title: New N-glycans in horseradish peroxidase.
abstract: We describe here the structures of several new N-glycan oligosaccharides from horseradish peroxidase (HRP). Glycopeptides from HRP were digested with glycoamidase A (from sweet almond) to release the N-glycans. The oligosaccharides were reductively aminated with 2-aminopyridine, and separated by high-performance liquid chromatography on octadecylsilyl- and amide-silica columns for two-dimensional mapping (Tomiya et al., Anal. Biochem. 171, 73-90, 1988). The N-glycans were also analyzed by electrospray ionization mass spectrometry. Two different lots of HRP showed a considerable difference in their N-glycan composition.
authors.affiliation: GlycoLab, Nakano Vinegar Co., Ltd., Handa City Aichi, Japan.
authors.forename: N
authors.initials: N
authors.lastname: Takahashi
authors.forename: K B
authors.initials: KB
authors.lastname: Lee
authors.forename: H
authors.initials: H
authors.lastname: Nakagawa
authors.forename: Y
authors.initials: Y
authors.lastname: Tsukamoto
authors.forename: K
authors.initials: K
authors.lastname: Masuda
authors.forename: Y C
authors.initials: YC
authors.lastname: Lee
mesh_terms: D000581:Amidohydrolases / Q000378:metabolism; D002240:Carbohydrate Sequence; D002851:Chromatography, High Pressure Liquid; D006020:Glycopeptides / Q000032:analysis; D006735:Horseradish Peroxidase / Q000032:analysis*; D013058:Mass Spectrometry; D008969:Molecular Sequence Data; D009844:Oligosaccharides / Q000032:analysis; D011134:Polysaccharides / Q000032:analysis*
chemical_list: D006020:Glycopeptides; D009844:Oligosaccharides; D011134:Polysaccharides; D006735:Horseradish Peroxidase; D000581:Amidohydrolases