Rapid semi-quantitative estimation of N-nitrosodibutylamine and N-nitrosodibenzylamine in smoked hams by solid-phase microextraction followed by gas chromatography-thermal energy analysis.
A solid-phase microextraction (SPME) analytical method has been developed for the determination of N-nitrosodi-n-butylamine (NDBA) and N-nitrosodibenzylamine (NDBzA) in hams that is based on: (a) isolation of the compounds by steam distillation, (b) SPME from the distillate headspace using a polyacrylate coated silica fibre and (c) determination by gas chromatography-thermal energy analyzer technique or confirmation by gas chromatography-mass spectrometry. Recoveries of both NDBA and NDBzA from hams spiked at 5 to 160 micrograms/kg levels ranged between 41 to 112%. The overall method is fast, sensitive (detection limits, 1 to 3 micrograms/kg), precise (within 10%) and fairly accurate (average recoveries 86% and 70%, respectively). The results obtained by this technique for seven ham samples agreed fairly well with those obtained by an existing method (r2 = 0.97). The new method is solventless, environmentally friendly and useful for rapid monitoring purposes.
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Food Research Division, Food Directorate (2203D), Health Protection Branch, Health Canada, Ottawa, Ontario, Canada.
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D000818:Animals; D002273:Carcinogens / Q000032:analysis*; D018857:Food Packaging; D008401:Gas Chromatography-Mass Spectrometry; D007202:Indicators and Reagents; D008460:Meat / Q000032:analysis*; D008934:Mitogens / Q000032:analysis*; D009602:Nitrosamines / Q000032:analysis*; D012015:Reference Standards; D013552:Swine
D002273:Carcinogens; D007202:Indicators and Reagents; D008934:Mitogens; D009602:Nitrosamines; C015395:N-nitrosodibenzylamine; C012279:dibutylnitrosamine