DNA integrity in human sperm.
Since the 1970s there have been conflicting reports of decreasing sperm counts in man and increasing testicular cancer. There is a hypothetical link between apparent adverse trends in several measures of human reproductive health and exposure to endocrine disrupters. Rodent bioassays are not suited for the large-scale screening of such chemicals because of their costs, complexity, and ethical concerns. Various in vitro assays have been used to examine the effects of these chemicals, but none has directly used semen as one of the target tissues in man. The present study has examined in the alkaline Comet assay in human sperm the effect of two estrogens--beta-estradiol and the phytoestrogen daidzein--and 1,2-epoxybutene, a metabolite of 1,3-butadiene, and compared them with the effects of the known reprotoxin, dibromochloropropane, in two fertile and two infertile frozen sperm samples and two fresh fertile samples. While differences were detected in the frozen fertile and infertile samples with flow cytometry, in the Comet assay both frozen and fresh samples exposed to the chemicals in vitro from fertile and infertile men produced similar altered responses by comparison with untreated samples. The integrity of DNA is necessary not only for the noncancerous state, but also for the accurate transmission of genetic material to the next generation. Thus this assay may be useful for examination of chemicals in fresh and frozen sperm samples.
Anderson
D
D
Department of Genetic and Reproductive Toxicology, BIBRA International, Carshalton, Surrey, United Kingdom. danderson@bibra.co.uk
Dobrzyńska
M M
MM
Yu
T W
TW
Gandini
L
L
Cordelli
E
E
Spano
M
M
D004247:DNA / Q000187:drug effects*; D004249:DNA Damage; D005434:Flow Cytometry; D006801:Humans; D007248:Infertility, Male / Q000378:metabolism; D008297:Male; D013094:Spermatozoa / Q000737:chemistry*
D004247:DNA