[Experimental study on human glioma SHG44 treated by HSV-tk gene therapy].
The paper reports the construction of retroviral vector pLNTK carrying HSV-tk gene driven by PGK promoter and the successful transfer into human glioma cell SHG44. The in vitro study confirmed that ayclovir (ACV) sensitive level of the gene-transferred glioma cell (SHGLNTK) was 1,000 times that of SHG44. 3H-TdR incorporation confirmed that the DNA replication in SHGLNTK was considerably suppressed when treated with ACV. The in vivo study confirmed that ACV could suppress tumor formation of the SHGLNTK cells. In situ gene transfer treatment of nude mice carrying SHG44 tumor showed good therapeutic results. Such treatment may be used as an innovative method for brain tumor therapy.
Pan
Y
Y
Department of Neurosurgery, First Affiliated Hospital of Suzhou Medical College.
Hui
G
G
Lu
D
D
D000212:Acyclovir / Q000627:therapeutic use; D000818:Animals; D000963:Antimetabolites / Q000627:therapeutic use; D001932:Brain Neoplasms / Q000473:pathology / Q000628:therapy*; D004273:DNA, Neoplasm / Q000096:biosynthesis; D018014:Gene Transfer Techniques; D015316:Genetic Therapy; D005910:Glioma / Q000473:pathology / Q000628:therapy*; D006801:Humans; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D009368:Neoplasm Transplantation; D013937:Thymidine Kinase / Q000235:genetics*; D014407:Tumor Cells, Cultured
D000963:Antimetabolites; D004273:DNA, Neoplasm; D013937:Thymidine Kinase; D000212:Acyclovir