title: Effects of sequential reagent addition on technetium-99m red blood cell labeling efficiency using the UltraTag kit.
abstract: OBJECTIVE
  The purpose of this study was to investigate how the reagent-adding sequence affects 99mTc red blood cell (RBC) labeling efficiency using the UltraTag RBC kit.
  
  
  METHODS
  The package insert for preparation and quality control was followed during the experiment. The reagent-adding sequences were then altered during preparation.
  
  
  RESULTS
  If acid-citrate dextrose is added before the NaOCl, the labeling efficiency decreases dramatically. However, the order of addition of Na 99mTcO4 and acid-citrate dextrose did not affect the labeling efficiency.
  
  
  CONCLUSIONS
  Addition of the NaOCl solution before the acid-citrate dextrose solution is imperative for proper labeling efficiency.
authors.affiliation: Department of Radiology, College of Physicians and Surgeons, Columbia University, New York, New York, USA.
authors.forename: J
authors.initials: J
authors.lastname: Massler
authors.forename: C
authors.initials: C
authors.lastname: Mento
authors.forename: S
authors.initials: S
authors.lastname: Shodavaram
authors.forename: R
authors.initials: R
authors.lastname: Cason
authors.forename: S
authors.initials: S
authors.lastname: Das
authors.forename: T S
authors.initials: TS
authors.lastname: Wang
authors.forename: R L
authors.initials: RL
authors.lastname: Van Heertum
mesh_terms: D004912:Erythrocytes; D006801:Humans; D007553:Isotope Labeling; D011933:Reagent Kits, Diagnostic; D013667:Technetium
chemical_list: D011933:Reagent Kits, Diagnostic; D013667:Technetium