Cell cycle dependent toxicity of an amphiphilic synthetic peptide.
The cytotoxic properties of an amphiphilic synthetic peptide are presented. Comparative analysis of proliferating, differentiated and confluent H9C2 adherent cells and L1210 cells in suspension shows a correlation between toxicity and cell stage (proliferating cells). Electrophysiological measurements on Xenopus laevis oocytes bathed in the peptide also demonstrated the induction of cationic currents, which is voltage and phosphate dependent. These results allow us to hypothesize that the observed toxicity is related to membrane hyperpolarization of proliferating cells at the G1/S cell cycle phase transition.
Pellegrin
P
P
Centre de Recherche de Biochimie Macromoléculaire, Montpellier, France. ppellegr@vega.crbm.cnrs-mop.fr
Menard
C
C
Mery
J
J
Lory
P
P
Charnet
P
P
Bennes
R
R
D000595:Amino Acid Sequence; D000818:Animals; D002453:Cell Cycle / Q000502:physiology*; D002455:Cell Division; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects*; D002942:Circular Dichroism; D005260:Female; D007939:Leukemia L1210; D008564:Membrane Potentials / Q000187:drug effects; D051379:Mice; D008969:Molecular Sequence Data; D009865:Oocytes / Q000187:drug effects / Q000502:physiology*; D010455:Peptides / Q000737:chemistry* / Q000494:pharmacology*; D010715:Phosphatidylglycerols; D051381:Rats; D014407:Tumor Cells, Cultured; D014982:Xenopus laevis
D010455:Peptides; D010715:Phosphatidylglycerols; C051388:1,2-dioleoyl-sn-glycero-3-phosphoglycerol