Glutamic acid decarboxylase gene expression in the dopamine-denervated striatum: effects of intrastriatal fetal nigral transplants or chronic apomorphine treatment.
Glutamic acid decarboxylase mRNA expression was studied in the striatum of rats subjected to a unilateral 6-hydroxydopamine lesion of the nigrostriatal dopamine projection, followed by either intrastriatal transplants of fetal nigral neurons or chronic apomorphine treatment. For in situ hybridization histochemistry, a 35S-labelled cRNA probe selective for the mRNA encoding the 67-kDa isoform of the enzyme (GAD67) was used. The results show that fetal dopaminergic grafts in the dopamine-denervated striatum restore an inhibitory control on GAD67 gene expression in the host neurons and also counteract the up-regulation of GAD67 mRNA levels induced by chronic apomorphine treatment.
Cenci
M A
MA
Wallenberg Neuroscience Centre, University of Lund, Sweden.
Campbell
K
K
Björklund
A
A
D000818:Animals; D001058:Apomorphine / Q000494:pharmacology*; D001345:Autoradiography; D016380:Brain Tissue Transplantation / Q000502:physiology*; D003342:Corpus Striatum / Q000201:enzymology*; D003714:Denervation; D004298:Dopamine / Q000502:physiology*; D005260:Female; D016332:Fetal Tissue Transplantation / Q000502:physiology; D005968:Glutamate Decarboxylase / Q000096:biosynthesis*; D007527:Isoenzymes / Q000096:biosynthesis; D009474:Neurons / Q000502:physiology / Q000637:transplantation; D016627:Oxidopamine; D012333:RNA, Messenger / Q000096:biosynthesis; D051381:Rats; D017207:Rats, Sprague-Dawley; D013378:Substantia Nigra / Q000502:physiology / Q000637:transplantation*; D013462:Sulfur Radioisotopes; D014158:Transcription, Genetic / Q000187:drug effects / Q000502:physiology*
D007527:Isoenzymes; D012333:RNA, Messenger; D013462:Sulfur Radioisotopes; D016627:Oxidopamine; D005968:Glutamate Decarboxylase; D001058:Apomorphine; D004298:Dopamine