Immunolocalization of vacuolar-type H+-ATPase in rat submandibular gland and adaptive changes induced by acid-base disturbances.
Using antibodies against the 31-kD and 70-kD subunits of vacuolar type H+-ATPase (V-ATPase) and light microscopic immunocytochemistry, we have demonstrated the presence of this V-ATPase in rat submandibular gland. We have also investigated the adaptive changes of this transporter during acid-base disturbances such as acute and chronic metabolic acidosis or alkalosis. Our results show intracellularly distributed V-ATPase in striated, granular, and main excretory duct cells in controls, but no V-ATPase immunoreaction in acinar cells. Both acute and chronic metabolic acidosis caused a shift in V-ATPase away from diffuse distribution towards apical localization in striated and granular duct cells, suggesting that a V-ATPase could be involved in the regulation of acid-base homeostasis. In contrast, during acidosis the main excretory duct cells showed no changes in the V-ATPase distribution compared to controls. With acute and chronic metabolic alkalosis, no changes in the V-ATPase distribution occurred. (J Histochem Cytochem 46:91-100, 1998)
Roussa
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E
Anatomisches Institut, Medizinische Fakultät, Universität des Saarlandes, Homburg/Saar, Germany.
Thévenod
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F
Sabolic
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Herak-Kramberger
C M
CM
Nastainczyk
W
W
Bock
R
R
Schulz
I
I
D000137:Acid-Base Imbalance / Q000139:chemically induced; D000138:Acidosis / Q000139:chemically induced / Q000201:enzymology; D000222:Adaptation, Physiological / Q000187:drug effects; D000471:Alkalosis / Q000139:chemically induced / Q000201:enzymology; D000643:Ammonium Chloride / Q000494:pharmacology; D000818:Animals; D015153:Blotting, Western; D007150:Immunohistochemistry; D008297:Male; D006180:Proton-Translocating ATPases / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar; D018987:Salivary Ducts / Q000166:cytology / Q000187:drug effects / Q000201:enzymology; D017693:Sodium Bicarbonate / Q000494:pharmacology; D013363:Submandibular Gland / Q000166:cytology / Q000187:drug effects / Q000201:enzymology*; D025262:Vacuolar Proton-Translocating ATPases
D000643:Ammonium Chloride; D017693:Sodium Bicarbonate; D025262:Vacuolar Proton-Translocating ATPases; D006180:Proton-Translocating ATPases