Effects of insulin and insulin-like growth factors I and II on the growth of equine fetal and neonatal chondrocytes.
The effects of insulin and insulin-like growth factors (IGFs) I and II on fetal and foal chondrocytes were investigated in vitro. Chondrocytes from the lateral trochlear ridge of the distal femur were obtained from 2 fetuses (280 and 320 days gestation) and one 4-day-old foal and cultured. Membrane proteins consistent with type 1 and type 2 IGF receptors were demonstrated by radioligand cross linking and equilibrium binding analysis. It was demonstrated that both IGF-I and IGF-II acted as mitogens for isolated equine chondrocytes when present as the sole mitogenic factor in monolayer culture. It was further shown that whereas insulin was able to promote the survival and expansion of cell populations of chondrocytes in culture there was significantly reduced mitogenic stimulation compared to the IGFs. These results suggest that the role of insulin in growth cartilage may be to promote chondrocyte survival, or to suppress differentiation/apoptosis. This supports the hypothesis that relative hyperinsulinaemia may be a contributory factor to equine dyschondroplasia (osteochondrosis). Understanding of contributory, and possibly triggering factors such as this may allow the development of modified methods of husbandry which minimise the risk of disease in populations with a known predisposition.
Henson
F M
FM
Department of Clinical Veterinary Medicine, University of Cambridge, UK.
Davenport
C
C
Butler
L
L
Moran
I
I
Shingleton
W D
WD
Jeffcott
L B
LB
Schofield
P N
PN
D000818:Animals; D000831:Animals, Newborn / Q000254:growth & development* / Q000502:physiology; D017209:Apoptosis / Q000187:drug effects / Q000502:physiology; D002358:Cartilage, Articular / Q000166:cytology* / Q000196:embryology / Q000254:growth & development; D015984:Causality; D002453:Cell Cycle; D002454:Cell Differentiation / Q000187:drug effects / Q000502:physiology; D002455:Cell Division / Q000187:drug effects / Q000502:physiology; D002462:Cell Membrane / Q000737:chemistry; D002478:Cells, Cultured; D004247:DNA / Q000096:biosynthesis; D005260:Female; D005269:Femur / Q000166:cytology / Q000196:embryology / Q000254:growth & development; D006734:Horse Diseases / Q000453:epidemiology; D006736:Horses / Q000196:embryology* / Q000254:growth & development* / Q000502:physiology; D007328:Insulin / Q000494:pharmacology*; D007334:Insulin-Like Growth Factor I / Q000494:pharmacology*; D007335:Insulin-Like Growth Factor II / Q000494:pharmacology*; D010009:Osteochondrodysplasias / Q000453:epidemiology / Q000662:veterinary; D011247:Pregnancy; D017451:Receptors, Somatomedin / Q000032:analysis; D012307:Risk Factors; D013936:Thymidine / Q000378:metabolism
D007328:Insulin; D017451:Receptors, Somatomedin; D007334:Insulin-Like Growth Factor I; D007335:Insulin-Like Growth Factor II; D004247:DNA; D013936:Thymidine