Evaluation of non-invasive clinical samples in chronic chlamydial prostatitis by using in situ hybridization.
Seventy-eight non-invasive prostate specimens collected from patients with chronic non-bacterial prostatitis were evaluated by in situ hybridization (IH) for evidence of Chlamydia trachomatis. Intracellular Chlamydia bodies were detected in 18 of 78 cases (20.6%). Homogeneous blue-black bodies in the cellular cytoplasm were accepted as in situ positive. Chlamydial antigen detected by enzyme immuno assay (EIA) was positive in 12 cases (13.7%), but only nine of them were positive by IH. Our study confirms previous reports implicating C. trachomatis as an aetiological agent in chronic non-bacterial prostatitis, and underscores the applicability of DNA probes for detection and identification of C. trachomatis in prostatic materials.
Gümüş
B
B
Department of Urology, Faculty of Medicine, University of Celal Bayer, Manisa, Turkey.
Sengil
A Z
AZ
Solak
M
M
Fistik
T
T
Alibey
E
E
Cakmak
E A
EA
Yeter
M
M
D000328:Adult; D002690:Chlamydia Infections / Q000175:diagnosis*; D002692:Chlamydia trachomatis / Q000302:isolation & purification*; D002908:Chronic Disease; D006801:Humans; D007124:Immunoenzyme Techniques; D017403:In Situ Hybridization; D008297:Male; D011472:Prostatitis / Q000382:microbiology*