title: Differential internalisation of mGluR1 splice variants in response to agonist and phorbol esters in permanently transfected BHK cells.
abstract: The internalisation of metabotropic glutamate receptor (mGluR1alpha) and its splice variant (mGluR1beta), in response to agonist and phorbol esters (PMA), has been studied. Both mGluR1alpha and mGluR1beta exhibit a similar rate of internalisation following PMA treatment, with a shift in their distribution from plasma membrane to endosome-enriched membrane fractions. Agonist challenge however caused a rapid loss, within 5-10 min, of mGluR1beta but not mGluR1alpha from the cell surface. These results show that the two forms of mGluR1 show different internalisation responses to agonist and suggest that the C-terminal region of the molecule plays an important role in this phenomenon.
authors.affiliation: Medical Research Council Anatomical Neuropharmacology Unit, Oxford, UK.
authors.forename: F
authors.initials: F
authors.lastname: Ciruela
authors.forename: R A
authors.initials: RA
authors.lastname: McIlhinney
mesh_terms: D017398:Alternative Splicing; D000818:Animals; D002460:Cell Line; D002462:Cell Membrane / Q000378:metabolism; D006224:Cricetinae; D011992:Endosomes / Q000378:metabolism; D014644:Genetic Variation; D007668:Kidney; D007700:Kinetics; D018094:Receptors, Metabotropic Glutamate / Q000096:biosynthesis* / Q000378:metabolism; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis / Q000378:metabolism; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology; D014162:Transfection
chemical_list: D018094:Receptors, Metabotropic Glutamate; D011993:Recombinant Fusion Proteins; C104077:metabotropic glutamate receptor type 1; D013755:Tetradecanoylphorbol Acetate