title: Secretory immunoglobulin A blocks hypoxia-augmented bacterial passage across Madin-Darby canine kidney cell monolayers.
abstract: OBJECTIVE
  To study the relative impact of previous hypoxic exposure and the addition of secretory immunoglobulin A (IgA) on bacterial translocation.
  
  
  METHODS
  In vitro randomized experimental study.
  
  
  METHODS
  Transfected Madin-Darby canine kidney epithelial cells were grown as monolayers in a two-chamber tissue culture system. Stationary growth phase Escherichia coli M14 were inoculated in the apical chamber with medium or medium containing polymeric secretory IgA. Tissue culture dishes were then placed in a 21 or 5% O2 incubator environment for 90 minutes followed by a 21% O2 environment. Medium from the basal compartment was then obtained at timed intervals for bacterial culture.
  
  
  RESULTS
  Bacterial translocation increased with time in co-culture. Previous hypoxic exposure augmented translocation across the monolayers. The addition of IgA blocked translocation under both normoxic and hypoxic conditions.
  
  
  CONCLUSIONS
  Secretory IgA is important in mucosal defense under both normal and shock conditions.
authors.affiliation: Department of Surgery, Wayne State University, Detroit, Michigan 48201, USA.
authors.forename: L N
authors.initials: LN
authors.lastname: Diebel
authors.forename: D M
authors.initials: DM
authors.lastname: Liberati
authors.forename: W J
authors.initials: WJ
authors.lastname: Brown
authors.forename: S A
authors.initials: SA
authors.lastname: Dulchavsky
authors.forename: T M
authors.initials: TM
authors.lastname: Painter
authors.forename: C A
authors.initials: CA
authors.lastname: Diglio
authors.forename: P C
authors.initials: PC
authors.lastname: Montgomery
mesh_terms: D000818:Animals; D018988:Bacterial Translocation / Q000276:immunology / Q000502:physiology*; D002478:Cells, Cultured; D004285:Dogs; D004926:Escherichia coli / Q000254:growth & development / Q000502:physiology*; D000860:Hypoxia / Q000382:microbiology / Q000503:physiopathology*; D007070:Immunoglobulin A; D007071:Immunoglobulin A, Secretory / Q000502:physiology*; D007668:Kidney / Q000166:cytology / Q000382:microbiology
chemical_list: D007070:Immunoglobulin A; D007071:Immunoglobulin A, Secretory