Yeast gene YOR137c is involved in the activation of the yeast plasma membrane H+-ATPase by glucose.
Glucose triggers transcriptional and post-transcriptional mechanisms that increase the level and activity of Saccharomyces cerevisiae plasma membrane H+-ATPase. We have studied the post-transcriptional activation of the enzyme by glucose and have found that the YOR137c gene product is implicated in this activation. Deletion of YOR137c does not affect the level of Pma1 at the plasma membrane, but disturbs the glucose-triggered Vmax increase of the enzyme. We propose that at least two independent mechanisms are involved in glucose activation of the H+-ATPase.
de la Fuente
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Departamento de Bioquímica, Facultad de Medicina, Universidad Autónoma de Madrid and Instituto de Investigaciones Biomédicas del Consejo Superior de Investigaciones Científicas, Spain.
Maldonado
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AM
Portillo
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D002462:Cell Membrane / Q000201:enzymology; D003001:Cloning, Molecular; D004789:Enzyme Activation / Q000187:drug effects; D005800:Genes, Fungal / Q000235:genetics / Q000502:physiology*; D005947:Glucose / Q000494:pharmacology*; D007700:Kinetics; D006180:Proton-Translocating ATPases / Q000378:metabolism*; D012441:Saccharomyces cerevisiae / Q000201:enzymology* / Q000235:genetics; D017384:Sequence Deletion
D006180:Proton-Translocating ATPases; D005947:Glucose