title: Regional CNS densities of serotonin and dopamine receptors in high alcohol-drinking (HAD) and low alcohol-drinking (LAD) rats.
abstract: The densities of subtypes of serotonin (5-HT) and dopamine (DA) receptors were determined in the CNS of male alcohol-naive HAD and LAD lines of rats. Autoradiographic studies were undertaken to measure the densities of (a) 5-HT1A sites labelled with 2 nM [3H]8-OH DPAT, (b) 5-HT2A sites labelled with 2 nM [3H] ketanserin, (c) D1 sites labelled with 1 nM [3H]SCH23390, and (d) D2 sites labelled with 20 nM [3H]sulpiride. Membrane binding, using tissue combined from the olfactory bulb, olfactory tubercle, and nucleus accumbens, was carried out to determine Kd and Bmax values for the binding of 0.25-8.0 nM [3H]7-OH DPAT to D3 sites. Among the 14 regions measured for densities of 5-HT1A sites, no interline differences were found in the cerebral cortical regions or in the septal nuclei; however, within the hippocampus, 15-20% lower binding of [3H]8-OH DPAT was observed in the posterior dorsal CA3 and dentate gyrus of the HAD line. There were no interline differences in any of the 10 regions examined for [3H]ketanserin binding to 5-HT2A sites, or in the densities of D1 and D2 sites in the mesolimbic and nigrostriatal DA systems, except for a 35% higher density of D2 sites in the substantia nigra pars compacta of the HAD line. There were no interline differences in the Kd or Bmax values for [3H]7-OH DPAT binding to D3 sites. Overall, these results indicate that no marked interline differences are evident in the densities of 5-HT1A, 5-HT2A, D1, D2, and D3 receptors within the mesolimbic system that could be associated with the disparate alcohol drinking behaviors of the HAD and LAD rats.
authors.affiliation: Department of Psychiatry, Indiana University School of Medicine, Indianapolis, USA.
authors.forename: W J
authors.initials: WJ
authors.lastname: McBride
authors.forename: E
authors.initials: E
authors.lastname: Chernet
authors.forename: R N
authors.initials: RN
authors.lastname: Russell
authors.forename: J K
authors.initials: JK
authors.lastname: Chamberlain
authors.forename: L
authors.initials: L
authors.lastname: Lumeng
authors.forename: T K
authors.initials: TK
authors.lastname: Li
mesh_terms: D017371:8-Hydroxy-2-(di-n-propylamino)tetralin / Q000378:metabolism; D000428:Alcohol Drinking; D000818:Animals; D001921:Brain / Q000378:metabolism*; D002421:Caudate Nucleus / Q000378:metabolism; D002540:Cerebral Cortex / Q000378:metabolism; D000431:Ethanol / Q000008:administration & dosage; D006624:Hippocampus / Q000378:metabolism; D007650:Ketanserin / Q000378:metabolism; D008297:Male; D009714:Nucleus Accumbens / Q000378:metabolism; D009833:Olfactory Pathways / Q000378:metabolism; D011699:Putamen / Q000378:metabolism; D051381:Rats; D011954:Receptors, Dopamine / Q000378:metabolism*; D011985:Receptors, Serotonin / Q000378:metabolism*; D013378:Substantia Nigra / Q000378:metabolism; D014316:Tritium
chemical_list: D011954:Receptors, Dopamine; D011985:Receptors, Serotonin; D014316:Tritium; D000431:Ethanol; D017371:8-Hydroxy-2-(di-n-propylamino)tetralin; D007650:Ketanserin