Protective antibodies and anamnestic response in Salvelinus fontinalis to Cryptobia salmositica and innate resistance of Salvelinus namaycush to the hemoflagellate.
Cryptobia-susceptible Salvelinus fontinalis vaccinated with a live Cryptobia salmositica vaccine were protected against C. salmositica and they were still protected 53 wk after their initial challenge. Parasites were lysed when they were incubated with immune plasma and complement and this confirms that complement-fixing antibody is an important part of the protective mechanism. Immunological memory was demonstrated in both vaccinated-challenged, and infected-recovered S. fontinalis as there were rapid and significant increases in complement-fixing antibody titers after parasite challenge. This indicates that S. fontinalis are capable of an anamnestic response. Most naive Salvelinus namaycush were not susceptible to C. salmositica infection, and their fresh plasma lysed C. salmositica under in vitro conditions. The lytic factor(s) was inactivated by heating the plasma prior to parasite incubation. This indicates that the alternate pathway of complement activation is likely the mechanism of innate resistance in S. namaycush.
Ardelli
B F
BF
Department of Zoology, University of Guelph, Ontario, Canada.
Woo
P T
PT
D000818:Animals; D000913:Antibodies, Protozoan / Q000096:biosynthesis / Q000097:blood; D003168:Complement Fixation Tests / Q000662:veterinary; D003170:Complement Pathway, Alternative; D056890:Eukaryota / Q000276:immunology* / Q000472:pathogenicity; D005260:Female; D005393:Fish Diseases / Q000276:immunology / Q000517:prevention & control*; D007110:Immunity, Active; D007113:Immunity, Innate; D007156:Immunologic Memory; D008297:Male; D018512:Parasitemia / Q000276:immunology / Q000517:prevention & control / Q000662:veterinary*; D011528:Protozoan Infections / Q000276:immunology / Q000517:prevention & control; D011529:Protozoan Infections, Animal; D016052:Protozoan Vaccines / Q000276:immunology; D014337:Trout / Q000469:parasitology*; D014611:Vaccination / Q000662:veterinary; D014613:Vaccines, Attenuated / Q000276:immunology; D014774:Virulence
D000913:Antibodies, Protozoan; D016052:Protozoan Vaccines; D014613:Vaccines, Attenuated