title: The use of RT-"nested" PCR of prostate specific antigen to detect hematogenous neoplastic cells in patients with prostate adenocarcinoma.
abstract: The aim of this study was to determine the presence of hematogenous neoplastic cells in patients with prostate cancer. We used a reverse transcription (RT) "nested" polymerase chain reaction (PCR) of prostate-specific antigen (PSA) mRNA to detect the presence of circulating tumor cells in 52 patients who underwent radical prostatectomy with lymphadenectomy. Blood samples were obtained before and after the surgical manipulation. Seven (13.5%) preoperative samples presented evidence of circulating neoplastic cells. All postoperative specimens studied presented a negative result at analysis 24 h after surgical manipulation. Although we did not find a statistical correlation between the PSA-PCR results and clinical-histopathological parameters, the presence of circulating prostate cells was strongly correlated with an elevated Gleason score of primary tumor (P<0.01). Thus our data show the positive effect of surgical treatment in removing the metastases source. The sensitive RT-nested PCR assay may play a crucial role in the administration of adjuvant therapy of patients with prostate adenocarcinoma.
authors.affiliation: Department of Clinical Physiopathology, and Medical Clinics and Cardiology, University of Florence, Italy.
authors.forename: I
authors.initials: I
authors.lastname: Sardi
authors.forename: M
authors.initials: M
authors.lastname: Piazzini
authors.forename: R
authors.initials: R
authors.lastname: Ponchietti
authors.forename: C
authors.initials: C
authors.lastname: Fatini
authors.forename: F
authors.initials: F
authors.lastname: Di Loro
authors.forename: R
authors.initials: R
authors.lastname: Guazzelli
mesh_terms: D000230:Adenocarcinoma / Q000473:pathology* / Q000601:surgery; D000368:Aged; D017931:DNA Primers; D006801:Humans; D008197:Lymph Node Excision; D008198:Lymph Nodes / Q000473:pathology; D008297:Male; D008875:Middle Aged; D009367:Neoplasm Staging; D016133:Polymerase Chain Reaction / Q000379:methods*; D017430:Prostate-Specific Antigen / Q000032:analysis* / Q000096:biosynthesis / Q000097:blood; D011471:Prostatic Neoplasms / Q000473:pathology* / Q000601:surgery; D012333:RNA, Messenger / Q000378:metabolism; D012194:RNA-Directed DNA Polymerase; D014158:Transcription, Genetic
chemical_list: D017931:DNA Primers; D012333:RNA, Messenger; D012194:RNA-Directed DNA Polymerase; D017430:Prostate-Specific Antigen