Determination of fast proton exchange rates of biomolecules by NMR using water selective diffusion experiments.
We present a new water selective pulse sequence allowing rapid determination of exchange rates of labile protons on the millisecond time scale. Using diffusion measurements, exchange rates of resolved protons can be determined without prior knowledge of relaxation parameters in a short overnight experiment. The use of a sensitive, highly selective and easy to implement water excitation scheme allows for its straightforward application to a wide range of biomolecules. The results obtained for the imino proton exchange rates of a 16 bp DNA are in strong agreement with values obtained by the classical approach of two-dimensional exchange spectroscopy.
Böckmann
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A
Laboratoire de RMN, ICSN-CNRS, Gif sur Yvette, France. bockmann@chem.columbia.edu
Guittet
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E
D001483:Base Sequence; D004247:DNA / Q000737:chemistry*; D004058:Diffusion; D008956:Models, Chemical; D019906:Nuclear Magnetic Resonance, Biomolecular / Q000379:methods; D009690:Nucleic Acid Conformation; D009838:Oligodeoxyribonucleotides / Q000737:chemistry*; D011522:Protons; D012680:Sensitivity and Specificity; D014867:Water
D009838:Oligodeoxyribonucleotides; D011522:Protons; D014867:Water; D004247:DNA