title: Early changes in the phenotypic composition of lymphocytes in the bronchoalveolar lavage of pigs after aerogenic immunization with Pasteurella multocida aerosols.
abstract: The phenotypic composition of bronchoalveolar lymphocytes (BALL) of healthy untreated pigs and of pigs immunized via aerosol with a live Pasteurella multocida mutant was measured cytofluorometrically in comparison to blood lymphocytes (PBL). Cells were analyzed using monoclonal antibodies against porcine IgM, IgA, IgG and SWC3 in single-colour fluorescence and the following antibody combinations for two-colour fluorescence: CD2/SWC1, SWC1/CD25 and CD4/CD8. In nonimmunized pigs, differences between lymphocyte subsets derived from blood and lung were obvious. The numbers of CD2-SWC1+ T-cells, CD25+ activated lymphocytes and IgG+ cells were significantly higher in the bronchoalveolar compartment. Aerosol immunization with P. multocida caused only insignificant changes in the phenotypic composition of PBL whereas the composition of lung-derived lymphocytes changed markedly. The percentage of CD2-SWC1+ lymphocytes and of CD25+ cells coexpressing the SWC1 antigen in BALL increased up to 10 days after aerosol administration, whereas the percentage of all Ig+ cells decreased. These data reflect an early cellular reaction to an aerogenic immunization with a P. multocida aerosol, characterized by an increase in CD2+ T-cells and a T-cell activation.
authors.affiliation: Federal Institute for Health Protection of Consumers and Veterinary Medicine, Jena, Germany.
authors.forename: H
authors.initials: H
authors.lastname: Köhler
authors.forename: B
authors.initials: B
authors.lastname: Lemser
authors.forename: G
authors.initials: G
authors.lastname: Müller
authors.forename: A
authors.initials: A
authors.lastname: Saalmüller
mesh_terms: D000336:Aerosols; D000818:Animals; D001992:Bronchoalveolar Lavage Fluid / Q000276:immunology*; D007114:Immunization; D016130:Immunophenotyping; D008214:Lymphocytes / Q000276:immunology*; D016979:Pasteurella multocida / Q000276:immunology*; D013552:Swine
chemical_list: D000336:Aerosols