title: M. leprae binds to a 28-30-kDa phosphorylated glycoprotein of rat peripheral nerve.
abstract: To understand Mycobacterium leprae-peripheral nerve interaction, we have investigated the binding of M. leprae to rat peripheral nerve proteins in an in vitro model using 32P-phosphorylated proteins of the peripheral nerve. Intact M. leprae binds to a major phosphorylated protein of 28-30 kDa and, to a minor extent, to a few proteins of molecular weight 45-55 kDa. This binding was more specific for M. leprae since only insignificant binding was observed with other bacteria, such as M. bovis or Escherichia coli. M. leprae did not show binding to several phosphorylated proteins of the rat brain. The 28-30-kDa binding protein of the rat peripheral nerve was found to be a glycoprotein by concanavalin A-Sepharose column chromatography.
authors.affiliation: Neurochemistry Laboratory, Christian Medical College Hospital, Vellore, Tamil Nadu, India.
authors.forename: L M
authors.initials: LM
authors.lastname: Suneetha
authors.forename: P R
authors.initials: PR
authors.lastname: Satish
authors.forename: S
authors.initials: S
authors.lastname: Suneetha
authors.forename: C K
authors.initials: CK
authors.lastname: Job
authors.forename: A S
authors.initials: AS
authors.lastname: Balasubramanian
mesh_terms: D000818:Animals; D008970:Molecular Weight; D009166:Mycobacterium leprae / Q000378:metabolism*; D009419:Nerve Tissue Proteins / Q000737:chemistry / Q000378:metabolism*; D010525:Peripheral Nerves / Q000737:chemistry*; D010750:Phosphoproteins / Q000737:chemistry / Q000378:metabolism*; D011485:Protein Binding; D051381:Rats
chemical_list: D009419:Nerve Tissue Proteins; D010750:Phosphoproteins