Localization of 14-3-3 proteins in the nuclei of arabidopsis and maize.
It has been demonstrated that 14-3-3 proteins are present in the nuclei of Arabidopsis thaliana and Zea mays cells using laser scanning confocal microscopy and immunocytochemistry with monoclonal antibodies against plant 14-3-3 proteins. Confirmation of nuclear localization provides insight into the range of functions normally attributed to 14-3-3 proteins, especially since the association of 14-3-3s with transcription factors is (thus far) a phenomenon unique to plants, and since 14-3-3 proteins do not possess a recognizable nuclear targeting sequence.
Bihn
E A
EA
Horticultural Sciences Department, University of Florida, Gainesville 32611, USA.
Paul
A L
AL
Wang
S W
SW
Erdos
G W
GW
Ferl
R J
RJ
D048948:14-3-3 Proteins; D017360:Arabidopsis / Q000648:ultrastructure*; D002467:Cell Nucleus / Q000648:ultrastructure; D002478:Cells, Cultured; D007150:Immunohistochemistry; D018613:Microscopy, Confocal; D008854:Microscopy, Electron; D018517:Plant Roots; D011506:Proteins / Q000032:analysis*; D011523:Protoplasts; D014446:Tyrosine 3-Monooxygenase; D003313:Zea mays / Q000648:ultrastructure*
D048948:14-3-3 Proteins; D011506:Proteins; D014446:Tyrosine 3-Monooxygenase