Capillary electrophoretic separation of clomiphene isomers using various cyclodextrins as additives.
The cis (Z) and trans (E) isomers of clomiphene were separated using capillary electrophoresis. Various derivatives of cyclodextrins (CDs) were applied as buffer additives, achieving a resolution of greater than 18. The effect of various parameters, type and concentration of CDs, type and concentration of background electrolytes (BGEs), pH, and organic modifiers has been studied systematically. Migration reversal was observed in several cases. The load ability of the various buffer systems was also taken into account.
Juvancz
Z
Z
EGIS Pharmaceutical Ltd., Department of Pharmacokinetics, Budapest, Hungary.
Urmös
I
I
Klebovich
I
I
D002021:Buffers; D002996:Clomiphene / Q000737:chemistry* / Q000302:isolation & purification*; D003505:Cyclodextrins; D004573:Electrolytes; D019075:Electrophoresis, Capillary / Q000379:methods; D006863:Hydrogen-Ion Concentration; D007202:Indicators and Reagents; D015394:Molecular Structure; D013237:Stereoisomerism
D002021:Buffers; D003505:Cyclodextrins; D004573:Electrolytes; D007202:Indicators and Reagents; D002996:Clomiphene