Rapid detection of Staphylococcus saprophyticus using primer specific PCR.
Staphylococcus saprophyticus is one of the most frequently encountered clinically significant members of the coagulase-negative staphylococci. A set of species-specific PCR primers was defined for the detection of Staphylococcus saprophyticus. These primers target variable regions (V3 and V6) of the 16S rRNA gene. Primer-specific PCR has potential applications in epidemiological studies and diagnosis of Staphylococcus saprophyticus.
Gaszewska-Mastalarz
A
A
Ludwik Hirszfeld Institute of Immunology and Experimental Therapy, Polish Academy of Sciences, Wrocław, Poland.
Zakrzewska-Czerwinska
J
J
Mordarski
M
M
D001431:Bacteriological Techniques; D001483:Base Sequence; D017931:DNA Primers / Q000235:genetics; D005069:Evaluation Studies as Topic; D005798:Genes, Bacterial; D014644:Genetic Variation; D006801:Humans; D017720:Molecular Epidemiology; D016133:Polymerase Chain Reaction / Q000379:methods*; D012329:RNA, Bacterial / Q000235:genetics; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D013045:Species Specificity; D013203:Staphylococcal Infections / Q000175:diagnosis / Q000453:epidemiology; D013210:Staphylococcus / Q000235:genetics* / Q000302:isolation & purification* / Q000472:pathogenicity
D017931:DNA Primers; D012329:RNA, Bacterial; D012336:RNA, Ribosomal, 16S