title: Rapid and reliable measurement of highly elevated blood ammonia concentrations in children.
abstract: Newborns and children may suffer from extremely high ammonia levels in their blood. We evaluated a fast, reliable micromethod, based on the Blood Ammonia Checker II (BAC II) in combination with the dilution with fresh whole blood. Comparison of the proposed method with an enzymatic method revealed a statistically significant correlation. We conclude that the dilution of patient's blood with fresh whole blood extends the measuring range of ammonia on the BAC II analyzer from 286 mumol/l to about 700 mumol/l.
authors.affiliation: Department of Clinical Chemistry, University Hospital St. Radboud, Nijmegen, The Netherlands.
authors.forename: M H
authors.initials: MH
authors.lastname: de Keijzer
authors.forename: B S
authors.initials: BS
authors.lastname: Jakobs
authors.forename: R W
authors.initials: RW
authors.lastname: Brandts
authors.forename: M T
authors.initials: MT
authors.lastname: Hofs
authors.forename: F J
authors.initials: FJ
authors.lastname: Trijbels
authors.forename: J A
authors.initials: JA
authors.lastname: Smeitink
mesh_terms: D000641:Ammonia / Q000097:blood*; D001774:Blood Chemical Analysis / Q000379:methods*; D002648:Child; D006801:Humans; D007231:Infant, Newborn; D008832:Microchemistry / Q000379:methods; D011933:Reagent Kits, Diagnostic; D015203:Reproducibility of Results
chemical_list: D011933:Reagent Kits, Diagnostic; D000641:Ammonia