PDGF-stimulated calcium influx changes during in vitro cell transformation.
Platelet Derived Growth Factor (PDGF)-stimulated intracellular calcium signals were obtained from preneoplastic clones derived from C3H 10T1/2 mouse fibroblasts during a four-week transformation assay. By the end of the assay, when transformed colonies of cells were becoming apparent, the PDGF-stimulated calcium signal had reduced significantly. In addition, whole-cell patch-clamp electrophysiology measurements indicated a marked reduction in expression of T-type calcium channels. These observations demonstrate that PDGF-induced calcium metabolism changes as initiated cells progress to the transformed phenotype.
Estacion
M
M
Molecular Oncology Program, Cancer Research Center of Hawaii, Honolulu 96813, USA.
Mordan
L J
LJ
D000818:Animals; D002118:Calcium / Q000378:metabolism*; D015220:Calcium Channels; D002461:Cell Line, Transformed; D002471:Cell Transformation, Neoplastic / Q000378:metabolism*; D005347:Fibroblasts / Q000378:metabolism; D016257:Fura-2; D051379:Mice; D018408:Patch-Clamp Techniques; D010982:Platelet-Derived Growth Factor / Q000494:pharmacology*; D015398:Signal Transduction
D015220:Calcium Channels; D010982:Platelet-Derived Growth Factor; D002118:Calcium; D016257:Fura-2