Chemiluminescent assays for choline and phospholipase-D using a flow injection system.
A flow injection chemiluminescent method is described for the determination of choline. The method is based on the production of hydrogen peroxide from choline using on-line covalently bound immobilized choline oxidase column. The product is mixed downstream and detected via the cobalt catalyzed chemiluminescent oxidation of luminol. The detection limit is 1 x 10(-7) mol/L, with rsd 1.8 to 2.8% in the range 2-10 x 10(-5) mol/L. The sample throughput is 30 per hour. The method was applied to the determination of choline produced off-line from phosphatidylcholine using phospholipase-D isolated from cabbage.
Yaqoob
M
M
Department of Chemistry, University of Balochistan, Quetta, Pakistan.
Nabi
A
A
Masoom-Yasinzai
M
M
D000429:Alcohol Oxidoreductases; D002138:Calibration; D002794:Choline / Q000032:analysis*; D004563:Electrochemistry / Q000295:instrumentation / Q000379:methods; D004800:Enzymes, Immobilized; D006861:Hydrogen Peroxide / Q000032:analysis; D008163:Luminescent Measurements; D010739:Phospholipase D / Q000032:analysis*
D004800:Enzymes, Immobilized; D006861:Hydrogen Peroxide; D000429:Alcohol Oxidoreductases; C019888:choline oxidase; D010739:Phospholipase D; D002794:Choline