title: Glycohemoglobin filter assay for doctors' offices based on boronic acid affinity principle.
abstract: We present a new filter assay for the determination of glycohemoglobin as a unique application of the boronic acid affinity principle. With the use of a water-soluble blue-colored boronic acid derivative and a specific precipitation method for hemoglobin, total hemoglobin including bound boronic acid is precipitated and collected on a filter strip before quantification. Hemoglobin and boronic acid are quantified by a dual-wave-length reflectometric measurement, and the result is reported directly as percent glycohemoglobin. The test is simple, quick, and designed as a doctors' office test for the monitoring and management of diabetes. The imprecision of the assay is < 4% over the range 3-18% Hb A1c, and the method is linear up to at least 20% Hb A1c. Comparisons with four well-established glycohemoglobin methods yielded correlation coefficients ranging from 0.94 to 0.99, with slopes from 0.94 to 1.01.
authors.affiliation: Axis Nord, Mørkved, Norway. frank.frantzin@axisbio.no
authors.forename: F
authors.initials: F
authors.lastname: Frantzen
authors.forename: K
authors.initials: K
authors.lastname: Grimsrud
authors.forename: D E
authors.initials: DE
authors.lastname: Heggli
authors.forename: A L
authors.initials: AL
authors.lastname: Faaren
authors.forename: T
authors.initials: T
authors.lastname: Løvli
authors.forename: E
authors.initials: E
authors.lastname: Sundrehagen
mesh_terms: D000818:Animals; D001897:Boronic Acids; D002417:Cattle; D004396:Coloring Agents; D005374:Filtration / Q000295:instrumentation; D006442:Glycated Hemoglobin / Q000032:analysis*; D006801:Humans; D008875:Middle Aged; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013053:Spectrophotometry
chemical_list: D001897:Boronic Acids; D004396:Coloring Agents; D006442:Glycated Hemoglobin