GnRH perikarya in medial basal hypothalamus of pubertal female rhesus macaque are ensheathed with glia.
A previous study in our laboratory revealed that the cell bodies of gonadotropin releasing hormone (GnRH) neurons in the preoptic area (POA) of early to midpubertal female rhesus monkeys were extensively invested with thick glial processes. Because the medial basal hypothalamus (MBH) plays a critical role in the control of pulsatile and cyclic gonadotropin release in the primate, we have now focused on the ultrastructural milieu of GnRH neurons of this region in the same sample of monkeys. The ensheathment of the perikarya of GnRH neurons in the MBH with such glial processes was more pronounced than in the POA. Whereas the mean proportion of the cell membrane covered by these glia was 57% in the POA, it was 72% in the MBH. In addition, the cell bodies of GnRH neurons in the MBH of the pubertal monkey (unlike those in the POA) were less well innervated than were those in the adult cycling monkey, further highlighting differences between these brain regions. Differences in the anatomical milieu of the MBH between immature monkeys, in which GnRH release is still relatively quiescent, and adult cycling monkeys are consistent with the hypothesis that GnRH neurons within the MBH are under particular constraint in the immature animals. The functional significance of these observations must, however, await further studies.
Witkin
J W
JW
Department of Anatomy and Cell Biology, Columbia University College of Physicians and Surgeons, New York, NY 10032, USA.
O'Sullivan
H
H
Miller
R
R
Ferin
M
M
D000818:Animals; D005260:Female; D007987:Gonadotropin-Releasing Hormone / Q000502:physiology*; D007033:Hypothalamus, Middle / Q000254:growth & development*; D008253:Macaca mulatta; D009457:Neuroglia / Q000502:physiology*; D009474:Neurons / Q000502:physiology*; D011301:Preoptic Area / Q000254:growth & development
D007987:Gonadotropin-Releasing Hormone