Overexpression of GML promotes radiation-induced cell cycle arrest and apoptosis.
Expression of the GML gene is regulated in a p53-dependent manner and is correlated with the sensitivity of esophageal cancer cells to anti-cancer drugs. To clarify the effect of GML expression on the sensitivity of cancer cells to ionizing radiation treatment, we established cell lines derived from p53-mutant human osteosarcoma HOS and esophageal carcinoma TE10 lines in which GML expression can be induced using the tetracycline-regulable system. Colony formation assay showed that the growth of cells expressing GML are inhibited in response to ionizing radiation, whereas cells not expressing GML were resistant to irradiation. Further investigation demonstrated that GML expression enhances G2/M arrest and apoptosis induced by gamma-irradiation. These results suggest that GML sensitizes cancer cells to ionizing radiation.
Kagawa
K
K
Department of Oncogene Research, Osaka University, Japan.
Inoue
T
T
Tokino
T
T
Nakamura
Y
Y
Akiyama
T
T
D017209:Apoptosis / Q000528:radiation effects*; D001859:Bone Neoplasms / Q000532:radiotherapy; D002277:Carcinoma / Q000532:radiotherapy; D002453:Cell Cycle / Q000528:radiation effects*; D018797:Cell Cycle Proteins; D004938:Esophageal Neoplasms / Q000532:radiotherapy; D058851:GPI-Linked Proteins; D005720:Gamma Rays; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D008565:Membrane Proteins / Q000096:biosynthesis* / Q000235:genetics; D009154:Mutation; D009363:Neoplasm Proteins; D009374:Neoplasms, Experimental / Q000532:radiotherapy*; D012516:Osteosarcoma / Q000532:radiotherapy; D011836:Radiation Tolerance; D014407:Tumor Cells, Cultured; D016159:Tumor Suppressor Protein p53 / Q000235:genetics*
D018797:Cell Cycle Proteins; C102519:GML protein, human; D058851:GPI-Linked Proteins; D008565:Membrane Proteins; D009363:Neoplasm Proteins; D016159:Tumor Suppressor Protein p53