Application of the extracellular alpha-amylase gene from Streptococcus bovis 148 to construction of a secretion vector for yogurt starter strains.
Streptococcus thermophilus ATCC 19258, Lactobacillus delbrueckii subsp. bulgaricus T-11, and Lactococcus lactis subsp. lactis IL1403 were transformed with the alpha-amylase gene (amyA) from Streptococcus bovis 148 by using a wide host-range vector, and all the transformants secreted the alpha-amylase successfully. Since the promoter and the secretion signal of the amyA gene were functional in these strains, we constructed a secretion vector using the expression elements of amyA. Trials to secrete foreign enzymes in yogurt starter strains were performed using this novel secretion vector.
Satoh
E
E
Central Research Institute, Meiji Milk Products Co., Ltd., Odawara, Japan.
Ito
Y
Y
Sasaki
Y
Y
Sasaki
T
T
D005822:Genetic Vectors; D007778:Lactobacillus / Q000235:genetics*; D013294:Lactococcus lactis / Q000235:genetics*; D013291:Streptococcus / Q000235:genetics*; D016985:Streptococcus bovis / Q000235:genetics*; D014169:Transformation, Bacterial; D015014:Yogurt; D000516:alpha-Amylases / Q000235:genetics*
D000516:alpha-Amylases