title: Characterization of a cDNA clone encoding pedal peptide in the terrestrial snail.
abstract: We report the isolation of a Helix lucorum cDNA clone encoding a precursor of neuropeptides that are closely related to Aplysia and Tritonia pedal peptides (Pep). The predicted propeptide contains 20 copies of the two variants of Helix Pep interspersed with Lys-Arg endopeptidase cleavage sites. Northern blot hybridization revealed multiple Pep-hybridizing species in the Helix CNS RNA. The Pep gene was expressed by several identified serotonergic neurones in pedal and cerebral ganglia, groups of sensory neurones in procerebrum, peripheral neurones in olfactory bulb, mantle and foot, and group of neurones in pedal ganglia presumably involved in locomotion control. Pep mRNA was detected in several neurones at the early stages of nervous system development.
authors.affiliation: Laboratory of Molecular Bases of Differentiation and Development, Engelhardt Institute of Molecular Biology, Moscow, Russia.
authors.forename: D A
authors.initials: DA
authors.lastname: Poteryaev
authors.forename: I S
authors.initials: IS
authors.lastname: Zakharov
authors.forename: P M
authors.initials: PM
authors.lastname: Balaban
authors.forename: P N
authors.initials: PN
authors.lastname: Uvarov
authors.forename: A V
authors.initials: AV
authors.lastname: Belyavsky
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001048:Aplysia / Q000378:metabolism; D003001:Cloning, Molecular; D018076:DNA, Complementary; D017952:Ganglia, Invertebrate / Q000378:metabolism*; D006372:Helix, Snails / Q000378:metabolism*; D008969:Molecular Sequence Data; D009474:Neurons / Q000378:metabolism; D009479:Neuropeptides / Q000096:biosynthesis* / Q000737:chemistry; D011498:Protein Precursors / Q000096:biosynthesis / Q000737:chemistry; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid
chemical_list: D018076:DNA, Complementary; D009479:Neuropeptides; D011498:Protein Precursors; C067889:pedal peptide