Rapid, convenient radioimmunoassay of estrone sulfate.
We developed a specific, simple, and rapid RIA for the direct quantification of estrone sulfate (E1S) and established its performance characteristics. The assay has a dynamic range of 0.05-90 micrograms/L with a detection limit of 0.009 microgram/L. Intraassay CVs were 9.2%, 4.5%, and 4.6% at 0.35, 9.0, and 60 micrograms/L, respectively. Interassay CVs were 8.8%, 5.1%, and 5.5% at 0.076, 0.5, and 12 micrograms/L, respectively. Linearity of dilution studies showed values of 80-105% of expected, and recovery of E1S added to serum samples ranged from 82% to 102%. Cross-reactivities with structurally related estrogens were < 5%. When compared with a conventional assay (involving hydrolysis of E1S and indirect measurement of estrone), the present RIA showed excellent correlation (r = 0.99, slope = 1.54, Sy/x = 2.14, n = 71). Mean E1S concentrations measured with this RIA for normal men (n = 20) and women in follicular (n = 20) and luteal (n = 25) phases of their menstrual cycle were 0.96, 0.96, and 1.74 microgram/L, respectively. Mean E1S concentrations for oral contraceptive users (n = 20) and postmenopausal women without hormone replacement therapy (n = 21) or on hormone replacement therapy (n = 22) were 0.74, 0.13, and 2.56 micrograms/L, respectively. Serum concentrations of E1S in pregnant women in their first (n = 14), second (n = 17), and third (n = 15) trimesters were 20, 66, and 105 micrograms/L, respectively. Availability of this simple RIA should provide a useful tool for the assessment of estrogen status in women.
Ranadive
G N
GN
Diagnostic Systems Laboratories, Webster, TX 77598-4217, USA.
Mistry
J S
JS
Damodaran
K
K
Khosravi
M J
MJ
Diamandi
A
A
Gimpel
T
T
Castracane
V D
VD
Patel
S
S
Stanczyk
F Z
FZ
D003429:Cross Reactions; D004970:Estrone / Q000031:analogs & derivatives* / Q000097:blood; D005260:Female; D006801:Humans; D008297:Male; D008597:Menstrual Cycle / Q000502:physiology; D015394:Molecular Structure; D017698:Postmenopause / Q000097:blood; D011247:Pregnancy; D011863:Radioimmunoassay / Q000379:methods*; D012680:Sensitivity and Specificity
D004970:Estrone; C017296:estrone sulfate