Functional expression of a mammalian odorant receptor.
Candidate mammalian odorant receptors were first cloned some 6 years ago. The physiological function of these receptors in initiating transduction in olfactory receptor neurons remains to be established. Here, a recombinant adenovirus was used to drive expression of a particular receptor gene in an increased number of sensory neurons in the rat olfactory epithelium. Electrophysiological recording showed that increased expression of a single gene led to greater sensitivity to a small subset of odorants.
Zhao
H
H
Interdepartmental Neuroscience Program, Yale University, New Haven, CT 06510, USA.
Ivic
L
L
Otaki
J M
JM
Hashimoto
M
M
Mikoshiba
K
K
Firestein
S
S
D000256:Adenoviridae / Q000235:genetics / Q000502:physiology; D000447:Aldehydes / Q000378:metabolism / Q000494:pharmacology*; D000818:Animals; D004594:Electrophysiology; D005260:Female; D015870:Gene Expression; D005822:Genetic Vectors; D049452:Green Fluorescent Proteins; D008164:Luminescent Proteins / Q000032:analysis / Q000235:genetics; D008297:Male; D009812:Odorants; D018034:Olfactory Receptor Neurons / Q000502:physiology* / Q000821:virology; D018408:Patch-Clamp Techniques; D051381:Rats; D017207:Rats, Sprague-Dawley; D018035:Receptors, Odorant / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D011994:Recombinant Proteins
D000447:Aldehydes; D008164:Luminescent Proteins; D018035:Receptors, Odorant; D011994:Recombinant Proteins; C109883:odorant receptor 17; D049452:Green Fluorescent Proteins; C031639:caprylic aldehyde