Expression and regulation of galanin-R2 receptors in rat primary sensory neurons: effect of axotomy and inflammation.
Using in situ hybridization, we studied galanin-R2 receptor (GAL-R2-R) mRNA in rat lumbar 5 dorsal root ganglia (DRGs) at different time points after peripheral tissue inflammation and sciatic nerve transection (axotomy). About 25% of all normal DRG neuron profiles were GAL-R2-R mRNA-positive, and the majority was of the small type. In normal DRGs GAL-R2-R mRNA often (approximately 80%) colocalized with CGRP mRNA and sometimes (approximately 20%) with GAL-R1-R mRNA. There was a strong increase in the number and labeling intensity of GAL-R2-R mRNA-positive neuron profiles after peripheral tissue inflammation with a peak at 3 days, as well as a long-lasting decrease after axotomy. These results, together with the previously shown regulation of GAL and the GAL-R1-R, suggest that GALergic mechanisms participate in complex adaptive responses in DRGs after inflammation and nerve injury.
Sten Shi
T J
TJ
Department of Neuroscience, Karolinska Institutet, Stockholm, Sweden.
Zhang
X
X
Holmberg
K
K
Xu
Z Q
ZQ
Hökfelt
T
T
D000818:Animals; D019771:Axotomy; D015740:Calcitonin Gene-Related Peptide / Q000096:biosynthesis; D019204:GTP-Binding Proteins / Q000096:biosynthesis* / Q000378:metabolism*; D005727:Ganglia, Spinal / Q000166:cytology / Q000378:metabolism; D017403:In Situ Hybridization; D009443:Neuritis / Q000378:metabolism*; D009475:Neurons, Afferent / Q000378:metabolism*; D015345:Oligonucleotide Probes; D012333:RNA, Messenger / Q000096:biosynthesis; D051381:Rats; D017207:Rats, Sprague-Dawley; D044088:Receptors, Galanin; D011964:Receptors, Gastrointestinal Hormone / Q000096:biosynthesis* / Q000378:metabolism*
D015345:Oligonucleotide Probes; D012333:RNA, Messenger; D044088:Receptors, Galanin; D011964:Receptors, Gastrointestinal Hormone; D019204:GTP-Binding Proteins; D015740:Calcitonin Gene-Related Peptide