Genomic organization of the human Y-box protein (YB-1) gene.
The human Y-box protein (YB-1) is a member of a family of DNA-binding proteins containing a highly conserved cold shock domain. The genomic organization of the human YB-1 gene was determined from five overlapping genomic clones that encompassed all exons of the gene. Sequence analysis of these clones revealed that human YB-1 spans approximately 19 kb of genomic DNA and contains eight exons. The cold shock domain is encoded by exons 1-5. Both exon-splitting and codon-splitting in the region of the gene encoding the cold shock domain are similar to those in the corresponding region of another Y-box binding protein, dbpA. Exon-intron structures and nucleotide sequences of the regions encoding the N-terminal and C-terminal domains of the two proteins differ markedly between YB-1 and dbpA. These observations suggest that YB-1 and dbpA arose by duplication of a common ancestral gene encoding all these domains.
Toh
S
S
Department of Biochemistry, Kyushu University School of Medicine, Maidashi, Fukuoka, Japan.
Nakamura
T
T
Ohga
T
T
Koike
K
K
Uchiumi
T
T
Wada
M
M
Kuwano
M
M
Kohno
K
K
D001483:Base Sequence; D015139:Blotting, Southern; D022762:CCAAT-Enhancer-Binding Proteins; D002352:Carrier Proteins; D004268:DNA-Binding Proteins / Q000235:genetics*; D005091:Exons; D015894:Genome, Human; D006360:Heat-Shock Proteins / Q000235:genetics; D006801:Humans; D007438:Introns; D008969:Molecular Sequence Data; D051839:NFI Transcription Factors; D009687:Nuclear Proteins; D011544:Pseudogenes; D012326:RNA Splicing; D014157:Transcription Factors; D051840:Y-Box-Binding Protein 1
D022762:CCAAT-Enhancer-Binding Proteins; D002352:Carrier Proteins; D004268:DNA-Binding Proteins; D006360:Heat-Shock Proteins; D051839:NFI Transcription Factors; D009687:Nuclear Proteins; D014157:Transcription Factors; D051840:Y-Box-Binding Protein 1; C070856:YBX1 protein, human; C094867:YBX3 protein, human