Annexin V labels apoptotic neurons following hypoxia-ischemia.
The translocation of phosphatidylserine from the cytosol to the external surface of the plasma membrane has been documented as a characteristic feature of apoptosis in a number of cell types. Annexin V is a calcium-dependent phospholipid binding protein that has high affinity for phosphatidylserine. To investigate whether Annexin V provides a marker of apoptosis in the central nervous system we carried out histochemical analysis of its binding in the 21-day-old rat brain at various time-points following a moderate unilateral hypoxic-ischemic (HI) insult. The CA1 pyramidal neurons, which are selectively vulnerable to HI injury and that die by an apoptotic mechanism showed an increase in Annexin V binding 48-168 hours post-insult.
Walton
M
M
Department of Pharmacology, Faculty of Medicine and Health Science, University of Auckland, New Zealand.
Sirimanne
E
E
Reutelingsperger
C
C
Williams
C
C
Gluckman
P
P
Dragunow
M
M
D000818:Animals; D017304:Annexin A5 / Q000032:analysis*; D017209:Apoptosis / Q000502:physiology*; D015415:Biomarkers / Q000737:chemistry; D002545:Brain Ischemia / Q000378:metabolism* / Q000473:pathology; D053938:DNA Fragmentation; D006651:Histocytochemistry; D002534:Hypoxia, Brain / Q000378:metabolism* / Q000473:pathology; D009419:Nerve Tissue Proteins / Q000032:analysis*; D017966:Pyramidal Cells / Q000737:chemistry*; D051381:Rats; D017208:Rats, Wistar
D017304:Annexin A5; D015415:Biomarkers; D009419:Nerve Tissue Proteins