Transgenic technology: an overview of approaches useful in surgical research.
Advances in transgenic science have created powerful tools for the investigation of both genetic and protein regulatory systems. Recently, transgenic animals have been utilized in several vascular and transplantation research laboratories. The ability to specifically mutate genes important in oncologic and cardiovascular research is leading to a greater understanding of the role of gene and protein regulatory systems in cancer and cardiovascular disease. The expanding use of transgenic animals will undoubtedly increase our insight into complex problems in surgical research. This review briefly describes the various techniques utilized to create transgenic animals including: transgene design, gene-transfer utilizing transfection, microinjection and retroviral infection, as well as the use of embryonic stem cells, and methods for screening transgenic offspring.
Brenin
D R
DR
Department of Surgery, Northwestern University Medical School, Chicago, IL 60611, USA.
Talamonti
M S
MS
Iannaccone
P M
PM
D000818:Animals; D030801:Animals, Genetically Modified / Q000235:genetics / Q000601:surgery*; D004195:Disease Models, Animal; D005818:Genetic Engineering / Q000379:methods*; D005821:Genetic Techniques; D051379:Mice; D008822:Mice, Transgenic / Q000235:genetics / Q000601:surgery; D051381:Rats; D012107:Research Design