title: Distinctive IS200 insertion between gyrA and rcsC genes in Salmonella typhi.
abstract: While probing the vicinity of ompC, a copy of the IS200 insertion element was found between the gyrA and rcsC genes of Salmonella typhi, the causal agent of typhoid fever. This distinctive feature was conserved throughout 63 S. typhi isolates of different geographical origins and was absent from 46 other Salmonella serotypes, including those most associated with human infections, as well as from 19 other enteric bacteria. Furthermore, the location of this IS200 copy corresponds to a constant band, present throughout the 14 PstI S. typhi IS200 fingerprints, encompassing several Vi phage types. Interestingly, an apparently unrelated serotype not frequently associated with human disease, Salmonella weltevreden, contained an IS200 copy at the same position, although it was accompanied by an additional segment of cryptic DNA. On the basis of these findings, PCR assays were designed for molecular typing of S. typhi, and these are potentially useful in studying the epidemiology of typhoid fever.
authors.affiliation: Departamento de Microbiología Molecular, Instituto de Biotecnología, Universidad Nacional Autónoma de México, Cuernavaca, Morelos. ecalva@ibt.unam.mx
authors.forename: E
authors.initials: E
authors.lastname: Calva
authors.forename: L G
authors.initials: LG
authors.lastname: Ordoñez
authors.forename: M
authors.initials: M
authors.lastname: Fernandez-Mora
authors.forename: F J
authors.initials: FJ
authors.lastname: Santana
authors.forename: M
authors.initials: M
authors.lastname: Bobadilla
authors.forename: J L
authors.initials: JL
authors.lastname: Puente
mesh_terms: D001426:Bacterial Proteins / Q000235:genetics; D001483:Base Sequence; D002874:Chromosome Mapping; D017124:Conserved Sequence; D016172:DNA Fingerprinting; D027081:DNA Gyrase; D017931:DNA Primers / Q000235:genetics; D004250:DNA Topoisomerases, Type II / Q000235:genetics; D004251:DNA Transposable Elements; D004269:DNA, Bacterial / Q000235:genetics / Q000302:isolation & purification; D005798:Genes, Bacterial; D016680:Genome, Bacterial; D006801:Humans; D017720:Molecular Epidemiology; D008969:Molecular Sequence Data; D009097:Multienzyme Complexes; D010749:Phosphoprotein Phosphatases; D016133:Polymerase Chain Reaction; D011494:Protein Kinases; D012485:Salmonella typhi / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification; D012703:Serotyping; D014157:Transcription Factors / Q000235:genetics; D014435:Typhoid Fever / Q000453:epidemiology / Q000382:microbiology
chemical_list: D001426:Bacterial Proteins; D017931:DNA Primers; D004251:DNA Transposable Elements; D004269:DNA, Bacterial; D009097:Multienzyme Complexes; D014157:Transcription Factors; D011494:Protein Kinases; D010749:Phosphoprotein Phosphatases; D027081:DNA Gyrase; D004250:DNA Topoisomerases, Type II