Collaborative study to assess the suitability of a proposed working reagent for human parvovirus B19 DNA detection in plasma pools by gene amplification techniques. B19 Collaborative Study Group.
OBJECTIVE
A collaborative study was done to examine the sensitivity and specificity of assays for the detection of human parvovirus B19 DNA in plasma pools by PCR techniques and to establish a working reagent for B19 DNA testing of plasma pools.


METHODS
Duplicate samples consisting of a tenfold dilution series of a positive cryosupernatant diluted in B19 DNA-negative cryosupernatant were sent to 17 laboratories.


RESULTS
The sensitivity of the assays varied: 2 laboratories were able to detect the 10(-7) dilution while 1 laboratory failed to detect B19 DNA in any samples. In addition, 5 laboratories obtained false-positive results.


CONCLUSIONS
In general, laboratories using assays optimised for rapid detection of B19 DNA in serum samples did not perform well, indicating that such rapid methods are not adequate for examination of plasma pools. The 10(-6) dilution was detected by approximately half the laboratories and could be used as the working reagent.
Saldanha
J
J
Division of Virology, NIBSC, South Mimms, UK. jsaldanha@nibsc.ac.uk
Minor
P
P
D004279:DNA, Viral / Q000097:blood / Q000302:isolation & purification*; D005069:Evaluation Studies as Topic; D006801:Humans; D007202:Indicators and Reagents; D016732:Parvovirus B19, Human / Q000302:isolation & purification*; D010949:Plasma / Q000821:virology*; D016133:Polymerase Chain Reaction; D011237:Predictive Value of Tests; D012680:Sensitivity and Specificity
D004279:DNA, Viral; D007202:Indicators and Reagents