title: Leukocytes modulate 11beta-hydroxysteroid dehydrogenase (11beta-HSD) activity in human granulosa-lutein cell cultures.
abstract: It is well established that there are interactions between the immune and reproductive systems. The ovary contains indigenous macrophages, as well as other classes of leukocytes in smaller numbers. Cytokines secreted by these cells have been shown to have the ability to regulate ovarian steroidogenesis. In the present study, the effect of leukocytes on 11beta-hydroxysteroid dehydrogenase (11beta-HSD) in human granulosa-lutein cells was examined. In addition, individual cytokines were also tested for their ability to regulate this enzyme. The follicular aspirates of patients undergoing IVF treatment were used as a source of granulosa cells. Cells isolated from these aspirates were found to contain between 15 and 60% leukocytes as assessed by flow cytometry (FACS). Leukocytes were removed from the sample preparations by the use of immunomagnetic beads coated with CD45 antibody, which recognises a surface antigen on all classes of leukocyte. Removal of leukocytes significantly decreased the 11beta-HSD activity in the granulosa cells, assayed after 3 days of culture, from 7.3 (2-20) to 3.5 (1-10) pmol cortisone formed/50000 cells/4 h (medians and ranges, n = 15). Addition of IL-5 and IL-6 significantly increased the 11beta-HSD activity in granulosa cell cultures both in the presence and absence of leukocytes. Addition of IL-4 and IFN-gamma increased 11beta-HSD activity only in the leukocyte-depleted granulosa cell cultures, whereas IL-2 had no effect on either of the cultures. The data suggests that leukocytes interact with the ovarian cells through cytokine secretion and/or cell-cell contact to increase the 11beta-HSD activity in human granulosa cells.
authors.affiliation: Department of Biochemistry and Molecular Biology, Royal Free Hospital School of Medicine, University of London, UK.
authors.forename: M
authors.initials: M
authors.lastname: Evagelatou
authors.forename: S L
authors.initials: SL
authors.lastname: Peterson
authors.forename: B A
authors.initials: BA
authors.lastname: Cooke
mesh_terms: D042842:11-beta-Hydroxysteroid Dehydrogenases; D002450:Cell Communication; D002470:Cell Survival; D002478:Cells, Cultured; D004247:DNA / Q000032:analysis; D005260:Female; D006107:Granulosa Cells / Q000201:enzymology*; D006801:Humans; D006913:Hydroxysteroid Dehydrogenases / Q000378:metabolism*; D018189:Immunomagnetic Separation; D007371:Interferon-gamma / Q000494:pharmacology; D007378:Interleukins / Q000494:pharmacology; D017493:Leukocyte Common Antigens / Q000032:analysis; D007962:Leukocytes / Q000502:physiology*; D008184:Luteal Cells / Q000201:enzymology; D006080:Ovarian Follicle / Q000276:immunology
chemical_list: D007378:Interleukins; D007371:Interferon-gamma; D004247:DNA; D006913:Hydroxysteroid Dehydrogenases; D042842:11-beta-Hydroxysteroid Dehydrogenases; D017493:Leukocyte Common Antigens