Isolation and characterization of an Azotobacter vinelandii algK mutant.
Random Tn5 mutagenesis over Azotobacter vinelandii mucoid strain ATCC 9046 produced strain LA21, a non-mucoid, non-encysting mutant, carrying the Tn5 insertion within a gene homologous to algK from Pseudomonas aeruginosa encoding a periplasmic protein. algK, algJ and algG were shown to be transcribed as part of the palg8-alg44-algK-algJ-algG operon. A non-polar algK mutant was constructed and showed a non-mucoid phenotype, indicating that algK is essential for alginate production.
Mejía-Ruíz
H
H
Departamento de Microbiología Molecular, Universidad Nacional Autónoma de México, Morelos, Mexico.
Moreno
S
S
Guzmán
J
J
Nájera
R
R
León
R
R
Soberón-Chávez
G
G
Espín
G
G
D000464:Alginates / Q000378:metabolism; D016948:Azotobacter vinelandii / Q000235:genetics* / Q000378:metabolism / Q000648:ultrastructure; D001426:Bacterial Proteins / Q000235:genetics*; D001483:Base Sequence; D002874:Chromosome Mapping; D003890:Desiccation; D005798:Genes, Bacterial; D020723:Glucuronic Acid; D006603:Hexuronic Acids; D008854:Microscopy, Electron; D005810:Multigene Family; D016254:Mutagenesis, Insertional; D009154:Mutation; D009876:Operon; D010641:Phenotype; D010957:Plasmids / Q000235:genetics; D011401:Promoter Regions, Genetic; D011550:Pseudomonas aeruginosa / Q000235:genetics; D014158:Transcription, Genetic
C104798:AlgK protein, Pseudomonas aeruginosa; D000464:Alginates; D001426:Bacterial Proteins; D006603:Hexuronic Acids; D020723:Glucuronic Acid