Lung function in premature lambs and rabbits treated with a recombinant SP-C surfactant.
We evaluated the efficacy of a recombinant surfactant protein-C (rSP-C) surfactant in vitro and in vivo, using ventilated preterm lambs and rabbits. The surface tension measured with a Wilhelmy balance of the rSP-C surfactant was lower than the surface tension of natural sheep surfactant (containing SP-B and SP-C). The rSP-C and natural surfactants were equivalently sensitive to inhibition by plasma. Over a 5-h study period, lambs treated with 100 mg/kg rSP-C and natural surfactants at birth had similar PCO2, lung mechanics, and FRC values. Indicators of injury also were not different. In preterm rabbits ventilated with 3 cm H2O positive end-expiratory pressure (PEEP), similar compliance responses were measured for the rSP-C and natural surfactant. Large-aggregate surfactants recovered from alveolar washes of the lambs treated with rSP-C or natural surfactant retained their function when tested in preterm rabbits. In conclusion, the rSP-C surfactant was effective in preterm animals with surfactant deficiency.
Davis
A J
AJ
Department of Pediatrics, Harbor-UCLA Medical Center, UCLA School of Medicine, Torrance, California, USA.
Jobe
A H
AH
Häfner
D
D
Ikegami
M
M
D000818:Animals; D000831:Animals, Newborn / Q000502:physiology*; D005865:Gestational Age; D008168:Lung / Q000187:drug effects* / Q000502:physiology*; D008170:Lung Compliance / Q000187:drug effects; D011175:Positive-Pressure Respiration; D011510:Proteolipids / Q000494:pharmacology*; D011663:Pulmonary Surfactants / Q000494:pharmacology*; D011817:Rabbits; D011994:Recombinant Proteins; D012121:Respiration, Artificial; D012756:Sheep; D013500:Surface Tension
D011510:Proteolipids; D011663:Pulmonary Surfactants; D011994:Recombinant Proteins