Expression of nm23-H1 in human meningioma cells.
The expression of nm23-H1 has been demonstrated to be highly correlated with the metastatic potential of various tumors. In the present investigation, meningiomas of different pathological grades were used to study on their nm23-H1 expression. Immunohistochemistry showed that nm23-H1 was expressed mainly in the cytoplasm especially in the perinuclear region in explants under short-term culture. Western-blotting demonstrated the specific expression of nm23 protein in all tumor samples. The expression was also found to be sex-dependent on tumor progression in female, but not in male patients. RT-PCR results confirmed nm23-H1 expression was higher in benign tumors than in their normal counterpart. Our observations thus suggest that nm23-H1 may play an important role in the progression of meningiomas in female patients.
Wang
S W
SW
Department of Anatomy, Chinese University of Hong Kong, NT, Hong Kong.
Yam
H F
HF
Yang
L
L
Ng
H K
HK
Wang
S L
SL
Wu
B Q
BQ
Wang
Z H
ZH
Chew-Cheng
S B
SB
Chew
E C
EC
D000328:Adult; D000368:Aged; D016543:Central Nervous System Neoplasms / Q000235:genetics / Q000378:metabolism*; D005260:Female; D015870:Gene Expression; D006801:Humans; D008297:Male; D008579:Meningioma / Q000235:genetics / Q000378:metabolism*; D008875:Middle Aged; D020559:Monomeric GTP-Binding Proteins; D054778:NM23 Nucleoside Diphosphate Kinases; D009363:Neoplasm Proteins / Q000378:metabolism*; D009701:Nucleoside-Diphosphate Kinase; D012333:RNA, Messenger / Q000235:genetics; D012334:RNA, Neoplasm / Q000235:genetics; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism*
D054778:NM23 Nucleoside Diphosphate Kinases; D009363:Neoplasm Proteins; D012333:RNA, Messenger; D012334:RNA, Neoplasm; D014157:Transcription Factors; C517938:NME1 protein, human; D009701:Nucleoside-Diphosphate Kinase; D020559:Monomeric GTP-Binding Proteins