title: Confirmation of anatoxin-a(s), in the cyanobacterium Anabaena lemmermannii, as the cause of bird kills in Danish lakes.
abstract: Cyanobacterial blooms were implicated in bird kills at lakes in Denmark in July 1993 and June-July 1994. These blooms were dominated by Anabaena lemmermannii and were shown to contain a neurotoxin with anticholinesterase activity. In this study, the toxin was isolated by mouse lethality guided column chromatographies from the field sample collected at Lake Knud sø in 1993. Various spectroscopic data indicated that the toxin was anatoxin-a(s), an irreversible anticholinesterase, first reported in Anabaena flos-aquae. Chemical detection of the same toxin in cultured A. lemmermannii also confirmed this species as the cause of the deaths of the wild birds.
authors.affiliation: Department of Applied Biological Chemistry, Faculty of Agriculture, Tohoku University, Sendai, Japan.
authors.forename: H
authors.initials: H
authors.lastname: Onodera
authors.forename: Y
authors.initials: Y
authors.lastname: Oshima
authors.forename: P
authors.initials: P
authors.lastname: Henriksen
authors.forename: T
authors.initials: T
authors.lastname: Yasumoto
mesh_terms: D017033:Anabaena / Q000737:chemistry*; D000818:Animals; D001427:Bacterial Toxins / Q000032:analysis* / Q000633:toxicity; D001717:Birds / Q000378:metabolism; D000087522:Cyanobacteria Toxins; D003718:Denmark; D008297:Male; D008387:Marine Toxins / Q000032:analysis* / Q000633:toxicity; D051379:Mice; D052998:Microcystins; D009498:Neurotoxins / Q000032:analysis* / Q000633:toxicity; D014326:Tropanes
chemical_list: D001427:Bacterial Toxins; D000087522:Cyanobacteria Toxins; D008387:Marine Toxins; D052998:Microcystins; D009498:Neurotoxins; D014326:Tropanes; C509783:anatoxin a