Characterization and determination of human urinary keratan sulfate.
Keratan sulfate was isolated from normal human urine and was characterized by sugar compositional analysis and H-NMR spectroscopy. It was found that KS from human urine is classified as skeletal type (KS-II type chain) with an O-glycosidic linkage between galactosamine and serine (or threonine). H-NMR studies revealed that urinary KS is not a proteoglycan but a polysaccharide (molecular weight is about 5 kDa). The quantitation of human urinary KS by HPLC showed that urinary KS is excreted at constant levels (0.07 +/- 0.015 microgram/mg creatinine).
Toyoda
H
H
Faculty of Pharmaceutical Sciences, Chiba University, Japan.
Demachi
Y
Y
Komoriya
S
S
Furuya
N
N
Toida
T
T
Imanari
T
T
D002241:Carbohydrates / Q000032:analysis; D002851:Chromatography, High Pressure Liquid; D004591:Electrophoresis, Polyacrylamide Gel; D005688:Galactosamine / Q000737:chemistry; D006801:Humans; D007632:Keratan Sulfate / Q000737:chemistry / Q000652:urine*; D009682:Magnetic Resonance Spectroscopy; D008970:Molecular Weight; D011134:Polysaccharides / Q000652:urine*; D012694:Serine / Q000737:chemistry; D013912:Threonine / Q000737:chemistry
D002241:Carbohydrates; D011134:Polysaccharides; D013912:Threonine; D012694:Serine; D005688:Galactosamine; D007632:Keratan Sulfate