title: Cytokine gene transcription in simian immunodeficiency virus and human immunodeficiency virus-associated non-Hodgkin lymphomas.
abstract: Infection of rhesus monkeys with SIV leads to AIDS-like symptoms. Similar to human AIDS patients, some monkeys develop B cell non-Hodgkin lymphoma (NHL). We determined transcription of cytokine genes regulating the activation of B and T cells, which play a role in intratumoral immune surveillance. Therefore, we compared the transcription of the cytokine genes encoding IL-2, IL-4, IL-6, IL-10, IFN-gamma, TNF-alpha, and TGF-beta1, and the Epstein-Barr virus-encoded BCRF 1 gene, in cells from five monkey and two human tumor specimens. The immune-suppressive IL-10 and TGF-beta1 genes were predominantly transcribed in all tumor specimens analyzed. Cytokine gene transcription patterns appeared to be similar in human and animal tumor cells. The transcription patterns corresponded to their histological classification as diffuse large-cell lymphoma according to the REAL classification and as immunoblastic or centroblastic tumors according to the Kiel classification. The determination of cytokine gene transcription pattern in the NHL may improve our understanding of pathogenesis and immune surveillance in this heterogeneous group of tumors. Our data show that SIV-associated NHLs of rhesus monkeys are comparable to human HIV-1-associated EBV-positive non-Hodgkin lymphoma.
authors.affiliation: German Primate Centre, Göttingen.
authors.forename: H
authors.initials: H
authors.lastname: Hannig
authors.forename: K
authors.initials: K
authors.lastname: Mätz-Rensing
authors.forename: E M
authors.initials: EM
authors.lastname: Kuhn
authors.forename: C
authors.initials: C
authors.lastname: Stahl-Hennig
authors.forename: F J
authors.initials: FJ
authors.lastname: Kaup
authors.forename: G
authors.initials: G
authors.lastname: Hunsmann
authors.forename: W
authors.initials: W
authors.lastname: Bodemer
mesh_terms: D000818:Animals; D002461:Cell Line, Transformed; D016207:Cytokines / Q000096:biosynthesis / Q000235:genetics*; D015658:HIV Infections / Q000150:complications* / Q000276:immunology; D004854:Herpesvirus 4, Human / Q000235:genetics; D006801:Humans; D016753:Interleukin-10; D016483:Lymphoma, AIDS-Related / Q000276:immunology / Q000378:metabolism*; D008228:Lymphoma, Non-Hodgkin / Q000150:complications / Q000276:immunology / Q000378:metabolism*; D008253:Macaca mulatta; D016133:Polymerase Chain Reaction / Q000379:methods / Q000592:standards; D016097:Simian Acquired Immunodeficiency Syndrome / Q000150:complications* / Q000276:immunology; D014158:Transcription, Genetic; D014764:Viral Proteins / Q000235:genetics
chemical_list: C066460:BCRF1 protein, Human herpesvirus 4; D016207:Cytokines; D014764:Viral Proteins; D016753:Interleukin-10