Cellular thioredoxin reductase activity is regulated by selenium.
Selenium (Se) is an essential trace element and has been reported to decrease the incidence of some human cancers. We have investigated the effects of Se on thioredoxin reductase, a selenocysteine containing flavoenzyme, in HT-29 human colon cancer cells grown in serum-free medium. Sodium selenite and other Se containing compounds produced a time and concentration dependent increase in intracellular thioredoxin reductase activity and protein levels. Selenite was the most active of the Se compounds examined: 1 microM selenite produced a 28-fold increase in thioredoxin reductase activity by 1 day and 10 microM selenite over a 60-fold increase by 5 days. The activity of a related non-selenocysteine containing flavoenzyme glutathione reductase was not increased by selenite. Selenite, but not the other Se containing compounds inhibited cell growth at concentrations above 2 microM. The results show that Se can produce large increases in cell thioredoxin reductase activity.
Berggren
M
M
Arizona Cancer Center, University of Arizona Health Sciences Center, Tucson 85724-5024, USA.
Gallegos
A
A
Gasdaska
J
J
Powis
G
G
D015153:Blotting, Western; D002455:Cell Division / Q000187:drug effects; D003110:Colonic Neoplasms / Q000201:enzymology; D003545:Cysteine / Q000031:analogs & derivatives / Q000378:metabolism; D006801:Humans; D016566:Organoselenium Compounds / Q000378:metabolism; D012643:Selenium / Q000502:physiology*; D017279:Selenocysteine / Q000031:analogs & derivatives; D012645:Selenomethionine / Q000378:metabolism; D013880:Thioredoxin-Disulfide Reductase / Q000378:metabolism*; D014407:Tumor Cells, Cultured / Q000201:enzymology
D016566:Organoselenium Compounds; D017279:Selenocysteine; D012645:Selenomethionine; D013880:Thioredoxin-Disulfide Reductase; D012643:Selenium; D003545:Cysteine; C002979:selenomethylselenocysteine