The irradiation of hepatocytes with He-Ne laser causes an increase of cytosolic free calcium concentration and an increase of cell membrane potential, correlated with it, both increases taking place in an oscillatory manner.
Isolated hepatocytes were irradiated with Helium-Neon laser (fluence: 0.24 Joules x cm-2, fluence rate: 12 mW x cm-2) and changes of both cytosolic free Ca2+ concentration and cell membrane potential were checked by measuring fura-2 and bis-oxonol fluorescence respectively. Irradiation resulted in an enhancement in cytosolic free Ca2+ concentration that requires the presence of Ca2+ in the phase outside hepatocytes; consistently an increase in cell membrane potential was measured correlated with it. Interestingly, the rate of increase of both cytosolic free Ca2+ concentration and cell membrane potential shows special time dependent features similar to those peculiar of oscillatory processes.
Vacca
R A
RA
Centro Studi Mitocondri e Metabolismo Energetico, C.N.R., Bari, Italy.
Moro
L
L
Petragallo
V A
VA
Greco
M
M
Fontana
F
F
Passarella
S
S
D000818:Animals; D001683:Biological Clocks / Q000528:radiation effects*; D002118:Calcium / Q000378:metabolism* / Q000502:physiology / Q000528:radiation effects*; D002462:Cell Membrane / Q000528:radiation effects; D003600:Cytosol / Q000378:metabolism* / Q000502:physiology / Q000528:radiation effects*; D006371:Helium; D007834:Lasers; D008027:Light; D008099:Liver / Q000166:cytology / Q000502:physiology* / Q000528:radiation effects*; D008297:Male; D008564:Membrane Potentials / Q000528:radiation effects; D009356:Neon; D051381:Rats; D017208:Rats, Wistar
D006371:Helium; D009356:Neon; D002118:Calcium