title: Flow cytometric analysis of Thy-1 expression in CD34-positive acute leukemia.
abstract: We analyzed the expression of the Thy-1 antigen (CD90) in CD34+ acute leukemia using two-color flow cytometry. Leukemic cells were obtained from the bone marrow (BM) and/or the peripheral blood (PB) of 57 patients: 37 with acute myelogenous leukemia (AML) including nine with secondary AML following myelodysplastic syndrome (MDS/AML), and 20 with acute lymphoblastic leukemia (ALL) including three with chronic myelogenous leukemia in blast crisis (CML-BC) of the lymphoid type. Among these patients, one (3.6%) with de novo AML, two (22.2%) with MDS/AML, three (17.6%) with de novo ALL, and two (66.7%) with CML-BC coexpressed CD34 and Thy-1 (CD34+ Thy-1+) on more than 20% of the mononuclear cells within 'lymph' plus 'blast' window. Thy-1 was rarely expressed in de novo acute leukemia especially in AML. Interestingly, in 1 patient with CML-BC, the leukemic cells in BM were divided into two subpopulations (CD34+ Thy-1low and CD34+ Thy-1high), whereas most of the CD34+ leukemic cells in PB were Thy-1high. However, the mechanism for the mobilization of CD34+ Thy-1high leukemic cells into the PB is unknown.
authors.affiliation: Department of Clinical Laboratory and Medicine, Kyoto Prefectural University of Medicine, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Inaba
authors.forename: C
authors.initials: C
authors.lastname: Shimazaki
authors.forename: T
authors.initials: T
authors.lastname: Sumikuma
authors.forename: R
authors.initials: R
authors.lastname: Takahashi
authors.forename: H
authors.initials: H
authors.lastname: Hirai
authors.forename: T
authors.initials: T
authors.lastname: Kikuta
authors.forename: Y
authors.initials: Y
authors.lastname: Sudo
authors.forename: N
authors.initials: N
authors.lastname: Yamagata
authors.forename: E
authors.initials: E
authors.lastname: Ashihara
authors.forename: H
authors.initials: H
authors.lastname: Goto
authors.forename: N
authors.initials: N
authors.lastname: Fujita
authors.forename: M
authors.initials: M
authors.lastname: Yoshimura
authors.forename: M
authors.initials: M
authors.lastname: Nakagawa
mesh_terms: D000208:Acute Disease; D018952:Antigens, CD34 / Q000097:blood*; D005434:Flow Cytometry; D006801:Humans; D007938:Leukemia / Q000276:immunology*; D018800:Thy-1 Antigens / Q000032:analysis*
chemical_list: D018952:Antigens, CD34; D018800:Thy-1 Antigens