title: [Relation between common allelic variation in a cross-sectional longitudinal population study. Vitamin D receptor locus, bone mineral density and postmenopausal bone loss].
abstract: The aim of this study was to determine whether common allelic variation at the vitamin D receptor locus is related to bone mineral density and postmenopausal bone loss. Five hundred and ninety-nine healthy women aged 27 to 72 and 125 women with low bone mass aged 55-77 were measured once in a cross-sectional design. Furthermore, 136 women aged 45-54 were followed longitudinally for 18 years. The vitamin D receptor genotypes were determined using Polymerase Chain Reaction-Restriction Fragment Length Polymorphism analysis for three different polymorphic restriction sites (BsmI, ApaI and TaqI). Bone mineral density was measured by photon and X-ray absorptiometry. The cross-sectional study showed no significant relationship between vitamin D receptor genotype and bone mineral density. The maximum difference between homozygotes was 1.3%, P = 0.33, N = 723. The low bone mineral density group had virtually the same genotype frequencies as the normal bone mineral density group. The results of the longitudinal study showed that vitamin D receptor genotype was neither related to early postmenopausal bone loss: age 51-53 (total bone loss at the lower forearm: -3.6% [3.6%], mean [standard deviation]), late postmenopausal bone loss: age 63-69 (total bone loss at the hip: -6.2% [8.7%]) nor to long term postmenopausal bone loss: age 51-69 (total bone loss at the lower forearm: -24.5% [11.4%]). Using analysis of variance to test for differences in the rates of bone loss between the vitamin D receptor genotypes, P values ranged from 0.07 to 0.79. We conclude that common allelic variation at the vitamin D receptor locus as defined by the endonucleases ApaI, BsmI and TaqI is neither related to bone mineral density nor to the rate of bone loss in healthy postmenopausal Danish women.
authors.affiliation: Den Danske Forskningsfond, Ballerup.
authors.forename: H L
authors.initials: HL
authors.lastname: Jørgensen
authors.forename: J
authors.initials: J
authors.lastname: Schøller
authors.forename: J C
authors.initials: JC
authors.lastname: Sand
authors.forename: M
authors.initials: M
authors.lastname: Bjuring
authors.forename: C
authors.initials: C
authors.lastname: Hassager
authors.forename: C
authors.initials: C
authors.lastname: Christiansen
mesh_terms: D000328:Adult; D000368:Aged; D000483:Alleles; D015519:Bone Density; D003430:Cross-Sectional Studies; D003718:Denmark; D005260:Female; D014644:Genetic Variation; D005838:Genotype; D006801:Humans; D008137:Longitudinal Studies; D008875:Middle Aged; D015663:Osteoporosis, Postmenopausal / Q000235:genetics*; D018167:Receptors, Calcitriol / Q000235:genetics*
chemical_list: D018167:Receptors, Calcitriol
vernacular_title: Relation mellem simpel allelisk variation i et tvaersnits- og longitudinelt befolkningsstudie. Vitamin D-receptorlocus, knoglemineraldensitet og postmenopausalt knogletab.