title: Follicle-stimulating hormone receptor mRNA in the mouse ovary during post-natal development in the normal mouse and in the adult hypogonadal (hpg) mouse: structure of alternate transcripts.
abstract: The structure of RNA encoding the mouse ovarian follicle-stimulating hormone (FSH) receptor was studied during post-natal development and in the adult hypogonadal (hpg) mouse which lacks circulating gonadotrophins. Using reverse transcription and the polymerase chain reaction (PCR) four major transcripts of the FSH receptor were found in the normal adult ovary. The largest transcript was the expected size from the position of the PCR primers (on exons 1 and 10) and sequencing confirmed that it was derived from FSH receptor mRNA. The three other transcripts were also derived from FSH receptor mRNA but they contained deletions corresponding to one or more complete exons. Each transcript lacked exon 2 while exons 5 and/or 6 were lacking in the smaller species. All four transcripts were present in ovaries of hpg mice showing that expression of receptor mRNA and development of alternate splicing are not gonadotrophin-dependent. During development in the mouse full-length FSH receptor transcripts were not detected in the ovary until day 5 although shorter transcripts were present at days 1 and 3. Results confirm that the FSH receptor primary transcript undergoes alternate splicing in the ovary and that the pattern of splicing changes as the ovary develops, probably as a result of follicular development.
authors.affiliation: Department of Veterinary Physiology, University of Glasgow Veterinary School, UK.
authors.forename: P J
authors.initials: PJ
authors.lastname: O'Shaughnessy
authors.forename: P
authors.initials: P
authors.lastname: Marsh
authors.forename: K
authors.initials: K
authors.lastname: Dudley
mesh_terms: D017398:Alternative Splicing; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D005260:Female; D005640:Follicle Stimulating Hormone / Q000378:metabolism*; D051379:Mice; D008817:Mice, Mutant Strains; D008969:Molecular Sequence Data; D010053:Ovary / Q000254:growth & development / Q000378:metabolism*; D012333:RNA, Messenger / Q000235:genetics*; D011962:Receptors, FSH / Q000096:biosynthesis / Q000235:genetics*
chemical_list: D012333:RNA, Messenger; D011962:Receptors, FSH; D005640:Follicle Stimulating Hormone