Inhibition of Fe(II) catalyzed linoleic acid oxidation and DNA damage by phosvitin.
The oxidation of linoleic acid catalyzed by Fe(II) is strongly inhibited by phosvitin, while chelation with EDTA, NTA or deferoxamine produced only partial inhibition. Interestingly, the DNA degradation catalyzed by Fe(II) in the presence of H2O2 is also inhibited by phosvitin or deferoxamine. In contrast, chelation of the metal ion with EDTA or NTA enhanced the DNA degradation. The results suggest that the nature of interaction between the metal ion and the complexing agent may be an important factor in the generation of active oxygen intermediates.
Maheswari
S U
SU
Vittal Mallya Scientific Research Foundation, Bangalore, India.
Ramadoss
C S
CS
Krishnaswamy
P R
PR
D000818:Animals; D002384:Catalysis / Q000187:drug effects; D002645:Chickens; D004247:DNA / Q000187:drug effects / Q000378:metabolism; D004249:DNA Damage / Q000187:drug effects*; D005296:Ferrous Compounds / Q000494:pharmacology*; D006863:Hydrogen-Ion Concentration; D019787:Linoleic Acid / Q000037:antagonists & inhibitors* / Q000378:metabolism; D010084:Oxidation-Reduction / Q000187:drug effects; D010774:Phosvitin / Q000494:pharmacology*
D005296:Ferrous Compounds; D004247:DNA; D010774:Phosvitin; D019787:Linoleic Acid