The Pseudomonas aeruginosa outer membrane protein I vaccine: immunogenicity and safe administration in man.
After expression in Escherichia coli and purification by Ni++ chelate-affinity chromatography, the outer membrane protein I (OprI) of Pseudomonas aeruginosa was tested in experimental animals for its safety and pyrogenicity. Four groups of 7 adult human volunteers were then vaccinated 3 times at four-weekly intervals with either 500 micrograms, 200 micrograms, 50 micrograms or 20 micrograms of OprI adsorbed onto aluminum hydroxide. The vaccinations were well tolerated and without systemic side effects, but a significant rise of antibody titers against OprI was measured in the serum of those who had received the 500 micrograms, 200 micrograms or 50 micrograms doses. Raised antibody titers against OprI were still present 30 weeks after the final vaccination. It was possible to demonstrate binding of the complement component C1q to the elicited antibodies, and this confirms their ability to promote antibody-mediated complement-dependent opsonization.
von Specht
B U
BU
Chirurgische Universitätsklinik, Chirurgische Forschung, Freiburg im Breisgau, Germany.
Lücking
H C
HC
Blum
B
B
Schmitt
A
A
Hungerer
K D
KD
Domdey
H
H
D000328:Adult; D000818:Animals; D000907:Antibodies, Bacterial / Q000096:biosynthesis / Q000097:blood*; D000917:Antibody Formation; D001426:Bacterial Proteins / Q000096:biosynthesis / Q000276:immunology*; D001428:Bacterial Vaccines / Q000009:adverse effects / Q000633:toxicity; D015922:Complement C1q / Q000378:metabolism; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D008074:Lipoproteins / Q000096:biosynthesis / Q000276:immunology*; D008297:Male; D011550:Pseudomonas aeruginosa / Q000276:immunology*; D051381:Rats; D017208:Rats, Wistar; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000276:immunology; D013997:Time Factors; D014614:Vaccines, Synthetic / Q000009:adverse effects / Q000633:toxicity
D000907:Antibodies, Bacterial; D001426:Bacterial Proteins; D001428:Bacterial Vaccines; D008074:Lipoproteins; D011994:Recombinant Proteins; D014614:Vaccines, Synthetic; C060507:outer membrane lipoprotein I, Bacteria; D015922:Complement C1q