The 1997 Lindberg Award. Effects of burn injury on bone and growth in a mouse model.
Bone growth and remodeling are inhibited by severe burns in adult and pediatric patients, resulting in alterations in linear growth, bone mass, osteoporosis, and increased risk for pathologic fractures. This study of a mouse model of burn injury showed skeletal changes similar to those reported in patients with burn injuries. Baseline, control, sham, and burned mice were injected with fluorescent markers calcein and tetracycline for histomorphometric analysis. Total femur dry and ash weights and total calcium content were significantly lower 10 days after burn injury compared with sham and control animals. There also were decreases in the percentage of fluorochrome-labeled bone surfaces and bone formation rates in the burn-injured mice compared with control and sham mice; however, there were no differences in the mineral apposition rates. This model now provides an opportunity to examine cellular and molecular mechanisms contributing to skeletal pathology in a well-defined burn injury model.
Edelman
L S
LS
Department of Surgery, University of Utah School of Medicine, Salt Lake City 84132, USA.
Shao
W
W
Miller
S
S
Bowman
B
B
Morris
S E
SE
Shelby
J
J
D000818:Animals; D016723:Bone Remodeling / Q000502:physiology*; D001842:Bone and Bones / Q000293:injuries*; D002056:Burns / Q000150:complications / Q000503:physiopathology*; D004195:Disease Models, Animal; D005269:Femur / Q000473:pathology; D005452:Fluoresceins / Q000008:administration & dosage; D005456:Fluorescent Dyes / Q000008:administration & dosage; D006801:Humans; D051379:Mice; D013752:Tetracycline / Q000008:administration & dosage
D005452:Fluoresceins; D005456:Fluorescent Dyes; D013752:Tetracycline; C007740:fluorexon