title: Step-gradient capillary electrochromatography.
abstract: The analytical benefits of using a step-gradient in capillary electrochromatography (CEC) are demonstrated. The application of step-gradient CEC to the analysis of six diuretics of widely differing lipophilicities was evaluated and shown to result in a marked reduction in the analysis time and an improvement in the peak shape for later-eluting lipophilic components. When the step-gradient approach was performed in an automated mode, the retention time RSD for repeated injections was below 1%.
authors.affiliation: Pharmaceutical and Analytical R & D, Astra Charnwood, Loughborough, Leicestershire, UK.
authors.forename: M R
authors.initials: MR
authors.lastname: Euerby
authors.forename: D
authors.initials: D
authors.lastname: Gilligan
authors.forename: C M
authors.initials: CM
authors.lastname: Johnson
authors.forename: K D
authors.initials: KD
authors.lastname: Bartle
mesh_terms: D004232:Diuretics / Q000032:analysis*; D019075:Electrophoresis, Capillary; D005240:Feasibility Studies; D015203:Reproducibility of Results
chemical_list: D004232:Diuretics