title: DNA-probes for the differentiation of Capnocytophaga species.
abstract: We designed oligonucleotides to differentiate between the seven currently known Capnocytophaga species. The oligonucleotides were labelled non-radioactively at the 3' end with digoxigenin. The specificity could be demonstrated in a dot-blot hybridization assay by using the type strains, reference strains, and 37 clinical Capnocytophaga isolates as well as 11 representative strains of other taxa as a template. The sensitivity of the assay was calculated with 10(3) bacteria per dot.
authors.affiliation: Clinic of Conservative & Preventive Dentstry and Periodontology, University Hospital (RWTH) Aachen, Germany.
authors.forename: G
authors.initials: G
authors.lastname: Conrads
authors.forename: R
authors.initials: R
authors.lastname: Mutters
authors.forename: I
authors.initials: I
authors.lastname: Seyfarth
authors.forename: K
authors.initials: K
authors.lastname: Pelz
mesh_terms: D015373:Bacterial Typing Techniques; D002206:Capnocytophaga / Q000145:classification* / Q000235:genetics; D015342:DNA Probes; D004269:DNA, Bacterial / Q000235:genetics; D004275:DNA, Ribosomal / Q000235:genetics; D004076:Digoxigenin; D009693:Nucleic Acid Hybridization; D009838:Oligodeoxyribonucleotides / Q000737:chemistry; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012680:Sensitivity and Specificity; D013045:Species Specificity
chemical_list: D015342:DNA Probes; D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D009838:Oligodeoxyribonucleotides; D012336:RNA, Ribosomal, 16S; D004076:Digoxigenin