Phase transition between hexagonal II (H[II]) and liquid-crystalline phase induced by interaction between solvents and segments of the membrane surface of dioleoylphosphatidylethanolamine.
We have investigated effects of several water-soluble organic solvents such as acetone, acetonitrile, and ethanol, which also have high solubility in alkane, on the structure and phase behavior of dioleoylphosphatidylethanolamine (DOPE) dispersion. X-ray diffraction data indicated that a phase transition from hexagonal II (H[II]) to liquid-crystalline (L alpha) phase in DOPE dispersion, occurred at 13% (v/v) acetone in H2O at 20 degrees C. The temperature of the L alpha-H(II) phase transition of DOPE dispersion increased with an increase in acetone concentration, and it was 37 degrees C at 20% (v/v) acetone. These results indicated that acetone stabilized L alpha phase relative to H(II) phase. Similar results were obtained in interactions of DOPE dispersions in H2O with acetonitrile or ethanol. X-ray diffraction data indicated that the H(II)-L alpha phase transition occurred at 9.0% (v/v) acetonitrile or at 9.8% (v/v) ethanol in water at 20 degrees C. The L alpha-H(II) phase transition temperature of DOPE dispersion increased with an increase in acetonitrile or ethanol concentration, and it was 66 degrees C at 20% (v/v) acetonitrile. Substitution of H2O by D2O (deuterium oxide) increased their threshold concentrations of the H(II)-L alpha phase transition induced by these organic solvents. A mechanism of these phase transitions and the effect of the substitution of H2O by D2O is proposed and discussed; an interaction free energy between solvents and the hydrophobic segments of the alkyl chains in the membrane surface, and also a packing parameter of the phospholipid may be main factors to explain these phenomena reasonably.
Kinoshita
K
K
Department of Physics, Faculty of Science, Shizuoka University, Japan.
Yamazaki
M
M
D000096:Acetone; D000097:Acetonitriles; D002152:Calorimetry, Differential Scanning; D017666:Deuterium Oxide; D000431:Ethanol; D008561:Membrane Fusion; D008567:Membranes, Artificial; D008958:Models, Molecular; D010714:Phosphatidylethanolamines / Q000737:chemistry*; D012997:Solvents; D014961:X-Ray Diffraction
D000097:Acetonitriles; D008567:Membranes, Artificial; D010714:Phosphatidylethanolamines; D012997:Solvents; D000096:Acetone; C094877:dioleoyl phosphatidylethanolamine; D000431:Ethanol; D017666:Deuterium Oxide; C032159:acetonitrile