Effect of L-aspartyl-L-phenylalanine methyl ester on leukotriene biosynthesis in macrophage cells.
Macrophage cells treated with L-aspartyl-L-phenylalanine methyl ester (aspartame) produced leukotrienes and other arachidonic acid metabolites. Leukotriene C4, leukotriene B4, and 15-hydroxyeicosatetraenoic acid were the major metabolites detected. The aspartame-treated macrophage cell cultures produced three times as much arachidonic acid metabolites as did the untreated control cell cultures. Leukotriene C4 was produced in the largest amount by the aspartame-treated cells.
Hardcastle
J E
JE
Department of Chemistry and Physics, Texas Women's University, Denton 76204, USA.
Bruch
R J
RJ
D000818:Animals; D016718:Arachidonic Acid / Q000378:metabolism; D001218:Aspartame / Q000494:pharmacology*; D002250:Carbon Radioisotopes; D002478:Cells, Cultured; D002851:Chromatography, High Pressure Liquid; D006893:Hydroxyeicosatetraenoic Acids / Q000096:biosynthesis; D007975:Leukotriene B4 / Q000096:biosynthesis; D017997:Leukotriene C4 / Q000096:biosynthesis; D015289:Leukotrienes / Q000096:biosynthesis*; D017737:Macrophages, Peritoneal / Q000187:drug effects* / Q000378:metabolism*; D051381:Rats
D002250:Carbon Radioisotopes; D006893:Hydroxyeicosatetraenoic Acids; D015289:Leukotrienes; D007975:Leukotriene B4; D016718:Arachidonic Acid; D017997:Leukotriene C4; C025984:15-hydroxy-5,8,11,13-eicosatetraenoic acid; D001218:Aspartame