Glycohemoglobin filter assay for doctors' offices based on boronic acid affinity principle.
We present a new filter assay for the determination of glycohemoglobin as a unique application of the boronic acid affinity principle. With the use of a water-soluble blue-colored boronic acid derivative and a specific precipitation method for hemoglobin, total hemoglobin including bound boronic acid is precipitated and collected on a filter strip before quantification. Hemoglobin and boronic acid are quantified by a dual-wave-length reflectometric measurement, and the result is reported directly as percent glycohemoglobin. The test is simple, quick, and designed as a doctors' office test for the monitoring and management of diabetes. The imprecision of the assay is < 4% over the range 3-18% Hb A1c, and the method is linear up to at least 20% Hb A1c. Comparisons with four well-established glycohemoglobin methods yielded correlation coefficients ranging from 0.94 to 0.99, with slopes from 0.94 to 1.01.
Frantzen
F
F
Axis Nord, Mørkved, Norway. frank.frantzin@axisbio.no
Grimsrud
K
K
Heggli
D E
DE
Faaren
A L
AL
Løvli
T
T
Sundrehagen
E
E
D000818:Animals; D001897:Boronic Acids; D002417:Cattle; D004396:Coloring Agents; D005374:Filtration / Q000295:instrumentation; D006442:Glycated Hemoglobin / Q000032:analysis*; D006801:Humans; D008875:Middle Aged; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013053:Spectrophotometry
D001897:Boronic Acids; D004396:Coloring Agents; D006442:Glycated Hemoglobin