Adeno-associated virus gene transfer to mouse retina.
Ocular gene transfer may provide a means for arresting the retinal degeneration characteristic of many inherited causes of blindness, including retinitis pigmentosa (RP). Previously, we have shown in immunodeficient animals that recombinant adeno-associated virus (rAAV) mediates transduction of photoreceptors as well as the retinal pigment epithelium (RPE) following subretinal injection. In this study we extend these observations and show that highly purified recombinant AAV vectors encoding the reporter gene LacZ transduce photoreceptors in an immunocompetent mouse strain following subretinal injection and efficiently transduce ganglion cells after intravitreal injection. Levels of transduction increase over time. Sublethal gamma-irradiation is shown to facilitate this process.
Ali
R R
RR
Department of Molecular Genetics, Institute of Ophthalmology, University College London, UK.
Reichel
M B
MB
De Alwis
M
M
Kanuga
N
N
Kinnon
C
C
Levinsky
R J
RJ
Hunt
D M
DM
Bhattacharya
S S
SS
Thrasher
A J
AJ
D000818:Animals; D000229:Dependovirus / Q000235:genetics*; D005720:Gamma Rays; D018014:Gene Transfer Techniques; D005822:Genetic Vectors / Q000235:genetics; D007763:Lac Operon / Q000235:genetics; D051379:Mice; D008807:Mice, Inbred BALB C; D012160:Retina / Q000473:pathology / Q000528:radiation effects; D012162:Retinal Degeneration / Q000235:genetics*