The interaction of human LDL with the tegument of adult Schistosoma mansoni.
The occurrence of a receptor for human LDL was investigated in the tegument of adult Schistosoma mansoni employing several approaches. Binding of LDL to SDS-PAGE fractionated tegument proteins was measured directly on nitro-cellulose membranes and visualised by an anti-human LDL antibody. Proteins with an Mr of 60, 35 and 14 kDa were evidenced. Affinity chromatography of 125I-labelled tegument proteins on a LDL-Sepharose column, revealed the same pattern of proteins observed in the immunoblot experiments. Finally, the binding of human LDL to the intact tegument was measured by microcalorimetry. Binding was shown to be an exothermic reaction, releasing approximately 2500 kcal/mol, it was saturable, and reproducibly displayed a biphasic curve suggesting that binding of LDL to S. mansoni might occur through a two step process, initiated by a nonspecific hydrophobic interaction followed by a specific high affinity ligand-receptor reaction. Pre-treatment of the tegument with trypsin reduced the binding of LDL to the tegument. Furthermore, albumin, which is an abundant lipid carrier protein in the serum and thus a potential ligand, failed to release any measurable heat when incubated with the tegument.
Tempone
A J
AJ
Departamento de Bioquímica Médica ICB/CCS, Universidade Federal do Rio de Janeiro, Ilha do Fundão, RJ, Brazil.
Bianconi
M L
ML
Rumjanek
F D
FD
D000818:Animals; D002151:Calorimetry; D006224:Cricetinae; D015801:Helminth Proteins / Q000378:metabolism*; D006790:Host-Parasite Interactions; D006801:Humans; D008024:Ligands; D008077:Lipoproteins, LDL / Q000378:metabolism*; D011485:Protein Binding; D011973:Receptors, LDL / Q000378:metabolism; D012550:Schistosoma mansoni / Q000254:growth & development* / Q000378:metabolism*
D015801:Helminth Proteins; D008024:Ligands; D008077:Lipoproteins, LDL; D011973:Receptors, LDL