Selection of a high tanshinone-producing crown gall strain and production of tanshinone in the strain.
Crown galls were induced by direct infection of sterile seedlings of Salvia miltiorrhiza with Agrobacterium tumefaciens C58 and subcultured on a 67-V hormone-free medium by successively selecting red cell aggregates. A high tanshinone-producing crown gall strain C1 was obtained after 12 months in a subculture. It grows well and retains its high tanshinone-producing characteristic in liquid stationary cultures. It is obvious that the yeast extract and fermentation extract of Armillaria mellea as elicitors promote strain C1 to produce tanshinone. Tanshinone content of strain C1 cultures was over three times higher than that of the crude drugs. The results indicated that the crown gall tissue and elicitor technique could provide some new clues for the production of tanshinone under the condition of a liquid stationary culture.
Song
J
J
Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences, Peking Union Medical College, Beijing, China.
Zhang
Y
Y
Qi
J
J
Lu
G
G
D045784:Abietanes; D004365:Drugs, Chinese Herbal; D010616:Phenanthrenes; D010941:Plant Tumors
D045784:Abietanes; D004365:Drugs, Chinese Herbal; D010616:Phenanthrenes; C021751:tanshinone