title: Role of NifS in maturation of glutamine phosphoribosylpyrophosphate amidotransferase.
abstract: Glutamine phosphoribosylpyrophosphate amidotransferase from Bacillus subtilis is synthesized as an inactive precursor that requires two maturation steps: incorporation of a [4Fe-4S] center and cleavage of an 11-residue NH2-terminal propeptide. Overproduction from a multicopy plasmid in Escherichia coli leads to the formation of soluble proenzyme and mature enzyme forms as well as a small fraction of insoluble proenzyme. Heterologous expression of Azotobacter vinelandii nifS from a compatible plasmid increased the maturation of the soluble proenzyme three- to fourfold without influencing the content of the insoluble fraction. These results support a role for NifS in heterologous Fe-S cluster assembly and enzyme maturation.
authors.affiliation: Department of Biochemistry, Purdue University, West Lafayette, Indiana 47907, USA.
authors.forename: S
authors.initials: S
authors.lastname: Chen
authors.forename: L
authors.initials: L
authors.lastname: Zheng
authors.forename: D R
authors.initials: DR
authors.lastname: Dean
authors.forename: H
authors.initials: H
authors.lastname: Zalkin
mesh_terms: D000582:Amidophosphoribosyltransferase / Q000378:metabolism*; D016948:Azotobacter vinelandii / Q000201:enzymology / Q000235:genetics; D001412:Bacillus subtilis / Q000201:enzymology; D013437:Carbon-Sulfur Lyases; D004792:Enzyme Precursors / Q000378:metabolism*; D004926:Escherichia coli / Q000235:genetics; D008190:Lyases / Q000378:metabolism*; D011994:Recombinant Proteins / Q000378:metabolism
chemical_list: D004792:Enzyme Precursors; D011994:Recombinant Proteins; D000582:Amidophosphoribosyltransferase; D008190:Lyases; D013437:Carbon-Sulfur Lyases; C080546:cysteine desulfurase