Influence of aqueous extracts of medicinal plants on surface hydrophobicity of Escherichia coli strains of different origin.
The adhesion of microbes on host cells is of decisive importance in the development of Gram-negative microbe-induced infections and can be influenced by the surface hydrophobicity of the microbial cell. The hydrophobicity of 155 Escherichia coli strains of different origin was determined by the salt aggregation test (SAT). Among the strains isolated from faecal samples of healthy persons only 16.7% showed aggregative properties, whereas among the strains isolated from the urine of patients with pyelonephritis and the faecal samples of calves and pigs with diarrhoea some 40.0%-60.0% were aggregative. The influence of aqueous extracts prepared from bearberry leaves, St. John's wort herbs, wild camomile and marigold flowers on hydrophobicity of 40 E. coli and 20 Acinetobacter baumannii strains was investigated. The decoctions of bearberry and St. John's wort increased remarkably the hydrophobicity of both microbial species. The infusions of wild camomile and marigold completely blocked the aggregative properties of the investigated strains. Bactericidal action was relatively low in the case of bearberry and St. John's wort and completely lacking in the case of wild camomile and marigold. Thus, one of the probable and potentially important action mechanisms of the four medicinal plants studied is their ability to influence the surface characteristics of the microbial cells and thereby their putative virulence properties.
Türi
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M
Department of Microbiology, University of Tartu, Estonia.
Türi
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Kõljalg
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Mikelsaar
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M
D000818:Animals; D001422:Bacterial Adhesion; D002417:Cattle; D004926:Escherichia coli / Q000737:chemistry*; D004927:Escherichia coli Infections / Q000382:microbiology*; D006801:Humans; D019684:Magnoliopsida; D010936:Plant Extracts / Q000494:pharmacology*; D010946:Plants, Medicinal / Q000737:chemistry*; D012995:Solubility; D013499:Surface Properties; D013552:Swine
D010936:Plant Extracts