title: Fates of the blastomeres of the 32-cell stage Pleurodeles waltl embryo.
abstract: Cell fate of the blastomeres at the 32-cell stage in the Pleurodeles waltl embryo was analyzed by injection of rhodamine or fluorescein lysinated-dextran (RLDx or FLDx). At the tailbud stage, the progeny of each blastomere contributed to more than one germ layer with unequal distribution along the anteroposterior and dorsoventral axis. Such a regionalized positioning of the descendants of the 32-cell blastomeres was found in the neuroectoderm, the epidermis, the notochord, the somites, the lateral plate, and the endoderm, but not in the head mesenchyme, the pronephros, or the blood islands. Results of double labeling of juxtaposed blastomeres showed that cell mixing and rearrangement take place during organ formation. Results are compared with those of the 32-cell stage fate map in Xenopus and Rana and reveal the more restricted fate of 32-cell stage blastomeres in Pleurodeles germ layers.
authors.affiliation: Biologie Moléculaire et Cellulaire du Développement, Groupe de Biologie Expérimentale, URA 1135 CNRS, Université Pierre et Marie Curie, Paris, France. mdelarue@ccr.jussieu.fr
authors.forename: M
authors.initials: M
authors.lastname: Delarue
authors.forename: F J
authors.initials: FJ
authors.lastname: Sáez
authors.forename: K E
authors.initials: KE
authors.lastname: Johnson
authors.forename: J C
authors.initials: JC
authors.lastname: Boucaut
mesh_terms: D000818:Animals; D001757:Blastomeres / Q000166:cytology*; D002460:Cell Line; D003911:Dextrans; D016650:Fluorescein-5-isothiocyanate / Q000031:analogs & derivatives; D005456:Fluorescent Dyes; D010999:Pleurodeles / Q000196:embryology*; D011898:Ranidae; D012235:Rhodamines; D014981:Xenopus
chemical_list: D003911:Dextrans; D005456:Fluorescent Dyes; D012235:Rhodamines; C044226:fluorescein isothiocyanate lysine-dextran; D016650:Fluorescein-5-isothiocyanate