Time-resolved fluorescence spectroscopy of a hematoporphyrin derivative used for photodynamic therapy of cancer.
Photodynamic therapy (PDT) is a treatment modality for malignancies that, uses the accumulation of a photosensitizer in tumor tissue and a subsequent laser light irradiation to selectively destroy tumor cells. Usually PDT is carried out with a hematoporphyrin derivative (HPD) as photosensitizer. Fluorescence characteristics of the HPD Photosan-3 were determined at different concentrations with time-resolved fluorescence spectroscopy. Photosan-3 showed a concentration dependent fluorescence with one fluorescence decay time below a concentration of 10 micrograms/ml, two or three decay times at 50 and 100 micrograms/ml Photosan-3 respectively. These findings confirm results of former studies on other HPD that aggregates and dimers are probably more responsible for the photodynamic effect than monomers.
Gottschlich
S
S
Department of Otorhinolaryngology, Head and Neck Surgery, Christian-Albrechts-University, Kiel, Germany.
Lippert
B M
BM
Schade
W
W
Werner
J A
JA
D005453:Fluorescence; D006415:Hematoporphyrins; D007834:Lasers; D009369:Neoplasms / Q000628:therapy*; D010778:Photochemotherapy; D017319:Photosensitizing Agents / Q000627:therapeutic use*; D013050:Spectrometry, Fluorescence; D013997:Time Factors
D006415:Hematoporphyrins; D017319:Photosensitizing Agents; C071875:photosan III