title: Comparative studies on tetrachloroethene reductive dechlorination mediated by Desulfitobacterium sp. strain PCE-S.
abstract: Tetrachloroethene reductive dechlorination was studied with cell extracts of a newly isolated, tetrachloroethene-utilizing bacterium, Desulfitobacterium sp. strain PCE-S. Tetrachloroethene dehalogenase mediated the reductive dechlorination of tetrachloroethene and trichloroethene to cis-1,2-dichloroethene with artificial electron donors such as methyl viologen. The chlorinated aromatic compounds tested so far were not reduced. A low-potential electron donor (E0' < -0.4 V) was required for tetrachloroethene reduction. The enzyme in its reduced state was inactivated by propyl iodide and reactivated by light, indicating the involvement of a corrinoid in reductive tetrachloroethene dechlorination.
authors.affiliation: Institut für Mikrobiologie, Universität Stuttgart, Germany.
authors.forename: E
authors.initials: E
authors.lastname: Miller
authors.forename: G
authors.initials: G
authors.lastname: Wohlfarth
authors.forename: G
authors.initials: G
authors.lastname: Diekert
mesh_terms: D001421:Bacteria, Anaerobic / Q000187:drug effects / Q000201:enzymology* / Q000378:metabolism*; D001673:Biodegradation, Environmental; D002614:Chelating Agents / Q000494:pharmacology; D004396:Coloring Agents / Q000494:pharmacology; D004734:Energy Metabolism / Q000187:drug effects; D004789:Enzyme Activation / Q000187:drug effects; D004791:Enzyme Inhibitors / Q000494:pharmacology; D006867:Hydrolases / Q000037:antagonists & inhibitors / Q000378:metabolism; D007211:Indoles / Q000494:pharmacology; D010084:Oxidation-Reduction; D013750:Tetrachloroethylene / Q000378:metabolism*
chemical_list: D002614:Chelating Agents; D004396:Coloring Agents; D004791:Enzyme Inhibitors; D007211:Indoles; C049784:n-propyl Astra blue; D006867:Hydrolases; C078610:2,3,5,6-tetrachloro-4-hydroquinone reductive dehalogenase; D013750:Tetrachloroethylene