A monoclonal antibody to inclusion body disease of cranes virus enabling specific immunohistochemistry and competitive ELISA.
Inclusion body disease of cranes (IBDC) herpesvirus kills some infected cranes and persists in convalescent animals. To enable further study and rapid identification of carrier animals, we developed a monoclonal antibody (MAb) to IBDC virus and used it in immunohistochemistry and a competitive enzyme-linked immunosorbent assay (ELISA). We used conventional techniques to make murine MAbs directed against IBDC virus purified from infected duck embryo cells. Hybridomas reacting in an ELISA with IBDC virus but not uninfected duck embryo cells were characterized by radioimmunoprecipitation, in situ immunohistochemistry, and competitive ELISA with neutralizing and nonneutralizing crane sera. MAb 2C11 immunoprecipitated 59-, 61-, and 110-kD proteins from IBDC virus-infected but not uninfected cells and stained glutaraldehyde-fixed IBDC virus plaques but not surrounding uninfected duck embryo cells in vitro. Antibody 2C11 did not react with duck embryo cells infected with falcon herpesvirus, psittacine herpesvirus, infectious laryngotracheitis, pigeon herpesvirus, or duck plague virus. A competitive ELISA using antibody 2C11 identified most sera that were positive in the neutralization test. This antibody will be useful in further characterizing IBDC virus, its pathogenesis, and its natural history.
Letchworth
G J
GJ
Animal Health and Biomedical Sciences, University of Wisconsin-Madison 53706, USA.
Fishel
J R
JR
Hansen
W
W
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D000914:Antibodies, Viral / Q000276:immunology*; D000918:Antibody Specificity; D001667:Binding, Competitive; D001715:Bird Diseases / Q000276:immunology*; D001717:Birds; D002478:Cells, Cultured; D004372:Ducks / Q000196:embryology; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods / Q000662:veterinary; D006564:Herpesviridae / Q000276:immunology*; D006566:Herpesviridae Infections / Q000276:immunology / Q000662:veterinary*; D006825:Hybridomas; D007150:Immunohistochemistry / Q000379:methods; D051379:Mice; D011233:Precipitin Tests / Q000379:methods / Q000662:veterinary
D000911:Antibodies, Monoclonal; D000914:Antibodies, Viral