A self-assembled antibody electrode for HT29 tumour cells.
The present paper describes a self-assembled monoclonal-antibody immunosensor for HT29 tumour cells. This immunosensor is composed of two parts. One is a three-electrode system and the other is a layer of anti-(human colonic tumour) monoclonal antibody DF-I, which self-assembled on to the gold electrode. Two kinds of cells, HT29 tumour cells and normal fibroblast cells, were compared using the antibody electrode. The result was the same as that obtained by using immune luminescence. The HT29 cells can be captured specifically by the assembled anti-HT29 monoclonal-antibody electrode and can be measured by an electrochemical method.
Feng
J
J
Department of Chemistry, Peking University, Beijing, Peoples' Republic of China.
Zhu
L
L
Ci
Y X
YX
Wang
X Y
XY
Guo
Z Q
ZQ
D000911:Antibodies, Monoclonal; D015374:Biosensing Techniques; D001709:Biotechnology; D004563:Electrochemistry; D004566:Electrodes; D016650:Fluorescein-5-isothiocyanate; D005455:Fluorescent Antibody Technique; D019073:HT29 Cells; D006801:Humans; D013997:Time Factors
D000911:Antibodies, Monoclonal; D016650:Fluorescein-5-isothiocyanate