title: Intracellular Ca2+ signals in Dictyostelium chemotaxis are mediated exclusively by Ca2+ influx.
abstract: We measured folate- and cAMP-induced changes in cytoplasmic free calcium concentration ([Ca2+]i) using recombinant aequorin reconstituted in living Dictyostelium cells with coelenterazine-h. The resulting semi-synthetic protein displayed increased sensitivity to Ca2+ allowing accurate measurement of chemoattractant-induced transients at low resting levels. Both folate- and cAMP-induced Ca2+ responses were developmentally regulated, exhibited remarkably similar kinetics and were dependent on the relative rather than the absolute magnitude of increases in attractant concentration. They began after a short delay of 5-10 seconds, leading to a maximum increase in cytosolic calcium concentration after approximately 25 seconds and a return to basal level within approximately 60 seconds after stimulation. Responses elicited by the two chemoattractants were dose-dependent and saturated between 4 and 20 microM. They depended on the presence of free extracellular calcium ions and were inhibited in a concentration-dependent manner between 10(-4) and 10(-5) M. In accordance with 45Ca2+-uptake measurements by Milne and Coukell (J. Cell Biol. (1991) 112, 103-110), both responses were also completely inhibited by 15 microM Ruthenium Red, 15 microM carbonylcyanide m-chlorophenyl-hydrazone (CCCP) and 500 microM gadolinium ions. Under conditions that prohibited influx of Ca2+ from the extracellular medium there were no detectable changes in [Ca2+]i that could be related to a separate release of the ion from intracellular stores. Together, these results show that the Ca2+ signals involved in chemotaxis correlate temporally with actin depolymerization (not polymerization) and are mediated by Ca2+ influx, not IP3-mediated intracellular release.
authors.affiliation: School of Microbiology, La Trobe University, Bundoora, VIC, Australia.
authors.forename: T
authors.initials: T
authors.lastname: Nebl
authors.forename: P R
authors.initials: PR
authors.lastname: Fisher
mesh_terms: D000818:Animals; D002118:Calcium / Q000502:physiology*; D002633:Chemotaxis / Q000502:physiology*; D000242:Cyclic AMP / Q000502:physiology; D004023:Dictyostelium / Q000166:cytology / Q000502:physiology*; D015398:Signal Transduction
chemical_list: D000242:Cyclic AMP; D002118:Calcium