High-level overexpression of yeast elongation factor 3 and detailed kinetic analysis using a coupled spectrophotometric assay.
Sarthy
A V
AV
Pharmaceutical Products Division, Abbott Laboratories, Abbott Park, Illinois 60064-3500, USA.
McGonigal
T
T
Capobianco
J O
JO
Holzman
T H
TH
Walter
K A
KA
Egan
D A
DA
Goldman
R C
RC
D000255:Adenosine Triphosphate / Q000378:metabolism; D000426:Alcohol Dehydrogenase / Q000235:genetics; D015153:Blotting, Western; D003001:Cloning, Molecular; D005656:Fungal Proteins; D007700:Kinetics; D010445:Peptide Elongation Factors / Q000235:genetics / Q000378:metabolism*; D011401:Promoter Regions, Genetic / Q000235:genetics; D012441:Saccharomyces cerevisiae; D029701:Saccharomyces cerevisiae Proteins; D013053:Spectrophotometry / Q000379:methods*
D005656:Fungal Proteins; D010445:Peptide Elongation Factors; D029701:Saccharomyces cerevisiae Proteins; C111584:YEF3 protein, S cerevisiae; D000255:Adenosine Triphosphate; D000426:Alcohol Dehydrogenase