title: Characterization of a spontaneous murine astrocytoma and abrogation of its tumorigenicity by cytokine secretion.
abstract: OBJECTIVE
  The promise of immunotherapies developed against brain tumors in animal models has not been realized in human clinical trials. This may be because of the routine use of rodent tumors artificially induced by chemicals or viruses that do not accurately portray the intrinsic qualities of spontaneously arising human tumors and that often fail to incorporate the role of immunosuppressants, such as transforming growth factor-beta, that are secreted by human gliomas. From an astrocytoma that arose spontaneously in inbred VM/Dk mice, we have characterized a highly tumorigenic spontaneous murine astrocytoma cell line (SMA-560) that retains features of glial differentiation and naturally produces high levels of biologically active transforming growth factor-beta. We have used this model to determine whether cytokine production by tumor cells will inhibit intracerebral astrocytoma growth.
  
  
  METHODS
  Packaging cell lines producing replication-incompetent retroviral vectors were used to transfect the SMA-560 cell line in vitro with the genes encoding the murine cytokines interleukin (IL)-2, IL-3, IL-4, IL-6, tumor necrosis factor-alpha, gamma-interferon, or granulocyte-macrophage colony-stimulating factor or the costimulatory molecule B7.1 (CD80).
  
  
  RESULTS
  Mice challenged intracerebrally with 5000 untransfected SMA-560 cells all succumbed to tumor within 30 days, with a median survival of 25 days. In contrast, mice challenged with SMA-560 cells producing IL-2, IL-4, or tumor necrosis factor-alpha each had a more than 400% increase in median survival (P < 0.0001). In these groups, 78.3% (18 of 23 mice), 66.7% (10 of 15 mice), and 60% (6 of 10 mice) of the mice, respectively, remained alive without evidence of tumor for longer than 100 days after the initial tumor challenge. All other cytokines tested and the expression of B7.1 failed to result in an increase in median survival.
  
  
  CONCLUSIONS
  Using a spontaneous astrocytoma model in an inbred mouse strain, we have shown that cytokine production by glial tumors can abrogate their tumorigenicity in vivo despite production of transforming growth factor-beta. These results predict that approaches directed at cytokine production within intracerebral astrocytomas may be efficacious in human trials and that the "immunological privilege" of the brain may not be absolute under such conditions.
authors.affiliation: Department of Pathology, Duke University, Durham, North Carolina, USA.
authors.forename: J H
authors.initials: JH
authors.lastname: Sampson
authors.forename: D M
authors.initials: DM
authors.lastname: Ashley
authors.forename: G E
authors.initials: GE
authors.lastname: Archer
authors.forename: H E
authors.initials: HE
authors.lastname: Fuchs
authors.forename: G
authors.initials: G
authors.lastname: Dranoff
authors.forename: L P
authors.initials: LP
authors.lastname: Hale
authors.forename: D D
authors.initials: DD
authors.lastname: Bigner
mesh_terms: D000818:Animals; D001254:Astrocytoma / Q000378:metabolism* / Q000473:pathology*; D001932:Brain Neoplasms / Q000378:metabolism* / Q000473:pathology*; D015197:Carcinogenicity Tests; D016207:Cytokines / Q000378:metabolism*; D005260:Female; D005822:Genetic Vectors; D015395:Histocompatibility Antigens Class I / Q000032:analysis; D007150:Immunohistochemistry; D051379:Mice; D008815:Mice, Inbred Strains; D012190:Retroviridae / Q000235:genetics; D014162:Transfection; D016212:Transforming Growth Factor beta / Q000096:biosynthesis; D014407:Tumor Cells, Cultured
chemical_list: D016207:Cytokines; D015395:Histocompatibility Antigens Class I; D016212:Transforming Growth Factor beta