Lectin histochemical HPA-binding pattern of human breast and colon cancers is associated with metastases formation in severe combined immunodeficient mice.
Metastasis formation is a major clinical problem in cancer treatment, and no significant progress in the treatment of metastatic spread has been made. This apparent lack of progress is partly caused by the absence of clinically relevant animal models of metastases. The binding of the lectin Helix pomatia agglutinin (HPA) has been associated with a poor prognosis in breast and colon cancer patients. HPA-positive and -negative human breast and colon cancer cell lines were transplanted into severe combined immunodeficient (SCID) mice. HPA-positive breast cancer cell lines (MCF-7 and T47D) metastasized in SCID mice, whereas the HPA-negative ones (BT20, HS578T and HBL100) did not. The HPA-positive colon cancer cell line HT29 metastasized, while the HPA-negative ones (COLO320DM, SW480 and SW620) did not. However, in two of eight SCID mice inoculated with the HPA-negative colon cancer cell line, CACO2 metastatic deposits were found. Despite this exception, HPA binding is a good indicator of the metastasis of human breast and colon cancer cells in SCID mice: 23 out of 26 HPA-positive cancers metastasized, as opposed to only two out of 38 HPA-negative cancers. This experimental model is well suited for investigating the functional role of carbohydrate residues recognized by HPA in breast and colon cancer metastasis.
Schumacher
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U
University of Southampton, UK.
Adam
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E
D000818:Animals; D001665:Binding Sites; D001943:Breast Neoplasms / Q000378:metabolism*; D003110:Colonic Neoplasms / Q000378:metabolism*; D006372:Helix, Snails; D006388:Hemagglutinins / Q000378:metabolism*; D006651:Histocytochemistry / Q000379:methods*; D006801:Humans; D037102:Lectins / Q000737:chemistry / Q000378:metabolism; D008175:Lung Neoplasms / Q000378:metabolism / Q000556:secondary*; D051379:Mice; D016513:Mice, SCID; D014407:Tumor Cells, Cultured / Q000378:metabolism
C038095:Helix lectin; D006388:Hemagglutinins; D037102:Lectins