Orotic acid in clinical urine specimens by capillary zone electrophoresis using polyvinyl alcohol coated capillaries.
Orotic acid is an important compound in the investigation of several inherited metabolic diseases, and capillary zone electrophoresis provides an effective assay method for this analyte in the clinical laboratory setting. Capillaries with a polyvinyl alcohol (PVA) coating performed well in this application. However, it was necessary to employ several specimen preparation steps to achieve good assay precision.
Franke
D R
DR
ARUP Laboratories, Salt Lake City, UT 84108, USA.
Nuttall
K L
KL
D019075:Electrophoresis, Capillary / Q000295:instrumentation / Q000379:methods; D006801:Humans; D007202:Indicators and Reagents; D009963:Orotic Acid / Q000652:urine*; D011142:Polyvinyl Alcohol; D015203:Reproducibility of Results
D007202:Indicators and Reagents; D009963:Orotic Acid; D011142:Polyvinyl Alcohol