Partial purification and characterization of a gibberellin-binding protein from seedlings of Azukia angularis.
Little is known about mechanisms for the perception of gibberellin (GA), a phytohormone, by plants. A GA-binding protein (GBP) with receptor-like characteristics was partially purified from azuki bean (Azukia angularis) seedlings. Gibberellins that caused much elongation of azuki epicotyl sections showed strong binding affinity for the GBP, and their biosynthetic precursors and inactive metabolites had weak affinity for it. The apparent Kd value for GA4 was calculated to be 7 x 10(-10) M from Scatchard plots. The molecular weight of the GBP in the native state was estimated to be 25,000 by high-performance liquid chromatography with a gel-permeation column. The GBP may be one of the receptor candidates for GAs.
Nakajima
M
M
Department of Applied Biological Chemistry, University of Tokyo, Japan. anakajm@hongo.ecc.u-tokyo.ac.jp
Takita
K
K
Wada
H
H
Mihara
K
K
Hasegawa
M
M
Yamaguchi
I
I
Murofushi
N
N
D002352:Carrier Proteins / Q000737:chemistry* / Q000302:isolation & purification*; D002850:Chromatography, Gel; D007887:Fabaceae; D005875:Gibberellins / Q000378:metabolism* / Q000494:pharmacology; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D008970:Molecular Weight; D010750:Phosphoproteins / Q000737:chemistry* / Q000302:isolation & purification*; D010940:Plant Proteins / Q000737:chemistry* / Q000302:isolation & purification*; D010946:Plants, Medicinal; D011485:Protein Binding; D012639:Seeds / Q000737:chemistry
D002352:Carrier Proteins; D005875:Gibberellins; D010750:Phosphoproteins; D010940:Plant Proteins; C099131:gibberellin-binding phosphoprotein, plant