title: Platelet-derived growth factor activates mitogen-activated protein kinase in isolated caveolae.
abstract: The ability of a peptide hormone to affect many different intracellular targets is thought to be possible because of the modular organization of signal transducing molecules in the cell. Evidence for the presence of signaling modules in metazoan cells, however, is incomplete. Herein we show, with morphology and cell fractionation, that all the components of a mitogen-activated protein kinase pathway are concentrated in caveolae of unstimulated human fibroblasts. Addition of platelet-derived growth factor to either the intact cell or caveolae isolated from these cells stimulates tyrosine phosphorylation and activates mitogen-activated protein kinases in caveolae. The molecular machinery for kinase activation, therefore, is preorganized at the cell surface of quiescent cells.
authors.affiliation: Department of Cell Biology and Neuroscience, University of Texas Southwestern Medical Center, Dallas, TX 75235-9039, USA.
authors.forename: P
authors.initials: P
authors.lastname: Liu
authors.forename: Y
authors.initials: Y
authors.lastname: Ying
authors.forename: R G
authors.initials: RG
authors.lastname: Anderson
mesh_terms: D017871:Calcium-Calmodulin-Dependent Protein Kinases / Q000378:metabolism*; D002462:Cell Membrane / Q000187:drug effects* / Q000378:metabolism* / Q000648:ultrastructure; D002478:Cells, Cultured; D004789:Enzyme Activation / Q000187:drug effects; D006801:Humans; D066298:In Vitro Techniques; D008565:Membrane Proteins / Q000378:metabolism; D016253:Microscopy, Immunoelectron; D010750:Phosphoproteins / Q000378:metabolism; D010766:Phosphorylation; D010982:Platelet-Derived Growth Factor / Q000494:pharmacology*; D014443:Tyrosine / Q000378:metabolism
chemical_list: D008565:Membrane Proteins; D010750:Phosphoproteins; D010982:Platelet-Derived Growth Factor; D014443:Tyrosine; D017871:Calcium-Calmodulin-Dependent Protein Kinases