title: Protein-protein interaction between the RVS161 and RVS167 gene products of Saccharomyces cerevisiae.
abstract: As previous studies indicated that the RVS161 and RVS167 gene products of Saccharomyces cerevisiae seem to be involved in the same cellular function, we considered the possibility of a complex between the proteins encoded by these two genes. Using the two hybrid system, we have shown that Rvs167p interacts with Rvs161p, through its N-terminal domain which contains predicted coiled-coil structures. Moreover, if a tagged Rvs 167p protein was immuno adsorbed under non-denaturing conditions, it brought along the Rvs161p protein. These results confirmed the hypothesis of an in vivo complex between the two proteins.
authors.affiliation: Laboratoire de Biologie Cellulaire des Levures, IBGC-CNRS, Bordeaux, France.
authors.forename: P
authors.initials: P
authors.lastname: Navarro
authors.forename: P
authors.initials: P
authors.lastname: Durrens
authors.forename: M
authors.initials: M
authors.lastname: Aigle
mesh_terms: D015153:Blotting, Western; D003598:Cytoskeletal Proteins; D019281:Dimerization; D005656:Fungal Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D017930:Genes, Reporter / Q000235:genetics; D008840:Microfilament Proteins; D010957:Plasmids / Q000235:genetics; D011233:Precipitin Tests; D011487:Protein Conformation; D017433:Protein Structure, Secondary; D012441:Saccharomyces cerevisiae / Q000235:genetics / Q000378:metabolism*; D029701:Saccharomyces cerevisiae Proteins; D001616:beta-Galactosidase / Q000235:genetics / Q000378:metabolism
chemical_list: D003598:Cytoskeletal Proteins; D005656:Fungal Proteins; D008840:Microfilament Proteins; C089181:RVS161 protein, S cerevisiae; C082282:RVS167 protein, S cerevisiae; D029701:Saccharomyces cerevisiae Proteins; D001616:beta-Galactosidase