Characterization of a t(8;13)(p11;q11-12) in an atypical myeloproliferative disorder.
Fourteen cases of an atypical myeloproliferative disorder associated with consistent translocations involving 8p11-12 have previously been described. A t(8;13)(p11;q11-12) was the most common, but variant t(8;9)(p11-12;q32-34) and t(6;8)(q27;p12) were also reported. Here we have used a series of yeast artificial chromosomes (YACs) derived from the 8p11 and 13q11-12 regions to analyse one of the t(8;13) cases by fluorescence in situ hybridization (FISH). YACs flanking the 13q11-12 breakpoint and spanning the 8p11 breakpoint have been isolated. These YACs will facilitate characterization of the genes involved in this rearrangement.
Smedley
D
D
Institute of Cancer Research, Sutton, Surrey, United Kingdom.
Somers
G
G
Venter
D
D
Chow
C W
CW
Cooper
C
C
Shipley
J
J
D018244:Chromosomes, Artificial, Yeast; D002882:Chromosomes, Human, Pair 13 / Q000235:genetics*; D002898:Chromosomes, Human, Pair 8 / Q000235:genetics*; D006801:Humans; D007621:Karyotyping; D008677:Metaphase; D009196:Myeloproliferative Disorders / Q000235:genetics*; D009693:Nucleic Acid Hybridization; D014178:Translocation, Genetic