Low-dose automated electron tomography: a recent implementation.
Low-dose automated tomography has been implemented on a 400-kV JEOL intermediate voltage electron microscope. Instrumentation and procedures for automatic tomographic series data collection are described. Difficulties encountered and ways to overcome them are discussed. A low-dose tomographic projection series of a triad junction of frog sartorius muscle was semiautomatically collected and a 3-D reconstruction of this organelle was made.
Rath
B K
BK
Wadsworth Center, New York State Department of Health, Albany, New York 12201-0509, USA.
Marko
M
M
Radermacher
M
M
Frank
J
J
D000818:Animals; D001331:Automation; D002138:Calibration; D007091:Image Processing, Computer-Assisted; D008854:Microscopy, Electron / Q000295:instrumentation / Q000379:methods*; D018482:Muscle, Skeletal / Q000648:ultrastructure; D011898:Ranidae; D012984:Software; D014054:Tomography / Q000295:instrumentation / Q000379:methods*