title: Immunohistochemical localization of parathyroid hormone-related protein in developing mouse Meckel's cartilage and mandible.
abstract: In order to clarify the role of parathyroid hormone-related protein (PTHrP) during Meckel's cartilage and mandibular development, an immunohistochemical study of PTHrP and its receptor, PTH/PTHrP receptor, was designed to examine their localization in the anterior region of Meckel's cartilage including the rostrum, which is known to contribute to the development of the mandible. Meckel's cartilage was first observed on day 13 of gestation and PTHrP was faintly localized in the chondrocytes. On day 16 of gestation, at the stage of elongation and initiation of endochondral ossification in Meckel's cartilage, PTHrP was localized in the chondrocytes located in the area showing interstitial growth and in and around the nuclei of hypertrophic chondrocytes undergoing endochondral ossification. At day 18 of gestation, endochondral ossification was spread over the entire area proximal to the molar region in Meckel's cartilage, except in the mesial fusion site formed by immature chondrocytes. PTHrP was localized in the osteoblasts adjacent to the calcified matrix, but had disappeared from the chondrocytes forming Meckel's cartilage. The localization of PTH/PTHrP receptor was similar to that of PTHrP. These results show that localization of PTHrP is spatially and temporally related to the growth of Meckel's cartilage.
authors.affiliation: 2nd Department of Orthodontics, Faculty of Dentistry, Tokyo Medical and Dental University, Japan.
authors.forename: K
authors.initials: K
authors.lastname: Yamazaki
authors.forename: N
authors.initials: N
authors.lastname: Suda
authors.forename: T
authors.initials: T
authors.lastname: Kuroda
mesh_terms: D000818:Animals; D001391:Azo Compounds; D001857:Bone Matrix / Q000196:embryology; D001934:Branchial Region / Q000196:embryology; D002356:Cartilage / Q000196:embryology*; D002467:Cell Nucleus / Q000648:ultrastructure; D019902:Chondrocytes / Q000166:cytology; D004396:Coloring Agents; D005456:Fluorescent Dyes; D005865:Gestational Age; D006984:Hypertrophy; D007124:Immunoenzyme Techniques; D007150:Immunohistochemistry; D008334:Mandible / Q000166:cytology / Q000196:embryology*; D051379:Mice; D008810:Mice, Inbred C57BL; D008815:Mice, Inbred Strains; D008963:Molar / Q000196:embryology; D009432:Neural Crest / Q000196:embryology; D010006:Osteoblasts / Q000166:cytology; D010012:Osteogenesis; D010281:Parathyroid Hormone / Q000032:analysis*; D044162:Parathyroid Hormone-Related Protein; D011506:Proteins / Q000032:analysis*; D018016:Receptors, Parathyroid Hormone / Q000032:analysis
chemical_list: D001391:Azo Compounds; D004396:Coloring Agents; D005456:Fluorescent Dyes; D010281:Parathyroid Hormone; D044162:Parathyroid Hormone-Related Protein; D011506:Proteins; D018016:Receptors, Parathyroid Hormone; C005215:Fast Red S