The relationship between brain temperature and neutrophil accumulation after traumatic brain injury in rats.
Mild hypothermia reduces secondary damage after traumatic brain injury (TBI) in rodent models; however, the mechanisms involved in this beneficial effect remain unclear. We previously reported that TBI induces the upregulation of adhesion molecules and infiltration of neutrophils (PMN) in brain. Since PMN accumulation may be associated with the development of hyperemia and blood-brain barrier injury, we hypothesized that hypothermia would reduce acute inflammation after TBI in rats. To test this hypothesis, rats were anesthetized and subjected to TBI by controlled cortical impact to left parietal cortex. Brain temperature was controlled at 32 degrees C, 37 degrees C, or 39 degrees C (n = 8 per group) for 4 h after TBI, then rats were sacrificed and brain were harvested. Immunohistochemistries were performed on brain sections using antibodies that recognize the adhesion molecules E-selectin and intercellular adhesion molecule-1 (ICAM-1), and PMN. PMN were also quantified using a myeloperoxidase (MPO) assay. PMN accumulation in injured brain was decreased in rats maintained at 32 degrees C vs 39 degrees C (4-fold by immunohistochemistry and 8-fold by MPO, p < 0.05). E-selectin was induced after TBI, but not attenuated by hypothermia. ICAM-1 was not up-regulated at this early time after TBI. Based on these preliminary data, we conclude that mild hypothermia reduces PMN accumulation in injured brain during the initial 4 h after TBI, without decreasing adhesion molecule expression.
Whalen
M J
MJ
Safar Center for Resuscitation Research, University of Pittsburgh, PA, USA.
Carlos
T M
TM
Clark
R S
RS
Marion
D W
DW
DeKosky
M S
MS
Heineman
S
S
Schiding
J K
JK
Memarzadeh
F
F
Dixon
C E
CE
Kochanek
P M
PM
D000818:Animals; D001930:Brain Injuries / Q000097:blood / Q000503:physiopathology*; D007036:Hypothermia, Induced; D007958:Leukocyte Count; D009504:Neutrophils / Q000166:cytology*; D051381:Rats; D017207:Rats, Sprague-Dawley