Expression of ICAM-1 in implanted primary and metastatic squamous cell carcinomas in rats.
FF6 tumor cells are derived from a spontaneous rat squamous cell carcinoma (SCC) which originally arose in the facial skin of a DA rat. In this study, FF6 tumor cells were implanted into rat oral mucosa to establish an ex vivo metastatic model. We analyzed the expression of intercellular cell adhesion molecule-1 (ICAM-1) in the implanted primary and metastatic FF6 tumors by immuno-staining with a monoclonal antibody (mAb) against ICAM-1. The implanted primary FF6 cells showed strong expression of ICAM-1, whereas the tumor cells of metastatic lesions showed weak or negative expression of ICAM-1. By immunostaining with mAb OX6, a number of MHC class II-positive macrophages were detected in tumor mesenchyme and surrounding the metastatic foci. These results suggested that the local immune reaction in the lymph node influenced the expression of ICAM-1 on tumor cells, and that MHC class II-positive macrophages may play a role in transplanted tumor growth and metastases.
Li
X
X
Department of Oral and Maxillofacial Surgery, Yamaguchi University School of Medicine, Ube, Japan.
Fujikura
Y
Y
Wang
Y H
YH
Sawada
T
T
Tokuda
N
N
Lovely
R S
RS
Hayatsu
Y
Y
Fukumoto
T
T
Shinozaki
F
F
D000818:Animals; D000911:Antibodies, Monoclonal; D015704:CD4 Antigens / Q000032:analysis; D015496:CD4-Positive T-Lymphocytes / Q000473:pathology; D016827:CD8 Antigens / Q000032:analysis; D018414:CD8-Positive T-Lymphocytes / Q000473:pathology; D002294:Carcinoma, Squamous Cell / Q000473:pathology* / Q000556:secondary; D004396:Coloring Agents; D004801:Eosine Yellowish-(YS); D005153:Facial Neoplasms / Q000473:pathology; D005456:Fluorescent Dyes; D015972:Gene Expression Regulation, Neoplastic; D006416:Hematoxylin; D000949:Histocompatibility Antigens Class II / Q000032:analysis; D007124:Immunoenzyme Techniques; D018799:Intercellular Adhesion Molecule-1 / Q000032:analysis* / Q000235:genetics; D008048:Lip Neoplasms / Q000473:pathology; D008198:Lymph Nodes / Q000276:immunology / Q000473:pathology; D008207:Lymphatic Metastasis / Q000473:pathology; D008264:Macrophages / Q000276:immunology / Q000473:pathology; D008648:Mesoderm / Q000473:pathology; D009062:Mouth Neoplasms / Q000473:pathology / Q000556:secondary; D009368:Neoplasm Transplantation; D011897:Random Allocation; D051381:Rats; D011919:Rats, Inbred Strains; D014062:Tongue Neoplasms / Q000473:pathology; D014407:Tumor Cells, Cultured
D000911:Antibodies, Monoclonal; D015704:CD4 Antigens; D016827:CD8 Antigens; D004396:Coloring Agents; D005456:Fluorescent Dyes; D000949:Histocompatibility Antigens Class II; D018799:Intercellular Adhesion Molecule-1; D004801:Eosine Yellowish-(YS); D006416:Hematoxylin