Difference between blood cells as measured by flow cytofluorometry with a lipophilic fluorescent probe 4-dimethylaminochalcone.
Human peripheral blood was stained with a vital membrane fluorescent probe 4-dimethylaminochalcone (DMC) and studied by flow cytofluorometry. The cell fluorescence histogram contained three parts. A fraction of highly fluorescing cells was attributed to granulocytes, the medium fluorescence intensity was attributed to mononuclears (lymphocytes and monocytes), and weakly fluorescing cells were thrombocytes and erythrocytes. The origin of the fraction completely correlated with the results of the histological count of blood smears. Quantitative analysis of the difference between DMC fluorescence intensities in granulocytes and mononuclear cells suggests that granulocytes may contain intracellular lipoprotein-like particles while in mononuclear cells the lipid forms mainly membrane structures.
Gularyan
S K
SK
Research Institute for Physico-Chemical Medicine, Moscow, Russia.
Svetlichny VYu
Dobretsov
G E
GE
D001773:Blood Cells / Q000737:chemistry*; D002469:Cell Separation; D002599:Chalcone / Q000031:analogs & derivatives*; D047188:Chalcones; D005434:Flow Cytometry / Q000379:methods*; D005456:Fluorescent Dyes; D006801:Humans; D008055:Lipids / Q000097:blood*
D047188:Chalcones; D005456:Fluorescent Dyes; D008055:Lipids; C016396:dimethylaminochalcone; D002599:Chalcone