title: Complete nucleotide sequence of Colorado tick fever virus segments M6, S1 and S2.
abstract: The nucleotide sequences of the tenth (M6), eleventh (S1) and twelfth (S2) dsRNA genomic segments of the Florio strain (N-7180) of Colorado tick fever virus were determined and found to be 675, 998 and 1884 bp, respectively, in length. A nonanucleotide motif and a hexanucleotide motif were found to be highly conserved in the 5' and 3' non-coding regions (NCRs), respectively, of the three segments. The first and last three nucleotides of each segment were of inverted complementarity, and segment-specific inverted terminal repeats were detected in the NCRs of the three segments. These findings suggest the occurrence of intracellular panhandle structures for the RNA transcripts. A readthrough phenomenon is suspected in segment M6. The environment surrounding the opal codon (position 1052-1054) of segment M6 conforms to that of leaky opal codons described in the literature.
authors.affiliation: Laboratoire de Virologie Moléculaire, Tropicale et Transfusionnelle, Faculté de Médecine de Marseille, Université de la Méditerranée, France.
authors.forename: H
authors.initials: H
authors.lastname: Attoui
authors.forename: P
authors.initials: P
authors.lastname: De Micco
authors.forename: X
authors.initials: X
authors.lastname: de Lamballerie
mesh_terms: D000818:Animals; D001483:Base Sequence; D003122:Colorado tick fever virus / Q000235:genetics*; D016679:Genome, Viral; D008969:Molecular Sequence Data; D012367:RNA, Viral / Q000235:genetics*; D012091:Repetitive Sequences, Nucleic Acid; D017421:Sequence Analysis
chemical_list: D012367:RNA, Viral