title: Analysis of the retinoic acid receptor alpha gene as a candidate for the pulmonary adenoma resistance 1 gene.
abstract: The retinoic acid receptor alpha (Rara) gene, which maps in the same region as the pulmonary adenoma resistance (Par1) locus on mouse chromosome 11 (Manenti G et al., Nature Genet 12:455-457, 1996), was tested as a candidate gene for Par1. We report here the analysis of loss of heterozygosity, nucleotide sequence comparison, gene expression, and biochemical activity of the Rara gene from the Mus spretus(Par1/+) and A/J (Par1/-) mouse strains. The two Rara alleles were distinguished by two amino-acid variations but had similar biochemical activity and expression levels, leading to the exclusion of Rara as a candidate Par1 gene.
authors.affiliation: Division of Experimental Oncology A, Istituto Nazionale Tumori, Milan, Italy.
authors.forename: M
authors.initials: M
authors.lastname: Gariboldi
authors.forename: V
authors.initials: V
authors.lastname: Vivat
authors.forename: L
authors.initials: L
authors.lastname: De Gregorio
authors.forename: G
authors.initials: G
authors.lastname: Manenti
authors.forename: E
authors.initials: E
authors.lastname: Chiariello
authors.forename: F S
authors.initials: FS
authors.lastname: Falvella
authors.forename: M A
authors.initials: MA
authors.lastname: Pierotti
authors.forename: T A
authors.initials: TA
authors.lastname: Dragani
mesh_terms: D000236:Adenoma / Q000235:genetics*; D000483:Alleles; D000818:Animals; D014408:Biomarkers, Tumor / Q000235:genetics*; D015972:Gene Expression Regulation, Neoplastic; D016147:Genes, Tumor Suppressor / Q000235:genetics*; D019656:Loss of Heterozygosity; D008175:Lung Neoplasms / Q000235:genetics*; D051379:Mice; D008969:Molecular Sequence Data; D018168:Receptors, Retinoic Acid / Q000235:genetics*; D000072482:Retinoic Acid Receptor alpha
chemical_list: D014408:Biomarkers, Tumor; C000607072:Rara protein, mouse; D018168:Receptors, Retinoic Acid; D000072482:Retinoic Acid Receptor alpha