title: Detection of Arcobacter species in gastric samples from swine.
abstract: Swine stomachs were surveyed for evidence of Arcobacter spp. and Helicobacter spp. infections associated with gastric ulceration. A nested PCR test targeted to the 16S rRNA was developed to detect many Arcobacter spp. and Helicobacter spp. An internal oligonucleotide probe was used for differentiation and confirmation of the PCR product. Tissue samples were obtained from the nonglandular and glandular regions of 86 swine stomachs. Evidence of infection with these microbes was detected in 51%, with 77% of the positive samples being identified as A. butzleri using a highly specific probe. Nonglandular stomach samples (44%) were more likely to be positive by PCR than samples from the glandular (23%) region. Gross lesions of any stage of gastric ulceration, ranging from parakeratosis, erosions and ulceration, were observed in 24% of stomachs examined. Of 21 samples with lesions, 52% were positive by the broadly reactive PCR assay for Arcobacter spp. and Helicobacter spp. The majority of PCR-positive samples (75%) had no gross lesions. When a single step PCR assay that was more specific for Arcobacter spp. was used on the nonglandular stomach samples, 10.4% of the 86 samples were positive. Arcobacter spp. were cultured from four of the sample stomachs. Partial sequencing of the 16S rRNA gene identified the isolates as A. butzleri (n = 2), A. cryaerophilus, (n = 1), and a mixed culture of A. butzleri and another Arcobacter spp. (n = 1). A single step PCR assay targeted to the urease gene and culturing methods were used to screen for H. pylori or other closely related urease positive bacteria, but none were found.
authors.affiliation: National Animal Disease Center, USDA/Agricultural Research Service, Ames, IA 50010, USA. dsuarez@asrr.arsusda.gov
authors.forename: D L
authors.initials: DL
authors.lastname: Suarez
authors.forename: I V
authors.initials: IV
authors.lastname: Wesley
authors.forename: D J
authors.initials: DJ
authors.lastname: Larson
mesh_terms: D000818:Animals; D001483:Base Sequence; D017931:DNA Primers; D005753:Gastric Mucosa / Q000382:microbiology*; D006090:Gram-Negative Bacteria / Q000145:classification* / Q000302:isolation & purification*; D016905:Gram-Negative Bacterial Infections / Q000175:diagnosis / Q000662:veterinary*; D016998:Helicobacter / Q000302:isolation & purification; D016481:Helicobacter Infections / Q000175:diagnosis / Q000662:veterinary*; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction / Q000379:methods; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012680:Sensitivity and Specificity; D013270:Stomach / Q000382:microbiology*; D013276:Stomach Ulcer / Q000382:microbiology / Q000662:veterinary*; D013552:Swine; D013553:Swine Diseases
chemical_list: D017931:DNA Primers; D012336:RNA, Ribosomal, 16S