title: Cell kinetic changes in cultured tumor cells after treatment with radiation and chemotherapy.
abstract: Flow cytometry instantaneously determines the percentages of cells in various cell cycle phases to rapidly evaluate effects of irradiation and anti-cancer drugs. We studied these using growth curve analysis and 5-bromodeoxyuridine propidium iodine (BrdU)-(PI) double staining. With conventional DNA histogram methods, determination of S phase fraction was difficult because of overlapping DNA content between G1 and early S phases and between late S and G2 phases. Double staining directly differentiated G1, S, and G2 + M phases. By double staining, rapid transition from G1 to S occurred within 4 h after irradiation or after the drug treatments, and initial G1 arrest induced by irradiation was confirmed for the first time.
authors.affiliation: Department of Radiology, Kansai Medical University, 10-15 Fumizonocho, Moriguchi-city, Osaka, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Murata
authors.forename: K
authors.initials: K
authors.lastname: Akagi
authors.forename: M
authors.initials: M
authors.lastname: Uda
authors.forename: H
authors.initials: H
authors.lastname: Kimura
authors.forename: R
authors.initials: R
authors.lastname: Nasu
authors.forename: Y
authors.initials: Y
authors.lastname: Tanaka
mesh_terms: D000970:Antineoplastic Agents / Q000494:pharmacology*; D001761:Bleomycin / Q000494:pharmacology; D001973:Bromodeoxyuridine; D002453:Cell Cycle / Q000187:drug effects* / Q000528:radiation effects*; D003035:Cobalt / Q000737:chemistry; D004273:DNA, Neoplasm / Q000032:analysis; D004305:Dose-Response Relationship, Drug; D004317:Doxorubicin / Q000494:pharmacology; D005434:Flow Cytometry; D006367:HeLa Cells / Q000187:drug effects / Q000528:radiation effects; D006801:Humans; D007700:Kinetics; D011419:Propidium / Q000378:metabolism; D013936:Thymidine / Q000378:metabolism
chemical_list: D000970:Antineoplastic Agents; D004273:DNA, Neoplasm; D001761:Bleomycin; D011419:Propidium; D003035:Cobalt; D004317:Doxorubicin; D001973:Bromodeoxyuridine; D013936:Thymidine