A method for canine nasal mucosa superfusion, in vivo; evidence that pseudomonas-induced neutrophil migration is inhibited by alpha2-adrenoceptor agonist.
RESULTS
A method for isolating and superfusing a segment of the nasal cavity in the anaesthetized dog was developed. Introduction of Pseudomonas aeruginosa supernatant (PA), which is known to induce IL-8 release, resulted in a marked time-dependent exponential increase in neutrophil recruitment to the nasal chamber. When oxymetazoline (final concentration, 10(-5) M), was added to the superfusate, the PA induced-neutrophil migration was inhibited; the addition of benzalchoniul chloride to the superfusate had no effect on the PA induced-neutrophil migration.


CONCLUSIONS
We conclude that a alpha2 adrenoceptor agonist profoundly inhibits neutrophil migration in response to bacterial products. Our novel method allows continuous introduction of stimuli and monitoring of responses in the nasal mucosa.
Stjärne
P
P
Cardiovascular Research Institute and Department of Medicine, University of California at San Francisco, 94143-0130, USA.
Agusti
C
C
Cardell
L O
LO
Wagstaff
S
S
Nadel
J A
JA
D000316:Adrenergic alpha-Agonists / Q000494:pharmacology; D000818:Animals; D002465:Cell Movement / Q000187:drug effects; D017077:Culture Media, Conditioned / Q000494:pharmacology; D004285:Dogs; D004804:Eosinophils / Q000187:drug effects / Q000378:metabolism; D004847:Epithelial Cells / Q000187:drug effects / Q000378:metabolism; D019272:Leukocyte Elastase / Q000378:metabolism; D008264:Macrophages / Q000187:drug effects / Q000378:metabolism; D009297:Nasal Mucosa / Q000502:physiology*; D009504:Neutrophils / Q000166:cytology* / Q000187:drug effects / Q000201:enzymology; D010477:Perfusion / Q000379:methods*; D009195:Peroxidase / Q000032:analysis / Q000378:metabolism; D011550:Pseudomonas aeruginosa / Q000502:physiology*
D000316:Adrenergic alpha-Agonists; D017077:Culture Media, Conditioned; D009195:Peroxidase; D019272:Leukocyte Elastase