Immunohistochemical and RT-PCR detection of Na+-dependent inorganic phosphate cotransporter (NaPi-2) in rat brain.
Expression of a renal Na+-dependent inorganic phosphate (Pi) cotransporter (NaPi-2) was studied in rat forebrain with reverse transcription and polymerase chain reaction (RT-PCR) and immunohistochemistry. RT-PCR analysis for total RNA from whole brain and sequencing of the PCR products showed expression of NaPi-2 mRNA in the brain. Immunohistochemical analysis revealed NaPi-2 staining in many nonpyramidal neurons of all six layers throughout neocortical areas and in neurons of proisocortical and periallocortical areas. NaPi-2-immunoreactive neurons were also detectable in the piriform cortex, hippocampal formation, caudate-putamen, amygdaloid nuclei and lateral geniculate nucleus. Furthermore, NaPi-2 staining was shown in ependymal cells and microvascular endothelial cells. The present results suggest that NaPi-2 is synthesized within the brain and involved in maintaining Pi homeostasis of certain neurons and/or the entire brain.
Hisano
S
S
Department of Anatomy, School of Medicine, University of Tokushima, Japan. hisano@basic.med.tokushima-u.ac.jp
Haga
H
H
Li
Z
Z
Tatsumi
S
S
Miyamoto
K I
KI
Takeda
E
E
Fukui
Y
Y
D000818:Animals; D001921:Brain / Q000166:cytology / Q000378:metabolism*; D002352:Carrier Proteins / Q000378:metabolism*; D004027:Diencephalon / Q000378:metabolism; D007150:Immunohistochemistry; D008297:Male; D009419:Nerve Tissue Proteins / Q000378:metabolism*; D009474:Neurons / Q000378:metabolism; D010710:Phosphates / Q000378:metabolism*; D016133:Polymerase Chain Reaction / Q000379:methods*; D051381:Rats; D017208:Rats, Wistar; D012964:Sodium / Q000502:physiology*; D013687:Telencephalon / Q000378:metabolism; D014158:Transcription, Genetic
D002352:Carrier Proteins; D009419:Nerve Tissue Proteins; D010710:Phosphates; D012964:Sodium