c-di-GMP-binding protein, a new factor regulating cellulose synthesis in Acetobacter xylinum.
A protein which specifically binds cyclic diguanylic acid (c-di-GMP), the reversible allosteric activator of the membrane-bound cellulose synthase system of Acetobacter xylinum, has been identified in membrane preparations of this organism. c-di-GMP binding is of high affinity (KD 20 nM), saturable and reversible. The equilibrium of the reaction is markedly and specifically shifted towards the binding direction by K+. The c-di-GMP binding protein, structurally associated with the cellulose synthase, appears to play a major role in modulating the intracellular concentration of free c-di-GMP and thus may constitute an essential factor in regulating cellulose synthesis in vivo.
Weinhouse
H
H
Department of Biological Chemistry, Institute of Life Sciences, Hebrew University of Jerusalem, Givat Ram, Israel.
Sapir
S
S
Amikam
D
D
Shilo
Y
Y
Volman
G
G
Ohana
P
P
Benziman
M
M
D000494:Allosteric Regulation; D001426:Bacterial Proteins / Q000302:isolation & purification / Q000378:metabolism*; D002352:Carrier Proteins / Q000302:isolation & purification / Q000378:metabolism*; D002482:Cellulose / Q000096:biosynthesis*; D002850:Chromatography, Gel; D006152:Cyclic GMP / Q000031:analogs & derivatives* / Q000378:metabolism; D004734:Energy Metabolism / Q000187:drug effects; D004789:Enzyme Activation; D004983:Ethanolamines / Q000494:pharmacology; D041822:Gluconacetobacter xylinus / Q000378:metabolism*; D005964:Glucosyltransferases / Q000378:metabolism; D007700:Kinetics; D011188:Potassium / Q000494:pharmacology
D001426:Bacterial Proteins; D002352:Carrier Proteins; D004983:Ethanolamines; C062025:bis(3',5')-cyclic diguanylic acid; D002482:Cellulose; C020283:diethanolamine; D005964:Glucosyltransferases; C076062:cellulose synthase (cyclic diguanylic acid); D006152:Cyclic GMP; D011188:Potassium