title: Mosquito larvicidal activity of transgenic Anabaena strain PCC 7120 expressing combinations of genes from Bacillus thuringiensis subsp. israelensis.
abstract: Various combinations of the genes cryIVA (cry4A), cryIVD (cry11A), and p20 from Bacillus thuringiensis subsp. israelensis were introduced into the nitrogen-fixing cyanobacterium Anabaena sp. strain PCC 7120 by means of Escherichia coli-Anabaena shuttle vector pRL488p and were expressed under control of two tandem strong promoters, a cyanobacterial promoter (PpsbA) and an E. coli T7 promoter (PA1). Two of the clones carrying cryIVA plus cryIVD, one with p20 and one without p20, displayed toxicity against third-instar larvae of Aedes aegypti at levels greater than any level previously reported for transgenic cyanobacteria.
authors.affiliation: Blaustein Institute for Desert Research, Ben-Gurion University at Sede-Boker, Israel.
authors.forename: W
authors.initials: W
authors.lastname: Xiaoqiang
authors.forename: S J
authors.initials: SJ
authors.lastname: Vennison
authors.forename: L
authors.initials: L
authors.lastname: Huirong
authors.forename: E
authors.initials: E
authors.lastname: Ben-Dov
authors.forename: A
authors.initials: A
authors.lastname: Zaritsky
authors.forename: S
authors.initials: S
authors.lastname: Boussiba
mesh_terms: D000330:Aedes; D017033:Anabaena / Q000235:genetics*; D000818:Animals; D001413:Bacillus thuringiensis / Q000235:genetics*; D000083722:Bacillus thuringiensis Toxins; D001426:Bacterial Proteins / Q000235:genetics* / Q000494:pharmacology; D001427:Bacterial Toxins; D004731:Endotoxins / Q000235:genetics* / Q000494:pharmacology; D004926:Escherichia coli / Q000235:genetics; D015870:Gene Expression; D018014:Gene Transfer Techniques; D005798:Genes, Bacterial; D005822:Genetic Vectors; D006460:Hemolysin Proteins; D007814:Larva; D010572:Pest Control, Biological; D011401:Promoter Regions, Genetic
chemical_list: D000083722:Bacillus thuringiensis Toxins; D001426:Bacterial Proteins; D001427:Bacterial Toxins; D004731:Endotoxins; D006460:Hemolysin Proteins; C030956:insecticidal crystal protein, Bacillus Thuringiensis