Translational control of terminal oligopyrimidine mRNAs requires a specific regulator.
Terminal oligopyrimidine (TOP) mRNAs are a group of messengers translationally regulated according to the growth status of the cell. Two hypotheses have been proposed for the mechanism of the regulation: (i) there is a specific translational regulator which can reversibly alter TOP-mRNA structure, (ii) a component of the general translational apparatus can specifically affect the translation of TOP-mRNAs. To verify one of the two hypotheses we induced a partial inhibition of translation initiation in Xenopus cultured cells and analyzed the effect on TOP-mRNA translation. Our results suggest that a specific regulator is necessary to explain the translational control of these of mRNAs.
Loreni
F
F
Dipartimento di Biologia, Universitá Tor Vergata, Roma, Italy.
Amaldi
F
F
D000818:Animals; D002460:Cell Line; D016895:Culture Media, Serum-Free; D005786:Gene Expression Regulation; D006358:Hot Temperature; D007668:Kidney; D007700:Kinetics; D008957:Models, Genetic; D010142:Pactamycin / Q000494:pharmacology; D011132:Polyribosomes / Q000187:drug effects / Q000378:metabolism*; D014176:Protein Biosynthesis; D011500:Protein Synthesis Inhibitors / Q000494:pharmacology; D012333:RNA, Messenger / Q000737:chemistry* / Q000378:metabolism*; D014982:Xenopus laevis
D016895:Culture Media, Serum-Free; D011500:Protein Synthesis Inhibitors; D012333:RNA, Messenger; D010142:Pactamycin