[Loss of heterozygosity involving DCC gene in human colorectal cancers is correlated with the metastatic potential].
DCC gene located at chromosome 18q21.3 is an important tumor-supressor gene associated with tumorigenesis and progression, especially in colorectal carcinomas. Adopting Southern blot technique and RFLP analysis, 33 primary Chinese colorectal cancer patients were examined for loss of heterozygosity (LOH) of DCC gene. Among 16 informative cases, 6 (38%) showed allelic deletion. 18q LOH was correlated with lymph node and hepatic metastases (P < 0.05). The results indicate that application of molecular genetics is of value in ascertaining the metastatic potential of colorectal carcinomas.
Gao
S
S
First Teaching Hospital, Department of Surgery, Beijing Medical University.
Xu
W
W
Wan
Y
Y
D000328:Adult; D000368:Aged; D000483:Alleles; D003110:Colonic Neoplasms / Q000235:genetics* / Q000473:pathology; D005260:Female; D017353:Gene Deletion; D016163:Genes, DCC; D006579:Heterozygote; D006801:Humans; D008113:Liver Neoplasms / Q000235:genetics / Q000556:secondary*; D008207:Lymphatic Metastasis / Q000235:genetics; D008297:Male; D008875:Middle Aged; D009362:Neoplasm Metastasis; D012150:Polymorphism, Restriction Fragment Length; D012004:Rectal Neoplasms / Q000235:genetics* / Q000473:pathology