Simultaneous determination of S-adenosylmethionine and S-adenosylhomocysteine by capillary zone electrophoresis.
A reproducible, rapid procedure for the simultaneous quantitative separation of S-adenosylmethionine and S-adenosylhomocysteine by capillary zone electrophoresis has been developed. Separations were performed by using an uncoated capillary of 60 cm effective length and 50 microm ID, 40 mM sodium phosphate buffer, pH 2.50, as background electrolyte solution, and 30 kV. On-line detection was carried out at 254 nm. Under the conditions selected we resolved a standard solution containing S-adenosylmethionine and S-adenosylhomocysteine in a run time shorter than 8 min. A mass detection limit in the range of 10 fmol was achieved. Adenosyl-L-methionine, S[methyl-3H] has also been assayed under the same experimental conditions. Other related compounds did not show interference, including those derived from the hydrolysis of S-adenosylmethionine. The present method allows simultaneous determination of these compounds, which play an important role in many microbiological and enzymatic research studies.
Pañak
K C
KC
Cátedra de Química Analítica Instrumental, Facultad de Farmacia y Bioquímica, Universidad de Buenos Aires, Argentina.
Giorgieri
S A
SA
Díaz
L E
LE
Ruiz
O A
OA
D002021:Buffers; D019075:Electrophoresis, Capillary / Q000379:methods*; D010710:Phosphates; D011786:Quality Control; D015203:Reproducibility of Results; D012435:S-Adenosylhomocysteine / Q000032:analysis*; D012436:S-Adenosylmethionine / Q000032:analysis*; D012680:Sensitivity and Specificity
D002021:Buffers; D010710:Phosphates; D012436:S-Adenosylmethionine; D012435:S-Adenosylhomocysteine; C018279:sodium phosphate