Improved gas chromatographic-mass spectrometric method for the quantitative determination of vinpocetine in human plasma.
An improved and validated method for the determination of vinpocetine in human plasma using a combination of a solid-phase extraction as a cleaning step followed by gas chromatography-mass spectrometry (GC-MS) has been presented. Quantitation has been carried out with apovincaminic acid methyl ester as internal standard. A limit of detection of 0.01 ng/ml, limit of quantitation of 0.10 ng/ml, as well as a satisfactory accuracy, improved precision and prolonged capillary column life have been achieved.
Vatsova
M
M
Chemical Pharmaceutical Research Institute, Sofia, Bulgaria.
Tzvetanov
S
S
Drenska
A
A
Goranscheva
J
J
Tyutyulkova
N
N
D000959:Antihypertensive Agents / Q000097:blood* / Q000737:chemistry; D002940:Circadian Rhythm; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods*; D006801:Humans; D016014:Linear Models; D009994:Osmolar Concentration; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D014748:Vinca Alkaloids / Q000097:blood* / Q000737:chemistry
D000959:Antihypertensive Agents; D014748:Vinca Alkaloids; C013983:vinpocetine