Immunomodulatory activity of G-actin fragments containing a thymopentin-like sequence.
A discontinuous thymopoietin-like motif, composed of the fragments 97-111 and 277-307 of the molecule, as well as of residues Arg178 and Asn163 of G-actin was found. It was established that G-actin has an immunosuppressive activity regarding the humoral immune response. This activity is probably connected to the thymopentin-like sequence RKDLY, which is present in the 277-307 fragment of G-actin. The immunomodulatory activity of a series of peptide-partial sequences of G-actin was tested using plaque-forming cells (PFC) and delayed type hypersensitivity (DTH) tests. The investigated series consisted of five peptides: RKDLY (I), RKDLYANT (II), DVDIRKDLY (III), DVDIR (NO2)KDLY (IV), DVDIRKDLYANT (V). The peptides have the immunosuppressive activity regarding the humoral and cellular immune response.
Siemion
I Z
IZ
Faculty of Chemistry, University of Wrocław, Poland.
Strug
I
I
Wysłouch-Cieszyńska
A
A
Nowak
E
E
Wieczorek
Z
Z
D000199:Actins / Q000276:immunology*; D000276:Adjuvants, Immunologic / Q000008:administration & dosage / Q000138:chemical synthesis / Q000502:physiology*; D000595:Amino Acid Sequence; D000818:Animals; D000917:Antibody Formation / Q000187:drug effects; D002478:Cells, Cultured; D004912:Erythrocytes / Q000276:immunology; D006829:Hydra / Q000737:chemistry; D006968:Hypersensitivity, Delayed / Q000209:etiology; D007111:Immunity, Cellular / Q000187:drug effects; D007274:Injections, Intraperitoneal; D051379:Mice; D008808:Mice, Inbred CBA; D008969:Molecular Sequence Data; D018482:Muscle, Skeletal / Q000737:chemistry; D010446:Peptide Fragments / Q000008:administration & dosage / Q000138:chemical synthesis / Q000276:immunology*; D011817:Rabbits; D012756:Sheep / Q000276:immunology; D016305:Thymopentin / Q000008:administration & dosage / Q000138:chemical synthesis / Q000276:immunology*
D000199:Actins; D000276:Adjuvants, Immunologic; D010446:Peptide Fragments; D016305:Thymopentin