Human beta-migrating very low density lipoprotein induces foam cell formation in human mesangial cells.
To elucidate the mechanism of foam cell formation in the mesangial region of a kidney observed in a familial type III hyperlipoproteinemic patient presenting with diabetes mellitus and nephrotic syndrome, we have examined, in the present study, the effect of human beta-VLDL (apo E2/E2) on foam cell formation in human mesangial cells, since an increase in beta-VLDL is a characteristic feature of this patient. Human beta-VLDL (apo E2/E2) induced foam cell formation in human mesangial cells. The binding of [125I]LDL to human mesangial cells was inhibited completely by both LDL and beta-VLDL. On the other hand, the binding of [125I]beta-VLDL was completely inhibited by beta-VLDL, but partially by LDL. The LDL receptor, but not the VLDL receptor was down-regulated by accumulation of cholesteryl esters. These results suggest that human beta-VLDL (apo E2/E2)-induced foam cell formation in mesangial cells is mediated through both the LDL receptor pathway and the beta-VLDL specific pathway, in which the VLDL receptor is one of the candidates.
Anami
Y
Y
Department of Metabolic Medicine, Kumamoto University School of Medicine, Japan.
Kobori
S
S
Sakai
M
M
Kasho
M
M
Nishikawa
T
T
Yano
T
T
Matsuda
H
H
Matsumura
T
T
Takemura
T
T
Shichiri
M
M
D015152:Blotting, Northern; D002478:Cells, Cultured; D002788:Cholesterol Esters / Q000378:metabolism; D005487:Foam Cells / Q000187:drug effects / Q000378:metabolism / Q000473:pathology*; D005920:Glomerular Mesangium / Q000378:metabolism / Q000473:pathology*; D006801:Humans; D008024:Ligands; D008079:Lipoproteins, VLDL / Q000378:metabolism / Q000502:physiology*; D011485:Protein Binding / Q000187:drug effects
D002788:Cholesterol Esters; D008024:Ligands; D008079:Lipoproteins, VLDL