Oligonucleotide ligation assay for detecting mutations in the human immunodeficiency virus type 1 pol gene that are associated with resistance to zidovudine, didanosine, and lamivudine.
This report describes the detection of mutations in the pol gene of human immunodeficiency virus type 1 associated with resistance to zidovudine, didanosine, and lamivudine by genotyping by an oligonucleotide ligation assay specific codons in the pol gene amplified by PCR. Our studies demonstrate the sensitivity, simplicity, and specificity of this genotyping system.
Edelstein
R E
RE
Department of Pediatrics, University of Washington, Seattle, USA.
Nickerson
D A
DA
Tobe
V O
VO
Manns-Arcuino
L A
LA
Frenkel
L M
LM
D019380:Anti-HIV Agents / Q000627:therapeutic use*; D003062:Codon; D016049:Didanosine / Q000627:therapeutic use; D004352:Drug Resistance, Microbial / Q000235:genetics*; D015751:Genes, pol; D015658:HIV Infections / Q000188:drug therapy* / Q000235:genetics; D015497:HIV-1 / Q000187:drug effects / Q000235:genetics*; D006801:Humans; D019259:Lamivudine / Q000627:therapeutic use; D009154:Mutation; D015345:Oligonucleotide Probes / Q000235:genetics; D016133:Polymerase Chain Reaction / Q000379:methods; D012367:RNA, Viral / Q000032:analysis / Q000235:genetics; D012680:Sensitivity and Specificity; D017422:Sequence Analysis, DNA; D015215:Zidovudine / Q000627:therapeutic use
D019380:Anti-HIV Agents; D003062:Codon; D015345:Oligonucleotide Probes; D012367:RNA, Viral; D019259:Lamivudine; D015215:Zidovudine; D016049:Didanosine