Photodynamic therapy of the ciliary body with tin ethyl etiopurpurin and tin octaethyl benzochlorin in pigmented rabbits.
OBJECTIVE
The authors used a pigmented rabbit model to investigate two photosensitizers, tin ethyl etiopurpurin (SnET2) and tin octaethyl benzochlorin (BNZ 203), to determine their potential for creating ciliary body injuries during photodynamic therapy (PDT).


METHODS
The biodistribution of SnET2 (n = 10) and BNZ 203 (n = 9) was studied by fluorescence microscopy using a low light detection system, based on charged-coupled device photography, with digital image processing at 1 and 24 hours after injection. PDT with SnET2 (n = 8; 664 +/- 7-nm light; 75 mW/cm2; 50 or 100 J/cm2; 1-mm spot size) and BNZ 203 (n = 6; 689 nm; 75 mW/cm2; 50 or 100 J/cm2; 1-mm spot size) was performed at 24 hours post-injection. The control subjects for SnET2 (n = 5) and BNZ 203 (n = 3) were given a maximal light dose (100 J/cm2).


RESULTS
Both photosensitizers demonstrated an intravascular distribution at 1 hour that shifted to a ciliary body distribution at 24 hours (SnET2 much greater than BNZ 203). In addition, the SnET2 demonstrated suborgan localization to the nonpigmented ciliary body epithelium. Both photosensitizing agents were able to produce selective injury to the rabbit ciliary body (SnET2 much greater than BNZ 203), with evidence of a small component of thermal damage (SnET2 greater than BNZ 203).


CONCLUSIONS
PDT with SnET2 or BNZ 203 can produce selective injury to the pigmented rabbit ciliary body. The nonpigmented ciliary body epithelium exhibits selective retention of SnET2. This finding warrants further investigation.
Hill
R A
RA
Department of Ophthalmology, University of California, Irvine 92697-4375, USA.
Crean
D H
DH
Doiron
D R
DR
Ghosheh
F
F
Ryan
J A
JA
Kelly
H
H
Booth
M
M
Liaw
L H
LH
Newman
L
L
Berns
M W
MW
D000818:Animals; D002924:Ciliary Body / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D003905:Deuteroporphyrins / Q000493:pharmacokinetics / Q000494:pharmacology*; D004848:Epithelium / Q000187:drug effects / Q000378:metabolism / Q000473:pathology; D053685:Laser Therapy; D008856:Microscopy, Fluorescence; D009947:Organotin Compounds / Q000493:pharmacokinetics / Q000494:pharmacology*; D010778:Photochemotherapy; D017319:Photosensitizing Agents / Q000493:pharmacokinetics / Q000494:pharmacology*; D011166:Porphyrins / Q000493:pharmacokinetics / Q000494:pharmacology; D011817:Rabbits
D003905:Deuteroporphyrins; D009947:Organotin Compounds; D017319:Photosensitizing Agents; D011166:Porphyrins; C109855:tin octaethyl benzochlorin; C063166:tin etiopurpurin