Comparison of bone marrow toxicity of medium-chain and long-chain triglyceride emulsions: an in vitro study in humans.
BACKGROUND
In this study, we evaluated the in vitro bone marrow toxicity of two lipid emulsions containing either long-chain triglycerides (LCT) or a mixture of medium-chain triglycerides (MCT) and LCT.


METHODS
Bone marrow cells were obtained from six healthy subjects and were cultured for 14 days after a 24-hour preincubation with various concentrations (from 0 to 10 mg/mL) of LCT- and LCT/MCT-based lipid emulsions.


RESULTS
Compared with controls (no preincubation with lipid emulsion), both lipid emulsions significantly inhibited by 50% to 70% colony formation of all the human bone marrow cells cultured from a triglyceride concentration of 0.5 mg/mL (p < .05). Erythroid burst-forming unit (BFU-E) formation was significantly more inhibited with LCT/MCT emulsion than with LCT emulsion (p < .05). The inhibition of granulocyte-macrophage colony-forming unit (GM-CFU) and mixed granulocyte-erythrocyte-monocyte-megakaryocyte colony-forming unit (GEMM-CFU) formation did not significantly differ with the two emulsions.


CONCLUSIONS
Both LCT- and LCT/MCT-based lipid emulsions strongly inhibit colony formation by human bone marrow cells. BFU-E colony formation is more sensitive to LCT/MCT inhibition than to LCT.
Beau
P
P
Gastroenterology and Nutritional Support Unit, University Hospital, Poitiers, France.
Mannant
P R
PR
Pelletier
D
D
Brizard
A
A
D000328:Adult; D001854:Bone Marrow Cells / Q000187:drug effects*; D016923:Cell Death / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D004655:Emulsions; D015672:Erythroid Precursor Cells / Q000187:drug effects; D005260:Female; D006412:Hematopoietic Stem Cells / Q000187:drug effects; D006801:Humans; D066298:In Vitro Techniques; D008297:Male; D013997:Time Factors; D014280:Triglycerides / Q000737:chemistry* / Q000494:pharmacology*
D004655:Emulsions; D014280:Triglycerides