Occurrence of monoacyl-diglucosyl-diacyl-glycerol and monoacyl-bis-glycerophosphoryl-diglucosyl-diacyl-glycerol in membranes of Acholeplasma laidlawii strain B-PG9.
It is shown by thin-layer and high-performance liquid chromatography that the two membrane lipids monoacyl-diglucosyl-diacyl-glycerol (MADGlcDAG) and monoacyl-bis-glycerophosphoryl-diglucosyl-diacyl-glycerol are synthesized by Acholeplasma laidlawii strain B-PG9 when the cells are grown in two different growth media. The two lipids are also synthesized by A. laidlawii strain A-EF22 and their chemical structures have been determined previously by NMR spectroscopy. Since a reversed hexagonal phase is the only liquid-crystalline phase formed by MADGlcDAG, it is concluded that A. laidlawii strain B-PG9, in resemblance to strain A-EF22, synthesizes three membrane lipids that are able to form reversed nonlamellar phases. A comparison of the membrane lipids from the two strains shows that there is essentially one lipid from each strain that differs. However, both these lipids have common physico-chemical properties, namely the ability to form reversed nonlamellar phases. Finally, it is also shown that novel lipids may be synthesized by A. laidlawii through long-time adaptation to altered growth conditions.
Andersson
A S
AS
Department of Physical Chemistry, Umeå University, Sweden. annsofie.andersson@chem.umu.se
Rilfors
L
L
Lewis
R N
RN
McElhaney
R N
RN
Lindblom
G
G
D000128:Acholeplasma laidlawii / Q000737:chemistry* / Q000254:growth & development / Q000378:metabolism; D002462:Cell Membrane / Q000737:chemistry; D002851:Chromatography, High Pressure Liquid; D002855:Chromatography, Thin Layer; D006017:Glycolipids / Q000032:analysis* / Q000096:biosynthesis; D008563:Membrane Lipids / Q000032:analysis / Q000737:chemistry; D015394:Molecular Structure; D050178:Monoglycerides
D006017:Glycolipids; D008563:Membrane Lipids; D050178:Monoglycerides; C014571:glucosyl diacylglycerol; C089530:monoacyl bisglycerophosphoryl diglucosyl diacylglycerol; C110189:monoacyl-diglucosyl-diacyl-glycerol