title: Immunochemical localization of the small leucine-rich proteoglycan lumican in human predentine and dentine.
abstract: The immunocalization of the small interstitial leucine-rich keratan sulphate proteoglycan lumican was studied in human teeth using a polyclonal antibody towards lumican and a standard indirect peroxidase technique. There was intense labelling for lumican in predentine, with immunopositivity also observed in and around the tubules, around the odontoblasts and in the pulp with a fibrillar distribution. The previous reported ability of lumican to control collagen fibrillogenesis and it putative function in collagen spacing suggest potential roles for lumican in predentine in relation to mineralization.
authors.affiliation: Department of Basic Dental Science, University of Wales College of Medicine, Heath Park, Cardiff, U.K.
authors.forename: R C
authors.initials: RC
authors.lastname: Hall
authors.forename: G
authors.initials: G
authors.lastname: Embery
authors.forename: D
authors.initials: D
authors.lastname: Lloyd
mesh_terms: D000293:Adolescent; D000328:Adult; D000906:Antibodies; D002648:Child; D011508:Chondroitin Sulfate Proteoglycans / Q000032:analysis*; D003094:Collagen / Q000648:ultrastructure; D004396:Coloring Agents; D003782:Dental Pulp / Q000648:ultrastructure; D003804:Dentin / Q000648:ultrastructure*; D006801:Humans; D007124:Immunoenzyme Techniques; D007150:Immunohistochemistry; D007632:Keratan Sulfate / Q000032:analysis*; D007930:Leucine / Q000032:analysis; D000071234:Lumican; D009804:Odontoblasts / Q000648:ultrastructure; D014074:Tooth Calcification
chemical_list: D000906:Antibodies; D011508:Chondroitin Sulfate Proteoglycans; D004396:Coloring Agents; C000604306:LUM protein, human; D000071234:Lumican; D003094:Collagen; D007632:Keratan Sulfate; D007930:Leucine