[Expression of BCL-2 protein in tissues and organs of the human embryo].
The bcl-2 gene product is a 24-kD protein localized in the nuclear envelope, endoplasmic reticulum, and mitochondrial membranes. Protein BCL-2 prolongs survival cells by blocking programmed cell death--apoptosis. The role of this protein in the regulation of mammalian embryo development is also suggested. BCL-2 is widely expressed early in mouse fetal development in tissue derived from all three germ layers and this expression becomes restricted with maturation. The expression of BCL-2 was studied in 20 human embryos from the 4 to 12 weeks gestation. Immunoperoxidase staining was performed using the mouse primary monoclonal antibody clone 124 (Dako, Danmark). We found the expression of BCL-2 in many organs of gastrointestinal tract, in mesenchymal cells surrounding primitive bronchial epithelium, in the cells of the metanephronic blasteme and ureteric buds. Our results indicate that bcl-2 gene could play an important role in the human embryonal tissue development.
Lichnovský
V
V
Institut für Histologie und Embryologie, Medizinische Fakultät, Palacký Universität, Olomouc, Tschechische Republik.
Kolár
Z
Z
Tauber
Z
Z
Bocek
M
M
D000818:Animals; D000911:Antibodies, Monoclonal / Q000032:analysis; D017209:Apoptosis; D016923:Cell Death; D005314:Embryonic and Fetal Development; D005260:Female; D005333:Fetus / Q000737:chemistry*; D015870:Gene Expression; D019254:Genes, bcl-2; D005865:Gestational Age; D006801:Humans; D051379:Mice; D011247:Pregnancy; D011261:Pregnancy Trimester, First; D019253:Proto-Oncogene Proteins c-bcl-2
D000911:Antibodies, Monoclonal; D019253:Proto-Oncogene Proteins c-bcl-2
Expression des BCL-2 proteins in den geweben und organen menschlicher keimlinge.