Hyaluronic acid suppresses fibronectin fragment mediated cartilage chondrolysis: II. In vivo.
Intra-articular sodium hyaluronic acid (HA) has been used as a treatment intervention in the management of osteoarthritis. It has been observed that HA can coat the articular surface, and thus, has been suggested to provide a possible prophylactic barrier for the articular cartilage. In an accompanying manuscript (Homandberg et al.), we report that a commercially available high-molecular-weight HA (approximately 800-kDa, ARTZ, Seikagaku Corp.) can partially block fibronectin fragment (Fn-f)-mediated cartilage injury in vitro. Herein we report a study of the effects of intra-articular HA on an in vivo animal model of Fn-f-mediated cartilage injury. Rabbit knees were injected with Fn-f, and after 1 week, the cartilage proteoglycan (PG) content had decreased to 59 +/- 8% of control. In sharp contrast, PG content in knees receiving pre-treatment with HA followed by Fn-f injection had only decreased to 85 +/- 27% of control (P < 0.01). Similarly, the PG content in knees receiving an injection of Fn-f, followed by an injection of HA were significantly higher (74 +/- 18% of control) than Fn-f injured knees with no treatment (P < 0.02). Intra-articular HA alone had no effect on cartilage PG content. The results in this study suggest that HA is effective in partially preventing Fn-f mediated cartilage injury, most likely by coating the articular surface. Further, HA treatment after Fn-f injury may facilitate restoration of matrix components.
Williams
J M
JM
Department of Anatomy, Rush Medical College, Rush Presbyterian St. Luke's Medical Center, Chicago, Illinois 60612-3864, USA.
Plaza
V
V
Hui
F
F
Wen
C
C
Kuettner
K E
KE
Homandberg
G A
GA
D000818:Animals; D002358:Cartilage, Articular / Q000187:drug effects* / Q000378:metabolism; D005353:Fibronectins / Q000008:administration & dosage*; D006820:Hyaluronic Acid / Q000494:pharmacology*; D007719:Knee Joint; D008297:Male; D010446:Peptide Fragments / Q000008:administration & dosage*; D011817:Rabbits
D005353:Fibronectins; D010446:Peptide Fragments; D006820:Hyaluronic Acid