Step-gradient capillary electrochromatography.
The analytical benefits of using a step-gradient in capillary electrochromatography (CEC) are demonstrated. The application of step-gradient CEC to the analysis of six diuretics of widely differing lipophilicities was evaluated and shown to result in a marked reduction in the analysis time and an improvement in the peak shape for later-eluting lipophilic components. When the step-gradient approach was performed in an automated mode, the retention time RSD for repeated injections was below 1%.
Euerby
M R
MR
Pharmaceutical and Analytical R & D, Astra Charnwood, Loughborough, Leicestershire, UK.
Gilligan
D
D
Johnson
C M
CM
Bartle
K D
KD
D004232:Diuretics / Q000032:analysis*; D019075:Electrophoresis, Capillary; D005240:Feasibility Studies; D015203:Reproducibility of Results
D004232:Diuretics