title: Value of phenotyping methods as an initial screening of Pseudomonas aeruginosa in epidemiologic studies.
abstract: When studying the epidemiology of Pseudomonas aeruginosa, determination of the similarity of isolates is crucial. In the present study the distinctive capacity of four phenotyping methods (antibiotic susceptibility patterns, serotyping, phage-typing and outer membrane protein [OMP] profile analysis) was determined and compared to pulsed-field gel electrophoresis (PFGE) of enzyme restricted chromosomal DNA. In all, 91 isolates of P. aeruginosa were cultured from ten patients. Antibiotic susceptibility patterns were concordant for all isolates. Serotyping yielded five, phage-typing eight, OMP profile analysis nine and PFGE seven distinct types of P. aeruginosa. Compared to PFGE, the distinctive capacities were 89% (81/91) for serotyping, 87% (79/91) for phage-typing, and 90% (82/91) for OMP profile analysis. When serotyping results were different, PFGE types also were different (exclusiveness 100%). However, isolates with the same serotype may have various PFGE patterns. In contrast, isolates with similar PFGE patterns could have different phage-types or OMP types. For the study of isolates of P. aeruginosa, serotyping provides a good initial selection to reduce the number of isolates that need to be genotyped.
authors.affiliation: Dept. of Internal Medicine, University Hospital Maastricht, The Netherlands.
authors.forename: D
authors.initials: D
authors.lastname: Bergmans
authors.forename: M
authors.initials: M
authors.lastname: Bonten
authors.forename: F
authors.initials: F
authors.lastname: van Tiel
authors.forename: C
authors.initials: C
authors.lastname: Gaillard
authors.forename: N
authors.initials: N
authors.lastname: London
authors.forename: S
authors.initials: S
authors.lastname: van der Geest
authors.forename: P
authors.initials: P
authors.lastname: de Leeuw
authors.forename: E
authors.initials: E
authors.lastname: Stobberingh
mesh_terms: D001425:Bacterial Outer Membrane Proteins / Q000032:analysis; D004269:DNA, Bacterial / Q000032:analysis; D016521:Electrophoresis, Gel, Pulsed-Field; D006801:Humans; D008403:Mass Screening; D008826:Microbial Sensitivity Tests; D010641:Phenotype; D011550:Pseudomonas aeruginosa / Q000737:chemistry / Q000145:classification* / Q000187:drug effects / Q000235:genetics
chemical_list: D001425:Bacterial Outer Membrane Proteins; D004269:DNA, Bacterial