title: Quantification of cytokine gene expression in lamina propria lymphocytes of cattle following infection with Ostertagia ostertagi.
abstract: Changes in cell surface markers and cytokine transcription were analyzed in lamina propria lymphocytes from control animals (noninfected calves) and calves after a single high but nonprotective primary infection with Ostertagia ostertagi. Flow cytometry of cells recovered from the lamina propria showed an increase in the percentages of IgM+, WC1+, and IL-2R+-bearing cells 10 days after infection; however, 2 mo after infection, cell staining was comparable to preinfection levels. Transcription levels of interleukins IL-2, IL-4, IL-10, and interferon (IFN)-gamma mRNA were measured using a competitive reverse transcription-polymerase chain reaction. Results indicated elevated levels of IL-4 and IFN-gamma in the infected animals at 10 days and at 60 days after infection. Transcription of IL-10 also increased; however, this change was not observed until 60 days postinfection.
authors.affiliation: Immunology and Disease Resistance Laboratory, LPSI, ARS, USDA, Beltsville, Maryland 20705, USA.
authors.forename: S
authors.initials: S
authors.lastname: Almeria
authors.forename: A
authors.initials: A
authors.lastname: Canals
authors.forename: D S
authors.initials: DS
authors.lastname: Zarlenga
authors.forename: L C
authors.initials: LC
authors.lastname: Gasbarre
mesh_terms: D000818:Animals; D000954:Antigens, Surface / Q000032:analysis; D015415:Biomarkers / Q000032:analysis; D002417:Cattle; D016207:Cytokines / Q000235:genetics*; D005786:Gene Expression Regulation / Q000276:immunology*; D007413:Intestinal Mucosa / Q000276:immunology / Q000378:metabolism* / Q000469:parasitology*; D016131:Lymphocyte Subsets / Q000145:classification / Q000378:metabolism*; D010028:Ostertagia / Q000276:immunology*; D010029:Ostertagiasis / Q000235:genetics / Q000276:immunology*; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000032:analysis; D014158:Transcription, Genetic
chemical_list: D000954:Antigens, Surface; D015415:Biomarkers; D016207:Cytokines; D012333:RNA, Messenger