title: Radioimmunoscintigraphy with a novel monoclonal antiprostate antibody (E4): an experimental study in nude mice.
abstract: BACKGROUND
  Prostate cancer is one of the leading causes of death among men, despite achievements in diagnosis and therapy. Radioimmunolocalization and radioimmunotherapy of malignant tumors have demonstrated increasing potential and may become useful tools in the management of prostate cancer.
  
  
  METHODS
  Nude mice were inoculated subcutaneously with cells from the poorly differentiated human prostate cancer cell line DU-145. The intact monoclonal antibody (MoAb) E4 and an intact anticytokeratin-8 MoAb, TS1, used for comparison were labeled with 125I and injected intraperitoneally (i.p.) in the mice. Repetitive quantitative scintigraphic recordings were performed during 1 month. The mice were killed at Day 29 after injection of the radiolabeled MoAb. The tumors and the organs were dissected and weighed. The remaining activity was measured in a gamma well counter. One part of the tumor was immediately fixed in Bouin's solution for autoradiography and the other in formaldehyde for microscopy.
  
  
  RESULTS
  The study demonstrated significant radioimmunolocalization of the MoAb E4 into the DU-145 prostate tumor tissue in the animal model, with an average radiation dose of 0.08 Gy/MBq in the tumor. TS1 localized preferentially in necrotic parts of the tumor, yielding a tumor dose of 0.02 Gy/MBq.
  
  
  CONCLUSIONS
  The MoAb E4 is a promising radiotracer for prostate cancer and may be used in radioimmunotherapy. As in earlier studies, TS1 shows significant radioimmunolocalization into necrotic tumor tissue, which also exists in prostate cancer.
authors.affiliation: Department of Diagnostic Radiology, University Hospital of Northern Sweden, Umeå.
authors.forename: A
authors.initials: A
authors.lastname: Rydh
authors.forename: K
authors.initials: K
authors.lastname: Riklund Ahlström
authors.forename: A
authors.initials: A
authors.lastname: Widmark
authors.forename: L
authors.initials: L
authors.lastname: Johansson
authors.forename: S
authors.initials: S
authors.lastname: Nilsson
authors.forename: A
authors.initials: A
authors.lastname: Bergh
authors.forename: J E
authors.initials: JE
authors.lastname: Damber
authors.forename: T
authors.initials: T
authors.lastname: Stigbrand
authors.forename: S O
authors.initials: SO
authors.lastname: Hietala
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal; D001345:Autoradiography; D006801:Humans; D007457:Iodine Radioisotopes; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D011467:Prostate / Q000276:immunology*; D011471:Prostatic Neoplasms / Q000000981:diagnostic imaging*; D016719:Radioimmunodetection
chemical_list: D000911:Antibodies, Monoclonal; D007457:Iodine Radioisotopes