Dietary NaCl and KCl do not regulate renal density of the thiazide diuretic receptor.
We tested the postulate that the renal density of the thiazide-inhibitable Na-Cl cotransporter or thiazide receptor (TZR) is modulated as part of the renal homeostatic response to changes in dietary intake of NaCl or KCl. Renal excretion of NaCl or KCl varied > 10-fold in response to alterations in oral intake. Renal TZR density was quantitated by binding of [3H]metolazone to renal membranes. Renal TZR density was not altered by sodium deficit (with increased plasma aldosterone concentration), by sodium surfeit (8% NaCl content of diet), by potassium deficit (with hypokalemia), or by potassium surfeit (drinking 1% KCl solution). Unexpectedly, we conclude that regulation of the renal density of TZR is not part of the renal homeostatic responses that adjust excretion of NaCl and KCl to changes in dietary intake of NaCl or KCl.
Fanestil
D D
DD
Department of Medicine, University of California San Diego, La Jolla 92093-0623, USA.
Vaughn
D A
DA
Blakely
P
P
D000450:Aldosterone / Q000097:blood; D000818:Animals; D002352:Carrier Proteins / Q000187:drug effects* / Q000378:metabolism*; D002712:Chlorides / Q000652:urine; D004032:Diet; D007668:Kidney / Q000378:metabolism*; D008297:Male; D009318:Natriuresis / Q000502:physiology; D011188:Potassium / Q000652:urine; D011189:Potassium Chloride / Q000008:administration & dosage* / Q000494:pharmacology; D051381:Rats; D017207:Rats, Sprague-Dawley; D011955:Receptors, Drug / Q000187:drug effects* / Q000378:metabolism*; D012965:Sodium Chloride / Q000008:administration & dosage* / Q000494:pharmacology; D049992:Sodium Chloride Symporters; D064486:Solute Carrier Family 12, Member 3; D027981:Symporters
D002352:Carrier Proteins; D002712:Chlorides; D011955:Receptors, Drug; C578876:Slc12a3 protein, rat; D049992:Sodium Chloride Symporters; D064486:Solute Carrier Family 12, Member 3; D027981:Symporters; C064415:thiazide receptor; D012965:Sodium Chloride; D000450:Aldosterone; D011189:Potassium Chloride; D011188:Potassium