Dexamethasone and interleukin-1 potently synergize to stimulate the production of granulocyte colony-stimulating factor in differentiated THP-1 cells.
The human monocytic leukemic cell line, THP-1, which differentiates toward macrophages in response to phorbol 12-myristate 13-acetate (PMA) was investigated for its ability to produce granulocyte colony-stimulating factor (G-CSF). G-CSF protein was neither produced during PMA-induced differentiation nor in response to dexamethasone (Dex) alone. However, when combined, PMA and Dex synergistically stimulated THP-1 cells to produce G-CSF. The synergistic interaction between PMA and Dex on G-CSF production appeared to be mediated through the production of interleukin-1 (IL-1) since neutralization of IL-1 activity completely inhibited G-CSF production. Further experiments demonstrated that in THP-1 cells pretreated with PMA, Dex potently synergized with IL-1 to stimulate G-CSF production.
Wang
Y
Y
Hipple Cancer Research Center, Dayton, Ohio 45439, USA. wangye@ucbeh.san.uc.edu
Zhang
J J
JJ
Lei
K Y
KY
Pike
J W
JW
D002454:Cell Differentiation; D003907:Dexamethasone / Q000494:pharmacology*; D004357:Drug Synergism; D016179:Granulocyte Colony-Stimulating Factor / Q000096:biosynthesis* / Q000187:drug effects; D006801:Humans; D007223:Infant; D007375:Interleukin-1 / Q000096:biosynthesis / Q000494:pharmacology* / Q000502:physiology; D007948:Leukemia, Monocytic, Acute / Q000378:metabolism* / Q000473:pathology; D008297:Male; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology; D014407:Tumor Cells, Cultured; D014409:Tumor Necrosis Factor-alpha / Q000096:biosynthesis
D007375:Interleukin-1; D014409:Tumor Necrosis Factor-alpha; D016179:Granulocyte Colony-Stimulating Factor; D003907:Dexamethasone; D013755:Tetradecanoylphorbol Acetate