The role of complement, antibody, and tumor necrosis factor alpha in the killing of Plasmodium falciparum by the monocytic cell line THP-1.
Killing of Plasmodium falciparum blood forms by the differentiated human myelomonocytic THP-1Mo cell line was studied by a radiometric assay. Results showed that parasite killing was promoted by complement, antimalarial antibody, and the cytokines tumor necrosis factor alpha and gamma interferon. Differentiated THP-1Mo appears to be a useful monocytic cell line for the study of mechanisms of immunity to Plasmodium.
Kumaratilake
L M
LM
Department of Immunology and University of Adelaide Department of Paediatrics, Women's and Children's Hospital, North Adelaide, South Australia. lkumarat@adelaide.medicine.edu.au
Ferrante
A
A
Jaeger
T
T
Morris-Jones
S D
SD
D000818:Animals; D000913:Antibodies, Protozoan / Q000276:immunology*; D002460:Cell Line; D003165:Complement System Proteins / Q000276:immunology*; D003602:Cytotoxicity, Immunologic; D006801:Humans; D016778:Malaria, Falciparum / Q000276:immunology*; D009000:Monocytes / Q000276:immunology* / Q000469:parasitology*; D010963:Plasmodium falciparum; D014409:Tumor Necrosis Factor-alpha / Q000276:immunology*
D000913:Antibodies, Protozoan; D014409:Tumor Necrosis Factor-alpha; D003165:Complement System Proteins