title: The cDNA cloning and molecular evolution of reptile and pigeon lactate dehydrogenase isozymes.
abstract: The cDNAs encoding lactate dehydrogenase isozymes LDH-A (muscle) and LDH-B (heart) from alligator and turtle and LDH-A, LDH-B, and LDH-C (testis) from pigeon were cloned and sequenced. The evolutionary relationships among vertebrate LDH isozymes were analyzed. Contrary to the traditional belief that the turtle lineage branched off before the divergence between the lizard/alligator and bird lineages, the turtle lineage was found to be clustered with either the alligator lineage or the alligator-bird clade, while the lizard lineage was found to have branched off before the divergence between the alligator/turtle and bird lineages. The pigeon testicular LDH-C isozyme was evidently duplicated from LDH-B (heart), so it is not orthologous to the mammalian testicular LDH-C isozymes.
authors.affiliation: Laboratory of Molecular Genetics, Institute of Environmental Health Sciences, National Institutes of Health, Research Triangle Park, North Carolina, USA.
authors.forename: H
authors.initials: H
authors.lastname: Mannen
authors.forename: S C
authors.initials: SC
authors.lastname: Tsoi
authors.forename: J S
authors.initials: JS
authors.lastname: Krushkal
authors.forename: W H
authors.initials: WH
authors.lastname: Li
authors.forename: S S
authors.initials: SS
authors.lastname: Li
mesh_terms: D000489:Alligators and Crocodiles / Q000235:genetics; D000595:Amino Acid Sequence; D000818:Animals; D003001:Cloning, Molecular; D010856:Columbidae / Q000235:genetics*; D018076:DNA, Complementary / Q000235:genetics*; D019143:Evolution, Molecular; D005260:Female; D007527:Isoenzymes; D007770:L-Lactate Dehydrogenase / Q000235:genetics*; D008116:Lizards / Q000235:genetics; D008297:Male; D008969:Molecular Sequence Data; D009132:Muscles / Q000201:enzymology; D009206:Myocardium / Q000201:enzymology; D010802:Phylogeny; D012104:Reptiles / Q000235:genetics*; D017386:Sequence Homology, Amino Acid; D009626:Terminology as Topic; D013737:Testis / Q000201:enzymology
chemical_list: D018076:DNA, Complementary; D007527:Isoenzymes; D007770:L-Lactate Dehydrogenase