title: Over-expression of the yeast BFR2 gene partially suppresses the growth defects induced by Brefeldin A and by four ER-to-Golgi mutations.
abstract: The fungal metabolite Brefeldin A (BFA) disrupts the Golgi apparatus and its incoming protein flux. We developed a genetic approach to identify yeast proteins involved in the protein transport step that BFA blocks. The BFR2 gene (YDR299W) was thus isolated as a high-copy suppressor of the growth defects induced by BFA in a sensitive strain of Saccharomyces cerevisiae. Although BFR2 over-expression did not cause a secretory block or slow-down, it partially suppressed the growth defect of four mutants blocked at the step of budding or docking of small vesicles en route to the Golgi (sec13-1, sec16-2, sec23-1, ypt1-1). The essential BFR2 gene was predicted to encode an extremely hydrophilic product containing two short regions with potential coiled-coils, one of which corresponds to a cluster of acidic residues.
authors.affiliation: Service de Biochimie et de Génétique Moléculaire, DBCM/DSV, Bât. 142, CEA/Saclay, F-91191 Gif cedex, France.
authors.forename: S
authors.initials: S
authors.lastname: Chabane
authors.forename: E
authors.initials: E
authors.lastname: Gachet
authors.forename: F
authors.initials: F
authors.lastname: Képès
mesh_terms: D000935:Antifungal Agents / Q000494:pharmacology; D020126:Brefeldin A; D003517:Cyclopentanes / Q000494:pharmacology*; D004721:Endoplasmic Reticulum / Q000235:genetics* / Q000378:metabolism; D015966:Gene Expression Regulation, Fungal / Q000187:drug effects*; D005800:Genes, Fungal / Q000187:drug effects; D016153:Genes, Suppressor / Q000187:drug effects*; D006056:Golgi Apparatus / Q000235:genetics* / Q000378:metabolism; D009154:Mutation; D009761:Nystatin / Q000494:pharmacology; D015388:Organelles / Q000235:genetics / Q000378:metabolism; D017468:Receptors, Fibroblast Growth Factor / Q000096:biosynthesis / Q000235:genetics*; D012441:Saccharomyces cerevisiae / Q000187:drug effects / Q000235:genetics* / Q000254:growth & development*; D029701:Saccharomyces cerevisiae Proteins
chemical_list: D000935:Antifungal Agents; C069491:BFR2 protein, S cerevisiae; D003517:Cyclopentanes; D017468:Receptors, Fibroblast Growth Factor; D029701:Saccharomyces cerevisiae Proteins; D009761:Nystatin; D020126:Brefeldin A