Optimisation of the enantiomeric separation of 12 2-aminotetralin analogues using Chiral AGP high-performance liquid chromatography by simultaneous factorial design.
A method for the simultaneous optimisation of mobile phase composition for the resolution of pairs of enantiomers of 12 2-aminotetralin analogues is presented. The selectivity necessary to discriminate between 12 analytes was obtained by using mass selective detection. The ability to examine more than a few analytes at a time extends the otherwise limited applicability of a factorial design strategy to the rapid development of chiral assays.
Wood
S A
SA
Pharmacia and Upjohn, Crawley, West Sussex, UK.
Long
J M
JM
Simmonds
R J
RJ
Bridges
J W
JW
Stevenson
D
D
D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D013058:Mass Spectrometry / Q000379:methods*; D013764:Tetrahydronaphthalenes / Q000032:analysis / Q000737:chemistry* / Q000302:isolation & purification*
D013764:Tetrahydronaphthalenes; C006561:2-aminotetralin