Cloning and analysis of the human complement factor H gene promoter.
The 5' flanking region of human factor H was cloned using nested polymerase chain reaction (PCR) and the promoter finder method. A total of 1.2 kb has been sequenced and a number of putative regulatory elements identified including glucocorticoid response elements cAMP responsive element, HTF-1, and acute phase signal sequences. A 717 b.p. fragment was cloned into a CAT reporter vector and transfected into HeLa cells. A series of truncations from the 5' end of this fragment were also cloned into the CAT vector. Analysis of CAT activity of the cell lysates showed that the region from -699 to +18 is likely to contain promoter elements for the factor H gene as it was able to drive transcription of the CAT gene.
Ward
H M
HM
Department of Microbiology and Infectious Diseases, Flinders Medical Centre, Bedford Park, Australia. helena.ward@flinders.edu.au
Higgs
N H
NH
Blackmore
T K
TK
Sadlon
T A
TA
Gordon
D L
DL
D001483:Base Sequence; D003001:Cloning, Molecular; D017242:Complement Factor H / Q000235:genetics*; D018076:DNA, Complementary / Q000302:isolation & purification; D006801:Humans; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D011401:Promoter Regions, Genetic / Q000235:genetics*; D017422:Sequence Analysis, DNA
C493495:CFH protein, human; D018076:DNA, Complementary; D017242:Complement Factor H