title: Alteration of interendothelial adherens junctions following tumor cell-endothelial cell interaction in vitro.
abstract: The integrity of the vascular endothelium is mainly dependent upon the organization of interendothelial adherens junctions (AJ). These junctions are formed by the homotypic interaction of a transmembrane protein, vascular endothelial cadherin (VE-cadherin), which is complexed to an intracellular protein network including alpha-, beta-, and gamma-catenin. Additional proteins such as vinculin and alpha-actinin have been suggested to link the VE-cadherin/catenin complex to the actin-based cytoskeleton. During the process of hematogenous metastasis, circulating tumor cells must disrupt these intercellular junctions in order to extravasate. In the present study, we have investigated the influence of tumor cell-endothelial cell interaction upon interendothelial AJ. We show that human breast adenocarcinoma cells (MCF-7), but not normal human mammary epithelial cells, induce a rapid endothelial cell (EC) dissociation which correlates with the loss of VE-cadherin expression at the site of tumor cell-EC contact and with profound changes in vinculin distribution and organization. This process could not be inhibited by metalloproteinase nor serine protease inhibitors. Immunoprecipitations and Western blot analysis demonstrate that the overall expression of VE-cadherin and vinculin as well as the composition of the VE-cadherin/catenins complex are not affected by tumor cells while the tyrosine phosphorylation status of proteins within the complex is significantly altered. Our data suggest that tumor cells modulate AJ protein distribution and phosphorylation in EC and may, thereby, facilitate EC dissociation.
authors.affiliation: Laboratory of Cellular Biology, University of Liége, CHU, Sart-Tilman, Belgium. JM.Lewalle@ulg.ac.be
authors.forename: J M
authors.initials: JM
authors.lastname: Lewalle
authors.forename: K
authors.initials: K
authors.lastname: Bajou
authors.forename: J
authors.initials: J
authors.lastname: Desreux
authors.forename: M
authors.initials: M
authors.lastname: Mareel
authors.forename: E
authors.initials: E
authors.lastname: Dejana
authors.forename: A
authors.initials: A
authors.lastname: Noël
authors.forename: J M
authors.initials: JM
authors.lastname: Foidart
mesh_terms: D000230:Adenocarcinoma / Q000473:pathology*; D001943:Breast Neoplasms / Q000473:pathology*; D015820:Cadherins / Q000502:physiology*; D002448:Cell Adhesion; D003598:Cytoskeletal Proteins / Q000378:metabolism; D051180:Desmoplakins; D004730:Endothelium, Vascular / Q000648:ultrastructure*; D005260:Female; D006801:Humans; D007365:Intercellular Junctions / Q000648:ultrastructure*; D008855:Microscopy, Electron, Scanning; D010766:Phosphorylation; D019000:Phosphotyrosine / Q000378:metabolism; D011485:Protein Binding; D015534:Trans-Activators; D014407:Tumor Cells, Cultured; D016596:Vinculin / Q000378:metabolism; D051178:alpha Catenin; D051176:beta Catenin; D051185:gamma Catenin
chemical_list: C495273:CTNNA1 protein, human; C495270:CTNNB1 protein, human; D015820:Cadherins; D003598:Cytoskeletal Proteins; D051180:Desmoplakins; C495292:JUP protein, human; D015534:Trans-Activators; D051178:alpha Catenin; D051176:beta Catenin; D051185:gamma Catenin; D016596:Vinculin; D019000:Phosphotyrosine