B1 bradykinin receptors and carboxypeptidase M are both upregulated in the aorta of pigs after LPS infusion.
Bradykinin receptor subtypes were characterized in aortic cryosections obtained from healthy normal pigs, animals that were given an LPS infusion, and animals that came with a pre-existing infection or inflammation to the laboratory by binding studies and in vitro autoradiography. In control aorta a single class of high affinity B2 binding sites, located within the endothelium, but with no significant binding of B1 ligand were identified. No major changes in the expression of B2 BK receptors were noted in inflammed tissues. In cryosections of inflammed vascular tissue a markedly increased endothelial carboxypeptidase M activity was verified that parallelled an upregulation of B1 receptors in the aortic smooth muscle layer. In crosstalk between endothelial cells and smooth muscle cells B1 receptor mediated functional responses may counteract some of the detrimental effects of inflammation.
Schremmer-Danninger
E
E
Abteilung für Klinische Chemie, Ludwig-Maximilians-Universität, Klinikum Innenstadt, Münich, Germany.
Offner
A
A
Siebeck
M
M
Roscher
A A
AA
D000818:Animals; D001013:Aorta, Thoracic / Q000187:drug effects* / Q000378:metabolism*; D001025:Aortitis / Q000139:chemically induced / Q000378:metabolism; D001345:Autoradiography; D004730:Endothelium, Vascular / Q000187:drug effects / Q000378:metabolism; D058851:GPI-Linked Proteins; D066298:In Vitro Techniques; D007700:Kinetics; D008024:Ligands; D008070:Lipopolysaccharides / Q000633:toxicity*; D008666:Metalloendopeptidases / Q000378:metabolism*; D009131:Muscle, Smooth, Vascular / Q000187:drug effects / Q000378:metabolism; D043783:Receptor, Bradykinin B1; D043782:Receptor, Bradykinin B2; D018002:Receptors, Bradykinin / Q000819:agonists / Q000378:metabolism*; D013552:Swine; D015854:Up-Regulation / Q000187:drug effects
D058851:GPI-Linked Proteins; D008024:Ligands; D008070:Lipopolysaccharides; D043783:Receptor, Bradykinin B1; D043782:Receptor, Bradykinin B2; D018002:Receptors, Bradykinin; C058479:carboxypeptidase M; D008666:Metalloendopeptidases