title: Structure and specific expression of a Nicotiana sylvestris putative amino-acid transporter gene in mature and in vitro germinating pollen.
abstract: We report here the structure and expression analysis of a Nicotiana sylvestris gene, called nsaap1, coding for a protein which shows significant identity to the members of the Arabidopsis thaliana amino acid permease family. The nsaap1 gene is expressed exclusively in binucleate pollen. Its transcripts begin to accumulate after first pollen mitosis and dramatically increase in mature pollen shortly before anthesis and during in vitro pollen germination. The nsaap1 5'-flanking region contains long regions homologous to the promoter region of the tobacco pollen specific eIF-4A8 translation factor. Our results support the occurrence of an amino acid remobilization in binucleate pollen. The nsaap1 gene could play a role in exchanges between the vegetative and the generative pollen cells.
authors.affiliation: Institut de Biotechnologie des Plantes, ERS 569, Université Paris XI, Orsay, France.
authors.forename: E
authors.initials: E
authors.lastname: Lalanne
authors.forename: C
authors.initials: C
authors.lastname: Mathieu
authors.forename: O
authors.initials: O
authors.lastname: Roche
authors.forename: F
authors.initials: F
authors.lastname: Vedel
authors.forename: R
authors.initials: R
authors.lastname: De Pape
mesh_terms: D000595:Amino Acid Sequence; D026905:Amino Acid Transport Systems; D018076:DNA, Complementary / Q000378:metabolism; D018628:Gene Dosage; D017343:Genes, Plant; D018745:Genome, Plant; D008565:Membrane Proteins / Q000235:genetics / Q000378:metabolism; D026901:Membrane Transport Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D008969:Molecular Sequence Data; D010940:Plant Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D010947:Plants, Toxic; D011058:Pollen / Q000201:enzymology / Q000235:genetics* / Q000254:growth & development*; D017422:Sequence Analysis, DNA; D014026:Nicotiana / Q000201:enzymology / Q000235:genetics*; D014158:Transcription, Genetic
chemical_list: D026905:Amino Acid Transport Systems; D018076:DNA, Complementary; D008565:Membrane Proteins; D026901:Membrane Transport Proteins; D010940:Plant Proteins