Culture with Matrigel inhibits development of mouse zygotes.
OBJECTIVE
It was reported that Matrigel improved hatching of mouse blastocysts produced in vitro from F1 hybrid-derived zygotes. We investigated whether Matrigel would be similarly beneficial with outbred strain-derived embryos, which exhibit a "two-cell" block similar to the developmental blocks of other species.


METHODS
Mouse embryo development was assessed with or without Matrigel in KSOM medium, which supports the development of blocking strain zygotes in vitro, and in human tubal fluid (HTF) medium, which normally does not but which is used for human IVF.


RESULTS
Matrigel severely inhibited the development of zygotes to blastocysts in KSOM and did not improve culture in HTF. There was no effect on development from the two-cell stage. We were not able to replicate the previous finding of Matrigel's beneficial effect on hatching of F1-derived zygotes.


CONCLUSIONS
Matrigel may be a deleterious addition to embryo culture or coculture systems.
Dawson
K M
KM
Loeb Medical Research Institute, Ottawa Civic Hospital, Ontario, Canada.
Baltz
J M
JM
Claman
P
P
D000818:Animals; D001755:Blastocyst / Q000187:drug effects; D001826:Body Fluids / Q000187:drug effects; D002455:Cell Division / Q000187:drug effects; D002478:Cells, Cultured; D003094:Collagen / Q000009:adverse effects / Q000032:analysis / Q000494:pharmacology*; D003470:Culture Media / Q000009:adverse effects / Q000032:analysis / Q000494:pharmacology*; D004338:Drug Combinations; D005314:Embryonic and Fetal Development / Q000187:drug effects* / Q000502:physiology; D005187:Fallopian Tubes / Q000187:drug effects; D005260:Female; D006801:Humans; D066298:In Vitro Techniques; D007797:Laminin / Q000009:adverse effects / Q000032:analysis / Q000494:pharmacology*; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D011509:Proteoglycans / Q000009:adverse effects / Q000032:analysis / Q000494:pharmacology*; D015053:Zygote / Q000187:drug effects* / Q000502:physiology
D003470:Culture Media; D004338:Drug Combinations; D007797:Laminin; D011509:Proteoglycans; C059485:matrigel; D003094:Collagen