Direct sequencing of unpurified PCR-amplified DNA by semi-exponential cycle sequencing (SECS).
A simple technique for direct sequencing of PCR-amplified templates without purification of the PCR reaction product is presented. This method does not require an additional synthesis step after template amplification, and can generate sequence information form as little as 0.1 fmol of unpurified template.
Sarkar
G
G
Mayo Clinic, Rochester, MN 55905, USA. sarkar.gobinda@mayo.edu
Bolander
M E
ME
D004247:DNA / Q000032:analysis* / Q000302:isolation & purification; D016133:Polymerase Chain Reaction / Q000379:methods*; D017422:Sequence Analysis, DNA; D013698:Templates, Genetic
D004247:DNA