Reduced antibody reactivity to hepatitis C virus antigens in hemodialysis patients coinfected with hepatitis B virus.
Antibody reactivities to hepatitis C virus (HCV) antigens and to synthetic peptides derived from different parts of the HCV genome (core, NS4, and NS5) were evaluated in HCV-infected hemodialysis patients. In the RIBA 3 assay, NS5 was significantly less recognizable by sera of hemodialysis patients compared to other HCV-infected subjects. Among hemodialysis patients, those coinfected with hepatitis B virus (HBV) (positive for hepatitis B surface antigen [HBsAg+]) showed a reduction in reactivity to C33 and C100. Sera of only 23% of the hemodialysis patients (37 of 161) reacted with more than three of eight peptides tested, significantly fewer than the 60% (12 of 20) of the sera of other HCV-infected patients tested (P = 0.001). This immunosuppression was also manifested by a reduced frequency of recognition of additional peptides on follow-up. An even more reduced reactivity was observed among the HBV-coinfected patients (HBsAg+). The low-responder hemodialysis patients were not infected with any particular genotype of HCV, and the same HCV genotypes observed in the whole group of hemodialysis patients (1a, 1b, 2a, and 3a) were found circulating in the low-responder group. Even in this low-responder population, the good performance of two peptides (peptide 716, corresponding to a portion of the core, and peptide 59, corresponding to a portion of NS4) corroborates the immunodominance of the conserved epitopes within these peptides.
Devesa
M
M
Laboratorio de Biología de Virus, Centro de Microbiología y Biología Celular, IVIC, Caracas, Venezuela.
Khudyakov
Y E
YE
Capriles
F
F
Blitz
L
L
Fields
H A
HA
Liprandi
F
F
Pujol
F H
FH
D016679:Genome, Viral; D006509:Hepatitis B / Q000097:blood / Q000150:complications / Q000276:immunology*; D006526:Hepatitis C / Q000097:blood / Q000150:complications / Q000276:immunology*; D018937:Hepatitis C Antibodies / Q000096:biosynthesis* / Q000097:blood; D018936:Hepatitis C Antigens / Q000235:genetics / Q000276:immunology* / Q000494:pharmacology*; D006801:Humans; D016056:Immunodominant Epitopes / Q000276:immunology; D006435:Renal Dialysis / Q000009:adverse effects*; D013997:Time Factors; D017361:Viral Nonstructural Proteins / Q000276:immunology / Q000494:pharmacology*; D012324:RNA-Dependent RNA Polymerase
D018937:Hepatitis C Antibodies; D018936:Hepatitis C Antigens; D016056:Immunodominant Epitopes; D017361:Viral Nonstructural Proteins; C093542:NS4 protein, hepatitis C virus; C087036:NS-5 protein, hepatitis C virus; D012324:RNA-Dependent RNA Polymerase