Tissue suspension agglutination: a simple method to screen species-specific and organ-specific reactions.
Agglutination tests with preparations of parenchymatous organs were developed. The tissue suspensions were dried at room temperature after they had been spread as a very thin layer on a glass plate, or otherwise, they were lyophilized. The dried preparations were pulverized and then prepared as stable suspensions in saline. The agglutination test was conducted on a slide by mixing one drop of the tested serum at a convenient dilution with one drop of tissue powder suspension. Agglutination in the form of readily discernible clumps could be assessed after 1-10 min. By means of this procedure, species-specific reactions were studied using suspensions of kidneys of various species. Organ-specific reactions were noted with suspensions of brain and thyroid. Agglutination of thyroid powder was observed with rabbit anti-rabbit thyroid sera as well as with many, albeit not all, sera of patients with Hashimoto's disease.
Milgrom
F
F
Department of Microbiology, School of Medicine, State University of New York at Buffalo, 14214, USA.
Czechowski
D
D
Colombani
J
J
Marcelli
A
A
D000372:Agglutination Tests / Q000379:methods*; D000818:Animals; D001921:Brain / Q000276:immunology; D002417:Cattle; D006801:Humans; D007668:Kidney / Q000276:immunology; D009928:Organ Specificity; D051381:Rats; D013045:Species Specificity; D013961:Thyroid Gland / Q000276:immunology; D013967:Thyroiditis, Autoimmune / Q000276:immunology