Quantitation of human immunodeficiency virus type 1 RNA in plasma by using blood dried on filter paper.
We compared whole blood dried on filter paper to the standard assay with frozen cell-free plasma for use in the quantitation of the human immunodeficiency virus RNA load in blood. RNA values from filter paper, corrected for the hematocrit, gave results comparable to those of the standard assay in terms of sensitivity and reproducibility.
Fiscus
S A
SA
Department of Microbiology and Immunology, University of North Carolina at Chapel Hill, 27599-7140, USA. sfiscus@email.unc.edu
Brambilla
D
D
Grosso
L
L
Schock
J
J
Cronin
M
M
D005374:Filtration; D015497:HIV-1 / Q000235:genetics*; D006801:Humans; D012367:RNA, Viral / Q000097:blood*; D015203:Reproducibility of Results
D012367:RNA, Viral