Expression of CD44 splicing isoforms in lung cancers: dominant expression of CD44v8-10 in non-small cell lung carcinomas.
We examined CD44 isoform expression in 138 frozen tissue samples, which included primary lung carcinomas, adjacent non-tumorous lung tissues and benign lung diseases, by both reverse transcriptase polymerase chain reaction (RT-PCR) and immunohistochemical analyses. CD44v8-10 mRNA and protein were dominantly expressed in non-small cell lung carcinomas (NSCLC), while non-tumorous tissues principally expressed CD44s and small cell lung carcinomas (SCLC) expressed either CD44s or no detectable CD44. These results indicate that CD44v8-10 is the dominant splicing isoform in NSCLC and can be practically utilized as a diagnostic marker and therapeutical target in NSCLC.
Sasaki
J I
JI
Department of Tumor Genetics, Kumamoto University School of Medicine, 2-2-1 Honjo, Kumamoto 860, Japan.
Tanabe
K K
KK
Takahashi
K
K
Okamoto
I
I
Fujimoto
H
H
Matsumoto
M
M
Suga
M
M
Ando
M
M
Saya
H
H
D000368:Aged; D017398:Alternative Splicing; D015703:Antigens, CD / Q000032:analysis / Q000096:biosynthesis*; D002289:Carcinoma, Non-Small-Cell Lung / Q000276:immunology / Q000473:pathology*; D018288:Carcinoma, Small Cell / Q000276:immunology / Q000473:pathology; D005260:Female; D006801:Humans; D018960:Hyaluronan Receptors / Q000032:analysis / Q000096:biosynthesis*; D007150:Immunohistochemistry; D008171:Lung Diseases / Q000276:immunology / Q000473:pathology; D008175:Lung Neoplasms / Q000276:immunology / Q000473:pathology*; D008297:Male; D008875:Middle Aged; D009362:Neoplasm Metastasis; D016133:Polymerase Chain Reaction; D012907:Smoking; D014407:Tumor Cells, Cultured
D015703:Antigens, CD; D018960:Hyaluronan Receptors