title: Hydrolysis of glycyrrhetyl mono-glucuronide to glycyrrhetic acid by glycyrrhetyl mono-glucuronide beta-D-glucuronidase of Eubacterium sp. GLH.
abstract: Glycyrrhetyl mono-glucuronide (GAMG) is an intermediate in the hydrolysis of glycyrrhizin (GL) to glycyrrhetic acid (GA). An enzyme responsible for its hydrolysis, characterized as a GAMG beta-D-glucuronidase of Eubacterium sp. (species) GLH, has been isolated from human intestinal bacteria. The pattern of GAMG beta-D-glucuronidase activity was different from that of GL beta-D-glucuronidase activity by Butyl-Toyopearl 650 S column chromatography. Thus, these enzymes showed differences in the purification ratio and substrate specificity. After this step, GAMG beta-D-glucuronidase was completely separated from GL beta-D-glucuronidase by gel filtration through Toyopearl HW-55 S, indicating that the GAMG beta-D-glucuronidase is a novel type of beta-D-glucuronidase which hydrolyzes one glucuronic acid linkage of GA.
authors.affiliation: Faculty of Pharmaceutical Sciences, Toyama Medical and Pharmaceutical University, Sugitani, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Akao
mesh_terms: D002850:Chromatography, Gel; D002855:Chromatography, Thin Layer; D005051:Eubacterium / Q000201:enzymology*; D005966:Glucuronidase / Q000378:metabolism*; D006034:Glycyrrhetinic Acid / Q000031:analogs & derivatives* / Q000378:metabolism; D006801:Humans; D006868:Hydrolysis; D007422:Intestines / Q000382:microbiology
chemical_list: C052400:glycyrrhetyl 3-monoglucuronide; D005966:Glucuronidase; D006034:Glycyrrhetinic Acid