Evaluation of the 5' spliced form of human cathepsin B mRNA in colorectal mucosa and tumors.
To evaluate the 5' spliced form of human cathepsin B mRNA in colorectal mucosa and tumors, we have determined the ratio of the spliced form for the exon 2/the complete form of cathepsin B mRNAs obtained by RT-PCR. Such ratio is significantly higher in colorectal tumors than in colorectal mucosa (p < 0.05, Kruskal-Wallis test) or in skeletal muscle (p < 0.05). Moreover, 2-fold more complete form than the spliced mRNA was found in the tumors than in the colorectal mucosa. Our data indicate that the alternative splicing of human cathepsin B mRNA in the 5'UTR may be considered as an indicator of the cellular transformation, in colorectal cancer.
Hizel
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Laboratoire d'Oncologie Moléculaire, INSERM CRI 9402, Centre Jean Perrin, 58 rue Montalembert, B.P. 392, 63011 Clermont-Ferrand Cedex 1, France.
Ferrara
M
M
Cure
H
H
Pezet
D
D
Dechelotte
P
P
Chipponi
J
J
Rio
P
P
Bignon
Y J
YJ
Bernard-Gallon
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D
D017398:Alternative Splicing; D002401:Cathepsin B / Q000096:biosynthesis* / Q000235:genetics; D003106:Colon / Q000201:enzymology; D015179:Colorectal Neoplasms / Q000201:enzymology* / Q000473:pathology; D005091:Exons; D006801:Humans; D007413:Intestinal Mucosa / Q000201:enzymology* / Q000473:pathology; D007700:Kinetics; D018482:Muscle, Skeletal / Q000201:enzymology; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000096:biosynthesis*; D012007:Rectum / Q000201:enzymology; D014407:Tumor Cells, Cultured
D012333:RNA, Messenger; D002401:Cathepsin B