Carbachol increases contractions and intracellular Ca++ transients in guinea pig ventricular myocytes.
We tested hypotheses concerning the muscarinic receptor subtype and the involvement of L-type Ca current (ICa) in the stimulation of contractions by carbachol (CCh) in single guinea pig ventricular myocytes. When superfused with Tyrode's solution (36 degrees C, 5.4 mM [Ca++]o) and stimulated at 0.2 Hz, CCh (EC50 approximately 18 microM) increased the early component of isotonic contractions by acting at muscarinic receptors indistinguishable from the M2 subtype because AF-DX 116 (M2-selective) was more potent than pirenzepine (M1-selective) as an antagonist of the CCh effect. Action potential duration decreased slightly and ICa was not increased when CCh increased contractions. Carbachol increased intracellular Ca++ transients and contractions reversibly, which indicated an effect via sarcoplasmic reticulum (SR) Ca stores. Ryanodine (1-10 microM) blocked the early contraction component increased by CCh, another indication that CCh action depends on SR Ca stores. We previously found that CCh increased a background Na+ current by occupancy of M2 receptors. We now report that the increased contractions by CCh can also originate at M2 receptors and that SR Ca stores are involved in the CCh effect. Because CCh did not significantly increase ICa, the initial increase of intracellular Na+ by CCh may eventually act through Na-Ca exchange to enhance excitation-contraction coupling.
Protas
L
L
Department of Pharmacology, University of Connecticut Health Center, Farmington, USA.
Shen
J B
JB
Pappano
A J
AJ
D000200:Action Potentials / Q000187:drug effects; D000818:Animals; D002118:Calcium / Q000378:metabolism*; D015220:Calcium Channels / Q000187:drug effects; D002217:Carbachol / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D005260:Female; D006168:Guinea Pigs; D008297:Male; D018721:Muscarinic Agonists / Q000494:pharmacology*; D009200:Myocardial Contraction / Q000187:drug effects*; D011493:Protein Kinase C / Q000502:physiology; D013696:Temperature
D015220:Calcium Channels; D018721:Muscarinic Agonists; D002217:Carbachol; D011493:Protein Kinase C; D002118:Calcium