Increased vascular responsiveness to alpha 2-adrenergic stimulation during NOS inhibition-induced hypertension.
Increased vascular resistance during systemic nitric oxide synthase (NOS) inhibition is dependent on adrenergic vasoconstriction. This study tested the hypothesis that increased vascular sensitivity to adrenergic agonists contributes to this vasoconstriction. Superior mesenteric arteries and thoracic aortae from male Sprague-Dawley rats drinking water containing N omega-nitro-L-arginine (L-NNA; 14 days, 60 mg.kg-1.day-1) and control rats were-cut into helical strips, and endothelium was removed for contractile experiments. L-NNA arteries were more sensitive to UK-14304 (alpha 2-adrenergic agonist) and norepinephrine (NE), whereas responses to phenylephrine (PE) were not different concentration causing 50% maximal response (EC50), L-NNA vs. control: UK-14304, 0.071 vs. 0.71 mumol/l; NE, 1.15 vs. 9.95 nmol/l]. Yohimbine, an alpha 2-selective antagonist, caused a concentration-dependent inhibition of contraction to NE only in L-NNA arteries (EC50 = 6.3 vs. 1.6 nmol/l at 1 nmol/l yohimbine), whereas prazosin shifted NE curves similarly in arteries from both groups. Yohimbine (10 nmol/l) inhibited contractions to UK-14304 (EC50 = 59 mumol/l vs. 17 mumol/l) but not contractions to PE, whereas prazosin inhibited both. These data indicate that L-NNA-induced hypertension leads to increased sensitivity of prazosin-sensitive alpha 2-adrenoceptors, an upregulation that could cause the increased vasoconstrictor response to NE in this model of hypertension.
Kanagy
N L
NL
Department of Physiology, University of New Mexico School of Medicine, Albuquerque 87131, USA.
D000316:Adrenergic alpha-Agonists / Q000494:pharmacology; D000818:Animals; D001013:Aorta, Thoracic / Q000187:drug effects* / Q000502:physiology / Q000503:physiopathology; D001794:Blood Pressure / Q000187:drug effects; D001835:Body Weight / Q000187:drug effects; D000068438:Brimonidine Tartrate; D004791:Enzyme Inhibitors / Q000494:pharmacology; D006973:Hypertension / Q000139:chemically induced / Q000503:physiopathology*; D066298:In Vitro Techniques; D008297:Male; D017538:Mesenteric Artery, Superior / Q000187:drug effects* / Q000502:physiology / Q000503:physiopathology; D009119:Muscle Contraction / Q000187:drug effects; D009131:Muscle, Smooth, Vascular / Q000187:drug effects* / Q000502:physiology / Q000503:physiopathology; D019001:Nitric Oxide Synthase / Q000037:antagonists & inhibitors*; D019335:Nitroarginine / Q000494:pharmacology*; D009638:Norepinephrine / Q000494:pharmacology; D010656:Phenylephrine / Q000494:pharmacology; D011810:Quinoxalines / Q000494:pharmacology; D051381:Rats; D017207:Rats, Sprague-Dawley; D018341:Receptors, Adrenergic, alpha-2 / Q000187:drug effects / Q000502:physiology*; D013599:Systole / Q000187:drug effects; D014661:Vasoconstriction / Q000187:drug effects / Q000502:physiology; D015016:Yohimbine / Q000494:pharmacology
D000316:Adrenergic alpha-Agonists; D004791:Enzyme Inhibitors; D011810:Quinoxalines; D018341:Receptors, Adrenergic, alpha-2; D010656:Phenylephrine; D019335:Nitroarginine; D015016:Yohimbine; D000068438:Brimonidine Tartrate; D019001:Nitric Oxide Synthase; D009638:Norepinephrine