title: Identification of nicotinic acetylcholine receptors on lymphocytes in the periphery as well as thymus in mice.
abstract: The existence of nicotinic acetylcholine receptors (nAChR) on lymphocytes remains controversial. We attempted to show the existence of nAChR on murine lymphocytes. The intraperitoneal injection of nicotine induced the lymphocytosis in the spleen on day 3. Although freshly isolated lymphocytes bound small quantities of fluorescein isothiocyanate (FITC)-conjugated alpha-bungarotoxin (alpha BuTx), they began to bind alpha BuTx after incubation in medium. In contrast to granulocytes, various lymphocyte subsets obtained from various lymphoid organs were found to bind alpha BuTx. Affinity purification of alpha BuTx-binding protein revealed that lymphocytes expressed the same nAChR molecules as those of muscle. Reverse transcriptase-polymerase chain reaction (RT-PCR) analysis showed that lymphocytes expressed the alpha-subunit mRNA of nAChR. These results suggest that lymphocytes carry nAChR on the surface and are stimulated directly via their nAChR by parasympathetic nerve stimuli.
authors.affiliation: Department of Immunology, Niigata University School of Medicine, Japan.
authors.forename: S
authors.initials: S
authors.lastname: Toyabe
authors.forename: T
authors.initials: T
authors.lastname: Iiai
authors.forename: M
authors.initials: M
authors.lastname: Fukuda
authors.forename: T
authors.initials: T
authors.lastname: Kawamura
authors.forename: S
authors.initials: S
authors.lastname: Suzuki
authors.forename: M
authors.initials: M
authors.lastname: Uchiyama
authors.forename: T
authors.initials: T
authors.lastname: Abo
mesh_terms: D000818:Animals; D002038:Bungarotoxins / Q000378:metabolism; D018929:Cell Culture Techniques; D004591:Electrophoresis, Polyacrylamide Gel; D007962:Leukocytes / Q000378:metabolism; D016131:Lymphocyte Subsets / Q000276:immunology / Q000378:metabolism*; D008218:Lymphocytosis / Q000139:chemically induced; D051379:Mice; D008809:Mice, Inbred C3H; D009538:Nicotine / Q000633:toxicity; D011978:Receptors, Nicotinic / Q000032:analysis* / Q000302:isolation & purification; D013154:Spleen / Q000276:immunology; D013950:Thymus Gland / Q000276:immunology*
chemical_list: D002038:Bungarotoxins; D011978:Receptors, Nicotinic; D009538:Nicotine