Principal involvement of cyclooxygenase-1-derived prostaglandins in the c-fos expression of the rat hind brain following visceral stimulation with acetic acid.
We investigated the relationship between c-fos expression in the hind brain and peritoneal prostaglandin (PG) synthesis after visceronociceptive stimulation with acetic acid in rats. Time-course studies on the mRNA expression for c-fos in the hind brain and cyclooxygenase (COX) isoforms in the peritoneal cells, as well as on the peritoneal 6-keto-PGF1alpha accumulation, after stimulation indicated that COX-1 but not COX-2 was responsible for the peritoneal synthesis of PGs which were suggested to evoke c-fos expression in the hind brain. Pharmacological experiments using mofezolac, a preferential inhibitor against COX-1, and NS-398, a selective inhibitor against COX-2, confirmed the involvement of COX-1 derived PGs in the induction of c-fos expression in the hind brain following the noxious stimulation.
Kusuhara
H
H
Research Laboratories, Yoshitomi Pharmaceutical Industries, Ltd., Koiwai, Fukuoka, Japan.
Fukunari
A
A
Matsuyuki
H
H
Okumoto
T
T
D019342:Acetic Acid / Q000494:pharmacology*; D000818:Animals; D051545:Cyclooxygenase 1; D005260:Female; D007527:Isoenzymes / Q000502:physiology*; D008565:Membrane Proteins; D011451:Prostaglandin-Endoperoxide Synthases / Q000502:physiology*; D011453:Prostaglandins / Q000096:biosynthesis; D016760:Proto-Oncogene Proteins c-fos / Q000096:biosynthesis*; D051381:Rats; D017207:Rats, Sprague-Dawley; D012249:Rhombencephalon / Q000187:drug effects* / Q000378:metabolism; D013268:Stimulation, Chemical; D014781:Viscera / Q000187:drug effects* / Q000378:metabolism
D007527:Isoenzymes; D008565:Membrane Proteins; D011453:Prostaglandins; D016760:Proto-Oncogene Proteins c-fos; D051545:Cyclooxygenase 1; D011451:Prostaglandin-Endoperoxide Synthases; C496513:Ptgs1 protein, rat; D019342:Acetic Acid