Enantioselective analysis of albendazole sulfoxide in plasma using the chiral stationary phase.
We present a method for the enantioselective analysis of albendazole sulfoxide (ABZSO) in plasma for application in clinical pharmacokinetic studies. ABZSO enantiomers were separated on a 5-micron Chiralcel OB-H column (4.6 x 150 mm) using hexane:ethanol (93:7, v/v) as the mobile phase and fluorescence detection. ABZSO was extracted with chloroform:isopropanol (8:2, v/v) from 500-microliter aliquots of acidified plasma, with full drug recovery. The proposed method presented quantitation limits of 20 ng/ml for (-)ABZSO and 50 ng/ml for (+)ABZSO and was linear up to a concentration of 5,000 ng/ml of each enantiomer.
Paias
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FO
Faculdade de Filosofia, Ciências e Letras de Ribeirão Preto-USP, Brazil.
Lanchote
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VL
Takayanagui
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OM
Bonato
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PS
D000328:Adult; D015766:Albendazole / Q000031:analogs & derivatives* / Q000097:blood / Q000493:pharmacokinetics / Q000627:therapeutic use; D000871:Anthelmintics / Q000097:blood* / Q000493:pharmacokinetics / Q000627:therapeutic use; D002851:Chromatography, High Pressure Liquid; D003551:Cysticercosis / Q000188:drug therapy / Q000378:metabolism; D005260:Female; D006801:Humans; D007700:Kinetics; D013050:Spectrometry, Fluorescence; D013056:Spectrophotometry, Ultraviolet; D013237:Stereoisomerism
D000871:Anthelmintics; D015766:Albendazole; C027186:albendazole sulfoxide