A sulfatide receptor distinct from L-selectin is involved in lymphocyte activation.
Sulfatide induces leukocyte activation, which is thought to be mediated via L-selectin. Here we demonstrate that lymphocytes express a receptor for sulfatide distinct from L-selectin and that this receptor is involved in sulfatide-induced cell activation. While cell surface L-selectin expression was abolished by phorbol 12-myristate 13-acetate (PMA), lymphocytes retained the ability to bind sulfatide in liquid phase as well as in immobilized solid phase. The novel sulfatide receptor obtained from PMA-treated lymphocytes showed a molecular size of 65 kDa. Stimulation through this receptor triggered cytosolic free Ca2+ elevation and intercellular aggregation.
Ding
Z
Z
Department of Bioregulation, Biomedical Research Center, Osaka University Medical School, Suita, Japan.
Kawashima
H
H
Suzuki
Y
Y
Suzuki
T
T
Ward
P A
PA
Miyasaka
M
M
D000818:Animals; D001665:Binding Sites; D002118:Calcium / Q000378:metabolism; D019041:L-Selectin / Q000378:metabolism; D008213:Lymphocyte Activation; D008214:Lymphocytes / Q000276:immunology / Q000378:metabolism*; D008297:Male; D051381:Rats; D017208:Rats, Wistar; D016201:Receptors, Lymphocyte Homing / Q000378:metabolism*; D013433:Sulfoglycosphingolipids / Q000378:metabolism*
D016201:Receptors, Lymphocyte Homing; D013433:Sulfoglycosphingolipids; D019041:L-Selectin; D002118:Calcium