Comparison of conventional culture methods and two commercial enzyme immunoassays for detection of Salmonella in porcine fecal samples and cecal contents.
Two commercial enzyme immunoassays, designated EIA-1 and EIA-2, for the detection of salmonella in feces and cecal contents were compared to conventional culture methods. Out of 362 cecal content samples, 35 were positive by EIA-1 and 30 were positive by EIA-2 and conventional methods. Out of 189 fecal samples, 41 were positive by EIA-1, 30 were positive by EIA-2, and 24 were positive by conventional culture methods. The EIA-1 assay method detected more positive samples overall, although only comparison of the fecal samples was statistically significant. The results indicate that the EIA-1 method is rapid, requiring only 24 hours, and may be more sensitive than the EIA-2 assay and conventional culture techniques. Enzyme immunoassays for the detection of salmonella in fecal samples or cecal contents provide a reasonable alternative to conventional culture techniques.
Wegener
H C
HC
Danish Veterinary Laboratory, Copenhagen V, Denmark.
Baggesen
D L
DL
D000818:Animals; D002432:Cecum / Q000382:microbiology; D005243:Feces / Q000382:microbiology*; D005766:Gastrointestinal Contents / Q000382:microbiology*; D007124:Immunoenzyme Techniques; D011933:Reagent Kits, Diagnostic; D012475:Salmonella / Q000254:growth & development / Q000302:isolation & purification*; D012481:Salmonella Infections, Animal / Q000175:diagnosis*; D013552:Swine; D013553:Swine Diseases
D011933:Reagent Kits, Diagnostic