Synthesis of a new neoglycolipid (AgH-1) and its effect upon the properties of dipalmitoylphosphatidylcholine: cholesterol liposomes.
A new neoglycolipid (AgH-1) bearing carbohydrate units that mimics the antigenic determinant of the O-blood group was synthesized and the effect of its incorporation in dipalmitoylphosphatidylcholine (DPPC): cholesterol liposomes was evaluated. The results obtained show that AgH-1 is readily incorporated into DPPC:cholesterol liposomes. The conditions leading to the optimal incorporation are the result of a compromise between incorporation efficiency and incorporation extent. The presence of AgH-1 produces liposomes of smaller size, with only small changes in the properties of the bilayer. However, the data obtained employing diphenylhexatriene and laurodan as fluorescence probes and merocyanine 540 as optical probe suggest that AgH-1 incorporation leads to a small rigidization of the liposomes at temperatures lower than ca. 42 degrees C.
Lissi
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EA
Faculty of Chemistry and Biology, University of Santiago de Chile, Casilla 40-Correo 33, Santiago, Chile.
Abuin
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EB
Lanio
M E
ME
Alvarez
C
C
Luzardo
M C
MC
Figueroa
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S
Verez
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V
D001789:Blood Group Antigens / Q000276:immunology; D002240:Carbohydrate Sequence; D002784:Cholesterol / Q000378:metabolism; D004161:Diphenylhexatriene / Q000378:metabolism; D004910:Erythrocyte Membrane / Q000737:chemistry; D005454:Fluorescence Polarization; D005456:Fluorescent Dyes / Q000378:metabolism; D006017:Glycolipids / Q000737:chemistry* / Q000494:pharmacology*; D007700:Kinetics; D008051:Lipid Bilayers / Q000737:chemistry; D008081:Liposomes / Q000737:chemistry* / Q000378:metabolism; D008969:Molecular Sequence Data; D009281:Naphthalenes / Q000378:metabolism; D010316:Particle Size; D010713:Phosphatidylcholines / Q000378:metabolism; D011744:Pyrimidinones / Q000378:metabolism; D013696:Temperature
D001789:Blood Group Antigens; D005456:Fluorescent Dyes; D006017:Glycolipids; D008051:Lipid Bilayers; D008081:Liposomes; D009281:Naphthalenes; D010713:Phosphatidylcholines; D011744:Pyrimidinones; C093831:laurodan; D004161:Diphenylhexatriene; C003954:merocyanine dye; D002784:Cholesterol