A nitric oxide-releasing reagent, S-nitroso-N-acetylpenicillamine, enhances the expression of superoxide dismutases mRNA in the murine macrophage cell line RAW264-7.
Murine interferon-gamma (IFN-gamma) stimulates the murine macrophage tumour cell line RAW264-7 to produce nitric oxide (NO). IFN-gamma induces expression of inducible NO synthase (iNOS), manganese superoxide dismutase (Mn-SOD) and copper zinc SOD (CuZn-SOD) in these cells. To investigate the mechanism of induction of SOD expression, we added S-nitroso-N-acetyl penicillamine (SNAP) to RAW264-7 cells. SNAP enhanced the expression of Mn-SOD and CuZn-SOD. These results suggest that when producing NO, RAW264-7 cells express SOD that might protect them from NO toxicity.
Sano
H
H
First Department of Internal Medicine, Nagoya University School of Medicine, Aichi, Japan.
Hirai
M
M
Saito
H
H
Nakashima
I
I
Isobe
K I
KI
D000818:Animals; D003300:Copper / Q000378:metabolism; D015870:Gene Expression; D007371:Interferon-gamma / Q000494:pharmacology; D008264:Macrophages / Q000187:drug effects / Q000201:enzymology* / Q000378:metabolism; D017895:Manganese Compounds / Q000378:metabolism; D051379:Mice; D009569:Nitric Oxide / Q000378:metabolism*; D010396:Penicillamine / Q000031:analogs & derivatives* / Q000494:pharmacology; D012333:RNA, Messenger / Q000235:genetics; D026423:S-Nitroso-N-Acetylpenicillamine; D013482:Superoxide Dismutase / Q000235:genetics / Q000378:metabolism*; D014407:Tumor Cells, Cultured; D014665:Vasodilator Agents / Q000494:pharmacology*; D017967:Zinc Compounds / Q000378:metabolism
D017895:Manganese Compounds; D012333:RNA, Messenger; D014665:Vasodilator Agents; D017967:Zinc Compounds; D009569:Nitric Oxide; D003300:Copper; D026423:S-Nitroso-N-Acetylpenicillamine; D007371:Interferon-gamma; D013482:Superoxide Dismutase; D010396:Penicillamine