title: Chromosomal mapping of genes in the RBCS family in Arabidopsis thaliana.
abstract: In Arabidopsis thaliana, four genes have been identified in the RBCS gene family, one being assigned to subfamily RBCS-A and the other three to subfamily RBCS-B (1B, 2B and 3B). To determine the chromosomal location of these genes, hybridization analysis with CIC YAC high-density filters was carried out for the RBCS-A gene, and CAPS analysis for the three RBCS-B genes, based on the finding that restriction fragment length polymorphism is present in the upstream region of the gene RBCS-3B. The RBCS-A gene was mapped at 100.8 cM from the top of chromosome 1 and the three RBCS-B genes at 62.70 cM from the top of chromosome 5.
authors.affiliation: Laboratory of Plant Cell Technology, Graduate School of Nutritional and Environmental Sciences, University of Shizuoka, Japan. niwa@u-shizuoka-ken.ac.jp
authors.forename: Y
authors.initials: Y
authors.lastname: Niwa
authors.forename: K
authors.initials: K
authors.lastname: Goto
authors.forename: M
authors.initials: M
authors.lastname: Shimizu
authors.forename: H
authors.initials: H
authors.lastname: Kobayashi
mesh_terms: D017360:Arabidopsis / Q000201:enzymology* / Q000235:genetics*; D001483:Base Sequence; D002874:Chromosome Mapping; D018244:Chromosomes, Artificial, Yeast; D017931:DNA Primers; D018744:DNA, Plant / Q000032:analysis / Q000737:chemistry; D004587:Electrophoresis, Agar Gel; D017343:Genes, Plant; D009693:Nucleic Acid Hybridization; D010834:Phytochrome; D012150:Polymorphism, Restriction Fragment Length; D012273:Ribulose-Bisphosphate Carboxylase / Q000235:genetics*
chemical_list: D017931:DNA Primers; D018744:DNA, Plant; D010834:Phytochrome; D012273:Ribulose-Bisphosphate Carboxylase