Analysis of the antidiabetic drug acarbose by capillary electrophoresis.
This study describes the derivatization of the pseudooligosaccharide acarbose and its main metabolite, component 2, with 7-aminonaphthalene-1,3-disulfonic acid (ANDS) in human urine. Their efficient separation was possible by means of capillary zone electrophoresis, using a capillary tube of fused-silica containing 100 mM triethylammonium phosphate buffer, pH 1.5. On column laser-induced fluorescence allowed the detection of the pseudooligosaccharides in human urine in the nanomolar range. With this method, acarbose and component 2 were quantified in human urine after application of 300 mg of acarbose.
Rethfeld
I
I
Institute of Pharmaceutical Chemistry, University of Münster, Germany.
Blaschke
G
G
D020909:Acarbose; D019075:Electrophoresis, Capillary; D006801:Humans; D007004:Hypoglycemic Agents / Q000737:chemistry / Q000378:metabolism / Q000652:urine*; D007834:Lasers; D009282:Naphthalenesulfonates / Q000737:chemistry; D012680:Sensitivity and Specificity; D013050:Spectrometry, Fluorescence; D014312:Trisaccharides / Q000737:chemistry / Q000378:metabolism / Q000652:urine*
D007004:Hypoglycemic Agents; D009282:Naphthalenesulfonates; D014312:Trisaccharides; C019152:3-azido-2,7-naphthalene disulfonate; D020909:Acarbose