Opioid regulation of AP-1 DNA-binding activity in NG108-15 cells under conditions of opioid-receptor adaptation.
Opioid-receptor adaptation may lead to changes in transcriptional regulation by sequence-specific DNA-binding proteins. Gel-shift assays of nuclear extracts from NG108-15 cells revealed that an increase of AP-1 DNA-binding activity ensues under conditions previously established to induce down- or up-regulation of delta-opioid receptors.
Tencheva
Z S
ZS
Institute of Physiology, Bulgarian Academy of Science, Sofia.
Belcheva
M M
MM
Velichkova
A A
AA
Lissichkova
E G
EG
Coscia
C J
CJ
D000818:Animals; D001483:Base Sequence; D001665:Binding Sites; D002467:Cell Nucleus / Q000187:drug effects / Q000378:metabolism; D016384:Consensus Sequence; D015536:Down-Regulation / Q000187:drug effects; D016308:Enkephalin, Leucine-2-Alanine / Q000494:pharmacology*; D005910:Glioma; D006822:Hybrid Cells; D051379:Mice; D009271:Naltrexone / Q000494:pharmacology*; D009447:Neuroblastoma; D009838:Oligodeoxyribonucleotides / Q000737:chemistry / Q000378:metabolism*; D051381:Rats; D017465:Receptors, Opioid, delta / Q000096:biosynthesis*; D018808:Transcription Factor AP-1 / Q000378:metabolism*; D014407:Tumor Cells, Cultured; D015854:Up-Regulation / Q000187:drug effects
D009838:Oligodeoxyribonucleotides; D017465:Receptors, Opioid, delta; D018808:Transcription Factor AP-1; D009271:Naltrexone; D016308:Enkephalin, Leucine-2-Alanine