title: The combination of in situ hybridization and immunohistochemical analysis: an evaluation of Her2/neu expression in paraffin-embedded breast carcinomas and adjacent normal-appearing breast epithelium.
abstract: The combination of in situ hybridization and immunohistochemical techniques can successfully identify viral DNA/RNA in specific subsets of cellular populations. We recently modified this method to evaluate amplification of the oncogene Her2/neu and overexpression of its protein c-erbB-2 in a series of 15 breast carcinomas. This combination allows the simultaneous evaluation of the oncogene and its corresponding protein expression in single cells and specific cellular populations in histologic tissue sections. Double staining demonstrated heterogeneity within breast carcinomas. In addition, both nuclear and cytoplasmic signals were often detected in morphologically normal-appearing adjacent breast epithelium. The ability to view both the oncogene and its corresponding protein in single cells offers a unique look at the biology of c-erbB-2.
authors.affiliation: Department of Pathology, Dartmouth Medical School, Dartmouth Hitchcock Medical Center, Lebanon, New Hampshire 03756, USA.
authors.forename: N
authors.initials: N
authors.lastname: Ratcliffe
authors.forename: W
authors.initials: W
authors.lastname: Wells
authors.forename: K
authors.initials: K
authors.lastname: Wheeler
authors.forename: V
authors.initials: V
authors.lastname: Memoli
mesh_terms: D001940:Breast / Q000378:metabolism*; D001943:Breast Neoplasms / Q000378:metabolism*; D018567:Breast Neoplasms, Male / Q000378:metabolism*; D018270:Carcinoma, Ductal, Breast / Q000378:metabolism; D002291:Carcinoma, Papillary / Q000378:metabolism; D018734:Genes, erbB-2 / Q000235:genetics*; D006801:Humans; D007150:Immunohistochemistry; D017404:In Situ Hybridization, Fluorescence; D008297:Male; D016612:Paraffin Embedding; D018719:Erb-b2 Receptor Tyrosine Kinases / Q000378:metabolism*; D016707:Tissue Fixation
chemical_list: D018719:Erb-b2 Receptor Tyrosine Kinases