Spectrum of cytokine gene expression in intestinal mucosal lesions of Crohn's disease and ulcerative colitis.
OBJECTIVE
We investigated the mRNA expression of spectrum of cytokines in the colonic mucosa in inflammatory bowel disease (IBD).


METHODS
The expression of cytokine gene was evaluated by using the reverse transcription-polymerase chain reaction and the radioactivity of amplified cDNA standardized by coamplified beta-actin cDNA.


RESULTS
Crohn's disease and ulcerative colitis showed significantly increased expression of IL-1beta, IL-6, IL-8 and TNF-alpha mRNA as compared with controls (p < 0.05).


CONCLUSIONS
Proinflammatory cytokines such as IL-1beta, IL-6, IL-8 and TNF-alpha are closely involved in the immune abnormalities of inflammatory mucosal lesions in IBD.
Funakoshi
K
K
Third Department of Internal Medicine, Niigata University School of Medicine, Japan.
Sugimura
K
K
Anezaki
K
K
Bannai
H
H
Ishizuka
K
K
Asakura
H
H
D000293:Adolescent; D000328:Adult; D003093:Colitis, Ulcerative / Q000235:genetics / Q000276:immunology*; D003424:Crohn Disease / Q000235:genetics / Q000276:immunology*; D016207:Cytokines / Q000096:biosynthesis* / Q000235:genetics / Q000276:immunology; D005260:Female; D005770:Gastrointestinal Neoplasms / Q000235:genetics / Q000276:immunology*; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D008297:Male; D008875:Middle Aged; D016133:Polymerase Chain Reaction / Q000379:methods; D012333:RNA, Messenger / Q000378:metabolism; D012334:RNA, Neoplasm / Q000378:metabolism; D014158:Transcription, Genetic; D015854:Up-Regulation
D016207:Cytokines; D012333:RNA, Messenger; D012334:RNA, Neoplasm