title: Evaluation of the 5' spliced form of human cathepsin B mRNA in colorectal mucosa and tumors.
abstract: To evaluate the 5' spliced form of human cathepsin B mRNA in colorectal mucosa and tumors, we have determined the ratio of the spliced form for the exon 2/the complete form of cathepsin B mRNAs obtained by RT-PCR. Such ratio is significantly higher in colorectal tumors than in colorectal mucosa (p < 0.05, Kruskal-Wallis test) or in skeletal muscle (p < 0.05). Moreover, 2-fold more complete form than the spliced mRNA was found in the tumors than in the colorectal mucosa. Our data indicate that the alternative splicing of human cathepsin B mRNA in the 5'UTR may be considered as an indicator of the cellular transformation, in colorectal cancer.
authors.affiliation: Laboratoire d'Oncologie Moléculaire, INSERM CRI 9402, Centre Jean Perrin, 58 rue Montalembert, B.P. 392, 63011 Clermont-Ferrand Cedex 1, France.
authors.forename: C
authors.initials: C
authors.lastname: Hizel
authors.forename: M
authors.initials: M
authors.lastname: Ferrara
authors.forename: H
authors.initials: H
authors.lastname: Cure
authors.forename: D
authors.initials: D
authors.lastname: Pezet
authors.forename: P
authors.initials: P
authors.lastname: Dechelotte
authors.forename: J
authors.initials: J
authors.lastname: Chipponi
authors.forename: P
authors.initials: P
authors.lastname: Rio
authors.forename: Y J
authors.initials: YJ
authors.lastname: Bignon
authors.forename: D
authors.initials: D
authors.lastname: Bernard-Gallon
mesh_terms: D017398:Alternative Splicing; D002401:Cathepsin B / Q000096:biosynthesis* / Q000235:genetics; D003106:Colon / Q000201:enzymology; D015179:Colorectal Neoplasms / Q000201:enzymology* / Q000473:pathology; D005091:Exons; D006801:Humans; D007413:Intestinal Mucosa / Q000201:enzymology* / Q000473:pathology; D007700:Kinetics; D018482:Muscle, Skeletal / Q000201:enzymology; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000096:biosynthesis*; D012007:Rectum / Q000201:enzymology; D014407:Tumor Cells, Cultured
chemical_list: D012333:RNA, Messenger; D002401:Cathepsin B