An efficient DNA extraction method for small metazoans.
The isolation of total nucleic acids from small metazoan taxa is difficult and often leads to an unacceptably large percentage of unsuccessful polymerase chain reaction (PCR) amplifications. Our work with the evolutionary genetics of harpacticoid copepods was an incentive to refine techniques such that consistent amplifications from minute marine organisms were feasible. We describe these modifications and demonstrate their utility for the amplification of multiple loci from single harpacticoid copepods.
Schizas
N V
NV
Department of Biological Sciences, University of South Carolina, Columbia 29208, USA.
Street
G T
GT
Coull
B C
BC
Chandler
G T
GT
Quattro
J M
JM
D000818:Animals; D003445:Crustacea / Q000235:genetics*; D004247:DNA / Q000302:isolation & purification*; D003576:Electron Transport Complex IV / Q000235:genetics; D005821:Genetic Techniques; D016133:Polymerase Chain Reaction / Q000379:methods
D004247:DNA; D003576:Electron Transport Complex IV