title: Immunolocalization of vacuolar-type H+-ATPase in rat submandibular gland and adaptive changes induced by acid-base disturbances.
abstract: Using antibodies against the 31-kD and 70-kD subunits of vacuolar type H+-ATPase (V-ATPase) and light microscopic immunocytochemistry, we have demonstrated the presence of this V-ATPase in rat submandibular gland. We have also investigated the adaptive changes of this transporter during acid-base disturbances such as acute and chronic metabolic acidosis or alkalosis. Our results show intracellularly distributed V-ATPase in striated, granular, and main excretory duct cells in controls, but no V-ATPase immunoreaction in acinar cells. Both acute and chronic metabolic acidosis caused a shift in V-ATPase away from diffuse distribution towards apical localization in striated and granular duct cells, suggesting that a V-ATPase could be involved in the regulation of acid-base homeostasis. In contrast, during acidosis the main excretory duct cells showed no changes in the V-ATPase distribution compared to controls. With acute and chronic metabolic alkalosis, no changes in the V-ATPase distribution occurred. (J Histochem Cytochem 46:91-100, 1998)
authors.affiliation: Anatomisches Institut, Medizinische Fakultät, Universität des Saarlandes, Homburg/Saar, Germany.
authors.forename: E
authors.initials: E
authors.lastname: Roussa
authors.forename: F
authors.initials: F
authors.lastname: Thévenod
authors.forename: I
authors.initials: I
authors.lastname: Sabolic
authors.forename: C M
authors.initials: CM
authors.lastname: Herak-Kramberger
authors.forename: W
authors.initials: W
authors.lastname: Nastainczyk
authors.forename: R
authors.initials: R
authors.lastname: Bock
authors.forename: I
authors.initials: I
authors.lastname: Schulz
mesh_terms: D000137:Acid-Base Imbalance / Q000139:chemically induced; D000138:Acidosis / Q000139:chemically induced / Q000201:enzymology; D000222:Adaptation, Physiological / Q000187:drug effects; D000471:Alkalosis / Q000139:chemically induced / Q000201:enzymology; D000643:Ammonium Chloride / Q000494:pharmacology; D000818:Animals; D015153:Blotting, Western; D007150:Immunohistochemistry; D008297:Male; D006180:Proton-Translocating ATPases / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar; D018987:Salivary Ducts / Q000166:cytology / Q000187:drug effects / Q000201:enzymology; D017693:Sodium Bicarbonate / Q000494:pharmacology; D013363:Submandibular Gland / Q000166:cytology / Q000187:drug effects / Q000201:enzymology*; D025262:Vacuolar Proton-Translocating ATPases
chemical_list: D000643:Ammonium Chloride; D017693:Sodium Bicarbonate; D025262:Vacuolar Proton-Translocating ATPases; D006180:Proton-Translocating ATPases