title: Determinants of kinin release in isolated rat hindquarters.
abstract: We studied the determinants of kinin release into the venous effluent of rat hindquarters perfused with Krebs bicarbonate buffer. Kinin release in preparations perfused with control media (14.6 +/- 2.5-20.7 +/- 6.7 pg/15 min) was surpassed by that in preparations perfused with media containing kininase inhibitors (243 +/- 53 to 276 +/- 78 pg/15 min). Kinin release increased when purified kininogen (from 242 +/- 43 to 3,365 +/- 725 pg/15 min) or kallikrein (from 270 +/- 49 to 30,649 +/- 8,040 pg/15 min) was added to the perfusate. Conversely, kinin release fell when the kallikrein inhibitor aprotinin (from 272 +/- 58 to 122 +/- 27 pg/15 min) or soybean trypsin inhibitor (from 273 +/- 52 to 195 +/- 25 pg/15 min) was added. Both basal and kininogen-induced kinin release were attenuated in preparations perfused with media containing cycloheximide, a protein synthesis inhibitor, but kallikrein-induced kinin release was not. These data suggest that kinin release from perfused rat hindquarters reflects the activity of both the kinin-degrading and kinin-generating pathways and that the latter is sustained by a kallikrein manufactured de novo and by preexistent kininogen(s).
authors.affiliation: Department of Physiology, New York Medical College, Valhalla 10595, USA.
authors.forename: T
authors.initials: T
authors.lastname: Sakakibara
authors.forename: T H
authors.initials: TH
authors.lastname: Hintze
authors.forename: A
authors.initials: A
authors.lastname: Nasjletti
mesh_terms: D000818:Animals; D007611:Aprotinin / Q000494:pharmacology; D003513:Cycloheximide / Q000494:pharmacology; D006614:Hindlimb / Q000098:blood supply*; D066298:In Vitro Techniques; D007610:Kallikreins / Q000494:pharmacology; D007704:Kininogens / Q000494:pharmacology; D007705:Kinins / Q000097:blood / Q000378:metabolism*; D008297:Male; D010477:Perfusion; D051381:Rats; D017207:Rats, Sprague-Dawley; D013997:Time Factors; D014361:Trypsin Inhibitors / Q000494:pharmacology
chemical_list: D007704:Kininogens; D007705:Kinins; D014361:Trypsin Inhibitors; D007611:Aprotinin; D003513:Cycloheximide; D007610:Kallikreins