Functional expression of opioid receptor-like receptor and its endogenous specific agonist nociceptin/orphanin FQ during mouse embryogenesis.
Expression of opioid receptor-like receptor (ORL1) and its endogenous peptide agonist nociceptin/orphanin FQ (N/OFQ) during mouse embryogenesis have been investigated. Transcripts of ORL1 and N/OFQ were detected by RT-PCR in mouse brain of day 8 embryo (E8) and the expression continued afterwards. Northern blot analysis revealed abundant expression of ORL1 at postnatal day 1 (P1) and N/OFQ at E17 and P1 in the brain but none was detected in other embryonic tissues. The presence of functional ORL1 in mouse embryonic brain was also confirmed by specific binding of [3H] N/OFQ (kd = 1.3 +/- 0.5 nM and Bmax = 72 +/- 9 fmol/mg protein) as well as by N/OFQ-stimulated G protein activation.
Wu
Y L
YL
Shanghai Institute of Cell Biology, Chinese Academy of Sciences.
Fan
G H
GH
Zhao
J
J
Zhang
Y
Y
Zhou
T H
TH
Ma
L
L
Pei
G
G
D000818:Animals; D001921:Brain / Q000196:embryology / Q000378:metabolism; D005314:Embryonic and Fetal Development; D005260:Female; D019204:GTP-Binding Proteins / Q000378:metabolism; D051379:Mice; D008807:Mice, Inbred BALB C; D018847:Opioid Peptides / Q000096:biosynthesis*; D009928:Organ Specificity; D011957:Receptors, Opioid / Q000819:agonists* / Q000096:biosynthesis* / Q000502:physiology; D000094942:Nociceptin Receptor; D000097629:Nociceptin
D019204:GTP-Binding Proteins; D018847:Opioid Peptides; D011957:Receptors, Opioid; D000094942:Nociceptin Receptor; D000097629:Nociceptin; C000722287:Oprl1 protein, mouse