mRNA encoding the catalytic subunit of DNA-dependent protein kinase is widely expressed in Xenopus cells.
Here, the sequence of a Xenopus laevis cDNA encoding the 640 carboxy-terminal amino acids of the catalytic subunit of DNA-dependent protein kinase (DNA-PKcs) is reported. The predicted Xenopus protein segment is 65% identical to the human counterpart. Northern blot analysis indicates that Xenopus DNA-PKcs is encoded by an approx. 13000 nt transcript. DNA-PKcs mRNA is widely expressed in adult tissues as well as in oocytes and embryos. It is also shown that outside the conserved kinase domain, Xenopus DNA-PKcs bears significant similarities to hypothetical 420.8 and 433.2 kDa proteins in yeast species.
Labhart
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The Scripps Research Institute, Department of Molecular and Experimental Medicine, La Jolla, CA 92037, USA. plabhart@scripps.edu
D000595:Amino Acid Sequence; D000818:Animals; D015152:Blotting, Northern; D002478:Cells, Cultured; D003001:Cloning, Molecular; D017931:DNA Primers; D051747:DNA-Activated Protein Kinase; D004268:DNA-Binding Proteins; D004625:Embryo, Nonmammalian / Q000201:enzymology; D019143:Evolution, Molecular; D018507:Gene Expression Regulation, Developmental; D006801:Humans; D008969:Molecular Sequence Data; D009687:Nuclear Proteins; D009865:Oocytes / Q000201:enzymology; D009866:Oogenesis; D017346:Protein Serine-Threonine Kinases / Q000032:analysis / Q000737:chemistry / Q000235:genetics*; D012333:RNA, Messenger / Q000032:analysis / Q000235:genetics*; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D014982:Xenopus laevis
D017931:DNA Primers; D004268:DNA-Binding Proteins; D009687:Nuclear Proteins; D012333:RNA, Messenger; D051747:DNA-Activated Protein Kinase; C497263:PRKDC protein, human; D017346:Protein Serine-Threonine Kinases