Definite-clause grammars for the analysis of cis-regulatory regions in E. coli.
Based on an extensive collection of sigma 70 associated regulatory mechanisms, a grammatical model has been constructed that define the functional positions and combinations of sites within DNA regulatory regions. The syntactic rules and the dictionary implemented in a Prolog program were coupled to consensus matrices used as "sensors" to integrate a syntactic recognizer. A systematic comparison between the syntactic recognizer and the standard weight matrix methodology is presented using 12 regulatory proteins and the whole collection of about 130 sigma 70 DNA regulatory regions. On the average an increased sensitivity of 5 to 10 fold is obtained with this novel approach.
Thieffry
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Centro de Investigación sobre Fijación de Nitrógeno, Universidad Nacional Autónoma de México, Cuernavaca, Morelos, México.
Rosenblueth
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DA
Huerta
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AM
Salgado
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Collado-Vides
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D001665:Binding Sites; D016384:Consensus Sequence; D004269:DNA, Bacterial / Q000737:chemistry* / Q000235:genetics*; D004268:DNA-Binding Proteins / Q000737:chemistry / Q000378:metabolism; D012321:DNA-Directed RNA Polymerases / Q000737:chemistry* / Q000378:metabolism*; D016208:Databases, Factual; D004926:Escherichia coli / Q000235:genetics* / Q000378:metabolism; D011381:Programming Languages; D011401:Promoter Regions, Genetic; D012045:Regulatory Sequences, Nucleic Acid; D012808:Sigma Factor / Q000737:chemistry* / Q000378:metabolism*
D004269:DNA, Bacterial; D004268:DNA-Binding Proteins; D012808:Sigma Factor; C081775:RNA polymerase sigma 70; D012321:DNA-Directed RNA Polymerases