title: New core promoter element in RNA polymerase II-dependent transcription: sequence-specific DNA binding by transcription factor IIB.
abstract: A sequence element located immediately upstream of the TATA element, and having the consensus sequence 5'-G/C-G/C-G/A-C-G-C-C-3', affects the ability of transcription factor IIB to enter transcription complexes and support transcription initiation. The sequence element is recognized directly by the transcription factor IIB. Recognition involves alpha-helices 4' and 5' of IIB, which comprise a helix-turn-helix DNA-binding motif. These observations establish that transcription initiation involves a fourth core promoter element, the IIB recognition element (BRE), in addition to the TATA element, the initiator element, and the downstream promoter element, and involves a second sequence-specific general transcription factor, IIB, in addition to transcription factor IID.
authors.affiliation: Howard Hughes Medical Institute and Department of Biochemistry, University of Medicine and Dentistry of New Jersey-Robert Wood Johnson Medical School, Piscataway, New Jersey 08854, USA.
authors.forename: T
authors.initials: T
authors.lastname: Lagrange
authors.forename: A N
authors.initials: AN
authors.lastname: Kapanidis
authors.forename: H
authors.initials: H
authors.lastname: Tang
authors.forename: D
authors.initials: D
authors.lastname: Reinberg
authors.forename: R H
authors.initials: RH
authors.lastname: Ebright
mesh_terms: D000595:Amino Acid Sequence; D004268:DNA-Binding Proteins / Q000378:metabolism*; D019077:Helix-Turn-Helix Motifs; D006801:Humans; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation; D011401:Promoter Regions, Genetic; D012319:RNA Polymerase II / Q000378:metabolism* / Q000494:pharmacology; D035581:Transcription Factor TFIIB; D014157:Transcription Factors / Q000378:metabolism*; D014158:Transcription, Genetic
chemical_list: D004268:DNA-Binding Proteins; D035581:Transcription Factor TFIIB; D014157:Transcription Factors; D012319:RNA Polymerase II