Genetic linkage study of a major susceptibility locus (D2S125) in a British population of non-insulin dependent diabetic sib-pairs using a simple non-isotopic screening method.
A polymorphic microsatellite marker (D2S125) was recently reported to show significant linkage to non-insulin dependent diabetes mellitus (NIDDM) in a population of Mexican-American affected sib-pairs. We have used a simple non-isotopic screening technique employing the polymerase chain reaction (PCR) with a biotinylated primer to study the genetic linkage and allele frequency distribution of the D2S125 marker in a population of 109 British NIDDMs (62 possible affected sib-pairs). The analysis provided no evidence for linkage of the D2S125 marker in the British subjects (MLS = 0.029, P > 0.05). The PCR screening method used proved to be a convenient and reliable alternative to the radiolabelling of PCR products.
Thomas
A W
AW
University of Wales College of Medicine, Department of Medicine, Heath Park, Cardiff, UK.
Sherratt
E J
EJ
Gagg
J W
JW
Davies
S A
SA
Majid
A
A
Alcolado
J C
JC
D003924:Diabetes Mellitus, Type 2 / Q000453:epidemiology / Q000235:genetics*; D004198:Disease Susceptibility; D008040:Genetic Linkage; D005820:Genetic Testing / Q000379:methods*; D006801:Humans; D018895:Microsatellite Repeats; D009679:Nuclear Family; D006113:United Kingdom / Q000453:epidemiology