Midkine, a retinoic acid-inducible heparin-binding cytokine, is a novel regulator of intracellular calcium in human neutrophils.
Midkine (MK), which induces chemotaxis of human neutrophils, was found to trigger mobilization of intracellular calcium of these cells. The maximum response was observed 150 sec after exposure to MK, suggesting a complex mechanism in the process. The calcium mobilization was inhibited by herbimycin A, Bordetella pertussis toxin and wortmannin, suggesting that a tyrosine kinase, a G protein-linked receptor and phosphatidyl inositol 3 kinase are involved in the process.
Takada
T
T
Department of Biochemistry, Nagoya University School of Medicine, Japan.
Kinkori
T
T
Muramatsu
H
H
Hayakawa
A
A
Torii
S
S
Muramatsu
T
T
D000730:Androstadienes / Q000494:pharmacology; D016227:Benzoquinones; D002118:Calcium / Q000037:antagonists & inhibitors / Q000378:metabolism*; D002352:Carrier Proteins / Q000037:antagonists & inhibitors / Q000096:biosynthesis / Q000378:metabolism / Q000494:pharmacology*; D002634:Chemotaxis, Leukocyte / Q000187:drug effects; D016207:Cytokines; D005434:Flow Cytometry; D006493:Heparin / Q000378:metabolism; D006801:Humans; D007424:Intracellular Fluid / Q000187:drug effects / Q000378:metabolism*; D047029:Lactams, Macrocyclic; D000078792:Midkine; D009504:Neutrophils / Q000187:drug effects / Q000378:metabolism*; D011809:Quinones / Q000494:pharmacology; D017828:Rifabutin / Q000031:analogs & derivatives; D015398:Signal Transduction / Q000187:drug effects; D014212:Tretinoin / Q000494:pharmacology; D000077191:Wortmannin
D000730:Androstadienes; D016227:Benzoquinones; D002352:Carrier Proteins; D016207:Cytokines; D047029:Lactams, Macrocyclic; D011809:Quinones; D000078792:Midkine; D017828:Rifabutin; D014212:Tretinoin; C020754:herbimycin; D006493:Heparin; D002118:Calcium; D000077191:Wortmannin