title: The binding potential of commercial antibody conjugates with sera of various small terrestrial mammals.
abstract: Infectious diseases of wild animals are of increasing importance, both from an economic viewpoint and because several of these diseases are pathogenic to man. However, serosurveys to determine the circulation of infectious organisms in wildlife are complicated by the fact that antibodies to species-specific immunoglobulins are not available for use in serological assays such as enzyme-linked immunosorbent assays (ELISAs) or immunofluorescence assays. To determine the binding potential of four commercially available antibody conjugates with the sera of wild animals, sera from 27 species of small terrestrial mammals were allowed to react with alkaline phosphatase-labelled protein A, anti-rabbit IgG, anti-mouse IgG and anti-human IgG by by the use of an ELISA. It was found that sera from some species of the order Lagomorpha bound optimally to anti-rabbit IgG, while anti-mouse IgG could be used for most species of Rodentia. For all Carnivora, Insectivora, Macroscelidea, Hyracoidea and other Rodentia, staphylococcal protein A demonstrated optimal binding. None of the sera that was tested bound to anti-human IgG. These results demonstrate that commercial conjugates can be used in serological assays in which wild animal sera are used, and should be useful for future serosurveys to determine the circulation of infectious agents in small terrestrial mammals.
authors.affiliation: Department of Medical Microbiology, Faculty of Medicine, University of the Free State, Bloemfontein, South Africa.
authors.forename: A
authors.initials: A
authors.lastname: Pretorius
authors.forename: M J
authors.initials: MJ
authors.lastname: Oelofsen
authors.forename: M S
authors.initials: MS
authors.lastname: Smith
authors.forename: E
authors.initials: E
authors.lastname: van der Ryst
mesh_terms: D000818:Animals; D000835:Animals, Wild / Q000097:blood*; D000914:Antibodies, Viral; D002335:Carnivora / Q000097:blood; D004797:Enzyme-Linked Immunosorbent Assay; D007312:Eulipotyphla / Q000097:blood; D006801:Humans; D007043:Hyraxes / Q000097:blood; D007074:Immunoglobulin G / Q000097:blood*; D007793:Lagomorpha / Q000097:blood; D051379:Mice / Q000276:immunology; D011485:Protein Binding; D011817:Rabbits / Q000276:immunology; D012377:Rodentia / Q000097:blood; D013045:Species Specificity; D013205:Staphylococcal Protein A / Q000097:blood*
chemical_list: D000914:Antibodies, Viral; D007074:Immunoglobulin G; D013205:Staphylococcal Protein A