title: Systematic gain-of-function genetics in Drosophila.
abstract: A modular misexpression system was used to carry out systematic gain-of-function genetic screens in Drosophila. The system is based on inducible expression of genes tagged by insertion of a P-element vector carrying a GAL4-regulated promoter oriented to transcribe flanking genomic sequences. To identify genes involved in eye and wing development, the 2300 independent lines were screened for dominant phenotypes. Among many novel genes, the screen identified known genes, including hedgehog and decapentaplegic, implicated in these processes. A genetic interaction screen for suppressors of a cell migration defect in a hypomorphic slow border cells mutant identified known genes with likely roles in tyrosine kinase signaling and control of actin cytoskeleton, among many novel genes. These studies demonstrate the ability of the modular misexpression system to identify developmentally important genes and suggest that it will be generally useful for genetic interaction screens.
authors.affiliation: Department of Embryology, Carnegie Institution of Washington, 115 West University Parkway, Baltimore, MD 21210, USA. rorth@mail1.ciwemb.edu
authors.forename: P
authors.initials: P
authors.lastname: Rørth
authors.forename: K
authors.initials: K
authors.lastname: Szabo
authors.forename: A
authors.initials: A
authors.lastname: Bailey
authors.forename: T
authors.initials: T
authors.lastname: Laverty
authors.forename: J
authors.initials: J
authors.lastname: Rehm
authors.forename: G M
authors.initials: GM
authors.lastname: Rubin
authors.forename: K
authors.initials: K
authors.lastname: Weigmann
authors.forename: M
authors.initials: M
authors.lastname: Milán
authors.forename: V
authors.initials: V
authors.lastname: Benes
authors.forename: W
authors.initials: W
authors.lastname: Ansorge
authors.forename: S M
authors.initials: SM
authors.lastname: Cohen
mesh_terms: D000818:Animals; D022762:CCAAT-Enhancer-Binding Proteins; D002465:Cell Movement / Q000235:genetics; D004268:DNA-Binding Proteins; D004330:Drosophila / Q000235:genetics* / Q000254:growth & development; D029721:Drosophila Proteins; D005123:Eye / Q000254:growth & development; D005260:Female; D018507:Gene Expression Regulation, Developmental; D005799:Genes, Dominant; D017344:Genes, Insect; D016678:Genome; D008297:Male; D009154:Mutation; D010641:Phenotype; D029701:Saccharomyces cerevisiae Proteins; D013489:Suppression, Genetic; D014157:Transcription Factors / Q000235:genetics; D014921:Wings, Animal / Q000254:growth & development
chemical_list: D022762:CCAAT-Enhancer-Binding Proteins; D004268:DNA-Binding Proteins; D029721:Drosophila Proteins; C052130:GAL4 protein, S cerevisiae; D029701:Saccharomyces cerevisiae Proteins; D014157:Transcription Factors; C077246:slbo protein, Drosophila