Changes of cholinesterase activity in the erythrocytes, plasma, diaphragm, liver and various parts of the brain in the rabbit following transfusion of erythrocytes with soman inhibited acetylcholinesterase.
1. The changes of cholinesterase activity in rabbit blood, peripheral tissues and the central nervous system following transfusion of erythrocytes with soman inhibited acetylcholinesterase we were demonstrated. 2. After incubation with soman for 0.5 or 24 h, erythrocytes without acetylcholinesterase activity were injected to intact rabbits and cholinesterase activity in the erythrocytes, plasma, diaphragm, liver and various parts of the brain were evaluated 24 h following blood-transfusion. 3. When erythrocytes were incubated with soman for 24 h, no changes of cholinesterase activity in the rabbit following blood-transfusion were observed with an exception of erythrocyte acetylcholinesterase. 4. When erythrocytes were incubated with soman for 0.5 h, a significant decrease in cholinesterase activity in the erythrocytes, plasma, diaphragm and liver following blood-transfusion was found. These data show that soman is able to release from erythrocytes and inhibit cholinesterase activities not only in vitro but also in vivo although the significant inhibition of cholinesterase activities by soman was only observed in the peripheral compartment.
Kassa
J
J
Purkynĕ Military Medical Academy, Hradec Králové.
Bajgar
J
J
Fusek
J
J
D000110:Acetylcholinesterase / Q000097:blood / Q000378:metabolism*; D000818:Animals; D001921:Brain / Q000201:enzymology; D002091:Butyrylcholinesterase / Q000378:metabolism; D002619:Chemical Warfare Agents / Q000493:pharmacokinetics; D002800:Cholinesterase Inhibitors / Q000494:pharmacology*; D002802:Cholinesterases / Q000378:metabolism*; D003964:Diaphragm / Q000201:enzymology; D017707:Erythrocyte Transfusion; D004912:Erythrocytes / Q000201:enzymology* / Q000378:metabolism; D008099:Liver / Q000201:enzymology; D008297:Male; D011817:Rabbits; D012999:Soman / Q000493:pharmacokinetics / Q000494:pharmacology*
D002619:Chemical Warfare Agents; D002800:Cholinesterase Inhibitors; D012999:Soman; D000110:Acetylcholinesterase; D002091:Butyrylcholinesterase; D002802:Cholinesterases