Altered [Ca2+] homeostasis in PC12 cells after nerve growth factor deprivation.
[Ca2+]i homeostasis in individual PC12 cells after elevated [K+]o was studied by ratiometric microscopy, during nerve growth factor (NGF) deprivation. A significantly lower number of cells responded with an increased [Ca2+]i in the NGF deprived condition. Moreover, the responding cells were more deficient in regulating their [Ca2+]i back to control levels, after the transient peak. This suggests that differentiated neurons do not traverse the apoptotic program homogeneously with regard to their [Ca2+]i regulation and that NGF deprived PC12 cells have more difficulties to reduce their [Ca2+]i after influx of [Ca2+]o.
Dispersyn
G
G
Department of Biochemistry, University of Antwerp, Belgium.
Nuydens
R
R
de Jong
M
M
Borgers
M
M
Geerts
H
H
D000818:Animals; D015220:Calcium Channels / Q000502:physiology*; D006706:Homeostasis; D016014:Linear Models; D008564:Membrane Potentials / Q000502:physiology; D009414:Nerve Growth Factors / Q000172:deficiency*; D009474:Neurons / Q000502:physiology*; D016716:PC12 Cells; D011188:Potassium / Q000502:physiology*; D051381:Rats
D015220:Calcium Channels; D009414:Nerve Growth Factors; D011188:Potassium