Synthesis and biodistribution of a short nonionic oligonucleotide analogue in mouse with a potential to mimic peptides.
A nonionic RNA analogue of the sequence r(USO2GSO2ASO2C) has been synthesized where each bridging phosphate diester is replaced by a dimethylene sulfone unit (rSNA). The rSNA was synthesized in solution from 3',5'-bishomo-beta-ribonucleoside derivatives as building blocks. Full experimental procedures are provided, and the product and all synthetic intermediates are fully characterized. The tetramer is nonionic but highly dipolar due to multiple hydrogen bonding opportunities. It is freely soluble in water only at higher pH's, permitting it to be radiolabeled by exchange of the acidic protons alpha to the sulfones with tritiated water. The tritiated molecule was administered intravenously into the tail vein (2.6 mg/kg) of mice, and its distribution was monitored over 48 h. The rSNA was widely distributed in the biological tissues, including the brain, and excreted in both the feces and the urine. The accumulation of radioactivity was significantly higher in liver and kidney than in other tissues. Radiolabel was recovered from the urine, analyzed by HPLC, and shown to be intact oligonucleotide sulfone. This is the first bioavailability study on a short nonionic oligonucleotide analogue, a class of molecules with potential biomedical applications.
Eschgfäller
B
B
Department of Chemistry, Swiss Federal Institute of Technology, Zurich, Switzerland.
König
M
M
Boess
F
F
Boelsterli
U A
UA
Benner
S A
SA
D000818:Animals; D009682:Magnetic Resonance Spectroscopy; D051379:Mice; D018716:Molecular Mimicry; D015394:Molecular Structure; D009841:Oligonucleotides / Q000138:chemical synthesis* / Q000493:pharmacokinetics; D010455:Peptides / Q000493:pharmacokinetics*; D016339:Spectrometry, Mass, Fast Atom Bombardment; D014018:Tissue Distribution
D009841:Oligonucleotides; D010455:Peptides