title: Platelet adhesion to native type I collagen fibrils. Role of GPVI in divalent cation-dependent and -independent adhesion and thromboxane A2 generation.
abstract: Three glycoproteins (GPs), namely GPIa-IIa, GPVI, and GPIV, have been recently implicated in platelet-collagen adhesive interactions. We have employed antibodies to these GPs to investigate further their role in platelet adhesion to immobilized monomeric and polymeric fibrillar collagen under static conditions in the presence and the absence of Mg2+. In the presence of Mg2+, each antibody inhibited platelet adhesion to fibrillar collagen from 70 to 85%, especially during the early phase (<15 min), but the inhibitory effects diminished dramatically to 25% or less by 60 min. Combination of anti-GPVI with anti-GPIa-IIa antibodies completely inhibited platelet adhesion at 60 min. Anti-GPIV and anti-GPIa-IIa or anti-GPVI antibodies in combinations were more effective in inhibiting adhesion than was anti-GPIa-IIa or anti-GPVI alone. In the absence of Mg2+, anti-GPVI completely inhibited adhesion at 60 min, while anti-GPIV antibody inhibited adhesion by about 50% and minimal effects were seen with anti-GPIa-IIa, suggesting that GPIa-IIa does not play a significant role in the divalent cation-independent platelet adhesion to immobilized fibrillar collagen. Under either divalent cation-dependent or -independent conditions, platelets adhered to fibrillar collagen were able to secrete contents of both alpha-granules and dense granules and generate thromboxane A2 (TXA2), but platelets adhering to acid soluble monomeric collagen neither secreted their granular contents nor generated TXA2. Although anti-GPVI antibodies were not able to inhibit Mg2+-dependent adhesion, they completely inhibited TXA2 generation under both divalent cation-dependent and -independent conditions. With the other antibodies, TXA2 generation corresponded with the amount of adhesion observed. These results suggest that GPVI is directly associated with the TXA2 generating system during platelet-collagen interaction.
authors.affiliation: Otsuka America Pharmaceutical Inc., Rockville, Maryland 20850, USA.
authors.forename: T
authors.initials: T
authors.lastname: Nakamura
authors.forename: G A
authors.initials: GA
authors.lastname: Jamieson
authors.forename: M
authors.initials: M
authors.lastname: Okuma
authors.forename: J
authors.initials: J
authors.lastname: Kambayashi
authors.forename: N N
authors.initials: NN
authors.lastname: Tandon
mesh_terms: D000818:Animals; D000906:Antibodies / Q000276:immunology; D001792:Blood Platelets / Q000378:metabolism*; D002413:Cations, Divalent; D002448:Cell Adhesion / Q000276:immunology; D003094:Collagen / Q000378:metabolism*; D006801:Humans; D010978:Platelet Factor 4 / Q000378:metabolism; D010980:Platelet Membrane Glycoproteins / Q000276:immunology; D051381:Rats; D012701:Serotonin / Q000378:metabolism; D013928:Thromboxane A2 / Q000096:biosynthesis*; D001620:beta-Thromboglobulin / Q000378:metabolism
chemical_list: D000906:Antibodies; D002413:Cations, Divalent; D010980:Platelet Membrane Glycoproteins; D001620:beta-Thromboglobulin; D012701:Serotonin; D010978:Platelet Factor 4; D013928:Thromboxane A2; D003094:Collagen