title: Enhancement of human parainfluenza virus-induced cell fusion by pradimicin, a low molecular weight mannose-binding antibiotic.
abstract: Oligosaccharides, especially mannose residues, expressed on the cell surface, are thought to be important for virus-induced membrane fusion. We examined the effect of mannose-binding compounds, pradimicin derivative BMY-28,864 (PRM) and concanavalin A (Con A), on cell fusion of human parainfluenza type 2 virus (hPIV2)-infected HeLa cells. Syncytium formation of hPIV2-infected HeLa cells was suppressed in the presence of Con A. On the other hand, PRM enhanced cell fusion of hPIV2-infected HeLa cells. These effects were blocked by addition of mannose-rich mannan. However, PRM shows little effect on virus growth and the expression of viral glycoproteins on the cell surface in hPIV2-infected HeLa cells. Fluorescein-isothiocyanate-labeled pradimicin and Con A bound to both uninfected and hPIV2-infected mononuclear cells, indicating that these compounds have an affinity to several cellular component(s). In contrast to Con A, PRM had little affinity to the viral glycoproteins. It is inferred from these results that the enhancement of hPIV2-induced cell fusion is probably due to the interaction between PRM and cellular component(s).
authors.affiliation: Department of Microbiology, Mie University School of Medicine, Japan.
authors.forename: K
authors.initials: K
authors.lastname: Okamoto
authors.forename: T
authors.initials: T
authors.lastname: Oki
authors.forename: Y
authors.initials: Y
authors.lastname: Igarashi
authors.forename: M
authors.initials: M
authors.lastname: Tsurudome
authors.forename: M
authors.initials: M
authors.lastname: Nishio
authors.forename: M
authors.initials: M
authors.lastname: Kawano
authors.forename: H
authors.initials: H
authors.lastname: Komada
authors.forename: M
authors.initials: M
authors.lastname: Ito
authors.forename: Y
authors.initials: Y
authors.lastname: Sakakura
authors.forename: Y
authors.initials: Y
authors.lastname: Ito
mesh_terms: D018943:Anthracyclines; D000903:Antibiotics, Antineoplastic / Q000378:metabolism / Q000494:pharmacology*; D002352:Carrier Proteins / Q000378:metabolism; D002459:Cell Fusion / Q000187:drug effects; D003208:Concanavalin A / Q000494:pharmacology; D006367:HeLa Cells; D006801:Humans; D007700:Kinetics; D008351:Mannans / Q000494:pharmacology; D008358:Mannose / Q000378:metabolism; D010223:Parainfluenza Virus 2, Human / Q000187:drug effects* / Q000254:growth & development / Q000502:physiology*; D014764:Viral Proteins / Q000378:metabolism; D000095643:Pradimicins and Benanomicins
chemical_list: D018943:Anthracyclines; D000903:Antibiotics, Antineoplastic; D002352:Carrier Proteins; D003208:Concanavalin A; D008351:Mannans; D008358:Mannose; D014764:Viral Proteins; C065439:pradimicin D; D000095643:Pradimicins and Benanomicins; C067714:N,N-dimethylpradimicin FA-2; C066544:pradimicin FA 2