title: The telomere and telomerase: nucleic acid-protein complexes acting in a telomere homeostasis system. A review.
abstract: The tandemly repeated DNA sequence of telomeres is typically specified by the ribonucleoprotein enzyme telomerase. Telomerase copies part of its intrinsic RNA moiety to synthesize one strand of the telomeric repeat DNA Recent work, taken together with many observations over the past years, has led to the concept of a telomere homeostasis system. We have analyzed the interplay between two key physical components of this system: structural components of the telomere itself and of telomerase. Here we review some of these recent studies. The experimental method used in common in these studies was to make mutations in the template sequence of telomerase RNA, which caused various phenotypes. First, mutating specific residues in the ciliate Tetrahymena thermophila and yeast showed that these residues are required for critical aspects of the enzymatic action of telomerase. Second, certain mutated telomeric sequences caused a strong anaphase block in Tetrahymena micronuclei. Third, specific template mutations in the telomerase RNA gene led to varying degrees of telomere elongation in Tetrahymena and the yeast Kluyveromyces lactis. For some of the K. lactis mutations, the loss of length unregulated elongation was directly related to loss of binding to K. lactis Rap 1p protein. Using K. lactis carrying alterations in the telomerase RNA template, and in the gene encoding the Rap 1p protein, we found that a crucial determinant of telomere length homeostasis is the nature of the duplex DNA-Rap 1p protein complex on the very end repeat of the telomere. We propose that this complex plays a key role in regulating access of telomerase to the telomere.
authors.affiliation: Department of Microbiology and Immunology, University of California, San Francisco 94143-0414, USA. porter@itsa.ucsf.edu
authors.forename: E H
authors.initials: EH
authors.lastname: Blackburn
mesh_terms: D000818:Animals; D004247:DNA / Q000737:chemistry / Q000378:metabolism; D004261:DNA Replication; D006706:Homeostasis; D007716:Kluyveromyces / Q000235:genetics / Q000378:metabolism; D009154:Mutation; D012091:Repetitive Sequences, Nucleic Acid; D012441:Saccharomyces cerevisiae / Q000235:genetics / Q000378:metabolism; D019098:Telomerase / Q000096:biosynthesis / Q000378:metabolism*; D016615:Telomere / Q000502:physiology*; D013698:Templates, Genetic; D016808:Tetrahymena thermophila / Q000235:genetics / Q000378:metabolism
chemical_list: D004247:DNA; D019098:Telomerase