Inhibition of potassium dichromate mutagenicity by todralazine.
Todralazine, an antihypertensive drug of the hydrazinoph-thalazine group, markedly decreased the mutagenic activity of potassium dichromate in standard bacterial tests. At the highest todralazine dose tested inhibition of potassium dichromate mutagenic activity by approximately 90% in the Ames test and up to 100% (complete) inhibition in the Bacillus subtilis rec- assay was observed. Spectrophotometric analyses proved that todralazine induced reduction of Cr(VI) to Cr(III) and complexation of Cr(III) ions. These spectro-photometric results may be a presumptive explanation of the observed mutagenic activity decrease, as it is known that Cr(III) is poorly transported across cell membranes and therefore is not mutagenic to bacterial cells. We perceive our experiments as an example of attempts which should lead to an effective reduction in chromium genotoxic and carcinogenic activity in exposed individuals.
Gasiorowski
K
K
Medical University, Department of Basic Medical Sciences, Wroclaw, Poland.
Szyba
K
K
Wozniak
D
D
Gulanowski
B
B
D000959:Antihypertensive Agents / Q000494:pharmacology*; D016587:Antimutagenic Agents / Q000494:pharmacology; D001412:Bacillus subtilis / Q000187:drug effects; D002424:Caustics / Q000633:toxicity*; D004305:Dose-Response Relationship, Drug; D016296:Mutagenesis / Q000187:drug effects; D009152:Mutagenicity Tests; D009153:Mutagens / Q000633:toxicity*; D011192:Potassium Dichromate / Q000037:antagonists & inhibitors* / Q000737:chemistry / Q000633:toxicity*; D012044:Regression Analysis; D012486:Salmonella typhimurium / Q000187:drug effects; D013053:Spectrophotometry; D014032:Todralazine / Q000737:chemistry / Q000494:pharmacology*
D000959:Antihypertensive Agents; D016587:Antimutagenic Agents; D002424:Caustics; D009153:Mutagens; D011192:Potassium Dichromate; D014032:Todralazine