Storage temperature of the unbuffered rapid urease test.
OBJECTIVE
The unbuffered rapid urease test (RUT) is an accurate, rapid, and inexpensive method for detecting Helicobacter pylori. However, it is generally recommended that the reagent be prepared daily. This prospective study was undertaken to evaluate the shelf life of our unbuffered RUT when stored at 4 and -20 degrees C.


METHODS
Ninety-five patients were studied. Three sets of antral (X2) and body (X1) biopsy samples were taken from each patient. The samples were subjected to histological examination, with the RUTs stored at 4 and -20 degrees C. The RUT tubes were examined at 1 and 15 min.


RESULTS
Fifty-six patients (59%) were infected with H. pylori as defined by histological examination. The reagent was classified according to storage time (group I, < or = 5 days; group II, > 5 days). The mean (SD) storage time of group I (n = 59) and group II (n = 36) was 3.2 (1.4) and 9.9 (5.0) days, respectively. At 15 min, the sensitivity of our RUT stored at 4 degrees C was significantly higher in group I than in group II (92 vs 47%). On the other hand, the sensitivity of our RUT stored at -20 degrees C remained consistently high in both groups (15 min: group I, 92%; group II, 100%). Our RUTs stored at 4 and -20 degrees C were highly specific in both groups.


CONCLUSIONS
Our RUT remains highly sensitive and specific when it is stored at 4 degrees C for up to 5 days. When the RUT is expected to be stored for a longer period of time, the bottles should be frozen at -20 degrees C.
Ng
F H
FH
Department of Medicine, Ruttonjee Hospital, Hong Kong.
Wong
S Y
SY
Ng
W F
WF
D001706:Biopsy; D002021:Buffers; D003080:Cold Temperature; D004356:Drug Storage; D005069:Evaluation Studies as Topic; D005615:Freezing; D016481:Helicobacter Infections / Q000175:diagnosis* / Q000473:pathology; D016480:Helicobacter pylori / Q000201:enzymology; D006801:Humans; D007202:Indicators and Reagents; D011446:Prospective Studies; D011706:Pyloric Antrum / Q000382:microbiology / Q000473:pathology; D011933:Reagent Kits, Diagnostic; D012680:Sensitivity and Specificity; D013270:Stomach / Q000382:microbiology / Q000473:pathology; D013997:Time Factors; D014508:Urea / Q000378:metabolism; D014510:Urease / Q000378:metabolism
D002021:Buffers; D007202:Indicators and Reagents; D011933:Reagent Kits, Diagnostic; D014508:Urea; D014510:Urease