title: Downregulation of MAP kinase activity signalled by HIV-1-gp120 coat protein in granular neurons and glial cells from rat cerebellum.
abstract: We have studied the effect of gp120 coat protein from HIV-1 on tyrosine phosphorylation processes in primary cultures of granular neurons or glial cells from the cerebellum of neonatal rats. The extracellular application of recombinant gp120 (200 pM) was able to reduce the phosphotyrosine content and the immunoreactivity for active form-specific antibodies of MAP kinase. Whereas in neurons MAP kinase appeared to be the only protein whose phosphotyrosine content was decreased, in glial cultures the inhibitory effect of gp120 on tyrosine phosphorylation processes appeared to be more widespread. In neuronal cultures, the effect of the viral protein was prevented by the concomitant treatment with depolarizing agents.
authors.affiliation: DIBIT San Raffaele Scientific Institute, B. Ceccarelli, Milano, Italy.
authors.forename: C
authors.initials: C
authors.lastname: Leoni
authors.forename: A
authors.initials: A
authors.lastname: Menegon
authors.forename: F
authors.initials: F
authors.lastname: Benfenati
authors.forename: F
authors.initials: F
authors.lastname: Valtorta
mesh_terms: D000818:Animals; D000906:Antibodies / Q000276:immunology; D015153:Blotting, Western; D017871:Calcium-Calmodulin-Dependent Protein Kinases / Q000276:immunology / Q000378:metabolism*; D002478:Cells, Cultured; D002531:Cerebellum / Q000201:enzymology*; D015536:Down-Regulation; D015699:HIV Envelope Protein gp120 / Q000235:genetics / Q000494:pharmacology*; D009457:Neuroglia / Q000201:enzymology*; D009474:Neurons / Q000201:enzymology*; D010766:Phosphorylation; D019000:Phosphotyrosine / Q000276:immunology / Q000378:metabolism; D011188:Potassium / Q000494:pharmacology; D051381:Rats; D017207:Rats, Sprague-Dawley; D011994:Recombinant Proteins / Q000494:pharmacology; D015398:Signal Transduction; D014638:Vanadates / Q000494:pharmacology
chemical_list: D000906:Antibodies; D015699:HIV Envelope Protein gp120; D011994:Recombinant Proteins; D019000:Phosphotyrosine; D014638:Vanadates; D017871:Calcium-Calmodulin-Dependent Protein Kinases; D011188:Potassium