Organization and expression of the chum salmon insulin-like growth factor II gene.
IGF-II plays an important role in growth and development of vertebrates. In the present study, the characterization of the first fish IGF-II gene, chum salmon IGF-II, is described. The sIGF-II gene consists of four exons, spanning a region of 9 kbp, that encode the 214 aa IGF-II precursor. While the amino acid sequences of fully processed IGF-II of salmon and mammalian species are very similar, the prepro-peptide sequence deviates extensively in the signal- and E-peptide domains. The transcription initiation site of the sIGF-II gene was localized within a 30 nt region employing RT-PCR. Using sIGF-II promoter-luciferase constructs it was demonstrated that the sIGF-II gene has a relatively strong promoter that contains tissue-specific regulatory elements.
Palamarchuk
A Y
AY
Department of Biosynthesis of Nucleic Acids, Institute of Molecular Biology and Genetics, National Academy of Sciences of the Ukraine, Kiev.
Holthuizen
P E
PE
Müller
W E
WE
Sussenbach
J S
JS
Kavsan
V M
VM
D000818:Animals; D001483:Base Sequence; D017931:DNA Primers; D005091:Exons; D005786:Gene Expression Regulation; D006801:Humans; D007335:Insulin-Like Growth Factor II / Q000096:biosynthesis* / Q000235:genetics*; D008969:Molecular Sequence Data; D017685:Oncorhynchus keta / Q000235:genetics*; D017686:Oncorhynchus mykiss; D016133:Polymerase Chain Reaction; D011401:Promoter Regions, Genetic; D011498:Protein Precursors / Q000096:biosynthesis / Q000235:genetics; D011994:Recombinant Proteins / Q000096:biosynthesis; D014162:Transfection; D014407:Tumor Cells, Cultured
D017931:DNA Primers; D011498:Protein Precursors; D011994:Recombinant Proteins; C066038:proinsulin-like growth factor II; D007335:Insulin-Like Growth Factor II