Effect of hydrocortisone, methylprednisolone aceponate and momethasone furoate on collagen synthesis in human skin in vivo.
BACKGROUND
Topical corticoids decrease de novo collagen synthesis in the human skin.


OBJECTIVE
We studied the effect of three corticoids, hydrocortisone (HC), methylprednisolone aceponate (MPA) and momethasone furoate (MMF) on the de novo synthesis of type I and III collagens.


METHODS
Fifteen healthy male volunteers treated four areas marked on their abdominal skin for 1 week. HC was applied twice a day, MPA and MMF once a day plus vehicle once a day and vehicle twice a day. After the treatment, suction blisters were induced on the treated areas, the suction blister fluid (SBF) was collected and procollagen propeptides of type I and III procollagens (PINP, PIIINP, respectively) were analyzed by radioimmunological assays. The protein concentration in SBF was determined by a colorimetric method.


RESULTS
All the corticoids studied decreased the procollagen propeptide concentrations in SBF. HC decreased PINP concentration by 66%, MPA by 68% and MMF by 72%. HC decreased PIIINP by 62%, MPA by 68% and MMF by 72%. The protein concentration in SBF was decreased by 11-15% by these topical corticoids.


CONCLUSIONS
HC decreases the concentration of procollagen propeptides in human skin in males to nearly the same extent as MPA and MMF.
Haapasaari
K M
KM
Department of Dermatology, University of Oulu, Finland.
Risteli
J
J
Karvonen
J
J
Oikarinen
A
A
D000287:Administration, Topical; D000328:Adult; D000893:Anti-Inflammatory Agents / Q000494:pharmacology*; D003094:Collagen / Q000096:biosynthesis*; D006801:Humans; D006854:Hydrocortisone / Q000494:pharmacology*; D008297:Male; D008775:Methylprednisolone / Q000031:analogs & derivatives* / Q000494:pharmacology; D000068656:Mometasone Furoate; D010446:Peptide Fragments / Q000378:metabolism; D011244:Pregnadienediols / Q000494:pharmacology*; D011347:Procollagen / Q000378:metabolism; D012867:Skin / Q000187:drug effects* / Q000378:metabolism*
D000893:Anti-Inflammatory Agents; D010446:Peptide Fragments; D011244:Pregnadienediols; D011347:Procollagen; C047202:procollagen Type I N-terminal peptide; C041876:procollagen Type III-N-terminal peptide; D000068656:Mometasone Furoate; D003094:Collagen; C078007:methylprednisolone aceponate; D006854:Hydrocortisone; D008775:Methylprednisolone