Molecular cloning and gene expression of growth hormone-releasing peptide receptor in rat tissues.
We cloned a fragment of the rat GH-releasing peptide (GHRP) receptor homologue and examined the tissue distribution of GHRP receptor mRNA in rats. Sequence analysis showed that the open reading frame is well conserved between rat and human with 96% identity in a 364-amino acid overlap. By reverse transcription-polymerase chain reaction we detected GHRP receptor mRNAs in the rat brain including the hypothalamus, anterior pituitary, and renal pelvis in twenty-eight tissues tested. Microdissection revealed that GHRP receptor mRNAs were localized predominantly in the arcuate nucleus and ventromedial hypothalamus.
Yokote
R
R
First Department of Internal Medicine, Kagawa Medical University, Japan.
Sato
M
M
Matsubara
S
S
Ohye
H
H
Niimi
M
M
Murao
K
K
Takahara
J
J
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D001923:Brain Chemistry; D003001:Cloning, Molecular; D017124:Conserved Sequence / Q000235:genetics; D015870:Gene Expression / Q000235:genetics; D006801:Humans; D007031:Hypothalamus / Q000737:chemistry; D008969:Molecular Sequence Data; D012333:RNA, Messenger / Q000032:analysis / Q000235:genetics; D051381:Rats; D017208:Rats, Wistar; D018013:Receptors, Neuropeptide / Q000032:analysis / Q000235:genetics*; D018017:Receptors, Pituitary Hormone-Regulating Hormone / Q000032:analysis / Q000235:genetics*; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D014018:Tissue Distribution
D012333:RNA, Messenger; D018013:Receptors, Neuropeptide; D018017:Receptors, Pituitary Hormone-Regulating Hormone; C048250:somatotropin releasing hormone receptor