title: Lipopolysaccharide enhances synthesis of brain-derived neurotrophic factor in cultured rat microglia.
abstract: Expression of neurotrophins in pure microglia cultured from embryonic rat brain and the effects of lipopolysaccharide (LPS) on the expression were investigated. In untreated cultures, nerve growth factor (NGF), brain-derived neurotrophic factor (BDNF), and neurotrophin (NT)-4/5 mRNAs were detected by use of reverse transcriptase-polymerase chain reaction but NT-3 mRNA was not. LPS stimulation caused a marked increase in BDNF mRNA expression in addition to a slight increment of the NT-4/5 mRNA level; however, the NGF mRNA level was not affected. LPS also increased BDNF-like immunoreactivity in cultured microglia, an action consistent with an elevation of BDNF mRNA. These results demonstrate that LPS stimulates synthesis of BDNF and probably NT-4/5, specific ligands for tyrosine kinase receptor TrkB, suggesting that activated microglia, which appear in the damaged brain, participate in neuronal regeneration via production of such neurotrophins.
authors.affiliation: Laboratory of Molecular Biology, Gifu Pharmaceutical University, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Miwa
authors.forename: S
authors.initials: S
authors.lastname: Furukawa
authors.forename: K
authors.initials: K
authors.lastname: Nakajima
authors.forename: Y
authors.initials: Y
authors.lastname: Furukawa
authors.forename: S
authors.initials: S
authors.lastname: Kohsaka
mesh_terms: D000818:Animals; D019208:Brain-Derived Neurotrophic Factor / Q000096:biosynthesis*; D002478:Cells, Cultured; D007120:Immunochemistry; D008070:Lipopolysaccharides / Q000494:pharmacology*; D017628:Microglia / Q000187:drug effects* / Q000378:metabolism; D009414:Nerve Growth Factors / Q000096:biosynthesis*; D020933:Neurotrophin 3; D016133:Polymerase Chain Reaction / Q000379:methods; D012333:RNA, Messenger / Q000096:biosynthesis; D012194:RNA-Directed DNA Polymerase; D051381:Rats
chemical_list: D019208:Brain-Derived Neurotrophic Factor; D008070:Lipopolysaccharides; D009414:Nerve Growth Factors; D020933:Neurotrophin 3; D012333:RNA, Messenger; D012194:RNA-Directed DNA Polymerase