Biliary excretion of dolichols and beta-hexosaminidase--effect of ethanol and glucagon.
Alcohol has been reported to increase the urinary excretion of dolichols, and urinary dolichols are suggested to be derived from the lysosomes of the renal cells. In the present study we examined the effects of alcohol and glucagon on the biliary excretion of dolichols in rats. Chronic ethanol treatment decreased both biliary dolichol and beta-hexosaminidase excretion. The absolute amount of dolichol excreted into the bile correlated highly significantly with the absolute amount of biliary beta-hexosaminidase. Our results indicate that biliary dolichols are--at least in part--derived from hepatic lysosomes. Decreased biliary dolichol output during chronic alcohol administration suggests that urinary and biliary dolichol excretions are regulated independently of each other.
Humaloja
K
K
Research Unit of Alcohol Diseases, Helsinki University Central Hospital, Finland.
Salaspuro
M
M
Roine
R P
RP
D000818:Animals; D001646:Bile / Q000187:drug effects / Q000378:metabolism*; D004286:Dolichols / Q000378:metabolism* / Q000652:urine; D000431:Ethanol / Q000008:administration & dosage / Q000494:pharmacology*; D005934:Glucagon / Q000008:administration & dosage / Q000494:pharmacology*; D008099:Liver / Q000648:ultrastructure; D008247:Lysosomes / Q000378:metabolism; D008297:Male; D051381:Rats; D017208:Rats, Wistar; D001619:beta-N-Acetylhexosaminidases / Q000378:metabolism*
D004286:Dolichols; D000431:Ethanol; D005934:Glucagon; D001619:beta-N-Acetylhexosaminidases