title: Electron Tomography of Single Ice-Embedded Macromolecules: Three-Dimensional Alignment and Classification
abstract: From 3-D reconstructions of automatically recorded tilt series of ice-embedded macromolecules, several hundred 3-D images of single particles can be extracted. Here we describe correlation-based techniques to align the particles with respect to translation and orientation in 3-D and the calculation of an averaged reconstruction after application of the correct weighting function to the particle projections. Multivariate statistical analysis and classification are applied to the set of three-dimensionally reconstructed particles to investigate interimage variations on the 3-D level. Copyright 1997 Academic Press. Copyright 1997 Academic Press
authors.affiliation: Max-Planck-Institut fur Biochemie, Am Klopferspitz 18a, Martinsried, 82152, Germany
authors.initials: J
authors.lastname: Walz
authors.initials: D
authors.lastname: Typke
authors.initials: M
authors.lastname: Nitsch
authors.initials: AJ
authors.lastname: Koster
authors.initials: R
authors.lastname: Hegerl
authors.initials: W
authors.lastname: Baumeister