Gene expression of trehalase during post-dormant development of the brine shrimp, Artemia: comparison of the two species.
Based on a homology screening approach, two degenerate oligonucleotides were employed as primers in a polymerase chain reaction to amplify a fragment of DNA encoding trehalase with a template of cDNA derived from embryos of American Artemia. Sequence analysis revealed that the fragment was composed of 228 bp comprising 76 amino acids, and highly homologous to trehalases of Tenebrio molitor (mealworm beetle), rabbit, Caenorhabditis elegans, Bombyx mori (silkworm) and Escherichia coli treA and treF (58-38%, in order of description). This fragment was used as a hybridization probe. A Northern blot analysis on American Artemia showed three transcripts of 5.0, 2.7 and 2.2 kb, and the two larger transcripts were also detected in Chinese Artemia. The developmental profile of the gene expression and the trehalase activity suggest that the transcripts of 5.0 and (or) 2.7 kb in both Artemia may be directly or indirectly related to translation of the trehalase. A Southern blot analysis on both Artemia suggested the existence of two highly homologous genes or one gene having an intron within the region where the probe binds in their haploid genome.
Nambu
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Department of Medical Technology I, School of Health Sciences, University of Occupational and Environmental Health, Japan.
Tanaka
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Nambu
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D000595:Amino Acid Sequence; D000818:Animals; D001156:Artemia / Q000196:embryology / Q000201:enzymology*; D015139:Blotting, Southern; D017931:DNA Primers; D015342:DNA Probes; D018507:Gene Expression Regulation, Developmental; D015971:Gene Expression Regulation, Enzymologic; D008969:Molecular Sequence Data; D009841:Oligonucleotides; D016133:Polymerase Chain Reaction; D014176:Protein Biosynthesis; D011817:Rabbits; D014198:Trehalase / Q000737:chemistry / Q000235:genetics*
D017931:DNA Primers; D015342:DNA Probes; D009841:Oligonucleotides; D014198:Trehalase