Action of chronic irradiation on the cytogenetic damage of human lymphocyte culture.
The action of chronic irradiation (dose rate 2.9 Gy/day) on human lymphocyte culture was investigated. Whole blood was irradiated at 37 degrees C. Aliquots (0.2 ml) of whole blood were cultivated by the standard method. A medium containing phytohemagglutinin was added immediately after irradiation. All structural chromosome- and chromatid-type changes were recorded. The experimental data showed that the conditions of irradiation of lymphocytes affected neither the background level of chromosome damage nor their radiosensitivity. The obtained dose-response curve of chromosome aberrations was described by a linear regression, which then became a plateau. There is no statistically significant difference between the results for the low doses (10-50 cGy) of chronic and acute radiation.
Zaichkina
S I
SI
Laboratory of Cytogenetics, Institute of Theoretical and Experimental Biophysics, Pushchino, Moscow Region, Russia. zaichkina@venus.iteb.serpukhov.su
Aptikaeva
G F
GF
Rozanova
O M
OM
Akhmadieva
A K
AK
Smirnova
E N
EN
Ganassi
E E
EE
D000328:Adult; D002478:Cells, Cultured; D002869:Chromosome Aberrations; D006801:Humans; D008214:Lymphocytes / Q000528:radiation effects* / Q000648:ultrastructure; D008297:Male