title: Role of vesicle-associated syntaxin 5 in the assembly of pre-Golgi intermediates.
abstract: Syntaxins are thought to function during vesicular transport as receptors on the target membrane and to contribute to the specificity of membrane docking and fusion by interacting with vesicle-associated receptors. Here, syntaxin 5 (Syn5) was shown to be an integral component of endoplasmic reticulum-derived transport vesicles. This pool, but not the target, Golgi-associated Syn5 pool, was essential for the assembly of vesicular-tubular pre-Golgi intermediates and the delivery of cargo to the Golgi. The requirement for vesicle-associated Syn5 in transport suggests a reevaluation of the basis for operation of the early secretory pathway.
authors.affiliation: Department of Cell Biology, The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, CA 92037, USA.
authors.forename: T
authors.initials: T
authors.lastname: Rowe
authors.forename: C
authors.initials: C
authors.lastname: Dascher
authors.forename: S
authors.initials: S
authors.lastname: Bannykh
authors.forename: H
authors.initials: H
authors.lastname: Plutner
authors.forename: W E
authors.initials: WE
authors.lastname: Balch
mesh_terms: D000255:Adenosine Triphosphate / Q000378:metabolism; D000818:Animals; D000906:Antibodies; D001692:Biological Transport; D002352:Carrier Proteins / Q000378:metabolism; D002460:Cell Line; D004721:Endoplasmic Reticulum / Q000378:metabolism*; D006056:Golgi Apparatus / Q000378:metabolism* / Q000648:ultrastructure; D037241:Mannose-Binding Lectins; D008561:Membrane Fusion; D008562:Membrane Glycoproteins; D008565:Membrane Proteins / Q000276:immunology / Q000378:metabolism*; D052066:N-Ethylmaleimide-Sensitive Proteins; D015388:Organelles / Q000378:metabolism; D050765:Qa-SNARE Proteins; D050766:Qb-SNARE Proteins; D050767:Qc-SNARE Proteins; D050683:R-SNARE Proteins; D051381:Rats; D050600:SNARE Proteins; D033921:Vesicular Transport Proteins; D014721:Vesicular stomatitis Indiana virus / Q000502:physiology; D014759:Viral Envelope Proteins / Q000378:metabolism
chemical_list: D000906:Antibodies; C098783:BET1 protein, rat; D002352:Carrier Proteins; C028391:G protein, vesicular stomatitis virus; C491343:Gosr2 protein, rat; C084004:LMAN1 protein, human; D037241:Mannose-Binding Lectins; D008562:Membrane Glycoproteins; D008565:Membrane Proteins; D050765:Qa-SNARE Proteins; D050766:Qb-SNARE Proteins; D050767:Qc-SNARE Proteins; D050683:R-SNARE Proteins; D050600:SNARE Proteins; C098784:Sec22a protein, rat; D033921:Vesicular Transport Proteins; D014759:Viral Envelope Proteins; D000255:Adenosine Triphosphate; D052066:N-Ethylmaleimide-Sensitive Proteins; C498737:Nsf protein, rat