Synthesis of asymmetrically labeled sucrose by a recombinant sucrose synthase.
About 80% of radioactivity was recovered in asymmetrically labeled sucrose from UDP-[14C]glucose or [14C]fructose with recombinant mung bean sucrose synthase expressed in Escherichia coli harboring pEB-01. This high recovery is due to the fact that the enzyme conserving the activity of sucrose synthase has a similar affinity for UDP-glucose and fructose to an intact enzyme from the mung bean, but a lower affinity for sucrose.
Nakai
T
T
Wood Research Institute, Kyoto University, Japan.
Tonouchi
N
N
Tsuchida
T
T
Mori
H
H
Sakai
F
F
Hayashi
T
T
D002250:Carbon Radioisotopes / Q000737:chemistry; D004926:Escherichia coli / Q000201:enzymology / Q000235:genetics; D005632:Fructose / Q000737:chemistry / Q000378:metabolism; D005964:Glucosyltransferases / Q000235:genetics / Q000378:metabolism*; D007553:Isotope Labeling / Q000379:methods; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism; D013395:Sucrose / Q000138:chemical synthesis* / Q000378:metabolism; D014532:Uridine Diphosphate Glucose / Q000737:chemistry / Q000378:metabolism
D002250:Carbon Radioisotopes; D011994:Recombinant Proteins; D005632:Fructose; D013395:Sucrose; D005964:Glucosyltransferases; C022699:sucrose synthase; D014532:Uridine Diphosphate Glucose