title: Rat peritoneal macrophages express endothelin ET(B) but not endothelin ET(A) receptors.
abstract: The properties of endothelin receptors on rat peritoneal macrophages were examined in in vitro receptor autoradiographic binding experiments and in a reverse transcription-polymerase chain reaction (RT-PCR) study. Dense and specific [(125)I]endothelin-1 binding sites were detected on the macrophages. [(125)I]Tyr13-Suc-[Glu9,Ala(11,15)]-endothelin-1(8-21) , IRL1620, a selective endothelin ET(B) receptor ligand, but not [(125)I](N-[(hexahydro-1-azepinyl)carbonyl])L-Leu(1-Me)D-Trp-D-Tyr , PD151242, a selective endothelin ET(A) receptor ligand, specifically bound to rat macrophages (Kd = 0.75 +/- 0.19 nM, Bmax = 7.77 +/- 2.50 fmol/mg). RT-PCR experiments also showed the expression of endothelin ET(B) receptor mRNA, but not endothelin ET(A) receptor mRNA, in these macrophages. These results indicate that rat peritoneal macrophages apparently express the endothelin ET(B) receptor but not the endothelin ET(A) receptor.
authors.affiliation: Department of Pharmacology, Nagasaki University School of Medicine, Japan. yasukosy@net.nagasaki-u.ac.jp
authors.forename: Y
authors.initials: Y
authors.lastname: Sakurai-Yamashita
authors.forename: K
authors.initials: K
authors.lastname: Yamashita
authors.forename: A
authors.initials: A
authors.lastname: Yoshida
authors.forename: M
authors.initials: M
authors.lastname: Obana
authors.forename: K
authors.initials: K
authors.lastname: Takada
authors.forename: H
authors.initials: H
authors.lastname: Shibaguchi
authors.forename: K
authors.initials: K
authors.lastname: Shigematsu
authors.forename: M
authors.initials: M
authors.lastname: Niwa
authors.forename: K
authors.initials: K
authors.lastname: Taniyama
mesh_terms: D000818:Animals; D001345:Autoradiography; D066298:In Vitro Techniques; D017737:Macrophages, Peritoneal / Q000378:metabolism*; D008297:Male; D016133:Polymerase Chain Reaction; D012313:RNA / Q000096:biosynthesis / Q000302:isolation & purification; D051381:Rats; D017208:Rats, Wistar; D017466:Receptors, Endothelin / Q000096:biosynthesis*
chemical_list: D017466:Receptors, Endothelin; D012313:RNA