Evaluation of DNA repair inhibition by antitumor or antibiotic drugs using a chemiluminescence microplate assay.
A recently derived in vitro chemiluminescence assay (Salles et al. [1995] Anal. Biochem., 232, 37-42) has been used to investigate the effects of a panel of twenty-two anticancer drugs and certain antibiotics on the excision repair activity of cell-free extracts from the human cell line, HeLa. This methodology, termed the 3D (Damaged DNA Detection) assay, based on the in vitro excision repair assay previously developed (Wood et al. [1988] Cell, 53, 97-106) has provided data indicating definite in vitro inhibition of DNA repair by actinomycin D, aphidicolin, doxorubicin, distamycin A and mithramycin A. This assay therefore offers the potential for identifying agents with the ability to inhibit DNA repair.
Barret
J M
JM
Département de Cancérologie Expérimentale, Institut de Recherche Pierre Fabre, Castres, France.
Salles
B
B
Provot
C
C
Hill
B T
BT
D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D000970:Antineoplastic Agents / Q000494:pharmacology*; D004260:DNA Repair / Q000187:drug effects*; D004269:DNA, Bacterial / Q000187:drug effects / Q000528:radiation effects; D004305:Dose-Response Relationship, Drug; D006367:HeLa Cells; D006801:Humans; D008163:Luminescent Measurements; D008722:Methods; D010957:Plasmids; D014466:Ultraviolet Rays
D000900:Anti-Bacterial Agents; D000970:Antineoplastic Agents; D004269:DNA, Bacterial