title: Isolation of new bioactive annonaceous acetogenins from Rollinia mucosa guided by liquid chromatography/mass spectrometry.
abstract: Reversed-phase high-performance liquid chromatography (RP-HPLC) fractionation, monitored by liquid chromatography/electrospray mass spectrometry (LC/ESI-MS), led to the isolation of two new bioactive annonaceous acetogenins, rollidecin C (1) and rollidecin D (2), from the bioactive aqueous methanol fraction of the leaves of Rollinia mucosa (Annonaceae). The structures were confirmed by analyses of the 1H and 13C NMR data. In addition, a known adjacent bis-tetrahydrofuran (THF) acetogenin, desacetyluvaricin (3), was isolated from this plant for the first time utilizing the LC/ESI-MS monitoring approach. Compound 1 exhibited selective cytotoxicity toward the colon tumor cell line (HT-29), while 2 showed only borderline cytotoxicity in a panel of six human tumor cell lines.
authors.affiliation: XenoBiotic Laboratories, Inc., Plainsboro, NJ 08536, USA.
authors.forename: Z M
authors.initials: ZM
authors.lastname: Gu
authors.forename: D
authors.initials: D
authors.lastname: Zhou
authors.forename: N J
authors.initials: NJ
authors.lastname: Lewis
authors.forename: J
authors.initials: J
authors.lastname: Wu
authors.forename: G
authors.initials: G
authors.lastname: Shi
authors.forename: J L
authors.initials: JL
authors.lastname: McLaughlin
mesh_terms: D000972:Antineoplastic Agents, Phytogenic / Q000737:chemistry / Q000302:isolation & purification*; D002851:Chromatography, High Pressure Liquid; D005233:Fatty Alcohols / Q000302:isolation & purification*; D005663:Furans / Q000302:isolation & purification*; D006801:Humans; D009682:Magnetic Resonance Spectroscopy; D013058:Mass Spectrometry; D010936:Plant Extracts / Q000302:isolation & purification; D010946:Plants, Medicinal; D014407:Tumor Cells, Cultured / Q000187:drug effects
chemical_list: D000972:Antineoplastic Agents, Phytogenic; D005233:Fatty Alcohols; D005663:Furans; D010936:Plant Extracts; C110002:rollidecin C; C110003:rollidecin D; C046853:desacetyluvaricin