The effects of bovine serum albumin and fetal bovine serum on the development of pre- and postcleavage-stage bovine embryos cultured in modified CR2 and M199 media.
The effects of protein supplementation on bovine embryo development in vitro was evaluated using a 4 x 2 factorial arrangement with ten replications. A total of 6438 oocytes collected from abattoir ovaries were used. Bovine serum albumin (BSA) and fetal bovine serum (FBS) were added in various combinations to simple (modified CR2) and complex (M199) media during culture of precleavage-stage IVM/IVF-derived ova from 18 h after insemination to 72 h and postcleavage-stage embryos after 72 h of culture. Cleavage rates did not differ (p > 0.05) between media supplemented with FBS or with BSA. However, the postcleavage development to the blastocyst stage of in vitro-derived bovine embryos is better in media supplemented with FBS than BSA. A greater (p < 0.05) proportion of cleaved oocytes developed to blastocysts and hatched blastocysts in media supplemented with FBS during postcleavage culture. The percentage of embryos that stopped development at the morula stage was significantly (p < 0.05) greater in media supplemented with BSA during postcleavage culture. Viability of blastocysts produced in CR2 and M199 supplemented with FBS were further assessed by transfer to recipients. In CR2, 25 transferred blastocysts resulted in seven pregnancies and the birth of three normal calves. In M199, 24 transferred blastocysts resulted in five pregnancies and the birth of two normal calves. There was no difference (p > 0.05) in rate of embryo development between CR2 and M199.
Wang
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Department of Animal, Dairy and Veterinary Sciences, Utah State University, Logan 84322-9400, USA. swang@cc.usu.edu
Liu
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Holyoak
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Bunch
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D000818:Animals; D001798:Blood Proteins / Q000378:metabolism / Q000494:pharmacology*; D002417:Cattle / Q000196:embryology*; D003470:Culture Media / Q000494:pharmacology*; D004622:Embryo, Mammalian / Q000166:cytology; D005314:Embryonic and Fetal Development / Q000187:drug effects* / Q000502:physiology; D005260:Female; D005307:Fertilization in Vitro / Q000379:methods / Q000662:veterinary*; D005333:Fetus / Q000378:metabolism; D011247:Pregnancy; D018873:Pregnancy Rate; D012710:Serum Albumin, Bovine / Q000494:pharmacology*; D013997:Time Factors
D001798:Blood Proteins; D003470:Culture Media; D012710:Serum Albumin, Bovine