Transcription of the mouse mu-opioid receptor gene is regulated by two promoters.
Two transcription initiation sites have been identified in the mouse mu-opioid receptor (MOR) gene at approximately -793 and -268 upstream of the translation start site. To test if the MOR gene contains two functional promoters, chimeric constructs of mouse MOR deletion fragments, fused to a luciferase reporter gene, were transiently transfected into the neuroblastoma cell line SK-N-SH, a MOR-expressing cell line, and two MOR-non-expressing cell lines. Results from transient transfection assays confirmed the existence of two functional independent promoters in the mouse MOR gene, and also revealed that the region from -1337 to -93 does not contain all the elements necessary to confer tissue-specific expression of the MOR gene.
Liang
Y
Y
Department of Pharmacology and Toxicology, Indianapolis, IN 46202-5121, USA.
Carr
L G
LG
D000818:Animals; D002678:Chimera / Q000235:genetics; D017353:Gene Deletion; D017930:Genes, Reporter / Q000502:physiology; D008156:Luciferases / Q000235:genetics; D051379:Mice; D011401:Promoter Regions, Genetic / Q000502:physiology*; D017450:Receptors, Opioid, mu / Q000235:genetics* / Q000378:metabolism; D014158:Transcription, Genetic / Q000502:physiology*; D014162:Transfection; D014407:Tumor Cells, Cultured / Q000378:metabolism
D017450:Receptors, Opioid, mu; D008156:Luciferases