p53-dependent response in UV-induced transformation.
Cells carrying different numbers of copies of wild-type p53 were transformed by UV irradiation. Putative dense foci were analyzed for their growth behavior in low serum and for anchorage-independence. A correlation was found between number of copies of wild-type p53 gene and transformation frequency: cells with more copies of p53 gene were more resistant to transformation by UV. The p53 in the transformed clones was shown to be wild-type at the protein level. A single DNA base change was detected between GenBank data and our normal and transformed cells; this pre-existing single base difference is probably due to the differences between the strains of mice from which the cells were derived.
Zhang
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Department of Chemical Biology, College of Pharmacy, Rutgers University, Piscataway, NJ 08855, USA.
Zhang
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Zhu
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Chen
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D000818:Animals; D001483:Base Sequence; D002471:Cell Transformation, Neoplastic / Q000528:radiation effects*; D002478:Cells, Cultured; D016158:Genes, p53; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D011233:Precipitin Tests; D016159:Tumor Suppressor Protein p53 / Q000502:physiology*; D014466:Ultraviolet Rays
D016159:Tumor Suppressor Protein p53