title: 11Beta-hydroxysteroid dehydrogenase activity in progesterone biotransformation by the filamentous fungus Cochliobolus lunatus.
abstract: Progesterone biotransformation was examined in relation to hydroxylating and dehydrogenating enzymes of Cochliobolus lunatus. 11beta-hydroxysteroid dehydrogenase activity (11beta-HSD) was located in cytosolic fraction and was NADP-dependent, inducible by progesterone and apparently uni-directional. Several inhibitors of 11beta-hydroxysteroid dehydrogenase were tested; furosemide, glycyrrhizic-acid and carbenoxolone did not influence the dehydrogenation of 11beta-hydroxy-4-pregnene-3,20-dione to 4-pregnene-3,11,20-trione, although grapefruit juice significantly reduced the rate of progesterone hydroxylation.
authors.affiliation: Medical Centre for Molecular Biology, Institute of Biochemistry, Medical Faculty, Ljubljana, Slovenia.
authors.forename: M
authors.initials: M
authors.lastname: Vitas
authors.forename: T
authors.initials: T
authors.lastname: Pajic
authors.forename: S L
authors.initials: SL
authors.lastname: Kelly
authors.forename: R
authors.initials: R
authors.lastname: Komel
mesh_terms: D042842:11-beta-Hydroxysteroid Dehydrogenases; D001203:Ascomycota / Q000201:enzymology*; D001628:Beverages; D001711:Biotransformation; D002957:Citrus; D003577:Cytochrome P-450 Enzyme System / Q000378:metabolism; D006913:Hydroxysteroid Dehydrogenases / Q000378:metabolism*; D011374:Progesterone / Q000493:pharmacokinetics*
chemical_list: D011374:Progesterone; D003577:Cytochrome P-450 Enzyme System; D006913:Hydroxysteroid Dehydrogenases; D042842:11-beta-Hydroxysteroid Dehydrogenases