Genome methylation of the marine annelid worm Chaetopterus variopedatus: methylation of a CpG in an expressed H1 histone gene.
Hydrolysis by methylation-dependent restriction enzymes shows that the genomic DNA of the polychaete annelid worm Chaetopterus variopedatus is methylated. Electrophoretic analyses of the digestion products indicate that the degree of methylation is lower in adult tissues than in sperm and embryonic DNA. 5-Methylcytosine was identified by HPLC, absorption spectroscopy and mass spectrometry analyses of free bases obtained by acid hydrolysis of the DNA. An average value of 1.6% methylated cytosines was determined in sperm DNA. Partial methylation was also found in an actively expressed H1 histone gene. This is the first time that genomic DNA methylation is demonstrated to occur in a worm.
del Gaudio
R
R
Department of Genetics, General and Molecular Biology, University of Naples Federico II, Italy.
Di Giaimo
R
R
Geraci
G
G
D000818:Animals; D015152:Blotting, Northern; D018899:CpG Islands; D019175:DNA Methylation; D015257:DNA-Cytosine Methylases / Q000378:metabolism; D005260:Female; D015870:Gene Expression; D006657:Histones / Q000235:genetics*; D008297:Male; D013058:Mass Spectrometry; D011077:Polychaeta / Q000235:genetics*
D006657:Histones; C029641:DNA modification methylase HpaII; D015257:DNA-Cytosine Methylases