Polymorphonuclear elastase in human colorectal carcinoma.
proteinases are required for invasion through the extracellular matrix (ECM) during tumor invasion and metastasis. Polymorphonuclear (PMN) elastase can degrade ECM components and modulate other proteinases. In the present study PMN elastase activity was found in tissue extracts from 8 of 15 human colorectal carcinomas. Immunoreactive PMN elastase was demonstrated in all carcinoma biopsies with particular enrichment at the tumor-host interface. Immunofluorescence staining localized immunoreactive PMN elastase mainly to neutrophile granulocytes. The human colon carcinoma cell lines Caco-2 and HT-29 did not express PMN elastase. An interaction between tumor cells and elastase producing leukocytes is suggested.
Bjørnland
K
K
Institute for Surgical Research, The National Hospital, 0027 Oslo, Norway.
Buø
L
L
Scott
H
H
Konttinen
Y
Y
Johansen
H T
HT
Aasen
A O
AO
D000230:Adenocarcinoma / Q000201:enzymology* / Q000473:pathology*; D000368:Aged; D003110:Colonic Neoplasms; D015179:Colorectal Neoplasms / Q000201:enzymology* / Q000473:pathology*; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D019272:Leukocyte Elastase / Q000032:analysis*; D008297:Male; D008875:Middle Aged; D009504:Neutrophils / Q000201:enzymology / Q000473:pathology; D014407:Tumor Cells, Cultured
D019272:Leukocyte Elastase