[Repression of voltage gated K+ channel expressed in Xenopus oocytes after injection total RNA from carp retina by an antisense oligonucleotide].
Injection of total RNA from carp retina induced the formation of a membrane K+ channel in Xenopus oocytes. We found an oligonucleotide complementary to the mRNA coding for a peptide fragment in the K+ channel of drosophila and mice could suppress the K+ channel current in a very specific manner. This provides important information for the cloning of this gene and for studying it's function.
Bao
Y D
YD
Laboratory of Retina Research, Shanghai Institute of Physiology, Chinese Academy of Sciences.
Zhu
H
H
Zhu
X
X
D000818:Animals; D002347:Carps; D015870:Gene Expression; D051379:Mice; D016376:Oligonucleotides, Antisense / Q000494:pharmacology*; D009865:Oocytes / Q000378:metabolism*; D015221:Potassium Channels / Q000096:biosynthesis*; D012313:RNA / Q000494:pharmacology; D012160:Retina; D014982:Xenopus laevis
D016376:Oligonucleotides, Antisense; D015221:Potassium Channels; D012313:RNA