title: Nitric oxide concentration in the gas phase of the gastrointestinal tract in man.
abstract: Nitric oxide (NO) has been implicated in various aspects of physiological regulation in the gastrointestinal tract. Hence, measurement of luminal NO concentrations is of interest for studying physiological and pathophysiological alterations in NO generation; however, at present, no reliable measurement techniques are available. Here we describe novel approaches for measurement of NO concentrations directly in the gas phase of the stomach and colon in healthy subjects and patients. Studies were conducted in young healthy volunteers (n = 13), intensive care patients (n = 8) and patients undergoing gastroscopy (n = 8) or colonoscopy (n = 8). NO concentrations were measured by chemolumininescence detection in air obtained through a nasogastric tube, after inflation into the stomach of a defined volume of air, or directly in the air suctioned from the endoscope. The mean NO concentration obtained from the stomach of healthy volunteers studied under baseline conditions was 18.0 +/- 2.8 (SEM) p.p.m. Day-to-day reproducibility of NO measurements was high. Tube feeding with a nitrite- and nitrate-free feeding solution left gastric NO concentrations unchanged, but standardized bicycle exercise caused an approximately 30% decrease in NO levels. NO concentrations in intensive care patients were approximately 2 log cycles lower than in healthy volunteers. NO levels in the colon were similar to those in the stomach. We have described two readily applicable techniques for direct, uncontaminated measurement of NO concentrations in the lumen of the gastrointestinal tract. Our finding of a striking reduction in gastric NO concentrations in intensive care patients requires further study.
authors.affiliation: Department of Clinical Pharmacology, University of Vienna, Austria.
authors.forename: J
authors.initials: J
authors.lastname: Kastner
authors.forename: F
authors.initials: F
authors.lastname: Pfeffel
authors.forename: A
authors.initials: A
authors.lastname: Rajek
authors.forename: T
authors.initials: T
authors.lastname: Pezawas
authors.forename: M
authors.initials: M
authors.lastname: Hiesmayr
authors.forename: H G
authors.initials: HG
authors.lastname: Eichler
mesh_terms: D000328:Adult; D000368:Aged; D004064:Digestive System / Q000737:chemistry*; D004724:Endoscopy; D005260:Female; D005740:Gases / Q000032:analysis*; D005766:Gastrointestinal Contents / Q000737:chemistry*; D006801:Humans; D006863:Hydrogen-Ion Concentration; D008163:Luminescent Measurements; D008297:Male; D008875:Middle Aged; D009569:Nitric Oxide / Q000032:analysis*
chemical_list: D005740:Gases; D009569:Nitric Oxide