Kinetics of phenylalanine transport at the human blood-brain barrier investigated in vivo.
In vivo proton magnetic resonance spectroscopy was used to investigate intracerebral phenylalanine (Phe) concentrations in nine patients with classical phenylketonuria (PKU). The study included serial examinations (n = 31; plasma Phe levels: 0.47-2.24 mmol/l) of patients either receiving a Phe-restricted diet (200 mg Phe per day; four patients) or a diet rich in Phe (1000 mg Phe per day; three patients). No spectrum showed metabolic abnormalities besides elevated Phe. Difference spectroscopy yielded intracerebral Phe concentrations between 0.20 and 0.76 mmol/l. Regional variations between parieto-occipital periventricular brain, frontal brain, and cerebellum were not statistically significant. Data could be fitted assuming saturable Phe transport into the brain, based on a symmetric Michaelis-Menten model (characterized by an apparent Michaelis transport constant, K(t,app), and a maximum transport velocity, Tmax) and constant Phe consumption in the brain cells (described by a velocity Vmax). Non-linear least-squares fitting of the combined data from all patients yielded K(t,app) = 0.16 +/- 0.11 mmol/l and (Tmax / Vmax) = 9.0 +/- 4.1. Carrier saturation and competitive inhibition of the influx of other large neutral amino acids can be expected at plasma Phe levels usually found in PKU patients.
Möller
H E
HE
Center for In Vivo Microscopy, Duke University Medical Center, Durham, NC 27710, USA. hem@orion.mc.duke.edu
Weglage
J
J
Wiedermann
D
D
Vermathen
P
P
Bick
U
U
Ullrich
K
K
D000293:Adolescent; D000328:Adult; D001812:Blood-Brain Barrier / Q000502:physiology*; D001923:Brain Chemistry / Q000502:physiology; D002648:Child; D005260:Female; D006801:Humans; D007700:Kinetics; D009682:Magnetic Resonance Spectroscopy; D008297:Male; D010649:Phenylalanine / Q000493:pharmacokinetics*; D010661:Phenylketonurias / Q000378:metabolism*
D010649:Phenylalanine