HL60 cells exhibit particular ultrastructural features during all-trans retinoic acid-induced apoptosis.
Ultrastructural features induced by 1 micromol/l all-trans retinoic acid (ATRA) treatment of HL60 cells were observed with transmission electron microscopy. Whilst some cells were unaffected, most of the others underwent granulocytic differentiation, starting point for apoptosis and then, possibly, secondary necrosis. First steps of apoptosis led to nuclear fragmentation into dense bodies. Then three different ways were observed: i) cells shrank and dense bodies were expelled, mostly associated with a fine lamellae of cytoplasm, ii) a secondary necrosis involved the release of apoptotic bodies without cytoplasm or iii) cells showed a dual or bipolar structure, not previously described.
Bobichon
H
H
IFR53 - EA2063, Laboratoire de Biochimie, Faculte de Pharmacie, 51 rue Cognacq-Jay, 51096 Reims Cedex, France.
Mayer
P
P
Carpentier
Y
Y
Desoize
B
B
D017209:Apoptosis / Q000187:drug effects; D002454:Cell Differentiation; D002455:Cell Division / Q000187:drug effects; D002467:Cell Nucleus / Q000187:drug effects / Q000648:ultrastructure; D002470:Cell Survival; D002843:Chromatin / Q000187:drug effects / Q000648:ultrastructure; D004305:Dose-Response Relationship, Drug; D018922:HL-60 Cells / Q000166:cytology / Q000187:drug effects / Q000648:ultrastructure*; D006801:Humans; D008854:Microscopy, Electron; D014212:Tretinoin / Q000494:pharmacology*
D002843:Chromatin; D014212:Tretinoin