title: Isolation of genomic fragments from polymorphic regions by representational difference analysis.
abstract: Representational difference analysis is an effective technique for isolating the differences between two nearly identical genomes. We have found the technique to be extremely valuable in our analyses of mouse germline mutations. We also present several technical improvements in the procedure that make it more efficient and reliable.
authors.affiliation: Laboratory of Developmental Genetics, Wadsworth Center, David Axelrod Institute for Public Health, Albany, New York 12201-2002, USA.
authors.forename: R A
authors.initials: RA
authors.lastname: Baldocchi
authors.forename: L
authors.initials: L
authors.lastname: Flaherty
mesh_terms: D000818:Animals; D004247:DNA / Q000302:isolation & purification*; D016678:Genome; D006801:Humans; D009693:Nucleic Acid Hybridization / Q000379:methods*; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic
chemical_list: D004247:DNA