title: Electron tomography of large, multicomponent biological structures.
abstract: Electron tomography is an extremely useful method for deriving three-dimensional structure from electron microscope images. The application of this technique to the reconstruction of large, complex structures such as mitochondria is described in conjunction with several tools for segmentation, measurement, classification, and visualization. In addition, the use of massively parallel computers to perform the tomographic reconstruction efficiently using R-weighted backprojection or iterative techniques is described.
authors.affiliation: Department of Biology, San Diego State University, California 92182-4614, USA.
authors.forename: G A
authors.initials: GA
authors.lastname: Perkins
authors.forename: C W
authors.initials: CW
authors.lastname: Renken
authors.forename: J Y
authors.initials: JY
authors.lastname: Song
authors.forename: T G
authors.initials: TG
authors.lastname: Frey
authors.forename: S J
authors.initials: SJ
authors.lastname: Young
authors.forename: S
authors.initials: S
authors.lastname: Lamont
authors.forename: M E
authors.initials: ME
authors.lastname: Martone
authors.forename: S
authors.initials: S
authors.lastname: Lindsey
authors.forename: M H
authors.initials: MH
authors.lastname: Ellisman
mesh_terms: D000465:Algorithms; D000818:Animals; D001369:Axons / Q000648:ultrastructure; D002540:Cerebral Cortex / Q000648:ultrastructure; D003198:Computer Simulation; D007091:Image Processing, Computer-Assisted; D008854:Microscopy, Electron / Q000379:methods*; D008928:Mitochondria / Q000648:ultrastructure; D008961:Models, Structural; D051381:Rats; D014054:Tomography / Q000379:methods