Anti-allergic effects and oedema inhibition caused by the extract of Drymis winteri.
OBJECTIVE
To study the acute anti-inflammatory and anti-allergic properties of an extract of D. winteri.


METHODS
Paw oedema induced in rats with various stimuli and anaphylactic shock in mice.


RESULTS
The hydroalcoholic extract (HE) of D. winteri (Winteraceae) (30 to 100 mg/kg, p.o., 1 h prior) inhibited carrageenan (300 micrograms/paw) and dextran (100 micrograms/paw)-induced paw oedema formation in a dose-dependent manner, with mean ID50 values of 49 and < 30 mg/kg, respectively. The HE of D. winteri (30 to 100 mg/kg) also inhibited paw oedema induced by bradykinin (BK) (3 nmol), substance P (SP) (10 nmol) and PAF-acether (PAF) (10 nmol), in a dose-dependent manner, with mean ID50 values of 56, 63, and 58 mg/kg, respectively. However, the HE inhibited the rat paw oedema induced by prostaglandin E2 (PGE2) (10 nmol) (29 +/- 7 and 33 +/- 2% at 60 and 240 min) to a smaller extent, and had no effect on oedema elicited by histamine (100 nmol). In adrenalectomized animals, the inhibition by the HE of D. winteri (100 mg/kg, p.o., 1 h prior) of BK-elicited oedema (3 nmol/paw) was significantly smaller when compared with that observed in control animals. When assessed in rats actively sensitised to ovalbumin (OVO), the oedema caused by OVO (6 micrograms/paw) was significantly inhibited by HE of D. winteri (30 to 100 mg/kg, p.o.), with a mean ID50 of about 65 mg/kg. The HE of D. winteri (100 and 200 mg/kg, p.o.) significantly increased survival rate when assessed in anaphylactic shock in mice actively sensitised to the antigen. The protective effect was long-lasting, being observed for up to 15 h. Dexamethasone, used as positive control (0.5, 1 and 2 mg/kg) produced a long-lasting (up to 24 h) increase in the survival rate of the animals.


CONCLUSIONS
These results confirm and extend our previous studies, and demonstrate the clear oral anti-inflammatory and anti-allergic properties of the active principle(s) present in the barks of D. winteri, thus confirming its reported medicinal use in folk medicine for the management of airway diseases.
Tratsk
K S
KS
Department of Pharmacology, Universidade Federal de Santa Catarina, Florianópolis, Brazil.
Campos
M M
MM
Vaz
Z R
ZR
Filho
V C
VC
Schlemper
V
V
Yunes
R A
RA
Calixto
J B
JB
D000315:Adrenalectomy; D000418:Albumins / Q000276:immunology; D000818:Animals; D018926:Anti-Allergic Agents / Q000627:therapeutic use*; D001938:Brazil; D004305:Dose-Response Relationship, Drug; D004487:Edema / Q000188:drug therapy*; D005528:Foot / Q000473:pathology; D006967:Hypersensitivity / Q000188:drug therapy*; D008297:Male; D051379:Mice; D010936:Plant Extracts / Q000627:therapeutic use*; D010946:Plants, Medicinal / Q000737:chemistry*; D051381:Rats; D017208:Rats, Wistar
D000418:Albumins; D018926:Anti-Allergic Agents; D010936:Plant Extracts