title: The cellular response of lambs to Brucella ovis before and after birth.
abstract: The immunological response of lambs to Brucella ovis before and after birth was investigated. The establishment of indwelling cannulas in the efferent prescapular lymphatic ducts of foetal lambs allowed continual monitoring of the immune response of a single lymph node. Foetal lambs in the last trimester of pregnancy were shown to mount a strong cell-mediated immune response to B. ovis. Lymphocytes from the challenged lymph node stimulated with B. ovis in vitro usually first reacted significantly and had highest [3H]-thymidine incorporation between 4 and 6 days after primary and secondary challenge, whereas, lymphocytes from the unchallenged node did not exhibit significant [3H]-thymidine incorporation until some 24 h later. Lymphocytes from these lambs challenged as foetuses still exhibited significant [3H]-thymidine incorporation in response to B. ovis for 4 to 5 months after birth. The proportion of surface immunoglobulin-positive cells in efferent prescapular lymph of unchallenged lambs ranged from 0.5 to 2.0% but after B. ovis challenge this proportion ranged from 2.7 to 8.7% between 4 to 6 days after challenge. By 9 to 12 days after challenge, the proportion had declined to pre-challenge values.
authors.affiliation: John Curtin School of Medical Research, Australian National University, Canberra, Australia. tierneyt@dpi.qld.gov.au
authors.forename: T J
authors.initials: TJ
authors.lastname: Tierney
authors.forename: W
authors.initials: W
authors.lastname: Trevella
authors.forename: M W
authors.initials: MW
authors.lastname: Simpson-Morgan
mesh_terms: D000818:Animals; D002006:Brucellosis / Q000276:immunology / Q000662:veterinary*; D005333:Fetus / Q000276:immunology; D008213:Lymphocyte Activation; D012756:Sheep; D012757:Sheep Diseases / Q000276:immunology*