Cloning and sequencing of the gene encoding the outer-membrane protein Tbp1 from Actinobacillus pleuropneumoniae. Expression of Tbp1 and Tbp2.
Some Gram-negative bacteria possess a system for the uptake of iron from host's transferrin consisting of two outer-membrane proteins, namely transferrin-binding proteins 1 and 2. Here we present the cloning and sequencing of the gene encoding the transferrin-binding protein 1 from a serotype 1 isolate of the Gram-negative pathogen Actinobacillus pleuropneumoniae. We have also cloned for expression the Tbp1 and Tbp2 encoding sequences. Both proteins were produced in Escherichia coli and further purified. We also report the deduced Tbp1 amino acid sequence comparison to Tbp1s from related species.
Medrano
A
A
Institut de Biologia Fonamental, Universitat Autònoma de Barcelona, Spain.
Querol
E
E
Daban
M
M
D016977:Actinobacillus pleuropneumoniae / Q000235:genetics / Q000378:metabolism*; D000595:Amino Acid Sequence; D001425:Bacterial Outer Membrane Proteins / Q000096:biosynthesis / Q000737:chemistry; D001483:Base Sequence; D002352:Carrier Proteins / Q000096:biosynthesis* / Q000737:chemistry* / Q000235:genetics; D003001:Cloning, Molecular; D006193:Haemophilus influenzae / Q000235:genetics; D033862:Iron-Binding Proteins; D008969:Molecular Sequence Data; D009345:Neisseria meningitidis / Q000235:genetics; D015183:Restriction Mapping; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid; D033863:Transferrin-Binding Proteins
D001425:Bacterial Outer Membrane Proteins; D002352:Carrier Proteins; D033862:Iron-Binding Proteins; D033863:Transferrin-Binding Proteins