Yeast Clk-1 homologue (Coq7/Cat5) is a mitochondrial protein in coenzyme Q synthesis.
Mutations in the clk-1 gene result in slower development and increased life span in Caenorhabditis elegans. The Saccharomyces cerevisiae homologue COQ7/CAT5 is essential for several metabolic pathways including ubiquinone biosynthesis, respiration, and gluconeogenic gene activation. We show here that Coq7p/Cat5p is a mitochondrial inner membrane protein directly involved in ubiquinone biosynthesis, and that the defect in gluconeogenic gene activation in coq7/cat5 null mutants is a general consequence of a defect in respiration. These results obtained in the yeast model suggest that the effects on development and life span in C. elegans clk-1 mutants may relate to changes in the amount of ubiquinone, an essential electron transport component and a lipid soluble antioxidant.
Jonassen
T
T
Department of Chemistry and Biochemistry and the Molecular Biology Institute, University of California, Los Angeles, Los Angeles, California 90095, USA.
Proft
M
M
Randez-Gil
F
F
Schultz
J R
JR
Marbois
B N
BN
Entian
K D
KD
Clarke
C F
CF
D000595:Amino Acid Sequence; D029742:Caenorhabditis elegans Proteins; D005656:Fungal Proteins / Q000378:metabolism*; D005943:Gluconeogenesis; D005947:Glucose / Q000378:metabolism; D015801:Helminth Proteins / Q000378:metabolism*; D007425:Intracellular Membranes / Q000378:metabolism; D008928:Mitochondria / Q000378:metabolism*; D008969:Molecular Sequence Data; D012441:Saccharomyces cerevisiae / Q000378:metabolism*; D029701:Saccharomyces cerevisiae Proteins; D017386:Sequence Homology, Amino Acid; D014451:Ubiquinone / Q000096:biosynthesis* / Q000235:genetics / Q000378:metabolism*
C097734:CAT5 protein, S cerevisiae; C104339:CLK-1 protein, C elegans; D029742:Caenorhabditis elegans Proteins; D005656:Fungal Proteins; D015801:Helminth Proteins; D029701:Saccharomyces cerevisiae Proteins; D014451:Ubiquinone; D005947:Glucose; C030777:ubiquinone 7