title: Experience with a broth culture technique for diagnosis of bacterial keratitis.
abstract: OBJECTIVE
  To determine if a broth culture technique is a practical means for bacteriological investigation of keratitis.
  
  
  METHODS
  Twenty-seven eyes of 27 patients with a clinical diagnosis of bacterial keratitis were included in a prospective and non-comparative study at a Danish referral hospital. A corneal scrape was inoculated directly into broth medium which was transferred to the diagnostic laboratory for incubation and subculture.
  
  
  RESULTS
  Culture was negative in 4 patients, and 19 of the remaining 23 patients had a pure growth of either Pseudomonas aeruginosa (n = 8), Staphylococcus aureus (n = 2), Streptococcus pneumoniae (n = 2), Haemophilus influenzae biotype III (n = 1), Moraxella species (n = 1), Corynebacterium species (n = 1), or coagulase-negative staphylococci (n = 4). In 4 patients there was a mixed gram-positive growth. There was no association between microbiological findings and previous topical antibiotic therapy. Contamination and lack of quantitative assessment of growth proved not to be a problem.
  
  
  CONCLUSIONS
  By broth culture technique we identified a definite pathogen (P. aeruginosa, S. aureus or S. pneumoniae) in 44% of patients (95% binomial confidence limits: 25-65%). The technique may replace the standard technique of direct plate culture under circumstances where it is difficult to keep a supply of fresh media or transport inoculated plates.
authors.affiliation: Department of Clinical Microbiology, Aalborg Hospital, Denmark.
authors.forename: H C
authors.initials: HC
authors.lastname: Schønheyder
authors.forename: J K
authors.initials: JK
authors.lastname: Pedersen
authors.forename: K
authors.initials: K
authors.lastname: Naeser
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D001419:Bacteria / Q000302:isolation & purification; D001431:Bacteriological Techniques; D003315:Cornea / Q000382:microbiology*; D003470:Culture Media; D015818:Eye Infections, Bacterial / Q000175:diagnosis / Q000382:microbiology*; D005260:Female; D006801:Humans; D007634:Keratitis / Q000175:diagnosis / Q000382:microbiology*; D008297:Male; D008875:Middle Aged; D011446:Prospective Studies
chemical_list: D003470:Culture Media