title: Vitamin C supplementation restores the impaired vitamin E status of guinea pigs fed oxidized frying oil.
abstract: To investigate the effect of dietary oxidized frying oil (OFO) on tissue retention of vitamin C, and to explore the effect of vitamin C supplementation on tissue vitamin E concentrations and lipid peroxidation, male weanling guinea pigs were divided into four groups. Guinea pigs were fed 15% OFO diets supplemented with vitamin C at 300, 600 or 1500 mg/kg diet. Control animals were fed a diet containing 15% fresh untreated soybean oil with 300 mg/kg of vitamin C. After 60 d of feeding, body weight gain, food intake, feed efficiency and plasma triglyceride concentration were significantly lower in guinea pigs fed OFO diets than in controls (P < 0.05). However, plasma cholesterol concentration was highest in guinea pigs fed the OFO diet supplemented with 300 mg/kg vitamin C. Increasing vitamin C in OFO diets significantly reduced plasma cholesterol concentration. Plasma and tissue vitamins C and E concentrations were significantly lower in the OFO-fed guinea pigs receiving 300 mg/kg vitamin C than in controls. Greater levels of supplemental vitamin C increased tissue vitamins C and E. Guinea pigs fed OFO diets had significantly higher tissue levels of thiobarbituric acid reactive substances (TBARS) (P < 0.05) than controls. Our results demonstrated that OFO feeding, which impaired alpha-tocopherol retention and increased TBARS, could be alleviated somewhat by vitamin C supplementation.
authors.affiliation: School of Nutrition and Health Science, Taipei Medical College, Taipei, Taiwan, R.O.C.
authors.forename: J F
authors.initials: JF
authors.lastname: Liu
authors.forename: Y W
authors.initials: YW
authors.lastname: Lee
mesh_terms: D000818:Animals; D001205:Ascorbic Acid / Q000008:administration & dosage* / Q000097:blood / Q000378:metabolism; D002784:Cholesterol / Q000097:blood; D004042:Dietary Fats, Unsaturated / Q000008:administration & dosage*; D004435:Eating; D006168:Guinea Pigs; D006358:Hot Temperature; D008297:Male; D009928:Organ Specificity; D010084:Oxidation-Reduction; D012044:Regression Analysis; D013024:Soybean Oil / Q000008:administration & dosage; D017392:Thiobarbituric Acid Reactive Substances / Q000378:metabolism; D014280:Triglycerides / Q000097:blood; D014810:Vitamin E / Q000378:metabolism*; D015430:Weight Gain
chemical_list: D004042:Dietary Fats, Unsaturated; D017392:Thiobarbituric Acid Reactive Substances; D014280:Triglycerides; D014810:Vitamin E; D013024:Soybean Oil; D002784:Cholesterol; D001205:Ascorbic Acid