Mutational analysis of the potato virus Y 5' untranslated region for alteration in translational enhancement in tobacco protoplasts.
The 185 nucleotide 5' untranslated region (5'UTR) of potato virus Y ordinary strain (PVY-O) showed translation-enhancing activity on the beta-glucuronidase (GUS) gene in tobacco protoplasts. Mutational analysis of the 5'UTR was done to find sequence motifs necessary for the enhancement. Deletions within the 1-130 nucleotide region of 5'UTR stimulated the GUS expression in some cases, while the GUS activity declined with deletions in the 131-185 nucleotide region. The results indicated that the last 55 nucleotides of PVY-O 5'UTR might play the much important role in the translational enhancement in plant cells.
Yang
L J
LJ
Department of Biotechnology, Graduate School of Agricultural and Life Sciences, University of Tokyo, Japan.
Hidaka
M
M
Sonoda
J
J
Masaki
H
H
Uozumi
T
T
D000595:Amino Acid Sequence; D001483:Base Sequence; D015967:Gene Expression Regulation, Viral; D005966:Glucuronidase / Q000235:genetics*; D008969:Molecular Sequence Data; D009154:Mutation; D010947:Plants, Toxic; D017800:Potyvirus / Q000201:enzymology / Q000235:genetics*; D014176:Protein Biosynthesis; D011523:Protoplasts / Q000378:metabolism; D016415:Sequence Alignment; D014026:Nicotiana / Q000235:genetics
D005966:Glucuronidase