Generation of large insert yeast artificial chromosome libraries.
The development of YAC cloning technology has directly enhanced the relationship among genetic, physical, and functional mapping of genomes. Because of their large size, YACs have enabled the rapid construction of physical maps by ordered clone mapping and contig building, and they complement other molecular approaches for mapping complex genomes. Large insert libraries are constructed by size fractionating large DNA embedded in agarose and protecting DNA from degradation with polyamines.
Larin
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Z
Institute of Molecular Medicine, University of Oxford, John Radcliffe Hospital, Headington, UK. zlarin@worfmolbiol.ex.ac.uk
Monaco
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AP
Lehrach
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H
D018244:Chromosomes, Artificial, Yeast; D016521:Electrophoresis, Gel, Pulsed-Field / Q000379:methods; D015723:Gene Library; D005822:Genetic Vectors; D008967:Molecular Biology / Q000379:methods*; D014170:Transformation, Genetic