title: Molecular cloning and characterization of rat lin-10.
abstract: In Caenorhabditis elegans, the vulval induction is mediated by tyrosine kinase receptor/Ras signal transduction pathway composed of the lin-3, let-23, and let-60 products. In addition to these gene products, the lin-2, lin-7, and lin-10 products are also implicated in this pathway. Lin-2 encodes a MAGUK and lin-7 encodes a small protein with one PDZ domain. The lin-10 product has no homology to known proteins. Here, we have cloned a rat homologue of the lin-10 product and characterized it. Rat lin-10 is ubiquitously expressed in various rat tissues and distributed in both the cytosol and membrane fractions. In brain, however, rat lin-10 is distributed only in the membrane fraction and enriched in the synaptic plasma membrane and postsynaptic density fractions. These results suggest that rat lin-10 is involved at least in synaptic functions in brain.
authors.affiliation: Takai Biotimer Project, ERATO, Japan Science and Technology Corporation, Kobe, Japan.
authors.forename: N
authors.initials: N
authors.lastname: Ide
authors.forename: K
authors.initials: K
authors.lastname: Hirao
authors.forename: Y
authors.initials: Y
authors.lastname: Hata
authors.forename: M
authors.initials: M
authors.lastname: Takeuchi
authors.forename: M
authors.initials: M
authors.lastname: Irie
authors.forename: I
authors.initials: I
authors.lastname: Yao
authors.forename: M
authors.initials: M
authors.lastname: Deguchi
authors.forename: A
authors.initials: A
authors.lastname: Toyoda
authors.forename: H
authors.initials: H
authors.lastname: Nishioka
authors.forename: A
authors.initials: A
authors.lastname: Mizoguchi
authors.forename: Y
authors.initials: Y
authors.lastname: Takai
mesh_terms: D000818:Animals; D017173:Caenorhabditis elegans / Q000737:chemistry; D029742:Caenorhabditis elegans Proteins; D002478:Cells, Cultured; D003001:Cloning, Molecular; D018507:Gene Expression Regulation, Developmental / Q000235:genetics; D015801:Helminth Proteins / Q000032:analysis / Q000737:chemistry*; D006624:Hippocampus / Q000737:chemistry; D007150:Immunohistochemistry; D008565:Membrane Proteins; D009419:Nerve Tissue Proteins / Q000032:analysis / Q000737:chemistry; D011506:Proteins; D012333:RNA, Messenger / Q000032:analysis; D051381:Rats; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D013569:Synapses / Q000502:physiology
chemical_list: D029742:Caenorhabditis elegans Proteins; D015801:Helminth Proteins; D008565:Membrane Proteins; D009419:Nerve Tissue Proteins; D011506:Proteins; D012333:RNA, Messenger; C064105:lin-10 protein, C elegans