Nucleotide sequence of a cDNA clone encoding a Caenorhabditis elegans homolog of mammalian alkyl-dihydroxyacetonephosphate synthase: evolutionary switching of peroxisomal targeting signals.
The nucleotide sequence is reported of a cDNA clone encoding a Caenorhabditis elegans homolog of guinea pig and human alkyl-dihydroxyacetonephosphate synthase. The open reading frame encodes a protein of 597 amino acids which shows extensive homology with the mammalian enzymes (52% identical and about 76% similar in the overlapping region). In contrast to the mammalian enzymes, which carry a consensus peroxisomal targeting signal type 2 in a cleavable N-terminal presequence, this Caenorhabditis elegans homolog carries a consensus peroxisomal targeting signal type 1 (CKL) at its C-terminus. Expression of this protein in an in vitro transcription/translation system yielded a 65 kDa protein. Recombinant aenorhabditis elegans alkyl-DHAP synthase expressed in the yeast Pichia pastoris was enzymatically active.
de Vet
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Centre for Biomembranes and Lipid Enzymology, Utrecht University, The Netherlands.
Prinsen
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van den Bosch
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D019883:Alkyl and Aryl Transferases / Q000737:chemistry*; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D017173:Caenorhabditis elegans / Q000201:enzymology*; D003001:Cloning, Molecular; D004591:Electrophoresis, Polyacrylamide Gel; D015966:Gene Expression Regulation, Fungal; D015801:Helminth Proteins / Q000737:chemistry; D008830:Microbodies / Q000378:metabolism; D008969:Molecular Sequence Data; D010843:Pichia / Q000235:genetics; D014176:Protein Biosynthesis / Q000235:genetics; D021382:Protein Sorting Signals / Q000737:chemistry; D011994:Recombinant Proteins / Q000235:genetics; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D014158:Transcription, Genetic / Q000235:genetics
D015801:Helminth Proteins; D021382:Protein Sorting Signals; D011994:Recombinant Proteins; D019883:Alkyl and Aryl Transferases; C019654:alkylglycerone-phosphate synthase