A period of anaerobic exercise increases the accumulation of reactive carbonyl derivatives in the lungs of rats.
It is known that acute physical exercise may have diverse pathophysiological consequences in various organs due to free radical formation. We have investigated whether a period of anaerobic running to exhaustion in rats results in oxidative modification of proteins in the lungs. Six rats of an exercised group (E) ran for two periods of 5 min at a speed of 30 m.min-1 followed by a recovery period of 5 min, and then by a third period of running to exhaustion. Reactive carbonyl derivatives (RCD) were measured by the Western blot technique on lungs of E and control (C) rats. In addition, the activity of glutamine synthetase (GS) was also monitored as marker of oxidative damage to proteins. This investigation revealed significant exercise-induced increases in accumulation of RCD in the lungs of the E group compared with the C group. The RCD signals were visibly stronger in proteins with molecular weight of 55 kDa and 32 kDa. The activity of GS was higher by about 30% in E rats than in C rats. The present data suggest that anaerobic exercise induces protein oxidation in the lungs.
Radák
Z
Z
Laboratory of Exercise Physiology, Institute of Sport and Health Sciences, University of Tsukuba, Tsukuba.
Nakamura
A
A
Nakamoto
H
H
Asano
K
K
Ohno
H
H
Goto
S
S
D000693:Anaerobiosis; D000818:Animals; D015153:Blotting, Western; D005609:Free Radicals; D005974:Glutamate-Ammonia Ligase / Q000378:metabolism; D006835:Hydrazones / Q000378:metabolism*; D008168:Lung / Q000378:metabolism*; D008297:Male; D008970:Molecular Weight; D010084:Oxidation-Reduction; D018384:Oxidative Stress; D005082:Physical Exertion / Q000502:physiology*; D011506:Proteins / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar
D005609:Free Radicals; D006835:Hydrazones; D011506:Proteins; D005974:Glutamate-Ammonia Ligase