Separation of alditols of interest in food products by high-performance anion-exchange chromatography with pulsed amperometric detection.
High-performance anion-exchange chromatography (HPAEC)-pulsed amperometric detection (PAD) employing a CarboPac MA 1 column was investigated with respect to mobile phase composition, linear response characteristics, repeatability, reproducibility and sensitivity with different alditols used as sugar substitutes in food and confectionery products. The energy-reduced bulk sweeteners isomalt and maltitol were well resolved in less than 25 min by isocratic elution with 600 mM sodium hydroxide solution. HPAEC-PAD was also successfully applied to the determination of alditols in sugar-free products and a low-calorie sweetener containing sorbitol, mannitol and fructose at different levels.
Corradini
C
C
Istituto di Cromatografia del C.N.R., Rome, Italy.
Canali
G
G
Cogliandro
E
E
Nicoletti
I
I
D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004563:Electrochemistry; D005632:Fructose / Q000302:isolation & purification; D008353:Mannitol / Q000302:isolation & purification; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013012:Sorbitol / Q000302:isolation & purification; D013402:Sugar Alcohols / Q000302:isolation & purification*; D013549:Sweetening Agents / Q000737:chemistry*; D013997:Time Factors
D013402:Sugar Alcohols; D013549:Sweetening Agents; D005632:Fructose; D008353:Mannitol; D013012:Sorbitol