The B cell-specific coactivator OBF-1 (OCA-B, Bob-1) is inducible in T cells and its expression is dispensable for IL-2 gene induction.
The transcriptional coactivator variously known as OBF-1, OCA-B or Bob-1 has previously been shown to be expressed in a highly B cell-specific manner. Here we show that expression of the OBF-1 gene is also observed in several T cell lines as well as in primary T cells following activation. This suggests that this coactivator may also be involved in transcriptional control in T lymphocytes. In addition we show that several potential T cell-specific target genes which have octamer sites in their regulatory regions, such as the genes coding for interleukin-2, -3 and -4, are still properly regulated in T cells isolated from OBF-1-/- mice.
Sauter
P
P
Friedrich Miescher Institute, Basel, Switzerland.
Matthias
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P
D000818:Animals; D001402:B-Lymphocytes / Q000378:metabolism*; D002460:Cell Line; D002478:Cells, Cultured; D015870:Gene Expression; D007376:Interleukin-2 / Q000096:biosynthesis / Q000235:genetics*; D007377:Interleukin-3 / Q000096:biosynthesis / Q000235:genetics; D015847:Interleukin-4 / Q000096:biosynthesis / Q000235:genetics; D051379:Mice; D018345:Mice, Knockout; D013601:T-Lymphocytes / Q000378:metabolism*; D015534:Trans-Activators / Q000096:biosynthesis* / Q000235:genetics
D007376:Interleukin-2; D007377:Interleukin-3; C490424:Pou2af1 protein, mouse; D015534:Trans-Activators; D015847:Interleukin-4