Cloning and sequence analysis of another Shiga-like toxin IIe variant gene (slt-IIera) from an Escherichia coli R107 strain isolated from rabbit.
An Escherichia coli R107 strain (O26 serotype) producing a Shiga-like toxin IIe variant (SLT-IIera) was isolated from the mesenteric lymph node of a freshly dead rabbit carcass. The entire structural gene for this SLT-IIera was cloned from chromosomal DNA by PCR using primers based on previously published slt-IIe sequences. Nucleotide sequence analysis indicated that the slt-IIera gene was very similar to slt-IIe (formerly called slt-IIv) from E. coli strains S1191 and 412; five and one nucleotide changes were detected in A and B subunits, respectively, which resulted in changes in amino acid sequences of the corresponding subunits by three and one residues. Recombinant SLT-IIera and SLT-IIe produced using an E. coli host-vector system showed similar cytotoxicity, suggesting that the variations in the structural gene of SLT-IIera have no significant effect on cytotoxic level.
Kim
S H
SH
College of Veterinary Medicine, Gyeongsang National University, Chin-Ju, South Korea.
Cha
I H
IH
Kim
K S
KS
Kim
Y H
YH
Lee
Y C
YC
D000595:Amino Acid Sequence; D000818:Animals; D001427:Bacterial Toxins / Q000737:chemistry / Q000235:genetics* / Q000302:isolation & purification / Q000633:toxicity; D016466:CHO Cells; D002522:Chlorocebus aethiops; D003001:Cloning, Molecular; D006224:Cricetinae; D004768:Enterotoxins / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification / Q000633:toxicity; D004926:Escherichia coli / Q000737:chemistry / Q000145:classification / Q000235:genetics*; D005798:Genes, Bacterial; D014644:Genetic Variation; D008198:Lymph Nodes / Q000382:microbiology; D008969:Molecular Sequence Data; D011817:Rabbits; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D012703:Serotyping; D022641:Shiga Toxin 2; D014709:Vero Cells
D001427:Bacterial Toxins; D004768:Enterotoxins; D022641:Shiga Toxin 2