[The nature of the interactions of imipramine and serotonin with human lymphocytes].
The work deals with the study of tritium-labeled serotonin (3H-5-hydroxytryptamine, 3H-5-HT) binding with mononuclear leukocytes (lymphocytes) isolated from human peripheral blood. It was shown that 3H-5-HT interacts specifically with the lymphocytes according to the type of reversible, saturated, and temperature-dependent receptor binding. The parameters of binding of the dissociation constant of the ligand-receptor complex and the maximum concentration of the binding centers were determined. It was shown that the tricyclic antidepressant imipramine inhibits the binding of 3H-5-HT with IC50 = 280 nm. The binding of 3H-5-HT with lymphocytes is Na-insensitive but is inhibited by Ca and Mg ions. The interrelation of 3H-5-HT with the lymphocytes differs in nature from the process of reuptake capture of 3H-5-HT by the blood platelets.
Lesnichuk
S A
SA
Department of Biochemistry, Sechenov Moscow Medical Academy, Moscow, Russia.
Katukov
V Iu
VIu
Porodenko
N V
NV
D000328:Adult; D000929:Antidepressive Agents, Tricyclic / Q000097:blood / Q000378:metabolism*; D001667:Binding, Competitive; D002352:Carrier Proteins / Q000378:metabolism; D006801:Humans; D007099:Imipramine / Q000097:blood / Q000378:metabolism*; D066298:In Vitro Techniques; D008024:Ligands; D008214:Lymphocytes / Q000378:metabolism*; D008297:Male; D011955:Receptors, Drug / Q000378:metabolism; D011985:Receptors, Serotonin / Q000378:metabolism; D012701:Serotonin / Q000097:blood / Q000378:metabolism*
D000929:Antidepressive Agents, Tricyclic; D002352:Carrier Proteins; D008024:Ligands; D011955:Receptors, Drug; D011985:Receptors, Serotonin; C027775:imipramine receptor; D012701:Serotonin; D007099:Imipramine
Priroda vzaimodeĭstviia imipramina i serotonina s limfotsitami cheloveka.