High-performance liquid chromatographic separation of enantiomers of unusual amino acids on a teicoplanin chiral stationary phase.
A glycopeptide antibiotic, teicoplanin, was used as chiral stationary phase for the high-performance liquid chromatographic (HPLC) separation of enantiomers of more than 30 unnatural amino acids, such as phenylalanine and tyrosine analogues and analogues containing 1,2,3,4-tetrahydroisoquinoline, tetraline, 1,2,3,4-tetrahydro-2-carboline, cyclopentane, cyclohexane, cyclohexene, bicyclo[2.2.1]heptane or heptene skeletons. Excellent resolutions were achieved for most of the investigated compounds by using a hydro-organic mobile-phase system. The effects of organic modifier content, temperature and flow-rate on the resolution were investigated and the conditions of separation were optimized.
Péter
A
A
Department of Inorganic and Analytical Chemistry, Attila József University, Szeged, Hungary.
Török
G
G
Armstrong
D W
DW
D000596:Amino Acids / Q000302:isolation & purification*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D007536:Isomerism; D015394:Molecular Structure; D010649:Phenylalanine / Q000031:analogs & derivatives / Q000302:isolation & purification; D012997:Solvents; D017334:Teicoplanin / Q000378:metabolism*; D013696:Temperature; D014443:Tyrosine / Q000031:analogs & derivatives / Q000302:isolation & purification
D000596:Amino Acids; D012997:Solvents; D014443:Tyrosine; D010649:Phenylalanine; D017334:Teicoplanin