A physical map of the Leishmania major Friedlin genome.
An extensive physical map of the Leishmania major Friedlin genome has been assembled by the combination of fingerprint analysis of a shuttle vector cosmid library and probe hybridization. The integrated data obtained for 9004 fingerprinted clones and 974 probes have placed 91.2% of the 33.58-Mb genome into contigs representing each of the 36 chromosomes. This first-generation map has already provided a suitable framework for both high-throughput DNA sequencing and functional studies of the L. major parasite.
Ivens
A C
AC
Department of Biochemistry, Imperial College of Science, Technology and Medicine, London SW7 2AZ, UK. a.ivens@ic.ac.uk
Lewis
S M
SM
Bagherzadeh
A
A
Zhang
L
L
Chan
H M
HM
Smith
D F
DF
D000818:Animals; D016172:DNA Fingerprinting; D015342:DNA Probes; D016054:DNA, Protozoan / Q000032:analysis; D016521:Electrophoresis, Gel, Pulsed-Field; D005819:Genetic Markers; D018503:Genome, Protozoan; D018320:Leishmania major / Q000235:genetics*; D009693:Nucleic Acid Hybridization; D015183:Restriction Mapping / Q000379:methods*; D017422:Sequence Analysis, DNA
D015342:DNA Probes; D016054:DNA, Protozoan; D005819:Genetic Markers