title: Effect of dicetylphosphate or stearic acid on spontaneous transfer of protein from influenza virus-infected cells to dimyristoylphosphatidylcholine liposomes.
abstract: Membrane proteins, such as viral spike, were transferred spontaneously from influenza virus-infected cells to various liposomes. The protein transfer was enhanced by the presence of negative charged component dicetylphosphate (DCP) or stearic acid (SA) in dimyristoylphosphatidylcholine (DMPC) liposomes. The lowering of membrane fluidity did not relate to the effect of DCP or SA on protein transfer in this study. We considered that the alteration of membrane properties, such as construction of the surface or stability of transferred protein in liposomes, due to the specific structure of DCP or SA is responsible for the enhancement of spontaneous protein transfer by the presence of the amphiphilic components.
authors.affiliation: Faculty of Pharmaceutical Sciences, Toyama Medical and Pharmaceutical University, Japan.
authors.forename: K
authors.initials: K
authors.lastname: Kogure
authors.forename: C
authors.initials: C
authors.lastname: Nakamura
authors.forename: O
authors.initials: O
authors.lastname: Okuda
authors.forename: K
authors.initials: K
authors.lastname: Hayashi
authors.forename: M
authors.initials: M
authors.lastname: Ueno
mesh_terms: D000818:Animals; D002460:Cell Line; D004134:Dimyristoylphosphatidylcholine / Q000378:metabolism; D004161:Diphenylhexatriene / Q000378:metabolism; D004594:Electrophysiology; D005454:Fluorescence Polarization; D005456:Fluorescent Dyes / Q000378:metabolism; D005470:Fluorometry; D000882:Haplorhini; D007668:Kidney / Q000821:virology; D008081:Liposomes / Q000378:metabolism*; D008560:Membrane Fluidity / Q000187:drug effects; D010755:Organophosphates / Q000494:pharmacology*; D009975:Orthomyxoviridae / Q000378:metabolism*; D013229:Stearic Acids / Q000494:pharmacology*; D014763:Viral Matrix Proteins / Q000378:metabolism*
chemical_list: D005456:Fluorescent Dyes; D008081:Liposomes; D010755:Organophosphates; D013229:Stearic Acids; D014763:Viral Matrix Proteins; D004161:Diphenylhexatriene; C002290:dicetylphosphate; C031183:stearic acid; D004134:Dimyristoylphosphatidylcholine