Expression of murine novel zinc finger proteins highly homologous to Drosophila ovo gene product in testis.
We have cloned two isoforms of cDNAs encoding novel zinc finger proteins. One form encodes a 274-amino acid protein containing an acidic amino acid and serine-rich domain and a zinc finger domain which shows high sequence homology to that of Drosophila Ovo protein. The other form encodes a 179-amino acid protein containing only the zinc finger domain. Expression of both proteins possessing an antigenic epitope in COS cells revealed that they are localized in the nucleus. The 1.3-kbp mRNAs are predominantly expressed in testis, and the expression increases from 3 weeks postnatal, implying that these proteins may play important roles in the development of the testes.
Masu
Y
Y
Department of Medical Chemistry, Kansai Medical University, Moriguchi, Osaka, Japan.
Ikeda
S
S
Okuda-Ashitaka
E
E
Sato
E
E
Ito
S
S
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D015152:Blotting, Northern; D019556:COS Cells; D018076:DNA, Complementary; D004268:DNA-Binding Proteins / Q000737:chemistry / Q000235:genetics*; D004330:Drosophila; D029721:Drosophila Proteins; D018507:Gene Expression Regulation, Developmental; D008297:Male; D051379:Mice; D008969:Molecular Sequence Data; D017386:Sequence Homology, Amino Acid; D013737:Testis / Q000378:metabolism; D014157:Transcription Factors / Q000737:chemistry / Q000235:genetics*; D016335:Zinc Fingers
D018076:DNA, Complementary; D004268:DNA-Binding Proteins; D029721:Drosophila Proteins; C490318:Ovo1 protein, mouse; D014157:Transcription Factors; C089512:ovo protein, Drosophila