title: Automated bone marrow concentration using the Fresenius AS 104 blood cell separator.
abstract: We concentrated freshly taken bone marrow (BM) pooled from 21 patients from an original 1.265 ml (+/- 537 ml) down to 128 ml (+/- 36 ml) within 40-70 min with a modified version of the 'grancollect protocol' on the Fresenius AS 104 blood-cell separator using the P1-Y set. An average of 47% (+/- 21%) fo the initially present mononuclear cells and 68% (+/- 47%) of the colony-forming cells could be obtained in the concentrate. The erythrocyte concentration was reduced to 7% (+/- 4%) of the original amount. The technique described is very effective and makes is possible to obtain a BM cell population that is suitable for both immunomagnetic purging and cryopreservation or transplantation, e.g. in the case of blood group incompatibility.
authors.affiliation: Abteilung für Transfusionsmedizin, Friedrich-Alexander-Universität Erlangen.
authors.forename: J
authors.initials: J
authors.lastname: Zingsem
authors.forename: V
authors.initials: V
authors.lastname: Weisbach
authors.forename: R
authors.initials: R
authors.lastname: Zimmermann
authors.forename: T
authors.initials: T
authors.lastname: Zeiler
authors.forename: H
authors.initials: H
authors.lastname: Mitschulat
authors.forename: J
authors.initials: J
authors.lastname: Beyer
authors.forename: R
authors.initials: R
authors.lastname: Eckstein
mesh_terms: D001331:Automation / Q000295:instrumentation / Q000379:methods; D001854:Bone Marrow Cells; D016465:Bone Marrow Purging / Q000379:methods; D002469:Cell Separation / Q000295:instrumentation* / Q000379:methods; D015925:Cryopreservation / Q000379:methods; D004867:Equipment Design; D006098:Granulocytes; D006801:Humans; D018189:Immunomagnetic Separation; D009373:Neoplasms, Germ Cell and Embryonal / Q000473:pathology / Q000628:therapy*; D054198:Precursor Cell Lymphoblastic Leukemia-Lymphoma / Q000473:pathology