Distribution and accumulation of storage protein-1 in ovary of Hyphantria cunea Drury.
Storage protein-1 (SP-1) is a major storage protein found in the hemolymph and fat body of Hyphantria cunea. In this study, the uptake and accumulation of SP-1 into the ovary of H. cunea was investigated using biochemical and immunocytochemical methods. SP-1 in H. cunea has a high methionine content (4.6%) but is not female-specific, like other high methionine storage proteins. In the 6-day-old pupal ovary, SP-1 was detectable in trace amounts but accumulated to significant levels toward the end of the pupal stage. After adult emergence, SP-1 rapidly decreased in the ovarian follicles and remained low in the egg. This suggest that SP-1 is either extensively modified or degraded, causing a loss of its antigenic property in the ovary after adult emergence. During vitellogenesis, SP-1 is present in the hemolymph and penetrates through the tunica propria to reach the perioocytic space. From there, SP-1 is incorporated into yolk bodies. These results clearly show that SP-1 is taken up by the developing oocyte. Its disappearance suggests that SP-1 might be an amino acid reservoir for providing precursors for egg formation, in contrast to yolk proteins, which are utilized during postembryonic development.
Seo
S J
SJ
Department of Biology, Gyeongsang National University, Chinju, Korea. sookjae@gshp.gsnu.ac.kr
Kang
Y J
YJ
Cheon
H M
HM
Kim
H R
HR
D000596:Amino Acids / Q000032:analysis; D000818:Animals; D005260:Female; D007150:Immunohistochemistry; D019476:Insect Proteins / Q000737:chemistry / Q000378:metabolism*; D009036:Moths / Q000378:metabolism*; D009865:Oocytes / Q000378:metabolism; D010053:Ovary / Q000378:metabolism*
D000596:Amino Acids; D019476:Insect Proteins; C041137:storage proteins, Insecta