title: Acid phosphatase activity in Perkinsus marinus, the protistan parasite of the American oyster, Crassostrea virginica.
abstract: The effect of temperature (4, 12, 20, and 28 C) and osmolality (400, 570, and 840 mOsm/kg) on extracellular acid phosphatase (AP) secretion in vitro, and ultrastructural localization of AP activity in the parasite were investigated. The extracellular AP secretion by Perkinsus marinus was cell density dependent (P < 0.001). Increasing culture temperatures resulted in increased P. marinus proliferation concomitant with AP secretion (P < 0.0001). AP secretion was similar in P. marinus cultured at 400 and 570 mOsm/kg media, but higher than P. marinus cultured at 870 mOsm/kg media. Results of the ultrastructural study revealed that intense AP activity was in the nucleus of the parasite. Based on its distribution in the nucleus, AP may be playing a role in events leading to cell cycle regulation.
authors.affiliation: Virginia Institute of Marine Science, School of Marine Science, College of William & Mary, Gloucester Point 23062, USA.
authors.forename: A K
authors.initials: AK
authors.lastname: Volety
authors.forename: F L
authors.initials: FL
authors.lastname: Chu
mesh_terms: D000135:Acid Phosphatase / Q000378:metabolism* / Q000648:ultrastructure; D000818:Animals; D016782:Apicomplexa / Q000201:enzymology* / Q000254:growth & development / Q000502:physiology / Q000648:ultrastructure; D002455:Cell Division; D003470:Culture Media / Q000378:metabolism; D004789:Enzyme Activation; D007854:Lead / Q000378:metabolism; D009994:Osmolar Concentration; D010125:Ostreidae / Q000469:parasitology*; D013696:Temperature
chemical_list: D003470:Culture Media; D007854:Lead; C036682:lead phosphate; D000135:Acid Phosphatase