Site-specific mutagenesis of immunoglobulin domains by multiple-fragment homologous recombination.
Eghtedarzadeh-Kondri
M
M
Department of Molecular Immunology, Bristol-Myers Squibb Pharmaceutical Research Institute, Seattle, WA 98121, USA.
Walls
M A
MA
Glaser
S M
SM
D000818:Animals; D017104:Bacteriophage M13 / Q000235:genetics; D019556:COS Cells; D015252:Deoxyribonucleases, Type II Site-Specific / Q000378:metabolism; D004926:Escherichia coli / Q000235:genetics; D005803:Genes, Immunoglobulin / Q000235:genetics*; D005822:Genetic Vectors; D006801:Humans; D007140:Immunoglobulin Fab Fragments / Q000235:genetics; D007074:Immunoglobulin G / Q000235:genetics*; D016297:Mutagenesis, Site-Directed; D016133:Polymerase Chain Reaction; D011994:Recombinant Proteins; D015183:Restriction Mapping; D014162:Transfection
D007140:Immunoglobulin Fab Fragments; D007074:Immunoglobulin G; D011994:Recombinant Proteins; C039257:AGCGCT-specific type II deoxyribonucleases; D015252:Deoxyribonucleases, Type II Site-Specific