Annexins II and V inhibit cell migration.
Cell motility is a crucial component involved in wound healing, development, and tumor metastasis. This study investigated whether extracellular annexins, members of a calcium- and phospholipid-binding family of proteins, play a role in the migration of Lewis lung carcinoma cells. Using assays for wound closure and migration through 8-micron pores, it was found that annexins II and V significantly (> 40%) inhibited migration of these highly metastatic cells. Additionally, anti-annexin II antibodies enhanced migration of these same cells in the wound closure assay, while an irrelevant antibody (anti-calmodulin) showed no effect. These effects may be due to annexin-membrane binding and inhibition of phospholipid movement that is necessary for the formation of membrane protrusions.
Balch
C
C
Department of Cellular and Molecular Physiology, University of Cincinnati College of Medicine, Ohio 45236-0576, USA.
Dedman
J R
JR
D000818:Animals; D017306:Annexin A2 / Q000502:physiology*; D017304:Annexin A5 / Q000502:physiology*; D018827:Carcinoma, Lewis Lung; D002462:Cell Membrane / Q000502:physiology; D002463:Cell Membrane Permeability; D002464:Cell Migration Inhibition; D002465:Cell Movement; D005110:Extracellular Space / Q000502:physiology; D007158:Immunologic Techniques; D014407:Tumor Cells, Cultured; D014945:Wound Healing
D017306:Annexin A2; D017304:Annexin A5