Nuclear translocation of the Y-box binding protein by ultraviolet irradiation.
The Y-box binding protein, YB-1, is a member of a DNA binding protein family with a structurally and functionally conserved cold shock domain. Using Western blotting and immunohistochemical methods, larger amounts of YB-1 were detected in the cytosol, particularly at the perinuclear region, than in the nucleus of human cancer cells. UV irradiation increased accumulation of YB-1 in the nucleus at 20 min and thereafter. This translocation of YB-1 into the nucleus by UV irradiation was blocked by the protein kinase inhibitor H-7, but not HA-1004. Both green fluorescent protein (GFP)-YB-1 and GFP-YB-1C with the C-terminus (248-317) of YB-1 were located mainly in the cytosol, but GFP-YB-1deltaC with a deletion at the C-terminus of YB-1 was located in the nucleus. YB-1 is translocated into the nucleus by UV irradiation, possibly through a protein kinase C-mediated signal transduction pathway, and the C-terminal region of YB-1 might be important for cytoplasmic retention of YB-1.
Koike
K
K
Department of Biochemistry, Kyushu University School of Medicine, Fukuoka, Japan. xtckk@biochem1.med.kyushu-u.ac.jp
Uchiumi
T
T
Ohga
T
T
Toh
S
S
Wada
M
M
Kohno
K
K
Kuwano
M
M
D019307:1-(5-Isoquinolinesulfonyl)-2-Methylpiperazine / Q000494:pharmacology; D000818:Animals; D022762:CCAAT-Enhancer-Binding Proteins; D019556:COS Cells; D002467:Cell Nucleus / Q000378:metabolism* / Q000528:radiation effects; D004268:DNA-Binding Proteins / Q000378:metabolism* / Q000528:radiation effects; D004791:Enzyme Inhibitors / Q000494:pharmacology; D019084:Fluorescent Antibody Technique, Indirect; D049452:Green Fluorescent Proteins; D006801:Humans; D007546:Isoquinolines / Q000494:pharmacology; D007624:KB Cells / Q000528:radiation effects*; D008164:Luminescent Proteins / Q000378:metabolism; D051839:NFI Transcription Factors; D009687:Nuclear Proteins; D047428:Protein Kinase Inhibitors; D011993:Recombinant Fusion Proteins / Q000378:metabolism; D013449:Sulfonamides; D014157:Transcription Factors / Q000378:metabolism; D014162:Transfection; D014407:Tumor Cells, Cultured; D014466:Ultraviolet Rays; D001749:Urinary Bladder Neoplasms; D051840:Y-Box-Binding Protein 1
D022762:CCAAT-Enhancer-Binding Proteins; D004268:DNA-Binding Proteins; D004791:Enzyme Inhibitors; D007546:Isoquinolines; D008164:Luminescent Proteins; D051839:NFI Transcription Factors; D009687:Nuclear Proteins; D047428:Protein Kinase Inhibitors; D011993:Recombinant Fusion Proteins; D013449:Sulfonamides; D014157:Transcription Factors; D051840:Y-Box-Binding Protein 1; C070856:YBX1 protein, human; D049452:Green Fluorescent Proteins; D019307:1-(5-Isoquinolinesulfonyl)-2-Methylpiperazine; C043204:N-(2-guanidinoethyl)-5-isoquinolinesulfonamide