title: Toxin I, but not 4-aminopyridine, blocks the late inhibitory component of the dorsal root reflex in an isolated preparation of rat spinal cord.
abstract: Bursts of spontaneous antidromic dorsal root action potentials, and evoked dorsal root reflexes (DRR), have been recorded from lumbar roots of isolated spinal cord preparations of rats weighing 70-90 g. The pattern of dorsal root activity was similar to that reported for isolated cord preparations from hamsters, but the frequency of spontaneous dorsal root activity was approximately 10 times slower in the rat. Toxin I and 4-aminopyridine (4-AP) both increased the frequency of spontaneous dorsal root activity. The threshold concentration of 4-AP was 1 microM, with EC50 at 20 microM. Insufficient Toxin I was available to reach a maximal response, but the threshold concentration for producing an increase in spontaneous activity was 0.1 microM, and the curve appeared to be parallel to that of 4-AP. The patterns of spontaneous dorsal root activity in the presence of 4-AP and Toxin I differed. In 4-AP bursts of large amplitude action potentials were followed by periods of depressed activity lasting up to 500 ms, whereas in Toxin I bursts of large amplitude action potentials caused no change in the continuously firing small amplitude action potentials. DRR evoked by stimulation of adjacent dorsal roots also showed differences in the presence of 4-AP and Toxin I. In 4-AP the excitatory phase of the reflex was followed by a period of depressed activity lasting up to 500 ms. This was was reduced or absent in the presence of Toxin I. Paired pulse stimulation confirmed the presence of inhibition in 4-AP, and its reduction in Toxin I. Examination of the pattern of spontaneous dorsal root activity following dorsal root stimulation showed strong oscillatory activity in the presence of 4-AP, but little such activity in the presence of Toxin I. It was concluded that the actions of 4-AP and Toxin I on the isolated preparation of rat spinal cord are similar in that both cause an increase in the spontaneous dorsal root firing rate, but that Toxin I also blocks the period of inhibition which follows bursts of large amplitude action potentials.
authors.affiliation: Department of Physiology and Pharmacology, University of Southampton, UK. jb@soton.ac.uk
authors.forename: J
authors.initials: J
authors.lastname: Bagust
authors.forename: L
authors.initials: L
authors.lastname: Zhang
authors.forename: D
authors.initials: D
authors.lastname: Owen
mesh_terms: D015761:4-Aminopyridine / Q000494:pharmacology*; D000200:Action Potentials / Q000187:drug effects* / Q000502:physiology; D000818:Animals; D006224:Cricetinae; D004546:Elapid Venoms / Q000494:pharmacology*; D004558:Electric Stimulation; D066298:In Vitro Techniques; D051381:Rats; D017208:Rats, Wistar; D012018:Reflex / Q000187:drug effects* / Q000502:physiology; D013116:Spinal Cord / Q000187:drug effects / Q000502:physiology*; D013126:Spinal Nerve Roots / Q000187:drug effects / Q000502:physiology*
chemical_list: D004546:Elapid Venoms; C108582:dendrotoxin I; D015761:4-Aminopyridine