High resolution multichannel fluorescence detection for capillary electrophoresis. Application to multicomponent analysis.
A wavelength-resolved fluorescence detector for laser-induced fluorescence detection in capillary electrophoresis (CE) is described that uses a charge injection device (CID) array detector Post-column fluorescence detection occurs using a sheath flow cell. The limit of detection for fluorescein is 4.8 x 10(-11) M (29,000 molecules), the spectral resolution is 0.56 nm/pixel, and the spectrograph/CID monitors a 250 nm spectrum throughout the 250-875 nm range. Custom array readout, data manipulation and data processing methods are described to convert wavelength/spatial CID images into electropherograms. The application of the system to characterizing bilirubins in human serum is described, demonstrating the ability to match electrophoretic peaks to standards using spectral information.
Oldenburg
K E
KE
Department of Chemistry, Beckman Institute, University of Illinois at Urbana-Champaign 61801, USA.
Xi
X
X
Sweedler
J V
JV
D001663:Bilirubin / Q000097:blood; D019075:Electrophoresis, Capillary / Q000295:instrumentation*; D016650:Fluorescein-5-isothiocyanate; D006801:Humans; D007202:Indicators and Reagents; D013050:Spectrometry, Fluorescence / Q000295:instrumentation*
D007202:Indicators and Reagents; D016650:Fluorescein-5-isothiocyanate; D001663:Bilirubin