[Establishment of human amnion cell mutagenesis system by using a shuttle vector pS189].
Using transient shuttle vector pS189, we established a subclone human amnion cell (FL) mutagenesis detection system, and we detected the mutation specificity of N-methy-N'-nitro-N-nitrosoguanidine (MNNG) in this system. The spontaneous mutant frequency of target gene supF was 1.7 x 10(-5) and MNNG-induced mutant frequency was increased with dosage. The results from 0.8% agarose gel electrophoresis, PCR, PCR-single strand conformation polymorphism (PCR-SSCP) analysis showed that MNNG-induced 89% mutants were point mutants. These results indicated that this system can be used to detect and study the mutagenesis mechanism of potential mutagens.
Wang
H
H
Yi
S
S
Xiao
Y
Y
Li
D
D
Qi
J
J
Fu
J
J
D000650:Amnion / Q000166:cytology*; D002478:Cells, Cultured; D005260:Female; D005822:Genetic Vectors; D006801:Humans; D008769:Methylnitronitrosoguanidine; D009152:Mutagenicity Tests; D009153:Mutagens; D017354:Point Mutation / Q000187:drug effects; D016133:Polymerase Chain Reaction; D018807:Polymorphism, Single-Stranded Conformational
D009153:Mutagens; D008769:Methylnitronitrosoguanidine