Induction of thioredoxin in human lymphocytes with low-dose ionizing radiation.
Induction of the expression of the thioredoxin (TRX) gene, producing a key protein in regulating cellular functions through redox reaction as well as being a radioprotector, was followed after ionizing irradiation of lymphocytes from human donors. The TRX mRNA level increased to a peak, 5.7-fold higher than the control at maximum, 6 h after irradiation, and then decreased. The optimum radiation dose for enhancement of induction of the TRX mRNA was 0.25 Gy. The TRX protein also increased to a peak, a 3-fold increase at maximum, with the same timing as that for TRX mRNA.
Hoshi
Y
Y
Nuclear Energy Systems Department, Central Research Institute of Electric Power Industry, Komae-shi, Tokyo, Japan. hoshi@criepi.denken.or.jp
Tanooka
H
H
Miyazaki
K
K
Wakasugi
H
H
D015152:Blotting, Northern; D005786:Gene Expression Regulation / Q000528:radiation effects*; D006801:Humans; D007700:Kinetics; D008214:Lymphocytes / Q000378:metabolism / Q000528:radiation effects*; D012333:RNA, Messenger / Q000032:analysis / Q000235:genetics; D011839:Radiation, Ionizing; D013879:Thioredoxins / Q000096:biosynthesis* / Q000235:genetics
D012333:RNA, Messenger; D013879:Thioredoxins