Nuclear magnetic resonance structural and ligand binding studies of BLBC, a two-domain fragment of barley lectin.
Plant lectins are useful targets for biophysical studies of protein-carbohydrate recognition, a process of general interest because of its many roles in human physiology. Here, nuclear magnetic resonance (NMR) based structural and carbohydrate binding data on a two-domain fragment of the normally four-domain barley lectin protein are presented. The structural data, while preliminary, clearly shows that the recombinantly produced simplified model system, called BLBC, retains a nativelike fold. However, unlike the full-length parent protein, which is dimeric, BLBC is shown by pulsed-field gradient NMR diffusion studies to be largely monomeric. Still, the fragment retains nativelike carbohydrate binding properties. These properties are examined in some detail using heteronuclear single quantum coherence (HSQC) NMR spectroscopy on a uniformly 15N-labeled sample. Ligand-induced chemical shift changes in the 1H-15N HSQC spectrum are monitored as 15N-labeled BLBC is titrated with increasing concentrations of the unlabeled carbohydrate, N,N',N"-triacetylchitotriose. Well-resolved resonances from the individual domains show that BLBC binds ligand at two distinct and independent ligand binding sites, one in each domain. Binding constants of (1.1 +/- 0.2) x 10(3) M-1 and (0.6 +/- 0.2) x 10(3) M-1 are determined for the B and C domain sites, respectively. These results are discussed in relation to ligand binding studies that have previously been carried out on a highly homologous protein, wheat germ agglutinin.
Weaver
J L
JL
Department of Chemistry, Yale University, New Haven, Connecticut 06511, USA.
Prestegard
J H
JH
D000595:Amino Acid Sequence; D018360:Crystallography, X-Ray; D001467:Hordeum / Q000737:chemistry*; D037102:Lectins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*; D008024:Ligands; D009682:Magnetic Resonance Spectroscopy; D008958:Models, Molecular; D008969:Molecular Sequence Data; D009587:Nitrogen Isotopes; D010446:Peptide Fragments / Q000737:chemistry* / Q000378:metabolism*; D037121:Plant Lectins; D011485:Protein Binding / Q000235:genetics; D017434:Protein Structure, Tertiary; D011522:Protons; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis / Q000737:chemistry
D037102:Lectins; D008024:Ligands; D009587:Nitrogen Isotopes; D010446:Peptide Fragments; D037121:Plant Lectins; D011522:Protons; D011993:Recombinant Fusion Proteins; C076978:barley lectin