Interactions of drebrin and gephyrin with profilin.
Profilin is an actin monomer-binding protein which stimulates actin polymerization. Recent studies have revealed that profilin interacts with VASP, Mena, Bnilp, Bnrlp, and mDia, all of which have the proline-rich domain. Here, we isolated three profilin-binding proteins from rat brain cytosol by glutathione S-transferase-profilin affinity column chromatography and identified them as Mena, drebrin, and gephyrin. These proteins had a proline-rich domain and directly interacted with profilin.
Mammoto
A
A
Department of Molecular Biology and Biochemistry, Osaka University Medical School, Suita, Japan.
Sasaki
T
T
Asakura
T
T
Hotta
I
I
Imamura
H
H
Takahashi
K
K
Matsuura
Y
Y
Shirao
T
T
Takai
Y
Y
D000595:Amino Acid Sequence; D000818:Animals; D001665:Binding Sites / Q000235:genetics; D001921:Brain / Q000378:metabolism; D002352:Carrier Proteins / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D003285:Contractile Proteins; D003600:Cytosol / Q000378:metabolism; D006801:Humans; D066298:In Vitro Techniques; D008565:Membrane Proteins / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D008840:Microfilament Proteins / Q000378:metabolism*; D008969:Molecular Sequence Data; D009479:Neuropeptides / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D010449:Peptide Mapping; D051304:Profilins; D011392:Proline / Q000737:chemistry; D011485:Protein Binding; D051381:Rats; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism
D002352:Carrier Proteins; D003285:Contractile Proteins; D008565:Membrane Proteins; D008840:Microfilament Proteins; D009479:Neuropeptides; C495738:PFN1 protein, human; C495740:Pfn1 protein, rat; D051304:Profilins; D011993:Recombinant Fusion Proteins; C053386:drebrins; C073441:gephyrin; D011392:Proline