title: Hemagglutination ability and adherence to the Buffalo green monkey kidney cell line of uropathogenic Escherichia coli.
abstract: The hemagglutination ability and adherence capacity to the Buffalo green monkey (BGM) kidney cell line of 160 wild-type strains of Escherichia coli isolated from bacteriuric patients were investigated. It was found that P-fimbriated E. coli strains adhered significantly better to BGM cells than did strains in which P-fimbriae were not detected, which is in accordance with the capacity of P-fimbriated strains to cause unobstructive pyelonephritis and with receptor distribution for P-fimbriae in the urinary tract. The strains which exhibited other adhesions, alone or simultaneously, showed reduced adherence to BGM cells, while non-agglutinating strains, mostly isolated from urine of patients with asymptomatic bacteriuria, did not adhere at all or adhered poorly to the utilized cell line. The BGM cells served as a good experimental model for investigation of uropathogenic E. coli adherence; because these cells originate from the upper urinary tract, they are viable and not coated with Tamm-Horsfall protein.
authors.affiliation: Department of Microbiology and Parasitology, Medical School, University of Zagreb, Croatia.
authors.forename: J
authors.initials: J
authors.lastname: Vranes
mesh_terms: D018829:Adhesins, Bacterial / Q000378:metabolism*; D000818:Animals; D001422:Bacterial Adhesion; D001437:Bacteriuria / Q000382:microbiology; D002478:Cells, Cultured; D002522:Chlorocebus aethiops; D003556:Cystitis / Q000382:microbiology; D004926:Escherichia coli / Q000472:pathogenicity*; D010861:Fimbriae, Bacterial / Q000378:metabolism; D006388:Hemagglutinins / Q000378:metabolism*; D011704:Pyelonephritis / Q000382:microbiology; D014552:Urinary Tract Infections / Q000382:microbiology*
chemical_list: D018829:Adhesins, Bacterial; D006388:Hemagglutinins