title: Serological response of patients infected with Salmonella typhi.
abstract: OBJECTIVE
  To evaluate a rapid immunoblotting procedure for providing evidence of infection with Salmonella typhi using 73 sera from patients infected with S typhi.
  
  
  METHODS
  A sodium dodecyl sulphatepolyacrylamide gel electrophoresis (SDS-PAGE)/immunoblotting procedure using lipopolysaccharide (LPS, O = 9,12) and flagellar (H = d) antigens was used.
  
  
  RESULTS
  Seventy two of 73 sera contained antibodies to LPS, 40 sera also contained antibodies to H = d flagellar antigens. Analysis of acute and convalescent sera showed that only 62% of patients produced antibodies to flagellar antigens.
  
  
  CONCLUSIONS
  The SDS-PAGE/immunoblotting procedure provided a rapid method for providing serological evidence of infection with S typhi.
authors.affiliation: Laboratory of Enteric Pathogens, Central Public Health Laboratory, London, UK.
authors.forename: H
authors.initials: H
authors.lastname: Chart
authors.forename: B
authors.initials: B
authors.lastname: Rowe
authors.forename: J S
authors.initials: JS
authors.lastname: Cheesbrough
mesh_terms: D000208:Acute Disease; D000293:Adolescent; D000328:Adult; D000907:Antibodies, Bacterial / Q000097:blood*; D000942:Antigens, Bacterial / Q000276:immunology; D015415:Biomarkers / Q000097:blood; D004591:Electrophoresis, Polyacrylamide Gel; D005069:Evaluation Studies as Topic; D005260:Female; D005407:Flagella / Q000276:immunology; D006801:Humans; D015151:Immunoblotting; D008070:Lipopolysaccharides / Q000276:immunology; D008297:Male; D008875:Middle Aged; D012477:Salmonella enteritidis / Q000276:immunology; D012485:Salmonella typhi / Q000276:immunology*; D014435:Typhoid Fever / Q000175:diagnosis* / Q000276:immunology
chemical_list: D000907:Antibodies, Bacterial; D000942:Antigens, Bacterial; D015415:Biomarkers; D008070:Lipopolysaccharides