Genomic DNA from mice: a comparison of recovery methods and tissue sources.
Our aim is to identify an extraction method and the source of mouse tissue(s) that could allow a high-resolution genomic scan from a living mouse. We compared and optimized two methods for yield, purity of DNA, and their use in the polymerase chain reaction (PCR) of DNA extracted from different mouse tissues. In addition to whole blood, tissue samples from the brain, liver, testis, and tail were included in this study. The Rapid Method (RM) is preferable for the whole blood samples and testis and brain tissue samples because it is quicker, less toxic, and more cost-effective than the proteinase K method (PM). For liver the PM produced higher yields of DNA with less degradation than the RM. For tail tip, the PM produced a higher yield of DNA, but the RM resulted in a higher yield of PCR product. From a living mouse, a tail snip generated a sufficient amount of DNA for several hundred PCRs but not a complete genomic scan. We suggest that the RM can be used to extract genomic DNA for a complete genomic scan which requires either testicular tissues or repeated blood samples from the suborbital sinus over several months without sacrificing the animal.
Hofstetter
J R
JR
Department of Psychiatry, Indiana University School of Medicine, Indianapolis 46202, USA.
Zhang
A
A
Mayeda
A R
AR
Guscar
T
T
Nurnberger
J I
JI
Lahiri
D K
DK
D000818:Animals; D001923:Brain Chemistry / Q000235:genetics; D004247:DNA / Q000097:blood / Q000302:isolation & purification*; D016678:Genome; D008099:Liver / Q000737:chemistry; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D009928:Organ Specificity / Q000235:genetics*; D016133:Polymerase Chain Reaction; D013623:Tail / Q000737:chemistry; D013737:Testis / Q000737:chemistry
D004247:DNA