title: Developmental fate of single embryonic stem cells microinjected into 8-cell-stage mouse embryos.
abstract: Embryonic stem (ES) cells are pluripotent and capable of differentiating into somatic as well as germ cell lineages when conjoined with blastomeres of early mouse embryos. However, the developmental potential of single ES cells has not been fully investigated. We injected single murine ES cells (A3-1 cell line) of 129 origin into 8-cell mouse embryos (B6xBDF1) and examined the patterns of distribution of ES-cell-derived cells in the blastocysts as well as in the fully grown chimeric mice. The ES cells underwent 1-2 cycles of mitosis between the 8-cell and the blastocyst stage when they were introduced as single cells, whereas those introduced as groups of 2-5 cells did not proliferate during the same period of development. The ES cells and their daughter cells were predominantly incorporated into the ICM. From the 63 8-cell embryos which received single ES cells microinjected into the perivitelline space, 24 newborns were obtained, and 4 (2 fertile males, 1 sterile female and 1 hermaphrodite) of them (16.6%) were chimeric. The test breeding studies revealed that all the progeny of the two chimeric males were derived from spermatozoa of 129 genotype. The relative contribution of the host-derived and the ES-cell-derived cells in different tissues of the chimeric mice was assessed by PCR analyses of the microsatellite polymorphism of genomic DNA extracted from the tissues. In two male germ line chimeras, the testes, the kidneys and the dorsal skeletal muscles exhibited exceptionally high 129 contents. Our results demonstrated that single ES cells which maintain totipotency or pluripotency of high degree are present in a colony of ES cells, and that single ES cells conjoined with the blastomeres of 8-cell-stage embryos may colonize, if the circumstances allow, almost exclusively the germ cells and concomitantly the urogenital cell lineages. Possible correlation between the allocation of the germ line and the urogenital lineages is discussed.
authors.affiliation: Department of Reproductive and Developmental Biology, University of Tokyo, Japan.
authors.forename: S
authors.initials: S
authors.lastname: Saburi
authors.forename: S
authors.initials: S
authors.lastname: Azuma
authors.forename: E
authors.initials: E
authors.lastname: Sato
authors.forename: Y
authors.initials: Y
authors.lastname: Toyoda
authors.forename: C
authors.initials: C
authors.lastname: Tachi
mesh_terms: D000818:Animals; D001755:Blastocyst; D002454:Cell Differentiation / Q000235:genetics*; D002678:Chimera; D004622:Embryo, Mammalian / Q000166:cytology* / Q000502:physiology*; D005260:Female; D005854:Germ Cells / Q000502:physiology; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D008815:Mice, Inbred Strains; D008845:Microinjections; D013234:Stem Cells / Q000502:physiology*