title: Structure of a novel phosphocholine-containing aminoglycoglycerolipid of Mycoplasma fermentans.
abstract: Mycoplasma fermentans is thought to be a pathogen of rheumatoid arthritis or a cofactor of AIDS (acquired immunodeficiency syndrome). To elucidate the possible involvement of membrane constituents in the pathogenesis of these diseases, we studied its lipid components. Several alkali labile glycophospholipids were detected and named glycoglycerophospholipids (GGPLs). Previously, we purified and determined the structure of one of them as 6'-O-phosphocholine-alpha-glucopyranosyl-(1'-3)-1,2-diacyl-sn-glycerol (GGPL-I). The present paper describes the purification and structural characterization of GGPL-III, the major GGPL of M. fermentans using 1H-, 13C- and 31P-nuclear magnetic resonance spectroscopy, and mass-spectroscopy as 1"-phosphocholine,2"-amino dihydroxypropane-3"-phospho-6'-alpha-glucopyranosyl-(1'-3)-1,2-dia cyl-glycerol.
authors.affiliation: Department of Microbiology, Tokyo Medical and Dental University, Japan. kmats90820@aol.com
authors.forename: K
authors.initials: K
authors.lastname: Matsuda
authors.forename: I
authors.initials: I
authors.lastname: Ishizuka
authors.forename: T
authors.initials: T
authors.lastname: Kasama
authors.forename: S
authors.initials: S
authors.lastname: Handa
authors.forename: N
authors.initials: N
authors.lastname: Yamamoto
authors.forename: T
authors.initials: T
authors.lastname: Taki
mesh_terms: D006017:Glycolipids / Q000737:chemistry*; D009682:Magnetic Resonance Spectroscopy; D013058:Mass Spectrometry; D016858:Mycoplasma fermentans / Q000737:chemistry*; D010767:Phosphorylcholine / Q000737:chemistry* / Q000302:isolation & purification
chemical_list: D006017:Glycolipids; D010767:Phosphorylcholine