pp1-cyano2, a protein serine/threonine phosphatase 1 gene from the cyanobacterium Microcystis aeruginosa UTEX 2063.
Polymerase chain reaction (PCR) products similar to protein serine/threonine family I phosphatase genes were identified in five strains of cyanobacteria from three species. The gene for one of these protein phosphatase PCR products, pp1-cyano2 from Microcystis aeruginosa UTEX 2063, was cloned and sequenced. The deduced protein sequence PP1-cyano2 contains 264 amino acid residues ( approximately 30.3 kDa). In its N-terminal region, PP1-cyano2 had a GDXXHG(X)nGDXXDRG(X)nGNHE (nP23) sequence that is well-conserved in all protein serine/threonine family I phosphatases. Of 19 amino acid residues important for either metal binding, structure of the active site, or catalysis in eukaryotic PP1, 18 were present in PP1-cyano2. Reverse-transcription-PCR results showed that pp1-cyano2 was expressed under laboratory culture conditions.
Shi
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The Biomedical Sciences PhD Program, Wright State University, Dayton, OH 45435, USA.
Carmichael
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D000595:Amino Acid Sequence; D001665:Binding Sites; D002384:Catalysis; D008670:Metals / Q000378:metabolism; D046931:Microcystis / Q000201:enzymology* / Q000235:genetics*; D008969:Molecular Sequence Data; D005810:Multigene Family; D010749:Phosphoprotein Phosphatases / Q000737:chemistry / Q000235:genetics* / Q000302:isolation & purification; D016133:Polymerase Chain Reaction; D054645:Protein Phosphatase 1
D008670:Metals; D010749:Phosphoprotein Phosphatases; D054645:Protein Phosphatase 1