The herpes simplex virus virulence factor ICP34.5 and the cellular protein MyD116 complex with proliferating cell nuclear antigen through the 63-amino-acid domain conserved in ICP34.5, MyD116, and GADD34.
The herpes simplex virus (HSV) virulence factor ICP34.5, the mouse myeloid differentiation protein MyD116, and the hamster growth arrest and DNA damage protein GADD34 share a 63-amino-acid carboxyl domain which has significant homologies to otherwise divergent proteins. Here we report that both ICP34.5 and its cellular homolog MyD116 complex through the conserved domain with proliferating cell nuclear antigen. In addition, HSV infection induces a novel 70-kDa cellular protein detectable by antisera to both ICP34.5 and GADD34, demonstrating that this novel protein possesses homology with the 63-amino-acid conserved domain.
Brown
S M
SM
Glasgow University Neurovirology Research Laboratories, Institute of Neurological Sciences, Southern General Hospital, United Kingdom. smbrown@clinmed.gla.ac.uk
MacLean
A R
AR
McKie
E A
EA
Harland
J
J
D016475:3T3 Cells; D000595:Amino Acid Sequence; D000818:Animals; D000906:Antibodies / Q000276:immunology; D000943:Antigens, Differentiation; D001665:Binding Sites; D018797:Cell Cycle Proteins; D002460:Cell Line; D017124:Conserved Sequence; D006224:Cricetinae; D004249:DNA Damage; D015870:Gene Expression; D005982:Glutathione Transferase; D018259:Herpesvirus 1, Human / Q000472:pathogenicity; D006801:Humans; D051379:Mice; D009363:Neoplasm Proteins; D018809:Proliferating Cell Nuclear Antigen / Q000378:metabolism*; D054645:Protein Phosphatase 1; D011506:Proteins / Q000235:genetics / Q000276:immunology / Q000378:metabolism*; D012313:RNA; D011817:Rabbits; D011993:Recombinant Fusion Proteins / Q000235:genetics; D014158:Transcription, Genetic; D014407:Tumor Cells, Cultured; D014764:Viral Proteins / Q000235:genetics / Q000276:immunology / Q000378:metabolism*; D014774:Virulence
D000906:Antibodies; D000943:Antigens, Differentiation; D018797:Cell Cycle Proteins; D005982:Glutathione Transferase; D009363:Neoplasm Proteins; D018809:Proliferating Cell Nuclear Antigen; D054645:Protein Phosphatase 1; D011506:Proteins; D012313:RNA; D011993:Recombinant Fusion Proteins; D014764:Viral Proteins; C066469:gamma 34.5 protein, Human herpesvirus 1; C087519:PPP1R15A protein, human; C513256:Ppp1r15a protein, mouse