title: Detection and characterization of weak affinity antibody antigen recognition with biomolecular interaction analysis.
abstract: In biological systems, weak-affinity interactions (association constant, Ka, of less than approximately 10(4) M-1) between biomolecules are common and essential to the integrity of such units. However, studies of weak biological interactions are difficult due to the scarcity of analytical methods available for the bioscientist. In this communication, we report on the use of biosensors based on surface plasmon resonance to detect and characterize weak affinity antibody-antigen interactions. Monoclonal antibodies towards carbohydrate antigens were immobilized on sensor surfaces and were used to detect weak binding of the carbohydrate tetraglucose of dissociation constant, Kd, in the millimolar range. Sensorgrams were received in the form of square pulses where the kinetic rate constants were difficult to assess due to the rapid association and dissociation of the antigen to/from the immobilized antibody.
authors.affiliation: Department of Natural Sciences, University of Kalmar, Sweden.
authors.forename: S
authors.initials: S
authors.lastname: Ohlson
authors.forename: M
authors.initials: M
authors.lastname: Strandh
authors.forename: H
authors.initials: H
authors.lastname: Nilshans
mesh_terms: D000911:Antibodies, Monoclonal / Q000276:immunology / Q000378:metabolism; D000937:Antigen-Antibody Reactions; D015374:Biosensing Techniques; D002240:Carbohydrate Sequence; D008969:Molecular Sequence Data; D009844:Oligosaccharides / Q000276:immunology / Q000378:metabolism; D011485:Protein Binding; D012031:Refractometry
chemical_list: D000911:Antibodies, Monoclonal; D009844:Oligosaccharides