title: Distribution of metabotropic glutamate receptor type 1a in Purkinje cell dendritic spines is independent of the presence of presynaptic parallel fibers.
abstract: The metabotropic glutamate receptor type 1a (mGluR1a) is expressed at a high level in the molecular layer of the cerebellar cortex, where it is localized mostly in dendritic spines of Purkinje cells, innervated by parallel fibers. Treatment with methylazoxymethanol (MAM) of mouse pups at postnatal days (PND) 0 + 1 or 5 + 6 results in the partial loss of granule cells, the extent of which depends on the age of the animal at the time of injection. As a consequence of hypogranularity, the number of parallel fibers is decreased to such an amount that many of the postsynaptic Purkinje cell dendritic spines are devoid of axonal input, and only a limited number of spines participate in the formation of parallel fiber synapses, or, infrequently, in heterologous or heterotopic synapses with other presynaptic partners. At PND 30, 50% of the spines in the cerebella of mice treated with MAM at PND 0 + 1 was not contacted by any presynaptic element, compared to 5% in controls or 15% in the cerebella of mice treated with MAM at PND 5 + 6. The localization of mGluR1a was visualized by immunocytochemistry on ultrathin sections: approximately 80% of all Purkinje cell dendritic spines were immunopositive in controls and in both groups of MAM-treated mice, indicating that mGluR1a was present in Purkinje dendritic spines even when the corresponding synaptic input was absent. This observation indicates that the expression and subcellular distribution of mGluR1a are inherent, genetically determined properties of Purkinje cells.
authors.affiliation: First Department of Anatomy, Semmelweis Medical University, Budapest, Hungary. takacs@anal.sote.hu
authors.forename: J
authors.initials: J
authors.lastname: Takács
authors.forename: G
authors.initials: G
authors.lastname: Gombos
authors.forename: T
authors.initials: T
authors.lastname: Görcs
authors.forename: T
authors.initials: T
authors.lastname: Becker
authors.forename: J
authors.initials: J
authors.lastname: de Barry
authors.forename: J
authors.initials: J
authors.lastname: Hámori
mesh_terms: D000375:Aging; D000818:Animals; D000831:Animals, Newborn; D001369:Axons / Q000502:physiology / Q000648:ultrastructure; D002525:Cerebellar Cortex / Q000166:cytology / Q000254:growth & development / Q000502:physiology*; D003712:Dendrites / Q000187:drug effects / Q000502:physiology* / Q000648:ultrastructure; D008746:Methylazoxymethanol Acetate / Q000031:analogs & derivatives* / Q000494:pharmacology; D051379:Mice; D008854:Microscopy, Electron; D009412:Nerve Fibers / Q000187:drug effects / Q000502:physiology*; D011689:Purkinje Cells / Q000187:drug effects / Q000502:physiology* / Q000648:ultrastructure; D018094:Receptors, Metabotropic Glutamate / Q000096:biosynthesis*; D013569:Synapses / Q000187:drug effects / Q000502:physiology*; D013723:Teratogens
chemical_list: D018094:Receptors, Metabotropic Glutamate; D013723:Teratogens; C104077:metabotropic glutamate receptor type 1; D008746:Methylazoxymethanol Acetate; C030667:methylazoxymethanol