[Effects of the nef and tat genes of the human immunodeficiency virus type I on rodent cells in vivo and in vitro].
The tat and nef regulatory genes of the human immunodeficiency virus type I (HIV-1) under the control of eukaryotic promoters were transferred in vivo into mice and in vitro into rat cell cultures. The development was disturbed and adenocarcinomas of the lacrimal glands and pancreas appeared in transgenic mice carrying the HIV-1 tat gene. Transfection with the tat gene altered morphology and increased proliferative activity of Rat-2 pseudonormal cells. The tat gene also induced the formation of neoplastic foci in a primary rat embryo fibroblast culture. The results obtained showed that the HIV-1 tat gene can act as an oncogene and activate the proliferation of cultured cells. Cell proportions in peripheral blood and bone marrow were altered and mitogen-induced lymphocyte proliferation was decreased in transgenic mice carrying the HIV-1 nef gene. This gene also significantly suppressed proliferation but had no effect on morphology of Rat-2 cells. Thus, the HIV-1 nef gene appeared to suppress proliferation of various animal cells.
Shugurova
I M
IM
Andreeva
L E
LE
Dubovaia
V I
VI
Zueva
L A
LA
Serova
I A
IA
Tarantul
V Z
VZ
D000818:Animals; D002455:Cell Division; D002472:Cell Transformation, Viral; D002478:Cells, Cultured; D005809:Genes, Regulator; D015754:Genes, nef; D015756:Genes, tat; D015497:HIV-1 / Q000235:genetics*; D051379:Mice; D008822:Mice, Transgenic; D051381:Rats