Identification of a novel gene, PSD, adjacent to NFKB2/lyt-10, which contains Sec7 and pleckstrin-homology domains.
We have identified a novel human gene on chromosome 10q24 located contiguously to the 3' end of the NFKB2/lyt-10 gene in a tail to tail arrangement. We describe here a cDNA of 4307 bp, isolated from an adult human brain cDNA library, which contains an open reading frame encoding a putative protein of 645 amino acids with a predicted molecular weight of 71 kDa. Database homology searches indicate that this is a novel gene coding for a putative protein containing two discrete domains with significant homology to the Sec7 and pleckstrin-homology (PH) domains, respectively. We named this gene PSD (plekstrin-Sec7 domains gene). Northern blot analysis of a panel of RNAs from normal human tissues using the PSD cDNA as probe revealed the presence of three different tissue-specific transcripts of approximately 4.3, 2.3, and 1.8 kb, the longest of which is expressed only in brain. Our data suggest that the PSD gene may code for a protein related to a recently identified protein family containing both the Sec7 and the PH domains thought to be involved in signaling transduction processes.
Perletti
L
L
Laboratorio di Ematologia Sperimentale e Genetica Molecolare, Università di Milano, Ospedale Maggiore IRCCS, Milan, Italy.
Talarico
D
D
Trecca
D
D
Ronchetti
D
D
Fracchiolla
N S
NS
Maiolo
A T
AT
Neri
A
A
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D001798:Blood Proteins / Q000235:genetics; D015152:Blotting, Northern; D001921:Brain / Q000502:physiology; D002879:Chromosomes, Human, Pair 10; D003001:Cloning, Molecular; D005656:Fungal Proteins / Q000235:genetics; D020662:Guanine Nucleotide Exchange Factors; D006801:Humans; D008969:Molecular Sequence Data; D016328:NF-kappa B / Q000235:genetics; D052003:NF-kappa B p52 Subunit; D009419:Nerve Tissue Proteins / Q000235:genetics* / Q000378:metabolism*; D010750:Phosphoproteins; D014176:Protein Biosynthesis; D011506:Proteins / Q000235:genetics* / Q000378:metabolism*; D012326:RNA Splicing; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D014018:Tissue Distribution; D014158:Transcription, Genetic
D001798:Blood Proteins; D005656:Fungal Proteins; D020662:Guanine Nucleotide Exchange Factors; D016328:NF-kappa B; D052003:NF-kappa B p52 Subunit; D009419:Nerve Tissue Proteins; C109970:PSD protein, human; D010750:Phosphoproteins; D011506:Proteins; C066755:Sec7 guanine nucleotide exchange factors; C039077:platelet protein P47