Transplantation of CG4 oligodendrocyte progenitor cells in the myelin-deficient rat brain results in myelination of axons and enhanced oligodendroglial markers.
Transplantation of oligodendrocyte (Ol) progenitor cells into the central nervous system is a promising approach for the treatment of myelin disorders. This approach requires providing adequate numbers of healthy cells with myelinating potential. We recently showed the successful transplantation of Ol progenitors into the myelin-deficient (md) rat brain. In the present work, CG4 cells, a cell line with properties of Ol progenitors, were labeled with fast blue and grafted into P3-P5 pups born to carrier mothers. Examination of host brains 2 weeks posttransplant indicated that CG4 cells display a much more extensive migration capacity than their wild-type counterparts. These cells synthesized myelin components. In addition, ultrastructural analysis showed myelin formation along axons of md hosts in various brain regions, including corpus callosum, cerebellum, and brainstem. Furthermore, in situ hybridization studies performed on sagittal sections revealed extensive expression of transferrin-mRNA within the md host parenchyma. The high survival and functional features displayed by CG4 cells after transplantation, together with their striking wide distribution within the host parenchyma, as assessed by the presence of myelinated fibers in mutant hosts, emphasizes the importance of using highly motile and proliferative Ol progenitor cells. Strategies to improve the condition and life span of md rat pups are currently under investigation.
Espinosa de los Monteros
A
A
Department of Neurobiology, Mental Retardation Research Center, Neuropsychiatric Institute, UCLA School of Medicine, Los Angeles, California 90024, USA.
Zhao
P
P
Huang
C
C
Pan
T
T
Chang
R
R
Nazarian
R
R
Espejo
D
D
de Vellis
J
J
D000818:Animals; D001369:Axons / Q000473:pathology / Q000502:physiology*; D015415:Biomarkers; D001921:Brain / Q000166:cytology / Q000473:pathology / Q000502:physiology*; D016380:Brain Tissue Transplantation; D002460:Cell Line; D003711:Demyelinating Diseases / Q000235:genetics / Q000473:pathology / Q000628:therapy*; D006579:Heterozygote; D009186:Myelin Sheath / Q000473:pathology / Q000502:physiology*; D009836:Oligodendroglia / Q000166:cytology / Q000502:physiology / Q000637:transplantation*; D012333:RNA, Messenger / Q000096:biosynthesis; D051381:Rats; D017208:Rats, Wistar; D013234:Stem Cells; D014168:Transferrin / Q000096:biosynthesis
D015415:Biomarkers; D012333:RNA, Messenger; D014168:Transferrin