[Immunohistochemical studies on organ tropism of different biotypes of BVD virus in experimentally infected sheep fetuses].
Paraffin sections from various organs of sheep fetuses following transplacental infection with non-cytopathogenic (ncp) bovine viral diarrhoea virus (BVDV) or cytopathogenic (cp) BVDV were stained immunohistochemically with BVDV-specific monoclonal antibodies. Comparison of the distribution of viral antigen in sections from fetuses of experiment A revealed that in organs such as parotid, thyroid, thymus, lung, spleen, kidney, liver and skin from 20 days post inoculation (p.i.) onwards numerous antigen-containing cells were present. In organs of fetuses infected with cp BVDV, however, antigen-positive cells were only detectable until days 10 and 14 p.i. These findings suggest that the ncp BVDV used in experiment A replicated considerably faster and more efficient than the cp BVDV used in experiment B and that the two virus biotypes differ considerably concerning their tropism for fetal ovine organs.
Hewicker-Trautwein
M
M
Institut für Pathologie, Tierärztliche Hochschule Hannover.
Trautwein
G
G
Frey
H R
HR
Liess
B
B
D000818:Animals; D000956:Antigens, Viral / Q000032:analysis; D001912:Bovine Virus Diarrhea-Mucosal Disease / Q000196:embryology*; D002417:Cattle; D005260:Female; D005333:Fetus / Q000821:virology*; D007150:Immunohistochemistry / Q000379:methods; D009928:Organ Specificity; D010576:Pestivirus / Q000145:classification* / Q000302:isolation & purification / Q000502:physiology; D011247:Pregnancy; D012756:Sheep; D014779:Virus Replication
D000956:Antigens, Viral