[Immunohistochemical study of lactate dehydrogenase isoenzymes in gastric cancer: light and electron microscopic observations].
In order to study the metabolic features of gastric cancer, the relationship between intestinal metaplasia or dysplasia and gastric cancer, and the relationship between LDH isoenzymes and biological behavior of gastric cancer, the content and distribution of lactate dehydrogenase (LDH) isoenzymes in 60 cases of gastric cancer were observed with immunohistochemical and immunoelectron microscopic techniques. The results showed that the content of LDH5 in gastric cancer cells was higher than that in any cells of normal gastric epithelium except parietal cells (P < 0.001). LDH-H (LDH1-LDH4) in cancer cells was lower than in parietal cells, but was not significantly different from that in chief cells and surface epithelium. Under electron microscope, LDH5 in cancer cells was mainly distributed in the matrix of cytoplasm, while LDH-H was scarce. LDH5 in the epithelium of intestinal metaplasia (IM) or dysplasia was higher than in pyloric glandular epithelium (P < 0.05), but was not significantly different from that in cancer cells (P > 0.2). LDH-H in the epithelium of IM or dysplasia was higher than that in pyloric glandular epithelium and gastric cancer cells (P < 0.05). The results suggest that increased LDH in gastric cancer cells is resulted mainly from increased LDH5, which makes the gastric cancer cells produce more lactate, with decrease in local pH. Invasion and spread of cancer cells could be indirectly promoted through an elevated activity of acid hydrolases. IM and dysplasia may be considered as borderline lesions from normal gastric mucosa to gastric cancer.
Song
Y
Y
Department of Pathology, Anhui Medical University, Hefei.
Yang
G
G
Dong
Y
Y
D005753:Gastric Mucosa / Q000201:enzymology / Q000473:pathology; D006801:Humans; D007150:Immunohistochemistry; D007527:Isoenzymes; D007770:L-Lactate Dehydrogenase / Q000378:metabolism*; D008679:Metaplasia / Q000201:enzymology; D011230:Precancerous Conditions / Q000201:enzymology / Q000473:pathology; D013274:Stomach Neoplasms / Q000201:enzymology* / Q000473:pathology
D007527:Isoenzymes; D007770:L-Lactate Dehydrogenase