Comparison of six sets of PCR primers from two different genomic regions for amplification of GB virus C/hepatitis G virus RNA.
Forty-four clinical samples positive for GB virus C (GBV-C)/hepatitis G virus (HGV) were tested with six primer sets, four from the 5' untranslated region (5'-UTR) and two from the NS5a genomic region. Two of the 5'-UTR primer sets, when used in a single-round 60-cycle PCR, detected between 86.4 and 97.7% of the positive samples, while two different sets from the same area, when used in a nested PCR, amplified between 97.7 and 100% of the positive specimens. Both sets from the NS5a region, when used in a single-round PCR, detected 95.5% of the GBV-C/HGV-positive samples. Parallel testing with two PCR sets, one from the 5'-UTR and a second from NS5a, may eliminate false-negative results attributable to the genetic heterogeneity of the virus.
Andonov
A
A
Laboratory for Viral Hepatitis, Bureau of Microbiology, Health Canada, Ottawa, Ontario. Anton_Andonov@inet.hwc.ca
Sauder
C
C
Jacobsen
H
H
Chaudhary
R
R
D017931:DNA Primers; D018067:Flaviviridae / Q000235:genetics*; D016174:Hepacivirus / Q000235:genetics*; D006801:Humans; D016133:Polymerase Chain Reaction; D012367:RNA, Viral / Q000032:analysis*
D017931:DNA Primers; D012367:RNA, Viral