Transport of paraquat by a renal epithelial cell line, MDCK.
Transport of paraquat (PQ), a herbicidal cation, was previously investigated in a proximal (LLC-PK1), renal epithelial cell line using permeable collagen-coated filters. PQ was actively transported from the basolateral side via a cation transport system by the LLC-PK1 cells. In the present study, the transport of PQ was investigated in a distal renal epithelial cell line, MDCK. PQ was predominantly transported from the basolateral to apical (B to A) side. The basolateral transport of PQ in MDCK cells was not saturable with increasing concentrations and not energy dependent. The flux and uptake of PQ was much lower in the MDCK than LLC-PK1 cells. It is concluded that MDCK, a distal renal tubular cell line, does not have an active transport system for PQ.
Chan
B S
BS
Department of Renal Medicine, Royal Prince Alfred Hospital, Camperdown, Australia. Bchan@renicu.rpa.cs.nsw.gov.au
Lazzaro
V A
VA
Seale
J P
JP
Duggin
G G
GG
D000818:Animals; D001693:Biological Transport, Active / Q000187:drug effects; D002460:Cell Line; D002478:Cells, Cultured; D004140:Dinitrophenols / Q000494:pharmacology; D004285:Dogs; D004848:Epithelium / Q000378:metabolism; D006540:Herbicides / Q000493:pharmacokinetics*; D007686:Kidney Tubules, Distal / Q000378:metabolism*; D007687:Kidney Tubules, Proximal / Q000378:metabolism; D010085:Oxidative Phosphorylation / Q000187:drug effects; D010269:Paraquat / Q000493:pharmacokinetics*
D004140:Dinitrophenols; D006540:Herbicides; D010269:Paraquat