title: Immunochemical evidence for increased formation of advanced glycation end products and inhibition by aminoguanidine in diabetic rat lenses.
abstract: Water-soluble and urea-soluble protein fractions from control and streptozotocin-diabetic rats were analyzed for AGEs with a CML-specific monoclonal anti-AGE antibody and a polyclonal anti-AGE antibody. AGEs, CML in particular, were significantly increased in the diabetic rats whereas aminoguandine treatment resulted in significant decrease in AGEs. The data also confirm that CML, a glycoxidation product, is a major epitope of AGE structures in lenses.
authors.affiliation: Department of Biochemistry, Kumamoto University School of Medicine, Japan.
authors.forename: K
authors.initials: K
authors.lastname: Matsumoto
authors.forename: K
authors.initials: K
authors.lastname: Ikeda
authors.forename: S
authors.initials: S
authors.lastname: Horiuchi
authors.forename: H
authors.initials: H
authors.lastname: Zhao
authors.forename: E C
authors.initials: EC
authors.lastname: Abraham
mesh_terms: D000818:Animals; D000918:Antibody Specificity; D003921:Diabetes Mellitus, Experimental / Q000378:metabolism*; D004797:Enzyme-Linked Immunosorbent Assay; D017127:Glycation End Products, Advanced / Q000037:antagonists & inhibitors* / Q000276:immunology; D006146:Guanidines / Q000494:pharmacology*; D007908:Lens, Crystalline / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley; D013311:Streptozocin
chemical_list: D017127:Glycation End Products, Advanced; D006146:Guanidines; D013311:Streptozocin; C004479:pimagedine