title: HLA class I and class II allele distribution in the Bubi population from the island of Bioko (Equatorial Guinea).
abstract: We determined the HLA frequency distribution in a sample of 100 Bubi individuals born on the island of Bioko (Equatorial Guinea). HLA-A, -B and -C typing was performed by serology and PCR-SSP. DRB1/3/4/5, DQB1 and DQA1 alleles were determined by PCR-SSOP. The HLA allele distribution of this population group resembles those found in other Bantu-speaking groups; however, the higher frequency of A30, A32, B44, DRB1*1301 in the Bubi with respect to other Bantu groups and the absence of DR4 deserve special mention. The cloning and sequencing of class I and II genes in this population allowed the description of five new allelic variants: B*4407, Cw*0706, Cw*1801, Cw*1802 and DQB1*0612 and five confirmatory sequences: B*3910, B*5703, B*8101, Cw*1203 and Cw*1701. The following new HLA-C,B haplotypes have been found in Bubi: Cw*08-B*57, Cw*18-B*57, Cw*0302-B*53, Cw*07-B*53 and Cw*1601-B*63. The most frequent seven-locus haplotype is: A*30-Cw*17-B*42-DRB1*1102-DRB3*0202-DQA1*05-DQB1 *0301. In terms of genetic distance, the Bubi are closer to other Bantu groups than to West African populations.
authors.affiliation: Servicio de Inmunología, Clínica Puerta de Hierro, Madrid, Spain. rpablo@inmuno.cph.es
authors.forename: R
authors.initials: R
authors.lastname: de Pablo
authors.forename: J M
authors.initials: JM
authors.lastname: García-Pacheco
authors.forename: C
authors.initials: C
authors.lastname: Vilches
authors.forename: M E
authors.initials: ME
authors.lastname: Moreno
authors.forename: L
authors.initials: L
authors.lastname: Sanz
authors.forename: M C
authors.initials: MC
authors.lastname: Rementería
authors.forename: S
authors.initials: S
authors.lastname: Puente
authors.forename: M
authors.initials: M
authors.lastname: Kreisler
mesh_terms: D000483:Alleles; D016703:Equatorial Guinea; D005787:Gene Frequency; D005805:Genes, MHC Class I; D005802:Genes, MHC Class II; D015235:HLA-B Antigens / Q000235:genetics; D015236:HLA-C Antigens / Q000235:genetics; D006801:Humans; D016133:Polymerase Chain Reaction; D011153:Population
chemical_list: D015235:HLA-B Antigens; D015236:HLA-C Antigens