Induction of surface-associated proteins of Streptococcus uberis by cultivation with extracellular matrix components and bovine mammary epithelial cells.
Surface-associated protein expression by Streptococcus uberis was influenced by the presence of collagen, laminin and bovine mammary epithelial cells in the culture medium. After electrophoresis and silver staining, four proteins stained more intensively in samples from S. uberis cultivated with epithelial cells and extracellular matrix components than in samples from S. uberis cultivated alone. Induction of these proteins was more obvious after multiple bacterial passages. The correlation between the phenotype of S. uberis and its potential virulence status as illustrated by an immunoblotting study with sera obtained from infected cows revealed that these proteins are probably expressed in vivo during infection.
Gilbert
F B
FB
Department of Animal Science, University of Tennessee, Knoxville 37901-1071, USA.
Luther
D A
DA
Oliver
S P
SP
D000818:Animals; D000942:Antigens, Bacterial / Q000737:chemistry; D001426:Bacterial Proteins / Q000096:biosynthesis* / Q000737:chemistry / Q000276:immunology; D002417:Cattle; D002478:Cells, Cultured; D003094:Collagen / Q000378:metabolism; D004847:Epithelial Cells / Q000382:microbiology; D005109:Extracellular Matrix / Q000378:metabolism; D005260:Female; D007797:Laminin / Q000378:metabolism; D008321:Mammary Glands, Animal / Q000382:microbiology*; D008414:Mastitis, Bovine / Q000209:etiology* / Q000517:prevention & control; D008970:Molecular Weight; D010641:Phenotype; D013291:Streptococcus / Q000276:immunology / Q000378:metabolism* / Q000472:pathogenicity*; D014774:Virulence
D000942:Antigens, Bacterial; D001426:Bacterial Proteins; D007797:Laminin; D003094:Collagen