Bactericidal activities of povidone-iodine against Mycobacterium.
Three standard strains of Mycobacterium (M. tuberculosis H37Rv, M. avium ATCC15769 and M. kansasii ATCC12478) and 15 clinical isolates of Mycobacterium (7 M. tuberculosis, 2 M. avium, 3 M. kansasii, 1 M. intracellulare, 1 M. chelonae subsp. abscessus and 1 M. gordonae) were selected in order to study the bactericidal activities of povidone-iodine (PVP-I) drug substance and a commercially available PVP-I solution (Isodine solution) against Mycobacterium. After the bacilli had been exposed to the disinfectant solution at concentrations of 0.1 or 0.02% with 2% human serum for various incubation periods from 30 to 120 s, the PVP-I drug substance was inactivated by addition of 0.5% sodium thiosulfate. In the case of the commercially available PVP-I solution, a mixture of 10% Tween 80, 3% soybean lecithin and 0.5% sodium thiosulfate was used as inactivator. It was demonstrated that the 3 standard strains were completely inactivated within 30 s by 0.1% PVP-I drug substance and that the 15 clinical isolates were almost killed by 0.1% commercially available PVP-I solution within 60 s. As a result, the commercially available PVP-I product appeared to be a useful agent as disinfectant against all the tested species of Mycobacterium.
Rikimaru
T
T
Department of Internal Medicine, Kurume University School of Medicine, Japan.
Kondo
M
M
Kondo
S
S
Oizumi
K
K
D000891:Anti-Infective Agents, Local / Q000008:administration & dosage / Q000494:pharmacology*; D001769:Blood; D002614:Chelating Agents / Q000494:pharmacology; D006801:Humans; D007466:Iodophors / Q000008:administration & dosage / Q000494:pharmacology*; D009161:Mycobacterium / Q000187:drug effects*; D009162:Mycobacterium avium / Q000187:drug effects; D015269:Mycobacterium avium Complex / Q000187:drug effects; D016926:Mycobacterium chelonae / Q000187:drug effects; D019909:Mycobacterium kansasii / Q000187:drug effects; D009169:Mycobacterium tuberculosis / Q000187:drug effects; D009170:Nontuberculous Mycobacteria / Q000187:drug effects*; D010713:Phosphatidylcholines / Q000494:pharmacology; D011136:Polysorbates / Q000494:pharmacology; D011206:Povidone-Iodine / Q000008:administration & dosage / Q000494:pharmacology*; D013025:Glycine max; D013501:Surface-Active Agents / Q000494:pharmacology; D013885:Thiosulfates / Q000494:pharmacology; D013997:Time Factors
D000891:Anti-Infective Agents, Local; D002614:Chelating Agents; D007466:Iodophors; D010713:Phosphatidylcholines; D011136:Polysorbates; D013501:Surface-Active Agents; D013885:Thiosulfates; D011206:Povidone-Iodine; C017717:sodium thiosulfate