Purification of Pf155/RESA antigen from supernatants of in vitro Plasmodium falciparum cultures by continuous elution electrophoresis.
The malaria parasite Plasmodium falciparum synthesizes a protein, Pf155/RESA, which associates with the membrane of newly invaded erythrocytes. Using spent supernatants from P. falciparum growing in culture as a source of soluble Pf155/RESA, we have developed a purification technique based on the concentration of these supernatants, followed by gel filtration and continuous elution electrophoresis. SDS-PAGE electrophoresis and immunoblots were used to establish the quality of the purification.
Dubois
B
B
Institut National de la Santé et de la Recherche Médicale (INSERM) Unité 13, Paris, France.
Deloron
P
P
D000818:Animals; D000953:Antigens, Protozoan / Q000302:isolation & purification*; D000954:Antigens, Surface / Q000302:isolation & purification*; D002850:Chromatography, Gel; D003470:Culture Media / Q000737:chemistry; D004591:Electrophoresis, Polyacrylamide Gel; D015151:Immunoblotting; D010963:Plasmodium falciparum / Q000737:chemistry / Q000276:immunology*; D015800:Protozoan Proteins / Q000302:isolation & purification*
D000953:Antigens, Protozoan; D000954:Antigens, Surface; D003470:Culture Media; D015800:Protozoan Proteins; C050256:ring-infected erythrocyte surface antigen (RESA), Plasmodium falciparum