Identification of Listeria monocytogenes by colony hybridization test using the virulence-associated hly and inlA genes as probes.
We developed a method of identification of Listeria monocytogenes based on colony hybridization with nonradioactively labeled DNA probes, represented by the hly and inlA virulence-associated genes. The procedure described in this paper results simple, rapid, specific and reproducible. Since it can be performed in a short time, the above technique can be applied to detect L. monocytogenes from different source and constitutes a noteworthy and alternative tool to identify this gram-positive pathogenic bacterium.
Petrone
G
G
Ist. di Microbiologia, Facoltà di Medicina e Chirurgia, Università degli Studi di Roma La Sapienza.
Polidoro
M
M
Donnarumma
G
G
Conte
M P
MP
Papi
E
E
Seganti
L
L
Valenti
P
P
D015373:Bacterial Typing Techniques; D015342:DNA Probes / Q000235:genetics*; D005516:Food Microbiology; D005798:Genes, Bacterial / Q000235:genetics*; D008089:Listeria monocytogenes / Q000145:classification* / Q000235:genetics / Q000472:pathogenicity; D014774:Virulence / Q000235:genetics
D015342:DNA Probes