Analysis of TH1 and TH2 cytokine production in low grade B cell gastric MALT-type lymphomas stimulated in vitro with Helicobacter pylori.
Previous studies have suggested that the dependence of low grade B cell gastric lymphoma on infection of the gastric mucosa with Helicobacter pylori results from help provided by H pylori specific tumour infiltrating T cells. ELISPOT analysis was used to characterise functional subpopulations of tumour infiltrating T cells. The production of the TH1 cytokine interferon gamma and TH2 cytokines interleukin (IL)-4, IL-5, and IL-10 were measured in tumour cell suspensions from two cases of low grade B cell gastric lymphoma, one case of thyroid gland lymphoma, and one case of salivary gland lymphoma. Cells were assayed on day 0 and following 24 hours incubation either in culture medium or with a range of strains of H pylori. There was a dominant TH1-type (pro-inflammatory) response consistent with the TH1 response observed in H pylori gastritis.
Hauer
A C
AC
Department of Paediatric Gastroenterology, St Bartholomew's School of Medicine, St Bartholomew's Hospital, London, UK.
Finn
T M
TM
MacDonald
T T
TT
Spencer
J
J
Isaacson
P G
PG
D000942:Antigens, Bacterial / Q000276:immunology*; D016207:Cytokines / Q000096:biosynthesis*; D016480:Helicobacter pylori / Q000276:immunology*; D006801:Humans; D007371:Interferon-gamma / Q000096:biosynthesis; D007378:Interleukins / Q000096:biosynthesis; D018442:Lymphoma, B-Cell, Marginal Zone / Q000276:immunology*; D009363:Neoplasm Proteins / Q000096:biosynthesis*; D013274:Stomach Neoplasms / Q000276:immunology*; D018417:Th1 Cells / Q000276:immunology; D018418:Th2 Cells / Q000276:immunology; D014407:Tumor Cells, Cultured
D000942:Antigens, Bacterial; D016207:Cytokines; D007378:Interleukins; D009363:Neoplasm Proteins; D007371:Interferon-gamma