Interference due to haemolysis in routine photometric analysis--a survey.
To assess the affect of haemolysis on routine photometric methods used by participants in the Irish External Quality Assessment Scheme for Clinical Biochemistry (IEQAS) a sample was distributed with a supplied diluent containing haemoglobin. A sample of the same batch had previously been circulated as a normal distribution. Many analytes/methods were affected by the haemolysis. The range of values reported for each analyte was as follows. Albumin 34-48 g/L (baseline 37 g/L). Calcium 2.16-2.68 mmol/L (baseline 2.34 mmol/L). Urate 90-470 mumol/L (baseline 195 mumol/L). Creatinine 55-161 mumol/L (baseline 140 mumol/L). Glucose 4.1-5.6 mmol/L (baseline 4.8 mmol/L). Bilirubin 14-60 mumol/L (baseline 36 mumol/L). Urea 3.4-7.3 mmol/L (baseline 3.8 mmol/L). Iron 17.9-55.0 mumol/L (baseline 26.8 mumol/L). Inorganic phosphorus 0.22-2.41 mmol/L (baseline 1.59 mmol/L). Cholesterol 3.0-5.9 mmol/L (baseline 4.1 mmol/L). In some situations the use of an appropriate blank may solve the problem, but it must be remembered that interference caused by haemoglobin may be more complex than merely spectral.
Brady
J
J
Clinical Biochemistry Department, Our Lady's Hospital for Sick Children, Crumlin, Dublin, Ireland.
O'Leary
N
N
D000418:Albumins / Q000032:analysis; D016477:Artifacts; D001663:Bilirubin / Q000032:analysis; D002118:Calcium / Q000032:analysis; D003404:Creatinine / Q000032:analysis; D005947:Glucose / Q000032:analysis; D006461:Hemolysis / Q000502:physiology*; D007501:Iron / Q000032:analysis; D010758:Phosphorus / Q000032:analysis; D010783:Photometry / Q000592:standards*; D011786:Quality Control; D011897:Random Allocation; D014508:Urea / Q000032:analysis; D014527:Uric Acid / Q000032:analysis
D000418:Albumins; D014527:Uric Acid; D010758:Phosphorus; D014508:Urea; D003404:Creatinine; D007501:Iron; D005947:Glucose; D001663:Bilirubin; D002118:Calcium