Identification of a differential display product associated with apoptosis in chicken thymocytes.
To further elucidate the cellular mechanisms that mediate programmed cell death in avian immune cells, differential display analysis was employed to identify differentially expressed genes in chicken thymocytes undergoing apoptosis. Primary cultures of thymocytes were treated with dexamethasone to activate apoptosis and RNA was isolated for differential display analysis. A differential display product designated A1 (479 bp) was identified. This display product was subcloned and induced expression of the genes was confirmed by ribonuclease protection analysis. Nucleotide sequence analysis of A1 revealed a putative 82 amino acid open reading frame that demonstrated limited homology with Bad, an apoptotic regulatory protein. Thus, A1 may represent the avian homolog of Bad.
Thomson
J M
JM
Department of Poultry Science, University of Georgia, Athens 30602-2772, USA.
Waldrip
H W
HW
Compton
M M
MM
D000595:Amino Acid Sequence; D000818:Animals; D017209:Apoptosis / Q000235:genetics*; D001483:Base Sequence; D002478:Cells, Cultured; D002645:Chickens; D018076:DNA, Complementary; D005786:Gene Expression Regulation; D006801:Humans; D008969:Molecular Sequence Data; D012260:Ribonucleases / Q000378:metabolism; D013950:Thymus Gland / Q000166:cytology
D018076:DNA, Complementary; D012260:Ribonucleases