title: Regulatory region of expression of Thiobacillus ferrooxidans leuB gene in Escherichia coli.
abstract: The regulatory region of Thiobacillus ferrooxidans leuB gene was analyzed. The deletion analysis indicated that the promoter sequences CATCCG and TATTAT, which were similar to the consensus -35 and -10 sequences of Escherichia coli, respectively, existed. The transcriptional initiation site was located at the position of cytosine -26. The deletion analysis of the upstream region suggested the existence of a regulatory region by leucine and the region related to transcription of the gene.
authors.affiliation: Department of Bioresources Chemistry, Faculty of Agriculture, Okayama University, Japan.
authors.forename: H
authors.initials: H
authors.lastname: Kawaguchi
authors.forename: K
authors.initials: K
authors.lastname: Inagaki
authors.forename: H
authors.initials: H
authors.lastname: Tanaka
mesh_terms: D050539:3-Isopropylmalate Dehydrogenase; D000429:Alcohol Oxidoreductases / Q000235:genetics*; D001483:Base Sequence; D004926:Escherichia coli / Q000235:genetics*; D015964:Gene Expression Regulation, Bacterial / Q000235:genetics*; D005798:Genes, Bacterial; D007930:Leucine / Q000096:biosynthesis; D008969:Molecular Sequence Data; D010957:Plasmids; D011401:Promoter Regions, Genetic / Q000235:genetics; D013855:Thiobacillus / Q000235:genetics*
chemical_list: D000429:Alcohol Oxidoreductases; D050539:3-Isopropylmalate Dehydrogenase; D007930:Leucine