title: Induction of follicular growth by exogenous interleukin-8.
abstract: Although the effects of inflammatory cytokines such as interleukin (IL)-1 and tumour necrosis factor (TNF)-alpha on ovulation have been investigated, there are few reports concerning the effect of IL-8 in the ovary. We examined the localization of IL-8 in the human ovary, and also investigated the number of IL-8 staining cells in a section of 1 mm2. The number of IL-8 staining cells was 10 +/- 4.8 in the proliferative phase, 2.0 +/- 1.9 in the secretary phase, and 2.4 +/- 2.2 in the postmenopausal phase respectively. The increased number of IL-8 staining cells could be shown in the human ovarian medulla/stroma during the proliferative phase. By serial section analysis of the ovary, the localization of IL-8 corresponded with mast cells. When immature rats were injected with pregnant mare serum gonadotrophin (PMSG) followed by an i.p. injection of human IL-8, ovarian vasodilatation and follicular growth were observed. Exogenous IL-8 induced a similar increase in follicular growth to that produced by the luteinizing hormone (LH) surge. At 6 h after IL-8 (20 microg) i.p., the follicular size was increased to almost the same size as human chorionic gonadotrophin (HCG) i.p. However, at 24 h after IL-8 i.p., the follicular size decreased. The size of follicular growth was almost the same size as control when <2 microg IL-8 was injected. At 6 h after IL-8 (10 microg) local injection, the follicular size in rats was similar to that observed with IL-8 (20 microg) i.p. We conclude that IL-8 is one of the important cytokines in the ovulatory process.
authors.affiliation: Department of Obstetrics and Gynaecology, Hamamatsu University School of Medicine, Shizuoka, Japan.
authors.forename: J
authors.initials: J
authors.lastname: Goto
authors.forename: N
authors.initials: N
authors.lastname: Kanayama
authors.forename: T
authors.initials: T
authors.lastname: Asahina
authors.forename: Y
authors.initials: Y
authors.lastname: Okada
authors.forename: T
authors.initials: T
authors.lastname: Kobayashi
authors.forename: T
authors.initials: T
authors.lastname: Terao
mesh_terms: D000818:Animals; D006063:Chorionic Gonadotropin / Q000494:pharmacology; D005260:Female; D006064:Gonadotropins, Equine / Q000494:pharmacology; D006801:Humans; D007124:Immunoenzyme Techniques; D016209:Interleukin-8 / Q000032:analysis / Q000494:pharmacology*; D008597:Menstrual Cycle / Q000502:physiology; D006080:Ovarian Follicle / Q000502:physiology*; D010053:Ovary / Q000098:blood supply / Q000737:chemistry; D010060:Ovulation; D017698:Postmenopause / Q000502:physiology; D051381:Rats; D017208:Rats, Wistar; D014664:Vasodilation
chemical_list: D006063:Chorionic Gonadotropin; D006064:Gonadotropins, Equine; D016209:Interleukin-8