In vivo expression of IL-4, IL-5, IL-13 and IFN-gamma mRNAs in peripheral blood mononuclear cells and effect of cyclosporin A in a patient with Kimura's disease.
Semiquantitative reverse transcription-polymerase chain reaction was used to analyse the expression of cytokine mRNAs in freshly isolated peripheral blood mononuclear cells obtained from a patient with Kimura's disease. The patient was treated with cyclosporin A (CsA) after incomplete tumour resection and irradiation of lesions. Levels of interleukin (IL)-4, IL-5 and IL-13 mRNA were elevated and the level of interferon (IFN)-gamma mRNA was within normal limits before treatment. The levels of IL-4, IL-5 and IL-13 mRNA, the number of eosinophils, and the serum level of IgE decreased markedly after surgery and radiation therapy. CsA treatment suppressed these values in a dose-dependent manner, but had a minimal effect on the level of IFN-gamma mRNA. The number of peripheral eosinophils decreased in association with decreases in the levels of IL-4, IL-5 and IL-13 mRNAs during CsA therapy; the serum level of IgE remained low during therapy and did not fluctuate in association with changes in cytokine levels. These results suggest the Th2 cytokines play a part in the development of Kimura's disease and that CsA suppresses the activity of this disease.
Katagiri
K
K
Department of Dermatology, Oita Medical University, Japan.
Itami
S
S
Hatano
Y
Y
Yamaguchi
T
T
Takayasu
S
S
D000368:Aged; D000796:Angiolymphoid Hyperplasia with Eosinophilia / Q000097:blood* / Q000188:drug therapy / Q000276:immunology; D016572:Cyclosporine / Q000627:therapeutic use*; D004804:Eosinophils / Q000473:pathology; D015870:Gene Expression; D006801:Humans; D007073:Immunoglobulin E / Q000097:blood; D007166:Immunosuppressive Agents / Q000627:therapeutic use*; D007371:Interferon-gamma / Q000097:blood* / Q000235:genetics; D007378:Interleukins / Q000097:blood* / Q000235:genetics; D007958:Leukocyte Count / Q000187:drug effects; D007963:Leukocytes, Mononuclear / Q000276:immunology*; D008297:Male; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000235:genetics
D007166:Immunosuppressive Agents; D007378:Interleukins; D012333:RNA, Messenger; D007073:Immunoglobulin E; D007371:Interferon-gamma; D016572:Cyclosporine