title: Ion-molecule reactions as probes of gas-phase structures of peptides and proteins.
abstract: A review with over 100 references describes the recent applications of ion-molecule reactions to the study of gas-phase protonated peptides and proteins. The topic is focused specifically on the proton transfer and hydrogen-deuterium exchange reactions of amino acids, peptides, and proteins. A brief background is given of the various methods used for assigning proton affinities and gas-phase basicities. The methods used for measuring the kinetics of deuterium incorporation of charged ion in the presence of a background pressure of deuterating reagents are also described. Ion-molecule reactions are used to determine, among other things, the gas-phase basicities and proton affinities of amino acids, peptides, and proteins, the sites of protonation, intra- and intermolecular interactions, and conformational differences and changes in gas-phase ionic species. Singly charged and multiply charged ions are both covered.
authors.affiliation: Department of Chemistry, University of California, Davis 95616, USA.
authors.forename: M K
authors.initials: MK
authors.lastname: Green
authors.forename: C B
authors.initials: CB
authors.lastname: Lebrilla
mesh_terms: D005740:Gases / Q000737:chemistry*; D007477:Ions; D013058:Mass Spectrometry / Q000379:methods*; D015335:Molecular Probes; D010455:Peptides / Q000737:chemistry*; D011506:Proteins / Q000737:chemistry*; D013816:Thermodynamics
chemical_list: D005740:Gases; D007477:Ions; D015335:Molecular Probes; D010455:Peptides; D011506:Proteins