title: Influence of two blood conservation techniques (cardiotomy reservoir versus cell-saver) on biocompatibility of the heparin coated cardiopulmonary bypass circuit during coronary revascularization surgery.
abstract: Blood conservation during cardiac surgery is critically important because of the inherent risks in homologous blood transfusions. Two techniques for the intraoperative conservation of blood--retransfusion of the red cells using a cell-saver (CS), or retransfusion of the blood using a cardiotomy suction (CTR) system--were compared using biocompatibility markers, granulocyte activation, and production of oxygen-free radicals (OFR). In the CTR group, heparin coated circuits with an uncoated cardiotomy reservoir were used. For the CS group, identical heparin coated cardiopulmonary bypass (CPB) sets, without a cardiotomy reservoir but with a CS, were used. In each group, eight patients had coronary artery bypass grafting performed. The capacity of the whole blood and the granulocytes to produce OFR was estimated by a chemiluminescence, and granulocyte activation was measured as release of the granulocyte granule proteins myeloperoxidase (MPO) and lactoferrin. A significantly reduced capacity to produce OFR by the whole blood was noted at 45 minutes of CPB in the CTR group (68% +/- 17% vs 94% +/- 16% in the CS group). MPO release was higher after 3 hours (p = 0.05) and 20 hours (p < 0.05), postoperatively, in the CTR group (417 +/- 77 micrograms/L and 257 +/- 31 micrograms/L vs 246 +/- 25 micrograms/L and 164 +/- 12 micrograms/L, respectively, in the CS group). We conclude that the heparin coated CPB circuit with the uncoated cardiotomy reservoir may be less biocompatible than the identical CPB set used together with the CS.
authors.affiliation: Department of Cardiothoracic Surgery, University Hospital, Uppsala, Sweden.
authors.forename: J W
authors.initials: JW
authors.lastname: Borowiec
authors.forename: M
authors.initials: M
authors.lastname: Bozdayi
authors.forename: A
authors.initials: A
authors.lastname: Jaramillo
authors.forename: L
authors.initials: L
authors.lastname: Nilsson
authors.forename: P
authors.initials: P
authors.lastname: Venge
authors.forename: A
authors.initials: A
authors.lastname: Henze
mesh_terms: D001672:Biocompatible Materials; D001804:Blood Transfusion, Autologous / Q000295:instrumentation* / Q000379:methods; D002315:Cardiopulmonary Bypass / Q000295:instrumentation*; D001026:Coronary Artery Bypass; D006098:Granulocytes / Q000502:physiology; D006493:Heparin / Q000008:administration & dosage*; D006801:Humans; D007958:Leukocyte Count; D008163:Luminescent Measurements
chemical_list: D001672:Biocompatible Materials; D006493:Heparin