title: Immunologic protection against canine heartworm infection.
abstract: This study was conducted to evaluate protective efficiency of three different protocols for vaccination in canine heartworm infection. To evaluate the three protocols of immunization, dogs were separately immunized with living larvae; 1) immunization with gamma-attenuated infective larvae, 2) with 50 micrograms/kg ivermectin-abbreviation, and 3) with chemical abbreviation plus Freund's complete adjuvant (FCA). Each group was composed of two dogs. All dogs used for this study were subcutaneously challenged with 100 intact third-stage larvae (L3) various days after the last immunization, and the worms in the pulmonary arteries and the right ventricle of the heart were recovered 17 to 25 weeks post-infection. The numbers and the sexes of the worms were determined. A mean of 38 worms was burdened in the group immunized with irradiated L3, 36 worms in the chemically-abbreviated group, but 15.5 worms in the group with chemical abbreviation plus FCA. The percentages of the protection in the former two groups were nearly 50%, but 72.3% in the group with ivermectin plus FCA. The adjuvant enhanced the protective immunity against L3 challenge. Obvious eosinophilia was observed in both immunized and control dogs except for two dogs. There was no correlation between the suppression of eosinophilia and the protective immunity in the present study.
authors.affiliation: Animal Science Division, Dainippon Pharmaceutical Co., Ltd., Osaka, Japan.
authors.forename: M
authors.initials: M
authors.lastname: Yoshida
authors.forename: K
authors.initials: K
authors.lastname: Nakagaki
authors.forename: S
authors.initials: S
authors.lastname: Nogami
authors.forename: R
authors.initials: R
authors.lastname: Harasawa
authors.forename: R
authors.initials: R
authors.lastname: Maeda
authors.forename: H
authors.initials: H
authors.lastname: Katae
authors.forename: Y
authors.initials: Y
authors.lastname: Hayashi
mesh_terms: D000818:Animals; D000909:Antibodies, Helminth / Q000276:immunology*; D004183:Dirofilaria immitis / Q000276:immunology* / Q000528:radiation effects; D004184:Dirofilariasis / Q000097:blood / Q000276:immunology / Q000517:prevention & control*; D004283:Dog Diseases / Q000097:blood / Q000276:immunology / Q000517:prevention & control*; D004285:Dogs; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods / Q000662:veterinary; D004804:Eosinophils / Q000473:pathology; D005260:Female; D005620:Freund's Adjuvant / Q000008:administration & dosage* / Q000276:immunology; D007114:Immunization / Q000379:methods / Q000662:veterinary*; D007814:Larva / Q000276:immunology / Q000528:radiation effects; D007958:Leukocyte Count / Q000662:veterinary; D008297:Male
chemical_list: D000909:Antibodies, Helminth; D005620:Freund's Adjuvant