Neurotrophin-3 mRNA expression in rat intrafusal muscle fibres after denervation and reinnervation.
We have studied the regulation of the expression of neurotrophin-3 (NT-3) mRNA in neonatal and adult rat muscle spindles after denervation and after denervation followed by reinnervation. Denervation of the intrafusal fibres did not result in an upregulation of the NT-3 mRNA expression but decreased this expression below the detection limit of the in situ hybridization method. Reinnervation of intrafusal fibres restored the NT-3 mRNA expression. The results suggest that the expression of NT-3 mRNA in postnatal muscle spindles is controlled by neuronal factors. The intrafusal fibre derived NT-3 may act as an instructive, feedback messenger for innervating neurons and may play a role in stabilizing and maintaining functional neuromuscular connections.
Copray
J C
JC
Department of Medical Physiology, University of Groningen, Netherlands.
Brouwer
N
N
D000818:Animals; D000831:Animals, Newborn; D003714:Denervation; D018485:Muscle Fibers, Skeletal / Q000378:metabolism*; D009470:Muscle Spindles / Q000378:metabolism*; D009409:Nerve Crush; D009414:Nerve Growth Factors / Q000235:genetics*; D009416:Nerve Regeneration / Q000502:physiology*; D020933:Neurotrophin 3; D012333:RNA, Messenger / Q000096:biosynthesis*; D051381:Rats; D017208:Rats, Wistar
D009414:Nerve Growth Factors; D020933:Neurotrophin 3; D012333:RNA, Messenger