title: Study of the polymorphism of ovine alpha s1- and alpha s2-caseins by capillary electrophoresis.
abstract: Polymorphism of ovine alpha s-caseins was studied by capillary electrophoresis at pH 3.0 +/- 0.1. Individual caseins (CN) were selected according to their genetic variants, as determined by PAGE and isoelectric focusing. The ovine caseins, containing different genetic variants of alpha s1-CN and alpha s2-CN, were fractionated by cation-exchange FPLC. The alpha s1-CN variants A, B and C, and the fast moving alpha s2-CN variant were identified by a capillary electrophoresis method. The fast moving alpha s2-CN variant, so-called for its behaviour in PAGE, also had a faster electrophoretic mobility than the common alpha s2-CN when analysed by the present technique. The capillary electrophoresis method gave excellent, rapid, automated separation of alpha s1-CN and alpha s2-CN variants and was suitable for screening studies.
authors.affiliation: Instituto de Fermentaciones Industriales (CSIC), Madrid, España.
authors.forename: I
authors.initials: I
authors.lastname: Recio
authors.forename: M
authors.initials: M
authors.lastname: Ramos
authors.forename: L
authors.initials: L
authors.lastname: Amigo
mesh_terms: D000818:Animals; D002364:Caseins / Q000235:genetics* / Q000302:isolation & purification; D002851:Chromatography, High Pressure Liquid; D019075:Electrophoresis, Capillary; D004591:Electrophoresis, Polyacrylamide Gel; D014644:Genetic Variation; D007525:Isoelectric Focusing; D011110:Polymorphism, Genetic; D012756:Sheep / Q000235:genetics*
chemical_list: D002364:Caseins