title: Monokine production following in vitro stimulation of the THP-1 human monocytic cell line with pertussis vaccine components.
abstract: Whole-cell pertussis found in diphtheria-tetanus-pertussis (DTP) vaccine can produce symptoms reminiscent of biological responses to circulating proinflammatory monokines such as IL-6, IL-1beta, and TNFalpha. Therefore the ability of pertussis-containing vaccines and several heat-killed Bordetella pertussis preparations to stimulate cytokine production in a human monocytic cell line, THP-1, were examined. The whole-cell pertussis vaccine induced significantly more IL-6, IL-1beta, and TNFalpha production than did the acellular pertussis or diphtheria-tetanus-only vaccine. Polymyxin B was able to inhibit most of the IL-6 induced by pertussis endotoxin and a heat-killed preparation of B. pertussis containing a null mutation in bvgAS, a regulatory locus required for expression of all known protein virulence factors synthesized by this organism. However, it only partially inhibited IL-6 production induced by other pertussis-containing preparations, including DTP vaccine. These results indicate that in vitro whole-cell vaccine is a potent stimulator of IL-6, IL-1beta, and TNFalpha. They also suggest that although endotoxin is a major inducer of IL-6, other components of B. pertussis also contribute to IL-6 production by monocytes.
authors.affiliation: UCLA School of Nursing, UCLA School of Medicine, Los Angeles, California 90095, USA.
authors.forename: J
authors.initials: J
authors.lastname: Blood-Siegfried
authors.forename: E
authors.initials: E
authors.lastname: Crabb Breen
authors.forename: S
authors.initials: S
authors.lastname: Takeshita
authors.forename: O
authors.initials: O
authors.lastname: Martinez-Maza
authors.forename: D
authors.initials: D
authors.lastname: Vredevoe
mesh_terms: D001886:Bordetella pertussis / Q000276:immunology; D002457:Cell Extracts / Q000276:immunology / Q000494:pharmacology; D016207:Cytokines / Q000096:biosynthesis*; D004168:Diphtheria Toxoid / Q000276:immunology / Q000494:pharmacology; D015721:Diphtheria-Tetanus-Pertussis Vaccine / Q000276:immunology* / Q000494:pharmacology*; D006801:Humans; D007375:Interleukin-1 / Q000032:analysis; D015850:Interleukin-6 / Q000032:analysis / Q000378:metabolism; D011112:Polymyxin B / Q000494:pharmacology; D013745:Tetanus Toxoid / Q000276:immunology / Q000494:pharmacology; D014407:Tumor Cells, Cultured / Q000187:drug effects* / Q000276:immunology*; D014409:Tumor Necrosis Factor-alpha / Q000032:analysis; D010566:Virulence Factors, Bordetella / Q000494:pharmacology
chemical_list: D002457:Cell Extracts; D016207:Cytokines; D004168:Diphtheria Toxoid; D015721:Diphtheria-Tetanus-Pertussis Vaccine; D007375:Interleukin-1; D015850:Interleukin-6; D013745:Tetanus Toxoid; D014409:Tumor Necrosis Factor-alpha; D010566:Virulence Factors, Bordetella; D011112:Polymyxin B