Multiple unrelated translocations in a metastatic epimyoepithelial carcinoma of the parotid gland.
We report the cytogenetic, fluorescence in-situ hybridization (FISH) and DNA flow cytometric analysis of a rare metastatic epimyoepithelial carcinoma of the parotid gland to the lung with a clinical course of 29 years. DNA content and FISH analyses of tumor and short-term culture cells showed diploid DNA content and lack of numerical chromosomal abnormalities. Immunohistochemical analysis of the short-term culture cells showed predominantly keratin positive and sparse desmin staining supporting an epithelial rather than myoepithelial origin. Cytogenetic analysis showed 46,XY karyotype with clonal translocations of t(3;22)(q13.2;q13.1), t(1;7)(q21;q22), t(8;9)(p10;p10), and t(5;6) (q35;q21). Our findings indicate that these alterations developed in a diploid stemline during tumor progression and in the epithelial component of this tumor.
el-Naggar
A K
AK
Department of Pathology, University of Texas, M.D. Anderson Cancer Center, Houston 77030, USA.
Lovell
M
M
Ordonez
N G
NG
Killary
A M
AM
D000328:Adult; D002277:Carcinoma / Q000235:genetics* / Q000473:pathology; D002871:Chromosome Banding; D006801:Humans; D007621:Karyotyping; D008207:Lymphatic Metastasis; D008297:Male; D010307:Parotid Neoplasms / Q000235:genetics* / Q000473:pathology; D014178:Translocation, Genetic