title: In vitro antioxidant and cytotoxic activity of extracts of Baccharis coridifolia DC.
abstract: The in vitro antioxidant and cytotoxic activities in different extracts of Baccharis coridifolia DC. (Compositae) were studied using different bioassays. Antioxidant activity was tested using hydroperoxide-initiated chemiluminescence (CL) in rat liver homogenates. The aqueous extract showed antioxidant activity (IC50 = 141 microg/ml), while the dichloromethane and methanol extracts were prooxidant. Activity in the aqueous extract was confirmed by determination of thiobarbituric acid-reactive substances (TBARS), showing an IC50 = 556 microg/ml. On the other hand, studies on the cytotoxic activity demonstrated a 78% crown gall tumour inhibition and an ED50 = 4. 2 microg/ml in KB cells growth for the dichloromethane extract. A 56% decrease in the initial absorbance of DNA-Methyl Green complex could also be observed at 1000 microg/ml, suggesting the presence of compounds that interact with DNA.
authors.affiliation: Cátedra de Biotecnología y Microbiología Industrial, Facultad de Farmacia y Bioquímica, Universidad de Buenos Aires, Argentina.
authors.forename: E
authors.initials: E
authors.lastname: Mongelli
authors.forename: C
authors.initials: C
authors.lastname: Desmarchelier
authors.forename: J
authors.initials: J
authors.lastname: Rodríguez Talou
authors.forename: J
authors.initials: J
authors.lastname: Coussio
authors.forename: G
authors.initials: G
authors.lastname: Ciccia
mesh_terms: D000818:Animals; D000972:Antineoplastic Agents, Phytogenic / Q000494:pharmacology*; D000975:Antioxidants / Q000494:pharmacology*; D004249:DNA Damage; D004354:Drug Screening Assays, Antitumor; D006801:Humans; D006861:Hydrogen Peroxide / Q000378:metabolism; D066298:In Vitro Techniques; D007624:KB Cells; D008099:Liver / Q000187:drug effects / Q000378:metabolism; D008163:Luminescent Measurements; D008297:Male; D016877:Oxidants / Q000378:metabolism; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology; D010946:Plants, Medicinal / Q000737:chemistry*; D051381:Rats; D017208:Rats, Wistar; D013020:South America; D017392:Thiobarbituric Acid Reactive Substances / Q000378:metabolism
chemical_list: D000972:Antineoplastic Agents, Phytogenic; D000975:Antioxidants; D016877:Oxidants; D010936:Plant Extracts; D017392:Thiobarbituric Acid Reactive Substances; D006861:Hydrogen Peroxide