title: Differentiation of Campylobacter isolates on the basis of sensitivity to boiling in water as measured by PCR-detectable DNA.
abstract: Differential sensitivity for the release of PCR-detectable genomic DNA upon boiling in water is reported for 45 Campylobacter jejuni and Campylobacter coli strains isolated in Egypt. All of the strains released PCR-detectable DNA when treated with proteinase K and sodium dodecyl sulfate. When DNA was extracted from these strains by boiling in water, nine (20%) of the strains were PCR negative or resistant to boiling, suggesting the presence of boiling-sensitive and boiling-resistant phenotypes.
authors.affiliation: U.S. Naval Medical Research Unit No. 3, Cairo, Egypt. namru3_RSD@centcom.dsaa.osd.mil
authors.forename: Z S
authors.initials: ZS
authors.lastname: Mohran
authors.forename: R R
authors.initials: RR
authors.lastname: Arthur
authors.forename: B A
authors.initials: BA
authors.lastname: Oyofo
authors.forename: L F
authors.initials: LF
authors.lastname: Peruski
authors.forename: M O
authors.initials: MO
authors.lastname: Wasfy
authors.forename: T F
authors.initials: TF
authors.lastname: Ismail
authors.forename: J R
authors.initials: JR
authors.lastname: Murphy
mesh_terms: D017000:Campylobacter coli / Q000145:classification / Q000302:isolation & purification* / Q000502:physiology; D016123:Campylobacter jejuni / Q000145:classification / Q000302:isolation & purification* / Q000502:physiology; D004269:DNA, Bacterial / Q000032:analysis* / Q000302:isolation & purification*; D004534:Egypt; D019286:Endopeptidase K / Q000494:pharmacology; D006361:Heating; D016133:Polymerase Chain Reaction / Q000379:methods*; D012967:Sodium Dodecyl Sulfate / Q000494:pharmacology; D014867:Water
chemical_list: D004269:DNA, Bacterial; D014867:Water; D012967:Sodium Dodecyl Sulfate; D019286:Endopeptidase K