Telomerase activity in human leukemic cell lines is inhibited by antisense pentadecadeoxynucleotides targeted against c-myc mRNA.
Telomerase is a ribonucleoprotein complex that is thought to add telomeric repeats to the ends of chromosomes during the replicative phase of the cell cycle. We tested the hypothesis that proto-oncogene c-myc plays an essential role in the regulation of telomerase activity in vertebrate cells by exposing three human leukemic cell lines, HL60, U937, and K562, to 15-mer antisense c-myc oligonucleotides. All the treated cells showed a profound decrease in telomerase activity after c-myc antisense oligomer treatment, whereas cells treated with c-myc sense oligomers showed essentially no change in telomerase activity.
Fujimoto
K
K
Department of Cellular and Molecular Biology, Kanazawa University, Japan.
Takahashi
M
M
D002461:Cell Line, Transformed; D004789:Enzyme Activation / Q000187:drug effects; D018922:HL-60 Cells; D006801:Humans; D004915:Leukemia, Erythroblastic, Acute / Q000201:enzymology / Q000235:genetics; D009838:Oligodeoxyribonucleotides / Q000494:pharmacology*; D016376:Oligonucleotides, Antisense / Q000494:pharmacology*; D000090063:Proto-Oncogene Mas; D016271:Proto-Oncogene Proteins c-myc / Q000235:genetics*; D012333:RNA, Messenger / Q000235:genetics*; D019098:Telomerase / Q000037:antagonists & inhibitors* / Q000378:metabolism; D016212:Transforming Growth Factor beta / Q000494:pharmacology; D014407:Tumor Cells, Cultured; D014409:Tumor Necrosis Factor-alpha / Q000494:pharmacology
C000715470:MAS1 protein, human; D009838:Oligodeoxyribonucleotides; D016376:Oligonucleotides, Antisense; D000090063:Proto-Oncogene Mas; D016271:Proto-Oncogene Proteins c-myc; D012333:RNA, Messenger; D016212:Transforming Growth Factor beta; D014409:Tumor Necrosis Factor-alpha; D019098:Telomerase