Characterization and expression analysis of a Xanthomonas oryzae pv. oryzae recA.
Nucleotide sequence of Xanthomonas oryzae pv. oryzae (Xoo) DNA from pSM-A1 was determined and sequence analysis revealed an ORF with high homology to RecA proteins. Expression analysis using an anti-RecA antibody demonstrates that MMS treatment induces recA in Xanthomonas strains but not in an Escherichia coli harbouring cloned Xoo recA. This indicates the existence of a recA regulatory mechanism in Xanthomonas that is not function in E. coli. In Xoo, recA was highly induced by treatments with chemical mutagens, UV and peroxides, while superoxides, a thiol agent, a heavy metal and heat shock were not inducers. The increased amount of RecA induced by H2O2 or MMS treatments were due to increased transcription of recA. recA showed no growth phase or starvation regulation. The pattern of recA regulation in Xoo could play important roles in stress survival in the environment and during plant-microbe interactions.
Rabibhadana
S
S
Laboratory of Biotechnology, Chulabhorn Research Institute, Lak Si, Bangkok, Thailand.
Chamnongpol
S
S
Sukchawalit
R
R
Ambulos
N P
NP
Trempy
J E
JE
Mongkolsuk
S
S
D001426:Bacterial Proteins / Q000235:genetics; D015152:Blotting, Northern; D004249:DNA Damage; D015964:Gene Expression Regulation, Bacterial / Q000187:drug effects / Q000528:radiation effects; D009153:Mutagens / Q000494:pharmacology; D018384:Oxidative Stress / Q000235:genetics; D011401:Promoter Regions, Genetic; D012333:RNA, Messenger / Q000032:analysis; D011938:Rec A Recombinases / Q000032:analysis / Q000235:genetics*; D017422:Sequence Analysis, DNA; D014466:Ultraviolet Rays; D014974:Xanthomonas / Q000187:drug effects / Q000235:genetics* / Q000254:growth & development
D001426:Bacterial Proteins; D009153:Mutagens; D012333:RNA, Messenger; D011938:Rec A Recombinases