title: Leukocyte emigration in normal calves and calves with leukocyte adhesion deficiency.
abstract: The emigration of leukocytes from calves with beta 2 integrin deficiency (BLAD) into bronchoalveolar spaces and scraped tissues was compared to that of normal calves. Polymorphonuclear neutrophils were found in bronchoalveolar lavage fluid from BLAD-affected calves showing chronic pneumonia. The neutrophils were complement receptor type 3 (CR3)-negative when characterized by flow cytometric analysis using anti-CD18 monoclonal antibody. Chemiluminescent response mediated by CR3 in neutrophils isolated from bronchoalveolar lavage fluid from BLAD-calves showed similar findings obtained from CR3-deficient neutrophils. Neutrophils from normal calves migrated into scraped tissue which was prepared in an upper gluteal surface area, whereas few leukocytes from calves with BLAD migrated to the scraped tissue, evaluated by skin window (Rebuck) method. These findings confirmed the extravasation of CR3-deficient leukocytes into bronchoalveolar lumen in BLAD calves, and demonstrated in vivo characteristics of extravasating property of normal and CR3-deficient neutrophils into scraped tissues.
authors.affiliation: Department of Animal Health, School of Veterinary Medicine, Rakuno Gakuen University, Hokkaido, Japan.
authors.forename: H
authors.initials: H
authors.lastname: Nagahata
authors.forename: A
authors.initials: A
authors.lastname: Masuyama
authors.forename: M
authors.initials: M
authors.lastname: Masue
authors.forename: M
authors.initials: M
authors.lastname: Yuki
authors.forename: H
authors.initials: H
authors.lastname: Higuchi
authors.forename: H
authors.initials: H
authors.lastname: Ohtsuka
authors.forename: T
authors.initials: T
authors.lastname: Kurosawa
authors.forename: H
authors.initials: H
authors.lastname: Sato
authors.forename: H
authors.initials: H
authors.lastname: Noda
mesh_terms: D000818:Animals; D001992:Bronchoalveolar Lavage Fluid / Q000166:cytology; D018821:CD18 Antigens / Q000032:analysis / Q000378:metabolism; D002417:Cattle; D002418:Cattle Diseases / Q000097:blood* / Q000473:pathology; D002448:Cell Adhesion / Q000502:physiology; D002465:Cell Movement / Q000502:physiology*; D005260:Female; D005434:Flow Cytometry / Q000662:veterinary; D018370:Leukocyte-Adhesion Deficiency Syndrome / Q000097:blood / Q000473:pathology / Q000662:veterinary*; D008163:Luminescent Measurements; D009504:Neutrophils / Q000737:chemistry / Q000473:pathology* / Q000502:physiology; D011014:Pneumonia / Q000097:blood / Q000473:pathology / Q000662:veterinary; D011650:Pulmonary Alveoli / Q000737:chemistry / Q000473:pathology; D011951:Receptors, Complement / Q000032:analysis / Q000172:deficiency / Q000378:metabolism; D012867:Skin / Q000166:cytology
chemical_list: D018821:CD18 Antigens; D011951:Receptors, Complement