Immunostimulatory activities of mono- or diglycosylated alpha-galactosylceramides.
We examined the effects of 2"- or 3"-monoglycosylated alpha-galactosylceramides (alpha-GalCers) and 2",3"-diglycosylated alpha-GalCers on allogeneic MLR and the proliferation of murine spleen cells. It was found that their ceramide portions greatly affect their immunostimulatory activities, and that the 3"-hydroxyl group plays a more important role in the immunostimulatory effects of alpha-GalCer derivatives than the 2"-hydroxyl group.
Uchimura
A
A
Pharmaceutical Research Laboratory, Kirin Brewery Co., Ltd, Gunma, Japan.
Shimizu
T
T
Nakajima
M
M
Ueno
H
H
Motoki
K
K
Fukushima
H
H
Natori
T
T
Koezuka
Y
Y
D000276:Adjuvants, Immunologic / Q000494:pharmacology*; D000818:Animals; D002240:Carbohydrate Sequence; D005260:Female; D005699:Galactosylceramides / Q000494:pharmacology*; D006031:Glycosylation; D008198:Lymph Nodes / Q000166:cytology / Q000187:drug effects; D008213:Lymphocyte Activation / Q000187:drug effects; D007959:Lymphocyte Culture Test, Mixed; D008214:Lymphocytes / Q000187:drug effects / Q000276:immunology; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D008969:Molecular Sequence Data; D013154:Spleen / Q000166:cytology / Q000187:drug effects
D000276:Adjuvants, Immunologic; D005699:Galactosylceramides