title: Mutants of the phytopathogenic fungus magnaporthe grisea deficient in alternative, cyanide-resistant, respiration
abstract: The phytopathogenic fungus Magnaporthe grisea has a cyanide-resistant respiratory pathway. The fungicide SSF-126 ((E)-2-methoxyimino-N-methyl-2-(2-phenoxyphenyl) acetamide) blocks the cytochrome electron transport of M. grisea and induces the alternative respiratory pathway. Twelve mutants of M. grisea more susceptible to SSF-126 than wild type were identified after N-methyl-N'-nitro-N-nitrosoguanidine mutagenesis. Five mutants retained a reduced alternative respiration activity, and seven mutants lacked alternative pathway activity. A monoclonal antibody against the maize alternative oxidase cross-reacted against a 40-kDa mitochondrial protein of M. grisea, indicating that the 40-kDa protein is an alternative oxidase. Immunoblot analysis indicated that the seven completely deficient mutants grouped into two classes: four mutants produced the 40-kDa proteins while the other three mutants failed to produce the functional protein. Copyright 1997 Academic Press. Copyright 1997 Academic Press
authors.affiliation: Aburahi Laboratories, Shionogi & Co., Ltd., 1405 Gotanda, Koka, Shiga, 520-34, Japan.
authors.initials: H
authors.lastname: Yukioka
authors.initials: R
authors.lastname: Tanaka
authors.initials: S
authors.lastname: Inagaki
authors.initials: K
authors.lastname: Katoh
authors.initials: N
authors.lastname: Miki
authors.initials: A
authors.lastname: Mizutani
authors.initials: M
authors.lastname: Masuko
authors.initials: H
authors.lastname: Kunoh