Whole blood folate concentrations: comparison between Stratus Folate (DADE) and radioassay (DPC) methods.
The analytical performance of the Stratus Folate assay for intra-erythrocyte folate determination in normal subjects and in patients affected by folate-related diseases was compared with that of the radioassay (DPC) routinely employed by us. Folate concentrations were measured in freshly obtained EDTA whole blood from 100 subjects. Haemolysis was performed with the appropriate lysis reagent. In addition, to compare two different haemolysis procedures folate determination was carried out in 51 samples haemolysed according to the two procedures in parallel. Data were analyzed using Wilcoxon's test and standardized principal component analysis. Stratus Folate assay and radioassay performances were comparable in terms of analytical characteristics as well as in individual intraerythrocyte folate values across the range of whole blood concentrations examined in the survey. Significant differences were detected between the two different haemolysis procedures only for the radioassay. In conclusion, we observed no significant differences between the two folate determination methods despite their different analytical principles, which indicates the suitability of routine use of the automated non-isotopic Stratus Folate assay for clinical purposes. Moreover, with the latter assay the laboratory staff could choose the more convenient haemolysis procedure.
Bamonti-Catena
F
F
Istituto di Scienze Mediche, Università di Milano, Ospedale Maggiore-IRCCS, Italy. Filobus@IMIUCCA.CSI.UNIMI.IT
Porcella
A
A
Pomati
M
M
Franzini
C
C
Rosina
M
M
Cavalca
V
V
Maiolo
A T
AT
D001331:Automation; D004492:Edetic Acid; D004912:Erythrocytes / Q000378:metabolism*; D005215:Fasting / Q000097:blood; D005470:Fluorometry / Q000379:methods; D005492:Folic Acid / Q000097:blood*; D006461:Hemolysis; D006801:Humans; D011863:Radioimmunoassay / Q000379:methods
D005492:Folic Acid; D004492:Edetic Acid