DNA-chlorpheniramine interaction studied by spectroscopic techniques.
It was previously studied that the antihistaminic chlorpheniramine elicits a biphasic response on cell growth and regulates polyamine metabolism, as described for polyamines. In part, polyamine effects on macromolecular synthesis and cell growth are attributed to nucleic acid:polyamine interactions. In this work, we have tested the hypothesis of a DNA:chlorpheniramine interaction, using fluorometry, FTIR and Raman spectroscopic techniques. The results indicate that DNA:chlorpheniramine interaction occurs inducing conformational changes in the macromolecule by affecting both phosphodiester bonds and bases. Results open new perspectives for characterization of action mechanisms of natural or synthetic diamines with pharmacological or physiological importance.
Medina
M A
MA
Departamento de Bioquímica y Biología Molecular, Facultad de Ciencias, Universidad de Málaga, Spain.
Ramírez
F J
FJ
Ruiz-Chica
J
J
Chavarría
T
T
López-Navarrete
J T
JT
Sánchez-Jiménez
F
F
D002744:Chlorpheniramine / Q000378:metabolism* / Q000494:pharmacology; D004247:DNA / Q000737:chemistry / Q000378:metabolism*; D003959:Diamines / Q000378:metabolism; D004587:Electrophoresis, Agar Gel; D004996:Ethidium / Q000378:metabolism; D005470:Fluorometry; D006634:Histamine H1 Antagonists / Q000494:pharmacology; D007364:Intercalating Agents / Q000378:metabolism; D009690:Nucleic Acid Conformation; D010957:Plasmids / Q000378:metabolism; D017550:Spectroscopy, Fourier Transform Infrared; D013059:Spectrum Analysis, Raman
D003959:Diamines; D006634:Histamine H1 Antagonists; D007364:Intercalating Agents; D002744:Chlorpheniramine; D004247:DNA; D004996:Ethidium