[Two modifications of MAIPA assays for the demonstration of platelet antibodies].
The monoclonal antibody immobilization of platelet antigens assay (MAIPA), published in 1987, is now used by many laboratories for detection of platelet antibodies. False-positive results were observed with sera containing anti-mouse IgG and in cases where MAIPA was employed to characterize platelet-specific antibodies of the IgM class. Modifications of MAIPA which completely eliminate these artifacts are described.
Kiefel
V
V
Institut für Klinische Immunologie und Transfusionsmedizin, Justus-Liebig-Universität Giessen, Deutschland.
Scheld
S
S
Freitag
E
E
Kroll
H
H
Mueller-Eckhardt
C
C
D000818:Animals; D000911:Antibodies, Monoclonal; D016824:Antigens, Human Platelet / Q000276:immunology*; D001323:Autoantibodies / Q000097:blood*; D001792:Blood Platelets / Q000276:immunology*; D005189:False Positive Reactions; D006801:Humans; D007118:Immunoassay / Q000379:methods; D007074:Immunoglobulin G / Q000097:blood; D007075:Immunoglobulin M / Q000097:blood; D051379:Mice
D000911:Antibodies, Monoclonal; D016824:Antigens, Human Platelet; D001323:Autoantibodies; D007074:Immunoglobulin G; D007075:Immunoglobulin M
Zwei Modifikationen des MAIPA-Assays zum Nachweis thrombozytärer Antikörper.