Effects of transfection of p210bcr-abl and bcr-v-abl into the factor-dependent human leukemia cell line HSM-911.
In order to clarify the action of the bcr-abl, a growth factor dependent human leukemic cell line (HSM-911) was transfected with p210bcr-abl or bcr-v-abl by electroporation. The cells transfected with bcr-v-abl, but not the cells transfected with p210bcr-abl, became growth factor independent. Some clones of the cells transfected with p210bcr-abl demonstrated cellular maturation (nuclear segmentation, becoming positive for naphthol ASD chloroacetate esterase, the disappearance of CD34 expression and the appearance of glycophorin A and CD10 expression). Moreover, these clones transfected with p210bcr-abl demonstrated apoptosis (increased expression of Fas and DNA ladder formation suggesting apoptotic DNA fragmentation). These findings demonstrated the different actions of p210 bcr-abl and bcr-v-abl, the former of which gave the cells the characteristics of maturation like the cells from chronic myelogenous leukemia, and the latter of which rendered the cells grow autonomously.
Takahashi
M
M
Department of Medical Technology, College of Biomedical Technology, Niigata University, Japan.
Shigeno
N
N
Takahashi
H
H
Suzuki
N
N
Masuko
M
M
Nikkuni
K
K
Toba
K
K
Furukawa
T
T
Aoki
S
S
Kishi
K
K
Koike
T
T
Aizawa
Y
Y
D017209:Apoptosis / Q000502:physiology; D002455:Cell Division / Q000502:physiology; D016044:Fusion Proteins, bcr-abl / Q000235:genetics* / Q000494:pharmacology; D006801:Humans; D015456:Leukemia, Biphenotypic, Acute / Q000235:genetics* / Q000473:pathology*; D014162:Transfection; D014407:Tumor Cells, Cultured
D016044:Fusion Proteins, bcr-abl