Induction of ornithine decarboxylase mRNA in transient focal cerebral ischemia in the rat.
We used in situ hybridization to localize the long-term changes in ornithine decarboxylase (ODC) expression after a 90 min occlusion of the middle cerebral artery (MCAO) in the rat. The ODC mRNA was induced in the ipsilateral dentate gyrus (DG) and throughout the ischemic cortex at 12 h and still at 3 days after reperfusion. The induction was blocked by an N-methyl-D-aspartate (NMDA) receptor antagonist suggesting that ODC induction is NMDA receptor-mediated. The long-lasting up-regulation detected in regions where no cellular damage usually occurs, favors the hypothesis that ODC expression does not contribute to neuronal death after stroke.
Keinänen
R
R
A.I. Virtanen Institute, University of Kuopio, Finland. riitta.keinanen@uku.fi
Miettinen
S
S
Yrjänheikki
J
J
Koistinaho
J
J
D000818:Animals; D015152:Blotting, Northern; D016291:Dizocilpine Maleate / Q000494:pharmacology; D005786:Gene Expression Regulation / Q000187:drug effects; D017403:In Situ Hybridization; D002546:Ischemic Attack, Transient / Q000201:enzymology*; D008297:Male; D018696:Neuroprotective Agents / Q000494:pharmacology; D009955:Ornithine Decarboxylase / Q000378:metabolism*; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D016194:Receptors, N-Methyl-D-Aspartate / Q000037:antagonists & inhibitors; D013997:Time Factors
D018696:Neuroprotective Agents; D012333:RNA, Messenger; D016194:Receptors, N-Methyl-D-Aspartate; D016291:Dizocilpine Maleate; D009955:Ornithine Decarboxylase