Capillary electrophoresis of DNA restriction fragments: effect of polymer properties.
The mechanism of DNA separation by dilute polymer solutions in capillary electrophoresis is not well understood. To provide information on the effect of polymer properties on DNA separations, four polymers that differ in size, shape and stiffness were examined. Hydroxyethylcellulose of high molecular weight provides excellent separation of large DNA fragments (2027 bp - 23,130 bp). Polyvinylpyrrolidone separates DNA from 72 bp to 23 kbp; star-poly(ethylene oxide) and linear poly(ethylene oxide) provide separation of fragments to 1353 bp.
Braun
B
B
Department of Chemical Engineering, University of California at Berkeley, 94720, USA.
Blanch
H W
HW
Prausnitz
J M
JM
D001482:Base Composition; D002482:Cellulose / Q000031:analogs & derivatives / Q000737:chemistry; D055598:Chemical Phenomena; D002627:Chemistry, Physical; D004247:DNA / Q000302:isolation & purification*; D004262:DNA Restriction Enzymes; D019075:Electrophoresis, Capillary / Q000379:methods*; D008970:Molecular Weight; D011092:Polyethylene Glycols / Q000737:chemistry; D011108:Polymers; D011205:Povidone / Q000737:chemistry; D012996:Solutions; D014783:Viscosity
D011108:Polymers; D012996:Solutions; D011092:Polyethylene Glycols; D002482:Cellulose; C002283:hydroxyethylcellulose; D004247:DNA; D004262:DNA Restriction Enzymes; D011205:Povidone