REP-PCR analysis of Pasteurella multocida isolates that cause haemorrhagic septicaemia.
Amplification of multiple P multocida genomic DNA fragments by outwardly-directed primers based on the repetitive extragenic palindromic (REP) consensus sequence, generated complex profiles in a PCR-based fingerprinting method known as REP-PCR. Polymorphisms within REP-PCR profiles were used to characterise 38 isolates of P multocida. The high degree of homogeneity observed among haemorrhagic septicaemia (HS) strains of serotype B and E provided evidence of a disease-associated REP profile that may serve as a novel method for the identification of HS strains regardless of serotype. REP-PCR profiles of other P multocida serotypes were highly variable, illustrating the potential of this technique for the molecular fingerprinting of fowl cholera or atrophic rhinitis isolates. A specific amplified REP fragment was isolated and used to probe membrane-bound digested P multocida genomic DNA. Hybridisation patterns not only distinguished HS-causing isolates from non-HS P multocida, but also demonstrated a degree of relatedness between HS and HS-like strains.
Townsend
K M
KM
UWA Department of Pathology, Q.E. II Medical Centre, Nedlands, Australia. kirsty.townsend@mailbox.uq.edu.au
Dawkins
H J
HJ
Papadimitriou
J M
JM
D000818:Animals; D001483:Base Sequence; D001715:Bird Diseases / Q000209:etiology / Q000382:microbiology; D001717:Birds; D016164:Bison; D002020:Buffaloes; D002417:Cattle; D016172:DNA Fingerprinting / Q000662:veterinary; D053938:DNA Fragmentation; D004269:DNA, Bacterial / Q000032:analysis / Q000737:chemistry / Q000235:genetics; D003670:Deer; D006483:Hemorrhagic Septicemia / Q000209:etiology / Q000662:veterinary*; D006801:Humans; D016979:Pasteurella multocida / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification; D016133:Polymerase Chain Reaction / Q000379:methods / Q000662:veterinary*; D011201:Poultry Diseases / Q000209:etiology / Q000382:microbiology; D013552:Swine; D013553:Swine Diseases / Q000209:etiology / Q000382:microbiology; D014422:Turkeys
D004269:DNA, Bacterial