Differentiation of human gastric adenocarcinoma cell line MGc80-3 induced by verbascoside.
Verbascoside is a natural antioxidant extracted from Pedicularis striata Pall (Jueyehesen). After being treated with 20 mumol/l verbascoside, the growth curve and mitotic index of human gastric adenocarcinoma MGc80-3 cells decreased remarkably, cell doubling time was delayed, the cellular growth inhibitory rate amounted to 53.2%, cell surface charge assayed by cell electrophoresis obviously changed, the electrophoresis rate dropped from 3.51 microns/s/v/cm to 2.74, i.e., the percent of retardation reached 28.4%. There was a 75% decrease of the tumorigenicity for the treated cells compared with the untreated cells inoculated subcutaneously in BALB/C nude mice. Scanning electron microscopy revealed that the microvilli on the surface of treated cells had been reduced obviously. It confirmed that verbascoside, similar to DMSO, could reverse MGc80-3 cells' malignant phenotypic characteristics and induced redifferentiation of MGc80-3 cells.
Li
J
J
Department of Biology, Lanzhou University, People's Republic of China.
Zheng
Y
Y
Zhou
H
H
Su
B
B
Zheng
R
R
D000230:Adenocarcinoma / Q000473:pathology* / Q000648:ultrastructure; D000818:Animals; D000972:Antineoplastic Agents, Phytogenic / Q000494:pharmacology*; D002454:Cell Differentiation / Q000187:drug effects*; D005960:Glucosides / Q000494:pharmacology*; D006801:Humans; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D008855:Microscopy, Electron, Scanning; D009368:Neoplasm Transplantation; D010636:Phenols; D013274:Stomach Neoplasms / Q000473:pathology* / Q000648:ultrastructure; D014407:Tumor Cells, Cultured; D059808:Polyphenols
D000972:Antineoplastic Agents, Phytogenic; D005960:Glucosides; D010636:Phenols; C058956:acteoside; D059808:Polyphenols