Stereospecific determination of mefloquine in biological fluids by high-performance liquid chromatography.
A sensitive stereoselective HPLC method was developed for determination of mefloquine (MFQ) enantiomers in plasma, urine and whole blood. The assay involved liquid-liquid extraction of MFQ from biological fluids with a mixture of hexane and isopropanol in the presence of sodium hydroxide and derivatization of the residue by (+)-(S)-naphthylethylisocyanate (NEIC) as chiral derivatizing reagent. Separation of the resulting diastereomers was performed on a silica normal-phase column using chloroform-hexane-methanol (25:74:1) as the mobile phase with a flow-rate of 1 ml/min. Using 200 microl of plasma or whole blood, the limit of determination was 0.2 microg/ml with UV detection for both enantiomers. The limit of determination in 500 microl of urine was 0.08 microg/ml with UV detection.
Souri
E
E
Department of Medicinal Chemistry, Faculty of Pharmacy, University of Medical Sciences, Tehran, Iran.
Farsam
H
H
Jamali
F
F
D000818:Animals; D000962:Antimalarials / Q000097:blood* / Q000737:chemistry / Q000652:urine*; D002851:Chromatography, High Pressure Liquid; D008297:Male; D015767:Mefloquine / Q000097:blood* / Q000737:chemistry / Q000652:urine*; D051381:Rats; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013056:Spectrophotometry, Ultraviolet; D013237:Stereoisomerism
D000962:Antimalarials; D015767:Mefloquine