title: A method for measuring nitric oxide radical scavenging activity. Scavenging properties of sulfur-containing compounds.
abstract: A new method for the quantification of the nitric oxide (.NO) scavenging activity of compounds in aqueous solutions is described using an amperometric .NO sensor. After correction for the spontaneous degradation of .NO, second-order rate kinetics of the scavenging reaction are observe. The rate constant for hemoglobin found with this method is comparable with that found with an established spectrophotometric method. To demonstrate the capability of the method, several sulfur-containing compounds were tested (GSH, GSSG, S-methyl glutathione, N-acetyl cysteine, lipoic acid and dihydrolipoic acid). Of these compounds, only those that contained a thiol group displayed a considerable .NO scavenging activity.
authors.affiliation: Leiden/Amsterdam Center for Drug Research, Department of Pharmacochemistry, Faculty of Chemistry, Vrije Universiteit, The Netherlands.
authors.forename: M F
authors.initials: MF
authors.lastname: Vriesman
authors.forename: G R
authors.initials: GR
authors.lastname: Haenen
authors.forename: G J
authors.initials: GJ
authors.lastname: Westerveld
authors.forename: J B
authors.initials: JB
authors.lastname: Paquay
authors.forename: H P
authors.initials: HP
authors.lastname: Voss
authors.forename: A
authors.initials: A
authors.lastname: Bast
mesh_terms: D004563:Electrochemistry; D016166:Free Radical Scavengers / Q000737:chemistry*; D005609:Free Radicals / Q000032:analysis; D007700:Kinetics; D009569:Nitric Oxide / Q000032:analysis* / Q000737:chemistry; D013438:Sulfhydryl Compounds / Q000737:chemistry; D013457:Sulfur Compounds / Q000737:chemistry*
chemical_list: D016166:Free Radical Scavengers; D005609:Free Radicals; D013438:Sulfhydryl Compounds; D013457:Sulfur Compounds; D009569:Nitric Oxide