Inhibition of fibroblast-populated collagen lattice contraction by an albumin-bound lipid fraction in human amniotic fluid.
Human amniotic fluid inhibits the contraction of fibroblast-populated collagen lattices. Amniotic fluid of 21 weeks' gestation was previously reported to have peak inhibiting activity. An attempt to isolate the inhibitory factor(s) from human amniotic fluid employing a molecular sieving column demonstrated an inhibitory factor near 70,000 molecular weight (repeated three times with triplicate samples). Since albumin has a molecular weight of 68,000 and is in high concentration in amniotic fluid, the removal of albumin by an Affi-Gel Blue (an albumin binding affinity column) was the first step in the purification scheme. The bound human amniotic fluid albumin fraction was eluted from the Affi-Gel Blue column, and the unexpected finding was that this fraction inhibited fibroblast-populated collagen lattice contraction (three repetitions in duplicate). We previously showed that prostaglandins and saturated and unsaturated fatty acids affect fibroblast-populated collagen lattice contraction, and albumin is a carrier for lipids. The eluted albumin fraction was separated into its protein and lipid components. The lipid-free albumin fraction had no fibroblast-populated collagen lattice contraction inhibiting activity (run four times in duplicate). The isolated lipid fraction inhibited fibroblast-populated collagen lattice contraction in a dose-responsive manner (repeated twice in triplicate). It was demonstrated that lipid bound to albumin isolated from human amniotic fluid inhibited fibroblast-populated collagen lattice contraction.
Yager
J S
JS
Division of Plastic and Reconstructive Surgery, Columbia University Presbyterian Medical Center, New York, NY, USA.
Hugo
N E
NE
Ehrlich
H P
HP
D000418:Albumins / Q000032:analysis; D000653:Amniotic Fluid / Q000737:chemistry / Q000502:physiology*; D002478:Cells, Cultured; D002921:Cicatrix / Q000517:prevention & control*; D003094:Collagen / Q000502:physiology*; D005347:Fibroblasts; D006801:Humans; D008055:Lipids / Q000032:analysis*; D014945:Wound Healing / Q000502:physiology*
D000418:Albumins; D008055:Lipids; D003094:Collagen