Polyvinyl alcohol-glutaraldehyde as solid-phase in ELISA for schistosomiasis.
Soluble adult Schistosoma mansoni antigen preparation (SWAP) was covalently fixed onto polyvinyl alcohol-glutaraldehyde discs and an enzyme linked-immunosorbent assay (ELISA) was set up. The best conditions for the assay were established and it was found that small amount of antigen such as 1.5 micrograms was required. A comparison between this procedure and the conventional ELISA was proceeded. A reliable method of antigen immobilization was achieved and the low prices of the employed reagents are economically attractive.
Araújo
A M
AM
Laboratório de Immunopatologia Keizo Asami, Universidade Federal de Pernambuco (UFPE), Recife, PE, Brazil.
Barbosa
G H
GH
Diniz
J R
JR
Malagueño
E
E
Azevedo
W M
WM
de Carvalho Júnior
L B
LB
D000818:Animals; D004797:Enzyme-Linked Immunosorbent Assay; D005976:Glutaral; D006801:Humans; D011142:Polyvinyl Alcohol; D011817:Rabbits; D012552:Schistosomiasis / Q000175:diagnosis*
D011142:Polyvinyl Alcohol; D005976:Glutaral