title: 3-Methylaspartate ammonia-lyase as a marker enzyme of the mesaconate pathway for (S)-glutamate fermentation in Enterobacteriaceae.
abstract: The enzyme 3-methylaspartase (3-methylaspartate ammonia-lyase, EC 4. 3.1.2) was found in the cells of enteric bacteria, especially in the genera Citrobacter and Morganella, that were grown under anoxic and oxygen-limited conditions. The enzymes were purified to homogeneity from the cell-free extracts of 18 active strains and had similar enzymological properties such as action on columns, specific activity, molecular weight, subunit structure, and N-terminal amino acid sequence similarity. The production of the enzyme was dependent on the limitation of oxygen during growth and was arrested by aeration. The addition of external electron acceptors such as dimethylsulfoxide could support cell growth and production of the enzyme. Activities of glutamate mutase (EC 5.4.99.1) and (S)-citramalate hydrolyase (EC 4.2.1.34), key enzymes of the mesaconate pathway of (S)-glutamate fermentation in the genus Clostridium, were detected in the cells of the active strains grown under oxygen-limited conditions. Based on the results, the mesaconate pathway is proposed to explain the (S)-glutamate fermentation process observed in Enterobacteriaceae, and 3-methylaspartase could be a marker enzyme for this pathway.
authors.affiliation: Biotechnology Research Center, Faculty of Engineering, Toyama Prefectural University, Japan.
authors.forename: Y
authors.initials: Y
authors.lastname: Kato
authors.forename: Y
authors.initials: Y
authors.lastname: Asano
mesh_terms: D000332:Aerobiosis; D000595:Amino Acid Sequence; D000642:Ammonia-Lyases / Q000096:biosynthesis / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism*; D015415:Biomarkers; D016971:Citrobacter freundii / Q000187:drug effects / Q000201:enzymology / Q000254:growth & development; D004121:Dimethyl Sulfoxide / Q000494:pharmacology; D004579:Electron Transport / Q000187:drug effects; D004755:Enterobacteriaceae / Q000187:drug effects / Q000201:enzymology* / Q000254:growth & development; D005285:Fermentation; D005971:Glutamates / Q000378:metabolism*; D006836:Hydro-Lyases / Q000378:metabolism*; D019751:Intramolecular Transferases / Q000378:metabolism; D007709:Klebsiella / Q000187:drug effects / Q000201:enzymology / Q000254:growth & development; D008969:Molecular Sequence Data; D009566:Nitrates / Q000494:pharmacology; D012977:Sodium Nitrite / Q000494:pharmacology
chemical_list: D015415:Biomarkers; D005971:Glutamates; D009566:Nitrates; C031618:sodium nitrate; D006836:Hydro-Lyases; C021552:(S)-2-methylmalate dehydratase; D000642:Ammonia-Lyases; C034881:methylaspartate ammonia-lyase; D019751:Intramolecular Transferases; C021570:methylaspartate mutase; D012977:Sodium Nitrite; D004121:Dimethyl Sulfoxide