[The effect of heparin on the course of some organ complications in experimental acute pancreatitis in rats].
The aim of this study was to present whether the heparin has on influence on course of experimental acute pancreatitis (AP) and some organ complications. The research referred to histological changes in liver, lungs and kidneys. The study was conducted on 69 rats divided into 5 groups: I--operated, without induction AP, II--control, induction of AP without treatment, III--induction of AP and immediate administration of heparin, IV--induction of AP and administration of heparin 3 hours after, V--induction of AP and administration of heparin 6 hours after. AP was induced by administration 5% sodium taurocholate. In all groups the animals were anesthetised after the period of 3, 6 and 12 hours from the induction of AP respectively. The specimens of mentioned organs were formalinized and stained with hematoxylin/eosin, and in addition specimens of the liver with p.a.S, Sudan IV and Brachets methods. In group I we observed pulmonary oedema after 6 and 12 hours but kidneys and liver were unchanged. In group II pathological changes were most evident in lungs, while we observed on acute passive hyperaemia, pulmonary emphysema and single coagulative thrombosis in vessels. In the liver number of lining cells was increased and minor or major droplets of lipids in hepatocytes were present. Using Brachets method we revealed regular staining of hepatocytes, and p.a.S. method regular staining of their cytoplasm. In group III examined organs do not show any significant differences as compared with group II, except for lungs where the coagulative thrombosis was not present. In groups IV and V changes were similar to those described in group II. This study showed decreased inflammatory and thrombotic process in lungs in experimental AP in rats after heparin treatment.
Dryka
T
T
I Katedry Chirurgii, I Kliniki Chirurgii Ogólnej i Transplantacyjnej Akademii Medycznej w Lublinie.
Radwańska-Korzeniowska
U
U
Jesipowicz
M
M
Hermanowicz-Dryka
T
T
Bacher
H
H
D000208:Acute Disease; D000818:Animals; D004334:Drug Administration Schedule; D006493:Heparin / Q000494:pharmacology*; D007275:Injections, Intravenous; D007668:Kidney / Q000187:drug effects / Q000473:pathology; D008099:Liver / Q000187:drug effects / Q000473:pathology; D008168:Lung / Q000187:drug effects* / Q000473:pathology; D008297:Male; D010195:Pancreatitis / Q000139:chemically induced*; D051381:Rats; D017208:Rats, Wistar; D013656:Taurocholic Acid
D013656:Taurocholic Acid; D006493:Heparin