title: Streptococcal cell wall-induced arthritis. Requirements for neutrophils, P-selectin, intercellular adhesion molecule-1, and macrophage-inflammatory protein-2.
abstract: Immune arthritis in rat ankle joints was induced by intra-articular injection of streptococcal cell was extract (SCW), followed 21 days later by i.v. injection of SCW. This results in a monoarticular arthritis characterized by an influx of neutrophils and mononuclear cells, a 35-fold increase in urinary excretion of 8-hydroxy-deoxyguanosine (8-OH-dGUA; an index of free radical production), ankle edema, and joint damage/destruction. Neutrophil depletion substantially reduced the intensity of ankle edema. Ab-induced blockade of P-selectin or ICAM-1 also reduced the intensity of ankle edema and the influx of neutrophils. Blockade of TNF-alpha or IL-1 resulted in nearly complete and persistent reduction in ankle edema and profound reductions in the accumulation of neutrophils and mononuclear cells in affected joints. Finally, blocking of macrophage-inflammatory protein-2 reduced ankle edema and neutrophil accumulation during the first 2 days after i.v. challenge with SCW. These data indicate that SCW-induced arthritis is neutrophil dependent and that the recruitment of neutrophils and subsequent joint edema requires ICAM-1, P-selectin, and macrophage-inflammatory protein-2, as well as TNF-alpha and IL-1.
authors.affiliation: Department of Surgery, University of Zurich Medical School, Switzerland.
authors.forename: R C
authors.initials: RC
authors.lastname: Schimmer
authors.forename: D J
authors.initials: DJ
authors.lastname: Schrier
authors.forename: C M
authors.initials: CM
authors.lastname: Flory
authors.forename: J
authors.initials: J
authors.lastname: Dykens
authors.forename: D K
authors.initials: DK
authors.lastname: Tung
authors.forename: P B
authors.initials: PB
authors.lastname: Jacobson
authors.forename: H P
authors.initials: HP
authors.lastname: Friedl
authors.forename: M C
authors.initials: MC
authors.lastname: Conroy
authors.forename: B B
authors.initials: BB
authors.lastname: Schimmer
authors.forename: P A
authors.initials: PA
authors.lastname: Ward
mesh_terms: D000080242:8-Hydroxy-2'-Deoxyguanosine; D000818:Animals; D001168:Arthritis / Q000209:etiology / Q000276:immunology*; D054426:Chemokine CXCL2; D002630:Chemotactic Factors / Q000502:physiology*; D003849:Deoxyguanosine / Q000031:analogs & derivatives / Q000096:biosynthesis / Q000652:urine; D004195:Disease Models, Animal; D004487:Edema / Q000473:pathology; D005260:Female; D007275:Injections, Intravenous; D018799:Intercellular Adhesion Molecule-1 / Q000502:physiology*; D007375:Interleukin-1 / Q000502:physiology; D015846:Monokines / Q000502:physiology*; D009503:Neutropenia / Q000276:immunology; D009504:Neutrophils / Q000502:physiology*; D019007:P-Selectin / Q000502:physiology*; D010457:Peptidoglycan / Q000008:administration & dosage / Q000276:immunology*; D051381:Rats; D011917:Rats, Inbred Lew; D013997:Time Factors; D014409:Tumor Necrosis Factor-alpha / Q000502:physiology
chemical_list: D054426:Chemokine CXCL2; D002630:Chemotactic Factors; D007375:Interleukin-1; D015846:Monokines; D019007:P-Selectin; D010457:Peptidoglycan; D014409:Tumor Necrosis Factor-alpha; D018799:Intercellular Adhesion Molecule-1; D000080242:8-Hydroxy-2'-Deoxyguanosine; D003849:Deoxyguanosine