title: Phenotype of porcine monocytic cells: modulation of surface molecule expression upon monocyte differentiation into macrophages.
abstract: When porcine blood monocytes differentiate in vitro into macrophages, their morphology, as well as side scatter and forward scatter measured by flow cytometry, changed in a manner similar to that with human cells. During this differentiation, the initial high expression of CD molecules on porcine monocytes was down-regulated, with one exception--SWC9. Freshly isolated blood monocytes were SWC9-, but after culture the cells had become SWC9+. Thus, porcine monocytes were characterised as SWC3+SWC9-CD14high; macrophages were SWC3+SWC9+CD14low, the latter also displaying a down-regulation of CD11a/18, and, to a lesser degree, CD44. Both SWC9-CD14high monocytes and SWC9+CD14low macrophages were identifiable in freshly prepared monocytic cells from the spleen. Alveolar macrophages, on the other hand, were dominated by SWC9+CD14low cells, similar in phenotype to in vitro monocyte-derived macrophages. The consequences which these results have for studies on virus infections of porcine monocytic cells are discussed.
authors.affiliation: Institute of Virology and Immunoprophylaxis, CH-3147 Mittelhäusern, Switzerland. kenneth.mccullough@ivi.admin.ch
authors.forename: K C
authors.initials: KC
authors.lastname: McCullough
authors.forename: R
authors.initials: R
authors.lastname: Schaffner
authors.forename: V
authors.initials: V
authors.lastname: Natale
authors.forename: Y B
authors.initials: YB
authors.lastname: Kim
authors.forename: A
authors.initials: A
authors.lastname: Summerfield
mesh_terms: D000818:Animals; D015703:Antigens, CD / Q000032:analysis*; D002454:Cell Differentiation; D002478:Cells, Cultured; D008264:Macrophages / Q000276:immunology*; D009000:Monocytes / Q000276:immunology*; D010641:Phenotype; D013552:Swine / Q000276:immunology*
chemical_list: D015703:Antigens, CD