title: Elevated expression of proteoglycans in proliferating vascular smooth muscle cells.
abstract: Smooth muscle cell (SMC) proliferation and increased production of arterial wall proteoglycans (PG) are implicated in atherogenesis. We investigated the effect of SMC proliferation on the biosynthesis of PG and the ability of the newly synthesized PG to bind low density lipoprotein (LDL). Proliferating and quiescent human aortic SMC were pulsed with [35S]sulfate for 24 h. Secreted and cell-associated PG were then analyzed. When SMC plated at a low density were induced to proliferate, PG synthesis increased significantly in comparison with quiescent cells. This was the net result of a 2.7-fold increase in secreted PG and a 1.3-fold increase in cell-associated PG. The increased PG synthesis in proliferating SMC correlated with a significant increase in the steady-state level of mRNA for perlecan and biglycan, and a modest increase in the versican-specific mRNA. The mRNA for decorin showed a 40% decrease. The increased PG secretion in proliferating cultures was due to increases in heparan sulfate PG, dermatan sulfate PG, and chondroitin sulfate PG secretion. Quiescent SMC at confluency produced 50% less PG than the corresponding SMC plated at a low density. Although confluent SMC stimulated to proliferate also had increased PG synthesis, this was 50% less than the PG synthesis by proliferating SMC that were initially plated at a low density. The PG synthesized by proliferating and quiescent SMC did not differ in charge density and molecular size. Secreted PG from both quiescent and proliferating cultures contained subfractions that bound LDL with high affinity. However, compared with quiescent cultures, the proliferating cultures produced more of a PG subfraction that exhibited very high affinity to LDL (31.6% in quiescent cultures versus 40.8% in proliferating cultures). These results indicate that PG metabolism is altered significantly in proliferating human SMC which might have implications in the pathophysiology of atherosclerosis.
authors.affiliation: Cardiac Transplantation Unit, Ochsner Medical Institute, New Orleans, LA 70121, USA.
authors.forename: Z
authors.initials: Z
authors.lastname: Tao
authors.forename: F W
authors.initials: FW
authors.lastname: Smart
authors.forename: J E
authors.initials: JE
authors.lastname: Figueroa
authors.forename: D L
authors.initials: DL
authors.lastname: Glancy
authors.forename: P
authors.initials: P
authors.lastname: Vijayagopal
mesh_terms: D001013:Aorta, Thoracic; D002455:Cell Division; D002478:Cells, Cultured; D002846:Chromatography, Affinity; D006801:Humans; D007399:Interphase; D009131:Muscle, Smooth, Vascular / Q000166:cytology / Q000378:metabolism*; D011509:Proteoglycans / Q000096:biosynthesis* / Q000235:genetics / Q000378:metabolism; D012333:RNA, Messenger / Q000032:analysis
chemical_list: D011509:Proteoglycans; D012333:RNA, Messenger