title: Active uptake of serotonin as viability parameter of stored platelets.
abstract: The platelet-plasma serotonin (5-HT) exchange in different apheresis products and platelet concentrates (PCs) from buffy coats was studied for quality control over a period of 7 days. In PCs with steadily decreasing pH due to inappropriate gas exchange the platelet 5-HT concentration increases significantly (> 10% positive netto uptake) during the 1st day of storage. Thereafter platelet serotonin drops rapidly with t1/2 = 1 day at pH < 6.5, but platelet counts were constant and 5-HT uptake was inhibited concomitantly. Plasma 5-HT increases as pH decreases. This can be enforced by competitive inhibition of serotonin uptake due to imipramine which also moderates the total 5-HT loss. However, in PC with stable pH the operative 5-HT pump keeps steady-state conditions. The efficacy of the transmembrane uptake mechanism can be adequately monitored by the assessment of the 5-HT platelet/plasma ratio as a new platelet viability parameter.
authors.affiliation: Abteilung Transfusionsmedizin und Transplantationsimmunologie, UKE, Universität Hamburg, Germany.
authors.forename: H P
authors.initials: HP
authors.lastname: Benn
authors.forename: I
authors.initials: I
authors.lastname: Ganschow
authors.forename: R
authors.initials: R
authors.lastname: Fischer
authors.forename: B
authors.initials: B
authors.lastname: Dresow
authors.forename: U
authors.initials: U
authors.lastname: Cassens
authors.forename: R
authors.initials: R
authors.lastname: Dittmer
authors.forename: P
authors.initials: P
authors.lastname: Kühnl
mesh_terms: D001792:Blood Platelets / Q000166:cytology / Q000378:metabolism; D001793:Blood Preservation; D002470:Cell Survival; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007962:Leukocytes; D010983:Plateletpheresis; D012701:Serotonin / Q000097:blood*; D013997:Time Factors
chemical_list: D012701:Serotonin