title: Computer analysis of the entire budding yeast genome for putative targets of the GCN4 transcription factor.
abstract: The completion of the yeast genome project enables an analysis of various phenomena for a whole eukaryotic genome. We aimed at characterizing a full spectrum of target genes for a transcription activator, and specifically characterized putative targets for GCN4 in the budding yeast. The results suggest that about 1% of the genes are regulated by GCN4 and that these genes code for proteins involved in amino-acid and nucleotide metabolism. Our analysis proposes that, when enough data about the binding nature of a transcription factor exists, it is possible to identify its putative targets and also to try and assign a physiological role for this transcription factor.
authors.affiliation: Department of Genetics, Institute of Life Sciences, The Hebrew University of Jerusalem, Givat-Ram, Jerusalem 91904, Israel.
authors.forename: O
authors.initials: O
authors.lastname: Schuldiner
authors.forename: C
authors.initials: C
authors.lastname: Yanover
authors.forename: N
authors.initials: N
authors.lastname: Benvenisty
mesh_terms: D001665:Binding Sites / Q000235:genetics; D002874:Chromosome Mapping; D019295:Computational Biology / Q000379:methods; D004268:DNA-Binding Proteins; D016208:Databases, Factual; D005656:Fungal Proteins / Q000235:genetics* / Q000378:metabolism; D005800:Genes, Fungal; D016681:Genome, Fungal; D005810:Multigene Family; D011494:Protein Kinases / Q000235:genetics* / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000235:genetics*; D029701:Saccharomyces cerevisiae Proteins; D012984:Software; D014157:Transcription Factors / Q000235:genetics* / Q000378:metabolism
chemical_list: D004268:DNA-Binding Proteins; D005656:Fungal Proteins; D029701:Saccharomyces cerevisiae Proteins; D014157:Transcription Factors; D011494:Protein Kinases