title: Cleavage effect of oligoribonucleotides substituted at the cleavage sites with modified pyrimidine- and purine-nucleosides.
abstract: The precursor of an RNA molecule from T4-infected E. coli cells (p2Spl RNA) has the capacity to cleave itself at specific positions [UpA (139-140) and CpA (170-171)], within a putative loop and stem structure. This sequence-specific cleavage requires at least a monovalent cation and non-ionic detergents. In order to determine the influence of the pyrimidine and purine bases on these sequence-specific cleavage reactions, we studied the cleavage reactions of hairpin loop RNAs substituted at the cleavage sites with modified pyrimidine- and purine-nucleosides. The cleavage was affected by the 2'-hydroxyl groups and the bases of the pyrimidines, and the 6-amino group of the purine.
authors.affiliation: Department of Industrial Chemistry, Chiba Institute of Technology, Narashino, Japan.
authors.forename: K
authors.initials: K
authors.lastname: Hosono
authors.forename: H
authors.initials: H
authors.lastname: Gozu
authors.forename: H
authors.initials: H
authors.lastname: Hosaka
authors.forename: K
authors.initials: K
authors.lastname: Sakamoto
authors.forename: S
authors.initials: S
authors.lastname: Yokoyama
authors.forename: K
authors.initials: K
authors.lastname: Takai
authors.forename: H
authors.initials: H
authors.lastname: Takaku
mesh_terms: D001482:Base Composition; D001483:Base Sequence; D001665:Binding Sites / Q000235:genetics; D009690:Nucleic Acid Conformation; D009843:Oligoribonucleotides / Q000138:chemical synthesis / Q000737:chemistry* / Q000378:metabolism; D011684:Purine Nucleosides / Q000138:chemical synthesis / Q000737:chemistry* / Q000378:metabolism; D011741:Pyrimidine Nucleosides / Q000138:chemical synthesis / Q000737:chemistry* / Q000378:metabolism; D016337:RNA, Catalytic / Q000737:chemistry / Q000235:genetics / Q000378:metabolism
chemical_list: D009843:Oligoribonucleotides; D011684:Purine Nucleosides; D011741:Pyrimidine Nucleosides; D016337:RNA, Catalytic