title: Detection of immunoglobulin G (IgG) and IgM antibodies to Toxoplasma gondii: evaluation of four commercial immunoassay systems.
abstract: A comparative evaluation of the following commercial immunoassays for the determination of antibodies to Toxoplasma gondii was performed: Behring Diagnostics OPUS Toxo G and Toxo M, Abbott Diagnostics IMX Toxo-IgG 2.0 and Toxo-IgM, Sanofi Diagnostics Pasteur Platelia Toxo IgG and Toxo IgM, and bioMérieux Vitek VIDAS Toxo IgG and IgM. Of 676 specimens that were tested for Toxoplasma-specific immunoglobulin G (IgG) antibodies, 26% were reactive by all methods while 8% displayed some discrepancy. Of 718 specimens that were tested for Toxoplasma-specific IgM antibodies, 3% were reactive by all methods while 10% displayed some discrepancy. Analysis of discrepant specimens revealed performance shortcomings with all IgM-specific assays. The impact of such shortcomings is magnified in a population with a low prevalence of toxoplasmosis.
authors.affiliation: Department of Laboratory Medicine, University of Washington, Seattle, USA.
authors.forename: W T
authors.initials: WT
authors.lastname: Hofgärtner
authors.forename: S R
authors.initials: SR
authors.lastname: Swanzy
authors.forename: R M
authors.initials: RM
authors.lastname: Bacina
authors.forename: J
authors.initials: J
authors.lastname: Condon
authors.forename: M
authors.initials: M
authors.lastname: Gupta
authors.forename: P E
authors.initials: PE
authors.lastname: Matlock
authors.forename: D L
authors.initials: DL
authors.lastname: Bergeron
authors.forename: J J
authors.initials: JJ
authors.lastname: Plorde
authors.forename: T R
authors.initials: TR
authors.lastname: Fritsche
mesh_terms: D000818:Animals; D000913:Antibodies, Protozoan / Q000097:blood*; D005069:Evaluation Studies as Topic; D006801:Humans; D007118:Immunoassay / Q000379:methods* / Q000706:statistics & numerical data; D007074:Immunoglobulin G / Q000097:blood; D007075:Immunoglobulin M / Q000097:blood; D010274:Parasitology / Q000379:methods* / Q000706:statistics & numerical data; D011237:Predictive Value of Tests; D012680:Sensitivity and Specificity; D014122:Toxoplasma / Q000276:immunology*; D014123:Toxoplasmosis / Q000175:diagnosis* / Q000276:immunology
chemical_list: D000913:Antibodies, Protozoan; D007074:Immunoglobulin G; D007075:Immunoglobulin M