title: A new MHC locus that influences class I peptide presentation.
abstract: We have investigated the HLA-B27-restricted CTL response to HY minor histocompatibility antigens in rats and mice transgenic for HLA-B27 and human beta2-microglobulin. A polymorphism was found at a locus within the H2 complex, producing two distinct but overlapping sets of B27-presented HY peptides. The locus, named Cim2, mapped between the K and Pb loci, and its product is therefore distinct from TAP, LMP, and tapasin. Identical findings in rats and mice, including identical HY peptide sequences and the failure of a rat Tap2A transgene to alter CTL recognition, suggest that a homologous locus with similar polymorphism exists in the rat. Cim2, or a closely linked locus, was found to exert a broad effect on peptide loading of both HLA-B27 and mouse class I alleles. The data thus establish a strong, previously unrecognized MHC-encoded influence on the class I antigen pathway.
authors.affiliation: Harold C. Simmons Arthritis Research Center, Department of Internal Medicine, University of Texas Southwestern Medical Center, Dallas 75235, USA.
authors.forename: W A
authors.initials: WA
authors.lastname: Simmons
authors.forename: D C
authors.initials: DC
authors.lastname: Roopenian
authors.forename: S G
authors.initials: SG
authors.lastname: Summerfield
authors.forename: R C
authors.initials: RC
authors.lastname: Jones
authors.forename: B
authors.initials: B
authors.lastname: Galocha
authors.forename: G J
authors.initials: GJ
authors.lastname: Christianson
authors.forename: S D
authors.initials: SD
authors.lastname: Maika
authors.forename: M
authors.initials: M
authors.lastname: Zhou
authors.forename: S J
authors.initials: SJ
authors.lastname: Gaskell
authors.forename: R S
authors.initials: RS
authors.lastname: Bordoli
authors.forename: H L
authors.initials: HL
authors.lastname: Ploegh
authors.forename: C A
authors.initials: CA
authors.lastname: Slaughter
authors.forename: K F
authors.initials: KF
authors.lastname: Lindahl
authors.forename: R E
authors.initials: RE
authors.lastname: Hammer
authors.forename: J D
authors.initials: JD
authors.lastname: Taurog
mesh_terms: D000071450:ATP Binding Cassette Transporter, Subfamily B, Member 3; D018528:ATP-Binding Cassette Transporters / Q000502:physiology; D000818:Animals; D030801:Animals, Genetically Modified; D002874:Chromosome Mapping; D003602:Cytotoxicity, Immunologic; D006182:H-Y Antigen / Q000276:immunology; D015796:HLA-B27 Antigen / Q000276:immunology; D015395:Histocompatibility Antigens Class I / Q000276:immunology*; D006801:Humans; D007111:Immunity, Cellular; D008285:Major Histocompatibility Complex; D051379:Mice; D010455:Peptides / Q000276:immunology; D011110:Polymorphism, Genetic; D051381:Rats; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology*
chemical_list: D000071450:ATP Binding Cassette Transporter, Subfamily B, Member 3; D018528:ATP-Binding Cassette Transporters; D006182:H-Y Antigen; D015796:HLA-B27 Antigen; D015395:Histocompatibility Antigens Class I; D010455:Peptides; C489516:Tap2 protein, mouse; C489514:Tap2 protein, rat; C071692:TAP2 protein, human