Increase of ciliary neurotrophic factor (CNTF) in the ischemic rat brain as determined by a sensitive enzyme-linked immunoassay.
To study the distribution of ciliary neurotrophic factor (CNTF) in the rat brain under physiological as well as pathological conditions, a sensitive sandwich enzyme linked immunoassay (EIA) for rat CNTF was developed using rabbit anti-CNTF antibody. This method detected as little as 10 pg ml-1 CNTF with a good linearity and accuracy. Analysis of various regions of rat brain and peripheral nerves showed a high content of CNTF in the sciatic nerves, spinal cord, optic nerves, and olfactory bulb. Transient focal ischemia of rat brain caused by middle cerebral artery occlusion significantly and specifically increased CNTF levels in the cerebral cortex and hippocampus regions in the ischemic hemisphere.
Hu
J
J
Department of Neurology, Tohoku University School of Medicine, Sendai, Japan.
Saito
T
T
Abe
K
K
Deguchi
T
T
D000818:Animals; D000906:Antibodies; D001921:Brain / Q000378:metabolism*; D002540:Cerebral Cortex / Q000378:metabolism; D020934:Ciliary Neurotrophic Factor; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D007839:Functional Laterality; D006624:Hippocampus / Q000378:metabolism; D002546:Ischemic Attack, Transient / Q000378:metabolism*; D008297:Male; D009419:Nerve Tissue Proteins / Q000032:analysis / Q000096:biosynthesis*; D009830:Olfactory Bulb / Q000378:metabolism; D009900:Optic Nerve / Q000378:metabolism; D009928:Organ Specificity; D011817:Rabbits; D051381:Rats; D017207:Rats, Sprague-Dawley; D012016:Reference Values; D015203:Reproducibility of Results; D012584:Sciatic Nerve / Q000378:metabolism; D012680:Sensitivity and Specificity; D013116:Spinal Cord / Q000378:metabolism
D000906:Antibodies; D020934:Ciliary Neurotrophic Factor; D009419:Nerve Tissue Proteins