Modified Evans blue fluorimetry for determination of pulmonary vascular permeability in rats sustaining burns, and delayed fluid resuscitation of burn shock.
The present experiment used modified Evans blue fluorimetry to determine changes in pulmonary vascular permeability using a delayed resuscitation model of burn shock in rats. The results showed that pulmonary vascular permeability in the immediate resuscitation (IR) group regressed to normal 12-24 h following the burn whereas it regressed slowly in the delayed resuscitation (DR) group. The study showed that Evans blue fluorimetry is a reliable and sensitive method for determining pulmonary vascular permeability, and dimethyl formamide is a preferable extracting solution which provides a quick and convenient means for scientific research.
Lu
W
W
Burn Center, Changhai Hospital, People's Republic of China.
Chen
Y
Y
Xia
Z
Z
Fang
Z
Z
D000818:Animals; D002056:Burns / Q000175:diagnosis / Q000503:physiopathology* / Q000628:therapy; D002199:Capillary Permeability; D004396:Coloring Agents / Q000493:pharmacokinetics*; D004195:Disease Models, Animal; D005070:Evans Blue / Q000493:pharmacokinetics*; D005470:Fluorometry / Q000379:methods*; D016014:Linear Models; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley; D012016:Reference Values; D015532:Rehydration Solutions / Q000008:administration & dosage*; D012151:Resuscitation / Q000379:methods; D012680:Sensitivity and Specificity; D012774:Shock, Traumatic / Q000175:diagnosis / Q000503:physiopathology* / Q000628:therapy
D004396:Coloring Agents; D015532:Rehydration Solutions; D005070:Evans Blue