Cloning of a cDNA fragment encoding part of the protein moiety of the 58-kDa fibrinogen-binding mannoprotein of Candida albicans.
Immunoscreening of a Candida albicans expression library with antibodies against the 58 kDa fibrinogen-binding mannoprotein (mp58) of the fungus resulted in the isolation of clones encoding the protein moiety of this molecule. Sequence of the 0.9 kb cDNA of one of the clones selected for further analysis, revealed an open reading frame coding for 292 amino acids, which displays sequence similarity to proteins belonging to a family of immunodominant antigens of Aspergillus spp. The gene corresponding to this cDNA was named FBP1 (fibrinogen-binding protein). These results represent the first report on the identification of C. albicans genes encoding surface receptors for host proteins.
López-Ribot
J L
JL
Department of Medicine, University of Texas Health Sciences Center at San Antonio, TX 78284, USA.
Sepúlveda
P
P
Cervera
A M
AM
Roig
P
P
Gozalbo
D
D
Martínez
J P
JP
D000595:Amino Acid Sequence; D001483:Base Sequence; D015139:Blotting, Southern; D002176:Candida albicans / Q000235:genetics*; D002448:Cell Adhesion; D015815:Cell Adhesion Molecules; D002473:Cell Wall / Q000737:chemistry; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics; D005340:Fibrinogen / Q000378:metabolism; D005656:Fungal Proteins / Q000235:genetics* / Q000378:metabolism; D005800:Genes, Fungal; D008969:Molecular Sequence Data; D008970:Molecular Weight; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid
C109455:ALA1 protein, Candida albicans; D015815:Cell Adhesion Molecules; D018076:DNA, Complementary; D005656:Fungal Proteins; D005340:Fibrinogen