title: [Interaction of lipid membranes and neutral polymers by differential scanning calorimetry (DSC)].
abstract: Polymer-free and polymer-bearing liposomes from dimyristoylphosphatidylcholine (DMPC), dimyristoylphosphatidylglycerol (DMPG) and cholesterol (Ch) 10:1:1 (w/w/w) were prepared. Polymer-bearing liposomes were formed by incorporating an uncharged polymer [poly(vinyl alcohol) (PVA), poly(vinyl alcohol-co-vinylacetal) (PVA-Al), poly(vinyl alcohol-co-vinyl propional) (PVA-Prol) poly(vinyl alcohol-co-vinyl butiral) (PVA-Bul) copolymer, polyvinyl pyrrolidone (PVP) or polyethylene glycol (PEG) respectively]. The effect of some polymers on the phase transition parameter of phospholipids (DMPC and DMPG) has been studied by differential scanning calorimetry (DSC). DSC has become a standard technique for studying the thermally induced transition of phospholipid molecules from an ordered crystalline-like state at low temperature (gel phase) to a liquid crystalline-like state at higher temperature. The interaction between phospholipids and polymers was characterized by the alteration of the phase transition parameters (Tp, Tm, delta T1/2, delta Hp, delta Hm) measured by DSC. It was observed that some phase transition parameters were affected by all polymers but in different extent. PVA-Prol copolymer exhibited a prominent effect in increasing the membrane cooperativity. The influence of PVA-Bul was unique; it decreased the cooperativity of phospholipid molecules. It was also shown that the lipid membranepolymer interaction considerably depends on both the chemical structure and the molecular mass of the polymers.
authors.affiliation: Eötvös Loránd Tudományegyetem, Kolloidkémiai és Kolloidtechnológiai Tanszék, Budapest.
authors.forename: F L
authors.initials: FL
authors.lastname: Grohmann
authors.forename: M
authors.initials: M
authors.lastname: Szógyi
authors.forename: F
authors.initials: F
authors.lastname: Csempesz
mesh_terms: D002152:Calorimetry, Differential Scanning; D002784:Cholesterol; D004134:Dimyristoylphosphatidylcholine / Q000737:chemistry*; D008081:Liposomes / Q000737:chemistry*; D010715:Phosphatidylglycerols / Q000737:chemistry*; D011092:Polyethylene Glycols / Q000737:chemistry*; D011142:Polyvinyl Alcohol / Q000737:chemistry*; D011206:Povidone-Iodine / Q000737:chemistry*
chemical_list: D008081:Liposomes; D010715:Phosphatidylglycerols; D011092:Polyethylene Glycols; D011206:Povidone-Iodine; D011142:Polyvinyl Alcohol; D002784:Cholesterol; C002773:dimyristoylphosphatidylglycerol; D004134:Dimyristoylphosphatidylcholine
vernacular_title: Lipidmembránok és neutrális polimerek kölcsönhatásának differenciál "scanning" kalorimetriás (DSC) vizsgálata.