Effect of arsenite, arsenate, and the herbicide monosodium methyl arsonate (MSMA) on hepatic metallothionein expression and lipid peroxidation in channel catfish.
Arsenic exerts its toxicity by the generation of reactive oxygen intermediates which caused lipid peroxidation and cellular damage. Metallothioneins (MTs) have been shown to be induced by oxidative stress and act as scavengers of reactive oxygen intermediates. Thus, hepatic MT was examined in channel catfish treated with the herbicide monosodium methyl arsonate (MSMA) and compared to equal doses of trivalent and pentavalent arsenic. Fish were exposed to 0.01, 0.1, and 1.0 mg/L of each compound for 1 week by static renewal. Hepatic MT was measured by the cadmium/hemoglobin (Cd/Hb) saturation assay, ELISA using antibodies raised against the first 10 amino acids of piscine MT, and Northern blot analysis using a cDNA encoding winter flounder hepatic MT. Cd/Hb and ELISA measurements of low molecular weight fractions from the hepatic cytosolic component of fish exposed to MSMA revealed a dose dependent increase in MT. MTs and MT mRNA of fish receiving the 1.0 mg/L dose were significantly induced vs control. Responses to arsenate exposure were more variable, but showed a trend toward a dose-dependent induction of MT and MT mRNA. MT mRNA and protein also showed a dose-dependent increase with arsenite exposure with no significant differences with untreated animals. Hepatic lipid peroxidation (as determined by TBARS) and glutathione was unaltered by any of the arsenical treatments. Thus, the lack of correlation between oxidative stress and MT expression suggest MT may not be a reliable indicator of oxidative stress. In addition, the induction of hepatic MT by various forms of As does not appear to be mediated through an oxidative stress mechanism in the liver.
Schlenk
D
D
Department of Pharmacology, School of Pharmacy, University of Mississippi, MS 38677, USA. dschlenk@olemiss.edu
Wolford
L
L
Chelius
M
M
Steevens
J
J
Chan
K M
KM
D000818:Animals; D001149:Arsenates / Q000633:toxicity*; D020261:Arsenic Poisoning; D001152:Arsenicals; D018053:Arsenites / Q000633:toxicity*; D019256:Cadmium Chloride / Q000494:pharmacology; D019327:Copper Sulfate / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D004797:Enzyme-Linked Immunosorbent Assay; D005978:Glutathione / Q000032:analysis; D006540:Herbicides / Q000633:toxicity*; D007059:Ictaluridae / Q000378:metabolism; D015227:Lipid Peroxidation / Q000187:drug effects*; D008099:Liver / Q000187:drug effects* / Q000378:metabolism; D008297:Male; D008668:Metallothionein / Q000096:biosynthesis* / Q000235:genetics; D017670:Sodium Compounds / Q000633:toxicity*; D019287:Zinc Sulfate / Q000494:pharmacology
D001149:Arsenates; D001152:Arsenicals; D018053:Arsenites; D006540:Herbicides; D017670:Sodium Compounds; C017947:sodium arsenite; D019287:Zinc Sulfate; C009277:sodium arsenate; D008668:Metallothionein; D005978:Glutathione; C020300:monomethylarsonic acid; D019256:Cadmium Chloride; D019327:Copper Sulfate