The addition of mitogen-activated protein kinase and p34cdc2 kinase substrate peptides inhibits the flagellar motility of demembranated fowl spermatozoa.
The motility of demembranated fowl spermatozoa was vigorous at 30 degrees C, but decreased markedly following the addition of mitogen-activated protein (MAP) kinase or p34cdc2 kinase substrate peptide. Dephosphorylation of approximately 116, 86 and 79-kDa proteins of demembranated spermatozoa was observed after the addition of MAP kinase or p34cdc2 kinase substrate peptide. The activities of MAP kinase and histone H1 kinase of spermatozoa, estimated by measuring the phosphorylation of myelin basic protein and histone H1 as substrates, were 1.22 and 0.29 pmol/min/ mg protein, respectively. Both enzymatic activities of spermatozoa were lower than those of chick brain, but higher than those of chick liver. These results suggest that the phosphorylation of axonemal and/or accessory cytoskeletal proteins mediated by MAP kinase and p34cdc2 kinase may be involved in the regulation of flagellar movement of fowl spermatozoa.
Ashizawa
K
K
Laboratory of Animal Reproduction, Faculty of Agriculture, Miyazaki University, Japan. a0c303u@cc.miyazaki-u.ac.jp
Hashimoto
K
K
Higashio
M
M
Tsuzuki
Y
Y
D000818:Animals; D016203:CDC2 Protein Kinase / Q000494:pharmacology*; D017871:Calcium-Calmodulin-Dependent Protein Kinases / Q000378:metabolism / Q000494:pharmacology*; D002462:Cell Membrane / Q000502:physiology; D002645:Chickens; D008297:Male; D009842:Oligopeptides / Q000494:pharmacology*; D010766:Phosphorylation; D011494:Protein Kinases / Q000378:metabolism; D013078:Sperm Immobilizing Agents / Q000494:pharmacology*; D013081:Sperm Motility / Q000187:drug effects*; D013082:Sperm Tail / Q000187:drug effects / Q000201:enzymology; D013094:Spermatozoa / Q000187:drug effects* / Q000201:enzymology* / Q000502:physiology; D013379:Substrate Specificity
D009842:Oligopeptides; D013078:Sperm Immobilizing Agents; D011494:Protein Kinases; C102333:histone H1 kinase; D017871:Calcium-Calmodulin-Dependent Protein Kinases; D016203:CDC2 Protein Kinase