Amplification of rfbE and fliC genes by polymerase chain reaction for identification and detection of Salmonella serovar Enteritidis, Dublin and Gallinarum-Pullorum.
Polymerase chain reaction (PCR) primers for O9 antigen (rfbE) and phase 1 flagellin antigen (fliC) were designed for the rapid identification and detection of Salmonella serovar Enteritidis and Dublin. The rfbE primer pairs selectively amplified the rfbE region of group O9 Salmonella serovars. The fliC primer pairs amplified the DNAs of g,m and g,p-type flagellar antigen; Salmonella serovar Enteritidis, Dublin, and Essen. However, DNA from flagellar-negative Salmonella serovar Gallinarum-Pullorum was also amplified. The sensitivity of PCR primer pairs was 10(4) CFU/assay by boiled DNA preparation and 10(2) CFU/assay by proteinase K-treated DNA preparation.
Itoh
Y
Y
Department of Microbiology, Gifu University School of Medicine, Japan.
Hirose
K
K
Miyake
M
M
Khan
A Q
AQ
Hashimoto
Y
Y
Ezaki
T
T
D002238:Carbohydrate Epimerases / Q000235:genetics*; D017931:DNA Primers; D004269:DNA, Bacterial / Q000032:analysis; D004587:Electrophoresis, Agar Gel; D005408:Flagellin / Q000235:genetics*; D005798:Genes, Bacterial; D016133:Polymerase Chain Reaction; D012475:Salmonella / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D012477:Salmonella enteritidis / Q000235:genetics / Q000302:isolation & purification*; D012680:Sensitivity and Specificity; D012703:Serotyping; D000637:Transaminases / Q000235:genetics*
D017931:DNA Primers; D004269:DNA, Bacterial; D005408:Flagellin; D000637:Transaminases; D002238:Carbohydrate Epimerases; C061272:perosamine synthetase