Analysis of gene expression in the organ of Corti revealed by single-cell RT-PCR.
Many genes encoding proteins which are expressed in the auditory periphery have been identified in the last years. With single-cell reverse transcription-polymerase chain reaction (RT-PCR), the molecular analysis of gene expression can be done on the single-cell level. Furthermore a single-cell RT-PCR experiment can be combined with the electrophysiological characterization of an individual cell. The combination of these two methods will lead to a better understanding of how functional properties of neurons are controlled by the expression of complex proteins.
Glowatzki
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Department of Otolaryngology, University of Tübingen, Germany. Elisabeth.Glowatzki@Uni-Tuebingen.de
D000818:Animals; D018076:DNA, Complementary / Q000235:genetics; D004594:Electrophysiology; D015870:Gene Expression; D066298:In Vitro Techniques; D009925:Organ of Corti / Q000166:cytology* / Q000378:metabolism* / Q000502:physiology; D016133:Polymerase Chain Reaction / Q000379:methods*; D012333:RNA, Messenger / Q000235:genetics; D018091:Receptors, AMPA / Q000235:genetics
D018076:DNA, Complementary; D012333:RNA, Messenger; D018091:Receptors, AMPA