Cloning and characterization of the murine homologue of the rat/human MRC OX-2 gene.
A 350 bp amplicon, obtained by PCR-select subtractive hybridization from RNA derived from mesenteric lymph nodes (MLN) cells from mice pre-immunized with allogeneic lymphocytes 36hrs prior to receiving donor-specific skin grafts, and showing > 98% homology with a published sequence for the rat MRC OX-2 gene, was used as a hybridization probe to screen a cDNA library constructed from adult mouse MLN treated in the same fashion. Several clones were identified which, on DNA sequence analysis, predicted a 218 amino acid protein showing significant homology with the rat and human MRC OX-2 gene product.
Chen
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Z
The Toronto Hospital, Dept. Surgery, University of Toronto, Ontario, Canada.
Zeng
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H
Gorczynski
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RM
D019264:Adoptive Transfer; D000595:Amino Acid Sequence; D000818:Animals; D015703:Antigens, CD; D000954:Antigens, Surface / Q000235:genetics*; D001483:Base Sequence; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics*; D005796:Genes / Q000235:genetics; D006801:Humans; D008198:Lymph Nodes; D051379:Mice; D008809:Mice, Inbred C3H; D008969:Molecular Sequence Data; D051381:Rats; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D016038:Skin Transplantation
D015703:Antigens, CD; D000954:Antigens, Surface; D018076:DNA, Complementary; C042739:antigens, CD200