Direct detection and isolation of plasmid-bearing virulent serotypes of Yersinia enterocolitica from various foods.
A procedure was developed for direct detection, isolation, and maintenance of plasmid-bearing virulent serotypes of Yersinia enterocolitica from different food sources. Plasmid-bearing virulent strains of Y. enterocolitica representing five serotypes were simultaneously detected and isolated from enriched swab samples of artificially contaminated pork chops, ground pork, cheese, and zucchini, using Congo red binding and low-calcium-response tests. The method was also effective in isolating plasmid-bearing virulent strains of Y. enterocolitica from naturally contaminated porcine tongues. Virulence of the strains isolated from these foods was confirmed by PCR, the expression of plasmid-associated phenotypes, and mouse pathogenicity.
Bhaduri
S
S
Microbial Food Safety Research Unit, U.S. Department of Agriculture, Wyndmoor, Pennsylvania 19038, USA. sbhaduri@arserrc.gov
Cottrell
B
B
D000818:Animals; D002611:Cheese / Q000382:microbiology; D005516:Food Microbiology; D006801:Humans; D051379:Mice; D010957:Plasmids / Q000235:genetics* / Q000302:isolation & purification; D016133:Polymerase Chain Reaction; D012703:Serotyping; D013552:Swine / Q000382:microbiology; D014675:Vegetables / Q000382:microbiology; D014774:Virulence / Q000235:genetics; D015008:Yersinia enterocolitica / Q000145:classification / Q000235:genetics* / Q000472:pathogenicity*