Interaction of P1 RepA with replication origin of plasmid Rts1: capability of an initiator protein inducing replication from a foreign origin.
Rts1 RepA and P1 RepA are trans-acting proteins essential for the initiation of replication of plasmid Rts1 and prophage P1, respectively. In this study, we found that, in vitro, P1 RepA bound to the Rts1 ori fragment and Rts1 incI fragment as strongly as Rts1 RepA. In addition P1 RepA, in trans, activated the Rts1 replication origin that was cloned in pBR322, thus allowing the ori plasmid to be maintained in a polA E. coli host. Under these conditions, however, the ori plasmid was unstable as compared with that when activated by Rts1 RepA. In addition, we found that Rts1 RepA showed no interaction with the P1 replication origin.
Li
Y F
YF
Department of Bacteriology, Shinshu University School of Medicine, Matsumoto, Japan.
Tabuchi
A
A
Terawaki
Y
Y
D017101:Bacteriophage P1 / Q000235:genetics / Q000378:metabolism*; D004265:DNA Helicases; D004261:DNA Replication; D004268:DNA-Binding Proteins; D004926:Escherichia coli / Q000378:metabolism / Q000821:virology; D010957:Plasmids / Q000235:genetics / Q000378:metabolism*; D011485:Protein Binding; D011506:Proteins / Q000378:metabolism*; D011533:Proviruses; D018741:Replication Origin; D015534:Trans-Activators; D014764:Viral Proteins / Q000378:metabolism
D004268:DNA-Binding Proteins; D011506:Proteins; D015534:Trans-Activators; D014764:Viral Proteins; C045264:replication initiator protein; D004265:DNA Helicases