title: Heat-shock of normal t-cells and T-cell lines downregulates the TCR/CD3-mediated cytoplasmic Ca2+ responses and the production of inositol trisphosphate.
abstract: The application of heat shock to different types of cells is known to cause multiple biochemical and metabolic changes. The predominant event though is an increased synthesis and expression of a family of proteins, the heat-shock proteins (Hsp). Some of these proteins are currently considered to be involved in the signal transduction pathway. We investigated for any possible effects of heating fresh normal peripheral T-cells and T-cell lines, on the early signal transduction events which follow the antigen (Ag) receptor-complex (TCR/CD3) crosslinking. More specifically, the Ag-receptor-initiated free cytoplasmic Ca2+ ([Ca2+]i) responses and the production of inositol 1,4,5-trisphosphate (IP3) were evaluated. Heating fresh unmanipulated peripheral T-cells 8 hours before the TCR/CD3 stimulation resulted in decreased [Ca2+]i responses. This was also true for cells of normal short-term T-cell lines as well. The TCR/CD3-mediated production of IP3, which is a mediator of the [Ca2+]i response, was also decreased in heat-shocked T-cells.
authors.affiliation: Department of Clinical Physiology, Walter Reed Army Institute of Research, Washington, DC 20307-5100, USA.
authors.forename: S N
authors.initials: SN
authors.lastname: Liossis
authors.forename: G C
authors.initials: GC
authors.lastname: Tsokos
mesh_terms: D000818:Animals; D017252:CD3 Complex / Q000502:physiology*; D002118:Calcium / Q000378:metabolism*; D002460:Cell Line; D003593:Cytoplasm / Q000378:metabolism; D015536:Down-Regulation / Q000502:physiology; D018840:HSP70 Heat-Shock Proteins / Q000096:biosynthesis; D018869:Heat-Shock Response / Q000502:physiology*; D006801:Humans; D015544:Inositol 1,4,5-Trisphosphate / Q000096:biosynthesis*; D051379:Mice; D011948:Receptors, Antigen, T-Cell / Q000502:physiology*; D013601:T-Lymphocytes / Q000378:metabolism* / Q000502:physiology / Q000648:ultrastructure
chemical_list: D017252:CD3 Complex; D018840:HSP70 Heat-Shock Proteins; D011948:Receptors, Antigen, T-Cell; D015544:Inositol 1,4,5-Trisphosphate; D002118:Calcium