title: A novel ets-related transcription factor, ERT/ESX/ESE-1, regulates expression of the transforming growth factor-beta type II receptor.
abstract: A 2.5-kilobase cDNA clone that encodes a 371-amino acid novel transcription factor was isolated from a human placenta cDNA library using a yeast one-hybrid system. The novel ets-related transcription factor (ERT) showed a homology with the ETS DNA-binding domain. Using constructs of the transforming growth factor-beta (TGF-beta) type II receptor (RII) promoter linked to the luciferase gene, we have demonstrated that ERT activates transcription of the TGF-beta RII gene through the 5'-TTTCCTGTTTCC-3' response element spanning nucleotides +13 to +24 and multiple additional ETS binding sites between -1816 and -82 of the TGF-beta RII promoter. A specific interaction between ERT and the ETS binding sites was also demonstrated using an electrophoretic mobility shift assay. Deletion mapping of ERT protein suggests that the transactivation domain resides in the amino terminus while the DNA-binding domain is localized to the carboxyl-terminal region. Our results suggest that ERT might be a major transcription factor involved in the transcriptional regulation of the TGF-beta RII gene.
authors.affiliation: Laboratory of Cell Regulation and Carcinogenesis, NCI, National Institutes of Health, Bethesda, Maryland 20892-5055, USA.
authors.forename: S G
authors.initials: SG
authors.lastname: Choi
authors.forename: Y
authors.initials: Y
authors.lastname: Yi
authors.forename: Y S
authors.initials: YS
authors.lastname: Kim
authors.forename: M
authors.initials: M
authors.lastname: Kato
authors.forename: J
authors.initials: J
authors.lastname: Chang
authors.forename: H W
authors.initials: HW
authors.lastname: Chung
authors.forename: K B
authors.initials: KB
authors.lastname: Hahm
authors.forename: H K
authors.initials: HK
authors.lastname: Yang
authors.forename: H H
authors.initials: HH
authors.lastname: Rhee
authors.forename: Y J
authors.initials: YJ
authors.lastname: Bang
authors.forename: S J
authors.initials: SJ
authors.lastname: Kim
mesh_terms: D003001:Cloning, Molecular; D018076:DNA, Complementary; D005786:Gene Expression Regulation; D006801:Humans; D008969:Molecular Sequence Data; D011401:Promoter Regions, Genetic; D011485:Protein Binding; D017346:Protein Serine-Threonine Kinases; D011518:Proto-Oncogene Proteins / Q000378:metabolism*; D050783:Proto-Oncogene Proteins c-ets; D000077294:Receptor, Transforming Growth Factor-beta Type II; D018125:Receptors, Transforming Growth Factor beta / Q000235:genetics*; D014157:Transcription Factors / Q000378:metabolism*; D014158:Transcription, Genetic
chemical_list: D018076:DNA, Complementary; D011518:Proto-Oncogene Proteins; D050783:Proto-Oncogene Proteins c-ets; D018125:Receptors, Transforming Growth Factor beta; D014157:Transcription Factors; D017346:Protein Serine-Threonine Kinases; D000077294:Receptor, Transforming Growth Factor-beta Type II