Compartmentation of the type I regulatory subunit of cAMP-dependent protein kinase in cardiac ventricular muscle.
The species-dependent compartmentation of type I cAMP-dependent protein kinase (PKA I) and its dissociated regulatory subunit (RI) was examined in the heart by biochemical and immunohistochemical means. PKA I and RI were resolved from type II cAMP-dependent protein kinase and its regulatory subunit by DEAE-Sephacel chromatography of the supernatant and Triton X-100 soluble particulate fractions of heart homogenates. The relative amounts of holoenzymes and subunits were determined by cAMP-binding, protein kinase, 8-N3-[32P]cAMP photoaffinity labeling, and Western blot assays. Rat, rabbit, and guinea pig hearts all contained PKA I to varying degrees, but only in the supernatant fractions. Significant amounts of dissociated RI were found in the supernatant fractions, and to a lesser extent the particulate fractions, of these species. In contrast, though no PKA I was detected in the supernatant or particulate fractions of pig and beef heart, half of the cAMP-binding activity in the particulate fraction was attributed to RI. The results suggest that RI may associate with membrane fractions when it is not associated with the PKA catalytic subunit. Immunohistochemical studies of tissue sections from pig, beef, and rat cardiac ventricle using antibodies directed against RI also revealed species-dependent localization of RI. Cardiac myocyte intercalated discs were stained in pig and beef sections with additional sarcolemmal staining in beef sections. Rat ventricle, which contained large amounts of supernatant PKA I, showed nuclear staining. The localization of RI to cardiac myocyte intercalated discs and sarcolemma in certain species suggests a role(s) for this subunit in mediating cAMP-regulated events in these regions.
Reinitz
C A
CA
Department of Biochemistry and Molecular Biology, University of North Dakota School of Medicine, Grand Forks 58202-9037, USA.
Bianco
R A
RA
Shabb
J B
JB
D000818:Animals; D001345:Autoradiography; D001386:Azides / Q000378:metabolism; D015153:Blotting, Western; D002352:Carrier Proteins / Q000032:analysis* / Q000302:isolation & purification; D002417:Cattle; D002451:Cell Compartmentation; D002458:Cell Fractionation; D002852:Chromatography, Ion Exchange; D000242:Cyclic AMP / Q000031:analogs & derivatives / Q000378:metabolism; D054746:Cyclic AMP-Dependent Protein Kinase Type II; D017868:Cyclic AMP-Dependent Protein Kinases / Q000032:analysis* / Q000302:isolation & purification; D004591:Electrophoresis, Polyacrylamide Gel; D005455:Fluorescent Antibody Technique; D006168:Guinea Pigs; D006352:Heart Ventricles; D007150:Immunohistochemistry; D047908:Intracellular Signaling Peptides and Proteins; D009206:Myocardium / Q000166:cytology / Q000201:enzymology*; D019920:Photoaffinity Labels; D011485:Protein Binding; D011817:Rabbits; D051381:Rats; D017207:Rats, Sprague-Dawley; D012508:Sarcolemma / Q000201:enzymology; D013552:Swine
D001386:Azides; D002352:Carrier Proteins; D047908:Intracellular Signaling Peptides and Proteins; D019920:Photoaffinity Labels; C015482:protein kinase modulator; C017042:8-azidoadenosine-3',5'-monophosphate; D000242:Cyclic AMP; D054746:Cyclic AMP-Dependent Protein Kinase Type II; D017868:Cyclic AMP-Dependent Protein Kinases