title: An effective method of completely removing contaminating genomic DNA from an RNA sample to be used for PCR.
abstract: A simple method for removing contaminating genomic DNA from an RNA preparation is presented. The method involves digestion of the RNA with RNase-free DNase I at room temperature followed by inactivation of the enzyme at 65 degrees C in presence of EDTA. This method produces an RNA sample that is negative for genomic DNA by PCR.
authors.affiliation: Department of Orthopedics, Mayo Clinic, Rochester, MN 55905, USA.
authors.forename: A
authors.initials: A
authors.lastname: Sanyal
authors.forename: S W
authors.initials: SW
authors.lastname: O'Driscoll
authors.forename: M E
authors.initials: ME
authors.lastname: Bolander
authors.forename: G
authors.initials: G
authors.lastname: Sarkar
mesh_terms: D001671:Biochemistry / Q000379:methods*; D004247:DNA / Q000737:chemistry*; D018076:DNA, Complementary; D003850:Deoxyribonuclease I / Q000737:chemistry; D004492:Edetic Acid / Q000737:chemistry; D016133:Polymerase Chain Reaction / Q000379:methods*; D012313:RNA / Q000302:isolation & purification*
chemical_list: D018076:DNA, Complementary; D012313:RNA; D004247:DNA; D004492:Edetic Acid; D003850:Deoxyribonuclease I