Glutamate excitation of oxytocin neurones in vitro involves predominantly non-NMDA receptors.
Experiments were undertaken to compare effects of the NMDA and non-NMDA receptor antagonists, AP5 (40 microM) and NBQX (10 microM), on glutamate-induced firing in supraoptic oxytocin (OT) and vasopressin (VP) neurones in vitro. In putative OT neurones NBQX caused a significantly greater reduction in firing than AP5, whilst in putative VP neurones both antagonists reduced activity powerfully and to a similar extent. The relatively small effect of AP5 in putative OT neurones was unaffected by the removal of extracellular magnesium. These results suggest that glutamate-induced firing in putative OT neurones is predominantly controlled by non-NMDA receptors.
Richardson
C M
CM
Department of Anatomy, School of Medical Sciences, University of Bristol, UK.
Wakerley
J B
JB
D015763:2-Amino-5-phosphonovalerate / Q000494:pharmacology; D000818:Animals; D016308:Enkephalin, Leucine-2-Alanine / Q000494:pharmacology; D018691:Excitatory Amino Acid Antagonists / Q000494:pharmacology*; D005260:Female; D018698:Glutamic Acid / Q000494:pharmacology*; D066298:In Vitro Techniques; D009474:Neurons / Q000737:chemistry / Q000187:drug effects*; D010121:Oxytocin / Q000032:analysis*; D011810:Quinoxalines / Q000494:pharmacology; D051381:Rats; D017208:Rats, Wistar
D018691:Excitatory Amino Acid Antagonists; D011810:Quinoxalines; C062865:2,3-dioxo-6-nitro-7-sulfamoylbenzo(f)quinoxaline; D018698:Glutamic Acid; D010121:Oxytocin; D016308:Enkephalin, Leucine-2-Alanine; D015763:2-Amino-5-phosphonovalerate