title: Infrequent genetic alterations of the PTEN/MMAC1 gene in Japanese patients with primary cancers of the breast, lung, pancreas, kidney, and ovary.
abstract: In the present study, we searched for genetic alterations of the entire coding region of PTEN/MMAC1, a recently isolated candidate tumor suppressor gene, in 178 specimens from Japanese patients with various malignant tumors by the polymerase chain reaction-single strand conformation polymorphism method. The samples consisted of 11 glioblastoma multiformes (GBMs), 14 astrocytomas, 47 breast cancers, 25 non-small cell lung cancers, 9 small cell lung cancers, 8 pancreatic cancers, 24 renal cell carcinomas, 20 ovarian cancers, and 20 metastatic lung tumors from various organs. Only one somatic frameshift mutation at codon 319 was observed in one (9%) of eleven GBMs. Our results suggest that mutation of the PTEN/MMAC1 gene does not play a major role in carcinogenesis, at least in the tumor types from Japanese patients analyzed in this study.
authors.affiliation: Department of Molecular Pathology, Tohoku University School of Medicine, Sendai.
authors.forename: A
authors.initials: A
authors.lastname: Sakurada
authors.forename: A
authors.initials: A
authors.lastname: Suzuki
authors.forename: M
authors.initials: M
authors.lastname: Sato
authors.forename: H
authors.initials: H
authors.lastname: Yamakawa
authors.forename: K
authors.initials: K
authors.lastname: Orikasa
authors.forename: S
authors.initials: S
authors.lastname: Uyeno
authors.forename: T
authors.initials: T
authors.lastname: Ono
authors.forename: N
authors.initials: N
authors.lastname: Ohuchi
authors.forename: S
authors.initials: S
authors.lastname: Fujimura
authors.forename: A
authors.initials: A
authors.lastname: Horii
mesh_terms: D001483:Base Sequence; D001932:Brain Neoplasms / Q000235:genetics; D001943:Breast Neoplasms / Q000235:genetics; D004273:DNA, Neoplasm / Q000235:genetics; D005260:Female; D016147:Genes, Tumor Suppressor; D005909:Glioblastoma / Q000235:genetics; D006801:Humans; D007680:Kidney Neoplasms / Q000235:genetics; D019656:Loss of Heterozygosity; D008175:Lung Neoplasms / Q000235:genetics; D009154:Mutation; D009369:Neoplasms / Q000235:genetics*; D010051:Ovarian Neoplasms / Q000235:genetics; D010190:Pancreatic Neoplasms / Q000235:genetics; D018807:Polymorphism, Single-Stranded Conformational
chemical_list: D004273:DNA, Neoplasm