Identification of yebG as a DNA damage-inducible Escherichia coli gene.
The nucleotide sequence upstream of the Escherichia coli yebG gene presents features similar to those found in SOS system regulatory sites (putative SOS box, -10 and -35 promoter boxes and a ribosome binding site). Operon fusion assays demonstrate now that this region controls transcription in a recA-, lexA-dependent way and that the reporter gene expression is inducible by DNA damage consequent to mitomycin C treatment. Increased expression does not result from an increase in plasmid copy number. These results indicate that yebG is a novel SOS regulon gene. The yebG product is predicted to be a 96 amino acid residue, 10.7 kDa protein whose function is not yet known. Unlike other SOS genes, the construct carrying the yebG regulatory region is not stationary phase inducible.
Lomba
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MR
Instituto de Biofisica Carlos Chagas Filho, Universidade Federal do Rio de Janeiro, Brazil. darcy@lncc.br
Vasconcelos
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AT
Pacheco
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AB
de Almeida
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DF
D001483:Base Sequence; D016384:Consensus Sequence; D004249:DNA Damage / Q000235:genetics*; D004269:DNA, Bacterial / Q000235:genetics; D004926:Escherichia coli / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism; D005798:Genes, Bacterial; D008969:Molecular Sequence Data; D009876:Operon; D018085:Regulon; D013014:SOS Response, Genetics / Q000235:genetics
D004269:DNA, Bacterial