Tumor site-selective localization of an adoptively transferred T cell line expressing a macrophage lectin.
CTLL-2 cells were transfected with an expression vector that contained cDNA of a mouse macrophage galactose/N-acetylgalactosamine-specific calcium-type lectin (MMGL) and a stable transfectant (CTL-ML) was established. These cells and mock transfectant cells (CTL-CEP) were labeled with a long-term fluorescent cell tracer, DiI. The labeled cells were intravenously injected into mice that contained established lung metastases produced by OV2944-HM-1 ovarian tumor cells. Analyses with fluorescence microscopy of a series of frozen lung sections from the recipient mice revealed that CTL-ML preferentially accumulated in the lung metastatic nodules, whereas CTL-CEP did not. Time course studies showed that the preferential accumulation was evident 3 days after adoptive transfer. We also found that OV2944-HM-1 cells bound peanut agglutinin and Vicia villosa agglutinin B4, whose sugar specificity overlaps with the specificity of MMGL. These results suggested that MMGL molecules expressed on CTLL-2 cells contributed to their selective trafficking or retention in tumor foci possibly through recognition of tumor-associated cell surface carbohydrate antigens. These results also suggested that MMGL could be used for the selective targeting of effector cells in adoptive immunotherapy.
Ichii
S
S
Department of Cancer Biology and Molecular Immunology, Faculty of Pharmaceutical Sciences, The University of Tokyo, Japan.
Imai
Y
Y
Irimura
T
T
D019264:Adoptive Transfer; D000818:Animals; D001212:Asialoglycoproteins; D002352:Carrier Proteins / Q000276:immunology*; D002455:Cell Division; D002465:Cell Movement / Q000276:immunology*; D005260:Female; D037102:Lectins / Q000276:immunology*; D037181:Lectins, C-Type; D008175:Lung Neoplasms / Q000276:immunology / Q000556:secondary / Q000628:therapy; D008565:Membrane Proteins; D051379:Mice; D009374:Neoplasms, Experimental / Q000276:immunology* / Q000473:pathology* / Q000628:therapy; D010051:Ovarian Neoplasms / Q000276:immunology* / Q000473:pathology* / Q000628:therapy; D013601:T-Lymphocytes / Q000276:immunology / Q000473:pathology*
D001212:Asialoglycoproteins; D002352:Carrier Proteins; C064720:Clec10a protein, mouse; D037102:Lectins; D037181:Lectins, C-Type; D008565:Membrane Proteins