title: Genetic variation of envelope gp90 gene of equine infectious anemia virus isolated from an experimentally infected horse.
abstract: Six strains of equine infectious anemia virus (EIAV) were recovered from febrile and non-febrile stages of a horse experimentally infected with the P337-V70 strain given once to a horse. The env gp90 genes of the isolates, the P337-V70 and P337-V26, avirulent virus derived from the P337-V70 strain, were sequenced. A comparison of the gp90 gene sequences revealed that amino acid variations among the viruses tested showed as high as 8.2 to 11.5%. In addition, the comparison also indicated that the isolates that recovered from the non-febrile stage were contained in nucleotide insertions in the principal neutralizing domain (PND) region. The insertions were arranged regularly with smaller segments. The nucleotide sequence of the P337-V26 gp90 gene was found to contain a six-nucleotides insertion and seven nucleotide substitutions outside the PND region, when compared with that of the P337-V70 strain.
authors.affiliation: National Institute of Animal Health, Ibaraki, Japan.
authors.forename: H
authors.initials: H
authors.lastname: Pang
authors.forename: X G
authors.initials: XG
authors.lastname: Kong
authors.forename: H
authors.initials: H
authors.lastname: Sentsui
authors.forename: Y
authors.initials: Y
authors.lastname: Kono
authors.forename: T
authors.initials: T
authors.lastname: Sugiura
authors.forename: A
authors.initials: A
authors.lastname: Hasegawa
authors.forename: H
authors.initials: H
authors.lastname: Akashi
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D004279:DNA, Viral / Q000032:analysis / Q000737:chemistry / Q000235:genetics*; D004859:Equine Infectious Anemia / Q000235:genetics*; D005260:Female; D005814:Genes, Viral / Q000235:genetics*; D014644:Genetic Variation; D006736:Horses; D004860:Infectious Anemia Virus, Equine / Q000235:genetics*; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction / Q000379:methods / Q000662:veterinary; D014759:Viral Envelope Proteins / Q000032:analysis / Q000737:chemistry / Q000235:genetics*
chemical_list: D004279:DNA, Viral; D014759:Viral Envelope Proteins