Bidirectional electrical coupling between inspiratory motoneurons in the newborn mouse nucleus ambiguus.
Some spinal and brain stem motoneurons are electrically coupled in the early postnatal period. To test whether respiratory motoneurons in the brain stem are electrically coupled, we performed single and dual whole cell patch recordings from presumptive motoneurons in the nucleus ambiguus in a rhythmically active brain stem slice from newborn mice. Two of eight (25%) biocytin-injected neurons showed dye-coupling and 4 of 11 (36%) of intracellularly recorded pairs of neurons showed evidence of bidirectional electrical coupling. Impulse activity in one cell elicited small spikelets in the other and hyperpolarization of one cell led to hyperpolarization of the other with a coupling ratio (DeltaV2:DeltaV1) of 0.03-0.14. We conclude that inspiratory ambiguus motoneurons in the newborn mouse brain stem are bidirectionally electrically coupled, which may serve to transmit or coordinate signals, chemical or electrical.
Rekling
J C
JC
Department of Physiological Science, Systems Neurobiology Laboratory, University of California, Los Angeles, California 90095-1527, USA.
Feldman
J L
JL
D000818:Animals; D000831:Animals, Newborn; D001933:Brain Stem / Q000166:cytology / Q000502:physiology*; D019706:Excitatory Postsynaptic Potentials; D017629:Gap Junctions / Q000502:physiology*; D008564:Membrane Potentials / Q000502:physiology; D051379:Mice; D008807:Mice, Inbred BALB C; D009046:Motor Neurons / Q000502:physiology*; D018408:Patch-Clamp Techniques; D010614:Pharynx / Q000294:innervation*; D012119:Respiration / Q000502:physiology*