Role of Agrobacterium virB genes in transfer of T complexes and RSF1010.
Nonpolar virB mutants of Agrobacterium tumefaciens were tested for RSF1010 mobilization and extracellular complementation. virB2 to virB11 were essential for transfer in both assays. virB1 was essential only for high frequency transfer of RSF1010 and VirE2. Coordinated transfer of a preassembled T complex is supported by these data and competition studies.
Fullner
K J
KJ
Department of Microbiology, University of Washington, Seattle 98195-7242, USA. kfullner@warren.med.harvard.edu
D016960:Agrobacterium tumefaciens / Q000235:genetics*; D001426:Bacterial Proteins / Q000235:genetics*; D018014:Gene Transfer Techniques; D005798:Genes, Bacterial; D005816:Genetic Complementation Test; D016296:Mutagenesis; D017384:Sequence Deletion; D037521:Virulence Factors
D001426:Bacterial Proteins; D037521:Virulence Factors