Preventive action of vitamin E-containing liposomes on cataractogenesis in young adult rats fed a 25% galactose diet.
The preventive action of vitamin E (Vit. E)-containing liposomes on cataractogenesis was examined in male Wistar rats (five weeks old) fed a 25% galactose diet. Vit. E-containing liposomes prepared with dipalmitoylphosphatidylcholine were instilled into both eyes three times a day over a 45-day period. Cataract appeared at 18-day galactose feeding and developed gradually thereafter. Simultaneous Vit. E-containing liposome instillation delayed this cataractogenesis. Lenses of 18-day galactose-fed rats showed decreases in Vit. E and reduced glutathione (GSH) contents and Na+, K(+)-ATPase activity and increases in lipid peroxide (LPO), galactitol, and water contents. Lenses of 45-day galactose fed rats showed decreases in GSH content and Na+,K(+)-ATPase activity and increases in Vit. E, LPO, galactitol, and water contents. Serum Vit. E and cholesterol levels decreased in 18-day galactose-fed rats, while both levels increased in 45-day galactose-fed rats. Simultaneous Vit. E-containing liposome instillation prevented these changes except for the changes of lenticular galactitol and water contents and serum Vit. E and cholesterol levels. These results indicate that simultaneously instilled Vit. E-containing liposomes can delay cataractogenesis in young adult rats fed a 25% galactose diet mainly by the antioxidative action of Vit. E contained in the instilled liposomes.
Ohta
Y
Y
Department of Biochemistry, School of Medicine, Fujita Health University, Toyoake, Aichi, Japan.
Torii
H
H
Yamasaki
T
T
Niwa
T
T
Majima
Y
Y
Ishiguro
I
I
D000818:Animals; D000975:Antioxidants / Q000008:administration & dosage* / Q000378:metabolism; D002386:Cataract / Q000517:prevention & control*; D002784:Cholesterol / Q000097:blood; D004337:Drug Carriers; D005690:Galactose / Q000008:administration & dosage; D005978:Glutathione / Q000378:metabolism; D007908:Lens, Crystalline / Q000378:metabolism; D015227:Lipid Peroxidation; D008081:Liposomes; D008297:Male; D051381:Rats; D017208:Rats, Wistar; D000254:Sodium-Potassium-Exchanging ATPase / Q000378:metabolism; D014810:Vitamin E / Q000008:administration & dosage* / Q000378:metabolism
D000975:Antioxidants; D004337:Drug Carriers; D008081:Liposomes; D014810:Vitamin E; D002784:Cholesterol; D000254:Sodium-Potassium-Exchanging ATPase; D005978:Glutathione; D005690:Galactose