title: Involvement of integrins and the cytoskeleton in modulation of skeletal muscle glycogen synthesis by changes in cell volume.
abstract: Muscle glycogen synthesis is modulated by physiologically relevant changes in cell volume. We have investigated the possible involvement of integrin-extracellular matrix interactions in this process using primary cultures of rat skeletal muscle subject to hypo- or hyper-osmotic exposure with integrin binding peptide GRGDTP to disrupt integrin actions and the inactive analogue GRGESP as control. Osmotically induced increases (77%) and decreases (34%) in glycogen synthesis (D-[14C]glucose incorporation into glycogen) were prevented by GRGDTP (but not GRGESP) without affecting glucose transport. Cytoskeletal disruption with cytochalasin D or colchicine had similar effects to GRGDTP. Osmotically induced modulation of muscle glycogen synthesis involves integrin-extracellular matrix interactions and cytoskeletal elements, possibly as components of a cell-volume 'sensing' mechanism.
authors.affiliation: Department of Anatomy and Physiology, University of Dundee, UK. s.y.low@dundee.ac.uk
authors.forename: S Y
authors.initials: SY
authors.lastname: Low
authors.forename: M J
authors.initials: MJ
authors.lastname: Rennie
authors.forename: P M
authors.initials: PM
authors.lastname: Taylor
mesh_terms: D000730:Androstadienes / Q000494:pharmacology; D000818:Animals; D002478:Cells, Cultured; D003078:Colchicine / Q000494:pharmacology; D003470:Culture Media; D015638:Cytochalasin D / Q000494:pharmacology; D003599:Cytoskeleton / Q000378:metabolism*; D003847:Deoxyglucose / Q000378:metabolism; D005947:Glucose / Q000378:metabolism; D006003:Glycogen / Q000096:biosynthesis*; D016023:Integrins / Q000378:metabolism*; D009004:Monosaccharide Transport Proteins / Q000378:metabolism; D018482:Muscle, Skeletal / Q000166:cytology / Q000378:metabolism*; D009842:Oligopeptides / Q000378:metabolism / Q000494:pharmacology; D009997:Osmotic Pressure; D051381:Rats; D000077191:Wortmannin
chemical_list: D000730:Androstadienes; D003470:Culture Media; D016023:Integrins; D009004:Monosaccharide Transport Proteins; D009842:Oligopeptides; C080493:fibronectin attachment peptide; D015638:Cytochalasin D; D006003:Glycogen; D003847:Deoxyglucose; D005947:Glucose; D003078:Colchicine; D000077191:Wortmannin