Expression of Xa1, a bacterial blight-resistance gene in rice, is induced by bacterial inoculation.
The Xa1 gene in rice confers resistance to Japanese race 1 of Xanthomonas oryzae pv. oryzae, the causal pathogen of bacterial blight (BB). We isolated the Xa1 gene by a map-based cloning strategy. The deduced amino acid sequence of the Xa1 gene product contains nucleotide binding sites (NBS) and a new type of leucine-rich repeats (LRR); thus, Xa1 is a member of the NBS-LRR class of plant disease-resistance genes, but quite different from Xa21, another BB-resistance gene isolated from rice. Interestingly, Xa1 gene expression was induced on inoculation with a bacterial pathogen and wound, unlike other isolated resistance genes in plants, which show constitutive expression. The induced expression may be involved in enhancement of resistance against the pathogen.
Yoshimura
S
S
Rice Genome Research Program, National Institute of Agrobiological Resources/Society for Techno-innovation of Agriculture, Forestry and Fisheries, Kannondai, Tsukuba, Ibaraki 305, Japan. satomi@bs.aist-nara.ac.jp
Yamanouchi
U
U
Katayose
Y
Y
Toki
S
S
Wang
Z X
ZX
Kono
I
I
Kurata
N
N
Yano
M
M
Iwata
N
N
Sasaki
T
T
D000595:Amino Acid Sequence; D001665:Binding Sites; D003001:Cloning, Molecular; D003360:Cosmids; D018076:DNA, Complementary / Q000235:genetics; D015870:Gene Expression; D017343:Genes, Plant; D005816:Genetic Complementation Test; D007113:Immunity, Innate / Q000235:genetics; D008969:Molecular Sequence Data; D009711:Nucleotides / Q000378:metabolism; D012275:Oryza / Q000235:genetics* / Q000382:microbiology; D010935:Plant Diseases / Q000235:genetics*; D012333:RNA, Messenger / Q000235:genetics; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid; D015398:Signal Transduction; D013045:Species Specificity
D018076:DNA, Complementary; D009711:Nucleotides; D012333:RNA, Messenger