title: Cloning and characterization of the lytB gene of Campylobacter jejuni.
abstract: LytB of Escherichia coli is an essential gene involved in penicillin tolerance and the stringent response. The lytB gene of Campylobacter jejuni was cloned and characterized. It could complement a temperature-sensitive E. coli lytB mutant. The C. jejuni lytB gene encodes a protein of 277 amino acids that has 34, 36 and 40% amino acid identity with the LytB proteins of E. coli, Haemophilus influenzae, and Synechocystis sp. PCC6803, respectively. The lytB gene is situated between the aroA gene and a gene that encodes ribosomal protein S1.
authors.affiliation: Department of Bacteriology, Faculty of Veterinary Medicine, Universiteit Utrecht, The Netherlands.
authors.forename: M M
authors.initials: MM
authors.lastname: Wösten
authors.forename: E E
authors.initials: EE
authors.lastname: Ishiguro
authors.forename: B A
authors.initials: BA
authors.lastname: van der Zeijst
mesh_terms: D001426:Bacterial Proteins / Q000737:chemistry / Q000235:genetics*; D016123:Campylobacter jejuni / Q000187:drug effects / Q000235:genetics*; D003001:Cloning, Molecular; D029968:Escherichia coli Proteins; D015964:Gene Expression Regulation, Bacterial; D005816:Genetic Complementation Test; D008969:Molecular Sequence Data; D010088:Oxidoreductases; D010403:Penicillin Resistance; D011509:Proteoglycans / Q000737:chemistry / Q000235:genetics*; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid
chemical_list: D001426:Bacterial Proteins; D029968:Escherichia coli Proteins; D011509:Proteoglycans; D010088:Oxidoreductases; C109866:ispH protein, E coli