eps15 and eps15R are essential components of the endocytic pathway.
eps15 and eps1SR are substrates of the epidermal growth factor (EGF) receptor kinase that are characterized by the presence of a protein:protein interaction domain, the EH domain, and by their ability to bind to the clathrin adaptor protein complex adaptor protein 2. Indirect evidence suggests that eps15 and eps15R are involved in endocytosis. Here we show that microinjection of antibodies against eps15 and eps15R inhibits internalization of EGF and transferrin. In addition, fragments of eps15 (encompassing its EH domains or the COOH-terminal region that binds to adaptor protein 2) inhibit EGF internalization or endocytosis of Sindbis virus. These results demonstrate that eps15 and eps15R are essential components of the endocytic machinery.
Carbone
R
R
Department of Experimental Oncology, European Institute of Oncology, Milan, Italy.
Fré
S
S
Iannolo
G
G
Belleudi
F
F
Mancini
P
P
Pelicci
P G
PG
Torrisi
M R
MR
Di Fiore
P P
PP
D016475:3T3 Cells / Q000378:metabolism / Q000502:physiology; D048868:Adaptor Proteins, Signal Transducing; D000818:Animals; D000906:Antibodies / Q000494:pharmacology; D019556:COS Cells / Q000378:metabolism / Q000502:physiology; D002135:Calcium-Binding Proteins / Q000276:immunology / Q000378:metabolism / Q000502:physiology*; D004705:Endocytosis / Q000502:physiology*; D066246:ErbB Receptors / Q000378:metabolism; D047908:Intracellular Signaling Peptides and Proteins; D051379:Mice; D008845:Microinjections; D010750:Phosphoproteins / Q000276:immunology / Q000378:metabolism / Q000502:physiology*; D014162:Transfection
D048868:Adaptor Proteins, Signal Transducing; D000906:Antibodies; D002135:Calcium-Binding Proteins; C082729:Eps15 protein, mouse; C094318:Eps15-rs protein, mouse; D047908:Intracellular Signaling Peptides and Proteins; D010750:Phosphoproteins; D066246:ErbB Receptors