Detection of glycosaminoglycans as a copper(II) complex in high-performance liquid chromatography.
Glycosaminoglycans including heparin, heparan sulfate, chondroitin sulfate, dermatan sulfate and hyaluronan were analyzed by high-performance gel filtration chromatography. Detection was achieved at 240 nm based on the formation of copper(II) complex in copper sulfate solution at low pH. Detection of the copper(II)-heparin complex is sensitive, permitting the analysis of as little as 10(-7) g. This method was also successfully applied to the analysis of the chemically derivatized glycosaminoglycans (de-N-acetylated and/or oversulfated chondroitin sulfate) that cannot be depolymerized by enzymes such as heparin and chondroitin lyases.
Toida
T
T
Faculty of Pharmaceutical Sciences, Chiba University, Japan.
Shima
M
M
Azumaya
S
S
Maruyama
T
T
Toyoda
H
H
Imanari
T
T
Linhardt
R J
RJ
D002614:Chelating Agents / Q000032:analysis* / Q000737:chemistry; D002851:Chromatography, High Pressure Liquid; D003300:Copper / Q000737:chemistry*; D006025:Glycosaminoglycans / Q000032:analysis* / Q000737:chemistry; D006863:Hydrogen-Ion Concentration; D012680:Sensitivity and Specificity; D013056:Spectrophotometry, Ultraviolet
D002614:Chelating Agents; D006025:Glycosaminoglycans; D003300:Copper