Phenotype expression of human bone cells cultured on implant substrates.
Bone cells derived from the human jaw were cultured on titanium, titanium coated with hydroxyapatite (THA) or with plasma spray (TPS) to study the behaviour of the cells anchored to implant substrates. Bone cells were cultured in MEM with the addition of [3H]-thymidine to evaluate cellular proliferation, and [3H]-glucosamine to evaluate GAG synthesis and accumulation in the extra-cellular matrix (ECM). Moreover, to study the degradation of GAG bone cells were cultured in the presence of NH4Cl, an amine known to inhibit lysosomal activity. Our results show that TPS is the substrate that favours both cellular proliferation and the accumulation of GAG in the ECM.
Locci
P
P
Department of Experimental Medicine, University of Perugia, Italy.
Becchetti
E
E
Pugliese
M
M
Rossi
L
L
Belcastro
S
S
Calvitti
M
M
Pietrarelli
G
G
Staffolani
N
N
D000643:Ammonium Chloride; D001842:Bone and Bones / Q000166:cytology* / Q000378:metabolism / Q000648:ultrastructure; D002455:Cell Division; D002478:Cells, Cultured; D017886:Durapatite; D005109:Extracellular Matrix / Q000378:metabolism; D005944:Glucosamine / Q000378:metabolism / Q000494:pharmacology; D006025:Glycosaminoglycans / Q000096:biosynthesis / Q000235:genetics; D006801:Humans; D008247:Lysosomes / Q000187:drug effects / Q000378:metabolism; D008422:Materials Testing; D008855:Microscopy, Electron, Scanning; D010006:Osteoblasts / Q000166:cytology / Q000378:metabolism / Q000648:ultrastructure; D010641:Phenotype; D013936:Thymidine / Q000378:metabolism / Q000494:pharmacology; D014025:Titanium; D014316:Tritium
D006025:Glycosaminoglycans; D000643:Ammonium Chloride; D014316:Tritium; D017886:Durapatite; D014025:Titanium; D005944:Glucosamine; D013936:Thymidine