title: Immunohistochemistry of collagen types II and X, and enzyme-histochemistry of alkaline phosphatase in the developing condylar cartilage of the fetal mouse mandible.
abstract: We investigated the immunohistochemical localisation of types II and X collagen as well as the cytochemical localisation of alkaline phosphatase in the developing condylar cartilage of the fetal mouse mandible on d 14-16 of pregnancy. On d 14 of pregnancy, although no immunostaining for types II and X collagen was observed, alkaline phosphatase activity was detected in all cells in the anlage of the future condylar process. On d 15 of pregnancy, immunostaining for both collagen types was simultaneously detected in the primarily formed condylar cartilage. Alkaline phosphatase activity was also detected in chondrocytes at this stage. By d 16 of pregnancy, the hypertrophic cell zone rapidly increased in size. These findings strongly support a periosteal origin for the condylar cartilage of the fetal mouse mandible, and show that progenitor cells for condylar cartilage rapidly or directly differentiate into hypertrophic chondrocytes.
authors.affiliation: 1st Department of Oral Anatomy, School of Dentistry, Tokyo Medical and Dental University, Japan. S.Shibata.oan1@dent.tmd.ac.jp
authors.forename: S
authors.initials: S
authors.lastname: Shibata
authors.forename: K
authors.initials: K
authors.lastname: Fukada
authors.forename: S
authors.initials: S
authors.lastname: Suzuki
authors.forename: Y
authors.initials: Y
authors.lastname: Yamashita
mesh_terms: D000469:Alkaline Phosphatase / Q000032:analysis*; D000818:Animals; D002356:Cartilage / Q000737:chemistry* / Q000196:embryology* / Q000201:enzymology; D003094:Collagen / Q000032:analysis*; D005865:Gestational Age; D006651:Histocytochemistry; D007150:Immunohistochemistry; D008335:Mandibular Condyle / Q000196:embryology* / Q000201:enzymology; D051379:Mice; D008813:Mice, Inbred ICR; D009024:Morphogenesis / Q000502:physiology; D010521:Periosteum / Q000502:physiology
chemical_list: D003094:Collagen; D000469:Alkaline Phosphatase