Virulence and colonization-associated properties of Helicobacter pylori isolated from children and adolescents.
Helicobacter pylori isolates from 32 children and adolescents were characterized with respect to putative virulence and colonization-associated properties. Only 3 of the subjects had duodenal ulcer. All but 2 of the remaining 29 had various degrees of chronic gastric inflammation. No significant correlation between degree of inflammation and presence of the cag-pathogenicity island, cytotoxin production, vacA alleles associated with cytotoxin expression, and binding ability to the Lewis(b) (Le[b]) oligosaccharide was found. Only 4 isolates expressed the Le(b)-specific adhesin, of which 3 were also cag region-positive. This is in contrast to adults with gastritis or peptic ulcer disease (or both), in whom most of the H. pylori isolates bind Le(b). In an in situ binding assay H. pylori were less able to adhere to gastric surface mucous cells in biopsies taken from children compared with adults, suggesting a lower expression of the Le(b) oligosaccharide in the children.
Celik
J
J
Swedish Institute of Infectious Disease Control, and Department of Women and Child Health, St. Göran's Children's Hospital, Stockholm.
Su
B
B
Tirén
U
U
Finkel
Y
Y
Thoresson
A C
AC
Engstrand
L
L
Sandstedt
B
B
Bernander
S
S
Normark
S
S
D000293:Adolescent; D000483:Alleles; D000942:Antigens, Bacterial; D000951:Antigens, Neoplasm / Q000235:genetics / Q000378:metabolism; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism; D002648:Child; D002675:Child, Preschool; D003603:Cytotoxins / Q000235:genetics / Q000378:metabolism; D004269:DNA, Bacterial / Q000235:genetics; D004381:Duodenal Ulcer / Q000382:microbiology; D005260:Female; D005756:Gastritis / Q000382:microbiology; D015870:Gene Expression; D005798:Genes, Bacterial; D006028:Glycosphingolipids / Q000235:genetics / Q000378:metabolism; D016481:Helicobacter Infections / Q000382:microbiology*; D016480:Helicobacter pylori / Q000378:metabolism / Q000472:pathogenicity*; D006801:Humans; D007150:Immunohistochemistry; D007983:Lewis Blood Group Antigens; D008297:Male; D016133:Polymerase Chain Reaction
D000942:Antigens, Bacterial; D000951:Antigens, Neoplasm; D001426:Bacterial Proteins; D003603:Cytotoxins; D004269:DNA, Bacterial; D006028:Glycosphingolipids; D007983:Lewis Blood Group Antigens; C074689:VacA protein, Helicobacter pylori; C080866:cagA protein, Helicobacter pylori; C072748:trifucosyl-Lewis-b antigen