title: Infection with rat cytomegalovirus (CMV) in the immunocompromised host is associated with the appearance of a T cell population with reduced CD8 and T cell receptor (TCR) expression.
abstract: Infection with human cytomegalovirus (HCMV) mostly results in a chronic subclinical infection; the immune system is unable to eliminate the virus and is apparently in equilibrium with the persistent virus. In the immunosuppressed host this equilibrium is disturbed, resulting in clinical infection. Rat cytomegalovirus (RCMV) infection in its host can be used as a model for HCMV infection. Using flow cytometry we examined the effect of acute RCMV infection on the composition of leucocyte subsets in the peripheral blood of both immunocompetent and immunosuppressed (5 Gy total body irradiation) Lewis rats. Special attention was paid to the natural killer (NK) cells and the CD8+ T cells known to be involved in the control of viral infections. Furthermore, we determined the presence of leucocyte subsets in the internal organs by immunohistochemistry. In immunocompetent rats, infection caused a small increase in NK cells and a large increase in CD8+ T cells. In contrast, infection of immunosuppressed rats caused a marked increase in NK cells and a small increase in CD8+ T cells, consisting of T cells with reduced expression of both CD8 and TCR. This phenomenon is characteristic of anergic CD8+ T cells, possibly explaining the ability of the virus to escape elimination by the immune system. The increase of NK cells in the peripheral blood of immunosuppressed, RCMV-infected rats could also be detected in kidney, liver, lung and pancreas, but not in salivary gland. This could explain the long persistence of infectious virus in the salivary gland.
authors.affiliation: Department of Medical Microbiology, University of Maastricht, The Netherlands.
authors.forename: J G
authors.initials: JG
authors.lastname: van Dam
authors.forename: J G
authors.initials: JG
authors.lastname: Damoiseaux
authors.forename: H A
authors.initials: HA
authors.lastname: Van der Heijden
authors.forename: G
authors.initials: G
authors.lastname: Grauls
authors.forename: P J
authors.initials: PJ
authors.lastname: Van Breda Vriesman
authors.forename: C A
authors.initials: CA
authors.lastname: Bruggeman
mesh_terms: D000818:Animals; D016827:CD8 Antigens / Q000032:analysis*; D003586:Cytomegalovirus Infections / Q000276:immunology*; D007108:Immune Tolerance; D016867:Immunocompromised Host; D007694:Killer Cells, Natural / Q000276:immunology; D008297:Male; D051381:Rats; D011917:Rats, Inbred Lew; D011948:Receptors, Antigen, T-Cell / Q000032:analysis*; D013601:T-Lymphocytes / Q000276:immunology*
chemical_list: D016827:CD8 Antigens; D011948:Receptors, Antigen, T-Cell