title: The Pseudomonas aeruginosa quorum-sensing signal molecule N-(3-oxododecanoyl)-L-homoserine lactone has immunomodulatory activity.
abstract: Diverse gram-negative bacterial cells communicate with each other by using diffusible N-acyl homoserine lactone (AHL) signal molecules to coordinate gene expression with cell population density. Accumulation of AHLs above a threshold concentration renders the population "quorate," and the appropriate target gene is activated. In pathogenic bacteria, such as Pseudomonas aeruginosa, AHL-mediated quorum sensing is involved in the regulation of multiple virulence determinants. We therefore sought to determine whether the immune system is capable of responding to these bacterial signal molecules. Consequently the immunomodulatory properties of the AHLs N-(3-oxododecanoyl)-L-homoserine lactone (OdDHL) and N-(3-oxohexanoyl)-L-homoserine lactone (OHHL) were evaluated in murine and human leukocyte immunoassays in vitro. OdDHL, but not OHHL, inhibited lymphocyte proliferation and tumor necrosis factor alpha production by lipopolysaccharide-stimulated macrophages. Furthermore, OdDHL simultaneously and potently down-regulated the production of IL-12, a Th-1-supportive cytokine. At high concentrations (>7 x 10(-5) M) OdDHL inhibited antibody production by keyhole limpet hemocyanin-stimulated spleen cells, but at lower concentrations (<7 x 10(-5) M), antibody production was stimulated, apparently by increasing the proportion of the immunoglobulin G1 (IgG1) isotype. OdDHL also promoted IgE production by interleukin-4-stimulated human peripheral blood mononuclear cells. These data indicate that OdDHL may influence the Th-1-Th-2 balance in the infected host and suggest that, in addition to regulating the expression of virulence determinants, OdDHL may contribute to the pathogenesis of P. aeruginosa infections by functioning as a virulence determinant per se.
authors.affiliation: Department of Life Science, University of Nottingham, University Park, United Kingdom.
authors.forename: G
authors.initials: G
authors.lastname: Telford
authors.forename: D
authors.initials: D
authors.lastname: Wheeler
authors.forename: P
authors.initials: P
authors.lastname: Williams
authors.forename: P T
authors.initials: PT
authors.lastname: Tomkins
authors.forename: P
authors.initials: P
authors.lastname: Appleby
authors.forename: H
authors.initials: H
authors.lastname: Sewell
authors.forename: G S
authors.initials: GS
authors.lastname: Stewart
authors.forename: B W
authors.initials: BW
authors.lastname: Bycroft
authors.forename: D I
authors.initials: DI
authors.lastname: Pritchard
mesh_terms: D015107:4-Butyrolactone / Q000031:analogs & derivatives* / Q000096:biosynthesis / Q000276:immunology / Q000302:isolation & purification; D000276:Adjuvants, Immunologic; D000595:Amino Acid Sequence; D000818:Animals; D002455:Cell Division; D002478:Cells, Cultured; D003208:Concanavalin A / Q000276:immunology; D003601:Cytotoxicity Tests, Immunologic; D005260:Female; D006433:Hemocyanins / Q000276:immunology; D006714:Homoserine / Q000031:analogs & derivatives* / Q000096:biosynthesis / Q000276:immunology / Q000302:isolation & purification; D006801:Humans; D007073:Immunoglobulin E / Q000096:biosynthesis; D007074:Immunoglobulin G / Q000096:biosynthesis; D018664:Interleukin-12 / Q000378:metabolism; D015847:Interleukin-4 / Q000276:immunology; D019169:Jurkat Cells; D007963:Leukocytes, Mononuclear / Q000378:metabolism; D008070:Lipopolysaccharides / Q000276:immunology; D008214:Lymphocytes / Q000166:cytology / Q000276:immunology* / Q000378:metabolism; D008264:Macrophages / Q000378:metabolism; D017737:Macrophages, Peritoneal / Q000276:immunology / Q000378:metabolism; D051379:Mice; D008807:Mice, Inbred BALB C; D008969:Molecular Sequence Data; D011505:Protein-Tyrosine Kinases / Q000378:metabolism; D011550:Pseudomonas aeruginosa / Q000276:immunology* / Q000472:pathogenicity; D015398:Signal Transduction / Q000276:immunology; D013154:Spleen / Q000166:cytology / Q000276:immunology; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism; D014774:Virulence / Q000276:immunology
chemical_list: D000276:Adjuvants, Immunologic; D007074:Immunoglobulin G; D008070:Lipopolysaccharides; C109860:N-(3-oxododecanoyl)homoserine lactone; D014409:Tumor Necrosis Factor-alpha; D003208:Concanavalin A; D018664:Interleukin-12; D015847:Interleukin-4; D007073:Immunoglobulin E; D006714:Homoserine; C030654:N-(3-oxohexanoyl)-3-aminodihydro-2(3H)-furanone; D006433:Hemocyanins; D011505:Protein-Tyrosine Kinases; D015107:4-Butyrolactone