Analysis of heterocyclic aromatic amines in wine by high-performance liquid chromatography-electrospray tandem mass spectrometry.
High-performance liquid chromatography-electrospray tandem mass spectrometry (HPLC-ESI-MS-MS) was used for the analysis of heterocyclic aromatic amines (HAAs) in 25 wines from various regions. In 24 wines under study HAAs were detected in the low ng/l range. 2-Amino-3,4-dimethyl-3H-imidazo[4,5-f]quinoxaline (MeIQx) and 2-amino-3-methyl-9H-pyrido[2,3-b]indole (MeA alpha C) were found to be the most widely distributed HAAs in the wines under study. There was a difference in the presence of HAAs in white and red wines. In the latter, higher concentrations were found, with MeA alpha C in amount ranging from < 7.5 to 107 ng/l. Compared to the quantities of HAAs evaluated in meat and meat products, the amounts found in wines are 100-1000-fold lower.
Richling
E
E
Lehrstuhl für Lebensmittelchemie, Universität Würzburg, Germany.
Decker
C
C
Häring
D
D
Herderich
M
M
Schreier
P
P
D000588:Amines / Q000032:analysis* / Q000737:chemistry; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D006571:Heterocyclic Compounds / Q000032:analysis* / Q000737:chemistry; D013058:Mass Spectrometry / Q000379:methods*; D012680:Sensitivity and Specificity; D014920:Wine / Q000032:analysis*
D000588:Amines; D006571:Heterocyclic Compounds