Viral promoter expression in CEM-C7 and Jurkat human T-lymphoid cell lines.
The strength of various viral promoters was examined in human T lymphoblastoid cell lines. CMV, RSV and SV40 promoter based-luciferase constructs were transiently transfected into CEM-C7 and Jurkat cells in order to compare promoter strength in each cell type. It was found that the CMV promoter was 10-fold stronger than the RSV promoter in Jurkat cells, but equivalent to the RSV promoter in CEM-C7 cells. Both the CMV and RSV promoters were significantly stronger than the SV40 promoter in Jurkat and CEM-C7 cells. In summary, promoter strengths are cell line specific (Jurkat: CMV > RSV >> SV40; CEM-C7: RSV = CMV >> SV40) and care must be exercised in choosing promoters intended to provide overexpression of chimeric genes.
Sutherland
L C
LC
Department of Biological Sciences, Keele University, Staffordshire, UK. bia22@keele.ac.uk
Williams
G T
GT
D001358:Avian Sarcoma Viruses / Q000235:genetics; D002478:Cells, Cultured; D003001:Cloning, Molecular; D003587:Cytomegalovirus / Q000235:genetics; D018274:Electroporation; D015870:Gene Expression; D017930:Genes, Reporter; D005814:Genes, Viral; D006801:Humans; D019169:Jurkat Cells; D011401:Promoter Regions, Genetic / Q000235:genetics*; D013539:Simian virus 40 / Q000235:genetics; D013601:T-Lymphocytes / Q000821:virology*; D014162:Transfection