title: Hemagglutination test for the diagnosis of human neurocysticercosis: development of a stable reagent using homologous and heterologous antigens.
abstract: A hemagglutination (HA) test was standardized using formalin- and tannin-treated gander red blood cells sensitized with a total salt extract of C. cellulosae (HA-Cc) and an antigenic extract of Cysticercus longicollis (HA-Cl) vesicular fluid. A total of 61 cerebrospinal fluid (CSF) samples were assayed, 41 from patients with neurocysticercosis and 20 from a control group which were, respectively, reactive and non-reactive to ELISA using C. cellulosae. The CSF samples from the control group did not react and 35 (85.4%) and 34 (82.9%) CSF samples from patients were reactive to the HA-Cc and HA-Cl tests, respectively. The reagents ready for use were stable up to 6 months when stored at 4 degrees C in 50% glycerol. The present results confirm that the reagent using Cysticercus longicollis stabilized with glycerol can be used as an alternative in the immunological diagnosis of neurocysticercosis.
authors.affiliation: Faculty of Pharmaceutical Sciences, University of São Paulo, Brasil.
authors.forename: A P
authors.initials: AP
authors.lastname: Ferreira
authors.forename: A J
authors.initials: AJ
authors.lastname: Vaz
authors.forename: P M
authors.initials: PM
authors.lastname: Nakamura
authors.forename: A T
authors.initials: AT
authors.lastname: Sasaki
authors.forename: A W
authors.initials: AW
authors.lastname: Ferreira
authors.forename: J A
authors.initials: JA
authors.lastname: Livramento
mesh_terms: D000818:Animals; D000947:Antigens, Helminth / Q000276:immunology; D003551:Cysticercosis / Q000134:cerebrospinal fluid / Q000175:diagnosis* / Q000276:immunology; D005260:Female; D006386:Hemagglutination Tests / Q000379:methods*; D006801:Humans; D051379:Mice; D008807:Mice, Inbred BALB C
chemical_list: D000947:Antigens, Helminth