title: Expression of nm23-H1 in human meningioma cells.
abstract: The expression of nm23-H1 has been demonstrated to be highly correlated with the metastatic potential of various tumors. In the present investigation, meningiomas of different pathological grades were used to study on their nm23-H1 expression. Immunohistochemistry showed that nm23-H1 was expressed mainly in the cytoplasm especially in the perinuclear region in explants under short-term culture. Western-blotting demonstrated the specific expression of nm23 protein in all tumor samples. The expression was also found to be sex-dependent on tumor progression in female, but not in male patients. RT-PCR results confirmed nm23-H1 expression was higher in benign tumors than in their normal counterpart. Our observations thus suggest that nm23-H1 may play an important role in the progression of meningiomas in female patients.
authors.affiliation: Department of Anatomy, Chinese University of Hong Kong, NT, Hong Kong.
authors.forename: S W
authors.initials: SW
authors.lastname: Wang
authors.forename: H F
authors.initials: HF
authors.lastname: Yam
authors.forename: L
authors.initials: L
authors.lastname: Yang
authors.forename: H K
authors.initials: HK
authors.lastname: Ng
authors.forename: S L
authors.initials: SL
authors.lastname: Wang
authors.forename: B Q
authors.initials: BQ
authors.lastname: Wu
authors.forename: Z H
authors.initials: ZH
authors.lastname: Wang
authors.forename: S B
authors.initials: SB
authors.lastname: Chew-Cheng
authors.forename: E C
authors.initials: EC
authors.lastname: Chew
mesh_terms: D000328:Adult; D000368:Aged; D016543:Central Nervous System Neoplasms / Q000235:genetics / Q000378:metabolism*; D005260:Female; D015870:Gene Expression; D006801:Humans; D008297:Male; D008579:Meningioma / Q000235:genetics / Q000378:metabolism*; D008875:Middle Aged; D020559:Monomeric GTP-Binding Proteins; D054778:NM23 Nucleoside Diphosphate Kinases; D009363:Neoplasm Proteins / Q000378:metabolism*; D009701:Nucleoside-Diphosphate Kinase; D012333:RNA, Messenger / Q000235:genetics; D012334:RNA, Neoplasm / Q000235:genetics; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism*
chemical_list: D054778:NM23 Nucleoside Diphosphate Kinases; D009363:Neoplasm Proteins; D012333:RNA, Messenger; D012334:RNA, Neoplasm; D014157:Transcription Factors; C517938:NME1 protein, human; D009701:Nucleoside-Diphosphate Kinase; D020559:Monomeric GTP-Binding Proteins