Recombinant human interleukin-11 does not modify biochemical parameters of bone remodeling and bone mineral density in adult ovariectomized rats.
Interleukin (IL)-11 stimulates osteoclast formation and inhibits osteoblast function in vitro and has been implicated in estrogen deficiency-induced bone loss. Herein we report the in vivo effect of recombinant human IL-11 (rHU-IL-11), administered s.c. in doses between 10 and 200 microg/kg/day for 6 weeks into 6-month-old rats after ovariectomy. There was no difference between vehicle-treated and rHu-IL-11 treated rats in the ovariectomy-induced increase in the urinary excretion of pyridinoline and deoxypyridinoline. Neither was there a significant effect of rHu-IL-11 on the plasma concentrations of osteocalcin and on bone mineral density (BMD) measured at a metaphyseal area of the distal femur after 6 weeks. At all dosages tested, rHu-IL-11 increased the femoral diaphyseal area. In conclusion, IL-11 has no deleterious in vivo effect on biochemical parameters of bone remodeling and BMD in estrogen-deficient rats.
Verhaeghe
J
J
Department of Obstetrics and Gynecology, Bone Disease Research Unit, Katholieke Universiteit Leuven, Belgium.
Van Herck
E
E
van Bree
R
R
Bouillon
R
R
Dequeker
J
J
Keith
J C
JC
D000596:Amino Acids / Q000378:metabolism / Q000652:urine; D000818:Animals; D015415:Biomarkers / Q000097:blood* / Q000652:urine*; D001835:Body Weight / Q000187:drug effects; D015519:Bone Density / Q000187:drug effects*; D016723:Bone Remodeling / Q000187:drug effects*; D001842:Bone and Bones / Q000187:drug effects / Q000502:physiology; D002118:Calcium / Q000652:urine; D004305:Dose-Response Relationship, Drug; D005260:Female; D006801:Humans; D017370:Interleukin-11 / Q000008:administration & dosage / Q000494:pharmacology*; D007668:Kidney / Q000254:growth & development; D009929:Organ Size / Q000187:drug effects; D015675:Osteocalcin / Q000097:blood; D010024:Osteoporosis / Q000503:physiopathology; D010052:Ovariectomy; D051381:Rats; D017208:Rats, Wistar; D011994:Recombinant Proteins / Q000008:administration & dosage / Q000494:pharmacology; D013154:Spleen / Q000254:growth & development
D000596:Amino Acids; D015415:Biomarkers; D017370:Interleukin-11; D011994:Recombinant Proteins; D015675:Osteocalcin; C015484:pyridinoline; D002118:Calcium