Use of EBV-based Vector/HVJ-liposome complex vector for targeted gene therapy of EBV-associated neoplasms.
Targeted suicide gene therapy for Epstein-Barr virus (EBV)-associated neoplasms was attempted by using EBV-based plasmid vectors coupled with hemagglutinating virus of Japan (HVJ)-liposome in vitro. Expression of EBV nuclear antigen (EBNA)1 is a common feature of the neoplasms associated with EBV. When various leukemic cell lines were transduced with a vector carrying a marker gene and EBV replication origin of plasmid (oriP), the marker gene product was exclusively detected in cells expressing EBNA1. Transduction of herpes simplex virus (HSV)-1 thymidine kinase (Tk) gene resulted in a marked reduction in viable cell number by ganciclovir (GCV) specifically in EBNA1 positive cells. The results demonstrate that this virus-free system may be applicable to gene therapy of EBV-associated neoplasms.
Hirai
H
H
Second Department of Medicine, Kyoto Prefectural University of Medicine, Kyoto, 602, Japan.
Satoh
E
E
Osawa
M
M
Inaba
T
T
Shimazaki
C
C
Kinoshita
S
S
Nakagawa
M
M
Mazda
O
O
Imanishi
J
J
D000998:Antiviral Agents / Q000633:toxicity; D002051:Burkitt Lymphoma; D017931:DNA Primers; D018274:Electroporation; D019309:Epstein-Barr Virus Nuclear Antigens / Q000096:biosynthesis; D015774:Ganciclovir / Q000633:toxicity; D015316:Genetic Therapy / Q000379:methods*; D005822:Genetic Vectors; D018259:Herpesvirus 1, Human / Q000235:genetics; D004854:Herpesvirus 4, Human / Q000235:genetics / Q000502:physiology; D006801:Humans; D004915:Leukemia, Erythroblastic, Acute; D008081:Liposomes; D016133:Polymerase Chain Reaction; D018741:Replication Origin; D018108:Respirovirus; D013937:Thymidine Kinase / Q000096:biosynthesis; D014162:Transfection / Q000379:methods*
D000998:Antiviral Agents; D017931:DNA Primers; D019309:Epstein-Barr Virus Nuclear Antigens; D008081:Liposomes; D013937:Thymidine Kinase; D015774:Ganciclovir