Chromatography on cells and biomolecular assemblies.
Red cells, biomembrane vesicles, proteoliposomes and liposomes non-covalently immobilized in gel particles or beads have been used as stationary phases for biomembrane affinity analyses and ion-exchange chromatographic separation. Lipid monolayers coupled to silica beads have been utilized for membrane protein purification in detergent solution and plant cell walls for group separation of macromolecules according to size and charge. Further methodological studies are essential to implement general practical application.
Lundqvist
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Department of Biochemistry, Biomedical Center, Uppsala University, Sweden.
Lundahl
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D000595:Amino Acid Sequence; D001704:Biopolymers / Q000737:chemistry*; D002473:Cell Wall / Q000737:chemistry; D002853:Chromatography, Liquid / Q000379:methods*; D004910:Erythrocyte Membrane / Q000737:chemistry; D004912:Erythrocytes / Q000737:chemistry; D006801:Humans; D008051:Lipid Bilayers / Q000737:chemistry; D008081:Liposomes / Q000737:chemistry; D008567:Membranes, Artificial; D008863:Microspheres; D010455:Peptides / Q000737:chemistry; D059828:Plant Cells; D010944:Plants / Q000648:ultrastructure
D001704:Biopolymers; D008051:Lipid Bilayers; D008081:Liposomes; D008567:Membranes, Artificial; D010455:Peptides