Rapid discrimination of Mycobacterium tuberculosis complex strains by ligation-mediated PCR fingerprint analysis.
A ligation-mediated PCR (LMPCR) method for the amplification of sequences flanking the IS6110 of the Mycobacterium tuberculosis complex has been developed. The method uses one primer specific for IS6110 and a second specific for a linker ligated to SalI-restricted genomic DNA. LMPCR is a rapid screening method, valuable for the fingerprinting of M. tuberculosis complex strains.
Prod'hom
G
G
Centre National de Référence des Mycobactéries, Institut Pasteur, Paris, France.
Guilhot
C
C
Gutierrez
M C
MC
Varnerot
A
A
Gicquel
B
B
Vincent
V
V
D015373:Bacterial Typing Techniques / Q000706:statistics & numerical data; D001483:Base Sequence; D016172:DNA Fingerprinting / Q000379:methods* / Q000706:statistics & numerical data; D011088:DNA Ligases; D017931:DNA Primers / Q000235:genetics; D004269:DNA, Bacterial / Q000235:genetics / Q000302:isolation & purification; D005069:Evaluation Studies as Topic; D006801:Humans; D009169:Mycobacterium tuberculosis / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification; D016133:Polymerase Chain Reaction / Q000379:methods* / Q000706:statistics & numerical data; D012150:Polymorphism, Restriction Fragment Length; D012680:Sensitivity and Specificity
D017931:DNA Primers; D004269:DNA, Bacterial; D011088:DNA Ligases