title: Identification of Listeria monocytogenes by colony hybridization test using the virulence-associated hly and inlA genes as probes.
abstract: We developed a method of identification of Listeria monocytogenes based on colony hybridization with nonradioactively labeled DNA probes, represented by the hly and inlA virulence-associated genes. The procedure described in this paper results simple, rapid, specific and reproducible. Since it can be performed in a short time, the above technique can be applied to detect L. monocytogenes from different source and constitutes a noteworthy and alternative tool to identify this gram-positive pathogenic bacterium.
authors.affiliation: Ist. di Microbiologia, Facoltà di Medicina e Chirurgia, Università degli Studi di Roma La Sapienza.
authors.forename: G
authors.initials: G
authors.lastname: Petrone
authors.forename: M
authors.initials: M
authors.lastname: Polidoro
authors.forename: G
authors.initials: G
authors.lastname: Donnarumma
authors.forename: M P
authors.initials: MP
authors.lastname: Conte
authors.forename: E
authors.initials: E
authors.lastname: Papi
authors.forename: L
authors.initials: L
authors.lastname: Seganti
authors.forename: P
authors.initials: P
authors.lastname: Valenti
mesh_terms: D015373:Bacterial Typing Techniques; D015342:DNA Probes / Q000235:genetics*; D005516:Food Microbiology; D005798:Genes, Bacterial / Q000235:genetics*; D008089:Listeria monocytogenes / Q000145:classification* / Q000235:genetics / Q000472:pathogenicity; D014774:Virulence / Q000235:genetics
chemical_list: D015342:DNA Probes