title: Effect of cytochalasin B and cycloheximide on the activation rate, chromosome constituent and in vitro development of porcine oocytes following parthenogenetic stimulation.
abstract: Activation rate, chromosome constituent and developmental pattern of porcine oocytes was examined in the presence and absence of cytochalasin B and cycloheximide following parthenogenetic stimulation. Treatment with cycloheximide after ethanol or Ca2+ ionophore treatment increased the incidence of activation. The percentage of oocytes with two or more female pronuclei was higher (P < 0.05) in oocytes treated with cytochalasin B than in control or cycloheximide-treated oocytes. Treatment with both electrical stimulation and cytochalasin B increased the incidence of diploid chromosome spreads, and accelerated development to the morula and blastocyst stage compared with the control and cycloheximide-treated groups, suggesting a role of ploidy in the development of parthenote.
authors.affiliation: Animal Resources Research Center, Kon-Kuk University, Seoul, Korea.
authors.forename: S K
authors.initials: SK
authors.lastname: Cha
authors.forename: N H
authors.initials: NH
authors.lastname: Kim
authors.forename: S M
authors.initials: SM
authors.lastname: Lee
authors.forename: C S
authors.initials: CS
authors.lastname: Baik
authors.forename: H T
authors.initials: HT
authors.lastname: Lee
authors.forename: K S
authors.initials: KS
authors.lastname: Chung
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D002875:Chromosomes / Q000187:drug effects*; D003513:Cycloheximide / Q000494:pharmacology*; D003571:Cytochalasin B / Q000494:pharmacology*; D004558:Electric Stimulation; D005260:Female; D009865:Oocytes / Q000187:drug effects*; D010312:Parthenogenesis / Q000187:drug effects*; D013268:Stimulation, Chemical; D013552:Swine
chemical_list: D003571:Cytochalasin B; D003513:Cycloheximide