Metabolic cooperation in the selection of thioguanine-resistant mutants does not occur with the human B-lymphoblastoid cell TK6.
Selection for thioguanine resistant mutants in the human B-lymphoblastoid cell TK6 yields the same results in both round and flat bottom 96-well microtiter plates. These results suggest that metabolic cooperation is not an issue in these cells and show that round bottom wells can be used in place of flat bottom wells.
Gundel
R M
RM
Genetics Laboratory, University of Vermont, Burlington 05401, USA.
O'Neill
J P
JP
D001402:B-Lymphocytes; D002460:Cell Line; D002469:Cell Separation / Q000379:methods*; D004351:Drug Resistance; D006801:Humans; D009154:Mutation; D013866:Thioguanine / Q000494:pharmacology*
D013866:Thioguanine