The 8S benzo(a)pyrene-binding protein is an aldehyde dehydrogenase regulated by the Ah receptor.
8S Benzo(a)pyrene-binding proteins from liver cytosol of mouse and rabbit have been partially purified by gel permeation chromatography and affinity chromatography on 1-aminopyrene-Sepharose columns. These proteins, which bind polycyclic aromatic hydrocarbons and daunorubicine, have been identified, by microsequencing, as aldehyde dehydrogenases composed of polypeptides of 54 kDa. Using Ah receptor-deficient (AHR-/-) transgenic mice it has been shown that the amount as well as the binding capabilities of 8S protein was strongly altered in these mice, suggesting that its expression was partially under the control of the Ah receptor. The function of these proteins is currently unknown.
Lesca
P
P
Laboratoire de Pharmacologie-Toxicologie, INRA, Toulouse, France.
Pineau
T
T
Galtier
P
P
Peryt
B
B
Derancourt
J
J
D000444:Aldehyde Dehydrogenase / Q000096:biosynthesis* / Q000737:chemistry; D000080924:Aldehyde Dehydrogenase 1 Family; D000595:Amino Acid Sequence; D000818:Animals; D001667:Binding, Competitive; D002352:Carrier Proteins / Q000096:biosynthesis* / Q000737:chemistry; D003630:Daunorubicin / Q000378:metabolism; D015971:Gene Expression Regulation, Enzymologic; D006168:Guinea Pigs; D008099:Liver / Q000201:enzymology; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D008817:Mice, Mutant Strains; D008822:Mice, Transgenic; D008969:Molecular Sequence Data; D011084:Polycyclic Aromatic Hydrocarbons / Q000378:metabolism; D011721:Pyrenes / Q000378:metabolism; D011817:Rabbits; D018336:Receptors, Aryl Hydrocarbon / Q000172:deficiency / Q000235:genetics / Q000378:metabolism*; D050697:Retinal Dehydrogenase; D012441:Saccharomyces cerevisiae / Q000201:enzymology; D017421:Sequence Analysis; D013045:Species Specificity
D002352:Carrier Proteins; D011084:Polycyclic Aromatic Hydrocarbons; D011721:Pyrenes; D018336:Receptors, Aryl Hydrocarbon; C032445:benzopyrene carrier protein, mouse; D000080924:Aldehyde Dehydrogenase 1 Family; D000444:Aldehyde Dehydrogenase; C515028:ALDH1A1 protein, mouse; D050697:Retinal Dehydrogenase; D003630:Daunorubicin