Evaluation of the RapID CB Plus system for identification of Corynebacterium species and other gram-positive rods.
Due to the difficulty of identifying Corynebacterium spp. with standard methods, we compared them with the RapID CB Plus system (Remel, Lenexa, Kans. [formerly Innovative Diagnostic Systems, Norcross, Ga.]), which consists of 4 carbohydrate and 14 preformed enzyme tests, for the identification of 98 clinical isolates of Corynebacterium sp., other coryneforms, Listeria monocytogenes, and 17 ATCC strains. Forty (95%) of 42 strains of Corynebacterium spp. were accurately identified to the species level by the RapID CB Plus system, and two additional strains of C. striatum were identified with one additional conventional test for lipid requirement. Twenty-seven (75%) of the 36 coryneform strains tested were identified correctly to the species level. However, three of four strains of Brevibacterium sp. and all seven of the L. monocytogenes strains were identified to the genus level only. Actinomyces strains had variable results, and the one strain of Arcanobacterium haemolyticum tested was not identified. Overall, the RapID CB Plus system compared favorably with the conventional methods, was easy to inoculate and interpret, and is promising as a new method for identification of gram-positive bacilli.
Hudspeth
M K
MK
R. M. Alden Research Laboratory, Santa Monica-UCLA Medical Center, California 90404, USA.
Hunt Gerardo
S
S
Citron
D M
DM
Goldstein
E J
EJ
D000190:Actinomyces / Q000201:enzymology / Q000302:isolation & purification / Q000378:metabolism; D000626:Aminopeptidases / Q000378:metabolism; D001431:Bacteriological Techniques; D001951:Brevibacterium / Q000201:enzymology / Q000302:isolation & purification / Q000378:metabolism; D050260:Carbohydrate Metabolism; D003352:Corynebacterium / Q000201:enzymology / Q000302:isolation & purification* / Q000378:metabolism; D003354:Corynebacterium Infections / Q000175:diagnosis* / Q000201:enzymology / Q000378:metabolism; D003470:Culture Media / Q000378:metabolism; D004798:Enzymes / Q000378:metabolism*; D006026:Glycoside Hydrolases / Q000378:metabolism; D016908:Gram-Positive Bacterial Infections / Q000175:diagnosis* / Q000201:enzymology / Q000378:metabolism; D016987:Gram-Positive Rods / Q000201:enzymology / Q000302:isolation & purification* / Q000378:metabolism; D006801:Humans; D050356:Lipid Metabolism; D008089:Listeria monocytogenes / Q000201:enzymology / Q000302:isolation & purification / Q000378:metabolism; D011933:Reagent Kits, Diagnostic
D003470:Culture Media; D004798:Enzymes; D011933:Reagent Kits, Diagnostic; D006026:Glycoside Hydrolases; D000626:Aminopeptidases