title: Expression of fos and jun genes in human skeletal muscle after exercise.
abstract: It is believed that the induction of the fos and jun gene family of transcription factors might be at the origin of genetic events leading to the differential regulation of muscle-specific genes. We have investigated the effect of a 30-min running bout in untrained subjects on the expression of the mRNAs of all members of the fos and jun gene families, including c-fos, fosB, fosBdel, fra-1, and fra-2 as well as c-jun, junB, and junD. While the fos family members were transiently upregulated 10- to 20-fold (an exception being fra-2) the induction of the jun family members was up to 3-fold only. The induction of c-fos could also be demonstrated at the protein level. Both c-fos and c-jun mRNAs were coinduced in muscle fiber nuclei. The induction was not restricted to a particular fiber type, as expected from established muscle fiber recruitment schemes, but followed a "patchy" pattern confined to certain regions of the muscle. The signals leading to the expression of these immediate early genes are therefore unclear.
authors.affiliation: Department of Anatomy, University of Bern, Switzerland.
authors.forename: A
authors.initials: A
authors.lastname: Puntschart
authors.forename: E
authors.initials: E
authors.lastname: Wey
authors.forename: K
authors.initials: K
authors.lastname: Jostarndt
authors.forename: M
authors.initials: M
authors.lastname: Vogt
authors.forename: M
authors.initials: M
authors.lastname: Wittwer
authors.forename: H R
authors.initials: HR
authors.lastname: Widmer
authors.forename: H
authors.initials: H
authors.lastname: Hoppeler
authors.forename: R
authors.initials: R
authors.lastname: Billeter
mesh_terms: D000328:Adult; D017931:DNA Primers; D015444:Exercise; D005260:Female; D005786:Gene Expression Regulation; D016762:Genes, fos; D016758:Genes, jun; D006801:Humans; D017403:In Situ Hybridization; D008297:Male; D005810:Multigene Family; D018482:Muscle, Skeletal / Q000166:cytology / Q000502:physiology*; D005082:Physical Exertion / Q000502:physiology*; D016133:Polymerase Chain Reaction; D016760:Proto-Oncogene Proteins c-fos / Q000096:biosynthesis*; D016755:Proto-Oncogene Proteins c-jun / Q000096:biosynthesis*; D012333:RNA, Messenger / Q000096:biosynthesis; D012420:Running; D014158:Transcription, Genetic
chemical_list: D017931:DNA Primers; D016760:Proto-Oncogene Proteins c-fos; D016755:Proto-Oncogene Proteins c-jun; D012333:RNA, Messenger