[Effect of HPA-1a(PLA1) and HLA antibodies on platelet function, measured with the in vitro bleeding test].
The influence of platelet-specific and HLA antibodies on primary hemostasis was investigated by the in vitro bleeding test (IVBT). Seven of 12 plasmas with HPA-1a (PLA1) antibodies and two with antibodies against glycoprotein Ib/IX significantly inhibited the IVBT of normal cross-match-positive donor blood. This correlated with an increase of GMP-140 on the platelets determined by flow cytometry. Therefore the inhibition of the IVBT was interpreted to be due to rapid platelet activation by platelet-specific antibodies followed by an irreversible loss of function. In contrast, with HLA antibodies neither activation nor inhibition of the platelet function could be proven.
Kretschmer
V
V
Abteilung für Transfusionsmedizin und Gerinnungsphysiologie, Universitätsklinikum, Marburg.
Huss
B
B
Feller
K
K
Kiefel
V
V
D016824:Antigens, Human Platelet / Q000276:immunology*; D001792:Blood Platelets / Q000276:immunology / Q000502:physiology*; D006680:HLA Antigens / Q000276:immunology*; D006487:Hemostasis; D006650:Histocompatibility Testing; D006801:Humans; D007231:Infant, Newborn; D039661:Integrin beta3; D019007:P-Selectin / Q000502:physiology; D010979:Platelet Function Tests / Q000379:methods; D019038:Platelet Glycoprotein GPIb-IX Complex / Q000502:physiology; D013921:Thrombocytopenia / Q000097:blood* / Q000276:immunology
D016824:Antigens, Human Platelet; D006680:HLA Antigens; C061248:ITGB3 protein, human; D039661:Integrin beta3; D019007:P-Selectin; D019038:Platelet Glycoprotein GPIb-IX Complex
Einfluss von HPA-1a(PLA1)- und HLA-Antikörpern auf die Plättchenfunktion, gemessen mit dem In-vitro-Blutungstest.