Subcutaneous implantation of human post-transplant lymphoproliferative disease lesions in SCID mice.
The tumorigenic potential of six post-transplant B-cell lymphoproliferative disease (PTLD) lesions was evaluated in SCID mice. Three animals developed local subcutaneous B cell tumours, two of which contained Epstein-Barr virus (EBV) DNA. Two animals developed CD3 positive thymic neoplasms, and one mouse developed an uncharacterized spontaneously regressing subcutaneous tumour. Immunoglobulin gene rearrangements studies with a JH probe, and EBV clonality studies with a Bam NJ fused terminal probe, showed only one mouse tumour to be genealogically related to the corresponding clinical lesion. It is concluded that lymphoid clones which constitute human PTLD are not autonomous, but sustained by host-derived growth stimuli distinct from those operating in the SCID mouse mileu.
Randhawa
P S
PS
Department of Pathology, University of Pittsburgh Medical Center, PA 15213, USA.
Pietrzak
B
B
Nalesnik
M A
MA
Demetris
A J
AJ
Locker
J
J
D000328:Adult; D000818:Animals; D001402:B-Lymphocytes / Q000473:pathology; D015139:Blotting, Southern; D004279:DNA, Viral / Q000032:analysis; D005260:Female; D015326:Gene Rearrangement, B-Lymphocyte, Heavy Chain; D006566:Herpesviridae Infections / Q000150:complications; D004854:Herpesvirus 4, Human / Q000235:genetics; D006801:Humans; D007279:Injections, Subcutaneous; D008232:Lymphoproliferative Disorders / Q000209:etiology / Q000473:pathology* / Q000821:virology; D008297:Male; D051379:Mice; D016513:Mice, SCID; D008875:Middle Aged; D009368:Neoplasm Transplantation; D014180:Transplantation / Q000009:adverse effects*; D014183:Transplantation, Heterologous; D014412:Tumor Virus Infections / Q000150:complications
D004279:DNA, Viral