title: [Effect of lignin preparations and cultivation conditions on the ligninolytic complex of the fungus Pleurotus floridae, the wood white-rot pathogen].
abstract: Effect of various cultivation conditions and lignin preparations on the enzymes of ligninolytic enzyme complex of white-rot fungus Pleurotus floridae has been studied. The maximal Mn-peroxidase activity was revealed in the medium with low nitrogen content (1.2 mM); maximal values of cellobiose quinone oxidoreductase activity were observed in the media with high nitrogen content (7.2 mM); maximal values of laccase activity in the media with low content of glucose (2 g/l) during Pleurotus floridae cultivation in Kirk's stationary cultures have been shown. Employment of submerged cultivation under conditions of mycelium immobilization on polyurethane carriers allowed us to increase laccase activity twice as compared with cultivation in small stationary cultures, while had the crucial effect on the Mn-peroxidase activity. The selective effect of the studied lignin preparations on the components of ligninolytic complex and their isoenzymes has been stated. The dependence of laccase and Mn-peroxidase activities on high and low-molecular weight fractions balance in lignin preparations has been established.
authors.forename: E N
authors.initials: EN
authors.lastname: Dombrovskaia
authors.forename: S S
authors.initials: SS
authors.lastname: Kostyshin
mesh_terms: D002237:Carbohydrate Dehydrogenases / Q000187:drug effects / Q000378:metabolism; D003470:Culture Media; D006868:Hydrolysis; D008031:Lignin / Q000494:pharmacology*; D008970:Molecular Weight; D010544:Peroxidases / Q000187:drug effects / Q000378:metabolism; D011124:Polyporaceae / Q000187:drug effects* / Q000201:enzymology
chemical_list: D003470:Culture Media; D008031:Lignin; D002237:Carbohydrate Dehydrogenases; C019859:cellobiose-quinone oxidoreductase; D010544:Peroxidases; C051129:manganese peroxidase
vernacular_title: Vliianie preparatov lignina i usloviĭ kyl'tivirovaniia na ligninoliticheskiĭ kompleks griba Pleurotus floridae-- vozbuditelia beloĭ gnili drevesiny.