[p16INK4/CDKN2 gene deletions and mutations in non-small cell lung cancers].
OBJECTIVE
To investigate the genomic status of cyclindependent kinase-4 inhibitor, p16 INK4/CDKN2 gene in primary non-small cell lung cancers.


METHODS
p16 gene was analysed by duplex PCR, PCR-SSCP, and sequencing in 31 primary non-small cell lung cancer (NSCLC) tissues.


RESULTS
Gene deletions were found in three samples (3/31), one case had whole p16 gene deletion and the other two had deletion in exon 1 and in exon 2 respectively. Two cases of point mutations (2/30) in the gene had been found, one in exon 1 and another one in exon 2. In the 5 cases of p16 gene abnormal samples, 4 cases (two cases of deletions and two cases of mutations) were non-small cell lung cancers with lymphnode metastasis (4/20).


CONCLUSIONS
These results indicate that inactivation of p16 gene is associated with some NSCLC (5/31), and probably be a late event in NSCLC carcinogenesis.
Wang
G
G
Department of Medical Genetics, Sun Yat-sen University of Medical Sciences, Guangzhou, 510089 P. R. China.
Du
C
C
Lin
Q
Q
D000328:Adult; D002289:Carcinoma, Non-Small-Cell Lung / Q000235:genetics*; D005260:Female; D017353:Gene Deletion; D019942:Genes, p16; D006801:Humans; D008175:Lung Neoplasms / Q000235:genetics*; D008297:Male; D008875:Middle Aged; D009154:Mutation