title: A histological and immunohistochemical study on chronic irritant contact dermatitis.
abstract: BACKGROUND
  Chronic irritant contact dermatitis (CICD) is characterized by erythema, scaling, hyperkeratosis, chapping and fissures. It may be the result of skin damage evoked by the cumulative effect of a variety of irritant stimuli. The diagnosis of CICD is made on basis of the patient's history and clinical features. No specific diagnostic tests are available.
  
  
  OBJECTIVE
  The histopathologic and cell biological features of CICD have not been extensively studied. Here, we describe the histological and immunohistological changes in CICD.
  
  
  METHODS
  Punch biopsies were taken from 11 patients with CICD for hematoxylin eosin and immunohistochemical stainings. Four skin biopsies of the palms of the hands of healthy volunteers served as controls.
  
  
  RESULTS
  The histopathologic pattern was characterized by different grades of hyperkeratosis, parakeratosis, spongiosis, exocytosis, acanthosis, and mononuclear perivascular infiltrates. Mitotic activity, as measured by Ki-67-staining in the epidermis, was increased fourfold in involved skin as compared with normal skin. Involucrin, a structural protein of the cornified envelope, was expressed from the stratum granulosum throughout the stratum spinosum in all patients with CICD and was upregulated in comparison with normal skin. Epidermal fatty-acid binding protein (E-FABP), a terminal differentiation marker, was proportionally unaltered in the CICD as compared with the normal skin and was localized from the stratum granulosum to the upper layers of the stratum spinosum. Cytokeratin 16, a differentiation marker expressed in hyperproliferative epidermis, was markedly increased from the stratum granulosum throughout the stratum spinosum in 5 out of 11 patients with CICD. Skin-derived antiproteinase (SKALP)/elafin, a proteinase inhibitor expressed in inflamed epidermis, was only detected within the stratum granulosum in 3 out of 11 patients.
  
  
  CONCLUSIONS
  We conclude that CICD is clinically characterized by features of a chronic dermatitis and, at the histological level, by inflammatory changes, epidermal hyperproliferation and altered differentiation.
authors.affiliation: Department of Dermatology, University Hospital Nijmegen, The Netherlands.
authors.forename: T K
authors.initials: TK
authors.lastname: Le
authors.forename: J
authors.initials: J
authors.lastname: Schalkwijk
authors.forename: P C
authors.initials: PC
authors.lastname: van de Kerkhof
authors.forename: U
authors.initials: U
authors.lastname: van Haelst
authors.forename: P G
authors.initials: PG
authors.lastname: van der Valk
mesh_terms: D000293:Adolescent; D000328:Adult; D002352:Carrier Proteins / Q000032:analysis; D002455:Cell Division; D002908:Chronic Disease; D017453:Dermatitis, Irritant / Q000378:metabolism / Q000473:pathology*; D000071438:Fatty Acid-Binding Protein 7; D050556:Fatty Acid-Binding Proteins; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D008297:Male; D008875:Middle Aged; D018996:Myelin P2 Protein / Q000032:analysis; D009363:Neoplasm Proteins; D011498:Protein Precursors / Q000032:analysis; D053491:Proteinase Inhibitory Proteins, Secretory; D011506:Proteins / Q000032:analysis; D012867:Skin / Q000737:chemistry / Q000473:pathology*; D025521:Tumor Suppressor Proteins
chemical_list: D002352:Carrier Proteins; C493412:FABP5 protein, human; C107608:FABP7 protein, human; D000071438:Fatty Acid-Binding Protein 7; D050556:Fatty Acid-Binding Proteins; D018996:Myelin P2 Protein; D009363:Neoplasm Proteins; D011498:Protein Precursors; D053491:Proteinase Inhibitory Proteins, Secretory; D011506:Proteins; D025521:Tumor Suppressor Proteins; C032253:involucrin