Bone-related matrix proteins expression in vitro and in vivo by marrow stromal cell line.
MBA-1, a bone marrow stroma-derived cell line, was transplanted in an ectopic site and formed endochondral bone. The ossicle developed through stages of cell proliferation, differentiated into a zone of hypertrophy and formed a chondroid-like area which further developed to primary mineralized bone. We explored the expression of various matrix proteins by MBA-1 cells in vitro and in the ossicle formed in vivo. MBA-1 cells constitutively expressed mRNAs encoding for collagen I, non-collagenous proteins and alkaline phosphatase. RNA extracted from the ossicle formed by these cells was expressed in a different pattern. The in vivo maturation of MBA-1 cells was accompanied by low expression of mRNA for procollagen alpha 2(I) and a marked increase in osteonectin and osteopontin mRNA levels. Thus, the ability to follow expression of these genes through bone formation in vivo has been demonstrated.
Benayahu
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Department of Histology and Cell Biology, Sackler Faculty of Medicine, Tel Aviv University, Israel.
Gurevitz
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OA
Shamay
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D000199:Actins / Q000032:analysis; D000469:Alkaline Phosphatase / Q000032:analysis; D000818:Animals; D058578:Biglycan; D001846:Bone Development / Q000502:physiology*; D001854:Bone Marrow Cells / Q000737:chemistry / Q000166:cytology* / Q000201:enzymology; D001857:Bone Matrix / Q000737:chemistry* / Q000166:cytology / Q000502:physiology; D002448:Cell Adhesion; D002460:Cell Line; D003094:Collagen / Q000235:genetics; D016326:Extracellular Matrix Proteins; D015870:Gene Expression / Q000502:physiology; D007668:Kidney / Q000166:cytology; D051379:Mice; D008819:Mice, Nude; D015676:Osteonectin / Q000235:genetics; D053495:Osteopontin; D011509:Proteoglycans / Q000235:genetics; D012333:RNA, Messenger / Q000032:analysis; D012795:Sialoglycoproteins / Q000235:genetics; D017154:Stromal Cells / Q000737:chemistry / Q000166:cytology / Q000201:enzymology
D000199:Actins; C546885:Bgn protein, mouse; D058578:Biglycan; D016326:Extracellular Matrix Proteins; D015676:Osteonectin; D011509:Proteoglycans; D012333:RNA, Messenger; D012795:Sialoglycoproteins; C506896:Spp1 protein, mouse; D053495:Osteopontin; D003094:Collagen; D000469:Alkaline Phosphatase