Expression of a gene encoding a ribosomal p40 protein and identification of an active promoter site.
The promoter of a gene encoding a ribosome-associated protein of 40 kDa from Arabidopsis thaliana (A-p40) was sequenced and the expression of the gene studied. A-p40 was expressed in the same organs and with the same variations as the eukaryotic elongation factor 1 alpha (eEF1A), another gene coding for a protein involved in translation Arabidopsis plants transformed with a beta-glucuronidase (GUS) gene driven by the A-p40 promoter confirm that A-p40 is expressed in actively dividing and growing cells. eEF1A promoter-GUS fusions have the same pattern of expression. Comparison of cis-acting elements from A-p40 and eEF1A revealed some common elements. A-p40 promoter deletions and transient gene expression in transfected Arabidopsis protopasts allowed the identification of trap40, a cis-acting element regulating gene expression. Gel retardation experiments indicate that eEF1A and A-p40 are regulated by different cis-acting elements. The role of such elements is discussed.
Scheer
I
I
UMR 5546 Centre National de la Recherche Scientifique-Université Paul Sabatier, Toulouse, France.
Ludevid
M D
MD
Regad
F
F
Lescure
B
B
Pont-Lezica
R F
RF
D017360:Arabidopsis / Q000235:genetics*; D001483:Base Sequence; D018506:Gene Expression Regulation, Plant; D008969:Molecular Sequence Data; D009928:Organ Specificity / Q000235:genetics; D010940:Plant Proteins / Q000096:biosynthesis / Q000235:genetics*; D011401:Promoter Regions, Genetic; D012269:Ribosomal Proteins / Q000096:biosynthesis / Q000235:genetics*; D017422:Sequence Analysis, DNA; D017384:Sequence Deletion
D010940:Plant Proteins; D012269:Ribosomal Proteins; C086117:Rpsa protein, rat