Site-specific endonucleases RspLKI and RspLKII from Rhodococcus species LK2 are isoschizomers of SphI and BamHI.
Two site-specific endonucleases, RspLKI and RspLKII, have been isolated and purified to functional homogeneity from the soil bacterium Rhodococcus species LK2. RspLKI recognizes the 5'-GCATG decreases C-3' DNA sequence and RspLKII recognizes the 5'-G decreases GATCC-3' sequence (arrows indicate DNA cleavage sites). The isolated enzymes are class II site specific endonucleases and are isoschizomers of endonucleases SphI and BamHI, respectively.
Zabaznaya
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Institute of Protein Research, Russian Academy of Sciences, Pushchino, Moscow Region, Russia. protres@sovam.com
Zheleznaya
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Matvienko
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D002852:Chromatography, Ion Exchange; D004247:DNA / Q000378:metabolism; D015245:Deoxyribonuclease BamHI / Q000302:isolation & purification* / Q000378:metabolism; D015252:Deoxyribonucleases, Type II Site-Specific / Q000302:isolation & purification* / Q000378:metabolism; D004587:Electrophoresis, Agar Gel; D016929:Rhodococcus equi / Q000201:enzymology*; D013379:Substrate Specificity
D004247:DNA; D015245:Deoxyribonuclease BamHI; C046184:endodeoxyribonuclease PaeI; D015252:Deoxyribonucleases, Type II Site-Specific