Determination of phloxine B and uranine in water by capillary zone electrophoresis.
Phloxine B and uranine are color additives in drugs and cosmetics as well as potential photoactive insecticides. A capillary zone electrophoretic (CZE) method is developed to determine phloxine B and uranine in water. A fused-silica capillary (67 cm, 75-micron i.d.) and borate buffer are used. Migration of phloxine B and uranine increases slightly as the pH of the running buffer increases between the range of 8-9. Although there are only slight effects of ionic strength on the analyte migration in the range of 0-20 mM NaCl in the running buffer, the migration of phloxine B and uranine increases as the percentage of methanol in the samples increases. Methanol shows little effect on the quantitation of phloxine B and uranine. The CZE procedure is applied to determine phloxine B and uranine fortified in tap and stream water samples. Solid-phase extraction is employed to recover the analytes spiked in the water samples. Recoveries range from 87-112% for phloxine B spiked at 10-200 ppb in the tap and stream water. Uranine recoveries are 86-91% at fortification levels of 10-50 ppb in the water samples.
Li
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QX
Department of Environmental Biochemistry, University of Hawaii, Honolulu 96822, USA.
Alcantara-Licudine
J P
JP
Li
L P
LP
D002021:Buffers; D003287:Contrast Media / Q000032:analysis* / Q000737:chemistry; D019075:Electrophoresis, Capillary / Q000379:methods*; D010697:Eosine I Bluish / Q000032:analysis* / Q000737:chemistry; D019793:Fluorescein / Q000032:analysis* / Q000737:chemistry; D005456:Fluorescent Dyes / Q000032:analysis* / Q000737:chemistry; D006863:Hydrogen-Ion Concentration; D000432:Methanol / Q000737:chemistry; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013050:Spectrometry, Fluorescence; D014867:Water / Q000737:chemistry*
D002021:Buffers; D003287:Contrast Media; D005456:Fluorescent Dyes; D014867:Water; D010697:Eosine I Bluish; D019793:Fluorescein; D000432:Methanol