Molecular cloning and characterization of rat lin-10.
In Caenorhabditis elegans, the vulval induction is mediated by tyrosine kinase receptor/Ras signal transduction pathway composed of the lin-3, let-23, and let-60 products. In addition to these gene products, the lin-2, lin-7, and lin-10 products are also implicated in this pathway. Lin-2 encodes a MAGUK and lin-7 encodes a small protein with one PDZ domain. The lin-10 product has no homology to known proteins. Here, we have cloned a rat homologue of the lin-10 product and characterized it. Rat lin-10 is ubiquitously expressed in various rat tissues and distributed in both the cytosol and membrane fractions. In brain, however, rat lin-10 is distributed only in the membrane fraction and enriched in the synaptic plasma membrane and postsynaptic density fractions. These results suggest that rat lin-10 is involved at least in synaptic functions in brain.
Ide
N
N
Takai Biotimer Project, ERATO, Japan Science and Technology Corporation, Kobe, Japan.
Hirao
K
K
Hata
Y
Y
Takeuchi
M
M
Irie
M
M
Yao
I
I
Deguchi
M
M
Toyoda
A
A
Nishioka
H
H
Mizoguchi
A
A
Takai
Y
Y
D000818:Animals; D017173:Caenorhabditis elegans / Q000737:chemistry; D029742:Caenorhabditis elegans Proteins; D002478:Cells, Cultured; D003001:Cloning, Molecular; D018507:Gene Expression Regulation, Developmental / Q000235:genetics; D015801:Helminth Proteins / Q000032:analysis / Q000737:chemistry*; D006624:Hippocampus / Q000737:chemistry; D007150:Immunohistochemistry; D008565:Membrane Proteins; D009419:Nerve Tissue Proteins / Q000032:analysis / Q000737:chemistry; D011506:Proteins; D012333:RNA, Messenger / Q000032:analysis; D051381:Rats; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D013569:Synapses / Q000502:physiology
D029742:Caenorhabditis elegans Proteins; D015801:Helminth Proteins; D008565:Membrane Proteins; D009419:Nerve Tissue Proteins; D011506:Proteins; D012333:RNA, Messenger; C064105:lin-10 protein, C elegans