Study on the production of a xylanolytic complex from Penicillium canescens 10-10c.
We screened about a hundred microorganisms (including unidentified yeasts, fungi, and bacteria) for their ability to produce xylanolytic enzymes. About 40 of them were hemicellulolytic; among these, we selected Penicillium canescens 10-10c for detailed study because of its ability to produce an interesting enzymatic complex in quantity. The xylanase complex was cellulase-free, and had an optimal activity at pH 4.6-5.0 and 55-60 degrees C on birchwood xylan. The best production was on soya meal and wheat straw; expression of the xylanase was repressed by glucose, xylose, and lactose. The optimization of culture medium and mode (fed-batch) enabled us to improve the production three to four times. The importance of the mixing conditions on the biomass and xylanase production is also reported.
Gaspar
A
A
Lesaffre Development, Marcq-En-Baroeul, France.
Cosson
T
T
Roques
C
C
Thonart
P
P
D005433:Flour; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D010407:Penicillium / Q000201:enzymology* / Q000254:growth & development; D013379:Substrate Specificity; D014908:Triticum; D014934:Wood; D043325:Xylan Endo-1,3-beta-Xylosidase; D014990:Xylans / Q000378:metabolism; D014995:Xylosidases / Q000096:biosynthesis / Q000302:isolation & purification / Q000378:metabolism*
D014990:Xylans; D014995:Xylosidases; D043325:Xylan Endo-1,3-beta-Xylosidase