Duplication of a region in the multiple cloning site of a plasmid vector to enhance cloning-mediated addition of restriction sites to a DNA fragment.
Cocea
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Institut National de la Santé et de la Recherche Médicale, Faculté de Médecine Necker, Université Descartes, Paris, France.
D001665:Binding Sites; D003001:Cloning, Molecular; D004247:DNA / Q000737:chemistry / Q000378:metabolism*; D004262:DNA Restriction Enzymes / Q000378:metabolism*; D015252:Deoxyribonucleases, Type II Site-Specific / Q000378:metabolism; D005822:Genetic Vectors; D010766:Phosphorylation; D010957:Plasmids
D004247:DNA; D004262:DNA Restriction Enzymes; C047020:endodeoxyribonuclease ScaI; C032824:ATCGAT-specific type II deoxyribonucleases; C025630:CAGCTG-specific type II deoxyribonucleases; C025651:CCCGGG-specific type II deoxyribonucleases; D015252:Deoxyribonucleases, Type II Site-Specific; C067488:GTMKAC-specific type II deoxyribonucleases