Effects of catechols on free radical formation by chemotherapeutic agents (adriamycin, farmorubicin, and mitomycin).
The aim of the present study was to investigate the effects of biologically important catechols on the cytotoxicity of adriamycin, farmorubicin, and mitomycin C with respect to hydroxyl radical production. Catecholamines (adrenalin, noradrenaline, dopamine) and DOPA enhance the generation of hydroxyl radicals by chemotherapeutic antibiotics. Measures were done using a deoxyribose assay, in presence of the Co(II) + H2O2 system. Catalase and hydroxyl radical scavengers (mannitol, thiourea, cysteine, glutathione, L-lactic dehydrogenase) inhibited the deoxyribose damage caused by the drugs.
Bounias
M
M
Department of Phytopharmacy and Ecotoxicology, University of Avignon, Versailles, France.
Kladny
J
J
Kruk
I
I
Michalska
T
T
Lichszteld
K
K
D000903:Antibiotics, Antineoplastic / Q000737:chemistry* / Q000494:pharmacology; D002395:Catecholamines / Q000737:chemistry*; D003855:Deoxyribose / Q000737:chemistry; D004317:Doxorubicin / Q000737:chemistry* / Q000494:pharmacology; D015251:Epirubicin / Q000737:chemistry* / Q000494:pharmacology; D006801:Humans; D017665:Hydroxyl Radical / Q000737:chemistry*; D016685:Mitomycin / Q000737:chemistry* / Q000494:pharmacology; D016877:Oxidants
D000903:Antibiotics, Antineoplastic; D002395:Catecholamines; D016877:Oxidants; D017665:Hydroxyl Radical; D015251:Epirubicin; D016685:Mitomycin; D003855:Deoxyribose; D004317:Doxorubicin