Genetic analysis of equine methicillin-resistant Staphylococcus aureus by pulsed-field gel electrophoresis.
Pulsed-field gel electrophoresis (PFGE) was used to determine genetic relationships among 15 methicillin-resistant Staphylococcus aureus (MRSA) isolates from mares with metritis and from a stallion with dermatitis in Hokkaido. All the 15 isolates showed phage pattern 6/47/54/75, coagulase type IV, and enterotoxin type A. The restriction endonuclease SmaI cut their genomic DNAs into 15 or 16 fragments ranging in size from 8 to 630 kb. Fourteen of the 15 isolates showed the same PFGE pattern, whereas the remaining one appeared to be closely related. The 9 human MRSA isolates showing the same phenotypic characteristics as the horse isolates gave different PFGE patterns from those of the horse isolates.
Shimizu
A
A
Department of Microbiology and Immunology, Faculty of Agriculture, Kobe University, Hyogo, Japan.
Kawano
J
J
Yamamoto
C
C
Kakutani
O
O
Anzai
T
T
Kamada
M
M
D000818:Animals; D004269:DNA, Bacterial / Q000032:analysis / Q000737:chemistry / Q000235:genetics*; D003872:Dermatitis / Q000382:microbiology / Q000473:pathology / Q000662:veterinary; D016521:Electrophoresis, Gel, Pulsed-Field / Q000379:methods / Q000662:veterinary*; D004716:Endometritis / Q000382:microbiology / Q000473:pathology / Q000662:veterinary; D005260:Female; D006734:Horse Diseases / Q000382:microbiology* / Q000473:pathology; D006736:Horses; D006801:Humans; D008297:Male; D016106:Methicillin Resistance / Q000235:genetics*; D010641:Phenotype; D012867:Skin / Q000382:microbiology / Q000473:pathology; D013203:Staphylococcal Infections / Q000382:microbiology / Q000473:pathology / Q000662:veterinary*; D013211:Staphylococcus aureus / Q000187:drug effects* / Q000235:genetics* / Q000302:isolation & purification; D014599:Uterus / Q000382:microbiology / Q000473:pathology
D004269:DNA, Bacterial