Action of extremely low frequency electric fields on the cytosolic calcium concentration of differentiated HL-60 cells: nonactivated cells.
The effect of sinusoidal electric fields on the cytosolic free [Ca2+]i concentration in differentiated HL-60 cells was measured. The calcium concentration was measured in a fluorescence spectrometer using the fluorescence sample fluo-3. In the fluorescence spectrometer two samples can be measured simultaneously, one as the sham-exposed control and the other as the field-exposed sample. The effects of an external field, applied using two capacitor plates outside the cuvettes, and a field applied directly to the medium, using two platinum electrodes inside the cuvettes, were measured at selected frequencies between 0 and 100 Hz and field strengths from 1 to 2000 Vpp/m (external field) and from 0.1 to 1000 Vpp/m (in medium). No significant effects of the fields on the cytosolic free [Ca2+]i concentration in HL-60 cells have been observed at the measured frequencies and field strengths.
Sontag
W
W
Forschungszentrum Karlsruhe, Institut für Toxikologie, Germany. sontag@itox.fzk.de
D002118:Calcium / Q000378:metabolism*; D002454:Cell Differentiation; D003600:Cytosol / Q000378:metabolism / Q000528:radiation effects; D004307:Dose-Response Relationship, Radiation; D004574:Electromagnetic Fields; D018922:HL-60 Cells / Q000378:metabolism / Q000528:radiation effects; D006801:Humans; D007700:Kinetics
D002118:Calcium