The pituitary folliculo-stellate cell line TtT/GF augments basal and TRH-induced prolactin secretion by GH3 cell.
Numerous functions have been ascribed to anterior pituitary folliculo-stellate (FS) cells. Recently, we established a pituitary FS-like cell line (TtT/GF) from an isologously transplantable pituitary thyrotropic tumor. Here we investigated the effect of FS cells on prolactin (PRL) secretion by using a co-culture system between TtT/GF cells and GH3 cells. We observed that co-culture with TtT/GF cells significantly increased basal PRL secretion without affecting the growth rate of GH3 cells. Further, addition of anti-cytokine-induced neutrophil chemoattractant (CINC) antibody partially reduced PRL secretion in co-culture of GH3 cells with 10% TtT/GF cells. Co-culture with 5% TtT/GF cells also markedly enhanced the PRL response to thyrotropin-releasing hormone (TRH) by GH3 cells, while addition of anti-CINC antibody had no effects on the enhanced PRL response to TRH. These results suggest that FS cells augment basal PRL secretion and enhance the secretory response of PRL to TRH by a paracrine mechanism.
Koike
K
K
Department of Obstetrics and Gynecology, School of Medicine, Kanazawa University, Ishikawa, Japan.
Zhang
Z X
ZX
Sakamoto
Y
Y
Kanda
Y
Y
Murakami
K
K
Miyake
A
A
Inoue
M
M
D000818:Animals; D000906:Antibodies / Q000276:immunology; D002455:Cell Division / Q000502:physiology; D002460:Cell Line; D002630:Chemotactic Factors / Q000276:immunology; D018920:Coculture Techniques; D051379:Mice; D010903:Pituitary Gland, Anterior / Q000166:cytology / Q000378:metabolism / Q000502:physiology*; D011388:Prolactin / Q000378:metabolism*; D013973:Thyrotropin-Releasing Hormone / Q000502:physiology*
D000906:Antibodies; D002630:Chemotactic Factors; D013973:Thyrotropin-Releasing Hormone; D011388:Prolactin