title: Comparison of in vivo cardiac function with ex vivo cardiac performance of the rat heart after thoracic irradiation.
abstract: The aim of the study was to compare in vivo cardiac function with ex vivo cardiac performance after local heart irradiation in the same rat. Left ventricular ejection fraction (LVEF) was measured in vivo by radionuclide ventriculography in Sprague-Dawley rats up to 16 months after a single dose of 20 Gy. Four days after in vivo measurements, cardiac performance was determined ex vivo, using the isolated working rat heart preparation. After irradiation, cardiac performance measured ex vivo deteriorated more rapidly than the in vivo measured LVEF. Within 4 months post-treatment, ex vivo cardiac output and stroke volume started to decrease and declined continuously throughout the observation period of 16 months. The reduction in stroke volume was already significant (p < 0.04) at 4 months post-treatment, whereas the decline in cardiac output was significant (p < 0.05) at 12 months post-treatment. In vivo, no change in LVEF was observed during the first 12 months post-treatment. Thereafter, LVEF decreased rapidly from 65 +/- 2% to 46 +/- 8% (p < 0.01), at 16 months post-treatment. Up to 12 months post-irradiation, LVEF was not correlated to ex vivo cardiac output. At 16 months post-treatment, when clinical symptoms of heart failure become evident, a positive relation between both parameters was found. The lack of correlation between the in vivo and ex vivo measurements of cardiac function during the first 12 months post-treatment might be explained by the involvement of compensatory mechanisms being operative in vivo to maintain sufficient cardiac output.
authors.affiliation: Department of Clinical Oncology, University Hospital Leiden, The Netherlands.
authors.forename: N A
authors.initials: NA
authors.lastname: Franken
authors.forename: J A
authors.initials: JA
authors.lastname: Camps
authors.forename: F J
authors.initials: FJ
authors.lastname: van Ravels
authors.forename: A
authors.initials: A
authors.lastname: van der Laarse
authors.forename: E K
authors.initials: EK
authors.lastname: Pauwels
authors.forename: J
authors.initials: J
authors.lastname: Wondergem
mesh_terms: D000818:Animals; D002302:Cardiac Output / Q000528:radiation effects*; D005260:Female; D006321:Heart / Q000503:physiopathology / Q000528:radiation effects*; D006333:Heart Failure / Q000209:etiology / Q000503:physiopathology; D011833:Radiation Injuries, Experimental / Q000503:physiopathology; D015635:Radionuclide Ventriculography; D051381:Rats; D017207:Rats, Sprague-Dawley; D013318:Stroke Volume / Q000528:radiation effects; D013997:Time Factors