A preliminary analysis of antineoplastic activity of parvovirus MVMp NS-1 proteins.
Human gastric cancer MKN-45 cells were transfected with pULB 3238, a plasmid carrying MVMp NS-1 gene with its original P4 promoter replaced by the glucocorticoid inducible promoter MMTV-LTR. After the integration and expression of NS-1 gene, some of the transfectants died, while others remained alive, but the growth features of survived cells were changed. For further study on the antineoplastic function of parvoviral NS-1 protein in vivo, transgenic mice carrying NS-1 genes were established by conventional method. Among 4 founders, one of them was found to be able to transmit the transgene to around 50% of their offsprings. RT-PCR was performed to indicate the expression of NS-1 gene in transgenic mice and its mRNA appeared in a variety of tissues. The expression of integrated NS-1 gene may correlate with the decreased incidence of tumor induced in vivo by chemical carcinogens.
Shen
X Z
XZ
Shanghai Institute of Digestive Diseases, Ren Ji Hospital, Shanghai Second Medical University, China.
Tsung
H C
HC
Yang
Y W
YW
Yang
Q H
QH
Xiao
S D
SD
D000818:Animals; D000970:Antineoplastic Agents / Q000494:pharmacology; D002455:Cell Division / Q000187:drug effects; D002461:Cell Line, Transformed; D005260:Female; D015967:Gene Expression Regulation, Viral / Q000187:drug effects; D005814:Genes, Viral; D006131:Growth Inhibitors / Q000502:physiology; D006801:Humans; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D008822:Mice, Transgenic; D009374:Neoplasms, Experimental / Q000139:chemically induced / Q000473:pathology* / Q000517:prevention & control*; D014162:Transfection; D014407:Tumor Cells, Cultured; D017361:Viral Nonstructural Proteins / Q000096:biosynthesis / Q000235:genetics / Q000502:physiology*; D015678:Viral Structural Proteins / Q000235:genetics
D000970:Antineoplastic Agents; D006131:Growth Inhibitors; C081367:NS1 protein, minute virus of mice; D017361:Viral Nonstructural Proteins; D015678:Viral Structural Proteins