Incorporation and release of vancomycin from poly(D,L-lactide-co-glycolide) microspheres.
Spherical monolithic microspheres, with a honeycomb-like internal architecture, composed of PLCG 50:50 and PLCG 75:25 and containing a range of vancomycin loadings, have been fabricated using a W/O emulsification with solvent evaporation technique. Microspheres were generated in high yield (80 wgt%) and vancomycin incorporation, confirmed using the displacement of DSC thermograms, had no significant effect on microsphere size distribution (5-50 microns). The vancomycin encapsulation efficiency was high (> 64%) and release profiles were characterized by a substantial initial burst release and a subsequent low-level sustained release extending up to 30 days depending upon the fabrication polymer, % vancomycin loading and incubation medium used. In both Hank's buffer and newborn calf serum the mean total cumulative release of vancomycin from microspheres increased significantly with theoretical percentage loading.
Atkins
T W
TW
Department of Pharmaceutical and Biological Sciences, Aston University, Birmingham, UK.
Peacock
S J
SJ
Yates
D J
DJ
D000818:Animals; D000900:Anti-Bacterial Agents / Q000008:administration & dosage / Q000737:chemistry* / Q000493:pharmacokinetics; D001672:Biocompatible Materials / Q000008:administration & dosage / Q000737:chemistry* / Q000493:pharmacokinetics; D002021:Buffers; D002417:Cattle; D002626:Chemistry, Pharmaceutical; D006863:Hydrogen-Ion Concentration; D019344:Lactic Acid / Q000008:administration & dosage / Q000737:chemistry* / Q000493:pharmacokinetics; D008863:Microspheres; D010316:Particle Size; D011100:Polyglycolic Acid / Q000008:administration & dosage / Q000737:chemistry* / Q000493:pharmacokinetics; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011108:Polymers / Q000008:administration & dosage / Q000737:chemistry* / Q000493:pharmacokinetics; D014640:Vancomycin / Q000008:administration & dosage / Q000737:chemistry* / Q000493:pharmacokinetics
D000900:Anti-Bacterial Agents; D001672:Biocompatible Materials; D002021:Buffers; D011108:Polymers; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011100:Polyglycolic Acid; D019344:Lactic Acid; D014640:Vancomycin