Extremely low frequency magnetic fields promote neurite varicosity formation and cell excitability in cultured rat chromaffin cells.
We report the use of an extremely low frequency magnetic field (ELF-MF, 60 Hz, 10 Grms) to stimulate cultured chromaffin cells to determine the possible changes that may occur in their electrical properties. The results showed that ELF-MF not only facilitated neurite outgrowth, but also formation of neurite varicosity with high concentration of catecholamines. This report also shows for the first time that a greater number of cultured chromaffin cells differentiated by ELF-MF have spontaneous extracellular electrical activity and that their firing frequency is higher than that seen in non-stimulated cells.
Zhang
L
L
Depto. de Fisiología, Universidad Nacional Autónoma de México, México, D.F.
Zhou
L
L
Vega-González
A
A
Mendoza
D
D
Drucker-Colín
R
R
D000200:Action Potentials; D000313:Adrenal Medulla / Q000166:cytology; D000818:Animals; D002395:Catecholamines / Q000032:analysis; D002454:Cell Differentiation; D002455:Cell Division; D002478:Cells, Cultured; D019439:Chromaffin Cells / Q000473:pathology*; D004574:Electromagnetic Fields; D007150:Immunohistochemistry; D009414:Nerve Growth Factors; D016501:Neurites / Q000737:chemistry / Q000473:pathology*; D018408:Patch-Clamp Techniques; D051381:Rats; D017208:Rats, Wistar; D014446:Tyrosine 3-Monooxygenase / Q000032:analysis
D002395:Catecholamines; D009414:Nerve Growth Factors; D014446:Tyrosine 3-Monooxygenase