Tissue-specific patterns and steady-state concentrations of ecdysteroid receptor and retinoid-X-receptor mRNA during the molt cycle of the fiddler crab, Uca pugilator.
In the fiddler crab, Uca pugilator, we have investigated the temporal expression of receptors in various tissues using probes that encode Uca ecdysteroid receptor (UpEcR) and retinoid-X-receptor (UpRXR) gene homologs. During molt stages C4 through D1-4, UpEcR and UpRXR transcripts are expressed in regenerating limb buds, gills, eyestalks, hypodermis, hepatopancreas, muscle from nonregenerating walking legs, and the large cheliped. Some of these tissues have not previously been recognized as ecdysteroid-target tissues. Levels of ecdysteroids in the hemolymph fluctuate significantly during the molt cycle of U. pugilator. The variation in steady-state concentrations of UpEcR transcripts in tissues from C4 to D1-4 implies molt cycle-related differences in the potential of these tissues to respond to changing titers of ecdysteroids in the hemolymph. In singly autotomized crabs, highest concentrations of UpEcR transcript in some tissues did not coincide with the highest levels of circulating ecdysteroids, suggesting that UpEcR expression in these tissues is not dependent on high ecdysteroid titers and may be induced by low or rising concentrations of ecdysteroids. UpEcR and UpRXR genes were expressed simultaneously in tissues, supporting the possibility of heterodimerization for EcR and RXR in vivo. In some tissues, however, levels of transcripts differed, suggesting other possible receptor interactions. Moreover, UpEcR expression in tissues from multiply autotomized crabs differed from the expression patterns in tissues from singly autotomized crabs.
Chung
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AC
Department of Zoology, University of Oklahoma, Norman 73019, USA.
Durica
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DS
Hopkins
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PM
D000818:Animals; D015152:Blotting, Northern; D003386:Brachyura / Q000235:genetics* / Q000502:physiology; D004441:Ecdysterone / Q000235:genetics*; D007447:Invertebrate Hormones / Q000235:genetics*; D018978:Molting / Q000235:genetics* / Q000502:physiology; D009928:Organ Specificity / Q000235:genetics / Q000502:physiology; D012333:RNA, Messenger / Q000378:metabolism*; D018168:Receptors, Retinoic Acid / Q000235:genetics*; D011987:Receptors, Steroid / Q000235:genetics*; D047488:Retinoid X Receptors; D012260:Ribonucleases; D014157:Transcription Factors / Q000235:genetics*; D014158:Transcription, Genetic
D007447:Invertebrate Hormones; D012333:RNA, Messenger; D018168:Receptors, Retinoic Acid; D011987:Receptors, Steroid; D047488:Retinoid X Receptors; D014157:Transcription Factors; C034808:ecdysteroid receptor; D004441:Ecdysterone; D012260:Ribonucleases