Analysis of cell kinetics after gamma-ray irradiation using an anti-BrdU monoclonal antibody.
The cell cycle was analyzed using double staining with an anti-bromodeoxyuridine (BrdU) monoclonal antibody and propidium iodide (PI). Changes in cell kinetics after irradiation were compared with those seen by the conventional PI-based DNA histogram method. The effect of irradiation on cell kinetics has been studied primarily by counting G2-arrested cells. By the present BrdU method, a rapid transition from G1 to S-phase was observed within 2 h of irradiation, followed by G1 block. Cells in the S-phase progressed to G2+M where they arrested, resulting in a decreased percentage of S cells (<5%). Release of G1 block occurred after 8 h, and G2+M cells returned to G1 after >18 h. The initial G1 arrest induced by irradiation was confirmed for the first time by the present BrdU-PI double staining.
Murata
T
T
Department of Radiology, Kansai Medical University, Osaka, Japan.
Akagi
K
K
Nasu
R
R
Kimura
H
H
Tanaka
Y
Y
D000911:Antibodies, Monoclonal; D001973:Bromodeoxyuridine / Q000276:immunology; D002453:Cell Cycle / Q000528:radiation effects*; D004247:DNA / Q000096:biosynthesis; D005434:Flow Cytometry; D019084:Fluorescent Antibody Technique, Indirect; D005720:Gamma Rays; D006367:HeLa Cells / Q000502:physiology; D006801:Humans; D007399:Interphase / Q000528:radiation effects; D011419:Propidium
D000911:Antibodies, Monoclonal; D011419:Propidium; D004247:DNA; D001973:Bromodeoxyuridine