title: Generation of growth hormone binding protein by avian growth plate chondrocytes is dependent on cell differentiation.
abstract: Growth hormone receptor (GH-R) gene expression was evaluated in avian growth-plates in situ and in cultured chondrocytes. In the epiphyseal growth-plate, chondrocytes at different stages of differentiation located at the proliferative and upper hypertrophic zones express the GH-R gene. In culture, addition of ascorbic acid facilitated chondrocyte differentiation as evaluated by decrease in collagen type II gene expression and increase in alkaline phosphatase activity and osteopontin gene expression. Both the ascorbic acid-treated and untreated chondrocytes expressed the gene coding for the chicken growth hormone receptor (cGH-R), but only the undifferentiated cells were capable of binding the hormone. This reduction in GH-binding resulted in alteration in GH-dependent regulation of the GH-R gene expression: only the undifferentiated chondrocytes responded to chicken GH (cGH) by down-regulation of the cGH-R gene expression. Chondrocyte differentiation induced by either ascorbic acid or retinoic acid was associated with the appearance of two growth hormone binding-proteins (GHBPs) in the culture medium with estimated MWs of 32 and 70 kDa, respectively. These GHBPs differ in their MW from the major GHBP found in chicken plasma. Chondrocyte GHBPs specifically bind [125I]cGH, which can be displaced by an excess of unlabeled cGH. The differentiation-dependent increase in the 70 kDa GHBP was observed also using specific chicken GHBP antiserum. Our data suggest that the reduction of the differentiated chondrocytes response to GH is due to differentiation-dependent loss of the extracellular domain of the GH-R, resulting in a lack of functional receptors on the cell surface and generation of GHBP.
authors.affiliation: Institute of Animal Science, Agricultural Research Organization, The Volcani Center, Bet Dagan, Israel.
authors.forename: E
authors.initials: E
authors.lastname: Monsonego
authors.forename: W R
authors.initials: WR
authors.lastname: Baumbach
authors.forename: I
authors.initials: I
authors.lastname: Lavelin
authors.forename: A
authors.initials: A
authors.lastname: Gertler
authors.forename: S
authors.initials: S
authors.lastname: Hurwitz
authors.forename: M
authors.initials: M
authors.lastname: Pines
mesh_terms: D000818:Animals; D001205:Ascorbic Acid / Q000494:pharmacology; D001667:Binding, Competitive; D002454:Cell Differentiation; D002478:Cells, Cultured; D002645:Chickens; D003094:Collagen / Q000235:genetics; D017077:Culture Media, Conditioned; D015870:Gene Expression; D013006:Growth Hormone / Q000378:metabolism; D006132:Growth Plate / Q000378:metabolism*; D008970:Molecular Weight; D011986:Receptors, Somatotropin / Q000096:biosynthesis* / Q000235:genetics / Q000378:metabolism
chemical_list: D017077:Culture Media, Conditioned; D011986:Receptors, Somatotropin; D013006:Growth Hormone; D003094:Collagen; D001205:Ascorbic Acid