title: Seroreactivity to HIV-1 V3 subtypes A to H peptides of Argentinian HIV-positive sera.
abstract: Serologic assays could be useful for determining circulating subtypes in different geographic regions. A total of 175 serum samples from the same number of Argentinian HIV-infected patients from Buenos Aires and Rosario were tested against a panel of peptides representing V3 consensus subtypes A through H. A V3 peptide enzyme immunoassay was used for screening the sera. Most sera were reactive with peptides representing subtypes B (58.28%), F (13.14%), and A (8.57%). Cross-reactivity between the remainder of the peptides was observed. Genotypes of eight patients from Rosario were determined and compared with serotyping. Results showed that seven of eight genotyped patients reacted with their respective consensus B peptide and one reacted with consensus B and F. V3 peptide serology proved to be useful for determining HIV-1 clades circulating in Argentina.
authors.affiliation: Department of Microbiology, University of Buenos Aires School of Medicine, Argentina.
authors.forename: C
authors.initials: C
authors.lastname: Devito
authors.forename: M
authors.initials: M
authors.lastname: Levi
authors.forename: J
authors.initials: J
authors.lastname: Hinkula
authors.forename: R D
authors.initials: RD
authors.lastname: Fernandez Medina
authors.forename: O
authors.initials: O
authors.lastname: Libonatti
authors.forename: H
authors.initials: H
authors.lastname: Wigzell
mesh_terms: D000595:Amino Acid Sequence; D001118:Argentina / Q000453:epidemiology; D015483:HIV Antibodies / Q000097:blood / Q000145:classification*; D015699:HIV Envelope Protein gp120 / Q000145:classification* / Q000276:immunology; D006679:HIV Seropositivity / Q000453:epidemiology / Q000276:immunology / Q000821:virology*; D015497:HIV-1 / Q000145:classification* / Q000276:immunology; D006801:Humans; D007074:Immunoglobulin G / Q000276:immunology; D008969:Molecular Sequence Data; D010446:Peptide Fragments / Q000145:classification* / Q000276:immunology; D015819:Substance Abuse, Intravenous
chemical_list: D015483:HIV Antibodies; D015699:HIV Envelope Protein gp120; C059762:HIV envelope protein gp120 (305-321); D007074:Immunoglobulin G; D010446:Peptide Fragments