Expression of the mouse histone gene H1t begins at premeiotic stages of spermatogenesis.
The gene encoding H1t, a testicular variant of histone H1, is expressed in mammals during spermatogenesis. Northern blot and in situ hybridization has detected H1t mRNA only at the stage of pachytene spermatocytes. We have extended this analysis to more sensitive approaches and demonstrate, by RNase protection and electron-microscopic in situ hybridization, that H1t mRNA is detectable even in spermatogonia. Just a faint H1t band is seen in Western blots of nuclear protein from 9-day-old mice. This indicates that the H1t gene is expressed at premeiotic stages, albeit at a low level. In contrast to H1t mRNA, the H1t protein has not been detected in spermatogonia by electron microscopy after immunogold staining.
Drabent
B
B
Division of Molecular Biology, Department of Biochemistry, Georg-August Universität Göttingen, Humboldtallee 23, D-37073 Göttingen, Germany.
Bode
C
C
Miosge
N
N
Herken
R
R
Doenecke
D
D
D000818:Animals; D000918:Antibody Specificity; D015153:Blotting, Western; D015870:Gene Expression / Q000502:physiology; D006657:Histones / Q000032:analysis / Q000235:genetics* / Q000276:immunology; D007150:Immunohistochemistry; D017403:In Situ Hybridization; D008297:Male; D008540:Meiosis / Q000502:physiology*; D051379:Mice; D008815:Mice, Inbred Strains; D016253:Microscopy, Immunoelectron; D012333:RNA, Messenger / Q000032:analysis; D013087:Spermatids / Q000737:chemistry / Q000648:ultrastructure; D013091:Spermatogenesis / Q000502:physiology*; D013093:Spermatogonia / Q000737:chemistry / Q000648:ultrastructure; D013737:Testis / Q000737:chemistry / Q000166:cytology / Q000254:growth & development
D006657:Histones; D012333:RNA, Messenger