Establishment and characterization of a canine T-lymphoblastoid cell line derived from malignant lymphoma.
A canine lymphoma cell line (CL-1) was established in culture from tumor cells found in the pleural fluid of a 7-year old female Japanese terrier with thymic form lymphoma. The CL-1 cells were positive for CD45 and MHC class II and negative for CD4, CD5, CD8, Thy-1 and B-cell specific antigen and surface immunoglobulin. The CL-1 cells had a rearranged T-cell receptor beta-chain gene and a germ-line form immunoglobulin gene, indicating that the CL-1 cells represented a monoclonally expanded population of canine alpha beta T-cell lineage.
Momoi
Y
Y
Department of Veterinary Internal Medicine, Faculty of Agriculture, University of Tokyo, Japan.
Okai
Y
Y
Watari
T
T
Goitsuka
R
R
Tsujimoto
H
H
Hasegawa
A
A
D000818:Animals; D015139:Blotting, Southern / Q000662:veterinary; D004283:Dog Diseases / Q000276:immunology / Q000473:pathology*; D004285:Dogs; D005260:Female; D005434:Flow Cytometry / Q000662:veterinary; D015333:Gene Rearrangement, beta-Chain T-Cell Antigen Receptor / Q000235:genetics / Q000276:immunology; D005803:Genes, Immunoglobulin / Q000276:immunology; D000949:Histocompatibility Antigens Class II / Q000276:immunology; D016130:Immunophenotyping / Q000662:veterinary; D017493:Leukocyte Common Antigens / Q000276:immunology; D054198:Precursor Cell Lymphoblastic Leukemia-Lymphoma / Q000276:immunology / Q000473:pathology / Q000662:veterinary*; D016693:Receptors, Antigen, T-Cell, alpha-beta / Q000276:immunology; D013953:Thymus Neoplasms / Q000276:immunology / Q000473:pathology / Q000662:veterinary*; D014407:Tumor Cells, Cultured
D000949:Histocompatibility Antigens Class II; D016693:Receptors, Antigen, T-Cell, alpha-beta; D017493:Leukocyte Common Antigens