Swimming stress that causes hyperglycemia increases in vivo release of noradrenaline, but not acetylcholine, from the hypothalamus of conscious rats.
The effects of acute swimming stress (10 min) on noradrenaline release from the medial basal hypothalamus (MBH; consisting of the ventromedial and dorsomedial hypothalamus) and acetylcholine release from the lateral hypothalamic area (LHA) were investigated in freely moving rats by using in vivo microdialysis techniques. Serum glucose, noradrenaline and adrenaline concentrations were also determined. Acute swimming stress produced significant hyperglycemia, with increases in serum noradrenaline and adrenaline concentrations. The release of noradrenaline from the MBH was significantly stimulated during the swimming stress. On the other hand, the swimming stress has no significant effect on the release of acetylcholine from the LHA. These findings support the idea that hypothalamic noradrenergic neurons play an important role in the sympathoadrenal hyperglycemic response to stressful stimuli. Moreover, it is suggested that hypothalamic cholinergic neurons are not involved in the responses of serum glucose, noradrenaline and adrenaline concentrations to swimming stress.
Gotoh
M
M
Department of Laboratory Medicine, Aichi Medical University, Nagakute, Japan. mgotoh@amugw.aichi-med-u.ac.jp
Tajima
T
T
Suzuki
Y
Y
Ikari
H
H
Iguchi
A
A
Kakumu
S
S
Hirooka
Y
Y
D000109:Acetylcholine / Q000378:metabolism*; D000704:Analysis of Variance; D000818:Animals; D001786:Blood Glucose / Q000378:metabolism; D004837:Epinephrine / Q000378:metabolism; D006943:Hyperglycemia / Q000503:physiopathology* / Q000523:psychology; D007031:Hypothalamus / Q000378:metabolism*; D008297:Male; D017551:Microdialysis; D009638:Norepinephrine / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar; D013312:Stress, Physiological / Q000503:physiopathology*; D013550:Swimming / Q000502:physiology*
D001786:Blood Glucose; D000109:Acetylcholine; D009638:Norepinephrine; D004837:Epinephrine