Periplasmic secretion of functional ovotransferrin N-lobe in Escherichia coli.
The cytoplasmic and periplasmic production systems of ovotransferrin N-lobe in Escherichia coli were constructed. The periplasmic, but not cytoplasmic product, was found to have the iron-binding function.
Miyauchi
T
T
Research Institute for Food Science, Kyoto University, Japan.
Takahashi
N
N
Hirose
M
M
D001425:Bacterial Outer Membrane Proteins / Q000235:genetics / Q000378:metabolism; D002352:Carrier Proteins / Q000378:metabolism; D003207:Conalbumin / Q000235:genetics / Q000378:metabolism*; D003593:Cytoplasm / Q000378:metabolism; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism*; D033862:Iron-Binding Proteins; D009154:Mutation; D019897:Periplasm / Q000378:metabolism*; D033863:Transferrin-Binding Proteins
D001425:Bacterial Outer Membrane Proteins; D002352:Carrier Proteins; D033862:Iron-Binding Proteins; D033863:Transferrin-Binding Proteins; D003207:Conalbumin