Polymorphisms in the p53 gene in thyroid tumours and blood samples of children from areas in Belarus.
We present changes in the p53 gene in a group of 70 thyroid tumours and 40 blood samples obtained from children from Belarus. Three thyroid tumours show a polymorphism in exon 6 (codon 213) and 5 tumours show a polymorphism in intron 6, 37 bp upstream to the 5'-end of exon 7. Only one patient has a mutation in exon 7 (codon 258) resulting in an amino acid substitution in the protein p53. The distribution of polymorphisms in the 40 blood samples was as follows: three patients had a polymorphism in exon 6 and two persons had a polymorphism in intron 6. One polymorphism in intron 6 was also found in the group of 30 healthy children from Belarus. The fact that the differences in the sequence in p53 found in the tumours was also seen in the blood of these patients demonstrates that they are polymorphisms not induced by radiation exposure. It is difficult to conclude, if the polymorphisms found by us could be associated with the predisposition to radiation-induced cancer.
Hillebrandt
S
S
Institut für Medizinische Strahlenbiologie, Universitätsklinikum Essen, Germany.
Streffer
C
C
Demidchik
E P
EP
Biko
J
J
Reiners
C
C
D000293:Adolescent; D002291:Carcinoma, Papillary / Q000097:blood / Q000453:epidemiology / Q000209:etiology / Q000235:genetics; D002648:Child; D003043:Cocarcinogenesis; D004252:DNA Mutational Analysis; D004273:DNA, Neoplasm / Q000235:genetics; D004198:Disease Susceptibility; D005091:Exons / Q000235:genetics; D005260:Female; D016158:Genes, p53; D006801:Humans; D007438:Introns / Q000235:genetics; D008297:Male; D009381:Neoplasms, Radiation-Induced / Q000097:blood / Q000453:epidemiology / Q000209:etiology / Q000235:genetics*; D011110:Polymorphism, Genetic; D011210:Power Plants; D018788:Radioactive Hazard Release; D002094:Republic of Belarus / Q000453:epidemiology; D013964:Thyroid Neoplasms / Q000097:blood / Q000453:epidemiology / Q000209:etiology / Q000235:genetics*; D016159:Tumor Suppressor Protein p53 / Q000097:blood*; D014455:Ukraine
D004273:DNA, Neoplasm; D016159:Tumor Suppressor Protein p53