HPLC/circular dichroism detection using a split-type flow cell for polarized photometric detector.
HPLC/circular dichroism detection using a conventional spectrophotometric detector has been established. A split-type flow cell developed for a polarized photometric detector, which is a non-modulated polarimeter, was inserted into this detector. When two rays of circularly polarized light with opposite rotational senses to each other were incident in both cells by combining a polarizer and a 1/4 wave retarder in front of the cell assembly, the output from this spectrophotometric detector became solely the signal based on circular dichroism. Selective detection of a chiral analyte, in which the ratio of the difference in the absorbance between two rays mentioned above and the absorbance by normal rays in over 1/1000, is feasible using the proposed instrument.
Yamamoto
A
A
Toyama Institute of Health, Japan.
Matsunaga
A
A
Hayakawa
K
K
Nishimura
M
M
D002851:Chromatography, High Pressure Liquid / Q000295:instrumentation / Q000379:methods*; D002942:Circular Dichroism; D012680:Sensitivity and Specificity; D013056:Spectrophotometry, Ultraviolet / Q000295:instrumentation*