Eighteen new polymorphic markers in the multiple endocrine neoplasia type 1 (MEN1) region.
Multiple endocrine neoplasia type 1 (MEN1) is an autosomal dominant disorder in which affected individuals develop tumors primarily in the parathyroids, anterior pituitary, endocrine pancreas, and duodenum. The locus for MEN1 is tightly linked to the marker PYGM on chromosome 11q13, and linkage analysis has previously placed the MEN1 gene within a 2-Mb interval flanked by markers D11S1883 and D11S449. Loss of heterozygosity (LOH) studies in MEN1 and sporadic tumors have helped narrow the location of the gene to a 600-kb interval between PYGM and D11S449. Eighteen new polymerase chain reaction (PCR)-based polymorphic markers were generated for the MEN1 region, with ten mapping to the PYGM-D11S449 interval. These new markers, along with 14 previously known polymorphic markers, were precisely mapped on a 2.8-Mb (D11S480-D11S913) high-density clone contig-based, physical map generated for the MEN1 region.
Manickam
P
P
Laboratory of Gene Transfer, National Human Genome Research Institute, National Institutes of Health, Bethesda, MD 20892-4442, USA.
Guru
S C
SC
Debelenko
L V
LV
Agarwal
S K
SK
Olufemi
S E
SE
Weisemann
J M
JM
Boguski
M S
MS
Crabtree
J S
JS
Wang
Y
Y
Roe
B A
BA
Lubensky
I A
IA
Zhuang
Z
Z
Kester
M B
MB
Burns
A L
AL
Spiegel
A M
AM
Marx
S J
SJ
Liotta
L A
LA
Emmert-Buck
M R
MR
Collins
F S
FS
Chandrasekharappa
S C
SC
D000483:Alleles; D001483:Base Sequence; D002874:Chromosome Mapping; D002880:Chromosomes, Human, Pair 11 / Q000235:genetics; D003360:Cosmids; D017931:DNA Primers / Q000235:genetics; D018900:Dinucleotide Repeats; D005787:Gene Frequency; D008040:Genetic Linkage; D005819:Genetic Markers; D006801:Humans; D019656:Loss of Heterozygosity; D018598:Minisatellite Repeats; D018761:Multiple Endocrine Neoplasia Type 1 / Q000235:genetics*; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic; D016324:Sequence Tagged Sites
D017931:DNA Primers; D005819:Genetic Markers