Lung carcinoma imaging using a synthetic laminin derivative radioiodinated peptide YIGSR.
The synthetic laminin pentapeptide tyrosyl-isoleucyl-glycyl-seryl-arginine (YIGSR) binds to a metastasis associated high-affinity laminin receptor. The aim of this study was to investigate if the radiolabeled peptide can be considered as a basis for a potential tumor-imaging radiopharmaceutical.


METHODS
Iodine-131-labeled YIGSR was injected in mice inoculated with Lewis Lung carcinoma, as well as in normal controls. The experimental animals were imaged on a gamma camera 10 hr after peptide administration. The same peptide was also labeled with 125I and administered to tumor-bearing and normal mice. At various time-points after peptide administration, the experimental animals were killed, and the radioactivity in the tumor, lung, liver and spleen was measured. Microscopic autoradiography was performed in histological sections of the same tissues.


RESULTS
The tumor and the spleen of tumor-bearing animals were imaged on a gamma camera. No significant blood-pool background was detected. No other organ except urinary bladder and thyroid was imaged in normal animals. The peptide was retained on tumor and spleen of tumor-bearing animals. Twenty-four hours after peptide administration, the tumor, lung, liver and spleen of animals with tumors contained significantly more radioactivity than the same tissues of equally treated normal controls. The radiolabeled peptide YIGSR was detected by microscopic autoradiography on the surface of certain tumor cells, but not on the surface of any normal cell.


CONCLUSIONS
Although extensive research is still required, the peptide YIGSR can be considered as a basis for the development of a receptor specific radiopharmaceutical useful for the in vivo estimation of the metastatic potential of tumors. This radiopharmaceutical may be helpful in staging and prognostic-related decisions on cancer treatment.
Koliakos
G
G
Department of Biological Chemistry, AHEPA University Hospital, Aristotle University of Thessaloniki, Greece.
Trontzos
C
C
Kouzi-Koliakos
K
K
Kanellaki
M
M
Grammaticos
P
P
D000818:Animals; D001345:Autoradiography; D018827:Carcinoma, Lewis Lung / Q000000981:diagnostic imaging* / Q000473:pathology / Q000556:secondary; D007457:Iodine Radioisotopes; D007797:Laminin; D008168:Lung / Q000473:pathology; D008175:Lung Neoplasms / Q000000981:diagnostic imaging* / Q000473:pathology / Q000556:secondary; D051379:Mice; D008810:Mice, Inbred C57BL; D009368:Neoplasm Transplantation; D009842:Oligopeptides; D011877:Radionuclide Imaging; D019275:Radiopharmaceuticals
D007457:Iodine Radioisotopes; D007797:Laminin; D009842:Oligopeptides; D019275:Radiopharmaceuticals; C081571:tyrosyl-isoleucyl-glycyl-seryl-arginine