Flexibility of the B-DNA backbone: effects of local and neighbouring sequences on pyrimidine-purine steps.
The structurally correlated dihedral angles epsilon and zeta are known for their large variability within the B-DNA backbone. We have used molecular modelling to study both energetic and mechanical features of these variations which can produce BI/BII transitions. Calculations were carried out on DNA oligomers containing either YpR or RpY dinucleotides steps within various sequence environments. The results indicate that CpA and CpG steps favour the BI/BII transition more than TpA or any RpY step. The stacking energy and its intra- and inter-strand components explain these effects. Analysis of neighbouring base pairs reveals that BI/BII transitions of CpG and CpA are easiest within (Y)n(R)n sequences. These can also induce a large vibrational amplitude for TpA steps within the BI conformation.
Bertrand
H
H
Département de Biologie et Pharmacologie Structurales, Institut Gustave Roussy, URA CNRS 147, 39 rue Camille Desmoulins, 94805 Villejuif Cedex, France.
Ha-Duong
T
T
Fermandjian
S
S
Hartmann
B
B
D001482:Base Composition; D001483:Base Sequence; D004247:DNA / Q000737:chemistry*; D018900:Dinucleotide Repeats; D018895:Microsatellite Repeats; D009690:Nucleic Acid Conformation; D013816:Thermodynamics
D004247:DNA