Dual radiolabeled liposomes: biodistribution studies and localization of focal sites of infection in rats.
Liposomes encapsulating both glutathione and deferoxamine were labeled with 99mTc-HMPAO and 111In-oxine at the same time. These dual radiolabeled liposomes were intravenously injected in rats with S. aureus infection in thigh. The target-to-background ratio (T/BG) increased from 2.9 at 2 h to 4.4 at 8 h in 99mTc images. In 111In images, T/BG of 5.5 at 8 h increased to 10.5 by 48 h. The 24-h spleen uptake of 111In- and 99mTc-liposomes was 24.14%ID and 8.91%ID. In femur, 99mTc-liposomes remained at approximately 10.5%ID, but 111In-liposomes increased from approximately 11%ID at 4 h to approximately 25.5%ID at 24 h. The simultaneous presence of 99mTc and 111In in the liposomes resulted in good early (2-8 h) as well as delayed (24-48 h) images delineating the infection site.
Awasthi
V D
VD
Department of Radiology, University of Texas Health Science Center at San Antonio, 78284, USA.
Goins
B
B
Klipper
R
R
Phillips
W T
WT
D000818:Animals; D002614:Chelating Agents / Q000493:pharmacokinetics; D003676:Deferoxamine / Q000493:pharmacokinetics; D005978:Glutathione / Q000493:pharmacokinetics; D007091:Image Processing, Computer-Assisted; D007205:Indium Radioisotopes; D007239:Infections / Q000000981:diagnostic imaging*; D007249:Inflammation / Q000000981:diagnostic imaging; D008081:Liposomes / Q000493:pharmacokinetics*; D011877:Radionuclide Imaging; D019275:Radiopharmaceuticals / Q000493:pharmacokinetics*; D051381:Rats; D017207:Rats, Sprague-Dawley; D013203:Staphylococcal Infections / Q000000981:diagnostic imaging; D019690:Technetium Tc 99m Exametazime / Q000493:pharmacokinetics; D014018:Tissue Distribution
D002614:Chelating Agents; D007205:Indium Radioisotopes; D008081:Liposomes; D019275:Radiopharmaceuticals; D019690:Technetium Tc 99m Exametazime; D005978:Glutathione; D003676:Deferoxamine