Overexpression of the jasmonate-inducible 23 kDa protein (JIP 23) from barley in transgenic tobacco leads to the repression of leaf proteins.
We investigated transgenic tobacco lines which express different amounts of the barley JIP 23. In these plants the amount of several proteins decreased proportionally to increasing amounts of JIP 23 whereas the transcript levels were constant as determined for the small and the large subunit of RuBPCase. However, the translation initiation of the rbcS transcript was found to be less efficient than in the wild type. In contrast, the jip 23 transcript was efficiently initiated, indicating that no unspecific impairment of initiation occurred. The data suggest that the barley JIP 23 leads to discrimination among certain tobacco transcripts during translation initiation.
Görschen
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Institut für Pflanzenbiochemie, Halle/Saale, Germany. egoersch@ipb.uni-halle.de
Dunaeva
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Reeh
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Wasternack
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D000085:Acetates / Q000494:pharmacology; D002245:Carbon Dioxide / Q000378:metabolism; D003517:Cyclopentanes / Q000494:pharmacology; D018076:DNA, Complementary / Q000235:genetics; D018744:DNA, Plant / Q000032:analysis / Q000235:genetics; D018506:Gene Expression Regulation, Plant / Q000235:genetics*; D001467:Hordeum / Q000737:chemistry*; D054883:Oxylipins; D010442:Peptide Chain Initiation, Translational; D010788:Photosynthesis; D018515:Plant Leaves / Q000378:metabolism; D010940:Plant Proteins / Q000032:analysis* / Q000235:genetics*; D030821:Plants, Genetically Modified; D010947:Plants, Toxic; D011132:Polyribosomes / Q000378:metabolism; D012333:RNA, Messenger / Q000096:biosynthesis; D018749:RNA, Plant / Q000096:biosynthesis; D012273:Ribulose-Bisphosphate Carboxylase / Q000032:analysis / Q000235:genetics; D014026:Nicotiana / Q000235:genetics / Q000378:metabolism; D019076:Transgenes / Q000235:genetics
D000085:Acetates; D003517:Cyclopentanes; D018076:DNA, Complementary; D018744:DNA, Plant; C109805:JIP23 protein, Hordeum vulgare; D054883:Oxylipins; D010940:Plant Proteins; D012333:RNA, Messenger; D018749:RNA, Plant; D002245:Carbon Dioxide; C072239:methyl jasmonate; C101625:RbcL protein, plastid; D012273:Ribulose-Bisphosphate Carboxylase