title: Comparative analysis of equine lymphocyte subsets in whole blood and gradient-purified samples.
abstract: In the present study, two methods of lymphocyte preparation, whole blood lysis and Ficoll-Paque separation, prior to FACS analysis were compared. The comparison was done with single and dual-colour staining techniques. Monoclonal antibodies (mAb) against eCD4, eCD5, eCD8 and eMHC class II were used. There was no significant difference in the results obtained by these two methods.
authors.affiliation: Department of Veterinary Internal Medicine, University of Zurich, Switzerland. makens@vetklinik.unizh.ch
authors.forename: M K
authors.initials: MK
authors.lastname: Akens
authors.forename: E
authors.initials: E
authors.lastname: Holznagel
authors.forename: M
authors.initials: M
authors.lastname: Franchini
authors.forename: V
authors.initials: V
authors.lastname: Bracher
mesh_terms: D000818:Animals; D005260:Female; D005434:Flow Cytometry; D006736:Horses / Q000276:immunology*; D016130:Immunophenotyping; D016131:Lymphocyte Subsets / Q000276:immunology*; D008297:Male