Cloning and structural analysis of the murine GCN5L1 gene.
We recently cloned the murine 11-cis retinol dehydrogenase gene. A second gene, the murine GCN5L1 gene, was found to be situated upstream of the murine 11-cis retinol dehydrogenase gene. We have isolated and sequenced the complete coding sequence of the murine GCN5L1 gene. The distance between the 3'-end of the murine GCN5L1 gene and the 5'-end of the 11-cis retinol dehydrogenase gene is only 776 nt. The murine GCNSL1 gene consists of four exons encompassing approximately 3.5 kb of genomic DNA. Intron/exon splice sites conform to the GT/AG rule. The open reading frame consists of 375 nucleotides encoding a 14 kDa protein. The murine GCN5L1, like the human GCN5L1 protein, displays weak homology (27%) to yeast GCN5. The distance between the murine, human and bovine GCN5L1 and 11-cis retinol dehydrogenase genes appeared to be conserved.
Driessen
C A
CA
University of Nijmegen, Department of Ophthalmology, The Netherlands. c.driessen@ohk.azn.nl
Winkens
H J
HJ
Kuhlmann
L D
LD
Janssen
B P
BP
van Vugt
A H
AH
Deutman
A F
AF
Janssen
J J
JJ
D000429:Alcohol Oxidoreductases / Q000235:genetics; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002417:Cattle; D003001:Cloning, Molecular; D018076:DNA, Complementary; D008040:Genetic Linkage; D006801:Humans; D051379:Mice; D024101:Mitochondrial Proteins; D008969:Molecular Sequence Data; D009419:Nerve Tissue Proteins; D017386:Sequence Homology, Amino Acid; D015534:Trans-Activators / Q000235:genetics*
C104378:BLOC1S1 protein, human; C000619640:BLOC1S1 protein, mouse; D018076:DNA, Complementary; D024101:Mitochondrial Proteins; D009419:Nerve Tissue Proteins; D015534:Trans-Activators; D000429:Alcohol Oxidoreductases; C022520:retinol dehydrogenase