title: High concentration of glucose causes impairment of the function of the glutathione redox cycle in human vascular smooth muscle cells.
abstract: We demonstrated that high glucose reduced H2O2 scavenge activity in human vascular smooth muscle cells. In the cells exposed to high glucose, the intracellular glutathione content decreased, although the NADPH content was unchanged. The rate of uptake of cystine, which is a rate-limiting precursor of the glutathione synthesis, decreased in the high glucose group compared with the control group. These decreases were shown to be dependent on glucose concentration. It was suggested that high glucose causes impairment of the function of the glutathione redox cycle in human vascular smooth muscle cells, resulting in reduced H2O2 scavenge activity.
authors.affiliation: Department of Endocrinology and Metabolism, Institute of Clinical Medicine, University of Tsukuba, Ibaraki, Japan.
authors.forename: Y
authors.initials: Y
authors.lastname: Tachi
authors.forename: Y
authors.initials: Y
authors.lastname: Okuda
authors.forename: C
authors.initials: C
authors.lastname: Bannai
authors.forename: N
authors.initials: N
authors.lastname: Okamura
authors.forename: S
authors.initials: S
authors.lastname: Bannai
authors.forename: K
authors.initials: K
authors.lastname: Yamashita
mesh_terms: D001011:Aorta; D001692:Biological Transport / Q000187:drug effects; D003553:Cystine / Q000378:metabolism; D005947:Glucose / Q000494:pharmacology*; D005978:Glutathione / Q000378:metabolism*; D006801:Humans; D006861:Hydrogen Peroxide / Q000378:metabolism; D007700:Kinetics; D009131:Muscle, Smooth, Vascular / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D009249:NADP / Q000378:metabolism; D010084:Oxidation-Reduction
chemical_list: D003553:Cystine; D009249:NADP; D006861:Hydrogen Peroxide; D005978:Glutathione; D005947:Glucose