title: Eosinophilic leukocyte accumulation during vagally induced bronchoconstriction.
abstract: Eosinophilic leukocytes (eosinophils) are important effector cells in allergic inflammatory diseases such as asthma, in which significant accumulation of these cells is observed in the bronchial mucosa. However, there is little information about the relationships between bronchoconstriction and accumulation of eosinophils. We hypothesized that eosinophils are retained in the bronchial vasculature in the inner airway wall during bronchoconstriction because of deformation of the mucosal membrane. To test this hypothesis we induced unilateral bronchoconstriction in open chest guinea pigs by stimulating the right vagus nerve and compared the accumulation of eosinophils in the airway wall of the constricted and contralateral unconstricted lungs using histologic specimens. Results show that the density of eosinophils (number of cells/wall area) significantly increased in the inner wall and decreased in the adventitia of the constricted airways compared with the contralateral unconstricted airways. There was a positive relationship between the amount of smooth muscle shortening and the eosinophil density in the inner wall. On the other hand, this relationship was significantly negative in the adventitia. Atropine completely inhibited the eosinophil accumulation in the inner wall. These data suggest that eosinophils can accumulate in the airway inner wall during bronchoconstriction because of geometrical factors.
authors.affiliation: Respiratory Health Network of Center of Excellence, University of British Columbia, St. Paul's Hospital, Vancouver, Canada.
authors.forename: Y
authors.initials: Y
authors.lastname: Saito
authors.forename: M
authors.initials: M
authors.lastname: Okazawa
mesh_terms: D000818:Animals; D001285:Atropine / Q000494:pharmacology; D001980:Bronchi / Q000473:pathology*; D016084:Bronchoconstriction / Q000502:physiology*; D001993:Bronchodilator Agents / Q000494:pharmacology; D002452:Cell Count; D004558:Electric Stimulation; D004804:Eosinophils / Q000473:pathology*; D006168:Guinea Pigs; D008297:Male; D009130:Muscle, Smooth / Q000473:pathology; D014630:Vagus Nerve / Q000502:physiology*
chemical_list: D001993:Bronchodilator Agents; D001285:Atropine