title: An immunodiffusion assay for the detection of canine leishmaniasis due to infection with Leishmania infantum.
abstract: An immunodiffusion assay (IDA) with polyethylene glycol (PEG) was tested for usefulness as diagnostic test for canine leishmaniasis (CL). A comparative analysis of dog sera was made using IDA with PEG, immunofluorescence assay (IFA) and enzyme immunosorbent assay (ELISA) techniques. Fourty-four dogs from Italy with CL (endemic dogs) and eight Dutch dogs with CL contracted in South Europe (expatriate dogs) were tested together with 40 endemic and 35 expatriate controls. Specificity did not differ substantially among the serotests, ELISA in endemic dogs being the least specific (mean specificity given in IFA, IDA and ELISA, 100%, 98% and 93.5%, respectively). Sensitivity in expatriate dogs was 100% for all serotests but was highly variable in endemic dogs. In parasite-negative dogs, IFA had the most sensitivity, i.e., 80.5% compared to 69% for both ELISA and IDA. In contrast, ELISA in parasite-positive endemic dogs had a sensitivity of 100% whereas both IFA and IDA gave a sensitivity of 93%. Despite its slightly lesser sensitivity than IFA or ELISA (2-6% and 5% respectively) in endemically infected dogs, IDA with PEG method may help to bring the diagnosis of CL within reach of the veterinary practitioner.
authors.affiliation: Dept. of Immunology, University of Utrecht, Netherlands.
authors.forename: W E
authors.initials: WE
authors.lastname: Bernadina
authors.forename: R
authors.initials: R
authors.lastname: De Luna
authors.forename: G
authors.initials: G
authors.lastname: Oliva
authors.forename: P
authors.initials: P
authors.lastname: Ciaramella
mesh_terms: D000818:Animals; D004283:Dog Diseases / Q000175:diagnosis* / Q000469:parasitology / Q000635:transmission; D004285:Dogs; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D005240:Feasibility Studies; D005455:Fluorescent Antibody Technique; D007118:Immunoassay / Q000379:methods*; D005779:Immunodiffusion / Q000379:methods; D007558:Italy; D018314:Leishmania infantum; D007898:Leishmaniasis, Visceral / Q000175:diagnosis / Q000635:transmission / Q000662:veterinary*; D009426:Netherlands; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity