title: A difference in the pattern of repair in a large genomic region in UV-irradiated normal human and Cockayne syndrome cells.
abstract: Xeroderma pigmentosum group C cells repair DNA damaged by ultraviolet radiation in an unusual pattern throughout the genome. They remove cyclobutane pyrimidine dimers only from the DNA of transcriptionally active chromatin regions and only from the strand that contains the transcribed strand. The repair proceeds in a manner that creates damage-free islands which are in some cases much larger than the active gene associated with them. For example, the small transcriptionally active beta-actin gene (3.5 kb) is repaired as part of a 50 kb single-stranded region. The repair responsible for creating these islands requires active transcription, suggesting that the two activities are coupled. A preferential repair pathway in normal human cells promotes repair of actively transcribed DNA strands and is coupled to transcription. It is not known if similar large islands, referred to as repair domains, are preferentially created as a result of the coupling. Data are presented showing that in normal cells, preferential repair in the beta-actin region is associated with the creation of a large, completely repaired region in the partially repaired genome. Repair at other genomic locations which contain inactive genes (insulin, 754) does not create similar large regions as quickly. In contrast, repair in Cockayne syndrome cells, which are defective in the preferential repair pathway but not in genome-overall repair, proceeds in the beta-actin region by a mechanism which does not create preferentially a large repaired region. Thus a correlation between the activity required to preferentially repair active genes and that required to create repaired domains is detected. We propose an involvement of the transcription-repair coupling factor in a coordinated repair pathway for removing DNA damage from entire transcription units.
authors.affiliation: Department of Biological Sciences, Wright State University, Dayton, Ohio 45435, USA.
authors.forename: G A
authors.initials: GA
authors.lastname: Shanower
authors.forename: G J
authors.initials: GJ
authors.lastname: Kantor
mesh_terms: D000199:Actins / Q000235:genetics; D002460:Cell Line; D003057:Cockayne Syndrome / Q000235:genetics*; D004247:DNA / Q000032:analysis / Q000235:genetics; D004249:DNA Damage; D015342:DNA Probes; D004260:DNA Repair / Q000235:genetics*; D005347:Fibroblasts; D016678:Genome; D006801:Humans; D007328:Insulin / Q000235:genetics; D011740:Pyrimidine Dimers; D014466:Ultraviolet Rays; D014960:X Chromosome / Q000235:genetics; D014983:Xeroderma Pigmentosum / Q000235:genetics
chemical_list: D000199:Actins; D015342:DNA Probes; D007328:Insulin; D011740:Pyrimidine Dimers; D004247:DNA