title: Prolonged bleeding time with defective platelet filopodia formation in the Wistar Furth rat.
abstract: Hereditary macrothrombocytopenia is a hallmark of Wistar Furth (WF) rats. In addition, a platelet/megakaryocyte alpha granule defect, similar to that of patients with gray platelet syndrome, is present. Several observations indicate cytoskeletal abnormalities in WF platelets and megakaryocytes, suggesting the potential for functional defects in hemostatic processes requiring cytoskeletal reorganization, such as platelet adhesion and spreading. However, no bleeding abnormality has been noted. Here, we report a prolonged bleeding time (>30 minutes in 10 of 11 rats tested) with defective clot formation in the WF strain. Prolonged bleeding time can result from defects in platelet adhesion, aggregation, or the release reaction. Because aggregation to collagen and adenosine diphosphate were reported to be normal, we determined whether WF rat platelets are defective in their ability to adhere to substrates. Platelet adherence and spreading was evaluated from 30 seconds to 30 minutes on Formvar-coated, carbon-stabilized grids or poly-L-lysine-coated glass coverslips by transmission electron microscopy or immunofluorescence, respectively, and scanning electron microscopy. We classified the adhered platelets according to their pattern of spreading, ie, rounded, rounded or spreading with short filopodia, spindle-shaped, spreading with long filopodia, spreading with lamellipodia, and fully spread. Adherent normal rat platelets displayed all stages of spreading within 30 seconds to 2 minutes, including many spindle-shaped forms, and forms with multiple, long filopodia. In contrast, adhered WF platelets at these early time points rarely developed long filopodia or were spindle shaped. The majority of adherent WF platelets at these early time points were either round, spread with a few short filopodia, or extensively spread with wide lamellipodial skirts. By 15 to 30 minutes, most platelets in both Wistar and WF samples were fully spread. These data show abnormal WF platelet spreading. The paucity of spindle-shaped forms and forms with long filopodia may reflect an inability of WF platelets to undergo the early stages of spreading, or, alternatively, their more rapid than normal progression through these stages. We hypothesize that this failure to spread normally may relate to prolonged bleeding times in vivo and defective clot formation in WF rats.
authors.affiliation: Department of Pathology, Oregon Health Sciences University, Portland, OR 97201, USA.
authors.forename: P E
authors.initials: PE
authors.lastname: Stenberg
authors.forename: R J
authors.initials: RJ
authors.lastname: Barrie
authors.forename: T I
authors.initials: TI
authors.lastname: Pestina
authors.forename: S A
authors.initials: SA
authors.lastname: Steward
authors.forename: J T
authors.initials: JT
authors.lastname: Arnold
authors.forename: A K
authors.initials: AK
authors.lastname: Murti
authors.forename: N K
authors.initials: NK
authors.lastname: Hutson
authors.forename: C W
authors.initials: CW
authors.lastname: Jackson
mesh_terms: D000818:Animals; D001760:Bleeding Time; D001778:Blood Coagulation Disorders / Q000097:blood / Q000235:genetics / Q000473:pathology*; D001792:Blood Platelets / Q000502:physiology* / Q000648:ultrastructure*; D005455:Fluorescent Antibody Technique; D005898:Glass; D007700:Kinetics; D008297:Male; D008855:Microscopy, Electron, Scanning; D008863:Microspheres; D010973:Platelet Adhesiveness; D011107:Polylysine; D051381:Rats; D011920:Rats, Inbred WF; D017208:Rats, Wistar; D013921:Thrombocytopenia / Q000235:genetics / Q000473:pathology
chemical_list: D011107:Polylysine