title: Diffusion and transfer of antibody proteins from a sugar-based hydrogel.
abstract: Diffusion of antibody protein from hydrogel films and hydrogel encapsulated in a microcapillary was studied. Thin hydrogel films were formed by crosslinking 6-acryloyl-B-O-methylgalactoside with N,N'-methylene-bis-acrylamide and the diffusive transport of monoclonal antimouse IgG-FITC into and out of the hydrate films was measured. Diffusion coefficients in 2 and 4% crosslinked hydrogel films were measured. The measured diffusion constants determined for IgG in both the 2 and 4% hydrogel films were comparable to the free diffusion of IgG in bulk water (Dmean approximately 10(-7) cm2/s). In addition, 2% crosslinked hydrogels were prepared in a capillary tube and the transport of antimouse IgG-FITC into and out of the hydrated hydrogel was measured. Kinetic analysis indicated that the protein transport through the capillary hydrogel was faster than would be expected for a simple diffusion process. Finally, by utilizing the diffusion of antibody from the capillary hydrogel, transfer of antibody to a silica surface was demonstrated. A capillary hydrogel loaded with antimouse IgG-FITC was used to transfer the protein to a silica surface forming a 30-micron spot of antibody, which was imaged using fluorescence microscopy. These results may lead to the development of a nonlithographic method of patterning antibodies on surfaces for use in integrated microimmunosensors.
authors.affiliation: Laboratory for Molecular Interfacial Interactions, Naval Research Laboratory, Washington, D.C. 20375, USA.
authors.forename: M A
authors.initials: MA
authors.lastname: Markowitz
authors.forename: D C
authors.initials: DC
authors.lastname: Turner
authors.forename: B D
authors.initials: BD
authors.lastname: Martin
authors.forename: B P
authors.initials: BP
authors.lastname: Gaber
mesh_terms: D000178:Acrylamides; D000818:Animals; D000911:Antibodies, Monoclonal / Q000378:metabolism*; D002197:Capillary Action; D003432:Cross-Linking Reagents; D004058:Diffusion; D016650:Fluorescein-5-isothiocyanate; D005456:Fluorescent Dyes; D006863:Hydrogen-Ion Concentration; D007074:Immunoglobulin G / Q000378:metabolism; D008756:Methylgalactosides; D051379:Mice; D008856:Microscopy, Fluorescence; D011108:Polymers
chemical_list: D000178:Acrylamides; D000911:Antibodies, Monoclonal; D003432:Cross-Linking Reagents; D005456:Fluorescent Dyes; D007074:Immunoglobulin G; D008756:Methylgalactosides; D011108:Polymers; C109801:hydrogel film; C021221:N,N'-methylenebisacrylamide; D016650:Fluorescein-5-isothiocyanate