Mapping of the human genes encoding cyclin H (CCNH) and the CDK-activating kinase (CAK) assembly factor MAT1 (MNAT1) to chromosome bands 5q13.3-q14 and 14q23, respectively.
Cyclin-dependent kinases (CDKs), which play a key role in cell cycle control, are activated by the CDK-activating kinase (CAK), which activates cyclin-bound CDKs by phosphorylation at the specific threonine residue. Mammalian CAK contains three components: CDK7, cyclin H, and an assembly factor called MAT1. The CDK7-cyclin H-MAT1 complex is tightly associated with a multiprotein complex TFIIH, which plays a dual role in transcription and DNA repair. Here, we have determined chromosomal localizations of the human genes encoding cyclin H (CCNH) and MAT1 (HGMW-approved symbol MNAT1) to chromosome bands 5q13.3-q14 and 14q23, respectively, by using fluorescence in situ hybridization, somatic cell hybrid analyses, and mapping to the human YAC contigs.
Eki
T
T
Cellular Physiology Laboratory, Tsukuba Life Science Center, Ibaraki, Japan. eki@rtc.riken.go.jp
Okumura
K
K
Abe
M
M
Kagotani
K
K
Taguchi
H
H
Murakami
Y
Y
Pan
Z Q
ZQ
Hanaoka
F
F
D000818:Animals; D001483:Base Sequence; D015139:Blotting, Southern; D002352:Carrier Proteins; D018797:Cell Cycle Proteins; D002874:Chromosome Mapping; D018244:Chromosomes, Artificial, Yeast; D002883:Chromosomes, Human, Pair 14; D002895:Chromosomes, Human, Pair 5; D006224:Cricetinae; D056748:Cyclin H; D018844:Cyclin-Dependent Kinases; D016213:Cyclins / Q000235:genetics*; D005819:Genetic Markers; D006801:Humans; D006822:Hybrid Cells; D017404:In Situ Hybridization, Fluorescence; D051379:Mice; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D017346:Protein Serine-Threonine Kinases / Q000235:genetics*; D014157:Transcription Factors; D000095043:Cyclin-Dependent Kinase-Activating Kinase
C534751:CCNH protein, human; D002352:Carrier Proteins; C534752:Ccnh protein, mouse; D018797:Cell Cycle Proteins; D056748:Cyclin H; D016213:Cyclins; D005819:Genetic Markers; C514215:MNAT1 protein, human; D014157:Transcription Factors; D017346:Protein Serine-Threonine Kinases; D018844:Cyclin-Dependent Kinases; D000095043:Cyclin-Dependent Kinase-Activating Kinase