Ribotyping and random amplification of polymorphic DNA for nosocomial Enterobacter cloacae isolates in a pediatric intensive-care unit.
Between 1987 and 1989, two sequential outbreaks of nosocomial infection caused by Enterobacter cloacae occur-red in the pediatric intensive-care unit of a tertiary-care teaching hospital. Seventeen strains retrieved from the outbreaks and two control strains identified in other wards were typed by ribotyping and random amplification of polymorphic DNA (RAPD). The results indicated that the genomic pattern of strains identified between the first and second outbreaks was different. We conclude that both ribotyping and RAPD are highly discriminatory and reproducible methods for typing E cloacae. RAPD seems to be more efficacious and cost-effective.
Hou
S T
ST
Department of Pediatrics, Tri-Service General Hospital, Taipei City, Taiwan, Republic of China.
Wang
C C
CC
Chu
M L
ML
D015373:Bacterial Typing Techniques; D003428:Cross Infection / Q000453:epidemiology / Q000382:microbiology*; D004269:DNA, Bacterial / Q000032:analysis; D004196:Disease Outbreaks; D016972:Enterobacter cloacae / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D004756:Enterobacteriaceae Infections / Q000453:epidemiology / Q000382:microbiology; D006801:Humans; D015278:Intensive Care Units, Pediatric; D012335:RNA, Ribosomal / Q000032:analysis; D019105:Random Amplified Polymorphic DNA Technique
D004269:DNA, Bacterial; D012335:RNA, Ribosomal