Capillary electrophoresis procedures for serum protein analysis: comparison with established techniques.
Methods using automated capillary electrophoresis (CE) instrumentation are available for serum protein electrophoresis with monoclonal band quantitation, isoelectric focusing and sodium dodecyl sulphate-polyacrylamide gel electrophoresis separations. The advantages of CE over previous gel methods relate to the time and labour saved by the automated instrumentation. High pI monoclonal bands and cryoglobulin specimens can be successfully analysed by CE. However, if the CE application uses a standard company supplied kit, then the cost savings are often negated by the high cost of the kit. Improvements such as the inclusion of both a UV-Vis as well as a fluorescence detector as standard within the one commercial instrument, the production of coated IEF capillaries with a useful life of at least 100 samples, and the introduction of a capillary array into all commercial instrumentation would ensure greater use of CE within both the clinical and other protein laboratories.
Jenkins
M A
MA
Division of Laboratory Medicine, Austin and Repatriation Medical Center, Heidelberg, Vic., Australia.
Guerin
M D
MD
D000911:Antibodies, Monoclonal / Q000276:immunology; D001797:Blood Protein Electrophoresis / Q000379:methods*; D001798:Blood Proteins / Q000032:analysis* / Q000276:immunology; D003450:Cryoglobulins / Q000032:analysis / Q000276:immunology; D004587:Electrophoresis, Agar Gel; D019075:Electrophoresis, Capillary / Q000379:methods*; D004591:Electrophoresis, Polyacrylamide Gel; D007122:Immunoelectrophoresis; D007525:Isoelectric Focusing; D012680:Sensitivity and Specificity
D000911:Antibodies, Monoclonal; D001798:Blood Proteins; D003450:Cryoglobulins