Scanning slit confocal microscopy of fungal keratitis.
In vivo scanning slit confocal microscopy was performed in a patient with Fusarium solani keratitis. Morphologically distinctive abundant filamentous structures were observed intrastromally. Confocal microscopy of the culture plate growing F solani from the patient's corneal scraping revealed filaments morphologically similar to the filaments observed in vivo. After 1 week of medical therapy, subsequent confocal microscopy showed an increased load of filaments, supporting the decision to perform a penetrating keratoplasty. Confocal microscopy confirmed that all of the fungus was eradicated. This aided in the decision to administer corticosteroids and quickly discontinue antifungal agents.
Florakis
G J
GJ
Edward S. Harkness Eye Institute, Columbia University, New York, NY, USA.
Moazami
G
G
Schubert
H
H
Koester
C J
CJ
Auran
J D
JD
D000293:Adolescent; D000935:Antifungal Agents / Q000627:therapeutic use; D003319:Corneal Stroma / Q000382:microbiology / Q000473:pathology; D003320:Corneal Ulcer / Q000175:diagnosis* / Q000382:microbiology / Q000628:therapy; D015821:Eye Infections, Fungal / Q000175:diagnosis* / Q000209:etiology / Q000628:therapy; D005670:Fusarium / Q000302:isolation & purification*; D006801:Humans; D015948:Keratoplasty, Penetrating; D008297:Male; D018613:Microscopy, Confocal / Q000379:methods*; D009181:Mycoses / Q000175:diagnosis* / Q000209:etiology / Q000628:therapy
D000935:Antifungal Agents