title: Crystallization and preliminary X-ray studies of two serine proteinase inhibitors, BGIA and BGIT, from the seeds of bitter gourd.
abstract: Two serine proteinase inhibitors from seeds of the bitter gourd, BGIA (bitter gourd inhibitor against acidic amino acid-specific proteinase of Streptomyces griseus) and BGIT (bitter gourd trypsin inhibitor), were crystallized for X-ray structure determination. Crystals of BGIA belong to the monoclinic space group C2 with cell dimensions of a = 54.0 A, b = 23.7 A, c = 47.9 A, and beta = 105.4 degrees, and diffracted X-ray up to 1.5 A resolution. Crystals of BGIT belong to the triclinic space group P1 with cell dimensions of a = 22.8 A, b = 23.5 A, c = 28.4 A, alpha = 93.1 degrees, beta = 99.6 degrees, and gamma = 101.0 degrees, giving X-ray diffraction of over 1.2 A resolution. Intensity data of BGIA and BGIT crystals were collected using synchrotron radiation up to 1.7 and 1.4 A, respectively.
authors.affiliation: Graduate School of Science, Hokkaido University, Sapporo, 060, Japan.
authors.forename: H
authors.initials: H
authors.lastname: Kondo
authors.forename: J
authors.initials: J
authors.lastname: Nakanose
authors.forename: A
authors.initials: A
authors.lastname: Nakagawa
authors.forename: I
authors.initials: I
authors.lastname: Tanaka
authors.forename: M
authors.initials: M
authors.lastname: Kimura
authors.forename: G
authors.initials: G
authors.lastname: Funatsu
mesh_terms: D003460:Crystallization; D018360:Crystallography, X-Ray; D019662:Cucurbitaceae / Q000737:chemistry*; D010455:Peptides / Q000737:chemistry* / Q000302:isolation & purification; D010940:Plant Proteins / Q000737:chemistry / Q000302:isolation & purification; D012639:Seeds / Q000737:chemistry; D015842:Serine Proteinase Inhibitors / Q000737:chemistry* / Q000302:isolation & purification; D014361:Trypsin Inhibitors / Q000737:chemistry* / Q000302:isolation & purification
chemical_list: D010455:Peptides; D010940:Plant Proteins; D015842:Serine Proteinase Inhibitors; D014361:Trypsin Inhibitors; C093459:trypsin inhibitor BGIT, Momordica charantia; C070697:BGIA protease inhibitor