Effects of basic fibroblast growth factor on human periodontal ligament cells.
In order to clarify the regulatory mechanisms of periodontal regeneration by basic fibroblast growth factor (bFGF), effects of bFGF on proliferation, alkaline phosphatase activity, calcified nodule formation and extracellular matrix synthesis of human periodontal ligament (PDL) cells were examined in this study. bFGF enhanced the proliferative responses of PDL cells in a dose-dependent manner. The maximum mitogenic effect of bFGF on PDL cells was observed at the concentration of 10 ng/ml. In contrast, bFGF inhibited the induction of alkaline phosphatase activity and the mineralized nodule formation by PDL cells. Moreover, employing the reverse transcription-polymerase chain reaction (RT-PCR) technique, we observed that the levels of laminin mRNA of human PDL cells was specifically upregulated by bFGF stimulation, but that of type I collagen mRNA was downregulated. On the other hand, the expression of type III collagen and fibronectin mRNA were not altered even when the cells were activated by bFGF. These results suggest that suppressing cytodifferentiation of PDL cells into mineralized tissue forming cells, bFGF may play a role in wound healing by inducing growth of immature PDL cells and that in turn accelerates periodontal regeneration.
Takayama
S
S
Department of Periodontology and Endodontology, Osaka University Faculty of Dentistry, Japan.
Murakami
S
S
Miki
Y
Y
Ikezawa
K
K
Tasaka
S
S
Terashima
A
A
Asano
T
T
Okada
H
H
D000469:Alkaline Phosphatase / Q000187:drug effects / Q000378:metabolism; D002113:Calcification, Physiologic / Q000187:drug effects; D002454:Cell Differentiation / Q000187:drug effects; D002455:Cell Division / Q000187:drug effects; D002478:Cells, Cultured; D003094:Collagen / Q000187:drug effects / Q000235:genetics; D004305:Dose-Response Relationship, Drug; D015536:Down-Regulation / Q000187:drug effects; D005109:Extracellular Matrix / Q000187:drug effects; D016222:Fibroblast Growth Factor 2 / Q000008:administration & dosage / Q000494:pharmacology*; D005353:Fibronectins / Q000187:drug effects / Q000235:genetics; D006801:Humans; D007797:Laminin / Q000187:drug effects / Q000235:genetics; D008934:Mitogens / Q000008:administration & dosage / Q000494:pharmacology; D010513:Periodontal Ligament / Q000166:cytology / Q000187:drug effects* / Q000201:enzymology; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000187:drug effects / Q000235:genetics; D012038:Regeneration; D014158:Transcription, Genetic; D015854:Up-Regulation / Q000187:drug effects; D014945:Wound Healing / Q000187:drug effects
D005353:Fibronectins; D007797:Laminin; D008934:Mitogens; D012333:RNA, Messenger; D016222:Fibroblast Growth Factor 2; D003094:Collagen; D000469:Alkaline Phosphatase