Polymerase chain reaction-based identification of a novel serpin from human dendritic cells.
A subtraction library of CD40-stimulated human tonsil dendritic cells has been constructed using a polymerase chain reaction approach adapted for low numbers of cells. From this library we identified a cDNA for a serine protease inhibitor, a serpin, which is absent from monocytes, B cells and T cells but expressed in CD40-activated monocyte- and progenitor cell-generated dendritic cells. In addition, the serpin is expressed in a lung fibroblast cell line and keratinocytes. Its mRNA is detected only in tonsil and thymus. The serpin described here reportedly functions as a megakaryocyte maturation factor in the presence of interleukin (IL)-3 and IL-11. This suggests that dendritic cells may promote the immune response by protecting IL-3 and IL-11 or other essential proteins from degradation.
Mueller
C G
CG
Schering-Plough, Laboratory for Immunological Research, Dardilly, France.
Ho
S
S
Massacrier
C
C
Lebecque
S
S
Liu
Y J
YJ
D000595:Amino Acid Sequence; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics; D003713:Dendritic Cells / Q000276:immunology*; D006801:Humans; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D016415:Sequence Alignment; D017421:Sequence Analysis; D015843:Serpins / Q000235:genetics* / Q000276:immunology*
D018076:DNA, Complementary; D015843:Serpins