Effects of photoisomers of cyclodiene insecticides on liver and microsomal cytochrome P450 in rats.
Threshold dosages of the photoisomers of cyclodiene insecticides, namely photochlordane, photodieldrin, and photoheptachlor, for the induction of hepatic microsomal cytochrome P450 (P450) and liver hypertrophy in male rats were at least one-quarter of those reported for corresponding parent cyclodienes. Maximum increase in total P450 concentration (30%) and demethylases activities (100%) was always respectively one-third or one-tenth of that reported for parent cyclodienes. The P450 isozymic form induced by photoheptachlor resembled that induced by pentobarbital (P4502B1) in its substrate specificity, spectral characteristics, and electrophoretic mobility. The induction of P450 was initially followed by hepatic hypertrophy. However, higher dosages of photoisomers caused wasting and lowered both the liver weight and the activity of aniline hydroxylase while those of mirex and endrin, which also caused wasting and lowered aniline hydroxylase activity, continued causing further hepatic hypertrophy.
Khan
M A
MA
Department of Biological Sciences, University of Illinois at Chicago, 60607-7060, USA.
Jovanovich
L V
LV
Martin
L T
LT
Qadri
S Y
SY
Podowski
A A
AA
D000815:Aniline Hydroxylase / Q000378:metabolism; D000818:Animals; D001835:Body Weight / Q000187:drug effects; D002706:Chlordan / Q000031:analogs & derivatives* / Q000633:toxicity; D003577:Cytochrome P-450 Enzyme System / Q000096:biosynthesis*; D004026:Dieldrin / Q000031:analogs & derivatives* / Q000633:toxicity; D004790:Enzyme Induction / Q000187:drug effects; D006533:Heptachlor / Q000031:analogs & derivatives* / Q000633:toxicity; D006984:Hypertrophy / Q000139:chemically induced; D007306:Insecticides / Q000633:toxicity*; D007527:Isoenzymes / Q000096:biosynthesis*; D008099:Liver / Q000187:drug effects / Q000201:enzymology / Q000473:pathology; D008297:Male; D008862:Microsomes, Liver / Q000187:drug effects* / Q000201:enzymology; D008917:Mirex / Q000633:toxicity*; D009929:Organ Size / Q000187:drug effects; D010424:Pentobarbital; D051381:Rats; D017207:Rats, Sprague-Dawley; D013379:Substrate Specificity
D007306:Insecticides; D007527:Isoenzymes; D002706:Chlordan; C008892:photodieldrin; C018638:photoheptachlor; C025640:photochlordane; D006533:Heptachlor; D003577:Cytochrome P-450 Enzyme System; D000815:Aniline Hydroxylase; D004026:Dieldrin; D010424:Pentobarbital; D008917:Mirex