FISH mapping of seven cDNA sequences in the pig.
Fluorescence in situ hybridization (FISH) technique was applied to localize seven clones derived from a porcine (SSC) intestinal directionally cloned cDNA library. The size of the clones ranged from 1.1 to 1.3 kb. Three of the clones corresponded to histidyl-tRNA synthetase (HARS), immunoglobulin alpha (IGA) and lysozyme (LYZ) and mapped to SSC2q28-q29, 7q2.6 and 5p11 respectively. The available human-pig comparative painting data and sequence homology comparisons assisted in a tentative identification of the other three clones as glutathione-S-transferase (GST), glutathione-S-transferase mu (GSTM1) and immunoglobulin lambda gene cluster (IGL@). These clones mapped to SSC14q21, 5q2.4 and 14q22-q23 respectively. The remaining clone representing an EST mapped to 1p24-p25. These localizations contribute to the transcript map in pig and are significant as comparative markers. Difficulties associated with the mapping of small sequences using FISH are discussed.
Chaudhary
R
R
Department of Animal Breeding and Genetics, Swedish University of Agricultural Sciences, Uppsala.
Winterø
A K
AK
Fredholm
M
M
Chowdhary
B P
BP
D000818:Animals; D002874:Chromosome Mapping / Q000379:methods*; D018076:DNA, Complementary / Q000235:genetics*; D015870:Gene Expression; D005803:Genes, Immunoglobulin / Q000235:genetics; D005982:Glutathione Transferase / Q000235:genetics; D006643:Histidine-tRNA Ligase / Q000235:genetics; D017404:In Situ Hybridization, Fluorescence / Q000379:methods*; D005810:Multigene Family; D009113:Muramidase / Q000235:genetics; D012689:Sequence Homology, Nucleic Acid; D013552:Swine / Q000235:genetics*
D018076:DNA, Complementary; D005982:Glutathione Transferase; D009113:Muramidase; D006643:Histidine-tRNA Ligase