title: Discontinuous Percoll gradient preparation for donor insemination: determinants for success.
abstract: The use of cryopreserved donor spermatozoa for insemination has become necessary to decrease the risk from sexually transmitted infectious diseases. Lower fecundity rates have been reported with this practice. Efforts have been applied to increase success, including identification of those sperm characteristics which correlate with increased fecundity. Data from in-vitro fertilization have revealed sperm morphology, motility and zona pellucida binding as important sperm parameters. Discontinuous Percoll gradient preparation yields a high concentration of motile spermatozoa. Using this preparation for thawed donor spermatozoa, we have identified post-preparation motility and progression as factors associated with increased fecundity. Consideration should be given to screening sperm donors with a freeze-thaw Percoll gradient preparation prior to acceptance into a donor bank.
authors.affiliation: Department of Obstetrics and Gynecology, Thomas Jefferson University School of Medicine, Pennsylvania Hospital, Philadelphia, USA.
authors.forename: M P
authors.initials: MP
authors.lastname: Kelly
authors.forename: S L
authors.initials: SL
authors.lastname: Corson
authors.forename: B
authors.initials: B
authors.lastname: Gocial
authors.forename: F R
authors.initials: FR
authors.lastname: Batzer
authors.forename: J N
authors.initials: JN
authors.lastname: Gutmann
mesh_terms: D000328:Adult; D002499:Centrifugation, Density Gradient; D006063:Chorionic Gonadotropin / Q000008:administration & dosage; D015925:Cryopreservation; D005260:Female; D005306:Fertilization; D006801:Humans; D007316:Insemination, Artificial, Heterologous; D007986:Luteinizing Hormone / Q000652:urine; D008297:Male; D011247:Pregnancy; D011256:Pregnancy Outcome; D012662:Semen Preservation; D012749:Sexually Transmitted Diseases / Q000517:prevention & control; D013081:Sperm Motility
chemical_list: D006063:Chorionic Gonadotropin; D007986:Luteinizing Hormone