title: Molecular cloning of DNAs encoding the regulatory subunits of elongin from Saccharomyces cerevisiae and Drosophila melanogaster.
abstract: The Elongin complex strongly stimulates the rate of elongation by RNA polymerase II by suppressing transient pausing by polymerase at many sites along the DNA. Elongin is composed of a transcriptionally active A subunit and two positive regulatory B and C subunits. The Elongin complex is a potential target for regulation by the von Hippel-Lindau (VHL) tumor suppressor protein, which is capable of binding stably to the Elongin BC complex and preventing it from activating Elongin A. Here, we report the molecular cloning of a Saccharomyces cerevisiae genomic DNA encoding Elongin C subunit and of Drosophila cDNAs encoding Elongin B and C subunits. The predicted amino acid sequence of each protein shows a high degree of similarity with the mammalian proteins. The recombinant yeast Elongin C protein interacts with both mammalian Elongin A and VHL tumor suppressor protein. Moreover, yeast Elongin C strongly induces the transcriptional elongation activity of mammalian Elongin A. The expression of yeast Elongin C mRNA is dramatically upregulated during sporulation; however, the gene is not essential for sporulation and viability in yeast cell.
authors.affiliation: Center for Tsukuba Advanced Research Alliance, University of Tsukuba, Ibaraki, Japan. taso@tara.tsukuba.ac.jp
authors.forename: T
authors.initials: T
authors.lastname: Aso
authors.forename: M N
authors.initials: MN
authors.lastname: Conrad
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics; D004271:DNA, Fungal / Q000235:genetics; D004331:Drosophila melanogaster / Q000235:genetics*; D000075065:Elongin; D005786:Gene Expression Regulation; D005800:Genes, Fungal; D017344:Genes, Insect; D015698:Genomic Library; D008969:Molecular Sequence Data; D011485:Protein Binding; D011487:Protein Conformation; D012441:Saccharomyces cerevisiae / Q000235:genetics*; D017386:Sequence Homology, Amino Acid; D013045:Species Specificity; D014157:Transcription Factors / Q000235:genetics*; D014158:Transcription, Genetic
chemical_list: D018076:DNA, Complementary; D004271:DNA, Fungal; D000075065:Elongin; D014157:Transcription Factors