title: Production of superoxide dismutase, beta 2-microglobulin and ubiquitin by human term decidua in vitro.
abstract: In order to study decidual proteins produced during pregnancy, decidual cells were isolated from term placental membranes by collagenase digestion and Percoll gradient centrifugation. Serum-free CMRL-1066 medium, conditioned for 148 h with the purified decidual cells, was collected and concentrated by ultrafiltration and applied to a Sephadex G-50 column. The protein-containing fractions from the column were concentrated, dialyzed, lyophilized, applied to sodium dodecyl sulfate-polyacrylamide gel electrophoresis, and transferred to a polyvinylidene difluoride membrane, followed by sequencing. Superoxide dismutase and two new members of the decidual protein family, beta 2-microglobulin precursor and ubiquitin, were identified by 100% N-terminal sequence homology, and similarities of molecular weights and residue contents.
authors.affiliation: Department of Medicine, Cedars-Sinai Medical Center-UCLA School of Medicine 90048, USA.
authors.forename: S G
authors.initials: SG
authors.lastname: Ren
authors.forename: G D
authors.initials: GD
authors.lastname: Braunstein
mesh_terms: D000595:Amino Acid Sequence; D002478:Cells, Cultured; D006063:Chorionic Gonadotropin / Q000494:pharmacology; D002850:Chromatography, Gel; D017077:Culture Media, Conditioned; D003656:Decidua / Q000378:metabolism*; D004591:Electrophoresis, Polyacrylamide Gel; D005260:Female; D006801:Humans; D008969:Molecular Sequence Data; D011247:Pregnancy; D013482:Superoxide Dismutase / Q000096:biosynthesis* / Q000737:chemistry; D014452:Ubiquitins / Q000096:biosynthesis* / Q000737:chemistry; D001613:beta 2-Microglobulin / Q000096:biosynthesis* / Q000737:chemistry
chemical_list: D006063:Chorionic Gonadotropin; D017077:Culture Media, Conditioned; D014452:Ubiquitins; D001613:beta 2-Microglobulin; D013482:Superoxide Dismutase