title: Specific monoclonal antibody raised against the p33ING1 tumor suppressor.
abstract: An IgG1 mouse monoclonal antibody (CAb1) was produced against human recombinant p33ING1. The antibody is able to recognize native and denatured antigen in ELISA and Western blot protocols, respectively. CAb1 can be used to specifically detect the p33ING1 protein in dot blot and Western immunoblot protocols of both human and mouse cell lysates. In addition, this antibody is also useful for cellular localization of native and ectopically overexpressed p33ING1 protein by indirect immunofluorescence.
authors.affiliation: Department of Medical Biochemistry, University of Calgary, Alberta, Canada.
authors.forename: I
authors.initials: I
authors.lastname: Garkavtsev
authors.forename: D
authors.initials: D
authors.lastname: Boland
authors.forename: J
authors.initials: J
authors.lastname: Mai
authors.forename: H
authors.initials: H
authors.lastname: Wilson
authors.forename: C
authors.initials: C
authors.lastname: Veillette
authors.forename: K
authors.initials: K
authors.lastname: Riabowol
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000096:biosynthesis / Q000276:immunology / Q000302:isolation & purification*; D015153:Blotting, Western; D018797:Cell Cycle Proteins; D004268:DNA-Binding Proteins; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D019084:Fluorescent Antibody Technique, Indirect; D016147:Genes, Tumor Suppressor; D006131:Growth Inhibitors / Q000276:immunology*; D006801:Humans; D000076023:Inhibitor of Growth Protein 1; D047908:Intracellular Signaling Peptides and Proteins; D051379:Mice; D008807:Mice, Inbred BALB C; D009687:Nuclear Proteins; D011506:Proteins / Q000276:immunology*; D025521:Tumor Suppressor Proteins
chemical_list: D000911:Antibodies, Monoclonal; D018797:Cell Cycle Proteins; D004268:DNA-Binding Proteins; D006131:Growth Inhibitors; C501503:ING1 protein, human; C501505:Ing1 protein, mouse; D000076023:Inhibitor of Growth Protein 1; D047908:Intracellular Signaling Peptides and Proteins; D009687:Nuclear Proteins; D011506:Proteins; D025521:Tumor Suppressor Proteins