title: Development and validation of capillary electrophoresis methods for pharmaceutical dissolution assays.
abstract: The determination of active compounds in samples of dissolution tests of oral solid dosage forms based on the USP 23 methods was performed by capillary electrophoresis after the use of solid-phase extraction disks for the preconcentration of drugs. Enrichment factors of 20:1 allowed the determination of betamethasone and ergotamine tartrate at levels of 0.33 microgram/mL and 1.0 microgram/mL, respectively. CE analysis was performed using fused-silica capillaries (35 or 60 cm length x 75 microns i.d.) and the operating conditions consisted of 15 kV applied voltage and UV detection at 254 nm. The background electrolyte was 20-mM phosphate borate buffer, pH 9.0, containing 50 mM of sodium cholate for the separation of betamethasone and 25 mM phosphate buffer, pH 3.0, for ergotamine tartrate. Validation of the methods was also performed. Accuracy and precision of the intraday and interday assays showed comparable results with those obtained by HPLC.
authors.affiliation: Department of Analytical Chemistry and Physicochemistry, Faculty of Pharmacy and Biochemistry, University of Buenos Aires, Argentina.
authors.forename: S E
authors.initials: SE
authors.lastname: Lucangioli
authors.forename: V G
authors.initials: VG
authors.lastname: Rodríguez
authors.forename: G C
authors.initials: GC
authors.lastname: Fernández Otero
authors.forename: N M
authors.initials: NM
authors.lastname: Vizioli
authors.forename: C N
authors.initials: CN
authors.lastname: Carducci
mesh_terms: D000284:Administration, Oral; D001623:Betamethasone / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D004304:Dosage Forms; D019075:Electrophoresis, Capillary / Q000379:methods*; D004878:Ergotamine / Q000032:analysis*; D007202:Indicators and Reagents; D004364:Pharmaceutical Preparations / Q000737:chemistry*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013056:Spectrophotometry, Ultraviolet / Q000379:methods
chemical_list: D004304:Dosage Forms; D007202:Indicators and Reagents; D004364:Pharmaceutical Preparations; D001623:Betamethasone; D004878:Ergotamine