The interaction between vitamin A and thiazolidinedione on bovine adipocyte differentiation in primary culture.
We studied the effects of a thiazolidinedione (T-174) and retinoids on adipocyte differentiation in beef cattle. Stromal-vascular (SV) cells containing preadipocytes were prepared from perirenal adipose tissue of 21-mo-old Japanese Black steers. After confluence, these cells were cultured in 25 microM T-174, which is a specific ligand for an adipogenesis stimulating nuclear receptor (i.e., gamma subtype of peroxisome proliferator-activated receptor [PPARgamma]), with 1 microM all-trans retinoic acid (RA) or .4 to 40 microg/100 mL of retinol for 10 to 14 d. The number of cells accumulating lipid droplets was counted as morphologically differentiated adipocytes, and the activity of glycerol-3-phosphate dehydrogenase (GPDH), a biochemical index for the differentiation, was determined. The number of lipid-laden cells and GPDH activity were increased by the addition of T-174. All-trans retinoic acid completely blocked the stimulative action of T-174. The addition of retinol also decreased the number of lipid-laden cells and GPDH activity in a dose-dependent manner. These results showed that the thiazolidinedione stimulated adipocyte differentiation and the retinoids blocked the adipogenesis induced by the thiazolidinedione in primary culture of bovine SV cells. Peroxisome proliferator-activated receptor gamma may play an important role in adipocyte differentiation, and retinoids may interfere with the action of PPARgamma in cattle.
Ohyama
M
M
Department of Animal Science, Faculty of Agriculture, Kyoto University, Japan.
Matsuda
K
K
Torii
S
S
Matsui
T
T
Yano
H
H
Kawada
T
T
Ishihara
T
T
D017667:Adipocytes / Q000166:cytology* / Q000187:drug effects / Q000201:enzymology; D000818:Animals; D015153:Blotting, Western; D002417:Cattle / Q000502:physiology*; D002454:Cell Differentiation / Q000187:drug effects / Q000502:physiology; D002478:Cells, Cultured; D004305:Dose-Response Relationship, Drug; D004347:Drug Interactions; D004730:Endothelium, Vascular / Q000166:cytology / Q000502:physiology; D005993:Glycerolphosphate Dehydrogenase / Q000032:analysis; D008024:Ligands; D008297:Male; D018160:Receptors, Cytoplasmic and Nuclear / Q000502:physiology; D017154:Stromal Cells / Q000166:cytology / Q000502:physiology; D013844:Thiazoles / Q000494:pharmacology*; D045162:Thiazolidinediones; D014157:Transcription Factors / Q000502:physiology; D014212:Tretinoin / Q000494:pharmacology; D014801:Vitamin A / Q000494:pharmacology*
D008024:Ligands; D018160:Receptors, Cytoplasmic and Nuclear; D013844:Thiazoles; D045162:Thiazolidinediones; D014157:Transcription Factors; D014801:Vitamin A; D014212:Tretinoin; C089946:2,4-thiazolidinedione; D005993:Glycerolphosphate Dehydrogenase