Characterization of a molecular clone of HIV type 2 infectious for Macaca nemestrina.
A lambda phage clone containing a full-length HIV-2 provirus, designated HIV-2KR, was obtained from the genomic DNA of Molt4 clone 8 (Molt4/8) lymphoblastic cells infected with the HIV-2PEI2 strain. HIV-2KR is genetically distinct from known HIV-2 isolates, possessing both a unique deletion in the LTR promoter region, and a long rev reading frame. It is replication competent in vitro after transfection into Molt4/8 cells, replicates in a variety of established human T lymphoblastic (Molt-3, Molt4/8, SupT1, H9, C8166) and myelomonocytic (U937) cell lines, and displays prominent cytopathic effects on infection of Molt4/8 cells, reflecting usage of both CCR5 and CXCR4 coreceptors. In addition, HIV-2KR was found to be infectious for human and Macaca nemestrina peripheral blood lymphocytes, and primary human monocyte-macrophage cultures. Intravenous inoculation of cell-free virus into M. nemestrina resulted in infection characterized by transient, low-level viremia and modest temporary decline in CD4 lymphocyte numbers, making HIV-2KR the first HIV-2 molecular clone reported to be infectious for this primate species.
Kraus
G
G
Department of Biology, University of California-San Diego, La Jolla 92093, USA.
Radaelli
A
A
Talbott
R
R
Leavitt
M
M
Schmidt
A
A
Badel
P
P
Bartz
C
C
Morton
W
W
Wong-Staal
F
F
Looney
D J
DJ
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D004195:Disease Models, Animal; D015658:HIV Infections / Q000821:virology*; D015498:HIV-2 / Q000145:classification / Q000235:genetics* / Q000472:pathogenicity; D006801:Humans; D008254:Macaca nemestrina; D008969:Molecular Sequence Data; D010641:Phenotype; D010802:Phylogeny; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid