title: Analysis of DNA from subjects found to be heterozygous for the haptoglobin-Johnson alpha gene.
abstract: We found two rare Hp Johnson variant families (Hp2-Johnson type and Hp1-Johnson type) by PAGE. It was confirmed by SDS-PAGE that the haptoglobin molecules with the Hp Johnson variant consisted of alpha-2 or alpha-1 and alpha-3 subunits. Direct gene analysis of Hp Johnson heterozygous individuals using three endonucleases: EcoRI, HindIII and BamHI, showed a three-fold tandem repeat of the same 1.7-kb DNA fragment implicated in the Hp alpha-2 gene duplication, and this Hp-Johnson-specific extension was identified in two generations of the Hp2-Johnson and Hp1-Johnson families.
authors.affiliation: Department of Forensic Science, School of Allied Health Sciences, Kitasato University, Sagamihara-shi, Kanagawa, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Nagai
authors.forename: T
authors.initials: T
authors.lastname: Okazaki
authors.forename: Y
authors.initials: Y
authors.lastname: Yanagisawa
mesh_terms: D004247:DNA; D004591:Electrophoresis, Polyacrylamide Gel; D005260:Female; D014644:Genetic Variation; D006242:Haptoglobins / Q000235:genetics*; D006579:Heterozygote; D006801:Humans; D008297:Male; D010375:Pedigree
chemical_list: D006242:Haptoglobins; D004247:DNA