High resolution methylation analysis of the galectin-1 gene promoter region in expressing and nonexpressing tissues.
We conducted by bisulfite genomic sequencing a high resolution study of the methylation of the galectin-1 gene in expressing and nonexpressing tissues. We show that: (i) hypomethylation of galectin-1 promoter correlates with expression; (ii) differences in methylation occur in a small region, which include a CpG cluster; (iii) the density of methyl-CpGs rather than site-specific methylation distinguishes the nonexpressing from the expressing alleles; (iv) the modification profiles in nonexpressing tissues are highly heterogeneous; (v) a single CpG within 1300 bp is always methylated both in expressing and nonexpressing tissues; (vi) these features are conserved in rat and mouse.
Salvatore
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P
Dipartimento di Biologia e Patologia Cellulare e Molecolare L. Califano, Università degli Studi di Napoli Federico II, Naples, Italy.
Benvenuto
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G
Caporaso
M
M
Bruni
C B
CB
Chiariotti
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L
D016475:3T3 Cells; D000818:Animals; D001483:Base Sequence; D002457:Cell Extracts; D018899:CpG Islands; D019175:DNA Methylation; D018076:DNA, Complementary; D004268:DNA-Binding Proteins / Q000378:metabolism; D037483:Galectin 1; D006388:Hemagglutinins / Q000235:genetics*; D051379:Mice; D008810:Mice, Inbred C57BL; D008969:Molecular Sequence Data; D009687:Nuclear Proteins / Q000378:metabolism; D011401:Promoter Regions, Genetic; D051381:Rats; D017207:Rats, Sprague-Dawley
D002457:Cell Extracts; D018076:DNA, Complementary; D004268:DNA-Binding Proteins; D037483:Galectin 1; D006388:Hemagglutinins; D009687:Nuclear Proteins