[A simple method for RNA isolation and purification].
RNAs from Escherichia coli cells, Syrian hamster kidney cells, foot-and-mouth disease virus, and Newcastle disease virus were isolated using glass fiber filters GF/F or GF/C. The RNA was reversibly adsorbed on the filters in the presence of 2 M guanidine thiocyanate and 50% ethanol (or isopropanol) and eluted with water. The fraction composition of the isolated RNA depended on the guanidine thiocyanate and alcohol concentrations in the adsorption and washing procedures. The RNA preparations obtained by this method can be used in reverse transcription and reverse transcription-polymerase chain reaction without additional purification.
Gribanov
O G
OG
Shcherbakov
A V
AV
Perevozchikova
N A
NA
Drygin
V V
VV
Gusev
A A
AA
D000818:Animals; D006224:Cricetinae; D004926:Escherichia coli / Q000235:genetics; D005374:Filtration / Q000379:methods; D006146:Guanidines; D007668:Kidney / Q000737:chemistry; D008647:Mesocricetus; D010849:Picornaviridae / Q000235:genetics; D012313:RNA / Q000302:isolation & purification*; D012329:RNA, Bacterial / Q000302:isolation & purification*; D012367:RNA, Viral / Q000302:isolation & purification*; D018108:Respirovirus / Q000235:genetics; D013861:Thiocyanates
D006146:Guanidines; D012329:RNA, Bacterial; D012367:RNA, Viral; D013861:Thiocyanates; C054436:guanidine thiocyanate; D012313:RNA