IRS-PCR-based genetic mapping of the huntingtin interacting protein gene (HIP1) on mouse chromosome 5.
Huntington's disease (HD) is a devastating central nervous system disorder. Even though the gene responsible has been positionally cloned recently, its etiology has remained largely unclear. To investigate potential disease mechanisms, we conducted a search for binding partners of the HD-protein huntingtin. With the yeast two-hybrid system, one such interacting factor, the huntingtin interacting protein-1 (HIP-1), was identified (Wanker et al. 1997; Kalchman et al. 1997) and the human gene mapped to 7q11.2. In this paper we demonstrate the localization of the HIP1 mouse homologue (Hip1) into a previously identified region of human-mouse synteny on distal mouse Chromosome (Chr) 5, both employing an IRS-PCR-based mapping strategy and traditional fluorescent in situ hybridization (FISH) mapping.
Himmelbauer
H
H
Max-Planck-Institute for Molecular Genetics, Berlin-Dahlem, Germany.
Wedemeyer
N
N
Haaf
T
T
Wanker
E E
EE
Schalkwyk
L C
LC
Lehrach
H
H
D000818:Animals; D002874:Chromosome Mapping; D003001:Cloning, Molecular; D003360:Cosmids; D003433:Crosses, Genetic; D004268:DNA-Binding Proteins; D016678:Genome; D017404:In Situ Hybridization, Fluorescence; D051379:Mice; D009419:Nerve Tissue Proteins / Q000235:genetics*; D016133:Polymerase Chain Reaction / Q000379:methods*; D011110:Polymorphism, Genetic
D004268:DNA-Binding Proteins; C073368:HIP1 protein, human; C490758:Hap1 protein, mouse; D009419:Nerve Tissue Proteins