Cloning and expression of a human/mouse Polycomb group gene, ENX-2/Enx-2.
The Drosophila Polycomb group (Pc-G) genes encode transcriptional factors involved in development. Little is known about members of the vertebrate Pc-G genes. In this study, we have isolated a cDNA encoding a human Pc-G protein and the mouse equivalent. The human and mouse genes, which were named ENX-2 and Enx-2, encode 702 and 750 amino acids, respectively. ENX-2/Enx-2 protein exhibits a high homology (53-55% identity) to Drosophila Enhancer of zeste [E(z)] protein belonging to the Pc-G. The expression of Enx-2 was observed in mouse kidney, adrenal gland, testis and brain at high levels by Northern blot analysis. A cell line of mouse neuroblastoma, Neuro-2a, also expresses Enx-2 mRNA and its level is elevated by induction of neuronal differentiation of the cell.
Ogawa
M
M
Department of Anatomy, Keio University School of Medicine, Tokyo, Japan.
Hiraoka
Y
Y
Taniguchi
K
K
Aiso
S
S
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002417:Cattle; D003001:Cloning, Molecular; D018076:DNA, Complementary; D015870:Gene Expression; D000882:Haplorhini; D006801:Humans; D051379:Mice; D008969:Molecular Sequence Data; D063146:Polycomb-Group Proteins; D011817:Rabbits; D051381:Rats; D012097:Repressor Proteins / Q000096:biosynthesis / Q000235:genetics* / Q000378:metabolism; D017386:Sequence Homology, Amino Acid; D013552:Swine; D014018:Tissue Distribution; D014407:Tumor Cells, Cultured
D018076:DNA, Complementary; D063146:Polycomb-Group Proteins; D012097:Repressor Proteins