title: Identification of the nonlinearity governing even-order distortion products in cochlear potentials.
abstract: In order to characterize the cochlear transducer nonlinearities which are involved in the generation of the summating potential (SP), we investigated the effect of a change in the electrical operating point of the cochlear transducer on the SP. The electrical operating point of the cochlear transducer was affected by suppressing reversibly the endocochlear potential (EP). This was realized by intravenous injection of furosemide in guinea pig. A differential recording technique was used in the basal turn of the cochlea to measure locally generated even-order distortion products: the SP and the second harmonic component (2F0) of the cochlear microphonics (CM). These potentials were evoked by 2 and 8 kHz stimuli presented at 60 dB SPL. Following furosemide injection, the SP changed polarity twice over time. The zero crossings of the SP coincided with a minimum in the amplitude of 2F0. Concomitantly, the phase of 2F0 shifted about 120 degrees. The changes in the electrical even-order products were comparable to the changes that occurred in a mechanical even-order intermodulation distortion product (the difference tone F2-F1 otoacoustic emission) after furosemide application (Mills et al., J. Acoust. Soc. Am. 94 (1993) 2108-2122). The combined results suggest that only one sigmoidal transfer function may account for the SP, 2F0, and the emission of the difference tone F2-F1, and that shifts in the operating point of the transfer function would be the major cause behind the furosemide-induced changes in the even-order distortion products. The sigmoidal transfer function is likely associated with the mechano-electrical transducer channel at the apical pole of the outer hair cell.
authors.affiliation: Hearing Research Laboratories, Department of Otorhinolaryngology, Utrecht University, The Netherlands. m.g.vanemst@lab.azu.nl
authors.forename: M G
authors.initials: MG
authors.lastname: van Emst
authors.forename: S F
authors.initials: SF
authors.lastname: Klis
authors.forename: G F
authors.initials: GF
authors.lastname: Smoorenburg
mesh_terms: D000161:Acoustic Stimulation; D000200:Action Potentials / Q000187:drug effects / Q000502:physiology*; D000818:Animals; D003051:Cochlea / Q000187:drug effects / Q000502:physiology*; D003055:Cochlear Microphonic Potentials / Q000187:drug effects / Q000502:physiology*; D004232:Diuretics / Q000494:pharmacology; D005260:Female; D005665:Furosemide / Q000008:administration & dosage / Q000494:pharmacology; D006168:Guinea Pigs; D018072:Hair Cells, Auditory, Outer / Q000187:drug effects / Q000502:physiology; D007275:Injections, Intravenous; D017084:Otoacoustic Emissions, Spontaneous
chemical_list: D004232:Diuretics; D005665:Furosemide