title: [Optimization of the polymerase chain reaction (PCR) for detection and characterization of shigatoxin producing Escherichia coli (STEC) in food].
abstract: The polymerase chain reaction (PCR) was improved to detect shigatoxin producing Escherichia coli (STEC) in milk. Numbers of colony forming units (cfu) in test samples, concentrations and types of primers, amount of MgCl2, types of thermostable DNA-Polymerase, and cycling programs were modified up to obtain the cleanest electrophoretic pattern and the highest sensitivity. Experimental conditions for further characterization of STEC-isolates by means of PCR are given by summarizing data from literature.
authors.affiliation: Bundesinstitut für gesundheitlichen Verbraucherschutz und Veterinärmedizin, Standorte in Dessau.
authors.forename: P
authors.initials: P
authors.lastname: Gallien
authors.forename: H
authors.initials: H
authors.lastname: Richter
authors.forename: C
authors.initials: C
authors.lastname: Much
authors.forename: I
authors.initials: I
authors.lastname: Fröbe
authors.forename: K W
authors.initials: KW
authors.lastname: Perlberg
authors.forename: D
authors.initials: D
authors.lastname: Protz
mesh_terms: D000818:Animals; D001427:Bacterial Toxins / Q000096:biosynthesis*; D002417:Cattle; D004926:Escherichia coli / Q000302:isolation & purification*; D005516:Food Microbiology; D008892:Milk / Q000382:microbiology*; D016133:Polymerase Chain Reaction / Q000379:methods*; D022603:Shiga Toxins
chemical_list: D001427:Bacterial Toxins; D022603:Shiga Toxins
vernacular_title: Optimierung der Polymerasekettenreaktion (PCR) zum Nachweis und zur Charakterisierung von Shigatoxin produzierenden Escherichia coli (STEC) in Lebensmitteln.