title: Infection with Chlamydia trachomatis alters the tyrosine phosphorylation and/or localization of several host cell proteins including cortactin.
abstract: Infection of epithelial cells by two biovars of Chlamydia trachomatis results in the tyrosine phosphorylation of several host proteins. The most prominent change in host protein tyrosine phosphorylation involves a complex of proteins with molecular masses of 75 to 85 kDa (pp75/85) and 100 kDa (pp100). The C. trachomatis-induced tyrosine phosphorylation of pp75/85 and pp100 is observed in several cell lines, including epithelial cells, fibroblasts, and macrophages. Subcellular fractionation and detergent solubility properties of pp75/85 are consistent with its association with the cytoskeleton. Phosphoamino acid analysis demonstrates that the pp75/85 complex is phosphorylated on both tyrosine and serine residues. Immunofluorescence studies of chlamydia-infected cells by using fluorescein isothiocyanate-phalloidin and antibodies to phosphotyrosine and cortactin demonstrate that tyrosine-phosphorylated proteins, as well as cortactin, are localized to the chlamydial vacuole and that this process is facilitated by actin.
authors.affiliation: Department of Medicine, University of California, San Francisco 94143-0654, USA.
authors.forename: F S
authors.initials: FS
authors.lastname: Fawaz
authors.forename: C
authors.initials: C
authors.lastname: van Ooij
authors.forename: E
authors.initials: E
authors.lastname: Homola
authors.forename: S C
authors.initials: SC
authors.lastname: Mutka
authors.forename: J N
authors.initials: JN
authors.lastname: Engel
mesh_terms: D000818:Animals; D001692:Biological Transport; D002690:Chlamydia Infections / Q000378:metabolism*; D002692:Chlamydia trachomatis; D051356:Cortactin; D006367:HeLa Cells; D006801:Humans; D051379:Mice; D008840:Microfilament Proteins / Q000378:metabolism*; D010766:Phosphorylation; D011506:Proteins / Q000378:metabolism; D014443:Tyrosine / Q000378:metabolism
chemical_list: C495868:CTTN protein, human; D051356:Cortactin; C495869:Cttn protein, mouse; D008840:Microfilament Proteins; D011506:Proteins; D014443:Tyrosine