Characterization of the oligosaccharide component of arylsulfatase B from rat liver.
A glycan chain analysis of the total oligosaccharide pool derived from rat liver arylsulfatase B was carried out by. P4 Gel Permeation Chromatography and sequential exoglycosidase digestion. It was found that 71% of rat liver arylsulfatase B oligosaccharides were sialylated. The relative contribution of particular structures in the total glycan pool was as follows: sialylated biantennary complex type glycans with terminal galactose--65%, high-mannose type glycans--15%, biantennary complex type glycans with core fucose and terminal N-acetylglucosamine--5%, O-linked oligosaccharides--3.5%.
Przybyło
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Department of Animal Physiology, Jagiellonian University, Cracow, Poland. kloc@zuk.iz.uj.edu.pl
Lityńska
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D000818:Animals; D002803:Chondro-4-Sulfatase / Q000032:analysis* / Q000302:isolation & purification; D002854:Chromatography, Paper / Q000379:methods; D004591:Electrophoresis, Polyacrylamide Gel; D008099:Liver / Q000201:enzymology*; D008297:Male; D009844:Oligosaccharides / Q000032:analysis* / Q000302:isolation & purification; D011134:Polysaccharides / Q000032:analysis / Q000302:isolation & purification; D051381:Rats; D017208:Rats, Wistar
D009844:Oligosaccharides; D011134:Polysaccharides; D002803:Chondro-4-Sulfatase