title: Molecular epidemiology of rabbit haemorrhagic disease virus outbreaks in France during 1988 to 1995.
abstract: In order to evaluate genetic variation between rabbit haemorrhagic disease virus (RHDV) isolates and to derive phylogenetic relationships, 56 virus isolates collected from various parts of France over a 7 year period (1988 to 1995) were examined. Analyses were carried out by direct nucleotide sequencing of PCR fragments of three genomic regions encoding the capsid protein (VP60) (regions A and B) and a non-structural protein (region C). Multiple sequence alignments revealed maximum nucleotide divergence of 7.6, 9.4 and 8.7% for regions A, B and C, respectively, indicating a high level of conservation between isolates. Irrespective of the genomic region analysed, phylogenetic analyses carried out using various methods allowed the identification of three genogroups; distribution of isolates within these genogroups appears to be more related to the year of their collection than to their geographical origin. The possible evolution of RHDV is discussed.
authors.affiliation: Unité de Virologie, Immunologie, Parasitologie Aviaires et Cunicoles, Centre National d'Etudes Vétérinaires et Alimentaires, Zoopôle, Ploufragan, France. Ghislaine.LEGALL.CNEVA@zoopole.asso.fr
authors.forename: G
authors.initials: G
authors.lastname: Le Gall
authors.forename: C
authors.initials: C
authors.lastname: Arnauld
authors.forename: E
authors.initials: E
authors.lastname: Boilletot
authors.forename: J P
authors.initials: JP
authors.lastname: Morisse
authors.forename: D
authors.initials: D
authors.lastname: Rasschaert
mesh_terms: D000818:Animals; D017250:Caliciviridae Infections / Q000453:epidemiology / Q000662:veterinary* / Q000821:virology; D004196:Disease Outbreaks / Q000662:veterinary*; D005602:France / Q000453:epidemiology; D017929:Hemorrhagic Disease Virus, Rabbit / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification; D010802:Phylogeny; D011817:Rabbits; D012376:Rodent Diseases / Q000453:epidemiology / Q000821:virology; D017361:Viral Nonstructural Proteins / Q000235:genetics*; D015678:Viral Structural Proteins / Q000235:genetics*
chemical_list: D017361:Viral Nonstructural Proteins; D015678:Viral Structural Proteins; C081149:viral protein 60, rabbit hemorrhagic disease virus