title: Calmodulin-dependent protein kinase IV during T-cell development.
abstract: In a primary cell culture system of fetal rat brain, the calmodulin-dependent protein-kinase IV (CaMKIV) could be induced by the thyroid hormone T3 in a time- and concentration-dependent manner, provided the tissue was excised not later than day 15 of gestation (E15) (Krebs et al., J. Biol. Chem. 271, 11055, 1996). We report here that in the fetal thymus CaMKIV could not be detected earlier than day 16 of gestation and that the expression of this enzyme was fully upregulated at day 18. In mouse fetal thymus organ culture (FTOC) of day 14 embryonic thymus, CaMKIV could not be detected, even after several days of culture if a minimal culture medium lacking fetal calf serum was used. However, after addition of fetal calf serum to the culture medium the expression of CaMKIV could be specifically induced. Furthermore, it could also be shown that during T-cell development in the adult murine thymus the expression of CaMKIV was tightly regulated. Taken together, these results demonstrate that the expression of CaMKIV, an enzyme involved in the regulation of Ca(2+)-dependent gene expression, is itself under stringent regulatory control during tissue development.
authors.affiliation: Institute of Biochemistry, Swiss Federal Institute of Technology (ETH), Zurich, Switzerland.
authors.forename: J
authors.initials: J
authors.lastname: Krebs
authors.forename: A
authors.initials: A
authors.lastname: Wilson
authors.forename: P
authors.initials: P
authors.lastname: Kisielow
mesh_terms: D000818:Animals; D054734:Calcium-Calmodulin-Dependent Protein Kinase Type 4; D017871:Calcium-Calmodulin-Dependent Protein Kinases / Q000096:biosynthesis*; D002478:Cells, Cultured; D015971:Gene Expression Regulation, Enzymologic; D006728:Hormones; D051379:Mice; D009924:Organ Culture Techniques; D013601:T-Lymphocytes / Q000201:enzymology*; D013687:Telencephalon / Q000166:cytology / Q000201:enzymology; D013950:Thymus Gland / Q000166:cytology / Q000196:embryology / Q000254:growth & development*
chemical_list: D006728:Hormones; D054734:Calcium-Calmodulin-Dependent Protein Kinase Type 4; D017871:Calcium-Calmodulin-Dependent Protein Kinases; C517685:Camk4 protein, mouse; C517686:Camk4 protein, rat