title: Assessment of dot-blot ELISA sensitivity on membrane sorbent using various peroxidase substrates.
abstract: The sensitivity of the peroxidase reaction in dot-blot ELISA significantly depends on the substrate. The highest sensitivity is observed using benzidine and diamine-phenol combinations as the substrates due to the reaction of the coupled oxidation (NADI).
authors.affiliation: Institute of Microbiology and Virology, Almaty, Kazakhstan.
authors.forename: A P
authors.initials: AP
authors.lastname: Bogoyavlensky
authors.forename: I E
authors.initials: IE
authors.lastname: Digel
authors.forename: V E
authors.initials: VE
authors.lastname: Berezin
mesh_terms: D000818:Animals; D002642:Chick Embryo; D004797:Enzyme-Linked Immunosorbent Assay / Q000592:standards*; D010084:Oxidation-Reduction; D010544:Peroxidases / Q000378:metabolism*; D012680:Sensitivity and Specificity; D013379:Substrate Specificity
chemical_list: D010544:Peroxidases