[Enhanced metastatic potential of rat RG2 glioblastoma cells transfected with CD44v (meta-1) sequence].
It is extremely rare for brain glioblastoma to metastasize extracranially. To elucidate whether glioblastoma cells remain non-metastatic when ectopically transplanted and CD44v gene is introduced, glioblastoma RG2-m cells were transfected with CD44v gene sequence encompassing v3-v10. After selection in culture medium with G418, CD44v expressing RG2-a cells were cloned. With RG2-a and its parental counterpart RG2-m, a series of experimental metastasis assays were performed in syngeneic F344 rats. When inoculated subcutaneously and in the foot-pad, both RG2-m and RG2-a cells metastasized spontaneously to the regional lymph nodes and lungs. However, metastasis of RG2-a was more severe and more organs were involved when compared to that of the parental RG2-m cells. The results indicate that glioblastoma becomes metastatic if extracranially transplanted and expression of CD44v would further enhance its metastatic patential.
Li
H
H
Division of Molecular Bioligy, Shenyang Medical College.
Hofmann
M
M
Liu
J
J
D000818:Animals; D001932:Brain Neoplasms / Q000473:pathology*; D005909:Glioblastoma / Q000556:secondary*; D018960:Hyaluronan Receptors / Q000235:genetics*; D009368:Neoplasm Transplantation; D010957:Plasmids; D051381:Rats; D011916:Rats, Inbred F344; D014162:Transfection; D014407:Tumor Cells, Cultured
D018960:Hyaluronan Receptors