title: Endothelial-derived nitric oxide preserves anticoagulant heparan sulfate expression in cultured porcine aortic endothelial cells.
abstract: Nitric oxide (NO) has been shown to inhibit platelet adhesion and aggregation, but there are no reports on its interaction with the coagulation system. We investigated the effect of the L-arginine analogues, N-nitro-L-arginine (LNA), N(G)-nitro-L-arginine methyl ester (L-NAME), and N(G)-monomethyl-L arginine (L-NMMA), competitive inhibitors of NO production, on endothelial-surface heparan sulfate. Addition of LNA to porcine aortic endothelial cells reduced 125I-labeled antithrombin III binding to the cell surface heparan sulfate in a dose- and time-dependent fashion. Significant inhibition was observed with 1 mM LNA, and the maximal suppression (-50% of control) occurred at 10 mM LNA after 12 h. L-NAME (1 mM) and L-NMMA (1 mM) also significantly inhibited the antithrombin III binding. The iron chelator desferrioxamine significantly prevented the reduction of antithrombin III binding to LNA-treated cells. We further investigated the effect of L-NAME on intracellular oxidative stress of endothelial cells using a hydroperoxide-sensitive fluorochrome, carboxy-dichloro-dihydrofluorescein diacetate bisacetoxymethyl ester probe, and revealed that inhibition of NO synthesis by L-NAME led to a marked increase in intracellular oxidative stress. These results demonstrated that the prolonged inhibition of NO synthesis in porcine aortic endothelial cells decreases the expression of anticoagulant heparan sulfate on endothelial cells through the increase in intracellular oxidative stress, perhaps comprising another mechanism by which NO affects the coagulation system in the vasculature.
authors.affiliation: Department of Cardiology, Jichi Medical School, Minamikawachi-Machi, Tochigi, Japan.
authors.forename: M
authors.initials: M
authors.lastname: Irokawa
authors.forename: M
authors.initials: M
authors.lastname: Nishinaga
authors.forename: U
authors.initials: U
authors.lastname: Ikeda
authors.forename: Y
authors.initials: Y
authors.lastname: Shinoda
authors.forename: M
authors.initials: M
authors.lastname: Suematsu
authors.forename: N
authors.initials: N
authors.lastname: Gouda
authors.forename: Y
authors.initials: Y
authors.lastname: Ishimura
authors.forename: K
authors.initials: K
authors.lastname: Shimada
mesh_terms: D000818:Animals; D000990:Antithrombin III / Q000187:drug effects / Q000378:metabolism; D001011:Aorta / Q000166:cytology / Q000378:metabolism*; D002462:Cell Membrane / Q000378:metabolism; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D004730:Endothelium, Vascular / Q000166:cytology / Q000378:metabolism*; D005452:Fluoresceins; D006497:Heparan Sulfate / Q000096:biosynthesis* / Q000737:chemistry; D008856:Microscopy, Fluorescence; D019331:NG-Nitroarginine Methyl Ester / Q000494:pharmacology; D009569:Nitric Oxide / Q000037:antagonists & inhibitors / Q000502:physiology*; D019335:Nitroarginine / Q000494:pharmacology; D013552:Swine
chemical_list: D000990:Antithrombin III; D005452:Fluoresceins; D006497:Heparan Sulfate; D019331:NG-Nitroarginine Methyl Ester; D009569:Nitric Oxide; D019335:Nitroarginine; C053897:5(6)-carboxy-2',7'-dichlorofluorescein