Lipopolysaccharide induces apoptosis in a bovine endothelial cell line via a soluble CD14 dependent pathway.
We investigated the mode of bovine endothelial cell death induced by lipopolysaccharide (LPS). Inter-nucleosomal DNA fragmentation was observed indicating apoptotic cell death. Apoptosis was confirmed by examining cell morphology using confocal laser scanning and transmission electron microscopy, which revealed characteristic nuclear condensation in LPS-treated endothelial cells. Apoptosis was blocked by a monoclonal antibody against CD14, suggesting that LPS triggers apoptosis via a soluble CD14 (sCD14) dependent mechanism.
Frey
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Biotechnology Laboratory, University of British Columbia, 6174 University Boulevard, Vancouver, B.C., V6T 1Z3, Canada.
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D000818:Animals; D000911:Antibodies, Monoclonal; D017209:Apoptosis; D002417:Cattle; D002460:Cell Line; D053938:DNA Fragmentation; D004305:Dose-Response Relationship, Drug; D004730:Endothelium, Vascular / Q000166:cytology* / Q000276:immunology* / Q000648:ultrastructure; D018950:Lipopolysaccharide Receptors / Q000276:immunology / Q000502:physiology*; D008070:Lipopolysaccharides / Q000008:administration & dosage / Q000494:pharmacology*; D018613:Microscopy, Confocal; D008854:Microscopy, Electron; D011651:Pulmonary Artery / Q000166:cytology
D000911:Antibodies, Monoclonal; D018950:Lipopolysaccharide Receptors; D008070:Lipopolysaccharides