Inhibition of proteases, myotoxins and phospholipases A2 from Bothrops venoms by the heteromeric protein complex of Didelphis albiventris opossum serum.
The antibothropic complex (ABC) from opossum (species Didelphis albiventris) serum was purified by chromatography on DEAE-Sephacel. It showed an acidic character and two polypeptide chains of ca. 45 kDa and 48 kDa, respectively. Lyophilized opossum serum or the ABC (100 micrograms), as well as ethylenediamine tetraacetate (0.25 mumoles) were able to completely neutralise the hemorrhagic effect of 50 micrograms of the desiccated venoms of Bothrops moojeni, Bothrops pirajai and Bothrops jararacussu. The myotoxic (100 micrograms venom in mice) and edematogenic (90 micrograms venom in rats) activities of Bothrops moojeni and Bothrops jararacussu venoms, as well as of the major myonecrotic protein (myotoxin-I) isolated from Bothrops moojeni venom, were also totally inhibited by the ABC (200 micrograms and 270 micrograms, respectively). The lyophilized opossum serum (30 micrograms) and the ABC (30 micrograms) reduced to 50% the phospholipase A2 activity of Bothrops moojeni venom (10 micrograms). The clotting activity of Bothrops alternatus and Bothrops moojeni (20 micrograms) on bovine plasma was also significantly inhibited by the ABC (60 micrograms).
Soares
A M
AM
Departamento de Bioquímica, Faculdade de Medicina, Universidade de São Paulo, Ribeirão Preto, Brazil.
Rodrigues
V M
VM
Borges
M H
MH
Andrião-Escarso
S H
SH
Cunha
O A
OA
Homsi-Brandeburgo
M I
MI
Giglio
J R
JR
D000818:Animals; D000997:Antivenins / Q000097:blood* / Q000494:pharmacology*; D001681:Biological Assay; D001780:Blood Coagulation Tests; D017837:Bothrops; D003435:Crotalid Venoms / Q000037:antagonists & inhibitors* / Q000201:enzymology; D004487:Edema / Q000139:chemically induced / Q000517:prevention & control; D006470:Hemorrhage / Q000139:chemically induced / Q000517:prevention & control; D008297:Male; D051379:Mice; D009893:Opossums / Q000097:blood; D010741:Phospholipases A / Q000037:antagonists & inhibitors*; D054467:Phospholipases A2; D011480:Protease Inhibitors / Q000494:pharmacology*; D051381:Rats; D017208:Rats, Wistar
D000997:Antivenins; D003435:Crotalid Venoms; D011480:Protease Inhibitors; D010741:Phospholipases A; D054467:Phospholipases A2