title: Contribution to the physiological characterization of glycerol active uptake in Saccharomyces cerevisiae.
abstract: Evidence is presented here that in Saccharomyces cerevisiae IGC 3507, grown either on glycerol, ethanol or acetate, glycerol is transported by a high affinity uptake system of the electrogenic proton symport type, with Km of 1.7 +/- 0.7 mM, Vmax 441 +/- 19 micromolh(-1) g(-1) dry weight and a stoichiometry of 1:1 proton per molecule of glycerol, at 30 degrees C and pH 5.0. No competitors were found among other polyols and sugars. Glycerol maximum accumulation ratios followed p.m.f. with extracellular pH. CCCP prevented glycerol accumulation, and inhibited uptake. NaCl did not interfere with H+/glycerol kinetics and energetics. This transport system was shown to be under glucose repression and inactivation. Glucose-grown cells presented, instead, a lower affinity permease for glycerol, probably a facilitated diffusion. Growth on glucose in the presence of NaCl did not induce the high affinity carrier. The stringent control of cell physiological condition over induction suggests for glycerol proton symport rather a physiological role connected with growth under gluconeogenic conditions.
authors.affiliation: Department of Biology, University of Minho, Braga, Portugal.
authors.forename: F
authors.initials: F
authors.lastname: Lages
authors.forename: C
authors.initials: C
authors.lastname: Lucas
mesh_terms: D001693:Biological Transport, Active / Q000187:drug effects; D002258:Carbonyl Cyanide m-Chlorophenyl Hydrazone / Q000494:pharmacology; D002851:Chromatography, High Pressure Liquid; D000431:Ethanol / Q000378:metabolism; D005947:Glucose / Q000378:metabolism / Q000494:pharmacology; D005990:Glycerol / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D017136:Ion Transport / Q000187:drug effects; D007700:Kinetics; D011522:Protons; D012441:Saccharomyces cerevisiae / Q000378:metabolism*; D012965:Sodium Chloride / Q000494:pharmacology
chemical_list: D011522:Protons; D000431:Ethanol; D012965:Sodium Chloride; D002258:Carbonyl Cyanide m-Chlorophenyl Hydrazone; D005947:Glucose; D005990:Glycerol