title: Exchange of viral promoter/enhancer elements with heterologous regulatory sequences generates targeted hybrid long terminal repeat vectors for gene therapy of melanoma.
abstract: To generate transcriptionally targeted vectors, tissue-specific elements of the human tyrosinase promoter were exchanged with corresponding viral elements in the Moloney murine leukemia virus long terminal repeat (LTR). From these experiments, a vesicular stomatitis virus type G pseudotyped, hybrid LTR vector that contained three tyrosinase enhancer elements and gave high-level, tightly tissue-specific expression at high titers (3 x 10(7) CFU/ml) was constructed.
authors.affiliation: Richard Dimbleby Department of Cancer Research/ICRF Laboratory, Rayne Institute, St. Thomas' Hospital, London, United Kingdom.
authors.forename: R M
authors.initials: RM
authors.lastname: Diaz
authors.forename: T
authors.initials: T
authors.lastname: Eisen
authors.forename: I R
authors.initials: IR
authors.lastname: Hart
authors.forename: R G
authors.initials: RG
authors.lastname: Vile
mesh_terms: D000818:Animals; D004742:Enhancer Elements, Genetic; D018390:Gene Targeting; D005809:Genes, Regulator; D005814:Genes, Viral; D015316:Genetic Therapy; D005822:Genetic Vectors; D006801:Humans; D009052:Leukemia Virus, Murine / Q000235:genetics*; D008545:Melanoma / Q000628:therapy*; D051379:Mice; D014442:Monophenol Monooxygenase / Q000235:genetics; D011401:Promoter Regions, Genetic; D012091:Repetitive Sequences, Nucleic Acid; D014407:Tumor Cells, Cultured; D014721:Vesicular stomatitis Indiana virus / Q000235:genetics
chemical_list: D014442:Monophenol Monooxygenase