Modulation of TCR usage in HIV-1 infection is regulated by IL-7 and sCD23.
The purpose of this study is to elucidate the effect of interleukin-7 (IL-7) and soluble CD23 (sCD23) on Phorbol12 Myristate13 Acetate (PMA) activated CD4+ TCR alpha beta+ cells of HIV-1 infected subjects. CD23 and IL-7R were detectable on activated CD4+ T cells of these subjects by FACS. Addition on IL-7 (1000 U/ml), at the onset of cultures, resulted in a significant increase of CD23 expression. We also demonstrated that T cells proliferation and CD23 expression in the presence of exogenous IL-7 occur in an IL-2 independent manner. Addition of IL-7 and sCD23 to activated CD4+ cells of HIV-1 infected subjects induced a proliferative response of TCR alpha beta cells. In contrast, addition of either sCD23 or IL-7 to activated CD4+ T cells did not result in an increase of TCR alpha beta expression. The data provide direct evidence that sCD23 in combination with IL-7 induce proliferation of activated CD4+ T cells of HIV-1 infected subjects to augment TCR alpha beta expression. These results support the possibility that IL-7 plus sCD23 might play an important role in the modulation of TCR alpha beta expression in HIV infection.
Carini
C
C
Department of Cancer Biology, Harvard School of Public Health, Harvard Medical School, Boston, MA, USA.
Guerriero
M
M
Fratazzi
C
C
D000328:Adult; D015703:Antigens, CD / Q000378:metabolism; D015496:CD4-Positive T-Lymphocytes / Q000187:drug effects / Q000276:immunology; D018414:CD8-Positive T-Lymphocytes / Q000187:drug effects / Q000276:immunology; D015658:HIV Infections / Q000276:immunology*; D006801:Humans; D015851:Interleukin-7 / Q000494:pharmacology*; D008213:Lymphocyte Activation; D008297:Male; D016693:Receptors, Antigen, T-Cell, alpha-beta / Q000187:drug effects*; D017455:Receptors, IgE / Q000378:metabolism*; D018123:Receptors, Interleukin / Q000378:metabolism; D020395:Receptors, Interleukin-7; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology
D015703:Antigens, CD; D015851:Interleukin-7; D016693:Receptors, Antigen, T-Cell, alpha-beta; D017455:Receptors, IgE; D018123:Receptors, Interleukin; D020395:Receptors, Interleukin-7; D013755:Tetradecanoylphorbol Acetate