Interleukin-2 production by cultured human granulosa cells.
OBJECTIVE
To determine whether cultured human granulosa cells (GC) produce Interleukin (IL)-2 and whether this GC IL-2 production may be regulated by human chorionic gonadotropin (hCG).


METHODS
Human GC derived from preovulatory follicles during in vitro fertilization (IVF) cycles were cultured in the absence or presence of hCG. Interleukin-2 was measured in tissue-culture medium by an enzyme-linked immunosorbent assay (ELISA) system.


RESULTS
Cultured GCs were demonstrated to produce IL-2 in vitro. Moreover, a positive, but not statistically significant, dose-response effect was demonstrated between culture supernatant IL-2 levels and hCG concentration.


CONCLUSIONS
Our data indicate that human GC produce IL-2 and that this production might be regulated by hCG. These results lend credence to the theory that IL-2 might be involved in the events leading to ovarian hyperstimulation syndrome (OHSS).
Orvieto
R
R
Department of Obstetrics and Gynecology, Rabin Medical Center-Campus Golda (Hasharon Hospital), Petah Tikva, Israel.
Genazzani
A R
AR
Petraglia
F
F
Schwartz
A
A
Shelef
M
M
Ben-Rafael
Z
Z
D002478:Cells, Cultured; D006063:Chorionic Gonadotropin / Q000008:administration & dosage / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D004797:Enzyme-Linked Immunosorbent Assay; D004958:Estradiol / Q000378:metabolism; D005260:Female; D005307:Fertilization in Vitro; D005500:Follow-Up Studies; D006107:Granulosa Cells / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D006801:Humans; D007376:Interleukin-2 / Q000096:biosynthesis*; D016471:Ovarian Hyperstimulation Syndrome / Q000209:etiology / Q000378:metabolism / Q000517:prevention & control; D011374:Progesterone / Q000378:metabolism
D006063:Chorionic Gonadotropin; D007376:Interleukin-2; D011374:Progesterone; D004958:Estradiol