title: Inhibitory properties of double-helix-forming circular oligonucleotides.
abstract: Several circular oligonucleotides were synthesized and characterized by electrospray ionization mass spectrometry. Experiments on termination of primer extension catalysed by DNA polymerases, Klenow fragment and Tth have demonstrated that a double helix forming circular 2'-deoxyribooligomer containing a 25mer sequence complementary to the target single-stranded DNA along with a 34mer random mismatching stretch appears to be a potent inhibitor of replication in vitro. Studies on inhibition of luciferase gene expression in a cell-free transcription-translation system have shown that a duplex forming circular 2'-deoxyribooligonucleotide containing a 25mer sequence complementary to the target mRNA and a 14mer random mismatching stretch can serve as an effective antisense compound as a standard linear complementary oligomer. Features of double helix forming circular oligonucleotides composed of 2'-deoxyribonucleosides seem to be useful for the design of new antigene and antisense agents.
authors.affiliation: Department of Pharmaceutical Chemistry, University of Kuopio, FIN-70211 Kuopio, Finland.
authors.forename: E
authors.initials: E
authors.lastname: Azhayeva
authors.forename: A
authors.initials: A
authors.lastname: Azhayev
authors.forename: S
authors.initials: S
authors.lastname: Auriola
authors.forename: U
authors.initials: U
authors.lastname: Tengvall
authors.forename: A
authors.initials: A
authors.lastname: Urtti
authors.lastname: Lönnberg
mesh_terms: D001483:Base Sequence; D002851:Chromatography, High Pressure Liquid; D004256:DNA Polymerase I / Q000378:metabolism; D004261:DNA Replication / Q000187:drug effects*; D004277:DNA, Single-Stranded / Q000235:genetics; D018390:Gene Targeting / Q000379:methods*; D017930:Genes, Reporter; D008156:Luciferases / Q000096:biosynthesis / Q000235:genetics; D013058:Mass Spectrometry; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation; D009841:Oligonucleotides / Q000138:chemical synthesis / Q000737:chemistry / Q000494:pharmacology*; D016376:Oligonucleotides, Antisense / Q000138:chemical synthesis / Q000737:chemistry / Q000494:pharmacology*; D012333:RNA, Messenger / Q000037:antagonists & inhibitors*; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis / Q000235:genetics; D013698:Templates, Genetic
chemical_list: D004277:DNA, Single-Stranded; D009841:Oligonucleotides; D016376:Oligonucleotides, Antisense; D012333:RNA, Messenger; D011993:Recombinant Fusion Proteins; D008156:Luciferases; D004256:DNA Polymerase I