title: A floral third whorl-specific marker gene in the dioecious species white campion is differentially expressed in mutants defective in stamen development.
abstract: The CCLS4 gene of white campion is specifically expressed in anther epidermis and endothecium from pre- to post-meiotic stages. We report on a detailed in situ analysis of the gene's expression and show that it is a marker of the floral third whorl. The gene is expressed (1) in the anther (epidermis, parietal cells and the derived endothecium) in normally developing stamens and (2) in distinct sub-domains of third-whorl epidermis in mutants exhibiting aberrant states of parietal differentiation. Our results suggest that CCLS4 may fulfil different functions during pre- and post-meiotic anther development and reveal the complex role parietal cells may play during early stamen formation.
authors.affiliation: Vrije Universiteit Brussel, Belgium.
authors.forename: S
authors.initials: S
authors.lastname: Hinnisdaels
authors.forename: A
authors.initials: A
authors.lastname: Lardon
authors.forename: N
authors.initials: N
authors.lastname: Barbacar
authors.forename: I
authors.initials: I
authors.lastname: Negrutiu
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D015152:Blotting, Northern; D002454:Cell Differentiation / Q000235:genetics; D018506:Gene Expression Regulation, Plant; D005819:Genetic Markers; D008969:Molecular Sequence Data; D009154:Mutation; D009928:Organ Specificity / Q000235:genetics; D059828:Plant Cells; D063245:Plant Development; D010940:Plant Proteins / Q000096:biosynthesis / Q000235:genetics*; D010944:Plants / Q000235:genetics*; D017422:Sequence Analysis, DNA; D012727:Sex Characteristics; D014158:Transcription, Genetic
chemical_list: C109819:CCLS4 protein, white campion; D005819:Genetic Markers; D010940:Plant Proteins