title: Myelin basic protein-related proteins in mouse brain and immune tissues.
abstract: Brain and immune system tissues express myelin basic protein (MBP) mRNAs that contain novel exons upstream of those of the classic MBPs. We have generated antisera against a recombinant protein that includes the deduced sequence for one of the predicted species of the new MBP-related protein, hemopoietic HMBPR1. We report here the presence of multiple proteins reactive to the antisera in a range of tissues. The principal finding is that the antisera recognize a family of proteins of approximately 25 kDa that are restricted to brain, thymus, and spleen, the only tissues in which the new MBP-related transcripts are present. These antisera also detect other proteins of apparent molecular mass consistent with other isoforms of predicted MBP-related proteins. The expression of MBP-related proteins in immune system tissues may be important for self-tolerance to CNS MBPs and the initiation of immune-associated demyelinating diseases.
authors.affiliation: CNRS UPR 9035, Développement et Immunité du Système Nerveux Central, Paris, France.
authors.forename: S
authors.initials: S
authors.lastname: Kalwy
authors.forename: M C
authors.initials: MC
authors.lastname: Marty
authors.forename: P
authors.initials: P
authors.lastname: Bausero
authors.forename: B
authors.initials: B
authors.lastname: Pessac
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D015153:Blotting, Western; D001921:Brain / Q000378:metabolism*; D005260:Female; D007106:Immune Sera / Q000276:immunology; D007107:Immune System / Q000378:metabolism*; D008297:Male; D051379:Mice; D004676:Myelin Basic Protein / Q000235:genetics / Q000276:immunology / Q000378:metabolism*; D012333:RNA, Messenger / Q000378:metabolism; D013154:Spleen / Q000378:metabolism; D013950:Thymus Gland / Q000378:metabolism
chemical_list: D007106:Immune Sera; D004676:Myelin Basic Protein; D012333:RNA, Messenger