title: Structural characterization of the molten globule and native states of ovalbumin: a 1H NMR study.
abstract: Molecular characteristics of ovalbumin (OVA) in the acidic (pD 3.08, the E-form) and neutral [pD 7.29, the N-form (native form)] regions were studied by measuring effective radii, 1H NMR spectra, spin-echo 1H NMR spectra and cross-relaxation times (TIS) from irradiated to observed protein protons which are particularly sensitive for detection of the mobile segments and/or structural looseness in proteins. 1H NMR spectra did not show significant differences between the N- and E-forms except for the spectral lines in the CH3, epsilon CH2 and aromatic regions. Effective radii and TIS values for main- and side-chains showed 1.08 and 1.5- to 2.0-fold increases on going from the N- to E-forms, respectively. The elongation of TIS values might indicate the appearance of the fluctuating tertiary structure in the E-form. Molecular characteristics of the E-form, inferred from reported far ultraviolet-circular dichroism (UV-CD) spectra in the peptide region, near UV-CD spectra in the aromatic region [Koseki et al. (1988) J. Biochem. 103, 425-430], effective radii and especially elongation of TIS values might indicate that the E-form could be in the molten globule state. The onset of denaturation of OVA using TIS measurements was also studied.
authors.affiliation: Department of Physiology, School of Health Sciences, Fujita Health University, Toyoake, Japan.
authors.forename: M
authors.initials: M
authors.lastname: Sogami
authors.forename: S
authors.initials: S
authors.lastname: Era
authors.forename: T
authors.initials: T
authors.lastname: Koseki
authors.forename: N
authors.initials: N
authors.lastname: Nagai
mesh_terms: D000818:Animals; D055598:Chemical Phenomena; D002627:Chemistry, Physical; D002645:Chickens; D002942:Circular Dichroism; D003903:Deuterium; D006863:Hydrogen-Ion Concentration; D009682:Magnetic Resonance Spectroscopy; D010047:Ovalbumin / Q000737:chemistry*; D011489:Protein Denaturation; D017434:Protein Structure, Tertiary; D014508:Urea
chemical_list: D014508:Urea; D010047:Ovalbumin; D003903:Deuterium