title: Synthesis of asymmetrically labeled sucrose by a recombinant sucrose synthase.
abstract: About 80% of radioactivity was recovered in asymmetrically labeled sucrose from UDP-[14C]glucose or [14C]fructose with recombinant mung bean sucrose synthase expressed in Escherichia coli harboring pEB-01. This high recovery is due to the fact that the enzyme conserving the activity of sucrose synthase has a similar affinity for UDP-glucose and fructose to an intact enzyme from the mung bean, but a lower affinity for sucrose.
authors.affiliation: Wood Research Institute, Kyoto University, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Nakai
authors.forename: N
authors.initials: N
authors.lastname: Tonouchi
authors.forename: T
authors.initials: T
authors.lastname: Tsuchida
authors.forename: H
authors.initials: H
authors.lastname: Mori
authors.forename: F
authors.initials: F
authors.lastname: Sakai
authors.forename: T
authors.initials: T
authors.lastname: Hayashi
mesh_terms: D002250:Carbon Radioisotopes / Q000737:chemistry; D004926:Escherichia coli / Q000201:enzymology / Q000235:genetics; D005632:Fructose / Q000737:chemistry / Q000378:metabolism; D005964:Glucosyltransferases / Q000235:genetics / Q000378:metabolism*; D007553:Isotope Labeling / Q000379:methods; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism; D013395:Sucrose / Q000138:chemical synthesis* / Q000378:metabolism; D014532:Uridine Diphosphate Glucose / Q000737:chemistry / Q000378:metabolism
chemical_list: D002250:Carbon Radioisotopes; D011994:Recombinant Proteins; D005632:Fructose; D013395:Sucrose; D005964:Glucosyltransferases; C022699:sucrose synthase; D014532:Uridine Diphosphate Glucose