title: Premarket evaluation of a commercial glycoprotein G-based enzyme immunoassay for herpes simplex virus type-specific antibodies.
abstract: A new commercial glycoprotein G-based enzyme immunoassay (gG-EIA) was compared with Western blotting (WB) for detection of herpes simplex virus type 1 (HSV-1) or HSV-2 type-specific antibodies in 193 serum samples. Sensitivity for HSV-1 was 95%; specificity was 96%. Sensitivity for HSV-2 was 98%; specificity was 97%. Twelve of 13 serum samples with equivocal gG-EIA results were serotyped by WB.
authors.affiliation: Department of Laboratory Medicine, University of Washington, Seattle, USA. rashley@u.washington.edu
authors.forename: R L
authors.initials: RL
authors.lastname: Ashley
authors.forename: L
authors.initials: L
authors.lastname: Wu
authors.forename: J W
authors.initials: JW
authors.lastname: Pickering
authors.forename: M C
authors.initials: MC
authors.lastname: Tu
authors.forename: L
authors.initials: L
authors.lastname: Schnorenberg
mesh_terms: D000914:Antibodies, Viral / Q000097:blood*; D006801:Humans; D007124:Immunoenzyme Techniques; D018139:Simplexvirus / Q000276:immunology*; D014759:Viral Envelope Proteins / Q000276:immunology*
chemical_list: D000914:Antibodies, Viral; D014759:Viral Envelope Proteins; C045464:glycoprotein G, herpes simplex virus type 2; C050591:glycoprotein gG-1, herpes simplex virus type 1