Alterations of intracellular calcium concentration in mice neuroblastoma cells by electrical field and UVA.
By using a FURA2 ratio imaging method, the intracellular free calcium concentration was investigated in cultured mice neuroblastoma cells under the influence of an amplitude-modulated (AM) field (5 kHz sine wave AM 16 Hz sinusoidal 800 V/m and 80 V/m), as well as of electric field pulses (300-ms unipolar pulses of 1000 V/m and 800 V/m, 5 pulses during 10 s and 50 pulses during 100 s). An increase in free intracellular calcium was found in about 50% of cells after field application, whereas in control experiments only about 20% of the cells showed similar increases. However, this effect depended on the amount of UV irradiation used for excitation of FURA2 fluorescence. Experiments with 1/30 to former total illumination no longer demonstrated an increase in control cells or in cells treated with AM fields. The number of cells showing calcium increase after the application of pulsed fields was reduced significantly. Therefore, the UV light itself, applied as double flashes for the fluorescence measurement, activates the cellular calcium regulation. These findings offer a possible explanation for the low reproducibility of field effects found in different laboratories, in which investigations were performed with different equipment using different intensities of UV excitation.
Ihrig
I
I
Institute of Biology, Humboldt University, Berlin, Germany.
Heese
C
C
Glaser
R
R
D000818:Animals; D002118:Calcium / Q000378:metabolism* / Q000528:radiation effects*; D004574:Electromagnetic Fields; D007424:Intracellular Fluid / Q000378:metabolism* / Q000528:radiation effects; D051379:Mice; D009447:Neuroblastoma / Q000378:metabolism*; D013997:Time Factors; D014407:Tumor Cells, Cultured; D014466:Ultraviolet Rays
D002118:Calcium