Base-pair mutation caused by four nitro-group containing aromatic amines in Salmonella typhimurium TA100, TA104, TA4001 and TA4006.
Among p-phenylenediamine, benzidine and the analogues we previously tested, only the nitro-group containing 2-nitro-p-phenylenediamine, 3-nitro-o-phenylenediamine, 4-nitro-o-phenylenediamine and 4,4'-dinitro-2-biphenylamine caused base-pair reversion in the histidine locus of Salmonella typhimurium TA100. In order to determine the types of mutations involved, such as transversion or transition, these four nitro-group containing compounds were tested with S. typhimurium strains TA100, TA104, TA4001 and TA4006. Dose-mutagenicity relationships occurred with TA100 and TA104. However, the majority of revertants from TA100 and TA104 were insensitive to inhibition by histidine analogue, DL-1,2,4-triazole-3-alanine. These results suggested that the occurrence of histidine revertants was predominantly induced by base-pair (point) mutations and not by suppressor gene mutations. The CG-TA transition and CG-AT transversion are the major types of mutation induced by all these compounds in TA100. The TA-AT transversion also contributed to the mutagenicity of 4-nitro-o-phenylenediamine and 4,4'-dinitro-2-biphenylamine in TA104. These nitro-group containing compounds showed no mutagenicity in TA4001, but induced CG-GC transversion in TA4006.
Chen
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SC
Department of Microbiology and Molecular Cell Science, University of Memphis, TN 38152, USA.
Wong
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TY
Chung
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KT
D000611:Aminobiphenyl Compounds / Q000737:chemistry / Q000633:toxicity*; D001482:Base Composition; D004305:Dose-Response Relationship, Drug; D016153:Genes, Suppressor; D009152:Mutagenicity Tests; D009153:Mutagens / Q000737:chemistry / Q000633:toxicity; D009154:Mutation; D009574:Nitro Compounds / Q000737:chemistry / Q000633:toxicity*; D010655:Phenylenediamines / Q000737:chemistry / Q000633:toxicity*; D011765:Pyruvaldehyde / Q000633:toxicity; D012486:Salmonella typhimurium / Q000187:drug effects / Q000235:genetics*; D013329:Structure-Activity Relationship
C110699:4,4'-dinitro-2-biphenylamine; D000611:Aminobiphenyl Compounds; D009153:Mutagens; D009574:Nitro Compounds; D010655:Phenylenediamines; C012277:1,2-diamino-4-nitrobenzene; D011765:Pyruvaldehyde; C014706:2-nitro-4-phenylenediamine; C110700:3-nitro-o-phenylenediamine