Changes in anion permeability following hypotonic challenge in rat brain endothelial cells: different responses in primary cultures and in immortalised RBE4 cells.
Hypotonicity-induced anion permeability changes were investigated but not detected in immortalised (RBE4) rat brain endothelial cells using iodide efflux measurements. Large, rapid increases were however observed in primary cultured cells. Both cell types were reinvestigated following culture in a common growth factor-depleted medium. Responses were still undetectable in the immortalised RBE4 cells. Reduced responses were observed in the primary cultured cells that also showed altered morphology and decreased activity of another transporter, P-glycoprotein. Thus both immortalisation and different culture conditions may alter functional expression in these cells of transporters involved in hypotonicity-induced anion permeability changes.
von Weikersthal
S F
SF
Department of Pharmacology, University of Cambridge, UK.
Seetharaman
S
S
Wright
K A
KA
Roux
F A
FA
Barrand
M A
MA
D020168:ATP Binding Cassette Transporter, Subfamily B, Member 1 / Q000187:drug effects / Q000378:metabolism*; D000818:Animals; D000838:Anions / Q000378:metabolism; D002461:Cell Line, Transformed; D002463:Cell Membrane Permeability / Q000502:physiology*; D002540:Cerebral Cortex / Q000098:blood supply*; D016572:Cyclosporine / Q000494:pharmacology; D004730:Endothelium, Vascular / Q000166:cytology / Q000502:physiology*; D007038:Hypotonic Solutions; D007454:Iodides / Q000378:metabolism*; D007700:Kinetics; D008833:Microcirculation; D051381:Rats; D014700:Verapamil / Q000494:pharmacology; D014750:Vincristine / Q000493:pharmacokinetics
D020168:ATP Binding Cassette Transporter, Subfamily B, Member 1; D000838:Anions; D007038:Hypotonic Solutions; D007454:Iodides; D014750:Vincristine; D016572:Cyclosporine; D014700:Verapamil