title: Peroxide treatment changes activity of non specific esterase in vascular endothelium of isolated pig aorta in vitro.
abstract: The effect of hydrogen peroxide on certain enzymes contained in endothelial cells was investigated in vitro. Specifically in this study the influence of hydrogen peroxide on the non-specific esterase activity in endothelial cells of the perfused pig aorta was examined. We perfused an isolated segment of the pig aorta for 10 h and added 5, 10, 12, 15 ml 10% hydrogen peroxide to 100 ml perfused medium. In unperfused pig aorta 99.34% of the endothelial cells reacted positive. After 10 h of perfusion without hydrogen peroxide administration 93.25% endothelial cells still showed positive enzyme reactivity. In presence of hydrogen peroxide in the perfusion medium the activity of non-specific esterase was progressively inhibited, finally causing complete inactivation.
authors.affiliation: Institute of Pathology, University Leipzig, Germany.
authors.forename: J
authors.initials: J
authors.lastname: Krugmann
authors.forename: M
authors.initials: M
authors.lastname: Neuenfeld
authors.forename: I
authors.initials: I
authors.lastname: Krautschick
mesh_terms: D000251:Adenosine Triphosphatases / Q000378:metabolism; D000818:Animals; D001012:Aorta, Abdominal; D002470:Cell Survival / Q000187:drug effects; D004730:Endothelium, Vascular / Q000166:cytology / Q000187:drug effects / Q000201:enzymology*; D004789:Enzyme Activation / Q000187:drug effects; D004950:Esterases / Q000378:metabolism*; D006861:Hydrogen Peroxide / Q000494:pharmacology*; D066298:In Vitro Techniques; D010477:Perfusion; D013385:Succinate Dehydrogenase / Q000378:metabolism; D013552:Swine
chemical_list: D006861:Hydrogen Peroxide; D013385:Succinate Dehydrogenase; D004950:Esterases; D000251:Adenosine Triphosphatases