Measurement of cytotoxicity of sulphonated chloroaluminium phthalocyanine on various cell systems.
Phthalocyanines (ClAlPcS) present a new generation of substances for photodynamic treatment of tumors. To find optimal therapeutic doses for i.v. or local application, it is necessary to test the maximal non-toxic concentration on model systems. As a standard testing system (cellular substrate) for definition of the in vitro cytotoxicity, human lymphocytes separated from peripheral human blood and splenocytes obtained from rabbit spleen fragments were chosen. The vitality test on human peripheral lymphocytes proved that the used concentrations in the range 0.1-1.4 mg/ml did not significantly influence vitality of the cellular substrate. The test used for determination of the migration activity did not show any influence on MI in the concentration range 0.1-0.5 mg/ml. The higher concentrations tested (range 0.6-1.4 mg/ml) lead to reduction of the migration activity presented by a decrease in the migration index MI: for c = 0.6 mg/ml, MI = 0.85; for c = 1.4 mg/ml, MI = 0.45 (normal value for MI is in the range 0.9-1.1).
Kolárová
H
H
Department of Biophysics, Medical Faculty, Palacký University, Olomouc, Czech Republic.
Ditrichová
D
D
Rakusan
J
J
Jírová
D
D
D000818:Animals; D000903:Antibiotics, Antineoplastic / Q000009:adverse effects*; D003602:Cytotoxicity, Immunologic; D006801:Humans; D066298:In Vitro Techniques; D007211:Indoles / Q000633:toxicity*; D008214:Lymphocytes / Q000187:drug effects*; D009942:Organometallic Compounds / Q000633:toxicity*; D011817:Rabbits; D011838:Radiation-Sensitizing Agents / Q000633:toxicity*
D000903:Antibiotics, Antineoplastic; D007211:Indoles; D009942:Organometallic Compounds; D011838:Radiation-Sensitizing Agents; C064331:aluminum phthalocyanine disulfonate