title: Cytogenetic evaluation of occupational exposure to external gamma-rays and internal 241Am contamination.
abstract: Chromosome analyses were performed on peripheral lymphocytes from seven radiation workers exposed to external gamma-radiation within maximum annual permissible dose limits during 11 to 22 years of employment. Five years prior to blood sampling, six workers had additionally incorporated the alpha-emitting radionuclide 241Am which contributed between five to 25% of the total accumulated whole body dose in five workers and about 70% in one worker. For the radiation workers as a group, both the mean frequencies of symmetrical translocations measured by fluorescence in situ hybridisation (FISH)/chromosome painting and dicentrics + ring chromosomes scored in first division metaphases of conventional preparations were significantly elevated compared with respective controls. The mean biodosimetry estimate for the group was 270 mSv when based on stable translocations which compares well with the mean dose of 247 mSv based on official dosimetry records. The lower mean dose estimate of 160 mSv based on unstable dicentrics is compatible with the well-known loss of dicentrics from the circulating blood with time after exposure.
authors.affiliation: GSF-National Research Center for Environment, Oberschleissheim, Germany.
authors.forename: M
authors.initials: M
authors.lastname: Bauchinger
authors.forename: E
authors.initials: E
authors.lastname: Schmid
authors.forename: H
authors.initials: H
authors.lastname: Braselmann
mesh_terms: D000328:Adult; D000576:Americium / Q000009:adverse effects*; D002869:Chromosome Aberrations; D004307:Dose-Response Relationship, Radiation; D005720:Gamma Rays / Q000009:adverse effects*; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D008297:Male; D008875:Middle Aged; D009688:Nuclear Reactors; D016273:Occupational Exposure; D011829:Radiation Dosage; D014178:Translocation, Genetic; D014916:Whole-Body Irradiation
chemical_list: D000576:Americium