title: The influence of oral glucose intake on binding and degradation of 125I-insulin by receptors on erythrocytes as well as on insulin and C-peptide serum levels in patients after myocardial infarction and healthy individuals.
abstract: In this study, we investigated the influence of glucose administration on binding and degradation of 125I-insulin by receptors on erythrocytes as well as on insulin and C-peptide serum levels in 15 patients after myocardial infarction and in 15 age-matched healthy persons. Venous blood samples were taken directly before and at 30, 60 and 120 minutes after oral administration of 75 g of glucose. In the collected blood samples serum glucose, insulin and C-peptide levels were determined. Binding and degradation of 125I-insulin by specific receptors on red blood cells were evaluated using the method described by Gambhir and modified by the authors. Serum insulin and C-peptide levels were significantly higher while binding of 125I-insulin to erythrocytes was decreased in patients after myocardial infarction. These results seem to support the hypothesis that insulin resistance and hyperinsulinism play a role in the pathogenesis of ischaemic heart disease. Impaired degradation of 125I-insulin during the oral glucose tolerance test in the patients after myocardial infarction indicates that insulin resistance is located at the receptor level.
authors.affiliation: Chair of Physiology, Biochemistry and Hygiene, University School of Physical Education, Poznań, Poland.
authors.forename: T
authors.initials: T
authors.lastname: Rychlewski
authors.forename: L
authors.initials: L
authors.lastname: Szcześniak
authors.forename: P
authors.initials: P
authors.lastname: Dylewicz
authors.forename: E
authors.initials: E
authors.lastname: Deskur
authors.forename: I
authors.initials: I
authors.lastname: Przywarska
authors.forename: Z
authors.initials: Z
authors.lastname: Kasprzak
authors.forename: J
authors.initials: J
authors.lastname: Karolkiewicz
authors.forename: L
authors.initials: L
authors.lastname: Konys
mesh_terms: D000328:Adult; D002096:C-Peptide / Q000097:blood*; D004912:Erythrocytes / Q000378:metabolism*; D005947:Glucose / Q000494:pharmacology*; D005951:Glucose Tolerance Test; D006801:Humans; D007328:Insulin / Q000097:blood*; D007457:Iodine Radioisotopes; D008875:Middle Aged; D009203:Myocardial Infarction / Q000097:blood*
chemical_list: D002096:C-Peptide; D007328:Insulin; D007457:Iodine Radioisotopes; D005947:Glucose