Induction and prevention of squamous transformation of bronchial epithelial cells by phenolic compounds.
1. The exposure of cultured bovine bronchial epithelial cells to 2-naphthol and catechol (10-100 microM) induced a squamous phenotype and inhibited cell proliferation. Phenol and cresol did not have these effects at up to 100 microM. 2. These effects occurred below cytotoxic levels and cell proliferation resumed subsequent to removal of the exposures. 3. Induction of the squamous phenotype was antagonized by retinoic acid, a known morphogenic hormone for several airway epithelial cell culture model systems. 4. Purified recombinant bovine phenol sulphotransferase was used to ablate the antiproliferative and squamous inductive effects of catechol on the cultured airway epithelial cells.
Palmatier
R N
RN
Department of Biochemistry, Alma College, MI 48801, USA.
Henry
T
T
Rennard
S I
SI
Beckmann
J D
JD
D000818:Animals; D015239:Arylsulfotransferase / Q000378:metabolism; D001980:Bronchi / Q000166:cytology* / Q000187:drug effects; D002396:Catechols / Q000378:metabolism; D002417:Cattle; D002469:Cell Separation; D002471:Cell Transformation, Neoplastic / Q000187:drug effects*; D003470:Culture Media; D004847:Epithelial Cells / Q000187:drug effects*; D009284:Naphthols / Q000378:metabolism; D010636:Phenols / Q000494:pharmacology*; D015262:Xenobiotics / Q000378:metabolism / Q000633:toxicity
D002396:Catechols; D003470:Culture Media; D009284:Naphthols; D010636:Phenols; D015262:Xenobiotics; D015239:Arylsulfotransferase