title: Molecular cloning of the porcine beta-1,2-N-acetylglucosaminyltransferase II gene and assignment to chromosome 1q23-q27.
abstract: Glycosyltransferases play an important role in the synthesis of glycoproteins. Here we report the isolation of a brain cDNA coding for 89% of the porcine UDP-N-acetylglucosamine:alpha-6-D-mannoside-beta-1,2-N-acetylglucosaminy ltransferase II (EC 2.4.1.143) (GnTII). The cDNA was used for screening a genomic liver DNA library and isolation of a recombinant lambda FIX II phage containing the complete porcine GnTII gene and upstream and downstream sequences. The beta-1,2-N-acetylglucosaminyltransferase II gene harbours a single exon with an open reading frame of 1338 bp coding for a 446 amino acid protein with a calculated molecular mass of 51.1 kDa. The promoter of the GnTII gene is lacking a TATA-box and shows variable transcription start sites. In the 3'-untranslated region a polymorphic polyadenosine stretch was detected. The porcine GnTII gene contains four polyadenylation sites. PCR analysis of a porcine-rodent hybrid cell panel revealed the chromosomal location of the GnTII gene on SSC 1q23-q27. The mapping data of the cell panel were confirmed by fluorescence in situ hybridization (FISH) on metaphase chromosomes.
authors.affiliation: Institute of Veterinary Medicine, University of Göttingen, Germany.
authors.forename: T
authors.initials: T
authors.lastname: Leeb
authors.forename: B
authors.initials: B
authors.lastname: Kriegesmann
authors.forename: B G
authors.initials: BG
authors.lastname: Baumgartner
authors.forename: C
authors.initials: C
authors.lastname: Klett
authors.forename: M
authors.initials: M
authors.lastname: Yerle
authors.forename: H
authors.initials: H
authors.lastname: Hameister
authors.forename: B
authors.initials: B
authors.lastname: Brenig
mesh_terms: D000818:Animals; D001483:Base Sequence; D001921:Brain / Q000201:enzymology*; D002874:Chromosome Mapping; D019976:Cloning, Organism; D017931:DNA Primers; D005091:Exons; D008969:Molecular Sequence Data; D017351:N-Acetylglucosaminyltransferases / Q000096:biosynthesis* / Q000737:chemistry / Q000235:genetics*; D016366:Open Reading Frames; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000737:chemistry; D012045:Regulatory Sequences, Nucleic Acid; D013552:Swine / Q000235:genetics*
chemical_list: D017931:DNA Primers; D011994:Recombinant Proteins; D017351:N-Acetylglucosaminyltransferases; C063313:alpha-1,6-mannosyl-glycoprotein beta-1,2-N-acetylglucosaminyltransferase