Using polymerase chain reaction to human papillomavirus in oral and pharyngolaryngeal carcinomas.
OBJECTIVE
Increasingly, evidence has shown that human papillomavirus (HPV) plays a role in the induction of certain carcinomas. The presence of HPV sequences in 56 previously untreated oral and pharyngolaryngeal carcinomas was examined by the polymerase chain reaction (PCR).


METHODS
After DNA extraction, samples underwent 40 replication cycles with specific oligonucleotide primers corresponding to sequences from the E6 open-reading frame of HPV-6b, HPV-16, and HPV-18. To determine the E6 genomic integration, positive samples were processed with specific primers for the corresponding HPV L1 genes. Genomic HPV DNA clones into PBR 322 was used as positive control.


RESULTS
HPV E6 DNA of the 6b and 16 types was detected in 14 patients (25%). The L1 gene was not present.


CONCLUSIONS
Detected HPV E6 DNA might be integrated into the cell genome in the positive cases as indicated by the absence of the L1 gene-coding for the viral capside. Histological and survival rates, were unrelated to the presence of HPV.
Alvarez Alvarez
I
I
Department of Otorhinology, Complejo Hospitalario de León, Oviedo, Spain.
Sanchez Lazo
P
P
Ramos Gonzalez
S
S
Rodrigo Tapia
J P
JP
Nuñez Batalla
F
F
Suarez Nieto
C
C
D000328:Adult; D000368:Aged; D002294:Carcinoma, Squamous Cell / Q000235:genetics* / Q000401:mortality; D004273:DNA, Neoplasm / Q000032:analysis*; D004279:DNA, Viral / Q000032:analysis*; D005260:Female; D005838:Genotype; D006801:Humans; D007822:Laryngeal Neoplasms / Q000235:genetics* / Q000401:mortality; D008297:Male; D008875:Middle Aged; D009959:Oropharyngeal Neoplasms / Q000235:genetics* / Q000401:mortality; D027383:Papillomaviridae / Q000235:genetics*; D016133:Polymerase Chain Reaction; D015996:Survival Rate
D004273:DNA, Neoplasm; D004279:DNA, Viral