title: Human endometrial MUC1 carries keratan sulfate: characteristic glycoforms in the luminal epithelium at receptivity.
abstract: MUC1 is a high molecular mass, highly glycosylated epithelial apical glycoprotein that has been shown to exhibit both adhesive and anti-adhesive properties. Its expression in human glandular endometrial epithelium is transcriptionally regulated with the highest levels in the mid secretory phase, the "receptive" period during which implantation occurs. We demonstrate that endometrial MUC1 carries highly sulfated lactosaminoglycan chains recognized by monoclonal antibody (Mab) 5D4, and the sialokeratan sulfate epitope recognized by Mab D9B1. These glycans are hormonally regulated in endometrium, and show increased abundance in the secretory phase, but detailed evaluation of their distribution shows important differences. The 5D4 epitope is abundant at the luminal epithelial surface until the implantation phase, when it disappears, first from patches of cells, then altogether. D9B1 binding sites are retained in the luminal epithelium at receptivity. These data show that endometrial MUC1 carries sulfated lactosaminoglycans. They identify the luminal epithelial compartment as a site of unique MUC1 glycosylation and independent regulation. Glycosylation and the negative charge associated with sialo- and sulfoglycans may be important in the regulation of embryo attachment.
authors.affiliation: Department of Obstetrics and Gynaecology, University of Manchester, United Kingdom.
authors.forename: J D
authors.initials: JD
authors.lastname: Aplin
authors.forename: N A
authors.initials: NA
authors.lastname: Hey
authors.forename: R A
authors.initials: RA
authors.lastname: Graham
mesh_terms: D000606:Amino Sugars / Q000737:chemistry / Q000276:immunology; D000911:Antibodies, Monoclonal / Q000276:immunology / Q000378:metabolism; D001665:Binding Sites / Q000276:immunology; D010064:Embryo Implantation / Q000502:physiology; D004717:Endometrium / Q000737:chemistry* / Q000166:cytology; D000939:Epitopes / Q000276:immunology; D005260:Female; D006031:Glycosylation; D006801:Humans; D007150:Immunohistochemistry; D007632:Keratan Sulfate / Q000737:chemistry* / Q000276:immunology; D018396:Mucin-1 / Q000737:chemistry*; D011134:Polysaccharides / Q000032:analysis / Q000737:chemistry / Q000276:immunology
chemical_list: D000606:Amino Sugars; D000911:Antibodies, Monoclonal; D000939:Epitopes; D018396:Mucin-1; D011134:Polysaccharides; C039448:lactosaminoglycan; D007632:Keratan Sulfate