title: Regulation of NMDA receptor subunit mRNA expression in the guinea pig vestibular nuclei following unilateral labyrinthectomy.
abstract: The localization of neurons expressing mRNAs for the NR1 and NR2A-D subunits of the glutamatergic NMDA receptor was examined by non-radioactive in situ hybridization throughout the guinea pig vestibular nuclei. After deafferentation of the vestibular nuclei by unilateral labyrinthectomy, modifications of the mRNA distributions were followed for 30 days. A quantitative analysis was performed in the medial vestibular nucleus by comparison of the labelled neurons in the ipsi- and contra-lateral nuclei. In vestibular nuclei, the NR1 subunit mRNA was found in various populations of neurons. The NR2A and NR2C subunit mRNAs were less widely distributed, whereas little NR2D mRNA was detected and only rare cells contained NR2B mRNA. NR1 and NR2A-D mRNAs were colocalized in some but not other neuronal types. Twenty hours after the lesion, there was a transient ipsilateral increase of NR1 mRNA level in the medial vestibular nucleus, followed by a decrease 48 h after the lesion and, at 3 days, by recovery to the control level. An ipsilateral increase in the mRNA level of NR2C subunit was detected 20 h after lesion and maintained at 48 h. No significant changes were apparent in NR2A, NR2B and NR2D mRNA levels. The distributions and the differential signal intensities of NR2A-D mRNAs suggest various subunit organizations of the NMDA receptors in different neurons of the vestibular nuclei. Neuronal plasticity reorganizations in the vestibular nuclei following unilateral labyrinthectomy appear to include only changes in NR1 and NR2C mRNA levels modifying the functional diversity of the NMDA receptor in the ipsilateral medial vestibular nucleus neurons. The transient changes in NR1 and the NR2C subunit mRNA expressions in response to sensory deprivation are consistent with an active role for NMDA receptors in the appearance and development of the vestibular compensatory process.
authors.affiliation: INSERM U432, Neurobiologie et Développement du Système Vestibulaire, Université de Montpellier II, France.
authors.forename: N
authors.initials: N
authors.lastname: Sans
authors.forename: A
authors.initials: A
authors.lastname: Sans
authors.forename: J
authors.initials: J
authors.lastname: Raymond
mesh_terms: D000818:Animals; D002525:Cerebellar Cortex / Q000378:metabolism; D002531:Cerebellum / Q000166:cytology / Q000378:metabolism*; D007758:Ear, Inner / Q000502:physiology* / Q000601:surgery; D007839:Functional Laterality; D006168:Guinea Pigs; D008297:Male; D009474:Neurons / Q000145:classification / Q000166:cytology / Q000378:metabolism*; D015345:Oligonucleotide Probes; D011689:Purkinje Cells / Q000166:cytology / Q000378:metabolism; D012333:RNA, Messenger / Q000096:biosynthesis; D051381:Rats; D017208:Rats, Wistar; D016194:Receptors, N-Methyl-D-Aspartate / Q000096:biosynthesis* / Q000737:chemistry; D014158:Transcription, Genetic; D014726:Vestibular Nuclei / Q000378:metabolism*
chemical_list: D015345:Oligonucleotide Probes; D012333:RNA, Messenger; D016194:Receptors, N-Methyl-D-Aspartate