title: A bioactive 60-kilodalton prolactin species is preferentially secreted in cultures of mitogen-stimulated and nonstimulated peripheral blood mononuclear cells from subjects with systemic lupus erythematosus.
abstract: We have evaluated the production of PRL by human peripheral mononuclear cells (PBMNC) from normal subjects and patients with systemic lupus erythematosus (SLE). Conditioned medium prepared from basal and Con-A-stimulated PBMNC was assessed for the presence of PRL-like by its ability to stimulate growth of PRL-responsive Nb2 rat lymphoma cells. In the presence or absence of Con-A, SLE PBMNC secrete significantly higher (P < 0.001) amounts of bioactive PRL-like species than normal cells. Growth of Nb2 cells by conditioned medium was inhibited with specific antiserum to human PRL. Western blotting using a polyclonal antibody to human PRL revealed a single 60-kDa PRL-like species in both normal and SLE PBMNC extracts, the immunoreactivity of which was preferentially found in SLE subjects. With the use of reverse transcription-PCR an expected 633-bp band was observed, and its similarity to pituitary PRL was further confirmed by Southern blot analysis with human PRL complementary DNA as a probe. We conclude that a high molecular mass PRL-like species is synthesized and secreted by PBMNC, and patients with SLE have an increased secretion of lymphocyte-derived PRL-like material.
authors.affiliation: Department of Reproductive Biology, Instituto Nacional de la Nutrición Salvador Zubirán, Mexico City, Mexico.
authors.forename: F
authors.initials: F
authors.lastname: Larrea
authors.forename: A
authors.initials: A
authors.lastname: Martínez-Castillo
authors.forename: V
authors.initials: V
authors.lastname: Cabrera
authors.forename: J
authors.initials: J
authors.lastname: Alcocer-Varela
authors.forename: G
authors.initials: G
authors.lastname: Queipo
authors.forename: C
authors.initials: C
authors.lastname: Cariño
authors.forename: D
authors.initials: D
authors.lastname: Alarcón-Segovia
mesh_terms: D000293:Adolescent; D000328:Adult; D000818:Animals; D001681:Biological Assay; D015139:Blotting, Southern; D015153:Blotting, Western; D002478:Cells, Cultured; D003208:Concanavalin A / Q000494:pharmacology; D017077:Culture Media, Conditioned; D005260:Female; D015870:Gene Expression; D006801:Humans; D007963:Leukocytes, Mononuclear / Q000378:metabolism*; D008180:Lupus Erythematosus, Systemic / Q000097:blood*; D008223:Lymphoma / Q000473:pathology; D008970:Molecular Weight; D016133:Polymerase Chain Reaction; D011388:Prolactin / Q000235:genetics / Q000378:metabolism* / Q000494:pharmacology; D012333:RNA, Messenger / Q000032:analysis; D051381:Rats
chemical_list: D017077:Culture Media, Conditioned; D012333:RNA, Messenger; D003208:Concanavalin A; D011388:Prolactin