Immunohistolocalization of carbonic anhydrase isozymes (CA-I, CA-II and CA-III) in bovine male reproductive tracts.
Immunohistochemical localizations of carbonic anhydrase isozymes (CA-I, CA-II and CA-III) in bovine male reproductive tracts were studied. In bulls, no immunoreaction was seen after treatment with antibodies to CA-I, -II and CA-III in the testis. Specific staining for CA-III, however, was evident in peritubular cells in interstitial tissue of the testis, epididymis. CA-II activity could be detected in epithelium of the epididymis, ductus deferentis and ampulla ductus deferentis. Especially, a strong reaction for CA-II was seen in apical in epithelium of the epididymis in the initial and middle segment. CA-I activity was only founded in ductus deferentis and ampulla ductus deferentis. No or a weak reaction for CA-I, CA-II and CA-III were seen in the three accessory reproductive glands. Specific immunostaining for CA-II and CA-I could be observed in the organ, suggesting the bicarbonate in bovine semen to derive primarily from the genital tract and not accessory reproductive organs. CA-III-positive peritubular cells in interstitial tissue were also stained for alpha smooth muscle actin, and were very similar to contractile myofibroblast cells (Wrobel et al., 1979).
Ichihara
N
N
Department of Animal Models for Human Disease, National Institute of Neuroscience, National Center of Neurology and Psychiatry, Tokyo, Japan.
Sasaki
K
K
Iseri
S
S
Kimura
H
H
Miura
K
K
Nishita
T
T
Asari
M
M
D000185:Actinin / Q000378:metabolism; D000818:Animals; D000918:Antibody Specificity; D002256:Carbonic Anhydrases / Q000378:metabolism*; D002417:Cattle; D003600:Cytosol / Q000201:enzymology*; D005837:Genitalia, Male / Q000201:enzymology*; D007150:Immunohistochemistry; D007527:Isoenzymes / Q000378:metabolism*; D007985:Leydig Cells / Q000201:enzymology; D008297:Male
D007527:Isoenzymes; D000185:Actinin; D002256:Carbonic Anhydrases