title: Neutrophil-activating protein mediates adhesion of Helicobacter pylori to sulfated carbohydrates on high-molecular-weight salivary mucin.
abstract: The in vitro binding of surface-exposed material and outer membrane proteins of Helicobacter pylori to high-molecular-weight salivary mucin was studied. We identified a 16-kDa surface protein which adhered to high-molecular-weight salivary mucin. This protein binds specifically to sulfated oligosaccharide structures such as sulfo-Lewis a, sulfogalactose and sulfo-N-acetyl-glucosamine on mucin. Sequence analysis of the protein proved that it was identical to the N-terminal amino acid sequence of neutrophil-activating protein. Moreover, this adhesin was able to bind to Lewis x blood group antigen.
authors.affiliation: Department of Medical Microbiology, Medical School, Vrije Universiteit, Amsterdam, The Netherlands. F.Namavar.mm@med.vu.nl
authors.forename: F
authors.initials: F
authors.lastname: Namavar
authors.forename: M
authors.initials: M
authors.lastname: Sparrius
authors.forename: E C
authors.initials: EC
authors.lastname: Veerman
authors.forename: B J
authors.initials: BJ
authors.lastname: Appelmelk
authors.forename: C M
authors.initials: CM
authors.lastname: Vandenbroucke-Grauls
mesh_terms: D000595:Amino Acid Sequence; D001422:Bacterial Adhesion; D001426:Bacterial Proteins / Q000502:physiology*; D018821:CD18 Antigens / Q000502:physiology; D016480:Helicobacter pylori / Q000502:physiology*; D006801:Humans; D007983:Lewis Blood Group Antigens / Q000378:metabolism; D008969:Molecular Sequence Data; D008970:Molecular Weight; D009077:Mucins / Q000378:metabolism*; D009844:Oligosaccharides / Q000378:metabolism; D012463:Saliva / Q000382:microbiology*
chemical_list: D001426:Bacterial Proteins; D018821:CD18 Antigens; D007983:Lewis Blood Group Antigens; D009077:Mucins; D009844:Oligosaccharides; C092412:neutrophil-activating protein A, Helicobacter pylori