A first-generation whole genome-radiation hybrid map spanning the mouse genome.
We have assembled a first-generation anchor map of the mouse genome using a panel of 94 whole-genome-radiation hybrids (WG-RHs) and 271 sequence-tagged sites (STSs). This is the first genome-wide RH anchor map of a model organism. All of the STSs have been previously localized on the genetic map and are located 8.8 Mb apart on average. This mouse WG-RH panel, known as T31, has an average retention frequency of 27.6% and an estimated potential resolution of 145 kb, making it a powerful resource for efficient large-scale expressed sequence tag mapping. [All of the mapping data for the maps presented here have been deposited at the Research Genetics, Inc., web site and can be freely accessed and downloaded at http://www.resgen.com/.]
McCarthy
L C
LC
Genetics Department, University of Cambridge, Downing Site, Cambridge CB2 3EH, UK. lmc@mole.bio.cam.ac.uk
Terrett
J
J
Davis
M E
ME
Knights
C J
CJ
Smith
A L
AL
Critcher
R
R
Schmitt
K
K
Hudson
J
J
Spurr
N K
NK
Goodfellow
P N
PN
D000818:Animals; D002460:Cell Line; D002874:Chromosome Mapping; D002875:Chromosomes / Q000235:genetics*; D006224:Cricetinae; D018076:DNA, Complementary; D005347:Fibroblasts; D015870:Gene Expression; D005819:Genetic Markers; D006822:Hybrid Cells / Q000528:radiation effects / Q000648:ultrastructure; D017404:In Situ Hybridization, Fluorescence; D051379:Mice; D016133:Polymerase Chain Reaction; D016324:Sequence Tagged Sites; D013234:Stem Cells; D014965:X-Rays
D018076:DNA, Complementary; D005819:Genetic Markers