title: Expression of a gene encoding a ribosomal p40 protein and identification of an active promoter site.
abstract: The promoter of a gene encoding a ribosome-associated protein of 40 kDa from Arabidopsis thaliana (A-p40) was sequenced and the expression of the gene studied. A-p40 was expressed in the same organs and with the same variations as the eukaryotic elongation factor 1 alpha (eEF1A), another gene coding for a protein involved in translation Arabidopsis plants transformed with a beta-glucuronidase (GUS) gene driven by the A-p40 promoter confirm that A-p40 is expressed in actively dividing and growing cells. eEF1A promoter-GUS fusions have the same pattern of expression. Comparison of cis-acting elements from A-p40 and eEF1A revealed some common elements. A-p40 promoter deletions and transient gene expression in transfected Arabidopsis protopasts allowed the identification of trap40, a cis-acting element regulating gene expression. Gel retardation experiments indicate that eEF1A and A-p40 are regulated by different cis-acting elements. The role of such elements is discussed.
authors.affiliation: UMR 5546 Centre National de la Recherche Scientifique-Université Paul Sabatier, Toulouse, France.
authors.forename: I
authors.initials: I
authors.lastname: Scheer
authors.forename: M D
authors.initials: MD
authors.lastname: Ludevid
authors.forename: F
authors.initials: F
authors.lastname: Regad
authors.forename: B
authors.initials: B
authors.lastname: Lescure
authors.forename: R F
authors.initials: RF
authors.lastname: Pont-Lezica
mesh_terms: D017360:Arabidopsis / Q000235:genetics*; D001483:Base Sequence; D018506:Gene Expression Regulation, Plant; D008969:Molecular Sequence Data; D009928:Organ Specificity / Q000235:genetics; D010940:Plant Proteins / Q000096:biosynthesis / Q000235:genetics*; D011401:Promoter Regions, Genetic; D012269:Ribosomal Proteins / Q000096:biosynthesis / Q000235:genetics*; D017422:Sequence Analysis, DNA; D017384:Sequence Deletion
chemical_list: D010940:Plant Proteins; D012269:Ribosomal Proteins; C086117:Rpsa protein, rat