Metabotropic glutamate receptor agonists stimulate polyphosphoinositide hydrolysis in primary cultures of rat hepatocytes.
The metabotropic glutamate (mGlu) receptor agonists, quisqualate and (1S,3R)-1-aminocyclopentane-1,3-dicarboxylic acid (ACPD), but not (RS)-3,5-dihydroxyphenylglycine or (2S,3S,4S)-alpha-(carboxycyclopropyl)glycine, stimulated [3H]inositolmonophosphate ([3H]InsP) formation in primary cultures of rat hepatocytes. 1S,3R-ACPD-stimulated [3H]InsP formation was inhibited by alpha-methyl-4-carboxyphenylglycine, indicating that cultured hepatocytes express functional mGlu receptors coupled to polyphosphoinositide hydrolysis. The identity of these receptors is not similar to that of any of the known mGlu receptor subtypes characterized in heterologous expression systems.
Sureda
F
F
I.M.N., Pozzilli, Italy.
Copani
A
A
Bruno
V
V
Knöpfel
T
T
Meltzger
G
G
Nicoletti
F
F
D000818:Animals; D002478:Cells, Cultured; D003515:Cycloleucine / Q000031:analogs & derivatives / Q000494:pharmacology; D018690:Excitatory Amino Acid Agonists / Q000494:pharmacology; D008099:Liver / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D018696:Neuroprotective Agents / Q000494:pharmacology; D018129:Phosphatidylinositol Phosphates / Q000378:metabolism*; D016318:Quisqualic Acid / Q000494:pharmacology; D051381:Rats; D018094:Receptors, Metabotropic Glutamate / Q000819:agonists*
D018690:Excitatory Amino Acid Agonists; D018696:Neuroprotective Agents; D018129:Phosphatidylinositol Phosphates; D018094:Receptors, Metabotropic Glutamate; D003515:Cycloleucine; C036361:1-amino-1,3-dicarboxycyclopentane; D016318:Quisqualic Acid