title: Histamine and eicosanoid levels in plasma and peripheral blood leucocyte cultures during experimental infection of cattle with bluetongue virus serotype 11.
abstract: Three calves were sensitized with three doses of inactivated BTV-11 UC8 strain and then experimentally infected with the homologous virus. In addition, four BTV-seronegative heifers were also experimentally infected with BTV-11. Granulocyte rich fractions of peripheral blood leucocyte (PBL-GRF) cultures from BTV-sensitized/infected calves and from control unexposed cattle were exposed in vitro with BTV-11. Histamine, leukotriene (LT) C4 and prostaglandin (PG) D2 were assayed in supernatant fluids. Plasma histamine levels increased in BTV-infected heifers from 10.1 +/- 2 ng/ml at Day 0 to 23.1 +/- 6.6 ng/ml at Day 12 following virus exposure. In addition, in this experimental group the concentration of PGF2 alpha (mean 551.97 +/- 243.54 pg/ml) increased significantly (P < or = 0.05) compared with control cattle (mean 467.3 +/- 73.9 pg/ml). Bluetongue virus induced histamine and LTC4 release after in vitro infection of PBL-GRF. Release of LTC4 was significantly (P < or = 0.05) higher in PBL-GRF cultures from sensitized and control animals than in unexposed PBL-GRF cultures. In contrast to these results, PGD2 was not released after BTV infection of PBL-GRF in vitro. The histamine release caused by BTV was virus-specific and mainly mediated by an immunological reaction, since the release was significantly reduced by removal of cell surface immunoglobulins.
authors.affiliation: Department of Pathology, Microbiology and Immunology, School of Veterinary Medicine, University of California, Davis, USA.
authors.forename: A C
authors.initials: AC
authors.lastname: Odeón
authors.forename: S N
authors.initials: SN
authors.lastname: Giri
authors.forename: A
authors.initials: A
authors.lastname: de la Concha-Bermejillo
authors.forename: B I
authors.initials: BI
authors.lastname: Osburn
mesh_terms: D000818:Animals; D001819:Bluetongue / Q000097:blood* / Q000378:metabolism / Q000473:pathology; D001820:Bluetongue virus / Q000145:classification / Q000302:isolation & purification / Q000502:physiology; D002417:Cattle; D002418:Cattle Diseases / Q000097:blood* / Q000378:metabolism / Q000473:pathology; D002478:Cells, Cultured; D015777:Eicosanoids / Q000097:blood* / Q000378:metabolism; D005260:Female; D006632:Histamine / Q000097:blood* / Q000378:metabolism; D007962:Leukocytes / Q000166:cytology / Q000378:metabolism* / Q000821:virology
chemical_list: D015777:Eicosanoids; D006632:Histamine