title: Immunohistochemical detection of CD5 with monoclonal antibody 4C7 on paraffin sections.
abstract: CD5 is useful for the classification of low-grade B-cell lymphomas, because it is aberrantly expressed in B-cell chronic lymphocytic leukemias/small lymphocytic lymphomas (B-CLLs/SLLs) and mantle cell lymphomas (MCLs), but not in the other well-described low-grade B-cell lymphomas. Therefore, we tested the utility of monoclonal antibody (MAb) 4C7 to detect CD5 on paraffin sections of 22 cases with low-grade B-cell lymphomas and 4 cases with high-grade B-cell lymphomas and compared the results with CD5 detection by analysis of fresh tissue with a fluorescent-activated cell sorting (FACS). After heat-induced epitope retrieval we could detect CD5 on paraffin sections in all MCLs and B-CLLs/SLLs, identical to the results of flow cytometry. Eleven of 12 cases that showed no CD5 expression by FACS analysis were also negative on paraffin sections. In only one case of a presumed blastoid MCL, CD5 was detectable by immunohistochemistry but not by FACS analysis. We conclude that MAb 4C7 against CD5 is a useful paraffin-reactive marker, especially to correctly identify MCLs on paraffin sections.
authors.affiliation: Institute of Pathology, Charité University Hospital, Berlin, Germany.
authors.forename: O
authors.initials: O
authors.lastname: Kaufmann
authors.forename: B
authors.initials: B
authors.lastname: Flath
authors.forename: E
authors.initials: E
authors.lastname: Späth-Schwalbe
authors.forename: K
authors.initials: K
authors.lastname: Possinger
authors.forename: M
authors.initials: M
authors.lastname: Dietel
mesh_terms: D000911:Antibodies, Monoclonal / Q000032:analysis*; D018956:CD5 Antigens / Q000032:analysis* / Q000276:immunology; D005069:Evaluation Studies as Topic; D005434:Flow Cytometry; D006801:Humans; D007150:Immunohistochemistry; D016393:Lymphoma, B-Cell / Q000145:classification* / Q000276:immunology*; D008228:Lymphoma, Non-Hodgkin / Q000145:classification / Q000276:immunology*; D016612:Paraffin Embedding
chemical_list: C109146:4C7 monoclonal antibody; D000911:Antibodies, Monoclonal; D018956:CD5 Antigens