Ca2+/CaM-sensitive adenylyl cyclase activity is decreased in the Alzheimer's brain: possible relation to type I adenylyl cyclase.
Immunoreactivities of four subtypes of adenylyl cyclase (AC) (types I, II, IV and V/VI), and basal, forskolin- and Mn(2+)-stimulated AC activities with or without calcium and calmodulin (Ca2+/CaM) were estimated in parietal cortex membranes from cases with dementia of the Alzheimer type (DAT) and age-matched controls. Immunoreactivities of AC-I and AC-II were significantly decreased, but those of AC-IV and AC-V/VI did not change in DAT brains. There was a significant correlation of AC-I immunoreactivity with Ca2+/CaM-sensitive AC activity, but not with the Ca2+/CaM-insensitive activity. Ca2+/CaM-sensitive AC activity was significantly lower in DAT than in the control, indicating that impairment of Ca2+/CaM-sensitive AC-I is clearly involved in the pathophysiology of DAT.
Yamamoto
M
M
Department of Neuropsychiatry, School of Medicine, Sapporo Medical University, Japan.
Ozawa
H
H
Saito
T
T
Hatta
S
S
Riederer
P
P
Takahata
N
N
D000262:Adenylyl Cyclases / Q000378:metabolism*; D000368:Aged; D000369:Aged, 80 and over; D000544:Alzheimer Disease / Q000201:enzymology*; D001921:Brain / Q000201:enzymology*; D006801:Humans; D007150:Immunohistochemistry; D016015:Logistic Models; D008565:Membrane Proteins; D008875:Middle Aged; D009419:Nerve Tissue Proteins; D009473:Neuronal Plasticity / Q000502:physiology; D010296:Parietal Lobe / Q000201:enzymology / Q000648:ultrastructure; D013570:Synaptic Membranes / Q000648:ultrastructure
D008565:Membrane Proteins; D009419:Nerve Tissue Proteins; D000262:Adenylyl Cyclases; C106670:adenylyl cyclase 1