AIM-1: a mammalian midbody-associated protein required for cytokinesis.
Mitosis is a highly coordinated process that assures the fidelity of chromosome segregation. Errors in this process result in aneuploidy which can lead to cell death or oncogenesis. In this paper we describe a putative mammalian protein kinase, AIM-1 (Aurora and Ipl1-like midbody-associated protein), related to Drosophila Aurora and Saccharomyces cerevisiae Ipl1, both of which are required for chromosome segregation. AIM-1 message and protein accumulate at G2/M phase. The protein localizes at the equator of central spindles during late anaphase and at the midbody during telophase and cytokinesis. Overexpression of kinase-inactive AIM-1 disrupts cleavage furrow formation without affecting nuclear division. Furthermore, cytokinesis frequently fails, resulting in cell polyploidy and subsequent cell death. These results strongly suggest that AIM-1 is required for proper progression of cytokinesis in mammalian cells.
Terada
Y
Y
Louis Pasteur Center for Medical Research, Department of Molecular Biology, 103-5 Tanaka Monzencho, Sakyo-ku, Kyoto 606. terada@fas.harvard.edu
Tatsuka
M
M
Suzuki
F
F
Yasuda
Y
Y
Fujita
S
S
Otsu
M
M
D000595:Amino Acid Sequence; D000818:Animals; D064127:Aurora Kinases; D002453:Cell Cycle / Q000235:genetics / Q000502:physiology; D018797:Cell Cycle Proteins / Q000235:genetics / Q000502:physiology; D002455:Cell Division / Q000235:genetics / Q000502:physiology; D018076:DNA, Complementary / Q000032:analysis / Q000235:genetics; D016195:G2 Phase / Q000235:genetics / Q000502:physiology; D015870:Gene Expression / Q000235:genetics; D008938:Mitosis / Q000235:genetics / Q000502:physiology; D008969:Molecular Sequence Data; D011494:Protein Kinases / Q000235:genetics* / Q000302:isolation & purification / Q000502:physiology; D017346:Protein Serine-Threonine Kinases; D012333:RNA, Messenger / Q000032:analysis / Q000235:genetics; D051381:Rats; D017386:Sequence Homology, Amino Acid; D008941:Spindle Apparatus / Q000737:chemistry; D014018:Tissue Distribution
D018797:Cell Cycle Proteins; D018076:DNA, Complementary; D012333:RNA, Messenger; D011494:Protein Kinases; D064127:Aurora Kinases; D017346:Protein Serine-Threonine Kinases