Non-radioactive in situ hybridization for mRNA with emphasis on the use of oligodeoxynucleotide probes.
Synthetic oligodeoxynucleotides represent attractive tools for mRNA in situ hybridization. In the present review, guidelines for the preparation, labeling and use of such probes are discussed. Emphasis is placed on sensitivity and specificity issues.
Hougaard
D M
DM
Department of Molecular Cell Biology, Statens Serum Institut, Copenhagen, Denmark. hougaard@biobase.dk
Hansen
H
H
Larsson
L I
LI
D000818:Animals; D003902:Detergents; D010450:Endopeptidases / Q000378:metabolism; D006801:Humans; D006851:Hydrochloric Acid; D017403:In Situ Hybridization / Q000379:methods*; D007553:Isotope Labeling; D015345:Oligonucleotide Probes; D012333:RNA, Messenger / Q000032:analysis*; D012997:Solvents; D016610:Tissue Embedding; D016707:Tissue Fixation
D003902:Detergents; D015345:Oligonucleotide Probes; D012333:RNA, Messenger; D012997:Solvents; D010450:Endopeptidases; D006851:Hydrochloric Acid