title: Heterogeneous distribution of the unusual phospholipid semilysobisphosphatidic acid through the Golgi complex.
abstract: To investigate the distribution of lipids through the Golgi complex, we analyzed the envelopes of several viruses that assemble in different subcompartments of the Golgi, as well as subcellular fractions. Our results indicate that each Golgi subcompartment has a distinct phospholipid composition due mainly to differences in the relative amounts of semilysobisphosphatidic acid (SLBPA), sphingomyelin, phosphatidylserine, and phosphatidylinositol. Interestingly, SLBPA is enriched in the adjacent Golgi networks compared with the Golgi stack, and this enrichment varies with cell type. The heterogeneous distribution of SLBPA through the Golgi complex suggests it may play an important role in the structure and/or function of this organelle.
authors.affiliation: Department of Cell Biology and Anatomy, Johns Hopkins University School of Medicine, Baltimore, Maryland 21205-2105, USA.
authors.forename: E B
authors.initials: EB
authors.lastname: Cluett
authors.forename: E
authors.initials: E
authors.lastname: Kuismanen
authors.forename: C E
authors.initials: CE
authors.lastname: Machamer
mesh_terms: D000818:Animals; D002457:Cell Extracts; D002458:Cell Fractionation; D002460:Cell Line; D006224:Cricetinae; D004285:Dogs; D006056:Golgi Apparatus / Q000737:chemistry* / Q000821:virology; D007668:Kidney; D010712:Phosphatidic Acids / Q000032:analysis*; D010743:Phospholipids / Q000032:analysis; D012328:RNA Viruses / Q000378:metabolism; D017527:Receptor, IGF Type 2 / Q000032:analysis; D019065:Virus Assembly
chemical_list: D002457:Cell Extracts; D010712:Phosphatidic Acids; D010743:Phospholipids; D017527:Receptor, IGF Type 2; C021745:semilysobisphosphatidic acid