Elutability of Proteins from Aluminum-Containing Vaccine Adjuvants by Treatment with Surfactants
The elutability of proteins from adjuvants in model vaccines composed of ovalbumin adsorbed by aluminum hydroxide adjuvant or lysozyme adsorbed by aluminum phosphate adjuvant following treatment with surfactant solutions was studied. Nonionic (Triton X-100, lauryl maltoside), zwitterionic (lauryl sulfobetaine), anionic (sodium dodecyl sulfate), and cationic (cetylpyridinium chloride, dodecyltrimethylammonium chloride) surfactants were investigated. Cetylpyridinium chloride produced the greatest degree of elution (60%) of ovalbumin from aluminum hydroxide adjuvant. Sodium dodecyl sulfate completely eluted lysozyme from aluminum phosphate adjuvant. The effectiveness of surfactants in removing preadsorbed proteins was directly related to their ability to denature the protein. Micellar solubilization and electrostatic repulsion may also contribute to desorption. Copyright 1998 Academic Press. Copyright 1998Academic Press
Rinella
JV
Department of Industrial and Physical Pharmacy, Purdue University, West Lafayette, Indiana, 47907
Workman
RF
Hermodson
MA
White
JL
Hem
SL