Folding of an mRNA pseudoknot required for stop codon readthrough: effects of mono- and divalent ions on stability.
Unfolding of an mRNA pseudoknot that induces ribosome suppression of the gag gene stop codon in Moloney murine leukemia virus has been studied by UV hyperchromicity and calorimetry. The pseudoknot melts in two steps, corresponding to its two helical stems. The total enthalpy of denaturation is approximately 170 kcal/mol, approximately the value expected for the secondary structure. At low salt concentrations (<50 mM KCl) the unfolding transitions are not two-state, but they approach two-state behavior at higher salt concentrations. The structure is preferentially stabilized by smaller alkali metal ions (Li+ > Na+ > K+ > Rb+ > Cs+) and by NH4+; the same preferences are exhibited by one of the stems in the context of a hairpin. Divalent metal ions are not required to fold the pseudoknot but do stabilize it further. To examine divalent ion effects over a wide concentration range, urea was used to lower the RNA unfolding temperature and was shown not to affect characteristics of the pseudoknot unfolding in other respects. The pseudoknot binds divalent ions somewhat more tightly than a hairpin but shows only weak selectivity for different size ions. It is suggested that a region of "intermediate" divalent ion binding affinity, in between highly ligated specific sites and purely delocalized ion binding in character, is created by the pseudoknot fold but that nonspecific, delocalized ion binding contributes at least half the free energy of pseudoknot stabilization by Mg2+.
Gluick
T C
TC
Department of Chemistry, Johns Hopkins University, Baltimore, Maryland 21218, USA.
Wills
N M
NM
Gesteland
R F
RF
Draper
D E
DE
D000818:Animals; D002151:Calorimetry; D002413:Cations, Divalent / Q000378:metabolism / Q000494:pharmacology*; D002414:Cations, Monovalent / Q000378:metabolism / Q000494:pharmacology*; D018388:Codon, Terminator / Q000235:genetics; D015750:Genes, gag / Q000235:genetics; D008672:Metals, Alkali / Q000494:pharmacology; D051379:Mice; D008979:Moloney murine leukemia virus / Q000737:chemistry / Q000235:genetics*; D009690:Nucleic Acid Conformation / Q000187:drug effects*; D009691:Nucleic Acid Denaturation; D000644:Quaternary Ammonium Compounds / Q000494:pharmacology; D012333:RNA, Messenger / Q000737:chemistry* / Q000378:metabolism; D012367:RNA, Viral / Q000737:chemistry* / Q000378:metabolism; D012260:Ribonucleases / Q000378:metabolism; D017423:Sequence Analysis, RNA; D013696:Temperature; D013816:Thermodynamics; D014508:Urea / Q000494:pharmacology
D002413:Cations, Divalent; D002414:Cations, Monovalent; D018388:Codon, Terminator; D008672:Metals, Alkali; D000644:Quaternary Ammonium Compounds; D012333:RNA, Messenger; D012367:RNA, Viral; D014508:Urea; D012260:Ribonucleases