title: Correlation of house dust mite-specific lymphocyte proliferation with IL-5 production, eosinophilia, and the severity of symptoms in infants with atopic dermatitis.
abstract: Because house dust mite (HDM)-specific IgE antibody (IgE-RAST) is usually not detectable in infants with atopic dermatitis (AD), HDM has not been regarded as the cause of infantile AD. The level of HDM-specific lymphocyte proliferation (expressed as stimulation index measured by flow cytometry [SIF]), however, was found to be markedly elevated in AD infants. This suggests that the sensitization of T cells to HDM extract occurs even in infancy. Moreover, the level of HDM-SIF is correlated closely with the severity of infantile AD, suggesting that HDM is a major cause of this disease not only in adults and children but also in infants. Although the level of HDM-SIF did not correlate with the level of HDM-specific IgE-RAST in infants with AD, it did intimately correlate with the absolute number of peripheral blood eosinophils. Because T lymphocytes are known to secrete some cytokines, such as IL-5, that enhance the proliferation of eosinophils, we measured the IL-5 production by peripheral blood mononuclear cells in infants with AD on stimulation with HDM extract. The amount of IL-5 production is significantly higher in infants with AD, as well as in children with AD, than that found in nonatopic control subjects. Moreover, the level of IL-5 production is correlated closely with the level of HDM-SIF in infants with AD. Taken together, these results suggest that HDMs play an important role in the development of infantile AD by inducing IL-5 production from HDM-specific T lymphocytes.
authors.affiliation: Department of Allergy and Clinical Immunology, Shizuoka Children's Hospital, Urushiyama, Shizuoka City, Japan.
authors.forename: M
authors.initials: M
authors.lastname: Kimura
authors.forename: S
authors.initials: S
authors.lastname: Tsuruta
authors.forename: T
authors.initials: T
authors.lastname: Yoshida
mesh_terms: D000293:Adolescent; D000328:Adult; D000367:Age Factors; D000485:Allergens / Q000009:adverse effects; D000818:Animals; D002176:Candida albicans / Q000276:immunology; D002648:Child; D002675:Child, Preschool; D003876:Dermatitis, Atopic / Q000209:etiology* / Q000276:immunology; D004391:Dust / Q000009:adverse effects*; D004802:Eosinophilia / Q000209:etiology* / Q000276:immunology; D006801:Humans; D007073:Immunoglobulin E / Q000097:blood; D066298:In Vitro Techniques; D007223:Infant; D015848:Interleukin-5 / Q000096:biosynthesis*; D008213:Lymphocyte Activation; D008925:Mites / Q000276:immunology*
chemical_list: D000485:Allergens; D004391:Dust; D015848:Interleukin-5; D007073:Immunoglobulin E