title: Cell-mediated immunosuppression in mice by street rabies virus not restored by calcium ionophore or PMA.
abstract: It is known that rabies virus can suppress the host immune system. In this study we demonstrate a depression of cell-mediated immunity in mice, peripherally infected with Thai street rabies virus. The cell-mediated cytolysis of spleen cells from mice increased transiently on day 5 after infection and declined rapidly thereafter until death. The proliferation of spleen cells stimulated with a T-cell mitogen such as phytohemagglutinin or concanavalin A, was significantly suppressed during the course of infection. There was also a marked suppression of IL-2 secretion in parallel with a decrease of the T-cell proliferative response to mitogen. The suppression of T-cell proliferation was not restored by treatment with a calcium ionophore (A 23187) or phorbol 12-myristate-13 acetate (PMA).
authors.affiliation: Queen Saovabha Memorial Institute (WHO Collaborating Center for Research on Rabies Pathogenesis), Thai Red Cross Society, Bangkok.
authors.forename: S
authors.initials: S
authors.lastname: Kasempimolporn
authors.forename: W
authors.initials: W
authors.lastname: Saengseesom
authors.forename: C
authors.initials: C
authors.lastname: Mitmoonpitak
authors.forename: S
authors.initials: S
authors.lastname: Akesowan
authors.forename: V
authors.initials: V
authors.lastname: Sitprija
mesh_terms: D000818:Animals; D000001:Calcimycin / Q000494:pharmacology*; D002118:Calcium / Q000378:metabolism; D002413:Cations, Divalent; D003600:Cytosol / Q000378:metabolism; D003602:Cytotoxicity, Immunologic / Q000187:drug effects; D004285:Dogs; D007111:Immunity, Cellular / Q000187:drug effects; D007165:Immunosuppression Therapy; D007376:Interleukin-2 / Q000096:biosynthesis; D007739:L Cells; D008213:Lymphocyte Activation / Q000187:drug effects; D051379:Mice; D008807:Mice, Inbred BALB C; D008934:Mitogens; D011493:Protein Kinase C / Q000037:antagonists & inhibitors; D011818:Rabies / Q000276:immunology* / Q000821:virology; D011820:Rabies virus / Q000276:immunology*; D013601:T-Lymphocytes / Q000201:enzymology / Q000276:immunology / Q000378:metabolism; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology*
chemical_list: D002413:Cations, Divalent; D007376:Interleukin-2; D008934:Mitogens; D000001:Calcimycin; D011493:Protein Kinase C; D013755:Tetradecanoylphorbol Acetate; D002118:Calcium