title: The published DNA sequence of human cytomegalovirus strain AD169 lacks 929 base pairs affecting genes UL42 and UL43.
abstract: Compared with the published DNA sequence (M. S. Chee, et al. Curr. Top. Microbiol. Immunol. 154:125-170, 1990), most isolates of human cytomegalovirus strain AD169 contain an additional 929 bp after nucleotide 54612. This results in a changed reading frame for the 5'-terminal 50 codons of gene UL42 and expansion of gene UL43 (a US22 family member) from 187 (3'-truncated) to 423 (full-length) codons. The UL42 and UL43 gene products are nonessential for growth in culture.
authors.affiliation: MRC Virology Unit, University of Glasgow, United Kingdom. d.dargan@vir.gla.ac.uk
authors.forename: D J
authors.initials: DJ
authors.lastname: Dargan
authors.forename: F E
authors.initials: FE
authors.lastname: Jamieson
authors.forename: J
authors.initials: J
authors.lastname: MacLean
authors.forename: A
authors.initials: A
authors.lastname: Dolan
authors.forename: C
authors.initials: C
authors.lastname: Addison
authors.forename: D J
authors.initials: DJ
authors.lastname: McGeoch
mesh_terms: D000595:Amino Acid Sequence; D001482:Base Composition; D001483:Base Sequence; D003587:Cytomegalovirus / Q000235:genetics* / Q000302:isolation & purification; D004279:DNA, Viral; D015246:Deoxyribonuclease EcoRI / Q000378:metabolism; D017353:Gene Deletion; D005814:Genes, Viral; D016679:Genome, Viral; D006801:Humans; D007700:Kinetics; D008969:Molecular Sequence Data; D015183:Restriction Mapping; D017384:Sequence Deletion; D014764:Viral Proteins / Q000235:genetics*
chemical_list: D004279:DNA, Viral; D014764:Viral Proteins; D015246:Deoxyribonuclease EcoRI