[Chloride conduction of nasal fibroblasts in polyposis patients with cystic fibrosis and in patients without cystic fibrosis. Relevance for the ENT physician].
Cystic fibrosis (CF) is a complex systemic disease that has pathological alterations in the upper airways, including the recurrent formation of nasal polyps. Although the fibroblast is the predominant cell type in nasal stroma and nasal polyps, little is known about the electrophysiological properties of nasal fibroblasts. We investigated whether fibroblasts possess a cAMP-regulated chloride conductance which is impaired in patients with CF. Thus far the few studies concerning conductance in fibroblasts have been performed on skin fibroblasts using indirect methods and have yielded conflicting results. Therefore we studied chloride conductance in fused nasal fibroblasts by employing conventional microelectrodes. We have demonstrated that a cAMP-regulated chloride conductance is present in fibroblasts. However, this chloride conductance cannot be activated in fibroblasts from CF-patients. Thus, we present direct evidence that the impairment of the cAMP-regulated chloride conductance in CF is not confined to epithelial cells but also affects the fibroblast. We discuss how this conductance might modulate fibroblast proliferation to produce polyp formation.
Weber
A
A
Zentrum der Hals-Nasen-Ohrenheilkunde, Johann-Wolfgang-Goethe-Universität, Frankfurt am Main.
Schröder
U H
UH
May
A
A
von Ilberg
C
C
Frömter
E
E
D002648:Child; D002675:Child, Preschool; D018118:Chloride Channels / Q000502:physiology*; D002712:Chlorides / Q000378:metabolism*; D000242:Cyclic AMP / Q000502:physiology; D003550:Cystic Fibrosis / Q000503:physiopathology*; D005260:Female; D005347:Fibroblasts / Q000502:physiology; D006801:Humans; D008297:Male; D008564:Membrane Potentials / Q000502:physiology; D009298:Nasal Polyps / Q000503:physiopathology*; D009669:Nose Neoplasms / Q000503:physiopathology*
D018118:Chloride Channels; D002712:Chlorides; D000242:Cyclic AMP