High-performance liquid chromatography of selenoamino acids and organo selenium compounds. Speciation by inductively coupled plasma mass spectrometry.
As part of an ongoing study to identify selenium compounds with cancer chemopreventive activity, extracts of selenium-enriched samples were analyzed by HPLC-inductively coupled plasma (ICP)-MS. Ion-exchange, ion pair and derivatization methods for reversed-phase HPLC were considered and advantages and disadvantages for each compared. Anion exchange allows separation of selenite and selenate, but otherwise provides poor separation. Pre-column derivatization and reversed-phase chromatography provides separation of compounds with terminal amine functionalities, but many other species elute in the void volume. The ion pair method gave optimal separation and was compatible with standard ICP-MS operating conditions.
Bird
S M
SM
Department of Chemistry, Lederle Graduate Research Tower A, University of Massachusetts, Amherst 01003-4510, USA.
Ge
H
H
Uden
P C
PC
Tyson
J F
JF
Block
E
E
Denoyer
E
E
D000596:Amino Acids / Q000032:analysis; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D002852:Chromatography, Ion Exchange; D013058:Mass Spectrometry / Q000379:methods*; D016566:Organoselenium Compounds / Q000032:analysis*; D018036:Selenium Compounds / Q000032:analysis*
D000596:Amino Acids; D016566:Organoselenium Compounds; D018036:Selenium Compounds