Translational activation and cytoplasmic polyadenylation of FGF receptor-1 are independently regulated during Xenopus oocyte maturation.
FGF signaling is critical for establishing the Xenopus laevis embryonic body plan and requires the expression of functional FGF receptor during early embryogenesis. FGF receptor-1 (XFGFR) maternal mRNA is present in immature oocytes, but the protein is not expressed until oocyte maturation. In this report we demonstrate that endogenous XFGFR translation begins just prior to germinal vesicle breakdown and that translation depends on completion of earlier meiotic events. We show that the previously identified XFGFR 3'UTR translation inhibitory element (TIE), which is necessary and sufficient for repressing translation in the immature oocyte, also regulates the onset of translation during oocyte maturation. In addition we demonstrate that cytoplasmic polyadenylation of XFGFR RNA is regulated independently of TIE-mediated translation and it not sufficient to activate the translation of XFGFR. These experiments reveal that polyadenylation and translational activation are separable events in this mRNA, each of which is timed and regulated independently.
Culp
P A
PA
Department of Obstetrics, Gynecology, and Reproductive Sciences, University of California School of Medicine 94143-0556, USA.
Musci
T J
TJ
D000818:Animals; D003593:Cytoplasm / Q000378:metabolism*; D004274:DNA, Recombinant; D018507:Gene Expression Regulation, Developmental / Q000235:genetics; D016258:Genes, mos / Q000235:genetics; D008540:Meiosis / Q000502:physiology; D008845:Microinjections; D008957:Models, Genetic; D009865:Oocytes / Q000254:growth & development* / Q000378:metabolism; D011061:Poly A / Q000378:metabolism; D011374:Progesterone / Q000494:pharmacology; D014176:Protein Biosynthesis / Q000235:genetics / Q000502:physiology*; D012313:RNA; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism*; D017468:Receptors, Fibroblast Growth Factor / Q000032:analysis / Q000235:genetics*; D012045:Regulatory Sequences, Nucleic Acid / Q000502:physiology; D017384:Sequence Deletion; D014982:Xenopus laevis
D004274:DNA, Recombinant; D012333:RNA, Messenger; C039919:RNA, recombinant; D017468:Receptors, Fibroblast Growth Factor; D011061:Poly A; D011374:Progesterone; D012313:RNA