title: Molecular cloning and physical mapping of the daptomycin gene cluster from Streptomyces roseosporus.
abstract: The daptomycin biosynthetic gene cluster of Streptomyces roseosporus was analyzed by Tn5099 mutagenesis, molecular cloning, partial DNA sequencing, and insertional mutagenesis with cloned segments of DNA. The daptomycin biosynthetic gene cluster spans at least 50 kb and is located about 400 to 500 kb from one end of the approximately 7,100-kb linear chromosome. We identified two peptide synthetase coding regions interrupted by a 10- to 20-kb region that may encode other functions in lipopeptide biosynthesis.
authors.affiliation: Lilly Research Laboratories, A Division of Eli Lilly and Company, Indianapolis, Indiana 46285, USA.
authors.forename: M A
authors.initials: MA
authors.lastname: Mchenney
authors.forename: T J
authors.initials: TJ
authors.lastname: Hosted
authors.forename: B S
authors.initials: BS
authors.lastname: Dehoff
authors.forename: P R
authors.initials: PR
authors.lastname: Rosteck
authors.forename: R H
authors.initials: RH
authors.lastname: Baltz
mesh_terms: D001426:Bacterial Proteins / Q000235:genetics*; D002874:Chromosome Mapping / Q000379:methods*; D003001:Cloning, Molecular; D017576:Daptomycin / Q000096:biosynthesis*; D005798:Genes, Bacterial / Q000235:genetics; D005810:Multigene Family / Q000235:genetics*; D016254:Mutagenesis, Insertional; D010453:Peptide Synthases / Q000235:genetics*; D015183:Restriction Mapping; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D013302:Streptomyces / Q000201:enzymology / Q000235:genetics* / Q000378:metabolism
chemical_list: D001426:Bacterial Proteins; C109892:CpsA protein, Streptomyces roseosporus; C109893:cpsB protein, Streptomyces roseosporus; D010453:Peptide Synthases; D017576:Daptomycin