title: A high-resolution genotyping method for HLA-C alleles and possible shared HLA-C-B haplotypes between Japanese and Caucasians.
abstract: A genotyping system for HLA-Cw alleles using methods of PCR-single-strand conformation polymorphism (SSCP) and PCR-sequence-specific primers (SSP) was developed. Genomic DNA from 167 random healthy Japanese individuals were investigated to determine HLA-C allele frequencies and their associations with HLA-A and -B loci. Nine alleles were frequently detected: Cw*0102 (18.3%), Cw*0304 (12.0%), Cw*1202 (11.4%), Cw*0303 (10.5%), Cw*1403 (10.5%), Cw*0801 (10.2%), Cw*0702 (9.0%), Cw*1402 (6.0%) and Cw*0401 (5.1%). Several HLA-C-B haplotypes such as Cw*0303-B62, Cw*0304-B60, Cw*0501-B44, Cw*0602-B13, Cw*0602-B37, Cw*0702-B7, Cw*1202-B52, Cw*1402-B51 and Cw*1502-B51 were found to be shared by Japanese and Caucasians. With the PCR-SSCP method we could detect differences in each exon of HLA-C alleles, at a low cost and simply. This method is suitable for sequence-level matching with a relatively small number of samples.
authors.affiliation: Department of Research, Japanese Red Cross Central Blood Center, Tokyo, Japan. huiru-w@smtpgate.bcsew.edu
authors.forename: H
authors.initials: H
authors.lastname: Wang
authors.forename: K
authors.initials: K
authors.lastname: Tokunaga
authors.forename: Y
authors.initials: Y
authors.lastname: Ishikawa
authors.forename: H
authors.initials: H
authors.lastname: Tanaka
authors.forename: K
authors.initials: K
authors.lastname: Kashiwase
authors.forename: Y
authors.initials: Y
authors.lastname: Shibata
authors.forename: T
authors.initials: T
authors.lastname: Juji
mesh_terms: D000483:Alleles; D044466:Asian People; D002874:Chromosome Mapping; D005787:Gene Frequency; D005805:Genes, MHC Class I; D005838:Genotype; D015235:HLA-B Antigens / Q000235:genetics*; D015236:HLA-C Antigens / Q000235:genetics*; D006239:Haplotypes; D006801:Humans; D007564:Japan / Q000208:ethnology; D016133:Polymerase Chain Reaction; D018807:Polymorphism, Single-Stranded Conformational; D044465:White People
chemical_list: D015235:HLA-B Antigens; D015236:HLA-C Antigens