title: Polymerase chain reaction-based assays of vitreous samples for the diagnosis of viral retinitis. Use in diagnostic dilemmas.
abstract: OBJECTIVE
  This study aimed to review the authors' results using polymerase chain reaction (PCR)-based assays for the diagnosis of viral retinitis.
  
  
  METHODS
  The study design was a retrospective case series.
  
  
  METHODS
  Thirty-seven patients (38 eyes) with active retinitis from whom vitreous biopsy specimens were received in the authors' laboratory for diagnostic evaluation.
  
  
  METHODS
  Vitreous biopsy specimens were evaluated with previously described PCR-based assays for cytomegalovirus (CMV), varicella zoster virus (VZV), and herpes simplex virus (HSV) DNA; clinical histories were reviewed.
  
  
  METHODS
  Laboratory findings and clinical course were measured.
  
  
  RESULTS
  The results of the authors' assays were consistent with the long-term clinical course of each patient. Cytomegalovirus, VZV, or HSV DNA was detected in the vitreous from 24 patients. Cytomegalovirus DNA was detected in vitreous biopsy specimens from 10 patients (11 eyes). Nine patients (ten eyes) with acquired immune deficiency syndrome ultimately were diagnosed with CMV retinitis as they were followed clinically over time. Varicella zoster virus DNA was detected in vitreous biopsy specimens from eight patients; seven adult patients were ultimately diagnosed with acute retinal necrosis or progressive outer retinal necrosis. Herpes simplex virus DNA was detected in vitreous biopsy specimens from six patients; five patients had previous or subsequent herpes encephalitis. No viral DNA was detected in the vitreous from 13 patients; all were ultimately diagnosed with toxoplasmosis, syphilis, Behcet disease, fungal endophthalmitis, or idiopathic inflammation.
  
  
  CONCLUSIONS
  These data further support the use of PCR-based assays of vitreous specimens in the diagnostic evaluation of patients with infectious retinitis.
authors.affiliation: Francis I. Proctor Foundation, University of California, San Francisco 94122-0944, USA.
authors.forename: C M
authors.initials: CM
authors.lastname: Knox
authors.forename: D
authors.initials: D
authors.lastname: Chandler
authors.forename: G A
authors.initials: GA
authors.lastname: Short
authors.forename: T P
authors.initials: TP
authors.lastname: Margolis
mesh_terms: D017088:AIDS-Related Opportunistic Infections / Q000175:diagnosis; D000328:Adult; D000368:Aged; D002648:Child; D003587:Cytomegalovirus / Q000235:genetics / Q000302:isolation & purification; D017726:Cytomegalovirus Retinitis / Q000175:diagnosis*; D017931:DNA Primers / Q000737:chemistry; D004279:DNA, Viral / Q000032:analysis / Q000302:isolation & purification; D015828:Eye Infections, Viral / Q000175:diagnosis; D005654:Fundus Oculi; D006561:Herpes Simplex / Q000175:diagnosis*; D006563:Herpes Zoster Ophthalmicus / Q000175:diagnosis*; D018259:Herpesvirus 1, Human / Q000235:genetics / Q000302:isolation & purification; D014645:Herpesvirus 3, Human / Q000235:genetics / Q000302:isolation & purification; D006801:Humans; D008875:Middle Aged; D016133:Polymerase Chain Reaction / Q000379:methods*; D012160:Retina / Q000473:pathology; D012173:Retinitis / Q000175:diagnosis* / Q000821:virology; D012189:Retrospective Studies; D014822:Vitreous Body / Q000821:virology*
chemical_list: D017931:DNA Primers; D004279:DNA, Viral