Distribution of mouse adenovirus type 1 in intraperitoneally and intranasally infected adult outbred mice.
In situ nucleic acid hybridization and immunohistochemistry were used to determine the histological localization of mouse adenovirus type 1 (MAV-1) during acute infection of adult mice infected either intraperitoneally or intranasally with 1,000 PFU of wild-type virus. Organ samples were collected from days 1 to 17 postinfection for the intraperitoneally infected mice and from days 1 to 13 for the intranasally infected mice. Endothelial cells of the brain and spinal cord showed extensive evidence of MAV-1 infection. Endothelial cells in lungs, kidneys, and other organs were also positive for MAV-1, indicating a widespread involvement of the systemic circulation. The presence of viral nucleic acid and/or antigen was also demonstrated in lymphoid tissue. The spleens, Peyer's patches, and peripheral lymph nodes showed positive staining at various times postinfection in mice infected by either route. Virus-infected cells in the spleen exhibited a stellate shape and were localized to the red pulp and germinal centers, suggesting that they are cells of the mononuclear phagocytic system.
Kajon
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Department of Genetics, Franklin College of Arts and Sciences, University of Georgia, Athens 30602-7223, USA.
Brown
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CC
Spindler
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KR
D000257:Adenoviridae Infections / Q000821:virology*; D000818:Animals; D007150:Immunohistochemistry; D008198:Lymph Nodes / Q000821:virology; D017921:Mastadenovirus; D051379:Mice; D009296:Nasal Cavity / Q000821:virology; D009928:Organ Specificity; D010529:Peritoneal Cavity / Q000821:virology