Microglia and astroglia have a common progenitor cell.
Disaggregated neopallial cells from newborn C3H/HeJ mice were cloned in Grenier hybridoma tissue culture dishes, and culture wells that contained only one cell were marked. After 8-10 days of culturing, the cultures were fixed and double immunolabeled for microglia with Mac-1 antibody and for astroglia with antibody to GFAP. Each marked well containing a clone was identified as either a microglia, astroglia, mixed microglia-astroglia, or an unlabeled clone. The effect of LM cell line conditioned medium (LM-CM), which contains colony-stimulating factor-1, on the development of mixed microglia-astroglia clones was determined. Formation of mixed clones was dose dependent (P < 0.0001). We concluded that microglia and astroglia have a common progenitor cell and that the development of mixed clones is LM-CM dependent.
Fedoroff
S
S
Department of Anatomy and Cell Biology, University of Saskatchewan, Saskatoon, Canada. fedoroff@sask.usask.ca
Zhai
R
R
Novak
J P
JP
D000818:Animals; D000831:Animals, Newborn; D001253:Astrocytes / Q000166:cytology*; D015415:Biomarkers; D002454:Cell Differentiation; D002478:Cells, Cultured; D002999:Clone Cells; D017077:Culture Media, Conditioned; D005904:Glial Fibrillary Acidic Protein / Q000032:analysis; D007739:L Cells; D016177:Macrophage-1 Antigen / Q000032:analysis; D051379:Mice; D008809:Mice, Inbred C3H; D017628:Microglia / Q000166:cytology*; D019579:Neocortex / Q000166:cytology* / Q000502:physiology; D013234:Stem Cells / Q000166:cytology / Q000502:physiology*
D015415:Biomarkers; D017077:Culture Media, Conditioned; D005904:Glial Fibrillary Acidic Protein; D016177:Macrophage-1 Antigen