[HIV infection in a seronegative child diagnosed by isolation of the virus. The significant immunological and epidemiological signs].
HIV-1, subtype F was isolated from a seronegative child aged 2.5 yr. ELISA tests (Behring HIV-1 + 2, Abbott HIV-1 + 2, Wellcozyme HIV-1 Recombinant, Clonatec HIV-1 + 2, Genelavia Mixt), and also rapid tests (Abbott Pack, Serodia) were all negative, although some of them presented borderline reactivities. Western Blot (Cambridge Biotech) revealed an undetermined profile (traces of anti-gp160 plus anti-p24). A new WB test (Sanofi Dg. Pasteur) performed at a higher serum concentration (1/25) revealed a complete antibody profile, despite the very low intensity of bands. A new serum sample prelevated 6 month later was completely negative on all tests used. Both samples, were negatives in WB for HIV2 and HTLVs. A heparinised blood sample was used for the co-cultivation of PBMC and was proven to be positive in the 14th day of culture. The isolated DNA from end-culture cells was subjected to PCR amplifications for Heteroduplex Mobility Assay direct subtyping (primers ES7 and ES8) and for the investigation of genotypic sensitivity to AZT (primers A/NE1). Lymphocyte populations phenotyping revealed leukocytosis (> 15,000/mL) with a predominance of the CD8+ subset CD4/CD8 ratio was < 1. Plasmatic HIV-1 load (measured by bDNA--Chiron) did not reached detectable levels of HIV-1 RNA. p24 Ag assay (EIA-Coulter) revealed a detectable p24 antigenemia only in the first serum sample and only after acid dissociation. So, this patient may present an integrated HIV-1 infection until now "silent".
Apetrei
C
C
Disciplina de Microbiologie, Universitatea de Medicină şi Farmacie Gr. T. Popa, Iaşi.
Grămadă
D
D
Stanciu
V
V
Diculencu
D
D
Pânzaru
C
C
Coman
G
G
Carasievici
E
E
Duca
M
M
D015492:AIDS Serodiagnosis / Q000379:methods / Q000706:statistics & numerical data; D002675:Child, Preschool; D015658:HIV Infections / Q000175:diagnosis* / Q000453:epidemiology / Q000276:immunology / Q000821:virology; D018023:HIV Seronegativity / Q000276:immunology*; D015497:HIV-1 / Q000302:isolation & purification*; D006801:Humans; D008297:Male; D016036:Seroepidemiologic Studies; D019562:Viral Load; D014766:Viremia / Q000175:diagnosis
Infecţie cu HIV la un copil seronegativ diagnosticată prin izolarea virusului. Semnificaţii imunologice şi epidemiologice.