title: Susceptibility of Helicobacter pylori to the bactericidal activity of human serum.
abstract: BACKGROUND
  Human serum represents an important barrier to the entry of most mucosal organisms into tissues and to the systemic circulation. If at all present, Helicobacter pylori within gastric tissue is rare, and bacteremia for this organism has been described only once.
  
  
  METHODS
  To assess the susceptibility of H. pylori to the bactericidal activity present in normal human serum (NHS), we examined 13 H. pylori isolates. To assess the contributions of the classical and alternative complement pathways to killing, we added either C2-deficient or factor B-deficient serum, respectively, to heat-inactivated NHS. Also we assessed the ability of the strains to bind 125I-C3.
  
  
  RESULTS
  After incubation for 60 minutes at 37 degrees C, all 13 H. pylori strains were killed by NHS; heating to 56 degrees C for 30 minutes ablated killing, indicating complement dependence for this phenomenon. In the absence of an antibody source, there was no killing when either an alternative or classical complement pathway source was used. Adding B-deficient serum to heat-inactivated normal human serum did not restore killing, but adding C2-deficient serum permitted partial killing. All of the 13 strains bound 125I-C3. Although the kinetics varied from strain to strain, C3 bound was significantly correlated (r = 0.61, p = 0.03) with serum susceptibility.
  
  
  CONCLUSIONS
  H. pylori are susceptible to complement, alternative pathway activation appears critical, and C3 binding is a major locus of variability.
authors.affiliation: Division of Infectious Diseases, Vanderbilt University School of Medicine, Nashville, TN 37212, USA.
authors.forename: G
authors.initials: G
authors.lastname: Gonzalez-Valencia
authors.forename: G I
authors.initials: GI
authors.lastname: Perez-Perez
authors.forename: R G
authors.initials: RG
authors.lastname: Washburn
authors.forename: M J
authors.initials: MJ
authors.lastname: Blaser
mesh_terms: D000907:Antibodies, Bacterial / Q000276:immunology; D001770:Blood Bactericidal Activity; D002168:Campylobacter fetus / Q000276:immunology; D003167:Complement Activation; D003175:Complement C2 / Q000172:deficiency; D003176:Complement C3 / Q000276:immunology; D011415:Complement Factor B / Q000172:deficiency; D016480:Helicobacter pylori / Q000276:immunology*; D006801:Humans
chemical_list: D000907:Antibodies, Bacterial; D003175:Complement C2; D003176:Complement C3; D011415:Complement Factor B