Localization of SH-PTP1 to synaptic vesicles: a possible role in neurotransmission.
The tyrosine kinase pp60src is known to phosphorylate synaptophysin and in doing so may regulate neurotransmitter release. The tyrosyl phosphorylated state of synaptophysin is dependent on pp60src kinase and the unknown protein tyrosine phosphate phosphohydrolase (PTPase, EC 3.1.3.48). Here we report the protein tyrosine phosphate phosphohydrolase SH-PTP1, to associate with synaptic vesicles and interact with synaptophysin. These studies identify SH-PTP1 as a new member of the secretory machinery at the nerve terminal and suggest its involvement in neurotransmission.
Jena
B P
BP
Department of Surgery, Yale University School of Medicine, New Haven, CT 06510, USA.
Webster
P
P
Geibel
J P
JP
Van den Pol
A N
AN
Sritharan
K C
KC
D000818:Animals; D015153:Blotting, Western; D007031:Hypothalamus / Q000201:enzymology; D047908:Intracellular Signaling Peptides and Proteins; D016253:Microscopy, Immunoelectron; D009419:Nerve Tissue Proteins / Q000032:analysis* / Q000502:physiology; D009474:Neurons / Q000201:enzymology; D010766:Phosphorylation; D011499:Protein Processing, Post-Translational; D054592:Protein Tyrosine Phosphatase, Non-Receptor Type 11; D053824:Protein Tyrosine Phosphatase, Non-Receptor Type 6; D017027:Protein Tyrosine Phosphatases / Q000032:analysis* / Q000502:physiology; D016392:Proto-Oncogene Proteins pp60(c-src) / Q000502:physiology; D051381:Rats; D017207:Rats, Sprague-Dawley; D009435:Synaptic Transmission; D013572:Synaptic Vesicles / Q000201:enzymology*; D016708:Synaptophysin / Q000378:metabolism
D047908:Intracellular Signaling Peptides and Proteins; D009419:Nerve Tissue Proteins; D016708:Synaptophysin; D016392:Proto-Oncogene Proteins pp60(c-src); D054592:Protein Tyrosine Phosphatase, Non-Receptor Type 11; D053824:Protein Tyrosine Phosphatase, Non-Receptor Type 6; D017027:Protein Tyrosine Phosphatases; C516727:Ptpn11 protein, rat; C509250:Ptpn6 protein, rat