[Separation of the ventral disc proteins of Giardia intestinalis].
We used repeat isoelectric focusing to obtain cytoskeleton proteins of Giardia intestinalis with the characteristics of giardins and tubulins of the ventral disk. Their molecular weight and its variants by pH difference ranged between 30 and 45 kDa. Polypeptides with molecular weights between 97 and 115 kDa were also obtained. Our immunocytochemical studies showed that the proteins with high molecular weight were from the membrane of the trophozoites; in a smaller proportion we observed also proteins with molecular weights similar to those of giardins and tubulins.
Jiménez-Cardoso
E
E
Lab. Investigación en Parasitología, Hospital Infantil de México Federico Gómez, México, D.F.
Crisóstomo-Vázquez
P
P
Suárez-Souto
A
A
D000818:Animals; D003598:Cytoskeletal Proteins / Q000302:isolation & purification*; D019084:Fluorescent Antibody Technique, Indirect; D016829:Giardia lamblia / Q000737:chemistry* / Q000254:growth & development / Q000648:ultrastructure; D006863:Hydrogen-Ion Concentration; D007124:Immunoenzyme Techniques; D007525:Isoelectric Focusing; D008565:Membrane Proteins / Q000302:isolation & purification; D008970:Molecular Weight; D015800:Protozoan Proteins / Q000302:isolation & purification*; D014404:Tubulin / Q000302:isolation & purification
D003598:Cytoskeletal Proteins; D008565:Membrane Proteins; D015800:Protozoan Proteins; D014404:Tubulin; C038207:giardin protein, Giardia lamblia