Identification of Chlorella T-1 ingredient which enhances DMPO-OH adduct formation in Fenton reaction.
Chlorella T-1 is a micro algae which carries out highly active photosynthetic carbon fixation. For searching the OH scavenging molecules in this strain, the methanol extract of T-1 was fractionated by RP-HPLC and the fractions were analyzed for DMPO-OH formation by ESR after Fenton reaction. In the course of study, a single chlorella T-1 ingredient was found to enhance the formation of DMPO-OH rather than scavenge in the Fenton system. The OH enhancing chlorella component was further purified by chromatography and finally identified as lactic acid by NMR and LC-MS. From the analysis of the reaction conditions for DMPO-OH, it was found that lactic acid did not serve as direct source of OH but accelerated Fenton reaction.
Ali
M A
MA
Department of Radiochemistry-Biophysics, Niigata College of Pharmacy, Japan.
Oykawa
K
K
Ohwada
M
M
Konishi
T
T
D002708:Chlorella / Q000378:metabolism*; D003497:Cyclic N-Oxides / Q000378:metabolism*; D004578:Electron Spin Resonance Spectroscopy; D016166:Free Radical Scavengers / Q000378:metabolism; D006861:Hydrogen Peroxide; D017665:Hydroxyl Radical / Q000378:metabolism*; D007501:Iron; D019344:Lactic Acid / Q000302:isolation & purification / Q000378:metabolism*; D010788:Photosynthesis; D013113:Spin Labels
D003497:Cyclic N-Oxides; C045076:Fenton's reagent; D016166:Free Radical Scavengers; D013113:Spin Labels; D017665:Hydroxyl Radical; D019344:Lactic Acid; C069977:2,2-dimethyl-5-hydroxy-1-pyrrolidinyloxy; D006861:Hydrogen Peroxide; D007501:Iron