Characterization of pathogenic fungi genomes using pulsed field gel electrophoresis.
Pulsed field gel electrophoresis (PFGE) has been firstly introduced in characterization of the pathogenic fungi Penicillium marneffei and Exophiala dermatitidis genomes. The numbers and sizes of their chromosomes have been detected. Polymorphism was identified on the smallest chromosome of E. dermatitidis. The result shows that PFGE for characterization of large molecular DNA pathogenic fungi is very suitable, it is more simple and more efficacy. The result also shows the diversity of pathogenic fungi is relative common even in rare occurred pathogenic fungi such as E. dermatitidis.
Wu
S
S
Institute of Dermatology, CAMS, Nanjing.
Guo
N
N
Yin
Z
Z
Chai
J
J
D004271:DNA, Fungal; D016521:Electrophoresis, Gel, Pulsed-Field; D005093:Exophiala / Q000235:genetics*; D016681:Genome, Fungal; D010407:Penicillium / Q000235:genetics*
D004271:DNA, Fungal