Cloning and nucleotide sequence analysis of a gene encoding an OXA-derived beta-lactamase in Acinetobacter baumannii.
A clinical strain of Acinetobacter baumannii (strain Ab41) that was resistant to all beta-lactam antibiotics tested except ceftazidime, ceftriaxone, ceftizoxime, and imipenem produced three beta-lactamases: a presumptive chromosomal cephalosporinase, a TEM-1-like beta-lactamase (pI 5.4), and a novel OXA-derived beta-lactamase named OXA-21 (pI 7.0). The gene encoding OXA-21 was located in an integron. The nucleotide sequence showed three mutations compared with the sequence of OXA-3, with two being silent; the nonsilent mutation generated a substitution of Ile-217 to Met.
Vila
J
J
Departament de Microbiologia, Hospital Clínic, Facultat de Medicina, Universitat de Barcelona, Spain. vila@microclinic.med.ub.es
Navia
M
M
Ruiz
J
J
Casals
C
C
D000150:Acinetobacter / Q000201:enzymology* / Q000235:genetics*; D000595:Amino Acid Sequence; D001483:Base Sequence; D003001:Cloning, Molecular; D007525:Isoelectric Focusing; D008969:Molecular Sequence Data; D017422:Sequence Analysis, DNA; D001618:beta-Lactamases / Q000235:genetics*
C039404:beta-lactamase OXA-2; D001618:beta-Lactamases