title: Qualitative and semi-quantitative value of a modified 13C-urea breath test for identification of Helicobacter pylori infection.
abstract: OBJECTIVE
  The 13C-urea breath test (13C-UBT) is the method of choice in evaluating the success of therapy for eradication of Helicobacter pylori infection. For reasons of cost efficiency and practicability, urea dose and measurement duration have been reduced and the DOB (delta over baseline) cutoff level with the highest predictive value determined. Further, the efficacy of the modified 13C-UBT as a semi-quantitative test method was evaluated by comparison with histologically determined bacterial infiltration.
  
  
  METHODS
  In a prospective study, a modified 13C-UBT with reduced urea dose (75 mg) and shortened measurement duration (30 min) was administered to 145 patients. The DOB cutoff with the highest predictive value was determined using relative operating characteristic analysis. Reference methods included histology, bacterial culture and the rapid urease test. The DOB value was compared with the histologically determined grade of bacterial infiltration and the correlation evaluated using the Spearman ranking method.
  
  
  RESULTS
  Reduction of the DOB cutoff level from 5.0 per thousand to 3.5 per thousand led to significant improvement in sensitivity (78.9% vs. 91.2%) and accuracy (88.6% vs. 90.2%) of the 13C-UBT. Only five of 57 infected patients were incorrectly reported as non-infected after modification of the DOB level. In two of three patients in whom histological findings were negative but the 13C-urease test positive, bacterial growth was observed at culture. The DOB level correlated significantly with histological grade of bacterial infection.
  
  
  CONCLUSIONS
  The modified 13C-UBT proved to be a sensitive, practicable and cost-effective method for detecting H. pylori infection and permits a semi-quantitative estimation of bacterial infiltration.
authors.affiliation: Department of Gastroenterology, University of Ulm, Germany.
authors.forename: V
authors.initials: V
authors.lastname: Ellenrieder
authors.forename: B
authors.initials: B
authors.lastname: Glasbrenner
authors.forename: C
authors.initials: C
authors.lastname: Stoffels
authors.forename: S
authors.initials: S
authors.lastname: Weiler
authors.forename: G
authors.initials: G
authors.lastname: Bode
authors.forename: P
authors.initials: P
authors.lastname: Möller
authors.forename: G
authors.initials: G
authors.lastname: Adler
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D001431:Bacteriological Techniques; D001944:Breath Tests / Q000379:methods*; D002247:Carbon Isotopes; D005260:Female; D016481:Helicobacter Infections / Q000175:diagnosis*; D016480:Helicobacter pylori / Q000302:isolation & purification / Q000378:metabolism*; D006651:Histocytochemistry; D006801:Humans; D008297:Male; D008875:Middle Aged; D011237:Predictive Value of Tests; D011446:Prospective Studies; D011706:Pyloric Antrum / Q000382:microbiology; D012680:Sensitivity and Specificity; D013270:Stomach / Q000382:microbiology; D014508:Urea / Q000378:metabolism*; D014510:Urease / Q000378:metabolism
chemical_list: D002247:Carbon Isotopes; D014508:Urea; D014510:Urease