title: Expression of glycoprotein gp43 in stage-specific forms and during dimorphic differentiation of Paracoccidioides brasiliensis.
abstract: Expression of the 43 kDa glycoprotein (gp43) was analysed in several Paracoccidioides brasiliensis isolates. Using one- and two-dimensional analysis of crude cellular extracts, it was shown that protein expression in yeast and mycelium was dependent on the isolate analysed. In two strains, in both yeast and mycelium cells. gp43 was present, whereas expression was restricted to the yeast phase of two other strains. The clinical implications of this phase-specific gp43 expression are uncertain.
authors.affiliation: Departamento de Bioquímica/Biofísica, ICB, Universidade Federal de Goiás, Goiânia, Brazil.
authors.forename: R
authors.initials: R
authors.lastname: Mattar-Filho
authors.forename: M O
authors.initials: MO
authors.lastname: Azevedo
authors.forename: M
authors.initials: M
authors.lastname: Pereira
authors.forename: R S
authors.initials: RS
authors.lastname: Jesuino
authors.forename: S M
authors.initials: SM
authors.lastname: Salem-Izacc
authors.forename: W A
authors.initials: WA
authors.lastname: Brito
authors.forename: J L
authors.initials: JL
authors.lastname: Gesztesi
authors.forename: R B
authors.initials: RB
authors.lastname: Soares
authors.forename: M S
authors.initials: MS
authors.lastname: Felipe
authors.forename: C M
authors.initials: CM
authors.lastname: Soares
mesh_terms: D000946:Antigens, Fungal / Q000096:biosynthesis*; D015153:Blotting, Western; D015180:Electrophoresis, Gel, Two-Dimensional; D004591:Electrophoresis, Polyacrylamide Gel; D005656:Fungal Proteins; D006023:Glycoproteins / Q000096:biosynthesis* / Q000302:isolation & purification; D006801:Humans; D008970:Molecular Weight; D009844:Oligosaccharides / Q000096:biosynthesis* / Q000302:isolation & purification; D010228:Paracoccidioides / Q000254:growth & development / Q000302:isolation & purification / Q000502:physiology*; D010229:Paracoccidioidomycosis / Q000382:microbiology
chemical_list: C103180:43 kDa protein, Paracoccidioides; D000946:Antigens, Fungal; D005656:Fungal Proteins; D006023:Glycoproteins; D009844:Oligosaccharides