Protein A carrying PMMA microbeads: adsorption of cholesterol and HlgG from human plasma.
Cholesterol and HIgG adsorbed from human plasma obtained from a hypercholesterolemic patient, onto protein A-immobilized polymethyl-methacrylate uniform microbeads carrying different amounts of protein A (0.264-1.682 mg protein A/g PMMA, or 0.66-4.2 mg protein A/m2 PMMA) were investigated in batchwise experiments. There was no interaction between protein A molecules and cholesterol when cholesterol aqueous solutions were used. However, there was significant cholesterol and HIgG adsorption from the plasma obtained from a patient with hypercholesterolemia. The maximum amounts of cholesterol and HIgG adsorbed were 3.96 micromol cholesterol/g PMMA (5.4 mg cholesterol/g PMMA) and 0.242 micromol IgG/g PMMA (35.4 mg IgG/g PMMA).
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Chemical Engineering Department, Hacettepe University, Beytepe, Ankara, Turkey.
Ayhan
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H
Pişkin
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D002784:Cholesterol / Q000302:isolation & purification*; D002846:Chromatography, Affinity; D006801:Humans; D006937:Hypercholesterolemia / Q000097:blood*; D008863:Microspheres; D009842:Oligopeptides / Q000302:isolation & purification*; D019904:Polymethyl Methacrylate; D013205:Staphylococcal Protein A
D009842:Oligopeptides; D013205:Staphylococcal Protein A; C029275:hippuryl-glycyl-glycine; D019904:Polymethyl Methacrylate; D002784:Cholesterol