title: A magnetic colloid system for isolation of rare cells from blood for fish analysis.
abstract: To improve cell recovery of trophoblast and nucleated red blood cells from maternal blood for diagnosis of chromosomal abnormalities, we have investigated the use of a magnetic sorting system that utilizes a ferrofluid. The main advantage of this system is that the beads used are small enough to allow visualization of chromosome-specific sequences by fluorescence in situ hybridization (FISH). The ferrofluid was validated using MAb340, a trophoblast-specific antibody, and anti-CD71, used to sort for nucleated red blood cells. Antigen-positive cells could be efficiently sorted from a 1000-fold excess of antigen-negative cells and easily stained by FISH. We are currently evaluating its use on maternal blood samples.
authors.affiliation: Department of Obstetrics and Gynaecology, City Hospital, Nottingham, U.K.
authors.forename: B
authors.initials: B
authors.lastname: Martin
authors.forename: J
authors.initials: J
authors.lastname: Bruce
authors.forename: N
authors.initials: N
authors.lastname: Smith
authors.forename: D
authors.initials: D
authors.lastname: Liu
authors.forename: L
authors.initials: L
authors.lastname: Durrant
mesh_terms: D001773:Blood Cells; D002452:Cell Count; D002469:Cell Separation; D003102:Colloids; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D008280:Magnetics; D008297:Male; D008863:Microspheres; D014407:Tumor Cells, Cultured
chemical_list: D003102:Colloids