title: Identification of potential ferric binding residues in the iron-binding protein of pathogenic Neisseria meningitidis through structure-based multiple sequence alignments.
abstract: The ferric iron-binding proteins of pathogenic Neisseria display structural and metal-binding properties characteristic of the transferrin family. In the absence of structural data for the ferric iron-binding proteins, spacial folding templates have been derived for the meningococcal protein from complete and partial structure-based multiple sequence alignments with structurally related proteins. The templates have been used to identify a number of potential iron-binding residues. These include four residues that are identical with the iron coordinating ligands of transferrin, but only two reside within equivalent structural elements.
authors.affiliation: Department of Crystallography, Birkbeck College, London, UK. b.gorinsky@iona.cryst.bbk.ac.uk
authors.forename: B
authors.initials: B
authors.lastname: Gorinsky
authors.forename: R W
authors.initials: RW
authors.lastname: Evans
mesh_terms: D001425:Bacterial Outer Membrane Proteins; D001426:Bacterial Proteins / Q000737:chemistry*; D001665:Binding Sites; D005290:Ferric Compounds / Q000737:chemistry*; D033862:Iron-Binding Proteins; D008958:Models, Molecular; D008969:Molecular Sequence Data; D009345:Neisseria meningitidis / Q000737:chemistry*; D033902:Periplasmic Binding Proteins; D010710:Phosphates / Q000737:chemistry; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid; D013329:Structure-Activity Relationship
chemical_list: D001425:Bacterial Outer Membrane Proteins; D001426:Bacterial Proteins; D005290:Ferric Compounds; D033862:Iron-Binding Proteins; D033902:Periplasmic Binding Proteins; D010710:Phosphates