Apoptosis and cell culture technology.
The importance of apoptosis in cell culture has now been widely recognised. Recent work has shown that this process is widely manifested during the in vitro cultivation of commercially important mammalian cell lines. In this review I summarise what is now known of the characteristics, significance and regulatory mechanisms of apoptosis. As the process of cell proliferation and cell death are now considered intimately related, particular attention is paid to highlight the progress and opportunities in the field of cell culture engineering. The strategies that have been undertaken to prevent the induction of apoptosis in cell culture and those which have been suggested as possibilities to improve culture productivity through the apoptosis route are discussed with given examples.
al-Rubeai
M
M
Centre for Biochemical Engineering, School of Chemical Engineering, University of Birmingham, UK.
D000818:Animals; D017209:Apoptosis / Q000187:drug effects / Q000235:genetics / Q000502:physiology; D018929:Cell Culture Techniques / Q000379:methods*; D018797:Cell Cycle Proteins / Q000502:physiology; D002470:Cell Survival; D003001:Cloning, Molecular; D053938:DNA Fragmentation; D005434:Flow Cytometry; D006801:Humans; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000502:physiology; D014162:Transfection
D018797:Cell Cycle Proteins; D019253:Proto-Oncogene Proteins c-bcl-2