[Thermostable Yersinia pseudotuberculosis toxin: its isolation, purification and the characteristics of its properties].
Heat-stable toxin, lethal for mice when administered parenterally (LD50 was equal to 4.5 micrograms/mouse), was isolated from Y. pseudotuberculosis grown at a temperature of 8-10 degrees C. The toxin was found to be species-specific protein with a molecular weight of 45 kD, resistant to the action of elevated temperature (boiling for 5 minutes), 0.1-1.0% anionic detergent (sodium dodecyl sulfate) and stable at the pH range between 6.0-8.0. The amino acid sequence of the N-end fragment of the toxin was determined: H2N-Ala-Glu-Val-Asp-Ser-Ser-Glu-Phen-Pro. The toxin was inactivated after treatment with trypsin, chemotrypsin, papain. Antibodies to this toxin were detected in the blood sera of pseudotuberculosis patients.
Nedashkovskaia
E P
EP
Timchenko
N F
NF
Besednov
A L
AL
Vertiev
Iu V
IuV
D000595:Amino Acid Sequence; D000818:Animals; D001427:Bacterial Toxins / Q000032:analysis / Q000737:chemistry / Q000276:immunology / Q000302:isolation & purification* / Q000633:toxicity; D004355:Drug Stability; D006863:Hydrogen-Ion Concentration; D007114:Immunization; D007928:Lethal Dose 50; D051379:Mice; D008969:Molecular Sequence Data; D011817:Rabbits; D013696:Temperature; D015011:Yersinia pseudotuberculosis / Q000276:immunology / Q000472:pathogenicity
D001427:Bacterial Toxins