title: Protein phosphorylation in snail cardiocytes stimulated with molluscan peptide SCPb.
abstract: Dissociated muscle cells prepared from hearts of the pulmonate snail Helix aspersa were used to study signal transduction induced by molluscan cardioactive peptides. The effects of SCPb on the cAMP levels of whole hearts and the cell preparation were compared. The cells responded to SCPb with a dose-dependent increase in cAMP that had the same structure-activity relations as seen in the intact tissue. SCPb increased the phosphorylation of a 53 kDa protein in a dose dependent manner; threshold was 10(-9) M. The SCPb-induced phosphorylation was mimicked by forskolin and 8-CPT-cAMP. FMRFamide stimulation had no effect on the phosphorylation of this protein.
authors.affiliation: Whitney Laboratory, University of Florida, St. Augustine 32086, USA. gere@aug.com
authors.forename: G
authors.initials: G
authors.lastname: Reich
authors.forename: K E
authors.initials: KE
authors.lastname: Doble
authors.forename: M J
authors.initials: MJ
authors.lastname: Greenberg
mesh_terms: D000818:Animals; D001048:Aplysia; D006372:Helix, Snails / Q000187:drug effects* / Q000378:metabolism; D066298:In Vitro Techniques; D007447:Invertebrate Hormones / Q000494:pharmacology*; D009206:Myocardium / Q000166:cytology / Q000378:metabolism*; D009479:Neuropeptides / Q000494:pharmacology*; D010766:Phosphorylation; D011506:Proteins / Q000378:metabolism*; D013268:Stimulation, Chemical
chemical_list: D007447:Invertebrate Hormones; D009479:Neuropeptides; D011506:Proteins; C041307:small cardioactive peptide B