Biochemical properties of hemagglutinins in the mollusk Pomacea flagellata.
Extracts of the Mollusk Pomacea flagellata were found to contain two agglutinins (PFA-I and PFA-II) which reacted with erythrocytes from human and several animal species. The hemagglutinating activity was stable between pH 4.0 and pH 10.0, and was independent of divalent cations. The active components were purified and found to be glycoproteins having Mr of 30,000. The isoelectric points were estimated to be 4.7 and 4.9 for PFA-I and PFA-II respectively. Hapten inhibition assays indicated that the agglutinin and its isoforms are specific for beta-galactosides as well as for galactose containing glycoproteins.
Arreguín-Espinosa
R
R
Instituto de Química, Universidad Nacional Autónoma de México, Ciudad Universitaria, Coyoacán, México, D.F. arrespin@servidor.unam.mx
Arreguín-Lozano
B
B
D000116:Acetylgalactosamine / Q000494:pharmacology; D000373:Agglutinins / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D000596:Amino Acids / Q000032:analysis; D000818:Animals; D002241:Carbohydrates / Q000494:pharmacology; D002846:Chromatography, Affinity; D004591:Electrophoresis, Polyacrylamide Gel; D004903:Erythrocyte Aggregation / Q000187:drug effects; D005690:Galactose / Q000494:pharmacology; D006385:Hemagglutination Inhibition Tests; D006388:Hemagglutinins / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D006736:Horses; D006801:Humans; D007785:Lactose / Q000494:pharmacology; D037102:Lectins; D051379:Mice; D008809:Mice, Inbred C3H; D008934:Mitogens / Q000494:pharmacology; D008974:Mollusca / Q000737:chemistry*; D011817:Rabbits; D051381:Rats; D012680:Sensitivity and Specificity; D012685:Sepharose; D012756:Sheep; D013154:Spleen / Q000187:drug effects; D013552:Swine
D000373:Agglutinins; D000596:Amino Acids; D002241:Carbohydrates; D006388:Hemagglutinins; D037102:Lectins; D008934:Mitogens; C079920:Phalera flavescens agglutinin; D012685:Sepharose; D007785:Lactose; D000116:Acetylgalactosamine; D005690:Galactose