Detection of endogenous retrovirus antigens in NOD mouse pancreatic beta-cells.
We characterized C-type retroviruses expressed in the pancreatic beta-cells of non-obese diabetic (NOD) mice by immunohistochemical techniques and by inhibiting the production of viral particles using antisense oligonucleotides. Some cells in the pancreatic islets from both NOD and diabetes-resistant NOD-related mice (NON) reacted with a monoclonal antibody directed against the envelope protein(s) of polytropic viruses. On the other hand, NOD islet cells also showed strong immunoreactivity with an anti-gag protein monoclonal antibody and another anti-envelope protein(s) monoclonal antibody that is specific for xenotropic viruses. In antisense oligodeoxynucleotide inhibition assays, a xenotropic virus-specific phosphorothionate-particles antisense oligodeoxynucleotide significantly inhibited the occurrence of C-type virus particles in NOD mouse islet beta-cells. Therefore, C-type retrovirus-like particles expressed in NOD mouse pancreatic beta-cells were considered to be endogenous xenotropic virus. The expression of the xenotropic viral genome may be involved in the pathogenesis of the diabetic syndrome in NOD mice.
Tsumura
H
H
Institute of Laboratory Animals, Mie University School of Medicine, Japan.
Miyazawa
M
M
Ogawa
S
S
Wang
J Z
JZ
Ito
Y
Y
Shimura
K
K
D000818:Animals; D000910:Antibodies, Heterophile / Q000494:pharmacology; D000911:Antibodies, Monoclonal / Q000276:immunology; D000956:Antigens, Viral / Q000032:analysis* / Q000276:immunology; D003922:Diabetes Mellitus, Type 1 / Q000821:virology*; D005260:Female; D017988:Gammaretrovirus / Q000235:genetics / Q000276:immunology* / Q000502:physiology; D015686:Gene Products, env / Q000032:analysis; D015683:Gene Products, gag / Q000032:analysis; D007124:Immunoenzyme Techniques; D007515:Islets of Langerhans / Q000276:immunology / Q000473:pathology / Q000821:virology*; D008297:Male; D051379:Mice; D016688:Mice, Inbred NOD; D008854:Microscopy, Electron; D008856:Microscopy, Fluorescence; D016376:Oligonucleotides, Antisense / Q000737:chemistry / Q000276:immunology / Q000494:pharmacology; D013047:Specific Pathogen-Free Organisms; D013873:Thionucleotides / Q000276:immunology / Q000494:pharmacology; D014771:Virion / Q000235:genetics / Q000276:immunology* / Q000502:physiology
D000910:Antibodies, Heterophile; D000911:Antibodies, Monoclonal; D000956:Antigens, Viral; D015686:Gene Products, env; D015683:Gene Products, gag; D016376:Oligonucleotides, Antisense; D013873:Thionucleotides