title: Coculture with follicular shell pieces can enhance the developmental competence of pig oocytes after in vitro fertilization: relevance to intracellular glutathione.
abstract: The present study examined the effect of follicular shell pieces (FSP) during in vitro maturation (IVM) of porcine oocytes on 1) in vitro fertilization (IVF) parameters, 2) subsequent embryo development, 3) oocyte glutathione (GSH) concentration, and 4) viability after embryo transfer. Cumulus-oocyte complexes were cultured in North Carolina State University (NCSU) 23 medium containing porcine follicular fluid, cysteine, and hormonal supplements and with or without FSP for 20-22 h. They were then cultured in the same medium but without hormonal supplements for an additional 20-22 h. After culture, cumulus-free oocytes were coincubated with frozen-thawed spermatozoa for 5-6 h. Putative zygotes were transferred to NCSU 23 containing 0.4% BSA and cultured for 144 h. In comparisons between the presence and absence of FSP, no differences were observed in fertilization parameters. At 48 h, no mean differences were found in cleavage rates. However, at 144 h, the proportion of embryos that developed to the blastocyst stage was significantly (p < 0.01) higher (18% vs. 36%) for oocytes cocultured with FSP. A significantly (p < 0.05) higher GSH concentration was found in oocytes matured with FSP as determined by dithionitrobenzoic acid-glutathione disulfide (DTNB-GSSG) reductase recycling assay. Transfer of embryos to 9 recipients resulted in 5 pregnancies with the birth of 18 live piglets. The results provide clear evidence of the beneficial effect of FSP during IVM of pig oocytes cultured in the presence of cysteine on subsequent embryo development to the blastocyst stage. The birth of piglets confirms the viability of IVM-IVF-derived embryos.
authors.affiliation: Department of Animal Sciences, University of Missouri-Columbia, 65211, USA.
authors.forename: L R
authors.initials: LR
authors.lastname: Abeydeera
authors.forename: W H
authors.initials: WH
authors.lastname: Wang
authors.forename: T C
authors.initials: TC
authors.lastname: Cantley
authors.forename: A
authors.initials: A
authors.lastname: Rieke
authors.forename: B N
authors.initials: BN
authors.lastname: Day
mesh_terms: D000818:Animals; D018920:Coculture Techniques; D004624:Embryo Transfer; D005260:Female; D005307:Fertilization in Vitro; D005978:Glutathione / Q000378:metabolism*; D008297:Male; D009865:Oocytes / Q000254:growth & development*; D006080:Ovarian Follicle / Q000502:physiology*; D011247:Pregnancy; D011256:Pregnancy Outcome; D013552:Swine
chemical_list: D005978:Glutathione