Expression of beta 2 integrins on blood leukocytes of cows with or without bovine leukocyte adhesion deficiency.
Peripheral blood leukocytes of 11 normal cows, 7 cows heterozygous and 2 heifers homozygous for bovine leukocyte adhesion deficiency (BLAD) were analysed by flow cytometry for the intensity of their beta 2 integrin expression (LFA-1(CD11a/CD18), CR3 (CD11b/CD18) and CR4 (CD11c/CD18)). BLAD-homozygotes revealed no or a very weak expression of the beta 2 integrins and had a 10-fold and 4- to 5-fold increase in absolute number of neutrophils and monocytes, respectively, whereas the absolute number of lymphocytes remained normal. The mean fluorescence intensity (MFI) of the beta 2 integrins (CD18) in heterozygous animals was 56 to 90% of this in the normal cows (MFI between 14 and 512). The difference in the expression level was most pronounced for LFA-1 on the small cluster of lymphocytes with the highest MFI for LFA-1. Repeated analysis and phorbol myristate acetate stimulation revealed that the LFA-1 expression on this high-expressing cell population of the peripheral blood allowed a ready identification of BLAD-heterozygotes by flow cytometry.
Cox
E
E
Laboratory of Veterinary Immunology, Faculty of Veterinary Medicine, Universiteit Gent, Merelbeke, Belgium. eric.cox@rug.ac.be
Mast
J
J
MacHugh
N
N
Schwenger
B
B
Goddeeris
B M
BM
D000818:Animals; D018845:CD11 Antigens / Q000032:analysis; D018821:CD18 Antigens / Q000097:blood*; D002417:Cattle; D002418:Cattle Diseases / Q000097:blood* / Q000235:genetics; D006579:Heterozygote; D018370:Leukocyte-Adhesion Deficiency Syndrome / Q000097:blood / Q000235:genetics / Q000662:veterinary*; D007962:Leukocytes / Q000737:chemistry*; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology
D018845:CD11 Antigens; D018821:CD18 Antigens; D013755:Tetradecanoylphorbol Acetate