Determination of SDZ ICM 567 in blood and muscle microdialysis samples by microbore liquid chromatography with ultraviolet and fluorescence detection.
Fast, simple and accurate methods for the determination of SDZ ICM 567, the 7-methoxy derivative of tropisetron, in microdialysates have been developed. Sampling by microdialysis from freely moving rats in the portal and jugular vein offers a new technology for pharmacokinetic studies by direct and continuous measurement of unbound drug concentrations with time. SDZ ICM 567 can be identified in small sample volumes of dialysates on a microbore high-performance liquid chromatography column-switching system with ultraviolet detection. In addition, determination of SDZ ICM 567 by fluorimetric detection has been developed for muscle microdialysates from rats. [14C]SDZ ICM 567 was used as reference substance for the estimation of the amount of substance transferred through the dialysis membrane. The radioactive measurement (RA) gave the recovery information, whereas the liquid chromatographic method detected the sum of [14C]SDZ ICM 567 and dialyzed SDZ ICM 567.
Ofner
B
B
Institute of Pharmaceutical Chemistry, Graz, Austria.
Boukhabza
A
A
Pacha
W
W
Amsterdam
C V
CV
Wintersteiger
R
R
D000284:Administration, Oral; D000818:Animals; D014151:Anti-Anxiety Agents / Q000008:administration & dosage / Q000032:analysis / Q000097:blood* / Q000493:pharmacokinetics; D019086:Bridged Bicyclo Compounds, Heterocyclic / Q000008:administration & dosage / Q000032:analysis / Q000097:blood* / Q000493:pharmacokinetics; D002853:Chromatography, Liquid; D015314:Dialysis Solutions / Q000032:analysis*; D007211:Indoles / Q000008:administration & dosage / Q000032:analysis / Q000097:blood* / Q000493:pharmacokinetics; D007275:Injections, Intravenous; D008297:Male; D017551:Microdialysis / Q000379:methods; D010369:Pectoralis Muscles / Q000737:chemistry*; D051381:Rats; D017208:Rats, Wistar; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013050:Spectrometry, Fluorescence; D013056:Spectrophotometry, Ultraviolet; D014680:Veins
D014151:Anti-Anxiety Agents; D019086:Bridged Bicyclo Compounds, Heterocyclic; D015314:Dialysis Solutions; D007211:Indoles; C094117:SDZ ICM 567