title: Structural modification of fibroblast growth factor-binding heparan sulfate at a determinative stage of neural development.
abstract: Heparan sulfate (HS) glycosaminoglycans are essential modulators of fibroblast growth factor (FGF) activity and appear to act by coupling particular forms of FGF to appropriate FGF receptors. During neural development, one particular HS proteoglycan is able to rapidly switch its potentiating activity from FGF-2, as neural precursor cell proliferation occurs, to FGF-1, as neuronal differentiation occurs. Using various analytical techniques, including chemical and enzymatic cleavage, low pressure chromatography, and strong anion-exchange high performance liquid chromatography, we have analyzed the different HSs expressed during these crucial developmental stages. There are distinct alterations in patterns of 6-O-sulfation, total chain length, and the number of sulfated domains of the HS from the more mature embryonic brain. These changes correlate with a switch in the ability of the HS to potentiate the actions of FGF-1 in triggering cell differentiation. It thus appears that each HS pool is designed to function in the modulation of an intricate interaction with a specific growth factor and its cognate receptor, and suggests tightly regulated expression of specific, bioactive disaccharide sequences. The data can be used to construct a simple model of controlled variations in HS chain structure which have functional consequences at a crucial stage of neuronal maturation.
authors.affiliation: Department of Anatomy and Cell Biology, University of Melbourne, Victoria, Australia 3052.
authors.forename: Y G
authors.initials: YG
authors.lastname: Brickman
authors.forename: M D
authors.initials: MD
authors.lastname: Ford
authors.forename: J T
authors.initials: JT
authors.lastname: Gallagher
authors.forename: V
authors.initials: V
authors.lastname: Nurcombe
authors.forename: P F
authors.initials: PF
authors.lastname: Bartlett
authors.forename: J E
authors.initials: JE
authors.lastname: Turnbull
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D002850:Chromatography, Gel; D002851:Chromatography, High Pressure Liquid; D005346:Fibroblast Growth Factors / Q000378:metabolism*; D006497:Heparan Sulfate / Q000737:chemistry / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D051379:Mice; D008956:Models, Chemical; D015394:Molecular Structure; D009608:Nitrous Acid / Q000737:chemistry; D009844:Oligosaccharides / Q000737:chemistry
chemical_list: D005346:Fibroblast Growth Factors; D006497:Heparan Sulfate; D009608:Nitrous Acid; D009844:Oligosaccharides