Evidence for rolling circle replication of Autographa californica M nucleopolyhedrovirus genomic DNA.
Autographa californica M nucleopolyhedrovirus (AcMNPV) is a large ds DNA virus restricted to larval lepidopteran insect hosts. Using field inversion gel electrophoresis and digestion with a restriction enzyme which cuts the AcMNPV genome once, we detected multiple unit-length genome fragments from replicating viral DNA. Our data suggest that AcMNPV replicates in a head-to-tail manner via rolling circle replication.
Oppenheimer
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Department of Plant and Microbial Biology, University of California, Berkeley, USA.
Volkman
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LE
D000818:Animals; D002460:Cell Line; D004261:DNA Replication; D004279:DNA, Viral / Q000096:biosynthesis*; D016679:Genome, Viral; D009036:Moths / Q000821:virology; D017924:Nucleopolyhedroviruses / Q000235:genetics* / Q000502:physiology; D015183:Restriction Mapping; D018411:Spodoptera / Q000166:cytology; D014779:Virus Replication
D004279:DNA, Viral