Pulsed-field gel electrophoresis of genomic digests demonstrates linkages among food, food handlers, and patrons in a food-borne Salmonella javiana outbreak in Massachusetts.
A total of 66 isolates of Salmonella javiana isolated from food, food handlers, and patrons that were epidemiologically linked to an outbreak of gastroenteritis were analyzed by pulsed-field gel electrophoresis. Analysis with restriction endonucleases XbaI and SpeI supported the epidemiologic association and suggested a pathway of transmission among food, food handlers, and patrons.
Lee
R
R
State Laboratory Institute, Massachusetts Department of Public Health, Boston 02130, USA.
Peppe
J
J
George
H
H
D004269:DNA, Bacterial / Q000032:analysis; D004196:Disease Outbreaks; D016521:Electrophoresis, Gel, Pulsed-Field; D005511:Food Handling; D005516:Food Microbiology; D006801:Humans; D008404:Massachusetts / Q000453:epidemiology; D012475:Salmonella / Q000302:isolation & purification*; D012478:Salmonella Food Poisoning / Q000453:epidemiology*
D004269:DNA, Bacterial