title: In-vitro activity of topoisomerase inhibitors against Pneumocystis carinii.
abstract: The activity of eight topoisomerase inhibitors was investigated against five clinical isolates of Pneumocystis carinii. Susceptibility tests were performed by inoculation of the organisms on to a cell monolayer and parasites were counted after 72 h incubation at 37 degrees C. Culture plates were added with Dulbecco's modified Eagle's medium containing serial dilutions of lomefloxacin, norfloxacin, ofloxacin, pefloxacin, rufloxacin, camptothecin, amsacrine and etoposide. Atovaquone, pentamide isethionate and co-trimoxazole were used as control drugs. Etoposide gave inhibition comparable to that observed with atovaquone. Poor activity was demonstrated by pefloxacin, while the other topoisomerase inhibitors had no significant effect.
authors.affiliation: Institute of Infectious Diseases & Public Health, University of Ancona, Italy.
authors.forename: O
authors.initials: O
authors.lastname: Cirioni
authors.forename: A
authors.initials: A
authors.lastname: Giacometti
authors.forename: M
authors.initials: M
authors.lastname: Quarta
authors.forename: G
authors.initials: G
authors.lastname: Scalise
mesh_terms: D004791:Enzyme Inhibitors / Q000494:pharmacology*; D008826:Microbial Sensitivity Tests; D011010:Pneumocystis / Q000187:drug effects*; D059004:Topoisomerase I Inhibitors; D059005:Topoisomerase II Inhibitors
chemical_list: D004791:Enzyme Inhibitors; D059004:Topoisomerase I Inhibitors; D059005:Topoisomerase II Inhibitors