Fast isolation of RNA to detect expression of tumor markers.
The expression status of several tumor-related proteins is of great interest in clinical examination and research. As a completion to conventional antibody staining, RT-PCR is often used today. Reliable isolation of RNA from a low number of cells is very often a critical stage of such an examination. We demonstrate here a simple and fast method to isolate RNA from only 10,000 cells and applied it to the detection of CEA, c-ERB-B2, and mdr-1 as often studied models for tumor markers.
Schenk
J A
JA
Max Delbrück Center for Molecular Medicine (MDC) Berlin-Buch, Berlin, Germany.
Hillebrand
T
T
Lübbe
L
L
Heymann
S
S
Böttger
M
M
Micheel
B
B
Bendzko
P
P
D020168:ATP Binding Cassette Transporter, Subfamily B, Member 1 / Q000032:analysis / Q000235:genetics; D014408:Biomarkers, Tumor / Q000032:analysis*; D002272:Carcinoembryonic Antigen / Q000032:analysis / Q000235:genetics; D015870:Gene Expression; D006801:Humans; D012334:RNA, Neoplasm / Q000302:isolation & purification*; D012194:RNA-Directed DNA Polymerase; D018719:Erb-b2 Receptor Tyrosine Kinases / Q000032:analysis / Q000235:genetics; D014407:Tumor Cells, Cultured
D020168:ATP Binding Cassette Transporter, Subfamily B, Member 1; D014408:Biomarkers, Tumor; D002272:Carcinoembryonic Antigen; D012334:RNA, Neoplasm; D012194:RNA-Directed DNA Polymerase; D018719:Erb-b2 Receptor Tyrosine Kinases