A functional study of the salmon GnRH promoter.
The Pa and Pb promoters of the Atlantic salmon (Salmo salar) GnRH gene were fused together or individually to the Escherichia coli lacZ gene, and their transcriptional activities were measured in transient expression assays in zebrafish (Danio rerio). In 48-hour embryos, both promoters were preferentially expressed in the brain, whereas a cytomegalovirus (CMV) promoter-lacZ fusion gene displayed high levels of activity in nonbrain tissues. Pa and Pb exhibited different cell specificity in the forebrain. Pb was active in large neuron-like cells exclusive in the olfactory placode region, whereas Pa appeared active in nonneuron-like cells in the forebrain. In Atlantic salmon forebrain tissue, both Pa and Pb exhibited endogenous activity, as assessed by reverse transcriptase-polymerase chain reaction (RT-PCR) analysis. However, only the Pb transcript contained the prepro-GnRH exon II-IV sequences, suggesting that Pa activity may not be related to GnRH production in this species.
Husebye
H
H
Department of Biochemistry, Physiology and Nutrition, Norwegian College of Veterinary Medicine, Oslo, Norway.
Collas
P
P
Aleström
P
P
D000818:Animals; D030801:Animals, Genetically Modified; D004625:Embryo, Nonmammalian / Q000378:metabolism; D004926:Escherichia coli / Q000201:enzymology; D005091:Exons; D007987:Gonadotropin-Releasing Hormone / Q000096:biosynthesis* / Q000235:genetics*; D009474:Neurons / Q000378:metabolism; D009833:Olfactory Pathways / Q000378:metabolism; D016133:Polymerase Chain Reaction; D011401:Promoter Regions, Genetic; D016548:Prosencephalon / Q000378:metabolism; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis; D012474:Salmon / Q000235:genetics*; D001616:beta-Galactosidase / Q000096:biosynthesis
D011993:Recombinant Fusion Proteins; D007987:Gonadotropin-Releasing Hormone; D001616:beta-Galactosidase