title: Cloning of a novel Y-box homology protein (chkYB-1HP) cDNA lacking the cold-shock domain.
abstract: We have isolated the complete cDNA clone of a novel 262 amino acid Chicken YB-1 Homology Protein (chkYB-1HP) by screening a chicken embryo cDNA expression library. While the chkYB-1HP is identical over its carboxyl-terminal 78 amino acids with the Y-box protein YB-1, it differs strikingly from all other Y-box transcription factors by lacking the cold-shock domain (CSD). We propose that proteins like chkYB-1HP that lack the CSD, but retain the hydrophilic carboxyl domain could regulate Y-box proteins through the formation of heterodimeric complexes.
authors.affiliation: Molecular Microbiology and Immunology, School of Medicine, University of Missouri, Columbia 65212, USA.
authors.forename: A
authors.initials: A
authors.lastname: Nambiar
authors.forename: J C
authors.initials: JC
authors.lastname: Kandala
authors.forename: J
authors.initials: J
authors.lastname: Svoboda
authors.forename: R V
authors.initials: RV
authors.lastname: Guntaka
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002478:Cells, Cultured; D002642:Chick Embryo; D003001:Cloning, Molecular; D003080:Cold Temperature; D018076:DNA, Complementary / Q000235:genetics*; D004268:DNA-Binding Proteins / Q000235:genetics*; D005347:Fibroblasts; D005786:Gene Expression Regulation / Q000235:genetics*; D008969:Molecular Sequence Data; D012333:RNA, Messenger / Q000032:analysis; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D013045:Species Specificity; D014157:Transcription Factors / Q000235:genetics*
chemical_list: D018076:DNA, Complementary; D004268:DNA-Binding Proteins; D012333:RNA, Messenger; D014157:Transcription Factors; C486170:Y-box homology protein, Gallus gallus