alpha 1-Acid glycoprotein reduces local and remote injuries after intestinal ischemia in the rat.
The aim of this study was to look at the role of alpha 1-acid glycoprotein as a natural anti-inflammatory agent with particular respect to its antineutrophil and anticomplement activity. A recombinantly engineered form of sialyl Lewisx (sLe(x))-bearing alpha 1-acid glycoprotein (sAGP) was administered intravenously to pentobarbital-anesthetized rats after 50 min of intestinal ischemia just before 4 h of reperfusion. A non-sLe(x)-bearing form of AGP (nsAGP) was used as control. sAGP-treated animals had a 62% reduction (P < 0.05) in remote lung injury, assessed by 125I-albumin permeability, compared with those treated with nsAGP (permeability index of 3.61 +/- 0.15 x 10(-3) and 5.18 +/- 0.67 x 10(-3), respectively). There was a reduction in pulmonary myeloperoxidase levels in sAGP-treated rats compared with nsAGP-treated rats. Complement-dependent intestinal injury, assessed by 125I-albumin permeability was reduced by 28% (P < 0.05) in animals treated with sAGP (7.58 +/- 0.63) compared with those treated with nsAGP (10.4 +/- 0.54). We conclude that sAGP ameliorates both complement- and neutrophil-mediated injuries.
Williams
J P
JP
Department of Surgery, Brigham and Women's Hospital, Boston, Massachusetts 02115, USA.
Weiser
M R
MR
Pechet
T T
TT
Kobzik
L
L
Moore
F D
FD
Hechtman
H B
HB
D000818:Animals; D002463:Cell Membrane Permeability; D003170:Complement Pathway, Alternative / Q000187:drug effects; D006801:Humans; D007422:Intestines / Q000098:blood supply* / Q000187:drug effects / Q000473:pathology; D007457:Iodine Radioisotopes; D007511:Ischemia / Q000503:physiopathology* / Q000517:prevention & control; D008168:Lung / Q000187:drug effects / Q000473:pathology*; D008297:Male; D008641:Mesenteric Vascular Occlusion; D009844:Oligosaccharides; D009961:Orosomucoid / Q000494:pharmacology*; D009195:Peroxidase / Q000032:analysis; D051381:Rats; D017207:Rats, Sprague-Dawley; D011994:Recombinant Proteins / Q000494:pharmacology; D015424:Reperfusion; D000080506:Sialyl Lewis X Antigen
D007457:Iodine Radioisotopes; D009844:Oligosaccharides; D009961:Orosomucoid; D011994:Recombinant Proteins; D000080506:Sialyl Lewis X Antigen; D009195:Peroxidase