Endogenous energy production by mature boar spermatozoa.
When incubated in the absence of exogenous substrates, washed boar spermatozoa maintained a high energy charge potential (ECP) for at least 5 h. Addition of 3-chloro-1-hydroxypropanone, an inhibitor of triosephosphate isomerase and glyceraldehyde 3-phosphate dehydrogenase, at any time caused the ECP to decline and fructose-1,6-bisphosphate, dihydroxyacetone phosphate and glycerol to accumulate. There appear to be two endogenous substrates that are degraded ultimately to produce the triosephosphates which allow the cells to produce lactate for the mitochondrial synthesis of ATP. One substrate generates minor amounts of glycerol 3-phosphate whereas the other substrate degrades to glycerol and may be di-glycerides, or tri-glycerides, or both.
Jones
A R
AR
Department of Biochemistry, University of Sydney, NSW, Australia.
Milmlow
D
D
D000096:Acetone / Q000031:analogs & derivatives; D000255:Adenosine Triphosphate / Q000378:metabolism*; D000818:Animals; D002478:Cells, Cultured; D004099:Dihydroxyacetone Phosphate / Q000378:metabolism; D004734:Energy Metabolism / Q000502:physiology*; D004791:Enzyme Inhibitors / Q000494:pharmacology; D006597:Fructose-Bisphosphatase / Q000378:metabolism; D005987:Glyceraldehyde-3-Phosphate Dehydrogenases / Q000037:antagonists & inhibitors; D005990:Glycerol / Q000378:metabolism; D019344:Lactic Acid / Q000378:metabolism; D008297:Male; D020005:Propanols / Q000494:pharmacology; D013079:Sperm Maturation / Q000502:physiology*; D013094:Spermatozoa / Q000378:metabolism*; D013552:Swine / Q000502:physiology*; D014305:Triose-Phosphate Isomerase / Q000037:antagonists & inhibitors
D004791:Enzyme Inhibitors; D020005:Propanols; D000096:Acetone; C110397:3-chloro-1-propanol; D019344:Lactic Acid; D004099:Dihydroxyacetone Phosphate; D000255:Adenosine Triphosphate; D005987:Glyceraldehyde-3-Phosphate Dehydrogenases; D006597:Fructose-Bisphosphatase; D014305:Triose-Phosphate Isomerase; D005990:Glycerol; C027160:1-hydroxy-3-chloroacetone