Different RAPD patterns between Metagonimus yokogawai and Metagonimus Miyata type.
Genomic DNA from Metagonimus yokogawai and Metagonimus Miyata type was amplified by polymerase chain reaction based on the random amplification of polymorphic DNA (RAPD) technique. Eight random 10-mer oligonucleotide primers (OPA-02, 5-TGCCGAGCTG-3; OPA-09, 5-GGGTAACGCC-3; OPA-10, 5-GTGATCGCAG-3; OPA-11, 5-CAATCGCCGT-3; OPA-13, 5-CAGCACCCAC-3; OPA-17, 5-GACCGCTTGT-3; OPA-19, 5-CAAACGTCGG-3; OPA-20, 5-GTTGCGATCC-3) with a G + C content of 60-70% (Kit A, Operon Technologies Inc., California, USA) could produce distinguishable banding patterns between the two Metagonimus species. From the results of this study, it was suggested that Metagonimus Miyata type has a different DNA sequence from M. Yokogawai.
Yu
J R
JR
Department of Parasitology, College of Medicine, Kon-Kuk University, Chungju, Korea. jaeran.yu@kcucc.konkuk.ac.kr
Chung
J S
JS
Chai
J Y
JY
D000818:Animals; D001483:Base Sequence; D017931:DNA Primers; D018509:DNA, Helminth / Q000032:analysis* / Q000235:genetics; D016678:Genome; D006578:Heterophyidae / Q000145:classification / Q000235:genetics*; D016133:Polymerase Chain Reaction / Q000379:methods
D017931:DNA Primers; D018509:DNA, Helminth