Microassay for determination of itraconazole and hydroxyitraconazole in plasma and tissue biopsies.
A simple, rapid and sensitive method for the extraction and HPLC analysis of itraconazole and hydroxyitraconazole in tissue and plasma or serum is described. Tissue (5-100 mg) and plasma (0.1 ml) underwent a simple extraction into methanol. Chromatography was performed on a Novapak C18 column using a mobile phase of water-acetonitrile-diethylamine (42:58:0.05, v/v), pH 2.45, with a flow-rate of 1.5 ml/min. Fluorescence was measured at excitation 260 nm and emission 365 nm. The procedure produced a linear curve for the concentration range 10-1000 ng/ml. The development of the assay produced accurate, rapid repeatable results for both tissue and plasma or serum.
Cox
S K
SK
Department of Comparative Medicine, College of Veterinary Medicine, University of Tennessee, Knoxville 37901, USA.
Orosz
S
S
Burnette
J
J
Frazier
D
D
D000818:Animals; D000935:Antifungal Agents / Q000008:administration & dosage / Q000032:analysis* / Q000378:metabolism; D001706:Biopsy; D001717:Birds; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D002940:Circadian Rhythm; D017964:Itraconazole / Q000008:administration & dosage / Q000032:analysis* / Q000378:metabolism; D008099:Liver / Q000737:chemistry; D009994:Osmolar Concentration; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
D000935:Antifungal Agents; D017964:Itraconazole