title: Alterations of AMPA-selected glutamate subtype immunoreactivity in the dentate gyrus after perforant pathway lesion.
abstract: Immunocytochemical techniques were employed to examine the changes in immunolabeling of the alpha-amino-3-hydroxy-5-methyl-4-isoaxolepropionate (AMPA) receptor subunits GluR1 and GluR2/3 within the dentate gyrus 1, 3, 7, 14, 30, and 90 days after a unilateral perforant pathway lesion in the rat brain. Completeness of the lesion was confirmed following examination of Nissl-stained tissue sections at all times post-lesion and acetylcholinesterase (AChE)-stained sections 14, 30 and 90 days post-lesion, the latter providing evidence of compensatory sprouting of cholinergic fibers in the outer molecular layer of the dentate gyrus. Compared to the non-lesioned hippocampus there was no difference in the staining pattern of AMPA receptor subunits in the dentate gyrus of the deafferented hippocampus 1, 3, 7 and 14 days following lesioning of the perforant pathway. In contrast, 30 and 90 days post-lesion, GluR1 immunolabeling was increased in the outer molecular layer of the dentate gyrus (i.e., deafferented zone) ipsilateral to lesion. Likewise, GluR2/3 immunolabeling was increased within the same region although the intensity of the response was less than that which was observed for GluR1. These data suggest that the loss of the perforant pathway fibers results in a compensatory increase in GluR1 and to a lesser extent GluR2/3 immunolabeling of the outer molecular layer at 30 and 90 days post-lesion and further suggest that AMPA receptor subunits play a role in perforant pathway signal transduction.
authors.affiliation: Neuroscience Research Center, MCP Hahnemann School of Medicine, Allegheny-Campus, Pittsburgh, PA 15212, USA.
authors.forename: K
authors.initials: K
authors.lastname: Mizukami
authors.forename: A
authors.initials: A
authors.lastname: Mishizen
authors.forename: M D
authors.initials: MD
authors.lastname: Ikonomovic
authors.forename: R
authors.initials: R
authors.lastname: Sheffield
authors.forename: D M
authors.initials: DM
authors.lastname: Armstrong
authors.forename: A
authors.initials: A
authors.lastname: Mishizin
mesh_terms: D000818:Animals; D018891:Dentate Gyrus / Q000737:chemistry*; D018698:Glutamic Acid / Q000032:analysis*; D007150:Immunohistochemistry; D008297:Male; D019580:Perforant Pathway / Q000502:physiology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D018350:alpha-Amino-3-hydroxy-5-methyl-4-isoxazolepropionic Acid / Q000032:analysis*
chemical_list: D018698:Glutamic Acid; D018350:alpha-Amino-3-hydroxy-5-methyl-4-isoxazolepropionic Acid