Cloning of a putative human neurotransmitter receptor expressed in skeletal muscle and brain.
With the use of the degenerated nucleotides that contain the conserved sequence of G protein-coupled receptor, we have identified a 648-bp clone (HDGRC02) from human genomic DNA with significant sequence homology to human neurotransmitter receptors. HDGRC02 was then used as a probe for the screening of full length gene. From human Lambda DASH II genomic library, a 1.6 Kb clone encoded a full length gene was isolated and named putative neurotransmitter receptor (PNR). PNR has a single open reading frame which predicts a 38.3 KD protein of 338 amino acids with seven transmembrane domain topography. The amino acid sequence of PNR exhibits considerable homology to the rat 5-HR1D receptor with 35% amino acid identity and 56% amino acid similarity. PNR also shows significant sequence homology to the 5-HT1D receptor from Japanese puffer fish fugu, to the 5-HT4L receptor from mouse, to the alpha-2 adrenergic receptor and to the D2 dopamine receptor. Northern blot analysis indicates that PNR is expressed in skeletal muscle and selected areas of the brain. A chromosome mapping study located the PNR gene with human chromosome band of 6q23. The findings in the present study demonstrate that PNR is a putative neurotransmitter receptor.
Zeng
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Human Genome Sciences Incorporated, Rockville, Maryland 20850, USA.
Fan
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Rand
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Kyaw
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Su
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Madike
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Carter
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Li
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D000595:Amino Acid Sequence; D001483:Base Sequence; D015152:Blotting, Northern; D001923:Brain Chemistry; D002874:Chromosome Mapping; D002896:Chromosomes, Human, Pair 6; D003001:Cloning, Molecular; D006801:Humans; D017403:In Situ Hybridization; D008969:Molecular Sequence Data; D018482:Muscle, Skeletal / Q000737:chemistry*; D012333:RNA, Messenger / Q000032:analysis; D017981:Receptors, Neurotransmitter / Q000737:chemistry*; D011985:Receptors, Serotonin / Q000737:chemistry; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA
D012333:RNA, Messenger; D017981:Receptors, Neurotransmitter; D011985:Receptors, Serotonin