Molecular cloning of a splice variant of Caenorhabditis elegans YNK1, a putative element in signal transduction.
YNK1 is a 98.3-kDa protein whose sequence was originally deduced from a genomic sequence in Caenorhabditis elegans. It was recently found that YNK1 is homologous to three different proteins implicated in signal transduction, suggesting that YNK1 is a signal transduction protein. In this report we describe the isolation of a full-length cDNA that encodes a splice variant of YNK1, designated YNK1a. We also present evidence that both YNK1 and YNK1a transcripts exist in vivo. Furthermore, using an antibody raised against a YNK1a recombinant protein, we demonstrate that the YNK1 protein is expressed in vivo throughout development.
Che
S
S
Department of Clinical Investigation, The University of Texas, M.D. Anderson Cancer Center, Houston 77030, USA.
Weil
M M
MM
Etkin
L D
LD
Epstein
H F
HF
Kuang
J
J
D000818:Animals; D017173:Caenorhabditis elegans / Q000235:genetics*; D029742:Caenorhabditis elegans Proteins; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000302:isolation & purification; D018507:Gene Expression Regulation, Developmental; D017238:Genes, Helminth; D005801:Genes, Homeobox; D015801:Helminth Proteins / Q000096:biosynthesis / Q000737:chemistry / Q000235:genetics; D018398:Homeodomain Proteins / Q000096:biosynthesis / Q000737:chemistry / Q000235:genetics; D012326:RNA Splicing; D012333:RNA, Messenger / Q000737:chemistry; D017421:Sequence Analysis; D017386:Sequence Homology, Amino Acid; D015398:Signal Transduction / Q000235:genetics*; D014158:Transcription, Genetic
C109773:ALX-1 protein, C elegans; D029742:Caenorhabditis elegans Proteins; D018076:DNA, Complementary; D015801:Helminth Proteins; D018398:Homeodomain Proteins; D012333:RNA, Messenger