Determination of omeprazole in pharmaceuticals by derivative spectroscopy.
A new derivative UV spectroscopic method was developed for the analysis of omeprazole in borate buffer (pH 10.0; 0.1 M). Second derivative spectra were generated between 200-400 nm at N = 9, delta gamma = 31.5. The linearity range for values obtained from second derivative spectra was 0.2-40.0 micrograms ml-1. The developed method was applied to five different commercial preparations of hard gelatin capsules containing enteric coated granules. The relative standard deviations were found to be 2.24% (brand A), 1.87% (brand B), 2.80% (brand C), 4.55% (brand D) and 1.09% (brand E). The data were compared with ones obtained from the polarographic method given in the literature and no difference was found statistically.
Ozaltin
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Department of Analytical Chemistry, Faculty of Pharmacy, Hacettepe University, Ankara, Turkey.
Koçer
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A
D000897:Anti-Ulcer Agents / Q000032:analysis* / Q000737:chemistry; D002214:Capsules; D009853:Omeprazole / Q000032:analysis* / Q000737:chemistry; D004364:Pharmaceutical Preparations / Q000032:analysis; D013056:Spectrophotometry, Ultraviolet / Q000379:methods*; D013057:Spectrum Analysis
D000897:Anti-Ulcer Agents; D002214:Capsules; D004364:Pharmaceutical Preparations; D009853:Omeprazole