title: Rapid and simple determination of oxolinic acid and oxytetracycline in the shell of the blue mussel (Mytilus edulis) by high-performance liquid chromatography.
abstract: A simple procedure for the determination of oxolinic acid (OA) and oxytetracycline (OTC), two antibacterial agents, in the shell of the blue mussel (Mytilus edulis), using reversed-phase high-performance liquid chromatography is described. Liquid chromatography was performed on a 5-microm LiChroSpher 100 RP-18E column using acetonitrile and a 0.02 M orthophosphoric acid solution as the mobile phase, with ultraviolet detection. After roughly grinding the shell, drugs were extracted using a methanolic oxalic acid solution. Linearity and precision were checked over the concentration range 0.04-0.32 microg/g. Limits of detection of OA and OTC were 0.012 and 0.008 microg/g, respectively. Mean extraction recoveries of OA and OTC from mussel shell were 72.9 and 65.4%, respectively. To demonstrate the usefulness of the analytical procedure, an experimental study was performed in blue mussels exposed to the drugs for eight days.
authors.affiliation: Ecole Nationale Vétérinaire de Nantes, Laboratoire de Chimiothérapie Aquacole et Environnement, Atlanpôle, La Chantrerie, France.
authors.forename: H
authors.initials: H
authors.lastname: Pouliquen
authors.forename: D
authors.initials: D
authors.lastname: Gouelo
authors.forename: M
authors.initials: M
authors.lastname: Larhantec
authors.forename: N
authors.initials: N
authors.lastname: Pilet
authors.forename: L
authors.initials: L
authors.lastname: Pinault
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000032:analysis*; D000890:Anti-Infective Agents / Q000032:analysis*; D049872:Bivalvia / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D002940:Circadian Rhythm; D004350:Drug Residues / Q000032:analysis; D005506:Food Contamination / Q000032:analysis; D016014:Linear Models; D009994:Osmolar Concentration; D010093:Oxolinic Acid / Q000032:analysis*; D010118:Oxytetracycline / Q000032:analysis*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D012758:Shellfish / Q000032:analysis*
chemical_list: D000900:Anti-Bacterial Agents; D000890:Anti-Infective Agents; D010093:Oxolinic Acid; D010118:Oxytetracycline