Rice dwarf phytoreovirus segment S11 encodes a nucleic acid binding protein.
The function of rice dwarf virus segment 11 and the corresponding segments of other phytoreoviruses is not yet determined. The amino acid sequence of Pns11, encoded by segment 11, contains a putative zinc finger and five flanking basic regions at the C-terminus. The full-length Pns11 protein and three truncated derivatives, which lack the N-terminus, the zinc-finger or the C-terminal five basic regions were expressed in Escherichia coli and their nucleic acid binding properties were studied. Pns11 interacts with single- and double-stranded forms of DNA and RNA in a sequence-nonspecific manner. The truncated derivative which contains both the zinc-finger and the C-terminal basic regions has the same binding properties as the full-length Pns11. However, removal of either of these domains prevents binding activity. The binding activity of Pns11 was drastically reduced when the blots were treated with a high concentration of EDTA. Moreover, Pns11 extracted from infected rice also binds to single-stranded RNA. These data suggest that RDV Pns11 binding activity is structure-dependent and it may play an important role in virus replication and/or genome assortment.
Xu
H
H
National Laboratory of Protein Engineering and Plant Genetic Engineering, College of Life Sciences, Peking University, Beijing, China.
Li
Y
Y
Mao
Z
Z
Li
Y
Y
Wu
Z
Z
Qu
L
L
An
C
C
Ming
X
X
Schiemann
J
J
Casper
R
R
Chen
Z
Z
D000595:Amino Acid Sequence; D004268:DNA-Binding Proteins / Q000235:genetics*; D004492:Edetic Acid / Q000494:pharmacology; D004926:Escherichia coli / Q000235:genetics; D015870:Gene Expression; D018014:Gene Transfer Techniques; D005814:Genes, Viral / Q000235:genetics*; D008969:Molecular Sequence Data; D010940:Plant Proteins / Q000235:genetics*; D012087:Reoviridae / Q000235:genetics*; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid; D014764:Viral Proteins
D004268:DNA-Binding Proteins; D010940:Plant Proteins; D014764:Viral Proteins; C070922:dwarf phytoreovirus segment 11 protein, rice dwarf phytoreovirus; D004492:Edetic Acid