Quantitative measurements of released amines from individual exocytosis events.
Chemical analysis of single cells is an area of great interest in the biological sciences. Single-cell systems are being utilized as a model to understand in vivo processes better. One method that is moving to the forefront in cellular analysis is electrochemistry. Owing to their rapid response time and small dimensions, voltammetric microelectrode techniques, such as amperometry and fast-scan voltammetry, have made it possible to monitor minute amounts of biological compounds and transiently occurring chemical events in cellular systems. The application of these methods to the quantitation of individual vesicular release events from single cells is overviewed here. The application of electrochemical monitoring to several types of cultured cells, including bovine adrenal chromaffin cells, rat pheochromocytoma (PC12) cells, beige mouse mast cells, superior cervical ganglion neurons, and human pancreatic beta-cells, as well as to the invertebrate systems, the leech Hirudo medicinalis, and pond snail Planorbis corneus has provided a wealth of new information concerning exocytosis. Results obtained from the studies highlight the potential of electrochemical techniques in cellular analysis to contribute to our understanding of molecular and pharmacological effects on exocytosis. This article overviews work done on all the above cell types with an emphasis on PC12 cells.
Clark
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Department of Chemistry, Pennsylvania State University, University Park 16802, USA.
Ewing
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D000310:Adrenal Gland Neoplasms; D000313:Adrenal Medulla / Q000502:physiology; D000818:Animals; D002395:Catecholamines / Q000032:analysis / Q000378:metabolism*; D002417:Cattle; D002478:Cells, Cultured; D019439:Chromaffin Cells / Q000502:physiology; D004563:Electrochemistry / Q000379:methods; D005089:Exocytosis; D006801:Humans; D007515:Islets of Langerhans / Q000502:physiology; D008407:Mast Cells / Q000502:physiology; D051379:Mice; D008817:Mice, Mutant Strains; D009474:Neurons / Q000502:physiology; D016716:PC12 Cells; D010673:Pheochromocytoma; D051381:Rats; D017783:Superior Cervical Ganglion / Q000502:physiology
D002395:Catecholamines