Estradiol and fibulin-1 inhibit motility of human ovarian- and breast-cancer cells induced by fibronectin.
Ovarian-cancer cells are characterized by their ability to invade freely the peritoneal cavity. Estradiol stimulates the proliferation of estrogen-receptor(ER)-positive ovarian-cancer cells, as well as expression of fibulin-1, a fibronectin-binding extracellular matrix protein. Using a modified Boyden-chamber assay, we have evaluated the respective roles of estradiol and fibulin-1 on cell motility, one of the earlier steps of tumor invasion. The effect of estradiol was examined on the random and directional migration of different ER-positive ovarian-cancer cell lines. The effect of fibulin-1 was studied on the motility of the MDA-MB231 breast-cancer cell line, which does not express fibulin-1. We found that when fibronectin (FN) was used as an attractant, estradiol decreased the cell motility of 2 ER-positive ovarian-cancer cell lines, BG-1 and SKOV3, but had no effect on 2 ER-negative cell lines, PEO14 and MDA-MB231. The inhibitory effect of estradiol was not observed when collagen (type 1 or 4) or laminin were used as attractants. Fibulin-1 was found to inhibit haptotactic migration of MDA-MB231 cells to FN in a dose-dependent manner. We conclude that both estradiol and fibulin-1 inhibit cancer cell motility in vitro and therefore have the potential to inhibit tumor invasion.
Hayashido
Y
Y
Unité Hormones et Cancer (U 148) INSERM, Montpellier, France.
Lucas
A
A
Rougeot
C
C
Godyna
S
S
Argraves
W S
WS
Rochefort
H
H
D001943:Breast Neoplasms / Q000473:pathology; D002135:Calcium-Binding Proteins / Q000494:pharmacology*; D002465:Cell Movement / Q000187:drug effects*; D002633:Chemotaxis; D004958:Estradiol / Q000494:pharmacology*; D016326:Extracellular Matrix Proteins / Q000502:physiology; D005260:Female; D005353:Fibronectins / Q000494:pharmacology*; D006801:Humans; D009361:Neoplasm Invasiveness; D010051:Ovarian Neoplasms / Q000473:pathology*; D014407:Tumor Cells, Cultured
D002135:Calcium-Binding Proteins; D016326:Extracellular Matrix Proteins; D005353:Fibronectins; C060820:fibulin; D004958:Estradiol