Are BCG effects against urinary bladder carcinoma cell line T24 correlated with apoptosis in vitro?
Because we hypothesized that cell death when bacillus Calmette-Guérin (BCG) was instillated was correlated with apoptosis, cultured urinary bladder carcinoma cells were treated with BCG and examined whether they showed cell death due to apoptosis. Although the proliferative activity of T24 cells was suppressed, we could not recognize apoptosis clearly. Cell cycle analysis indicated an increased number of apoptotic cells, but no DNA degeneration was seen when DNA electrophoresis was applied, and few apoptotic cells were detected by an in situ apoptosis detection kit. As a result, cell death of T24 cells when BCG was applied may have other mechanisms except apoptosis.
Sasaki
A
A
Department of Urology, Hirosaki University School of Medicine, Aomori, Japan.
Kudoh
S
S
Mori
K
K
Takahashi
N
N
Suzuki
T
T
D017209:Apoptosis; D002452:Cell Count; D002453:Cell Cycle; D002455:Cell Division; D004273:DNA, Neoplasm / Q000032:analysis; D004587:Electrophoresis, Agar Gel; D006801:Humans; D007150:Immunohistochemistry; D015850:Interleukin-6 / Q000378:metabolism; D009163:Mycobacterium bovis / Q000502:physiology*; D014407:Tumor Cells, Cultured; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism; D001749:Urinary Bladder Neoplasms / Q000378:metabolism / Q000382:microbiology / Q000473:pathology*
D004273:DNA, Neoplasm; D015850:Interleukin-6; D014409:Tumor Necrosis Factor-alpha