title: Human acellular dermal matrix as a novel model of malignant epithelial cell invasion.
abstract: Cancer invasion and metastasis are associated with matrix degradation. We describe a novel in vivo model of invasion by squamous epithelial neoplastic cells derived from transgenic mice grown on acellular human dermis. Human dermis was subjected to multiple freeze-thaw cycles to render it acellular, maintaining the basement membrane of the former dermal-epidermal junction. Cells representing discrete stages of a multistep transgenic mouse model of epidermal carcinogenesis (neonatal transgenic keratinocytes, moderately/poorly differentiated squamous cell carcinoma, and lymph node metastasis) were seeded onto the basement membrane surface, grown in culture for 4 days, grafted in a subpannicular pocket of athymic mice, and harvested after 3 weeks. Histological analysis demonstrated that neonatal transgenic keratinocytes did not degrade the basement membrane or invade the underlying dermis. In contrast, malignant cells derived from both a moderately differentiated squamous carcinoma and a lymph node metastasis were highly invasive. Immunohistochemical analysis revealed collagenase only in nests of invading malignant cells in contact with the dermal matrix, but not in the tumor mass remaining above the basement membrane, suggesting that this proteinase may be required for stromal invasion. This novel model recapitulates the events seen in malignant invasion: transgenic keratinocytes are unable to penetrate the dermis while cells from a moderately differentiated carcinoma and from lymph node metastasis consistently invade.
authors.affiliation: Department of Surgery, University of California, San Francisco 94110, USA.
authors.forename: K M
authors.initials: KM
authors.lastname: Bullard
authors.forename: M J
authors.initials: MJ
authors.lastname: Banda
authors.forename: J M
authors.initials: JM
authors.lastname: Arbeit
authors.forename: E
authors.initials: E
authors.lastname: Bergsland
authors.forename: D M
authors.initials: DM
authors.lastname: Young
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D002294:Carcinoma, Squamous Cell / Q000378:metabolism / Q000473:pathology; D002454:Cell Differentiation; D017364:Collagenases / Q000378:metabolism; D004848:Epithelium / Q000473:pathology; D005109:Extracellular Matrix; D006801:Humans; D015603:Keratinocytes / Q000378:metabolism / Q000473:pathology*; D008198:Lymph Nodes / Q000473:pathology; D008207:Lymphatic Metastasis; D051379:Mice; D008822:Mice, Transgenic; D009361:Neoplasm Invasiveness; D027383:Papillomaviridae / Q000235:genetics; D012867:Skin / Q000166:cytology / Q000473:pathology*; D014407:Tumor Cells, Cultured
chemical_list: D017364:Collagenases