Topical application of 5-HT antibodies reduces edema and cell changes following trauma of the rat spinal cord.
Involvement of serotonin in the early microvascular reactions and cell changes following trauma of the spinal cord was examined using topical application of serotonin antibodies to the traumatised cord in a rat model. A focal trauma of the spinal cord was produced by making an incision into the right dorsal horn of the T10-11 segments (about 2 mm deep and 5 mm long); the animals were allowed to survive 5 h after injury. Monoclonal 5-HT antibodies (1:20) were applied (25 microliters in 10 sec) to the traumatised spinal cord 2 min after injury. There was a significant reduction in the breakdown of the blood-spinal cord barrier, edema formation, and cell changes in the traumatised rats which received 5-HT antiserum compared to the injured rats given saline. These results show that 5-HT is an important mediator involved in the early pathophysiological responses of spinal cord injury.
Sharma
H S
HS
Department of Anatomy, Uppsala University, Sweden.
Westman
J
J
Nyberg
F
F
D000287:Administration, Topical; D000818:Animals; D000911:Antibodies, Monoclonal / Q000627:therapeutic use*; D002199:Capillary Permeability; D004487:Edema / Q000628:therapy; D008297:Male; D051381:Rats; D017208:Rats, Wistar; D012701:Serotonin / Q000276:immunology*; D013116:Spinal Cord / Q000098:blood supply / Q000473:pathology; D013119:Spinal Cord Injuries / Q000473:pathology / Q000628:therapy*
D000911:Antibodies, Monoclonal; D012701:Serotonin