title: Stable DNA-binding yeast vector allowing high-bait expression for use in the two-hybrid system.
authors.affiliation: University of Bordeaux II, France.
authors.forename: O
authors.initials: O
authors.lastname: Louvet
authors.forename: F
authors.initials: F
authors.lastname: Doignon
authors.forename: M
authors.initials: M
authors.lastname: Crouzet
mesh_terms: D019747:Aldose-Ketose Isomerases; D015153:Blotting, Western; D004247:DNA / Q000378:metabolism; D004256:DNA Polymerase I / Q000378:metabolism; D004268:DNA-Binding Proteins / Q000235:genetics*; D015246:Deoxyribonuclease EcoRI / Q000378:metabolism; D015252:Deoxyribonucleases, Type II Site-Specific / Q000378:metabolism; D005656:Fungal Proteins / Q000235:genetics; D015870:Gene Expression; D005822:Genetic Vectors; D006388:Hemagglutinins / Q000235:genetics; D009693:Nucleic Acid Hybridization; D010957:Plasmids; D011993:Recombinant Fusion Proteins; D012441:Saccharomyces cerevisiae / Q000235:genetics*; D029701:Saccharomyces cerevisiae Proteins; D014157:Transcription Factors; D014170:Transformation, Genetic; D001616:beta-Galactosidase / Q000235:genetics
chemical_list: D004268:DNA-Binding Proteins; D005656:Fungal Proteins; C052130:GAL4 protein, S cerevisiae; D006388:Hemagglutinins; D011993:Recombinant Fusion Proteins; D029701:Saccharomyces cerevisiae Proteins; D014157:Transcription Factors; D004247:DNA; D004256:DNA Polymerase I; D015246:Deoxyribonuclease EcoRI; C037106:endodeoxyribonuclease NdeI; D015252:Deoxyribonucleases, Type II Site-Specific; D001616:beta-Galactosidase; D019747:Aldose-Ketose Isomerases; C490353:TRP1 protein, S cerevisiae