Buffer additives other than the surfactant sodium dodecyl sulfate for protein separations by capillary electrophoresis.
The different compounds utilized as additives to the electrolyte solutions employed in protein capillary zone electrophoresis (CZE) for minimizing protein-capillary wall interactions, for improving selectivity and resolution and for controlling the electroosmotic flow are reviewed. The dependence of the electroosmotic flow on the different variables that can be affected by the incorporation of an additive into the electrolytic solution is discussed. A list of the most effective additives employed for protein separations by CZE is reported in Appendix A.
Corradini
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Istituto di Cromatografia del CNR Area della Ricerca di Roma, Rome, Italy.
D000327:Adsorption; D000588:Amines / Q000737:chemistry; D002021:Buffers; D019075:Electrophoresis, Capillary / Q000379:methods*; D006863:Hydrogen-Ion Concentration; D009994:Osmolar Concentration; D011108:Polymers / Q000737:chemistry; D011506:Proteins / Q000737:chemistry*; D012492:Salts / Q000737:chemistry; D013499:Surface Properties; D013501:Surface-Active Agents / Q000737:chemistry; D014783:Viscosity
D000588:Amines; D002021:Buffers; D011108:Polymers; D011506:Proteins; D012492:Salts; D013501:Surface-Active Agents