Development of vasa vasorum in the arterially implanted autovein bypass graft and its anastomosis in the dog.
Using stereomicroscopy, light microscopy, and scanning electron microscopy, we investigated the development of vasa vasorum in the proliferated neointima of the autovein graft and its anastomoses implanted in the canine femoral artery against a background of poor distal runoff. In the stereomicroscopic examination, a microfil silicone rubber compound (MF) was injected transluminally or via perivascular vasa, and the vascular specimen was prepared for clearing by immersion in a methyl-salicylate solution. Vessel interstices filled with MF were found adjacent to the suture materials within 5 days of grafting. Fourteen days after implantation, luminally originating vasa vasorum were often visible in the neointima along the suture line and distributed into the media and adventitia connecting to the original vasa vasorum. At 6 months or more after grafting, many orifices of luminally originating vasa vasorum were seen along the suture line of both proximal end-to-end and distal end-to-side anastomoses and distributed into the thickened neointima forming a vasa network when the neointima had proliferated to over 250 microm in depth. On the other hand, some clefts filled with MF were found in mural thrombi deposited on the vascular sinus of the graft within 5 days, and these appeared to be one of the sources of luminally originating vasa vasorum on the graft distant from the suture line. Moreover, the development of numerous vasa vasorum was constantly demonstrated in the neointima when it had proliferated to over 250 microm in depth.
Ohta
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O
Second Department of Surgery, Faculty of Medicine, University of the Ryukyus, Okinawa, Japan.
Kusaba
A
A
D000714:Anastomosis, Surgical; D000818:Animals; D001157:Arterial Occlusive Diseases / Q000601:surgery; D001783:Blood Flow Velocity; D002455:Cell Division; D004195:Disease Models, Animal; D004285:Dogs; D005260:Female; D005263:Femoral Artery / Q000473:pathology / Q000503:physiopathology / Q000601:surgery*; D005268:Femoral Vein / Q000473:pathology / Q000503:physiopathology / Q000637:transplantation*; D006083:Graft Occlusion, Vascular / Q000209:etiology / Q000473:pathology / Q000503:physiopathology; D008297:Male; D008855:Microscopy, Electron, Scanning; D009389:Neovascularization, Pathologic; D014182:Transplantation, Autologous; D017539:Tunica Intima / Q000648:ultrastructure; D014651:Vasa Vasorum / Q000473:pathology*