title: High-performance liquid chromatographic determination of polyamines in milk as their 9-fluorenylmethoxycarbonyl derivatives using a column-switching technique.
abstract: A high-performance liquid chromatographic method for the determination of polyamines in milk is milk is described. Polyamines were extracted in perchloric acid and derivatized with 9-fluorenylmethoxycarbonyl chloride (FMOC-Cl). The excess of reagent was reacted with aspartic acid before the analysis on a column-switching system. Linearity of derivatization was calculated for each amine and the coefficient of regression ranged from 0.994 to 0.999. Chromatographic separation of FMOC-polyamines was achieved with a gradient elution programme of water-acetonitrile. The correlation coefficients of the standard curves in the concentration range from 0.5 to 5 nmol ml-1 were higher than 0.991. The repeatability of the method, expressed as R.S.D. for each polyamines ranged from 3.0 to 8.6%. The percent mean recoveries at 1 nmol ml-1 spiking level were 49 +/- 3, 58 +/- 5, 61 +/- 5 and 48 +/- 4 for putrescine, cadaverine, spermidine and spermine, respectively. The limit of detection, calculated on the basis of three times signal-to-noise ratio, was 50 pmol ml-1 for each polyamine.
authors.affiliation: Istituto di Zootecnica Veterinaria, Milan, Italy.
authors.forename: F
authors.initials: F
authors.lastname: Bellagamba
authors.forename: V M
authors.initials: VM
authors.lastname: Moretti
authors.forename: T
authors.initials: T
authors.lastname: Mentasti
authors.forename: A
authors.initials: A
authors.lastname: Albertini
authors.forename: U
authors.initials: U
authors.lastname: Luzzana
authors.forename: F
authors.initials: F
authors.lastname: Valfrè
mesh_terms: D000818:Animals; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D005449:Fluorenes / Q000737:chemistry*; D007202:Indicators and Reagents / Q000737:chemistry*; D016014:Linear Models; D008892:Milk / Q000737:chemistry*; D011073:Polyamines / Q000032:analysis* / Q000737:chemistry; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
chemical_list: D005449:Fluorenes; D007202:Indicators and Reagents; D011073:Polyamines; C054007:1-(9-fluorenyl)methyl chloroformate