title: EDEN and EDEN-BP, a cis element and an associated factor that mediate sequence-specific mRNA deadenylation in Xenopus embryos.
abstract: During Xenopus early development, gene expression is regulated mainly at the translational level by the length of the poly(A) tail of mRNAs. The Eg family and c-mos maternal mRNAs are deadenylated rapidly and translationally repressed after fertilization. Here, we characterize a short sequence element (EDEN) responsible for the rapid deadenylation of Eg5 mRNA. Determining the core EDEN sequence permitted us to localize the c-mos EDEN sequence. The c-mos EDEN confered a rapid deadenylation to a reporter gene. The EDEN-specific RNA-binding protein (EDEN-BP) was purified and a cDNA obtained. EDEN-BP is highly homologous to a human protein possibly involved in myotonic dystrophy. Immunodepleting EDEN-BP from an egg extract totally abolished the EDEN-mediated deadenylation activity, but did not affect the default deadenylation activity. Therefore, EDEN-BP constitutes the first trans-acting factor for which an essential role in the specificity of mRNA deadenylation has been directly demonstrated.
authors.affiliation: CNRS UPR 41, Université de Rennes 1, Campus de Beaulieu, 35042 Rennes cedex, France.
authors.forename: L
authors.initials: L
authors.lastname: Paillard
authors.forename: F
authors.initials: F
authors.lastname: Omilli
authors.forename: V
authors.initials: V
authors.lastname: Legagneux
authors.forename: T
authors.initials: T
authors.lastname: Bassez
authors.forename: D
authors.initials: D
authors.lastname: Maniey
authors.forename: H B
authors.initials: HB
authors.lastname: Osborne
mesh_terms: D000225:Adenine / Q000378:metabolism*; D000595:Amino Acid Sequence; D000818:Animals; D001665:Binding Sites; D003001:Cloning, Molecular; D008969:Molecular Sequence Data; D016296:Mutagenesis; D009154:Mutation; D011485:Protein Binding; D017231:Proto-Oncogene Proteins c-mos / Q000235:genetics / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics* / Q000378:metabolism; D016601:RNA-Binding Proteins / Q000235:genetics* / Q000378:metabolism; D017384:Sequence Deletion; D016159:Tumor Suppressor Protein p53 / Q000378:metabolism; D014981:Xenopus / Q000196:embryology / Q000235:genetics*; D029867:Xenopus Proteins
chemical_list: C110462:EDEN-specific RNA-binding protein, Xenopus; D012333:RNA, Messenger; D016601:RNA-Binding Proteins; D016159:Tumor Suppressor Protein p53; D029867:Xenopus Proteins; D017231:Proto-Oncogene Proteins c-mos; D000225:Adenine