title: Lack of autocrine effects of IL-6 on human bone marrow stromal osteoprogenitor cells.
abstract: Interleukin-6 (IL-6) produced by osteoblastic cells plays an important role in the regulation of bone remodeling, mainly by stimulating osteoclast action. Although the IL-6 receptor is also found in osteoblastic cells, whether IL-6 exerts autocrine effects on osteoblastic cells is a matter of debate. This led us to study the effects of IL-6 on proliferation, osteoblastic activity as well as mRNA expression of various osteoblastic proteins in human bone marrow stromal osteoprogenitor cells (hBMSC). IL-6 did not affect cell proliferation assessed by [3H]-thymidine incorporation and osteoblastic activity determined by alkaline phosphatase activity and 45Ca incorporation. The expression of mRNAs for alkaline phosphatase, alpha 1 (I)-collagen, osteopontin and decorin also did not change significantly by IL-6 treatment. These results show that IL-6 does not have a significant autocrine role in regard to proliferation and osteoblastic activity of hBMSC.
authors.affiliation: Division of Endocrinology, Asan Medical Center, University of Ulsan College of Medicine, Seoul, Korea.
authors.forename: C H
authors.initials: CH
authors.lastname: Kim
authors.forename: S L
authors.initials: SL
authors.lastname: Cheng
authors.forename: G S
authors.initials: GS
authors.lastname: Kim
mesh_terms: D000469:Alkaline Phosphatase / Q000235:genetics; D015152:Blotting, Northern; D001854:Bone Marrow Cells / Q000166:cytology*; D002132:Calcium Radioisotopes; D002455:Cell Division; D002478:Cells, Cultured; D003094:Collagen / Q000235:genetics; D058575:Decorin; D016326:Extracellular Matrix Proteins; D006801:Humans; D015850:Interleukin-6 / Q000494:pharmacology*; D010006:Osteoblasts / Q000166:cytology*; D053495:Osteopontin; D011509:Proteoglycans / Q000235:genetics; D012333:RNA, Messenger / Q000378:metabolism; D012795:Sialoglycoproteins / Q000235:genetics; D013234:Stem Cells / Q000166:cytology*; D017154:Stromal Cells / Q000166:cytology*
chemical_list: D002132:Calcium Radioisotopes; C546878:DCN protein, human; D058575:Decorin; D016326:Extracellular Matrix Proteins; D015850:Interleukin-6; D011509:Proteoglycans; D012333:RNA, Messenger; C506895:SPP1 protein, human; D012795:Sialoglycoproteins; D053495:Osteopontin; D003094:Collagen; D000469:Alkaline Phosphatase