title: Perturbation of CD4+ and CD8+ T-cell repertoires during progression to AIDS and regulation of the CD4+ repertoire during antiviral therapy.
abstract: The T-cell antigen receptor (TCR) repertoire was studied longitudinally by analyzing the varying lengths of the beta chain CDR3 hypervariable region during the course of HIV-1 infection and following combination antiretroviral therapy. Drastic restrictions in CD8+ T-cell repertoire usage were found at all stages of natural progression and persisted during the first six months of treatment. In contrast, significant CD4+ T-cell repertoire perturbations were not found in early stages of infection but correlated with progression to AIDS. Out of ten patients presenting with pretreatment perturbations, normalization of the CD4+ repertoire was observed in eight good responders, but not in two cases of unsuccessful therapy. These results indicate that, besides CD4+ cell count rise, an efficient control of HIV replication may allow qualitative modifications of the CD4+ repertoire balance.
authors.affiliation: Laboratoire d'Immunologie Cellulaire, CERVI, URA CNRS 625, Hôpital Pitié-Salpétrière, Paris, France. guy.gorochov@psl.ap-hop-paris.fr
authors.forename: G
authors.initials: G
authors.lastname: Gorochov
authors.forename: A U
authors.initials: AU
authors.lastname: Neumann
authors.forename: A
authors.initials: A
authors.lastname: Kereveur
authors.forename: C
authors.initials: C
authors.lastname: Parizot
authors.forename: T
authors.initials: T
authors.lastname: Li
authors.forename: C
authors.initials: C
authors.lastname: Katlama
authors.forename: M
authors.initials: M
authors.lastname: Karmochkine
authors.forename: G
authors.initials: G
authors.lastname: Raguin
authors.forename: B
authors.initials: B
authors.lastname: Autran
authors.forename: P
authors.initials: P
authors.lastname: Debré
mesh_terms: D000163:Acquired Immunodeficiency Syndrome / Q000188:drug therapy / Q000276:immunology*; D000328:Adult; D000595:Amino Acid Sequence; D019380:Anti-HIV Agents / Q000627:therapeutic use*; D015496:CD4-Positive T-Lymphocytes / Q000187:drug effects / Q000276:immunology* / Q000821:virology; D018414:CD8-Positive T-Lymphocytes / Q000187:drug effects / Q000276:immunology* / Q000821:virology; D003627:Data Interpretation, Statistical; D018450:Disease Progression; D004359:Drug Therapy, Combination; D006801:Humans; D008297:Male; D008875:Middle Aged; D008969:Molecular Sequence Data; D011948:Receptors, Antigen, T-Cell / Q000187:drug effects / Q000276:immunology*
chemical_list: D019380:Anti-HIV Agents; D011948:Receptors, Antigen, T-Cell