Effect of ondansetron on Salmonella typhimurium-induced net fluid accumulation in the pig jejunum in vivo.
Two major pathophysiological mechanisms explaining the diarrhoea induced by Salmonella typhimurium have been suggested to be: (a) invasion of the intestine by the bacteria, and (b) an enterotoxin resembling Vibrio cholerae toxin. Cholera toxin is a potent secretagogue in pig small intestine and induces secretion partly by activating 5-hydroxytryptamine receptors, following release of 5-hydroxytryptamine. Ondansetron is a selective 5-hydroxytryptamine-3 receptor antagonist, which reduces the cholera toxin-evoked fluid accumulation in pig jejunum. The aim of this study was to investigate the effect of ondansetron on Salmonella typhimurium-induced fluid accumulation in ligated loops of pig jejunum in vivo. 10(10) colony-forming units of the bacteria was injected into loops and incubated for 8 hr. 200 mg x kg-1 ondansetron given subcutaneously reduced the Salmonella typhimurium-induced fluid accumulation by about 40%. This results suggests the involvement of 5-hydroxytryptamine and 5-hydroxytryptamine-3 receptors in Salmonella typhimurium-induced diarrhoea.
Jensen
G M
GM
Department of Physiology and Anatomy, Royal Veterinary and Agricultural University, Frederiksberg, Denmark.
Grøndahl
M L
ML
Nielsen
C G
CG
Skadhauge
E
E
Olsen
J E
JE
Hansen
M B
MB
D000818:Animals; D001826:Body Fluids / Q000378:metabolism*; D005260:Female; D066298:In Vitro Techniques; D007583:Jejunum / Q000187:drug effects* / Q000378:metabolism; D017294:Ondansetron / Q000494:pharmacology*; D012486:Salmonella typhimurium / Q000502:physiology*; D013552:Swine / Q000378:metabolism*
D017294:Ondansetron