Rhamnogalacturonan I from xylem differentiating zones of Cryptomeria japonica.
Rhamnogalacturonan I (RG-I) was isolated from the pectin of the cell wall of xylem differentiating zones of sugi (Cryptomeria japonica D. Don, gymnosperm) by digesting with endo-alpha-1,4-polygalacturonase followed by gel-permeation chromatography. Structure of RG-I was characterized by glycosyl composition and glycosyl linkage analyses, partial acid hydrolysis, and lithium (Li)-treatment in ethylenediamine. The oligosaccharides produced by partial acid hydrolysis showed the backbone of RG-I to be a disaccharide repeating unit of --> 4)-alpha-D-GalpA-(1-->2)-alpha-L-Rhap-(1-->. Arabian and oligogalactosyl side chains were attached at O-4 of rhamnosyl residues of the backbone. Glycosyl composition and glycosyl linkage analyses suggested that the structures and/or the distribution of the arabinan sidechains of sugi RG-I were different from those of sycamore (angiosperm) RG-I.
Edashige
Y
Y
Forestry and Forest Products Research Institute, Ibaraki, Japan.
Ishii
T
T
D002236:Carbohydrate Conformation; D002240:Carbohydrate Sequence; D002473:Cell Wall / Q000737:chemistry; D002850:Chromatography, Gel; D032462:Cycadopsida / Q000166:cytology / Q000502:physiology*; D008969:Molecular Sequence Data; D009005:Monosaccharides / Q000032:analysis; D010368:Pectins / Q000096:biosynthesis / Q000737:chemistry* / Q000302:isolation & purification; D016339:Spectrometry, Mass, Fast Atom Bombardment; D018629:Spectrometry, Mass, Secondary Ion
D009005:Monosaccharides; C042491:rhamnogalacturonan I; D010368:Pectins