A hypersensitive response-like mechanism is involved in resistance of potato plants bearing the Ry(sto) gene to the potyviruses potato virus Y and tobacco etch virus.
Potato plants carrying the Ry(sto) gene from Solanum stoloniferum are extremely resistant to a number of potyviruses, but it is not known at what stage of infection the resistance is expressed. The resistance may be due to Ry(sto) or to a closely linked gene. In this investigation, we used potato virus Y (PVY) and a tobacco etch virus construct that encodes beta-glucuronidase (TEV-GUS) to monitor virus infections of potato plants. Systemic spread of either virus in resistant potato plants was not detectable by serology, RT-PCR, GUS assay or bioassay although each replicated in the initially infected cells of leaves from resistant potato cultivars and was transported into neighbouring cells. However, 3 days post-inoculation (p.i.) a necrotic reaction set in that stopped movement and accumulation of both viruses by 7 days p.i. The resistance reaction (probably a hypersensitive reaction) became visible as necrotic streaks on veins on the lower leaflet surfaces of some potato cultivars carrying the Ry(sto) gene and may be elicited by a common potyviral gene product.
Hinrichs
J
J
University of Kentucky, College of Agriculture, Plant Pathology, Lexington 40546-0091, USA. hinrichs@uni-hohenheim.de
Berger
S
S
Shaw
J G
JG
D017343:Genes, Plant; D010935:Plant Diseases / Q000821:virology; D017800:Potyvirus / Q000276:immunology*; D011198:Solanum tuberosum / Q000276:immunology*