Heat shock treatment releases Penicillium phosphoglycopeptide.
Filtrates of heat (54 degrees) treated day-5 Penicillium fellutanum cultures contained 70 mg of peptidophosphogalactomannan-II; an unheated control contained 30 mg. The polymer contained up to 60 phosphodiesters, and 5-O-beta-D-galactofuranosyl, mannopyranosyl, amino acyl and 2-aminoethanol residues. Its 13C NMR spectrum was nearly identical with that of the control polymer. The major 31P NMR signal was phosphocholine phosphodiester at delta 0.22 ppm: significant phosphodiester signals occurred at delta 1.15, 1.33 and 1.47. Dilute mineral acid released galactofuranosyl residues from the mannan. Signals at delta 1.15-1.47 ppm were associated with molecules of mass less than 3500 and contained galactose, 2-aminoethanol and peptide(s). After this acid treatment, signals at delta 1.0-0.22 remained associated with the mannan. Heat released up to 4-fold more of peptidophosphogalactomannan-III compared with the untreated control; carbohydrate and phosphate content, per mg polymer, were reduced by 4- and 2-fold, respectively. A galactofuranosyl-, phosphoryl- and amino acyl-containing polymer of Mr greater than 14,000 was solubilized by alkali treatment of P. fellutanum cell walls.
Bonetti
S J
SJ
Department of Microbiology and Cell Science, University of Florida, Gainesville 32611-0700, USA.
Groves
S E
SE
Black
B
B
Gander
J E
JE
D002240:Carbohydrate Sequence; D002473:Cell Wall / Q000737:chemistry; D005690:Galactose / Q000031:analogs & derivatives; D006025:Glycosaminoglycans / Q000737:chemistry* / Q000302:isolation & purification; D006358:Hot Temperature; D008351:Mannans; D008969:Molecular Sequence Data; D019906:Nuclear Magnetic Resonance, Biomolecular; D010407:Penicillium / Q000737:chemistry* / Q000254:growth & development
D006025:Glycosaminoglycans; D008351:Mannans; C012990:galactomannan; D005690:Galactose