Role of lipoprotein-X in foam cell formation and rat mesangial cell proliferation.
1. In the present study, the effect of reconstituted lipoprotein-X (rLp-X) on lipid accumulation and foam cell formation in rat peritoneal macrophages was evaluated. Furthermore, the combined effect of rLp-X and macrophages on mesangial cell proliferation was examined. 2. Incubation of macrophages with rLp-X (177 and 387 nmol unesterified cholesterol (FC)/mL) resulted in an increase of cellular cholesterol (162%) and cholesteryl esters (223 to 245%) relative to control. 3. Oil Red O staining of macrophages treated with rLp-X revealed the presence of foam cells. 4. In conclusion, rLp-X had no effect on the proliferation of mesangial cells incubated in macrophage-conditioned medium.
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Department of Pathology and Laboratory Medicine, University of British Columbia, Vancouver, Canada.
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D000818:Animals; D002450:Cell Communication / Q000502:physiology; D002455:Cell Division / Q000502:physiology; D002478:Cells, Cultured; D005487:Foam Cells / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism; D005920:Glomerular Mesangium / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism; D008055:Lipids / Q000493:pharmacokinetics; D008073:Lipoprotein-X / Q000378:metabolism / Q000494:pharmacology / Q000502:physiology*; D017737:Macrophages, Peritoneal / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley
D008055:Lipids; D008073:Lipoprotein-X