title: Ripped pocket and pickpocket, novel Drosophila DEG/ENaC subunits expressed in early development and in mechanosensory neurons.
abstract: Drosophila melanogaster has proven to be a good model for understanding the physiology of ion channels. We identified two novel Drosophila DEG/ ENaC proteins, Pickpocket (PPK) and Ripped Pocket (RPK). Both appear to be ion channel subunits. Expression of RPK generated multimeric Na+ channels that were dominantly activated by a mutation associated with neurodegeneration. Amiloride and gadolinium, which block mechanosensation in vivo, inhibited RPK channels. Although PPK did not form channels on its own, it associated with and reduced the current generated by a related human brain Na+ channel. RPK transcripts were abundant in early stage embryos, suggesting a role in development. In contrast, PPK was found in sensory dendrites of a subset of peripheral neurons in late stage embryos and early larvae. In insects, such multiple dendritic neurons play key roles in touch sensation and proprioception and their morphology resembles human mechanosensory free nerve endings. These results suggest that PPK may be a channel subunit involved in mechanosensation.
authors.affiliation: Howard Hughes Medical Institute, Department of Internal Medicine, University of Iowa College of Medicine, Iowa City, Iowa 52242, USA.
authors.forename: C M
authors.initials: CM
authors.lastname: Adams
authors.forename: M G
authors.initials: MG
authors.lastname: Anderson
authors.forename: D G
authors.initials: DG
authors.lastname: Motto
authors.forename: M P
authors.initials: MP
authors.lastname: Price
authors.forename: W A
authors.initials: WA
authors.lastname: Johnson
authors.forename: M J
authors.initials: MJ
authors.lastname: Welsh
mesh_terms: D000584:Amiloride / Q000494:pharmacology; D000595:Amino Acid Sequence; D000818:Animals; D001921:Brain / Q000502:physiology; D003001:Cloning, Molecular; D004331:Drosophila melanogaster / Q000196:embryology; D004625:Embryo, Nonmammalian / Q000502:physiology; D005682:Gadolinium / Q000494:pharmacology; D018507:Gene Expression Regulation, Developmental; D006801:Humans; D008969:Molecular Sequence Data; D005810:Multigene Family; D009475:Neurons, Afferent / Q000187:drug effects / Q000502:physiology*; D010802:Phylogeny; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000737:chemistry; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid; D015222:Sodium Channels / Q000096:biosynthesis* / Q000737:chemistry / Q000502:physiology
chemical_list: D011994:Recombinant Proteins; D015222:Sodium Channels; D000584:Amiloride; D005682:Gadolinium