title: Restriction fragment length polymorphism of Mycobacterium tuberculosis strains from various regions of India, using direct repeat probe.
abstract: Intraspecies differentiation was studied on 68 M. tuberculosis strains obtained from 6 states of India by restriction fragment length polymorphism (RFLP) using a direct repeat probe (DR probe) hybridised with Alu I digest of DNA. Most strains showed polymorphism based patterns that comprised between 2 to 7 bands and were grouped into 26 RFLP types. Of the 11 strains tested from Amritsar, 8 were RFLP type 5; the remaining 3 were of type 11 and were exclusively confined to this region. The strains from other regions were more heterogeneous. We confirm that DR-associated RFLP can be an excellent tool for the differentiation of M. tuberculosis strains. Depending on their geographical origin, these strains can be differentiated to a large extent by DR fingerprinting.
authors.affiliation: Tuberculosis Research Center (ICMR), Chennai.
authors.forename: S
authors.initials: S
authors.lastname: Narayanan
authors.forename: R
authors.initials: R
authors.lastname: Sahadevan
authors.forename: P R
authors.initials: PR
authors.lastname: Narayanan
authors.forename: P V
authors.initials: PV
authors.lastname: Krishnamurthy
authors.forename: C N
authors.initials: CN
authors.lastname: Paramasivan
authors.forename: R
authors.initials: R
authors.lastname: Prabhakar
mesh_terms: D016172:DNA Fingerprinting; D004269:DNA, Bacterial / Q000032:analysis*; D009169:Mycobacterium tuberculosis / Q000235:genetics*; D012150:Polymorphism, Restriction Fragment Length; D012091:Repetitive Sequences, Nucleic Acid
chemical_list: D004269:DNA, Bacterial