title: Characterization of highly radiosensitive cell lines from a human ovarian small-cell cancer.
abstract: Cells were obtained at paracentesis from a patient with a rapidly growing ovarian tumor. A monolayer cell line (V7S), a xenograft tumor line (V7), and subsequently a xenograft-derived monolayer cell line (V7M) were established. Histological and immunohistochemical studies of the original tumor, xenograft, and cell lines provided a diagnosis of small-cell carcinoma of the ovary-which is consistent with the clinical course of the patient. V7S and V7M had a predominantly hypodiploid karyotype with a small tetraploid population. The V7M, which has been in long-term culture, also showed a nonrandom translocation involving chromosomes 1 and 14 and monosomy of X. Radiobiologically, V7S, V7, and V7M showed marked radiosensitivity with surviving fractions at 2 Gy, measured by clonogenic assay, of between 0.022 and 0.147. Split-dose experiments provided evidence that this radiosensitivity was not due to an inability in cellular repair. In vivo data from the xenograft (V7) revealed a highly radiosensitive tumor, corroborating the in vitro studies.
authors.affiliation: Research Laboratories, Velindre Hospital NHS Trust, Whitchurch, Cardiff, CF4 7XL.
authors.forename: J A
authors.initials: JA
authors.lastname: Hanson
authors.forename: E M
authors.initials: EM
authors.lastname: Parry
authors.forename: A R
authors.initials: AR
authors.lastname: Gibbs
authors.forename: J B
authors.initials: JB
authors.lastname: Court
authors.forename: M A
authors.initials: MA
authors.lastname: Pour-Faizi
authors.forename: I J
authors.initials: IJ
authors.lastname: Kerby
authors.forename: E A
authors.initials: EA
authors.lastname: Bean
mesh_terms: D018288:Carcinoma, Small Cell / Q000235:genetics / Q000473:pathology* / Q000532:radiotherapy; D002871:Chromosome Banding; D005260:Female; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D007621:Karyotyping; D010051:Ovarian Neoplasms / Q000235:genetics / Q000473:pathology* / Q000532:radiotherapy; D011836:Radiation Tolerance; D014178:Translocation, Genetic; D014407:Tumor Cells, Cultured