New biomarker evidence of oxidative DNA damage in patients with non-insulin-dependent diabetes mellitus.
Urinary 8-hydroxy-2'-deoxyguanosine (8-OHdG) has been reported to serve as a sensitive biomarker of oxidative DNA damage and also of oxidative stress. We have investigated oxidative DNA damage in patients with non-insulin-dependent diabetes mellitus (NIDDM) by urinary 8-OHdG assessments. We determined the total urinary excretion of 8-OHdG from 24 h urine samples of 81 NIDDM patients 9 years after the initial diagnosis and of 100 non-diabetic control subjects matched for age and gender. The total 24 h urinary excretion of 8-OHdG was markedly higher in NIDDM patients than in control subjects (68.2 +/- 39.4 microg vs. 49.6 +/- 37.7 microg, P = 0.001). High glycosylated hemoglobin was associated with a high level of urinary 8-OHdG. The increased excretion of urinary 8-OHdG is seen as indicating an increased systemic level of oxidative DNA damage in NIDDM patients.
Leinonen
J
J
Laboratory of Neurobiology, Medical School, University of Tampere, Finland.
Lehtimäki
T
T
Toyokuni
S
S
Okada
K
K
Tanaka
T
T
Hiai
H
H
Ochi
H
H
Laippala
P
P
Rantalaiho
V
V
Wirta
O
O
Pasternack
A
A
Alho
H
H
D000080242:8-Hydroxy-2'-Deoxyguanosine; D000368:Aged; D015415:Biomarkers / Q000652:urine; D001786:Blood Glucose / Q000378:metabolism; D004249:DNA Damage; D003849:Deoxyguanosine / Q000031:analogs & derivatives* / Q000378:metabolism / Q000652:urine; D003924:Diabetes Mellitus, Type 2 / Q000378:metabolism / Q000652:urine*; D005260:Female; D006442:Glycated Hemoglobin / Q000378:metabolism; D006801:Humans; D008297:Male; D008875:Middle Aged; D018384:Oxidative Stress; D012737:Sex Factors; D012907:Smoking
D000080242:8-Hydroxy-2'-Deoxyguanosine; D015415:Biomarkers; D001786:Blood Glucose; D003849:Deoxyguanosine; D006442:Glycated Hemoglobin