Structure determination of N-linked oligosaccharides engineered at the CH1 domain of humanized LL2.
Two humanized antibody mutants, hLL2HCN1 and hLL2HCN5, engineered with CH1 domain-appended carbohydrates (CHOs) were generated to facilitate site-specific conjugation of radionuclides and anti-cancer drugs to antibodies. Such site-specific conjugation may minimize the incidence of immunoreactivity perturbation as is often observed with random conjugation. Since the compositions and structures of CHOs are important in determining the chemistry, efficiency, and extent of conjugation, the sequences of the CH1-appended CHOs were determined by exoglycosidase digestions and fluorophore-assisted CHO electrophoresis (FACE). The CHO species attached at HCN1 and HCN5 sites in hLL2HCN1 and hLL2HCN5, respectively, were distinct from each other, heterogeneous, and extensively processed. All of these CHOs were core-fucosylated complex-type oligosaccharides and contained Gal (galactose) and GlcNAc (N-acetylglucosamine) residues in the outer branches. Some of the outer branches were composed of Gal alpha1-3Galbeta1-4GlcNAc structure, also known as alpha-galactosyl epitope. Most of the CHOs were sialylated. While all HCN1-CHOs were biantennary, the majority of HCN5-CHOs (>60%) were triantennary. The CH1-appended CHOs have favorable structural characteristics suitable for site-specific conjugation. For efficient conjugation of large drug complexes, hLL2HCN5 is preferable to hLL2HCN1 because the attached CHO is larger in size and more remotely positioned from the V region. The effects of the alpha-galactosyl epitope found in these CHOs on the immunological properties of the immunoconjugates as efficient cancer diagnostics and therapeutics are being studied.
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Z
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Immunomedics, Inc., Morris Plains, NJ 07950, USA.
Sharkey
R M
RM
Hansen
H J
HJ
Goldenberg
D M
DM
Leung
S
S
D000911:Antibodies, Monoclonal / Q000737:chemistry*; D002236:Carbohydrate Conformation; D000939:Epitopes / Q000737:chemistry; D005690:Galactose / Q000737:chemistry; D006031:Glycosylation; D006801:Humans; D009844:Oligosaccharides / Q000737:chemistry*; D015202:Protein Engineering
D000911:Antibodies, Monoclonal; D000939:Epitopes; D009844:Oligosaccharides; D005690:Galactose