title: Immunohistolocalization of carbonic anhydrase isozymes (CA-I, CA-II and CA-III) in bovine male reproductive tracts.
abstract: Immunohistochemical localizations of carbonic anhydrase isozymes (CA-I, CA-II and CA-III) in bovine male reproductive tracts were studied. In bulls, no immunoreaction was seen after treatment with antibodies to CA-I, -II and CA-III in the testis. Specific staining for CA-III, however, was evident in peritubular cells in interstitial tissue of the testis, epididymis. CA-II activity could be detected in epithelium of the epididymis, ductus deferentis and ampulla ductus deferentis. Especially, a strong reaction for CA-II was seen in apical in epithelium of the epididymis in the initial and middle segment. CA-I activity was only founded in ductus deferentis and ampulla ductus deferentis. No or a weak reaction for CA-I, CA-II and CA-III were seen in the three accessory reproductive glands. Specific immunostaining for CA-II and CA-I could be observed in the organ, suggesting the bicarbonate in bovine semen to derive primarily from the genital tract and not accessory reproductive organs. CA-III-positive peritubular cells in interstitial tissue were also stained for alpha smooth muscle actin, and were very similar to contractile myofibroblast cells (Wrobel et al., 1979).
authors.affiliation: Department of Animal Models for Human Disease, National Institute of Neuroscience, National Center of Neurology and Psychiatry, Tokyo, Japan.
authors.forename: N
authors.initials: N
authors.lastname: Ichihara
authors.forename: K
authors.initials: K
authors.lastname: Sasaki
authors.forename: S
authors.initials: S
authors.lastname: Iseri
authors.forename: H
authors.initials: H
authors.lastname: Kimura
authors.forename: K
authors.initials: K
authors.lastname: Miura
authors.forename: T
authors.initials: T
authors.lastname: Nishita
authors.forename: M
authors.initials: M
authors.lastname: Asari
mesh_terms: D000185:Actinin / Q000378:metabolism; D000818:Animals; D000918:Antibody Specificity; D002256:Carbonic Anhydrases / Q000378:metabolism*; D002417:Cattle; D003600:Cytosol / Q000201:enzymology*; D005837:Genitalia, Male / Q000201:enzymology*; D007150:Immunohistochemistry; D007527:Isoenzymes / Q000378:metabolism*; D007985:Leydig Cells / Q000201:enzymology; D008297:Male
chemical_list: D007527:Isoenzymes; D000185:Actinin; D002256:Carbonic Anhydrases