Isolation and characterisation of tobacco (Nicotiana tabacum) cDNA clones encoding proteins involved in magnesium chelation into protoporphyrin IX.
We have identified cDNA clones encoding the two Mg chelatase subunits CHL I and CHL H from tobacco (Nicotiana tabacum) by screening a cDNA library with homologous cDNA fragments from Arabidopsis thaliana. A full-length Chl I cDNA clone encodes a peptide with 426 amino acids. The entire cDNA sequence encoding 1382 amino acid long CHL H was obtained by extension of a truncated cDNA fragment using the 'rapid amplification of cDNA ends' (RACE) method. Both genes Chl I and Chl H were strongly expressed in young leaves and to a lesser extent in mature leaves. Only traces of both transcripts were found in flowering organs. Southern blot analysis suggests that CHL I is encoded by a single gene and CHL H most likely by several genes.
Kruse
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Institut für Pflanzengenetik und Kulturpflanzenforschung, Gatersleben, Germany.
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Grimm
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D015152:Blotting, Northern; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000302:isolation & purification*; D017343:Genes, Plant; D008190:Lyases / Q000235:genetics* / Q000378:metabolism; D008969:Molecular Sequence Data; D010940:Plant Proteins / Q000235:genetics* / Q000378:metabolism; D010947:Plants, Toxic; D011524:Protoporphyrins / Q000378:metabolism*; D018749:RNA, Plant / Q000302:isolation & purification; D014026:Nicotiana / Q000201:enzymology* / Q000235:genetics*
D018076:DNA, Complementary; D010940:Plant Proteins; D011524:Protoporphyrins; D018749:RNA, Plant; C028025:protoporphyrin IX; D008190:Lyases; C086788:magnesium chelatase