title: Effects of glutamine supplementation on circulating lymphocytes after bone marrow transplantation: a pilot study.
abstract: Glutamine (Gln) is an important nutrient substrate for lymphocytes and macrophages in vitro. This pilot study evaluated effects of Gln supplementation on circulating lymphocytes and lymphocyte subsets after allogeneic bone marrow transplantation (BMT). Adult patients received either parenteral nutrition supplemented by L-Gln (Gln-PN) or standard Gln-free PN after BMT. Leukocyte and total lymphocyte counts were determined during hospitalization, and flow cytometry studies of peripheral mononuclear cells were performed 1 to 2 weeks after hospital discharge. The Gln-PN group demonstrated a higher percentage of blood lymphocytes during hospitalization. At flow cytometry, patients who received Gln-PN had an increased total lymphocyte count (332 +/- 50 versus 590 +/- 71 cells/microL, P = 0.010); greater numbers of total T lymphocytes (54 +/- 19 versus 229 +/- 70 cells/microL, P = 0.030); and higher CD4+ and CD8+ T-lymphocyte counts in peripheral blood compared with controls. Gln-PN may support lymphocyte recovery after BMT.
authors.affiliation: Department of Medicine, Emory University School of Medicine, Atlanta, Georgia, USA. tzieg01@emory.edu
authors.forename: T R
authors.initials: TR
authors.lastname: Ziegler
authors.forename: R L
authors.initials: RL
authors.lastname: Bye
authors.forename: R L
authors.initials: RL
authors.lastname: Persinger
authors.forename: L S
authors.initials: LS
authors.lastname: Young
authors.forename: J H
authors.initials: JH
authors.lastname: Antin
authors.forename: D W
authors.initials: DW
authors.lastname: Wilmore
mesh_terms: D000328:Adult; D016026:Bone Marrow Transplantation / Q000276:immunology* / Q000502:physiology; D004311:Double-Blind Method; D005260:Female; D005434:Flow Cytometry; D005973:Glutamine / Q000627:therapeutic use*; D006086:Graft vs Host Disease / Q000453:epidemiology / Q000517:prevention & control; D006689:Hodgkin Disease / Q000276:immunology / Q000628:therapy; D006801:Humans; D007951:Leukemia, Myeloid / Q000276:immunology / Q000628:therapy; D007958:Leukocyte Count / Q000187:drug effects; D018655:Lymphocyte Count / Q000187:drug effects; D008214:Lymphocytes / Q000187:drug effects / Q000502:physiology*; D008297:Male; D010288:Parenteral Nutrition; D010865:Pilot Projects
chemical_list: D005973:Glutamine