Modulation by interferon-gamma of zinc-alpha 2-glycoprotein gene expression in human epithelial cell lines.
Zinc-alpha 2-glycoprotein has been detected in most body fluids, and its antibody labels the corresponding glandular epithelia. We have also detected it in human stratified epithelia (epidermis and buccal mucosa). In this study, the mRNA levels of zinc-alpha 2-glycoprotein were found to be about twice as high in epithelial cells of mucosal origin (whether normal primaries or neoplastic cell lines) as in epidermoid cells (normal epidermal primary cultures, an immortalized but non-tumorigenic epidermal cell line, and neoplastic vulvar and cervical cell lines). Interferon-gamma strongly upregulated gene expression, but substantially less in mucosal than epidermoid cells. To compare responses as a clue to the function of zinc-alpha 2-glycoprotein, we ran parallel experiments with three markers of distinct properties, all known to be induced by interferon-gamma. There was the least resemblance for involucrin, a qualitative similarity for HLA-DR, and a rather better match for 2'-5' oligoadenylate synthetase.
Brysk
M M
MM
Department of Dermatology, University of Texas Medical Branch, Galveston 77555, USA.
Lei
G
G
Selvanayagam
P
P
Brysk
H
H
Tyring
S K
SK
Arany
I
I
D015088:2',5'-Oligoadenylate Synthetase / Q000378:metabolism; D002460:Cell Line; D004847:Epithelial Cells / Q000378:metabolism*; D005786:Gene Expression Regulation / Q000187:drug effects; D006023:Glycoproteins / Q000235:genetics*; D006684:HLA-DR Antigens / Q000378:metabolism; D006801:Humans; D007371:Interferon-gamma / Q000494:pharmacology*; D015603:Keratinocytes / Q000378:metabolism; D011498:Protein Precursors / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics; D029589:Seminal Plasma Proteins; D000090203:Zn-Alpha-2-Glycoprotein
D006023:Glycoproteins; D006684:HLA-DR Antigens; D011498:Protein Precursors; D012333:RNA, Messenger; D029589:Seminal Plasma Proteins; D000090203:Zn-Alpha-2-Glycoprotein; C032253:involucrin; D007371:Interferon-gamma; D015088:2',5'-Oligoadenylate Synthetase