title: Determination of cis-thymine glycol in DNA by gas chromatography-mass spectrometry with selected ion recording and multiple reaction monitoring.
abstract: A novel method for the determination of cis-thymine glycol in DNA has been developed, using gas chromatography-mass spectrometry with selected ion recording or multiple reaction monitoring. The procedure involves acidic hydrolysis of DNA in the presence of the internal standard cis-[2H3]thymine glycol, followed by derivatisation with N-methyl-N-(tert.-butyldimethylsilyl)trifluoroacetamide. The method was validated on DNA that had been oxidatively modified in vitro by radiation treatment, and was then applied to determine cis-thymine glycol in human placental DNA. Background levels of 5.45+/-2.98 ng cis-thymine glycol/mg DNA were observed in the human samples.
authors.affiliation: M.R.C. Toxicology Unit, University of Leicester, UK.
authors.forename: S
authors.initials: S
authors.lastname: Farooq
authors.forename: E
authors.initials: E
authors.lastname: Bailey
authors.forename: P B
authors.initials: PB
authors.lastname: Farmer
authors.forename: R
authors.initials: R
authors.lastname: Jukes
authors.forename: J H
authors.initials: JH
authors.lastname: Lamb
authors.forename: H
authors.initials: H
authors.lastname: Hernández
authors.forename: R
authors.initials: R
authors.lastname: Sram
authors.forename: J
authors.initials: J
authors.lastname: Topinka
mesh_terms: D000081:Acetamides; D000818:Animals; D000975:Antioxidants / Q000494:pharmacology; D002138:Calibration; D002417:Cattle; D004247:DNA / Q000737:chemistry*; D005260:Female; D005463:Fluoroacetates; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods*; D006801:Humans; D006868:Hydrolysis; D017646:Organosilicon Compounds / Q000737:chemistry; D009994:Osmolar Concentration; D010920:Placenta / Q000737:chemistry / Q000473:pathology; D011247:Pregnancy; D012015:Reference Standards; D013237:Stereoisomerism; D013941:Thymine / Q000031:analogs & derivatives* / Q000032:analysis / Q000737:chemistry; D013950:Thymus Gland / Q000737:chemistry; D014269:Trifluoroacetic Acid / Q000737:chemistry; D014316:Tritium
chemical_list: D000081:Acetamides; D000975:Antioxidants; D005463:Fluoroacetates; D017646:Organosilicon Compounds; D014316:Tritium; C029389:thymine glycol; C059151:N-methyl-N-(tert-butyldimethylsilyl)trifluoroacetamide; D004247:DNA; D014269:Trifluoroacetic Acid; D013941:Thymine