title: Evaluation of non-invasive clinical samples in chronic chlamydial prostatitis by using in situ hybridization.
abstract: Seventy-eight non-invasive prostate specimens collected from patients with chronic non-bacterial prostatitis were evaluated by in situ hybridization (IH) for evidence of Chlamydia trachomatis. Intracellular Chlamydia bodies were detected in 18 of 78 cases (20.6%). Homogeneous blue-black bodies in the cellular cytoplasm were accepted as in situ positive. Chlamydial antigen detected by enzyme immuno assay (EIA) was positive in 12 cases (13.7%), but only nine of them were positive by IH. Our study confirms previous reports implicating C. trachomatis as an aetiological agent in chronic non-bacterial prostatitis, and underscores the applicability of DNA probes for detection and identification of C. trachomatis in prostatic materials.
authors.affiliation: Department of Urology, Faculty of Medicine, University of Celal Bayer, Manisa, Turkey.
authors.forename: B
authors.initials: B
authors.lastname: Gümüş
authors.forename: A Z
authors.initials: AZ
authors.lastname: Sengil
authors.forename: M
authors.initials: M
authors.lastname: Solak
authors.forename: T
authors.initials: T
authors.lastname: Fistik
authors.forename: E
authors.initials: E
authors.lastname: Alibey
authors.forename: E A
authors.initials: EA
authors.lastname: Cakmak
authors.forename: M
authors.initials: M
authors.lastname: Yeter
mesh_terms: D000328:Adult; D002690:Chlamydia Infections / Q000175:diagnosis*; D002692:Chlamydia trachomatis / Q000302:isolation & purification*; D002908:Chronic Disease; D006801:Humans; D007124:Immunoenzyme Techniques; D017403:In Situ Hybridization; D008297:Male; D011472:Prostatitis / Q000382:microbiology*