title: [Relationship between the presence of side-holes in bone marrow aspiration needle and the number of harvested bone marrow mononuclear cells].
abstract: The aim of this study was to evaluate the yield of nucleated cells in bone marrow harvested by means of Downs' Islam aspiration needles with or without side-holes. Twenty marrow donors for HLA identical sibling transplants were studied. Bone marrow nucleated cells were harvested 50 ml in the right iliac bone with a Downs' Islam aspiration needle with or without side-holes and 50 ml in the left with another kind of needle by means of 5 ml aspirations in each donor. There were no significant difference between the number of nucleated cells by means of Downs' Islam aspiration needles with and without side-holes by cross-over analysis. All nucleated cell counts were 33.06 +/- 12.93 x 10(3)/microliter (mean +/- standard deviation) by means of the needle with side-holes and 32.90 +/- 15.25 x 10(3)/microliter by means of the needle without side-holes. The Downs' Islam aspiration needle without side-holes seems to be better in bone marrow harvest from normal volunteers than that with side-holes, because the former may be stronger than the latter.
authors.affiliation: Tokyo Metropolitan Komagome Hospital, Bone marrow transplantation team.
authors.forename: S
authors.initials: S
authors.lastname: Tanikawa
authors.forename: H
authors.initials: H
authors.lastname: Sakamaki
authors.forename: S
authors.initials: S
authors.lastname: Mori
authors.forename: H
authors.initials: H
authors.lastname: Akiyama
authors.forename: H
authors.initials: H
authors.lastname: Miyamoto
authors.forename: Y
authors.initials: Y
authors.lastname: Tanaka
authors.forename: H
authors.initials: H
authors.lastname: Yoshinaga
authors.forename: R
authors.initials: R
authors.lastname: Okamoto
authors.forename: Y
authors.initials: Y
authors.lastname: Maeda
authors.forename: T
authors.initials: T
authors.lastname: Mukaiyama
authors.forename: T
authors.initials: T
authors.lastname: Sasaki
authors.forename: H
authors.initials: H
authors.lastname: Kaku
authors.forename: Y
authors.initials: Y
authors.lastname: Matsuura
authors.forename: K
authors.initials: K
authors.lastname: Hiruma
authors.forename: Y
authors.initials: Y
authors.lastname: Onozawa
authors.forename: Y
authors.initials: Y
authors.lastname: Adachi
mesh_terms: D000293:Adolescent; D000328:Adult; D001707:Biopsy, Needle / Q000295:instrumentation*; D001854:Bone Marrow Cells / Q000166:cytology*; D001856:Bone Marrow Examination / Q000295:instrumentation*; D002452:Cell Count; D004867:Equipment Design; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D009339:Needles