Lack of effect of different cytokines on expression of membrane-bound regulators of complement activity on human uveal melanoma cells.
Tumor cells are protected from antibody-dependent complement-mediated lysis by membrane-bound regulators of complement activation (m-RCA). m-RCA are expressed on uveal melanoma cells. We determined whether cytokine treatment affects expression of m-RCA on these cells in vitro. m-RCA expression on uveal melanoma cell lines was studied by flow cytometry, using monoclonal antibodies directed against CD46, CD55, and CD59. Cytokines studied were interferon-alpha (IFN-alpha), IFN-gamma, interleukin-1B (IL-1B), IL-12, and tumor necrosis factor-alpha (TNF-alpha). All three m-RCA were expressed on the uveal melanoma cell lines (CD59>>CD46>CD55), although in variable amounts. With a few exceptions, the cytokines had no effect on m-RCA expression. CD55 expression was not influenced by any of the cytokines. IFN-gamma downregulated expression of CD46 on one cell line (p < 0.01). TNF-alpha upregulated CD59 expression on two of the five cell lines (p < 0.012 and p < 0.001, respectively), which effect was dose dependent. IFN-alpha, IFN-gamma, IL1-beta, IL12, and TNF-alpha had limited effects on m-RCA expression on uveal melanoma cells in vitro.
Blom
D J
DJ
Department of Ophthalmology, Leiden University Medical Center, The Netherlands.
Goslings
W R
WR
De Waard-Siebinga
I
I
Luyten
G P
GP
Claas
F H
FH
Gorter
A
A
Jager
M J
MJ
D000970:Antineoplastic Agents / Q000627:therapeutic use*; D003165:Complement System Proteins / Q000378:metabolism*; D016207:Cytokines / Q000627:therapeutic use*; D004354:Drug Screening Assays, Antitumor; D006801:Humans; D007372:Interferons / Q000627:therapeutic use; D007378:Interleukins / Q000627:therapeutic use; D008545:Melanoma / Q000188:drug therapy* / Q000378:metabolism; D008565:Membrane Proteins / Q000378:metabolism*; D014407:Tumor Cells, Cultured; D014409:Tumor Necrosis Factor-alpha / Q000627:therapeutic use; D014604:Uveal Neoplasms / Q000188:drug therapy* / Q000378:metabolism
D000970:Antineoplastic Agents; D016207:Cytokines; D007378:Interleukins; D008565:Membrane Proteins; D014409:Tumor Necrosis Factor-alpha; D003165:Complement System Proteins; D007372:Interferons