title: Specificity of the tonoplast transport of the oleanolic acid monoglycosides in the vacuoles from Calendula officinalis leaves.
abstract: The specificity of two separate tonoplast permeases transporting oleanolic acid glycosides was investigated in vacuoles isolated from leaf protoplasts of marigold (Calendula officinalis) with the use of chemically synthesized analogs. The results indicate that the proper structure of both parts of oleanolic acid monoglycoside, i.e. aglycon and the sugar moiety, are required for binding to a specific tonoplast carrier.
authors.affiliation: Institute of Biochemistry, Warsaw University, Poland.
authors.forename: A
authors.initials: A
authors.lastname: Szakiel
authors.forename: W
authors.initials: W
authors.lastname: Janiszowska
mesh_terms: D001692:Biological Transport; D003594:Cytoplasmic Granules / Q000201:enzymology / Q000378:metabolism*; D006027:Glycosides / Q000493:pharmacokinetics; D026901:Membrane Transport Proteins / Q000378:metabolism; D009828:Oleanolic Acid / Q000031:analogs & derivatives / Q000378:metabolism / Q000493:pharmacokinetics*; D018515:Plant Leaves / Q000201:enzymology / Q000378:metabolism; D010940:Plant Proteins / Q000378:metabolism; D010944:Plants / Q000201:enzymology / Q000378:metabolism* / Q000648:ultrastructure; D011523:Protoplasts / Q000378:metabolism; D012680:Sensitivity and Specificity
chemical_list: D006027:Glycosides; D026901:Membrane Transport Proteins; D010940:Plant Proteins; D009828:Oleanolic Acid