title: Production of Escherichia coli group I-like heat-labile enterotoxin by Enterobacteriaceae isolated from environmental water.
abstract: Strains of Klebsiella oxytoca (2), Citrobacter freundii (2), Enterobacter cloacae (1) and Escherichia coli (1) isolated from environmental water were identified as heat-labile enterotoxin (LT) producing strains by immunological methods and polymerase chain amplification. A 322 bp amplified fragment was obtained with specific primers LTR and LTL, and hybridized to a digoxigenin-labelled LTB probe only under low stringency conditions, and not with a cholera toxin probe. These results suggest that Enterobacteriaceae may produce a LT-like toxin antigenically and genetically related to the LT enterotoxin of E. coli.
authors.affiliation: Laboratoire de Microbiologie Pharmaceutique, Faculté de Sciences Pharmaceutiques et Biologiques, Université de Rennes 1, France.
authors.forename: A
authors.initials: A
authors.lastname: Jolivet-Gougeon
authors.forename: T
authors.initials: T
authors.lastname: Tamanai-Shacoori
authors.forename: F
authors.initials: F
authors.lastname: Sauvager
authors.forename: M
authors.initials: M
authors.lastname: Cormier
mesh_terms: D001427:Bacterial Toxins / Q000096:biosynthesis* / Q000235:genetics; D004755:Enterobacteriaceae / Q000472:pathogenicity*; D004768:Enterotoxins / Q000096:biosynthesis* / Q000235:genetics; D004926:Escherichia coli / Q000472:pathogenicity*; D029968:Escherichia coli Proteins; D016133:Polymerase Chain Reaction; D014871:Water Microbiology
chemical_list: D001427:Bacterial Toxins; D004768:Enterotoxins; D029968:Escherichia coli Proteins; C027538:heat-labile enterotoxin, E coli