Characterization of room temperature induced apoptosis in HL-60.
We found that exposure to room temperature (RT/21 degrees C) causes apoptosis in HL-60 cells. Here we characterized RT-induced apoptosis in HL-60. After exposure to RT, apoptosis starts within 6 h and more than 80% of the cells underwent apoptosis within 20 h. All cells, however, were committed to apoptosis after 16 h and no viable cells could be recovered. The caspase-1 inhibitor (YVAD-CHO) effectively blocked apoptosis, whereas the caspase-3 inhibitor (DEVD-CHO) did not. About 20% of newly obtained early passage HL-60 cells (passage 10) also underwent apoptosis by RT treatment. These data suggest that some population in HL-60 which responds to RT with apoptosis became dominant during passaging.
Shimura
M
M
Department of Haematology, International Medical Center of Japan, Tokyo.
Ishizaka
Y
Y
Yuo
A
A
Hatake
K
K
Oshima
M
M
Sasaki
T
T
Takaku
F
F
D017209:Apoptosis / Q000187:drug effects / Q000502:physiology*; D020170:Caspase 1; D053148:Caspase 3; D020169:Caspases; D003546:Cysteine Endopeptidases / Q000378:metabolism; D015853:Cysteine Proteinase Inhibitors / Q000494:pharmacology*; D005047:Etoposide / Q000494:pharmacology; D018922:HL-60 Cells / Q000166:cytology / Q000502:physiology*; D006801:Humans; D015759:Ionomycin / Q000494:pharmacology; D007700:Kinetics; D009842:Oligopeptides / Q000494:pharmacology; D013696:Temperature; D013997:Time Factors
D015853:Cysteine Proteinase Inhibitors; D009842:Oligopeptides; C108637:aspartyl-glutamyl-valyl-aspartal; C096429:L 709049; D015759:Ionomycin; D005047:Etoposide; C505407:CASP3 protein, human; D053148:Caspase 3; D020169:Caspases; D003546:Cysteine Endopeptidases; D020170:Caspase 1