Surface plasmon resonance biomolecular interaction analysis mass spectrometry. 2. Fiber optic-based analysis.
Fiber optic probe-based surface plasmon resonance (SPR) detection has been used in combination with matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) mass spectrometry for the rapid, sensitive, and selective detection of biomolecules. SPR was used to monitor the covalent immobilization of a polyclonal antibody to the surface of a fiber optic probe. The derivatized probe was then used for the selective detection (from solution) of the corresponding antigen and a secondary antibody directed toward the antigen. Species retained during the SPR detection process were next analyzed by direct MALDI-TOF analysis of the probe surface (after exposed to the MALDI matrix and introduction into the mass spectrometer). The combined approach allowed for the two-dimensional detection of biomolecules, with SPR analysis yielding quantitative information pertinent to the binding events and MALDI-TOF providing details on the qualitative nature of the binding partners.
Nelson
R W
RW
Department of Chemistry and Biochemistry, Arizona State University, Tempe 85287-1604, USA.
Krone
J R
JR
Jansson
O
O
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D000937:Antigen-Antibody Reactions; D015374:Biosensing Techniques; D005336:Fiber Optic Technology; D006801:Humans; D007074:Immunoglobulin G / Q000276:immunology; D009211:Myoglobin / Q000276:immunology; D055100:Optical Fibers; D011817:Rabbits; D012680:Sensitivity and Specificity; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods*
D000911:Antibodies, Monoclonal; D007074:Immunoglobulin G; D009211:Myoglobin