Cerebral cortical phospholipase A2 activity of senescence-accelerated mouse is increased in an age-dependent manner.
The PLA2 activities of both the cytosolic and the membrane fractions from the cerebral cortex of 17-month old senescence-accelerated mouse-prone/10 (SAM-P/10) were significantly increased by 1.3 times compared with those of 2-month old mice. The PLA2 activities were independent of Ca2+ ion and not affected either by dithiothreitol (DTT) or by trifluoromethyl ketone analogs of arachidonic acid (AACOCF3). The PLA2 activities were eluted in a single peak of molecular mass of 170 kDa, using a gel filtration column. These findings suggest that the enhanced PLA2 activity may be related to neuronal degeneration and accelerated senescence of SAM-P/10, and cerebral cortical PLA2 activity categorized as a Ca2+-independent PLA2.
Kim
S S
SS
Department of Pharmacology, College of Medicine, Neuroscience Research Institute, Seoul National University, South Korea.
Kim
D K
DK
Suh
Y H
YH
D000375:Aging / Q000235:genetics / Q000502:physiology*; D000818:Animals; D001095:Arachidonic Acids / Q000494:pharmacology; D002118:Calcium / Q000494:pharmacology; D002540:Cerebral Cortex / Q000201:enzymology* / Q000254:growth & development; D004229:Dithiothreitol / Q000494:pharmacology; D008297:Male; D051379:Mice; D008815:Mice, Inbred Strains; D009410:Nerve Degeneration; D010741:Phospholipases A / Q000378:metabolism*; D054467:Phospholipases A2
D001095:Arachidonic Acids; D010741:Phospholipases A; D054467:Phospholipases A2; D002118:Calcium; D004229:Dithiothreitol