title: Cloning of two novel human importin-alpha subunits and analysis of the expression pattern of the importin-alpha protein family.
abstract: The import of many proteins into the nucleus is mediated by the importin-alpha/beta heterodimer. While only one importin-beta gene has been found, several forms of importin-alpha have been described. In addition to the three human importin-alphas already identified, we report here the primary structure of two new human importin-alpha proteins. The five known human importin-alpha subunits can be classified into three subfamilies that appear conserved in higher eukaryotic organisms. We show by immunoblotting that the different importin-alpha subfamilies are expressed in a variety of human tissues and mammalian cell lines.
authors.affiliation: Franz Volhard Clinic, Humboldt University of Berlin, Germany.
authors.forename: M
authors.initials: M
authors.lastname: Köhler
authors.forename: S
authors.initials: S
authors.lastname: Ansieau
authors.forename: S
authors.initials: S
authors.lastname: Prehn
authors.forename: A
authors.initials: A
authors.lastname: Leutz
authors.forename: H
authors.initials: H
authors.lastname: Haller
authors.forename: E
authors.initials: E
authors.lastname: Hartmann
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D018076:DNA, Complementary; D015870:Gene Expression; D006367:HeLa Cells; D006801:Humans; D015151:Immunoblotting; D019169:Jurkat Cells; D028884:Karyopherins; D008969:Molecular Sequence Data; D009687:Nuclear Proteins / Q000096:biosynthesis* / Q000145:classification / Q000235:genetics / Q000276:immunology; D012333:RNA, Messenger; D011817:Rabbits; D017386:Sequence Homology, Amino Acid; D014018:Tissue Distribution; D014407:Tumor Cells, Cultured
chemical_list: D018076:DNA, Complementary; D028884:Karyopherins; D009687:Nuclear Proteins; D012333:RNA, Messenger