Preeclampsia selectively impairs endothelium-dependent relaxation and leads to oscillatory activity in small omental arteries.
The vascular pathophysiology of preeclampsia, a hypertensive disorder unique to human pregnancy, has been postulated to be due to endothelial dysfunction, primarily manifest as deficient nitric oxide (NO) synthesis. We evaluated contraction (KCl and arginine vasopressin [AVP]) and dilation (acetylcholine and bradykinin) in small resistance-size omental arteries obtained during surgery from women with preeclampsia, postulating that these vessels would exhibit augmented contraction and diminished endothelium-dependent relaxation, most likely due to decreased NO synthesis. For comparison, vessels were also obtained from normotensive gravidas, pregnant women with chronic hypertension, or with chronic hypertension and superimposed preeclampsia, as well as from premenopausal nonpregnant controls. Vessels of approximately 200 micron in internal diameter were studied in vitro using a Mulvany-Halpern myograph. Maximal contraction due to either KCl or AVP was significantly augmented in vessels from women with preeclampsia; these vessels all exhibited endothelium- and cyclooxygenase-dependent phasic oscillations while vessels from all other groups exhibited only tonic contractions. Acetylcholine and bradykinin both led to dose- and endothelium-dependent relaxation which was unaffected by inhibitors of NO synthesis. Responses to bradykinin were similar in vessels from normal pregnant and preeclamptic women while those to acetylcholine were absent in vessels from women with preeclampsia. These data suggest specific defects in resistance-artery endothelium from women with preeclampsia.
Pascoal
I F
IF
Department of Obstetrics and Gynecology, Division of Biological Sciences, University of Chicago, Chicago, Illinois 60637, USA.
Lindheimer
M D
MD
Nalbantian-Brandt
C
C
Umans
J G
JG
D000109:Acetylcholine / Q000494:pharmacology; D000328:Adult; D001127:Arginine Vasopressin / Q000494:pharmacology; D001158:Arteries / Q000503:physiopathology; D001920:Bradykinin / Q000494:pharmacology; D004730:Endothelium, Vascular / Q000502:physiology*; D005260:Female; D006801:Humans; D009569:Nitric Oxide / Q000502:physiology; D019335:Nitroarginine / Q000494:pharmacology; D009852:Omentum / Q000098:blood supply; D011225:Pre-Eclampsia / Q000503:physiopathology*; D011247:Pregnancy; D014664:Vasodilation / Q000187:drug effects
D001127:Arginine Vasopressin; D019335:Nitroarginine; D009569:Nitric Oxide; D000109:Acetylcholine; D001920:Bradykinin