title: Antibody responses of cows during an outbreak of neosporosis evaluated by indirect fluorescent antibody test and different enzyme-linked immunosorbent assays.
abstract: Serum samples from 70 (33 aborting and 37 non-aborting) dairy cows from a herd in California were analyzed for Neospora caninum antibodies in different laboratories by various serologic assays including enzyme-linked immunosorbent assay (ELISA) with recombinant antigens (Nc4.1 and Nc14.1), kinetic ELISA, whole tachyzoite lysate ELISA, immunostimulating complex (iscom) ELISA, antigen capture competitive inhibition ELISA, and by the indirect fluorescent antibody test. Eighteen percent of pregnant cows in this herd had aborted within 2 mo of the index case. All 70 cows had antibodies to N. caninum by at least 1 of the tests. Antibody levels to N. caninum in aborting cows as a group were higher than in nonaborting cows. However, it was concluded that no serological test could be used to establish definitively that N. caninum caused the abortion in an individual cow.
authors.affiliation: United States Department of Agriculture, Agricultural Research Service, Livestock and Poultry Sciences Institute, Beltsville, Maryland 20705-2350, USA.
authors.forename: J P
authors.initials: JP
authors.lastname: Dubey
authors.forename: M C
authors.initials: MC
authors.lastname: Jenkins
authors.forename: D S
authors.initials: DS
authors.lastname: Adams
authors.forename: M M
authors.initials: MM
authors.lastname: McAllister
authors.forename: R
authors.initials: R
authors.lastname: Anderson-Sprecher
authors.forename: T V
authors.initials: TV
authors.lastname: Baszler
authors.forename: O C
authors.initials: OC
authors.lastname: Kwok
authors.forename: N C
authors.initials: NC
authors.lastname: Lally
authors.forename: C
authors.initials: C
authors.lastname: Björkman
authors.forename: A
authors.initials: A
authors.lastname: Uggla
mesh_terms: D000034:Abortion, Veterinary / Q000453:epidemiology / Q000276:immunology; D000818:Animals; D000913:Antibodies, Protozoan / Q000096:biosynthesis* / Q000097:blood; D000953:Antigens, Protozoan / Q000276:immunology; D002140:California / Q000453:epidemiology; D002417:Cattle; D002418:Cattle Diseases / Q000453:epidemiology* / Q000276:immunology; D003048:Coccidiosis / Q000453:epidemiology* / Q000276:immunology / Q000662:veterinary*; D004196:Disease Outbreaks / Q000706:statistics & numerical data / Q000662:veterinary*; D004797:Enzyme-Linked Immunosorbent Assay / Q000706:statistics & numerical data; D005260:Female; D019084:Fluorescent Antibody Technique, Indirect / Q000706:statistics & numerical data; D018688:Neospora / Q000276:immunology*; D011247:Pregnancy; D015597:Pregnancy Complications, Parasitic / Q000453:epidemiology / Q000276:immunology / Q000662:veterinary; D011336:Probability; D014122:Toxoplasma / Q000276:immunology
chemical_list: D000913:Antibodies, Protozoan; D000953:Antigens, Protozoan