title: Serum oxalate microassay using chemiluminescence detection.
abstract: Determination of serum oxalate concentration is important for the diagnosis and monitoring of hyperoxalurias, and extends to patients with all types of renal disease. Approximately 5 to 10 ml of blood is required for each test by conventional methods, and the test is not adapted for use in children. We developed a highly sensitive method that limits the volume of blood required for the test. This new and sensitive tool to detect H2O2 can be successfully substituted for the conventional, and expensive, colorimetric reaction to accurately analyze oxalate concentration.
authors.affiliation: Service de Biochimie, Pharmacotoxicologie et Analyse des Traces, Hôpital Edouard Herriot, Lyon, France.
authors.forename: J M
authors.initials: JM
authors.lastname: Gaulier
authors.forename: P
authors.initials: P
authors.lastname: Cochat
authors.forename: G
authors.initials: G
authors.lastname: Lardet
authors.forename: J J
authors.initials: JJ
authors.lastname: Vallon
mesh_terms: D000328:Adult; D002624:Chemistry, Clinical / Q000379:methods*; D003124:Colorimetry; D005260:Female; D006801:Humans; D006861:Hydrogen Peroxide / Q000032:analysis; D008163:Luminescent Measurements; D008165:Luminol; D008297:Male; D008875:Middle Aged; D010070:Oxalates / Q000032:analysis* / Q000097:blood*; D010084:Oxidation-Reduction; D017785:Photons; D012680:Sensitivity and Specificity
chemical_list: D010070:Oxalates; D008165:Luminol; D006861:Hydrogen Peroxide