Monoclonal antibody interaction with the third immunoglobulin-like domain of N-CAM is sufficient to cause cell migration.
Cellular adhesion molecules can influence a variety of biological mechanisms in the nervous system. These range from the processes of normal development and maintenance to neural plasticity and recovery following injury. The elucidation of the intricate contributions of these molecules will require the correlation of functional assays with specific molecules and the specific binding domains of such molecules with multiple signaling pathways. The data presented in this paper show that the monoclonal antibody anti-NCAM16, directed against the third immunoglobulin-like domain of the neural cell adhesion molecule N-CAM, is capable of stimulating the complex biological process of cell migration in primary embryonic motor neurons and human neuronal cell lines.
Ackley
R L
RL
Becton Dickinson Research Center, Research Triangle Park, North Carolina 27709, USA.
Madison
R D
RD
Archibald
S J
SJ
Hemperly
J J
JJ
D000595:Amino Acid Sequence; D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology* / Q000494:pharmacology; D002465:Cell Movement / Q000502:physiology; D002478:Cells, Cultured; D006801:Humans; D007136:Immunoglobulins / Q000737:chemistry*; D009046:Motor Neurons / Q000187:drug effects / Q000502:physiology; D009132:Muscles / Q000737:chemistry; D009414:Nerve Growth Factors / Q000494:pharmacology; D019006:Neural Cell Adhesion Molecules / Q000737:chemistry* / Q000276:immunology* / Q000494:pharmacology; D009474:Neurons / Q000187:drug effects / Q000502:physiology*; D010446:Peptide Fragments / Q000737:chemistry / Q000235:genetics / Q000276:immunology*; D051381:Rats / Q000196:embryology; D017207:Rats, Sprague-Dawley; D011993:Recombinant Fusion Proteins / Q000737:chemistry / Q000494:pharmacology; D014020:Tissue Extracts / Q000494:pharmacology; D014407:Tumor Cells, Cultured
D000911:Antibodies, Monoclonal; D007136:Immunoglobulins; D009414:Nerve Growth Factors; D019006:Neural Cell Adhesion Molecules; D010446:Peptide Fragments; D011993:Recombinant Fusion Proteins; D014020:Tissue Extracts