Inhibition of HIV replication by capsid mutant C6b.
A Gag capsid mutant of human immunodeficiency virus type 1 (HIV-1) designated C6b was biologically and biochemically characterized with respect to its ability to suppress the replication of wild-type (wt) HIV. The C6b efficiently interfered with the replication of wt HIV-1 in the cleavage of Gag precursor, and also in the early replication process before or during viral DNA synthesis after viral penetration. The C6b Gag appeared to be unable to form chimeric multimers with HIV-2 Gag and failed to inhibit the replication of wt HIV-2.
Shimano
R
R
Department of Virology, School of Medicine, University of Tokushima, Japan.
Iida
S
S
Fukumori
T
T
Yamamoto
Y
Y
Kawamura
M
M
Furuta
R A
RA
Adachi
A
A
D015139:Blotting, Southern; D015153:Blotting, Western; D002460:Cell Line; D015500:Chloramphenicol O-Acetyltransferase / Q000235:genetics / Q000378:metabolism; D004279:DNA, Viral / Q000096:biosynthesis; D015683:Gene Products, gag / Q000235:genetics / Q000378:metabolism*; D017930:Genes, Reporter; D015750:Genes, gag / Q000235:genetics*; D015497:HIV-1 / Q000235:genetics / Q000378:metabolism*; D015498:HIV-2 / Q000378:metabolism; D006801:Humans; D009154:Mutation / Q000235:genetics; D014162:Transfection / Q000235:genetics; D014779:Virus Replication / Q000235:genetics*
D004279:DNA, Viral; D015683:Gene Products, gag; D015500:Chloramphenicol O-Acetyltransferase