title: Mutations of the MEN1 tumor suppressor gene in pituitary tumors.
abstract: Although pituitary adenomas are monoclonal proliferations, somatic mutations involving genes that govern cell proliferation or hormone production have been difficult to identify. The genetic etiology of most pituitary tumors, therefore, remains unknown. Pituitary adenomas can develop sporadically or as a part of multiple endocrine neoplasia type 1 (MEN1). Recently, the gene responsible for MEN1 was cloned. To elucidate the potential etiological role of the MEN1 gene in pituitary tumorigenesis, 39 sporadic pituitary adenomas from 38 patients and 1 pituitary adenoma from a familial MEN1 patient were examined for MEN1 gene mutations and allelic deletions. Four of 39 sporadic pituitary adenomas showed a deletion of one copy of the MEN1 gene, and a specific MEN1 gene mutation in the remaining gene copy was detected in 2 of these tumors. The corresponding germ-line sequence was normal in all sporadic cases. A specific MEN1 mutation was detected in a pituitary adenoma and corresponding germ-line DNA in a patient with familial MEN1. An allelic deletion of the remaining copy of the MEN1 gene was also found in the patient's tumor. Genetic alterations of the MEN1 gene represent a candidate pathogenetic mechanism of pituitary tumorigenesis. The data suggest that somatic MEN1 gene mutations and deletions play a causative role in the development of a subgroup of sporadic pituitary adenomas.
authors.affiliation: Laboratory of Pathology, National Cancer Institute, NIH, Bethesda, Maryland 20892, USA.
authors.forename: Z
authors.initials: Z
authors.lastname: Zhuang
authors.forename: S Z
authors.initials: SZ
authors.lastname: Ezzat
authors.forename: A O
authors.initials: AO
authors.lastname: Vortmeyer
authors.forename: R
authors.initials: R
authors.lastname: Weil
authors.forename: E H
authors.initials: EH
authors.lastname: Oldfield
authors.forename: W S
authors.initials: WS
authors.lastname: Park
authors.forename: S
authors.initials: S
authors.lastname: Pack
authors.forename: S
authors.initials: S
authors.lastname: Huang
authors.forename: S K
authors.initials: SK
authors.lastname: Agarwal
authors.forename: S C
authors.initials: SC
authors.lastname: Guru
authors.forename: P
authors.initials: P
authors.lastname: Manickam
authors.forename: L V
authors.initials: LV
authors.lastname: Debelenko
authors.forename: M B
authors.initials: MB
authors.lastname: Kester
authors.forename: S E
authors.initials: SE
authors.lastname: Olufemi
authors.forename: C
authors.initials: C
authors.lastname: Heppner
authors.forename: J S
authors.initials: JS
authors.lastname: Crabtree
authors.forename: A L
authors.initials: AL
authors.lastname: Burns
authors.forename: A M
authors.initials: AM
authors.lastname: Spiegel
authors.forename: S J
authors.initials: SJ
authors.lastname: Marx
authors.forename: S C
authors.initials: SC
authors.lastname: Chandrasekharappa
authors.forename: F S
authors.initials: FS
authors.lastname: Collins
authors.forename: M R
authors.initials: MR
authors.lastname: Emmert-Buck
authors.forename: L A
authors.initials: LA
authors.lastname: Liotta
authors.forename: S L
authors.initials: SL
authors.lastname: Asa
authors.forename: I A
authors.initials: IA
authors.lastname: Lubensky
mesh_terms: D000236:Adenoma / Q000235:genetics*; D000328:Adult; D000368:Aged; D002675:Child, Preschool; D005260:Female; D016147:Genes, Tumor Suppressor; D006801:Humans; D008297:Male; D008875:Middle Aged; D018761:Multiple Endocrine Neoplasia Type 1 / Q000235:genetics*; D009154:Mutation; D010911:Pituitary Neoplasms / Q000235:genetics*