Localization of the human CXC chemokine subfamily on the long arm of chromosome 4 using radiation hybrids.
All 12 of the human CXC chemokine genes were physically mapped using gene-specific PCR primers and the GenBridge 4 radiation hybrid panel. Nine genes, PF4, PF4V1, GRO1, GCP2, PPBP, IL8, GRO2, GRO3, and SCYB5, were assigned within a 1.8-cR interval of one another on 4q. Two additional genes, MIG and INP10, map within 0.5 cR of each another and 6 cR distal to the above-mentioned group. The final gene, SDF1, is localized on 10q. Phylogenetic analyses of amino acid sequences revealed that SDF1 is the most divergent member and that the physically separated MIG-INP10 pair constitutes a distinct evolutionary lineage.
Modi
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Intramural Research Support Program, SAIC Frederick, National Cancer Institute-Frederick Cancer Research and Development Center, Maryland 21702-1201, USA. modi@ncifcrf.gov
Chen
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D019743:Chemokines, CXC / Q000235:genetics*; D002874:Chromosome Mapping / Q000379:methods*; D002894:Chromosomes, Human, Pair 4; D019143:Evolution, Molecular; D006801:Humans; D006822:Hybrid Cells / Q000528:radiation effects; D010802:Phylogeny; D016133:Polymerase Chain Reaction
D019743:Chemokines, CXC