title: Natural polymorphism in the thrombospondin-related adhesive protein of Plasmodium falciparum.
abstract: We have developed a typing system using natural sequence variation in the thrombospondin-related adhesive protein (TRAP) gene of Plasmodium falciparum. This method permits a haplotype to be assigned to any particular TRAP gene. We have applied this method to a hospital-based, case control-study in Mali. Previous sequence variation and conservation in TRAP has been confirmed. Particular TRAP haplotypes can be used as geographic hallmarks. Because of the high level of conflict between characters, we have examined the phylogenetic relationships between parasites using a network approach. Having received patient samples from urban and periurban areas of Bamako, the majority of haplotypes were closely related and distinct from TRAP sequences present in other continents. This suggests that the structure of TRAP can only tolerate a limited number of sequence variations to preserve its function but that this is sufficient to allow the parasite to evade the host's immune system until a long-lived immune response can be maintained. It may also reflect host genetics in that certain variants may escape the host immune response more efficiently than others. For vaccine design, sequences from the major regional variants may need to be considered in the production of effective subunit vaccines.
authors.affiliation: Medical Research Council Molecular Haematology Unit, Institute of Molecular Medicine, University of Oxford, Headington, United Kingdom.
authors.forename: K J
authors.initials: KJ
authors.lastname: Robson
authors.forename: A
authors.initials: A
authors.lastname: Dolo
authors.forename: I R
authors.initials: IR
authors.lastname: Hackford
authors.forename: O
authors.initials: O
authors.lastname: Doumbo
authors.forename: M B
authors.initials: MB
authors.lastname: Richards
authors.forename: M M
authors.initials: MM
authors.lastname: Keita
authors.forename: T
authors.initials: T
authors.lastname: Sidibe
authors.forename: A
authors.initials: A
authors.lastname: Bosman
authors.forename: D
authors.initials: D
authors.lastname: Modiano
authors.forename: A
authors.initials: A
authors.lastname: Crisanti
mesh_terms: D000818:Animals; D016022:Case-Control Studies; D002648:Child; D002675:Child, Preschool; D016054:DNA, Protozoan / Q000032:analysis* / Q000235:genetics; D017125:Genes, Protozoan; D006239:Haplotypes; D006454:Hemoglobins / Q000032:analysis; D006801:Humans; D007223:Infant; D007525:Isoelectric Focusing; D016778:Malaria, Falciparum / Q000453:epidemiology / Q000235:genetics*; D008302:Mali / Q000453:epidemiology; D017720:Molecular Epidemiology; D010802:Phylogeny; D010963:Plasmodium falciparum / Q000235:genetics*; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length; D015800:Protozoan Proteins / Q000235:genetics*; D012091:Repetitive Sequences, Nucleic Acid; D017422:Sequence Analysis, DNA
chemical_list: D016054:DNA, Protozoan; D006454:Hemoglobins; D015800:Protozoan Proteins; C069428:thrombospondin-related adhesive protein, protozoan