title: Cytogenetic analysis using simultaneous and sequential fluorescence in situ hybridization.
abstract: A sequential fluorescence in situ hybridization (FISH) technique is described. This method allows the detection of up to eighteen chromosome pairs in consecutive hybridizations (8 steps) on the same metaphase using centromeric, whole chromosome painting, and single copy DNA probes with different fluorochromes. The technique may be used with diagnostic purposes in cases with poor cytogenetic material.
authors.affiliation: Department de Biologia cel-lular i Fisiologia, Universitat Autònoma de Barcelona, Bellaterra, Spain.
authors.forename: T
authors.initials: T
authors.lastname: Escudero
authors.forename: C
authors.initials: C
authors.lastname: Fuster
authors.forename: M D
authors.initials: MD
authors.lastname: Coll
authors.forename: J
authors.initials: J
authors.lastname: Egozcue
mesh_terms: D002478:Cells, Cultured; D003582:Cytogenetics; D006801:Humans; D017404:In Situ Hybridization, Fluorescence / Q000379:methods*; D009369:Neoplasms / Q000235:genetics; D011296:Prenatal Diagnosis