Isolation and characterization of dolichol-linked oligosaccharides from Haloferax volcanii.
Biosynthesis of procaryotic glycoproteins has been studied in some detail only in the extreme halophile Halobacterium halobium. To extend these studies for a moderate halophile, dolichol phosphate-linked oligosaccharides were isolated and characterized from Haloferax volcanii. Mannosyl (beta 1-->4)galactosyl phosphodolichol could be characterized as a main component by GC/MS permethylation analysis, mass spectrometry and 1H-NMR-spectroscopy. Furthermore, two additional components, present in lower quantities, were partially characterized and identified as a tetrasaccharyl phosphodolichol containing mannose, galactose, and rhamnose in the ratio of 2:1:1 as well as another dihexosyl phosphodolichol. Both the latter compounds contain an additional charged group. All these oligosaccharides were found to be linked to a dolichol consisting of 11 or 12 isoprene units including a saturated omega-terminal isoprene residue.
Kuntz
C
C
Lehrstuhl für Biochemie I, Universität Regensburg, Germany.
Sonnenbichler
J
J
Sonnenbichler
I
I
Sumper
M
M
Zeitler
R
R
D002848:Chromatography, DEAE-Cellulose; D004286:Dolichols / Q000737:chemistry*; D008401:Gas Chromatography-Mass Spectrometry; D019622:Haloferax volcanii / Q000737:chemistry*; D006868:Hydrolysis; D008055:Lipids / Q000737:chemistry; D009682:Magnetic Resonance Spectroscopy; D009844:Oligosaccharides / Q000737:chemistry / Q000302:isolation & purification*; D011522:Protons
D004286:Dolichols; D008055:Lipids; D009844:Oligosaccharides; D011522:Protons