[Localization of PTH gene on pig chromosome 14q25-q28 by in situ hybridization].
The human parathyroid hormone (PTH) genic DNA, as a probe, was labelled with tritium(3H) using nick translation and random oligonucleotide primers methods. Metaphase chromosomal G-bands were prepared from cultured blood lymphocytes of domestic pig. After in situ hybridization and autoradiographly, the number of silver grains on every chromosome was calculated. The result showed that PTH gene is located at chromosome 14q25-q28. The relevent points of this study are discussed.
Ji
Q
Q
Department of Biolohy Nanjing Normal University.
Zhang
X
X
Chen
Y
Y
D000818:Animals; D002874:Chromosome Mapping; D006801:Humans; D017403:In Situ Hybridization; D010281:Parathyroid Hormone / Q000235:genetics*; D013552:Swine
D010281:Parathyroid Hormone