title: Beta2 toxin, a novel toxin produced by Clostridium perfringens.
abstract: A novel toxin (Beta2) and its gene were characterized from a Clostridium perfringens strain isolated from a piglet with necrotic enteritis. At the amino-acid level, Beta2 toxin (27670 Da) has no significant homology with the previously identified Beta toxin (called Beta1) (34861 kDa) from C. perfringens type B NCTC8533 ( Hunter, S.E.C., Brown, J.E., Oyston, P.C.F., Sakurai, J., Titball, R.W., 1993. Molecular genetic analysis of beta-toxin of Clostridium perfringens reveals sequence homology with alpha-toxin, gamma-toxin, and leukocidin of Staphylococcus aureus. Infect. Immun. 61, 3958-3965). Both Beta1 and Beta2 toxins were lethal for mice and cytotoxic for the cell line 1407, inducing cell rounding and lysis without affecting the actin cytoskeleton. The genes encoding Beta1 and Beta2 toxins have been localized in unlinked loci in large plasmids of C. perfringens. In addition, Beta2 toxin-producing C. perfringens strains were found to be associated with animal diseases such as necrotic enteritis in piglets and enterocolitis in horses.
authors.affiliation: Unité des Toxines Microbiennes, Institut Pasteur, Paris, France.
authors.forename: M
authors.initials: M
authors.lastname: Gibert
authors.forename: C
authors.initials: C
authors.lastname: Jolivet-Reynaud
authors.forename: M R
authors.initials: MR
authors.lastname: Popoff
authors.forename: C
authors.initials: C
authors.lastname: Jolivet-Renaud
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001426:Bacterial Proteins; D001427:Bacterial Toxins / Q000737:chemistry / Q000235:genetics* / Q000633:toxicity; D001483:Base Sequence; D002874:Chromosome Mapping; D003001:Cloning, Molecular; D003016:Clostridium perfringens / Q000235:genetics*; D004269:DNA, Bacterial; D051379:Mice; D008969:Molecular Sequence Data; D013552:Swine
chemical_list: D001426:Bacterial Proteins; D001427:Bacterial Toxins; D004269:DNA, Bacterial; C109896:cpb2 protein, Clostridium perfringens