title: A mathematical model of the influence of salivary urea on the pH of fasted dental plaque and on the changes occurring during a cariogenic challenge.
abstract: Urea diffusing from saliva into dental plaque is converted to ammonia and carbon dioxide by bacterial ureases. The influence of normal salivary urea levels on the pH of fasted plaque and on the depth and duration of a Stephan curve is uncertain. A numerical model which simulates a cariogenic challenge (a 10% sucrose rinse alone or one followed by use of chewing-gum with or without sugar) was modified to include salivary urea levels from 0 to 30 mmol/l. It incorporated: site-dependent exchange between bulk saliva and plaque surfaces via a salivary film; sugar and urea diffusion into plaque; pH-dependent rates of acid formation and urea breakdown; diffusion and dissociation of end-products and other buffers (acetate, lactate, phosphate, ammonia and carbonate); diffusion of protons and other ions; equilibration with fixed and mobile buffers; and charge-coupling between ionic flows. The Km (2.12 mmol/l) and Vmax (0.11 micromol urea/min/mg dry weight) values for urease activity and the pH dependence of Vmax were taken from the literature. From the results, it is predicted that urea concentrations normally present in saliva (3-5 mmol/l) will increase the pH at the base of a 0.5-mm-thick fasted plaque by up to 1 pH unit, and raise the pH minimum after a sucrose rinse or sugar-containing chewing-gum by at least half a pH unit. The results suggest that plaque cariogenicity may be inversely related to salivary urea concentrations, not only when the latter are elevated because of disease, but even when they are in the normal range.
authors.affiliation: MRC Dental Group, The Dental School, Bristol, UK.
authors.forename: G H
authors.initials: GH
authors.lastname: Dibdin
authors.forename: C
authors.initials: C
authors.lastname: Dawes
mesh_terms: D000085:Acetates / Q000494:pharmacology; D000143:Acids / Q000378:metabolism; D000641:Ammonia / Q000378:metabolism / Q000494:pharmacology; D001419:Bacteria / Q000201:enzymology; D002021:Buffers; D002245:Carbon Dioxide / Q000378:metabolism; D002254:Carbonates / Q000494:pharmacology; D002326:Cariogenic Agents / Q000008:administration & dosage / Q000494:pharmacology*; D002638:Chewing Gum; D003731:Dental Caries / Q000378:metabolism / Q000503:physiopathology*; D003741:Dental Deposits / Q000378:metabolism / Q000503:physiopathology; D003773:Dental Plaque / Q000378:metabolism / Q000503:physiopathology*; D019422:Dietary Sucrose / Q000008:administration & dosage / Q000494:pharmacology*; D004058:Diffusion; D004563:Electrochemistry; D005215:Fasting; D005544:Forecasting; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007477:Ions; D007773:Lactates / Q000494:pharmacology; D008954:Models, Biological; D010710:Phosphates / Q000494:pharmacology; D011522:Protons; D012463:Saliva / Q000378:metabolism* / Q000502:physiology; D014508:Urea / Q000378:metabolism*; D014510:Urease / Q000378:metabolism
chemical_list: D000085:Acetates; D000143:Acids; D002021:Buffers; D002254:Carbonates; D002326:Cariogenic Agents; D002638:Chewing Gum; D019422:Dietary Sucrose; D007477:Ions; D007773:Lactates; D010710:Phosphates; D011522:Protons; D002245:Carbon Dioxide; D000641:Ammonia; D014508:Urea; D014510:Urease