P1GF-saporin fusion protein: a potential anti-angiogenic agent.
Vascularization is an important step in tumor growth and metastasis. Tumor neovascularization can be considered, therefore, as a good target for antineoplastic therapy. In order to target saporin, a powerful plant toxin, in proximity of the tumor we fused the saporin coding sequence to that for placental growth factor-2 (P1GF-2). P1GF is an angiogenic factor involved in tumor neovascularization. The fusion protein P1GF-2-saporin was obtained by transient transfection of mammalian cells and released in the culture medium as a 57.5 kDa polypeptide. Selectivity and cytotoxic activity are reported as a preliminary step towards the evaluation of its in vivo antitumor activity.
Chiaramonte
R
R
Department of Biomedical Sciences and Technologies, University of Milan, Italy.
Polizzi
D
D
Bartolini
E
E
Petroni
D
D
Comi
P
P
D016475:3T3 Cells / Q000187:drug effects; D000818:Animals; D015153:Blotting, Western; D019556:COS Cells / Q000187:drug effects; D004730:Endothelium, Vascular / Q000166:cytology / Q000187:drug effects*; D006031:Glycosylation; D000922:Immunotoxins; D051379:Mice; D009699:N-Glycosyl Hydrolases; D009389:Neovascularization, Pathologic / Q000517:prevention & control; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism / Q000494:pharmacology*; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000378:metabolism / Q000494:pharmacology*; D054789:Ribosome Inactivating Proteins, Type 1; D000076985:Saporins; D014162:Transfection; D016212:Transforming Growth Factor beta / Q000235:genetics / Q000378:metabolism / Q000494:pharmacology*
D000922:Immunotoxins; D010940:Plant Proteins; D011993:Recombinant Fusion Proteins; D054789:Ribosome Inactivating Proteins, Type 1; D016212:Transforming Growth Factor beta; D009699:N-Glycosyl Hydrolases; D000076985:Saporins