title: Multiubiquitin chain binding and protein degradation are mediated by distinct domains within the 26 S proteasome subunit Mcb1.
abstract: The 26 S proteasome is a multisubunit proteolytic complex responsible for degrading eukaryotic proteins targeted by ubiquitin modification. Substrate recognition by the complex is presumed to be mediated by one or more common receptor(s) with affinity for multiubiquitin chains, especially those internally linked through lysine 48. We have identified previously a candidate for one such receptor from diverse species, designated here as Mcb1 for Multiubiquitin chain-binding protein, based on its ability to bind Lys48-linked multiubiquitin chains and its location within the 26 S proteasome complex. Even though Mcb1 is likely not the only receptor in yeast, it is necessary for conferring resistance to amino acid analogs and for degrading a subset of ubiquitin pathway substrates such as ubiquitin-Pro-beta-galactosidase (Ub-Pro-beta-gal) (van Nocker, S., Sadis, S., Rubin, D.M., Glickman, M., Fu, H., Coux, O., Wefes, I., Finley, D., and Vierstra, R. D. (1996) Mol. Cell. Biol. 16, 6020-28). To further define the role of Mcb1 in substrate recognition by the 26 S proteasome, a structure/function analysis of various deletion and site-directed mutants of yeast and Arabidopsis Mcb1 was performed. From these studies, we identified a single stretch of conserved hydrophobic amino acids (LAM/LALRL/V (ScMcb1 228-234 and At-Mcb1 226-232)) within the C-terminal half of each polypeptide that is necessary for interaction with Lys48-linked multiubiquitin chains. Unexpectedly, this domain was not essential for either Ub-Pro-beta-gal degradation or conferring resistance to amino acid analogs. The domain responsible for these two activities was mapped to a conserved region near the N terminus. Yeast and Arabidopsis Mcb1 derivatives containing an intact multiubiquitin-binding site but missing the N-terminal region failed to promote Ub-Pro-beta-gal degradation and even accentuated the sensitivity of the yeast delta mcb1 strain to amino acid analogs. This hypersensitivity was not caused by a gross defect in 26 S proteasome assembly as mutants missing either the N-terminal domain or the multiubiquitin chain-binding site could still associate with 26 S proteasome and generate a complex indistinguishable in size from that present in wild-type yeast. Together, these data indicate that residues near the N terminus, and not the multiubiquitin chain-binding site, are most critical for Mcb1 function in vivo.
authors.affiliation: Cellular and Molecular Biology Program, University of Wisconsin-Madison 53706, USA.
authors.forename: H
authors.initials: H
authors.lastname: Fu
authors.forename: S
authors.initials: S
authors.lastname: Sadis
authors.forename: D M
authors.initials: DM
authors.lastname: Rubin
authors.forename: M
authors.initials: M
authors.lastname: Glickman
authors.forename: S
authors.initials: S
authors.lastname: van Nocker
authors.forename: D
authors.initials: D
authors.lastname: Finley
authors.forename: R D
authors.initials: RD
authors.lastname: Vierstra
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D017360:Arabidopsis; D029681:Arabidopsis Proteins; D001483:Base Sequence; D001665:Binding Sites; D002352:Carrier Proteins / Q000737:chemistry* / Q000235:genetics; D016384:Consensus Sequence; D004252:DNA Mutational Analysis; D017931:DNA Primers; D004330:Drosophila; D005656:Fungal Proteins / Q000737:chemistry / Q000235:genetics; D006801:Humans; D008969:Molecular Sequence Data; D010447:Peptide Hydrolases / Q000737:chemistry*; D016133:Polymerase Chain Reaction; D046988:Proteasome Endopeptidase Complex; D015003:Yeasts
chemical_list: D029681:Arabidopsis Proteins; D002352:Carrier Proteins; D017931:DNA Primers; D005656:Fungal Proteins; C097202:MBP1 protein, Arabidopsis; D010447:Peptide Hydrolases; D046988:Proteasome Endopeptidase Complex; C061553:ATP dependent 26S protease