[HPLC of oligodeoxyribonucleoside phosphorothioates ].
Conditions are described for the separation of synthetic oligodeoxyribonucleoside phosphorothioates by HPLC using a SCOUT WP PEI weak anion exchanger and for the separation of oligodeoxyribonucleotides with mixed phosphate and phosphorothioate internucleotide bonds on a Partisphere C18 support in ion-pair conditions. The influence of the nucleoside composition and the number of phosphorothioate bonds on the retention time was studied.
Metelev
V N
VN
Tashlitskił
V N
VN
Agraval
S
S
D002851:Chromatography, High Pressure Liquid / Q000379:methods; D007475:Ion Exchange Resins; D009838:Oligodeoxyribonucleotides / Q000737:chemistry / Q000302:isolation & purification*; D013872:Thionucleosides / Q000737:chemistry / Q000302:isolation & purification*
D007475:Ion Exchange Resins; D009838:Oligodeoxyribonucleotides; D013872:Thionucleosides