title: Functional complementation of nontoxic mutant binary toxins of Bacillus sphaericus 1593M generated by site-directed mutagenesis.
abstract: Alanine residues were substituted by site-directed mutagenesis at selected sites of the N- and C-terminal regions of the binary toxin (51- and 42-kDa peptides) of B. sphaericus 1593M, and the mutant toxins were cloned and expressed in Escherichia coli. Bioassays with mosquito larvae, using binary toxins derived from individual mutants, showed that the substitution of alanine at some sites in both the 51-kDa and the 42-kDa peptides resulted in a total loss of activity. Surprisingly, after mixing two nontoxic derivatives of the same peptide, i.e., one mutated at the N-terminal end and the other mutated at the C-terminal end of either the 51-kDa or the 42-kDa peptide, the toxicity was restored. This result indicates that the altered binary toxins can functionally complement each other by forming oligomers.
authors.affiliation: Centre for Biotechnology, Anna University, Madras, India.
authors.forename: M
authors.initials: M
authors.lastname: Shanmugavelu
authors.forename: F
authors.initials: F
authors.lastname: Rajamohan
authors.forename: M
authors.initials: M
authors.lastname: Kathirvel
authors.forename: G
authors.initials: G
authors.lastname: Elangovan
authors.forename: D H
authors.initials: DH
authors.lastname: Dean
authors.forename: K
authors.initials: K
authors.lastname: Jayaraman
mesh_terms: D000818:Animals; D001407:Bacillus / Q000472:pathogenicity*; D001427:Bacterial Toxins / Q000737:chemistry / Q000494:pharmacology*; D009033:Culicidae; D008970:Molecular Weight; D009032:Mosquito Control; D016297:Mutagenesis, Site-Directed; D009154:Mutation; D013329:Structure-Activity Relationship
chemical_list: D001427:Bacterial Toxins