Enhancer effect of fluorescein on the luminol-H2O2-horseradish peroxidase chemiluminescence: energy transfer process.
The chemiluminescence of the luminol-H2O2-horseradish peroxidase system is increased by fluorescein. Fluorescein produces an enhancement of the luminol chemiluminescence similar to that of phenolphthalein, by an energy transfer process from luminol to fluorescein. The maximum intensity and the total chemiluminescence emission (between 380 and 580 nm) of luminol with fluorescein was more than three times greater than without fluorescein; however, the emission duration was shorter. The emission spectra in the presence of fluorescein had two maxima (425 and 535 nm) and the enhancement was dependent on pH and fluorescein concentration. A mechanism is proposed to explain these effects.
Navas Díaz
A
A
Departamento de Química Analítica, Facultad de Ciencias, Universidad de Málaga, Spain.
González García
J A
JA
Lovillo
J
J
D004735:Energy Transfer; D019793:Fluorescein; D006735:Horseradish Peroxidase; D006861:Hydrogen Peroxide; D006863:Hydrogen-Ion Concentration; D007202:Indicators and Reagents; D008163:Luminescent Measurements; D008165:Luminol; D012680:Sensitivity and Specificity; D013050:Spectrometry, Fluorescence / Q000379:methods
D007202:Indicators and Reagents; D008165:Luminol; D006861:Hydrogen Peroxide; D006735:Horseradish Peroxidase; D019793:Fluorescein