title: Nonequilibrium response spectroscopy of voltage-sensitive ion channel gating.
abstract: We describe a new electrophysiological technique called nonequilibrium response spectroscopy, which involves application of rapidly fluctuating (as high as 14 kHz) large-amplitude voltage clamp waveforms to ion channels. As a consequence of the irreversible (in the sense of Carnot) exchange of energy between the fluctuating field and the channel protein, the gating response is exquisitely sensitive to features of the kinetics that are difficult or impossible to adequately resolve by means of traditional stepped potential protocols. Here we focus on the application of dichotomous (telegraph) noise voltage fluctuations, a broadband Markovian colored noise that fluctuates between two values. Because Markov kinetic models of channel gating can be embedded within higher-dimensional Markov models that take into account the effects of the voltage fluctuations, many features of the response of the channels can be calculated algebraically. This makes dichotomous noise and its generalizations uniquely suitable for model selection and kinetic analysis. Although we describe its application to macroscopic ionic current measurements, the nonequilibrium response method can also be applied to gating and single channel current recording techniques. We show how data from the human cardiac isoform (hH1a) of the Na+ channel expressed in mammalian cells can be acquired and analyzed, and how these data reveal hidden aspects of the molecular kinetics that are not revealed by conventional methods.
authors.affiliation: Department of Pharmacological and Physiological Sciences, The University of Chicago, Illinois 60637, USA. mmillon@kam.uchicago.edu
authors.forename: M M
authors.initials: MM
authors.lastname: Millonas
authors.forename: D A
authors.initials: DA
authors.lastname: Hanck
mesh_terms: D002460:Cell Line; D004594:Electrophysiology; D006321:Heart / Q000502:physiology; D006801:Humans; D015640:Ion Channel Gating / Q000502:physiology*; D007700:Kinetics; D008390:Markov Chains; D008954:Models, Biological; D009010:Monte Carlo Method; D011994:Recombinant Proteins / Q000096:biosynthesis; D015222:Sodium Channels / Q000096:biosynthesis / Q000502:physiology*; D014162:Transfection
chemical_list: D011994:Recombinant Proteins; D015222:Sodium Channels