Maturation and translation mechanisms involved in the expression of a myb gene of rice.
We have isolated two overlapping cDNAs coding for a MYB-related protein expressed in aerobic and anaerobic rice (Oryza sativa) roots and coleoptiles. Analysis of their sequences reveals some peculiar features, suggesting the presence of post-transcriptional regulation events: an upstream ORF, two unspliced introns and a putative leucine zipper in the ORF coded by the unspliced RNA. Transient expression in protoplasts indicates that the upstream ORF inhibits expression of a downstream coding sequence. Finally, we demonstrated that anoxia, in roots, increases the ratio between the spliced and the unspliced mRNA and affects the expression of other myb-related genes.
Magaraggia
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F
Istituto Biosintesi Vegetali, C.N.R., Milano, Italy.
Solinas
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G
Valle
G
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Giovinazzo
G
G
Coraggio
I
I
D018507:Gene Expression Regulation, Developmental; D018506:Gene Expression Regulation, Plant; D008969:Molecular Sequence Data; D012275:Oryza / Q000235:genetics* / Q000254:growth & development; D010940:Plant Proteins / Q000096:biosynthesis / Q000235:genetics* / Q000502:physiology; D014176:Protein Biosynthesis; D014157:Transcription Factors / Q000096:biosynthesis / Q000235:genetics* / Q000502:physiology
C106119:OsGAmyb protein, Oryza sativa; D010940:Plant Proteins; D014157:Transcription Factors