title: The DnaJ60 gene of Drosophila melanogaster encodes a new member of the DnaJ family of proteins.
abstract: In eukaryotes the DnaJ homolog constitute a family of proteins with diverse functions which all appear to involve the chaperone activity of Hsp70. Here, we report the molecular characterization of DnaJ60, a gene located at 60C on the right arm of the second chromosome of Drosophila melanogaster and encoding a putative protein of 217 amino acids with a molecular mass of 27.7-kDa and a pl of 10.5. The N-terminal region of the DnaJ60 protein displays a significant sequence similarity with the J domain of DnaJ proteins and contains a centrally located hydrophobic segment suggesting the occurrence of a membrane spanning domain. Northern blot analysis detected a 0.75-kb transcript which is weakly expressed in embryos, larvae and females but intensively expressed in adult males. In situ localization revealed that the DnaJ60 transcript is highly expressed in male testes and the ejaculary bulb but at an undetectable level in ovaries suggesting that the DnaJ60 protein may play an important function during spermatogenesis and/or in the male genital tract.
authors.affiliation: Department of Developmental Genetics 0125, Deutsches Krebsforschungszentrum, Heidelberg, Germany.
authors.forename: I
authors.initials: I
authors.lastname: Iliopoulos
authors.forename: I
authors.initials: I
authors.lastname: Török
authors.forename: B M
authors.initials: BM
authors.lastname: Mechler
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D004330:Drosophila / Q000196:embryology / Q000235:genetics*; D005260:Female; D005786:Gene Expression Regulation / Q000235:genetics*; D050956:HSP40 Heat-Shock Proteins; D006360:Heat-Shock Proteins / Q000235:genetics*; D019476:Insect Proteins / Q000737:chemistry / Q000235:genetics*; D007814:Larva / Q000235:genetics; D008297:Male; D008969:Molecular Sequence Data; D008970:Molecular Weight; D017386:Sequence Homology, Amino Acid; D012737:Sex Factors
chemical_list: D050956:HSP40 Heat-Shock Proteins; D006360:Heat-Shock Proteins; D019476:Insect Proteins