[Detection of the thermostable lipase gene (lipAH) in clinical isolates of Aeromonas hydrophila belonging to the major O serogroups O11, O16 and O34].
On the basis of DNA hybridization data and phenotypes, most pathogenic strains of Aeromonas hydrophila are grouped into hybridization group 1 (HG1). These pathogenic strains secret the theromostable lipase, and it's gene (lipAH) has been cloned and sequenced. The present study was performed to identify the pathogenic strains of A. hydrophila by the polymerase chain reaction (PCR) technique to detect the lipAH. Synthetic oligonucleotide primers (lipAH-ns-1 and -na-1) were used in the PCR. The PCR identified 80% of lipAH-positive strains, consisting of seven of the 11 O11 strains (64%), 13 of the 15 O16 strains (87%) and 25 of the 30 O34 strains (83%) in clinical isolates of A. hydrophila used in this study.
Shibata
M
M
Department of Microbiology, Kyorin University School of Health Sciences.
Morita
K
K
Watanabe
N
N
Okitsu
T
T
Yamai
S
S
Itou
K
K
Shimada
T
T
Kanamori
M
M
D016980:Aeromonas hydrophila / Q000145:classification / Q000235:genetics*; D005798:Genes, Bacterial; D006801:Humans; D008049:Lipase / Q000235:genetics*; D009693:Nucleic Acid Hybridization; D016133:Polymerase Chain Reaction / Q000379:methods; D012703:Serotyping
D008049:Lipase