title: Mannose induces the release of cytopathic factors from Acanthamoeba castellanii.
abstract: Acanthamoeba keratitis is a chronic inflammatory disease of the cornea which is highly resistant to many antimicrobial agents. The pathogenic mechanisms of this disease are poorly understood. However, it is believed that the initial phases in the pathogenesis of Acanthamoeba keratitis involve parasite binding and lysis of the corneal epithelium. These processes were examined in vitro, using Acanthamoeba castellanii trophozoites. Parasites readily adhered to Chinese hamster corneal epithelial cells in vitro; however, parasite binding was strongly inhibited by mannose but not by lactose. Although mannose prevented trophozoite binding, it did not affect cytolysis of corneal epithelial cells. Moreover, mannose treatment induced trophozoites to release cytolytic factors that lysed corneal epithelial cells in vitro. These factors were uniquely induced by mannose because supernatants collected from either untreated trophozoites or trophozoites treated with other sugars failed to lyse corneal cells. The soluble factors were size fractionated in centrifugal concentrators and found to be > or = 100 kDa. Treatment of the supernatants with the serine protease inhibitor phenylmethylsulfonyl fluoride inhibited most, but not all, of the cytopathic activity. These data suggest that the binding of Acanthamoeba to mannosylated proteins on the corneal epithelium may exacerbate the pathogenic cascade by initiating the release of cytolytic factors.
authors.affiliation: Department of Ophthalmology, University of Texas Southwestern Medical Center, Dallas 75235-9057, USA.
authors.forename: H
authors.initials: H
authors.lastname: Leher
authors.forename: R
authors.initials: R
authors.lastname: Silvany
authors.forename: H
authors.initials: H
authors.lastname: Alizadeh
authors.forename: J
authors.initials: J
authors.lastname: Huang
authors.forename: J Y
authors.initials: JY
authors.lastname: Niederkorn
mesh_terms: D000048:Acanthamoeba / Q000378:metabolism* / Q000472:pathogenicity*; D015823:Acanthamoeba Keratitis / Q000378:metabolism / Q000469:parasitology*; D000818:Animals; D002478:Cells, Cultured; D017077:Culture Media, Conditioned / Q000494:pharmacology; D003603:Cytotoxins / Q000302:isolation & purification / Q000378:metabolism*; D004847:Epithelial Cells / Q000469:parasitology; D019573:Epithelium, Corneal / Q000469:parasitology; D005690:Galactose / Q000378:metabolism / Q000494:pharmacology; D007785:Lactose / Q000378:metabolism / Q000494:pharmacology; D008358:Mannose / Q000378:metabolism / Q000494:pharmacology*; D010664:Phenylmethylsulfonyl Fluoride / Q000494:pharmacology
chemical_list: D017077:Culture Media, Conditioned; D003603:Cytotoxins; D010664:Phenylmethylsulfonyl Fluoride; D007785:Lactose; D008358:Mannose; D005690:Galactose