title: Absence of oxidative stress following paradoxical sleep deprivation in rats.
abstract: Paradoxical sleep deprivation was performed on rats using platform technique to investigate the oxidative process associated with it. Levels of superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx), total glutathione (GSH) and malondialdehyde production were measured in brain of rats under control conditions (C) and those on single large platforms (SLP), multiple large platforms (MLP), single small platforms (SSP) and multiple small platforms (MSP) groups. SOD, CAT and GPx brain activity and malondialdehyde production were not modified by any of the procedures. Brain GSH, however, was significantly reduced in both SSP and SLP groups. These results suggest that paradoxical sleep deprivation per se is not associated with oxidative damage. The observed alterations could be attributed to factors such as immobilization and social isolation present in the single platform techniques.
authors.affiliation: Department of Biochemistry, Universidade de São Paulo, Brazil.
authors.forename: V
authors.initials: V
authors.lastname: D'Almeida
authors.forename: D C
authors.initials: DC
authors.lastname: Hipólide
authors.forename: L A
authors.initials: LA
authors.lastname: Azzalis
authors.forename: L L
authors.initials: LL
authors.lastname: Lobo
authors.forename: V B
authors.initials: VB
authors.lastname: Junqueira
authors.forename: S
authors.initials: S
authors.lastname: Tufik
mesh_terms: D000818:Animals; D001921:Brain / Q000378:metabolism; D002374:Catalase / Q000378:metabolism; D005978:Glutathione / Q000378:metabolism; D005979:Glutathione Peroxidase / Q000378:metabolism; D015227:Lipid Peroxidation / Q000502:physiology; D008297:Male; D008315:Malondialdehyde / Q000378:metabolism; D018384:Oxidative Stress / Q000502:physiology*; D051381:Rats; D017208:Rats, Wistar; D012892:Sleep Deprivation / Q000502:physiology*; D012895:Sleep, REM; D013482:Superoxide Dismutase / Q000378:metabolism
chemical_list: D008315:Malondialdehyde; D002374:Catalase; D005979:Glutathione Peroxidase; D013482:Superoxide Dismutase; D005978:Glutathione