title: Accumulation of human immunodeficiency virus-specific cytotoxic T lymphocytes away from the predominant site of virus replication during primary infection.
abstract: Down-regulation of the initial burst of viremia during primary human immunodeficiency virus (HIV) infection is thought to be mediated predominantly by HIV-specific CD8+ cytotoxic T lymphocytes (CTL). This response is associated with major perturbations in the T cell receptor (TCR) repertoire. To investigate the failure of the cellular immune response to adequately control viral spread and replication and to prevent establishment of HIV infection, changes in the TCR repertoire and in the distribution of virus-specific CTL between blood and lymph node were analyzed in three patients with primary infection. By the combined use of clonotype-specific polymerase chain reaction and analysis of the frequency of in vivo activated HIV-specific CTL, it was shown that HIV-specific CTL clones preferentially accumulated in blood as opposed to lymph node. Accumulation of HIV-specific CTL in blood occurred prior to effective down-regulation of virus replication in both blood and lymph node. These findings should provide new insights into how HIV, and possibly other viruses, elude the immune response of the host during primary infection.
authors.affiliation: Department of Medicine, Centre Hospitalier Universitaire Vaudois, Lausanne, Switzerland. Guiseppe.Pantaleo@chuv.hospvd.ch
authors.forename: G
authors.initials: G
authors.lastname: Pantaleo
authors.forename: H
authors.initials: H
authors.lastname: Soudeyns
authors.forename: J F
authors.initials: JF
authors.lastname: Demarest
authors.forename: M
authors.initials: M
authors.lastname: Vaccarezza
authors.forename: C
authors.initials: C
authors.lastname: Graziosi
authors.forename: S
authors.initials: S
authors.lastname: Paolucci
authors.forename: M B
authors.initials: MB
authors.lastname: Daucher
authors.forename: O J
authors.initials: OJ
authors.lastname: Cohen
authors.forename: F
authors.initials: F
authors.lastname: Denis
authors.forename: W E
authors.initials: WE
authors.lastname: Biddison
authors.forename: R P
authors.initials: RP
authors.lastname: Sekaly
authors.forename: A S
authors.initials: AS
authors.lastname: Fauci
mesh_terms: D017951:Antigen Presentation / Q000276:immunology; D000956:Antigens, Viral / Q000276:immunology; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology* / Q000473:pathology; D002465:Cell Movement / Q000276:immunology*; D015658:HIV Infections / Q000276:immunology*; D015497:HIV-1 / Q000502:physiology*; D006801:Humans; D008198:Lymph Nodes / Q000276:immunology / Q000473:pathology; D016693:Receptors, Antigen, T-Cell, alpha-beta / Q000276:immunology*; D016176:T-Lymphocyte Subsets / Q000276:immunology* / Q000473:pathology; D014779:Virus Replication / Q000276:immunology
chemical_list: D000956:Antigens, Viral; D016693:Receptors, Antigen, T-Cell, alpha-beta