title: Transmembrane-truncated alphavbeta3 integrin retains high affinity for ligand binding: evidence for an 'inside-out' suppressor?
abstract: The molecular mechanisms of alphavbeta3 integrin affinity regulation have important biological implications in tumour development, wound repair and angiogenesis. We expressed, purified and characterized recombinant forms of human alphavbeta3 (r-alphavbeta3) and compared the activation state of these with alphavbeta3 in its cellular environment. The ligand specificity and selectivity of recombinant full-length and double transmembrane truncations of r-alphavbeta3 cloned in BacPAK6 vectors and expressed in Sf9 and High Five insect cells were compared with those of native placental alphavbeta3 and the receptor in situ on the cell surface. r-alphavbeta3 integrins were purified by affinity chromatography from detergent extracts of cells (full-length), and from the culture medium of cells expressing double-truncated r-alphavbeta3. r-alphavbeta3 had the same epitopes, ligand-binding specificities, bivalent cation requirements and susceptibility to RGD-containing peptides as native alphavbeta3. On M21-L4 melanoma cells, alphavbeta3 mediated binding to vitronectin, but not to fibrinogen unless activated with Mn2+. Non-activated alphaIIbbeta3 integrin as control in M21-L-IIb cells had the opposite profile, mediating binding to fibrinogen, but not to vitronectin unless activated with Mn2+. Thus these receptors had moderate to low ligand affinity. In marked contrast, purified alphavbeta3 receptors, with or without transmembrane and cytoplasmic domains, were constitutively of high affinity and able to bind strongly to vitronectin, fibronectin and fibrinogen under physiological conditions. Our data suggest that, in contrast with the positive regulation of alphaIIbbeta3 in situ, intracellular controls lower the affinity of alphavbeta3, and the cytoplasmic domains may act as a target for negative regulators of alphavbeta3 activity.
authors.affiliation: Merck London, MRC Collaborative Centre, 1-3 Burtonhole Lane, Mill Hill, London NW71AD, U.K.
authors.forename: R J
authors.initials: RJ
authors.lastname: Mehta
authors.forename: B
authors.initials: B
authors.lastname: Diefenbach
authors.forename: A
authors.initials: A
authors.lastname: Brown
authors.forename: E
authors.initials: E
authors.lastname: Cullen
authors.forename: A
authors.initials: A
authors.lastname: Jonczyk
authors.forename: D
authors.initials: D
authors.lastname: Güssow
authors.forename: G A
authors.initials: GA
authors.lastname: Luckenbach
authors.forename: S L
authors.initials: SL
authors.lastname: Goodman
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D016367:Baculoviridae / Q000235:genetics; D048429:Cell Size; D002478:Cells, Cultured; D005340:Fibrinogen / Q000378:metabolism; D005353:Fibronectins / Q000378:metabolism; D006801:Humans; D008024:Ligands; D008969:Molecular Sequence Data; D019030:Receptors, Vitronectin / Q000096:biosynthesis / Q000378:metabolism*; D011994:Recombinant Proteins / Q000096:biosynthesis; D018411:Spodoptera; D013499:Surface Properties; D014162:Transfection; D019096:Vitronectin / Q000378:metabolism
chemical_list: D005353:Fibronectins; D008024:Ligands; D019030:Receptors, Vitronectin; D011994:Recombinant Proteins; D019096:Vitronectin; D005340:Fibrinogen