The IPCS Collaborative Study on Neurobehavioral Screening Methods: V. Results of chemical testing. Steering Group.
The IPCS Collaborative Study on Neurobehavioral Screening Methods was undertaken to determine the intra- and inter-laboratory reliability of a functional observational battery (FOB) and an automated assessment of motor activity in eight laboratories world-wide. Following the training phase and the conduct of proficiency studies in all laboratories, participants proceeded to test the effects of seven chemicals in both single dose and four-week repeated dosing scenarios. The chemicals studied were acrylamide, bisacrylamide, p,p'-DDT, lead acetate, parathion, toluene, and triethyl tin. Participants received coded samples from a common source. In order to judge the general utility of these procedures in a diversity of testing situations, laboratories conducted the studies under their standard conditions, using their choice of rat strain and test equipment. Chemical does and time of peak effect for acute testing were determined by each laboratory: these parameters were quite similar for some chemicals, but varied greatly for others. The results of the chemical tests indicated that while there was some variability in the data on specific endpoints, all laboratories detected and characterized the effects of all but one of the known neurotoxicants. The one exception (toluene) was probably due to other factors (e.g., dose level, route of administration) rather than lack of sensitivity of the test methods. This study provides extensive data regarding the use of neurobehavioral screening methods over a range of laboratory conditions as well as the reliability, sensitivity, and robustness of the tests to detect neurotoxic potential of chemicals.
Moser
V C
VC
Neurotoxicology Division, U.S. Environmental Protection Agency, Research Triangle Park, North Carolina 27711 USA.
Becking
G C
GC
Cuomo
V
V
Frantík
E
E
Kulig
B M
BM
MacPhail
R C
RC
Tilson
H A
HA
Winneke
G
G
Brightwell
W S
WS
De Salvia
M A
MA
Gill
M W
MW
Haggerty
G C
GC
Hornychová
M
M
Lammers
J
J
Larsen
J J
JJ
McDaniel
K L
KL
Nelson
B K
BK
Ostergaard
G
G
D000818:Animals; D001522:Behavior, Animal / Q000187:drug effects*; D001921:Brain / Q000187:drug effects* / Q000503:physiopathology; D004353:Drug Evaluation, Preclinical / Q000379:methods; D017408:Guidelines as Topic; D008297:Male; D051381:Rats; D015203:Reproducibility of Results; D018675:Toxicity Tests / Q000379:methods*; D015262:Xenobiotics / Q000633:toxicity*
D015262:Xenobiotics