S-100 proteins in trimethyltin-induced neurodegeneration in the rat hippocampus. An immunochemical and immunocytochemical study.
After acute trimethyltin (TMT) intoxication (21 d after a single i.p. injection at a dose of 8 mg/kg) the histological, immunohistochemical, and immunochemical investigation of adult rat hippocampus showed a distinct pattern of neuronal loss, and an increase in both glial fibrillary acidic protein- (GFAP) immunoreactive cells and GFAP concentration, as expected. S-100-immunoreactive cells also increased markedly, whereas the concentration of S-100 increased even more than that of GFAP. The data show that S-100 is an index of glial reaction to damage after TMT intoxication and suggest the potential usefulness of exploring the possibility that it may play a role in induced neurodegenerative processes.
Vinesi
P
P
Institute of Histology, Catholic University, Rome, Italy.
Geloso
M C
MC
Michetti
F
F
D000818:Animals; D015153:Blotting, Western; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D005904:Glial Fibrillary Acidic Protein / Q000378:metabolism; D006624:Hippocampus / Q000187:drug effects / Q000378:metabolism*; D007150:Immunohistochemistry; D009410:Nerve Degeneration / Q000378:metabolism* / Q000517:prevention & control*; D009457:Neuroglia / Q000187:drug effects / Q000502:physiology; D009474:Neurons / Q000187:drug effects / Q000502:physiology; D051381:Rats; D017208:Rats, Wistar; D009418:S100 Proteins / Q000378:metabolism*; D014298:Trimethyltin Compounds / Q000633:toxicity*
D005904:Glial Fibrillary Acidic Protein; D009418:S100 Proteins; D014298:Trimethyltin Compounds; C046488:trimethyltin