Protective effects of vitamin E and C on cisplatin nephrotoxicity in developing rats.
The kinetics of vitamin E was followed in serum, liver and kidney of 10- and 55-day-old rats after the administration of a single i.m. dose of 100 mg alpha-tocopherol acetate/100 g body wt. The basal levels without vitamin E administration were significantly higher in serum and liver of 10- than 55-day-old rats. The effect of vitamin E on cisplatin (CP; 0.6 mg/100 g body wt., i.p.) nephrotoxicity was investigated by determining urinary volume and protein excretion, as well as the concentration of blood urea nitrogen (BUN) and lipid peroxides in renal tissue (LPO). Previously described age differences in CP nephrotoxicity were confirmed. The administration of vitamin E, 12 h prior to CP, diminished the toxic effect of CP in young and adult rats. This effect could not be enhanced by a second administration of vitamin E. The simultaneous administration of vitamin E and C 12 h prior to CP intensified the protective effect of a single administration of vitamin E in 10- and 55-day-old rats without influencing the concentration of platinum in renal tissue.
Appenroth
D
D
Institute of Pharmacology and Toxicology, Friedrich Schiller University, Jena, Germany.
Fröb
S
S
Kersten
L
L
Splinter
F K
FK
Winnefeld
K
K
D000375:Aging / Q000502:physiology; D000818:Animals; D000970:Antineoplastic Agents / Q000037:antagonists & inhibitors* / Q000493:pharmacokinetics / Q000633:toxicity*; D001205:Ascorbic Acid / Q000493:pharmacokinetics / Q000494:pharmacology*; D001806:Blood Urea Nitrogen; D002945:Cisplatin / Q000037:antagonists & inhibitors* / Q000493:pharmacokinetics / Q000633:toxicity*; D005260:Female; D007674:Kidney Diseases / Q000139:chemically induced / Q000517:prevention & control*; D008054:Lipid Peroxides / Q000097:blood; D008297:Male; D010984:Platinum / Q000493:pharmacokinetics; D051381:Rats; D017208:Rats, Wistar; D014810:Vitamin E / Q000493:pharmacokinetics / Q000494:pharmacology*
D000970:Antineoplastic Agents; D008054:Lipid Peroxides; D014810:Vitamin E; D010984:Platinum; D001205:Ascorbic Acid; D002945:Cisplatin