title: DNA-Binding properties of the replication telomere protein.
abstract: The replication Telomere Protein, rTP, is a nuclear protein from the ciliate Euplotes crassus that appears to be a novel telomere replication factor. rTP shares extensive amino acid sequence identity with the two proteins that bind and protect the macronuclear telomeres from the ciliates Oxytricha and Euplotes. Since the most extended regions of conservation fall within the DNA-binding domains of the telomere-binding proteins, when rTP was first identified it was predicted to be another structural telomere-binding protein. However, subsequent research demonstrated that rTP transcripts accumulate only during DNA replication and the rTP protein localizes to the sites of DNA replication within Euplotes macronuclei. We have now expressed rTP in a heterologous expression system and have examined the DNA-binding properties of the recombinant protein. We show that rTP binds specifically to the G-strand of Euplotes telomeric DNA and hence has some of the same DNA-binding characteristics as the Euplotes and Oxytricha telomere-binding proteins. However, other aspects of rTP binding are unique. In particular, the protein exhibits a very high off-rate and can bind double-stranded DNA as well as internal tracts of telomeric sequence. We conclude that rTP and the telomere-binding proteins are members of a class of proteins that have a conserved DNA-binding motif tailored to bind the G-strand of telomeric DNA. However, the unique DNA-binding characteristics of rTP indicate that the protein has evolved to fulfil a specialized role during telomere replication.
authors.affiliation: Department of Chemistry, University of Nebraska, Lincoln, Nebraska 68588, USA.
authors.forename: D L
authors.initials: DL
authors.lastname: Carlson
authors.forename: R
authors.initials: R
authors.lastname: Skopp
authors.forename: C M
authors.initials: CM
authors.lastname: Price
mesh_terms: D000818:Animals; D001483:Base Sequence; D001667:Binding, Competitive; D015153:Blotting, Western; D017124:Conserved Sequence / Q000235:genetics; D016054:DNA, Protozoan / Q000378:metabolism*; D004268:DNA-Binding Proteins / Q000378:metabolism*; D003854:Deoxyribonucleotides / Q000737:chemistry / Q000378:metabolism; D016812:Euplotes / Q000737:chemistry; D019143:Evolution, Molecular; D008969:Molecular Sequence Data; D016813:Oxytricha / Q000737:chemistry; D015800:Protozoan Proteins; D011994:Recombinant Proteins / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000235:genetics; D016615:Telomere / Q000235:genetics / Q000378:metabolism
chemical_list: D016054:DNA, Protozoan; D004268:DNA-Binding Proteins; D003854:Deoxyribonucleotides; D015800:Protozoan Proteins; D011994:Recombinant Proteins; C101550:rTP protein, protozoa