A simple and objective approach to identifying human round spermatids.
Although round spermatids have been studied extensively using staining techniques and electron microscopy, little information is available about their appearance in living conditions. We describe a method of collecting and identifying round spermatids from ejaculates and testicular biopsies. The validity of the selection procedure was confirmed by fluorescence in-situ hybridization. Based on cell size, morphological characteristics of nucleus and cytoplasm, and on the nucleus/cytoplasm ratio, we harvested a population of cells that was 84% haploid. This procedure can be applied to select spermatids for clinical or research purposes.
Angelopoulos
T
T
Department of Obstetrics and Gynecology, New York University Medical Center, NY 10016, USA.
Krey
L
L
McCullough
A
A
Adler
A
A
Grifo
J A
JA
D001706:Biopsy; D002467:Cell Nucleus / Q000648:ultrastructure; D002469:Cell Separation / Q000379:methods; D002499:Centrifugation, Density Gradient; D003593:Cytoplasm / Q000648:ultrastructure; D006238:Haploidy; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D008297:Male; D008853:Microscopy / Q000379:methods; D013087:Spermatids / Q000648:ultrastructure*; D013194:Staining and Labeling; D013737:Testis / Q000166:cytology