title: Level of viral antigen in blood leucocytes from cattle acutely infected with bovine viral diarrhoea virus.
abstract: Blood samples from 24 calves undergoing experimental acute infection with a non-cytopathogenic bovine viral diarrhoea virus (BVDV) were examined for viral antigen in peripheral leucocytes with an enzyme-linked immunosorbent assay (ELISA), and for presence of virus in blood plasma in a cell culture assay. With the antigen ELISA, low positive values were detected in leucocytes sampled on days 3-4 from two of eight animals inoculated intranasally, and on days 11-13 from three of 16 animals inoculated intramuscularly. From 22 of the animals, low titres of BVDV were detected in blood plasma obtained 2-9 days after inoculation. All other samples, drawn between 2 and 21 days after inoculation, were negative for viral antigen. All animals seroconverted 3-4 weeks after inoculation, some after having shown mild and transient signs of disease.
authors.affiliation: Department of Virology and Serodiagnostics, Norwegian College of Veterinary Medicine, Oslo, Norway.
authors.forename: T
authors.initials: T
authors.lastname: Sandvik
authors.forename: B
authors.initials: B
authors.lastname: Fredriksen
authors.forename: T
authors.initials: T
authors.lastname: Løken
mesh_terms: D000818:Animals; D000914:Antibodies, Viral / Q000097:blood; D000956:Antigens, Viral / Q000097:blood*; D001912:Bovine Virus Diarrhea-Mucosal Disease / Q000175:diagnosis / Q000276:immunology* / Q000821:virology; D002417:Cattle; D001908:Diarrhea Viruses, Bovine Viral / Q000276:immunology* / Q000302:isolation & purification; D004797:Enzyme-Linked Immunosorbent Assay / Q000662:veterinary; D005260:Female; D007962:Leukocytes / Q000821:virology*
chemical_list: D000914:Antibodies, Viral; D000956:Antigens, Viral