Adenosine-5'-tetraphosphate and guanosine-5'-tetraphosphate: new substrates of the cytosolic exopolyphosphatase of the yeast Saccharomyces cerevisiae.
A cytosolic preparation of Saccharomyces cerevisiae is capable of hydrolyzing adenosine-5'-tetraphosphate and guanosine-5'-tetraphosphate with activities which are 1.5-2 times greater than that with polyP15. The apparent K(m) values for hydrolysis of adenosine-5'-tetraphosphate and guanosine-5'-tetraphosphate are 100 and 80 microM, respectively. A comparative study of inhibitors shows that these activities are inherent characteristics of these exopolyphosphatases.
Kulakovskaya
T V
TV
Skryabin Institute of Biochemistry and Physiology of Microorganisms, Russian Academy of Sciences, Pushchino, Moscow Region, Russia.
Andreeva
N A
NA
Kulaev
I S
IS
D017766:Acid Anhydride Hydrolases / Q000378:metabolism*; D000227:Adenine Nucleotides / Q000378:metabolism*; D003600:Cytosol / Q000201:enzymology; D004791:Enzyme Inhibitors / Q000378:metabolism; D006159:Guanosine Tetraphosphate / Q000378:metabolism*; D006493:Heparin / Q000378:metabolism; D006868:Hydrolysis; D007700:Kinetics; D012441:Saccharomyces cerevisiae / Q000201:enzymology*; D012969:Sodium Fluoride / Q000378:metabolism
D000227:Adenine Nucleotides; D004791:Enzyme Inhibitors; C017991:adenosine 5'-tetraphosphate; D006159:Guanosine Tetraphosphate; D012969:Sodium Fluoride; D006493:Heparin; D017766:Acid Anhydride Hydrolases; C020137:exopolyphosphatase