title: In vitro phosphorylation of medial vestibular nucleus and prepositus hypoglossi proteins during behavioural recovery from unilateral vestibular deafferentation in the guinea pig.
abstract: Unilateral removal of vestibular nerve input to the vestibular nuclei (e.g. by unilateral labyrinthectomy, UL) results in severe ocular motor and postural disorders which disappear over time (vestibular compensation). We investigated whether recovery of ocular motor function is temporally correlated with changes in protein phosphorylation in the medial vestibular nucleus (MVN) and prepositus hypoglossi (PH; MVN/PH) in vitro. Bilateral MVN/PH were dissected from 48 guinea pigs following decapitation at 10 h, 53 h or 2 weeks post-UL, or -sham operation and frozen. Tissue extracts were incubated with [gamma-32P]ATP +/- Ca2+ plus phorbol 12,13-dibutyrate and phosphatidylserine. UL resulted in a significant bilateral increase in the 32P-incorporation into a 65-85 kDa band (probably the myristoylated alanine-rich C kinase substrate, MARCKS) in compensated animals (53 h post-UL) under conditions which favoured the activation of protein kinase C. Under identical conditions, the labelling of a 42-49 kDa protein (P46) was increased significantly in the bilateral MVN/PH between either 10 h or 53 h and 2 weeks post-UL; there were no significant changes over time in sham controls. These results show that later stages of vestibular compensation are accompanied by changes in the phosphorylation of several likely protein kinase C substrates in the MVN/PH in vitro.
authors.affiliation: Department of Psychology, Otago Medical School, University of Otago, Dunedin, New Zealand.
authors.forename: A J
authors.initials: AJ
authors.lastname: Sansom
authors.forename: V A
authors.initials: VA
authors.lastname: Brent
authors.forename: P E
authors.initials: PE
authors.lastname: Jarvie
authors.forename: C L
authors.initials: CL
authors.lastname: Darlington
authors.forename: P F
authors.initials: PF
authors.lastname: Smith
authors.forename: R
authors.initials: R
authors.lastname: Laverty
authors.forename: J A
authors.initials: JA
authors.lastname: Rostas
mesh_terms: D000344:Afferent Pathways / Q000502:physiology; D000818:Animals; D001522:Behavior, Animal / Q000502:physiology*; D003714:Denervation; D004789:Enzyme Activation; D005260:Female; D007839:Functional Laterality / Q000502:physiology*; D006168:Guinea Pigs; D007002:Hypoglossal Nerve / Q000502:physiology*; D008297:Male; D010766:Phosphorylation; D011184:Postoperative Period; D011494:Protein Kinases / Q000187:drug effects*; D013268:Stimulation, Chemical; D014726:Vestibular Nuclei / Q000378:metabolism*
chemical_list: D011494:Protein Kinases