title: Concentration of tissue inhibitor of metalloproteinases (TIMP)-1 in ovine follicular fluid and serum.
abstract: Tissue inhibitor of metalloproteinases (TIMP)-1 mRNA and protein localize within granulosal cells of post-gonadotropin-surge follicles and luteal tissue in ewes. Our objectives were to test the hypotheses that 1) follicular fluid concentration of TIMP-1 increases following a gonadotropin surge induced by LHRH agonist (Exp. 1) and 2) luteal status affects peripheral serum concentration of TIMP-1 (Exp. 2 and 3). In Exp. 1, the concentration of TIMP-1 within antral fluid from post-surge follicles (28.7 +/- 6.65 microg/mL) was greater (P < .02) than from pre-surge follicles (2.37 +/- 2.47 microg/mL). In Exp. 2, serum concentration of TIMP-1 did not differ among d 0 to 6 (1.27 +/- .55 microg/mL) of the estrous cycle or among periods of luteal maintenance (1.29 +/- .06 microg/mL), spontaneous luteal regression (1.19 +/- .09 microg/mL), or luteal development (1.22 +/- .08 microg/mL). However, serum concentration of TIMP-1 was greater ( P < .001) during the period of luteal maintenance (1.14 +/- .04 microg/mL) than during PGF2alpha-induced luteolysis (d 26; .85 +/- .06 microg/mL) and induced luteal absence (d 27 to 33; .95 +/- .05 microg/mL). In Exp. 3, ewes (n = 14) were bled daily from d 1 to 19 (d 0 = estrus) and at 12-min intervals for 6 h on d 3, 10, and 17. Although concentration of TIMP-1 varied considerably within and among ewes, mean concentration of TIMP-1 per ewe per day increased ( P < .05) from d 3 to 17. These data indicate that follicular fluid concentration of TIMP-1 increases following a gonadotropin surge, but the contribution of ovarian derived TIMP-1 to peripheral serum concentration is negligible.
authors.affiliation: Department of Animal Science, University of Missouri-Columbia, 65211, USA.
authors.forename: E W
authors.initials: EW
authors.lastname: McIntush
authors.forename: D H
authors.initials: DH
authors.lastname: Keisler
authors.forename: M F
authors.initials: MF
authors.lastname: Smith
mesh_terms: D000818:Animals; D015237:Dinoprost / Q000494:pharmacology; D004971:Estrus / Q000502:physiology; D005260:Female; D015571:Follicular Fluid / Q000737:chemistry* / Q000378:metabolism; D007987:Gonadotropin-Releasing Hormone / Q000819:agonists; D008183:Luteal Phase / Q000502:physiology; D011374:Progesterone / Q000097:blood; D012333:RNA, Messenger / Q000032:analysis / Q000235:genetics; D011863:Radioimmunoassay / Q000662:veterinary; D012044:Regression Analysis; D012756:Sheep / Q000097:blood / Q000378:metabolism*; D019715:Tissue Inhibitor of Metalloproteinase-1 / Q000032:analysis* / Q000097:blood* / Q000235:genetics
chemical_list: D012333:RNA, Messenger; D019715:Tissue Inhibitor of Metalloproteinase-1; D007987:Gonadotropin-Releasing Hormone; D011374:Progesterone; D015237:Dinoprost