Antibody to human MHC class I inhibits SIVsmmPBj1.9-induced proliferation of pigtailed macaque lymphocytes.
Previously we have shown that the simian immunodeficiency virus SIVsmmPBj1.9, a molecular clone of SIVsmmPBj14, induces proliferation of human peripheral blood mononuclear cells (PBMC). We have extended this observation to show that SIVsmmPBj1.9 induces proliferation of PBMC from pigtailed macaques. This proliferative response was markedly inhibited by mAbs against human class I MHC, class II MHC and CD4 antigens, and partially inhibited by mAbs against integrin beta 2 subunit (CD18) and LFA-1 (CD11a). However, these antibodies differed in their ability to inhibit in vitro viral infectivity of PBMC. While anti-CD4, MHC class II, and LFA-1 strongly inhibited viral infectivity, antibodies to MHC class I demonstrated little effect on viral infectivity. A control antibody (PLM2) against porcine CD18 inhibited neither virus-induced proliferation nor viral infectivity. Based on these results, we suggest that SIVsmmPBj1.9-induced proliferation requires the participation of class I MHC, class II MHC and CD4 molecules. In addition, the observation that anti-class I MHC Ab inhibited proliferation of macaque PBMC induced by mitogen (PHA) and bacterial superantigens, such as Staphylococcus enterotoxin A and toxin shock syndrome toxin-1, suggests that SIVsmmPBj1.9 also contains a viral superantigen similar to that previously demonstrated in SIVsmmPBj14.
Baskar
P V
PV
Gerontology Research Center, NIA, NIH, Baltimore, Md., USA. baskarp@vax.grc.nia.nih.gov
Nagel
J E
JE
Narayan
O
O
McClure
H M
HM
Adler
W H
WH
Hildreth
J E
JE
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D001427:Bacterial Toxins; D002455:Cell Division; D004768:Enterotoxins / Q000494:pharmacology; D015395:Histocompatibility Antigens Class I / Q000276:immunology*; D006801:Humans; D007963:Leukocytes, Mononuclear / Q000166:cytology / Q000187:drug effects; D008214:Lymphocytes / Q000276:immunology*; D008251:Macaca; D008934:Mitogens / Q000494:pharmacology; D010835:Phytohemagglutinins / Q000494:pharmacology; D015302:Simian Immunodeficiency Virus / Q000378:metabolism / Q000502:physiology*; D018089:Superantigens / Q000494:pharmacology
D000911:Antibodies, Monoclonal; D001427:Bacterial Toxins; D004768:Enterotoxins; D015395:Histocompatibility Antigens Class I; D008934:Mitogens; D010835:Phytohemagglutinins; D018089:Superantigens; C049463:enterotoxin E, Staphylococcal; C030107:enterotoxin F, Staphylococcal; C026066:enterotoxin A, Staphylococcal