Using PRINS for gene mapping in polytene chromosomes.
We have adapted the primed in situ labelling (PRINS) protocol for gene mapping in polytene chromosomes of two dipteran species. The method was used to localize the genes for the Balbiani ring (BR) 2.1 and the iron-regulatory protein 1A (IRP1A) in polytene salivary gland chromosomes of Chironomus tentans, and Drosophila melanogaster respectively. Two oligonucleotides, corresponding to the BR 2.1 and IRP1A genes, were used as primers and the whole procedure was performed within 3-4 h. The strong labelling with low background revealed the localization of the BR 2.1 gene in polytene chromosome IV of C. tentans and the IRP1A gene in polytene chromosome 3R83 of D. melanogaster. The results demonstrated that PRINS is a fast, sensitive and suitable approach for physical gene mapping in polytene chromosomes.
Gu
H F
HF
Department of Medical Genetics, Biomedical Centre, Uppsala University, Sweden.
Lind
M I
MI
Wieslander
L
L
Landegren
U
U
Söderhäll
K
K
Melefors
O
O
D000818:Animals; D002683:Chironomidae / Q000235:genetics; D002874:Chromosome Mapping; D002875:Chromosomes / Q000235:genetics*; D017931:DNA Primers / Q000235:genetics; D004331:Drosophila melanogaster / Q000235:genetics; D017344:Genes, Insect / Q000235:genetics; D017403:In Situ Hybridization / Q000379:methods*; D019476:Insect Proteins / Q000235:genetics; D012469:Salivary Glands / Q000648:ultrastructure; D012680:Sensitivity and Specificity
D017931:DNA Primers; D019476:Insect Proteins