Damaged chromatin does not prevent the exit from metaphase I in fused mouse oocytes.
The presence of checkpoint mechanisms which are able to recognize damaged chromatin and thereafter to prevent exit from metaphase I has been investigated in giant mouse oocytes produced by fusion of a normal metaphase I oocyte with an equivalent oocyte with damaged chromatin. The presence of damaged chromatin did not prevent the onset of anaphase I in both sets of chromatin in the fused cells. Interestingly, fused or unfused cells containing only damaged chromatin failed to enter anaphase and persisted instead in a metaphase-like state. These results demonstrate the fragility of checkpoint controls in mammalian female germ cells.
Fulka
J
J
Institute of Animal Production, Prague, Czech Republic.
Kalab
P
P
First
N L
NL
Moor
R M
RM
D000818:Animals; D002459:Cell Fusion; D002843:Chromatin / Q000473:pathology*; D005260:Female; D008677:Metaphase; D051379:Mice; D009865:Oocytes / Q000473:pathology*
D002843:Chromatin