Thermostable, high salt-tolerant amylase from Bacillus megaterium VUMB-109.
A bacterial strain, Bacillus megaterium VUMB-109, has been isolated and identified which produces salt-tolerant, thermostable amylase (16 U/ml culture filtrate). Cultural conditions such as carbon and nitrogen sources, metal ions, temperature and pH have been optimized for enzyme production. The partially purified enzyme was active over a wide range of pH (4.5-10) and exhibited maximum activity at 95 degrees C, retaining 90% original activity at 100 degrees C. Partially purified enzyme was stable at 70 degrees C for 60 min. The enzyme was stable in NaCl up to 5m over 24 h without losing its original activity.
Jana
M
M
Department of Botany and Forestry, Vidyasagar University, Midnapore, West Bengal, India.
Chattopadhyay
D J
DJ
Pati
B R
BR
D000681:Amylases / Q000096:biosynthesis / Q000187:drug effects / Q000378:metabolism*; D001410:Bacillus megaterium / Q000201:enzymology*; D002244:Carbon; D006361:Heating; D006863:Hydrogen-Ion Concentration; D007477:Ions; D008670:Metals; D009584:Nitrogen; D012965:Sodium Chloride / Q000494:pharmacology*; D013696:Temperature
D007477:Ions; D008670:Metals; D012965:Sodium Chloride; D002244:Carbon; D000681:Amylases; D009584:Nitrogen