Antisense inhibition of the photosystem I antenna protein Lhca4 in Arabidopsis thaliana.
The function of Lhca4, a gene encoding the photosystem 1 type IV chlorophyll a/b-binding protein complex in Arabidopsis, was investigated using antisense technology. Lhca4 protein was reduced in a number of mutant lines and abolished in one. The inhibition of protein was not correlated with the inhibition of mRNA. No depletion of Lhca1 was observed, but the low-temperature fluorescence emission spectrum was drastically altered in the mutants. The emission maximum was blue-shifted by 6 nm, showing that chlorophyll molecules bound to Lhca4 are responsible for most of the long-wavelength fluorescence emission. Some mutants also showed an unexplainable delay in flowering time and an increase in seed weight.
Zhang
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Department of Biological Sciences, Texas Tech University, Lubbock 79409, USA. brahz@ttacs.ttu.edu
Goodman
H M
HM
Jansson
S
S
D017360:Arabidopsis / Q000187:drug effects / Q000235:genetics / Q000378:metabolism*; D002734:Chlorophyll / Q000378:metabolism; D061146:Chlorophyll Binding Proteins; D018506:Gene Expression Regulation, Plant / Q000187:drug effects*; D045342:Light-Harvesting Protein Complexes; D016296:Mutagenesis; D016376:Oligonucleotides, Antisense / Q000494:pharmacology*; D010641:Phenotype; D045322:Photosynthetic Reaction Center Complex Proteins / Q000096:biosynthesis* / Q000235:genetics; D045331:Photosystem I Protein Complex; D010940:Plant Proteins / Q000096:biosynthesis* / Q000235:genetics; D030821:Plants, Genetically Modified; D012333:RNA, Messenger / Q000096:biosynthesis
D061146:Chlorophyll Binding Proteins; D045342:Light-Harvesting Protein Complexes; D016376:Oligonucleotides, Antisense; D045322:Photosynthetic Reaction Center Complex Proteins; D045331:Photosystem I Protein Complex; D010940:Plant Proteins; D012333:RNA, Messenger; D002734:Chlorophyll