title: Screening for EDTA-dependent deviations in platelet counts and abnormalities in platelet distribution histograms in pseudothrombocytopenia.
abstract: Screening for pseudothrombocytopenia caused by in vitro platelet clumping has been performed in 45,000 subjects attending a general hospital. In our region, the observed prevalence of EDTA-induced pseudothrombocytopenia in blood samples with an initial platelet count below 150 x 10(9)/l was estimated to amount to 0.1%. EDTA-induced pseudothrombocytopenia was confirmed by detection of platelet aggregates by means of microscopic evaluation from the blood smear. In routine investigations, pseudothrombocytopenia could be highly suspected when the Sysmex NE 8000 showed characteristic peculiarities in the white blood cell (WBC) scattergram and histogram. Platelet aggregation is avoided in such cases by the use of citrate as an anticoagulant instead of EDTA. Pseudothrombocytopenia was detected in 46 subjects. As a screening test for pseudothrombocytopenia, increased cut-off values derived from the WBC histogram demonstrated 90% sensitivity and 100% specificity. Automated flagging for platelet clumps, deviations reflecting MPV, or PDW abnormalities revealed lower scores with respect to sensitivity.
authors.affiliation: Department of Clinical Chemistry, Haematology and Immunology, Medical Centre Alkmaar, The Netherlands.
authors.forename: P C
authors.initials: PC
authors.lastname: Bartels
authors.forename: M
authors.initials: M
authors.lastname: Schoorl
authors.forename: A J
authors.initials: AJ
authors.lastname: Lombarts
mesh_terms: D000925:Anticoagulants; D001792:Blood Platelets / Q000166:cytology; D048429:Cell Size; D002614:Chelating Agents; D019343:Citric Acid; D003951:Diagnostic Errors; D004492:Edetic Acid; D006801:Humans; D007958:Leukocyte Count; D010974:Platelet Aggregation; D010976:Platelet Count / Q000379:methods*; D013921:Thrombocytopenia / Q000175:diagnosis*
chemical_list: D000925:Anticoagulants; D002614:Chelating Agents; D019343:Citric Acid; D004492:Edetic Acid