Tumor cytogenetics revisited: comparative genomic hybridization and spectral karyotyping.
Fluorescence in situ hybridization techniques allow the visualization and localization of DNA target sequences on the chromosomal and cellular level and have evolved as exceedingly valuable tools in basic chromosome research and cytogenetic diagnostics. Recent advances in molecular cytogenetic approaches, namely comparative genomic hybridization and spectral karyotyping, now allow tumor genomes to be surveyed for chromosomal aberrations in a single experiment and permit identification of tumor-specific chromosomal aberrations with unprecedented accuracy. Comparative genomic hybridization utilizes the hybridization of differentially labeled tumor and reference DNA to generate a map of DNA copy number changes in tumor genomes. Comparative genomic hybridization is an ideal tool for analyzing chromosomal imbalances in archived tumor material and for examining possible correlations between these findings and tumor phenotypes. Spectral karyotyping is based on the simultaneous hybridization of differentially labeled chromosome painting probes (24 in human), followed by spectral imaging that allows the unique display of all human (and other species) chromosomes in different colors. Spectral karyotyping greatly facilitates the characterization of numerical and structural chromosomal aberrations, therefore improving karyotype analysis considerably. We review these new molecular cytogenetic concepts, describe applications of comparative genomic hybridization and spectral karyotyping for the visualization of chromosomal aberrations as they relate to human malignancies and animal models thereof, and provide evidence that fluorescence in situ hybridization has developed as a robust and reliable technique which justifies its translation to cytogenetic diagnostics.
Ried
T
T
National Human Genome Research Institute, National Institutes of Health, Bethesda, MD 20892-4479, USA.
Liyanage
M
M
du Manoir
S
S
Heselmeyer
K
K
Auer
G
G
Macville
M
M
Schröck
E
E
D000818:Animals; D003582:Cytogenetics / Q000379:methods*; D006801:Humans; D007621:Karyotyping / Q000379:methods*; D009369:Neoplasms / Q000235:genetics* / Q000473:pathology*; D009374:Neoplasms, Experimental / Q000235:genetics / Q000473:pathology; D009693:Nucleic Acid Hybridization