title: Role of nitric oxide in the vasopressin-induced corticosterone secretion in rats.
abstract: The presence of nitric oxide synthase (NOS) in hypothalamic structures which control the activity of the hypothalamic-pituitary-adrenal (HPA) axis suggests a role for NO in regulation of ACTH and corticosterone secretion. We investigated the involvement of NO in the corticosterone secretion induced by vasopressin (AVP), a potent coregulator of the HPA activity. AVP injected i.p. was, on a molar basis, considerably more potent than administered intracerebroventricularly in inducing corticosterone secretion. This finding suggests a preferential action of AVP on pituitary corticotrop receptors, but not on central structures involved in stimulation of the HPA axis. Dexamethasone given before AVP totally abolished the AVP-elicited corticosterone response by a feedback mechanism and/or inhibition of the phospholipase A2 activity and prostaglandin synthesis. Pretreatment with the NOS inhibitors L-NAME and L-NNA augmented significantly and to a similar extent the corticosterone response to AVP administered both systemically and centrally and L-NNA was found to be more potent in this respect. Pretreatment with L-arginine markedly reduced the AVP-induced corticosterone response. These results suggest that endogenous nitric oxide is significantly involved in the AVP-elicited corticosterone secretion and NO-induced alterations in the prostaglandin synthesis may participate in this action.
authors.affiliation: Department of Physiology, Institute of Pharmacology, Polish Academy of Sciences, Cracow, Poland.
authors.forename: J
authors.initials: J
authors.lastname: Bugajski
authors.forename: A
authors.initials: A
authors.lastname: Gadek-Michalska
authors.forename: A
authors.initials: A
authors.lastname: Ołowska
authors.forename: J
authors.initials: J
authors.lastname: Borycz
authors.forename: R
authors.initials: R
authors.lastname: Głód
mesh_terms: D000818:Animals; D003345:Corticosterone / Q000378:metabolism*; D007030:Hypothalamo-Hypophyseal System / Q000187:drug effects / Q000502:physiology*; D007276:Injections, Intraventricular; D008297:Male; D009569:Nitric Oxide / Q000502:physiology*; D010913:Pituitary-Adrenal System / Q000187:drug effects / Q000502:physiology*; D051381:Rats; D017208:Rats, Wistar; D014667:Vasopressins / Q000494:pharmacology
chemical_list: D014667:Vasopressins; D009569:Nitric Oxide; D003345:Corticosterone