A new model of experimental allergic rhinitis using Japanese cedar pollen in guinea pigs.
In the majority of the models of experimental allergic rhinitis, antigen challenge has been performed by single topical instillation or perfusion with the solution. The present study was performed to establish a good model using Japanese cedar pollen, which is able to repeatedly induce allergy restricted to the upper airway. Guinea pigs sensitized with the pollen extracts were subjected to quantitative and repeated inhaling of the pollen with a devised apparatus. Following the respective challenges, the nasal cavity was washed with a new technique: Washing with physiologic saline was performed from one nostril to the other one, the latter of which was kept under slightly reduced pressure. When the animal was subjected to cedar pollen inhalation, almost all the pollens inhaled were located in the upper airway. At the 5th inhalation, nasal cavity lavage revealed that both albumin leakage and histamine release into the nasal cavity were increased at maximum levels in 1 hr (respectively 2 mg and 3 ng/animal); and at the same time, a considerable number of leukocytes, especially eosinophils, were found migrating into the nasal cavity for at least 10 hr. The present methods can permit various analyses of allergic rhinitis and the assessment of drugs without sacrificing the animal over the long term.
Nabe
T
T
Department of Pharmacology, Kyoto Pharmaceutical University, Yamashina, Japan.
Shimizu
K
K
Mizutani
N
N
Saeki
Y
Y
Yamamura
H
H
Takenaka
H
H
Kohno
S
S
D000418:Albumins / Q000378:metabolism; D000536:Aluminum Hydroxide / Q000494:pharmacology; D000818:Animals; D006168:Guinea Pigs; D006632:Histamine / Q000378:metabolism; D006636:Histamine Release / Q000187:drug effects; D007958:Leukocyte Count; D008297:Male; D009296:Nasal Cavity / Q000166:cytology / Q000378:metabolism / Q000473:pathology*; D010323:Passive Cutaneous Anaphylaxis; D011058:Pollen / Q000276:immunology*; D006255:Rhinitis, Allergic, Seasonal / Q000378:metabolism / Q000473:pathology*; D013997:Time Factors
D000418:Albumins; D000536:Aluminum Hydroxide; D006632:Histamine