Immunomodulatory activity of oligopeptides related to interleukin 1 receptor antagonist sequence.
We examined the immunomodulatory properties of peptides from interleukin 1 receptor antagonist (IL-1Ra) with regard to the humoral (plaqueforming cells-PFC) and cellular (delayed type hypersensitivity-DTH) immune response and GvH reaction. It was found that peptide RKSSK (II) from the N-terminal part of IL-1Ra, although inactive with regard to the inhibition of IL-1-IL-1 receptor interaction, reduces immune response in a manner similar to cyclosporin A (DTH, PFC in vivo). Peptide GRKSSK (III) was even more potent, whereas peptides from respective fragment of mouse IL-1Ra were weaker immunosuppressants than II. Peptide VTKFYF (VII) from the C-terminal part of IL-1Ra, very active as IL-1 inhibitor, and its analog VIII with Asp residue, characteristic for IL-1, instead of Lys from IL-1Ra, showed only limited activity despite the previously observed competition with IL-1 for the cellular receptor. Thus, no correlation between the inhibitory and immunomodulatory properties of peptides derived from IL-1Ra was observed.
Kluczyk
A
A
Faculty of Chemistry, University of Wrocław, Poland.
Siemion
I Z
IZ
Słoń-Usakiewicz
J J
JJ
Wieczorek
Z
Z
D000276:Adjuvants, Immunologic / Q000008:administration & dosage / Q000138:chemical synthesis / Q000502:physiology*; D000595:Amino Acid Sequence; D000818:Animals; D000917:Antibody Formation / Q000187:drug effects; D004306:Dose-Response Relationship, Immunologic; D004912:Erythrocytes / Q000276:immunology; D005260:Female; D006087:Graft vs Host Reaction / Q000187:drug effects; D006462:Hemolytic Plaque Technique; D006968:Hypersensitivity, Delayed / Q000209:etiology; D007274:Injections, Intraperitoneal; D053590:Interleukin 1 Receptor Antagonist Protein; D051379:Mice; D008807:Mice, Inbred BALB C; D009842:Oligopeptides / Q000008:administration & dosage / Q000138:chemical synthesis / Q000276:immunology*; D017472:Receptors, Interleukin-1 / Q000037:antagonists & inhibitors*; D012756:Sheep / Q000276:immunology; D012795:Sialoglycoproteins / Q000276:immunology*
D000276:Adjuvants, Immunologic; C507764:Il1rn protein, mouse; D053590:Interleukin 1 Receptor Antagonist Protein; D009842:Oligopeptides; D017472:Receptors, Interleukin-1; D012795:Sialoglycoproteins