Maternal control of a zygotic patterning gene in Caenorhabditis elegans.
The transition from maternal to zygotic gene control is a key process in embryogenesis. Although many maternal effect genes have been studied in the C. elegans embryo, how their activities lead to the positional expression of zygotic patterning genes has not yet been established. Evidence is presented showing that expression of the zygotic patterning gene vab-7 does not depend on cell position or cell contacts, but rather on the production of a C blastomere. Furthermore, pal-1, a caudal homologue with maternal product necessary for the proper development of the C blastomere, is both necessary and sufficient for vab-7 expression. This provides a link between maternal gene activity and zygotic patterning gene expression in C. elegans. The results suggest that zygotic patterning genes might be generally controlled at the level of blastomere fate and not by position.
Ahringer
J
J
University of Cambridge, Department of Genetics, UK. jaa@gen.cam.ac.uk
D000818:Animals; D001757:Blastomeres / Q000502:physiology; D019521:Body Patterning / Q000235:genetics*; D017173:Caenorhabditis elegans / Q000196:embryology* / Q000235:genetics*; D029742:Caenorhabditis elegans Proteins; D004268:DNA-Binding Proteins; D004625:Embryo, Nonmammalian / Q000502:physiology*; D005260:Female; D018507:Gene Expression Regulation, Developmental; D017238:Genes, Helminth; D018392:Genomic Imprinting; D015801:Helminth Proteins / Q000096:biosynthesis*; D018398:Homeodomain Proteins / Q000096:biosynthesis*; D008297:Male; D016133:Polymerase Chain Reaction; D016601:RNA-Binding Proteins / Q000096:biosynthesis; D011994:Recombinant Proteins / Q000096:biosynthesis; D015534:Trans-Activators; D014157:Transcription Factors; D015053:Zygote / Q000166:cytology / Q000502:physiology; D001616:beta-Galactosidase / Q000096:biosynthesis
D029742:Caenorhabditis elegans Proteins; D004268:DNA-Binding Proteins; D015801:Helminth Proteins; D018398:Homeodomain Proteins; C102175:MEX-3 protein, C elegans; D016601:RNA-Binding Proteins; D011994:Recombinant Proteins; D015534:Trans-Activators; D014157:Transcription Factors; C099772:VAB-7 protein, C elegans; C068665:pal-1 protein, C elegans; C073702:skn-1 protein, C elegans; D001616:beta-Galactosidase