In vitro assembly of a ribonucleoprotein particle corresponding to the platform domain of the 30S ribosomal subunit.
A fragment of the 16S RNA of Thermus thermophilus corresponding to the central domain (nucleotides 547-895) has been prepared by transcription in vitro. Incubation of this fragment with the total 30S ribosomal proteins has resulted in the formation of a compact 12S ribonucleoprotein particle. This particle contained five T. thermophilus proteins corresponding to Escherichia coli ribosomal proteins S6, S8, S11, S15, and possibly S18, all of which were previously shown to interact with the central domain of the 16S RNA and to be localized in the platform (side bulge) of the 30S ribosomal subunit. When examined by electron microscopy, isolated particles have an appearance that is similar in size and shape to the corresponding morphological features of the 30S subunit. We conclude that the central domain of the 16S RNA can independently and specifically assemble with a defined subset of ribosomal proteins into a compact ribonucleoprotein particle corresponding to the platform (side bulge) of the 30S subunit.
Agalarov
S C
SC
Institute of Protein Research, Russian Academy of Sciences, Pushchino, Moscow Region, 142292 Russia.
Zheleznyakova
E N
EN
Selivanova
O M
OM
Zheleznaya
L A
LA
Matvienko
N I
NI
Vasiliev
V D
VD
Spirin
A S
AS
D001483:Base Sequence; D004926:Escherichia coli; D008854:Microscopy, Electron; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation; D017510:Protein Folding; D012329:RNA, Bacterial / Q000737:chemistry* / Q000648:ultrastructure; D012335:RNA, Ribosomal / Q000737:chemistry / Q000648:ultrastructure; D012336:RNA, Ribosomal, 16S / Q000737:chemistry* / Q000648:ultrastructure; D012269:Ribosomal Proteins / Q000737:chemistry* / Q000648:ultrastructure; D016963:Thermus thermophilus / Q000235:genetics* / Q000648:ultrastructure; D014158:Transcription, Genetic
D012329:RNA, Bacterial; D012335:RNA, Ribosomal; D012336:RNA, Ribosomal, 16S; C075526:RNA, ribosomal, 12S; D012269:Ribosomal Proteins; C040469:ribosomal protein S30