Differential regulation of D2 receptor gene expression by transcription factor AP-1 in cultured cells.
The role of the transcription factor AP-1 in regulating D2 receptor transcriptional activity was investigated in D2 receptor expressing neuroblastoma cells, NB41A3, and in non-D2 receptor expressing CHO cells. Deletion of a region containing the putative AP-1 binding site resulted in a significant reduction in the activity in CHO cells; while the activity in NB41A3 cells was increased suggesting that the AP-1 site may differentially regulate D2 gene expression in these distinct cell types. However, both cell lines were found to express significant and similar levels of the transcription factors AP-1. Analysis of phosphorylated proteins in each of the cell lines provided evidence that AP-1 is phosphorylated in NB41A3 cells, but not in CHO cells. This result suggests that differential regulation of D2 gene expression may be related to AP-1 phosphorylation.
Wang
J
J
Milahuser Laboratories, New York University School of Medicine, New York, USA. wangj@is2.nyu.edu
Miller
J C
JC
Friedhoff
A J
AJ
D000818:Animals; D001483:Base Sequence; D016466:CHO Cells / Q000502:physiology; D002478:Cells, Cultured; D002795:Choline O-Acetyltransferase / Q000235:genetics; D006224:Cricetinae; D004268:DNA-Binding Proteins / Q000378:metabolism; D017353:Gene Deletion; D005786:Gene Expression Regulation / Q000502:physiology; D017930:Genes, Reporter; D008099:Liver / Q000737:chemistry / Q000166:cytology / Q000201:enzymology; D008969:Molecular Sequence Data; D016296:Mutagenesis / Q000502:physiology; D010766:Phosphorylation; D011401:Promoter Regions, Genetic / Q000502:physiology; D051381:Rats; D017448:Receptors, Dopamine D2 / Q000235:genetics*; D015183:Restriction Mapping; D016329:Sp1 Transcription Factor / Q000378:metabolism; D018808:Transcription Factor AP-1 / Q000378:metabolism*; D050656:Transcription Factor AP-2; D014157:Transcription Factors / Q000378:metabolism
D004268:DNA-Binding Proteins; D017448:Receptors, Dopamine D2; D016329:Sp1 Transcription Factor; D018808:Transcription Factor AP-1; D050656:Transcription Factor AP-2; D014157:Transcription Factors; D002795:Choline O-Acetyltransferase