pH dependence of antibody/lysozyme complexation.
Association between proteins often depends on the pH and ionic strength conditions of the medium in which it takes place. This is especially true in complexation involving titratable residues at the complex interface. Continuum electrostatics methods were used to calculate the pH-dependent energetics of association of hen egg lysozyme with two closely related monoclonal antibodies raised against it and the association of these antibodies against an avian species variant. A detailed analysis of the energetic contributions reveals that even though the hallmark of association in the two complexes is the presence of conserved charged-residue interactions, the environment of these interactions significantly influences the titration behavior and concomitantly the energetics. The contributing factors include minor structural rearrangements, buried interfacial area, dielectric environment of the key titratable residues, and geometry of the residue dispositions. Modeled structures of several mutant complexes were also studied so as to further delineate the contribution of individual factors to the titration behavior.
Gibas
C J
CJ
Department of Molecular and Integrative Physiology, Center for Biophysics and Computational Biology, University of Illinois at Urbana-Champaign, Urbana, Illinois 61801, USA.
Subramaniam
S
S
McCammon
J A
JA
Braden
B C
BC
Poljak
R J
RJ
D000595:Amino Acid Sequence; D000818:Animals; D000911:Antibodies, Monoclonal / Q000737:chemistry / Q000235:genetics / Q000276:immunology* / Q000378:metabolism; D000936:Antigen-Antibody Complex / Q000737:chemistry* / Q000378:metabolism; D002645:Chickens; D004529:Egg White; D004563:Electrochemistry; D006860:Hydrogen Bonding; D006863:Hydrogen-Ion Concentration; D051379:Mice; D008958:Models, Molecular; D008969:Molecular Sequence Data; D009113:Muramidase / Q000737:chemistry / Q000235:genetics / Q000276:immunology* / Q000378:metabolism; D009154:Mutation; D011485:Protein Binding; D016415:Sequence Alignment; D013816:Thermodynamics; D017186:Titrimetry; D014867:Water / Q000737:chemistry / Q000378:metabolism
D000911:Antibodies, Monoclonal; D000936:Antigen-Antibody Complex; D014867:Water; D009113:Muramidase