A novel RING finger protein, BFP, predominantly expressed in the brain.
RING finger is a variant zinc finger motif present in a new family of proteins including transcription regulators. A genomic DNA fragment containing RING finger motifs was identified by the polymerase chain reaction using degenerate primers. Using this fragment as a probe, we have isolated a novel cDNA from rat brain library. The predicted open reading frame contains a RING finger domain at its N-terminal portion. The corresponding transcript was detected predominantly in the brain and therefore was designated brain finger protein (bfp). An antibody raised against a recombinant bfp reveals the presence of the bfp in the brain. Interestingly, the bfp is induced during retinoic acid-mediated differentiation of P19 embryonal carcinoma cells into neural cells. These findings suggest the possible involvement of bfp in some aspects of neural cell regulation.
Inoue
S
S
Department of Biochemistry, Saitama Medical School, Japan.
Orimo
A
A
Saito
T
T
Ikeda
K
K
Sakata
K
K
Hosoi
T
T
Orimo
H
H
Ouchi
Y
Y
Muramatsu
M
M
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D001921:Brain / Q000378:metabolism*; D004268:DNA-Binding Proteins / Q000096:biosynthesis* / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification; D054278:Embryonal Carcinoma Stem Cells; D018507:Gene Expression Regulation, Developmental / Q000187:drug effects; D006801:Humans; D051379:Mice; D008969:Molecular Sequence Data; D014411:Neoplastic Stem Cells / Q000187:drug effects / Q000378:metabolism; D009419:Nerve Tissue Proteins / Q000096:biosynthesis* / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification; D014176:Protein Biosynthesis; D011506:Proteins / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification; D051381:Rats; D014212:Tretinoin / Q000494:pharmacology; D014407:Tumor Cells, Cultured; D016335:Zinc Fingers
D004268:DNA-Binding Proteins; D009419:Nerve Tissue Proteins; D011506:Proteins; C109213:ring finger protein, BFP; D014212:Tretinoin