title: Interaction of Angeli's salt with cytochrome P450 1A2 distal mutants: an optical absorption spectral study.
abstract: Angeli's salt, Na2N2O3 or O-N=N+-(OH)(O-) in aqueous solution, is known to release NO- or NO., which relaxes vascular tissue and lowers blood pressure. In the liver, the most abundant heme enzyme is cytochrome P450. In the present study, we studied the effect of rat liver cytochrome P450 1A2 (P450 1A2) in regard to its catalysis of the N=N bond scission of Angeli's salt with optical absorption spectra. Also, we examined the contribution of putative distal amino acids of P450 1A2 to the reaction with the salt. We found that wild-type Fe3+ P450 1A2 markedly enhances the N=N scission of the salt up to 100 fold in terms of absorption spectroscopy. A Fe3+ P450 1A2-NO complex with an absorption peak at 435 nm was formed when the salt was added and the complex was then changed to a 6-coordinated Fe2+-NO complex having a 440-nm peak. Glu318Asp, Glu318Ala and Thr319Ala mutants at the putative distal site of P450 1A2 formed a 5-coordinated Fe2+-NO complex having a 400-nm absorption, that was not formed with the wild type. The Glu318Ala mutant, in particular, did not form the Fe3+-NO complex with the addition of Angeli's salt. The presence of L-Cys, reduced glutathione, catalase or superoxide dismutase markedly stabilized the Fe3+ wild type-NO complex. Thus, our data suggests that the N=N bond of Angeli's salt is cleaved with the P450 1A2 active site and NO- or NO. is released. We discuss mechanisms of redox and ligand changes of the P450 heme.
authors.affiliation: Institute for Chemical Reaction Science, Tohoku University, Sendai, Japan.
authors.forename: Y
authors.initials: Y
authors.lastname: Shibata
authors.forename: H
authors.initials: H
authors.lastname: Sato
authors.forename: I
authors.initials: I
authors.lastname: Sagami
authors.forename: T
authors.initials: T
authors.lastname: Shimizu
mesh_terms: D000818:Animals; D019388:Cytochrome P-450 CYP1A2 / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D009154:Mutation; D009573:Nitrites / Q000737:chemistry / Q000378:metabolism*; D011485:Protein Binding; D051381:Rats; D012441:Saccharomyces cerevisiae
chemical_list: D009573:Nitrites; C021229:oxyhyponitrite; D019388:Cytochrome P-450 CYP1A2