Biochemical and molecular-genetic properties of a cytochrome-c-deficient mutant of Kluyveromyces lactis.
We have isolated a respiration-deficient nuclear mutant of the yeast Kluyveromyces lactis that exhibited diminished levels of all cytochromes and did not grow on glycerol and other nonfermentable carbon sources. The mutant named cyc1 was transformed with a K. lactis genomic library and the DNA fragment conferring its wild-type properties was isolated and sequenced. The sequence of the isolated gene showed extensive homology with other eukaryotic cytochrome-c genes. The highest level of homology, based on the deduced amino acid sequences, was observed between the gene products of K. lactis and Hansenula anomala.
Hikkel
I
I
Comenius University, Department of Microbiology and Virology, Bratislava, Slovak Republic.
Gbelská
Y
Y
Subík
J
J
Lubec
G
G
D000595:Amino Acid Sequence; D000818:Animals; D001059:Apoproteins / Q000172:deficiency* / Q000235:genetics; D002244:Carbon / Q000378:metabolism; D003470:Culture Media / Q000378:metabolism; D003574:Cytochrome c Group / Q000172:deficiency* / Q000235:genetics; D045304:Cytochromes c; D004271:DNA, Fungal / Q000235:genetics; D005285:Fermentation; D005656:Fungal Proteins / Q000235:genetics*; D015723:Gene Library; D005800:Genes, Fungal; D005990:Glycerol / Q000378:metabolism; D006801:Humans; D007716:Kluyveromyces / Q000201:enzymology / Q000235:genetics* / Q000254:growth & development; D008928:Mitochondria / Q000201:enzymology; D008969:Molecular Sequence Data; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid; D013045:Species Specificity
D001059:Apoproteins; D003470:Culture Media; D003574:Cytochrome c Group; D004271:DNA, Fungal; D005656:Fungal Proteins; D002244:Carbon; D045304:Cytochromes c; D005990:Glycerol