Evidence for CYP2D6 expression in human lung.
The cytochrome P450 CYP2D6 gene (CYP2D6) expression was examined in samples from human bronchial mucosa and lung parenchyma using reverse transcription-polymerase chain reaction (RT-PCR) and immunochemistry. Except specimen from a patient previously genotyped as homozygous for a complete deletion of the gene, all tissue samples were positive. When compared to that in the liver, the mean level of CYP2D6 mRNA was 3-fold lower in bronchial mucosa and 6-fold lower in lung parenchyma. To our knowledge, these data demonstrate for the first time the presence of CYP2D6 protein in human lung. They also indicate that the gene is nonuniformly distributed within this organ. The possibility that CYP2D6 has a role in lung carcinogenesis by locally activating inhaled chemicals should therefore be considered.
Guidice
J M
JM
Laboratoire de Biochimie et Biologie Moléculaire, Clinique de Pneumophtisiologie, Hôpital Calmette, Centre Hospitalier Régional et Universitaire de Lille, Boulevard J. Leclercq, Lille, 59037, France.
Marez
D
D
Sabbagh
N
N
Legrand-Andreoletti
M
M
Spire
C
C
Alcaïde
E
E
Lafitte
J J
JJ
Broly
F
F
D001980:Bronchi; D003001:Cloning, Molecular; D019389:Cytochrome P-450 CYP2D6 / Q000096:biosynthesis* / Q000235:genetics*; D017931:DNA Primers; D004926:Escherichia coli; D017353:Gene Deletion; D005838:Genotype; D006801:Humans; D008168:Lung / Q000201:enzymology*; D008175:Lung Neoplasms / Q000201:enzymology*; D008861:Microsomes / Q000201:enzymology*; D008862:Microsomes, Liver / Q000201:enzymology; D009092:Mucous Membrane / Q000201:enzymology; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000096:biosynthesis; D011994:Recombinant Proteins / Q000096:biosynthesis; D014132:Trachea; D014158:Transcription, Genetic
D017931:DNA Primers; D012333:RNA, Messenger; D011994:Recombinant Proteins; D019389:Cytochrome P-450 CYP2D6