Detection of Helicobacter pylori in fecal samples of gnotobiotic mice infected with H. pylori by an immunomagnetic-bead separation technique.
By an immunomagnetic-bead (IMB) separation technique, isolation of Helicobacter pylori from gastrointestinal and fecal samples of gnotobiotic mice infected with the microorganism was tried. The isolation rate of H. pylori from stomach samples after IMB separation was not higher than that of direct culture of the samples. After IMB separation of feces, H. pylori was detectable by PCR, although H. pylori was not culturable.
Osaki
T
T
Division of Flow Cytometry, Kyorin University School of Medicine, Mitaka, Japan. osaki@kyorin-u.ac.jp
Taguchi
H
H
Yamaguchi
H
H
Kamiya
S
S
D000818:Animals; D005243:Feces / Q000382:microbiology*; D005260:Female; D005856:Germ-Free Life; D016480:Helicobacter pylori / Q000302:isolation & purification*; D018189:Immunomagnetic Separation; D051379:Mice; D011817:Rabbits