Culture in the presence of sugars increases activity of multi-drug efflux transporter on Haloferax volcanii.
We found that when a growth medium contained glucose, wild-type cells of Haloferax volcanii were able to grow even in the presence of doxorubicin (DOX), an anti-cancer reagent, whereas they usually cannot grow in its presence. The reason was that cells grown in the presence of glucose (glucose-grown cells) showed high multi-drug efflux activity even though the growth medium contained no DOX or substrates of the transporter. This transporter was ATP-driven and the elevation of efflux activity was not due to an increase in intracellular ATP contents. The activity was increased not only by glucose but also by sugars that could be metabolized.
Miyauchi
S
S
Laboratory of Biophysical Chemistry, Faculty of Pharmaceutical Sciences, Hokkaido University, Sapporo, Japan.
Tanabu
S
S
Abe
A
A
Okumura
R
R
Kamo
N
N
D020168:ATP Binding Cassette Transporter, Subfamily B, Member 1 / Q000378:metabolism*; D000255:Adenosine Triphosphate / Q000378:metabolism; D000903:Antibiotics, Antineoplastic / Q000378:metabolism / Q000494:pharmacology; D002241:Carbohydrates / Q000494:pharmacology*; D003470:Culture Media; D004024:Dicyclohexylcarbodiimide / Q000494:pharmacology; D004317:Doxorubicin / Q000378:metabolism / Q000494:pharmacology; D005947:Glucose / Q000378:metabolism; D019622:Haloferax volcanii / Q000187:drug effects / Q000378:metabolism*; D008564:Membrane Potentials / Q000502:physiology
D020168:ATP Binding Cassette Transporter, Subfamily B, Member 1; D000903:Antibiotics, Antineoplastic; D002241:Carbohydrates; D003470:Culture Media; D004024:Dicyclohexylcarbodiimide; D004317:Doxorubicin; D000255:Adenosine Triphosphate; D005947:Glucose