title: Comparison of serotonin receptor numbers and activity in specific hypothalamic areas of sexually active and inactive female rats.
abstract: In a previous study we have shown that a positive correlation exists between 5-hydroxytryptamine (5-HT) activity and female sexual receptivity in the pre-optic area (POA) and median eminence (ME) and that there is a negative correlation in the ventromedial nucleus (VMN), zona incerta (ZI) and arcuate nucleus (ARC). In this report, the possibility that 5-HT receptor density and affinity alter with sexual receptivity has been investigated. Micropunches of the POA, VMN, ARC, ME and the anterior hypothalamus were dissected from ovariectomised rats primed with a submaximal steroid regime (2 microg oestradiol benzoate, OB, followed at 48 h by 0.05 mg progesterone) which induced receptivity (lordosis quotient, LQ, 80-100%) in approximately half the animals, the remaining half usually exhibiting an LQ <20%. Binding studies were carried out using 3H-ketanserin (5-HT2A ligand) and 3H-8-hydroxy-2-(di-n-propylamine)tetraline (8-OHDPAT; 5-HT1A ligand) and pooled (n = 5) micropunch samples. The Bmax of 5-HT2A receptors in the sexually receptive groups was significantly (p < 0.05) greater in the POA and ME and significantly lower in the VMN, ARC and ZI when compared with values in the non-receptive animals. The KD values of the 5-HT2A receptors did not differ in the two groups (except the ZI, where the KD was lower in receptive rats). Neither the Bmax nor KD of the 5-HT1A receptors differed in the two groups in any area investigated. Administration of the 5-HT2 agonists dimethoxyiodophenylaminopropane and m-chlorophenyl piperazine into the POA resulted in enhanced sexual activity in animals exhibiting a low level of receptivity, after 5 microg OB given alone while ketanserin (5-HT2A antagonist) in the POA inhibited sexual activity in receptive animals primed with the submaximal steroid regime given above. In the same models, neither the 5-HT2 agonists nor the 5-HT2A antagonist affected behaviour when applied to the VMN. The 5-HT1A agonist 8-OHDPAT exerted an inhibitory effect in the VMN. These findings, together with earlier results, show that in receptive animals there is an increase in both 5-HT turnover and 5-HT2A receptors in the POA and ME. Additionally 5-HT2 agonists and an antagonist applied to the POA enhance and reduce sexual activity, respectively. This suggests that the 5-HT2 system in the POA has a stimulatory role in the control of female sexual behaviour. Both 5-HT activity and 5-HT2 receptors are decreased in the VMN, ARC and ZI of receptive animals. 5-HT2 agonists and a 5-HT2A antagonist have no effect in the VMN indicating that there is no 5-HT2-stimulatory or -inhibitory effect in this area, at least in the animal models used in these experiments. However, the VMN is a site of an inhibitory action mediated by the 5-HT1A receptors.
authors.affiliation: Department of Obstetrics and Gynaecology, St. George's Hospital Medical School, London, UK.
authors.forename: M I
authors.initials: MI
authors.lastname: Gonzalez
authors.forename: P
authors.initials: P
authors.lastname: Greengrass
authors.forename: M
authors.initials: M
authors.lastname: Russell
authors.forename: C A
authors.initials: CA
authors.lastname: Wilson
mesh_terms: D000818:Animals; D004958:Estradiol / Q000494:pharmacology*; D005260:Female; D007031:Hypothalamus / Q000187:drug effects* / Q000378:metabolism; D010052:Ovariectomy; D010053:Ovary / Q000502:physiology; D011374:Progesterone / Q000494:pharmacology*; D051381:Rats; D017208:Rats, Wistar; D011985:Receptors, Serotonin / Q000187:drug effects* / Q000378:metabolism; D018490:Serotonin Agents / Q000494:pharmacology; D012726:Sexual Behavior, Animal / Q000502:physiology*
chemical_list: D011985:Receptors, Serotonin; D018490:Serotonin Agents; D011374:Progesterone; D004958:Estradiol