Substance P endopeptidase activity in the rat spinal cord following injury: influence of the new anti-oxidant compound H 290/51.
The influence of the new antioxidant compound H-290/51 was examined on the substance P endopeptidase (SPE) activity in a rat model of spinal cord injury. This compound (H-290/51) has neuro-protective effects on edema and cell changes in this model. Infliction of trauma to the cord by making an incision into the right dorsal horn of the T10-11 segment resulted in a marked upregulation of SPE in the segments rostral to the lesion. On the other hand, the injured and adjacent caudal segments exhibited a marked down-regulation of the enzyme activity. Pretreatment with H 290/51 increased the SPE activity in the T9 segment but downregulated the enzyme activity in the T10-11 and T12 segments. The drug induced enzyme activity change was not further influenced by the trauma of the cord. The results indicate that a focal trauma induces widespread alterations in spinal cord SPE activity which can be influenced by the anti-oxidant drug H 290/51, suggesting that SPE is somehow involved in cell injury.
Thörnwall
M
M
Department of Pharmaceutical Biosciences, University Hospital, Uppsala University, Sweden.
Sharma
H S
HS
Gordh
T
T
Sjöquist
P O
PO
Nyberg
F
F
D000818:Animals; D000975:Antioxidants / Q000627:therapeutic use*; D007211:Indoles / Q000627:therapeutic use*; D008666:Metalloendopeptidases / Q000378:metabolism*; D018696:Neuroprotective Agents / Q000627:therapeutic use*; D018384:Oxidative Stress / Q000187:drug effects; D011863:Radioimmunoassay; D051381:Rats; D017207:Rats, Sprague-Dawley; D012016:Reference Values; D012680:Sensitivity and Specificity; D013116:Spinal Cord / Q000201:enzymology*; D013119:Spinal Cord Injuries / Q000201:enzymology*
D000975:Antioxidants; C088233:H290-51; D007211:Indoles; D018696:Neuroprotective Agents; D008666:Metalloendopeptidases; C029610:substance P degrading enzyme