Simultaneous determination of imipramine and its metabolite desipramine in human plasma by capillary gas chromatography with mass-selective detection.
An analytical method for the simultaneous determination of imipramine (IMI) and its N-desmethyl metabolite, desipramine (DIMI) in human plasma by capillary gas chromatography-mass selective detection (GC-MS), with D4-imipramine (D4-IMI) and D4-desipramine (D4-DIMI) as internal standards, was developed and validated. After addition of the internal standards, the compounds were extracted from plasma at basic pH into n-heptane-isoamyl alcohol (99:1, v/v), back-extracted into acidic aqueous solution and re-extracted at basic pH into toluene. Desipramine and D4-desipramine were converted into their pentafluoropropionyl derivatives. The compounds were determined by gas chromatography using a mass selective detector at m/z 234 for IMI, m/z 238 for D4-IMI, m/z 412 for DIMI and m/z 416 for D4-DIMI. The method was applied to clinical samples.
Pommier
F
F
Novartis Pharma, Bioanalytics and Pharmacokinetics, Rueil Malmasion, France.
Sioufi
A
A
Godbillon
J
J
D000284:Administration, Oral; D000929:Antidepressive Agents, Tricyclic / Q000008:administration & dosage / Q000097:blood* / Q000737:chemistry / Q000493:pharmacokinetics; D002138:Calibration; D002849:Chromatography, Gas / Q000379:methods*; D002940:Circadian Rhythm; D003891:Desipramine / Q000097:blood* / Q000737:chemistry / Q000378:metabolism; D004355:Drug Stability; D006801:Humans; D007099:Imipramine / Q000008:administration & dosage / Q000097:blood* / Q000737:chemistry / Q000493:pharmacokinetics; D009994:Osmolar Concentration; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013997:Time Factors
D000929:Antidepressive Agents, Tricyclic; D007099:Imipramine; D003891:Desipramine