Detection of mineral-dust-induced DNA damage in two mammalian cell lines using the alkaline single cell gel/comet assay.
It has been estimated that over three million workers in the USA are potentially exposed to silica or other mineral dusts. Results of epidemiological studies evaluating whether silica or glass fibers increase lung cancer risk to the exposed workers are inconclusive. Detection of DNA damage in cells exposed to genotoxic agents is being used to assess the carcinogenic potential of environmental agents. The alkaline (pH > 13) single cell gel/comet (SCG) assay was used to determine and compare DNA damage in cultured Chinese hamster lung fibroblasts (V79 cells) and human embryonic lung fibroblasts (Hel 299 cells) exposed to crystalline silica (Min-U-Sil 5), amorphous silica (Spherisorb), carbon black, and glass fibers (AAA-10). V79 or Hel 299 cells were exposed to these mineral dusts for 3 h at various concentrations. Min-U-Sil 5 and AAA-10, at almost all concentrations tested, caused a significant increase in DNA migration measured as tail length in both V79 and Hel 299 exposed cells. However, the increase was much higher in V79 then in Hel 299 cells for Min-U-Sil 5. Tail length was also increased relative to controls after amorphous silica treatment, but not to the same extent as that induced by crystalline silica. Exposure to carbon black did not induce DNA migration at any of the concentrations tested. These results indicate that silica and glass fibers, but not carbon black, can induce DNA damage in mammalian cells, and that crystalline silica has a higher DNA-damaging activity than amorphous silica. For glass fibers, induction of DNA damage in both V79 and Hel 299 cells was observed even at a concentration 10 times lower than silica and the response was similar in both cell lines. These results suggest that the SCG/comet assay is useful for the detection of DNA damage caused by occupationally related dusts/particles.
Zhong
B Z
BZ
National Institute for Occupational Safety and Health, Health Effects Laboratory Division, Morgantown, WV 26505-2888, USA.
Whong
W Z
WZ
Ong
T M
TM
D000818:Animals; D002244:Carbon / Q000633:toxicity*; D002460:Cell Line; D006224:Cricetinae; D004247:DNA / Q000032:analysis; D004249:DNA Damage / Q000187:drug effects*; D004260:DNA Repair / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D004587:Electrophoresis, Agar Gel / Q000379:methods; D005347:Fibroblasts / Q000187:drug effects; D005898:Glass; D006801:Humans; D008168:Lung / Q000166:cytology / Q000187:drug effects*; D008647:Mesocricetus; D012821:Silanes / Q000633:toxicity*; D012822:Silicon Dioxide / Q000633:toxicity*
D012821:Silanes; C009753:fiberglass; C047147:spherisorb octadecylsilane; D002244:Carbon; D012822:Silicon Dioxide; D004247:DNA