Anti-idiotype RNA selected with an anti-nuclear export signal antibody is actively transported in oocytes and inhibits Rev- and cap-dependent RNA export.
The anti-idiotype approach is based on the assumption that an antibody specific for a receptor-binding domain of a ligand could be structurally related to the receptor. Therefore, a structural mimic of a receptor-binding domain, selected with an anti-ligand antibody, might be a functional substrate for the receptor. This hypothesis was addressed here by generating antibodies recognizing the Rev-nuclear export signal (NES). A functional NES is required for active export, presumably by interacting directly or indirectly with the nuclear pore complex. Anti-NES antibodies were used to isolate RNA mimics of the NES peptide from combinatorial RNA libraries. The RNA-mimics are exported actively, block Rev-dependent export of a reporter RNA, and inhibit cap-dependent U1 snRNA export in Xenopus oocytes, properties previously reported for NES-peptide conjugates.
Hamm
J
J
Department of Biochemistry, University of Dundee, United Kingdom.
Huber
J
J
Lührmann
R
R
D000818:Animals; D000888:Antibodies, Anti-Idiotypic / Q000276:immunology*; D001666:Binding Sites, Antibody; D001692:Biological Transport; D002467:Cell Nucleus / Q000378:metabolism*; D015694:Gene Products, rev / Q000037:antagonists & inhibitors* / Q000378:metabolism*; D009690:Nucleic Acid Conformation; D009865:Oocytes / Q000378:metabolism*; D012313:RNA / Q000737:chemistry / Q000276:immunology / Q000378:metabolism*; D012315:RNA Caps; D013696:Temperature; D014158:Transcription, Genetic; D014981:Xenopus
D000888:Antibodies, Anti-Idiotypic; D015694:Gene Products, rev; D012315:RNA Caps; D012313:RNA