title: The transcription factor NF-ATc1 regulates lymphocyte proliferation and Th2 cytokine production.
abstract: NF-ATc1 is a member of a family of genes that encodes the cytoplasmic component of the nuclear factor of activated T cells (NF-AT). In activated T cells, nuclear NF-AT binds to the promoter regions of multiple cytokine genes and induces their transcription. The role of NF-ATc1 was investigated in recombination activating gene-1 (RAG-1)-deficient blastocyst complementation assays using homozygous NF-ATc1-/- mutant ES cell lines. NF-ATc1-/-/RAG-1-/- chimeric mice showed reduced numbers of thymocytes and impaired proliferation of peripheral lymphocytes, but normal production of IL-2. Induction in vitro of Th2 responses, as demonstrated by a decrease in IL-4 and IL-6 production, was impaired in mutant T cells. These data indicate that NF-ATc1 plays roles in the development of T lymphocytes and in the differentiation of the Th2 response.
authors.affiliation: The Amgen Institute, Department of Medical Biophysics, University of Toronto, Ontario, Canada.
authors.forename: H
authors.initials: H
authors.lastname: Yoshida
authors.forename: H
authors.initials: H
authors.lastname: Nishina
authors.forename: H
authors.initials: H
authors.lastname: Takimoto
authors.forename: L E
authors.initials: LE
authors.lastname: Marengère
authors.forename: A C
authors.initials: AC
authors.lastname: Wakeham
authors.forename: D
authors.initials: D
authors.lastname: Bouchard
authors.forename: Y Y
authors.initials: YY
authors.lastname: Kong
authors.forename: T
authors.initials: T
authors.lastname: Ohteki
authors.forename: A
authors.initials: A
authors.lastname: Shahinian
authors.forename: M
authors.initials: M
authors.lastname: Bachmann
authors.forename: P S
authors.initials: PS
authors.lastname: Ohashi
authors.forename: J M
authors.initials: JM
authors.lastname: Penninger
authors.forename: G R
authors.initials: GR
authors.lastname: Crabtree
authors.forename: T W
authors.initials: TW
authors.lastname: Mak
mesh_terms: D000818:Animals; D002454:Cell Differentiation / Q000502:physiology; D002460:Cell Line; D002678:Chimera; D016207:Cytokines / Q000096:biosynthesis*; D004268:DNA-Binding Proteins / Q000235:genetics / Q000276:immunology / Q000502:physiology*; D018398:Homeodomain Proteins; D008213:Lymphocyte Activation / Q000276:immunology / Q000502:physiology*; D051379:Mice; D050778:NFATC Transcription Factors; D009687:Nuclear Proteins; D013234:Stem Cells / Q000166:cytology / Q000378:metabolism; D013601:T-Lymphocytes / Q000166:cytology / Q000276:immunology; D018418:Th2 Cells / Q000276:immunology / Q000378:metabolism*; D013950:Thymus Gland / Q000166:cytology / Q000276:immunology / Q000502:physiology; D014157:Transcription Factors / Q000276:immunology / Q000502:physiology*
chemical_list: D016207:Cytokines; D004268:DNA-Binding Proteins; D018398:Homeodomain Proteins; D050778:NFATC Transcription Factors; D009687:Nuclear Proteins; D014157:Transcription Factors; C064658:RAG-1 protein