title: The single-copy gene encoding high-mobility-group protein HMG-I/Y from pea contains a single intron and is expressed in all organs.
abstract: The coding and 3'-downstream regions of the gene encoding the high mobility group protein HMG-I/Y from pea have been isolated, sequenced and characterised. A 795 bp pea genomic fragment containing the coding region of the pea HMG-I/Y gene with a single intron of 201 bp was isolated by PCR. The gene encodes a protein of 197 amino acid residues with four copies of the AT-hook DNA-binding motif encoded by exon 2. Southern blot analysis on genomic DNA revealed the presence of a single copy of the HMG-I/Y gene in the haploid genome. The pea HMG-I/Y gene is expressed in all organs of pea including roots, stems, leaves, flowers, tendrils and developing seeds, as determined by northern blot analysis.
authors.affiliation: Department of Plant Sciences, University of Cambridge, UK.
authors.forename: R
authors.initials: R
authors.lastname: Gupta
authors.forename: C I
authors.initials: CI
authors.lastname: Webster
authors.forename: J C
authors.initials: JC
authors.lastname: Gray
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D015152:Blotting, Northern; D003001:Cloning, Molecular; D018628:Gene Dosage; D018506:Gene Expression Regulation, Plant; D025741:HMGA1a Protein; D006609:High Mobility Group Proteins / Q000096:biosynthesis / Q000235:genetics* / Q000302:isolation & purification; D007438:Introns; D008969:Molecular Sequence Data; D009928:Organ Specificity / Q000235:genetics; D018532:Pisum sativum / Q000235:genetics*; D010940:Plant Proteins / Q000096:biosynthesis / Q000235:genetics* / Q000302:isolation & purification; D018520:Plant Shoots / Q000235:genetics; D016133:Polymerase Chain Reaction
chemical_list: C107092:HMGA1a Protein, plant; D006609:High Mobility Group Proteins; D010940:Plant Proteins; D025741:HMGA1a Protein