Induction of human osteoclast-like cells by treatment of blood monocytes with anti-fusion regulatory protein-1/CD98 monoclonal antibodies.
We have developed a new and simple system of human osteoclast formation by fusing peripheral blood monocytes with anti-Fusion Regulatory Protein-1 (anti-FRP-1) monoclonal antibody (mAb). When human blood monocytes were cultured in the presence of anti-FRP-1/CD98 mAbs, polykaryocytes began to appear at approximately 15 h and increased in size with time until 3-4 days of incubation with anti-FRP-1 mAb. These fused cells showed positive staining in tartrate-resistant acid phosphatase, possessed numerous calcitonin receptors, and were capable of bone resorption. These results strongly suggest that anti-FRP-1 antibody-induced multinucleated cells are osteoclasts. Furthermore, FRP-1 antigens were detected in osteoclasts isolated from human bone and in the osteoclast-like cells obtained from human giant cell tumors of bone.
Higuchi
S
S
Department of Microbiology, Mie University School of Medicine, Japan.
Tabata
N
N
Tajima
M
M
Ito
M
M
Tsurudome
M
M
Sudo
A
A
Uchida
A
A
Ito
Y
Y
D000135:Acid Phosphatase; D000596:Amino Acids / Q000032:analysis; D000911:Antibodies, Monoclonal / Q000494:pharmacology*; D015703:Antigens, CD / Q000032:analysis / Q000276:immunology*; D000954:Antigens, Surface / Q000032:analysis; D002352:Carrier Proteins / Q000032:analysis / Q000276:immunology*; D002478:Cells, Cultured; D002648:Child; D005269:Femur / Q000166:cytology; D027261:Fusion Regulatory Protein-1; D005870:Giant Cell Tumors / Q000276:immunology / Q000473:pathology; D015726:Giant Cells / Q000276:immunology / Q000473:pathology; D006801:Humans; D007527:Isoenzymes; D009000:Monocytes / Q000166:cytology* / Q000276:immunology*; D010010:Osteoclasts / Q000166:cytology* / Q000276:immunology*; D018003:Receptors, Calcitonin / Q000032:analysis; D013194:Staining and Labeling; D000071681:Tartrate-Resistant Acid Phosphatase
D000596:Amino Acids; D000911:Antibodies, Monoclonal; D015703:Antigens, CD; D000954:Antigens, Surface; D002352:Carrier Proteins; D027261:Fusion Regulatory Protein-1; D007527:Isoenzymes; D018003:Receptors, Calcitonin; C015484:pyridinoline; D000135:Acid Phosphatase; D000071681:Tartrate-Resistant Acid Phosphatase