title: Limited antigenic variation among recent infectious bursal disease virus isolates from France.
abstract: Seven neutralizing monoclonal antibodies (Mabs) to infectious bursal disease virus (IBDV) were used in an antigen-capture ELISA for the antigenic characterization of 58 IBDV isolates obtained in France since 1989. Fifty-six isolates exhibited an antigenic profile which was different from reference strain Faragher 52/70, and similar to French very virulent IBDV strain 89,163 (no binding of two Mabs). Two strains (3.4%), isolated in 1991 and 1994, showed additional antigenic modifications resulting in suppressed or reduced binding activity of three other Mabs. The two atypical viruses were not re-identified in field samples subsequently collected, hence showing that antigenic variants of IBDV do not tend to replace the antigenically dominant 89,163-like strains that have prevailed in France since 1989.
authors.affiliation: Centre National d'Etudes Vétérinaires et Alimentaires (CNEVA), Ploufragan, France.
authors.forename: N
authors.initials: N
authors.lastname: Eterradossi
authors.forename: G
authors.initials: G
authors.lastname: Rivallan
authors.forename: D
authors.initials: D
authors.lastname: Toquin
authors.forename: M
authors.initials: M
authors.lastname: Guittet
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D000914:Antibodies, Viral / Q000276:immunology; D000940:Antigenic Variation; D000956:Antigens, Viral / Q000276:immunology*; D018175:Birnaviridae Infections / Q000821:virology; D002645:Chickens; D004797:Enzyme-Linked Immunosorbent Assay; D005602:France; D007243:Infectious bursal disease virus / Q000276:immunology* / Q000302:isolation & purification; D051379:Mice; D008807:Mice, Inbred BALB C; D015678:Viral Structural Proteins / Q000276:immunology*
chemical_list: D000911:Antibodies, Monoclonal; D000914:Antibodies, Viral; D000956:Antigens, Viral; C082513:VP2 protein, infectious bursal disease virus; D015678:Viral Structural Proteins