[Nucleic acids interactions with short oligonucleotide derivatives. II. Tandem of short oligonucleotides as highly sensitive system for identification of single base substitutions in target DNA].
A new approach for modification of target DNAs with tandems of derivatives of short oligonucleotides was suggested that allows highly selective modification of perfect duplexes only. At physiological temperatures, the efficiency of DNA modification by a dodecanucleotide alkylating agent was demonstrated to be the same for both perfect and mismatch-containing duplexes, whereas the tetranucleotide reagent in the presence of two flanking effectors alkylated with high selectivity the target DNA in the perfect duplex only.
Pyshnyĭ
D V
DV
Podyminogin
M A
MA
Pyshnaia
I A
IA
Lokhov
S G
SG
Ivanova
E M
EM
Zarytova
V F
VF
D000477:Alkylating Agents / Q000737:chemistry*; D001345:Autoradiography; D004247:DNA / Q000737:chemistry*; D004591:Electrophoresis, Polyacrylamide Gel; D009691:Nucleic Acid Denaturation; D009841:Oligonucleotides / Q000737:chemistry*; D010761:Phosphorus Radioisotopes; D017354:Point Mutation; D013696:Temperature
D000477:Alkylating Agents; D009841:Oligonucleotides; D010761:Phosphorus Radioisotopes; D004247:DNA
Vzaimodeĭstvie proizvodnykh korotkikh oligonukleotidov s nykleinovymi kislotami. II. Tandem korotkikh oligonukleotidov--vysokochuvstvitel'naia sistema dlia obnaruzheniia odnobukvennoĭ zameny v DNK-misheni.