Adhesion of coagulase-negative staphylococci grouped according to physico-chemical surface properties.
Physico-chemical cell surface properties of 23 coagulase-negative staphylococcal strains, including contact angles, zeta potentials and elemental cell surface composition were measured, together with the adhesion of all strains to hexadecane. The data were employed in a hierarchical cluster analysis, revealing that the 23 strains comprised essentially four different groups. Groups I-III were somewhat similar to each other, but group IV was markedly distinguished from the other strains, predominantly through an elevated acidity of the cell surface. These group distinctions were not related to the presence of a capsule or slime on the strains. Adhesion of the strains to hexadecane depended critically on electrostatic interactions between the hexadecane and the staphylococci, and adhesion only occurred when the electrostatic repulsion between hexadecane and the micro-organisms was less than 500 kT at closest approach. Adhesion of six representative strains from all four groups in a parallel plate flow chamber to silicone rubber, an implant material with similar hydrophobicity to hexadecane, did not show such a critical dependence, nor did it relate with the group distinction. Possibly, microbial adhesion to substratum surfaces like silicone rubber is more complicated than adhesion to an ideally smooth and homogeneous hexadecane surface in an aqueous solution. Adhesion of all six strains to silicone rubber with an adsorbed conditioning film of plasma proteins was less than that to bare silicone rubber: initial deposition rates dropped from 2000-3000 cm-2 s-1 to 100-300 cm-2 s-1 after adsorption of plasma proteins, while the stationary end-point adhesion decreased from 10 x 10(6)-15 x 10(6) cm-2 to 1 x 10(6)-5 x 10(6) cm-2. The adhering staphylococci poorly withstood the passage of an air-bubble through the parallel plate flow chamber, regardless of the presence of a conditioning film, indicating a low affinity of these relatively hydrophilic strains for hydrophobic substratum surfaces.
van der Mei
Henny C
HC
Laboratory for Materia Technica, University of Groningen,Bioemsingel 10, 9712 KZ Groningen,The Netherlands.
van de Belt-Gritter
Betsy
B
Laboratory for Materia Technica, University of Groningen,Bioemsingel 10, 9712 KZ Groningen,The Netherlands.
Reid
Gregor
G
Department of Microbiology and Immunology, University of Western Ontario, Health Sciences Centre,London, Ontario,Canada N6A 5C1.|Laboratory for Materia Technica, University of Groningen,Bioemsingel 10, 9712 KZ Groningen,The Netherlands.
Bialkowska-Hobrzanska
Hanna
H
Lawson Research Institute,268 Grosvenor Street, London, Ontario,Canada N6A 4V2.
Busscher
Henk J
HJ
Department of Microbiology and Immunology, University of Western Ontario, Health Sciences Centre,London, Ontario,Canada N6A 5C1.|Laboratory for Materia Technica, University of Groningen,Bioemsingel 10, 9712 KZ Groningen,The Netherlands.
D000473:Alkanes; D001422:Bacterial Adhesion; D015373:Bacterial Typing Techniques; D002462:Cell Membrane / Q000502:physiology; D016000:Cluster Analysis; D003030:Coagulase / Q000032:analysis*; D010802:Phylogeny; D012150:Polymorphism, Restriction Fragment Length; D013212:Staphylococcus epidermidis / Q000737:chemistry / Q000145:classification* / Q000502:physiology*; D055672:Static Electricity; D013499:Surface Properties
D000473:Alkanes; D003030:Coagulase; C007932:n-hexadecane