Expression cloning and characterization of a renal organic anion transporter from winter flounder.
The cDNA coding for a renal p-aminohippurate (PAH) transporter from winter flounder (Pseudopleuronectes americanus), designated fROAT, was cloned by functional expression in Xenopus laevis oocytes. fROAT is approximately 2.8 kbp in length and encodes a protein of 562 amino acids, related to the rat renal organic anion transporter ROAT1/OAT1 and the organic cation transporters OCT1 and OCT2. In oocytes, fROAT mediated probenecid-sensitive PAH uptake, with a Km for PAH of about 20 microM, and inhibited by external glutarate (GA) (1 mM). The functional characteristics suggest that fROAT is the basolateral PAH/dicarboxylate exchanger of the flounder kidney.
Wolff
N A
NA
Abteilung Vegetative Physiologie, Zentrum Physiologie und Pathophysiologie, Georg-August-Universität Göttingen, Germany. nwolff@veg-physiol.med.uni-goettingen.de
Werner
A
A
Burkhardt
S
S
Burckhardt
G
G
D000595:Amino Acid Sequence; D000818:Animals; D027321:Anion Transport Proteins; D001483:Base Sequence; D002352:Carrier Proteins / Q000096:biosynthesis / Q000737:chemistry* / Q000494:pharmacology*; D003001:Cloning, Molecular; D005260:Female; D005432:Flounder; D015723:Gene Library; D007668:Kidney / Q000378:metabolism*; D007700:Kinetics; D008969:Molecular Sequence Data; D009865:Oocytes / Q000502:physiology; D012333:RNA, Messenger / Q000302:isolation & purification / Q000378:metabolism; D051381:Rats; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid; D014982:Xenopus laevis; D010130:p-Aminohippuric Acid / Q000493:pharmacokinetics*
D027321:Anion Transport Proteins; D002352:Carrier Proteins; D012333:RNA, Messenger; D010130:p-Aminohippuric Acid