Cytokine production by a new undifferentiated human thyroid carcinoma cell line, FB-1.
A human anaplastic thyroid cancer cell line FB-1, derived from a 68-yr-old woman who underwent surgery for anaplastic thyroid cancer, has been established. The spindlelike cells have been proliferating stably for more than 2 yr. Karyotype analysis shows many abnormalities and many marker chromosomes have been observed. Heterotransplant of FB-1 cells into severe combined immunodeficient mice has resulted in rapidly growing tumors classified as anaplastic carcinomas, although 50% have shown areas with a trabecular pattern. FB-1 cells failed to express messenger RNA for thyroglobulin; TSH-receptor; thyroperoxidase, and placental angiogenic growth factor. Conversely, PAX8 and thyroid transcription factor 1, whose expression is thyroid specific, was kept in an FB-1 cell line at a level comparable with that observed in normal thyroid tissue. In addition, the present cell line expressed high levels of messenger RNA for high-mobility group proteins (Y) and -C. The in vitro study revealed that FB-1 cells are able to produce high levels of interleukin (IL)-8 and medium amount of IL-6, whereas no release of IL-1-alpha, IL-1-beta, and IL-4 was observed. No modulation of cell proliferation and DNA synthesis in FB-1 cells has been observed after the addition of exogenous IL-6.
Fiore
L
L
Dipartimento di Oncologia, Università degli Studi di Pisa, Italy.
Pollina
L E
LE
Fontanini
G
G
Casalone
R
R
Berlingieri
M T
MT
Giannini
R
R
Pacini
F
F
Miccoli
P
P
Toniolo
A
A
Fusco
A
A
Basolo
F
F
D000368:Aged; D000818:Animals; D015415:Biomarkers; D015197:Carcinogenicity Tests; D002277:Carcinoma / Q000378:metabolism* / Q000473:pathology; D002454:Cell Differentiation / Q000502:physiology; D002455:Cell Division / Q000187:drug effects; D002469:Cell Separation; D002471:Cell Transformation, Neoplastic; D016207:Cytokines / Q000096:biosynthesis* / Q000494:pharmacology; D005260:Female; D006133:Growth Substances / Q000494:pharmacology; D006801:Humans; D007621:Karyotyping; D051379:Mice; D016513:Mice, SCID; D012333:RNA, Messenger / Q000378:metabolism; D013961:Thyroid Gland / Q000166:cytology; D013964:Thyroid Neoplasms / Q000378:metabolism* / Q000473:pathology; D014407:Tumor Cells, Cultured / Q000378:metabolism*
D015415:Biomarkers; D016207:Cytokines; D006133:Growth Substances; D012333:RNA, Messenger