Separation of monoclonal antibodies from cell-culture supernatants and ascites fluid using thiophilic agarose.
A one-step purification procedure will yield monoclonal antibodies from cell-culture supernatants and ascites fluids. The chromatographic adsorbent is thiophilic argose, i.e., beaded agarose gel coupled with ligands of thiophilic nature, often with a sulfone group and a sulfur atom. The chromatographic procedure is simply adsorption, wash, elution. The procedure is simple, efficient, and inexpensive.
Bøg-Hansen
T C
TC
Protein Laboratory, Panum Institute, University of Copenhagen, Denmark. tcbh@biobase.dk
D000911:Antibodies, Monoclonal / Q000302:isolation & purification*; D001202:Ascitic Fluid; D002478:Cells, Cultured; D003470:Culture Media; D012685:Sepharose
D000911:Antibodies, Monoclonal; D003470:Culture Media; D012685:Sepharose