Sexual and asexual development of Cryptosporidium parvum in five oocyst- or sporozoite-infected human enterocytic cell lines.
The human enterocytic cell lines Caco-2, HT29, HCT8 and the Caco-2 clones TC7 and PF11 were studied for their ability to support Cryptosporidium parvum development. Following the addition in cultures of either oocysts or excysted sporozoites, immunofluorescent and transmission electron microscopy revealed the presence of all stages of the parasite life cycle by both procedures, and no difference in the ratio of infected cells was found among cell lines. More oocysts were seen in cell monolayers infected with oocysts than with sporozoites (p < 0.0001). The number of meronts observed was the same after either oocysts or sporozoites inoculation. Data suggest that the two methods yield a same cell infection rate.
Maillot
C
C
Laboratoire de Parasitologie, Hôpital Charles Nicolle, Rouen, France.
Favennec
L
L
Francois
A
A
Ducrotte
P
P
Brasseur
P
P
D000818:Animals; D018938:Caco-2 Cells; D002417:Cattle; D016785:Cryptosporidium parvum / Q000254:growth & development*; D005243:Feces / Q000469:parasitology; D006801:Humans; D007420:Intestine, Large / Q000469:parasitology*; D014407:Tumor Cells, Cultured