Controlled release of leukaemia inhibitory factor (LIF) to tissues.
Leukaemia inhibitory factor (LIF) has been shown to effectively enhance skeletal muscle regeneration after mechanical injury and it may have potential therapeutic use in the muscular dystrophies as well as peripheral nerve repair after injury. When LIF is applied systemically to an animal, it is rapidly removed with a biological half life of only a few minutes, and at high doses it exhibits toxic effects. Calcium alginate rods have been developed for the purpose of insertion adjacent to skeletal muscles. These rods, when charged with LIF will release the growth factor to the muscle at a rate of less than 1% per day and for a period extending to several months. In addition, tubes of alginate are described which will be suitable for the continuous supply of LIF to repaired peripheral nerve.
Austin
L
L
Melbourne Neuromuscular Research Centre, St Vincent's Hospital, Australia. austinl@mail4.svhm.org.au
Bower
J J
JJ
Kurek
J B
JB
Muldoon
C M
CM
D000464:Alginates; D000818:Animals; D004337:Drug Carriers; D016189:Dystrophin / Q000378:metabolism; D020723:Glucuronic Acid; D006131:Growth Inhibitors / Q000493:pharmacokinetics* / Q000494:pharmacology / Q000627:therapeutic use; D006603:Hexuronic Acids; D015850:Interleukin-6; D007457:Iodine Radioisotopes; D053605:Leukemia Inhibitory Factor; D008222:Lymphokines / Q000493:pharmacokinetics* / Q000494:pharmacology / Q000627:therapeutic use; D051379:Mice; D008810:Mice, Inbred C57BL; D018482:Muscle, Skeletal / Q000187:drug effects / Q000293:injuries / Q000378:metabolism*; D011107:Polylysine / Q000494:pharmacology; D011994:Recombinant Proteins / Q000493:pharmacokinetics / Q000494:pharmacology / Q000627:therapeutic use
D000464:Alginates; D004337:Drug Carriers; D016189:Dystrophin; D006131:Growth Inhibitors; D006603:Hexuronic Acids; D015850:Interleukin-6; D007457:Iodine Radioisotopes; D053605:Leukemia Inhibitory Factor; C507838:Lif protein, mouse; D008222:Lymphokines; D011994:Recombinant Proteins; D011107:Polylysine; D020723:Glucuronic Acid