Early ultrastructural changes in the dorsal mucosa of rat tongue after irradiation, with special reference to the microvasculature.
OBJECTIVE
To clarify the acute effects of irradiation on the ultrastructural conformation of the dorsal mucosa of the rat tongue, with special reference to the changes in microvasculature.


METHODS
The proboscis of seventy rats were irradiated. The animals were then perfusion-fixed, followed by India ink-injection or resin casting at 3 to 7 days after irradiation. The bulk, frozen sections, or plastic embedded sections of the treated rat tongues were examined by light and electron microscopy.


RESULTS
In the dorsal epithelium of the rat tongue, multi-nucleated cells appeared in the basal layer at 3 days after irradiation. At day 5, the thickness of the epithelial layer and connective tissue papillae decreased dramatically, concomitant with the shortening of the capillary loops. At day 7, lingual papillae and connective tissue papillae disappeared, leaving dissociated epithelial cells and numerous neutrophils migrating throughout the tissue. Subepithelial blood vessels displayed drastic dilation with a number of neutrophils adhering to the endothelial surface, but without ultrastructural abnormalities in its cellular components.


CONCLUSIONS
Early changes in the dorsal mucosa of the irradiated rat tongue were limited to the basal epithelial cells, leading to a total disruption of the epithelial layer. Atrophic changes of the capillary loops is due to the loss of the connective tissue papillae. Dilation and conformational changes of the subepithelial capillaries appear to result from the inflammatory reaction, taking place secondarily to the loss of the epithelial barrier of the irradiated tongue. A difference in radiosensitivity among the epithelial, endothelial, and mesenchymal cell components of the rat tongue in vivo is suggested.
Obinata
K
K
Department of Oral and Maxillofacial Radiology, Niigata University School of Dentistry, Japan.
Ohshima
H
H
Takano
Y
Y
Ito
J
J
D000818:Animals; D002244:Carbon; D002455:Cell Division / Q000528:radiation effects; D002465:Cell Movement / Q000528:radiation effects; D004396:Coloring Agents; D004195:Disease Models, Animal; D004730:Endothelium, Vascular / Q000528:radiation effects / Q000648:ultrastructure; D005500:Follow-Up Studies; D008264:Macrophages / Q000528:radiation effects / Q000648:ultrastructure; D008297:Male; D008833:Microcirculation / Q000528:radiation effects / Q000648:ultrastructure; D008854:Microscopy, Electron; D009092:Mucous Membrane / Q000098:blood supply / Q000528:radiation effects / Q000648:ultrastructure; D009504:Neutrophils / Q000528:radiation effects / Q000648:ultrastructure; D011833:Radiation Injuries, Experimental / Q000473:pathology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D014059:Tongue / Q000098:blood supply / Q000528:radiation effects / Q000648:ultrastructure*
D004396:Coloring Agents; C028433:chinese ink; D002244:Carbon