title: Synthesis and binding properties of oligodeoxynucleotides containing phenylphosphon(othio)ate linkages.
abstract: A method for the synthesis of chimeric oligodeoxynucleotides comprised of phosphodiester and phenylphosphonate [3'-O-P(= O)(C6H5)-O-5'] or phenylphosphono-thioate [3'-O-P(= S)(C6H5)-O-5'] linkages has been developed. Synthesis was performed using suitably protected nucleoside phenylphosphonamidites as building blocks following an adjusted solid-phase phosphoramidite synthesis protocol. The new oligodeoxy-nucleotide analogues were characterized by electrospray ionization- and matrix-assisted laser desorption mass spectrometry, as well as by 31P NMR spectroscopy. Additionally, their binding properties to complementary oligodeoxynucleotides has been studied.
authors.affiliation: Hoechst Aktiengesellschaft, Frankfurt am Main, Germany.
authors.forename: M
authors.initials: M
authors.lastname: Mag
authors.forename: J
authors.initials: J
authors.lastname: Muth
authors.forename: K
authors.initials: K
authors.lastname: Jahn
authors.forename: A
authors.initials: A
authors.lastname: Peyman
authors.forename: G
authors.initials: G
authors.lastname: Kretzschmar
authors.forename: J W
authors.initials: JW
authors.lastname: Engels
authors.forename: E
authors.initials: E
authors.lastname: Uhlmann
mesh_terms: D002851:Chromatography, High Pressure Liquid; D009682:Magnetic Resonance Spectroscopy; D008956:Models, Chemical; D009838:Oligodeoxyribonucleotides / Q000138:chemical synthesis* / Q000378:metabolism; D009946:Organothiophosphorus Compounds / Q000138:chemical synthesis* / Q000378:metabolism; D016339:Spectrometry, Mass, Fast Atom Bombardment
chemical_list: D009838:Oligodeoxyribonucleotides; D009946:Organothiophosphorus Compounds