title: Simultaneous analysis of lignocaine and bupivacaine enantiomers in plasma by high-performance liquid chromatography.
abstract: A sensitive analytical procedure is described for the simultaneous determination of lignocaine and the enantiomers of bupivacaine in biological fluids using diazepam as an internal standard. After solvent extraction into hexane, the local anaesthetics were separated using an alpha1-acid glycoprotein (AGP) column and detected at 214 nm. Calibration curves were linear (r2>0.99) in the concentration range of 5 to 500 ng/ml for the enantiomers of bupivacaine and 12.5 to 1000 ng/ml for lignocaine. The corresponding limits of detection were 4 ng/ml and 10 ng/ml, respectively. The method was applied to the analysis of plasma from a healthy woman undergoing tubal ligation.
authors.affiliation: School of Pharmacy, University of Otago, Dunedin, New Zealand.
authors.forename: I
authors.initials: I
authors.lastname: Abraham
authors.forename: J P
authors.initials: JP
authors.lastname: Fawcett
authors.forename: J
authors.initials: J
authors.lastname: Kennedy
authors.forename: A
authors.initials: A
authors.lastname: Kumar
authors.forename: R
authors.initials: R
authors.lastname: Ledger
mesh_terms: D000779:Anesthetics, Local / Q000008:administration & dosage / Q000097:blood* / Q000737:chemistry / Q000493:pharmacokinetics; D002045:Bupivacaine / Q000008:administration & dosage / Q000097:blood* / Q000737:chemistry / Q000493:pharmacokinetics; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D002940:Circadian Rhythm; D005260:Female; D006801:Humans; D007279:Injections, Subcutaneous; D008012:Lidocaine / Q000008:administration & dosage / Q000097:blood* / Q000493:pharmacokinetics; D015203:Reproducibility of Results; D013237:Stereoisomerism; D013246:Sterilization, Tubal; D013997:Time Factors
chemical_list: D000779:Anesthetics, Local; D008012:Lidocaine; D002045:Bupivacaine