[Gas chromatographic determination of high molecular weight nonesterified fatty acids in biological material].
A quick and precise method has been proposed that permits qualitatively determining high-molecular fatty acids which are contained in biological material at nonesterified state. The method includes the extraction of lipids by chloroform-methanol mixture, deposition of nonesterified fatty acids in the lipid extract by the use of sodium methylate, their release from phospholipids, triacyl glycerols, free and ester-bound cholesterol, isolation in pure form, methylation and gas-liquid chromatography of methyl esters of fatty acids. The method advantage consists in minimum quantities of the added inner standard to the studied material before the extraction of lipids and possibility of determination of nonesterified fatty acids in any biological sample.
Ribis
I F
IF
Danylyk
B B
BB
D000818:Animals; D001826:Body Fluids / Q000737:chemistry*; D002849:Chromatography, Gas; D005230:Fatty Acids, Nonesterified / Q000032:analysis*; D008055:Lipids / Q000302:isolation & purification; D008970:Molecular Weight; D010944:Plants / Q000737:chemistry*; D015203:Reproducibility of Results
D005230:Fatty Acids, Nonesterified; D008055:Lipids
Gazokhromatohrafichne vyznachennia vysokomolekuliarnykh neeterifikovanykh zhyrnykh kyslot u biolohichnomu materiali.