Action of FMRFamide-like peptides on porcine gastrointestinal motility in vitro.
Mechanical activity was recorded in circular and longitudinal smooth muscle preparations isolated from extensive regions of the porcine gastrointestinal tract in response to the FMRFamide-like neuropeptides F8Famide and A18Famide. In all preparations, the peptides were about equipotent in producing phasic contractions or enhancing spontaneous activity. The most prominent responses were observed in jejunal longitudinal strips which were on the average 91% (+/- 4% SEM, n = 15; 10(-6) M) of the histamine (10(-5) M) responses. The peptide-induced phasic activity was completely abolished by nifedipine but was unaffected by tetrodotoxin, atropine, phentolamine, yohimbine, phenoxybenzamine, propranolol, methysergide, cimetidine, indomethacin, levallorphane or naloxone. Both peptides enhanced acetylcholine-induced contractions. However, bovine ileum and guinea-pig taenia coli was not affected by these peptides. The results indicate that F8F- and A18F-amide contract porcine gastrointestinal smooth muscle by acting directly via non-opioid receptors on L-type calcium channels. In addition an increase of the sensitivity to cholinergic stimulation occurs.
Decker
B
B
Department of Physiology, University of Marburg, Marburg/Lahn, Germany.
Vadokas
B
B
Kutschenreuter
U
U
Golenhofen
K
K
Voigt
K
K
McGregor
G P
GP
Mandrek
K
K
D000818:Animals; D015220:Calcium Channels / Q000378:metabolism; D020746:Calcium Channels, L-Type; D004743:Enkephalin, Leucine / Q000494:pharmacology; D004744:Enkephalin, Methionine / Q000494:pharmacology; D019835:FMRFamide / Q000031:analogs & derivatives / Q000494:pharmacology*; D005769:Gastrointestinal Motility / Q000187:drug effects*; D006632:Histamine / Q000494:pharmacology; D066298:In Vitro Techniques; D009119:Muscle Contraction / Q000187:drug effects; D009130:Muscle, Smooth / Q000187:drug effects / Q000502:physiology; D009479:Neuropeptides / Q000737:chemistry / Q000494:pharmacology*; D009543:Nifedipine / Q000494:pharmacology; D009599:Nitroprusside / Q000494:pharmacology; D009842:Oligopeptides / Q000494:pharmacology*; D013552:Swine
D015220:Calcium Channels; D020746:Calcium Channels, L-Type; D009479:Neuropeptides; D009842:Oligopeptides; D009599:Nitroprusside; D004744:Enkephalin, Methionine; D004743:Enkephalin, Leucine; D019835:FMRFamide; D006632:Histamine; C053651:phenylalanyl-leucyl-phenylalanyl-glutaminyl-prolyl-glutaminyl-arginyl-phenylalaninamide; C061209:A18Famide; D009543:Nifedipine