Immunoreactivity and in-vitro effect on human sperm-egg binding of antibodies against peptides corresponding to bonnet monkey zona pellucida-3 glycoprotein.
The following synthetic peptides were made as immunogens for development of a zona-based contraceptive vaccine: P1, KQPFWLLQGGASRAETSVQPVLVE [amino acids (aa) 23-45 with an additional K at the N-terminus]; P2, FSEEKLVFSLRLMEENC (aa 164-179 with an additional C at the C-terminus and T170 replaced by V); and P3, CSFSKSSNSWFPVEGPADICQCC (aa 300-322). The aa are numbered on the basis of bonnet monkey ZP3 precursor protein. Antibodies against an additional peptide P4, KGDCGTPSHSRRQPHVVSQWSRSA (aa 324-347), significantly inhibits human sperm-oocyte binding. In addition, antibodies against cocktail of peptide-diphtheria toxoid conjugates also significantly inhibit the binding of spermatozoa to zona pellucida in a hemizona assay. These results will further help in the design of an immunocontraceptive vaccine based upon synthetic peptides corresponding to ZP3.
Afzalpurkar
A
A
Gamete Antigen Laboratory, National Institute of Immunology, New Delhi, India.
Shibahara
H
H
Hasegawa
A
A
Koyama
K
K
Gupta
S K
SK
D000595:Amino Acid Sequence; D000818:Animals; D003269:Contraception, Immunologic; D004168:Diphtheria Toxoid / Q000276:immunology; D004527:Egg Proteins / Q000737:chemistry / Q000276:immunology*; D005260:Female; D019084:Fluorescent Antibody Technique, Indirect; D006801:Humans; D007106:Immune Sera / Q000276:immunology / Q000494:pharmacology*; D007114:Immunization; D008255:Macaca radiata / Q000276:immunology; D008297:Male; D008562:Membrane Glycoproteins / Q000737:chemistry / Q000276:immunology*; D008969:Molecular Sequence Data; D011956:Receptors, Cell Surface; D013084:Sperm-Ovum Interactions / Q000187:drug effects*; D014612:Vaccines; D015044:Zona Pellucida / Q000276:immunology*; D000072216:Zona Pellucida Glycoproteins
D004168:Diphtheria Toxoid; D004527:Egg Proteins; D007106:Immune Sera; D008562:Membrane Glycoproteins; D011956:Receptors, Cell Surface; D014612:Vaccines; C000606517:ZP3 protein, human; D000072216:Zona Pellucida Glycoproteins