title: The trans-sialidase of Trypanosoma cruzi is anchored by two different lipids.
abstract: The trans-sialidase from the trypomastigote stage of Trypanosoma cruzi was metabolically labeled with [3H]-palmitic acid and purified by immunoprecipitation with a monoclonal antibody. The action of PI-PLC on the immunoprecipitate released a lipid that was analyzed by TLC. Lyso-1-O-hexadecylglycerol and N-palmitoyl-sphinganine were obtained in a 1:3 ratio. A comparison with the GPI anchors present in the different stages of T. cruzi was made.
authors.affiliation: CIHIDECAR, Departmento de Química Orgánica, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Argentina.
authors.forename: R
authors.initials: R
authors.lastname: Agusti
authors.forename: A S
authors.initials: AS
authors.lastname: Couto
authors.forename: O E
authors.initials: OE
authors.lastname: Campetella
authors.forename: A C
authors.initials: AC
authors.lastname: Frasch
authors.forename: R M
authors.initials: RM
authors.lastname: de Lederkremer
mesh_terms: D000818:Animals; D002855:Chromatography, Thin Layer; D017261:Glycosylphosphatidylinositols / Q000378:metabolism*; D008563:Membrane Lipids / Q000378:metabolism*; D051379:Mice; D009439:Neuraminidase / Q000378:metabolism*; D043265:Phosphatidylinositol Diacylglycerol-Lyase; D054801:Phosphoinositide Phospholipase C; D013379:Substrate Specificity; D014349:Trypanosoma cruzi / Q000201:enzymology*; D010738:Type C Phospholipases / Q000378:metabolism
chemical_list: D017261:Glycosylphosphatidylinositols; D008563:Membrane Lipids; D010738:Type C Phospholipases; D054801:Phosphoinositide Phospholipase C; D009439:Neuraminidase; D043265:Phosphatidylinositol Diacylglycerol-Lyase