Barrier function of reconstructed epidermis at the air-liquid interface: influence of dermal cells and extracellular components.
To evaluate the epidermal barrier function of in vitro reconstructed epidermis, we measured the penetration of estradiol and water across human keratinocytes cultured in defined medium (DM), in the presence of proliferative fibroblasts (pF) or conditioned medium derived from pF, at the air-liquid interface on synthetic porous membrane, noncoated or coated with laminin, fibronectin, type I collagen or type IV collagen. Ultrastructural analysis showed a well-developed stratum corneum whatever the culture conditions. The permeability of reconstructed epidermis in DM on a noncoated porous membrane was 5- to 10-fold higher than human native epidermis, with both tracers. No significant change in barrier function was observed whatever the culture conditions.
Robert
M
M
Laboratoire de Pharmacologie, Unité de Recherches en Dermopharmacologie, Faculté de Pharmacie, Châtenay-Malabry, France.
Dusser
I
I
Muriel
M P
MP
Noël-Hudson
M S
MS
Aubery
M
M
Wepierre
J
J
D002454:Cell Differentiation / Q000502:physiology; D002455:Cell Division / Q000502:physiology; D002463:Cell Membrane Permeability; D002478:Cells, Cultured; D002675:Child, Preschool; D017077:Culture Media, Conditioned; D000078404:Epidermal Cells; D004817:Epidermis / Q000502:physiology*; D004958:Estradiol / Q000493:pharmacokinetics; D005109:Extracellular Matrix / Q000502:physiology*; D005347:Fibroblasts / Q000166:cytology / Q000502:physiology; D006801:Humans; D015603:Keratinocytes / Q000166:cytology / Q000502:physiology* / Q000648:ultrastructure; D008297:Male; D008567:Membranes, Artificial; D012869:Skin Absorption / Q000502:physiology; D012879:Skin Physiological Phenomena; D014867:Water / Q000378:metabolism
D017077:Culture Media, Conditioned; D008567:Membranes, Artificial; D014867:Water; D004958:Estradiol