Use of SSU rDNA group-I intron to distinguish Monilinia fructicola from M. laxa and M. fructigena.
Monilinia fructicola, M. laxa and M. fructigena are the causal agents of brown rot of pome and stone fruits. M. fructicola is not present in Europe and is classed as a quarantine pathogen in EU countries. A 418-bp group-I intron has been located in the small subunit (SSU) rDNA gene of M. fructicola which is absent from M. laxa and M. fructigena. PCR primers specific to the 3'-region of the intron together with the SSU rDNA primer NS5 were able to amplify a 444-bp product from M. fructicola and fruit tissue infected with M. fructicola but not from the other two species. This allows for the rapid and sensitive detection of this pathogen in planta.
Fulton
C E
CE
Department of Applied Plant Science, Queen's University of Belfast, UK. c.fulton@al.qub.ac.uk
Brown
A E
AE
D001203:Ascomycota / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D001483:Base Sequence; D004271:DNA, Fungal / Q000032:analysis*; D004275:DNA, Ribosomal / Q000032:analysis*; D005638:Fruit / Q000382:microbiology; D005800:Genes, Fungal; D007438:Introns / Q000235:genetics; D008969:Molecular Sequence Data; D010935:Plant Diseases; D016133:Polymerase Chain Reaction; D012331:RNA, Fungal / Q000235:genetics*; D012337:RNA, Ribosomal, 18S / Q000235:genetics*; D013045:Species Specificity
D004271:DNA, Fungal; D004275:DNA, Ribosomal; D012331:RNA, Fungal; D012337:RNA, Ribosomal, 18S