Cholesterol derivative of poly(ethylene glycol) inhibits clathrin-independent, but not clathrin-dependent endocytosis.
The effect of poly(ethylene glycol) cholesteryl ethers (PEG(n)-Chols) with two different numbers of units (n = 50 and 200) in the hydrophilic PEG moiety on cellular endocytic activity was studied on HT-1080 cells. The amphipathic molecules were soluble in aqueous solution. When fluorescein derivatives of PEG-Chols (one fluorescein at the distal end of PEG) were incubated with the cells in culture, the cellular fluorescence was localized at the plasma membrane level and in intracellular vesicles. Fluorescence quantification indicated that for the same external concentration, twice more FPEG(50)-Chol than FPEG(200)-Chol was associated with the cells under the same conditions. Regardless of the length of PEG moiety, PEG-Chols' interaction with cells reduced the endocytic internalization of a fluid phase marker, horseradish peroxidase (HRP) depending on the cell-associated amount. In contrast, internalization of 125I-labeled epidermal growth factor (EGF) through receptor-mediated endocytosis did not change upon incubation with PEG(50)-Chol. The effect of PEG(200)-Chol was also small, since EGF internalization showed a reduction of 10-20%, while at the same concentration as much as 80% of HRP uptake was inhibited. PEG(50)-Chol did not influence the internalization of a larger ligand, 125I-transferrin (Tfn). However, in the presence of PEG(200)-Chol, the uptake of 125I-Tfn decreased remarkably, and yet, PEG(200)-Chol has no influence on the binding and internalization of a monoclonal antibody directed toward the ectodomain of the Tfn-receptor. These results suggested that incorporation of PEG-Chols in the outer monolayer of the plasma membrane specifically inhibited clathrin-independent, but not clathrin-dependent endocytosis.
Ishiwata
H
H
Department of Physical Chemistry, Faculty of Pharmaceutical Sciences, Kyoto University, Japan.
Sato
S B
SB
Vertut-Doï
A
A
Hamashima
Y
Y
Miyajima
K
K
D001798:Blood Proteins / Q000494:pharmacology; D002460:Cell Line; D002462:Cell Membrane / Q000187:drug effects; D002784:Cholesterol / Q000031:analogs & derivatives* / Q000737:chemistry / Q000378:metabolism / Q000494:pharmacology; D002966:Clathrin / Q000494:pharmacology*; D004705:Endocytosis / Q000187:drug effects*; D004815:Epidermal Growth Factor / Q000378:metabolism; D004987:Ethers / Q000494:pharmacology; D005452:Fluoresceins / Q000378:metabolism; D006735:Horseradish Peroxidase / Q000378:metabolism; D006801:Humans; D007700:Kinetics; D008074:Lipoproteins / Q000494:pharmacology; D018613:Microscopy, Confocal; D015394:Molecular Structure; D011092:Polyethylene Glycols / Q000737:chemistry / Q000378:metabolism / Q000494:pharmacology*; D011990:Receptors, Transferrin / Q000378:metabolism; D012235:Rhodamines / Q000378:metabolism; D013696:Temperature; D014168:Transferrin / Q000378:metabolism
D001798:Blood Proteins; D002966:Clathrin; D004987:Ethers; D005452:Fluoresceins; D008074:Lipoproteins; D011990:Receptors, Transferrin; D012235:Rhodamines; D014168:Transferrin; C095164:poly(oxyethylene) cholesteryl ether; C022027:lissamine rhodamine B; D011092:Polyethylene Glycols; D004815:Epidermal Growth Factor; D002784:Cholesterol; D006735:Horseradish Peroxidase