Differential in vivo regulation of steroid hormone receptor activation by Cdc37p.
The CDC37 gene is essential for the activity of p60(v-src) when expressed in yeast cells. Since the activation pathway for p60(v-src) and steroid hormone receptors is similar, the present study analyzed the hormone-dependent transactivation by androgen receptors and glucocorticoid receptors in yeast cells expressing a mutant version of the CDC37 gene. In this mutant, hormone-dependent transactivation by androgen receptors was defective at both permissive and restrictive temperatures, although transactivation by glucocorticoid receptors was mildly defective only at the restrictive temperature. Cdc37p appears to function via the androgen receptor ligand-binding domain, although it does not influence receptor hormone-binding affinity. Models for Cdc37p regulation of steroid hormone receptors are discussed.
Fliss
A E
AE
Department of Cell Biology and Anatomy, Mount Sinai Medical Center, New York, New York 10029, USA.
Fang
Y
Y
Boschelli
F
F
Caplan
A J
AJ
D000595:Amino Acid Sequence; D000726:Androgen Antagonists / Q000378:metabolism / Q000494:pharmacology; D018797:Cell Cycle Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D018833:Chaperonins; D003900:Desoxycorticosterone / Q000378:metabolism / Q000494:pharmacology; D013196:Dihydrotestosterone / Q000037:antagonists & inhibitors / Q000378:metabolism / Q000494:pharmacology; D029721:Drosophila Proteins; D005485:Flutamide / Q000031:analogs & derivatives / Q000378:metabolism / Q000494:pharmacology; D017930:Genes, Reporter / Q000235:genetics; D006801:Humans; D008024:Ligands; D018832:Molecular Chaperones; D008969:Molecular Sequence Data; D009154:Mutation / Q000235:genetics; D017434:Protein Structure, Tertiary; D011944:Receptors, Androgen / Q000235:genetics / Q000378:metabolism*; D011965:Receptors, Glucocorticoid / Q000235:genetics / Q000378:metabolism*; D012441:Saccharomyces cerevisiae / Q000187:drug effects / Q000235:genetics*; D029701:Saccharomyces cerevisiae Proteins; D013696:Temperature; D013816:Thermodynamics; D015533:Transcriptional Activation / Q000187:drug effects
D000726:Androgen Antagonists; C513270:CDC37 protein, S cerevisiae; C498362:CDC37 protein, human; D018797:Cell Cycle Proteins; D029721:Drosophila Proteins; D008024:Ligands; D018832:Molecular Chaperones; D011944:Receptors, Androgen; D011965:Receptors, Glucocorticoid; D029701:Saccharomyces cerevisiae Proteins; D013196:Dihydrotestosterone; C014290:hydroxyflutamide; D003900:Desoxycorticosterone; D005485:Flutamide; D018833:Chaperonins