title: Seroreactive species-specific lipooligosaccharides of Mycobacterium mucogenicum sp. nov. (formerly Mycobacterium chelonae-like organisms): identification and chemical characterization.
abstract: Strains of the new species Mycobacterium mucogenicum exhibit physiological and biochemical features very similar to those of the other species of the Mycobacterium fortuitum complex. To define taxonomic criteria for easy identification of M. mucogenicum, the glycolipid patterns of the reference strains and of 32 environmental and clinical isolates were examined by TLC. It was concluded that all M. mucogenicum strains of smooth colony morphology contained species-specific alkali-labile glycoconjugates. Three different patterns were observed among the strains of the smooth colony type. Fractionation followed by conventional chemical analyses of the purified glycolipids showed the specific glycolipids to be lipooligosaccharides (LOS). The three LOS showed a similar fatty acid composition consisting of straight chain (dodeca-, tetradeca-, hexadecanoyl and hexadecenoyl) and methylbranched (2,4-dimethyleicosanoyl and 2,4-dimethyleicosenoyl) fatty acyl substituents. The most commonly encountered LOS (present in 76% of the smooth strains) contained a tetraacylated pentasaccharide composed of four moles of glucose and one mole of a 2,4-di-O-methylhexose. A LOS composed of arabinose, glucose and mannose was present in 20% of the smooth strains, whereas the newly proposed type strain of M. mucogenicum (ATCC 49650) was the only strain that contained a LOS composed of glucose and galactose. Serological studies clearly differentiated most of the strains of M. mucogenicum from those of the other members of the M. fortuitum complex, and demonstrated the existence of serovars within the former species. Altogether, these data confirm the validity of the new species but show ATCC 49651 to be the most representative strain.
authors.affiliation: Institut de Pharmacologie et de Biologie Structurale du CNRS, 205 route de Narbonne, 31077 Toulouse cedex, France.|Departamento de Genética y de Microbiología, Unidad de Microbiología, Facultad de Ciencias, Universidad Autónoma de Barcelona, 08193 Bellaterra (Barcelona), Spain.
authors.forename: Manuel
authors.initials: M
authors.lastname: Muñoz
authors.affiliation: Institut de Pharmacologie et de Biologie Structurale du CNRS, 205 route de Narbonne, 31077 Toulouse cedex, France.
authors.forename: Catherine
authors.initials: C
authors.lastname: Raynaud
authors.affiliation: Institut de Pharmacologie et de Biologie Structurale du CNRS, 205 route de Narbonne, 31077 Toulouse cedex, France.
authors.forename: Marie-Antoinette
authors.initials: MA
authors.lastname: Lanéelle
authors.affiliation: Departamento de Genética y de Microbiología, Unidad de Microbiología, Facultad de Ciencias, Universidad Autónoma de Barcelona, 08193 Bellaterra (Barcelona), Spain.
authors.forename: Esther
authors.initials: E
authors.lastname: Julián
authors.affiliation: Institut de Pharmacologie et de Biologie Structurale du CNRS, 205 route de Narbonne, 31077 Toulouse cedex, France.
authors.forename: Luz María López
authors.initials: LML
authors.lastname: Marín
authors.affiliation: Institut de Pharmacologie et de Biologie Structurale du CNRS, 205 route de Narbonne, 31077 Toulouse cedex, France.
authors.forename: Gaby
authors.initials: G
authors.lastname: Silve
authors.affiliation: Servicio de Microbiología, Hospital Universitario Germans Trias i Pujol, 08916 Badalona, (Barcelona), Spain.
authors.forename: Vicente
authors.initials: V
authors.lastname: Ausina
authors.affiliation: Institut de Pharmacologie et de Biologie Structurale du CNRS, 205 route de Narbonne, 31077 Toulouse cedex, France.
authors.forename: Mamadou
authors.initials: M
authors.lastname: Daffé
authors.affiliation: Departamento de Genética y de Microbiología, Unidad de Microbiología, Facultad de Ciencias, Universidad Autónoma de Barcelona, 08193 Bellaterra (Barcelona), Spain.
authors.forename: Marina
authors.initials: M
authors.lastname: Luquin
mesh_terms: D000818:Animals; D006017:Glycolipids / Q000032:analysis* / Q000737:chemistry; D006801:Humans; D009161:Mycobacterium / Q000145:classification* / Q000166:cytology / Q000502:physiology; D009164:Mycobacterium Infections / Q000382:microbiology; D016926:Mycobacterium chelonae / Q000145:classification / Q000166:cytology / Q000472:pathogenicity; D011817:Rabbits; D012703:Serotyping
chemical_list: D006017:Glycolipids