title: Arrestin and phosducin are expressed in a small number of brain cells.
abstract: Retinal photoreceptor rods and pinealocytes contain well-characterized proteins such as arrestin and phosducin whose expression is highly restricted to these cell types. Transgenic mice having a LacZ gene under the control of an arrestin promoter expressed beta-galactosidase (beta-Gal) in the photoreceptor rods and pinealocytes. In addition, it was expressed in very small numbers of discrete cells in the habenular commissura, amygdala, ventral tegmental area and superior colliculus of the brain. Immunocytochemical studies with antibody probes revealed that high level of arrestin and phosducin were also found in the same cell types. Furthermore melatonin was found in those cells of the habenula commissura. The results indicate that novel cell types are present in the brain tissues. Since high levels of arrestin and phosducin expression are generally restricted to photoreceptor rod cells and pinealocytes, these data suggest that certain brain cells may have functions similar to pinealocytes.
authors.affiliation: Brigham and Women's Hospital and Department of Ophthalmology, Harvard Medical School, Boston, MA 02115, USA.
authors.forename: K
authors.initials: K
authors.lastname: Sunayashiki-Kusuzaki
authors.forename: T
authors.initials: T
authors.lastname: Kikuchi
authors.forename: E F
authors.initials: EF
authors.lastname: Wawrousek
authors.forename: T
authors.initials: T
authors.lastname: Shinohara
mesh_terms: D000818:Animals; D019393:Arrestin / Q000235:genetics*; D001921:Brain / Q000166:cytology / Q000378:metabolism*; D005136:Eye Proteins / Q000235:genetics*; D020555:GTP-Binding Protein Regulators; D015870:Gene Expression; D017930:Genes, Reporter; D051379:Mice; D008822:Mice, Transgenic; D009419:Nerve Tissue Proteins / Q000235:genetics*; D010750:Phosphoproteins / Q000235:genetics*; D011401:Promoter Regions, Genetic; D001616:beta-Galactosidase / Q000235:genetics*
chemical_list: D019393:Arrestin; D005136:Eye Proteins; D020555:GTP-Binding Protein Regulators; D009419:Nerve Tissue Proteins; D010750:Phosphoproteins; C065631:phosducin; D001616:beta-Galactosidase