title: Expression of glycoconjugates in normal and Sjögren's syndrome labial glands.
abstract: Glyconjugate expression in formalin-fixed, paraffin-embedded Sjögren's syndrome labial glands (nine cases) was compared with that in normal glands (12 samples) using a wide panel of lectins. Mucous cells expressed mainly mucous-type glycoproteins including sialyl, fucosyl, galactosyl and galactosaminosyl residues, and some N-linked glycoconjugates. Demilunar cells expressed mainly alpha D-mannose, GlcNAc and Gal beta 1.3-GalNAc. Duct cells and cellular glycocalyx expressed O-linked and N-linked residues. Sjögren's syndrome samples showed a statistically significant increase in the expression of GlcNAc. A significantly decreased expression of Gal beta 1,3-GalNAc and alpha D-mannose, residues not usually present in mucous cells, was found (p < 0.05). This suggests that anatomic rather than functional alterations determine mucous cell impairment and the symptoms in Sjögren's syndrome.
authors.affiliation: Department of Otolaryngology, Faculty of Medicine, Kagoshima University, Japan.
authors.forename: J
authors.initials: J
authors.lastname: Sidagis
authors.forename: K
authors.initials: K
authors.lastname: Ueno
authors.forename: Z H
authors.initials: ZH
authors.lastname: Wang
authors.forename: Y
authors.initials: Y
authors.lastname: Hanamure
authors.forename: S
authors.initials: S
authors.lastname: Furuta
authors.forename: M
authors.initials: M
authors.lastname: Ohyama
mesh_terms: D005260:Female; D006001:Glycoconjugates / Q000378:metabolism*; D006651:Histocytochemistry; D006801:Humans; D037102:Lectins; D008297:Male; D008875:Middle Aged; D012470:Salivary Glands, Minor / Q000378:metabolism* / Q000473:pathology; D012859:Sjogren's Syndrome / Q000378:metabolism* / Q000473:pathology
chemical_list: D006001:Glycoconjugates; D037102:Lectins