[RNA typing of granulocyte NA antigens by means of PCR-RFLP analysis].
An RNA-based method was developed for genotyping of the granulocyte-specific alloantigens NA1 and NA2, mRNA was isolated from neutrophils and reversely transcribed into cDNA. To avoid coamplification of Fc-gamma receptor III-2 in the polymerase chain reaction (PCR), a Fc-gamma receptor III-1 sequence-specific primer was employed. Restriction analysis using Taq I led to NA-specific restriction fragment length polymorphism (RFLP) of PCR-amplified cDNA. The results of the PCR-RFLP typing were in good concordance with serological phenotyping by granulocyte immunofluorescence test and the antigen capture assay MAIGA.
Stein
E L
EL
Institut für Klinische Immunologie und Transfusionsmedizin, Justus-Liebig-Universität Giessen, Deutschland.
Bux
J
J
Santoso
S
S
Kalb
R
R
Mueller-Eckhardt
C
C
D006098:Granulocytes / Q000276:immunology*; D006801:Humans; D007519:Isoantigens / Q000096:biosynthesis / Q000235:genetics*; D009504:Neutrophils / Q000276:immunology; D016133:Polymerase Chain Reaction / Q000379:methods; D012150:Polymorphism, Restriction Fragment Length; D012333:RNA, Messenger / Q000096:biosynthesis
D007519:Isoantigens; D012333:RNA, Messenger
RNA-Typisierung granulozytärer NA-Antigene mit Hilfe der PCR-RFLP-Analyse.