Inhibition of HIV-1 infection by the beta-chemokine MDC.
CD8(+) T lymphocytes from individuals infected with human immunodeficiency virus-type 1 (HIV-1) secrete a soluble activity that suppresses infection by HIV-1. A protein associated with this activity was purified from the culture supernatant of an immortalized CD8(+) T cell clone and identified as the beta-chemokine macrophage-derived chemokine (MDC). MDC suppressed infection of CD8(+) cell-depleted peripheral blood mononuclear cells by primary non-syncytium-inducing and syncytium-inducing isolates of HIV-1 and the T cell line-adapted isolate HIV-1IIIB. MDC was expressed in activated, but not resting, peripheral blood mononuclear cells and binds a receptor on activated primary T cells. These observations indicate that beta-chemokines are responsible for a major proportion of HIV-1-specific suppressor activity produced by primary T cells.
Pal
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Advanced BioScience Laboratories, 5510 Nicholson Lane, Kensington, MD 20895, USA.
Garzino-Demo
A
A
Markham
P D
PD
Burns
J
J
Brown
M
M
Gallo
R C
RC
DeVico
A L
AL
D000595:Amino Acid Sequence; D000998:Antiviral Agents / Q000276:immunology*; D015152:Blotting, Northern; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology*; D002118:Calcium / Q000097:blood; D002460:Cell Line; D002461:Cell Line, Transformed; D002478:Cells, Cultured; D054422:Chemokine CCL22; D019742:Chemokines, CC / Q000737:chemistry / Q000276:immunology* / Q000302:isolation & purification / Q000378:metabolism; D016655:HIV Core Protein p24 / Q000096:biosynthesis; D015658:HIV Infections / Q000276:immunology; D015497:HIV-1 / Q000276:immunology* / Q000502:physiology; D006801:Humans; D007963:Leukocytes, Mononuclear / Q000276:immunology / Q000378:metabolism / Q000821:virology*; D008213:Lymphocyte Activation; D019707:Receptors, Chemokine / Q000378:metabolism; D015728:Receptors, HIV / Q000378:metabolism; D013601:T-Lymphocytes / Q000276:immunology
D000998:Antiviral Agents; C515482:CCL22 protein, human; D054422:Chemokine CCL22; D019742:Chemokines, CC; D016655:HIV Core Protein p24; D019707:Receptors, Chemokine; D015728:Receptors, HIV; D002118:Calcium