Evidence for a tungsten-stimulated aldehyde dehydrogenase activity of Desulfovibrio simplex that oxidizes aliphatic and aromatic aldehydes with flavins as coenzymes.
The aldehyde dehydrogenase activity of the sulfate-reducing bacterium Desulfovibrio simplex strain DSM 4141 was characterized in cell-free extracts. Oxygen-sensitive, constitutive aldehyde dehydrogenase activity was found in cells grown on l(+)-lactate, hydrogen, or vanillin with sulfate as the electron acceptor. A 1.83- to 2.6-fold higher specific activity was obtained in cells grown in media supplemented with 1 microM WO42-. The aldehyde dehydrogenase in cell-free extracts catalyzed the oxidation of aliphatic (Km < 20 microM) and aromatic aldehydes (Km < 0.32 mM) using methyl viologen as the electron acceptor. Flavins (FMN and FAD) were also active and are proposed to be the natural cofactors, while no activity was obtained with NAD+ or NADP+. 185WO42- was incorporated in vivo into D. simplex; it was found exclusively in the soluble fraction (>/= 98%). Anionic-exchange chromatography demonstrated coelution of 185W with two distinct peaks, the first one containing hydrogenase and formate dehydrogenase activities, and the second one aldehyde dehydrogenase activity.
Zellner
G
G
Institut für Mikrobiologie, Universität Hannover, Germany. gerhard.zellner@algemeen.micr.wau.nl
Jargon
A
A
D000444:Aldehyde Dehydrogenase / Q000187:drug effects* / Q000378:metabolism*; D000447:Aldehydes / Q000378:metabolism*; D003901:Desulfovibrio / Q000201:enzymology* / Q000254:growth & development / Q000378:metabolism; D004789:Enzyme Activation / Q000187:drug effects; D005486:Flavin Mononucleotide / Q000378:metabolism*; D005182:Flavin-Adenine Dinucleotide / Q000378:metabolism*; D010084:Oxidation-Reduction; D011868:Radioisotopes / Q000378:metabolism; D013347:Subcellular Fractions / Q000201:enzymology; D014414:Tungsten / Q000494:pharmacology*; D017973:Tungsten Compounds / Q000378:metabolism
D000447:Aldehydes; D011868:Radioisotopes; D017973:Tungsten Compounds; D005182:Flavin-Adenine Dinucleotide; D005486:Flavin Mononucleotide; D000444:Aldehyde Dehydrogenase; C045951:tungstate; D014414:Tungsten