Different modulation of steroidogenesis and prostaglandin production in frog ovary in vitro by ACE and ANG II.
Our aim was to study the role of angiotensin-converting enzyme (ACE) and angiotensin II (ANG II) on ovarian steroidogenesis and prostaglandin production of amphibian. Hormonal effects of ACE, ACE inhibitors, synthetic bullfrog angiotensin I (ANG I), and [Val5]ANG II were compared on frog ovaries of postreproductive and prereproductive periods. Very high ACE activity was found in ovary of water frog (Rana esculenta) compared with other frog tissues, and this activity was inhibited by the typical ACE inhibitors, captopril and lisinopril. Frog ovary tissue in postreproductive and prereproductive periods was incubated in vitro in the presence of ACE (2.5 mU/ml), captopril (0.1 mM), lisinopril (0.1 mM), [Val5]ANG II (1 microM), and synthetic bullfrog ANG I (1 microM). Production of 17 beta-estradiol, progesterone, androgens, and prostaglandins E2 and F2 alpha was determined. The data showed a modulation of 17 beta-estradiol, progesterone, and prostaglandin E2 production by ovary ACE; on the other hand, [Val5]ANG II modulated the production of progesterone and prostaglandin F2 alpha, whereas androgen production was not influenced. The present in vitro studies suggest the existence of two pathways independently regulated by ACE and ANG II modulating ovarian steroidogenesis and prostaglandin production.
Bramucci
M
M
Department of Molecular, Cellular and Animal Biology, University of Camerino, MC, Italy.
Miano
A
A
Gobbetti
A
A
Zerani
M
M
Quassinti
L
L
Maccari
E
E
Murri
O
O
Amici
D
D
D000728:Androgens / Q000096:biosynthesis; D000803:Angiotensin I / Q000494:pharmacology; D000804:Angiotensin II / Q000494:pharmacology*; D000806:Angiotensin-Converting Enzyme Inhibitors / Q000494:pharmacology*; D000818:Animals; D002216:Captopril / Q000494:pharmacology; D015237:Dinoprost / Q000096:biosynthesis; D015232:Dinoprostone / Q000096:biosynthesis; D004958:Estradiol / Q000096:biosynthesis; D005260:Female; D012739:Gonadal Steroid Hormones / Q000096:biosynthesis*; D007668:Kidney / Q000201:enzymology; D017706:Lisinopril / Q000494:pharmacology; D008168:Lung / Q000201:enzymology; D010053:Ovary / Q000187:drug effects / Q000201:enzymology / Q000378:metabolism*; D007703:Peptidyl-Dipeptidase A / Q000378:metabolism* / Q000494:pharmacology; D011374:Progesterone / Q000096:biosynthesis; D011453:Prostaglandins / Q000096:biosynthesis*; D011817:Rabbits; D011892:Rana catesbeiana; D011893:Rana esculenta
D000728:Androgens; D000806:Angiotensin-Converting Enzyme Inhibitors; D012739:Gonadal Steroid Hormones; D011453:Prostaglandins; D000804:Angiotensin II; D011374:Progesterone; D004958:Estradiol; D000803:Angiotensin I; D002216:Captopril; D015237:Dinoprost; D017706:Lisinopril; D007703:Peptidyl-Dipeptidase A; D015232:Dinoprostone