title: Aqueous-based microcapsules are detected primarily in gut-associated dendritic cells after oral inoculation of mice.
abstract: We previously found that aqueous-based microencapsulation enhanced virus-specific humoral immune responses after oral inoculation of mice. However, the mechanism by which microencapsulation enhances immunogenicity remains unclear. We found that spermine-alginate microcapsules were detected primarily in gut-associated dendritic cells (i.e. CD11c/CD18+, Ia+, CD11b-, CD45R-) after oral inoculation of adult mice. Microencapsulation may enhance immunogenicity by involving antigen presenting cells which are more efficient than those recruited during natural infection.
authors.affiliation: Section of Infectious Diseases, Children's Hospital of Philadelphia, PA, USA.
authors.forename: E A
authors.initials: EA
authors.lastname: Lomotan
authors.forename: K A
authors.initials: KA
authors.lastname: Brown
authors.forename: T J
authors.initials: TJ
authors.lastname: Speaker
authors.forename: P A
authors.initials: PA
authors.lastname: Offita
mesh_terms: D000284:Administration, Oral; D000464:Alginates / Q000493:pharmacokinetics*; D000818:Animals; D002214:Capsules; D003713:Dendritic Cells / Q000378:metabolism*; D005260:Female; D020723:Glucuronic Acid; D006603:Hexuronic Acids; D007413:Intestinal Mucosa / Q000378:metabolism; D007422:Intestines / Q000166:cytology* / Q000276:immunology; D008221:Lymphoid Tissue / Q000166:cytology* / Q000276:immunology / Q000378:metabolism; D051379:Mice; D012235:Rhodamines / Q000493:pharmacokinetics; D013096:Spermine / Q000493:pharmacokinetics*; D013154:Spleen / Q000378:metabolism; D014867:Water
chemical_list: D000464:Alginates; D002214:Capsules; D006603:Hexuronic Acids; D012235:Rhodamines; D014867:Water; D013096:Spermine; D020723:Glucuronic Acid