title: Cardiac myocyte membrane wounding in the abruptly pressure-overloaded rat heart under high wall stress.
abstract: The potential role of transient sarcolemmal membrane wounding as a signal transduction event for cardiomyocyte hypertrophy was evaluated in rats with short-term pressure overload caused by banding of the proximal aorta. This procedure resulted in significant increases in left ventricular systolic (1.5-fold) and end-diastolic (2.6-fold) pressures and wall stresses that were associated with significant wall thinning and cavitary enlargement. Quantitative image analysis of frozen sections of the stressed ventricles obtained 60 minutes after banding demonstrated a 6- to 10-fold increase in cytosolic staining with a horseradish peroxidase-labeled anti-albumin antibody compared with sham-operated controls, indicating that an increase in transient sarcolemmal membrane permeability (wounding) is an early response to an abrupt increase in hemodynamic load in vivo. We conclude that an intense hemodynamic stress in vivo can result in histologically detectable cardiomyocyte wounding.
authors.affiliation: Department of Medicine, Brigham and Women's Hospital, Harvard Medical School, Boston, Mass 02115, USA.
authors.forename: T A
authors.initials: TA
authors.lastname: Fischer
authors.forename: P L
authors.initials: PL
authors.lastname: McNeil
authors.forename: R
authors.initials: R
authors.lastname: Khakee
authors.forename: P
authors.initials: P
authors.lastname: Finn
authors.forename: R A
authors.initials: RA
authors.lastname: Kelly
authors.forename: M A
authors.initials: MA
authors.lastname: Pfeffer
authors.forename: J M
authors.initials: JM
authors.lastname: Pfeffer
mesh_terms: D000818:Animals; D001794:Blood Pressure; D001810:Blood Volume; D002462:Cell Membrane / Q000648:ultrastructure; D005260:Female; D006973:Hypertension / Q000473:pathology* / Q000503:physiopathology; D009206:Myocardium / Q000473:pathology*; D051381:Rats; D017208:Rats, Wistar; D013314:Stress, Mechanical