Matrix-assisted laser desorption ionization mass spectrometry of membrane proteins: demonstration of a simple method to determine subunit molecular weights of hydrophobic subunits.
Matrix-assisted laser desorption ionization (MALDI) mass spectrometry has been used to obtain accurate molecular weight information for each subunit of several hydrophobic integral membrane proteins: cytochrome bo3 (4 subunits) and cytochrome bd (2 subunits) from E. coli, and the bc1 complex (3 subunits) and the cytochrome c oxidase (3 subunits) from Rhodobacter sphaeroides. The results demonstrate that the MALDI method is a convenient, quick, sensitive and reliable means for obtaining the molecular masses of the subunits of purified multisubunit membrane proteins.
Ghaim
J B
JB
Department of Biochemistry, University of Illinois, Urbana 61801, USA.
Tsatsos
P H
PH
Katsonouri
A
A
Mitchell
D M
DM
Salcedo-Hernandez
R
R
Gennis
R B
RB
D003573:Cytochrome b Group; D003580:Cytochromes / Q000737:chemistry*; D045222:Electron Transport Chain Complex Proteins; D014450:Electron Transport Complex III / Q000737:chemistry; D003576:Electron Transport Complex IV / Q000737:chemistry; D004591:Electrophoresis, Polyacrylamide Gel; D004926:Escherichia coli / Q000737:chemistry / Q000201:enzymology; D029968:Escherichia coli Proteins; D008565:Membrane Proteins / Q000737:chemistry* / Q000302:isolation & purification; D008970:Molecular Weight; D010088:Oxidoreductases / Q000737:chemistry*; D011487:Protein Conformation; D012242:Rhodobacter sphaeroides / Q000737:chemistry / Q000201:enzymology; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
D003573:Cytochrome b Group; D003580:Cytochromes; D045222:Electron Transport Chain Complex Proteins; D029968:Escherichia coli Proteins; D008565:Membrane Proteins; C083850:cytochrome bo3, E coli; D010088:Oxidoreductases; C040077:cytochrome bd terminal oxidase complex, E coli; D003576:Electron Transport Complex IV; D014450:Electron Transport Complex III