title: Persistent infection by Helicobacter pylori down-modulates virus-specific CD8+ cytotoxic T cell response and prolongs viral infection.
abstract: To determine whether Helicobacter pylori infection affects clearance of a concomitant viral infection and cytotoxic T lymphocyte (CTL) and cytokine response to that infection, H. pylori-infected BALB/c mice were challenged with a recombinant vaccinia virus expressing human immunodeficiency virus type 1 gp160. Two H. pylori strains, a colonizing clinical isolate (KS612) and an established standard noncolonizing strain (NCTC11637), were compared. Clearance of recombinant vaccinia virus was reduced in KS612-infected mice compared with NCTC11637-infected and control mice. As a potential mechanism, in contrast to control or NCTC11637-infected mice, the H. pylori clinical isolate KS612 diminished gp160-specific and vaccinia virus-specific CTL activity, even in the presence of exogenous interleukin-2. Furthermore, KS612-infected mice had reduced Th1 cytokine responses to gp120 in vitro compared with control or NCTC11637-infected mice. These results have implications for possible effects of prevalent H. pylori infection on other human diseases.
authors.affiliation: Department of Microbiology, Yamaguchi University School of Medicine, Japan.
authors.forename: M
authors.initials: M
authors.lastname: Shirai
authors.forename: T
authors.initials: T
authors.lastname: Arichi
authors.forename: T
authors.initials: T
authors.lastname: Nakazawa
authors.forename: J A
authors.initials: JA
authors.lastname: Berzofsky
mesh_terms: D000818:Animals; D000942:Antigens, Bacterial / Q000276:immunology; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology / Q000378:metabolism; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology / Q000378:metabolism; D003601:Cytotoxicity Tests, Immunologic; D005260:Female; D006678:HIV / Q000502:physiology; D019302:HIV Envelope Protein gp160 / Q000235:genetics / Q000276:immunology; D015658:HIV Infections / Q000150:complications* / Q000235:genetics / Q000276:immunology*; D016481:Helicobacter Infections / Q000150:complications* / Q000276:immunology*; D016480:Helicobacter pylori / Q000276:immunology / Q000472:pathogenicity; D007371:Interferon-gamma / Q000276:immunology / Q000378:metabolism; D007376:Interleukin-2 / Q000276:immunology / Q000378:metabolism / Q000494:pharmacology; D015847:Interleukin-4 / Q000276:immunology / Q000378:metabolism; D008099:Liver / Q000821:virology; D051379:Mice; D008807:Mice, Inbred BALB C; D011995:Recombination, Genetic; D013047:Specific Pathogen-Free Organisms; D013154:Spleen / Q000276:immunology / Q000821:virology; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology*; D014616:Vaccinia virus / Q000235:genetics; D017735:Virus Latency
chemical_list: D000942:Antigens, Bacterial; D019302:HIV Envelope Protein gp160; D007376:Interleukin-2; D015847:Interleukin-4; D007371:Interferon-gamma