Cloning and characterization of the ori region of pSW1200 of Erwinia stewartii: similarity with plasmid P1.
The ori region of an Erwinia stewartii plasmid, pSW1200 (106 kb), has been cloned and sequenced. This region consists of a gene encoding a protein which has 91% similarity and 73% identity with the RepA protein of bacteriophage P1. The ori region also consists of eight copies of 19-bp iterons which are highly homologous to the iterons of P1. Similar to plasmid P1, pSW1200 replicon has a copy number of approximately 1. On the other hand, the copy number increases about ninefold if three of the iterons located downstream from repA gene are deleted. We also demonstrate that pGEM-5Z consisting of a copy of P1 iteron is incompatible with a pSW1200 derivative, pSW1201, suggesting that pSW1200 and P1 DNA are incompatible and both belong to the IncY group.
Fu
J F
JF
Department of Microbiology and Immunology, Chang-Gung University, Taoyuan, Taiwan.
Ying
S W
SW
Liu
S T
ST
D001483:Base Sequence; D015139:Blotting, Southern; D003001:Cloning, Molecular; D004252:DNA Mutational Analysis; D004269:DNA, Bacterial; D004885:Erwinia / Q000235:genetics*; D018628:Gene Dosage; D008969:Molecular Sequence Data; D010957:Plasmids; D018741:Replication Origin; D012093:Replicon; D017422:Sequence Analysis, DNA
D004269:DNA, Bacterial