Isolation of a putative prolyl-tRNA synthetase (CaPRS) gene from Candida albicans.
We have isolated a 4.0-kb fragment from a genomic library of Candida albicans which contained two open reading frames (ORFs). One of them is homologous to a prolyl-tRNA synthetase that catalyses the charging of a specific tRNA by proline (CaPRS). A deduced sequence of 575 amino acids representing a polypeptide of 66.2 kDa was determined. A FASTA search indicated that the CaPRSp had an overall similarity of 54.4% with the product of a Saccharomyces cerevisiae ORF (YER087) and 43.8% with the prolyl-tRNA synthetase of Escherichia coli (COLIPRO). Consensus Class II aminoacyl-tRNA synthetase sequences were identified by the PROSITE program. CaPRS was localized to chromosome R of the C. albicans genome and CaPRS DNA hybridized to a major RNA transcript of 1.7 kb under all conditions tested.
Sentandreu
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M
Departament de Microbiología i Ecología, Facultat de Farmacia, València, Spain.
Elorza
M V
MV
Sentandreu
R
R
D000595:Amino Acid Sequence; D000604:Amino Acyl-tRNA Synthetases / Q000737:chemistry / Q000235:genetics* / Q000302:isolation & purification*; D001483:Base Sequence; D002176:Candida albicans / Q000201:enzymology* / Q000235:genetics*; D015825:Chromosomes, Fungal / Q000737:chemistry; D003001:Cloning, Molecular; D004271:DNA, Fungal / Q000378:metabolism; D015966:Gene Expression Regulation, Fungal; D005800:Genes, Fungal; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid
D004271:DNA, Fungal; D000604:Amino Acyl-tRNA Synthetases; C100134:prolyl T RNA synthetase