title: [Chromosomal aberrations induced in human spermatozoa by mitomycin C].
abstract: Human spermatozoa were exposed to Mitomycin C (MM() at the concentrations of 7.5, 15, 3O micrograms/ml for 1 hour respectively before interspecific in vitro fertilization with zona-free hamster ova. The first cleavage metaphases was analyzed to investigate MMC-induced effects on human spermatozoa. The results showed a distinct dose-response relationship in the induction of chromosomal aberrations. The chromatid aberrations were the predominant types in MMC-induced changes. Some chromosome-type aberrations were also observed. This suggested that the induced effects of MMC on human spermatozoa were some different from that of ultraviolet light-like clastogens on somatic cells. 36.89% of MMC-induced aberrations were rejoining-type. This indicated that the DNA repair systems present in golden hamster oocytes were effective in repairing MMC-induced DNA lesions in human spermatozoa.
authors.affiliation: Shantou University Medical College.
authors.forename: T
authors.initials: T
authors.lastname: Huang
authors.forename: X
authors.initials: X
authors.lastname: Liu
authors.forename: M
authors.initials: M
authors.lastname: Cai
authors.forename: J
authors.initials: J
authors.lastname: Huang
mesh_terms: D000818:Animals; D000903:Antibiotics, Antineoplastic / Q000633:toxicity*; D002869:Chromosome Aberrations; D006224:Cricetinae; D004305:Dose-Response Relationship, Drug; D005260:Female; D006801:Humans; D008297:Male; D016685:Mitomycin / Q000633:toxicity*; D013094:Spermatozoa / Q000187:drug effects* / Q000648:ultrastructure
chemical_list: D000903:Antibiotics, Antineoplastic; D016685:Mitomycin