A new MHC locus that influences class I peptide presentation.
We have investigated the HLA-B27-restricted CTL response to HY minor histocompatibility antigens in rats and mice transgenic for HLA-B27 and human beta2-microglobulin. A polymorphism was found at a locus within the H2 complex, producing two distinct but overlapping sets of B27-presented HY peptides. The locus, named Cim2, mapped between the K and Pb loci, and its product is therefore distinct from TAP, LMP, and tapasin. Identical findings in rats and mice, including identical HY peptide sequences and the failure of a rat Tap2A transgene to alter CTL recognition, suggest that a homologous locus with similar polymorphism exists in the rat. Cim2, or a closely linked locus, was found to exert a broad effect on peptide loading of both HLA-B27 and mouse class I alleles. The data thus establish a strong, previously unrecognized MHC-encoded influence on the class I antigen pathway.
Simmons
W A
WA
Harold C. Simmons Arthritis Research Center, Department of Internal Medicine, University of Texas Southwestern Medical Center, Dallas 75235, USA.
Roopenian
D C
DC
Summerfield
S G
SG
Jones
R C
RC
Galocha
B
B
Christianson
G J
GJ
Maika
S D
SD
Zhou
M
M
Gaskell
S J
SJ
Bordoli
R S
RS
Ploegh
H L
HL
Slaughter
C A
CA
Lindahl
K F
KF
Hammer
R E
RE
Taurog
J D
JD
D000071450:ATP Binding Cassette Transporter, Subfamily B, Member 3; D018528:ATP-Binding Cassette Transporters / Q000502:physiology; D000818:Animals; D030801:Animals, Genetically Modified; D002874:Chromosome Mapping; D003602:Cytotoxicity, Immunologic; D006182:H-Y Antigen / Q000276:immunology; D015796:HLA-B27 Antigen / Q000276:immunology; D015395:Histocompatibility Antigens Class I / Q000276:immunology*; D006801:Humans; D007111:Immunity, Cellular; D008285:Major Histocompatibility Complex; D051379:Mice; D010455:Peptides / Q000276:immunology; D011110:Polymorphism, Genetic; D051381:Rats; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology*
D000071450:ATP Binding Cassette Transporter, Subfamily B, Member 3; D018528:ATP-Binding Cassette Transporters; D006182:H-Y Antigen; D015796:HLA-B27 Antigen; D015395:Histocompatibility Antigens Class I; D010455:Peptides; C489516:Tap2 protein, mouse; C489514:Tap2 protein, rat; C071692:TAP2 protein, human