title: YDL142c encodes cardiolipin synthase (Cls1p) and is non-essential for aerobic growth of Saccharomyces cerevisiae.
abstract: The unassigned open reading frame YDL142c was identified to code for cardiolipin synthase, Cls1p. A cls1 deletion strain is viable on glucose, galactose, ethanol, glycerol and lactate containing media, although the growth rate on non-fermentable carbon sources is decreased. Mitochondria of the cls1 mutant are devoid of cardiolipin but accumulate the cardiolipin precursor phosphatidylglycerol when grown on non-fermentable carbon sources. Specific activity of phosphatidylglycerolphosphate synthase in cls1 is reduced to 30-75% of the wild-type level. Amounts of mitochondrial cytochromes and activity of cytochrome c oxidase, however, are not affected in the cls1 deletion strain. Collectively, these data indicate that cardiolipin is not essential for aerobic growth of Saccharomyces cerevisiae.
authors.affiliation: Institut für Biochemie und Lebensmittelchemie, Technische Universität Graz, Austria.
authors.forename: G
authors.initials: G
authors.lastname: Tuller
authors.forename: C
authors.initials: C
authors.lastname: Hrastnik
authors.forename: G
authors.initials: G
authors.lastname: Achleitner
authors.forename: U
authors.initials: U
authors.lastname: Schiefthaler
authors.forename: F
authors.initials: F
authors.lastname: Klein
authors.forename: G
authors.initials: G
authors.lastname: Daum
mesh_terms: D000693:Anaerobiosis; D003580:Cytochromes; D003576:Electron Transport Complex IV / Q000378:metabolism; D008565:Membrane Proteins; D008928:Mitochondria / Q000737:chemistry / Q000201:enzymology; D016366:Open Reading Frames; D010743:Phospholipids / Q000032:analysis; D016133:Polymerase Chain Reaction; D012441:Saccharomyces cerevisiae / Q000201:enzymology* / Q000235:genetics / Q000254:growth & development*; D017384:Sequence Deletion; D017855:Transferases (Other Substituted Phosphate Groups) / Q000235:genetics / Q000378:metabolism*
chemical_list: D003580:Cytochromes; D008565:Membrane Proteins; D010743:Phospholipids; D003576:Electron Transport Complex IV; D017855:Transferases (Other Substituted Phosphate Groups); C100091:cardiolipin synthetase