title: Antibodies against EMC virus RNA-VPg recognize Tyr-(5'P-->O)-pU and immunostain infected cells.
abstract: Covalent complexes of nucleic acids and proteins are widespread among viruses. Covalent complexes of RNA and proteins are proposed to exist in eukaryotic cells. The goal of this work was to obtain specific antibodies to the covalent linkage unit (CLU) between virus RNA and protein to search cellular RNA-protein complexes. Antibodies were generated by direct immunization of a rabbit with the BSA-coupled EMC virus RNA-VPg complex. By a dot-blot immunoassay and immunofluorescent microscopy it was found that the antibodies specifically recognize both EMC virus RNA-VPg and synthetic CLU-containing compounds. Thus, a fraction of the antibodies was directed to CLU.
authors.affiliation: A.N. Belozersky Institute of Physico-Chemical Biology, Moscow State University, Russia.
authors.forename: O A
authors.initials: OA
authors.lastname: Bordunova
authors.forename: O V
authors.initials: OV
authors.lastname: Turina
authors.forename: E S
authors.initials: ES
authors.lastname: Nadezhdina
authors.forename: G S
authors.initials: GS
authors.lastname: Shatskaya
authors.forename: V P
authors.initials: VP
authors.lastname: Veiko
authors.forename: Y F
authors.initials: YF
authors.lastname: Drygin
mesh_terms: D000818:Animals; D000906:Antibodies / Q000276:immunology* / Q000378:metabolism; D016466:CHO Cells / Q000166:cytology / Q000276:immunology / Q000821:virology; D006224:Cricetinae; D004686:Encephalomyocarditis virus / Q000737:chemistry* / Q000276:immunology; D005455:Fluorescent Antibody Technique; D015151:Immunoblotting; D009843:Oligoribonucleotides / Q000276:immunology*; D019000:Phosphotyrosine / Q000031:analogs & derivatives / Q000276:immunology; D012367:RNA, Viral / Q000276:immunology / Q000378:metabolism; D012709:Serum Albumin / Q000276:immunology; D014443:Tyrosine / Q000276:immunology* / Q000378:metabolism; D014758:Viral Core Proteins / Q000276:immunology*; D014764:Viral Proteins / Q000276:immunology / Q000378:metabolism
chemical_list: D000906:Antibodies; D009843:Oligoribonucleotides; D012367:RNA, Viral; D012709:Serum Albumin; D014758:Viral Core Proteins; D014764:Viral Proteins; D019000:Phosphotyrosine; D014443:Tyrosine