Calcium-lowering action of glucocorticoids in adrenalectomized-parathyroidectomized rats. Specificity and relative potency of natural and synthetic glucocorticoids.
The specificity and potency of glucocorticoids to lower serum calcium (Ca) in rats after parathyroidectomy (PTX) and adrenalectomy (ADX) were examined. Rats fasted overnight were given sc injections of various steroids immediately after the operations. The fall in serum calcium 5 h after PTX-ADX in rats given hypocalcemic doses of corticosterone was compared to that after injection of a test steroid. At high doses, progesterone, estradiol, testosterone, and aldosterone were inactive, whereas glucocorticoids were consistently hypocalcemic. These results indicate that the Ca-lowering effect is specific for steroids with glucocorticoid activity. Potency estimates were made by comparing the dose-response of natural and synthetic glucocorticoids to that of corticosterone, the major glucocorticoid in rats. The mean potency of hydrocortisone was 8.2 times that of corticosterone. Prednisolone was about 9.6, triamcinolone 33, betamethasone 109, and dexamethasone 301 times as potent as corticosterone. Thus, the use of the calcium-lowering action as a bioassay has provides a specific and rapid in vivo method to compare potencies of glucocorticoids consistent with those obtained by anti-inflammatory and glycogen deposition assays. The importance of this interesting calcitonin-like action of glucocorticoids in normal physiology of calcium metabolism is not yet established.
Mahgoub
A
A
Department of Pharmacology, School of Medicine, University of North Carolina, Chapel Hill 27599-7455, USA.
Hirsch
P F
PF
Munson
P L
PL
D000315:Adrenalectomy; D000818:Animals; D001623:Betamethasone / Q000008:administration & dosage / Q000494:pharmacology; D001681:Biological Assay; D002118:Calcium / Q000097:blood*; D015331:Cohort Studies; D003345:Corticosterone / Q000008:administration & dosage / Q000494:pharmacology; D003907:Dexamethasone / Q000008:administration & dosage / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D005260:Female; D005938:Glucocorticoids / Q000008:administration & dosage / Q000494:pharmacology*; D006854:Hydrocortisone / Q000008:administration & dosage / Q000494:pharmacology; D007279:Injections, Subcutaneous; D008297:Male; D016105:Parathyroidectomy; D011239:Prednisolone / Q000008:administration & dosage / Q000494:pharmacology; D011245:Pregnadienes / Q000008:administration & dosage / Q000494:pharmacology; D051381:Rats; D017207:Rats, Sprague-Dawley; D014221:Triamcinolone / Q000008:administration & dosage / Q000494:pharmacology
D005938:Glucocorticoids; D011245:Pregnadienes; D014221:Triamcinolone; D003907:Dexamethasone; D001623:Betamethasone; D011239:Prednisolone; D002118:Calcium; D003345:Corticosterone; D006854:Hydrocortisone