[Effect of various compounds on electrophoretic spectra of multiple molecular forms of peroxidase].
The effect of different reagents to colour intensity on PAA gel bands using benzidine was studied. All the investigated reagents were divided into three groups 1) reagents with no effect on visualization of peroxidase molecular forms colour intensity; 2) those relatively equally decreasing colour intensity; 3) selective effect on visualization of peroxidase molecular forms. Ferulic, ascorbic and indole-3-acetic acids, phloroglucin and hydroquinone are most interesting in our investigation. Use of these reagents allows increasing the differences between electrophoretic pictures during comparative physiological and genetic experiments. Possible mechanisms of the mentioned reagents influence on efficiency of peroxidase benzidine interaction are discussed.
Toptikov
V A
VA
D'iachenko
L F
LF
Totskiĭ
V N
VN
D001205:Ascorbic Acid / Q000494:pharmacology; D003373:Coumaric Acids / Q000494:pharmacology; D004591:Electrophoresis, Polyacrylamide Gel; D006873:Hydroquinones / Q000494:pharmacology; D007202:Indicators and Reagents / Q000494:pharmacology*; D007210:Indoleacetic Acids / Q000494:pharmacology; D007527:Isoenzymes / Q000187:drug effects* / Q000302:isolation & purification; D010544:Peroxidases / Q000187:drug effects* / Q000302:isolation & purification
D003373:Coumaric Acids; D006873:Hydroquinones; D007202:Indicators and Reagents; D007210:Indoleacetic Acids; D007527:Isoenzymes; C030737:indoleacetic acid; C004999:ferulic acid; D010544:Peroxidases; D001205:Ascorbic Acid; C031927:hydroquinone
Vliianie nekotorykh soedineniĭ na élektroforeticheskie spektry mnozhestvennykh molekuliarnykh form peroksidazy.