Dissecting the assembly pathway of the 20S proteasome.
Proteasomes reach their mature active state via a complex cascade of folding, assembly and processing events. The Rhodococcus proteasome offers a means to dissect the assembly pathway and to characterize intermediates; its four subunits (alpha1, alpha2, beta1, beta2) assemble efficiently in vitro with any combination of alpha and beta. Assembly studies with wild-type and N-terminally truncated beta-subunits in conjunction with refolding studies allowed to define the role of the propeptide which is two-fold: It supports the initial folding of the beta-subunits and it promotes the maturation of the holoproteasomes.
Zühl
F
F
Max-Planck-Institute for Biochemistry, Martinsried, Germany.
Seemüller
E
E
Golbik
R
R
Baumeister
W
W
D003001:Cloning, Molecular; D003546:Cysteine Endopeptidases / Q000096:biosynthesis* / Q000737:chemistry / Q000648:ultrastructure; D004792:Enzyme Precursors / Q000737:chemistry; D007700:Kinetics; D046911:Macromolecular Substances; D008854:Microscopy, Electron; D008958:Models, Molecular; D009097:Multienzyme Complexes / Q000096:biosynthesis* / Q000737:chemistry / Q000648:ultrastructure; D016133:Polymerase Chain Reaction; D046988:Proteasome Endopeptidase Complex; D011487:Protein Conformation; D011489:Protein Denaturation; D017510:Protein Folding; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000737:chemistry; D012240:Rhodococcus / Q000201:enzymology*; D016324:Sequence Tagged Sites; D013379:Substrate Specificity
D004792:Enzyme Precursors; D046911:Macromolecular Substances; D009097:Multienzyme Complexes; D011994:Recombinant Proteins; D003546:Cysteine Endopeptidases; D046988:Proteasome Endopeptidase Complex