title: Clinical significance of neutrophil adhesion molecules expression after coronary angioplasty on the development of restenosis.
abstract: To investigate the neutrophil activation process following percutaneous transluminal coronary angioplasty (PTCA), we examined the expressions of Mac-1 (CD11b/CD18), L-selectin (CD62L), and sialyl-LewisX (SLX) on the surface of neutrophils after the PTCA procedure, by flow cytometric analysis. Twenty-nine patients with single vessel coronary artery disease of the left anterior descending artery who underwent elective PTCA were enrolled. In the 17 patients without restenosis at the follow-up angiography, the mean channel fluorescence intensity (MFI) for CD18, CD62L and SLX did not change after PTCA. Only the CD11b level was increased at 48 h after the PTCA. In the remaining 12 patients who developed restenosis, the MFI values for CD18 and CD11b were increased at 24 h and 48 h after the PTCA. The MFI value for CD62L was decreased and that for SLX was increased at 48 h after the PTCA. These changes were more prominent in the coronary sinus blood samples than in those of the peripheral blood samples. Our data indicate the down-regulation of L-selectin, probably by shedding, as well as the up-regulations of Mac-1 and sialyl-LewisX, especially in patients with restenosis. It is suggested that neutrophil activation by an interaction between the selectin family and carbohydrate ligands after PTCA may play a role in the development of restenosis, as does the integrin family.
authors.affiliation: Department of Cardiology, Koshigaya Hospital, Dokkyo University School of Medicine, Koshigaya City, Saitama, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Inoue
authors.forename: Y
authors.initials: Y
authors.lastname: Sakai
authors.forename: T
authors.initials: T
authors.lastname: Fujito
authors.forename: K
authors.initials: K
authors.lastname: Hoshi
authors.forename: T
authors.initials: T
authors.lastname: Hayashi
authors.forename: K
authors.initials: K
authors.lastname: Takayanagi
authors.forename: S
authors.initials: S
authors.lastname: Morooka
mesh_terms: D015906:Angioplasty, Balloon, Coronary; D000954:Antigens, Surface / Q000097:blood; D015815:Cell Adhesion Molecules / Q000032:analysis*; D003327:Coronary Disease / Q000097:blood*; D005260:Female; D006801:Humans; D019041:L-Selectin / Q000097:blood; D007983:Lewis Blood Group Antigens / Q000276:immunology; D016177:Macrophage-1 Antigen / Q000097:blood; D008297:Male; D008875:Middle Aged; D018375:Neutrophil Activation; D009844:Oligosaccharides / Q000097:blood; D012008:Recurrence; D000080506:Sialyl Lewis X Antigen
chemical_list: D000954:Antigens, Surface; D015815:Cell Adhesion Molecules; D007983:Lewis Blood Group Antigens; D016177:Macrophage-1 Antigen; D009844:Oligosaccharides; D000080506:Sialyl Lewis X Antigen; D019041:L-Selectin