Anti-interleukin-10 antibodies in patients with chronic active Epstein-Barr virus infection.
The Epstein-Barr virus (EBV) genome encodes a protein in its BamHI C restriction fragment rightward open-reading frame-1 (designated BCRF1 or viral interleukin-10 [vIL-10]) that shares protein homology and biologic properties with human IL-10. Several EBV disorders are characterized by prolonged active EBV infection. Because continued EBV replication could allow for increased vIL-10, ELISA and immunoprecipitation were used to determine whether vIL-10 expression during chronic active EBV infection resulted in vIL-10 and IL-10 antibodies. IL-10 antibodies were assayed in patients diagnosed with chronic and acute infectious mononucleosis (CIM, AIM), nasopharyngeal carcinoma (NPC), and EBV-associated lymphoproliferative disease (LPD), as well as from healthy organ transplant patients and EBV-negative or EBV-positive persons. Whether anti-IL-10 antibodies could inhibit IL-10 biologic activity was determined. vIL-10 antibodies were found in CIM, NPC, and LPD patients and antibodies reactive to IL-10 were found in CIM patients. One CIM patient had IL-10 antibodies that neutralized IL-10 bioactivity in vitro.
Tanner
J E
JE
Children's Hospital of Eastern Ontario Research Center, Department of Pediatrics, University of Ottawa, Canada.
Diaz-Mitoma
F
F
Rooney
C M
CM
Alfieri
C
C
D000818:Animals; D000914:Antibodies, Viral / Q000097:blood / Q000276:immunology*; D016466:CHO Cells; D002460:Cell Line; D002908:Chronic Disease; D006224:Cricetinae; D004797:Enzyme-Linked Immunosorbent Assay; D006566:Herpesviridae Infections / Q000276:immunology*; D004854:Herpesvirus 4, Human / Q000235:genetics / Q000276:immunology*; D006801:Humans; D007244:Infectious Mononucleosis / Q000276:immunology*; D016753:Interleukin-10 / Q000032:analysis / Q000235:genetics / Q000276:immunology*; D008232:Lymphoproliferative Disorders / Q000276:immunology / Q000821:virology; D009303:Nasopharyngeal Neoplasms / Q000276:immunology / Q000821:virology; D009500:Neutralization Tests; D016377:Organ Transplantation; D011233:Precipitin Tests; D011994:Recombinant Proteins / Q000276:immunology; D014409:Tumor Necrosis Factor-alpha / Q000032:analysis; D014764:Viral Proteins / Q000235:genetics / Q000276:immunology* / Q000378:metabolism
D000914:Antibodies, Viral; C066460:BCRF1 protein, Human herpesvirus 4; D011994:Recombinant Proteins; D014409:Tumor Necrosis Factor-alpha; D014764:Viral Proteins; D016753:Interleukin-10