title: Determination of HbA1c in the presence of haemoglobin variants: comparison of three HPLC techniques.
abstract: The presence of Hb variants may cause analytical interference in HbA1c values measured with the HPLC techniques currently used in Italian laboratories. In this study performance of a new HPLC system, (HA-8140, Menarini) was compared with two other traditional HPLC systems (HA-8121, Menarini and Diamat, Bio-Rad). The HA-8140 system detects the Hb variant possibly present in an independent peak. The integration area of such a peak is not computed in the calculation of the percentage value of HbA1c. In this manner the underestimation of values obtained with traditional HPLC systems is avoided.
authors.affiliation: Laboratorio di Chimica Clinica ed Ematologia, Ospedale S. Bortolo, Vicenza, Italy.
authors.forename: M
authors.initials: M
authors.lastname: Carta
authors.forename: G
authors.initials: G
authors.lastname: Dall'Olio
authors.forename: G
authors.initials: G
authors.lastname: Soffiati
mesh_terms: D001786:Blood Glucose / Q000378:metabolism; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D003920:Diabetes Mellitus / Q000503:physiopathology; D006442:Glycated Hemoglobin / Q000032:analysis*; D006453:Hemoglobinopathies / Q000503:physiopathology*; D006454:Hemoglobins / Q000032:analysis; D006579:Heterozygote; D006720:Homozygote; D006801:Humans; D007558:Italy
chemical_list: D001786:Blood Glucose; D006442:Glycated Hemoglobin; D006454:Hemoglobins