[Quality control of stored platelet concentrates by means of flow cytometry].
Storage of single-donor platelets is currently limited to 5 days. During this period, however, numerous morphologic and biochemical changes have been observed. The present study describes increased binding of anti-GMP 140 to stored single-donor platelet concentrates; this reveals a progressive activation process. In contrast, when stored platelets are stimulated with ADP, GMP 140, expression is reduced indicating a diminished release reaction. Additionally, the GP IIb/IIIa receptor complex and its fibrinogen-induced binding site (LIBS1) are reduced by storage time, demonstrating a diminished fibrinogen binding. Further experiments have to clarify whether these changes observed in vitro translate into a reduced hemostatic capacity after transfusion in vivo.
Böck
M
M
Transfusionszentrum, Klinikum Grosshadern, Universität München, Deutschland.
Gawaz
M
M
Heim
M U
MU
Mempel
W
W
D001782:Blood Donors; D001792:Blood Platelets; D001793:Blood Preservation / Q000592:standards*; D005340:Fibrinogen / Q000032:analysis / Q000494:pharmacology; D005434:Flow Cytometry / Q000379:methods*; D006801:Humans; D010974:Platelet Aggregation; D019039:Platelet Glycoprotein GPIIb-IIIa Complex / Q000032:analysis / Q000378:metabolism; D010983:Plateletpheresis / Q000592:standards*; D011786:Quality Control; D013997:Time Factors
D019039:Platelet Glycoprotein GPIIb-IIIa Complex; D005340:Fibrinogen
Qualitätskontrolle gelagerter Thrombozytenkonzentrate mittels Durchflusszytometrie.