[The electrophoretic properties of gels of high-resolution MS-4 agarose for separating DNA fragments in molecular genetic analysis].
The Hispanlab firm (Spain) offers a new series of Molecular Screen analytical agarose, including MS-4 agarose intended for separation of small fragments of DNA. This low-endosmotic agarose attracts attention as a probable alternative to well-known FMC Bio Products special agarose (USA) due to its useful properties. We characterized a gel-electrophoretic system based on MS-4 agarose (and its correspondence to its passport) and tested its fitness for standard molecular genetic expert investigations. The following parameters were assessed: mechanical properties of gel, its transparency, degree of background fluorescence upon ethidium bromide staining, resolving power, and position distortions in the electrophoregram. Important features of MS-4 agarose gels were detected, not typical of other agarose systems, which should be borne in mind during investigations with this agarose.
Zaiats
M V
MV
Frolova
S A
SA
Ivanov
P L
PL
D004247:DNA / Q000302:isolation & purification*; D004587:Electrophoresis, Agar Gel / Q000379:methods* / Q000706:statistics & numerical data; D005069:Evaluation Studies as Topic; D005260:Female; D005782:Gels; D006801:Humans; D008297:Male; D016133:Polymerase Chain Reaction; D012044:Regression Analysis; D012685:Sepharose / Q000737:chemistry
D005782:Gels; D004247:DNA; D012685:Sepharose