cDNA cloning and expression of the gene encoding murine stromelysin-2 (MMP-10).
Recently, we demonstrated a biphasic induction of the epithelial broad-spectrum matrix metalloproteinase (MMP) stromelysin-2 during cutaneous wound healing. Now we have generated a murine wound cDNA libary and have used it to isolate the putative cDNA of this murine matrix metalloproteinase. The predicted sequence of the protein shows 76 and 89% identity with its human and rat analogues, respectively. Stromelysin-2 and stromelysin-1 transcripts were both detected at very low levels in the lung and the heart of adult Balb/c mice, whereas stromelysin-2 mRNA expression alone was found at comparatively high levels in the small intestine, a tissue characterized by continuous epithelial renewal. Recombinant forms of murine stromelysin-1 and -2 produced in transfected COS cells were secreted and could be induced to undergo autocatalytic processing by addition of the organomercurial salt 4-aminophenylmercuric acetate (APMA).
Madlener
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M
Max-Plank-Institue für Biochemie, Martinsried, Germany.
Werner
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D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D019556:COS Cells; D018076:DNA, Complementary / Q000096:biosynthesis / Q000302:isolation & purification*; D005260:Female; D005786:Gene Expression Regulation; D019267:Hemagglutinin Glycoproteins, Influenza Virus / Q000235:genetics; D053506:Matrix Metalloproteinase 10; D019278:Matrix Metalloproteinase 3 / Q000187:drug effects / Q000235:genetics; D008666:Metalloendopeptidases / Q000096:biosynthesis* / Q000187:drug effects / Q000235:genetics*; D051379:Mice; D008807:Mice, Inbred BALB C; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D009928:Organ Specificity / Q000235:genetics; D010662:Phenylmercuric Acetate / Q000031:analogs & derivatives / Q000494:pharmacology; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis; D012689:Sequence Homology, Nucleic Acid
D018076:DNA, Complementary; D019267:Hemagglutinin Glycoproteins, Influenza Virus; D012333:RNA, Messenger; D011993:Recombinant Fusion Proteins; C010024:4-aminophenylmercuriacetate; D008666:Metalloendopeptidases; D019278:Matrix Metalloproteinase 3; D053506:Matrix Metalloproteinase 10; D010662:Phenylmercuric Acetate