title: The novel genes, cbbQ and cbbO, located downstream from the RubisCO genes of Pseudomonas hydrogenothermophila, affect the conformational states and activity of RubisCO.
abstract: The cbbQ and cbbO genes are located downstream from the RubisCO genes (cbbLS) in the thermophilic hydrogen-oxidizing bacterium, Pseudomonas hydrogenothermophila. Recombinant RubisCO enzymes were purified from E. coli cells which were transformed with plasmids expressing cbbLS, cbbLSQ, cbbLSO, or cbbLSQO. Co-expression of cbbQ and/or cbbO with cbbLS made the maximal rates of carboxylation (Vmax) of the recombinant RubisCOs about two-fold higher than that of the enzyme derived from only cbbLS. The RubisCOs with high Vmax also had a high stability when undergoing ultrasonic treatment. The results of the circular dichroism spectra and the 8-anilino-1-naphthalenesulfonate binding assay indicated that these recombinant RubisCOs were conformationally different to each other.
authors.affiliation: Department of Biotechnology, University of Tokyo, Japan.
authors.forename: N R
authors.initials: NR
authors.lastname: Hayashi
authors.forename: H
authors.initials: H
authors.lastname: Arai
authors.forename: T
authors.initials: T
authors.lastname: Kodama
authors.forename: Y
authors.initials: Y
authors.lastname: Igarashi
mesh_terms: D001426:Bacterial Proteins / Q000235:genetics; D002352:Carrier Proteins / Q000235:genetics*; D004789:Enzyme Activation; D004795:Enzyme Stability; D005798:Genes, Bacterial; D011487:Protein Conformation; D011499:Protein Processing, Post-Translational; D011549:Pseudomonas / Q000201:enzymology / Q000235:genetics*; D011994:Recombinant Proteins / Q000737:chemistry / Q000378:metabolism; D012273:Ribulose-Bisphosphate Carboxylase / Q000737:chemistry / Q000378:metabolism
chemical_list: D001426:Bacterial Proteins; D002352:Carrier Proteins; C092405:CbbQ protein, bacteria; D011994:Recombinant Proteins; D012273:Ribulose-Bisphosphate Carboxylase