CD146: biosynthesis and production of a soluble form in human cultured endothelial cells.
We previously identified the S-Endo 1-associated antigen (CD146), an endothelial member of the immunoglobulin superfamily with a characteristic V-V-C2-C2-C2 Ig domain structure. In cultured human endothelial cells, we investigated its biosynthesis by immunoprecipitation and pulse-chase labeling. CD146 was synthesized as a 100 kDa precursor form, which was processed into a 120 kDa mature form. In the culture media of endothelial cells, we observed a CD146 soluble form that was about 10 kDa smaller than cell-associated CD146. In parallel with soluble forms of other members of the immunoglobulin superfamily, soluble CD146 could modulate and control the functions of the molecule.
Bardin
N
N
Laboratoire d'Hématologie et d'Immunologie, U.F.R. de Pharmacie, Marseille, France.
Francès
V
V
Combes
V
V
Sampol
J
J
Dignat-George
F
F
D015703:Antigens, CD; D000954:Antigens, Surface / Q000096:biosynthesis* / Q000302:isolation & purification; D015153:Blotting, Western; D051929:CD146 Antigen; D002478:Cells, Cultured; D004591:Electrophoresis, Polyacrylamide Gel; D004730:Endothelium, Vascular / Q000276:immunology*; D006801:Humans; D008562:Membrane Glycoproteins; D008970:Molecular Weight; D019006:Neural Cell Adhesion Molecules; D010449:Peptide Mapping; D014471:Umbilical Veins
D015703:Antigens, CD; D000954:Antigens, Surface; D051929:CD146 Antigen; C497902:MCAM protein, human; D008562:Membrane Glycoproteins; D019006:Neural Cell Adhesion Molecules