title: Effects of chitinolytic and proteolytic enzymes on in vitro phagocytosis of microsporidians by spleen macrophages of turbot, Scophthalmus maximus L.
abstract: The serum of many teleosts, including turbot, contains chitinolytic and proteolytic enzymes. In the present study, the possible role of these enzymes in nonspecific immune responses to microsporidian infection was investigated. The rate of phagocytosis of Glugea caulleryi spores by turbot splenic macrophages was significantly reduced after pretreatment of spores with proteolytic or chitinolytic enzymes, suggesting that alteration of surface glycoproteins affects spore recognition. However, intracellular superoxide production by macrophages was significantly higher after stimulation with protease-treated spores, or with untreated spores plus normal turbot serum (NTS), than after stimulation with untreated spores in the absence of NTS. These results support the view that the chitinolytic and proteolytic activities in teleost serum may play a role in defence against microsporidian infection.
authors.affiliation: Instituto de Investigación y Análisis Alimentarios, Facultad de Farmacia, Universidad de Santiago de Compostela, Spain. mpleiro@use.es
authors.forename: J
authors.initials: J
authors.lastname: Leiro
authors.forename: M
authors.initials: M
authors.lastname: Ortega
authors.forename: M I
authors.initials: MI
authors.lastname: Siso
authors.forename: M L
authors.initials: ML
authors.lastname: Sanmartín
authors.forename: F M
authors.initials: FM
authors.lastname: Ubeira
mesh_terms: D000818:Animals; D002688:Chitinases / Q000097:blood / Q000494:pharmacology*; D005412:Flatfishes / Q000097:blood; D008264:Macrophages / Q000502:physiology*; D016816:Microsporida / Q000187:drug effects*; D009113:Muramidase / Q000097:blood / Q000494:pharmacology*; D010587:Phagocytosis / Q000187:drug effects / Q000502:physiology*; D013154:Spleen / Q000166:cytology*; D013170:Spores / Q000187:drug effects; D013481:Superoxides / Q000378:metabolism; D014357:Trypsin / Q000097:blood / Q000494:pharmacology*
chemical_list: D013481:Superoxides; D002688:Chitinases; D009113:Muramidase; D014357:Trypsin