title: Induction of the S100 chemotactic protein, CP-10, in murine microvascular endothelial cells by proinflammatory stimuli.
abstract: Microvascular endothelial cells (EC) have multiple functions in inflammatory responses, including the production of chemoattractants that enhance leukocyte transmigration into tissues. Chemotactic protein, 10 kD (CP-10), is an S100 protein with potent chemotactic activity for myeloid cells in vitro and in vivo and is expressed in neutrophils and lipopolysaccharide (LPS)-activated macrophages. We show here that CP-10 is induced in murine endothelioma cell lines (bEnd-3, sEnd-1, and tEnd-1) after activation with LPS and interleukin-1 (IL-1) but not tumor necrosis factor alpha (TNFalpha) or interferon gamma (IFNgamma). Induction was not mediated by endogenous release of IL-1 or TNFalpha and was not directly upregulated by phorbol myristate acetate, calcium ionophore, or vitamin D3. EC were exquisitely sensitive to IL-1 activation (3.4 U/mL) and CP-10 mRNA induction with IL-1 occurred earlier (8 hours) than with LPS (12 hours). Furthermore, some microvessels and capillaries in delayed-type hypersensitivity lesions expressed cytoplasmic CP-10. Responses to LPS and not IL-1 in vitro were regulated by the degree of cell confluence and by TNFalpha costimulation. The related MRP-14 mRNA had a different induction pattern. Monomeric and homodimeric CP-10 upregulated by activation was predominantly cell-associated. EC-derived CP-10 may contribute to amplification of inflammatory processes by enhancing leukocyte shape changes and transmigration in the microcirculation.
authors.affiliation: School of Pathology, The University of New South Wales, New South Wales, Australia.
authors.forename: T
authors.initials: T
authors.lastname: Yen
authors.forename: C A
authors.initials: CA
authors.lastname: Harrison
authors.forename: J M
authors.initials: JM
authors.lastname: Devery
authors.forename: S
authors.initials: S
authors.lastname: Leong
authors.forename: S E
authors.initials: SE
authors.lastname: Iismaa
authors.forename: T
authors.initials: T
authors.lastname: Yoshimura
authors.forename: C L
authors.initials: CL
authors.lastname: Geczy
mesh_terms: D000818:Animals; D002135:Calcium-Binding Proteins / Q000096:biosynthesis* / Q000235:genetics; D040501:Calgranulin A; D002630:Chemotactic Factors / Q000096:biosynthesis*; D004730:Endothelium, Vascular / Q000378:metabolism*; D005786:Gene Expression Regulation; D008070:Lipopolysaccharides / Q000494:pharmacology; D051379:Mice; D008833:Microcirculation / Q000378:metabolism; D012333:RNA, Messenger / Q000032:analysis; D009418:S100 Proteins; D014407:Tumor Cells, Cultured
chemical_list: D002135:Calcium-Binding Proteins; D040501:Calgranulin A; D002630:Chemotactic Factors; D008070:Lipopolysaccharides; D012333:RNA, Messenger; D009418:S100 Proteins; C086145:S100a8 protein, mouse