Identification of Myo3, a second type-II myosin heavy chain in the fission yeast Schizosaccharomyces pombe.
We cloned the myo3+ gene of Schizosaccharomyces pombe which encodes a type-II myosin heavy chain. myo3 null cells showed a defect in cytokinesis under certain conditions. Overproduction of Myo3 also showed a defect in cytokinesis. Double mutant analysis indicated that Myo3 genetically interacts with Cdc8 tropomyosin and actin. Myo3 may be implicated in cytokinesis and stabilization of F-actin cables. Moreover, the function of Myo2 can be replaced by overexpressed Myo3. We observed a modest synthetic interaction between Myo2 and Myo3. Thus, Myo2 and Myo3 seem to cooperate in the formation of the F-actin ring in S. pombe.
Motegi
F
F
Department of Life Sciences, Graduate School of Arts and Sciences, University of Tokyo, Japan.
Nakano
K
K
Kitayama
C
C
Yamamoto
M
M
Mabuchi
I
I
D000199:Actins / Q000378:metabolism; D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000378:metabolism; D015139:Blotting, Southern; D002352:Carrier Proteins / Q000235:genetics / Q000378:metabolism*; D002455:Cell Division / Q000502:physiology; D002467:Cell Nucleus; D003001:Cloning, Molecular; D005456:Fluorescent Dyes / Q000378:metabolism; D005656:Fungal Proteins / Q000737:chemistry / Q000378:metabolism; D015966:Gene Expression Regulation, Fungal / Q000235:genetics; D008856:Microscopy, Fluorescence; D008969:Molecular Sequence Data; D018995:Myosin Heavy Chains / Q000737:chemistry* / Q000378:metabolism; D024462:Myosin Type II; D024701:Myosin Type V; D009218:Myosins / Q000737:chemistry* / Q000378:metabolism; D010641:Phenotype; D015183:Restriction Mapping; D029701:Saccharomyces cerevisiae Proteins; D012568:Schizosaccharomyces / Q000737:chemistry* / Q000235:genetics; D029702:Schizosaccharomyces pombe Proteins; D017422:Sequence Analysis, DNA; D014335:Tropomyosin / Q000378:metabolism
D000199:Actins; D001426:Bacterial Proteins; D002352:Carrier Proteins; D005456:Fluorescent Dyes; D005656:Fungal Proteins; C077512:MYO2 protein, S cerevisiae; C434211:MYO2 protein, S pombe; C110514:Myp2 protein, S pombe; D029701:Saccharomyces cerevisiae Proteins; D029702:Schizosaccharomyces pombe Proteins; D014335:Tropomyosin; D024462:Myosin Type II; D024701:Myosin Type V; D018995:Myosin Heavy Chains; D009218:Myosins