title: Cytokine profiles for peripheral blood lymphocytes from patients with active pulmonary tuberculosis and healthy household contacts in response to the 30-kilodalton antigen of Mycobacterium tuberculosis.
abstract: Patients with active tuberculosis (TB) have a stronger humoral but a poorer cellular immune response to the secreted 30-kDa antigen (Ag) of Mycobacterium tuberculosis than do healthy household contacts (HHC), who presumably are more protected against disease. The basis for this observation was studied by examining the Th1 (interleukin 2 [IL-2] and gamma interferon [IFN-gamma])- and Th2 (IL-10 and IL-4)-type cytokines produced in response to the 30-kDa Ag by peripheral blood mononuclear cells (PBMC) from patients with active pulmonary TB (n = 7) and from HHC who were tuberculin (purified protein derivative) skin test positive (n = 12). Thirty-kilodalton-Ag-stimulated PBMC from TB patients produced significantly lower levels of IFN-gamma (none detectable) than did those from HHC (212 +/- 73 pg/ml, mean +/- standard error) (P < 0.001). Likewise, 30-kDa-Ag-stimulated PBMC from TB patients failed to express IFN-gamma mRNA by reverse transcription-PCR, whereas cells from HHC expressed the IFN-gamma gene. In contrast, 30-kDa-Ag-stimulated PBMC from TB patients produced significantly higher levels of IL-10 (403 +/- 80 pg/ml) than did those from HHC (187 +/- 66 pg/ml) (P < 0.013), although cells from both groups expressed the IL-10 gene. IL-2 and IL-4 were not consistently produced, and their genes were not expressed by 30-kDa-Ag-stimulated cells from either TB patients or HHC. After treatment with antituberculous drugs, lymphocytes from four of the seven TB patients proliferated and three of them expressed IFN-gamma mRNA in response to the 30-kDa Ag and produced decreased levels of IL-10.
authors.affiliation: Department of Microbiology, National Institute of Respiratory Diseases, Mexico D.F., Mexico.
authors.forename: M
authors.initials: M
authors.lastname: Torres
authors.forename: T
authors.initials: T
authors.lastname: Herrera
authors.forename: H
authors.initials: H
authors.lastname: Villareal
authors.forename: E A
authors.initials: EA
authors.lastname: Rich
authors.forename: E
authors.initials: E
authors.lastname: Sada
mesh_terms: D000942:Antigens, Bacterial / Q000276:immunology* / Q000302:isolation & purification; D000995:Antitubercular Agents / Q000627:therapeutic use; D002455:Cell Division; D016207:Cytokines / Q000235:genetics / Q000378:metabolism*; D006801:Humans; D007111:Immunity, Cellular; D007074:Immunoglobulin G / Q000032:analysis; D007371:Interferon-gamma / Q000235:genetics / Q000378:metabolism; D016753:Interleukin-10 / Q000235:genetics / Q000378:metabolism; D007376:Interleukin-2 / Q000235:genetics / Q000378:metabolism; D015847:Interleukin-4 / Q000235:genetics / Q000378:metabolism; D007963:Leukocytes, Mononuclear / Q000276:immunology / Q000378:metabolism; D008214:Lymphocytes / Q000166:cytology / Q000276:immunology* / Q000378:metabolism*; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000032:analysis / Q000378:metabolism; D012882:Skin Tests; D018417:Th1 Cells / Q000276:immunology / Q000378:metabolism; D018418:Th2 Cells / Q000276:immunology / Q000378:metabolism; D014373:Tuberculin / Q000276:immunology; D014397:Tuberculosis, Pulmonary / Q000097:blood / Q000188:drug therapy / Q000276:immunology*
chemical_list: D000942:Antigens, Bacterial; D000995:Antitubercular Agents; D016207:Cytokines; D007074:Immunoglobulin G; D007376:Interleukin-2; D012333:RNA, Messenger; D014373:Tuberculin; D016753:Interleukin-10; D015847:Interleukin-4; D007371:Interferon-gamma