Co-mutagenicity of glyco- and tauro-deoxycholic acids in the Ames test.
Mutagenicity and co-mutagenicity of glyco- and tauro-deoxycholic acids (GDCA and TDCA), which are abundant in human bile, were examined by the Ames test. The two chemicals were not mutagenic for themselves to Salmonella typhimurium TA98 and TA100, with and without S9 mix. They enhanced, however, the mutagenic activities of the pro-mutagens, 2-aminoanthracene (2AA) and benzo[a]pyrene (BaP), for both TA98 and TA100 with S9 mix. They were more co-mutagenic for the pro-mutagens on TA98 than on TA100. On TA98, the mutagenic activities of 2AA with GDCA (5 mumol/plate) and with TDCA (5 mumol/plate) were 9.7-fold and 11.8-fold as high as that of the corresponding control (2AA only), respectively. BaP with GDCA (2.5 mumol/plate) and with TDCA (2.5 mumol/plate) showed 2.8-fold and 3.0-fold increases over the corresponding control level (BaP only), respectively. It is hence concluded that GDCA and TDCA may enhance the activity of some mutagens existing in bile.
Shibuya
N
N
Biochemical Laboratory, Niigata College of Medical Technology, Japan.
Nakadaira
H
H
Ohta
T
T
Nakamura
K
K
Hori
Y
Y
Yamamoto
M
M
Saitoh
Y
Y
Ogoshi
K
K
D000818:Animals; D000873:Anthracenes / Q000633:toxicity; D001564:Benzo(a)pyrene / Q000633:toxicity; D003840:Deoxycholic Acid / Q000633:toxicity; D004305:Dose-Response Relationship, Drug; D006002:Glycodeoxycholic Acid / Q000633:toxicity*; D009152:Mutagenicity Tests; D009153:Mutagens / Q000633:toxicity*; D051381:Rats; D017207:Rats, Sprague-Dawley; D013657:Taurodeoxycholic Acid / Q000633:toxicity*
D000873:Anthracenes; D009153:Mutagens; D003840:Deoxycholic Acid; D001564:Benzo(a)pyrene; D006002:Glycodeoxycholic Acid; D013657:Taurodeoxycholic Acid; C006593:2-anthramine