title: Analytical performance and clinical usefulness of two binding assays for growth hormone binding protein (GHBP) measurement: high performance liquid chromatography (HPLC)-gel filtration and dextran-coated charcoal adsorption.
abstract: We compared two binding assays for growth hormone binding protein (GHBP) measurements, which differ in the method of bound and free GH separation: HPLC-gel filtration or dextran coated-charcoal adsorption (DCC). Two pools of sera (high and medium GHBP activity) were used for quality-control assessment. Moreover, 62 samples from 34 children and 28 adults with different nutritional status were studied. Total, between- and intra-iodination coefficients of variation (CVs) from the two methods were not different. Although percentage binding measured in the pool sera significantly differed, the concentrations assessed by Scatchard plot were comparable. Results obtained by the two methods in the 62 sera were significantly correlated (r = 0.77, P < 0.001). With both methods GHBP activity correlated with chronological age and body mass index (BMI) and differed among groups with different nutritional status. Although HPLC and DCC separation methods for GHBP measurement differ in their practicability, our study demonstrates that performance and the clinical usefulness of the two methods are comparable.
authors.affiliation: Department of Clinical Biochemistry, Hospital Germans Trias i Pujol, Autonomous University of Barcelona, Spain.
authors.forename: M A
authors.initials: MA
authors.lastname: Llopis
authors.forename: M L
authors.initials: ML
authors.lastname: Granada
authors.forename: L
authors.initials: L
authors.lastname: Audí
authors.forename: A
authors.initials: A
authors.lastname: Sanmartí
authors.forename: J
authors.initials: J
authors.lastname: Bel
authors.forename: L
authors.initials: L
authors.lastname: Sánchez-Planell
authors.forename: X
authors.initials: X
authors.lastname: Formiguera
authors.forename: F
authors.initials: F
authors.lastname: Marin
authors.forename: A
authors.initials: A
authors.lastname: Corominas
mesh_terms: D000293:Adolescent; D000856:Anorexia Nervosa / Q000097:blood; D002352:Carrier Proteins / Q000097:blood*; D002606:Charcoal; D002648:Child; D002675:Child, Preschool; D002850:Chromatography, Gel; D002851:Chromatography, High Pressure Liquid; D003911:Dextrans; D005260:Female; D006130:Growth Disorders / Q000097:blood; D013006:Growth Hormone / Q000097:blood*; D006801:Humans; D007700:Kinetics; D008297:Male; D009747:Nutritional Physiological Phenomena; D009767:Obesity, Morbid / Q000097:blood; D011786:Quality Control
chemical_list: D002352:Carrier Proteins; D003911:Dextrans; D002606:Charcoal; D013006:Growth Hormone; C051815:somatotropin-binding protein