A novel method to isolate cells with conditional gene expression using fluorescence activated cell sorting.
An inducible expression system using control elements of the tetracycline resistance operon has recently shown promise for conditional gene expression of any gene of interest. However, intensive screening of multiple independent clones is often required to find cell lines with optimal induction characteristics. By coupling expression of the gene of interest with a fluorescent marker, we have developed a novel fluorescence activated cell sorting (FACS) based strategy to isolate cells with desirable expression characteristics, thus alleviating the laborious isolation and analysis of multiple independent clones.
Klucher
K M
KM
Whitehead Institute for Biomedical Research, 9 Cambridge Center, Cambridge, MA 02142, USA.
Gerlach
M J
MJ
Daley
G Q
GQ
D000818:Animals; D002469:Cell Separation / Q000379:methods*; D005434:Flow Cytometry / Q000379:methods*; D016044:Fusion Proteins, bcr-abl / Q000235:genetics*; D005786:Gene Expression Regulation; D006801:Humans; D051379:Mice; D009876:Operon; D013753:Tetracycline Resistance / Q000235:genetics; D014407:Tumor Cells, Cultured
D016044:Fusion Proteins, bcr-abl