Role of nuclear architecture in the initiation of eukaryotic DNA replication.
The eukaryotic genome is compacted in the cell nucleus, in a way that allows its faithful and ordered replication each cell cycle. Chromatin is organized into topologically constrained loops that are anchored to the nuclear matrix by specific attachment regions (SARs). Chromatin loops were proposed to correspond to replication units. In particular, it has been suggested that replication origins coincide with SARs. Critical examination of these hypotheses has long been hampered by the elusive nature of higher eukaryotic DNA replication origins and termini. In recent years, however, a number of loci have been mapped for both SARs and replication units, and studies on the nuclear localization of replicating DNA and replication proteins have begun. We review these data and argue that they question this model. We then try to delineate other aspects of chromosome compartmentalization and cell-cycle remodeling which might be responsible for the specification and activation of metazoan DNA replication origins.
Hyrien
O
O
Laboratoire de Génétique Moléculaire, Ecole Normale Supérieure, Paris, France.
Maric
C
C
Lucas
I
I
D000818:Animals; D004261:DNA Replication / Q000502:physiology*; D005057:Eukaryotic Cells / Q000378:metabolism / Q000502:physiology*; D006801:Humans; D008954:Models, Biological; D008957:Models, Genetic; D015530:Nuclear Matrix / Q000235:genetics / Q000502:physiology*