Analysis of biofluids and chemical mixtures in non-deuterated solvents with 1H diffusion-weighted PFG phase-sensitive double-quantum NMR spectroscopy.
Two different types of one- and two-dimensional diffusion-weighted H phase-sensitive pulsed field gradient double-quantum experiments (DWDQ) are presented and their application to a biological plasma sample is shown. The remarkable solvent suppression achieved with these pulse sequences and the high sensitivity of these experiments allows the determination of the diffusion coefficients of molecules at low concentration dissolved in non-deuterated solvents. Biofluids and complex chemical mixtures from combinatorial chemistry and from natural sources can be analysed with these experiments, and the different molecular species of the mixtures can be identified and assigned, based on their different diffusion coefficients.
Dalvit
C
C
NOVARTIS AG, CH-4002 Basel, Switzerland.
Böhlen
J M
JM
D000596:Amino Acids / Q000097:blood; D000818:Animals; D001774:Blood Chemical Analysis / Q000379:methods*; D001786:Blood Glucose / Q000032:analysis; D019343:Citric Acid / Q000097:blood; D003903:Deuterium; D004058:Diffusion; D019344:Lactic Acid / Q000097:blood; D008077:Lipoproteins, LDL / Q000097:blood; D008079:Lipoproteins, VLDL / Q000097:blood; D008297:Male; D019906:Nuclear Magnetic Resonance, Biomolecular / Q000379:methods*; D011522:Protons; D011789:Quantum Theory; D051381:Rats; D017207:Rats, Sprague-Dawley; D012680:Sensitivity and Specificity; D012997:Solvents
D000596:Amino Acids; D001786:Blood Glucose; D008077:Lipoproteins, LDL; D008079:Lipoproteins, VLDL; D011522:Protons; D012997:Solvents; D019343:Citric Acid; D019344:Lactic Acid; D003903:Deuterium