Independent mechanisms for macrophage binding and macrophage phagocytosis of damaged erythrocytes. Evidence of receptor cooperativity.
The binding and phagocytosis of oxidatively damaged red blood cells (OxRBCs) by mouse peritoneal macrophages can be inhibited by oxidatively modified LDL (OxLDL), implying some commonality at their receptor-binding domains. Studies from many different laboratories support the view that OxRBC binding is due to the disruption of plasma membrane phospholipid asymmetry and the subsequent exposure of phosphatidylserine (PS) on the outer membrane leaflet. Presumably, oxidation of LDL creates a surface structure on it in some way homologous to the PS-rich domain on OxRBCs. Apoptotic cells in some instances are also recognized because of PS exposure on the outer leaflet of the membrane, and apoptotic cells are a common feature of atherosclerotic lesions. In the present studies, the mechanisms of binding and internalization of cells recognized by virtue of their membrane PS were studied using OxRBCs or vanadate-treated erythrocytes (VaRBCs) as models. Disruption of phospholipid asymmetry with vanadate produced cells that were bound by macrophages in the same divalent cation-dependent manner as OxRBCs. However, whereas OxRBCs were rapidly phagocytosed, VaRBCs were not. Stimulation of mouse macrophages with phorbol myristate acetate resulted in a concentration-dependent induction of phagocytosis of bound VaRBCs, an effect that could be prevented by the protein kinase C inhibitor staurosporine. Because phagocytosis of OxRBCs occurred unassisted, we speculated that there must be additional membrane changes induced by oxidation (over and above the disruption of phospholipid asymmetry) that contribute to phagocytosis of OxRBCs, possibly resulting in the ligation of a distinct receptor that does not necessarily contribute to adherence. This proposal is supported by the finding that ligation of macrophage Fc gamma receptors by the anti-Fc gamma RII/RIII antibody 2.4G2 triggers the phagocytosis of bound VaRBCs. Phagocytosis is also triggered by subthreshold opsonization of VaRBC, i.e., by antibody concentrations that do not by themselves cause binding and phagocytosis of native RBCs. Finally, treatment with low concentrations of glutaraldehyde, which causes membrane protein cross-linking, promotes the phagocytosis of VaRBCs, but, at the low concentration used, has little or no effect on binding and phagocytosis of native RBCs. We suggest that the internalization of damaged cells, bound because of PS exposure, requires the cooperation of a PS-binding receptor with at least one additional receptor to trigger an intracellular signaling pathway to initiate phagocytosis.
Sambrano
G R
GR
Department of Medicine, University of California San Diego, La Jolla 92093-0682, USA.
Terpstra
V
V
Steinberg
D
D
D000818:Animals; D002413:Cations, Divalent; D002460:Cell Line; D003300:Copper / Q000737:chemistry; D010450:Endopeptidases / Q000494:pharmacology; D004905:Erythrocyte Aging; D004912:Erythrocytes / Q000502:physiology*; D005976:Glutaral / Q000737:chemistry; D006801:Humans; D008077:Lipoproteins, LDL / Q000378:metabolism; D017737:Macrophages, Peritoneal / Q000502:physiology*; D008565:Membrane Proteins / Q000502:physiology; D051379:Mice; D009895:Opsonin Proteins; D010084:Oxidation-Reduction; D010587:Phagocytosis; D011956:Receptors, Cell Surface / Q000502:physiology*; D014639:Vanadium / Q000737:chemistry
D002413:Cations, Divalent; D008077:Lipoproteins, LDL; D008565:Membrane Proteins; D009895:Opsonin Proteins; D011956:Receptors, Cell Surface; C099252:oxidized low density lipoprotein; D014639:Vanadium; D003300:Copper; D010450:Endopeptidases; D005976:Glutaral