Enzyme immunoassay for myelin basic protein in cerebrospinal fluid.
Myelin basic proteins (MBPs) are a set of proteins making up about 30% of the protein content of the central nervous system myelin. Four human isoforms have been identified. The most abundant is a highly conserved 18.5 kDa polypeptide. For this species, the amino acid sequence homologies between human and monkey or human and chick are 98.2% and 71.1%, respectively. As a consequence, there is a very good immunological cross-reactivity between the mammalian MBP. This protein has been extensively used to induce experimental allergic encephalomyelits (EAE) in numerous animals. The evolution of chronic EAE in animal is similar to that of multiple sclerosis (MS), a demyelinating human pathology, and chronic EAE is considered to be an animal model of MS. In demyelinating pathologies, MBP concentration in the cerebrospinal fluid (CSF) is considered to be a good marker of demyelination. MBP concentration, in biological fluids, is generally determined by radioimmunoassay (RIA). The RIA technique currently used is highly sensitive (0.1-2.5 ng/ml) but has the drawback of requiring the handling of radioactivity and frequent labelling of MBP. So we developed a new enzyme immunoassay (EIA) technique. Our technique has the same sensitivity as RIA, needs only small volumes of CSF (50 microliters) and the enzyme-labelled MBP tracer is stable for at least 12 months.
Najeme
F
F
Laboratoire d'Immunologie Moléculaire, Bâtiment 1b-Carreire Nord, Université de Bordeaux II, France.
Julien
J
J
Herblot
S
S
Dousset
V
V
Brochet
B
B
Bonnet
J
J
D000469:Alkaline Phosphatase / Q000134:cerebrospinal fluid / Q000378:metabolism; D000818:Animals; D005260:Female; D007124:Immunoenzyme Techniques; D008252:Macaca fascicularis; D008297:Male; D004676:Myelin Basic Protein / Q000134:cerebrospinal fluid* / Q000378:metabolism; D011817:Rabbits; D012015:Reference Standards
D004676:Myelin Basic Protein; D000469:Alkaline Phosphatase