title: Recombinant human transforming growth factor beta 3 accelerates gastric ulcer healing in rats.
abstract: BACKGROUND
  Gastric ulcer healing is mediated by various endogenous growth factors. In this experimental study effect of locally and systemically applied recombinant human transforming growth factor beta 3 (rhTGF-beta 3) on gastric ulcer healing was investigated in the rat.
  
  
  RESULTS
  Gastric ulcers were induced with a cryoprobe, and ulcer healing was evaluated 7 days after local infiltration (0.5 micrograms, 1.0 microgram, 2.5 micrograms, and 50 micrograms) or systemic (intravenous) application of TGF-beta 3 (500 micrograms/kg body weight). Compared with controls, a dose-dependent stimulation of ulcer healing (as evidenced by a reduction in ulcer size) was observed 7 days after local infiltration of TGF-beta 3 (1.0 microgram, 2.5 micrograms, and 50 micrograms). Corresponding increases in the levels of proliferating cell nuclear antigen (PCNA) and intracellular TGF-beta 3 expression and a downregulation of the TGF-beta type-II receptor expression were also observed in the granulation tissue of the ulcer margins. Systemic application of TGF-beta 3 produced effects similar to those observed after local treatment with 50 micrograms of the compound.
  
  
  CONCLUSIONS
  Local and systemic TGF-beta 3 treatment accelerates gastric ulcer healing in rats.
authors.affiliation: Dept. of General and Transplant Surgery, University of Tübingen Germany.
authors.forename: S
authors.initials: S
authors.lastname: Coerper
authors.forename: E
authors.initials: E
authors.lastname: Sigloch
authors.forename: D
authors.initials: D
authors.lastname: Cox
authors.forename: M
authors.initials: M
authors.lastname: Starlinger
authors.forename: G
authors.initials: G
authors.lastname: Köveker
authors.forename: H D
authors.initials: HD
authors.lastname: Becker
mesh_terms: D000818:Animals; D004305:Dose-Response Relationship, Drug; D005260:Female; D006801:Humans; D051381:Rats; D017208:Rats, Wistar; D011994:Recombinant Proteins / Q000627:therapeutic use; D013268:Stimulation, Chemical; D013276:Stomach Ulcer / Q000188:drug therapy*; D013997:Time Factors; D016212:Transforming Growth Factor beta / Q000627:therapeutic use*; D014945:Wound Healing / Q000187:drug effects*
chemical_list: D011994:Recombinant Proteins; D016212:Transforming Growth Factor beta