Comparative analysis of equine lymphocyte subsets in whole blood and gradient-purified samples.
In the present study, two methods of lymphocyte preparation, whole blood lysis and Ficoll-Paque separation, prior to FACS analysis were compared. The comparison was done with single and dual-colour staining techniques. Monoclonal antibodies (mAb) against eCD4, eCD5, eCD8 and eMHC class II were used. There was no significant difference in the results obtained by these two methods.
Akens
M K
MK
Department of Veterinary Internal Medicine, University of Zurich, Switzerland. makens@vetklinik.unizh.ch
Holznagel
E
E
Franchini
M
M
Bracher
V
V
D000818:Animals; D005260:Female; D005434:Flow Cytometry; D006736:Horses / Q000276:immunology*; D016130:Immunophenotyping; D016131:Lymphocyte Subsets / Q000276:immunology*; D008297:Male