Field inversion gel electrophoresis as a typing system for group A streptococcus.
Field inversion gel electrophoresis (FIGE) was performed on 139 isolates of group A streptococcus (GAS) representing 72 different M types. Multiple isolates were tested for 27 M types (range, 2-19). Each of the M types tested had at least one unique banding pattern that was not observed in any other M type. For 23 of 27 M types for which multiple isolates were available, band profiles of the isolates were the same or differed by < 3 bands and are considered indistinguishable or closely related. These data demonstrate that FIGE of the DNA of GAS identifies differences between and within M types.
Martin
J M
JM
Department of Pediatrics, Children's Hospital of Pittsburgh, Pennsylvania 15213, USA. martinju@chplink.chp.edu
Wald
E R
ER
Green
M
M
D015373:Bacterial Typing Techniques; D004269:DNA, Bacterial / Q000032:analysis* / Q000235:genetics; D016521:Electrophoresis, Gel, Pulsed-Field / Q000379:methods*; D012150:Polymorphism, Restriction Fragment Length; D013297:Streptococcus pyogenes / Q000145:classification* / Q000235:genetics
D004269:DNA, Bacterial