Effect of pentylenetetrazol treatment on cholecystokinin mRNA and peptide levels in rat hippocampus and cortex.
After treatment with pentylenetetrazol (PTZ), cholecystokinin (CCK) mRNA and CCK-like immunoreactivity (CCK-LI) levels were determined in rat hippocampus and cortex at different time points. In the temporal cortex treatment with 60 mg/kg PTZ, i.p., induced increases of CCK mRNA and CCK-LI levels at 2 days after the injection. In the hippocampus, a similar increase of CCK mRNA level was observed on the second day. By contrast, in the frontal cortex, CCK-LI level was increased at 10 days after the treatment with PTZ. These data show that PTZ increases both CCK mRNA and CCK-LI levels in these rat brain regions at different time.
Takeda
K
K
Research and Development Department, Dainabot, Chiba, Japan.
Nakata
K
K
Takahashi
S
S
Chikuma
T
T
Kato
T
T
D000818:Animals; D015152:Blotting, Northern; D002540:Cerebral Cortex / Q000187:drug effects* / Q000378:metabolism; D002766:Cholecystokinin / Q000235:genetics*; D003292:Convulsants; D006624:Hippocampus / Q000187:drug effects* / Q000378:metabolism; D007150:Immunohistochemistry; D008297:Male; D009479:Neuropeptides / Q000378:metabolism*; D010433:Pentylenetetrazole; D012333:RNA, Messenger / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar
D003292:Convulsants; D009479:Neuropeptides; D012333:RNA, Messenger; D002766:Cholecystokinin; D010433:Pentylenetetrazole