title: [Genetic analysis of two cellular degenerations in filamentous fungus Podospora anserina].
abstract: The filamentous fungus Podopsora anserina presents an unavoidable arrest of vegetative growth (Senescence) determined by a cytoplasmic and infectious factor. Senescence is correlated with a disorganization of the mitochondrial DNA. This disorganization is caused by an event which is not the appearance of the first defective DNA molecules. These ones are generated constitutively and their accumulation during Senescence requires the presence of an additional factor. Life span of the strains is under nuclear and cytoplasmic genetic control. At least 600 nuclear genes influence longevity. Our analysis focuses on the role of the genes involved in cytosolic translation, since mutations in these genes seem to display the most drastic effects on longevity but also on the structure of the defective mitochondrial DNA molecules that accumulate during Senescence. We have detected in some Podospora anserina mutant strains (permissive strains) the presence of a novel cytoplasmic and infectious determinant that entails an easily discernible phenotype associated with a severe growth alteration (Crippled Growth). This growth alteration is not associated with mitochondrial DNA modifications. Only the strains that have an increased translational accuracy present Crippled Growth. However, the Crippled Growth Determinant is found in all the strains during the stationary phase; it is eliminated from the non permissive strains during the exit of the stationary phase. The mutants, that have an increased translational accuracy, probably lack a factor which is needed to eliminate the determinant when cells enter the growth phase.
authors.affiliation: Laboratoire Contrôle Génétique de Dégénérescences Cellulaires, Institut de Génétique et Microbiologie, URA CNRS 2225, Université de Paris Sud, Orsay.
authors.forename: P
authors.initials: P
authors.lastname: Silar
authors.forename: C
authors.initials: C
authors.lastname: Vierny
authors.forename: B
authors.initials: B
authors.lastname: Gagny
authors.forename: M
authors.initials: M
authors.lastname: Rossignol
authors.forename: V
authors.initials: V
authors.lastname: Haedens
mesh_terms: D001203:Ascomycota / Q000235:genetics* / Q000254:growth & development / Q000502:physiology*; D002467:Cell Nucleus / Q000235:genetics; D016922:Cellular Senescence; D004271:DNA, Fungal / Q000235:genetics; D004272:DNA, Mitochondrial / Q000235:genetics
chemical_list: D004271:DNA, Fungal; D004272:DNA, Mitochondrial