title: Epstein-Barr virus infection in non-carcinomatous gastric epithelium.
abstract: Gastric tissue specimens from 20 patients with chronic atrophic gastritis, one of whom also had an early gastric carcinoma, were studied for evidence of Epstein-Barr virus (EBV) infection by Southern blot analysis, DNA and RNA in situ hybridization, and immunohistochemistry for the presence of the EBV-determined nuclear antigen 1 (EBNA-1) and the latent membrane protein 1 (LMP-1). EBV DNA was detected in two cases with chronic atrophic gastritis and in the case with early gastric carcinoma by Southern blot hybridization. DNA in situ hybridization showed EBV genomes in the epithelial cells of two other cases with chronic atrophic gastritis and in non-carcinomatous and carcinomatous epithelial cells of the early gastric carcinoma case. EBNA-1 was detected in all cases. LMP-1 was detected in areas of intestinal metaplasia in eight patients with chronic atrophic gastritis. EBV-encoded small RNA 1 (EBER-1) expression was limited to carcinoma cells. These results show that gastric epithelium is frequently infected with EBV and suggest that prolonged EBV persistence may contribute to the development of gastric carcinoma.
authors.affiliation: First Department of Internal Medicine, Yamaguchi University School of Medicine, Japan.
authors.forename: H
authors.initials: H
authors.lastname: Yanai
authors.forename: K
authors.initials: K
authors.lastname: Takada
authors.forename: N
authors.initials: N
authors.lastname: Shimizu
authors.forename: Y
authors.initials: Y
authors.lastname: Mizugaki
authors.forename: M
authors.initials: M
authors.lastname: Tada
authors.forename: K
authors.initials: K
authors.lastname: Okita
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000956:Antigens, Viral / Q000032:analysis; D015139:Blotting, Southern; D004279:DNA, Viral / Q000032:analysis; D019309:Epstein-Barr Virus Nuclear Antigens / Q000032:analysis; D005260:Female; D005753:Gastric Mucosa / Q000821:virology; D005757:Gastritis, Atrophic / Q000382:microbiology / Q000473:pathology / Q000821:virology*; D016480:Helicobacter pylori / Q000302:isolation & purification; D006566:Herpesviridae Infections / Q000209:etiology* / Q000473:pathology; D004854:Herpesvirus 4, Human / Q000235:genetics / Q000276:immunology; D006801:Humans; D007124:Immunoenzyme Techniques; D017403:In Situ Hybridization; D008297:Male; D008679:Metaplasia / Q000821:virology; D008875:Middle Aged; D011230:Precancerous Conditions / Q000821:virology; D012367:RNA, Viral / Q000032:analysis; D013274:Stomach Neoplasms / Q000473:pathology / Q000821:virology*; D014412:Tumor Virus Infections / Q000209:etiology* / Q000473:pathology; D014763:Viral Matrix Proteins / Q000032:analysis
chemical_list: D000956:Antigens, Viral; D004279:DNA, Viral; C037662:EBV-associated membrane antigen, Epstein-Barr virus; D019309:Epstein-Barr Virus Nuclear Antigens; D012367:RNA, Viral; D014763:Viral Matrix Proteins