title: Immunohistochemical and structural characteristics of the reticular framework of the white pulp and marginal zone in the human spleen.
abstract: The reticular framework of the white pulp (WP) and marginal zone (MZ) consists of reticulum cells and reticulin fibers. The antigenic heterogeneity of the reticular framework is well documented in the mouse and rat spleen. The aim of the present study is to characterize the reticular framework of the WP and MZ of the human spleen.
  
  
  
  Nine surgically resected human spleens were investigated. Five of the nine spleens were perfused. Formalin-fixed materials were embedded in paraffin and serial sections prepared for hematoxylin-eosin, silver staining, and immunohistochemical examination. Electron and immuno-electron microscopy were also applied. Using confocal laser scanning microscopy, the reticular framework was analyzed three-dimensionally.
  
  
  
  The reticulin fibers of the framework were immunostained for type IV collagen in the WP and MZ. The WP was three-dimensionally delimited by the alpha-smooth muscle actin (alpha-SMA)-positive reticulum cells. In the WP, the distribution of alpha-SMA-positive reticulum cells formed the reticular framework of the periarteriolar lymphoid sheath (PALS). They also ensheathed the reticulin fibers. Interdigitating cells (IDCs) were scattered throughout the framework. A few IDCs attached to the framework. In the lymph follicle (LF), reticulum cells were not alpha-SMA-positive. The mesh of follicular dendritic cells (FDCs) was found in the germinal center. In places, the reticulin fibers were involved in the mesh of the FDCs and covered by the cytoplasm of FDCs. In the MZ, alpha-SMA-positive reticulum cells were arranged in a mesh pattern and ensheathed the fine reticulin fibers.
  
  
  
  The reticular framework of the PALS, LF, and MZ is specialized into heterogeneous components in the human spleen. The heterogeneity of the framework may induce the segregation of T and B lymphocytes.
authors.affiliation: Department of Pathology, School of Medicine, Iwate Medical University, Morioka, Japan. tsatoh@iwate-med.ac.jp
authors.forename: T
authors.initials: T
authors.lastname: Satoh
authors.forename: R
authors.initials: R
authors.lastname: Takeda
authors.forename: H
authors.initials: H
authors.lastname: Oikawa
authors.forename: R
authors.initials: R
authors.lastname: Satodate
mesh_terms: D000199:Actins / Q000032:analysis; D000328:Adult; D000368:Aged; D001160:Arterioles / Q000737:chemistry / Q000648:ultrastructure; D002675:Child, Preschool; D003094:Collagen / Q000032:analysis; D003713:Dendritic Cells / Q000737:chemistry / Q000648:ultrastructure; D005260:Female; D006801:Humans; D007150:Immunohistochemistry / Q000379:methods; D008297:Male; D018613:Microscopy, Confocal; D016253:Microscopy, Immunoelectron; D008875:Middle Aged; D012155:Reticulin / Q000737:chemistry / Q000648:ultrastructure; D013154:Spleen / Q000098:blood supply / Q000737:chemistry / Q000166:cytology*
chemical_list: D000199:Actins; D012155:Reticulin; D003094:Collagen