Role of the sulfhydryl redox state and disulfide bonds in processing and assembly of 11S seed globulins.
Seed legumins contain two conserved disulfide bonds: an interchain bond (IE) connecting the acidic and basic chains and an intrachain bond (IA) internal to the acidic chain. Mutant subunits were constructed in which these disulfide bonds were disrupted. Oxidized glutathione stimulated the rate of assembly of trimers with unmodified prolegumin subunits. Stimulation was not detected during assembly of IE mutant subunits and was diminished for the IA mutant. Hexamer assembly with trimers of mature unmodified subunits required oxidizing conditions. Trimers composed of mature IE mutants did not form hexamers. Both mutant and non-mutant subunits accumulated in hexamers when the cDNAs were expressed in tobacco. Hexamer assembly in seeds probably involved trimers with a mixture of mutant and non-mutant subunits. Similarly, mixed trimers that were a mixture of mutant and non-mutant subunits assembled into hexamers in vitro. The results demonstrate the importance of disulfide bonds during the assembly of 11S globulins.
Jung
R
R
United States Department of Agriculture, Agricultural Research Service, West Lafayette, Indiana.
Nam
Y W
YW
Saalbach
I
I
Müntz
K
K
Nielsen
N C
NC
D001704:Biopolymers; D003545:Cysteine / Q000235:genetics; D004220:Disulfides / Q000737:chemistry / Q000378:metabolism*; D005916:Globulins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D005978:Glutathione / Q000737:chemistry / Q000378:metabolism*; D009154:Mutation; D010084:Oxidation-Reduction; D010940:Plant Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D010947:Plants, Toxic; D011499:Protein Processing, Post-Translational; D014026:Nicotiana / Q000378:metabolism; D014170:Transformation, Genetic; D014617:Vacuoles / Q000378:metabolism; D000097793:Legumins
D001704:Biopolymers; D003545:Cysteine; D004220:Disulfides; D005916:Globulins; D005978:Glutathione; D010940:Plant Proteins; D000097793:Legumins