title: Expression of Lamp-1 and Lamp-2 and their interactions with galectin-3 in human tumor cells.
abstract: Lysosomal-membrane-associated glycoproteins (Lamps) 1 and 2 are rarely found on the plasma membranes of normal cells. There is evidence suggesting an increase in their cell-surface expression in tumor cells, with some data indicating that the adhesion of some cancer cells to the extracellular matrix is partly mediated by interactions between Lamps and E-selectin and between Lamps and galectins (endogenous-galactoside-binding lectins). The present study examined the expression of Lamp-1 and Lamp-2 and their interactions with galectin-3 in different human tumor cell lines. Indirect immunofluorescence staining revealed accumulation of Lamp molecules at the edges of A2058 human metastasizing melanoma cells suggesting that these glycoproteins could participate in cell adhesion. Flow cytometry showed the presence of cell-surface Lamps in A2058, HT1080 (human fibrosarcoma) and CaCo-2 (human colon-adenocarcinoma) cells. Treatment with 2 mM sodium butyrate for 24 and 48 hr resulted in a significant increase in Lamps surface expression. A strong binding of A2058 to recombinant galectin-3 was detected by FACS. The application of 2 and 5 mM butyrate for the same incubation period enhanced galectin-3 binding to Lamps-expressing cells. Our results support the idea that Lamps may be considered a new family of adhesive glycoproteins participating in the complex process of tumor invasion and metastasis.
authors.affiliation: Department of Physiological Chemistry, Facultés Universitaires Notre Dame De la Paix, Medical Faculty, Namur, Belgium.
authors.forename: V
authors.initials: V
authors.lastname: Sarafian
authors.forename: M
authors.initials: M
authors.lastname: Jadot
authors.forename: J M
authors.initials: JM
authors.lastname: Foidart
authors.forename: J J
authors.initials: JJ
authors.lastname: Letesson
authors.forename: F
authors.initials: F
authors.lastname: Van den Brûle
authors.forename: V
authors.initials: V
authors.lastname: Castronovo
authors.forename: R
authors.initials: R
authors.lastname: Wattiaux
authors.forename: S W
authors.initials: SW
authors.lastname: Coninck
mesh_terms: D000230:Adenocarcinoma / Q000378:metabolism; D015703:Antigens, CD / Q000187:drug effects / Q000378:metabolism*; D000943:Antigens, Differentiation / Q000187:drug effects / Q000378:metabolism*; D002822:Choriocarcinoma / Q000378:metabolism; D003110:Colonic Neoplasms / Q000378:metabolism; D005354:Fibrosarcoma / Q000378:metabolism; D005434:Flow Cytometry; D019084:Fluorescent Antibody Technique, Indirect; D037502:Galectin 3; D006801:Humans; D051907:Lysosomal Membrane Proteins; D008545:Melanoma / Q000378:metabolism; D008562:Membrane Glycoproteins / Q000187:drug effects / Q000378:metabolism*; D009363:Neoplasm Proteins / Q000187:drug effects / Q000378:metabolism*; D014407:Tumor Cells, Cultured / Q000187:drug effects / Q000378:metabolism
chemical_list: D015703:Antigens, CD; D000943:Antigens, Differentiation; D037502:Galectin 3; D051907:Lysosomal Membrane Proteins; D008562:Membrane Glycoproteins; D009363:Neoplasm Proteins