title: Epstein-Barr virus in B-cell lymphomas associated with chronic suppurative inflammation.
abstract: Chronic antigenic stimulation is considered to play an important role in neoplastic lymphoid transformation. This paper describes three cases of non-Hodgkin lymphomas (NHLs) associated with long-standing chronic suppuration. Two were primary bone lymphomas associated with long-standing chronic osteomyelitis and one was a primary skin lymphoma associated with chronic venous ulcers with a latent period of 13 years. All were diffuse large B-cell lymphomas, with plasmacytoid differentiation in two cases. Epstein-Barr virus (EBV)-encoded RNAs were demonstrated in virtually all tumour cells in all cases by in situ hybridization. Immunohistochemistry revealed EBV-encoded latent membrane protein (LMP)-1 expression in one case and BZLF1 protein expression in all three cases, whilst the EBV-encoded nuclear antigen (EBNA)-2 was not detected. Kaposi's sarcoma-associated herpesvirus (KSHV) sequences were absent in two cases studied. Our cases show similarities with pyothorax-associated pleural lymphomas reported mainly from Japan and recently from France, which are invariably associated with EBV. These findings suggest a causal effect of EBV in the development of this type of lymphoma complicating long-standing chronic suppuration. Localized immunodepression induced by chronic inflammation or immunosuppressive cytokines may favour the clonal proliferation of EBV-infected B cells.
authors.affiliation: Department of Histopathology, UCL Medical School, London, U.K.
authors.forename: C
authors.initials: C
authors.lastname: Copie-Bergman
authors.forename: G
authors.initials: G
authors.lastname: Niedobitek
authors.forename: D C
authors.initials: DC
authors.lastname: Mangham
authors.forename: J
authors.initials: J
authors.lastname: Selves
authors.forename: K
authors.initials: K
authors.lastname: Baloch
authors.forename: T C
authors.initials: TC
authors.lastname: Diss
authors.forename: D N
authors.initials: DN
authors.lastname: Knowles
authors.forename: G
authors.initials: G
authors.lastname: Delsol
authors.forename: P G
authors.initials: PG
authors.lastname: Isaacson
mesh_terms: D000368:Aged; D000369:Aged, 80 and over; D002908:Chronic Disease; D006566:Herpesviridae Infections / Q000150:complications* / Q000473:pathology; D004854:Herpesvirus 4, Human / Q000235:genetics; D006801:Humans; D007124:Immunoenzyme Techniques; D017403:In Situ Hybridization; D016393:Lymphoma, B-Cell / Q000473:pathology / Q000821:virology*; D008297:Male; D008875:Middle Aged; D010019:Osteomyelitis / Q000150:complications* / Q000473:pathology; D016133:Polymerase Chain Reaction; D012367:RNA, Viral / Q000032:analysis; D014412:Tumor Virus Infections / Q000150:complications* / Q000473:pathology; D014647:Varicose Ulcer / Q000150:complications
chemical_list: D012367:RNA, Viral