Somatostatin in lungfish kidney: an immunohistochemical, autoradiographical and in situ hybridisation study.
The localisation of somatostatin-14 (SST-14) was examined immunohistochemically using the antibody Ab-SST-14 in the kidney of the African lungfish Protopterus annectens. Immunoreactive cells were present in the proximal tubules. In situ hybridisation, using an oligonucleotide probe complementary to mRNA for SST-14 and labeled at the 3'-end with alpha-35S, showed SST-14 mRNA distributed in cells with the same localisation as seen for SST-14 immunoreactive cells. Binding sites for SST-14 were identified with autoradiography using 125I SST-14. Binding sites were concentrated on cells of the proximal tubules. It is suggested that SST-14 may be synthesised in the lungfish mesonephros.
Masini
M A
MA
Institute of Comparative Anatomy, University of Genova, Italy.
Sturla
M
M
Uva
B M
BM
D000818:Animals; D001345:Autoradiography; D002352:Carrier Proteins; D005260:Female; D005399:Fishes / Q000502:physiology*; D019084:Fluorescent Antibody Technique, Indirect; D007106:Immune Sera / Q000276:immunology; D017403:In Situ Hybridization; D007457:Iodine Radioisotopes; D007668:Kidney / Q000033:anatomy & histology / Q000737:chemistry* / Q000378:metabolism; D008297:Male; D012333:RNA, Messenger / Q000032:analysis / Q000235:genetics; D011817:Rabbits; D013004:Somatostatin / Q000032:analysis* / Q000235:genetics / Q000378:metabolism
D002352:Carrier Proteins; D007106:Immune Sera; D007457:Iodine Radioisotopes; D012333:RNA, Messenger; D013004:Somatostatin