Levels of biogenic amines in larvae and adults of the rat hookworm, Nippostrongylus brasiliensis (Nematoda).
Norepinephrine, 5-hydroxytryptophan, octopamine, dihydroxyphenylacetic acid, N-acetyldopamine, dopamine, 5-hydroxyindoleacetic acid, N-acetylserotonin, tyramine, tryptophan and serotonin in larvae (third free stage and parasitic stages) and adult males and females (at defined ages during the intestinal phase) of the parasitic nematode Nippostrongylus brasiliensis were quantified simultaneously by high-performance liquid chromatography with electrochemical detection. Biogenic amine levels depended on the stage, the age and the sex of parasites and on environmental conditions. Their physiological roles in reproductively competent adults of this nematode are discussed in relation to exuviation and egg laying. Parallel fluctuations in free ecdysteroids and norepinephrine were observed in females from the same worm populations.
Goudey-Perrière
F
F
Laboratoire de Biologie et Contrôle des Organismes Parasites, Faculté de Pharmacie, Université de Paris-Sud, Châtenay-Malabry, France.
Grosclaude
J M
JM
Nembo
B
B
Barreteau
H
H
Jacquot
C
C
Gayral
P
P
D015102:3,4-Dihydroxyphenylacetic Acid / Q000378:metabolism; D006916:5-Hydroxytryptophan / Q000378:metabolism; D000818:Animals; D015306:Biogenic Monoamines / Q000378:metabolism*; D004298:Dopamine / Q000031:analogs & derivatives / Q000378:metabolism; D026461:Ecdysteroids; D005260:Female; D006897:Hydroxyindoleacetic Acid / Q000378:metabolism; D007301:Insect Hormones / Q000378:metabolism; D007814:Larva / Q000378:metabolism; D008297:Male; D009559:Nippostrongylus / Q000254:growth & development / Q000378:metabolism* / Q000472:pathogenicity; D009638:Norepinephrine / Q000378:metabolism; D009655:Octopamine / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D012701:Serotonin / Q000031:analogs & derivatives / Q000378:metabolism; D012727:Sex Characteristics; D013256:Steroids / Q000378:metabolism; D014364:Tryptophan / Q000378:metabolism; D014439:Tyramine / Q000378:metabolism
D015306:Biogenic Monoamines; D026461:Ecdysteroids; D007301:Insect Hormones; D013256:Steroids; D015102:3,4-Dihydroxyphenylacetic Acid; D009655:Octopamine; C006374:N-acetyldopamine; D012701:Serotonin; D006897:Hydroxyindoleacetic Acid; D014364:Tryptophan; D006916:5-Hydroxytryptophan; C006389:N-acetylserotonin; D004298:Dopamine; D009638:Norepinephrine; D014439:Tyramine