C-erbB-2 amplification and expression in gestational trophoblastic disease correlates with DNA content and karyotype.
The subject of this retrospective study was to evaluate the potential benefit of the c-erbB-2 oncogene amplification and expression in 27 complete hydatidiform moles as well as in 9 cases of persistent gestational trophoblastic disease defined by elevated serum beta-human choriongonadotropin. The persistent cases were histopathologically classified as 5 complete hydatidiform moles, 3 invasive moles and 1 choriocarcinoma. In addition, we determined the DNA content and the karyotype of the sex chromosomes. The data were correlated with the histopathologic characteristics of gestational trophoblastic diseases. Cases with c-erbB-2 amplification and expression in combination with DNA hyperploidy showed higher proliferation and a more aggressive behavior (2 complete hydatidiform moles with lung and liver metastases, 2 invasive moles and 1 choriocarcinoma). XY karyotype was evident in the choriocarcinoma and in two complete hydatidiform moles with advanced stage and DNA hyperploidy.
Bauer
M
M
Department of Gynecopathology, University of Vienna, Austria.
Horn
L C
LC
Kowalzik
J
J
Mair
W
W
Czerwenka
K
K
D004247:DNA / Q000032:analysis; D005260:Female; D015870:Gene Expression; D018734:Genes, erbB-2 / Q000235:genetics; D006801:Humans; D006828:Hydatidiform Mole / Q000235:genetics / Q000378:metabolism; D007091:Image Processing, Computer-Assisted; D007150:Immunohistochemistry; D017404:In Situ Hybridization, Fluorescence; D007621:Karyotyping; D011003:Ploidies; D011247:Pregnancy; D018719:Erb-b2 Receptor Tyrosine Kinases / Q000378:metabolism*; D012189:Retrospective Studies; D014328:Trophoblastic Neoplasms / Q000235:genetics* / Q000378:metabolism*; D014594:Uterine Neoplasms / Q000235:genetics* / Q000378:metabolism*; D014960:X Chromosome; D014998:Y Chromosome
D004247:DNA; D018719:Erb-b2 Receptor Tyrosine Kinases