title: An outbreak of epidemic keratoconjunctivitis caused by adenovirus type 37.
abstract: An outbreak of acute keratoconjunctivitis in an ophthalmology department affected 15 patients and seven members of staff and necessitated temporary closure of the unit. Adenovirus (Ad) was isolated from eye swabs taken during the outbreak, but typing of the isolates by virus neutralisation assay proved unsatisfactory, wrongly assigning the isolate to serotype 10. A robust and rapid method for preparing microgram amounts of Ad DNA from infected cells was devised and used as the basis of a non-radioactive method for routine genotyping of adenovirus clinical isolates. All isolates associated with the outbreak, and one from a patient presenting at a nearby hospital during the outbreak, were found to have restriction endonuclease digestion patterns characteristic of Ad 37.
authors.affiliation: Department of Medical Microbiology, University Hospital of Wales, Heath Park, Cardiff.
authors.forename: S
authors.initials: S
authors.lastname: Curtis
authors.forename: G W
authors.initials: GW
authors.lastname: Wilkinson
authors.forename: D
authors.initials: D
authors.lastname: Westmoreland
mesh_terms: D000208:Acute Disease; D000258:Adenovirus Infections, Human / Q000453:epidemiology / Q000821:virology*; D000260:Adenoviruses, Human / Q000145:classification* / Q000235:genetics; D003428:Cross Infection / Q000453:epidemiology / Q000821:virology*; D004279:DNA, Viral / Q000032:analysis; D004196:Disease Outbreaks; D004587:Electrophoresis, Agar Gel; D005838:Genotype; D006757:Hospital Units; D006801:Humans; D007637:Keratoconjunctivitis / Q000453:epidemiology / Q000821:virology*; D009500:Neutralization Tests; D009784:Occupational Diseases / Q000453:epidemiology / Q000821:virology; D009885:Ophthalmology; D010564:Personnel, Hospital; D015183:Restriction Mapping; D012703:Serotyping; D014852:Wales / Q000453:epidemiology
chemical_list: D004279:DNA, Viral