Longitudinal study of Aspergillus fumigatus strains isolated from cystic fibrosis patients.
The colonization over time of cystic fibrosis patients by Aspergillus fumigatus was investigated using a DNA fingerprinting method. Aspergillus fumigatus isolates collected sequentially for more than one year from six patients with cystic fibrosis were typed by Southern blot hybridization with a repetitive DNA sequence. Each cystic fibrosis patient harbored several strains of Aspergillus fumigatus that were isolated recurrently over time. Isolates collected from a cystic fibrosis patient with aspergilloma displayed the same genotype, suggesting that the infection was due to a single strain. Continuous isolation of the same genotype in another cystic fibrosis patient, however, was not correlated clinically with an Aspergillus infection.
Neuvéglise
C
C
Laboratoire des Aspergillus, Institut Pasteur, Paris, France.
Sarfati
J
J
Debeaupuis
J P
JP
Vu Thien
H
H
Just
J
J
Tournier
G
G
Latgé
J P
JP
D000293:Adolescent; D001228:Aspergillosis / Q000453:epidemiology* / Q000235:genetics* / Q000517:prevention & control; D001232:Aspergillus fumigatus / Q000235:genetics / Q000302:isolation & purification*; D002648:Child; D002675:Child, Preschool; D003550:Cystic Fibrosis / Q000150:complications*; D016172:DNA Fingerprinting; D004271:DNA, Fungal / Q000032:analysis*; D005260:Female; D006801:Humans; D008137:Longitudinal Studies; D008297:Male; D017720:Molecular Epidemiology; D012008:Recurrence; D013997:Time Factors
D004271:DNA, Fungal