Growth inhibition of a human lung adenocarcinoma cell line by genetic complementation with chromosome 11.
Two regions on chromosome segment 11p15.5 have frequent allele loss in lung cancer. LOH11A is centromeric between loci D11S1758 and D11S12, and LOH11B is telomeric between HRAS and D11S1363. We studied the biological significance of this allele loss using microcell-mediated transfer of human chromosomes 11, 11p, and two radiation-reduced fragments of 11p into human lung adenocarcinoma cell lines. Chromosome 12, which has not been implicated in lung carcinogenesis, was used as a control. All four chromosome 11-containing hybrid clones showed significantly reduced tumorigenicity in nude mice and growth in liquid culture. These findings support the notion of a tumor suppressor gene located in the LOH11A region on chromosome segment 11p15.5.
O'Briant
K
K
Department of Medicine and Radiology, Duke University Medical Center, Durham, NC 27710, USA.
Jolicoeur
E
E
Garst
J
J
Campa
M
M
Schreiber
G
G
Bepler
G
G
D000230:Adenocarcinoma / Q000235:genetics* / Q000473:pathology; D000483:Alleles; D000818:Animals; D002455:Cell Division; D002880:Chromosomes, Human, Pair 11; D018014:Gene Transfer Techniques; D016147:Genes, Tumor Suppressor; D005816:Genetic Complementation Test; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D019656:Loss of Heterozygosity; D008175:Lung Neoplasms / Q000235:genetics* / Q000473:pathology; D051379:Mice; D008819:Mice, Nude; D009368:Neoplasm Transplantation; D014183:Transplantation, Heterologous