Phototoxicity of some novel porphyrin hybrids against the human leukemic cell line TF-1.
Photodynamic induced cytotoxicity by porphyrin-DNA cross linker/intercalator hybrid diads and triads has been studied on the human leukemic cell line TF-1. Cells were incubated for 1 to 4 h with these new photosensitizers and irradiated with white light. Cell survival was assessed by the propidium iodide staining, using flow cytometry analysis. A comparison of the dark and light cell survival factor values suggests that irradiation has a significant effect on the toxicity at low concentrations for the porphyrin-chlorambucil diad and to a lesser extent at high concentrations for the porphyrin-acridone diad, the porphyrin-acridine diad and the porphyrin-cholic acid-chlorambucil triad. While the intrinsic antileukemic (via DNA cross-linking) activity of the chlorambucil moiety and the structural details may be responsible for the photoenhancement of the toxicity, the presence of acridine or acridone which are avid intercalators of DNA, is responsible for a similar effect seen for diads.
Viola
A
A
Laboratoire AM3-ESA-CNRS 6009, Faculté des Sciences Saint-Jérôme, Marseille, France.
Mannoni
P
P
Chanon
M
M
Julliard
M
M
Mehta
G
G
Maiya
B G
BG
Muthusamy
S
S
Sambaiah
T
T
D000166:Acridines / Q000494:pharmacology; D003432:Cross-Linking Reagents / Q000494:pharmacology; D003624:Darkness; D006801:Humans; D007364:Intercalating Agents / Q000494:pharmacology; D015394:Molecular Structure; D017319:Photosensitizing Agents / Q000494:pharmacology*; D011166:Porphyrins / Q000494:pharmacology*; D014407:Tumor Cells, Cultured
D000166:Acridines; D003432:Cross-Linking Reagents; D007364:Intercalating Agents; D017319:Photosensitizing Agents; D011166:Porphyrins