title: Electrolyte environment modulates differentiation in embryonic renal collecting duct epithelia.
abstract: The influence of electrolytes on the development of renal principal and intercalated collecting duct cells is unknown. Consequently embryonic collecting duct epithelia were exposed to different electrolyte concentrations, and their degree of differentiation was registered by immunohistochemical methods. Embryonic collecting duct epithelia were isolated from neonatal rabbit kidneys and placed on tissue carriers. The apical urine and the basal serum compartments were simulated in a gradient culture container. The two sides of the epithelium were each constantly superfused with medium for 13 days. In controls the medium on both apical and basal side was standard Iscove's modified Dulbecco's Medium (IMDM) with 112 mmol/l Na+ and 85 mmol/l Cl-. In experimental series the NaCl concentration at the basal side of the epithelium was increased up to 137 mmol/l Na+ and 99 mmol/l Cl- as found in the serum of neonatal rabbits. Light microscopy revealed morphologically faultless epithelia following gradient perfusion culture in standard and NaCl-adapted IMDM. The development of principal and intercalated cell features was monitored with the monoclonal antibodies 703, 503, PCD9, and peanut lectin. Cells immunopositive for monoclonal antibody 703, for example, increased from less than 10% in controls to more than 80% in NaCl-adapted IMDM. It is a new finding that the development of collecting duct cell features is influenced by the extracellular electrolyte environment.
authors.affiliation: Department of Anatomy, University of Regensburg, Germany.
authors.forename: W W
authors.initials: WW
authors.lastname: Minuth
authors.forename: P
authors.initials: P
authors.lastname: Steiner
authors.forename: R
authors.initials: R
authors.lastname: Strehl
authors.forename: S
authors.initials: S
authors.lastname: Kloth
authors.forename: M
authors.initials: M
authors.lastname: Tauc
mesh_terms: D000818:Animals; D002454:Cell Differentiation / Q000187:drug effects*; D002455:Cell Division / Q000187:drug effects; D003470:Culture Media; D046508:Culture Techniques; D004847:Epithelial Cells / Q000166:cytology; D004848:Epithelium / Q000196:embryology; D005942:Gluconates / Q000494:pharmacology; D007150:Immunohistochemistry; D007685:Kidney Tubules, Collecting / Q000166:cytology / Q000196:embryology*; D018021:Lithium Chloride / Q000494:pharmacology; D011189:Potassium Chloride / Q000494:pharmacology; D011817:Rabbits; D012965:Sodium Chloride / Q000494:pharmacology*
chemical_list: D003470:Culture Media; D005942:Gluconates; D012965:Sodium Chloride; D011189:Potassium Chloride; D018021:Lithium Chloride; C030691:gluconic acid