Cloning of P2Y6 cDNAs and identification of a pseudogene: comparison of P2Y receptor subtype expression in bone and brain tissues.
Cellular responses to ATP/UTP and analogs are mediated by G-protein coupled P2Y receptors and have been proposed to play a role in the regulation of bone metabolism. Using a degenerate PCR approach on MG-63 cell cDNA we found PCR fragments coding for human P2Y1 and a new receptor, P2Y6. cDNA cloning of the P2Y6 receptor identified three of cDNA isoforms. Two contained the same contiguous ORFs but differed in their 5 UTRs and may therefore originate by alternative splicing whereas the third represents a pseudogene. Analysis of P2Y receptor subtype expression in human bone and the osteoblastic cell lines OHS-4 and MG-63 by RT-PCR showed that all known human P2Y receptor subtypes (P2Y1, P2Y2, P2Y4, P2Y6, and P2Y7) were expressed. In contrast, analysis of brain-derived cell lines suggests that a selective expression of P2Y receptor subtypes occurs in brain tissue.
Maier
R
R
Novartis Pharma Inc., Basel, Switzerland.
Glatz
A
A
Mosbacher
J
J
Bilbe
G
G
D017398:Alternative Splicing; D015152:Blotting, Northern; D001842:Bone and Bones / Q000378:metabolism*; D001921:Brain / Q000378:metabolism*; D002460:Cell Line; D003001:Cloning, Molecular; D018076:DNA, Complementary; D015870:Gene Expression; D006801:Humans; D016366:Open Reading Frames; D010006:Osteoblasts; D016133:Polymerase Chain Reaction / Q000379:methods; D011544:Pseudogenes; D018048:Receptors, Purinergic P2 / Q000096:biosynthesis / Q000145:classification / Q000235:genetics*
D018076:DNA, Complementary; D018048:Receptors, Purinergic P2; C099845:purinoceptor P2Y6