title: Electron microscopic observation of delta-opioid receptor-1 in the rat area postrema.
abstract: The ultrastructural localization of delta-1-opioid-receptor in the rat area postrema was quantitatively studied by pre-embedding avidin-biotin-peroxidase-complex technique. Most of the immunoreactive profiles (67.4%) observed in the present study were axon terminals, whereas the immunopositive dendrites were less (28.3%). Within the axon terminals, the immunoreactivity was found stronger in the dense-cored vesicles than in the small, clear, and round vesicles. Almost 2/3 of the DOR-1 immunoreactive axon terminals had DAB reacted dense-cored vesicles. About half of the immunopositive axon terminals were found to make synapse to dendrites. The dendrites postsynaptic to DOR-1 immunoreactive axon terminals were identified as DOR-1 immunoreactive or not, mainly according to the immunoreactive appearance of the postsynaptic membrane. About half of the DOR-1 immunoreactive dendrites were observed to receive synapse: most of them have their immunoreactivity results at the postsynaptic membranes.
authors.affiliation: Department of Anatomy, Showa University School of Medicine, Tokyo, Japan.
authors.forename: J L
authors.initials: JL
authors.lastname: Guan
authors.forename: Q P
authors.initials: QP
authors.lastname: Wang
authors.forename: Y
authors.initials: Y
authors.lastname: Nakai
mesh_terms: D000818:Animals; D001360:Avidin / Q000031:analogs & derivatives; D001369:Axons / Q000737:chemistry; D001710:Biotin; D002552:Cerebral Ventricles / Q000737:chemistry* / Q000648:ultrastructure; D003712:Dendrites / Q000737:chemistry / Q000648:ultrastructure; D006735:Horseradish Peroxidase; D007124:Immunoenzyme Techniques; D008297:Male; D016253:Microscopy, Immunoelectron; D051381:Rats; D017208:Rats, Wistar; D017465:Receptors, Opioid, delta / Q000032:analysis*
chemical_list: D017465:Receptors, Opioid, delta; C083986:avidin-horseradish peroxidase complex; D001360:Avidin; D001710:Biotin; D006735:Horseradish Peroxidase