[Evaluation of Novapath assay for direct detection in patient stool of Vero toxin and Vero toxin-producing Escherichia coli].
We evaluated two kits which detect either Verotoxin (Novapath EHEC) or EHEC O157 (Novapath O157) directly from stool specimens and compared with the result of PCR. The tests can be completed in 3 hrs. All fecal specimens from 18 healthy volunteers showed negative results with both kits and PCR. Specimens from patients of EHEC O157 outbreak in Okayama Prefecture and asymptomatic families of patients of outbreak in Sakai City of Japan were also analyzed. Fecal specimens from which EHEC O157 was isolated showed positive results with both kits and PCR. Some specimens which were culture negative showed positive results with either Novapath EHEC or Novapath O157 or both. The sensitivity of Novapath O157 was lower than PCR to detect O157 organisms but took shorter time to get result. Novapath EHEC showed very high sensitivity in detecting Vero toxin. These results suggest that Novapath EHEC especially can be used clinically for rapid diagnosis.
Yoh
M
M
Research Center for Emerging Infectious Diseases, Osaka University.
Iida
T
T
Okada
Y
Y
Honda
T
T
D001427:Bacterial Toxins / Q000032:analysis*; D004927:Escherichia coli Infections / Q000382:microbiology*; D019453:Escherichia coli O157 / Q000302:isolation & purification* / Q000378:metabolism; D005069:Evaluation Studies as Topic; D005243:Feces / Q000737:chemistry*; D006801:Humans; D011933:Reagent Kits, Diagnostic / Q000592:standards*; D022622:Shiga Toxin 1
D001427:Bacterial Toxins; D011933:Reagent Kits, Diagnostic; D022622:Shiga Toxin 1