title: Helicobacter pylori genotypes influence serum pepsinogen C levels.
abstract: BACKGROUND
  Infection from Helicobacter pylori significantly influences pepsinogen A (PGA) and C (PGC) levels in serum. Increased PGA and PGC serum levels are observed in H. pylori positive patients, while a significant decrease is observed after eradication. Little is known about the relative role of H. pylori cytotoxic strains in this phenomenon. The aim of our study was to assess the influence of cagA genotype on circulating levels of PGA and PGC.
  
  
  METHODS
  We studied 81 consecutive H. pylori positive patients, 64 H. pylori negative patients and 18 healthy controls. H. pylori was evaluated histologically in two antral and two body biopsies (Giemsa and/or Warthin Starry staining). Extracted DNA was then submitted for PCR amplification of both the urease A and cagA genes. A serum obtained from each patient before endoscopy was used for specific radioimmunoassay measurement of PGA and PGC.
  
  
  RESULTS
  The urease A gene was found in all H. pylori positive patients, the cagA gene was detected in 55 H. pylori positive patients and in none of the H. pylori negative patients. PGA and PGC levels were significantly higher in H. pylori positive than in H. pylori negative patients. A significant association was found between cagA and raised serum PGC levels in patients with antral gastritis but not in patients with peptic ulcer. Serum PGA levels were not affected by cagA.
  
  
  CONCLUSIONS
  Our results indicate that cagA positivity may influence the circulating PGC levels, probably because it causes a higher grade of mucosal inflammation.
authors.affiliation: Department of Laboratory Medicine, University Hospital of Padova, Italy.
authors.forename: M
authors.initials: M
authors.lastname: Plebani
authors.forename: D
authors.initials: D
authors.lastname: Basso
authors.forename: F
authors.initials: F
authors.lastname: Navaglia
authors.forename: F
authors.initials: F
authors.lastname: DiMario
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000907:Antibodies, Bacterial / Q000097:blood; D005260:Female; D005756:Gastritis / Q000097:blood / Q000382:microbiology; D005798:Genes, Bacterial / Q000235:genetics / Q000276:immunology; D005838:Genotype; D016481:Helicobacter Infections / Q000097:blood*; D016480:Helicobacter pylori / Q000235:genetics* / Q000276:immunology; D006801:Humans; D008297:Male; D008875:Middle Aged; D010435:Pepsinogens / Q000097:blood*; D010437:Peptic Ulcer / Q000097:blood / Q000382:microbiology; D014510:Urease / Q000235:genetics
chemical_list: D000907:Antibodies, Bacterial; D010435:Pepsinogens; D014510:Urease