Sensitive and rapid method for the simultaneous quantification of five antidepressants with their respective metabolites in plasma using high-performance liquid chromatography with diode-array detection.
A high-performance liquid chromatographic method with diode array detection (HPLC-DAD) has been developed for the simultaneous separation and quantification of imipramine, amitriptyline, maprotiline, fluoxetine, clomipramine and their respective metabolites using a 500-microl plasma sample and clovoxamine as the internal standard. The substances were eluted on a Symmetry C18 5-microm column (250x4.6 mm, I.D.). Full UV spectra from 200 to 450 nm were recorded on-line during the entire analysis and were automatically compared to spectra stored in a library. The quantification was performed at 226, 254, and 400 nm. Peak height ratios were linear over a concentration range of 10-3000 ng/ml: the correlation coefficient (r) was better than 0.998 for all substances at each wavelength. Acceptable coefficients of variation are demonstrated for both within-run and day-to-day assays. The method is simple, highly specific and currently being used for drug monitoring and toxicological studies in children and adult patients.
Aymard
G
G
Hôpital Pitié-Salpêtrière, Division A. Paré, Paris, France.
Livi
P
P
Pham
Y T
YT
Diquet
B
B
D000639:Amitriptyline / Q000097:blood; D018687:Antidepressive Agents, Second-Generation / Q000097:blood*; D000929:Antidepressive Agents, Tricyclic / Q000097:blood*; D002851:Chromatography, High Pressure Liquid; D002997:Clomipramine / Q000097:blood; D005473:Fluoxetine / Q000097:blood; D006801:Humans; D007099:Imipramine / Q000097:blood; D008376:Maprotiline / Q000097:blood; D012680:Sensitivity and Specificity; D013056:Spectrophotometry, Ultraviolet
D018687:Antidepressive Agents, Second-Generation; D000929:Antidepressive Agents, Tricyclic; D005473:Fluoxetine; D000639:Amitriptyline; D008376:Maprotiline; D002997:Clomipramine; D007099:Imipramine