[Amylase production by Aureobasidium pullulans in liquid and solid media].
Amylase production by a strain of Aureobasidium pullulans isolated in the laboratory was evaluated in liquid media (complex and synthetic) and in solid medium (wheat bran). There was an inhibitory effect in amylase production or amylase secretion by glucose. Asparagine was the best nitrogen source for amylase production (4-6 g/l). Only chlamidospores and melanin but not, amylase activity, were obtained with ammonium sulfate. Amylase production in solid culture was higher than the production obtained in the liquid media assayed. Optimum initial moisture content in solid culture ranged between 57 and 74%. No difference was observed in amylase production between solid media inoculated with cells grown in liquid or solid media.
Lodato
P B
PB
Departamento de Ingeniería Química, Facultad de Ingeniería, Universidad de Buenos Aires, Argentina.
Forchiassin
F
F
Segovia de Huergo
M B
MB
D003470:Culture Media / Q000494:pharmacology; D004790:Enzyme Induction; D005656:Fungal Proteins / Q000096:biosynthesis*; D005087:Glucan 1,4-alpha-Glucosidase / Q000096:biosynthesis*; D003904:Mitosporic Fungi / Q000187:drug effects / Q000201:enzymology* / Q000254:growth & development; D000516:alpha-Amylases / Q000096:biosynthesis*
D003470:Culture Media; D005656:Fungal Proteins; D000516:alpha-Amylases; D005087:Glucan 1,4-alpha-Glucosidase