A quantitative assessment of serum chylomicron by light scattering intensity: application to the intestinal fat absorption test.
A novel fat absorption test to clarify the malabsorption syndrome was developed using a micronephelometric technique and compared with the classic conventional technique using 131I-triolein. An integrity of time-sequential light scattered from chylomicron-related turbidity in serum was determined between 0 and 300 min after butter fat load, being expressed in terms of the light scattering intensity (LSI). A good correlation was obtained between LSI and the serum level of chylomicron-triglyceride determined by an ultracentrifugation technique (r = 0.819, P < 0.001). The maximal LSI was consistently observed at 180 min after administration of a test meal in the normal group (n = 39), whereas the malabsorption syndrome group (n = 35) was distinctly different and could be further classified according to four patterns of LSI changes. In addition, an inverse correlation was found between this fat absorption test and the 131I-triolein absorption test. It was concluded that the micronephelometric technique which does not use a radionuclide is advantageous in its simple and safe evaluation of fat malabsorption syndrome.
Tazuma
S
S
First Department of Internal Medicine, Hiroshima University School of Medicine, Japan.
Miura
H
H
Hirano
N
N
Hattori
Y
Y
Kajihara
T
T
Tsuchimoto
D
D
Miyake
H
H
Nishioka
T
T
Hyogo
H
H
Nakao
S
S
Yamashita
G
G
Kajiyama
G
G
D000328:Adult; D002079:Butter; D002623:Chemistry Techniques, Analytical / Q000295:instrumentation; D002914:Chylomicrons / Q000097:blood*; D004041:Dietary Fats / Q000378:metabolism; D005260:Female; D006801:Humans; D007457:Iodine Radioisotopes; D008027:Light; D008074:Lipoproteins / Q000097:blood / Q000648:ultrastructure; D008286:Malabsorption Syndromes / Q000378:metabolism / Q000503:physiopathology*; D008297:Male; D008854:Microscopy, Electron; D008875:Middle Aged; D014280:Triglycerides / Q000097:blood / Q000737:chemistry; D014304:Triolein
D002914:Chylomicrons; D004041:Dietary Fats; D007457:Iodine Radioisotopes; D008074:Lipoproteins; D014280:Triglycerides; D014304:Triolein; D002079:Butter