Localization of two forms of phospholipase C-beta1, a and b, in C6Bu-1 cells.
Phospholipase C-beta1 (PLC-beta1), one of the PLC-beta isozymes, exists as two immunologically distinguishable polypeptides of 150 (PLC-beta1a) and 140 kDa (PLC-beta1b) which are encoded in two distinct transcripts and generated by alternative splicing of a single gene. In this study, the subcellular localization of the two phospholipases C-beta1 proteins was examined in rat C6Bu-1 glioma cells using immunological techniques. Immunoblot analysis revealed that the two forms of PLC-beta1 were detectable in both cytosolic and nuclear fractions. PLC-beta1a appeared to be located preferentially in the cytosol, whereas PLC-beta1b was found predominantly in the nuclei of C6Bu-1 cells. Immunocytochemical experiments confirmed the differential localization of the two PLC-beta1 species in C6Bu-1 cells. These results suggest that the two PLC-beta1 proteins may have different physiological roles in the cell.
Bahk
Y Y
YY
Department of Life Science, Pohang University of Science and Technology, Kyungbuk, South Korea.
Song
H
H
Baek
S H
SH
Park
B Y
BY
Kim
H
H
Ryu
S H
SH
Suh
P G
PG
D017398:Alternative Splicing; D000818:Animals; D000906:Antibodies / Q000276:immunology; D015153:Blotting, Western; D002458:Cell Fractionation; D002467:Cell Nucleus / Q000201:enzymology*; D003600:Cytosol / Q000201:enzymology*; D005910:Glioma; D015151:Immunoblotting; D007150:Immunohistochemistry; D007527:Isoenzymes / Q000032:analysis* / Q000737:chemistry / Q000276:immunology; D054799:Phospholipase C beta; D051381:Rats; D014407:Tumor Cells, Cultured; D010738:Type C Phospholipases / Q000032:analysis* / Q000737:chemistry / Q000276:immunology
D000906:Antibodies; D007527:Isoenzymes; D010738:Type C Phospholipases; D054799:Phospholipase C beta; C518035:Plcb1 protein, rat