One-step purification of nisin A by immunoaffinity chromatography.
The lantibiotic nisin A was purified to homogeneity by a single-step immunoaffinity chromatography method. An immunoadsorption matrix was developed by direct binding of anti-nisin A monoclonal antibodies to N-hydroxysuccinimide-activated Sepharose. The purification procedure was rapid and reproducible and rendered much higher final yields of nisin than any other described method.
Suárez
A M
AM
Departamento de Nutrición y Bromatología III, Facultad de Veterinaria, Universidad Complutense, Madrid, Spain. anagea@eucmax.sim.ucm.es
Azcona
J I
JI
Rodríguez
J M
JM
Sanz
B
B
Hernández
P E
PE
D000900:Anti-Bacterial Agents / Q000276:immunology / Q000302:isolation & purification*; D000911:Antibodies, Monoclonal; D002846:Chromatography, Affinity / Q000379:methods; D005069:Evaluation Studies as Topic; D013294:Lactococcus lactis / Q000737:chemistry; D009561:Nisin / Q000276:immunology / Q000302:isolation & purification*
D000900:Anti-Bacterial Agents; D000911:Antibodies, Monoclonal; D009561:Nisin; C102526:nisin A