title: Lack of colocalization of HBxAg and insulin like growth factor II in the livers of patients with chronic hepatitis B, cirrhosis and hepatocellular carcinoma.
abstract: To evaluate the possibility that HBxAg is related to an enhanced expression of IGF-II, immunohistochemical staining was performed for distribution and colocalization of HBxAg and IGF-II in liver tissues from 40 chronic active hepatitis (CAH-B), 51 cirrhosis and 46 hepatocellular carcinoma (HCC) patients using polyclonal rabbit anti HBxAg raised against full length-recombinant HBxAg and monoclonal mouse anti IGF-II. HBxAg in CAH-B, cirrhosis and HCC tissues was detected in 95%, 39% and 17%, whereas IGF-II in the same tissues was seen in 0%, 92% and 100%, respectively. There was a gradual decrease in the prevalence of HBxAg expression in cirrhosis and HCC, as compared to CAH-B tissues. All of the cirrhosis and HCC samples with positive staining for HBxAg expressed IGF-II. However, 55% of cirrhosis and 100% of HCC samples without HBxAg staining also expressed IGF-II. Moreover, colocalization at neighboring sections, even in both HBxAg and IGF-II positive samples, was not regularly observed. It is concluded that HBxAg expression in CAH-B may play a role in the pathogenesis of CAH-B. Although HBxAg may be related to the expression of IGF-II in some cirrhotic and HCC tissues, IGF-II expression in a large majority of these cases may be related to other factor(s) than HBxAg.
authors.affiliation: Department of Molecular Biology, Pusan National University, Korea.
authors.forename: J H
authors.initials: JH
authors.lastname: Seo
authors.forename: K W
authors.initials: KW
authors.lastname: Kim
authors.forename: S
authors.initials: S
authors.lastname: Murakami
authors.forename: B C
authors.initials: BC
authors.lastname: Park
mesh_terms: D000328:Adult; D000911:Antibodies, Monoclonal / Q000276:immunology; D000918:Antibody Specificity; D006528:Carcinoma, Hepatocellular / Q000378:metabolism*; D005260:Female; D006511:Hepatitis B Antigens / Q000032:analysis; D019694:Hepatitis B, Chronic / Q000378:metabolism*; D006801:Humans; D007074:Immunoglobulin G / Q000276:immunology; D007150:Immunohistochemistry; D007335:Insulin-Like Growth Factor II / Q000032:analysis* / Q000096:biosynthesis; D008099:Liver / Q000737:chemistry / Q000276:immunology / Q000473:pathology*; D008103:Liver Cirrhosis / Q000378:metabolism*; D008113:Liver Neoplasms / Q000378:metabolism*; D008297:Male; D008875:Middle Aged; D015534:Trans-Activators / Q000032:analysis*; D054334:Viral Regulatory and Accessory Proteins
chemical_list: D000911:Antibodies, Monoclonal; D006511:Hepatitis B Antigens; D007074:Immunoglobulin G; D015534:Trans-Activators; D054334:Viral Regulatory and Accessory Proteins; C057628:hepatitis B virus X protein; D007335:Insulin-Like Growth Factor II