Ethanol and glutamate effects on intracellular magnesium.
Acutely dissociated rat brain cells were loaded with the magnesium-sensitive fluorescent dye furaptra. Stimulation with varying concentrations of GLU + 1 microM GLY produced a concentration-dependent increase in free intracellular magnesium ([Mg2+]i) that was not dependent on the presence of extracellular Mg2+. Ethanol (50 mM) did not significantly inhibit GLU/GLY-stimulated increases in [Mg2+]i. However, ethanol alone significantly increased baseline [Mg2+]i.
Wilson
W R
WR
Division of Pharmacology and Toxicology, College of Pharmacy, University of Texas at Austin 78712, USA. walteroy@uts.cc.utexas.edu
Phillips
K S
KS
Leslie
S W
SW
D000818:Animals; D000831:Animals, Newborn; D001921:Brain / Q000187:drug effects* / Q000378:metabolism*; D002021:Buffers; D004305:Dose-Response Relationship, Drug; D000431:Ethanol / Q000008:administration & dosage / Q000494:pharmacology*; D005456:Fluorescent Dyes; D016257:Fura-2 / Q000031:analogs & derivatives; D018698:Glutamic Acid / Q000008:administration & dosage / Q000494:pharmacology*; D005998:Glycine / Q000008:administration & dosage / Q000494:pharmacology; D007552:Isotonic Solutions; D008274:Magnesium / Q000378:metabolism*; D051381:Rats
D002021:Buffers; D005456:Fluorescent Dyes; D007552:Isotonic Solutions; C051865:Krebs-Ringer solution; C058776:2-(2-(5-carboxy)oxazole)-5-hydroxy-6-aminobenzofuran-N,N,O-triacetic acid; D000431:Ethanol; D018698:Glutamic Acid; D008274:Magnesium; D005998:Glycine; D016257:Fura-2