Mutations at codon 249 of p53 gene in human hepatocellular carcinomas from Tongan, China.
Codon 249 (exon 7) of the putative tumor suppressor gene p53 is a mutational hot-spot for hepatocellular carcinoma (HCC) but not other tumors. DNA samples from primary HCC patients from Tongan, an area of high HCC incidence in China (> 40 per 100,000 population), were analyzed for specific mutations in codon 249 of the p53 gene using polymerase chain reaction (PCR)/restriction-digest methods and direct DNA sequencing. Seven of the 21 samples screened were found to have a point mutation at the third base position of codon 249 (AGG to AGT). The result is consistent with previous reports that the G-->T transversion is positively associated with the level of dietary aflatoxin B1 (AFB1) contamination, which has been implicated as one of the risk factors in Tongan area. Of the 7 HCC patients that contained the codon 249 point mutation, one was hepatitis B virus (HBV)-negative. This is only the second documentation of an HCC patient harboring the p53 codon 249 mutation, who was HBV-negative.
Yang
M
M
Department of Biology and Chemistry, City University of Hong Kong, Kowloon, Hong Kong. bhmyang@cityu.edu.hk
Zhou
H
H
Kong
R Y
RY
Fong
W F
WF
Ren
L Q
LQ
Liao
X H
XH
Wang
Y
Y
Zhuang
W
W
Yang
S
S
D000328:Adult; D006528:Carcinoma, Hepatocellular / Q000235:genetics*; D002681:China; D003062:Codon; D015252:Deoxyribonucleases, Type II Site-Specific / Q000378:metabolism; D005260:Female; D016158:Genes, p53; D006514:Hepatitis B Surface Antigens / Q000097:blood; D006801:Humans; D008113:Liver Neoplasms / Q000235:genetics*; D008297:Male; D008875:Middle Aged; D009154:Mutation; D016133:Polymerase Chain Reaction / Q000379:methods; D015183:Restriction Mapping; D012307:Risk Factors; D017422:Sequence Analysis, DNA; D000509:alpha-Fetoproteins / Q000032:analysis
D003062:Codon; D006514:Hepatitis B Surface Antigens; D000509:alpha-Fetoproteins; D015252:Deoxyribonucleases, Type II Site-Specific; C024403:GGCC-specific type II deoxyribonucleases