Modeling of in vivo proteolytic degradation of hemoglobin.
Based on the amino acid sequences of endogenous peptides and X-ray spatial structure, mechanism of the in vivo proteolly degradation of bovine hemoglobin was analysed. The degradation was shown to be a multi-stage process. Its first stage is determined by the spatial organization of the native protein substrate, and the next stages-by the distribution of the electrostatic field potential of the protein fragments formed at the earlier stage.
Nekrasov
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AN
Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Moscow, Russia. alne@ibch.siobc.ras.ru
Lexikova
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EE
Karelin
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D000595:Amino Acid Sequence; D000818:Animals; D002417:Cattle; D003198:Computer Simulation; D018360:Crystallography, X-Ray; D006454:Hemoglobins / Q000737:chemistry* / Q000378:metabolism*; D008958:Models, Molecular; D008969:Molecular Sequence Data; D010446:Peptide Fragments / Q000737:chemistry; D017434:Protein Structure, Tertiary; D055672:Static Electricity
D006454:Hemoglobins; D010446:Peptide Fragments