title: Results of long-term carcinogenicity studies of chlorine in rats.
abstract: Four groups, each of 50 male and 50 female Sprague-Dawley rats, of the colony used in the Cancer Research Center of Bentivoglio of the Ramazzini Foundation, 12 weeks old at the start of the study, received drinking water containing sodium hypochlorite, resulting in concentrations of active chlorine of 750, 500, and 100 mg/l (treated groups), and tap water (active chlorine < 0.2 mg/l) (control group), respectively, for 104 weeks. Among the female rats of the treated groups, an increased incidence of lymphomas and leukemias has been observed, although this is not clearly dose related. Moreover, sporadic cases of some tumors, the occurrence of which is extremely unusual among the untreated rats of the colony used (historical controls), were detected in chlorine-exposed animals. The results of this study confirm the results of the experiment of the United States National Toxicology Program (1991), which showed an increase of leukemia among female Fischer 344/N rats following the administration of chlorine (in the form of sodium hypochlorite and chloramine) in their drinking water. The data here presented call for further research aimed at quantifying the oncogenic risks related to the chlorination of drinking water, to be used as a basis for consequent public health measures.
authors.affiliation: Cancer Research Center, European Ramazzini Foundation of Oncology and Environmental Sciences, Bentivoglio Castle, Italy.
authors.forename: M
authors.initials: M
authors.lastname: Soffritti
authors.forename: F
authors.initials: F
authors.lastname: Belpoggi
authors.forename: A
authors.initials: A
authors.lastname: Lenzi
authors.forename: C
authors.initials: C
authors.lastname: Maltoni
mesh_terms: D000818:Animals; D001835:Body Weight; D002273:Carcinogens; D002713:Chlorine / Q000633:toxicity*; D004326:Drinking; D005247:Feeding Behavior; D005260:Female; D008297:Male; D009374:Neoplasms, Experimental / Q000139:chemically induced; D051381:Rats; D017207:Rats, Sprague-Dawley; D012973:Sodium Hypochlorite / Q000633:toxicity*; D013997:Time Factors; D014881:Water Supply / Q000592:standards*
chemical_list: D002273:Carcinogens; D002713:Chlorine; D012973:Sodium Hypochlorite