title: Temporal changes in spinal cord expression of mRNA for substance P, dynorphin and enkephalin in a model of chronic pain.
abstract: We have used a partial sciatic nerve ligation model to examine the time course for changes in the expression of mRNA for three peptides related to pain transmission at spinal sites (dynorphin, enkephalin and substance P), during the development of allodynia. Enhanced expression of mRNA for dynorphin and substance P was observed in the dorsal horn on the same side as the partial nerve ligation. Increased expression of dynorphin mRNA was biphasic. The initial increases in expression of dynorphin mRNA occurred at 3 h, and a secondary peak was observed 1-3 days after surgery. The secondary increases coincided roughly with increased substance P mRNA expression. However, both dynorphin and substance P mRNA returned to control values after 1 week despite continuing allodynia. No significant changes in expression of mRNA for enkephalin were observed. The elevation of substance P mRNA in intrinsic spinal cord neurons may be secondary to changes in immediate early genes c-fos and jun-B, whereas the expression of dynorphin and enkephalin mRNA is differently regulated. The results also suggest that changes in the expression of the three neuropeptides are not critically involved in the development and maintenance of chronic pain or allodynia.
authors.affiliation: College of Pharmacy, Oregon State University, Corvallis 97331, USA.
authors.forename: G E
authors.initials: GE
authors.lastname: Delander
authors.forename: E
authors.initials: E
authors.lastname: Schött
authors.forename: E
authors.initials: E
authors.lastname: Brodin
authors.forename: B B
authors.initials: BB
authors.lastname: Fredholm
mesh_terms: D000818:Animals; D001522:Behavior, Animal / Q000502:physiology; D002908:Chronic Disease; D004195:Disease Models, Animal; D004399:Dynorphins / Q000235:genetics; D004745:Enkephalins / Q000235:genetics; D015870:Gene Expression; D008297:Male; D009479:Neuropeptides / Q000235:genetics*; D010146:Pain / Q000378:metabolism* / Q000503:physiopathology; D012333:RNA, Messenger / Q000032:analysis / Q000235:genetics / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley; D013116:Spinal Cord / Q000737:chemistry / Q000378:metabolism*; D013373:Substance P / Q000235:genetics; D013997:Time Factors
chemical_list: D004745:Enkephalins; D009479:Neuropeptides; D012333:RNA, Messenger; D013373:Substance P; D004399:Dynorphins