title: Picosecond to hour time scale dynamics of a "three finger" toxin: correlation with its toxic and antigenic properties.
abstract: Toxin alpha from Naja nigricollis (61 amino acids, four disulfide bridges) belongs to the "three finger" fold family, which contains snake toxins with various biological activities and nontoxic proteins from different origins. In this paper, we report an extensive 1H and 15N NMR study of the dynamics of toxin alpha in solution. 15N relaxation, 1H off-resonance ROESY, and H-D exchange experiments allowed us to probe picosecond to hour motions in the protein. Analysis of these NMR measurements demonstrates that toxin alpha exhibits various time scale motions, i.e., particularly large amplitude picosecond to nanosecond motions at the tips of the loops, observable microsecond to millisecond motions around two disulfide bridges, second time scale motions around the C-N bonds of asparagine and glutamine side chains which are more or less rapid depending on their amino acid solvent accessibility, and minute to hour motions in the beta-sheet structure. The less well-defined regions of toxin alpha solution structures are subject to important picosecond to nanosecond motions. The toxic site is organized around residues belonging to the rigid core of the molecule but also comprises residues exhibiting dynamics on various time scales. The Malpha1 epitope is subject to large picosecond to millisecond motions, which are probably modified by the interaction with the antibody. This phenomenon could be linked to the neutralizing properties of the antibody.
authors.affiliation: Département d'Ingénierie et d'Etudes des Protéines, CEA, Saclay, 91191 Gif-sur-Yvette Cedex, France.
authors.forename: M
authors.initials: M
authors.lastname: Guenneugues
authors.forename: P
authors.initials: P
authors.lastname: Drevet
authors.forename: S
authors.initials: S
authors.lastname: Pinkasfeld
authors.forename: B
authors.initials: B
authors.lastname: Gilquin
authors.forename: A
authors.initials: A
authors.lastname: Ménez
authors.forename: S
authors.initials: S
authors.lastname: Zinn-Justin
mesh_terms: D000818:Animals; D016880:Anisotropy; D000906:Antibodies / Q000276:immunology; D001216:Asparagine / Q000737:chemistry; D001665:Binding Sites; D003039:Cobra Neurotoxin Proteins / Q000737:chemistry* / Q000276:immunology / Q000633:toxicity; D003903:Deuterium; D004220:Disulfides / Q000737:chemistry; D000939:Epitopes / Q000276:immunology; D005973:Glutamine / Q000737:chemistry; D007700:Kinetics; D008958:Models, Molecular; D009587:Nitrogen Isotopes; D019906:Nuclear Magnetic Resonance, Biomolecular; D011487:Protein Conformation; D017433:Protein Structure, Secondary; D017434:Protein Structure, Tertiary; D012984:Software
chemical_list: D000906:Antibodies; D003039:Cobra Neurotoxin Proteins; D004220:Disulfides; D000939:Epitopes; D009587:Nitrogen Isotopes; D005973:Glutamine; C025154:alpha-cobratoxin; D001216:Asparagine; D003903:Deuterium