Enzymatic mutation detection. Procedure for screening and mapping of mutations by immobilised endonuclease VII.
Endonuclease VII (endo VII) binds to non-pairing nucleotides in DNA. This served as the basis for the development of a mutation detection assay involving immobilised endo VII and heteroduplex DNAs made by hybridisation of mutant and wild type DNA. The use of microtiter plates allows screening of large numbers of samples. Localisation of mutations in positive samples can be done in the same assay in a second optional step.
Golz
S
S
Institut für Genetik der Universität zu Köln, Zülpicherstrasse 47, 50674 Köln, Germany.
Birkenkamp-Demtröder
K
K
Kemper
B
B
D004247:DNA / Q000235:genetics / Q000378:metabolism; D004706:Endodeoxyribonucleases / Q000378:metabolism; D004800:Enzymes, Immobilized; D005069:Evaluation Studies as Topic; D005821:Genetic Techniques / Q000706:statistics & numerical data; D009154:Mutation; D009692:Nucleic Acid Heteroduplexes / Q000235:genetics / Q000378:metabolism; D016133:Polymerase Chain Reaction; D012680:Sensitivity and Specificity
D004800:Enzymes, Immobilized; D009692:Nucleic Acid Heteroduplexes; D004247:DNA; D004706:Endodeoxyribonucleases; C035571:endodeoxyribonuclease VII