title: Survival of Toxoplasma gondii tissue cysts in 0.85-6% NaCl solutions at 4-20 C.
abstract: The effect of storage of Toxoplasma gondii tissue cysts in various NaCl solutions at different temperatures was studied. Tissue cysts from rodent brains were suspended in 0.85%, 2.0%, 3.3%, and 6.0% aqueous NaCl solutions. After storage at 4-20 C for various time intervals, brains were bioassayed in mice for viability of T. gondii. At 4 C, tissue cysts survived for at least 56 days in 0.85% NaCl, for 49 days in 2.0% NaCl, and for 21 days in 3.3% NaCl solutions. At 10 C, tissue cysts survived for at least 21 days in 0.85%, 2.0%, and 3.3% NaCl solutions. At 15 C, tissue cysts survived for at least 21 days in 0.85% NaCl, 14 days in 2.0%, and 3.3% NaCl solutions. At 20 C, tissue cysts survived for 14 days in 0.85% NaCl, 7 days in 2.0% NaCl, and 3 days in 3.3% NaCl solutions. Tissue cysts generally did not survive in 6.0% NaCl solution at any temperature.
authors.affiliation: U.S. Department of Agriculture, Livestock and Poultry Sciences Institute, Beltsville, Maryland 20705-2350, USA.
authors.forename: J P
authors.initials: JP
authors.lastname: Dubey
mesh_terms: D000818:Animals; D001681:Biological Assay; D001921:Brain / Q000469:parasitology; D005260:Female; D005519:Food Preservation; D008168:Lung / Q000469:parasitology; D051379:Mice; D051381:Rats; D017207:Rats, Sprague-Dawley; D012965:Sodium Chloride / Q000494:pharmacology*; D012996:Solutions; D013696:Temperature; D013997:Time Factors; D014122:Toxoplasma / Q000187:drug effects / Q000502:physiology*
chemical_list: D012996:Solutions; D012965:Sodium Chloride