Evidence that human Fc gamma receptor IIA (CD32) subtypes are not receptors for oxidized LDL.
Several lines of evidence suggest that clearance of oxidized LDL (oxLDL) immune complexes by macrophage IgG Fc receptors (Fc gamma Rs) plays a role in atherogenesis. Ox-LDL may also be cleared directly by Fc gamma Rs, as shown for murine Fc gamma RII-B2. In humans, the homologous Fc gamma R is Fc gamma RIIA (CD32), which is abundantly expressed on monocytes and macrophages and shares 60% sequence identity with murine Fc gamma RII-B2. As murine Fc gamma RII-B2 and human Fc gamma RIIA also share similar IgG ligand-binding properties, the purpose of this study was to test the hypothesis that human CD32 is a receptor for oxLDL. For these studies we used transfected Chinese hamster ovary (CHO) cells, monocytes, and cell lines that functionally express either of two Fc gamma RIIA subtypes (R131 or H131) and assayed binding or degradation of several preparations of oxLDL. The integrity of all oxLDL preparations was checked by studying their ability to react with CHO cells expressing human type I scavenger receptors and by other characteristics of lipoprotein oxidation. Although we showed that each preparation of oxLDL could recognize class A or class B scavenger receptors, we did not detect any differences in the binding or degradation of any type of oxLDL preparation among control versus CHO cell transfectants. Using monocytes that express Fc gamma RIIA and CD36, we showed that the binding of oxLDL was inhibited by antibodies to CD36, but not by Fc gamma RIIA antibodies. Thus, the data do not support the hypothesis that human Fc gamma RIIA is by itself a receptor for oxLDL. We conclude that human CD32 can mediate uptake of lipoprotein immune complexes, but does not mediate uptake of oxLDL in the absence of anti-oxLDL antibodies. OxLDL may interact with human mononuclear phagocytes directly via other types of receptors, such as class A and class B scavenger receptors or CD68.
Morganelli
P M
PM
Veterans Administration Hospital, White River Junction, VT 05009, USA. Peter.Morganelli@Dartmouth.edu
Groveman
D S
DS
Pfeiffer
J R
JR
D000818:Animals; D000936:Antigen-Antibody Complex / Q000378:metabolism; D018955:CD36 Antigens; D016466:CHO Cells; D002478:Cells, Cultured; D003300:Copper / Q000494:pharmacology; D006224:Cricetinae; D003412:Cricetulus; D006801:Humans; D015227:Lipid Peroxidation; D008077:Lipoproteins, LDL / Q000276:immunology / Q000378:metabolism*; D008565:Membrane Proteins; D009000:Monocytes / Q000378:metabolism; D016877:Oxidants / Q000494:pharmacology; D010084:Oxidation-Reduction; D011485:Protein Binding; D017452:Receptors, IgG / Q000145:classification / Q000378:metabolism*; D011971:Receptors, Immunologic / Q000378:metabolism; D018110:Receptors, Lipoprotein; D051116:Receptors, Scavenger; D011993:Recombinant Fusion Proteins / Q000378:metabolism; D051117:Scavenger Receptors, Class A; D051122:Scavenger Receptors, Class B; D014162:Transfection; D014407:Tumor Cells, Cultured
D000936:Antigen-Antibody Complex; D018955:CD36 Antigens; D008077:Lipoproteins, LDL; D008565:Membrane Proteins; D016877:Oxidants; D017452:Receptors, IgG; D011971:Receptors, Immunologic; D018110:Receptors, Lipoprotein; D051116:Receptors, Scavenger; D011993:Recombinant Fusion Proteins; C495124:SCARB1 protein, human; C454739:Scarb1 protein, mouse; D051117:Scavenger Receptors, Class A; D051122:Scavenger Receptors, Class B; C099252:oxidized low density lipoprotein; D003300:Copper