title: Expression of matrix metalloproteinase-1 mRNA related to eosinophilia and interleukin-5 gene expression in head and neck tumour tissue.
abstract: In situ hybridization (ISH) of the expression of matrix metalloproteinase-1 (MMP-1) in areas of local invasion of head and neck tumours was performed. Expression of MMP-1 mRNA was found in stromal inflammatory cells adjacent to the tumour nests, apparently in eosinophils. MMP-1 mRNA expression was seen only in cases with moderate or greater stromal eosinophilia. MMP-1 mRNA was detected in benign papilloma and verrucous carcinoma. In squamous cell carcinomas, expression of the gene was detected only in well-differentiated cases. Expression of MMP-1 mRNA was not related to the grade of malignancy, and appeared in stromal eosinophils, suggesting to us that it is involved in a remodelling process in the reaction of the host to tumour invasion. ISH examination of the expression of interleukin-5 (IL-5), the activating factor for eosinophils, was also performed. In the tumour, its expression was consistent with the location of eosinophils, as with MMP-1 mRNA, suggesting an autocrine mechanism.
authors.affiliation: Department of Otolaryngology, School of Medicine, Keio University, Tokyo, Japan.
authors.forename: Y
authors.initials: Y
authors.lastname: Ono
authors.forename: M
authors.initials: M
authors.lastname: Fujii
authors.forename: K
authors.initials: K
authors.lastname: Kameyama
authors.forename: Y
authors.initials: Y
authors.lastname: Otani
authors.forename: Y
authors.initials: Y
authors.lastname: Sakurai
authors.forename: J
authors.initials: J
authors.lastname: Kanzaki
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D002294:Carcinoma, Squamous Cell / Q000201:enzymology / Q000473:pathology; D018289:Carcinoma, Verrucous / Q000201:enzymology / Q000473:pathology; D017364:Collagenases / Q000235:genetics / Q000378:metabolism*; D004802:Eosinophilia / Q000201:enzymology* / Q000473:pathology; D004804:Eosinophils / Q000201:enzymology*; D005260:Female; D006258:Head and Neck Neoplasms / Q000201:enzymology* / Q000473:pathology; D006801:Humans; D017403:In Situ Hybridization; D015848:Interleukin-5 / Q000378:metabolism*; D008297:Male; D020781:Matrix Metalloproteinase 1; D008875:Middle Aged; D010212:Papilloma / Q000201:enzymology; D012333:RNA, Messenger / Q000378:metabolism*
chemical_list: D015848:Interleukin-5; D012333:RNA, Messenger; D017364:Collagenases; D020781:Matrix Metalloproteinase 1