title: Orphanin FQ is the major OFQ1-17-containing peptide produced in the rodent and monkey hypothalamus.
abstract: In order to investigate the processing of OFQ containing peptides in the hypothalamus we have developed a sensitive and quantitative radioimmunoassay for OFQ. We fractionated rodent and monkey hypothalamic extracts by reversed-phase high performance liquid chromatography and found that the extracts contained multiple peaks of OFQ immunoreactivity with the major peak co-eluting with synthetic OFQ1-17. Mouse hypothalamic extracts were also fractionated by SDS-PAGE to determine the apparent molecular weights of molecules containing the OFQ peptide. Multiple peaks of OFQ immunoreactivity, ranging in size from approximately 1 to 30 kilodaltons, were detected by this method. These results suggest that OFQ1-17 is processed to smaller peptides in mouse and monkey hypothalamic neurons.
authors.affiliation: Vollum Institute for Advanced Biomedical Research, Oregon Health Sciences University, Portland 97201, USA.
authors.forename: D I
authors.initials: DI
authors.lastname: Quigley
authors.forename: J
authors.initials: J
authors.lastname: McDougall
authors.forename: T
authors.initials: T
authors.lastname: Darland
authors.forename: G
authors.initials: G
authors.lastname: Zhang
authors.forename: O
authors.initials: O
authors.lastname: Ronnekliev
authors.forename: D K
authors.initials: DK
authors.lastname: Grandy
authors.forename: R G
authors.initials: RG
authors.lastname: Allen
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D002851:Chromatography, High Pressure Liquid; D004591:Electrophoresis, Polyacrylamide Gel; D005260:Female; D007031:Hypothalamus / Q000737:chemistry* / Q000166:cytology / Q000378:metabolism; D017403:In Situ Hybridization; D008253:Macaca mulatta; D008297:Male; D051379:Mice; D008969:Molecular Sequence Data; D008970:Molecular Weight; D018847:Opioid Peptides / Q000032:analysis* / Q000302:isolation & purification / Q000378:metabolism; D010446:Peptide Fragments / Q000032:analysis / Q000302:isolation & purification; D012333:RNA, Messenger / Q000032:analysis; D011863:Radioimmunoassay; D051381:Rats; D017208:Rats, Wistar; D000097629:Nociceptin
chemical_list: D018847:Opioid Peptides; D010446:Peptide Fragments; D012333:RNA, Messenger; D000097629:Nociceptin