title: Mismatch-sensitive hybridization detection by peptide nucleic acids immobilized on a quartz crystal microbalance.
abstract: A quartz crystal microbalance DNA hybridization biosensor, based on thiol-derivatized peptide nucleic acid (PNA) probes, offers unusual in situ differentiation of single-base mismatches. A large excess of a single-base mismatch oligonucleotide has no effect on the frequency response of the target. Such remarkable distinction between perfect matches and mismatches is illustrated by the detection of a common mutation in the p53 gene. The greater specificity of the new mass-sensitive indicatorless hybridization device over those of analogous PNA-based carbon electrodes is attributed to the formation of a PNA monolayer and the use of a hydrophilic ethylene glycol linker. The improved specificity is coupled to very fast (3-5 min) hybridization in a low-ionic-strength medium.
authors.affiliation: Department of Chemistry and Biochemistry, New Mexico State University, Las Cruces 88003, USA.
authors.forename: J
authors.initials: J
authors.lastname: Wang
authors.forename: P E
authors.initials: PE
authors.lastname: Nielsen
authors.forename: M
authors.initials: M
authors.lastname: Jiang
authors.forename: X
authors.initials: X
authors.lastname: Cai
authors.forename: J R
authors.initials: JR
authors.lastname: Fernandes
authors.forename: D H
authors.initials: DH
authors.lastname: Grant
authors.forename: M
authors.initials: M
authors.lastname: Ozsoz
authors.forename: A
authors.initials: A
authors.lastname: Beglieter
authors.forename: M
authors.initials: M
authors.lastname: Mowat
mesh_terms: D015374:Biosensing Techniques; D016158:Genes, p53; D009692:Nucleic Acid Heteroduplexes; D009693:Nucleic Acid Hybridization; D015345:Oligonucleotide Probes; D010455:Peptides
chemical_list: D009692:Nucleic Acid Heteroduplexes; D015345:Oligonucleotide Probes; D010455:Peptides