IA induction by gut and epidermal cells of nude mice following administration of human lymphocytes.
Expression of class II molecules by epidermal cells of BALB/c nude mice following an adoptive transfer of lymphoid cells from normal semisyngeneic mice has been previously observed. The purpose of the present study was to determine whether similar expression might be observed after injection of human lymphocytes and to utilize this model to determine whether there would be expression of class II molecules by mucosal and epidermal cells following administration of lymphocytes from young and old donors. In order to address this question, a group of nude mice received a single i.v. injection of human T lymphocytes obtained from aged subjects, and a second group received i.v. injection of lymphocytes from young volunteers. Evaluation of I-A and I-E molecules expression of murine cells were performed by indirect immunoperoxidase staining. More prominent and diffuse staining of class II was observed in the mucosal cells as compared with the keratinocytes. Diffuse and intense staining of intestinal cells was observed mainly in mice treated with lymphocytes from young donors.
Gilhar
A
A
Skin Research Laboratory, Bruce Rappaport Faculty of Medicine, Technion-Israel Institute of Technology, Haifa, Israel.
Michalak
R
R
Sneer
A
A
D019264:Adoptive Transfer; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000818:Animals; D016922:Cellular Senescence / Q000276:immunology; D000949:Histocompatibility Antigens Class II / Q000032:analysis*; D006801:Humans; D007413:Intestinal Mucosa / Q000166:cytology / Q000276:immunology*; D007421:Intestine, Small / Q000166:cytology / Q000276:immunology*; D015603:Keratinocytes / Q000276:immunology*; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude / Q000276:immunology*; D008875:Middle Aged; D013047:Specific Pathogen-Free Organisms; D013601:T-Lymphocytes / Q000276:immunology*
D000949:Histocompatibility Antigens Class II