title: Microsatellite-based cancer detection using capillary array electrophoresis and energy-transfer fluorescent primers.
abstract: The development of sensitive, rapid, and accurate methods and apparatus for high-throughput short tandem repeat (STR) analysis will be critical for the use of microsatellite alteration in cancer screening. Here we show that STR-based bladder cancer diagnosis can be performed using capillary array electrophoresis and two-color labeling with energy-transfer (ET) fluorescent primers. Rapid (< or = 35 min) separations are achieved on capillary arrays using replaceable separation matrices and the allelic ratios are quantitatively determined with a precision of +/- 10%. With this precision, a variation of 20% was considered diagnostically significant. These methods provide a significant improvement in the speed, ease, and precision of STR analyses compared to slab gel electrophoresis.
authors.affiliation: Department of Chemistry, University of California, Berkeley 94720, USA.
authors.forename: Y
authors.initials: Y
authors.lastname: Wang
authors.forename: S C
authors.initials: SC
authors.lastname: Hung
authors.forename: J F
authors.initials: JF
authors.lastname: Linn
authors.forename: G
authors.initials: G
authors.lastname: Steiner
authors.forename: A N
authors.initials: AN
authors.lastname: Glazer
authors.forename: D
authors.initials: D
authors.lastname: Sidransky
authors.forename: R A
authors.initials: RA
authors.lastname: Mathies
mesh_terms: D004273:DNA, Neoplasm / Q000032:analysis*; D004276:DNA, Satellite / Q000032:analysis*; D019075:Electrophoresis, Capillary / Q000379:methods*; D004735:Energy Transfer; D005456:Fluorescent Dyes; D006801:Humans; D018895:Microsatellite Repeats; D012091:Repetitive Sequences, Nucleic Acid; D001749:Urinary Bladder Neoplasms / Q000175:diagnosis* / Q000235:genetics
chemical_list: D004273:DNA, Neoplasm; D004276:DNA, Satellite; D005456:Fluorescent Dyes