Cloning and sequencing of the Aa-Pri1 gene specifically expressed during fruiting initiation in the edible mushroom Agrocybe aegerita, and analysis of the predicted amino-acid sequence.
A gene (Aa-Pri1) specifically expressed during fruiting initiation of the basidiomycete Agrocybe aegerita was cloned. The total length of the Aa-Pri1 gene was 492 bp including a class-II intron of 54 bp size located at nt +125; the open reading frame encoded for a 145-aa protein of 16 093 Da. CCAAT (-156) and TATAAAT (- 83) boxes, and T(A)5T(A)2 (+593) and T(A)3T(A)4T(A)6T (+608) putative polyadenylation sequences were identified. The putative transcription start point was located at position 49. The Aa-Pri1 transcript was abundant only during fruiting initiation and was undetectable in the other stages of development. The Aa-Pri1 protein was hydrophilic, with a 20-aa hydrophobic motif in the NH2-terminal part, determining a putative alpha-helix. Two putative glycosylation sites were identified. Aa-Pri1 protein activity may be controlled by the phosphorylation of several residues by different protein kinases.
Fernandez Espinar
M T
MT
Laboratoire de Génétique Moléculaire et d'Amélioration des Champignons Cultivés, INRA - Université Victor Segalen Bordeaux 2, C. R. A. de Bordeaux, BP 81, F-33883 Villenave d'Ornon Cedex, France.
Labarère
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D000251:Adenosine Triphosphatases / Q000235:genetics*; D000363:Agaricales / Q000235:genetics* / Q000254:growth & development; D000595:Amino Acid Sequence; D003001:Cloning, Molecular; D004265:DNA Helicases; D015966:Gene Expression Regulation, Fungal / Q000235:genetics*; D005800:Genes, Fungal / Q000235:genetics; D006031:Glycosylation; D008969:Molecular Sequence Data; D012331:RNA, Fungal / Q000032:analysis; D012333:RNA, Messenger / Q000032:analysis; D012045:Regulatory Sequences, Nucleic Acid; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D014158:Transcription, Genetic / Q000235:genetics
D012331:RNA, Fungal; D012333:RNA, Messenger; D000251:Adenosine Triphosphatases; D004265:DNA Helicases