title: Ciprofloxacin-resistant Escherichia coli emerging in a rehabilitation medical center.
abstract: A retrospective review of laboratory records from 1988 to 1996 has shown an increased rate of ciprofloxacin-resistant (cip(r)) Escherichia coli in our rehabilitation center. Resistance increased from 0.6% in 1989 to 5.9% in 1996. Of 7870 E. coli strains isolated during this period, 257 cip(r)-E. coli were recovered from 257 patients. The majority (96%) of these resistant strains were isolated from the urine samples. One hundred and twenty strains of cip(r)-E. coli were also resistant to four other fluoroquinolones. MICs ranging from 64 to 512 micrograms/mL were observed in 75% of the strains and > or = 1028 micrograms/mL in 6.4% of the strains. Resistance to ciprofloxacin was due to possible mutations in topoisomerase gyrA.
authors.affiliation: Department of Pathology, University of Southern California School of Medicine, Los Angeles, USA.
authors.forename: H N
authors.initials: HN
authors.lastname: Canawati
authors.forename: R
authors.initials: R
authors.lastname: el-Farra
authors.forename: J
authors.initials: J
authors.lastname: Seymour
authors.forename: J
authors.initials: J
authors.lastname: Shimashita
authors.forename: D
authors.initials: D
authors.lastname: Dunn
authors.forename: J Z
authors.initials: JZ
authors.lastname: Montgomerie
mesh_terms: D000890:Anti-Infective Agents / Q000494:pharmacology*; D001437:Bacteriuria / Q000188:drug therapy / Q000453:epidemiology / Q000382:microbiology; D002939:Ciprofloxacin / Q000494:pharmacology*; D027081:DNA Gyrase; D004250:DNA Topoisomerases, Type II / Q000235:genetics; D004352:Drug Resistance, Microbial; D004926:Escherichia coli / Q000187:drug effects* / Q000201:enzymology / Q000235:genetics; D004927:Escherichia coli Infections / Q000188:drug therapy* / Q000453:epidemiology / Q000382:microbiology*; D005798:Genes, Bacterial; D006801:Humans; D015141:Los Angeles / Q000453:epidemiology; D008826:Microbial Sensitivity Tests; D009154:Mutation; D012047:Rehabilitation Centers; D012189:Retrospective Studies; D013997:Time Factors
chemical_list: D000890:Anti-Infective Agents; D002939:Ciprofloxacin; D027081:DNA Gyrase; D004250:DNA Topoisomerases, Type II