Mutagenicity from neutrophils after challenge with Helicobacter pylori and bile.
OBJECTIVE
To study some mechanisms involved in Helicobacter pylori (H. pylori)-induced gastric carcinogenesis.


METHODS
In vitro study.


METHODS
Medical centre hospital, Sweden.


METHODS
Mutagenicity in Ames' test of neutrophils challenged for 2 hours or more by two different strains of H. pylori. One strain designated NCTC 11637 by the National College of Type Cultures activated neutrophils to an oxidative burst and producing vacuolating cytotoxin, the other strain C-7050 lacked these abilities. Mutagenicity was also studied with sterile human gall bladder bile alone added to neutrophils or in combination with both neutrophils and H. pylori.


RESULTS
There was no increase in the number of revertants with the crude suspension or the supernatant of neutrophils challenged for 1 hour or less with H. pylori, bile, or the combination of both. However, in 5 out of 19 experiments there was significant mutagenicity after challenge of neutrophils for 2 hours or more with either strain of H. pylori, bile, or the combination of the two. The strongest mutagenicity was obtained after challenge over night (18 hours) with the combination of H. pylori and bile.


CONCLUSIONS
Mutagenicity occurs when neutrophils are challenged with H. pylori and bile. Factors other than reactive oxygen metabolites seem to be responsible.
Hagman
M
M
Department of Clinical Microbiology & Immunology, Orebro Medical Centre Hospital, Sweden.
Loogna
P
P
Danielsson
D
D
Domellöf
L
L
D001646:Bile; D001707:Biopsy, Needle; D002478:Cells, Cultured; D005753:Gastric Mucosa / Q000473:pathology; D016480:Helicobacter pylori / Q000472:pathogenicity*; D006801:Humans; D009152:Mutagenicity Tests; D009153:Mutagens; D009504:Neutrophils / Q000502:physiology*; D015203:Reproducibility of Results; D016897:Respiratory Burst; D012680:Sensitivity and Specificity
D009153:Mutagens