[The membrane phospholipid peroxidation and Ca-dependent ATPase activity of the microsomal fractions isolated from rat renal tissue in thermal ischemia with and without alpha-tocopherol protection].
The author studied the effects of 30-min heat ischemia of rat kidneys on the level of malonic dialdehyde (MDA) and Ca-dependent ATPase activity of microsomal fraction isolated from the cortical substance in the presence and absence of antibiotic alameticine and ortovandate in the incubation medium and protective action on Ca-ATPase activity of rat pretreatment with alpha-tocopherol (TP). It has been demonstrated that thermal ischemia induces inhibition of Ca-ATPase activity of microsomes resistant to vanadate. Administration of TP reduced MDA level, enhanced Ca-ATPase microsomal activity in the presence of alameticine against inhibition of enzymic activity in the absence of alameticine. This indicates a rise in the true enzyme activity under decreasing membrane permeability in conditions of diminishing activity of lipid peroxidation in response to TP effects.
Golod
E A
EA
D000408:Alamethicin / Q000494:pharmacology; D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology; D000975:Antioxidants / Q000627:therapeutic use*; D000252:Calcium-Transporting ATPases / Q000187:drug effects / Q000378:metabolism*; D004791:Enzyme Inhibitors / Q000494:pharmacology; D006358:Hot Temperature / Q000009:adverse effects*; D007511:Ischemia / Q000188:drug therapy* / Q000378:metabolism*; D007672:Kidney Cortex / Q000098:blood supply* / Q000187:drug effects* / Q000378:metabolism; D015227:Lipid Peroxidation / Q000187:drug effects*; D008297:Male; D008563:Membrane Lipids / Q000378:metabolism*; D008861:Microsomes / Q000187:drug effects* / Q000378:metabolism; D010743:Phospholipids / Q000378:metabolism*; D051381:Rats; D013997:Time Factors; D014638:Vanadates / Q000494:pharmacology; D014810:Vitamin E / Q000627:therapeutic use*
D000900:Anti-Bacterial Agents; D000975:Antioxidants; D004791:Enzyme Inhibitors; D008563:Membrane Lipids; D010743:Phospholipids; D014810:Vitamin E; D000408:Alamethicin; D014638:Vanadates; D000252:Calcium-Transporting ATPases