Effect of selenium in recovery of immunity damaged by H2O2 and 60Co radiation.
Con A stimulated lymphocytes proliferation was measured as [3H]thymidine incorporation and IgG was quantified by single radial immunodiffusion to study recovering or protecting effect of selenium (Se) on immunity attacked by exogenous active oxygen species, H2O2 and 60Co-radiation, respectively. Lipid peroxidation was also determined to observe the relation between antioxidation ability and protecting ability of Se. It was found that H2O2 injured lymphocytes immunocompetence deeply and 60Co-radiation decreased immune response capacity greatly, but that administration of Se counteracts this damage. The antioxidative ability of Se was correlated with its protecting ability.
Sun
E
E
Department of Chemistry, Huazhong University of Science and Technology, Wuhan, People's Republic of China.
Xu
H
H
Liu
Q
Q
Zhou
J
J
Zuo
P
P
Wang
J
J
D000818:Animals; D000917:Antibody Formation / Q000187:drug effects / Q000528:radiation effects; D000975:Antioxidants / Q000494:pharmacology*; D003037:Cobalt Radioisotopes; D003208:Concanavalin A / Q000494:pharmacology; D005720:Gamma Rays; D006861:Hydrogen Peroxide / Q000037:antagonists & inhibitors* / Q000633:toxicity*; D007109:Immunity / Q000187:drug effects* / Q000528:radiation effects*; D007111:Immunity, Cellular / Q000187:drug effects / Q000528:radiation effects; D008054:Lipid Peroxides / Q000378:metabolism; D051379:Mice; D016877:Oxidants / Q000276:immunology; D017382:Reactive Oxygen Species / Q000276:immunology; D012643:Selenium / Q000494:pharmacology*
D000975:Antioxidants; D003037:Cobalt Radioisotopes; D008054:Lipid Peroxides; D016877:Oxidants; D017382:Reactive Oxygen Species; D003208:Concanavalin A; D006861:Hydrogen Peroxide; D012643:Selenium