Retrovirus vectors displaying the IgG-binding domain of protein A.
We have designed and constructed retrovirus particles displaying the IgG-binding domain of protein A. We fused the gene for the synthetic antibody-binding portion of protein A with the envelope gene of ecotropic Moloney murine leukemia virus. The fusion gene was coexpressed in ecotropic retroviral packaging cells, and retrovirus particles with IgG-binding activities were recovered. In principle, the protein A-envelope chimeric retrovirus complexed with specific monoclonal antibody could be used for cell-targeted gene delivery.
Ohno
K
K
Kaplan Cancer Center, New York University Medical Center, 560 First Avenue, New York, New York 10016, USA.
Meruelo
D
D
D000818:Animals; D000937:Antigen-Antibody Reactions; D001665:Binding Sites; D019556:COS Cells; D002352:Carrier Proteins / Q000235:genetics*; D005822:Genetic Vectors; D007074:Immunoglobulin G / Q000378:metabolism*; D010957:Plasmids; D012190:Retroviridae / Q000235:genetics*; D013205:Staphylococcal Protein A / Q000378:metabolism*
D002352:Carrier Proteins; D007074:Immunoglobulin G; D013205:Staphylococcal Protein A