The RNA binding region of the paramyxovirus SV5 V and P proteins.
The V/P gene of simian virus 5 (SV5) encodes two proteins, V (222 residues) and the phosphoprotein P (392 residues). The V and P proteins are amino coterminal for 164 residues, but they have unique carboxy termini due to addition of two nontemplated G residues to the P mRNA during transcription. We have shown that the V and P proteins bind RNA by using both Northwestern blot analysis and ultraviolet-light crosslinking. The RNA-binding region has been mapped to a region in the P and V proteins which contains five basic residues (K74, K76, K77, R79, K81). Either deletion of the basic residues or substitution of the basic residues with alanine inhibited RNA binding by the V or P proteins.
Lin
G Y
GY
Department of Biochemistry, Molecular Biology and Cell Biology, Northwestern University, Evanston, Illinois 60208-3500, USA.
Paterson
R G
RG
Lamb
R A
RA
D000595:Amino Acid Sequence; D000818:Animals; D001665:Binding Sites; D015152:Blotting, Northern; D015153:Blotting, Western; D002417:Cattle; D002460:Cell Line; D002874:Chromosome Mapping; D003432:Cross-Linking Reagents; D008969:Molecular Sequence Data; D010750:Phosphoproteins; D016601:RNA-Binding Proteins / Q000235:genetics / Q000378:metabolism*; D011817:Rabbits; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000378:metabolism; D018108:Respirovirus / Q000378:metabolism*; D014466:Ultraviolet Rays; D014764:Viral Proteins / Q000235:genetics / Q000378:metabolism*; D015678:Viral Structural Proteins
D003432:Cross-Linking Reagents; C055859:P protein, Sendai virus; D010750:Phosphoproteins; D016601:RNA-Binding Proteins; D011993:Recombinant Fusion Proteins; C097019:V protein, Simian parainfluenza virus 5; D014764:Viral Proteins; D015678:Viral Structural Proteins