title: Prognostic significance of DNA replication errors in young patients with colorectal cancer.
abstract: OBJECTIVE
  To determine the DNA replication error (RER) status in young patients with colorectal cancer (CRC), and to compare the clinical and pathologic characteristics of RER-positive and RER-negative cases.
  
  
  BACKGROUND
  Recent studies suggest that patients with RER-positive CRC have an improved prognosis. Further data are required to confirm this observation in young CRC patients.
  
  
  METHODS
  All patients 40 years of age and younger with CRC admitted to the National Naval Medical Center between 1970 and 1992 were considered for inclusion in the study. After review, 36 patients for whom the original archived pathology specimen could be retrieved served as the study population. The RER status was determined using a previously described polymerase chain reaction-based assay. The clinical and pathologic features and survival data were compared to RER status.
  
  
  RESULTS
  RER-positive tumors were found in 17 cases (47%). There was no significant difference in Dukes' stage or histologic grade at the time of diagnosis between patients with RER-positive tumors compared to RER-negative tumors. Patients with RER-positive tumors were found to have an improved prognosis: the 5-year survival probability for patients with RER-positive tumors was 68%, as compared to 32% for patients with RER-negative tumors (p < 0.05).
  
  
  CONCLUSIONS
  RER-positive tumors are common in young patients with CRC, and patients with RER-positive tumors have a significantly improved prognosis. Because of their young age, survival data and prognosis play an important role in the overall treatment plan of young patients with CRC. Therefore, knowledge of RER status could affect initial therapy, postoperative chemotherapy, and follow-up.
authors.affiliation: Department of Surgery, National Naval Medical Center, Bethesda, Maryland, USA.
authors.forename: J R
authors.initials: JR
authors.lastname: Lukish
authors.forename: K
authors.initials: K
authors.lastname: Muro
authors.forename: J
authors.initials: J
authors.lastname: DeNobile
authors.forename: R
authors.initials: R
authors.lastname: Katz
authors.forename: J
authors.initials: J
authors.lastname: Williams
authors.forename: D F
authors.initials: DF
authors.lastname: Cruess
authors.forename: W
authors.initials: W
authors.lastname: Drucker
authors.forename: I
authors.initials: I
authors.lastname: Kirsch
authors.forename: S R
authors.initials: SR
authors.lastname: Hamilton
mesh_terms: D000328:Adult; D017677:Age Distribution; D015179:Colorectal Neoplasms / Q000235:genetics* / Q000401:mortality / Q000473:pathology / Q000628:therapy; D004261:DNA Replication / Q000235:genetics*; D004273:DNA, Neoplasm / Q000235:genetics*; D018900:Dinucleotide Repeats; D005260:Female; D006801:Humans; D008297:Male; D009367:Neoplasm Staging; D016133:Polymerase Chain Reaction / Q000379:methods; D011237:Predictive Value of Tests; D011379:Prognosis; D012189:Retrospective Studies; D016019:Survival Analysis
chemical_list: D004273:DNA, Neoplasm