title: Nutrient-gene interactions determine mitochondrial function: effect of dietary fat.
abstract: The effect on mitochondrial respiration of feeding hydrogenated coconut oil, corn oil, or menhaden oil (MO) to diabetes-prone BHE/cdb rats and normal Sprague Dawley (SD) rats was studied. Both fat source and strain affected the temperature dependence of succinate-supported respiration. The transition temperature was greater in BHE/cdb rats than in the SD rats. The efficiency of ATP synthesis as reflected by the ADP:O ratio was decreased in the BHE/cdb rats compared to SD rats, with the exception of the comparison made at 37 degrees C with the MO-fed rats; at this temperature, the ADP:O ratios were similar. The diet and strain differences suggest a dietary lipid-gene interaction with respect to the mobility of subunit 6 of the F1F0ATPase. This subunit has two errors in its gene: one that affects the proton channel and another that likely affects its mobility within the inner mitochondrial membrane.
authors.affiliation: Department of Foods and Nutrition, University of Georgia, Athens 30602-3622, USA.
authors.forename: M J
authors.initials: MJ
authors.lastname: Kim
authors.forename: C D
authors.initials: CD
authors.lastname: Berdanier
mesh_terms: D000818:Animals; D000074263:Coconut Oil; D003059:Cocos; D003314:Corn Oil / Q000494:pharmacology; D003924:Diabetes Mellitus, Type 2 / Q000235:genetics / Q000378:metabolism; D004041:Dietary Fats / Q000494:pharmacology*; D004198:Disease Susceptibility; D005395:Fish Oils / Q000494:pharmacology; D007425:Intracellular Membranes / Q000378:metabolism; D007700:Kinetics; D008297:Male; D008930:Mitochondria, Liver / Q000378:metabolism*; D010085:Oxidative Phosphorylation; D010101:Oxygen Consumption / Q000187:drug effects; D010938:Plant Oils / Q000494:pharmacology; D006180:Proton-Translocating ATPases / Q000235:genetics* / Q000378:metabolism; D051381:Rats; D011922:Rats, Mutant Strains; D017207:Rats, Sprague-Dawley; D013816:Thermodynamics
chemical_list: D004041:Dietary Fats; D005395:Fish Oils; D010938:Plant Oils; C033266:Menhaden oil; D003314:Corn Oil; D006180:Proton-Translocating ATPases; D000074263:Coconut Oil