Neuronal and neuroendocrine expression of lim3, a LIM class homeobox gene, is altered in mutant zebrafish with axial signaling defects.
LIM class homeobox genes code for a family of transcriptional regulators that encode important determinants of cell lineage and cell type specificity. The lim3 gene from the zebrafish, Danio rerio, is highly conserved in sequence and expression pattern compared to its homologs in other vertebrates. In this paper we report immunocytochemical analysis of Lim3 protein expression in the pituitary, pineal, hindbrain, and spinal cord of the embryo, revealing an asymmetrical, lateral and late program of pituitary development in zebrafish, distinct from the pattern in other vertebrates. We studied Lim3 expression in no tail, floating head, and cyclops mutant embryos, all of which have midline defects, with special reference to spinal cord differentiation where Lim3 marks mostly motoneurons. cyclops embryos showed essentially normal Lim3 expression in the hindbrain and spinal cord despite the absence of the floor plate, while no tail mutant embryos, which lack a differentiated notochord, displayed an excess of Lim3-expressing cells in a generally normal pattern. In contrast, Lim3-positive cells largely disappeared from the posterior spinal cord in floating head mutants, except in patches that correlated with remnants of apparent floor plate cells. These results support the view that either notochord or floor plate signaling can specify Lim3-positive motoneurons in the spinal cord.
Glasgow
E
E
Laboratory of Molecular Genetics, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, Maryland 20892, USA.
Karavanov
A A
AA
Dawid
I B
IB
D000015:Abnormalities, Multiple / Q000235:genetics; D000595:Amino Acid Sequence; D000818:Animals; D019070:Cell Lineage; D002490:Central Nervous System / Q000002:abnormalities / Q000196:embryology / Q000378:metabolism; D018507:Gene Expression Regulation, Developmental; D005801:Genes, Homeobox; D006257:Head / Q000196:embryology; D018398:Homeodomain Proteins / Q000096:biosynthesis* / Q000235:genetics; D060850:LIM-Homeodomain Proteins; D008969:Molecular Sequence Data; D009419:Nerve Tissue Proteins / Q000096:biosynthesis* / Q000235:genetics; D009474:Neurons / Q000378:metabolism*; D009490:Neurosecretory Systems / Q000378:metabolism*; D010902:Pituitary Gland / Q000196:embryology / Q000378:metabolism; D012333:RNA, Messenger / Q000096:biosynthesis; D012249:Rhombencephalon / Q000196:embryology / Q000378:metabolism; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid; D013116:Spinal Cord / Q000196:embryology / Q000378:metabolism; D013623:Tail / Q000196:embryology; D014157:Transcription Factors; D015027:Zebrafish / Q000196:embryology* / Q000235:genetics; D029961:Zebrafish Proteins
D018398:Homeodomain Proteins; D060850:LIM-Homeodomain Proteins; D009419:Nerve Tissue Proteins; D012333:RNA, Messenger; D014157:Transcription Factors; D029961:Zebrafish Proteins; C110148:lhx3 protein, zebrafish