title: Overexpression of neurogenin induces ectopic expression of HuC in zebrafish.
abstract: Several basic helix-loop-helix (bHLH) transcription factors are known to be involved in vertebrate neurogenesis. To investigate their roles in zebrafish neurogenesis, we isolated cDNAs for homologues of neurogenin and Math(-1)/atonal. The transcription of neurogenin was first detectable in zebrafish nervous system at late gastrulation stage. The expression of zebrafish neurogenin precedes and overlaps that of HuC, one of the earliest neuronal precursor markers. Injection of neurogenin mRNA into early stage zebrafish embryos induced ectopic expression of HuC. These results suggest that neurogenin may participate in the generation of HuC-expressing cells, implying its role in neuronal determination in zebrafish.
authors.affiliation: Division of Molecular Oncology, Biomedical Research Center, Osaka University School of Medicine, Japan.
authors.forename: C H
authors.initials: CH
authors.lastname: Kim
authors.forename: Y K
authors.initials: YK
authors.lastname: Bae
authors.forename: Y
authors.initials: Y
authors.lastname: Yamanaka
authors.forename: S
authors.initials: S
authors.lastname: Yamashita
authors.forename: T
authors.initials: T
authors.lastname: Shimizu
authors.forename: R
authors.initials: R
authors.lastname: Fujii
authors.forename: H C
authors.initials: HC
authors.lastname: Park
authors.forename: S Y
authors.initials: SY
authors.lastname: Yeo
authors.forename: T L
authors.initials: TL
authors.lastname: Huh
authors.forename: M
authors.initials: M
authors.lastname: Hibi
authors.forename: T
authors.initials: T
authors.lastname: Hirano
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D000954:Antigens, Surface / Q000378:metabolism*; D051792:Basic Helix-Loop-Helix Proteins; D015415:Biomarkers / Q000032:analysis; D001921:Brain / Q000196:embryology / Q000378:metabolism*; D051959:ELAV Proteins; D000067778:ELAV-Like Protein 3; D018507:Gene Expression Regulation, Developmental; D017403:In Situ Hybridization; D008969:Molecular Sequence Data; D009419:Nerve Tissue Proteins / Q000378:metabolism* / Q000502:physiology*; D009420:Nervous System / Q000196:embryology / Q000378:metabolism*; D012333:RNA, Messenger / Q000032:analysis / Q000235:genetics; D016601:RNA-Binding Proteins; D029867:Xenopus Proteins; D015027:Zebrafish / Q000196:embryology*; D029961:Zebrafish Proteins
chemical_list: D000954:Antigens, Surface; D051792:Basic Helix-Loop-Helix Proteins; D015415:Biomarkers; D051959:ELAV Proteins; D000067778:ELAV-Like Protein 3; D009419:Nerve Tissue Proteins; D012333:RNA, Messenger; D016601:RNA-Binding Proteins; D029867:Xenopus Proteins; D029961:Zebrafish Proteins; C088635:elavl3 protein, zebrafish; C101907:neurogenin, Xenopus