title: High-level overexpression of yeast elongation factor 3 and detailed kinetic analysis using a coupled spectrophotometric assay.
authors.affiliation: Pharmaceutical Products Division, Abbott Laboratories, Abbott Park, Illinois 60064-3500, USA.
authors.forename: A V
authors.initials: AV
authors.lastname: Sarthy
authors.forename: T
authors.initials: T
authors.lastname: McGonigal
authors.forename: J O
authors.initials: JO
authors.lastname: Capobianco
authors.forename: T H
authors.initials: TH
authors.lastname: Holzman
authors.forename: K A
authors.initials: KA
authors.lastname: Walter
authors.forename: D A
authors.initials: DA
authors.lastname: Egan
authors.forename: R C
authors.initials: RC
authors.lastname: Goldman
mesh_terms: D000255:Adenosine Triphosphate / Q000378:metabolism; D000426:Alcohol Dehydrogenase / Q000235:genetics; D015153:Blotting, Western; D003001:Cloning, Molecular; D005656:Fungal Proteins; D007700:Kinetics; D010445:Peptide Elongation Factors / Q000235:genetics / Q000378:metabolism*; D011401:Promoter Regions, Genetic / Q000235:genetics; D012441:Saccharomyces cerevisiae; D029701:Saccharomyces cerevisiae Proteins; D013053:Spectrophotometry / Q000379:methods*
chemical_list: D005656:Fungal Proteins; D010445:Peptide Elongation Factors; D029701:Saccharomyces cerevisiae Proteins; C111584:YEF3 protein, S cerevisiae; D000255:Adenosine Triphosphate; D000426:Alcohol Dehydrogenase