Automated bone marrow concentration using the Fresenius AS 104 blood cell separator.
We concentrated freshly taken bone marrow (BM) pooled from 21 patients from an original 1.265 ml (+/- 537 ml) down to 128 ml (+/- 36 ml) within 40-70 min with a modified version of the 'grancollect protocol' on the Fresenius AS 104 blood-cell separator using the P1-Y set. An average of 47% (+/- 21%) fo the initially present mononuclear cells and 68% (+/- 47%) of the colony-forming cells could be obtained in the concentrate. The erythrocyte concentration was reduced to 7% (+/- 4%) of the original amount. The technique described is very effective and makes is possible to obtain a BM cell population that is suitable for both immunomagnetic purging and cryopreservation or transplantation, e.g. in the case of blood group incompatibility.
Zingsem
J
J
Abteilung für Transfusionsmedizin, Friedrich-Alexander-Universität Erlangen.
Weisbach
V
V
Zimmermann
R
R
Zeiler
T
T
Mitschulat
H
H
Beyer
J
J
Eckstein
R
R
D001331:Automation / Q000295:instrumentation / Q000379:methods; D001854:Bone Marrow Cells; D016465:Bone Marrow Purging / Q000379:methods; D002469:Cell Separation / Q000295:instrumentation* / Q000379:methods; D015925:Cryopreservation / Q000379:methods; D004867:Equipment Design; D006098:Granulocytes; D006801:Humans; D018189:Immunomagnetic Separation; D009373:Neoplasms, Germ Cell and Embryonal / Q000473:pathology / Q000628:therapy*; D054198:Precursor Cell Lymphoblastic Leukemia-Lymphoma / Q000473:pathology