Validated assay for the quantification of anastrozole in human plasma by capillary gas chromatography-63Ni electron capture detection.
An assay was developed for the quantification of anastrozole [2,2'-[5-(1H-1,2,4-triazol-1-ymethyl)-1,3-phenylene]bis(2-++ +methylpropiononitrile)] in human plasma using liquid-liquid extraction. Anastrozole and an internal standard were chromatographed and detected by gas chromatography with electron capture detection, using a combination temperature-pressure program. The range of the assay is 3 to 100 ng/ml. Anastrozole was quantified by comparing its peak area to that of an internal standard. A cross-validation of this assay was also successfully performed between several laboratories.
Bock
M J
MJ
Zeneca Pharmaceuticals, Wilmington, DE 19850-5437, USA.
Bara
I
I
LeDonne
N
N
Martz
A
A
Dyroff
M
M
D000077384:Anastrozole; D018931:Antineoplastic Agents, Hormonal / Q000097:blood* / Q000493:pharmacokinetics; D002849:Chromatography, Gas / Q000295:instrumentation; D004355:Drug Stability; D005260:Female; D006801:Humans; D009570:Nitriles / Q000097:blood* / Q000493:pharmacokinetics; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D014230:Triazoles / Q000097:blood* / Q000493:pharmacokinetics
D018931:Antineoplastic Agents, Hormonal; D009570:Nitriles; D014230:Triazoles; D000077384:Anastrozole