Immunological aspects of the common food colorants, amaranth and tartrazine.
We describe a sensitive and reproducible microassay model using human peripheral blood lymphocytes (PBL) for discrimination between the cytotoxic and immunosuppressive effects of food colorants such as amaranth and tartrazine. The cytotoxic effects of a wide range of concentrations of these substances were studied on human PBL by the colorimetric in vitro cytotoxicity assays, neutral red uptake (NR) and thiazolyl blue tetrazolium bromide (MTT). The immunotoxic properties of these 2 substances were determined by a [3H]-thymidine DNA incorporation assay on phytohemagglutinin stimulated or non-stimulated lymphocytes, as well as by a Cr51 release Natural Killer assays. The results showed clear immunosuppressive effects from the 2 substances tested, although the concentrations chosen for this study proved to be non-cytotoxic by NR and MTT cytotoxic endpoints.
Koutsogeorgopoulou
L
L
Department of Forensic Medicine and Toxicology, School of Medicine, Athens, Greece.
Maravelias
C
C
Methenitou
G
G
Koutselinis
A
A
D000328:Adult; D000548:Amaranth Dye / Q000633:toxicity*; D002455:Cell Division / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D003602:Cytotoxicity, Immunologic / Q000187:drug effects; D004247:DNA / Q000096:biosynthesis; D005505:Food Coloring Agents / Q000633:toxicity*; D006801:Humans; D007694:Killer Cells, Natural / Q000187:drug effects / Q000276:immunology; D008212:Lymphocyte Depletion; D009499:Neutral Red; D010835:Phytohemagglutinins; D013601:T-Lymphocytes / Q000187:drug effects* / Q000276:immunology; D013645:Tartrazine / Q000633:toxicity*; D013778:Tetrazolium Salts; D013844:Thiazoles; D013936:Thymidine
D005505:Food Coloring Agents; D010835:Phytohemagglutinins; D013778:Tetrazolium Salts; D013844:Thiazoles; D009499:Neutral Red; D004247:DNA; D000548:Amaranth Dye; C022616:thiazolyl blue; D013645:Tartrazine; D013936:Thymidine