title: Immunization of cattle with a BHV1 vector vaccine or a DNA vaccine both coding for the G protein of BRSV.
abstract: A gE-negative bovine herpesvirus 1 (BHV1) vector vaccine carrying a gene coding for the G protein of bovine respiratory syncytial virus (BRSV) (BHV1/BRSV-G) induced the same high degree of protection in calves against BRSV infection and BHV1 infection as a multivalent commercial vaccine. A DNA plasmid vaccine, carrying the same gene as the BHV1/BRSV-G vaccine, significantly reduced BRSV shedding after BRSV infection compared with that in control calves, but less well than the BHV1/BRSV-G vaccine. Flow cytometric analysis showed a significant relative increase of gamma/delta+ T cells in peripheral blood after BRSV challenge-infection of the calves of the control group but not in the vaccinated groups. These results indicate that the G protein of BRSV can induce significant protection against BRSV infection in cattle, and that the BHV1/BRSV-G vaccine protects effectively against a subsequent BRSV and BHV1 infection.
authors.affiliation: Institute for Animal Science and Health (ID-DLO), Department of Mammalian Virology, Lelystad, Netherlands.
authors.forename: R S
authors.initials: RS
authors.lastname: Schrijver
authors.forename: J P
authors.initials: JP
authors.lastname: Langedijk
authors.forename: G M
authors.initials: GM
authors.lastname: Keil
authors.forename: W G
authors.initials: WG
authors.lastname: Middel
authors.forename: M
authors.initials: M
authors.lastname: Maris-Veldhuis
authors.forename: J T
authors.initials: JT
authors.lastname: Van Oirschot
authors.forename: F A
authors.initials: FA
authors.lastname: Rijsewijk
mesh_terms: D000818:Animals; D000914:Antibodies, Viral / Q000276:immunology; D002417:Cattle; D002418:Cattle Diseases / Q000276:immunology / Q000517:prevention & control*; D006566:Herpesviridae Infections / Q000662:veterinary*; D007242:Herpesvirus 1, Bovine / Q000235:genetics / Q000276:immunology*; D018357:Respiratory Syncytial Virus Infections / Q000276:immunology / Q000662:veterinary*; D018112:Respiratory Syncytial Virus, Bovine / Q000235:genetics* / Q000276:immunology; D016176:T-Lymphocyte Subsets / Q000276:immunology; D014611:Vaccination / Q000662:veterinary*; D017778:Vaccines, Combined / Q000235:genetics / Q000276:immunology / Q000627:therapeutic use*; D019444:Vaccines, DNA / Q000235:genetics / Q000627:therapeutic use*; D014759:Viral Envelope Proteins / Q000235:genetics* / Q000276:immunology*; D014765:Viral Vaccines / Q000235:genetics / Q000276:immunology / Q000627:therapeutic use*
chemical_list: D000914:Antibodies, Viral; C066028:G protein, Bovine respiratory syncytial virus; D017778:Vaccines, Combined; D019444:Vaccines, DNA; D014759:Viral Envelope Proteins; D014765:Viral Vaccines