Thy-1 is a component common to multiple populations of synaptic vesicles.
Thy-1, a glycosylphosphatidylinositol-linked integral membrane protein of the immunoglobulin superfamily, is a component of both large dense-core and small clear vesicles in PC12 cells. A majority of this protein, formerly recognized only on the plasma membrane of neurons, is localized to regulated secretory vesicles. Thy-1 is also present in synaptic vesicles in rat central nervous system. Experiments on permeabilized PC12 cells demonstrate that antibodies against Thy-1 inhibit the regulated release of neurotransmitter; this inhibition appears to be independent of any effect on the Ca2+ channel. These findings suggest Thy-1 is an integral component of many types of regulated secretory vesicles, and plays an important role in the regulated vesicular release of neurotransmitter at the synapse.
Jeng
C J
CJ
Department of Neurobiology and Brain Research Institute, UCLA School of Medicine, Los Angeles, California 90095-1763, USA.
McCarroll
S A
SA
Martin
T F
TF
Floor
E
E
Adams
J
J
Krantz
D
D
Butz
S
S
Edwards
R
R
Schweitzer
E S
ES
D000818:Animals; D015153:Blotting, Western; D001923:Brain Chemistry; D015220:Calcium Channels / Q000502:physiology; D002462:Cell Membrane / Q000737:chemistry; D005455:Fluorescent Antibody Technique; D007150:Immunohistochemistry; D009638:Norepinephrine / Q000378:metabolism; D016716:PC12 Cells; D051381:Rats; D013572:Synaptic Vesicles / Q000737:chemistry* / Q000378:metabolism; D018800:Thy-1 Antigens / Q000032:analysis* / Q000276:immunology / Q000502:physiology
D015220:Calcium Channels; D018800:Thy-1 Antigens; D009638:Norepinephrine