Temporal and spatial specificity of PDGF alpha receptor promoter in transgenic mice.
Aberrant expression of the platelet-derived growth factor alpha receptor (PDGF alpha R) has been linked to developmental abnormalities in vertebrate models, and has been implicated in multiple disease states in humans. To identify cis-acting regulatory elements that dictate expression of this receptor, we generated transgenic mice bearing the reporter gene beta-galactosidase (lacZ) under the control of a 6-kb promoter sequence. Expression of lacZ was monitored throughout embryonic development, with special focus on nervous tissue, skeleton, and several organ systems wherein PDGF alpha R expression is thought to play a pivotal role. In several independent transgenic mouse strains, lacZ expression recapitulated predominant features of PDGF alpha R gene expression during mouse development. These results demonstrate that critical tissue-specific regulatory elements for PDGF alpha R expression are located within a 6-kb upstream region of the PDGF alpha R gene.
Reinertsen
K K
KK
Department of Microbiology and Molecular Genetics, Harvard Medical School, Boston, MA, USA.
Bronson
R T
RT
Stiles
C D
CD
Wang
C
C
D000818:Animals; D001842:Bone and Bones / Q000196:embryology / Q000378:metabolism; D005314:Embryonic and Fetal Development; D005775:Gastrula; D015870:Gene Expression; D018507:Gene Expression Regulation, Developmental; D007763:Lac Operon; D051379:Mice; D008822:Mice, Transgenic; D009420:Nervous System / Q000196:embryology / Q000378:metabolism; D011401:Promoter Regions, Genetic; D017479:Receptors, Platelet-Derived Growth Factor / Q000096:biosynthesis / Q000235:genetics* / Q000378:metabolism; D011993:Recombinant Fusion Proteins
D011993:Recombinant Fusion Proteins; D017479:Receptors, Platelet-Derived Growth Factor