Exposure of piglet coronary arterial muscle cells to low concentrations of Mg2+ found in blood of ischemic heart disease patients result in rapid elevation of cytosolic Ca2+: relevance to sudden infant death syndrome.
Exposure of cultured piglet primary neonatal coronary arterial smooth muscle cells to concentrations of ionized Mg2+ ([Mg2+]o (i.e., 0.48, 0.3, 0.15 mM) found in blood of patients presenting with ischemic heart disease and in hypoxic neonates resulted in concentration-dependent elevation in intracellular free Ca2+ ions ([Ca2+]i; the lower the [Mg2+]o, the higher the [Ca2+]i rise. The lowest concentration of [Mg2+]o tested, i.e., 0.15 mM, resulted in a clear rounding-up (i.e., contraction) of many of the coronary smooth muscle cells; reintroduction of normal 1.2 mM [Mg2+]o failed to restore either normal [Ca2+]i or cell shape.
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Department of Physiology, State University of New York Health Science Center at Brooklyn, 11203, USA.
Zhang
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D000818:Animals; D002118:Calcium / Q000378:metabolism*; D002478:Cells, Cultured; D003331:Coronary Vessels / Q000166:cytology / Q000187:drug effects*; D003600:Cytosol / Q000187:drug effects / Q000378:metabolism; D006801:Humans; D007223:Infant; D008275:Magnesium Deficiency / Q000503:physiopathology*; D009131:Muscle, Smooth, Vascular / Q000166:cytology / Q000187:drug effects*; D017202:Myocardial Ischemia / Q000503:physiopathology*; D013398:Sudden Infant Death / Q000209:etiology*; D013552:Swine
D002118:Calcium