title: Protein-binding properties of the putative AP-1 and ATF sequences in the feline immunodeficiency virus long terminal repeat.
abstract: Electrophoresis-mobility-shift assays with nuclear extracts from a feline renal cell line and a T-lymphoblastoid cell line revealed that the AP-1 and ATF sites of feline immunodeficiency virus (FIV) TM2 strain had similar protein-binding properties to those of FIV Petaluma strain and consensus sequences of AP-1 and ATF sites, and that nuclear factors binding to these sites differed between the two cell lines. Cross-competition and gel-supershift assays demonstrated that the AP-1 and ATF sites had similar protein-binding properties. The effects of internal deletions of AP-1 and/or ATF sites on the basal promoter activity were also examined. Although deletion of either site moderately reduced activity, a mutant deleted in both sites had dramatically reduced activity. Therefore, we suggest that these two sites co-operatively regulate transcriptional activity of the promoter.
authors.affiliation: Department of Veterinary Microbiology, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Japan.
authors.forename: Y
authors.initials: Y
authors.lastname: Ikeda
authors.forename: Y
authors.initials: Y
authors.lastname: Inoshima
authors.forename: Y
authors.initials: Y
authors.lastname: Kawaguchi
authors.forename: K
authors.initials: K
authors.lastname: Maeda
authors.forename: M
authors.initials: M
authors.lastname: Kohmoto
authors.forename: C
authors.initials: C
authors.lastname: Kai
authors.forename: T
authors.initials: T
authors.lastname: Miyazawa
authors.forename: T
authors.initials: T
authors.lastname: Mikami
mesh_terms: D051696:Activating Transcription Factors; D000818:Animals; D001483:Base Sequence; D001798:Blood Proteins / Q000235:genetics / Q000378:metabolism*; D002415:Cats; D002460:Cell Line; D004279:DNA, Viral; D016089:Immunodeficiency Virus, Feline / Q000235:genetics*; D008969:Molecular Sequence Data; D009687:Nuclear Proteins / Q000378:metabolism*; D009841:Oligonucleotides / Q000378:metabolism; D011485:Protein Binding; D012091:Repetitive Sequences, Nucleic Acid; D018808:Transcription Factor AP-1 / Q000235:genetics / Q000378:metabolism*; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism*
chemical_list: D051696:Activating Transcription Factors; D001798:Blood Proteins; D004279:DNA, Viral; D009687:Nuclear Proteins; D009841:Oligonucleotides; D018808:Transcription Factor AP-1; D014157:Transcription Factors