Noninfectious virus-like particles produced by Moloney murine leukemia virus-based retrovirus packaging cells deficient in viral envelope become infectious in the presence of lipofection reagents.
Retrovirus packaging cell lines expressing the Moloney murine leukemia virus gag and pol genes but lacking virus envelope genes produce virus-like particles constitutively, whether or not they express a transcript from an integrated retroviral provirus. In the absence of a proviral transcript, the assembled particles contain processed gag and reverse transcriptase, and particles made by cells expressing an integrated lacZ provirus also contain viral RNA. The virus-like particles from both cell types are enveloped and are secreted/budded into the extracellular space but are noninfectious. Their physicochemical properties are similar to those of mature retroviral particles. The noninfectious gag pol RNA particles can readily be made infectious by the addition of lipofection reagents to produce preparations with titers of up to 10(5) colony-forming units per ml.
Sharma
S
S
Department of Pediatrics, Center for Molecular Genetics, University of California at San Diego, La Jolla, California 92093-0634, USA.
Murai
F
F
Miyanohara
A
A
Friedmann
T
T
D002460:Cell Line; D017077:Culture Media, Conditioned; D015750:Genes, gag; D015751:Genes, pol; D007202:Indicators and Reagents; D008854:Microscopy, Electron; D008979:Moloney murine leukemia virus / Q000472:pathogenicity / Q000502:physiology* / Q000648:ultrastructure; D012367:RNA, Viral; D014162:Transfection / Q000379:methods*; D014771:Virion / Q000472:pathogenicity / Q000502:physiology* / Q000648:ultrastructure; D019065:Virus Assembly
D017077:Culture Media, Conditioned; D007202:Indicators and Reagents; D012367:RNA, Viral