Modulation of fructose-2,6-bisphosphate metabolism by components of the extracellular matrix in cultured cells. Interaction with epidermal growth factor.
The use of NIH3T3 fibroblasts overexpressing different mutations of the EGF receptor shows that regulation of fructose-2,6-bisphosphate (Fru-2,6-P2) metabolism by EGF is mediated by the kinase activity of the EGF receptor and suggests a PLCgamma1-mediated mechanism. The effect of several extracellular matrix components on glucose metabolism was assessed by incubating A431 cells and NIH3T3 fibroblasts with heparin, laminin, fibronectin, collagen and PG-I and PG-II proteoglycans and measuring the levels of Fru-2,6-P2. Laminin increased the levels of Fru-2,6-P2 and heparin decreased the levels of the metabolite, whereas the other molecules did not have any effect. No effect of laminin or heparin in glucose uptake by the cell was observed. Laminin was able to modulate the effects of EGF on Fru-2,6-P2 concentration, suggesting cross-talk between these agents.
Baulida
J
J
Departament de Bioquímica i Biologia Molecular, Facultat de Veterinària, Universitat Autònoma de Barcelona, Bellaterra, Spain.
Onetti
R
R
Bassols
A
A
D016475:3T3 Cells; D000818:Animals; D002460:Cell Line; D003094:Collagen / Q000494:pharmacology; D004815:Epidermal Growth Factor / Q000494:pharmacology*; D066246:ErbB Receptors / Q000378:metabolism; D016326:Extracellular Matrix Proteins / Q000494:pharmacology*; D005353:Fibronectins / Q000494:pharmacology; D005635:Fructosediphosphates / Q000378:metabolism*; D006493:Heparin / Q000494:pharmacology; D006801:Humans; D007527:Isoenzymes / Q000378:metabolism; D007700:Kinetics; D007797:Laminin / Q000494:pharmacology; D051379:Mice; D051966:Phospholipase C gamma; D011509:Proteoglycans / Q000494:pharmacology; D010738:Type C Phospholipases / Q000378:metabolism
D016326:Extracellular Matrix Proteins; D005353:Fibronectins; D005635:Fructosediphosphates; D007527:Isoenzymes; D007797:Laminin; D011509:Proteoglycans; D004815:Epidermal Growth Factor; C027652:fructose 2,6-diphosphate; D006493:Heparin; D003094:Collagen; D066246:ErbB Receptors; D010738:Type C Phospholipases; D051966:Phospholipase C gamma