title: ISRf1, a transposable insertion sequence from Sinorhizobium fredii.
abstract: Sinorhizobium fredii strain HH103, a nitrogen-fixing bacterial symbiont of plants, contains an insertion sequence (IS) that can transpose into plasmid pMUS248 and activate a promoterless TcR gene that is normally not expressed. We have cloned and characterized this element, which we designate ISRf1. The IS is 1002 bp in length, contains a single 513-bp open reading frame (ORF), is flanked by imperfect 36-bp terminal inverted repeats, and creates 5-bp target duplications. Two copies of ISRf1 are present in the genome of HH103, but it is absent from 12 other Sinorhizobium and Rhizobium strains. The element transposes at a frequency of 2.7 x 10(-6) per generation per cell.
authors.affiliation: Departamento de Microbiología, Facultad de Biología , Universidad de Sevilla, Spain.
authors.forename: J M
authors.initials: JM
authors.lastname: Vinardell
authors.forename: F J
authors.initials: FJ
authors.lastname: Ollero
authors.forename: H B
authors.initials: HB
authors.lastname: Krishnan
authors.forename: M
authors.initials: M
authors.lastname: del Rosario Espuny
authors.forename: E
authors.initials: E
authors.lastname: Villalobo
authors.forename: S G
authors.initials: SG
authors.lastname: Pueppke
authors.forename: J E
authors.initials: JE
authors.lastname: Ruiz-Sainz
mesh_terms: D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000235:genetics*; D001483:Base Sequence; D004251:DNA Transposable Elements / Q000235:genetics*; D004269:DNA, Bacterial; D004926:Escherichia coli / Q000378:metabolism; D015870:Gene Expression; D005798:Genes, Bacterial; D008969:Molecular Sequence Data; D012230:Rhizobiaceae / Q000235:genetics*
chemical_list: D001426:Bacterial Proteins; D004251:DNA Transposable Elements; D004269:DNA, Bacterial; C110084:ISRf1 protein, Sinorhizobium fredii