title: Identification of an atypical lipoprotein-binding protein from human aortic smooth muscle as T-cadherin.
abstract: We have previously described an atypical lipoprotein-binding protein of about 105 kDa (p105) in membranes of vascular smooth muscle cells (VSMCs) that is distinct from currently known lipoprotein receptors. In the present work we have developed a procedure for purification of p105 from human aortic media. Partial sequencing of purified protein has revealed identity of p105 with human T-cadherin. Anti-peptide antisera raised against human T-cadherin recognized a protein spot corresponding to the purified p105 on two-dimensional Western blots. The antisera also inhibited LDL binding to p105 on ligand blots. We conclude that the 105 kDa lipoprotein-binding protein present in human VSMCs is T-cadherin, an unusual glycosylphosphatidylinositol-anchored member of the cadherin family of cell-cell adhesion proteins.
authors.affiliation: Laboratory of Molecular Endocrinology, Institute of Experimental Cardiology, Cardiology Research Center, Moscow, Russia.
authors.forename: V A
authors.initials: VA
authors.lastname: Tkachuk
authors.forename: V N
authors.initials: VN
authors.lastname: Bochkov
authors.forename: M P
authors.initials: MP
authors.lastname: Philippova
authors.forename: D V
authors.initials: DV
authors.lastname: Stambolsky
authors.forename: E S
authors.initials: ES
authors.lastname: Kuzmenko
authors.forename: M V
authors.initials: MV
authors.lastname: Sidorova
authors.forename: A S
authors.initials: AS
authors.lastname: Molokoedov
authors.forename: V G
authors.initials: VG
authors.lastname: Spirov
authors.forename: T J
authors.initials: TJ
authors.lastname: Resink
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001011:Aorta / Q000737:chemistry*; D015820:Cadherins / Q000737:chemistry / Q000302:isolation & purification* / Q000378:metabolism; D006801:Humans; D008077:Lipoproteins, LDL / Q000378:metabolism*; D051379:Mice; D008807:Mice, Inbred BALB C; D008969:Molecular Sequence Data; D009131:Muscle, Smooth, Vascular / Q000737:chemistry*; D043265:Phosphatidylinositol Diacylglycerol-Lyase; D011485:Protein Binding; D011817:Rabbits; D014357:Trypsin / Q000378:metabolism; D014407:Tumor Cells, Cultured; D010738:Type C Phospholipases / Q000378:metabolism
chemical_list: D015820:Cadherins; C099972:H-cadherin; D008077:Lipoproteins, LDL; D010738:Type C Phospholipases; D014357:Trypsin; D043265:Phosphatidylinositol Diacylglycerol-Lyase