Differentiation of Campylobacter isolates on the basis of sensitivity to boiling in water as measured by PCR-detectable DNA.
Differential sensitivity for the release of PCR-detectable genomic DNA upon boiling in water is reported for 45 Campylobacter jejuni and Campylobacter coli strains isolated in Egypt. All of the strains released PCR-detectable DNA when treated with proteinase K and sodium dodecyl sulfate. When DNA was extracted from these strains by boiling in water, nine (20%) of the strains were PCR negative or resistant to boiling, suggesting the presence of boiling-sensitive and boiling-resistant phenotypes.
Mohran
Z S
ZS
U.S. Naval Medical Research Unit No. 3, Cairo, Egypt. namru3_RSD@centcom.dsaa.osd.mil
Arthur
R R
RR
Oyofo
B A
BA
Peruski
L F
LF
Wasfy
M O
MO
Ismail
T F
TF
Murphy
J R
JR
D017000:Campylobacter coli / Q000145:classification / Q000302:isolation & purification* / Q000502:physiology; D016123:Campylobacter jejuni / Q000145:classification / Q000302:isolation & purification* / Q000502:physiology; D004269:DNA, Bacterial / Q000032:analysis* / Q000302:isolation & purification*; D004534:Egypt; D019286:Endopeptidase K / Q000494:pharmacology; D006361:Heating; D016133:Polymerase Chain Reaction / Q000379:methods*; D012967:Sodium Dodecyl Sulfate / Q000494:pharmacology; D014867:Water
D004269:DNA, Bacterial; D014867:Water; D012967:Sodium Dodecyl Sulfate; D019286:Endopeptidase K