Properties of the P-type ATPases encoded by the copAP operons of Helicobacter pylori and Helicobacter felis.
The cop operons of Helicobacter pylori and Helicobacter felis were cloned by gene library screening. Both operons contain open reading frames for a P-type ion pump (CopA) with homology to Cd2+ and Cu2+ ATPases and a putative ion binding protein (CopP), the latter representing a CopZ homolog of the copYZAB operon of Enterococcus hirae. The predicted CopA ATPases contained an N-terminal GMXCXXC ion binding motif and a membrane-associated CPC sequence. A synthetic N-terminal peptide of the H. pylori CopA ATPase bound to Cu2+ specifically, and gene disruption mutagenesis of CopA resulted in an enhanced growth sensitivity of H. pylori to Cu2+ but not to other divalent cations. As determined experimentally, H. pylori CopA contains four pairs of transmembrane segments (H1 to H8), with the ATP binding and phosphorylation domains lying between H6 and H7, as found for another putative transition metal pump of H. pylori (K. Melchers, T. Weitzenegger, A. Buhmann, W. Steinhilber, G. Sachs, and K. P. Schäfer, J. Biol. Chem. 271:446-457, 1996). The corresponding transmembrane segments of the H. felis CopA pump were identified by hydrophobicity analysis and via sequence similarity. To define functional domains, similarly oriented regions of the two enzymes were examined for sequence identity. Regions with high degrees of identity included the N-terminal Cu2+ binding domain, the regions of ATP binding and phosphorylation in the energy transduction domain, and a transport domain consisting of the last six transmembrane segments with conserved cysteines in H4, H6, and H7. The data suggest that H. pylori and H. felis employ conserved mechanisms of ATPase-dependent copper resistance.
Bayle
D
D
University of California-Los Angeles and Wadsworth Veterans Affairs Medical Center, USA.
Wängler
S
S
Weitzenegger
T
T
Steinhilber
W
W
Volz
J
J
Przybylski
M
M
Schäfer
K P
KP
Sachs
G
G
Melchers
K
K
D000251:Adenosine Triphosphatases / Q000235:genetics / Q000378:metabolism*; D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000235:genetics*; D015139:Blotting, Southern; D002462:Cell Membrane / Q000378:metabolism; D004269:DNA, Bacterial; D016998:Helicobacter / Q000201:enzymology / Q000235:genetics*; D008670:Metals / Q000378:metabolism; D008969:Molecular Sequence Data; D009876:Operon; D017386:Sequence Homology, Amino Acid; D013045:Species Specificity
D001426:Bacterial Proteins; C071263:CopA protein, Bacteria; D004269:DNA, Bacterial; D008670:Metals; D000251:Adenosine Triphosphatases