Lysyl oxidase, cellular senescence and tumor suppression.
Replicative senescence may provide a mechanism of tumor suppression and tumor suppressor genes of the extracellular matrix, like lysyl oxidase, may play a role in cellular senescence. To test this hypothesis and determine whether the extracellular matrix may serve as a marker, the steady-state levels of human lysyl oxidase, alpha-I type III collagen and beta-actin transcripts were assessed in various cell lines during in vitro passage. Northern hybridization analysis showed a significant increase in the levels of progeria fibroblast extracellular matrix mRNAs immediately preceding senescence. The levels of these mRNAs were unaffected in age-matched normal fibroblast and fetal fibroblast cell lines.
Sharma
R
R
Department of Obstetrics and Gynecology, Wayne State University School of Medicine, Detroit, MI 48201, USA.
Kramer
J A
JA
Krawetz
S A
SA
D000199:Actins / Q000235:genetics / Q000378:metabolism; D000328:Adult; D002460:Cell Line; D016922:Cellular Senescence; D003094:Collagen / Q000235:genetics / Q000378:metabolism; D005109:Extracellular Matrix / Q000378:metabolism; D005333:Fetus; D006801:Humans; D008297:Male; D009369:Neoplasms / Q000378:metabolism*; D011371:Progeria / Q000378:metabolism; D008249:Protein-Lysine 6-Oxidase / Q000235:genetics / Q000378:metabolism*; D012333:RNA, Messenger
D000199:Actins; D012333:RNA, Messenger; D003094:Collagen; D008249:Protein-Lysine 6-Oxidase