Cytochemical detection of endogenous peroxidase in the acinar cells of the hamster submandibular gland.
The presence of endogenous peroxidase activity in the hamster submandibular gland was investigated cytochemically by light and electron microscopy using diaminobenzidine methods. After fixation of tissue with 2% paraformaldehyde-2.5% glutaraldehyde and incubation in a DAB reaction medium containing 0.01% H2O2, the peroxidase reaction product was localized in the nuclear envelope, the cisternae of the endoplasmic reticulum, secretory granules and the Golgi apparatus in both the acinar and granular duct cells of the submandibular gland. This is in contrast to earlier investigators who failed to detect peroxidase activity in acinar cells of the hamster submandibular gland and reported that peroxidase is localized only in the granular duct cells. The discrepancy may be caused by differences in experimental procedures. It is suggested that fixation of tissue with a high concentration of glutaraldehyde and incubation in a DAB reaction medium containing a high concentration of H2O2 inhibits the peroxidase activity of acinar cells in the hamster submandibular gland.
Utsumi
M
M
Department of Anatomy, School of Dentistry, Aichi-Gakuin University, Nagoya, Japan.
Moriguchi
K
K
Ohno
N
N
D015100:3,3'-Diaminobenzidine; D000818:Animals; D006224:Cricetinae; D003594:Cytoplasmic Granules / Q000201:enzymology; D004721:Endoplasmic Reticulum / Q000201:enzymology; D005976:Glutaral; D006056:Golgi Apparatus / Q000201:enzymology; D006861:Hydrogen Peroxide; D008297:Male; D008647:Mesocricetus; D008854:Microscopy, Electron; D009685:Nuclear Envelope / Q000201:enzymology; D009195:Peroxidase / Q000032:analysis*; D013363:Submandibular Gland / Q000201:enzymology* / Q000648:ultrastructure*; D016707:Tissue Fixation
D015100:3,3'-Diaminobenzidine; D006861:Hydrogen Peroxide; D009195:Peroxidase; D005976:Glutaral