Quantification by additive RT-PCR of HIV-1 RNA plasma levels in different stages of HIV-1 infection.
In this study, virion-associated RNA was measured in plasma from twenty six patients in various stages of HIV-1 disease by the additive RT-PCR method. Plasma viral RNA levels were inversely correlated (r = -0.72894) with total CD4+ cell counts and directly (r = 0.86964) with serum titre beta 2-microglobulin in chronically infected patients. This additive RT-PCR is based on a mathematical logistic adjustment of the standard curve and the use of an internal standard identical to the target molecule, which represents a control system for the efficiency of RT-PCR and allows a continuous assessment of the accuracy based on the recovery.
Fernández-Arcás
N
N
Departamento de Bioquímica y Biología Molecular, Facultad de Medicina, Universidad de Málaga, Spain.
Diéguez-Lucena
J L
JL
Costa
A F
AF
García-Villanova
J
J
Ruiz-Galdón
M
M
Peña
J
J
Morell
M
M
Reyes-Engel
A
A
D000163:Acquired Immunodeficiency Syndrome / Q000821:virology*; D016022:Case-Control Studies; D015497:HIV-1 / Q000235:genetics / Q000302:isolation & purification*; D006801:Humans; D016015:Logistic Models; D016133:Polymerase Chain Reaction / Q000379:methods*; D012367:RNA, Viral / Q000302:isolation & purification; D015203:Reproducibility of Results; D014158:Transcription, Genetic
D012367:RNA, Viral