A strong inhibitor of gene expression in the 5' untranslated region of the pollen-specific LAT59 gene to tomato.
Promoter sequences that direct pollen-specific expression have been previously identified in the LAT59 (for late anther tomato) gene. Here, we show that the LAT59 sequences encoding the 5' untranslated region inhibit expression of reporter genes by > 20-fold in transient expression experiments and up to 300-fold after stable transformation. Inhibition occurred in somatic cells as well as in pollen. Our results indicate that the inhibitor still functions after pollen germination and therefore does not modulate the level of the LAT59 protein during pollen development. The presence of the leader sequence dramatically decreased mRNA accumulation but without affecting translation rate and mRNA stability. We believe that the leader inhibits transcription. We mapped the inhibitor to a region in the leader that coincides with a putative stem-loop and present evidence that this stem-loop participates in inhibition.
Curie
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C
Plant Gene Expression Center, United States Department of Agriculture, Agricultural Research Service, Albany, California, USA.
McCormick
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S
D018506:Gene Expression Regulation, Plant / Q000235:genetics*; D018525:Germination; D018551:Solanum lycopersicum / Q000235:genetics*; D010957:Plasmids; D011058:Pollen / Q000235:genetics*; D014158:Transcription, Genetic / Q000235:genetics; D014170:Transformation, Genetic