title: Effect of human neutrophil elastase on tracheal mucociliary transport in anesthetized quails.
abstract: We investigated the effect of human neutrophil elastase (HNE) on tracheal mucociliary transport in anesthetized quails. Topical application of HNE (30-300 microg/kg) to tracheal mucosa dose-dependently decreased mucociliary transport velocity (MCTV). The HNE (300 microg/kg)-induced decrease in MCTV was blocked by ONO-5046 x Na (sodium N-[2-[4-(2,2-dimethylpropionyloxy)phenyl-sulfonylamino]benzo yl]aminoacetate tetrahydrate) (3-30 mg/kg, i.m.), a specific neutrophil elastase inhibitor. Furthermore, we found that HNE increased DNA, fucose and protein contents of tracheal lavages, and the increases were also reverted by ONO-5046 Na. These results indicated that HNE decreased tracheal mucociliary transport, and the decrease may be, at least in part, ascribed to the deterioration of tracheal secretions.
authors.affiliation: Department of Pharmacological Sciences, Faculty of Pharmaceutical Sciences, Kumamoto University, Japan.
authors.forename: S
authors.initials: S
authors.lastname: Tai
authors.forename: H
authors.initials: H
authors.lastname: Kai
authors.forename: T
authors.initials: T
authors.lastname: Kido
authors.forename: Y
authors.initials: Y
authors.lastname: Isohama
authors.forename: K
authors.initials: K
authors.lastname: Takahama
authors.forename: T
authors.initials: T
authors.lastname: Miyata
mesh_terms: D000818:Animals; D001992:Bronchoalveolar Lavage Fluid / Q000737:chemistry; D004247:DNA / Q000032:analysis; D004305:Dose-Response Relationship, Drug; D005643:Fucose / Q000032:analysis; D005998:Glycine / Q000031:analogs & derivatives* / Q000494:pharmacology; D006801:Humans; D019272:Leukocyte Elastase / Q000037:antagonists & inhibitors / Q000494:pharmacology*; D008297:Male; D009079:Mucociliary Clearance / Q000187:drug effects*; D009092:Mucous Membrane / Q000187:drug effects / Q000378:metabolism; D011506:Proteins / Q000032:analysis; D011784:Quail; D015842:Serine Proteinase Inhibitors / Q000494:pharmacology*; D013449:Sulfonamides / Q000494:pharmacology*; D014132:Trachea / Q000187:drug effects* / Q000378:metabolism
chemical_list: D011506:Proteins; D015842:Serine Proteinase Inhibitors; D013449:Sulfonamides; D005643:Fucose; D004247:DNA; C069195:sivelestat; D019272:Leukocyte Elastase; D005998:Glycine