[Biparametric analysis of the apoptotic index and the proliferation index (KI-67) in solid tumors].
BACKGROUND
Cell kinetic data are important indicators of the aggressiveness of tumor and treatment response. In this study biparametric analysis of proliferation and apoptotic index was performed on paraffin-embedded tissue from certain neoplasia.


METHODS
We studied 107 cases of neoplastic and non neoplastic lesions. For each case, on the same section, TUNEL reaction and immunohistochemical protein Ki67 expression analysis were performed.


RESULTS
On 102 out of 107 cases (95.3%) we obtained valid results both about apoptotic and proliferative indices.


CONCLUSIONS
This method could supply, at the same time, interesting information about the two possible different patterns of neoplastic growth: one related to an increase fraction of proliferating cells, the other to decrease in apoptotic cell death. Cell kinetic data may have interesting implications on therapeutic choice.
Mangili
F
F
Servizio di Anatomia e Istologia Patologica, Istituto Scientifico H San Raffaele, Milano.
Cigala
C
C
Gattuso
C
C
D000951:Antigens, Neoplasm / Q000032:analysis*; D017209:Apoptosis; D015415:Biomarkers; D002289:Carcinoma, Non-Small-Cell Lung / Q000473:pathology; D002292:Carcinoma, Renal Cell / Q000473:pathology; D002453:Cell Cycle; D002455:Cell Division; D053938:DNA Fragmentation; D006509:Hepatitis B / Q000473:pathology; D006526:Hepatitis C / Q000473:pathology; D006521:Hepatitis, Chronic / Q000473:pathology; D006801:Humans; D019394:Ki-67 Antigen / Q000032:analysis*; D007680:Kidney Neoplasms / Q000473:pathology; D008099:Liver / Q000473:pathology; D008175:Lung Neoplasms / Q000473:pathology; D008940:Mitotic Index; D009369:Neoplasms / Q000473:pathology*; D016612:Paraffin Embedding; D011933:Reagent Kits, Diagnostic
D000951:Antigens, Neoplasm; D015415:Biomarkers; D019394:Ki-67 Antigen; D011933:Reagent Kits, Diagnostic
Analisi biparametrica di indice apoptotico e indice di proliferazione (KI-67) nei tumori solidi.