Expression pattern of the mitochondrial capsule selenoprotein mRNA in the mouse testis after puberty; in situ hybridization study.
Mitochondrial capsule selenoprotein (MCS) has been known as a structural protein of the mitochondrial sheath in spermatozoa. In this study, to determine the expression pattern of MCS mRNA in the mouse testis after puberty, in situ hybridization using digoxigenin-labeled RNA probes for MCS was performed in the testes of 8- and 20-week-old ICR mice. In the testes of both ages, MCS mRNA first appeared in step 3 round spermatids, gradually increased during early spermiogenesis, and persisted a high level until step 14 spermatids. After the step 14 spermatids, the signal began to decline and was weakly detected in steps 15-16 spermatids. On the other hand, compared with that in the testes of 8-week-old mice, MCS mRNA level in the testes of 20-week-old mice increased over 2-fold at stages VI-III, while it slightly increased at stages IV-V. These findings suggest that MCS gene transcription may be up-regulated after puberty in the mouse testis.
Nam
S Y
SY
Department of Veterinary Anatomy, Graduate School of Agriculture and Life Sciences, University of Tokyo, Japan.
Maeda
S
S
Ogawa
K
K
Kurohmaru
M
M
Hayashi
Y
Y
D000375:Aging / Q000378:metabolism; D000818:Animals; D018507:Gene Expression Regulation, Developmental; D008297:Male; D051379:Mice; D008813:Mice, Inbred ICR; D014176:Protein Biosynthesis; D011506:Proteins; D012333:RNA, Messenger / Q000096:biosynthesis; D051140:Selenoproteins; D012741:Sexual Maturation; D013087:Spermatids / Q000166:cytology / Q000502:physiology*; D013091:Spermatogenesis; D013737:Testis / Q000166:cytology / Q000254:growth & development / Q000502:physiology*; D014158:Transcription, Genetic
C484386:Mcsp protein, mouse; D011506:Proteins; D012333:RNA, Messenger; D051140:Selenoproteins