Tex261, a novel gene presumably related but distinct from steroidogenic acute regulatory (StAR) gene, is regulated during the development of germ cells.
Tex261 is a new gene cloned from a subtractive cDNA library from 10-day postnatal mouse testis. Tex261 transcribes three mRNAs of 3.5, 1.6 and 1.4 kb. The 3.5 kb and 1.4 kb transcripts are expressed in different gonadal and somatic tissues analyzed. However, the 1.6 Kb transcript is only detected in testis and differentially regulated during development. This 1.6 kb mRNA is highly expressed in adult testis, with detection beginning at 15 days of postnatal life, which coincides with the presence of pachytene cells in prepuberal mouse. This expression was confirmed in pachytene cells by run-off transcription assay and by in situ hybridization. A region of 86 amino acids from the predicted Tex261 was recently reported as a part of the steroidogenic acute regulatory protein StAR gene by its sequence identity to a rat StAR cloned cDNA. We demonstrate her that, in the mouse, StAR and Tex261 are two different genes with different expected functions, yet, a high identity (43%) at amino acid level is detected in a region of 153 amino acids corresponding to a transmembrane protein.
López-Fernández
L A
LA
Department of Cell and Developmental Biology, Centro de Investigaciones Biológicas (C.S.I.C.), Madrid, Spain.
Párraga
M
M
del Mazo
J
J
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D015152:Blotting, Northern; D003001:Cloning, Molecular; D018507:Gene Expression Regulation, Developmental / Q000235:genetics*; D005854:Germ Cells / Q000254:growth & development*; D017403:In Situ Hybridization; D008297:Male; D051379:Mice; D008969:Molecular Sequence Data; D051903:Nuclear Factor 45 Protein; D010750:Phosphoproteins / Q000235:genetics*; D015347:RNA Probes; D012333:RNA, Messenger / Q000378:metabolism; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D013737:Testis / Q000737:chemistry* / Q000166:cytology; D014158:Transcription, Genetic / Q000235:genetics; D000098682:Steroidogenic Acute Regulatory Protein
C494014:Ilf2 protein, mouse; D051903:Nuclear Factor 45 Protein; D010750:Phosphoproteins; D015347:RNA Probes; D012333:RNA, Messenger; D000098682:Steroidogenic Acute Regulatory Protein