Long-term resistance to HIV infection in vertical HIV infection: cytokine production, HIV isolation, and HIV phenotype define long-term resistant hosts.
We analyzed immunologic (CD4 and CD8 slopes; interferon-gamma, interleukin-2, interleukin-10, and chemokines production; concentration of IgE; beta 2-microglobulin) and virologic (p24; HIV isolability and phenotype; plasma viremia) parameters in HIV vertically infected children > or = 8 years of age without disease progression or mild symptoms and an absolute CD4+ count > or = 500/microliter with CD4+ percentage > or = 25%. The results were compared to those of two control groups: (1) slow progressors, children > or = 8 years of age with moderate symptomatology and/or moderate CD4 depletion, and (2) progressors, children > or = 8 years of age with severe clinical disease and/or severe CD4 depletion. Pediatric long-term resistant hosts were characterized by higher production of interleukin-2 and interferon-gamma and lower production of interleukin-10, normal concentration of IgE, HIV isolates with a non-syncytium-inducing phenotype, and lower plasma viremia. This condition was not associated with the concentration of beta 2-microglobulin, p24, and chemokines, or with HIV isolability. The IL-10/IL-2 ratio best correlated with both CD4 counts and disease progression. Thus, vertically infected children showing resistance to disease progression are immunologically and virologically distinct from those in whom progressive HIV infection is observed.
Vigano
A
A
Cattedra di Pediatria IV, H.L. Sacco, Università di Milano, Italia.
Balotta
C
C
Trabattoni
D
D
Bricalli
D
D
Crupi
L
L
Palomba
E
E
Galli
L
L
Salvaggio
A
A
Fusi
M L
ML
Ruzzante
S
S
Massironi
E
E
Colombo
M C
MC
Principi
N
N
Galli
M
M
Clerici
M
M
D000293:Adolescent; D018791:CD4 Lymphocyte Count; D016516:CD4-CD8 Ratio; D054407:Chemokine CCL4; D018946:Chemokine CCL5 / Q000378:metabolism; D002648:Child; D016207:Cytokines / Q000378:metabolism*; D006678:HIV / Q000235:genetics / Q000276:immunology / Q000302:isolation & purification*; D015658:HIV Infections / Q000276:immunology* / Q000635:transmission*; D006801:Humans; D007113:Immunity, Innate / Q000276:immunology; D007073:Immunoglobulin E / Q000097:blood; D018445:Infectious Disease Transmission, Vertical; D007371:Interferon-gamma / Q000378:metabolism; D016753:Interleukin-10 / Q000378:metabolism; D007376:Interleukin-2 / Q000378:metabolism; D007963:Leukocytes, Mononuclear / Q000187:drug effects / Q000378:metabolism; D019402:Macrophage Inflammatory Proteins / Q000378:metabolism; D010641:Phenotype; D012367:RNA, Viral / Q000032:analysis
D054407:Chemokine CCL4; D018946:Chemokine CCL5; D016207:Cytokines; D007376:Interleukin-2; D019402:Macrophage Inflammatory Proteins; D012367:RNA, Viral; D016753:Interleukin-10; D007073:Immunoglobulin E; D007371:Interferon-gamma