Antigenicity of deoxyadenosine 5'-monophosphate cross-linked with polyamine.
Deoxyadenosine 5'-monophosphate was cross-linked to spermine using glutaraldehyde as cross-linking reagent. The complex thus obtained was characterized by UV absorption spectroscopy, size-exclusion chromatography and CD. The complex had a molecular mass of around 10,200 Da. Antibodies were raised against this complex in rabbits and the titre obtained was found to be > 1:3200. The fine specificity of the raised antibodies was checked by competition ELISA. The complex also bound to naturally occurring anti-DNA autoantibodies, suggesting a possible role of the complex in the pathogenesis of systemic lupus erythematosus.
Ahmad
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Department of Biochemistry, J. N. Medical College, Aligarh Muslim University, India.
Ahmad
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Ali
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D000818:Animals; D000974:Antibodies, Antinuclear / Q000737:chemistry / Q000276:immunology*; D000918:Antibody Specificity / Q000276:immunology*; D000936:Antigen-Antibody Complex; D002942:Circular Dichroism; D003432:Cross-Linking Reagents; D004247:DNA / Q000235:genetics / Q000276:immunology; D003838:Deoxyadenine Nucleotides / Q000737:chemistry / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay; D005976:Glutaral; D008180:Lupus Erythematosus, Systemic / Q000235:genetics / Q000276:immunology*; D008970:Molecular Weight; D011817:Rabbits; D013056:Spectrophotometry, Ultraviolet; D013096:Spermine / Q000737:chemistry
D000974:Antibodies, Antinuclear; D000936:Antigen-Antibody Complex; D003432:Cross-Linking Reagents; D003838:Deoxyadenine Nucleotides; D013096:Spermine; C012873:2'-deoxy-5'-adenosine monophosphate; D004247:DNA; D005976:Glutaral