Regulation of mouse immuno-responses by a natural suppressor cell clone from bone marrow.
Natural suppressor (NS) activity is mediated by several kinds of cell lineages in bone marrow. We demonstrated that a NS cell, clone 7-31, was obtained after limiting dilution with bone marrow culture supplemented with WEHI supernatant. Clone 7-31, was capable of non-specific suppression of the generation of cytotoxic T lymphocytes without major histocompatibility complex restriction. Suppression of cytotoxic thymus-dependent lymphocyte (CTL) generation was also induced with a cell-free supernatant from the 7-31 cells. Interleukin-2 (IL-2) containing concanavallin-A-conditioned medium could not reverse the suppression. The supernatant did not inhibit the proliferation of IL-2-dependent CTLL-2 cells and rapidly growing tumour cells and fibroblasts. Thus, this bone marrow suppressor cell produces a soluble factor that inhibits the generation of CTL in vitro, and prolongs the acceptance of skin allografts in in vivo.
Yanagië
H
H
Department of Surgery, University of Tokyo, Japan.
Chen
Z
Z
Takeda
Y
Y
Sugiyama
H
H
Sekiguchi
M
M
Eriguchi
M
M
D000818:Animals; D000941:Antigens / Q000276:immunology; D001854:Bone Marrow Cells / Q000276:immunology*; D002999:Clone Cells / Q000187:drug effects / Q000276:immunology; D003601:Cytotoxicity Tests, Immunologic; D005347:Fibroblasts / Q000166:cytology / Q000276:immunology; D006085:Graft Survival / Q000276:immunology; D007165:Immunosuppression Therapy; D007376:Interleukin-2 / Q000494:pharmacology; D007694:Killer Cells, Natural / Q000166:cytology / Q000276:immunology; D008213:Lymphocyte Activation / Q000187:drug effects / Q000276:immunology*; D051379:Mice; D008809:Mice, Inbred C3H; D008810:Mice, Inbred C57BL; D008811:Mice, Inbred DBA; D008934:Mitogens / Q000494:pharmacology; D016038:Skin Transplantation / Q000276:immunology; D013602:T-Lymphocytes, Cytotoxic / Q000166:cytology / Q000276:immunology; D014184:Transplantation, Homologous; D014407:Tumor Cells, Cultured / Q000276:immunology
D000941:Antigens; D007376:Interleukin-2; D008934:Mitogens