[Replacement of the anterior cruciate ligament by cold preserved bone-cruciate ligament-bone allotransplants. An experimental study in the sheep].
One ACL in each of 17 mature sheep was replaced with a deep-frozen bone-an ACL bone allograft. Allografts were obtained from skeletally mature sheep using a standard aseptic technique and stored deep frozen for at least 6 days (mean 21 days). Macroscopical, biomechanical, and histological changes were evaluated 12, 24, and 52 weeks following implantation. At autopsy all allograft ligaments were present and demonstrated no evidence of infection or immune reaction. We found slight arthrotic changes in 3 knees after 12 weeks, in 4 knees after 24 weeks, and in 3 knees after 52 weeks. Twelve weeks after the operation the maximum load of the allografts was 17.5% of the contralateral controls and increased to 20.9% after 24 weeks and to 32% of controls after 52 weeks. Ligament stiffness in the linear region also increased from 18.9% of control (12 weeks) to 32.5% after 52 weeks, whereas maximum load decreased from 112.2% of controls (12 weeks) to 98% of controls (52 weeks). Histologically, the allografts progressively matured with time, becoming nearly identical to normal ligaments at 52 weeks.
Jaskulka
R
R
Unfallchirurgische Abteilung, Sozialmedizinisches Zentrum Ost, Wien.
Ittner
G
G
Birkner
T
T
D000818:Animals; D016118:Anterior Cruciate Ligament / Q000473:pathology / Q000637:transplantation*; D001696:Biomechanical Phenomena; D016025:Bone Transplantation / Q000473:pathology*; D005260:Female; D006084:Graft Rejection / Q000473:pathology; D016059:Range of Motion, Articular / Q000502:physiology; D012756:Sheep; D014184:Transplantation, Homologous; D016474:Weight-Bearing / Q000502:physiology; D014945:Wound Healing / Q000502:physiology