title: Sequence of the non-phosphorylating glyceraldehyde-3-phosphate dehydrogenase from Nicotiana plumbaginifolia and phylogenetic origin of the gene family.
abstract: A cDNA-library has been constructed from Nicotiana plumbaginifolia seedlings, and the non-phosphorylating glyceraldehyde-3-phosphate dehydrogenase (GapN, EC 1.2.1.9) was isolated by plaque hybridization using the cDNA from pea as a heterologous probe. The cDNA comprises the entire GapN coding region. A putative polyadenylation signal is identified. Phylogenetic analysis based on the deduced amino acid sequences revealed that the GapN gene family represents a separate ancient branch within the aldehyde dehydrogenase superfamily. It can be shown that the GapN gene family and other distinct branches of the superfamily have its phylogenetic origin before the separation of primary life-forms. This further demonstrates that already very early in evolution, a broad diversification of the aldehyde dehydrogenases led to the formation of the superfamily.
authors.affiliation: Institut für Genetik, Technische Universität Braunschweig, Germany.
authors.forename: A
authors.initials: A
authors.lastname: Habenicht
authors.forename: A
authors.initials: A
authors.lastname: Quesada
authors.forename: R
authors.initials: R
authors.lastname: Cerff
mesh_terms: D000444:Aldehyde Dehydrogenase / Q000235:genetics; D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000235:genetics; D001483:Base Sequence; D015139:Blotting, Southern; D003001:Cloning, Molecular; D017343:Genes, Plant; D005987:Glyceraldehyde-3-Phosphate Dehydrogenases / Q000235:genetics*; D008969:Molecular Sequence Data; D005810:Multigene Family; D010766:Phosphorylation; D010802:Phylogeny; D010940:Plant Proteins / Q000235:genetics*; D010947:Plants, Toxic; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid; D013295:Streptococcus mutans / Q000235:genetics; D014026:Nicotiana / Q000201:enzymology*
chemical_list: D001426:Bacterial Proteins; D010940:Plant Proteins; D005987:Glyceraldehyde-3-Phosphate Dehydrogenases; D000444:Aldehyde Dehydrogenase