[Inhibition of experimental autoimmune uveoretinitis by transforming growth factor-beta 1 in B10. A mice].
Experimental autoimmune uveoretinitis (EAU) in mice, an organ specific autoimmune disease, has been investigated as an animal model for human endogenous uveitis. In this study, we report on the immunosuppressive effect of transforming growth factor-beta 1 (TGF-beta 1) on the development of EAU in mice. Inhibition by TGF-beta 1 of proliferation of interphotoreceptor retinoid-binding protein (IRBP)-specific T cell lines in B10.A mice against IRBP antigen was dose-dependent. However, when spleen cells used as the antigen presenting cell were first cultured with TGF-beta 1, this anti-proliferation effect was abolished. When IRBP-immunized mice were injected intraperitoneally with TGF-beta 1, dose-dependent suppression of EAU was obtained. The proliferation response of lymph node cells from TGF-beta 1 injected mice with IRBP-induced EAU was suppressed compared with phosphate buffered saline (PBS)-injected mice. These findings suggest that TGF-beta 1 may be a cytokine that plays a role in suppressing IRBP induced EAU in mice.
Kezuka
T
T
Department of Ophthalmology, Tokyo Medical College, Japan.
Sakai
J
J
Yokoi
H
H
Fukai
T
T
Usui
M
M
D000818:Animals; D000938:Antigen-Presenting Cells / Q000187:drug effects; D001327:Autoimmune Diseases / Q000517:prevention & control*; D002478:Cells, Cultured; D004195:Disease Models, Animal; D005136:Eye Proteins / Q000276:immunology; D005260:Female; D007166:Immunosuppressive Agents / Q000627:therapeutic use*; D051379:Mice; D008815:Mice, Inbred Strains; D012173:Retinitis / Q000517:prevention & control*; D012177:Retinol-Binding Proteins / Q000276:immunology; D013047:Specific Pathogen-Free Organisms; D016212:Transforming Growth Factor beta / Q000627:therapeutic use*; D014605:Uveitis / Q000517:prevention & control*; D000099214:Retinol-Binding Proteins, Interstitial
D005136:Eye Proteins; D007166:Immunosuppressive Agents; D012177:Retinol-Binding Proteins; D016212:Transforming Growth Factor beta; D000099214:Retinol-Binding Proteins, Interstitial