[High efficiency eukaryotic expression of rhIL-12 in COS-7 cells].
In this study, two high-efficiency eukaryotic expression vectors, PED35 and PED40 containing p35 and p40 cDNA of rhIL-12 respectively, were co-transfected into COS-7 cells by modified DEAE-Dextran method. The COS-7 cell culture supernatant had been certified to contain high level of expressed rhIL-12 protein, nearly 600 IU/ml. Furthermore, in the two biological assays, the expressed rhIL-12 protein can cause the proliferation of human PHA-activated lymphoblasts and augmentation of NK cells mediated cytotoxic activity. This study, therefore, could help further exploration of biological activities and potential usage of rhIL-12.
Liu
M
M
Institute of Basic Medical Sciences, CAMS, Beijing.
Zheng
S
S
D000818:Animals; D002455:Cell Division / Q000187:drug effects; D002478:Cells, Cultured; D003602:Cytotoxicity, Immunologic / Q000187:drug effects; D005057:Eukaryotic Cells / Q000378:metabolism; D006801:Humans; D018664:Interleukin-12 / Q000096:biosynthesis* / Q000235:genetics / Q000494:pharmacology; D007694:Killer Cells, Natural / Q000276:immunology; D007963:Leukocytes, Mononuclear / Q000187:drug effects; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000494:pharmacology; D014162:Transfection
D011994:Recombinant Proteins; D018664:Interleukin-12