Molecular cloning of CISH, chromosome assignment to 3p21.3, and analysis of expression in fetal and adult tissues.
The murine cytokine inducible SH2-containing (Cis) protein gene (Cish) was recently cloned and shown to have a growth inhibitory function. We have isolated cDNAs coding for its human homologue (CISH) and assigned the gene to 3p21.3 by fluorescence in situ hybridization. Northern blot analysis showed CISH expression in various epithelial tissues including lung and kidney, in which tumors frequently exhibit 3p21.3 deletions.
Uchida
K
K
Laboratory of Ultrastructure Research, Aichi Cancer Center Research Institute, Nagoya, Japan.
Yoshimura
A
A
Inazawa
J
J
Yanagisawa
K
K
Osada
H
H
Masuda
A
A
Saito
T
T
Takahashi
T
T
Miyajima
A
A
Takahashi
T
T
D000328:Adult; D000595:Amino Acid Sequence; D001483:Base Sequence; D002893:Chromosomes, Human, Pair 3; D003001:Cloning, Molecular; D016207:Cytokines / Q000235:genetics*; D018076:DNA, Complementary; D005333:Fetus; D018507:Gene Expression Regulation, Developmental; D006801:Humans; D017874:Immediate-Early Proteins / Q000235:genetics*; D017404:In Situ Hybridization, Fluorescence; D008175:Lung Neoplasms / Q000235:genetics; D008969:Molecular Sequence Data; D017422:Sequence Analysis, DNA; D050826:Suppressor of Cytokine Signaling Proteins; D018909:src Homology Domains / Q000235:genetics*
D016207:Cytokines; D018076:DNA, Complementary; D017874:Immediate-Early Proteins; D050826:Suppressor of Cytokine Signaling Proteins