Granulocyte activation by Helicobacter pylori.
Helicobacter pylori-associated gastritis (HAG) is characterized by granulocytic and mononuclear cell infiltrates within infected gastric mucosa. Since the bacterium does not invade the epithelial layer, it must be assumed that components or products of the pathogen which permeate the epithelial barrier may initiate chemotaxis and activation of neutrophils. The aim of this study was to evaluate the effect of H. pylori water soluble protein (WSP) components on the induction of granulocyte adherence and activation. The results show that H. pylori WSP led to enhanced expression of the beta 2-integrin CD11b/CD18 on the granulocyte surface. Following upregulation of this adhesion molecule, activated granulocytes demonstrated increased adhesion to human endothelial cells (HUVEC) in culture. These observations support the hypothesis that in vivo neutrophil activation may be a direct result of H. pylori constituents promoting transendothelial migration into the lamina propria of infected gastric mucosa.
Hatz
R A
RA
Department of Surgery, Klinikum Grosshadern, Ludwig-Maximilians-University Munich.
Meimarakis
G
G
Lehn
N
N
Bayerdörffer
E
E
Von Jan
N
N
Enders
G
G
D001426:Bacterial Proteins / Q000502:physiology; D018821:CD18 Antigens / Q000096:biosynthesis; D002448:Cell Adhesion; D002478:Cells, Cultured; D002634:Chemotaxis, Leukocyte; D004730:Endothelium, Vascular / Q000382:microbiology / Q000502:physiology*; D005434:Flow Cytometry / Q000379:methods; D006098:Granulocytes / Q000382:microbiology / Q000502:physiology*; D016480:Helicobacter pylori / Q000502:physiology*; D006801:Humans; D016177:Macrophage-1 Antigen / Q000096:biosynthesis; D008954:Models, Biological; D014471:Umbilical Veins
D001426:Bacterial Proteins; D018821:CD18 Antigens; D016177:Macrophage-1 Antigen