Differential splicing and alternative polyadenylation generate multiple mimecan mRNA transcripts.
We previously showed the 25-kDa corneal keratan sulfate proteoglycan to be a translation product of the gene producing osteoglycin and proposed the name mimecan for this gene and its product. We also demonstrated three mimecan RNA transcripts using Northern blot analysis. In this report, we investigate the mechanisms accounting for these transcripts. Ribonuclease protection analysis and reverse transcription-polymerase chain reaction of bovine corneal mRNA detected a mimecan transcript that lacked 278 base pairs of the 5'-untranslated region between residues 62 and 340. This splice variant represents the predominant form of mimecan mRNA in bovine cornea and sclera. It was also detectable in other bovine tissues as a minor transcript. Two additional cDNA clones that were isolated contained 398 bases of nucleotide sequence at the 3'-end of mimecan cDNA, not present in the published sequence. Ribonuclease protection analyses with the 3'-probe, which included the new sequence, allow detection of three RNA transcripts while 5'-probes recognized only two. These results indicate that the three canonical polyadenylation sites in the 3'-untranslated region of mimican mRNA are alternatively selected. Possible roles for this previously undetected degree of diversity of mimecan RNA isoforms transcribed in the same tissue are discussed.
Tasheva
E S
ES
Division of Biology, Kansas State University, Manhattan, Kansas 66506-4901, USA. est@ksu.edu
Corpuz
L M
LM
Funderburgh
J L
JL
Conrad
G W
GW
D000818:Animals; D002417:Cattle; D003001:Cloning, Molecular; D018076:DNA, Complementary; D006023:Glycoproteins / Q000235:genetics*; D006133:Growth Substances / Q000235:genetics*; D011061:Poly A / Q000378:metabolism*; D012326:RNA Splicing; D012333:RNA, Messenger / Q000235:genetics*
D018076:DNA, Complementary; D006023:Glycoproteins; D006133:Growth Substances; D012333:RNA, Messenger; D011061:Poly A