PDGF- and TGF-beta-induced changes in cell shape of invertebrate immunocytes: effect of calcium entry blockers.
The cellular activity of hemocytes from the marine mollusc Mytilus galloprovincialis was studied using computer-assisted microscopic image analysis. PDGF-AB and TGF-beta 1 caused changes in cellular shape and induced the immunocytes to migrate in a chemotactic manner. The effect of PDGF-AB was more potent than that of TGF-beta 1, and the responses were dose-correlated for PDGF-AB, while they were dose-dependent up to 5 pg/ml for TGF-beta 1. Moreover, the PDGF-AB response was extracellular Ca(2+)-independent, while TGF-beta 1 was Ca(2+)-dependent.
Ottaviani
E
E
Department of Animal Biology, University of Modena, Italy.
Sassi
D
D
Kletsas
D
D
D000818:Animals; D002118:Calcium / Q000378:metabolism*; D002121:Calcium Channel Blockers / Q000494:pharmacology; D002465:Cell Movement; D048429:Cell Size / Q000187:drug effects; D006434:Hemocytes / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D006801:Humans; D008974:Mollusca / Q000166:cytology*; D010455:Peptides / Q000494:pharmacology; D010982:Platelet-Derived Growth Factor / Q000494:pharmacology*; D016212:Transforming Growth Factor beta / Q000494:pharmacology*; D014700:Verapamil / Q000494:pharmacology; D020866:omega-Conotoxin GVIA
D002121:Calcium Channel Blockers; D010455:Peptides; D010982:Platelet-Derived Growth Factor; D016212:Transforming Growth Factor beta; C095477:platelet-derived growth factor AB; D020866:omega-Conotoxin GVIA; D014700:Verapamil; D002118:Calcium