Effects of a hydrogenated isomaltooligosaccharide mixture on glucan synthesis and on caries development in rats.
The caries inhibitory effect of the hydrogenated derivative of an isomaltooligosaccharides mixture (IMO-H) was examined in vitro and in vivo experiments. IMO-H could not be used as a substrate for the crude glucosyltransferases (GTases) of Streptococcus sobrinus 6715 to synthesize water-insoluble glucan. Moreover, it not only significantly inhibited the synthesis of water-insoluble glucan from sucrose, but also the sucrose-dependent adherence of these growing cells the glass surfaces. In the in vivo experiment, the addition of IMO-H to a sucrose-containing diet resulted in significant reduction of caries development in specific-pathogen-free (SPF) rats infected with S. sobrinus 6715.
Tsunehiro
J
J
Research and Development Center, Showa Sangyo Co., Ltd., Ibaraki, Japan.
Matsukubo
T
T
Shiota
M
M
Takaesu
Y
Y
D000818:Animals; D001422:Bacterial Adhesion / Q000187:drug effects; D002327:Cariostatic Agents / Q000494:pharmacology*; D003731:Dental Caries / Q000382:microbiology / Q000517:prevention & control*; D019422:Dietary Sucrose / Q000009:adverse effects; D005936:Glucans / Q000096:biosynthesis*; D005964:Glucosyltransferases / Q000378:metabolism; D066298:In Vitro Techniques; D007534:Isomaltose / Q000494:pharmacology*; D009844:Oligosaccharides / Q000494:pharmacology*; D051381:Rats; D013047:Specific Pathogen-Free Organisms; D013291:Streptococcus / Q000378:metabolism
D002327:Cariostatic Agents; D019422:Dietary Sucrose; D005936:Glucans; D009844:Oligosaccharides; D007534:Isomaltose; D005964:Glucosyltransferases