Interleukin 10 increases CCR5 expression and HIV infection in human monocytes.
The immunosuppressive and antiinflammatory cytokine interleukin (IL) 10 selectively upregulates the expression of the CC chemokine receptors CCR5, 2, and 1 in human monocytes by prolonging their mRNA half-life. IL-10-stimulated monocytes display an increased number of cell surface receptors for, and better chemotactic responsiveness to, relevant agonists than do control cells. In addition, IL-10-stimulated monocytes are more efficiently infected by HIV BaL. This effect was associated to the enhancement of viral entry through CCR5. These data add support to an emerging paradigm in which pro- and antiinflammatory molecules exert reciprocal and opposing influence on chemokine agonist production and receptor expression.
Sozzani
S
S
Istituto di Ricerche Farmacologiche 'Mario Negri', 20157 Milan, Italy.
Ghezzi
S
S
Iannolo
G
G
Luini
W
W
Borsatti
A
A
Polentarutti
N
N
Sica
A
A
Locati
M
M
Mackay
C
C
Wells
T N
TN
Biswas
P
P
Vicenzi
E
E
Poli
G
G
Mantovani
A
A
D015152:Blotting, Northern; D004279:DNA, Viral / Q000378:metabolism; D005434:Flow Cytometry; D005786:Gene Expression Regulation / Q000235:genetics; D015658:HIV Infections / Q000821:virology*; D006801:Humans; D016753:Interleukin-10 / Q000494:pharmacology*; D007700:Kinetics; D009000:Monocytes / Q000821:virology*; D012333:RNA, Messenger / Q000187:drug effects / Q000378:metabolism; D054389:Receptors, CCR1; D019713:Receptors, CCR5 / Q000235:genetics / Q000378:metabolism*; D019707:Receptors, Chemokine / Q000235:genetics; D015854:Up-Regulation / Q000187:drug effects
C515300:CCR1 protein, human; D004279:DNA, Viral; D012333:RNA, Messenger; D054389:Receptors, CCR1; D019713:Receptors, CCR5; D019707:Receptors, Chemokine; D016753:Interleukin-10