Development and optimization of a purification strategy for the venom of the scorpion Parabuthus transvaalicus.
In search for the toxic components constituting the venom of the southern African scorpion Parabuthus transvaalicus we compared and optimized several chromatographic separation protocols resulting in an efficient venom purification strategy. We found that the sequential combination of gelfiltration (Superdex) together with reversed phase chromatography (C2/C18)-but not cation exchange (Mono S)-stood surety for the best purification.
Swerts
A
A
Laboratory of Toxicology, University of Leuven, Belgium.
Debont
T
T
Verdonck
F
F
van der Walt
J J
JJ
Daenens
P
P
Tytgat
J
J
D002850:Chromatography, Gel; D002852:Chromatography, Ion Exchange; D005612:Freeze Drying; D012604:Scorpion Venoms / Q000737:chemistry*
D012604:Scorpion Venoms