title: The irradiation of hepatocytes with He-Ne laser causes an increase of cytosolic free calcium concentration and an increase of cell membrane potential, correlated with it, both increases taking place in an oscillatory manner.
abstract: Isolated hepatocytes were irradiated with Helium-Neon laser (fluence: 0.24 Joules x cm-2, fluence rate: 12 mW x cm-2) and changes of both cytosolic free Ca2+ concentration and cell membrane potential were checked by measuring fura-2 and bis-oxonol fluorescence respectively. Irradiation resulted in an enhancement in cytosolic free Ca2+ concentration that requires the presence of Ca2+ in the phase outside hepatocytes; consistently an increase in cell membrane potential was measured correlated with it. Interestingly, the rate of increase of both cytosolic free Ca2+ concentration and cell membrane potential shows special time dependent features similar to those peculiar of oscillatory processes.
authors.affiliation: Centro Studi Mitocondri e Metabolismo Energetico, C.N.R., Bari, Italy.
authors.forename: R A
authors.initials: RA
authors.lastname: Vacca
authors.forename: L
authors.initials: L
authors.lastname: Moro
authors.forename: V A
authors.initials: VA
authors.lastname: Petragallo
authors.forename: M
authors.initials: M
authors.lastname: Greco
authors.forename: F
authors.initials: F
authors.lastname: Fontana
authors.forename: S
authors.initials: S
authors.lastname: Passarella
mesh_terms: D000818:Animals; D001683:Biological Clocks / Q000528:radiation effects*; D002118:Calcium / Q000378:metabolism* / Q000502:physiology / Q000528:radiation effects*; D002462:Cell Membrane / Q000528:radiation effects; D003600:Cytosol / Q000378:metabolism* / Q000502:physiology / Q000528:radiation effects*; D006371:Helium; D007834:Lasers; D008027:Light; D008099:Liver / Q000166:cytology / Q000502:physiology* / Q000528:radiation effects*; D008297:Male; D008564:Membrane Potentials / Q000528:radiation effects; D009356:Neon; D051381:Rats; D017208:Rats, Wistar
chemical_list: D006371:Helium; D009356:Neon; D002118:Calcium