Pregnancy after immature oocyte donation and intracytoplasmic sperm injection.
OBJECTIVE
To report a case of pregnancy from in vitro-matured primary oocytes fertilized by ICSI. The pregnancy occurred in a woman who was in an oocyte donation program; the woman's husband had normal sperm parameters.


METHODS
Case report.


METHODS
Private general hospital affiliated with a university hospital.


METHODS
A recipient with premature ovarian failure, a recipient's husband with normal sperm, and a pregnant woman who donated her oocytes.


METHODS
Aspiration of immature oocytes during cesarean section, in vitro culture for maturation, ICSI of matured oocytes, coculture of fertilized oocytes.


METHODS
Fertilization of oocytes by ICSI, and cleavage of embryos by Vero cell coculture.


RESULTS
Two of seven immature oocytes became metaphase II oocytes, and both were fertilized by ICSI. The two zygotes were cocultured on Vero cells to become grade 1 two-cell embryos. Pregnancy was obtained after transfer.


CONCLUSIONS
More studies are necessary to clarify whether ICSI can increase the fertilization rate of in vitro-matured primary oocytes, and to clarify the role of coculture in fertilization.
Hwang
J L
JL
Department of Obstetrics and Gynecology, Shin Kong Wu Ho-Su Memorial Hospital, Taipei, Taiwan, Republic of China.
Lin
Y H
YH
Tsai
Y L
YL
D000328:Adult; D002478:Cells, Cultured; D003593:Cytoplasm; D005260:Female; D005307:Fertilization in Vitro / Q000379:methods*; D006801:Humans; D008845:Microinjections; D018587:Oocyte Donation / Q000379:methods*; D009865:Oocytes / Q000254:growth & development*; D011247:Pregnancy; D013396:Suction