Metabolic changes in red cells in response to adhesion of porcine K88 fimbriated Escherichia coli.
Red cells glycolytic enzymes attached and nonattached to K88+ Escherichia coli were assayed. Hexokinase, glucose-6-phosphate dehydrogenase, 6-phosphogluconate dehydrogenase, pyruvate kinase, glyceraldehyde-3-phosphate dehydrogenase and glutathione reductase activities, were measured. E. coli with K88ab fimbriae, E. coli with K88ac fimbriae, and isolated K88ab fimbriae were investigated for their effect on the above enzymes. Different changes were obtained with K88ab + bacteria compared with K88ac + bacteria. Purified fimbriae gave a third set of responses.
Caloca
M J
MJ
Departamento de Bioquímica y Biología Molecular, Universidad de Leon, Spain.
Suárez
S
S
Soler
J
J
D000818:Animals; D000942:Antigens, Bacterial; D000954:Antigens, Surface / Q000032:analysis; D001422:Bacterial Adhesion; D002645:Chickens; D004798:Enzymes / Q000097:blood; D004912:Erythrocytes / Q000187:drug effects* / Q000201:enzymology*; D004926:Escherichia coli / Q000276:immunology* / Q000472:pathogenicity*; D029968:Escherichia coli Proteins; D036781:Fimbriae Proteins; D010861:Fimbriae, Bacterial / Q000276:immunology; D005954:Glucosephosphate Dehydrogenase / Q000097:blood; D005980:Glutathione Reductase / Q000097:blood; D005987:Glyceraldehyde-3-Phosphate Dehydrogenases / Q000097:blood; D006168:Guinea Pigs; D006593:Hexokinase / Q000097:blood; D007841:Latex Fixation Tests / Q000662:veterinary; D010734:Phosphogluconate Dehydrogenase / Q000097:blood; D011770:Pyruvate Kinase / Q000097:blood; D013552:Swine
D000942:Antigens, Bacterial; D000954:Antigens, Surface; D004798:Enzymes; D029968:Escherichia coli Proteins; C026888:K88 antigen, E coli; D036781:Fimbriae Proteins; D010734:Phosphogluconate Dehydrogenase; D005954:Glucosephosphate Dehydrogenase; D005987:Glyceraldehyde-3-Phosphate Dehydrogenases; D005980:Glutathione Reductase; D006593:Hexokinase; D011770:Pyruvate Kinase