title: Oligomerization of protegrin-1 in the presence of DPC micelles. A proton high-resolution NMR study.
abstract: Protegrins are members of a family of five Cys-rich naturally occurring cationic antimicrobial peptides. The NMR solution structure of protegrin-1 (PG-1) has been previously determined as a monomeric beta-hairpin both in water and in dimethylsulfoxide solution. Protegrins are bactericidal peptides but their mechanism of action is still unknown. In order to investigate the structural basis of their cytotoxicity, we studied the effect of lipid micelles on the structure of PG-1. The NMR study reported in the present work indicates that PG-1 adopts a dimeric structure when it binds to dodecylphosphocholine micelles. Moreover, the amide proton exchange study suggests the possibility of an association between several dimers.
authors.affiliation: Centre de Biochimie Structurale, CNRS-UMR 9955, INSERM-U414, Université de Montpellier I, Faculté de Pharmacie, France. roume@cbs.univ-montpl.fr
authors.forename: C
authors.initials: C
authors.lastname: Roumestand
authors.forename: V
authors.initials: V
authors.lastname: Louis
authors.forename: A
authors.initials: A
authors.lastname: Aumelas
authors.forename: G
authors.initials: G
authors.lastname: Grassy
authors.forename: B
authors.initials: B
authors.lastname: Calas
authors.forename: A
authors.initials: A
authors.lastname: Chavanieu
mesh_terms: D000890:Anti-Infective Agents / Q000737:chemistry* / Q000378:metabolism; D023181:Antimicrobial Cationic Peptides; D008823:Micelles; D019906:Nuclear Magnetic Resonance, Biomolecular; D009842:Oligopeptides / Q000737:chemistry* / Q000378:metabolism; D010767:Phosphorylcholine / Q000031:analogs & derivatives* / Q000378:metabolism / Q000494:pharmacology; D011487:Protein Conformation; D011506:Proteins / Q000737:chemistry* / Q000378:metabolism; D011522:Protons; D017186:Titrimetry
chemical_list: D000890:Anti-Infective Agents; D023181:Antimicrobial Cationic Peptides; D008823:Micelles; D009842:Oligopeptides; D011506:Proteins; D011522:Protons; C094880:protegrin-1; D010767:Phosphorylcholine; C028810:dodecylphosphocholine