title: Alternative splicing of GTBP in normal human tissues.
abstract: GTBP is one of the functional homologues of the bacterial mismatch repair gene mutS, whose product forms a heterodimer with hMSH2 to bind to the mismatched region of double-stranded DNA. We determined the expression of the GTBP gene and found that two forms of the transcripts were ubiquitously expressed in normal human tissues. These transcripts, termed GTBP-N and GTBP-ALT, were generated by the alternative splicing machinery. These two transcripts share 3172 bp from the translation initiation codon that codes for 1057 amino acids. GTBP-ALT lacked a region that codes for a highly conserved region between GTBP-N, hMSH2, and hMSH3 in their C-termini.
authors.affiliation: Department of Molecular Pathology, Tohoku University School of Medicine, Sendai, Japan.
authors.forename: H O
authors.initials: HO
authors.lastname: Shiwaku
authors.forename: S
authors.initials: S
authors.lastname: Wakatsuki
authors.forename: Y
authors.initials: Y
authors.lastname: Mori
authors.forename: S
authors.initials: S
authors.lastname: Fukushige
authors.forename: A
authors.initials: A
authors.lastname: Horii
mesh_terms: D000199:Actins; D017398:Alternative Splicing / Q000235:genetics*; D000595:Amino Acid Sequence; D001483:Base Sequence; D015152:Blotting, Northern; D001921:Brain; D003360:Cosmids; D004260:DNA Repair / Q000235:genetics*; D004268:DNA-Binding Proteins / Q000235:genetics; D005091:Exons; D015723:Gene Library; D006801:Humans; D007438:Introns; D008969:Molecular Sequence Data; D012091:Repetitive Sequences, Nucleic Acid; D014018:Tissue Distribution
chemical_list: D000199:Actins; D004268:DNA-Binding Proteins