Introns enable the polyomavirus middle and small T antigens to stimulate the growth of primary rat embryo fibroblasts.
We constructed spliceable vectors that separately encode polyomavirus MT and ST. The addition of an intron enables MT to transform and to immortalize more efficiently and ST to transiently stimulate the growth of primary rat embryo fibroblasts.
Asselin
C
C
Department of Anatomy and Cell Biology, University of Sherbrooke, Quebec, Canada.
Sullivan
M
M
Bastin
M
M
D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology; D000952:Antigens, Polyomavirus Transforming / Q000235:genetics* / Q000502:physiology*; D002455:Cell Division / Q000187:drug effects; D002470:Cell Survival / Q000235:genetics; D002472:Cell Transformation, Viral; D005347:Fibroblasts; D005786:Gene Expression Regulation; D005822:Genetic Vectors; D005839:Gentamicins / Q000494:pharmacology; D007438:Introns / Q000235:genetics*; D016297:Mutagenesis, Site-Directed; D016133:Polymerase Chain Reaction; D012326:RNA Splicing; D051381:Rats; D016196:S Phase / Q000235:genetics; D014158:Transcription, Genetic; D014162:Transfection
D000900:Anti-Bacterial Agents; D000952:Antigens, Polyomavirus Transforming; D005839:Gentamicins; C010680:antibiotic G 418