HIV and SIV envelope glycoproteins induce phospholipase A2 activation in human and macaque lymphocytes.
We investigated the early interactions between HIV-1, HIV-2, and simian immunodeficiency virus (SIV) envelope glycoproteins gp120(IIIB), gp105(ROD), and gp120(mac251), and human and macaque cells of the lymphocytic series. Our results demonstrate that the soluble viral glycoproteins induce a specific phospholipase A2 (PLA2) activation in lymphocytes through CD4. This PLA2 activation was induced after envelope glycoprotein-CD4 interaction and, because of its local membrane-destabilizing effect, may have important implications for preparing the lymphocyte membrane for fusion with the viral particle. However, this effect is not sufficient to accomplish fusion. These data indicate that the specific step of fusion may be downstream from PLA2 activation.
Mavoungou
E
E
Department of Immunology and Immunogenetics, International Center for Medical Research, Franceville, Gabon. emavoung@cirmfrv.fr
Georges-Courbot
M C
MC
Poaty-Mavoungou
V
V
Nguyen
H T
HT
Yaba
P
P
Delicat
A
A
Georges
A J
AJ
Russo-Marie
F
F
D000818:Animals; D016718:Arachidonic Acid / Q000378:metabolism; D001896:Boron Compounds; D015496:CD4-Positive T-Lymphocytes / Q000187:drug effects / Q000201:enzymology*; D004789:Enzyme Activation; D005260:Female; D005434:Flow Cytometry; D005456:Fluorescent Dyes; D015686:Gene Products, env / Q000494:pharmacology*; D015699:HIV Envelope Protein gp120 / Q000494:pharmacology*; D015497:HIV-1 / Q000737:chemistry*; D015498:HIV-2 / Q000737:chemistry*; D006801:Humans; D008081:Liposomes; D008213:Lymphocyte Activation / Q000187:drug effects; D008252:Macaca fascicularis; D008297:Male; D010713:Phosphatidylcholines; D010741:Phospholipases A / Q000378:metabolism*; D054467:Phospholipases A2; D015302:Simian Immunodeficiency Virus / Q000737:chemistry*; D054299:env Gene Products, Human Immunodeficiency Virus
C095489:4,4-difluoro-4-bora-3a,4a-diaza-s-indacene; D001896:Boron Compounds; D005456:Fluorescent Dyes; D015686:Gene Products, env; D015699:HIV Envelope Protein gp120; D008081:Liposomes; D010713:Phosphatidylcholines; D054299:env Gene Products, Human Immunodeficiency Virus; C069790:gp105 envelope protein, Human immunodeficiency virus 2; D016718:Arachidonic Acid; D010741:Phospholipases A; D054467:Phospholipases A2