title: Matrix metalloproteinase inhibitors: a structure-activity study.
abstract: Modifications around the dipeptide-mimetic core of a hydroxamic acid based matrix metalloproteinase inhibitor were studied. These variations incorporated a variety of natural, unnatural, and synthetic amino acids in addition to modifications of the P1' and P3' substituents. The results of this study indicate the following structural requirements: (1) Two key hydrogen bonds must be present between the enzyme and potent substrates. (2) Potent inhibitors must possess strong zinc-binding functionalities. (3) The potential importance of the hydrophobic group at position R3 as illustrated by its ability to impart greater relative potency against stromelysin when larger hydrophobic groups are used. (4) Requirements surrounding the nature of the amino acid appear to be more restrictive for stromelysin than for neutrophil collagenase, 72 kDa gelatinase, and 92 kDa gelatinase. These requirements may involve planar fused-ring aryl systems and possibly hydrogen-bonding capabilities.
authors.affiliation: Department of Chemistry, Glycomed, Inc., Alameda, California 94501, USA.
authors.forename: D E
authors.initials: DE
authors.lastname: Levy
authors.forename: F
authors.initials: F
authors.lastname: Lapierre
authors.forename: W
authors.initials: W
authors.lastname: Liang
authors.forename: W
authors.initials: W
authors.lastname: Ye
authors.forename: C W
authors.initials: CW
authors.lastname: Lange
authors.forename: X
authors.initials: X
authors.lastname: Li
authors.forename: D
authors.initials: D
authors.lastname: Grobelny
authors.forename: M
authors.initials: M
authors.lastname: Casabonne
authors.forename: D
authors.initials: D
authors.lastname: Tyrrell
authors.forename: K
authors.initials: K
authors.lastname: Holme
authors.forename: A
authors.initials: A
authors.lastname: Nadzan
authors.forename: R E
authors.initials: RE
authors.lastname: Galardy
mesh_terms: D002851:Chromatography, High Pressure Liquid; D004151:Dipeptides / Q000737:chemistry* / Q000494:pharmacology*; D018093:Gelatinases / Q000037:antagonists & inhibitors*; D007700:Kinetics; D020778:Matrix Metalloproteinase 2; D020784:Matrix Metalloproteinase 8; D020780:Matrix Metalloproteinase 9; D061965:Matrix Metalloproteinase Inhibitors; D008666:Metalloendopeptidases / Q000037:antagonists & inhibitors*; D008956:Models, Chemical; D011480:Protease Inhibitors / Q000737:chemistry* / Q000494:pharmacology*; D013329:Structure-Activity Relationship
chemical_list: D004151:Dipeptides; D061965:Matrix Metalloproteinase Inhibitors; C078131:N-(2(R)-2-(hydroxamidocarbonylmethyl)-4-methylpentanoyl)-L-tryptophan methylamide; D011480:Protease Inhibitors; D018093:Gelatinases; D008666:Metalloendopeptidases; D020778:Matrix Metalloproteinase 2; D020784:Matrix Metalloproteinase 8; D020780:Matrix Metalloproteinase 9