title: The box H + ACA snoRNAs carry Cbf5p, the putative rRNA pseudouridine synthase.
abstract: Many or all of the sites of pseudouridine (Psi) formation in eukaryotic rRNA are selected by site-specific base-pairing with members of the box H + ACA class of small nucleolar RNAs (snoRNAs). Database searches previously identified strong homology between the rat nucleolar protein Nap57p, its yeast homolog Cbf5p, and the Escherichia coli Psi synthase truB/P35. We therefore tested whether Cbf5p is required for synthesis of Psi in the yeast rRNA. After genetic depletion of Cbf5p, formation of Psi in the pre-rRNA is dramatically inhibited, resulting in accumulation of the unmodified rRNA. Protein A-tagged Cbf5p coprecipitates all tested members of the box H + ACA snoRNAs but not box C + D snoRNAs or other RNA species. Genetic depletion of Cbf5p leads to depletion of all box H + ACA snoRNAs. These include snR30, which is required for pre-rRNA processing. Depletion of Cbf5p also results in a pre-rRNA processing defect similar to that seen on depletion of snR30. We conclude that Cbf5p is likely to be the rRNA Psi synthase and is an integral component of the box H + ACA class of snoRNPs, which function to target the enzyme to its site of action.
authors.affiliation: Institute of Cell and Molecular Biology, University of Edinburgh, King's Buildings, Edinburgh EH9 3JR, UK.
authors.forename: D L
authors.initials: DL
authors.lastname: Lafontaine
authors.forename: C
authors.initials: C
authors.lastname: Bousquet-Antonelli
authors.forename: Y
authors.initials: Y
authors.lastname: Henry
authors.forename: M
authors.initials: M
authors.lastname: Caizergues-Ferrer
authors.forename: D
authors.initials: D
authors.lastname: Tollervey
mesh_terms: D002466:Cell Nucleolus; D005804:Genes, Lethal; D006836:Hydro-Lyases; D008869:Microtubule-Associated Proteins / Q000235:genetics / Q000378:metabolism*; D009687:Nuclear Proteins / Q000235:genetics; D011485:Protein Binding; D011560:Pseudouridine / Q000096:biosynthesis*; D012322:RNA Precursors / Q000378:metabolism; D012323:RNA Processing, Post-Transcriptional; D012335:RNA, Ribosomal / Q000378:metabolism*; D012342:RNA, Small Nuclear / Q000145:classification / Q000378:metabolism*; D016601:RNA-Binding Proteins / Q000235:genetics / Q000378:metabolism*; D017411:Ribonucleoproteins, Small Nuclear; D012441:Saccharomyces cerevisiae / Q000201:enzymology*; D029701:Saccharomyces cerevisiae Proteins
chemical_list: D008869:Microtubule-Associated Proteins; C107170:NAP57; D009687:Nuclear Proteins; D012322:RNA Precursors; D012335:RNA, Ribosomal; D012342:RNA, Small Nuclear; D016601:RNA-Binding Proteins; D017411:Ribonucleoproteins, Small Nuclear; D029701:Saccharomyces cerevisiae Proteins; D011560:Pseudouridine; D006836:Hydro-Lyases; C082278:CBF5 protein, S cerevisiae