Preparation of (R)-N-methylsalsolinol via reversed-phase high-performance liquid chromatography using beta-cyclodextrin as a mobile-phase additive.
A new preparation method was devised using beta-cyclodextrin as a mobile-phase additive in a reversed-phase high-performance liquid chromatography system. Preparative separation of the biologically active (R)-enantiomer was achieved from racemic N-methylsalsolinol. Beta-cyclodextrin was removed completely in good yield by acid extraction and solid-phase extraction. By a slight modification, this method will be applicable to the isolation of various types of biologically important enantiomers.
Minami
C
C
Department of Applied Chemistry, Nagoya Institute of Technology, Japan.
Deng
Y
Y
Urano
F
F
Kaiya
T
T
Kohda
K
K
Kawai
M
M
Naoi
M
M
D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D002942:Circular Dichroism; D003505:Cyclodextrins / Q000737:chemistry*; D012490:Salsoline Alkaloids / Q000737:chemistry / Q000302:isolation & purification*; D013056:Spectrophotometry, Ultraviolet; D013237:Stereoisomerism; D044005:Tetrahydroisoquinolines
D003505:Cyclodextrins; D012490:Salsoline Alkaloids; D044005:Tetrahydroisoquinolines; C032837:salsoline