title: An established hybridoma clone producing a monoclonal antibody against Vibrio anguillarum.
abstract: Vibrio anguillarum is a pathogenic microorganism of vibriosis, an infectious disease found in various fish species. A mouse hybridoma clone, named C5, that produced a monoclonal antibody to V. anguillarum was established. The specific reaction of C5 antibody with V. anguillarum was confirmed by the pre-adsorption effect of the V. anguillarum cells in ELISA and a cell immunoprecipitation experiment. Western blotting analysis indicated that the C5 antibody recognized a high molecular weight substance extracted from cells with detergents.
authors.affiliation: Kyoto Institute of Technology, Japan.
authors.forename: K
authors.initials: K
authors.lastname: Ikura
authors.forename: S
authors.initials: S
authors.lastname: Kodama
authors.forename: H
authors.initials: H
authors.lastname: Hashimoto
authors.forename: K
authors.initials: K
authors.lastname: Hayashi
authors.forename: H
authors.initials: H
authors.lastname: Mori
authors.forename: M
authors.initials: M
authors.lastname: Ichida
authors.forename: M
authors.initials: M
authors.lastname: Sorimachi
authors.forename: K
authors.initials: K
authors.lastname: Kudo
authors.forename: S
authors.initials: S
authors.lastname: Hara
mesh_terms: D000818:Animals; D000907:Antibodies, Bacterial / Q000096:biosynthesis* / Q000276:immunology / Q000494:pharmacology; D000911:Antibodies, Monoclonal / Q000096:biosynthesis* / Q000276:immunology / Q000494:pharmacology; D000942:Antigens, Bacterial / Q000276:immunology; D004591:Electrophoresis, Polyacrylamide Gel; D004797:Enzyme-Linked Immunosorbent Assay; D006825:Hybridomas / Q000378:metabolism*; D051379:Mice; D008807:Mice, Inbred BALB C; D012967:Sodium Dodecyl Sulfate; D014733:Vibrio / Q000276:immunology*
chemical_list: D000907:Antibodies, Bacterial; D000911:Antibodies, Monoclonal; D000942:Antigens, Bacterial; D012967:Sodium Dodecyl Sulfate