Association of borderline oxacillin-susceptible strains of Staphylococcus aureus with surgical wound infections.
Staphylococcus aureus isolates which produce type A staphylococcal beta-lactamase have been associated with wound infections complicating the use of cefazolin prophylaxis in surgery. To further evaluate this finding, 215 wound isolates from 14 cities in the United States were characterized by antimicrobial susceptibility and beta-lactamase type and correlated with the preoperative prophylactic regimen. Borderline-susceptible S. aureus isolates of phage group 5 (BSSA-5), which produce large amounts of type A beta-lactamase and exhibit borderline susceptibility to oxacillin, comprised a greater percentage of the 120 wound isolates associated with cefazolin prophylaxis than they did of the 95 isolates associated with other prophylactic regimens (25% versus 12.6%, respectively; P < 0.05). In contrast, methicillin-resistant S. aureus isolates were distributed evenly between the two groups (8.3% versus 11.6%, respectively). In vitro assays demonstrated that cefazolin was hydrolyzed faster by BSSA-5 strains than by other beta-lactamase-producing, methicillin-susceptible strains (1.54 versus 0.50 microg/min/10(8) CFU, respectively; P < 0.0001). These data demonstrate that BSSA-5 strains are a distinct subpopulation of methicillin-susceptible S. aureus which frequently cause deep surgical wound infections. Cefazolin use in prophylaxis is a risk factor for BSSA-5 infection.
Kernodle
D S
DS
Department of Medicine, Vanderbilt University School of Medicine, Nashville, Tennessee 37232, USA. kernodds@ctrvax.vanderbilt.edu
Classen
D C
DC
Stratton
C W
CW
Kaiser
A B
AB
D002437:Cefazolin / Q000378:metabolism; D004352:Drug Resistance, Microbial; D008826:Microbial Sensitivity Tests; D010068:Oxacillin / Q000494:pharmacology*; D010406:Penicillins / Q000494:pharmacology*; D013211:Staphylococcus aureus / Q000187:drug effects*; D013530:Surgical Wound Infection / Q000382:microbiology*
D010406:Penicillins; D002437:Cefazolin; D010068:Oxacillin