Stability indicating high performance liquid chromatography methods for 5-fluorouridine in aqueous solution.
Two methods of reversed-phase high performance liquid chromatography (RP-HPLC) were used to detect and quantify the degradation of 5-fluorouridine (CAS 316-46-1, 5-FUR) in aqueous solution. The 1H-NMR technique was used for identifying the degradation products. Several assays were performed from different aqueous solutions of 5-FUR. The best results (optimal resolution, reproducibility and rapidity) were achieved by using Resolve C-18 as stationary phase and a mix (96:4) of 50 mmol/l ammonium dihydrogen phosphate (adjusted to pH 3.5 with phosphoric acid) and acetonitrile as mobile phase. Despite the 5-FUR hydrolysis to 5-fluorouracil (5-FU) and D-ribose under moderate conditions in unlikely, due to high stability of the nucleoside, these were the identified degradation products in assays carried out.
Dorta
M J
MJ
Departamento Ingeniería Química y Tecnología Farmacéutica, Facultad de Farmacia, Universidad de La Laguna, Tenerife, Spain.
Munguía
O
O
Fariña
J B
JB
Martin
V S
VS
Llabrés
M
M
D002851:Chromatography, High Pressure Liquid; D004355:Drug Stability; D009682:Magnetic Resonance Spectroscopy; D012996:Solutions; D013056:Spectrophotometry, Ultraviolet; D014529:Uridine / Q000031:analogs & derivatives* / Q000032:analysis
D012996:Solutions; C001943:5-fluorouridine; D014529:Uridine