Construction of a panel of canine-rodent hybrid cell lines for use in partitioning of the canine genome.
We have constructed a collection of canine-rodent microcell hybrid cell lines by fusion of canine fibroblast microcell donors with immortalized rodent recipient cells. Characterization of the hybrid cell lines using a combination of fluorescence in situ hybridization and PCR analysis of canine microsatellite repeat sequences allowed selection of a panel of hybrids in which most canine chromosomes are represented. Approximately 90% of genetic markers and genes that were tested could be assigned to 1 of 31 anonymous canine chromosome groups, based on common patterns of retention in the hybrid set. Many of these putative chromosome groups have now been validated by linkage analysis. This panel of cell lines provides a tool for development of genetic, physical, and comparative maps of the canine genome.
Langston
A A
AA
Division of Clinical Research, Fred Hutchinson Cancer Research Center, Seattle, Washington 98104, USA.
Mellersh
C S
CS
Neal
C L
CL
Ray
K
K
Acland
G M
GM
Gibbs
M
M
Aguirre
G D
GD
Fournier
R E
RE
Ostrander
E A
EA
D000818:Animals; D002460:Cell Line; D002874:Chromosome Mapping; D006224:Cricetinae; D003412:Cricetulus; D004285:Dogs / Q000235:genetics*; D005347:Fibroblasts / Q000166:cytology; D016678:Genome; D006822:Hybrid Cells; D017404:In Situ Hybridization, Fluorescence; D008297:Male; D051379:Mice; D018895:Microsatellite Repeats; D016133:Polymerase Chain Reaction