title: [Prognostic significance of DNA content measured by flow cytometry in supraglottic laryngeal carcinoma].
abstract: Eighty-five patients with squamous that were cell carcinoma of the supraglottis treated surgically in the Department of Otolaryngology at the Marqués de Valdecilla Hospital in Santander (Spain) over a 10-year period from January 1, 1978 to December 31, 1987 were studied retrospectively. In 81 cases a flow cytometric study was made from the surgical specimen included in paraffin blocks, to determine the DNA index and the percentage of cells in the S phase of the cellular cycle. An statistical analysis was made to determine the correlation between these parameters and survival was analyzed using the Kaplan-Merer method. Survival curves were compared using the log-rank test. In 67 cases (82.7%) DNA content was diploid and in 14 cases (17.3%) it was aneuploid. The percentage of cells in S phase was 3.8 and 55.4, with a mean value of 15.19. Comparison of with the five these parameters years survival showed no statistical differences between groups, although tumors with more than 19% S phase had worse prognosis (p < 0.1) and tumors in advanced stages were more frequently aneuploid and had higher S phase values (p = 0.1).
authors.affiliation: Servicio de ORL, Hospital Sierrallana, Torrelavega.
authors.forename: C
authors.initials: C
authors.lastname: Morales Angulo
authors.forename: F
authors.initials: F
authors.lastname: Fernández Fernández
authors.forename: F
authors.initials: F
authors.lastname: Val Bernal
authors.forename: L
authors.initials: L
authors.lastname: Buelta
authors.forename: L
authors.initials: L
authors.lastname: García-Castrillo
authors.forename: J
authors.initials: J
authors.lastname: Rama Quintela
mesh_terms: D000328:Adult; D000368:Aged; D002294:Carcinoma, Squamous Cell / Q000235:genetics* / Q000401:mortality / Q000601:surgery; D004273:DNA, Neoplasm / Q000032:analysis*; D005434:Flow Cytometry / Q000379:methods*; D006801:Humans; D007822:Laryngeal Neoplasms / Q000235:genetics* / Q000401:mortality / Q000601:surgery; D008297:Male; D008875:Middle Aged; D011003:Ploidies; D011237:Predictive Value of Tests; D012189:Retrospective Studies; D015996:Survival Rate
chemical_list: D004273:DNA, Neoplasm
vernacular_title: Valor pronóstico del contenido de ADN por citometría de flujo en el carcinoma supraglótico de laringe.