title: A mutation in Paramecium tetraurelia reveals functional and structural features of developmentally excised DNA elements.
abstract: The excision of internal eliminated sequences (IESs) from the germline micronuclear DNA occurs during the differentiation of a new macronuclear genome in ciliated protozoa. In Paramecium, IESs are generally short (28-882 bp), AT rich DNA elements that show few conserved sequence features with the exception of an inverted-terminal-repeat consensus sequence that has similarity to the ends of mariner/Tcl transposons (KLOBUTCHER and HERRICK 1995). We have isolated and analyzed a mutant cell line that cannot excise a 370-bp IESs (IES2591) from the coding region of the 51A variable surface protein gene. A single micronuclear C to T transition within the consensus sequence prevents excision. The inability to excise IES259 I has revealed a 28-bp IES inside the larger IES, suggesting that reiterative integration of these elements can occur. Together, the consensus sequence mutation and the evidence for reiterative integration support the theory that Paramecium IESs evolved from transposable elements. Unlike a previously studied Paramecium IES, the presence of this IES in the macronucleus does not completely inhibit excision of its Mild-type micronuclear copy through multiple sexual generations.
authors.affiliation: Department of Biochemistry, Purdue University, West Lafayette, Indiana 47907-1153, USA.
authors.forename: K M
authors.initials: KM
authors.lastname: Mayer
authors.forename: K
authors.initials: K
authors.lastname: Mikami
authors.forename: J D
authors.initials: JD
authors.lastname: Forney
mesh_terms: D000818:Animals; D001483:Base Sequence; D002460:Cell Line; D004251:DNA Transposable Elements; D016054:DNA, Protozoan / Q000235:genetics*; D017125:Genes, Protozoan / Q000235:genetics*; D008969:Molecular Sequence Data; D016805:Paramecium tetraurelia / Q000235:genetics*; D010641:Phenotype; D016415:Sequence Alignment; D017384:Sequence Deletion
chemical_list: D004251:DNA Transposable Elements; D016054:DNA, Protozoan