Intracellular production of African horsesickness virus core-like particles by expression of the two major core proteins, VP3 and VP7, in insect cells.
To gain more insight into the structure of the African horsesickness virus (AHSV) core particle, we have cloned, partially characterized and expressed the two major core proteins, VP3 and VP7, of AHSV-9. VP7 was found to be highly conserved amongst different serotypes. The VP3 and VP7 genes were subsequently expressed in insect cells by means of recombinant baculoviruses. VP7 was synthesized to very high levels and aggregated into distinctive crystals. Co-expression of VP3 and VP7 resulted in the intracellular formation of core-like particles which structurally resembled empty AHSV cores.
Maree
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Biochemistry Division, Onderstepoort Veterinary Institute, South Africa.
Durbach
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Huismans
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D000355:African Horse Sickness / Q000821:virology*; D000356:African Horse Sickness Virus / Q000145:classification / Q000235:genetics* / Q000648:ultrastructure; D000818:Animals; D000956:Antigens, Viral / Q000096:biosynthesis* / Q000737:chemistry; D016367:Baculoviridae; D001483:Base Sequence; D002213:Capsid / Q000096:biosynthesis* / Q000737:chemistry; D036022:Capsid Proteins; D002460:Cell Line; D017931:DNA Primers; D006736:Horses; D007313:Insecta; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000737:chemistry; D012703:Serotyping; D014162:Transfection; D014758:Viral Core Proteins / Q000096:biosynthesis* / Q000737:chemistry
D000956:Antigens, Viral; D036022:Capsid Proteins; D017931:DNA Primers; D011994:Recombinant Proteins; D014758:Viral Core Proteins; C069264:VP7 major core antigen, African horsesickness virus