Ionophoretic properties of ferutinin.
The influence of the natural terpenoid ferutinin (4-oxy-6-(4-oxybenzoyloxy) dauc-8,9-en), isolated from the plant Ferula tenuisecta, on ion permeability of biological and artificial membranes was investigated. It was shown that ferutinin, in the concentration range 1-50 microM, increases the permeability of thymocytes, mitochondria, sarcoplasmic reticulum, liposomes and bilayer lipid membranes (BLM) for Ca2+. Ferutinin establishes a transmembrane potential in BLM equal to the Nernst's potential. The permeability ratio for Na+/Ca2+ is 0.41. The dependence of BLM conductivity on ferutinin concentration is linear. The stoichiometry of the ferutinin:Ca2+ complex is 2, assuming the formation of a structure with participation of two terpenoid molecules and one Ca2+ ion.
Zamaraeva
M V
MV
Department of Biophysics, Tashkent State University, Uzbekistan. root@ibc.tashkent.su
Hagelgans
A I
AI
Abramov
A Y
AY
Ternovsky
V I
VI
Merzlyak
P G
PG
Tashmukhamedov
B A
BA
Saidkhodzjaev
A I
AI
D000818:Animals; D001565:Benzoates / Q000494:pharmacology*; D001643:Bridged Bicyclo Compounds; D002118:Calcium / Q000378:metabolism; D002462:Cell Membrane / Q000187:drug effects / Q000378:metabolism; D002463:Cell Membrane Permeability; D003508:Cycloheptanes; D007425:Intracellular Membranes / Q000187:drug effects / Q000378:metabolism; D007476:Ionophores / Q000494:pharmacology*; D008081:Liposomes; D008928:Mitochondria / Q000187:drug effects / Q000378:metabolism; D015394:Molecular Structure; D010539:Permeability; D010944:Plants / Q000737:chemistry; D051381:Rats; D012519:Sarcoplasmic Reticulum / Q000187:drug effects / Q000378:metabolism; D012717:Sesquiterpenes; D013950:Thymus Gland / Q000166:cytology / Q000187:drug effects / Q000378:metabolism
D001565:Benzoates; D001643:Bridged Bicyclo Compounds; D003508:Cycloheptanes; D007476:Ionophores; D008081:Liposomes; D012717:Sesquiterpenes; C111068:4-oxy-6-(4-oxybezoyloxy)dauc-8,9-en; D002118:Calcium