title: Gene arrangement in the upstream region of Clostridium botulinum type E and Clostridium butyricum BL6340 progenitor toxin genes is different from that of other types.
abstract: The cluster of genes encoding the botulinum progenitor toxin and the upstream region including p21 and p47 were divided into three different gene arrangements (class I-III). To determine the gene similarity of the type E neurotoxin (BoNT/E) complex to other types, the gene organization in the upstream region of the nontoxic-nonhemagglutinin gene (ntnh) was investigated in chromosomal DNA from Clostridium botulinum type E strain Iwanai and C. butyricum strain BL6340. The gene cluster of type E progenitor toxin (Iwanai and BL6340) was similar to those of type F and type A (from infant botulism in Japan), but not to those of types A, B, and C. Though genes for the hemagglutinin component and P21 were not discovered, genes encoding P47, NTNH, and BoNT were found in type E strain Iwanai and C. butyricum strain BL6340. However, the genes of ORF-X1 (435 bp) and ORF-X2 (partially sequenced) were present just upstream of that of P47. The orientation of these genes was in inverted direction to that of p47. The gene cluster of type E progenitor toxin (Iwanai and BL6340) is, therefore, a specific arrangement (class IV) among the genes encoding components of the BoNT complex.
authors.affiliation: Department of Microbiology, Sapporo Medical University School of Medicine, Hokkaido, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Kubota
authors.forename: N
authors.initials: N
authors.lastname: Yonekura
authors.forename: Y
authors.initials: Y
authors.lastname: Hariya
authors.forename: E
authors.initials: E
authors.lastname: Isogai
authors.forename: H
authors.initials: H
authors.lastname: Isogai
authors.forename: K
authors.initials: K
authors.lastname: Amano
authors.forename: N
authors.initials: N
authors.lastname: Fujii
mesh_terms: D001905:Botulinum Toxins / Q000235:genetics*; D003001:Cloning, Molecular; D003014:Clostridium botulinum / Q000235:genetics*; D015964:Gene Expression Regulation, Bacterial; D015321:Gene Rearrangement; D005798:Genes, Bacterial / Q000235:genetics; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D012045:Regulatory Sequences, Nucleic Acid / Q000235:genetics*; D017386:Sequence Homology, Amino Acid; D013045:Species Specificity
chemical_list: D001905:Botulinum Toxins; C096326:botulinum toxin type E