Quantification of antigen-specific immunoglobulin A after oral booster immunization with ovalbumin in mice mono-associated with segmented filamentous bacteria or Clostridium innocuum.
Segmented filamentous bacteria (SFB) are known to stimulate the mucosal immune system. Here, the effect of SFB on oral booster immunization with ovalbumin was investigated. Mice mono-associated with SFB or Clostridium innocuum were sensitized by intraperitoneal administration of 100 micrograms ovalbumin with Freunds complete adjuvant. After 4 weeks, mice received 80 mg ovalbumin orally. A maximum IgA response was found 5 days after this booster immunization. Comparison of mice with SFB and mice with C. innocuum revealed a much higher level of IgA in the gut lumen and more IgA secreting cells in the lamina propria of the SFB-associated mice. However, no differences between both groups of animals were found in specific levels of IgA secreting cells or luminal IgA against ovalbumin. It is concluded that there is no enhancing effect of SFB after booster immunization when mice are primed intraperitoneally with ovalbumin.
Snel
J
J
Central Animal Laboratory, University of Nijmegen, The Netherlands.
Bakker
M H
MH
Heidt
P J
PJ
D000284:Administration, Oral; D000818:Animals; D000918:Antibody Specificity; D000921:Antibody-Producing Cells; D003013:Clostridium / Q000276:immunology*; D007117:Immunization, Secondary; D007070:Immunoglobulin A / Q000032:analysis / Q000276:immunology*; D007413:Intestinal Mucosa / Q000276:immunology*; D007422:Intestines / Q000276:immunology / Q000382:microbiology; D008297:Male; D051379:Mice; D010047:Ovalbumin / Q000276:immunology*
D007070:Immunoglobulin A; D010047:Ovalbumin