title: [AccBSI - a novel restriction endonuclease from Acinetobacter calcoaceticus BS].
abstract: The recognition site of a new restriction endonuclease from Acinetobacter calcoaceticus BS was determined. This is a nonpalindromic sequence. AccBSI restrictase cleaves DNA chains in the middle of the recognition sequence; therefore, ligation of its digestion fragments restored AccBSI recognition sites and generated palindromic sequences recognized by SacI and SacII restrictases.
authors.forename: M A
authors.initials: MA
authors.lastname: Abdurashitov
authors.forename: E V
authors.initials: EV
authors.lastname: Kiseleva
authors.forename: T V
authors.initials: TV
authors.lastname: Miakisheva
authors.forename: V S
authors.initials: VS
authors.lastname: Dedkov
authors.forename: A V
authors.initials: AV
authors.lastname: Shevchenko
authors.forename: C Kh
authors.initials: CKh
authors.lastname: Degtiarev
mesh_terms: D016954:Acinetobacter calcoaceticus / Q000201:enzymology* / Q000235:genetics; D001665:Binding Sites / Q000235:genetics; D004262:DNA Restriction Enzymes / Q000235:genetics / Q000302:isolation & purification*; D004269:DNA, Bacterial / Q000235:genetics / Q000378:metabolism; D015252:Deoxyribonucleases, Type II Site-Specific / Q000235:genetics / Q000302:isolation & purification*; D006868:Hydrolysis; D013379:Substrate Specificity
chemical_list: D004269:DNA, Bacterial; D004262:DNA Restriction Enzymes; C109672:CCGCTC-specific type II deoxyribonucleases; D015252:Deoxyribonucleases, Type II Site-Specific