RNA aptamers to the peptidyl transferase inhibitor chloramphenicol.
BACKGROUND
The problem of how macromolecules adopt specific shapes to recognize small molecules in their environment is readily addressed through in vitro selections (the SELEX protocol). RNA-antibiotic interactions are particularly attractive systems for study because they provide an opportunity to expand our understanding of molecular recognition by RNA and to facilitate ribosomal modeling. Specifically, the antibiotic chloramphenicol (Cam) naturally binds bacterial ribosomes in the 'peptidyl transferase loop' of 23S ribosomal RNA to inhibit peptide bond formation.


RESULTS
We identified Cam-binding RNA molecules ('aptamers') from two independent initial random RNA populations. Boundary determinations, ribonuclease S1 sensitivity analyses and the activity of truncated minimal RNAs identified a structural motif that is shared by sequences from both selections. The pseudosymmetric motif consists of a highly conserved central helix of five to six base pairs flanked by A-rich bulges and additional helices. Addition of Cam prior to ribonuclease S1 protected nucleotides in the conserved cores from cleavage. Reselection from a pool of mutated variants of the minimal aptamer further refined the sequence requirements for binding. Finally, we used proton nuclear magnetic resonance (NMR) to establish a 1:1 RNA: Cam stoichiometry of the complex. Both the protection and NMR data both show that Cam stabilizes the active fold of this aptamer.


CONCLUSIONS
There are many different RNA sequences that can bind Cam. The Cam aptamers that we examined have a well-defined secondary structure with a binding pocket that appears to be stabilized by Cam. This RNA motif superficially resembles the Cam-binding site in 23S rRNA, although further work is needed to establish the significance of these similarities.
Burke
D H
DH
Department of Molecular, Cellular and Development Biology, University of Colorado, Boulder, CO 80309-0347, USA. dhburke@beagle.colorado.edu
Hoffman
D C
DC
Brown
A
A
Hansen
M
M
Pardi
A
A
Gold
L
L
D001483:Base Sequence; D002701:Chloramphenicol / Q000494:pharmacology*; D004791:Enzyme Inhibitors / Q000737:chemistry* / Q000378:metabolism; D007700:Kinetics; D009682:Magnetic Resonance Spectroscopy; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation; D010458:Peptidyl Transferases / Q000037:antagonists & inhibitors*; D012313:RNA / Q000737:chemistry* / Q000378:metabolism; D012338:RNA, Ribosomal, 23S / Q000378:metabolism
D004791:Enzyme Inhibitors; D012338:RNA, Ribosomal, 23S; D012313:RNA; D002701:Chloramphenicol; D010458:Peptidyl Transferases