title: A detailed protocol for the measurement of TGF-beta1 in human blood samples.
abstract: Quantification of the multifunctional cytokine Transforming Growth Factor-beta1 (TGF-beta1) in blood samples has aroused increasing interest in recent years, since an abnormal regulation of this cytokine appears to play a key role in the pathogenesis of different diseases, such as autoimmundiseases or malignant tumors. The measurement of TGF-beta1 is complicated by a lot of problems concerning the collection, preparation and handling of blood samples, the platelet contamination, and the TGF-beta1 activation procedure. Here, we recommend detailed instructions for measurement of TGF-beta1 in blood plasma samples which should be followed to exclude the determination of false positive or negative results.
authors.forename: D
authors.initials: D
authors.lastname: Reinhold
authors.forename: U
authors.initials: U
authors.lastname: Bank
authors.forename: F
authors.initials: F
authors.lastname: Bühling
authors.forename: U
authors.initials: U
authors.lastname: Junker
authors.forename: J
authors.initials: J
authors.lastname: Kekow
authors.forename: E
authors.initials: E
authors.lastname: Schleicher
authors.forename: S
authors.initials: S
authors.lastname: Ansorge
mesh_terms: D006801:Humans; D016212:Transforming Growth Factor beta / Q000097:blood*
chemical_list: D016212:Transforming Growth Factor beta