Polypyrrole DNA chip on a silicon device: example of hepatitis C virus genotyping.
We describe in this article an oligonucleotide array constructed on a silicon device bearing a matrix of addressable 50-microns microelectrodes. Each electrode was covered by a conducting polymer (polypyrrole) grafted by an oligonucleotide (ODN). The DNA chip was prepared by successive electrochemically addressed copolymerizations of 5' pyrrole-labeled ODN and pyrrole. Following hybridization of the biotinylated amplified sample on the chip bearing a series of probes, detection was carried out by fluorescence microscopy through an R-phycoerythrin label. This technology was successfully applied to the genotyping of hepatitis C virus in blood samples. Results show good sensitivity and a high degree of dimensional resolution.
Livache
T
T
CIS Bio International, DIVT, Bagnols/Céze, France.
Fouque
B
B
Roget
A
A
Marchand
J
J
Bidan
G
G
Téoule
R
R
Mathis
G
G
D001709:Biotechnology / Q000295:instrumentation*; D004247:DNA / Q000737:chemistry* / Q000235:genetics; D004279:DNA, Viral / Q000032:analysis*; D004563:Electrochemistry; D005838:Genotype; D016174:Hepacivirus / Q000235:genetics*; D008839:Microelectrodes; D009693:Nucleic Acid Hybridization; D009841:Oligonucleotides / Q000737:chemistry; D011758:Pyrroles / Q000737:chemistry*; D011786:Quality Control; D012680:Sensitivity and Specificity; D012825:Silicon
D004279:DNA, Viral; D009841:Oligonucleotides; D011758:Pyrroles; D004247:DNA; D012825:Silicon