Production, metabolism and effect of platelet-activating factor on the growth of the human K562 erythroid cell line.
The human immature K562 erythroid cell line was studied for its capacity to produce and to metabolize the phospholipid molecule platelet-activating factor (PAF). K562 cells produced PAF under calcium ionophore stimulation. Lyso PAF and acetyl-CoA (the acetate donor molecule for the acetylation of lyso PAF into PAF) had no effect on the amounts of PAF produced by ionophore-stimulated cells. The metabolism of PAF and lyso PAF by K562 cells was compared to that of freshly-isolated human bone marrow erythroblasts and blood erythrocytes. K562 cells rapidly metabolized [3H]PAF and [3H]lyso PAF with 1-alkyl analogue of phosphatidylcholine as the major metabolic product. In contrast, blood erythrocytes did not. PAF acetylhydrolase activity levels in K562 cells and bone marrow erythroblasts were similar and higher than in blood erythrocytes. PAF (1-100 nM) stimulated [3H]thymidine incorporation in K562 cells grown in low serum concentration, a non-metabolizable PAF agonist being more potent than PAF to stimulate thymidine incorporation. PAF receptor mRNA was detected in K562 cells by polymerase chain reaction on reverse transcripts. The present study demonstrates that K562 cells produce and metabolize PAF and underlines the putative role of erythroid precursors in the modulation of bone marrow PAF concentrations. The effect of PAF on the growth of K562 cells might be mediated through PAF receptors suggesting a potential role of PAF on the proliferation and functions of human erythroid marrow precursors.
Dupuis
F
F
Laboratoire d'Hématologie Expérimentale, Faculté de Médecine, Limoges, France.
Levasseur
S
S
Jean-Louis
F
F
Dulery
C
C
Praloran
V
V
Denizot
Y
Y
Michel
L
L
D043203:1-Alkyl-2-acetylglycerophosphocholine Esterase; D000105:Acetyl Coenzyme A / Q000494:pharmacology; D000001:Calcimycin / Q000494:pharmacology; D002455:Cell Division; D002478:Cells, Cultured; D004900:Erythroblasts; D004912:Erythrocytes / Q000166:cytology / Q000201:enzymology / Q000378:metabolism*; D006801:Humans; D007476:Ionophores / Q000494:pharmacology; D004915:Leukemia, Erythroblastic, Acute; D010741:Phospholipases A / Q000378:metabolism; D010972:Platelet Activating Factor / Q000031:analogs & derivatives / Q000096:biosynthesis / Q000378:metabolism* / Q000494:pharmacology*; D010980:Platelet Membrane Glycoproteins / Q000235:genetics; D012333:RNA, Messenger / Q000032:analysis; D011956:Receptors, Cell Surface; D043562:Receptors, G-Protein-Coupled; D014407:Tumor Cells, Cultured
D007476:Ionophores; C029271:O-deacetyl platelet activating factor; D010972:Platelet Activating Factor; D010980:Platelet Membrane Glycoproteins; D012333:RNA, Messenger; D011956:Receptors, Cell Surface; D043562:Receptors, G-Protein-Coupled; C039351:platelet activating factor receptor; D000001:Calcimycin; D000105:Acetyl Coenzyme A; D010741:Phospholipases A; D043203:1-Alkyl-2-acetylglycerophosphocholine Esterase