title: Tissue uptake and interconversion of plasma unesterified 14C linoleic acid in the guinea pig.
abstract: Part of the arachidonic acid (20:4, n - 6) pools in the gastrointestinal tract and blood forming tissues may be formed by local interconversion of linoleic acid (18:2, n - 6) taken up as a free fatty acid from blood. This study examined the rate of uptake and interconversion of unesterified 14C-18:2 by different tissues in young guinea pigs. The clearance rate of 14C-18:2 was fast, and the initial half-life was 6.3 s. The retention of 14C in tissue lipids was 1.6-1.8% g-1 in the liver, 0.4% g-1 in stomach, 0.7% g-1 in small intestine, 0.2% g-1 in colon, 0.4% g-1 in bone marrow and 0.7% g-1 in spleen. Autoradiographic localization of 3H-18:2 under light microscope demonstrated that most of the 3H radioactivity of the gastrointestinal tract was in the mucosa, in both villus and crypt cells. In bone marrow smears, a high density of silver grains was found in megakaryocytes. The percent of 14C in delta6 desaturase products was higher in gastrointestinal tract, heart, lung, bone marrow and spleen than in liver. The ratio of 14C-20:3/14C-20:4 formation in most tissues was high, and a notable finding being a lower rate of 20:4 formation from plasma free 18:2 in the liver, (170 pmol min-1) than in the gastrointestinal tract (428 pmol min-1) and bone marrow (1203 pmol min-1). The local interconversion of 18:2 into delta6 desaturase products is thus an important source of 20:4 in these organs in guinea pigs.
authors.affiliation: Cell Biology Department 1, University Hospital, Lund, Sweden.
authors.forename: L
authors.initials: L
authors.lastname: Zhou
authors.forename: N
authors.initials: N
authors.lastname: Xu
authors.forename: A
authors.initials: A
authors.lastname: Nilsson
mesh_terms: D000818:Animals; D016718:Arachidonic Acid / Q000378:metabolism; D001345:Autoradiography; D001853:Bone Marrow / Q000378:metabolism; D002250:Carbon Radioisotopes / Q000378:metabolism; D004064:Digestive System / Q000378:metabolism; D044943:Fatty Acid Desaturases / Q000378:metabolism; D005230:Fatty Acids, Nonesterified / Q000378:metabolism* / Q000493:pharmacokinetics; D006168:Guinea Pigs; D006651:Histocytochemistry; D019787:Linoleic Acid / Q000097:blood / Q000378:metabolism* / Q000493:pharmacokinetics; D050602:Linoleoyl-CoA Desaturase; D008099:Liver / Q000378:metabolism; D008168:Lung / Q000378:metabolism; D008533:Megakaryocytes / Q000378:metabolism; D008657:Metabolic Clearance Rate; D009206:Myocardium / Q000378:metabolism; D012044:Regression Analysis; D013154:Spleen / Q000378:metabolism
chemical_list: D002250:Carbon Radioisotopes; D005230:Fatty Acids, Nonesterified; D016718:Arachidonic Acid; D019787:Linoleic Acid; D044943:Fatty Acid Desaturases; D050602:Linoleoyl-CoA Desaturase