Relaxation of insulin-like growth factor-II gene imprinting in human gynecologic tumors.
To test for the existence of genomic imprinting in human gynecologic tumors, we analyzed the allelic expression of human insulin-growth factor-II (IGF-II) genes. Genomic imprinting is the parental allele-specific expressions of genes, and recently imprinting of IGF-II gene has demonstrated that parental IGF-II was monoallelically expressed. To study whether IGF-II gene imprinting occurs in human gynecologic tumors, we examined allele-specific expression using an ApaI polymorphism in the 3' untranslated region of IGF-II gene exon 9. We used 19 gynecologic tumor cell lines, and 66 human gynecologic tumors. Four of 19 cell lines (21%) were informative, and three of these four cell lines (75%) revealed loss of imprinting (LOI). For gynecologic tumors, 24 of 66 were informative (36%), and 5 of the 24 (21%) had LOI. We have reported here that the IGF-II gene is expressed biallelically in some gynecologic tumors. We suggest that LOI of the IGF-II gene is involved in the development of some gynecologic tumors.
Yaginuma
Y
Y
Department of Obstetrics and Gynecology, Asahikawa Medical College, Japan.
Nishiwaki
K
K
Kitamura
S
S
Hayashi
H
H
Sengoku
K
K
Ishikawa
M
M
D000230:Adenocarcinoma / Q000235:genetics; D000483:Alleles; D015342:DNA Probes; D004273:DNA, Neoplasm; D016889:Endometrial Neoplasms / Q000235:genetics; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D005833:Genital Neoplasms, Female / Q000235:genetics*; D018392:Genomic Imprinting; D006801:Humans; D007335:Insulin-Like Growth Factor II / Q000235:genetics*; D010051:Ovarian Neoplasms / Q000235:genetics; D012334:RNA, Neoplasm; D014407:Tumor Cells, Cultured; D002583:Uterine Cervical Neoplasms / Q000235:genetics
D015342:DNA Probes; D004273:DNA, Neoplasm; D012334:RNA, Neoplasm; D007335:Insulin-Like Growth Factor II