Proadrenomedullin N-terminal 20-peptide (PAMP) receptors and signal transduction in the rat adrenal gland.
Using ligand binding techniques we have demonstrated specific saturable binding of proadrenomedullin N-terminal 20-peptide (PAMP) in the rat adrenal zona glomerulosa. Hill plot analysis revealed two classes of receptor (Kd1 1.9nmol/l Bmax 53fmol/mg protein: Kd2 10nmol/l, Bmax 225fmol/mg). [125 I]-PAMP was displaced by adrenomedullin at one of these receptors. In the adrenal medulla [125 I]-PAMP bound to a single class of specific receptor (Kd 4.9 nmol/l: Bmax 556fmol/mg). PAMP caused an increase in cAMP generation by zona glomerulosa cells, but not inner zone/medulla cells. These data suggest that PAMP acts through a specific receptor to elevate cAMP levels in the rat adrenal.
Hinson
J P
JP
Department of Biochemistry, Faculty of Basic Medical Sciences, Queen Mary and Westfield College, London, UK. j.hinson@qmw.ac.uk
Hagi-Pavli
E
E
Thomson
L M
LM
Kapas
S
S
D000311:Adrenal Glands / Q000033:anatomy & histology / Q000502:physiology*; D053607:Adrenomedullin; D000818:Animals; D000242:Cyclic AMP / Q000502:physiology; D010446:Peptide Fragments / Q000502:physiology*; D010455:Peptides / Q000737:chemistry / Q000378:metabolism; D011506:Proteins / Q000502:physiology*; D051381:Rats; D017208:Rats, Wistar; D011956:Receptors, Cell Surface / Q000502:physiology*; D015398:Signal Transduction
D010446:Peptide Fragments; D010455:Peptides; D011506:Proteins; D011956:Receptors, Cell Surface; D053607:Adrenomedullin; D000242:Cyclic AMP