title: Cytotoxic T-lymphocyte responses in cattle infected with bovine viral diarrhea virus.
abstract: A system for a reproducible in vitro restimulation of bovine viral diarrhea virus (BVDV)-specific cytotoxic T-cells (CTL) was developed. Lymphocyte cultures of BVDV-immunised cattle were stimulated with infectious BVDV isolate PT810 and recombinant bovine interleukin-2 for 12 to 25 days. A specific lysis of Concanavalin A-stimulated BVDV-infected autologous target cells was observed, whereas allogeneic BVDV-infected target cells were only marginally lysed as detected by flow cytometry. BVDV-specific lymphocyte transformation was further characterised by the expression of bovine lymphocyte activation antigens and bovine MHC class-II molecules. Secondary stimulation of CTL was influenced by in vitro production of BVDV-specific neutralising antibodies, which were secreted exclusively in BVDV-inoculated lymphocyte cultures of immunised cattle. These results demonstrate the presence of CTL in peripheral blood mononuclear cells (PBMC) of immunised cattle which can kill autologous BVDV-infected antigen-presenting cells after in vitro restimulation.
authors.affiliation: Institute for Medical Microbiology, Infectious and Epidemic Diseases, Ludwig-Maximilians-University Munich, Germany. martin.beer@Irz.uni-muenchen.de
authors.forename: M
authors.initials: M
authors.lastname: Beer
authors.forename: G
authors.initials: G
authors.lastname: Wolf
authors.forename: J
authors.initials: J
authors.lastname: Pichler
authors.forename: A
authors.initials: A
authors.lastname: Wolfmeyer
authors.forename: O R
authors.initials: OR
authors.lastname: Kaaden
mesh_terms: D000818:Animals; D000914:Antibodies, Viral / Q000032:analysis; D001912:Bovine Virus Diarrhea-Mucosal Disease / Q000097:blood / Q000276:immunology* / Q000473:pathology; D002417:Cattle; D003601:Cytotoxicity Tests, Immunologic / Q000662:veterinary; D001908:Diarrhea Viruses, Bovine Viral / Q000276:immunology*; D005434:Flow Cytometry / Q000662:veterinary; D019084:Fluorescent Antibody Technique, Indirect / Q000662:veterinary; D000949:Histocompatibility Antigens Class II / Q000097:blood / Q000276:immunology*; D007111:Immunity, Cellular; D007376:Interleukin-2 / Q000276:immunology; D008213:Lymphocyte Activation; D009500:Neutralization Tests / Q000662:veterinary; D011994:Recombinant Proteins / Q000276:immunology; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology* / Q000821:virology*; D014765:Viral Vaccines / Q000276:immunology
chemical_list: D000914:Antibodies, Viral; D000949:Histocompatibility Antigens Class II; D007376:Interleukin-2; D011994:Recombinant Proteins; D014765:Viral Vaccines