title: Structure and expression of a novel member, FGF-16, on the fibroblast growth factor family.
abstract: We have isolated cDNA encoding a novel member (207 amino acids) of the FGF family from the rat heart by homology-based polymerase chain reaction. As this protein is the 16th documented member of the FGF family, we tentatively term it FGF-16. Among FGF family members, FGF-16 is most similar (73% amino acid identity) to FGF-9. We have also determined the structure of human FGF-16 with high amino acid sequence identity (98.6%) to rat FGF-16. Although the predicted FGF-16 amino acid sequence lacks a typical signal sequence, recombinant rat FGF-16 was efficiently secreted by Sf9 insect cells infected with recombinant baculovirus containing the cDNA. FGF-16 mRNA was predominantly expressed in the rat heart among the adult major tissues examined. The expression profile of FGF-16 mRNA was quite different from those of other members of the FGF family. In rat embryos, FGF-16 mRNA was predominantly expressed in the brown adipose tissue. However, the expression decreased greatly after birth. These results indicate that FGF-16 in embryos might play a role in development of the brown adipose tissue.
authors.affiliation: Department of Genetic Biochemistry, Kyoto University Graduate School of Pharmaceutical Sciences, Japan.
authors.forename: A
authors.initials: A
authors.lastname: Miyake
authors.forename: M
authors.initials: M
authors.lastname: Konishi
authors.forename: F H
authors.initials: FH
authors.lastname: Martin
authors.forename: N A
authors.initials: NA
authors.lastname: Hernday
authors.forename: K
authors.initials: K
authors.lastname: Ozaki
authors.forename: S
authors.initials: S
authors.lastname: Yamamoto
authors.forename: T
authors.initials: T
authors.lastname: Mikami
authors.forename: T
authors.initials: T
authors.lastname: Arakawa
authors.forename: N
authors.initials: N
authors.lastname: Itoh
mesh_terms: D002001:Adipose Tissue, Brown / Q000196:embryology / Q000254:growth & development / Q000378:metabolism; D000595:Amino Acid Sequence; D000818:Animals; D016367:Baculoviridae / Q000235:genetics; D002460:Cell Line; D018076:DNA, Complementary / Q000235:genetics / Q000302:isolation & purification; D019143:Evolution, Molecular; D051525:Fibroblast Growth Factor 9; D005346:Fibroblast Growth Factors / Q000737:chemistry* / Q000235:genetics* / Q000502:physiology; D018507:Gene Expression Regulation, Developmental; D006133:Growth Substances / Q000235:genetics; D006801:Humans; D008969:Molecular Sequence Data; D009206:Myocardium / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D051381:Rats; D011994:Recombinant Proteins / Q000737:chemistry / Q000235:genetics; D017386:Sequence Homology, Amino Acid; D018411:Spodoptera; D014018:Tissue Distribution
chemical_list: D018076:DNA, Complementary; C512705:FGF16 protein, human; C496459:FGF9 protein, human; C110776:Fgf16 protein, rat; C496461:Fgf9 protein, rat; D051525:Fibroblast Growth Factor 9; D006133:Growth Substances; D012333:RNA, Messenger; D011994:Recombinant Proteins; D005346:Fibroblast Growth Factors