A highly conserved region upstream of the fibronectin alternative exon EIIIA 3' splice site interacts with cell-type-specific nuclear proteins.
Sequencing of chicken fibronectin genomic DNA and interspecies sequence comparisons reveal a highly conserved region upstream of the alternatively spliced exon EIIIA. UV-crosslinking of RNAs corresponding to this region from the chicken and rat genes with HeLa nuclear extract demonstrates that both RNAs interact with similar proteins. However, both RNAs crosslink to a 70 kDa protein present in nuclear extracts from cells and tissues that include exon EIIIA, but not in extracts from tissues that exclude the exon. This protein represents a candidate cell-type-specific factor involved in exon EIIIA inclusion.
Norton
P A
PA
Department of Medicine, Thomas Jefferson University, Philadelphia, PA 19107, USA.
Uporova
T
T
Bennett
V D
VD
D017398:Alternative Splicing; D000818:Animals; D001483:Base Sequence; D001665:Binding Sites; D002645:Chickens; D017124:Conserved Sequence; D018076:DNA, Complementary; D005091:Exons; D005353:Fibronectins / Q000235:genetics*; D006367:HeLa Cells; D006801:Humans; D008969:Molecular Sequence Data; D009687:Nuclear Proteins / Q000378:metabolism*; D051381:Rats; D012689:Sequence Homology, Nucleic Acid
D018076:DNA, Complementary; D005353:Fibronectins; D009687:Nuclear Proteins