A desalting approach for MALDI-MS using on-probe hydrophobic self-assembled monolayers.
One of the problems encountered in preparing samples for matrix-assisted laser desorption/ionization (MALDI) analysis is the presence of nonvolatile salts in the sample. This difficulty is often exacerbated by the necessity to prepare the sample in the appropriate sample-to-matrix ratio. This paper reports a probe surface derivatization method that greatly simplifies this sample preparation process. By constructing self-assembled monolayers of octadecyl mercaptan (C18) on the MALDI probe surface, we were able to generate a surface capable of reversibly binding polypeptides via hydrophobic interactions, which in turn, permits the analyte to be easily concentrated and desalted directly on the probe tip.
Brockman
A H
AH
Department of Biochemistry and Molecular Biology, University of Georgia, Athens 30602-4712, USA.
Dodd
B S
BS
Orlando
R
R
D002614:Chelating Agents; D010455:Peptides / Q000032:analysis*; D012492:Salts; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods*; D013438:Sulfhydryl Compounds; D013499:Surface Properties
D002614:Chelating Agents; D010455:Peptides; D012492:Salts; D013438:Sulfhydryl Compounds; C034610:octadecyl mercaptoacetate