title: Antimycobacterial polyynes of Devil's Club (Oplopanax horridus), a North American native medicinal plant.
abstract: Two new (3 and 5), as well as three known (1, 2, and 4), polyynes were isolated from Devil's Club (Oplopanax horridus; Araliaceae), a medicinal plant of North America. The structures were established by 1H and 13C NMR. The absolute configurations of 2 and 5 were determined by application of Mosher's method. All the polyynes exhibited significant anti-Candida, antibacterial, and antimycobacterial activity, with an ability to kill Mycobacterium tuberculosis and isoniazid-resistant Mycobacterium avium at 10 micrograms/disk in a disk diffusion assay.
authors.affiliation: Botany and Microbiology, University of British Columbia, Vancouver, Canada.
authors.forename: M
authors.initials: M
authors.lastname: Kobaisy
authors.forename: Z
authors.initials: Z
authors.lastname: Abramowski
authors.forename: L
authors.initials: L
authors.lastname: Lermer
authors.forename: G
authors.initials: G
authors.lastname: Saxena
authors.forename: R E
authors.initials: RE
authors.lastname: Hancock
authors.forename: G H
authors.initials: GH
authors.lastname: Towers
authors.forename: D
authors.initials: D
authors.lastname: Doxsee
authors.forename: R W
authors.initials: RW
authors.lastname: Stokes
mesh_terms: D000900:Anti-Bacterial Agents / Q000302:isolation & purification* / Q000494:pharmacology; D000995:Antitubercular Agents / Q000494:pharmacology; D004352:Drug Resistance, Microbial; D007538:Isoniazid / Q000494:pharmacology; D008826:Microbial Sensitivity Tests; D009161:Mycobacterium / Q000187:drug effects*; D009162:Mycobacterium avium / Q000187:drug effects; D009169:Mycobacterium tuberculosis / Q000187:drug effects; D019441:Plant Epidermis / Q000737:chemistry; D010946:Plants, Medicinal / Q000737:chemistry*; D011090:Polyenes / Q000302:isolation & purification* / Q000494:pharmacology; D013055:Spectrophotometry, Infrared
chemical_list: D000900:Anti-Bacterial Agents; D000995:Antitubercular Agents; D011090:Polyenes; D007538:Isoniazid