DNA typing of human leukocyte antigen sequence polymorphisms by peptide nucleic acid probes and MALDI-TOF mass spectrometry.
A novel analytical method using PNA probes detected by matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOFMS) was applied to type sequence polymorphisms within the human leukocyte antigen (HLA), DQA locus. Streptavidin-coated magnetic beads were used to immobilize biotinylated DNA. PNA probes representing possible alleles were then prepared for the immobilized DNA hybridization. The nonspecific PNA probes were removed with stringent washes. The PNA/DNA/beads conjugate was analyzed by MALDI-TOFMS. The genotype of the DNA was determined by the detected molecular masses of the released PNA probes. Reproducible and accurate genotyping was achieved by this analytical method.
Jiang-Baucom
P
P
Department of Chemistry, American University, Washington, D.C. 20016, USA.
Girard
J E
JE
Butler
J
J
Belgrader
P
P
D004247:DNA / Q000737:chemistry*; D006680:HLA Antigens / Q000737:chemistry*; D006801:Humans; D015345:Oligonucleotide Probes / Q000737:chemistry*; D011110:Polymorphism, Genetic; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
D006680:HLA Antigens; D015345:Oligonucleotide Probes; D004247:DNA