title: DNA sequence variation within vanA, vanB, vanC-1, and vanC-2/3 genes of clinical Enterococcus isolates.
abstract: We studied the DNA sequence variation of van genes of 34 isolates of Enterococcus spp. The isolates containing the vanB gene exhibited between 0 and 41 base pair changes per 801 bp studied when the vanB sequences were compared to that of the reference strain Enterococcus faecalis V583. The isolates carrying the vanC-2 gene exhibited between 0 and 23 base pair changes per 346 bp studied when the vanC-2 sequences were compared to that of the reference strain E. casseliflavus ATCC 25788. Little variation was noted in the vanA and vanC-1 genes.
authors.affiliation: Division of Clinical Microbiology, Mayo Clinic and Foundation, Rochester, Minessota 55902, USA. patel.robin@mayo.edu
authors.forename: R
authors.initials: R
authors.lastname: Patel
authors.forename: J R
authors.initials: JR
authors.lastname: Uhl
authors.forename: P
authors.initials: P
authors.lastname: Kohner
authors.forename: M K
authors.initials: MK
authors.lastname: Hopkins
authors.forename: J M
authors.initials: JM
authors.lastname: Steckelberg
authors.forename: B
authors.initials: B
authors.lastname: Kline
authors.forename: F R
authors.initials: FR
authors.lastname: Cockerill
mesh_terms: D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000235:genetics*; D001483:Base Sequence; D019729:Carbon-Oxygen Ligases / Q000235:genetics*; D016983:Enterococcus / Q000235:genetics*; D014644:Genetic Variation; D006801:Humans; D008969:Molecular Sequence Data; D010453:Peptide Synthases / Q000235:genetics*; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D012689:Sequence Homology, Nucleic Acid
chemical_list: D001426:Bacterial Proteins; C071437:VanA ligase, Bacteria; C079913:VanB protein, Enterococcus; C079914:VanC protein, Bacteria; D019729:Carbon-Oxygen Ligases; D010453:Peptide Synthases; C108395:VanC protein, Enterococcus