Complement classical pathway expression by human skeletal myoblasts in vitro.
Human myoblasts express immunological properties in vitro and we have previously reported that they produce Complement (C) components of the alternative pathway. Myoblasts activate the classical pathway but are fully protected against C attack by the expression of major C regulators. In order to fully understand the relationship between myoblasts and C, we here report the biosynthesis of C components of the classical pathway by skeletal muscle cells. Human myoblasts in vitro produced C1q, C1r, C1s, C2 and C4 constitutively and all syntheses were upregulated after stimulation with IFN-gamma. We suggest that human myoblasts may constitute a local source of C and therefore C could be implicated in inflammatory or physiopathological processes developed in skeletal muscle.
Legoedec
J
J
Institut Fédératif de Recherches Multidisciplinaires sur les Peptides (nø. 23), INSERM U-78, BP 73, Bois-Guillaume, France.
Gasque
P
P
Jeanne
J F
JF
Scotte
M
M
Fontaine
M
M
D015153:Blotting, Western; D002478:Cells, Cultured; D015922:Complement C1q / Q000235:genetics / Q000276:immunology / Q000378:metabolism; D015923:Complement C1r / Q000235:genetics / Q000276:immunology / Q000378:metabolism; D003173:Complement C1s / Q000235:genetics / Q000276:immunology / Q000378:metabolism; D003175:Complement C2 / Q000235:genetics / Q000276:immunology / Q000378:metabolism; D003181:Complement C4 / Q000235:genetics / Q000276:immunology / Q000378:metabolism; D003171:Complement Pathway, Classical / Q000276:immunology*; D003165:Complement System Proteins / Q000235:genetics / Q000276:immunology / Q000378:metabolism*; D017931:DNA Primers / Q000235:genetics; D018076:DNA, Complementary / Q000235:genetics; D006801:Humans; D007249:Inflammation / Q000276:immunology / Q000378:metabolism; D007371:Interferon-gamma / Q000276:immunology / Q000494:pharmacology; D018482:Muscle, Skeletal / Q000166:cytology / Q000276:immunology* / Q000378:metabolism*; D016133:Polymerase Chain Reaction; D011233:Precipitin Tests; D012313:RNA / Q000235:genetics; D012333:RNA, Messenger / Q000378:metabolism
D003175:Complement C2; D003181:Complement C4; D017931:DNA Primers; D018076:DNA, Complementary; D012333:RNA, Messenger; D012313:RNA; D015922:Complement C1q; D007371:Interferon-gamma; D003165:Complement System Proteins; D015923:Complement C1r; D003173:Complement C1s