Development of a gelatin particle agglutination reagent for measles antibody assay.
A new agglutination test that utilizes gelatin particles as the carrier of measles antigen was developed and used to evaluate immune status to measles virus infection. The particle agglutination (PA) reagent reacted with monoclonal antibodies against two major proteins of measles virus, the hemagglutinin (H) and fusion (F) proteins. Children were followed individually for ten years for measles antibody. Results showed that the PA test was as sensitive and specific as the plaque neutralization test. The procedure is simple and rapid. No prior treatment of specimens is needed, and the test is completed in a single reaction. The PA test therefore can be used for diagnoses and epidemiologic surveys of measles virus infection.
Sato
T A
TA
Department of Virus Diseases and Vaccine Control, National Institute of Infectious Diseases, Tokyo, Japan.
Miyamura
K
K
Sakae
K
K
Kobune
F
F
Inouye
S
S
Fujino
R
R
Yamazaki
S
S
D000372:Agglutination Tests / Q000379:methods*; D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D000914:Antibodies, Viral / Q000032:analysis* / Q000276:immunology; D002648:Child; D002675:Child, Preschool; D002522:Chlorocebus aethiops; D005500:Follow-Up Studies; D005780:Gelatin; D006389:Hemagglutinins, Viral / Q000276:immunology; D006801:Humans; D007202:Indicators and Reagents; D008457:Measles / Q000276:immunology*; D009500:Neutralization Tests; D014709:Vero Cells; D014760:Viral Fusion Proteins / Q000276:immunology
D000911:Antibodies, Monoclonal; D000914:Antibodies, Viral; D006389:Hemagglutinins, Viral; D007202:Indicators and Reagents; D014760:Viral Fusion Proteins; C032397:hemagglutinin protein G, measles virus; D005780:Gelatin