title: Separation of alditols of interest in food products by high-performance anion-exchange chromatography with pulsed amperometric detection.
abstract: High-performance anion-exchange chromatography (HPAEC)-pulsed amperometric detection (PAD) employing a CarboPac MA 1 column was investigated with respect to mobile phase composition, linear response characteristics, repeatability, reproducibility and sensitivity with different alditols used as sugar substitutes in food and confectionery products. The energy-reduced bulk sweeteners isomalt and maltitol were well resolved in less than 25 min by isocratic elution with 600 mM sodium hydroxide solution. HPAEC-PAD was also successfully applied to the determination of alditols in sugar-free products and a low-calorie sweetener containing sorbitol, mannitol and fructose at different levels.
authors.affiliation: Istituto di Cromatografia del C.N.R., Rome, Italy.
authors.forename: C
authors.initials: C
authors.lastname: Corradini
authors.forename: G
authors.initials: G
authors.lastname: Canali
authors.forename: E
authors.initials: E
authors.lastname: Cogliandro
authors.forename: I
authors.initials: I
authors.lastname: Nicoletti
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004563:Electrochemistry; D005632:Fructose / Q000302:isolation & purification; D008353:Mannitol / Q000302:isolation & purification; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013012:Sorbitol / Q000302:isolation & purification; D013402:Sugar Alcohols / Q000302:isolation & purification*; D013549:Sweetening Agents / Q000737:chemistry*; D013997:Time Factors
chemical_list: D013402:Sugar Alcohols; D013549:Sweetening Agents; D005632:Fructose; D008353:Mannitol; D013012:Sorbitol