A simple method for fixing, dehydrating and embedding pollen tubes cultivated in vitro for optical and transmission electron microscopy.
A simple method to cultivate pollen tubes in a gelatin medium is presented. After the growth of the pollen tubes in the culture medium, they are fixed, dehydrated, and embedded in resin for ultramicrotomy. The method is easy and does not require the purchase of special materials beyond those needed for the usual techniques for studying biological specimens under transmission electron microscopy.
dos Santos
R P
RP
Department of Botany, Institute of Biosciences, Universidade Federal do Rio Grande do Sul-UFRGS, Brazil.
de Araujo Mariath
J E
JE
D008854:Microscopy, Electron; D008867:Microtomy; D011058:Pollen / Q000648:ultrastructure*; D016610:Tissue Embedding / Q000379:methods; D016707:Tissue Fixation / Q000379:methods