A highly stable methyl cellulose coating for capillary electrophoresis.
A highly stable capillary-coating has been created by coupling covalently a hydroxyl group of methyl cellulose to the epoxy group of alpha-glycidoxypropyltrimethoxysilane attached to a piece of fused silica tubing. This coating strongly suppresses electroendosmotic flow (EOF) and minimizes sample adsorption onto the capillary wall for several weeks or months. The stability of the coating has been tested with isoelectric focusing and free zone electrophoresis. The coupling procedure is easy to perform, and the coating withstands at least 135 consecutive runs under extreme pH conditions (2-12). EOF is still reduced after placing the coated capillary in 0.01 M NaOH for 30 days. A detailed description of the coating procedure is provided.
Liao
J L
JL
Department of Biochemistry, University of Uppsala, Sweden.
Abramson
J
J
Hjertén
S
S
D019075:Electrophoresis, Capillary / Q000295:instrumentation / Q000379:methods*; D008747:Methylcellulose
D008747:Methylcellulose