Suppression of infectious virus spread and corneal opacification by the combined use of recombinant interferon beta and interleukin-10 following corneal infection with herpes simplex virus-1 in mice.
The effects of Interleukin-10 (IL-10) and recombinant murine interferon-beta (rMuIFN-beta) on experimental corneal herpes simplex virus 1 (HSV-1) inoculation in BALB/c mice were examined. The mice were inoculated with the HSV-1 strain KOS at their corneas after abrasion. IL-10 was then administered topically once a day for 10 days beginning 2 days post inoculation, while rMuIFN-beta was administered once a day for 10 days beginning 1 day post inoculation. The local viral growth in the inoculated eyes and trigeminal ganglia was reduced in the rMuIFN-beta-treated mice but not in the IL-10-treated mice. In the mice treated with both rMuIFN-beta and IL-10, the degree of both the local viral growth and corneal opacification decreased. The establishment of HSV-1 latency in the trigeminal ganglia was partially prevented by rMuIFN-beta treatment but not by IL-10 treatment. The combined use of the cytokines resulted in both the suppression of viral spread and the prevention of corneal inflammation induced by HSV-1 infection.
Minagawa
H
H
Department of Virology, Faculty of Medicine, Kyushu University, Fukuoka, Japan. hmina@virology.med.kyushu-u.ac.jp
Sakai
Y
Y
Li
Y
Y
Ishibashi
T
T
Inomata
H
H
Mori
R
R
D000818:Animals; D003318:Corneal Opacity / Q000503:physiopathology / Q000628:therapy / Q000821:virology*; D004195:Disease Models, Animal; D004359:Drug Therapy, Combination; D018259:Herpesvirus 1, Human / Q000187:drug effects* / Q000254:growth & development; D006801:Humans; D016899:Interferon-beta / Q000494:pharmacology* / Q000627:therapeutic use; D016753:Interleukin-10 / Q000494:pharmacology* / Q000627:therapeutic use; D016849:Keratitis, Herpetic / Q000503:physiopathology / Q000628:therapy / Q000821:virology*; D051379:Mice; D008807:Mice, Inbred BALB C; D011994:Recombinant Proteins / Q000494:pharmacology / Q000627:therapeutic use; D010948:Viral Plaque Assay
D011994:Recombinant Proteins; D016753:Interleukin-10; D016899:Interferon-beta