Quantification of apoptotic cells using isolated glomeruli.
In order to detect apoptotic cells in the kidney, the terminal deoxynucleotidyl transferase-mediated dUTP-biotin nick end-labeling (TUNEL) method is used in tissue sections. But the number of apoptotic cells per glomerulus in several experimental models of nephritides is about 1 per single tissue section even at peak levels. In this study, we have reported that the TUNEL method and immunostaining of cell-specific markers to a whole isolated glomerulus in combination with laser scan microscopy are potentially useful methods for the analysis of cell turnover within glomeruli.
Omori
S
S
Department of Cell Biology, Niigata University School of Medicine, Japan.
Morioka
T
T
Wakasugi
M
M
Kawachi
H
H
Arakawa
M
M
Shimizu
F
F
Oite
T
T
D000818:Animals; D017209:Apoptosis; D002452:Cell Count; D053938:DNA Fragmentation; D004253:DNA Nucleotidylexotransferase; D003856:Deoxyuracil Nucleotides; D007678:Kidney Glomerulus / Q000166:cytology*; D017737:Macrophages, Peritoneal / Q000502:physiology; D051381:Rats; D017208:Rats, Wistar; D012079:Renal Circulation / Q000502:physiology
D003856:Deoxyuracil Nucleotides; D004253:DNA Nucleotidylexotransferase