Competition of plasmid-bearing Pseudomonas putida strains catabolizing naphthalene via various pathways in chemostat culture.
Plasmid-carrying Pseudomonas putida strains degrade naphthalene through different biochemical pathways. The influence of various combinations of host bacteria and plasmids on growth characteristics and competitiveness of P. putida strains was studied in chemostat culture at a low dilution rate (D = 0.05 h-1) with naphthalene as the sole source of carbon and energy. Under naphthalene limitation, the plasmid-bearing strains degrading naphthalene that use catechol 1,2-dioxygenase for catechol oxidation (ortho pathway), were the most competitive. The strains bearing plasmids that control naphthalene catabolism via catechol 2,3-dioxygenase (meta pathway), were less competitive. Under these conditions the strain carrying plasmid pBS4, which encodes for naphthalene catabolism via gentisic acid, was the least competitive.
Filonov
A E
AE
Laboratory of Plasmid Biology, Russian Academy of Sciences, Pushchino, Moscow Region, Russia. FILONOV@IBPM.SERPUKHOV.SU
Duetz
W A
WA
Karpov
A V
AV
Gaiazov
R R
RR
Kosheleva
I A
IA
Breure
A M
AM
Filonova
I F
IF
van Andel
J G
JG
Boronin
A M
AM
D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism*; D001673:Biodegradation, Environmental; D050548:Catechol 1,2-Dioxygenase; D050557:Catechol 2,3-Dioxygenase; D049308:Dioxygenases; D005841:Gentisates; D062385:Hydroxybenzoates / Q000378:metabolism*; D009281:Naphthalenes / Q000378:metabolism*; D010105:Oxygenases / Q000235:genetics / Q000378:metabolism*; D010957:Plasmids / Q000235:genetics; D016958:Pseudomonas putida / Q000235:genetics / Q000378:metabolism*; D013045:Species Specificity
D001426:Bacterial Proteins; D005841:Gentisates; D062385:Hydroxybenzoates; D009281:Naphthalenes; C031721:naphthalene; D010105:Oxygenases; D049308:Dioxygenases; D050548:Catechol 1,2-Dioxygenase; D050557:Catechol 2,3-Dioxygenase; C010925:2,5-dihydroxybenzoic acid