title: The fetoplacental pressor effects of low-dose acetylsalicylic acid and angiotensin II in the ex vivo cotyledon model.
abstract: OBJECTIVE
  Our purpose was to investigate perfusion pressure changes ex vivo induced by angiotensin II on fetoplacental vasculature pretreated with low-dose acetylsalicylic acid.
  
  
  METHODS
  Two cotyledons from each of 12 placentas were perfused. The intervillous space of one cotyledon was infused with acetylsalicylic acid (5 x 10(-5) mol/L) similar to the serum concentration of women receiving daily low-dose aspirin therapy (60 to 81 mg). The control cotyledon was infused with an equivalent amount of normal saline solution. Two doses of angiotensin II, 1 x 10(-11.5) and 1 x 10(-10) moles, were injected as boluses into the chorionic arteries of each cotyledon. A 3 x 10(-7) mole dose of angiotensin II was also injected into the intervillous space. Statistical analysis was performed with analysis of variance, and results are expressed as mean pressure change in millimeters of mercury +/- SEM.
  
  
  RESULTS
  Perfusion pressure response did not vary between cotyledons pretreated with acetylsalicylic acid and control cotyledons when 3 x 10(-7) moles of angiotensin II was injected into the intervillous space (8.0 +/- 1.9 mm Hg vs 9.8 +/- 1.6 mm Hg, p = 0.59). There were no differences between cotyledons in pressure response to 1 x 10(-11.5) moles of angiotensin II injected into the fetal circuit (5.9 +/- 0.8 mm Hg vs 6.7 +/- 0.9 mm Hg, p = 0.51). However, in the cotyledons pretreated with acetylsalicylic acid there was a decrease in the pressor response to 1 x 10(-10) moles of angiotensin II (14.1 +/- 1.4 mm Hg vs 21.5 +/- 3.3 mm Hg, p = 0.05).
  
  
  CONCLUSIONS
  Low-dose aspirin infused into the intervillous space decreases vasoconstriction elicited by angiotensin II in the fetoplacental compartment. This suggests that maternal low-dose aspirin therapy has effects in the fetoplacental circulation in addition to its effects in the maternal circulation.
authors.affiliation: Division of Maternal-Fetal Medicine, Madigan Army Medical Center, Fort Lewis, Washington, USA.
authors.forename: P G
authors.initials: PG
authors.lastname: Napolitano
authors.forename: N J
authors.initials: NJ
authors.lastname: Hoeldtke
authors.forename: K H
authors.initials: KH
authors.lastname: Moore
authors.forename: B C
authors.initials: BC
authors.lastname: Calhoun
authors.forename: E D
authors.initials: ED
authors.lastname: Christensen
authors.forename: G R
authors.initials: GR
authors.lastname: Markenson
authors.forename: R F
authors.initials: RF
authors.lastname: Hume
mesh_terms: D000804:Angiotensin II / Q000494:pharmacology*; D001241:Aspirin / Q000494:pharmacology*; D001794:Blood Pressure / Q000187:drug effects*; D005260:Female; D005333:Fetus / Q000098:blood supply*; D006801:Humans; D010477:Perfusion; D010920:Placenta / Q000098:blood supply*; D011247:Pregnancy
chemical_list: D000804:Angiotensin II; D001241:Aspirin