title: Radiosensitive melanoma cell line with mutation of the gene for ataxia telangiectasia.
abstract: The human melanoma cell lines MM96L, A2058 and HT144 were examined for sensitivity to ionizing radiation and UVB radiation. HT144 demonstrated a significant increase in sensitivity to ionizing and UVB radiation compared with the MM96L and A2058 cells. Sensitivity to both agents was associated with susceptibility to apoptosis. Using a protein truncation assay, a mutation for the gene for ataxia telangiectasia (ATM) was identified in HT144 cells. This was confirmed to be a homozygous mutation by subsequent sequencing of the abnormal region. Protein truncation assay of the other two cell lines showed no abnormality. The results suggest that somatic mutation of the A-T gene may be important in determining tumour radiosensitivity.
authors.affiliation: Queensland Radium Institute - Mater Centre, South Brisbane, Australia.
authors.forename: J
authors.initials: J
authors.lastname: Ramsay
authors.forename: G
authors.initials: G
authors.lastname: Birrell
authors.forename: K
authors.initials: K
authors.lastname: Baumann
authors.forename: A
authors.initials: A
authors.lastname: Bodero
authors.forename: P
authors.initials: P
authors.lastname: Parsons
authors.forename: M
authors.initials: M
authors.lastname: Lavin
mesh_terms: D017209:Apoptosis; D001260:Ataxia Telangiectasia / Q000235:genetics*; D064007:Ataxia Telangiectasia Mutated Proteins; D018797:Cell Cycle Proteins; D002470:Cell Survival / Q000528:radiation effects; D004268:DNA-Binding Proteins; D006801:Humans; D008545:Melanoma / Q000235:genetics*; D009154:Mutation; D017346:Protein Serine-Threonine Kinases; D011506:Proteins / Q000032:analysis / Q000235:genetics*; D014407:Tumor Cells, Cultured / Q000528:radiation effects; D014410:Tumor Stem Cell Assay; D025521:Tumor Suppressor Proteins; D014466:Ultraviolet Rays
chemical_list: D018797:Cell Cycle Proteins; D004268:DNA-Binding Proteins; D011506:Proteins; D025521:Tumor Suppressor Proteins; C576324:ATM protein, human; D064007:Ataxia Telangiectasia Mutated Proteins; D017346:Protein Serine-Threonine Kinases