Passive protection of neonatal calves against bovine coronavirus-induced diarrhea by administration of egg yolk or colostrum antibody powder.
The protective effect of egg yolk and colostrum powders prepared from hens and cows vaccinated with inactivated bovine coronavirus (BCV) antigen was evaluated in a challenge model with a virulent BCV strain. Twenty three calves from BCV-free herds were randomly divided into control and several treatment groups. All calves were orally challenged with 1 x 10(9) TCID50 of the virulent Kakegawa strain of BCV at 24 to 36 h after birth. Calves in treatment groups received either egg yolk powder or cow colostrum containing BCV specific antibodies. Daily treatment with these antibody preparations started 6 h until 7 days post-challenge. Control calves which received no antibody had severe diarrhea and all died within 6 days after infection. In contrast, calves fed milk containing egg yolk or colostrum with neutralization titers of 1:2560 or 1:10,240 respectively all survived and had positive weight gain unlike the other treatment groups. These results indicate that the orally administered egg yolk and colostrum powders protected against BCV-induced diarrhea in neonatal calves and that the egg yolk used provided a higher degree of protection compared to colostrum powder on a titer basis. Treatment with whole egg yolk from immunized hens therefore provides a more efficacious alternative to the existing methods of specific passive protection against BCV.
Ikemori
Y
Y
Ghen Corporation Immunology Research Institute, Gifu City, Japan.
Ohta
M
M
Umeda
K
K
Icatlo
F C
FC
Kuroki
M
M
Yokoyama
H
H
Kodama
Y
Y
D000818:Animals; D000914:Antibodies, Viral / Q000008:administration & dosage*; D000918:Antibody Specificity; D002417:Cattle; D002418:Cattle Diseases; D003126:Colostrum / Q000821:virology; D018352:Coronavirus Infections / Q000276:immunology / Q000517:prevention & control / Q000662:veterinary*; D017938:Coronavirus, Bovine / Q000276:immunology; D003967:Diarrhea / Q000517:prevention & control / Q000662:veterinary* / Q000821:virology; D004530:Egg Yolk; D007116:Immunization, Passive / Q000379:methods / Q000662:veterinary*; D009500:Neutralization Tests
D000914:Antibodies, Viral