title: Catalytic properties of class A beta-lactamases: efficiency and diversity.
abstract: beta-Lactamases are the main cause of bacterial resistance to penicillins, cephalosporins and related beta-lactam compounds. These enzymes inactivate the antibiotics by hydrolysing the amide bond of the beta-lactam ring. Class A beta-lactamases are the most widespread enzymes and are responsible for numerous failures in the treatment of infectious diseases. The introduction of new beta-lactam compounds, which are meant to be 'beta-lactamase-stable' or beta-lactamase inhibitors, is thus continuously challenged either by point mutations in the ubiquitous TEM and SHV plasmid-borne beta-lactamase genes or by the acquisition of new genes coding for beta-lactamases with different catalytic properties. On the basis of the X-ray crystallography structures of several class A beta-lactamases, including that of the clinically relevant TEM-1 enzyme, it has become possible to analyse how particular structural changes in the enzyme structures might modify their catalytic properties. However, despite the many available kinetic, structural and mutagenesis data, the factors explaining the diversity of the specificity profiles of class A beta-lactamases and their amazing catalytic efficiency have not been thoroughly elucidated. The detailed understanding of these phenomena constitutes the cornerstone for the design of future generations of antibiotics.
authors.affiliation: Centre for Protein Engineering and Laboratoire d'Enzymologie, Université de Liège, Institut de Chimie B6, 4000 Liège (Sart Tilman), Belgium.
authors.forename: A
authors.initials: A
authors.lastname: Matagne
authors.forename: J
authors.initials: J
authors.lastname: Lamotte-Brasseur
authors.forename: J M
authors.initials: JM
authors.lastname: Frère
mesh_terms: D002384:Catalysis; D018360:Crystallography, X-Ray; D008956:Models, Chemical; D008958:Models, Molecular; D011487:Protein Conformation; D018440:beta-Lactam Resistance; D001618:beta-Lactamases / Q000378:metabolism*
chemical_list: D001618:beta-Lactamases