A microbore high performance liquid chromatography/electrospray ionization mass spectrometry method for the determination of the phytoestrogens genistein and daidzein in comminuted baby foods and soya flour.
A microbore high performance liquid chromatographic/electrospray/mass spectrometric (HPLC/ESI-MS) method has been developed for the determination of the phytoestrogens daidzein and genistein in soya flours and baby foods. The samples were hydrolysed and extracted with acetonitrile-water prior to analysis. LC was performed on a microbore Primesphere 5C8 column using a water/acetonitrile/acetic acid mobile phase at a flow rate of 60 microliters/min. Atmospheric pressure ionization in the form of pneumatically assisted electrospray mass spectrometry (ESI-MS) was used as the method of detection. The limit of detection was 0.2 mg/ kg for daidzein and 0.7 mg/kg for genistein in the flour and food samples. The method proved both robust and reliable when operated over a long time period (10 days, 463 injections) generating precision data with a coefficient of variation of 4-15%.
Barnes
K A
KA
Ministry of Agriculture, Fisheries and Food, CSL Food Science Laboratory, UK.
Smith
R A
RA
Williams
K
K
Damant
A P
AP
Shepherd
M J
MJ
D002138:Calibration; D002851:Chromatography, High Pressure Liquid; D004968:Estrogens, Non-Steroidal / Q000032:analysis*; D005433:Flour / Q000032:analysis*; D005506:Food Contamination / Q000032:analysis*; D019833:Genistein / Q000032:analysis*; D006868:Hydrolysis; D007225:Infant Food / Q000032:analysis*; D007529:Isoflavones / Q000032:analysis*; D013058:Mass Spectrometry; D011786:Quality Control; D012015:Reference Standards; D013025:Glycine max / Q000737:chemistry*
D004968:Estrogens, Non-Steroidal; D007529:Isoflavones; C004742:daidzein; D019833:Genistein