Emergence of an irreversible differentiated subclone from the poorly differentiated ovarian adenocarcinoma cell line AMOC-2 treated with sodium butyrate.
OBJECTIVE
To cast light on the capacity of an ovarian adenocarcinoma to undergo irreversible cellular differentiation, we established clonal cell lines from cultured ovarian carcinoma cells treated with sodium butyrate and examined their phenotypic changes.


METHODS
The poorly differentiated ovarian carcinoma cell line AMOC-2 was exposed to 2 mM sodium butyrate for 30 days, and clonal cell lines were established by a dilution plating technique.


RESULTS
Five clonal cell lines were established. One of them had a longer doubling time and lower plating efficiency than the parental clone, also demonstrating stronger expression of vimentin and 56 kD cytokeratin. This clone became columnar with pronounced polarity, formed gland-like structures when cultured in collagen gels, and exhibited no tumorigenicity, in contrast to the parental clone.


CONCLUSIONS
Sodium butyrate treatment of AMOC-2 cells can cause phenotypic changes reminiscent of maturation in the Müllerian duct, endocervix, and/or endometrium.
Yabushita
H
H
Department of Obstetrics and Gynecology, Aichi Medical University, Japan.
Noguchi
M
M
Nakanishi
M
M
D000230:Adenocarcinoma / Q000737:chemistry / Q000473:pathology*; D000818:Animals; D015153:Blotting, Western; D002087:Butyrates / Q000494:pharmacology*; D020148:Butyric Acid; D002452:Cell Count; D002454:Cell Differentiation; D002455:Cell Division / Q000187:drug effects; D003094:Collagen; D003470:Culture Media; D005260:Female; D006801:Humans; D007633:Keratins / Q000032:analysis; D051379:Mice; D008819:Mice, Nude; D009368:Neoplasm Transplantation; D010051:Ovarian Neoplasms / Q000737:chemistry / Q000473:pathology*; D013997:Time Factors; D014407:Tumor Cells, Cultured; D014746:Vimentin / Q000032:analysis
D002087:Butyrates; D003470:Culture Media; D014746:Vimentin; D020148:Butyric Acid; D007633:Keratins; D003094:Collagen