Specific interactions between human integrin alpha v beta 3 and chimeric hepatitis B virus core particles bearing the receptor-binding epitope of foot-and-mouth disease virus.
Purified integrin alpha v beta 3 was used in solid-phase binding studies with chimeric hepatitis B cores which carry the RGD-containing loop of VP1 protein of the foot-and-mouth disease virus (FMDV). High levels of specific binding between the integrin and the particles were detected by enzyme-linked immunosorbent assays. The binding was Mn2+ cation dependent and could be competed with fibronectin, vitronectin, and the peptide GRGDSPK. Particles in which the RGD motif had been mutated to RGE failed to bind, indicating that the chimeric cores bound specifically to the ligand binding site of integrin alpha v beta 3. Electron micrographs showed several individual alpha v beta 3 molecules bound to the surface of each chimeric particle. Collectively, these data constitute firm evidence that the RGD-containing loop of FMDV is critical for binding to alpha v beta 3 and provide support for identification of alpha v beta 3 as a potential cellular receptor for FMDV.
Sharma
A
A
Laboratory of Molecular Biophysics, Oxford, United Kingdom.
Rao
Z
Z
Fry
E
E
Booth
T
T
Jones
E Y
EY
Rowlands
D J
DJ
Simmons
D L
DL
Stuart
D I
DI
D000956:Antigens, Viral / Q000235:genetics / Q000378:metabolism*; D005537:Aphthovirus / Q000235:genetics / Q000378:metabolism*; D001665:Binding Sites; D006515:Hepatitis B virus / Q000235:genetics / Q000378:metabolism*; D006801:Humans; D008854:Microscopy, Electron; D011485:Protein Binding; D011956:Receptors, Cell Surface / Q000235:genetics / Q000378:metabolism; D019030:Receptors, Vitronectin / Q000378:metabolism* / Q000648:ultrastructure; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000378:metabolism* / Q000648:ultrastructure; D014758:Viral Core Proteins / Q000235:genetics / Q000378:metabolism*
D000956:Antigens, Viral; D011956:Receptors, Cell Surface; D019030:Receptors, Vitronectin; D011993:Recombinant Fusion Proteins; D014758:Viral Core Proteins