Contents of resveratrol, piceid, and their isomers in commercially available wines made from grapes cultivated in Japan.
The presence of resveratrol (3,5,4'-trihydroxystilbene) and its beta-glucoside, piceid (resveratrol-3-beta-D-glucopyranoside), together with their isomers in wine appears to be one of the beneficial factors conferring a protective effect against cardiovascular disease through red wine ingestion. A total of 42 red and white wines was collected in areas from Hokkaido to Kyushu in Japan. The wines were fractionated with a C18 Sep-pak cartridge, and the active principles were eluted with ethyl acetate. Crude trans- and cis-piceid were extracted from a Chinese medicine, 'Kojohkon' (Polygonum cuspidatum), and their retention times and UV absorption were confirmed by HPLC. trans- and cis-Resveratrol, and trans- and cis-piceid were analyzed in a short C18 HPLC column, and cis-resveratrol was quantified from the amount of cis-isomer converted from authentic trans-resveratrol that had been treated by UV irradiation. The content of piceid is shown as the resveratrol equivalent. The average content of total stilbene compounds was 4.37 mg/liter in red wines, while only 0.68 mg/liter in white wines. Red wines made from Pinot noir, Merlot, and Zweigeltrebe grapes all had a high resveratrol content.
Sato
M
M
Wines & Spirits Research Center, Mercian Corporation, Fujisawa, Japan.
Suzuki
Y
Y
Okuda
T
T
Yokotsuka
K
K
D002851:Chromatography, High Pressure Liquid; D005960:Glucosides / Q000032:analysis*; D007536:Isomerism; D007564:Japan; D015203:Reproducibility of Results; D000077185:Resveratrol; D019685:Rosales; D013267:Stilbenes / Q000032:analysis*; D014920:Wine / Q000032:analysis*
D005960:Glucosides; D013267:Stilbenes; D000077185:Resveratrol; C058229:polydatin