Sulfated lewis X determinants as a major structural motif in glycans from LS174T-HM7 human colon carcinoma mucin.
This article describes oligosaccharide structures of mucin isolated from nude mouse xenograft tumors produced by LS174T-HM7 cells, a subline of the human colon carcinoma LS174T with higher metastatic tendency and higher mucin production. A striking feature of the oligosaccharides of the LS174T-HM7 xenograft tumor mucin was a predominance of sulfated Lewis X determinants: HSO3-Galbeta1-4(Fucalpha1-3)GlcNAc. In addition to one previously known saccharide with one sulfated Lewis X determinant, the HM7 xenograft tumor mucin contained multiple novel structures containing one, two, or three sulfated Lewis X determinants. This determinant, known to act as a selectin ligand, has been found previously in minor saccharide components of human milk as well as mucins, but never before as a predominant structure in one mucin source.
Capon
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Laboratoire de Chimie Biologique, CNRS Unité Mixte 111, Université des Sciences et Technologies de Lille, 59655 Villeneuve d'Ascq Cedex, France.
Wieruszeski
J M
JM
Lemoine
J
J
Byrd
J C
JC
Leffler
H
H
Kim
Y S
YS
D000818:Animals; D002236:Carbohydrate Conformation; D002240:Carbohydrate Sequence; D003110:Colonic Neoplasms / Q000378:metabolism* / Q000473:pathology; D006801:Humans; D016256:Lewis X Antigen / Q000737:chemistry*; D009682:Magnetic Resonance Spectroscopy; D051379:Mice; D008819:Mice, Nude; D008969:Molecular Sequence Data; D009077:Mucins / Q000737:chemistry*; D011134:Polysaccharides / Q000737:chemistry*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D013402:Sugar Alcohols / Q000138:chemical synthesis; D014407:Tumor Cells, Cultured
D016256:Lewis X Antigen; D009077:Mucins; D011134:Polysaccharides; D013402:Sugar Alcohols