Propionyl coenzyme A carboxylase is required for development of Myxococcus xanthus.
A dcm-1 mutant, obtained by transposon mutagenesis of Myxococcus xanthus, could aggregate and form mounds but was unable to sporulate under nutrient starvation. A sequence analysis of the site of insertion of the transposon showed that the insertion lies within the 3' end of a 1,572-bp open reading frame (ORF) designated the M. xanthus pccB ORF. The wild-type form of the M. xanthus pccB gene, obtained from a lambdaEMBL library of M. xanthus, shows extensive similarity to a beta subunit of propionyl coenzyme A (CoA) carboxylase, an alpha subunit of methylmalonyl-CoA decarboxylase, and a 12S subunit of transcarboxylase. In enzyme assays, extracts of the dcm-1 mutant were deficient in propionyl-CoA carboxylase activity. This enzyme catalyzes the ATP-dependent carboxylation of propionyl-CoA to yield methylmalonyl-CoA. The methylmalonyl-CoA rescued the dcm-1 mutant fruiting body and spore development. During development, the dcm-1 mutant cells also had reduced levels of long-chain fatty acids (C16 to C18) compared to wild-type cells.
Kimura
Y
Y
Department of Bioresource Science, Faculty of Agriculture, Kagawa University, Japan. kimura@ag.kagawa-u.ac.jp
Sato
R
R
Mimura
K
K
Sato
M
M
D000595:Amino Acid Sequence; D002262:Carboxy-Lyases / Q000235:genetics* / Q000378:metabolism*; D003001:Cloning, Molecular; D004251:DNA Transposable Elements; D004269:DNA, Bacterial / Q000032:analysis / Q000235:genetics; D005227:Fatty Acids / Q000032:analysis; D015723:Gene Library; D043586:Methylmalonyl-CoA Decarboxylase; D008969:Molecular Sequence Data; D016254:Mutagenesis, Insertional; D016941:Myxococcus xanthus / Q000201:enzymology / Q000235:genetics* / Q000254:growth & development*; D016366:Open Reading Frames; D010957:Plasmids; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D013171:Spores, Bacterial / Q000235:genetics / Q000254:growth & development / Q000378:metabolism
D004251:DNA Transposable Elements; D004269:DNA, Bacterial; D005227:Fatty Acids; D002262:Carboxy-Lyases; D043586:Methylmalonyl-CoA Decarboxylase