Expansion of the pig comparative map by expressed sequence tags (EST) mapping.
We have used a PCR-based approach for the genetical and physical mapping of 34 transcripts isolated from a porcine small intestine cDNA library. All but one gene were regionally localized by using a somatic pig-rodent cell hybrid panel, and 12 genes were mapped by linkage analysis of single-stranded conformational polymorphisms developed in 3' untranslated regions of transcripts. For 20 of the transcripts, the human homolog has already been mapped. This study thus represents a significant contribution to the pig comparative map. Some important findings were that we could clarify the extent of a previously identified inversion event in a region of conserved synteny between SSC6q and HSA1p, that SSC14q does contain a region homologous to HSA1, a situation not clear from earlier ZOO-FISH studies, and that the homology between SSC17 and HSA20 includes the p-arm of HSA20.
Fridolfsson
A K
AK
Department of Animal Breeding and Genetics, Swedish University of Agricultural Sciences, Uppsala, Sweden.
Hori
T
T
Winterø
A K
AK
Fredholm
M
M
Yerle
M
M
Robic
A
A
Andersson
L
L
Ellegren
H
H
D000818:Animals; D002874:Chromosome Mapping / Q000662:veterinary*; D006224:Cricetinae; D003412:Cricetulus; D018076:DNA, Complementary / Q000235:genetics; D015870:Gene Expression; D005819:Genetic Markers; D006822:Hybrid Cells; D051379:Mice; D016133:Polymerase Chain Reaction; D018807:Polymorphism, Single-Stranded Conformational; D013552:Swine / Q000235:genetics*
D018076:DNA, Complementary; D005819:Genetic Markers