Yeast microarrays for genome wide parallel genetic and gene expression analysis.
We have developed high-density DNA microarrays of yeast ORFs. These microarrays can monitor hybridization to ORFs for applications such as quantitative differential gene expression analysis and screening for sequence polymorphisms. Automated scripts retrieved sequence information from public databases to locate predicted ORFs and select appropriate primers for amplification. The primers were used to amplify yeast ORFs in 96-well plates, and the resulting products were arrayed using an automated micro arraying device. Arrays containing up to 2,479 yeast ORFs were printed on a single slide. The hybridization of fluorescently labeled samples to the array were detected and quantitated with a laser confocal scanning microscope. Applications of the microarrays are shown for genetic and gene expression analysis at the whole genome level.
Lashkari
D A
DA
Department of Genetics, Stanford University, CA 94305, USA.
DeRisi
J L
JL
McCusker
J H
JH
Namath
A F
AF
Gentile
C
C
Hwang
S Y
SY
Brown
P O
PO
Davis
R W
RW
D003080:Cold Temperature; D017931:DNA Primers; D018076:DNA, Complementary; D005690:Galactose / Q000378:metabolism; D015870:Gene Expression; D016681:Genome, Fungal; D005838:Genotype; D005947:Glucose / Q000378:metabolism; D018869:Heat-Shock Response; D016366:Open Reading Frames; D012441:Saccharomyces cerevisiae / Q000235:genetics* / Q000378:metabolism
D017931:DNA Primers; D018076:DNA, Complementary; D005947:Glucose; D005690:Galactose