[Effect of tobacco combustion on the immunochemical properties of albumin].
During tobacco burning smoker to run up substances to contain smoke as far as pulmonary tissue that is damage. In cigarette 600 degrees C are in ignition extreme, but in the other side, in contact with edge of the mouth smoker, the temperature is lower. Smoke could be delivery tobacco products until respiratory tract when temperature gradients occur in cigarette burn. For demonstration of the immunoreactive substances in tobacco smoke condense (TSC) we used a model with two cigarette arrangements: several concentrations of bovine seric albumin (BSA) applied to experimental group of cigarettes and phosphate-saline solution (PBS), 0.15 M pH 7.5 without protein to control cigarettes. Both series, experimental and control, remained at 20 degrees C during 48 h, soon afterward TSC was obtained. Higher protein concentration was observe in the experimental TSC of cigarettes expose to more elevated quantities of BSA, this was identify with polyclonal antibodies toward BSA employing counter-immunoelectrophoresis, immunoelectrophoresis, radial immunodiffusion and hemagglutination inhibition test. In summary: TSC of treat cigarettes had a little quantity of protein (BSA), but immunochemical properties of BSA in TSC were preserve because polyclonal antibodies against BSA bind to this protein. In habitual smoker some compounds present in cigarette smoke could be induce an immune response due to immunogen in tobacco substances.
Martínez
R D
RD
Departamento de medicina experimental, Facultad de Medicina, Universidad Nacional Autónoma de México, DF, México.
Chávez
R
R
D000818:Animals; D000906:Antibodies / Q000276:immunology; D002417:Cattle; D006358:Hot Temperature; D006801:Humans; D007158:Immunologic Techniques; D010940:Plant Proteins / Q000032:analysis / Q000276:immunology; D010947:Plants, Toxic; D011817:Rabbits; D012130:Respiratory Hypersensitivity / Q000209:etiology; D012710:Serum Albumin, Bovine / Q000032:analysis / Q000737:chemistry / Q000276:immunology*; D012906:Smoke / Q000032:analysis*; D014026:Nicotiana / Q000737:chemistry*
D000906:Antibodies; D010940:Plant Proteins; D012906:Smoke; D012710:Serum Albumin, Bovine