Bacterial invasion induces interleukin-7 receptor expression in colonic epithelial cell line, T84.
The intestinal epithelial layer forms the interface between the external and the internal environments of a host. Since the interleukin-7 (IL-7) and IL-7 receptor (IL-7R) signaling pathway has been shown to play an important role in the mucosal immune system, we studied the expression of IL-7R in T84, a colonic epithelial cell line, after cells were infected with several types of enteropathogenic bacteria, including Salmonella typhimurium, enteropathogenic Escherichia coli and enteroinvasive Escherichia coli. Bacterial invasion induced IL-7R expression in T84 assessed by a semi-quantitative reverse transcription-polymerase chain reaction technique and flow cytometry. The inhibition of bacterial invasion by cytochalasin D, a specific inhibitor of actin polymerization, led to a reduction in the expression of IL-7R. These data indicate that bacterial invasion into intestinal epithelial cells is likely to be an essential process in the induction of IL-7R. The communication between the epithelium and mucosal lymphocytes which is mediated via IL-7 and IL-7R may be involved in the modulation of the mucosal inflammation which occurs in bacterial infection.
Yamada
K
K
Department of Bacterial Infections, Research Institute for Microbial Diseases, Osaka University, Suita, Japan.
Shimaoka
M
M
Nagayama
K
K
Hiroi
T
T
Kiyono
H
H
Honda
T
T
D015703:Antigens, CD / Q000096:biosynthesis* / Q000276:immunology; D002460:Cell Line; D003106:Colon / Q000166:cytology / Q000276:immunology / Q000382:microbiology; D004927:Escherichia coli Infections / Q000276:immunology*; D005434:Flow Cytometry; D006801:Humans; D015851:Interleukin-7 / Q000276:immunology*; D007413:Intestinal Mucosa / Q000276:immunology* / Q000382:microbiology*; D018123:Receptors, Interleukin / Q000096:biosynthesis* / Q000276:immunology; D020395:Receptors, Interleukin-7; D012480:Salmonella Infections / Q000276:immunology*
D015703:Antigens, CD; D015851:Interleukin-7; D018123:Receptors, Interleukin; D020395:Receptors, Interleukin-7