An estrogen inducible 104 kDa chaperone glycoprotein binds ferric iron containing proteins: a possible role in intracellular iron trafficking.
We have previously described an estrogen inducible, intracellular, homodimeric membrane glycoprotein (subunit Mr 104 kDa) which is structurally related to 'chaperone' proteins (Poola, I. and Kiang J.G., J. Biol. Chem. 269 (1994) 21762-21769). In this report we describe a novel finding that the 104 kDa chaperone protein exhibits affinity for iron containing proteins such as transferrins from several species, human lactoferrin and microbial ferric binding protein (FBP). A single ferric ion in the above proteins appears to be sufficient for binding. It also binds to immobilized ferritin. However, it does not exhibit any affinity for apotransferrins, apolactoferrin, apoferritin and apoFBP. This is the first report of a chaperone protein that exhibits affinity for iron containing proteins.
Poola
I
I
Department of Pharmacology and Biochemistry, Howard University School of Medicine, Washington, D.C. 20059, USA.
D000818:Animals; D001052:Apoferritins / Q000378:metabolism; D001059:Apoproteins / Q000378:metabolism*; D030161:Avian Proteins; D001665:Binding Sites; D002352:Carrier Proteins / Q000378:metabolism; D002417:Cattle; D018833:Chaperonins / Q000096:biosynthesis / Q000737:chemistry* / Q000378:metabolism*; D003207:Conalbumin / Q000378:metabolism; D019281:Dimerization; D004967:Estrogens / Q000494:pharmacology; D006801:Humans; D007501:Iron / Q000378:metabolism; D007781:Lactoferrin / Q000378:metabolism; D008562:Membrane Glycoproteins / Q000096:biosynthesis / Q000737:chemistry* / Q000378:metabolism*; D051379:Mice; D011990:Receptors, Transferrin / Q000096:biosynthesis / Q000737:chemistry* / Q000378:metabolism*; D014168:Transferrin / Q000378:metabolism*
D001059:Apoproteins; D030161:Avian Proteins; D002352:Carrier Proteins; D004967:Estrogens; D008562:Membrane Glycoproteins; D011990:Receptors, Transferrin; D014168:Transferrin; C039223:apolactoferrin; C028284:apotransferrin; C088981:estrogen-inducible transferrin receptor-like membrane glycoprotein, chicken; D003207:Conalbumin; D001052:Apoferritins; D007501:Iron; D007781:Lactoferrin; D018833:Chaperonins