Solid-phase extraction with end-capped C2 columns for the routine measurement of racemic citalopram and metabolites in plasma by high-performance liquid chromatography.
An assay based on solid-phase extraction followed by high-performance liquid chromatography (HPLC) was developed for the measurement of citalopram and its main metabolites desmethylcitalopram and didesmethylcitalopram. The best extraction procedure was performed with end-capped C2 column utilising secondary silanol interactions to obtain clean extract. The HPLC analysis was done on a phenyl column with a mobile phase without any amine additives. Fluorescence detection gave a limit of detection of 0.8 nmol/l plasma for the compounds analysed.
Carlsson
B
B
Department of Clinical Pharmacology, Faculty of Health Sciences, Linköping University, Sweden.
Norlander
B
B
D000928:Antidepressive Agents / Q000097:blood* / Q000737:chemistry / Q000378:metabolism; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D002940:Circadian Rhythm; D015283:Citalopram / Q000097:blood* / Q000737:chemistry / Q000378:metabolism; D004347:Drug Interactions; D004350:Drug Residues / Q000032:analysis*; D006801:Humans; D016014:Linear Models; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
D000928:Antidepressive Agents; D015283:Citalopram