The human TRIDENT/HFH-11/FKHL16 gene: structure, localization, and promoter characterization.
We recently identified the winged-helix/fork head transcription factor Trident in mouse and described its expression in cycling cells. Here we report the isolation and characterization of the human TRIDENT (HGMW-approved symbol FKHL16) cDNA and gene. Homology between the human and the mouse Trident proteins was 79%. The gene consists of 10 exons and is located on chromosome 12 band p13. The winged-helix DNA-binding domain is encoded on three exons. Analysis of the promoter in synchronized Rat-1 fibroblasts revealed a fragment of 300 bases responsible for the cell cycle-specific expression of the TRIDENT gene.
Korver
W
W
Department of Immunology, University Hospital Utrecht, The Netherlands. w.korver@lab.azu.nl
Roose
J
J
Heinen
K
K
Weghuis
D O
DO
de Bruijn
D
D
van Kessel
A G
AG
Clevers
H
H
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002874:Chromosome Mapping; D002881:Chromosomes, Human, Pair 12; D018076:DNA, Complementary; D000072278:Forkhead Box Protein M1; D051858:Forkhead Transcription Factors; D006801:Humans; D051379:Mice; D008969:Molecular Sequence Data; D011401:Promoter Regions, Genetic; D017386:Sequence Homology, Amino Acid; D014157:Transcription Factors / Q000235:genetics*
D018076:DNA, Complementary; C107585:FOXM1 protein, human; D000072278:Forkhead Box Protein M1; D051858:Forkhead Transcription Factors; C491524:Foxm1 protein, mouse; C513169:Foxm1 protein, rat; D014157:Transcription Factors