Thrombin has a bimodal effect on glioma cell growth.
Using rat glioma C6 cells as a model, we have found a bimodal effect of alpha-thrombin on cell growth. In C6 cells treated with alpha-thrombin at concentrations from 0.02 nM to 1.0 nM, inhibition of cell proliferation was noted. Because the thrombin receptor agonist peptide TRAP-6 also induced inhibition of cell proliferation and the thrombin receptor antagonist peptide T1 prevented the inhibitory effect of alpha-thrombin on C6 glioma cell growth, thrombin receptor involvement in antiproliferative action of alpha-thrombin in C6 glioma cells is highly likely. However, stimulation of cell proliferation observed when C6 cells were treated with alpha-thrombin at higher doses (> 1.0 nM) seems to be mediated by as yet undefined thrombin receptor-independent biochemical mechanisms.
Schafberg
H
H
Max Planck Gesellschaft, Research Unit Pharmacological Hemostaseology at the Friedrich Schiller University Jena, Germany.
Nowak
G
G
Kaufmann
R
R
D000818:Animals; D002455:Cell Division / Q000187:drug effects; D004247:DNA / Q000096:biosynthesis; D005910:Glioma / Q000473:pathology*; D010446:Peptide Fragments / Q000494:pharmacology; D051381:Rats; D018179:Receptors, Thrombin / Q000187:drug effects / Q000502:physiology; D013917:Thrombin / Q000494:pharmacology*; D014407:Tumor Cells, Cultured
D010446:Peptide Fragments; D018179:Receptors, Thrombin; C082835:thrombin receptor peptide (42-47); D004247:DNA; D013917:Thrombin