title: Localization of ROMK channels in the rat kidney.
abstract: Renal potassium secretion occurs in the distal segments of the nephron through apically located secretory potassium (SK) channels. SK may correspond to the ROMK channels cloned from rat kidney. In this study, the localization of ROMK at the cellular level in the rat kidney was examined using an affinity-purified polyclonal antibody raised against a C-terminal peptide of ROMK. The specificity of the antibody was demonstrated by immunoblots of membranes of Xenopus oocytes expressing ROMK2. Immunoblots of homogenates from rat renal outer medulla and cortex revealed predominant bands of 70 to 75 kD, which were ablated by preadsorption with an excess of peptide. These bands were specific for the rat kidney. Immunolocalization studies revealed that ROMK is expressed in specific nephron segments in both the cortex and medulla. In the cortex, ROMK was found in the apical domain of the thick ascending limb of Henle's loop, the connecting tubule, and in some, but not all, cells of cortical collecting tubules. In the medulla, expression in the apical membrane of the thick ascending limbs of Henle's loop was strong, whereas outer medullary collecting ducts were weakly stained. Expression in the thick ascending limb was also heterogeneous; some cells that expressed the Na-K-Cl cotransporter were weakly stained with the anti-ROMK antibody. No staining of glomeruli, proximal tubules, or inner medullary collecting ducts was found. The localization of ROMK agrees well with the findings of SK in patch-clamp studies and supports the view that ROMK is the SK channel of the distal segments of the nephron.
authors.affiliation: Department of Physiology and Biophysics, Cornell University Medical College, New York, New York 10021, USA.
authors.forename: P A
authors.initials: PA
authors.lastname: Mennitt
authors.forename: J B
authors.initials: JB
authors.lastname: Wade
authors.forename: C A
authors.initials: CA
authors.lastname: Ecelbarger
authors.forename: L G
authors.initials: LG
authors.lastname: Palmer
authors.forename: G
authors.initials: G
authors.lastname: Frindt
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D016764:Cell Polarity; D019084:Fluorescent Antibody Technique, Indirect; D007668:Kidney / Q000737:chemistry* / Q000648:ultrastructure; D007672:Kidney Cortex / Q000737:chemistry / Q000648:ultrastructure; D007679:Kidney Medulla / Q000737:chemistry / Q000648:ultrastructure; D007685:Kidney Tubules, Collecting / Q000737:chemistry; D008138:Loop of Henle / Q000737:chemistry / Q000648:ultrastructure; D008969:Molecular Sequence Data; D009399:Nephrons / Q000737:chemistry; D011188:Potassium / Q000378:metabolism; D015221:Potassium Channels / Q000032:analysis*; D024661:Potassium Channels, Inwardly Rectifying; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: C510692:Kcnj1 protein, rat; D015221:Potassium Channels; D024661:Potassium Channels, Inwardly Rectifying; D011188:Potassium