title: KIF3C, a novel member of the kinesin superfamily: sequence, expression, and mapping to human chromosome 2 at 2p23.
abstract: Kinesins are microtubule-dependent molecular motors involved in intracellular transport and mitosis. Here, we report the cloning, sequencing, mapping, and expression of a novel member of the kinesin superfamily. The sequence of this newly identified human cDNA reveals an open reading frame encoding a putative protein of 792 residues. Based on its high sequence similarity to the kinesin-like molecule KIF3B, we named this protein KIF3C. KIF3C is encoded by transcripts that are distinct from the KIF3B mRNA in human, rat, and mouse and is preferentially expressed in the brain. Fluorescence in situ hybridization reveals that, in the human genome, the KIF3C gene maps to chromosome 2 at 2p23. The sequence of KIF3C predicts an unusually long insertion in the proximity of L11, a region thought to mediate microtubule binding. Taken together, these findings suggest that KIF3C is a novel kinesin-like protein that might be specifically involved in microtubule-based transport in neuronal cells.
authors.affiliation: DIBIT-HSR, Via Olgettina 58, Milan, 20132, Italy.
authors.forename: M
authors.initials: M
authors.lastname: Sardella
authors.forename: F
authors.initials: F
authors.lastname: Navone
authors.forename: M
authors.initials: M
authors.lastname: Rocchi
authors.forename: A
authors.initials: A
authors.lastname: Rubartelli
authors.forename: L
authors.initials: L
authors.lastname: Viggiano
authors.forename: G
authors.initials: G
authors.lastname: Vignali
authors.forename: G G
authors.initials: GG
authors.lastname: Consalez
authors.forename: R
authors.initials: R
authors.lastname: Sitia
authors.forename: A
authors.initials: A
authors.lastname: Cabibbo
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D002874:Chromosome Mapping; D002889:Chromosomes, Human, Pair 2 / Q000235:genetics*; D003001:Cloning, Molecular; D015870:Gene Expression; D006801:Humans; D016547:Kinesins / Q000096:biosynthesis* / Q000235:genetics*; D051379:Mice; D008810:Mice, Inbred C57BL; D008969:Molecular Sequence Data; D051381:Rats; D017386:Sequence Homology, Amino Acid
chemical_list: C110520:KIF3C protein, human; C484676:Kif3c protein, mouse; C484677:Kif3c protein, rat; D016547:Kinesins