title: Monoamine metabolite levels in CSF of SIV-infected rhesus monkeys (Macaca mulatta).
abstract: Increasing evidence suggests the involvement of the basal ganglia in HIV-1-infected patients. We used SIV-macaques, an animal model of HIV-1 infection, to investigate changes in CSF biogenic amine metabolites over time and compared them with control animals. The dopamine and serotonin metabolites, 3,4-dihydroxyphenylacetic acid (DOPAC), homovanillic acid (HVA) and 5-hydroxyindoleacetic acid (5-HIAA), were analysed by reverse-phase chromatography with electrochemical detection. DOPAC concentrations were significantly increased in SIV-infected animals compared with controls. 5-HIAA and HVA remained unchanged. However, the longitudinal assessment of metabolites up to 3 months post-infection revealed a significant increase in 5-HIAA. Our results reflect the effects of SIV early stage infection on monoamine systems in brain and further validate the simian model for AIDS research.
authors.affiliation: Department of Psychiatry, Clinical Neurochemistry, Julius-Maximilians-University, Würzburg, Germany.
authors.forename: E
authors.initials: E
authors.lastname: Koutsilieri
authors.forename: M E
authors.initials: ME
authors.lastname: Götz
authors.forename: S
authors.initials: S
authors.lastname: Sopper
authors.forename: C
authors.initials: C
authors.lastname: Stahl-Hennig
authors.forename: M
authors.initials: M
authors.lastname: Czub
authors.forename: V
authors.initials: V
authors.lastname: ter Meulen
authors.forename: P
authors.initials: P
authors.lastname: Riederer
mesh_terms: D015102:3,4-Dihydroxyphenylacetic Acid / Q000134:cerebrospinal fluid; D000163:Acquired Immunodeficiency Syndrome / Q000134:cerebrospinal fluid*; D000818:Animals; D001679:Biogenic Amines / Q000134:cerebrospinal fluid*; D004298:Dopamine / Q000134:cerebrospinal fluid; D004563:Electrochemistry / Q000379:methods; D015497:HIV-1; D006719:Homovanillic Acid / Q000134:cerebrospinal fluid; D006801:Humans; D006897:Hydroxyindoleacetic Acid / Q000134:cerebrospinal fluid; D008253:Macaca mulatta; D012016:Reference Values; D012701:Serotonin / Q000134:cerebrospinal fluid; D015302:Simian Immunodeficiency Virus; D013997:Time Factors
chemical_list: D001679:Biogenic Amines; D015102:3,4-Dihydroxyphenylacetic Acid; D012701:Serotonin; D006897:Hydroxyindoleacetic Acid; D004298:Dopamine; D006719:Homovanillic Acid