A synthetic peptide administered with IL-12 elicits immunity to Listeria monocytogenes.
IL-12 is a pivotal cytokine signal for the development of Th1-type cellular responses that are required for control of intracellular pathogens. We previously demonstrated that coinjection of IL-12 with heat-killed Listeria monocytogenes, which was not immunogenic when injected alone, elicited intense Ag-specific T cell responses that conferred protection against subsequent challenge with Listeria. Herein we describe the remarkable finding that a nonimmunogenic synthetic peptide corresponding to a dominant MHC class II (H-2k)-restricted listerial determinant, when coinjected i.p. with murine IL-12, elicited potent Ag-specific immune responses that conferred protective immunity against Listeria.
Miller
M A
MA
Department of Microbiology and Immunology, Emory University School of Medicine, Atlanta, GA 30322, USA.
Skeen
M J
MJ
Ziegler
H K
HK
D000595:Amino Acid Sequence; D000818:Animals; D001427:Bacterial Toxins; D004338:Drug Combinations; D005260:Female; D006360:Heat-Shock Proteins / Q000008:administration & dosage / Q000138:chemical synthesis / Q000276:immunology*; D006460:Hemolysin Proteins; D007111:Immunity, Cellular; D007274:Injections, Intraperitoneal; D018664:Interleukin-12 / Q000008:administration & dosage / Q000276:immunology*; D008088:Listeriosis / Q000276:immunology* / Q000382:microbiology; D051379:Mice; D008809:Mice, Inbred C3H; D008969:Molecular Sequence Data; D010446:Peptide Fragments / Q000008:administration & dosage / Q000138:chemical synthesis / Q000276:immunology*; D013154:Spleen / Q000382:microbiology
D001427:Bacterial Toxins; D004338:Drug Combinations; D006360:Heat-Shock Proteins; D006460:Hemolysin Proteins; D010446:Peptide Fragments; D018664:Interleukin-12; C038705:hlyA protein, Listeria monocytogenes