title: The structure of GABPalpha/beta: an ETS domain- ankyrin repeat heterodimer bound to DNA.
abstract: GA-binding protein (GABP) is a transcriptional regulator composed of two structurally dissimilar subunits. The alpha subunit contains a DNA-binding domain that is a member of the ETS family, whereas the beta subunit contains a series of ankyrin repeats. The crystal structure of a ternary complex containing a GABPalpha/beta ETS domain-ankyrin repeat heterodimer bound to DNA was determined at 2. 15 angstrom resolution. The structure shows how an ETS domain protein can recruit a partner protein using both the ETS domain and a carboxyl-terminal extension and provides a view of an extensive protein-protein interface formed by a set of ankyrin repeats. The structure also reveals how the GABPalpha ETS domain binds to its core GGA DNA-recognition motif.
authors.affiliation: Department of Biophysics and Biophysical Chemistry and the Howard Hughes Medical Institute, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA.
authors.forename: A H
authors.initials: AH
authors.lastname: Batchelor
authors.forename: D E
authors.initials: DE
authors.lastname: Piper
authors.forename: F C
authors.initials: FC
authors.lastname: de la Brousse
authors.forename: S L
authors.initials: SL
authors.lastname: McKnight
authors.forename: C
authors.initials: C
authors.lastname: Wolberger
mesh_terms: D000595:Amino Acid Sequence; D017487:Ankyrins / Q000737:chemistry; D018360:Crystallography, X-Ray; D004247:DNA / Q000378:metabolism*; D004268:DNA-Binding Proteins / Q000737:chemistry* / Q000378:metabolism*; D019281:Dimerization; D051268:GA-Binding Protein Transcription Factor; D006860:Hydrogen Bonding; D008958:Models, Molecular; D008969:Molecular Sequence Data; D011487:Protein Conformation; D017433:Protein Structure, Secondary; D011518:Proto-Oncogene Proteins / Q000737:chemistry / Q000378:metabolism; D050783:Proto-Oncogene Proteins c-ets; D011994:Recombinant Proteins / Q000737:chemistry / Q000378:metabolism; D015534:Trans-Activators / Q000737:chemistry / Q000378:metabolism; D014157:Transcription Factors / Q000737:chemistry* / Q000378:metabolism*; D000099232:Proto-Oncogene Protein Spi-1
chemical_list: D017487:Ankyrins; D004247:DNA; D004268:DNA-Binding Proteins; D051268:GA-Binding Protein Transcription Factor; D011518:Proto-Oncogene Proteins; D050783:Proto-Oncogene Proteins c-ets; D011994:Recombinant Proteins; D015534:Trans-Activators; D014157:Transcription Factors; D000099232:Proto-Oncogene Protein Spi-1