title: High expression of the HMG box factor sox-13 in arterial walls during embryonic development.
abstract: Members of the Sox gene family of transcription factors are defined by the presence of an 80 amino acid homology domain, the High Mobility Group (HMG) box. Here we report the cloning and initial analysis of murine Sox-13 . The 984 amino acids Sox-13 protein contains a single HMG box, a leucine zipper motif and a glutamine-rich stretch. These characteristics are shared with another member of the Sox gene family, Sox-6. High level embryonic expression of Sox-13 occurs uniquely in the arterial walls of 13.5 days post coitum (dpc) mice and later. Low level expression was observed in the inner ear of 13.5 dpc mice and in a limited number of cells in the thymus of 16.5 dpc mice, from which Sox-13 was originally cloned. At 18.5 dpc, Sox-13 is expressed in the tracheal epithelium below the vocal cord and in the hair follicles. The Sox-13 protein binds to the consensus HMG box motif, AACAAAG, but does not transactivate transcription through a concatamer of this motif. Sox-13, like other members of the Sox family likely plays an important role in development.
authors.affiliation: Department of Immunology, University Hospital, PO box 85500, 3508 GA, Utrecht, The Netherlands. j.roose@lab.azu.nl
authors.forename: J
authors.initials: J
authors.lastname: Roose
authors.forename: W
authors.initials: W
authors.lastname: Korver
authors.forename: E
authors.initials: E
authors.lastname: Oving
authors.forename: A
authors.initials: A
authors.lastname: Wilson
authors.forename: G
authors.initials: G
authors.lastname: Wagenaar
authors.forename: M
authors.initials: M
authors.lastname: Markman
authors.forename: W
authors.initials: W
authors.lastname: Lamers
authors.forename: H
authors.initials: H
authors.lastname: Clevers
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001158:Arteries / Q000737:chemistry / Q000196:embryology*; D001324:Autoantigens; D015152:Blotting, Northern; D003001:Cloning, Molecular; D004247:DNA / Q000378:metabolism; D015870:Gene Expression; D006609:High Mobility Group Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D017403:In Situ Hybridization; D051379:Mice; D008969:Molecular Sequence Data; D012333:RNA, Messenger / Q000032:analysis; D013601:T-Lymphocytes / Q000737:chemistry; D013950:Thymus Gland / Q000737:chemistry / Q000196:embryology; D015533:Transcriptional Activation
chemical_list: D001324:Autoantigens; D006609:High Mobility Group Proteins; D012333:RNA, Messenger; C110455:Sox13 protein, mouse; D004247:DNA