Developmental changes in UDP-N-acetylglucosamine 2-epimerase activity of rat and guinea-pig liver.
The activity of UDP-N-acetylglucosamine 2-epimerase was determined in the liver of rats and guinea-pigs of different ages. The activity of this enzyme in rats was low at birth, increased to a maximum value on day 15, and fell gradually until day 30. Thereafter, it increased up to the 60th day. The activity profile of the enzyme from guinea-pig liver was very similar. However, guinea-pig activity was 2-5 times lower than in rats. Both rats and guinea-pigs displayed similar liver sialic acid contents which increased from birth to 2 months of age. Rats also showed a N-glycolylneuraminic acid content that decreased from birth to 2 months. From these results we can inferred that postnatal UDP-N-acetylglucosamine 2-epimerase activity seems to be correlated with age and the developmental states of rats and guinea-pigs.
Gal
B
B
Departamento de Bioquímica y Biología Molecular, Facultad de Biología, Universidad de Salamanca, Spain.
Ruano
M J
MJ
Puente
R
R
García-Pardo
L A
LA
Rueda
R
R
Gil
A
A
Hueso
P
P
D000818:Animals; D000831:Animals, Newborn; D002238:Carbohydrate Epimerases / Q000378:metabolism; D029968:Escherichia coli Proteins; D005260:Female; D006168:Guinea Pigs; D008099:Liver / Q000201:enzymology* / Q000254:growth & development* / Q000378:metabolism; D008297:Male; D008892:Milk / Q000378:metabolism; D019158:N-Acetylneuraminic Acid / Q000378:metabolism; D009438:Neuraminic Acids / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D013045:Species Specificity
D029968:Escherichia coli Proteins; D009438:Neuraminic Acids; C032592:N-glycolylneuraminic acid; D002238:Carbohydrate Epimerases; C100033:UDP acetylglucosamine-2-epimerase; C083982:wecB protein, E coli; D019158:N-Acetylneuraminic Acid