title: Effect of detergent treatment on the cross-linking of purified chromaffin granule membranes with F-actin.
abstract: Chromaffin granule-microfilament interaction has previously been proposed to play a potential role in the regulation of dopamine uptake into the granules as well as catecholamine secretion in the adrenal medullary cell. However, the microfilament-binding site on the granule surface has not yet been identified. To establish the conditions for solubilization of the binding site, the effects of different-type detergents on the cross-linking of chromaffin granule membranes with F-actin were examined. The cross-linking activity was abolished by treatment of the granule membranes with deoxycholate (DOC), but not Triton X-100 (TX100) and CHAPS. The addition of DOC-extract decreased the viscosity of F-actin solution, suggesting that solubilized proteins bind to F-actin, but not cause the cross-linking of actin filaments. These results indicate that the actin-binding site can be solubilized from chromaffin granule membranes by DOC treatment.
authors.affiliation: Department of Pharmacology, Tokushima University School of Medicine, Japan.
authors.forename: K
authors.initials: K
authors.lastname: Morita
authors.forename: H B
authors.initials: HB
authors.lastname: Pollard
mesh_terms: D000199:Actins / Q000187:drug effects* / Q000378:metabolism; D000313:Adrenal Medulla / Q000737:chemistry; D000818:Animals; D002417:Cattle; D002793:Cholic Acids / Q000494:pharmacology; D002837:Chromaffin Granules / Q000187:drug effects* / Q000378:metabolism; D003840:Deoxycholic Acid / Q000494:pharmacology; D003902:Detergents / Q000494:pharmacology*; D007425:Intracellular Membranes / Q000187:drug effects* / Q000378:metabolism; D008840:Microfilament Proteins / Q000187:drug effects / Q000378:metabolism; D017830:Octoxynol / Q000494:pharmacology; D014783:Viscosity / Q000187:drug effects
chemical_list: D000199:Actins; D002793:Cholic Acids; D003902:Detergents; D008840:Microfilament Proteins; D003840:Deoxycholic Acid; D017830:Octoxynol; C028213:3-((3-cholamidopropyl)dimethylammonium)-1-propanesulfonate