Different serological examinations in cystic fibrosis patients: response to Pseudomonas aeruginosa St-Ag.
Crossed immunoelectrophoresis (CIE), enzyme linked immunosorbent assay (ELISA) and immunoblotting (IMB) were used to look for the presence of anti-bodies to Pseudomonas aeruginosa standard antigen in the sera of cystic fibrosis patients with intermittent (CF +/- P) and chronic (CF + P) colonization. Our results show that CIE is too complex a method to use when monitoring the infection over time, that with ELISA a correlation with the patient's clinical status may be made and that IMB can evidence an early, albeit low, reaction in the CF +/- P patient.
Magni
A
A
Institute of Microbiology, Medical School, La Sapienza University of Rome, Italy.
Trancassini
M
M
Giordano
A
A
De Vito
D
D
Cimino
G
G
Quattrucci
S
S
Antonelli
M
M
Cipriani
P
P
D000328:Adult; D000907:Antibodies, Bacterial / Q000096:biosynthesis / Q000097:blood; D000942:Antigens, Bacterial / Q000276:immunology*; D002675:Child, Preschool; D002908:Chronic Disease; D003550:Cystic Fibrosis / Q000097:blood / Q000276:immunology* / Q000382:microbiology*; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D015151:Immunoblotting; D007123:Immunoelectrophoresis, Two-Dimensional; D011550:Pseudomonas aeruginosa / Q000276:immunology*
D000907:Antibodies, Bacterial; D000942:Antigens, Bacterial