title: A simple and objective approach to identifying human round spermatids.
abstract: Although round spermatids have been studied extensively using staining techniques and electron microscopy, little information is available about their appearance in living conditions. We describe a method of collecting and identifying round spermatids from ejaculates and testicular biopsies. The validity of the selection procedure was confirmed by fluorescence in-situ hybridization. Based on cell size, morphological characteristics of nucleus and cytoplasm, and on the nucleus/cytoplasm ratio, we harvested a population of cells that was 84% haploid. This procedure can be applied to select spermatids for clinical or research purposes.
authors.affiliation: Department of Obstetrics and Gynecology, New York University Medical Center, NY 10016, USA.
authors.forename: T
authors.initials: T
authors.lastname: Angelopoulos
authors.forename: L
authors.initials: L
authors.lastname: Krey
authors.forename: A
authors.initials: A
authors.lastname: McCullough
authors.forename: A
authors.initials: A
authors.lastname: Adler
authors.forename: J A
authors.initials: JA
authors.lastname: Grifo
mesh_terms: D001706:Biopsy; D002467:Cell Nucleus / Q000648:ultrastructure; D002469:Cell Separation / Q000379:methods; D002499:Centrifugation, Density Gradient; D003593:Cytoplasm / Q000648:ultrastructure; D006238:Haploidy; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D008297:Male; D008853:Microscopy / Q000379:methods; D013087:Spermatids / Q000648:ultrastructure*; D013194:Staining and Labeling; D013737:Testis / Q000166:cytology