Characterization for virulence of an axenic strain of Entamoeba histolytica: evidence for overexpression of two proteins.
The pattern of protein from membrane and crude homogenate of Entamoeba histolytica strain 462 axenically cultivated (462ac) and submitted to hamster liver passage (462hp) was obtained by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. The substrains 462ac and 462hp were compared by zymodeme analysis, erythrophagocytosis, cytopathic effect upon mammalian cells and the capability to induce abscess in hamster liver. The results showed no differences for erythrophagocytosis, cytopathic effect or zymodene for substrains 462ac and 462hp. A type II pathogenic zymodene was observed. Substrain 462ac did not induce liver abscess, but 462hp induced abscesses in 70% of the inoculated animals. The pattern of proteins from plasma membrane and crude homogenate were different. One protein of approximately 45kDa and another of 23 kDa showed at no detectable levels in the membrane of 462ac. A third component of approximately 90 kDa showed more intensively expressed in the 462ac.
Gomes
M A
MA
Department of Parasitology, Institute of Biological Services, Federal University of Minas Gerais, Belo Horizonte, Brazil.
Melo
M N
MN
Pesquero
J L
JL
Silva
E F
EF
D000818:Animals; D002522:Chlorocebus aethiops; D006224:Cricetinae; D004591:Electrophoresis, Polyacrylamide Gel; D004748:Entamoeba histolytica / Q000378:metabolism* / Q000472:pathogenicity*; D004749:Entamoebiasis / Q000378:metabolism / Q000469:parasitology; D005260:Female; D006801:Humans; D008100:Liver Abscess / Q000378:metabolism / Q000469:parasitology; D008297:Male; D015800:Protozoan Proteins / Q000096:biosynthesis*; D012967:Sodium Dodecyl Sulfate; D014709:Vero Cells; D014774:Virulence
D015800:Protozoan Proteins; D012967:Sodium Dodecyl Sulfate