title: Damaged chromatin does not prevent the exit from metaphase I in fused mouse oocytes.
abstract: The presence of checkpoint mechanisms which are able to recognize damaged chromatin and thereafter to prevent exit from metaphase I has been investigated in giant mouse oocytes produced by fusion of a normal metaphase I oocyte with an equivalent oocyte with damaged chromatin. The presence of damaged chromatin did not prevent the onset of anaphase I in both sets of chromatin in the fused cells. Interestingly, fused or unfused cells containing only damaged chromatin failed to enter anaphase and persisted instead in a metaphase-like state. These results demonstrate the fragility of checkpoint controls in mammalian female germ cells.
authors.affiliation: Institute of Animal Production, Prague, Czech Republic.
authors.forename: J
authors.initials: J
authors.lastname: Fulka
authors.forename: P
authors.initials: P
authors.lastname: Kalab
authors.forename: N L
authors.initials: NL
authors.lastname: First
authors.forename: R M
authors.initials: RM
authors.lastname: Moor
mesh_terms: D000818:Animals; D002459:Cell Fusion; D002843:Chromatin / Q000473:pathology*; D005260:Female; D008677:Metaphase; D051379:Mice; D009865:Oocytes / Q000473:pathology*
chemical_list: D002843:Chromatin