title: Discrete start sites for DNA synthesis in the yeast ARS1 origin.
abstract: Sites of DNA synthesis initiation have been detected at the nucleotide level in a yeast origin of bidirectional replication with the use of replication initiation point mapping. The ARS1 origin of Saccharomyces cerevisiae showed a transition from discontinuous to continuous DNA synthesis in an 18-base pair region (nucleotides 828 to 845) from within element B1 toward B2, adjacent to the binding site for the origin recognition complex, the putative initiator protein.
authors.affiliation: Department of Molecular Biology, Cell Biology and Biochemistry, Division of Biology and Medicine, Brown University, Providence, RI 02912, USA.
authors.forename: A K
authors.initials: AK
authors.lastname: Bielinsky
authors.forename: S A
authors.initials: SA
authors.lastname: Gerbi
mesh_terms: D001482:Base Composition; D001483:Base Sequence; D001665:Binding Sites; D004265:DNA Helicases / Q000378:metabolism; D017931:DNA Primers; D004261:DNA Replication; D004271:DNA, Fungal / Q000096:biosynthesis*; D004268:DNA-Binding Proteins; D008969:Molecular Sequence Data; D018741:Replication Origin; D012441:Saccharomyces cerevisiae / Q000378:metabolism*; D015534:Trans-Activators / Q000378:metabolism
chemical_list: D017931:DNA Primers; D004271:DNA, Fungal; D004268:DNA-Binding Proteins; D015534:Trans-Activators; C045264:replication initiator protein; D004265:DNA Helicases