title: Direct injection HPLC method for the determination of phenylbutazone and oxyphenylbutazone in serum using a semipermeable surface column.
abstract: A direct injection HPLC method has been developed for the determination of phenylbutazone and its active metabolite oxyphenylbutazone in serum using a semipermeable surface (SPS) column. The method is easy to perform and requires 20 microliters of a filtered serum sample. The chromatographic time is less than 13 min using a mobile phase of 15:85 v/v acetonitrile-0.05M phosphate buffer pH 7.5. The method was linear in the range 0.5-20 micrograms ml-1 (r > 0.99, n = 6) with R.S.D. less than 6%. Interday and intraday variability were found to be less than 8.3%. The limit of quantitation and detection were 0.5 and 0.25 microgram ml-1 (s/n > 3), respectively, for both drug and metabolite.
authors.affiliation: Department of Medicinal Chemistry, College of Pharmacy, University of Georgia, Athens 30602-2352, USA.
authors.forename: A
authors.initials: A
authors.lastname: Haque
authors.forename: J T
authors.initials: JT
authors.lastname: Stewart
mesh_terms: D000818:Animals; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000097:blood / Q000737:chemistry / Q000302:isolation & purification*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D006736:Horses; D010113:Oxyphenbutazone / Q000097:blood / Q000737:chemistry / Q000302:isolation & purification*; D010653:Phenylbutazone / Q000097:blood / Q000737:chemistry / Q000302:isolation & purification*
chemical_list: D000894:Anti-Inflammatory Agents, Non-Steroidal; D010653:Phenylbutazone; D010113:Oxyphenbutazone