TLC resolution of enantiomers of amino acids and dansyl derivatives using (1R,3R,5R)-2-azabicyclo[3,3,0]octan-3-carboxylic acid as impregnating reagent.
The enantiomeric resolution of certain amino acids and some of their dansyl derivatives was achieved on thin silica gel plates impregnated with (1R,3R,5R)- 2-Azabicyclo[3,3,0]octan-3-carboxylic acid, which is an industrial waste material, and a proline analogue non proteinogenic alpha-amino acid. Different combination of 0.5 M NaCl-MeCN were found to be successful in resolving dansyl-DL-amino acids; addition of methanol was required in some cases. Spots were visualised using a fixed wavelength (254 nm) ultraviolet chamber. Resolution of DL-amino acids was successful in different combinations of MeCN-MeOH-H2O and detection was made with ninhydrin (0.2% in acetone).
Bhushan
R
R
Department of Chemistry, University of Roorkee, India.
Martens
J
J
Wallbaum
S
S
Joshi
S
S
Parshad
V
V
D000596:Amino Acids / Q000737:chemistry / Q000302:isolation & purification*; D019086:Bridged Bicyclo Compounds, Heterocyclic; D055598:Chemical Phenomena; D002627:Chemistry, Physical; D002855:Chromatography, Thin Layer / Q000379:methods*; D003619:Dansyl Compounds / Q000302:isolation & purification*; D007202:Indicators and Reagents; D013237:Stereoisomerism
D000596:Amino Acids; D019086:Bridged Bicyclo Compounds, Heterocyclic; D003619:Dansyl Compounds; D007202:Indicators and Reagents; C108690:2-azabicyclo(3.3.0)octane-3-carboxylic acid