[Glutathione, a biochemical indicator for different types of cellular stress. The change in the GSH level in the brain of rats with hypoxia].
By the ligation of left vertebral artery in some white, adult WISTAR rats, of 150-200 g, anaesthetized with ether, a cerebral hypoxia of 10 minutes was induced. After beheading, there was determined the level of SH-neproteic groups and glutathione (GSH) in the whole heparinized blood and there was observed an important decrease with -15.88% and -16.80% respectively, in comparison with the normal lot (100%), kept under the some laboratory conditions. The GSH level in the neural cytosol is also significantly decreased with -8.66% in rats with hypoxia. The GSH depletion in the induced hypoxia, by our experiment, might be due to the reaction between nitric oxide (NO.) radical and intracellular GSH (blood and neuronal cytosol), considering the literature in this field, and S-nitrosoglutathion (S-NO-GSH) bioactive intermediary is formed and it protects the hypoxied brain against the NO.-dependent cytotoxicity, in the initial phase of hypoxia. The decrease of GSH and SH-neproteic groups level is considered a biochemical marker of the growth of the free radicals level to O2 (NO., O2., OH., H2O2, 1O2), that appear in the hypoxic and/or ischemic stress, but also a major sign in the rol of "scavenger" and antioxidant of the radicals at the cerebral and sanguine level, in the above mentioned conditions.
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Disciplina de Biochimie, Universitatea de Medicină şi Farmacie Gr. T. Popa, Iaşi.
Busuioc
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Nastasia
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Haulică
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D000818:Animals; D015415:Biomarkers; D001921:Brain / Q000378:metabolism*; D003600:Cytosol / Q000378:metabolism; D005978:Glutathione / Q000378:metabolism*; D000860:Hypoxia / Q000378:metabolism*; D008297:Male; D009474:Neurons / Q000378:metabolism; D010084:Oxidation-Reduction; D018384:Oxidative Stress / Q000502:physiology*; D051381:Rats; D017208:Rats, Wistar; D013438:Sulfhydryl Compounds / Q000378:metabolism
D015415:Biomarkers; D013438:Sulfhydryl Compounds; D005978:Glutathione
Glutationul, indicator biochimic pentru diferite tipuri de stress celular. Modificarea nivelului GSH-ului în creierul de şobolani cu hipoxie.