Cloning of two novel human importin-alpha subunits and analysis of the expression pattern of the importin-alpha protein family.
The import of many proteins into the nucleus is mediated by the importin-alpha/beta heterodimer. While only one importin-beta gene has been found, several forms of importin-alpha have been described. In addition to the three human importin-alphas already identified, we report here the primary structure of two new human importin-alpha proteins. The five known human importin-alpha subunits can be classified into three subfamilies that appear conserved in higher eukaryotic organisms. We show by immunoblotting that the different importin-alpha subfamilies are expressed in a variety of human tissues and mammalian cell lines.
Köhler
M
M
Franz Volhard Clinic, Humboldt University of Berlin, Germany.
Ansieau
S
S
Prehn
S
S
Leutz
A
A
Haller
H
H
Hartmann
E
E
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D018076:DNA, Complementary; D015870:Gene Expression; D006367:HeLa Cells; D006801:Humans; D015151:Immunoblotting; D019169:Jurkat Cells; D028884:Karyopherins; D008969:Molecular Sequence Data; D009687:Nuclear Proteins / Q000096:biosynthesis* / Q000145:classification / Q000235:genetics / Q000276:immunology; D012333:RNA, Messenger; D011817:Rabbits; D017386:Sequence Homology, Amino Acid; D014018:Tissue Distribution; D014407:Tumor Cells, Cultured
D018076:DNA, Complementary; D028884:Karyopherins; D009687:Nuclear Proteins; D012333:RNA, Messenger