title: Structure of Sesbania mosaic virus at 3 A resolution.
abstract: Sesbania mosaic virus (SMV) is an isometric, ss-RNA plant virus found infecting Sesbania grandiflora plants in fields near Tirupathi, South India. The virus particles, which sediment at 116 S at pH 5.5, swell upon treatment with EDTA at pH 7.5 resulting in the reduction of the sedimentation coefficient to 108 S. SMV coat protein amino acid sequence was determined and found to have approximately 60% amino acid sequence identity with that of southern bean mosaic virus (SBMV). The amino terminal 60 residue segment, which contains a number of positively charged residues, is less well conserved between SMV and SBMV when compared to the rest of the sequence. The 3D structure of SMV was determined at 3.0 A resolution by molecular replacement techniques using SBMV structure as the initial phasing model. The icosahedral asymmetric unit was found to contain four calcium ions occurring in inter subunit interfaces and three protein subunits, designated A, B and C. The conformation of the C subunit appears to be different from those of A and B in several segments of the polypeptide. These observations coupled with structural studies on SMV partially depleted of calcium suggest a plausible mechanism for the initiation of the disassembly of the virus capsid.
authors.affiliation: Molecular Biophysics Unit, Indian Institute of Science, Bangalore, India.
authors.forename: M R
authors.initials: MR
authors.lastname: Murthy
authors.forename: M
authors.initials: M
authors.lastname: Bhuvaneswari
authors.forename: H S
authors.initials: HS
authors.lastname: Subramanya
authors.forename: K
authors.initials: K
authors.lastname: Gopinath
authors.forename: H S
authors.initials: HS
authors.lastname: Savithri
mesh_terms: D000595:Amino Acid Sequence; D001665:Binding Sites; D002118:Calcium / Q000378:metabolism; D002213:Capsid / Q000378:metabolism / Q000648:ultrastructure; D007887:Fabaceae / Q000821:virology*; D006361:Heating; D008969:Molecular Sequence Data; D009029:Mosaic Viruses / Q000502:physiology / Q000648:ultrastructure*; D010946:Plants, Medicinal; D011487:Protein Conformation; D012367:RNA, Viral / Q000648:ultrastructure; D017386:Sequence Homology, Amino Acid; D019065:Virus Assembly; D014961:X-Ray Diffraction
chemical_list: D012367:RNA, Viral; D002118:Calcium