Suppression of cell growth by heavy chain ferritin.
While producing recombinant rat liver H and L chain ferritin homopolymers using the baculovirus expression system, we noticed that rat liver H chain ferritin, but not L chain ferritin, had a suppressive effect on the growth of Spodoptera frugiperda (Sf-21) cells. Suppression was observed immediately after infection with recombinant H chain ferritin baculovirus prepared from lysed infected cells. Immediate suppression was observed when purified with either recombinant H chain apoferritin or various holoferritins (loaded with 1,970 +/- 50 or 2,520 +/- 90 atoms of iron/ferritin) indicating that suppression was not due to sequestration of iron required for cell growth. Suppression by H chain ferritin was also observed upon attempting to express the protein in Escherichia coli. Strategies for expression of recombinant rat liver H and L ferritin homopolymers in both prokaryotic and eukaryotic expression systems were developed.
Guo
J H
JH
Biotechnology Center, Utah State University, Logan 84322-4705, USA.
Juan
S H
SH
Aust
S D
SD
D000818:Animals; D016367:Baculoviridae / Q000235:genetics; D002455:Cell Division / Q000187:drug effects; D004926:Escherichia coli / Q000166:cytology / Q000187:drug effects / Q000235:genetics; D005293:Ferritins / Q000096:biosynthesis* / Q000235:genetics / Q000494:pharmacology; D051381:Rats; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000494:pharmacology; D018411:Spodoptera / Q000166:cytology / Q000187:drug effects / Q000235:genetics; D014170:Transformation, Genetic
D011994:Recombinant Proteins; D005293:Ferritins