title: Diffusibility of selenate, selenite, seleno-methionine, and seleno-cystine during simulated gastrointestinal digestion.
abstract: The present study was undertaken to evaluate the in vitro availability of chemically varying forms of selenium (Se), supplemented in cow's milk. Two inorganic (selenite and selenate) and two organic (seleno-methionine [Se-Met] and seleno-cystine [Se-Cys]) Se sources were evaluated. The in vitro availability was estimated by the diffusibility of Se during simulated gastrointestinal digestion. First, the diffusibility was compared after adding a constant amount of Se as either selenate, selenite, seleno-methionine, or Se-Cys in milk samples. Se-Met and selenate were found to be significantly more diffusible than seleno-cystine and selenite under the simulated gastrointestinal conditions. The tendency for superior in vitro availability of selenate and Se-Met compared to selenite and Se-Cys was confirmed for a supplementation range of 5-40 ng/g of Se. This study suggests that the high diffusibility of selenate and Se-Met in a simulated gastrointestinal environment may contribute to their high absorption in vivo.
authors.affiliation: Department of Pharmaceutical Sciences, University of Antwerp (UIA), Belgium.
authors.forename: L
authors.initials: L
authors.lastname: Shen
authors.forename: K
authors.initials: K
authors.lastname: van Dyck
authors.forename: J
authors.initials: J
authors.lastname: Luten
authors.forename: H
authors.initials: H
authors.lastname: Deelstra
mesh_terms: D000818:Animals; D001682:Biological Availability; D001692:Biological Transport; D019587:Dietary Supplements; D004058:Diffusion; D004063:Digestion / Q000502:physiology*; D005772:Gastrointestinal Transit; D066298:In Vitro Techniques; D007408:Intestinal Absorption; D008892:Milk / Q000378:metabolism; D016566:Organoselenium Compounds / Q000378:metabolism / Q000493:pharmacokinetics*; D064586:Selenic Acid; D018036:Selenium Compounds / Q000378:metabolism / Q000493:pharmacokinetics*; D017279:Selenocysteine / Q000378:metabolism / Q000493:pharmacokinetics; D012645:Selenomethionine / Q000378:metabolism / Q000493:pharmacokinetics; D018038:Sodium Selenite / Q000378:metabolism / Q000493:pharmacokinetics
chemical_list: D016566:Organoselenium Compounds; D018036:Selenium Compounds; D017279:Selenocysteine; D012645:Selenomethionine; D018038:Sodium Selenite; D064586:Selenic Acid