Cellular localization and expression of the serotonin transporter in mouse brain.
The high-affinity serotonin (5-HT) transporter (5-HTT) plays an important role in the removal of extracellular serotonin, thereby modulating and terminating the action of this neurotransmitter at various pre- and post-synaptic serotonergic receptors and heteroreceptors. In order to characterize the anatomical distribution of the 5-HTT in mouse brain, in situ hybridization histochemistry using 35S-labeled riboprobes was performed. These results were compared with 5-HTT binding site distribution as evaluated by [125I]RTI-55 autoradiography. High levels of 5-HTT mRNA were detected in all brain stem raphe nuclei, with variations in labeling among the various subnuclei. Those brain areas known to possess serotonergic cell bodies stained intensely for both 5-HTT mRNA and 5-HTT binding sites. In contrast to previous findings in rat brain, the highest densities of 5-HTT sites were found in areas outside the raphe complex, particularly in the substantia nigra, globus pallidus, and superior colliculi.
Bengel
D
D
Section on Clinical Neuropharmacology, Laboratory of Clinical Science, NIMH, NIH Clinical Center, Bethesda, MD 20892, USA. dietmar@codon.nih.gov
Jöhren
O
O
Andrews
A M
AM
Heils
A
A
Mössner
R
R
Sanvitto
G L
GL
Saavedra
J M
JM
Lesch
K P
KP
Murphy
D L
DL
D000818:Animals; D001923:Brain Chemistry / Q000502:physiology*; D002352:Carrier Proteins / Q000032:analysis / Q000235:genetics*; D002531:Cerebellum / Q000737:chemistry; D003001:Cloning, Molecular; D015870:Gene Expression; D005917:Globus Pallidus / Q000737:chemistry; D017403:In Situ Hybridization; D007457:Iodine Radioisotopes; D008297:Male; D008562:Membrane Glycoproteins / Q000032:analysis / Q000235:genetics*; D026901:Membrane Transport Proteins; D051379:Mice; D009419:Nerve Tissue Proteins / Q000032:analysis / Q000235:genetics; D015347:RNA Probes; D012333:RNA, Messenger / Q000032:analysis; D011903:Raphe Nuclei / Q000737:chemistry; D050486:Serotonin Plasma Membrane Transport Proteins; D013378:Substantia Nigra / Q000737:chemistry; D013477:Superior Colliculi / Q000737:chemistry
D002352:Carrier Proteins; D007457:Iodine Radioisotopes; D008562:Membrane Glycoproteins; D026901:Membrane Transport Proteins; D009419:Nerve Tissue Proteins; D015347:RNA Probes; D012333:RNA, Messenger; D050486:Serotonin Plasma Membrane Transport Proteins; C493231:Slc6a4 protein, mouse