Quantitative measurement of protein kinase C immunoreactivity in rod bipolar cells of the goldfish retina.
The intensity of the immunohistochemical reaction (IIR) against the alpha species of protein kinase C (PKC) was quantified in the rod bipolar cells (RBC) of the goldfish retina using of image analysis. Retinae incubated in control Ringer solution showed similar IIR in both the soma and the axon terminal (IIR-ratio approximately 1). Activation of PKC induces the 'transport' of the enzyme to the synaptic terminal of RBC and an increase in the IIR-ratio. In the present report, the effect of retinal neurotransmitters on the IIR-ratio and the time course of PKC transport was studied.
Vaquero
C F
CF
Department of Physiology, School of Medicine, University of Alcala, Madrid, Spain.
Velasco
A
A
de la Villa
P
P
D000818:Animals; D001369:Axons / Q000201:enzymology / Q000648:ultrastructure; D002118:Calcium / Q000494:pharmacology; D004298:Dopamine / Q000494:pharmacology; D006054:Goldfish; D007150:Immunohistochemistry; D066298:In Vitro Techniques; D007527:Isoenzymes / Q000032:analysis / Q000378:metabolism*; D007700:Kinetics; D009411:Nerve Endings / Q000201:enzymology / Q000648:ultrastructure; D009543:Nifedipine / Q000494:pharmacology; D011188:Potassium / Q000494:pharmacology; D011493:Protein Kinase C / Q000032:analysis / Q000378:metabolism*; D051571:Protein Kinase C-alpha; D011494:Protein Kinases / Q000032:analysis / Q000378:metabolism*; D012160:Retina / Q000166:cytology*; D017948:Retinal Rod Photoreceptor Cells / Q000166:cytology* / Q000201:enzymology*; D012701:Serotonin / Q000494:pharmacology; D013569:Synapses / Q000201:enzymology / Q000648:ultrastructure; D014660:Vasoactive Intestinal Peptide / Q000494:pharmacology; D005680:gamma-Aminobutyric Acid / Q000494:pharmacology
D007527:Isoenzymes; D012701:Serotonin; D014660:Vasoactive Intestinal Peptide; D005680:gamma-Aminobutyric Acid; D011494:Protein Kinases; D011493:Protein Kinase C; D051571:Protein Kinase C-alpha; D009543:Nifedipine; D011188:Potassium; D002118:Calcium; D004298:Dopamine