Aggregation and binding substances enhance pathogenicity in rabbit models of Enterococcus faecalis endocarditis.
We investigated the importance of enterococcal aggregation substance (AS) and enterococcal binding substance (EBS) in rabbit models of Enterococcus faecalis cardiac infections. First, American Dutch belted rabbits were injected intraventricularly with 10(8) CFU and observed for 2 days. No clinical signs of illness developed in animals given AS- EBS- organisms, and all survived. All rabbits given AS- EBS+ organisms developed signs of illness, including significant pericardial inflammation, but only one of six died. All animals given AS+ EBS- organisms developed signs of illness, including pericardial inflammation, and survived. All rabbits given AS+ EBS+ organisms developed signs of illness and died. None of the rabbits receiving AS+ EBS+ organisms showed gross pericardial inflammation. The lethality and lack of inflammation are consistent with the presence of a superantigen. Rabbit and human lymphocytes were highly stimulated in vitro by cell extracts, but not cell-free culture fluids, of AS+ EBS+ organisms. In contrast, cell extracts from AS- EBS- organisms weakly stimulated lymphocyte proliferation. Culture fluids from human lymphocytes stimulated with AS+/EBS+ enterococci contained high levels of gamma interferon and tumor necrosis factor alpha (TNF-alpha) and TNF-beta, which is consistent with functional stimulation of T-lymphocyte proliferation and macrophage activation. Subsequent experiments examined the abilities of the same strains to cause endocarditis in a catheterization model. New Zealand White rabbits underwent transaortic catheterization for 2 h, at which time catheters were removed and animals were injected with 2 x 10(9) CFU of test organisms. None of the animals given AS- EBS- organisms developed vegetations or showed autopsy evidence of tissue damage. Rabbits given AS- EBS+ or AS+ EBS- organisms developed small vegetations and had splenomegaly at autopsy. All rabbits given AS+ EBS+ organisms developed large vegetations and had splenomegaly and lung congestion at autopsy. Similar experiments that left catheters in place for 3 days revealed that all rabbits given AS- EBS- or AS+ EBS+ organisms developed vegetations, but animals given AS+ EBS+ organisms had larger vegetations and autopsy evidence of lung congestion. These experiments provide direct evidence that these two cell wall components play an important role in the pathogenesis of endocarditis as well as in conjugative plasmid transfer.
Schlievert
P M
PM
Department of Microbiology, University of Minnesota Medical School, Minneapolis 55455-0312, USA. pats@lenti.med.umn.edu
Gahr
P J
PJ
Assimacopoulos
A P
AP
Dinges
M M
MM
Stoehr
J A
JA
Harmala
J W
JW
Hirt
H
H
Dunny
G M
GM
D018829:Adhesins, Bacterial / Q000235:genetics* / Q000378:metabolism* / Q000502:physiology; D000818:Animals; D001422:Bacterial Adhesion; D001426:Bacterial Proteins / Q000235:genetics; D002404:Catheterization / Q000009:adverse effects; D002455:Cell Division; D002457:Cell Extracts / Q000276:immunology / Q000494:pharmacology; D002478:Cells, Cultured; D017077:Culture Media, Conditioned / Q000494:pharmacology; D004697:Endocarditis, Bacterial / Q000276:immunology / Q000378:metabolism*; D013293:Enterococcus faecalis / Q000235:genetics / Q000378:metabolism* / Q000472:pathogenicity*; D006801:Humans; D007249:Inflammation; D007371:Interferon-gamma / Q000378:metabolism; D007963:Leukocytes, Mononuclear / Q000166:cytology / Q000378:metabolism / Q000382:microbiology; D008168:Lung / Q000473:pathology; D008213:Lymphocyte Activation; D008214:Lymphocytes / Q000166:cytology / Q000378:metabolism / Q000382:microbiology; D008233:Lymphotoxin-alpha / Q000378:metabolism; D008262:Macrophage Activation; D011817:Rabbits; D012724:Sex Attractants / Q000235:genetics; D013163:Splenomegaly; D018089:Superantigens / Q000235:genetics / Q000276:immunology; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism
D018829:Adhesins, Bacterial; D001426:Bacterial Proteins; D002457:Cell Extracts; D017077:Culture Media, Conditioned; D008233:Lymphotoxin-alpha; D012724:Sex Attractants; D018089:Superantigens; D014409:Tumor Necrosis Factor-alpha; C065937:aggregation substance, Enterococcus faecalis; D007371:Interferon-gamma