T4 DNA ligase reduces chromosome damage and enhances cell survival in CHO cells treated with bleomycin.
The effect of DNA ligase from T4 phage on the modulation of the damage induced by bleomycin was studied. CHO6 cells were electroporated with either bleomycin alone or bleomycin plus T4 DNA ligase, and both cytogenetic and clonogenic assays were carried out in parallel. Our results show that T4 DNA ligase is able to decrease the frequency of chromosome aberrations in bleomycin-treated cells while increasing cell survival.
Ortiz
T
T
Department of Cell Biology, University of Seville, Spain.
Flores
M J
MJ
Piñero
J
J
Cortés
F
F
D000818:Animals; D000964:Antimetabolites, Antineoplastic / Q000494:pharmacology*; D001761:Bleomycin / Q000494:pharmacology*; D016466:CHO Cells / Q000166:cytology / Q000502:physiology; D002470:Cell Survival / Q000187:drug effects / Q000235:genetics; D006224:Cricetinae; D004249:DNA Damage / Q000187:drug effects*; D011088:DNA Ligases / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D018274:Electroporation
D000964:Antimetabolites, Antineoplastic; D001761:Bleomycin; D011088:DNA Ligases