Polypropylene fibre web, a new matrix for sampling blood for immunodiagnosis of schistosomiasis.
Blood sampling on filter paper is widely used for immunodiagnostic and epidemiological purposes. However, elution of conventional filter papers impregnated with sera containing Schistosoma mansoni circulating anodic antigen (CAA) recovered only a small fraction of the antigen, thereby reducing the sensitivity of the assay. Polypropylene-based non-woven fibre web is a new sampling material with a low density of fibres and with a small surface area of contact. When it was impregnated with serum containing CAA, approximately 90% of the antigen could be extracted. The yield of antibodies against S. mansoni from the new sampling material did not differ from that from conventional filter papers.
Jamaly
S
S
Department of Medical Microbiology and Immunology, University of Göteborg, Sweden.
Chihani
T
T
Deelder
A M
AM
Gabone
R
R
Nilsson
L A
LA
Ouchterlony
O
O
D000818:Animals; D000909:Antibodies, Helminth / Q000097:blood; D001800:Blood Specimen Collection / Q000295:instrumentation*; D006801:Humans; D007159:Immunologic Tests; D008854:Microscopy, Electron; D011126:Polypropylenes; D012550:Schistosoma mansoni / Q000276:immunology; D012552:Schistosomiasis / Q000097:blood / Q000175:diagnosis*
D000909:Antibodies, Helminth; D011126:Polypropylenes