Apoptosis enhanced by soluble factor produced in feline immunodeficiency virus infection.
Feline immunodeficiency virus (FIV)-infected cells have been shown to undergo apoptosis by treatment with tumor necrosis factor alpha (TNF-alpha). This study detected a soluble factor which enhanced the apoptosis induced by TNF-alpha treatment. The sensitivity to TNF-alpha in the induction of apoptosis in a feline fibroblastoid cell line (CRFK) cells was significantly enhanced when the culture supernatant of FIV-infected CRFK cells or plasma samples from FIV-infected cats was added to the culture. These findings suggested that FIV infection induces production of a soluble factor which enhances CRFK cells sensitivity to TNF-alpha induction of apoptosis both in vitro and in vivo. This factor may contribute to the loss of lymphocytes in cats infected with FIV.
Mizuno
T
T
Department of Veterinary Internal Medicine, Faculty of Agriculture, University of Tokyo, Japan.
Momoi
Y
Y
Endo
Y
Y
Nishimura
Y
Y
Goto
Y
Y
Ohno
K
K
Watari
T
T
Tsujimoto
H
H
Hasegawa
A
A
D000818:Animals; D017209:Apoptosis / Q000187:drug effects / Q000502:physiology*; D002415:Cats; D002460:Cell Line; D003470:Culture Media; D053938:DNA Fragmentation; D003609:Dactinomycin / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D005347:Fibroblasts; D006801:Humans; D016089:Immunodeficiency Virus, Feline / Q000502:physiology*; D011994:Recombinant Proteins / Q000494:pharmacology
D003470:Culture Media; D011994:Recombinant Proteins; D003609:Dactinomycin