title: The sacB gene cannot be used as a counter-selectable marker in Pasteurella multocida.
abstract: The use of the Bacillus subtilis sacB gene as a counter-selectable marker was assessed in serogroup A and B strains of Pasteurella multocida. Expression ofsacB failed to render any of the strains sensitive to sucrose, indicating that the sacB gene can not be used as a positive selection system in P. multocida.
authors.affiliation: Department of Microbiology, Monash University, Clayton VIC, Australia.
authors.forename: B H
authors.initials: BH
authors.lastname: Jost
authors.forename: P
authors.initials: P
authors.lastname: Homchampa
authors.forename: R A
authors.initials: RA
authors.lastname: Strugnell
authors.forename: B
authors.initials: B
authors.lastname: Adler
mesh_terms: D005819:Genetic Markers; D006602:Hexosyltransferases / Q000187:drug effects / Q000235:genetics* / Q000378:metabolism; D009154:Mutation; D016979:Pasteurella multocida / Q000235:genetics* / Q000378:metabolism; D012641:Selection, Genetic; D013395:Sucrose / Q000378:metabolism / Q000494:pharmacology; D014169:Transformation, Bacterial
chemical_list: D005819:Genetic Markers; D013395:Sucrose; D006602:Hexosyltransferases; C021513:levansucrase