Overexpression of phospholipase C-gamma1 in rat 3Y1 fibroblast cells leads to malignant transformation.
Phospholipase C-gamma1 (PLC-gamma1) mediates signals from various extracellular origins to evoke cellular events such as mitogenesis. Previously, we reported that PLC-gamma1 was highly expressed in colorectal cancer and familial adenomatous polyposis, suggesting that PLC-gamma1 might be oncogenic. In this study, we have established rat 3Y1 fibroblasts that overexpress whole PLC-gamma1 and src homology 2 (SH2)-SH2-SH3 domain of PLC-gamma1. These cells showed a transformed phenotype and were tumorigenic when transplanted into nude mice. These results indicate that overexpression of PLC-gamma1 could transform rat fibroblasts, and the transformation is mediated by SH2-SH2-SH3 domain of PLC-gamma1.
Chang
J S
JS
Department of Biology, Daejin University, Pochongun, Kyunggido.
Noh
D Y
DY
Park
I A
IA
Kim
M J
MJ
Song
H
H
Ryu
S H
SH
Suh
P G
PG
D000818:Animals; D002471:Cell Transformation, Neoplastic; D002478:Cells, Cultured; D005347:Fibroblasts / Q000166:cytology* / Q000201:enzymology*; D007527:Isoenzymes / Q000096:biosynthesis / Q000502:physiology*; D051379:Mice; D008819:Mice, Nude; D051966:Phospholipase C gamma; D051381:Rats; D014162:Transfection; D010738:Type C Phospholipases / Q000096:biosynthesis / Q000502:physiology*; D018909:src Homology Domains
D007527:Isoenzymes; D010738:Type C Phospholipases; D051966:Phospholipase C gamma