Relationship between fecal bile acids and the occurrence of colorectal neoplasia in experimental murine ulcerative colitis.
OBJECTIVE
The possible role of fecal bile acids in colorectal carcinogenesis in ulcerative colitis has been reported. In this study, we investigated the relationship between fecal bile acids and the occurrence of colorectal neoplasia in experimental murine colitis induced by dextran sulfate sodium.


METHODS
Colorectal neoplasia in experimental colitis was induced by dextran sulfate sodium subsequent to a single azoxymethane pretreatment. Fecal bile acids were analyzed by gas-liquid chromatography.


RESULTS
Multiple high-grade dysplasias (intramucosal adenocarcinoma) and inflammatory changes were seen in all mice administered dextran sulfate sodium and azoxymethane. Inflammatory changes were also observed in all mice given dextran sulfate sodium only, while neither tumor nor inflammatory changes were detected in any of the control mice. Significant increases in cholic acid were observed in the mice of the colorectal tumor and experimental colitis groups during the experimental period, while in the control mice, no significant changes in fecal bile acids were observed.


CONCLUSIONS
It is suggested that fecal cholic acid and colitis may be intimately related to the development of colorectal neoplasia in this experimental model of murine colitis as well as in ulcerative colitis.
Kajiura
K
K
Second Department of Surgery, Tokyo Medical and Dental University School of Medicine, Japan.
Ohkusa
T
T
Okayasu
I
I
D000230:Adenocarcinoma / Q000139:chemically induced* / Q000378:metabolism / Q000473:pathology; D000818:Animals; D001397:Azoxymethane / Q000633:toxicity*; D001647:Bile Acids and Salts / Q000378:metabolism*; D015197:Carcinogenicity Tests; D002273:Carcinogens / Q000633:toxicity*; D002851:Chromatography, High Pressure Liquid; D003093:Colitis, Ulcerative / Q000150:complications*; D015179:Colorectal Neoplasms / Q000139:chemically induced* / Q000378:metabolism / Q000473:pathology; D016264:Dextran Sulfate / Q000633:toxicity*; D004195:Disease Models, Animal; D051379:Mice; D012720:Severity of Illness Index
D001647:Bile Acids and Salts; D002273:Carcinogens; D016264:Dextran Sulfate; D001397:Azoxymethane