Expression of the Streptomyces alboniger pur cluster in Streptomyces lividans is dependent on the bldA-encoded tRNALeu.
Streptomyces lividans 1326-9, a bldA+ strain, and its bldA39 mutant derivative J1725 were transformed with a cosmid containing the pur cluster, which determines the puromycin biosynthetic pathway from Streptomyces alboniger. bldA+ transformants produced puromycin in typical amounts, whereas bldA39 transformants did so at drastically decreased levels. Transformation of low producers with the wild-type bldA gene reverted this phenotype to normal production. These data, in addition to the presence of a TTA codon in the amino-terminal coding region of the pur10 and pur6 genes of the pur cluster, suggest that the puromycin biosynthetic pathway is translationally dependent on the bldA gene product, a tRNALeu.
Tercero
J A
JA
Centro de Biología Molecular Severo Ochoa (C.S.I.C./U.A.M.), Universidad Autónoma, Madrid, Spain.
Espinosa
J C
JC
Jiménez
A
A
D000123:Acetyltransferases / Q000378:metabolism; D000581:Amidohydrolases / Q000378:metabolism; D001426:Bacterial Proteins; D015964:Gene Expression Regulation, Bacterial; D005810:Multigene Family; D009154:Mutation; D010641:Phenotype; D011691:Puromycin / Q000096:biosynthesis*; D012329:RNA, Bacterial; D012356:RNA, Transfer, Leu / Q000235:genetics / Q000378:metabolism*; D013302:Streptomyces / Q000235:genetics* / Q000378:metabolism*
D001426:Bacterial Proteins; C107344:BldA tRNA, Streptomyces coelicolor; D012329:RNA, Bacterial; D012356:RNA, Transfer, Leu; D011691:Puromycin; D000123:Acetyltransferases; C045336:puromycin N-acetyltransferase; D000581:Amidohydrolases; C084192:N-acetylpuromycin N-acetylhydrolase