title: Stimulation by urocortin of growth hormone (GH) secretion in GH-producing human pituitary adenoma cells.
abstract: Urocortin (Ucn) possesses high homology with CRH and is considered to be a ligand to type-2 CRH receptor. We investigated the effect of Ucn on hormone release from cultured GH-producing human pituitary adenoma cells in vitro. GH-producing human pituitary adenoma cells were superfused on a Sephadex G-25 column. Both Ucn (10 nM) and CRH (10 nM) elicited an increase in GH release from the pituitary adenoma cells in one patient with acromegaly. In contrast, GH release from the pituitary adenoma cells was stimulated by Ucn but not by CRH in the other patient with acromegaly. These preliminary findings suggest that type-2 CRH receptors are expressed in some population of GH-producing human pituitary adenoma cells and that Ucn might be involved in GH secretion from tumorous tissues in patients with acromegaly.
authors.affiliation: Department of Medicine, Shimane Medical University, Izumo, Japan.
authors.forename: Y
authors.initials: Y
authors.lastname: Murakami
authors.forename: T
authors.initials: T
authors.lastname: Mori
authors.forename: K
authors.initials: K
authors.lastname: Koshimura
authors.forename: M
authors.initials: M
authors.lastname: Kurosaki
authors.forename: T
authors.initials: T
authors.lastname: Hori
authors.forename: N
authors.initials: N
authors.lastname: Yanaihara
authors.forename: Y
authors.initials: Y
authors.lastname: Kato
mesh_terms: D000236:Adenoma / Q000378:metabolism* / Q000473:pathology; D003346:Corticotropin-Releasing Hormone / Q000494:pharmacology*; D005260:Female; D019382:Human Growth Hormone / Q000378:metabolism*; D006801:Humans; D008875:Middle Aged; D010911:Pituitary Neoplasms / Q000378:metabolism* / Q000473:pathology; D012636:Secretory Rate / Q000187:drug effects; D013268:Stimulation, Chemical; D014407:Tumor Cells, Cultured; D054832:Urocortins
chemical_list: D054832:Urocortins; D019382:Human Growth Hormone; D003346:Corticotropin-Releasing Hormone