title: Expression of a single gene produces both forms of skeletal muscle cyclic nucleotide-gated channels.
abstract: Cyclic nucleotide-gated cation channels in skeletal muscle are responsible for insulin-activated sodium entry into this tissue (J. E. M. McGeoch and G. Guidotti. J. Biol. Chem. 267: 832-841, 1992). These channels have previously been isolated from rabbit skeletal muscle by 8-bromoguanosine 3',5'-cyclic monophosphate (8-BrcGMP) affinity chromatography, which separates them into two populations differing in nucleotide affinity [L. C. Santy and G. Guidotti. Am. J. Physiol. 271 (Endocrinol. Metab. 34): E1051-E1060, 1996]. In this study, a polymerase chain reaction approach was used to identify skeletal muscle cyclic nucleotide-gated channel cDNAs. Rabbit skeletal muscle expresses the same cyclic nucleotide-gated channel as rabbit aorta (M. Biel, W. Altenhofen, R. Hullin, J. Ludwig, M. Freichel, V. Flockerzi, N. Dascal, U. B. Kaupp, and F. Hofmann. FEBS Lett. 329: 134-138, 1993). The entire cDNA for this gene was cloned from rabbit skeletal muscle and an antiserum to this protein produced. Expression of this cDNA produces a 63-kDa protein with cyclic nucleotide-gated channel activity. A similarly sized immunoreactive protein is present in sarcolemma. Purification of the expressed channels reveals that this single gene produces both native skeletal muscle channel populations.
authors.affiliation: Department of Molecular and Cellular Biology, Harvard University, Cambridge, Massachusetts 02138, USA.
authors.forename: L C
authors.initials: LC
authors.lastname: Santy
authors.forename: G
authors.initials: G
authors.lastname: Guidotti
mesh_terms: D000818:Animals; D001011:Aorta / Q000378:metabolism; D001483:Base Sequence; D002460:Cell Line; D002462:Cell Membrane / Q000502:physiology; D002846:Chromatography, Affinity; D006152:Cyclic GMP / Q000031:analogs & derivatives / Q000494:pharmacology*; D054815:Cyclic Nucleotide-Gated Cation Channels; D018076:DNA, Complementary; D006801:Humans; D007473:Ion Channels / Q000096:biosynthesis* / Q000302:isolation & purification / Q000502:physiology*; D008969:Molecular Sequence Data; D018482:Muscle, Skeletal / Q000378:metabolism*; D009131:Muscle, Smooth, Vascular / Q000378:metabolism; D009928:Organ Specificity; D011817:Rabbits; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000302:isolation & purification; D016415:Sequence Alignment; D012689:Sequence Homology, Nucleic Acid; D014162:Transfection
chemical_list: D054815:Cyclic Nucleotide-Gated Cation Channels; D018076:DNA, Complementary; D007473:Ion Channels; D011994:Recombinant Proteins; C016276:8-bromocyclic GMP; D006152:Cyclic GMP