Different expression of adhesion molecules on myeloid and B-lymphoid CD34+ progenitors in normal bone marrow.
The expression of adhesion molecules was studied on B lymphoid and myeloid CD34+ precursors in normal bone marrow. Bone marrow aspirates were labelled in a double fluorescence procedure with the CD34 monoclonal antibody 43A1 and with antibodies directed against maturation and differentiation antigens and adhesion molecules. Three clusters of CD34+ cells could be distinguished by their light scatter characteristics in flow cytometry. The population with the lowest forward scatter contained B-lymphoid precursors while the two others showed phenotypic characteristics of, respectively, early and late myeloid precursors. Nearly all CD34+ cells in the 3 subpopulations expressed VLA-4, VLA-5, LFA-3 and H-CAM. B-lymphoid progenitors showed a higher density of VLA-4 and VLA-5 than the myeloid progenitors. Myeloid precursors, and particularly the late subset, expressed more HCAM than the B-lymphoid progenitors. The majority of the CD34+ cells also expressed LFA-1 and L-selectin. Higher numbers of positive cells were found in the myeloid subset. The early myeloid subset showed the highest positivity for L-selectin. We conclude that B lymphoid and early and late myeloid CD34+ precursors in normal bone marrow show a different profile of adhesion molecules. These profiles could reflect a higher tendency of the myeloid CD34+ precursors to circulate.
De Waele
M
M
Department of Laboratory Haematology, Academic Hospital, Free University Brussels (VUB).
Renmans
W
W
Damiaens
S
S
Flament
J
J
Schots
R
R
Van Riet
I
I
D000328:Adult; D015703:Antigens, CD / Q000032:analysis; D018952:Antigens, CD34 / Q000032:analysis*; D001402:B-Lymphocytes / Q000737:chemistry* / Q000276:immunology; D001854:Bone Marrow Cells / Q000737:chemistry* / Q000276:immunology; D018968:CD58 Antigens / Q000032:analysis; D015815:Cell Adhesion Molecules / Q000032:analysis*; D006098:Granulocytes / Q000737:chemistry* / Q000276:immunology; D006412:Hematopoietic Stem Cells / Q000737:chemistry* / Q000276:immunology; D006801:Humans; D018960:Hyaluronan Receptors / Q000032:analysis; D039041:Integrin alpha4beta1; D016023:Integrins / Q000032:analysis; D019041:L-Selectin / Q000032:analysis; D017469:Receptors, Fibronectin / Q000032:analysis; D016201:Receptors, Lymphocyte Homing / Q000032:analysis
D015703:Antigens, CD; D018952:Antigens, CD34; D018968:CD58 Antigens; D015815:Cell Adhesion Molecules; D018960:Hyaluronan Receptors; D039041:Integrin alpha4beta1; D016023:Integrins; D017469:Receptors, Fibronectin; D016201:Receptors, Lymphocyte Homing; D019041:L-Selectin