title: Helicobacter pylori adhesin binding fucosylated histo-blood group antigens revealed by retagging.
abstract: The bacterium Helicobacter pylori is the causative agent for peptic ulcer disease. Bacterial adherence to the human gastric epithelial lining is mediated by the fucosylated Lewis b (Leb) histo-blood group antigen. The Leb-binding adhesin, BabA, was purified by receptor activity-directed affinity tagging. The bacterial Leb-binding phenotype was associated with the presence of the cag pathogenicity island among clinical isolates of H. pylori. A vaccine strategy based on the BabA adhesin might serve as a means to target the virulent type I strains of H. pylori.
authors.affiliation: Department of Microbiology, Umeâ University, SE-901 87 Umeâ, Sweden.
authors.forename: D
authors.initials: D
authors.lastname: Ilver
authors.forename: A
authors.initials: A
authors.lastname: Arnqvist
authors.forename: J
authors.initials: J
authors.lastname: Ogren
authors.forename: I M
authors.initials: IM
authors.lastname: Frick
authors.forename: D
authors.initials: D
authors.lastname: Kersulyte
authors.forename: E T
authors.initials: ET
authors.lastname: Incecik
authors.forename: D E
authors.initials: DE
authors.lastname: Berg
authors.forename: A
authors.initials: A
authors.lastname: Covacci
authors.forename: L
authors.initials: L
authors.lastname: Engstrand
authors.forename: T
authors.initials: T
authors.lastname: Borén
mesh_terms: D018829:Adhesins, Bacterial / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism; D000595:Amino Acid Sequence; D000942:Antigens, Bacterial; D001422:Bacterial Adhesion; D001426:Bacterial Proteins / Q000235:genetics / Q000502:physiology; D001482:Base Composition; D001483:Base Sequence; D019921:Biotinylation; D002462:Cell Membrane / Q000737:chemistry; D003001:Cloning, Molecular; D018387:Codon, Initiator; D005643:Fucose; D005753:Gastric Mucosa / Q000382:microbiology; D005798:Genes, Bacterial; D006001:Glycoconjugates / Q000378:metabolism; D016480:Helicobacter pylori / Q000302:isolation & purification / Q000378:metabolism* / Q000472:pathogenicity; D006801:Humans; D007983:Lewis Blood Group Antigens / Q000378:metabolism*; D008024:Ligands; D008969:Molecular Sequence Data; D014774:Virulence
chemical_list: D018829:Adhesins, Bacterial; D000942:Antigens, Bacterial; D001426:Bacterial Proteins; D018387:Codon, Initiator; D006001:Glycoconjugates; D007983:Lewis Blood Group Antigens; D008024:Ligands; C080866:cagA protein, Helicobacter pylori; D005643:Fucose