Carbon tetrachloride does not induce micronucleus in either mouse bone marrow or peripheral blood.
We performed mouse bone marrow and peripheral blood micronucleus tests on carbon tetrachloride (CCl4). In the bone marrow assay, bone marrow cells were collected once after 24 h and twice, with a 24-h interval at a dose of 500, 1000 and 2000 mg/kg. In the peripheral blood assay, blood samples were collected 0, 24, 48 and 72 h after a single intraperitoneal injection at a dose of 1000, 2000 and 3000 mg/kg. As a result, micronucleated polychromatic erythrocytes (MNPCEs) were observed neither in the bone marrow assay nor the peripheral blood assay. We concluded that CCl4 does not induce chromosomal aberrations in the mouse bone marrow cells under these experimental conditions.
Suzuki
H
H
Research Center, Taisho Pharmaceutical Co., Ltd., Saitama, Japan.
Hirano
N
N
Watanabe
C
C
Tarumoto
Y
Y
D000818:Animals; D001769:Blood; D001853:Bone Marrow / Q000187:drug effects* / Q000648:ultrastructure; D002251:Carbon Tetrachloride / Q000633:toxicity*; D008297:Male; D051379:Mice; D015162:Micronucleus Tests; D009152:Mutagenicity Tests
D002251:Carbon Tetrachloride