Cloning, expression and mapping of a novel human zinc-finger gene TCF17 homologous to rodent Kid1.
We isolated a novel human zinc-finger gene TCF17 homologous to rat Kid1, a zinc-finger gene of the Krüppel type expressed predominantly in kidney. In the rat this gene seems to be a transcription factor expressed in response to renal injury and ischemia. The 2435-bp human cDNA contained an open reading frame encoding 605 amino acids. The deduced amino acid sequence showed 86.0% and 87.2% identity (91.6% and 92.8% similarity) with the rat Kid1 and mouse Tcf17 respectively. In contrast with rat Kid1, human TCF17 was expressed in all human tissues examined, including kidney. This gene was mapped by FISH to chromosome 5q35.3, where cytogenetic and molecular abnormalities have been reported often in renal cell carcinomas.
Omori
Y
Y
Otsuka GEN Research Institute, Otsuka Pharmaceutical Co., Ltd., Tokushima, Japan.
Kyushiki
H
H
Takeda
S
S
Suzuki
M
M
Kawai
A
A
Fujiwara
T
T
Takahashi
E
E
Nakamura
Y
Y
D000818:Animals; D002874:Chromosome Mapping; D003001:Cloning, Molecular; D018076:DNA, Complementary; D004268:DNA-Binding Proteins / Q000235:genetics*; D005333:Fetus; D018507:Gene Expression Regulation, Developmental; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D051379:Mice; D008969:Molecular Sequence Data; D012333:RNA, Messenger / Q000032:analysis; D051381:Rats; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D014157:Transcription Factors; D014158:Transcription, Genetic; D016335:Zinc Fingers / Q000235:genetics*
D018076:DNA, Complementary; D004268:DNA-Binding Proteins; D012333:RNA, Messenger; D014157:Transcription Factors; C410184:ZNF354A protein, human; C485822:Zfp354a protein, mouse; C485823:Zfp354a protein, rat