Enhanced expression in seminoma of human zinc finger genes located on chromosome 19.
Six Krüppel-type zinc finger (ZF) genes were cloned from a seminoma cDNA library. One, ZFS-1, showed high sequence homology to the ZNF91 KRAB (Krüppel-associated box) ZF gene family and also the same chromosomal assignment. Interestingly, Northern blot analyses using ZFS-1 and ZNF91 revealed that multiple ZF genes on chromosome 19 were predominantly expressed in seminomas. In addition, the testis and the seminoma showed specific expression of 2.3 kb transcript. Our results suggest that ZF genes on chromosome 19 may be implicated in the development and/or growth of seminomas.
Ogawa
T
T
Department of Urology, Yokohama City University, School of Medicine, Japan.
Poncelet
D A
DA
Kinoshita
Y
Y
Noce
T
T
Takeda
M
M
Kawamoto
K
K
Udagawa
K
K
Lecocq
P J
PJ
Marine
J C
JC
Martial
J A
JA
Hosaka
M
M
D000595:Amino Acid Sequence; D001483:Base Sequence; D015152:Blotting, Northern; D002874:Chromosome Mapping; D002888:Chromosomes, Human, Pair 19; D018076:DNA, Complementary / Q000737:chemistry / Q000302:isolation & purification; D006801:Humans; D008969:Molecular Sequence Data; D018239:Seminoma / Q000235:genetics*; D016335:Zinc Fingers / Q000235:genetics*
D018076:DNA, Complementary