title: Effects of lecithinized superoxide dismutase on traumatic brain injury in rats.
abstract: Only small amounts of superoxide dismutase (SOD) are present in the extracellular space to scavenge excess amounts of superoxide anions (02-) released after traumatic brain injury (TBI). Experiments were performed in rats with cerebral contusion produced by weight-drop technique. We investigated the effects of exogenous lecithinized SOD (PC-SOD) on accumulation of 02- produced in our model, by measuring the level of SOD activity (using the NBT-reducing method) and the expression of copper, zinc-SOD (Cu, Zn-SOD) mRNA (by Northern blot analysis). As determined by tissue-specific gravity, administration of PC-SOD reduced brain edema in the periphery of the lesion 6 h after contusion. SOD activity increased in the peripheral region at 30 min after contusion, but returned to normal levels at 6 h after TBI. Administration of PC-SOD increased SOD activity up to 6 h after TBI. The expression of Cu, Zn-SOD mRNA increased in the core region, peripheral portion, and contralateral hemisphere up to 6 h after TBI, then was suppressed in all three regions by PC-SOD. Our results confirm the important role of 02- in the development of brain edema after TBI and indicate that PC-SOD diminishes brain edema through a protective effect against 02-.
authors.affiliation: Department of Neurological Surgery, Institute of Molecular and Cellular Medicine, Okayama University Medical School, Japan.
authors.forename: M
authors.initials: M
authors.lastname: Yunoki
authors.forename: M
authors.initials: M
authors.lastname: Kawauchi
authors.forename: N
authors.initials: N
authors.lastname: Ukita
authors.forename: Y
authors.initials: Y
authors.lastname: Noguchi
authors.forename: S
authors.initials: S
authors.lastname: Nishio
authors.forename: Y
authors.initials: Y
authors.lastname: Ono
authors.forename: S
authors.initials: S
authors.lastname: Asari
authors.forename: T
authors.initials: T
authors.lastname: Ohmoto
authors.forename: M
authors.initials: M
authors.lastname: Asanuma
authors.forename: N
authors.initials: N
authors.lastname: Ogawa
mesh_terms: D000818:Animals; D001834:Body Water / Q000378:metabolism; D001930:Brain Injuries / Q000378:metabolism*; D002540:Cerebral Cortex / Q000187:drug effects / Q000378:metabolism; D008297:Male; D010713:Phosphatidylcholines / Q000494:pharmacology*; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D013482:Superoxide Dismutase / Q000235:genetics / Q000378:metabolism / Q000494:pharmacology*
chemical_list: D010713:Phosphatidylcholines; D012333:RNA, Messenger; D013482:Superoxide Dismutase