title: A series of vectors to construct lacZ fusions for the study of gene expression in Schizosaccharomyces pombe.
abstract: We have constructed a series of plasmids to facilitate the fusion of promoters with or without coding regions of genes of Schizosaccharomyces pombe to the lacZ gene of Escherichia coli. These vectors carry a multiple cloning region in which fission yeast DNA may be inserted in three different reading frames with respect to the coding region of lacZ. The plasmids were constructed with the ura4+ or the his3+ marker of S. pombe. Functionality of the plasmids was tested measuring in parallel the expression of fructose 1,6-bisphosphatase and beta-galactosidase under the control of the fbp1+ promoter in different conditions.
authors.affiliation: Instituto de Investigaciones Biomédicas C.S.I.C. Unidad de Bioquímica y Genética de Levaduras, Madrid, Spain.
authors.forename: M J
authors.initials: MJ
authors.lastname: Lafuente
authors.forename: T
authors.initials: T
authors.lastname: Petit
authors.forename: C
authors.initials: C
authors.lastname: Gancedo
mesh_terms: D001483:Base Sequence; D003001:Cloning, Molecular / Q000379:methods; D004262:DNA Restriction Enzymes; D004926:Escherichia coli / Q000235:genetics; D015966:Gene Expression Regulation, Fungal; D005819:Genetic Markers; D005822:Genetic Vectors / Q000235:genetics*; D007763:Lac Operon / Q000235:genetics*; D008969:Molecular Sequence Data; D010957:Plasmids / Q000235:genetics; D011401:Promoter Regions, Genetic / Q000235:genetics; D016364:Reading Frames / Q000235:genetics; D011993:Recombinant Fusion Proteins / Q000235:genetics; D012568:Schizosaccharomyces / Q000235:genetics*
chemical_list: D005819:Genetic Markers; D011993:Recombinant Fusion Proteins; D004262:DNA Restriction Enzymes