title: Independent folding and ligand specificity of the C2 calcium-dependent lipid binding domain of cytosolic phospholipase A2.
abstract: The Ca(2+)-dependent lipid binding domain of the 85-kDa cytosolic phospholipase A2 (cPLA2) is a homolog of C2 domains present in protein kinase C, synaptotagmin, and numerous other proteins involved in signal transduction. NH2-terminal fragments of cPLA2 spanning the C2 domain were expressed as inclusion bodies in Escherichia coli, extracted with solvent to remove phospholipids, and refolded to yield a domain capable of binding phospholipid vesicles in a Ca(2+)-dependent manner. Unlike other C2 domains characterized to date, the cPLA2 C2 domain bound preferentially to vesicles comprised of phosphatidylcholine in response to physiological concentrations of Ca2+. Binding of the cPLA2 C2 domain to vesicles in the presence of excess Ca2+ chelator was induced by high concentrations of salts that promote hydrophobic interactions. Despite the selective hydrolysis of arachidonyl-containing phospholipid vesicles by cPLA2, the cPLA2 C2 domain did not discriminate among phospholipid vesicles containing saturated or unsaturated sn-2 fatty acyl chains. Moreover, the cPLA2 C2 domain bound to phospholipid vesicles containing sn-1 and -2 ether linkages and sphingomyelin at Ca2+ concentrations that caused binding to vesicles containing ester linkages, demonstrating that the carbonyl oxygens of the sn-1 and -2 ester linkage are not critical for binding. These results suggest that the cPLA2 C2 domain interacts primarily with the headgroup of the phospholipid. The cPLA2 C2 domain displayed selectivity among group IIA cations, preferring Ca2+ approximately 50-fold over Sr2+ and nearly 10,000-fold over Ba2+ for vesicle binding. No binding to vesicles was observed in the presence of greater than 10 mM Mg2+. Such strong selectivity for Ca2+ over Mg2+ reinforces the view that C2 domains link second messenger Ca2+ to signal transduction events at the membrane.
authors.affiliation: Small Molecule Drug Discovery Group, Genetics Institute, Inc., Cambridge, Massachusetts 02140, USA.
authors.forename: E A
authors.initials: EA
authors.lastname: Nalefski
authors.forename: T
authors.initials: T
authors.lastname: McDonagh
authors.forename: W
authors.initials: W
authors.lastname: Somers
authors.forename: J
authors.initials: J
authors.lastname: Seehra
authors.forename: J J
authors.initials: JJ
authors.lastname: Falke
authors.forename: J D
authors.initials: JD
authors.lastname: Clark
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001464:Barium / Q000378:metabolism; D001665:Binding Sites; D016466:CHO Cells; D019556:COS Cells; D002118:Calcium / Q000378:metabolism*; D006224:Cricetinae; D008024:Ligands; D008274:Magnesium / Q000378:metabolism; D008969:Molecular Sequence Data; D010741:Phospholipases A / Q000378:metabolism*; D054467:Phospholipases A2; D017510:Protein Folding; D016415:Sequence Alignment; D013324:Strontium / Q000378:metabolism; D013329:Structure-Activity Relationship; D013379:Substrate Specificity
chemical_list: D008024:Ligands; D001464:Barium; D010741:Phospholipases A; D054467:Phospholipases A2; D008274:Magnesium; D002118:Calcium; D013324:Strontium