RNA transcription and lysosomal activation during xenotransplant hyperacute rejection: inhibition of RNA polymerase.
In summary, our data indicates that AFB1 has no effect on LyAct during XTHAR. Although these results are from short-term incubation studies, they nevertheless suggest that inhibition of RNA transcription by AFB1 may be of no consequence in LyAct during XTHAR. Hence, our results appear to support the view that, in general, XTHAR recruits preformed components and may not require de novo synthesis of lysosomal proteins. Additional studies using long-term incubation (days to weeks) and other protein synthesis blockers are suggested to further elucidate the effects of protein synthesis inhibition of LyAct during XTHAR.
Reese
D W
DW
Howard University College of Medicine, Washington, DC, USA.
Odocha
O
O
D016604:Aflatoxin B1 / Q000494:pharmacology*; D000818:Animals; D001769:Blood; D002460:Cell Line; D003165:Complement System Proteins / Q000494:pharmacology; D012321:DNA-Directed RNA Polymerases / Q000037:antagonists & inhibitors*; D004791:Enzyme Inhibitors / Q000494:pharmacology; D006084:Graft Rejection / Q000378:metabolism*; D006801:Humans; D007668:Kidney / Q000166:cytology / Q000378:metabolism*; D008247:Lysosomes / Q000378:metabolism*; D018448:Models, Immunological; D011817:Rabbits; D013552:Swine; D014158:Transcription, Genetic / Q000187:drug effects; D014183:Transplantation, Heterologous / Q000276:immunology*
D004791:Enzyme Inhibitors; D003165:Complement System Proteins; D016604:Aflatoxin B1; D012321:DNA-Directed RNA Polymerases