Resistance to herpes simplex virus type 1 and its latent infection of human T cell lymphotropic virus type I-transformed T cell lines of rabbits.
Fourteen T cell lines of rabbits were infected with herpes simplex virus type 1 (HSV-1) and examined for their susceptibility to lytic infection and ability to support virus replication. T cell lines of CD4+ 8- phenotype were more vulnerable to lysis and supported higher levels of virus replication than those of other phenotypes. Cell lines of CD4+ 8+ and CD4- 8+ phenotypes continued to proliferate, while remaining productively infected, for more than a month. These latently infected cell lines could be established by treatment with anti-HSV-1 serum and complement. Viral genes were only partially expressed and the CD8 membrane antigen was down-regulated. Infected cell lines, as well as peripheral blood lymphocytes, were shown to induce meningoencephalitis when inoculated intravenously into syngeneic hosts, suggesting a possible role for infected lymphocytes in HSV-1 transport in vivo.
Seto
A
A
Department of Microbiology, Faculty of Medicine, Shiga University of Medical Science, Otsu, Japan. seto@belle.shiga-med.ac.jp
Nagano
Y
Y
Isono
T
T
Kurokawa
M
M
D000818:Animals; D002461:Cell Line, Transformed; D002472:Cell Transformation, Viral; D006561:Herpes Simplex; D018259:Herpesvirus 1, Human; D015368:Human T-lymphotropic virus 1; D006801:Humans; D011817:Rabbits; D013601:T-Lymphocytes / Q000821:virology*; D017735:Virus Latency