title: Altered growth of transgenic tobacco lacking leaf cytosolic pyruvate kinase.
abstract: Previously, we reported that transformation of tobacco (Nicotiana tabacum L.) with a vector containing a potato cytosolic pyruvate kinase (PKc) cDNA generated two plant lines specifically lacking leaf PKc (PKc-) as a result of co-suppression. PKc deficiency in these primary transformants did not appear to alter plant development, although root growth was not examined. Here we report a striking reduction in root growth of homozygous progeny of both PKc- lines throughout development under moderate (600 microE m-2 s-1) or low (100 microE m-2 s-1) light intensities. When both PKc- lines were cultivated under low light, shoot and flower development were also delayed and leaf indentations were apparent. Leaf PK activity in the transformants was significantly decreased at all time points examined, whereas root activities were unaffected. Polypeptides corresponding to PKc were undetectable on immunoblots of PKc- leaf extracts, except in 6-week-old low-light-grown PKc- plants, in which leaf PKc expression appeared to be greatly reduced. The metabolic implications of the kinetic characteristics of partially purified PKc from wild-type tobacco leaves are discussed. Overall, the results suggest that leaf PKc deficiency leads to a perturbation in source-sink relationships.
authors.affiliation: Department of Biology, Queen's University, Kingston, Ontario, Canada.
authors.forename: V L
authors.initials: VL
authors.lastname: Knowles
authors.forename: S G
authors.initials: SG
authors.lastname: McHugh
authors.forename: Z
authors.initials: Z
authors.lastname: Hu
authors.forename: D T
authors.initials: DT
authors.lastname: Dennis
authors.forename: B L
authors.initials: BL
authors.lastname: Miki
authors.forename: W C
authors.initials: WC
authors.lastname: Plaxton
mesh_terms: D003600:Cytosol / Q000201:enzymology; D007700:Kinetics; D008027:Light; D018515:Plant Leaves; D030821:Plants, Genetically Modified; D010947:Plants, Toxic; D011770:Pyruvate Kinase / Q000172:deficiency / Q000235:genetics / Q000378:metabolism*; D014026:Nicotiana / Q000201:enzymology / Q000254:growth & development*
chemical_list: D011770:Pyruvate Kinase