title: Sequence analysis of functional regions of homoserine dehydrogenase genes from L-lysine and L-threonine-producing mutants of Brevibacterium lactofermentum.
abstract: The homoserine dehydrogenase (HD) genes from Brevibacterium lactofermentum lysine- and threonine-producing mutants were cloned, using the polymerase chain reaction, and sequenced. We found the amino acid substitutions, Val104Ile in the lysine-producing mutants in which HD may cause leaky mutation and Ser393Phe in the threonine-producing mutant with feedback-insensitive HD.
authors.affiliation: Central Research Laboratories, Ajinomoto Co., Inc., Kawasaki, Japan.
authors.forename: M
authors.initials: M
authors.lastname: Sugimoto
authors.forename: A
authors.initials: A
authors.lastname: Tanaka
authors.forename: T
authors.initials: T
authors.lastname: Suzuki
authors.forename: H
authors.initials: H
authors.lastname: Matsui
authors.forename: S
authors.initials: S
authors.lastname: Nakamori
authors.forename: H
authors.initials: H
authors.lastname: Takagi
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D001951:Brevibacterium / Q000235:genetics* / Q000378:metabolism; D004269:DNA, Bacterial / Q000235:genetics; D005798:Genes, Bacterial / Q000235:genetics*; D006715:Homoserine Dehydrogenase / Q000737:chemistry* / Q000235:genetics; D008239:Lysine / Q000235:genetics / Q000378:metabolism; D008969:Molecular Sequence Data; D009154:Mutation / Q000235:genetics; D013912:Threonine / Q000235:genetics / Q000378:metabolism
chemical_list: D004269:DNA, Bacterial; D013912:Threonine; D006715:Homoserine Dehydrogenase; D008239:Lysine