-induced hearing loss in rats with auditory stimulus-induced conditioned suppression.
abstract: The conditioned suppression technique was employed to examine the acute effects of aspirin on auditory function in rats. Lever pressing behavior for water reinforcement was suppressed in the presence of an auditory stimulus that had been previously paired with electric shocks. A single intravenous injection of aspirin at a dose of 225 mg/kg caused an erroneous lever pressing response in the broad sound intensities of 2 kHz tone stimulus during the conditioned stimulus period. A statistically significant increase in the threshold for 2 kHz was found 1 to 72 hr after dosing but not for 4, 8 and 10 kHz. These results suggest that the hearing for low sound frequency in rats is vulnerable to the effects of aspirin. This paradigm in rats may be useful to further assess the different outer hair cells along the cochlear duct and provide an additional evidence for the aspirin ototoxicity research.
authors.affiliation: Drug Safety Research Laboratories, Takeda Chemical Industries, Ltd., Osaka, Japan.
authors.forename: K
authors.initials: K
authors.lastname: Kurata
authors.forename: N
authors.initials: N
authors.lastname: Nishida
authors.forename: R
authors.initials: R
authors.lastname: Tsukuda
authors.forename: T
authors.initials: T
authors.lastname: Suzuki
authors.forename: S
authors.initials: S
authors.lastname: Sato
authors.forename: M
authors.initials: M
authors.lastname: Tokuriki
mesh_terms: D000161:Acoustic Stimulation; D000818:Animals; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000008:administration & dosage / Q000009:adverse effects* / Q000494:pharmacology; D001241:Aspirin / Q000008:administration & dosage / Q000009:adverse effects* / Q000494:pharmacology; D001309:Auditory Threshold / Q000187:drug effects; D003214:Conditioning, Classical / Q000502:physiology*; D004195:Disease Models, Animal; D004305:Dose-Response Relationship, Drug; D004558:Electric Stimulation; D016057:Evoked Potentials, Auditory, Brain Stem / Q000502:physiology; D018072:Hair Cells, Auditory, Outer / Q000502:physiology; D006309:Hearing / Q000187:drug effects / Q000502:physiology; D034381:Hearing Loss / Q000139:chemically induced / Q000503:physiopathology / Q000662:veterinary*; D007275:Injections, Intravenous; D008297:Male; D051381:Rats; D017208:Rats, Wistar; D013047:Specific Pathogen-Free Organisms
chemical_list: D000894:Anti-Inflammatory Agents, Non-Steroidal; D001241:Aspirin