Identification of 5' flanking sequences that affect human pancreatic cholesterol esterase gene expression.
Pancreatic cholesterol esterase (CEL) is shown to play a significant role in cholesterol metabolism. As the hydrolytic property of CEL is important for transport of lipid esters, the extent of its expression is an important factor in the metabolism of lipids. Therefore, to identify the elements that modulate the transcription of its mRNA, we obtained several cosmid clones carrying the CEL gene. From one of these cosmid clones a 6.5-kb SmaI fragment that hybridizes to the 5' untranslated region of CEL cDNA was subcloned. Primer extension and S1 protection assays revealed that the 5' untranslated region is relatively short (only 20 nucleotides long). An analysis of the 5' flanking sequence revealed typical TATA and CCAAT boxes that impart tissue specificity. Further, consensus sequences of several cis elements described earlier could also be detected in this region. To identify the promoter sequences, various deletion constructs of the 5' region were made using polymerase chain reaction. These constructs were subcloned into a bacterial plasmid vector carrying chloramphenicol acetyltransferase (CAT) as the reporter gene and transfected into HepG-2 cells. CAT activity in the cell homogenate of the transfected cells was measured 48 h after transfection. Results showed that the promoter activity of human pancreatic CEL mRNA is in a large segment of 5' flanking sequences spanning the -10 and -930 nucleotides of its gene.
Kumar
V B
VB
Geriatric Research Education and Clinical Center, St. Louis University Health Sciences Center, MO, USA. kumar@slu.edu
Sasser
T
T
Mandava
J B
JB
al Sadi
H
H
Spilburg
C
C
D001482:Base Composition; D001483:Base Sequence; D003001:Cloning, Molecular; D005786:Gene Expression Regulation; D006801:Humans; D008969:Molecular Sequence Data; D010179:Pancreas / Q000201:enzymology*; D011401:Promoter Regions, Genetic; D012333:RNA, Messenger / Q000235:genetics; D012045:Regulatory Sequences, Nucleic Acid / Q000502:physiology*; D002787:Sterol Esterase / Q000235:genetics*
D012333:RNA, Messenger; D002787:Sterol Esterase