Characterization of nociceptin-stimulated in situ [35S]GTPgammaS binding in comparison with opioid agonist-stimulated ones in brain regions of the mice.
We studied the characterization of receptor-mediated G protein activity by nociceptin throughout brain regions, using in situ GTPgammaS binding autoradiography. Nociceptin-stimulated GTPgammaS binding was markedly observed in amygdala, hippocampal pyramidal cell layers, temporal and entorhinal cortex, infralimbic organ, anterior olfactory nucleus, and rostral part of thalamus. These nociceptin-stimulated activities were not affected by naloxone, naltrindol nor norbinaltorphimine which completely blocked mu-, delta- or kappa-opioid agonist-stimulated GTPgammaS binding, respectively. In addition, the distribution of nociceptin-stimulated activities throughout brain regions was found to be different from such opioid receptor-mediated ones.
Shimohira
I
I
Department of Molecular Pharmacology and Neuroscience, Nagasaki University School of Pharmaceutical Sciences, Japan.
Tokuyama
S
S
Himeno
A
A
Niwa
M
M
Ueda
H
H
D000818:Animals; D001345:Autoradiography; D001921:Brain / Q000033:anatomy & histology / Q000502:physiology; D001923:Brain Chemistry / Q000187:drug effects*; D016244:Guanosine 5'-O-(3-Thiotriphosphate) / Q000494:pharmacology*; D008297:Male; D051379:Mice; D008815:Mice, Inbred Strains; D018847:Opioid Peptides / Q000494:pharmacology*; D011957:Receptors, Opioid / Q000819:agonists*; D013462:Sulfur Radioisotopes; D000097629:Nociceptin
D016244:Guanosine 5'-O-(3-Thiotriphosphate); D018847:Opioid Peptides; D011957:Receptors, Opioid; D013462:Sulfur Radioisotopes; D000097629:Nociceptin