title: A beta-glucosidase gene downstream of the cellulose synthase operon in cellulose-producing Acetobacter.
abstract: An open reading frame was found 214 bp downstream of the cellulose synthase operon of Acetobacter. The encoded amino acid sequence was found to be similar to some beta-glucosidases (G3ases). We detected G3ase activity in the culture medium and analysis of the N-terminal amino acid sequence showed that this gene encodes the enzyme. Therefore, it is possible that this region is a gene cluster for cellulose synthesis.
authors.affiliation: Bio-Polymer Research Co., Ltd., Kawasaki, Japan.
authors.forename: N
authors.initials: N
authors.lastname: Tonouchi
authors.forename: N
authors.initials: N
authors.lastname: Tahara
authors.forename: Y
authors.initials: Y
authors.lastname: Kojima
authors.forename: T
authors.initials: T
authors.lastname: Nakai
authors.forename: F
authors.initials: F
authors.lastname: Sakai
authors.forename: T
authors.initials: T
authors.lastname: Hayashi
authors.forename: T
authors.initials: T
authors.lastname: Tsuchida
authors.forename: F
authors.initials: F
authors.lastname: Yoshinaga
mesh_terms: D000091:Acetobacter / Q000201:enzymology / Q000235:genetics*; D000595:Amino Acid Sequence; D001483:Base Sequence; D002482:Cellulose / Q000096:biosynthesis*; D003470:Culture Media; D015971:Gene Expression Regulation, Enzymologic; D005798:Genes, Bacterial / Q000235:genetics*; D008969:Molecular Sequence Data; D005810:Multigene Family; D016366:Open Reading Frames; D009876:Operon / Q000235:genetics*; D001617:beta-Glucosidase / Q000235:genetics*
chemical_list: D003470:Culture Media; D002482:Cellulose; D001617:beta-Glucosidase