Measurement of platelet aggregation peptide inhibitors by ultrasonic interferometry.
Several peptide inhibitors of thrombin- or collagen-induced platelet aggregation and of the interaction between glycoprotein Ib and von Willebrand factor were studied by a new method--ultrasonic interferometry (Echo Cell). Inhibition of aggregate formation in a concentration-dependent manner was observed. The sensitivity of the method was 3 to 40 times higher than that of classical turbidimetry.
Chabance
B
B
CNRS URA 1188, Laboratoire des Protéines, Université de Paris V, Paris, France.
Perrotin
P
P
Guillet
R
R
Boynard
M
M
Migliore-Samour
D
D
Jollès
P
P
Fiat
A M
AM
D000328:Adult; D000818:Animals; D001426:Bacterial Proteins / Q000494:pharmacology; D002417:Cattle; D005260:Female; D006801:Humans; D007368:Interferometry / Q000379:methods; D008297:Male; D008853:Microscopy / Q000379:methods; D008875:Middle Aged; D009391:Nephelometry and Turbidimetry; D009842:Oligopeptides / Q000494:pharmacology; D055095:Optics and Photonics; D010455:Peptides / Q000494:pharmacology*; D010973:Platelet Adhesiveness / Q000187:drug effects; D010975:Platelet Aggregation Inhibitors / Q000494:pharmacology*; D014465:Ultrasonics
D001426:Bacterial Proteins; D009842:Oligopeptides; D010455:Peptides; D010975:Platelet Aggregation Inhibitors; C086512:glucose facilitator protein, Zymomonas; C046617:arginyl-glycyl-aspartyl-serine