title: Evidence for oxidative stress in the hepatic mitochondria of bile duct ligated rats.
abstract: Lipid peroxidation increased both in the liver homogenates and in the hepatic mitochondrial fraction of bile duct ligated (BDL)-rats. Although mitochondrial superoxide dismutase (SOD) activity did not change in the liver, glutathione (GSH) levels and glutathione peroxidase (GSH-Px) activity decreased in hepatic mitochondrial fraction of BDL-rats as compared to sham-operated rats. In addition, GSH-Px and glutathione transferase (GST) activities decreased but SOD activity remained unchanged in the post-mitochondrial fraction of the liver from BDL-rats. However, erythrocyte lipid peroxide and GSH levels did not change in BDL-rats. In conclusion, our results show that the disturbance of oxidant-antioxidant balance especially in mitochondria may be responsible for cholestatic liver injury in BDL-rats.
authors.affiliation: Department of Biochemistry, Istanbul Faculty of Medicine, University of Istanbul, Istanbul, 34390 Capa, Turkey.
authors.forename: N
authors.initials: N
authors.lastname: Alptekin
authors.forename: G
authors.initials: G
authors.lastname: Mehmetçik
authors.forename: M
authors.initials: M
authors.lastname: Uysal
authors.forename: G
authors.initials: G
authors.lastname: Aykaç-toker
mesh_terms: D000818:Animals; D002779:Cholestasis / Q000378:metabolism*; D004912:Erythrocytes / Q000378:metabolism; D005978:Glutathione / Q000378:metabolism; D005979:Glutathione Peroxidase / Q000378:metabolism; D015227:Lipid Peroxidation / Q000502:physiology; D008099:Liver / Q000378:metabolism*; D008297:Male; D008930:Mitochondria, Liver / Q000378:metabolism*; D018384:Oxidative Stress / Q000502:physiology*; D051381:Rats; D017208:Rats, Wistar; D013482:Superoxide Dismutase / Q000378:metabolism
chemical_list: D005979:Glutathione Peroxidase; D013482:Superoxide Dismutase; D005978:Glutathione