Enantiomeric separation of d-/l-norepinephrine and -epinephrine by high-performance liquid chromatography with a beta-cyclodextrin type chiral stationary phase.
Enantiomeric separation of d-/l-norepinephrine (NE) and d-/l-epinephrine (E) was investigated with various mobile phases by high-performance liquid chromatography using a beta-cyclodextrin type chiral stationary phase as a chiral column. The mobile phase of 2.5 M phosphate buffer (pH 3.0) gave separation factors (alpha s) of 1.04 and 1.06 for NE and E, respectively.
Fukushima
T
T
Faculty of Pharmaceutical Sciences, University of Tokyo, Japan.
Murayama
K
K
Santa
T
T
Homma
H
H
Imai
K
K
D002021:Buffers; D002846:Chromatography, Affinity; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D003505:Cyclodextrins; D004837:Epinephrine / Q000737:chemistry / Q000302:isolation & purification*; D006863:Hydrogen-Ion Concentration; D009638:Norepinephrine / Q000737:chemistry / Q000302:isolation & purification*; D013237:Stereoisomerism; D047392:beta-Cyclodextrins
D002021:Buffers; D003505:Cyclodextrins; D047392:beta-Cyclodextrins; C031215:betadex; D009638:Norepinephrine; D004837:Epinephrine