Non-mutagenic repair of (6-4)photoproducts by (6-4)photolyase purified from Drosophila melanogaster.
The (6-4)photoproduct DNA photolyase ((6-4)photolyase) repairs UV-induced pyrimidine (6-4) pyrimidone photoproduct ((6-4)photoproduct, pyr[6,4]pyr) in a light dependent manner. Drosophila (6-4)photolyase was purified to near homogeneity from Drosophila embryonic cells and is shown to be a 62 kDa protein as determined by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. The purified (6-4)photolyase repairs (6-4)photoproducts induced at 5'-CC-3' site (C[6,4]C) as well as T[6,4]T and T[6,4]C. Photoreactivation of (6-4)photoproduct constructed in M13 phage eliminates the replication block and abolishes induced mutagenesis in E. coli cells, suggesting that the (6-4)photolyase repairs the photoproduct to the unmodified form.
Todo
T
T
Department of Radiation Biology, Faculty of Medicine, Osaka Univ., Japan. todo@house.rbc.kyoto-u.ac.jp
Ryo
H
H
Borden
A
A
Lawrence
C
C
Sakaguchi
K
K
Hirata
H
H
Nomura
T
T
D000818:Animals; D017104:Bacteriophage M13; D001483:Base Sequence; D018736:DNA Adducts; D004256:DNA Polymerase I / Q000378:metabolism; D004260:DNA Repair / Q000502:physiology*; D004261:DNA Replication; D004279:DNA, Viral; D004255:Deoxyribodipyrimidine Photo-Lyase / Q000737:chemistry / Q000302:isolation & purification* / Q000378:metabolism*; D004331:Drosophila melanogaster / Q000201:enzymology*; D008969:Molecular Sequence Data; D008970:Molecular Weight; D011740:Pyrimidine Dimers / Q000378:metabolism*; D014466:Ultraviolet Rays
D018736:DNA Adducts; D004279:DNA, Viral; D011740:Pyrimidine Dimers; D004256:DNA Polymerase I; D004255:Deoxyribodipyrimidine Photo-Lyase