title: Human tissue factor pathway inhibitor fused to CD4 binds both FXa and TF/FVIIa at the cell surface.
abstract: Tissue factor pathway inhibitor (TFPI) is one of the main regulators of the tissue factor (TF) pathway of coagulation. To tether human TFPI to the cell surface, full length or truncated TFPI lacking the third Kunitz domain were fused with domains three and four and the carboxy-terminal sequence of human CD4. Constructs were transfected into a mouse fibroblast cell line and individual clones were checked for expression using monoclonal antibodies directed against the first two TFPI Kunitz domains and against CD4. Specific human FXa binding was detected by flow cytometry using an anti-FX polyclonal antibody, and inhibition of FXa proteolytic activity was verified by chromogenic substrate assay using S-2765. In addition, TFPI-CD4-expressing cells, preincubated with FXa, specifically bound human TF-FVIIa complexes as revealed with an anti-human TF polyclonal antibody. No functional difference was observed between full length or truncated TFPI-CD4. These results demonstrate that functionally intact TFPI can be tethered to the cell surface. Genetic manipulation of, for example, endothelial cells leading to the stable expression of TFPI may inhibit the development of coronary artery heart disease following cardiac allotransplantation, and may inhibit thrombosis in the context of xenotransplantation.
authors.affiliation: Dept. of Immunology, MRC Clinical Sciences Centre, London, UK. kristian.riesbeck@swipnet.se
authors.forename: K
authors.initials: K
authors.lastname: Riesbeck
authors.forename: A
authors.initials: A
authors.lastname: Dorling
authors.forename: G
authors.initials: G
authors.lastname: Kemball-Cook
authors.forename: J H
authors.initials: JH
authors.lastname: McVey
authors.forename: M
authors.initials: M
authors.lastname: Jones
authors.forename: E G
authors.initials: EG
authors.lastname: Tuddenham
authors.forename: R I
authors.initials: RI
authors.lastname: Lechler
mesh_terms: D000818:Animals; D015704:CD4 Antigens / Q000235:genetics* / Q000378:metabolism*; D002460:Cell Line; D002462:Cell Membrane / Q000378:metabolism*; D015942:Factor VIIa / Q000378:metabolism*; D015951:Factor Xa / Q000378:metabolism*; D015870:Gene Expression / Q000235:genetics; D006801:Humans; D008074:Lipoproteins / Q000235:genetics* / Q000378:metabolism*; D051379:Mice; D010446:Peptide Fragments / Q000235:genetics / Q000502:physiology; D011485:Protein Binding; D017434:Protein Structure, Tertiary; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000378:metabolism; D012680:Sensitivity and Specificity; D013925:Thromboplastin / Q000378:metabolism*
chemical_list: D015704:CD4 Antigens; D008074:Lipoproteins; D010446:Peptide Fragments; D011993:Recombinant Fusion Proteins; C051928:lipoprotein-associated coagulation inhibitor; D013925:Thromboplastin; D015942:Factor VIIa; D015951:Factor Xa