Preimplantation diagnosis of single gene disorders by two-step oocyte genetic analysis using first and second polar body.
Previous work on preimplantation genetic diagnosis (PGD) of single gene disorders by the first polar body (IPB) analysis has demonstrated that the genotype of a considerable number of embryos resulting from heterozygous oocytes cannot be predicted without testing their second PB (IIPB). To overcome this limitation we introduce a two-step DNA analysis of oocytes using both IPB and IIPB to identify hemizygous mutation-free oocytes following the second meiotic division. In the application of the approach to PGD of cystic fibrosis (CF) Delta F-508 mutation, sickle cell disease, and hemophilia B, 80 oocytes were studied by both PBs, resulting in the identification and transfer of 32 homozygous normal embryos. A follow-up genotyping of 52 embryos, resulting from oocytes tested by both IPB and IIPB demonstrated the accuracy of the predicted genotypes. In addition to a nested PCR analysis of the mutant genes in PBs and resulting embryos, simultaneous amplification of different polymorphic markers was performed, demonstrating the reliability of the two-step polar body analysis of oocytes.
Verlinsky
Y
Y
Reproductive Genetics Institute, Illinois Masonic Medical Center, Chicago 60657, USA.
Rechitsky
S
S
Cieslak
J
J
Ivakhnenko
V
V
Wolf
G
G
Lifchez
A
A
Kaplan
B
B
Moise
J
J
Walle
J
J
White
M
M
Ginsberg
N
N
Strom
C
C
Kuliev
A
A
D000483:Alleles; D004247:DNA / Q000032:analysis; D004622:Embryo, Mammalian / Q000737:chemistry; D005260:Female; D005307:Fertilization in Vitro; D005500:Follow-Up Studies; D030342:Genetic Diseases, Inborn / Q000175:diagnosis* / Q000235:genetics* / Q000517:prevention & control; D005838:Genotype; D006801:Humans; D008540:Meiosis / Q000235:genetics; D009865:Oocytes / Q000737:chemistry*; D016133:Polymerase Chain Reaction; D011237:Predictive Value of Tests; D019836:Preimplantation Diagnosis / Q000379:methods*
D004247:DNA