title: Protection against verocytotoxin in mice induced by liposome-coupled verocytotoxin.
abstract: Purified verocytotoxins (VTs), VT1 and VT2, were coupled to liposomes via glutaraldehyde. During the coupling procedure, both VT1 and VT2 were detoxified. Intraperitoneal injection in BALB/c mice with either VT1-liposome or VT2-liposome induced a substantial amount of anti-VT1 or anti-VT2 IgG antibody production, respectively. Mice immunized with VT2-liposome were protected against intravenous challenge with a lethal dose of VT2 and the degree of protection correlated well with the amount of IgG induced against VT2. Although VT1-liposome failed to induce protection against VT1, the decrease of the body weight observed after the toxin challenge correlated inversely with the amount of anti-VT1 IgG induced, suggesting that VT1 neutralizing antibody was present in VT1-liposome-immune mice. In addition, VT-liposome conjugate induced no detectable anti-VT IgE antibody production. These results demonstrate the potential ability of VT-liposome conjugates for the production of VT vaccine which induces protection against VTs.
authors.affiliation: Department of Safety Research on Biologics, National Institute of Infectious Diseases, Musashimurayama, Tokyo, Japan.
authors.forename: S
authors.initials: S
authors.lastname: Naito
authors.forename: A
authors.initials: A
authors.lastname: Horino
authors.forename: T
authors.initials: T
authors.lastname: Komiya
authors.forename: Y
authors.initials: Y
authors.lastname: Fukuda
authors.forename: M
authors.initials: M
authors.lastname: Takahashi
authors.forename: Y
authors.initials: Y
authors.lastname: Ami
authors.forename: Y
authors.initials: Y
authors.lastname: Suzaki
authors.forename: S
authors.initials: S
authors.lastname: Satoh
authors.forename: F
authors.initials: F
authors.lastname: Gondaira
authors.forename: J
authors.initials: J
authors.lastname: Sugiyama
authors.forename: Y
authors.initials: Y
authors.lastname: Nakano
authors.forename: M
authors.initials: M
authors.lastname: Mori
authors.forename: K
authors.initials: K
authors.lastname: Awai
authors.forename: S
authors.initials: S
authors.lastname: Nishinohara
authors.forename: K
authors.initials: K
authors.lastname: Komuro
authors.forename: T
authors.initials: T
authors.lastname: Uchida
mesh_terms: D000818:Animals; D000907:Antibodies, Bacterial / Q000096:biosynthesis; D001427:Bacterial Toxins / Q000276:immunology*; D001428:Bacterial Vaccines / Q000008:administration & dosage; D003603:Cytotoxins / Q000276:immunology*; D004926:Escherichia coli; D004927:Escherichia coli Infections / Q000209:etiology / Q000276:immunology / Q000517:prevention & control*; D005260:Female; D018796:Immunoconjugates / Q000008:administration & dosage*; D007073:Immunoglobulin E / Q000096:biosynthesis; D007074:Immunoglobulin G / Q000096:biosynthesis; D008081:Liposomes / Q000276:immunology*; D051379:Mice; D008807:Mice, Inbred BALB C; D022622:Shiga Toxin 1
chemical_list: D000907:Antibodies, Bacterial; D001427:Bacterial Toxins; D001428:Bacterial Vaccines; D003603:Cytotoxins; D018796:Immunoconjugates; D007074:Immunoglobulin G; D008081:Liposomes; D022622:Shiga Toxin 1; D007073:Immunoglobulin E