Cloning of the murine tetranectin gene and 5'-flanking region.
The gene encoding murine tetranectin (Tna) and its 5'-flanking region was isolated and cloned from a EMBL3 SP6/T7 genomic library. The compiled nucleotide sequence was determined by sequencing, revealing a conserved Tna structure in man and mouse. Mapping of the transcription start point (tsp) suggests that murine Tna has more than one of these. In addition, no consensus TATA-box was found uptream for the putative tsp(s) in the 5'-flanking region of the gene, indicating that the murine Tna promoter belongs to the TATA-less class of genes. The cloned murine Tna was mapped to region F1-F3 on mouse chromosome 9 by fluorescence in situ hybridization (FISH).
Sørensen
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Protein Chemistry Laboratory, University of Aarhus, Science Park, Aarhus C, Denmark.
Berglund
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Petersen
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D000818:Animals; D001798:Blood Proteins / Q000235:genetics*; D002874:Chromosome Mapping; D003001:Cloning, Molecular; D017404:In Situ Hybridization, Fluorescence; D037181:Lectins, C-Type; D051379:Mice; D008807:Mice, Inbred BALB C; D012091:Repetitive Sequences, Nucleic Acid; D014158:Transcription, Genetic
D001798:Blood Proteins; D037181:Lectins, C-Type; C048648:tetranectin