title: Basic neural transplantation techniques. I. Dissociated cell suspension grafts of embryonic ventral mesencephalon in the adult rat brain.
abstract: Lesions and grafts in the nigrostriatal dopamine system in rats is widely used as a model of degeneration and regeneration in the CNS, and the development for alternative strategies for treatment in Parkinson's disease. The methods of preparing a dissociated cell suspension of embryonic rat substantia nigra and its transplantation into the brain of adult rats are described. This is the first of a series of methodological reports on the basic methodology and refinements of neural transplantation techniques in the mammalian central nervous system.
authors.affiliation: MRC Cambridge Centre for Brain Repair, University of Cambridge, UK. sd19@cam.ac.uk
authors.forename: S B
authors.initials: SB
authors.lastname: Dunnett
authors.forename: A
authors.initials: A
authors.lastname: Björklund
mesh_terms: D000818:Animals; D001921:Brain / Q000378:metabolism / Q000601:surgery*; D017690:Cell Transplantation / Q000379:methods*; D003342:Corpus Striatum / Q000378:metabolism / Q000601:surgery; D004298:Dopamine / Q000378:metabolism; D005260:Female; D016332:Fetal Tissue Transplantation / Q000379:methods*; D006085:Graft Survival; D008297:Male; D008636:Mesencephalon / Q000166:cytology / Q000196:embryology*; D009417:Nerve Tissue / Q000166:cytology / Q000196:embryology*; D009488:Neurosciences / Q000379:methods*; D051381:Rats / Q000196:embryology; D013378:Substantia Nigra / Q000166:cytology / Q000196:embryology; D014446:Tyrosine 3-Monooxygenase / Q000378:metabolism
chemical_list: D014446:Tyrosine 3-Monooxygenase; D004298:Dopamine