Effect of pentoxifylline on motility and longevity of fresh and thawed dog spermatozoa.
The effect of pentoxifylline on fresh and frozen-thawed dog spermatozoa was evaluated. In Expt 1, 0.0036 mol l-1 or 0.0072 mol l-1 pentoxifylline was added to fresh dog spermatozoa. Semen was incubated at 25 degrees C or 39 degrees C. In Expt 2, pentoxifylline was added either before freezing or in the process of thawing at a final concentration of 0.0072 mol l-1. Addition of pentoxifylline significantly increased the percentage of progressively motile spermatozoa in fresh semen. The most beneficial effect of pentoxifylline on frozen-thawed semen was observed (P < 0.001) when it was added to semen in the process of thawing.
Koutsarova
N
N
Department of Reproduction, Thracian University, Stara Zagora, Bulgaria.
Todorov
P
P
Koutsarov
G
G
D000818:Animals; D002470:Cell Survival / Q000187:drug effects; D015925:Cryopreservation / Q000662:veterinary*; D004285:Dogs; D008297:Male; D010431:Pentoxifylline / Q000494:pharmacology*; D010726:Phosphodiesterase Inhibitors / Q000494:pharmacology*; D012662:Semen Preservation / Q000662:veterinary*; D013081:Sperm Motility / Q000187:drug effects*; D013094:Spermatozoa / Q000187:drug effects* / Q000502:physiology
D010726:Phosphodiesterase Inhibitors; D010431:Pentoxifylline