Soluble E-selectin enhances intercellular adhesion molecule-1 (ICAM-1) expression in human tumor cell lines.
E-selectin mediates neovascularization via its soluble form, while its membrane-bound form initiates binding of tumor cells to vascular endothelium. Therefore, it was studied whether soluble E-selectin regulates further adhesion molecules on tumor cells. In tumor cells but not in related nonmalignant cells, intercellular adhesion molecule (ICAM)-1 expression was strikingly increased from 5 to 68% positive cells by in vitro inoculation of a recombinant E-selectin-IgG1 within 24 h, as analyzed by flow cytometry. The absence of changes in the expression of vascular cell adhesion molecule, integrin ligands (CD11a, CD18, integrin alpha 4), and sialyl-Lewis X indicates a specific effect of soluble E-selectin on ICAM-1. A cell adhesion assay revealed that the enhanced adhesion on T-cells to tumor cells mediated by soluble E-selectin-induced ICAM-1 expression was at a maximum after a 12-h incubation period. Therefore, ICAM-1 regulation on tumor cells might be a mechanism of immune escape.
Wittig
B M
BM
First Department of Internal Medicine, Johannes Gutenberg-University of Mainz, Germany.
Treichel
U
U
Blaheta
R
R
Schreiter
T
T
Schwarting
A
A
Meyer zum Büschenfelde
K H
KH
Mayet
W
W
D002448:Cell Adhesion; D019040:E-Selectin / Q000737:chemistry / Q000494:pharmacology*; D015870:Gene Expression; D006801:Humans; D018799:Intercellular Adhesion Molecule-1 / Q000378:metabolism*; D009361:Neoplasm Invasiveness; D012333:RNA, Messenger / Q000235:genetics; D012995:Solubility; D013601:T-Lymphocytes / Q000166:cytology; D013997:Time Factors; D014407:Tumor Cells, Cultured / Q000166:cytology* / Q000276:immunology; D015854:Up-Regulation
D019040:E-Selectin; D012333:RNA, Messenger; D018799:Intercellular Adhesion Molecule-1