Polyoma middle T antigen in HL-60 cells accelerates hematopoietic myeloid and monocytic cell differentiation.
Expression of the polyoma virus middle T antigen in HL-60 cells accelerates their differentiation in response to both monocytic and granulocytic differentiation-inducing agents. Middle T-expressing cells treated with the granulocytic inducer retinoic acid or the monocytic inducer 1,25-dihydroxy vitamin D3 differentiated 24 h earlier than parental, mock-electroporated, or vector control cell lines. The rapid onset of differentiation correlated with an increase in the cellular level of the middle T protein as well as two known retinoic-acid-inducible markers in HL-60 cells: the paxillin and transglutaminase gene products. The accelerated functional differentiation response and expression of retinoic-acid-inducible markers indicate that middle T played a causal role in differentiation. Thus, expression of the polyoma middle T antigen in HL-60 cells enhanced a variety of molecular changes associated with cellular differentiation.
Platko
J D
JD
Department of Pathology, College of Veterinary Medicine, Cornell University, Ithaca, New York 14850, USA.
Forbes
M E
ME
Varvayanis
S
S
Williams
M N
MN
Brooks
S C
SC
Cherington
V
V
Yen
A
A
D000952:Antigens, Polyomavirus Transforming / Q000096:biosynthesis*; D002117:Calcitriol / Q000494:pharmacology; D015815:Cell Adhesion Molecules / Q000096:biosynthesis; D002454:Cell Differentiation / Q000187:drug effects / Q000502:physiology*; D002455:Cell Division / Q000187:drug effects; D003598:Cytoskeletal Proteins / Q000096:biosynthesis; D006098:Granulocytes / Q000166:cytology*; D018922:HL-60 Cells; D006801:Humans; D007700:Kinetics; D009000:Monocytes / Q000166:cytology*; D051419:Paxillin; D010750:Phosphoproteins / Q000096:biosynthesis; D011994:Recombinant Proteins / Q000096:biosynthesis; D013997:Time Factors; D014162:Transfection; D011503:Transglutaminases / Q000096:biosynthesis; D014212:Tretinoin / Q000494:pharmacology
D000952:Antigens, Polyomavirus Transforming; D015815:Cell Adhesion Molecules; D003598:Cytoskeletal Proteins; C496061:PXN protein, human; D051419:Paxillin; D010750:Phosphoproteins; D011994:Recombinant Proteins; D014212:Tretinoin; D011503:Transglutaminases; D002117:Calcitriol