A comparison of (+/-)epibatidine with NMDA in releasing [3H]noradrenaline and adenosine from slices of rat hippocampus and parietal cortex.
We compared (+/-)epibatidine with N-methyl-D-aspartate (NMDA) in releasing adenosine and [3H]noradrenaline from slices of rat hippocampus and parietal cortex. (+/-)Epibatidine (0.1 microM) released [3H]noradrenaline but not adenosine from hippocampal slices incubated either with or without extracellular Mg2+. In contrast, NMDA (300 microM) released much more [3H]noradrenaline and also adenosine from hippocampal slices incubated in medium lacking Mg2+. (+/-)Epibatidine released neither adenosine nor [3H]noradrenaline from slices of rat parietal cortex, in contrast to NMDA which released both substances. These findings suggest that those behavioral responses to (+/-)epibatidine that are mediated by noradrenaline may involve the hippocampus but not the parietal cortex. Moreover, it seems unlikely that any of the behavioral effects of (+/-)epibatidine are mediated by adenosine release in either the parietal cortex or the hippocampus.
White
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Department of Pharmacology, Dalhousie University, Halifax, Nova Scotia, Canada. tom.white@dal.ca
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D000241:Adenosine / Q000378:metabolism*; D000818:Animals; D019086:Bridged Bicyclo Compounds, Heterocyclic / Q000494:pharmacology*; D006624:Hippocampus / Q000187:drug effects* / Q000378:metabolism; D066298:In Vitro Techniques; D008297:Male; D016202:N-Methylaspartate / Q000494:pharmacology*; D009638:Norepinephrine / Q000378:metabolism*; D010296:Parietal Lobe / Q000187:drug effects* / Q000378:metabolism; D011725:Pyridines / Q000494:pharmacology*; D051381:Rats; D017208:Rats, Wistar; D014316:Tritium
D019086:Bridged Bicyclo Compounds, Heterocyclic; D011725:Pyridines; D014316:Tritium; D016202:N-Methylaspartate; D000241:Adenosine; C082748:epibatidine; D009638:Norepinephrine