Evidence that exposure of particulate air pollutants to human and rat alveolar macrophages leads to differential oxidative response.
Macrophages and inflammatory cells generate active oxygen species in the process of killing and degrading microorganisms. Air pollutant particles may be ingested by macrophages and stimulate the same mechanisms to produce a long term oxidative burden to the lung if particles are not degraded. In the present study human and rat alveolar macrophages (AM) were compared in their response to inhaled particles using luminol dependent chemiluminescence (CL) and peroxide dependent CL assays. Cytotoxicity was measured by the release of lactate dehydrogenase (LDH) activity in the supernatant. Human AM produced more oxidants than rat AM whether, unstimulated, after addition of particles or addition of particles then peroxidase. Human AM also had a different spectrum of response to the same particles. Our results suggest that human macrophages produce more reactive oxygen species in respond to particles than rat AM.
Rahman
Q
Q
Industrial Toxicology Research Centre, Lucknow, India.
Norwood
J
J
Hatch
G
G
D000293:Adolescent; D000328:Adult; D000393:Air Pollutants / Q000494:pharmacology*; D000818:Animals; D006735:Horseradish Peroxidase / Q000378:metabolism; D006801:Humans; D066298:In Vitro Techniques; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D008163:Luminescent Measurements; D008165:Luminol / Q000378:metabolism; D016676:Macrophages, Alveolar / Q000378:metabolism*; D008297:Male; D018384:Oxidative Stress; D051381:Rats; D017207:Rats, Sprague-Dawley; D017382:Reactive Oxygen Species / Q000378:metabolism*
D000393:Air Pollutants; D017382:Reactive Oxygen Species; D008165:Luminol; D007770:L-Lactate Dehydrogenase; D006735:Horseradish Peroxidase