Elevated synthesis of biglycan and decorin in an ovine annular lesion model of experimental disc degeneration.
The aim of this study was to extend our earlier observations on the changes that occur in the proteoglycans (PGs) of discs subjected to experimental injury to the annulus fibrosus (AF). We employed the alginate bead culture method to examine the metabolism of the dermatan sulphate (DS) containing PGs by cells derived from different regions of ovine discs that had been subjected to experimental annular injury. This was compared with the metabolism of the DS-PGs by cells isolated from equivalent regions of normal sham-operated discs. Six months after induction of the annular lesion, AF cells isolated from the lesion produced significantly higher levels of decorin and biglycan in alginate bead culture than did cells from equivalent zones of the controls. Decorin and biglycan were identified in culture media samples by immunoblotting, using specific antibodies (6-B-6, LF-96), and also by positive identification of their de-glycosylated core proteins. The core protein of the DS-PGs has been shown to inhibit type I/II collagen fibrillogenesis, to negatively regulate the action of transforming growth factor-beta (TGF-beta) and to diminish cellular proliferation in vitro; events which may be detrimental to tissue repair. The findings are therefore consistent with our previous observation the annular lesions in the avascular inner annulus have no capacity to heal.
Melrose
J
J
Raymond Purves Bone and Joint Research Laboratories, University of Sydney, Royal North Shore Hospital of Sydney, St. Leonards, NSW, Australia.
Ghosh
P
P
Taylor
T K
TK
Vernon-Roberts
B
B
Latham
J
J
Moore
R
R
D000818:Animals; D001345:Autoradiography; D058578:Biglycan; D015153:Blotting, Western; D002455:Cell Division; D002478:Cells, Cultured; D002850:Chromatography, Gel; D058575:Decorin; D003871:Dermatan Sulfate / Q000032:analysis / Q000096:biosynthesis; D004195:Disease Models, Animal; D004591:Electrophoresis, Polyacrylamide Gel; D016326:Extracellular Matrix Proteins; D007403:Intervertebral Disc / Q000737:chemistry / Q000378:metabolism* / Q000473:pathology; D011509:Proteoglycans / Q000032:analysis / Q000096:biosynthesis*; D012756:Sheep; D013128:Spinal Osteophytosis / Q000378:metabolism* / Q000473:pathology
D058578:Biglycan; D058575:Decorin; D016326:Extracellular Matrix Proteins; D011509:Proteoglycans; D003871:Dermatan Sulfate