title: Upregulation of neuronal NOS mRNA in the PVN and SON of inherited diabetes insipidus rats.
abstract: We investigated the expression of neuronal nitric oxide synthase (nNOS) gene in the paraventricular (PVN) and supraoptic nuclei (SON) of rats with inherited diabetes insipidus (DI), using in situ hybridization histochemistry. The DI rats showed hypo-osmotic polyuria and polydipsia with arginine vasopressin (AVP) deficiency. The expression of nNOS gene in the PVN and SON in homozygous (di/di) rats was significantly increased in comparison to normal Wistar and heterozygous (di/+) rats. nNOS gene-expressing cells were distributed throughout the PVN and SON, including the divisions of AVP and oxytocin gene expressing cells in di/di rats. These results suggest that the expression of nNOS gene is upregulated in the magnocellular neurons in the PVN and SON of inherited DI rats.
authors.affiliation: Department of Physiology, University of Occupational and Environmental Health, School of Medicine, Kitakyushu, Japan.
authors.forename: Y
authors.initials: Y
authors.lastname: Yamamoto
authors.forename: Y
authors.initials: Y
authors.lastname: Ueta
authors.forename: M
authors.initials: M
authors.lastname: Nomura
authors.forename: R
authors.initials: R
authors.lastname: Serino
authors.forename: N
authors.initials: N
authors.lastname: Kabashima
authors.forename: I
authors.initials: I
authors.lastname: Shibuya
authors.forename: H
authors.initials: H
authors.lastname: Yamashita
mesh_terms: D000818:Animals; D003919:Diabetes Insipidus / Q000235:genetics / Q000378:metabolism*; D015870:Gene Expression; D006651:Histocytochemistry; D017403:In Situ Hybridization; D009474:Neurons / Q000378:metabolism*; D019001:Nitric Oxide Synthase / Q000235:genetics*; D010286:Paraventricular Hypothalamic Nucleus / Q000378:metabolism*; D012333:RNA, Messenger / Q000378:metabolism*; D051381:Rats; D011910:Rats, Brattleboro; D017208:Rats, Wistar; D013495:Supraoptic Nucleus / Q000378:metabolism*; D015854:Up-Regulation
chemical_list: D012333:RNA, Messenger; D019001:Nitric Oxide Synthase