Kinetic analysis of the Fenton reaction by ESR-spin trapping.
A quantitative analysis of hydroxyl radical (.OH) generated in the Fe(2+)-hydrogen peroxide reaction system was explored by a spin-trapping method using 5,5-dimethyl-1-pyrroline-N-oxide (DMPO) combined with electron spin resonance spectroscopy. Based on the numerical analysis of Fenton-related reactions, the reduction of DMPO-OH adduct from 1:1 stoichiometry prominent at high concentrations of Fe2+ was consistent with a reaction model in which a molar amount of hydrogen peroxide was reduced by two molar amounts of Fe2+. Furthermore, time-dependent decrease in DMPO-OH quantity, apparent at much higher concentration of Fe2+, was proved due to the reaction not with Fe2+ but with Fe3+.
Mizuta
Y
Y
Analytical Instruments Division, Jeol Ltd., Tokyo, Japan.
Masumizu
T
T
Kohno
M
M
Mori
A
A
Packer
L
L
D003497:Cyclic N-Oxides / Q000378:metabolism; D004578:Electron Spin Resonance Spectroscopy; D006861:Hydrogen Peroxide; D017665:Hydroxyl Radical / Q000378:metabolism; D007202:Indicators and Reagents; D007501:Iron; D007700:Kinetics; D010084:Oxidation-Reduction; D013113:Spin Labels; D019097:Spin Trapping / Q000379:methods*
D003497:Cyclic N-Oxides; C045076:Fenton's reagent; D007202:Indicators and Reagents; D013113:Spin Labels; D017665:Hydroxyl Radical; C017245:5,5-dimethyl-1-pyrroline-1-oxide; D006861:Hydrogen Peroxide; D007501:Iron