Ostertagia circumcincta: isolation of a partial cDNA encoding an unusual member of the mitochondrial processing peptidase subfamily of M16 metallopeptidases.
A reverse-transcriptase polymerase chain reaction (PCR) procedure was used to isolate an Ostertagia circumcincta partial cDNA encoding a protein with general primary sequence features characteristic of members of the mitochondrial processing peptidase (MPP) subfamily of M16 metallopeptidases. The structural relationships of the predicted protein (Oc MPPX) with MPP subfamily proteins from other species (including the model free-living nematode Caenorhabditis elegans) were examined, and Northern analysis confirmed the expression of the Oc mppx gene in adult nematodes.
Walker
J
J
Department of Veterinary Parasitology, University of Glasgow, U.K.
Tait
A
A
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics; D015801:Helminth Proteins / Q000145:classification / Q000235:genetics*; D008666:Metalloendopeptidases / Q000145:classification / Q000235:genetics*; D008928:Mitochondria / Q000201:enzymology* / Q000235:genetics; D008969:Molecular Sequence Data; D010028:Ostertagia / Q000201:enzymology / Q000235:genetics*; D011499:Protein Processing, Post-Translational; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D000096122:Mitochondrial Processing Peptidase
D018076:DNA, Complementary; D015801:Helminth Proteins; D008666:Metalloendopeptidases; D000096122:Mitochondrial Processing Peptidase