Mature sensory neuron-derived hybrid cell line expressing NGF-dependent neurite extension.
We established a hybrid cell line which extended neurites in the presence of nerve growth factor by fusion between adult mouse sensory neurons and neuroblastoma C1300 cells using emetine and actinomycin D. The serine-phosphorylated 200 and 160 kDa neurofilament proteins were detected in the hybrid cells. In comparison, C1300 cells expressed both subunit of non-phosphorylated neurofilaments at serine residues.
Hikawa
N
N
Department of Physiology, School of Medicine, Yokohama City University, Yokohama, Japan.
Takenaka
T
T
D000818:Animals; D016922:Cellular Senescence / Q000502:physiology; D005260:Female; D006822:Hybrid Cells / Q000502:physiology*; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D009414:Nerve Growth Factors / Q000494:pharmacology*; D016501:Neurites / Q000187:drug effects* / Q000502:physiology*; D016900:Neurofilament Proteins / Q000378:metabolism; D009474:Neurons / Q000502:physiology*; D010750:Phosphoproteins / Q000378:metabolism; D010766:Phosphorylation; D012694:Serine / Q000378:metabolism; D014407:Tumor Cells, Cultured
D009414:Nerve Growth Factors; D016900:Neurofilament Proteins; D010750:Phosphoproteins; D012694:Serine