Effect of shaking of corneal endothelial preservation.
OBJECTIVE
To investigate the effect of shaking on corneal endothelial preservation.


METHODS
Thirty-six dog corneal buttons were obtained under standard eye bank procedure, after the animals had been sacrificed for cardiovascular experiments in the surgical department. They were equally divided into two groups. In Group I, buttons were put in Dexsol preservative medium and preserved at 4 degrees C. In Group II, buttons were put in Dexsol preservative medium and shaken in a shaking incubator at a speed of 5 rpm. at 4 degrees C for 10 h. After shaking, they were returned to a 4 degrees C refrigerator until examination. Three buttons from each group underwent specular microscopy, scanning electron microscopy (SEM), and alizarin red with trypan blue stain examinations on days 0, 1, 3, 5, 7 and 9, respectively.


RESULTS
In Group I, intercellular borders became blurred under specular microscopy, beginning on day 5. However, endothelial cell count did not change significantly until day 9. Intercellular digitations and wrinkling of intercellular borders were seen under alizarin red with trypan blue stain and SEM examination, beginning on day 3. Pleomorphism and ill-defined intercellular junctions were seen under SEM on day 7. There was no obvious denudement of the endothelial sheet, but a few scattered exfoliations were seen in Group I. In Group II, endothelial cell count did not decrease on day 1, but an endothelial image could not be obtained by specular microscopy 3 days after treatment. Alizarin red with trypan blue stain and SEM examination revealed that the endothelial cells were denuded from the Descemet's membrane three days after shaking. Severe stromal edema was seen under SEM five days after shaking.


CONCLUSIONS
The results showed that shaking had a detrimental effect on endothelial cell preservation. Vigorous shaking should be avoided during transportation of corneal buttons. It is advisable to perform penetrating keratoplasty as soon as possible upon receiving transported donor corneas.
Wang
I J
IJ
Department of Opthalmology, National Taiwan University Hospital, Taipei.
Hu
F R
FR
D000818:Animals; D000880:Anthraquinones; D002452:Cell Count; D002809:Chondroitin Sulfates; D004396:Coloring Agents; D015925:Cryopreservation; D016895:Culture Media, Serum-Free; D004285:Dogs; D004728:Endothelium, Corneal / Q000648:ultrastructure*; D006531:HEPES; D008855:Microscopy, Electron, Scanning; D009926:Organ Preservation; D009930:Organic Chemicals; D014186:Transportation; D014343:Trypan Blue; D014732:Vibration / Q000009:adverse effects*
D000880:Anthraquinones; D004396:Coloring Agents; D016895:Culture Media, Serum-Free; C075735:Dexsol; D009930:Organic Chemicals; C010078:alizarin; D002809:Chondroitin Sulfates; D014343:Trypan Blue; D006531:HEPES