title: Novel butyrolactones with antifungal activity produced by Pseudomonas aureofaciens strain 63-28.
abstract: The bacterium Pseudomonas aureofaciens 63-28 is antagonistic to several plant pathogenic fungi, including Pythium spp. The bacterium produced at least four antifungal metabolites active against Pythium ultimum and Phytophthora cryptogea when tested in culture for antifungal activity. Two of these compounds were identified as the novel butyrolactones (Z)-4-hydroxy-4-methyl-2-(1-hexenyl)-2-butenolide and (Z)-4-hydroxymethyl-2-(1-hexenyl)-2-butenolide, by using NMR and GC-MS. All compounds were different from other antibiotics produced by Pseudomonas spp., including pyoluteorin, pyrrolnitrin, and 2,4-diacetylphloroglucinol, as determined by HPLC. This is the first report of butyrolactones with antifungal activity produced by a saprophytic Pseudomonas spp.
authors.affiliation: Department of Plant Science, McGill University, QC, Canada.
authors.forename: P
authors.initials: P
authors.lastname: Gamard
authors.forename: F
authors.initials: F
authors.lastname: Sauriol
authors.forename: N
authors.initials: N
authors.lastname: Benhamou
authors.forename: R R
authors.initials: RR
authors.lastname: Bélanger
authors.forename: T C
authors.initials: TC
authors.lastname: Paulitz
mesh_terms: D015107:4-Butyrolactone / Q000031:analogs & derivatives / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D000935:Antifungal Agents / Q000737:chemistry* / Q000302:isolation & purification* / Q000494:pharmacology; D002851:Chromatography, High Pressure Liquid; D009682:Magnetic Resonance Spectroscopy; D008826:Microbial Sensitivity Tests; D011549:Pseudomonas; D013329:Structure-Activity Relationship
chemical_list: C109006:4-hydroxy-4-methyl-2-(1-hexenyl)-2-butenolide; C109007:4-hydroxymethyl-2-(1-hexenyl)-2-butenolide; D000935:Antifungal Agents; D015107:4-Butyrolactone