Antigen incorporation into liposomes results in the enhancement of IL-4 and IgG1 secretion: evidence for preferential expansion of Th-2 cells.
Liposomes have been used to modify the immunological behaviour of a number of antigens. The present study was designed to evaluate the effect of liposomization of ovalbumin on the induction of Th-1 and Th-2-cell response by monitoring the secretion of lymphokines and IgG Isotypes. Liposomes having varied physicochemical properties (positively and negatively charged, neutral and pH-sensitive) were used for this purpose. Ovalbumin delivered in this way induced preferential secretion of IL-4 and production of antigen-specific IgG1 isotypes. This was observed irrespective of the surface charge properties of the liposomes. Further, the concentration of antigen required for the activation of Th cells was 10(2)- to 10(3)-fold lower after encapsulating it in liposomes. These results suggest that liposomes may prove useful adjuvants to prime Th2-like immune responses.
Agrewala
J N
JN
Institute of Microbial Technology, Chandigarh, India.
Owais
M
M
Gupta
C M
CM
Mishra
G C
GC
D000818:Animals; D000917:Antibody Formation; D000941:Antigens / Q000008:administration & dosage*; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology*; D002460:Cell Line; D002478:Cells, Cultured; D004337:Drug Carriers; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D007074:Immunoglobulin G / Q000096:biosynthesis* / Q000145:classification; D007132:Immunoglobulin Isotypes / Q000096:biosynthesis* / Q000145:classification; D007371:Interferon-gamma / Q000096:biosynthesis; D007375:Interleukin-1 / Q000096:biosynthesis; D007376:Interleukin-2 / Q000096:biosynthesis; D015847:Interleukin-4 / Q000096:biosynthesis*; D008081:Liposomes; D008213:Lymphocyte Activation; D008222:Lymphokines / Q000096:biosynthesis*; D008264:Macrophages / Q000276:immunology; D051379:Mice; D008807:Mice, Inbred BALB C; D010047:Ovalbumin / Q000008:administration & dosage*; D013154:Spleen / Q000276:immunology; D018417:Th1 Cells / Q000276:immunology; D018418:Th2 Cells / Q000276:immunology*
D000941:Antigens; D004337:Drug Carriers; D007074:Immunoglobulin G; D007132:Immunoglobulin Isotypes; D007375:Interleukin-1; D007376:Interleukin-2; D008081:Liposomes; D008222:Lymphokines; D015847:Interleukin-4; D007371:Interferon-gamma; D010047:Ovalbumin