title: [Effects of SAD on cytogenetics of mouse embryonic cells and its embryo development].
abstract: We report here that SAD may not only suppress transcription activity of rRNA genes, but also increase SCE frequency of mouse embryonic cells, leading to bloching of the embryo development and fetal death, and finally significant decrease of birth percentage of the new born offsprings. Ata concentration of SAD in 10(-5)mol/L, the transcription activity of rRNA genes was decreased to 15% of the normal level, and the SCE frequency was increased doubly, leading to that the birth percentage was decreased to 50% of normal one. When SAD concentration of 10(-3)mol/L was used the transcription activity of rRNA genes was completely suppressed and SCE frequency was increased to as 4.8 times high as normal, leading to that the birth percentage dropped to 4% from 100%. Our results indicates that both the cytogenetic effects and the inhibition of the embryonic cells are directly proportional to the SAD concentration used. The relationship between the change of both the rRNA gene transcription and SCE frequency and the degree of embryo development are also discussed preliminarily.
authors.affiliation: Institute of Genetics Chinese Academy of Sciences, Beijing.
authors.forename: Y
authors.initials: Y
authors.lastname: Wang
authors.forename: Y
authors.initials: Y
authors.lastname: Yan
authors.forename: Y
authors.initials: Y
authors.lastname: Miao
authors.forename: T
authors.initials: T
authors.lastname: Zhang
authors.forename: R
authors.initials: R
authors.lastname: He
mesh_terms: D000447:Aldehydes / Q000633:toxicity*; D000818:Animals; D005314:Embryonic and Fetal Development / Q000187:drug effects*; D005260:Female; D051379:Mice; D009697:Nucleolus Organizer Region / Q000187:drug effects; D011247:Pregnancy; D012335:RNA, Ribosomal / Q000187:drug effects; D012854:Sister Chromatid Exchange / Q000187:drug effects; D013451:Sulfonic Acids / Q000633:toxicity
chemical_list: D000447:Aldehydes; D012335:RNA, Ribosomal; D013451:Sulfonic Acids