title: The efficient catabolism of thrombin-protease nexin 1 complexes is a synergistic mechanism that requires both the LDL receptor-related protein and cell surface heparins.
abstract: Protease nexin 1 (PN1) is a serine protease inhibitor (SERPIN) that acts as a suicide substrate for thrombin (Th) and urokinase-type plasminogen activator (uPA). PN1 forms 1:1 stoichiometric complexes with these proteases, which are then rapidly bound, internalized, and degraded. The low density lipoprotein receptor-related protein (LRP) is the receptor responsible for the internalization of protease-PN1 complexes. However, we found that the LRP is not significantly involved in the initial cell surface binding of thrombin-PN1, leading us to investigate what cellular component was responsible for this initial interaction. Since Th-PN1 complexes retain a high-affinity for heparin after complex formation, unlike several of the other SERPINs, we tested the possibility that cell surface heparins were involved in initial complex binding. Soluble heparin was found to be a potent inhibitor of the binding of Th-PN1 to the cell surface and greatly facilitated the dissociation of Th-PN1 complexes pre-bound in the absence of soluble heparin. To ascertain the role of cell surface heparins, further studies were done using complexes of thrombin and PN1(K7E), a variant of PN1 in which the heparin binding site was rendered non-functional. When added at equal initial concentrations of complexes, Th-PN1(K7E) was catabolized 5- to 10-fold less efficiently than Th-PN1, a direct result of the greatly diminished initial binding of the Th-PN1(K7E) complexes. These data demonstrate the sizable contribution of cell surface heparins to Thrombin-PN1 complex binding and support a model in which these heparins act to concentrate the complexes at the cell surface facilitating their subsequent LRP-dependent endocytosis.
authors.affiliation: Department of Developmental and Cell Biology, School of Biological Sciences, University of California, Irvine, California 92697, USA. mfknauer@uci.edu
authors.forename: M F
authors.initials: MF
authors.lastname: Knauer
authors.forename: S J
authors.initials: SJ
authors.lastname: Kridel
authors.forename: S B
authors.initials: SB
authors.lastname: Hawley
authors.forename: D J
authors.initials: DJ
authors.lastname: Knauer
mesh_terms: D016564:Amyloid beta-Protein Precursor; D002352:Carrier Proteins / Q000378:metabolism*; D002462:Cell Membrane / Q000378:metabolism; D002478:Cells, Cultured; D004705:Endocytosis; D005982:Glutathione Transferase / Q000378:metabolism; D006493:Heparin / Q000378:metabolism*; D006801:Humans; D007457:Iodine Radioisotopes; D007700:Kinetics; D026503:Low Density Lipoprotein Receptor-Related Protein-1; D058255:Protease Nexins; D011485:Protein Binding; D011956:Receptors, Cell Surface; D011971:Receptors, Immunologic / Q000378:metabolism*; D011993:Recombinant Fusion Proteins / Q000378:metabolism; D058258:Serpin E2; D013917:Thrombin / Q000378:metabolism*
chemical_list: D016564:Amyloid beta-Protein Precursor; D002352:Carrier Proteins; D007457:Iodine Radioisotopes; D026503:Low Density Lipoprotein Receptor-Related Protein-1; D058255:Protease Nexins; D011956:Receptors, Cell Surface; D011971:Receptors, Immunologic; D011993:Recombinant Fusion Proteins; C545486:SERPINE2 protein, human; D058258:Serpin E2; D006493:Heparin; C103332:GST-RAP protein, recombinant; D005982:Glutathione Transferase; D013917:Thrombin