title: Autologous connective tissue bars as vehicles for the administration of growth-influencing substances to the brain.
abstract: One of the main problems in the introduction of growth factors or other substances into the CNS is that most of these do not pass the blood-brain barrier, and so they have to be delivered directly to the target cells. Recently, different methods of intracerebral administration have been introduced such as release of drugs from polymer matrix or hollow polymer fibres, implantation of solution-soaked gel foam pieces or genetically modified cells secreting growth factors. In our studies on regeneration in CNS, the problem of introducing active substances into the brain arose. For this reason we elaborated a new method for the administration of soluble factor by means of autologous connective tissue chambers filled with fibrin. This method is particularly suitable in studies in which the visualization of the regrowth of nerve fibres is one of the main purposes. Presence of an active substance inside the chamber fibres to grow in the direction of the chamber and such fibres can be visualized here. Such visualization is impossible while using other methods of trophic substance delivery into the CNS, e.g. intraventricular steel cannula connected to an osmotic minipump, because there is neither space nor appropriate milieu to allow the fibres' ingrowth. Retrograde tracing methods allow to establish the cells of origin of these fibres. This method is inexpensive, simple and adaptable to histological procedures.
authors.affiliation: Department of Physiology, Silesian Medical School, Katowice, Poland.
authors.forename: J J
authors.initials: JJ
authors.lastname: Barski
authors.forename: J
authors.initials: J
authors.lastname: Lewin-Kowalik
authors.forename: M
authors.initials: M
authors.lastname: Krause
authors.forename: D
authors.initials: D
authors.lastname: Górka
authors.forename: M
authors.initials: M
authors.lastname: Larysz-Brysz
authors.forename: B
authors.initials: B
authors.lastname: Gołka
authors.forename: E
authors.initials: E
authors.lastname: Swiech-Sabuda
mesh_terms: D000818:Animals; D001921:Brain / Q000187:drug effects*; D003238:Connective Tissue; D015194:Diffusion Chambers, Culture; D005337:Fibrin / Q000008:administration & dosage; D006133:Growth Substances / Q000008:administration & dosage* / Q000494:pharmacology; D008297:Male; D009412:Nerve Fibers / Q000502:physiology; D009416:Nerve Regeneration; D014677:Pharmaceutical Vehicles; D051381:Rats; D017208:Rats, Wistar
chemical_list: D006133:Growth Substances; D014677:Pharmaceutical Vehicles; D005337:Fibrin