Distribution of fibronectin, type I collagen, type IV collagen, and laminin in the cardiac jelly of the mouse embryonic heart with retinoic acid-induced complete transposition of the great arteries.
In the mouse model of complete transposition of the great arteries (TGA) produced by all-trans retinoic acid (RA), parietal and septal ridges in the outflow tract (OT) are hypoplastic. At first, these ridges are generated by an expanded cardiac jelly (mainly myocardial basement membrane). Thereafter, endothelial cells delaminate and invade into the adjacent cardiac jelly to form endocardial cushion tissue (formation of cushion ridge). During cushion tissue formation, basement membrane antigens play an important role in the regulation of this endothelial-mesenchymal transformation.



To examine whether the myocardial basement membrane components are altered in the RA-treated heart OT, immunohistochemistry for fibronectin, type I collagen, type IV collagen, and laminin was carried out in mouse embryonic hearts at 9.5 and 10.5 ED (embryonic day; vaginal plug = day 0) with or without prior exposure to RA.



Particulate/fibrillar fibronectin and fibrillar type I collagen were observed in the thick cardiac jelly of the control heart at the onset of mesenchymal formation. In the RA-treated heart, an intermittent patchy staining for fibronectin and a sparse distribution of type I collagen were observed in the thin cardiac jelly. Laminin and type IV collagen were distributed continuously on the basal surface (layer adjacent to the basal plasma membrane) of endocardium and myocardium in both control and RA-treated hearts.



The alterations in the antigens of the myocardial basement membrane (cardiac jelly) may be responsible for the hypoplasticity of parietal and septal ridges that characterizes RA-induced TGA morphology. This may be one of the reasons why mesenchymal cell formation is inhibited in the RA-induced TGA.
Nakajima
Y
Y
Department of Anatomy, Saitama Medical School, Japan.
Morishima
M
M
Nakazawa
M
M
Momma
K
K
Nakamura
H
H
D000818:Animals; D001485:Basement Membrane / Q000187:drug effects / Q000378:metabolism; D003094:Collagen / Q000378:metabolism*; D005109:Extracellular Matrix / Q000187:drug effects / Q000378:metabolism; D005260:Female; D005318:Fetal Heart / Q000002:abnormalities / Q000378:metabolism*; D005353:Fibronectins / Q000378:metabolism*; D006330:Heart Defects, Congenital / Q000139:chemically induced / Q000378:metabolism*; D007150:Immunohistochemistry; D007797:Laminin / Q000378:metabolism*; D008297:Male; D051379:Mice; D008813:Mice, Inbred ICR; D011247:Pregnancy; D014188:Transposition of Great Vessels / Q000139:chemically induced / Q000378:metabolism*; D014212:Tretinoin / Q000494:pharmacology*
D005353:Fibronectins; D007797:Laminin; D014212:Tretinoin; D003094:Collagen