Determination of low levels of poly(ethylene glycol) 400 in plasma and urine by capillary gas chromatography-selected ion-monitoring mass spectrometry after solid-phase extraction.
A convenient and sensitive method for the quantitative determination of poly(ethylene glycol) 400 in plasma and urine with capillary gas chromatography-mass spectrometry has been developed. The sample preparation involves solid-phase extraction with subsequent derivatization with heptafluorobutyric anhydride, which proved to give the most stable derivative. The derivatization procedure was optimized using experimental design, and different solid-phase extraction columns were evaluated. The limit of quantitation was 1 micromol/l (0.4 microg/ml) for both plasma and urine.
Fakt
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C
Bioanalytical Chemistry, Astra Hässle AB, Mölndal, Sweden.
Ervik
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M
D001774:Blood Chemical Analysis; D005466:Fluorocarbons; D008401:Gas Chromatography-Mass Spectrometry; D007202:Indicators and Reagents; D008970:Molecular Weight; D011092:Polyethylene Glycols / Q000032:analysis* / Q000737:chemistry; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D014556:Urine / Q000737:chemistry
D005466:Fluorocarbons; D007202:Indicators and Reagents; C007924:heptafluorobutyric anhydride; D011092:Polyethylene Glycols