Capillary electrophoresis STR analysis: comparison to gel-based systems.
Capillary electrophoresis is a relatively new technology for the forensic analysis of STRs, and may be an excellent alternative to traditional gel-based methods. Eighty samples, obtained from six different laboratories which had previously analyzed the samples using a variety of gel-based methods, were reanalyzed by CE. It was determined that CE could obtain the same allelic designation as that reported by the comparison laboratory. Seven different loci employing two separate multiplex amplifications were used for the comparison study. One basepair resolution and average standard deviations within the range of 0.075 to 0.117 basepairs were observed.
Buel
E
E
State of Vermont Forensic Laboratory, Waterbury, USA.
Schwartz
M B
MB
LaFountain
M J
MJ
D000483:Alleles; D001482:Base Composition; D003020:Clothing; D004247:DNA / Q000032:analysis* / Q000097:blood / Q000235:genetics; D019075:Electrophoresis, Capillary / Q000379:methods*; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods*; D005554:Forensic Medicine / Q000379:methods*; D005838:Genotype; D006801:Humans; D010209:Paper; D012091:Repetitive Sequences, Nucleic Acid / Q000235:genetics*
D004247:DNA