title: Immunogenetics of the A-E alloantigen complex.
abstract: The close linkage (0.5%) between the A and E erythrocyte alloantigen loci present a special challenge in the production of locus-specific typing antisera. The objective of the investigation was to determine immunogenetically the A-E haplotypes (genetically linked combinations of A and E antigens) existing in the locally maintained individuals of the New Hampshire (NH) and White Plymouth Rock (WR) breeds. The A and E alloantigens in these populations were identified using reference antisera previously produced in White Leghorns. A total of four A-E haplotypes were identified within each of the two breeds; A2E1, A6E2, A6E4, and A8E2 in WR and A2E1, A3E7, A7E4, and A8E2 in NH. Individuals of these two brown-egg breeds were backcrossed over several generations to a line of Ancona chickens homozygous at the A and E loci. Genetic segregation occurring over four generations resulted in nonrecombinant and recombinant progeny that were immunized reciprocally with the blood of siblings to raise antibodies reactive with the individual A and E antigens of the NH and WR stocks. The antisera resulting from the within-family alloimmunizations confirmed the haplotypes deduced in the WR and NH lines from the initial tests with the A and E reference antisera.
authors.affiliation: Department of Biological Sciences, Northern Illinois University, DeKalb 60115, USA.
authors.forename: F
authors.initials: F
authors.lastname: Kopti
authors.forename: R W
authors.initials: RW
authors.lastname: Briles
authors.forename: W E
authors.initials: WE
authors.lastname: Briles
mesh_terms: D000818:Animals; D000918:Antibody Specificity; D000936:Antigen-Antibody Complex / Q000235:genetics*; D001789:Blood Group Antigens / Q000235:genetics* / Q000276:immunology*; D001947:Breeding; D002645:Chickens / Q000097:blood / Q000235:genetics* / Q000276:immunology*; D004912:Erythrocytes / Q000276:immunology*; D005260:Female; D005838:Genotype; D006239:Haplotypes; D006386:Hemagglutination Tests / Q000379:methods / Q000662:veterinary; D006720:Homozygote; D007106:Immune Sera / Q000276:immunology; D007519:Isoantigens / Q000097:blood / Q000235:genetics*; D008297:Male
chemical_list: D000936:Antigen-Antibody Complex; D001789:Blood Group Antigens; D007106:Immune Sera; D007519:Isoantigens