title: Helicobacter pylori populations in Peruvian patients.
abstract: Helicobacter pylori is an extremely diverse species. The characterization of strains isolated from individual patients should give insights into colonization and disease mechanisms and bacterial evolution. We studied H. pylori isolates from patients in the Japanese-Peruvian Polyclinic in Lima, Peru, by determining metronidazole susceptibility or resistance and by random amplified polymorphic DNA (RAPD) fingerprinting (a measure of overall genotype). Strains isolated from several biopsy specimens from each of 24 patients were studied. Both metronidazole-susceptible and -resistant strains were isolated from 13 patients, whereas strains of more than one RAPD type were isolated from only seven patients. We propose that the homogeneity in RAPD fingerprints for strains isolated from most persons reflects selection for particular H. pylori genotypes during chronic infection in individual hosts and the human diversity in traits that are important to this pathogen. Carriage of related metronidazole-resistant and -susceptible strains could reflect frequent metronidazole use in Peru and alternating selection for resistant and susceptible phenotypes during and after metronidazole therapy.
authors.affiliation: Department of Molecular Microbiology, Washington University Medical School, St. Louis, Missouri 63110, USA. BERG@borcim.wustl@edu
authors.forename: D E
authors.initials: DE
authors.lastname: Berg
authors.forename: R H
authors.initials: RH
authors.lastname: Gilman
authors.forename: J
authors.initials: J
authors.lastname: Lelwala-Guruge
authors.forename: K
authors.initials: K
authors.lastname: Srivastava
authors.forename: Y
authors.initials: Y
authors.lastname: Valdez
authors.forename: J
authors.initials: J
authors.lastname: Watanabe
authors.forename: J
authors.initials: J
authors.lastname: Miyagi
authors.forename: N S
authors.initials: NS
authors.lastname: Akopyants
authors.forename: A
authors.initials: A
authors.lastname: Ramirez-Ramos
authors.forename: T H
authors.initials: TH
authors.lastname: Yoshiwara
authors.forename: S
authors.initials: S
authors.lastname: Recavarren
authors.forename: R
authors.initials: R
authors.lastname: Leon-Barua
mesh_terms: D000328:Adult; D000368:Aged; D000900:Anti-Bacterial Agents / Q000494:pharmacology; D016172:DNA Fingerprinting; D004352:Drug Resistance, Microbial / Q000235:genetics; D005260:Female; D016481:Helicobacter Infections / Q000453:epidemiology / Q000382:microbiology*; D016480:Helicobacter pylori / Q000187:drug effects / Q000235:genetics / Q000302:isolation & purification; D006801:Humans; D008297:Male; D008795:Metronidazole / Q000494:pharmacology; D008875:Middle Aged; D010568:Peru / Q000453:epidemiology; D019105:Random Amplified Polymorphic DNA Technique
chemical_list: D000900:Anti-Bacterial Agents; D008795:Metronidazole