title: The methylated DNA binding protein-2-H1 (MDBP-2-H1) consists of histone H1 subtypes which are truncated at the C-terminus.
abstract: The methylated DNA binding protein-2-H1 (MDBP-2-H1), present in rooster liver, is a member of the histone H1 family which inhibits transcription by binding selectively to methylated promoters. Here we have determined the primary structure of MDBP-2-H1. A comparison between histone H1 and MDBP-2-H1 was achieved by analyzing reversed phase HPLC-purified and V8-digested proteins by mass spectrometry and/or microsequencing. In rooster liver the most abundant histone H1 subtypes are H1 01 and H1 11L. Similarly, MDBP-2-H1 contains the same subtypes of histone H1. The histone H1 subtype H1 01 in MDBP-2-H1 has 150 amino acids, whereas the full-size histone H1 01 is 218 amino acids. The difference in mass between the two proteins is explained by C-terminal truncation of histone H1 01.
authors.affiliation: Friedrich-Miescher Institut, PO Box 2543, CH-4002 Basel, Switzerland.
authors.forename: S
authors.initials: S
authors.lastname: Schwarz
authors.forename: D
authors.initials: D
authors.lastname: Hess
authors.forename: J P
authors.initials: JP
authors.lastname: Jost
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D002645:Chickens; D002851:Chromatography, High Pressure Liquid; D004268:DNA-Binding Proteins / Q000737:chemistry*; D006657:Histones / Q000737:chemistry* / Q000145:classification / Q000302:isolation & purification; D008297:Male; D013058:Mass Spectrometry; D008745:Methylation; D008969:Molecular Sequence Data; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid
chemical_list: D004268:DNA-Binding Proteins; D006657:Histones