title: Adenoid basal carcinoma of the uterine cervix: detection of integrated human papillomavirus in a rare tumor of putative "reserve cell" origin.
abstract: This study investigated the role of human papillomavirus (HPV) in adenoid basal carcinoma, a rare neoplasm of the uterine cervix. Nine archival paraffin-embedded tumors were analyzed with non-isotopic in situ hybridization (NISH) for HPV types 6, 11, 16, 18, 31, and 33 using digoxigenin-labelled probes. The polymerase chain reaction (PCR) was performed on each of the cases using E6 consensus primers to HPV. A total of 67% of adenoid basal carcinomas harbored the HPV genome with NISH, of which 3 were PCR-positive. Integrated HPV 16 DNA was demonstrated in 4 of the 6 NISH positive cases. Two cases showed integrated HPV 33. HPV DNA was not detected in the three remaining cases. These results show that the integrated high-risk HPV, in particular type 16, is associated with this uncommon cervical tumor.
authors.affiliation: Department of Anatomical Pathology, School of Pathology, University of the Witwatersrand, Johannesburg, South Africa.
authors.forename: W
authors.initials: W
authors.lastname: Grayson
authors.forename: L F
authors.initials: LF
authors.lastname: Taylor
authors.forename: K
authors.initials: K
authors.lastname: Cooper
mesh_terms: D000328:Adult; D000368:Aged; D002277:Carcinoma / Q000473:pathology* / Q000821:virology*; D018196:Carcinoma, Adenosquamous / Q000473:pathology / Q000821:virology; D004279:DNA, Viral / Q000032:analysis; D005260:Female; D006801:Humans; D017403:In Situ Hybridization; D008875:Middle Aged; D027383:Papillomaviridae / Q000302:isolation & purification*; D016133:Polymerase Chain Reaction; D002583:Uterine Cervical Neoplasms / Q000473:pathology* / Q000821:virology*
chemical_list: D004279:DNA, Viral