Establishment of a new human myeloma cell line, KMS-18, having t(4;14)(p16.3;q32.3) derived from a case phenotypically transformed from Ig A-lambda to BJP-lambda, and associated with hyperammonemia.
A new human myeloma cell line, KMS-18, was established from a 58-year-old male with multiple myeloma associated with hyperammonemia. The original leukemic cells and established KMS-18 cells possessed several of the same chromosomal abnormalities, including add(1)(q32), add(10) (q24) and add(17)(p11). In addition, the KMS-18 cells showed novel t(4;14)(p16.3;q32.3) masked translocation which was determined by the FISH method. Moreover, we compared the ammonia production in culture medium of the KMS-18 cell line with that of non-myeloma hematological malignant cell lines and a hepatocellular carcinoma cell line. KMS-18 produced higher levels of ammonia in medium than the other cell lines examined. This new cell line may prove helpful in analyzing the role and biological mechanisms of the t(4;14)(p16.3;q32.3) translocation in myeloma and also in investigating hyperammonemia in cases with myeloma.
Otsuki
T
T
Department of Hygiene, Kawasaki Medical School, 577 Matsushima, Kurashiki, Okayama, 701-01, Japan.
Nakazawa
N
N
Taniwaki
M
M
Yamada
O
O
Sakaguchi
H
H
Wada
H
H
Yawata
Y
Y
Ueki
A
A
D000641:Ammonia / Q000097:blood* / Q000378:metabolism; D015703:Antigens, CD / Q000032:analysis; D006528:Carcinoma, Hepatocellular / Q000378:metabolism / Q000473:pathology; D018929:Cell Culture Techniques / Q000379:methods; D002874:Chromosome Mapping; D002883:Chromosomes, Human, Pair 14; D002894:Chromosomes, Human, Pair 4; D019337:Hematologic Neoplasms / Q000378:metabolism / Q000473:pathology; D004854:Herpesvirus 4, Human / Q000302:isolation & purification; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D007621:Karyotyping; D008113:Liver Neoplasms / Q000378:metabolism / Q000473:pathology; D008297:Male; D008875:Middle Aged; D009101:Multiple Myeloma / Q000235:genetics* / Q000276:immunology / Q000378:metabolism / Q000473:pathology*; D009174:Mycoplasma / Q000302:isolation & purification; D010641:Phenotype; D016133:Polymerase Chain Reaction; D014178:Translocation, Genetic; D014407:Tumor Cells, Cultured
D015703:Antigens, CD; D000641:Ammonia