Repeated exercise induces release of soluble P-selectin in patients with intermittent claudication.
Controversy exists as to whether exercise in patients with intermittent claudication causes a harmful biochemical effect associated with an ischaemia-reperfusion injury of skeletal muscle. We report on exercise-induced changes in neutrophil activation, soluble P-selectin and von Willebrand factor in 34 patients with intermittent claudication and 12 matched controls. Von Willebrand factor (vWF) showed a cyclical pattern of response to exercise in control subjects (rising from 103 +/- 8 to 119 +/- 7 U/dl); claudicants did not show this pattern but had higher levels of vWF throughout (p <0.03). There was no consistent pattern of response in neutrophil hydrogen peroxide production to exercise in either claudicants or control subjects. Soluble P-selectin levels increased after exercise, but this only reached statistical significance after repeated exercise in claudicants (rising from 320 +/- 28 to 357 +/- 28 ng/ml). This rise in soluble P-selectin after exercise may indicate progressive platelet activation which may contribute to the excess cardiovascular mortality that claudicants are prone to.
Kirkpatrick
U J
UJ
Department of Surgery, University Hospital of South Manchester, UK.
Mossa
M
M
Blann
A D
AD
McCollum
C N
CN
D000328:Adult; D000368:Aged; D005080:Exercise Test; D005260:Female; D006801:Humans; D006861:Hydrogen Peroxide / Q000097:blood; D007383:Intermittent Claudication / Q000097:blood* / Q000503:physiopathology*; D007958:Leukocyte Count; D008297:Male; D008875:Middle Aged; D009504:Neutrophils / Q000378:metabolism; D019007:P-Selectin / Q000097:blood*; D005082:Physical Exertion; D012995:Solubility; D014841:von Willebrand Factor / Q000378:metabolism
D019007:P-Selectin; D014841:von Willebrand Factor; D006861:Hydrogen Peroxide