Flow cytometry of mitotic cells.
We have developed a procedure using flow cytometric measurement of a mitosis-specific antigen that may be used to count mitotic cells and sort them from non-mitotic cells. The procedure may also be used in conjunction with measurement of cellular DNA content and of bromodeoxyuridine incorporation into cellular DNA to assign cells to the G1/G0, S, G2, or M phase of the cell cycle.
Anderson
H J
HJ
Department of Biochemistry and Molecular Biology, University of British Columbia, Vancouver, British Columbia, Canada.
de Jong
G
G
Vincent
I
I
Roberge
M
M
D000911:Antibodies, Monoclonal; D000918:Antibody Specificity; D000941:Antigens / Q000032:analysis; D001943:Breast Neoplasms; D002277:Carcinoma; D002469:Cell Separation; D004273:DNA, Neoplasm / Q000032:analysis; D005434:Flow Cytometry / Q000379:methods*; D006801:Humans; D007399:Interphase; D008938:Mitosis; D015739:Nocodazole / Q000494:pharmacology; D010750:Phosphoproteins / Q000032:analysis; D016601:RNA-Binding Proteins / Q000032:analysis; D014407:Tumor Cells, Cultured; D016875:tau Proteins / Q000032:analysis; D000097796:Nucleolin
D000911:Antibodies, Monoclonal; D000941:Antigens; D004273:DNA, Neoplasm; D015739:Nocodazole; D010750:Phosphoproteins; D016601:RNA-Binding Proteins; D016875:tau Proteins; D000097796:Nucleolin