Evaluation of Parvo B19, CMV and HPV viruses in human aborted material using the polymerase chain reaction technique.
OBJECTIVE
To investigate the role of human parvovirus B19 (Parvo B19), cytomegalovirus (CMV) and human papilloma virus (HPV) viruses in the aetiopathogenesis of spontaneous abortions.


METHODS
Abortion material from 102 cases of women with spontaneous abortions were analysed for the presence of Parvo B19, CMV and HPV DNA using the polymerase chain reaction (PCR) technique. Serological assays were used for the detection of specific IgM and IgG antibodies against Parvo B19 virus and CMV in the maternal sera.


RESULTS
Parvo B19 virus genome was detected in two cases of spontaneous abortion, by PCR amplification, while CMV and HPV genomes were not observed. Serological markers were indicative for Parvo B19 virus and CMV infection in ten and four cases, respectively.


CONCLUSIONS
PCR is a useful method for investigating the viral contribution to the aetiopathogenesis of spontaneous abortions and for detecting the viral genome in the abortion material. This study of 102 cases of spontaneous abortion does not implicate CMV and HPV in the aetiopathogenesis of spontaneous abortion, although it indicates a possible abortional role for Parvo B19 virus.
Sifakis
S
S
Department of Obstetrics and Gynecology, University Hospital, Heraklion, Crete, Greece.
Ergazaki
M
M
Sourvinos
G
G
Koffa
M
M
Koumantakis
E
E
Spandidos
D A
DA
D000022:Abortion, Spontaneous / Q000821:virology*; D000914:Antibodies, Viral / Q000097:blood; D003587:Cytomegalovirus / Q000235:genetics* / Q000276:immunology; D004279:DNA, Viral / Q000032:analysis*; D005260:Female; D005865:Gestational Age; D006801:Humans; D007074:Immunoglobulin G / Q000097:blood; D007075:Immunoglobulin M / Q000097:blood; D027383:Papillomaviridae / Q000235:genetics*; D010322:Parvoviridae Infections / Q000821:virology; D016732:Parvovirus B19, Human / Q000235:genetics* / Q000276:immunology; D016133:Polymerase Chain Reaction; D011247:Pregnancy; D014777:Virus Diseases / Q000821:virology*
D000914:Antibodies, Viral; D004279:DNA, Viral; D007074:Immunoglobulin G; D007075:Immunoglobulin M