title: Characterization of a simian T-lymphotropic virus from a wild-caught orang-utan (Pongo pygmaeus) from Kalimantan, Indonesia.
abstract: In a recent serological survey among 143 ex-captive orang-utans two individuals were found that reacted positive in an ELISA detecting antibodies which cross-react with human T-lymphotropic virus type I (HTLV-I) antigens. Infection of both animals with an HTLV-I or simian T-lymphotropic virus (STLV)-like virus was confirmed by Western blot analysis. A third wild-caught animal, which was not part of the original serological survey, was also found to be infected with an HTLV-related virus in a diagnostic PCR assay and Western blot assay. Nucleotide sequence analysis of the 709 bp PCR fragment from the tax/rex region of the HTLV/STLV genome confirmed infection of orang-utans with an STLV similar to but clearly distinct from other Asian STLVs.
authors.affiliation: Department of Virology, Biomedical Primate Research Centre, Rijswijk, The Netherlands. verschoor@bprc.nl
authors.forename: E J
authors.initials: EJ
authors.lastname: Verschoor
authors.forename: K S
authors.initials: KS
authors.lastname: Warren
authors.forename: H
authors.initials: H
authors.lastname: Niphuis
authors.lastname: Heriyanto
authors.forename: R A
authors.initials: RA
authors.lastname: Swan
authors.forename: J L
authors.initials: JL
authors.lastname: Heeney
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D000835:Animals, Wild; D001483:Base Sequence; D015153:Blotting, Western; D004279:DNA, Viral; D015480:Deltaretrovirus Antibodies / Q000097:blood; D015485:Deltaretrovirus Antigens / Q000276:immunology; D006800:Deltaretrovirus Infections / Q000097:blood / Q000276:immunology / Q000662:veterinary* / Q000821:virology; D004797:Enzyme-Linked Immunosorbent Assay; D015686:Gene Products, env / Q000276:immunology; D015683:Gene Products, gag / Q000276:immunology; D016370:Gene Products, rex / Q000235:genetics; D016356:Gene Products, tax / Q000235:genetics; D006801:Humans; D007214:Indonesia; D008969:Molecular Sequence Data; D008992:Monkey Diseases / Q000097:blood / Q000276:immunology / Q000821:virology*; D010802:Phylogeny; D011148:Pongo pygmaeus / Q000097:blood / Q000276:immunology / Q000821:virology*; D015682:Retroviridae Proteins, Oncogenic / Q000276:immunology; D017386:Sequence Homology, Amino Acid; D015303:Simian T-lymphotropic virus 1 / Q000145:classification / Q000235:genetics* / Q000276:immunology*; D054299:env Gene Products, Human Immunodeficiency Virus; D054301:gag Gene Products, Human Immunodeficiency Virus
chemical_list: D004279:DNA, Viral; D015480:Deltaretrovirus Antibodies; D015485:Deltaretrovirus Antigens; D015686:Gene Products, env; D015683:Gene Products, gag; D016370:Gene Products, rex; D016356:Gene Products, tax; D015682:Retroviridae Proteins, Oncogenic; D054299:env Gene Products, Human Immunodeficiency Virus; D054301:gag Gene Products, Human Immunodeficiency Virus; C055265:gp21 protein, Human T-lymphotropic virus 1; C055266:gp46 protein, Human T-cell leukemia virus type I; C060955:p19 protein, Human T-lymphotropic virus 1; C060954:p24 protein, Human T-lymphotropic virus 1