Induction of apoptotic cell death in HL-60 cells by acteoside, a phenylpropanoid glycoside.
Acteoside, a phenylpropanoid glycoside with anti-oxidative activity, induced cell death in promyelocytic leukemia HL-60 cells with an IC50 value of 26.7/microM. Analysis of extracted DNA on agarose gel electrophoresis revealed that acteoside induced the internucleosomal breakdown of chromatin DNA characteristic of apoptosis. Apoptosis-specific DNA fragmentation was clearly detectable 4 h after treatment with acteoside and was independent of the cell cycle phase. These data indicate that acteoside induces apoptosis in HL-60 cells.
Inoue
M
M
Department of Pharmacognosy, Faculty of Pharmaceutical Sciences, Nagoya City University, Japan.
Sakuma
Z
Z
Ogihara
Y
Y
Saracoglu
I
I
D000975:Antioxidants / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects*; D053938:DNA Fragmentation; D004587:Electrophoresis, Agar Gel; D005434:Flow Cytometry; D005960:Glucosides / Q000494:pharmacology*; D018922:HL-60 Cells; D006801:Humans; D007202:Indicators and Reagents; D010636:Phenols; D059808:Polyphenols
D000975:Antioxidants; D005960:Glucosides; D007202:Indicators and Reagents; D010636:Phenols; C058956:acteoside; D059808:Polyphenols