[The morphofunctional specialization of the main and accessory magnocellular neuroendocrine nuclei of the hypothalamus].
Intensity of vasopressin gene expression was compared in neurosecretory cells of supraoptic, postoptic, paraventricular and 6 accessory nuclei (extrahypothalamic included) of adult intact Wistar rats to study the morphofunctional specialization of magnocellular hypothalamic nuclei. Messenger RNA (mRNA) was revealed by radioautographic in situ hybridization, using 35S labelled exonic-intronic probe. By densitometry the amount of label was shown not to differ significantly in neurosecretory cells of all nuclei studied. The latter reflects similar basal level of vasopressin gene expression in all magnocellular nuclei of intact rats. The explanations of this fact as well as possible perspectives of studying molecular biological basis of functional specialization of different magnocellular hypothalamic formations are discussed.
Grinevich
V V
VV
Polenov
A L
AL
D000818:Animals; D001345:Autoradiography; D003720:Densitometry / Q000379:methods; D005786:Gene Expression Regulation / Q000502:physiology; D007031:Hypothalamus / Q000166:cytology* / Q000502:physiology; D017403:In Situ Hybridization / Q000379:methods; D008297:Male; D009490:Neurosecretory Systems / Q000166:cytology* / Q000502:physiology; D012333:RNA, Messenger / Q000235:genetics; D051381:Rats; D017208:Rats, Wistar; D014667:Vasopressins / Q000235:genetics
D012333:RNA, Messenger; D014667:Vasopressins
Morfofunktsional'naia spetsializatsiia glavnykh i dobavochnykh krupnokletochnykh neĭroéndokrinnykh iader gipotalamusa.