title: Sulfidoleukotriene release test (CAST) in hypersensitivity to nonsteroidal anti-inflammatory drugs.
abstract: There is a great need to develop a method for making an accurate and reliable in vitro diagnosis of adverse hypersensitivity reactions to drugs. We measured the amount of sulfidoleukotriene (sLT) released from the peripheral blood leukocytes obtained from 25 patients who developed hypersensitivity reactions following the administration of nonsteroidal anti-inflammatory drugs (NSAIDs); 12 patients demonstrated reactions to Voltaren, 8 patients to Bufferin, and 5 patients to Sedes G. The stimulation index, the ratio of the amounts of sLT (pg/ml) incubated with and without drugs, was considerably higher in the patients than in the controls, which consisted of 5 nonallergic healthy subjects. The sensitivity of the CAST (cellular antigen stimulation test) was evaluated to range from 62.5 to 80%, while the specificity was 70-100%. The CAST may thus be useful as a novel in vitro test system in order to screen for possible hypersensitive reactions to NSAIDs with both reliability and safety.
authors.affiliation: Department of Dermatology, School of Medicine, Fukuoka University, Japan.
authors.forename: Y
authors.initials: Y
authors.lastname: Kubota
authors.forename: S
authors.initials: S
authors.lastname: Imayama
authors.forename: A
authors.initials: A
authors.lastname: Toshitani
authors.forename: H
authors.initials: H
authors.lastname: Miyahara
authors.forename: T
authors.initials: T
authors.lastname: Tanahashi
authors.forename: Y
authors.initials: Y
authors.lastname: Uemura
authors.forename: T
authors.initials: T
authors.lastname: Koga
authors.forename: N
authors.initials: N
authors.lastname: Sugawara
authors.forename: F
authors.initials: F
authors.lastname: Kurimoto
authors.forename: K
authors.initials: K
authors.lastname: Hata
mesh_terms: D000328:Adult; D000368:Aged; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000009:adverse effects* / Q000276:immunology; D001241:Aspirin / Q000276:immunology; D004008:Diclofenac / Q000276:immunology; D004342:Drug Hypersensitivity / Q000175:diagnosis*; D005260:Female; D006801:Humans; D007159:Immunologic Tests / Q000379:methods*; D015289:Leukotrienes / Q000032:analysis*; D008297:Male; D008875:Middle Aged; D010328:Patch Tests
chemical_list: D000894:Anti-Inflammatory Agents, Non-Steroidal; D015289:Leukotrienes; D004008:Diclofenac; D001241:Aspirin