title: Immunological and electronmicroscopic studies in pigs infected with Yersinia enterocolitica 0:3.
abstract: The cellular immune response after an experimental oral infection with Yersinia enterocolitica (serotype 0:3, biotype 4, harbouring the virulence plasmid-p YV) was studied in pigs. A maximal stimulation of the T cell population in the thymus, spleen and peripheral blood was stated on the 15th day post infection (p.i.) by the rosette forming cell (RFC) test. The hemolysins (produced by B cells and detected by the plaque forming cell test-PFC) were significantly increased on the 15th day p.i. among the thymus and spleen lymphocytes and on the 25th day p.i. among the blood lymphocytes. Blood and thymus lymphocytes were activated faster by the infectious agent in comparison to the spleen cells. The electronmicroscopic studies revealed an intracellular presence of the bacteria in alveolar macrophages (aMa) and peritoneal macrophages (pMa) as well as in Peyer's patches and tonsils as early as on the 4th day p.i. Extracellularly located bacteria were observed, too. The results have shown that inspite of the activation of T and B cell immune response, this infectious agent persisted in the porcine organism.
authors.affiliation: Institute of Microbiology, Bulgarian Academy of Sciences, Sofia, Bulgaria.
authors.forename: S
authors.initials: S
authors.lastname: Nikolova
authors.forename: H
authors.initials: H
authors.lastname: Najdenski
authors.forename: D
authors.initials: D
authors.lastname: Wesselinova
authors.forename: A
authors.initials: A
authors.lastname: Vesselinova
authors.forename: D
authors.initials: D
authors.lastname: Kazatchca
authors.forename: P
authors.initials: P
authors.lastname: Neikov
mesh_terms: D000818:Animals; D001402:B-Lymphocytes / Q000276:immunology; D013154:Spleen / Q000166:cytology; D013552:Swine; D013553:Swine Diseases / Q000276:immunology* / Q000473:pathology; D013601:T-Lymphocytes / Q000166:cytology / Q000276:immunology; D013950:Thymus Gland / Q000166:cytology; D015009:Yersinia Infections / Q000276:immunology* / Q000473:pathology / Q000662:veterinary; D015008:Yersinia enterocolitica / Q000276:immunology*