Disinfection of cell-associated and extracellular HIV-1 by PUVA treatment.
To inactivate cell-associated and extracellular HIV-1 while preserving cellular surface antigens, a procedure was used based on PUVA treatment, i.e. addition of psoralen to cell suspensions followed by irradiation with UVA light. T-lymphoid MT-4 cells were infected with HIV-1 strain NL4-3, 4'-aminomethyl-4,5',8-trimethylpsoralen was added, and the cell suspension was irradiated with 20 mW/cm2 UVA light for 3, 4 and 5 min. To evaluate virus inactivation, cells and supernatants were diluted serially and cocultured with uninfected MT-4 cells. Infectious HIV-1 was detected by cytopathic effects, immunofluorescence and p24 antigen ELISA. UVA irradiation at 3.6 J/cm2 (3 min 20 mW/cm2) reduced the amounts of both cell-associated and extracellular infectious HIV-1 by more than five orders of magnitude. Even at more stringent conditions of PUVA treatment (10 min 20 mW/cm2 UVA irradiation), conformational cellular surface epitopes remained detectable by flow cytometry.
Deichmann
M
M
Deutsches Krebsforschungszentrum, Heidelberg, Germany.
Sczakiel
G
G
Haas
R
R
D019380:Anti-HIV Agents / Q000494:pharmacology*; D002461:Cell Line, Transformed; D003588:Cytopathogenic Effect, Viral; D004797:Enzyme-Linked Immunosorbent Assay; D005434:Flow Cytometry; D019084:Fluorescent Antibody Technique, Indirect; D016655:HIV Core Protein p24 / Q000032:analysis; D015497:HIV-1 / Q000187:drug effects* / Q000378:metabolism / Q000502:physiology / Q000528:radiation effects; D006801:Humans; D017319:Photosensitizing Agents / Q000494:pharmacology*; D014307:Trioxsalen / Q000031:analogs & derivatives* / Q000494:pharmacology; D014466:Ultraviolet Rays
D019380:Anti-HIV Agents; D016655:HIV Core Protein p24; D017319:Photosensitizing Agents; C018822:aminomethyltrioxsalen; D014307:Trioxsalen