Functional analyses of mammalian protein kinase C isozymes in budding yeast and mammalian fibroblasts.
BACKGROUND
The PKC1 gene of Saccharomyces cerevisiae encodes a homologue of mammalian protein kinase C (PKC) that is required for yeast cell growth. Pkc1 has been proposed to regulate a protein kinase cascade which includes the Bck1, Mkk1/Mkk2 and Mpk1 kinases. The functional relationship between Pkc1 and mammalian PKCs is unknown. Another signal transduction in Saccharomyces cerevisiae, the mating pheromone signalling pathway, is mediated by a heterotrimeric G protein, and causes cell cycle arrest in the G1 interval. It is not clear whether PKC is involved in this pathway. The effects of overexpression of PKCs in mammalian cells have been widely studied to analyse the function of PKCs in vivo.


RESULTS
We isolated a human cDNA which encodes a protein kinase C type eta (PKC-eta) by complementation of pkc1 mutations in Saccharomyces cerevisiae. The human PKC-eta was able to complement the growth defect caused by the deletion of PKC1, whereas PKC-eta was unable to suppress the defect caused by deletion of BCK1. We also isolated human cDNAs that can suppress the adaptation defect of sst2. One of them encodes a protein kinase C type delta (PKC-delta). Expression of this gene in yeast stimulated an adaptation to the pheromone response. Human PKC-delta suppressed the adaptation defect of a pheromone receptor mutation lacking its C-terminal domain, but not that of a G protein beta-subunit mutation eliminating signal-induced phosphorylation, and not the lethality of the gpa1 null mutation. Moreover, overexpression of PKC-eta in NIH3T3 cells induced anchorage-independent growth.


CONCLUSIONS
PKC-eta has a biological activity which is closely related to Pkc1, and PKC-eta activates the Pkc1-mediated pathway through an activation of the Bck1 kinase that is a homologue of MAP kinase kinase kinase. PKC-eta appears to play a critical role in growth control of yeast and mammalian cells. Suppression experiments with PKC-delta suggest that PKC-delta desensitizes the pathway by regulating an aspect of G protein function.
Nomoto
S
S
Laboratory for Genes of Motor Systems, Bio-Mimetic Control Research Program, RIKEN, Nagoya, Japan.
Watanabe
Y
Y
Ninomiya-Tsuji
J
J
Yang
L X
LX
Nagai
Y
Y
Kiuchi
K
K
Hagiwara
M
M
Hidaka
H
H
Matsumoto
K
K
Irie
K
K
D000220:Adaptation, Biological; D003001:Cloning, Molecular; D005347:Fibroblasts; D005656:Fungal Proteins / Q000235:genetics; D005816:Genetic Complementation Test; D006801:Humans; D007527:Isoenzymes / Q000235:genetics / Q000378:metabolism*; D000072235:Mating Factor; D020929:Mitogen-Activated Protein Kinase Kinases; D010455:Peptides / Q000494:pharmacology; D010675:Pheromones / Q000494:pharmacology; D011493:Protein Kinase C / Q000235:genetics / Q000378:metabolism*; D051745:Protein Kinase C-delta; D011494:Protein Kinases / Q000235:genetics; D011994:Recombinant Proteins / Q000096:biosynthesis; D012441:Saccharomyces cerevisiae; D029701:Saccharomyces cerevisiae Proteins; D015398:Signal Transduction; D013045:Species Specificity; D013489:Suppression, Genetic
D005656:Fungal Proteins; D007527:Isoenzymes; D010455:Peptides; D010675:Pheromones; D011994:Recombinant Proteins; D029701:Saccharomyces cerevisiae Proteins; D000072235:Mating Factor; D011494:Protein Kinases; C097993:Pkc1 protein, Trichoderma reesei; C081975:protein kinase C eta; C072075:BCK1 protein, S cerevisiae; C497258:PRKCD protein, human; D011493:Protein Kinase C; D051745:Protein Kinase C-delta; D020929:Mitogen-Activated Protein Kinase Kinases