A potential new gene (tccA) on IncP plasmid RK2 and transposon Tn1721: relationship of its product to the TrwC relaxase/helicase of IncW plasmid R388.
Computational analysis of the fully sequenced 60-kb genome of broad-host-range IncP alpha plasmid RK2 revealed a previously unreported potential protein-coding sequence, an 80-codon open reading frame (tccA), located in the region between the vegetative origin of replication (oriV) and the tetR gene of the tetracycline resistance determinant. The coding region is also present in the transposon Tn1721 tet region, which is nearly identical to the tet region of RK2. Remarkably, the predicted polypeptide product of the coding region displays 56% identity and 72% similarity with the C-terminal domain of the TrwC relaxase/helicase protein of IncW plasmid R388.
Jovanovic
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Department of Microbiology, College of Physicians and Surgeons, Columbia University, New York, New York 10032. USA.
Figurski
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D000595:Amino Acid Sequence; D001426:Bacterial Proteins; D002352:Carrier Proteins / Q000235:genetics* / Q000378:metabolism; D004254:DNA Nucleotidyltransferases / Q000235:genetics*; D004251:DNA Transposable Elements; D019426:Integrases; D008969:Molecular Sequence Data; D011815:R Factors; D045522:Recombinases
D001426:Bacterial Proteins; D002352:Carrier Proteins; D004251:DNA Transposable Elements; D045522:Recombinases; C110589:tccA protein, IncP plasmid RK2; D004254:DNA Nucleotidyltransferases; D019426:Integrases; C115006:integron integrase IntI1