Construction of a sorbitol-based vector for expression of heterologous proteins in Saccharomyces cerevisiae.
A new inducible yeast expression vector, pXS7, was constructed by using the promoter and terminator sequences from the Saccharomyces cerevisiae SOR1 gene, which codes for the sorbitol dehydrogenase protein. We cloned the coding sequence of the Saccharomyces YEF3 gene in this vector and demonstrated an increase in YEF3 protein levels when cells were grown in the presence of the sugar sorbitol.
McGonigal
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Pharmaceutical Products Division, Abbott Laboratories, Abbott Park, Illinois 60064-3500, USA.
Bodelle
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Schopp
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Sarthy
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D001483:Base Sequence; D005656:Fungal Proteins; D005822:Genetic Vectors; D007064:L-Iditol 2-Dehydrogenase / Q000235:genetics*; D008969:Molecular Sequence Data; D010445:Peptide Elongation Factors / Q000096:biosynthesis*; D011994:Recombinant Proteins / Q000096:biosynthesis*; D012441:Saccharomyces cerevisiae / Q000235:genetics*; D029701:Saccharomyces cerevisiae Proteins
D005656:Fungal Proteins; D010445:Peptide Elongation Factors; D011994:Recombinant Proteins; D029701:Saccharomyces cerevisiae Proteins; C111584:YEF3 protein, S cerevisiae; D007064:L-Iditol 2-Dehydrogenase