Interaction of alpha-actinin with cellular titin.
Cellular titin (c-titin) colocalizes with myosin II in cytoskeletal structures containing actin in vivo and organizes highly ordered myosin bipolar filament arrays in the absence of actin in vitro. We report here that the actin-binding protein alpha-actinin associates with coassemblies of c-titin and myosin through direct interaction with c-titin. These results support the possibility that interaction between the myosin-associated protein c-titin and the actin-associated protein alpha-actinin organizes and stabilizes actin-myosin II cytoskeletal structures in vivo.
Eilertsen
K J
KJ
Molecular Biophysics Program, Florida State University, Tallahassee 32306-3050, USA.
Kazmierski
S T
ST
Keller
T C
TC
D000185:Actinin / Q000378:metabolism*; D000818:Animals; D002499:Centrifugation, Density Gradient; D002645:Chickens; D064211:Connectin; D009124:Muscle Proteins / Q000378:metabolism*; D009218:Myosins / Q000378:metabolism; D011494:Protein Kinases / Q000378:metabolism*; D013395:Sucrose
D064211:Connectin; D009124:Muscle Proteins; D000185:Actinin; D013395:Sucrose; D011494:Protein Kinases; D009218:Myosins