Cisplatin-stimulated murine bone marrow-derived macrophages secrete oncostatin M.
Cisplatin (CP), a widely used anticancer drug activates cells of the immune system to a tumoricidal state, and thus functions as a potent biological response modifier. Expression of oncostatin M (OSM), a novel cytokine having a growth regulatory effect, was studied in bone marrow-derived macrophages treated with cisplatin. Supernatants from CP-stimulated macrophages were found to be cytostatic for OSM-sensitive A375 melanoma cells. Immunoblot analysis with anti-OSM antibody revealed that expression of OSM in macrophages upon CP stimulation is a rapid process and within 30 min of CP treatment, a significant amount of OSM is secreted into the culture supernatant. These results therefore indicate that CP can stimulate murine bone marrow-derived macrophages to produce OSM which can be implicated as one of the cytostatic/ cytocidal factors in the antitumour action of cisplatin-stimulated macrophages.
Sodhi
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School of Biotechnology, Banaras Hindu University, Varanasi, India.
Shishodia
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Shrivastava
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D000818:Animals; D000970:Antineoplastic Agents / Q000494:pharmacology*; D001854:Bone Marrow Cells / Q000166:cytology* / Q000187:drug effects; D002470:Cell Survival; D002478:Cells, Cultured; D002945:Cisplatin / Q000494:pharmacology*; D006131:Growth Inhibitors / Q000032:analysis / Q000378:metabolism*; D008163:Luminescent Measurements; D008264:Macrophages / Q000187:drug effects* / Q000378:metabolism*; D051379:Mice; D008807:Mice, Inbred BALB C; D053683:Oncostatin M; D010455:Peptides / Q000032:analysis / Q000378:metabolism*; D014407:Tumor Cells, Cultured
D000970:Antineoplastic Agents; D006131:Growth Inhibitors; C508352:Osm protein, mouse; D010455:Peptides; D053683:Oncostatin M; D002945:Cisplatin