Expression of Na(+)-H+ exchanger isoforms NHE1 and NHE3 in kidney and blood cells of rabbit and rat.
Increased peripheral blood cell Na-H exchange (NHE) and erythrocyte Na-Li countertransport activity have been reported in hypertension and diabetic nephropathy and correlated with increased activity of the renal brush border Na-H exchanger. A relationship between cation exchange activities of blood cells and renal brush border membranes might exist if both were mediated by the same NHE isoform. We generated isoform-specific antibodies to compare the expression of NHE1 and NHE3 in peripheral blood cell membranes and renal cortical membrane vesicles. An NHE1-specific monoclonal antibody reacted with a 199- to 110-kD protein in basolateral membrane fractions isolated from rabbit and rat kidney. NHE1 protein expression was also detected in erythrocytes, platelets, and lymphocytes isolated from rabbit and rat. Two polyclonal antisera generated against nonoverlapping portions of NHE3 reacted with proteins of 82 and 85 kD in brush border membrane fractions isolated from rabbit and rat kidney, respectively, but failed to detect NHE3 expression in blood cells. These data do not support the hypothesis that Na-H exchanger of Na-Li countertransport in blood cells takes place via the renal brush border membrane NHE isoform, namely NHE3.
Rutherford
P A
PA
Department of Medicine, Yale University School of Medicine, New Haven, Conn., USA.
Pizzonia
J H
JH
Biemesderfer
D
D
Abu-Alfa
A
A
Reilly
R
R
Aronson
P S
PS
D000818:Animals; D000911:Antibodies, Monoclonal; D000918:Antibody Specificity; D001773:Blood Cells / Q000737:chemistry*; D001792:Blood Platelets / Q000737:chemistry; D002462:Cell Membrane / Q000737:chemistry; D004910:Erythrocyte Membrane / Q000737:chemistry; D004912:Erythrocytes / Q000737:chemistry; D015151:Immunoblotting; D007668:Kidney / Q000737:chemistry*; D008214:Lymphocytes / Q000737:chemistry; D011817:Rabbits; D051381:Rats; D017207:Rats, Sprague-Dawley; D017923:Sodium-Hydrogen Exchangers / Q000032:analysis*
D000911:Antibodies, Monoclonal; D017923:Sodium-Hydrogen Exchangers