Time course analysis of semi-quantitative PCR and antigenaemia assay for prevention of cytomegalovirus disease after bone marrow transplantation.
Cytomegalovirus (CMV) disease is a serious complication after bone marrow transplantation (BMT). Recently, semi-quantitative polymerase chain reaction (SQ-PCR) analysis for CMV-DNA has been developed. We compared the time course of SQ-PCR and that of antigenaemia assay after BMT. Our findings suggested that SQ-PCR is more sensitive than antigenaemia assay in the detection of CMV infection and that quantitation of CMV-DNA could discriminate patients with positive PCR but without the risk for CMV disease. We considered that SQ-PCR could be a useful guide for starting pre-emptive therapy against CMV disease.
Kanda
Y
Y
Department of Cell Therapy and Transplantation Medicine, Faculty of Medicine, University of Tokyo, Japan.
Chiba
S
S
Suzuki
T
T
Kami
M
M
Yazaki
Y
Y
Hirai
H
H
D000293:Adolescent; D000328:Adult; D000956:Antigens, Viral / Q000097:blood*; D016026:Bone Marrow Transplantation / Q000009:adverse effects*; D003586:Cytomegalovirus Infections / Q000276:immunology / Q000517:prevention & control*; D005069:Evaluation Studies as Topic; D005260:Female; D015774:Ganciclovir / Q000627:therapeutic use; D006801:Humans; D008297:Male; D008875:Middle Aged; D016133:Polymerase Chain Reaction; D012698:Serologic Tests
D000956:Antigens, Viral; D015774:Ganciclovir