DNA amplification polymorphisms of Mucor piriformis.
Mucor piriformis can cause postharvest decay in various fruits and vegetables stored at low temperatures. Thirty isolates of this fungus, collected from infected fruit, were subjected to random amplified polymorphic DNA (RAPD) analysis. Seven different 10-bp primers were used to determine the type and extent of intraspecific genetic polymorphisms. Nineteen composite amplification types were identified, indicating a higher degree of variability than found in previous isoenzyme studies. Numerical analysis with the UPGMA technique revealed three clusters, which correlated with the mating competency of the isolates or their place of origin. These results demonstrate that RAPD analysis can identify isolates and subspecific populations of M. piriformis.
Papp
T
T
Department of Microbiology, Attila József University, Szeged, Hungary.
Vágvölgyi
C
C
Kerényi
Z
Z
Nagy
A
A
Michailides
T J
TJ
D017931:DNA Primers; D004271:DNA, Fungal / Q000235:genetics*; D005819:Genetic Markers; D009089:Mucor / Q000145:classification / Q000235:genetics* / Q000502:physiology; D011110:Polymorphism, Genetic; D019105:Random Amplified Polymorphic DNA Technique; D012098:Reproduction; D013045:Species Specificity
D017931:DNA Primers; D004271:DNA, Fungal; D005819:Genetic Markers