Coordination of initiation of nuclear division and initiation of cell division in Schizosaccharomyces pombe: genetic interactions of mutations.
sep1+ encodes a Schizosaccharomyces pombe homolog of the HNF-3/forkhead family of the tissue-specific and developmental gene regulators identified in higher eukaryotes. Its mutant allele sep1-1 causes a defect in cytokinesis and confers a mycelial morphology. Here we report on genetic interactions of sep1-1 with the M-phase initiation mutations wee1-, cdc2-1w, and cdc25-22. The double mutants sep1-1 wee1- and sep1-1 cdc2-1w form dikaryon cells at high frequency, which is due to nuclear division in the absence of cell division. The dikaryosis is reversible and suppressible by cdc25-22. We propose that the genes wee1+, cdc2+, cdc25+, and sep1+ form a regulatory link between the initiation of mitosis and the initiation of cell division.
Grallert
A
A
Department of Genetics, University of Debrecen, Hungary.
Grallert
B
B
Ribar
B
B
Sipiczki
M
M
D000199:Actins / Q000302:isolation & purification; D016203:CDC2 Protein Kinase / Q000235:genetics; D018797:Cell Cycle Proteins / Q000235:genetics; D002455:Cell Division / Q000235:genetics; D002467:Cell Nucleus / Q000235:genetics / Q000648:ultrastructure; D005095:Exoribonucleases / Q000235:genetics; D005434:Flow Cytometry; D005455:Fluorescent Antibody Technique; D008957:Models, Genetic; D009154:Mutation; D009687:Nuclear Proteins; D010749:Phosphoprotein Phosphatases / Q000235:genetics; D011505:Protein-Tyrosine Kinases / Q000235:genetics; D029701:Saccharomyces cerevisiae Proteins; D012568:Schizosaccharomyces / Q000166:cytology* / Q000235:genetics*; D029702:Schizosaccharomyces pombe Proteins; D013172:Spores, Fungal; D020687:cdc25 Phosphatases
D000199:Actins; D018797:Cell Cycle Proteins; D009687:Nuclear Proteins; D029701:Saccharomyces cerevisiae Proteins; D029702:Schizosaccharomyces pombe Proteins; C068689:wee1 protein, S pombe; D011505:Protein-Tyrosine Kinases; D016203:CDC2 Protein Kinase; D005095:Exoribonucleases; C068398:XRN1 protein, S cerevisiae; D010749:Phosphoprotein Phosphatases; D020687:cdc25 Phosphatases