title: Construction of a 780-kb PAC, BAC, and cosmid contig encompassing the minimal critical deletion involved in B cell chronic lymphocytic leukemia at 13q14.3.
abstract: A putative tumor suppressor gene involved in B cell chronic lymphocytic leukemia (B-CLL) was mapped to human chromosome 13q14.3 close to the genetic markers D13S25 and D13S319. We constructed a 780-kb-long contig composed of cosmids, bacterial artificial chromosomes, and bacteriophage P1-derived artificial chromosomes that provides essential information and tools for the positional cloning of this gene. The conting contains both flanking markers as well as several additional genetic markers, three ESTs, and one potential CpG island. In addition, using one B-CLL patient, we characterized a small internal deleted region of 550 kb. Comparing this deletion with other recently published deletions narrows the minimally deleted area to less than 100 kb in our physical map. This deletion core region should contain all or part of the disrupted in B cell malignancies tumor suppressor gene.
authors.affiliation: Institut de Biologie de l'Hôtel-Dieu, INSERM U 463, Centre Hospitalier Universitaire, Nantes, France.
authors.forename: I
authors.initials: I
authors.lastname: Bouyge-Moreau
authors.forename: G
authors.initials: G
authors.lastname: Rondeau
authors.forename: H
authors.initials: H
authors.lastname: Avet-Loiseau
authors.forename: M T
authors.initials: MT
authors.lastname: André
authors.forename: S
authors.initials: S
authors.lastname: Bézieau
authors.forename: M
authors.initials: M
authors.lastname: Chérel
authors.forename: S
authors.initials: S
authors.lastname: Saleün
authors.forename: E
authors.initials: E
authors.lastname: Cadoret
authors.forename: T
authors.initials: T
authors.lastname: Shaikh
authors.forename: M M
authors.initials: MM
authors.lastname: De Angelis
authors.forename: S
authors.initials: S
authors.lastname: Arcot
authors.forename: M
authors.initials: M
authors.lastname: Batzer
authors.forename: J P
authors.initials: JP
authors.lastname: Moisan
authors.forename: M C
authors.initials: MC
authors.lastname: Devilder
mesh_terms: D001435:Bacteriophages / Q000235:genetics; D018244:Chromosomes, Artificial, Yeast; D002876:Chromosomes, Bacterial; D002882:Chromosomes, Human, Pair 13; D003360:Cosmids / Q000235:genetics*; D005819:Genetic Markers; D006579:Heterozygote; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D015451:Leukemia, Lymphocytic, Chronic, B-Cell / Q000235:genetics*; D017384:Sequence Deletion; D016324:Sequence Tagged Sites
chemical_list: D005819:Genetic Markers