Induction of an antigen-specific, CD1-restricted cytotoxic T lymphocyte response In vivo.
The majority of T cell responses are restricted to peptide antigens bound by polymorphic major histocompatibility complex (MHC) molecules. However, peptide antigens can be presented to T cells by murine non-MHC-encoded CD1d (mCD1) molecules, and human T cell lines specific for nonpeptide antigens presented on CD1 isoforms have been identified. It is shown here that antigen-specific, mCD1-restricted lymphocytes can be generated in vivo by immunizing mice with a combination of plasmids encoding chicken ovalbumin, murine CD1d, and costimulatory molecules. Splenocytes from immunized mice have CD1d-restricted, MHC- unrestricted, ovalbumin-specific cytolytic activity that can be inhibited by anti-CD1 antibodies as well as a competing CD1-binding peptide. These results suggest a physiologic role for murine CD1d to present exogenous protein antigens.
Lee
D J
DJ
Department of Medicine and The Sam and Rose Stein Institute for Research on Aging, University of California, San Diego, La Jolla, California 92093-0663, USA.
Abeyratne
A
A
Carson
D A
DA
Corr
M
M
D000595:Amino Acid Sequence; D000818:Animals; D000906:Antibodies / Q000276:immunology / Q000494:pharmacology; D017951:Antigen Presentation / Q000502:physiology; D018949:Antigens, CD1 / Q000276:immunology*; D016827:CD8 Antigens / Q000276:immunology; D007114:Immunization; D008285:Major Histocompatibility Complex / Q000276:immunology; D051379:Mice; D008969:Molecular Sequence Data; D010047:Ovalbumin / Q000235:genetics / Q000276:immunology; D010455:Peptides / Q000276:immunology / Q000494:pharmacology; D010957:Plasmids / Q000235:genetics / Q000276:immunology; D011485:Protein Binding; D013154:Spleen / Q000276:immunology; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology / Q000378:metabolism*; D014162:Transfection / Q000235:genetics
D000906:Antibodies; D018949:Antigens, CD1; D016827:CD8 Antigens; D010455:Peptides; D010047:Ovalbumin