Metabotropic glutamate receptor in GHRH and beta-endorphin neurones of the hypothalamic arcuate nucleus.
Growth hormone-releasing hormone (GHRH) and beta-endorphin are mainly synthesized in neurones of the hypothalamic arcuate nucleus. Arcuate neurones also contain both ionotropic and metabotropic glutamate receptors. The aim of present study was to investigate whether glutamate receptors are present in GHRH and beta-endorphin containing nerve cells of this hypothalamic area. Using double-label immunocytochemistry as well as the mirror technique, we found that almost all GHRH and beta-endorphin immunoreactive arcuate neurones contain the metabotropic glutamate receptor la. The observations provide morphological evidence for the view that glutamate, which appears to be a major excitatory neurotransmitter in the hypothalamus, may directly stimulate GHRH and beta-endorphin neurones of the medial hypothalamus.
Kiss
J
J
Department of Human Morphology and Developmental Biology, Semmelweis University of Medicine, Budapest, Hungary.
Kocsis
K
K
Csáki
A
A
Görcs
T J
TJ
Halász
B
B
D000818:Animals; D000911:Antibodies, Monoclonal; D001111:Arcuate Nucleus of Hypothalamus / Q000166:cytology* / Q000502:physiology; D013007:Growth Hormone-Releasing Hormone / Q000032:analysis*; D007031:Hypothalamus / Q000502:physiology; D007150:Immunohistochemistry; D008297:Male; D009474:Neurons / Q000166:cytology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D018094:Receptors, Metabotropic Glutamate / Q000032:analysis*; D001615:beta-Endorphin / Q000032:analysis*
D000911:Antibodies, Monoclonal; D018094:Receptors, Metabotropic Glutamate; C104077:metabotropic glutamate receptor type 1; D001615:beta-Endorphin; D013007:Growth Hormone-Releasing Hormone