title: Interleukin-2 production by cultured human granulosa cells.
abstract: OBJECTIVE
  To determine whether cultured human granulosa cells (GC) produce Interleukin (IL)-2 and whether this GC IL-2 production may be regulated by human chorionic gonadotropin (hCG).
  
  
  METHODS
  Human GC derived from preovulatory follicles during in vitro fertilization (IVF) cycles were cultured in the absence or presence of hCG. Interleukin-2 was measured in tissue-culture medium by an enzyme-linked immunosorbent assay (ELISA) system.
  
  
  RESULTS
  Cultured GCs were demonstrated to produce IL-2 in vitro. Moreover, a positive, but not statistically significant, dose-response effect was demonstrated between culture supernatant IL-2 levels and hCG concentration.
  
  
  CONCLUSIONS
  Our data indicate that human GC produce IL-2 and that this production might be regulated by hCG. These results lend credence to the theory that IL-2 might be involved in the events leading to ovarian hyperstimulation syndrome (OHSS).
authors.affiliation: Department of Obstetrics and Gynecology, Rabin Medical Center-Campus Golda (Hasharon Hospital), Petah Tikva, Israel.
authors.forename: R
authors.initials: R
authors.lastname: Orvieto
authors.forename: A R
authors.initials: AR
authors.lastname: Genazzani
authors.forename: F
authors.initials: F
authors.lastname: Petraglia
authors.forename: A
authors.initials: A
authors.lastname: Schwartz
authors.forename: M
authors.initials: M
authors.lastname: Shelef
authors.forename: Z
authors.initials: Z
authors.lastname: Ben-Rafael
mesh_terms: D002478:Cells, Cultured; D006063:Chorionic Gonadotropin / Q000008:administration & dosage / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D004797:Enzyme-Linked Immunosorbent Assay; D004958:Estradiol / Q000378:metabolism; D005260:Female; D005307:Fertilization in Vitro; D005500:Follow-Up Studies; D006107:Granulosa Cells / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D006801:Humans; D007376:Interleukin-2 / Q000096:biosynthesis*; D016471:Ovarian Hyperstimulation Syndrome / Q000209:etiology / Q000378:metabolism / Q000517:prevention & control; D011374:Progesterone / Q000378:metabolism
chemical_list: D006063:Chorionic Gonadotropin; D007376:Interleukin-2; D011374:Progesterone; D004958:Estradiol