In vivo tumor necrosis factor-alpha induction following chlorin e6-photodynamic therapy in Buffalo rats.
Photodynamic therapy may induce in the in vivo conditions the cytokine tumor necrosis factor-alpha in Buffalo rats. The sensitizer, i.e. chlorin e6, in the doses 2.5, 5.0 and 7.5 mg/kg of body weight followed by light treatment with total doses 50, 100, 150, 200 and 250 J/cm2 resulted in the increase of serum levels of the cytokine. The levels of tumor necrosis factor-alpha have been determined at different time points using enzyme-linked immunosorbent assay (ELISA). In control animals these levels did not exceed the mean value of 189 pg/ml, whereas in photodynamically treated rats the levels were almost 3-4 times higher. The entire experiment has been carried out on healthy animals; control, tumor-bearing rats have also been included to the present experiment.
Ziółkowski
P
P
Department of Pathology, Medical University of Wrocław, Poland.
Symonowicz
K
K
Milach
J
J
Zawirska
B
B
Szkudlarek
T
T
D000818:Animals; D002735:Chlorophyllides; D004305:Dose-Response Relationship, Drug; D004307:Dose-Response Relationship, Radiation; D008114:Liver Neoplasms, Experimental / Q000097:blood*; D010778:Photochemotherapy; D011166:Porphyrins / Q000494:pharmacology*; D051381:Rats; D011915:Rats, Inbred BUF; D013997:Time Factors; D014409:Tumor Necrosis Factor-alpha / Q000096:biosynthesis*
D002735:Chlorophyllides; D011166:Porphyrins; D014409:Tumor Necrosis Factor-alpha; C062985:phytochlorin