title: Purification, characterization, and nucleotide sequence of an intracellular maltotriose-producing alpha-amylase from Streptococcus bovis 148.
abstract: An intracellular alpha-amylase from Streptococcus bovis 148 was purified and characterized. The enzyme was induced by maltose and soluble starch and produced about 80% maltotriose from soluble starch. Maltopentaose was hydrolyzed to maltotriose and maltose and maltohexaose was hydrolyzed mainly to maltotriose by the enzyme. Maltotetraose, maltotriose, and maltose were not hydrolyzed. This intracellular enzyme was considered to be a maltotriose-producing enzyme. The enzymatic characteristics and hydrolysis product from soluble starch were different from those of the extracellular raw-starch-hydrolyzing alpha-amylase of strain 148. The deduced amino acid sequence of the intracellular alpha-amylase was similar to the sequences of the mature forms of extracellular liquefying alpha-amylases from Bacillus strains, although the intracellular alpha-amylase did not contain a signal peptide. No homology between the intracellular and extracellular alpha-amylases of S. bovis 148 was observed.
authors.affiliation: Department of Agricultural Chemistry, Tokyo University of Agriculture, Japan. satoh@voeding.tno.nl
authors.forename: E
authors.initials: E
authors.lastname: Satoh
authors.forename: T
authors.initials: T
authors.lastname: Uchimura
authors.forename: T
authors.initials: T
authors.lastname: Kudo
authors.forename: K
authors.initials: K
authors.lastname: Komagata
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D004269:DNA, Bacterial / Q000235:genetics; D005110:Extracellular Space / Q000201:enzymology; D005798:Genes, Bacterial; D007424:Intracellular Fluid / Q000201:enzymology; D008969:Molecular Sequence Data; D017386:Sequence Homology, Amino Acid; D013291:Streptococcus / Q000201:enzymology / Q000235:genetics; D016985:Streptococcus bovis / Q000201:enzymology* / Q000235:genetics*; D014312:Trisaccharides / Q000096:biosynthesis*; D000516:alpha-Amylases / Q000235:genetics* / Q000302:isolation & purification* / Q000378:metabolism
chemical_list: D004269:DNA, Bacterial; D014312:Trisaccharides; C008317:maltotriose; D000516:alpha-Amylases