title: HLA-D types and serum IgG responses to Capnocytophaga in diabetes and periodontitis.
abstract: Serum IgG responses to the cell envelope proteins (CEPs) from Capnocytophaga sputigena, Capnocytophaga ochracea, and Capnocytophaga gingivalis were examined in periodontally healthy and periodontitis subjects, both with and without type 1 diabetes (n = 60). Serum IgG responses to CEPs were determined by immunoblotting with biotin-goat anti-human IgG and an alkaline phosphatase-streptavidin system. Reactivity was analyzed by transmission densitometry, digitization, and computer manipulation. The patients with diabetes showed significantly (p < 0.01) fewer responses to 14 CEPs (from 81 to 10 kDa) from C. sputigena, 5 CEPs (from 90 to 17 kDa) from C. gingivalis, and the 27-kDa CEP from C. ochracea than in the non-diabetic group. The periodontitis patients showed significantly (p < 0.01) fewer responses to the 25- and 11-kDa CEPs from C. sputigena, the 125- and 17-kDa CEPs from C. gingivalis, and the 42-kDa CEP from C. ochracea than in the periodontally healthy group. HLA-DR4, HLA-DR53, and HLA-DQw3 were associated with periodontitis, while only HLA-DR4 was associated with diabetes (p < 0.02). Significant (p < 0.01) correlations were found between HLA-DR2 and IgG reactivity patterns associated with non-diabetics, and between HLA-DR4 and IgG reactivity patterns associated with diabetic and periodontitis subjects. These results indicate that both type 1 diabetics and periodontitis subjects have a depressed IgG antibody profile to Capnocytophaga, which may account for an increased susceptibility to periodontitis infection. Periodontitis in type 1 diabetes may be related more to the HLA-D type and altered immune function than to the diabetes itself.
authors.affiliation: Department of Oral Biology, University of Nebraska Medical Center, College of Dentistry, Lincoln 68583-0740, USA.
authors.forename: J K
authors.initials: JK
authors.lastname: Dyer
authors.forename: M A
authors.initials: MA
authors.lastname: Peck
authors.forename: R A
authors.initials: RA
authors.lastname: Reinhardt
authors.forename: W C
authors.initials: WC
authors.lastname: Duckworth
authors.forename: S J
authors.initials: SJ
authors.lastname: Booth
authors.forename: G J
authors.initials: GJ
authors.lastname: Seymour
authors.forename: K D
authors.initials: KD
authors.lastname: Patil
mesh_terms: D000328:Adult; D000907:Antibodies, Bacterial / Q000097:blood*; D000937:Antigen-Antibody Reactions; D001425:Bacterial Outer Membrane Proteins / Q000276:immunology; D002206:Capnocytophaga / Q000276:immunology*; D003922:Diabetes Mellitus, Type 1 / Q000150:complications / Q000276:immunology*; D004198:Disease Susceptibility; D004591:Electrophoresis, Polyacrylamide Gel; D005260:Female; D006681:HLA-D Antigens / Q000097:blood*; D006801:Humans; D015151:Immunoblotting; D007074:Immunoglobulin G / Q000097:blood*; D008297:Male; D010518:Periodontitis / Q000209:etiology / Q000276:immunology*
chemical_list: D000907:Antibodies, Bacterial; D001425:Bacterial Outer Membrane Proteins; D006681:HLA-D Antigens; D007074:Immunoglobulin G