[Observations of cellular differentiation behaviour in adenoid cystic carcinoma of human salivary gland in vitro].
The celluar differentiation behaviour of adenoid cystic carcinoma (ACC) of salivary gland was investigated in vitro with immunohistochemistry. The results showed that the cells of ACC-2 and ACC-3 cell lines can express some marker proteins in vitro including S-100 protein, actin, myosin which is characterized as a feature of myoepithelial cells. When differentiation inducer, dB-cAMP, was added to the monolayer cells cultured in dishes, the marker protein expression increased and the CEA changed to positive. The cellular differetiation behaviour of ACC cells is related to the proliferate speed of cells. Some cells manifested finger ring-like when the culture condition was changed, it means that these cells have the secretory function. If the ACC cells were suspended singly in collagen gels which performed as an extracellular matrix they grew and formed the tubule-like structure. The results will help us to understand the histogenesis of ACC, the source of myoepithelial cells in ACC and the variety of histological structures of ACC.
He
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R
School of Stomatology, Shanghai Second Medical University.
Zhou
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X
Xia
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W
D000199:Actins / Q000096:biosynthesis; D003528:Carcinoma, Adenoid Cystic / Q000473:pathology*; D002454:Cell Differentiation; D006801:Humans; D007150:Immunohistochemistry; D009218:Myosins / Q000096:biosynthesis; D010307:Parotid Neoplasms / Q000473:pathology*; D009418:S100 Proteins / Q000096:biosynthesis; D012468:Salivary Gland Neoplasms / Q000473:pathology*; D012470:Salivary Glands, Minor; D014407:Tumor Cells, Cultured
D000199:Actins; D009418:S100 Proteins; D009218:Myosins