Tumor specific contrast enhancement study of Mn-metalloporphyrin (ATN-10)--comparison of rat brain tumor model, cytotoxic and vasogenic edema models.
ATN-10, Mn-metalloporphyrin, has been developed as a tumor selective contrast agent for magnetic resonance (MR) imaging. To investigate the tumor specificity of ATN-10, we produced three experimental in vivo models; rat bran tumor (9L glioma) model, vasogenic (cold injury) and cytotoxic brain edema (24-hour MCA occlusion) models. The time course of contrast enhancement was compared after intravenous injection of ATN-10 or Gd-DTPA, measuring the signal intensity of the region of interest. After ATN-10 administration, the 9L glioma model showed early (5 min) and delayed (24 hr-) peak enhancement whereas the cold injury model showed only early enhancement and the 24-hour MCA occlusion model did not show significant enhancement. After Gd-DTPA administration, all three models showed similar pattern of only early enhancement. As a contrast agent for MR imaging, ATN-10 showed different behavior than Gd-DTPA in demonstrating the blood-brain barrier disruption and moreover ATN-10 showed selective enhancement in experimental brain tumors.
Fujimori
H
H
Department of Neurosurgery, University of Tsukuba, Japan.
Matsumura
A
A
Yamamoto
T
T
Shibata
Y
Y
Yoshizawa
T
T
Nakagawa
K
K
Yoshii
Y
Y
Nose
T
T
Sakata
I
I
Nakajima
S
S
D000818:Animals; D001812:Blood-Brain Barrier / Q000502:physiology; D001929:Brain Edema / Q000175:diagnosis*; D001932:Brain Neoplasms / Q000175:diagnosis*; D002470:Cell Survival / Q000502:physiology; D003080:Cold Temperature; D003287:Contrast Media; D003905:Deuteroporphyrins; D004195:Disease Models, Animal; D019786:Gadolinium DTPA; D005910:Glioma / Q000175:diagnosis*; D007205:Indium Radioisotopes; D008279:Magnetic Resonance Imaging / Q000379:methods*; D008297:Male; D051381:Rats; D011916:Rats, Inbred F344
D003287:Contrast Media; D003905:Deuteroporphyrins; D007205:Indium Radioisotopes; C080234:ATN 10; D019786:Gadolinium DTPA