title: Drosophila NURF-55, a WD repeat protein involved in histone metabolism.
abstract: The Drosophila nucleosome remodeling factor (NURF) is a protein complex of four distinct subunits that assists transcription factor-mediated chromatin remodeling. One NURF subunit, ISWI, is related to the transcriptional regulators Drosophila brahma and yeast SWI2/SNF2. We have determined peptide sequences and isolated cDNA clones for a second NURF component (the 55-kDa subunit). Immunological studies show that p55 is an integral subunit of NURF and is generally associated with polytene chromosomes. The predicted sequence of p55 reveals a WD repeat protein that is identical with the 55-kDa subunit of the Drosophila chromatin assembly factor (CAF-1). Given that WD repeat proteins related to p55 are associated with histone deacetylase and histone acetyltransferase, our findings suggest that p55 and its homologs may function as a common platform for the assembly of protein complexes involved in chromatin metabolism.
authors.affiliation: Laboratory of Molecular Cell Biology, National Cancer Institute, Building 37, Room 5E-26, National Institutes of Health, Bethesda, MD 20892-4255, USA.
authors.forename: M A
authors.initials: MA
authors.lastname: Martínez-Balbás
authors.forename: T
authors.initials: T
authors.lastname: Tsukiyama
authors.forename: D
authors.initials: D
authors.lastname: Gdula
authors.forename: C
authors.initials: C
authors.lastname: Wu
mesh_terms: D000123:Acetyltransferases / Q000378:metabolism; D000595:Amino Acid Sequence; D000818:Animals; D002843:Chromatin / Q000378:metabolism; D056504:Chromatin Assembly Factor-1; D002868:Chromosomal Proteins, Non-Histone; D004268:DNA-Binding Proteins / Q000737:chemistry / Q000378:metabolism*; D004330:Drosophila; D029721:Drosophila Proteins; D019084:Fluorescent Antibody Technique, Indirect; D051548:Histone Acetyltransferases; D006657:Histones / Q000378:metabolism*; D019476:Insect Proteins / Q000737:chemistry / Q000378:metabolism*; D018832:Molecular Chaperones; D008969:Molecular Sequence Data; D009687:Nuclear Proteins; D056505:Retinoblastoma-Binding Protein 4; D029701:Saccharomyces cerevisiae Proteins
chemical_list: C110251:Caf1-55 protein, Drosophila; D002843:Chromatin; D056504:Chromatin Assembly Factor-1; D002868:Chromosomal Proteins, Non-Histone; D004268:DNA-Binding Proteins; D029721:Drosophila Proteins; D006657:Histones; D019476:Insect Proteins; D018832:Molecular Chaperones; D009687:Nuclear Proteins; D056505:Retinoblastoma-Binding Protein 4; D029701:Saccharomyces cerevisiae Proteins; D000123:Acetyltransferases; D051548:Histone Acetyltransferases