Identification of genes specifically expressed in chronic and progressive glomerulosclerosis.
To identify genes expressed predominantly in the kidney with chronic and progressive glomerulosclerosis but not in acute and transient form of glomerulonephritis, we induced progressive glomerulosclerosis in rats by applying unilateral nephrectomy prior to injection of monoclonal anti-Thy1.1 antibody (OX-7), which cause acute and transient glomerulonephritis with single injection. In rats with nephrectomy and OX-7 injection (Nx group), proteinuria increased with time and mesangial expansion accompanied with interstitial fibrosis was recognized, whereas transient proteinuria and mesangiolysis followed by mesangial hypercellularity were seen in rats with sham operation and OX-7 injection (Sham group). Four weeks after the induction of glomerulonephritis, mRNAs were isolated from kidney cortex of both groups and used for cDNA synthesis. By subtraction hybridization of cDNAs from Nx with excess amount of those from Sham, we isolated and sequenced several genes expressed specifically in the Nx group. These included genes, which contain identical sequences with serine protease inhibitors, cytokine receptors, osteopontin as well as genes with unknown function. These genes may play important roles in the process which promotes acute glomerular damage advance to chronic and progressive glomerulosclerosis.
Narita
I
I
Department of Medicine II, Niigata University Medical School, Japan. naritai@med.niigata-u.ac.jp
Nakayama
H
H
Goto
S
S
Takeda
T
T
Sakatsume
M
M
Saito
A
A
Nakagawa
Y
Y
Arakawa
M
M
D000818:Animals; D000911:Antibodies, Monoclonal; D002908:Chronic Disease; D018450:Disease Progression; D004587:Electrophoresis, Agar Gel; D005923:Glomerulosclerosis, Focal Segmental / Q000235:genetics* / Q000473:pathology / Q000652:urine; D008297:Male; D016133:Polymerase Chain Reaction; D011507:Proteinuria / Q000235:genetics / Q000473:pathology; D051381:Rats; D017207:Rats, Sprague-Dawley
D000911:Antibodies, Monoclonal