Inhibitory effect on curcumin on mammalian phospholipase D activity.
Curcumin, the major yellow pigment of turmeric (Curcuma longa), has strong anti-carcinogenic and anti-inflammatory activities. We examined the effects of curcumin on enzyme activities of the following phospholipases in a cell-free system: G protein-mediated phospholipase D (PLD), phosphatidylinositol-specific phospholipase C, and phospholipase A2 from mouse macrophage-like cell line J774.1 cells, sphingomyelinase from bovine brain, and phosphatidylcholine-phospholipase C from Bacillus cereus. Curcumin inhibited several types of phospholipases, most effectively PLD among those tested. It also inhibited 12-O-tetradecanoylphorbol-13-acetate-induced PLD activation in intact J774.1 cells in a dose-dependent manner. These results suggest that the anti-inflammatory and anti-carcinogenic action of curcumin is partly due to the inhibition of PLD.
Yamamoto
H
H
Department of Biochemistry and Cell Biology, National Institute of Infectious Diseases, Toyama, Tokyo, Japan.
Hanada
K
K
Kawasaki
K
K
Nishijima
M
M
D000818:Animals; D001412:Bacillus subtilis / Q000201:enzymology; D001426:Bacterial Proteins; D002417:Cattle; D002474:Cell-Free System; D002478:Cells, Cultured; D003474:Curcumin / Q000494:pharmacology*; D004789:Enzyme Activation / Q000187:drug effects; D004791:Enzyme Inhibitors / Q000494:pharmacology; D051379:Mice; D010739:Phospholipase D / Q000037:antagonists & inhibitors*; D010741:Phospholipases A / Q000378:metabolism; D054467:Phospholipases A2; D013108:Sphingomyelin Phosphodiesterase / Q000378:metabolism; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology; D010738:Type C Phospholipases / Q000378:metabolism
D001426:Bacterial Proteins; D004791:Enzyme Inhibitors; D010741:Phospholipases A; D054467:Phospholipases A2; D010738:Type C Phospholipases; D013108:Sphingomyelin Phosphodiesterase; D010739:Phospholipase D; D003474:Curcumin; D013755:Tetradecanoylphorbol Acetate