title: Application of transverse microradiography for measurement of mineral loss by acid erosion.
abstract: This paper describes a novel application of transverse microradiography for the detection and quantification of mineral loss due to acid erosion in thin tooth sections. Sixty-four specimens were randomly divided into eight equal-sized groups and exposed to an orthophosphoric-acid-based erosive fluid (pH = 3) for 0, 0.25, 0.5, 1, 2, 5, 12, or 24 hrs. We made microdensitometric scans separately across both enamel and dentin to derive data for the total mineral loss and the minimum mineral content within the eroded area. We then analyzed specimens using a profilometer to determine (1) the area above a plot of penetration depth against distance and (2) the maximum depth of penetration. Correlation coefficients for comparisons between microradiographic and profilometric data for both enamel and dentin specimens varied between 0.87 and 0.96. Two-sample t tests demonstrated that the microradiographic technique could detect early erosion, i.e., discriminate between erosion times of less than 1 hr. It was concluded that this application of transverse microradiography was a useful and acceptable method for the measurement of early mineral loss in vitro, occurring as a result of acid erosion.
authors.affiliation: Glasgow Dental Hospital and School, Scotland, UK.
authors.forename: A F
authors.initials: AF
authors.lastname: Hall
authors.forename: J P
authors.initials: JP
authors.lastname: Sadler
authors.forename: R
authors.initials: R
authors.lastname: Strang
authors.forename: E
authors.initials: E
authors.lastname: de Josselin de Jong
authors.forename: R H
authors.initials: RH
authors.lastname: Foye
authors.forename: S L
authors.initials: SL
authors.lastname: Creanor
mesh_terms: D015502:Absorptiometry, Photon / Q000379:methods / Q000706:statistics & numerical data; D000134:Acid Etching, Dental; D001641:Bicuspid; D003743:Dental Enamel / Q000000981:diagnostic imaging; D003804:Dentin / Q000000981:diagnostic imaging; D006801:Humans; D066298:In Vitro Techniques; D008852:Microradiography / Q000379:methods* / Q000706:statistics & numerical data; D008963:Molar; D010756:Phosphoric Acids / Q000009:adverse effects*; D011897:Random Allocation; D013997:Time Factors; D017001:Tooth Demineralization / Q000139:chemically induced* / Q000000981:diagnostic imaging*; D014077:Tooth Erosion / Q000139:chemically induced* / Q000000981:diagnostic imaging*
chemical_list: D010756:Phosphoric Acids; C030242:phosphoric acid