title: Microchromatographic quantitation of hemoglobin A levels in phenotypes of sickle cell-beta(+) thalassemia.
abstract: The inheritance of the sickle cell gene in combination with a gene for beta(+) thalassemia results in a spectrum of sickle cell-beta(+) thalassemia syndromes with varying levels of hemoglobin A (HbA). Some severe sickle cell-beta(+) thalassemia syndromes have small amounts of HbA, which may be difficult to quantitate in the presence of fetal hemoglobin. A microcolumn chromatographic method, using 0.5 M Tris-acetic acid developers with varying pH values from 9.0 to 6.0, appears to adequately quantitate small amounts of HbA. This method is relatively simple and cheaper than high-performance liquid chromatography, a major consideration in developing countries.
authors.affiliation: Department of Pathology, University of the West Indies, Kingston, Jamaica.
authors.forename: I
authors.initials: I
authors.lastname: Sweeting
authors.forename: B E
authors.initials: BE
authors.lastname: Serjeant
authors.forename: P W
authors.initials: PW
authors.lastname: Thomas
authors.forename: G R
authors.initials: GR
authors.lastname: Serjeant
mesh_terms: D002845:Chromatography / Q000295:instrumentation; D002851:Chromatography, High Pressure Liquid; D005319:Fetal Hemoglobin / Q000032:analysis; D006441:Hemoglobin A / Q000032:analysis*; D006801:Humans; D007231:Infant, Newborn; D010641:Phenotype; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013577:Syndrome; D017086:beta-Thalassemia / Q000097:blood* / Q000235:genetics
chemical_list: D006441:Hemoglobin A; D005319:Fetal Hemoglobin