Nitric oxide synthase-, N-methyl-D-aspartate receptor-, glutamate- and aspartate-immunoreactive neurons in the mouse arcuate nucleus: effects of neonatal treatment with monosodium glutamate.
Glutamate-, aspartate-, N-methyl-D-aspartate receptor (NMDAR1 and 2 subunits)-, and nitric oxide synthase (NOS)-immunoreactive neurons were studied in the arcuate nucleus (AN) of mice treated neonatally with monosodium glutamate (MSG) which is known to cause extensive neuronal loss in this hypothalamic nucleus. It was found that intensely stained glutamate- and aspartate-immunoreactive neurons present in the AN of control mice were completely absent in the MSG-lesioned AN as well as the ventromedial nucleus lateral to the AN. Similarly, NMDAR1-immunoreactive neurons were mostly absent in the MSG-lesioned AN but remained intact in the ventromedial nucleus. There was also a substantial loss of NMDAR2 immunoreactivity within the AN. In contrast, NOS-immunoreactive neurons in the AN survived the neonatal glutamate treatment, although they appeared to be less intensely stained.
Xue
Y D
YD
Department of Pharmacology, Faculty of Medicine, National University of Singapore.
Wong
P T
PT
Leong
S K
SK
D000818:Animals; D000831:Animals, Newborn; D001111:Arcuate Nucleus of Hypothalamus / Q000166:cytology / Q000187:drug effects / Q000201:enzymology / Q000378:metabolism*; D001224:Aspartic Acid / Q000378:metabolism*; D005456:Fluorescent Dyes; D018698:Glutamic Acid / Q000378:metabolism*; D007150:Immunohistochemistry; D008297:Male; D051379:Mice; D009474:Neurons / Q000201:enzymology / Q000378:metabolism*; D019001:Nitric Oxide Synthase / Q000378:metabolism*; D010078:Oxazines; D016194:Receptors, N-Methyl-D-Aspartate / Q000378:metabolism*; D012970:Sodium Glutamate / Q000633:toxicity*
D005456:Fluorescent Dyes; D010078:Oxazines; D016194:Receptors, N-Methyl-D-Aspartate; C008619:Nile Blue; D001224:Aspartic Acid; D018698:Glutamic Acid; D019001:Nitric Oxide Synthase; D012970:Sodium Glutamate