title: Tolerance of volunteers to cyclosporine A-dilauroylphosphatidylcholine liposome aerosol.
abstract: Cyclosporine A (CsA) in liposomes of dilauroylphosphatidylcholine (DLPC), containing 118 micrograms of CsA/L of aerosol with a particle size of 1.6 to 1.7 micron diameter, was inhaled by 10 nonsmoking, normal volunteers each for 45 min. Aerosol was administered through an Aerotech II nebulizer (CIS-US, Inc., Bedford, MA) mouthpiece. Eight of the 10 volunteers had tracheal irritation and intermittent coughing following exposure. FEV1 and FVC values were mildly reduced, but returned to normal in 1 h. Blood chemical and hematologic values were unchanged at any time point after as opposed to before inhalation. Nine of the 10 volunteers later inhaled DLPC only, administered through the nebulizer mouthpiece. There was no change in FEV1 or FVC values, and there was no coughing or tracheal irritation. Subsequently, five of the volunteers who had previously had respiratory reactions inhaled CsA-DLPC liposome aerosol for 45-min, but through a mouth-only face mask. There was no tracheal irritation, coughing, or changes in spirometric measures. Blood concentrations of CsA at 15 min after the 45-min inhalation with a face mask averaged 83 +/- 42 ng/ml (mean +/- SD). At 24 h after treatment, CsA was undetectable in blood of the initial 10 volunteers. These studies indicate that CsA-DLPC liposome aerosol can be safely explored as a treatment for patients with moderately severe asthma.
authors.affiliation: Department of Microbiology and Immunology, Baylor College of Medicine, Houston, Texas 77030, USA.
authors.forename: B E
authors.initials: BE
authors.lastname: Gilbert
authors.forename: C
authors.initials: C
authors.lastname: Knight
authors.forename: F G
authors.initials: FG
authors.lastname: Alvarez
authors.forename: C
authors.initials: C
authors.lastname: Waldrep
authors.forename: J R
authors.initials: JR
authors.lastname: Rodarte
authors.forename: V
authors.initials: V
authors.lastname: Knight
authors.forename: W L
authors.initials: WL
authors.lastname: Eschenbacher
mesh_terms: D000280:Administration, Inhalation; D000328:Adult; D000336:Aerosols; D003371:Cough / Q000139:chemically induced; D016572:Cyclosporine / Q000008:administration & dosage* / Q000009:adverse effects / Q000493:pharmacokinetics; D004337:Drug Carriers; D005260:Female; D005541:Forced Expiratory Volume / Q000187:drug effects; D006801:Humans; D008081:Liposomes; D008168:Lung / Q000378:metabolism; D008297:Male; D008875:Middle Aged; D010316:Particle Size; D010713:Phosphatidylcholines; D013990:Tidal Volume / Q000187:drug effects; D014132:Trachea / Q000187:drug effects; D014797:Vital Capacity / Q000187:drug effects
chemical_list: D000336:Aerosols; D004337:Drug Carriers; D008081:Liposomes; D010713:Phosphatidylcholines; C015153:1,2-dilauroylphosphatidylcholine; D016572:Cyclosporine