Intracytoplasmic localization of antigenic heat-stable 120- to 130-kilodalton proteins (PS120) common to spotted fever group rickettsiae demonstrated by immunoelectron microscopy.
Immunoelectron microscopy demonstrated antigenic heat-stable 120- to 130-kilodalton proteins (PS120) of spotted fever group (SFG) rickettsiae with antiserum against recombinant PS120 of Rickettsia japonica. In the case of R. japonica, a major part of the protein was shown to be localized outside the electron-lucent nucleoid-like region in the cytoplasm of the organisms. The other SFG rickettsiae represented a similar localization of the PS120 antigens cross-reactive to that of R. japonica. On the other hand, a typhus group rickettsia demonstrated no antigens cross-reactive to the PS120 of SFG rickettsiae.
Uchiyama
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Department of Virology, School of Medicine, The University of Tokushima, Japan. uchiyama@basic.med.tokushima-u.ac.jp
D000818:Animals; D000907:Antibodies, Bacterial / Q000276:immunology; D000942:Antigens, Bacterial / Q000032:analysis* / Q000737:chemistry / Q000276:immunology; D001426:Bacterial Proteins / Q000032:analysis* / Q000737:chemistry / Q000276:immunology; D002522:Chlorocebus aethiops; D003429:Cross Reactions; D006358:Hot Temperature; D007150:Immunohistochemistry / Q000379:methods; D016253:Microscopy, Immunoelectron; D011994:Recombinant Proteins / Q000276:immunology; D012281:Rickettsia / Q000276:immunology*; D014709:Vero Cells
D000907:Antibodies, Bacterial; D000942:Antigens, Bacterial; D001426:Bacterial Proteins; D011994:Recombinant Proteins