Specific reg II gene overexpression in the non-obese diabetic mouse pancreas during active diabetogenesis.
The reg gene, previously described in islets of 90% pancreatectomized and nicotinamide-treated rats, has been shown to be expressed in many pharmacological or surgical animal models of beta cell regeneration. We have studied the non-obese diabetic (NOD) mouse, which represents a good model of spontaneous autoimmune diabetes in which regenerative processes have recently been demonstrated. Two reg genes have been described in the mouse genome, both recognized by the human reg cDNA. In a previous work, using the human probe, we have demonstrated a strong correlation between pancreatic reg gene expression and the likelihood of developing diabetes. In the present study, we have examined the respective levels of both mouse reg I and reg II mRNA in the NOD mouse pancreas using their specific cDNA probes. We found that reg II expression was specifically prevalent compared to reg I, irrespective of sex or state of the disease. Reg II mRNA was particularly increased in overtly diabetic female mice and in cyclophosphamide-treated male mice. These data underline the need to study separately the reg genes using specific probes and show that both reg genes are subjected to various regulations, strongly suggesting that their physiological functions may be different.
Baeza
N
N
Groupe de Recherche sur les Glandes Exocrines, Faculté de Médecine, Hôpital de la Timone, Marseille, France.
Sanchez
D
D
Vialettes
B
B
Figarella
C
C
D000818:Animals; D002135:Calcium-Binding Proteins / Q000096:biosynthesis*; D003520:Cyclophosphamide / Q000494:pharmacology; D003922:Diabetes Mellitus, Type 1 / Q000378:metabolism*; D004198:Disease Susceptibility; D005260:Female; D005786:Gene Expression Regulation / Q000187:drug effects; D006801:Humans; D007328:Insulin / Q000096:biosynthesis; D051847:Lithostathine; D008297:Male; D051379:Mice; D016688:Mice, Inbred NOD; D009419:Nerve Tissue Proteins; D010179:Pancreas / Q000187:drug effects / Q000378:metabolism*; D010750:Phosphoproteins / Q000096:biosynthesis; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000096:biosynthesis; D051381:Rats; D012727:Sex Characteristics; D014158:Transcription, Genetic / Q000187:drug effects
D002135:Calcium-Binding Proteins; D007328:Insulin; D051847:Lithostathine; D009419:Nerve Tissue Proteins; D010750:Phosphoproteins; C497617:REG1A protein, human; C497618:REG1B protein, human; D012333:RNA, Messenger; C497615:Reg1 protein, mouse; C497616:Reg1a protein, rat; D003520:Cyclophosphamide