title: Mutational analysis of the potato virus Y 5' untranslated region for alteration in translational enhancement in tobacco protoplasts.
abstract: The 185 nucleotide 5' untranslated region (5'UTR) of potato virus Y ordinary strain (PVY-O) showed translation-enhancing activity on the beta-glucuronidase (GUS) gene in tobacco protoplasts. Mutational analysis of the 5'UTR was done to find sequence motifs necessary for the enhancement. Deletions within the 1-130 nucleotide region of 5'UTR stimulated the GUS expression in some cases, while the GUS activity declined with deletions in the 131-185 nucleotide region. The results indicated that the last 55 nucleotides of PVY-O 5'UTR might play the much important role in the translational enhancement in plant cells.
authors.affiliation: Department of Biotechnology, Graduate School of Agricultural and Life Sciences, University of Tokyo, Japan.
authors.forename: L J
authors.initials: LJ
authors.lastname: Yang
authors.forename: M
authors.initials: M
authors.lastname: Hidaka
authors.forename: J
authors.initials: J
authors.lastname: Sonoda
authors.forename: H
authors.initials: H
authors.lastname: Masaki
authors.forename: T
authors.initials: T
authors.lastname: Uozumi
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D015967:Gene Expression Regulation, Viral; D005966:Glucuronidase / Q000235:genetics*; D008969:Molecular Sequence Data; D009154:Mutation; D010947:Plants, Toxic; D017800:Potyvirus / Q000201:enzymology / Q000235:genetics*; D014176:Protein Biosynthesis; D011523:Protoplasts / Q000378:metabolism; D016415:Sequence Alignment; D014026:Nicotiana / Q000235:genetics
chemical_list: D005966:Glucuronidase