title: Neuronal NOS gene expression in gastrointestinal myenteric neurons and smooth muscle cells.
abstract: Nitric oxide synthase (NOS) has been characterized in different tissues, and its localization has been suggested in different neuronal tissues, including the myenteric neurons and other nonneuronal cells. The present study examined the distribution of the neuronal NOS (nNOS) mRNA in different tissues of the opossum gastrointestinal tract, internal anal sphincter (IAS) smooth muscle cells, and myenteric neurons using slot-blot and Northern blot hybridization techniques with a specific rat brain nNOS cDNA probe. Significant levels of nNOS gene expression were found in both smooth muscle cells and myenteric neurons of the opossum IAS. This finding was confirmed by reverse transcriptase-polymerase chain reaction analysis of the RNA obtained from cultured opossum IAS smooth muscle cells and myenteric neurons and also from human intestinal smooth muscle and neuroblastoma cell lines. Pyloric sphincter had the highest level of nNOS gene expression compared with other gastrointestinal tissues. There was no significant difference in the nNOS gene expression between other sphincteric and nonsphincteric tissues examined. The present study shows the presence of nNOS gene expression in both neurons and smooth muscle cells. The higher levels of nNOS gene expression in the pyloric sphincter compared with other tissues may have pathophysiological significance in some disease conditions.
authors.affiliation: Department of Medicine, Thomas Jefferson University, Philadelphia, Pennsylvania 19107, USA.
authors.forename: S
authors.initials: S
authors.lastname: Chakder
authors.forename: A
authors.initials: A
authors.lastname: Bandyopadhyay
authors.forename: S
authors.initials: S
authors.lastname: Rattan
mesh_terms: D000818:Animals; D015152:Blotting, Northern; D001921:Brain / Q000201:enzymology; D002478:Cells, Cultured; D004064:Digestive System / Q000201:enzymology* / Q000294:innervation*; D006801:Humans; D009130:Muscle, Smooth / Q000201:enzymology* / Q000294:innervation; D009197:Myenteric Plexus / Q000201:enzymology*; D009447:Neuroblastoma / Q000201:enzymology; D009474:Neurons / Q000201:enzymology*; D019001:Nitric Oxide Synthase / Q000032:analysis / Q000096:biosynthesis*; D009893:Opossums; D009928:Organ Specificity; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D014158:Transcription, Genetic; D014407:Tumor Cells, Cultured
chemical_list: D012333:RNA, Messenger; D019001:Nitric Oxide Synthase