Selective actions of a detergent on ligand-gated ion channels expressed in Xenopus oocytes.
Cytoclean a commercially available detergent, has selective actions on ligand-gated ion channels. Cytoclean (0.0005-0.01% v/v) potentiated 50 microM glycine responses in oocytes expressing alpha-2 glycine receptors by 23 +/- 7% to 342 +/- 43%. Cytoclean is composed of five components dissolved in water, but only one reagent, Bio-Soft D-62, modulated responses of oocytes expressing alpha-2 glycine receptors. Bio-Soft D-62 (0.00005-0.001% w/v), potentiated 50 microM glycine responses by 13 +/- 1% to 474 +/- 50%. Bio-Soft D-62 is composed of linear alkylbenzene sulfonate (> 95% C12 chain). The effects of Cytoclean or Bio-Soft D-62 were examined on alpha-1 beta-2 and alpha-1 beta-2 gamma-2L gamma-aminobutyric acidA, gamma-aminobutyric acid rho 1, DL-alpha-amino-3-hydroxy-5-methyl-4-isoxalonepropionic acid, kainate and 5-hydroxytryptamine3 receptors expressed in Xenopus laevis oocytes. Enhancement of gamma-aminobutyric acidA receptor function ranged from approximately 21% to 458% with Cytoclean (0.0001-0.01%), respectively. Bio-Soft D-62 (0.001%) inhibited GABA rho 1 receptor function by approximately 72%. Cytoclean had no effect on 5-hydroxytryptamine3 or GluR6 function, but Cytoclean (0.005% and 0.01%) inhibited GluR3-mediated currents by approximately 21% and approximately 41%, respectively. These results suggest that trace amounts of Cytoclean, such as amounts adhering to glassware, may modulate ion channel function and potentially confound experimental results.
Machu
T K
TK
Department of Pharmacology, Texas Tech University Health Sciences Center, Lubbock, USA. phrtkm@ttuhsc.edu
Mihic
S J
SJ
Dildy-Mayfield
J E
JE
D000818:Animals; D003902:Detergents / Q000494:pharmacology*; D005260:Female; D005998:Glycine / Q000494:pharmacology; D015640:Ion Channel Gating; D007473:Ion Channels / Q000187:drug effects*; D008024:Ligands; D009865:Oocytes; D014982:Xenopus laevis
D003902:Detergents; D007473:Ion Channels; D008024:Ligands; D005998:Glycine