title: Actin: a target of lipopolysaccharide-induced phosphorylation in human monocytes.
abstract: We have previously reported that lipopolysaccharide (LPS) causes altered phosphate labelling of cytosolic proteins of 36 kDa and 38 kDa (p36/38) and that inhibition of phosphorylation is accompanied by a loss of cytokine production. Here we have purified the two phosphorylated proteins via two-dimensional polyacrylamide gel electrophoresis. P 36 was found to consist of two proteins p36a and p36b. The proteins were analysed by matrix-assisted laser desorption ionization (MALDI) mass spectrometry and p36b was identified as gamma-actin, p36a as beta/gamma-actin. The ability of LPS to cause altered phosphate labelling of beta/gamma-actin suggests a participation of the microfilament network in LPS-induced monocyte activation.
authors.affiliation: Department of Immunobiology, University of Leipzig, Germany.
authors.forename: S
authors.initials: S
authors.lastname: Hauschildt
authors.forename: C
authors.initials: C
authors.lastname: Schwarz
authors.forename: H
authors.initials: H
authors.lastname: Heine
authors.forename: A J
authors.initials: AJ
authors.lastname: Ulmer
authors.forename: H D
authors.initials: HD
authors.lastname: Flad
authors.forename: E T
authors.initials: ET
authors.lastname: Rietschel
authors.forename: O N
authors.initials: ON
authors.lastname: Jensen
authors.forename: M
authors.initials: M
authors.lastname: Mann
mesh_terms: D000199:Actins / Q000302:isolation & purification / Q000378:metabolism*; D000595:Amino Acid Sequence; D003600:Cytosol / Q000378:metabolism; D006801:Humans; D007536:Isomerism; D008070:Lipopolysaccharides / Q000494:pharmacology*; D008262:Macrophage Activation; D008969:Molecular Sequence Data; D008970:Molecular Weight; D009000:Monocytes / Q000187:drug effects / Q000378:metabolism*; D010766:Phosphorylation / Q000187:drug effects; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
chemical_list: D000199:Actins; D008070:Lipopolysaccharides