Stable isotope ratio analysis of amino acids: the use of N(O,S)-ethoxycarbonyl trifluoroethyl ester derivatives and gas chromatography/mass spectrometry.
N(O,S)-Ethoxycarbonyl trifluoroethyl amino acid esters are formed by the reaction of amino acids with ethylchloroformate plus trifluoroethanol plus pyridine. The use of these derivatives for a rapid and sensitive determination of specific enrichment of stable isotopically labeled tracer amino acids in blood plasma and protein hydrolysates, by using gas chromatography/electron impact mass spectrometry, is discussed.
Pietzsch
J
J
Institute of Clinical Metabolic Research, Medical Faculty Carl Gustav Carus, Technical University Dresden, Germany.
Julius
U
U
Hanefeld
M
M
D000596:Amino Acids / Q000032:analysis* / Q000737:chemistry; D001053:Apolipoproteins / Q000737:chemistry / Q000378:metabolism; D002138:Calibration; D002247:Carbon Isotopes; D003903:Deuterium; D005563:Formic Acid Esters; D008401:Gas Chromatography-Mass Spectrometry; D006801:Humans; D007700:Kinetics; D008954:Models, Biological; D014270:Trifluoroethanol
D000596:Amino Acids; D001053:Apolipoproteins; D002247:Carbon Isotopes; D005563:Formic Acid Esters; C007658:ethyl chloroformate; D014270:Trifluoroethanol; D003903:Deuterium