TNF-alpha induces apoptosis in rat fetal brown adipocytes in primary culture.
The effect of TNF-alpha on cell death in rat fetal brown adipocytes maintained in primary culture was determined. TNF-alpha inhibited proliferation and induced apoptosis in these cells. Most of the cells undergoing apoptosis after TNF-alpha treatment did not express PCNA, suggesting an induction of apoptosis by TNF-alpha in non-proliferative cells. IGF-I but not EGF prevented TNF-alpha-induced apoptosis.
Porras
A
A
Departamento de Bioquímica y Biología Molecular II, Instituto de Bioquímica, Facultad de Farmacia, Universidad Complutense, Madrid, Spain.
Alvarez
A M
AM
Valladares
A
A
Benito
M
M
D017667:Adipocytes / Q000166:cytology / Q000187:drug effects* / Q000502:physiology; D002001:Adipose Tissue, Brown / Q000187:drug effects* / Q000502:physiology; D000818:Animals; D017209:Apoptosis / Q000187:drug effects / Q000502:physiology*; D002455:Cell Division / Q000187:drug effects; D002478:Cells, Cultured; D004815:Epidermal Growth Factor / Q000494:pharmacology; D005333:Fetus; D007334:Insulin-Like Growth Factor I / Q000494:pharmacology; D007700:Kinetics; D018809:Proliferating Cell Nuclear Antigen / Q000032:analysis; D051381:Rats; D014409:Tumor Necrosis Factor-alpha / Q000037:antagonists & inhibitors / Q000494:pharmacology*
D018809:Proliferating Cell Nuclear Antigen; D014409:Tumor Necrosis Factor-alpha; D004815:Epidermal Growth Factor; D007334:Insulin-Like Growth Factor I