title: Immunochemical methods for identification of Babesia bovis antigens expressed on the erythrocyte surface.
abstract: Intraerythrocytic parasites, such as Babesia bovis, modify the erythrocyte plasma membrane structurally, antigenically, and functionally. For such parasites the infected erythrocyte surface also is thought to be a primary site for interaction with the host immune system. These properties demand characterization of the various alterations to understand the overall host-parasite interaction, immunity to disease or infection, and bases for parasite persistence. A paucity of adequate methods exists for characterization of parasite-derived components of the parasitized erythrocyte surface. To facilitate such studies we developed or modified several techniques to detect, identify, and localize parasite-induced alterations on the B. bovis-infected erythrocyte surface. These methods, which we present here, should be adaptable to a variety of intraerythrocytic parasite-host combinations.
authors.affiliation: Department of Pathobiology, College of Veterinary Medicine, University of Florida, Gainesville, Florida, 32611-0880, USA. allredD@mail.vetmed.ufl.edu
authors.forename: D R
authors.initials: DR
authors.lastname: Allred
mesh_terms: D000818:Animals; D000953:Antigens, Protozoan / Q000032:analysis* / Q000096:biosynthesis; D016793:Babesia bovis / Q000502:physiology*; D002417:Cattle; D004591:Electrophoresis, Polyacrylamide Gel; D004910:Erythrocyte Membrane / Q000469:parasitology* / Q000648:ultrastructure; D005455:Fluorescent Antibody Technique; D007150:Immunohistochemistry / Q000379:methods; D007202:Indicators and Reagents; D011817:Rabbits
chemical_list: D000953:Antigens, Protozoan; D007202:Indicators and Reagents