title: Use of dexamethasone with TTX block of nerve conduction shows that muscle membrane properties are fully controlled by evoked activity.
abstract: This paper provides further evidence that motorneurons control extrajunctional properties of skeletal muscles through the activity evoked in the muscle fibres. The experiments compare the amount of action potential resistance to tetrodotoxin (TTX resistance) in denervated soleus muscle with that in soleus whose nerve was crushed and then allowed to regenerate in the presence of a block of the sciatic impulse conduction. Measurements were taken after about 2-3 weeks to allow full reinnervation and recovery of trophic regulation by the nerve. Blocking sciatic impulse conduction with TTX solutions containing low doses of the anti-inflammatory drug dexamethasone induced values of extrajunctional TTX resistance identical to those caused by denervation. In contrast lower levels of TTX resistance were obtained with dexamethasone-free solutions or when the drug was administered through the systemic path rather than topically applied to the nerve. These results indicate that physiological neural regulatory signals other than activity do not participate to the regulation of extrajunctional properties of skeletal muscles. Furthermore the low levels of TTX resistance measured with dexamethasone-free blocks confirm our previous experiments indicating that reported differences between denervation and pure inactivity are attributable to incomplete suppression of nerve impulse conduction.
authors.affiliation: Dipartimento di Scienze Neurologiche e della Visione, University of Verona, Italy. pasino@borgoroma.univr.it
authors.forename: E
authors.initials: E
authors.lastname: Pasino
authors.forename: M
authors.initials: M
authors.lastname: Buffelli
authors.forename: G
authors.initials: G
authors.lastname: Busetto
authors.forename: A
authors.initials: A
authors.lastname: Cangiano
mesh_terms: D000818:Animals; D000893:Anti-Inflammatory Agents / Q000494:pharmacology*; D002462:Cell Membrane / Q000737:chemistry / Q000502:physiology; D003907:Dexamethasone / Q000494:pharmacology*; D004359:Drug Therapy, Combination; D019054:Evoked Potentials, Motor / Q000502:physiology; D005547:Foreign Bodies / Q000503:physiopathology; D008297:Male; D009121:Muscle Denervation; D018482:Muscle, Skeletal / Q000737:chemistry / Q000294:innervation / Q000502:physiology*; D009407:Nerve Block; D009431:Neural Conduction / Q000187:drug effects*; D051381:Rats; D017208:Rats, Wistar; D012584:Sciatic Nerve / Q000187:drug effects / Q000502:physiology; D015222:Sodium Channels / Q000187:drug effects / Q000502:physiology; D013779:Tetrodotoxin / Q000494:pharmacology*
chemical_list: D000893:Anti-Inflammatory Agents; D015222:Sodium Channels; D013779:Tetrodotoxin; D003907:Dexamethasone