Elevated 4-hydroxynonenal in ventricular fluid in Alzheimer's disease.
4-Hydroxynonenal (4-HNE), an aldehyde by-product of the peroxidation of fatty acids, has been shown to have toxic properties for neurons in culture. In light of increasing evidence that oxidative stress contributes to the neurodegenerative process in Alzheimer's disease (AD), we quantified levels of free and protein-bound 4-HNE in the ventricular fluid from 19 AD subjects and 13 control subjects by high-pressure liquid chromatography and dot-blot immunoassay. Free 4-HNE levels were found to be significantly elevated in the ventricular fluid of AD subjects compared with control subjects (p = 0.0096). These results demonstrate increased lipid peroxidation in AD brain and suggest a role for 4-HNE in the neurodegenerative process.
Lovell
M A
MA
Department of Chemistry, Sanders-Brown Center on Aging, University of Kentucky, Lexington 40536-0230, USA.
Ehmann
W D
WD
Mattson
M P
MP
Markesbery
W R
WR
D000368:Aged; D000369:Aged, 80 and over; D000447:Aldehydes / Q000737:chemistry / Q000378:metabolism*; D000544:Alzheimer Disease / Q000378:metabolism*; D001826:Body Fluids / Q000737:chemistry*; D002552:Cerebral Ventricles / Q000737:chemistry / Q000378:metabolism*; D002851:Chromatography, High Pressure Liquid; D015853:Cysteine Proteinase Inhibitors / Q000737:chemistry / Q000378:metabolism*; D005260:Female; D006801:Humans; D015227:Lipid Peroxidation; D008297:Male; D008875:Middle Aged; D009419:Nerve Tissue Proteins / Q000378:metabolism
D000447:Aldehydes; D015853:Cysteine Proteinase Inhibitors; D009419:Nerve Tissue Proteins; C027576:4-hydroxy-2-nonenal