Rabbit IgG distribution in skin, spinal cord and DRG following systemic injection in rat.
In order to determine the distribution of antibodies such as anti-NGF following systemic injection in neonates, immunocytochemical techniques were used to examine the localization of rabbit IgG in rat skin, DRG, and spinal cord after treatments with normal rabbit serum or purified rabbit IgG. Daily subcutaneous injections beginning on postnatal day 2 or on day 15 were given for three days. On the fourth day the animals were sacrificed and tissues were processed for rabbit IgG-IR. In the dorsal and ventral spinal cord, staining intensities suggest a substantial increase in the blood-brain barrier during the first two weeks after birth. Staining intensity in the epidermis of the glabrous skin from the hindpaw was substantially lower than in the adjacent dermis. In addition, IgG infrequently accumulated intracellularly in intensely stained patches in the epidermis. IgG was also able to reach relatively high intracellular concentrations in a small number of sensory neurons. The IgG staining pattern in the skin was similar when anti-NGF itself was administered to the animals. The results are discussed in the context of the effects of anti-NGF on the development of nociceptive afferents.
Tonra
J R
JR
Department of Physiology and Biophysics, SUNY at Stony Brook 11794, USA.
Mendell
L M
LM
D000818:Animals; D004817:Epidermis / Q000737:chemistry / Q000276:immunology / Q000378:metabolism; D005260:Female; D005727:Ganglia, Spinal / Q000737:chemistry / Q000276:immunology* / Q000378:metabolism; D007106:Immune Sera / Q000008:administration & dosage; D007074:Immunoglobulin G / Q000008:administration & dosage* / Q000378:metabolism*; D007150:Immunohistochemistry; D007279:Injections, Subcutaneous; D009414:Nerve Growth Factors / Q000276:immunology; D011817:Rabbits; D051381:Rats; D017207:Rats, Sprague-Dawley; D012867:Skin / Q000737:chemistry / Q000276:immunology* / Q000378:metabolism; D013116:Spinal Cord / Q000737:chemistry / Q000276:immunology* / Q000378:metabolism; D013194:Staining and Labeling; D014018:Tissue Distribution / Q000276:immunology
D007106:Immune Sera; D007074:Immunoglobulin G; D009414:Nerve Growth Factors