The rat S-adenosylhomocysteine hydrolase promoter.
The 1.2-kb DNA sequence flanking the transcription start of the AdoHcy hydrolase gene was cloned into the luciferase reporter plasmid pGL3-basic, and promoter activity was measured in transiently transfected CHO cells. Deletion analysis showed that most promoter activity was located within a 153 bp fragment immediately upstream from the predominant transcription start. The 153 bp fragment includes sites for AP-2, glucocorticoid-responsive element, SP-1, and a TATA-like sequence TATTTAAA. Mutational analysis demonstrated that the SP-1 site nearest the start of transcription contributed significantly to promoter activity, whereas, the other elements, including the appropriately positioned TATTTAAA sequence, had little affect on promoter activity.
Merta
A
A
Laboratory of General and Comparative Biochemistry, National Institute of Mental Health, Bethesda, Maryland 20892, USA.
Aksamit
R R
RR
Cantoni
G L
GL
D043383:Adenosylhomocysteinase; D000818:Animals; D001483:Base Sequence; D015152:Blotting, Northern; D016466:CHO Cells; D003001:Cloning, Molecular; D006224:Cricetinae; D004252:DNA Mutational Analysis; D017931:DNA Primers; D017930:Genes, Reporter; D006867:Hydrolases / Q000235:genetics* / Q000378:metabolism; D008969:Molecular Sequence Data; D016296:Mutagenesis / Q000235:genetics; D011401:Promoter Regions, Genetic / Q000235:genetics*; D012333:RNA, Messenger / Q000032:analysis / Q000235:genetics; D051381:Rats; D017384:Sequence Deletion; D014162:Transfection / Q000235:genetics
D017931:DNA Primers; D012333:RNA, Messenger; D006867:Hydrolases; D043383:Adenosylhomocysteinase