Heme peroxidase activity measured in single mosquitoes identifies individuals expressing an elevated oxidase for insecticide resistance.
Optimum conditions are described for a simple, rapid, microplate-based assay that indirectly measures the differences in oxidase levels between individual susceptible, resistant, or induced mosquitoes. A small proportion (0.01-0.1) of a single mosquito is used, allowing multiple replicates of the oxidase assay. Cytochrome C is used as a positive control. The levels of oxidase found in sample populations of pyrethroid-susceptible, pyrethroid-resistant, and phenobarbital-induced Anopheles albimanus mosquitoes are characterized with the assay.
Brogdon
W G
WG
Entomology Branch F-22, Centers for Disease Control and Prevention, Chamblee, GA 30341, USA.
McAllister
J C
JC
Vulule
J
J
D000818:Animals; D000852:Anopheles / Q000201:enzymology*; D003578:Cytochrome-c Peroxidase / Q000032:analysis; D005260:Female; D007305:Insecticide Resistance; D010088:Oxidoreductases / Q000032:analysis; D009195:Peroxidase / Q000032:analysis*; D011153:Population
D010088:Oxidoreductases; D003578:Cytochrome-c Peroxidase; D009195:Peroxidase