Selective enrichment in human megakaryocyte progenitors by the B203.13 surface differentiation antigen.
The B203.13 monoclonal antibody specifically recognizes a subset (8-18.5%) of human CD34+ haemopoietic progenitors, which are significantly (P<0.05) enriched in megakaryocyte progenitors (CFU-meg). Moreover B203.13 expression was progressively lost as maturation proceeded along the megakaryocytic lineage. In fact: (i) CFU-meg derived from CD34+/B203.13+ showed a greater number of cells/colony with respect to CD34+/B203.13-; (ii) after 10 d of liquid cultures with 100 ng/ml of thrombopoietin, only a minority of CD34-derived cells positive for CD41 coexpressed B203.13; (iii) in situ immunocytochemical staining revealed that B203.13 was expressed exclusively on immature megakaryoblasts; (iv) circulating platelets did not express B203.13.
Vitale
M
M
Department of Biomedical Sciences and Biotechnology, Human Anatomy Section, Brescia, Italy.
Zauli
G
G
Bassini
A
A
Visani
G
G
Rezzani
R
R
Croce
C M
CM
Manzoli
F A
FA
D000943:Antigens, Differentiation / Q000378:metabolism*; D001792:Blood Platelets / Q000276:immunology; D006412:Hematopoietic Stem Cells / Q000276:immunology*; D006801:Humans; D007150:Immunohistochemistry; D008533:Megakaryocytes / Q000276:immunology*
D000943:Antigens, Differentiation