title: Resistance to anoxic injury in the dorsal columns of adult rat spinal cord following demyelination.
abstract: In order to examine the relationship between myelination and sensitivity to anoxia in adult white matter, we studied action potential conduction in the spinal cord dorsal column of adult rats in which focal demyelinating lesions had been produced using ethidium bromide/X-irradiation. Acutely isolated spinal cords from control rats and following demyelination were maintained in vitro at 36 degrees C and compound action potentials were studied following supramaximal stimulation. The compound action potential was totally abolished within 12 min of the onset of anoxia in normal dorsal columns, but was not abolished until 50 min following the onset of anoxia in demyelinated dorsal columns. Compound action potentials showed significantly greater recovery (to 58.1 +/- 12.2% of control amplitude) in demyelinated dorsal columns compared to controls (30.8 +/- 5.3%) following 120 min of reoxygenation. These results show that focal demyelination is associated with reduced sensitivity to anoxia within white matter of the adult spinal cord.
authors.affiliation: Department of Neurology, Yale University School of Medicine, New Haven, CT 06510, USA.
authors.forename: T
authors.initials: T
authors.lastname: Imaizumi
authors.forename: J D
authors.initials: JD
authors.lastname: Kocsis
authors.forename: S G
authors.initials: SG
authors.lastname: Waxman
mesh_terms: D000200:Action Potentials / Q000502:physiology; D000818:Animals; D003711:Demyelinating Diseases / Q000503:physiopathology*; D005260:Female; D000860:Hypoxia / Q000503:physiopathology*; D009431:Neural Conduction / Q000502:physiology*; D051381:Rats; D017208:Rats, Wistar; D013118:Spinal Cord Diseases / Q000503:physiopathology*