title: Soluble receptors to tumour necrosis factor and interleukin-6 in urine during acute pyelonephritis.
abstract: We compared the urinary concentrations of soluble TNF-I (sTNF-RI), TNF-II receptors, and soluble IL-6 receptor (sIL-6R) standardized to urinary creatinine concentrations, in children with acute pyelonephritis, in children with non-renal fever and in healthy controls. These levels were related to the acute inflammatory response in the kidneys and later renal scarring, as determined by acute and 1-y follow-up with 99mTC-dimercaptosuccinic acid scintigraphy (DMSA). The concentrations of the soluble receptors were measured using enzyme immunoassay (EIA). The urinary levels of sTNF-RI were significantly higher in children with acute pyelonephritis (median 1320 pg/mmol) than in children with non-renal fever, children 6 weeks after acute pyelonephritis and healthy controls (873, 251 and 477 pg/mumol, respectively). Median sTNF-RII urine levels were also higher in acute pyelonephritis (4123 pg/mumol) than in the three control groups (2000, 964 and 1850 pg/mumol, respectively). In contrast, the highest urinary sIL-6R concentrations were found in healthy children (median 420 pg/mumol), compared to those with acute pyelonephritis (235 pg/mumol), children with non-renal fever and children 6 weeks after pyelonephritis (137 and 50 pg/mumol, respectively). No significant difference was found in any of the urinary soluble receptor levels in children with or without DMSA uptake defects at the acute or the 1-y follow-up scintigraphy. In conclusion, although the urinary soluble TNF receptor levels were higher during acute pyelonephritis, this observation was not useful for deciding which children needed follow-up after acute pyelonephritis.
authors.affiliation: Department of Woman and Child Health, St Göran's Children's Hospital, Karolinska Institute, Stockholm, Sweden.
authors.forename: K
authors.initials: K
authors.lastname: Tullus
authors.forename: R
authors.initials: R
authors.lastname: Escobar-Billing
authors.forename: O
authors.initials: O
authors.lastname: Fituri
authors.forename: Y
authors.initials: Y
authors.lastname: Lu
authors.forename: A
authors.initials: A
authors.lastname: Brauner
mesh_terms: D000208:Acute Disease; D002675:Child, Preschool; D006801:Humans; D007223:Infant; D015850:Interleukin-6 / Q000652:urine*; D011704:Pyelonephritis / Q000276:immunology / Q000652:urine*; D018121:Receptors, Cytokine / Q000302:isolation & purification*; D012016:Reference Values; D012995:Solubility; D014409:Tumor Necrosis Factor-alpha / Q000652:urine*
chemical_list: D015850:Interleukin-6; D018121:Receptors, Cytokine; D014409:Tumor Necrosis Factor-alpha