Flow cytometric quantitation of phagocytosis in heparinized complete blood with latex particles and Candida albicans.
We report a rapid method for the flow cytometric quantitation of phagocytosis in heparinized complete peripheral blood (HCPB), using commercially available phycoerythrin-conjugated latex particles of 1 micron diameter. The method is faster and shows greater reproducibility than Bjerknes' (1984) standard technique using propidium iodide-stained Candida albicans, conventionally applied to the leukocytic layer of peripheral blood but here modified for HCPB. We also report a modification of Bjerknes' Intracellular Killing Test to allow its application to HCPB.
Egido
J M
JM
Department of Microbiology and Inmunology, Santiago University, Spain.
Viñuelas
J
J
D002176:Candida albicans / Q000276:immunology*; D005434:Flow Cytometry / Q000379:methods* / Q000706:statistics & numerical data; D006493:Heparin; D006801:Humans; D007202:Indicators and Reagents; D007840:Latex; D009504:Neutrophils / Q000276:immunology*; D009895:Opsonin Proteins / Q000276:immunology; D010316:Particle Size; D010587:Phagocytosis; D011419:Propidium; D015203:Reproducibility of Results; D013997:Time Factors
D007202:Indicators and Reagents; D007840:Latex; D009895:Opsonin Proteins; D011419:Propidium; D006493:Heparin