Involvement of H2O2 production in cisplatin-induced nephrotoxicity.
The purpose of this study was to elucidate the involvement of H2O2 production in cisplatin-induced nephrotoxicity. The S3 cells were more sensitive than OMCT cells in cell viability and H2O2 production during cisplatin treatment. The cytotoxicity and H2O2 production induced by cisplatin were terminated by treatment with catalase. In conclusion, we emphasize the importance of the hydroxy radical in cisplatin nephrotoxicity.
Tsutsumishita
Y
Y
Faculty of Pharmaceutical Sciences, University of Tokushima, Japan.
Onda
T
T
Okada
K
K
Takeda
M
M
Endou
H
H
Futaki
S
S
Niwa
M
M
D000818:Animals; D002374:Catalase / Q000378:metabolism; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D002945:Cisplatin / Q000494:pharmacology / Q000633:toxicity*; D005452:Fluoresceins / Q000378:metabolism; D005456:Fluorescent Dyes / Q000378:metabolism; D006861:Hydrogen Peroxide / Q000032:analysis / Q000378:metabolism*; D017665:Hydroxyl Radical / Q000633:toxicity; D007684:Kidney Tubules / Q000187:drug effects*; D051379:Mice; D008822:Mice, Transgenic; D018613:Microscopy, Confocal
D005452:Fluoresceins; D005456:Fluorescent Dyes; C029569:diacetyldichlorofluorescein; D017665:Hydroxyl Radical; D006861:Hydrogen Peroxide; D002374:Catalase; D002945:Cisplatin