In vitro isolation and cultivation of Cowdria ruminantium under serum-free culture conditions.
Three stocks of Cowdria ruminantium, the causative agent of heartwater in domestic ruminants, were propagated in bovine endothelial cells in a serum-free culture medium. The Vosloo, Welgevonden and Senegal stocks were propagated for a period of more than 203, 134, and 43 days, respectively. Two of the C ruminantium stocks (Vosloo and Senegal) were also successfully initiated under serum-free culture conditions. The serum-free medium consisted of a modified HL-1 medium. The Senegal stock was successfully propagated in Dulbecco's modified Eagle's medium nutrient mixture Ham F-12 containing 10 per cent fetal bovine serum.
Zweygarth
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Onderstepoort Veterinary Institute, Protozoology Division, South Africa.
Vogel
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Josemans
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Horn
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D000818:Animals; D002417:Cattle; D002418:Cattle Diseases / Q000382:microbiology / Q000473:pathology; D002460:Cell Line; D016895:Culture Media, Serum-Free; D016995:Ehrlichia ruminantium / Q000254:growth & development* / Q000302:isolation & purification*; D004730:Endothelium, Vascular / Q000166:cytology / Q000382:microbiology; D015511:Goat Diseases / Q000382:microbiology / Q000473:pathology; D006041:Goats; D006357:Heartwater Disease / Q000382:microbiology / Q000473:pathology; D012756:Sheep; D012757:Sheep Diseases / Q000382:microbiology / Q000473:pathology; D013997:Time Factors
D016895:Culture Media, Serum-Free