title: Characterisation of an HCV NS3/NS4A proteinase fusion protein expressed in E.coli using synthetic peptide substrates.
authors.affiliation: Roche Discovery Welwyn, Welwyn Garden City, Herts, U.K.
authors.forename: T C
authors.initials: TC
authors.lastname: Wilkinson
authors.forename: P R
authors.initials: PR
authors.lastname: Bunyard
authors.forename: K
authors.initials: K
authors.lastname: Quirk
authors.forename: C S
authors.initials: CS
authors.lastname: Wilkinson
mesh_terms: D000595:Amino Acid Sequence; D003001:Cloning, Molecular; D004926:Escherichia coli; D016174:Hepacivirus / Q000201:enzymology*; D006801:Humans; D009842:Oligopeptides / Q000138:chemical synthesis / Q000737:chemistry / Q000378:metabolism; D020365:RNA Helicases; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis / Q000378:metabolism; D012697:Serine Endopeptidases / Q000096:biosynthesis; D013379:Substrate Specificity; D017361:Viral Nonstructural Proteins / Q000096:biosynthesis* / Q000378:metabolism*; D043583:Nucleoside-Triphosphatase; D053487:DEAD-box RNA Helicases; D000085242:Viral Proteases
chemical_list: D009842:Oligopeptides; D020365:RNA Helicases; D011993:Recombinant Fusion Proteins; D012697:Serine Endopeptidases; D017361:Viral Nonstructural Proteins; C060028:NS3 protein, flavivirus; D043583:Nucleoside-Triphosphatase; D053487:DEAD-box RNA Helicases; D000085242:Viral Proteases; C093542:NS4 protein, hepatitis C virus