Biocompatibility studies of the single-needle technique in plateletpheresis with the Fresenius AS 104 blood cell separator.
To investigate the biocompatibility of the single-needle technique of the Fresenius AS 104 blood cell separator we analyzed coagulation, complement and hemolysis parameters prior to, during and after five thrombocytaphereses. The data were compared with results of the same parameters from identical donors in the dual-needle procedure. The analysis of bilirubin, lactate dehydrogenase, hydroxybutyrate dehydrogenase and haptoglobin showed no relevant hemolysis. However, changes of the concentrations of coagulation factors XII and XI indicated an activation of coagulation. This was confirmed by an increase of the thrombin-antithrombin III complex during apheresis. No significant change in the complement split product content of the samples obtained during apheresis was found. Complement factors, glucose, lactate, leukocytes, morphology score and thrombocytes were measured in platelet concentrates. The comparison of the platelet products showed no significant differences except for leukocyte contamination. Glucose and lactate concentrations were similar in both procedures. Levels of complement activation markers were not statistically different.
Moog
R
R
Institut für Transfusionsmedizin, Universitätsklinikum Essen, Wendel, Germany.
Kadar
J
J
Müller
N
N
Reiser
M
M
Weisswange
T
T
Ledermann
A E
AE
D001663:Bilirubin / Q000032:analysis; D001672:Biocompatible Materials; D001779:Blood Coagulation Factors / Q000032:analysis; D001782:Blood Donors; D001786:Blood Glucose / Q000032:analysis; D001798:Blood Proteins / Q000032:analysis; D003165:Complement System Proteins / Q000032:analysis; D004798:Enzymes / Q000097:blood; D005260:Female; D006461:Hemolysis; D006801:Humans; D007773:Lactates / Q000097:blood; D009339:Needles; D010983:Plateletpheresis / Q000295:instrumentation* / Q000379:methods*
D001672:Biocompatible Materials; D001779:Blood Coagulation Factors; D001786:Blood Glucose; D001798:Blood Proteins; D004798:Enzymes; D007773:Lactates; D003165:Complement System Proteins; D001663:Bilirubin