title: Arachidonic-acid-selective cytosolic phospholipase A2 is involved in gastrin-induced AR4-2J-cell proliferation.
abstract: Gastrin/CCK(B) G protein-coupled receptors have been shown to mediate proliferation stimulated by their endogenous ligands. The present study demonstrates the proliferative effect of arachidonic acid on AR4-2J cells. Gastrin induces an [3H]arachidonic-acid release in a dose-dependent manner. The use of a specific inhibitor of cPLA2, AACOCF3 established the involvement of a cPLA2 in the proliferative effect of gastrin. The results also demonstrate that a cytosolic high-molecular-weight PLA2 is activated by gastrin in AR4-2J cells.
authors.affiliation: INSERM U.151, Groupe de Recherche de Biologie et Pathologie Digestive, Institut Louis Bugnard, CHU Rangueil, Toulouse, France. pradayrol@rangueil.inserm.fr
authors.forename: F
authors.initials: F
authors.lastname: Ghrib
authors.forename: S
authors.initials: S
authors.lastname: Pyronnet
authors.forename: M J
authors.initials: MJ
authors.lastname: Bastié
authors.forename: P
authors.initials: P
authors.lastname: Fagot-Revurat
authors.forename: L
authors.initials: L
authors.lastname: Pradayrol
authors.forename: N
authors.initials: N
authors.lastname: Vaysse
mesh_terms: D000818:Animals; D016718:Arachidonic Acid / Q000502:physiology*; D002118:Calcium / Q000378:metabolism; D002455:Cell Division; D002460:Cell Line; D003600:Cytosol / Q000201:enzymology; D004789:Enzyme Activation; D005755:Gastrins / Q000494:pharmacology*; D010741:Phospholipases A / Q000502:physiology*; D054467:Phospholipases A2; D051381:Rats
chemical_list: D005755:Gastrins; D016718:Arachidonic Acid; D010741:Phospholipases A; D054467:Phospholipases A2; D002118:Calcium