Caffeine-induced disturbances of early neurogenesis in whole mouse embryo cultures.
In toto mouse embryos were cultivated at embryonic day 8.5 for 26 h with 105, 310 or 620 microM caffeine; 105-310 microM correspond to concentrations transferred by the placenta of heavy caffeine consumers. Failure of neural tube closure, excessive proliferation of neuroepithelial cells and premature evagination of telencephalic vesicles were present in 50% of treated embryos. When reaching the embryonic neural tube before neuronal migration, caffeine regionally modifies the schedule and/or rate of neural cell proliferation.
Marret
S
S
Service de Médecine Néonatale, Centre Hospitalier Universitaire de Rouen, France.
Gressens
P
P
Van-Maele-Fabry
G
G
Picard
J
J
Evrard
P
P
D000014:Abnormalities, Drug-Induced; D000818:Animals; D002110:Caffeine / Q000633:toxicity*; D004622:Embryo, Mammalian / Q000187:drug effects / Q000502:physiology*; D005314:Embryonic and Fetal Development / Q000187:drug effects*; D005260:Female; D005865:Gestational Age; D008431:Maternal-Fetal Exchange; D051379:Mice; D008815:Mice, Inbred Strains; D009420:Nervous System / Q000187:drug effects / Q000196:embryology*; D009436:Neural Tube Defects; D009924:Organ Culture Techniques; D010920:Placenta; D011247:Pregnancy
D002110:Caffeine