Targeting the secretory pathway of Toxoplasma gondii.
Little is known about the extent of conservation in the organization of the secretory pathway in organisms as different as prokaryotes, eukaryotes, and humans. The protozoan parasite Toxoplasma gondii allows easy genetic manipulations, and numerous vectors for selection of transgenic parasites have been developed. One approach to study the molecular mechanism of protein sorting and trafficking is the expression of foreign proteins. Here we describe the design and application of a vector that targets proteins to the secretory pathway of T. gondii and yields high-level expression of Escherichia coli reporter proteins. The general strategies and potential problems in expressing foreign proteins in T. gondii are discussed.
Karsten
V
V
Section of Infectious Diseases, LCI 808, Yale University School of Medicine, 333 Cedar Street, New Haven, Connecticut, 06520-8022, USA.
Qi
H
H
Beckers
C J
CJ
Joiner
K A
KA
D000595:Amino Acid Sequence; D000818:Animals; D030801:Animals, Genetically Modified; D019677:Artificial Gene Fusion / Q000379:methods; D002522:Chlorocebus aethiops; D003001:Cloning, Molecular / Q000379:methods; D004926:Escherichia coli / Q000235:genetics; D018014:Gene Transfer Techniques; D017930:Genes, Reporter; D005822:Genetic Vectors; D006801:Humans; D018688:Neospora / Q000235:genetics; D016271:Proto-Oncogene Proteins c-myc / Q000096:biosynthesis; D015800:Protozoan Proteins / Q000096:biosynthesis*; D016324:Sequence Tagged Sites; D014122:Toxoplasma / Q000235:genetics* / Q000502:physiology; D014162:Transfection / Q000379:methods; D014709:Vero Cells; D001618:beta-Lactamases / Q000096:biosynthesis
D016271:Proto-Oncogene Proteins c-myc; D015800:Protozoan Proteins; D001618:beta-Lactamases