A new flavonol glycoside gallate ester from Acer okamotoanum and its inhibitory activity against human immunodeficiency virus-1 (HIV-1) integrase.
Bioassay-directed chromatographic fractionation of an ethyl acetate extract of the leaves of Acer okamotoanum using HIV-1 integrase afforded a new acylated flavonol glycoside, quercetin 3-O-(2",6"-O-digalloyl)-beta-D-galactopyranoside (1), together with six known flavonol glycosides and three known phenolic compounds. The structure of the new compound was determined by spectroscopic methods. The most active compounds were quercetin 3-O-(2"-galloyl)-alpha-L-arabinopyranoside (6) and 1, which exhibited IC50 values of 18.1 +/- 1.3 and 24.2 +/- 6.6 micrograms/mL, respectively, against HIV-1 integrase.
Kim
H J
HJ
Division of Applied Science, Korea Institute of Science & Technology, Seoul, Korea.
Woo
E R
ER
Shin
C G
CG
Park
H
H
D019380:Anti-HIV Agents / Q000302:isolation & purification* / Q000494:pharmacology; D005697:Galactosides / Q000302:isolation & purification* / Q000494:pharmacology; D019428:HIV Integrase Inhibitors / Q000302:isolation & purification* / Q000494:pharmacology*; D015497:HIV-1 / Q000201:enzymology; D006801:Humans; D007723:Korea; D009682:Magnetic Resonance Spectroscopy; D018515:Plant Leaves / Q000737:chemistry; D010946:Plants, Medicinal / Q000737:chemistry*; D011794:Quercetin / Q000031:analogs & derivatives* / Q000302:isolation & purification / Q000494:pharmacology; D016339:Spectrometry, Mass, Fast Atom Bombardment; D013056:Spectrophotometry, Ultraviolet
D019380:Anti-HIV Agents; D005697:Galactosides; D019428:HIV Integrase Inhibitors; C110806:quercetin 3-O-(2'',6''-O-digalloyl)galactopyranoside; D011794:Quercetin