title: Characterization of a molecular clone of HIV type 2 infectious for Macaca nemestrina.
abstract: A lambda phage clone containing a full-length HIV-2 provirus, designated HIV-2KR, was obtained from the genomic DNA of Molt4 clone 8 (Molt4/8) lymphoblastic cells infected with the HIV-2PEI2 strain. HIV-2KR is genetically distinct from known HIV-2 isolates, possessing both a unique deletion in the LTR promoter region, and a long rev reading frame. It is replication competent in vitro after transfection into Molt4/8 cells, replicates in a variety of established human T lymphoblastic (Molt-3, Molt4/8, SupT1, H9, C8166) and myelomonocytic (U937) cell lines, and displays prominent cytopathic effects on infection of Molt4/8 cells, reflecting usage of both CCR5 and CXCR4 coreceptors. In addition, HIV-2KR was found to be infectious for human and Macaca nemestrina peripheral blood lymphocytes, and primary human monocyte-macrophage cultures. Intravenous inoculation of cell-free virus into M. nemestrina resulted in infection characterized by transient, low-level viremia and modest temporary decline in CD4 lymphocyte numbers, making HIV-2KR the first HIV-2 molecular clone reported to be infectious for this primate species.
authors.affiliation: Department of Biology, University of California-San Diego, La Jolla 92093, USA.
authors.forename: G
authors.initials: G
authors.lastname: Kraus
authors.forename: A
authors.initials: A
authors.lastname: Radaelli
authors.forename: R
authors.initials: R
authors.lastname: Talbott
authors.forename: M
authors.initials: M
authors.lastname: Leavitt
authors.forename: A
authors.initials: A
authors.lastname: Schmidt
authors.forename: P
authors.initials: P
authors.lastname: Badel
authors.forename: C
authors.initials: C
authors.lastname: Bartz
authors.forename: W
authors.initials: W
authors.lastname: Morton
authors.forename: F
authors.initials: F
authors.lastname: Wong-Staal
authors.forename: D J
authors.initials: DJ
authors.lastname: Looney
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D004195:Disease Models, Animal; D015658:HIV Infections / Q000821:virology*; D015498:HIV-2 / Q000145:classification / Q000235:genetics* / Q000472:pathogenicity; D006801:Humans; D008254:Macaca nemestrina; D008969:Molecular Sequence Data; D010641:Phenotype; D010802:Phylogeny; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid