title: Preeclampsia: immunologic alteration of Nitabuch's membrane? Clinical sequelae.
abstract: OBJECTIVE
  To determine if preeclampsia is an immunologic disease process consistent with a host-vs.-graft reaction by examining differences in immunoglobulin deposition at the maternal fetal interface (Nitabuch's layer) in preeclamptic patients.
  
  
  METHODS
  A prospective study of patients at Lehigh Valley Hospital was conducted between July 1993 and April 1994. Group 1 (study) consisted of 11 primigravid women meeting the criteria for the diagnosis of preeclampsia. Group 2 (control) consisted of 11 primigravid women who had an uncomplicated pregnancy. At delivery, basal plate placental specimens were obtained, fixed, and processed for study by a blinded observer utilizing IgG, IgA, IgM, IgE immunofluorescence techniques. The placenta was then sent to a gynecologic pathologist for blinded evaluation and measurement of Nitabuch's membrane by light microscopy.
  
  
  RESULTS
  Taking the average width of 3 measurements of Nitabuch's membrane by light microscopy revealed no significant difference (group 1, .1839 mm vs. group 2, .1555 mm). Immunofluorescence techniques revealed that the thickness of immunofluorescence of Nitabuch's membrane was significantly greater in the study group vs. the control group (157.48 pixels vs. 63.80 pixels, P = .006, respectively).
  
  
  CONCLUSIONS
  Evaluation of the maternal-fetal interface reveals the deposition of increased immunoglobulins, the physiology of which may be similar to nephropathies as seen in systemic disease processes. The deposition of immunoglobulins may be associated with a common antigen which may point to an immunologic etiology for preeclampsia in some women.
authors.affiliation: Department of Obstetrics and Gynecology, Lehigh University, Allentown, Pennsylvania, USA.
authors.forename: J
authors.initials: J
authors.lastname: Balducci
authors.forename: P M
authors.initials: PM
authors.lastname: Weiss
authors.forename: R O
authors.initials: RO
authors.lastname: Atlas
authors.forename: M F
authors.initials: MF
authors.lastname: Pajarillo
authors.forename: W B
authors.initials: WB
authors.lastname: Dupree
authors.forename: S K
authors.initials: SK
authors.lastname: Klasko
mesh_terms: D015331:Cohort Studies; D005321:Extraembryonic Membranes; D005260:Female; D019084:Fluorescent Antibody Technique, Indirect; D006801:Humans; D007070:Immunoglobulin A / Q000032:analysis; D007074:Immunoglobulin G / Q000032:analysis; D007075:Immunoglobulin M / Q000032:analysis; D010920:Placenta / Q000276:immunology* / Q000473:pathology; D011225:Pre-Eclampsia / Q000276:immunology* / Q000473:pathology; D011247:Pregnancy; D011446:Prospective Studies
chemical_list: D007070:Immunoglobulin A; D007074:Immunoglobulin G; D007075:Immunoglobulin M