title: Binding of lectins to novel migration promoters on cardiac mesenchymal cells in the chick.
abstract: Chicken serum promotes migration of cardiac mesenchymal cells of chick embryos in vitro. In the present study, migration promotion of unknown migration promoters in chicken serum was examined by using lectins. Cardiac mesenchymal cells of the conotruncal and atrioventricular cushions were cultured on collagen type-I gel with medium including chicken serum. A concentration (100 micrograms/ml) of Concanavalin A (Con A), peanut agglutinin (PNA), pisum sativum agglutinin (PSA), soybean agglutinin (SBA) or wheat germ agglutinin (WGA) was added to the medium. Con A, PSA, and WGA inhibited migration, while PNA and SBA did not affect migration of cardiac mesenchymal cells. WGA inhibited migration in a concentration dependent manner. Preincubation of WGA with specific binding monosaccharides (alpha-D-N-acetylglucosamine and alpha-D-N-acetylneuraminic acid) clearly reduced the inhibition ability of WGA, while preincubation of Con A and PSA with alpha-D-mannose and alpha-D-glucose did not. On the other hand, Con A-binding proteins, eluted from a Con A affinity column with the buffer including alpha-D-mannose and alpha-D-glucose, promoted migration of cardiac mesenchymal cells, as did WGA-binding proteins. These proteins promoted migration in a concentration dependent manner. Western blotting showed that PSA bound the subunits of collagen type-I, but ConA and WGA did not. In migration inhibition assays by monosaccharides, only N-acetylneuraminic acid inhibited migration of cardiac mesenchymal cells. These results suggested that chicken serum contains novel migration promoters for chick cardiac mesenchymal cells. The promoters are proposed to have the terminal N-acetylglucosamine, N-acetylneuraminic acid, and glucose and/or mannose residues.
authors.affiliation: Department of Anatomy and Physiology, Sanyo Women's College, Hiroshima, Japan. hsumida@mcai.med.hiroshima-u.ac.jp
authors.forename: H
authors.initials: H
authors.lastname: Sumida
authors.forename: H
authors.initials: H
authors.lastname: Nakamura
authors.forename: R P
authors.initials: RP
authors.lastname: Thompson
authors.forename: M
authors.initials: M
authors.lastname: Yasuda
mesh_terms: D000818:Animals; D002465:Cell Movement / Q000187:drug effects; D002478:Cells, Cultured; D002642:Chick Embryo; D003208:Concanavalin A / Q000378:metabolism / Q000494:pharmacology; D037102:Lectins / Q000378:metabolism* / Q000494:pharmacology; D008648:Mesoderm; D009005:Monosaccharides / Q000378:metabolism / Q000494:pharmacology; D009206:Myocardium / Q000166:cytology* / Q000378:metabolism*; D019887:Peanut Agglutinin / Q000378:metabolism / Q000494:pharmacology; D037121:Plant Lectins; D011506:Proteins / Q000032:analysis; D030262:Soybean Proteins; D014909:Wheat Germ Agglutinins / Q000378:metabolism / Q000494:pharmacology; D010835:Phytohemagglutinins
chemical_list: D003208:Concanavalin A; D037102:Lectins; D009005:Monosaccharides; D019887:Peanut Agglutinin; D037121:Plant Lectins; D011506:Proteins; D030262:Soybean Proteins; D014909:Wheat Germ Agglutinins; C036292:pea lectin; C028824:soybean lectin; D010835:Phytohemagglutinins