title: Alpha- and beta-subunits of a V-type membrane ATPase in a hyperthermophilic sulfur-dependent archaeum, Thermococcus sp. KI.
abstract: The genes encoding alpha- and beta-subunits of a V-type ATPase in a sulfur-dependent hyperthermophilic archaeum, Thermococcus sp. KI, were cloned and sequenced. The deduced amino acid sequences were approximately 60, 50 and 25% identical to those of other archaeal, eukaryotic V-type and E. coli F-type ATPase, respectively. Phylogenetic analysis revealed that Thermococcus ATPase was closely related to that of Thermus, and those of Methanosarcina and Halobacterium.
authors.affiliation: Marine Biotechnology Institute, Shimizu Laboratories, Shizuoka, Japan. mbishim1@super.win.or.jp
authors.forename: T
authors.initials: T
authors.lastname: Iida
authors.forename: S
authors.initials: S
authors.lastname: Kanai
authors.forename: K
authors.initials: K
authors.lastname: Inatomi
authors.forename: Y
authors.initials: Y
authors.lastname: Kamagata
authors.forename: T
authors.initials: T
authors.lastname: Maruyama
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D003001:Cloning, Molecular; D017124:Conserved Sequence / Q000235:genetics; D008969:Molecular Sequence Data; D010802:Phylogeny; D017494:Proton Pumps / Q000737:chemistry; D006180:Proton-Translocating ATPases / Q000737:chemistry* / Q000235:genetics; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D019712:Thermococcus / Q000145:classification / Q000201:enzymology* / Q000235:genetics; D025262:Vacuolar Proton-Translocating ATPases
chemical_list: D017494:Proton Pumps; D025262:Vacuolar Proton-Translocating ATPases; D006180:Proton-Translocating ATPases