title: Identification and characterization of a novel RING-finger gene (RNF4) mapping at 4p16.3.
abstract: We have isolated a new human RING-finger gene (RNF4) that encodes a 190-amino-acid protein. RNF4, in addition to the carboxyl-terminally located RING-finger motif, contains two putative nuclear localization signals and stretches of acidic amino acids that are similar to the activation domains of some transcription factors. RNF4 was expressed at low levels in all human tissues examined, with the notable exception of very high expression in the testis. The mouse homolog of RNF4 was abundantly expressed in embryonic tissues from the earliest days postgestation and exhibited a ubiquitous pattern of expression as assessed by in situ hybridization. We have mapped RNF4 to 4p16.3, a chromosome region associated with several genetic and neoplastic diseases. RNF4 spans 47 kb, is composed of eight exons, and maps immediately proximal to the anonymous locus D4S183, between the huntingtin (HD) and the fibroblast growth factor receptor 3 (FGFR3) genes.
authors.affiliation: Dipartimento di Biologia e Patologia Cellulare e Molecolare L. Califano, Università degli Studi di Napoli Federico II, Naples, Italy.
authors.forename: L
authors.initials: L
authors.lastname: Chiariotti
authors.forename: G
authors.initials: G
authors.lastname: Benvenuto
authors.forename: M
authors.initials: M
authors.lastname: Fedele
authors.forename: M
authors.initials: M
authors.lastname: Santoro
authors.forename: A
authors.initials: A
authors.lastname: Simeone
authors.forename: A
authors.initials: A
authors.lastname: Fusco
authors.forename: C B
authors.initials: CB
authors.lastname: Bruni
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002874:Chromosome Mapping; D002894:Chromosomes, Human, Pair 4 / Q000235:genetics*; D003001:Cloning, Molecular; D004268:DNA-Binding Proteins / Q000032:analysis / Q000737:chemistry / Q000235:genetics*; D018507:Gene Expression Regulation, Developmental; D006801:Humans; D051379:Mice; D008969:Molecular Sequence Data; D009687:Nuclear Proteins; D009928:Organ Specificity / Q000235:genetics; D014157:Transcription Factors; D044767:Ubiquitin-Protein Ligases; D016335:Zinc Fingers / Q000235:genetics*
chemical_list: D004268:DNA-Binding Proteins; D009687:Nuclear Proteins; C114070:RNF4 protein, human; D014157:Transcription Factors; C486410:Rnf4 protein, mouse; D044767:Ubiquitin-Protein Ligases