title: Spectrum of cytokine gene expression in intestinal mucosal lesions of Crohn's disease and ulcerative colitis.
abstract: OBJECTIVE
  We investigated the mRNA expression of spectrum of cytokines in the colonic mucosa in inflammatory bowel disease (IBD).
  
  
  METHODS
  The expression of cytokine gene was evaluated by using the reverse transcription-polymerase chain reaction and the radioactivity of amplified cDNA standardized by coamplified beta-actin cDNA.
  
  
  RESULTS
  Crohn's disease and ulcerative colitis showed significantly increased expression of IL-1beta, IL-6, IL-8 and TNF-alpha mRNA as compared with controls (p < 0.05).
  
  
  CONCLUSIONS
  Proinflammatory cytokines such as IL-1beta, IL-6, IL-8 and TNF-alpha are closely involved in the immune abnormalities of inflammatory mucosal lesions in IBD.
authors.affiliation: Third Department of Internal Medicine, Niigata University School of Medicine, Japan.
authors.forename: K
authors.initials: K
authors.lastname: Funakoshi
authors.forename: K
authors.initials: K
authors.lastname: Sugimura
authors.forename: K
authors.initials: K
authors.lastname: Anezaki
authors.forename: H
authors.initials: H
authors.lastname: Bannai
authors.forename: K
authors.initials: K
authors.lastname: Ishizuka
authors.forename: H
authors.initials: H
authors.lastname: Asakura
mesh_terms: D000293:Adolescent; D000328:Adult; D003093:Colitis, Ulcerative / Q000235:genetics / Q000276:immunology*; D003424:Crohn Disease / Q000235:genetics / Q000276:immunology*; D016207:Cytokines / Q000096:biosynthesis* / Q000235:genetics / Q000276:immunology; D005260:Female; D005770:Gastrointestinal Neoplasms / Q000235:genetics / Q000276:immunology*; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D008297:Male; D008875:Middle Aged; D016133:Polymerase Chain Reaction / Q000379:methods; D012333:RNA, Messenger / Q000378:metabolism; D012334:RNA, Neoplasm / Q000378:metabolism; D014158:Transcription, Genetic; D015854:Up-Regulation
chemical_list: D016207:Cytokines; D012333:RNA, Messenger; D012334:RNA, Neoplasm