title: Expression and imprinting of the insulin-like growth factor II gene in neonatal mouse cerebellum.
abstract: Insulin-like growth factor II (IGF-II) plays significant roles in the growth and development of mammals through the regulation of mitogenesis and cell survival. Previously, IGF-II mRNA transcripts within the CNS were detected in the choroid plexus and leptomeninges (DeChiara et al., 1991). The objective of this study was to determine the expression pattern of IGF-II mRNA in different cell types of the cerebellum during development. We report here that the IGF-II gene is transcribed in granule and glial cells within the cerebellar parenchyma at various times during the early postnatal period in mice. IGF-II gene expression is further regulated by parent-specific imprinting such that only the paternal IGF-II allele is expressed in granule cells. In contrast, choroid plexus and leptomeninges express IGF-II mRNAs biallelically, indicating that cell type-specific regulation of genomic imprinting occurs within the mammalian CNS.
authors.affiliation: Department of Neurology, Children's Hospital and Harvard Medical School, Boston, Massachusetts, USA.
authors.forename: S W
authors.initials: SW
authors.lastname: Hetts
authors.forename: K M
authors.initials: KM
authors.lastname: Rosen
authors.forename: P
authors.initials: P
authors.lastname: Dikkes
authors.forename: L
authors.initials: L
authors.lastname: Villa-Komaroff
authors.forename: R L
authors.initials: RL
authors.lastname: Mozell
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D002478:Cells, Cultured; D002531:Cerebellum / Q000166:cytology / Q000378:metabolism*; D002831:Choroid Plexus / Q000378:metabolism; D005786:Gene Expression Regulation / Q000502:physiology*; D018392:Genomic Imprinting; D007335:Insulin-Like Growth Factor II / Q000235:genetics*; D051379:Mice; D008810:Mice, Inbred C57BL; D009474:Neurons / Q000378:metabolism; D016133:Polymerase Chain Reaction / Q000379:methods; D012333:RNA, Messenger / Q000032:analysis; D014158:Transcription, Genetic
chemical_list: D012333:RNA, Messenger; D007335:Insulin-Like Growth Factor II