title: Isolation and some properties of sorbitol oxidase from Streptomyces sp. H-7775.
abstract: A sorbitol oxidase (SOX) was found in the cell-free extract of a strain isolated from soil. The strain was classified and designated as Streptomyces sp. H-7775. SOX is constitutively expressed in the cell. The molecular weight of SOX that purified from the cell-free extract was 45,000. The optimum pH and the K(m) for sorbitol were 6.5-7.5 and 0.26 mM, respectively. The prosthetic group was a covalently bound FAD. SOX catalyzed oxidation of D-sorbitol to glucose and hydrogen peroxide without any requirements of exogenous cofactors. SOX did not react with glucose, a reaction product of D-sorbitol. This feature is useful in its application for diagnosis.
authors.affiliation: Department of Applied Biology, Faculty of Textile Science, Kyoto Institute of Technology, Japan.
authors.forename: K
authors.initials: K
authors.lastname: Hiraga
authors.forename: M
authors.initials: M
authors.lastname: Kitazawa
authors.forename: N
authors.initials: N
authors.lastname: Kaneko
authors.forename: K
authors.initials: K
authors.lastname: Oda
mesh_terms: D002846:Chromatography, Affinity; D002855:Chromatography, Thin Layer; D004591:Electrophoresis, Polyacrylamide Gel; D004795:Enzyme Stability; D007124:Immunoenzyme Techniques; D009682:Magnetic Resonance Spectroscopy; D010088:Oxidoreductases / Q000302:isolation & purification* / Q000378:metabolism; D013302:Streptomyces / Q000201:enzymology*
chemical_list: D010088:Oxidoreductases; C111983:sorbitol oxidase