Four-stranded DNA formed by isoguanine quartets: complex stoichiometry, thermal stability and resistance against exonucleases.
Single stranded DNA-fragments containing short runs of isoguanine such as d(T4iG4T4) (5) or d(iG4T4) (6) form quartet structures by self-assembly of the isoguanine residues. The stoichiometry of the complexes is deduced from mixed aggregates formed between d(T4iG4T4) and d(iG4T4). The iGd-tetrads are more stable with regard to their thermal denaturation and to their resistance against enzymatic phosphodiester hydrolysis than those formed by dG.
Seela
F
F
Laboratorium für Organische und Bioorganische Chemie, Universität Osnabrück, Germany.
Wei
C
C
Melenewski
A
A
D001483:Base Sequence; D002942:Circular Dichroism; D004247:DNA / Q000737:chemistry*; D004355:Drug Stability; D005090:Exodeoxyribonucleases; D006147:Guanine; D006358:Hot Temperature; D046911:Macromolecular Substances; D008958:Models, Molecular; D008961:Models, Structural; D009690:Nucleic Acid Conformation; D009691:Nucleic Acid Denaturation; D009838:Oligodeoxyribonucleotides / Q000737:chemistry*; D013816:Thermodynamics
D046911:Macromolecular Substances; D009838:Oligodeoxyribonucleotides; D006147:Guanine; D004247:DNA; C008183:isoguanine; D005090:Exodeoxyribonucleases