Structural and functional roles of modules in hemoglobin. Substitution of module M4 in hemoglobin subunits.
The alpha- and beta-subunits of human hemoglobin consist of the modules M1, M2 + M3, and M4, which correspond to the exons 1, 2, and 3, respectively (Go, M. (1981) Nature 291, 90-92). To gain further insight into functional and structural significance of the modules, we designed two kinds of chimeric hemoglobin subunits (chimeric alphaalphabeta- and betabetaalpha-subunits), in which the module M4 was replaced by the partner subunits. CD spectra in the far-UV region showed that the secondary structure of the chimeric alphaalphabeta-subunit drastically collapsed, while the chimeric betabetaalpha-subunit conserved the native globin structure (Wakasugi, K., Ishimori, K., Imai, K., Wada, Y., and Morishima, I. (1994) J. Biol. Chem. 269, 18750-18756). SAXS data also suggested a partially disordered structure of the chimeric alphaalphabeta-subunit. Based on tryptophan fluorescence spectra and computer modeling from x-ray structures of native globins, steric constraint between Trp14 and Tyr125 would be induced in the chimeric alphaalphabeta-subunit, which would perturb the packing of the A- and H-helices and destabilize the globule structure. On the other hand, such a steric constraint was not found for the counterpart chimeric subunit, the betabetaalpha-subunit. The different stabilities of these module-substituted globins imply that modules would not always be stable "structural" units, and interactions between modules are crucial to construct stable globin subunits.
Inaba
K
K
Department of Molecular Engineering, Graduate School of Engineering, Kyoto University, Kyoto 606, Japan. morisima@mds.moleng.kyoto-u.ac.jp
Wakasugi
K
K
Ishimori
K
K
Konno
T
T
Kataoka
M
M
Morishima
I
I
D002942:Circular Dichroism; D006418:Heme / Q000737:chemistry; D006454:Hemoglobins / Q000737:chemistry*; D006801:Humans; D008970:Molecular Weight; D011487:Protein Conformation; D011489:Protein Denaturation; D017433:Protein Structure, Secondary; D011993:Recombinant Fusion Proteins / Q000737:chemistry; D012542:Scattering, Radiation; D013053:Spectrophotometry; D013329:Structure-Activity Relationship; D014965:X-Rays
D006454:Hemoglobins; D011993:Recombinant Fusion Proteins; D006418:Heme