CpG-oligodeoxynucleotide supports growth of IL-6-dependent 7TD1 murine hybridoma cells.
The mouse-mouse hybridoma plasma cell line 7TD1 requires exogenous IL-6 for growth, and has been used to characterize and assay IL-6. Oligodeoxynucleotides containing CpG (CpG-ODN) and bacterial DNA are immunostimulatory, preventing B-cell apoptosis and inducing cytokine synthesis. We report that the phosphorothioate CpG-ODN 5'-ATAATCGACGTTCAAGCAAG-3' (half maximal effect at 0.3 microg/ml) supports the growth of 7TD1 cells in the absence of added IL-6. A non-CpG control ODN was without effect. No IL-6 production by 7TD1 cells incubated with CpG-ODN was detected by sensitive immunoassay. CpG-ODN-supported growth was not influenced by IL-6-neutralizing antibody, but was completely abolished by chloroquine and quinacrine, agents which inhibit CpG-ODN responses in other cells. Our results show that CpG-ODN can substitute for IL-6 in this cell line. This cell line may be useful for assaying CpG-ODN activity and for screening congeners of chloroquine and quinacrine for their ability to inhibit CpG effects.
Manzel
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Department of Medicine, VA Medical Center, and University of Iowa, Iowa City 52242, USA.
Macfarlane
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D000818:Animals; D000962:Antimalarials / Q000494:pharmacology; D002452:Cell Count / Q000187:drug effects; D002455:Cell Division / Q000187:drug effects; D002738:Chloroquine / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D006825:Hybridomas / Q000166:cytology / Q000187:drug effects*; D015850:Interleukin-6 / Q000494:pharmacology / Q000502:physiology*; D051379:Mice; D009838:Oligodeoxyribonucleotides / Q000494:pharmacology*; D011796:Quinacrine / Q000494:pharmacology
D000962:Antimalarials; D015850:Interleukin-6; D009838:Oligodeoxyribonucleotides; D002738:Chloroquine; D011796:Quinacrine