title: Liquid chromatography-atmospheric pressure ionization mass spectrometry for the determination of chloro- and nitrophenolic compounds in tap water and sea water.
abstract: Liquid chromatography coupled to atmospheric-pressure ionization mass spectrometry (LC-API-MS) with negative ion detection was studied for the determination of a variety of phenolic compounds in environmental waters. An isocratic mobile phase of 0.05% acetic acid-acetonitrile (50:50, v/v) was used. The influence of post-column addition of different bases on the sensitivity of the detection in electrospray (ES) was studied. The [M-H]-ion was the base peak for all the compounds using both ES and atmospheric-pressure chemical-ionization (APCI) ion sources. Moreover, abundant structural information was obtained by increasing the extraction voltage. Detection limits for standard solutions ranging from 2 to 13 ng injected for LC-ES-MS and from 0.02 to 20 ng for LC-APCI-MS were obtained. Good reproducibilities (day-to-day and run-to-run) were observed. The optimum LC-ES-MS and LC-APCI-MS conditions thus determined were used for a quantitative analysis of some phenolic compounds in spiked tap water and sea water samples.
authors.affiliation: Departament de Química Analítica, Universitat de Barcelona, Spain.
authors.forename: O
authors.initials: O
authors.lastname: Jáuregui
authors.forename: E
authors.initials: E
authors.lastname: Moyano
authors.forename: M T
authors.initials: MT
authors.lastname: Galceran
mesh_terms: D002733:Chlorophenols / Q000032:analysis*; D002853:Chromatography, Liquid; D013058:Mass Spectrometry; D009596:Nitrophenols / Q000032:analysis*; D015203:Reproducibility of Results; D012623:Seawater / Q000737:chemistry*; D012680:Sensitivity and Specificity; D014867:Water / Q000737:chemistry*; D014874:Water Pollutants, Chemical / Q000032:analysis*
chemical_list: D002733:Chlorophenols; D009596:Nitrophenols; D014874:Water Pollutants, Chemical; D014867:Water