[Expressing poliovirus (type I) and hepatitis A virus antigens by recombinant vaccinia virus].
A recombinant vaccinia virus expressing PV-I and HAV antigens was constructed. Dot blot and Southern blot confirmed that the recombinant virus genome contained PV-I cDNA and HAV cDNA chimeric fragment. The result of IF indicated that PV-I antigen and HAV antigen were successfully expressed in the mammalian cells infected with the recombinant virus. The expressed PV-I and HAV antigen showed one band which had both PV-I and HAV antigenicities in Western blot. The band was about 120,000(MW). When guinea pigs were immunized with the recombinant virus, PV-I and HAV neutralizing antibodies were induced. The result indicated that the recombinant virus constructed here had bi-valence of PV-I and HAV antigen as well as PV-I and HAV immunogenecities.
Liu
J
J
Institute of Medical Biology, CAMS, Kuming.
Su
Y
Y
Dai
C
C
Guo
R
R
D000818:Animals; D000914:Antibodies, Viral / Q000096:biosynthesis; D000956:Antigens, Viral / Q000032:analysis; D006168:Guinea Pigs; D017957:Hepatitis A Virus, Human / Q000235:genetics*; D017955:Poliovirus / Q000235:genetics*; D011993:Recombinant Fusion Proteins / Q000235:genetics*; D014616:Vaccinia virus / Q000235:genetics*
D000914:Antibodies, Viral; D000956:Antigens, Viral; D011993:Recombinant Fusion Proteins