Microencapsulation of human insulin DEAE-dextran complex and the complex in liposomes by the emulsion non-solvent addition method.
Human insulin-DEAE (diethyl amino ethyl) dextran complex and human insulin DEAE-dextran complex in liposomes were encapsulated in cellulose acetate butyrate (CAB) microcapsules by the emulsion non-solvent addition method. The ratio of core-to-coat used was 1:1. The average diameters of the complex microcapsules and the complex liposome microcapsules were 239.5 +/- 77.5 and 182.9 +/- 52.2 microns respectively. In vitro dissolution studies of both types of microcapsules in simulated intestinal fluid at pH 7.2 showed a sustained release of the complex and the complex liposome microcapsules with t50 = 1.5 h and 4 h respectively. This study can be applied to the further development of oral formulations of human insulin liposomes for diabetic treatment.
Manosroi
A
A
Department of Pharmaceutical Technology, Faculty of Pharmacy, Chiang Mai University, Thailand.
Manosroi
J
J
D002214:Capsules / Q000138:chemical synthesis / Q000737:chemistry; D002482:Cellulose / Q000031:analogs & derivatives / Q000737:chemistry; D003637:DEAE-Dextran / Q000737:chemistry*; D003692:Delayed-Action Preparations / Q000138:chemical synthesis / Q000737:chemistry; D004339:Drug Compounding / Q000379:methods; D004655:Emulsions; D006801:Humans; D007328:Insulin / Q000737:chemistry*; D008081:Liposomes / Q000737:chemistry* / Q000378:metabolism; D010316:Particle Size; D012997:Solvents
D002214:Capsules; D003692:Delayed-Action Preparations; D004655:Emulsions; D007328:Insulin; D008081:Liposomes; D012997:Solvents; D002482:Cellulose; C014396:cellulose acetate-butyrate; D003637:DEAE-Dextran