Campylobacter jejuni isolates from Japanese patients with Guillain-Barré syndrome.
Serologic evidence of recent Campylobacter jejuni infection was found in 92 (45%) of 205 Japanese patients with Guillain-Barré syndrome (GBS), and 49% of those 92 patients also had antibodies to GM1. Sixteen independent clinical isolates from GBS patients were serotyped: 12 belonged to Penner's heat-stable (HS) O serotype HS-19, 3 to HS-2, and 1 to HS-4. Of the patients whose C. jejuni isolates belonged to HS-19, 80% had elevated anti-GM1 antibodies. Although the correlation was significant between C. jejuni and GM1 antibody, anti-GM1 also was detected in 25% of patients without C. jejuni infection. Polymerase chain reaction-based restriction fragment length polymorphism analysis of an flaA gene showed that all HS-19 isolates, regardless of a GBS association, had an identical and distinguishable pattern, Cj-1, suggesting that HS-19:Cj-1 isolates are distinctive among C. jejuni isolates. Lectin typing showed that all GBS-associated HS-19 isolates contained terminal beta-N-acetylglucosamine residues on their cell surface, but HS-19 isolates from patients with enteritis did not.
Saida
T
T
Department of Neurology and Clinical Research Center, Utano National Hospital, and Kyoto Microbiological Institute, Japan.
Kuroki
S
S
Hao
Q
Q
Nishimura
M
M
Nukina
M
M
Obayashi
H
H
D000293:Adolescent; D000328:Adult; D000368:Aged; D000907:Antibodies, Bacterial / Q000097:blood; D001323:Autoantibodies / Q000097:blood; D015373:Bacterial Typing Techniques; D002169:Campylobacter Infections / Q000150:complications / Q000453:epidemiology / Q000382:microbiology*; D016123:Campylobacter jejuni / Q000145:classification / Q000276:immunology / Q000302:isolation & purification*; D002648:Child; D002675:Child, Preschool; D004269:DNA, Bacterial / Q000032:analysis; D005260:Female; D005408:Flagellin / Q000235:genetics; D005677:G(M1) Ganglioside / Q000276:immunology; D006017:Glycolipids / Q000276:immunology; D006801:Humans; D007564:Japan / Q000453:epidemiology; D037102:Lectins / Q000032:analysis; D008297:Male; D008875:Middle Aged; D019081:O Antigens / Q000032:analysis / Q000276:immunology; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length; D011129:Polyradiculoneuropathy / Q000453:epidemiology / Q000209:etiology / Q000382:microbiology*; D016036:Seroepidemiologic Studies
D000907:Antibodies, Bacterial; D001323:Autoantibodies; D004269:DNA, Bacterial; D006017:Glycolipids; D037102:Lectins; D019081:O Antigens; D005408:Flagellin; C067138:flaA protein, bacteria; D005677:G(M1) Ganglioside