Nuclear location and cell cycle regulation of the BRCA2 protein.
Women carrying a germ-line mutation in the BRCA1 or BRCA2 genes have a high risk of developing breast cancer, and loss of the wild-type allele in tumors suggests that these genes function as tumor suppressor genes. The BRCA2 gene encodes a 3418-amino acid protein with no significant sequence similarity to any known protein. To begin to elucidate the cellular role of BRCA2, we have raised antibodies to the BRCA2 protein and used these to study its subcellular localization and expression. We show that BRCA2 is a nuclear protein expressed in response to cell proliferation and that BRCA2 expression is initiated before DNA synthesis.
Bertwistle
D
D
Cancer Research Campaign Centre for Cell and Molecular Biology, Chester Beatty Laboratories, The Institute of Cancer Research, London, United Kingdom.
Swift
S
S
Marston
N J
NJ
Jackson
L E
LE
Crossland
S
S
Crompton
M R
MR
Marshall
C J
CJ
Ashworth
A
A
D000818:Animals; D000906:Antibodies; D000911:Antibodies, Monoclonal; D000918:Antibody Specificity; D024682:BRCA2 Protein; D015153:Blotting, Western; D001943:Breast Neoplasms / Q000235:genetics; D002453:Cell Cycle / Q000502:physiology; D002467:Cell Nucleus / Q000737:chemistry / Q000502:physiology; D005260:Female; D006801:Humans; D009363:Neoplasm Proteins / Q000032:analysis* / Q000502:physiology*; D009687:Nuclear Proteins / Q000032:analysis* / Q000502:physiology*; D011817:Rabbits; D051381:Rats; D012307:Risk Factors; D013347:Subcellular Fractions / Q000737:chemistry; D014157:Transcription Factors / Q000032:analysis* / Q000502:physiology*
D000906:Antibodies; D000911:Antibodies, Monoclonal; D024682:BRCA2 Protein; D009363:Neoplasm Proteins; D009687:Nuclear Proteins; D014157:Transcription Factors