The use of exfoliative cell samples to map clonal genetic alterations in the oral epithelium of high-risk patients.
Although it is widely accepted that clonal genetic alterations are an essential component of tumor progression, little is known of the distribution of such changes in high-risk lesions or how such clones are altered over time. We explored the feasibility of using exfoliative cells collected by scraping the mucosal surface to detect allelic loss in oral lesions of 22 patients (14 squamous cell carcinomas, 2 carcinomas in situ, and 6 dysplasias). The data show that the patterns of allelic loss observed in these samples closely represent those observed in biopsies of the same region. Furthermore, early indications are that this approach can be used to detect recurrent outgrowth of clones of altered cells in patients after therapy.
Rosin
M P
MP
British Columbia Cancer Research Centre, Vancouver, Canada.
Epstein
J B
JB
Berean
K
K
Durham
S
S
Hay
J
J
Cheng
X
X
Zeng
T
T
Huang
Y
Y
Zhang
L
L
D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000483:Alleles; D001706:Biopsy; D002278:Carcinoma in Situ / Q000235:genetics / Q000473:pathology; D002294:Carcinoma, Squamous Cell / Q000235:genetics* / Q000473:pathology; D002872:Chromosome Deletion; D002874:Chromosome Mapping; D002877:Chromosomes, Human; D005260:Female; D005887:Gingival Neoplasms / Q000235:genetics / Q000473:pathology; D006801:Humans; D008297:Male; D008875:Middle Aged; D009061:Mouth Mucosa / Q000473:pathology*; D009062:Mouth Neoplasms / Q000235:genetics* / Q000473:pathology; D011230:Precancerous Conditions / Q000235:genetics* / Q000473:pathology; D012307:Risk Factors; D013048:Specimen Handling; D014062:Tongue Neoplasms / Q000235:genetics / Q000473:pathology