title: A period of anaerobic exercise increases the accumulation of reactive carbonyl derivatives in the lungs of rats.
abstract: It is known that acute physical exercise may have diverse pathophysiological consequences in various organs due to free radical formation. We have investigated whether a period of anaerobic running to exhaustion in rats results in oxidative modification of proteins in the lungs. Six rats of an exercised group (E) ran for two periods of 5 min at a speed of 30 m.min-1 followed by a recovery period of 5 min, and then by a third period of running to exhaustion. Reactive carbonyl derivatives (RCD) were measured by the Western blot technique on lungs of E and control (C) rats. In addition, the activity of glutamine synthetase (GS) was also monitored as marker of oxidative damage to proteins. This investigation revealed significant exercise-induced increases in accumulation of RCD in the lungs of the E group compared with the C group. The RCD signals were visibly stronger in proteins with molecular weight of 55 kDa and 32 kDa. The activity of GS was higher by about 30% in E rats than in C rats. The present data suggest that anaerobic exercise induces protein oxidation in the lungs.
authors.affiliation: Laboratory of Exercise Physiology, Institute of Sport and Health Sciences, University of Tsukuba, Tsukuba.
authors.forename: Z
authors.initials: Z
authors.lastname: Radák
authors.forename: A
authors.initials: A
authors.lastname: Nakamura
authors.forename: H
authors.initials: H
authors.lastname: Nakamoto
authors.forename: K
authors.initials: K
authors.lastname: Asano
authors.forename: H
authors.initials: H
authors.lastname: Ohno
authors.forename: S
authors.initials: S
authors.lastname: Goto
mesh_terms: D000693:Anaerobiosis; D000818:Animals; D015153:Blotting, Western; D005609:Free Radicals; D005974:Glutamate-Ammonia Ligase / Q000378:metabolism; D006835:Hydrazones / Q000378:metabolism*; D008168:Lung / Q000378:metabolism*; D008297:Male; D008970:Molecular Weight; D010084:Oxidation-Reduction; D018384:Oxidative Stress; D005082:Physical Exertion / Q000502:physiology*; D011506:Proteins / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar
chemical_list: D005609:Free Radicals; D006835:Hydrazones; D011506:Proteins; D005974:Glutamate-Ammonia Ligase