title: A novel gene of tomato preferentially expressed in fruit encodes a protein with a Ca2+-dependent lipid-binding domain.
abstract: A cDNA clone, called CLB1, was isolated from a cDNA library from tomato (Lycopersicon esculentum) and characterized. The CLB1 cDNA contains an open reading frame of 1518 bp, and encodes a putative protein of 506 amino acids with a predicted molecular mass of 54,633 Da. The deduced CLB1 amino acid sequence contains a domain that exhibits from 26% to 37% identity with the Ca2+-dependent lipid-binding domains of cytosolic phospholipase A2, protein kinase C, Rabphilin-3A, and Synaptotagmin 1 of animals. Southern blot analysis indicates that the CLB1 gene belongs to a small gene family in the tomato genome. The CLB1 mRNA is preferentially expressed in fruit tissues.
authors.affiliation: Institute of Biological Chemistry, Washington State University, Pullman 99164-6340, USA.
authors.forename: T
authors.initials: T
authors.lastname: Kiyosue
authors.forename: C A
authors.initials: CA
authors.lastname: Ryan
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D015152:Blotting, Northern; D015139:Blotting, Southern; D002118:Calcium / Q000502:physiology*; D002352:Carrier Proteins / Q000096:biosynthesis / Q000235:genetics*; D018076:DNA, Complementary / Q000302:isolation & purification; D005638:Fruit / Q000235:genetics* / Q000378:metabolism; D018506:Gene Expression Regulation, Plant; D018551:Solanum lycopersicum / Q000235:genetics* / Q000378:metabolism; D008969:Molecular Sequence Data; D010940:Plant Proteins / Q000096:biosynthesis / Q000235:genetics*
chemical_list: C109818:CLB1 protein, Lycopersicon esculentum; D002352:Carrier Proteins; D018076:DNA, Complementary; D010940:Plant Proteins; D002118:Calcium