title: X-ray fibre diffraction study of an elevated temperature structure of poly(dA).poly(dT).
abstract: A reversible conformational transition between two discrete double helical forms of poly(dA).poly(dT) has been put into evidence by X-ray fibre diffraction. We observed that the transition between the well known B' conformation and a new helical structure (B*) occurs at a relative humidity near 80%, when the temperature is raised above 30 degrees C. It appears that the B* conformation is not just a distorted B' form of poly(dA).poly(dT) but rather a stable (up to a least 70 degrees C) distinct double helical structure of that polynucleotide. Analysis of X-ray patterns allowed us to present the geometrical parameters of a molecular model of this new double helix. It consists of 11.4 nucleotide pairs per turn in a pitch length of about 36.7 A. The proposed high-temperature right handed helical structure of poly(dA).poly(dT) is a member of the B-DNA family since the duplex has C1'-exo furanoses in both antiparallel but geometrically identical sugar-phosphate strands. The present finding may shed light on interpretations of results obtained from premelting or nucleosome formation processes involving (dA.dT) tracts in synthetic or natural DNA polymers.
authors.affiliation: Laboratoire de Biophysique Moléculaire, Université H. Poincaré-Nancy 1, Vandoeuvre les Nancy, France.
authors.forename: S
authors.initials: S
authors.lastname: Premilat
authors.forename: G
authors.initials: G
authors.lastname: Albiser
mesh_terms: D001482:Base Composition; D005583:Fourier Analysis; D006358:Hot Temperature; D008958:Models, Molecular; D009690:Nucleic Acid Conformation; D011067:Poly dA-dT / Q000737:chemistry*; D013237:Stereoisomerism; D013696:Temperature; D014961:X-Ray Diffraction / Q000379:methods
chemical_list: D011067:Poly dA-dT