A contiguous 60 kb genomic stretch from barley reveals molecular evidence for gene islands in a monocot genome.
The contiguous DNA sequence of a 60 kb genomic interval of barley chromosome 4HL has been assembled. The region harbours a single and novel gypsy -like retrotransposon, designated BAGY-1. Only three genes appear to reside in the genomic stretch. One predicts a plant homologue of ribophorin I, a subunit of the oligosaccharyltransferase-protein complex located in the rough endoplasmatic reticulum. The second is similar to the Drosophila g1 gene encoding a ring finger protein involved in developmental processes. The observed gene density is approximately 5-fold lower than in the best characterized dicot genome of Arabidopsis but 6- to 10-fold higher than expected from an equidistant gene distribution in the complex barley genome. Our data suggest that the 60 kb genomic interval represents part of a gene island, a seemingly distinctive feature of grass genomes.
Panstruga
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The Sainsbury Laboratory, John Innes Centre, Norwich Research Park, Colney Lane, Norwich NR4 7UH, UK.
Büschges
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Piffanelli
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Schulze-Lefert
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D000595:Amino Acid Sequence; D000818:Animals; D017360:Arabidopsis / Q000235:genetics; D001482:Base Composition; D018899:CpG Islands; D018744:DNA, Plant / Q000235:genetics*; D004330:Drosophila / Q000235:genetics; D015870:Gene Expression; D017344:Genes, Insect; D017343:Genes, Plant; D018745:Genome, Plant; D001467:Hordeum / Q000235:genetics*; D006801:Humans; D007438:Introns; D008969:Molecular Sequence Data; D018626:Retroelements; D013045:Species Specificity; D003313:Zea mays / Q000235:genetics
D018744:DNA, Plant; D018626:Retroelements