Wig-1, a new p53-induced gene encoding a zinc finger protein.
Wild type p53 expressed from a temperature-sensitive (ts p53) construct induces both G1 cell cycle arrest and apoptosis in the p53-negative J3D mouse T lymphoma line (Wang et al., 1995). Using differential display analysis, we have identified one new p53-induced gene, wig-1 (for wild type p53-induced gene 1), whose 7.6 kb and 2.2 kb transcripts are upregulated in ts p53-transfected J3D cells following induction of wild type p53 expression by temperature shift to 32 degrees C. The wig-1 transcripts were also induced in irradiated NIH3T3 and p21-/- fibroblasts but not in irradiated p53-/- fibroblasts. Whole body gamma irradiation caused induction of both wig-1 transcripts in mouse brain, testis, kidney, spleen and lung. A basal wig-1 expression was detected in brain, testis and kidney. The WIG-1 protein contains three zinc finger motifs and a putative nuclear localization signal.
Varmeh-Ziaie
S
S
Microbiology and Tumor Biology Center, Karolinska Institute, Stockholm, Sweden.
Okan
I
I
Wang
Y
Y
Magnusson
K P
KP
Warthoe
P
P
Strauss
M
M
Wiman
K G
KG
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D015152:Blotting, Northern; D003001:Cloning, Molecular; D018076:DNA, Complementary; D004268:DNA-Binding Proteins / Q000235:genetics* / Q000378:metabolism*; D005720:Gamma Rays; D008223:Lymphoma / Q000235:genetics; D051379:Mice; D008969:Molecular Sequence Data; D009154:Mutation; D009687:Nuclear Proteins; D016133:Polymerase Chain Reaction / Q000379:methods; D016601:RNA-Binding Proteins; D017386:Sequence Homology, Amino Acid; D013696:Temperature; D014018:Tissue Distribution; D014407:Tumor Cells, Cultured; D016159:Tumor Suppressor Protein p53 / Q000378:metabolism*; D014916:Whole-Body Irradiation; D016335:Zinc Fingers / Q000235:genetics* / Q000528:radiation effects
D018076:DNA, Complementary; D004268:DNA-Binding Proteins; D009687:Nuclear Proteins; D016601:RNA-Binding Proteins; D016159:Tumor Suppressor Protein p53; C491518:Wig1 protein, mouse