Monoclonal antibodies against the plant cytokinin, dihydrozeatin riboside.
Three monoclonal antibodies (all IgG1) were prepared against the plant cytokinin dihydrozeatin riboside (diH[9R]Z) and characterized for suitability in the isolation and quantification of dihydrozeatin-type phytohormones. These antibodies detected as little as 5-10 pg of the homologous cytokinin when used in both competitive and noncompetitive immunoassays and each had characteristic crossreactivity with structurally-related cytokinins. One antibody was used to quantify HPLC-purified diH[9R]Z recovered from wheat tissue. The same antibody also was linked to an insoluble support for use in affinity purification of a mixture of cytokinins. These antibodies will be useful for purification and quantification of dihydrozeatin and its 9-riboside and 9-glucoside in plant tissue.
Banowetz
G M
GM
USDA Agricultural Research Service, Corvallis, OR 97331, USA.
Hess
J R
JR
Carman
J G
JG
D000818:Animals; D000911:Antibodies, Monoclonal; D003429:Cross Reactions; D003583:Cytokinins / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006825:Hybridomas / Q000378:metabolism; D051379:Mice; D008807:Mice, Inbred BALB C; D010088:Oxidoreductases / Q000378:metabolism; D014908:Triticum / Q000737:chemistry; D015026:Zeatin / Q000378:metabolism
D000911:Antibodies, Monoclonal; D003583:Cytokinins; D015026:Zeatin; D010088:Oxidoreductases; C019984:cytokinin oxidase