title: Metabolic alteration in neural tissue of rat embryos exposed to beta-hydroxybutyrate during organogenesis.
abstract: Hyperketonemia has been identified as an important factor in diabetic pregnancy affecting growth and development of the offspring. In order to assess the immediate metabolic alterations in embryos caused by excess ketone bodies, we studied rat embryonic neural tissue exposed to a high concentration of beta-hydroxybutyrate in vitro. Beta-hydroxybutyrate inhibited oxygen uptake of the neural tissue of day 9 and day 10 embryos by 12.8% and 1 1.2%, but did not affect that of day 11 and day 12 tissue. In contrast, glucose utilization of the neural tissue of day 9 and day 10 embryos was not altered. However, a 30% decrease in glucose utilization was observed in the neural tissue of day 11 and day 12 embryos exposed to beta-hydroxybutyrate. Thus, beta-hydroxybutyrate induced different metabolic alterations in the embryonic neural tissue during early and late organogenesis, which suggests different modes of teratogenic action of ketone bodies in different parts of gestation.
authors.affiliation: Department of Medical Cell Biology, University of Uppsala, Sweden.
authors.forename: X
authors.initials: X
authors.lastname: Yang
authors.forename: L A
authors.initials: LA
authors.lastname: Borg
authors.forename: U J
authors.initials: UJ
authors.lastname: Eriksson
mesh_terms: D020155:3-Hydroxybutyric Acid; D000818:Animals; D004622:Embryo, Mammalian / Q000187:drug effects / Q000378:metabolism; D005314:Embryonic and Fetal Development / Q000187:drug effects; D005260:Female; D005947:Glucose / Q000378:metabolism; D006885:Hydroxybutyrates / Q000494:pharmacology*; D008297:Male; D009474:Neurons / Q000187:drug effects* / Q000378:metabolism; D010100:Oxygen / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley; D013482:Superoxide Dismutase / Q000494:pharmacology
chemical_list: D006885:Hydroxybutyrates; D013482:Superoxide Dismutase; D005947:Glucose; D010100:Oxygen; D020155:3-Hydroxybutyric Acid