Molecular cloning and localization of human syntaxin 16, a member of the syntaxin family of SNARE proteins.
We have cloned a new member of the syntaxin family of proteins, designated human syntaxin 16 (hsyn16). The open reading frame encodes a polypeptide of 307 amino acids with potential coiled-coil domains and a carboxy-terminal hydrophobic tail, which is characteristic of other members of the syntaxin family. The encoded polypeptide bears sequence homology to known syntaxin molecules. Northern blot analysis revealed a single transcript that is fairly ubiquitous, being slightly more enriched in heart and pancreas. Indirect immunofluorescence localised myc-tagged hsyn16 (myc-hsyn16) to the Golgi apparatus, colocalizing well with lens culinaris agglutinin, an established Golgi marker, as well as with other Golgi SNAREs such as GS28 and syntaxin 5. Myc-hsyn16 is redistributed to the endoplasmic reticulum upon brefeldin A treatment, indicating that it is localised to the Golgi stack. The ubiquitous expression and Golgi localization of hsy16 suggest that it is involved in a vesicular transport step within the organelle.
Tang
B L
BL
Membrane Biology Laboratory, Institute of Molecular and Cell Biology, Singapore, Republic of Singapore.
Low
D Y
DY
Lee
S S
SS
Tan
A E
AE
Hong
W
W
D000595:Amino Acid Sequence; D001483:Base Sequence; D015152:Blotting, Northern; D020126:Brefeldin A; D003001:Cloning, Molecular; D003517:Cyclopentanes / Q000494:pharmacology; D005455:Fluorescent Antibody Technique; D015870:Gene Expression / Q000235:genetics; D006056:Golgi Apparatus / Q000737:chemistry; D006801:Humans; D008565:Membrane Proteins / Q000737:chemistry*; D008856:Microscopy, Fluorescence; D008969:Molecular Sequence Data; D009419:Nerve Tissue Proteins / Q000737:chemistry; D050765:Qa-SNARE Proteins; D012333:RNA, Messenger / Q000032:analysis; D050600:SNARE Proteins; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D051438:Syntaxin 16; D033921:Vesicular Transport Proteins
D003517:Cyclopentanes; D008565:Membrane Proteins; D009419:Nerve Tissue Proteins; D050765:Qa-SNARE Proteins; D012333:RNA, Messenger; D050600:SNARE Proteins; D051438:Syntaxin 16; D033921:Vesicular Transport Proteins; D020126:Brefeldin A