Characterization of opiate binding sites on the goldfish (Carassius auratus L.) pronephric leukocytes.
The head kidney is the main lymphopoietic organ of teleost fish. It is the source of leukocytes inhabiting the peritoneal cavity during an experimental peritoneal inflammation (Gruca et al., Folia Biol.-Kraków, 1997, 44, 137-142). The number of elicited peritoneal leukocytes is significantly lower in the goldfish with concomitant morphine injection than in their counterparts injected with the irritant only. Morphine may act directly on the head kidney leukocytes, as they are equipped with the selective naloxone-binding sites (Chadzińska et al., Arch. Immunol. Ther. Exp., 1997, in press). Further characterization of these opioid receptors (by radioligand binding techniques) indicates that the goldfish head kidney leukocytes possess at least two different opiate-binding sites: the [3H]naloxone binding site with a KD = 87 +/- 2.1 nM and Bmax = 298 +/- 15 fmol/mg protein; and the second, the [3H]naltrindole binding site with a KD = 37 +/- 5.5 nM and Bmax = 1,172 +/- 220 fmol/mg protein. The competition experiments with delta- (naltrindole), kappa- (nor-binaltorphimine) and mu- (cyprodime, naltrexone) selective ligands suggest that the naloxone-binding site is similar to mu 3 receptors described by Stefano et al. (Proc. Nat. Acad. Sci. USA, 1993, 90 11099-11103). Low affinity binding of selective ligands excludes the presence of neuronal-type mu- and delta-opioid receptors on goldfish leukocytes.
Józefowski
S
S
Institute of Pharmacology, Polish Academy of Sciences, Kraków, Poland.
Płytycz
B
B
D000818:Animals; D001665:Binding Sites; D006054:Goldfish / Q000097:blood / Q000378:metabolism*; D007962:Leukocytes / Q000378:metabolism*; D009019:Morphinans / Q000378:metabolism; D009020:Morphine / Q000378:metabolism*; D009270:Naloxone / Q000378:metabolism; D009271:Naltrexone / Q000031:analogs & derivatives / Q000378:metabolism; D009292:Narcotic Antagonists / Q000378:metabolism; D017465:Receptors, Opioid, delta / Q000378:metabolism*; D017450:Receptors, Opioid, mu / Q000378:metabolism*
D009019:Morphinans; D009292:Narcotic Antagonists; D017465:Receptors, Opioid, delta; D017450:Receptors, Opioid, mu; C051843:binaltorphimine; C058450:cyprodime; D009270:Naloxone; D009271:Naltrexone; D009020:Morphine; C055382:naltrindole