Mutation analysis of interleukin-5 in an asthmatic cohort.
Interleukin-5 (IL-5) is a potential candidate gene in the pathogenesis of asthma, as it is the main cytokine controlling eosinophil activity and eosinophils are pivotal in the development of airway inflammation. Mutation detection studies were performed on the IL-5 gene and the alpha-chain of its receptor in 30 asthmatic and 30 nonasthmatic subjects. Single-strand conformational polymorphism (SSCP) and heteroduplex analysis (HA) did not reveal any change from the reported normal sequence in all 4 exons of IL-5 as well as the promoter and 3'-untranslated regions of the gene. No SSCP variations were seen within the complete coding sequence of the IL-5 receptor alpha-chain. Mutations of the IL-5 gene coding region, its promoter and receptor are unlikely to be common causes of an inherited predisposition to asthma.
Pereira
E
E
Department of Genetics, University of Western Australia, Perth.
Goldblatt
J
J
Rye
P
P
Sanderson
C
C
Le Souëf
P
P
D001249:Asthma / Q000235:genetics*; D004252:DNA Mutational Analysis; D005091:Exons / Q000235:genetics; D006801:Humans; D006969:Hypersensitivity, Immediate / Q000235:genetics; D015848:Interleukin-5 / Q000235:genetics* / Q000378:metabolism; D008137:Longitudinal Studies; D009154:Mutation; D009692:Nucleic Acid Heteroduplexes / Q000235:genetics; D018807:Polymorphism, Single-Stranded Conformational; D011401:Promoter Regions, Genetic; D018123:Receptors, Interleukin / Q000235:genetics; D053649:Receptors, Interleukin-5
D015848:Interleukin-5; D009692:Nucleic Acid Heteroduplexes; D018123:Receptors, Interleukin; D053649:Receptors, Interleukin-5