Speciation of aluminium in tea infusions studied by size exclusion chromatography with detection by post-column reaction.
Aluminium-organic species in tea infusions were studied by size exclusion chromatography, using a Superose 12 HR column, a mobile phase of 0.12 mol l-1 tris(hydroxymethyl)aminomethane adjusted to pH 5.5 and detection by UV-visible spectrophotometry. Aluminium was detected after post-column reaction with pyrocatechol violet at 585 nm and the organic material was detected at 280 nm. Teas of different origin were analyzed; the results indicate that aluminium is bound to the same relatively narrow size-range of large organic molecules in the tea infusions, irrespective of the origin of the tea.
Flaten
A K
AK
Department of Chemistry, University of Oslo, Norway.
Lund
W
W
D000535:Aluminum / Q000032:analysis* / Q000378:metabolism; D001557:Benzenesulfonates / Q000737:chemistry; D002853:Chromatography, Liquid / Q000379:methods*; D012680:Sensitivity and Specificity; D013053:Spectrophotometry / Q000295:instrumentation / Q000379:methods; D013054:Spectrophotometry, Atomic / Q000379:methods; D013662:Tea / Q000737:chemistry*; D014466:Ultraviolet Rays
D001557:Benzenesulfonates; D013662:Tea; C009134:pyrocatechol violet; D000535:Aluminum