Allelotype analysis of adenocarcinoma of the gastric cardia.
To identify chromosomal loci involved in the development of proximal gastric adenocarcinoma, this study delineated the pattern of allelic imbalance in a series of 38 adenocarcinomas arising in the gastric cardia. A total of 137 microsatellite markers covering all autosomal arms, excluding acrocentric arms, were analysed. A mean of 35 out of a total of 39 chromosomal arms studied were informative for each patient. The tumour group demonstrated a high level of allelic imbalance, with an observed median fractional allelic imbalance of 0.47 for the 29 intestinal-type adenocarcinomas and 0.54 for the nine diffuse-type adenocarcinomas. Allelic imbalance was detected in >50% of informative cases in both histological subtypes on a number of chromosomal arms. In the intestinal subtype, these included, 3p (61%), 4q (71%), 5q (59%), 8p (60%), 9p (65%), 9q (83%), 12q (52%), 13q (52%), 17p (78%) and 18q (70%). A higher incidence of allelic imbalance was detected on chromosome 16q in tumours of the diffuse type relative to those of the intestinal type. A more detailed mapping on chromosomes 4q and 6q identified a number of cases with subchromosomal breakpoints. In conclusion, this analysis has indicated regions of the genome potentially involved in the development of proximal gastric carcinomas.
Gleeson
C M
CM
Department of Medical Genetics, The Queen's University of Belfast, Belfast City Hospital, UK.
Sloan
J M
JM
McGuigan
J A
JA
Ritchie
A J
AJ
Weber
J L
JL
Russell
S E
SE
D000230:Adenocarcinoma / Q000235:genetics* / Q000473:pathology; D000483:Alleles; D002299:Cardia; D002869:Chromosome Aberrations; D019457:Chromosome Breakage; D002874:Chromosome Mapping; D002894:Chromosomes, Human, Pair 4; D002896:Chromosomes, Human, Pair 6; D004273:DNA, Neoplasm / Q000235:genetics; D005819:Genetic Markers; D006801:Humans; D018895:Microsatellite Repeats; D011110:Polymorphism, Genetic; D013274:Stomach Neoplasms / Q000235:genetics* / Q000473:pathology
D004273:DNA, Neoplasm; D005819:Genetic Markers