title: Zanhasaponins A and B, antiphospholipase A2 saponins from an antiinflammatory extract of Zanha africana root bark.
abstract: A MeOH extract from Z. africana was examined for topical antiinflammatory activity and proved to be active against arachidonic acid (AA) acute edema, 12-O-tetradecanoylphorbol 13-acetate (TPA)-induced chronic inflammation, and oxazolone delayed-type hypersensitivity in mice. The extract also showed significant inhibitory activity of Naja naja phospholipase A2 when a polarographic method was used. Two oleanane-type triterpene saponins, zanhasaponins A (1) and B (2), and the cyclitol pinitol (4), isolated from the extract, were active as inhibitors of PLA2. A further saponin, zanhasaponin C (3) was inactive in this assay.
authors.affiliation: Departament de Farmacologia, Facultat de Farmàcia, Universitat de València, Spain.
authors.forename: M J
authors.initials: MJ
authors.lastname: Cuéllar
authors.forename: R M
authors.initials: RM
authors.lastname: Giner
authors.forename: M C
authors.initials: MC
authors.lastname: Recio
authors.forename: M J
authors.initials: MJ
authors.lastname: Just
authors.forename: S
authors.initials: S
authors.lastname: Máñez
authors.forename: M
authors.initials: M
authors.lastname: Cerdá
authors.forename: K
authors.initials: K
authors.lastname: Hostettmann
authors.forename: J L
authors.initials: JL
authors.lastname: Ríos
mesh_terms: D000276:Adjuvants, Immunologic; D000818:Animals; D000893:Anti-Inflammatory Agents / Q000302:isolation & purification* / Q000494:pharmacology; D003877:Dermatitis, Contact / Q000188:drug therapy / Q000473:pathology; D004487:Edema / Q000139:chemically induced / Q000188:drug therapy / Q000473:pathology; D004791:Enzyme Inhibitors / Q000302:isolation & purification* / Q000494:pharmacology; D005260:Female; D051379:Mice; D010081:Oxazolone; D009195:Peroxidase / Q000037:antagonists & inhibitors; D010741:Phospholipases A / Q000037:antagonists & inhibitors*; D054467:Phospholipases A2; D012503:Saponins / Q000302:isolation & purification* / Q000494:pharmacology; D012867:Skin / Q000473:pathology; D013755:Tetradecanoylphorbol Acetate; D014315:Triterpenes / Q000302:isolation & purification* / Q000494:pharmacology
chemical_list: D000276:Adjuvants, Immunologic; D000893:Anti-Inflammatory Agents; D004791:Enzyme Inhibitors; D012503:Saponins; D014315:Triterpenes; C109949:zanhasaponin A; C109950:zanhasaponin B; D010081:Oxazolone; D009195:Peroxidase; D010741:Phospholipases A; D054467:Phospholipases A2; D013755:Tetradecanoylphorbol Acetate