Development of an epithelium-specific expression cassette with human DNA regulatory elements for transgene expression in lung airways.
The efficient expression of therapeutic genes in target cells or tissues is an important component of efficient and safe gene therapy. Utilizing regulatory elements from the human cytokeratin 18 (K18) gene, including 5' genomic sequences and one of its introns, we have developed a novel expression cassette that can efficiently express reporter genes, as well as the human cystic fibrosis transmembrane conductance regulator (CFTR) gene, in cultured lung epithelial cells. CFTR transcripts expressed from the native K18 enhancer/promoter include two alternative splicing products, due to the activation of two cryptic splice sites in the CFTR coding region. Modification of the K18 intron and CFTR cDNA sequences eliminated the cryptic splice sites without changing the CFTR amino acid sequence, and led to enhanced CFTR mRNA and protein expression as well as biological function. Transgenic expression analysis in mice showed that the modified expression cassette can direct efficient and epithelium-specific expression of the Escherichia coli LacZ gene in the airways of fetal lungs, with no detectable expression in lung fibroblasts or endothelial cells. This is the first expression cassette which selectively directs lung transgene expression for CFTR gene therapy to airway epithelia.
Chow
Y H
YH
Division of Respiratory Research and Lung Gene Therapy Programme, University of Toronto, Toronto, ON, M5G 1X8 Canada.
O'Brodovich
H
H
Plumb
J
J
Wen
Y
Y
Sohn
K J
KJ
Lu
Z
Z
Zhang
F
F
Lukacs
G L
GL
Tanswell
A K
AK
Hui
C C
CC
Buchwald
M
M
Hu
J
J
D000818:Animals; D019556:COS Cells; D002478:Cells, Cultured; D019005:Cystic Fibrosis Transmembrane Conductance Regulator / Q000235:genetics*; D004247:DNA / Q000235:genetics; D004742:Enhancer Elements, Genetic; D004848:Epithelium / Q000502:physiology; D005786:Gene Expression Regulation; D018014:Gene Transfer Techniques; D017930:Genes, Reporter; D015316:Genetic Therapy; D006801:Humans; D007633:Keratins / Q000235:genetics; D008168:Lung / Q000502:physiology*; D051379:Mice; D008822:Mice, Transgenic; D011401:Promoter Regions, Genetic
C505032:CFTR protein, human; D019005:Cystic Fibrosis Transmembrane Conductance Regulator; D007633:Keratins; D004247:DNA