Preliminary characterisation of an Onchocerca volvulus aspartic protease.
PCR using 1 primer specific for aspartic proteases and 1 primer annealing to the vector allowed amplification of a 377 bp fragment encoding part of an aspartic protease from an Onchocerca volvulus cDNA library. Use of this fragment as a probe allowed the isolation of a larger cDNA clone. In common with 2 other nematode aspartic proteases, the O. volvulus predicted protein has several of the general characteristics of this class of proteins, but lacks specific determinants of lysosomal localisation.
Jolodar
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Department of Biochemistry and Molecular Biology, James Cook University of North Queensland, Townsville, Australia.
Miller
D J
DJ
D000595:Amino Acid Sequence; D000818:Animals; D016282:Aspartic Acid Endopeptidases / Q000235:genetics*; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics; D008247:Lysosomes / Q000201:enzymology; D008969:Molecular Sequence Data; D017181:Onchocerca volvulus / Q000201:enzymology / Q000235:genetics*; D016133:Polymerase Chain Reaction; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid
D018076:DNA, Complementary; D016282:Aspartic Acid Endopeptidases