title: Parvovirus B19 quiescence during the course of human immunodeficiency virus infection in persons with hemophilia.
abstract: To detect and characterize parvovirus B19 infection during the course of progressive immune deficiency from human immunodeficiency virus (HIV), ten subjects enrolled in the Multicenter Hemophilia Cohort Study were followed for 6.4 to 15 years from HIV seroconversion through extreme immune deficiency. Four to five sera or plasma samples from each subject, collected at predetermined CD4+ lymphocyte levels, were tested for immunoglobulin G (IgG) and M (IgM) B19 antibodies and DNA. All 42 samples were positive for B19 IgG antibodies, and three were weakly positive for IgM antibodies. Only one sample, collected coincident with HIV seroconversion, was unequivocally positive for B19 DNA. No persistent hematologic adverse effects of B19 infection were observed. Thus, although B19 IgG antibodies are highly prevalent among HIV-infected persons with hemophilia or related disorders, B19 viremia and its hematologic consequences were not detected, even with severe depletion of CD4+ lymphocytes. If primary B19 infection occurs after immune deficiency, however, the consequences may be more adverse.
authors.affiliation: Viral Epidemiology Branch, National Cancer Institute, Rockville, Maryland, USA. jg8s@nih.gov
authors.forename: J J
authors.initials: JJ
authors.lastname: Goedert
authors.forename: D D
authors.initials: DD
authors.lastname: Erdman
authors.forename: B A
authors.initials: BA
authors.lastname: Konkle
authors.forename: T J
authors.initials: TJ
authors.lastname: Török
authors.forename: M M
authors.initials: MM
authors.lastname: Lederman
authors.forename: D
authors.initials: D
authors.lastname: Kleinert
authors.forename: T
authors.initials: T
authors.lastname: Mandalaki
authors.forename: C M
authors.initials: CM
authors.lastname: Kessler
authors.forename: L J
authors.initials: LJ
authors.lastname: Anderson
authors.forename: N L
authors.initials: NL
authors.lastname: Luban
mesh_terms: D000293:Adolescent; D000328:Adult; D000914:Antibodies, Viral / Q000032:analysis; D018791:CD4 Lymphocyte Count; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology; D002648:Child; D002675:Child, Preschool; D015331:Cohort Studies; D004279:DNA, Viral / Q000032:analysis; D015658:HIV Infections / Q000150:complications* / Q000276:immunology / Q000821:virology; D015497:HIV-1; D006467:Hemophilia A / Q000150:complications* / Q000276:immunology / Q000821:virology; D006801:Humans; D016867:Immunocompromised Host; D007074:Immunoglobulin G / Q000032:analysis; D007075:Immunoglobulin M / Q000032:analysis; D008875:Middle Aged; D010322:Parvoviridae Infections / Q000150:complications* / Q000276:immunology / Q000821:virology; D016732:Parvovirus B19, Human / Q000235:genetics / Q000276:immunology / Q000302:isolation & purification*; D016133:Polymerase Chain Reaction; D011446:Prospective Studies; D014766:Viremia / Q000150:complications
chemical_list: D000914:Antibodies, Viral; D004279:DNA, Viral; D007074:Immunoglobulin G; D007075:Immunoglobulin M