Mouse latent TGF-beta binding protein-2: molecular cloning and developmental expression.
The molecular cloning and developmental expression of mouse LTBP-2 are presented here. We established the identity of the cDNA by sequence comparison (80% identity with human LTBP-2) and by chromosome localization (mouse chromosome 12, band D, a region of conserved synteny with the human LTBP-2 gene). In contrast to LTBP-1 and LTBP-3, mouse LTBP-2 apparently is a more modular protein, with proline/glycine-rich sequences always alternating with clusters of cysteine-rich structural motifs. We found for the first time that LTBP-2 gene expression in mouse embryos was restricted to cartilage perichondrium and blood vessels, a somewhat surprising result since other LTBP genes are widely expressed in rodent tissues. Therefore, mouse LTBP-2 may play a critical role in the assembly of latent TGF-beta complexes in developing elastic tissues such as cartilage and blood vessel.
Fang
J
J
Department of Pathology, University of Michigan Medical School, Ann Arbor 48109-0417, USA.
Li
X
X
Smiley
E
E
Francke
U
U
Mecham
R P
RP
Bonadio
J
J
D016475:3T3 Cells; D048868:Adaptor Proteins, Signal Transducing; D000595:Amino Acid Sequence; D000818:Animals; D002352:Carrier Proteins / Q000096:biosynthesis* / Q000737:chemistry / Q000235:genetics* / Q000302:isolation & purification; D002356:Cartilage / Q000378:metabolism; D002874:Chromosome Mapping; D004622:Embryo, Mammalian / Q000378:metabolism; D018507:Gene Expression Regulation, Developmental; D006801:Humans; D053779:Latent TGF-beta Binding Proteins; D051379:Mice; D008815:Mice, Inbred Strains; D008969:Molecular Sequence Data; D009131:Muscle, Smooth, Vascular / Q000378:metabolism; D010455:Peptides / Q000737:chemistry / Q000235:genetics; D014176:Protein Biosynthesis; D016212:Transforming Growth Factor beta / Q000378:metabolism*
D048868:Adaptor Proteins, Signal Transducing; D002352:Carrier Proteins; C509032:LTBP1 protein, human; C509035:LTBP2 protein, human; C509038:LTBP3 protein, human; D053779:Latent TGF-beta Binding Proteins; C509033:Ltbp1 protein, mouse; C509036:Ltbp2 protein, mouse; C509039:Ltbp3 protein, mouse; D010455:Peptides; D016212:Transforming Growth Factor beta