title: Immobilized cells with endocellular enzymes improved by the permealizing and crosslinking treatment.
abstract: In order to achieve high activity of enzymatic catalysis, the method of permealizing and crosslinking treatment has been suggested for immobilizing cells with endocellular enzymes. Polyethene-polyamine and glutardehyde were used to enhance the activity and stability of E. coli having aspartase entrapped in k-carrageenan gel and intact P. dacunhae cells having aspartate-beta-decarbonoxylase. The strength and enlargement effects, which favor the performance of catalysis in a packed bed reactor and a membrane reactor, respectively, were also obtained from the experiment results.
authors.affiliation: Department of Food Engineering, Dalian Institute of Light Industry, China.
authors.forename: Y
authors.initials: Y
authors.lastname: Shi
authors.forename: Y
authors.initials: Y
authors.lastname: Wu
authors.forename: F
authors.initials: F
authors.lastname: Jin
authors.forename: F
authors.initials: F
authors.lastname: Yan
authors.forename: X
authors.initials: X
authors.lastname: Yu
authors.forename: Q
authors.initials: Q
authors.lastname: Yuan
mesh_terms: D001220:Aspartate Ammonia-Lyase; D019149:Bioreactors; D002262:Carboxy-Lyases; D002351:Carrageenan; D002384:Catalysis; D002463:Cell Membrane Permeability; D018914:Cells, Immobilized; D003432:Cross-Linking Reagents; D004926:Escherichia coli / Q000187:drug effects*; D005782:Gels; D011549:Pseudomonas / Q000187:drug effects*
chemical_list: D003432:Cross-Linking Reagents; D005782:Gels; D002351:Carrageenan; D002262:Carboxy-Lyases; C019747:aspartate 4-decarboxylase; D001220:Aspartate Ammonia-Lyase