Functional analysis of the sensory motor pathway of resistance reflex in crayfish. II. Integration Of sensory inputs in motor neurons.
The in vitro preparation of the fifth thoracic ganglion of the crayfish was used to analyze the connections supporting the monosynaptic reflex responses recorded from the depressor motor neurons (Dep MNs). Dep MNs are directly connected by the release-sensitive afferents from a proprioceptor, the coxo-basipodite chordotonal organ (CBCO), which is released by upward movements of the leg. Sine-wave movements, applied to the CBCO strand from the most released position, allowed us to stimulate the greatest part of release-sensitive CBCO fibers. Systematic intracellular recordings from all Dep MNs performed in high divalent cation saline allowed us to determine the connections between CBCO afferents and their postsynaptic Dep MNs: it highlighted the sequential activation of the different Dep MNs involved in the monosynaptic reflex. The convergence of different sensory afferents onto a given Dep MN, and the divergence of a given sensory afferent onto several Dep MNs illustrates the complexity of the sensory-motor reflex loops involved in the control of locomotion and posture. Electrophysiological experiments and simulations were performed to analyze the mechanisms by which Dep MNs integrate the large amount of sensory input that they receive. Paired intracellular recording experiments demonstrated that postsynaptic response shapes characteristic of both phasic and phaso-tonic afferents could be induced by varying the presynaptic firing frequency, whatever the postsynaptic Dep MN. Compartment model simulations were used to analyze the role of the sensory-motor synapse characteristics in the summation properties of postsynaptic MN. They demonstrated the importance of the postsynaptic compartment geometry, because large postsynaptic compartments allowed to generate greater excitatory postsynaptic potential (EPSP) summations than small ones. The results presented show that velocity information is the most effective to elicit large compound EPSPs in MNs. We therefore suggest that the negative feedback reflex is mainly based on the detection of leg movements.
Le Ray
D
D
Laboratoire de Neurobiologie et Mouvements, Centre National de la Recherche Scientifique, Marseille Cedex 20, France.
Clarac
F
F
Cattaert
D
D
D000818:Animals; D003400:Astacoidea / Q000502:physiology*; D003198:Computer Simulation; D004558:Electric Stimulation; D019706:Excitatory Postsynaptic Potentials; D005260:Female; D017952:Ganglia, Invertebrate / Q000502:physiology; D008297:Male; D008564:Membrane Potentials / Q000502:physiology; D008959:Models, Neurological; D009046:Motor Neurons / Q000502:physiology; D009068:Movement / Q000502:physiology; D009434:Neural Pathways / Q000502:physiology; D009475:Neurons, Afferent / Q000502:physiology; D012026:Reflex, Stretch / Q000502:physiology; D009435:Synaptic Transmission / Q000502:physiology