Structure of a phosphorylated polysaccharide from Shewanella putrefaciens strain S29.
A phosphorylated polysaccharide was isolated from the aqueous layer of the phenol-water extract of a non-halophilic bacterium Shewanella putrefaciens strain S29. The glycosyl phosphate linkage in the polysaccharide was split under mild acid conditions to give, after borohydride reduction, a phosphorylated oligosaccharide-alditol. On the basis of sugar analysis and 1H, 13C and 31P NMR spectroscopy, including 2D COSY, relayed COSY, rotating-frame NOE spectroscopy (ROESY), heteronuclear 13C,1H COSY, and H-detected heteronuclear 1H,31P multiple-quantum coherence (HMQC), it was concluded that the polysaccharide is built up of tetrasaccharide-phosphate repeating units having the following structure: [sequence: see text] where QuiNAc and Qui4NAc are 2-acetamido-2,6-dideoxyglucose and 4-acetamido-4,6-dideoxyglucose, respectively.
Shashkov
A S
AS
N.D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, Moscow, Russian Federation.
Senchenkova SyN
Nazarenko
E L
EL
Zubkov
V A
VA
Gorshkova NyM
Knirel
Y A
YA
Gorshkova
R P
RP
D002240:Carbohydrate Sequence; D002247:Carbon Isotopes; D008401:Gas Chromatography-Mass Spectrometry; D006092:Gram-Negative Facultatively Anaerobic Rods / Q000737:chemistry*; D006600:Hexosephosphates; D008969:Molecular Sequence Data; D019906:Nuclear Magnetic Resonance, Biomolecular; D009844:Oligosaccharides / Q000737:chemistry; D010766:Phosphorylation; D011135:Polysaccharides, Bacterial / Q000737:chemistry*
D002247:Carbon Isotopes; D006600:Hexosephosphates; D009844:Oligosaccharides; D011135:Polysaccharides, Bacterial