Use of Rotofor preparative isoelectrofocusing cell in protein purification procedure.
The Rotofor cell is a preparative isoelectric focusing (IEF) apparatus, in which IEF is performed entirely in free solution. Electrofocusing in Rotofor cell has been described as well-suited for use at any stage of a purification scheme. However, it has some important limitations in resolving complex mixtures of proteins. This paper describes the advantages and disadvantages of using the Rotofor cell in purification protocols.
Ayala
A
A
Departamento de Bioquimica, Bromatologia y Toxicologia, Facultad de Farmacia, Universidad de Sevilla, Spain.
Parrado
J
J
Machado
A
A
D000818:Animals; D001923:Brain Chemistry; D004591:Electrophoresis, Polyacrylamide Gel; D007525:Isoelectric Focusing / Q000295:instrumentation; D008970:Molecular Weight; D010734:Phosphogluconate Dehydrogenase / Q000302:isolation & purification; D011506:Proteins / Q000302:isolation & purification*; D051381:Rats; D013482:Superoxide Dismutase / Q000302:isolation & purification
D011506:Proteins; D010734:Phosphogluconate Dehydrogenase; D013482:Superoxide Dismutase