Functional complementation by electroporation of human BACs into mammalian fibroblast cells.
Bacterial Artificial Chromosomes (BACs) have been used to complement a metabolic defect and to transfer a drug resistance marker into mammalian cells by electroporation. The selectable markers are stable and the recipient cells have BAC DNA integrated into the chromosomes as shown by fluorescent in situ hybridization, PCR and Southern hybridization.
Hejna
J A
JA
Department of Molecular and Medical Genetics, L103, Oregon Health Sciences University, 3181 SW Sam Jackson Park Road, Portland, OR 97201, USA.
Johnstone
P L
PL
Kohler
S L
SL
Bruun
D A
DA
Reifsteck
C A
CA
Olson
S B
SB
Moses
R E
RE
D001483:Base Sequence; D002460:Cell Line; D002876:Chromosomes, Bacterial / Q000235:genetics*; D017931:DNA Primers / Q000235:genetics; D018274:Electroporation; D018014:Gene Transfer Techniques; D005816:Genetic Complementation Test; D005819:Genetic Markers; D005821:Genetic Techniques; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D016133:Polymerase Chain Reaction
D017931:DNA Primers; D005819:Genetic Markers