Effect of heparin on cell proliferation: lack of correlation with heparin binding sites on cell membrane.
In this study an attempt was made to correlate the in-vitro anti-proliferative effect of heparin with the heparin binding on the cell surface. Cells with different sensitivities to the anti-proliferative effect of heparin (BHK-21, FAO, SMC, BAEC, A-431, V-79, and skin fibroblasts) were incubated with [3H]heparin either in the presence or in the absence of unlabelled heparin. A saturable binding was found only in BHK-21, FAO, SMC, BAEC and V-79. Scatchard analysis revealed the presence of a single class of binding sites. The binding of [3H] heparin was efficiently displaced by unlabelled heparin, pentosan polysulfate and low-molecular-weight heparin, but not by dermatan sulfate. Although the sensitivity to the anti-proliferative effect of heparin varied considerably among the cell types (BHK-21 > SMC, FAO > BAEC > V-79), there was no correlation between the reduction of proliferation of these cells and either their heparin binding capacity or the number of binding sites per cell.
Tiozzo
R
R
Department of Biomeolical Sciences, University of Modena, Italy.
Reggiani
D
D
Croce
M A
MA
Calandra
S
S
Osima
B
B
Bianchini
P
P
D000818:Animals; D001665:Binding Sites; D002455:Cell Division / Q000187:drug effects*; D002462:Cell Membrane / Q000378:metabolism; D002478:Cells, Cultured; D006224:Cricetinae; D003412:Cricetulus; D003871:Dermatan Sulfate / Q000378:metabolism; D005343:Fibrinolytic Agents / Q000378:metabolism* / Q000494:pharmacology; D005347:Fibroblasts / Q000378:metabolism; D006493:Heparin / Q000378:metabolism* / Q000494:pharmacology; D006801:Humans; D008114:Liver Neoplasms, Experimental / Q000378:metabolism; D009130:Muscle, Smooth / Q000378:metabolism; D051381:Rats
D005343:Fibrinolytic Agents; D003871:Dermatan Sulfate; D006493:Heparin