Marginal zinc deficiency lowers the lymphatic absorption of alpha-tocopherol in rats.
The present study was conducted to investigate whether the intestinal absorption of vitamin E is influenced by marginal zinc deficiency. Rats trained to meal feed were divided into two groups and fed a diet containing 3 mg Zn/kg [a low zinc (LZ group)] or pair-fed (PF controls a zinc-adequate diet (30 mg Zn/kg). At 5 wk, the body weight (352 +/- 5 g, mean +/- SD) of LZ rats was 98.5% of that of PF rats (357 +/- 8 g). Rats with lymph cannula were infused at 3 mL/h via a duodenal catheter with a lipid emulsion consisting of 568 micromol triolein, 3.56 micromol alpha-tocopherol (alphaTP) and 396 micromol Na+-taurocholate in 24 mL of phosphate-buffered saline (pH 6.4). Lymph was collected hourly for 8 h. The amounts of alphaTP absorbed into the lymph were determined by high-performance liquid chromatography (HPLC). The hourly rate of alphaTP absorption was significantly lower in LZ than in PF rats. A marked difference (P < 0.05) was clearly evident even at 1 h (1.8 +/- 1.2 nmol/h in LZ vs. 8.5 +/- 3.0 nmol/h in PF). The peak rate of absorption was significantly lower in LZ rats (67.1 +/- 16.7 nmol/h at 5 h) than in PF rats (95.9 +/- 7.7 nmol/h at 4 h). The total amounts of alphaTP absorbed in 8 h in LZ and PF rats were 391.1 +/- 54.4 nmol (11.0 +/- 1.5% dose) and 613.9 +/- 105.8 nmol (17.2 +/- 3.0% dose), respectively. The lymphatic absorption of alphaTP was correlated with the amounts of PL (r = 0.77, P < 0.05) released into the mesenteric lymph. The hourly outputs of phospholipid and oleic acid also were significantly lower in LZ rats than in PF rats up to 4 h (P < 0.05). The cumulative lymphatic outputs of phospholipid (PL) were 20.1 +/- 3.7 micromol/8 h in LZ and 27.0 +/- 3.9 micromol/8 h in PF rats (P < 0.05). These results show that the intestinal absorption of vitamin E is affected by the zinc status of rats. This observation along with our earlier finding of a lower intestinal absorption of retinol suggests that zinc nutriture has a profound effect on the intestinal absorption and body status of lipid soluble vitamins.
Kim
E S
ES
Department of Foods and Nutrition, Kansas State University, Manhattan, KS 66506, USA.
Noh
S K
SK
Koo
S I
SI
D000818:Animals; D004032:Diet; D007408:Intestinal Absorption / Q000187:drug effects*; D008099:Liver / Q000187:drug effects / Q000378:metabolism; D008208:Lymphatic System / Q000187:drug effects* / Q000378:metabolism; D008297:Male; D019301:Oleic Acid / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D014810:Vitamin E / Q000097:blood / Q000493:pharmacokinetics*; D015032:Zinc / Q000097:blood / Q000172:deficiency* / Q000494:pharmacology*
D014810:Vitamin E; D019301:Oleic Acid; D015032:Zinc