Separation of sialoglycoprotein fractions having influenza virus-receptor activity from human meconium and some of their chemical properties.
Three sialoglycoprotein fractions having reactivities against influenza A and B viruses (IV-A and IV-B) and influenza virus-hemagglutinin (IV-HA), were obtained by gel filtration using a Sephacryl S-300 column and an Asahipak GS-710 HPLC column from water-soluble fraction prepared from human meconium (ME-WSF). Molecular weights of sialoglycoproteins in these fractions were estimated as 746 kDa (P-Fr. 1-3), 4470 kDa (P-Fr. 1-2) and a more than 10,000 kDa (P-Fr. 1-1), respectively. The other in P-Fr. 2, with molecular weight estimated as 92 kDa, revealed positive but weak reactivity against IV-HA; however, the fraction did not show positive reactivity against either IVs-A or -B. All these fractions contained sialoglycoproteins with a high content of carbohydrates (31.0-59.3%, w/w). From their carbohydrate compositions, it can be suspected that sialoglycoproteins having O-glycosidically linked carbohydrate chains were predominating in the three fractions. In conclusion, it is suggested that at least three sialoglycoproteins with influenza virus-receptor activity showing different chemical properties to each other exist in human meconium.
Ohyama
K
K
Department of Biochemistry, School of Pharmacy, Tokyo University of Pharmacy & Life Science (Formerly Tokyo College of Pharmacy), Japan.
Shinozuka
T
T
Yanagida
J
J
Yamakawa
T
T
D000596:Amino Acids / Q000032:analysis; D002241:Carbohydrates / Q000032:analysis; D002850:Chromatography, Gel; D002851:Chromatography, High Pressure Liquid; D006801:Humans; D008470:Meconium / Q000737:chemistry*; D009975:Orthomyxoviridae / Q000378:metabolism*; D011991:Receptors, Virus / Q000378:metabolism*; D012795:Sialoglycoproteins / Q000737:chemistry / Q000302:isolation & purification* / Q000378:metabolism
D000596:Amino Acids; D002241:Carbohydrates; D011991:Receptors, Virus; D012795:Sialoglycoproteins