Immunocytochemical demonstration of beta 1-subunit of Na+/K(+)-ATPase in the mechanoreceptive Ruffini-like endings of the rat incisor ligament.
The localization of one of the isoforms of Na+/K(+)-ATPase, the beta 1-subunit, was investigated in the periodontal Ruffini endings of rat incisors by light- and electron-microscopic immunocytochemistry. Immunoreactivity for the rat beta 1-subunit followed the pattern of dendritic terminal arborization in the alveolar half of the lingual periodontal ligament. Ultrastructurally, the reaction products were localized in dilatations of axons, possibly the terminals of Ruffini-like endings in the periodontal ligament. No immunoreactivity was seen in Schwann cells. The immunostaining results support the view that the beta 1-subunit of Na+/K(+)-ATPase is the predominant isoform in sensory neurones, and that this protein is a useful marker for periodontal Ruffini-like endings.
Ochi
K
K
Department of Oral Anatomy, Niigata University School of Dentistry, Japan.
Hanada
K
K
Youn
S H
SH
Wakisaka
S
S
Maeda
T
T
D000539:Alveolar Process / Q000294:innervation / Q000648:ultrastructure; D000818:Animals; D001369:Axons / Q000201:enzymology / Q000648:ultrastructure; D015415:Biomarkers / Q000032:analysis; D004396:Coloring Agents; D003712:Dendrites / Q000201:enzymology / Q000648:ultrastructure; D007150:Immunohistochemistry; D007180:Incisor; D007527:Isoenzymes / Q000032:analysis*; D008297:Male; D008465:Mechanoreceptors / Q000201:enzymology / Q000648:ultrastructure*; D016253:Microscopy, Immunoelectron; D009411:Nerve Endings / Q000201:enzymology / Q000648:ultrastructure*; D009475:Neurons, Afferent / Q000201:enzymology / Q000648:ultrastructure; D010513:Periodontal Ligament / Q000294:innervation* / Q000648:ultrastructure; D051381:Rats; D017208:Rats, Wistar; D012583:Schwann Cells / Q000201:enzymology / Q000648:ultrastructure; D000254:Sodium-Potassium-Exchanging ATPase / Q000032:analysis*
D015415:Biomarkers; D004396:Coloring Agents; D007527:Isoenzymes; D000254:Sodium-Potassium-Exchanging ATPase