Tissue specific control regions of the N-methyl-D-aspartate receptor subunit NR2C promoter.
In the mouse brain, the N-methyl-D-aspartate receptor subunit NR2C (epsilon-3) is mainly detected in the cerebellar granule cells starting from the second week of postnatal life. In order to improve our understanding of molecular mechanisms of this neuron-specific, spatial and temporal gene expression, different promoter fragments were used to control indicator genes in nondifferentiated rat pheochromocytoma (PC12) cells, in human embryonal kidney (HEK293) cells and in transgenic mice. A 400 bp NR2C promoter region upstream of the transcriptional start site was identified as a basal promoter that was negatively regulated possibly by a neuron restrictive silencer element (NRSE) that is localized 664 base pairs downstream from the transcriptional start sites.
Suchanek
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B
CNS Research, Troponwerke GmbH & Co. KG, Cologne, Germany.
Seeburg
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PH
Sprengel
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R
D000818:Animals; D001923:Brain Chemistry / Q000235:genetics*; D018507:Gene Expression Regulation, Developmental / Q000502:physiology; D006801:Humans; D008156:Luciferases / Q000378:metabolism; D051379:Mice; D008822:Mice, Transgenic; D016716:PC12 Cells; D011401:Promoter Regions, Genetic / Q000235:genetics*; D051381:Rats; D016194:Receptors, N-Methyl-D-Aspartate / Q000235:genetics*; D014158:Transcription, Genetic; D014407:Tumor Cells, Cultured
D016194:Receptors, N-Methyl-D-Aspartate; D008156:Luciferases