Expressed sequence tags of medaka (Oryzias latipes) liver mRNA.
A medaka liver cDNA library was constructed in lambda ZAPII. The number of clones in this library is approximately 5 x 10(6). Three hundred sixty-one clones were randomly selected and, of these, 33 clones with over 1 kb were sequenced. These sequences were compared with GenBank and the dbEST (Re.96.0). Twenty-five clones of these 33 clones encoded 18 different genes and 2 different expressed sequence tags (ESTs). Sequences of 10 of the 18 clones had not previously been reported in fish. The codon usage of medaka genes is similar to that of Xenopus, mouse, and human genes, but not similar to that of yeast and Escherichia coli genes.
Hirono
I
I
Department of Aquatic Biosciences, Tokyo University of Fisheries, Japan. hirono@tokyo-u-fish.ac.jp
Aoki
T
T
D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D003062:Codon / Q000235:genetics; D018076:DNA, Complementary; D004926:Escherichia coli; D015723:Gene Library; D005821:Genetic Techniques; D006801:Humans; D008099:Liver / Q000378:metabolism*; D051379:Mice; D009990:Oryzias / Q000235:genetics*; D012333:RNA, Messenger / Q000235:genetics* / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000235:genetics; D012689:Sequence Homology, Nucleic Acid
D003062:Codon; D018076:DNA, Complementary; D012333:RNA, Messenger