Hydrophobicity and outer membrane proteins of Shigella dysenteriae type 1 after treatment with subinhibitory concentrations of aminoglycosides.
Hydrophobicity and profiles of outer membrane proteins of Shigella dysenteriae type 1 after treatment with subinhibitory concentrations (1/2 or 1/4 of the MIC) of aminoglycosides were studied. The antimicrobial activity of the antibiotics tested was 3.12 mg/L (amikacin, tobramycin) and 6.25 mg/L (gentamicin). The hydrophobicity of the cell surface of S. dysenteriae type 1 was decreased after exposure to all aminoglycosides at a concentration of 1/2 of the MICs; 1/4 of the MICs of the antibiotics did not affect bacterial aggregation in the presence of ammonium sulfate. SDS-polyacrylamide gel electrophoresis showed that the profiles of outer membrane proteins of the strain treated with aminoglycosides at both subinhibitory concentrations were not changed as compared to the control.
Hostacká
A
A
Institute of Preventive and Clinical Medicine, Bratislava, Slovak Republic.
Karelová
E
E
D000583:Amikacin / Q000008:administration & dosage / Q000494:pharmacology*; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D001425:Bacterial Outer Membrane Proteins / Q000737:chemistry*; D002473:Cell Wall / Q000737:chemistry / Q000187:drug effects; D055598:Chemical Phenomena; D002627:Chemistry, Physical; D004591:Electrophoresis, Polyacrylamide Gel; D005839:Gentamicins / Q000008:administration & dosage / Q000494:pharmacology*; D012762:Shigella dysenteriae / Q000187:drug effects* / Q000378:metabolism; D014031:Tobramycin / Q000008:administration & dosage / Q000494:pharmacology*
D000900:Anti-Bacterial Agents; D001425:Bacterial Outer Membrane Proteins; D005839:Gentamicins; D000583:Amikacin; D014031:Tobramycin