Mapping replication origins, pause sites, and termini by neutral/alkaline two-dimensional gel electrophoresis.
Neutral/alkaline two-dimensional gel electrophoresis is a robust, easily interpretable, sensitive technique that has yielded insights about the in vivo replication of many types of DNA, from multicopy yeast plasmids to single-copy chromosome regions in unsynchronized mammalian cells. It can provide information about directions of replication fork movement and locations of replication origins, termini, and pause sites. Especially when combined with its partner technique, neutral/neutral two-dimensional gel electrophoresis, it is a method of choice for investigation of unknown situations.
Huberman
J A
JA
Department of Molecular and Cellular Biology, Roswell Park Cancer Institute, Buffalo, New York 14263-0001, USA. jhubermn@mcbio.med.buffalo.edu
D000818:Animals; D015180:Electrophoresis, Gel, Two-Dimensional / Q000379:methods*; D006863:Hydrogen-Ion Concentration; D009710:Nucleotide Mapping / Q000379:methods*; D012045:Regulatory Sequences, Nucleic Acid / Q000235:genetics*; D018741:Replication Origin / Q000235:genetics*; D013728:Terminator Regions, Genetic / Q000235:genetics*