Effects of ethanol on rat Sertoli cell function: studies in vitro and in vivo.
Chronic alcohol administration to male animals is associated with testicular atrophy and gonadal failure. The Sertoli cell seems to be the first testicular cell injured as a result of alcohol exposure. To investigate the adverse effects of ethanol on testicular and particularly Sertoli cell function, the consequences of in vivo and in vitro ethanol exposure on rat Sertoli cell mRNA and protein levels of transferrin and ornithine decarboxylase were investigated. In vivo, ethanol exposure enhanced the levels of both hepatic and testicular (Sertoli cell) transferrin protein and mRNA. Ethanol exposure also enhanced testicular, but not hepatic, levels of ornithine decarboxylase protein and mRNA. These in vivo findings were confirmed when isolated Sertoli cells were studied in vitro. Specifically, ethanol exposure increased Sertoli cell transferrin protein and mRNA levels. Ethanol exposure increased Sertoli cell ornithine decarboxylase mRNA and protein when cultured in serum-free media, but not when cultured in the presence of serum. These studies demonstrate that ethanol exposure of rat Sertoli cells is associated with alterations in the levels of mRNA and protein that are known to be important in the process of spermatogenesis. These findings add to the body of evidence that suggests that, within the testes, the Sertoli cell may be an important target for ethanol-induced gonadal injury.
Zhu
Q
Q
Department of Alcoholism and Liver Disease, Oklahoma Medical Research Foundation, Oklahoma City, USA.
Van Thiel
D H
DH
Gavaler
J S
JS
D000437:Alcoholism / Q000473:pathology; D000818:Animals; D000431:Ethanol / Q000633:toxicity*; D015971:Gene Expression Regulation, Enzymologic / Q000187:drug effects; D066298:In Vitro Techniques; D008099:Liver / Q000187:drug effects / Q000473:pathology; D008297:Male; D009955:Ornithine Decarboxylase / Q000187:drug effects / Q000235:genetics; D012333:RNA, Messenger / Q000187:drug effects; D051381:Rats; D017207:Rats, Sprague-Dawley; D012708:Sertoli Cells / Q000187:drug effects* / Q000473:pathology; D013091:Spermatogenesis / Q000187:drug effects*; D014168:Transferrin / Q000187:drug effects / Q000235:genetics
D012333:RNA, Messenger; D014168:Transferrin; D000431:Ethanol; D009955:Ornithine Decarboxylase