Measurement of chemiluminescence from neutrophils in a 96-well microplate using Lumi Box U-800 II.
We have developed a microplate photon counting system based on a cooled charge-coupled device (Lumi Box U-800 II) jointly with Maikurotekku Nition Company (Chiba, Japan). The system makes it possible to quantify chemiluminescence (CL) in a 96-well microplate automatically and simultaneously in a single experiment. We studied the measurement conditions for a luminol-dependent CL assay from neutrophils stimulated with opsonized zymosan (OZ) using this system. Parameters examined included the effect of OZ dose per well, mixing speed, mixing time and detection time on CL responses. The results indicated that this system allows the measurement of CL from phagocytes on a large number of samples using small amounts of sample and regents.
Kikuchi
T
T
Department of Hygiene, Hirosaki University School of Medicine, Aomori, Japan.
Suzuki
K
K
Abe
T
T
Satoh
H
H
Endoh
T
T
Hasegawa
H
H
Nakaji
S
S
Sugawara
K
K
Kumae
T
T
D000328:Adult; D004867:Equipment Design; D006801:Humans; D066298:In Vitro Techniques; D007700:Kinetics; D008163:Luminescent Measurements; D008838:Microcomputers; D009504:Neutrophils / Q000502:physiology*; D010783:Photometry / Q000295:instrumentation* / Q000379:methods; D015203:Reproducibility of Results