title: Light induces peroxidation in retina by activating prostaglandin G/H synthase.
abstract: Prostaglandin G/H synthase (PGHS) has been shown to generate peroxides to a significant extent in the retina and absorbs light at the lower end of the visible spectrum. We postulated that PGHS could be an important initial source of peroxidation in the retina exposed to light, which would in turn alter retinal function. Exposure of pig eyes (in vivo) to light (350 fc/3770 lx) caused after 3 h a 50% increase and by 5 h a 30% decrease in a- and b-wave amplitudes of the electroretinogram (ERG) which were comparable at 380-650 nm and 380-440 nm but were not observed at wavelengths > 450 nm. These effects of light were prevented by free radical scavengers (dimethylthiourea and high-dose allopurinol) and PGHS inhibitors (naproxen and diclofenac), but stable analogs of prostaglandins did not affect the ERG. Both increases and subsequent decreases in ERG wave amplitudes following light exposure in vivo were associated with increases in retinal prostaglandin and malondialdehyde (peroxidation product) levels, which were inhibited by the nonselective PGHS blockers, naproxen and diclofenac. Similar observations were made in vitro on isolated porcine eyecups as well as on retinal membranes exposed to light (250 fc/ 2700 lx) 380-650 nm and 380-440 nm but not at > 500 nm. Both PGHS-1 and PGHS-2 contributed equivalently to light-induced prostaglandin synthesis, as shown after selective PGHS-2 blockers, but mRNA expression of PGHS-1 and 2 was not affected by light. Finally, light stimulated activities of pure PGHS-1 and PGHS-2 isozymes, and these were also shown to produce superoxide radical (detected with fluorogenic spin trap, proxyl fluorescamine). Taken together, data suggest that PGHS- (1 and 2) is activated by short wavelength visible light, and in the retina is an important source of reactive oxygen species which in turn alter retinal electrophysiological function. PGHS thus seems a likely chromophore in setting forth photic-induced retinal injury. Findings provide an explanation for increased sensitivity of the retina to visible light predominantly at the far blue range of its spectrum.
authors.affiliation: Department of Pediatrics, Research Center of Hôpital Stc-Justine, University of Montreal, Canada.
authors.forename: N
authors.initials: N
authors.lastname: Hanna
authors.forename: K G
authors.initials: KG
authors.lastname: Peri
authors.forename: D
authors.initials: D
authors.lastname: Abran
authors.forename: P
authors.initials: P
authors.lastname: Hardy
authors.forename: A
authors.initials: A
authors.lastname: Doke
authors.forename: P
authors.initials: P
authors.lastname: Lachapelle
authors.forename: M S
authors.initials: MS
authors.lastname: Roy
authors.forename: J
authors.initials: J
authors.lastname: Orquin
authors.forename: D R
authors.initials: DR
authors.lastname: Varma
authors.forename: S
authors.initials: S
authors.lastname: Chemtob
mesh_terms: D000818:Animals; D004596:Electroretinography / Q000528:radiation effects; D004789:Enzyme Activation / Q000528:radiation effects; D004795:Enzyme Stability / Q000528:radiation effects; D007527:Isoenzymes / Q000235:genetics / Q000378:metabolism / Q000528:radiation effects; D008027:Light / Q000009:adverse effects*; D015227:Lipid Peroxidation / Q000528:radiation effects; D008315:Malondialdehyde / Q000378:metabolism / Q000528:radiation effects; D008566:Membranes / Q000201:enzymology / Q000528:radiation effects; D010545:Peroxides / Q000009:adverse effects / Q000378:metabolism*; D011451:Prostaglandin-Endoperoxide Synthases / Q000235:genetics / Q000378:metabolism / Q000528:radiation effects*; D011453:Prostaglandins / Q000378:metabolism / Q000528:radiation effects; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics / Q000528:radiation effects; D012160:Retina / Q000201:enzymology* / Q000378:metabolism / Q000528:radiation effects*; D013481:Superoxides / Q000378:metabolism / Q000528:radiation effects; D013552:Swine
chemical_list: D007527:Isoenzymes; D010545:Peroxides; D011453:Prostaglandins; D012333:RNA, Messenger; D013481:Superoxides; D008315:Malondialdehyde; D011451:Prostaglandin-Endoperoxide Synthases