Streptomyces lividans groES, groEL1 and groEL2 genes.
The Streptomyces lividans groES/EL1 operon and groEL2 gene were cloned and their respective DNA sequences determined. The sequenced DNA comprised the genes and their respective regulatory regions in both cases. Transcription of both groES/EL1 and groEL2 seemed to be subjected to temporal control at 30 degrees C. At 45 degrees C the amount of the groEL2 transcript increased considerably in comparison to that of groES/EL1. Among the proteins synthesized under heat shock by S. lividans, a fraction enriched in GroEL2 showed the presence of a ring-shaped structure that resembles that of other chaperonins and was active in a rhodanase folding assay.
de León
Patricia
P
Centro Nacional de Biotecnología (CSIC), Campus de la Universidad Autónoma, Cantoblanco, 28049 Madrid, Spain.
Marco
Sergio
S
Centro Nacional de Biotecnología (CSIC), Campus de la Universidad Autónoma, Cantoblanco, 28049 Madrid, Spain.
Isiegas
Carolina
C
Centro Nacional de Biotecnología (CSIC), Campus de la Universidad Autónoma, Cantoblanco, 28049 Madrid, Spain.
Marina
Anabel
A
Centra de Biología Molecular Severo Ochoa, Campus de la Universidad Autónoma, Cantoblanco, 28049 Madrid, Spain.
Carrascosa
José L
JL
Centro Nacional de Biotecnología (CSIC), Campus de la Universidad Autónoma, Cantoblanco, 28049 Madrid, Spain.
Mellado
Rafael P
RP
Centro Nacional de Biotecnología (CSIC), Campus de la Universidad Autónoma, Cantoblanco, 28049 Madrid, Spain.
D000595:Amino Acid Sequence; D001483:Base Sequence; D018835:Chaperonin 10 / Q000235:genetics*; D018834:Chaperonin 60 / Q000235:genetics* / Q000302:isolation & purification / Q000648:ultrastructure; D003001:Cloning, Molecular; D018628:Gene Dosage; D015964:Gene Expression Regulation, Bacterial / Q000235:genetics / Q000502:physiology; D005798:Genes, Bacterial / Q000235:genetics*; D018869:Heat-Shock Response / Q000235:genetics*; D008969:Molecular Sequence Data; D017510:Protein Folding; D012329:RNA, Bacterial / Q000096:biosynthesis; D012333:RNA, Messenger / Q000096:biosynthesis; D012045:Regulatory Sequences, Nucleic Acid / Q000235:genetics; D015183:Restriction Mapping; D017421:Sequence Analysis; D017422:Sequence Analysis, DNA; D013302:Streptomyces / Q000235:genetics*; D014158:Transcription, Genetic
D018835:Chaperonin 10; D018834:Chaperonin 60; D012329:RNA, Bacterial; D012333:RNA, Messenger