title: In vivo platelet thrombus formation in microvessels of spontaneously hypertensive rats.
abstract: Sustained high blood pressure causes functional changes in both vascular endothelial cells and platelets. Therefore, we hypothesized that in vivo platelet thrombus formation would be increased in the cremaster muscle microvessels of rats during genetic hypertension. Experiments were carried out on spontaneously hypertensive rats (SHR) and normotensive Wistar Kyoto rats (WKY) at 12 weeks of age. Fluorescein isothiocyanate tagged to bovine serum albumin (FITC-BSA) was injected intraarterially and 450 to 490 nm light was used to activate the FITC-BSA and induce a thrombus within the vasculature. In vivo television microscopy was used to quantitate thrombus formation and microvascular diameter changes. The time of platelet thrombus initiation and subsequent time of thrombus growth were studied at wall shear rates of approximately 2000 sec(-1) and 270 sec(-1) in third-order arterioles and venules, respectively. In SHR, times for platelet thrombus initiation and vessel occlusion were significantly less in both arterioles and venules, whereas time for platelet thrombus growth following initiation was significantly prolonged. Greater shear rates in arterioles compared to venules decreased platelet adhesion and subsequently decreased the rate of thrombus formation in both WKY and SHR groups. However, the ratio of WKY to SHR platelet thrombus growth (platelet aggregation) time remained similar (0.83 +/- 0.06 in arterioles and 0.79 +/- 0.06 in venules). These results indicate that there is increased thrombus formation during hypertension and that the platelet adhesion processes may be of greater importance than platelet aggregation in producing this increase.
authors.affiliation: Center for Applied Microcirculatory Research, University of Louisville, Kentucky 40292, USA.
authors.forename: D
authors.initials: D
authors.lastname: Lominadze
authors.forename: I G
authors.initials: IG
authors.lastname: Joshua
authors.forename: D A
authors.initials: DA
authors.lastname: Schuschke
mesh_terms: D000818:Animals; D001760:Bleeding Time; D001792:Blood Platelets / Q000502:physiology*; D001809:Blood Viscosity; D006973:Hypertension / Q000097:blood*; D008297:Male; D008833:Microcirculation; D051381:Rats; D011918:Rats, Inbred SHR; D011921:Rats, Inbred WKY; D013927:Thrombosis / Q000209:etiology*