Molecular typing of methicillin-resistant Staphylococcus aureus by polymerase chain reaction: distribution of the typed strains in hospitals.
Three methods for molecular typing of methicillin-resistant Staphylococcus aureus using polymerase chain reaction (PCR) were compared. The method which amplified the variable number of tandem repeat of dru sequences grouped the isolates into six types. Whereas, the method examining restriction fragment length polymorphism of coagulase gene and the method using arbitrarily primed-PCR showed poor diversity in typing. We investigated the distribution of dru types in two hospitals. Obvious concentration of a type in one ward was not recognized in our hospital. In the other hospital, a rare type was detected from the inpatients in the pediatrics ward. It suggested that the infection was an epidemic. We also found that some patients were infected with more than two strains. Even if two isolates show the same type, it does not necessarily mean that they originated from one clone. However, this method brings meaningful information on nosocomial infection, more easily than other genotyping.
Tohda
S
S
Department of Laboratory Medicine, Tokyo Medical and Dental University.
Maruyama
M
M
Nara
N
N
D015373:Bacterial Typing Techniques; D003428:Cross Infection / Q000382:microbiology; D017931:DNA Primers / Q000737:chemistry; D004269:DNA, Bacterial / Q000032:analysis; D005838:Genotype; D006769:Hospitals, General; D006801:Humans; D007564:Japan / Q000453:epidemiology; D008712:Methicillin / Q000494:pharmacology*; D016106:Methicillin Resistance; D018598:Minisatellite Repeats / Q000187:drug effects / Q000235:genetics; D010406:Penicillins / Q000494:pharmacology*; D016133:Polymerase Chain Reaction / Q000379:methods*; D012150:Polymorphism, Restriction Fragment Length; D011897:Random Allocation; D012189:Retrospective Studies; D013203:Staphylococcal Infections / Q000382:microbiology; D013211:Staphylococcus aureus / Q000145:classification* / Q000187:drug effects / Q000235:genetics
D017931:DNA Primers; D004269:DNA, Bacterial; D010406:Penicillins; D008712:Methicillin