title: Growth hormone and Pit-1 expression in bovine fetal lymphoid cells.
abstract: Bovine fetal lymphoid cells were examined for growth hormone (GH) and the transcription factor Pit-1/GHF-1 mRNA. GH and Pit-1/GHF-1 transcripts were detected in thymocytes and splenocytes from fetuses at 60, 90, 120, and 270 d of gestation using reverse transcription-polymerase chain reaction (RT-PCR). Northern analysis indicated that the lymphoid GH mRNA was approximately 350 nucleotides larger than in the pituitary. RT-PCR analysis demonstrated that the coding regions as well as 3' untranslated region of the lymphocyte GH and pituitary transcripts were the same. Analysis of the 5'-untranslated region of the lymphocyte GH mRNA showed that transcription began upstream from the start site in the pituitary gland, suggesting differences in regulation in these tissues. Fetal thymocytes and splenocytes expressed Pit-1/GHF-1 mRNA; however, they contained only the 2.5-kb transcript. The GH and Pit-1/GHF-1 mRNA in fetal lymphoid cells supports the hypothesis that lymphocyte-derived GH may function as an autocrine and/or paracrine factor during the development and maturation of the bovine fetal immune system.
authors.affiliation: Department of Pathobiological Sciences, School of Veterinary Medicine, University of Wisconsin-Madison, USA.
authors.forename: H T
authors.initials: HT
authors.lastname: Chen
authors.forename: L A
authors.initials: LA
authors.lastname: Schuler
authors.forename: R D
authors.initials: RD
authors.lastname: Schultz
mesh_terms: D000818:Animals; D015152:Blotting, Northern; D002417:Cattle / Q000196:embryology*; D004268:DNA-Binding Proteins / Q000235:genetics*; D005260:Female; D015870:Gene Expression; D005865:Gestational Age; D013006:Growth Hormone / Q000235:genetics*; D008214:Lymphocytes / Q000737:chemistry*; D016133:Polymerase Chain Reaction; D011247:Pregnancy; D012333:RNA, Messenger / Q000032:analysis / Q000737:chemistry; D012194:RNA-Directed DNA Polymerase; D013154:Spleen / Q000196:embryology; D013950:Thymus Gland / Q000196:embryology; D050817:Transcription Factor Pit-1; D014157:Transcription Factors / Q000235:genetics*
chemical_list: D004268:DNA-Binding Proteins; D012333:RNA, Messenger; D050817:Transcription Factor Pit-1; D014157:Transcription Factors; D013006:Growth Hormone; D012194:RNA-Directed DNA Polymerase