The role of microvascular permeability in the mechanism for stunned myocardium in rats.
The microvascular permeability of stunned myocardium in rats in vivo was studied with FITC-labeled albumin (FITC-BSA). It was found that 15 and 20 min of myocardial ischemia followed by 1 hr of reperfusion resulted in myocardial stunning. The concentrations of FITC-BSA in myocardial tissue were 240.6 +/- 7.8 (IS15) and 267.4 +/- 7.9 (IS20) micrograms/g myocardium in ischemic groups, respectively, which were significantly higher than those in the control group (166.0 +/- 7.9 micrograms/g myocardium; P < 0.01). In stunned groups, the concentrations were 224.8 +/- 11.8 (MS15) and 241.7 +/- 6.0 (MS20) micrograms/g myocardium, decreased from those in ischemic groups but still higher than those in control group by 35.4 and 45.6%, respectively. The more significant the concentration of FITC-BSA, the more serious the myocardial stunning. Electron microscopy revealed no significant vascular injury. The results suggest that the increase in microvascular permeability resulting from transient ischemia is functional and is involved in the pathogenesis of stunned myocardium.
Chen
J M
JM
Department of Cardiology, Changzheng Hospital, Second Military Medical University, Shanghai, People's Republic of China.
Gong
X Q
XQ
Zhong
J G
JG
Chen
S C
SC
Zhang
G Y
GY
Wu
Z G
ZG
Yang
Y J
YJ
D000818:Animals; D002199:Capillary Permeability; D004730:Endothelium, Vascular / Q000648:ultrastructure; D016650:Fluorescein-5-isothiocyanate / Q000008:administration & dosage / Q000031:analogs & derivatives; D008297:Male; D009200:Myocardial Contraction; D015428:Myocardial Reperfusion Injury / Q000473:pathology / Q000503:physiopathology; D017682:Myocardial Stunning / Q000473:pathology / Q000503:physiopathology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D012710:Serum Albumin, Bovine / Q000008:administration & dosage; D013050:Spectrometry, Fluorescence
C039217:fluorescein isothiocyanate bovine serum albumin; D012710:Serum Albumin, Bovine; D016650:Fluorescein-5-isothiocyanate