Wound and ethylene induction of the ACC oxidase melon gene CM-ACO1 occurs via two direct and independent transduction pathways.
The enzyme ACC oxidase catalyses the last step of ethylene biosynthesis in plants. Expression of the melon ACC oxidase gene, CM-ACO1, is rapidly induced (within 10 min) by ethylene treatment or upon wounding in leaves. The inhibitor of ethylene action, 1-methylcyclopropene (1-MCP), inhibited the accumulation of ethylene-induced CM-ACO1 mRNA transcripts, while wound-induced expression of the gene was not affected. The 5'-untranslated region of the CM-ACO1 gene was fused to the beta-glucuronidase (GUS) reporter gene and the corresponding transgenic tobacco plants were analysed. Two separate regions of the CM-ACO1 promoter activated GUS expression in response to ethylene treatment and wounding. These results suggest that induction of CM-ACO1 gene expression occurs via two separate signal transduction pathways in response to wounding and ethylene treatment.
Bouquin
T
T
Laboratoire Ethylène et Mécanismes Moléculaires de la Maturation des Fruits UA-INRA, Ecole Nationale Supérieure Agronomique de Toulouse, France.
Lasserre
E
E
Pradier
J
J
Pech
J C
JC
Balagué
C
C
D000594:Amino Acid Oxidoreductases / Q000096:biosynthesis / Q000235:genetics*; D000596:Amino Acids / Q000096:biosynthesis / Q000235:genetics*; D000598:Amino Acids, Cyclic; D019662:Cucurbitaceae / Q000201:enzymology / Q000235:genetics*; D004790:Enzyme Induction / Q000187:drug effects; D005030:Ethylenes / Q000494:pharmacology*; D015971:Gene Expression Regulation, Enzymologic / Q000187:drug effects; D018506:Gene Expression Regulation, Plant / Q000187:drug effects; D017343:Genes, Plant; D018515:Plant Leaves / Q000201:enzymology / Q000235:genetics; D015398:Signal Transduction / Q000187:drug effects / Q000235:genetics*
D000596:Amino Acids; D000598:Amino Acids, Cyclic; D005030:Ethylenes; C023863:1-aminocyclopropane-1-carboxylic acid; C036216:ethylene; D000594:Amino Acid Oxidoreductases; C059460:1-aminocyclopropane-1-carboxylic acid oxidase