The influence of melatonin on metabolic changes in female rats induced by continuous irradiation and/or administration of 7,12-dimethylbenz/a/anthracene.
Metabolic profile is an important biological marker of neoplastic processes not only in the tumor itself but also in the host organism. The neurohormone melatonin has been implicated in experiments as an oncostatic agent. Female Wistar:Han SPF rats (Velaz, Prague, Czech Republic) were irradiated continuously for 15 days using a daily gamma rays dose of 96 mGy. At the end of exposure one group of rats was administered 5 mg/kg b.w. of dimethylbenz/a/anthracene (DMBA) intragastrically. During the period of exposure to ionizing radiation a part of the animals was supplied with melatonin (M) at a concentration of 20 microliters/ml in drinking water. Selected parameters of lipid and carbohydrate metabolisms and levels of selected hormones were determined 2, 30 and 100 days post-irradiation. The irradiation itself caused only small changes in tissue lipids. The application of a single low dose (subthreshold from the point of view of induction of mammary tumors) of DMBA caused more pronounced changes in nonirradiated animals; of the changes observed an increase in lipids in the liver, triacylglycerols (TG) in the thymus and decrease in myocardial glycogen predominated. The intake (by drinking) of exogenous M prevented the biochemical pattern of fatty liver in animals administered DMBA in both groups, irradiated and nonirradiated. A prolonged effect of exogenous M, demonstrated by prevention of increase in TG in the thymus and of irradiated animals caused by administration of DMBA, was observed. The mechanism of metabolic effect of M is not known. Additional experiments are needed to explain the relationship between the beneficial effect of M on metabolic changes and its presumable oncostatic effect in rats.
Ahlers
I
I
Institute of Animal Physiology, Faculty of Science, P.J. Safárik University, Kosice, Slovakia.
Solár
P
P
Ahlersová
E
E
Kassayová
M
M
Smajda
B
B
D015127:9,10-Dimethyl-1,2-benzanthracene / Q000494:pharmacology*; D000818:Animals; D016588:Anticarcinogenic Agents / Q000494:pharmacology*; D050260:Carbohydrate Metabolism; D005260:Female; D005720:Gamma Rays; D006003:Glycogen / Q000378:metabolism; D050356:Lipid Metabolism; D008550:Melatonin / Q000494:pharmacology*; D009374:Neoplasms, Experimental / Q000517:prevention & control; D009381:Neoplasms, Radiation-Induced / Q000517:prevention & control*; D051381:Rats; D014916:Whole-Body Irradiation
D016588:Anticarcinogenic Agents; D015127:9,10-Dimethyl-1,2-benzanthracene; D006003:Glycogen; D008550:Melatonin