Single chain observation on collapse transition in giant DNA induced by negatively-charged polymer.
Poly-glutamic acid, a polyanion, has been shown to induce the all-or-none collapse transition in individual duplex DNAs from an elongated coil into a collapsed compact state, through the observation of single chains by fluorescence microscopy. This experimental finding is discussed in relation to the change in the higher-order structure of DNAs in the cytoplasmic space induced by a change in the concentration of polyanions, such as mRNA, as a possible route for feedback in gene expression.
Ichiba
Y
Y
Division of Informatics for Natural Sciences, Graduate School of Human Informatics, Nagoya University, Japan.
Yoshikawa
K
K
D017122:Bacteriophage T4 / Q000737:chemistry; D004247:DNA / Q000378:metabolism* / Q000648:ultrastructure; D004279:DNA, Viral / Q000737:chemistry / Q000378:metabolism; D005786:Gene Expression Regulation / Q000235:genetics; D007091:Image Processing, Computer-Assisted; D008856:Microscopy, Fluorescence; D009690:Nucleic Acid Conformation; D010316:Particle Size; D000071228:Polyelectrolytes; D011092:Polyethylene Glycols / Q000378:metabolism; D011099:Polyglutamic Acid / Q000378:metabolism; D011108:Polymers / Q000494:pharmacology*; D012333:RNA, Messenger / Q000494:pharmacology
D004247:DNA; D004279:DNA, Viral; D000071228:Polyelectrolytes; D011092:Polyethylene Glycols; D011099:Polyglutamic Acid; D011108:Polymers; D012333:RNA, Messenger