Effect of combination treatment of 15 cGy total body irradiation and OK-432 on spontaneous lung metastasis and mitogenic response of splenocytes in mice.
We investigated whether the combination treatment of 15 cGy total body irradiation (TBI) and a streptococcal preparation, OK-432, synergistically suppresses spontaneous lung metastasis and augments phytohemagglutinin (PHA) and concanavalin A (Con A) responses of splenocytes in WHT/Ht mice. TBI with 15 cGy was carried out 20 days after subcutaneous injection of squamous cell carcinoma cells into a hind leg. Lung colony number was counted 40 days after tumor injection. For PHA and Con A responses, mice were killed 4 hr after 15 cGy TBI. The combination treatment of 15 cGy TBI and OK-432 was most effective when OK-432 was administered 2 days before 15 cGy TBI. The combination treatment decreased the lung colony number to 29.9% of the control number. OK-432 slightly increased the PHA and Con A responses, and 15 cGy TBI did not increase them. However, when these two were combined, the PHA and Con A responses were significantly increased to 393% and 278% of the control levels, respectively. It was suggested that TBI and OK-432 acted synergistically in suppressing the lung metastasis and mitogenic response of splenocytes.
Hosoi
Y
Y
Department of Radiology, Tohoku University School of Medicine, Sendai, Japan.
Ishii
K
K
Yamada
S
S
Ono
T
T
Sakamoto
K
K
D000818:Animals; D000970:Antineoplastic Agents / Q000494:pharmacology / Q000627:therapeutic use*; D002294:Carcinoma, Squamous Cell / Q000188:drug therapy* / Q000378:metabolism / Q000532:radiotherapy*; D002478:Cells, Cultured; D003131:Combined Modality Therapy; D004195:Disease Models, Animal; D004307:Dose-Response Relationship, Radiation; D005260:Female; D006133:Growth Substances / Q000502:physiology / Q000528:radiation effects; D008175:Lung Neoplasms / Q000188:drug therapy* / Q000532:radiotherapy* / Q000556:secondary; D051379:Mice; D008815:Mice, Inbred Strains; D010844:Picibanil / Q000627:therapeutic use*; D011829:Radiation Dosage; D013154:Spleen / Q000166:cytology / Q000187:drug effects* / Q000528:radiation effects; D014916:Whole-Body Irradiation
D000970:Antineoplastic Agents; D006133:Growth Substances; D010844:Picibanil