title: High-efficiency T-vector cloning of PCR products by forced A tagging and post-ligation restriction enzyme digestion.
authors.affiliation: School of Life Science, QUT, Qld., Australia.
authors.forename: H
authors.initials: H
authors.lastname: Bielefeldt-Ohmann
authors.forename: D R
authors.initials: DR
authors.lastname: Fitzpatrick
mesh_terms: D003001:Cloning, Molecular / Q000379:methods*; D011088:DNA Ligases / Q000378:metabolism; D017931:DNA Primers; D003838:Deoxyadenine Nucleotides / Q000378:metabolism*; D015252:Deoxyribonucleases, Type II Site-Specific / Q000378:metabolism; D004926:Escherichia coli / Q000235:genetics; D005822:Genetic Vectors; D016133:Polymerase Chain Reaction; D019914:Taq Polymerase / Q000378:metabolism; D013942:Thymine Nucleotides / Q000378:metabolism*
chemical_list: D017931:DNA Primers; D003838:Deoxyadenine Nucleotides; D013942:Thymine Nucleotides; D019914:Taq Polymerase; D015252:Deoxyribonucleases, Type II Site-Specific; C027440:GATATC-specific type II deoxyribonucleases; D011088:DNA Ligases; C026600:2'-deoxyadenosine triphosphate; C024157:thymidine 5'-triphosphate