A hydroxypyridinone (CP94) enhances protoporphyrin IX formation in 5-aminolaevulinic acid treated cells.
Different cell lines were given photodynamic treatment with 5-aminolaevulinic acid (ALA) and light. In addition, the iron chelator 1,2-diethyl-3-hydroxypyridin-4-one (CP94) was used. The porphyrin species produced was spectrofluorimetrically identified as protoporphyrin IX. All the cell lines responded to treatment, including a multidrug resistance gene expressing bladder cancer line and, to a lesser degree, cells derived from untransformed human skin fibroblasts. CP94 enhanced both porphyrin fluorescence, total porphyrin content and photosensitivity of the cells. CCD fluorescence microscopy showed a granular extranuclear porphyrin fluorescence distribution for all the cell lines involved, but the untransformed cells showed a distribution pattern different from the ones seen in the other cells.
Bech
O
O
Institute for Cancer Research, Montebello, Oslo, Norway.
Phillips
D
D
Moan
J
J
MacRobert
A J
AJ
D000622:Aminolevulinic Acid / Q000494:pharmacology* / Q000633:toxicity; D002460:Cell Line; D003624:Darkness; D006801:Humans; D007502:Iron Chelating Agents / Q000737:chemistry / Q000494:pharmacology* / Q000633:toxicity; D008856:Microscopy, Fluorescence; D015394:Molecular Structure; D011355:Prodrugs / Q000494:pharmacology* / Q000633:toxicity; D011524:Protoporphyrins / Q000096:biosynthesis*; D011728:Pyridones / Q000737:chemistry / Q000494:pharmacology* / Q000633:toxicity; D013050:Spectrometry, Fluorescence
D007502:Iron Chelating Agents; D011355:Prodrugs; D011524:Protoporphyrins; D011728:Pyridones; C068543:1,2-diethyl-3-hydroxypyridin-4-one; D000622:Aminolevulinic Acid; C028025:protoporphyrin IX