title: Nafazatrom inhibits peroxidation of phosphatidylcholine liposomes, heart homogenate and low density lipoproteins.
abstract: In order to contribute to the understanding of the biological properties of nafazatrom, an antithrombotic agent (NAP), we studied its effects on peroxidation of low density lipoproteins (LDL), lipid liposomes, heart homopgenate, and its interaction with alpha-tocopherol radical. NAP decreased the FeSO4 and H202-induced peroxidation of phosphatidylcholine liposomes and heart homogenate, and it decreased peroxidation of LDL induced by CuSO4 or 2,2'-azobis(2-amidinopropane). The antioxidant effect of NAF was about 3 times less potent than that of alpha-tocopherol (alpha-TOC) in phosphatidylcholine liposomes, and NAF was about 2-4 times more efficient to decrease peroxidation of LDL than alpha-TOC. Possible interaction of NAF with alpha-tocopherol radical (alpha-TR) was studied by EPR spectroscopy. NAF decreased the concentration of alpha-TR, but it was about 100-times less efficient than vitamin C. This may indicate that NAF does not interfere with alpha-TR formation and/or reduction of alpha-TR in biological system. The obtained results may help the explanation of biological effects of NAF.
authors.affiliation: Institute of Molecular Physiology and Genetics, Slovak Academy of Sciences, Bratislava, Slovakia. umfgonds@kramare.savba.sk
authors.forename: K
authors.initials: K
authors.lastname: Ondrias
authors.forename: A
authors.initials: A
authors.lastname: Stasko
authors.forename: D
authors.initials: D
authors.lastname: Gergèl
authors.forename: E
authors.initials: E
authors.lastname: Ondriasovà
authors.forename: M
authors.initials: M
authors.lastname: Hromadová
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000494:pharmacology*; D004578:Electron Spin Resonance Spectroscopy; D005343:Fibrinolytic Agents / Q000494:pharmacology*; D006321:Heart / Q000187:drug effects; D006801:Humans; D015227:Lipid Peroxidation / Q000187:drug effects*; D008077:Lipoproteins, LDL / Q000378:metabolism*; D008081:Liposomes; D009206:Myocardium / Q000378:metabolism*; D010713:Phosphatidylcholines / Q000378:metabolism*; D011720:Pyrazoles / Q000494:pharmacology*; D047069:Pyrazolones; D051381:Rats; D014810:Vitamin E / Q000494:pharmacology
chemical_list: D000975:Antioxidants; D005343:Fibrinolytic Agents; D008077:Lipoproteins, LDL; D008081:Liposomes; D010713:Phosphatidylcholines; D011720:Pyrazoles; D047069:Pyrazolones; D014810:Vitamin E; C018845:nafazatrom