title: Crystallization and preliminary X-ray analysis of a truncated family A alkaline endoglucanase isolated from Bacillus sp. KSM-635.
abstract: The catalytic domain of an alkaline endo-1,4-beta-glucanase (family A) isolated from Bacillus sp. KSM-635 (Mr = 40.2 kDa) was crystallized using the hanging drop vapor diffusion method. Two different crystal forms were obtained. Form 2 crystals (trigonal space group R3 with cell dimensions of a = b = 111.9 and c = 207.1 angstroms in a hexagonal lattice) were found to be more stable than form 1 ones upon X-ray irradiation. A full data set for form 2 crystals has been collected up to 3.3 angstroms resolution.
authors.affiliation: Department of Biotechnology and Biomaterial Chemistry, Graduate School of Engineering, Nagoya University.
authors.forename: T
authors.initials: T
authors.lastname: Shirai
authors.forename: T
authors.initials: T
authors.lastname: Yamane
authors.forename: T
authors.initials: T
authors.lastname: Hidaka
authors.forename: K
authors.initials: K
authors.lastname: Kuyama
authors.forename: A
authors.initials: A
authors.lastname: Suzuki
authors.forename: T
authors.initials: T
authors.lastname: Ashida
authors.forename: K
authors.initials: K
authors.lastname: Ozaki
authors.forename: S
authors.initials: S
authors.lastname: Ito
mesh_terms: D000595:Amino Acid Sequence; D001407:Bacillus / Q000201:enzymology*; D002384:Catalysis; D002480:Cellulase / Q000737:chemistry* / Q000378:metabolism; D018360:Crystallography, X-Ray; D008969:Molecular Sequence Data; D017386:Sequence Homology, Amino Acid
chemical_list: D002480:Cellulase