Characterization of a panel of monoclonal antibodies using GalNAc glycosylated peptides and recombinant MUC1.
A panel of 56 murine monoclonal antibodies submitted to the ISOBM TD-4 (MUC1) Workshop was analysed for reactivity against nonglycosylated and in vitro GalNAc glycosylated peptides. Twenty-six antibodies reacted with nonglycosylated MUC1 peptides containing 3-5 tandem repeats. The reactivities of most of the antibodies were not affected by GalNAc glycosylation of the peptides. Antibody #147 (decoded as BCP9) reactivity was inhibited when 3 mol of GalNAc per repeat were incorporated in the peptide (at sites T in GVTSA and ST in GSTAP), whereas the reactivity with GalNAc glycosylated peptides with 2 mol of GalNAc per repeat (sites T in GVTSA and T in GSTAP) was unaffected. This is in agreement with the epitope defined as GSTAP.
Reis
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CA
Department of Oral Diagnostics, School of Dentistry, Faculty of Health Sciences, University of Copenhagen, Denmark.
Hassan
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Bennett
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EP
Clausen
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D000116:Acetylgalactosamine / Q000276:immunology*; D000595:Amino Acid Sequence; D000818:Animals; D000911:Antibodies, Monoclonal / Q000032:analysis*; D004797:Enzyme-Linked Immunosorbent Assay; D006020:Glycopeptides / Q000276:immunology*; D006031:Glycosylation; D006801:Humans; D051379:Mice; D008969:Molecular Sequence Data; D018396:Mucin-1 / Q000276:immunology*; D011994:Recombinant Proteins / Q000276:immunology; D012091:Repetitive Sequences, Nucleic Acid
D000911:Antibodies, Monoclonal; D006020:Glycopeptides; D018396:Mucin-1; D011994:Recombinant Proteins; D000116:Acetylgalactosamine