title: Rapid microwave-stimulated fixation of entire prostatectomy specimens. Biomed-II MPC Study Group.
abstract: Conventional fixation of large solid surgical specimens is a slow process. Consequently, autolytic damage to tissues may occur if the fixative does not reach the central part of the specimen in time. However, as there is also a time relationship between formalin fixation and antigen masking, fixation for too long can also be detrimental. In seeking the optimum balance for fixation, microwave irradiation might be of assistance. This study set out to evaluate methods for fixing entire prostate glands within a brief period of time, using microwave-stimulated formalin fixation. The results show that entire prostates can be optimally fixed if formalin is present throughout the tissue as the temperature is increased by microwave irradiation. This is achieved by injecting the fixative into the prostate at multiple sites immediately following prostatectomy. The technique described ensures standardization of a critical step during tissue processing, leading to uniform microscopic results with both routine and immunohistochemical stains. It is a simple, rapid method, suitable for routine diagnostic use. Using this modified approach, DNA of much larger sizes can be extracted from paraffin-embedded material, which could expand the possibilities for molecular analysis.
authors.affiliation: Department of Urology, University Hospital Nijmegen, The Netherlands.
authors.forename: E T
authors.initials: ET
authors.lastname: Ruijter
authors.forename: G J
authors.initials: GJ
authors.lastname: Miller
authors.forename: T W
authors.initials: TW
authors.lastname: Aalders
authors.forename: C A
authors.initials: CA
authors.lastname: van de Kaa
authors.forename: J A
authors.initials: JA
authors.lastname: Schalken
authors.forename: F M
authors.initials: FM
authors.lastname: Debruyne
authors.forename: M E
authors.initials: ME
authors.lastname: Boon
mesh_terms: D015820:Cadherins / Q000378:metabolism; D004247:DNA / Q000032:analysis; D004587:Electrophoresis, Agar Gel; D005557:Formaldehyde; D006801:Humans; D007633:Keratins / Q000378:metabolism; D008297:Male; D008872:Microwaves; D011467:Prostate / Q000378:metabolism / Q000473:pathology*; D011468:Prostatectomy; D016707:Tissue Fixation / Q000379:methods*; D016159:Tumor Suppressor Protein p53 / Q000378:metabolism
chemical_list: D015820:Cadherins; D016159:Tumor Suppressor Protein p53; D005557:Formaldehyde; D007633:Keratins; D004247:DNA