title: Use of intratracheal pulmonary ventilation versus conventional ventilation in meconium aspiration syndrome in a newborn pig model.
abstract: OBJECTIVE
  To determine whether intratracheal pulmonary ventilation (ITPV) allows for effective oxygenation and ventilation at lower mean airway pressures and peak inspiratory pressures than conventional ventilation in a piglet model of meconium aspiration syndrome.
  
  
  METHODS
  Prospective, interventional study.
  
  
  METHODS
  The animal research laboratory at Children's National Medical Center, Washington, DC.
  
  
  METHODS
  Twenty newborn piglets, 2 to 7 days of age, weighing 1.8 to 2.8 kg.
  
  
  METHODS
  Animals were anesthetized, paralyzed, intubated, and ventilated. Femoral arterial and venous catheters were inserted; 5 mL/kg of 20% meconium in normal saline was instilled into the endotracheal tube. Animals were randomized to either ITPV or conventional ventilation, and settings were adjusted to maintain ideal blood gases, i.e., pH 7.35 to 7.45, PCO2 40 to 45 torr (5.3 to 6 kPa), PO2 80 to 100 torr (10.7 to 13.3 kPa), and SaO2 > or = 90%. Ventilatory settings were adjusted as needed to a maximum of: FIO2 1.0, peak inspiratory pressure 40 cm H2O, positive end-expiratory pressure 5 cm H2O, and respiratory rate 80 breaths/min.
  
  
  RESULTS
  Arterial blood gases were taken every 30 mins for 4 hrs and ventilatory settings were adjusted to maintain optimal blood gases. Heart rate, mean arterial blood pressure, and arterial saturation were monitored continuously. The animals in the ITPV group had significantly lower peak inspiratory pressure at 1, 2, 3, and 4 hrs after meconium instillation (p < .018) and significantly lower mean airway pressure at 2, 3, and 4 hrs after meconium instillation (p < .03). The mean peak inspiratory pressure in the ITPV animals ranged from 17 +/- 2.7 cm H2O at baseline to 16.6 +/- 5.7 cm H2O at 4 hrs compared with 16.5 +/- 2.7 cm H2O at baseline to 31.8 +/- 9.1 cm H2O at 4 hrs in the conventionally ventilated animals (p < .04). The mean airway pressure ranged from 6.3 +/- 1.1 mm Hg at baseline to 6.8 +/- 2.5 mm Hg at 4 hrs in the ITPV group compared with 5.5 +/- 1.2 mm Hg at baseline to 10.7 +/- 3.4 mm Hg at 4 hrs in the conventional ventilation group (p < .03). The lungs of the ITPV animals were less hemorrhagic and had less pathologic evidence of injury than the lungs of the conventionally ventilated animals.
  
  
  CONCLUSIONS
  These results indicate that ITPV can be used to effectively ventilate and oxygenate piglets with meconium aspiration syndrome at lower mean airway pressures and peak inspiratory pressures than conventional ventilation. This lower pressure causes less injury to the lungs of the animals.
authors.affiliation: George Washington University School of Medicine, Washington, DC, USA.
authors.forename: H
authors.initials: H
authors.lastname: Handman
authors.forename: K
authors.initials: K
authors.lastname: Rais-Bahrami
authors.forename: O
authors.initials: O
authors.lastname: Rivera
authors.forename: W R
authors.initials: WR
authors.lastname: Seale
authors.forename: B L
authors.initials: BL
authors.lastname: Short
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D004195:Disease Models, Animal; D006612:High-Frequency Ventilation / Q000009:adverse effects; D006801:Humans; D007231:Infant, Newborn; D007442:Intubation, Intratracheal; D008168:Lung / Q000473:pathology / Q000503:physiopathology; D008471:Meconium Aspiration Syndrome / Q000628:therapy*; D012121:Respiration, Artificial / Q000295:instrumentation / Q000379:methods*; D013552:Swine