title: Distinct cellular interactions of secreted and transmembrane Ebola virus glycoproteins.
abstract: The mechanisms by which Ebola virus evades detection and infects cells to cause hemorrhagic fever have not been defined, though its glycoprotein, synthesized in either a secreted or transmembrane form, is likely involved. Here the secreted glycoprotein was found to interact with neutrophils through CD16b, the neutrophil-specific form of the Fc gamma receptor III, whereas the transmembrane glycoprotein was found to interact with endothelial cells but not neutrophils. A murine retroviral vector pseudotyped with the transmembrane glycoprotein preferentially infected endothelial cells. Thus, the secreted glycoprotein inhibits early neutrophil activation, which likely affects the host response to infection, whereas binding of the transmembrane glycoprotein to endothelial cells may contribute to the hemorrhagic symptoms of this disease.
authors.affiliation: Howard Hughes Medical Institute and Department of Internal Medicine, University of Michigan, Ann Arbor, MI 48109, USA.
authors.forename: Z
authors.initials: Z
authors.lastname: Yang
authors.forename: R
authors.initials: R
authors.lastname: Delgado
authors.forename: L
authors.initials: L
authors.lastname: Xu
authors.forename: R F
authors.initials: RF
authors.lastname: Todd
authors.forename: E G
authors.initials: EG
authors.lastname: Nabel
authors.forename: A
authors.initials: A
authors.lastname: Sanchez
authors.forename: G J
authors.initials: GJ
authors.lastname: Nabel
mesh_terms: D002460:Cell Line; D029043:Ebolavirus / Q000235:genetics / Q000378:metabolism / Q000472:pathogenicity* / Q000502:physiology; D004730:Endothelium, Vascular / Q000166:cytology / Q000378:metabolism* / Q000821:virology; D005814:Genes, Viral; D005822:Genetic Vectors; D006023:Glycoproteins / Q000235:genetics / Q000378:metabolism*; D019142:Hemorrhagic Fever, Ebola / Q000821:virology; D006801:Humans; D019041:L-Selectin / Q000378:metabolism; D008562:Membrane Glycoproteins / Q000235:genetics / Q000378:metabolism*; D008979:Moloney murine leukemia virus / Q000235:genetics / Q000502:physiology; D018375:Neutrophil Activation; D009504:Neutrophils / Q000276:immunology / Q000378:metabolism*; D017452:Receptors, IgG / Q000378:metabolism; D014162:Transfection; D014407:Tumor Cells, Cultured; D014763:Viral Matrix Proteins / Q000235:genetics / Q000378:metabolism*; D014764:Viral Proteins / Q000235:genetics / Q000378:metabolism*
chemical_list: D006023:Glycoproteins; D008562:Membrane Glycoproteins; D017452:Receptors, IgG; D014763:Viral Matrix Proteins; D014764:Viral Proteins; C110613:secreted glycoprotein, Ebola virus; C110614:transmembrane glycoprotein, Ebola virus; D019041:L-Selectin