Purification and gas chromatographic-mass spectrometric characterization of non-methylene interrupted fatty acid incorporated in rat liver.
A C20 non-methylene interrupted trienoic acid detected in the liver of rat fed with a pine (Pinus koraiensis) seed oil diet was purified by two-step argentation thin-layer chromatography (AgTLC) and characterized by gas chromatography-mass spectrometry (GC-MS). First, a C20 methyl trienoate fraction was obtained from fatty acid methyl esters prepared from rat liver by 5% AgTLC developed with petroleum ether-diethyl ether-acetic acid (70:20:2, v/v) as a solvent system. The fraction was then subjected to AgTLC developed with benzene-acetone-diethyl ether-acetic acid (65:15:15:5, v/v) which could separate non-methylene interrupted fatty acids (NMIFA) from usual MIFAs. The purified C20 NMIFA was partially hydrogenated, and the resulting three kinds of the C20 monoenoate were analyzed by GC-MS after conversion to their dimethyl disulfide (DMDS) adducts. The results revealed that the original C20 non-methylene interrupted trienoic acid detected in the liver of rats fed with a pine seed oil diet was delta-5,11,14/20:3, a minor component of pine seed oil.
Tanaka
T
T
Department of Food Science and Technology, Fukuyama University, Japan.
Shibata
K
K
Hino
H
H
Murashita
T
T
Kayama
M
M
Satouchi
K
K
D000818:Animals; D002855:Chromatography, Thin Layer; D004041:Dietary Fats / Q000008:administration & dosage; D005231:Fatty Acids, Unsaturated / Q000737:chemistry / Q000302:isolation & purification*; D008401:Gas Chromatography-Mass Spectrometry; D008099:Liver / Q000737:chemistry* / Q000378:metabolism; D008297:Male; D010743:Phospholipids / Q000737:chemistry; D010938:Plant Oils / Q000008:administration & dosage; D051381:Rats; D017207:Rats, Sprague-Dawley; D012639:Seeds / Q000737:chemistry
D004041:Dietary Fats; D005231:Fatty Acids, Unsaturated; D010743:Phospholipids; D010938:Plant Oils