[AccBSI - a novel restriction endonuclease from Acinetobacter calcoaceticus BS].
The recognition site of a new restriction endonuclease from Acinetobacter calcoaceticus BS was determined. This is a nonpalindromic sequence. AccBSI restrictase cleaves DNA chains in the middle of the recognition sequence; therefore, ligation of its digestion fragments restored AccBSI recognition sites and generated palindromic sequences recognized by SacI and SacII restrictases.
Abdurashitov
M A
MA
Kiseleva
E V
EV
Miakisheva
T V
TV
Dedkov
V S
VS
Shevchenko
A V
AV
Degtiarev
C Kh
CKh
D016954:Acinetobacter calcoaceticus / Q000201:enzymology* / Q000235:genetics; D001665:Binding Sites / Q000235:genetics; D004262:DNA Restriction Enzymes / Q000235:genetics / Q000302:isolation & purification*; D004269:DNA, Bacterial / Q000235:genetics / Q000378:metabolism; D015252:Deoxyribonucleases, Type II Site-Specific / Q000235:genetics / Q000302:isolation & purification*; D006868:Hydrolysis; D013379:Substrate Specificity
D004269:DNA, Bacterial; D004262:DNA Restriction Enzymes; C109672:CCGCTC-specific type II deoxyribonucleases; D015252:Deoxyribonucleases, Type II Site-Specific