title: Regulation of mouse immuno-responses by a natural suppressor cell clone from bone marrow.
abstract: Natural suppressor (NS) activity is mediated by several kinds of cell lineages in bone marrow. We demonstrated that a NS cell, clone 7-31, was obtained after limiting dilution with bone marrow culture supplemented with WEHI supernatant. Clone 7-31, was capable of non-specific suppression of the generation of cytotoxic T lymphocytes without major histocompatibility complex restriction. Suppression of cytotoxic thymus-dependent lymphocyte (CTL) generation was also induced with a cell-free supernatant from the 7-31 cells. Interleukin-2 (IL-2) containing concanavallin-A-conditioned medium could not reverse the suppression. The supernatant did not inhibit the proliferation of IL-2-dependent CTLL-2 cells and rapidly growing tumour cells and fibroblasts. Thus, this bone marrow suppressor cell produces a soluble factor that inhibits the generation of CTL in vitro, and prolongs the acceptance of skin allografts in in vivo.
authors.affiliation: Department of Surgery, University of Tokyo, Japan.
authors.forename: H
authors.initials: H
authors.lastname: Yanagië
authors.forename: Z
authors.initials: Z
authors.lastname: Chen
authors.forename: Y
authors.initials: Y
authors.lastname: Takeda
authors.forename: H
authors.initials: H
authors.lastname: Sugiyama
authors.forename: M
authors.initials: M
authors.lastname: Sekiguchi
authors.forename: M
authors.initials: M
authors.lastname: Eriguchi
mesh_terms: D000818:Animals; D000941:Antigens / Q000276:immunology; D001854:Bone Marrow Cells / Q000276:immunology*; D002999:Clone Cells / Q000187:drug effects / Q000276:immunology; D003601:Cytotoxicity Tests, Immunologic; D005347:Fibroblasts / Q000166:cytology / Q000276:immunology; D006085:Graft Survival / Q000276:immunology; D007165:Immunosuppression Therapy; D007376:Interleukin-2 / Q000494:pharmacology; D007694:Killer Cells, Natural / Q000166:cytology / Q000276:immunology; D008213:Lymphocyte Activation / Q000187:drug effects / Q000276:immunology*; D051379:Mice; D008809:Mice, Inbred C3H; D008810:Mice, Inbred C57BL; D008811:Mice, Inbred DBA; D008934:Mitogens / Q000494:pharmacology; D016038:Skin Transplantation / Q000276:immunology; D013602:T-Lymphocytes, Cytotoxic / Q000166:cytology / Q000276:immunology; D014184:Transplantation, Homologous; D014407:Tumor Cells, Cultured / Q000276:immunology
chemical_list: D000941:Antigens; D007376:Interleukin-2; D008934:Mitogens