Seroreactive species-specific lipooligosaccharides of Mycobacterium mucogenicum sp. nov. (formerly Mycobacterium chelonae-like organisms): identification and chemical characterization.
Strains of the new species Mycobacterium mucogenicum exhibit physiological and biochemical features very similar to those of the other species of the Mycobacterium fortuitum complex. To define taxonomic criteria for easy identification of M. mucogenicum, the glycolipid patterns of the reference strains and of 32 environmental and clinical isolates were examined by TLC. It was concluded that all M. mucogenicum strains of smooth colony morphology contained species-specific alkali-labile glycoconjugates. Three different patterns were observed among the strains of the smooth colony type. Fractionation followed by conventional chemical analyses of the purified glycolipids showed the specific glycolipids to be lipooligosaccharides (LOS). The three LOS showed a similar fatty acid composition consisting of straight chain (dodeca-, tetradeca-, hexadecanoyl and hexadecenoyl) and methylbranched (2,4-dimethyleicosanoyl and 2,4-dimethyleicosenoyl) fatty acyl substituents. The most commonly encountered LOS (present in 76% of the smooth strains) contained a tetraacylated pentasaccharide composed of four moles of glucose and one mole of a 2,4-di-O-methylhexose. A LOS composed of arabinose, glucose and mannose was present in 20% of the smooth strains, whereas the newly proposed type strain of M. mucogenicum (ATCC 49650) was the only strain that contained a LOS composed of glucose and galactose. Serological studies clearly differentiated most of the strains of M. mucogenicum from those of the other members of the M. fortuitum complex, and demonstrated the existence of serovars within the former species. Altogether, these data confirm the validity of the new species but show ATCC 49651 to be the most representative strain.
Muñoz
Manuel
M
Institut de Pharmacologie et de Biologie Structurale du CNRS, 205 route de Narbonne, 31077 Toulouse cedex, France.|Departamento de Genética y de Microbiología, Unidad de Microbiología, Facultad de Ciencias, Universidad Autónoma de Barcelona, 08193 Bellaterra (Barcelona), Spain.
Raynaud
Catherine
C
Institut de Pharmacologie et de Biologie Structurale du CNRS, 205 route de Narbonne, 31077 Toulouse cedex, France.
Lanéelle
Marie-Antoinette
MA
Institut de Pharmacologie et de Biologie Structurale du CNRS, 205 route de Narbonne, 31077 Toulouse cedex, France.
Julián
Esther
E
Departamento de Genética y de Microbiología, Unidad de Microbiología, Facultad de Ciencias, Universidad Autónoma de Barcelona, 08193 Bellaterra (Barcelona), Spain.
Marín
Luz María López
LML
Institut de Pharmacologie et de Biologie Structurale du CNRS, 205 route de Narbonne, 31077 Toulouse cedex, France.
Silve
Gaby
G
Institut de Pharmacologie et de Biologie Structurale du CNRS, 205 route de Narbonne, 31077 Toulouse cedex, France.
Ausina
Vicente
V
Servicio de Microbiología, Hospital Universitario Germans Trias i Pujol, 08916 Badalona, (Barcelona), Spain.
Daffé
Mamadou
M
Institut de Pharmacologie et de Biologie Structurale du CNRS, 205 route de Narbonne, 31077 Toulouse cedex, France.
Luquin
Marina
M
Departamento de Genética y de Microbiología, Unidad de Microbiología, Facultad de Ciencias, Universidad Autónoma de Barcelona, 08193 Bellaterra (Barcelona), Spain.
D000818:Animals; D006017:Glycolipids / Q000032:analysis* / Q000737:chemistry; D006801:Humans; D009161:Mycobacterium / Q000145:classification* / Q000166:cytology / Q000502:physiology; D009164:Mycobacterium Infections / Q000382:microbiology; D016926:Mycobacterium chelonae / Q000145:classification / Q000166:cytology / Q000472:pathogenicity; D011817:Rabbits; D012703:Serotyping
D006017:Glycolipids