title: Human DRA functions as a sulfate transporter in Sf9 insect cells.
abstract: DRA is a gene that is down-regulated in colon adenomas and adenocarcinomas in humans. We have previously shown that DRA proteins are found as various forms in tissue due to differential glycosylation. This study has focused on the function of DRA related to its subcellular localization. We used the baculovirus expression system and overexpressed a nearly full-length DRA driven by a polyhedrin promoter in Sf9 insect cells. DRA protein expressed in this cell was underglycosylated relative to normal colon mucosa, but uniformly targeted to the cell membrane. It also appears to undergo posttranslational cleavage, removing about 100 amino acids from its amino terminus. This membrane localization is similar to what we observed in the colon mucosa. An ion transport assay demonstrated that DRA functions as a sulfate transporter. When DRA was expressed, sulfate import was increased more than threefold compared to the control. Sulfate import was inhibitable by the anion transporter inhibitor, DIDS, in a dose-dependent fashion. Given that (1) DRA has high similarity to other identified sulfate transporters and the proposed structure of DRA polypeptide is characteristic of those transporters, (2) DRA localization is limited to the cell membrane, and (3) DRA expression correlates with intestinal differentiation in mouse, we suggest that DRA represents a tissue-specific member of the sulfate transporter family.
authors.affiliation: Division of Hematology/Oncology, Medical University of South Carolina, Charleston, South Carolina 29425, USA.
authors.forename: M K
authors.initials: MK
authors.lastname: Byeon
authors.forename: A
authors.initials: A
authors.lastname: Frankel
authors.forename: T S
authors.initials: TS
authors.lastname: Papas
authors.forename: K W
authors.initials: KW
authors.lastname: Henderson
authors.forename: C W
authors.initials: CW
authors.lastname: Schweinfest
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D017920:Antiporters; D016367:Baculoviridae / Q000235:genetics; D001483:Base Sequence; D002352:Carrier Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D002460:Cell Line; D002462:Cell Membrane / Q000378:metabolism; D027963:Chloride-Bicarbonate Antiporters; D003106:Colon / Q000378:metabolism; D018076:DNA, Complementary / Q000235:genetics; D015870:Gene Expression; D006031:Glycosylation; D006801:Humans; D008565:Membrane Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D026901:Membrane Transport Proteins; D051379:Mice; D008969:Molecular Sequence Data; D011499:Protein Processing, Post-Translational; D018411:Spodoptera; D000076988:Sulfate Transporters; D013431:Sulfates / Q000378:metabolism*
chemical_list: D017920:Antiporters; D002352:Carrier Proteins; D027963:Chloride-Bicarbonate Antiporters; D018076:DNA, Complementary; D008565:Membrane Proteins; D026901:Membrane Transport Proteins; C081011:SLC26A3 protein, human; C085609:SLC26A4 protein, human; C489860:Slc26a3 protein, mouse; D000076988:Sulfate Transporters; D013431:Sulfates