Panton-valentine leukocidin genes in a phage-like particle isolated from mitomycin C-treated Staphylococcus aureus V8 (ATCC 49775).
The staphylococcal Panton-Valentine leukocidin (PVL) genes [lukS-PV-lukF-PV] existed in a hexagonal phage-like particle (phi PVL) isolated from mitomycin C-induced Staphylococcus aureus V8 (ATCC 49775). The genome packed in phi PVL was a linear double-stranded 40-kb DNA with single-stranded cohesive ends (cos). The [lukS-PV-lukS-PV], attP, and int (integrase gene) of phi PVL were all located very close to one another within a 4.0 kb-segment on the genome in the order given, and the segment is located at the center from the left and the right cos sites. In addition, the [lukS-PV-lukF-PV]-attP-int region contains 5 direct repeat sequences that show high similarity with the recombinase-binding sites of bacteriophages of S. aureus.
Kaneko
J
J
Department of Applied Biological Chemistry, Faculty of Agriculture, Tohoku University, Sendai, Japan.
Kimura
T
T
Kawakami
Y
Y
Tomita
T
T
Kamio
Y
Y
D000595:Amino Acid Sequence; D001435:Bacteriophages / Q000235:genetics*; D001483:Base Sequence; D001665:Binding Sites; D004269:DNA, Bacterial / Q000235:genetics / Q000378:metabolism; D005798:Genes, Bacterial; D007956:Leukocidins / Q000235:genetics*; D016685:Mitomycin / Q000494:pharmacology*; D008969:Molecular Sequence Data; D019384:Nucleic Acid Synthesis Inhibitors / Q000494:pharmacology*; D012091:Repetitive Sequences, Nucleic Acid; D017386:Sequence Homology, Amino Acid; D013211:Staphylococcus aureus / Q000187:drug effects* / Q000235:genetics* / Q000821:virology
D004269:DNA, Bacterial; D007956:Leukocidins; D019384:Nucleic Acid Synthesis Inhibitors; D016685:Mitomycin