title: Allergen-induced airway reactions in atopic asthmatics correlate with allergen-specific IL-5 response by BAL cells.
abstract: Allergen-specific cytokine responses in the airways are thought to play a critical role in the pathogenesis of atopic asthma. This study examined whether there is a quantitative difference in bronchoalveolar lavage (BAL) cell allergen-induced IL-5 production between atopic subjects with and without asthma which may relate to a difference in airway response induced by allergen exposure. Twelve atopic asthmatics (AA), nine atopic non-asthmatics (AN) and 10 normal controls (N) underwent inhalation challenge with house dust mite allergen (HDM) extract. AA differed from AN in having late airway reactions (LAR) after HDM inhalation (P < 0.01), which correlated with an increased percentage of BAL eosinophils and increased BAL cell IL-5 production after in vivo or in vitro HDM challenge for the AA group (P < 0.01). IL-5 production by PBMC from both atopic groups was elevated with HDM stimulation in vitro, but AA again had a higher level under baseline conditions than AN (P < 0.02). Furthermore, there was a greater effect of BAL fluid from AA on ECP release by eosinophils compared to that for AN (P < 0.01). These findings suggest that increased IL-5 production in atopic asthmatic airways contributes to the increased physiological response to allergen inhalation, by modulating local eosinophil recruitment and activation.
authors.affiliation: Department of Respiratory Medicine, Alfred Hospital, Prahran, Victoria, Australia.
authors.forename: C
authors.initials: C
authors.lastname: Tang
authors.forename: J M
authors.initials: JM
authors.lastname: Rolland
authors.forename: C
authors.initials: C
authors.lastname: Ward
authors.forename: B
authors.initials: B
authors.lastname: Quan
authors.forename: E H
authors.initials: EH
authors.lastname: Walters
mesh_terms: D000328:Adult; D000485:Allergens / Q000008:administration & dosage*; D001249:Asthma / Q000175:diagnosis / Q000276:immunology*; D016535:Bronchial Hyperreactivity / Q000175:diagnosis / Q000276:immunology; D001985:Bronchial Provocation Tests / Q000379:methods*; D001992:Bronchoalveolar Lavage Fluid / Q000166:cytology / Q000276:immunology*; D001999:Bronchoscopy; D018791:CD4 Lymphocyte Count; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D005434:Flow Cytometry; D006632:Histamine / Q000008:administration & dosage; D006801:Humans; D006969:Hypersensitivity, Immediate / Q000276:immunology*; D015848:Interleukin-5 / Q000096:biosynthesis*; D008297:Male; D008875:Middle Aged; D012016:Reference Values
chemical_list: D000485:Allergens; D015848:Interleukin-5; D006632:Histamine