Evidence for a local action of melatonin on the rat prostate.
OBJECTIVE
Melatonin, secreted by the pineal gland at night, inhibits pubertal development in rats and possibly humans. We have recently found functional specific binding sites for 125I-labeled melatonin (125I-melatonin) in human benign prostate tissue, localized in the microsomal fraction of the glandular epithelium. The aim of the present study was to set up an animal (rodent) model for the growth inhibitory effects of melatonin on the prostate.


METHODS
Putative melatonin in the rat ventral prostate were explored by means of autoradiography and receptor binding assays and the ability of melatonin to inhibit stimulated prostate growth was tested in vivo.


RESULTS
In vitro autoradiography and equilibrium binding experiments demonstrated specific binding sites for 125I-labeled melatonin (125I-melatonin) associated with the microsomal fraction of the rat ventral prostate cells (apparent dissociation constant 0.9 nM). 125I-melatonin binding was inhibited by 2-iodomelatonin > 6-hydroxy-melatonin > melatonin = N-(2,4 dinitrophenyl)-5-methoxytryptamine whereas similar concentrations of serotonin, 5-methoxytryptamine, and tryptamine were less potent. The guanine nucleotide analogs, guanosine 5'-0-[3-thiotriphosphate] and guanosine 5'-0-[2-thio-diphosphate], inhibited specific 125I-melatonin binding whereas 5'-guanylyl imido-diphosphate was less potent. Daily injections of testosterone to castrated rats induced regrowth of the prostate and increased the weight of the seminal vesicles. Administration of melatonin to the rats through drinking water prevented the testosterone-mediated regrowth of the prostate but had no effect on the seminal vesicles' weight.


CONCLUSIONS
The results demonstrate putative melatonin receptors in the rat prostate and suggest a direct suppression by melatonin of testosterone-dependent prostate growth.
Gilad
E
E
Department of Neurobiochemistry, George S. Wise Faculty of Life Sciences, Tel-Aviv University, Neurim Pharmaceuticals, Israel.
Laudon
M
M
Matzkin
H
H
Zisapel
N
N
D000818:Animals; D001345:Autoradiography; D001665:Binding Sites; D007457:Iodine Radioisotopes; D008297:Male; D008550:Melatonin / Q000502:physiology*; D008861:Microsomes; D011467:Prostate / Q000502:physiology*; D051381:Rats; D011919:Rats, Inbred Strains
D007457:Iodine Radioisotopes; D008550:Melatonin