title: The role of cadherin-catenin-cytoskeleton complex in angiogenesis: antisense oligonucleotide of plakoglobin promotes angiogenesis in vitro, and protein kinase C (PKC) enhances angiogenesis through the plakoglobin signaling pathway.
abstract: Angiogenesis plays an important role in various diseases and conditions such as malignant tumor, wound healing, and atherosclerosis. Since cell-to-cell adhesion may play a key role in angiogenesis, we investigated the effect of the cadherin-catenin-cytoskeleton complex on angiogenesis in human umbilical vein endothelial cells (HUVECs). Immunofluorescence staining revealed that alpha-catenin, beta-catenin, and plakoglobin were concentrated at cell-cell contacts in HUVECs. Antisense oligonucleotide (AS-oligo), complementary to the region of human plakoglobin was dissolved in saline and applied to the media at 1 mM every 12 h for 4 days, and sense oligonucleotide (S-oligo) was used as control. HUVEC migration from an injury line was enhanced by AS-oligo. Interestingly, HUVECs migrated in line with S-oligo, and in a scattered fashion with AS-oligo. Tube formation on Matrigel occurred earlier with AS-oligo than with S-oligo. These findings indicate that plakoglobin inhibited HUVEC migration and tube formation (angiogenesis) by regulating cell-cell adhesion.
authors.affiliation: Department of Cardiology, Heart Institute of Japan, Tokyo Women's Medical College, Japan.
authors.forename: H
authors.initials: H
authors.lastname: Nagashima
authors.forename: M
authors.initials: M
authors.lastname: Okada
authors.forename: C
authors.initials: C
authors.lastname: Hidai
authors.forename: S
authors.initials: S
authors.lastname: Hosoda
authors.forename: H
authors.initials: H
authors.lastname: Kasanuki
authors.forename: M
authors.initials: M
authors.lastname: Kawana
mesh_terms: D015820:Cadherins / Q000502:physiology*; D015815:Cell Adhesion Molecules / Q000502:physiology*; D002478:Cells, Cultured; D003598:Cytoskeletal Proteins / Q000502:physiology*; D051180:Desmoplakins; D004730:Endothelium, Vascular / Q000166:cytology / Q000502:physiology*; D005455:Fluorescent Antibody Technique; D006801:Humans; D018919:Neovascularization, Physiologic / Q000187:drug effects / Q000502:physiology*; D016376:Oligonucleotides, Antisense / Q000494:pharmacology; D016133:Polymerase Chain Reaction; D011493:Protein Kinase C / Q000502:physiology*; D015398:Signal Transduction / Q000502:physiology*; D015534:Trans-Activators; D014471:Umbilical Veins / Q000166:cytology; D051176:beta Catenin; D051185:gamma Catenin
chemical_list: C495270:CTNNB1 protein, human; D015820:Cadherins; D015815:Cell Adhesion Molecules; D003598:Cytoskeletal Proteins; D051180:Desmoplakins; D016376:Oligonucleotides, Antisense; D015534:Trans-Activators; D051176:beta Catenin; D051185:gamma Catenin; D011493:Protein Kinase C