title: NotI linking/jumping clones of human chromosome 3: mapping of the TFRC, RAB7 and HAUSP genes to regions rearranged in leukemia and deleted in solid tumors.
abstract: By applying the 'recognition mask' strategy to 300 mammalian sequences containing NotI sites we demonstrated that 5' ends of genes are highly enriched in NotI sites. A NotI linking clone NL2-252 (D3S1678) containing transferrin receptor (TFRC) gene was used as an initial point for chromosomal jumping. One of the jumping clones, J21-045 traverses 210 kbp and links NL2-252 to NL26 (D3S1632), a NotI linking clone containing highly polymorphic sequences. The TFRC gene was mapped to 3q29, close to the telomeric marker D3S2344, by linkage analysis, a panel of hybrid cell lines, GeneBridge 4 panel and FISH. Clone NLM-007 (D3S4302) was found to contain ras-homologous gene RAB7. By FISH and a panel of hybrid cell lines this gene was mapped to 3q21. This region is of particular interest due to frequent rearrangements in different types of leukemia. Clone L2-081 (D3S4283) containing new member of ubiquitin-specific proteases (HAUSP gene) was localized in 3p21 inspiring further investigation of involvement of this gene in development of lung and renal carcinomas.
authors.affiliation: Microbiology and Tumor Biology Center, Karolinska Institute, Stockholm, Sweden.
authors.forename: V I
authors.initials: VI
authors.lastname: Kashuba
authors.forename: R Z
authors.initials: RZ
authors.lastname: Gizatullin
authors.forename: A I
authors.initials: AI
authors.lastname: Protopopov
authors.forename: R
authors.initials: R
authors.lastname: Allikmets
authors.forename: S
authors.initials: S
authors.lastname: Korolev
authors.forename: J
authors.initials: J
authors.lastname: Li
authors.forename: F
authors.initials: F
authors.lastname: Boldog
authors.forename: K
authors.initials: K
authors.lastname: Tory
authors.forename: V
authors.initials: V
authors.lastname: Zabarovska
authors.forename: Z
authors.initials: Z
authors.lastname: Marcsek
authors.forename: J
authors.initials: J
authors.lastname: Sumegi
authors.forename: G
authors.initials: G
authors.lastname: Klein
authors.forename: E R
authors.initials: ER
authors.lastname: Zabarovsky
authors.forename: L
authors.initials: L
authors.lastname: Kisselev
mesh_terms: D002874:Chromosome Mapping; D002893:Chromosomes, Human, Pair 3; D003001:Cloning, Molecular; D010450:Endopeptidases / Q000235:genetics*; D019204:GTP-Binding Proteins / Q000235:genetics*; D015723:Gene Library; D015321:Gene Rearrangement; D015894:Genome, Human; D006801:Humans; D007938:Leukemia / Q000235:genetics*; D008969:Molecular Sequence Data; D009369:Neoplasms / Q000235:genetics*; D011990:Receptors, Transferrin / Q000235:genetics*; D043222:Ubiquitin Thiolesterase; D000076084:Ubiquitin-Specific Peptidase 7; D020691:rab GTP-Binding Proteins; D000090802:rab7 GTP-Binding Proteins
chemical_list: D011990:Receptors, Transferrin; D000090802:rab7 GTP-Binding Proteins; C000716463:rab7 GTP-binding proteins, human; D010450:Endopeptidases; C464569:USP7 protein, human; D043222:Ubiquitin Thiolesterase; D000076084:Ubiquitin-Specific Peptidase 7; D019204:GTP-Binding Proteins; D020691:rab GTP-Binding Proteins