HLA class I and class II allele distribution in the Bubi population from the island of Bioko (Equatorial Guinea).
We determined the HLA frequency distribution in a sample of 100 Bubi individuals born on the island of Bioko (Equatorial Guinea). HLA-A, -B and -C typing was performed by serology and PCR-SSP. DRB1/3/4/5, DQB1 and DQA1 alleles were determined by PCR-SSOP. The HLA allele distribution of this population group resembles those found in other Bantu-speaking groups; however, the higher frequency of A30, A32, B44, DRB1*1301 in the Bubi with respect to other Bantu groups and the absence of DR4 deserve special mention. The cloning and sequencing of class I and II genes in this population allowed the description of five new allelic variants: B*4407, Cw*0706, Cw*1801, Cw*1802 and DQB1*0612 and five confirmatory sequences: B*3910, B*5703, B*8101, Cw*1203 and Cw*1701. The following new HLA-C,B haplotypes have been found in Bubi: Cw*08-B*57, Cw*18-B*57, Cw*0302-B*53, Cw*07-B*53 and Cw*1601-B*63. The most frequent seven-locus haplotype is: A*30-Cw*17-B*42-DRB1*1102-DRB3*0202-DQA1*05-DQB1 *0301. In terms of genetic distance, the Bubi are closer to other Bantu groups than to West African populations.
de Pablo
R
R
Servicio de Inmunología, Clínica Puerta de Hierro, Madrid, Spain. rpablo@inmuno.cph.es
García-Pacheco
J M
JM
Vilches
C
C
Moreno
M E
ME
Sanz
L
L
Rementería
M C
MC
Puente
S
S
Kreisler
M
M
D000483:Alleles; D016703:Equatorial Guinea; D005787:Gene Frequency; D005805:Genes, MHC Class I; D005802:Genes, MHC Class II; D015235:HLA-B Antigens / Q000235:genetics; D015236:HLA-C Antigens / Q000235:genetics; D006801:Humans; D016133:Polymerase Chain Reaction; D011153:Population
D015235:HLA-B Antigens; D015236:HLA-C Antigens