title: PCR-generated cross-over linkers for site-directed mutagenesis.
authors.affiliation: MRC Human Genetics Unit, Western General Hospital, Edinburgh, Scotland, UK.
authors.forename: A C
authors.initials: AC
authors.lastname: Boyd
authors.forename: D J
authors.initials: DJ
authors.lastname: Porteous
mesh_terms: D019005:Cystic Fibrosis Transmembrane Conductance Regulator / Q000235:genetics; D004247:DNA / Q000737:chemistry / Q000235:genetics; D017931:DNA Primers; D018076:DNA, Complementary / Q000235:genetics; D015246:Deoxyribonuclease EcoRI / Q000378:metabolism; D015252:Deoxyribonucleases, Type II Site-Specific / Q000378:metabolism; D004926:Escherichia coli / Q000235:genetics; D005091:Exons; D006801:Humans; D007438:Introns; D016297:Mutagenesis, Site-Directed; D010957:Plasmids / Q000235:genetics; D011092:Polyethylene Glycols; D016133:Polymerase Chain Reaction; D017422:Sequence Analysis, DNA; D017385:Sequence Homology; D014169:Transformation, Bacterial
chemical_list: C505032:CFTR protein, human; D017931:DNA Primers; D018076:DNA, Complementary; D019005:Cystic Fibrosis Transmembrane Conductance Regulator; D011092:Polyethylene Glycols; D004247:DNA; D015246:Deoxyribonuclease EcoRI; C074326:CTTAAG-specific type II deoxyribonucleases; D015252:Deoxyribonucleases, Type II Site-Specific