Identification of the chemokine receptor TER1/CCR8 expressed in brain-derived cells and T cells as a new coreceptor for HIV-1 infection.
We have isolated HIV-1 variants that infect brain-derived CD4-positive cells, which are resistant to both macrophage (M)-tropic and T-cell line (T)-tropic HIV-1 strains. It is possible that this brain cell tropism of the HIV-1 variants is determined by the interaction of HIV-1 with a chemokine receptor (CKR) gene. We attempted to detect the expression of the CKR-like genes using degenerate PCR primers. The brain-derived cells expressed a CKR-like gene TER1/CCR8. Human CD4-expressing cells resistant to all HIV-1 strains became susceptible to brain-cell tropic HIV-1 variants after expression of TER1 in these cells, but these cells were still resistant to M-tropic strains or T-tropic IIIB strain. TER1 was expressed in brain-derived cells and human T-cells. These findings suggest that TER1/CCR8 functions as a co-receptor for HIV-1 infection for brain-derived cells as well as T cells.
Jinno
A
A
Department of Hygiene and Virology, Gunma University School of Medicine, Japan.
Shimizu
N
N
Soda
Y
Y
Haraguchi
Y
Y
Kitamura
T
T
Hoshino
H
H
D001921:Brain / Q000166:cytology* / Q000502:physiology; D015496:CD4-Positive T-Lymphocytes / Q000821:virology*; D005786:Gene Expression Regulation / Q000235:genetics; D015497:HIV-1 / Q000235:genetics*; D006801:Humans; D054401:Receptors, CCR8; D019707:Receptors, Chemokine / Q000235:genetics*; D015728:Receptors, HIV / Q000235:genetics*; D014162:Transfection / Q000235:genetics; D018523:Tropism / Q000502:physiology; D014407:Tumor Cells, Cultured
C103330:CCR8 protein, human; D054401:Receptors, CCR8; D019707:Receptors, Chemokine; D015728:Receptors, HIV