title: Protein binding regions of the mRNAs for the 55 kDa tumor necrosis factor receptor and the glucose transporter 1: sequence homology and competition for cellular proteins.
abstract: Gene expression is influenced by mechanisms regulating mRNA degradation. Knowledge on regulatory RNA elements involved and on proteins interacting with them is still limited. A 33 nucleotide (nt) region of the 55 kDa tumor necrosis factor receptor (TNFR-55) mRNA, previously reported by us to engage in such interaction with proteins from U-937 cells, exhibits homology to a 38 nt regulatory region of the glucose transporter GLUT-1 mRNA. Labeled RNA fragments comprising these two regions bind similar sets of proteins. Upon phorbol ester-induced differentiation into macrophage-like cells, protein binding to both fragments is changed similarly. Furthermore, both compete with each other for protein binding. This suggests that GLUT-1 and TNFR-55 RNA share a novel protein binding RNA motif involved in regulation of their half life.
authors.affiliation: Institute of Molecular Pharmacology, Medical School Hannover, Germany.
authors.forename: S
authors.initials: S
authors.lastname: Kafert
authors.forename: R
authors.initials: R
authors.lastname: Winzen
authors.forename: A
authors.initials: A
authors.lastname: Loos
authors.forename: F
authors.initials: F
authors.lastname: Bollig
authors.forename: K
authors.initials: K
authors.lastname: Resch
authors.forename: H
authors.initials: H
authors.lastname: Holtmann
mesh_terms: D000818:Animals; D015703:Antigens, CD / Q000096:biosynthesis*; D001483:Base Sequence; D001665:Binding Sites; D002417:Cattle; D002454:Cell Differentiation; D017931:DNA Primers; D051272:Glucose Transporter Type 1; D006801:Humans; D016403:Lymphoma, Large B-Cell, Diffuse; D008264:Macrophages / Q000378:metabolism; D008297:Male; D009004:Monosaccharide Transport Proteins / Q000096:biosynthesis*; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000737:chemistry* / Q000378:metabolism*; D016601:RNA-Binding Proteins / Q000378:metabolism*; D018124:Receptors, Tumor Necrosis Factor / Q000096:biosynthesis*; D047888:Receptors, Tumor Necrosis Factor, Type I; D012045:Regulatory Sequences, Nucleic Acid; D016415:Sequence Alignment; D012689:Sequence Homology, Nucleic Acid; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology; D014158:Transcription, Genetic; D014407:Tumor Cells, Cultured
chemical_list: D015703:Antigens, CD; D017931:DNA Primers; D051272:Glucose Transporter Type 1; D009004:Monosaccharide Transport Proteins; D012333:RNA, Messenger; D016601:RNA-Binding Proteins; D018124:Receptors, Tumor Necrosis Factor; D047888:Receptors, Tumor Necrosis Factor, Type I; C495677:SLC2A1 protein, human; D013755:Tetradecanoylphorbol Acetate