[Determination of uric acid in scalp hair for non-invasive estimation of uricemic control in hyperuricemia].
Uric acid in blood has been widely accepted as a reliable indicator of hyperuricemia and gout, and its assay method has been established. In the present study, we developed a simple and non-invasive rapid method for the determination of uric acid in hair. Concentration(nmol/mg hair) of uric acid extracted from 10-20 mg of hair(95% < extractability; 0.1N KOH, 37 degrees C, 2 hr) was determined by an enzymatic method using uricase. The concentration of uric acid(nmol/mg hair, mean +/- SD: 0.489 +/- 0.157, n = 16) in hair from hyperuricemic patients was significantly higher than that(0.258 +/- 0.107, n = 8) from healthy volunteers(p < 0.01). Within-run and between-day precisions(reproducibilities, CVs) for the assay were 9.6-10.3%(n = 10 each) and 11.6-16.3%(n = 7 each), respectively. The concentration(y, nmol/mg hair) of uric acid in hair correlated well with that in blood(x, g/l): y = 8.770x-0.123(r = 0.746, Syx = 0.122, n = 23). Changes in the concentration of uric acid in hair of hyperuricemic patient treated with allopurinol paralleled to those in blood. In conclusion, it was confirmed that the concentration of uric acid in hair reflected that in blood, suggesting that measuring uric acid in hair can be available for the metabolic control in hyperuricemia.
Kobayashi
K
K
Diagnostic Science Division, Shionogi & Co., Ltd., Settsu.
Morioka
Y
Y
Isaka
Y
Y
Tozawa
T
T
D015415:Biomarkers / Q000032:analysis; D006073:Gout / Q000175:diagnosis / Q000378:metabolism*; D006197:Hair / Q000737:chemistry*; D006801:Humans; D012680:Sensitivity and Specificity; D014527:Uric Acid / Q000032:analysis*
D015415:Biomarkers; D014527:Uric Acid