title: Local hypothermia protects the retina from ischaemic injury in vitrectomy.
abstract: OBJECTIVE
  Hypothermic irrigating solutions were used during vitrectomy in pressure induced ischaemic eyes so that their effects on retinal function and histological changes could be investigated.
  
  
  METHODS
  After anaesthetised albino rabbits underwent closed vitrectomy, their vitreous cavities were continuously irrigated for 30 minutes at a perfusion pressure of 140 mm Hg. The rabbits were divided into three groups according to their intraocular perfusion temperatures--8 degrees C, 22 degrees C, and 38 degrees C. Electroretinograms were taken before and after irrigation. Glutamate levels in the vitreous were examined after irrigation. Eyes were enucleated on the seventh postoperative day and examined histologically.
  
  
  RESULTS
  On the seventh postoperative day, the recovery rate of a-wave amplitudes was significantly lower in the 38 degrees C group than in the 8 degrees C group, and that of b-wave amplitudes was significantly lower in the 38 degrees C group than in either the 8 degrees C or 22 degrees C group. Retinal damage in the 38 degrees C group revealed more severe histological impairment than in either the 8 degrees C or 22 degrees C group. Oedema of the inner retinal layer was significant in both the 22 degrees C and 38 degrees C groups. Glutamates reached peak values 30 minutes after the end of ischaemia in the 38 degrees C group. However, no significant glutamate increases were detected 15 to 60 minutes after ischaemia in either the 8 degrees C or 22 degrees C group.
  
  
  CONCLUSIONS
  Local hypothermia during vitrectomy in acute ischaemic eyes appears to decrease retinal damage.
authors.affiliation: Department of Ophthalmology, Nagoya City University Medical School, Japan.
authors.forename: K
authors.initials: K
authors.lastname: Tamai
authors.forename: E
authors.initials: E
authors.lastname: Toumoto
authors.forename: A
authors.initials: A
authors.lastname: Majima
mesh_terms: D000818:Animals; D004596:Electroretinography; D007036:Hypothermia, Induced / Q000379:methods*; D007511:Ischemia / Q000517:prevention & control*; D009883:Ophthalmic Solutions / Q000008:administration & dosage; D011183:Postoperative Complications / Q000517:prevention & control*; D011817:Rabbits; D012171:Retinal Vessels; D012996:Solutions; D007507:Therapeutic Irrigation; D014821:Vitrectomy / Q000009:adverse effects* / Q000379:methods
chemical_list: D009883:Ophthalmic Solutions; D012996:Solutions