Fluorometric determination of serum and urinary aluminium with 8-quinolinol by kinetic-differentiation-mode micellar chromatography.
A new fluorometric method has been developed for the determination of aluminium with 8-quinolinol by kinetic-differentiation-mode micellar chromatography. The proposed method enabled the determination of aluminium down to 1 microgram/l in human serum and urine without preliminary deproteinization. The most remarkable point of this method is that only aluminium ion selectively responds among metal ions. The complex formation of aluminium in serum with 8-quinolinol was completed within a few minutes at room temperature. The serum matrix and aluminium chelate were separated on a reversed-phase column with an eluent containing acetonitrile, sodium dodecylsulfate, and Triton X-100, and aluminium chelate was detected at Ex 370 nm, Em 504 nm. The values obtained by this method were in good agreement with those of Zeeman graphite-furnace atomic absorption spectrometry. The proposed method will provide a simple and rapid technique for the determination aluminium in medical fields.
Sato
M
M
Department of Applied Chemistry, Graduate School of Engineering, Tohoku University, Sendai, Japan.
Yoshimura
H
H
Shimmura
T
T
Obi
H
H
Hatakeyama
S
S
Kaneko
E
E
Hoshino
H
H
Yotsuyanagi
T
T
D000535:Aluminum / Q000032:analysis* / Q000097:blood / Q000652:urine; D002853:Chromatography, Liquid / Q000379:methods*; D006801:Humans; D007202:Indicators and Reagents / Q000737:chemistry; D007700:Kinetics; D008670:Metals / Q000737:chemistry; D008823:Micelles; D015125:Oxyquinoline / Q000737:chemistry*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D012967:Sodium Dodecyl Sulfate / Q000737:chemistry; D013050:Spectrometry, Fluorescence; D013054:Spectrophotometry, Atomic
D007202:Indicators and Reagents; D008670:Metals; D008823:Micelles; D012967:Sodium Dodecyl Sulfate; D015125:Oxyquinoline; D000535:Aluminum