Preferential expression of 5-HT1D over 5-HT1B receptors during early embryogenesis.
The cloning and pharmacological characterization of mouse 5-HT1D receptors as well as the comparative analysis of its embryonic expression vs that of 5-HT1B receptors are reported. High densities of both 5-HT1D receptors mRNA and specific 5-HT1D binding sites were detected at 8, 9.5, 10.5 and 13.5 days of prenatal development. In contrast, no specific 5-HT1B binding sites could be detected until 13.5 days of development, when they were present at lower levels than 5-HT1D receptors. This differs markedly from the situation in the adult brain, in which 5-HT1B receptors are present at a much higher density than the 5-HT1D subtype. These data suggest the involvement of 5-HT1D receptors in the mitogenic and proliferative effects of serotonin during early embryonic development.
Bolaños-Jiménez
F
F
Institut de Génétique et de Biologie Moléculaire et Cellulaire, Université Louis Pasteur de Strasbourg, CNRS, INSERM, BP 163, Illkirch, France.
Choi
D S
DS
Maroteaux
L
L
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D019556:COS Cells; D017124:Conserved Sequence; D017931:DNA Primers; D005314:Embryonic and Fetal Development; D018507:Gene Expression Regulation, Developmental; D015698:Genomic Library; D006801:Humans; D051379:Mice; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D014176:Protein Biosynthesis; D012333:RNA, Messenger / Q000096:biosynthesis; D011869:Radioligand Assay; D051381:Rats; D044302:Receptor, Serotonin, 5-HT1B; D044346:Receptor, Serotonin, 5-HT1D; D011985:Receptors, Serotonin / Q000096:biosynthesis* / Q000737:chemistry / Q000235:genetics; D011994:Recombinant Proteins / Q000737:chemistry / Q000378:metabolism; D016415:Sequence Alignment; D014158:Transcription, Genetic; D014162:Transfection
D017931:DNA Primers; C470302:HTR1B protein, human; D012333:RNA, Messenger; D044302:Receptor, Serotonin, 5-HT1B; D044346:Receptor, Serotonin, 5-HT1D; D011985:Receptors, Serotonin; D011994:Recombinant Proteins