title: Phenotypic and functional characterization of mast cells derived from renal tumor tissues.
abstract: Mast cells (MCs) originate from multipotent hematopoietic progenitor cells. However, MCs in various organs are heterogenous in terms of mediator or receptor expression and response to diverse stimuli. We characterized the phenotype and functional properties of human renal mast cells (HRMCs). Tissue was obtained from 17 patients suffering from renal tumors (transitional cell carcinoma, n = 4; renal cell carcinoma, n = 13). HRMCs were isolated by collagenase digestion. Double staining with toluidine blue and immunofluorescence using monoclonal antibodies (mAbs) revealed expression of stem cell factor (SCF)-receptor (c-kit/CD117), CD9, CD29, CD33, CD43, CD44, CD54, and CD63 on HRMCs. In contrast, HRMCs were not recognized by mAbs to CD2, CD3, CD4, CD11b, CD14, CD15, CD16, CDw17, CD19, or CD23. HRMCs were also negative for CD116 (granulocyte-macrophage colony-stimulating factor [GM-CSF] receptor alpha), CD123 (interleukin [IL]-3Ralpha), CD121a (IL-1R type I), CD122 (IL-2Rbeta), and CD127 (IL-7R) and were also found to lack C5aR (CD88). Ligand-induced activation of HRMCs through immunoglobulin (Ig)E-R or SCF-R (c-kit) resulted in histamine secretion (control: <10%; alphaIgE, 1 microg/mL: 50.12 +/-5.18%; rhSCF, 100 ng/mL: 29.24 +/- 22.39), whereas recombinant C5a, erythropoietin (EPO), IL-1 through 10, and GM-CSF exerted no effects. As determined by in situ staining, HRMCs contained tryptase, but only low or undetectable amounts of chymase. Electron microscopy confirmed the presence of MCs in renal tissues and revealed a scroll-rich granule population in HRMCs. Together, HRMCs are tryptase+, C5aR- mast cells exhibiting phenotypic and functional properties similar to those of lung MCs.
authors.affiliation: Department of Internal Medicine I, Division of Hematology and Hemostaseology, The University of Vienna, Austria.
authors.forename: W J
authors.initials: WJ
authors.lastname: Beil
authors.forename: W
authors.initials: W
authors.lastname: Füreder
authors.forename: H
authors.initials: H
authors.lastname: Wiener
authors.forename: K
authors.initials: K
authors.lastname: Grossschmidt
authors.forename: U
authors.initials: U
authors.lastname: Maier
authors.forename: A
authors.initials: A
authors.lastname: Schedle
authors.forename: H C
authors.initials: HC
authors.lastname: Bankl
authors.forename: K
authors.initials: K
authors.lastname: Lechner
authors.forename: P
authors.initials: P
authors.lastname: Valent
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000911:Antibodies, Monoclonal; D015703:Antigens, CD / Q000032:analysis*; D002292:Carcinoma, Renal Cell / Q000276:immunology* / Q000473:pathology; D002295:Carcinoma, Transitional Cell / Q000276:immunology* / Q000473:pathology; D053818:Chymases; D005260:Female; D006632:Histamine / Q000032:analysis; D006636:Histamine Release; D006801:Humans; D016130:Immunophenotyping; D007668:Kidney / Q000166:cytology / Q000276:immunology / Q000473:pathology; D007680:Kidney Neoplasms / Q000276:immunology* / Q000473:pathology; D008297:Male; D008407:Mast Cells / Q000276:immunology* / Q000473:pathology / Q000648:ultrastructure; D008875:Middle Aged; D012016:Reference Values; D012697:Serine Endopeptidases / Q000032:analysis; D053802:Tryptases
chemical_list: D000911:Antibodies, Monoclonal; D015703:Antigens, CD; D006632:Histamine; D012697:Serine Endopeptidases; C083294:chymase 2; D053818:Chymases; D053802:Tryptases