title: In vitro characterization of the anti-human cytomegalovirus activity of PMEA (Adefovir).
abstract: PMEA [9-[2-(phosphonomethoxy)ethyl]adenine; adefovir] has shown anti-cytomegalovirus activity in animal models and in preliminary human trials. PMEA diphosphate (PMEApp), the active antiviral metabolite of PMEA, is a potent inhibitor of human cytomegalovirus (HCMV) DNA polymerase. PMEA is efficiently taken up and phosphorylated to PMEApp in numerous human cell lines. In vitro replication of wild type and drug resistant HCMV clinical isolates is effectively inhibited by PMEA. PMEA in combination with other anti-HCMV agents shows additive inhibition of HCMV replication.
authors.affiliation: Gilead Sciences, Foster City, CA 94404, USA.
authors.forename: X
authors.initials: X
authors.lastname: Xiong
authors.forename: C
authors.initials: C
authors.lastname: Flores
authors.forename: M D
authors.initials: MD
authors.lastname: Fuller
authors.forename: D B
authors.initials: DB
authors.lastname: Mendel
authors.forename: A S
authors.initials: AS
authors.lastname: Mulato
authors.forename: K
authors.initials: K
authors.lastname: Moon
authors.forename: M S
authors.initials: MS
authors.lastname: Chen
authors.forename: J M
authors.initials: JM
authors.lastname: Cherrington
mesh_terms: D000225:Adenine / Q000031:analogs & derivatives* / Q000494:pharmacology; D000998:Antiviral Agents / Q000494:pharmacology*; D002460:Cell Line; D003587:Cytomegalovirus / Q000187:drug effects* / Q000235:genetics; D006367:HeLa Cells; D006801:Humans; D063065:Organophosphonates
chemical_list: D000998:Antiviral Agents; D063065:Organophosphonates; C053001:adefovir; D000225:Adenine