title: Neurohormonal stimulation of histamine release from neuroendocrine cells of the human adenomatous prostate.
abstract: BACKGROUND
  Neuroendocrine cells (NE) constitute a population of highly specialized cells in prostatic glands; histamine has never been described in these cells. This article shows the presence and the regulation of release of histamine in NE.
  
  
  METHODS
  In 21 prostatic adenomas, NE were identified by specific antisera against neuroendocrine markers (chromogranin-A, synaptophysin), histamine, and histidine decarboxylase (HDC); a rate HDC-cDNA probe was used to detect this enzyme by in situ hybridization.
  
  
  RESULTS
  Immunoreactive cells for chromogranin-A, histamine, and HDC were found among luminal epithelial glandular cells. Similar cells were also labeled with the HDC-cDNA probe. Glandular cells, isolated from prostatic adenomas, were shown to contain histamine (7-40 pmol/mg cellular protein). L(-) norepinephrine causes a time-dependent (t1/2 = 22 min) histamine release; the alpha 1-receptor antagonists WB-4101 and YM-617 specifically inhibited this release, in agreement with a mediation by alpha 1-adrenoreceptor subtype.
  
  
  CONCLUSIONS
  There is some evidence for the presence in prostatic adenomas of histamine-forming cells of neuroendocrine type; histamine release from these cells is under the control of alpha 1-adrenoreceptor subtype.
authors.affiliation: Unité Fonctionnelle d'Exploration de la Fibre Lisse, CHU Carémeau, Nimes, France.
authors.forename: A
authors.initials: A
authors.lastname: Polge
authors.forename: C
authors.initials: C
authors.lastname: Gaspard
authors.forename: N
authors.initials: N
authors.lastname: Mottet
authors.forename: C
authors.initials: C
authors.lastname: Guitton
authors.forename: J C
authors.initials: JC
authors.lastname: Boyer
authors.forename: A
authors.initials: A
authors.lastname: Choquet
authors.forename: S
authors.initials: S
authors.lastname: Combettes
authors.forename: E
authors.initials: E
authors.lastname: Bancel
authors.forename: P
authors.initials: P
authors.lastname: Costa
authors.forename: J P
authors.initials: JP
authors.lastname: Bali
mesh_terms: D000316:Adrenergic alpha-Agonists / Q000494:pharmacology; D000317:Adrenergic alpha-Antagonists / Q000494:pharmacology; D000319:Adrenergic beta-Antagonists / Q000494:pharmacology; D000818:Animals; D002217:Carbachol / Q000494:pharmacology; D015342:DNA Probes; D006636:Histamine Release / Q000502:physiology*; D006640:Histidine Decarboxylase / Q000235:genetics; D006801:Humans; D007124:Immunoenzyme Techniques; D017403:In Situ Hybridization; D008297:Male; D018721:Muscarinic Agonists / Q000494:pharmacology; D009638:Norepinephrine / Q000494:pharmacology; D011224:Prazosin / Q000378:metabolism; D011467:Prostate / Q000187:drug effects / Q000378:metabolism*; D011470:Prostatic Hyperplasia / Q000378:metabolism* / Q000473:pathology; D051381:Rats; D018340:Receptors, Adrenergic, alpha-1 / Q000378:metabolism; D014316:Tritium; D014407:Tumor Cells, Cultured; D003189:p-Methoxy-N-methylphenethylamine / Q000494:pharmacology
chemical_list: D000316:Adrenergic alpha-Agonists; D000317:Adrenergic alpha-Antagonists; D000319:Adrenergic beta-Antagonists; D015342:DNA Probes; D018721:Muscarinic Agonists; D018340:Receptors, Adrenergic, alpha-1; D014316:Tritium; D003189:p-Methoxy-N-methylphenethylamine; D002217:Carbachol; D006640:Histidine Decarboxylase; D009638:Norepinephrine; D011224:Prazosin