title: Nitecapone is of benefit to functional performance in experimental heart transplantation.
abstract: In heart transplantation, global ischemia of a graft is followed by reperfusion injury. The formation of oxygen free radicals induces arrhythmias and impairs functional recovery of the graft. This study was executed to evaluate the effect of the new antioxidant, nitecapone, on ischemia-reperfusion injury in heart transplantation in rats. Donor hearts were perfused and stored at +4 degrees C for 2 h in either Ringer's solution in the control group (C-group, n = 26) or Ringer's solution with nitecapone (NC) added (NC-group, n = 18). The donor aorta was anastomosed to the recipient's abdominal aorta and the pulmonary artery to the recipient's inferior vena cava. The grafts were classified into three categories based on the functional recovery. The rats in both groups were killed at 10, 30, or 60 min after release of the aortic clamp. Tissue samples for chemiluminescence were obtained from the left ventricle, the right ventricle, and the septum of the heart. All grafts in the NC-group (18/18) began beating after release of the aortic clamp, whereas only 50% (13/26) of the grafts in the C-group recovered (P < 0.0004). Chemiluminescence analysis showed lipid peroxidation values to be higher in the C-group than the NC-group up to 1 h after reperfusion. Also, the right ventricle samples showed lower chemiluminescence values in the NC-group than in the C-group. In conclusion, our results do not support the theory that different regions of the heart have different vulnerability to ischemia-reperfusion injuries. Nitecapone has a beneficial effect on the preservation of the grafts in terms of functional recovery.
authors.affiliation: Department of Thoracic and Cardiovascular Surgery, Helsinki University Central Hospital, Finland.
authors.forename: A E
authors.initials: AE
authors.lastname: Vento
authors.forename: O J
authors.initials: OJ
authors.lastname: Rämö
authors.forename: A T
authors.initials: AT
authors.lastname: Nemlander
authors.forename: E
authors.initials: E
authors.lastname: Nissinen
authors.forename: A
authors.initials: A
authors.lastname: Holopainen
authors.forename: S P
authors.initials: SP
authors.lastname: Mattila
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000494:pharmacology*; D002396:Catechols / Q000494:pharmacology*; D005260:Female; D016027:Heart Transplantation; D008163:Luminescent Measurements; D008297:Male; D015428:Myocardial Reperfusion Injury / Q000188:drug therapy*; D010422:Pentanones / Q000494:pharmacology*; D051381:Rats; D017208:Rats, Wistar; D016277:Ventricular Function, Left
chemical_list: D000975:Antioxidants; D002396:Catechols; D010422:Pentanones; C057364:nitecapone