[Changes in tissue and blood polyamine levels following chemotherapy in rats with urinary bladder carcinoma induced by N-butyl-N-(4-hydroxybutyl) nitosamine in rats].
BACKGROUND
Polyamines are recognized as cell growth factors. We attempted to determine whether alterations in the levels of tissue and blood polyamines were useful biochemical makers for monitoring the efficacy of the chemotherapy for bladder tumors.


METHODS
The concentrations of three polyamines of diamine, spermidine and spermine in urinary bladder and blood were determined in male F344 rats with urinary bladder carcinoma induced by N-butyl-N-(4-hydroxybutyl) nitrosamine (BBN), following chemotherapy with cisplatin, methotrexate and pirarubicin.


RESULTS
Bladder carcinoma was observed in 5 of 20 rats of the chemotherapeutic group, and 16 of 20 rats of the control group given saline alone. The levels of spermidine, spermine and total polyamine in both bladder and blood of the treated rats were significantly lower than those of the control rats.


CONCLUSIONS
The study suggested that the levels of tissue and blood polyamines could be used as biochemical markers for monitoring the efficacy of the chemotherapy for bladder tumors.
Taniguchi
M
M
Department of Urology, Gifu Prefectural Gifu Hospital.
Minoshima
K
K
Takeuchi
T
T
Sakai
S
S
Deguchi
T
T
Kawada
Y
Y
Sato
K
K
Hara
A
A
D000818:Animals; D000970:Antineoplastic Agents / Q000627:therapeutic use*; D002085:Butylhydroxybutylnitrosamine; D002273:Carcinogens; D003959:Diamines / Q000097:blood; D008297:Male; D011073:Polyamines / Q000097:blood / Q000378:metabolism*; D051381:Rats; D011916:Rats, Inbred F344; D013095:Spermidine / Q000097:blood; D013096:Spermine / Q000097:blood; D001749:Urinary Bladder Neoplasms / Q000139:chemically induced / Q000188:drug therapy / Q000378:metabolism*
D000970:Antineoplastic Agents; D002273:Carcinogens; D003959:Diamines; D011073:Polyamines; D013096:Spermine; D002085:Butylhydroxybutylnitrosamine; D013095:Spermidine