Effect of aliphatic aldehydes on the lipid peroxidation and chemiluminescence of biological systems under oxidative stress.
The effect of several aliphatic aldehydes on lipid peroxidation was evaluated by measuring the oxygen uptake rate, thiobarbituric acid-reactive products formation and the emitted visible chemiluminescence intensity. Measurements were carried out in brain homogenates and erythrocyte plasma membrane and liver microsomal fractions. In all systems studied, aldehydes (25 mmol/L) (e.g. acetaldehyde, 2,2-dimethylpropanal), increased the intensity of the luminescence associated with the oxidation process. In contrast, aldehyde incorporation decreased TBARS production and the rate of oxygen uptake. The increased luminescence intensity is explained in terms of secondary reactions of aldehyde derived free radicals. These results clearly indicate that extreme care must be exercised in the interpretation of chemiluminescence data in the presence of aldehydes.
Videla
L A
LA
Department of Biochemistry, Faculty of Medicine, University of Chile, Santiago, Chile.
Ferrer
V
V
Lissi
E A
EA
D000447:Aldehydes / Q000494:pharmacology*; D000818:Animals; D001921:Brain / Q000187:drug effects / Q000378:metabolism*; D004910:Erythrocyte Membrane / Q000187:drug effects / Q000378:metabolism*; D015227:Lipid Peroxidation / Q000187:drug effects*; D008163:Luminescent Measurements; D008297:Male; D018384:Oxidative Stress; D010101:Oxygen Consumption; D011048:Polarography / Q000379:methods; D051381:Rats; D017208:Rats, Wistar; D015203:Reproducibility of Results; D012588:Scintillation Counting / Q000379:methods; D012680:Sensitivity and Specificity; D017392:Thiobarbituric Acid Reactive Substances / Q000032:analysis
D000447:Aldehydes; D017392:Thiobarbituric Acid Reactive Substances