Dual image of myosin and actin in human neutrophils during movement by fluorescence microscopy.
It is well known that myosin and actin play important roles as a motile apparatus in cells, and as a cytoskeleton of cell structure in non-muscle cells. The purpose of this study was simultaneously to demonstrate the intracellular dynamics of myosin and actin in human neutrophils during movement. We designed a double-fluorescence labelling procedure utilising fluorescein isothiocyanate and rhodamine. Myosin was labelled with the green-fluorescence, F-actin was labelled with the red-fluorescence and the coexisting myosin and F-actin were labelled with yellow-fluorescence. We obtained the dual image of myosin and F-actin in human neutrophils during movement by this procedure through a conventional fluorescence microscope. Dual image can interpret the relationship between myosin and actin in human neutrophils during movement, however, further studies are required to elucidate the contractile mechanism in such cells.
Takubo
T
T
Department of Clinical and Laboratory Medicine, Osaka City University Medical School, Japan.
Tatsumi
N
N
D000199:Actins / Q000097:blood*; D002465:Cell Movement; D006801:Humans; D008856:Microscopy, Fluorescence; D009218:Myosins / Q000097:blood*; D009504:Neutrophils / Q000737:chemistry / Q000502:physiology*
D000199:Actins; D009218:Myosins