Ammonification in Bacillus subtilis utilizing dissimilatory nitrite reductase is dependent on resDE.
During anaerobic nitrate respiration Bacillus subtilis reduces nitrate via nitrite to ammonia. No denitrification products were observed. B. subtilis wild-type cells and a nitrate reductase mutant grew anaerobically with nitrite as an electron acceptor. Oxygen-sensitive dissimilatory nitrite reductase activity was demonstrated in cell extracts prepared from both strains with benzyl viologen as an electron donor and nitrite as an electron acceptor. The anaerobic expression of the discovered nitrite reductase activity was dependent on the regulatory system encoded by resDE. Mutation of the gene encoding the regulatory Fnr had no negative effect on dissimilatory nitrite reductase formation.
Hoffmann
T
T
Institut für Organische Chemie und Biochemie, Albert-Ludwigs-Universität Freiburg, Germany.
Frankenberg
N
N
Marino
M
M
Jahn
D
D
D000641:Ammonia / Q000378:metabolism*; D000693:Anaerobiosis; D001412:Bacillus subtilis / Q000201:enzymology* / Q000235:genetics; D001426:Bacterial Proteins / Q000235:genetics / Q000502:physiology*; D001594:Benzyl Viologen; D003580:Cytochromes; D004268:DNA-Binding Proteins; D003576:Electron Transport Complex IV / Q000235:genetics / Q000378:metabolism*; D029968:Escherichia coli Proteins; D007202:Indicators and Reagents; D007506:Iron-Sulfur Proteins / Q000502:physiology; D050901:Nitrate Reductase; D009565:Nitrate Reductases / Q000378:metabolism; D009566:Nitrates / Q000378:metabolism*; D009572:Nitrite Reductases / Q000235:genetics / Q000378:metabolism*; D010084:Oxidation-Reduction; D014157:Transcription Factors
D001426:Bacterial Proteins; D003580:Cytochromes; D004268:DNA-Binding Proteins; D029968:Escherichia coli Proteins; C060704:FNR protein, E coli; D007202:Indicators and Reagents; D007506:Iron-Sulfur Proteins; D009566:Nitrates; C100386:ResD protein, Bacillus subtilis; D014157:Transcription Factors; D001594:Benzyl Viologen; D000641:Ammonia; D009565:Nitrate Reductases; D009572:Nitrite Reductases; C028123:nitrite reductase (NO-forming); D050901:Nitrate Reductase; D003576:Electron Transport Complex IV