title: Stress-induced visceral hypersensitivity to rectal distension in rats: role of CRF and mast cells.
abstract: BACKGROUND
  Psychological factors have long been implicated in the aetiology of irritable bowel syndrome often associated with abdominal pain. This work was designed to study, in rats, the influence of partial restraint stress on the abdominal cramps induced by rectal distension and to determine the role of corticotropin releasing factor (CRF) and mast cells degranulation in this response.
  
  
  METHODS
  Abdominal contractions were electromyographically recorded. Thirty minutes after stress or intracerebroventricular CRF, rectal distension was performed by inflation of a balloon (0.4-1.2 mL). alpha-helical CRF9-41 or doxantrazole were administered centrally (15 min) and intraperitoneally (30 min), respectively, before stress. Histamine release and the number of mast cells were determined in colonic pieces from stressed and control rats.
  
  
  RESULTS
  Stress and CRF enhanced the number of abdominal cramps evoked by rectal distension without affecting rectal compliance. alpha-helical CRF9-41 and doxantrazole antagonized the stress and CRF-induced enhancement of abdominal cramps. Stress increased the colonic histamine content whereas the number of colonic mast cells was unchanged.
  
  
  CONCLUSIONS
  Stress enhances abdominal contractions in response to rectal distension in rats via pathways involving central CRF and intestinal mast cells.
authors.affiliation: Institut de Recherche Jouveinal, Fresnes, France.
authors.forename: M
authors.initials: M
authors.lastname: Gué
authors.forename: C
authors.initials: C
authors.lastname: Del Rio-Lacheze
authors.forename: H
authors.initials: H
authors.lastname: Eutamene
authors.forename: V
authors.initials: V
authors.lastname: Théodorou
authors.forename: J
authors.initials: J
authors.lastname: Fioramonti
authors.forename: L
authors.initials: L
authors.lastname: Buéno
mesh_terms: D000818:Animals; D016477:Artifacts; D002552:Cerebral Ventricles / Q000187:drug effects / Q000502:physiology* / Q000503:physiopathology; D003085:Colic; D003106:Colon / Q000187:drug effects / Q000502:physiology / Q000503:physiopathology*; D003346:Corticotropin-Releasing Hormone / Q000008:administration & dosage / Q000494:pharmacology*; D004576:Electromyography; D006636:Histamine Release; D006727:Hormone Antagonists / Q000008:administration & dosage / Q000494:pharmacology; D007274:Injections, Intraperitoneal; D007276:Injections, Intraventricular; D008297:Male; D008407:Mast Cells / Q000502:physiology*; D010446:Peptide Fragments / Q000008:administration & dosage / Q000494:pharmacology*; D051381:Rats; D017208:Rats, Wistar; D012007:Rectum / Q000187:drug effects / Q000502:physiology / Q000503:physiopathology*; D012149:Restraint, Physical; D013315:Stress, Psychological / Q000503:physiopathology*; D013892:Thioxanthenes / Q000008:administration & dosage / Q000494:pharmacology; D044004:Xanthones
chemical_list: D006727:Hormone Antagonists; D010446:Peptide Fragments; D013892:Thioxanthenes; D044004:Xanthones; D003346:Corticotropin-Releasing Hormone; C045089:corticotropin releasing hormone (9-41); C010134:doxantrazole