[A human recombinant erythropoietin produced in human lymphoblastoid cells].
A human recombinant erythropoietin is produced by transfection of a human EBV immortalised lymphoblastoid cell line by human erythropoietin gene. This cell line was selected because it is eucaryotic, human, able to grow in suspension and neither clonogenic nor tumorigenic. Purification of erythropoietin from culture supernatants of lymphoblastoid cells was described and structural, biochemical and functional characteristics were investigated in several studies. Results obtained for this kind of recombinant erythropoietin were similar to those obtained regarding the protein expressed in CHO cells suggesting that both molecules have the same efficiency in clinical applications.
Bounemra-Ben Ghanem
A
A
Service d'Immunologie Cellulaire, C.N.T.S. Tunis, Tunisie.
Cherif
W
W
Lambin
P
P
Jenhani
F
F
Boukef
K
K
D000740:Anemia / Q000188:drug therapy; D002460:Cell Line; D004921:Erythropoietin / Q000494:pharmacology* / Q000627:therapeutic use; D006801:Humans; D011994:Recombinant Proteins
D011994:Recombinant Proteins; D004921:Erythropoietin