The influence of the C1-inhibitor BERINERT and the protein-free haemodialysate ACTIHAEMYL20% on the evolution of the depth of scald burns in a porcine model.
Standardized deep partial-thickness burns were inflicted on domestic pigs by scalding 30 per cent of the skin surface for 25 s with 75 degrees C hot water. The animals (n = 18; weight 25-35 kg) were divided into three groups: I, control group (n = 6), Ringer's lactate only; II, haemodialysate group (n = 6), Ringer's lactate and a protein-free haemodialysate of calf-blood (ACTIHAEMYL20%; AH) and III, C1-inhibitor group (n = 6), Ringer's lactate and C1-inhibitor (C1-INH; BERINERT). Skin biopsies were taken at defined time points (4, 28, 52 and 76 h) and investigated histologically. Depth of burn was determined morphometrically after coloration with a modified MTT-staining on frozen sections of the skin biopsies. Fluid therapy with C1-INH decelerated significantly the progression of the burn wound in the postburn-period compared to Ringer's lactate alone. In comparison with C1-INH, the treatment with AH demonstrated a less beneficial influence on the depth of scald burns. The favourable effects of C1-INH are explained by the protection of the dermal microcirculation during the acute phase of thermal injury.
Henze
U
U
Institute of Pathology, Aachen University of Technology, Germany.
Lennartz
A
A
Hafemann
B
B
Goldmann
C
C
Kirkpatrick
C J
CJ
Klosterhalfen
B
B
D000183:Actihaemyl / Q000008:administration & dosage*; D000818:Animals; D001707:Biopsy, Needle; D002056:Burns / Q000188:drug therapy / Q000473:pathology*; D003174:Complement C1 Inactivator Proteins / Q000008:administration & dosage*; D050718:Complement C1 Inhibitor Protein; D004195:Disease Models, Animal; D018450:Disease Progression; D011897:Random Allocation; D012016:Reference Values; D012867:Skin / Q000187:drug effects* / Q000473:pathology; D018709:Statistics, Nonparametric; D013552:Swine; D014945:Wound Healing / Q000187:drug effects* / Q000502:physiology
D003174:Complement C1 Inactivator Proteins; D050718:Complement C1 Inhibitor Protein; D000183:Actihaemyl