title: Phosphorylation of Enabled by the Drosophila Abelson tyrosine kinase regulates the in vivo function and protein-protein interactions of Enabled.
abstract: Drosophila Enabled (Ena) is a member of a family of cytoskeleton-associated proteins including mammalian vasodilator-stimulated phosphoprotein and murine Enabled that regulate actin cytoskeleton assembly. Mutations in Drosophila ena were discovered as dominant genetic suppressors of mutations in the Abelson tyrosine kinase (Abl), suggesting that Ena and Abl function in the same pathway or process. We have identified six tyrosine residues on Ena that are phosphorylated by Abl in vitro and in vivo. Mutation of these phosphorylation sites to phenylalanine partially impaired the ability of Ena to restore viability to ena mutant animals, indicating that phosphorylation is required for optimal Ena function. Phosphorylation of Ena by Abl inhibited the binding of Ena to SH3 domains in vitro, suggesting that one effect of Ena phosphorylation may be to modulate its association with other proteins.
authors.affiliation: McArdle Laboratory for Cancer Research and Laboratory of Genetics, University of Wisconsin-Madison, 53706, USA.
authors.forename: A R
authors.initials: AR
authors.lastname: Comer
authors.forename: S M
authors.initials: SM
authors.lastname: Ahern-Djamali
authors.forename: J L
authors.initials: JL
authors.lastname: Juang
authors.forename: P D
authors.initials: PD
authors.lastname: Jackson
authors.forename: F M
authors.initials: FM
authors.lastname: Hoffmann
mesh_terms: D000818:Animals; D004268:DNA-Binding Proteins / Q000235:genetics* / Q000378:metabolism; D004330:Drosophila / Q000235:genetics* / Q000378:metabolism; D016313:Genes, abl; D009154:Mutation; D010766:Phosphorylation; D011485:Protein Binding; D015398:Signal Transduction / Q000235:genetics; D018909:src Homology Domains / Q000235:genetics
chemical_list: D004268:DNA-Binding Proteins; C092744:ENA-VASP proteins