title: CLIP-115, a novel brain-specific cytoplasmic linker protein, mediates the localization of dendritic lamellar bodies.
abstract: Intracellular localization of organelles may depend in part on specific cytoplasmic linker proteins (CLIPs) that link membranous organelles to microtubules. Here, we characterize rat cDNAs encoding a novel, brain-specific CLIP of 115 kDa. This protein contains two N-terminal microtubule-binding domains and a long coiled-coil region; it binds to microtubules and is homologous to CLIP-170, a protein mediating the binding of endosomes to microtubules. CLIP-115 is enriched in the dendritic lamellar body (DLB), a recently discovered organelle predominantly present in bulbous dendritic appendages of neurons linked by dendrodendritic gap junctions. Local microtubule depolymerization leads to a temporary reduction of DLBs. These results suggest that CLIP-115 operates in the control of brain-specific organelle translocations.
authors.affiliation: Department of Anatomy, Erasmus University Rotterdam, The Netherlands.
authors.forename: C I
authors.initials: CI
authors.lastname: De Zeeuw
authors.forename: C C
authors.initials: CC
authors.lastname: Hoogenraad
authors.forename: E
authors.initials: E
authors.lastname: Goedknegt
authors.forename: E
authors.initials: E
authors.lastname: Hertzberg
authors.forename: A
authors.initials: A
authors.lastname: Neubauer
authors.forename: F
authors.initials: F
authors.lastname: Grosveld
authors.forename: N
authors.initials: N
authors.lastname: Galjart
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D001921:Brain / Q000166:cytology / Q000378:metabolism*; D019556:COS Cells; D003001:Cloning, Molecular; D003712:Dendrites / Q000378:metabolism* / Q000648:ultrastructure; D006801:Humans; D008297:Male; D008869:Microtubule-Associated Proteins / Q000032:analysis / Q000096:biosynthesis* / Q000737:chemistry; D008870:Microtubules / Q000187:drug effects / Q000378:metabolism / Q000648:ultrastructure; D008969:Molecular Sequence Data; D009363:Neoplasm Proteins; D009419:Nerve Tissue Proteins / Q000032:analysis / Q000096:biosynthesis* / Q000737:chemistry; D009474:Neurons / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D015739:Nocodazole / Q000494:pharmacology; D009847:Olivary Nucleus / Q000378:metabolism; D017239:Paclitaxel / Q000494:pharmacology; D051381:Rats; D017208:Rats, Wistar; D011994:Recombinant Proteins / Q000032:analysis / Q000096:biosynthesis; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid; D014162:Transfection
chemical_list: D008869:Microtubule-Associated Proteins; D009363:Neoplasm Proteins; D009419:Nerve Tissue Proteins; D011994:Recombinant Proteins; C109878:cytoplasmic linker protein 115; C076744:cytoplasmic linker protein 170; D017239:Paclitaxel; D015739:Nocodazole