The human homolog of rat Jagged1 expressed by marrow stroma inhibits differentiation of 32D cells through interaction with Notch1.
A cDNA clone encoding the human homolog of rat Jagged1 was isolated from normal human marrow. Analyses of human stromal cell lines indicate that this gene, designated hJagged1, is expressed by marrow stromal cells typified by the cell line HS-27a, which supports the long-term maintenance of hematopoietic progenitor cells. G-CSF-induced differentiation of 32D cells expressing Notch1 was inhibited by coculturing with HS-27a. A peptide corresponding to the Delta/Serrate/LAG-2 domain of hJagged1 and supernatants from COS cells expressing a soluble form of the extracellular portion of hJagged1 were able to mimic this effect. These observations suggest that hJagged1 may function as a ligand for Notch1 and play a role in mediating cell fate decisions during hematopoiesis.
Li
L
L
Stower Institute for Medical Research, Department of Molecular Biotechnology, University of Washington, Seattle 98195, USA.
Milner
L A
LA
Deng
Y
Y
Iwata
M
M
Banta
A
A
Graf
L
L
Marcovina
S
S
Friedman
C
C
Trask
B J
BJ
Hood
L
L
Torok-Storb
B
B
D000328:Adult; D000595:Amino Acid Sequence; D000818:Animals; D001854:Bone Marrow Cells / Q000166:cytology* / Q000378:metabolism*; D019556:COS Cells / Q000378:metabolism; D002135:Calcium-Binding Proteins; D002454:Cell Differentiation / Q000502:physiology; D002460:Cell Line; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism; D015870:Gene Expression; D006412:Hematopoietic Stem Cells / Q000166:cytology / Q000378:metabolism; D006801:Humans; D036341:Intercellular Signaling Peptides and Proteins; D000072100:Jagged-1 Protein; D008565:Membrane Proteins / Q000096:biosynthesis / Q000235:genetics / Q000502:physiology*; D008969:Molecular Sequence Data; D017434:Protein Structure, Tertiary; D051381:Rats; D051881:Receptor, Notch1; D011956:Receptors, Cell Surface; D017386:Sequence Homology, Amino Acid; D000072099:Serrate-Jagged Proteins; D017154:Stromal Cells / Q000166:cytology / Q000378:metabolism; D014157:Transcription Factors
D002135:Calcium-Binding Proteins; D018076:DNA, Complementary; D036341:Intercellular Signaling Peptides and Proteins; C000606273:JAG1 protein, human; C000606275:Jag1 protein, rat; D000072100:Jagged-1 Protein; D008565:Membrane Proteins; C497761:NOTCH1 protein, human; C497763:Notch1 protein, rat; D051881:Receptor, Notch1; D011956:Receptors, Cell Surface; D000072099:Serrate-Jagged Proteins; D014157:Transcription Factors