title: Binding of an arm repeat protein to the kinase domain of the S-locus receptor kinase.
abstract: Screening of a yeast two-hybrid library for proteins that interact with the kinase domain of an S-locus receptor kinase (SRK) resulted in the isolation of a plant protein called ARC1 (Arm Repeat Containing). This interaction was mediated by the C-terminal region of ARC1 in which five arm repeat units were identified. Using the yeast two-hybrid system and in vitro binding assays, ARC1 was found to interact specifically with the kinase domains from SRK-910 and SRK-A14 but failed to interact with kinase domains from two different Arabidopsis receptor-like kinases. In addition, treatment with a protein phosphatase or the use of a kinase-inactive mutant reduced or abolished the binding of ARC1 to the SRK-910 kinase domain, indicating that the interaction was phosphorylation dependent. Lastly, RNA blot analysis revealed that the expression of ARC1 is restricted to the stigma, the site of the self-incompatibility response.
authors.affiliation: Biology Department, York University, 4700 Keele Street, North York, Ontario M3J 1P3, Canada.
authors.forename: T
authors.initials: T
authors.lastname: Gu
authors.forename: M
authors.initials: M
authors.lastname: Mazzurco
authors.forename: W
authors.initials: W
authors.lastname: Sulaman
authors.forename: D D
authors.initials: DD
authors.lastname: Matias
authors.forename: D R
authors.initials: DR
authors.lastname: Goring
mesh_terms: D000595:Amino Acid Sequence; D017360:Arabidopsis / Q000201:enzymology; D005656:Fungal Proteins / Q000235:genetics / Q000378:metabolism; D006023:Glycoproteins / Q000378:metabolism*; D008969:Molecular Sequence Data; D010766:Phosphorylation; D010940:Plant Proteins / Q000378:metabolism*; D011494:Protein Kinases / Q000378:metabolism*; D012337:RNA, Ribosomal, 18S / Q000378:metabolism; D016601:RNA-Binding Proteins / Q000235:genetics / Q000378:metabolism; D029701:Saccharomyces cerevisiae Proteins
chemical_list: C102145:ARC1 protein, S cerevisiae; D005656:Fungal Proteins; D006023:Glycoproteins; D010940:Plant Proteins; D012337:RNA, Ribosomal, 18S; D016601:RNA-Binding Proteins; D029701:Saccharomyces cerevisiae Proteins; C064156:self-incompatibility locus specific glycoprotein, Brassica; D011494:Protein Kinases; C079832:S-receptor kinase