Arachidonic-acid-selective cytosolic phospholipase A2 is involved in gastrin-induced AR4-2J-cell proliferation.
Gastrin/CCK(B) G protein-coupled receptors have been shown to mediate proliferation stimulated by their endogenous ligands. The present study demonstrates the proliferative effect of arachidonic acid on AR4-2J cells. Gastrin induces an [3H]arachidonic-acid release in a dose-dependent manner. The use of a specific inhibitor of cPLA2, AACOCF3 established the involvement of a cPLA2 in the proliferative effect of gastrin. The results also demonstrate that a cytosolic high-molecular-weight PLA2 is activated by gastrin in AR4-2J cells.
Ghrib
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INSERM U.151, Groupe de Recherche de Biologie et Pathologie Digestive, Institut Louis Bugnard, CHU Rangueil, Toulouse, France. pradayrol@rangueil.inserm.fr
Pyronnet
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Bastié
M J
MJ
Fagot-Revurat
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P
Pradayrol
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L
Vaysse
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N
D000818:Animals; D016718:Arachidonic Acid / Q000502:physiology*; D002118:Calcium / Q000378:metabolism; D002455:Cell Division; D002460:Cell Line; D003600:Cytosol / Q000201:enzymology; D004789:Enzyme Activation; D005755:Gastrins / Q000494:pharmacology*; D010741:Phospholipases A / Q000502:physiology*; D054467:Phospholipases A2; D051381:Rats
D005755:Gastrins; D016718:Arachidonic Acid; D010741:Phospholipases A; D054467:Phospholipases A2; D002118:Calcium