GB virus C infection in patients who underwent liver transplantation.
To elucidate the impact of an infection with the recently discovered GB virus C (GBV-C) on the clinical course after orthotopic liver transplantation (OLT), we studied eight patients who were GBV-C RNA positive after transplantation. Five individuals had been viraemic before transplantation, three became GBV-C RNA positive thereafter. A control group comprised eight patients without pre- or post-transplant GBV-C infection. GBV-C RNA was detected by reverse-transcription followed by nested polymerase-chain-reaction (PCR) with primers corresponding to the NS5 genome region. Nested PCR products were sequenced directly. The five patients infected with GBV-C before transplantation remained GBV-C RNA positive throughout the time of observation. Pre- and post-transplant GBV-C RNA titres were almost identical. Phylogenetic analysis revealed a very close relationship between the pre- and post-transplant viral nucleotide sequences indicating persistent GBV-C infection. No signs of hepatitis could be detected after transplantation in all GBV-C infected patients. However, four out of eight GBV-C RNA positive patients had a clinical course complicated by severe cholestasis, which was not observed in the control group. Although GBV-C infection does not lead to an increase in the rate of post-transplant hepatitis, it might be associated with severe unexplained cholestatic courses after OLT.
Ross
R S
RS
Institute of Virology, Universitätsklinikum Essen, Germany.
Viazov
S
S
Kruppenbacher
J P
JP
Elsner
S
S
Sarr
S
S
Lange
R
R
Eigler
F W
FW
Roggendorf
M
M
D000328:Adult; D002779:Cholestasis / Q000821:virology; D004247:DNA / Q000032:analysis; D005260:Female; D018067:Flaviviridae / Q000235:genetics / Q000302:isolation & purification / Q000472:pathogenicity*; D006525:Hepatitis, Viral, Human / Q000097:blood / Q000235:genetics / Q000821:virology*; D006801:Humans; D008111:Liver Function Tests; D016031:Liver Transplantation; D008297:Male; D008875:Middle Aged; D012367:RNA, Viral / Q000032:analysis
D012367:RNA, Viral; D004247:DNA