Effects of retinoids on the generation of neutrophil-derived reactive oxygen species studied by EPR spin trapping techniques.
OBJECTIVE
To study the potential efficacy of retinoids on granulocytes with regard to their role in inflammatory dermatoses.


METHODS
We investigated the in vitro effect of acitretin and isotretinoin on the generation of reactive oxygen species (ROS) by stimulated human neutrophils using EPR spin trapping techniques.


RESULTS
The effects of the two retinoids on ROS generation by neutrophils were different. Acitretin increased the generation of hydroxyl radicals, whereas isotretinoin showed an antioxidant activity against the superoxide anion.


CONCLUSIONS
Our study demonstrates retinoid type-dependent effects on ROS production by stimulated neutrophils.
Bohne
M
M
Department of Dermatology and Venereology, Otto-von-Guericke University, Magdeburg, Germany. Marisela.bohne@medizin.uni-magdeburg.de
Struy
H
H
Gerber
A
A
Gollnick
H
H
D017255:Acitretin / Q000494:pharmacology; D004578:Electron Spin Resonance Spectroscopy; D006801:Humans; D017665:Hydroxyl Radical / Q000378:metabolism; D015474:Isotretinoin / Q000494:pharmacology; D009504:Neutrophils / Q000187:drug effects* / Q000378:metabolism*; D017382:Reactive Oxygen Species / Q000378:metabolism*; D012176:Retinoids / Q000494:pharmacology*; D013481:Superoxides / Q000378:metabolism
D017382:Reactive Oxygen Species; D012176:Retinoids; D013481:Superoxides; D017665:Hydroxyl Radical; D015474:Isotretinoin; D017255:Acitretin