Determination of tolterodine and the 5-hydroxymethyl metabolite in plasma, serum and urine using gas chromatography-mass spectrometry.
A specific and sensitive capillary gas chromatography-mass spectrometry assay for the determination of tolterodine and the 5-hydroxymethyl metabolite (Labcode DD 01) in plasma, serum and urine is described. Extraction of the analytes was performed with liquid/liquid or solid-phase extraction prior to derivatisation with a silyl reagent. The derivatives were quantified by selected ion monitoring mass spectrometry using deuterium-labelled internal standards. A single level calibration curve was utilised for quantification of plasma, serum and urine concentrations of tolterodine and DD 01. The accuracy (inter- and intra-day) for both analytes was within 87-110% in the range 0.5 and 50 ng ml-1 and precision was better than 90%. Overall, this method was shown to be reliable for pharmacokinetic assays of tolterodine and the metabolite DD 01 in samples from preclinical and clinical studies.
Palmér
L
L
Department of Bioanalytical Chemistry, Pharmacia & Upjohn AB, Uppsala, Sweden.
Andersson
L
L
Andersson
T
T
Stenberg
U
U
D001559:Benzhydryl Compounds / Q000097:blood* / Q000493:pharmacokinetics / Q000652:urine*; D002138:Calibration; D003408:Cresols / Q000097:blood* / Q000493:pharmacokinetics / Q000652:urine*; D004355:Drug Stability; D008401:Gas Chromatography-Mass Spectrometry; D006801:Humans; D018727:Muscarinic Antagonists / Q000097:blood* / Q000493:pharmacokinetics / Q000652:urine*; D010665:Phenylpropanolamine; D012044:Regression Analysis; D015203:Reproducibility of Results; D000068737:Tolterodine Tartrate
C109909:5-hydroxymethyl tolterodine; D001559:Benzhydryl Compounds; D003408:Cresols; D018727:Muscarinic Antagonists; D010665:Phenylpropanolamine; D000068737:Tolterodine Tartrate