Paternity assessment in rhesus macaques (Macaca mulatta): multilocus DNA fingerprinting and PCR marker typing.
Establishing kinship relations in primates using modern molecular genetic techniques has enhanced the ability to scrutinize a number of fundamental biological issues. We screened 51 human short tandem repeats (STRs) for cross-species PCR amplification in rhesus macaques (Macaca mulatta) and identified 11 polymorphic loci with heterozygosity rates of at least 0.6. These markers were used for paternity testing in three social groups (M, R, and S) of rhesus macaques from Cayo Santiago, Puerto Rico. Several consecutive birth cohorts were analyzed in which approximately 200 males were tested for paternity against more than 100 mother/ infant pairs. Despite a combined exclusion rate of more than 99.9% in all three groups, some cases could not be solved unequivocally with the STR markers and additional testing of the MHC-associated DQB1 polymorphism. A final decision became possible through multilocus DNA fingerprinting with one or more of the oligonucleotide probes (GATA)4, (CA)8, and (CAC)5. Paternity assessment by multilocus DNA analysis with probe (CAC)5 alone was found to have limitations in rhesus macaques as regards the number of potential sires which might be involved in a given case. Multilocus DNA fingerprinting requires large amounts of DNA, and the ensuing autoradiographic patterns present difficulties in comparisons across gels and even within the same gel across remote lanes. Computer-assisted image analysis was incapable of eliminating this problem. Therefore, a dual approach to DNA typing has been adopted, using STR markers to reduce the number of potential sires to a level where all remaining candidates can be tested by multilocus DNA fingerprinting on a single gel, preferably in lanes adjacent to the mother/infant pair.
Nürnberg
P
P
Institut für Medizinische Genetik, Universitätsklinikum Charité, Berlin, Germany.
Sauermann
U
U
Kayser
M
M
Lanfer
C
C
Manz
E
E
Widdig
A
A
Berard
J
J
Bercovitch
F B
FB
Kessler
M
M
Schmidtke
J
J
Krawczak
M
M
D000818:Animals; D015331:Cohort Studies; D004247:DNA / Q000097:blood; D016172:DNA Fingerprinting / Q000191:economics / Q000379:methods / Q000662:veterinary*; D015343:DNA Probes, HLA / Q000235:genetics; D005260:Female; D005819:Genetic Markers; D006801:Humans; D008253:Macaca mulatta / Q000097:blood / Q000235:genetics*; D008297:Male; D018895:Microsatellite Repeats; D010334:Paternity; D016133:Polymerase Chain Reaction / Q000662:veterinary
D015343:DNA Probes, HLA; D005819:Genetic Markers; D004247:DNA