Cytogenetic alterations in eight mammary tumors and tumor-suppressor gene p53 mutation in one mammary tumor from dogs.
OBJECTIVE
To identify mutations in canine mammary tumors.


METHODS
10 tumor-bearing dogs.


METHODS
Culture of neoplastic cells originating from mammary tumors was performed, and trypsin-G banding was used for cytogenetic investigations. The same tumors were subjected to molecular genetic screening by use of DNA extraction, polymerase chain reaction, DNA elution, and DNA sequencing for ras oncogenes and the p53 tumor suppressor gene.


RESULTS
A broad spectrum of chromosome aberrations was observed, including trisomies, reciprocal translocations, and structural and numerical X-chromosome alterations and deletions. Molecular genetic analysis revealed a tumor suppressor p53 gene mutation in codon 249 of exon 7 in one instance. Interestingly, analyzed mammary tumors were free of mutations in N-ras, K-ras and H-ras, exons 1 and 2.


CONCLUSIONS
Chromosome alterations are wide-spread in canine mammary tumors, but no ras family mutations were detected in tumors from these 10 dogs.


CONCLUSIONS
Knowledge about chromosome, oncogene, and tumor suppressor gene damage could be helpful for diagnosis and prognosis of neoplastic diseases in dogs.
Mayr
B
B
Institute for Animal Breeding and Genetics, Veterinary University, Vienna, Austria.
Dressler
A
A
Reifinger
M
M
Feil
C
C
D000230:Adenocarcinoma / Q000235:genetics / Q000473:pathology / Q000662:veterinary*; D000818:Animals; D002277:Carcinoma / Q000235:genetics / Q000473:pathology / Q000662:veterinary; D002869:Chromosome Aberrations; D004273:DNA, Neoplasm / Q000032:analysis; D004283:Dog Diseases; D004285:Dogs; D005091:Exons; D005260:Female; D017353:Gene Deletion; D016158:Genes, p53; D011905:Genes, ras; D007621:Karyotyping; D015674:Mammary Neoplasms, Animal / Q000235:genetics* / Q000473:pathology*; D009154:Mutation; D016133:Polymerase Chain Reaction; D012729:Sex Chromosome Aberrations; D014178:Translocation, Genetic; D014314:Trisomy; D014407:Tumor Cells, Cultured; D014960:X Chromosome
D004273:DNA, Neoplasm