title: Effects of insulin on subcellular localization of hexokinase II in human skeletal muscle in vivo.
abstract: The phosphorylation of glucose to glucose-6-phosphate, catalyzed by hexokinase, is the first committed step in glucose uptake into skeletal muscle. Two isoforms of hexokinase, HKI and HKII, are expressed in human skeletal muscle, but only HKII expression is regulated by insulin. HKII messenger RNA, protein, and activity are increased after 4 h of insulin infusion; however, glucose uptake is stimulated much more rapidly, occurring within minutes. Studies in rat muscle suggest that changes in the subcellular distribution of HKII may be an important regulatory factor for glucose uptake. The present studies were undertaken to determine if insulin causes an acute redistribution of HKII activity in human skeletal muscle in vivo. Muscle biopsies (vastus lateralis muscle) were performed before and at the end of 30 min insulin infusion, performed using the euglycemic clamp technique. Muscle biopsies were subfractionated into soluble and particulate fractions to determine if insulin acutely changes the subcellular distribution of HKII. Insulin decreased HKII activity in the soluble fraction from 2.20 +/- 0.31 to 1.40 +/- 0.18 pmoles/(min[chempt]micrograms) and increased HKII activity in the particulate fraction from 3.02 +/- 0.46 to 3.45 +/- 0.46 pmoles/(min[chempt]micrograms) (P < 0.01 for both). These changes in HKII activity were correlated with changes in HKII protein, as determined by immunoblot analysis (r = 0.53, P = 0.05). Insulin had no effect on the subcellular distribution of HKII activity, which was primarily restricted to the soluble fraction. These studies are consistent with the conclusion that, in vivo in human skeletal muscle, insulin changes the subcellular distribution of HKII within 30 min.
authors.affiliation: Department of Medicine, University of Texas Health Science Center at San Antonio 78284, USA.
authors.forename: C
authors.initials: C
authors.lastname: Vogt
authors.forename: H
authors.initials: H
authors.lastname: Yki-Jarvinen
authors.forename: P
authors.initials: P
authors.lastname: Iozzo
authors.forename: R
authors.initials: R
authors.lastname: Pipek
authors.forename: M
authors.initials: M
authors.lastname: Pendergrass
authors.forename: J
authors.initials: J
authors.lastname: Koval
authors.forename: H
authors.initials: H
authors.lastname: Ardehali
authors.forename: R
authors.initials: R
authors.lastname: Printz
authors.forename: D
authors.initials: D
authors.lastname: Granner
authors.forename: R
authors.initials: R
authors.lastname: Defronzo
authors.forename: L
authors.initials: L
authors.lastname: Mandarino
mesh_terms: D000328:Adult; D000595:Amino Acid Sequence; D000818:Animals; D001706:Biopsy; D005260:Female; D015309:Glucose Clamp Technique; D006593:Hexokinase / Q000187:drug effects / Q000378:metabolism*; D006801:Humans; D015151:Immunoblotting; D007328:Insulin / Q000494:pharmacology*; D007527:Isoenzymes / Q000378:metabolism; D007700:Kinetics; D008297:Male; D008969:Molecular Sequence Data; D018482:Muscle, Skeletal / Q000166:cytology / Q000201:enzymology*; D010446:Peptide Fragments / Q000737:chemistry / Q000276:immunology; D051381:Rats; D012016:Reference Values; D013347:Subcellular Fractions / Q000201:enzymology
chemical_list: D007328:Insulin; D007527:Isoenzymes; D010446:Peptide Fragments; D006593:Hexokinase