title: Pyrimidine biosynthesis in Pseudomonas stutzeri ATCC 17588.
abstract: The de novo pyrimidine biosynthetic enzymes in the denitrifying bacterium Pseudomonas stutzeri ATCC 17588 were assayed and their activities were lower in glucose-grown cells than in succinate-grown cells. When P. stutzeri was grown in the presence of uracil, the de novo enzyme activities in succinate-grown cells were lowered while they remained largely unchanged in glucose-grown cells. A uracil auxotroph of P. stutzeri, deficient for aspartate transcarbamoylase activity, was isolated and its auxotrophic requirement was met by only uracil and cytosine. The inability of pyrimidine ribonucleosides to meet the auxotrophic requirement was related to the limited ability of P. stutzeri to transport uridine and cytidine. Pyrimidine limitation of the auxotroph elevated the de novo enzyme activities indicating that this pathway may be repressible by a uracil-related compound in succinate-grown P. stutzeri cells. Regulation of pyrimidine synthesis in P. stutzeri was similar to that observed for other pseudomonads classified within rRNA homology group I.
authors.affiliation: Olson Biochemistry Laboratories, Department of Chemistry and Biochemistry, South Dakota State University, Brookings 57007, USA.
authors.forename: T P
authors.initials: TP
authors.lastname: West
mesh_terms: D001221:Aspartate Carbamoyltransferase / Q000172:deficiency / Q000235:genetics / Q000378:metabolism; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism; D003470:Culture Media / Q000494:pharmacology; D003596:Cytosine / Q000096:biosynthesis; D004080:Dihydroorotase / Q000378:metabolism; D000090722:Dihydroorotate Dehydrogenase; D005947:Glucose / Q000494:pharmacology; D009962:Orotate Phosphoribosyltransferase / Q000378:metabolism; D009964:Orotidine-5'-Phosphate Decarboxylase / Q000378:metabolism; D010088:Oxidoreductases / Q000378:metabolism; D044925:Oxidoreductases Acting on CH-CH Group Donors; D011549:Pseudomonas / Q000187:drug effects / Q000201:enzymology / Q000378:metabolism*; D011743:Pyrimidines / Q000096:biosynthesis*; D013045:Species Specificity; D019802:Succinic Acid / Q000494:pharmacology; D014498:Uracil / Q000096:biosynthesis
chemical_list: D001426:Bacterial Proteins; D003470:Culture Media; D000090722:Dihydroorotate Dehydrogenase; D011743:Pyrimidines; D014498:Uracil; D003596:Cytosine; D019802:Succinic Acid; D010088:Oxidoreductases; D044925:Oxidoreductases Acting on CH-CH Group Donors; D001221:Aspartate Carbamoyltransferase; D009962:Orotate Phosphoribosyltransferase; D004080:Dihydroorotase; D009964:Orotidine-5'-Phosphate Decarboxylase; D005947:Glucose