Quantification of conserved antigens in Helicobacter pylori during different culture conditions.
In this study, we raised monoclonal antibodies (MAbs) against three conserved Helicobacter pylori antigens, i.e., the N-acetylneuraminyllactose-binding fibrillar hemagglutinin, HpaA; the flagellin subunits, FlaA and FlaB; and a species-specific 26-kDa protein. The MAbs were used for the development of sensitive inhibition enzyme-linked immunosorbent assays for quantification of these antigens in H. pylori during various culture conditions. The quantities of these antigens varied considerably (up to 8-fold) during different culture procedures and between strains (up to 10-fold).
Lindholm
C
C
Department of Medical Microbiology and Immunology, Göteborg University, Sweden.
Osek
J
J
Svennerholm
A M
AM
D000907:Antibodies, Bacterial / Q000276:immunology; D000911:Antibodies, Monoclonal / Q000276:immunology; D000942:Antigens, Bacterial / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay; D016480:Helicobacter pylori / Q000254:growth & development / Q000276:immunology*
D000907:Antibodies, Bacterial; D000911:Antibodies, Monoclonal; D000942:Antigens, Bacterial