Adhesion molecule expression in vascular endothelial cells incubated with cancer cell line supernatant are inhibited by neutrophil elastase inhibitor (ONO-5046.Na).
We investigated the role of a neutrophil elastase (NE) produced by cancer cell lines on intercellular adhesion molecule-1 (ICAM-1) expression in endothelial cells using two human non-small cell lung cancer cell lines, EBC-1 and PC-3. It is known that immuno-reactive NE is produced by EBC-1 cells, but not PC-3. In control study, Northern blot analysis revealed that in vitro ICAM-1 mRNA transcripts in the rat endothelial cell line (WK-5) was increased in the presence of purified NE while a specific neutrophil elastase inhibitor (ONO-5046.Na) inhibited ICAM-1 expression. Similarly, ICAM-1 mRNA levels in WK-5 cells were increased by the culture supernatant obtained from EBC-1, but not from PC-3. In addition, ONO-5046.Na inhibited the increase in ICAM-1 mRNA levels stimulated by immuno-reactive HE from EBC-1. These results suggest that EBC-1 cells produce immunoreactive NE which has biological function of NE to stimulate ICAM-1 expression in the endothelial cells.
Matsumura
F
F
Department of Surgery II, Kumamoto University Medical School, Japan.
Yamaguchi
Y
Y
Ogawa
M
M
D000818:Animals; D002289:Carcinoma, Non-Small-Cell Lung / Q000473:pathology*; D002294:Carcinoma, Squamous Cell / Q000473:pathology*; D004730:Endothelium, Vascular / Q000378:metabolism*; D005998:Glycine / Q000031:analogs & derivatives* / Q000494:pharmacology; D006801:Humans; D018799:Intercellular Adhesion Molecule-1 / Q000096:biosynthesis* / Q000235:genetics; D019272:Leukocyte Elastase / Q000037:antagonists & inhibitors*; D008175:Lung Neoplasms / Q000473:pathology*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D051381:Rats; D015842:Serine Proteinase Inhibitors / Q000494:pharmacology*; D013449:Sulfonamides / Q000494:pharmacology*; D014158:Transcription, Genetic; D014407:Tumor Cells, Cultured
D012333:RNA, Messenger; D015842:Serine Proteinase Inhibitors; D013449:Sulfonamides; D018799:Intercellular Adhesion Molecule-1; C069195:sivelestat; D019272:Leukocyte Elastase; D005998:Glycine