title: Resuspension-induced ion flux.
abstract: Pelleting and resuspension of Fura 2-labeled c6 glioma cells leads to a large Ca flux characterized by a high initial internal level of Ca which rapidly declines to close to basal levels. The effect has been termed resuspension-induced ion flux (RIIF). The RIIF effect is temperature dependent and requires external calcium, cytoskeletal integrity, and functional calcium and potassium channels. The magnitude of the RIIF effect is dependent upon pelleting speed, suggesting cell contact and reduction in external fluid medium to be important causative parameters. Several other cell species also exhibit the RIIF effect.
authors.affiliation: New Ideas, Co., Rockville, Maryland 20852, USA.
authors.forename: D B
authors.initials: DB
authors.lastname: Millar
mesh_terms: D000818:Animals; D002118:Calcium / Q000378:metabolism*; D015220:Calcium Channels / Q000378:metabolism; D018929:Cell Culture Techniques; D002460:Cell Line; D002498:Centrifugation; D002614:Chelating Agents / Q000494:pharmacology; D003078:Colchicine / Q000494:pharmacology; D004533:Egtazic Acid / Q000494:pharmacology; D005456:Fluorescent Dyes; D016257:Fura-2; D006801:Humans; D017136:Ion Transport / Q000187:drug effects; D015221:Potassium Channels / Q000378:metabolism; D051381:Rats; D013696:Temperature
chemical_list: D015220:Calcium Channels; D002614:Chelating Agents; D005456:Fluorescent Dyes; D015221:Potassium Channels; D004533:Egtazic Acid; D003078:Colchicine; D002118:Calcium; D016257:Fura-2