title: Confirmation of malachite green, gentian violet and their leuco analogs in catfish and trout tissue by high-performance liquid chromatography utilizing electrochemistry with ultraviolet-visible diode array detection and fluorescence detection.
abstract: A sensitive analytical procedure for the confirmation of residues of malachite green (MG), gentian violet (GV) and their leuco analogs (LMG and LGV) in catfish and trout tissue at 10 ng/g is described. Frozen (-20 degrees C) fish fillets were cut into small pieces and homogenized in Waring blendors. The compounds of interest were extracted from 20-g amounts of homogenized fish tissue with acetonitrile-buffer, partitioned against methylene chloride, and isolated with tandem neutral alumina and propylsulfonic acid cation-exchange solid-phase extraction cartridges. Samples of 100 microl (0.8 g equiv.) were chromatographed isocratically in 10 min using an acetonitrile-buffer mobile phase on a short-chain deactivated (SCD) reversed-phase column (150x4.6 mm I.D.) in-line with a post-column oxidation coulometric electrochemical cell (EC), a UV-Vis diode array detector and a fluorescence detector.
authors.affiliation: Department of Health & Human Services, Public Health Service, Food and Drug Administration, National Center for Toxicological Research, Jefferson, AR 72079-9502, USA.
authors.forename: L G
authors.initials: LG
authors.lastname: Rushing
authors.forename: E B
authors.initials: EB
authors.lastname: Hansen
mesh_terms: D000818:Animals; D000935:Antifungal Agents / Q000032:analysis* / Q000737:chemistry; D002397:Catfishes / Q000378:metabolism*; D002851:Chromatography, High Pressure Liquid; D004350:Drug Residues / Q000032:analysis*; D004563:Electrochemistry; D005840:Gentian Violet / Q000032:analysis* / Q000737:chemistry; D010084:Oxidation-Reduction; D012394:Rosaniline Dyes / Q000032:analysis* / Q000737:chemistry; D013050:Spectrometry, Fluorescence; D013056:Spectrophotometry, Ultraviolet; D014337:Trout / Q000378:metabolism*
chemical_list: D000935:Antifungal Agents; D012394:Rosaniline Dyes; C005095:malachite green; D005840:Gentian Violet