title: Amperometric glutamate biosensor based on poly(o-phenylenediamine) film electrogenerated onto modified carbon paste electrodes.
abstract: A glutamate biosensor was developed by electropolymerizing o-phenylenediamine on a dehydrogenase-NAD+ modified carbon paste electrode. The amperometric response to glutamate was obtained by means of the electrocatalytic oxidation of the enzymatically produced NADH, at an applied potential close to 0 V (Ag/AgCl). After optimizing carbon paste composition, polymer thickness and operating variables, a linear amperometric response to glutamate was obtained within the concentration range 5.0 x 10(-6)-7.8 x 10(-5) M with a detection limit of 3.8 x 10(-6) M. The biosensor was applied to the determination of glutamate in chicken bouillon cubes. Good accuracy was found with respect to a reference enzymatic spectrophotometric method.
authors.affiliation: Departamento de Química Física y Analítica, Universidad de Oviedo, Asturias, Spain.
authors.forename: S L
authors.initials: SL
authors.lastname: Alvarez-Crespo
authors.forename: M J
authors.initials: MJ
authors.lastname: Lobo-Castañón
authors.forename: A J
authors.initials: AJ
authors.lastname: Miranda-Ordieres
authors.forename: P
authors.initials: P
authors.lastname: Tuñón-Blanco
mesh_terms: D015374:Biosensing Techniques; D002244:Carbon; D004566:Electrodes; D018698:Glutamic Acid / Q000032:analysis*; D010655:Phenylenediamines
chemical_list: D010655:Phenylenediamines; D018698:Glutamic Acid; D002244:Carbon