Induction of Th2 cytokine expression for p27-specific IgA B cell responses after targeted lymph node immunization with simian immunodeficiency virus antigens in rhesus macaques.
To determine if there is an association between the isotype of simian immunodeficiency virus (SIV)-specific B cell responses and the profile of Th1 and Th2 cytokine expression, rhesus macaques were immunized with SIV antigens via the iliac lymph nodes, using a targeted lymph node (TLN) immunization procedure. When CD4+ T cells purified from antigen-stimulated peripheral blood mononuclear cells were analyzed, the levels of Th2 cytokine production were gradually increased after the second and third immunizations. However, interferon-gamma production did not change. Analysis of SIV-specific B cell responses revealed that the main isotype was IgG after the second and third immunizations. In addition, a peak of SIV-specific IgA B cell responses was noted following the third immunization. These findings suggest that the induction of Th2 type responses in TLN-immunized rhesus macaques reflects the sequence of initial induction of SIV-specific IgG-producing cells followed by IgA-secreting cells.
Kawabata
S
S
Department of Oral Biology, Immunobiology Vaccine Center, University of Alabama at Birmingham, USA.
Miller
C J
CJ
Lehner
T
T
Fujihashi
K
K
Kubota
M
M
McGhee
J R
JR
Imaoka
K
K
Hiroi
T
T
Kiyono
H
H
D000818:Animals; D000914:Antibodies, Viral / Q000032:analysis / Q000276:immunology; D000956:Antigens, Viral / Q000276:immunology; D001402:B-Lymphocytes / Q000276:immunology* / Q000378:metabolism; D015704:CD4 Antigens / Q000276:immunology; D002478:Cells, Cultured; D016207:Cytokines / Q000032:analysis / Q000235:genetics / Q000378:metabolism*; D005260:Female; D005434:Flow Cytometry; D015870:Gene Expression; D015683:Gene Products, gag / Q000276:immunology*; D015699:HIV Envelope Protein gp120 / Q000276:immunology; D007070:Immunoglobulin A / Q000378:metabolism*; D007074:Immunoglobulin G / Q000378:metabolism; D007371:Interferon-gamma / Q000235:genetics / Q000378:metabolism; D007376:Interleukin-2 / Q000235:genetics / Q000378:metabolism; D015847:Interleukin-4 / Q000235:genetics / Q000378:metabolism; D015850:Interleukin-6 / Q000235:genetics / Q000378:metabolism; D007963:Leukocytes, Mononuclear / Q000276:immunology; D008198:Lymph Nodes / Q000276:immunology / Q000821:virology; D008253:Macaca mulatta; D008562:Membrane Glycoproteins; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000032:analysis / Q000235:genetics; D015302:Simian Immunodeficiency Virus / Q000276:immunology*; D018417:Th1 Cells / Q000276:immunology / Q000378:metabolism; D018418:Th2 Cells / Q000276:immunology* / Q000378:metabolism; D014611:Vaccination; D014759:Viral Envelope Proteins
D000914:Antibodies, Viral; D000956:Antigens, Viral; D015704:CD4 Antigens; D016207:Cytokines; C066594:Gag protein p27, Simian immunodeficiency virus; D015683:Gene Products, gag; D015699:HIV Envelope Protein gp120; D007070:Immunoglobulin A; D007074:Immunoglobulin G; D007376:Interleukin-2; D015850:Interleukin-6; D008562:Membrane Glycoproteins; D012333:RNA, Messenger; D014759:Viral Envelope Proteins; C069623:gp120 protein, Simian immunodeficiency virus; D015847:Interleukin-4; D007371:Interferon-gamma