title: DNA organization and length polymorphism at the 2L telomeric region of Anopheles gambiae.
abstract: The molecular structure of the telomeric region at the left arm of the second chromosome of the mosquito Anopheles gambiae has been determined in the transformed strain G418 that contains a pUChsneo transgene attached at the 2L chromosome end, and in the Pink eye laboratory strain (PE). Both strains contain the same complex satellite positioned distal to a unique region. FIGE mapping of the telomeric region of the PE strain revealed distinct DNA fragment lengths that segregated with individual chromosomes. Genomic DNA fragments were cloned from the 2L telomeric region, which accounted for about half of 2L chromosomes in the PE population. In all three cases studied, long fragments of different middle repetitive sequences were found attached to the distal ends of the 2L satellite. We propose that random fragments of DNA may be occasionally added during recombination between complex satellite repeats at the chromosome ends.
authors.affiliation: Developmental Biology Center, University of California, Irvine 92697, USA. hbiessma@uci.edu
authors.forename: H
authors.initials: H
authors.lastname: Biessmann
authors.forename: F
authors.initials: F
authors.lastname: Kobeski
authors.forename: M F
authors.initials: MF
authors.lastname: Walter
authors.forename: A
authors.initials: A
authors.lastname: Kasravi
authors.forename: C W
authors.initials: CW
authors.lastname: Roth
mesh_terms: D000818:Animals; D000852:Anopheles / Q000235:genetics*; D001483:Base Sequence; D003001:Cloning, Molecular; D004247:DNA; D004276:DNA, Satellite; D015246:Deoxyribonuclease EcoRI / Q000378:metabolism; D018628:Gene Dosage; D017344:Genes, Insect; D008969:Molecular Sequence Data; D011110:Polymorphism, Genetic; D016615:Telomere
chemical_list: D004276:DNA, Satellite; D004247:DNA; D015246:Deoxyribonuclease EcoRI