[Chromosomal aberrations induced in human spermatozoa by mitomycin C].
Human spermatozoa were exposed to Mitomycin C (MM() at the concentrations of 7.5, 15, 3O micrograms/ml for 1 hour respectively before interspecific in vitro fertilization with zona-free hamster ova. The first cleavage metaphases was analyzed to investigate MMC-induced effects on human spermatozoa. The results showed a distinct dose-response relationship in the induction of chromosomal aberrations. The chromatid aberrations were the predominant types in MMC-induced changes. Some chromosome-type aberrations were also observed. This suggested that the induced effects of MMC on human spermatozoa were some different from that of ultraviolet light-like clastogens on somatic cells. 36.89% of MMC-induced aberrations were rejoining-type. This indicated that the DNA repair systems present in golden hamster oocytes were effective in repairing MMC-induced DNA lesions in human spermatozoa.
Huang
T
T
Shantou University Medical College.
Liu
X
X
Cai
M
M
Huang
J
J
D000818:Animals; D000903:Antibiotics, Antineoplastic / Q000633:toxicity*; D002869:Chromosome Aberrations; D006224:Cricetinae; D004305:Dose-Response Relationship, Drug; D005260:Female; D006801:Humans; D008297:Male; D016685:Mitomycin / Q000633:toxicity*; D013094:Spermatozoa / Q000187:drug effects* / Q000648:ultrastructure
D000903:Antibiotics, Antineoplastic; D016685:Mitomycin