title: In silicio identification of glycosyl-phosphatidylinositol-anchored plasma-membrane and cell wall proteins of Saccharomyces cerevisiae.
abstract: Use of the Von Heijne algorithm allowed the identification of 686 open reading frames (ORFs) in the genome of Saccharomyces cerevisiae that encode proteins with a potential N-terminal signal sequence for entering the secretory pathway. On further analysis, 51 of these proteins contain a potential glycosyl-phosphatidylinositol (GPI)-attachment signal. Seven additional ORFs were found to belong to this group. Upon examination of the possible GPI-attachment sites, it was found that in yeast the most probable amino acids for GPI-attachment as asparagine and glycine. In yeast, GPI-proteins are found at the cell surface, either attached to the plasma-membrane or as an intrinsic part of the cell wall. It was noted that plasma-membrane GPI-proteins possess a dibasic residue motif just before their predicted GPI-attachment site. Based on this, and on homologies between proteins, families of plasma-membrane and cell wall proteins were assigned, revealing 20 potential plasma-membrane and 38 potential cell wall proteins. For members of three plasma-membrane protein families, a function has been described. On the other hand, most of the cell wall proteins seem to be structural components of the wall, responsive to different growth conditions. The GPI-attachment site of yeast slightly differs from mammalian cells. This might be of use in the development of anti-fungal drugs.
authors.affiliation: Fungal Cell Wall Group Amsterdam, BioCentrum Amsterdam, University of Amsterdam, The Netherlands.
authors.forename: L H
authors.initials: LH
authors.lastname: Caro
authors.forename: H
authors.initials: H
authors.lastname: Tettelin
authors.forename: J H
authors.initials: JH
authors.lastname: Vossen
authors.forename: A F
authors.initials: AF
authors.lastname: Ram
authors.forename: H
authors.initials: H
authors.lastname: van den Ende
authors.forename: F M
authors.initials: FM
authors.lastname: Klis
mesh_terms: D000595:Amino Acid Sequence; D002462:Cell Membrane; D002473:Cell Wall; D005656:Fungal Proteins / Q000145:classification / Q000235:genetics*; D016681:Genome, Fungal; D017261:Glycosylphosphatidylinositols; D008565:Membrane Proteins / Q000235:genetics; D008969:Molecular Sequence Data; D010802:Phylogeny; D011499:Protein Processing, Post-Translational / Q000235:genetics; D021382:Protein Sorting Signals / Q000235:genetics; D012441:Saccharomyces cerevisiae / Q000235:genetics*; D017421:Sequence Analysis
chemical_list: D005656:Fungal Proteins; D017261:Glycosylphosphatidylinositols; D008565:Membrane Proteins; D021382:Protein Sorting Signals