Selective stimulation of jugular ganglion afferent neurons in guinea pig airways by hypertonic saline.
We evaluated the ability of hyperosmolar stimuli to activate afferent nerves in the guinea pig trachea and main bronchi and investigated the neural pathways involved. By using electrophysiological techniques, studies in vitro examined the effect of hyperosmolar solutions of sodium chloride (hypertonic saline) on guinea pig airway afferent nerve endings arising from either vagal nodose or jugular ganglia. The data reveal a differential sensitivity of airway afferent neurons to activation with hypertonic saline. Afferent fibers (both A delta and C fibers) with cell bodies located in jugular ganglia were much more sensitive to stimulation with hypertonic saline, compared with afferent neurons with cell bodies located in nodose ganglia. Additional studies in vivo demonstrated that inhalation of aerosols of hypertonic saline induced plasma extravasation in guinea pig trachea that was mediated via tachykinin NK1 receptors. Identification of a differential sensitivity of guinea pig airway afferent nerves to hypertonic saline leads to the speculation that airway responses to hyperosmolar stimuli may result from activation of afferent neurons originating predominantly from the jugular ganglion.
Pedersen
K E
KE
Department of Medicine, Johns Hopkins University School of Medicine, Baltimore, Maryland 21224, USA.
Meeker
S N
SN
Riccio
M M
MM
Undem
B J
BJ
D000280:Administration, Inhalation; D000818:Animals; D001980:Bronchi / Q000098:blood supply / Q000187:drug effects* / Q000294:innervation / Q000502:physiology; D002199:Capillary Permeability / Q000187:drug effects; D006168:Guinea Pigs; D066298:In Vitro Techniques; D008297:Male; D009412:Nerve Fibers / Q000187:drug effects / Q000502:physiology; D009475:Neurons, Afferent / Q000187:drug effects* / Q000502:physiology; D009620:Nodose Ganglion / Q000187:drug effects / Q000502:physiology; D018040:Receptors, Neurokinin-1 / Q000502:physiology; D012462:Saline Solution, Hypertonic / Q000494:pharmacology*; D014132:Trachea / Q000098:blood supply / Q000187:drug effects* / Q000294:innervation / Q000502:physiology; D014630:Vagus Nerve / Q000166:cytology / Q000187:drug effects* / Q000502:physiology
D018040:Receptors, Neurokinin-1; D012462:Saline Solution, Hypertonic