An effective method of completely removing contaminating genomic DNA from an RNA sample to be used for PCR.
A simple method for removing contaminating genomic DNA from an RNA preparation is presented. The method involves digestion of the RNA with RNase-free DNase I at room temperature followed by inactivation of the enzyme at 65 degrees C in presence of EDTA. This method produces an RNA sample that is negative for genomic DNA by PCR.
Sanyal
A
A
Department of Orthopedics, Mayo Clinic, Rochester, MN 55905, USA.
O'Driscoll
S W
SW
Bolander
M E
ME
Sarkar
G
G
D001671:Biochemistry / Q000379:methods*; D004247:DNA / Q000737:chemistry*; D018076:DNA, Complementary; D003850:Deoxyribonuclease I / Q000737:chemistry; D004492:Edetic Acid / Q000737:chemistry; D016133:Polymerase Chain Reaction / Q000379:methods*; D012313:RNA / Q000302:isolation & purification*
D018076:DNA, Complementary; D012313:RNA; D004247:DNA; D004492:Edetic Acid; D003850:Deoxyribonuclease I