Intracerebral injection of interferon-gamma inhibits the astrocyte proliferation following the brain injury in the 6-day-old rat.
The present study examines the influence of interferon-gamma (IFN-gamma) on the astrocyte proliferation in the rat brain injured within the early period of postnatal development. Six-day-old male rats received a lesion in the left cerebral hemisphere and a single injection of recombinant rat IFN-gamma into the lesion cavity. One or 2 days after the injury the rats were injected with 3H-thymidine. Brain sections were immunostained for glial fibrillary acidic protein (GFAP), subjected to autoradiography, and examined microscopically to record proliferating GFAP-immunopositive astrocytes labeled with 3H-thymidine. In the IFN-gamma-injected rats, a statistically significant decrease in the intensity of reactive astrocyte proliferation was revealed. On day 1 after injury the intensity of astrocyte proliferation showed dose-dependent changes. Relations between the astrocyte reactivity and multiple factors existing in the injured and IFN-gamma-injected brain are discussed. The results represent the first in vivo evidence of a dose-dependent action of IFN-gamma on the astrocyte proliferation in response to injury.
Pawliński
R
R
Department of Neuroanatomy, Institute of Zoology, Jagiellonian University, Kraków, Poland.
Janeczko
K
K
D000818:Animals; D001253:Astrocytes / Q000187:drug effects* / Q000473:pathology; D001921:Brain; D001930:Brain Injuries / Q000188:drug therapy* / Q000473:pathology; D002455:Cell Division / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D007267:Injections; D007371:Interferon-gamma / Q000494:pharmacology*; D008297:Male; D051381:Rats; D017208:Rats, Wistar; D011994:Recombinant Proteins
D011994:Recombinant Proteins; D007371:Interferon-gamma