[Immunoassays for determination of glycoprotein specificity, serum quantitation and determination of binding sites of granulocyte antibodies].
For the characterization of granulocyte antibodies an antigen capture assay and a method based upon quantitative determination of eluted IgG were developed. These assays allowed the quantitation of granulocyte antibodies in sera, the determination of their binding sites and their glycoprotein specificity, and the identification of NA-specific antibodies in sera containing mixtures of granulocyte antibodies.
Bux
J
J
Institut für Klinische Immunologie und Transfusionsmedizin, Justus-Liebig-Universität Giessen, Deutschland.
Kober
B
B
Sohn
M
M
Mueller-Eckhardt
C
C
D000911:Antibodies, Monoclonal; D000918:Antibody Specificity; D015703:Antigens, CD / Q000097:blood; D001666:Binding Sites, Antibody; D018821:CD18 Antigens / Q000097:blood*; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D006098:Granulocytes / Q000276:immunology*; D006801:Humans; D007518:Isoantibodies / Q000097:blood*; D016177:Macrophage-1 Antigen / Q000097:blood*; D008562:Membrane Glycoproteins / Q000097:blood* / Q000276:immunology; D017452:Receptors, IgG / Q000032:analysis
D000911:Antibodies, Monoclonal; D015703:Antigens, CD; D018821:CD18 Antigens; D007518:Isoantibodies; D016177:Macrophage-1 Antigen; D008562:Membrane Glycoproteins; D017452:Receptors, IgG
Immunoassays zur Bestimmung der Glykoproteinspezifität, des Serumgehalts und der Bindungsstellenzahl von granulozytären Antikörpern.