Characterization of antigen-antibody complexes by size-exclusion chromatography coupled with low-angle light-scattering photometry and viscometry.
In this paper, the molecular masses (M(r)s) of the complexes of monoclonal anti-BSA (antibody to bovine serum albumin) (clone: 33) and monomer BSA were determined on-line by using size-exclusion chromatography (SEC) coupled with a low-angle laser light-scattering (LALLS) detector and two concentration detectors, ultraviolet (UV) and refractive index (RI) (SEC-LALLS/UV/RI system). Also, the size and M(r)s of the complexes were evaluated by the SEC-LALLS/UV/viscometer (VISC) system. This study demonstrated that, for small size macromolecules, the combination of light scattering and viscosity detection was a suitable choice for determining their M(r)s and sizes.
Qian
R L
RL
Department of Chemistry, Northeastern University, Boston, MA 02115, USA.
Mhatre
R
R
Krull
I S
IS
D000911:Antibodies, Monoclonal / Q000737:chemistry* / Q000276:immunology; D000936:Antigen-Antibody Complex / Q000737:chemistry*; D002850:Chromatography, Gel; D007834:Lasers; D008027:Light; D008970:Molecular Weight; D012031:Refractometry; D012542:Scattering, Radiation; D012710:Serum Albumin, Bovine / Q000737:chemistry* / Q000276:immunology; D013056:Spectrophotometry, Ultraviolet; D014783:Viscosity
D000911:Antibodies, Monoclonal; D000936:Antigen-Antibody Complex; D012710:Serum Albumin, Bovine