Cytosolic aspartate aminotransferase gene is a member of the glucose-regulated protein gene family in adipocytes.
Stress controls the expression of a cohort of genes. Among these, the glucose-regulated protein (GRP) genes are specifically activated by glucose deprivation, reducing agents, glycosylation block, intracellular calcium or ex vivo incubations of tissues or cells. We demonstrate that these stimuli induce the expression of the cytosolic aspartate aminotransferase gene in adipocytes by a process involving the region of the promoter between -2405 and -26 bp. Therefore this transaminase is a new member of the GRP family.
Plee-Gautier
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Centre de Recherche sur l'Endocrinologie Moléculaire et le Développement, C.N.R.S., 9, rue Jules Hetzel, 92190 Meudon, France.
Grimal
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Aggerbeck
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Barouki
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Forest
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D016475:3T3 Cells; D017667:Adipocytes / Q000737:chemistry / Q000201:enzymology*; D000818:Animals; D001219:Aspartate Aminotransferases / Q000235:genetics*; D015152:Blotting, Northern; D000001:Calcimycin / Q000494:pharmacology; D015500:Chloramphenicol O-Acetyltransferase / Q000032:analysis; D003600:Cytosol / Q000201:enzymology; D015971:Gene Expression Regulation, Enzymologic; D017930:Genes, Reporter; D005947:Glucose / Q000378:metabolism; D018840:HSP70 Heat-Shock Proteins / Q000235:genetics*; D006358:Hot Temperature; D008565:Membrane Proteins / Q000235:genetics*; D008623:Mercaptoethanol / Q000494:pharmacology; D051379:Mice; D011401:Promoter Regions, Genetic; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D013312:Stress, Physiological; D014162:Transfection; D014415:Tunicamycin / Q000494:pharmacology
D018840:HSP70 Heat-Shock Proteins; D008565:Membrane Proteins; D012333:RNA, Messenger; C018551:glucose-regulated proteins; D014415:Tunicamycin; D000001:Calcimycin; D008623:Mercaptoethanol; D015500:Chloramphenicol O-Acetyltransferase; D001219:Aspartate Aminotransferases; D005947:Glucose