Mutational analysis of pcpA and its role in pentachlorophenol degradation by Sphingomonas (Flavobacterium) chlorophenolica ATCC 39723.
Sphingomonas (Flavobacterium) chlorophenolica ATCC 39723 degrades pentachlorophenol (PCP) through a catabolic pathway encoded by multiple genes. One gene required for PCP degradation is pcpA, which encodes information for a 30-kDa polypeptide, PcpA, found in the periplasm of the bacterium. The biological role of PcpA has remained unknown. We disrupted pcpA by replacing it with a defective copy through homologous recombination. The pcpA recombinant, mutant strains accumulated 2,6-dichlorohydroquinone (2,6-DiCH) as a metabolite of PCP. This work confirms that pcpA is essential for degradation of PCP by S. chlorophenolica ATCC 39723 and suggests that it encodes a protein involved in hydrolytic dehalogenation of 2,6-DiCH, an already established primary metabolite of the PCP catabolic pathway.
Chanama
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University of Idaho Institute for Molecular and Agricultural Genetic Engineering, Moscow 83844-1052, USA.
Crawford
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RL
D001426:Bacterial Proteins / Q000235:genetics* / Q000378:metabolism*; D001483:Base Sequence; D001673:Biodegradation, Environmental; D017931:DNA Primers / Q000235:genetics; D004785:Environmental Pollutants / Q000378:metabolism*; D005417:Flavobacterium / Q000235:genetics* / Q000378:metabolism*; D008401:Gas Chromatography-Mass Spectrometry; D005798:Genes, Bacterial; D006873:Hydroquinones / Q000378:metabolism; D009154:Mutation; D010416:Pentachlorophenol / Q000737:chemistry / Q000378:metabolism*
D001426:Bacterial Proteins; D017931:DNA Primers; D004785:Environmental Pollutants; D006873:Hydroquinones; D010416:Pentachlorophenol