Characteristics of nucleotide sequences flanking the trans-spliced leader SL1 exon in Dirofilaria immitis, Brugia malayi, and Brugia pahangi.
Nucleotide sequences surrounding the trans-spliced leader SL1 exon in the 5S rRNA gene spacer regions of Dirofilaria immitis, Brugia malayi, and B. pahangi were determined after PCR amplification, aligned with the genus Onchocerca for comparison, and used for the prediction of secondary structures. The nucleotide sequence of this region in B. pahangi was first shown in the present study. Hypothetical secondary structures of the spacer region suggested that the SL1 transcript is capable to form a stable stem-loop structure which may render transposition of the SL1 sequence to mRNA molecules. A homologous sequence to Sm-binding site was assigned on a bulge loop. No significant difference was observed in adult worms of D. immitis irrespective of sex or location. No difference was apparent between the two species in genus Brugia.
Harasawa
R
R
Animal Center for Biomedical Research, Faculty of Medicine, University of Tokyo, Japan.
Maeda
R
R
Nogami
S
S
Nakagaki
K
K
Yoshida
M
M
Kataoka
Y
Y
Kobayashi
H
H
Katae
H
H
Hayashi
Y
Y
D000818:Animals; D000947:Antigens, Helminth / Q000032:analysis / Q000276:immunology; D001483:Base Sequence; D017178:Brugia malayi / Q000235:genetics* / Q000276:immunology; D017179:Brugia pahangi / Q000235:genetics* / Q000276:immunology; D003429:Cross Reactions; D018509:DNA, Helminth / Q000032:analysis / Q000737:chemistry / Q000235:genetics*; D004183:Dirofilaria immitis / Q000235:genetics* / Q000276:immunology; D005091:Exons / Q000235:genetics*; D005260:Female; D005784:Gene Amplification; D008297:Male; D008969:Molecular Sequence Data; D009854:Onchocerca / Q000235:genetics; D016133:Polymerase Chain Reaction / Q000662:veterinary; D021382:Protein Sorting Signals / Q000032:analysis / Q000737:chemistry / Q000235:genetics*; D012333:RNA, Messenger / Q000032:analysis / Q000737:chemistry / Q000235:genetics; D012341:RNA, Ribosomal, 5S / Q000032:analysis / Q000737:chemistry / Q000235:genetics; D012689:Sequence Homology, Nucleic Acid; D014158:Transcription, Genetic
D000947:Antigens, Helminth; D018509:DNA, Helminth; D021382:Protein Sorting Signals; D012333:RNA, Messenger; D012341:RNA, Ribosomal, 5S