title: Maintenance and down-regulation of primed neutrophil chemiluminescence activity in human whole blood.
abstract: Priming of polymorphonuclear neutrophils (PMN) in whole blood (by tumor necrosis factor alpha and interleukin-8 for enhancement of luminol-dependent chemiluminescence induced by human complement-opsonized zymosan) was stable for 120 min. In contrast, priming of isolated PMN in plasma-free suspension for responses to opsonized zymosan, formyl-methionyl-leucyl-phenylalanine, and phorbol myristate acetate was markedly less stable. Decay of priming was not due to irreversible inactivation of the terminal CL production machinery because PMN could be reprimed by platelet-activating factor or leukotriene B4. The tumor necrosis factor-alpha-primed state of isolated PMN was stabilized by host plasma in a concentration-dependent fashion. We conclude that PMN priming results in a dynamic state that is reversible. Our findings suggest the existence of blood-borne components that may act to stabilize or modify PMN priming.
authors.affiliation: Department of Surgery, Beth Israel Deaconess Medical Center and Harvard Medical School, Boston, Massachusetts 02215, USA.
authors.forename: G E
authors.initials: GE
authors.lastname: Brown
authors.forename: J
authors.initials: J
authors.lastname: Reiff
authors.forename: R C
authors.initials: RC
authors.lastname: Allen
authors.forename: G M
authors.initials: GM
authors.lastname: Silver
authors.forename: M P
authors.initials: MP
authors.lastname: Fink
mesh_terms: D015536:Down-Regulation; D006801:Humans; D016209:Interleukin-8 / Q000494:pharmacology; D008163:Luminescent Measurements; D009504:Neutrophils / Q000187:drug effects / Q000378:metabolism*; D016897:Respiratory Burst; D014409:Tumor Necrosis Factor-alpha / Q000494:pharmacology
chemical_list: D016209:Interleukin-8; D014409:Tumor Necrosis Factor-alpha