title: Determinant role of E. coli RNase III in the decay of both specific and heterologous mRNAs.
abstract: A comparative analysis of mRNA decay was carried out in Escherichia coli using the wild-type and an isogenic RNase III deletion strain. We have studied the mRNA degradation from the Escherichia coli gene bolA, the Lactococcus lactis biovar diacetylactis citQRP operon and the Desulfovibrio vulgaris Hildenborough gene cyc. As seen by a dramatic stabilization of the specific mRNAs in the mutant strain, RNase III was crucial for the decay process of these three messages. Since RNase III, unlike RNase E, is not essential for bacterial viability we think that there is potential for using RNase III mutant strains to modulate gene expression.
authors.affiliation: Instituto de Tecnologia Quimica e Biológica, Universidade Nova de Lisboa, Oeiras, Portugal.
authors.forename: J M
authors.initials: JM
authors.lastname: Santos
authors.forename: D
authors.initials: D
authors.lastname: Drider
authors.forename: P E
authors.initials: PE
authors.lastname: Marujo
authors.forename: P
authors.initials: P
authors.lastname: Lopez
authors.forename: C M
authors.initials: CM
authors.lastname: Arraiano
mesh_terms: D001426:Bacterial Proteins / Q000235:genetics; D016969:Desulfovibrio vulgaris / Q000201:enzymology / Q000235:genetics; D004722:Endoribonucleases / Q000378:metabolism*; D004926:Escherichia coli / Q000201:enzymology* / Q000235:genetics*; D029968:Escherichia coli Proteins; D015964:Gene Expression Regulation, Bacterial; D015971:Gene Expression Regulation, Enzymologic; D013294:Lactococcus lactis / Q000201:enzymology / Q000235:genetics; D009154:Mutation; D009876:Operon; D010957:Plasmids; D012329:RNA, Bacterial / Q000378:metabolism; D012333:RNA, Messenger / Q000378:metabolism*; D043244:Ribonuclease III
chemical_list: D001426:Bacterial Proteins; D029968:Escherichia coli Proteins; D012329:RNA, Bacterial; D012333:RNA, Messenger; D004722:Endoribonucleases; D043244:Ribonuclease III; C468366:ribonuclease III, E coli