Effect of atrial natriuretic peptide on sodium-glucose cotransport in the rat small intestine.
Atrial natriuretic peptide (ANP) decreases sodium absorption in small intestine of rats in vitro under sodium concentration-gradient conditions (SCG) and this effect may be mediated by the inhibition of the sodium/glucose cotransporter (SGLT). In order to assess this hypothesis, the effects of ANP, phloridzine (Phlz) and methylene blue (MB), added alone or together, using a voltage clamp technique in Ussing's chamber with SCG were studied. ANP and Phlz significantly decreased potential difference and short circuit current. Effects of Phlz and ANP were not additive. The addition of MB alone did not affect ion transport, whereas it abolished ANP effects. These data suggest that ANP blocks the SGLT through mechanisms mediated by cGMP and/or NO.
González Bosc
L V
LV
Departamento de Ciencias Biológicas, Facultad de Farmacia y Bioquímica, UBA, Buenos Aires, Argentina.
Elustondo
P A
PA
Ortiz
M C
MC
Vidal
N A
NA
D000818:Animals; D009320:Atrial Natriuretic Factor / Q000494:pharmacology*; D001692:Biological Transport / Q000187:drug effects; D006152:Cyclic GMP / Q000378:metabolism; D004553:Electric Conductivity; D007421:Intestine, Small / Q000187:drug effects* / Q000378:metabolism; D008297:Male; D008751:Methylene Blue / Q000494:pharmacology; D009004:Monosaccharide Transport Proteins / Q000037:antagonists & inhibitors* / Q000378:metabolism; D009569:Nitric Oxide / Q000378:metabolism; D018408:Patch-Clamp Techniques; D010695:Phlorhizin / Q000494:pharmacology; D051381:Rats; D017208:Rats, Wistar; D012964:Sodium / Q000494:pharmacology
D009004:Monosaccharide Transport Proteins; D009569:Nitric Oxide; D009320:Atrial Natriuretic Factor; D012964:Sodium; D010695:Phlorhizin; D006152:Cyclic GMP; D008751:Methylene Blue