[The theory and practice of the creation of antisignature oligodeoxyribonucleotides as universal antimicrobial agents (the principles of a drug technology of the 21st century)].
Gene-directed and anti-sense (mRNA-directed) synthetic oligonucleotides (SO) have a common main shortcoming. That is the necessity to introduce intercalators to their composition for the stronger interaction with targets to prevent their separation from the latter by DNA-polymerase and RNA-polymerase complexes which work on genome or with mRNA by ribosomes moving along them. Intercalation leads to considerable loss of SO selectivity in respect to the target. The author substantiates advantages of another type of SO which action is directed to blocking of the function of signature sequences of ribosomal RNA (rRNA) that completely ceases the self-assembly of ribosomal subunits and totally excludes the process of translation and synthesis of proteins. Such type SO advantages are as follows: a) a short chain which includes 8-13 nucleotides altogether; b) absence of the necessity of intercalation; c) high specificity in respect to targets; d) high stability in respect to nucleases action under modification by one of the methods of internucleotide bonds and, e) a possibility to deliver any microorganism to the cells when allowing for auxotrophy of the latter in respect to one or another substance. It is foreseen that antisignature SO can become most promising among the drugs called to block the functions of nucleic acids of the agents of the disease.
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D000900:Anti-Bacterial Agents / Q000138:chemical synthesis* / Q000494:pharmacology; D001483:Base Sequence; D006801:Humans; D008969:Molecular Sequence Data; D016376:Oligonucleotides, Antisense / Q000138:chemical synthesis* / Q000494:pharmacology; D013329:Structure-Activity Relationship; D013678:Technology, Pharmaceutical / Q000639:trends*; D008973:Tenericutes / Q000187:drug effects
D000900:Anti-Bacterial Agents; D016376:Oligonucleotides, Antisense
Teoriia i praktyka stvorennia antysyhnaturnykh olihodezoksyrybonukleotydiv iak universal'nykh antymikrobnykh zasobiv (pryntsypy tekhnolohiĭ likiv XXI viku).