A comparison of hepatic cytochrome P450 protein expression between infancy and postinfancy.
We immunochemically measured the contents of 9 different cytochrome P450 (CYP) isoenzymes expressed in the liver and compared them between two groups: one group of 6 infant and 4 perinatal patients and one group of 10 patients after infancy (over 1 year old). CYP protein expressed in human liver can be divided into three groups on the basis of expression pattern: (a) CYP2A6, 2C9, 2D6, 2E1, and 3A were present in all samples and no difference was observed between the two groups; (b) CYP1A2, 2B6, and 2C8 were expressed more after infancy than during infancy; and (c) CYP3A7, which has been considered a major CYP enzyme in fetal liver microsomes, was expressed in all infants as well as the four perinatal patients, whereas it was detected in only 2 patients after infancy. These results implied that CYP2A6, 2C9, 2D6, 2E1, and 3A are already expressed during perinatal and infant period, while CYP1A2, 2B6, and 2C8 are expressed highly in subjects over 1 year old, and CYP3A7 disappeared after infancy.
Tateishi
T
T
Department of Pharmacology, St. Marianna University School of Medicine, Kanagawa, Japan.
Nakura
H
H
Asoh
M
M
Watanabe
M
M
Tanaka
M
M
Kumai
T
T
Takashima
S
S
Imaoka
S
S
Funae
Y
Y
Yabusaki
Y
Y
Kamataki
T
T
Kobayashi
S
S
D000293:Adolescent; D000328:Adult; D000368:Aged; D000375:Aging / Q000378:metabolism*; D015153:Blotting, Western; D002648:Child; D002675:Child, Preschool; D003577:Cytochrome P-450 Enzyme System / Q000378:metabolism*; D005260:Female; D005333:Fetus / Q000378:metabolism; D005865:Gestational Age; D006801:Humans; D007223:Infant; D007231:Infant, Newborn; D007527:Isoenzymes / Q000378:metabolism*; D008099:Liver / Q000196:embryology / Q000201:enzymology* / Q000254:growth & development; D008297:Male; D008875:Middle Aged
D007527:Isoenzymes; D003577:Cytochrome P-450 Enzyme System