title: Comparison of alveolar bone loss, alveolar bone density and second metacarpal bone density, salivary and gingival crevicular fluid interleukin-6 concentrations in healthy premenopausal and postmenopausal women on estrogen therapy.
abstract: BACKGROUND
  Osteoporosis is an age-related metabolic bone disease characterized by decreased mass and increased susceptibility to fracture. The literature suggests a relationship between oral bone loss and skeletal osteoporosis; however, most studies have produced conflicting results. The purpose of this study was to determine if a relationship exists among alveolar bone loss, alveolar bone density, second metacarpal density, salivary and gingival crevicular fluid interleukin 6 (IL-6), and IL-8 concentrations in premenopausal and postmenopausal healthy women receiving estrogen therapy.
  
  
  METHODS
  Twenty-eight healthy women (aged 23-78) were evaluated for this study. A vertical bitewing and hand radiographs were taken, and the subjects were evaluated for the presence of active periodontitis. The bitewing and hand radiographs were digitized, and measurements were made from the cemento-enamel junction to the alveolar crest from both arches. Bone density was evaluated in the maxillary and mandibular alveolar process and at the mid-shaft of the second metacarpal. Percent cortical area and the moment of inertia measurements were also determined. Stimulated whole saliva was collected for a 5-min period using a cube of paraffin as a stimulant and was analyzed for total protein by a colorimetric reaction and IL-6 and IL-8 by ELISA.
  
  
  RESULTS
  The results of the study showed that postmenopausal women on estrogen therapy had more alveolar bone loss, more missing teeth, and reduced alveolar and second metacarpal bone density than premenopausal women. In addition, postmenopausal women on estrogen therapy had higher salivary IL-6 concentrations than premenopausal women. Alveolar bone densities were also strongly correlated to second metacarpal densities.
  
  
  CONCLUSIONS
  The results of the study suggest that changes in alveolar bone density and levels of bone resorptive cytokines in saliva may be secondary to changes in menopausal status. These changes may predispose loss of alveolar bone with resultant loss of teeth.
authors.affiliation: Department of Diagnostic Sciences, University of Mississippi Medical Center, School of Dentistry, Jackson, USA.
authors.forename: C F
authors.initials: CF
authors.lastname: Streckfus
authors.forename: R B
authors.initials: RB
authors.lastname: Johnson
authors.forename: T
authors.initials: T
authors.lastname: Nick
authors.forename: A
authors.initials: A
authors.lastname: Tsao
authors.forename: M
authors.initials: M
authors.lastname: Tucci
mesh_terms: D000328:Adult; D000368:Aged; D016301:Alveolar Bone Loss / Q000209:etiology*; D000539:Alveolar Process / Q000378:metabolism*; D015519:Bone Density; D015914:Estrogen Replacement Therapy; D005260:Female; D005881:Gingiva / Q000737:chemistry*; D006801:Humans; D015850:Interleukin-6 / Q000032:analysis*; D008593:Menopause / Q000378:metabolism*; D008663:Metacarpus / Q000378:metabolism*; D008875:Middle Aged; D012463:Saliva / Q000737:chemistry*
chemical_list: D015850:Interleukin-6