Simple and rapid determination of the acetaldehyde dehydrogenase (ALDH2) genotypes by nonradioactive single-strand conformation polymorphism analysis.
The genotyping of mitochondrial acetaldehyde dehydrogenase (ALDH2) is very important in alcohol studies. We describe an ALDH2 genotyping method based on nonradioactive single-strand conformation polymorphism (SSCP) analysis on mini-gels following amplification with a mutated primer set. The three ALDH2 genotypes were clearly and unambiguously distinguished. This method was applied to the ALDH2 genotyping of 129 unrelated Japanese. The allele frequency of ALDH2*2 was calculated to be 0.271, which was consistent with the previous data. The method proved to be simple, rapid and reliable, and dispensed with isotopic reagent and expensive restriction enzymes and equipment. The SSCP method described here is valuable in routine ALDH2 genotyping.
Yuasa
I
I
Department of Legal Medicine, Tottori University School of Medicine, Yonago, Japan. yuasai@grape.med.tottoriu.ac.jp
Umetsu
K
K
Nakagawa
M
M
Ikebuchi
J
J
Inoue
T
T
Irizawa
Y
Y
D000445:Aldehyde Oxidoreductases / Q000235:genetics*; D000483:Alleles; D004591:Electrophoresis, Polyacrylamide Gel; D005787:Gene Frequency; D005838:Genotype; D006801:Humans; D007527:Isoenzymes / Q000235:genetics*; D008930:Mitochondria, Liver / Q000201:enzymology*; D016133:Polymerase Chain Reaction; D018807:Polymorphism, Single-Stranded Conformational
D007527:Isoenzymes; D000445:Aldehyde Oxidoreductases; C018699:aldehyde dehydrogenase (NAD(P)+)