title: Enantiomeric determination of amino compounds with high sensitivity using the chiral reagents (+)- and (-)-1-(9-anthryl)-2-propyl chloroformate.
abstract: New chiral precolumn reagents, (+)- and (-)-1-(9-anthryl)-2-propyl chloroformate (APOC), are introduced for the chiral separation of amino acids and small peptides in capillary electrophoresis. Chiral separation of 17 amino acids and four small peptides as their diastereomeric 1-(9-anthryl)-2-propyl carbamate derivatives have been achieved by micellar electrokinetic chromatography. The detection limit for the derivatives is in the femtomole range with UV detection and in the attomole range with laser-induced fluorescence (LIF) detection. LIF detection was used to determine the enantiomeric excess of four APOC-derivatised peptides. The use of the new, anthracene-based reagents in conjunction with argon ion LIF makes enantiomeric determinations at ppm levels feasible. In this paper determinations below promille levels are performed without overloading the separation system.
authors.affiliation: Department of Analytical Chemistry, Arrhenius Laboratories for Natural Sciences, Stockholm University, Stockholm.
authors.forename: G
authors.initials: G
authors.lastname: Thorsén
authors.forename: A
authors.initials: A
authors.lastname: Engström
authors.forename: B
authors.initials: B
authors.lastname: Josefsson
mesh_terms: D000596:Amino Acids / Q000032:analysis; D000873:Anthracenes / Q000737:chemistry*; D019075:Electrophoresis, Capillary; D007202:Indicators and Reagents; D009842:Oligopeptides / Q000737:chemistry*; D012680:Sensitivity and Specificity; D013237:Stereoisomerism
chemical_list: C110229:1-(9-anthryl)-2-propyl chloroformate; D000596:Amino Acids; D000873:Anthracenes; D007202:Indicators and Reagents; D009842:Oligopeptides