title: Heat shock and proinflammatory stressors induce differential localization of heat shock proteins in human monocytes.
abstract: Heat shock (HS) proteins (HSP) are a family of molecular chaperones induced by environmental stresses such as oxidative injury, and contribute to protection from and adaptation to cellular stress. We investigated in human monocytes the expression and subcellular distribution of hsp70 and hsc70 after HS and inflammation-related stresses leading to generation of reactive oxygen species by these cells, such as the phorbol ester PMA and erythrophagocytosis (E phi). By combining immunofluorescent staining and Western blot on subcellular fractions, we found that all three stress factors resulted in an increased hsp70 expression, however the subcellular distribution pattern was different depending on the type of stress. While HS induced a rapid translocation of hsp70 into the nucleus, no nuclear translocation of hsp70 was observed after PMA or E phi. Neither of the examined stresses induced membrane expression of hsp70. The observed differences in subcellular distribution pattern might relate to distinct regulation and specific functions of hsp70 in inflammation.
authors.affiliation: Laboratoire de Physiologie Respiratoire, UFR Cochin Port-Royal, Université Paris V, France.
authors.forename: E
authors.initials: E
authors.lastname: Mariéthoz
authors.forename: M R
authors.initials: MR
authors.lastname: Jacquier-Sarlin
authors.forename: G
authors.initials: G
authors.lastname: Multhoff
authors.forename: A M
authors.initials: AM
authors.lastname: Healy
authors.forename: F
authors.initials: F
authors.lastname: Tacchini-Cottier
authors.forename: B S
authors.initials: BS
authors.lastname: Polla
mesh_terms: D001692:Biological Transport; D002451:Cell Compartmentation; D002478:Cells, Cultured; D018882:Heat Stress Disorders; D006360:Heat-Shock Proteins / Q000032:analysis / Q000378:metabolism*; D006801:Humans; D007249:Inflammation; D009000:Monocytes / Q000166:cytology / Q000378:metabolism*; D017382:Reactive Oxygen Species
chemical_list: D006360:Heat-Shock Proteins; D017382:Reactive Oxygen Species