Effects of omega 3 fatty acids on receptor tyrosine kinase and PLC activities in EMT6 cells.
The effects of omega 3 fatty acids and epidermal growth factor (EGF) on the activity of receptor tyrosine kinase (RTK) and phospholipase C (phosphatidylinositol (PI)-specific PLC) were examined in EMT6 cells. The non-omega 3 treated, non-EGF stimulated cells served as controls. Treatment of the EMT6 cells with omega 3 fatty acids resulted in a 62% increase in RTK activity and a 67% increase in PI-specific PLC activity. When EGF was added to incubations for RTK activity, it stimulated the RTK activity 40% in the control cells and 130% in the omega 3-treated cells. When EGF was added to incubations for PI-specific PLC activity, a 54% increase in PI-specific PLC activity was observed in control cells and a 94% increase in the omega 3-treated cells. Thus, treating EMT6 cells with omega 3 fatty acids seems to increase RTK activity and PI-specific PLC activity to a similar extent, but has differential effects on the ability of these enzyme activities to be stimulated by EGF.
Estes
K C
KC
Department of Biochemistry, University of Nevada, Reno 89557, USA.
Rose
B T
BT
Speck
J J
JJ
Nutter
M L
ML
Reitz
R C
RC
D000818:Animals; D004797:Enzyme-Linked Immunosorbent Assay; D004815:Epidermal Growth Factor / Q000494:pharmacology; D015525:Fatty Acids, Omega-3 / Q000494:pharmacology*; D005260:Female; D005395:Fish Oils / Q000494:pharmacology; D008325:Mammary Neoplasms, Experimental / Q000201:enzymology*; D051379:Mice; D043265:Phosphatidylinositol Diacylglycerol-Lyase; D020794:Receptor Protein-Tyrosine Kinases / Q000378:metabolism*; D014407:Tumor Cells, Cultured; D010738:Type C Phospholipases / Q000378:metabolism*
D015525:Fatty Acids, Omega-3; D005395:Fish Oils; D004815:Epidermal Growth Factor; D020794:Receptor Protein-Tyrosine Kinases; D010738:Type C Phospholipases; D043265:Phosphatidylinositol Diacylglycerol-Lyase