Determination of minute enzymatic activities by means of capillary electrophoretic techniques.
Capillary electrophoretic analysis of enzymes, co-enzymes, substrates and other chemical species that can be linked to an enzymatic reaction is reviewed with 80 references. Both off-line and on-line assays of minute enzymatic activities are discussed. In addition to heterogeneous on-line enzyme assays, a special emphasis is given to a newly established on-line technique called electrophoretically mediated microanalysis (EMMA). The basic principle, procedure, and various detection modes of EMMA are discussed. The recent developments in on-line determination of various enzyme substrates as well as on-line enzyme kinetic studies are also summarized. Some potential future developments in the determination of enzymatic activities by means of CE are also presented.
Bao
J J
JJ
Procter & Gamble Pharmaceuticals, Cincinnati, OH 45241, USA.
Fujima
J M
JM
Danielson
N D
ND
D019075:Electrophoresis, Capillary / Q000379:methods*; D004798:Enzymes / Q000032:analysis* / Q000378:metabolism; D007700:Kinetics; D009862:Online Systems
D004798:Enzymes