The antibiotic ceftazidime is a singlet oxygen quencher as demonstrated by ultra-weak chemiluminescence and by inhibition of AAP consumption.
We demonstrated that the cephalosporin antibiotic ceftazidime (CAZ) deactivated singlet oxygen (1O2). We then studied the mechanisms of the CAZ effects on the ultra weak chemiluminescence (uwCL) associated with the energy decay of 1O2 generated by the Mallet reaction (H2O2 + HOCl --> HCl + H2O + 1O2), and on the anthracene-9,10-dipropionic acid (AAP) consumption by 1O2 generated by irradiation of Rose Bengal (RB). The uwCL generated by the Mallet reaction was amplified (6.2 times) by CAZ. The use of red and blue filters, which absorb radiation below 610 nm and between 470 and 700 nm respectively, demonstrated that CAZ increased the uwCL by a radiation emission at wavelengths shorter than the 633 and 704 nm wavelength emissions of 1O2. CAZ was excited by scavenging the energy excess of 1O2, which so returned to its fundamental state, while CAZ deactivated with light emission between 430-480 nm. CAZ also inhibited in a dose-dependent manner the consumption of AAP by 1O2 generated by the irradiation of RB. The protection of AAP by 5 x 10(-3) M CAZ was equivalent to that of 10(-3) M histidine and 3 X 10(-6) M sodium azide. This process of 1O2 deactivation will be useful in diseases characterized by an excessive PMN activation with a release of activated oxygen species.
Deby-Dupont
G
G
Centre for the Biochemistry of Oxygen, Institute de Chimie, B6a, Domaine Universitaire du Sart Tilman, Liège, Belgium. gdeby@ulg.ac.be
Deby
C
C
Mouithys-Mickalad
A
A
Hoebeke
M
M
Mathy-Hartert
M
M
Jadoul
L
L
Vandenberghe
A
A
Lamy
M
M
D000873:Anthracenes / Q000378:metabolism*; D000900:Anti-Bacterial Agents; D002442:Ceftazidime / Q000378:metabolism*; D002511:Cephalosporins / Q000378:metabolism; D016166:Free Radical Scavengers / Q000378:metabolism; D006639:Histidine / Q000378:metabolism; D006861:Hydrogen Peroxide / Q000378:metabolism; D006997:Hypochlorous Acid / Q000378:metabolism; D008027:Light; D008163:Luminescent Measurements; D010100:Oxygen / Q000378:metabolism*; D010787:Photosensitivity Disorders; D011422:Propionates / Q000378:metabolism*; D017382:Reactive Oxygen Species / Q000378:metabolism; D012395:Rose Bengal / Q000378:metabolism; D026082:Singlet Oxygen; D019810:Sodium Azide / Q000378:metabolism; D013053:Spectrophotometry
D000873:Anthracenes; D000900:Anti-Bacterial Agents; D002511:Cephalosporins; D016166:Free Radical Scavengers; D011422:Propionates; D017382:Reactive Oxygen Species; C110869:anthracene-9,10-dipropionic acid; D026082:Singlet Oxygen; D012395:Rose Bengal; D006639:Histidine; D006997:Hypochlorous Acid; D019810:Sodium Azide; D002442:Ceftazidime; D006861:Hydrogen Peroxide; D010100:Oxygen