Use of a quartz crystal microbalance to monitor immunoliposome--antigen interaction.
We have used quartz crystal microbalance (QCM)-based real-time biospecific interaction measurement to analyze the binding of immunoliposomes to antigen and examined the use of liposomes as signal-enhancing reagents in competitive QCM immunoassay. For the preparation of immunoliposomes, various amounts of bacterially produced lipid-tagged single-chain antibody against 2-phenyloxazolone were incorporated in phosphatidylcholine liposomes. The immunoliposomes bound specifically to immobilized hapten, and this binding was inhibited by soluble hapten in a concentration-dependent manner. In this competitive assay, antigen could be measured in the concentration range from 10(-5) to 10(-8) M.
Yun
K
K
Department of Bioengineering, Faculty of Bioscience and Biotechnology, Tokyo Institute of Technology, Yokohama, Japan.
Kobatake
E
E
Haruyama
T
T
Laukkanen
M L
ML
Keinänen
K
K
Aizawa
M
M
D000906:Antibodies / Q000008:administration & dosage* / Q000276:immunology; D000937:Antigen-Antibody Reactions; D000941:Antigens / Q000276:immunology*; D015374:Biosensing Techniques; D008081:Liposomes / Q000276:immunology*; D011791:Quartz; D014894:Weights and Measures
D000906:Antibodies; D000941:Antigens; D008081:Liposomes; D011791:Quartz