Elucidation of the structure of the core region and the complete structure of the R-type lipopolysaccharide of Erwinia carotovora FERM P-7576.
An R-type lipopolysaccharide (LPS) from Erwinia carotovora strain FERM P-7576 was studied after strong alkaline degradation and mild acid hydrolysis. The resulting products were analyzed by fast-atom bombardment mass spectrometry, one- and two-dimensional 1H and 13C NMR spectroscopy, dephosphorylation and methylation analysis. The following structure was proposed for the core region of the LPS: [formula in text] where Hep is L-glycero-D-manno-heptose and Kdo is 3-deoxy-D-manno-octulosonic acid. Some LPS species lack the beta-D-Galp residue or the beta-D-Galp-(1-->7)-alpha-Hepp disaccharide. With the known structures of lipid A [Fukuoka, S., Kamishima, H., Nagawa, Y., Nakanishi, H., Ishikawa, K., Niwa, Y., Tamiya, E. & Karube, I. (1992) Arch. Microbiol. 157, 311-318] and the core moiety, the complete LPS structure was established and confirmed by matrix-assisted laser desorption/ionisation time-of-flight mass spectrometry of the native and O-deacylated LPS.
Fukuoka
S
S
Research Center Borstel, Center for Medicine and Biosciences, Germany.
Knirel
Y A
YA
Lindner
B
B
Moll
H
H
Seydel
U
U
Zähringer
U
U
D002236:Carbohydrate Conformation; D002240:Carbohydrate Sequence; D008070:Lipopolysaccharides / Q000737:chemistry*; D009682:Magnetic Resonance Spectroscopy; D008745:Methylation; D008969:Molecular Sequence Data; D015394:Molecular Structure; D009005:Monosaccharides / Q000032:analysis; D016973:Pectobacterium carotovorum / Q000737:chemistry*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
D008070:Lipopolysaccharides; D009005:Monosaccharides