Analysis of electrical phenomena accompanying the growth of Neurospora crassa hyphae: theory and experiment.
To describe electrical phenomena observed in growth of Neurospora crassa hyphae, a theoretical model was developed considering the hypha as a one-dimensional electric cable with non-uniform longitudinal distribution of current sources reflecting the activity of proton pumps. A profile of the density of the pump current along the hypha is proposed, at which the results of simulation quantitatively coincide with the results of physiological experiments. The model values of energy coupling in the growth zones were estimated. The experimental dependence of the elongation rate of regenerating apical hypha fragments on their lengths was determined. Based on the comparison of these experimental results with the results of analysis of the model, the contribution of the axial metabolite transport, from the distal parts of the hypha to the apical part, to the dynamics of the apical cell growth was estimated. The possibility of evaluating the intensity of high-molecular-weight syntheses and/or accumulation of substances in granules was demonstrated. The growth rate of the regenerating hypha fragments was shown to correlate with the electric current flowing into the apical fragment 0.2-mm in length.
Aslanidi
K B
KB
Institute of Theoretical and Experimental Biophysics, Russian Academy of Sciences, Pushchino, Moscow Region, Russia. aslanidi@venus.iteb.serpukhov.su
Aslanidi
O V
OV
Vachadze
D M
DM
Mornev
O A
OA
Potapova
T V
TV
Chailakhyan
L M
LM
Shtemanetyan
E G
EG
D002462:Cell Membrane / Q000502:physiology; D004553:Electric Conductivity; D004735:Energy Transfer; D008564:Membrane Potentials; D008954:Models, Biological; D009492:Neurospora crassa / Q000254:growth & development / Q000502:physiology*; D017494:Proton Pumps
D017494:Proton Pumps