Viral mimicry: common mode of association with HCF by VP16 and the cellular protein LZIP.
Upon infection of human cells, the herpes simplex virus protein VP16 associates with the endogenous cell-proliferation factor HCF. VP16 can also associate with HCFs from invertebrates, suggesting that VP16 mimics a cellular protein whose interaction with HCF has been conserved. Here, we show that VP16 mimics the human basic leucine-zipper protein LZIP, which, through association with HCF, may control cell-cycle progression. VP16 and LZIP share a tetrapeptide motif-D/EHXY-used to associate with human HCF. The LZIP-related Drosophila protein BBF-2/dCREB-A contains this HCF-binding motif, indicating that the LZIP-HCF interaction has been conserved during metazoan evolution.
Freiman
R N
RN
Cold Spring Harbor Laboratory, Cold Spring Harbor, New York 11724, USA.
Herr
W
W
D000595:Amino Acid Sequence; D000818:Animals; D001665:Binding Sites; D002455:Cell Division; D002461:Cell Line, Transformed; D051699:Cyclic AMP Response Element-Binding Protein A; D004268:DNA-Binding Proteins / Q000378:metabolism; D029721:Drosophila Proteins; D004331:Drosophila melanogaster; D006367:HeLa Cells; D017405:Herpes Simplex Virus Protein Vmw65 / Q000378:metabolism*; D018259:Herpesvirus 1, Human / Q000378:metabolism*; D051863:Host Cell Factor C1; D006801:Humans; D016350:Leucine Zippers; D051379:Mice; D018716:Molecular Mimicry; D008969:Molecular Sequence Data; D011506:Proteins / Q000235:genetics / Q000378:metabolism*; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000378:metabolism; D017386:Sequence Homology, Amino Acid; D014157:Transcription Factors
C073555:CrebA protein, Drosophila; D051699:Cyclic AMP Response Element-Binding Protein A; D004268:DNA-Binding Proteins; D029721:Drosophila Proteins; C497692:HCFC1 protein, human; C497693:Hcfc1 protein, mouse; D017405:Herpes Simplex Virus Protein Vmw65; D051863:Host Cell Factor C1; D011506:Proteins; D011993:Recombinant Fusion Proteins; D014157:Transcription Factors