title: Organization and transcriptional characterization of the cat1 gene cluster in Acinetobacter lwoffi K24.
abstract: Previously, we have reported that two clustered cat genes from Acenitobacter lwoffi K24 had different arrangements, catB1C1A1 and catB2A2C2 (Kim, S.I., S.-H. Leem, J.-S. Choi, Y.H. Chung, S. Kim, Y.-M. Park, Y.K. Park, Y.N. Lee, and K.-S. Ha. 1997, J. Bacteriol. 179, 5226-5231). By further analysis of the organization of the cat1 gene cluster, we obtained a complete sequence of the catB1 gene, which encoded 40.8-kDa polypeptide containing 379 amino acids, and found a open reading frame (ORF) coding a putative regulatory protein in upstream region of catB1 on plasmid pCD1-1. This ORF encoded 34.2-kDa polypeptide containing 379 amino acids and had more than 40% identity with catR, LysR family regulatory protein of Pseudomonas putida. RT-PCR, Northern blot analysis and primer extension assay for transcriptional analysis of the cat1 gene cluster revealed that the catB1C1 genes were cotranscribed and the catA1 gene was independently transcribed.
authors.affiliation: Biomolecule Research Group, Korea Basic Science Institute, Taejon, Korea. ksi@comp.kbsi.re.kr
authors.forename: S I
authors.initials: SI
authors.lastname: Kim
authors.forename: S H
authors.initials: SH
authors.lastname: Leem
authors.forename: J S
authors.initials: JS
authors.lastname: Choi
authors.forename: K S
authors.initials: KS
authors.lastname: Ha
mesh_terms: D000150:Acinetobacter / Q000235:genetics* / Q000378:metabolism; D000595:Amino Acid Sequence; D001483:Base Sequence; D002396:Catechols / Q000378:metabolism; D003001:Cloning, Molecular; D017931:DNA Primers / Q000235:genetics; D004269:DNA, Bacterial / Q000235:genetics; D005798:Genes, Bacterial; D008969:Molecular Sequence Data; D005810:Multigene Family; D016366:Open Reading Frames; D011401:Promoter Regions, Genetic; D016958:Pseudomonas putida / Q000235:genetics; D015183:Restriction Mapping; D017386:Sequence Homology, Amino Acid; D012689:Sequence Homology, Nucleic Acid; D014158:Transcription, Genetic
chemical_list: D002396:Catechols; D017931:DNA Primers; D004269:DNA, Bacterial; C034221:catechol