Possible role of nitric oxide in IgE-mediated allergic cutaneous reaction in mice.
The role of nitric oxide (NO) in the development of allergic cutaneous reactions in mice was investigated. A biphasic cutaneous reaction composed of an immediate-phase edema and a late-phase edema was evoked by epicutaneous application of 2,4-dinitrofluorobenzene on the ear of mice passively sensitized with mouse anti-dinitrophenol monoclonal IgE. Two NO synthase inhibitors, L-NG-nitroarginine methyl ester (L-NAME) and NG-monomethyl-L-arginine, significantly inhibited both phases of the IgE-mediated biphasic cutaneous reaction. Simultaneous treatment with L-arginine attenuated the inhibitory effect of L-NAME. An NO donor, 3-morpholinosydononimine-N-ethylcarbamide, caused a potent edematous reaction in the mouse ear. Furthermore, L-NAME inhibited the cutaneous reactions caused by both interleukin-1beta and tumor necrosis factor-alpha, putative mediators of the late-phase edema in the biphasic cutaneous reaction. These results indicate that a gaseous mediator, NO, participates, at least in part, in the development of ear edema in the IgE-mediated biphasic cutaneous reaction in mice.
Musoh
K
K
Department of Pharmacology, Gifu Pharmaceutical University, Japan.
Nakamura
N
N
Ueda
Y
Y
Inagaki
N
N
Nagai
H
H
D000818:Animals; D001120:Arginine / Q000494:pharmacology; D004139:Dinitrofluorobenzene / Q000276:immunology; D004140:Dinitrophenols / Q000276:immunology; D004487:Edema / Q000276:immunology*; D005260:Female; D007116:Immunization, Passive; D007073:Immunoglobulin E / Q000276:immunology*; D007375:Interleukin-1 / Q000276:immunology / Q000494:pharmacology; D051379:Mice; D008807:Mice, Inbred BALB C; D008981:Molsidomine / Q000031:analogs & derivatives / Q000494:pharmacology; D019331:NG-Nitroarginine Methyl Ester / Q000494:pharmacology; D009569:Nitric Oxide / Q000378:metabolism* / Q000502:physiology*; D014409:Tumor Necrosis Factor-alpha / Q000276:immunology / Q000494:pharmacology; D018366:Vasculitis, Leukocytoclastic, Cutaneous / Q000276:immunology* / Q000378:metabolism*; D019323:omega-N-Methylarginine / Q000494:pharmacology
D004140:Dinitrophenols; D007375:Interleukin-1; D014409:Tumor Necrosis Factor-alpha; D019323:omega-N-Methylarginine; D009569:Nitric Oxide; D007073:Immunoglobulin E; C002385:linsidomine; D001120:Arginine; D004139:Dinitrofluorobenzene; D008981:Molsidomine; D019331:NG-Nitroarginine Methyl Ester