Mediation of Sonic hedgehog-induced expression of COUP-TFII by a protein phosphatase.
A Sonic hedgehog (Shh) response element was identified in the chicken ovalbumin upstream promoter-transcription factor II (COUP-TFII) promoter that binds to a factor distinct from Gli, a gene known to mediate Shh signaling. Although this binding activity is specifically stimulated by Shh-N (amino-terminal signaling domain), it can also be unmasked with protein phosphatase treatment in the mouse cell line P19, and induction by Shh-N can be blocked by phosphatase inhibitors. Thus, Shh-N signaling may result in dephosphorylation of a target factor that is required for activation of COUP-TFII-, Islet1-, and Gli response element-dependent gene expression. This finding identifies another step in the Shh-N signaling pathway.
Krishnan
V
V
Department of Cell Biology, Baylor College of Medicine, 1 Baylor Plaza, Houston, TX 77030 USA.
Pereira
F A
FA
Qiu
Y
Y
Chen
C H
CH
Beachy
P A
PA
Tsai
S Y
SY
Tsai
M J
MJ
D000818:Animals; D051837:COUP Transcription Factor II; D051836:COUP Transcription Factors; D002460:Cell Line; D004247:DNA / Q000378:metabolism; D004268:DNA-Binding Proteins / Q000235:genetics* / Q000378:metabolism; D004791:Enzyme Inhibitors / Q000494:pharmacology; D005786:Gene Expression Regulation; D053823:Hedgehog Proteins; D008387:Marine Toxins; D051379:Mice; D019319:Okadaic Acid / Q000494:pharmacology; D010080:Oxazoles / Q000494:pharmacology; D010749:Phosphoprotein Phosphatases / Q000037:antagonists & inhibitors / Q000378:metabolism*; D010766:Phosphorylation; D011401:Promoter Regions, Genetic; D011506:Proteins / Q000235:genetics* / Q000378:metabolism*; D011987:Receptors, Steroid; D015398:Signal Transduction; D015534:Trans-Activators; D014157:Transcription Factors / Q000235:genetics* / Q000378:metabolism
D051837:COUP Transcription Factor II; D051836:COUP Transcription Factors; D004268:DNA-Binding Proteins; D004791:Enzyme Inhibitors; D053823:Hedgehog Proteins; D008387:Marine Toxins; C497587:Nr2f2 protein, mouse; D010080:Oxazoles; D011506:Proteins; D011987:Receptors, Steroid; D015534:Trans-Activators; D014157:Transcription Factors; D019319:Okadaic Acid; C059041:calyculin A; D004247:DNA; D010749:Phosphoprotein Phosphatases