title: Pregnancy after immature oocyte donation and intracytoplasmic sperm injection.
abstract: OBJECTIVE
  To report a case of pregnancy from in vitro-matured primary oocytes fertilized by ICSI. The pregnancy occurred in a woman who was in an oocyte donation program; the woman's husband had normal sperm parameters.
  
  
  METHODS
  Case report.
  
  
  METHODS
  Private general hospital affiliated with a university hospital.
  
  
  METHODS
  A recipient with premature ovarian failure, a recipient's husband with normal sperm, and a pregnant woman who donated her oocytes.
  
  
  METHODS
  Aspiration of immature oocytes during cesarean section, in vitro culture for maturation, ICSI of matured oocytes, coculture of fertilized oocytes.
  
  
  METHODS
  Fertilization of oocytes by ICSI, and cleavage of embryos by Vero cell coculture.
  
  
  RESULTS
  Two of seven immature oocytes became metaphase II oocytes, and both were fertilized by ICSI. The two zygotes were cocultured on Vero cells to become grade 1 two-cell embryos. Pregnancy was obtained after transfer.
  
  
  CONCLUSIONS
  More studies are necessary to clarify whether ICSI can increase the fertilization rate of in vitro-matured primary oocytes, and to clarify the role of coculture in fertilization.
authors.affiliation: Department of Obstetrics and Gynecology, Shin Kong Wu Ho-Su Memorial Hospital, Taipei, Taiwan, Republic of China.
authors.forename: J L
authors.initials: JL
authors.lastname: Hwang
authors.forename: Y H
authors.initials: YH
authors.lastname: Lin
authors.forename: Y L
authors.initials: YL
authors.lastname: Tsai
mesh_terms: D000328:Adult; D002478:Cells, Cultured; D003593:Cytoplasm; D005260:Female; D005307:Fertilization in Vitro / Q000379:methods*; D006801:Humans; D008845:Microinjections; D018587:Oocyte Donation / Q000379:methods*; D009865:Oocytes / Q000254:growth & development*; D011247:Pregnancy; D013396:Suction