Nucleotide sequence comparison of the IgE constant region in patients with atopic dermatitis and non-atopic individuals.
In order to answer the question whether the Fc portion of the IgE molecule in patients with atopic dermatitis is altered by somatic replacement mutations, we amplified and sequenced the respective C epsilon 2 and C epsilon 3 domain genes. Five patients with atopic dermatitis and 6 non-atopic individuals were studied. Neither within the C epsilon 2 nor the C epsilon 3 domain could any common nucleotide substitutions be detected. Therefore, the conclusion can be drawn that, in patients with atopic dermatitis, there are no protein sequence differences within the Fc part of the IgE which could be responsible for distinct functional features with regard to Fc epsilon-receptor binding and signal transduction or could account for the frequent occurrence of anti-IgE autoantibodies in these individuals.
Rutz
S
S
Department of Dermatology, University Hospital (Charité), Humboldt-University Berlin, Germany.
Siegel
P
P
Muche
M
M
Sterry
W
W
Jahn
S
S
D001483:Base Sequence; D004247:DNA; D003876:Dermatitis, Atopic / Q000235:genetics* / Q000276:immunology; D006801:Humans; D007127:Immunoglobulin Constant Regions / Q000235:genetics*; D007073:Immunoglobulin E / Q000235:genetics*; D007141:Immunoglobulin Fc Fragments / Q000235:genetics; D008969:Molecular Sequence Data
D007127:Immunoglobulin Constant Regions; D007141:Immunoglobulin Fc Fragments; D007073:Immunoglobulin E; D004247:DNA