Increased expression of PDGF and c-myc genes in lungs and pulmonary arteries of pulmonary hypertensive rats induced by hypoxia.
The role of growth factors and proto-oncogene in pulmonary vascular structural remodelling is not well known. The present study examined gene expression of platelet-derived growth factor (PDGF)-A and -B chain and proto-oncogene, c-myc, in lung tissue and pulmonary artery of rats exposed to hypoxia and compared to those levels of gene expression in normal rats. Normal lungs and pulmonary artery expressed PDGF-A chain transcript of 1.7 kb and PDGF-B chain transcript of 3.5 Kb. The c-myc transcript of 2.2 kb was expressed as well. After hypoxic exposure for 7 and 14 days mRNA levels of PDGF-B chain and c-myc were elevated significantly compared with those of control rats. PDGF-A chain mRNA increased after hypoxia for 7 days, and then declined. These results suggest that activation of autocrine and/or paracrine is important in proliferation mechanism of pulmonary artery smooth muscle cells in hypoxic pulmonary hypertensive rats.
Cai
Y
Y
Department of Physiology, Institute of Basic Medical Sciences, CAMS, Beijing.
Han
M
M
Luo
L
L
Song
W
W
Zhou
X
X
D000818:Animals; D004285:Dogs; D015870:Gene Expression; D016259:Genes, myc; D006976:Hypertension, Pulmonary / Q000235:genetics* / Q000378:metabolism; D000860:Hypoxia / Q000150:complications*; D008168:Lung / Q000378:metabolism; D008297:Male; D010982:Platelet-Derived Growth Factor / Q000096:biosynthesis*; D011651:Pulmonary Artery / Q000378:metabolism; D012333:RNA, Messenger / Q000096:biosynthesis; D051381:Rats; D017208:Rats, Wistar
D010982:Platelet-Derived Growth Factor; D012333:RNA, Messenger; C095477:platelet-derived growth factor AB