A role for P-selectin in neutrophil and platelet infiltration in immune complex glomerulonephritis.
P-selectin is one of the key early mediators of leukocyte adhesion in inflammatory conditions. This report examines the role of P-selectin in a neutrophil- and platelet-mediated model of glomerulonephritis (the concanavalin A [con A] model). The administration of neutralizing anti-P-selectin antibody (PB 1.3) reduced the platelet influx at 10 min (P < 0.05) and was associated with a 60% reduction in the neutrophil infiltrate and a 50% reduction in the number of oxidant-producing cells at 3 h within glomeruli. No effect on glomerular monocyte-macrophage accumulation was observed, and proteinuria was reduced by 20% but did not reach significance. It is concluded that P-selectin plays an important role in mediating the neutrophil and platelet accumulation in this model and likely has a role in mediating the glomerular injury.
Zachem
C R
CR
Department of Medicine, University of Washington, Seattle, USA.
Alpers
C E
CE
Way
W
W
Shankland
S J
SJ
Couser
W G
WG
Johnson
R J
RJ
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology / Q000494:pharmacology / Q000627:therapeutic use; D001792:Blood Platelets / Q000473:pathology*; D002448:Cell Adhesion; D002634:Chemotaxis, Leukocyte; D003176:Complement C3 / Q000032:analysis; D003208:Concanavalin A / Q000008:administration & dosage / Q000276:immunology / Q000633:toxicity; D004730:Endothelium, Vascular / Q000293:injuries / Q000473:pathology; D005921:Glomerulonephritis / Q000139:chemically induced / Q000473:pathology* / Q000517:prevention & control; D007105:Immune Complex Diseases / Q000139:chemically induced / Q000473:pathology* / Q000517:prevention & control; D007074:Immunoglobulin G / Q000276:immunology / Q000633:toxicity; D007261:Infusions, Intra-Arterial; D007678:Kidney Glomerulus / Q000473:pathology; D008297:Male; D009504:Neutrophils / Q000473:pathology*; D019007:P-Selectin / Q000276:immunology / Q000502:physiology*; D010973:Platelet Adhesiveness; D051381:Rats; D017207:Rats, Sprague-Dawley; D017382:Reactive Oxygen Species; D012077:Renal Artery
D000911:Antibodies, Monoclonal; D003176:Complement C3; D007074:Immunoglobulin G; D019007:P-Selectin; D017382:Reactive Oxygen Species; D003208:Concanavalin A