Augmented antibody-mediated footpad responses in mice treated with an anti-IL-4 monoclonal antibody.
The effect of the anti-IL-4 monoclonal antibody 11B11 mAb on antigen-induced footpad responses in mice in which Arthus reaction is involved was investigated. Antigen-induced footpad responses were induced by immunization with hen egg lysozyme (HEL) and by challenge injection of HEL into the footpads on day 20 after the immunization. Five hours after the challenge injection, footpad swelling was measured. 11B11 mAb was daily administered i.p. over a period of 10 days, commencing on the day of immunization with HEL. The results showed that the treatment with 11B11 mAb significantly augmented the footpad swelling. There was an increase in the production of anti-HEL IgG2a antibodies in 11B11 mAb-treated mice, while a decrease in anti-HEL IgG1 and IgG antibody production was observed in the animals. The secretion of IL-4 from lymphoid cells of 11B11 mAb-treated mice was markedly reduced. In contrast, increased IFN-gamma production was observed in these animals. Thus, treatment with anti-IL-4 antibodies appears to be effective in augmenting antibody-mediated in vivo responses in which antigen-specific IgG2a antibodies and IFN-gamma produced following the antibody treatment play a role.
Yoshino
S
S
Department of Microbiology, Saga Medical School, Japan. yoshins@post.saga-med.ac.jp
D000818:Animals; D000911:Antibodies, Monoclonal / Q000494:pharmacology*; D000941:Antigens / Q000276:immunology / Q000494:pharmacology; D001183:Arthus Reaction / Q000276:immunology*; D004487:Edema / Q000188:drug therapy* / Q000276:immunology; D004527:Egg Proteins / Q000276:immunology; D006614:Hindlimb; D007074:Immunoglobulin G / Q000096:biosynthesis* / Q000276:immunology; D007371:Interferon-gamma / Q000096:biosynthesis / Q000378:metabolism; D015847:Interleukin-4 / Q000276:immunology* / Q000378:metabolism; D008198:Lymph Nodes / Q000166:cytology / Q000378:metabolism; D008297:Male; D051379:Mice; D008811:Mice, Inbred DBA; D009113:Muramidase / Q000276:immunology
D000911:Antibodies, Monoclonal; D000941:Antigens; D004527:Egg Proteins; D007074:Immunoglobulin G; D015847:Interleukin-4; D007371:Interferon-gamma; D009113:Muramidase