Failure of Neisseria gonorrhoeae to grow in the ATB NH susceptibility test system.
The ATB NH system designed for antibiotic susceptibility testing of Neisseria gonorrhoeae, Neisseria meningitidis and Haemophilus influenzae was evaluated using 94 clinical isolates of gonococci representing a wide variety of serovar/auxotype strains. Using the manufacturer's automated system 55% of the clinical isolates failed to grow, compared with a 33% failure rate for manual processing and visual reading. Growth failure was significantly higher with 1A isolates (73% automated and 69% manual) than with 1B isolates (49% automated and 25% manual). The higher failure rate of 1A isolates correlated with multiple auxotrophy. The inability of the ATB NH system to support the growth of common serovar/auxotypes makes the ATB NH system unsuitable for antibiotic susceptibility testing of N. gonorrhoeae.
Young
H
H
Scottish Neisseria gonorrhoeae Reference Laboratory, Department of Medical Microbiology, Edinburgh University Medical School, UK.
Moyes
A
A
Hardie
C
C
D006801:Humans; D008826:Microbial Sensitivity Tests; D009344:Neisseria gonorrhoeae / Q000187:drug effects / Q000254:growth & development*