Hepatocyte growth factor in glycerol-induced acute renal failure.
Hepatocyte growth factor (HGF) facilitates the regeneration of injured kidney in acute renal failure (ARF). Here we investigated the HGF production in glycerol-induced ARF rats. HGF mRNA expression levels were elevated in liver, spleen, and lung 6-24 h after glycerol injection. Tissue HGF protein levels determined by an enzyme-linked immunosorbent assay also increased in liver and spleen, whereas they decreased in the injured kidney 24 h after injection. Immunohistochemical studies showed that the number of HGF-producing cells did not increase in the liver. HGF receptor/c-Met mRNA levels were elevated only in the kidney. These results indicate that HGF supplied in an endocrine manner may play an important role in the regenerating process following ARF.
Goto
T
T
Department of Urology, Osaka City University Medical School, Osaka, Japan.
Sugimura
K
K
Harimoto
K
K
Kasai
S
S
Kim
T
T
Kishimoto
T
T
D058186:Acute Kidney Injury / Q000139:chemically induced / Q000503:physiopathology*; D000818:Animals; D001806:Blood Urea Nitrogen; D015152:Blotting, Northern; D003404:Creatinine / Q000097:blood; D005990:Glycerol / Q000633:toxicity*; D017228:Hepatocyte Growth Factor / Q000096:biosynthesis*; D007150:Immunohistochemistry; D007668:Kidney / Q000187:drug effects / Q000378:metabolism; D008297:Male; D012333:RNA, Messenger / Q000096:biosynthesis; D051381:Rats; D017207:Rats, Sprague-Dawley
D012333:RNA, Messenger; D017228:Hepatocyte Growth Factor; D003404:Creatinine; D005990:Glycerol