title: A lectin from the bark of the rubber tree (Hevea brasiliensis).
abstract: A protein isolated and purified from extracts of bark strips from mature rubber (Hevea brasilinsis) agglutinated erythrocytes from rabbits and all human blood types except AB. No agglutination was detected with erythrocytes from sheep, rates or mice. Proteolytic treatment of Hevea bark lectin (HBL) abolished haemagglutinin activity. The M(r) determined by SDS-PAGE was 40 k and that estimated from gel filtration was 140 k. Fetuin, asialofetuin, bovine submaxillary mucin and asialosubmaxillary mucin inhibited HBL-induced agglutination of rabbit erythrocytes. The HBL showed maximum haemagglutination activity over the pH range 4.5-9.5 and heat stability up to 60 degrees.
authors.affiliation: Department of Biochemistry, Faculty of Science, Prince of Songkia University, Hat-Yai, Thailand.
authors.forename: R
authors.initials: R
authors.lastname: Wititsuwannakul
authors.forename: D
authors.initials: D
authors.lastname: Wititsuwannakul
authors.forename: C
authors.initials: C
authors.lastname: Sakulborirug
mesh_terms: D000017:ABO Blood-Group System; D000818:Animals; D002241:Carbohydrates; D002417:Cattle; D002848:Chromatography, DEAE-Cellulose; D004912:Erythrocytes / Q000187:drug effects; D019665:Euphorbiaceae / Q000276:immunology; D006386:Hemagglutination Tests; D006801:Humans; D037102:Lectins / Q000302:isolation & purification* / Q000494:pharmacology; D051379:Mice; D037121:Plant Lectins; D018547:Plant Stems; D011485:Protein Binding; D011817:Rabbits; D051381:Rats; D012408:Rubber; D012756:Sheep
chemical_list: D000017:ABO Blood-Group System; D002241:Carbohydrates; D037102:Lectins; D037121:Plant Lectins; D012408:Rubber