Supplementary selenium influences the response to fatty acid-induced oxidative stress in humans.
The mutual influences of wheat selenium (Se) and n-3 polyunsaturated fatty acids (n-3 PUFA) on plasma Se and indicators of increased oxidative stress were investigated in a randomized, double-blind study with 31 women (23.5 +/- 3.4 yr). Groups 1 and 2 ingested 5.4 g n-3 PUFA daily (as ethyl esters), whereas groups 3 and 4 received placebo capsules. Groups 2 and 3 received 3 slices of high-Se bread daily, providing 115 micrograms Se, in addition to the 77 +/- 26 micrograms Se in the diet. Groups 1 and 4 received placebo slices. Blood samples were drawn at baseline and at 3 and 6 wk. Serum Se concentrations increased in both groups given Se-enriched bread, but significantly less in subjects given n-3 PUFA (group 2). There were no changes in the plasma ratio alpha-tocopherol:mg cholesterol or plasma ascorbic acid levels. In group 1, plasma-conjugated dienes and thiobarbituric acid-reactive substances (TBARS) rose by 130% (p < 0.005) and 126% (p < 0.005), respectively. Two-way ANOVA showed significant interaction effects of Se and n-3 PUFA on changes in conjugated dienes (p = 0.03) and TBARS (p = 0.015), Se treatment apparently modifying the peroxidative effects of n-3 PUFA. In subjects receiving n-3 PUFA, changes in conjugated dienes and TBARS were negatively correlated with changes in serum Se. In summary, n-3 PUFA modified the effect of Se supplementation, whereas Se seemed to modify the peroxidative effects of n-3 PUFA.
Meltzer
H M
HM
Nordic School of Nutrition, Faculty of Medincine, University of Oslo, Norway.
Folmer
M
M
Wang
S
S
Lie
O
O
Maage
A
A
Mundal
H H
HH
Ydersbond
T A
TA
D001205:Ascorbic Acid / Q000097:blood; D002784:Cholesterol / Q000097:blood; D004311:Double-Blind Method; D005231:Fatty Acids, Unsaturated / Q000008:administration & dosage* / Q000097:blood; D005260:Female; D006801:Humans; D015227:Lipid Peroxidation; D009664:Norway; D018384:Oxidative Stress / Q000187:drug effects*; D012643:Selenium / Q000008:administration & dosage* / Q000097:blood; D013054:Spectrophotometry, Atomic; D017392:Thiobarbituric Acid Reactive Substances / Q000032:analysis; D014810:Vitamin E / Q000097:blood
D005231:Fatty Acids, Unsaturated; D017392:Thiobarbituric Acid Reactive Substances; D014810:Vitamin E; D002784:Cholesterol; D012643:Selenium; D001205:Ascorbic Acid