title: Extremely low frequency magnetic fields promote neurite varicosity formation and cell excitability in cultured rat chromaffin cells.
abstract: We report the use of an extremely low frequency magnetic field (ELF-MF, 60 Hz, 10 Grms) to stimulate cultured chromaffin cells to determine the possible changes that may occur in their electrical properties. The results showed that ELF-MF not only facilitated neurite outgrowth, but also formation of neurite varicosity with high concentration of catecholamines. This report also shows for the first time that a greater number of cultured chromaffin cells differentiated by ELF-MF have spontaneous extracellular electrical activity and that their firing frequency is higher than that seen in non-stimulated cells.
authors.affiliation: Depto. de Fisiología, Universidad Nacional Autónoma de México, México, D.F.
authors.forename: L
authors.initials: L
authors.lastname: Zhang
authors.forename: L
authors.initials: L
authors.lastname: Zhou
authors.forename: A
authors.initials: A
authors.lastname: Vega-González
authors.forename: D
authors.initials: D
authors.lastname: Mendoza
authors.forename: R
authors.initials: R
authors.lastname: Drucker-Colín
mesh_terms: D000200:Action Potentials; D000313:Adrenal Medulla / Q000166:cytology; D000818:Animals; D002395:Catecholamines / Q000032:analysis; D002454:Cell Differentiation; D002455:Cell Division; D002478:Cells, Cultured; D019439:Chromaffin Cells / Q000473:pathology*; D004574:Electromagnetic Fields; D007150:Immunohistochemistry; D009414:Nerve Growth Factors; D016501:Neurites / Q000737:chemistry / Q000473:pathology*; D018408:Patch-Clamp Techniques; D051381:Rats; D017208:Rats, Wistar; D014446:Tyrosine 3-Monooxygenase / Q000032:analysis
chemical_list: D002395:Catecholamines; D009414:Nerve Growth Factors; D014446:Tyrosine 3-Monooxygenase