[DNA sequencing of human ciliary neurotropic factor gene by silver staining method].
OBJECTIVE
To introduce a non-radioactive protocol for DNA sequence analysis which employs a silver staining procedure


METHODS
DNA template is amplified by Taq DNA polymerase to generate sequence ladder, No labeled nucleotide or primer is involved in the thermal-cycling sequencing reaction. A sensitive silver staining procedure is employed to visualize bands in sequencing gel.


RESULTS
This silver staining method is used for DNA sequence analysis of human cilliary neurotrophic factor (hCNTF) gene. The resulted band resolution is comparable to radioactive sequencing method, and data can be obtain within less than 2 hours after sequencing reaction.


CONCLUSIONS
Silver staining is a rapid, inexpensive and reliable method for DNA sequence analysis.
Fu
X
X
Institute of Medical Sciences, Nanjing Railway Medical College, Nanjing, 210009 P. R. China.
Huang
X
X
Gao
Y
Y
Hu
X
X
D020934:Ciliary Neurotrophic Factor; D006801:Humans; D009419:Nerve Tissue Proteins / Q000235:genetics*; D017422:Sequence Analysis, DNA / Q000379:methods*; D016622:Silver Staining
D020934:Ciliary Neurotrophic Factor; D009419:Nerve Tissue Proteins