Dengue virus infection of human T lymphocytes.
We have previously reported (Kurane et al., Arch. Virol. 110, 91-101, 1990) that human T cell leukemia and lymphoma cell lines could be infected with dengue virus. In the present study we examined whether human T lymphocytes which are not leukemia or lymphoma cells can be infected with dengue virus. Dengue virus-specific human CD4+ T cell clones, JK44 and JK49, and CD8+ T cell clones, CB2.8 and CB6.17, were infected with dengue-2 virus. Dengue virus antigen-positive cells were detected in each of the four T cell lines by the immunofluorescence staining 20-60 hrs post infection (p.i). Infectious dengue virus was detected in the culture fluids 40 hrs p.i. These results demonstrate that activated CD4+ and CD8+ human T lymphocytes can be infected with dengue-2 virus.
Mentor
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Department of Medicine, University of Massachusetts Medical Center, Worcester 01545, USA.
Kurane
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D000330:Aedes / Q000821:virology; D000818:Animals; D000956:Antigens, Viral / Q000032:analysis / Q000096:biosynthesis; D015496:CD4-Positive T-Lymphocytes / Q000821:virology; D018414:CD8-Positive T-Lymphocytes / Q000821:virology; D002460:Cell Line; D002999:Clone Cells; D003716:Dengue Virus / Q000502:physiology*; D006801:Humans; D013601:T-Lymphocytes / Q000821:virology*; D014407:Tumor Cells, Cultured; D014779:Virus Replication
D000956:Antigens, Viral