title: Adult insect mushroom body neurons in primary culture: cell morphology and characterization of potassium channels.
abstract: The dissociation and maintenance in culture of cells derived from the mushroom bodies of adult crickets (Acheta domesticus) are described. This primary culture was developed in order to investigate maturation and differentiation of mushroom-body cells including Kenyon cells, the major intrinsic interneurons of mushroom bodies, which have been shown to be involved in learning and memory in insects. Three distinct cell types were observed, all identified as neural cells on the basis of their size, morphology and immunocytochemical staining with horseradish peroxidase. These cells appear to correspond to the three cell types observed in vivo: Kenyon cells, ganglion mother cells and neuroblasts. Some cells showed neurite growth, usually with long unipolar processes, occasionally with either bipolar or, more rarely, multipolar processes. Neuronal cell bodies readily formed seals with patch pipettes, allowing stable, whole-cell, patch-clamp electrophysiological recordings. Depolarization of the cell under voltage-clamp resulted in at least two types of outwardly directed potassium currents: a delayed rectifier-type of current that was sensitive to tetraethylammonium, and a cadmium-sensitive current with rapid inactivation. Neither type of current was affected by quinidine, a blocker of potassium currents recorded from pupal honeybee Kenyon cells. Other ionic currents, which have yet to be characterized, were also observed.
authors.affiliation: CNRS, Laboratoire de Neurobiologie, Neuroendocrinologie des Insectes, 31, chemin Joseph Aiguier, 13402 Marseille cedex 20, France.
authors.forename: M
authors.initials: M
authors.lastname: Cayre
authors.forename: S D
authors.initials: SD
authors.lastname: Buckingham
authors.forename: A
authors.initials: A
authors.lastname: Strambi
authors.forename: C
authors.initials: C
authors.lastname: Strambi
authors.forename: D B
authors.initials: DB
authors.lastname: Sattelle
mesh_terms: D000818:Animals; D001370:Axonal Transport; D001921:Brain / Q000166:cytology / Q000502:physiology; D002104:Cadmium / Q000494:pharmacology; D002470:Cell Survival; D002478:Cells, Cultured; D046508:Culture Techniques / Q000379:methods; D004261:DNA Replication; D017952:Ganglia, Invertebrate / Q000166:cytology / Q000502:physiology; D006135:Gryllidae; D006735:Horseradish Peroxidase; D008564:Membrane Potentials / Q000187:drug effects; D009474:Neurons / Q000166:cytology* / Q000502:physiology*; D015221:Potassium Channels / Q000187:drug effects / Q000502:physiology*; D011802:Quinidine / Q000494:pharmacology; D019789:Tetraethylammonium / Q000494:pharmacology
chemical_list: D015221:Potassium Channels; D002104:Cadmium; D019789:Tetraethylammonium; D006735:Horseradish Peroxidase; D011802:Quinidine