[Glucocorticoid-induced apoptosis of human gastric epithelial cells transfected with p53 genes].
To investigate the apoptosis-inducing effect of glucocorticoid on gastric epithelial cell expressing different p53 genes, a human gastric epithelial cell line-GES-1 was transfected with either wild type or mutant p53 cDNA in vitro. The cells were treated with hydrocortisone in a concentration range of 0.2-0.8 g/L. Apoptotic cells were found in those transfectants. The apoptotic response of the wild-type p53-transfected GES-1 cells was much more marked than that of the mutant p53 transfected ones. It can be inferred that the glucocorticoid secreted not only suppress the immunological response during inflammation, but induce gastric epithelial cell apoptosis as well. If the gastric epithelial cell contains mutant p53 genes, the glucocorticoid confer a selective growth advantage which advance the malignant process.
Zhang
J
J
Department of Genetics, Beijing Institute for Cancer Research, School of Oncology, Beijing Medical University.
Ke
Y
Y
Ning
T
T
D017209:Apoptosis / Q000187:drug effects*; D002460:Cell Line; D004847:Epithelial Cells; D005753:Gastric Mucosa / Q000166:cytology*; D016158:Genes, p53 / Q000235:genetics*; D006801:Humans; D006854:Hydrocortisone / Q000494:pharmacology*; D009154:Mutation; D014162:Transfection
D006854:Hydrocortisone