Structural analysis of hexa to dodecaoligosaccharide-alditols released by reductive beta-elimination from oviducal mucins of Bufo bufo.
Hexa to dodecasaccharide-alditols of the jelly coat surrounding the eggs of the toad Bufo bufo were studied by methylation analysis, MALDI-TOF mass spectrometry, and H-NMR spectroscopy. These highly species-specific carbohydrate chains exhibit new structural features, such as the elongation of the blood group A determinant with an external alpha-1,3-linked galactose unit, or ramification belonging from a fucosylated galactose. The most representative oligosaccharide-alditol of the series was defined as following: [structure in text]. Since the jellies surrounding amphibian eggs are involved in egg-sperm interactions, these structural investigations can provide biochemical support for exploring the fertilization process.
Morelle
W
W
Unité Mixte de Recherche du CNRS 111, Université des Sciences et Technologies de Lille, Villeneuve d'Ascq, France.
Strecker
G
G
D000818:Animals; D002023:Bufo bufo; D002236:Carbohydrate Conformation; D002240:Carbohydrate Sequence; D002851:Chromatography, High Pressure Liquid; D005260:Female; D009682:Magnetic Resonance Spectroscopy; D013058:Mass Spectrometry; D008745:Methylation; D008969:Molecular Sequence Data; D009077:Mucins / Q000737:chemistry*; D009844:Oligosaccharides / Q000737:chemistry*; D010057:Oviducts / Q000737:chemistry; D010063:Ovum / Q000737:chemistry; D013402:Sugar Alcohols / Q000737:chemistry*
D009077:Mucins; D009844:Oligosaccharides; D013402:Sugar Alcohols