Lipid mediators modulate NMDA receptor currents in a Xenopus oocyte expression system.
We determined the modulatory effects of various lipid mediators on mouse N-methyl-D-aspartate (NMDA) receptor currents in the Xenopus oocyte expression system. Arachidonic acid, but not oleic acid potentiated NMDA receptor activity. The epsilon1/zeta1 heterodimer of the NMDA receptor was more sensitive to arachidonic acid than was the epsilon2/zeta1 heterodimer. Platelet-activating factor (PAF) and lysophosphatidic acid (LPA) both activated the NMDA currents, and the effects were more evident in the epsilon2/zeta1 heterodimer than in epsilon1/zeta1. These activations were abolished by treatment with protein kinase inhibitors, suggesting a possible phosphorylation of the receptor. Thus, lipid mediators do have modulatory effects on NMDA receptor currents, the potentiating effects of which differ depending on subtype of the NMDA receptor.
Tabuchi
S
S
Department of Biochemistry and Molecular Biology, Faculty of Medicine, The University of Tokyo, Japan.
Kume
K
K
Aihara
M
M
Ishii
S
S
Mishina
M
M
Shimizu
T
T
D000818:Animals; D016718:Arachidonic Acid / Q000494:pharmacology; D008055:Lipids / Q000494:pharmacology*; D008246:Lysophospholipids / Q000494:pharmacology; D051379:Mice; D019301:Oleic Acid / Q000494:pharmacology; D009865:Oocytes; D010766:Phosphorylation; D010972:Platelet Activating Factor / Q000494:pharmacology; D047428:Protein Kinase Inhibitors; D012333:RNA, Messenger; D016194:Receptors, N-Methyl-D-Aspartate / Q000187:drug effects / Q000502:physiology*; D014982:Xenopus laevis
D008055:Lipids; D008246:Lysophospholipids; D010972:Platelet Activating Factor; D047428:Protein Kinase Inhibitors; D012333:RNA, Messenger; D016194:Receptors, N-Methyl-D-Aspartate; D016718:Arachidonic Acid; D019301:Oleic Acid