Age-dependent changes in astroglial reactivity in human ischemic stroke. Immunohistochemical study.
Clinical investigations revealed that a course of human ischemic stroke may be different in various patient's age. For the purpose of finding a reflection of this phenomenon in morphological picture we examined astroglial reactions in human brains with ischemic necrosis. Material was divided into two groups: senile (SG) (80-101 years, n = 34) and the middle age (MG) (40-65 years, n = 33). Astrocyte reactivity was evaluated using immunohistochemical methods with antibodies against: protein S-100, GFAP, Vimentin and recombinant parts of the Ki-67 molecule (MIB-1 antibody) during 32 days after the disease onset. Investigations revealed that astroglial reactivity appeared later (5th day) in SG then in MD (3rd day), was less pronounced-especially in the third (p < 0.05) and fourth week (p < 0.01), was more diffused and of shorter duration in SG than in MG. Astrocyte proliferation turned out surprisingly minimal in both groups and was observed more often in MG then in SG. We suggest that astroglial reactivity depends on the patient's age. Difference in the astrocyte reactions may be caused by a decrease of mitogenic and transforming factors activity, a decrease of astrocyte susceptibility for stimulating factors and/or ischemic tolerance phenomenon.
Dziewulska
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Department of Neurology, School of Medicine, Warszawa.
D000328:Adult; D000367:Age Factors; D000368:Aged; D000906:Antibodies / Q000032:analysis; D001253:Astrocytes / Q000276:immunology / Q000473:pathology*; D002545:Brain Ischemia / Q000473:pathology*; D046508:Culture Techniques; D006801:Humans; D007150:Immunohistochemistry; D008875:Middle Aged; D009336:Necrosis
D000906:Antibodies