Expression of human immunodeficiency virus genes using baculovirus expression system.
The structural protein genes of HIV-1 and HIV-2 have been expressed in Spodoptera frugiperda (SF) cells using baculovirus expression system. The noncoding flanking sequences of HIV structural genes were removed and a putative ribosome binding site was placed in front of the open reading frame of each gene by using crossover linker mutagenesis. The coding sequences of the gag, pol, env, and vif proteins were inserted into Autographa californica nuclear polyhedrosis virus (AcNPV) so that HIV genes were under the control of the AcNPV polyhedrin promoter. All recombinant AcNPV-infected SF cells express high levels of HIV structural proteins. Detailed strategies of recombinant AcNPV construction for high level protein expression are presented.
Kang
C Y
CY
Faculty of Science, Western Science Centre, University of Western Ontario, London, Canada. scicyk@uwoadmin.uwo.ca
D000818:Animals; D016367:Baculoviridae / Q000235:genetics*; D002478:Cells, Cultured; D015683:Gene Products, gag / Q000096:biosynthesis / Q000235:genetics; D015684:Gene Products, pol / Q000096:biosynthesis / Q000235:genetics; D016341:Genes, vif; D006678:HIV / Q000235:genetics*; D015699:HIV Envelope Protein gp120 / Q000096:biosynthesis / Q000235:genetics; D015497:HIV-1 / Q000235:genetics; D015498:HIV-2 / Q000235:genetics; D015202:Protein Engineering / Q000379:methods*; D011498:Protein Precursors / Q000096:biosynthesis / Q000235:genetics; D011994:Recombinant Proteins / Q000096:biosynthesis* / Q000235:genetics; D018411:Spodoptera / Q000166:cytology / Q000821:virology; D014764:Viral Proteins / Q000096:biosynthesis / Q000235:genetics; D054301:gag Gene Products, Human Immunodeficiency Virus; D054302:pol Gene Products, Human Immunodeficiency Virus
D015683:Gene Products, gag; D015684:Gene Products, pol; D015699:HIV Envelope Protein gp120; C060467:HIV pol protein p65; D011498:Protein Precursors; D011994:Recombinant Proteins; D014764:Viral Proteins; D054301:gag Gene Products, Human Immunodeficiency Virus; C094860:gag precursor protein, HIV-2; D054302:pol Gene Products, Human Immunodeficiency Virus