Mixed immunosorbent for selective on-line trace enrichment and liquid chromatography of phenylurea herbicides in environmental waters.
An immunosorbent containing antiisoproturon and antichlortoluron antibodies immobilised on aldehyde-activated silica was employed for the on-line preconcentration and liquid chromatography-diode array detection of several phenylureas. The efficiency of the coupling of the immunosorbent to the liquid chromatographic system and the characteristics of the immunosorbent were evaluated. The on-line system allowed the selective trace enrichment of chlortoluron, isoproturon, metobromuron, linuron and chlorbromuron at the 0.05-0.5 microgram l-1 level in ground and river waters and provided detection limits in the range 0.01-0.03 microgram l-1 by percolating only 10 ml of water sample. The proposed method was validated by analysing freeze-dried tap water samples with a high content in pesticides of different chemical functionalities. The results obtained were compared with the mean value obtained in an interlaboratory exercise.
Martin-Esteban
A
A
Departamento de Química Analítica, Facultad de Ciencias Químicas, Universidad Complutense de Madrid, Spain.
Fernández
P
P
Stevenson
D
D
Cámara
C
C
D002853:Chromatography, Liquid / Q000379:methods; D006540:Herbicides / Q000032:analysis*; D007164:Immunosorbents; D010671:Phenylurea Compounds / Q000032:analysis*; D014874:Water Pollutants, Chemical / Q000032:analysis*
D006540:Herbicides; D007164:Immunosorbents; D010671:Phenylurea Compounds; D014874:Water Pollutants, Chemical