Determining sodium azide concentration in blood by ion chromatography.
We describe a simple method for measuring sodium azide concentrations in aliquots of blood and other tissues. Aliquots are acidified, converting azide to volatile hydrazoic acid (HN3) which is then trapped in sodium hydroxide. We analyze the resulting aliquots by ion chromatography, using a sodium tetraborate eluent and suppressed conductivity detection. The method is sensitive to at least 100 ng/mL.
Kruszyna
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R
Department of Pharmacology and Toxicology, Dartmouth Medical School, Hanover, New Hampshire, USA.
Smith
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RP
Kruszyna
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D001386:Azides / Q000032:analysis; D001881:Borates; D002852:Chromatography, Ion Exchange / Q000379:methods*; D006801:Humans; D012680:Sensitivity and Specificity; D019810:Sodium Azide / Q000032:analysis / Q000097:blood*; D012972:Sodium Hydroxide
D001386:Azides; D001881:Borates; D012972:Sodium Hydroxide; C007972:hydrazoic acid; C010634:sodium borate; D019810:Sodium Azide