title: Glycosylation of RNA polymerase II from wheat germ.
abstract: RNA polymerase II from wheat germ was analyzed for the presence of sugars. The two largest subunits and the 27 and 25 kDa subunits were found to be glycosylated by a variety of sugars. However, no N-acetylglucosamine was detected, which was found by Kelly et al. (J. Biol. Chem. (1993) 268, 10416-10424) in the largest subunit of RNA polymerase II from calf thymus. Thus it appears that the regulatory function of this sugar, postulated by Kelly et al., is performed in the wheat germ enzyme by other monosaccharides. Carbohydrate analysis of the two largest subunits of the calf thymus enzyme also revealed the presence, beside N-acetylglucosamine, of other sugars. Some similarities in the features of glycosylation of the two polymerases, isolated from very different organisms, suggest that the sugar moieties have an important role in the structure and/or function of these enzymes.
authors.affiliation: Department of Biochemical Sciences, A. Rossi Fanelli and CNR Center of Molecular Biology, University La Sapienza, Rome, Italy.
authors.forename: L
authors.initials: L
authors.lastname: Cervoni
authors.forename: C
authors.initials: C
authors.lastname: Turano
authors.forename: A
authors.initials: A
authors.lastname: Ferraro
authors.forename: P
authors.initials: P
authors.lastname: Ciavatta
authors.forename: F
authors.initials: F
authors.lastname: Marmocchi
authors.forename: M
authors.initials: M
authors.lastname: Eufemi
mesh_terms: D000818:Animals; D002417:Cattle; D005690:Galactose / Q000378:metabolism; D005700:Galactosyltransferases / Q000378:metabolism; D006023:Glycoproteins / Q000378:metabolism; D006031:Glycosylation; D008970:Molecular Weight; D012319:RNA Polymerase II / Q000378:metabolism*; D013950:Thymus Gland / Q000201:enzymology; D014908:Triticum / Q000201:enzymology*
chemical_list: D006023:Glycoproteins; D005700:Galactosyltransferases; D012319:RNA Polymerase II; D005690:Galactose