title: Metal ion binding to a zinc finger peptide containing the Cys-X2-Cys-X4-His-X4-Cys domain of a nucleic acid binding protein encoded by the Drosophila Fw-element.
abstract: The metal binding properties of a 18-residue zinc finger peptide containing a CCHC box which reproduces one of the cysteine-rich domains of a putative nucleic acid binding protein encoded by the Fw transposable element from Drosophila melanogaster were investigated through electronic and 1H NMR spectroscopy. Dissociation constants of 2(+/- 1) x 10(-12) M and 4(+/- 1) x 10(-7) M were determined for the Zn2+ and Co2+ adduct, respectively. These values are similar to those for other CCHC-peptides investigated previously, although the length of the spacer between the second cysteine and the histidine apparently exerts some influence on the spectral properties and on the stability of the Co(2+)-peptide adduct. The 1H NMR spectrum of the present Co(2+)-derivative contains a number of well resolved hyperfine-shifted resonances between 350 and -50 ppm which arise from the metal binding residues and nearby groups. These peaks can in principle be profitably exploited to monitor protein-nucleic acid interactions.
authors.affiliation: Department of Chemistry, University of Basilicata, Potenza, Italy.
authors.forename: A
authors.initials: A
authors.lastname: Bavoso
authors.forename: A
authors.initials: A
authors.lastname: Ostuni
authors.forename: G
authors.initials: G
authors.lastname: Battistuzzi
authors.forename: L
authors.initials: L
authors.lastname: Menabue
authors.forename: M
authors.initials: M
authors.lastname: Saladini
authors.forename: M
authors.initials: M
authors.lastname: Sola
mesh_terms: D000818:Animals; D003035:Cobalt / Q000378:metabolism; D003545:Cysteine / Q000737:chemistry / Q000378:metabolism; D004268:DNA-Binding Proteins / Q000737:chemistry*; D004331:Drosophila melanogaster / Q000737:chemistry*; D006639:Histidine / Q000737:chemistry / Q000378:metabolism; D009682:Magnetic Resonance Spectroscopy; D008670:Metals / Q000378:metabolism; D010446:Peptide Fragments / Q000378:metabolism; D011485:Protein Binding; D013053:Spectrophotometry; D015032:Zinc / Q000378:metabolism; D016335:Zinc Fingers
chemical_list: D004268:DNA-Binding Proteins; D008670:Metals; D010446:Peptide Fragments; D003035:Cobalt; D006639:Histidine; D015032:Zinc; D003545:Cysteine