The effect of sodium tetradecyl sulfate on mobility and antigen detectability of microtubule proteins in sodium dodecyl sulfate-polyacrylamide gel electrophoresis.
Several factors been reported to influence the mobility of polypeptide in sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) including the brand of SDS. Using microtubule proteins from axonemes of Lytechinus pictus and Spisula solidissima sperm and meiotic spindles of Spisula solidissima we demonstrate that the change in mobility was caused by sodium tetradecyl sulfate (STS), a major contaminant of many commercial SDS brands. We also examined the use of sodium tetradecyl sulfate and different SDS brands as a tool in extracting more information from immunoblot studies. Commercial SDS containing contaminants other than sodium tetradecyl sulfate reduced or eliminated the immunosignal from certain polypeptides and the loss of antigenicity could not even be recovered by immunoblot under "renaturing" conditions. It can thus be concluded that STS can be useful in separating and identifying comigrating polypeptides and in detecting additional immunobands in immunoblots.
Hodgkinson
J L
JL
University of Minnesota, Department of Cell Biology and Neuroanatomy, USA.
Steffen
W
W
D000818:Animals; D000941:Antigens / Q000032:analysis; D049872:Bivalvia; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods*; D005260:Female; D015151:Immunoblotting; D008297:Male; D008868:Microtubule Proteins / Q000032:analysis* / Q000737:chemistry; D009865:Oocytes / Q000648:ultrastructure; D012617:Sea Urchins; D012967:Sodium Dodecyl Sulfate; D012981:Sodium Tetradecyl Sulfate / Q000494:pharmacology*; D013094:Spermatozoa / Q000648:ultrastructure
D000941:Antigens; D008868:Microtubule Proteins; D012967:Sodium Dodecyl Sulfate; D012981:Sodium Tetradecyl Sulfate