Determination of fluoxetine in human plasma using reserved phase HPLC.
A rapid, simple, accurate method for the determination of fluoxetine in human plasma is presented. Liquid-liquid extraction of fluoxetine was carried out using diethyl ether. Chlorprothixene was applied as an internal standard. The samples were chromatographed on a LiChrosorb RP-18 (10 microns) column and the mobile phase was acetonitrile/phosphate buffer pH 2.70 (9:1). The detection was carried at 254 nm. A linear quantitative response curve was generated over a concentration range of 100-600 ng/ml. Overall extraction efficiency of the extraction procedure was found to be 86 to 91% with a correlation coefficient of 0.992.
Misztal
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G
Department of Medicinal Chemistry, Medical Academy, Lublin, Poland.
Hopkała
H
H
D018687:Antidepressive Agents, Second-Generation / Q000097:blood*; D002138:Calibration; D002851:Chromatography, High Pressure Liquid; D005473:Fluoxetine / Q000097:blood*; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007202:Indicators and Reagents
D018687:Antidepressive Agents, Second-Generation; D007202:Indicators and Reagents; D005473:Fluoxetine