Evolution of phosphagen kinase. VI. Isolation, characterization and cDNA-derived amino acid sequence of lombricine kinase from the earthworm Eisenia foetida, and identification of a possible candidate for the guanidine substrate recognition site.
Lombricine kinase (LK) from the body wall muscle of the earthworm Eisenia foetida was purified to homogeneity. The enzyme was shown to be a dimer consisting of 40 kDa subunits. The cDNA-derived amino acid sequence of 370 residues of Eisenia LK was determined. The validity of the sequence was supported by chemical sequencing of internal tryptic peptides. This is the first reported lombricine kinase amino acid sequence. Alignment of Eisenia LK with those of creatine kinases (CKs), arginine kinases (AKs) and glycocyamine kinase (GK) suggested a region displaying remarkable amino acid deletions (referred to GS region), as a possible candidate for guanidine substrate recognition site. A phylogenetic analysis using amino acid sequences of all four phosphagen kinases indicates that CK, GK and LK probably evolved from a common immediate ancestor protein.
Suzuki
T
T
Department of Biology, Faculty of Science, Kochi University, Japan. suzuki@cc.kochi-u.ac.jp
Kawasaki
Y
Y
Furukohri
T
T
Ellington
W R
WR
D000595:Amino Acid Sequence; D000818:Animals; D001122:Arginine Kinase / Q000737:chemistry; D001483:Base Sequence; D001665:Binding Sites; D003402:Creatine Kinase / Q000737:chemistry; D019143:Evolution, Molecular; D006146:Guanidines / Q000378:metabolism; D008969:Molecular Sequence Data; D009835:Oligochaeta / Q000145:classification / Q000201:enzymology* / Q000235:genetics; D010455:Peptides / Q000032:analysis; D017852:Phosphotransferases (Nitrogenous Group Acceptor) / Q000737:chemistry* / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D011485:Protein Binding; D016415:Sequence Alignment; D017421:Sequence Analysis; D017384:Sequence Deletion / Q000235:genetics; D014357:Trypsin / Q000378:metabolism
D006146:Guanidines; D010455:Peptides; D017852:Phosphotransferases (Nitrogenous Group Acceptor); C023839:glycocyamine phosphokinase; D003402:Creatine Kinase; D001122:Arginine Kinase; C021517:lombricine kinase; D014357:Trypsin