title: Overexpression of phospholipase C-gamma1 in rat 3Y1 fibroblast cells leads to malignant transformation.
abstract: Phospholipase C-gamma1 (PLC-gamma1) mediates signals from various extracellular origins to evoke cellular events such as mitogenesis. Previously, we reported that PLC-gamma1 was highly expressed in colorectal cancer and familial adenomatous polyposis, suggesting that PLC-gamma1 might be oncogenic. In this study, we have established rat 3Y1 fibroblasts that overexpress whole PLC-gamma1 and src homology 2 (SH2)-SH2-SH3 domain of PLC-gamma1. These cells showed a transformed phenotype and were tumorigenic when transplanted into nude mice. These results indicate that overexpression of PLC-gamma1 could transform rat fibroblasts, and the transformation is mediated by SH2-SH2-SH3 domain of PLC-gamma1.
authors.affiliation: Department of Biology, Daejin University, Pochongun, Kyunggido.
authors.forename: J S
authors.initials: JS
authors.lastname: Chang
authors.forename: D Y
authors.initials: DY
authors.lastname: Noh
authors.forename: I A
authors.initials: IA
authors.lastname: Park
authors.forename: M J
authors.initials: MJ
authors.lastname: Kim
authors.forename: H
authors.initials: H
authors.lastname: Song
authors.forename: S H
authors.initials: SH
authors.lastname: Ryu
authors.forename: P G
authors.initials: PG
authors.lastname: Suh
mesh_terms: D000818:Animals; D002471:Cell Transformation, Neoplastic; D002478:Cells, Cultured; D005347:Fibroblasts / Q000166:cytology* / Q000201:enzymology*; D007527:Isoenzymes / Q000096:biosynthesis / Q000502:physiology*; D051379:Mice; D008819:Mice, Nude; D051966:Phospholipase C gamma; D051381:Rats; D014162:Transfection; D010738:Type C Phospholipases / Q000096:biosynthesis / Q000502:physiology*; D018909:src Homology Domains
chemical_list: D007527:Isoenzymes; D010738:Type C Phospholipases; D051966:Phospholipase C gamma