The microtubular system and posttranslationally modified tubulin during spermatogenesis in a parasitic nematode with amoeboid and aflagellate spermatozoa.
Using transmission electron microscopy and immunologic approaches with various antibodies against general tubulin and posttranslationally modified tubulin, we investigated microtubule organization during spermatogenesis in Heligmosomoides polygyrus, a species in which a conspicuous but transient microtubular system exists in several forms: a cytoplasmic network in the spermatocyte, the meiotic spindle, a perinuclear network and a longitudinal bundle of microtubules in the spermatid. This pattern differs from most nematodes including Caenorhabditis elegans, in which spermatids have not microtubules. In the spermatozoon of H. polygyrus, immunocytochemistry does not detect tubulin, but electron microscopy reveals two centrioles with a unique structure of 10 singlets. In male germ cells, microtubules are probably involved in cell shaping and positioning of organelles but not in cell motility. In all transient tubulin structures described in spermatocytes and spermatids of H. polygyrus, detyrosination, tyrosination, and polyglutamylation were detected, but acetylation and polyglycylation were not. The presence/absence of these posttranslational modifications is apparently not stage dependent. This is the first study of posttranslationally modified tubulin in nematode spermatogenesis.
Mansir
A
A
Laboratoire de Biologie parasitaire, Muséum national d'histoire naturelle, Paris, France.
Justine
J L
JL
D000818:Animals; D000909:Antibodies, Helminth / Q000032:analysis; D002463:Cell Membrane Permeability; D005404:Fixatives; D005407:Flagella / Q000502:physiology; D015801:Helminth Proteins / Q000378:metabolism; D007150:Immunohistochemistry; D008297:Male; D051379:Mice; D008870:Microtubules / Q000276:immunology / Q000502:physiology* / Q000648:ultrastructure; D009351:Nematospiroides dubius; D011499:Protein Processing, Post-Translational / Q000502:physiology*; D013087:Spermatids / Q000648:ultrastructure; D013091:Spermatogenesis / Q000276:immunology; D013094:Spermatozoa / Q000166:cytology* / Q000378:metabolism / Q000648:ultrastructure; D014404:Tubulin / Q000276:immunology / Q000378:metabolism*
D000909:Antibodies, Helminth; D005404:Fixatives; D015801:Helminth Proteins; D014404:Tubulin