Structure of oligonucleotides with either a strand break or a bulged nucleotide.
We report NMR and molecular modelling studies on a DNA duplex structure which is composed of three oligonucleotides and mimics a strand break. Although it retains a B form conformation our model suggests that it is kinked at the strand break. In the same sequence with an extra bulged adenosine at the centre for the major species this residue is stacked in the helix and a kink is observed in the model.
Roll
C
C
CEA, Service de Biochimie et Génétique Moléculaire, CEA Saclay, Gif-sur-Yvette, France.
Ketterlé
C
C
Boulard
Y
Y
Fazakerley
G V
GV
D001482:Base Composition; D004249:DNA Damage; D008958:Models, Molecular; D009690:Nucleic Acid Conformation; D009692:Nucleic Acid Heteroduplexes / Q000737:chemistry / Q000378:metabolism; D009841:Oligonucleotides / Q000737:chemistry* / Q000378:metabolism
D009692:Nucleic Acid Heteroduplexes; D009841:Oligonucleotides