[The action of epidermal growth factor on the development of cultured striatum cells].
BACKGROUND
Epidermic growth factor (EGF) has a neurotrophic mitogenic effect on different cell populations in the nervous system. This is modulated by the stage of development and microenvironment of the cells.


OBJECTIVE
In this paper we describe the action of EGF on embryonic striatum cells of a culture system dissociated from neurons and glias. The cell culture is prepared from 16-17 day rat embryos. In the system used, the cell population was cultured for 20-24 hours in a medium containing serum. This medium was later replaced by a mixture of specific nutrients and treated for 6 days with 20 mg/ml of EGF.


CONCLUSIONS
The substitution of serum during the initial period of development led to an appreciable reduction in the living cells in the treated cultures and in the controls. The surviving cells were mainly cellular precursors, taking into account their morphological characteristics and capacity for proliferation. The effect of EGF was seen in an increase in the number of cells and was shown to be a stimulus to the proliferation of neuronal and astrocyte precursors. The specific activity of choline acetyl-transferases determined in the cultures at 16 days showed differentiation of a cholinergic neurons subpopulation, which responded to treatment with nerve growth factor with an increase in the activity of this enzyme.
Castillo-Díaz
L
L
Laboratorio de Cultivo de Células, Ciudad de La Habana, Cuba. lazara@neurobas.sld.cu
Castellano-Benítez
O
O
Soto-Alonso
J
J
Rosillo-Martí
J C
JC
de la Cuétara-Bernal
K
K
D000818:Animals; D000911:Antibodies, Monoclonal; D016923:Cell Death; D002455:Cell Division / Q000187:drug effects; D002465:Cell Movement / Q000187:drug effects / Q000502:physiology; D002478:Cells, Cultured / Q000187:drug effects; D002795:Choline O-Acetyltransferase / Q000378:metabolism; D002799:Cholinergic Fibers / Q000187:drug effects / Q000201:enzymology; D003342:Corpus Striatum / Q000166:cytology* / Q000187:drug effects* / Q000201:enzymology; D004815:Epidermal Growth Factor / Q000494:pharmacology*; D006133:Growth Substances / Q000494:pharmacology*; D009414:Nerve Growth Factors / Q000494:pharmacology; D051381:Rats; D017208:Rats, Wistar
D000911:Antibodies, Monoclonal; D006133:Growth Substances; D009414:Nerve Growth Factors; D004815:Epidermal Growth Factor; D002795:Choline O-Acetyltransferase
Acción del factor de crecimiento epidérmico sobre el desarrollo de las células estriatales en cultivo.