Detection of exogenous hydrocortisone in horse urine by gas chromatography-combustion-carbon isotope ratio mass spectrometry.
A gas chromatography-combustion-isotope ratio mass spectrometry method for confirmation of hydrocortisone abuse in horseracing and equine sports is proposed. Urinary hydrocortisone was converted to a bismethylenedioxy derivative which presents good gas chromatographic properties and brings an extra carbon contribution of only two carbon atoms. Synthetic hydrocortisone has a different 13C abundance from that of natural urinary horse hydrocortisone and the difference is significant, therefore exogenous and endogenous hydrocortisone can be distinguished.
Aguilera
R
R
Service Central d'Analyse, CNRS, Vernaison, France.
Becchi
M
M
Mateus
L
L
Popot
M A
MA
Bonnaire
Y
Y
Casabianca
H
H
Hatton
C K
CK
D000818:Animals; D000893:Anti-Inflammatory Agents / Q000737:chemistry / Q000652:urine*; D002247:Carbon Isotopes; D004300:Doping in Sports; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods*; D006736:Horses / Q000378:metabolism / Q000652:urine*; D006854:Hydrocortisone / Q000737:chemistry / Q000652:urine*
D000893:Anti-Inflammatory Agents; D002247:Carbon Isotopes; D006854:Hydrocortisone