Inhibition of bacterial growth by synthetic SP-B1-78 peptides.
Residues 12-34 of mature human pulmonary surfactant protein B (SP-B1-78) are 68% homologous to residues 48-72 of the frog peptide antibiotic dermaseptin b I. We examined the effects of SP-B1-78 on the growth of Escherichia coli in order to find whether full length SP-B1-78 might act as a peptide antibiotic. We found that SP-B1-78 peptide inhibited growth of E. coli (MIC = 210 micrograms.ml-1), but that the SP-B variant [R/K-->S]SP-B1-78 was less potent (MIC = 500 micrograms.ml-1).
Kaser
M R
MR
Department of Pediatrics, University of California, San Francisco, USA. mkaser@sfghpeds.ucsf.edu
Skouteris
G G
GG
D000595:Amino Acid Sequence; D000704:Analysis of Variance; D004926:Escherichia coli / Q000187:drug effects* / Q000254:growth & development; D006131:Growth Inhibitors / Q000494:pharmacology*; D008969:Molecular Sequence Data; D010446:Peptide Fragments / Q000494:pharmacology*; D011510:Proteolipids / Q000737:chemistry / Q000494:pharmacology*; D011663:Pulmonary Surfactants / Q000737:chemistry / Q000494:pharmacology*
D006131:Growth Inhibitors; D010446:Peptide Fragments; D011510:Proteolipids; D011663:Pulmonary Surfactants