[Effect of various rabbit complement batches of HLA class I antigen determination in lymphocytotoxicity testing].
HLA class I lymphocytotoxicity testing is strongly complement-dependent. We tested 13 commercially available rabbit complement batches (7 distributors) using a panel of 14 donors with decisive patterns of HLA antigens. We defined a scoring system that weights the different possible faulty reactions. The rating score ranged from 12.45 to 46.55 (median 16.09). Comparing the reactions in batches with low and high rating scores gave statistically significant differences (chi 2 test, p < 0.05). Our complement reaction score may be helpful to find the optimal complement batch for HLA class I lymphocytotoxicity testing.
Wittmann
G
G
Blutbank, Abteilung Innere Medizin m.S. Hämatologie und Onkologie, Universitätsklinikum Rudolf Virchow, Berlin.
Zingsem
J
J
Zeiler
T
T
Zimmermann
R
R
Eckstein
R
R
D000818:Animals; D001782:Blood Donors; D003165:Complement System Proteins / Q000276:immunology*; D003602:Cytotoxicity, Immunologic; D015395:Histocompatibility Antigens Class I / Q000276:immunology*; D006650:Histocompatibility Testing; D011817:Rabbits
D015395:Histocompatibility Antigens Class I; D003165:Complement System Proteins
Der Einfluss verschiedener Kaninchenkomplement-Chargen auf die HLA-Klasse-I-Antigenbestimmung im Lymphozytotoxizitätstest.