Expression of the Fanconi anemia group C gene in hematopoietic cells is not influenced by oxidative stress, cross-linking agents, radiation, heat, or mitotic inhibitory factors.
The Fanconi anemia group C gene (FAC) encodes a 63-kDa protein that plays a role in the growth and differentiation of hematopoietic progenitor cells and in cellular resistance to bifunctional cross-linking agents. The function of the gene product is unknown, as are the factors that govern expression of the gene itself. Seeking to associate a function of this protein with a general metabolic pathway, we attempted to identify factors that induce or repress expression of the gene encoding it. Using two plasmids from which mutant FAC mRNA molecules were transcribed in vitro to serve as competitor mRNAs in quantitative-competitive reverse transcriptase-polymerase chain reaction analysis and novel rabbit antisera raised to recombinant FAC proteins, we quantified gene expression in human hematopoietic cells. We determined that FAC is expressed constitutively in unstimulated normal peripheral blood mononuclear leukocytes, in Epstein-Barr virus (EBV)-transformed B lymphocytes, and in the factor-dependent human myeloid leukemic cell line MO7e at levels of approximately 2000, 200, and 200 FAC mRNA molecules/cell, respectively, and in CD34+ cells from normal human bone marrow at approximately 2000 FAC mRNA molecules/cell. Neither mRNA nor protein increased in any of the cells studied after exposure to mitomycin C, diepoxybutane, hydrogen peroxide, gamma radiation, heat, transforming growth factor-beta, or interferon-gamma. Using these sensitive methods, we confirmed that the FAC gene is constitutively expressed, even in the face of extracellular factors for which the gene product is a known effector of resistance. We conclude that the protective functions of the FAC gene product do not depend upon stressor-induced FAC gene expression.
Tower
P A
PA
Department of Medicine, Oregon Health Sciences University, Portland 97201-3098, USA.
Christianson
T A
TA
Peters
S T
ST
Ostroski
M L
ML
Hoatlin
M E
ME
Zigler
A J
AJ
Heinrich
M C
MC
Rathbun
R K
RK
Keeble
W
W
Faulkner
G R
GR
Bagby
G C
GC
D000818:Animals; D018797:Cell Cycle Proteins; D002478:Cells, Cultured; D003432:Cross-Linking Reagents / Q000494:pharmacology*; D004268:DNA-Binding Proteins; D052218:Fanconi Anemia Complementation Group C Protein; D051856:Fanconi Anemia Complementation Group Proteins; D005786:Gene Expression Regulation / Q000187:drug effects / Q000528:radiation effects; D018882:Heat Stress Disorders; D006410:Hematopoiesis / Q000235:genetics*; D006801:Humans; D008938:Mitosis / Q000187:drug effects; D009000:Monocytes / Q000187:drug effects / Q000378:metabolism* / Q000473:pathology; D009687:Nuclear Proteins; D018384:Oxidative Stress; D011506:Proteins / Q000235:genetics*; D011817:Rabbits; D014162:Transfection
D018797:Cell Cycle Proteins; D003432:Cross-Linking Reagents; D004268:DNA-Binding Proteins; C497651:FANCC protein, human; D052218:Fanconi Anemia Complementation Group C Protein; D051856:Fanconi Anemia Complementation Group Proteins; D009687:Nuclear Proteins; D011506:Proteins