title: Cloning and analysis of the human complement factor H gene promoter.
abstract: The 5' flanking region of human factor H was cloned using nested polymerase chain reaction (PCR) and the promoter finder method. A total of 1.2 kb has been sequenced and a number of putative regulatory elements identified including glucocorticoid response elements cAMP responsive element, HTF-1, and acute phase signal sequences. A 717 b.p. fragment was cloned into a CAT reporter vector and transfected into HeLa cells. A series of truncations from the 5' end of this fragment were also cloned into the CAT vector. Analysis of CAT activity of the cell lysates showed that the region from -699 to +18 is likely to contain promoter elements for the factor H gene as it was able to drive transcription of the CAT gene.
authors.affiliation: Department of Microbiology and Infectious Diseases, Flinders Medical Centre, Bedford Park, Australia. helena.ward@flinders.edu.au
authors.forename: H M
authors.initials: HM
authors.lastname: Ward
authors.forename: N H
authors.initials: NH
authors.lastname: Higgs
authors.forename: T K
authors.initials: TK
authors.lastname: Blackmore
authors.forename: T A
authors.initials: TA
authors.lastname: Sadlon
authors.forename: D L
authors.initials: DL
authors.lastname: Gordon
mesh_terms: D001483:Base Sequence; D003001:Cloning, Molecular; D017242:Complement Factor H / Q000235:genetics*; D018076:DNA, Complementary / Q000302:isolation & purification; D006801:Humans; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D011401:Promoter Regions, Genetic / Q000235:genetics*; D017422:Sequence Analysis, DNA
chemical_list: C493495:CFH protein, human; D018076:DNA, Complementary; D017242:Complement Factor H