title: Mutations at codon 249 of p53 gene in human hepatocellular carcinomas from Tongan, China.
abstract: Codon 249 (exon 7) of the putative tumor suppressor gene p53 is a mutational hot-spot for hepatocellular carcinoma (HCC) but not other tumors. DNA samples from primary HCC patients from Tongan, an area of high HCC incidence in China (> 40 per 100,000 population), were analyzed for specific mutations in codon 249 of the p53 gene using polymerase chain reaction (PCR)/restriction-digest methods and direct DNA sequencing. Seven of the 21 samples screened were found to have a point mutation at the third base position of codon 249 (AGG to AGT). The result is consistent with previous reports that the G-->T transversion is positively associated with the level of dietary aflatoxin B1 (AFB1) contamination, which has been implicated as one of the risk factors in Tongan area. Of the 7 HCC patients that contained the codon 249 point mutation, one was hepatitis B virus (HBV)-negative. This is only the second documentation of an HCC patient harboring the p53 codon 249 mutation, who was HBV-negative.
authors.affiliation: Department of Biology and Chemistry, City University of Hong Kong, Kowloon, Hong Kong. bhmyang@cityu.edu.hk
authors.forename: M
authors.initials: M
authors.lastname: Yang
authors.forename: H
authors.initials: H
authors.lastname: Zhou
authors.forename: R Y
authors.initials: RY
authors.lastname: Kong
authors.forename: W F
authors.initials: WF
authors.lastname: Fong
authors.forename: L Q
authors.initials: LQ
authors.lastname: Ren
authors.forename: X H
authors.initials: XH
authors.lastname: Liao
authors.forename: Y
authors.initials: Y
authors.lastname: Wang
authors.forename: W
authors.initials: W
authors.lastname: Zhuang
authors.forename: S
authors.initials: S
authors.lastname: Yang
mesh_terms: D000328:Adult; D006528:Carcinoma, Hepatocellular / Q000235:genetics*; D002681:China; D003062:Codon; D015252:Deoxyribonucleases, Type II Site-Specific / Q000378:metabolism; D005260:Female; D016158:Genes, p53; D006514:Hepatitis B Surface Antigens / Q000097:blood; D006801:Humans; D008113:Liver Neoplasms / Q000235:genetics*; D008297:Male; D008875:Middle Aged; D009154:Mutation; D016133:Polymerase Chain Reaction / Q000379:methods; D015183:Restriction Mapping; D012307:Risk Factors; D017422:Sequence Analysis, DNA; D000509:alpha-Fetoproteins / Q000032:analysis
chemical_list: D003062:Codon; D006514:Hepatitis B Surface Antigens; D000509:alpha-Fetoproteins; D015252:Deoxyribonucleases, Type II Site-Specific; C024403:GGCC-specific type II deoxyribonucleases