Differential effects of cholesterol and oxidised-cholesterol in egg lecithin bilayers.
Low frequency impedance measurements of pure egg lecithin (phosphatidylcholine) bilayers have revealed the presence of four layers which can be attributed to the acyl chain, carbonyl, glycerol bridge and phosphatidylcholine regions of the lecithin molecule. Measurements on bilayers formed in the presence of unoxidised-cholesterol revealed that cholesterol molecules were located in the hydrocarbon region of the bilayer with its hydroxyl groups aligned with the carbonyl region of the lecithin molecules. Measurements of oxidised-cholesterol lecithin bilayers revealed that these molecules protruded less into the hydrocarbon region and their polar hydroxyl group aligned with the glycerol bridge region of the lecithin molecule.
Karolis
C
C
UNESCO Centre for Membrane Science and Technology and Department of Biophysics, School of Physics, University of New South Wales, Sydney, Australia.
Coster
H G
HG
Chilcott
T C
TC
Barrow
K D
KD
D002784:Cholesterol / Q000737:chemistry*; D017097:Electric Impedance; D008051:Lipid Bilayers / Q000737:chemistry*; D010063:Ovum; D010084:Oxidation-Reduction; D010713:Phosphatidylcholines / Q000737:chemistry*
D008051:Lipid Bilayers; D010713:Phosphatidylcholines; D002784:Cholesterol