Promoters of epithelialization induce expression of vascular endothelial growth factor in human gastric epithelial cells in primary culture.
Both epithelialization and angiogenesis are indispensable processes in gastric ulcer healing. Coordination between these processes has not been well studied. In the present study, we have established a new primary culture system of human gastric epithelial cells and investigated the effect of epithelialization stimulants on a specific angiogenic factor, vascular endothelial growth characterized as epithelial cells. Both epithelialization stimulants, hepatocyte growth factor (HGF) and epidermal growth factor (EGF), significantly stimulated vascular EGF expression in gastric epithelial cells. HGF and EGF receptors were expressed by the cells, suggesting that regulation may be mediated through specific receptors.
Takahashi
M
M
Second Department of Internal Medicine, Faculty of Medicine, University of Tokyo, Japan. TAKAHASHI-2IM@H.U-TOKYO.AC.JP
Ogura
K
K
Maeda
S
S
Mori
K
K
Mafune
K
K
Mikami
Y
Y
Terano
A
A
Omata
M
M
D000328:Adult; D017398:Alternative Splicing; D002478:Cells, Cultured; D017931:DNA Primers; D016228:Endothelial Growth Factors / Q000096:biosynthesis*; D004815:Epidermal Growth Factor / Q000494:pharmacology*; D066246:ErbB Receptors / Q000096:biosynthesis; D005753:Gastric Mucosa / Q000166:cytology / Q000187:drug effects / Q000502:physiology*; D014644:Genetic Variation; D017228:Hepatocyte Growth Factor / Q000494:pharmacology*; D006801:Humans; D008222:Lymphokines / Q000096:biosynthesis*; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000096:biosynthesis; D014158:Transcription, Genetic / Q000187:drug effects; D042461:Vascular Endothelial Growth Factor A; D042442:Vascular Endothelial Growth Factors
D017931:DNA Primers; D016228:Endothelial Growth Factors; D008222:Lymphokines; D012333:RNA, Messenger; D042461:Vascular Endothelial Growth Factor A; D042442:Vascular Endothelial Growth Factors; D004815:Epidermal Growth Factor; D017228:Hepatocyte Growth Factor; D066246:ErbB Receptors