Fine mapping of bovine herpesvirus 1 (BHV-1) glycoprotein C neutralizing epitopes by type-specific monoclonal antibodies and synthetic peptides.
Bovine herpesvirus glycoprotein C (gC) functions as a major virus attachment protein. Here, two BHV-1 gC-specific epitopes that are specified by complement-dependent neutralizing MAbs are mapped. The BHV-1 gC-specific peptides and MAbs were used to specifically localize continuous epitopes by direct binding to the MAbs and by blocking the Mab reactivity (competitive ELISA) to authentic viral antigen. The results of competitive ELISA indicated that the complement-dependent neutralizing epitopes recognized by MAbs F2 and 24 were located between BHV-1 gC amino acids (aa) 47-69 and (aa) 109-119, respectively.
Chowdhury
S I
SI
Department of Diagnostic Medicine, Pathobiology College of Veterinary Medicine, Kansas State University, Manhattan 66506, USA. chowdh@vet.ksu.edu
D000818:Animals; D000911:Antibodies, Monoclonal; D000918:Antibody Specificity; D000956:Antigens, Viral; D002417:Cattle; D004797:Enzyme-Linked Immunosorbent Assay; D000939:Epitopes; D007242:Herpesvirus 1, Bovine / Q000302:isolation & purification; D009500:Neutralization Tests; D010446:Peptide Fragments / Q000138:chemical synthesis; D014764:Viral Proteins / Q000032:analysis / Q000737:chemistry* / Q000276:immunology*
D000911:Antibodies, Monoclonal; D000956:Antigens, Viral; D000939:Epitopes; D010446:Peptide Fragments; D014764:Viral Proteins; C046998:bovine herpesvirus type-1 glycoproteins