Lymphocyte-conditioned medium in combination with interleukin-2 effectively induces antitumour autoimmunity by adoptive transfer of short activated killer (SHAK) cells.
In this study, effective antitumour immunity was transferred by autologous short activated killer (SHAK) cells induced over four hours with lymphocyte conditioned medium (LCM) and recombinant interleukin-2 (rIL-2). Among eight patients with progressive metastatic renal cell carcinoma refractory to standard therapy, there were six objective tumour responses to SHAKs. Progression-free survival ranged from 0 to 8+ months, and overall survival ranged from 2 to 14+ months, with a median of 9+ months. Systemic toxicity of SHAKs was limited to flulike symptoms. Patient SHAKs provided a tumour-specific immunity, both cellular and humoral (expression and secretion of secondary cytokines, including IL-2, GM-CSF, INF-gamma and TNF-alpha), far superior to rIL-2 activated killer cells.
Buer
J
J
Division of Haematology and Oncology, MHH University Medical Center, Hannover, Germany.
Hilse
R
R
Dallmann
I
I
Grosse
J
J
Kirchner
H
H
Zorn
U
U
Hänninen
E L
EL
Franzke
A
A
Duensing
S
S
Poliwoda
H
H
D019264:Adoptive Transfer / Q000009:adverse effects; D002292:Carcinoma, Renal Cell / Q000276:immunology / Q000401:mortality / Q000628:therapy*; D002478:Cells, Cultured; D017077:Culture Media, Conditioned; D016207:Cytokines / Q000096:biosynthesis*; D003602:Cytotoxicity, Immunologic; D018572:Disease-Free Survival; D006801:Humans; D007376:Interleukin-2 / Q000627:therapeutic use*; D007680:Kidney Neoplasms / Q000276:immunology / Q000401:mortality / Q000628:therapy*; D007694:Killer Cells, Natural / Q000637:transplantation*; D017710:Lymphocyte Transfusion; D008214:Lymphocytes / Q000166:cytology / Q000276:immunology*; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000096:biosynthesis; D011994:Recombinant Proteins / Q000627:therapeutic use; D015996:Survival Rate; D014158:Transcription, Genetic; D014182:Transplantation, Autologous
D017077:Culture Media, Conditioned; D016207:Cytokines; D007376:Interleukin-2; D012333:RNA, Messenger; D011994:Recombinant Proteins