Coexpression of interleukin-8 receptors in head and neck squamous cell carcinoma.
BACKGROUND
Interleukin 8 (IL-8) is an important cytokine involved in tumor growth and angiogenesis in a variety of malignancies. We hypothesize that IL-8 plays an important role in the cellular proliferation and angiogenesis seen in head and neck squamous cell carcinoma (HNSCC) and set out to identify its receptors, IL-8RA and IL-8RB.


METHODS
Immunohistochemical analysis was performed on specimens from 38 HNSCC patients with stage I to IV disease and control tissues.


RESULTS
All of cancer specimens demonstrated positive staining for IL-8RA. The IL-8RA staining of microvessel endothelial cells was seen in 51%. The IL-8RB pattern was similar to the IL-8RA pattern in that 97% of cancer sections demonstrated positive cancer cell staining, and 74% of the specimens demonstrated positive staining for microvessel endothelial cells.


CONCLUSIONS
Our studies demonstrate that IL-8 receptors are expressed by cancer cells and microvessel endothelial cells in HNSCC, suggesting that IL-8 may act in an autocrine/paracrine fashion to stimulate cellular proliferation and angiogenesis.
Richards
B L
BL
Department of Surgery, University of Connecticut Health Center, School of Medicine, Farmington 06030-3105, USA.
Eisma
R J
RJ
Spiro
J D
JD
Lindquist
R L
RL
Kreutzer
D L
DL
D000818:Animals; D015703:Antigens, CD / Q000096:biosynthesis*; D002294:Carcinoma, Squamous Cell / Q000378:metabolism* / Q000473:pathology; D004730:Endothelium, Vascular / Q000378:metabolism / Q000473:pathology; D006258:Head and Neck Neoplasms / Q000378:metabolism* / Q000473:pathology; D006801:Humans; D007150:Immunohistochemistry; D016209:Interleukin-8 / Q000096:biosynthesis*; D051379:Mice; D008819:Mice, Nude; D009368:Neoplasm Transplantation; D019707:Receptors, Chemokine / Q000096:biosynthesis; D018123:Receptors, Interleukin / Q000096:biosynthesis*; D023062:Receptors, Interleukin-8A; D023063:Receptors, Interleukin-8B; D014183:Transplantation, Heterologous
D015703:Antigens, CD; D016209:Interleukin-8; D019707:Receptors, Chemokine; D018123:Receptors, Interleukin; D023062:Receptors, Interleukin-8A; D023063:Receptors, Interleukin-8B