title: In vitro isolation and characterisation of a bovine Neospora species in Japan.
abstract: Eleven aborted bovine fetuses and five calves suspected as having neosporosis were necropsied and tissues from these animals were inoculated into bovine cardiopulmonary aortic endothelial cells and monkey kidney cells and maintained at 37 degrees C with 5 per cent CO2. Neospora tachyzoites were observed in one cell 49 days after inoculation. The isolated parasite (JPA1) was morphologically identical to the previously reported bovine Neospora species (BPA1) and confirmed by its strong antigenic reactivity with bovine control antisera to Neospora species and its lack of reactivity with Toxoplasma gondii and Sarcocystis cruzi antisera. This is the first bovine Neospora species isolate in Asia and further studies with this isolate are now expected.
authors.affiliation: National Institute of Animal Health, Ibaraki, Japan.
authors.forename: I
authors.initials: I
authors.lastname: Yamane
authors.forename: T
authors.initials: T
authors.lastname: Kokuho
authors.forename: K
authors.initials: K
authors.lastname: Shimura
authors.forename: M
authors.initials: M
authors.lastname: Eto
authors.forename: T
authors.initials: T
authors.lastname: Shibahara
authors.forename: M
authors.initials: M
authors.lastname: Haritani
authors.forename: Y
authors.initials: Y
authors.lastname: Ouchi
authors.forename: K
authors.initials: K
authors.lastname: Sverlow
authors.forename: P A
authors.initials: PA
authors.lastname: Conrad
mesh_terms: D000034:Abortion, Veterinary; D000818:Animals; D000913:Antibodies, Protozoan / Q000032:analysis; D000953:Antigens, Protozoan / Q000032:analysis; D002417:Cattle; D002418:Cattle Diseases / Q000453:epidemiology / Q000469:parasitology*; D002460:Cell Line; D003048:Coccidiosis / Q000453:epidemiology / Q000469:parasitology / Q000662:veterinary*; D004730:Endothelium, Vascular / Q000166:cytology / Q000469:parasitology; D005260:Female; D005333:Fetus / Q000469:parasitology; D019084:Fluorescent Antibody Technique, Indirect / Q000662:veterinary; D000882:Haplorhini; D007564:Japan / Q000453:epidemiology; D007668:Kidney / Q000166:cytology / Q000469:parasitology; D008854:Microscopy, Electron / Q000379:methods / Q000662:veterinary; D018688:Neospora / Q000276:immunology / Q000302:isolation & purification* / Q000648:ultrastructure; D011247:Pregnancy; D012522:Sarcocystis / Q000276:immunology / Q000302:isolation & purification; D014122:Toxoplasma / Q000276:immunology / Q000302:isolation & purification
chemical_list: D000913:Antibodies, Protozoan; D000953:Antigens, Protozoan