Protein-protein interaction between the RVS161 and RVS167 gene products of Saccharomyces cerevisiae.
As previous studies indicated that the RVS161 and RVS167 gene products of Saccharomyces cerevisiae seem to be involved in the same cellular function, we considered the possibility of a complex between the proteins encoded by these two genes. Using the two hybrid system, we have shown that Rvs167p interacts with Rvs161p, through its N-terminal domain which contains predicted coiled-coil structures. Moreover, if a tagged Rvs 167p protein was immuno adsorbed under non-denaturing conditions, it brought along the Rvs161p protein. These results confirmed the hypothesis of an in vivo complex between the two proteins.
Navarro
P
P
Laboratoire de Biologie Cellulaire des Levures, IBGC-CNRS, Bordeaux, France.
Durrens
P
P
Aigle
M
M
D015153:Blotting, Western; D003598:Cytoskeletal Proteins; D019281:Dimerization; D005656:Fungal Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D017930:Genes, Reporter / Q000235:genetics; D008840:Microfilament Proteins; D010957:Plasmids / Q000235:genetics; D011233:Precipitin Tests; D011487:Protein Conformation; D017433:Protein Structure, Secondary; D012441:Saccharomyces cerevisiae / Q000235:genetics / Q000378:metabolism*; D029701:Saccharomyces cerevisiae Proteins; D001616:beta-Galactosidase / Q000235:genetics / Q000378:metabolism
D003598:Cytoskeletal Proteins; D005656:Fungal Proteins; D008840:Microfilament Proteins; C089181:RVS161 protein, S cerevisiae; C082282:RVS167 protein, S cerevisiae; D029701:Saccharomyces cerevisiae Proteins; D001616:beta-Galactosidase