The TRI11 gene of Fusarium sporotrichioides encodes a cytochrome P-450 monooxygenase required for C-15 hydroxylation in trichothecene biosynthesis.
Several genes in the trichothecene biosynthetic pathway of Fusarium sporotrichioides have been shown to reside in a gene cluster. Sequence analysis of a cloned DNA fragment located 3.8 kb downstream from TRI5 has led to the identification of the TRI11 gene. The nucleotide sequence of TRI11 predicts a polypeptide of 492 residues (Mr = 55,579) with significant similarity to members of the cytochrome P-450 superfamily. TRI11 is most similar to several fungal cytochromes P-450 (23 to 27% identity) but is sufficiently distinct to define a new cytochrome P-450 gene family, designated CYP65A1. Disruption of TRI11 results in an altered trichothecene production phenotype characterized by the accumulation of isotrichodermin, a trichothecene pathway intermediate. The evidence suggests that TRI11 encodes a C-15 hydroxylase involved in trichothecene biosynthesis.
Alexander
N J
NJ
Mycotoxin Research Unit, National Center for Agricultural Utilization Research, U.S. Department of Agriculture/Agricultural Research Service, Peoria, Illinois 61604, USA.
Hohn
T M
TM
McCormick
S P
SP
D000595:Amino Acid Sequence; D002874:Chromosome Mapping; D003001:Cloning, Molecular; D003360:Cosmids; D003577:Cytochrome P-450 Enzyme System / Q000235:genetics* / Q000378:metabolism; D004271:DNA, Fungal / Q000032:analysis / Q000235:genetics*; D005670:Fusarium / Q000201:enzymology* / Q000235:genetics* / Q000378:metabolism; D005800:Genes, Fungal; D006900:Hydroxylation; D008969:Molecular Sequence Data; D005810:Multigene Family; D016133:Polymerase Chain Reaction; D015183:Restriction Mapping; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D014170:Transformation, Genetic; D014255:Trichothecenes / Q000096:biosynthesis* / Q000235:genetics / Q000378:metabolism
D004271:DNA, Fungal; D014255:Trichothecenes; D003577:Cytochrome P-450 Enzyme System; C063750:isotrichodermin