Presence of a conserved domain of GATA transcription factors in Echinococcus granulosus.
In order to characterize GATA transcription factors in Echinococcus granulosus, a PCR-based cloning strategy was developed. Degenerate oligonucleotides were designed for the most conserved sequence in GATA proteins that include 20 amino acids of the zinc domain. A 60 bp fragment was isolated that had high homology among this sequence and those reported in other species. An analogous sequence was obtained by performing the same procedure with DNA from the free living platyhelminth Dugesia tigrina. High stringency Southern blotting experiments confirmed the presence of this sequence in the parasite genome.
Garat
B
B
Departamento de Bioquímica, Facultad de Ciencias, Universidad de la Republica, Montevideo, Uruguay.
Esperón
P
P
Martínez
C
C
Robello
C
C
Ehrlich
R
R
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D017124:Conserved Sequence; D004446:Echinococcus / Q000235:genetics*; D017238:Genes, Helminth; D015801:Helminth Proteins / Q000235:genetics*; D008969:Molecular Sequence Data; D010932:Planarians / Q000235:genetics; D016133:Polymerase Chain Reaction; D014157:Transcription Factors / Q000235:genetics*
D015801:Helminth Proteins; D014157:Transcription Factors