G-protein-coupled receptors in Saccharomyces cerevisiae: high-throughput screening assays for drug discovery.
G-protein-coupled receptors are an important class of therapeutic drug targets by virtue of their roles in the regulation of diverse cellular functions. Recent advances in the expression of heterologous G-protein-coupled receptors in the yeast Saccharomyces cerevisiae have led to the development of sensitive and selective assays of their ligand-induced activation. Implementation of this new technology in the high-throughput screening of compound libraries has enabled the discovery of novel ligands for the G-protein-coupled somatostatin receptor. This article describes the broad applicability of the technology and its use in drug discovery.
Pausch
M H
MH
Cyanamid Agricultural Research Center, American Cyanamid Company, Princeton, NJ 08543-0400, USA. pauschm@pt.cyanamid.com
D004345:Drug Industry / Q000639:trends*; D019204:GTP-Binding Proteins; D017481:Receptors, Somatostatin; D012441:Saccharomyces cerevisiae / Q000737:chemistry*
D017481:Receptors, Somatostatin; D019204:GTP-Binding Proteins