title: Functional complementation by electroporation of human BACs into mammalian fibroblast cells.
abstract: Bacterial Artificial Chromosomes (BACs) have been used to complement a metabolic defect and to transfer a drug resistance marker into mammalian cells by electroporation. The selectable markers are stable and the recipient cells have BAC DNA integrated into the chromosomes as shown by fluorescent in situ hybridization, PCR and Southern hybridization.
authors.affiliation: Department of Molecular and Medical Genetics, L103, Oregon Health Sciences University, 3181 SW Sam Jackson Park Road, Portland, OR 97201, USA.
authors.forename: J A
authors.initials: JA
authors.lastname: Hejna
authors.forename: P L
authors.initials: PL
authors.lastname: Johnstone
authors.forename: S L
authors.initials: SL
authors.lastname: Kohler
authors.forename: D A
authors.initials: DA
authors.lastname: Bruun
authors.forename: C A
authors.initials: CA
authors.lastname: Reifsteck
authors.forename: S B
authors.initials: SB
authors.lastname: Olson
authors.forename: R E
authors.initials: RE
authors.lastname: Moses
mesh_terms: D001483:Base Sequence; D002460:Cell Line; D002876:Chromosomes, Bacterial / Q000235:genetics*; D017931:DNA Primers / Q000235:genetics; D018274:Electroporation; D018014:Gene Transfer Techniques; D005816:Genetic Complementation Test; D005819:Genetic Markers; D005821:Genetic Techniques; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D016133:Polymerase Chain Reaction
chemical_list: D017931:DNA Primers; D005819:Genetic Markers