Regional differences in stratum corneum reactivity to surfactants. Quantitative assessment using the corneosurfametry bioassay.
The skin does not react similarly to the presence of xenobiotics over all anatomic sites. Distinct regional differences have been described for irritancy and percutaneous absorption. The present study assesses the regional variation of stratum corneum reactivity to surfactants using the corneosurfametry bioassay. Stratum corneum was harvested from 6 body sites in 20 young adults. Corneosurfametry was performed using water, 1% SLS and a 5% soap solution. Data show that the best variable to assess regional variability in irritancy is the overall difference in corneosurfametry (ODC), comparing the effect of a given surfactant with water. The dorsal hand and volar forearm were the least reactive, the neck, forehead, back and dorsal foot the most reactive, sites. It is concluded that the corneosurfametry bioassay, through the ODC variable, is a practically noninvasive tool for the evaluation of regional variation in irritancy at the level of the stratum corneum.
Henry
F
F
Belgian SSTC Research Center 5596, Department of Dermatopathology, University of Liège, Belgium.
Goffin
V
V
Maibach
H I
HI
Piérard
G E
GE
D000328:Adult; D000718:Anatomy, Regional; D017449:Dermatitis, Allergic Contact / Q000209:etiology*; D005260:Female; D006801:Humans; D007509:Irritants / Q000009:adverse effects; D008297:Male; D012867:Skin / Q000187:drug effects*; D012869:Skin Absorption; D012882:Skin Tests; D012915:Soaps / Q000009:adverse effects; D013501:Surface-Active Agents / Q000493:pharmacokinetics*
D007509:Irritants; D012915:Soaps; D013501:Surface-Active Agents