CCAAT/enhancer-binding protein delta gene expression is mediated by autoregulation through downstream binding sites.
CCAAT/enhancer-binding protein delta (C/EBP delta) transcription factor is sharply induced at the early stage of the acute phase response. We previously reported that the C/EBP delta gene expression is induced by the acute-phase response factor/signal transducers and activators of transcription 3 (APRF/STAT3). However, the expression level of the C/EBP delta gene is relatively high up to several hours after the stimulation, whereas APRF/STAT3 is inactivated within one hour. In this report, we identified the two C/EBP delta binding sites at the downstream region of this gene. The binding analysis revealed that both of these sites bound recombinant C/EBP delta protein. A cotransfection analysis identified these sites as the cis-elements for the autoregulation. We conclude that the C/EBP delta gene is activated by APRF/STAT3, and the expression level is then maintained by an autoregulation mechanism.
Yamada
T
T
Laboratory of Environmental Biochemistry, School of Pharmaceutical Sciences, Osaka University, Japan.
Tsuchiya
T
T
Osada
S
S
Nishihara
T
T
Imagawa
M
M
D000818:Animals; D001483:Base Sequence; D001665:Binding Sites; D022762:CCAAT-Enhancer-Binding Proteins; D004252:DNA Mutational Analysis; D004268:DNA-Binding Proteins / Q000096:biosynthesis*; D005786:Gene Expression Regulation; D017930:Genes, Reporter; D006801:Humans; D008113:Liver Neoplasms; D008969:Molecular Sequence Data; D009687:Nuclear Proteins / Q000096:biosynthesis*; D011485:Protein Binding; D051381:Rats; D017384:Sequence Deletion; D014157:Transcription Factors / Q000096:biosynthesis*; D014407:Tumor Cells, Cultured
D022762:CCAAT-Enhancer-Binding Proteins; D004268:DNA-Binding Proteins; D009687:Nuclear Proteins; D014157:Transcription Factors