title: Aneuploidy induction by benzo[a]pyrene and polyploidy induction by 7,12-dimethylbenz[a]anthracene in Chinese hamster cell lines V79-MZ and V79.
abstract: We found that different ploidy effects were induced in four Chinese hamster-derived cell lines (V79-MZ, V79, CHL and CHO-K1) treated through two cell cycles with polycyclic aromatic hydrocarbons in the absence of a metabolic activation system. 5-Bromodeoxyuridine was used to investigate cell cycle delay and sister chromatid exchanges (SCE) induced by the chemicals. Benzo[a]pyrene (BP) induced aneuploidy at 2.5-10 micrograms/ml in V79-MZ cells. 7,12-Dimethylbenz[a]anthracene (DMBA) induced polyploidy at 3.125-6.25 and 6.25-1.25 micrograms/ml in V79-MZ and V79 cells respectively. Higher concentrations caused cell cycle delay and, therefore, did not affect ploidy. BP and DMBA did not induce a significant increase in SCE frequency at the above doses. 3-Methylcholanthrene tested up to its solubility limit (10 micrograms/ml) did not induce numerical aberrations in any cell line. The clastogen mitomycin C, tested up to 0.01 microgram/ml, did not produce numerical aberrations but did significantly increase SCE frequency in all cell lines. The spindle poison colchicine, tested up to 0.1 microgram/ml, induced ploidy changes in the four cell lines that showed different sensitivities. Four cell lines showed no arylhydrocarbon hydroxylase activity, and V79-MZ, but not the other cells lines, showed high glutathione S-transferase activity. Aneuploidy induction by BP and polyploidy induction by DMBA in the absence of S9 mix in vitro have not been described before, and the finding might be due to the effect on tubulin. Due to their specificity and high sensitivity, the V79-MZ and V79 cell lines might be good systems for detecting aneuploidogens.
authors.affiliation: Division of Genetics and Mutagenesis, National Institute of Health Sciences, Tokyo, Japan.
authors.forename: A
authors.initials: A
authors.lastname: Matsuoka
authors.forename: M
authors.initials: M
authors.lastname: Ozaki
authors.forename: K
authors.initials: K
authors.lastname: Takeshita
authors.forename: H
authors.initials: H
authors.lastname: Sakamoto
authors.forename: H R
authors.initials: HR
authors.lastname: Glatt
authors.forename: M
authors.initials: M
authors.lastname: Hayashi
authors.forename: T
authors.initials: T
authors.lastname: Sofuni
mesh_terms: D015127:9,10-Dimethyl-1,2-benzanthracene / Q000633:toxicity*; D000782:Aneuploidy; D000818:Animals; D001564:Benzo(a)pyrene / Q000633:toxicity*; D016466:CHO Cells; D002453:Cell Cycle / Q000187:drug effects; D002460:Cell Line; D002869:Chromosome Aberrations; D003078:Colchicine / Q000633:toxicity; D006224:Cricetinae; D003412:Cricetulus; D008748:Methylcholanthrene / Q000633:toxicity; D016685:Mitomycin / Q000633:toxicity; D009153:Mutagens / Q000633:toxicity*; D011123:Polyploidy; D012854:Sister Chromatid Exchange / Q000187:drug effects*
chemical_list: D009153:Mutagens; D001564:Benzo(a)pyrene; D016685:Mitomycin; D008748:Methylcholanthrene; D015127:9,10-Dimethyl-1,2-benzanthracene; D003078:Colchicine