Cape Town solution in prolonged myocardial preservation: structural and ultrastructural study.
This study deals with myocardial preservation after 24 hours of continuous, hypothermic and oxygenated perfusion with Cape Town (CT) solution, focusing on the morphological changes produced by preservation and reperfusion, and their possible relationship to the composition of the solution and the immediate hemodynamic findings after orthotopic heart transplantation in dogs. After preservation, aside from mild or moderate mitochondrial changes, the most relevant lesions included edema and vasoconstriction. Reperfusion was followed by the development of areas of necrosis forming contraction bands and an increment in the mitochondrial damage; the intercalated disks conserved their normal structure; edema became more prominent and was invariably accompanied by hemorrhage; vasoconstriction was very pronounced and was accompanied on occasion by evidence of capillary rupture; and inflammatory cells were observed in the interstitium. These results indicate that colloid must be added to Cape Town solution and that reperfusion probably requires selective approaches to deal with vasoconstriction and inflammation.
García-Poblete
E
E
Department of Cellular Biology, Complutensis University of Madrid, Spain.
Alvarez
L
L
Fernández
H
H
Escudero
C
C
Torralba
A
A
D000818:Animals; D003402:Creatine Kinase / Q000378:metabolism; D004285:Dogs; D006321:Heart / Q000187:drug effects; D006863:Hydrogen-Ion Concentration; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D019344:Lactic Acid / Q000378:metabolism; D008854:Microscopy, Electron; D009206:Myocardium / Q000378:metabolism / Q000648:ultrastructure; D009926:Organ Preservation / Q000379:methods*; D019188:Organ Preservation Solutions / Q000494:pharmacology*
D019188:Organ Preservation Solutions; D019344:Lactic Acid; D007770:L-Lactate Dehydrogenase; D003402:Creatine Kinase