Expression and characterization of a trpl homolog from rat.
Mammalian homologues of the Drosophila trp/trpl-gene-family code for "Ca(2+)-store-operated" channels. Here we describe the cloning and expression of a trp/trpl homologous gene from rat brain. The clone is named Rtrp3 because of its high homology to the recently described Htrp3 (Zhu et al (1996) Cell 85:661-671). Expression of Rtrp3 in the mammalian COS-1 cell line leads to 100% increase of capacitative Ca2+ entry as compared to the controls. This capacitative calcium entry can be completely blocked with La3+ (10 microM). We conclude that Rtrp3, a new member of the growing family of trp/trpl homologues in mammalians, is involved in "Ca(2+)-store-operated" Ca2+ entry into the cell.
Preuss
K D
KD
2. Physiologisches Institut, Universität des Saarlandes, Homburg/Saar, Germany.
Nöller
J K
JK
Krause
E
E
Göbel
A
A
Schulz
I
I
D000595:Amino Acid Sequence; D000818:Animals; D001923:Brain Chemistry / Q000235:genetics; D019556:COS Cells; D015220:Calcium Channels / Q000235:genetics / Q000378:metabolism; D002148:Calmodulin-Binding Proteins / Q000096:biosynthesis* / Q000737:chemistry* / Q000235:genetics; D003001:Cloning, Molecular; D029721:Drosophila Proteins; D005822:Genetic Vectors; D006801:Humans; D008565:Membrane Proteins / Q000096:biosynthesis* / Q000737:chemistry* / Q000235:genetics; D008969:Molecular Sequence Data; D051381:Rats; D017386:Sequence Homology, Amino Acid; D050051:Transient Receptor Potential Channels
D015220:Calcium Channels; D002148:Calmodulin-Binding Proteins; D029721:Drosophila Proteins; D008565:Membrane Proteins; D050051:Transient Receptor Potential Channels; C074192:trpl protein, Drosophila