Antimutagenicity, cytotoxicity and composition of chlorophyllin copper complex.
The preparation of chlorophyllin copper complex (CCC), shown to be a tumor promoter in an animal model (Nelson, R.L. (1992) Chlorophyllin, an antimutagen, acts as a tumor promoter in the rat-dimethylhydrazine colon carcinogenesis model. Anticancer Res., 12, 737-740), also inhibits the activities of direct- and indirect-acting mutagens in the Salmonella assay and exhibits cytostatic and cytocidal effects toward myeloma cells. Data from elemental analyses, spectrophotometry and reversed-phase high-performance liquid chromatography indicate that CCC preparations generally used in antimutagenic/anticarcinogenic experiments are variable, complex mixtures of structurally distinct porphyrins lacking copper in some instances. This variability of the composition may be a cause for the differences reported for the tumor promotion activity of CCC.
Chernomorsky
S
S
Department of Biochemistry, Rutgers University, New Brunswick, NJ 08903-0231, USA.
Rancourt
R
R
Virdi
K
K
Segelman
A
A
Poretz
R D
RD
D000818:Animals; D016587:Antimutagenic Agents / Q000494:pharmacology*; D000970:Antineoplastic Agents / Q000737:chemistry / Q000494:pharmacology*; D002455:Cell Division / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D002735:Chlorophyllides / Q000737:chemistry* / Q000494:pharmacology*; D002851:Chromatography, High Pressure Liquid; D003300:Copper / Q000032:analysis; D004305:Dose-Response Relationship, Drug; D051379:Mice; D010954:Plasmacytoma / Q000473:pathology; D012486:Salmonella typhimurium / Q000187:drug effects; D013053:Spectrophotometry; D014407:Tumor Cells, Cultured
D016587:Antimutagenic Agents; D000970:Antineoplastic Agents; D002735:Chlorophyllides; D003300:Copper; C007020:chlorophyllin