title: Bronchial responsiveness and airway inflammation in patients with nonallergic rhinitis with eosinophilia syndrome.
abstract: BACKGROUND
  Nonallergic rhinitis with eosinophilia syndrome (NARES) is characterized by persistent nasal symptoms without allergy and by a marked eosinophil recruitment in the nasal cavities.
  
  
  OBJECTIVE
  We studied whether patients with NARES had bronchial responsiveness to methacholine and airway inflammation and examined the relationship between these factors.
  
  
  METHODS
  We selected a group of 39 patients referred to our allergy clinic for symptoms of perennial rhinitis. Atopic status was excluded by skin prick tests and RASTs. None of the patients had a history of respiratory symptoms. We preliminarily performed nasal lavage in all patients, and the diagnosis of NARES was made on the basis of the presence of at least 10% eosinophils in nasal lavage fluid. A methacholine challenge and sputum induction were also done on two different days.
  
  
  RESULTS
  Eosinophils in nasal lavage fluid ranged between 10% and 86%. Serum IgE levels were within normal range. Total circulating eosinophils ranged between 40 and 890/mm3. Methacholine PD20 values were measurable in only 18 patients (range, 0.32 to 22.56 micromol; group 1). In the remaining 21 patients, methacholine PD20 values were greater than 24 micromol (group 2). We found that differential cell counts in nasal lavage fluid in group 1 were not different from those in group 2. Methacholine PD20 values were not significantly related to any cell count in the nasal lavage fluid. Induced sputum was accomplished only in 22 patients. Eosinophils in induced sputum ranged between 0% and 56.5%. Numbers of total cells, macrophages, lymphocytes, neutrophils, and epithelial cells in the two subgroups were not different. The number of metachromatic cells tended to be higher in group 1 compared with group 2 (0.31% vs 0.05%), but the difference was not significant. The eosinophil count in the induced sputum was significantly higher in group 1 compared with group 2 (16.8% vs 3.1%; p < 0.05). In the entire population, methacholine PD20 values were significantly correlated with the number of eosinophils in sputum (r = -0.63; p < 0.001).
  
  
  CONCLUSIONS
  We showed that 46% of patients with NARES but without histories of respiratory symptoms had a measurable bronchial responsiveness. The presence of bronchial responsiveness was associated with an increased number of eosinophils in induced sputum but not with the inflammatory process in the nose.
authors.affiliation: Servizio di Fisiopatologia Respiratoria, Modulo di Allergologia ed Immunopatologia Polmonare, Ospedale di Sesto San Giovanni, Italy.
authors.forename: C
authors.initials: C
authors.lastname: Leone
authors.forename: C
authors.initials: C
authors.lastname: Teodoro
authors.forename: A
authors.initials: A
authors.lastname: Pelucchi
authors.forename: B
authors.initials: B
authors.lastname: Mastropasqua
authors.forename: G
authors.initials: G
authors.lastname: Cavigioli
authors.forename: L
authors.initials: L
authors.lastname: Marazzini
authors.forename: A
authors.initials: A
authors.lastname: Foresi
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D001772:Blood Cell Count; D001980:Bronchi / Q000473:pathology* / Q000503:physiopathology; D016535:Bronchial Hyperreactivity / Q000097:blood / Q000473:pathology* / Q000503:physiopathology; D002452:Cell Count; D004802:Eosinophilia / Q000097:blood / Q000473:pathology* / Q000503:physiopathology; D005260:Female; D006801:Humans; D007249:Inflammation / Q000097:blood / Q000473:pathology / Q000503:physiopathology; D008297:Male; D008875:Middle Aged; D017817:Nasal Lavage Fluid / Q000166:cytology; D012220:Rhinitis / Q000097:blood / Q000473:pathology* / Q000503:physiopathology; D013183:Sputum / Q000166:cytology; D013577:Syndrome