title: Effect of ondansetron on Salmonella typhimurium-induced net fluid accumulation in the pig jejunum in vivo.
abstract: Two major pathophysiological mechanisms explaining the diarrhoea induced by Salmonella typhimurium have been suggested to be: (a) invasion of the intestine by the bacteria, and (b) an enterotoxin resembling Vibrio cholerae toxin. Cholera toxin is a potent secretagogue in pig small intestine and induces secretion partly by activating 5-hydroxytryptamine receptors, following release of 5-hydroxytryptamine. Ondansetron is a selective 5-hydroxytryptamine-3 receptor antagonist, which reduces the cholera toxin-evoked fluid accumulation in pig jejunum. The aim of this study was to investigate the effect of ondansetron on Salmonella typhimurium-induced fluid accumulation in ligated loops of pig jejunum in vivo. 10(10) colony-forming units of the bacteria was injected into loops and incubated for 8 hr. 200 mg x kg-1 ondansetron given subcutaneously reduced the Salmonella typhimurium-induced fluid accumulation by about 40%. This results suggests the involvement of 5-hydroxytryptamine and 5-hydroxytryptamine-3 receptors in Salmonella typhimurium-induced diarrhoea.
authors.affiliation: Department of Physiology and Anatomy, Royal Veterinary and Agricultural University, Frederiksberg, Denmark.
authors.forename: G M
authors.initials: GM
authors.lastname: Jensen
authors.forename: M L
authors.initials: ML
authors.lastname: Grøndahl
authors.forename: C G
authors.initials: CG
authors.lastname: Nielsen
authors.forename: E
authors.initials: E
authors.lastname: Skadhauge
authors.forename: J E
authors.initials: JE
authors.lastname: Olsen
authors.forename: M B
authors.initials: MB
authors.lastname: Hansen
mesh_terms: D000818:Animals; D001826:Body Fluids / Q000378:metabolism*; D005260:Female; D066298:In Vitro Techniques; D007583:Jejunum / Q000187:drug effects* / Q000378:metabolism; D017294:Ondansetron / Q000494:pharmacology*; D012486:Salmonella typhimurium / Q000502:physiology*; D013552:Swine / Q000378:metabolism*
chemical_list: D017294:Ondansetron