A new polyoxometalate complex inhibits retrovirus encoded reverse transcriptase activity in vitro and in vivo.
We examined the recently synthesized and characterized polyoxometalate compound (NH4)10[Co2Sb2W20 O70(H2O)6] (POM1). The inhibitory potency of POM1 was studied in tissue culture experiments with uninfected and Rous sarcoma virus (RSV)-infected chicken embryo fibroblasts (CEF) in vivo. We measured a considerable decrease in total cellular phosphotyrosine content in treated infected cells in vivo. POM1 treatment of SR-RSV-A infected CEF in vivo resulted in decreased pp60v-src activity, possibly due to a reduced rate of v-src translation caused by the inhibitory effect of POM1 on the RSV encoded reverse transcriptase activity (RT) activity. In further studies we were able to demonstrate the inhibitory effect of this complex on the RT activity of RSV in vitro and in vivo.
Schoeberl
C
C
Department of Experimental Tumor Biology, University of Münster, Badestr. 9, 48149 Münster, Germany.
Boehner
R
R
Krebs
B
B
Mueller
C
C
Barnekow
A
A
D000818:Animals; D001358:Avian Sarcoma Viruses / Q000502:physiology*; D002472:Cell Transformation, Viral / Q000187:drug effects*; D002642:Chick Embryo; D015536:Down-Regulation; D004797:Enzyme-Linked Immunosorbent Assay; D005347:Fibroblasts / Q000201:enzymology* / Q000821:virology; D005434:Flow Cytometry; D015688:Oncogene Protein pp60(v-src) / Q000378:metabolism; D010766:Phosphorylation; D012194:RNA-Directed DNA Polymerase / Q000378:metabolism*; D018894:Reverse Transcriptase Inhibitors / Q000494:pharmacology*; D014162:Transfection; D017973:Tungsten Compounds / Q000494:pharmacology*; D014764:Viral Proteins / Q000032:analysis; D000099294:Polyoxometalates
D015688:Oncogene Protein pp60(v-src); D012194:RNA-Directed DNA Polymerase; D018894:Reverse Transcriptase Inhibitors; D017973:Tungsten Compounds; D014764:Viral Proteins; C102296:polyoxometalate I; D000099294:Polyoxometalates