Structural analysis and molecular modeling of two anti-trichosanthin IgE clones from phage antibody library.
Recently we constructed a murine IgE phage surface display library and screened out two IgE (Fab) clones with specific binding activity to Trichosanthin (TCS). In this work, the V epsilon and V kappa genes of the two clones were sequenced and their putative germline gene usages were studied. On the basis of the known 3D structure of Trichosanthin and antibody, molecular modeling was carried out to study the antigen-antibody interaction. The possible antigenic determinant sites on the surface of TCS recognized by both the clones were analyzed, and the reaction forces between TCS and two Fab fragments were also analyzed respectively.
Li
Z D
ZD
Shanghai Institute of Cell Biology, Chinese Academy of Sciences, China.
Yuan
Y R
YR
Yeh
M
M
D000818:Animals; D017104:Bacteriophage M13 / Q000235:genetics / Q000276:immunology*; D003001:Cloning, Molecular; D015723:Gene Library; D006860:Hydrogen Bonding; D007073:Immunoglobulin E / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D007140:Immunoglobulin Fab Fragments / Q000378:metabolism; D007135:Immunoglobulin Variable Region / Q000737:chemistry / Q000235:genetics; D051379:Mice; D008958:Models, Molecular; D017422:Sequence Analysis, DNA; D015978:Trichosanthin / Q000276:immunology* / Q000378:metabolism
D007140:Immunoglobulin Fab Fragments; D007135:Immunoglobulin Variable Region; D007073:Immunoglobulin E; D015978:Trichosanthin