title: Neuronal cell surface molecules mediate specific binding to rabies virus glycoprotein expressed by a recombinant baculovirus on the surfaces of lepidopteran cells.
abstract: The existence of specific rabies virus (RV) glycoprotein (G) binding sites on the surfaces of neuroblastoma cells is demonstrated. Spodoptera frugiperda (Sf21) cells expressing G of the RV strain CVS (Gcvs-Sf21 cells) bind specifically to neuroblastoma cells of different species but not to any other cell type (fibroblast, myoblast, epithelial, or glioma). Attachment to mouse neuroblastoma NG108-15 cells is abolished by previous treatment of Gcvs-Sf2 cells with anti-G antibody. Substitutions for lysine at position 330 and for arginine at position 333 in RV G greatly reduce interaction between Gcvs-Sf21 cells and NG108-15 cells. These data are consistent with in vivo results: an avirulent RV mutant bearing the same double mutation is not able to infect sensory neurons or motoneurons (P. Coulon, J.-P. Ternaux, A. Flamand, and C. Tuffereau, J. Virol. 72:273-278, 1998) after intramuscular inoculation into a mouse. Furthermore, infection of NG108-15 cells by RV but not by vesicular stomatitis virus leads to a reduction of the number of binding sites at the neuronal-cell surface. Our data strongly suggest that these specific attachment sites on neuroblastoma cells represent a neuronal receptor(s) used by RV to infect certain types of neurons in vivo.
authors.affiliation: Laboratoire de Génétique des Virus, CNRS, 91198 Gif sur Yvette, France. ctuffer@gv.cnrs-gif.fr
authors.forename: C
authors.initials: C
authors.lastname: Tuffereau
authors.forename: J
authors.initials: J
authors.lastname: Benejean
authors.forename: A M
authors.initials: AM
authors.lastname: Alfonso
authors.forename: A
authors.initials: A
authors.lastname: Flamand
authors.forename: M C
authors.initials: MC
authors.lastname: Fishman
mesh_terms: D000818:Animals; D016367:Baculoviridae / Q000235:genetics*; D001665:Binding Sites / Q000235:genetics; D002460:Cell Line; D004274:DNA, Recombinant; D006023:Glycoproteins / Q000235:genetics / Q000378:metabolism*; D051379:Mice; D009419:Nerve Tissue Proteins / Q000378:metabolism*; D009447:Neuroblastoma; D009474:Neurons / Q000378:metabolism* / Q000821:virology; D011485:Protein Binding; D011820:Rabies virus / Q000235:genetics / Q000378:metabolism*; D011956:Receptors, Cell Surface / Q000378:metabolism; D011991:Receptors, Virus / Q000378:metabolism*; D018411:Spodoptera; D014764:Viral Proteins / Q000235:genetics / Q000378:metabolism*
chemical_list: D004274:DNA, Recombinant; D006023:Glycoproteins; D009419:Nerve Tissue Proteins; D011956:Receptors, Cell Surface; D011991:Receptors, Virus; D014764:Viral Proteins