[Adsorption of superoxide dismutase and catalase from microemulsions of surface active agents in heptane].
Superoxide dismutase (SOD) and catalase were solubilized in heptane microemulsions stabilized with an aerosol of sodium salt of sulfosuccinic acid bis(2-ethylhexyl) ester or its mixture with Triton X-45. The efficiencies of six methods of isolation of the enzymes from the microemulsions were compared. The methods used were based on the degradation of microheterogeneous systems induced by acetone, aqueous saline solution, sorbents, or combinations of these agents. Isolation procedures based on hydroxyapatite or silica gel sorbents yield SOD preparations with fully retained catalytic activity and catalase preparations with enhanced activity and operating stability.
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D000818:Animals; D002374:Catalase / Q000737:chemistry / Q000302:isolation & purification*; D002417:Cattle; D004655:Emulsions; D006536:Heptanes; D008099:Liver / Q000201:enzymology*; D013482:Superoxide Dismutase / Q000737:chemistry / Q000302:isolation & purification*; D013501:Surface-Active Agents
D004655:Emulsions; D006536:Heptanes; D013501:Surface-Active Agents; D002374:Catalase; D013482:Superoxide Dismutase