Identification of an alpha-D-Manp-(1-->8)-Kdo disaccharide in the inner core region and the structure of the complete core region of the Legionella pneumophila serogroup 1 lipopolysaccharide.
A disaccharide alpha-D-mannopyranosyl-(1-->8)-3-deoxy-D-manno-octulosonic acid [alpha-D-Manp-(1-->8)-Kdo] was released by mild acid degradation of Legionella pneumophila serogroup 1 (strain Philadelphia 1) lipopolysaccharide (LPS) and identified using NMR spectroscopy and GLC-MS of derived products. These data, together with methylation analysis of the native LPS and previously reported data [Y.A. Knirel, H. Moll, and U. Zähringer, Carbohydr. Res., 293 (1996) 223-234], allowed elucidation of the complete core region of the LPS as having the following nonasaccharide structure: [Sequence: see text]
Moll
H
H
Forschungszentrum Borstel, Zentrum für Medizin und Biowissenschaften, Borstel, Germany.
Knirel
Y A
YA
Helbig
J H
JH
Zähringer
U
U
D002236:Carbohydrate Conformation; D002240:Carbohydrate Sequence; D004187:Disaccharides / Q000032:analysis* / Q000737:chemistry; D016952:Legionella pneumophila / Q000737:chemistry*; D008070:Lipopolysaccharides / Q000737:chemistry; D009682:Magnetic Resonance Spectroscopy; D008362:Mannosides / Q000737:chemistry; D008969:Molecular Sequence Data; D015394:Molecular Structure; D011135:Polysaccharides, Bacterial / Q000032:analysis / Q000737:chemistry*; D012699:Serology
D004187:Disaccharides; D008070:Lipopolysaccharides; D008362:Mannosides; D011135:Polysaccharides, Bacterial