HNF-6 is expressed in endoderm derivatives and nervous system of the mouse embryo and participates to the cross-regulatory network of liver-enriched transcription factors.
Hepatocyte nuclear factor-6 (HNF-6) is a liver-enriched transcription factor that contains a single cut domain and a novel type of homeodomain. Here we have studied the developmental expression pattern of HNF-6 in the mouse. In situ hybridization experiments showed that HNF-6 mRNA is detected in the liver at embryonic day (E) 9, at the onset of liver differentiation. HNF-6 mRNA disappeared transiently from the liver between E12.5 and E15. In transfection experiments HNF-6 stimulated the expression of HNF-4 and of HNF-3 beta, two transcription factors known to be involved in liver development and differentiation. HNF-6 was detected in the pancreas from E10.5 onward, where it was restricted to the exocrine cells. HNF-6 was also detected in the developing nervous system. Both the brain and the spinal cord started to express HNF-6 at E9-9.5 in postmitotic neuroblasts. Later on, HNF-6 was restricted to brain nuclei, to the retina, to the ventral horn of the spinal cord, and to dorsal root ganglia. Our observations that HNF-6 contributes to the control of the expression of transcription factors and is expressed at early stages of liver, pancreas, and neuronal differentiation suggest that HNF-6 regulates several developmental programs.
Landry
C
C
Hormone and Metabolic Research Unit, International Institute of Cellular and Molecular Pathology, Brussels, Belgium.
Clotman
F
F
Hioki
T
T
Oda
H
H
Picard
J J
JJ
Lemaigre
F P
FP
Rousseau
G G
GG
D000818:Animals; D051778:Basic Helix-Loop-Helix Leucine Zipper Transcription Factors; D006528:Carcinoma, Hepatocellular / Q000473:pathology; D002454:Cell Differentiation / Q000235:genetics; D002490:Central Nervous System / Q000196:embryology* / Q000378:metabolism; D004268:DNA-Binding Proteins / Q000096:biosynthesis / Q000235:genetics; D005314:Embryonic and Fetal Development / Q000235:genetics; D004707:Endoderm / Q000378:metabolism*; D005136:Eye Proteins / Q000096:biosynthesis / Q000235:genetics; D005326:Fetal Proteins / Q000096:biosynthesis* / Q000235:genetics; D005347:Fibroblasts; D005727:Ganglia, Spinal / Q000196:embryology / Q000378:metabolism; D018507:Gene Expression Regulation, Developmental; D005865:Gestational Age; D051542:Hepatocyte Nuclear Factor 3-beta; D051557:Hepatocyte Nuclear Factor 4; D051559:Hepatocyte Nuclear Factor 6; D018398:Homeodomain Proteins / Q000096:biosynthesis* / Q000235:genetics; D006801:Humans; D017403:In Situ Hybridization; D008099:Liver / Q000196:embryology* / Q000378:metabolism; D008113:Liver Neoplasms / Q000473:pathology; D051379:Mice; D008815:Mice, Inbred Strains; D009419:Nerve Tissue Proteins / Q000096:biosynthesis* / Q000235:genetics; D009687:Nuclear Proteins / Q000096:biosynthesis / Q000235:genetics; D010179:Pancreas / Q000196:embryology / Q000378:metabolism; D010750:Phosphoproteins / Q000096:biosynthesis / Q000235:genetics; D051381:Rats; D012160:Retina / Q000196:embryology / Q000378:metabolism; D015534:Trans-Activators / Q000096:biosynthesis* / Q000235:genetics; D014157:Transcription Factors / Q000096:biosynthesis / Q000235:genetics; D014407:Tumor Cells, Cultured
D051778:Basic Helix-Loop-Helix Leucine Zipper Transcription Factors; D004268:DNA-Binding Proteins; D005136:Eye Proteins; C496502:FOXA2 protein, human; D005326:Fetal Proteins; C496503:Foxa2 protein, mouse; C496504:Foxa2 protein, rat; D051557:Hepatocyte Nuclear Factor 4; D051559:Hepatocyte Nuclear Factor 6; D018398:Homeodomain Proteins; C403128:MLX protein, human; D009419:Nerve Tissue Proteins; D009687:Nuclear Proteins; C496605:ONECUT1 protein, human; C496606:Onecut1 protein, mouse; C496607:Onecut1 protein, rat; D010750:Phosphoproteins; C501970:Tcfl4 protein, mouse; D015534:Trans-Activators; D014157:Transcription Factors; D051542:Hepatocyte Nuclear Factor 3-beta