title: Prospective evaluation of protein bound vitamin B12 (cobalamin) malabsorption in the elderly using trout flesh labelled in vivo with 57Co-cobalamin.
abstract: BACKGROUND
  The frequency of dietary protein bound vitamin B12 malabsorption in elderly patients remains controversial.
  
  
  OBJECTIVE
  To evaluate this malabsorption in elderly hospitalised patients using a modified Schilling test.
  
  
  METHODS
  Fourteen elderly patients with low B12 blood levels were prospectively selected from 394 hospitalised patients.
  
  
  METHODS
  The modified Schilling test was performed with trout labelled in vivo.
  
  
  RESULTS
  The test was normal in five healthy elderly subjects, in 7/8 patients with pancreatic insufficiency, and in nine non-elderly patients with antral gastritis. The low decision limit was established at 3.3% (median 4.8%). From the 14 elderly patients with low B12 prospectively selected from 394 hospitalised patients, seven had a real deficiency with anaemia and an increased homocysteine and/or methylmalonate serum level. The modified Schilling test showed malabsorption in five of these patients, including two in which the standard Schilling test was normal, and three in which the standard Schilling test was partially corrected by an intrinsic factor.
  
  
  CONCLUSIONS
  Protein bound vitamin B12 malabsorption was detected in at least 0.5% of elderly hospitalised patients, using the labelled trout flesh absorption test.
authors.affiliation: Laboratory of Cellular and Molecular Pathology in Nutrition, EP CNRS 0616, Faculté de Médecine, Vandoeuvre-lès-Nancy, France.
authors.forename: I
authors.initials: I
authors.lastname: Aimone-Gastin
authors.forename: H
authors.initials: H
authors.lastname: Pierson
authors.forename: C
authors.initials: C
authors.lastname: Jeandel
authors.forename: J P
authors.initials: JP
authors.lastname: Bronowicki
authors.forename: F
authors.initials: F
authors.lastname: Plénat
authors.forename: D
authors.initials: D
authors.lastname: Lambert
authors.forename: F
authors.initials: F
authors.lastname: Nabet-Belleville
authors.forename: J L
authors.initials: JL
authors.lastname: Guéant
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000818:Animals; D003037:Cobalt Radioisotopes; D005069:Evaluation Studies as Topic; D005260:Female; D006760:Hospitalization; D006801:Humans; D008286:Malabsorption Syndromes / Q000175:diagnosis* / Q000209:etiology*; D008297:Male; D008875:Middle Aged; D011446:Prospective Studies; D012546:Schilling Test / Q000379:methods; D014337:Trout; D014806:Vitamin B 12 Deficiency / Q000209:etiology*
chemical_list: D003037:Cobalt Radioisotopes