title: In vitro production of human antigen presenting cells issued from bone marrow of patients with cancer.
abstract: In the prospect of producing autologous antigen presenting cells (APC) to actively immunize patients with cancer against their own tumor we were interested in the in vitro generation of MC and/or dendritic cells. We observed that the best yielding in CD14+ cells was obtained by adding SCF and GM-CSF into RPMI 1640 completed medium and by using Teflon bags as culture-containers. The others growth factors tested (LIF, IL3 and M-CSF) were useless in term of production of macrophages. After a month of culture we usually obtained an average of 80% of CD14, CD33, CD64, CD11a, CD11b, CD11c and HLA-DR positive cells expressing the MGG staining phenotype of MC. For DC the best association of growth factors combined GM-CSF, IL-4 and SCF. Hence we could obtained at least 60% of CD1a+, CD14-, CD54+, CD58+, CD80+ and HLA DR+ dendritic cells.
authors.affiliation: Laboratoire d'Immunologie, Institut Bergonié, Centre Régional de Lutte Contre le Cancer, Bordeaux, France.
authors.forename: V
authors.initials: V
authors.lastname: Coulon
authors.forename: A
authors.initials: A
authors.lastname: Ravaud
authors.forename: S
authors.initials: S
authors.lastname: Huet
authors.forename: N
authors.initials: N
authors.lastname: Gualde
mesh_terms: D000938:Antigen-Presenting Cells / Q000276:immunology*; D002478:Cells, Cultured; D003713:Dendritic Cells / Q000166:cytology; D016178:Granulocyte-Macrophage Colony-Stimulating Factor / Q000494:pharmacology; D006412:Hematopoietic Stem Cells / Q000276:immunology*; D006801:Humans; D016130:Immunophenotyping; D010969:Plastics; D011138:Polytetrafluoroethylene; D019089:Stem Cell Factor / Q000494:pharmacology
chemical_list: D010969:Plastics; D019089:Stem Cell Factor; D016178:Granulocyte-Macrophage Colony-Stimulating Factor; D011138:Polytetrafluoroethylene