title: Detection of serum antibodies against phosphocholine-containing aminoglycoglycerolipid specific to Mycoplasma fermentans in HIV-1 infected individuals.
abstract: Mycoplasma fermentans has been associated with acquired immunodeficiency syndrome (AIDS). The lipids extracted from five strains of M. fermentans and eight other species of mycoplasmas were investigated. By using high-performance thin-layer chromatography (HPTLC) and immunostaining on HPTLC-plates with polyclonal and monoclonal antibodies against lipids of M. fermentans, a glycophospholipid GGPL-III was proved to be a specific lipid and important antigen of the species of M. fermentans. Previously we reported that M. fermentans had phosphocholine-containing glycoglycerolipid (GGPL-I and GGPL-III). TLC immunostaining showed that anti-GGPL-III specific antibodies were detected in HIV-1 infected individuals. By employing purified GGPL-III, an enzyme-linked immunosorbent assay (ELISA) has been developed for the detection of specific antibodies to GGPL-III in human serum. This ELISA showed high sensitivity, specificity and good reproducibility. Frequency of antibodies to GGPL-III was significantly higher in sera from HIV-1 infected people (44.6%, 29/65) than from HIV-1 negative healthy donors (1.7%, 2/117) or patients with three kinds of other diseases we investigated (0-8.7%, 0/20-2/23). Thus M. fermentans infection seemed to be correlated with HIV-1 infection although further investigation is essential for clarification of the role of M. fermentans during the long latency period in HIV infected individuals.
authors.affiliation: Department of Microbiology, Tokyo Medical and Dental University, Japan.
authors.forename: J L
authors.initials: JL
authors.lastname: Li
authors.forename: K
authors.initials: K
authors.lastname: Matsuda
authors.forename: M
authors.initials: M
authors.lastname: Takagi
authors.forename: N
authors.initials: N
authors.lastname: Yamamoto
mesh_terms: D000907:Antibodies, Bacterial / Q000097:blood*; D000918:Antibody Specificity; D000942:Antigens, Bacterial / Q000276:immunology; D004797:Enzyme-Linked Immunosorbent Assay; D006017:Glycolipids / Q000276:immunology*; D015658:HIV Infections / Q000150:complications; D015497:HIV-1; D006801:Humans; D009175:Mycoplasma Infections / Q000097:blood / Q000150:complications; D016858:Mycoplasma fermentans / Q000145:classification / Q000276:immunology*; D010743:Phospholipids / Q000276:immunology*; D010767:Phosphorylcholine / Q000276:immunology; D013045:Species Specificity
chemical_list: C106372:1''-phosphocholine-2''-aminodihydroxypropane-3''-phospho-6'-glucopyranosyl-(1'-3)-1,2-dipalmitoylglycerol; D000907:Antibodies, Bacterial; D000942:Antigens, Bacterial; D006017:Glycolipids; D010743:Phospholipids; D010767:Phosphorylcholine