title: Developmental changes in integrin beta-subunits in rat cerebral cortex.
abstract: The present study was carried out to characterize the developmental expression of beta 1-, beta 5- and beta 6-integrin subunits in rat cerebral cortex. Using reverse transcriptase-polymerase chain reaction, mRNA of the three beta-subunits were shown to be present at all developmental stages. Based on immunoblots, beta 1-subunit expression was decreased during cortical development from embryonic stages to adults. beta 6-subunit expression appeared only in adult cortex. beta 5-subunit expression did not change during development. These beta-subunits were expressed in cortical embryonic cells as revealed by immunological studies in vitro. beta 5 and beta 6 were also present in neuronal cells and in oligodendrocytes in adult cortex. Altogether, these results demonstrate that rat cerebral cortex expresses distinct integrin beta-subunits with different developmental profiles. This switch of beta-subunits may be an important mechanism for the regulation of cell behaviour during development.
authors.affiliation: SmithKline Beecham, Harlow, Essex, UK.
authors.forename: B
authors.initials: B
authors.lastname: Cousin
authors.forename: C
authors.initials: C
authors.lastname: Leloup
authors.forename: L
authors.initials: L
authors.lastname: Pénicaud
authors.forename: J
authors.initials: J
authors.lastname: Price
mesh_terms: D000818:Animals; D015703:Antigens, CD / Q000378:metabolism*; D001931:Brain Mapping / Q000379:methods*; D002478:Cells, Cultured; D002540:Cerebral Cortex / Q000196:embryology / Q000254:growth & development / Q000378:metabolism*; D005314:Embryonic and Fetal Development / Q000502:physiology; D019012:Integrin beta1 / Q000378:metabolism*; D016133:Polymerase Chain Reaction / Q000379:methods; D051381:Rats; D017208:Rats, Wistar; D014158:Transcription, Genetic
chemical_list: D015703:Antigens, CD; D019012:Integrin beta1