title: Selection of a strain of Clostridium argentinense producing high titers of type G botulinal toxin.
abstract: The strain G89HT of Clostridium argentinense obtained by culture selection of the prototype G89 strain producing high titers of type G botulinal toxin was studied. Its cultural, biochemical and toxigenic characteristics and the presence of plasmids were tested. Both strains showed similar physiological features and carried a 83 MDa plasmid. A 170 MDa plasmid was also recognized in the G89HT strain. Notably, this strain was better sporulating and showed a higher toxigenicity than the prototype G89 C. argentinense strain. These two characteristics might permit a long term storage and perhaps yield high antitoxin titres.
authors.affiliation: Area de Microbiología, Universidad Nacional de San Luis, Argentina.
authors.forename: O
authors.initials: O
authors.lastname: Puig de Centorbi
authors.forename: H M
authors.initials: HM
authors.lastname: Quiroz
authors.forename: C
authors.initials: C
authors.lastname: Bogni
authors.forename: A
authors.initials: A
authors.lastname: Calzolari
authors.forename: H J
authors.initials: HJ
authors.lastname: Centorbi
mesh_terms: D001426:Bacterial Proteins / Q000032:analysis; D001905:Botulinum Toxins / Q000378:metabolism*; D003013:Clostridium / Q000254:growth & development / Q000378:metabolism* / Q000472:pathogenicity; D003470:Culture Media / Q000378:metabolism; D009500:Neutralization Tests; D010957:Plasmids / Q000235:genetics / Q000302:isolation & purification; D013171:Spores, Bacterial / Q000235:genetics / Q000254:growth & development / Q000378:metabolism; D014357:Trypsin / Q000378:metabolism; D014774:Virulence / Q000235:genetics
chemical_list: D001426:Bacterial Proteins; D003470:Culture Media; D014357:Trypsin; D001905:Botulinum Toxins