[Specific binding of human bone morphogenetic protein (2A) with mouse osteoblastic cells].
Human bone morphogenetic protein 2A (hBMP2A) cDNA terminal S67 nucleotides were cloned and expressed in a phage display vector pCSM21. Human BMP2A C-terminal peptide displayed on the surface of the phage can bind specifically to the surface of mouse osteoblastic cell (MC3T3) membrane. ELISA assay showed a positive signal of the binding by using antibody against M13 phage gene 8 protein. After labeling with 3HTdR, the counts of the binding groups were 3 to 10 times higher than the control groups. It suggests that the surface of MC3T3 cells exist the receptor for hBMP2A.
Liu
X
X
Fourth Military Medical University, Institute of Molecular Biology, Xi'an.
Chen
S
S
Chen
N
N
Gao
L
L
Zhao
Z
Z
D000818:Animals; D019485:Bone Morphogenetic Proteins / Q000235:genetics* / Q000378:metabolism; D002462:Cell Membrane / Q000378:metabolism; D002478:Cells, Cultured; D003001:Cloning, Molecular; D004926:Escherichia coli / Q000235:genetics*; D006801:Humans; D051379:Mice; D010006:Osteoblasts / Q000166:cytology / Q000378:metabolism*; D011993:Recombinant Fusion Proteins / Q000378:metabolism*; D015398:Signal Transduction
D019485:Bone Morphogenetic Proteins; D011993:Recombinant Fusion Proteins