Paracoccidioides cerebriformis Moore, 1935. Mycologic and immunochemical study.
The present study concern on mycologic and immunochemical data obtained from two samples of a fungus considered as belonging to the species Paracoccidioides cerebriformis described by Moore in 1935, and maintained since then on Sabouraud's agar in the mycology collection of the Instituto de Medicina Tropical de São Paulo. After 60 years, the samples exhibited the same characteristics described by MOORE (1935). However, experimental lesions did not resulted in guinea-pigs inoculated intratesticularly. The dominant antigen in Paracoccidioides brasiliensis, 43 kDa glicoprotein (gp43), could not be demonstrated by SDS PAGE and Western blotting. Immunoelectrophoresis did not demonstrated the E arch of cathodic migration using a policlonal anti gp43 serum. According to these findings, it is concluded that the fungus described by MOORE (1935) as P. cerebriformis does not belong to the genus Paracoccidioides. Paracoccidioidomycosis should therefore be considered as resulting from infection by a single species, Paracoccidioides brasiliensis (Splendore, 1912) as asserted by ALMEIDA (1930). Further studies, through molecular biology methods, could identify the mentioned fungus.
Lacaz
C da S
Cda S
Barnes Hospital, St. Louis, Missouri, USA.
Vidal
M S
MS
Pereira
C N
CN
Heins-Vaccari
E M
EM
de Melo
N T
NT
Sakai-Valente
N
N
Arriagada
G L
GL
D000818:Animals; D006168:Guinea Pigs; D006801:Humans; D007150:Immunohistochemistry; D010228:Paracoccidioides / Q000145:classification* / Q000276:immunology*