title: Determination of trace boron in microsamples of biological tissues.
abstract: A benign-by-design method for the determination of boron (B) in microsamples of biological tissues was developed. This is a simple, automated, microdigestion method. Use of reagents and generation of waste are minimized, and the use of toxic/hazardous reagents is eliminated as compared to currently available B methodology. Microsamples are accommodated by the method; 100-400 mg samples were used in this study. B is determined by inductively coupled plasma atomic emission spectrometry (ICPAES) at 249.678 nm. The instrument detection limit for B is 0.01 microgram/mL. Interference studies have been investigated for 21 common elements. Over 250 analyses of standard reference materials were analyzed during the study duration. Recoveries for a series of biological tissues, both plant and animal, ranged from 82-104%.
authors.affiliation: University of Idaho, Department of Food Science and Toxicology, Moscow 83844-2201, USA.
authors.forename: K A
authors.initials: KA
authors.lastname: Anderson
authors.forename: M L
authors.initials: ML
authors.lastname: Tschirgi
mesh_terms: D000818:Animals; D001895:Boron / Q000032:analysis* / Q000097:blood; D001921:Brain / Q000378:metabolism; D002417:Cattle; D007668:Kidney / Q000378:metabolism; D008099:Liver / Q000378:metabolism; D051379:Mice; D008832:Microchemistry; D010944:Plants / Q000378:metabolism; D012015:Reference Standards; D015203:Reproducibility of Results; D012867:Skin / Q000378:metabolism; D013052:Spectrometry, X-Ray Emission / Q000379:methods; D014867:Water / Q000032:analysis
chemical_list: D014867:Water; D001895:Boron