title: Peroxidase activity of a TSA-like antioxidant protein from a pathogenic amoeba.
abstract: The 29 kDa surface protein of Entamoeba histolytica is an abundant antigenic protein expressed by pathogenic strains of this organism. The protein is a member of a widely-dispersed group of homologues which includes at least two cysteinyl peroxidases, Salmonella typhimurium alkyl hydroperoxidase C-22 protein (AhpC) and Saccharomyces cerevisiae thiol-specific antioxidant protein (TSA). Here, for the first time in a pathogenic eukaryote, we have demonstrated that the amoebic protein also possesses peroxidatic and antioxidant activities in the presence of reductants such as dithiothreitol or thioredoxin reductase plus thioredoxin. Although the S. typhimurium AhpF flavoprotein was not an effective reductant of the amoebic TSA protein, one inhibitory monoclonal antibody directed toward amoebic TSA was also partially inhibitory toward reduced but not oxidized bacterial AhpC. These antioxidant proteins are likely to be important not only in general cell protection, but also in the promotion of infection and invasion by these pathogenic organisms through protection against oxidative attack by activated host phagocytic cells.
authors.affiliation: Department of Biochemistry, Wake Forest University Medical Center, Winston-Salem, NC, 27157-1016, USA. lbpoole@bgsm.edu
authors.forename: L B
authors.initials: LB
authors.lastname: Poole
authors.forename: H Z
authors.initials: HZ
authors.lastname: Chae
authors.forename: B M
authors.initials: BM
authors.lastname: Flores
authors.forename: S L
authors.initials: SL
authors.lastname: Reed
authors.forename: S G
authors.initials: SG
authors.lastname: Rhee
authors.forename: B E
authors.initials: BE
authors.lastname: Torian
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000494:pharmacology; D000975:Antioxidants / Q000378:metabolism*; D003429:Cross Reactions; D004748:Entamoeba histolytica / Q000201:enzymology* / Q000276:immunology / Q000472:pathogenicity; D005974:Glutamate-Ammonia Ligase / Q000187:drug effects / Q000378:metabolism; D010084:Oxidation-Reduction / Q000187:drug effects; D010088:Oxidoreductases / Q000276:immunology; D009195:Peroxidase / Q000378:metabolism*; D010544:Peroxidases; D054464:Peroxiredoxins; D011506:Proteins / Q000276:immunology / Q000378:metabolism*; D015800:Protozoan Proteins / Q000276:immunology / Q000378:metabolism*; D012486:Salmonella typhimurium / Q000201:enzymology / Q000276:immunology
chemical_list: D000911:Antibodies, Monoclonal; D000975:Antioxidants; D011506:Proteins; D015800:Protozoan Proteins; D010088:Oxidoreductases; D010544:Peroxidases; D054464:Peroxiredoxins; D009195:Peroxidase; D005974:Glutamate-Ammonia Ligase