Exchange of viral promoter/enhancer elements with heterologous regulatory sequences generates targeted hybrid long terminal repeat vectors for gene therapy of melanoma.
To generate transcriptionally targeted vectors, tissue-specific elements of the human tyrosinase promoter were exchanged with corresponding viral elements in the Moloney murine leukemia virus long terminal repeat (LTR). From these experiments, a vesicular stomatitis virus type G pseudotyped, hybrid LTR vector that contained three tyrosinase enhancer elements and gave high-level, tightly tissue-specific expression at high titers (3 x 10(7) CFU/ml) was constructed.
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Richard Dimbleby Department of Cancer Research/ICRF Laboratory, Rayne Institute, St. Thomas' Hospital, London, United Kingdom.
Eisen
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Hart
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Vile
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D000818:Animals; D004742:Enhancer Elements, Genetic; D018390:Gene Targeting; D005809:Genes, Regulator; D005814:Genes, Viral; D015316:Genetic Therapy; D005822:Genetic Vectors; D006801:Humans; D009052:Leukemia Virus, Murine / Q000235:genetics*; D008545:Melanoma / Q000628:therapy*; D051379:Mice; D014442:Monophenol Monooxygenase / Q000235:genetics; D011401:Promoter Regions, Genetic; D012091:Repetitive Sequences, Nucleic Acid; D014407:Tumor Cells, Cultured; D014721:Vesicular stomatitis Indiana virus / Q000235:genetics
D014442:Monophenol Monooxygenase