Regulation of B lymphocyte development by the truncated immunoglobulin heavy chain protein Dmu.
The development of B lymphocytes from progenitor cells is dependent on the expression of a pre-B cell-specific receptor made up by a mu heavy chain associated with the surrogate light chains, immunoglobulin (Ig)alpha, and Igbeta. A variant pre-B cell receptor can be formed in which the mu heavy chain is exchanged for a truncated mu chain denoted Dmu. To investigate the role of this receptor in the development of B cells, we have generated transgenic mice that express the Dmu protein in cells of the B lineage. Analysis of these mice reveal that Dmu expression leads to a partial block in B cell development at the early pre-B cell stage, probably by inhibiting VH to DHJH rearrangement. Furthermore, we provide evidence that Dmu induces VL to JL rearrangements.
Tornberg
U C
UC
Department of Cell and Molecular Biology, Umeâ University, S-901 87 Umeâ, Sweden.
Bergqvist
I
I
Haury
M
M
Holmberg
D
D
D000818:Animals; D000831:Animals, Newborn; D001402:B-Lymphocytes / Q000166:cytology*; D002454:Cell Differentiation; D018507:Gene Expression Regulation, Developmental; D015326:Gene Rearrangement, B-Lymphocyte, Heavy Chain; D015328:Gene Rearrangement, B-Lymphocyte, Light Chain; D007148:Immunoglobulin mu-Chains / Q000276:immunology*; D008099:Liver / Q000166:cytology; D051379:Mice; D008822:Mice, Transgenic; D011401:Promoter Regions, Genetic; D011947:Receptors, Antigen, B-Cell / Q000502:physiology*; D013154:Spleen / Q000166:cytology
D007148:Immunoglobulin mu-Chains; D011947:Receptors, Antigen, B-Cell