Head-to-tail primer tandem repeats generated in hemi-nested PCR.
Evidence that hemi-nested PCR protocols are prone to the development of artifacts consisting of multiple head-to-tail primer tandem repeats is presented. This phenomenon was more pronounced with the addition of a large amount of template into the second round PCR and was limited to the primer used in both first and second reactions.
Wang
L F
LF
Division of Transplant Medicine, Pittsburgh Transplantation Institute, PA 15213, USA.
Rakela
J
J
Laskus
T
T
D016477:Artifacts; D003001:Cloning, Molecular; D017931:DNA Primers / Q000737:chemistry / Q000235:genetics; D004587:Electrophoresis, Agar Gel; D006515:Hepatitis B virus / Q000235:genetics*; D016133:Polymerase Chain Reaction; D012091:Repetitive Sequences, Nucleic Acid
D017931:DNA Primers