Peculiarities of gene expression of the EcoRII modification-restriction system.
The restriction-modification genes of the EcoRII system have been cloned into plasmids under control of phage-specific promoters T7 and SP6. The transcription was induced by cell infection with the recombinant M13 phages with the corresponding genes of phage RNA-polymerases under control of the Plac-promoter in the presence of IPTG. The induction yields significant amounts of EcoRII DNA-methylase for both phage-specific promoters. In both cases no increase in EcoRII endonuclease expression could be achieved. We hypothesize that the expression of the endonuclease gene is regulated on the translational level.
Matvienko
N N
NN
Branch of Shemyakin-Ovchinnikov, Russian Academy of Sciences, Pushchino, Moscow Region, Russia.
Zheleznaya
L A
LA
Chernyshova
E E
EE
Buryanov YaI
Matvienko
N I
NI
D017104:Bacteriophage M13 / Q000235:genetics; D003001:Cloning, Molecular; D004268:DNA-Binding Proteins / Q000235:genetics; D015257:DNA-Cytosine Methylases / Q000235:genetics / Q000378:metabolism; D015252:Deoxyribonucleases, Type II Site-Specific / Q000235:genetics* / Q000378:metabolism; D004591:Electrophoresis, Polyacrylamide Gel; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism; D005786:Gene Expression Regulation / Q000235:genetics*; D007544:Isopropyl Thiogalactoside / Q000494:pharmacology; D007763:Lac Operon; D010957:Plasmids; D011401:Promoter Regions, Genetic / Q000235:genetics; D014176:Protein Biosynthesis / Q000235:genetics
D004268:DNA-Binding Proteins; D007544:Isopropyl Thiogalactoside; C029528:DNA modification methylase EcoRII; D015257:DNA-Cytosine Methylases; C046545:CCWGG-specific type II deoxyribonucleases; D015252:Deoxyribonucleases, Type II Site-Specific