Active uptake of serotonin as viability parameter of stored platelets.
The platelet-plasma serotonin (5-HT) exchange in different apheresis products and platelet concentrates (PCs) from buffy coats was studied for quality control over a period of 7 days. In PCs with steadily decreasing pH due to inappropriate gas exchange the platelet 5-HT concentration increases significantly (> 10% positive netto uptake) during the 1st day of storage. Thereafter platelet serotonin drops rapidly with t1/2 = 1 day at pH < 6.5, but platelet counts were constant and 5-HT uptake was inhibited concomitantly. Plasma 5-HT increases as pH decreases. This can be enforced by competitive inhibition of serotonin uptake due to imipramine which also moderates the total 5-HT loss. However, in PC with stable pH the operative 5-HT pump keeps steady-state conditions. The efficacy of the transmembrane uptake mechanism can be adequately monitored by the assessment of the 5-HT platelet/plasma ratio as a new platelet viability parameter.
Benn
H P
HP
Abteilung Transfusionsmedizin und Transplantationsimmunologie, UKE, Universität Hamburg, Germany.
Ganschow
I
I
Fischer
R
R
Dresow
B
B
Cassens
U
U
Dittmer
R
R
Kühnl
P
P
D001792:Blood Platelets / Q000166:cytology / Q000378:metabolism; D001793:Blood Preservation; D002470:Cell Survival; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007962:Leukocytes; D010983:Plateletpheresis; D012701:Serotonin / Q000097:blood*; D013997:Time Factors
D012701:Serotonin