Interaction of a Golgi-associated kinesin-like protein with Rab6.
Rab guanosine triphosphatases regulate vesicular transport and membrane traffic within eukaryotic cells. Here, a kinesin-like protein that interacts with guanosine triphosphate (GTP)-bound forms of Rab6 was identified. This protein, termed Rabkinesin-6, was localized to the Golgi apparatus and shown to play a role in the dynamics of this organelle. The carboxyl-terminal domain of Rabkinesin-6, which contains the Rab6-interacting domain, inhibited the effects of Rab6-GTP on intracellular transport. Thus, a molecular motor is a potential effector of a Rab protein, and coordinated action between members of these two families of proteins could control membrane dynamics and directional vesicular traffic.
Echard
A
A
Unité Mixte de Recherche CNRS 144 et 168, Institut Curie, 26 rue d'Ulm, 75248 Paris Cedex 05, France.
Jollivet
F
F
Martinez
O
O
Lacapère
J J
JJ
Rousselet
A
A
Janoueix-Lerosey
I
I
Goud
B
B
D000251:Adenosine Triphosphatases / Q000378:metabolism; D000469:Alkaline Phosphatase / Q000378:metabolism; D000595:Amino Acid Sequence; D001692:Biological Transport; D002352:Carrier Proteins / Q000378:metabolism*; D004721:Endoplasmic Reticulum / Q000378:metabolism; D006056:Golgi Apparatus / Q000737:chemistry / Q000378:metabolism* / Q000648:ultrastructure; D006160:Guanosine Triphosphate / Q000378:metabolism; D006367:HeLa Cells; D006801:Humans; D016547:Kinesins / Q000032:analysis / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D008870:Microtubules / Q000378:metabolism / Q000648:ultrastructure; D008969:Molecular Sequence Data; D008970:Molecular Weight; D020691:rab GTP-Binding Proteins; D018631:ras Proteins / Q000378:metabolism*
D002352:Carrier Proteins; C410089:KIF20A protein, human; C094135:Rab6 protein; D006160:Guanosine Triphosphate; D000469:Alkaline Phosphatase; D000251:Adenosine Triphosphatases; D016547:Kinesins; D020691:rab GTP-Binding Proteins; D018631:ras Proteins