title: Cloning, sequencing, and transgenic expression of Podospora curvicolla and Sordaria macrospora eEF1A genes: relationship between cytosolic translation and longevity in filamentous fungi.
abstract: We have cloned and sequenced the gene encoding the translation elongation factor eEF1A from two filamentous fungi, Podospora curvicolla and Sordaria macrospora. These fungi are close relatives of Podospora anserina and also show senescence syndromes. Comparison of the sequences of the deduced proteins with that of P. anserina reveals that the three proteins differ in several positions. Replacement of the P. anserina gene by either of the two exogenous genes does not entail any modification in P. anserina physiology; the longevity of the fungus is not affected. No alteration of in vivo translational accuracy was detected; however, the exogenous proteins nonetheless promoted a modification of the resistance to the aminoglycoside antibiotic paromomycin. These data suggest that optimization of life span between these closely related fungi has likely not been performed during evolution through modifications of eEF1A activity, despite the fact that mutations in this factor can drastically affect longevity.
authors.affiliation: Institut de Génétique et Microbiologie, CNRS URA 2225, Bât. 400 Université de Paris Sud, Orsay cedex, 91405, France.
authors.forename: B
authors.initials: B
authors.lastname: Gagny
authors.forename: M
authors.initials: M
authors.lastname: Rossignol
authors.forename: P
authors.initials: P
authors.lastname: Silar
mesh_terms: D000595:Amino Acid Sequence; D001203:Ascomycota / Q000502:physiology*; D003001:Cloning, Molecular; D003600:Cytosol / Q000378:metabolism; D005800:Genes, Fungal; D008969:Molecular Sequence Data; D020648:Peptide Elongation Factor 1; D010445:Peptide Elongation Factors / Q000235:genetics*; D014176:Protein Biosynthesis; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D013045:Species Specificity
chemical_list: D020648:Peptide Elongation Factor 1; D010445:Peptide Elongation Factors