title: Detection of exogenous hydrocortisone in horse urine by gas chromatography-combustion-carbon isotope ratio mass spectrometry.
abstract: A gas chromatography-combustion-isotope ratio mass spectrometry method for confirmation of hydrocortisone abuse in horseracing and equine sports is proposed. Urinary hydrocortisone was converted to a bismethylenedioxy derivative which presents good gas chromatographic properties and brings an extra carbon contribution of only two carbon atoms. Synthetic hydrocortisone has a different 13C abundance from that of natural urinary horse hydrocortisone and the difference is significant, therefore exogenous and endogenous hydrocortisone can be distinguished.
authors.affiliation: Service Central d'Analyse, CNRS, Vernaison, France.
authors.forename: R
authors.initials: R
authors.lastname: Aguilera
authors.forename: M
authors.initials: M
authors.lastname: Becchi
authors.forename: L
authors.initials: L
authors.lastname: Mateus
authors.forename: M A
authors.initials: MA
authors.lastname: Popot
authors.forename: Y
authors.initials: Y
authors.lastname: Bonnaire
authors.forename: H
authors.initials: H
authors.lastname: Casabianca
authors.forename: C K
authors.initials: CK
authors.lastname: Hatton
mesh_terms: D000818:Animals; D000893:Anti-Inflammatory Agents / Q000737:chemistry / Q000652:urine*; D002247:Carbon Isotopes; D004300:Doping in Sports; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods*; D006736:Horses / Q000378:metabolism / Q000652:urine*; D006854:Hydrocortisone / Q000737:chemistry / Q000652:urine*
chemical_list: D000893:Anti-Inflammatory Agents; D002247:Carbon Isotopes; D006854:Hydrocortisone