title: Mechanism of apoptosis induced by cisplatin and VP-16 in PANC-1 cells.
abstract: Cisplatin and VP-16 were used to study the induction of apoptosis in Panc-1 cells. Cisplatin and VP-16 inhibited the growth of Panc-1 cells. After 2 hours exposure to cisplatin or VP-16, attached and detached cells were subjected to TUNEL staining to calculate the ratio of apoptosis. In detached cells TUNEL positive ratios increased in a time- and dose-dependent manner. In Western blotting, Bax expression was obviously up-regulated, but Bcl-2 remained almost constant. The results suggested that in Panc-1 cells cisplatin and VP-16 induced apoptotic cell death which was mediated through the interaction of Bax expression in the presence of mutated p53.
authors.affiliation: First Department of Surgery, Kyoto University, Japan.
authors.forename: J U
authors.initials: JU
authors.lastname: Lee
authors.forename: R
authors.initials: R
authors.lastname: Hosotani
authors.forename: M
authors.initials: M
authors.lastname: Wada
authors.forename: R
authors.initials: R
authors.lastname: Doi
authors.forename: T
authors.initials: T
authors.lastname: Kosiba
authors.forename: K
authors.initials: K
authors.lastname: Fujimoto
authors.forename: Y
authors.initials: Y
authors.lastname: Miyamoto
authors.forename: C
authors.initials: C
authors.lastname: Mori
authors.forename: N
authors.initials: N
authors.lastname: Nakamura
authors.forename: K
authors.initials: K
authors.lastname: Shiota
authors.forename: M
authors.initials: M
authors.lastname: Imamura
mesh_terms: D000970:Antineoplastic Agents / Q000494:pharmacology; D017209:Apoptosis / Q000187:drug effects*; D002448:Cell Adhesion / Q000187:drug effects; D002945:Cisplatin / Q000494:pharmacology*; D053938:DNA Fragmentation; D004305:Dose-Response Relationship, Drug; D005047:Etoposide / Q000494:pharmacology*; D016158:Genes, p53; D006131:Growth Inhibitors / Q000494:pharmacology; D006801:Humans; D010190:Pancreatic Neoplasms; D011518:Proto-Oncogene Proteins / Q000378:metabolism; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000378:metabolism; D014407:Tumor Cells, Cultured; D051028:bcl-2-Associated X Protein
chemical_list: D000970:Antineoplastic Agents; C494826:BAX protein, human; D006131:Growth Inhibitors; D011518:Proto-Oncogene Proteins; D019253:Proto-Oncogene Proteins c-bcl-2; D051028:bcl-2-Associated X Protein; D005047:Etoposide; D002945:Cisplatin