New curcuminoids isolated from Zingiber cassumunar protect cells suffering from oxidative stress: a flow-cytometric study using rat thymocytes and H2O2.
Effects of new complex curcuminoids (cassumunin A and cassumunin B) isolated from tropical ginger, Zingiber cassumunar, were examined in dissociated rat thymocytes suffering from oxidative stress induced by 3 mM hydrogen peroxide by using a flow cytometer and ethidium bromide. The effects were compared with those of curcumin, a natural antioxidant, whose chemical structure is included in those of cassumunins A and B. Pretreatment of rat thymocytes with the respective cassumunins at concentrations ranging from 100 nM to 3 microM dose-dependently prevented the hydrogen peroxide (H2O2)-induced decrease in cell viability. It had the same action, although less effective, against the treatment with cassumunin A or B (3 microM) immediately after or 60 min after start of the oxidative stress. Respective potencies of cassumunins A and B in protecting the cells suffering from H2O2-induced oxidative stress were greater than that of curcumin. It is suggested that cassumunins A and B may possess a potent protective action on living cells suffering from oxidative stress.
Nagano
T
T
Laboratory of Cellular Signaling, Faculty of Integrated Arts and Sciences, The University of Tokushima, Japan.
Oyama
Y
Y
Kajita
N
N
Chikahisa
L
L
Nakata
M
M
Okazaki
E
E
Masuda
T
T
D000818:Animals; D000975:Antioxidants / Q000494:pharmacology*; D001393:Azoles / Q000494:pharmacology; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D003474:Curcumin / Q000031:analogs & derivatives* / Q000302:isolation & purification / Q000494:pharmacology*; D003676:Deferoxamine / Q000494:pharmacology; D004121:Dimethyl Sulfoxide / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D004996:Ethidium; D005434:Flow Cytometry; D006861:Hydrogen Peroxide / Q000037:antagonists & inhibitors; D054833:Isoindoles; D016566:Organoselenium Compounds / Q000494:pharmacology; D018384:Oxidative Stress / Q000187:drug effects*; D051381:Rats; D017208:Rats, Wistar; D013950:Thymus Gland / Q000187:drug effects*; D019862:Zingiberales / Q000737:chemistry*
D000975:Antioxidants; D001393:Azoles; D054833:Isoindoles; D016566:Organoselenium Compounds; C110984:cassumunin B; C042986:ebselen; D006861:Hydrogen Peroxide; D004996:Ethidium; D003474:Curcumin; D003676:Deferoxamine; D004121:Dimethyl Sulfoxide