title: Topical application of 5-HT antibodies reduces edema and cell changes following trauma of the rat spinal cord.
abstract: Involvement of serotonin in the early microvascular reactions and cell changes following trauma of the spinal cord was examined using topical application of serotonin antibodies to the traumatised cord in a rat model. A focal trauma of the spinal cord was produced by making an incision into the right dorsal horn of the T10-11 segments (about 2 mm deep and 5 mm long); the animals were allowed to survive 5 h after injury. Monoclonal 5-HT antibodies (1:20) were applied (25 microliters in 10 sec) to the traumatised spinal cord 2 min after injury. There was a significant reduction in the breakdown of the blood-spinal cord barrier, edema formation, and cell changes in the traumatised rats which received 5-HT antiserum compared to the injured rats given saline. These results show that 5-HT is an important mediator involved in the early pathophysiological responses of spinal cord injury.
authors.affiliation: Department of Anatomy, Uppsala University, Sweden.
authors.forename: H S
authors.initials: HS
authors.lastname: Sharma
authors.forename: J
authors.initials: J
authors.lastname: Westman
authors.forename: F
authors.initials: F
authors.lastname: Nyberg
mesh_terms: D000287:Administration, Topical; D000818:Animals; D000911:Antibodies, Monoclonal / Q000627:therapeutic use*; D002199:Capillary Permeability; D004487:Edema / Q000628:therapy; D008297:Male; D051381:Rats; D017208:Rats, Wistar; D012701:Serotonin / Q000276:immunology*; D013116:Spinal Cord / Q000098:blood supply / Q000473:pathology; D013119:Spinal Cord Injuries / Q000473:pathology / Q000628:therapy*
chemical_list: D000911:Antibodies, Monoclonal; D012701:Serotonin