Quantification of furosemide from serum and tissues using high-performance liquid chromatography.
Since pharmacokinetics may play a significant role in furosemide (FSM) developmental ototoxicity, we developed an assay for the extraction and quantification of FSM in tissue and fluid from neonatal and adult rats. Rats from post-natal day (PND) 10, 30 and 50, were given an intravenous dose of FSM (35 mg/kg). Blood and tissues were analyzed by HPLC. FSM in serum, perilymph and liver was elevated in PND ten rats as was the body burden of FSM. Renal concentrations were higher in older rats. Altered clearance of FSM in developing rats may result in higher concentrations in the cochlea and ototoxicity.
Mills
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Amgen Inc., Thousand Oaks, CA 91320-1789, USA.
Whitworth
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Rybak
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Henley
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D000818:Animals; D000831:Animals, Newborn; D002851:Chromatography, High Pressure Liquid; D004232:Diuretics / Q000032:analysis / Q000097:blood* / Q000493:pharmacokinetics; D005665:Furosemide / Q000032:analysis / Q000097:blood* / Q000493:pharmacokinetics; D007668:Kidney / Q000737:chemistry*; D008099:Liver / Q000737:chemistry*; D010498:Perilymph / Q000737:chemistry*; D051381:Rats; D017207:Rats, Sprague-Dawley; D012680:Sensitivity and Specificity; D014018:Tissue Distribution
D004232:Diuretics; D005665:Furosemide