Involvement of protein kinase C in the activation of extracellular signal-regulated kinase 1/2 by UVC irradiation.
UVC irradiation activates mitogen-activated protein kinases (MAPKs), including ERK, JNK, and P38. This study examined the role of protein kinase C (PKC) in the regulation of UVC-stimulated MAPKs activation. Either the depletion of PKC by prolonged treatment of cells with 12-O-tetradecanoylphorbol-13-acetate (TPA) or the inhibition of PKC by a selective PKC inhibitor, UCN-01-ME, attenuated UVC-activation of ERK1/2, keeping the activation of JNK1/2 intact. However, K252a, a non-selective PKC inhibitor, inhibited the activation of both ERK1/2 and JNK1/2 by UVC. In three isoforms of PKC (alpha, delta, epsilon) examined, PKC epsilon shows the most evident translocation, a temporal association with cell membrane, upon the UVC irradiation of NIH 3T3 cells. These results suggest that PKC is acting in the UVC-dependent activation of ERK1/2, and PKC epsilon is one of the PKC isozymes playing such a role.
Zhuang
S
S
Department of Molecular Biology, Yokohama City University School of Medicine, Japan.
Hirai
S
S
Mizuno
K
K
Suzuki
A
A
Akimoto
K
K
Izumi
Y
Y
Yamashita
A
A
Ohno
S
S
D016475:3T3 Cells; D000818:Animals; D017871:Calcium-Calmodulin-Dependent Protein Kinases / Q000378:metabolism / Q000528:radiation effects*; D016895:Culture Media, Serum-Free; D004789:Enzyme Activation / Q000528:radiation effects; D006801:Humans; D007527:Isoenzymes / Q000096:biosynthesis / Q000378:metabolism / Q000528:radiation effects; D048031:JNK Mitogen-Activated Protein Kinases; D007700:Kinetics; D051379:Mice; D019950:Mitogen-Activated Protein Kinase 1; D048052:Mitogen-Activated Protein Kinase 3; D020928:Mitogen-Activated Protein Kinases; D011493:Protein Kinase C / Q000096:biosynthesis / Q000378:metabolism* / Q000528:radiation effects*; D051571:Protein Kinase C-alpha; D051745:Protein Kinase C-delta; D051744:Protein Kinase C-epsilon; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000378:metabolism / Q000528:radiation effects; D014162:Transfection; D014466:Ultraviolet Rays
D016895:Culture Media, Serum-Free; D007527:Isoenzymes; D011994:Recombinant Proteins; C497259:Prkcd protein, mouse; C497256:Prkce protein, mouse; C496628:PRKCA protein, human; C497258:PRKCD protein, human; C497255:PRKCE protein, human; C496630:Prkca protein, mouse; D011493:Protein Kinase C; D051571:Protein Kinase C-alpha; D051745:Protein Kinase C-delta; D051744:Protein Kinase C-epsilon; D017871:Calcium-Calmodulin-Dependent Protein Kinases; D048031:JNK Mitogen-Activated Protein Kinases; D019950:Mitogen-Activated Protein Kinase 1; D048052:Mitogen-Activated Protein Kinase 3; D020928:Mitogen-Activated Protein Kinases