Pharmacomanipulation of HSV-1 induced chorioretinitis in mice.
In the von Szily mouse model, intracameral inoculation of herpes simplex virus type-1 (HSV-1) results in inflammation of the ipsilateral anterior segment with relative chorioretinal sparing and destructive contralateral chorioretinitis. We studied the effect of the systemic antiviral agent acyclovir (ACV) and anti-HSV-1 antibody therapy in this model. Contralateral chorioretinitis developed in none of the 18 mice receiving ACV from post-inoculation day (pid) 1 (p < 0.0001), in 6 of 10 (60%) mice when treatment was delayed until pid 7 (p = 0.40) and in 14 of 18 (77%) controls. Contralateral disease developed in 8 of 16 (50%) mice that received anti-HSV-1 antibody from pid 1 (p = 0.02), in 13 of 16 (81%) treated from pid 5 (p = 0.64), in 7 of 8 (87.5%) treated from pid 7 (p = 1.0) and in 17 of 20 (85%) controls. We conclude that early treatment with ACV or anti-HSV-1 antibody reduces the incidence of contralateral chorioretinitis in mice.
Merchant
A
A
Hilles Immunology Laboratory, Massachusetts Eye and Ear Infirmary, Harvard Medical School, Boston 02114, USA.
Fletcher
J
J
Medina
C A
CA
Hemady
R K
RK
Foster
C S
CS
D000212:Acyclovir / Q000627:therapeutic use*; D000818:Animals; D000914:Antibodies, Viral / Q000627:therapeutic use; D000998:Antiviral Agents / Q000627:therapeutic use*; D002825:Chorioretinitis / Q000517:prevention & control* / Q000821:virology; D015828:Eye Infections, Viral / Q000517:prevention & control / Q000821:virology; D005260:Female; D006561:Herpes Simplex / Q000517:prevention & control*; D018259:Herpesvirus 1, Human / Q000276:immunology; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C
D000914:Antibodies, Viral; D000998:Antiviral Agents; D000212:Acyclovir