title: Secondary structures and starvation-induced frameshifting.
abstract: We have examined the effect of a downstream secondary structure (the stem-loop sequence found downstream on the MMTV gag-pro frameshift site) on frameshifting at a bacterial shifty site (U UUC AUA) that responds strongly to a isoleucine-tRNA limitation. Our findings are as follows: (i) the downstream stem-loop has little effect on frameshifting in growing, unstarved cells; (ii) the stem-loop increases the frameshifting effect of isoleucine-tRNA limitation about fourfold, and this synergism is maximal with a distance of 5-9 nucleotides between the 'hungry' AUA codon and the stem-loop; and (iii) a stem-loop of different sequence at the same position has the same effect.
authors.affiliation: Department of Genetics, University of Washington, Seattle 98195-7360, USA.
authors.forename: J
authors.initials: J
authors.lastname: Atkinson
authors.forename: M
authors.initials: M
authors.lastname: Dodge
authors.forename: J
authors.initials: J
authors.lastname: Gallant
mesh_terms: D004926:Escherichia coli; D018965:Frameshifting, Ribosomal / Q000235:genetics; D015750:Genes, gag; D008324:Mammary Tumor Virus, Mouse / Q000235:genetics; D009690:Nucleic Acid Conformation; D012355:RNA, Transfer, Ile / Q000235:genetics*; D012367:RNA, Viral
chemical_list: D012355:RNA, Transfer, Ile; D012367:RNA, Viral