Incidence of rat-soluble placental factors on IgE and IgG2a synthesis.
OBJECTIVE
The in vivo effect of soluble factors present in placental culture supernatants (PCSs) on the synthesis of rat immunoglobulin E (IgE) and IgG2a isotypes was investigated.


METHODS
Batches of Wistar SPF rats immunized with a 10-microgram dose of ovalbumin and Al(OH)3 were used: group I, consisted of virgin rats; group II, virgin females injected simultaneously with PCSs; and group III, pregnant females. As controls, nonimmunized batches were included. Serum samples were collected at days 0 (basal) and 10 after antigen challenge, determining levels of total and specific antiovalbumin of both IgE and IgG2a by enzyme-linked immunoadsorbent assay (ELISA).


RESULTS
In vivo and at least at the doses administered, PCSs exert an inhibitory effect on the synthesis of specific and total anti-ovalbumin IgE during the course of immune response to such challenge. However, PCSs did not modify serum values of total and specific IgG2a.


CONCLUSIONS
These results suggest that PCSs exert selective influence on the synthesis of diverse immunoglobulin isotypes during immune response, through the balance of cytokines synthesized by placental cells.
Gentile
T
T
Instituto de Estudios de la Inmunidad Humoral (CONICET-UBA), Departamento de Microbiología, Facultad de Farmacia y Bioquímica, Universidad de Buenos Aires, Argentina.
Dokmetjian
J
J
Garrido
D
D
Margni
R A
RA
D000818:Animals; D000906:Antibodies / Q000097:blood; D046508:Culture Techniques; D005260:Female; D007073:Immunoglobulin E / Q000096:biosynthesis* / Q000097:blood; D007074:Immunoglobulin G / Q000096:biosynthesis* / Q000097:blood; D007132:Immunoglobulin Isotypes / Q000096:biosynthesis* / Q000097:blood; D010047:Ovalbumin / Q000276:immunology; D010920:Placenta / Q000187:drug effects* / Q000378:metabolism*; D010924:Placental Extracts / Q000494:pharmacology*; D011247:Pregnancy; D051381:Rats; D017208:Rats, Wistar; D012995:Solubility
D000906:Antibodies; D007074:Immunoglobulin G; D007132:Immunoglobulin Isotypes; D010924:Placental Extracts; D007073:Immunoglobulin E; D010047:Ovalbumin