title: TLC resolution of enantiomers of amino acids and dansyl derivatives using (1R,3R,5R)-2-azabicyclo[3,3,0]octan-3-carboxylic acid as impregnating reagent.
abstract: The enantiomeric resolution of certain amino acids and some of their dansyl derivatives was achieved on thin silica gel plates impregnated with (1R,3R,5R)- 2-Azabicyclo[3,3,0]octan-3-carboxylic acid, which is an industrial waste material, and a proline analogue non proteinogenic alpha-amino acid. Different combination of 0.5 M NaCl-MeCN were found to be successful in resolving dansyl-DL-amino acids; addition of methanol was required in some cases. Spots were visualised using a fixed wavelength (254 nm) ultraviolet chamber. Resolution of DL-amino acids was successful in different combinations of MeCN-MeOH-H2O and detection was made with ninhydrin (0.2% in acetone).
authors.affiliation: Department of Chemistry, University of Roorkee, India.
authors.forename: R
authors.initials: R
authors.lastname: Bhushan
authors.forename: J
authors.initials: J
authors.lastname: Martens
authors.forename: S
authors.initials: S
authors.lastname: Wallbaum
authors.forename: S
authors.initials: S
authors.lastname: Joshi
authors.forename: V
authors.initials: V
authors.lastname: Parshad
mesh_terms: D000596:Amino Acids / Q000737:chemistry / Q000302:isolation & purification*; D019086:Bridged Bicyclo Compounds, Heterocyclic; D055598:Chemical Phenomena; D002627:Chemistry, Physical; D002855:Chromatography, Thin Layer / Q000379:methods*; D003619:Dansyl Compounds / Q000302:isolation & purification*; D007202:Indicators and Reagents; D013237:Stereoisomerism
chemical_list: D000596:Amino Acids; D019086:Bridged Bicyclo Compounds, Heterocyclic; D003619:Dansyl Compounds; D007202:Indicators and Reagents; C108690:2-azabicyclo(3.3.0)octane-3-carboxylic acid