title: Regulation of neuronal specification in the zebrafish spinal cord by Delta function.
abstract: The vertebrate spinal cord consists of a large number of different cell types in close proximity to one another. The identities of these cells appear to be specified largely by information acquired from their local environments. We report here that local cell-cell interactions, mediated by zebrafish homologues of the Drosophila melanogaster neurogenic gene, Delta, regulate specification of diverse neuronal types in the ventral spinal cord. We describe identification of a novel zebrafish Delta gene expressed specifically in the nervous system and show, by expressing a dominant negative form of Delta protein in embryos, that Delta proteins mediate lateral inhibition in the zebrafish spinal cord. Furthermore, we find that Delta function is important for specification of a variety of spinal cord neurons, suggesting that lateral inhibition serves to diversify neuronal fate during development of the vertebrate spinal cord.
authors.affiliation: Institute of Neuroscience, University of Oregon, Eugene 97403, USA. appel@uoneuro.uoregon.edu
authors.forename: B
authors.initials: B
authors.lastname: Appel
authors.forename: J S
authors.initials: JS
authors.lastname: Eisen
mesh_terms: D000818:Animals; D000954:Antigens, Surface / Q000235:genetics; D019521:Body Patterning / Q000235:genetics; D003001:Cloning, Molecular; D051959:ELAV Proteins; D000067778:ELAV-Like Protein 3; D004627:Embryonic Induction; D018507:Gene Expression Regulation, Developmental / Q000502:physiology*; D007395:Interneurons / Q000166:cytology; D047908:Intracellular Signaling Peptides and Proteins; D008565:Membrane Proteins / Q000235:genetics / Q000502:physiology*; D008969:Molecular Sequence Data; D009046:Motor Neurons / Q000166:cytology; D009419:Nerve Tissue Proteins / Q000235:genetics; D009474:Neurons / Q000166:cytology*; D012333:RNA, Messenger / Q000032:analysis / Q000494:pharmacology; D016601:RNA-Binding Proteins; D013116:Spinal Cord / Q000737:chemistry / Q000166:cytology / Q000196:embryology*; D015027:Zebrafish; D029961:Zebrafish Proteins
chemical_list: D000954:Antigens, Surface; D051959:ELAV Proteins; D000067778:ELAV-Like Protein 3; D047908:Intracellular Signaling Peptides and Proteins; D008565:Membrane Proteins; D009419:Nerve Tissue Proteins; D012333:RNA, Messenger; D016601:RNA-Binding Proteins; D029961:Zebrafish Proteins; C064045:delta protein; C088635:elavl3 protein, zebrafish