[Induction of differentiation of human gastric cancer cell line SGE-7901 by retinamide].
Vitamin A and its analogus, the retinoids, are agents that are known to induce differentiation and inhibit growth on cell in vitro and in vivo. These agents show promise as prophylactic and therapeutic ones in human cancer and were noted extensively by scholars abroad, but the research has just been developed in recent years in our country. Retinamide (named briefly R II), a kind of new retinoids made in China, can induce differentiation and inhibit growth of cell, but its toxic effect is smaller than its maternal chemical compound (retinoic acid). Using automatic image analysis technology, polyacrylamide gel LDH isoenzyme electrophoresis, radioimmunoassay and condensing reaction of Con A, we have observed induction of differentiation of the R II on SGC-7901 cells. Our experiments indicate that R II can inhibit cell proliferation, decrease obviously average DNA content and nuclear area, reduce CEA secretion and condensing of the cells, and change LDH isozymoraphy of SGC-7901 cell with promoting H-type LDH and reducing M-type LDH. The change of morphology under light microscope by R II showed that the cells spread, flattened and partially lined up, and the alkalophilic quality of cytoplasm became weak. These results suggest that SGC-7901 cell under the action of R II should undergo the changes of reverse differentiation in morphological, physiological and bio-chemical aspects.
Chen
J
J
Department of Medical Biology and Cell Biology, Chengdu.
Yang
F
F
D000970:Antineoplastic Agents / Q000494:pharmacology*; D002272:Carcinoembryonic Antigen / Q000378:metabolism; D002471:Cell Transformation, Neoplastic / Q000187:drug effects*; D004273:DNA, Neoplasm / Q000378:metabolism; D006801:Humans; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D013274:Stomach Neoplasms / Q000473:pathology*; D014212:Tretinoin / Q000031:analogs & derivatives* / Q000494:pharmacology; D014407:Tumor Cells, Cultured / Q000187:drug effects
D000970:Antineoplastic Agents; D002272:Carcinoembryonic Antigen; D004273:DNA, Neoplasm; D014212:Tretinoin; C054441:retinamide; D007770:L-Lactate Dehydrogenase