Differences in the active site environment of Aspergillus ficuum phytases.
While Aspergillus ficuum phytaseA (phyA) was rapidly inactivated by 1,2-cyclohexanedione and phenylglyoxal, both specific modifiers of arginine, phytaseB (phyB) showed a markedly different behavior. First, phyB was totally insensitive to 1,2-cyclohexanedione even in the presence of 0.2 M guanidinium hydrochloride; second, the enzyme showed a great deal of resistance to inactivation by phenylglyoxal. Taken together, these results indicate that the chemical environment of the active site of phyB is very different from that of the active site of phyA. Despite sequence similarities of the active site region in these two proteins, their differential behavior to arginine modifiers indicates that other parts of the protein play a role in the active site formation. We expected some differences in the structure since the proteins have dissimilar kinetic parameters and pH optima.
Ullah
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Southern Regional Research Center, ARS, USDA, New Orleans, Louisiana 70124, USA.
Sethumadhavan
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D010832:6-Phytase / Q000037:antagonists & inhibitors / Q000737:chemistry* / Q000378:metabolism; D001120:Arginine / Q000378:metabolism; D001230:Aspergillus / Q000201:enzymology*; D001665:Binding Sites / Q000235:genetics; D003512:Cyclohexanones / Q000494:pharmacology; D004791:Enzyme Inhibitors / Q000494:pharmacology; D005656:Fungal Proteins / Q000737:chemistry / Q000378:metabolism; D019791:Guanidine / Q000494:pharmacology; D006863:Hydrogen-Ion Concentration; D007527:Isoenzymes / Q000037:antagonists & inhibitors / Q000378:metabolism; D007700:Kinetics; D010658:Phenylglyoxal / Q000494:pharmacology
D003512:Cyclohexanones; D004791:Enzyme Inhibitors; D005656:Fungal Proteins; D007527:Isoenzymes; C018031:1,2-cyclohexanedione; D001120:Arginine; D010832:6-Phytase; D019791:Guanidine; D010658:Phenylglyoxal