Cleavage effect of oligoribonucleotides substituted at the cleavage sites with modified pyrimidine- and purine-nucleosides.
The precursor of an RNA molecule from T4-infected E. coli cells (p2Spl RNA) has the capacity to cleave itself at specific positions [UpA (139-140) and CpA (170-171)], within a putative loop and stem structure. This sequence-specific cleavage requires at least a monovalent cation and non-ionic detergents. In order to determine the influence of the pyrimidine and purine bases on these sequence-specific cleavage reactions, we studied the cleavage reactions of hairpin loop RNAs substituted at the cleavage sites with modified pyrimidine- and purine-nucleosides. The cleavage was affected by the 2'-hydroxyl groups and the bases of the pyrimidines, and the 6-amino group of the purine.
Hosono
K
K
Department of Industrial Chemistry, Chiba Institute of Technology, Narashino, Japan.
Gozu
H
H
Hosaka
H
H
Sakamoto
K
K
Yokoyama
S
S
Takai
K
K
Takaku
H
H
D001482:Base Composition; D001483:Base Sequence; D001665:Binding Sites / Q000235:genetics; D009690:Nucleic Acid Conformation; D009843:Oligoribonucleotides / Q000138:chemical synthesis / Q000737:chemistry* / Q000378:metabolism; D011684:Purine Nucleosides / Q000138:chemical synthesis / Q000737:chemistry* / Q000378:metabolism; D011741:Pyrimidine Nucleosides / Q000138:chemical synthesis / Q000737:chemistry* / Q000378:metabolism; D016337:RNA, Catalytic / Q000737:chemistry / Q000235:genetics / Q000378:metabolism
D009843:Oligoribonucleotides; D011684:Purine Nucleosides; D011741:Pyrimidine Nucleosides; D016337:RNA, Catalytic