The effects of TYB-2285 and its metabolites on lymphocyte responses in vitro.
1. We examined the effects of TYB-2285 and its metabolites (TC-286 and TC-326) on antigen-induced lymphocyte proliferation, allogeneic mixed lymphocyte reaction (MLR) and mitogen-induced lymphocyte proliferation. 2. Splenic lymphocytes from C57BL/6 strain mice sensitized with human serum albumin were cultured with the antigen in the presence of TYB-2285 or its metabolites (10(-7)-10(-4) M). The lymphocyte proliferation enhanced by antigen was inhibited by TYB-2285 and its metabolites in a dose-dependent manner. 3. Splenic lymphocytes collected from C57BL/6 and Balb/C strain mice were cocultured in the presence of TYB-2285 and its metabolites (10(-7)-10(-4) M). Allogeneic MLR was inhibited by TYB-2285 and its metabolites in a dose-dependent manner. 4. Splenic lymphocytes from C47BL/6 strain mice were cultured with concanavalin A (Con A) in the presence of TYB-2285 and its metabolites (10(-7)-10(-4) M). TYB-2285 and its metabolites did not affect Con A-induced lymphocyte proliferation. Cyclosporin A showed an inhibitory effect on Con A-induced lymphocyte proliferation that was as strong as the inhibitory effect on antigen-induced lymphocyte proliferation and allogeneic MLR at a lower concentration.
Tominaga
T
T
Department of Pharmacology, Toyobo Co. Ltd., Ohtsu Shiga, Japan.
Watanabe
A
A
Tsuji
J
J
Koda
A
A
Nagai
H
H
Kumazawa
Y
Y
Shimada
H
H
D000418:Albumins / Q000276:immunology; D000818:Animals; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000494:pharmacology*; D000941:Antigens / Q000276:immunology; D002455:Cell Division / Q000187:drug effects; D003208:Concanavalin A / Q000494:pharmacology; D016572:Cyclosporine / Q000494:pharmacology; D066298:In Vitro Techniques; D007202:Indicators and Reagents; D008214:Lymphocytes / Q000187:drug effects* / Q000276:immunology; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D009570:Nitriles / Q000494:pharmacology*
D000418:Albumins; D000894:Anti-Inflammatory Agents, Non-Steroidal; D000941:Antigens; D007202:Indicators and Reagents; D009570:Nitriles; D003208:Concanavalin A; C081050:3,5-bis(acetoxyacetylamino)-4-chlorobenzonitrile; D016572:Cyclosporine