Expression of genes encoding membrane-bound hydrogenase in Pseudomonas hydrogenovora under autotrophic condition is dependent on two different promoters.
Expression of a membrane-bound hydrogenase of the hydrogen-utilizing bacterium Pseudomonas hydrogenovora was induced specifically in autotrophic condition. Dot blot and primer extension analysis showed that the transcription of the hydrogenase gene started from the region upstream of a hydrogenase structural gene, hupS, which contained three putative sigma54-type promoter sequences (Phup1, Phup2, and Phup3). The lacZ-fusion analysis of the hupS-upstream region combined with site-directed mutagenesis showed that Phup1 and Phup3 would be essential for a transcriptional initiation. It was also found that the region upstream of Phup sequences was concerned with regulation of transcription.
Ohtsuki
T
T
Department of Applied Biology, Faculty of Textile Science and Technology, Shinshu University, Nagano, Japan.
Shimosaka
M
M
Okazaki
M
M
D001483:Base Sequence; D017931:DNA Primers / Q000032:analysis; D015971:Gene Expression Regulation, Enzymologic / Q000235:genetics*; D006864:Hydrogenase / Q000235:genetics* / Q000378:metabolism; D007763:Lac Operon; D008565:Membrane Proteins / Q000235:genetics; D008969:Molecular Sequence Data; D016297:Mutagenesis, Site-Directed; D010957:Plasmids; D011401:Promoter Regions, Genetic; D011549:Pseudomonas / Q000201:enzymology*; D014158:Transcription, Genetic; D001616:beta-Galactosidase / Q000378:metabolism
D017931:DNA Primers; D008565:Membrane Proteins; D006864:Hydrogenase; D001616:beta-Galactosidase