title: Diarrhoeal toxin production at low temperature by selected strains of Bacillus cereus.
abstract: The growth of four Bacillus cereus strains producing diarrhoeal toxin at 32 degrees C (F4433/73 and 29.155, isolated on the occasion of foodborne outbreaks, and F4581/76L and F4581/76R, two variants of a clinical strain), a weakly toxigenic strain isolated in routine analysis of food (3505M) and an emetic isolate (F3502/73) was investigated at low temperature. Biomass was determined by protein assay. Generation times were: for strain F3502/73, which grew at > or = 12 degrees C, 8.71 h (at 12 degrees C); for other strains, which grew at > or = 10 degrees C, 10.2 to approximately 18.9 h (at 10 degrees C). Toxin production during growth was evaluated by a commercial kit (Oxoid) and by a toxicity test on Chinese hamster ovary cells. Strains F4433/73 and F4581/76, secreting high levels of diarrhoeal toxin during the exponential phase at 32 degrees C, produced high levels of toxicity at 10 degrees C until the stationary phase. Strain 29.155 had decreased toxin production at 10 degrees C. Toxicities for cellular extracts remained low when compared with culture filtrates. A correlation was found between the toxicity values given by the two detection methods tested, and the suitability of both methods for the detection of potential poisoning isolates is discussed.
authors.affiliation: Laboratoire Central d'Hygiène Alimentaire, Centre National d'Etudes Vétérinaires et Alimentaires, Maison-Alfort, France.
authors.forename: C
authors.initials: C
authors.lastname: Fermanian
authors.forename: C
authors.initials: C
authors.lastname: Lapeyre
authors.forename: J M
authors.initials: JM
authors.lastname: Fremy
authors.forename: M
authors.initials: M
authors.lastname: Claisse
mesh_terms: D000818:Animals; D001409:Bacillus cereus / Q000254:growth & development / Q000378:metabolism*; D001427:Bacterial Toxins / Q000096:biosynthesis* / Q000633:toxicity; D016466:CHO Cells; D003080:Cold Temperature; D006224:Cricetinae; D017077:Culture Media, Conditioned; D003967:Diarrhea / Q000382:microbiology*; D008892:Milk / Q000382:microbiology
chemical_list: D001427:Bacterial Toxins; D017077:Culture Media, Conditioned