title: Cloning and nucleotide sequence of the DNA gyrase gyrA gene from Serratia marcescens and characterization of mutations in gyrA of quinolone-resistant clinical isolates.
abstract: The sequence of the DNA gyrase gyrA gene of Serratia marcescens ATCC 14756 was determined. An open reading frame of 2,640 nucleotides coding for a polypeptide with a calculated molecular mass of 97,460 was found, and its sequence complemented the sequence of an Escherichia coli gyrA temperature-sensitive mutation. Analysis of the PCR products of the quinolone resistance-determining regions of gyrA genes from six quinolone-resistant clinical isolates revealed a single amino acid substitution, Ser-83 to Arg or Asp-87 to Tyr, in all six mutants, suggesting that a mutational alteration in gyrA is a common mechanism of quinolone resistance in S. marcescens.
authors.affiliation: Department of Pharmacology and Molecular Biology, C&C Research Laboratories, Kyunggi-do, Korea.
authors.forename: J H
authors.initials: JH
authors.lastname: Kim
authors.forename: E H
authors.initials: EH
authors.lastname: Cho
authors.forename: K S
authors.initials: KS
authors.lastname: Kim
authors.forename: H Y
authors.initials: HY
authors.lastname: Kim
authors.forename: Y M
authors.initials: YM
authors.lastname: Kim
mesh_terms: D042462:4-Quinolones; D000595:Amino Acid Sequence; D000890:Anti-Infective Agents / Q000627:therapeutic use; D001483:Base Sequence; D003001:Cloning, Molecular; D027081:DNA Gyrase; D004250:DNA Topoisomerases, Type II / Q000235:genetics*; D004352:Drug Resistance, Microbial / Q000235:genetics; D006801:Humans; D008969:Molecular Sequence Data; D017354:Point Mutation; D016868:Serratia Infections / Q000188:drug therapy / Q000201:enzymology / Q000235:genetics*; D012706:Serratia marcescens / Q000187:drug effects / Q000201:enzymology / Q000235:genetics*; D059005:Topoisomerase II Inhibitors
chemical_list: D042462:4-Quinolones; D000890:Anti-Infective Agents; D059005:Topoisomerase II Inhibitors; D027081:DNA Gyrase; D004250:DNA Topoisomerases, Type II