Xenopus oocytes express a unitary glutamate receptor endogenously.
The results described here demonstrate that Xenopus oocytes endogenously express a unitary glutamate receptor subunit XenU1. The level of XenU1 mRNA expression reaches approximately 1/300 of that in the adult Xenopus brain. The endogenous expression of XenU1, which can functionally interact with N-methyl-D-aspartate receptor subunit NR1, explains the differences in NR1 subunit expression in mammalian cell lines (no functional expression without partner subunits) and in the Xenopus oocytes (NR1 forms functional receptors when expressed singly).
Soloviev
M M
MM
Molecular Neurobiology Unit, Royal Free Hospital School of Medicine, London, UK.
Barnard
E A
EA
D000818:Animals; D001921:Brain / Q000378:metabolism; D017931:DNA Primers; D015870:Gene Expression; D016202:N-Methylaspartate / Q000378:metabolism; D009865:Oocytes / Q000378:metabolism*; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000378:metabolism; D017470:Receptors, Glutamate / Q000235:genetics* / Q000378:metabolism*; D016194:Receptors, N-Methyl-D-Aspartate / Q000378:metabolism; D029867:Xenopus Proteins; D014982:Xenopus laevis
D017931:DNA Primers; D012333:RNA, Messenger; D017470:Receptors, Glutamate; D016194:Receptors, N-Methyl-D-Aspartate; C101562:XenU1 protein, Xenopus; D029867:Xenopus Proteins; D016202:N-Methylaspartate