title: The phylogenetic position of the Thermococcus isolate AN1 based on 16S rRNA gene sequence analysis: a proposal that AN1 represents a new species, Thermococcus zilligii sp. nov.
abstract: The 16S rRNA gene from the Thermococcus New Zealand isolate AN1 was cloned and sequenced. Analysis of the gene revealed the presence of signature sequences, indicating that strain AN1 represents a new species of the genus Thermococcus. Since the isolate AN1 differed from other thermococci in both its lower optimal NaCl concentration and generally lower optimal temperature for growth, in its unusual lipid membrane composition, and in its sensitivity to antibiotics, we propose that strain AN1 represents a new species of Thermococcus. The proposed name is Thermococcus zilligii, and the type strain is DSM 2770.
authors.affiliation: Thermophile Research Unit, University of Waikato, Private Bag 3105, Hamilton, New Zealand. ronimus@waikoato.ac.nz
authors.forename: R S
authors.initials: RS
authors.lastname: Ronimus
authors.forename: A
authors.initials: A
authors.lastname: Reysenbach
authors.forename: D R
authors.initials: DR
authors.lastname: Musgrave
authors.forename: H W
authors.initials: HW
authors.lastname: Morgan
mesh_terms: D001105:Archaea / Q000145:classification* / Q000235:genetics*; D015373:Bacterial Typing Techniques; D003001:Cloning, Molecular; D004275:DNA, Ribosomal / Q000235:genetics*; D008969:Molecular Sequence Data; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D012336:RNA, Ribosomal, 16S / Q000235:genetics*; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA
chemical_list: D004275:DNA, Ribosomal; D012336:RNA, Ribosomal, 16S