title: The presence of arginine vasopressin and its mRNA in rat choroid plexus epithelium.
abstract: Arginine vasopressin (AVP) plays an important role in the regulation of secretory function and hemodynamics of choroid plexus, the primary site of cerebrospinal fluid (CSF) production. In the present study, localization of AVP and its transcripts in choroid plexus of adult male Sprague-Dawley rats was studied by immunohistochemistry and in situ hybridization histochemistry, respectively. For immunohistochemical analysis, AVP-specific polyclonal rabbit antibody was employed. Plasmid, pGrVP, containing a 232-bp fragment of rat AVP cDNA encoding the C-terminus of proAVP, was used as a probe to detect AVP mRNA. AVP-immunoreactive product was predominantly localized close to the apical (CSF-facing) membrane of choroidal epithelium while AVP transcripts were distributed throughout the cytoplasm of the cells. Our findings indicate that AVP is synthesized in choroid plexus epithelium, which suggests autocrine and/or paracrine actions of this peptide in choroidal tissue.
authors.affiliation: Department of Clinical Neurosciences, Brown University/Rhode Island Hospital, Providence 02903, USA.
authors.forename: A
authors.initials: A
authors.lastname: Chodobski
authors.forename: Y P
authors.initials: YP
authors.lastname: Loh
authors.forename: S
authors.initials: S
authors.lastname: Corsetti
authors.forename: J
authors.initials: J
authors.lastname: Szmydynger-Chodobska
authors.forename: C E
authors.initials: CE
authors.lastname: Johanson
authors.forename: Y P
authors.initials: YP
authors.lastname: Lim
authors.forename: P R
authors.initials: PR
authors.lastname: Monfils
mesh_terms: D000818:Animals; D000906:Antibodies; D001127:Arginine Vasopressin / Q000032:analysis* / Q000096:biosynthesis*; D002831:Choroid Plexus / Q000166:cytology / Q000378:metabolism*; D004847:Epithelial Cells / Q000378:metabolism*; D007150:Immunohistochemistry; D017403:In Situ Hybridization; D008297:Male; D015345:Oligonucleotide Probes; D012333:RNA, Messenger / Q000032:analysis* / Q000096:biosynthesis; D011817:Rabbits; D051381:Rats; D017207:Rats, Sprague-Dawley; D014158:Transcription, Genetic
chemical_list: D000906:Antibodies; D015345:Oligonucleotide Probes; D012333:RNA, Messenger; D001127:Arginine Vasopressin