Quercetin and myricetin protect against hydrogen peroxide-induced DNA damage (strand breaks and oxidised pyrimidines) in human lymphocytes.
The effects of the flavonoids quercetin and myricetin, and the antihepatotoxic agent silymarin, on hydrogen peroxide-mediated DNA damage in human lymphocytes were determined using alkaline single-cell gel electrophoresis (the comet assay). Treatment with hydrogen peroxide increased the levels of DNA strand breaks and oxidised pyrimidine bases in these cells. Quercetin was protective at concentrations above 10 microM and myricetin decreased oxidant-induced DNA strand breakage at concentrations of 100 microM. Cellular metabolism may alter the antioxidant efficacy of the flavonoids. Silymarin had no protective effect at any of the concentrations tested. None of these flavonoids was itself genotoxic. Neither alpha-tocopherol nor beta-carotene decreased hydrogen peroxide-induced DNA breakage. The differences in effectiveness of these dietary compounds against oxidative DNA damage may be explained by differences in their chemical structure or location within the cell.
Duthie
S J
SJ
Rowett Research Institute, Bucksburn, Aberdeen, Scotland, UK. sd@rri.sari.ac.uk
Collins
A R
AR
Duthie
G G
GG
Dobson
V L
VL
D002452:Cell Count / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D004249:DNA Damage / Q000187:drug effects*; D004277:DNA, Single-Stranded / Q000187:drug effects; D004587:Electrophoresis, Agar Gel / Q000379:methods; D005419:Flavonoids / Q000494:pharmacology*; D005978:Glutathione / Q000187:drug effects / Q000378:metabolism; D005980:Glutathione Reductase; D006801:Humans; D006861:Hydrogen Peroxide / Q000633:toxicity; D008214:Lymphocytes / Q000187:drug effects* / Q000201:enzymology; D010084:Oxidation-Reduction; D010544:Peroxidases / Q000187:drug effects / Q000378:metabolism; D011743:Pyrimidines / Q000378:metabolism; D011794:Quercetin / Q000494:pharmacology*; D012838:Silymarin / Q000494:pharmacology
D004277:DNA, Single-Stranded; D005419:Flavonoids; D011743:Pyrimidines; D012838:Silymarin; C040015:myricetin; D011794:Quercetin; D006861:Hydrogen Peroxide; D010544:Peroxidases; D005980:Glutathione Reductase; D005978:Glutathione