Comparison of CO2 generation (BACTEC) and viable-count methods to determine the postantibiotic effect of antimycobacterial agents against Mycobacterium avium complex.
The postantibiotic effects (PAEs) of antimycobacterial agents determined with a BACTEC TB-460 instrument (CO2 production) and by a traditional viable-count method against Mycobacterium avium complex (MAC) were not significantly different (P > 0.05). The longest PAEs following a 2-h exposure to 2x the MIC were induced by amikacin (10.3 h), rifampin (9.7 h), and rifabutin (9.5 h), while the shortest PAEs resulted from clofazimine (1.7 h) and ethambutol (1.1 h) exposure. CO2 generation is a valid and efficient means of determining in vitro PAEs against MAC.
Zhanel
G G
GG
Department of Medical Microbiology and Faculty of Pharmacy, University of Manitoba, Health Sciences Centre, Winnipeg, Canada. ggzhanel@pcs.mb.ca
Saunders
M H
MH
Wolfe
J N
JN
Hoban
D J
DJ
Karlowsky
J A
JA
Kabani
A M
AM
D000583:Amikacin / Q000494:pharmacology; D000995:Antitubercular Agents / Q000494:pharmacology*; D002245:Carbon Dioxide / Q000378:metabolism*; D015169:Colony Count, Microbial; D004977:Ethambutol / Q000494:pharmacology; D008826:Microbial Sensitivity Tests; D015269:Mycobacterium avium Complex / Q000187:drug effects* / Q000378:metabolism / Q000502:physiology; D017828:Rifabutin / Q000494:pharmacology; D012293:Rifampin / Q000494:pharmacology
D000995:Antitubercular Agents; D002245:Carbon Dioxide; D017828:Rifabutin; D000583:Amikacin; D004977:Ethambutol; D012293:Rifampin