Glyceraldehyde-3-phosphate dehydrogenase mRNA expression in hepatocellular carcinoma.
Glyceraldehyde-3-phosphate dehydrogenase (G3PDH) is often used as a control gene for mRNA expression, however it has been proposed to be overexpressed in all hepatocellular carcinomas (HCC). Equal amounts of tumor and paired normal (T/N) RNA, based on OD260/280 nm, were compared using ethidium bromide staining, poly-T probing, gene-specific dot blot and Northern blots using control probes G3PDH, actin and histone H4. Using mRNA blots 13/20 surgical HCC pairs did not overexpress G3PDH. Those 7/20 intact samples which did appear to overexpress G3PDH on Northern blot could not be detected by poly-T probing of dot blots. The apparent overexpression was not specific for the control gene G3PDH nor for the malignancy HCC. It may represent partial mRNA degradation, or the presence of as yet unknown substances which interfere with absorption at 260/280 nm. We advise caution in selecting human T/N pairs for differential gene expression studies. For HCC, no clinicopathological variables, including cirrhosis, predicted whether a T/N sample pair was likely to be balanced or not.
Yamagata
M
M
Medical Institute of Bioregulation, Department of Surgery, Kyushu University, Beppu, Japan.
Mori
M
M
Begum
N A
NA
Shibuta
K
K
Shimoda
K
K
Barnard
G F
GF
D006528:Carcinoma, Hepatocellular / Q000201:enzymology* / Q000473:pathology / Q000601:surgery; D017931:DNA Primers; D005260:Female; D005500:Follow-Up Studies; D005987:Glyceraldehyde-3-Phosphate Dehydrogenases / Q000096:biosynthesis*; D006801:Humans; D008099:Liver / Q000201:enzymology; D008111:Liver Function Tests; D008113:Liver Neoplasms / Q000201:enzymology* / Q000473:pathology / Q000601:surgery; D008297:Male; D008875:Middle Aged; D009361:Neoplasm Invasiveness; D015345:Oligonucleotide Probes; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000096:biosynthesis; D012016:Reference Values; D013274:Stomach Neoplasms / Q000201:enzymology; D014158:Transcription, Genetic
D017931:DNA Primers; D015345:Oligonucleotide Probes; D012333:RNA, Messenger; D005987:Glyceraldehyde-3-Phosphate Dehydrogenases