CGRP-beta unlike CGRP-alpha has no osteogenic stimulatory effect in vitro.
The purpose of this study is to test whether CGRP-beta has an osteogenic stimulating effect, as does CGRP-alpha, on rat bone marrow cells in vitro. CGRP-beta in different doses was added daily to bone marrow white cells, which were harvested from rats, then seeded onto a previously prepared layer of fibroblasts. CGRP-alpha in different doses was used as a positive control. Fourteen days after the start of the experiment, there was no statistical difference in the number of bone colonies between the control and CGRP-beta dishes. The CGRP-alpha dishes demonstrated an increase in the number of colonies with an increase of peptide dose.
Hirt
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Department of Neurobiology, School of Medicine and Oral Biology, School of Dentistry UCLA 90095, USA.
Bernard
G W
GW
D000595:Amino Acid Sequence; D000818:Animals; D015740:Calcitonin Gene-Related Peptide / Q000494:pharmacology*; D002478:Cells, Cultured; D008297:Male; D008969:Molecular Sequence Data; D010012:Osteogenesis / Q000187:drug effects*; D051381:Rats; D017207:Rats, Sprague-Dawley; D013268:Stimulation, Chemical
D015740:Calcitonin Gene-Related Peptide