title: Implantation of a synthetic cornea: design, development and biological response.
abstract: Our goal was to evaluate 3 different designs of synthetic corneas in vivo. All devices had a transparent hydrogel center molded to a porous peripheral skirt. Over 30 devices were implanted into rabbits and followed for up to 6 months. The devices were preseeded with rabbit stromal fibroblasts, which enhanced the rate of fibroplasia. The anterior surface of the hydrogel was modified using argon rf plasma treatments. Clinical examinations were performed, and histological analyses were conducted on tissue throughout the time course. Our optimal model ranged from 4.5 to 6 mm and had an extended porous skirt increasing the surface area for fibroplasia and ultimate anchorage of the device. Fibroplasia occurred in this model, and collagen was detected by 28 days. The anterior chamber was normal with no detectable leakage of aqueous humor. Glycosaminoglycans were detected and followed the time course outlined previously when porous material itself was inserted into the stroma. We present the first demonstration that rabbit limbal epithelial cells can migrate onto the synthetic cornea in vivo.
authors.affiliation: Department of Ophthalmology, Boston University School of Medicine, Massachusetts 02118, U.S.A.
authors.forename: V
authors.initials: V
authors.lastname: Trinkaus-Randall
authors.forename: X Y
authors.initials: XY
authors.lastname: Wu
authors.forename: R
authors.initials: R
authors.lastname: Tablante
authors.forename: A
authors.initials: A
authors.lastname: Tsuk
mesh_terms: D000818:Animals; D002465:Cell Movement; D003094:Collagen / Q000378:metabolism; D003315:Cornea / Q000378:metabolism* / Q000473:pathology; D016039:Corneal Transplantation / Q000295:instrumentation* / Q000379:methods; D004847:Epithelial Cells / Q000502:physiology; D019573:Epithelium, Corneal / Q000378:metabolism; D005353:Fibronectins / Q000378:metabolism; D006497:Heparan Sulfate / Q000378:metabolism; D020136:Hydrogel, Polyethylene Glycol Dimethacrylate; D007150:Immunohistochemistry; D011092:Polyethylene Glycols; D011817:Rabbits; D016212:Transforming Growth Factor beta / Q000032:analysis
chemical_list: D003094:Collagen; D005353:Fibronectins; D006497:Heparan Sulfate; D020136:Hydrogel, Polyethylene Glycol Dimethacrylate; D011092:Polyethylene Glycols; D016212:Transforming Growth Factor beta