title: Chicken thyroid hormone receptor alpha requires the N-terminal amino acids for exclusive nuclear localization.
abstract: The subcellular localization of natural and engineered forms of the chicken thyroid hormone receptor (cTR alpha) is dependent on amino acids encoded in the N-terminal region. The full length receptor protein, cTR alpha-p46, was found to localize exclusively to the nucleus, whereas the N-terminally shorter variant, cTR alpha-p40, localizes to both the nucleus and the cytoplasm. The exclusive nuclear localization of cTR alpha-p46 is dependent on the presence of the first 11 N-terminal amino acids, but independent of the phosphorylation of the serine at position 12. Our data identify a novel role for an N-terminal domain of the full length thyroid hormone receptor.
authors.affiliation: Department of Cell and Molecular Biology, Medical Nobel Institute, Karolinska Institute, Stockholm, Sweden.
authors.forename: M L
authors.initials: ML
authors.lastname: Andersson
authors.forename: B
authors.initials: B
authors.lastname: Vennström
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D002467:Cell Nucleus / Q000378:metabolism*; D002645:Chickens; D002822:Choriocarcinoma; D019992:Databases as Topic; D005260:Female; D006801:Humans; D008969:Molecular Sequence Data; D009865:Oocytes / Q000502:physiology; D017433:Protein Structure, Secondary; D011988:Receptors, Thyroid Hormone / Q000096:biosynthesis / Q000737:chemistry / Q000378:metabolism*; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000737:chemistry / Q000378:metabolism; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid; D014162:Transfection; D014407:Tumor Cells, Cultured; D014982:Xenopus laevis
chemical_list: D011988:Receptors, Thyroid Hormone; D011994:Recombinant Proteins