title: In vitro antiarrhythmic effect of prior whole body hyperthermia: implication of catalase.
abstract: The protective effect of heat stress against mechanical dysfunction and myocardial necrosis after prolonged ischemia is well known. We have investigated whether the protective effect of heat stress extends to reperfusion arrhythmias in the isolated perfused rat heart. Rats were exposed to 20 min of 42 degrees C hyperthermia. Twenty-four h later their hearts were isolated, perfused and subjected to a 5-min period of occlusion of the left coronary artery. The incidence and duration of reperfusion arrhythmias were assessed in the 30-min reperfusion period. Prior heat stress led to a reduction in the incidence (from 100 to 60%, P</=0.05) and duration (from 611+/-251 to 62+/-51 s, P</=0.05) of ventricular tachycardia and/or fibrillation, upon reperfusion following a 5-min ischemic period. This prevention of reperfusion arrhythmias was associated with a two-fold increase in endogenous catalase activity and an enhanced heat stress protein hsp 72 and 27 expression. Catalase inhibition by 3-amino triazole (AT) abolished the antiarrhythmic effect of heat stress. The incidence (80 v 100%) and duration (691+/-238 v 989+/-242 s) of reperfusion arrhythmias were not different between the group heat shocked + AT and the group treated only with AT. On the other hand, in the presence of AT, myocardial noradrenaline release was attenuated by prior heat stress (upon stabilization: 3.9+/-0.8 compared to 9.4+/-2.1 pg/ml/g tissue, P</=0.05; upon reperfusion: 42.7+/-7.3 compared to 69.8+/-9.5 pg/ml/g tissue, P</=0.05). In conclusion, heat stress leads to protection against reperfusion arrhythmias occurring after a short ischemic insult, in the isolated rat heart. Heat stress proteins and catalase seem to be implicated in this protective effect. Finally, we have shown that in presence of AT, heat stress decreases myocardial noradrenaline release.
authors.affiliation: Faculté de Pharmacie, Université Joseph Fourier, La Tronche, 38706, France.
authors.forename: M
authors.initials: M
authors.lastname: Joyeux
authors.forename: C
authors.initials: C
authors.lastname: Ribuot
authors.forename: V
authors.initials: V
authors.lastname: Bourlier
authors.forename: J
authors.initials: J
authors.lastname: Verdetti
authors.forename: A
authors.initials: A
authors.lastname: Durand
authors.forename: M J
authors.initials: MJ
authors.lastname: Richard
authors.forename: D
authors.initials: D
authors.lastname: Godin-Ribuot
authors.forename: P
authors.initials: P
authors.lastname: Demenge
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000378:metabolism; D001145:Arrhythmias, Cardiac / Q000201:enzymology*; D002374:Catalase / Q000378:metabolism*; D005334:Fever / Q000201:enzymology*; D006360:Heat-Shock Proteins / Q000378:metabolism; D006439:Hemodynamics; D008297:Male; D009206:Myocardium / Q000201:enzymology; D009638:Norepinephrine / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D015424:Reperfusion
chemical_list: D000975:Antioxidants; D006360:Heat-Shock Proteins; D002374:Catalase; D009638:Norepinephrine