Ca2+/Mg(2+)-dependent endonuclease in marrow CD34 positive and erythroid cells in myelodysplasia.
Endonucleases capable of producing internucleosomal DNA cleavage are one of the key enzymes in apoptosis. We examined endonuclease activities contained in nuclei of CD34+ and erythroid cells in the bone marrow (BM) from 12 patients with the myelodysplastic syndromes. The levels of Mg(2+)-dependent and acidic endonucleases showed little changes as compared with those from normal BM. By contrast, the level of Ca2+/Mg(2+)-dependent endonuclease was appreciably higher in MDS erythroid cells than normal counterparts, although the activity varied markedly in CD34+ and erythroid cells. Our results suggested that Ca2+/Mg(2+)-dependent endonuclease is related to ineffective erythropoiesis in MDS.
Anzai
N
N
Department of Hematology and Oncology, Graduate School of Medicine, Kyoto University, Japan.
Kawabata
H
H
Hishita
T
T
Yoshida
Y
Y
Ueda
Y
Y
Okuma
M
M
D000328:Adult; D000368:Aged; D018952:Antigens, CD34 / Q000032:analysis*; D004706:Endodeoxyribonucleases / Q000378:metabolism*; D015672:Erythroid Precursor Cells / Q000201:enzymology*; D005260:Female; D006412:Hematopoietic Stem Cells / Q000201:enzymology*; D006801:Humans; D008297:Male; D008875:Middle Aged; D009190:Myelodysplastic Syndromes / Q000097:blood / Q000201:enzymology*
D018952:Antigens, CD34; D004706:Endodeoxyribonucleases; C029367:calcium magnesium dependent endodeoxyribonuclease