title: The involvement of the GH/IGF-I axis in the regulation of secretory activity by bovine oviduct epithelial cells.
abstract: The aim of this study was to investigate the role of the GH/IGF-I system in the control of secretory activity by oviductal cells. The effects of GH (1, 10, 100 and 1000 ng/ml medium) on IGF-I production, as well as the action of GH and IGF-I (1, 10, 100 ng/ml medium) on cAMP and prostaglandin F alpha (PGF) secretion by cultured bovine oviduct epithelial cells were studied. It was observed that GH at all doses tested significantly (p < 0.05) increased IGF-I secretion. Moreover, both GH and IGF-I significantly stimulated cAMP production by oviductal cells. On the other hand, PGF release was significantly (p < 0.05) inhibited by both GH and IGF-I at all concentrations tested. Our results demonstrate that: (1) oviductal cells can produce PGF alpha, cAMP and IGF-I, (2) production of PGF alpha is under inhibitory regulation by GH and IGF-I, (3) GH/IGF can activate cAMP-dependent intracellular mechanisms, which might be the mediators of GH/IGF-I action on oviductal cells. These observations confirm the involvement of GH/IGF-I system in control of oviductal prostaglandin and cyclic nucleotide secretion.
authors.affiliation: Research Institute of Animal Production, Nitra, Slovak Republic. makarev@vuzu.sk
authors.forename: A V
authors.initials: AV
authors.lastname: Makarevich
authors.forename: A V
authors.initials: AV
authors.lastname: Sirotkin
mesh_terms: D000818:Animals; D002417:Cattle; D002478:Cells, Cultured; D000242:Cyclic AMP / Q000378:metabolism*; D004305:Dose-Response Relationship, Drug; D004847:Epithelial Cells / Q000187:drug effects / Q000502:physiology; D005187:Fallopian Tubes / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D005260:Female; D013006:Growth Hormone / Q000494:pharmacology*; D007334:Insulin-Like Growth Factor I / Q000187:drug effects / Q000378:metabolism* / Q000494:pharmacology; D011460:Prostaglandins F / Q000378:metabolism*; D011863:Radioimmunoassay / Q000662:veterinary; D015203:Reproducibility of Results
chemical_list: D011460:Prostaglandins F; D007334:Insulin-Like Growth Factor I; D013006:Growth Hormone; D000242:Cyclic AMP