Electrically induced vasomotor responses and their propagation in rat renal vessels in vivo.
1. Vasomotor responses (VMR) induced by local electrical stimulation were studied in the vasculature of the split hydronephrotic rat kidney by in vivo microscopy. 2. Unipolar pulses, which were applied by a micropipette positioned close to the vessel wall, elicited local and propagated VMR. Depolarizing and hyperpolarizing currents caused vasoconstriction and vasodilatation, respectively. 3. The magnitude of VMR could be controlled within seconds by variation of pulse frequency, pulse width and voltage. VMR were abolished by slight retraction of the stimulating micropipette. Repetitive electrical stimulation resulted in reproducibly uniform VMR. 4. Propagated VMR decayed with increasing distance from the stimulation site. They decayed more rapidly in the upstream than in the downstream flow direction in interlobular arteries. The longitudinal decay was well approximated by an exponential function with significantly different length constants of 150 +/- 40 microns (upstream, n = 5) and 420 +/- 90 microns (downstream, n = 8). 5. Our results show that vasomotor responses, which are initiated by changes in membrane potential, are propagated over distances of potential physiological importance in interlobular arteries.
Steinhausen
M
M
Institut für Anatomie und Zellbiologie I, Universität Heidelberg, Germany. michael.steinhausen@urz.uni-heidelberg.de
Endlich
K
K
Nobiling
R
R
Parekh
N
N
Schütt
F
F
D000818:Animals; D001160:Arterioles / Q000294:innervation / Q000502:physiology / Q000503:physiopathology*; D004558:Electric Stimulation; D005260:Female; D006869:Hydronephrosis / Q000503:physiopathology*; D008564:Membrane Potentials; D009131:Muscle, Smooth, Vascular / Q000294:innervation / Q000502:physiology / Q000503:physiopathology; D051381:Rats; D017208:Rats, Wistar; D012077:Renal Artery / Q000294:innervation / Q000502:physiology / Q000503:physiopathology*; D014661:Vasoconstriction / Q000502:physiology*; D014664:Vasodilation / Q000502:physiology*; D014666:Vasomotor System / Q000502:physiology / Q000503:physiopathology*; D014741:Video Recording