The human alpha3b is a 'full-sized' laminin chain variant with a more widespread tissue expression than the truncated alpha3a.
We report the molecular cloning of the human laminin alpha3b chain variant and its mRNA expression pattern in adult human tissues when compared to the alpha3a variant. The mRNA encoding for the alpha3b variant is about 11 kb and the predicted translation product carries the complete set of domains typical for a 'full-sized' laminin alpha chain. Apart from the similar domain structure of alpha3b also the sequence of alpha3 resulted more closely related to the alpha5 than to the alpha4 chain. Quantitative analysis of the RNA expression in a broad panel of adult human tissues indicated that the alpha3b variant is more widely distributed than the alpha3a shorter variant.
Doliana
R
R
Divisione di Oncologia Sperimentale 2, CRO, Aviano, Italy.
Bellina
I
I
Bucciotti
F
F
Mongiat
M
M
Perris
R
R
Colombatti
A
A
D000328:Adult; D000595:Amino Acid Sequence; D000818:Animals; D002461:Cell Line, Transformed; D003001:Cloning, Molecular; D014644:Genetic Variation; D006801:Humans; D007797:Laminin / Q000096:biosynthesis / Q000737:chemistry* / Q000235:genetics*; D051379:Mice; D008969:Molecular Sequence Data; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D014018:Tissue Distribution
D007797:Laminin; D012333:RNA, Messenger; C095567:laminin alpha 3