Tropism of human herpesvirus 8 for peripheral blood lymphocytes in patients with Castleman's disease.
Multicentric Castleman's disease (MCD), also called multicentric angiofollicular lymphoid hyperplasia, is a systemic lymphoproliferative disorder causing fever, lymphadenopathy and splenomegaly. Recently, Kaposi's sarcoma-associated herpesvirus/human herpesvirus 8 (KSHV/HHV-8) DNA sequences have been detected in cases of MCD. We examined HHV-8 DNA sequences in the peripheral blood mononuclear cells (PBMCs) of two HIV-negative patients with MCD and in PBMCs and the lymph node of a HIV-negative patient with localized Castleman's disease (LCD) by the polymerase chain reaction. The novel sequences were detected in all DNA samples. Furthermore, the sequences were detected in only the CD19+ B-lymphocyte fraction of the patient with LCD as previously reported. However, the sequences were detected in CD19+ B-lymphocyte and CD2+ T-lymphocyte fractions of two patients with MCD. These results suggest that HHV-8 has tropisms for both B lymphocytes and T lymphocytes in Castleman's disease.
Kikuta
H
H
Department of Paediatrics, Hokkaido University School of Medicine, Sapporo, Japan.
Itakura
O
O
Taneichi
K
K
Kohno
M
M
D015139:Blotting, Southern; D005871:Castleman Disease / Q000235:genetics / Q000821:virology*; D006566:Herpesviridae Infections / Q000150:complications* / Q000235:genetics; D019288:Herpesvirus 8, Human / Q000302:isolation & purification*; D006801:Humans; D017354:Point Mutation; D016133:Polymerase Chain Reaction; D013601:T-Lymphocytes / Q000821:virology*