title: Ribosomal efficiency and growth rates of freshly isolated Escherichia coli strains originating from the gastrointestinal tract.
abstract: It has been previously reported that for natural Escherichia coli isolates from the ECOR collection, there were differences in the ribosomal efficiencies and there was a direct correlation between growth rate and the ribosome efficiency (R-factor). The aim of this study was to determine whether strains freshly isolated (i.e. subcultured < 5 times) from the gastrointestinal tract ecosystem also exhibited this correlation. Eleven E. coli and two Enterobacter spp. isolates from either humans, pigs, rats or a mammoth were investigated. Considerable variability in the R-factor was noted using an in vitro translation assay, however no consistent correlation between the R-factor and growth rate was noted.
authors.affiliation: Department of General and Marine Microbiology, Göteborg University, Sweden. Camilla.Rang@gmm.gu.se
authors.forename: C U
authors.initials: CU
authors.lastname: Rang
authors.forename: R
authors.initials: R
authors.lastname: Mikkola
authors.forename: S
authors.initials: S
authors.lastname: Molin
authors.forename: P L
authors.initials: PL
authors.lastname: Conway
mesh_terms: D000818:Animals; D001315:Australia; D004064:Digestive System / Q000382:microbiology*; D004754:Enterobacter / Q000302:isolation & purification; D004926:Escherichia coli / Q000254:growth & development / Q000302:isolation & purification / Q000502:physiology*; D005580:Fossils; D006801:Humans; D007700:Kinetics; D008322:Mammals; D011815:R Factors; D051381:Rats; D012270:Ribosomes / Q000378:metabolism*; D013552:Swine