[Quality control of blood components by means of flow cytometers].
Small numbers of leukocytes cannot be counted by automated methods. So we performed fluorescent staining of leukocyte DNA and analyzed 50 samples of single-donor PC by FACS. In 72% of these PC we found white blood cells (WBC) < 5/microliter = WBC < 10(6)/U. Nevertheless we do filter our PC. Should fresh frozen plasma (FFP) be irradiated for immunocompromised patients to prevent TA-GvHD? To evaluate the number and distribution of WBC in FFP we stained WBC with moAb and performed FACS analyses. We found WBC 7.164 +/- 5.66 x 10(6)/U, lymphocytes, 3.032 +/- 3.66 x 10(6)/U and T lymphocytes 2.135 +/- 2.02 x 10(6)/U. According to these data it should be considered to irradiate FFP for immunocompromised patients.
Gilli
R
R
Department für Transfusionsmedizin und Immunhämatologie, Chirurgische Universitätsklinik Graz.
Truschnig-Wilders
M
M
Beaufort
F
F
Lanzer
G
G
D001782:Blood Donors; D001792:Blood Platelets; D005434:Flow Cytometry / Q000379:methods; D006801:Humans; D007937:Leukapheresis / Q000592:standards*; D007962:Leukocytes; D008214:Lymphocytes; D010983:Plateletpheresis / Q000592:standards*; D011786:Quality Control; D013601:T-Lymphocytes
Qualitätskontrolle von Blutkomponenten mittels Durchflusszytometer.