Identification and characterization of radiation damaged products of monophosphate nucleosides by on-line capillary isotachophoresis-electrospray ionization mass spectrometry.
Analysis of trace levels of DNA damage as a biomarker of human exposure to radiation levels represents a significant challenge. Capillary isotachophoresis (CITP) is well suited for this application due to its high separation efficiency, large sample loading capacity, and relative ease of interfacing with highly specific and sensitive mass spectrometric detectors. This study describes the use of reversed anionic CITP coupled on-line with electrospray ionization-mass spectrometry (ESI-MS) for the identification and characterization of small quantities of radiation induced damage to deoxynucleotide monophosphates. CITP with ESI-tandem MS also provides structural information on the nature of the damage in addition to the molecular weight. This approach has the attraction of allowing the direct on-line determination of damaged nucleotides, as well as the free bases and nucleosides, and avoids the need for sample hydrolysis and/or derivatization.
Zhao
Z
Z
Merck Research Laboratories, West Point, PA, USA.
Fuciarelli
A F
AF
Smith
R D
RD
D015415:Biomarkers; D004247:DNA / Q000032:analysis / Q000528:radiation effects*; D004249:DNA Damage; D019075:Electrophoresis, Capillary / Q000295:instrumentation / Q000379:methods*; D004781:Environmental Exposure; D006801:Humans; D011834:Radiation Monitoring / Q000379:methods*; D012265:Ribonucleotides / Q000032:analysis / Q000528:radiation effects*; D018629:Spectrometry, Mass, Secondary Ion / Q000295:instrumentation / Q000379:methods*
D015415:Biomarkers; D012265:Ribonucleotides; D004247:DNA