title: Changes in polymorphonuclear leukocytes in the vagina of patients with preterm labor.
abstract: To evaluate changes in vaginal polymorphonuclear leukocytes (vPMNL) in patients with preterm labor, we obtained vPMNL-rich suspensions from vaginal fluids of 77 women (21 with preterm labor and 56 control women with uncomplicated pregnancy). Total counts of vPMNL and % viability of vPMNL (viable vPMNL:total vPMNL) were significantly higher in patients with preterm labor (37 +/- 92 x 10(5), 23 +/- 29%) vs. controls (11 +/- 15 x 10(5), 6.9 +/- 8.3%). The concentration of granulocyte elastase in vaginal fluid diluted to 20 ml was also higher in patients with preterm labor than in controls (4,806 +/- 3,818 vs. 2,739 +/- 2,556 ng/ml). We conclude that PMNL and granulocyte elastase are abundant in the vagina in patients with preterm labor, which suggests that the PMNL may be involved in the pathogenesis of preterm labor.
authors.affiliation: Department of Obstetrics and Gynecology, Jichi Medical School, Togichi, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Yamada
authors.forename: H
authors.initials: H
authors.lastname: Minakami
authors.forename: S
authors.initials: S
authors.lastname: Matsubara
authors.forename: T
authors.initials: T
authors.lastname: Yatsuda
authors.forename: I
authors.initials: I
authors.lastname: Sato
mesh_terms: D000328:Adult; D002470:Cell Survival; D015331:Cohort Studies; D005260:Female; D006801:Humans; D007958:Leukocyte Count; D019272:Leukocyte Elastase / Q000032:analysis; D008854:Microscopy, Electron; D009504:Neutrophils / Q000473:pathology* / Q000648:ultrastructure; D007752:Obstetric Labor, Premature / Q000097:blood / Q000473:pathology*; D011247:Pregnancy; D012016:Reference Values; D014621:Vagina / Q000201:enzymology / Q000378:metabolism / Q000473:pathology*
chemical_list: D019272:Leukocyte Elastase