title: Effects of ventilation style on surfactant metabolism and treatment response in preterm lambs.
abstract: We investigated whether the style of ventilation would influence respiratory physiology or surfactant metabolism in surfactant-treated preterm lambs. Preterm lambs were delivered at 131 +/- 1 d gestation and treated with an organic solvent extract of sheep surfactant (100 mg/kg). The lambs were randomized to ventilation peiods of 2 h, 5 h, 10 h, or 24 h, and to ventilation with a low rate (15 breaths/min) and high VT (15 ml/kg), with a high rate (50 breaths/min) and low VT (8 ml/kg), or with high-frequency oscillatory ventilation (HFOV). Gas exchange and lung volumes were similar across time and for the different ventilation styles. Saturated phosphatidylcholine (SatPC) in alveolar lavage was lower for the HFOV group than for the other ventilation groups at 10 h and 24 h. The rate of loss of surfactant protein B (SP-B) from these preterm animals' lungs was slow and not influenced by ventilation style. The percentages of surfactants in large-aggregate forms were not changed by style of ventilation, and the large-aggregate surfactants had excellent function when tested in surfactant-deficient preterm rabbits. Alveolar lavage protein was low (30 ml/kg), and tissue hyaluronan did not change with time or ventilation style. In preterm lambs ventilated without causing injury, the extreme styles of ventilation examined in the study had minimal effects on lung function, surfactant function, or surfactant metabolism.
authors.affiliation: Department of Pediatrics, Harbor UCLA Medical Center, Torrance, California, USA.
authors.forename: M
authors.initials: M
authors.lastname: Ikegami
authors.forename: K
authors.initials: K
authors.lastname: Wada
authors.forename: G A
authors.initials: GA
authors.lastname: Emerson
authors.forename: C M
authors.initials: CM
authors.lastname: Rebello
authors.forename: R E
authors.initials: RE
authors.lastname: Hernandez
authors.forename: A H
authors.initials: AH
authors.lastname: Jobe
mesh_terms: D000818:Animals; D000831:Animals, Newborn / Q000502:physiology*; D005865:Gestational Age; D011510:Proteolipids / Q000493:pharmacokinetics; D011663:Pulmonary Surfactants / Q000378:metabolism* / Q000493:pharmacokinetics / Q000494:pharmacology* / Q000502:physiology; D011817:Rabbits; D012119:Respiration / Q000502:physiology*; D012756:Sheep
chemical_list: D011510:Proteolipids; D011663:Pulmonary Surfactants