title: Assessment of enzyme linked immunosorbent assay based diagnostic kits (Toxokit-G and Toxokit-M) for the detection of IgG and IgM antibodies to Toxoplasma gondii in human serum.
abstract: An enzyme linked immunosorbent assay (ELISA) using penicillinase was developed in the form of diagnostic kits (Toxokit-G and Toxokit-M) for the detection of IgG and IgM antibodies to Toxoplasma gondii. The performance of both the kits was compared with commercially available diagnostic kits, i.e. Enzygnost-Toxoplasmosis/IgG (Behring Co., Germany), TOXOTEK-G (Flow Lab., U.K.) and Toxoplasma IgM Microassay (Diamedix Corp., U.S.A.) by testing toxoplasma-suspected human serum samples. The results indicate a good reliability between these diagnostic kits. Toxokit-G has 86.66 and 96.05% sensitivity and specificity respectively. The main advantage of Toxokit-G is that the end result can be assessed visually without using sophisticated instruments. Toxokit-M has 100% sensitivity and specificity and test results were not affected by the presence of antitoxoplasma IgG antibodies, rheumatoid factor or antinuclear antibodies.
authors.affiliation: Laboratory of Parasitology and Immunodiagnostics, Hindustan Antibiotics Ltd, Pimpri, Pune, India.
authors.forename: G M
authors.initials: GM
authors.lastname: Bhopale
authors.forename: S R
authors.initials: SR
authors.lastname: Naik
authors.forename: G G
authors.initials: GG
authors.lastname: Bhave
authors.forename: S S
authors.initials: SS
authors.lastname: Naik
authors.forename: A
authors.initials: A
authors.lastname: Gogate
mesh_terms: D000818:Animals; D000913:Antibodies, Protozoan / Q000097:blood*; D004311:Double-Blind Method; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D007074:Immunoglobulin G / Q000097:blood*; D007075:Immunoglobulin M / Q000097:blood*; D010405:Penicillinase / Q000737:chemistry; D011933:Reagent Kits, Diagnostic / Q000592:standards*; D014122:Toxoplasma / Q000276:immunology*; D014123:Toxoplasmosis / Q000175:diagnosis* / Q000276:immunology
chemical_list: D000913:Antibodies, Protozoan; D007074:Immunoglobulin G; D007075:Immunoglobulin M; D011933:Reagent Kits, Diagnostic; D010405:Penicillinase