Modulation of AMPA receptor subunits GluR1 and GluR2/3 in the rat cerebellum in an experimental hepatic encephalopathy model.
The immunohistochemical expression and distribution of the AMPA-selective receptor subunits GluR1 and GluR2/3 were investigated in the rat cerebellum following portocaval anastomosis (PCA) at 1 and 6 months. With respect to controls, GluR1 and GluR2/3 immunoreactivities increased over 1 to 6 months following PCA, although immunolabelling patterns for both antibodies were different at the two analysed times. GluR1 immunoreactivity was expressed by Bergmann glial cells, which showed immunoreactive glial processes crossing the molecular layer at 6 months following PCA. The GluR2/3 subunit was expressed by Purkinje neurons and moderately expressed by neurons of the granule cell layer. Immunoreactivity for GluR2/3 was detectable in cell bodies and dendrites of Purkinje cells in young control cerebella, whereas GluR2/3 immunoreactivity was scarce 1 month post PCA. However, despite a lack of immunoreactivity in the Purkinje somata and main processes of adult control rats, GluR2/3 immunoreactivity was strongly enhanced in Purkinje neurons following long-term PCA. These findings suggest that the localization of the GluR2/3 subunit in Purkinje cells undergoes an alteration and/or reorganization as a consequence of long-term PCA. The combination of enhanced GluR immunoreactivity in long-term PCA, both in Bergmann glial cells and in Purkinje neurons, suggests some degree of neuro-glial interaction, possibly through glutamate receptors, in this type of encephalopathy.
Suárez
I
I
Dpto. Biología Celular y Genética, Universidad de Alcalá, Alcalá de Henares, Madrid, Spain. bcisn@alcala.es
Bodega
G
G
Fernández
B
B
D000818:Animals; D004195:Disease Models, Animal; D018698:Glutamic Acid / Q000633:toxicity; D006501:Hepatic Encephalopathy / Q000378:metabolism*; D007150:Immunohistochemistry; D008297:Male; D009457:Neuroglia / Q000737:chemistry; D011167:Portacaval Shunt, Surgical; D011689:Purkinje Cells / Q000737:chemistry*; D051381:Rats; D017207:Rats, Sprague-Dawley; D018091:Receptors, AMPA / Q000032:analysis* / Q000737:chemistry
D018091:Receptors, AMPA; D018698:Glutamic Acid