Capture of HIV-1 gp120 and virions by lectin-immobilized polystyrene nanospheres.
A lectin such as concanavalin A (Con A) was immobilized on the surfaces of poly(methacrylic acid) branches covered with polystyrene nanospheres with a diameter of 360 nm, which were obtained by the free radical copolymerization of styrene with the poly(tert-butylmethacrylate) macromonomer, followed by hydrolysis. Using Con A-immobilized (0.3 microgram/cm2) nanospheres, the interaction of the nanospheres with HIV-1 was determined by the reduction of the gp120 level and the viral infectivity of the HIV-1 suspensions after a 60 min incubation at room temperatures. Con A-immobilized nanospheres achieved a 95 and a 77% reduction of the gp120 level and the infectivity at a concentration of 0.5 mg/mL, respectively, indicating the effective capture of the gp120 and the virions.
Akashi
M
M
Department of Applied Chemistry and Chemical Engineering, Faculty of Engineering, Kagoshima University, Japan.
Niikawa
T
T
Serizawa
T
T
Hayakawa
T
T
Baba
M
M
D003208:Concanavalin A; D015699:HIV Envelope Protein gp120 / Q000302:isolation & purification* / Q000378:metabolism; D015497:HIV-1 / Q000737:chemistry / Q000302:isolation & purification*; D037102:Lectins / Q000378:metabolism; D008358:Mannose / Q000737:chemistry / Q000378:metabolism; D008863:Microspheres; D011137:Polystyrenes; D014771:Virion / Q000302:isolation & purification*
D015699:HIV Envelope Protein gp120; D037102:Lectins; D011137:Polystyrenes; D003208:Concanavalin A; D008358:Mannose