title: Involvement of MAP-kinases and -phosphatases in uptake and intracellular replication of Listeria monocytogenes in J774 macrophage cells.
abstract: In this study we show that protein tyrosine kinases and also protein tyrosine phosphatases are involved in the uptake of Listeria monocytogenes by J774 macrophages to a different extent than in the uptake of inert latex beads. In addition, protein tyrosine kinases are necessary for the intracellular growth and survival of L. monocytogenes. The expression of the MAP kinase phosphatase MKP-1, a protein tyrosine phosphatase, is induced upon infection, and phagocytosis of L. monocytogenes by J774 cells overexpressing the MKP-1 protein is reduced compared to control cells. The decreased phagocytosis of L. monocytogenes as a result of the MKP-1 overexpression in J774 macrophages suggests that the activation of the MAP kinase(s) ERK-1 and/or ERK-2 is an essential requirement for the uptake of L. monocytogenes by J774 macrophages.
authors.affiliation: Theodor-Boveri-Institut für Biowissenschaften, Lehrstuhl für Mikrobiologie, Universität Würzburg, Germany. skuegler@borcim.wustl.edu
authors.forename: S
authors.initials: S
authors.lastname: Kügler
authors.forename: S
authors.initials: S
authors.lastname: Schüller
authors.forename: W
authors.initials: W
authors.lastname: Goebel
mesh_terms: D000818:Animals; D017871:Calcium-Calmodulin-Dependent Protein Kinases / Q000235:genetics / Q000378:metabolism*; D004791:Enzyme Inhibitors / Q000494:pharmacology; D005786:Gene Expression Regulation / Q000276:immunology; D019833:Genistein / Q000494:pharmacology; D007840:Latex; D008089:Listeria monocytogenes / Q000254:growth & development*; D008088:Listeriosis / Q000201:enzymology*; D008264:Macrophages / Q000201:enzymology / Q000276:immunology / Q000382:microbiology*; D051379:Mice; D008863:Microspheres; D010587:Phagocytosis / Q000187:drug effects / Q000276:immunology; D014176:Protein Biosynthesis; D017027:Protein Tyrosine Phosphatases / Q000235:genetics / Q000378:metabolism*; D014638:Vanadates / Q000494:pharmacology
chemical_list: D004791:Enzyme Inhibitors; D007840:Latex; D014638:Vanadates; D019833:Genistein; D017871:Calcium-Calmodulin-Dependent Protein Kinases; D017027:Protein Tyrosine Phosphatases