F0F1-ATPase genes from an archaebacterium, Methanosarcina barkeri.
It has been known that an archaebacterium Methanosarcina barkeri strain MS (DSM 800) has a V-type ATPase (Inatomi, K., et al. (1989) J. Biol. Chem. 264, 10954-10959). Here, we report cloning of a cluster of F0F1-ATPase genes from the same organism, the first ever found in archaebacteria. The cluster and encoded subunits exhibit several unusual features such that a gene for delta subunit is lacking, F0-b subunit is unusually large, and gamma subunit is split into two peptide fragments. Attempts to detect F0F1-ATPase proteins and mRNA have been unsuccessful and therefore it is not certain if this gene cluster is really expressed in the cell.
Sumi
M
M
Faculty of Pharmaceutical Sciences of Hokkaido University, Japan.
Yohda
M
M
Koga
Y
Y
Yoshida
M
M
D000595:Amino Acid Sequence; D001482:Base Composition; D019766:Genes, Archaeal; D017021:Methanosarcina barkeri / Q000201:enzymology / Q000235:genetics*; D008969:Molecular Sequence Data; D005810:Multigene Family; D016133:Polymerase Chain Reaction; D011487:Protein Conformation; D006180:Proton-Translocating ATPases / Q000235:genetics*; D015183:Restriction Mapping; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid
D006180:Proton-Translocating ATPases