Expression of cereolysine AB genes in Bacillus anthracis vaccine strain ensures protection against experimental hemolytic anthrax infection.
The cereolysin AB genes from Bacillus cereus VKM-B164 have been expressed in Bacillus anthracis strains: virulent H-7 (PXO1, PXO2), vaccine STI-1 (PXO1), 221 (without its own plasmids). Expression was achieved by cloning the genes in a high copy number plasmid pE194. This construct was integrated with host genomes in amplified form. Gold hamsters were vaccinated with parental and recombinant B. anthracis STI-1 and 221 strains and challenged with virulent ones subcutaneously. Gold hamsters vaccinated with 221 strains showed, absence of protection. STI-1 immunisation protected against the H-7 strain, but did not protect against the recombinant strain. STI-1 recombinant strain protected gold hamsters against the H-7 as well as the recombinant H-7 strains. The results describe the modulation of immunopathogenic properties of B. anthracis due to expression of cereolysin AB genes.
Pomerantsev
A P
AP
State Research Centre for Applied Microbiology, Obolensk, Moscow Region, Russia.
Staritsin
N A
NA
Mockov YuV
Marinin
L I
LI
D000818:Animals; D000881:Anthrax / Q000517:prevention & control*; D001408:Bacillus anthracis / Q000235:genetics / Q000276:immunology* / Q000378:metabolism*; D001409:Bacillus cereus / Q000235:genetics*; D001426:Bacterial Proteins / Q000096:biosynthesis* / Q000235:genetics*; D001428:Bacterial Vaccines / Q000235:genetics* / Q000276:immunology / Q000627:therapeutic use*; D006224:Cricetinae; D004269:DNA, Bacterial / Q000032:analysis / Q000235:genetics; D018274:Electroporation; D005798:Genes, Bacterial; D006460:Hemolysin Proteins / Q000096:biosynthesis; D008647:Mesocricetus; D051379:Mice; D009693:Nucleic Acid Hybridization; D010957:Plasmids / Q000235:genetics; D014169:Transformation, Bacterial
D001426:Bacterial Proteins; D001428:Bacterial Vaccines; D004269:DNA, Bacterial; D006460:Hemolysin Proteins; C006953:cereolysin