Interaction between echovirus 7 and its receptor, decay-accelerating factor (CD55): evidence for a secondary cellular factor in A-particle formation.
Soluble forms of decay-accelerating factor (DAF) (CD55), the receptor for echovirus 7, were synthesized in the yeast Pichia pastoris. Purified recombinant protein containing SCR domains 2, 3, and 4, but lacking the serine/threonine rich region, was shown to block infection of susceptible cells by echovirus 7. In contrast to the situation with poliovirus and its receptor, the neutralization of echovirus 7 by soluble DAF was completely reversible and did not lead to the formation of 135S A-particles. Binding of virus to susceptible cells, by contrast, did lead to the formation of A particles, mainly from virus that had been internalized. The data suggest that a secondary factor(s) may contribute to A-particle formation and uncoating of echovirus 7.
Powell
R M
RM
School of Animal and Microbial Sciences, University of Reading, Whiteknights, United Kingdom.
Ward
T
T
Evans
D J
DJ
Almond
J W
JW
D018958:CD55 Antigens / Q000235:genetics / Q000378:metabolism*; D029822:Enterovirus B, Human / Q000378:metabolism*; D006367:HeLa Cells; D006801:Humans; D009500:Neutralization Tests; D017955:Poliovirus / Q000502:physiology; D011991:Receptors, Virus / Q000235:genetics / Q000378:metabolism*; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000378:metabolism; D017384:Sequence Deletion; D014771:Virion / Q000502:physiology
D018958:CD55 Antigens; D011991:Receptors, Virus; D011993:Recombinant Fusion Proteins