title: Importance of the gonadotropin-releasing hormone (GnRH) surge for induction of the preovulatory luteinizing hormone surge of the ewe: dose-response relationship and excess of GnRH.
abstract: The preovulatory LH surge in the ewe is stimulated by a large sustained surge of GnRH. We have previously demonstrated that the duration of this GnRH signal exceeds that necessary to initiate and sustain the LH surge. The objective of the present study was to determine whether a similar excess exists for amplitude of the GnRH surge. Experiments were performed using an animal model in which GnRH secretion was blocked by progesterone, which in itself does not block the pituitary response to GnRH. To assess the amplitude of the GnRH surge needed to induce the LH surge, we introduced artificial GnRH surges of normal contour and duration but varying amplitudes. Twelve ewes were run through 3 successive artificial follicular phases (total of 36). Six of these artificial follicular phases were positive controls, in which progesterone was removed, the estradiol stimulus was provided, and vehicle was infused. In these control cycles, animals generated endogenous LH surges. In the remaining artificial follicular phases, progesterone was not withdrawn, the estradiol stimulus was provided, and either vehicle (negative control) or GnRH solutions of varying concentrations (experimental) were infused. The circulating GnRH concentrations achieved by infusion were monitored. No LH surges were observed in negative controls, whereas LH surges were induced in experimental cycles provided a sufficient dose of GnRH was infused. A highly significant dose-response relationship was observed between the amplitude of the GnRH surge and both the amplitude of the LH surge and the area under the curve describing the LH response, but no such relationship existed between the amplitude of the GnRH surge and the duration of the LH response. In numerous cases, LH surges similar to those in the positive control animals resulted from infusion of amounts of GnRH estimated to be considerably less than those delivered to the pituitary during the endogenously generated GnRH/LH surge. These findings indicate that, in the ewe, increased GnRH secretion drives the preovulatory LH surge in a dose-dependent fashion, and they provide evidence that the amplitude of the GnRH surge may exceed that needed to generate the LH surge.
authors.affiliation: Department of Physiology, University of Michigan, Ann Arbor 48109, USA.
authors.forename: J M
authors.initials: JM
authors.lastname: Bowen
authors.forename: G E
authors.initials: GE
authors.lastname: Dahl
authors.forename: N P
authors.initials: NP
authors.lastname: Evans
authors.forename: L A
authors.initials: LA
authors.lastname: Thrun
authors.forename: Y
authors.initials: Y
authors.lastname: Wang
authors.forename: M B
authors.initials: MB
authors.lastname: Brown
authors.forename: F J
authors.initials: FJ
authors.lastname: Karsch
mesh_terms: D000818:Animals; D004305:Dose-Response Relationship, Drug; D005260:Female; D005498:Follicular Phase / Q000097:blood*; D007987:Gonadotropin-Releasing Hormone / Q000097:blood* / Q000494:pharmacology; D007986:Luteinizing Hormone / Q000097:blood*; D012756:Sheep / Q000097:blood
chemical_list: D007987:Gonadotropin-Releasing Hormone; D007986:Luteinizing Hormone