An expression map from human chromosome 14q24.3.
We have constructed an expression map of chromosome 14q24.3 between markers D14S42 and D14S63. cDNA selection with YACs from 14q24.3 was used to generate expressed sequence tags (ESTs). The localization of ESTs was confirmed on a YAC contig. PCR products of ESTs were used as probes to screen cDNA libraries leading to the isolation of transcripts for known and unknown genes. In total, the expression map contains 7 known genes previously mapped to 14q24.3, 6 cDNA transcripts, and 15 anonymous ESTs. The addition of 21 unique transcribed loci from an approximately 5- to 7-Mb region of chromosome 14q24.3 will facilitate future efforts to identify human disease genes from this region.
Sharma
V
V
Geriatric Research Education Clinical Center, Seattle Veterans' Affairs Medical Center, Washington 98108, USA.
Poorkaj
P
P
Hisama
F
F
Bonnycastle
L
L
Yu
C E
CE
Massa
H
H
Trask
B
B
Clancy
K P
KP
Patterson
D
D
Weissman
S M
SM
Schellenberg
G D
GD
D002874:Chromosome Mapping; D018244:Chromosomes, Artificial, Yeast / Q000235:genetics; D002883:Chromosomes, Human, Pair 14 / Q000235:genetics*; D018076:DNA, Complementary / Q000302:isolation & purification; D015870:Gene Expression; D015723:Gene Library; D005819:Genetic Markers; D006801:Humans; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction
D018076:DNA, Complementary; D005819:Genetic Markers