Prostaglandin modulation of mouse and human sperm capacitation.
To determine whether prostaglandins produce a capacitation and/or acrosome reaction, the effect of prostaglandins on capacitated mouse spermatozoa and the effect of prostaglandin pre-incubation on human and mouse spermatozoa were studied. Prostaglandins did not induce an acrosome reaction in capacitated mouse sperm. PGE1 pre-incubation in a protein-free medium enhanced acrosome loss of mouse sperm challenged with A-23187 or solubilized mouse zona pellucida. Human sperm were pre-incubated in media containing prostaglandins, and an acrosome reaction was induced with calcium ionophore or human follicular fluid. PGE1 pre-incubation enhanced acrosome loss by human sperm when the action was induced with calcium ionophore, but had no effect on follicular fluid induction. We conclude that PGE1 acts as a capacitating factor in vitro for mouse spermatozoa, and enhances acrosome-reaction induction with calcium ionophore in human spermatozoa.
Herrero
M B
MB
Centro de Estudios Farmacológicos y Botánicos, Consejo Nacional de Investigaciones Cientificas y Técnicas de la República Argentina.
Viggiano
J M
JM
Boquet
M
M
Gimeno
M A
MA
D000177:Acrosome / Q000187:drug effects / Q000502:physiology; D000527:Alprostadil / Q000494:pharmacology; D000818:Animals; D000001:Calcimycin / Q000494:pharmacology; D015237:Dinoprost / Q000494:pharmacology; D015232:Dinoprostone / Q000494:pharmacology; D005260:Female; D015571:Follicular Fluid; D006801:Humans; D007476:Ionophores / Q000494:pharmacology; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D011453:Prostaglandins / Q000494:pharmacology*; D013075:Sperm Capacitation / Q000187:drug effects*; D013094:Spermatozoa / Q000187:drug effects / Q000502:physiology / Q000648:ultrastructure; D015044:Zona Pellucida
D007476:Ionophores; D011453:Prostaglandins; D000001:Calcimycin; D015237:Dinoprost; D000527:Alprostadil; D015232:Dinoprostone