Quantification of the full length leptin receptor (OB-Rb) in human brown and white adipose tissue.
Levels of expression of the leptin receptor (OB-R) splice variants have been studied in human omental white and perirenal brown adipose tissues by reverse transcription-PCR. The level of mRNA expression of the full length form (OB-Rb) was approximately 15% of that of the sum of all splice variants in white or brown adipose tissue. In an attempt to quantify the gene expression of OB-Rb in human white adipose tissue, a quantitative competitive PCR technique was developed, using oligonucleotide primers designed for OB-Rb and an internal standard for a "MIMIC" competition strategy. The levels of expression of OB-Rb mRNA in the omental fat of lean and obese patients were compared and no difference could be observed between the two groups. The quantitative RT-PCR technique allows for a fast and accurate measurement of the expression of the OB-Rb mRNA in small tissue samples.
Kutoh
E
E
Department of Medical Biochemistry, CMU, Geneva, Switzerland.
Boss
O
O
Levasseur
F
F
Giacobino
J P
JP
D000273:Adipose Tissue / Q000502:physiology*; D002001:Adipose Tissue, Brown / Q000502:physiology*; D000328:Adult; D017398:Alternative Splicing; D002352:Carrier Proteins / Q000235:genetics*; D015870:Gene Expression; D006801:Humans; D008297:Male; D008875:Middle Aged; D009765:Obesity / Q000378:metabolism*; D016133:Polymerase Chain Reaction; D011956:Receptors, Cell Surface; D054411:Receptors, Leptin
D002352:Carrier Proteins; C515448:LEPR protein, human; D011956:Receptors, Cell Surface; D054411:Receptors, Leptin