The processing of DNA ends at double-strand breaks during homologous recombination: different roles for the two ends.
We have investigated the role of DNA ends during gap repair by homologous recombination. Mouse cells were transfected with a gapped plasmid carrying distinctive ends: on one side mouse LINE-1 repetitive sequences (L1Md-A2), and on the other rat LINE-1 sequences (L1Rn-3). The gap could be repaired by homologous recombination with endogenous mouse genomic LINE-1 elements, which are on average 95% and 85% homologous to L1Md-A2 and L1Rn-3 ends, respectively. Both L1Md-A2 and L1Rn-3 ends were found to initiate gap repair with equal efficiency. However, there were two types of gap repair products--precise and imprecise--the occurrence of which appears to depend on which end had been used for initiation and thus which end was left available for subsequent steps in recombination. These results, together with sequence analysis of recombinants obtained with plasmids having either mouse or rat LINE-1 sequences flanking the gap, strongly suggest that the two DNA ends played different roles in recombinational gap repair. One end was used to initiate the gap repair process, while the other end was involved at later steps, in the resolution of the recombination event.
Villemure
J F
JF
Institut du cancer de Montréal, Université de Montréal, Québec, Canada.
Belmaaza
A
A
Chartrand
P
P
D000818:Animals; D004247:DNA / Q000378:metabolism*; D004249:DNA Damage; D004260:DNA Repair; D051379:Mice; D010957:Plasmids; D051381:Rats; D011995:Recombination, Genetic; D012091:Repetitive Sequences, Nucleic Acid
D004247:DNA