title: Methods for studying the glucose-6-phosphate dehydrogenase activity in brain areas.
abstract: This paper reports on the protocols for the spectrophotometric determination of the enzymatic activity, the electrophoretic pattern and the cytochemical assay of glucose-6-phosphate dehydrogenase (G6PD) in different areas of rat brain. For the spectrophotometric assay we used the method of Glock and McLean. Non-denaturing polyacrylamide gel electrophoresis was performed using 1.5 mm thick slab-gel, with a 7.5% acrylamide separating gel and a 4% acrylamide stacking gel. NADP (0.01 mM) was included at the cathode buffer. The cytochemical assay was performed on unfixed cryostat sections, by incubating the slides for 5 min at 37 degrees C in the staining medium. A combination of these techniques offers a means for the study of the effect of age, drugs, oxidizing or reducing agents on the G6PD activity in different brain areas.
authors.affiliation: Istituto di Chimica Biologica G. Fornaini, Università di Urbino, Italy.
authors.forename: P
authors.initials: P
authors.lastname: Ninfali
authors.forename: G
authors.initials: G
authors.lastname: Aluigi
authors.forename: A
authors.initials: A
authors.lastname: Pompella
mesh_terms: D000818:Animals; D001921:Brain / Q000201:enzymology*; D004586:Electrophoresis; D005954:Glucosephosphate Dehydrogenase / Q000378:metabolism*; D006651:Histocytochemistry; D007527:Isoenzymes / Q000378:metabolism; D009462:Neurology / Q000379:methods*; D051381:Rats; D017207:Rats, Sprague-Dawley; D014018:Tissue Distribution
chemical_list: D007527:Isoenzymes; D005954:Glucosephosphate Dehydrogenase