Plant regeneration of indica rice (Oryza sativa) cultivars from mature embryo-derived calli.
Plant regeneration from seven-week-old callus cultures derived from mature embryos of several indica rice cultivars was achieved with frequencies of morphogenic calli from 10 to 47%. Three media were tested both for callogenesis and plant regeneration. For 3 of the 7 genotypes examined, the best combination of media for plant regeneration was Murashige & Skoog basal medium: MSC (callogenesis) and MSR (regeneration). The rates of callogenesis were not related to the capacity for plant regeneration. Two genotypes CR-1113 and CR-5272 produced the highest number of regenerated green plants. The results of this study suggest that genetic differences could be directly linked to the ability to regenerate in these plant cultivars.
Valdez
M
M
Escuela de Biología, Universidad de Costa Rica, San José, Costa Rica.
Muñoz
M
M
Vega
J R
JR
Espinoza
A M
AM
D046508:Culture Techniques / Q000191:economics; D048091:Guided Tissue Regeneration / Q000379:methods*; D012275:Oryza / Q000196:embryology / Q000235:genetics* / Q000254:growth & development* / Q000821:virology