title: Developmentally regulated markers in the postnatal cervical spinal cord of the opossum Monodelphis domestica.
abstract: The aim of this study was to identify developmentally regulated immunocytochemical markers to assess development in the cervical spinal cord of Monodelphis domestica. We demonstrate that two commercially available antibodies exhibit altered patterns of distribution during early postnatal development. Although neurofilament staining was present at birth, only the phosphorylated form, recognised by monoclonal antibodies 2F11 or SMI31 could be detected. Non-phosphorylated neurofilament, recognised by monoclonal antibody SMI32, only became detectable around postnatal day 4 (P4) but was restricted to cells in the ventral horn until 5 weeks postnatum. By 7.5 weeks, SMI32 immunoreactivity (IR) was found throughout the grey matter in a pattern similar to that in the adult for both SMI32 and microtubule-associated protein 2 (MAP2). The intermediate filament proteins, glial fibrillary acidic protein (GFAP) and vimentin (VIM), were detectable at birth in radially oriented, fibrous cells, but GFAP-IR was restricted to the ventral half of the cord. This ventral to dorsal gradient of GFAP-IR diminished during the first week of postnatal life, disappearing by P8. Many astrocyte-like, GFAP-positive cells were clearly present by 38 days and, in the adult, were abundant in the white matter. A few VIM-IR cells remained in the adult cord, also within the white matter. We suggest that SMI32 and GFAP are useful, developmentally regulated markers for studies of opossum spinal cord development. We are currently using these markers to investigate the pronounced rostral to caudal gradient in the postnatal spinal cord and to assess development in the cultured spinal cord.
authors.affiliation: Division of Neuroscience and Biomedical Systems, University of Glasgow, UK. l.breckenridge@bio.gla.ac.uk
authors.forename: L J
authors.initials: LJ
authors.lastname: Breckenridge
authors.forename: I U
authors.initials: IU
authors.lastname: Sommer
authors.forename: S E
authors.initials: SE
authors.lastname: Blackshaw
mesh_terms: D000375:Aging; D000818:Animals; D000831:Animals, Newborn; D000911:Antibodies, Monoclonal; D015415:Biomarkers; D005904:Glial Fibrillary Acidic Protein / Q000032:analysis; D007150:Immunohistochemistry; D008869:Microtubule-Associated Proteins / Q000032:analysis; D016900:Neurofilament Proteins / Q000032:analysis*; D009893:Opossums / Q000254:growth & development*; D010766:Phosphorylation; D013116:Spinal Cord / Q000166:cytology / Q000254:growth & development*; D014746:Vimentin / Q000032:analysis
chemical_list: D000911:Antibodies, Monoclonal; D015415:Biomarkers; D005904:Glial Fibrillary Acidic Protein; D008869:Microtubule-Associated Proteins; D016900:Neurofilament Proteins; D014746:Vimentin