title: New biomarker evidence of oxidative DNA damage in patients with non-insulin-dependent diabetes mellitus.
abstract: Urinary 8-hydroxy-2'-deoxyguanosine (8-OHdG) has been reported to serve as a sensitive biomarker of oxidative DNA damage and also of oxidative stress. We have investigated oxidative DNA damage in patients with non-insulin-dependent diabetes mellitus (NIDDM) by urinary 8-OHdG assessments. We determined the total urinary excretion of 8-OHdG from 24 h urine samples of 81 NIDDM patients 9 years after the initial diagnosis and of 100 non-diabetic control subjects matched for age and gender. The total 24 h urinary excretion of 8-OHdG was markedly higher in NIDDM patients than in control subjects (68.2 +/- 39.4 microg vs. 49.6 +/- 37.7 microg, P = 0.001). High glycosylated hemoglobin was associated with a high level of urinary 8-OHdG. The increased excretion of urinary 8-OHdG is seen as indicating an increased systemic level of oxidative DNA damage in NIDDM patients.
authors.affiliation: Laboratory of Neurobiology, Medical School, University of Tampere, Finland.
authors.forename: J
authors.initials: J
authors.lastname: Leinonen
authors.forename: T
authors.initials: T
authors.lastname: Lehtimäki
authors.forename: S
authors.initials: S
authors.lastname: Toyokuni
authors.forename: K
authors.initials: K
authors.lastname: Okada
authors.forename: T
authors.initials: T
authors.lastname: Tanaka
authors.forename: H
authors.initials: H
authors.lastname: Hiai
authors.forename: H
authors.initials: H
authors.lastname: Ochi
authors.forename: P
authors.initials: P
authors.lastname: Laippala
authors.forename: V
authors.initials: V
authors.lastname: Rantalaiho
authors.forename: O
authors.initials: O
authors.lastname: Wirta
authors.forename: A
authors.initials: A
authors.lastname: Pasternack
authors.forename: H
authors.initials: H
authors.lastname: Alho
mesh_terms: D000080242:8-Hydroxy-2'-Deoxyguanosine; D000368:Aged; D015415:Biomarkers / Q000652:urine; D001786:Blood Glucose / Q000378:metabolism; D004249:DNA Damage; D003849:Deoxyguanosine / Q000031:analogs & derivatives* / Q000378:metabolism / Q000652:urine; D003924:Diabetes Mellitus, Type 2 / Q000378:metabolism / Q000652:urine*; D005260:Female; D006442:Glycated Hemoglobin / Q000378:metabolism; D006801:Humans; D008297:Male; D008875:Middle Aged; D018384:Oxidative Stress; D012737:Sex Factors; D012907:Smoking
chemical_list: D000080242:8-Hydroxy-2'-Deoxyguanosine; D015415:Biomarkers; D001786:Blood Glucose; D003849:Deoxyguanosine; D006442:Glycated Hemoglobin