Metabolism of ferulic acid to vanillin. A bacterial gene of the enoyl-SCoA hydratase/isomerase superfamily encodes an enzyme for the hydration and cleavage of a hydroxycinnamic acid SCoA thioester.
A gene encoding a novel enoyl-SCoA hydratase/lyase enzyme for the hydration and nonoxidative cleavage of feruloyl-SCoA to vanillin and acetyl-SCoA was isolated and characterized from a strain of Pseudomonas fluorescens. Feruloyl-SCoA is the CoASH thioester of ferulic acid (4-hydroxy-3-methoxy-trans-cinnamic acid), an abundant constituent of plant cell walls and a degradation product of lignin. The gene was isolated by a combination of mutant complementation and biochemical approaches, and its function was demonstrated by heterologous expression in Escherichia coli under the control of a T7 RNA polymerase promoter. The gene product is a member of the enoyl-SCoA hydratase/isomerase superfamily.
Gasson
M J
MJ
Department of Genetics, Institute of Food Research, Norwich Laboratory, Norwich Research Park, Colney, Norwich NR4 7UA, United Kingdom.
Kitamura
Y
Y
McLauchlan
W R
WR
Narbad
A
A
Parr
A J
AJ
Parsons
E L
EL
Payne
J
J
Rhodes
M J
MJ
Walton
N J
NJ
D000105:Acetyl Coenzyme A / Q000378:metabolism; D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000737:chemistry; D001483:Base Sequence; D001547:Benzaldehydes / Q000378:metabolism*; D003001:Cloning, Molecular; D003373:Coumaric Acids / Q000378:metabolism*; D004746:Enoyl-CoA Hydratase / Q000737:chemistry*; D004926:Escherichia coli / Q000235:genetics; D015870:Gene Expression / Q000235:genetics; D008969:Molecular Sequence Data; D016296:Mutagenesis / Q000235:genetics; D011551:Pseudomonas fluorescens / Q000201:enzymology*; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA
D001426:Bacterial Proteins; D001547:Benzaldehydes; D003373:Coumaric Acids; D000105:Acetyl Coenzyme A; C004999:ferulic acid; C100058:vanillin; D004746:Enoyl-CoA Hydratase