Bile acid kinetics in man studied by radio thin-layer chromatography and densitometry coupling.
A method based on coupling of the techniques of radioscanning a TLC plate and densitometry has been developed for the determination of pool sizes and fractional turnover rate of bile acids in man after intraduodenal administration of 14C-labelled acid. The validity of the method has been checked by comparison of the results obtained with those of an enzymatic spectrophotometric analysis, and a measurement of the radioactivity by liquid scintillation counting, after elution of the separated bile acid from a TLC plate. Advantages of the proposed method over the previous one include a reduced number of manipulations, the possibility of automation, a better reproducibility, and the possibility of elaborating the radiometric data obtained for the primary bile acid for better characterising its metabolism inside the enterohepatic circulation.
Tonelli
D
D
Dipartimento di Chimica Fisica ed Inorganica, Università di Bologna, Italy.
Gattavecchia
E
E
Mazzella
G
G
Roda
A
A
D002250:Carbon Radioisotopes; D019826:Cholic Acid; D002793:Cholic Acids / Q000378:metabolism / Q000493:pharmacokinetics*; D002855:Chromatography, Thin Layer; D003720:Densitometry; D003840:Deoxycholic Acid / Q000378:metabolism / Q000493:pharmacokinetics*; D006801:Humans; D011865:Radioisotope Dilution Technique; D015203:Reproducibility of Results
D002250:Carbon Radioisotopes; D002793:Cholic Acids; D003840:Deoxycholic Acid; D019826:Cholic Acid