In vitro proliferation and adhesion of basic fibroblast growth factor-producing fibroblasts on platinum coils.
OBJECTIVE
To evaluate the growth and adhesion characteristics in vitro of genetically modified, basic fibroblast growth factor-producing fibroblasts on platinum detachable coils.


METHODS
Coils of two sizes were coated with laminin, poly-L-lysine, fibronectin, and type I and type IV collagen and were cultured with basic fibroblast growth factor-secreting fibroblasts. Type I collagen strands were inserted in the lumen of some coils. Cellular proliferation and adherence during passage of coils through microcatheters were studied with both light and scanning electron microscopy. Growth factor concentration in the culture medium was measured.


RESULTS
Rapid cellular proliferation was noted on all coated coils except those coated with type IV collagen. Proliferation on uncoated coils was slightly slower than on most coated coils, although confluent cell layers were present on uncoated larger-diameter coils within 48 hours. Cells had a marked propensity to grow between the primary coil windings into the coil lumen, except in coils that contained collagen filaments. Passage through microcatheters caused widespread stripping of cells from the outer surface of coils, especially the uncoated samples. Viable cells remained in the coil lumen. Supernatant contained high concentrations of growth factor.


CONCLUSIONS
Platinum embolic coils are a promising mechanism of cell delivery for stimulation of scar formation or other desirable biologic effects.
Kallmes
D F
DF
Department of Radiology, University of Virginia Health Sciences Center, Charlottesville 22908, USA.
Borland
M K
MK
Cloft
H J
HJ
Altes
T A
TA
Dion
J E
JE
Jensen
M E
ME
Hankins
G R
GR
Helm
G A
GA
D016475:3T3 Cells / Q000166:cytology / Q000502:physiology*; D000818:Animals; D002404:Catheterization / Q000295:instrumentation; D002448:Cell Adhesion; D002455:Cell Division; D003094:Collagen; D004621:Embolization, Therapeutic / Q000295:instrumentation*; D016222:Fibroblast Growth Factor 2 / Q000096:biosynthesis*; D005353:Fibronectins; D007797:Laminin; D051379:Mice; D008855:Microscopy, Electron, Scanning; D010984:Platinum; D011107:Polylysine; D013499:Surface Properties
D005353:Fibronectins; D007797:Laminin; D016222:Fibroblast Growth Factor 2; D011107:Polylysine; D010984:Platinum; D003094:Collagen