Measurement of oxalate in human plasma ultrafiltrate by ion chromatography.
An improved ion chromatographic method for the measurement of oxalate in human plasma ultrafiltrate is described. Ultrafiltration was carried out using an appropriate device and procedure. Centrifugation of 0.5 ml heparin plasma at 4 degrees C for 50 min yielded water-clear ultrafiltrate in amounts allowing replicate measurements of oxalate. The specificity of the method was confirmed. The recovery of oxalate added to plasma was approximately 80%, whereas dilution of plasma, and of an oxalate-containing salt solution, resulted in falsely high values; the mechanism(s) underlying this phenomenon are insufficiently understood at present. The intra-assay precision of the method was assessed and from ten replicates of a pool plasma, the inter-assay precision from ten measurements of the same plasma on different days; the observed ranges of oxalate were 1.32-1.56 (mean 1.42) and 1.42-1.64 (mean 1.53) micromol/l, respectively. In plasma ultrafiltrate of a limited number of healthy volunteers the range of oxalate was 1.18-2.50 micromol/l, thus permitting renal oxalate handling to be studied.
Manoharan
M
M
Department of Surgery, University of Erlangen-Nürnberg, Erlangen, Germany.
Schwille
P O
PO
D000328:Adult; D002852:Chromatography, Ion Exchange; D005260:Female; D006440:Hemofiltration; D006801:Humans; D008297:Male; D008875:Middle Aged; D010070:Oxalates / Q000097:blood*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
D010070:Oxalates