Effects of toluene on tonic firing and membrane properties of rat medial vestibular nucleus neurones in vitro.
The effects of toluene on discharge rate and membrane properties of tonically active medial vestibular nucleus (MVN) neurones were investigated in an in vitro slice preparation of the dorsal brainstem of the rat. The majority of the cells (50/56) were inhibited in a dose-dependent manner by toluene. The inhibitory effects of toluene persisted after blockade of synaptic transmission. Complementary patch-clamp recordings showed that toluene caused a hyperpolarisation of 2-5 mV associated with an increase in membrane conductance. These findings indicate that toluene might interfere with specific ion channels or the receptors regulating them along the cell membrane. The effective toluene concentrations used in this experiment are comparable to the concentrations producing vestibulo-ocular disturbances in vivo.
Magnusson
A K
AK
Department of Biomedicine and Surgery, Faculty of Health Sciences, University of Linköping, Sweden. annma@mcb.liu.se
Sulaiman
M R
MR
Dutia
M B
MB
Tham
R
R
D000200:Action Potentials / Q000187:drug effects; D000818:Animals; D066298:In Vitro Techniques; D008297:Male; D008564:Membrane Potentials / Q000187:drug effects; D009474:Neurons / Q000187:drug effects*; D018408:Patch-Clamp Techniques; D051381:Rats; D017207:Rats, Sprague-Dawley; D014050:Toluene / Q000494:pharmacology*; D014726:Vestibular Nuclei / Q000166:cytology / Q000187:drug effects*
D014050:Toluene