Purification and characterization of cytochrome c6 from the unicellular green alga Scenedesmus obliquus.
Purification of a soluble cytochrome c6 from the unicellular green alga Scenedesmus obliquus by a simple and rapid method is described. The purification procedure includes ammonium sulfate precipitation and non-denaturating PAGE. The N-terminal sequence of the first 20 amino acids was determined and shows 85% similarity and 75% identity to the sequence of cytochrome c6 from the green alga Monoraphidium braunii. The ferrocytochrome shows typical UV/VIS absorption peaks at 552.9, 521.9 and 415.7 nm. The apparent molecular mass was estimated to be 12 kDa by SDS-PAGE. EPR-spectroscopy at 20 K shows resonances indicative for two distinct low-spin heme forms.
Wünschiers
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R
Fachbereich Biologie/Botanik, Philipps-Universität, Marburg, Germany.
Zinn
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T
Linder
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Schulz
R
R
D000595:Amino Acid Sequence; D000460:Chlorophyta / Q000378:metabolism*; D003580:Cytochromes / Q000737:chemistry* / Q000302:isolation & purification; D045348:Cytochromes f; D004591:Electrophoresis, Polyacrylamide Gel; D006418:Heme / Q000032:analysis; D008969:Molecular Sequence Data; D010446:Peptide Fragments / Q000737:chemistry / Q000302:isolation & purification; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid; D013053:Spectrophotometry
D003580:Cytochromes; D010446:Peptide Fragments; D006418:Heme; D045348:Cytochromes f