title: Differential regulation of genes encoding synaptic proteins by the Oct-2 transcription factor.
abstract: In order to investigate the effect of the Oct-2 POU family transcription factor on the regulation of genes encoding synaptic proteins, we have used cell lines in which the level of Oct-2 has been greatly reduced using an antisense approach. The reduced Oct-2 level results in enhanced expression of SNAP-25 and synapsin I, indicating that the genes encoding these proteins are normally repressed by Oct-2 in neuronal cells. In contrast, no alteration was observed in the levels of the synaptic proteins, synaptophysin and synaptotagmin. Although the neuronal forms of Oct-2 can repress the synapsin I promoter in co-transfection experiments, indicating that they have a direct effect on the expression of this gene, they have no effect on the activity of the SNAP-25 promoter, indicating that the effect of Oct-2 on this gene is likely to be indirect. These effects are discussed in terms of the differential effect of Oct-2 and the related POU family transcription factor Brn-3a, on the promoters of genes encoding different synaptic proteins.
authors.affiliation: Department of Molecular Pathology, University College Medical School, London, UK.
authors.forename: Z C
authors.initials: ZC
authors.lastname: Deans
authors.forename: S J
authors.initials: SJ
authors.lastname: Dawson
authors.forename: M W
authors.initials: MW
authors.lastname: Kilimann
authors.forename: D
authors.initials: D
authors.lastname: Wallace
authors.forename: M C
authors.initials: MC
authors.lastname: Wilson
authors.forename: D S
authors.initials: DS
authors.lastname: Latchman
mesh_terms: D000818:Animals; D002135:Calcium-Binding Proteins; D002460:Cell Line; D006224:Cricetinae; D004268:DNA-Binding Proteins / Q000096:biosynthesis / Q000378:metabolism*; D005786:Gene Expression Regulation; D008562:Membrane Glycoproteins / Q000096:biosynthesis; D008565:Membrane Proteins; D009419:Nerve Tissue Proteins / Q000096:biosynthesis*; D009474:Neurons; D011401:Promoter Regions, Genetic; D011994:Recombinant Proteins / Q000096:biosynthesis; D013569:Synapses / Q000378:metabolism*; D016704:Synapsins / Q000096:biosynthesis*; D016708:Synaptophysin / Q000096:biosynthesis; D050825:Synaptosomal-Associated Protein 25; D050857:Synaptotagmins; D014157:Transcription Factors / Q000096:biosynthesis / Q000378:metabolism*; D014162:Transfection
chemical_list: D002135:Calcium-Binding Proteins; D004268:DNA-Binding Proteins; D008562:Membrane Glycoproteins; D008565:Membrane Proteins; D009419:Nerve Tissue Proteins; D011994:Recombinant Proteins; D016704:Synapsins; D016708:Synaptophysin; D050825:Synaptosomal-Associated Protein 25; D014157:Transcription Factors; D050857:Synaptotagmins