title: Trinucleotide microsatellite loci and increased heterozygosity in cross-species applications in the social wasp, Polistes.
abstract: Though microsatellite loci are usually found to be most polymorphic in the species in which they are first identified, we have found significant increases in polymorphisms in some cross-species applications. We present eight new trinucleotide microsatellite loci derived from two species of social wasps, Polistes annularis and Polistes bellicosus. We assessed the primers designed from these species and the degree of polymorphism in two additional species, P. dorsalis, which is very closely related to P. bellicosus, and P. dominulus, which is an Old World congener, thought to have diverged from New World Polistes over 80 million years ago. Cross-species applications for these microsatellite loci indicate that the priming sites from P. bellicosus loci are conserved in P. dorsalis and amplified similarly sized products with higher heterozygosities than the original species in two of three cases. A locus that was monomorphic in P. annularis had a heterozygosity of 1.0 in the distantly related P. dominulus. Cross-species applications of these loci indicated that alleles were generally of similar lengths in the new and original species when they retained their heterozygosity.
authors.affiliation: Department of Ecology and Evolutionary Biology, Rice University, Houston, Texas 77005-1892, USA.
authors.forename: J E
authors.initials: JE
authors.lastname: Strassmann
authors.forename: J M
authors.initials: JM
authors.lastname: Peters
authors.forename: K
authors.initials: K
authors.lastname: Barefield
authors.forename: C R
authors.initials: CR
authors.lastname: Solís
authors.forename: C R
authors.initials: CR
authors.lastname: Hughes
authors.forename: D C
authors.initials: DC
authors.lastname: Queller
mesh_terms: D000483:Alleles; D000818:Animals; D017931:DNA Primers; D006579:Heterozygote; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic / Q000235:genetics*; D017422:Sequence Analysis, DNA; D018911:Trinucleotide Repeats / Q000235:genetics*; D014863:Wasps / Q000235:genetics*
chemical_list: D017931:DNA Primers