Water channel AQP1, 3, and 4 in the human peritoneum and peritoneal dialysate.
To clarify the mechanism of water transport driven by osmotic gradient through "ultrasmall pores" in the peritoneum, we tried to identify water channels in the peritoneum and cells in the peritoneal dialysate. Peritoneum was surgically excised from uremic patients at the insertion or removal of a catheter. Sediment was collected from 2 L of peritoneal dialysate by centrifugation at 1500 rpm. RNA was extracted and amplified by reverse transcription-polymerase chain reaction (RT-PCR). Contamination of reticulocytes was tested by the presence of ankyrin mRNA. Peritoneal tissue expressed aquaporin (AQP) 1, 3, and 4 (AQP1 > 3 > 4). Sediment of dialysate expressed mRNA of AQP1 and AQP3 (AQP1 > AQP3). The sample did not express ankyrin mRNA, indicating that the AQP1 in the sediment did not originate from reticulocytes. These data indicate that aquaporins are present in the peritoneum and might participate in water transport. Further quantitative analysis of aquaporin messages in the dialysate might clarify the pathogenesis of water removal failure.
Akiba
T
T
Department of Internal Medicine, Tokyo Medical and Dental University, Japan.
Ota
T
T
Fushimi
K
K
Tamura
H
H
Hata
T
T
Sasaki
S
S
Marumo
F
F
D017487:Ankyrins / Q000032:analysis / Q000378:metabolism; D051398:Aquaporin 1; D051400:Aquaporin 3; D051401:Aquaporin 4; D020346:Aquaporins; D001692:Biological Transport; D001789:Blood Group Antigens; D001834:Body Water / Q000378:metabolism*; D015314:Dialysis Solutions / Q000737:chemistry; D006801:Humans; D066298:In Vitro Techniques; D007473:Ion Channels / Q000032:analysis / Q000378:metabolism*; D010530:Peritoneal Dialysis; D010537:Peritoneum / Q000378:metabolism*; D016133:Polymerase Chain Reaction; D012313:RNA / Q000032:analysis; D014511:Uremia / Q000378:metabolism / Q000628:therapy
C496020:AQP1 protein, human; C496027:AQP3 protein, human; C496030:AQP4 protein, human; D017487:Ankyrins; D051401:Aquaporin 4; D020346:Aquaporins; D001789:Blood Group Antigens; D015314:Dialysis Solutions; D007473:Ion Channels; D051398:Aquaporin 1; D051400:Aquaporin 3; D012313:RNA