title: Quantification of conserved antigens in Helicobacter pylori during different culture conditions.
abstract: In this study, we raised monoclonal antibodies (MAbs) against three conserved Helicobacter pylori antigens, i.e., the N-acetylneuraminyllactose-binding fibrillar hemagglutinin, HpaA; the flagellin subunits, FlaA and FlaB; and a species-specific 26-kDa protein. The MAbs were used for the development of sensitive inhibition enzyme-linked immunosorbent assays for quantification of these antigens in H. pylori during various culture conditions. The quantities of these antigens varied considerably (up to 8-fold) during different culture procedures and between strains (up to 10-fold).
authors.affiliation: Department of Medical Microbiology and Immunology, Göteborg University, Sweden.
authors.forename: C
authors.initials: C
authors.lastname: Lindholm
authors.forename: J
authors.initials: J
authors.lastname: Osek
authors.forename: A M
authors.initials: AM
authors.lastname: Svennerholm
mesh_terms: D000907:Antibodies, Bacterial / Q000276:immunology; D000911:Antibodies, Monoclonal / Q000276:immunology; D000942:Antigens, Bacterial / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay; D016480:Helicobacter pylori / Q000254:growth & development / Q000276:immunology*
chemical_list: D000907:Antibodies, Bacterial; D000911:Antibodies, Monoclonal; D000942:Antigens, Bacterial