Attachment and growth of cultured fibroblast cells on PVA/chitosan-blended hydrogels.
Hydrogels were prepared from poly(vinyl alcohol) and chitosan in various blend ratios. The water contents of the hydrogels were in the range of 65 to 75 wt %. The attachment and growth of fibroblast cells (L-929) on the hydrogels were studied with a cell culture method. On the hydrogels with more than 15 wt % chitosan content, the attached cells were able not only to remain viable but also to proliferate. The relative cell attachment after incubation for 30 h increased with increasing chitosan content in the hydrogels. Cell attachment and growth on the hydrogel with 40 wt % chitosan content exceeded those on collagen, a widely-used mammalian cell culture substrate. The morphology of the cells attached onto the hydrogels with a lower chitosan content was spherical, but in hydrogels with more than 15 wt % chitosan content, the number of spindle-shaped cells increased with increasing chitosan content.
Koyano
T
T
National Institute of Materials and Chemical Research, Tsukuba, Ibaraki, Japan.
Minoura
N
N
Nagura
M
M
Kobayashi
K
K
D001672:Biocompatible Materials; D002478:Cells, Cultured; D002686:Chitin / Q000031:analogs & derivatives*; D048271:Chitosan; D004548:Elasticity; D005347:Fibroblasts; D005782:Gels; D006801:Humans; D011142:Polyvinyl Alcohol; D014867:Water / Q000737:chemistry
D001672:Biocompatible Materials; D005782:Gels; D014867:Water; D002686:Chitin; D011142:Polyvinyl Alcohol; D048271:Chitosan