Detection of Escherichia cole O157 in French food samples using an immunomagnetic separation method and the VIDAS E. coli O157.
Two commercially available screening methods, an automated enzyme-linked fluorescent immunoassay (VIDAS E. coli O157) and an immunomagnetic separation followed by culture onto cefixime tellurite sorbitol MacConkey agar (CT-SMAC), were compared for detection of Escherichia coli O157 in naturally and artificially contaminated food samples. A total of 250 naturally contaminated food samples, including raw milk cheeses, poultry, raw sausages and ground beef retail samples, were examined. Four poultry, one raw sausage and one ground beef sample were found to be positive for E. coli O157 by both methods. Of the six positive samples, five were shown to contain sorbitol-positive, O157-positive, H7-negative, motile and non-verotoxin-producing E. coli.
Vernozy-Rozand
C
C
Unité de Microbiologie Alimentaire et Microbiologie Prévisionelle, Ecole Nationale Vétérinaire de Lyon, Marcy L'Etoile, France.
Mazuy
C
C
Ray-Gueniot
S
S
Boutrand-Loeï
S
S
Meyrand
A
A
Richard
Y
Y
D002611:Cheese / Q000382:microbiology; D019453:Escherichia coli O157 / Q000302:isolation & purification*; D005516:Food Microbiology; D007124:Immunoenzyme Techniques; D018189:Immunomagnetic Separation; D008460:Meat / Q000382:microbiology