title: [Exceptional production of leukocyte-free erythrocyte concentrates using filtration with the BPF 4 BBS leukocyte filter].
abstract: Leukocyte depletion prevents some undesirable effects of red cell transfusions. We evaluated the blood filter system BPF 4 BBS (PALL) by filtering 26 units of buffy coat-free red cell concentrates, prepared from 500 ml of whole blood and suspended in SAG-M-additive solution, over 8 min within 48 h after donation at room temperature. Filtration reduced the leukocyte content by more than 99.99% in 25 of the 26 units examined. In 20 instances, no leukocyte was found in the Nageotte counting chamber (2 x 50 microliters) corresponding to a total leukocyte count below 2.38 x 10(4). In 5 instances a single leukocyte was seen, corresponding to a total leukocyte count of (2.35 +/- 0.01) x 10(4). One unit contained 2.93 x 10(5) leukocytes after filtration, corresponding to a 99.98% leukocyte reduction. Red cell recovery was 92.5 +/- 3.8%. This system is well suited for routine use.
authors.affiliation: Bluttransfusionsdienst, Zentralkrankenhaus St.-Jürgen-Strasse, Bremen, Deutschland.
authors.forename: B
authors.initials: B
authors.lastname: Zimmermann
authors.forename: I
authors.initials: I
authors.lastname: Hillringhaus
authors.forename: U
authors.initials: U
authors.lastname: Diekamp
mesh_terms: D016238:Cytapheresis / Q000295:instrumentation* / Q000379:methods; D017707:Erythrocyte Transfusion; D004912:Erythrocytes; D005374:Filtration / Q000295:instrumentation / Q000379:methods; D006801:Humans; D007962:Leukocytes
vernacular_title: Das Herstellen weitgehend leukozytenfreier Erythrozytenkonzentrate durch Filtern mit dem Leukozytenfilter BPF 4 BBS.