Induction of macrophage-inflammatory protein 1alpha (MIP-1alpha) by interferon-alpha.
The biological functions of macrophage-inflammatory protein 1alpha (MIP-1alpha) include the regulation of inflammatory processes and the inhibition of early hematopoiesis. We investigated the regulation of MIP-1alpha in various cell types in response to IFN-alpha and IL-4 by mRNA analysis and examination of MIP-1alpha protein levels in culture supernatants. In peripheral blood mononuclear cells (PBMNCs) and purified monocytes, IFN-alpha induced MIP-1alpha mRNA expression and stimulated MIP-1alpha secretion in LPS-activated monocytes to almost twice the value observed with LPS alone, whereas IL-4 caused a reduction by 80-95%. The stimulatory effect of IFN-alpha on MIP-1alpha production appeared to be dominant over its inhibition by IL-4. This effect was most pronounced after prolonged incubation periods. In fibroblasts obtained from human foreskin, IFN-alpha in combination with IL-1 stimulated MIP-1alpha secretion to more than three times the level obtained with IL-1. Stimulation of MIP-1alpha expression by IFN-alpha may indirectly influence inflammatory processes and the regulation of early hematopoiesis.
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IIIrd Department of Medicine, The Johannes Gutenberg University School of Medicine, Mainz, Germany.
Aman
M J
MJ
Tretter
T
T
Huber
C
C
Peschel
C
C
D002478:Cells, Cultured; D054405:Chemokine CCL3; D054407:Chemokine CCL4; D005347:Fibroblasts / Q000166:cytology / Q000187:drug effects / Q000276:immunology; D005786:Gene Expression Regulation / Q000187:drug effects; D006801:Humans; D007231:Infant, Newborn; D000077190:Interferon alpha-2; D016898:Interferon-alpha / Q000494:pharmacology*; D007375:Interleukin-1 / Q000494:pharmacology*; D007700:Kinetics; D019402:Macrophage Inflammatory Proteins / Q000096:biosynthesis*; D008297:Male; D009000:Monocytes / Q000166:cytology / Q000187:drug effects / Q000276:immunology*; D012333:RNA, Messenger / Q000096:biosynthesis; D011994:Recombinant Proteins / Q000494:pharmacology; D012867:Skin / Q000166:cytology / Q000187:drug effects / Q000276:immunology; D014158:Transcription, Genetic / Q000187:drug effects*
D054405:Chemokine CCL3; D054407:Chemokine CCL4; D000077190:Interferon alpha-2; D016898:Interferon-alpha; D007375:Interleukin-1; D019402:Macrophage Inflammatory Proteins; D012333:RNA, Messenger; D011994:Recombinant Proteins