Experimental studies with perfluoro-octane for hemostasis during vitreoretinal surgery.
OBJECTIVE
To investigate the effect of perfluoro-octane on coagulation studies and on intraoperative hemostasis during vitreoretinal surgery in an animal model.


METHODS
In vitro study--comparison of coagulation profiles (bleeding time, whole blood clotting time, partial thromboplastin time, and one-stage prothrombin time) of blood taken from healthy volunteers with and without the addition of perfluoro-octane. In vivo study--comparison of times taken to achieve hemostasis in a rabbit model with large retinal arterial bleeding in vitrectomized and aphakic eyes with and without intraocular injection of perfluoro-octane.


RESULTS
In vitro study--perfluoro-octane had no significant effect on coagulation profiles. In vivo study--intraocular perfluoro-octane significantly reduced the time to achieve hemostasis (P < 0.01) at all infusion bottle heights in vitrectomized and aphakic rabbit eyes.


CONCLUSIONS
Perfluoro-octane may be used to control bleeding during vitreoretinal surgery. A direct effect on the clotting cascade could not be demonstrated.
Moreira Júnior
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CA
Department of Ophthalmology, Universidade Federal do Parana, Curitiba, Brazil.
Uscocovich
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CE
Moreira
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AT
D000328:Adult; D000818:Animals; D001777:Blood Coagulation / Q000187:drug effects* / Q000502:physiology; D004195:Disease Models, Animal; D004621:Embolization, Therapeutic / Q000379:methods*; D005123:Eye; D005466:Fluorocarbons / Q000008:administration & dosage* / Q000494:pharmacology; D006801:Humans; D007267:Injections; D007431:Intraoperative Complications / Q000209:etiology / Q000503:physiopathology / Q000628:therapy*; D011817:Rabbits; D012016:Reference Values; D012161:Retinal Artery / Q000187:drug effects / Q000503:physiopathology; D012166:Retinal Hemorrhage / Q000209:etiology / Q000503:physiopathology / Q000628:therapy*; D016896:Treatment Outcome; D014821:Vitrectomy / Q000009:adverse effects*
D005466:Fluorocarbons; C073608:perfluorooctane