In vitro effects of recombinant human granulocyte-macrophage colony-stimulating factor (RhGM-CSF) on polymorphonuclear cell (PMN) and monocyte (MO) functional capacities in ovarian cancer patients versus human volunteers.
Human polymorphonuclear cells (PMN) and monocytes (MO) from four ovarian cancer patients and seventeen normal donors were in vitro pretreated with different concentrations (25, 50 and 100 IU, respectively) of rhGM-CSF. Phagocytosis and killing of PMN and MO as well as PMN polarization were evaluated in cancer patients before treatment (T0) and at the end of each chemotherapeutic cycle (T1, T2, T3 and T4, respectively) in comparison with normal donors. RhGM-CSF did not affect phagocytosis and killing of PMN and MO. On the other hand, this cytokine was per se endowed with the capacity to enhance PMN polarization in both cancer patients (at T2 interval) and normal donors.
Altamura
M
M
Department of Internal Medicine, Immunology and Infectious Diseases, University of Bari Medical School, Italy.
Geronimo
M G
MG
Pepe
M
M
Lella
P
P
Nappi
L
L
Loizzi
P
P
Forte
M
M
Marsilio
M
M
Piazzolla
G
G
Antonaci
S
S
Jirillo
E
E
D000368:Aged; D002176:Candida albicans; D003602:Cytotoxicity, Immunologic; D005260:Female; D016178:Granulocyte-Macrophage Colony-Stimulating Factor / Q000494:pharmacology*; D006801:Humans; D007700:Kinetics; D008875:Middle Aged; D009000:Monocytes / Q000187:drug effects* / Q000502:physiology*; D009504:Neutrophils / Q000187:drug effects* / Q000502:physiology*; D010051:Ovarian Neoplasms / Q000097:blood*; D010587:Phagocytosis / Q000187:drug effects / Q000502:physiology; D011994:Recombinant Proteins / Q000494:pharmacology
D011994:Recombinant Proteins; D016178:Granulocyte-Macrophage Colony-Stimulating Factor