Impairment of bone and mineral metabolism after pancreatic transplantation: observations in syngeneic rats.
OBJECTIVE
To find out whether transplantation of the duodenum and pancreas results in osteopathy in immunotolerant recipient rats.


METHODS
Prospective study.


METHODS
University hospital, Germany.


METHODS
30 male Lewis rats weighing about 315 g.


METHODS
Laparotomy alone (n = 10, control), or transplantation by one of three techniques: ectopic extraportal (EE, n = 5), ectopic intraportal (EI, n = 7), or orthotopic intraportal (OI, n = 8).


METHODS
Body weight; changes in mineral and bone metabolism, bone density and ash, and the breaking force of bone.


RESULTS
Rats were followed for 133 plus or minus 3 days. Compared with the control group the rats that had undergone transplantation had delayed gain in body weight and the final weight was reduced. Urinary glucose and basal serum glucose and insulin concentrations were unaffected, but that of pancreatic polypeptide was low. In the transplanted groups intestinal absorption of calcium and phosphorus and serum concentrations of calcium and parathyroid hormone were within reference ranges. Serum magnesium (EI and OI) and phosphorus (EI) concentrations, and the urinary calcium (FE) and phosphorus (EI) concentrations, and the urinary hydroxyproline:serum osteocalcin ratio (FE, and EI) were significantly higher than in controls (p < 0.01).


CONCLUSIONS
Transplantation failed to disturb glucometabolic functions and intestinal mineral absorption but was associated with an osteopathy which showed itself in high bone resorption and reduced bone phosphorus and magnesium concentrations. The osteopathy developed irrespective of the technique of reconstruction of the digestive tract and so may be caused by division of intestinal nerves or their incomplete regeneration.
Cesnjevar
R
R
Division of Experimental Surgery, University of Erlangen, Germany.
Schwille
P O
PO
Kissler
H
H
Berge
B
B
Köckerling
F
F
Fries
W
W
Hohenberger
W
W
D000818:Animals; D001835:Body Weight; D001842:Bone and Bones / Q000378:metabolism*; D006706:Homeostasis; D007413:Intestinal Mucosa / Q000378:metabolism; D008297:Male; D008903:Minerals / Q000378:metabolism*; D016035:Pancreas Transplantation / Q000502:physiology*; D011446:Prospective Studies; D051381:Rats; D011917:Rats, Inbred Lew
D008903:Minerals