Vasodilator effects on canine basilar artery induced by intracisternal interleukin-1 beta.
The effect of interleukin-1 beta (IL-1 beta) on a cerebral artery was investigated in anesthetized dogs. Intracisternal administration of IL-1 beta (0.03 and 0.3 micrograms) dilated the canine basilar artery in a dose-dependent manner, without affecting systemic blood pressure or heart rate. The increase in diameter induced by 0.3 micrograms of IL-1 beta was 28.4% +/- 13.4% of control at 2 hours and was inhibited by 30 micrograms of the IL-1 beta receptor antagonist, zinc protoporphyrin (4.5% +/- 13.5%, P < 0.05). Interleukin-1 beta did not affect the concentration of nitric oxide metabolites in CSF. However, there was an increase in the concentration of eicosanoids in CSF, and the elevation of 6-keto-PGF1 alpha paralleled the vasodilation. Pretreatment with 30 micrograms of the selective inducible cyclooxygenase (COX-2) inhibitor NS-398 also inhibited the IL-1 beta-induced vasodilation significantly (5.9% +/- 9.4% at 2 hours, P < 0.01). Western blot analysis revealed the expression of a 68-kD COX-2-like protein in basilar artery extracts. These findings suggest that the IL-1 beta-induced vasodilator effect is linked to the prostaglandin cascade, predominantly to prostaglandin I2, by induction of COX-2, but not to the stimulation of nitric oxide metabolism.
Osuka
K
K
Department of Neurosurgery, Nagoya University School of Medicine, Japan.
Suzuki
Y
Y
Watanabe
Y
Y
Dogan
A
A
Takayasu
M
M
Shibuya
M
M
Yoshida
J
J
D000818:Animals; D001488:Basilar Artery / Q000187:drug effects / Q000503:physiopathology*; D004285:Dogs; D005260:Female; D007276:Injections, Intraventricular; D007375:Interleukin-1 / Q000008:administration & dosage*; D008297:Male; D009569:Nitric Oxide / Q000502:physiology*; D014664:Vasodilation / Q000187:drug effects*
D007375:Interleukin-1; D009569:Nitric Oxide