title: Effect of high-energy phosphates and free radical scavengers on replant survival in an ischemic extremity model.
abstract: In replantation surgery, preoperative and intraoperative ischemia can lead to irreversible changes that prevent reperfusion during the subsequent re-establishment of circulation. These changes are termed the no-reflow phenomenon. Ischemic phase damage was addressed by comparing the dose-response effects of controls vs. five different high-energy phosphate compounds on replanted limb survival. Reperfusion damage was evaluated via comparisons of controls with superoxide dismutase (SOD). Ischemic hindlimbs treated with high-energy phosphates displayed improved survival compared with controls. Limbs treated with SOD demonstrated no change in survival at 4 hours and improved survival at 8 hours. Combining adenosine and SOD had no improved effect on survival. Adenosine was the most effective high-energy phosphate in limiting ischemic damage. The free radical scavenger (SOD) was beneficial only at the later stages of ischemia. In this experimental model, there appears to be a role for both phosphates and free radical scavengers in enhancing ischemic tissue survival.
authors.affiliation: Division of Hand Surgery, School of Medicine and Biomedical Sciences, University at Buffalo, State University of New York, USA.
authors.forename: R H
authors.initials: RH
authors.lastname: Ablove
authors.forename: O J
authors.initials: OJ
authors.lastname: Moy
authors.forename: C A
authors.initials: CA
authors.lastname: Peimer
authors.forename: C M
authors.initials: CM
authors.lastname: Severin
authors.forename: F M
authors.initials: FM
authors.lastname: Sherwin
mesh_terms: D000241:Adenosine / Q000494:pharmacology; D000255:Adenosine Triphosphate / Q000494:pharmacology; D000818:Animals; D004305:Dose-Response Relationship, Drug; D016166:Free Radical Scavengers / Q000494:pharmacology*; D005635:Fructosediphosphates / Q000494:pharmacology; D006614:Hindlimb / Q000098:blood supply; D009243:NAD / Q000494:pharmacology; D010755:Organophosphates / Q000494:pharmacology*; D010725:Phosphocreatine / Q000494:pharmacology; D051381:Rats; D017207:Rats, Sprague-Dawley; D015427:Reperfusion Injury / Q000517:prevention & control*; D012092:Replantation; D013482:Superoxide Dismutase / Q000494:pharmacology*
chemical_list: D016166:Free Radical Scavengers; D005635:Fructosediphosphates; D010755:Organophosphates; D010725:Phosphocreatine; D009243:NAD; D000255:Adenosine Triphosphate; D013482:Superoxide Dismutase; D000241:Adenosine; C029063:fructose-1,6-diphosphate