title: Analysis of high density lipoprotein apolipoproteins by capillary zone and capillary SDS gel electrophoresis.
abstract: We describe new methods for analyzing the apolipoproteins (apo) of the high density lipoproteins (HDL) of several species by two modes of capillary electrophoresis: size separation using a molecular sieving buffer, and capillary zone electrophoresis (CZE) using neutral coated capillaries. By either mode HDL apos were resolved within 25 min. Results for apoA-I and apoA-II mass agreed with those by electroimmunoassay; intra-assay coefficients of variation were 1.8-4.2%. The migration times of human, rat, rabbit, and bovine apoA-I during CZE were proportional to their net charge/Mr ratios. This enabled human and rabbit apoA-I to be quantified simultaneously in transgenic rabbit HDLs. CZE also resolved human apoA-I isoforms, deamidated apoA-I, and pro-apoA-I.
authors.affiliation: Department of Cardiovascular Biochemistry, St. Bartholomew's and the Royal London School of Medicine and Dentistry, United Kingdom.
authors.forename: J
authors.initials: J
authors.lastname: Stocks
authors.forename: M N
authors.initials: MN
authors.lastname: Nanjee
authors.forename: N E
authors.initials: NE
authors.lastname: Miller
mesh_terms: D000818:Animals; D016632:Apolipoprotein A-I / Q000032:analysis / Q000737:chemistry; D016633:Apolipoprotein A-II / Q000032:analysis / Q000737:chemistry; D001053:Apolipoproteins / Q000032:analysis*; D002417:Cattle; D019075:Electrophoresis, Capillary / Q000379:methods*; D006801:Humans; D007118:Immunoassay; D008075:Lipoproteins, HDL / Q000032:analysis*; D011817:Rabbits; D051381:Rats; D012967:Sodium Dodecyl Sulfate
chemical_list: D016632:Apolipoprotein A-I; D016633:Apolipoprotein A-II; D001053:Apolipoproteins; D008075:Lipoproteins, HDL; D012967:Sodium Dodecyl Sulfate