title: Thy-1 is a component common to multiple populations of synaptic vesicles.
abstract: Thy-1, a glycosylphosphatidylinositol-linked integral membrane protein of the immunoglobulin superfamily, is a component of both large dense-core and small clear vesicles in PC12 cells. A majority of this protein, formerly recognized only on the plasma membrane of neurons, is localized to regulated secretory vesicles. Thy-1 is also present in synaptic vesicles in rat central nervous system. Experiments on permeabilized PC12 cells demonstrate that antibodies against Thy-1 inhibit the regulated release of neurotransmitter; this inhibition appears to be independent of any effect on the Ca2+ channel. These findings suggest Thy-1 is an integral component of many types of regulated secretory vesicles, and plays an important role in the regulated vesicular release of neurotransmitter at the synapse.
authors.affiliation: Department of Neurobiology and Brain Research Institute, UCLA School of Medicine, Los Angeles, California 90095-1763, USA.
authors.forename: C J
authors.initials: CJ
authors.lastname: Jeng
authors.forename: S A
authors.initials: SA
authors.lastname: McCarroll
authors.forename: T F
authors.initials: TF
authors.lastname: Martin
authors.forename: E
authors.initials: E
authors.lastname: Floor
authors.forename: J
authors.initials: J
authors.lastname: Adams
authors.forename: D
authors.initials: D
authors.lastname: Krantz
authors.forename: S
authors.initials: S
authors.lastname: Butz
authors.forename: R
authors.initials: R
authors.lastname: Edwards
authors.forename: E S
authors.initials: ES
authors.lastname: Schweitzer
mesh_terms: D000818:Animals; D015153:Blotting, Western; D001923:Brain Chemistry; D015220:Calcium Channels / Q000502:physiology; D002462:Cell Membrane / Q000737:chemistry; D005455:Fluorescent Antibody Technique; D007150:Immunohistochemistry; D009638:Norepinephrine / Q000378:metabolism; D016716:PC12 Cells; D051381:Rats; D013572:Synaptic Vesicles / Q000737:chemistry* / Q000378:metabolism; D018800:Thy-1 Antigens / Q000032:analysis* / Q000276:immunology / Q000502:physiology
chemical_list: D015220:Calcium Channels; D018800:Thy-1 Antigens; D009638:Norepinephrine