Comparison of electron microscopy, enzyme-linked immunosorbent assay and latex agglutination for the detection of bovine rotavirus in faeces.
The performance characteristics of 2 enzyme immunoassays (ELISAs) and 4 latex agglutination assays (LXs) were evaluated for the detection of bovine rotavirus in faecal specimens of young calves with diarrhoea. A total of 26 specimens from calves less than 5 months of age were examined with different commercial assays and compared with electron microscopy (EM) as the gold standard and with polyacrylamide gel electrophoresis (PAGE) for the detection of atypical, non-group A rotaviruses. In the 2nd study, EIA (Dako) and LX (Murex), the assays of choice, were used to analyse 97 further faecal specimens from calves with diarrhoea. The ELISAs proved to be the most sensitive compared with the other tests used. The EM and PAGE are 100% specific although slightly less sensitive than the commercial assays. The results show that all the commercial assays can accurately detect rotavirus in the stools of calves with gastroenteritis, although the suitability and choice of assay will depend upon the requirements of individual laboratories.
de Beer
M
M
MRC/Medunsa Diarrhoeal Pathogens Research Unit, Department of Virology, Medical University of Southern Africa, Medunsa, South Africa.
Peenze
I
I
da Costa Mendes
V M
VM
Steele
A D
AD
D000818:Animals; D000956:Antigens, Viral / Q000032:analysis; D002417:Cattle; D002418:Cattle Diseases / Q000175:diagnosis*; D004591:Electrophoresis, Polyacrylamide Gel; D004797:Enzyme-Linked Immunosorbent Assay; D005243:Feces / Q000821:virology*; D007841:Latex Fixation Tests; D008854:Microscopy, Electron; D012401:Rotavirus / Q000302:isolation & purification*; D012400:Rotavirus Infections / Q000175:diagnosis / Q000662:veterinary*; D012680:Sensitivity and Specificity
D000956:Antigens, Viral