title: Mass spectrometric identification of phosphorylated vasostatin II, a chromogranin A-derived protein fragment (1-113).
abstract: Vasostatin II, an N-terminal chromogranin A-derived protein (CGA1-113), was purified from bovine chromaffin granule lysate and characterized by electrospray mass spectrometry (ES/MS) as being partially phosphorylated. The phosphorylation site was determined to be at the Ser81 position by mass spectrometric peptide mapping and tandem mass spectrometric analysis. This phosphorylation site is close to the processing site (...QKK78HSS(p)81...) yielding vasostatin I, an N-terminal CGA-derived peptide comprising residues 1-76, suggesting that phosphorylation at Ser81 is involved in the formation of vasostatin I in chromaffin cells.
authors.affiliation: Department of Pharmaceutical Sciences, University of Antwerp (UIA), Belgium.
authors.forename: X
authors.initials: X
authors.lastname: Zhang
authors.forename: L
authors.initials: L
authors.lastname: Dillen
authors.forename: S H
authors.initials: SH
authors.lastname: Bauer
authors.forename: W
authors.initials: W
authors.lastname: Van Dongen
authors.forename: F
authors.initials: F
authors.lastname: Liang
authors.forename: M
authors.initials: M
authors.lastname: Przybylski
authors.forename: E
authors.initials: E
authors.lastname: Esmans
authors.forename: W P
authors.initials: WP
authors.lastname: De Potter
authors.forename: M
authors.initials: M
authors.lastname: Claeys
mesh_terms: D000469:Alkaline Phosphatase / Q000378:metabolism; D000595:Amino Acid Sequence; D000818:Animals; D002417:Cattle; D019439:Chromaffin Cells / Q000737:chemistry; D002850:Chromatography, Gel; D053379:Chromogranin A; D002864:Chromogranins / Q000737:chemistry* / Q000302:isolation & purification / Q000378:metabolism*; D013058:Mass Spectrometry; D008969:Molecular Sequence Data; D010446:Peptide Fragments / Q000737:chemistry* / Q000302:isolation & purification / Q000378:metabolism; D010449:Peptide Mapping; D010748:Phosphopeptides / Q000032:analysis / Q000737:chemistry; D010766:Phosphorylation; D010768:Phosphoserine / Q000032:analysis; D011499:Protein Processing, Post-Translational; D017421:Sequence Analysis; D014357:Trypsin / Q000378:metabolism
chemical_list: D053379:Chromogranin A; D002864:Chromogranins; D010446:Peptide Fragments; D010748:Phosphopeptides; C102106:vasostatin II; D010768:Phosphoserine; D000469:Alkaline Phosphatase; D014357:Trypsin