Nitric oxide mediates NMDA-evoked [3H]GABA release from chick retina cells.
The stimulation of NMDA receptor increased [3H]GABA release from preloaded cultured retina cells. This effect appears to be mediated by NO production, since addition of L-NA reduces NMDA-evoked [3H]GABA release. Spermine/NO complex, an NO donor, mimics the effect produced by NMDA. The addition of zaprinast, a phosphodiesterase inhibitor, as well as 8-Br-cGMP enhances the NMDA-evoked [3H]GABA release. These results agree with the existence in chick retina cells of NO/cGMP pathways and support a role for NO in NMDA-evoked events. The activation of this receptor complex through maturative stages of the retina together with the NO-mediated increase in GABA release may account for NMDA differentiative effect in culturing retina cells.
Ientile
R
R
Institute of Biochemical Sciences and Clinical Biochemistry, Faculty of Medicine, University of Messina, Italy.
Pedale
S
S
Picciurro
V
V
Macaione
V
V
Fabiano
C
C
Macaione
S
S
D015056:1-Methyl-3-isobutylxanthine / Q000494:pharmacology; D000818:Animals; D002454:Cell Differentiation; D002478:Cells, Cultured; D002642:Chick Embryo; D006152:Cyclic GMP / Q000031:analogs & derivatives / Q000378:metabolism / Q000494:pharmacology; D016291:Dizocilpine Maleate / Q000494:pharmacology; D007546:Isoquinolines / Q000494:pharmacology; D016202:N-Methylaspartate / Q000494:pharmacology*; D009569:Nitric Oxide / Q000494:pharmacology*; D019335:Nitroarginine / Q000494:pharmacology; D009589:Nitrogen Oxides; D011688:Purinones / Q000494:pharmacology; D016194:Receptors, N-Methyl-D-Aspartate / Q000187:drug effects / Q000502:physiology*; D012160:Retina / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D013096:Spermine / Q000031:analogs & derivatives / Q000494:pharmacology; D014316:Tritium; D005680:gamma-Aminobutyric Acid / Q000378:metabolism*
D007546:Isoquinolines; D009589:Nitrogen Oxides; D011688:Purinones; D016194:Receptors, N-Methyl-D-Aspartate; D014316:Tritium; C070935:LY 274614; C091861:spermine nitric oxide complex; D019335:Nitroarginine; D013096:Spermine; C016276:8-bromocyclic GMP; D009569:Nitric Oxide; D005680:gamma-Aminobutyric Acid; D016202:N-Methylaspartate; D016291:Dizocilpine Maleate; C011145:zaprinast; D006152:Cyclic GMP; D015056:1-Methyl-3-isobutylxanthine