title: Vacuolar-type H+-ATPase regulates cytoplasmic pH in Toxoplasma gondii tachyzoites.
abstract: Cytoplasmic pH (pHi) regulation was studied in Toxoplasma gondii tachyzoites by using the fluorescent dye 2',7'-bis-(2-carboxyethyl)-5(6)-carboxyfluorescein. Their mean baseline pHi (7.07+/-0.06; n=5) was not significantly affected in the absence of extracellular Na+, K+ or HCO3(-) but was significantly decreased in a dose-dependent manner by low concentrations of N, N'-dicyclohexylcarbodi-imide (DCCD), N-ethylmaleimide (NEM) or bafilomycin A1. Bafilomycin A1 also inhibited the recovery of tachyzoite pHi after an acid load with sodium propionate. Similar concentrations of DCCD, NEM and bafilomycin A1 produced depolarization of the plasma membrane potential as measured with bis-(1,3-diethylthiobarbituric)trimethineoxonol (bisoxonol), and DCCD prevented the hyperpolarization that accompanies acid extrusion after the addition of propionate, in agreement with the electrogenic nature of this pump. Confocal laser scanning microscopy indicated that, in addition to being located in cytoplasmic vacuoles, the vacuolar (V)-H+-ATPase of T. gondii tachyzoites is also located in the plasma membrane. Surface localization of the V-H+-ATPase was confirmed by experiments using biotinylation of cell surface proteins and immunoprecipitation with antibodies against V-H+-ATPases. Taken together, the results are consistent with the presence of a functional V-H+-ATPase in the plasma membrane of these intracellular parasites and with an important role of this enzyme in the regulation of pHi homoeostasis in these cells.
authors.affiliation: Laboratory of Molecular Parasitology, Department of Pathobiology, College of Veterinary Medicine, University of Illinois at Urbana-Champaign, 2001 S. Lincoln Avenue, Urbana, IL 61802, USA.
authors.forename: S N
authors.initials: SN
authors.lastname: Moreno
authors.forename: L
authors.initials: L
authors.lastname: Zhong
authors.forename: H G
authors.initials: HG
authors.lastname: Lu
authors.forename: W D
authors.initials: WD
authors.lastname: Souza
authors.forename: M
authors.initials: M
authors.lastname: Benchimol
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology; D019921:Biotinylation; D002417:Cattle; D002712:Chlorides / Q000494:pharmacology; D003593:Cytoplasm / Q000502:physiology*; D004024:Dicyclohexylcarbodiimide / Q000494:pharmacology; D004791:Enzyme Inhibitors / Q000494:pharmacology; D005033:Ethylmaleimide / Q000494:pharmacology; D019084:Fluorescent Antibody Technique, Indirect; D006863:Hydrogen-Ion Concentration; D018942:Macrolides; D008564:Membrane Potentials; D011188:Potassium / Q000494:pharmacology; D006180:Proton-Translocating ATPases / Q000037:antagonists & inhibitors / Q000378:metabolism*; D012964:Sodium / Q000494:pharmacology; D014122:Toxoplasma / Q000201:enzymology* / Q000254:growth & development; D025262:Vacuolar Proton-Translocating ATPases
chemical_list: D000900:Anti-Bacterial Agents; D002712:Chlorides; D004791:Enzyme Inhibitors; D018942:Macrolides; D004024:Dicyclohexylcarbodiimide; C040929:bafilomycin A1; D012964:Sodium; D025262:Vacuolar Proton-Translocating ATPases; D006180:Proton-Translocating ATPases; D005033:Ethylmaleimide; D011188:Potassium