title: Genetic analysis of plasmid-specific pheromone signaling encoded by pPD1 in Enterococcus faecalis.
abstract: Certain plasmids in Enterococcus faecalis encode a mating response to recipient-produced peptide sex pheromones. Targeted disruption of tra genes on pPD1 suggested that TraA plays a central role in the plasmid-specific pheromone signaling pathway. TraA functioned as a negative regulator for the pheromone-inducible conjugal transfer. Complementation analysis of pPD1 tra gene mutants by pAD1 suggested that the pheromone binding function of TraC was non-specific between these plasmids, but the function of TraA and the pheromone shutdown function of TraB are plasmid-specific.
authors.affiliation: Department of Applied Biological Chemistry, University of Tokyo, Japan.
authors.forename: J
authors.initials: J
authors.lastname: Nakayama
authors.forename: A
authors.initials: A
authors.lastname: Suzuki
mesh_terms: D001430:Bacteriocins / Q000235:genetics*; D003227:Conjugation, Genetic / Q000235:genetics / Q000502:physiology; D013293:Enterococcus faecalis / Q000235:genetics* / Q000502:physiology*; D005798:Genes, Bacterial; D005816:Genetic Complementation Test; D010957:Plasmids / Q000235:genetics*; D012680:Sensitivity and Specificity; D012724:Sex Attractants / Q000235:genetics* / Q000502:physiology*; D015398:Signal Transduction / Q000502:physiology
chemical_list: D001430:Bacteriocins; D012724:Sex Attractants