[Mutations of p53 gene exons 5 to 8 in human ovarian cancer and its clinical significance].
OBJECTIVE
To detect p53 mutations in human ovarian cancer and to look for correlations between p53 mutation and clinicopathologic features.


METHODS
Mutations in exons 5-8 of p53 gene in 41 patients with primary ovarian cancer were screened by polymerase chain reaction-single strand conformation polymorphism analysis with non-isotopic silver staining method.


RESULTS
Mutations of p53 were found in 17 cases (41.5%); p53 mutation was not correlated with the age, the FIGO stage and the histologic type of the patients. However, it was associated with grade and lymph node metastasis in a univariate analysis.


CONCLUSIONS
Mutations of p53 occur commonly in human ovarian cancer. p53 mutation is an early event in ovarian carcinogenesis and may serve as a genetic marker for early diagnosis and prognosis.
You
Z
Z
First Affiliated Hospital, Beijing Medical University.
Guo
Y
Y
Wen
H
H
D000293:Adolescent; D000328:Adult; D000368:Aged; D005091:Exons; D005260:Female; D016158:Genes, p53 / Q000235:genetics*; D006801:Humans; D008207:Lymphatic Metastasis; D008875:Middle Aged; D009154:Mutation; D010051:Ovarian Neoplasms / Q000235:genetics* / Q000473:pathology; D016133:Polymerase Chain Reaction; D018807:Polymorphism, Single-Stranded Conformational