Young inflorescence-bearing shoots with roots of Tradescantia clone BNL 4430 cultivated in nutrient solution circulating systems: an alternative to potted plants and cuttings for mutagenicity tests.
The use of young inflorescence-bearing shoots with roots of Tradescantia clone BNL 4430 cultivated in a nutrient solution circulating (NSC) growth chamber was tested and developed as an alternative method for using Tradescantia plants in mutagenicity testings. The NSC growth chamber was designed for our requirements, based on trial cultivations of the shoots with roots in its smaller-sized prototype. The nutrient solution used was a 1/2500 Hyponex solution. The characteristics of this clone, i.e., many new shoots constantly emerging from the basal nodes one after another and its short height favorable for early flowering, made it possible to prepare many young inflorescence-bearing shoots with roots at one time. A simplified NSC cultivation system could also be developed at a lower cost, and by using it together with the NSC growth chamber, recycling of untreated materials was established for supplying steadily enough amounts of young inflorescence-bearing shoots with roots for mutagenicity testings. Compared with traditional methods of using potted plants or cuttings, the new method exhibited more stable flower production, better stamen-hair growth and a significantly lower spontaneous (background) mutation frequency, and could produce more inflorescences per space. The use of such young inflorescence-bearing shoots with roots was therefore judged to be satisfactory to serve as a new mutagenicity test system alternating with potted plants and cuttings.
Shima
N
N
Department of Regulation Biology, Faculty of Science, Saitama University, Urawa, Japan.
Xiao
L Z
LZ
Sakuramoto
F
F
Ichikawa
S
S
D002999:Clone Cells; D018527:Hydroponics / Q000379:methods*; D009152:Mutagenicity Tests / Q000379:methods; D009154:Mutation; D063245:Plant Development; D018517:Plant Roots / Q000235:genetics* / Q000502:physiology; D018520:Plant Shoots / Q000235:genetics* / Q000502:physiology; D010944:Plants / Q000235:genetics*