title: Transcriptional regulation of SUP35 and SUP45 in Saccharomyces cerevisiae.
abstract: SUP35 and SUP45 encode translational release factors in the yeast Saccharomyces cerevisiae. In addition, Sup35p is related to the cytoplasmically inherited prion-like phenotype [PSI+]. The vital cellular role of Sup35p and Sup45p prompted us to study the regulation of transcription of the corresponding genes. Since the [PSI] state of the yeast strain affects the abundance of Sup35p and Sup45p, both [PSI+] and [psi-] variants were included in these analyses. It turned out that SUP35 and SUP45 transcript levels are regulated by nutritional changes and stress in a way strikingly similar to those of ribosomal protein genes. The [PSI] state did not influence the respective transcript levels nor their regulation, although HSP12 (as a monitor of general stress-responsive) gene expression appeared to differ in the two variant strains. The transcription activation sites of SUP35 and SUP45 were mapped using deletion analysis of the respective promoter-reporter fusion genes. The UAS in both cases was found to consist of an Abf1p-site and a T-rich element. Also in this respect SUP35 and SUP45 show a notable resemblance with ribosomal protein genes. Evidence was found that SUP35 in addition harbors a potential internal promoter element which became active after progressive 5'-deletion removing the first of the three in-frame ATGs.
authors.affiliation: Department of Biochemistry and Molecular Biology, IMBW, BioCentrum Amsterdam, Vrije Universiteit, The Netherlands.
authors.forename: A
authors.initials: A
authors.lastname: Dagkessamanskaya
authors.forename: M
authors.initials: M
authors.lastname: Ter-Avanesyan
authors.forename: W H
authors.initials: WH
authors.lastname: Mager
mesh_terms: D001483:Base Sequence; D004252:DNA Mutational Analysis; D005656:Fungal Proteins / Q000096:biosynthesis / Q000235:genetics*; D015966:Gene Expression Regulation, Fungal; D017930:Genes, Reporter; D008969:Molecular Sequence Data; D016296:Mutagenesis; D010454:Peptide Termination Factors; D011328:Prions; D011401:Promoter Regions, Genetic; D012331:RNA, Fungal / Q000032:analysis; D012333:RNA, Messenger / Q000032:analysis; D012441:Saccharomyces cerevisiae / Q000235:genetics*; D029701:Saccharomyces cerevisiae Proteins; D017384:Sequence Deletion; D013489:Suppression, Genetic; D014158:Transcription, Genetic
chemical_list: D005656:Fungal Proteins; D010454:Peptide Termination Factors; D011328:Prions; D012331:RNA, Fungal; D012333:RNA, Messenger; C068068:SUP35 protein, S cerevisiae; C080775:SUP45 protein, S cerevisiae; D029701:Saccharomyces cerevisiae Proteins