Rainbow trout SOX9: cDNA cloning, gene structure and expression.
We have isolated and characterized rainbow trout SOX9 cDNA and genomic clones. The cDNA encodes a protein of 488 amino acids (aa) with an HMG box and has 70% aa sequence identity with human SOX9. The rainbow trout and human SOX9 genes show a similar gene structure, and the two introns in the coding region are located at conserved positions. In rainbow trout, the SOX9 mRNA was prominent in testis and brain.
Takamatsu
N
N
Department of Biosciences, School of Science, Kitasato University, Sagamihara, Kanagawa, Japan. takamatu@jet.sci.kitasato-u.ac.jp
Kanda
H
H
Ito
M
M
Yamashita
A
A
Yamashita
S
S
Shiba
T
T
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D015152:Blotting, Northern; D002645:Chickens; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000737:chemistry / Q000302:isolation & purification*; D005786:Gene Expression Regulation; D006609:High Mobility Group Proteins / Q000096:biosynthesis / Q000235:genetics*; D006801:Humans; D008297:Male; D051379:Mice; D008969:Molecular Sequence Data; D017686:Oncorhynchus mykiss / Q000235:genetics*; D012333:RNA, Messenger / Q000032:analysis; D055758:SOX9 Transcription Factor; D012733:Sex Differentiation / Q000235:genetics*; D013737:Testis; D014157:Transcription Factors / Q000096:biosynthesis / Q000235:genetics*
D018076:DNA, Complementary; D006609:High Mobility Group Proteins; D012333:RNA, Messenger; D055758:SOX9 Transcription Factor; C527932:SOX9 protein, human; C527933:Sox9 protein, mouse; D014157:Transcription Factors