Characterisation of an outer membrane protein of Moraxella catarrhalis.
To elucidate potential vaccine antigens, Moraxella catarrhalis outer membrane proteins (OMPs) were studied. We have previously shown an OMP to be a target for human IgG and have now further characterised this OMP which appears to have a molecular mass of 84 kDa and to be distinct from the 81-kDa OMP, CopB. Human transferrin was shown to bind the 84-kDa OMP alone. N-terminal sequencing of this OMP and purified M. catarrhalis transferrin binding protein B (TbpB) revealed homology both with each other and with the TbpB of Haemophilus influenzae and Neisseria meningitidis. Adsorption of human anti-serum with purified TbpB from two M. catarrhalis strains abolished or reduced binding of IgG to the 84-kDa OMP from three M. catarrhalis isolates. IgG binding to CopB was unaffected. It is clear that the 84-kDa OMP is distinct from CopB and is a likely homologue of TbpB.
Mathers
K E
KE
Immunobiology Unit, Institute of Child Health, London, UK.
Goldblatt
D
D
Aebi
C
C
Yu
R
R
Schryvers
A B
AB
Hansen
E J
EJ
D000328:Adult; D001425:Bacterial Outer Membrane Proteins / Q000032:analysis*; D006801:Humans; D008970:Molecular Weight; D001936:Moraxella catarrhalis / Q000737:chemistry*
D001425:Bacterial Outer Membrane Proteins