title: Duplication of a region in the multiple cloning site of a plasmid vector to enhance cloning-mediated addition of restriction sites to a DNA fragment.
authors.affiliation: Institut National de la Santé et de la Recherche Médicale, Faculté de Médecine Necker, Université Descartes, Paris, France.
authors.forename: L
authors.initials: L
authors.lastname: Cocea
mesh_terms: D001665:Binding Sites; D003001:Cloning, Molecular; D004247:DNA / Q000737:chemistry / Q000378:metabolism*; D004262:DNA Restriction Enzymes / Q000378:metabolism*; D015252:Deoxyribonucleases, Type II Site-Specific / Q000378:metabolism; D005822:Genetic Vectors; D010766:Phosphorylation; D010957:Plasmids
chemical_list: D004247:DNA; D004262:DNA Restriction Enzymes; C047020:endodeoxyribonuclease ScaI; C032824:ATCGAT-specific type II deoxyribonucleases; C025630:CAGCTG-specific type II deoxyribonucleases; C025651:CCCGGG-specific type II deoxyribonucleases; D015252:Deoxyribonucleases, Type II Site-Specific; C067488:GTMKAC-specific type II deoxyribonucleases