Excision-repair patch lengths are similar for transcription-coupled repair and global genome repair in UV-irradiated human cells.
We have used the buoyant density shift method to measure excision-repair patch lengths in UV-irradiated repair-proficient human cells and in primary fibroblasts belonging to xeroderma pigmentosum complementation group C (XP-C), in which excision repair of UV-induced photoproducts is dependent upon transcription. The patch size was found to be about 30 nucleotides for both cell types. This agrees with the size of the DNA fragments excised in vitro by the dual incisions of the structure-specific nucleases XPG and ERCC1-XPF. We conclude that the XPC protein is not required to target the excision nucleases to sites of DNA cleavage in transcribed strands of expressed genes or to protect the newly incised DNA from further processing by exonucleases.
Bowman
K K
KK
Department of Biological Sciences, Stanford University, CA 94305-5020, USA. kbowman@leland.stanford.edu
Smith
C A
CA
Hanawalt
P C
PC
D002460:Cell Line; D004247:DNA / Q000737:chemistry / Q000235:genetics*; D004260:DNA Repair / Q000235:genetics*; D005347:Fibroblasts; D005354:Fibrosarcoma; D006801:Humans; D008970:Molecular Weight; D011740:Pyrimidine Dimers; D013762:Tetrahydrofolate Dehydrogenase / Q000235:genetics; D014158:Transcription, Genetic; D014407:Tumor Cells, Cultured; D014466:Ultraviolet Rays; D014983:Xeroderma Pigmentosum / Q000235:genetics
D011740:Pyrimidine Dimers; D004247:DNA; D013762:Tetrahydrofolate Dehydrogenase