title: Spirochaetes and other bacterial species associated with bovine digital dermatitis.
abstract: The 16S rRNA genes from spirochaetes associated with digital dermatitis of British cattle were amplified by polymerase chain reaction from digital dermatitis lesion biopsies using one universal and one treponeme-specific primer. Two treponemal sequences were identified both of which shared a high degree of homology with the oral pathogen Treponema denticola (98%). Two further 16S rRNA gene sequences were obtained and shared similarity to Bacteroides levii (99%) and Mycoplasma hyopharyngis (98%). Polymerase chain reaction with T. denticola-specific primers amplified a potential virulence gene from digital dermatitis lesions which shared a high degree of homology to the 46-kDa haemolysin gene of T. denticola. The significance of the presence of organisms in digital dermatitis lesions of the bovine foot which are closely related to oral pathogens is discussed.
authors.affiliation: Bacteriology Department, Central Veterinary Laboratory, Addlestone, Surrey, UK. RJCollighan@VLA.MAFF.GOV.UK
authors.forename: R J
authors.initials: RJ
authors.lastname: Collighan
authors.forename: M J
authors.initials: MJ
authors.lastname: Woodward
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001439:Bacteroides / Q000235:genetics; D001483:Base Sequence; D002417:Cattle; D002418:Cattle Diseases / Q000382:microbiology*; D017931:DNA Primers / Q000235:genetics; D005260:Female; D005534:Foot Diseases / Q000382:microbiology / Q000662:veterinary*; D005798:Genes, Bacterial; D006460:Hemolysin Proteins / Q000235:genetics; D008969:Molecular Sequence Data; D009055:Mouth / Q000382:microbiology; D009174:Mycoplasma / Q000235:genetics; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D012329:RNA, Bacterial / Q000235:genetics; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017386:Sequence Homology, Amino Acid; D013045:Species Specificity; D013143:Spirochaetaceae / Q000235:genetics / Q000302:isolation & purification* / Q000472:pathogenicity*; D013145:Spirochaetales Infections / Q000382:microbiology / Q000662:veterinary*; D014208:Treponema / Q000235:genetics / Q000302:isolation & purification / Q000472:pathogenicity
chemical_list: D017931:DNA Primers; D006460:Hemolysin Proteins; D012329:RNA, Bacterial; D012336:RNA, Ribosomal, 16S