Correlation between expression of platelet-derived endothelial cell growth factor (thymidine phosphorylase) and microvessel density in early-stage human colon carcinomas.
Platelet-derived endothelial cell growth factor may play a role in tumor development through its angiogenic action. To clarify the relationship between expression of platelet-derived endothelial cell growth factor and microvessel density in the development of human colon carcinoma, we examined 80 early-stage colon carcinomas using microscopy and immunohistochemistry. Localization of platelet-derived endothelial cell growth factor was assessed by immunocytochemistry, while microvessel count was evaluated by either HE staining or Factor VIII immunostaining. Among the examined carcinomas, 35 were classified as m carcinomas including carcinoma in situ, whereas 45 were sm carcinomas. Fifteen (42.9%) of the 35 m and 30 (66.7%) of the 45 sm carcinomas demonstrated high vascular density, whereas 20 (57.1%) m and 15 (33.3%) sm carcinomas showed moderate or low vascular density. Vascular density was higher in sm carcinomas than in m carcinomas and there was a significant correlation between depth of invasion and vascular density. Of the 45 highly vascularized carcinomas, 44 expressed platelet-derived endothelial cell growth factor. There was a statistically significant correlation between the frequency of platelet-derived endothelial cell growth factor expression and microvessel density (P = 0.012). These data demonstrate that microvessel density may be associated with the depth of cancer invasion and that platelet-derived endothelial cell growth factor may play an important role in the early stage of colon cancer development through angiogenesis.
Saeki
T
T
Department of Clinical Research, National Shikoku Cancer Center Hospital, Matsuyama, Japan.
Tanada
M
M
Takashima
S
S
Saeki
H
H
Takiyama
W
W
Nishimoto
N
N
Moriwaki
S
S
D002277:Carcinoma / Q000098:blood supply / Q000201:enzymology* / Q000473:pathology; D002278:Carcinoma in Situ / Q000098:blood supply / Q000201:enzymology / Q000473:pathology; D003110:Colonic Neoplasms / Q000098:blood supply / Q000201:enzymology* / Q000473:pathology; D004396:Coloring Agents; D004801:Eosine Yellowish-(YS); D005169:Factor VIII / Q000032:analysis; D005456:Fluorescent Dyes; D005753:Gastric Mucosa / Q000098:blood supply / Q000201:enzymology / Q000473:pathology; D015972:Gene Expression Regulation, Neoplastic; D006416:Hematoxylin; D006801:Humans; D007124:Immunoenzyme Techniques; D007150:Immunohistochemistry; D008833:Microcirculation / Q000473:pathology; D009361:Neoplasm Invasiveness; D009367:Neoplasm Staging; D009389:Neovascularization, Pathologic / Q000201:enzymology / Q000473:pathology; D013939:Thymidine Phosphorylase / Q000032:analysis* / Q000235:genetics
D004396:Coloring Agents; D005456:Fluorescent Dyes; D005169:Factor VIII; D013939:Thymidine Phosphorylase; D004801:Eosine Yellowish-(YS); D006416:Hematoxylin