Enhancement of CDDP cytotoxicity by caffeine is characterized by apoptotic cell death.
Through the use of STKM-1 human stomach cancer cells, we investigated the enhancement of the anti-tumor effect and the apoptosis induction of the CDDP and caffeine combination. Even when the concentration of CDDP was low, CDDP significantly decreased the proliferation of STKM-1 human stomach cancer cells, thus confirming the synergistic effect of the CDDP and caffeine group. The apoptotic labeling index of the CDDP plus caffeine combination was significantly higher than that of the CDDP group. In conclusion, caffeine enhanced the effect of CDDP by not only inhibiting DNA repairs but also inducing apoptosis.
Takahashi
M
M
First Department of Surgery, Yokohama City University, School of Medicine, Japan.
Yamamoto
Y
Y
Hatori
S
S
Shiozawa
M
M
Suzuki
M
M
Rino
Y
Y
Amano
T
T
Imada
T
T
D017209:Apoptosis / Q000187:drug effects; D002110:Caffeine / Q000633:toxicity*; D002455:Cell Division / Q000187:drug effects; D002945:Cisplatin / Q000633:toxicity*; D004357:Drug Synergism; D006801:Humans; D008940:Mitotic Index / Q000187:drug effects; D013274:Stomach Neoplasms / Q000473:pathology*; D014407:Tumor Cells, Cultured
D002110:Caffeine; D002945:Cisplatin