Urinary calcium excretion and renal calbindin-D28k.
The present investigation examined the possible influence of urinary calcium excretion on the concentration of renal calbindin-D28k. Thiazide diuretics stimulate calcium transport across the epithelial cells of the distal tubule, which express calbindin-D28k in high concentrations. Calbindin-D28k is assumed to facilitate transcellular Ca diffusion. Reduced urine calcium excretion and increased urine output were induced in Wistar rats by infusion of bendroflume-thiazide 1 mg/kg/day. The two control groups had infusions of either furosemide 20 mg/kg/day or vehicle, n = 8 in each group. Urinary Ca excretion was reduced to 10% in the thiazide group and increased by 50% in the furosemide group. Renal concentrations of calbindin-D28 showed no difference between vehicle, thiazide- and furosemide-treated rats. No differences in plasma concentrations of calcium, magnesium, phosphorus, urea, PTH, calcitonin and 1,25-(OH)2D were found between the groups. The present study describes that urine calcium excretion selectively can be manipulated without accompanying changes in renal calbindin-D28k concentrations. The data, therefore, suggest that urinary calcium excretion is not a significant determinator of cytosolic concentrations of renal calbindin-D28k.
Hemmingsen
C
C
Department of Nephrology, Institute of Experimental Pathology, Rigshospitalet, University of Copenhagen, Denmark.
Staun
M
M
Meibom
K
K
Bang
K
K
Olgaard
K
K
D000818:Animals; D001581:Benzothiadiazines; D064092:Calbindin 1; D064026:Calbindins; D002118:Calcium / Q000652:urine*; D003600:Cytosol / Q000378:metabolism*; D004232:Diuretics; D007668:Kidney / Q000378:metabolism*; D008297:Male; D051381:Rats; D017208:Rats, Wistar; D064030:S100 Calcium Binding Protein G / Q000378:metabolism*; D049993:Sodium Chloride Symporter Inhibitors / Q000494:pharmacology*
D001581:Benzothiadiazines; C576500:Calb1 protein, rat; D064092:Calbindin 1; D064026:Calbindins; D004232:Diuretics; D064030:S100 Calcium Binding Protein G; D049993:Sodium Chloride Symporter Inhibitors; D002118:Calcium