Effect of huoxuequyu recipe on erythrocyte ultrastructure and membrane ATPase activity in rats with passive smoking.
The effect of "Huoxuequyu" recipe on erythrocyte membrane Ca(2+)-ATPase activity, Na(+)-K(+)-ATPase activity and erythrocyte ultrastructure in rats exposed to passive smoking were observed. The erythrocyte membrane Ca(2+)-ATPase activity and Na(+)-K(+)-ATPase activity were found to be decreased in the model group as compared with the control group (P < 0.01, P < 0.01), while no significant difference existed between the treated group and the control group (P > 0.05). Our results also showed that there were more deformed red blood cells in model group than in control group under TCM and SEM (P < 0.0001). No significant difference was found between control group and treated group (P > 0.05). Furthermore, these findings indicated that "Huoxuequyu" recipe, a prepared natural herbal medicine, may be involved in modulation of the activity of erythrocyte membrane enzymes, thereby protecting erythrocyte membrane, so as to improve the erythrocyte function.
Chen
Z
Z
Department of Traditional Chinese Medicine, Tongji Hospital, Tongji Medical University, Wuhan.
Jiao
X
X
Yang
F
F
Wu
H
H
Shu
H
H
D000328:Adult; D000818:Animals; D000252:Calcium-Transporting ATPases / Q000097:blood*; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D004910:Erythrocyte Membrane / Q000201:enzymology*; D004912:Erythrocytes / Q000648:ultrastructure*; D006801:Humans; D051381:Rats; D017207:Rats, Sprague-Dawley; D000254:Sodium-Potassium-Exchanging ATPase / Q000097:blood*; D014028:Tobacco Smoke Pollution / Q000009:adverse effects*
D004365:Drugs, Chinese Herbal; D014028:Tobacco Smoke Pollution; D000252:Calcium-Transporting ATPases; D000254:Sodium-Potassium-Exchanging ATPase