Differential regulation of genes encoding synaptic proteins by the Oct-2 transcription factor.
In order to investigate the effect of the Oct-2 POU family transcription factor on the regulation of genes encoding synaptic proteins, we have used cell lines in which the level of Oct-2 has been greatly reduced using an antisense approach. The reduced Oct-2 level results in enhanced expression of SNAP-25 and synapsin I, indicating that the genes encoding these proteins are normally repressed by Oct-2 in neuronal cells. In contrast, no alteration was observed in the levels of the synaptic proteins, synaptophysin and synaptotagmin. Although the neuronal forms of Oct-2 can repress the synapsin I promoter in co-transfection experiments, indicating that they have a direct effect on the expression of this gene, they have no effect on the activity of the SNAP-25 promoter, indicating that the effect of Oct-2 on this gene is likely to be indirect. These effects are discussed in terms of the differential effect of Oct-2 and the related POU family transcription factor Brn-3a, on the promoters of genes encoding different synaptic proteins.
Deans
Z C
ZC
Department of Molecular Pathology, University College Medical School, London, UK.
Dawson
S J
SJ
Kilimann
M W
MW
Wallace
D
D
Wilson
M C
MC
Latchman
D S
DS
D000818:Animals; D002135:Calcium-Binding Proteins; D002460:Cell Line; D006224:Cricetinae; D004268:DNA-Binding Proteins / Q000096:biosynthesis / Q000378:metabolism*; D005786:Gene Expression Regulation; D008562:Membrane Glycoproteins / Q000096:biosynthesis; D008565:Membrane Proteins; D009419:Nerve Tissue Proteins / Q000096:biosynthesis*; D009474:Neurons; D011401:Promoter Regions, Genetic; D011994:Recombinant Proteins / Q000096:biosynthesis; D013569:Synapses / Q000378:metabolism*; D016704:Synapsins / Q000096:biosynthesis*; D016708:Synaptophysin / Q000096:biosynthesis; D050825:Synaptosomal-Associated Protein 25; D050857:Synaptotagmins; D014157:Transcription Factors / Q000096:biosynthesis / Q000378:metabolism*; D014162:Transfection
D002135:Calcium-Binding Proteins; D004268:DNA-Binding Proteins; D008562:Membrane Glycoproteins; D008565:Membrane Proteins; D009419:Nerve Tissue Proteins; D011994:Recombinant Proteins; D016704:Synapsins; D016708:Synaptophysin; D050825:Synaptosomal-Associated Protein 25; D014157:Transcription Factors; D050857:Synaptotagmins