Detection of chromosomal aberrations in seminomatous germ cell tumours using comparative genomic hybridization.
Comparative genomic hybridization (CGH) was used to evaluate tissue specimens from 16 seminomas in order to elucidate the pathogenesis of germ cell tumours in males. A characteristic pattern of losses and gains within the entire genomes was detected in 94% of the seminomas by comparing the ratio profiles of the tumours with a standard of cytogenetically normal genomic DNA. Losses represented 43% of the total number of alterations often affecting chromosomes and chromosome arms 4, 5, 11, 13q, and 18q. Gains amounted to 57% and were often observed on 1q, 7, 8, 12, 14q, 15q, 21q, and 22q. Aberrations of 12p and 21q appeared most consistently. Results from CGH analysis displayed no relationship to the clinical stages of the malignancy. Some rare aberrations appeared, however, only in clinical stage II and in tumours showing relapse in the contralateral testis following orchiectomy, although the alterations were not present in all of the tumours in question. Losses of 16q13-21 and gains of 9q22.1-22.2 were demonstrated in both groups, while loss of 16p12 and gains of 6p21 and 6q23.3-24 were detected in the latter group as well. In conclusion, a specific pattern of chromosomal alterations was demonstrated in the seminomas by improved detection criteria, which increased specificity and sensitivity. The rare aberrations, which appeared only in tumours in improved detection criteria, which increased specificity and sensitivity. The rare aberrations, which appeared only in tumours in clinical stage II and relapsed tumours, may be linked to tumour progression, invasiveness, and bilateral disease.
Ottesen
A M
AM
Department of Growth and Reproduction, Juliane Marie Centre, National University Hospital, Copenhagen, Denmark.
Kirchhoff
M
M
Rajpert De-Meyts
E
E
Maahr
J
J
Gerdes
T
T
Rose
H
H
Lundsteen
C
C
Petersen
P M
PM
Philip
J
J
Skakkebaek
N E
NE
D002869:Chromosome Aberrations; D002871:Chromosome Banding; D002872:Chromosome Deletion; D004273:DNA, Neoplasm / Q000032:analysis; D052138:Genes, Neoplasm; D006801:Humans; D007621:Karyotyping; D008297:Male; D009367:Neoplasm Staging; D009693:Nucleic Acid Hybridization; D018239:Seminoma / Q000235:genetics* / Q000473:pathology; D013736:Testicular Neoplasms / Q000235:genetics* / Q000473:pathology
D004273:DNA, Neoplasm