title: Activity of 72-kDa and 92-kDa matrix metalloproteinases in placental tissues of cows with and without retained fetal membranes.
abstract: This study compared the activity of matrix metalloproteinases (MMP-2 and MMP-9) in retained and non-retained bovine placenta. The activities of MMPs and their zymogens were measured in fetal and maternal placental tissues from control cows (group B) and animals affected with retention of fetal membranes (group A) using a zymography technique on 10 per cent SDS polyacrylamide gels. The activity of proMMP-9 detected only in the maternal part of the placenta was lower in group A than in group B. ProMMP-2 activity was higher in group A than in group B in both tissues. The active forms of MMP-2 were observed in the maternal and fetal part of placenta in group B, but only the 68-kDa form was detected in the placental tissues of group A. The differences in enzyme activity between the groups and the lack of 64- and 60-kDa active forms of MMP-2 in the maternal and fetal parts of the retained placenta may have influenced the hydrolysis of collagen and the proper release of fetal membranes.
authors.affiliation: Department of Clinical Biochemistry and Environmental Toxicology, School of Medicine, Jaczewskiego, Poland.
authors.forename: J G
authors.initials: JG
authors.lastname: Maj
authors.forename: M
authors.initials: M
authors.lastname: Kankofer
mesh_terms: D000818:Animals; D002417:Cattle; D002418:Cattle Diseases / Q000201:enzymology*; D017364:Collagenases / Q000378:metabolism*; D003656:Decidua / Q000201:enzymology*; D004591:Electrophoresis, Polyacrylamide Gel; D005260:Female; D018093:Gelatinases / Q000378:metabolism*; D020778:Matrix Metalloproteinase 2; D020780:Matrix Metalloproteinase 9; D008666:Metalloendopeptidases / Q000378:metabolism*; D007744:Obstetric Labor Complications / Q000201:enzymology / Q000662:veterinary; D010920:Placenta; D018457:Placenta, Retained / Q000201:enzymology / Q000662:veterinary*; D011247:Pregnancy; D014327:Trophoblasts / Q000201:enzymology*
chemical_list: D017364:Collagenases; D018093:Gelatinases; D008666:Metalloendopeptidases; D020778:Matrix Metalloproteinase 2; D020780:Matrix Metalloproteinase 9