Analysis of protein aggregates by combination of cross-linking reactions and chromatographic separations.
Chemical cross-linking provides a method that covalently bridges near-neighbour associations within proteins and protein aggregates. Combined with chromatographic separations and protein-chemical methods, it may be used to localize and to investigate three-dimensional relations as present under natural conditions. This paper reviews the chemistry and application of cross-linking reagents and the development of combination experimental approaches in view of chromatographic separations and cross-linking reactions. Investigations of homooligomeric and heterooligomeric protein associations as well as conformational analysis are presented.
Löster
K
K
Institut für Molekularbiologie und Biochemie, Freie Universität Berlin, Berlin-Dahlem, Germany.
Josić
D
D
D002853:Chromatography, Liquid / Q000379:methods*; D003432:Cross-Linking Reagents / Q000737:chemistry*; D005634:Fructose-Bisphosphate Aldolase / Q000737:chemistry; D006360:Heat-Shock Proteins / Q000737:chemistry; D016023:Integrins / Q000737:chemistry; D011506:Proteins / Q000737:chemistry*
D003432:Cross-Linking Reagents; D006360:Heat-Shock Proteins; D016023:Integrins; D011506:Proteins; D005634:Fructose-Bisphosphate Aldolase