[Features of growth and formation of extracellular proteinases of Bifidobacterium adolescentis 94-BIM].
Growth characteristics of a new bifidobacterial strain, Bifidobacterium adolescentis 94-BIM, were determined during cultivation in various nutrient media. The strain was obtained through selection by the criterion of proteolytic activity and was shown to synthesize acidic, neutral, and alkaline extracellular proteinases in all of the growth media tested. The growth yield of the strain varied from 2.9 x 10(8) to 1.7 x 10(10) CFU/ml with acidogenesis of up to 131 degrees T. The most rapid growth and accumulation of extracellular proteinases were observed during the first 24 h of cultivation. The maximum growth rate (0.144 h-1) was observed in the ninth hour of cultivation, whereas the most rapid accumulation of acidic, neutral, and alkaline proteinases (at rates of 53, 88.8, and 59.2 units/(mg h), respectively) occurred in the sixth hour of cultivation.
Samartsev
A A
AA
Astanovich
N I
NI
Novik
G I
GI
D001644:Bifidobacterium / Q000201:enzymology* / Q000254:growth & development*; D003470:Culture Media; D010450:Endopeptidases / Q000096:biosynthesis*; D006863:Hydrogen-Ion Concentration; D007700:Kinetics
D003470:Culture Media; D010450:Endopeptidases