Protein degradation in red cells exposed to 2,2'-azo-bis(2-amidinopropane) derived radicals.
Extensive proteolysis is observed when red blood cells are exposed to free radicals produced in the thermolysis of 2,2'-azo-bis(2-amidinopropane). It is evaluated that nearly one amino terminal group is produced by each free radical introduced into the system. These groups are considered to arise mainly from band 3 fragmentation due to the action of red cell proteinases. Protein fragmentation takes place prior to significant hemolysis or lipid peroxidation, as evaluated by thiobarbituric acid-reactive substances measurements.
Celedón
G
G
Departamento de Fisiología, Facultad de Ciencias, Universidad de Valparaiso, Chile.
Lips
V
V
Alvarado
C
C
Cortés
M
M
Lissi
E A
EA
González
G
G
D000578:Amidines / Q000494:pharmacology*; D001798:Blood Proteins / Q000187:drug effects* / Q000378:metabolism*; D010450:Endopeptidases / Q000097:blood; D004910:Erythrocyte Membrane / Q000187:drug effects / Q000201:enzymology; D004912:Erythrocytes / Q000187:drug effects* / Q000201:enzymology / Q000378:metabolism*; D005609:Free Radicals / Q000494:pharmacology; D006461:Hemolysis / Q000187:drug effects; D006801:Humans; D006868:Hydrolysis / Q000187:drug effects; D015227:Lipid Peroxidation / Q000187:drug effects; D016877:Oxidants / Q000494:pharmacology; D010545:Peroxides / Q000494:pharmacology
D000578:Amidines; D001798:Blood Proteins; D005609:Free Radicals; D016877:Oxidants; D010545:Peroxides; C049375:perhydroxyl radical; C046728:2,2'-azobis(2-amidinopropane); D010450:Endopeptidases