Affinity capillary electrophoresis.
The application of affinity capillary electrophoresis (ACE) to the study of molecular interactions is reviewed. ACE appears to be a sensitive, versatile and convenient tool to obtain reliable data on binding constants and stoichiometries of interacting systems using the Hummel-Dreyer method and variants thereof. A powerful feature is the possibility to analyze simultaneously the affinity of a large number of compounds for the same ligand, making it a promising tool for the screening of large combinatorial libraries.
Rippel
G
G
Delft University of Technology, Department of Biochemical Engineering, The Netherlands.
Corstjens
H
H
Billiet
H A
HA
Frank
J
J
D000595:Amino Acid Sequence; D004247:DNA / Q000032:analysis; D019075:Electrophoresis, Capillary / Q000379:methods*; D006801:Humans; D007700:Kinetics; D008969:Molecular Sequence Data; D010455:Peptides / Q000032:analysis; D011506:Proteins / Q000032:analysis
D010455:Peptides; D011506:Proteins; D004247:DNA