Fine chemical structure analysis of oligosaccharides produced by an ulvan-lyase degradation of the water-soluble cell-wall polysaccharides from Ulva sp, (Ulvales, Chlorophyta).
A marine bacterium degrading the water-soluble cell wall polysaccharides from Ulva sp. (ulvan) has been isolated. The good correlation between ulvan degradation monitored by reducing-power, UV absorbance and viscosimetry, indicated that the crude enzymatic extract contains essentially an endo-ulvan lyase activity. This activity was rapidly inhibited by the reaction products which consisted of a series of ulvanobiouronic acid A 3-sulfate [-->4)-beta-D-GlcpA-(1-->4)-alpha-L-Rhap 3-sulfate-(1-->]n with 4-deoxy-L-threo-hex-4-enopyranosiduronic acid at the non-reducing end. Other deviant repeating structures with beta-D-Xylp or alpha-IdopA replacing beta-D-GlcpA in the repeating ulvanobiouronic acid disaccharide and the presence of two consecutive (1-->4) linked beta-D-Glc pA demonstrated the great variability and complexity of ulvan chemical structure.
Lahaye
M
M
Institut National de la Recherche Agronomique, Laboratoire de Biochimie et Technologie des Glucides, Nantes, France.
Brunel
M
M
Bonnin
E
E
D002236:Carbohydrate Conformation; D002240:Carbohydrate Sequence; D002473:Cell Wall / Q000737:chemistry; D000460:Chlorophyta / Q000737:chemistry*; D007700:Kinetics; D008969:Molecular Sequence Data; D009844:Oligosaccharides / Q000737:chemistry*; D011133:Polysaccharide-Lyases / Q000378:metabolism*; D011134:Polysaccharides / Q000737:chemistry* / Q000302:isolation & purification; D012995:Solubility; D014783:Viscosity
D009844:Oligosaccharides; D011134:Polysaccharides; D011133:Polysaccharide-Lyases; C110941:ulvan-lyase