PCR detection of Clostridium perfringens producing different toxins in faeces of goats.
A polymerase chain reaction (PCR) was used to identify the genes encoding the major toxins of Clostridium perfringens in faeces of goats. When pure cultures of Cl. perfringens types A, B, C, D and E were used as templates in the PCR, amplicons were observed on the agarose gel as bands at approximately the 247 (alpha primers), 1025 (beta primers), 403 (epsilon primers) and 298 (iota primers) bp level of the DNA marker. When used to identify different types of Cl. perfringens in samples artificially spiked with these micro-organisms, the PCR detected as few as 1-1.5 x 10(2) cfu g-1 of the five types of Cl. perfringens tested. The PCR technique allowed the identification and typing of Cl. perfringens strains in faeces of goats, without recourse to other techniques such as the mouse neutralization test.
Uzal
F A
FA
Department of Veterinary Pathology, University of Queensland, Brisbane, Australia.
Plumb
J J
JJ
Blackall
L L
LL
Kelly
W R
WR
D000818:Animals; D001427:Bacterial Toxins / Q000096:biosynthesis*; D003016:Clostridium perfringens / Q000302:isolation & purification* / Q000472:pathogenicity; D005243:Feces / Q000382:microbiology*; D006041:Goats / Q000382:microbiology*; D051379:Mice; D016133:Polymerase Chain Reaction
D001427:Bacterial Toxins