title: NKR-P1dim/TCR alpha beta + T cells and natural killer cells share expression of NKR-P1A and NKR-P1D.
abstract: Natural killer (NK) cells and a T cell subset express NKR-P1s; however, it is not known if NKR-P1s on these cells are homologous. By molecular and biochemical means, we determined that NKR-P1s on NK cells and T cells were similar. Also, sequencing of polymerase chain reaction products derived from these populations indicated expression of a novel form, termed NKR-P1D, with approximately 60% nucleotide homology to NKR-P1A. NKR-P1D shares approximately 50% amino acid homology, overall and in the carbohydrate recognition domain, with rat NKR-P1A and mouse NKR-P1A -B, and -C. Transcripts for NKR-P1A (1.1 kb) and NKR-P1D (2.0 kb) were produced by NK cells and NKR-P1dim/TCR alpha beta + T cells. Some NK cell clones coexpressed NKR-P1A and NKR-P1D. However, other clones lacking NKR-P1A produced message for NKR-P1D.
authors.affiliation: University of Pittsburgh Cancer Institute, Pa., USA.
authors.forename: P M
authors.initials: PM
authors.lastname: Appasamy
authors.forename: T W
authors.initials: TW
authors.lastname: Kenniston
authors.forename: C S
authors.initials: CS
authors.lastname: Brissette-Storkus
authors.forename: W H
authors.initials: WH
authors.lastname: Chambers
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D000954:Antigens, Surface / Q000096:biosynthesis* / Q000235:genetics; D001483:Base Sequence; D002469:Cell Separation; D002478:Cells, Cultured; D018076:DNA, Complementary / Q000235:genetics; D007694:Killer Cells, Natural / Q000378:metabolism*; D037181:Lectins, C-Type; D051379:Mice; D008969:Molecular Sequence Data; D055651:NK Cell Lectin-Like Receptor Subfamily B; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D051381:Rats; D011916:Rats, Inbred F344; D016693:Receptors, Antigen, T-Cell, alpha-beta / Q000032:analysis*; D016415:Sequence Alignment; D017385:Sequence Homology; D016176:T-Lymphocyte Subsets / Q000378:metabolism*
chemical_list: D000954:Antigens, Surface; D018076:DNA, Complementary; C527212:Klrb1b protein, mouse; D037181:Lectins, C-Type; D055651:NK Cell Lectin-Like Receptor Subfamily B; D012333:RNA, Messenger; D016693:Receptors, Antigen, T-Cell, alpha-beta