DNA-synthesis inhibition and repair DNA-synthesis in CHO Ade-C cells: an alternative approach to genotoxicity testing.
We describe an alternative assay to determine genotoxicity. Its main feature is that it combines two measures in a single experiment; the inhibition of replicative DNA synthesis together with the stimulation of DNA repair. We show that, in tests of four different genotoxic agents, the assay gives results that are entirely consistent with what is known about the mode of action of these agents. In addition, we have demonstrated that chemical carcinogens requiring metabolic activation can be examined using a standard procedure of incubation with a microsomal activating fraction. We consider the combined assay for DNA synthesis inhibition and repair synthesis to be a useful way for the rapid prescreening of chemicals suspected of genotoxic activity on the level of mammalian cells.
Slamenová
D
D
Cancer Research Institute of Slovak Academy of Sciences, Bratislava, Slovakia.
Collins
A
A
Wsólová
L
L
Papsová
E
E
Gábelová
A
A
Dusinská
M
M
D000818:Animals; D001580:Benzopyrenes / Q000494:pharmacology*; D001711:Biotransformation; D016466:CHO Cells; D006224:Cricetinae; D004260:DNA Repair / Q000187:drug effects*; D004261:DNA Replication / Q000187:drug effects*; D005069:Evaluation Studies as Topic; D019271:Hypoxanthine / Q000378:metabolism; D008770:Methylnitrosourea / Q000494:pharmacology; D009152:Mutagenicity Tests; D009153:Mutagens / Q000494:pharmacology*; D009600:Nitroquinolines / Q000494:pharmacology
D001580:Benzopyrenes; D009153:Mutagens; D009600:Nitroquinolines; D019271:Hypoxanthine; D008770:Methylnitrosourea