GCN5, a yeast transcriptional coactivator, induces chromatin reconfiguration of HIS3 promoter in vivo.
Gcn5p, the nuclear histone acetyltransferase (HAT A), is a component of the multiprotein adaptor complex, ADA. Its role as a transcriptional coactivator is required for full induction of most of the genes regulated by GCN4. In this study we present experimental evidence demonstrating that, during gene activation, the nuclease sensitive region of HIS3 promoter, harbouring the poly (dA:dT) and the GCN4 binding site, is invaded by nucleosomes in a gcn5 disrupted strain. These data demonstrate, for the first time, that Gcn5p affects directly the chromatin organization of a chromosomal gene during its transcriptional activation.
Filetici
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Centro di studio per gli Acidi Nucleici, CNR, Dipartimento di Genetica e Biologia Molecolare, Università La Sapienza, Rome, Italy.
Aranda
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C
Gonzàlez
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A
Ballario
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P
D002843:Chromatin / Q000648:ultrastructure*; D004271:DNA, Fungal; D004268:DNA-Binding Proteins; D005656:Fungal Proteins / Q000235:genetics / Q000378:metabolism*; D015966:Gene Expression Regulation, Fungal; D051548:Histone Acetyltransferases; D006836:Hydro-Lyases / Q000235:genetics*; D008957:Models, Genetic; D009154:Mutation; D009707:Nucleosomes; D011401:Promoter Regions, Genetic; D011494:Protein Kinases / Q000235:genetics / Q000378:metabolism*; D029701:Saccharomyces cerevisiae Proteins; D014157:Transcription Factors / Q000378:metabolism*; D015533:Transcriptional Activation; D015003:Yeasts; D000099224:p300-CBP-Associated Factor
D002843:Chromatin; D004271:DNA, Fungal; D004268:DNA-Binding Proteins; D005656:Fungal Proteins; D051548:Histone Acetyltransferases; D006836:Hydro-Lyases; D009707:Nucleosomes; D011494:Protein Kinases; D029701:Saccharomyces cerevisiae Proteins; D014157:Transcription Factors; C501392:GCN5 protein, S cerevisiae; D000099224:p300-CBP-Associated Factor; C021434:imidazoleglycerolphosphate dehydratase