Cloning and functional analysis of rat cyclin D2 promoter: multiple prolactin-responsive elements.
Structure and function of the promoter region of rat cyclin D2 gene were investigated by cloning, sequence analysis and DNA mobility shift assay using nuclear extracts of prolactin-stimulated Nb2 cells. The results show that the expression of rat cyclin D2 gene is not dependent on the TATA-box but is stimulated by multiple transcription factors induced by prolactin at an immediate-early stage of the cell cycle.
Yang
M
M
Department of Biochemistry, Mie University Faculty of Medicine, Japan.
Hosokawa
Y
Y
Hu
Y
Y
Kaneko
S
S
Kaneko
H
H
Tanaka
M
M
Nakashima
K
K
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D001665:Binding Sites / Q000235:genetics; D002460:Cell Line; D003001:Cloning, Molecular; D056742:Cyclin D2; D016213:Cyclins / Q000187:drug effects / Q000235:genetics*; D005786:Gene Expression Regulation / Q000187:drug effects; D008969:Molecular Sequence Data; D011388:Prolactin / Q000494:pharmacology*; D011401:Promoter Regions, Genetic / Q000187:drug effects; D051381:Rats; D014157:Transcription Factors / Q000187:drug effects / Q000235:genetics / Q000378:metabolism; D014158:Transcription, Genetic / Q000187:drug effects
C486197:Ccnd2 protein, rat; D056742:Cyclin D2; D016213:Cyclins; D014157:Transcription Factors; D011388:Prolactin