title: Phylogenetic analysis of 8 dermatophyte species using chitin synthase 1 gene sequences.
abstract: Nucleotide sequences of chitin synthase 1 (CHS1) gene of eight species of dermatophytes, Arthroderma benhamiae, A. fulvum, A. grubyi, A. gypseum, A. incurvatum, A. otae, A. simii and A. vanbreuseghemii were obtained and analysed for their phylogenetic relationship. A 600-bp genomic DNA fragment of the CHS1 gene was amplified from these dermatophytes by polymerase chain reaction (PCR) and sequenced. The CHS1 nucleotide sequences of these eight dermatophyte species showed more than 85% similarity between the species. The phylogenetic analysis of their sequences revealed three clusters, the first cluster consisting of A. benhamiae, A. simii and A. vanbreuseghemii, the second cluster consisting of A. fulvum, A. gypseum and A. incurvatum, and the third cluster consisting of A. grubyi and A. otae. The phylogenetic analysis of CHS1 gene in this study will provide useful information for classification and understanding the evolution of these dermatophyte species.
authors.affiliation: Department of Veterinary Internal Medicine, Graduate School of Agricultural and Life Sciences, University of Tokyo, Japan.
authors.forename: R
authors.initials: R
authors.lastname: Kano
authors.forename: Y
authors.initials: Y
authors.lastname: Nakamura
authors.forename: T
authors.initials: T
authors.lastname: Watari
authors.forename: S
authors.initials: S
authors.lastname: Watanabe
authors.forename: H
authors.initials: H
authors.lastname: Takahashi
authors.forename: H
authors.initials: H
authors.lastname: Tsujimoto
authors.forename: A
authors.initials: A
authors.lastname: Hasegawa
mesh_terms: D003883:Arthrodermataceae / Q000145:classification* / Q000201:enzymology / Q000235:genetics; D001203:Ascomycota / Q000145:classification* / Q000201:enzymology / Q000235:genetics; D002687:Chitin Synthase / Q000235:genetics*; D003001:Cloning, Molecular; D004271:DNA, Fungal; D019143:Evolution, Molecular; D005800:Genes, Fungal; D016533:Mycological Typing Techniques; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D017422:Sequence Analysis, DNA
chemical_list: D004271:DNA, Fungal; D002687:Chitin Synthase