Poly(amino acid)-b-poly(N,N-diethylacrylamide)-b-poly(amino acid) conjugates of well-defined structure.
Carboxy-terminated oligomers of N,N-diethylacrylamide (DEAAm) with low polydispersity were synthesized by group-transfer polymerization. The oligomers were conjugated with the N terminus of epsilon-N-CBZ-protected poly(lysine) and s-CBZ-protected poly(L-cysteine). The resulting conjugates precipitated in a narrow region of lower critical solution temperatures (LCST) around 29 degrees C. The LCST of poly(lysine)-polyDEAAm-poly(lysine) conjugates was pH-independent and independent of the lengths of poly(lysine) segments. Water-soluble nonstoichiometric conjugate-DNA complexes precipitated above the conjugate's LCST. Covalent complexes of human albumin and poly(cysteine)-polyDEAAm-poly(cysteine) conjugates showed temperature-sensitive behaviour in aqueous solutions.
Bromberg
L
L
Department of Materials and Interfaces, Weizmann Institute of Science, Rehovot, Israel. cpbrolev@aol.com
Levin
G
G
D000178:Acrylamides / Q000737:chemistry*; D011232:Chemical Precipitation; D004247:DNA / Q000378:metabolism; D004220:Disulfides / Q000737:chemistry; D004229:Dithiothreitol / Q000494:pharmacology; D006801:Humans; D006863:Hydrogen-Ion Concentration; D009842:Oligopeptides / Q000737:chemistry; D010455:Peptides / Q000737:chemistry*; D011107:Polylysine / Q000737:chemistry; D011108:Polymers / Q000737:chemistry; D012709:Serum Albumin / Q000737:chemistry; D012996:Solutions; D013696:Temperature
D000178:Acrylamides; D004220:Disulfides; D009842:Oligopeptides; D010455:Peptides; D011108:Polymers; D012709:Serum Albumin; D012996:Solutions; D011107:Polylysine; C057713:polycysteine; D004247:DNA; D004229:Dithiothreitol