title: Unusual properties of thermally sensitive oligomer-enzyme conjugates of poly(N-isopropylacrylamide)-trypsin.
abstract: Reversible soluble-insoluble oligomer-enzyme conjugates have been prepared by conjugating a thermally sensitive oligomer, poly(N-isopropylacrylamide) [poly(NIPAAm)] to trypsin. The conjugates can catalyze enzymatic reactions in solution and then may be separated from the solution by thermal precipitation. One special feature of the conjugates is that every poly(NIPAAm) chain has only one end attachment to the enzyme, so that the loss of enzymatic activity due to steric hindrance should be minimized. Conjugates with various numbers of oligomer chains per trypsin molecule were prepared. Surprisingly, the conjugates increased in enzymatic activity with increasing oligomer conjugation to the native trypsin. The trypsin active sites in the conjugates were accessible to large molecules, such as soybean trypsin inhibitor (MW = 21,500). The enzyme conjugates were more stable than native trypsin, both in solution and in the precipitated phase. On the other hand, the conjugates lost enzymatic activity faster than native trypsin when the temperature was repeatedly cycled through the lower critical solution temperature (LCST) of the poly(NIPAAm). The recovery of the conjugates by thermal precipitation in each cycle was over 95% even after 14 cycles through the LCST.
authors.affiliation: Center for Bioengineering, University of Washington, Seattle 98195, USA.
authors.forename: Z
authors.initials: Z
authors.lastname: Ding
authors.forename: G
authors.initials: G
authors.lastname: Chen
authors.forename: A S
authors.initials: AS
authors.lastname: Hoffman
mesh_terms: D000178:Acrylamides / Q000737:chemistry*; D002384:Catalysis; D004355:Drug Stability; D004356:Drug Storage; D004950:Esterases / Q000378:metabolism; D006358:Hot Temperature; D007700:Kinetics; D014357:Trypsin / Q000737:chemistry*
chemical_list: D000178:Acrylamides; C067295:N-isopropylacrylamide; D004950:Esterases; D014357:Trypsin