title: Characterisation of an outer membrane protein of Moraxella catarrhalis.
abstract: To elucidate potential vaccine antigens, Moraxella catarrhalis outer membrane proteins (OMPs) were studied. We have previously shown an OMP to be a target for human IgG and have now further characterised this OMP which appears to have a molecular mass of 84 kDa and to be distinct from the 81-kDa OMP, CopB. Human transferrin was shown to bind the 84-kDa OMP alone. N-terminal sequencing of this OMP and purified M. catarrhalis transferrin binding protein B (TbpB) revealed homology both with each other and with the TbpB of Haemophilus influenzae and Neisseria meningitidis. Adsorption of human anti-serum with purified TbpB from two M. catarrhalis strains abolished or reduced binding of IgG to the 84-kDa OMP from three M. catarrhalis isolates. IgG binding to CopB was unaffected. It is clear that the 84-kDa OMP is distinct from CopB and is a likely homologue of TbpB.
authors.affiliation: Immunobiology Unit, Institute of Child Health, London, UK.
authors.forename: K E
authors.initials: KE
authors.lastname: Mathers
authors.forename: D
authors.initials: D
authors.lastname: Goldblatt
authors.forename: C
authors.initials: C
authors.lastname: Aebi
authors.forename: R
authors.initials: R
authors.lastname: Yu
authors.forename: A B
authors.initials: AB
authors.lastname: Schryvers
authors.forename: E J
authors.initials: EJ
authors.lastname: Hansen
mesh_terms: D000328:Adult; D001425:Bacterial Outer Membrane Proteins / Q000032:analysis*; D006801:Humans; D008970:Molecular Weight; D001936:Moraxella catarrhalis / Q000737:chemistry*
chemical_list: D001425:Bacterial Outer Membrane Proteins