Detection of polyanion-restricted anti-histone antibodies in patients with systemic lupus erythematosus.
The nature of the antibodies responsible for lupus erythematosus (LE) cells in systemic lupus erythematosus (SLE) remains obscure. We examined whether polyanion-restricted anti-histone antibodies were present in serum of patients with SLE using Western blotting analysis. Dextran sulfate or alginate was used as a polyanion compound in place of DNA. Antibodies which recognized dextran sulfate-histone complexes were present in serum of patients with SLE (17/34, 50%). These antibodies were detected in most SLE patients positive for LE cells (17/18, 94%) but not in those negative for LE cells or in patients with other collagen diseases. Similar results were obtained using alginate-histone complexes as antigens for Western blotting analysis. The antibodies to dextran sulfate-histone or alginate-histone complexes in serum of SLE patients were completely absorbed by treatment of serum with DNA-histone complexes, while they were unaffected by treatment with DNA only. The presence of antibodies to free histones and dextran sulfate-histone complexes did not seem to be related to the titer of anti-single stranded DNA antibody and anti-double stranded DNA antibody. We demonstrated the presence of polyanion-restricted antibodies in SLE, which may be responsible for the LE factor.
Yamashita
R
R
Second Department of Internal Medicine, Miyazaki Medical College, Kiyotake.
Tachibana
N
N
Murai
K
K
Okayama
A
A
Tsubouchi
H
H
D000293:Adolescent; D000328:Adult; D000368:Aged; D000464:Alginates / Q000032:analysis; D017153:Antibodies, Anticardiolipin / Q000032:analysis; D000974:Antibodies, Antinuclear / Q000032:analysis; D001323:Autoantibodies / Q000032:analysis*; D015415:Biomarkers / Q000032:analysis; D015153:Blotting, Western; D016264:Dextran Sulfate / Q000032:analysis; D005260:Female; D020723:Glucuronic Acid; D006603:Hexuronic Acids; D006657:Histones / Q000276:immunology*; D006801:Humans; D008180:Lupus Erythematosus, Systemic / Q000276:immunology*; D008297:Male; D008875:Middle Aged; D009504:Neutrophils / Q000276:immunology
D000464:Alginates; D017153:Antibodies, Anticardiolipin; D000974:Antibodies, Antinuclear; D001323:Autoantibodies; D015415:Biomarkers; D006603:Hexuronic Acids; D006657:Histones; D020723:Glucuronic Acid; D016264:Dextran Sulfate