Determination of cis-thymine glycol in DNA by gas chromatography-mass spectrometry with selected ion recording and multiple reaction monitoring.
A novel method for the determination of cis-thymine glycol in DNA has been developed, using gas chromatography-mass spectrometry with selected ion recording or multiple reaction monitoring. The procedure involves acidic hydrolysis of DNA in the presence of the internal standard cis-[2H3]thymine glycol, followed by derivatisation with N-methyl-N-(tert.-butyldimethylsilyl)trifluoroacetamide. The method was validated on DNA that had been oxidatively modified in vitro by radiation treatment, and was then applied to determine cis-thymine glycol in human placental DNA. Background levels of 5.45+/-2.98 ng cis-thymine glycol/mg DNA were observed in the human samples.
Farooq
S
S
M.R.C. Toxicology Unit, University of Leicester, UK.
Bailey
E
E
Farmer
P B
PB
Jukes
R
R
Lamb
J H
JH
Hernández
H
H
Sram
R
R
Topinka
J
J
D000081:Acetamides; D000818:Animals; D000975:Antioxidants / Q000494:pharmacology; D002138:Calibration; D002417:Cattle; D004247:DNA / Q000737:chemistry*; D005260:Female; D005463:Fluoroacetates; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods*; D006801:Humans; D006868:Hydrolysis; D017646:Organosilicon Compounds / Q000737:chemistry; D009994:Osmolar Concentration; D010920:Placenta / Q000737:chemistry / Q000473:pathology; D011247:Pregnancy; D012015:Reference Standards; D013237:Stereoisomerism; D013941:Thymine / Q000031:analogs & derivatives* / Q000032:analysis / Q000737:chemistry; D013950:Thymus Gland / Q000737:chemistry; D014269:Trifluoroacetic Acid / Q000737:chemistry; D014316:Tritium
D000081:Acetamides; D000975:Antioxidants; D005463:Fluoroacetates; D017646:Organosilicon Compounds; D014316:Tritium; C029389:thymine glycol; C059151:N-methyl-N-(tert-butyldimethylsilyl)trifluoroacetamide; D004247:DNA; D014269:Trifluoroacetic Acid; D013941:Thymine