Previous heat shock treatment inhibits Mayaro virus replication in human lung adenocarcinoma (A549) cells.
Human lung adenocarcinoma cells (A549) were submitted to mild or severe heat shock (42 degrees C or 44 degrees C) for 1 h, while another group of cells was double-heat-shocked (submitted to 42 degrees C for 1 h, returned to 37 degrees C for 3 h, then exposed to 44 degrees C for 1 h). After each heat treatment, the cells were infected with Mayaro virus for 24 h and incubated at 37 degrees C. The results showed that the double-heat-shocked thermotolerant cells exhibited a 10(4)-fold virus titre inhibition, despite the recovery of protein synthesis and original morphology 24 h post-infection. In contrast, cells submitted to mild or severe heat shock exhibited weaker inhibition of Mayaro virus titre (10(2)-fold). The mildly heat-shocked cells also presented a full recovery in protein synthesis, which was not observed in severely heat-shocked cells. These results indicate that exposure of A549 cells to a mild or to a double heat shock treatment before Mayaro virus infection induces an antiviral state.
Virgilio
P L
PL
Instituto de Biofisica Carlos Chagas Filho, Universidade Federal do Rio de Janeiro, Brazil.
Godinho-Netto
M C
MC
Carvalho Mda
G
G
D000230:Adenocarcinoma; D000524:Alphavirus / Q000254:growth & development / Q000502:physiology*; D000818:Animals; D002460:Cell Line; D006224:Cricetinae; D018869:Heat-Shock Response / Q000502:physiology*; D006801:Humans; D008175:Lung Neoplasms; D014176:Protein Biosynthesis; D013696:Temperature; D014407:Tumor Cells, Cultured; D014779:Virus Replication