Ultrasensitive bienzyme sensor for adrenaline.
A biosensor consisting of an analyte-recycling two-enzyme system using laccase (Coriolus hirsutus) and PQQ-dependent glucose dehydrogenase in combination with the electrochemical detection of oxygen depletion at a platinum electrode was used for adrenaline determination in the nano- and subnanomolar concentration range. Measurements were performed in a flow cell providing excellent baseline stability and fast recovery of the sensor. Improved design of the polymer matrix resulted in a lower detection limit of 200 pmol/l for adrenaline. The sensor has successfully been applied to the analysis of adrenaline in effluate of isolated rabbit hearts.
Szeponik
J
J
BST Bio Sensor Technologie GmbH, Berlin, Germany.
Möller
B
B
Pfeiffer
D
D
Lisdat
F
F
Wollenberger
U
U
Makower
A
A
Scheller
F W
FW
D000818:Animals; D015374:Biosensing Techniques; D004563:Electrochemistry; D004837:Epinephrine / Q000032:analysis*; D042843:Glucose 1-Dehydrogenase; D005948:Glucose Dehydrogenases; D066298:In Vitro Techniques; D042845:Laccase; D008832:Microchemistry; D009206:Myocardium / Q000737:chemistry*; D010088:Oxidoreductases; D010100:Oxygen / Q000032:analysis*; D011108:Polymers; D011817:Rabbits; D012680:Sensitivity and Specificity
D011108:Polymers; D010088:Oxidoreductases; D005948:Glucose Dehydrogenases; D042843:Glucose 1-Dehydrogenase; D042845:Laccase; D010100:Oxygen; D004837:Epinephrine