title: Immunoglobulin E-induced passive sensitization of human airways: an immunohistochemical study.
abstract: In vivo, IgE production is related to bronchial hyperresponsiveness and, in vitro, passive sensitization of human airways with asthmatic serum containing a high concentration of IgE enhances the contractile response to a variety of agonists. However, cell types implicated in this IgE sensitization are not fully determined. The aim of this study was to determine IgE-bearing cells during passive sensitization with special reference to mast cells. Peripheral bronchi were dissected out from 10 lung specimens obtained at thoracotomy and processed into glycolmethacrylate resin. Sections, each 2 microm thick, were passively sensitized by incubation for 2 h at 37 degrees C in either buffer supplemented with monoclonal IgE or asthmatic serum with a high concentration of IgE (> or = 1,000 IU/ml). Immunohistochemistry was performed using monoclonal antibodies directed against the epsilon chain, and markers of the various IgE-bearing cells (e.g., AA1, antichymase). The number of IgE-bearing cells was significantly higher in passively sensitized specimens as compared with nonsensitized specimens (6.63 +/- 1.71 versus 4.29 +/- 1.35/mm2; p = 0.013, n = 10). Mast cells represented 65% of IgE-bearing cells, 41.6 and 23.4% for TC and T subtypes, respectively. These results indicate that mast cell is the main cell type involved in IgE-induced passive sensitization. The involvement of mast cell-derived tryptase in the mechanisms of IgE-related hyperresponsiveness should be further examined.
authors.affiliation: Laboratoire de Physiologie Cellulaire Respiratoire, Université Victor Ségalen Bordeaux 2, France.
authors.forename: P
authors.initials: P
authors.lastname: Berger
authors.forename: A F
authors.initials: AF
authors.lastname: Walls
authors.forename: R
authors.initials: R
authors.lastname: Marthan
authors.forename: J M
authors.initials: JM
authors.lastname: Tunon-de-Lara
mesh_terms: D000328:Adult; D000368:Aged; D001249:Asthma / Q000097:blood; D001980:Bronchi / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D005260:Female; D006801:Humans; D007116:Immunization, Passive; D007073:Immunoglobulin E / Q000378:metabolism / Q000502:physiology*; D007150:Immunohistochemistry; D008297:Male; D008407:Mast Cells / Q000378:metabolism; D008875:Middle Aged
chemical_list: D007073:Immunoglobulin E