The sacB gene cannot be used as a counter-selectable marker in Pasteurella multocida.
The use of the Bacillus subtilis sacB gene as a counter-selectable marker was assessed in serogroup A and B strains of Pasteurella multocida. Expression ofsacB failed to render any of the strains sensitive to sucrose, indicating that the sacB gene can not be used as a positive selection system in P. multocida.
Jost
B H
BH
Department of Microbiology, Monash University, Clayton VIC, Australia.
Homchampa
P
P
Strugnell
R A
RA
Adler
B
B
D005819:Genetic Markers; D006602:Hexosyltransferases / Q000187:drug effects / Q000235:genetics* / Q000378:metabolism; D009154:Mutation; D016979:Pasteurella multocida / Q000235:genetics* / Q000378:metabolism; D012641:Selection, Genetic; D013395:Sucrose / Q000378:metabolism / Q000494:pharmacology; D014169:Transformation, Bacterial
D005819:Genetic Markers; D013395:Sucrose; D006602:Hexosyltransferases; C021513:levansucrase