Structure of an acidic exopolysaccharide of Burkholderia pseudomallei.
A recently described water-soluble exopolysaccharide of Burkholderia pseudomallei recognized by the IgG 1 monoclonal antibody 3015 [Steinmetz, I., Rohde, M. & Brenneke, B. (1995) Infect. Immun. 63, 3959-3965] was isolated by repetitive ethanol-precipitation steps and by anion-exchange chromatography. The structure of the polysaccharide was determined by a combination of chemical-derivatization and mass-spectrometric techniques (compositional and methylation analysis, GC/MS, and electrospray-ionization-MS/MS of reduced and permethylated hydrolytic fragments), and two-dimensional 1H-NMR methods (COSY, TOCSY and NOESY) and confirmed by isolation and structural characterization of the depolymerized repeating unit of the polysaccharide. The combined structural data established a linear tetrasaccharide repeating unit consisting of three galactose residues, one bearing a 2-linked O-acetyl group, and a 3-deoxy-D-manno-2-octulosonic acid residue. [-->3)-beta-D-Galp2Ac-(1-->4)-alpha-D-Galp-(1-->3)-beta-D-Galp-(1- ->5)-beta-Kdo-(2-->]n
Nimtz
M
M
GBF, Braunschweig, Germany.
Wray
V
V
Domke
T
T
Brenneke
B
B
Häussler
S
S
Steinmetz
I
I
D016957:Burkholderia pseudomallei / Q000737:chemistry*; D011135:Polysaccharides, Bacterial / Q000737:chemistry* / Q000302:isolation & purification
D011135:Polysaccharides, Bacterial