Improved determination of urinary cortisol and cortisone, or corticosterone and 11-dehydrocorticosterone by high-performance liquid chromatography with ultraviolet absorbance detection.
A sensitive assay was developed for the determination of low levels of free (unconjugated) glucocorticoids in human, swine (cortisol and cortisone) and rat urine (corticosterone and 11-dehydrocorticosterone), using solid-phase extraction and HPLC with UV absorbance detection (254 nm). Precise quantitation is allowed by the use of internal standards (dexamethasone for swine urine and Reichstein's substance S for rat urine). This simple method allows the use of small urine samples (less than 2 ml), and is suitable for a wide range of applications in human and animal clinical and physiological studies.
Hay
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Laboratoire de Génétique du Stress, INSERM-INRA, Institut François Magendie, Bordeaux, France.
Mormède
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D000818:Animals; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D003345:Corticosterone / Q000031:analogs & derivatives / Q000652:urine; D003348:Cortisone / Q000652:urine; D005260:Female; D006854:Hydrocortisone / Q000652:urine; D006889:Hydroxycorticosteroids / Q000652:urine*; D016014:Linear Models; D008297:Male; D051381:Rats; D011914:Rats, Inbred BN; D011916:Rats, Inbred F344; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013056:Spectrophotometry, Ultraviolet; D013552:Swine
D006889:Hydroxycorticosteroids; C003552:11-dehydrocorticosterone; D003348:Cortisone; D003345:Corticosterone; D006854:Hydrocortisone