Truncations of the C-terminal cytoplasmic domain of MG160, a medial Golgi sialoglycoprotein, result in its partial transport to the plasma membrane and filopodia.
MG160, a type I cysteine-rich membrane sialoglycoprotein residing in the medial cisternae of the rat Golgi apparatus, is highly homologous to CFR, a fibroblast growth factor receptor, and ESL-1, an E-selectin ligand located at the cell surface of mouse myeloid cells and recently detected in the Golgi apparatus as well. The mechanism for the transport of MG160 from the Golgi apparatus to the cell surface is unknown. In this study we found that differential processing of the carboxy-terminal cytoplasmic domain (CD), consisting of amino acids Arg1159 Ile Thr Lys Arg Val Thr Arg Glu Leu Lys Asp Arg1171, resulted in the partial transport of the protein to the plasma membrane and filopodia. In Chinese hamster ovary cells (CHO), stably transfected with the entire cDNA encoding MG160, the protein was localized in the Golgi apparatus. However, when the terminal Arg1171 or up to nine distal amino acids were deleted, the protein was distributed to the plasma membrane and filopodia as well as the Golgi apparatus. This report shows that the CD of an endogenous type I Golgi protein is important for its efficient retention and identifies a unique residue preference in this process. Cleavage within the CD of MG160 may constitute a regulatory mechanism for the partial export of the protein from the Golgi apparatus to the plasma membrane and filopodia.
Gonatas
J O
JO
Department of Pathology, University of Pennsylvania School of Medicine, Philadelphia, PA 19104, USA. Gonatas@pobox.upenn.edu
Chen
Y J
YJ
Stieber
A
A
Mourelatos
Z
Z
Gonatas
N K
NK
D000596:Amino Acids; D000818:Animals; D000906:Antibodies / Q000378:metabolism; D001120:Arginine; D001665:Binding Sites; D001692:Biological Transport; D001710:Biotin / Q000031:analogs & derivatives; D016466:CHO Cells; D002462:Cell Membrane / Q000378:metabolism; D006224:Cricetinae; D003470:Culture Media; D003593:Cytoplasm / Q000378:metabolism; D005260:Female; D006056:Golgi Apparatus / Q000378:metabolism*; D006801:Humans; D007124:Immunoenzyme Techniques; D016253:Microscopy, Immunoelectron; D011233:Precipitin Tests; D011499:Protein Processing, Post-Translational; D011817:Rabbits; D051381:Rats; D011956:Receptors, Cell Surface; D017468:Receptors, Fibroblast Growth Factor; D017384:Sequence Deletion; D012795:Sialoglycoproteins / Q000096:biosynthesis / Q000235:genetics / Q000378:metabolism*; D013329:Structure-Activity Relationship; D013388:Succinimides; D014162:Transfection
D000596:Amino Acids; D000906:Antibodies; D003470:Culture Media; D011956:Receptors, Cell Surface; D017468:Receptors, Fibroblast Growth Factor; D012795:Sialoglycoproteins; D013388:Succinimides; C086711:cysteine-rich fibroblast growth factor receptor; C061443:sulfosuccinimidyl 6-(biotinamido)hexanoate; D001710:Biotin; D001120:Arginine