Autoantibody formation against a 190-kDa antigen of the desmosomal plaque in pemphigus foliaceus.
We report changes in the antigen recognition pattern of sera from two pemphigus foliaceus patients with a long-term follow-up. The patients' sera were analysed by immunoblotting using different antigenic sources: cultured human keratinocytes, bovine tongue epithelium and a recombinant protein corresponding to the C-terminal end of the 230-kDa bullous pemphigoid antigen. While initial serum samples reacted exclusively with the 160-kDa desmoglein 1, the later sera reacted both with desmoglein 1 and a 190-kDa antigen immunolocalized to the desmosomal plaque, previously demonstrated to be recognized by sera of some patients with paraneoplastic pemphigus. IgG subclass analysis further showed that antidesmoglein 1 antibodies were of IgG1 and/or IgG4 subclasses, while anti-190-kDa antibodies were IgG3. The patients were free of malignancy.
Ghohestani
R
R
Clinique Dermatologique, Hôpital Edouard Herriot, Lyon.
Joly
P
P
Gilbert
D
D
Nicolas
J F
JF
Thomine
E
E
Cozzani
E
E
Lauret
P H
PH
Claudy
A
A
Tron
F
F
D000368:Aged; D001323:Autoantibodies / Q000096:biosynthesis*; D001324:Autoantigens / Q000276:immunology*; D003896:Desmosomes / Q000276:immunology*; D005260:Female; D019084:Fluorescent Antibody Technique, Indirect; D005500:Follow-Up Studies; D006801:Humans; D015151:Immunoblotting; D008297:Male; D016253:Microscopy, Immunoelectron; D008875:Middle Aged; D010392:Pemphigus / Q000276:immunology* / Q000473:pathology
D001323:Autoantibodies; D001324:Autoantigens