Detection of benzo[a]pyrene-DNA adducts in human placenta and umbilical cord blood.
Placenta constitutes a vital organ of exchange between mother and foetus. In addition to this favourable effect for foetal development, placenta indirectly may allow transfer of several maternal blood xenobiotics. Human placenta and umbilical cord blood are interesting models for investigating maternal environment and the metabolism, the bioactivation and the transfer of carcinogens such as polycyclic aromatic hydrocarbons. We used them to assess the effect of a woman's smoking on the foetus. Few studies cover this subject. In pregnant women who have continued to smoke, benzo[a]pyrene compound of cigarette smoke is metabolically activated to diol-epoxide derivative: benzo[a]pyrene-trans-7,8-dihydrodiol-9,10-epoxide, ultimate carcinogen (BPDE-I). This derivative is covalently fixed on DNA and gives BPDE-I-DNA adducts. By a competitive immunoassay technique, we determined BDPE-I-DNA adducts in 20 samples of placenta and umbilical cord blood from women who smoked (n = 15) and who did not (n = 10). Tobacco consumption was checked by urinary cotinine determination. In the group of smokers levels of adducts were found in 13 specimens of placenta (from 10 to 60 fmol/50 micrograms of DNA) and 12 umbilical cord blood (from 10 to 22.15 fmol/50 micrograms of DNA) samples. These results indicate that a mother's tobacco consumption is linked to the accumulation of BPDE-I-DNA adducts in the placenta, which are seen in smaller quantities in the umbilical cord blood, probably because of the metabolic capacity of the placenta and the transfer of B[a]P from the mother to the foetus.
Arnould
J P
JP
Laboratoire de Toxicologie, Faculté de Pharmacie, Amiens, France.
Verhoest
P
P
Bach
V
V
Libert
J P
JP
Belegaud
J
J
D000293:Adolescent; D000328:Adult; D001564:Benzo(a)pyrene / Q000032:analysis*; D003367:Cotinine / Q000652:urine; D003429:Cross Reactions; D018736:DNA Adducts / Q000032:analysis* / Q000097:blood; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D005312:Fetal Blood / Q000737:chemistry*; D006801:Humans; D007231:Infant, Newborn; D007962:Leukocytes / Q000737:chemistry / Q000378:metabolism; D010920:Placenta / Q000737:chemistry*; D011247:Pregnancy; D012907:Smoking / Q000378:metabolism
D018736:DNA Adducts; D001564:Benzo(a)pyrene; D003367:Cotinine