title: Analysis of the 5'-flanking regions of the MHC-linked Hsp70-2 and Hsp70-3 genes of the rat.
abstract: We have analyzed the genomic interval between the MHC-linked heat shock protein 70 (Hsp70) genes Hsp70-2 and Hsp70-3 in the rat. The distance between the transcription start sites of both genes which are organized in a head-to-head orientation is 604 bp. Unlike Hsp70-2 the 5'untranslated region of Hsp70-3 is interrupted by an intron of about 3.9 kb. The Hsp70-3 promoter lacks a TATA box and heat shock elements, but contains two CRE elements, one Sp1-GC and two PuF binding sites. Analysis of the Hsp70-2 promoter by CAT assays reveals positive and negative regulatory elements extending into the Hsp70-3 gene.
authors.affiliation: Division of Immunogenetics, University of Göttingen, Germany.
authors.forename: H
authors.initials: H
authors.lastname: Friedrich
authors.forename: L
authors.initials: L
authors.lastname: Walter
authors.forename: E
authors.initials: E
authors.lastname: Günther
mesh_terms: D000818:Animals; D001483:Base Sequence; D019556:COS Cells; D005796:Genes / Q000235:genetics; D018840:HSP70 Heat-Shock Proteins / Q000235:genetics*; D008285:Major Histocompatibility Complex / Q000235:genetics; D008297:Male; D008969:Molecular Sequence Data; D011401:Promoter Regions, Genetic / Q000235:genetics*; D051381:Rats; D011993:Recombinant Fusion Proteins; D017422:Sequence Analysis, DNA; D013737:Testis; D014157:Transcription Factors; D014158:Transcription, Genetic / Q000235:genetics
chemical_list: D018840:HSP70 Heat-Shock Proteins; C558795:Hspa1l protein, rat; D011993:Recombinant Fusion Proteins; D014157:Transcription Factors