Microinjection of cre recombinase RNA induces site-specific recombination of a transgene in mouse oocytes.
We have developed a strategy for producing single copy transgenic mouse lines using Cre-loxP site specific recombination. The method is based on transient expression of the recombinase after injection of in vitro transcribed mRNA into the cytoplasm of fertilised eggs containing multiple copies of the transgene. The success rate of the recombination event is 100% (15 out of 15).
de Wit
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Erasmus University Rotterdam, MGC-Department of Cell Biology, PO Box 1738, 3000 DR Rotterdam, The Netherlands.
Drabek
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Grosveld
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D000818:Animals; D003001:Cloning, Molecular; D003593:Cytoplasm / Q000378:metabolism; D005260:Female; D015870:Gene Expression; D019426:Integrases / Q000235:genetics*; D008297:Male; D051379:Mice; D008822:Mice, Transgenic; D008845:Microinjections; D009865:Oocytes / Q000648:ultrastructure*; D010849:Picornaviridae / Q000235:genetics; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000008:administration & dosage*; D011995:Recombination, Genetic; D019076:Transgenes / Q000235:genetics*; D014764:Viral Proteins
D012333:RNA, Messenger; D014764:Viral Proteins; C045073:Cre recombinase; D019426:Integrases