title: Reorientation of large DNA molecules in concentrated polyacrylamide solution during crossed-field electrophoresis.
abstract: Recently, we found that, in concentrated neutral solutions, DNA molecules migrate in linear conformation under steady electric field. In this paper, we report the conformational change of DNA during 120 degree crossed-field electrophoresis in the same polymer solution. We found that, in concentrated polyacrylamide solutions, the reorientation process of DNAs becomes simple: the DNA goes back along the previous track and the reorientation time is longer for larger DNA. Such a backtrack motion has been thought to be an essential motion for the separation of DNA fragments in pulsed field gel electrophoresis. We expect that this phenomenon is useful for a more efficient separation technique of large DNAs than the current pulsed field gel electrophoresis.
authors.affiliation: School of Engineering, Nagoya University, Japan. oana@stat.nuap.nagoya-u.ac.jp
authors.forename: H
authors.initials: H
authors.lastname: Oana
authors.forename: M
authors.initials: M
authors.lastname: Doi
authors.forename: M
authors.initials: M
authors.lastname: Ueda
authors.forename: K
authors.initials: K
authors.lastname: Yoshikawa
mesh_terms: D000180:Acrylic Resins; D017122:Bacteriophage T4 / Q000235:genetics; D010582:Bacteriophage lambda / Q000235:genetics; D004279:DNA, Viral / Q000737:chemistry* / Q000302:isolation & purification; D004586:Electrophoresis / Q000379:methods*; D008970:Molecular Weight; D009690:Nucleic Acid Conformation; D012996:Solutions; D000099166:Polyacrylamides
chemical_list: D000180:Acrylic Resins; D004279:DNA, Viral; D012996:Solutions; D000099166:Polyacrylamides