Inhibitory effect of 764-3 on Ca2+ uptake in rat brain synaptosomes.
In an attempt to examine the effect of 764-3, a purified component extracted from Radix Salviae Miltiorrhizae (RSM), on calcium channel, Ca2+ uptake in rat brain synaptosome stimulated by high-K+ and Glutamate (Glu) was investigated by using radioisotope labeled 45CaCl2. It was found that 764-3 could decrease the uptake of Ca2+ induced by high-K+ (60 mM) and 0.1 mM glutamate in a concentration-dependent manner. The maximum inhibiting rates were 45.22 +/- 4.88% and 37.58 +/- 4.52%, respectively. The results indicated that neuroprotective effect of RSM on cerebral ischemic injury may be related to its blocking effects on calcium channel.
Tao
Y
Y
Neurotransmitter Research Laboratory, Chinese PLA General Hospital, Postgraduate Military Medical School, Beijing, China.
Kuang
P
P
Zuo
P
P
D000818:Animals; D002118:Calcium / Q000378:metabolism*; D002121:Calcium Channel Blockers / Q000494:pharmacology*; D015220:Calcium Channels / Q000187:drug effects* / Q000378:metabolism; D002540:Cerebral Cortex / Q000378:metabolism*; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D008297:Male; D051381:Rats; D017208:Rats, Wistar; D013574:Synaptosomes / Q000378:metabolism*
D002121:Calcium Channel Blockers; D015220:Calcium Channels; D004365:Drugs, Chinese Herbal; C079053:IH 764-3; D002118:Calcium