An in vitro 1H-MRS model of oncogene transfection. The spectral feature of c-erbB-2 and c-Ha-ras transfected NIH3T3 fibroblast cells.
OBJECTIVE
Malignancy is an abnormality of cell division and differentiation based on abnormal expression of oncogenes. This note describes the in vitro 1H-NMR spectral features of oncogene-transfected NIH3T3 fibroblast cells compared to non-transfected cells.


METHODS
1H-NMR spectra of cultured NIH3T3 cells and c-erbB-2 or c-Ha-ras gene-transfected cells were obtained by 400 MHz high resolution NMR. The peaks were assigned by 2D HOHAHA spectra of the cell suspension and the spectral changes were evaluated in 1D and 1D differential spectra.


RESULTS
The 1H spectra obtained from both transfected cell lines were broadened over all peaks, suggesting reduced mobility in plasma membrane lipid molecules. No other differential spectra for characterizing metabolic change was detected.


CONCLUSIONS
Broadened 1H spectra observed after c-erbB-2 or c-Ha-ras transfection suggest changes of plasma membrane viscosity, which may be related to the oncogene expression.
Nakai
T
T
Electrotec Laboratories, Supermolecular Division, Tsukuba, Japan.
Ishima
R
R
Sakahara
H
H
Endo
K
K
Konishi
J
J
Akasaka
K
K
D016475:3T3 Cells / Q000166:cytology*; D000818:Animals; D002454:Cell Differentiation / Q000235:genetics; D002455:Cell Division / Q000235:genetics; D002794:Choline / Q000032:analysis / Q000235:genetics; D005786:Gene Expression Regulation; D015972:Gene Expression Regulation, Neoplastic; D018734:Genes, erbB-2 / Q000235:genetics*; D011905:Genes, ras / Q000235:genetics*; D018698:Glutamic Acid / Q000032:analysis / Q000235:genetics; D005973:Glutamine / Q000032:analysis / Q000235:genetics; D006859:Hydrogen; D009682:Magnetic Resonance Spectroscopy; D008563:Membrane Lipids / Q000032:analysis / Q000235:genetics; D051379:Mice; D009857:Oncogenes / Q000235:genetics*; D010713:Phosphatidylcholines / Q000032:analysis / Q000235:genetics; D010767:Phosphorylcholine / Q000032:analysis; D013057:Spectrum Analysis; D014162:Transfection / Q000235:genetics*; D014783:Viscosity
D008563:Membrane Lipids; D010713:Phosphatidylcholines; D005973:Glutamine; D010767:Phosphorylcholine; D018698:Glutamic Acid; D006859:Hydrogen; D002794:Choline