title: Rapid detection of D-dimer using a fiber optic biosensor.
abstract: We describe a rapid and sensitive method for detection and quantification of D-dimer and other crosslinked fibrin degradation products (XL-FDPs), which are present in elevated concentrations in patients with sepsis and thrombotic disorders. The method utilizes a sandwich fluoroimmunoassay immobilized in the sensing region of an evanescent wave biosensor. Physiological concentrations of D-dimer and high molecular weight XL-FDP could be determined in buffer and plasma samples on calibrated fibers in 11 min. Samples from septic patients were assayed using ELISA and the fiber optic method; concentrations determined by fiber optic assay were strongly correlated with those determined by ELISA (r = 0.918); intra- and inter-assay errors were comparable to those from ELISAs. Given its accuracy and rapid response time, this fiber optic biosensor shows great potential for development as a diagnostic tool.
authors.affiliation: Naval Research Laboratory, Center for Bio/Molecular Science and Engineering, Washington, DC 20375-5438, USA.
authors.forename: C A
authors.initials: CA
authors.lastname: Rowe
authors.forename: J S
authors.initials: JS
authors.lastname: Bolitho
authors.forename: A
authors.initials: A
authors.lastname: Jane
authors.forename: P G
authors.initials: PG
authors.lastname: Bundesen
authors.forename: D B
authors.initials: DB
authors.lastname: Rylatt
authors.forename: P R
authors.initials: PR
authors.lastname: Eisenberg
authors.forename: F S
authors.initials: FS
authors.lastname: Ligler
mesh_terms: D015374:Biosensing Techniques; D002021:Buffers; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D005336:Fiber Optic Technology; D005338:Fibrin Fibrinogen Degradation Products / Q000032:analysis*; D006801:Humans; D008297:Male; D008970:Molecular Weight; D055100:Optical Fibers; D012015:Reference Standards; D012016:Reference Values; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013997:Time Factors
chemical_list: D002021:Buffers; D005338:Fibrin Fibrinogen Degradation Products; C036309:fibrin fragment D