The effect of 3-aminobenzamide on thymidine incorporation in ultra-violet irradiated chick pigment epithelium cells.
The addition of 3-aminobenzamide (3-AB) to cultures of chick embryo pigmented epithelium rescues these cells after high doses of ultraviolet treatment. The addition of 3-AB prevents cells from losing pre-formed protein and DNA and stimulates thymidine incorporation by the cells after ultraviolet irradiation. Since 3-AB is an inhibitor of poly (ADP) ribosylation, these observations support the conclusion that death of these cells after ultra-violet irradiation depends upon poly (ADP) ribosylation and may be an apoptotic response.
Baich
A
A
Biology Department, Southern Illinois University Edwardsville 62026, USA.
Wanda
P E
PE
Mitchell
M D
MD
D000818:Animals; D017209:Apoptosis / Q000187:drug effects / Q000528:radiation effects; D001549:Benzamides / Q000494:pharmacology*; D002455:Cell Division / Q000187:drug effects / Q000528:radiation effects; D002642:Chick Embryo; D004847:Epithelial Cells / Q000187:drug effects* / Q000528:radiation effects*; D010857:Pigment Epithelium of Eye / Q000166:cytology / Q000187:drug effects* / Q000528:radiation effects*; D011065:Poly(ADP-ribose) Polymerases / Q000187:drug effects / Q000528:radiation effects; D011838:Radiation-Sensitizing Agents / Q000494:pharmacology*; D013936:Thymidine / Q000378:metabolism* / Q000528:radiation effects; D014466:Ultraviolet Rays
D001549:Benzamides; D011838:Radiation-Sensitizing Agents; C025160:3-aminobenzamide; D011065:Poly(ADP-ribose) Polymerases; D013936:Thymidine