Positive selection of CD56+ lymphocytes by magnetic cell sorting.
A new method for the isolation of CD56+ lymphocytes from peripheral mononuclear blood cells is described. Magnetic microbeads conjugated to goat antimouse antiserum in combination with a murine monoclonal anti-CD56 antibody were coated to the CD56+ target cells. CD56+ cells were then isolated with the use of a magnetic cell sorter. The purity of the CD56+ cells was 98.4 +/- 1% (n = 12) with a recovery of the CD56+ lymphocytes of 57.2 +/- 9% (range 48-77%). The natural killer, activity of the CD56+ lymphocytes as well as the interleukin-2-induced proliferative response were not affected by the isolation procedure or the presence of the magnetic microbeads on the CD56+ cells. The described method might be a useful tool for the further characterization of CD56+ cells and their subsets and can easily be upgraded for clinical use in adoptive immunotherapy with CD56+ lymphocytes.
Geiselhart
A
A
University Children's Hospital, Department of Hematology/Oncology, Tübingen, Germany.
Neu
S
S
Buchholz
F
F
Lang
P
P
Niethammer
D
D
Handgretinger
R
R
D000328:Adult; D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D019002:CD56 Antigen / Q000032:analysis* / Q000276:immunology; D002455:Cell Division; D003602:Cytotoxicity, Immunologic; D006041:Goats; D006801:Humans; D018189:Immunomagnetic Separation / Q000379:methods*; D008213:Lymphocyte Activation; D016131:Lymphocyte Subsets / Q000737:chemistry*; D051379:Mice
D000911:Antibodies, Monoclonal; D019002:CD56 Antigen