title: Purification, staphylolytic activity, and cleavage sites of alpha-lytic protease from Achromobacter lyticus.
abstract: Alpha-lytic protease (alp) was purified from a bacteriolytic agent, Achromopeptidase from Achromobacter lyticus M497-1, and has been shown to possess staphylolytic activity. Cleavage sites of this enzyme on the peptidoglycan of Staphylococcus aureus were determined by N-terminal amino acid sequence and amino acid composition analyses. Alp cleaved the N-acetylmuramoyl-L-alanine amide bond, the junction between the polysaccharide and peptide moieties, in addition to the D-Ala-Gly and Gly-Gly peptide bonds, implying that this enzyme recognizes the amino acid of D-configuration at the P1 site and possesses N-acetylmuramoyl-L-alanine amidase activity. However, alp could not cleave the D-Ala-Gly peptide bond in a synthetic peptide, suggesting that this hydrolytic activity of alp is peptidoglycan-specific. The results obtained from different consecutive actions of alp and glycosidase on S. aureus peptidoglycan indicate that the presence of polysaccharide in the peptidoglycan is necessary for the bacteriolytic activity of alp.
authors.affiliation: Division of Protein Chemistry, Institute for Protein Research, Osaka University, Suita. lishao@protein.osaka-u.ac.jp
authors.forename: S
authors.initials: S
authors.lastname: Li
authors.forename: S
authors.initials: S
authors.lastname: Norioka
authors.forename: F
authors.initials: F
authors.lastname: Sakiyama
mesh_terms: D000421:Alcaligenes / Q000302:isolation & purification* / Q000378:metabolism; D000595:Amino Acid Sequence; D000900:Anti-Bacterial Agents / Q000302:isolation & purification* / Q000378:metabolism / Q000494:pharmacology; D002851:Chromatography, High Pressure Liquid; D004591:Electrophoresis, Polyacrylamide Gel; D006868:Hydrolysis; D008969:Molecular Sequence Data; D011487:Protein Conformation; D012697:Serine Endopeptidases / Q000302:isolation & purification* / Q000378:metabolism / Q000494:pharmacology; D013211:Staphylococcus aureus / Q000187:drug effects*; D013379:Substrate Specificity
chemical_list: D000900:Anti-Bacterial Agents; D012697:Serine Endopeptidases; C021826:myxobacter alpha-lytic proteinase