Double-stranded-RNA-activated protein kinase (PKR) regulates Ca2+ stores in Xenopus oocytes.
Expression of the double-stranded-RNA-dependent protein kinase (PKR) in Xenopus oocytes attenuated Ca2+ entry-dependent membrane currents activated by depletion of Ca2+ stores, whereas expression of a dominant-negative PKR mutant had the opposite effect. These results appeared to be due to perturbation of releasable Ca2+ stores, and not actions of PKR on protein synthesis. PKR may thus have novel protein substrates and cellular functions in Ca2+ storage and signalling.
Thomas
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Department of Biological Chemistry, School of Medicine, University of California, Davis, CA 95616, USA.
Kim
H Y
HY
Morgan
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Hanley
M R
MR
D000818:Animals; D001685:Biological Factors / Q000378:metabolism; D002118:Calcium / Q000378:metabolism*; D015220:Calcium Channels / Q000502:physiology; D000252:Calcium-Transporting ATPases / Q000378:metabolism*; D004594:Electrophysiology; D008845:Microinjections; D009865:Oocytes / Q000378:metabolism*; D014981:Xenopus; D019892:eIF-2 Kinase / Q000378:metabolism*
D001685:Biological Factors; D015220:Calcium Channels; D019892:eIF-2 Kinase; D000252:Calcium-Transporting ATPases; D002118:Calcium