title: Autoantibody formation against a 190-kDa antigen of the desmosomal plaque in pemphigus foliaceus.
abstract: We report changes in the antigen recognition pattern of sera from two pemphigus foliaceus patients with a long-term follow-up. The patients' sera were analysed by immunoblotting using different antigenic sources: cultured human keratinocytes, bovine tongue epithelium and a recombinant protein corresponding to the C-terminal end of the 230-kDa bullous pemphigoid antigen. While initial serum samples reacted exclusively with the 160-kDa desmoglein 1, the later sera reacted both with desmoglein 1 and a 190-kDa antigen immunolocalized to the desmosomal plaque, previously demonstrated to be recognized by sera of some patients with paraneoplastic pemphigus. IgG subclass analysis further showed that antidesmoglein 1 antibodies were of IgG1 and/or IgG4 subclasses, while anti-190-kDa antibodies were IgG3. The patients were free of malignancy.
authors.affiliation: Clinique Dermatologique, Hôpital Edouard Herriot, Lyon.
authors.forename: R
authors.initials: R
authors.lastname: Ghohestani
authors.forename: P
authors.initials: P
authors.lastname: Joly
authors.forename: D
authors.initials: D
authors.lastname: Gilbert
authors.forename: J F
authors.initials: JF
authors.lastname: Nicolas
authors.forename: E
authors.initials: E
authors.lastname: Thomine
authors.forename: E
authors.initials: E
authors.lastname: Cozzani
authors.forename: P H
authors.initials: PH
authors.lastname: Lauret
authors.forename: A
authors.initials: A
authors.lastname: Claudy
authors.forename: F
authors.initials: F
authors.lastname: Tron
mesh_terms: D000368:Aged; D001323:Autoantibodies / Q000096:biosynthesis*; D001324:Autoantigens / Q000276:immunology*; D003896:Desmosomes / Q000276:immunology*; D005260:Female; D019084:Fluorescent Antibody Technique, Indirect; D005500:Follow-Up Studies; D006801:Humans; D015151:Immunoblotting; D008297:Male; D016253:Microscopy, Immunoelectron; D008875:Middle Aged; D010392:Pemphigus / Q000276:immunology* / Q000473:pathology
chemical_list: D001323:Autoantibodies; D001324:Autoantigens