Inhibitory effect of macrolides on interleukin-8 secretion from cultured human nasal epithelial cells.
The mechanism of macrolide therapy in chronic sinusitis patients is unclear. The authors studied the effect of macrolides on interleukin (IL)-8 secretion from cultured human nasal epithelial cells. Epithelial cells harvested from the nasal polyps of patients with chronic sinusitis were primary-cultured, and secreted IL-8 in culture media was measured by enzyme immunoassay. The cells secreted considerable amounts of IL-8 constitutively and in response to lipopolysaccharide. The secretion was significantly inhibited by 10(-5) M of erythromycin, clarithromycin, roxithromycin, and josamycin. 10(-6) M erythromycin still showed the inhibitory effect, whereas the same concentration of josamycin did not. These results indicate that macrolide antibiotics may act as an immunomodulator to reduce IL-8 in inflammatory sites and, at least partially, account for the clinically discrepant effects between 14- and 16-membered ring macrolides in long-term low-dose therapy for chronic sinusitis.
Suzuki
H
H
Department of Otolaryngology, Tohoku University School of Medicine, Sendai, Japan.
Shimomura
A
A
Ikeda
K
K
Furukawa
M
M
Oshima
T
T
Takasaka
T
T
D000328:Adult; D000368:Aged; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D018929:Cell Culture Techniques; D002908:Chronic Disease; D004305:Dose-Response Relationship, Drug; D004724:Endoscopy; D004847:Epithelial Cells / Q000187:drug effects*; D005260:Female; D006801:Humans; D007124:Immunoenzyme Techniques; D016209:Interleukin-8 / Q000378:metabolism*; D018942:Macrolides; D008297:Male; D008875:Middle Aged; D009297:Nasal Mucosa / Q000187:drug effects* / Q000378:metabolism*; D009298:Nasal Polyps / Q000150:complications / Q000601:surgery; D012852:Sinusitis / Q000150:complications / Q000188:drug therapy
D000900:Anti-Bacterial Agents; D016209:Interleukin-8; D018942:Macrolides