[Effects of NA and 5-HT on the spontaneous and evoked activities of Purkinje cells in cerebellar slice].
Effects of NA and 5-HT perfusion on the spontaneous activity and evoked responses of the Purkinje cells (PCs) to stimulation of white matter were studied in the rat cerebellar slices. The results were as follows: (1) Perfusion with NA could elicit inhibitory, excitatory or biphasic responses of the spontaneously discharging PCs, with inhibitory response in dominance (79.8%). 5-HT perfusion could also exert either excitatory or inhibitory effect on PC spontaneous activity. (2) When NA and 5-HT were perfused successively, NA tended to inhibit while 5-HT tended to excite in 53.8% of the tested cells. (3) Most of the complex spike (CS) and simple spike (SS) responses evoked by white matter stimulation could be potentiated by NA (68.2%, 57.1%) or depressed by 5-HT (60.0%, 68.2%). (4) On the same cell under successively perfusion with NA and 5-HT, the evoked CS and SS responses of most cells (60.0%, 52.9%) showed a tendency to be facilitated by NA and suppressed by 5-HT. These results suggest that aminergic afferent fibers, by releasing NA and 5-HT, may affect PC's excitability and responsiveness to synaptic inputs from mossy and climbing fibers, thus playing an important role in the cerebellar function.
Hu
W H
WH
Department of Biological Science and Technology, Nanjing University.
Wang
J J
JJ
Zhang
M Y
MY
Yu
Q X
QX
D000344:Afferent Pathways; D000818:Animals; D002525:Cerebellar Cortex / Q000502:physiology*; D005071:Evoked Potentials; D009638:Norepinephrine / Q000494:pharmacology*; D011689:Purkinje Cells / Q000502:physiology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D012701:Serotonin / Q000494:pharmacology*
D012701:Serotonin; D009638:Norepinephrine