Zeta, a novel class of glutathione transferases in a range of species from plants to humans.
Sequence alignment and phylogenetic analysis has identified a new subgroup of glutathione S-transferase (GST)-like proteins from a range of species extending from plants to humans. This group has been termed the Zeta class. An atomic model of the N-terminal domain suggests that the members of the Zeta class have a similar structure to that of other GSTs, binding glutathione in a similar orientation in the G site. Recombinant human GSTZ1-1 has been expressed in Escherichia coli and characterized. The protein is a dimer composed of 24.2 kDa subunits and has minimal glutathione-conjugating activity with ethacrynic acid and 7-chloro-4-nitrobenz-2-oxa-1, 3-diazole. Although low in comparison with other GSTs, GSTZ1-1 has glutathione peroxidase activity with t-butyl and cumene hydroperoxides. The members of the Zeta class have been conserved over a long evolutionary period, suggesting that they might have a role in the metabolism of a compound that is common in many living cells.
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Molecular Genetics Group, John Curtin School of Medical Research, Australian National University, GPO Box 34, Canberra, ACT 2601, Australia.
Baker
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Chelvanayagam
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Jermiin
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D000595:Amino Acid Sequence; D000818:Animals; D001665:Binding Sites; D003001:Cloning, Molecular; D017124:Conserved Sequence / Q000235:genetics; D019281:Dimerization; D019143:Evolution, Molecular; D005978:Glutathione / Q000378:metabolism; D005979:Glutathione Peroxidase / Q000378:metabolism; D005982:Glutathione Transferase / Q000032:analysis / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D006801:Humans; D008958:Models, Molecular; D008969:Molecular Sequence Data; D010545:Peroxides / Q000378:metabolism; D010802:Phylogeny; D010944:Plants / Q000201:enzymology; D011994:Recombinant Proteins / Q000737:chemistry; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D013379:Substrate Specificity; D014018:Tissue Distribution
D010545:Peroxides; D011994:Recombinant Proteins; D005979:Glutathione Peroxidase; D005982:Glutathione Transferase; D005978:Glutathione