Quality control of Kato slide counts for Schistosoma mansoni: a review of 12 years' experience in Kenya.
A total of 19 annual or biannual audits were performed over a 12-year period by an independent microscopist on randomized subsamples of Kato slides examined for Schistosoma mansoni eggs by Kenyan microscopists from the Division of Vector-borne Diseases (DVBD). The recounts were invariably lower than the originals owing to some deterioration of the preparations between counts, but the two were strongly correlated: significant regressions of recounts on counts taking up 80-90% of the observed variance. Observer bias differed significantly between microscopists but remained stable over time, whereas repeatability of recounts on counts dropped slightly in periods of maximum work load but did not vary systematically with time. Approximately 7% of the counts and recounts disagreed on the presence or absence of eggs, but less than a third of these were negatives that were found positive on recount. False negatives dropped to 1.3% if duplicate counts were considered. The performance of the Kenyan microscopists was remarkably high and consistent throughout the 12-year period. This form of quality control is suitable for projects where limited funds preclude full-time supervisors using more sophisticated systems.
Sturrock
R F
RF
Department of Medical Parasitology, London School of Hygiene and Tropical Medicine, England.
Ouma
J H
JH
Kariuki
H C
HC
Thiongo
F W
FW
Koech
D K
DK
Butterworth
A E
AE
D000818:Animals; D005243:Feces / Q000469:parasitology; D007630:Kenya; D010270:Parasite Egg Count / Q000379:methods* / Q000592:standards*; D011786:Quality Control; D011897:Random Allocation; D012015:Reference Standards; D012044:Regression Analysis; D012550:Schistosoma mansoni / Q000302:isolation & purification