title: Characteristics of two major lectins from mungbean (Vigna radiata) seeds.
abstract: Two major lectins, MBL-I and MBL-II, were purified from Vigna radiata L. seeds using ion-exchange and gel filtration chromatography techniques. MBL-I was found to be a tetramer with native M.W. of 132 kDa and subunit M.W. of 33 kDa having alpha-galactosidase activity. MBL-II consisted of two monomeric lectins with M.W. of 94 kDa and 89 kDa which were associated mainly with beta-galactosidase activity. Both MBL-I and MBL-II are D-galactose-specific lectins.
authors.affiliation: Nuclear Agriculture Division, Bhabha Atomic Research Centre, Mumbai, India.
authors.forename: K N
authors.initials: KN
authors.lastname: Suseelan
authors.forename: C R
authors.initials: CR
authors.lastname: Bhatia
authors.forename: R
authors.initials: R
authors.lastname: Mitra
mesh_terms: D002850:Chromatography, Gel; D002852:Chromatography, Ion Exchange; D007887:Fabaceae / Q000737:chemistry*; D006863:Hydrogen-Ion Concentration; D037102:Lectins / Q000737:chemistry / Q000302:isolation & purification* / Q000378:metabolism; D037121:Plant Lectins; D010940:Plant Proteins / Q000737:chemistry / Q000302:isolation & purification* / Q000378:metabolism; D010946:Plants, Medicinal; D012639:Seeds; D000519:alpha-Galactosidase / Q000378:metabolism*; D001616:beta-Galactosidase / Q000378:metabolism*
chemical_list: D037102:Lectins; D037121:Plant Lectins; D010940:Plant Proteins; D000519:alpha-Galactosidase; D001616:beta-Galactosidase