Subunit characterization of the Caenorhabditis elegans chaperonin containing TCP-1 and expression pattern of the gene encoding CCT-1.
The chaperonin containing TCP-1 (CCT) from the free-living nematode Caenorhabditis elegans was purified and shown to contain at least seven subunit species ranging from 52-65 kDa. SDS-gel electrophoresis and Western blot analyses with antibodies against C. elegans CCT-1 and CCT-5 and an antibody which recognizes a conserved region in vertebrate CCT subunits confirm that the subunit compositions of CCTs from distantly related organisms (C. elegans and bovine species) are remarkably similar. Surprisingly, the co-purified HSP60 chaperonin present in the C. elegans CCT preparation has the greatest binding activity for denatured actin. Expression of a reporter gene under the control of the C. elegans cct-1 promoter is found to be mainly restricted to neuronal and muscle tissues, an observation which is consistent with the participation of CCT in actin and tubulin folding.
Leroux
M R
MR
Department of Biochemistry and Molecular Biology, University of British Columbia, Vancouver, Canada.
Candido
E P
EP
D000595:Amino Acid Sequence; D000818:Animals; D019677:Artificial Gene Fusion; D017173:Caenorhabditis elegans; D029742:Caenorhabditis elegans Proteins; D002417:Cattle; D056404:Chaperonin Containing TCP-1; D018833:Chaperonins / Q000737:chemistry* / Q000235:genetics; D004587:Electrophoresis, Agar Gel; D005786:Gene Expression Regulation; D017238:Genes, Helminth; D056385:Group II Chaperonins; D006360:Heat-Shock Proteins / Q000737:chemistry* / Q000235:genetics* / Q000302:isolation & purification / Q000378:metabolism; D015801:Helminth Proteins / Q000737:chemistry / Q000235:genetics* / Q000302:isolation & purification; D008840:Microfilament Proteins / Q000378:metabolism; D018832:Molecular Chaperones / Q000737:chemistry* / Q000235:genetics* / Q000302:isolation & purification / Q000378:metabolism; D008969:Molecular Sequence Data; D011401:Promoter Regions, Genetic; D019076:Transgenes; D001616:beta-Galactosidase / Q000235:genetics
D029742:Caenorhabditis elegans Proteins; D006360:Heat-Shock Proteins; D015801:Helminth Proteins; D008840:Microfilament Proteins; D018832:Molecular Chaperones; C491569:cct-1 protein, C elegans; D001616:beta-Galactosidase; D056404:Chaperonin Containing TCP-1; D018833:Chaperonins; D056385:Group II Chaperonins