title: Genotypic analysis of the 5'-untranslated region of a pestivirus strain isolated from human leucocytes.
abstract: The 5'-untranslated genomic region of the pestivirus strain Europa, originated in human leucocytes and previously identified as bovine diarrhea virus (BVDV), was amplified by reverse transcription-PCR and sequenced. Analyses based on primary nucleotide sequence homology and on secondary palindromic sequence structures characteristic to genotypes revealed that this human isolate should be assigned to a novel genotype of pestivirus, type Ic. This newly emerged genotype was related to, but distinguishable from the three known BVDV genotypes, Ia, Ib and II. Three other bovine field isolates of BVDV originated from Germany were also found to belong to this new genotype Ic. Within pestivirus genotype Ic strains, the overall nucleotide sequence homology was 95-96%, and 88-92%, 88-90% and 77-79% with the other BVDV genotypes Ia, Ib and II, respectively. With the strains from border disease virus (genotype III) and hog cholera virus (genotype IV), homologies were less than 75%.
authors.affiliation: Special Pathology and Veterinary Medical Clinic Institute, Faculty of Veterinary Medicine, The University of Milan, Italy.
authors.forename: M
authors.initials: M
authors.lastname: Giangaspero
authors.forename: R
authors.initials: R
authors.lastname: Harasawa
authors.forename: A
authors.initials: A
authors.lastname: Verhulst
mesh_terms: D000818:Animals; D002417:Cattle; D017931:DNA Primers; D001908:Diarrhea Viruses, Bovine Viral / Q000235:genetics*; D005838:Genotype; D006801:Humans; D007962:Leukocytes / Q000821:virology*; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation; D010576:Pestivirus / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D012367:RNA, Viral / Q000737:chemistry / Q000235:genetics*; D017423:Sequence Analysis, RNA; D012689:Sequence Homology, Nucleic Acid
chemical_list: D017931:DNA Primers; D012367:RNA, Viral