High-performance liquid chromatographic determination of oxolinic acid in the plasma of seabass (Dicentrarchus labrax) anaesthetized with 2-phenoxyethanol.
A high-performance liquid chromatographic (HPLC) method for the determination of oxolinic acid (OA) in the plasma of seabass (Dicentrarchus labrax) anaesthetized with 2-phenoxyethanol is described. The samples were extracted and cleaned up by a solid-phase extraction procedure using C18 extraction cartridges. After the eluent was evaporated, the dry residue was dissolved in 1/15 M phosphate buffer. OA was determined by using an isocratic HPLC method with UV detection at 340 nm. Seabass drug-free plasma samples were spiked with OA at 0.2, 1.0, 5.0 and 25.0 microg/ml. Validation of the method showed good precision and accuracy. The mean recovery was 92.2%, with a relative standard deviation lower than 5%. The quantification limit was 0.2 microg/ml. The method was tested on 300 plasma samples of OA-treated seabass.
Loussouarn
S
S
Ecole Nationale Vétérinaire de Nantes, Laboratoire de Chimiothérapie Aquacole et Environnement, Nantes, France.
Pouliquen
H
H
Armand
F
F
D000777:Anesthetics; D000818:Animals; D001492:Bass; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D005026:Ethylene Glycols; D010093:Oxolinic Acid / Q000097:blood*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013056:Spectrophotometry, Ultraviolet
D000777:Anesthetics; D005026:Ethylene Glycols; C005398:phenoxyethanol; D010093:Oxolinic Acid