title: Estrogen receptor diminishes DNA-binding activities of chicken GATA-1 and CACCC-binding proteins.
abstract: The estrogen receptor (ER) repressed erythroid differentiation and erythroid-specific gene expression. In this study, we investigated the effect of ER alpha (referred to throughout as ER) on DNA-binding activities of transcription factors involved in regulating the expression of erythroid-specific genes, and, in particular, the histone H5 gene. Using electrophoretic mobility shift assays, we found that in the presence of rabbit reticulocyte lysate, human ER reduced the binding activities of chicken immature erythrocyte nuclear extracted proteins to GATA and CACCC sites in the H5 promoter and enhancer. In contrast, the binding activities of NF1 and Sp1 were not affected by ER. Binding of ER to an estrogen response element was enhanced by addition of rabbit reticulocyte lysate. This lysate was also necessary for ER to diminish the DNA-binding activity of GATA-1. These results suggest that additional factor(s) are necessary for full ER function. Both GATA-1 and CACCC-binding proteins are critical for the developmentally regulated expression of erythroid-specific genes. We hypothesize that interference in DNA-binding activities of GATA-1 and CACCC-binding proteins is the mechanism by which the ER inhibits regulation of these genes.
authors.affiliation: Department of Biochemistry and Molecular Biology, Faculty of Medicine, University of Manitoba, Winnipeg, Canada.
authors.forename: L T
authors.initials: LT
authors.lastname: Holth
authors.forename: J M
authors.initials: JM
authors.lastname: Sun
authors.forename: A S
authors.initials: AS
authors.lastname: Coutts
authors.forename: L C
authors.initials: LC
authors.lastname: Murphy
authors.forename: J R
authors.initials: JR
authors.lastname: Davie
mesh_terms: D000818:Animals; D017123:Bacteriophage T7 / Q000235:genetics; D002645:Chickens; D004268:DNA-Binding Proteins / Q000235:genetics / Q000378:metabolism*; D004586:Electrophoresis / Q000379:methods; D004742:Enhancer Elements, Genetic; D004912:Erythrocytes / Q000378:metabolism; D050981:Erythroid-Specific DNA-Binding Factors; D050982:GATA1 Transcription Factor; D006657:Histones / Q000235:genetics / Q000378:metabolism*; D006801:Humans; D051839:NFI Transcription Factors; D011401:Promoter Regions, Genetic; D014176:Protein Biosynthesis; D011817:Rabbits; D011960:Receptors, Estrogen / Q000235:genetics / Q000378:metabolism*; D016329:Sp1 Transcription Factor / Q000378:metabolism; D014157:Transcription Factors / Q000378:metabolism*; D014158:Transcription, Genetic
chemical_list: D004268:DNA-Binding Proteins; D050981:Erythroid-Specific DNA-Binding Factors; D050982:GATA1 Transcription Factor; C494688:GATA1 protein, human; D006657:Histones; D051839:NFI Transcription Factors; D011960:Receptors, Estrogen; D016329:Sp1 Transcription Factor; D014157:Transcription Factors; C080333:transcription factor nuclear factor 1