Changes in paired-pulse depression during the triggering of seizures by 2 Hz dentate gyrus stimulation: effect of the kindling.
In this study, seizures in the dentate gyrus were triggered by 2 Hz electrical stimulation while, at the same time, serial changes in paired-pulse depression was measured in free-moving rats. The perforant path was stimulated by paired pulses 25 ms apart and recordings were made in the dentate gyrus. We also observed the effect of kindling procedure on this change of evoked field potential. Paired-pulse depression started to fail following the onset of epileptic afterdischarge, both before and after kindling. As kindling progressed, paired-pulse depression increased in the initial part of the stimulus train, and also the time delay from the start of the stimulation to when paired-pulse depression started to fail increased significantly. These findings suggest that the collapse of local early, presumably GABAA-mediated, inhibition may occur after the dentate gyrus seizure onset and development of kindling epileptogenesis does not weaken but, on the contrary, enhances this local inhibitory function. Finally we propose that seizures triggered by 2 Hz electrical stimulation constitutes a useful model for examining physiological or biochemical changes during seizure initiation of awake, free-moving animals since it provides long (usually several seconds or more) latency from the start of stimulation to seizure onset.
Emori
K
K
Division of Cortical Function Disorder, National Institute of Neuroscience, NCNP, Tokyo, Japan.
Katsumori
H
H
Minabe
Y
Y
D000818:Animals; D018891:Dentate Gyrus / Q000503:physiopathology*; D004558:Electric Stimulation; D004569:Electroencephalography; D004827:Epilepsy / Q000503:physiopathology*; D007696:Kindling, Neurologic / Q000502:physiology*; D008297:Male; D009473:Neuronal Plasticity; D019580:Perforant Pathway / Q000503:physiopathology; D051381:Rats; D017208:Rats, Wistar