title: One-step purification of nisin A by immunoaffinity chromatography.
abstract: The lantibiotic nisin A was purified to homogeneity by a single-step immunoaffinity chromatography method. An immunoadsorption matrix was developed by direct binding of anti-nisin A monoclonal antibodies to N-hydroxysuccinimide-activated Sepharose. The purification procedure was rapid and reproducible and rendered much higher final yields of nisin than any other described method.
authors.affiliation: Departamento de Nutrición y Bromatología III, Facultad de Veterinaria, Universidad Complutense, Madrid, Spain. anagea@eucmax.sim.ucm.es
authors.forename: A M
authors.initials: AM
authors.lastname: Suárez
authors.forename: J I
authors.initials: JI
authors.lastname: Azcona
authors.forename: J M
authors.initials: JM
authors.lastname: Rodríguez
authors.forename: B
authors.initials: B
authors.lastname: Sanz
authors.forename: P E
authors.initials: PE
authors.lastname: Hernández
mesh_terms: D000900:Anti-Bacterial Agents / Q000276:immunology / Q000302:isolation & purification*; D000911:Antibodies, Monoclonal; D002846:Chromatography, Affinity / Q000379:methods; D005069:Evaluation Studies as Topic; D013294:Lactococcus lactis / Q000737:chemistry; D009561:Nisin / Q000276:immunology / Q000302:isolation & purification*
chemical_list: D000900:Anti-Bacterial Agents; D000911:Antibodies, Monoclonal; D009561:Nisin; C102526:nisin A