Visualization of chromatin folding patterns in chicken erythrocytes by atomic force microscopy (AFM).
The organization of the higher order structure of chromatin in chicken erythrocytes has been examined with tapping-mode scanning force microscopy under conditions close to their native environment. Reproducible high-resolution AFM images of chromatin compaction at several levels can be demonstrated. An extended beads-on-astring (width of approximately 15-20 nm, height of approximately 2-3 nm for each individual nucleosome) can be consistently observed. Furthermore, superbeads (width of approximately 40 nm, height of approximately 7 nm) are demonstrated. Visualization of the solenoid conformation at the level of 30 nm chromatin fiber is attained either by using AFM or by using electron microscopy. In addition, tightly coiled chromatin fibers (approximately 50-60 nm and approximately 90-110 nm) can be revealed. Our data suggest that the chromatin in the interphase nucleus of chicken erythrocyte represents a high-order conformation and AFM provides useful high-resolution structural information concerning the folding pattern of interphase chromatin fibers.
Qian
R L
RL
Shanghai Institute of Cell Biology, Chinese Academy of Sciences, China.
Liu
Z X
ZX
Zhou
M Y
MY
Xie
H Y
HY
Jiang
C
C
Yan
Z J
ZJ
Li
M Q
MQ
Zhang
Y
Y
Hu
J
J
D000818:Animals; D002645:Chickens; D002843:Chromatin / Q000737:chemistry / Q000378:metabolism*; D004247:DNA / Q000378:metabolism; D004912:Erythrocytes / Q000737:chemistry* / Q000648:ultrastructure; D018625:Microscopy, Atomic Force; D008854:Microscopy, Electron; D009707:Nucleosomes / Q000737:chemistry / Q000648:ultrastructure; D011487:Protein Conformation; D017510:Protein Folding
D002843:Chromatin; D009707:Nucleosomes; D004247:DNA