Optimal 96-well plate set-up to avoid ethanol volatility when assessing ethanol cytotoxicity.
An optimal design has been established that maintains a constant level of ethanol when utilizing specific wells within a 96-well culture plate. A control (non-ethanol-containing) column is also included within each plate for direct comparisons of the cytotoxic effects of a given concentration of ethanol.
Mitchell
J J
JJ
Department of Neuroscience, University of Florida College of Medicine, Gainesville 32610-0244, USA. mitchell@ufbi.ufl.edu
Paiva
M
M
Heaton
M B
MB
D000818:Animals; D002470:Cell Survival / Q000187:drug effects; D046508:Culture Techniques; D000431:Ethanol / Q000494:pharmacology / Q000633:toxicity*; D006624:Hippocampus / Q000187:drug effects / Q000201:enzymology; D051381:Rats; D018675:Toxicity Tests / Q000379:methods*; D014835:Volatilization
D000431:Ethanol