A simple and sensitive bioassay for the detection of human interleukin-18/interferon-gamma-inducing factor using human myelomonocytic KG-1 cells.
Interleukin-18 (IL-18)/interferon-gamma-inducing factor (IGIF) is a novel cytokine which plays an important role in Th1 responses. Here we describe a simple, sensitive bioassay for human IL-18 using the human myelomonocytic cell line, KG-1, which produces IFN-gamma in response to human IL-18. IFN-gamma production induced by human IL-18 was completely blocked by an antibody against human IL-18. Human IL-18 could be measured in a concentration range from approximately 100 to 10,000 pg/ml, and intra- and inter-assay coefficient variations were both below 15%. It was possible to measure human IL-18 in human serum, cell lysate or culture supernatant by this bioassay. Thus, the human IL-18 bioassay can be expected to be useful in the investigation of the relationship between human IL-18 and various diseases or in analyzing the mechanisms of human IL-18 secretion from IL-18 producing cells.
Konishi
K
K
Fujisaki Institute, Hayashibara Biochemical Laboratories, Inc., Okayama, Japan. fujih@po.harenet.or.jp
Tanabe
F
F
Taniguchi
M
M
Yamauchi
H
H
Tanimoto
T
T
Ikeda
M
M
Orita
K
K
Kurimoto
M
M
D000818:Animals; D001681:Biological Assay; D003429:Cross Reactions; D016207:Cytokines / Q000096:biosynthesis* / Q000494:pharmacology; D006801:Humans; D007369:Interferon Inducers / Q000032:analysis* / Q000494:pharmacology; D007371:Interferon-gamma / Q000096:biosynthesis*; D020382:Interleukin-18; D051379:Mice; D012680:Sensitivity and Specificity; D014407:Tumor Cells, Cultured
D016207:Cytokines; D007369:Interferon Inducers; D020382:Interleukin-18; D007371:Interferon-gamma