Sulpiride-induced increases in serum prolactin levels in female rats exposed prenatally to alcohol.
We examined the impact of prenatal alcohol exposure on serum prolactin levels and on the ability of the D2 dopamine antagonist sulpiride to stimulate prolactin release in Long-Evans rats. Pregnant rats were intubated with alcohol (0, 3, or 5 g/kg/day) from gestational day 8 (GD8) to GD20. Adult female offspring were screened for estrous cycle stage. At diestrus, the rats were challenged with a single dose of sulpiride (0, 10, or 40 micrograms/kg) and trunk blood was collected 20 min later. After prenatal exposure to either dose of alcohol, mean basal serum levels of prolactin were about 65% less than the 0 g/kg group, and the 35-40% mean differences from an untreated control group were not significant. Sulpiride produced dramatic dose-dependent increases in serum prolactin levels in all prenatal treatment groups. Across all doses of sulpiride, the group given the higher dose of prenatal alcohol (5 g/kg/day) had significantly lower serum prolactin levels than all other groups. There was no significant interaction between prenatal treatment and sulpiride dose. Neither prenatal alcohol exposure nor sulpiride injections had significant effects on serum corticosterone levels in this study. Although the current results are unclear regarding a baseline decrease in prolactin levels after prenatal alcohol exposure, the overall results suggest that prenatal alcohol exposure decreases prolactin levels but there is no evidence that it does so by altering dopaminergic tone in hypothalamus of female rats.
Hannigan
J H
JH
Department of Obstetrics & Gynecology, C. S. Mott Center for Human Growth and Development, Wayne State University School of Medicine, Detroit, MI 48201, USA. jhannig@cms.cc.wayne.edu
Hackett
J A
JA
Tilak
J
J
Subramanian
M G
MG
D000818:Animals; D001724:Birth Weight; D003345:Corticosterone / Q000097:blood; D018492:Dopamine Antagonists / Q000494:pharmacology*; D000431:Ethanol / Q000633:toxicity*; D005260:Female; D008098:Litter Size; D008297:Male; D011247:Pregnancy; D011297:Prenatal Exposure Delayed Effects; D011388:Prolactin / Q000097:blood*; D051381:Rats; D013469:Sulpiride / Q000494:pharmacology*
D018492:Dopamine Antagonists; D000431:Ethanol; D013469:Sulpiride; D011388:Prolactin; D003345:Corticosterone