Development and validation of a method to extract and quantitate paromomycin and gentamicin from an Aquaphilic cream formulation.
Butanol and dilute sulfuric acid were used to extract paromomycin and gentamicin from Aquaphilic-based formulated creams. The extraction procedure was validated over different antibiotic concentration ranges for linearity, precision, accuracy, limited specificity, sensitivity and solution stability.
Pick
J
J
SRI International, Life Sciences Division, Menlo Park, CA 94025, USA.
Olson
L L
LL
Ellis
W Y
WY
Lim
P
P
D020001:1-Butanol; D002626:Chemistry, Pharmaceutical; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D005839:Gentamicins / Q000032:analysis* / Q000302:isolation & purification; D009824:Ointments; D010303:Paromomycin / Q000032:analysis* / Q000302:isolation & purification; D011786:Quality Control; D013050:Spectrometry, Fluorescence; D013464:Sulfuric Acids
D005839:Gentamicins; D009824:Ointments; D013464:Sulfuric Acids; D010303:Paromomycin; D020001:1-Butanol; C033158:sulfuric acid