title: Effect of administration of galactosamine hydrochloride on rat liver mitochondria.
abstract: The effect of galactosamine on liver mitochondrial functions was studied in vivo in rats at 12hr, 24hr and 36hr after the administration of the drug. State 3 respiration decreased significantly with both NAD+ linked and FAD linked substrates. Respiratory control ratio, an index of membrane integrity and P/O ratio which is a measure of phosphorylation efficiency decreased significantly. There was a significant decrease in the activities of NADH dehydrogenase, succinate dehydrogenase and cytochrome oxidase. A significant decrease was also seen on membrane potential, cytochrome aa3, cytochrome b, cytochrome c and on phospholipids of mitochondria. The observed mitochondrial dysfunctions were related to increased lipid peroxidation, which could cause loss of membrane integrity and a decreased rate of phosphorylation. It is proposed that increased lipid peroxidation was responsible for the inhibition on both oxidation and phosphorylation in mitochondria in galactosamine treated rats.
authors.affiliation: Department of Biochemistry, School of Life Sciences, University of Hyderabad, India.
authors.forename: P
authors.initials: P
authors.lastname: Padma
authors.forename: O H
authors.initials: OH
authors.lastname: Setty
mesh_terms: D000818:Animals; D019069:Cell Respiration / Q000187:drug effects; D003580:Cytochromes / Q000032:analysis; D004579:Electron Transport / Q000187:drug effects; D003576:Electron Transport Complex IV / Q000378:metabolism; D005688:Galactosamine / Q000494:pharmacology*; D008054:Lipid Peroxides / Q000032:analysis; D008055:Lipids / Q000032:analysis; D008930:Mitochondria, Liver / Q000187:drug effects* / Q000378:metabolism; D009245:NADH Dehydrogenase / Q000378:metabolism; D010766:Phosphorylation; D051381:Rats; D013385:Succinate Dehydrogenase / Q000378:metabolism
chemical_list: D003580:Cytochromes; D008054:Lipid Peroxides; D008055:Lipids; D005688:Galactosamine; D013385:Succinate Dehydrogenase; D009245:NADH Dehydrogenase; D003576:Electron Transport Complex IV