The Anopheles albimanus white gene: molecular characterization of the gene and a spontaneous white gene mutation.
We have cloned and characterized the white gene of Anopheles albimanus. Comparison of the deduced amino acid sequence of this white gene with its homologs from six species of Diptera show that the An. albimanus gene is most similar to the white gene of An. gambiae (92% identity). A spontaneous white-eyed mutant An. albimanus was caused by an approximately 10 kb insertion into a CT dinucleotide repeat region of intron 2 of the white locus. The flanks of this insertion are long (at least 400 bp), nearly perfect inverted terminal repeat sequences. This cloned white gene should be useful as a marker for germ line transformation of An. albimanus.
Ke
Z
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Division of Parasitic Diseases, Centers for Disease Control and Prevention, Chamblee, GA 30341, USA.
Benedict
M Q
MQ
Cornel
A J
AJ
Besansky
N J
NJ
Collins
F H
FH
D018528:ATP-Binding Cassette Transporters; D000595:Amino Acid Sequence; D000818:Animals; D000852:Anopheles / Q000235:genetics*; D001483:Base Sequence; D003001:Cloning, Molecular; D017931:DNA Primers; D029721:Drosophila Proteins; D005136:Eye Proteins / Q000096:biosynthesis / Q000737:chemistry / Q000235:genetics*; D017344:Genes, Insect; D007438:Introns; D007814:Larva; D008969:Molecular Sequence Data; D009154:Mutation; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000737:chemistry; D015183:Restriction Mapping; D012469:Salivary Glands / Q000166:cytology; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid
D018528:ATP-Binding Cassette Transporters; D017931:DNA Primers; D029721:Drosophila Proteins; D005136:Eye Proteins; D011994:Recombinant Proteins; C059254:w protein, Drosophila