[Effect of anti-aging drug on DNA methylase of brain in rats].
DNA methylase activity of brain in Wistar rats treated with Shenfang Bushen Shengxue Drug (SBSD) was observed using 3H-labelled methyl group of S-adenosyl-methionine incorporated into DNA. It was found that the SBSD has a marked effect on anti-aging. After SBSD treatment the specific activity of DNA methylase increased, the thermostability and salt-tolerance of it improved slightly, its optional pH changed from 7.5 to 8.0. A and a difference was found between the electrophoresgram of partially purified product of DNA methylase in SBSD treated rats and that in normal rats, suggesting SBSD could change the activity and characteristics of DNA methylase so as to affect the level of DNA methylation, which might be one pathway of SBSD effects on anti-aging.
Bai
J S
JS
Faculty of Biology, Beijing Normal University.
He
Z X
ZX
Wu
Z K
ZK
D000375:Aging / Q000187:drug effects*; D000818:Animals; D001921:Brain / Q000201:enzymology*; D019175:DNA Methylation / Q000187:drug effects; D015254:DNA Modification Methylases / Q000378:metabolism*; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D008297:Male; D051381:Rats; D017208:Rats, Wistar
D004365:Drugs, Chinese Herbal; D015254:DNA Modification Methylases