title: Inhibition of gill Na+,K+-ATPase activity in the eel, Anguilla anguilla, by fenitrothion.
abstract: European eels (Anguilla anguilla) were exposed to sublethal fenitrothion concentrations (0.02 and 0.04 mg/liter) in a continuous flow-through system for 4 days. Gill Mg2+- and Na+,K+-ATPase activities were evaluated after 2, 8, 12, 24, 32, 48, 56, 72, and 96 h of pesticide exposure. Results indicated that ATPase activity in gill tissue decreased as concentration of fenitrothion increased. Pesticide induced significant inhibitory effects on the Na+, K+-ATPase activity of A. anguilla, ranging from >56% inhibition at a sublethal concentration of 0.02 ppm to >73% inhibition at a sublethal concentration of 0.04 ppm. Eels were exposed to both fenitrothion concentrations for 96 h and then allowed a period of recovery in pesticide-free water. Samples were removed at 8, 12, 24, 48, 72, 96, 144, and 192 h and eel gill ATPase activity was evaluated. Following 1 week of recovery, the Na+,K+-ATPase activity for those animals previously exposed to 0.04 ppm fenitrothion was still different from that of the controls.
authors.affiliation: Department of Animal Physiology, Faculty of Biological Sciences, Dr. Moliner 50, Burjasot, Valencia, 46100, Spain.
authors.forename: E
authors.initials: E
authors.lastname: Sancho
authors.forename: M D
authors.initials: MD
authors.lastname: Ferrando
authors.forename: E
authors.initials: E
authors.lastname: Andreu
mesh_terms: D000811:Anguilla / Q000502:physiology*; D000818:Animals; D004305:Dose-Response Relationship, Drug; D004785:Environmental Pollutants; D005278:Fenitrothion / Q000633:toxicity*; D005880:Gills / Q000201:enzymology; D007306:Insecticides / Q000633:toxicity*; D000254:Sodium-Potassium-Exchanging ATPase / Q000187:drug effects* / Q000378:metabolism*
chemical_list: D004785:Environmental Pollutants; D007306:Insecticides; D000254:Sodium-Potassium-Exchanging ATPase; D005278:Fenitrothion