Synthesis and binding properties of oligodeoxynucleotides containing phenylphosphon(othio)ate linkages.
A method for the synthesis of chimeric oligodeoxynucleotides comprised of phosphodiester and phenylphosphonate [3'-O-P(= O)(C6H5)-O-5'] or phenylphosphono-thioate [3'-O-P(= S)(C6H5)-O-5'] linkages has been developed. Synthesis was performed using suitably protected nucleoside phenylphosphonamidites as building blocks following an adjusted solid-phase phosphoramidite synthesis protocol. The new oligodeoxy-nucleotide analogues were characterized by electrospray ionization- and matrix-assisted laser desorption mass spectrometry, as well as by 31P NMR spectroscopy. Additionally, their binding properties to complementary oligodeoxynucleotides has been studied.
Mag
M
M
Hoechst Aktiengesellschaft, Frankfurt am Main, Germany.
Muth
J
J
Jahn
K
K
Peyman
A
A
Kretzschmar
G
G
Engels
J W
JW
Uhlmann
E
E
D002851:Chromatography, High Pressure Liquid; D009682:Magnetic Resonance Spectroscopy; D008956:Models, Chemical; D009838:Oligodeoxyribonucleotides / Q000138:chemical synthesis* / Q000378:metabolism; D009946:Organothiophosphorus Compounds / Q000138:chemical synthesis* / Q000378:metabolism; D016339:Spectrometry, Mass, Fast Atom Bombardment
D009838:Oligodeoxyribonucleotides; D009946:Organothiophosphorus Compounds