title: Introns enable the polyomavirus middle and small T antigens to stimulate the growth of primary rat embryo fibroblasts.
abstract: We constructed spliceable vectors that separately encode polyomavirus MT and ST. The addition of an intron enables MT to transform and to immortalize more efficiently and ST to transiently stimulate the growth of primary rat embryo fibroblasts.
authors.affiliation: Department of Anatomy and Cell Biology, University of Sherbrooke, Quebec, Canada.
authors.forename: C
authors.initials: C
authors.lastname: Asselin
authors.forename: M
authors.initials: M
authors.lastname: Sullivan
authors.forename: M
authors.initials: M
authors.lastname: Bastin
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology; D000952:Antigens, Polyomavirus Transforming / Q000235:genetics* / Q000502:physiology*; D002455:Cell Division / Q000187:drug effects; D002470:Cell Survival / Q000235:genetics; D002472:Cell Transformation, Viral; D005347:Fibroblasts; D005786:Gene Expression Regulation; D005822:Genetic Vectors; D005839:Gentamicins / Q000494:pharmacology; D007438:Introns / Q000235:genetics*; D016297:Mutagenesis, Site-Directed; D016133:Polymerase Chain Reaction; D012326:RNA Splicing; D051381:Rats; D016196:S Phase / Q000235:genetics; D014158:Transcription, Genetic; D014162:Transfection
chemical_list: D000900:Anti-Bacterial Agents; D000952:Antigens, Polyomavirus Transforming; D005839:Gentamicins; C010680:antibiotic G 418