Mapping replication origins by neutral/neutral two-dimensional gel electrophoresis.
Neutral/neutral two-dimensional gel electrophoresis is a sensitive physical mapping technique that has been successfully used to unambiguously identify replication initiation sites in genomes of widely varying complexity in vivo. The technique exploits the fact that restriction fragments containing different classes of replicative intermediates (single forks, initiation bubbles, or termination structures) migrate to different and characteristic positions in agarose gels. The replication pattern of any region of interest can then be determined by sequential hybridization with appropriate radioactive probes from that region.
Dijkwel
P A
PA
Department of Biochemistry, University of Virginia School of Medicine, Charlottesville 22908, USA. pad9u@virginia.edu
Hamlin
J L
JL
D000818:Animals; D002874:Chromosome Mapping / Q000379:methods*; D015180:Electrophoresis, Gel, Two-Dimensional / Q000379:methods*; D018741:Replication Origin / Q000235:genetics*