title: The DUG gene of Drosophila melanogaster encodes a structural and functional homolog of the S. cerevisiae SUG1 predicted ATPase associated with the 26S proteasome.
abstract: The DUG gene of Drosophila encodes a putative ATPase that is a structural and functional homolog of the yeast SUG1 product. When introduced into S. cerevisiae, the Drosophila DUG gene rescued the lethality associated with a SUG1 mutant. Anti-DUG antibodies recognized a protein that migrated in high molecular weight complexes, along with components of the 26S proteasome, and also immunoprecipitated components of the 26S proteasome from embryonic extracts. Proteins recognized by the affinity-purified antibody raised against DUG were localized in either a punctate cytoplasmic distribution or in the nucleus, depending on the cell type, consistent with the subcellular localization of the 26S proteasome in various cell types.
authors.affiliation: Department of Developmental Biology, Beckman Center, B300, Stanford University School of Medicine, Stanford, CA 94305, USA.
authors.forename: L C
authors.initials: LC
authors.lastname: Mounkes
authors.forename: M T
authors.initials: MT
authors.lastname: Fuller
mesh_terms: D000251:Adenosine Triphosphatases / Q000096:biosynthesis / Q000235:genetics* / Q000276:immunology; D000818:Animals; D003001:Cloning, Molecular; D017124:Conserved Sequence; D003429:Cross Reactions; D004331:Drosophila melanogaster / Q000201:enzymology / Q000235:genetics*; D005656:Fungal Proteins / Q000235:genetics*; D017353:Gene Deletion; D018507:Gene Expression Regulation, Developmental; D017344:Genes, Insect; D046911:Macromolecular Substances; D008969:Molecular Sequence Data; D010447:Peptide Hydrolases / Q000235:genetics* / Q000276:immunology; D011233:Precipitin Tests; D046988:Proteasome Endopeptidase Complex; D012097:Repressor Proteins / Q000235:genetics*; D012441:Saccharomyces cerevisiae / Q000201:enzymology / Q000235:genetics*; D029701:Saccharomyces cerevisiae Proteins; D017386:Sequence Homology, Amino Acid; D013347:Subcellular Fractions / Q000276:immunology / Q000378:metabolism
chemical_list: D005656:Fungal Proteins; D046911:Macromolecular Substances; D012097:Repressor Proteins; D029701:Saccharomyces cerevisiae Proteins; D010447:Peptide Hydrolases; D046988:Proteasome Endopeptidase Complex; C061553:ATP dependent 26S protease; D000251:Adenosine Triphosphatases; C069670:RPT6 protein, S cerevisiae