title: Optimal 96-well plate set-up to avoid ethanol volatility when assessing ethanol cytotoxicity.
abstract: An optimal design has been established that maintains a constant level of ethanol when utilizing specific wells within a 96-well culture plate. A control (non-ethanol-containing) column is also included within each plate for direct comparisons of the cytotoxic effects of a given concentration of ethanol.
authors.affiliation: Department of Neuroscience, University of Florida College of Medicine, Gainesville 32610-0244, USA. mitchell@ufbi.ufl.edu
authors.forename: J J
authors.initials: JJ
authors.lastname: Mitchell
authors.forename: M
authors.initials: M
authors.lastname: Paiva
authors.forename: M B
authors.initials: MB
authors.lastname: Heaton
mesh_terms: D000818:Animals; D002470:Cell Survival / Q000187:drug effects; D046508:Culture Techniques; D000431:Ethanol / Q000494:pharmacology / Q000633:toxicity*; D006624:Hippocampus / Q000187:drug effects / Q000201:enzymology; D051381:Rats; D018675:Toxicity Tests / Q000379:methods*; D014835:Volatilization
chemical_list: D000431:Ethanol