title: The role of tissue-fixed macrophages in apoptosis in the developing kidney.
abstract: Programmed cell death (PCD) and cell type involving dying cells in the developing mouse and human fetal kidneys were investigated using the modified TdT-mediated dUTP-biotin nick end-labeling (TUNEL) method in combination with immunohistochemistry. We identified many TUNEL-positive apoptotic cells mainly in the nephrogenic region in the developing mouse and human fetal kidneys. Tissue-fixed macrophages were widely distributed throughout the developing mouse and human fetal kidneys, including the nephrogenic zone. Most of the apoptotic cells in the nephrogenic zone were phagocytosed by tissue-fixed macrophages, suggesting that tissue-fixed macrophages play an important role in the process of PCD in the developing mouse kidney and human fetal kidney.
authors.affiliation: Department of Pediatrics, Okayama University Medical School, Japan.
authors.forename: M
authors.initials: M
authors.lastname: Yasui
authors.forename: H
authors.initials: H
authors.lastname: Tanaka
authors.forename: Y
authors.initials: Y
authors.lastname: Seino
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal; D015703:Antigens, CD / Q000032:analysis; D015214:Antigens, Differentiation, Myelomonocytic / Q000032:analysis; D017209:Apoptosis / Q000502:physiology*; D018845:CD11 Antigens / Q000032:analysis; D053938:DNA Fragmentation; D006801:Humans; D007150:Immunohistochemistry; D007668:Kidney / Q000166:cytology / Q000196:embryology / Q000254:growth & development*; D008264:Macrophages / Q000276:immunology / Q000502:physiology*; D051379:Mice; D008807:Mice, Inbred BALB C; D008813:Mice, Inbred ICR; D008856:Microscopy, Fluorescence; D010587:Phagocytosis / Q000502:physiology; D011419:Propidium; D000097382:CD68 Molecule
chemical_list: D000911:Antibodies, Monoclonal; D015703:Antigens, CD; D015214:Antigens, Differentiation, Myelomonocytic; D018845:CD11 Antigens; D011419:Propidium; C067980:CD68 antigen, human; D000097382:CD68 Molecule