title: Electropolymerization of a phenol-modified peptide for use in receptor-ligand interactions studied by surface plasmon resonance.
abstract: The combination of surface plasmon resonance (SPR) with an electrochemical method for surface modification is presented. The SLP1 sequence of the sodium channel protein of rat cardiac muscle cells was N-terminally modified with an electropolymerizable group and immobilized on a gold-coated glass slide by oxidative polymerization. The resulting peptide-functionalized substrate was incubated with a polyclonal-specific anti-SLP1 serum. Growth of the peptide layer and the immunological reaction between ligand and receptor were detected on-line by SPR. The applicability of this approach for the rapid and selective analysis of receptor-ligand interactions is demonstrated.
authors.affiliation: Institut für Organische Chemie, Universität Tübingen, Germany.
authors.forename: S
authors.initials: S
authors.lastname: Kienle
authors.forename: S
authors.initials: S
authors.lastname: Lingler
authors.forename: W
authors.initials: W
authors.lastname: Kraas
authors.forename: A
authors.initials: A
authors.lastname: Offenhäusser
authors.forename: W
authors.initials: W
authors.lastname: Knoll
authors.forename: G
authors.initials: G
authors.lastname: Jung
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D015374:Biosensing Techniques; D008024:Ligands; D008969:Molecular Sequence Data; D009206:Myocardium / Q000378:metabolism*; D010455:Peptides; D019800:Phenol; D051381:Rats; D011956:Receptors, Cell Surface / Q000032:analysis* / Q000378:metabolism; D015222:Sodium Channels / Q000032:analysis* / Q000378:metabolism
chemical_list: D008024:Ligands; D010455:Peptides; D011956:Receptors, Cell Surface; D015222:Sodium Channels; D019800:Phenol