Use of T4 DNA polymerase to create cohesive termini in PCR products for subcloning and site-directed mutagenesis.
Cooney
A J
AJ
Department of Cell Biology, Baylor College of Medicine, Houston, TX 77030, USA. acooney@bcm.tmc.edu
D003001:Cloning, Molecular; D004259:DNA-Directed DNA Polymerase; D016297:Mutagenesis, Site-Directed; D016133:Polymerase Chain Reaction / Q000379:methods*; D014764:Viral Proteins / Q000378:metabolism*
D014764:Viral Proteins; C069983:gene 43 protein, Enterobacteria phage T4; D004259:DNA-Directed DNA Polymerase