Structure of the human CD94 C-type lectin gene.
The genomic structure of the human CD94 gene was obtained by analyzing genomic clones obtained from two different libraries. The CD94 gene contains six exons separated by five introns. The carbohydrate-recognition domain (CRD) is encoded by three exons, and the conservation of intron positions within the CRD indicated that CD94 is closely related to group V of C-type lectins. Primer extension and S1 nuclease protection assays showed that initiation of transcription in the CD94 gene is heterogeneous, but restricted to a 60 base pair region around the major initiation site enclosed within a putative initiator element (TTA+1TTCA). The study of the promoter region of CD94 may help to understand the selective expression of this C-type lectin glycoprotein on NK cells and subsets of cytotoxic T cells.
Rodríguez
A
A
Hospital de la Princesa, Universidad Autónoma de Madrid, Servicio de Inmunología, Madrid, Spain.
Carretero
M
M
Glienke
J
J
Bellón
T
T
Ramírez
A
A
Lehrach
H
H
Francis
F
F
López-Botet
M
M
D015703:Antigens, CD / Q000737:chemistry* / Q000235:genetics* / Q000302:isolation & purification; D001402:B-Lymphocytes; D001483:Base Sequence; D002460:Cell Line; D003001:Cloning, Molecular / Q000379:methods; D005091:Exons; D006801:Humans; D007438:Introns; D019169:Jurkat Cells; D037102:Lectins / Q000737:chemistry / Q000235:genetics* / Q000302:isolation & purification; D037181:Lectins, C-Type; D008562:Membrane Glycoproteins / Q000737:chemistry* / Q000235:genetics* / Q000302:isolation & purification; D008969:Molecular Sequence Data; D051920:NK Cell Lectin-Like Receptor Subfamily D; D010442:Peptide Chain Initiation, Translational / Q000235:genetics / Q000276:immunology; D014158:Transcription, Genetic / Q000276:immunology
D015703:Antigens, CD; C497869:KLRD1 protein, human; D037102:Lectins; D037181:Lectins, C-Type; D008562:Membrane Glycoproteins; D051920:NK Cell Lectin-Like Receptor Subfamily D