title: Generation of plasma cells from peripheral blood memory B cells: synergistic effect of interleukin-10 and CD27/CD70 interaction.
abstract: B cells can differentiate into the antibody-secreting cells, plasma cells, whereas the crucial signals that positively control the entry into the pathway to plasma cells have been unclear. Triggering via CD27 by CD27 ligand (CD70) on purified peripheral blood B cells yielded an increase in the number of plasma cells in the presence of interleukin-10 (IL-10). Differentiation into plasma cells by a combination of IL-10 and CD70 transfectants occurred in CD27+ B cells but not in CD27- B cells. Moreover, addition of IL-2 to the IL-10 and CD70-transfect activation system greatly induced differentiation into plasma cells. In the presence of only IL-2, IL-4, or IL-6, CD70 transfectants did not promote differentiation into plasma cells. On the other hand, CD40 signaling increased the expansion of a B-cell pool from peripheral blood B cells primarily activated by IL-2, IL-10, and anti-CD40 monoclonal antibody (MoAb). Finally, CD27 signaling also rescued B cells from IL-10-mediated apoptosis. These data demonstrate that CD27 ligand (CD70) is a key molecule to prevent the IL-10-mediated promotion of apoptosis and to direct the differentiation of CD27+ memory B cells toward plasma cells in cooperation with IL-10.
authors.affiliation: Department of Pediatrics and Blood Transfusion, Shinshu University School of Medicine, Matsumoto, Japan.
authors.forename: K
authors.initials: K
authors.lastname: Agematsu
authors.forename: H
authors.initials: H
authors.lastname: Nagumo
authors.forename: Y
authors.initials: Y
authors.lastname: Oguchi
authors.forename: T
authors.initials: T
authors.lastname: Nakazawa
authors.forename: K
authors.initials: K
authors.lastname: Fukushima
authors.forename: K
authors.initials: K
authors.lastname: Yasui
authors.forename: S
authors.initials: S
authors.lastname: Ito
authors.forename: T
authors.initials: T
authors.lastname: Kobata
authors.forename: C
authors.initials: C
authors.lastname: Morimoto
authors.forename: A
authors.initials: A
authors.lastname: Komiyama
mesh_terms: D000328:Adult; D015703:Antigens, CD; D017209:Apoptosis / Q000502:physiology; D016175:B-Lymphocyte Subsets / Q000166:cytology*; D053282:CD27 Ligand; D023201:CD40 Ligand; D002454:Cell Differentiation; D002478:Cells, Cultured; D006801:Humans; D007156:Immunologic Memory; D016753:Interleukin-10 / Q000235:genetics / Q000502:physiology*; D007376:Interleukin-2 / Q000494:pharmacology; D015847:Interleukin-4 / Q000494:pharmacology; D008562:Membrane Glycoproteins / Q000235:genetics / Q000502:physiology; D008565:Membrane Proteins / Q000235:genetics / Q000502:physiology*; D010950:Plasma Cells / Q000166:cytology*; D011993:Recombinant Fusion Proteins / Q000502:physiology; D015398:Signal Transduction / Q000502:physiology*; D014162:Transfection; D018127:Tumor Necrosis Factor Receptor Superfamily, Member 7 / Q000235:genetics / Q000502:physiology*
chemical_list: D015703:Antigens, CD; D053282:CD27 Ligand; C505771:CD70 protein, human; D007376:Interleukin-2; D008562:Membrane Glycoproteins; D008565:Membrane Proteins; D011993:Recombinant Fusion Proteins; D018127:Tumor Necrosis Factor Receptor Superfamily, Member 7; D016753:Interleukin-10; D023201:CD40 Ligand; D015847:Interleukin-4