New N-glycans in horseradish peroxidase.
We describe here the structures of several new N-glycan oligosaccharides from horseradish peroxidase (HRP). Glycopeptides from HRP were digested with glycoamidase A (from sweet almond) to release the N-glycans. The oligosaccharides were reductively aminated with 2-aminopyridine, and separated by high-performance liquid chromatography on octadecylsilyl- and amide-silica columns for two-dimensional mapping (Tomiya et al., Anal. Biochem. 171, 73-90, 1988). The N-glycans were also analyzed by electrospray ionization mass spectrometry. Two different lots of HRP showed a considerable difference in their N-glycan composition.
Takahashi
N
N
GlycoLab, Nakano Vinegar Co., Ltd., Handa City Aichi, Japan.
Lee
K B
KB
Nakagawa
H
H
Tsukamoto
Y
Y
Masuda
K
K
Lee
Y C
YC
D000581:Amidohydrolases / Q000378:metabolism; D002240:Carbohydrate Sequence; D002851:Chromatography, High Pressure Liquid; D006020:Glycopeptides / Q000032:analysis; D006735:Horseradish Peroxidase / Q000032:analysis*; D013058:Mass Spectrometry; D008969:Molecular Sequence Data; D009844:Oligosaccharides / Q000032:analysis; D011134:Polysaccharides / Q000032:analysis*
D006020:Glycopeptides; D009844:Oligosaccharides; D011134:Polysaccharides; D006735:Horseradish Peroxidase; D000581:Amidohydrolases