[A study of the effect of the adsorbed molecules of concanavalin A on characteristics of the bound water in the hydrate coat of the disperse silica by 1H NMR of the frozen water suspensions].
The experimental technique is presented which allows the investigation of water at the adsorbent-biopolymer interface by record of temperature dependencies of 1H NMR signals during the heating of previously frozen water suspensions. It was found that the adsorption of protein conconovalin A resulted in twofold decrease of strong bound water amount and in 70% increase of loosely bound one. The mean-value of Gibbs energy decreased twice for strong bound water and increased twice for loosely bound one. The external layer of hydrate shell of silica particles with adsorbed conconovalin A was organized by protein molecules. This conclusion is based on the observed total increase of loosely bound water amount, as a result, of protein adsorption, although its amount with respect to a protein molecule decreased.
Turov
V V
VV
Galagan
N P
NP
Pokrovskaia
O V
OV
Pokrovskiĭ
V A
VA
Dembnovetskaia
L I
LI
D000327:Adsorption; D003208:Concanavalin A / Q000737:chemistry*; D005615:Freezing; D019906:Nuclear Magnetic Resonance, Biomolecular; D011522:Protons; D012822:Silicon Dioxide / Q000737:chemistry*; D013499:Surface Properties; D013535:Suspensions; D013816:Thermodynamics; D014867:Water / Q000737:chemistry*
D011522:Protons; D013535:Suspensions; D014867:Water; D003208:Concanavalin A; D012822:Silicon Dioxide