Use of transgenic mutational test systems in risk assessment of carcinogens.
Two transgenic in vivo mutation assays are described which are based on LacZ (Muta Mouse) and LacI (Big Blue) shuttle vector systems. Their utility has already been explored by a number of investigators including our laboratory. The evaluation of data derived from these assays confirm that they offer a practical method for studying mutagenic activity and mechanism in a wide range of tissues including those of the respiratory and gastrointestinal tract. Therefore, these transgenic mutation assays are valuable tools to assess the organotropic effects of genotoxic carcinogens.
Schmezer
P
P
Division of Toxicology and Cancer Risk Factors, German Cancer Research Centre, Heidelberg, Germany.
Eckert
C
C
Liegibel
U M
UM
Klein
R G
RG
Bartsch
H
H
D000818:Animals; D030801:Animals, Genetically Modified; D002273:Carcinogens / Q000633:toxicity; D051379:Mice; D008822:Mice, Transgenic; D009152:Mutagenicity Tests / Q000379:methods*; D009153:Mutagens / Q000633:toxicity; D009603:Nitroso Compounds / Q000633:toxicity; D051381:Rats; D018570:Risk Assessment
D002273:Carcinogens; D009153:Mutagens; D009603:Nitroso Compounds; C008651:4-nitrosodimethylaniline