Isolation by differential display and characterization of a tobacco auxin-responsive cDNA Nt-gh3, related to GH3.
By use of differential display, we have isolated a new early auxin-responsive cDNA in Nicotiana tabacum. Nt-gh3 had 70% identity with the unique GH3 sequence isolated in soybean by Hagen et al. [Planta 162 (1984) 147-153] and is thus the first reported cDNA related to this gene until now. Nt-gh3 mRNA accumulates within a short time after auxin treatment, responds to very low concentrations of NAA (as little as 10[-9] M) and specifically to active auxins. Nt-gh3 mRNA is demonstrated to be one of the most relevant early molecular markers of primary auxin response.
Roux
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Institut des Sciences Végétales, UPR 0040, CNRS, Gif-sur-Yvette, France.
Perrot-Rechenmann
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D000595:Amino Acid Sequence; D001483:Base Sequence; D003001:Cloning, Molecular; D003513:Cycloheximide / Q000494:pharmacology; D018076:DNA, Complementary / Q000235:genetics*; D018744:DNA, Plant / Q000235:genetics*; D018506:Gene Expression Regulation, Plant / Q000187:drug effects; D007210:Indoleacetic Acids / Q000494:pharmacology*; D008969:Molecular Sequence Data; D010940:Plant Proteins / Q000235:genetics*; D010947:Plants, Toxic; D016133:Polymerase Chain Reaction / Q000379:methods; D011500:Protein Synthesis Inhibitors / Q000494:pharmacology; D012333:RNA, Messenger / Q000096:biosynthesis; D018749:RNA, Plant / Q000096:biosynthesis; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D030262:Soybean Proteins; D014026:Nicotiana / Q000235:genetics*
D018076:DNA, Complementary; D018744:DNA, Plant; C070797:GH3 protein, soybean; D007210:Indoleacetic Acids; D010940:Plant Proteins; D011500:Protein Synthesis Inhibitors; D012333:RNA, Messenger; D018749:RNA, Plant; D030262:Soybean Proteins; D003513:Cycloheximide