Increased apoptosis of adult rat lymphocytes after single neonatal vitamin A treatment (hormonal imprinting). A flow cytometric analysis.
Newborn rats were treated with a single dose of vitamin A (retinol) and apoptosis of peripheral lymphocytes was studied by flow cytometry in adult age. Vitamin A treatment (hormonal imprinting) caused a moderate, however significant elevation in the number of apoptotic lymphocytes after three months. Dexamethasone or Concanavalin-A alone did not influence apoptosis significantly. However, in the neonatally retinol treated rats dexamathasone significantly elevated the quantity of apoptotic lymphocytes related to the control or Concanavalin-A treated control cells. The results call attention to the prolonged effect of hormonal imprinting in a new index and to the possible dangerous effects in human, neonatally treated with vitamin A.
Gaál
A
A
Department of Genetics, Cell and Immunobiology, Semmelweis University of Medicine, Budapest, Hungary.
Bocsi
J
J
Falus
A
A
Szende
B
B
Csaba
G
G
D000818:Animals; D000831:Animals, Newborn; D017209:Apoptosis / Q000187:drug effects*; D005260:Female; D005434:Flow Cytometry; D008214:Lymphocytes / Q000187:drug effects*; D051381:Rats; D017208:Rats, Wistar; D014801:Vitamin A / Q000494:pharmacology*
D014801:Vitamin A