Amplification of bacitracin transporter genes in the bacitracin producing Bacillus licheniformis.
We have amplified the previously cloned and sequenced genes of the bacitracin exporter (bcr), a member of the ATP-binding transport protein family, within the chromosome of the bacitracin producing Bacillus licheniformis. Amplification of the transporter genes was followed by greatly increased bacitracin resistance. Antibiotic production was enhanced at a low level of bcr genes amplification. An enlarged increase in the copy number of the bcr genes negatively affects the overall growth of bacteria.
Podlesek
Z
Z
Biotechnical Faculty, Department of Biology, University of Ljubljana, Slovenia.
Herzog
B
B
Comino
A
A
D018528:ATP-Binding Cassette Transporters / Q000235:genetics* / Q000378:metabolism; D001407:Bacillus / Q000187:drug effects / Q000235:genetics*; D001414:Bacitracin / Q000096:biosynthesis / Q000378:metabolism*; D001692:Biological Transport; D003001:Cloning, Molecular; D004352:Drug Resistance, Microbial; D015964:Gene Expression Regulation, Bacterial; D005798:Genes, Bacterial; D010957:Plasmids
D018528:ATP-Binding Cassette Transporters; D001414:Bacitracin