Lack of protection against avian cholera by vaccination with recombinant P6-like protein from Pasteurella multocida.
The gene encoding the P6-like protein of Pasteurella multocida was cloned in the baculovirus expression system. Baculovirus-expressed recombinant protein was used to parenterally immunize 6-wk-old Nicholas broad-breasted white turkeys. Turkeys developed significant antibody titers to the recombinant protein as measured by enzyme-linked immunosorbent assay. Two weeks after the last immunizing injection, vaccinated turkeys were placed in contact with turkeys infected with P. multocida strain P1059, as were nonvaccinated control birds. No differences occurred in percent mortality between the two groups. We conclude that parenterally administered recombinant P6-like protein does not protect turkeys from avian cholera.
Kasten
R W
RW
Department of Pathology, Microbiology, and Immunology, University of California, Davis 95616, USA.
Wakenell
P S
PS
Ahmad
S
S
Yilma
T D
TD
Hirsh
D C
DC
D000704:Analysis of Variance; D000818:Animals; D000907:Antibodies, Bacterial / Q000032:analysis / Q000276:immunology; D001426:Bacterial Proteins / Q000008:administration & dosage* / Q000276:immunology; D001428:Bacterial Vaccines / Q000008:administration & dosage* / Q000276:immunology; D015169:Colony Count, Microbial; D004797:Enzyme-Linked Immunosorbent Assay / Q000662:veterinary; D005260:Female; D007106:Immune Sera / Q000276:immunology; D010326:Pasteurella Infections / Q000276:immunology / Q000517:prevention & control / Q000662:veterinary*; D016979:Pasteurella multocida / Q000276:immunology* / Q000302:isolation & purification; D011201:Poultry Diseases / Q000276:immunology / Q000517:prevention & control*; D011994:Recombinant Proteins / Q000008:administration & dosage / Q000276:immunology; D014422:Turkeys; D014614:Vaccines, Synthetic / Q000008:administration & dosage* / Q000276:immunology
D000907:Antibodies, Bacterial; D001426:Bacterial Proteins; D001428:Bacterial Vaccines; D007106:Immune Sera; D011994:Recombinant Proteins; D014614:Vaccines, Synthetic