High-performance liquid chromatographic determination of 2-furaldehyde and 5-hydroxymethyl-2-furaldehyde in fruit juices.
A method for the determination of 2-furaldehyde (F) and 5-hydroxymethyl-2-furaldehyde (HMF) in fruit juices by high-performance liquid chromatography (HPLC) is described. The method is based on the formation of the 2,4-dinitrophenylhydrazones of carbonyl compounds and subsequent separation of these derivatives. Derivatization is carried out by utilizing an acidic solution of 2,4-dinitrophenylhydrazine in acetonitrile. Precipitation of the derivatives of carbonyl compounds is thus avoided, and direct injection of the sample into the HPLC system is allowed. The procedure offers a high specificity and a detection limit of the order of 10(-8) mol/L. Recoveries of 95-98% are obtained from apple juice spiked at different levels with both analytes. The reproducibility (mean of six determinations) is +/- 2% for F and +/- 3% for HMF.
Lo Coco
F
F
Dipartimento di Scienze e Tecnologie Chimiche, University of Udine, Italy.
Novelli
V
V
Valentini
C
C
Ceccon
L
L
D001628:Beverages / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D005519:Food Preservation / Q000592:standards; D005638:Fruit / Q000737:chemistry*; D005662:Furaldehyde / Q000032:analysis* / Q000737:chemistry; D015203:Reproducibility of Results; D019685:Rosales; D012680:Sensitivity and Specificity
D005662:Furaldehyde