title: The messenger RNAs for both glial cell line-derived neurotrophic factor receptors, c-ret and GDNFRalpha, are induced in the rat brain in response to kainate-induced excitation.
abstract: Glial cell line-derived neurotrophic factor (GDNF) has two receptors, receptor-tyrosine kinase c-ret and glycosylphosphatidylinositol-linked cell surface receptor GDNFRalpha. Kainate-induced seizures, a widely studied model of neuronal plasticity and human epilepsy, have been shown to increase gene expression of several trophic factors, including GDNF, in the rat hippocampus. Here we show that systemic kainate-induced excitation leads to a transient increase of both c-ret and GDNFRalpha messenger RNAs in the rat brain. Northern analysis demonstrated that, in the hippocampus, the maximal 2.5-fold increase of c-ret and four-fold increase of GDNFRalpha messenger RNAs was observed after 12 h of kainate injection, in contrast to GDNF messenger RNA, which reaches its maximum in 4-6 h. The blocking of de novo protein synthesis by cycloheximide inhibited the induction of GDNF receptors by kainate, whereas blocking of the N-methyl-D-aspartate-type glutamate receptors by the antagonist dizocilpine maleate did not significantly alter the response. Thus, GDNF receptor messenger RNA increase by kainate depends on protein synthesis, but is not mediated by the N-methyl-D-aspartate receptor. GDNFRalpha and c-ret show distinct, but partially overlapping, patterns of expression in the brain after kainate treatment. GDNFRalpha messenger RNA was prominently induced in the dentate gyrus of the rat hippocampus, less in the habenular and reticular thalamic nuclei and cerebral cortex as revealed by in situ hybridization. C-ret transcripts were induced in the hilus of the hippocampus, several thalamic and amygdala nuclei and in superficial layers of the piriform cortex. These data suggest that GDNF and its receptors may play a local role in neuronal plasticity and in neuronal protection following epileptic insults.
authors.affiliation: Institute of Biotechnology, Program of Molecular Neurobiology, University of Helsinki, Finland.
authors.forename: M
authors.initials: M
authors.lastname: Reeben
authors.forename: A
authors.initials: A
authors.lastname: Laurikainen
authors.forename: J O
authors.initials: JO
authors.lastname: Hiltunen
authors.forename: E
authors.initials: E
authors.lastname: Castrén
authors.forename: M
authors.initials: M
authors.lastname: Saarma
mesh_terms: D000818:Animals; D015152:Blotting, Northern; D001923:Brain Chemistry / Q000187:drug effects / Q000502:physiology*; D015342:DNA Probes; D029721:Drosophila Proteins; D018691:Excitatory Amino Acid Antagonists / Q000494:pharmacology*; D051097:Glial Cell Line-Derived Neurotrophic Factor Receptors; D006624:Hippocampus / Q000187:drug effects / Q000378:metabolism; D017403:In Situ Hybridization; D007608:Kainic Acid / Q000494:pharmacology*; D008297:Male; D011518:Proto-Oncogene Proteins / Q000096:biosynthesis* / Q000235:genetics; D051096:Proto-Oncogene Proteins c-ret; D015347:RNA Probes; D012333:RNA, Messenger / Q000096:biosynthesis*; D051381:Rats; D017208:Rats, Wistar; D020794:Receptor Protein-Tyrosine Kinases / Q000096:biosynthesis* / Q000235:genetics
chemical_list: D015342:DNA Probes; D029721:Drosophila Proteins; D018691:Excitatory Amino Acid Antagonists; D051097:Glial Cell Line-Derived Neurotrophic Factor Receptors; D011518:Proto-Oncogene Proteins; D015347:RNA Probes; D012333:RNA, Messenger; D051096:Proto-Oncogene Proteins c-ret; D020794:Receptor Protein-Tyrosine Kinases; C416649:Ret protein, Drosophila; C495001:Ret protein, rat; D007608:Kainic Acid