title: Detection of Theileria sergenti schizonts in bovine lymph node.
abstract: The growth of Theileria sergenti schizonts in bovine lymph nodes was examined by experimental infection. Six naive Holstein calves were inoculated with a sporozoite stabilate into the superficial cervical and subiliac lymph nodes. Biopsy samples from the inoculated calves were collected every 24 h for 10 days. Histological examination demonstrated the schizont of T. sergenti on day 4 post-inoculation. The growth of schizonts in the lymph node was observed on days 4-8 post-inoculation. Electron microscopic observation disclosed that the core of the schizont has a high density central core surrounded by numerous electron-dense spherules.
authors.affiliation: School of Veterinary Medicine, Rakuno Gakuen University, Hokkaido, Japan.
authors.forename: K
authors.initials: K
authors.lastname: Hagiwara
authors.forename: K
authors.initials: K
authors.lastname: Takahashi
authors.forename: H
authors.initials: H
authors.lastname: Taniyama
authors.forename: S
authors.initials: S
authors.lastname: Kawamoto
authors.forename: T
authors.initials: T
authors.lastname: Kurosawa
authors.forename: K
authors.initials: K
authors.lastname: Ikuta
authors.forename: C
authors.initials: C
authors.lastname: Ishihara
mesh_terms: D000818:Animals; D000953:Antigens, Protozoan / Q000302:isolation & purification; D001706:Biopsy; D002417:Cattle; D007150:Immunohistochemistry; D008198:Lymph Nodes / Q000469:parasitology*; D016794:Theileria / Q000254:growth & development* / Q000648:ultrastructure; D013801:Theileriasis / Q000469:parasitology*
chemical_list: D000953:Antigens, Protozoan