Analysis of the retinoic acid receptor alpha gene as a candidate for the pulmonary adenoma resistance 1 gene.
The retinoic acid receptor alpha (Rara) gene, which maps in the same region as the pulmonary adenoma resistance (Par1) locus on mouse chromosome 11 (Manenti G et al., Nature Genet 12:455-457, 1996), was tested as a candidate gene for Par1. We report here the analysis of loss of heterozygosity, nucleotide sequence comparison, gene expression, and biochemical activity of the Rara gene from the Mus spretus(Par1/+) and A/J (Par1/-) mouse strains. The two Rara alleles were distinguished by two amino-acid variations but had similar biochemical activity and expression levels, leading to the exclusion of Rara as a candidate Par1 gene.
Gariboldi
M
M
Division of Experimental Oncology A, Istituto Nazionale Tumori, Milan, Italy.
Vivat
V
V
De Gregorio
L
L
Manenti
G
G
Chiariello
E
E
Falvella
F S
FS
Pierotti
M A
MA
Dragani
T A
TA
D000236:Adenoma / Q000235:genetics*; D000483:Alleles; D000818:Animals; D014408:Biomarkers, Tumor / Q000235:genetics*; D015972:Gene Expression Regulation, Neoplastic; D016147:Genes, Tumor Suppressor / Q000235:genetics*; D019656:Loss of Heterozygosity; D008175:Lung Neoplasms / Q000235:genetics*; D051379:Mice; D008969:Molecular Sequence Data; D018168:Receptors, Retinoic Acid / Q000235:genetics*; D000072482:Retinoic Acid Receptor alpha
D014408:Biomarkers, Tumor; C000607072:Rara protein, mouse; D018168:Receptors, Retinoic Acid; D000072482:Retinoic Acid Receptor alpha