title: Effect of microencapsulation on immunogenicity of a bovine herpes virus glycoprotein and inactivated influenza virus in mice.
abstract: We previously found that aqueous-based spermine-alginate or spermine-chondroitin sulfate microcapsules enhanced rotavirus-specific humoral immune responses after intramuscular inoculation of mice. To extend our observations with whole, infectious rotavirus to vaccine strategies which include inactivated virus and purified proteins, we determined the capacity of aqueous-based microcapsules to enhance virus-specific immune responses to bovine herpes virus type 1 glycoprotein D (BHV-1-gD) or ether-treated influenza virus. We found that spermine-alginate microcapsules decreased the quantity of BHV-1-gD necessary to induce protein-specific antibodies about 5000-fold. However, spermine-alginate microcapsules did not enhance influenza virus-specific antibody responses. Microcapsules composed of spermine-chondroitin sulfate did not enhance either BHV-1-gD or influenza virus-specific immune responses. Possible mechanisms of enhancement of virus-specific antibody responses by microencapsulation are discussed.
authors.affiliation: Section of Infectious Diseases, Children's Hospital of Philadelphia, PA 19104, USA.
authors.forename: C A
authors.initials: CA
authors.lastname: Moser
authors.forename: T J
authors.initials: TJ
authors.lastname: Speaker
authors.forename: P A
authors.initials: PA
authors.lastname: Offit
mesh_terms: D000464:Alginates / Q000008:administration & dosage; D000818:Animals; D000914:Antibodies, Viral / Q000008:administration & dosage / Q000096:biosynthesis / Q000276:immunology; D000918:Antibody Specificity; D000956:Antigens, Viral / Q000276:immunology; D002214:Capsules; D002417:Cattle; D002642:Chick Embryo; D002645:Chickens; D004339:Drug Compounding; D004355:Drug Stability; D005260:Female; D020723:Glucuronic Acid; D019267:Hemagglutinin Glycoproteins, Influenza Virus / Q000276:immunology; D022283:Herpesvirus Vaccines; D006603:Hexuronic Acids; D009980:Influenza A virus / Q000276:immunology*; D007252:Influenza Vaccines / Q000008:administration & dosage* / Q000276:immunology*; D051379:Mice; D013096:Spermine / Q000008:administration & dosage; D015164:Vaccines, Inactivated / Q000008:administration & dosage / Q000276:immunology; D014759:Viral Envelope Proteins / Q000008:administration & dosage* / Q000276:immunology*; D014765:Viral Vaccines / Q000008:administration & dosage* / Q000276:immunology*
chemical_list: D000464:Alginates; D000914:Antibodies, Viral; D000956:Antigens, Viral; D002214:Capsules; D019267:Hemagglutinin Glycoproteins, Influenza Virus; D022283:Herpesvirus Vaccines; D006603:Hexuronic Acids; D007252:Influenza Vaccines; D015164:Vaccines, Inactivated; D014759:Viral Envelope Proteins; D014765:Viral Vaccines; C039447:glycoprotein D, Human herpesvirus 1; C069093:herpes simplex virus-1 glycoprotein D vaccine; D013096:Spermine; D020723:Glucuronic Acid