title: May peripheral and central fatigue be correlated? Can we monitor them by means of clinical laboratory tools?
abstract: BACKGROUND
  A laboratory-based model which links regional and central fatigue during physical exercise has not yet developed. Today we can assay the oxypurines, a specific and sensible marker of muscle cell-energy exhaustion during strenuous physical exercise, thus allowing us to insight in peripheral fatigue mechanisms. Prolonged physical exercise modifies plasma free amino acids and fatty acids levels, increases plasma free tryptophan (fTrp) and, conversely, probably serotonin, an amine involved in the genesis of central fatigue. We tried to verify if there is a correlation between central and peripheral fatigue.
  
  
  METHODS
  We studied 29 male marathon runners before marathon, at the arrival, one and three days after the run.
  
  
  METHODS
  Plasma samples were assayed for amino acids, fTrp serotonin, xanthine, hypoxanthine inosine, cortisol. Urine samples were assayed for serotonin and hydroxyin-doleacetic acid (5HIAA).
  
  
  RESULTS
  After the competition we observed a decrease in plasma fTrp but an increased ratio fTrp/sum of neutral amino acids with a normalization after 24 hours. No significant changes were observed in plasma and urinary serotonin and 5HIAA. Hypoxanthine and inosine increased at the end of the trial and returned to basal levels the day after. Cortisol increased at the end of the run but was reduced after 24 and 72 hours.
  
  
  CONCLUSIONS
  In our athletes we observed only indirect signs of fTrp involvement in the genesis of central fatigue. Oxypurines seem to be a good marker of regional muscular fatigue. Plasma cortisol expresses the stress reaction to the competition and its exhaustion after a prolonged physical exercise.
authors.affiliation: Department of Internal Medicine, Cardiology, Hepatology, University of Bologna, Italy.
authors.forename: G P
authors.initials: GP
authors.lastname: Bianchi
authors.forename: G
authors.initials: G
authors.lastname: Grossi
authors.forename: A M
authors.initials: AM
authors.lastname: Bargossi
mesh_terms: D000328:Adult; D000596:Amino Acids / Q000032:analysis* / Q000097:blood / Q000652:urine; D000597:Amino Acids, Branched-Chain / Q000097:blood; D015415:Biomarkers / Q000032:analysis; D002851:Chromatography, High Pressure Liquid; D005221:Fatigue / Q000378:metabolism / Q000503:physiopathology*; D005227:Fatty Acids / Q000097:blood; D005500:Follow-Up Studies; D006801:Humans; D006854:Hydrocortisone / Q000097:blood; D006897:Hydroxyindoleacetic Acid / Q000652:urine; D019271:Hypoxanthine / Q000097:blood; D007124:Immunoenzyme Techniques; D007288:Inosine / Q000097:blood; D008297:Male; D018763:Muscle Fatigue / Q000502:physiology*; D010649:Phenylalanine / Q000097:blood; D005082:Physical Exertion / Q000502:physiology*; D011687:Purines / Q000032:analysis; D012701:Serotonin / Q000097:blood / Q000652:urine; D014364:Tryptophan / Q000097:blood; D014443:Tyrosine / Q000097:blood; D019820:Xanthine / Q000097:blood
chemical_list: D000596:Amino Acids; D000597:Amino Acids, Branched-Chain; D015415:Biomarkers; D005227:Fatty Acids; D011687:Purines; D019820:Xanthine; D019271:Hypoxanthine; D012701:Serotonin; D014443:Tyrosine; D010649:Phenylalanine; D006897:Hydroxyindoleacetic Acid; D007288:Inosine; D014364:Tryptophan; D006854:Hydrocortisone