Allyl sulfides from garlic suppress the in vitro proliferation of human A549 lung tumor cells.
The present studies compared the antiproliferative effects of diallyl trisulfide (DATS) and diallyl disulfide (DADS) on cultured human neoplastic (A549) and nonneoplastic (MRC-5) lung cells. Addition of 10 microM DATS reduced A549 growth by 47%, whereas 10 microM DADS decreased growth by only 20%. DATS treatment (10 microM) did not alter MRC-5 cell growth. DATS (10 microM) caused a marked and progressive increase in intracellular Ca2+ in A549 cells during the first four hours after treatment. Intracellular Ca2+ in A549 cells exposed to DATS returned to near control levels within one hour after refeeding complete medium without DATS. Exposure to 1 microM DATS for 24 hours significantly induced apoptosis, as indicated by increased DNA fragmentation. The ability of DATS and DADS to suppress neoplastic growth is consistent with increasing evidence that several garlic components have anticarcinogenic and antitumorigenic properties.
Sakamoto
K
K
Department of Nutrition, Pennsylvania State University, University Park 16802, USA.
Lawson
L D
LD
Milner
J A
JA
D000498:Allyl Compounds / Q000494:pharmacology*; D016587:Antimutagenic Agents / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects; D002118:Calcium / Q000378:metabolism; D002455:Cell Division / Q000187:drug effects / Q000502:physiology; D053938:DNA Fragmentation / Q000187:drug effects; D004220:Disulfides / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D005737:Garlic / Q000737:chemistry*; D006801:Humans; D008168:Lung / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D008175:Lung Neoplasms / Q000378:metabolism / Q000473:pathology* / Q000517:prevention & control; D010946:Plants, Medicinal; D013440:Sulfides / Q000494:pharmacology*; D013997:Time Factors; D014407:Tumor Cells, Cultured
D000498:Allyl Compounds; D016587:Antimutagenic Agents; D004220:Disulfides; D013440:Sulfides; C042577:diallyl trisulfide; C028009:diallyl disulfide; D002118:Calcium