The pathogenic neisseriae contain an inactive rpoN gene and do not utilize the pilE sigma54 promoter.
The sigma54 promoter (P3) upstream of the pilE gene in Neisseria gonorrhoeae was shown to be non-functional by transcriptional analysis of a PpilE::lacZ fusion containing only P3. A region on the chromosome of N. gonorrhoeae strain MS11-A was identified that potentially encodes a protein with a significant similarity to the Escherichia coli RpoN protein. However, this region (designated RLS for rpoN-like sequence) does not contain a single open reading frame (ORF) capable of encoding a functional RpoN protein. It appears that RLS may have arisen from an ancestral rpoN homologue that underwent a deletion removing the sequence encoding the essential helix-turn-helix (HTH) motif, and changing the subsequent reading frame. An RLS has been identified in several strains of N. gonorrhoeae and N. meningitidis. A 90-kDa gonococcal protein has previously been shown to react with a monoclonal antibody raised against the RpoN from Salmonella typhimurium. However, mutagenesis and Western blot analysis confirmed that the gene encoding this protein is not contained within RLS.
Laskos
L
L
Department of Microbiology, Monash University, Clayton, VIC 3168, Australia.
Dillard
J P
JP
Seifert
H S
HS
Fyfe
J A
JA
Davies
J K
JK
D000595:Amino Acid Sequence; D000911:Antibodies, Monoclonal / Q000276:immunology; D001426:Bacterial Proteins / Q000737:chemistry / Q000235:genetics* / Q000276:immunology; D003001:Cloning, Molecular; D004268:DNA-Binding Proteins; D012321:DNA-Directed RNA Polymerases / Q000235:genetics* / Q000276:immunology; D029968:Escherichia coli Proteins; D036781:Fimbriae Proteins; D005798:Genes, Bacterial; D005816:Genetic Complementation Test; D008562:Membrane Glycoproteins / Q000235:genetics*; D008969:Molecular Sequence Data; D009344:Neisseria gonorrhoeae / Q000235:genetics*; D009345:Neisseria meningitidis / Q000235:genetics*; D009693:Nucleic Acid Hybridization; D016366:Open Reading Frames; D018933:Pili, Sex / Q000235:genetics / Q000378:metabolism; D011401:Promoter Regions, Genetic; D051877:RNA Polymerase Sigma 54; D012808:Sigma Factor / Q000235:genetics* / Q000276:immunology; D014158:Transcription, Genetic
D000911:Antibodies, Monoclonal; D001426:Bacterial Proteins; D004268:DNA-Binding Proteins; D029968:Escherichia coli Proteins; D008562:Membrane Glycoproteins; C111030:RLS protein, Neisseria gonorrhoeae; D012808:Sigma Factor; C074063:pilE protein, Neisseria gonorrhoeae; C497751:rpoN protein, E coli; D036781:Fimbriae Proteins; D012321:DNA-Directed RNA Polymerases; D051877:RNA Polymerase Sigma 54