Identification of clonogenic common lymphoid progenitors in mouse bone marrow.
The existence of a common lymphoid progenitor that can only give rise to T cells, B cells, and natural killer (NK) cells remains controversial and constitutes an important gap in the hematopoietic lineage maps. Here, we report that the Lin(-)IL-7R(+)Thy-1(-)Sca-1loc-Kit(lo) population from adult mouse bone marrow possessed a rapid lymphoid-restricted (T, B, and NK) reconstitution capacity in vivo but completely lacked myeloid differentiation potential either in vivo or in vitro. A single Lin(-)IL-7R(+)Thy-1(-)Sca-1loc-Kit(lo) cell could generate at least both T and B cells. These data provide direct evidence for the existence of common lymphoid progenitors in sites of early hematopoiesis.
Kondo
M
M
Department of Pathology, Stanford University School of Medicine, California 94305, USA.
Weissman
I L
IL
Akashi
K
K
D000818:Animals; D000950:Antigens, Ly / Q000032:analysis; D001402:B-Lymphocytes / Q000166:cytology; D002454:Cell Differentiation / Q000502:physiology; D002999:Clone Cells / Q000737:chemistry / Q000166:cytology; D005434:Flow Cytometry; D006412:Hematopoietic Stem Cells / Q000737:chemistry* / Q000166:cytology*; D007201:Indicator Dilution Techniques; D015851:Interleukin-7 / Q000032:analysis; D008565:Membrane Proteins / Q000032:analysis; D008747:Methylcellulose; D051379:Mice; D008810:Mice, Inbred C57BL; D019009:Proto-Oncogene Proteins c-kit / Q000032:analysis; D013601:T-Lymphocytes / Q000166:cytology; D018800:Thy-1 Antigens / Q000032:analysis
D000950:Antigens, Ly; D015851:Interleukin-7; C074650:Ly6a protein, mouse; D008565:Membrane Proteins; D018800:Thy-1 Antigens; D008747:Methylcellulose; D019009:Proto-Oncogene Proteins c-kit