Identification of the TYKY homologous subunit of complex I from Neurospora crassa.
A polypeptide subunit of complex I from Neurospora crassa, homologous to bovine TYKY, was expressed in Escherichia coli, purified and used for the production of rabbit antiserum. The mature mitochondrial protein displays a molecular mass of 21280 Da and results from cleavage of a presequence consisting of the first 34 N-terminal amino acids of the precursor. This protein was found closely associated with the peripheral arm of complex I.
Duarte
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Instituto de Ciências Biomédicas de Abel Salazar, Universidade do Porto, Portugal.
Schulte
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Videira
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D000818:Animals; D000908:Antibodies, Fungal; D002417:Cattle; D004926:Escherichia coli / Q000235:genetics; D007506:Iron-Sulfur Proteins / Q000032:analysis / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D008928:Mitochondria / Q000737:chemistry; D008970:Molecular Weight; D016660:NAD(P)H Dehydrogenase (Quinone) / Q000032:analysis / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D009492:Neurospora crassa / Q000201:enzymology* / Q000235:genetics / Q000276:immunology; D011485:Protein Binding; D011498:Protein Precursors; D011817:Rabbits; D017386:Sequence Homology, Amino Acid
D000908:Antibodies, Fungal; D007506:Iron-Sulfur Proteins; D011498:Protein Precursors; D016660:NAD(P)H Dehydrogenase (Quinone)