Acid steatocrit: a simple, rapid gravimetric method to determine steatorrhea.
OBJECTIVE
The detection and evaluation of steatorrhea in a rapid, quantitative fashion are clinically needed in patients with suspected steatorrhea. Our aim was to evaluate the acid steatocrit method, on random spot stools in adults with and without steatorrhea, relative to the qualitative (microscopic) and quantitative assessments for fecal fat.


METHODS
Stool samples were collected 72 h after a diet of 100 g of fat per day and randomly from 15 healthy controls, 14 patients with chronic pancreatitis, and seven patients with small bowel disease. All stools had quantitative, qualitative, and acid steatocrit analyses performed for fecal fat.


RESULTS
The sensitivity and specificity for the detection of steatorrhea by the spot stool qualitative fecal fat were 78 and 70%, respectively. The spot stool acid steatocrit correlated linearly with the 72-h stool quantitative fecal fat (g/24 h), r = 0.761 and p < 0.001. The acid steatocrit on random spot stools, compared with the 72-h stool quantitative fecal fat, revealed a sensitivity of 100%, a specificity of 95%, and a positive predictive value of 90% for the detection of steatorrhea. It also estimated the quantitative fecal fat.


CONCLUSIONS
The acid steatocrit can be performed accurately on random spot stools and can be used to detect the presence of steatorrhea and estimate the quantitative fecal fat. This assay can be done with readily available equipment for rapid evaluation. Use of a spot stool sample simplifies the acid steatocrit, further improving on the practicality of this test. This study also confirms the clinical usefulness of this simplified method to detect steatorrhea.
Amann
S T
ST
Department of Medicine, University of Florida, Gainesville 32610-0214, USA.
Josephson
S A
SA
Toskes
P P
PP
D000328:Adult; D000368:Aged; D002446:Celiac Disease / Q000175:diagnosis*; D002498:Centrifugation; D002908:Chronic Disease; D004041:Dietary Fats / Q000008:administration & dosage / Q000378:metabolism; D005243:Feces / Q000737:chemistry*; D005260:Female; D006801:Humans; D007410:Intestinal Diseases / Q000378:metabolism; D007421:Intestine, Small; D008055:Lipids / Q000032:analysis*; D008297:Male; D008853:Microscopy; D008875:Middle Aged; D010195:Pancreatitis / Q000378:metabolism; D011237:Predictive Value of Tests; D012680:Sensitivity and Specificity; D013997:Time Factors
D004041:Dietary Fats; D008055:Lipids