title: Identification and functional assay of an extracellular calcium-sensing receptor in Necturus gastric mucosa.
abstract: In mammals and amphibians, increases in extracellular Ca2+ can activate bicarbonate secretion and other protective functions of gastric mucosa. We hypothesized that the recently cloned extracellular Ca(2+)-sensing receptor (CaR) is functioning in the gastric mucosa. In Necturus maculosus gastric mucosa, reverse transcription-polymerase chain reaction using primers based on previously cloned CaR sequences amplified a 326-bp DNA fragment that had 84% nucleotide sequence identity with the rat kidney CaR. Immunohistochemical localization of the CaR using specific anti-CaR antiserum revealed its presence on the basal aspect of gastric epithelial cells. In microelectrode studies of Necturus antral mucosa, exposure to elevated Ca2+ (4.8 mM) and the CaR agonists NPS-467 and neomycin sulfate resulted in significant hyperpolarizations of basal membrane electrical potentials and increases in apical-to-basal membrane resistance ratios. Circuit analysis revealed that these changes reflected specific decreases in basolateral membrane resistance. Inhibition of prostaglandin synthesis using indomethacin significantly attenuated these effects. We conclude that the CaR is present and functioning in Necturus gastric antrum.
authors.affiliation: Department of Surgery, West Roxbury Veterans Affairs Medical Center, Massachusetts, USA.
authors.forename: R R
authors.initials: RR
authors.lastname: Cima
authors.forename: I
authors.initials: I
authors.lastname: Cheng
authors.forename: M E
authors.initials: ME
authors.lastname: Klingensmith
authors.forename: N
authors.initials: N
authors.lastname: Chattopadhyay
authors.forename: O
authors.initials: O
authors.lastname: Kifor
authors.forename: S C
authors.initials: SC
authors.lastname: Hebert
authors.forename: E M
authors.initials: EM
authors.lastname: Brown
authors.forename: D I
authors.initials: DI
authors.lastname: Soybel
mesh_terms: D000584:Amiloride / Q000494:pharmacology; D000595:Amino Acid Sequence; D000818:Animals; D002462:Cell Membrane / Q000187:drug effects / Q000502:physiology; D003001:Cloning, Molecular; D005748:Gastric Fundus; D005753:Gastric Mucosa / Q000187:drug effects / Q000502:physiology*; D008564:Membrane Potentials / Q000187:drug effects; D008839:Microelectrodes; D008969:Molecular Sequence Data; D009337:Necturus; D009355:Neomycin / Q000494:pharmacology; D016133:Polymerase Chain Reaction; D011706:Pyloric Antrum; D051381:Rats; D044169:Receptors, Calcium-Sensing; D011956:Receptors, Cell Surface / Q000096:biosynthesis / Q000737:chemistry* / Q000502:physiology*; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid; D014158:Transcription, Genetic
chemical_list: D044169:Receptors, Calcium-Sensing; D011956:Receptors, Cell Surface; C473605:extracellular calcium cation-sensing receptor, rat; D000584:Amiloride; D009355:Neomycin