title: On the antigenic determinants of the lipopolysaccharides of Vibrio cholerae O:1, serotypes Ogawa and Inaba.
abstract: Monoclonal, murine IgG1s S-20-4, A-20-6, and IgA 2D6, directed against Vibrio cholerae O:1 Ogawa-lipopolysaccharide exhibited the same fine specificities and similar affinities for the synthetic methyl alpha-glycosides of the (oligo)saccharide fragments mimicking the Ogawa O-polysaccharide (O-PS). They did not react with the corresponding synthetic fragments of Inaba O-PS. IgG1s S-20-4 and A-20-6 have absolute affinity constants for synthetic Ogawa mono- to hexasaccharides of from approximately 10(5) to approximately 10(6) M-1. For IgG1s S-20-4, A-20-6, and IgA 2D6, the nonreducing terminal residue of Ogawa O-PS is the dominant determinant, accounting for approximately 90% of the maximal binding energy shown by these antibodies. Binding studies of derivatives of the Ogawa monosaccharide and IgGs S-20-4 and A-20-6 revealed that the C-2 O-methyl group fits into a somewhat flexible antibody cavity and that hydrogen bonds involving the oxygen and, respectively, the OH at the 2- and 3-position of the sugar moiety as well as the 2'-position in the amide side chain are required. Monoclonal IgA ZAC-3 and IgG3 I-24-2 are specific for V. cholerae O:1 serotypes Ogawa/Inaba-LPS.1 The former did not show binding with members of either series of the synthetic ligands related to the O-antigens of the Ogawa or Inaba serotypes, in agreement with its reported specificity for the lipid/core region (1). Inhibition studies revealed that the binding of purified IgG3 I-24-2 to Ogawa-LPS might be mediated by a region in the junction of the OPS to the lipid-core region of the LPS. cDNA cloning and analysis of the anti-Ogawa antibodies S-20-4, A-20-6, and 2D6 revealed a very high degree of homology among the heavy chains. Among the light chains, no such homology between S-20-4 and A-20-6 on the one hand, and 2D6 on the other hand, exists. For the anti-Inaba/Ogawa antibodies I-24-2 and ZAC-3, their heavy chains are completely different, with some homology among the light chains.
authors.affiliation: Laboratory of Medicinal Chemistry, NIDDK, National Institutes of Health, Bethesda, Maryland 20892, USA.
authors.forename: J
authors.initials: J
authors.lastname: Wang
authors.forename: S
authors.initials: S
authors.lastname: Villeneuve
authors.forename: J
authors.initials: J
authors.lastname: Zhang
authors.forename: P
authors.initials: P
authors.lastname: Lei
authors.forename: C E
authors.initials: CE
authors.lastname: Miller
authors.forename: P
authors.initials: P
authors.lastname: Lafaye
authors.forename: F
authors.initials: F
authors.lastname: Nato
authors.forename: S C
authors.initials: SC
authors.lastname: Szu
authors.forename: A
authors.initials: A
authors.lastname: Karpas
authors.forename: S
authors.initials: S
authors.lastname: Bystricky
authors.forename: J B
authors.initials: JB
authors.lastname: Robbins
authors.forename: P
authors.initials: P
authors.lastname: Kovác
authors.forename: J M
authors.initials: JM
authors.lastname: Fournier
authors.forename: C P
authors.initials: CP
authors.lastname: Glaudemans
mesh_terms: D000595:Amino Acid Sequence; D000907:Antibodies, Bacterial / Q000235:genetics / Q000276:immunology*; D000911:Antibodies, Monoclonal / Q000235:genetics; D000918:Antibody Specificity; D001667:Binding, Competitive; D003001:Cloning, Molecular; D000939:Epitopes; D007074:Immunoglobulin G / Q000235:genetics / Q000276:immunology; D008024:Ligands; D008969:Molecular Sequence Data; D009005:Monosaccharides / Q000276:immunology; D019081:O Antigens / Q000276:immunology*; D009844:Oligosaccharides / Q000276:immunology; D013045:Species Specificity; D014734:Vibrio cholerae / Q000145:classification / Q000276:immunology*
chemical_list: D000907:Antibodies, Bacterial; D000911:Antibodies, Monoclonal; D000939:Epitopes; D007074:Immunoglobulin G; D008024:Ligands; D009005:Monosaccharides; D019081:O Antigens; D009844:Oligosaccharides