Analysis of a mosquito acetylcholinesterase gene promoter.
Insect acetylcholinesterase is the target site for organophosphorus and carbamate insecticides and point mutations in the Ace gene are associated with resistance in Drosophila melanogaster and Musca domestica. However, little is known of the genetic regulation of insect Ace genes. Here we report the isolation of four different cDNAs from an Aedes Ace locus and identification of the gene promoter. Northern analysis reveals two large (>10 kb) transcripts and one smaller transcript of 4 kb. The region containing the initiation of transcription was localized by sequencing the two 5' most cDNAs and by 5' RACE. The transcription start point was subsequently identified by primer extension and is flanked by a perfect arthropod initiator consensus sequence. The promoter lacks a TATA box but contains several matches to other consensus sequences for eukaryotic transcription factors. In common with the Drosophila Ace gene, there are also multiple potential initiators of translation (ATGs) upstream of the main open reading frame. The structure of the 5' leader and promoter is compared to that found in other insect and vertebrate Ace genes and the possibility that this locus is homologous to one of two Ace loci described in another mosquito, Culex pipiens, is discussed.
Liu
H T
HT
Department of Entomology and Center for Neuroscience, University of Wisconsin-Madison 53706, USA.
Stilwell
G
G
Anthony
N
N
Rocheleau
T
T
ffrench-Constant
R H
RH
D000110:Acetylcholinesterase / Q000235:genetics*; D000330:Aedes / Q000201:enzymology* / Q000235:genetics; D000818:Animals; D001483:Base Sequence; D001665:Binding Sites; D002874:Chromosome Mapping; D003465:Culex / Q000235:genetics; D018076:DNA, Complementary; D017344:Genes, Insect; D008969:Molecular Sequence Data; D010442:Peptide Chain Initiation, Translational; D011401:Promoter Regions, Genetic; D012045:Regulatory Sequences, Nucleic Acid; D014158:Transcription, Genetic
D018076:DNA, Complementary; D000110:Acetylcholinesterase