Prostaglandin F2alpha induces expression of prostaglandin G/H synthase-2 in the ovine corpus luteum: a potential positive feedback loop during luteolysis.
The primary role of prostaglandin (PG) F2alpha in regression of the corpus luteum has been clearly demonstrated in many mammalian species. We have used in vivo and in vitro approaches to investigate the possibility that exogenous PGF2alpha induces expression of prostaglandin G/H synthase-2 (PGHS-2; cyclooxygenase-2) and causes production of PGF2alpha in ovine luteal cells. Ewes received infusions into the ovarian artery of 1 ml PGF2alpha (1 micromol) or saline, and corpora lutea were collected at various times and analyzed for PGHS-2 mRNA using quantitative, competitive reverse transcription polymerase chain reaction. PGF2alpha dramatically increased the steady-state concentration of mRNA for PGHS-2 within 1 h, but basal concentration returned at 12 h posttreatment. In vitro studies using isolated ovine large luteal cells indicated that mRNA for PGHS-2 was induced by PGF2alpha, phorbol didecanoate, and ionomycin in a pattern similar to that observed in vivo. PGHS-2 protein was induced by all three treatments 4-12 h later, and accumulation of PGF2alpha in the culture media increased at 12 and 24 h posttreatment. In conclusion, we have provided evidence that PGF2alpha, probably acting through the protein kinase C/free intracellular calcium pathway, can stimulate large luteal cells to express PGHS-2 and produce PGF2alpha. This luteal PGF2alpha is likely to have an autocrine/paracrine function to augment the luteolytic effect of PGF2alpha of uterine origin.
Tsai
S J
SJ
Dairy Science Department, University of Wisconsin-Madison, 53706, USA.
Wiltbank
M C
MC
D000818:Animals; D015153:Blotting, Western; D002478:Cells, Cultured; D003338:Corpus Luteum / Q000187:drug effects / Q000201:enzymology*; D051546:Cyclooxygenase 2; D015237:Dinoprost / Q000494:pharmacology*; D004790:Enzyme Induction / Q000187:drug effects; D005246:Feedback / Q000187:drug effects / Q000502:physiology; D005260:Female; D007527:Isoenzymes / Q000096:biosynthesis*; D008186:Luteolytic Agents / Q000494:pharmacology*; D016133:Polymerase Chain Reaction; D011451:Prostaglandin-Endoperoxide Synthases / Q000096:biosynthesis*; D012313:RNA / Q000096:biosynthesis / Q000302:isolation & purification; D012756:Sheep; D014158:Transcription, Genetic
D007527:Isoenzymes; D008186:Luteolytic Agents; D012313:RNA; D015237:Dinoprost; D051546:Cyclooxygenase 2; D011451:Prostaglandin-Endoperoxide Synthases