Cloning and expression of the cDNA for canine interleukin-12.
We cloned the canine interleukin-12 (IL-12) subunit cDNA. Canine IL-12 exhibited sequence homology to the known sequences of human, mouse, and bovine genes at nucleotide and amino acid levels. Cotransfection of the p35 and p40 subunits of canine IL-12 cDNA clones into COS-1 cells resulted in the secretion of IL-12, which supported proliferation of the stimulated canine lymphocytes, promoted induction of canine interferon-gamma (IFN-gamma) from canine lymphocytes, and showed antitumor effect in vitro. The cloned canine IL-12 will be useful for canine therapeutic applications.
Okano
F
F
Toray Industries, Inc., Chemicals Research Laboratories, Nagoya, Japan.
Satoh
M
M
Yamada
K
K
D000595:Amino Acid Sequence; D000818:Animals; D000970:Antineoplastic Agents / Q000627:therapeutic use*; D001483:Base Sequence; D002417:Cattle; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000302:isolation & purification*; D004285:Dogs; D006801:Humans; D018664:Interleukin-12 / Q000235:genetics* / Q000627:therapeutic use; D051379:Mice; D008969:Molecular Sequence Data; D011994:Recombinant Proteins / Q000627:therapeutic use; D017386:Sequence Homology, Amino Acid
D000970:Antineoplastic Agents; D018076:DNA, Complementary; D011994:Recombinant Proteins; D018664:Interleukin-12