title: Preparation and characterization of everted membrane vesicles from cells of Staphylococcus aureus.
abstract: We developed a method for the preparation of everted membrane vesicles from cells of Staphylococcus aureus. The cells were first treated with ampicillin to weaken the peptidoglycan layer, then the cells were passed through a French press cell. The resulting vesicles were roughly 0.1 microm in diameter, judging from electron microscopic observations. We detected fairly high membrane-bound ATPase activity in the membrane vesicles. We observed respiratory-driven quenching of quinacrine fluorescence, which indicates that inward H+ transport took place. These results indicate that the vesicles are everted. We characterized the membrane-bound ATPase. We also detected Na+/H+ antiport, erythromycin/H+ antiport and chloramphenicol/H+ antiport activities in the membranes of S. aureus.
authors.affiliation: Department of Microbiology, Faculty of Pharmaceutical Sciences, Okayama University, Tsushima, Japan.
authors.forename: K
authors.initials: K
authors.lastname: Kodama
authors.forename: A
authors.initials: A
authors.lastname: Hashimoto
authors.forename: Y
authors.initials: Y
authors.lastname: Morita
authors.forename: K
authors.initials: K
authors.lastname: Tomochika
authors.forename: T
authors.initials: T
authors.lastname: Tsuchiya
mesh_terms: D000251:Adenosine Triphosphatases / Q000378:metabolism; D000667:Ampicillin / Q000494:pharmacology; D017920:Antiporters / Q000378:metabolism; D001426:Bacterial Proteins / Q000096:biosynthesis; D002462:Cell Membrane / Q000737:chemistry / Q000187:drug effects / Q000201:enzymology; D004734:Energy Metabolism / Q000187:drug effects / Q000502:physiology; D005453:Fluorescence; D006863:Hydrogen-Ion Concentration; D008854:Microscopy, Electron; D010406:Penicillins / Q000494:pharmacology; D013211:Staphylococcus aureus / Q000737:chemistry* / Q000201:enzymology / Q000648:ultrastructure
chemical_list: D017920:Antiporters; D001426:Bacterial Proteins; D010406:Penicillins; D000667:Ampicillin; D000251:Adenosine Triphosphatases