title: Detection via laser desorption and mass spectrometry of multiplex electrophore-labeled albumin.
abstract: Albumin was reacted with a mixture of six electrophore N-hydroxysuccinimide esters, each of which possessed an interior glycolketo linkage. The purpose of this linkage is to release the attached electrophore as a ketone when heated, due to a thermal retro-aldol reaction. The multiplex electrophore-labeled albumin was detected as a dried spot deposited on a polyimide membrane by laser desorption/capillary collection (of the released ketone electrophores)/off-line gas chromatography/electron capture-mass spectrometry. This encourages further study of such electrophore labels in immunoassays and related techniques, where there is a need to make advances in multi-analyte detection.
authors.affiliation: Department of Pharmaceutical Sciences, Bouvé College of Pharmacy and Health Professions, Barnett Institute, Northeastern University, Boston, MA 02115, USA.
authors.forename: N
authors.initials: N
authors.lastname: Bian
authors.forename: P
authors.initials: P
authors.lastname: Wang
authors.forename: Z
authors.initials: Z
authors.lastname: Wang
authors.forename: L
authors.initials: L
authors.lastname: Xu
authors.forename: G
authors.initials: G
authors.lastname: Church
authors.forename: R W
authors.initials: RW
authors.lastname: Giese
mesh_terms: D000418:Albumins / Q000032:analysis*; D008401:Gas Chromatography-Mass Spectrometry; D007834:Lasers; D013058:Mass Spectrometry; D012997:Solvents
chemical_list: D000418:Albumins; D012997:Solvents