title: Fertilization and development of mouse oocytes injected with membrane-damaged spermatozoa.
abstract: The objective of this study was to investigate the influence of sperm plasma membrane on fertilization and development in a mouse model. The sperm plasma membrane was destroyed by exposure to Triton X-100 prior to intracytoplasmic sperm injection (ICSI). A single sperm curling (SSC) test was used to evaluate cell viability. The fertilization rates of oocytes obtained following ICSI of membrane-damaged sperm was not significantly higher than that of the control group (62.4 versus 59.6%). Rates of development to blastocyst were also not significantly different (51.7 and 50%). Inner cell mass (ICM) and total embryo cell numbers in the two groups were not statistically different (16 +/- 3.7 versus 14.73 +/- 3.35 and 45.8 +/- 12.5 versus 39.5 +/- 12 respectively). There were no differences in the implantation and live fetus rates between the two groups after transfer to pseudopregnant mice (61.5 and 35.9% respectively for the membrane-damaged group and 53.5 and 31.4% for the intact group). In conclusion, the present study clearly shows that destruction of the sperm plasma membrane does not affect fertilization and further development following injection of membrane-damaged spermatozoa into mouse oocytes. Fertilization and development can be achieved by dead spermatozoa at an early stage of necrosis when only the plasma membrane has been damaged.
authors.affiliation: Department of Obstetrics and Gynaecology, National University of Singapore, Singapore.
authors.forename: A
authors.initials: A
authors.lastname: Ahmadi
authors.forename: S C
authors.initials: SC
authors.lastname: Ng
mesh_terms: D000818:Animals; D001755:Blastocyst / Q000502:physiology; D002462:Cell Membrane / Q000187:drug effects / Q000502:physiology*; D002970:Cleavage Stage, Ovum; D010064:Embryo Implantation; D004624:Embryo Transfer; D005260:Female; D005307:Fertilization in Vitro; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D008808:Mice, Inbred CBA; D008845:Microinjections; D017830:Octoxynol / Q000494:pharmacology; D009865:Oocytes / Q000502:physiology*; D011247:Pregnancy; D011256:Pregnancy Outcome; D013094:Spermatozoa / Q000502:physiology / Q000648:ultrastructure*
chemical_list: D017830:Octoxynol