title: Mucosal chemokine activity in Helicobacter pylori infection.
abstract: We examined secretion, mRNA expression, and histologic localization of interleukin-8 (IL-*) and growth-related gene product-alpha (GRO alpha) in the Helicobacter pylori-infected gastric antral mucosa. Antral biopsies were obtained from an area of endoscopically intact mucosa. Significantly higher levels of IL-8 and GRO alpha were secreted in organ cultures from patients with H. pylori infection, and their elevation was prominent in patients with duodenal ulcer. There was a significant association between these alpha-chemokine levels and histologic grades of activity, inflammation, and H. pylori density. In fresh antral biopsies, IL-8 and GRO alpha mRNA expression was detected more frequently in H. pylori-infected patients compared with those without infection. Immunofluorescence microscopy showed localization of IL-8 and GRO alpha proteins in gastric epithelial cells and infiltrating CD68+ macrophages. In the chemotaxis assay, a significant positive correlation was found between neutrophil migration induced by the organ culture supernatants and their contents of IL-8 and GRO alpha. After H. pylori eradication, a significant decrease was observed in IL-8 and GRO alpha levels detected in organ cultures. In conclusion, mucosal alpha-chemokine activity correlates well with histologic severity of H. pylori-associated antral gastritis and can be used to predict the effects of H. pylori eradication therapy.
authors.affiliation: First Department of Internal Medicine, Nagoya University School of Medicine, Japan.
authors.forename: K
authors.initials: K
authors.lastname: Kusugami
authors.forename: T
authors.initials: T
authors.lastname: Ando
authors.forename: M
authors.initials: M
authors.lastname: Ohsuga
authors.forename: A
authors.initials: A
authors.lastname: Imada
authors.forename: M
authors.initials: M
authors.lastname: Shinoda
authors.forename: T
authors.initials: T
authors.lastname: Konagaya
authors.forename: K
authors.initials: K
authors.lastname: Ina
authors.forename: N
authors.initials: N
authors.lastname: Kasuga
authors.forename: A
authors.initials: A
authors.lastname: Fukatsu
authors.forename: S
authors.initials: S
authors.lastname: Ichiyama
authors.forename: T
authors.initials: T
authors.lastname: Nada
authors.forename: M
authors.initials: M
authors.lastname: Ohta
mesh_terms: D000328:Adult; D001706:Biopsy; D054360:Chemokine CXCL1; D018925:Chemokines / Q000096:biosynthesis*; D019743:Chemokines, CXC; D002630:Chemotactic Factors / Q000096:biosynthesis*; D002634:Chemotaxis, Leukocyte; D005260:Female; D019084:Fluorescent Antibody Technique, Indirect; D005753:Gastric Mucosa / Q000378:metabolism* / Q000382:microbiology; D005756:Gastritis / Q000378:metabolism / Q000382:microbiology*; D015870:Gene Expression; D006133:Growth Substances / Q000096:biosynthesis*; D016481:Helicobacter Infections / Q000378:metabolism*; D016480:Helicobacter pylori; D006801:Humans; D036341:Intercellular Signaling Peptides and Proteins; D016209:Interleukin-8 / Q000096:biosynthesis*; D008297:Male; D008875:Middle Aged; D009504:Neutrophils / Q000502:physiology; D010437:Peptic Ulcer / Q000378:metabolism / Q000382:microbiology*; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000235:genetics
chemical_list: C475829:CXCL1 protein, human; D054360:Chemokine CXCL1; D018925:Chemokines; D019743:Chemokines, CXC; D002630:Chemotactic Factors; D006133:Growth Substances; D036341:Intercellular Signaling Peptides and Proteins; D016209:Interleukin-8; D012333:RNA, Messenger