[Differences in the cellular surface of hybrid Escherichia coli K12 bacteria inheriting the rfb a3,4 gene of Shigella flexneri detected using atomic-force microscopy].
The cell surface of E. coli initial parent strain K12 J62 his-, chemotype Ra, and E. coli transductant strain K12 J62 his+, acquiring the capacity for synthesizing primary S-specific side chains of the lipopolysaccharide of S. flexneri O-antigen (group-specific factor 3,4), was studied by the method of atomic force microscopy. The comparative analysis of the images of the genetically linked pair of E.coli strains K12 J62 revealed the presence of essential differences in the topography of the surface structure of the compared bacterial cells, differing in their capacity for synthesizing S. flexneri factor 3,4 represented by repeating chains of L-rhamnose and N-acetyl-D-glucosamine.
Iaminskiĭ
I V
IV
Lomonosov State University, Shemyakin, Russia.
Demin
V V
VV
Bondarenko
V M
VM
D000942:Antigens, Bacterial / Q000235:genetics*; D003433:Crosses, Genetic; D004926:Escherichia coli / Q000235:genetics* / Q000648:ultrastructure*; D005798:Genes, Bacterial / Q000235:genetics*; D018625:Microscopy, Atomic Force / Q000295:instrumentation / Q000379:methods; D019081:O Antigens; D012763:Shigella flexneri / Q000235:genetics* / Q000276:immunology; D013499:Surface Properties
D000942:Antigens, Bacterial; D019081:O Antigens
Razlichiia v kletochnoĭ poverkhnosti gibridnykh bakteriĭ Escherichia coli K12, nasleduiushchikh rfb a3,4 gen Shigella flexneri, vyiavliaemye s pomoshch'iu atomno-silovoĭ mikroskopii.