title: High doses of hydroxyethyl starch and human albumin have similar effects on monocyte function and oncotic pressure.
abstract: The accumulation of hydroxyethyl starches (HES) in monocytes/macrophages has raised concern over their potential detrimental effects on host defences. We assessed prospectively the function of circulating monocytes isolated from patients treated with plasma exchange (PE) using HES. The study was carried out in the medical intensive care unit of a university hospital. Eight patients underwent PE for neurological disorders. Each patient underwent three PEs, 48 h apart. The total exchange volume was 4 L per PE. Only 4% human albumin was used for the first PE. In the second and third PEs, the plasma substitute was 2 L of HES (200,000/6%/0.62) and 2 L of albumin. Mononuclear cells were collected before and immediately after each PE and 48 h after the last PE. They were placed in suspension culture and incubated with lipopolysaccharide (LPS). Monocyte function was assessed in terms of procoagulant activity (PCA) and tumour necrosis factor alpha (TNF-alpha) production. LPS-stimulated PCA increased after the first PE (P < 0.05). Stimulated TNF-alpha production increased, but not significantly so. Similar effects were observed after the second and third PE (P < 0.05 for stimulated TNF-alpha). Values 48 h after the last PE were similar to those obtained before the second PE, suggesting that repeated infusions of HES had no detrimental effect on monocyte function. Furthermore, plasma oncotic pressure was preserved after PE with HES. These results support the partial replacement of costly human albumin with HES during repetitive PE, and suggest that HES might be a safe plasma expander in septic patients.
authors.affiliation: Intensive Care Unit, Hôpital Saint Antoine, Paris, France.
authors.forename: B
authors.initials: B
authors.lastname: Guidet
authors.forename: P
authors.initials: P
authors.lastname: Podevin
authors.forename: A
authors.initials: A
authors.lastname: Robert
authors.forename: P
authors.initials: P
authors.lastname: Chauvin
authors.forename: E
authors.initials: E
authors.lastname: Maury
authors.forename: G
authors.initials: G
authors.lastname: Offenstadt
mesh_terms: D000328:Adult; D000368:Aged; D000418:Albumins / Q000494:pharmacology*; D001779:Blood Coagulation Factors / Q000187:drug effects; D005260:Female; D006801:Humans; D006895:Hydroxyethyl Starch Derivatives / Q000494:pharmacology*; D008297:Male; D008875:Middle Aged; D009000:Monocytes / Q000187:drug effects* / Q000502:physiology; D009997:Osmotic Pressure; D010951:Plasma Exchange; D010952:Plasma Substitutes / Q000494:pharmacology*; D011446:Prospective Studies; D012709:Serum Albumin / Q000032:analysis; D014409:Tumor Necrosis Factor-alpha / Q000096:biosynthesis
chemical_list: D000418:Albumins; D001779:Blood Coagulation Factors; D006895:Hydroxyethyl Starch Derivatives; D010952:Plasma Substitutes; D012709:Serum Albumin; D014409:Tumor Necrosis Factor-alpha; C045071:leukocyte procoagulant activity