Action of furosemide on GABA- and glycine currents in rat septal cholinergic neurons in culture.
The action of furosemide on GABA- and glycine-induced currents in rat septal cholinergic neurons in culture was examined by use of the whole-cell voltage-clamp technique. Furosemide inhibited the peak amplitude of either GABA or glycine current in a dose-dependent manner; its half-maximal concentration for them was some 2 and 1 mM, respectively. Furosemide at a concentration of 1 mM depressed the maximal peak amplitude of the dose-response curve for either GABA or glycine to about 70% of control. These results indicate that furosemide inhibits in a noncompetitive fashion GABA and glycine receptor-channels in septal neurons.
Kumamoto
E
E
Department of Physiology, Saga Medical School, Japan. kumamoto@smsnet.saga-med.ac.jp
Murata
Y
Y
D000818:Animals; D001692:Biological Transport / Q000187:drug effects; D001923:Brain Chemistry / Q000187:drug effects; D002799:Cholinergic Fibers / Q000737:chemistry / Q000187:drug effects / Q000502:physiology; D004232:Diuretics / Q000494:pharmacology*; D005665:Furosemide / Q000494:pharmacology*; D005998:Glycine / Q000493:pharmacokinetics*; D009474:Neurons / Q000737:chemistry / Q000187:drug effects / Q000502:physiology; D018408:Patch-Clamp Techniques; D051381:Rats; D011963:Receptors, GABA-A / Q000378:metabolism; D018009:Receptors, Glycine / Q000378:metabolism; D012686:Septal Nuclei / Q000166:cytology*; D005680:gamma-Aminobutyric Acid / Q000493:pharmacokinetics*
D004232:Diuretics; D011963:Receptors, GABA-A; D018009:Receptors, Glycine; D005680:gamma-Aminobutyric Acid; D005665:Furosemide; D005998:Glycine