Regulatory region of expression of Thiobacillus ferrooxidans leuB gene in Escherichia coli.
The regulatory region of Thiobacillus ferrooxidans leuB gene was analyzed. The deletion analysis indicated that the promoter sequences CATCCG and TATTAT, which were similar to the consensus -35 and -10 sequences of Escherichia coli, respectively, existed. The transcriptional initiation site was located at the position of cytosine -26. The deletion analysis of the upstream region suggested the existence of a regulatory region by leucine and the region related to transcription of the gene.
Kawaguchi
H
H
Department of Bioresources Chemistry, Faculty of Agriculture, Okayama University, Japan.
Inagaki
K
K
Tanaka
H
H
D050539:3-Isopropylmalate Dehydrogenase; D000429:Alcohol Oxidoreductases / Q000235:genetics*; D001483:Base Sequence; D004926:Escherichia coli / Q000235:genetics*; D015964:Gene Expression Regulation, Bacterial / Q000235:genetics*; D005798:Genes, Bacterial; D007930:Leucine / Q000096:biosynthesis; D008969:Molecular Sequence Data; D010957:Plasmids; D011401:Promoter Regions, Genetic / Q000235:genetics; D013855:Thiobacillus / Q000235:genetics*
D000429:Alcohol Oxidoreductases; D050539:3-Isopropylmalate Dehydrogenase; D007930:Leucine