[Metabolic and rheologic changes in long-term hypothermia of erythrocyte concentrates over 15 weeks].
With a multiple washing procedure in a chloride ion-free citrate-phosphate-glucose-adenine additive solution or with storage of red blood cells in a large volume of this solution, erythrocytes can be stored for 15 weeks at 4 degrees C. After 12 weeks' storage in this chloride-free solution the red blood cell parameters (2,3-DPG, glucose, lactose) measured are as good as stored erythrocytes resuspended in SAG-Mannitol after 21 days. Rheological parameters (morphology, filterability) show a maintenance of deformability of such preserved cells up to 84 days of storage. The preservation of erythrocytes by this method has the advantage that beside the hypothermic long-term storage (potential second aHIV screening of blood donors) the blood is of better quality in the first weeks of storage.
Matthes
G
G
Institut für Transfusionsmedizin, Universitätsklinikum Charité, Humboldt-Universität zu Berlin, Deutschland.
Richter
E
E
Tofoté
U
U
Kucera
W
W
Lerche
D
D
D019794:2,3-Diphosphoglycerate / Q000097:blood; D001786:Blood Glucose / Q000378:metabolism; D001793:Blood Preservation / Q000379:methods*; D003080:Cold Temperature; D016238:Cytapheresis; D004907:Erythrocyte Deformability; D017707:Erythrocyte Transfusion; D004912:Erythrocytes / Q000166:cytology* / Q000502:physiology*; D006801:Humans; D007773:Lactates / Q000097:blood; D012212:Rheology; D013997:Time Factors
D001786:Blood Glucose; D007773:Lactates; D019794:2,3-Diphosphoglycerate
Metabolische und rheologische Veränderungen bei der hypothermen Langzeitlagerung von Erythrozytenkonzentraten über 15 Wochen.