TCRV and TCRJ gene usage in MBP responding T cells from (B10.PL x PL/J)F1 mice is biased towards that of B10.PL mice.
We analyzed myelin basic protein (MBP) specific T cell hybridoma clones from (B10.PL x PL/J)F1 mice. MBP-reacting T cell hybridomas from F1 mice preferentially expressed B10.PL TcraV2.3 (53%) and B10.PL TcraV4.2 (13%) with minor expression of TcraV4.4 (13%) gene segments. A dominant expression of TcrbV8.2 (73%) accompanying with TcrbV8.1 (20%) and TcrbV13 (7%) gene segments have been identified in these MBP-reacting T cell hybridomas from F1 mice. There was less restrictive but non-random usage of the TcraJ and TcrbJ gene segments. Overall, the MBP-reacting T cell hybridomas from (B10.PL x PL/J)F1 mice were dominated by the MBP-reacting T cell pattern seen in B10.PL mice.
Cheng
K C
KC
Division of Biology, California Institute of Technology, Pasadena 91125, USA.
Chiang
H J
HJ
Wang
K
K
Krug
M S
MS
Yoo
T J
TJ
Hood
L
L
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003433:Crosses, Genetic; D005434:Flow Cytometry; D005786:Gene Expression Regulation / Q000276:immunology; D015329:Gene Rearrangement, T-Lymphocyte / Q000276:immunology*; D019672:Genes, T-Cell Receptor / Q000276:immunology*; D006825:Hybridomas; D051379:Mice; D008810:Mice, Inbred C57BL; D008969:Molecular Sequence Data; D005810:Multigene Family; D004676:Myelin Basic Protein / Q000235:genetics* / Q000276:immunology / Q000378:metabolism; D051381:Rats; D016693:Receptors, Antigen, T-Cell, alpha-beta / Q000096:biosynthesis / Q000235:genetics; D013045:Species Specificity; D013601:T-Lymphocytes / Q000378:metabolism*
D004676:Myelin Basic Protein; D016693:Receptors, Antigen, T-Cell, alpha-beta