title: A high frequency of Mamu-A*01 in the rhesus macaque detected by polymerase chain reaction with sequence-specific primers and direct sequencing.
abstract: SIV infection of rhesus macaques is an excellent model for HIV infection of humans. Unfortunately, it is has been difficult to identify macaques expressing particular MHC class I alleles. Here we describe the use of PCR-SSP for Mamu-A*01 typing of rhesus macaques. The Mamu-A*01 allele was amplified from genomic DNA using Mamu-A*01-specific primers and positive PCR products were directly sequenced. Our technique identified 15 Mamu-A*01-positive animals of 68 tested. We validated our molecular analysis by showing that lymphocytes from 8 Mamu-A*01-positive animals expressed Mamu-A*01 as determined by immunoprecipitation and 1-D IEF. The technical simplicity and accuracy of this typing method should facilitate selection of Mamu-A*01-positive rhesus macaques for AIDS virus pathogenesis and vaccine studies.
authors.affiliation: Wisconsin Regional Primate Research Center, University of Wisconsin, Madison 53715, USA.
authors.forename: L A
authors.initials: LA
authors.lastname: Knapp
authors.forename: E
authors.initials: E
authors.lastname: Lehmann
authors.forename: M S
authors.initials: MS
authors.lastname: Piekarczyk
authors.forename: J A
authors.initials: JA
authors.lastname: Urvater
authors.forename: D I
authors.initials: DI
authors.lastname: Watkins
mesh_terms: D000483:Alleles; D000818:Animals; D017931:DNA Primers; D005787:Gene Frequency; D005805:Genes, MHC Class I; D015395:Histocompatibility Antigens Class I / Q000235:genetics*; D008253:Macaca mulatta / Q000235:genetics* / Q000276:immunology; D016133:Polymerase Chain Reaction; D017422:Sequence Analysis, DNA
chemical_list: D017931:DNA Primers; D015395:Histocompatibility Antigens Class I; C110764:Mamu-A 01 antigen