title: Follicular fluid hormone concentrations during controlled ovarian hyperstimulation using gonadotropin preparations with different FSH/LH ratios. I. Comparison of an FSH-dominant and a purified FSH preparation.
abstract: OBJECTIVE
  The aim of the present randomized study was to investigate whether ovarian stimulation treatment with gonadotropin preparations containing different amounts of LH activity resulted in variations of steroidal composition of follicular fluid. A different endocrine milieu within the follicle might influence oocyte quality.
  
  
  METHODS
  Antral fluid from mature follicles was collected in in vitro fertilization cycles and concentrations of testosterone, androstenedione, estrone, estradiol, progesterone, FSH, and LH were determined. A comparison was made between treatment with a purified FSH preparation (nine patients, 35 follicular fluid samples) and a FSH-dominant human menopausal gonadotropin (hMG) preparation (nine patients, 34 samples).
  
  
  RESULTS
  No differences in any of the hormone levels could be detected between the two groups.
  
  
  CONCLUSIONS
  Treatment with gonadotropin preparations containing different FSH/LH ratios did not result in different androgen, estrogen and progesterone levels in follicular fluid.
authors.affiliation: Department of Obstetrics and Gynecology, University Hospital Nijmegen St. Radboud, The Netherlands.
authors.forename: I J
authors.initials: IJ
authors.lastname: Duijkers
authors.forename: W N
authors.initials: WN
authors.lastname: Willemsen
authors.forename: H M
authors.initials: HM
authors.lastname: Hollanders
authors.forename: C J
authors.initials: CJ
authors.lastname: Hamilton
authors.forename: C M
authors.initials: CM
authors.lastname: Thomas
authors.forename: H M
authors.initials: HM
authors.lastname: Vemer
mesh_terms: D000328:Adult; D015331:Cohort Studies; D004338:Drug Combinations; D005260:Female; D005307:Fertilization in Vitro / Q000379:methods*; D005640:Follicle Stimulating Hormone / Q000008:administration & dosage / Q000032:analysis / Q000494:pharmacology; D015571:Follicular Fluid / Q000737:chemistry* / Q000187:drug effects; D012739:Gonadal Steroid Hormones / Q000032:analysis* / Q000145:classification; D006065:Gonadotropins, Pituitary / Q000008:administration & dosage* / Q000032:analysis / Q000145:classification / Q000494:pharmacology; D006801:Humans; D007986:Luteinizing Hormone / Q000008:administration & dosage / Q000032:analysis / Q000494:pharmacology; D010062:Ovulation Induction / Q000009:adverse effects / Q000379:methods*
chemical_list: D004338:Drug Combinations; D012739:Gonadal Steroid Hormones; D006065:Gonadotropins, Pituitary; D007986:Luteinizing Hormone; D005640:Follicle Stimulating Hormone