Molecular cloning and chromosomal localization of a prophenoloxidase cDNA from the malaria vector Anopheles gambiae.
A cDNA clone for prophenoloxidase was isolated from the most important human malaria vector, Anopheles gambiae. The clone encoded a polypeptide of 79341 Da that contains the two copper binding domains common to all invertebrate prophenoloxidases and haemocyanins. Expression of the prophenoloxidase gene was detected throughout all life stages from egg to imago in two strains of A. gambiae; however, the strongest expression was observed in developing embryos in eggs. The prophenoloxidase gene was mapped to the inversion rich region of the right arm of chromosome-2 in region 13B.
Lee
W J
WJ
Medical Research Center, College of Medicine, Yonsei University, Seoul, South Korea.
Ahmed
A
A
della Torre
A
A
Kobayashi
A
A
Ashida
M
M
Brey
P T
PT
D000595:Amino Acid Sequence; D000818:Animals; D000852:Anopheles / Q000201:enzymology* / Q000235:genetics; D001483:Base Sequence; D004156:Catechol Oxidase / Q000235:genetics*; D002415:Cats; D002874:Chromosome Mapping; D003001:Cloning, Molecular; D018076:DNA, Complementary; D004792:Enzyme Precursors / Q000235:genetics*; D015870:Gene Expression; D017344:Genes, Insect; D006801:Humans; D019476:Insect Proteins / Q000235:genetics; D007303:Insect Vectors / Q000201:enzymology* / Q000235:genetics; D008288:Malaria / Q000635:transmission; D008969:Molecular Sequence Data; D012333:RNA, Messenger; D011817:Rabbits; D017386:Sequence Homology, Amino Acid
D018076:DNA, Complementary; D004792:Enzyme Precursors; D019476:Insect Proteins; D012333:RNA, Messenger; C038451:pro-phenoloxidase; D004156:Catechol Oxidase