title: Type VI microfilaments interact with a specific region of banded collagen fibrils in skin.
abstract: Immunolocalization studies demonstrate that Type VI collagen forms a flexible network that interweaves among collagen fibrils in the dermis of skin as well as in other loose connective tissues. Although binding of Type VI collagen with other matrix components has been suggested, no structural evidence supporting these studies has been reported. In this study, we demonstrate that Type VI microfilaments consistently crossbanded collagen fibrils near the "d" band, indicating that the interaction of Type VI collagen with banded fibrils is not passive. This "d" band is also the location of the binding domain of decorin to banded fibrils, suggesting that decorin mediates the interaction of Type VI microfilaments with banded fibers. Examination of the architecture of the Type VI network in a decorin nullizygous mouse demonstrates a continuance of this specific interaction, indicating that the association is not entirely dependent on the presence of decorin. At least one other component, whose identity is uncertain, persists near the "d" band, which may also serve to mediate the attachment of Type VI collagen to collagen fibrils.
authors.affiliation: Shriners Hospital for Children, Portland, Oregon 97201, USA.
authors.forename: D R
authors.initials: DR
authors.lastname: Keene
authors.forename: C C
authors.initials: CC
authors.lastname: Ridgway
authors.forename: R V
authors.initials: RV
authors.lastname: Iozzo
mesh_terms: D008841:Actin Cytoskeleton / Q000737:chemistry* / Q000648:ultrastructure*; D000818:Animals; D001665:Binding Sites; D003094:Collagen / Q000032:analysis*; D058575:Decorin; D016326:Extracellular Matrix Proteins; D006801:Humans; D007150:Immunohistochemistry; D051379:Mice; D016253:Microscopy, Immunoelectron; D011509:Proteoglycans / Q000032:analysis; D012867:Skin / Q000737:chemistry / Q000648:ultrastructure*
chemical_list: C546878:DCN protein, human; C546879:Dcn protein, mouse; D058575:Decorin; D016326:Extracellular Matrix Proteins; D011509:Proteoglycans; D003094:Collagen