GM-CSF production by epithelial cell line: upregulation by ultraviolet A.
It was demonstrated that UVB increases synthesis and expression of IL-1 alpha and GM-CSF by keratinocytes. Upregulation of GM-CSF by UVB is reported to be mediated by IL-1 alpha. However, regulation of IL-1 alpha and GM-CSF by UVA is not well-known. The purpose of the present study was to evaluate the effects of UVA on IL-1 alpha and GM-CSF production. Here we used a competitive RT-PCR for measuring cytokine gene expression in an epidermal cell line after UVA irradiation. IL-1 alpha and GM-CSF mRNA did not show any change at 1 h and 6 h following exposure to UVA. After UVA irradiation, however, IL-1 alpha mRNA decreased and GM-CSF mRNA increased at 24 h and the level of GM-CSF in culture supernatant increased at 24 h and 48 h. Addition of antihuman IL-1 alpha neutralizing antibody to UVA irradiated cells did not prevent the increase of GM-CSF mRNA expression. These results suggest that UVA radiation may induce GM-CSF production through an IL-1 alpha independent pathway.
Park
K C
KC
Department of Dermatology, Seoul National University College of Medicine, Korea.
Jung
H C
HC
Hwang
J H
JH
Youn
S W
SW
Ahn
J S
JS
Park
S B
SB
Kim
K H
KH
Chung
J H
JH
Youn
J I
JI
D000906:Antibodies; D002460:Cell Line; D002470:Cell Survival / Q000528:radiation effects; D017931:DNA Primers; D004797:Enzyme-Linked Immunosorbent Assay; D000078404:Epidermal Cells; D005786:Gene Expression Regulation / Q000528:radiation effects; D016178:Granulocyte-Macrophage Colony-Stimulating Factor / Q000096:biosynthesis / Q000235:genetics / Q000528:radiation effects*; D006801:Humans; D007375:Interleukin-1 / Q000096:biosynthesis / Q000235:genetics / Q000276:immunology / Q000528:radiation effects; D015603:Keratinocytes / Q000378:metabolism / Q000528:radiation effects*; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000235:genetics / Q000528:radiation effects; D013997:Time Factors; D014158:Transcription, Genetic; D014466:Ultraviolet Rays / Q000145:classification*; D015854:Up-Regulation / Q000528:radiation effects*
D000906:Antibodies; D017931:DNA Primers; D007375:Interleukin-1; D012333:RNA, Messenger; D016178:Granulocyte-Macrophage Colony-Stimulating Factor