title: Immunogenicity of low-dose intramuscular and intradermal vaccination with recombinant hepatitis B vaccine.
abstract: The study is a randomized trial using recombinant DNA vaccine to determine whether an intramuscular 10 micrograms dose or intradermal 2 micrograms induces satisfactory anti-HBs levels compared to the standard dose of intramuscular 20 micrograms. Participants were 359 healthy medical and nurse students randomly allocated to one of the three groups: Group I-IM 20 micrograms; Group II-IM 10 micrograms; Group III-ID 2 micrograms at 0, 1 and 6 months. Anti-HBs titres were measured after complete vaccine schedule by ELISA/Pasteur. Baseline variables were similar among groups and side effects were mild after any dose. Vaccines in the IM-10 micrograms group had seroconversion rate and geometric mean titre (GMT 2344 IU L-1), not significant different from the IM-20 micrograms group (GMT 4570 IU L-1). On the contrary, 21.4% of the ID-2 micrograms recipients mount antibody concentration below 10 IU L-1 and GMT of 91 IU L-1, a statistically significant difference compared with the standard schedule IM-20 micrograms (p < 0.001). A three dose regimen of half dose IM could be considered an appropriate schedule to prevent hepatitis B in young health adults which is of relevance to the expansion of hepatitis B vaccine programme.
authors.affiliation: Universidade Federal de Goiás, Goiânia, Brasil.
authors.forename: M D
authors.initials: MD
authors.lastname: Turchi
authors.forename: C M
authors.initials: CM
authors.lastname: Martelli
authors.forename: M L
authors.initials: ML
authors.lastname: Ferraz
authors.forename: A E
authors.initials: AE
authors.lastname: Silva
authors.forename: D
authors.initials: D
authors.lastname: Cardoso D das
authors.forename: P
authors.initials: P
authors.lastname: Martelli
authors.forename: L J
authors.initials: LJ
authors.lastname: Oliveira
mesh_terms: D000293:Adolescent; D000328:Adult; D005260:Female; D006509:Hepatitis B / Q000517:prevention & control*; D017325:Hepatitis B Vaccines / Q000008:administration & dosage* / Q000276:immunology*; D006801:Humans; D008297:Male
chemical_list: D017325:Hepatitis B Vaccines