Simultaneous determination of creatinine, creatine, and guanidinoacetic acid in human serum and urine using liquid chromatography-atmospheric pressure chemical ionization mass spectrometry.
A rapid and direct method for simultaneous determination of creatinine, creatine, and guanidinoacetic acid in biological samples has been developed by using column liquid chromatography-mass spectrometry (LC/APCI-MS). The determinations of creatinine, creatine, and guanidinoacetic acid in the samples of human urine and serum were carried out by scanning the [M + H]+ ions of each compound. The recoveries of authentic compounds were 93.87 +/- 6.00% (n = 5) for creatinine, 94.80 +/- 8.93% (n = 5) for creatine, and 90. 92 +/- 7.96% (n = 5) for guanidinoacetic acid after ion-exchange resin treatment. The content of creatinine in human urine and serum was also measured by the Jaffé method, a common method of determination of creatinine currently used in hospitals. The contents of creatinine, creatine, and guanidinoacetic acid obtained using LC/APCI-MS coincided well with those reported in previous papers.
Yasuda
M
M
Department of Urology, Kochi Medical School, Kochi, 783, Japan.
Sugahara
K
K
Zhang
J
J
Ageta
T
T
Nakayama
K
K
Shuin
T
T
Kodama
H
H
D001274:Atmospheric Pressure; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D003401:Creatine / Q000097:blood* / Q000652:urine*; D003404:Creatinine / Q000097:blood* / Q000652:urine*; D005998:Glycine / Q000031:analogs & derivatives* / Q000097:blood / Q000652:urine; D006801:Humans; D013058:Mass Spectrometry / Q000379:methods; D013997:Time Factors
D003404:Creatinine; C004946:glycocyamine; D003401:Creatine; D005998:Glycine