Interleukin-3 activates Syk in a human myeloblastic leukemia cell line, AML193.
Protein-tyrosine kinases and phosphatases play an important role in cytokine-mediated cell growth. The proliferation of a human myeloid leukemia cell line, AML193, is dependent on interleukin-3 (IL-3) or granulocyte colony-stimulating factor. In the current study, we demonstrated that a non-receptor-type protein-tyrosine kinase, Syk, was immediately activated by the stimulation with IL-3 or granulocyte colony-stimulating factor in AML193 cells. We further investigated the relation of Syk with IL-3-mediated signaling and found that the IL-3 receptor beta subunit was immunoprecipitated with Syk. Since the IL-3 receptor beta subunit is known to mediate growth signaling, our results indicate that Syk may be involved in the proliferation of myeloid cells.
Tsubokawa
M
M
Department of Otorhinolaryngology, Fukui Medical School, Matsuoka, Japan.
Tohyama
Y
Y
Tohyama
K
K
Asahi
M
M
Inazu
T
T
Nakamura
H
H
Saito
H
H
Yamamura
H
H
D015153:Blotting, Western; D004789:Enzyme Activation; D004792:Enzyme Precursors / Q000276:immunology / Q000378:metabolism*; D016179:Granulocyte Colony-Stimulating Factor / Q000494:pharmacology; D006801:Humans; D007377:Interleukin-3 / Q000276:immunology / Q000494:pharmacology*; D047908:Intracellular Signaling Peptides and Proteins; D015470:Leukemia, Myeloid, Acute / Q000201:enzymology*; D010766:Phosphorylation; D019000:Phosphotyrosine / Q000378:metabolism; D011233:Precipitin Tests; D011505:Protein-Tyrosine Kinases / Q000276:immunology / Q000378:metabolism*; D016185:Receptors, Interleukin-3 / Q000378:metabolism; D015398:Signal Transduction / Q000502:physiology; D000072377:Syk Kinase; D014407:Tumor Cells, Cultured
D004792:Enzyme Precursors; D007377:Interleukin-3; D047908:Intracellular Signaling Peptides and Proteins; D016185:Receptors, Interleukin-3; D016179:Granulocyte Colony-Stimulating Factor; D019000:Phosphotyrosine; D011505:Protein-Tyrosine Kinases; C000606896:SYK protein, human; D000072377:Syk Kinase