title: Absence of human herpesvirus 8 and Epstein-Barr virus genome sequences in cutaneous epithelial neoplasms arising in immunosuppressed organ-transplant patients.
abstract: Recent studies have implicated herpesvirus 8 and Epstein-Barr virus in the development of cutaneous malignancies in immunosuppressed patients. In order to examine the strength of this association, we examined 37 malignant, pre-malignant and benign cutaneous epithelial neoplasms removed from immunosuppressed organ recipients for the presence of human herpesvirus 8 and Epstein-Barr viral genome sequences using polymerase chain reaction (PCR) and in situ hybridization. We examined 2 actinic keratoses, 1 benign keratosis, 11 invasive squamous cell carcinomas, 17 squamous cell carcinomas in situ and 6 basal cell carcinomas. We also examined 4 basal cell carcinomas, 1 invasive squamous cell carcinoma and 3 squamous cell carcinomas in immunocompetent hosts. In contrast to findings reported by other investigators, we were unable to detect viral genome sequences in any of the biopsies examined. Our findings suggest that human herpesvirus 8 and Epstein-Barr virus likely do not play an etiologic role in cutaneous epithelial oncogenesis in immunocompromised patients.
authors.affiliation: Department of Pathology, Stanford University Medical Center, California 94305, USA.
authors.forename: S
authors.initials: S
authors.lastname: Kohler
authors.forename: O W
authors.initials: OW
authors.lastname: Kamel
authors.forename: P P
authors.initials: PP
authors.lastname: Chang
authors.forename: B R
authors.initials: BR
authors.lastname: Smoller
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D001706:Biopsy; D002278:Carcinoma in Situ / Q000276:immunology / Q000821:virology; D002280:Basal Cell Carcinoma / Q000276:immunology / Q000821:virology; D002294:Carcinoma, Squamous Cell / Q000276:immunology / Q000821:virology*; D004279:DNA, Viral / Q000032:analysis; D004848:Epithelium / Q000473:pathology / Q000821:virology; D005260:Female; D016679:Genome, Viral; D004854:Herpesvirus 4, Human / Q000235:genetics / Q000302:isolation & purification*; D019288:Herpesvirus 8, Human / Q000235:genetics / Q000302:isolation & purification*; D006801:Humans; D007165:Immunosuppression Therapy; D017403:In Situ Hybridization; D007642:Keratosis / Q000821:virology; D008297:Male; D008875:Middle Aged; D016133:Polymerase Chain Reaction; D012367:RNA, Viral / Q000032:analysis; D012878:Skin Neoplasms / Q000276:immunology / Q000821:virology*; D014181:Transplantation Immunology
chemical_list: D004279:DNA, Viral; D012367:RNA, Viral