title: Identification of a renal proximal tubular epithelial antigen coexpressed on leukocytes.
abstract: Renal tubular epithelial cells (TEC) and leukocytes express distinct cell adhesion molecules and integrins in common. To identify additional proximal TEC molecules that are coexpressed on leukocytes, we have generated a panel of monoclonal antibodies (mAb) by immunization of rats with a mouse T cell line. One of these mAb (I-72) recognizes a 210-kD antigen on cultured TEC. The staining pattern in several cell lines allowed exclusion of known TEC surface molecules as being the antigens recognized by the I-72 mAb. Immunofluorescence staining of mouse kidney with the I-72 mAb demonstrated prominent expression on the apical but not the basolateral side of proximal tubules, predominantly in the S1 segment. I-72 was also expressed in the loop of Henle, the collecting duct, and on endothelial cells of intrarenal vessels. We have thus generated a mAb which identifies an unusual renal proximal tubular brush border molecule that is coexpressed on T cells. Molecular structure and the function of I-72 need to be defined in further studies.
authors.affiliation: Physiological Institute, University Zürich-Irchel, Switzerland.
authors.forename: X
authors.initials: X
authors.lastname: Fan
authors.forename: B
authors.initials: B
authors.lastname: Oertli
authors.forename: R P
authors.initials: RP
authors.lastname: Wüthrich
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal; D000941:Antigens / Q000032:analysis*; D015153:Blotting, Western; D002478:Cells, Cultured; D004591:Electrophoresis, Polyacrylamide Gel; D004730:Endothelium, Vascular / Q000276:immunology; D004848:Epithelium / Q000276:immunology; D005260:Female; D005455:Fluorescent Antibody Technique; D007371:Interferon-gamma / Q000494:pharmacology; D007687:Kidney Tubules, Proximal / Q000276:immunology*; D007962:Leukocytes / Q000276:immunology*; D051379:Mice; D051381:Rats; D017207:Rats, Sprague-Dawley; D014018:Tissue Distribution; D014409:Tumor Necrosis Factor-alpha / Q000494:pharmacology
chemical_list: D000911:Antibodies, Monoclonal; D000941:Antigens; D014409:Tumor Necrosis Factor-alpha; D007371:Interferon-gamma