Effects of enprostil on gastric endocrine secretion during chronic administration of lansoprazole.
We investigated changes in the secretory kinetics of gastric endocrine cells related to the administration of lansoprazole, and the effects of enprostil on these altered kinetics. Male Wistar-derived 8-week-old rats were allotted to a control group, a lansoprazole administration group, an enprostil administration group, and a lansoprazole + enprostil administration group. Lansoprazole (30 mg/kg once a day for 4 weeks) and enprostil (10 micrograms/kg twice a day for 4 weeks) were administered into the gastric lumen with a gastric tube. At this time, blood was collected and immunohistological staining of gastric endocrine cells was conducted to investigate the secretory kinetics. Lansoprazole administration induced hypergastrinemia, increase of gastrin cells, and increase of enterochromaffin-like cells. Enprostil administration induced increase of somatostatin cells. The group administered lansoprazole + enprostil exhibited significant decreases in serum gastrin level, total gastrin cell count, and total enterochromaffin-like cell count, compared with the group administered lansoprazole alone. These findings suggest that enprostil may ameliorate the alteration in gastric endocrine secretion produced by the chronic administration of lansoprazole.
Omura
N
N
Department of Surgery (II), Jikei University School of Medicine, Tokyo, Japan.
Kashiwagi
H
H
Aoki
T
T
Omura
K
K
Fukuchi
Y
Y
D053799:2-Pyridinylmethylsulfinylbenzimidazoles; D000818:Animals; D000897:Anti-Ulcer Agents / Q000494:pharmacology*; D004347:Drug Interactions; D016620:Enprostil / Q000008:administration & dosage / Q000494:pharmacology*; D004759:Enterochromaffin Cells / Q000166:cytology / Q000187:drug effects; D005753:Gastric Mucosa / Q000187:drug effects / Q000378:metabolism; D019863:Gastrin-Secreting Cells / Q000166:cytology / Q000187:drug effects; D005755:Gastrins / Q000097:blood; D007150:Immunohistochemistry; D064747:Lansoprazole; D008297:Male; D009853:Omeprazole / Q000008:administration & dosage / Q000031:analogs & derivatives* / Q000494:pharmacology; D051381:Rats; D017208:Rats, Wistar; D013004:Somatostatin / Q000097:blood; D019864:Somatostatin-Secreting Cells / Q000166:cytology / Q000187:drug effects; D013270:Stomach / Q000166:cytology* / Q000187:drug effects
D053799:2-Pyridinylmethylsulfinylbenzimidazoles; D000897:Anti-Ulcer Agents; D005755:Gastrins; D064747:Lansoprazole; D013004:Somatostatin; D016620:Enprostil; D009853:Omeprazole