title: Isolation and sequence analysis of a human cDNA clone (XPNPEPL) homologous to X-prolyl aminopeptidase (aminopeptidase P).
abstract: A novel human cDNA (XPNPEPL) encoding a protein of 623 amino acids exhibiting 44% sequence identity and 62% sequence similarity to pig kidney X-prolyl aminopeptidase (aminopeptidase P; EC 3.4.11.9) was obtained by reverse transcription/polymerase chain reaction of phytohemagglutinin-stimulated lymphocyte mRNA. Conserved sequences were found with the prokaryotic X-prolyl aminopeptidase encoding gene (pepP). The human gene translation product exhibits a high sequence homology to the Schizosaccharomyces pombe chromosome I hypothetical protein C22G7.01c and to the S. cerevisiae ORF y11029w. Northern blot analysis indicates an ubiquitous expression of the human XPNPEPL sequence.
authors.affiliation: Department of Clinical Biochemistry, University of Antwerp, Wilrijk, Belgium. gvhoof@uia.ua.ac.be
authors.forename: G
authors.initials: G
authors.lastname: Vanhoof
authors.forename: F
authors.initials: F
authors.lastname: Goossens
authors.forename: M A
authors.initials: MA
authors.lastname: Juliano
authors.forename: L
authors.initials: L
authors.lastname: Juliano
authors.forename: D
authors.initials: D
authors.lastname: Hendriks
authors.forename: K
authors.initials: K
authors.lastname: Schatteman
authors.forename: A H
authors.initials: AH
authors.lastname: Lin
authors.forename: S
authors.initials: S
authors.lastname: Scharpé
mesh_terms: D000626:Aminopeptidases / Q000235:genetics*; D000818:Animals; D001483:Base Sequence; D015152:Blotting, Northern; D003001:Cloning, Molecular; D017124:Conserved Sequence; D017931:DNA Primers; D018076:DNA, Complementary; D006801:Humans; D008969:Molecular Sequence Data; D012333:RNA, Messenger / Q000032:analysis; D017386:Sequence Homology, Amino Acid; D013552:Swine
chemical_list: D017931:DNA Primers; D018076:DNA, Complementary; D012333:RNA, Messenger; D000626:Aminopeptidases; C022750:X-Pro aminopeptidase