[Demonstration of the angiogenic cytokine vascular endothelial growth factor (VEGF) by non radioactive in situ hybridization].
In tumor angiogenesis Vascular Endothelial Growth Factor (VEGF) has an important role due to its target cell specificity. It is expressed by the tumor and effects on a paracrine pathway. To increase the understanding of its regulation, it is necessary to identify those cells releasing VEGF. This can be done by in situ hybridization (ISH). In this paper we present a protocol for non-radioactive ISH for VEGF colonic tissue. With this protocol it is possible to perform hybridization within one day.
Strik
M W
MW
Klinik für Chirurgie der Medizinischen Universität zu Lübeck, Deutschland.
Laus
C
C
Duchrow
M
M
Broll
R
R
Bruch
H P
HP
D003106:Colon / Q000378:metabolism / Q000473:pathology*; D015179:Colorectal Neoplasms / Q000378:metabolism / Q000473:pathology*; D018076:DNA, Complementary; D016228:Endothelial Growth Factors / Q000032:analysis / Q000096:biosynthesis*; D006801:Humans; D017403:In Situ Hybridization / Q000379:methods; D008222:Lymphokines / Q000032:analysis / Q000096:biosynthesis*; D009389:Neovascularization, Pathologic; D042461:Vascular Endothelial Growth Factor A; D042442:Vascular Endothelial Growth Factors
D018076:DNA, Complementary; D016228:Endothelial Growth Factors; D008222:Lymphokines; D042461:Vascular Endothelial Growth Factor A; D042442:Vascular Endothelial Growth Factors
Nachweis des angiogenen Zytokins Vascular Endothelial Growth Factor (VEGF) durch nicht radioaktive In-situ-Hybridisierung.