The order and transcriptional orientation of the human COL13A1 and P4HA genes on chromosome 10 long arm determined by high-resolution FISH.
The genes for type XIII collagen (COL13A1) and prolyl 4-hydroxylase (P4HA) were previously assigned to human chromosome 10q by radioactive in situ hybridization. Here we have applied fluorescence in situ hybridization combined with targets representing different levels of resolution to determine, first, the order of these genes along chromosome 10; second, their transcriptional orientation; and third, the distance between these genes. The order along the chromosome was determined to be centromere-COL13A1-P4HA-telomere using mechanically stretched chromosomes. By combining the data from stretched chromosomes and interphase nuclei, we found that the transcriptional orientation were tail to tail (COL13A1 3'-3' P4HA). The distance between these genes was measured by fiber FISH to be approximately 550 kb.
Horelli-Kuitunen
N
N
Department of Clinical Chemistry and Biomedicine, Helsinki University, Finland.
Kvist
A P
AP
Helaakoski
T
T
Kivirikko
K
K
Pihlajaniemi
T
T
Palotie
A
A
D002503:Centromere / Q000235:genetics; D002879:Chromosomes, Human, Pair 10; D006801:Humans; D017404:In Situ Hybridization, Fluorescence / Q000379:methods*; D011393:Procollagen-Proline Dioxygenase / Q000235:genetics*; D016615:Telomere / Q000235:genetics; D014158:Transcription, Genetic
D011393:Procollagen-Proline Dioxygenase