Factors associated with the mammalian RNA polymerase II holoenzyme.
The RNA polymerase II (Pol II) holoenzyme in yeast is an essential transcriptional regulatory complex which has been defined by genetic and biochemical approaches. The mammalian counterpart to this complex, however, is less well defined. Experiments herein demonstrate that, along with Pol II and SRB proteins, proteins associated with transcriptional regulation as cofactors are associated with the Pol II holoenzyme. Earlier experiments have demonstrated that the breast cancer-associated tumor suppressor BRCA1 and the CREB binding protein (CBP) were associated with the holoenzyme complex. The protein related to CBP, the E1A-associated p300 protein, is shown in these experiments to be associated with the holoenzyme complex as well as the BRG1 subunit of the chromatin remodeling SWI/SNF complex. Importantly, the Pol II holoenzyme complex does not contain some factors previously reported as stoichiometric components of the holoenzyme complex, most notably the proteins which function in repair of damaged DNA, such as PCNA, RFC and RPA. The presence of the p300 coactivator and the chromatin-modifying BRG1 protein support a role for the Pol II holoenzyme as a key target for regulation by enhancer binding proteins.
Neish
A S
AS
Division of Molecular Oncology, Department of Pathology, Brigham and Women's Hospital and Harvard Medical School, 75 Francis Street, Boston, MA 02115, USA.
Anderson
S F
SF
Schlegel
B P
BP
Wei
W
W
Parvin
J D
JD
D019313:BRCA1 Protein / Q000235:genetics / Q000378:metabolism; D050882:CREB-Binding Protein; D002846:Chromatography, Affinity / Q000379:methods; D003067:Coenzymes / Q000737:chemistry*; D004247:DNA / Q000032:analysis; D004265:DNA Helicases; D004260:DNA Repair; D050881:E1A-Associated p300 Protein; D006367:HeLa Cells; D006801:Humans; D056892:Mediator Complex; D009687:Nuclear Proteins / Q000032:analysis* / Q000378:metabolism; D017354:Point Mutation; D012313:RNA / Q000032:analysis; D012319:RNA Polymerase II / Q000737:chemistry* / Q000302:isolation & purification; D011993:Recombinant Fusion Proteins; D015534:Trans-Activators; D014157:Transcription Factors / Q000032:analysis* / Q000378:metabolism
D019313:BRCA1 Protein; D003067:Coenzymes; C098617:MED21 protein, human; D056892:Mediator Complex; D009687:Nuclear Proteins; D011993:Recombinant Fusion Proteins; D015534:Trans-Activators; D014157:Transcription Factors; D012313:RNA; D004247:DNA; D050882:CREB-Binding Protein; C494363:CREBBP protein, human; D050881:E1A-Associated p300 Protein; D012319:RNA Polymerase II; C084108:SMARCA4 protein, human; D004265:DNA Helicases