Detection of the Sec-1 locus of rye by a PCR-based method.
The structural genes for the omega-secalins of rye (Secale cereale) are located in the Sec-1 locus on the short arm of rye chromosome 1R. We applied PCR (polymerase chain reaction) to detect the Sec-1 locus in a wheat genomic background. A primer set we designed based on a published sequence of a omega-secalin gene amplified not only the omega-secalin sequence, but also a putative omega-gliadin sequence. We determined partial sequences of both PCR-amplified fragments and designed different primers for the specific amplification of the omega-secalin sequence. One of the new primer sets amplified DNA fragments only in rye and wheat lines carrying chromosome 1R or telosome 1RS; no amplification occurred in either euploid wheats or 1RS deletion lines. This PCR-based method would provide efficient screening for the Sec-1 locus in progeny of wheat lines carrying chromosome 1R.
Shimizu
Y
Y
Laboratory of Plant Genetics, Graduate School of Agriculture, Kyoto University, Japan.
Nasuda
S
S
Endo
T R
TR
D001483:Base Sequence; D003001:Cloning, Molecular; D017931:DNA Primers; D005983:Glutens; D008969:Molecular Sequence Data; D010940:Plant Proteins / Q000235:genetics*; D016133:Polymerase Chain Reaction / Q000379:methods*; D015183:Restriction Mapping; D012434:Secale / Q000235:genetics*; D012680:Sensitivity and Specificity; D017422:Sequence Analysis, DNA; D014908:Triticum / Q000235:genetics
D017931:DNA Primers; D010940:Plant Proteins; D005983:Glutens