Central action of insect neuropeptide, periplanetin CC-1, in mice.
The central action of periplanetin CC-1 (Pea-HrTH) (Glp-Val-Asn-Phe-Ser-Pro-Asn-TrpNH2), octapeptide of the insect adipokinetic hormone family (AKH-family), isolated from American cockroach-Periplaneta americana, was studied in Albino Swiss mice (20-25 g). CC-1 was injected intracerebroventricularly (i.c.v.) in the volume of 5 microliters at the dose of 50 ng/mouse. It was found that CC-1 showed strong analgesic activity in "writhing syndrome" test and in "hot plate" test. In addition, periplanetin CC-1 decreased the threshold for tonic seizures and increased mortality in pentetrazole-induced seizures, having no influence on the electric convulsions. CC-1 is known to be a glucagon-like AKH in insects. However, administered i.c.v. to mice, it did not increase the peripheral blood glucose level. The obtained results indicate that periplanetin CC-1 shows a biological action in vertebrates, but its metabolic effects are different than in insects.
Sieklucka-Dziuba
M
M
Department of Pharmacology, Medical University School, Lublin, Poland.
Morawska
D
D
Lombarska-Sliwińska
D
D
Konopińska
D
D
Kleinrok
Z
Z
D000818:Animals; D000927:Anticonvulsants / Q000494:pharmacology; D001522:Behavior, Animal / Q000187:drug effects*; D001786:Blood Glucose / Q000378:metabolism; D001923:Brain Chemistry / Q000187:drug effects; D004597:Electroshock; D005106:Exploratory Behavior / Q000187:drug effects; D005260:Female; D006591:Hexobarbital / Q000494:pharmacology; D006993:Hypnotics and Sedatives / Q000494:pharmacology; D007276:Injections, Intraventricular; D007301:Insect Hormones / Q000494:pharmacology*; D008297:Male; D051379:Mice; D009043:Motor Activity / Q000187:drug effects; D009479:Neuropeptides / Q000494:pharmacology*; D018377:Neurotransmitter Agents / Q000378:metabolism; D009842:Oligopeptides / Q000494:pharmacology*; D010147:Pain Measurement / Q000187:drug effects; D010433:Pentylenetetrazole; D004856:Postural Balance / Q000187:drug effects; D012890:Sleep / Q000187:drug effects
D000927:Anticonvulsants; D001786:Blood Glucose; D006993:Hypnotics and Sedatives; D007301:Insect Hormones; D009479:Neuropeptides; D018377:Neurotransmitter Agents; D009842:Oligopeptides; C110318:periplanetin CC-1; D006591:Hexobarbital; D010433:Pentylenetetrazole