The influence of oral glucose intake on binding and degradation of 125I-insulin by receptors on erythrocytes as well as on insulin and C-peptide serum levels in patients after myocardial infarction and healthy individuals.
In this study, we investigated the influence of glucose administration on binding and degradation of 125I-insulin by receptors on erythrocytes as well as on insulin and C-peptide serum levels in 15 patients after myocardial infarction and in 15 age-matched healthy persons. Venous blood samples were taken directly before and at 30, 60 and 120 minutes after oral administration of 75 g of glucose. In the collected blood samples serum glucose, insulin and C-peptide levels were determined. Binding and degradation of 125I-insulin by specific receptors on red blood cells were evaluated using the method described by Gambhir and modified by the authors. Serum insulin and C-peptide levels were significantly higher while binding of 125I-insulin to erythrocytes was decreased in patients after myocardial infarction. These results seem to support the hypothesis that insulin resistance and hyperinsulinism play a role in the pathogenesis of ischaemic heart disease. Impaired degradation of 125I-insulin during the oral glucose tolerance test in the patients after myocardial infarction indicates that insulin resistance is located at the receptor level.
Rychlewski
T
T
Chair of Physiology, Biochemistry and Hygiene, University School of Physical Education, Poznań, Poland.
Szcześniak
L
L
Dylewicz
P
P
Deskur
E
E
Przywarska
I
I
Kasprzak
Z
Z
Karolkiewicz
J
J
Konys
L
L
D000328:Adult; D002096:C-Peptide / Q000097:blood*; D004912:Erythrocytes / Q000378:metabolism*; D005947:Glucose / Q000494:pharmacology*; D005951:Glucose Tolerance Test; D006801:Humans; D007328:Insulin / Q000097:blood*; D007457:Iodine Radioisotopes; D008875:Middle Aged; D009203:Myocardial Infarction / Q000097:blood*
D002096:C-Peptide; D007328:Insulin; D007457:Iodine Radioisotopes; D005947:Glucose