Preferential cytotoxicity to tumor cells of 3,5-diprenyl-4-hydroxycinnamic acid (artepillin C) isolated from propolis.
A tumoricidal substance was isolated from Brazilian propolis as guided by cytotoxicity assay on HuH 13 (human hepatocellular carcinoma) cell and was characterized to be 3-[4-hydroxy-3,5-bis (3-methyl-2-butenyl) phenyl]-2-propenoic acid (3,5-diprenyl-4-hydroxycinnamic acid (artepillin C)). It exhibited preferential cytotoxicity to tumor cells cultured in vitro. The cytotoxicity observed seemed to be partly attributable to apoptosis-like DNA fragmentation. The compound showed anti-tumor activity more effective than that of 5-fluorouracil to transplantable human tumor cell lines when tested on histoculture drug response assay system.
Matsuno
T
T
National Institute of Health, Tokyo, Japan.
Jung
S K
SK
Matsumoto
Y
Y
Saito
M
M
Morikawa
J
J
D000970:Antineoplastic Agents / Q000633:toxicity*; D004847:Epithelial Cells / Q000187:drug effects; D005472:Fluorouracil / Q000633:toxicity; D018922:HL-60 Cells; D006801:Humans; D010666:Phenylpropionates / Q000633:toxicity*; D011429:Propolis / Q000032:analysis*; D014407:Tumor Cells, Cultured / Q000187:drug effects
D000970:Antineoplastic Agents; D010666:Phenylpropionates; C102259:artepillin C; D011429:Propolis; D005472:Fluorouracil