title: Positive modulation of pepsinogen secretion by gastric acidity after vagal cholinergic stimulation.
abstract: Parallel increments of gastric acid and pepsinogen secretion generally occur after the application of cholinergic stimuli. However, it still remains to be established whether the changes in acid output associated with cholinergic stimulation play a role in regulation of the concomitant peptic secretory activity. In the present study, an anesthetized rat model was used for the evaluation of pepsinogen secretion in order to pursue a dual purpose: 1) to assess the relative functional relevance of direct and acid-dependent control exerted by cholinergic pathways on pepsinogen output; 2) to characterize the mechanisms through which changes in acidity within the stomach lumen may affect the peptic secretory activity of gastric mucosa. Bethanechol, 2-deoxy-D-glucose or electrical vagal stimulation caused parallel and atropine-sensitive increments of peptic and acid secretions. Omeprazole, a selective inhibitor of gastric H+:K+-adenosintriphosphatase, blocked the increase in acid but not pepsinogen secretion induced by bethanechol. However, 2-deoxy-D-glucose or electrical vagal stimulation failed to increase either pepsinogen or acid secretion in omeprazole-pretreated rats. When tested in animals pretreated with both omeprazole and physostigmine (a drug able to prevent the enzymatic breakdown of vagally released ACh through the blockade of acetylcholinesterase), 2-deoxy-D-glucose or electrical vagal stimulation significantly increased pepsinogen secretion without affecting acid secretion. In omeprazole-pretreated rats, perfusion of the gastric lumen with acid solutions caused a pH-dependent and atropine-sensitive increase in peptic output only when applied in combination with electrical vagal stimulation. Functional ablation of capsaicin-sensitive sensory neurons did not modify the gastric secretory responses induced by bethanechol or electrical vagal stimulation. However, after topical application of lidocaine to the gastric mucosal surface, bethanechol stimulated both peptic and acid outputs, whereas electrical vagal stimulation only evoked acid secretion without affecting basal peptic output. The present results indicate that the activation of muscarinic receptors by vagally released ACh is not sufficient by itself to stimulate pepsinogen secretion and that a facilitatory action mediated by acid secretion is necessary to allow an increment of peptic output in response to vagal cholinergic stimuli. It is suggested that such facilitatory input is driven to chief cells by local intramural reflexes that involve capsaicin-insensitive intrinsic nerves.
authors.affiliation: Division of Pharmacology and Chemotherapy, Department of Oncology, University of Pisa, Pisa, Italy.
authors.forename: C
authors.initials: C
authors.lastname: Blandizzi
authors.forename: R
authors.initials: R
authors.lastname: Colucci
authors.forename: D
authors.initials: D
authors.lastname: Carignani
authors.forename: G
authors.initials: G
authors.lastname: Lazzeri
authors.forename: M
authors.initials: M
authors.lastname: Del Tacca
mesh_terms: D000818:Animals; D018723:Bethanechol / Q000494:pharmacology; D002211:Capsaicin / Q000494:pharmacology; D004558:Electric Stimulation; D005744:Gastric Acid / Q000378:metabolism / Q000502:physiology*; D005745:Gastric Acidity Determination; D008012:Lidocaine / Q000494:pharmacology; D008297:Male; D010435:Pepsinogens / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar; D014630:Vagus Nerve / Q000502:physiology*
chemical_list: D010435:Pepsinogens; D018723:Bethanechol; D008012:Lidocaine; D002211:Capsaicin