Differentiation of Arthroderma spp. by random amplification of polymorphic DNA (RAPD) and Southern hybridization.
To develop the molecular differentiation analysis of dermatophytes, we carried out RAPD and Southern hybridization analyses using genomic DNAs of six Arthroderma species, including A. fulvum, A. grubyi, A. gypseum, A. incurvatum, A. otae and A. racemosum. The RAPD analysis gave different band patterns specific to each of the six Arthroderma fungi. However, minor differences in the banding patterns were observed between the strains of plus (+) and minus (-) mating types of A. gypseum, A. fulvum and A. incurvatum. Southern blot analysis using a probe (1S) obtained from A. grubyi DNA gave specific bands only in the DNA samples of A. grubyi and A. incurvatum. On the other hand, Southern blot analysis using a probe (C3) obtained from A. otae DNA gave specific bands in all six Arthroderma species examined, and the size of the bands were specific to each species. These findings indicate that RAPD and Southern hybridization analyses are useful in the differentiation of these Arthroderma species.
Kano
R
R
Department of Veterinary Internal Medicine, Faculty of Agriculture, University of Tokyo, Japan.
Matshushiro
H
H
Watari
T
T
Tsujimoto
H
H
Hasegawa
A
A
D000595:Amino Acid Sequence; D001203:Ascomycota / Q000145:classification* / Q000235:genetics*; D001483:Base Sequence; D015139:Blotting, Southern / Q000379:methods; D015342:DNA Probes; D004271:DNA, Fungal / Q000032:analysis*; D008969:Molecular Sequence Data; D016533:Mycological Typing Techniques; D019105:Random Amplified Polymorphic DNA Technique; D013045:Species Specificity
D015342:DNA Probes; D004271:DNA, Fungal