title: Heat-induced superaggregation of amphotericin B reduces its in vitro toxicity: a new way to improve its therapeutic index.
abstract: Superaggregation of amphotericin B (AmB) was previously shown to occur upon heating of solutions at 70 degrees C. In the present study, we demonstrate that heat pretreatment of Fungizone (deoxycholate salt of AmB [AmB-DOC]) solutions induces a drastic decrease in the in vitro toxicity of this antibiotic. Heated AmB-DOC colloidal solutions, which mainly contained superaggregated and monomeric forms of the antibiotic, were strongly less hemolytic than unheated solutions (aggregates and monomers). Thermal pretreatment of AmB-DOC solutions also reduced the toxicity to the cell line HT29, as deduced from two simultaneous cell viability assays (3-4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide and lactate dehydrogenase release). These heated colloidal solutions were only slightly less efficient than the unheated ones at inhibiting the growth of Candida albicans cells in vitro. Such results suggest that mild heat treatment of AmB-DOC solutions could provide a new and simple solution for improving the therapeutic index of this antifungal agent by reducing its toxicity to mammalian cells.
authors.affiliation: Laboratoire de Physicochimie Biomoléculaire et Cellulaire (CNRS UA 2056), Université P. et M. Curie, Paris, France. gaboriau@wanadoo.fr
authors.forename: F
authors.initials: F
authors.lastname: Gaboriau
authors.forename: M
authors.initials: M
authors.lastname: Chéron
authors.forename: C
authors.initials: C
authors.lastname: Petit
authors.forename: J
authors.initials: J
authors.lastname: Bolard
mesh_terms: D000666:Amphotericin B / Q000737:chemistry / Q000494:pharmacology / Q000633:toxicity*; D002176:Candida albicans / Q000187:drug effects / Q000254:growth & development; D002462:Cell Membrane / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D006358:Hot Temperature; D006801:Humans; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D014407:Tumor Cells, Cultured / Q000187:drug effects
chemical_list: D000666:Amphotericin B; D007770:L-Lactate Dehydrogenase