title: An anaerobic, intrachamber incubator for growth of Methanosarcina spp. on methanol-containing solid media.
abstract: To simplify the incubation of Methanosarcina spp. on solid agar medium, a two-port, manual, rectangular air lock was modified to serve as an anaerobic incubator. In one operation, it is possible to incubate 153 petri plates, the equivalent of 11 standard anaerobic jars, with plating efficiencies identical to those of traditional protocols.
authors.affiliation: Department of Microbiology, University of Illinois, Urbana 61801, USA. metcalf@uiuc.edu
authors.forename: W W
authors.initials: WW
authors.lastname: Metcalf
authors.forename: J K
authors.initials: JK
authors.lastname: Zhang
authors.forename: R S
authors.initials: RS
authors.lastname: Wolfe
mesh_terms: D000693:Anaerobiosis; D000432:Methanol / Q000378:metabolism*; D017020:Methanosarcina / Q000254:growth & development*
chemical_list: D000432:Methanol