Correlation of the mutation of p53 gene and the polymorphism at codon 72 in smoking-related non-small cell lung cancer patients.
The polymorphism of p53 gene at codon 72 consisting of either arginine (Arg)- or proline (Pro)-encoded allele is suggested to be associated with the susceptibility of tobacco-related lung cancer. In this study we examined the polymorphism of 224 non-small cell lung cancer (NSCLC) patients and that of 303 control persons with a polymerase chain reaction method and found that Pro-allele carriers were significantly more frequent in those patients who smoked and were affected at a younger age (<65) (P<0.05). We also investigated whether the mutational alterations of this gene could be influenced by the genotype. The overall mutation rate of 114 NSCLC patients examined with a single-strand conformation polymorphism method was 31%, which agreed with previous reports. However, the mutation rate was significantly increased in those patients who smoked and were affected at a younger age (<65) (P<0.05). Although the Pro-allele carriers among the smoker patients showed higher mutation rate than the Arg/Arg homozygotes, the difference between the genotypes had marginal significance (0.1<P<0.05) and was statistically insignificant, if the study was confined to younger patients. Thus, the present data cannot confirm a possible association of the p53 polymorphism with its mutation rate regarding smoking-related lung cancer.
Murata
M
M
Division of Epidemiology, Chiba Cancer Center Research Institute, 666-2, Nitona, Chuo-ku, Chiba, 260, Japan.
Tagawa
M
M
Kimura
H
H
Kakisawa
K
K
Shirasawa
H
H
Fujisawa
T
T
D000230:Adenocarcinoma / Q000235:genetics / Q000473:pathology; D000368:Aged; D000483:Alleles; D001120:Arginine; D002289:Carcinoma, Non-Small-Cell Lung / Q000209:etiology / Q000235:genetics* / Q000473:pathology; D002294:Carcinoma, Squamous Cell / Q000235:genetics / Q000473:pathology; D003062:Codon / Q000235:genetics; D004198:Disease Susceptibility; D005260:Female; D016158:Genes, p53; D006580:Genetic Carrier Screening; D006801:Humans; D008171:Lung Diseases / Q000235:genetics* / Q000473:pathology; D008175:Lung Neoplasms / Q000209:etiology / Q000235:genetics* / Q000473:pathology; D008297:Male; D008875:Middle Aged; D017354:Point Mutation; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic; D011392:Proline; D012307:Risk Factors; D012907:Smoking
D003062:Codon; D001120:Arginine; D011392:Proline