The Androctonus australis garzoni scorpion venom contains toxins that selectively affect voltage-dependent K(+)-channels in cerebellum granular cells.
A purified peptide from Androctonus australis Garzoni venom (AaG) affects selectively a K(+)-current recorded from cerebellum granular cells. This current is characterized by fast activating and inactivating kinetics similar to an IA-type current. Addition of 2 microM peptide Aa1 (from Androctonus australis, toxin 1) to the external side of the channel suppressed completely and in a selective manner the IA-type current, with an IC50 value of 130 nM, whereas in the same conditions, the other potassium current, identified as delayed rectifier (Id), was not affected. Additionally, we show that another partially purified peptide (III-12) from the same venom was able to block reversibly both K(+)-currents.
Pisciotta
M
M
Coronas
F I
FI
Possani
L D
LD
Prestipino
G
G
D000818:Animals; D002478:Cells, Cultured; D002531:Cerebellum / Q000187:drug effects* / Q000502:physiology; D003594:Cytoplasmic Granules / Q000187:drug effects / Q000502:physiology; D004594:Electrophysiology; D011188:Potassium / Q000502:physiology; D015221:Potassium Channels / Q000187:drug effects* / Q000502:physiology; D051381:Rats; D017208:Rats, Wistar; D012604:Scorpion Venoms / Q000737:chemistry* / Q000633:toxicity*; D012605:Scorpions; D014118:Toxins, Biological / Q000302:isolation & purification* / Q000633:toxicity*
D015221:Potassium Channels; D012604:Scorpion Venoms; D014118:Toxins, Biological; D011188:Potassium