Mismatch-sensitive hybridization detection by peptide nucleic acids immobilized on a quartz crystal microbalance.
A quartz crystal microbalance DNA hybridization biosensor, based on thiol-derivatized peptide nucleic acid (PNA) probes, offers unusual in situ differentiation of single-base mismatches. A large excess of a single-base mismatch oligonucleotide has no effect on the frequency response of the target. Such remarkable distinction between perfect matches and mismatches is illustrated by the detection of a common mutation in the p53 gene. The greater specificity of the new mass-sensitive indicatorless hybridization device over those of analogous PNA-based carbon electrodes is attributed to the formation of a PNA monolayer and the use of a hydrophilic ethylene glycol linker. The improved specificity is coupled to very fast (3-5 min) hybridization in a low-ionic-strength medium.
Wang
J
J
Department of Chemistry and Biochemistry, New Mexico State University, Las Cruces 88003, USA.
Nielsen
P E
PE
Jiang
M
M
Cai
X
X
Fernandes
J R
JR
Grant
D H
DH
Ozsoz
M
M
Beglieter
A
A
Mowat
M
M
D015374:Biosensing Techniques; D016158:Genes, p53; D009692:Nucleic Acid Heteroduplexes; D009693:Nucleic Acid Hybridization; D015345:Oligonucleotide Probes; D010455:Peptides
D009692:Nucleic Acid Heteroduplexes; D015345:Oligonucleotide Probes; D010455:Peptides