title: Immunohistochemical and RT-PCR detection of Na+-dependent inorganic phosphate cotransporter (NaPi-2) in rat brain.
abstract: Expression of a renal Na+-dependent inorganic phosphate (Pi) cotransporter (NaPi-2) was studied in rat forebrain with reverse transcription and polymerase chain reaction (RT-PCR) and immunohistochemistry. RT-PCR analysis for total RNA from whole brain and sequencing of the PCR products showed expression of NaPi-2 mRNA in the brain. Immunohistochemical analysis revealed NaPi-2 staining in many nonpyramidal neurons of all six layers throughout neocortical areas and in neurons of proisocortical and periallocortical areas. NaPi-2-immunoreactive neurons were also detectable in the piriform cortex, hippocampal formation, caudate-putamen, amygdaloid nuclei and lateral geniculate nucleus. Furthermore, NaPi-2 staining was shown in ependymal cells and microvascular endothelial cells. The present results suggest that NaPi-2 is synthesized within the brain and involved in maintaining Pi homeostasis of certain neurons and/or the entire brain.
authors.affiliation: Department of Anatomy, School of Medicine, University of Tokushima, Japan. hisano@basic.med.tokushima-u.ac.jp
authors.forename: S
authors.initials: S
authors.lastname: Hisano
authors.forename: H
authors.initials: H
authors.lastname: Haga
authors.forename: Z
authors.initials: Z
authors.lastname: Li
authors.forename: S
authors.initials: S
authors.lastname: Tatsumi
authors.forename: K I
authors.initials: KI
authors.lastname: Miyamoto
authors.forename: E
authors.initials: E
authors.lastname: Takeda
authors.forename: Y
authors.initials: Y
authors.lastname: Fukui
mesh_terms: D000818:Animals; D001921:Brain / Q000166:cytology / Q000378:metabolism*; D002352:Carrier Proteins / Q000378:metabolism*; D004027:Diencephalon / Q000378:metabolism; D007150:Immunohistochemistry; D008297:Male; D009419:Nerve Tissue Proteins / Q000378:metabolism*; D009474:Neurons / Q000378:metabolism; D010710:Phosphates / Q000378:metabolism*; D016133:Polymerase Chain Reaction / Q000379:methods*; D051381:Rats; D017208:Rats, Wistar; D012964:Sodium / Q000502:physiology*; D013687:Telencephalon / Q000378:metabolism; D014158:Transcription, Genetic
chemical_list: D002352:Carrier Proteins; D009419:Nerve Tissue Proteins; D010710:Phosphates; D012964:Sodium