Definitive chemical evidence for the constitutive ability of Candida albicans serotype A strains to synthesize beta-1,2 linked oligomannosides containing up to 14 mannose residues.
We have previously reported the presence of phosphate bound beta-1,2 linked oligomannosides with unusually high degrees of polymerization (DP > 7) in the mannan of Candida albicans strain VW32. To confirm this observation, we have prepared these oligomannosides from the mannan of C. albicans strain NIH A 207. Gel filtration chromatography and TLC analysis revealed DP up to 14. For both strains, NMR analysis confirmed the exclusive presence of beta-1,2 linkages in the pools of oligomannosides with a DP higher than 6 which presented an average DP of 10.6 (VW32) and 10.4 (NIH A 207). These results are important to consider in relation with the ability of these C. albicans derived oligomannosides to trigger TNFalpha synthesis according to their DP.
Trinel
P A
PA
Unité 42, Institut National de la Santé et de la Recherche Médicale, Domaine du CERTIA, Villeneuve d'Ascq, France.
Lepage
G
G
Jouault
T
T
Strecker
G
G
Poulain
D
D
D002176:Candida albicans / Q000145:classification / Q000378:metabolism*; D002240:Carbohydrate Sequence; D002850:Chromatography, Gel; D002855:Chromatography, Thin Layer; D008351:Mannans / Q000096:biosynthesis / Q000737:chemistry / Q000302:isolation & purification; D008969:Molecular Sequence Data; D019906:Nuclear Magnetic Resonance, Biomolecular; D009844:Oligosaccharides / Q000096:biosynthesis* / Q000737:chemistry* / Q000494:pharmacology; D010748:Phosphopeptides / Q000737:chemistry / Q000302:isolation & purification; D012703:Serotyping; D014409:Tumor Necrosis Factor-alpha / Q000096:biosynthesis
D008351:Mannans; D009844:Oligosaccharides; D010748:Phosphopeptides; D014409:Tumor Necrosis Factor-alpha; C028569:oligomannoside; C072108:phosphopeptidomannan