Reversed-phase HPLC determination of eight anticoagulant rodenticides in animal liver.
A reversed-phase high-performance liquid chromatographic method was developed for the analysis of eight anticoagulant rodenticides in animal liver. Coumarinic anticoagulant rodenticides (brodifacoum, bromadiolone, coumachlor, coumatetralyl, difenacoum, and warfarin) were detected by using a gradient elution and a fluorimetric detection. Indanedione anticoagulant rodenticides (chlorophacinone and diphacinone) were detected by using an isocratic elution and an UV detection. Anticoagulants were extracted from liver with mixtures of acetone/diethylether and acetone/chloroform. Extracts were applied to solid-phase extraction cartridges. Linearity was checked over the concentration range 0.1-0.6 microgram/g. Relative standard deviations of within-run and between-run variability were all between 5.7 and 10.3%. Recoveries from spiked liver samples were between 51.7 (difenacoum) and 78.2% (warfarin). Limits of detection were between 0.01 (difenacoum and warfarin) and 0.11 microgram/g (chlorophacinone).
Fauconnet
V
V
Ecole Nationale Vétérinaire de Nantes, Laboratoire de Pharmacie et Toxicologie, Nantes, France.
Pouliquen
H
H
Pinault
L
L
D000818:Animals; D000925:Anticoagulants / Q000032:analysis*; D002138:Calibration; D002851:Chromatography, High Pressure Liquid; D003374:Coumarins / Q000032:analysis; D008099:Liver / Q000737:chemistry*; D051381:Rats; D012378:Rodenticides / Q000032:analysis*; D012997:Solvents; D013050:Spectrometry, Fluorescence; D013056:Spectrophotometry, Ultraviolet
D000925:Anticoagulants; D003374:Coumarins; D012378:Rodenticides; D012997:Solvents