Measurement of immunoglobulin synthesis using the ELISPOT assay.
We describe a method for the detection of specific antibody-producing cells from either in vitro or in vivo immunization. These techniques are especially useful for detecting antibodies from developing hybridomas. We have successfully used the system to detect isotype-specific antibodies to a variety of bacterial antigens which were produced by heterohybridomas.
Cooper
M D
MD
Department of Medical Microbiology and Immunology, Southern Illinois University School of Medicine, Springfield 62794-1220, USA. mcooper@wpsmtp.siumed.edu
Kirkpatrick
R
R
D000911:Antibodies, Monoclonal / Q000032:analysis; D000942:Antigens, Bacterial / Q000276:immunology; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D006801:Humans; D006825:Hybridomas / Q000166:cytology / Q000276:immunology; D007136:Immunoglobulins / Q000032:analysis* / Q000096:biosynthesis*
D000911:Antibodies, Monoclonal; D000942:Antigens, Bacterial; D007136:Immunoglobulins