title: Nitric oxide enhances hydrogen peroxide-mediated endothelial permeability in vitro.
abstract: The objective of this study was to evaluate the effects of nitric oxide (NO) on H2O2-mediated endothelial permeability. H2O2 (0.1 mM) increased permeability at 90 min to 298% of baseline. Spermine NONOate (SNO), an NO donor, at 0.1 or 1 mM did not alter permeability. However, 0.1 mM H2O2 + 1 mM SNO increased permeability to 764%, twice that of 0.1 mM H2O2 alone. These treatments were not directly toxic to endothelial cells. This NO effect was concentration dependent, inasmuch as 0.1 mM SNO did not significantly change H2O2-mediated permeability. The NO-enhanced, H2O2-dependent permeability required the simultaneous presence of NO and H2O2, inasmuch as preincubation with SNO for 30 min followed by 0.1 mM H2O2 did not alter permeability. Staining of endothelial junctions showed widening of the intercellular space only in junctions of cells exposed to H2O2 (0.1 mM) + SNO (1 mM). Furthermore, NO did not affect H2O2 metabolism by endothelial cells but significantly depleted intracellular glutathione. This reduction of cell glutathione produced by NO exposure recovered 15-30 min after removal of the NO donor. NO-enhanced permeability was completely blocked by methionine (1 mM), a scavenger of reactive oxygen species, and by the iron chelator desferrioxamine (0.1 mM). These results suggest that NO may exacerbate the effects of H2O2-dependent increase in endothelial monolayer permeability via the iron-catalyzed formation of reactive oxygen metabolites.
authors.affiliation: Department of Molecular and Cellular Physiology, Louisiana State University Medical Center, Shreveport 71130-3932, USA.
authors.forename: N
authors.initials: N
authors.lastname: Okayama
authors.forename: C G
authors.initials: CG
authors.lastname: Kevil
authors.forename: L
authors.initials: L
authors.lastname: Correia
authors.forename: D
authors.initials: D
authors.lastname: Jourd'heuil
authors.forename: M
authors.initials: M
authors.lastname: Itoh
authors.forename: M B
authors.initials: MB
authors.lastname: Grisham
authors.forename: J S
authors.initials: JS
authors.lastname: Alexander
mesh_terms: D000818:Animals; D002417:Cattle; D002463:Cell Membrane Permeability / Q000187:drug effects / Q000502:physiology*; D002478:Cells, Cultured; D004357:Drug Synergism; D004730:Endothelium, Vascular / Q000166:cytology / Q000187:drug effects / Q000502:physiology*; D005978:Glutathione / Q000378:metabolism; D006861:Hydrogen Peroxide / Q000494:pharmacology*; D007365:Intercellular Junctions / Q000187:drug effects; D007700:Kinetics; D008715:Methionine / Q000494:pharmacology; D009569:Nitric Oxide / Q000494:pharmacology*; D009589:Nitrogen Oxides; D011651:Pulmonary Artery; D013096:Spermine / Q000031:analogs & derivatives* / Q000494:pharmacology
chemical_list: D009589:Nitrogen Oxides; C091861:spermine nitric oxide complex; D013096:Spermine; D009569:Nitric Oxide; D008715:Methionine; D006861:Hydrogen Peroxide; D005978:Glutathione