Extracellular matrix modulates mesangial cell apoptosis and mRNA expression of cathepsin-B and tissue transglutaminase.
Mesangial matrix is a dynamic structure which modulates mesangial cell function. Since accumulation of matrix precedes the development of focal glomerulosclerosis, we studied the effect of different matrices on mesangial cell (MC) apoptosis. Suspended mesangial cells became apoptotic in a time dependent manner. Collagen type III did not modulate MC apoptosis when compared to cells grown on plastic. MCs grown on Matrigel, collagen type I and IV showed an increased number of apoptotic cells when compared to MCs grown on plastic. DNA end-labeling further confirmed these observations. MCs grown on Matrigel showed enhanced (P < 0.05) mRNA expression for tissue transglutaminase (TTG) and cathepsin-B. Mesangial cells grown on Matrigel also showed enhanced expression of superoxide dismutase (SOD). We conclude that mesangial cells require attachment to the matrix for their survival and alteration of the quality of matrix modulates mesangial cell apoptosis.
Singhal
P C
PC
Department of Medicine, Long Island Jewish Medical Center, New Hyde Park, New York 11040, USA.
Franki
N
N
Kumari
S
S
Sanwal
V
V
Wagner
J D
JD
Mattana
J
J
D000818:Animals; D017209:Apoptosis / Q000235:genetics / Q000502:physiology; D001672:Biocompatible Materials / Q000494:pharmacology; D002401:Cathepsin B / Q000187:drug effects / Q000235:genetics* / Q000378:metabolism; D002461:Cell Line, Transformed; D004273:DNA, Neoplasm / Q000032:analysis; D005109:Extracellular Matrix / Q000502:physiology*; D015971:Gene Expression Regulation, Enzymologic / Q000187:drug effects; D005920:Glomerular Mesangium / Q000166:cytology*; D051379:Mice; D012333:RNA, Messenger / Q000187:drug effects / Q000235:genetics / Q000378:metabolism*; D013482:Superoxide Dismutase / Q000187:drug effects / Q000235:genetics; D011503:Transglutaminases / Q000187:drug effects / Q000378:metabolism*
D001672:Biocompatible Materials; D004273:DNA, Neoplasm; D012333:RNA, Messenger; D013482:Superoxide Dismutase; D011503:Transglutaminases; D002401:Cathepsin B