title: Early expression of a beta1-adrenergic receptor and catecholamines in Xenopus oocytes and embryos.
abstract: From a Xenopus stage 11 cDNA library, we have cloned a gene, termed X-beta1AR, whose sequence is highly homologous to that of the human beta1-adrenergic receptor. As shown by RT-PCR assay, X-beta1AR RNA is present in the mature oocyte, decreases after fertilization up to stage 6 and then gradually increases during gastrulation. Binding studies performed with radiolabeled ligands reveal that X-beta1AR RNA is translated into the receptor protein. Furthermore, noradrenaline and adrenaline are also detected in oocytes and early embryos. The concomitant presence of beta1-adrenergic receptors and catecholamines suggest that this ligand-receptor couple could play a role in the very early stages of embryonic development.
authors.affiliation: Unité U-397 INSERM, CHU Rangueil, Toulouse, France.
authors.forename: E
authors.initials: E
authors.lastname: Devic
authors.forename: L
authors.initials: L
authors.lastname: Paquereau
authors.forename: R
authors.initials: R
authors.lastname: Steinberg
authors.forename: D
authors.initials: D
authors.lastname: Caput
authors.forename: Y
authors.initials: Y
authors.lastname: Audigier
mesh_terms: D000818:Animals; D001483:Base Sequence; D002395:Catecholamines / Q000378:metabolism*; D018076:DNA, Complementary / Q000235:genetics; D015870:Gene Expression; D018507:Gene Expression Regulation, Developmental; D008969:Molecular Sequence Data; D009865:Oocytes; D010869:Pindolol / Q000031:analogs & derivatives / Q000378:metabolism; D011869:Radioligand Assay; D018342:Receptors, Adrenergic, beta-1 / Q000235:genetics* / Q000378:metabolism; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid; D014982:Xenopus laevis / Q000196:embryology* / Q000235:genetics
chemical_list: D002395:Catecholamines; D018076:DNA, Complementary; D018342:Receptors, Adrenergic, beta-1; C047975:cyanopindolol; D010869:Pindolol