Reduced kidney branched chain aminotransferase expression in puromycin aminonucleoside-induced nephrotic syndrome.
Injection of puromycin aminonucleoside to rats induces nephrotic syndrome characterized by hypoalbuminemia, proteinuria and hypercholesterolemia. In these rats, a low protein diet (6% casein diet) increased serum albumin by 26.3%, decreased proteinuria by 39% and reduced total cholesterol by 32%. Branched chain aminotransferase activity in kidney mitochondria of nephrotic rats fed 20 or 6% casein diet decreased by 30 and 24% with respect to their pair-fed groups and it was not modified by the protein content of the diet. Mitochondrial branched chain aminotransferase mRNA expression decreased by 67.3 and 72.5% in nephrotic rats fed 20 and 6% casein diet in comparison to their pair-fed groups. Total serum branched chain amino acids concentration (leucine, isoleucine, valine) in nephrotic rats was 30% higher than their pair-fed groups and it was associated with a decrease in the branched chain aminotransferase activity and mRNA expression suggesting that the catabolism of branched chain amino acid is reduced to conserve body nitrogen.
Ascencio
C
C
Departamento de Fisiología de la Nutrición, Instituto Nacional de la Nutrición Salvador Zubiran, México, D.F., México.
Torres
N
N
Sandoval
R L
RL
Cruz
C
C
Pedraza-Chaverri
J
J
Tovar
A R
AR
D000818:Animals; D001835:Body Weight; D004044:Dietary Proteins / Q000008:administration & dosage; D004435:Eating; D007668:Kidney / Q000201:enzymology*; D008297:Male; D009404:Nephrotic Syndrome / Q000139:chemically induced / Q000201:enzymology*; D011692:Puromycin Aminonucleoside / Q000633:toxicity; D012333:RNA, Messenger / Q000032:analysis; D051381:Rats; D017208:Rats, Wistar; D000637:Transaminases / Q000235:genetics / Q000378:metabolism*
D004044:Dietary Proteins; D012333:RNA, Messenger; D011692:Puromycin Aminonucleoside; D000637:Transaminases; C019798:branched-chain-amino-acid transaminase