title: Polypeptide pattern of mRNA isolated from polysomal and non-polysomal mRNA-protein(mRNP) fractions in rat myoblast.
abstract: Polypeptides synthesized from polysome-bound and unbound fractions of polyA mRNA were studied by in-vivo cells labelling and cDNA-mRNA hybrid-arrest translation. The polypeptides synthesized from unbound(free) fraction appeared to be significantly reduced in the absence of cycloheximide in L6 myoblast. The cDNA excess-mRNA hybrid-arrest translation exhibited rare polypeptide sequences in the unbound fractions.
authors.affiliation: Department of Medicine, Memorial University of Newfoundland, St. John's, Canada.
authors.forename: P
authors.initials: P
authors.lastname: Bhattacharya
mesh_terms: D000818:Animals; D005591:Chemical Fractionation; D009132:Muscles / Q000378:metabolism*; D010455:Peptides / Q000032:analysis*; D011132:Polyribosomes / Q000737:chemistry*; D012333:RNA, Messenger / Q000737:chemistry* / Q000302:isolation & purification; D051381:Rats; D012261:Ribonucleoproteins / Q000378:metabolism*
chemical_list: D010455:Peptides; D012333:RNA, Messenger; D012261:Ribonucleoproteins