Adenosine(5') oligophospho-(5') guanosines and guanosine(5') oligophospho-(5') guanosines in human platelets.
We isolated and identified nucleoside(5') oligophospho-(5') nucleosides containing adenosine and guanosine (ApnG; n = 3-6) as well as diguanosine polyphosphates (GpnG; n = 3-6) in human platelets. For identification, UV spectrometry, matrix-assisted laser desorption/ionization, postsource decay matrix-assisted laser desorption/ionization mass spectrometry, and enzymatic cleavage experiments were used. The adenosine(5') oligophospho-(5') guanosines act as vasoconstrictors and growth factors. The diguanosine polyphosphates are potent modulators of growth in vascular smooth muscle cells, but do not affect vascular tone.
Schlüter
H
H
Med. Klinik I, Univ.-Klinik Marienhospital, University of Bochum, Hölkeskampring 40, 44625 Herne, Germany.
Grobeta
I
I
Bachmann
J
J
Kaufmann
R
R
van der Giet
M
M
Tepel
M
M
Nofer
J R
JR
Assmann
G
G
Karas
M
M
Jankowski
J
J
Zidek
W
W
D000241:Adenosine; D001792:Blood Platelets / Q000378:metabolism*; D015226:Dinucleoside Phosphates / Q000302:isolation & purification / Q000378:metabolism*; D005110:Extracellular Space / Q000378:metabolism; D006151:Guanosine; D006801:Humans; D007668:Kidney / Q000378:metabolism; D009131:Muscle, Smooth, Vascular / Q000201:enzymology; D009708:Nucleotidases / Q000378:metabolism; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods; D014662:Vasoconstrictor Agents / Q000302:isolation & purification / Q000378:metabolism*
D015226:Dinucleoside Phosphates; D014662:Vasoconstrictor Agents; D006151:Guanosine; D009708:Nucleotidases; C030287:nucleotidase; D000241:Adenosine