A cryptic protease activity from Trypanosoma cruzi revealed by preincubation with kininogen at low temperatures.
Cysteine proteases have been identified in parasitic protozoa including the causative agent of Chagas' disease Trypanosoma cruzi. T. cruzi lysates subjected to substrate-containing SDS-polyacrylamide gel electrophoresis exhibit major bands of proteolytic activity in the 45-55 kDa molecular mass range (cruzipain activity). Paradoxically, addition of kininogen (a cystatin-like protease inhibitor) to the lysates before electrophoresis results in the appearance of additional bands of proteolytic activity in the 160-190 kDa molecular mass range. This inhibitor-activated protease activity depends upon the reaction conditions and exhibits novel properties. For example, a 24-48 hour preincubation at low temperature (-20 degrees C optimum) greatly enhances the proteolytic activity. The results suggest that a metastable complex forms between kininogen and a cryptic 30 kDa cysteine protease from T. cruzi and that this complex participates in the activation of proteolytic activity.
Wiser
M F
MF
Department of Tropical Medicine, Tulane University School of Public Health and Tropical Medicine, New Orleans, Louisiana 70112, USA. wiser@mailhost.tcs.tulane.edu
Lonsdale-Eccles
J D
JD
D'Alessandro
A
A
Grab
D J
DJ
D000818:Animals; D003546:Cysteine Endopeptidases / Q000378:metabolism*; D004591:Electrophoresis, Polyacrylamide Gel; D004789:Enzyme Activation; D004791:Enzyme Inhibitors / Q000494:pharmacology; D005780:Gelatin / Q000378:metabolism; D007700:Kinetics; D007704:Kininogens / Q000378:metabolism* / Q000494:pharmacology; D008970:Molecular Weight; D013696:Temperature; D014349:Trypanosoma cruzi / Q000201:enzymology*
D004791:Enzyme Inhibitors; D007704:Kininogens; D005780:Gelatin; D003546:Cysteine Endopeptidases