title: The Win1 mitotic regulator is a component of the fission yeast stress-activated Sty1 MAPK pathway.
abstract: The fission yeast Sty1 mitogen-activated protein (MAP) kinase (MAPK) and its activator the Wis1 MAP kinase kinase (MAPKK) are required for cell cycle control, initiation of sexual differentiation, and protection against cellular stress. Like the mammalian JNK/SAPK and p38/CSBP1 MAPKs, Sty1 is activated by a range of environmental insults including osmotic stress, hydrogen peroxide, UV light, menadione, heat shock, and the protein synthesis inhibitor anisomycin. We have recently identified two upstream regulators of the Wis1 MAPKK, namely the Wak1 MAPKKK and the Mcs4 response regulator. Cells lacking Mcs4 or Wak1, however, are able to proliferate under stressful conditions and undergo sexual differentiation, suggesting that additional pathway(s) control the Wis1 MAPKK. We now show that this additional signal information is provided, at least in part, by the Win1 mitotic regulator. We show that Wak1 and Win1 coordinately control activation of Sty1 in response to multiple environmental stresses, but that Wak1 and Win1 perform distinct roles in the control of Sty1 under poor nutritional conditions. Our results suggest that the stress-activated Sty1 MAPK integrates information from multiple signaling pathways.
authors.affiliation: Division of Yeast Genetics, National Institute for Medical Research, The Ridgeway, Mill Hill, London NW7 1AA, United Kingdom.
authors.forename: J C
authors.initials: JC
authors.lastname: Shieh
authors.forename: M G
authors.initials: MG
authors.lastname: Wilkinson
authors.forename: J B
authors.initials: JB
authors.lastname: Millar
mesh_terms: D000841:Anisomycin / Q000494:pharmacology; D002374:Catalase / Q000378:metabolism; D018797:Cell Cycle Proteins / Q000235:genetics / Q000502:physiology; D004789:Enzyme Activation; D015966:Gene Expression Regulation, Fungal / Q000187:drug effects / Q000502:physiology; D005800:Genes, Fungal / Q000502:physiology; D006358:Hot Temperature; D006861:Hydrogen Peroxide / Q000494:pharmacology; D020930:MAP Kinase Kinase Kinases; D020929:Mitogen-Activated Protein Kinase Kinases; D020928:Mitogen-Activated Protein Kinases; D008938:Mitosis; D009154:Mutation; D011189:Potassium Chloride / Q000494:pharmacology; D011494:Protein Kinases / Q000235:genetics / Q000378:metabolism*; D017346:Protein Serine-Threonine Kinases / Q000235:genetics / Q000502:physiology*; D017027:Protein Tyrosine Phosphatases / Q000235:genetics; D012331:RNA, Fungal / Q000032:analysis; D012333:RNA, Messenger / Q000032:analysis; D012568:Schizosaccharomyces / Q000201:enzymology* / Q000235:genetics / Q000254:growth & development; D029702:Schizosaccharomyces pombe Proteins; D015398:Signal Transduction / Q000502:physiology*; D013831:Thiamine / Q000502:physiology
chemical_list: D018797:Cell Cycle Proteins; C105788:Mcs4 protein, S pombe; D012331:RNA, Fungal; D012333:RNA, Messenger; D029702:Schizosaccharomyces pombe Proteins; D011189:Potassium Chloride; D000841:Anisomycin; D006861:Hydrogen Peroxide; D002374:Catalase; D011494:Protein Kinases; D017346:Protein Serine-Threonine Kinases; D020928:Mitogen-Activated Protein Kinases; C095409:sty1 protein, S pombe; D020930:MAP Kinase Kinase Kinases; C109170:win1 protein, S pombe; D020929:Mitogen-Activated Protein Kinase Kinases; C072068:wis1 protein, S pombe; D017027:Protein Tyrosine Phosphatases; C078759:pyp2 protein, S pombe; D013831:Thiamine