Two chaperone sites in Hsp90 differing in substrate specificity and ATP dependence.
The abundant molecular chaperone Hsp90 is a key regulator of protein structure in the cytosol of eukaryotic cells. Although under physiological conditions a specific subset of proteins is substrate for Hsp90, under stress conditions Hsp90 seems to perform more general functions. However, the underlying mechanism of Hsp90 remained enigmatic. Here, we analyzed the function of conserved Hsp90 domains. We show that Hsp90 possesses two chaperone sites located in the N- and C-terminal fragments, respectively. The C-terminal fragment binds to partially folded proteins in an ATP-independent way potentially regulated by cochaperones. The N-terminal domain contains a peptide binding site that seems to bind preferentially peptides longer than 10 amino acids. Peptide dissociation is induced by ATP binding. Furthermore, the antitumor drug geldanamycin both inhibits the weak ATPase of Hsp90 and stimulates peptide release. We propose that the existence of two functionally different chaperone sites together with a substrate-selecting set of cochaperones allows Hsp90 to guide the folding of a subset of target proteins and, at the same time, to exhibit general chaperone functions.
Scheibel
T
T
Institut für Biophysik und Physikalische Biochemie, Universität Regensburg, 93040 Regensburg, Germany.
Weikl
T
T
Buchner
J
J
D000255:Adenosine Triphosphate / Q000378:metabolism; D001665:Binding Sites; D002950:Citrate (si)-Synthase / Q000737:chemistry*; D003001:Cloning, Molecular; D005656:Fungal Proteins / Q000737:chemistry; D018841:HSP90 Heat-Shock Proteins / Q000737:chemistry*; D007328:Insulin / Q000737:chemistry*; D018832:Molecular Chaperones / Q000737:chemistry*; D010455:Peptides / Q000737:chemistry; D011485:Protein Binding; D011489:Protein Denaturation; D017510:Protein Folding; D012441:Saccharomyces cerevisiae; D013379:Substrate Specificity
D005656:Fungal Proteins; D018841:HSP90 Heat-Shock Proteins; D007328:Insulin; D018832:Molecular Chaperones; D010455:Peptides; D000255:Adenosine Triphosphate; D002950:Citrate (si)-Synthase