Antigen-primed T cells from B cell-deficient JHD mice fail to provide B cell help.
B cells are effective in presenting Ag to primed T cells, but a specific role for B cells in priming naive T cells has not been clearly established. In this report we demonstrate that Ag administration to B cell-deficient JHD mice primes T cells for Ag-specific proliferative responses, but such primed T cells fail to provide help for isotype switching and IgG production in B cells. Although T cells primed in the absence of B cells could proliferate in response to Ag presented by B cells and could induce Ag-specific IgM production, such T cells failed to produce high levels of IL-4 as are normally induced in T cells by Ag-presenting B cells. These findings suggest that while B cells are not absolutely required for T cell priming, they provide signals to T cells that are not replicated by other APCs and that influence the subsequent ability of T cells to interact with B cells.
Macaulay
A E
AE
Department of Pediatrics, Stanford University, CA 94305, USA.
DeKruyff
R H
RH
Umetsu
D T
DT
D000818:Animals; D000917:Antibody Formation / Q000235:genetics; D000938:Antigen-Presenting Cells / Q000276:immunology; D000941:Antigens / Q000276:immunology*; D001402:B-Lymphocytes / Q000276:immunology* / Q000473:pathology; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology; D002469:Cell Separation; D016207:Cytokines / Q000096:biosynthesis; D007153:Immunologic Deficiency Syndromes / Q000235:genetics* / Q000276:immunology / Q000473:pathology; D015847:Interleukin-4 / Q000172:deficiency / Q000378:metabolism; D008198:Lymph Nodes / Q000276:immunology / Q000378:metabolism / Q000473:pathology; D008213:Lymphocyte Activation / Q000235:genetics; D008211:Lymphocyte Cooperation / Q000235:genetics*; D051379:Mice; D008807:Mice, Inbred BALB C; D008822:Mice, Transgenic; D016176:T-Lymphocyte Subsets / Q000276:immunology* / Q000378:metabolism; D006377:T-Lymphocytes, Helper-Inducer / Q000276:immunology*
D000941:Antigens; D016207:Cytokines; D015847:Interleukin-4