title: In vitro kill curves of a new semisynthetic echinocandin, LY-303366, against fluconazole-sensitive and -resistant Candida species.
abstract: In vitro killing by a new semisynthetic echinocandin, LY-303366, was characterized using clinical isolates of fluconazole-sensitive (Y58) and -resistant (Y180) Candida albicans as well as Candida glabrata (Y7) and Candida krusei (Y171). The 24-h kill curves for Y58 and Y180 demonstrated dose-independent killing of between 1 and 2 log10 with LY-303366 at concentrations of 0.1, 1, 10, 50, 100, and 1,000 times the MIC. Regrowth did not occur at 24 h with either C. albicans isolate at the aforementioned LY-303366 concentrations. At their MICs, LY-303366 and amphotericin B produced similar killing kinetics in cultures of Y58, Y180, Y7, and Y171, while all cultures exposed to fluconazole at its MIC demonstrated stasis or growth over 24 h.
authors.affiliation: Faculty of Pharmacy, University of Manitoba, Winnipeg, Canada.
authors.forename: J A
authors.initials: JA
authors.lastname: Karlowsky
authors.forename: G A
authors.initials: GA
authors.lastname: Harding
authors.forename: S A
authors.initials: SA
authors.lastname: Zelenitsky
authors.forename: D J
authors.initials: DJ
authors.lastname: Hoban
authors.forename: A
authors.initials: A
authors.lastname: Kabani
authors.forename: T V
authors.initials: TV
authors.lastname: Balko
authors.forename: M
authors.initials: M
authors.lastname: Turik
authors.forename: G G
authors.initials: GG
authors.lastname: Zhanel
mesh_terms: D000077612:Anidulafungin; D000935:Antifungal Agents / Q000494:pharmacology*; D002175:Candida / Q000187:drug effects* / Q000254:growth & development; D004305:Dose-Response Relationship, Drug; D004352:Drug Resistance, Microbial; D054714:Echinocandins; D015725:Fluconazole / Q000494:pharmacology*; D008826:Microbial Sensitivity Tests; D010456:Peptides, Cyclic / Q000494:pharmacology*
chemical_list: D000935:Antifungal Agents; D054714:Echinocandins; D010456:Peptides, Cyclic; D015725:Fluconazole; D000077612:Anidulafungin