Effect of infection brain edema on NMDA receptor binding in rat brain in vivo.
The purpose of this study was to determine the effect of infection brain edema (IBE) in the rat model induced by injecting pertussis bacilli (PB) into the left carotid artery. The specific binding of N-methyl-D-aspartate (NMDA) receptor with [3H] MK-801 was measured in the neuronal membrane of cerebral cortex. The Scatchard plots were performed. The Bmax values were 0.623 +/- 0.082 and 0.606 +/- 0.087 pmol/mg protein in the group that received normal saline (NS) and PB respectively (P < 0.05). The Kd values were 43.1 +/- 4.2 and 30.5 +/- 3.0 nM in the groups NS and PB respectively. The results indicated that the affinity of NMDA receptor was significantly higher in the group PB than group NS, whereas the total number of NMDA receptors had not changed in the IBE model. The increase of affinity of NMDA receptor can be blockaded by MK-801 pretreatment in vivo.
Cheng
G S
GS
Central Laboratory, Hunan Medical University, Changsha, Peoples Republic of China.
Chen
J F
JF
Chen
X
X
Chen
L H
LH
Tao
Y G
YG
Yang
Y J
YJ
D000818:Animals; D001886:Bordetella pertussis; D001921:Brain / Q000378:metabolism*; D001929:Brain Edema / Q000378:metabolism* / Q000382:microbiology; D004195:Disease Models, Animal; D008297:Male; D011869:Radioligand Assay; D051381:Rats; D017207:Rats, Sprague-Dawley; D016194:Receptors, N-Methyl-D-Aspartate / Q000378:metabolism*; D014917:Whooping Cough / Q000150:complications / Q000378:metabolism*
D016194:Receptors, N-Methyl-D-Aspartate