Expression of p53 in glioblastoma multiforme cells: relationship to survival, proliferation and glycosylation.
Immunohistochemically assessed p53 expression was correlated with overall survival in 20 patients with glioblastoma multiforme. Various epitopes of p53 molecule were detected using DO-1, DO-7, CM-1 and BP-12-53 antibodies, proliferative activity was estimated using anti-PCNA and anti-Ki-67 antibodies and assessment of glycosylation changes using lectin histochemistry completed the study. The results show a statistically significant correlation between DO-1 binding and survival. However, we failed to find any similar statistically significant relationship using other antibodies used in this study. Nor the intensity of proliferative activity show any correlation with either p53 expression or overall survival. Glycosylation in glioblastoma multiforme cells reflected unspecific changes in tumor cells regardless of any relationship to p53 expression. The findings do not provide clear evidence for the predictive value of p53 expression. On the other hand, relatively larger numbers of p53 immunonegative cases may indicate that other mechanisms are involved in the development of this tumor.
Ehrmann
J
J
Department of Pathology, Faculty of Medicine, Palacký University Olomouc.
Kolár
Z
Z
Kala
M
M
Vojtĕsek
B
B
Komenda
S
S
D000328:Adult; D000368:Aged; D001932:Brain Neoplasms / Q000737:chemistry* / Q000378:metabolism / Q000401:mortality / Q000473:pathology; D002455:Cell Division; D005260:Female; D005909:Glioblastoma / Q000737:chemistry* / Q000378:metabolism / Q000401:mortality / Q000473:pathology; D006031:Glycosylation; D006801:Humans; D019394:Ki-67 Antigen / Q000032:analysis; D008297:Male; D008875:Middle Aged; D018809:Proliferating Cell Nuclear Antigen / Q000032:analysis; D015996:Survival Rate; D016159:Tumor Suppressor Protein p53 / Q000032:analysis*
D019394:Ki-67 Antigen; D018809:Proliferating Cell Nuclear Antigen; D016159:Tumor Suppressor Protein p53