5'-nucleotidase and adenosine deaminase activities in blood of patients with unstable angina pectoris.
During ischaemia and hypoxia adenosine is released from cardiac cells. Adenosine is the end product of 5'-nucleotidase activity. We were interested in how this enzyme activity in plasma of patients with unstable angina pectoris, causes short-term ischaemia. 5'-Nucleotidase activity in plasma was determined using a standard diagnostic kit from Sigma. Furthermore, we studied the activity of adenosine deaminase in plasma, granulocytes, lymphocytes and erythrocytes by the methods of Hopkinson [1]. It was found that 5'-nucleotidase activity was increased by about 43% in plasma. The activity of adenosine deaminase (ADA) in plasma increased by 6%, but in granulocytes, lymphocytes and erythrocytes decreased by about 24, 19 and 10.6%, respectively. We concluded that a large increase in 5'-nucleotidase activity may be caused by activation of 5'-ectonucleotidase in blood cells by ischaemia. However, the decrease in ADA activity in blood cells may be associate with the adenosine metabolism.
Kopff
M
M
Department of Biochemistry, Military Medical Academy, Lódź, Poland.
Zakrzewska
I
I
Fuchs-Kalinowska
J
J
Klem
J
J
Strzelczyk
M
M
Puczkowski
S
S
D015720:5'-Nucleotidase / Q000097:blood*; D000243:Adenosine Deaminase / Q000097:blood*; D000249:Adenosine Monophosphate / Q000097:blood; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000789:Angina, Unstable / Q000097:blood* / Q000201:enzymology*; D004789:Enzyme Activation; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D010766:Phosphorylation
D000249:Adenosine Monophosphate; D015720:5'-Nucleotidase; D000243:Adenosine Deaminase