Structure and function of a sea urchin orthodenticle-related gene (HpOtx).
Two distinct types of orthodenticle-related proteins (HpOtxE/L) have been implicated as transcription activators of the aboral ectoderm-specific arylsulfatase (Ars) gene. Here, we describe the structure of HpOtx gene and present evidence that mRNAs of HpOtxE/L are transcribed from a single HpOtx gene by altering the transcription start site and by alternative splicing. By transactivation experiments, we have also demonstrated that HpOtxL activates the Ars promoter in the gastrula-stage embryo.
Kiyama
T
T
Faculty of Science, Hiroshima University, Kagamiyama, Japan.
Akasaka
K
K
Takata
K
K
Mitsunaga-Nakatsubo
K
K
Sakamoto
N
N
Shimada
H
H
D000818:Animals; D001192:Arylsulfatases / Q000235:genetics; D001483:Base Sequence; D018387:Codon, Initiator; D029721:Drosophila Proteins; D004789:Enzyme Activation / Q000235:genetics; D005786:Gene Expression Regulation; D005801:Genes, Homeobox; D018398:Homeodomain Proteins / Q000737:chemistry / Q000235:genetics* / Q000502:physiology*; D008156:Luciferases / Q000235:genetics / Q000378:metabolism; D008969:Molecular Sequence Data; D011401:Promoter Regions, Genetic; D017434:Protein Structure, Tertiary; D011993:Recombinant Fusion Proteins / Q000502:physiology; D012617:Sea Urchins / Q000235:genetics*; D017422:Sequence Analysis, DNA; D014158:Transcription, Genetic
D018387:Codon, Initiator; D029721:Drosophila Proteins; D018398:Homeodomain Proteins; D011993:Recombinant Fusion Proteins; C093053:oc protein, Drosophila; D008156:Luciferases; D001192:Arylsulfatases