title: Reaction of hypochlorous acid with bovine nasal cartilage comparison to pig articular cartilage.
abstract: The action of sodium hypochlorite (NaOCl) on bovine nasal cartilage was studied by proton nuclear magnetic resonance (1H-NMR) spectroscopy in order to model degradation processes of cartilage caused by neutrophil-derived hypochlorous acid. Nasal cartilage was chosen as a mean of comparison because it differs from articular cartilage in its composition. It contains some more proteoglycans, i.e. polymeric carbohydrates and less collagen than articular cartilage. This is important for studying the influence of hypochlorous acid on cartilage components (collagen and polysaccharides). Cartilage samples were incubated at 37 degrees C with phosphate buffer in the presence or absence of NaOCl. Supernatants were collected and assayed by NMR-spectroscopy. In the presence of pure phosphate buffer, the supernatants of bovine nasal cartilage were less rich in low molecular mass metabolites (e.g. amino acids, lactate) than articular cartilage. However, intense signals for highly mobile N acetyl groups of cartilage polysaccharides were detectable in nasal cartilage. NaOCl caused an increase in signals for acetate and formiate. Signals for N-acetyl groups rose only during the first 25 minutes of incubation with NaOCl. Then, their concentration decreased markedly. These changes were related to an enhanced release of chondroitinsulfate from nasal cartilage.
authors.affiliation: Institut für Medizinische Physik und Biophysik, Medizinische Fakultät, Universität Leipzig, Germany.
authors.forename: J
authors.initials: J
authors.lastname: Schiller
authors.forename: J
authors.initials: J
authors.lastname: Arnhold
authors.forename: A
authors.initials: A
authors.lastname: Zachäus
authors.forename: K
authors.initials: K
authors.lastname: Arnold
mesh_terms: D000818:Animals; D002358:Cartilage, Articular / Q000737:chemistry / Q000187:drug effects*; D002417:Cattle; D006859:Hydrogen; D006997:Hypochlorous Acid / Q000494:pharmacology*; D009682:Magnetic Resonance Spectroscopy; D009300:Nasal Septum / Q000737:chemistry / Q000187:drug effects*; D009666:Nose; D011509:Proteoglycans / Q000032:analysis; D013552:Swine; D013997:Time Factors
chemical_list: D011509:Proteoglycans; D006997:Hypochlorous Acid; D006859:Hydrogen