title: Detection of glycosaminoglycans as a copper(II) complex in high-performance liquid chromatography.
abstract: Glycosaminoglycans including heparin, heparan sulfate, chondroitin sulfate, dermatan sulfate and hyaluronan were analyzed by high-performance gel filtration chromatography. Detection was achieved at 240 nm based on the formation of copper(II) complex in copper sulfate solution at low pH. Detection of the copper(II)-heparin complex is sensitive, permitting the analysis of as little as 10(-7) g. This method was also successfully applied to the analysis of the chemically derivatized glycosaminoglycans (de-N-acetylated and/or oversulfated chondroitin sulfate) that cannot be depolymerized by enzymes such as heparin and chondroitin lyases.
authors.affiliation: Faculty of Pharmaceutical Sciences, Chiba University, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Toida
authors.forename: M
authors.initials: M
authors.lastname: Shima
authors.forename: S
authors.initials: S
authors.lastname: Azumaya
authors.forename: T
authors.initials: T
authors.lastname: Maruyama
authors.forename: H
authors.initials: H
authors.lastname: Toyoda
authors.forename: T
authors.initials: T
authors.lastname: Imanari
authors.forename: R J
authors.initials: RJ
authors.lastname: Linhardt
mesh_terms: D002614:Chelating Agents / Q000032:analysis* / Q000737:chemistry; D002851:Chromatography, High Pressure Liquid; D003300:Copper / Q000737:chemistry*; D006025:Glycosaminoglycans / Q000032:analysis* / Q000737:chemistry; D006863:Hydrogen-Ion Concentration; D012680:Sensitivity and Specificity; D013056:Spectrophotometry, Ultraviolet
chemical_list: D002614:Chelating Agents; D006025:Glycosaminoglycans; D003300:Copper