title: Use of a gastric juice-based PCR assay to detect Helicobacter pylori infection in culture-negative patients.
abstract: A gastric juice-based PCR assay was compared with culture, microscopy, and a rapid urease test with specimens from 114 subjects. The PCR and conventional tests were positive for 76 and 62% of the subjects, respectively. The prevalence of gastroduodenal disease and seropositivity for anti-Helicobacter pylori immunoglobulin G were similarly high among conventional-test-positive and PCR-only-positive subjects compared to all-negative ones. The PCR assay is recommended to confirm the H. pylori status of culture-negative peptic-ulcer patients.
authors.affiliation: Second Department of Internal Medicine, University of Tokyo, Japan. yoshida-2im@h.u-tokyo.ac.jp
authors.forename: H
authors.initials: H
authors.lastname: Yoshida
authors.forename: K
authors.initials: K
authors.lastname: Hirota
authors.forename: Y
authors.initials: Y
authors.lastname: Shiratori
authors.forename: T
authors.initials: T
authors.lastname: Nihei
authors.forename: S
authors.initials: S
authors.lastname: Amano
authors.forename: A
authors.initials: A
authors.lastname: Yoshida
authors.forename: O
authors.initials: O
authors.lastname: Kawamata
authors.forename: M
authors.initials: M
authors.lastname: Omata
mesh_terms: D000328:Adult; D000368:Aged; D000907:Antibodies, Bacterial / Q000097:blood; D005260:Female; D005750:Gastric Juice / Q000382:microbiology*; D016481:Helicobacter Infections / Q000175:diagnosis*; D016480:Helicobacter pylori; D006801:Humans; D007074:Immunoglobulin G / Q000097:blood; D008297:Male; D008875:Middle Aged; D016133:Polymerase Chain Reaction
chemical_list: D000907:Antibodies, Bacterial; D007074:Immunoglobulin G