Rapid and reliable measurement of highly elevated blood ammonia concentrations in children.
Newborns and children may suffer from extremely high ammonia levels in their blood. We evaluated a fast, reliable micromethod, based on the Blood Ammonia Checker II (BAC II) in combination with the dilution with fresh whole blood. Comparison of the proposed method with an enzymatic method revealed a statistically significant correlation. We conclude that the dilution of patient's blood with fresh whole blood extends the measuring range of ammonia on the BAC II analyzer from 286 mumol/l to about 700 mumol/l.
de Keijzer
M H
MH
Department of Clinical Chemistry, University Hospital St. Radboud, Nijmegen, The Netherlands.
Jakobs
B S
BS
Brandts
R W
RW
Hofs
M T
MT
Trijbels
F J
FJ
Smeitink
J A
JA
D000641:Ammonia / Q000097:blood*; D001774:Blood Chemical Analysis / Q000379:methods*; D002648:Child; D006801:Humans; D007231:Infant, Newborn; D008832:Microchemistry / Q000379:methods; D011933:Reagent Kits, Diagnostic; D015203:Reproducibility of Results
D011933:Reagent Kits, Diagnostic; D000641:Ammonia