Different responses of apolipoprotein A-I, A-IV, and B gene expression during intestinal adaptation to a massive small bowel resection in rats.
Gene expression of apolipoproteins (apo) A-I, A-IV, and B, the predominant protein components of chylomicrons, was investigated in the residual ileum after a massive small bowel resection in rats. A Northern blot analysis showed that the apo A-IV mRNA level, but not the apo A-I and B mRNA levels, in the ileum was significantly higher in the resected rats than in the sham-operated rats 24 h and 2 wk post-surgery. RT-PCR coupled with a primer extension assay revealed that the apo B-48 mRNA/apo B-100 mRNA ratio, i.e., apo B mRNA editing, in the ileum was unchanged by the resection. It is thus concluded that, among the major intestinal apolipoproteins, apo A-IV is the only one whose gene expression is influenced by loss of the proximal intestine.
Sonoyama
K
K
Department of Bioscience and Chemistry, Faculty of Agriculture, Hokkaido University, Sapporo, Japan.
Aoyama
Y
Y
D000222:Adaptation, Physiological / Q000502:physiology*; D000818:Animals; D016632:Apolipoprotein A-I / Q000096:biosynthesis / Q000235:genetics; D016633:Apolipoprotein A-II / Q000096:biosynthesis / Q000235:genetics; D001053:Apolipoproteins / Q000096:biosynthesis* / Q000235:genetics; D001055:Apolipoproteins B / Q000096:biosynthesis / Q000235:genetics; D015152:Blotting, Northern; D018076:DNA, Complementary / Q000378:metabolism; D015870:Gene Expression; D007082:Ileum / Q000378:metabolism* / Q000502:physiology* / Q000601:surgery; D008297:Male; D016133:Polymerase Chain Reaction; D012313:RNA / Q000378:metabolism; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar
D016632:Apolipoprotein A-I; D016633:Apolipoprotein A-II; D001053:Apolipoproteins; D001055:Apolipoproteins B; D018076:DNA, Complementary; D012333:RNA, Messenger; D012313:RNA