title: Expression of glutamate receptor subunits 2/3 and 4 in the hypoglossal nucleus of the rat after neurectomy.
abstract: The immunoreactivity of glutamate receptor subunits 2/3 (GluR2/3) and 4 (GluR4) was studied following neurectomy of the hypoglossal nucleus (NH). After a short period of survival (at 1, 2, and 7 days postoperation, dpo), GluR2/3 immunoreactivity was barely dectectable in the operated side of HN. During these periods, GluR4 immunoreactivity was present, but was greatly reduced when compared with the GluR4 immunoreactivity in the unoperated side. The data suggest that of the 4 subunits of the AMPA receptor, GluR2/3 is the most susceptible receptor to the early stage of hypoglossal neurectomy, and GluR4 tolerated the lesion more than the others. It is also suggested that both GluR2/3 and 4 may play a very important neuroprotective role in the early stage of neuronal degeneration after axotomy, especially the former. Following a midterm survival period (14, 21, and 35 dpo), GluR2/3 immunoreactivity gradually reappeared in some neurons on the operated side of HN, which may indicate functional recovery. However, the number of GluR4-immunopositive neurons on the operated side of HN was greatly reduced. The reason for such a reduction is not known, but, from the speculative point of view, it is possible that the disappearance of GluR4-positive neurons may be related to their excitotoxic property, especially at 35 dpo, when neuronal cell death had already occurred. Following a long-term period of survival (i.e., 56, 90, and 120 dpo), the numbers of surviving neurons remained fairly constant, suggesting the possible cessation of neuronal death.
authors.affiliation: Department of Pharmacology, National University of Singapore.
authors.forename: F R
authors.initials: FR
authors.lastname: Tang
authors.forename: M K
authors.initials: MK
authors.lastname: Sim
mesh_terms: D000818:Animals; D003714:Denervation; D007002:Hypoglossal Nerve / Q000166:cytology / Q000378:metabolism*; D007150:Immunohistochemistry; D008297:Male; D009474:Neurons / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D018094:Receptors, Metabotropic Glutamate / Q000096:biosynthesis*
chemical_list: D018094:Receptors, Metabotropic Glutamate; C108822:metabotropic glutamate receptor 2; C102718:metabotropic glutamate receptor 3; C108823:metabotropic glutamate receptor 4