title: Oligoclonal expansion of muscle infiltrating T cells in inclusion body myositis.
abstract: Inclusion body myositis (IBM) is the most common muscle disease affecting individuals over 50 years of age. An important feature of IBM is invasion of muscle fibers by T cells. The muscle infiltrating T cells show a restricted usage of variable (V) alpha/beta gene families. In this study we have investigated the clonality of T cells using two of the predominant V beta families i.e. V beta 3 and V beta 8 in three patients with IBM. The study was performed by reverse transcription and polymerase chain reaction (RT-PCR) analysis, followed by cloning and sequencing of the T cell receptor complementarity determining region 3. We found oligoclonal expansion of V beta 3 bearing muscle infiltrating T cells in two patients and of V beta 8 in one patient, supporting the concept that antigen stimulated T cells are important in the pathogenesis of IBM. Results of HLA typing indicated a genetic predisposition for the disease by the presence of DR3, DR52 and DQB1*0201/0202 in all three patients.
authors.affiliation: Department of Pathology, Sahlgrenska Hospital, Gothenburg University, Sweden.
authors.forename: I M
authors.initials: IM
authors.lastname: Fyhr
authors.forename: A R
authors.initials: AR
authors.lastname: Moslemi
authors.forename: A A
authors.initials: AA
authors.lastname: Mosavi
authors.forename: C
authors.initials: C
authors.lastname: Lindberg
authors.forename: A
authors.initials: A
authors.lastname: Tarkowski
authors.forename: A
authors.initials: A
authors.lastname: Oldfors
mesh_terms: D000368:Aged; D000595:Amino Acid Sequence; D003001:Cloning, Molecular; D005260:Female; D006801:Humans; D007135:Immunoglobulin Variable Region / Q000235:genetics; D007150:Immunohistochemistry; D008297:Male; D008875:Middle Aged; D008969:Molecular Sequence Data; D005810:Multigene Family; D009132:Muscles / Q000473:pathology*; D018979:Myositis, Inclusion Body / Q000235:genetics / Q000473:pathology*; D016133:Polymerase Chain Reaction; D016693:Receptors, Antigen, T-Cell, alpha-beta / Q000235:genetics / Q000378:metabolism; D013601:T-Lymphocytes / Q000378:metabolism / Q000473:pathology*; D014158:Transcription, Genetic
chemical_list: D007135:Immunoglobulin Variable Region; D016693:Receptors, Antigen, T-Cell, alpha-beta