Hepatoprotective principles of Swertia japonica Makino on D-galactosamine/lipopolysaccharide-induced liver injury in mice.
The n-BuOH extract of Swertia japonica showed a significant hepatoprotective effect on D-galactosamine (D-GalN)/lipopolysaccharide (LPS)-induced liver injury in mice. The activity-guided fractionation led to the isolation of a new tetrahydroxanthone derivative, tetrahydroswertianolin (1), as well as two known iridoids, gentiopicroside (2) and sweroside (3). Their structures were elucidated by spectroscopic methods and chemical reactions. Of the three compounds, 2 and 3 possessed mild hepatoprotective activity at a dose range of 25-50 mg/kg, whereas, 1 exhibited potent activity in a dose-dependent manner. The hepatoprotective effect of tetrahydroswertianolin (1) was stronger than that of glycyrrhizin which was used as a positive control.
Hase
K
K
Research Institute for Wakan-Yaku (Traditional Sino-Japanese Medicines), Toyama Medical and Pharmaceutical University, Japan.
Li
J
J
Basnet
P
P
Xiong
Q
Q
Takamura
S
S
Namba
T
T
Kadota
S
S
D000818:Animals; D056486:Chemical and Drug Induced Liver Injury / Q000517:prevention & control*; D005688:Galactosamine / Q000037:antagonists & inhibitors / Q000633:toxicity; D005960:Glucosides / Q000302:isolation & purification / Q000494:pharmacology; D057888:Iridoid Glucosides; D039823:Iridoids; D008070:Lipopolysaccharides / Q000037:antagonists & inhibitors / Q000633:toxicity; D009682:Magnetic Resonance Spectroscopy; D008297:Male; D051379:Mice; D008815:Mice, Inbred Strains; D010936:Plant Extracts / Q000494:pharmacology*; D010946:Plants, Medicinal / Q000737:chemistry; D016339:Spectrometry, Mass, Fast Atom Bombardment; D013055:Spectrophotometry, Infrared; D013056:Spectrophotometry, Ultraviolet
D005960:Glucosides; D057888:Iridoid Glucosides; D039823:Iridoids; D008070:Lipopolysaccharides; D010936:Plant Extracts; C109887:tetrahydroswertianolin; D005688:Galactosamine