title: Allergic rhinitis to grass pollen: measurement of inflammatory mediators of mast cell and eosinophils in native nasal fluid lavage and in serum out of and during pollen season.
abstract: BACKGROUND
  In allergic rhinitis, mast cells, activated by cross-linking of allergen to mast cell-bound specific IgE, release both vasoactive mediators related to the early nasal symptoms and chemotactic mediators that attract inflammatory cells, such as eosinophils, related to the late-phase response.
  
  
  OBJECTIVE
  We have analyzed, during and out of pollen season, in blood and nasal fluid from patients allergic to grass pollen, histamine and tryptase to monitor the early phase markers and eosinophil and eosinophil cationic protein (ECP) to monitor the late phase.
  
  
  METHODS
  Twenty patients were enrolled in the study. As a control, we studied 10 nonatopic subjects. Mediators and eosinophils were assessed in blood and nasal fluid. Histamine was tested only in nasal fluid.
  
  
  RESULTS
  During pollen season, tryptase but not histamine increased in nasal fluids from patients (2.96 vs 0.22 U/ml, p = 0.001) and correlated with symptom scores (r(s) = 0.63, p = 0.003). Tryptase was not detected in serum. Eosinophils increased in nasal cytology (17.0% vs 2.0%, p = 0.001) and in the blood (265 vs 12.7 x 10(6) L, p = 0.001) from patients, but they did not correlate with symptom scores. ECP increased only in the nasal lavage (1633 vs 1.30 ng/ml, p = 0.001) and correlated with symptom scores (r(s) = 0.53, p = 0.016).
  
  
  CONCLUSIONS
  Both ECP and tryptase increase in nasal secretion in natural disease. Therefore the measurement of tryptase and ECP levels in nasal fluid might be a useful clinical test for monitoring disease activity and the effects of therapeutic agents.
authors.affiliation: Istituto di Medicina Interna e Geriatria, Università degli Studi di Palermo, Italy.
authors.forename: G
authors.initials: G
authors.lastname: Di Lorenzo
authors.forename: P
authors.initials: P
authors.lastname: Mansueto
authors.forename: M
authors.initials: M
authors.lastname: Melluso
authors.forename: G
authors.initials: G
authors.lastname: Candore
authors.forename: A
authors.initials: A
authors.lastname: Colombo
authors.forename: M E
authors.initials: ME
authors.lastname: Pellitteri
authors.forename: A
authors.initials: A
authors.lastname: Drago
authors.forename: M
authors.initials: M
authors.lastname: Potestio
authors.forename: C
authors.initials: C
authors.lastname: Caruso
mesh_terms: D000328:Adult; D004804:Eosinophils / Q000737:chemistry* / Q000187:drug effects; D005260:Female; D006801:Humans; D006969:Hypersensitivity, Immediate / Q000097:blood / Q000276:immunology / Q000378:metabolism; D018836:Inflammation Mediators / Q000032:analysis* / Q000097:blood* / Q000494:pharmacology; D007958:Leukocyte Count; D008297:Male; D008407:Mast Cells / Q000737:chemistry* / Q000187:drug effects; D017817:Nasal Lavage Fluid; D006109:Poaceae / Q000276:immunology; D011058:Pollen / Q000276:immunology*; D006255:Rhinitis, Allergic, Seasonal / Q000097:blood / Q000276:immunology* / Q000378:metabolism; D012621:Seasons
chemical_list: D018836:Inflammation Mediators