title: Involvement of afsA in A-factor biosynthesis as a key enzyme.
abstract: The afsA gene of Streptomyces griseus has been postulated to encode a key enzyme for A-factor biosynthesis from primary metabolites commonly present in Streptomyces strains. Escherichia coli cells harboring afsA under the control of the T7 promoter specified distinct A-factor activity in the culture broth, as determined by induction of streptomycin production and aerial mycelium and spore formation in an A-factor-deficient S. griseus mutant strain. Production of the substance(s) having A-factor activity was inhibited by cerulenin, an inhibitor of fatty acid biosynthesis. These observations suggest that afsA encodes a key enzyme in the A-factor biosynthetic pathway in which a beta-keto acid derived from fatty acid biosynthesis and a glycerol derivative serve as precursors.
authors.affiliation: Department of Biotechnology, Graduate School of Agriculture and Life Sciences, University of Tokyo, Japan.
authors.forename: N
authors.initials: N
authors.lastname: Ando
authors.forename: N
authors.initials: N
authors.lastname: Matsumori
authors.forename: S
authors.initials: S
authors.lastname: Sakuda
authors.forename: T
authors.initials: T
authors.lastname: Beppu
authors.forename: S
authors.initials: S
authors.lastname: Horinouchi
mesh_terms: D015107:4-Butyrolactone / Q000031:analogs & derivatives* / Q000096:biosynthesis; D001426:Bacterial Proteins / Q000235:genetics* / Q000378:metabolism*; D002569:Cerulenin / Q000494:pharmacology; D002851:Chromatography, High Pressure Liquid; D004926:Escherichia coli / Q000187:drug effects / Q000235:genetics / Q000378:metabolism; D005227:Fatty Acids / Q000096:biosynthesis; D006133:Growth Substances / Q000096:biosynthesis*; D008954:Models, Biological; D009154:Mutation; D011994:Recombinant Proteins / Q000378:metabolism; D013305:Streptomyces griseus / Q000235:genetics / Q000378:metabolism; D013307:Streptomycin / Q000096:biosynthesis
chemical_list: C108224:AfsA protein, Streptomyces griseus; D001426:Bacterial Proteins; D005227:Fatty Acids; D006133:Growth Substances; D011994:Recombinant Proteins; D002569:Cerulenin; C025035:A-factor (Streptomyces); D015107:4-Butyrolactone; D013307:Streptomycin