A chromatin-associated kinesin-related protein required for normal mitotic chromosome segregation in Drosophila.
The tiovivo (tio) gene of Drosophila encodes a kinesin-related protein, KLP38B, that colocalizes with condensed chromatin during cell division. Wild-type function of the tio gene product KLP38B is required for normal chromosome segregation during mitosis. Mitotic cells in tio larval brains displayed circular mitotic figures, increased ploidy, and abnormal anaphase figures. KLP38B mRNA is maternally provided and expressed in cells about to undergo division. We propose that KLP38B, perhaps redundantly with other chromosome-associated microtubule motor proteins, contributes to interactions between chromosome arms and microtubules important for establishing bipolar attachment of chromosomes and assembly of stable bipolar spindles.
Molina
I
I
Centro de Biología Molecular "Severo Ochoa" Consejo Superior Investigaciones Científicas-Universidad Autónoma Madrid, Facultad de Ciencias, UAM, Cantoblanco, 28049 Madrid, Spain.
Baars
S
S
Brill
J A
JA
Hales
K G
KG
Fuller
M T
MT
Ripoll
P
P
D000595:Amino Acid Sequence; D000705:Anaphase; D000818:Animals; D001921:Brain / Q000166:cytology / Q000378:metabolism; D002455:Cell Division; D002843:Chromatin / Q000502:physiology* / Q000648:ultrastructure; D002875:Chromosomes / Q000502:physiology*; D004330:Drosophila / Q000235:genetics*; D029721:Drosophila Proteins; D017344:Genes, Insect; D019476:Insect Proteins / Q000096:biosynthesis / Q000235:genetics; D007438:Introns; D016547:Kinesins / Q000096:biosynthesis / Q000737:chemistry / Q000235:genetics*; D007814:Larva; D008938:Mitosis; D008969:Molecular Sequence Data; D011003:Ploidies; D012333:RNA, Messenger / Q000096:biosynthesis; D015183:Restriction Mapping; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid; D014158:Transcription, Genetic
D002843:Chromatin; D029721:Drosophila Proteins; D019476:Insect Proteins; D012333:RNA, Messenger; C106739:neb protein, Drosophila; D016547:Kinesins