title: Crystallization and preliminary X-Ray diffraction characterization of a dimerizing fragment of the rod domain of the Dictyostelium gelation factor (ABP-120).
abstract: We have expressed in Escherichia coli a construct corresponding to sequence repeats 5 and 6 of the rod domain of the actin-binding protein Dictyostelium gelation factor (ABP-120). We have obtained orthorhombic P212121 crystals of the protein with a = 43.5 A, b = 103.2 A, c = 124.4 A. These crystals diffract past 2.2 A resolution using synchrotron radiation and are suitable for high-resolution structural analysis. ABP-120 is a key component of the Dictyostelium cytoskeleton, where it functions to crosslink F-actin filaments into networks. This crosslinking function of ABP-120 depends crucially on the formation of dimeric molecules that contain an actin-binding site on each chain, and this dimerization is brought about through interactions between repeating sequence modules in the rod domain. Because the construct we have expressed retains the ability to dimerize, it should enable us to establish the precise manner in which these sequence repeats interact with one another in the intact molecule.
authors.affiliation: Max-Planck-Institut für Biochemie, Hills Road, Cambridge, CB2 2QH, England.
authors.forename: P
authors.initials: P
authors.lastname: Fucini
authors.forename: A J
authors.initials: AJ
authors.lastname: McCoy
authors.forename: M
authors.initials: M
authors.lastname: Gomez-Ortiz
authors.forename: M
authors.initials: M
authors.lastname: Schleicher
authors.forename: A A
authors.initials: AA
authors.lastname: Noegel
authors.forename: M
authors.initials: M
authors.lastname: Stewart
mesh_terms: D000818:Animals; D002352:Carrier Proteins / Q000737:chemistry*; D003460:Crystallization; D018360:Crystallography, X-Ray; D004023:Dictyostelium / Q000737:chemistry*; D019281:Dimerization; D004926:Escherichia coli / Q000235:genetics; D008840:Microfilament Proteins / Q000737:chemistry* / Q000235:genetics; D008859:Microscopy, Polarization; D010446:Peptide Fragments / Q000737:chemistry / Q000235:genetics; D015202:Protein Engineering; D011994:Recombinant Proteins / Q000737:chemistry / Q000302:isolation & purification
chemical_list: D002352:Carrier Proteins; D008840:Microfilament Proteins; D010446:Peptide Fragments; D011994:Recombinant Proteins; C060680:abpC protein, Dictyostelium