Protective effects of carnosine against malondialdehyde-induced toxicity towards cultured rat brain endothelial cells.
Malondialdehyde (MDA) is a deleterious end-product of lipid peroxidation. The naturally-occurring dipeptide carnosine (beta-alanyl-L-histidine) is found in brain and innervated tissues at concentrations up to 20 mM. Recent studies have shown that carnosine can protect proteins against cross-linking mediated by aldehyde-containing sugars and glycolytic intermediates. Here we have investigated whether carnosine is protective against malondialdehyde-induced protein damage and cellular toxicity. The results show that carnosine can (1) protect cultured rat brain endothelial cells against MDA-induced toxicity and (2) inhibit MDA-induced protein modification (formation of cross-links and carbonyl groups).
Hipkiss
A R
AR
Molecular Biology and Biophysics Group, King's College London, Strand, UK.
Preston
J E
JE
Himswoth
D T
DT
Worthington
V C
VC
Abbot
N J
NJ
D000818:Animals; D001921:Brain / Q000187:drug effects / Q000378:metabolism*; D001930:Brain Injuries / Q000139:chemically induced / Q000517:prevention & control*; D002336:Carnosine / Q000494:pharmacology*; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D003459:Crystallins / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D004727:Endothelium / Q000187:drug effects; D005947:Glucose / Q000378:metabolism; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D008315:Malondialdehyde / Q000633:toxicity*; D008928:Mitochondria / Q000378:metabolism; D051381:Rats
D003459:Crystallins; D008315:Malondialdehyde; D002336:Carnosine; D007770:L-Lactate Dehydrogenase; D005947:Glucose