Effects of granulocyte/macrophage colony-stimulating factor on the development and differentiation of CD5-positive macrophages and their potential derivation from a CD5-positive B-cell lineage in mice.
In co-cultures of either the murine pre-B cell line J13, fetal liver cells, or adult peritoneal or bone marrow cells with ST2 mouse bone marrow stromal cells in the presence of granulocyte/macrophage colony-stimulating factor (GM-CSF), the development of CD5+ macrophages was demonstrated by immunohistochemical staining and flow cytometry. Although CD5+ macrophages were not present in the peritoneal cavities of normal mice, approximately 30% of the peritoneal macrophages in viable motheaten (mev/mev) mice, deficient in SHP-1 protein tyrosine phosphatase, expressed cell surface CD5 and B220, markers for B cells. In the mev/mev mice, GM-CSF level in peritoneal fluid was increased significantly. At 5 days after daily intravenous injection with GM-CSF, many CD5+ macrophages appeared in the peritoneal cavity and in omental milky spots of normal mice but fewer in osteopetrosis (op) mutant mice, deficient in macrophage (M)-CSF. These results indicate that GM-CSF, in combination with M-CSF, induces the development and differentiation of CD5+ macrophages in the peritoneal cavity, particularly in the omental milky spots of mice. In the peritoneal cavity of GM-CSF-treated mice, the percentages of hematopoietic progenitor cells doubly positive for CD5 and CD34 or c-kit and of macrophage precursor cells doubly positive for CD5 and ER-MP58 or ER-MP20 were increased significantly during the development of CD5+ macrophages and CD5 B cells, suggesting that CD5+ macrophages and B cells may share a bipotential progenitor in vivo.
Takahashi
K
K
Second Department of Pathology, Kumamoto University School of Medicine, Honjo, Japan.
Miyakawa
K
K
Wynn
A A
AA
Nakayama
K
K
Myint
Y Y
YY
Naito
M
M
Shultz
L D
LD
Tominaga
A
A
Takatsu
K
K
D000818:Animals; D001402:B-Lymphocytes / Q000166:cytology* / Q000378:metabolism*; D001854:Bone Marrow Cells / Q000502:physiology; D018956:CD5 Antigens / Q000378:metabolism*; D002454:Cell Differentiation / Q000187:drug effects / Q000502:physiology; D002460:Cell Line; D016922:Cellular Senescence / Q000187:drug effects / Q000502:physiology; D018920:Coculture Techniques; D016178:Granulocyte-Macrophage Colony-Stimulating Factor / Q000494:pharmacology*; D016130:Immunophenotyping; D008099:Liver / Q000166:cytology / Q000196:embryology; D008264:Macrophages / Q000187:drug effects* / Q000378:metabolism* / Q000648:ultrastructure; D051379:Mice; D008807:Mice, Inbred BALB C; D008809:Mice, Inbred C3H; D010529:Peritoneal Cavity / Q000166:cytology; D017154:Stromal Cells / Q000502:physiology; D013997:Time Factors
D018956:CD5 Antigens; D016178:Granulocyte-Macrophage Colony-Stimulating Factor