Antimicrobial durability and rare ultrastructural colonization of indwelling central catheters coated with minocycline and rifampin.
OBJECTIVE
To determine the duration of antimicrobial activity and the efficacy of indwelling catheters coated with minocycline and rifampin in preventing ultrastructural colonization.


METHODS
Multicenter, prospective, randomized, clinical trial.


METHODS
Five university-based medical centers.


METHODS
Cohort 1 consisted of 40 randomized patients in whom an equal number of minocycline- and rifampin-coated and uncoated catheters were inserted and studied using scanning electron microscopy. Cohort 2 consisted of 118 patients who received coated catheters that were tested for the antimicrobial activity and levels of the antibiotics at the time of removal.


METHODS
Catheters pretreated with tridodecylmethylammonium chloride and subsequently coated with minocycline and rifampin; uncoated catheters (control).


RESULTS
Quantitative scanning electron microscopy was utilized to determine both the ultrastructural colonization in biofilm on coated and uncoated catheters. The zones of inhibition of coated catheters from studied patients against Staphylococcus epidermidis was used to determine the antimicrobial durability. High-performance liquid chromatography was used to determine antibiotic levels on indwelling coated catheters and in serum. Mild-to-heavy ultrastructural colonization was detected in 7 (35%) of 20 coated catheters and in 16 (80%) of 20 uncoated catheters (p = .004). Significant antimicrobial inhibitory activity against S. epidermidis was maintained for 16 days. Rifampin and minocycline continued to be detected on the surfaces of coated catheters for at least 2 wks after placement. Neither antibiotic was detected in the 60 serum samples obtained from 15 patients during catheterization.


CONCLUSIONS
Coating catheters with minocycline and rifampin inhibits ultrastructural colonization of indwelling catheters and maintains effective antimicrobial activity for at least 2 wks.
Raad
I I
II
University of Texas M. D. Anderson Cancer Center, Section of Infectious Diseases, Houston 77030, USA.
Darouiche
R O
RO
Hachem
R
R
Abi-Said
D
D
Safar
H
H
Darnule
T
T
Mansouri
M
M
Morck
D
D
D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000900:Anti-Bacterial Agents / Q000008:administration & dosage*; D000904:Antibiotics, Antitubercular / Q000008:administration & dosage*; D002405:Catheterization, Central Venous / Q000295:instrumentation*; D002408:Catheters, Indwelling / Q000382:microbiology; D004355:Drug Stability; D004866:Equipment Contamination / Q000517:prevention & control*; D005260:Female; D006801:Humans; D008297:Male; D008855:Microscopy, Electron, Scanning; D008875:Middle Aged; D008911:Minocycline / Q000008:administration & dosage*; D011446:Prospective Studies; D012293:Rifampin / Q000008:administration & dosage*; D013781:Texas; D013997:Time Factors
D000900:Anti-Bacterial Agents; D000904:Antibiotics, Antitubercular; D008911:Minocycline; D012293:Rifampin