title: Effects of six weeks of chronic ethanol administration on lactic acid accumulation and high energy phosphate levels after experimental brain injury in rats.
abstract: The effects of 6 weeks of chronic ethanol administration on the lateral fluid percussion (FP) brain injury-induced regional accumulation of lactate and on the levels of total high-energy phosphates were examined in rats. In both the chronic ethanol diet (ethanol diet) and pair-fed isocaloric sucrose control diet (control diet) groups, tissue concentrations of lactate were elevated in the cortices and hippocampi of both the ipsilateral and contralateral hemispheres at 5 min after brain injury. In both diet groups, concentrations of lactate were elevated only in the injured left cortex and the ipsilateral hippocampus at 20 min after FP brain injury. No significant differences were found in the levels of lactate in the cortices and hippocampi of sham animals and brain-injured animals between the ethanol and control diet groups at 5 min and 20 min after injury. In the ethanol and control diet groups, tissue concentrations of total high-energy phosphates (ATP + PCr) were not affected in the cortices and hippocampi at 5 min and 20 min after lateral FP brain injury. No significant differences were found in the levels of total high-energy phosphates in the cortices and hippocampi of the sham and brain-injured animals between the ethanol and control diet groups at 5 min and 20 min after injury. Histologic studies revealed a similar extent of damage in the cortex and in the CA3 region of the ipsilateral hippocampus in both diet groups at 14 days after lateral FP brain injury. These findings suggest that 6 weeks of chronic ethanol administration does not alter brain injury-induced accumulation of lactate, levels of total high energy phosphates, and extent of morphological damage.
authors.affiliation: Department of Surgery, University of Kentucky Chandler Medical Center, University of Kentucky, Lexington 40536-0084, USA.
authors.forename: R M
authors.initials: RM
authors.lastname: Prasad
authors.forename: A
authors.initials: A
authors.lastname: Laabich
authors.forename: H S
authors.initials: HS
authors.lastname: Dhillon
authors.forename: L
authors.initials: L
authors.lastname: Zhang
authors.forename: A
authors.initials: A
authors.lastname: Maki
authors.forename: W J
authors.initials: WJ
authors.lastname: Clerici
authors.forename: R
authors.initials: R
authors.lastname: Hicks
authors.forename: J
authors.initials: J
authors.lastname: Butcher
authors.forename: S
authors.initials: S
authors.lastname: Barron
mesh_terms: D000255:Adenosine Triphosphate / Q000378:metabolism*; D000818:Animals; D001835:Body Weight / Q000187:drug effects; D001921:Brain / Q000473:pathology; D001930:Brain Injuries / Q000097:blood / Q000378:metabolism* / Q000473:pathology; D002492:Central Nervous System Depressants / Q000097:blood / Q000494:pharmacology*; D000431:Ethanol / Q000097:blood / Q000494:pharmacology*; D019344:Lactic Acid / Q000378:metabolism*; D008297:Male; D010725:Phosphocreatine / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D002492:Central Nervous System Depressants; D010725:Phosphocreatine; D019344:Lactic Acid; D000431:Ethanol; D000255:Adenosine Triphosphate