title: The antigenic sites of trichosanthin, a ribosome-inactivating protein with multiple pharmacological properties.
abstract: Trichosanthin (TCS) fragments were produced by Tn1000 deletion mutagenesis and by cyanogen bromide (CNBr) cleavage and their immunoreactivity was examined by incubating with various antibodies. Twelve C-terminally truncated TCS variants were successfully synthesized under the control of a T7 RNA driven promoter. The smallest antigenic fragment mapped corresponded to the N-terminal 20 amino acids (aa). Six CNBr fragments of TCS were created and identified by electrospray mass spectrometry and N-terminal sequencing. Three antigenic fragments corresponding to aa 1-72, 101-152 and 153-246, respectively were mapped. Fragments corresponding to aa 1-72 and 153-246 were immunoreactive to the same monoclonal antibody showing they are components of a discontinuous epitope. On the other hand, the fragment containing aa 73-100 was not detected by any of the antibodies used.
authors.affiliation: Department of Biochemistry, The Chinese University of Hong Kong, Shatin, NT.
authors.forename: S
authors.initials: S
authors.lastname: Mulot
authors.forename: K K
authors.initials: KK
authors.lastname: Chung
authors.forename: X B
authors.initials: XB
authors.lastname: Li
authors.forename: C C
authors.initials: CC
authors.lastname: Wong
authors.forename: T B
authors.initials: TB
authors.lastname: Ng
authors.forename: P C
authors.initials: PC
authors.lastname: Shaw
mesh_terms: D000818:Animals; D004251:DNA Transposable Elements; D018604:Epitope Mapping; D009154:Mutation; D011817:Rabbits; D015978:Trichosanthin / Q000276:immunology*
chemical_list: D004251:DNA Transposable Elements; D015978:Trichosanthin