Molecular characterization of a new human minisatellite that is able to form single-stranded loops in vitro and is recognized by nuclear proteins.
We report the isolation of a new low polymorphic GC-rich human minisatellite locus (MsH42) that contains different recombination motifs and is homologous to sequences involved in immunoglobulin class-switching. Furthermore, we show that MsH42 undergoes slipped-strand mispairing during PCR indicating its ability to generate single-stranded loops. Specific DNA-protein complexes were detected in band-shifting experiments using nuclear extracts from mouse testes and human NC-37 cells. The possible implications of this minisatellite in recombination events is discussed.
Boán
F
F
Departamento de Bioquímica y Biología Molecular, Facultad de Biología, Universidad de Santiago, Galicia, Spain.
González
A I
AI
Rodríguez
J M
JM
Gómez-Márquez
J
J
D000818:Animals; D001483:Base Sequence; D004247:DNA / Q000235:genetics*; D004276:DNA, Satellite / Q000235:genetics* / Q000378:metabolism; D015894:Genome, Human; D006801:Humans; D051379:Mice; D008969:Molecular Sequence Data; D009687:Nuclear Proteins / Q000235:genetics / Q000378:metabolism; D011995:Recombination, Genetic; D017422:Sequence Analysis, DNA
D004276:DNA, Satellite; D009687:Nuclear Proteins; D004247:DNA