Comparison of the bacterial HelA protein to the F508 region of the cystic fibrosis transmembrane regulator.
The HelA protein of Rhodobacter capsulatus is the ATP-binding-cassette subunit of an exporter complex required for cytochrome c biogenesis. By primary sequence comparisons the F88 residue of HelA is similar to the F508 residue of the cystic fibrosis transmembrane regulator (CFTR) protein. Previous studies have established that CFTR F508delta or F508R proteins are defective but F508C is functional. Our results demonstrate that the HelA F88 mutants functionally mimic the phenotypes of known CFTR F508 mutants. The phenotypes of additional HelA mutants and the in vivo steady-state levels of these proteins are also reported.
Goldman
B S
BS
Department of Biology, Washington University, St. Louis, Missouri 63130-4899, USA.
Sherman
D A
DA
Kranz
R G
RG
D018528:ATP-Binding Cassette Transporters / Q000235:genetics*; D000595:Amino Acid Sequence; D001426:Bacterial Proteins; D019005:Cystic Fibrosis Transmembrane Conductance Regulator / Q000235:genetics*; D018716:Molecular Mimicry; D008969:Molecular Sequence Data; D009154:Mutation; D010641:Phenotype; D016354:Rhodobacter capsulatus / Q000235:genetics*; D017386:Sequence Homology, Amino Acid
D018528:ATP-Binding Cassette Transporters; D001426:Bacterial Proteins; C109582:HelA protein, bacteria; D019005:Cystic Fibrosis Transmembrane Conductance Regulator