Thyroid stimulating hormone assays based on the detection of gold conjugates by scanning force microscopy.
Low bulk concentrations of thyroid stimulating hormone (TSH) were detected by scanning force microscopy (SFM) using gold-labeled conjugates. Anti-TSH antibodies were covalently bound onto amino-modified silicon oxide wafers. Surface modification was examined by contact-angle measurements, ellipsometry, X-ray photoelectron spectroscopy, and SFM. Antibodies were found to form a monolayer of prone molecules with an average surface density of 5000 IgG/mum2. TSH molecules were then allowed to bind to immobilized antibodies. The immunological reaction was quantified by SFM using gold-labeled species. Two scanned force microscopic immunoassays (SFMIA) were compared: first, a competitive test which used gold-labeled TSH molecules mixed with free TSH antigens was performed . Afterward, a sandwich assay was carried out, using gold-labeled anti-TSH antibodies. This latter method was found to be far more sensitive than competitive SFMIA. Gold conjugates were also found to be of great use to quantify antigens in large volumes by a sandwich test: a sensitivity threshold as low as 0.015 ng of TSH/ml (0.075 UI/ml or 6 x 10(-13) M) was estimated.
Perrin
A
A
Unité mixte CNRS/bioMérieux, Ecole normale supérieure de Lyon, France.
Theretz
A
A
Mandrand
B
B
D001667:Binding, Competitive / Q000276:immunology; D015374:Biosensing Techniques; D017979:Gold Colloid / Q000032:analysis*; D006801:Humans; D007118:Immunoassay / Q000295:instrumentation / Q000379:methods; D018625:Microscopy, Atomic Force / Q000295:instrumentation / Q000379:methods*; D011485:Protein Binding / Q000276:immunology; D012680:Sensitivity and Specificity; D012821:Silanes; D013499:Surface Properties; D013972:Thyrotropin / Q000032:analysis* / Q000276:immunology / Q000378:metabolism
D017979:Gold Colloid; D012821:Silanes; D013972:Thyrotropin