A modified pGEX vector with a C-terminal histidine tag: recombinant double-tagged protein obtained in greater yield and purity.
Strugnell
S A
SA
College of Agricultural and Life Sciences, University of Wisconsin-Madison, Madison, Wisconsin 53706, USA.
Wiefling
B A
BA
Deluca
H F
HF
D000595:Amino Acid Sequence; D001483:Base Sequence; D003001:Cloning, Molecular / Q000379:methods; D004591:Electrophoresis, Polyacrylamide Gel; D004765:Enteropeptidase / Q000378:metabolism; D005822:Genetic Vectors; D005982:Glutathione Transferase / Q000235:genetics; D006639:Histidine; D008969:Molecular Sequence Data; D009838:Oligodeoxyribonucleotides / Q000737:chemistry; D010455:Peptides / Q000235:genetics; D018167:Receptors, Calcitriol / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism; D011993:Recombinant Fusion Proteins / Q000737:chemistry / Q000302:isolation & purification*; D013917:Thrombin / Q000378:metabolism
D009838:Oligodeoxyribonucleotides; D010455:Peptides; D018167:Receptors, Calcitriol; D011993:Recombinant Fusion Proteins; C033223:polyhistidine; D006639:Histidine; D005982:Glutathione Transferase; D013917:Thrombin; D004765:Enteropeptidase