title: Enantiomeric separation of d-/l-norepinephrine and -epinephrine by high-performance liquid chromatography with a beta-cyclodextrin type chiral stationary phase.
abstract: Enantiomeric separation of d-/l-norepinephrine (NE) and d-/l-epinephrine (E) was investigated with various mobile phases by high-performance liquid chromatography using a beta-cyclodextrin type chiral stationary phase as a chiral column. The mobile phase of 2.5 M phosphate buffer (pH 3.0) gave separation factors (alpha s) of 1.04 and 1.06 for NE and E, respectively.
authors.affiliation: Faculty of Pharmaceutical Sciences, University of Tokyo, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Fukushima
authors.forename: K
authors.initials: K
authors.lastname: Murayama
authors.forename: T
authors.initials: T
authors.lastname: Santa
authors.forename: H
authors.initials: H
authors.lastname: Homma
authors.forename: K
authors.initials: K
authors.lastname: Imai
mesh_terms: D002021:Buffers; D002846:Chromatography, Affinity; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D003505:Cyclodextrins; D004837:Epinephrine / Q000737:chemistry / Q000302:isolation & purification*; D006863:Hydrogen-Ion Concentration; D009638:Norepinephrine / Q000737:chemistry / Q000302:isolation & purification*; D013237:Stereoisomerism; D047392:beta-Cyclodextrins
chemical_list: D002021:Buffers; D003505:Cyclodextrins; D047392:beta-Cyclodextrins; C031215:betadex; D009638:Norepinephrine; D004837:Epinephrine