Simultaneous HPLC determination of metabolites of the inflammatory cascade.
OBJECTIVE
A model approach is presented to the in vivo inflammation cascade, in which the activities of key enzymes (phospholipase A2 [3.1.1.4], prostaglandin synthase [EC 1.14.99.1], and lipoxygenases [EC 1.13.11.12]) are determined simultaneously in a single in vitro assay.


RESULTS
Detection of phospholipids (up to 50 pM) and arachidonic acid (up to 33 pM) is attained with high sensitivity and without radiolabelling using a SEDERE light scattering detector.


CONCLUSIONS
Thus, in combination with a diode array UV-detector, lipids, prostaglandins, HETEs and other metabolites of the inflammation cascade can be determined with high efficiency using a reversed phase-high performance liquid chromatograph equipped with two highly sensitive detectors in series.
Schepky
A G
AG
Beiersdorf AG, Paul Gerson Unna-Skin Research Center, Hamburg, Germany.
Siegner
R
R
Diembeck
W
W
D019377:12-Hydroxy-5,8,10,14-eicosatetraenoic Acid / Q000032:analysis; D000818:Animals; D001092:Arachidonate 12-Lipoxygenase / Q000378:metabolism; D016718:Arachidonic Acid / Q000032:analysis; D002851:Chromatography, High Pressure Liquid; D003872:Dermatitis / Q000201:enzymology / Q000378:metabolism*; D008084:Lipoxygenase / Q000378:metabolism*; D008297:Male; D010741:Phospholipases A / Q000378:metabolism*; D054467:Phospholipases A2; D010743:Phospholipids / Q000032:analysis; D044262:Prostaglandin H2; D011451:Prostaglandin-Endoperoxide Synthases / Q000378:metabolism*; D011463:Prostaglandins H / Q000032:analysis; D012756:Sheep; D013552:Swine
D010743:Phospholipids; D011463:Prostaglandins H; D016718:Arachidonic Acid; D044262:Prostaglandin H2; D019377:12-Hydroxy-5,8,10,14-eicosatetraenoic Acid; D008084:Lipoxygenase; D001092:Arachidonate 12-Lipoxygenase; D011451:Prostaglandin-Endoperoxide Synthases; D010741:Phospholipases A; D054467:Phospholipases A2