High-performance liquid chromatographic determination of orotic acid as its methyl derivative in human urine.
Described is a method for the determination of orotic acid as its methyl ester in human urine. The method involves the use of solid-phase extraction to isolate pyrimidines from urine and derivatization with methanol and sulfuric acid, followed by isocratic high-performance liquid chromatography on a reversed-phase C18 column with UV absorbance detection. The assay is shown to be sufficiently sensitive for use in clinical investigations where elevated orotic acid excretion is suspected.
Fioravanti
A
A
Department of Clinical Medicine, Pathology and Pharmacology, University of Perugia, Italy.
Flaviani
M
M
Gambelunghe
C
C
Micheletti
A
A
Sposito
M
M
Rufini
S
S
D000328:Adult; D001942:Breast Feeding; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D003404:Creatinine / Q000652:urine; D005260:Female; D006801:Humans; D007225:Infant Food; D007231:Infant, Newborn; D016014:Linear Models; D008745:Methylation; D009954:Ornithine Carbamoyltransferase / Q000652:urine; D020163:Ornithine Carbamoyltransferase Deficiency Disease; D009963:Orotic Acid / Q000737:chemistry / Q000652:urine*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013056:Spectrophotometry, Ultraviolet
D009963:Orotic Acid; D003404:Creatinine; D009954:Ornithine Carbamoyltransferase