Identification of the fucose synthetase gene in the colanic acid gene cluster of Escherichia coli K-12.
GDP-L-fucose, the substrate for fucosyltransferases for addition of fucose to polysaccharides or glycoproteins in both procaryotes and eucaryotes, is made from GDP-D-mannose. L-Fucose is a component of bacterial surface antigens, including the extracellular polysaccharide colanic acid produced by most Escherichia coli strains. We previously sequenced the E. coli colanic acid gene cluster and identified one of the GDP-L-fucose biosynthetic pathway genes, gmd. We report here the identification of the gene (fcl), located downstream of gmd, encoding the fucose synthetase.
Andrianopoulos
K
K
Department of Microbiology, The University of Sydney, New South Wales, Australia.
Wang
L
L
Reeves
P R
PR
D000595:Amino Acid Sequence; D002238:Carbohydrate Epimerases / Q000235:genetics*; D002352:Carrier Proteins / Q000235:genetics; D004926:Escherichia coli / Q000201:enzymology / Q000235:genetics*; D029968:Escherichia coli Proteins; D005798:Genes, Bacterial; D006154:Guanosine Diphosphate Fucose / Q000096:biosynthesis*; D007658:Ketone Oxidoreductases / Q000235:genetics*; D008969:Molecular Sequence Data; D009097:Multienzyme Complexes / Q000235:genetics*; D005810:Multigene Family; D011134:Polysaccharides / Q000096:biosynthesis* / Q000235:genetics; D011135:Polysaccharides, Bacterial / Q000096:biosynthesis* / Q000235:genetics; D017386:Sequence Homology, Amino Acid
D002352:Carrier Proteins; D029968:Escherichia coli Proteins; D009097:Multienzyme Complexes; D011134:Polysaccharides; D011135:Polysaccharides, Bacterial; C110682:wcaG protein, E coli; D006154:Guanosine Diphosphate Fucose; C004275:colanic acid; C028277:TSTA3 protein, human; D007658:Ketone Oxidoreductases; D002238:Carbohydrate Epimerases