Syntaxin 7, a novel syntaxin member associated with the early endosomal compartment.
Members of the syntaxin family are key molecules involved in diverse vesicle docking/fusion events. We report here the molecular, biochemical, and cell biological characterizations of a novel member (syntaxin 7) of the syntaxin family. Syntaxin 7 is structurally related to all known syntaxins. Within a 79-residue region preceding the C-terminal hydrophobic tail, syntaxin 7 is 35, 34, 34, 34, 25, and 19% identical to syntaxins 1, 2, 3, 4, 5, and 6, respectively. Northern blot analysis showed that syntaxin 7 is widely expressed. Indirect immunofluorescence microscopy revealed that syntaxin 7 is primarily associated with the early endosome. In vitro binding assays established that syntaxin 7 in membrane extracts interacts with immobilized recombinant alpha-soluble N-ethylmaleimide-sensitive factor attachment proteins fused to glutathione S-transferase. Our results highlight the general importance of members of the syntaxin family in protein trafficking and provide new avenues for future functional and mechanistic studies of this first endosomal syntaxin as well as the endocytotic pathway.
Wong
S H
SH
Membrane Biology Laboratory, Institute of Molecular and Cell Biology, Singapore 119076, Singapore.
Xu
Y
Y
Zhang
T
T
Hong
W
W
D000595:Amino Acid Sequence; D000730:Androstadienes / Q000494:pharmacology; D000818:Animals; D037263:Asialoglycoprotein Receptor; D001483:Base Sequence; D002451:Cell Compartmentation; D002460:Cell Line; D004705:Endocytosis; D011992:Endosomes / Q000378:metabolism*; D006801:Humans; D008099:Liver / Q000378:metabolism; D008565:Membrane Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D008969:Molecular Sequence Data; D009419:Nerve Tissue Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D015739:Nocodazole / Q000494:pharmacology; D050765:Qa-SNARE Proteins; D051381:Rats; D017207:Rats, Sprague-Dawley; D011956:Receptors, Cell Surface / Q000378:metabolism; D011990:Receptors, Transferrin / Q000378:metabolism; D017386:Sequence Homology, Amino Acid; D000077191:Wortmannin
D000730:Androstadienes; D037263:Asialoglycoprotein Receptor; D008565:Membrane Proteins; D009419:Nerve Tissue Proteins; D050765:Qa-SNARE Proteins; D011956:Receptors, Cell Surface; D011990:Receptors, Transferrin; D015739:Nocodazole; D000077191:Wortmannin