Monoamine metabolite levels in CSF of SIV-infected rhesus monkeys (Macaca mulatta).
Increasing evidence suggests the involvement of the basal ganglia in HIV-1-infected patients. We used SIV-macaques, an animal model of HIV-1 infection, to investigate changes in CSF biogenic amine metabolites over time and compared them with control animals. The dopamine and serotonin metabolites, 3,4-dihydroxyphenylacetic acid (DOPAC), homovanillic acid (HVA) and 5-hydroxyindoleacetic acid (5-HIAA), were analysed by reverse-phase chromatography with electrochemical detection. DOPAC concentrations were significantly increased in SIV-infected animals compared with controls. 5-HIAA and HVA remained unchanged. However, the longitudinal assessment of metabolites up to 3 months post-infection revealed a significant increase in 5-HIAA. Our results reflect the effects of SIV early stage infection on monoamine systems in brain and further validate the simian model for AIDS research.
Koutsilieri
E
E
Department of Psychiatry, Clinical Neurochemistry, Julius-Maximilians-University, Würzburg, Germany.
Götz
M E
ME
Sopper
S
S
Stahl-Hennig
C
C
Czub
M
M
ter Meulen
V
V
Riederer
P
P
D015102:3,4-Dihydroxyphenylacetic Acid / Q000134:cerebrospinal fluid; D000163:Acquired Immunodeficiency Syndrome / Q000134:cerebrospinal fluid*; D000818:Animals; D001679:Biogenic Amines / Q000134:cerebrospinal fluid*; D004298:Dopamine / Q000134:cerebrospinal fluid; D004563:Electrochemistry / Q000379:methods; D015497:HIV-1; D006719:Homovanillic Acid / Q000134:cerebrospinal fluid; D006801:Humans; D006897:Hydroxyindoleacetic Acid / Q000134:cerebrospinal fluid; D008253:Macaca mulatta; D012016:Reference Values; D012701:Serotonin / Q000134:cerebrospinal fluid; D015302:Simian Immunodeficiency Virus; D013997:Time Factors
D001679:Biogenic Amines; D015102:3,4-Dihydroxyphenylacetic Acid; D012701:Serotonin; D006897:Hydroxyindoleacetic Acid; D004298:Dopamine; D006719:Homovanillic Acid