title: A novel system of human submandibular/sublingual saliva collection.
abstract: The assessment of submandibular/sublingual (sm/sl) saliva is a procedure of increasing significance. However, the collection of these fluids by traditional techniques is difficult and therefore often neglected. To collect sm/sl saliva, we have assembled a novel, universal system consisting of four parts-collecting tubing, a buffering chamber, a storing tube, and a suction device. Submandibular/sublingual saliva samples were collected from ten healthy and ten xerostomic individuals. The system showed intra-examiner reproducibility of 0.92 for healthy and 0.89 for xerostomic subjects, and inter-examiner reproducibility of 0.93 for normal subjects and 0.80 for xerostomic individuals. The flow rates obtained by the present collecting set-up were similar to those measured by all known previous methods that were published during the last 40 years. The system was also efficient, in that more than 90% of the fluid that entered the system was eventually collected in the storing tube for analysis. The system appears to collect relatively pure sm/sl fluids, since contamination of the collected sample by a stimulant solution swabbed repeatedly over the tongue during saliva collection was minimal. The system is reliable, safe, practical, and comfortable for the patient.
authors.affiliation: Department of Oral Pathology and Oral Medicine, Maurice and Gabriela Goldschleger School of Dental Medicine, Tel Aviv University, Israel.
authors.forename: A
authors.initials: A
authors.lastname: Wolff
authors.forename: A
authors.initials: A
authors.lastname: Begleiter
authors.forename: D
authors.initials: D
authors.lastname: Moskona
mesh_terms: D000328:Adult; D019343:Citric Acid / Q000008:administration & dosage; D004867:Equipment Design; D005260:Female; D005840:Gentian Violet / Q000008:administration & dosage; D006801:Humans; D008297:Male; D015588:Observer Variation; D012463:Saliva / Q000737:chemistry* / Q000187:drug effects; D012636:Secretory Rate / Q000187:drug effects; D012680:Sensitivity and Specificity; D013048:Specimen Handling / Q000295:instrumentation* / Q000379:methods / Q000706:statistics & numerical data; D013053:Spectrophotometry / Q000706:statistics & numerical data; D013268:Stimulation, Chemical; D013361:Sublingual Gland / Q000378:metabolism*; D013363:Submandibular Gland / Q000378:metabolism*; D013997:Time Factors; D014987:Xerostomia / Q000503:physiopathology
chemical_list: D019343:Citric Acid; D005840:Gentian Violet