The differential transforming activities of Tal1 oncoproteins.
TAL1 (Or SCL) oncogene produces both full-length pp42 and truncated pp22 phosphoproteins in T-cell acute lymphoblastic leukemia cells. To investigate the transforming properties of these two oncoproteins, permanent transfected NIH3T3 cell lines were generated that constitutively express either pp42 or pp22. Both cell lines exhibited similar morphology as parental NIH3T3 cells. However, they showed differential anchorage independent growth in soft-agar. The clonogenicity of pp22 expressing cells was ten folds higher than that of pp42 expressing cells. This result might implicate the differential transformation potential of the two Tal1 oncoproteins.
Hsu
S F
SF
Institute of Life Sciences, National Sun Yat-Sen University, Kaohsiung, Taiwan, Republic of China.
Cheng
J T
JT
D016475:3T3 Cells / Q000166:cytology / Q000187:drug effects; D000818:Animals; D051792:Basic Helix-Loop-Helix Proteins; D002448:Cell Adhesion / Q000187:drug effects; D002455:Cell Division / Q000187:drug effects; D002471:Cell Transformation, Neoplastic; D004268:DNA-Binding Proteins / Q000235:genetics / Q000502:physiology*; D051379:Mice; D011518:Proto-Oncogene Proteins; D000073942:T-Cell Acute Lymphocytic Leukemia Protein 1; D014157:Transcription Factors; D014162:Transfection
D051792:Basic Helix-Loop-Helix Proteins; D004268:DNA-Binding Proteins; D011518:Proto-Oncogene Proteins; D000073942:T-Cell Acute Lymphocytic Leukemia Protein 1; D014157:Transcription Factors; C489366:Tal1 protein, mouse