A repetitive DNA sequence common to the different B chromosomes of the genus Brachycome.
Dot-like micro B chromosomes of Brachycome dichromosomatica were analysed for their sequence composition. Southern hybridization patterns of a total micro B probe to genomic DNA from plants with and without micro Bs demonstrated that the micro Bs shared sequences with the A chromosomes. In addition to telomere, rDNA and common A and B chromosome sequences, a new B-specific, highly methylated tandem repeat (Bdm29) was detected. After in situ hybridization with Bdm29 the entire micro B chromosome was labelled and clustering of the condensed micro Bs could be observed at interphase. A high number of Bdm29-like sequences were also found in the larger B chromosomes of B. dichromosomatica and in other Bs within the genus Brachycome.
Houben
A
A
Department of Genetics, The University of Adelaide, SA 5005, Australia.
Leach
C R
CR
Verlin
D
D
Rofe
R
R
Timmis
J N
JN
D001483:Base Sequence; D002875:Chromosomes / Q000737:chemistry / Q000235:genetics*; D018744:DNA, Plant / Q000032:analysis; D008969:Molecular Sequence Data; D018515:Plant Leaves / Q000737:chemistry / Q000235:genetics; D010944:Plants / Q000235:genetics*; D012091:Repetitive Sequences, Nucleic Acid / Q000235:genetics*; D016615:Telomere / Q000235:genetics
D018744:DNA, Plant