The neural cell adhesion molecule L1: genomic organisation and differential splicing is conserved between man and the pufferfish Fugu.
The human gene for the neural cell adhesion molecule L1 is located on Xq28 between the ALD and MeCP2 loci. Mutations in the L1 gene are associated with four related neurological disorders, X-linked hydrocephalus, spastic paraplegia (SPG1), MASA syndrome, and X-linked corpus callosum agenesis. The clinical relevance of L1 has led us to sequence the L1 gene in human and to investigate its conservation in the vertebrate model genome of the pufferfish, Fugu rubripes (Fugu), a species with a compact genome of around 40Mb. For this purpose we have sequenced a human and a Fugu cosmid clone containing the corresponding L1 genes. For comparison, we have also amplified and sequenced the complete Fugu L1 cDNA. We find that the genomic structure of L1 is conserved. The human and Fugu L1 gene both have 28 exons of nearly identical size. Differential splicing of exons 2 and 27 is conserved over 430 million years, the evolutionary time span between the teleost Fugu and the human L1 gene. In contrast to previously published Fugu genes, many introns are larger in the Fugu L1 gene, making it slightly larger in size despite the compact nature of the Fugu genome. Homology at the amino acid and the nucleotide level with 40% and 51%, respectively, is lower than that of any previously reported Fugu gene. At the level of protein structure, both human and Fugu L1 molecules are composed of six immunoglobulin (Ig)-like domains and five fibronectin (Fn) type III domains, followed by a transmembrane domain and a short cytoplasmic domain. Only the transmembrane and the cytoplasmic domains are significantly conserved in Fugu, supporting their proposed function in intracellular signalling and interaction with cytoskeletal elements in the process of neurite outgrowth and fascicle formation. Our results show that the cytoplasmic domain can be further subdivided into a conserved and a variable region, which may correspond to different functions. Most pathological missense mutations in human L1 affect conserved residues. Fifteen out of 22 reported missense mutations alter amino acids that are identical in both species.
Coutelle
O
O
Institute of Molecular Biotechnology, Department of Genome Analysis, Beutenbergstrasse 11, 07745, Jena, Germany.
Nyakatura
G
G
Taudien
S
S
Elgar
G
G
Brenner
S
S
Platzer
M
M
Drescher
B
B
Jouet
M
M
Kenwrick
S
S
Rosenthal
A
A
D017398:Alternative Splicing; D000595:Amino Acid Sequence; D000818:Animals; D002462:Cell Membrane / Q000737:chemistry; D017124:Conserved Sequence; D003593:Cytoplasm / Q000737:chemistry; D019143:Evolution, Molecular; D005091:Exons; D005400:Fishes, Poisonous / Q000235:genetics*; D006031:Glycosylation; D006801:Humans; D007438:Introns; D039841:Leukocyte L1 Antigen Complex; D008969:Molecular Sequence Data; D009154:Mutation; D009422:Nervous System Diseases / Q000235:genetics; D019006:Neural Cell Adhesion Molecules / Q000737:chemistry / Q000235:genetics* / Q000502:physiology; D009842:Oligopeptides; D016415:Sequence Alignment; D017385:Sequence Homology; D014960:X Chromosome
D039841:Leukocyte L1 Antigen Complex; D019006:Neural Cell Adhesion Molecules; D009842:Oligopeptides; C047981:arginyl-glycyl-aspartic acid