GC rich DNA oligonucleotides with narrow minor groove width.
Investigation of the width of the minor groove using 500 MHz NMR spectroscopy in three closely related 11-mer B-DNA duplexes shows that the minor groove is narrow in a GC rich oligonucleotide, and that a narrow minor groove is not something endemic to DNAs with persistent repetitions of adenine nucleotides (A-tract DNA). The width of the groove is dictated by local sequence contexts and independent of neighboring A-tract DNA.
Sarma
R H
RH
Institute of Biomolecular Stereodynamics, Chemistry, State University of New York at Albany, 12222, USA. rhs07@cnsvax.albany.edu
Sarma
M H
MH
Dai
L
L
Umemoto
K
K
D001482:Base Composition; D001483:Base Sequence; D003596:Cytosine; D004247:DNA / Q000737:chemistry*; D006147:Guanine; D019906:Nuclear Magnetic Resonance, Biomolecular; D009690:Nucleic Acid Conformation; D009838:Oligodeoxyribonucleotides / Q000737:chemistry*
D009838:Oligodeoxyribonucleotides; D006147:Guanine; D003596:Cytosine; D004247:DNA