title: Modified Evans blue fluorimetry for determination of pulmonary vascular permeability in rats sustaining burns, and delayed fluid resuscitation of burn shock.
abstract: The present experiment used modified Evans blue fluorimetry to determine changes in pulmonary vascular permeability using a delayed resuscitation model of burn shock in rats. The results showed that pulmonary vascular permeability in the immediate resuscitation (IR) group regressed to normal 12-24 h following the burn whereas it regressed slowly in the delayed resuscitation (DR) group. The study showed that Evans blue fluorimetry is a reliable and sensitive method for determining pulmonary vascular permeability, and dimethyl formamide is a preferable extracting solution which provides a quick and convenient means for scientific research.
authors.affiliation: Burn Center, Changhai Hospital, People's Republic of China.
authors.forename: W
authors.initials: W
authors.lastname: Lu
authors.forename: Y
authors.initials: Y
authors.lastname: Chen
authors.forename: Z
authors.initials: Z
authors.lastname: Xia
authors.forename: Z
authors.initials: Z
authors.lastname: Fang
mesh_terms: D000818:Animals; D002056:Burns / Q000175:diagnosis / Q000503:physiopathology* / Q000628:therapy; D002199:Capillary Permeability; D004396:Coloring Agents / Q000493:pharmacokinetics*; D004195:Disease Models, Animal; D005070:Evans Blue / Q000493:pharmacokinetics*; D005470:Fluorometry / Q000379:methods*; D016014:Linear Models; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley; D012016:Reference Values; D015532:Rehydration Solutions / Q000008:administration & dosage*; D012151:Resuscitation / Q000379:methods; D012680:Sensitivity and Specificity; D012774:Shock, Traumatic / Q000175:diagnosis / Q000503:physiopathology* / Q000628:therapy
chemical_list: D004396:Coloring Agents; D015532:Rehydration Solutions; D005070:Evans Blue