title: Crystallization of the first three domains of the human insulin-like growth factor-1 receptor.
abstract: The insulin-like growth factor-1 receptor (IGF-1R) is a tyrosine kinase receptor of central importance in cell proliferation. A fragment (residues 1-462) comprising the L1-cysteine rich-L2 domains of the human IGF-1R ectodomain has been overexpressed in glycosylation-deficient Lec8 cells and has been affinity-purified via a c-myc tag followed by gel filtration. The fragment was recognized by two anti-IGF-1R monoclonal antibodies, 24-31 and 24-60, but showed no detectable binding of IGF-1 or IGF-2. Isocratic elution of IGF-1R/462 on anion-exchange chromatography reduced sample heterogeneity, permitting the production of crystals that diffracted to 2.6 A resolution with cell dimensions a = 77.0 A, b = 99.5 A, c = 120.1 A, and space group P2(1)2(1)2(1).
authors.affiliation: CSIRO Division of Molecular Science, Parkville, Victoria, Australia.
authors.forename: N M
authors.initials: NM
authors.lastname: McKern
authors.forename: M
authors.initials: M
authors.lastname: Lou
authors.forename: M J
authors.initials: MJ
authors.lastname: Frenkel
authors.forename: A
authors.initials: A
authors.lastname: Verkuylen
authors.forename: J D
authors.initials: JD
authors.lastname: Bentley
authors.forename: G O
authors.initials: GO
authors.lastname: Lovrecz
authors.forename: N
authors.initials: N
authors.lastname: Ivancic
authors.forename: T C
authors.initials: TC
authors.lastname: Elleman
authors.forename: T P
authors.initials: TP
authors.lastname: Garrett
authors.forename: L J
authors.initials: LJ
authors.lastname: Cosgrove
authors.forename: C W
authors.initials: CW
authors.lastname: Ward
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D016466:CHO Cells; D002850:Chromatography, Gel; D002852:Chromatography, Ion Exchange; D006224:Cricetinae; D003460:Crystallization; D018360:Crystallography, X-Ray; D006801:Humans; D008969:Molecular Sequence Data; D010446:Peptide Fragments / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification; D017526:Receptor, IGF Type 1 / Q000737:chemistry* / Q000235:genetics; D011994:Recombinant Proteins; D014162:Transfection
chemical_list: D010446:Peptide Fragments; D011994:Recombinant Proteins; D017526:Receptor, IGF Type 1