Bioactivities and secondary structure of mast cell degranulating (MCD) peptide analogs.
Three analogs of Mast Cell Degranulating (MCD) peptide with C-terminal and one analog with N- and C-terminal deletions were synthesized and assayed for histamine-releasing activity in mast cells. Des(20-22)-MCD and des(21)-MCD markedly decreased this activity. In des(16-17,21)-MCD this activity was completely abolished. By contrast, when the C- and N-termini were truncated in des(1-2,20-22)-MCD, the full activity of MCD peptide was restored. The possibility that these analogs trigger or inhibit oxygen radicals from neutrophils was examined in a cell-free system with a chemiluminescence assay. None of the above analogs exhibited inflammatory or anti-inflammatory activity. Changes in biological activities were correlated with structural changes, as seen by circular dichroism (CD) spectroscopy.
Buku
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Department of Physiology and Biophysics, Mount Sinai School of Medicine, New York, NY 10029, USA.
Maulik
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Hook
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D000595:Amino Acid Sequence; D000818:Animals; D002942:Circular Dichroism; D006636:Histamine Release / Q000187:drug effects*; D008163:Luminescent Measurements; D008297:Male; D008407:Mast Cells / Q000187:drug effects*; D008969:Molecular Sequence Data; D010455:Peptides / Q000737:chemistry* / Q000494:pharmacology*; D017433:Protein Structure, Secondary; D051381:Rats; D017207:Rats, Sprague-Dawley; D017382:Reactive Oxygen Species / Q000378:metabolism; D017384:Sequence Deletion
D010455:Peptides; D017382:Reactive Oxygen Species; C008331:mast cell degranulating peptide