[The importance of studies of the glycosaminoglycans in the peripheral blood leukocytes of patients in the diagnosis of the phases of chronic myeloleukemia].
To elucidate new diagnostic markers of chronic myeloid leukemia (CML) phases, we investigated quantitative and qualitative composition of glycosaminoglycans (GAG) of leukocytes from peripheral blood of 72 patients. Chronic CML phase was characterized by elevated GAG levels (2 times compared to normal values), weakening of anionic properties of chondroitin sulfate (CS) and high amount of heparan sulfate (HS). In CML transformation in the progressive phase overall concentration of GAG grew still higher, GAG fraction composition changed. In the blast crisis there was a sharp fall in the overall GAG, new electrophoretic fractions emerged. In the myeloid variant of the crisis an additional GAG component appeared (GAG-m), whereas in the lymphoid variant another component was found (GAG-1). It is suggested that the number and composition of GAG in peripheral blood leukocytes may serve markers of CML phase.
Kharchenko
M F
MF
Bessmel'tsev
S S
SS
Rukavitsyn
O A
OA
Abdulkadyrov
K M
KM
Bitiukova
E S
ES
Blinov
M N
MN
D015415:Biomarkers / Q000097:blood; D001752:Blast Crisis / Q000097:blood / Q000175:diagnosis; D002469:Cell Separation; D018450:Disease Progression; D004588:Electrophoresis, Cellulose Acetate; D006025:Glycosaminoglycans / Q000097:blood*; D006801:Humans; D015464:Leukemia, Myelogenous, Chronic, BCR-ABL Positive / Q000097:blood / Q000175:diagnosis* / Q000473:pathology; D007962:Leukocytes / Q000737:chemistry*
D015415:Biomarkers; D006025:Glycosaminoglycans