Oligodendrocyte-specific protein (OSP) is a major component of CNS myelin.
An oligodendrocyte-specific protein (OSP) cDNA was recently identified and found to be expressed primarily in oligodendrocytes and has a deduced amino acid sequence similar to that of peripheral myelin protein 22 (PMP-22). We raised antibodies against a synthetic peptide corresponding to OSP amino acid residues 179-194 which reacted with a 22 kd protein in mouse CNS. OSP immunoreactivity localized to spinal cord white matter tracts using immunohistochemistry in a similar distribution to that of MBP. OSP localized to CNS myelin biochemically with more than a 30-fold enrichment measured in purified myelin. We further purified the proteolipid fraction of myelin and determined that OSP contributes approximately 7% of total myelin protein making it the third most abundant protein in CNS myelin. No binding was found to several agglutinins or a HNK1-specific antibody suggesting that OSP is not a glycoprotein.
Bronstein
J M
JM
Department of Neurology, University of California, Los Angeles School of Medicine, 90024, USA. jbronste@ucla.edu
Micevych
P E
PE
Chen
K
K
D000818:Animals; D001921:Brain / Q000166:cytology*; D007150:Immunohistochemistry; D051379:Mice; D009185:Myelin Proteins / Q000032:analysis* / Q000737:chemistry; D009186:Myelin Sheath / Q000648:ultrastructure*; D018992:Myelin-Associated Glycoprotein / Q000032:analysis* / Q000737:chemistry; D063308:Myelin-Oligodendrocyte Glycoprotein; D009836:Oligodendroglia / Q000166:cytology*; D013116:Spinal Cord / Q000166:cytology*
C571327:Mog protein, mouse; D009185:Myelin Proteins; D018992:Myelin-Associated Glycoprotein; D063308:Myelin-Oligodendrocyte Glycoprotein; C489545:Pmp22 protein, mouse