Prediction of the coding sequences of unidentified human genes. VIII. 78 new cDNA clones from brain which code for large proteins in vitro.
As a part of our project for accumulating sequence information of the coding regions of unidentified human genes, we herein report the sequence features of 78 new cDNA clones isolated from human brain cDNA libraries as those which may code for large proteins. The sequence data showed that the average size of the cDNA inserts and their open reading frames was 6.0 kb and 2.8 kb (925 amino acid residues), respectively, and these clones produced the corresponding sizes of protein products in an in vitro transcription/translation system. Homology search against the public databases indicated that the predicted coding sequences of 68 genes contained sequences similar to known genes, 69% of which (47 genes) were related to cell signaling/communication, nucleic acid management, and cell structure/motility. The expression profiles of these genes in 14 different tissues have been analyzed by the reverse transcription-coupled polymerase chain reaction method, and 8 genes were found to be predominantly expressed in the brain.
Ishikawa
K
K
Kazusa DNA Research Institute, Chiba, Japan.
Nagase
T
T
Nakajima
D
D
Seki
N
N
Ohira
M
M
Miyajima
N
N
Tanaka
A
A
Kotani
H
H
Nomura
N
N
Ohara
O
O
D001923:Brain Chemistry / Q000235:genetics*; D002874:Chromosome Mapping; D018076:DNA, Complementary / Q000032:analysis* / Q000737:chemistry / Q000302:isolation & purification; D015870:Gene Expression; D006801:Humans; D016366:Open Reading Frames; D016133:Polymerase Chain Reaction; D011506:Proteins / Q000145:classification / Q000235:genetics* / Q000502:physiology; D017422:Sequence Analysis, DNA / Q000379:methods; D012689:Sequence Homology, Nucleic Acid; D014018:Tissue Distribution / Q000235:genetics; D014158:Transcription, Genetic; D016335:Zinc Fingers / Q000235:genetics
D018076:DNA, Complementary; D011506:Proteins