Implantation on the suture material and efficacy of povidone-iodine solution.
Suture implantation of viable exfoliated tumour cells may be responsible for local recurrence of colorectal cancer. Using a colon cancer cell line, we obtained a suture implantation without intraperitoneal metastasis in about 80% of the control animals, when sacrificed on the 2nd postoperative week. The cytotoxic efficacy of povidone-iodine (PVP-I) was tested in vivo by a rat model with viable intracaecal tumour cells, and in vitro by trypan blue exclusion and the MTT assay. In vivo PVP-I at 5% significantly reduced the incidence of tumour growth, while the product at 2.5% had a significant effect in only the monofilament polypropylene group. In an in vitro toxicity study, PVP-I higher than 0.16% was effective at killing almost all tumour cells. PVP-I had effective cytotoxicity in vivo and in vitro, being less cytotoxic in vivo than in vitro.
Tsunoda
A
A
Second Department of Surgery, School of Medicine, Showa University, Tokyo, Japan.
Shibusawa
M
M
Tsunoda
Y
Y
Choh
H
H
Takata
M
M
Kusano
M
M
D000818:Animals; D016923:Cell Death / Q000187:drug effects; D003110:Colonic Neoplasms / Q000473:pathology / Q000601:surgery; D015179:Colorectal Neoplasms / Q000473:pathology / Q000601:surgery; D066298:In Vitro Techniques; D008297:Male; D009364:Neoplasm Recurrence, Local / Q000517:prevention & control; D009366:Neoplasm Seeding; D011206:Povidone-Iodine / Q000008:administration & dosage*; D051381:Rats; D011916:Rats, Inbred F344; D012996:Solutions; D013537:Sutures / Q000009:adverse effects*; D014407:Tumor Cells, Cultured
D012996:Solutions; D011206:Povidone-Iodine