title: Structure and organization of the porcine LCN1 gene encoding Tear lipocalin/von Ebner's gland protein.
abstract: We have cloned and sequenced the entire genomic fragment of the porcine LCN1 gene which encodes Tear lipocalin/von Ebner's gland protein, a member of the lipocalin superfamily highly expressed in porcine lachrymal and lingual glands. The porcine LCN1 gene is approximately 4.6 kb in size and contains six protein-coding exons and a 3'-nontranslated exon. The structure of this porcine gene is highly similar, in terms of numbers of exons/introns, in size of exons and in intron phasing, to that of the human LCN1 and rat VEGP genes, thus supporting a very close evolutionary relationship of these genes. Within the promoter region of the porcine LCN1 a putative TATA box, a CAAT box and two MRE motifs are found. The same MRE motifs are conserved in the human LCN1 promoter, suggesting that they might be of relevance for LCN1 gene expression. However, additional motifs present in the human LCN1 promoter, such as AP-1 and AP-2 sites, a NF-kappaB site, and a cAMP-responsive element, could not be detected in the porcine LCN1 promoter.
authors.affiliation: Institut für Mikrobiologie (Medizinische Fakultät), Universität Innsbruck, Austria.
authors.forename: P
authors.initials: P
authors.lastname: Holzfeind
authors.forename: P
authors.initials: P
authors.lastname: Merschak
authors.forename: P
authors.initials: P
authors.lastname: Wojnar
authors.forename: B
authors.initials: B
authors.lastname: Redl
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002352:Carrier Proteins / Q000737:chemistry* / Q000235:genetics* / Q000302:isolation & purification; D006801:Humans; D007438:Introns; D054835:Lipocalin 1; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D051381:Rats; D012045:Regulatory Sequences, Nucleic Acid; D012689:Sequence Homology, Nucleic Acid; D013552:Swine / Q000235:genetics*
chemical_list: D002352:Carrier Proteins; C518443:LCN1 protein, human; C518449:Lcn1 protein, rat; D054835:Lipocalin 1