Evaluation of p53 mutation in pancreatic acinar cell carcinomas of humans and transgenic mice.
Mutation of the p53 tumor suppressor gene is found in a large number of exocrine pancreatic tumors. The majority of these tumors is of the ductal cell phenotype. We examined 12 human acinar cell carcinomas and 42 transgenic mouse carcinomas (including 36 acinar cell tumors, four islet cell tumors, and two liver metastases of primary acinar cell tumors) for evidence of p53 mutation. Immunohistochemistry was used to identify p53 protein in tumor sections. To evaluate p53 exons 5-8, heteroduplex analysis was used on formalin-fixed, paraffin-embedded human tumor DNA, and single-strand conformation polymorphism analysis was used on frozen mouse tumor DNA. No molecular evidence of p53 mutation was found in any of the tumor DNAs and immunohistochemical data were regarded as negative. This study provides evidence that acinar cell carcinogenesis in both humans and transgenic mice is independent of p53 mutation.
Terhune
P G
PG
Department of Pathology, Dartmouth Medical School, Lebanon, New Hampshire 03756, USA.
Memoli
V A
VA
Longnecker
D S
DS
D000818:Animals; D000911:Antibodies, Monoclonal; D018267:Carcinoma, Acinar Cell / Q000235:genetics*; D004273:DNA, Neoplasm / Q000032:analysis; D016158:Genes, p53; D006801:Humans; D007124:Immunoenzyme Techniques; D051379:Mice; D008822:Mice, Transgenic; D009154:Mutation; D009692:Nucleic Acid Heteroduplexes / Q000032:analysis; D010190:Pancreatic Neoplasms / Q000235:genetics*; D016133:Polymerase Chain Reaction; D018807:Polymorphism, Single-Stranded Conformational; D016159:Tumor Suppressor Protein p53 / Q000032:analysis
D000911:Antibodies, Monoclonal; D004273:DNA, Neoplasm; D009692:Nucleic Acid Heteroduplexes; D016159:Tumor Suppressor Protein p53