Reduced blood accumulation of biotinylated monoclonal antibody A7 after the subsequent administration of avidin.
Clear immunoscintigraphy with radiolabeled monoclonal antibodies (MAbs) requires a high tumor tissue/blood ratio of radioactivity. In this study, we attempted to obtain a high tumor tissue/blood ratio by the active removal of radiolabeled MAb from the circulation, using the avidin-biotin system. Biotinylated 125I-labeled MAb A7 was injected intravenously into nude mice bearing a human colon cancer (WiDr) xenograft. Avidin was injected 24 h later. The tumor tissue/blood ratio of radioactivity was almost four times that of controls. These results suggest that biotinylated 125I-labeled MAb A7 and avidin are potentially useful for the rapid immunodetection of human colon cancer.
Nishi
H
H
The First Department of Surgery, Kyoto Prefectural University of Medicine, Japan.
Yamaguchi
T
T
Otsuji
E
E
Kotani
T
T
Taniguchi
K
K
Okamoto
K
K
Yata
Y
Y
Tsuruta
H
H
Takahashi
T
T
D000818:Animals; D000911:Antibodies, Monoclonal / Q000493:pharmacokinetics*; D000951:Antigens, Neoplasm / Q000276:immunology; D001360:Avidin / Q000008:administration & dosage* / Q000494:pharmacology; D001710:Biotin / Q000097:blood / Q000493:pharmacokinetics*; D003110:Colonic Neoplasms / Q000000981:diagnostic imaging* / Q000276:immunology; D006801:Humans; D007668:Kidney / Q000378:metabolism; D008099:Liver / Q000378:metabolism; D051379:Mice; D008819:Mice, Nude; D009368:Neoplasm Transplantation; D011863:Radioimmunoassay; D011877:Radionuclide Imaging; D013154:Spleen / Q000378:metabolism; D013997:Time Factors; D014407:Tumor Cells, Cultured
D000911:Antibodies, Monoclonal; D000951:Antigens, Neoplasm; D001360:Avidin; D001710:Biotin