Highly aligned lipid membrane systems in the physiologically relevant "excess water" condition.
The "excess water" condition in biologically relevant systems is met when a membrane mesophase coexists with excess bulk water. Further addition of water to such a system results in no change to any of the system's physical properties (e.g., transition temperature, repeat spacing, and structural mesophases). Moreover, because biological membranes are anisotropic systems, many of their properties are best studied using aligned samples. Although model membrane systems are routinely aligned, they have traditionally been hydrated with water vapor. It is well known that membranes exposed to water vapor at 100% humidity do not imbibe the same quantity of water as a sample in contact with liquid water. As such, membranes that have been hydrated with water vapor have physical properties different from those of membranes dispersed in water. Because of this shortcoming, aligned membranes have not been utilized to their full potential. Here we present a novel and simple method of aligning model membrane systems under conditions of excess water, which will make possible, for the first time, a variety of techniques (e.g., neutron and x-ray diffraction, nuclear magnetic resonance, electron spin resonance, attenuated total reflection infrared spectroscopy, etc.) for studying such systems under physiologically relevant conditions. In addition, when dealing with samples of limited availability, the system allows for the conditions (buffer pH and ionic strength) to be altered without any effect on the sample's alignment.
Katsaras
J
J
National Research Council, Steacie Institute for Molecular Sciences, Chalk River Laboratories, Ontario, Canada. john.katsaras@nrc.ca
D015060:1,2-Dipalmitoylphosphatidylcholine / Q000737:chemistry; D055592:Biophysical Phenomena; D001703:Biophysics; D066298:In Vitro Techniques; D008563:Membrane Lipids / Q000737:chemistry*; D009502:Neutrons; D010714:Phosphatidylethanolamines / Q000737:chemistry; D012542:Scattering, Radiation; D013816:Thermodynamics; D014867:Water / Q000737:chemistry; D014961:X-Ray Diffraction
D008563:Membrane Lipids; D010714:Phosphatidylethanolamines; D014867:Water; D015060:1,2-Dipalmitoylphosphatidylcholine; C007510:1,2-dipalmitoyl-3-phosphatidylethanolamine