title: GnRH molecular variants in the brain and pituitary gland of pejerrey, Odontesthes bonariensis (Atheriniformes). Immunological and chromatographic evidence for the presence of a novel molecular variant.
abstract: Gonadotropin-releasing hormone (GnRH) molecular variants in the brain and pituitary gland of pejerrey, Odontesthes bonariensis (Atheriniformes), were characterized by gradient reverse phase high performance liquid chromatography (RP-HPLC). Eluted fractions were tested in radioimmunoassays with different antisera. The results show that the brain extract contains three forms of GnRH: one is immunologically and chromatographically similar to cIIGnRH (chicken II), and another is similar to sGnRH (salmon). A third GnRH appears to be chromatographic and immunologically different from the nine other known forms of the vertebrate hormone. This is the only variant present in the pituitary gland.
authors.affiliation: Instituto de Neurociencia (INEUCI-CONICET), Buenos Aires, Argentina.
authors.forename: A V
authors.initials: AV
authors.lastname: Stefano
authors.forename: L F
authors.initials: LF
authors.lastname: Canosa
authors.forename: J L
authors.initials: JL
authors.lastname: D'Eramo
authors.forename: O
authors.initials: O
authors.lastname: Fridman
authors.forename: J M
authors.initials: JM
authors.lastname: Affanni
authors.forename: G M
authors.initials: GM
authors.lastname: Somoza
mesh_terms: D000818:Animals; D001118:Argentina; D001921:Brain / Q000378:metabolism*; D001923:Brain Chemistry; D002851:Chromatography, High Pressure Liquid; D005399:Fishes; D007987:Gonadotropin-Releasing Hormone / Q000031:analogs & derivatives* / Q000032:analysis / Q000737:chemistry / Q000302:isolation & purification* / Q000378:metabolism; D010902:Pituitary Gland / Q000737:chemistry / Q000378:metabolism*; D011498:Protein Precursors / Q000032:analysis / Q000737:chemistry / Q000378:metabolism*; D011863:Radioimmunoassay; D014020:Tissue Extracts / Q000737:chemistry
chemical_list: D011498:Protein Precursors; D014020:Tissue Extracts; C091931:salmon gonadotropin-releasing hormone precursor; D007987:Gonadotropin-Releasing Hormone