Polypeptide pattern of mRNA isolated from polysomal and non-polysomal mRNA-protein(mRNP) fractions in rat myoblast.
Polypeptides synthesized from polysome-bound and unbound fractions of polyA mRNA were studied by in-vivo cells labelling and cDNA-mRNA hybrid-arrest translation. The polypeptides synthesized from unbound(free) fraction appeared to be significantly reduced in the absence of cycloheximide in L6 myoblast. The cDNA excess-mRNA hybrid-arrest translation exhibited rare polypeptide sequences in the unbound fractions.
Bhattacharya
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Department of Medicine, Memorial University of Newfoundland, St. John's, Canada.
D000818:Animals; D005591:Chemical Fractionation; D009132:Muscles / Q000378:metabolism*; D010455:Peptides / Q000032:analysis*; D011132:Polyribosomes / Q000737:chemistry*; D012333:RNA, Messenger / Q000737:chemistry* / Q000302:isolation & purification; D051381:Rats; D012261:Ribonucleoproteins / Q000378:metabolism*
D010455:Peptides; D012333:RNA, Messenger; D012261:Ribonucleoproteins