[Regulation of Staphylococcus aureus capsular polysaccharide type 5: in vitro and in vivo inhibition by CO2].
Staphylococcus aureus capsular polysaccharide type 5 (CP5) expression was investigated in lung tissue and nasal polyps of two cystic fibrosis (CF) patients, in rats and in vitro using ELISA and immunofluorescence. In CF tissues, S. aureus expressed protein A and teichoic acid but only 1-5% of cells expressed CP5. When rats were challenged with CP5-positive S. aureus in the granuloma pouch model, only 1-5% CP5-positive cells were detectable in pouch exsudates. CF and pouch isolates, however, re-expressed CP5 (70-90% of cells) when grown in vitro with air. Addition of 1% CO2 or more to air or to O2/N2 gas mixtures reduced CP5 expression significantly (p < 0.001) in a dose-dependent manner (1-6% CP5-positive cells). The results show that S. aureus does not produce CP5 in CF airways and in rat granuloma pouches and that CO2 is an environmental signal which regulates CP5 expression.
Herbert
S
S
Abteilung für Allgemeine Hygiene und Umwelthygiene, Universität Tübingen, Deutschland.
Worlitzsch
D
D
Dassy
B
B
Boutonnier
A
A
Fournier
J M
JM
Bellon
G
G
Dalhoff
A
A
Döring
G
G
D000328:Adult; D000818:Animals; D016667:Bacterial Capsules; D002245:Carbon Dioxide / Q000494:pharmacology*; D002648:Child; D003550:Cystic Fibrosis / Q000382:microbiology*; D004195:Disease Models, Animal; D005260:Female; D006801:Humans; D066298:In Vitro Techniques; D008168:Lung / Q000382:microbiology; D008297:Male; D009297:Nasal Mucosa / Q000382:microbiology; D011127:Polyps / Q000382:microbiology; D011135:Polysaccharides, Bacterial / Q000302:isolation & purification*; D051381:Rats; D013203:Staphylococcal Infections / Q000150:complications* / Q000382:microbiology; D013211:Staphylococcus aureus / Q000302:isolation & purification*
D011135:Polysaccharides, Bacterial; D002245:Carbon Dioxide
Regulation des Staphylococcus aureus-Kapselpolysaccharids vom Typ 5: Hemmung durch CO2 in vitro und in vivo.