Ia expression and antigen presentation by glia: strain and cell type-specific differences among rat astrocytes and microglia.
Astrocytes from experimental allergic encephalomyelitis (EAE)-susceptible Lewis rats expressed higher levels of Interferon-gamma-inducible Ia than astrocytes from EAE-resistant Brown Norway (BN) rats, whereas BN microglia expressed higher Ia than Lewis at both mRNA and protein levels. Lewis astrocytes induced proliferation of MBP-specific T cells selected on Lewis background as efficiently as Lewis thymocytes, whereas BN astrocytes were much less efficient in stimulating T cells selected in the presence of BN thymocytes. Microglia, irrespective of strain, induced only weak proliferative responses of these T cells despite the high expression of Ia. Antigen-stimulated T cells underwent apoptosis in the presence of microglia but not astrocytes. Thus, astrocyte-mediated proliferation of MBP-specific T cells may contribute to the development of EAE, while microglia-induced T cell apoptosis may downregulate immunopathological processes in the brain.
Klyushnenkova
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EN
University of Maryland at Baltimore, School of Medicine, Department of Neurology 21201, USA.
Vanguri
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D000818:Animals; D000938:Antigen-Presenting Cells / Q000276:immunology*; D000941:Antigens / Q000276:immunology; D000954:Antigens, Surface / Q000276:immunology; D017209:Apoptosis / Q000502:physiology; D001253:Astrocytes / Q000276:immunology* / Q000502:physiology; D002455:Cell Division / Q000502:physiology; D002478:Cells, Cultured; D005786:Gene Expression Regulation / Q000502:physiology; D000949:Histocompatibility Antigens Class II / Q000235:genetics / Q000276:immunology*; D007371:Interferon-gamma / Q000494:pharmacology; D017628:Microglia / Q000276:immunology* / Q000502:physiology; D004676:Myelin Basic Protein / Q000276:immunology; D051381:Rats; D011914:Rats, Inbred BN; D011917:Rats, Inbred Lew; D013045:Species Specificity; D013601:T-Lymphocytes / Q000166:cytology / Q000276:immunology / Q000502:physiology
D000941:Antigens; D000954:Antigens, Surface; D000949:Histocompatibility Antigens Class II; D004676:Myelin Basic Protein; D007371:Interferon-gamma