[Neuronal differentiation of human small cell lung cancer cell line PC-6 by Solcoseryl].
Solcoseryl is composed of extracts from calf blood, and is a drug known to activate tissue respiration. In the present study, I demonstrated the cell biological effects of Solcoseryl on a human small cell lung cancer cell line, PC-6, by analyzing cell morphology, cell growth, expression of neuronal differentiation markers, and the ras proto-oncogene product(ras p21). Exposure of PC-6 cells to Solcoseryl at the concentration of 200 microliters/ml induced (1) cell morphological changes, including neurodendrite-like projections from the cell surface, and (2) complete inhibition of cell growth, that was shown by the loss of Ki-67 expression. Solcoseryl also induced the expression of neurofilament protein and acetylcholinesterase, both of which are markers of neuronal differentiation. Moreover, it upregulated the expression of the ras proto-oncogene product, ras p21. Taken together, these data suggest that Solcoseryl is composed of component(s) which can induce neuronal differentiation of the human small cell lung cancer cell line, PC-6.
Shimizu
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First Department of Internal Medecine, Hokkaido University School of Medicine, Sapporo, Japan.
D000110:Acetylcholinesterase / Q000378:metabolism; D000183:Actihaemyl / Q000494:pharmacology*; D014408:Biomarkers, Tumor / Q000378:metabolism; D018288:Carcinoma, Small Cell / Q000378:metabolism / Q000473:pathology*; D002454:Cell Differentiation / Q000187:drug effects; D002455:Cell Division / Q000187:drug effects; D003712:Dendrites / Q000473:pathology; D006801:Humans; D008175:Lung Neoplasms / Q000378:metabolism / Q000473:pathology*; D016900:Neurofilament Proteins / Q000378:metabolism; D000090063:Proto-Oncogene Mas; D016283:Proto-Oncogene Proteins p21(ras) / Q000378:metabolism; D014407:Tumor Cells, Cultured
D014408:Biomarkers, Tumor; C000715470:MAS1 protein, human; D016900:Neurofilament Proteins; D000090063:Proto-Oncogene Mas; D000183:Actihaemyl; D000110:Acetylcholinesterase; C501009:HRAS protein, human; D016283:Proto-Oncogene Proteins p21(ras)