Cloning and structural analysis of the cDNA and the gene encoding the murine type 1 sigma receptor.
We have isolated a mouse cDNA which codes for a functional type 1 sigma receptor. The functional identity of the cDNA was established by expressing the cDNA in mammalian cells and measuring the cDNA-induced binding of haloperidol. Using this cDNA as the probe, we have isolated a murine genomic clone which contains the type 1 sigma receptor gene in its entirety. We have sequenced the gene completely, deduced the exon-intron organization and analyzed the promoter region sequence for transcription factor binding sites. The gene (approximately 7 kbp) is TATA-less but contains CCAATC and GC boxes immediately upstream of the transcription start site. The gene consists of 4 exons and 3 introns. The 5'-flanking region contains putative binding sites for AP-1, AP-2, GATA-1 and steroid receptors.
Seth
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P
Department of Biochemistry and Molecular Biology, Medical College of Georgia, Augusta 30912-2100, USA.
Leibach
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FH
Ganapathy
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V
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics; D005091:Exons; D015698:Genomic Library; D007438:Introns; D051379:Mice; D008969:Molecular Sequence Data; D011401:Promoter Regions, Genetic; D017480:Receptors, sigma / Q000235:genetics*; D015183:Restriction Mapping; D017422:Sequence Analysis, DNA; D000097605:Sigma-1 Receptor
D018076:DNA, Complementary; D017480:Receptors, sigma; D000097605:Sigma-1 Receptor