title: Structural and immunochemical characterization of beta-1,2-linked mannobiosyl phosphate residue in the cell wall mannan of Candida glabrata.
abstract: A mannan of Candida glabrata IFO 0622 digested by Arthrobacter exo-alpha-mannosidase and a beta-1,2-linked mannobiose obtained from the parent mannan by acid treatment was analyzed using 13C nuclear magnetic resonance spectroscopy. The results show that the beta-1, 2-linked mannobiosyl residue is esterified to a phosphate group through position C-1 in the alpha-configuration, Manbeta1- 2Manalpha1-HPO3-. The results of immunochemical assays of these mannans using the commercial antigenic factor sera of the genus Candida (Candida Check, Iatron) indicate that the main recognition site of serum no. 6 in this kit is the mannotetraosyl side-chain Manbeta1-2Manalpha1- 2Manalpha1-2Man in C. glabrata mannan and also suggest that the phosphate-containing unit (such as Manbeta1- 2Manalpha1-HPO3- in this mannan) behaves as one of the antigenic determinants of serum no. 6, but not of serum no. 5. Therefore, the present and previous findings indicate that serum no. 5 recognizes relatively longer beta-1,2-linked oligomannosyl side-chains, Manbeta1-[2Manbeta1-]n 2Man (n = 1-6), attached to the phosphate groups previously observed in the cell wall mannans of Candida albicans, Candida stellatoidea, and Candida tropicalis.
authors.affiliation: Second Department of Hygienic Chemistry, Tohoku College of Pharmacy, 4-4-1 Komatsushima, Aoba-ku, Sendai, Miyagi 981, Japan.
authors.forename: H
authors.initials: H
authors.lastname: Kobayashi
authors.forename: H
authors.initials: H
authors.lastname: Oyamada
authors.forename: N
authors.initials: N
authors.lastname: Iwadate
authors.forename: H
authors.initials: H
authors.lastname: Suzuki
authors.forename: H
authors.initials: H
authors.lastname: Mitobe
authors.forename: K
authors.initials: K
authors.lastname: Takahashi
authors.forename: N
authors.initials: N
authors.lastname: Shibata
authors.forename: S
authors.initials: S
authors.lastname: Suzuki
authors.forename: Y
authors.initials: Y
authors.lastname: Okawa
mesh_terms: D000371:Agglutination; D002175:Candida / Q000737:chemistry*; D002241:Carbohydrates / Q000032:analysis; D002473:Cell Wall / Q000737:chemistry*; D004797:Enzyme-Linked Immunosorbent Assay; D005656:Fungal Proteins / Q000032:analysis; D009682:Magnetic Resonance Spectroscopy; D008351:Mannans / Q000737:chemistry*; D008358:Mannose / Q000032:analysis; D008565:Membrane Proteins / Q000032:analysis; D010710:Phosphates / Q000032:analysis
chemical_list: D002241:Carbohydrates; D005656:Fungal Proteins; D008351:Mannans; D008565:Membrane Proteins; D010710:Phosphates; D008358:Mannose