Orphanin FQ is the major OFQ1-17-containing peptide produced in the rodent and monkey hypothalamus.
In order to investigate the processing of OFQ containing peptides in the hypothalamus we have developed a sensitive and quantitative radioimmunoassay for OFQ. We fractionated rodent and monkey hypothalamic extracts by reversed-phase high performance liquid chromatography and found that the extracts contained multiple peaks of OFQ immunoreactivity with the major peak co-eluting with synthetic OFQ1-17. Mouse hypothalamic extracts were also fractionated by SDS-PAGE to determine the apparent molecular weights of molecules containing the OFQ peptide. Multiple peaks of OFQ immunoreactivity, ranging in size from approximately 1 to 30 kilodaltons, were detected by this method. These results suggest that OFQ1-17 is processed to smaller peptides in mouse and monkey hypothalamic neurons.
Quigley
D I
DI
Vollum Institute for Advanced Biomedical Research, Oregon Health Sciences University, Portland 97201, USA.
McDougall
J
J
Darland
T
T
Zhang
G
G
Ronnekliev
O
O
Grandy
D K
DK
Allen
R G
RG
D000595:Amino Acid Sequence; D000818:Animals; D002851:Chromatography, High Pressure Liquid; D004591:Electrophoresis, Polyacrylamide Gel; D005260:Female; D007031:Hypothalamus / Q000737:chemistry* / Q000166:cytology / Q000378:metabolism; D017403:In Situ Hybridization; D008253:Macaca mulatta; D008297:Male; D051379:Mice; D008969:Molecular Sequence Data; D008970:Molecular Weight; D018847:Opioid Peptides / Q000032:analysis* / Q000302:isolation & purification / Q000378:metabolism; D010446:Peptide Fragments / Q000032:analysis / Q000302:isolation & purification; D012333:RNA, Messenger / Q000032:analysis; D011863:Radioimmunoassay; D051381:Rats; D017208:Rats, Wistar; D000097629:Nociceptin
D018847:Opioid Peptides; D010446:Peptide Fragments; D012333:RNA, Messenger; D000097629:Nociceptin