title: [HIV infection in a seronegative child diagnosed by isolation of the virus. The significant immunological and epidemiological signs].
abstract: HIV-1, subtype F was isolated from a seronegative child aged 2.5 yr. ELISA tests (Behring HIV-1 + 2, Abbott HIV-1 + 2, Wellcozyme HIV-1 Recombinant, Clonatec HIV-1 + 2, Genelavia Mixt), and also rapid tests (Abbott Pack, Serodia) were all negative, although some of them presented borderline reactivities. Western Blot (Cambridge Biotech) revealed an undetermined profile (traces of anti-gp160 plus anti-p24). A new WB test (Sanofi Dg. Pasteur) performed at a higher serum concentration (1/25) revealed a complete antibody profile, despite the very low intensity of bands. A new serum sample prelevated 6 month later was completely negative on all tests used. Both samples, were negatives in WB for HIV2 and HTLVs. A heparinised blood sample was used for the co-cultivation of PBMC and was proven to be positive in the 14th day of culture. The isolated DNA from end-culture cells was subjected to PCR amplifications for Heteroduplex Mobility Assay direct subtyping (primers ES7 and ES8) and for the investigation of genotypic sensitivity to AZT (primers A/NE1). Lymphocyte populations phenotyping revealed leukocytosis (> 15,000/mL) with a predominance of the CD8+ subset CD4/CD8 ratio was < 1. Plasmatic HIV-1 load (measured by bDNA--Chiron) did not reached detectable levels of HIV-1 RNA. p24 Ag assay (EIA-Coulter) revealed a detectable p24 antigenemia only in the first serum sample and only after acid dissociation. So, this patient may present an integrated HIV-1 infection until now "silent".
authors.affiliation: Disciplina de Microbiologie, Universitatea de Medicină şi Farmacie Gr. T. Popa, Iaşi.
authors.forename: C
authors.initials: C
authors.lastname: Apetrei
authors.forename: D
authors.initials: D
authors.lastname: Grămadă
authors.forename: V
authors.initials: V
authors.lastname: Stanciu
authors.forename: D
authors.initials: D
authors.lastname: Diculencu
authors.forename: C
authors.initials: C
authors.lastname: Pânzaru
authors.forename: G
authors.initials: G
authors.lastname: Coman
authors.forename: E
authors.initials: E
authors.lastname: Carasievici
authors.forename: M
authors.initials: M
authors.lastname: Duca
mesh_terms: D015492:AIDS Serodiagnosis / Q000379:methods / Q000706:statistics & numerical data; D002675:Child, Preschool; D015658:HIV Infections / Q000175:diagnosis* / Q000453:epidemiology / Q000276:immunology / Q000821:virology; D018023:HIV Seronegativity / Q000276:immunology*; D015497:HIV-1 / Q000302:isolation & purification*; D006801:Humans; D008297:Male; D016036:Seroepidemiologic Studies; D019562:Viral Load; D014766:Viremia / Q000175:diagnosis
vernacular_title: Infecţie cu HIV la un copil seronegativ diagnosticată prin izolarea virusului. Semnificaţii imunologice şi epidemiologice.