Histamine and eicosanoid levels in plasma and peripheral blood leucocyte cultures during experimental infection of cattle with bluetongue virus serotype 11.
Three calves were sensitized with three doses of inactivated BTV-11 UC8 strain and then experimentally infected with the homologous virus. In addition, four BTV-seronegative heifers were also experimentally infected with BTV-11. Granulocyte rich fractions of peripheral blood leucocyte (PBL-GRF) cultures from BTV-sensitized/infected calves and from control unexposed cattle were exposed in vitro with BTV-11. Histamine, leukotriene (LT) C4 and prostaglandin (PG) D2 were assayed in supernatant fluids. Plasma histamine levels increased in BTV-infected heifers from 10.1 +/- 2 ng/ml at Day 0 to 23.1 +/- 6.6 ng/ml at Day 12 following virus exposure. In addition, in this experimental group the concentration of PGF2 alpha (mean 551.97 +/- 243.54 pg/ml) increased significantly (P < or = 0.05) compared with control cattle (mean 467.3 +/- 73.9 pg/ml). Bluetongue virus induced histamine and LTC4 release after in vitro infection of PBL-GRF. Release of LTC4 was significantly (P < or = 0.05) higher in PBL-GRF cultures from sensitized and control animals than in unexposed PBL-GRF cultures. In contrast to these results, PGD2 was not released after BTV infection of PBL-GRF in vitro. The histamine release caused by BTV was virus-specific and mainly mediated by an immunological reaction, since the release was significantly reduced by removal of cell surface immunoglobulins.
Odeón
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AC
Department of Pathology, Microbiology and Immunology, School of Veterinary Medicine, University of California, Davis, USA.
Giri
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SN
de la Concha-Bermejillo
A
A
Osburn
B I
BI
D000818:Animals; D001819:Bluetongue / Q000097:blood* / Q000378:metabolism / Q000473:pathology; D001820:Bluetongue virus / Q000145:classification / Q000302:isolation & purification / Q000502:physiology; D002417:Cattle; D002418:Cattle Diseases / Q000097:blood* / Q000378:metabolism / Q000473:pathology; D002478:Cells, Cultured; D015777:Eicosanoids / Q000097:blood* / Q000378:metabolism; D005260:Female; D006632:Histamine / Q000097:blood* / Q000378:metabolism; D007962:Leukocytes / Q000166:cytology / Q000378:metabolism* / Q000821:virology
D015777:Eicosanoids; D006632:Histamine