title: Oxidative cross-linking of plasma membrane arabinogalactan proteins.
abstract: Monoclonal antibodies which recognize carbohydrate in arabinogalactan proteins (AGPs) have revealed that certain carbohydrate epitopes at the outer plasma membrane surface are developmentally regulated. Some epitopes are expressed according to cell position, and AGPs are thought to play a role in cell-cell interaction during development. This study demonstrates that sugar beet plasma membranes contain two subfamilies of AGPs, with apparent molecular masses of 82 and 97 kDa, and that each subfamily consists of a small number of acidic AGP isoforms. Excision of leaves generates three additional AGP complexes with apparent molecular masses of 120, 170 and 210 kDa, with the 170 kDa complex being the major form induced by excision. The addition of millimolar concentrations of H2O2 to a partially purified fraction of the 82 and 97 kDa AGPs also generates AGP complexes, with the 170 kDa complex as the major form. These results indicate that the plasma membrane AGPs are a target for endogenous H2O2.
authors.affiliation: Department of Plant Biochemistry, Lund University, Sweden. per.kjellbom@plantbio.lu.se
authors.forename: P
authors.initials: P
authors.lastname: Kjellbom
authors.forename: L
authors.initials: L
authors.lastname: Snogerup
authors.forename: C
authors.initials: C
authors.lastname: Stöhr
authors.forename: C
authors.initials: C
authors.lastname: Reuzeau
authors.forename: P F
authors.initials: PF
authors.lastname: McCabe
authors.forename: R I
authors.initials: RI
authors.lastname: Pennell
mesh_terms: D002462:Cell Membrane / Q000378:metabolism; D003432:Cross-Linking Reagents; D004591:Electrophoresis, Polyacrylamide Gel; D008970:Molecular Weight; D009088:Mucoproteins / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism*; D018515:Plant Leaves / Q000502:physiology; D010940:Plant Proteins / Q000378:metabolism; D010945:Plants, Edible / Q000502:physiology*
chemical_list: D003432:Cross-Linking Reagents; D009088:Mucoproteins; D010940:Plant Proteins; C006619:arabinogalactan proteins