Relationship of repair and replicative DNA synthesis to cell cycle in Chinese hamster ovary (CHO-K1) cells.
To strengthen the causal association between repair and replicative DNA synthesis, we have simultaneously measured the two types of DNA synthesis in a cell cycle-dependent manner. Synchrony was obtained by counterflow centrifugal elutriation of logarithmic-phase Chinese hamster ovary (CHO) cells kept in suspension cultures. A comparison of cell cycle profiles of ATP-dependent replicative and ATP-independent repair synthesis in permeable cells shows opposite trends. The rates of repair synthesis and replication are inversely correlated.
Banfalvi
G
G
Department of Medical Chemistry, Molecular Biology and Pathobiochemistry Semmelweis University Medical School, Budapest, Hungary.
Poirier
L A
LA
Mikhailova
M
M
Chou
M W
MW
D000255:Adenosine Triphosphate / Q000502:physiology; D000818:Animals; D016590:Aphidicolin / Q000494:pharmacology; D016466:CHO Cells; D002453:Cell Cycle / Q000235:genetics*; D002463:Cell Membrane Permeability; D006224:Cricetinae; D004260:DNA Repair / Q000235:genetics*; D004261:DNA Replication / Q000235:genetics*; D054306:Dideoxynucleotides; D004791:Enzyme Inhibitors / Q000494:pharmacology; D019384:Nucleic Acid Synthesis Inhibitors; D013942:Thymine Nucleotides / Q000494:pharmacology
D054306:Dideoxynucleotides; D004791:Enzyme Inhibitors; D019384:Nucleic Acid Synthesis Inhibitors; D013942:Thymine Nucleotides; D016590:Aphidicolin; D000255:Adenosine Triphosphate; C017216:2',3'-dideoxythymidine triphosphate