title: Fast isolation of RNA to detect expression of tumor markers.
abstract: The expression status of several tumor-related proteins is of great interest in clinical examination and research. As a completion to conventional antibody staining, RT-PCR is often used today. Reliable isolation of RNA from a low number of cells is very often a critical stage of such an examination. We demonstrate here a simple and fast method to isolate RNA from only 10,000 cells and applied it to the detection of CEA, c-ERB-B2, and mdr-1 as often studied models for tumor markers.
authors.affiliation: Max Delbrück Center for Molecular Medicine (MDC) Berlin-Buch, Berlin, Germany.
authors.forename: J A
authors.initials: JA
authors.lastname: Schenk
authors.forename: T
authors.initials: T
authors.lastname: Hillebrand
authors.forename: L
authors.initials: L
authors.lastname: Lübbe
authors.forename: S
authors.initials: S
authors.lastname: Heymann
authors.forename: M
authors.initials: M
authors.lastname: Böttger
authors.forename: B
authors.initials: B
authors.lastname: Micheel
authors.forename: P
authors.initials: P
authors.lastname: Bendzko
mesh_terms: D020168:ATP Binding Cassette Transporter, Subfamily B, Member 1 / Q000032:analysis / Q000235:genetics; D014408:Biomarkers, Tumor / Q000032:analysis*; D002272:Carcinoembryonic Antigen / Q000032:analysis / Q000235:genetics; D015870:Gene Expression; D006801:Humans; D012334:RNA, Neoplasm / Q000302:isolation & purification*; D012194:RNA-Directed DNA Polymerase; D018719:Erb-b2 Receptor Tyrosine Kinases / Q000032:analysis / Q000235:genetics; D014407:Tumor Cells, Cultured
chemical_list: D020168:ATP Binding Cassette Transporter, Subfamily B, Member 1; D014408:Biomarkers, Tumor; D002272:Carcinoembryonic Antigen; D012334:RNA, Neoplasm; D012194:RNA-Directed DNA Polymerase; D018719:Erb-b2 Receptor Tyrosine Kinases