Ordered cloned DNA map of the genome of Vibrio cholerae 569B and localization of genetic markers.
By using a low-resolution macrorestriction map as the foundation (R. Majumder et al., J. Bacteriol. 176:1105-1112, 1996), an ordered cloned DNA map of the 3.2-Mb chromosome of the hypertoxinogenic strain 569B of Vibrio cholerae has been constructed. A cosmid library the size of about 4,000 clones containing more than 120 Mb of V. cholerae genomic DNA (40-genome equivalent) was generated. By combining landmark analysis and chromosome walking, the cosmid clones were assembled into 13 contigs covering about 90% of the V. cholerae genome. A total of 92 cosmid clones were assigned to the genome and to regions defined by NotI, SfiI, and CeuI macrorestriction maps. Twenty-seven cloned genes, 9 rrn operons, and 10 copies of a repetitive DNA sequence (IS1004) have been positioned on the ordered cloned DNA map.
Chatterjee
S
S
Biophysics Division, Indian Institute of Chemical Biology, Calcutta.
Mondal
A K
AK
Begum
N A
NA
Roychoudhury
S
S
Das
J
J
D002772:Cholera Toxin / Q000096:biosynthesis; D003001:Cloning, Molecular; D003360:Cosmids; D004269:DNA, Bacterial / Q000235:genetics; D005798:Genes, Bacterial; D005819:Genetic Markers; D016680:Genome, Bacterial; D015698:Genomic Library; D015183:Restriction Mapping; D013045:Species Specificity; D014734:Vibrio cholerae / Q000145:classification / Q000235:genetics*
D004269:DNA, Bacterial; D005819:Genetic Markers; D002772:Cholera Toxin