DNA-probes for the differentiation of Capnocytophaga species.
We designed oligonucleotides to differentiate between the seven currently known Capnocytophaga species. The oligonucleotides were labelled non-radioactively at the 3' end with digoxigenin. The specificity could be demonstrated in a dot-blot hybridization assay by using the type strains, reference strains, and 37 clinical Capnocytophaga isolates as well as 11 representative strains of other taxa as a template. The sensitivity of the assay was calculated with 10(3) bacteria per dot.
Conrads
G
G
Clinic of Conservative & Preventive Dentstry and Periodontology, University Hospital (RWTH) Aachen, Germany.
Mutters
R
R
Seyfarth
I
I
Pelz
K
K
D015373:Bacterial Typing Techniques; D002206:Capnocytophaga / Q000145:classification* / Q000235:genetics; D015342:DNA Probes; D004269:DNA, Bacterial / Q000235:genetics; D004275:DNA, Ribosomal / Q000235:genetics; D004076:Digoxigenin; D009693:Nucleic Acid Hybridization; D009838:Oligodeoxyribonucleotides / Q000737:chemistry; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012680:Sensitivity and Specificity; D013045:Species Specificity
D015342:DNA Probes; D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D009838:Oligodeoxyribonucleotides; D012336:RNA, Ribosomal, 16S; D004076:Digoxigenin