DNA typing of recipient blood after massive transfusion.
BACKGROUND
Transfusion is known to alter the recipient's blood group, protein allotype, and alloenzyme phenotype by the introduction of donor material and the dilution of his or her own cells and proteins. There is little information about typing the DNA of blood after transfusion.


METHODS
Ten adult patients who had undergone massive transfusion with white cell-containing blood components were studied. Pretransfusion and posttransfusion blood specimen DNA types were compared by using Southern blot and polymerase chain reaction (PCR) analyses.


RESULTS
DNA types were identical in all 90 paired observations.


CONCLUSIONS
These preliminary findings, in a limited number of patients, suggest that certain DNA methods may provide reliable typing of adult recipients after massive transfusion.
Wenk
R E
RE
Baltimore Rh Typing Laboratory, Baltimore, MD, USA.
Chiafari
P A
PA
D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000483:Alleles; D001803:Blood Transfusion; D015139:Blotting, Southern; D004247:DNA / Q000097:blood* / Q000235:genetics; D005260:Female; D006683:HLA-DQ Antigens / Q000097:blood / Q000235:genetics; D006801:Humans; D008297:Male; D008875:Middle Aged; D018598:Minisatellite Repeats / Q000235:genetics; D010641:Phenotype; D016133:Polymerase Chain Reaction; D012091:Repetitive Sequences, Nucleic Acid
D006683:HLA-DQ Antigens; D004247:DNA