Characterization of the germinal and somatic activity of the Arabidopsis transposable element Tag1.
Tag1 is an autonomous transposon of Arabidopsis thaliana. The excision behavior of Tag1 during reproductive and vegetative development was examined using CaMV 35STag1-GUS constructs. Germinal reversion frequencies varied from 0 to 27% and correlated with Tag1 copy number. Southern blot and somatic sector analyses indicated that each revertant was derived from an independent excision event, and approximately 75% of the revertants had new Tag1 insertions. Revertants were obtained with similar frequencies from the male and female parents. In flowers, small somatic sectors were observed in siliques, carpels, petals and sepals while stemlike organs (filaments and pedicels) had larger sectors. No sectors encompassing entire flowers or inflorescences were observed, however, indicating that excision occurs late in flower development and rarely in inflorescence meristems. Late excision was also observed during vegetative development with 99.8% of leaves showing small sectors encompassing no more than 20 cells. Roots and cotyledons, however, showed larger sectors that included entire lateral roots and cotyledons. These results indicate that Tag1 can excise in the embryo and all the organs of the plant with the timing of excision being restricted to late stages of vegetative and reproductive development in the shoot.
Liu
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Department of Biology and Center for Molecular Genetics, University of California at San Diego, La Jolla 92093, USA.
Crawford
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D017360:Arabidopsis / Q000235:genetics* / Q000502:physiology; D004251:DNA Transposable Elements / Q000235:genetics* / Q000502:physiology; D018744:DNA, Plant / Q000235:genetics*
D004251:DNA Transposable Elements; D018744:DNA, Plant