E- and P-selectin are not involved in the recruitment of inflammatory cells across the blood-brain barrier in experimental autoimmune encephalomyelitis.
In experimental autoimmune encephalomyelitis (EAE) inflammatory cells cross the endothelial blood-brain barrier (BBB) and gain access to the central nervous system (CNS). Here we show that E- and P-selectin are not involved in the recruitment of inflammatory cells across the BBB. Neither expression of E- nor P-selectin is induced in BBB-forming endothelium at any time after initiation of EAE. Some of the inflammatory cells present in the CNS during EAE express ligands for E- or P-selectin. However, anti-E- and P-selectin antibodies influence neither immigration of inflammatory cells across the BBB nor the development of EAE. In general, suppression of E- and P-selectin expression on BBB endothelium is dependent on factors derived from the CNS microenvironment, eg, astrocytes. Our results suggest that during EAE suppression of E- and P-selectin expression on the BBB provides a CNS-specific mechanism to reduce leukocyte recruitment into the CNS.
Engelhardt
B
B
Max-Planck Institut für physiologische und klinische Forschung, W. G. Kerckhoff-Institut, Bad Nauheim, Germany.
Vestweber
D
D
Hallmann
R
R
Schulz
M
M
D000818:Animals; D000911:Antibodies, Monoclonal; D001812:Blood-Brain Barrier; D002196:Capillaries / Q000473:pathology; D002478:Cells, Cultured; D002560:Cerebrovascular Circulation; D019040:E-Selectin / Q000032:analysis / Q000502:physiology*; D004681:Encephalomyelitis, Autoimmune, Experimental / Q000473:pathology*; D004730:Endothelium, Vascular / Q000473:pathology; D005260:Female; D007107:Immune System / Q000473:pathology*; D008198:Lymph Nodes / Q000737:chemistry; D051379:Mice; D008815:Mice, Inbred Strains; D019007:P-Selectin / Q000032:analysis / Q000502:physiology*; D012333:RNA, Messenger / Q000378:metabolism; D019010:Vascular Cell Adhesion Molecule-1 / Q000032:analysis
D000911:Antibodies, Monoclonal; D019040:E-Selectin; D019007:P-Selectin; D012333:RNA, Messenger; D019010:Vascular Cell Adhesion Molecule-1