A novel MT gene of rice plants is strongly expressed in the node portion of the stem.
We identified a cDNA encoding a metallothionein (MT)-like protein from a cDNA library of rice endosperm. This cDNA (ricMT) encoded an open reading frame of 80 amino acids, with two cysteine-rich domains at the amino-terminus and carboxy-terminus, respectively. The deduced amino acid sequence was homologous to those of class I MT-like proteins. Southern blot analysis revealed that the gene exists at one locus in the rice genome. Northern blot analysis with rice seedlings showed that the transcript level in shoots was elevated by the addition of metal ions, such as Cu, Zn, Cd, Fe, Pb and A1, whereas in roots it was reduced in the presence of metal ions other than copper. The expression level of ricMT in mature rice plants was extremely high in stems relative to leaf blades, leaf sheaths, endosperm and roots. In the first nodes, the yield of the transcript was 150-times higher than that in leaf blades. These results suggest that ricMT protein may play an important role in the metabolism of metal elements in the stem.
Yu
L H
LH
Institute of Molecular and Cellular Biosciences, University of Tokyo, Japan.
Umeda
M
M
Liu
J Y
JY
Zhao
N M
NM
Uchimiya
H
H
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D018744:DNA, Plant; D015870:Gene Expression; D017343:Genes, Plant; D018745:Genome, Plant; D006801:Humans; D008668:Metallothionein / Q000096:biosynthesis / Q000235:genetics*; D008670:Metals; D008969:Molecular Sequence Data; D012275:Oryza / Q000235:genetics*; D010940:Plant Proteins / Q000235:genetics*; D018547:Plant Stems / Q000378:metabolism; D018749:RNA, Plant; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D014018:Tissue Distribution
D018744:DNA, Plant; D008670:Metals; D010940:Plant Proteins; D018749:RNA, Plant; D008668:Metallothionein