The influence of diet on the mucin carbohydrates in the chick intestinal tract.
The presence of nutrients in the intestinal lumen is a major factor influencing bacterial colonization in poultry. This study was conducted to investigate the effects of poultry feed on viscosity of intestinal contents and on mucin carbohydrates by comparing jejunal supernatants and the histochemical composition of goblet cells in chicks reared to 5 weeks of age on either a conventional maize-based diet or a wheat-based diet or a wheat diet supplemented with 0.1% xylanase. Regional differences in the distribution of the neutral, carboxylated and sulphated mucins were demonstrated using conventional histochemical techniques, while a panel of lectins was used to study alterations in glycoconjugate synthesis of mucins in chicks fed different diets. 1) Feeding a diet supplemented with xylanase lowered the viscosity but increased the amount of neutral, carboxylated and sulphated mucins in the jejunum. 2) In chicks fed a maize-based diet, neutral mucins increased in the surface and upper crypt goblet cells of the small and large intestines but decreased in the caecum. 3) Feeding a diet supplemented with xylanase modified crypt-surface glycosylation of N-acetylglucosamine residues and resulted in loss of sialic acid residues in the small and large intestines. These results indicate that the constituents of poultry feed, in particular the consumption of a diet supplemented with xylanase, lead to changes in intestinal viscosity and mucin composition which are associated with alterations in the goblet cell glycoconjugates of the chick intestinal tract.
Sharma
R
R
University of Southampton, UK. rps@soton.ac.uk
Fernandez
F
F
Hinton
M
M
Schumacher
U
U
D000821:Animal Feed; D000818:Animals; D002645:Chickens / Q000502:physiology*; D006001:Glycoconjugates / Q000032:analysis; D006863:Hydrogen-Ion Concentration; D007413:Intestinal Mucosa / Q000737:chemistry*; D037102:Lectins; D009077:Mucins / Q000737:chemistry*; D037121:Plant Lectins; D014908:Triticum; D014783:Viscosity; D003313:Zea mays
D006001:Glycoconjugates; D037102:Lectins; D009077:Mucins; D037121:Plant Lectins