Role of inositol 1,4,5-trisphosphate receptor in ventral signaling in Xenopus embryos.
The inositol 1,4,5-trisphosphate (IP3) receptor is a calcium ion channel involved in the release of free Ca2+ from intracellular stores. For analysis of the role of IP3-induced Ca2+ release (IICR) on patterning of the embryonic body, monoclonal antibodies that inhibit IICR were produced. Injection of these blocking antibodies into the ventral part of early Xenopus embryos induced modest dorsal differentiation. A close correlation between IICR blocking potencies and ectopic dorsal axis induction frequency suggests that an active IP3-Ca2+ signal may participate in the modulation of ventral differentiation.
Kume
S
S
Mikoshiba Calciosignal Net Project, Exploratory Research for Advanced Technology (ERATO), Japan Science and Technology Corporation (JST), 2-9-3 Shimo-Meguro, Meguro-ku, Tokyo 153, Japan. skume@ims.u-tokyo.ac.jp
Muto
A
A
Inoue
T
T
Suga
K
K
Okano
H
H
Mikoshiba
K
K
D028341:Activins; D000818:Animals; D019138:Antibodies, Blocking; D000911:Antibodies, Monoclonal; D019521:Body Patterning; D002118:Calcium / Q000378:metabolism*; D015220:Calcium Channels / Q000276:immunology / Q000378:metabolism*; D002454:Cell Differentiation; D004625:Embryo, Nonmammalian / Q000378:metabolism*; D047108:Embryonic Development; D004627:Embryonic Induction; D016222:Fibroblast Growth Factor 2 / Q000494:pharmacology; D005775:Gastrula / Q000378:metabolism; D018507:Gene Expression Regulation, Developmental; D007265:Inhibins / Q000494:pharmacology; D015544:Inositol 1,4,5-Trisphosphate / Q000378:metabolism*; D053496:Inositol 1,4,5-Trisphosphate Receptors; D018160:Receptors, Cytoplasmic and Nuclear / Q000276:immunology / Q000378:metabolism*; D015398:Signal Transduction; D014981:Xenopus
D019138:Antibodies, Blocking; D000911:Antibodies, Monoclonal; D015220:Calcium Channels; D053496:Inositol 1,4,5-Trisphosphate Receptors; D018160:Receptors, Cytoplasmic and Nuclear; D016222:Fibroblast Growth Factor 2; D028341:Activins; D007265:Inhibins; D015544:Inositol 1,4,5-Trisphosphate; D002118:Calcium