Interaction of dehydroepiandrosterone with phospholipid membranes: an infrared spectroscopy investigation.
The interaction between dehydroepiandrosterone (DHEA) and its sulfate metabolite (DHEA-S) with deuterated dimirystoylphosphatidylcholine (DMPC-d54) was investigated by FTIR spectroscopy. DHEA, as cholesterol, induces some conformational order in the liquid-crystalline phase of DMPC-d54. Attenuated total reflectance (ATR) measurements performed on oriented DMPC-d54/steroids samples have shown that in the gel phase, the acyl chains of DMPC-d54 become more normal to the bilayer surface in the presence of DHEA or cholesterol. On the other hand, DHEA-S increases the number of gauche conformers along the hydrocarbon chains of DMPC-d54. No evidence for the presence of hydrogen bond was found between both steroids and the 13C labeled carbonyl group of hydrated DMPC.
Dicko
A
A
Département de Chimie and Centre de Recherche en Sciences et Ingénierie des Macromolécules, Université Laval, Sainte-Foy, Québec, Canada.
Di Paolo
T
T
Pézolet
M
M
D002784:Cholesterol / Q000737:chemistry; D003687:Dehydroepiandrosterone / Q000737:chemistry*; D019314:Dehydroepiandrosterone Sulfate / Q000737:chemistry*; D004134:Dimyristoylphosphatidylcholine / Q000737:chemistry*; D008051:Lipid Bilayers / Q000737:chemistry*; D008081:Liposomes / Q000737:chemistry; D008968:Molecular Conformation; D017550:Spectroscopy, Fourier Transform Infrared
D008051:Lipid Bilayers; D008081:Liposomes; D003687:Dehydroepiandrosterone; D019314:Dehydroepiandrosterone Sulfate; D002784:Cholesterol; D004134:Dimyristoylphosphatidylcholine