pH is regulated differently by glucose in skeletal muscle from fed and starved rats: a study using 31P-NMR spectroscopy.
The aim of this study was to determine whether exogenous glucose metabolism influences the pH in superfused EDL muscle from growing rats fed or starved for 48 h (body weight 55 and 45 g, respectively). Energy state and intracellular pH of muscle were repeatedly monitored by 31P-nuclear magnetic resonance spectroscopy (31P-NMRS); glycogen and other energy metabolites were assayed enzymatically in muscle extracts at the end of the experiment. In EDL muscles from starved rats superfused with glucose for 4 h, intracellular pH was elevated (7-7.3), lactate concentration low, glycogen repletion very intense and citrate synthase activity high. We conclude that glucose was routed mainly toward both oxidative phosphorylation and glycogen synthesis in EDL muscles after food deprivation of rats. In contrast, the major pathway in muscles from fed rats may be glycolysis because the glycogen pool remained constant throughout the experiment. The additional and minor pH component (in the range of 6.5 to 6.8) seen in muscles from fed rats, even in the presence of exogenous glucose, might be due to impaired glucose utilization because this component appears also in muscles from starved rats superfused without glucose or with a nonmetabolizable analog of glucose. Consequently, direct pH measurement by 31P-NMR may be considered to be a precise criterion for evaluation of differences in metabolic potentialities of muscle studied ex vivo in relation to the nutritional state of rats.
Meynial-Denis
D
D
Unité d'Etude du Metabolisme Azoté et Centre de Recherches en Nutrition Humaine d'Auvergne, INRA Theix Centre de Recherches de Clermont-Ferrand-Theix-63122-Ceyrat, France.
Mignon
M
M
Foucat
L
L
Bielicki
G
G
Ouali
A
A
Tassy
C
C
Renou
J P
JP
Grizard
J
J
Arnal
M
M
D000255:Adenosine Triphosphate / Q000378:metabolism; D000818:Animals; D003847:Deoxyglucose / Q000378:metabolism; D004734:Energy Metabolism; D005260:Female; D005508:Food Deprivation / Q000502:physiology*; D005947:Glucose / Q000008:administration & dosage / Q000378:metabolism* / Q000494:pharmacology; D006003:Glycogen / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D009682:Magnetic Resonance Spectroscopy; D018482:Muscle, Skeletal / Q000378:metabolism*; D010710:Phosphates / Q000378:metabolism; D010725:Phosphocreatine / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar
D010710:Phosphates; D010725:Phosphocreatine; D000255:Adenosine Triphosphate; D006003:Glycogen; D003847:Deoxyglucose; D005947:Glucose