Capillary zone electrophoresis with electrospray condensation particle counting detection.
A new sheath flow microelectrospray interface combines capillary electrophoretic separation of protein standards with condensation particle counting detection. Protein separations are performed in both coated and uncoated fused-silica columns. The electrospray needle utilizes a sheath flow rate between 0 and a few microL/min. Typically, the makeup flow is equal to or less than the electroosmotic flow from the separation capillary; thus there is minimal dilution while still providing pH adjustment and solid electrical contact with the separation capillary. While there are some inherent restrictions to a condensation particle counting detector, the microelectrospray needle performs well and has direct application to electrospray mass spectrometry.
Lewis
K C
KC
Department of Chemistry, University of North Carolina at Chapel Hill 27599-3290, USA.
Jorgenson
J W
JW
Kaufman
S L
SL
D000336:Aerosols; D000818:Animals; D002256:Carbonic Anhydrases / Q000302:isolation & purification; D002417:Cattle; D002645:Chickens; D019075:Electrophoresis, Capillary / Q000295:instrumentation* / Q000379:methods*; D004798:Enzymes / Q000302:isolation & purification; D004867:Equipment Design; D006736:Horses; D008832:Microchemistry / Q000295:instrumentation / Q000379:methods; D009113:Muramidase / Q000302:isolation & purification; D009211:Myoglobin / Q000302:isolation & purification; D009339:Needles; D011506:Proteins / Q000302:isolation & purification*
D000336:Aerosols; D004798:Enzymes; D009211:Myoglobin; D011506:Proteins; D009113:Muramidase; D002256:Carbonic Anhydrases