Fibroblasts obtained from human nasal, laryngeal and tracheal mucosa produce the chemokine RANTES.
RANTES is a chemokine that was already found in tissues obtained from nasal polyps of patients suffering from chronic polypous sinusitis and in lung biopsies of patients suffering from bronchial asthma. Nasal fibroblasts could be shown to be a cellular origin of RANTES. The aim of this study was to investigate whether human nasal, laryngeal and tracheal mucosa fibroblasts are differentiated in production of RANTES. Fibroblasts obtained from healthy human nasal, laryngeal and tracheal mucosa, were cultured. Secretion of RANTES-protein in supernatants was investigated after stimulation with 50 ng/ml tumor necrosis factor-alpha (TNF-alpha), interleukin-1-beta (IL-1-beta), lipopolysaccharide (LPS), phorbolmyrisate acetate (PMA), interferon-gamma (IFN-gamma) and serum-free medium (SFM) for 24 hours. Cultivated nasal, laryngeal and tracheal fibroblasts secreted RANTES-protein upon TNF-alpha, IL-1-beta and IFN-gamma stimulation. The amounts of RANTES-protein production ranged from 10 ng/ml (PMA) to 198 ng/ml (TNF-alpha). Secretion of significant amounts of RANTES-protein were detected in the supernatants from either nasal, laryngeal or tracheal fibroblasts. There was no significant difference between the differential fibroblasts. We conclude that nasal, laryngeal and tracheal fibroblasts could be a cellular source of RANTES in nasal and bronchial mucosa or in secrets of patients suffering from diseases where eosinophilic tissue infiltration represents a characteristic histopathological feature. Results suggest that additional local factors are needed to develop asthma bronchiale and chronic polypous sinusitis.
Maune
S
S
Dept. of Otorhinolaryngology, Head and Neck Surgery, University of Kiel, Germany.
Werner
J A
JA
Sticherling
M
M
Schröder
J M
JM
D018946:Chemokine CCL5 / Q000378:metabolism*; D004797:Enzyme-Linked Immunosorbent Assay; D005347:Fibroblasts / Q000378:metabolism; D006801:Humans; D007830:Larynx / Q000378:metabolism*; D009297:Nasal Mucosa / Q000378:metabolism*; D014132:Trachea / Q000378:metabolism*
D018946:Chemokine CCL5