Detection of apolipoprotein E/dimeric soluble amyloid beta complexes in Alzheimer's disease brain supernatants.
The inheritance of the apolipoprotein (apo) E4 allele is an important risk factor for late-onset Alzheimer's disease (AD). A major component of the Alzheimer's disease neuritic plaques is amyloid beta (A beta). We previously identified apoE/A beta complexes within neuritic plaques (1). It was not known if this interaction takes place before or after A beta peptides become incorporated into neuritic plaques. To address this question we sought evidence of apoE complexes with brain soluble A beta peptides in AD and control patients. In addition, numerous proteins have been shown to bind A beta peptides in vitro. It is not know if any of these bind brain sA beta in vivo. We found evidence for the presence of apoE/dimeric sA beta complexes in the AD brain and could not detect complexes with other A beta peptide binding proteins. The binding of sA beta to apoE may be one factor influencing its clearance from the brain and/or its conformational state.
Permanne
B
B
Department of Pathology, New York University Medical Center, New York 10016, USA.
Perez
C
C
Soto
C
C
Frangione
B
B
Wisniewski
T
T
D000544:Alzheimer Disease / Q000378:metabolism*; D016229:Amyloid beta-Peptides / Q000032:analysis / Q000737:chemistry / Q000378:metabolism*; D001057:Apolipoproteins E / Q000032:analysis / Q000737:chemistry / Q000378:metabolism*; D015153:Blotting, Western; D001921:Brain / Q000378:metabolism*; D019281:Dimerization; D006239:Haplotypes; D006801:Humans; D013058:Mass Spectrometry; D011233:Precipitin Tests; D011485:Protein Binding; D011487:Protein Conformation; D012995:Solubility
D016229:Amyloid beta-Peptides; D001057:Apolipoproteins E