Sequence analysis of functional regions of homoserine dehydrogenase genes from L-lysine and L-threonine-producing mutants of Brevibacterium lactofermentum.
The homoserine dehydrogenase (HD) genes from Brevibacterium lactofermentum lysine- and threonine-producing mutants were cloned, using the polymerase chain reaction, and sequenced. We found the amino acid substitutions, Val104Ile in the lysine-producing mutants in which HD may cause leaky mutation and Ser393Phe in the threonine-producing mutant with feedback-insensitive HD.
Sugimoto
M
M
Central Research Laboratories, Ajinomoto Co., Inc., Kawasaki, Japan.
Tanaka
A
A
Suzuki
T
T
Matsui
H
H
Nakamori
S
S
Takagi
H
H
D000595:Amino Acid Sequence; D001483:Base Sequence; D001951:Brevibacterium / Q000235:genetics* / Q000378:metabolism; D004269:DNA, Bacterial / Q000235:genetics; D005798:Genes, Bacterial / Q000235:genetics*; D006715:Homoserine Dehydrogenase / Q000737:chemistry* / Q000235:genetics; D008239:Lysine / Q000235:genetics / Q000378:metabolism; D008969:Molecular Sequence Data; D009154:Mutation / Q000235:genetics; D013912:Threonine / Q000235:genetics / Q000378:metabolism
D004269:DNA, Bacterial; D013912:Threonine; D006715:Homoserine Dehydrogenase; D008239:Lysine