[Tolerance to rat parathyroid allograft induced by depletion of Ia+ donor cells plus short course cyclosporine].
In this study, Ia+ donor cells of rat parathyroid allografts were depleted by anti-Ia monoclonal antibody plus complement. The secreting function of treated glands as well preserved. Then we transplanted the treated glands into subcapsular of the recipient kindey, and the median survival time (MST) of the allografts were 60 days, compared with 14 days in the fresh PTG group (P < 0.01). If the recipients received 25 mg/kg cyclosponine for three days before transplantation, the MST of about 60% PTG allografts was more than 150 days, with P < 0.01 in comparison with the control group (short course CyA alone, MST: 70 days). These results indicate that long-term survival of rat parathyroid allografts can be achieved by depletion of Ia+ donor cells and short course pretreatment of the recipient with CyA.
Zhang
T P
TP
PUMC Hospital, CAMS, Beijing.
Zhu
Y
Y
Wang
S
S
Guan
H
H
Tang
W
W
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D046508:Culture Techniques; D016572:Cyclosporine / Q000008:administration & dosage*; D006085:Graft Survival / Q000187:drug effects; D000949:Histocompatibility Antigens Class II / Q000276:immunology*; D007165:Immunosuppression Therapy; D007166:Immunosuppressive Agents / Q000008:administration & dosage*; D008297:Male; D010280:Parathyroid Glands / Q000637:transplantation*; D051381:Rats; D017208:Rats, Wistar; D013370:Subrenal Capsule Assay
D000911:Antibodies, Monoclonal; D000949:Histocompatibility Antigens Class II; D007166:Immunosuppressive Agents; D016572:Cyclosporine