Simple one-step procedure for the separation of apolipoproteins A1 and A2 by high-performance liquid chromatography.
A simple assay method for apolipoproteins apo A1 and apo A2 by HPLC is introduced. The simple one-step method is based on fractionation of apo A1 and apo A2 from other serum proteins which are precipitated at 100 degrees C and removed by centrifugation. The apo A1 and apo A2 which remain in solution can be recovered and resolved by size-exclusion chromatography without ultracentrifugation and delipidation by an organic solvent. This makes sample preparation easier. The recoveries of apo A1 and apo A2 were 104.26% and 101.04%; the precision (C.V.%) of apo A1 and apo A2 was 0.88 and 1.63 respectively.
Xiong
Z
Z
Central Laboratory of Affiliated Hospital, Shandong Medical University, Jinan, China.
D016632:Apolipoprotein A-I / Q000302:isolation & purification*; D016633:Apolipoprotein A-II / Q000302:isolation & purification*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D006801:Humans; D015203:Reproducibility of Results; D013997:Time Factors
D016632:Apolipoprotein A-I; D016633:Apolipoprotein A-II