Isolation and characterization of cytotoxic diterpenoid isomers from propolis.
The methanol extract of Brazilian propolis was fractionated by HPLC, based on HuH13 (human hepatocellular carcinoma) cell cytotoxicity assay. Two isomers of diterpenoid with a molecular formula of C20H30O3 (MW: 318.46) were isolated. The structures of these colorless compounds were determined as clerodane diterpenoids (I, 15-oxo-3, 13Z-kolavadien-17-oic acid; II, 15-oxo-3Z, 13E-kolavadien-17-oic acid).
Matsuno
T
T
National Institute of Health, Tokyo, Japan.
Matsumoto
Y
Y
Saito
M
M
Morikawa
J
J
D001938:Brazil; D006528:Carcinoma, Hepatocellular; D002470:Cell Survival / Q000187:drug effects*; D002851:Chromatography, High Pressure Liquid; D004224:Diterpenes / Q000737:chemistry* / Q000302:isolation & purification / Q000633:toxicity; D006367:HeLa Cells; D006801:Humans; D007624:KB Cells; D008113:Liver Neoplasms; D015394:Molecular Structure; D011429:Propolis / Q000737:chemistry* / Q000633:toxicity; D014407:Tumor Cells, Cultured
C110045:13-symphyoreticulic acid; D004224:Diterpenes; D011429:Propolis