Antibodies to long-chain acyl-CoAs. A new tool for lipid biochemistry.
Antibodies directed against long chain acyl-CoAs (having 16 and 18 carbon atoms) have been prepared and are reported for the first time. A modified ELISA procedure adapted to these amphiphilic molecules has been developed: it is a rapid, simple and sensitive test permitting to detect as little as 3 pmol of acyl-CoA. These antibodies represent a new tool for studying long-chain acyl-CoAs. Their use in an immunochemical approach for the study of protein-acyl-CoA interactions is presented.
Maneta-Peyret
L
L
Université Victor Segalen Bordeaux 2, CNRS-UMR 5544, France. lilly.maneta-peyret@biomemb.u-bordeaux2.fr
Sturbois-Balcerzak
B
B
Cassagne
C
C
Moreau
P
P
D000214:Acyl Coenzyme A / Q000032:analysis / Q000737:chemistry / Q000276:immunology*; D051080:Acyl-Carrier Protein S-Malonyltransferase; D000217:Acyltransferases / Q000378:metabolism; D000818:Animals; D000906:Antibodies / Q000276:immunology*; D002462:Cell Membrane / Q000737:chemistry; D003124:Colorimetry; D004797:Enzyme-Linked Immunosorbent Assay; D006056:Golgi Apparatus / Q000737:chemistry / Q000201:enzymology; D007114:Immunization; D015151:Immunoblotting; D019697:Onions; D011817:Rabbits
D000214:Acyl Coenzyme A; D000906:Antibodies; D000217:Acyltransferases; D051080:Acyl-Carrier Protein S-Malonyltransferase