A reevaluation of substrate specificity of the rat cation transporter rOCT1.
The substrate specificity of the previously cloned rat cation transporter rOCT1, which is expressed in kidney, liver, and small intestine, was reevaluated. rOCT1 is the first member of a new protein family comprising electrogenic and polyspecific cation transporters that transport hydrophilic cations like tetraethylammonium, choline, and monoamine neurotransmitters. Previous electrical measurements suggested that cations like quinine, quinidine, and cyanine 863, which have been classified as type 2 cations in the liver, are also transported by rOCT1, since they may induce inward currents in rOCT1 expressing Xenopus oocytes (Busch, A. E., Quester, S., Ulzheimer, J. C., Waldegger, S., Gorboulev, V., Arndt, P., Lang, F., and Koepsell, H. (1996) J. Biol. Chem. 271, 32599-32604). Tracer flux measurements with oocytes and with stably transfected human embryonic kidney cells showed that [3H]quinine and [3H]quinidine are not transported by rOCT1. The voltage dependence observed for the quinine- or quinidine-induced inward currents in rOCT1-expressing oocytes, and tracer efflux measurements indicate that the inward currents by type 2 cations are generated by the inhibition of electrogenic efflux of transported type 1 cations. Therefore, rOCT1 cannot contribute to transport of type 2 cations in the liver and the hepatic transporter for type 2 cations remains to be identified.
Nagel
G
G
Max-Planck-Insitiut für Biophysik, Kennedy-Allee 70, D-60596 Frankfurt, Germany. anat010@rzbox.uni-wuerzburg.de
Volk
C
C
Friedrich
T
T
Ulzheimer
J C
JC
Bamberg
E
E
Koepsell
H
H
D000818:Animals; D002352:Carrier Proteins / Q000378:metabolism* / Q000502:physiology; D002460:Cell Line; D005069:Evaluation Studies as Topic; D006801:Humans; D015640:Ion Channel Gating / Q000502:physiology; D017136:Ion Transport; D008564:Membrane Potentials; D008565:Membrane Proteins / Q000378:metabolism* / Q000502:physiology; D009865:Oocytes / Q000378:metabolism / Q000502:physiology; D027702:Organic Cation Transporter 1; D051381:Rats; D011994:Recombinant Proteins / Q000378:metabolism; D013379:Substrate Specificity; D014981:Xenopus
D002352:Carrier Proteins; D008565:Membrane Proteins; D027702:Organic Cation Transporter 1; D011994:Recombinant Proteins