Characterization of a recombinant Onchocerca volvulus antigen (Ov33) produced in yeast.
A yeast (Saccharomyces cerevisiae) expression system has been adapted to produce reagent quantities of a major Onchocerca antigen, Ov33. Using a pool of monoclonal antibodies produced against third-stage larvae, a cDNA library constructed from adult O. volvulus worms was screened. Twenty-seven cDNAs were isolated, two of which had sequence homology to Ov33, a putative aspartyl protease inhibitor, which is the immunodominant antigen of O. volvulus. These cDNAs were expressed at high levels intracellularly or through the secretory pathway of S. cerevisiae. Localization studies using antisera produced against purified recombinant protein demonstrated that Ov33 is a very abundant parasite protein present in the hypodermis, muscle, and uterus of female worms, as well as in embryonic microfilariae. The soluble recombinant protein secreted by yeast (C71) demonstrated inhibitory activity against the aspartyl protease pepsin. Antibodies to the recombinant protein-mediated leukocyte adherence to and killing of skin microfilariae. The sensitivity of a diagnostic test using recombinant Ov33 was evaluated using sera from 441 patients. The mean sensitivities for the two recombinant constructs, C27 and C71, were 82.2% and 85.4%, respectively. The combined sensitivity using both recombinant proteins was 94%.
Tume
C B
CB
Faculty of Medicine and Biomedical Sciences, University of Yaounde, Cameroon.
Ngu
J L
JL
McKerrow
J L
JL
Seigel
J
J
Sun
E
E
Barr
P J
PJ
Bathurst
I
I
Morgan
G
G
Nkenfou
C
C
Asonganyi
T
T
Lando
G
G
D000818:Animals; D000947:Antigens, Helminth / Q000276:immunology*; D002448:Cell Adhesion; D018076:DNA, Complementary / Q000032:analysis; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D007962:Leukocytes / Q000502:physiology; D051379:Mice; D017181:Onchocerca volvulus / Q000276:immunology*; D009855:Onchocerciasis / Q000175:diagnosis; D011817:Rabbits; D011994:Recombinant Proteins / Q000276:immunology; D012441:Saccharomyces cerevisiae / Q000235:genetics; D012680:Sensitivity and Specificity
D000947:Antigens, Helminth; D018076:DNA, Complementary; D011994:Recombinant Proteins