title: A chemiluminescent FIA biosensor for phosphate ion monitoring using pyruvate oxidase.
abstract: We have constructed an automatic phosphate ion sensing system for the quality control of drinking water. The analyte was detected using the phosphate ion-dependent pyruvate oxidase reaction and the hydrogen peroxide produced was detected by luminol chemiluminescence catalyzed by Arthromyces ramosus peroxidase. We obtained a detection limit of 0.16 microM phosphate ion (5 ppb phosphorus) and it was possible to detect 0.32 microM phosphate ion for 48 days using pyruvate oxidase immobilized on Chitopearl BCW-2601 beads. An excellent correlation (r2 = 1.00) was obtained between the results obtained using our phosphate ion sensor and those using a modified Molybdenum Blue method.
authors.affiliation: Research Center for Advanced Science and Technology, University of Tokyo, Japan.
authors.forename: H
authors.initials: H
authors.lastname: Nakamura
authors.forename: K
authors.initials: K
authors.lastname: Ikebukuro
authors.forename: S
authors.initials: S
authors.lastname: McNiven
authors.forename: I
authors.initials: I
authors.lastname: Karube
authors.forename: H
authors.initials: H
authors.lastname: Yamamoto
authors.forename: K
authors.initials: K
authors.lastname: Hayashi
authors.forename: M
authors.initials: M
authors.lastname: Suzuki
authors.forename: I
authors.initials: I
authors.lastname: Kubo
mesh_terms: D001331:Automation; D015374:Biosensing Techniques; D004356:Drug Storage; D017022:Flow Injection Analysis; D005618:Fresh Water / Q000737:chemistry*; D007202:Indicators and Reagents; D008163:Luminescent Measurements; D010710:Phosphates / Q000032:analysis*; D011771:Pyruvate Oxidase; D011786:Quality Control
chemical_list: D007202:Indicators and Reagents; D010710:Phosphates; D011771:Pyruvate Oxidase