title: The HLA-A*0207 peptide binding repertoire is limited to a subset of the A*0201 repertoire.
abstract: Quantitative A*0207 peptide binding assays have been developed utilizing HLA transfected cells and affinity purified molecules. By using a panel of single substitution analog peptides, it was demonstrated that A*0207 binds peptides with main anchor specificity at position 2 and the C-terminus similar to A*0201. Previous data indicating that A*0207 (but not A*0201) also requires the presence of D or P in position 3 of its peptide ligands was confirmed by the analysis of additional single substituted analogs. Finally, by analyzing the A*0201 and A*0207 binding capacities of panels of unrelated synthetic peptides, it was found that 8/15 (53.3%) A*0201 binders with D or P in position 3 bound A*0207, while only 5/72 (6.9%) A*0201 binders without D or P in position 3 also bound A*0207. Together, these data indicate that although A*0207 may be included amongst A2 supertype alleles, its peptide binding repertoire is largely limited to a subset of that bound by A*0201.
authors.affiliation: Cytel Corporation, San Diego, CA 92121, USA.
authors.forename: J
authors.initials: J
authors.lastname: Sidney
authors.forename: M F
authors.initials: MF
authors.lastname: del Guercio
authors.forename: S
authors.initials: S
authors.lastname: Southwood
authors.forename: G
authors.initials: G
authors.lastname: Hermanson
authors.forename: A
authors.initials: A
authors.lastname: Maewal
authors.forename: E
authors.initials: E
authors.lastname: Appella
authors.forename: A
authors.initials: A
authors.lastname: Sette
mesh_terms: D017951:Antigen Presentation / Q000276:immunology; D001665:Binding Sites; D002461:Cell Line, Transformed; D015789:HLA-A2 Antigen / Q000235:genetics / Q000276:immunology*; D006801:Humans; D010455:Peptides / Q000276:immunology*
chemical_list: D015789:HLA-A2 Antigen; D010455:Peptides