Analysis of cell kinetics after irradiation by flow cytometry: proliferative ability of G2-blocked cells after cell sorting.
Irradiation, dose-dependently, increases the percentage of cells in the G2 + M phase and the duration of cell cycle arrest, reflected by changes in cell kinetics in the first 48 h. Cells in the G2 + M phase are considered radiosensitive but little is known about their proliferative ability. We studied proliferative ability of irradiated G2 phase-arrested cells following flow cytometric cell sorting using fluorescent Hoechst 33342 staining. Although proliferative ability of non-irradiated cells was not different between G1 and G2 + M phases, cells arrested in G2 + M after irradiation, especially at higher doses, showed less ability than cells in G1. Proliferative ability also correlated well with of G2 block duration.
Murata
T
T
Department of Radiology, Kansai Medical University, 10-15 Fumizonochou, Moriguchi City, Osaka, 570, Japan.
Akagi
K
K
Kimura
H
H
Nasu
R
R
Imamura
M
M
Tanaka
Y
Y
D001562:Benzimidazoles / Q000633:toxicity; D002455:Cell Division / Q000502:physiology* / Q000528:radiation effects; D002470:Cell Survival / Q000528:radiation effects; D004273:DNA, Neoplasm / Q000032:analysis; D005434:Flow Cytometry; D005456:Fluorescent Dyes / Q000633:toxicity; D016193:G1 Phase / Q000502:physiology / Q000528:radiation effects; D016195:G2 Phase / Q000502:physiology* / Q000528:radiation effects; D005720:Gamma Rays; D006367:HeLa Cells / Q000187:drug effects / Q000528:radiation effects; D006801:Humans; D007700:Kinetics; D008938:Mitosis / Q000502:physiology / Q000528:radiation effects; D011003:Ploidies
D001562:Benzimidazoles; D004273:DNA, Neoplasm; D005456:Fluorescent Dyes; C017807:bisbenzimide ethoxide trihydrochloride