title: Expression of mouse RNase MRP RNA in human embryonic kidney 293 cells.
abstract: We report on the expression of mouse RNase MRP RNA in human embryonic kidney 293 cells upon DNA transfection. Stable cell lines were selected by cotransfection with a neor gene. Transcription of wild-type and deletion mutants of MRP RNA and ribonucleoprotein formation were assessed by RNase protection and immunoprecipitation experiments. Mouse MRP RNA as expressed in 293 cells readily associates with human proteins to form a chimeric Th ribonucleoprotein. 5' truncated MRP RNAs, however, failed to associate with Th antigen(s) and deletion of the 3' sequences of MRP RNA greatly reduced the expression in stable as well as in transient transfectants.
authors.affiliation: Projektgruppe Genexpression, Institut für Tumorbiologie-Krebsforschung der Universtität Wien, Austria.
authors.forename: W
authors.initials: W
authors.lastname: Rossmanith
authors.forename: E
authors.initials: E
authors.lastname: Bettinger
authors.forename: C
authors.initials: C
authors.lastname: Cerni
authors.forename: R M
authors.initials: RM
authors.lastname: Karwan
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D004722:Endoribonucleases / Q000235:genetics* / Q000378:metabolism*; D006801:Humans; D007668:Kidney / Q000166:cytology / Q000196:embryology; D051379:Mice; D009154:Mutation; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism; D012261:Ribonucleoproteins / Q000378:metabolism; D014158:Transcription, Genetic; D014162:Transfection
chemical_list: D011994:Recombinant Proteins; D012261:Ribonucleoproteins; D004722:Endoribonucleases; C058330:mitochondrial RNA-processing endoribonuclease