Hemagglutination test for the diagnosis of human neurocysticercosis: development of a stable reagent using homologous and heterologous antigens.
A hemagglutination (HA) test was standardized using formalin- and tannin-treated gander red blood cells sensitized with a total salt extract of C. cellulosae (HA-Cc) and an antigenic extract of Cysticercus longicollis (HA-Cl) vesicular fluid. A total of 61 cerebrospinal fluid (CSF) samples were assayed, 41 from patients with neurocysticercosis and 20 from a control group which were, respectively, reactive and non-reactive to ELISA using C. cellulosae. The CSF samples from the control group did not react and 35 (85.4%) and 34 (82.9%) CSF samples from patients were reactive to the HA-Cc and HA-Cl tests, respectively. The reagents ready for use were stable up to 6 months when stored at 4 degrees C in 50% glycerol. The present results confirm that the reagent using Cysticercus longicollis stabilized with glycerol can be used as an alternative in the immunological diagnosis of neurocysticercosis.
Ferreira
A P
AP
Faculty of Pharmaceutical Sciences, University of São Paulo, Brasil.
Vaz
A J
AJ
Nakamura
P M
PM
Sasaki
A T
AT
Ferreira
A W
AW
Livramento
J A
JA
D000818:Animals; D000947:Antigens, Helminth / Q000276:immunology; D003551:Cysticercosis / Q000134:cerebrospinal fluid / Q000175:diagnosis* / Q000276:immunology; D005260:Female; D006386:Hemagglutination Tests / Q000379:methods*; D006801:Humans; D051379:Mice; D008807:Mice, Inbred BALB C
D000947:Antigens, Helminth