Augmentation of sinecomitant immunity in mice by gamma-(9H-purine-6-yl)thiomethyl L-glutamate (6-MPG), a water-soluble derivative of 6-mercaptopurine.
Effects of the administration of gamma-(9H-purine-6-yl)thiomethyl L-glutamate (6-MPG), a water-soluble derivative of 6-mercaptopurine, on concomitant and sinecomitant immunity against the implanted MethA tumor were studied in BALB/c mice. In the concomitant immunity experiments, mice were intradermally inoculated with 1x10(5) MethA cells at the right inguinal region on day 0. In sinecomitant immunity experiments, mice were similarly inoculated on day -21, and the grown tumor was excised on day -11. Both the tumor-bearing and tumor-ectomized animals were re-inoculated with 3x10(6) MethA cells intradermally at the left inguinal region on day 10. Administration of 6-MPG (100 mg/kg, i.p.) on days 3 through 7 significantly inhibited growth of the re-inoculated tumor in both series of experiments. Cyclophosphamide, adriamycin, mitomycin C and cis-diamminedichloroplatinum (II) had no significant effect on the growth of the re-inoculated tumor in the tumor-ectomized mice. Spleen cells harvested from the 6-MPG-treated tumor-ectomized mice showed a strong tumor-neutralizing activity (Winn assay).
Kashida
T
T
Research Laboratories, Tanabe Seiyaku Co., Ltd., Toda, Saitama, Japan.
Narasaki
N
N
Tsujihara
K
K
Naito
K
K
Takeyama
S
S
D000818:Animals; D000970:Antineoplastic Agents / Q000494:pharmacology*; D017690:Cell Transplantation; D007111:Immunity, Cellular / Q000187:drug effects; D008297:Male; D015122:Mercaptopurine / Q000031:analogs & derivatives* / Q000494:pharmacology; D008748:Methylcholanthrene; D051379:Mice; D008807:Mice, Inbred BALB C; D009362:Neoplasm Metastasis / Q000276:immunology / Q000517:prevention & control*; D009368:Neoplasm Transplantation / Q000276:immunology; D009374:Neoplasms, Experimental / Q000139:chemically induced / Q000276:immunology* / Q000473:pathology; D009500:Neutralization Tests; D010641:Phenotype; D013154:Spleen / Q000187:drug effects / Q000276:immunology
C108537:(9H-purin-6-yl)thiomethyl glutamate; D000970:Antineoplastic Agents; D008748:Methylcholanthrene; D015122:Mercaptopurine