title: Preservation of pig liver allografts after warm ischemia: normothermic perfusion versus cold storage.
abstract: Warm ischemia is known to induce substantial damage to the liver parenchyma. With respect to clinical liver transplantation, the tolerance of the liver to warm ischemia and the preservation of these organs have not been studied in detail. In isolated reperfused pig livers we proceeded according to the following concept: Livers were subjected to 1 or 3 h of warm ischemia. Subsequently, these organs were preserved by either normothermic perfusion or cold storage (histidine-tryptophan-alpha-ketoglutarate, HTK) for 3 h each. After storage, liver function was assessed in a reperfusion circuit for another 3 h. Parameters under evaluation were bile flow, perfusion flow, oxygen consumption, enzyme release into the perfusate (creatine kinase, glutamic oxaloacetic transaminase (GOT), lactic dehydrogenase, and glutamic pyruvic transaminase), and histomorphology. Damage to the liver was lowest after warm ischemia of 1 h. The results after cold storage were superior to those after normothermic perfusion (GOT: 3.2 +/- 0.3 and 2.6 +/- 0.2 U/g liver; cumulative bile production: 14.7 +/- 2.1 and 9.4 +/- 1 ml, respectively; P < 0.05). In contrast, we found substantial damage at the end of reperfusion in livers undergoing 3 h of warm ischemia under both preservation techniques with severe hepatocellular pyknoses and essentially altered nonparenchymal cells. The results suggest that pig livers undergoing 1 h of warm ischemia and cold storage for 3 h with HTK solution may lead to functioning after transplantation.
authors.affiliation: Abteilung für Allgemeine Chirurgie, Universität-GH-Essen.
authors.forename: A
authors.initials: A
authors.lastname: Hellinger
authors.forename: R
authors.initials: R
authors.lastname: Fiegen
authors.forename: R
authors.initials: R
authors.lastname: Lange
authors.forename: U
authors.initials: U
authors.lastname: Rauen
authors.forename: U
authors.initials: U
authors.lastname: Schmidt
authors.forename: H
authors.initials: H
authors.lastname: Hirche
authors.forename: S
authors.initials: S
authors.lastname: Kaiser
authors.forename: H
authors.initials: H
authors.lastname: de Groot
authors.forename: J
authors.initials: J
authors.lastname: Erhard
authors.forename: F W
authors.initials: FW
authors.lastname: Eigler
mesh_terms: D000410:Alanine Transaminase / Q000378:metabolism; D000818:Animals; D001219:Aspartate Aminotransferases / Q000378:metabolism; D001646:Bile / Q000378:metabolism; D001833:Body Temperature Regulation / Q000502:physiology; D003402:Creatine Kinase / Q000378:metabolism; D015925:Cryopreservation / Q000379:methods*; D005260:Female; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D008099:Liver / Q000473:pathology / Q000503:physiopathology; D008111:Liver Function Tests; D016031:Liver Transplantation / Q000473:pathology / Q000502:physiology*; D008297:Male; D009926:Organ Preservation / Q000379:methods*; D015427:Reperfusion Injury / Q000473:pathology / Q000503:physiopathology*; D013552:Swine; D014184:Transplantation, Homologous
chemical_list: D007770:L-Lactate Dehydrogenase; D001219:Aspartate Aminotransferases; D000410:Alanine Transaminase; D003402:Creatine Kinase