Effects of the bile salt sodium deoxycholate, protamine, and inflammatory mediators on the potassium permeability of the frog nerve perineurium.
An electrophysiological method was used to measure the potassium permeability (PK) of the perineurium of the sciatic nerve of frogs Rana temporaria and R. pipiens. Isolated but intact nerves were mounted in a grease-gap chamber, and compound action potential and DC potential monitored. Change in the DC potential (delta DC) in response to challenge with 100 mM [K+] Ringer was used to assess the K+ permeability of the perineurium, since change in DC potential under these conditions reflected changes in the axonal resting potential. The permeability of the perineurium was calculated from the published calibration curve relating delta DC to bathing [K+] in desheathed nerves of Abbott et al. (1997). In the control condition, PK was < 1.1 x 10(-6) cm.s-1. The bile salt sodium deoxycholate (DOC, 1-4 mM) caused a dose-dependent increase in PK, which reached a maximum of 1.7 x 10(-5) cm.s-1 after 2-min exposure to 4 mM DOC, but access of K+ to the endoneurial compartment was more restricted after DOC than after desheathing. Protamine phosphate (1 mM) and protamine sulphate (0.1-5 mg/ml equals 0.125-6.25 mM) had no effect on PK. Neither histamine (0.4-40 mg/ml), bradykinin (0.1-5 mg/ml) nor serotonin (5-hydroxytryptamine, 0.1-5 mg/ml) affected PK. The frog nerve perineurium appears to be relatively insensitive to chemical agents and inflammatory mediators, in contrast to the endothelial cells forming the endoneurial blood-nerve barrier and the blood-brain barrier.
Todd
B A
BA
Department of Clinical Neurological Sciences, Southampton University Hospital, UK. b.todd@swan.ac.uk
Sedgwick
E M
EM
Abbott
N J
NJ
D000200:Action Potentials / Q000187:drug effects / Q000502:physiology; D000818:Animals; D001647:Bile Acids and Salts / Q000494:pharmacology; D002199:Capillary Permeability / Q000187:drug effects; D003840:Deoxycholic Acid / Q000494:pharmacology*; D004594:Electrophysiology; D018836:Inflammation Mediators / Q000494:pharmacology*; D010525:Peripheral Nerves / Q000187:drug effects / Q000378:metabolism*; D010710:Phosphates / Q000494:pharmacology; D011188:Potassium / Q000493:pharmacokinetics*; D011479:Protamines / Q000494:pharmacology*; D011894:Rana pipiens; D011896:Rana temporaria; D012584:Sciatic Nerve / Q000648:ultrastructure
D001647:Bile Acids and Salts; D018836:Inflammation Mediators; D010710:Phosphates; D011479:Protamines; D003840:Deoxycholic Acid; D011188:Potassium