title: Effects of sleep on wake-induced c-fos expression.
abstract: We investigated the effects of sleep on wake-induced c-fos expression in the cerebral cortex of rats and c-fos-lacZ transgenic mice. In the cortex of rats, the levels of c-Fos, detected both by immunocytochemistry and Western blot, remained high during 6 or 12 hr of enforced wakefulness but declined rapidly (within 1 hr) with increasing time of recovery sleep. Similarly, in the transgenic mice in which lacZ expression is driven from the c-fos promoter, beta-galactosidase activity was high after enforced wakefulness and declined with increasing amounts of sleep. These results suggest that the decrease in c-Fos protein in cortical neurons during sleep may be attributable to cessation of c-fos expression, activation of a process that degrades the wake-induced c-Fos, or both.
authors.affiliation: VA Medical Center and Harvard Medical School, Brockton, Massachusetts 02401, USA.
authors.forename: R
authors.initials: R
authors.lastname: Basheer
authors.forename: J E
authors.initials: JE
authors.lastname: Sherin
authors.forename: C B
authors.initials: CB
authors.lastname: Saper
authors.forename: J I
authors.initials: JI
authors.lastname: Morgan
authors.forename: R W
authors.initials: RW
authors.lastname: McCarley
authors.forename: P J
authors.initials: PJ
authors.lastname: Shiromani
mesh_terms: D000818:Animals; D001143:Arousal / Q000502:physiology*; D015153:Blotting, Western; D015870:Gene Expression / Q000502:physiology; D017930:Genes, Reporter; D006179:Gyrus Cinguli / Q000737:chemistry / Q000502:physiology; D007150:Immunohistochemistry; D007763:Lac Operon / Q000502:physiology; D008297:Male; D051379:Mice; D008822:Mice, Transgenic; D016760:Proto-Oncogene Proteins c-fos / Q000032:analysis / Q000235:genetics* / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley; D012890:Sleep / Q000502:physiology*; D013045:Species Specificity; D019076:Transgenes / Q000502:physiology; D001616:beta-Galactosidase / Q000235:genetics
chemical_list: D016760:Proto-Oncogene Proteins c-fos; D001616:beta-Galactosidase