title: New monoclonal antibodies to oestrogen and progesterone receptors effective for paraffin section immunohistochemistry.
abstract: Assessment of oestrogen and progesterone receptors (ER and PgR) in breast cancer is widely used for the prediction of response to endocrine therapy and as a prognostic marker. Cytosolic assays have been replaced in many centres by immunochemical techniques, which have many advantages including applicability to small samples, simplicity, and cost-effectiveness. This study describes the generation and characterisation of two novel murine monoclonal antibodies recognizing ER and PgR, designated NCL-ER-6F11 and NCL-PGR respectively, which are effective in heat-treated formalin-fixed, paraffin-embedded tissue. The antibodies have been characterized by Western blotting and by immunohistochemistry on normal and pathological breast and other tissues. NCL-ER-6F11 has been shown to compare favourably with a currently available ER antibody. These antibodies may prove of value in the assessment of hormone receptor status in human breast cancer.
authors.affiliation: Novocastra Laboratories Ltd., Newcastle upon Tyne, U.K.
authors.forename: D J
authors.initials: DJ
authors.lastname: Bevitt
authors.forename: I D
authors.initials: ID
authors.lastname: Milton
authors.forename: N
authors.initials: N
authors.lastname: Piggot
authors.forename: L
authors.initials: L
authors.lastname: Henry
authors.forename: M J
authors.initials: MJ
authors.lastname: Carter
authors.forename: G L
authors.initials: GL
authors.lastname: Toms
authors.forename: T W
authors.initials: TW
authors.lastname: Lennard
authors.forename: B
authors.initials: B
authors.lastname: Westley
authors.forename: B
authors.initials: B
authors.lastname: Angus
authors.forename: C H
authors.initials: CH
authors.lastname: Horne
mesh_terms: D000911:Antibodies, Monoclonal / Q000096:biosynthesis*; D014408:Biomarkers, Tumor / Q000276:immunology* / Q000378:metabolism; D015153:Blotting, Western; D001943:Breast Neoplasms / Q000378:metabolism*; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D016612:Paraffin Embedding; D011960:Receptors, Estrogen / Q000276:immunology* / Q000378:metabolism; D011980:Receptors, Progesterone / Q000276:immunology* / Q000378:metabolism; D014407:Tumor Cells, Cultured
chemical_list: D000911:Antibodies, Monoclonal; D014408:Biomarkers, Tumor; D011960:Receptors, Estrogen; D011980:Receptors, Progesterone