title: Possible contribution of follicular interleukin-1beta to nitric oxide generation in human pre-ovulatory follicles.
abstract: The aim of this study was to investigate the relationships between follicular nitric oxide (NO) metabolite concentrations and several related variables, with special reference to follicular interleukin-1beta (IL-1beta). The follicular fluid from the leading and secondary follicles was collected individually from 20 women undergoing in-vitro fertilization (IVF) treatment, and the concentrations of nitrite (NO2-) and nitrate (NO3-) were determined fluorometrically using 2,3-diaminonaphthalene. Both follicular nitrite (r = 0.42, P < 0.01) and nitrate (r = 0.49, P < 0.001) were found to be significantly correlated with follicular IL-1beta concentrations. There were also significant positive correlations between follicular nitrate and the number of oocytes retrieved (P < 0.01) and serum oestradiol concentration on the day of human chorionic gonadotrophin (HCG) administration (P < 0.05). When follicular cells were incubated in vitro with 10 ng/ml of IL-1beta for 24 h, nitrate generation was significantly (P < 0.01) elevated compared with the control. In conclusion, our study demonstrates that follicular IL-1beta and the number of developing follicles are significant variables that affect follicular NO concentrations, and points to the possible contribution of IL-1beta to NO generation in human preovulatory follicles.
authors.affiliation: Department of Obstetrics and Gynecology, Akita University School of Medicine, Japan.
authors.forename: M
authors.initials: M
authors.lastname: Tao
authors.forename: H
authors.initials: H
authors.lastname: Kodama
authors.forename: S
authors.initials: S
authors.lastname: Kagabu
authors.forename: J
authors.initials: J
authors.lastname: Fukuda
authors.forename: M
authors.initials: M
authors.lastname: Murata
authors.forename: Y
authors.initials: Y
authors.lastname: Shimizu
authors.forename: H
authors.initials: H
authors.lastname: Hirano
authors.forename: T
authors.initials: T
authors.lastname: Tanaka
mesh_terms: D015081:2-Naphthylamine / Q000031:analogs & derivatives; D000328:Adult; D002452:Cell Count; D006063:Chorionic Gonadotropin / Q000008:administration & dosage; D004958:Estradiol / Q000032:analysis / Q000097:blood; D005260:Female; D005307:Fertilization in Vitro; D005456:Fluorescent Dyes; D015571:Follicular Fluid / Q000737:chemistry / Q000378:metabolism*; D006801:Humans; D007375:Interleukin-1 / Q000032:analysis / Q000378:metabolism*; D009566:Nitrates / Q000032:analysis; D009569:Nitric Oxide / Q000378:metabolism*; D009573:Nitrites / Q000032:analysis; D009865:Oocytes; D006080:Ovarian Follicle / Q000378:metabolism*; D010060:Ovulation; D011374:Progesterone / Q000032:analysis; D013050:Spectrometry, Fluorescence
chemical_list: D006063:Chorionic Gonadotropin; D005456:Fluorescent Dyes; D007375:Interleukin-1; D009566:Nitrates; D009573:Nitrites; C026373:2,3-diaminonaphthalene; D009569:Nitric Oxide; D011374:Progesterone; D004958:Estradiol; D015081:2-Naphthylamine