Two visual pigment opsins, one expressed in the dorsal region and another in the dorsal and ventral regions, of the compound eye of a dragonfly, Sympetrum frequens.
This paper describes the primary structure of two visual pigment opsins (DfRh1 and DfRh2) in the regionalized compound eye of a dragonfly, Sympetrum frequens. The amino acid sequences were deduced from the nucleotide sequences of cDNAs isolated from a cDNA library of the dragonfly retina. The two opsins both consist of 379 amino acids with 81.3% identity. Analysis of hydropathy indicated that the sequences have seven transmembrane domains like those of previously described opsins. Expression analysis using RT-PCR revealed that DfRh1 was present only in the dorsal region whereas DfRh2 was detected in both the dorsal and the ventral regions of the eye.
Arikawa
K
K
Department of Biology, Yokohama City University, Japan.
Ozaki
K
K
Tsuda
T
T
Kitamoto
J
J
Mishina
Y
Y
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D004596:Electroretinography; D005123:Eye / Q000378:metabolism*; D019476:Insect Proteins; D007313:Insecta / Q000378:metabolism* / Q000502:physiology; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D012160:Retina / Q000378:metabolism / Q000502:physiology; D017299:Rod Opsins / Q000235:genetics / Q000378:metabolism*; D014018:Tissue Distribution; D014158:Transcription, Genetic
C109292:DfRh1 protein, Sympetrum frequens; C109293:DfRh2 protein, Sympetrum frequens; D019476:Insect Proteins; D017299:Rod Opsins