Evaluation of collagen gel and hyaluronic acid as vitreous substitutes.
We evaluated alkaline-solubilized collagen, hyaluronic acid (HA), and a substance formed from mixing both materials as vitreous substitutes in the rabbit. Fluorescein isothiocyanate (FITC)-labeled collagen (2%), fluorescein-labeled HA (FLHA, 1%), or the mixture was injected into the rabbit vitreous after vitrectomy. After 3 months, histologic study revealed that the substitutes caused no adverse effects on the ocular tissue. Kinetic studies showed that the half-lives of FITC-collagen, FL-HA, and the mixed substance were 5.70, 2.09, and 8.41 days, respectively. The results indicated that HA enhances the collagen half-life. The concomitant of collagen and HA is safe and effective for 3 months in rabbit's eye as a vitreous substitute, and the mixture is capable of tamponade.
Nakagawa
M
M
Department of Ophthalmology, Juntendo University, Tokyo, Japan.
Tanaka
M
M
Miyata
T
T
D000818:Animals; D003094:Collagen / Q000008:administration & dosage* / Q000009:adverse effects / Q000493:pharmacokinetics; D004341:Drug Evaluation; D004596:Electroretinography; D015590:Fluorophotometry; D005500:Follow-Up Studies; D005782:Gels; D006207:Half-Life; D006820:Hyaluronic Acid / Q000008:administration & dosage* / Q000009:adverse effects / Q000493:pharmacokinetics; D007267:Injections; D011817:Rabbits; D012160:Retina / Q000187:drug effects / Q000502:physiology; D012449:Safety; D014821:Vitrectomy; D014822:Vitreous Body / Q000502:physiology* / Q000648:ultrastructure
D005782:Gels; D006820:Hyaluronic Acid; D003094:Collagen