In vivo labeling of Schistosoma japonicum cercariae with 35S.
A technique was developed for in vivo labeling of adult Schistosoma japonicum worms using 35S bound to methionine and cysteine. The isotope was applied in an in vivo labeling system in which Oncomelania hupensis snails harboring a patent S. japonicum were individually exposed to the label. Following exposure to cercariae, mice were perfused after 6 wk. For autoradiography and fluorography, the worms were mounted on cardboard, covered with x-ray films, and exposed for 5 wk. With both techniques, images of both male and female worms were obtained in the 1- and 2-microCi groups, but not in the 0.5-microCi group. Fluorography proved more sensitive than autoradiography. Snail survival, labeled cercariae, and worm establishment were not affected by the radiolabeling. 35S was thus found useful for radiolabeling adult S. japonicum worms.
Johansen
M V
MV
Danish Bilharziasis Laboratory, Charlottenlund, Denmark.
Christensen
N O
NO
Nansen
P
P
D000818:Animals; D001345:Autoradiography; D003545:Cysteine / Q000737:chemistry; D005260:Female; D007553:Isotope Labeling / Q000379:methods*; D008297:Male; D008715:Methionine / Q000737:chemistry; D010779:Photofluorography; D012549:Schistosoma japonicum / Q000502:physiology*; D013462:Sulfur Radioisotopes
D013462:Sulfur Radioisotopes; D008715:Methionine; D003545:Cysteine