Effects of proglumide on cholecystokinin-8-induced exocrine and endocrine pancreatic responses in conscious sheep.
The effects of cholecystokinin (CCK)-8, either alone or together with the CCK antagonist, proglumide on both exocrine and endocrine pancreatic responses were examined in conscious sheep. Intravenous infusions of CCK-8 (120 pmol/kg/min for 40 min) with vehicle (0 mumol/kg/min proglumide) significantly increased both amylase output in pancreatic juice and plasma insulin concentrations (P < 0.05). Concomitant infusions of proglumide (5-40 mumol/kg/min for 50 min) inhibited both amylase and insulin secretory responses induced by CCK-8 infusion. The antagonistic effects of proglumide occurred in a dose-dependent manner, and proglumide infusion at dose of 20 mumol/kg/min or above simultaneously inhibited CCK-8-induced amylase and insulin responses. In conclusion, although the type of receptor involved is not characterized at present, exogenously infused CCK-8 acts on B cells via a CCK-receptor-mediated mechanism and induces insulin secretion in sheep.
Mineo
H
H
Faculty of Veterinary Medicine, Rakuno Gakuen University, Hokkaido, Japan.
Iwaki
N
N
Kogishi
K
K
Onaga
T
T
Kato
S
S
Zabielski
R
R
D000681:Amylases / Q000378:metabolism; D000818:Animals; D001786:Blood Glucose / Q000378:metabolism; D004305:Dose-Response Relationship, Drug; D005934:Glucagon / Q000097:blood / Q000378:metabolism; D007328:Insulin / Q000097:blood / Q000378:metabolism; D000078790:Insulin Secretion; D007515:Islets of Langerhans / Q000187:drug effects / Q000378:metabolism; D008297:Male; D010179:Pancreas / Q000187:drug effects* / Q000378:metabolism*; D010189:Pancreatic Juice / Q000378:metabolism; D011377:Proglumide / Q000008:administration & dosage / Q000494:pharmacology*; D011949:Receptors, Cholecystokinin / Q000187:drug effects / Q000502:physiology; D012756:Sheep; D012844:Sincalide / Q000037:antagonists & inhibitors* / Q000494:pharmacology*
D001786:Blood Glucose; D007328:Insulin; D011949:Receptors, Cholecystokinin; D005934:Glucagon; D000681:Amylases; D011377:Proglumide; D012844:Sincalide