Increased sensitivity of scid heterozygous mice to ionizing radiation.
In the present study, acute effects of ionizing radiation on animal survival, bone marrow cells and fibroblast cell lines of scid homozygous, scid heterozygous and wild-type mice with the same C.B-17 genetic background were examined. The sensitivities to ultraviolet light (UV) and various chemicals, bleomycin, mitomycin C, N-methyl-N'-nitro-N-nitrosoguanidine, methyl methanosulphonate, 5-fluorouracil, 6-mercaptopurine, 4-nitroquinoline 1-oxide and potassium bromate) were also investigated. In addition, micronucleus testing of whole-body irradiated mice was performed. Scid heterozygous mice were found to be less sensitive than the homozygotes but more sensitive to ionizing radiation than wild-type mice, not only in vivo but also for bone marrow cells in vitro, suggesting partial dominance under both conditions. In contrast, there were no differences in sensitivity to UV light and various chemicals, as compared with wild-type and scid heterozygous cell lines, either in vitro or in the micronucleus test.
Kobayashi
S
S
Low Dose Radiation Risk and Carcinogenesis Research Group, National Institute of Radiological Sciences, Chiba, Japan.
Nishimura
M
M
Shimada
Y
Y
Suzuki
F
F
Matsuoka
A
A
Sakamoto
H
H
Hayashi
M
M
Sofuni
T
T
Sado
T
T
Ogiu
T
T
D000818:Animals; D001854:Bone Marrow Cells / Q000528:radiation effects; D002460:Cell Line; D006579:Heterozygote; D051379:Mice; D008813:Mice, Inbred ICR; D016513:Mice, SCID / Q000235:genetics*; D048629:Micronuclei, Chromosome-Defective / Q000528:radiation effects; D011836:Radiation Tolerance