Differential internalisation of mGluR1 splice variants in response to agonist and phorbol esters in permanently transfected BHK cells.
The internalisation of metabotropic glutamate receptor (mGluR1alpha) and its splice variant (mGluR1beta), in response to agonist and phorbol esters (PMA), has been studied. Both mGluR1alpha and mGluR1beta exhibit a similar rate of internalisation following PMA treatment, with a shift in their distribution from plasma membrane to endosome-enriched membrane fractions. Agonist challenge however caused a rapid loss, within 5-10 min, of mGluR1beta but not mGluR1alpha from the cell surface. These results show that the two forms of mGluR1 show different internalisation responses to agonist and suggest that the C-terminal region of the molecule plays an important role in this phenomenon.
Ciruela
F
F
Medical Research Council Anatomical Neuropharmacology Unit, Oxford, UK.
McIlhinney
R A
RA
D017398:Alternative Splicing; D000818:Animals; D002460:Cell Line; D002462:Cell Membrane / Q000378:metabolism; D006224:Cricetinae; D011992:Endosomes / Q000378:metabolism; D014644:Genetic Variation; D007668:Kidney; D007700:Kinetics; D018094:Receptors, Metabotropic Glutamate / Q000096:biosynthesis* / Q000378:metabolism; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis / Q000378:metabolism; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology; D014162:Transfection
D018094:Receptors, Metabotropic Glutamate; D011993:Recombinant Fusion Proteins; C104077:metabotropic glutamate receptor type 1; D013755:Tetradecanoylphorbol Acetate