Enzymatic large-scale production of 2-keto-3-deoxy-D-glycero-D-galacto-nonopyranulosonic acid in enzyme membrane reactors.
The enzymatic synthesis of 2-keto-3-deoxy-D-glycero-D-galacto-nonopyranulosonic acid (KDN) starting from D-mannose and pyruvic acid using Neu5Ac-aldolase has been scaled up. A repetitive batch ultrafiltration bioreactor was used for the KDN synthesis on 100 g scale with a conversion of up to 85%. Furthermore, a 440 mL pilot-scale enzyme membrane reactor (EMR) was performed for the continuous production of KDN. Conversion of mannose was 75% at a space--time yield of 375 g/(L d). KDN was advanteageously isolated by crystallization with an overall yield of 75%.
Salagnad
C
C
Forschungszentrum Jülich GmbH, Institut für Biotechnologie, Jülich, Germany.
Gödde
A
A
Ernst
B
B
Kragl
U
U
D019149:Bioreactors; D005634:Fructose-Bisphosphate Aldolase; D008358:Mannose / Q000378:metabolism; D008567:Membranes, Artificial; D007652:Oxo-Acid-Lyases / Q000378:metabolism*; D019289:Pyruvic Acid / Q000378:metabolism; D013400:Sugar Acids / Q000378:metabolism*
D008567:Membranes, Artificial; D013400:Sugar Acids; D019289:Pyruvic Acid; C050008:3-deoxyglycero-galacto-nonulosonic acid; D005634:Fructose-Bisphosphate Aldolase; D007652:Oxo-Acid-Lyases; C018728:N-acetylneuraminate lyase; D008358:Mannose