title: Fertilization and pregnancy after assisted oocyte activation and intracytoplasmic sperm injection in a case of round-headed sperm associated with deficient oocyte activation capacity.
abstract: OBJECTIVE
  To investigate a method of assisted activation of human oocytes for the treatment of infertility resulting from globozoospermia associated with deficient oocyte activation capacity.
  
  
  METHODS
  The mouse oocyte activation test was used to analyze the oocyte activation capacity of the sperm cells of a patient with globozoospermia. Fresh donor human oocytes were used for determining the most appropriate procedure for oocyte activation.
  
  
  METHODS
  Infertility Center, University Hospital of Ghent.
  
  
  METHODS
  A couple with infertility resulting from globozoospermia.
  
  
  METHODS
  Intracytoplasmic sperm injection, assisted oocyte activation, and embryo transfer.
  
  
  METHODS
  Oocyte activation and fertilization rates, implantation, and pregnancy.
  
  
  RESULTS
  Deficiency in oocyte activation capacity was found in the sperm of a patient with globozoospermia. This deficiency was proven by the mouse oocyte activation test and was confirmed further by lack of activation of human oocytes after simple sperm injection. Only human oocytes that were injected with sperm and subjected to calcium chloride and ionophore treatment underwent activation. Transfer of embryos obtained by this procedure of assisted oocyte activation resulted in an ongoing pregnancy.
  
  
  CONCLUSIONS
  Assisted oocyte activation of human oocytes is useful when globozoospermia is associated with absence of oocyte activation capacity in the sperm. These cases can be identified by the mouse oocyte activation test.
authors.affiliation: Department of Gynaecology and Obstetrics, University Hospital, Ghent, Belgium. ANDREI.RYBOUCHKIN@RUG.AC.BE
authors.forename: A V
authors.initials: AV
authors.lastname: Rybouchkin
authors.forename: F
authors.initials: F
authors.lastname: Van der Straeten
authors.forename: J
authors.initials: J
authors.lastname: Quatacker
authors.forename: P
authors.initials: P
authors.lastname: De Sutter
authors.forename: M
authors.initials: M
authors.lastname: Dhont
mesh_terms: D000328:Adult; D000818:Animals; D000001:Calcimycin / Q000494:pharmacology*; D003593:Cytoplasm; D005260:Female; D005307:Fertilization in Vitro / Q000379:methods*; D006801:Humans; D007248:Infertility, Male / Q000473:pathology* / Q000628:therapy*; D007476:Ionophores / Q000494:pharmacology*; D008297:Male; D051379:Mice; D008845:Microinjections; D018587:Oocyte Donation; D009865:Oocytes / Q000187:drug effects* / Q000502:physiology*; D011247:Pregnancy; D013094:Spermatozoa / Q000002:abnormalities*
chemical_list: D007476:Ionophores; D000001:Calcimycin