title: Diagnosis of chronic camel trypanosomosis by detection of the antibody of trypanosome tyrosine aminotransferase.
abstract: Sera from animals with acute and chronic T. evansi infections were examined directly for trypanosome tyrosine aminotransferase activity and indirectly for the ability of these area from mice and camels with high parasitaemias contained significant levels of trypanosome tyrosine aminotransferase activity. In contrast the chronic sera from both mice and camels did not contain significant tyrosine aminotransferase activity but the chronic sera were able to neutralize the enzyme activity in trypanosome homogenates. In addition to the sera from other pathological conditions did not neutralize the enzyme activity. It is suggested that the inhibitory factor in the chronic sera is antibody. The potential use of the direct enzyme assay, and the indirect neutralization assay as diagnostic tools are discussed. Finally, the use of these assays to distinguish between early (acute) and late (chronic) infections are also suggested.
authors.affiliation: Department of Animal Medicine, Faculty of Veterinary Medicine, Moshtohour, Tukh, Benha.
authors.forename: A
authors.initials: A
authors.lastname: el Sawalhy
authors.forename: S
authors.initials: S
authors.lastname: el-Sherbini
mesh_terms: D000818:Animals; D000913:Antibodies, Protozoan / Q000097:blood*; D002162:Camelus; D051379:Mice; D018512:Parasitemia / Q000097:blood / Q000175:diagnosis / Q000662:veterinary; D014345:Trypanosoma / Q000201:enzymology* / Q000276:immunology; D014352:Trypanosomiasis / Q000097:blood / Q000175:diagnosis / Q000662:veterinary*; D014444:Tyrosine Transaminase / Q000276:immunology*
chemical_list: D000913:Antibodies, Protozoan; D014444:Tyrosine Transaminase