The effect of anti-prolactin antibody on in vitro synthesis of a pregnancy-associated rat uterine glycoprotein.
Experiments were conducted to study the hormonal (estrogen [E2], progesterone [P4] and prolactin [PRL]), regulation of synthesis of a pregnancy-associated glycoprotein (named Uterine Agglutinin or UA) in the Day 4 post coital (p.c.) rat uterus with antibody administration and immunohistochemistry. Of the antibodies used, it was shown that anti-PRL antibody was the most effective in reducing in vitro UA synthesis. The results suggested that in vitro UA synthesis could be correlated to serum PRL levels as analyzed by radio-immunoassay. Binding studies revealed that PRL bound specifically to the stromal cells of the rat uterus where UA is produced and localized.
Mehta
M
M
Bose Institute, Department of Biochemistry, Calcutta, India.
Chowdhury
M
M
D000373:Agglutinins / Q000008:administration & dosage / Q000096:biosynthesis* / Q000276:immunology; D000818:Animals; D000906:Antibodies / Q000008:administration & dosage / Q000494:pharmacology*; D000918:Antibody Specificity; D005260:Female; D006023:Glycoproteins / Q000008:administration & dosage / Q000096:biosynthesis* / Q000276:immunology; D007122:Immunoelectrophoresis; D007150:Immunohistochemistry; D007274:Injections, Intraperitoneal; D009928:Organ Specificity; D011247:Pregnancy; D011257:Pregnancy Proteins / Q000008:administration & dosage / Q000096:biosynthesis / Q000276:immunology; D011388:Prolactin / Q000097:blood / Q000276:immunology* / Q000378:metabolism*; D011485:Protein Binding; D051381:Rats; D017207:Rats, Sprague-Dawley; D014599:Uterus / Q000166:cytology / Q000378:metabolism
D000373:Agglutinins; D000906:Antibodies; D006023:Glycoproteins; D011257:Pregnancy Proteins; C057612:sialic acid specific agglutinin protein, rat; D011388:Prolactin