Endogenous monocyte chemoattractant protein-1 recruits monocytes in the zymosan peritonitis model.
The role of monocyte chemoattractant protein-1 (MCP-1) in the recruitment of blood-derived monocytes in a model of zymosan peritoneal inflammation was investigated. After zymosan injection (1 mg) a rapid influx of polymorphonuclear leukocytes (PMN) and monocytes into the peritoneal cavity associated with mouse MCP-1 (JE) gene activation and protein secretion in the exudates occurred. MCP-1 production (maximal at 4 h) preceded the accumulation of monocytes (F4/80-positive cells, maximally recovered between 16 and 24 h). Treatment of mice with a single injection of anti-mouse MCP-1 antibody inhibited 16-h monocyte accumulation by approximately 40%, however, a significant decrease in the number of PMN was also measured. Finally, intraperitoneal injection of murine recombinant MCP-1 (1 microg) produced a selective accumulation of monocytes (F4/80-positive cells) into the peritoneal cavity. In conclusion, we show the novel existence of a strict relationship between MCP-1 production and leukocyte accumulation in this model of acute inflammation.
Ajuebor
M N
MN
Department of Biochemical Pharmacology, The William Harvey Research Institute, London, United Kingdom.
Flower
R J
RJ
Hannon
R
R
Christie
M
M
Bowers
K
K
Verity
A
A
Perretti
M
M
D000208:Acute Disease; D000818:Animals; D018932:Chemokine CCL2 / Q000502:physiology*; D002634:Chemotaxis, Leukocyte; D003907:Dexamethasone / Q000494:pharmacology; D004195:Disease Models, Animal; D005938:Glucocorticoids / Q000494:pharmacology; D016177:Macrophage-1 Antigen / Q000502:physiology; D008297:Male; D051379:Mice; D009000:Monocytes / Q000502:physiology*; D010538:Peritonitis / Q000276:immunology* / Q000473:pathology; D014747:Vinblastine / Q000494:pharmacology; D015054:Zymosan
D018932:Chemokine CCL2; D005938:Glucocorticoids; D016177:Macrophage-1 Antigen; D014747:Vinblastine; D003907:Dexamethasone; D015054:Zymosan