title: Immunocytochemical localization of the mGluR1b metabotropic glutamate receptor in the rat hypothalamus.
abstract: The mGluR1 metabotropic glutamate receptor is a G-protein-coupled receptor that exists as different C-terminal splice variants. When expressed in mammalian cells, the mGluR1 splice variants exhibit diverse transduction mechanisms and also slightly differ in their apparent agonist affinities. In the present study, we used an affinity-purified antiserum, specifically reactive to the mGluRlb splice variant, in combination with a highly sensitive preembedding immunocytochemical method for light microscopy to investigate the distribution of this receptor in the rat hypothalamus. An intense immunoreactivity for mGluRlb was observed in distinct hypothalamic nuclei. Thus, neuronal cell bodies and dendrites were stained in the preoptic area, suprachiasmatic nucleus, dorsal hypothalamus, lateral hypothalamus, dorsomedial nucleus, tuberomammilary nucleus, and lateral mammilary body. The ventromedial nucleus exhibited neuropil immunostaining but neuronal cell bodies were not labeled. Strong mGluRlb immunoreactivity was observed in magnocellular neurons of the neuroendocrine supraoptic, paraventricular, and arcuate nuclei. Also, neuronal cell bodies were heavily labeled in the retrochiasmatic nucleus, anterior commissural nucleus, and periventricular nucleus. These immunocytochemical observations, together with previous studies, suggest that mGluRlb is coexpressed with other class I mGluRs in some nuclei throughout the hypothalamus. However, mGluRlb is so far the only receptor of this class strongly expressed in the supraoptic, paraventricular, and arcuate nuclei, which might have relevant implications in the physiological control of the neuroendocrine hypothalamic-pituitary system.
authors.affiliation: Department of Neurosciences, Faculty of Medicine and Dentistry, Basque Country University, Bilbao, Spain.
authors.forename: J M
authors.initials: JM
authors.lastname: Mateos
authors.forename: J
authors.initials: J
authors.lastname: Azkue
authors.forename: R
authors.initials: R
authors.lastname: Benítez
authors.forename: R
authors.initials: R
authors.lastname: Sarría
authors.forename: J
authors.initials: J
authors.lastname: Losada
authors.forename: F
authors.initials: F
authors.lastname: Conquet
authors.forename: F
authors.initials: F
authors.lastname: Ferraguti
authors.forename: R
authors.initials: R
authors.lastname: Kuhn
authors.forename: T
authors.initials: T
authors.lastname: Knöpfel
authors.forename: P
authors.initials: P
authors.lastname: Grandes
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D007031:Hypothalamus / Q000737:chemistry*; D007106:Immune Sera; D007150:Immunohistochemistry; D008297:Male; D008969:Molecular Sequence Data; D012326:RNA Splicing; D051381:Rats; D017207:Rats, Sprague-Dawley; D018094:Receptors, Metabotropic Glutamate / Q000032:analysis*
chemical_list: D007106:Immune Sera; D018094:Receptors, Metabotropic Glutamate