Effect of human neutrophil elastase on tracheal mucociliary transport in anesthetized quails.
We investigated the effect of human neutrophil elastase (HNE) on tracheal mucociliary transport in anesthetized quails. Topical application of HNE (30-300 microg/kg) to tracheal mucosa dose-dependently decreased mucociliary transport velocity (MCTV). The HNE (300 microg/kg)-induced decrease in MCTV was blocked by ONO-5046 x Na (sodium N-[2-[4-(2,2-dimethylpropionyloxy)phenyl-sulfonylamino]benzo yl]aminoacetate tetrahydrate) (3-30 mg/kg, i.m.), a specific neutrophil elastase inhibitor. Furthermore, we found that HNE increased DNA, fucose and protein contents of tracheal lavages, and the increases were also reverted by ONO-5046 Na. These results indicated that HNE decreased tracheal mucociliary transport, and the decrease may be, at least in part, ascribed to the deterioration of tracheal secretions.
Tai
S
S
Department of Pharmacological Sciences, Faculty of Pharmaceutical Sciences, Kumamoto University, Japan.
Kai
H
H
Kido
T
T
Isohama
Y
Y
Takahama
K
K
Miyata
T
T
D000818:Animals; D001992:Bronchoalveolar Lavage Fluid / Q000737:chemistry; D004247:DNA / Q000032:analysis; D004305:Dose-Response Relationship, Drug; D005643:Fucose / Q000032:analysis; D005998:Glycine / Q000031:analogs & derivatives* / Q000494:pharmacology; D006801:Humans; D019272:Leukocyte Elastase / Q000037:antagonists & inhibitors / Q000494:pharmacology*; D008297:Male; D009079:Mucociliary Clearance / Q000187:drug effects*; D009092:Mucous Membrane / Q000187:drug effects / Q000378:metabolism; D011506:Proteins / Q000032:analysis; D011784:Quail; D015842:Serine Proteinase Inhibitors / Q000494:pharmacology*; D013449:Sulfonamides / Q000494:pharmacology*; D014132:Trachea / Q000187:drug effects* / Q000378:metabolism
D011506:Proteins; D015842:Serine Proteinase Inhibitors; D013449:Sulfonamides; D005643:Fucose; D004247:DNA; C069195:sivelestat; D019272:Leukocyte Elastase; D005998:Glycine