title: Survival of nigral grafts within the striatum of marmosets with 6-OHDA lesions depends critically on donor embryo age.
abstract: The study examined the importance of embryonic donor age for the survival of nigral grafts in 6-OHDA-lesioned marmosets. The issue as to whether donor age is critical for the survival of nigral grafts in primates is controversial, because several early reports suggested that relatively old tissue could survive transplantation and produce functional benefits in monkeys, in contrast to the restrictive time dependence observed in rodents. Embryonic marmoset donors embryos of three different ages were employed: 1) E74 (Carnegie stage 18-19); 2) E83-84 (Carnegie stage 23+); 3) E92-93 (foetal period). The nigral neurons derived from the ventral mesencephalon in the two older donor age groups did not survive well when grafted to the striatum of adult marmosets with unilateral 6-OHDA lesions. Although a few tyrosine hydroxylase (TH+) neurons could be identified by immunohistochemistry at graft sites in all recipients in older donor age groups, the numbers of surviving neurons in these were small, on average typically less than 100 TH+ cells. These small grafts were not sufficient to affect amphetamine-induced rotation. In contrast, many more TH+ cells typically survived transplantation in the recipients of graft tissue derived from the youngest donors and amphetamine-induced rotation was significantly reduced in this group alone. The time course and extent of the reduction in rotation was remarkably similar to that observed in previous marmoset nigral graft studies, confirming the utility of amphetamine-induced rotation as a sensitive and reliable indicator of nigral graft function in this species. Considering these results and other recent evidence from monkey to monkey, human to rat, and human to human graft studies, the survival of embryonic nigral tissues derived from primate donors transplanted into the striatum does appear to be critically dependent on the age of the donor tissue.
authors.affiliation: Department of Experimental Psychology, University of Cambridge, UK.
authors.forename: L E
authors.initials: LE
authors.lastname: Annett
authors.forename: E M
authors.initials: EM
authors.lastname: Torres
authors.forename: D J
authors.initials: DJ
authors.lastname: Clarke
authors.forename: Y
authors.initials: Y
authors.lastname: Ishida
authors.forename: R A
authors.initials: RA
authors.lastname: Barker
authors.forename: R M
authors.initials: RM
authors.lastname: Ridley
authors.forename: H F
authors.initials: HF
authors.lastname: Baker
authors.forename: S B
authors.initials: SB
authors.lastname: Dunnett
mesh_terms: D000818:Animals; D016380:Brain Tissue Transplantation; D002144:Callithrix; D002421:Caudate Nucleus; D017690:Cell Transplantation; D004195:Disease Models, Animal; D016332:Fetal Tissue Transplantation; D005865:Gestational Age; D006085:Graft Survival; D016627:Oxidopamine; D010302:Parkinson Disease, Secondary / Q000139:chemically induced / Q000601:surgery*; D013378:Substantia Nigra / Q000166:cytology / Q000196:embryology / Q000637:transplantation*
chemical_list: D016627:Oxidopamine