title: Amphistin, a new melanogenesis inhibitor, produced by an actinomycete.
abstract: A new melanogenesis inhibitor, named amphistin, was isolated from the fermentation broth of an actinomycete strain KP-3052. Amphistin was purified from the culture filtrate by the combination of cation exchange, gel filtration, and aminosilyl silica gel chromatographic methods. The structure of amphistin was elucidated as gamma-(beta-histidinoalanino)homoalanine by NMR experiments including 1H-15N HMBC experiment and other spectroscopic analyses. Amphistin inhibited the melanogenesis of B16 melanoma cells at concentration of 6.8 microM.
authors.affiliation: Research Center for Biological Function, Kitasato Institute, Tokyo, Japan.
authors.forename: N
authors.initials: N
authors.lastname: Arai
authors.forename: K
authors.initials: K
authors.lastname: Shiomi
authors.forename: S
authors.initials: S
authors.lastname: Takamatsu
authors.forename: K
authors.initials: K
authors.lastname: Komiyama
authors.forename: M
authors.initials: M
authors.lastname: Shinose
authors.forename: Y
authors.initials: Y
authors.lastname: Takahashi
authors.forename: Y
authors.initials: Y
authors.lastname: Tanaka
authors.forename: Y
authors.initials: Y
authors.lastname: Iwai
authors.forename: J R
authors.initials: JR
authors.lastname: Liu
authors.forename: S
authors.initials: S
authors.lastname: Omura
mesh_terms: D000191:Actinomycetaceae / Q000737:chemistry / Q000378:metabolism*; D000613:Aminobutyrates / Q000737:chemistry* / Q000378:metabolism / Q000494:pharmacology*; D000818:Animals; D001419:Bacteria / Q000187:drug effects; D005285:Fermentation; D005658:Fungi / Q000187:drug effects; D007093:Imidazoles / Q000737:chemistry* / Q000378:metabolism / Q000494:pharmacology*; D008543:Melanins / Q000096:biosynthesis*; D008546:Melanoma, Experimental; D051379:Mice; D008826:Microbial Sensitivity Tests; D015394:Molecular Structure; D014407:Tumor Cells, Cultured
chemical_list: D000613:Aminobutyrates; D007093:Imidazoles; D008543:Melanins; C109696:amphistin