Molecular characterization of type E Clostridium botulinum and comparison to other types of Clostridium botulinum.
Determination of nucleotide sequence upstream to the neurotoxin binding protein (NBP) gene of type E Clostridium botulinum has revealed an open reading frame whose stop codon is only 18 bp apart from the start codon of the NBP gene. Amino acid sequence derived from the corresponding nucleotide sequence suggested the existence of the open reading frame as a 47.8 kDa protein (P-48). Protein data bank search revealed that the 47.8 kDa protein has 80% sequence identity to P-47 of type F C. botulinum. The gene organization of type E. Clostridium botulinum was predicted and compared to other types of C. botulinum. In type E C. botulinum, genes for the P-48, the neurotoxin binding protein and the neurotoxin form an operon which was similar to that of type F C. botulinum. However, type E C. botulinum has a P-18 gene instead of P-21 gene observed in type F C. botulinum, both located upstream to their respective P-48/P-47 gene.
Li
B
B
Department of Biology, University of Massachusetts Dartmouth, 02747, USA.
Qian
X
X
Sarkar
H K
HK
Singh
B R
BR
D000595:Amino Acid Sequence; D001426:Bacterial Proteins; D001483:Base Sequence; D002352:Carrier Proteins / Q000737:chemistry / Q000235:genetics*; D003014:Clostridium botulinum / Q000145:classification / Q000235:genetics*; D005798:Genes, Bacterial / Q000235:genetics*; D047908:Intracellular Signaling Peptides and Proteins; D008969:Molecular Sequence Data; D016366:Open Reading Frames / Q000235:genetics; D009876:Operon / Q000235:genetics; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D013045:Species Specificity
D001426:Bacterial Proteins; D002352:Carrier Proteins; D047908:Intracellular Signaling Peptides and Proteins; C109937:neurotoxin-binding protein, Clostridium