Microbial detection by a glucose biosensor coupled to a microdialysis fibre.
The use of a glucose biosensor coupled to microdialysis sampling in a flow injection analysis system is described to follow the growth of Escherichia coli in a glucose-containing liquid culture medium. The experimental set-up permitted a throughput rate of 25 samples h-1. Growth curves were modelled by a modified Gompertz equation, which permitted the determination of lag time and maximum specific growth rate. The time required to produce an appreciable variation in the biosensor response (minimum detection time, MDT) was determined. A plot of MDT versus microbial concentration was found to be linear in the range 10(6)-10(10) colony forming units (cfu) ml-1. A microbial concentration of 10(6) cfu ml-1 can be detected after about 5 h.
Palmisano
F
F
Dipartimento di Chimica, Università degli Studi, Bari, Italy.
De Santis
A
A
Tantillo
G
G
Volpicella
T
T
Zambonin
P G
PG
D001431:Bacteriological Techniques; D015374:Biosensing Techniques; D004926:Escherichia coli / Q000302:isolation & purification*; D005947:Glucose; D017551:Microdialysis
D005947:Glucose