Deletion of a consensus oestrogen response element half-site in the glucocorticoid receptor of human multiple myeloma.
We have carried out molecular scanning of the glucocorticoid receptor (GR) of the glucocorticoid resistant multiple myeloma cell line U266. An amplified fragment from the 3' untranslated region displayed an aberrant migration by PCR-single-stranded conformational polymorphism (PCR-SSCP) analysis. The mutant allele had a deletion of an 8 base pair sequence containing a half-site of an oestrogen response element. This motif was found conserved in rat GR. This same allele lacked four As in an upstream region with 18 consecutive As in the normal allele. These mutations may affect mRNA stability or alter interactions with regulatory factors.
Karkera
J D
JD
Medical Oncology Section, Department of Veterans Affairs Medical Center, Washington, D.C. 20422, U.S.A.
Taymans
S E
SE
Turner
G
G
Yoshikawa
T
T
Detera-Wadleigh
S D
SD
Wadleigh
R G
RG
D004967:Estrogens / Q000235:genetics*; D005091:Exons; D006801:Humans; D009101:Multiple Myeloma / Q000235:genetics*; D009154:Mutation; D016133:Polymerase Chain Reaction; D011965:Receptors, Glucocorticoid / Q000235:genetics*; D017384:Sequence Deletion; D014407:Tumor Cells, Cultured
D004967:Estrogens; D011965:Receptors, Glucocorticoid