Translocations of 11q13 in mantle cell lymphoma fail to disrupt the S mu bp-2 gene.
We recently cloned a gene whose protein product binds to the Epstein-Barr virus BZLF1 gene promoter. The same gene has been previously cloned by another group who named it S mu bp-2 because its protein product binds to the S mu motif of the immunoglobulin heavy chain gene where it is postulated to function in immunoglobulin class switching. In the current study, we confirm that the S mu bp-2 gene is located on chromosome 11q13, a locus known to be altered by translocation in 50-70% of mantle cell lymphomas. We used Southern blot analysis to determine whether the S mu bp-2 gene was structurally rearranged in any of 25 mantle cell lymphomas. We found no evidence of rearrangement in any of these lymphomas including 18 that were proven to contain t(11;14) by cytogenetic analysis. These data suggest that structural alteration of the S mu bp-2 gene is not an underlying mechanism of tumorigenesis in mantle cell lymphomas.
Gulley
M L
ML
Department of Pathology, University of Texas Health Science Center at San Antonio (UTHSCSA) 78284-7750, USA. gulleym@uthscsa.edu
Zhang
Q
Q
Gascoyne
R D
RD
DuPont
B R
BR
Banks
P M
PM
Cho
C G
CG
Huang
J M
JM
Montalvo
E A
EA
D002874:Chromosome Mapping; D002880:Chromosomes, Human, Pair 11; D003001:Cloning, Molecular; D004268:DNA-Binding Proteins / Q000235:genetics*; D006367:HeLa Cells; D006801:Humans; D008228:Lymphoma, Non-Hodgkin / Q000235:genetics*; D008969:Molecular Sequence Data; D011401:Promoter Regions, Genetic; D015534:Trans-Activators / Q000235:genetics; D014157:Transcription Factors; D014178:Translocation, Genetic; D014764:Viral Proteins
C063676:BZLF1 protein, Herpesvirus 4, Human; D004268:DNA-Binding Proteins; C489892:IGHMBP2 protein, human; D015534:Trans-Activators; D014157:Transcription Factors; D014764:Viral Proteins