Molecular reconstruction of Sleeping Beauty, a Tc1-like transposon from fish, and its transposition in human cells.
Members of the Tc1/mariner superfamily of transposons isolated from fish appear to be transpositionally inactive due to the accumulation of mutations. Molecular phylogenetic data were used to construct a synthetic transposon, Sleeping Beauty, which could be identical or equivalent to an ancient element that dispersed in fish genomes in part by horizontal transmission between species. A consensus sequence of a transposase gene of the salmonid subfamily of elements was engineered by eliminating the inactivating mutations. Sleeping Beauty transposase binds to the inverted repeats of salmonid transposons in a substrate-specific manner, and it mediates precise cut-and-paste transposition in fish as well as in mouse and human cells. Sleeping Beauty is an active DNA-transposon system from vertebrates for genetic transformation and insertional mutagenesis.
Ivics
Z
Z
Department of Genetics and Cell Biology, University of Minnesota, St. Paul 55108-1095, USA.
Hackett
P B
PB
Plasterk
R H
RH
Izsvák
Z
Z
D000595:Amino Acid Sequence; D000818:Animals; D002347:Carps; D002460:Cell Line; D004247:DNA / Q000378:metabolism; D004251:DNA Transposable Elements / Q000235:genetics*; D004274:DNA, Recombinant / Q000235:genetics; D004789:Enzyme Activation; D006801:Humans; D051379:Mice; D008969:Molecular Sequence Data; D011993:Recombinant Fusion Proteins; D012487:Salmonidae / Q000235:genetics*; D014162:Transfection; D019895:Transposases / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism*
D004251:DNA Transposable Elements; D004274:DNA, Recombinant; D011993:Recombinant Fusion Proteins; D004247:DNA; D019895:Transposases