title: Genome structure of cucumber leaf spot virus: sequence analysis suggests it belongs to a distinct species within the Tombusviridae.
abstract: The complete nucleotide sequence of cucumber leaf spot virus (CLSV) has been determined and the sizes and locations of predicted viral proteins deduced. The genome consists of 4432 nucleotides and contains five long ORFs. The 5' proximal ORF encodes a 25 kDa product that terminates in an amber codon which may be readthrough to produce an 84 kDa protein (ORF 2). ORF 3 codes for the 41 kDa coat protein (CP). ORFs 4 and 5 are completely overlapping at the 3' terminus and code for 27 and 17 kDa products, respectively. The CLSV genome structure is similar to that of tombusviruses and nearly identical to pothos latent virus (PoLV), a newly proposed, atypical, member of the Tombusviridae. It is proposed that CLSV and PoLV be considered strains of a new tombusvirus species. Amino acid sequence comparisons of the CLSV CP and the CPs of several small spherical plant viruses suggest that CLSV is most closely related to melon necrotic spot carmovirus (MNSV), red clover necrotic mosaic dianthovirus (RCNMV) and cucumber necrosis tombusvirus (CNV). These viruses, like CLSV, are transmitted by the soil inhabiting fungus, Olpidium bornovanus.
authors.affiliation: Agriculture and Agri-Food Canada, Pacific Agri-Food Research Centre, Summerland, Canada.
authors.forename: J S
authors.initials: JS
authors.lastname: Miller
authors.forename: H
authors.initials: H
authors.lastname: Damude
authors.forename: M A
authors.initials: MA
authors.lastname: Robbins
authors.forename: R D
authors.initials: RD
authors.lastname: Reade
authors.forename: D M
authors.initials: DM
authors.lastname: Rochon
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D018553:Cucumis sativus / Q000821:virology; D015967:Gene Expression Regulation, Viral; D016679:Genome, Viral; D008969:Molecular Sequence Data; D010942:Plant Viruses / Q000145:classification / Q000235:genetics; D012333:RNA, Messenger / Q000032:analysis; D012367:RNA, Viral / Q000032:analysis; D017422:Sequence Analysis, DNA; D019183:Tombusviridae / Q000145:classification / Q000235:genetics*
chemical_list: D012333:RNA, Messenger; D012367:RNA, Viral