Effect of single point mutations of the human tachykinin NK1 receptor on antagonist affinity.
Molecular modelling and site-directed mutagenesis were used to identify eleven amino acid residues which may be involved in antagonist binding of the human tachykinin NK1 receptor. Recombinant receptors were expressed in mammalian cells using the Semliki Forest virus system. Wild type and mutant receptors showed similar expression levels in BHK and CHO cells, verified by metabolic labelling. Binding affinities were determined for a variety of tachykinin NK1 receptor antagonists in SFV-infected CHO cells. The binding affinity for GR203040, CP 99,994 and CP 96,345 was significantly reduced by mutant Q165A. The mutant F268A significantly reduced the affinity for GR203040 and CP 99,994 and the mutant H197A had reduced affinity for CP 96,345. All antagonists seemed to bind in a similar region of the receptor, but do not all rely on the same binding site interactions. Functional coupling to G-proteins was assayed by intracellular Ca2+ release in SFV-infected CHO cells. The wild type receptor and all mutants except A162L and F268A responded to substance P stimulation.
Lundstrom
K
K
Glaxo Wellcome, Medicines Research Centre, Stevenage, Herts, United Kingdom. kenneth.lundstrom@roche.com
Hawcock
A B
AB
Vargas
A
A
Ward
P
P
Thomas
P
P
Naylor
A
A
D000818:Animals; D001713:Biphenyl Compounds / Q000378:metabolism; D016466:CHO Cells; D002118:Calcium / Q000378:metabolism; D003001:Cloning, Molecular; D006224:Cricetinae; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism; D005260:Female; D005456:Fluorescent Dyes; D016257:Fura-2; D006801:Humans; D016297:Mutagenesis, Site-Directed; D064729:Neurokinin-1 Receptor Antagonists; D010957:Plasmids / Q000235:genetics; D017354:Point Mutation; D011869:Radioligand Assay; D018040:Receptors, Neurokinin-1 / Q000235:genetics* / Q000378:metabolism; D012672:Semliki forest virus / Q000235:genetics; D013373:Substance P / Q000037:antagonists & inhibitors
D001713:Biphenyl Compounds; D005456:Fluorescent Dyes; D064729:Neurokinin-1 Receptor Antagonists; D018040:Receptors, Neurokinin-1; D013373:Substance P; D002118:Calcium; D016257:Fura-2; C066892:CP 96345