title: Physiological and biochemical characterization of intergeneric hybrids of thermotolerant and non-thermotolerant yeasts.
abstract: Kluyveromyces-like intergeneric hybrids of thermotolerant Kluyveromyces marxianus and non-thermotolerant Saccharomyces cerevisiae, produced in a previous study by protoplasmic fusion, have been characterized. On molasses, these strains produced ethanol in excess of 6% (v/v) both at 30 and 45 degrees C as against 3% and 4.2% (v/v) by the former parent at 30 and 45 degrees C, respectively. In hybrids, the increase in ethanol production appeared to be a sequel to increased activities of alcohol dehydrogenase and pyruvate kinase, derived probably from S. cerevisiae parent. Hybrid ADH-isozyme pattern on polyacrylamide gel corroborated the presence of S. cerevisiae ADH in the tested hybrids. Regression analyses indicated a positive correlation between ethanol production and ADH or PK or both (r approximately 0.76-0.84).
authors.affiliation: Department of Microbiology, CCS Haryana Agricultural University, Hisar, India.
authors.forename: S S
authors.initials: SS
authors.lastname: Dhamija
authors.forename: R
authors.initials: R
authors.lastname: Gera
authors.forename: D K
authors.initials: DK
authors.lastname: Singh
authors.forename: D
authors.initials: D
authors.lastname: Singh
authors.forename: R
authors.initials: R
authors.lastname: Marchant
mesh_terms: D006824:Hybridization, Genetic; D007716:Kluyveromyces / Q000235:genetics / Q000502:physiology*; D012441:Saccharomyces cerevisiae / Q000235:genetics / Q000502:physiology*