A new generation of serum anti-HIV antibody immunocapture assay for saliva testing.
BACKGROUND
Epidemiological studies need easier tools to evaluate HIV prevalence, particularly in high-risk groups under difficult field conditions. Testing saliva antibody with accurate immunoassay could serve as an alternative to serum testing.


OBJECTIVE
To evaluate performances on saliva of a novel commercially available assay for anti-HIV antibody.


METHODS
Samples of saliva from 530 patients were tested for the presence of HIV antibodies with a third generation commercially available serum-screening kit and the use of a sample diluent adapted to saliva.


RESULTS
Compared with serum sampling the sensitivity and specificity of oral sampling were respectively, 100 and 99.8%.


CONCLUSIONS
The ICE HIV-1.0.2 assay used on saliva could be an efficient and non-invasive epidemiological tool for HIV testing.
Pasquier
C
C
Laboratoire Universitaire des Rétrovirus, Hôpital Purpan, Toulouse, France.
Bello
P Y
PY
Gourney
P
P
Puel
J
J
Izopet
J
J
D000328:Adult; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D005069:Evaluation Studies as Topic; D015483:HIV Antibodies / Q000032:analysis*; D015658:HIV Infections / Q000175:diagnosis*; D006801:Humans; D011933:Reagent Kits, Diagnostic; D012463:Saliva / Q000821:virology*; D012680:Sensitivity and Specificity
D015483:HIV Antibodies; D011933:Reagent Kits, Diagnostic