Interactions of gonadal steroids and pesticides (DDT, DDE) on gonaduct growth in larval tiger salamanders, Ambystoma tigrinum.
In view of the current worldwide decline in amphibian populations, exploratory studies are needed to assess the potential for environmental contaminants to act as endocrine disrupters of the amphibian reproductive system. The present study investigated the effects of DDT dichlorodiphenyltrichloroethane (DDT) and dichlorodiphenyldichloroethylene (DDE) on the development of amphibian gonaducts. Larval male and female tiger salamanders (Ambystoma tigrinum), with immature gonads, were immersed in a sublethal solution of p,p'-DDE or technical-grade DDT (80% p,p'-DDT and 20% o,p'-DDT). Additionally, larvae were injected with the steroid hormones estradiol or dihydrotestosterone (DHT). Morphometrics were used to analyze the effects and interactions of steroid and pesticide treatments on larval gonaducts. Estradiol and DHT stimulated cell proliferation and hypertrophy of the müllerian duct epithelium in both sexes. Wolffian duct epithelium, however, was stimulated only by DHT treatment. The pesticide DDT antagonized the estrogenic actions of the steroid treatments, and p,p'-DDE acted as an estrogen on the müllerian ducts of females only. The müllerian ducts of males, and the wolffian ducts of both sexes, were unaffected by DDT or DDE alone. While confirming the previously reported estrogenic actions of estradiol and DHT on urodelean gonaducts, the results contradict the expected estrogenic actions of DDT and antiandrogenic actions of p,p'-DDE. Instead, in A. tigrinum, technical-grade DDT had an antiestrogenic action and p,p'-DDE an estrogenic action.
Clark
E J
EJ
Department of Environmental, Population, and Organismic Biology, University of Colorado, Boulder 80309-0334, USA.
Norris
D O
DO
Jones
R E
RE
D000818:Animals; D015331:Cohort Studies; D003634:DDT / Q000008:administration & dosage / Q000378:metabolism / Q000633:toxicity; D003633:Dichlorodiphenyl Dichloroethylene / Q000008:administration & dosage / Q000378:metabolism / Q000633:toxicity; D013196:Dihydrotestosterone / Q000008:administration & dosage / Q000494:pharmacology*; D004848:Epithelium / Q000187:drug effects; D004958:Estradiol / Q000008:administration & dosage / Q000494:pharmacology*; D005260:Female; D007101:Immersion; D007274:Injections, Intraperitoneal / Q000662:veterinary; D007306:Insecticides / Q000008:administration & dosage / Q000378:metabolism / Q000633:toxicity*; D007814:Larva / Q000254:growth & development; D008297:Male; D009095:Mullerian Ducts / Q000033:anatomy & histology / Q000187:drug effects*; D014562:Caudata / Q000196:embryology* / Q000502:physiology; D014928:Wolffian Ducts / Q000033:anatomy & histology / Q000187:drug effects*
D007306:Insecticides; D013196:Dihydrotestosterone; D003633:Dichlorodiphenyl Dichloroethylene; D004958:Estradiol; D003634:DDT