Surfactant protein A is decreased in a rat model of congenital diaphragmatic hernia.
We hypothesized that the expression of surfactant protein A (SP-A) would be altered in developing lungs from rat fetuses with congenital diaphragmatic hernia (CDH) induced by maternal ingestion of 2,4-dichlorophenyl-p-nitrophenyl ether (Nitrofen) on Day 9 of gestation. We compared our findings in fetuses exposed to Nitrofen with a CDH with those in Nitrofen-exposed fetuses without a CDH, and control fetuses whose mothers received olive oil only, the vehicle for Nitrofen. In late gestation, immunocytochemistry using a polyclonal rabbit antihuman SP-A antibody revealed decreased amounts of this protein in lungs from fetuses with CDH. Using immunoblotting, the relative amount of SP-A on Day 21 of gestation was also decreased in lung tissue from fetuses with CDH compared with the other groups. Abnormalities of mRNA for SP-A were observed in both groups of Nitrofen-exposed fetuses compared with control rats. These findings suggest that there is decreased expression of SP-A in rat fetuses with CDH secondary to Nitrofen exposure.
Mysore
M R
MR
Department of Pediatrics, University of Texas Southwestern Medical Center, Dallas, USA.
Margraf
L R
LR
Jaramillo
M A
MA
Breed
D R
DR
Chau
V L
VL
Arévalo
M
M
Moya
F R
FR
D000818:Animals; D015152:Blotting, Northern; D004622:Embryo, Mammalian / Q000378:metabolism; D005314:Embryonic and Fetal Development / Q000502:physiology; D006548:Hernia, Diaphragmatic / Q000196:embryology / Q000378:metabolism*; D065630:Hernias, Diaphragmatic, Congenital; D015151:Immunoblotting; D007150:Immunohistochemistry; D008168:Lung / Q000196:embryology / Q000378:metabolism; D011510:Proteolipids / Q000235:genetics / Q000378:metabolism*; D037662:Pulmonary Surfactant-Associated Protein A; D037661:Pulmonary Surfactant-Associated Proteins; D011663:Pulmonary Surfactants / Q000235:genetics / Q000378:metabolism*; D012333:RNA, Messenger / Q000378:metabolism; D011817:Rabbits; D051381:Rats / Q000196:embryology; D017207:Rats, Sprague-Dawley
D011510:Proteolipids; D037662:Pulmonary Surfactant-Associated Protein A; D037661:Pulmonary Surfactant-Associated Proteins; D011663:Pulmonary Surfactants; D012333:RNA, Messenger