title: Reduced blood accumulation of biotinylated monoclonal antibody A7 after the subsequent administration of avidin.
abstract: Clear immunoscintigraphy with radiolabeled monoclonal antibodies (MAbs) requires a high tumor tissue/blood ratio of radioactivity. In this study, we attempted to obtain a high tumor tissue/blood ratio by the active removal of radiolabeled MAb from the circulation, using the avidin-biotin system. Biotinylated 125I-labeled MAb A7 was injected intravenously into nude mice bearing a human colon cancer (WiDr) xenograft. Avidin was injected 24 h later. The tumor tissue/blood ratio of radioactivity was almost four times that of controls. These results suggest that biotinylated 125I-labeled MAb A7 and avidin are potentially useful for the rapid immunodetection of human colon cancer.
authors.affiliation: The First Department of Surgery, Kyoto Prefectural University of Medicine, Japan.
authors.forename: H
authors.initials: H
authors.lastname: Nishi
authors.forename: T
authors.initials: T
authors.lastname: Yamaguchi
authors.forename: E
authors.initials: E
authors.lastname: Otsuji
authors.forename: T
authors.initials: T
authors.lastname: Kotani
authors.forename: K
authors.initials: K
authors.lastname: Taniguchi
authors.forename: K
authors.initials: K
authors.lastname: Okamoto
authors.forename: Y
authors.initials: Y
authors.lastname: Yata
authors.forename: H
authors.initials: H
authors.lastname: Tsuruta
authors.forename: T
authors.initials: T
authors.lastname: Takahashi
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000493:pharmacokinetics*; D000951:Antigens, Neoplasm / Q000276:immunology; D001360:Avidin / Q000008:administration & dosage* / Q000494:pharmacology; D001710:Biotin / Q000097:blood / Q000493:pharmacokinetics*; D003110:Colonic Neoplasms / Q000000981:diagnostic imaging* / Q000276:immunology; D006801:Humans; D007668:Kidney / Q000378:metabolism; D008099:Liver / Q000378:metabolism; D051379:Mice; D008819:Mice, Nude; D009368:Neoplasm Transplantation; D011863:Radioimmunoassay; D011877:Radionuclide Imaging; D013154:Spleen / Q000378:metabolism; D013997:Time Factors; D014407:Tumor Cells, Cultured
chemical_list: D000911:Antibodies, Monoclonal; D000951:Antigens, Neoplasm; D001360:Avidin; D001710:Biotin