MAP2, synaptophysin immunostaining in rat brain and behavioral modifications after cerebral postischemic reperfusion.
Plasticity in the central nervous system after cerebral ischemia is a controversial issue; focal cerebral ischemia produces an area of infarction that is surrounded by neurons that may respond to nearby damage by creating new synapses. In the present study the expression of the postsynaptic microtubule-associated protein 2 (MAP2) and the presynaptic marker protein, synaptophysin, was investigated by immunocytochemical techniques in the CA1 sector of hippocampus and in cerebellum of rats made ischemic by bilateral clamping of common carotid arteries and reperfused for 7 and 30 days. In addition, ischemia-induced behavioral alterations were also evaluated after 7 and 30 days of reperfusion. The present study demonstrates a decreased postsynaptic MAP2 immunoreactivity, representative of neuronal loss, particularly in CA1 sector of hippocampus and in cerebellum of ischemic rats reperfused for 7 days. After 30 days of reperfusion, MAP2 immunostaining was similar to control. In the same brain sections an increased presynaptic synaptophysin immunoreactivity has been observed only after 30 days of reperfusion. These data suggest compensatory regenerative changes associated with synaptic remodelling and are supported by behavioral recovery observed under the same experimental conditions.
Martinez
G
G
Institute of Human Anatomy, University of Catania, Italy.
Di Giacomo
C
C
Carnazza
M L
ML
Sorrenti
V
V
Castana
R
R
Barcellona
M L
ML
Perez-Polo
J R
JR
Vanella
A
A
D000818:Animals; D001362:Avoidance Learning / Q000502:physiology; D001522:Behavior, Animal / Q000502:physiology*; D001923:Brain Chemistry / Q000502:physiology*; D002525:Cerebellar Cortex / Q000473:pathology; D002531:Cerebellum / Q000473:pathology; D002560:Cerebrovascular Circulation / Q000502:physiology; D006624:Hippocampus / Q000473:pathology; D007150:Immunohistochemistry; D008297:Male; D008869:Microtubule-Associated Proteins / Q000378:metabolism*; D009043:Motor Activity / Q000502:physiology; D004856:Postural Balance / Q000502:physiology; D051381:Rats; D017208:Rats, Wistar; D015427:Reperfusion Injury / Q000378:metabolism* / Q000523:psychology*; D016708:Synaptophysin / Q000378:metabolism*
D008869:Microtubule-Associated Proteins; D016708:Synaptophysin