Expression of HMGI(Y) proteins in squamous intraepithelial and invasive lesions of the uterine cervix.
The expression of nuclear proteins high mobility group (HMG) I and HMGY was investigated in intraepithelial and invasive lesions of the uterine cervix. Human carcinoma cell lines C-41, ME-180, and CaSki were used for testing protein expression in neoplastic cells from the cervix. Morphological grading of the dysplasias (CIN 1, CIN 2, and CIN 3) and invasive carcinomas from formalin-fixed paraffin-embedded samples parallels the degree of nuclear immunostaining obtained using a polyclonal antibody raised against the amino-terminal region of HMGI(Y) proteins. The immunostaining obtained with HMGI(Y) antibody was compared with that observed using the antibody Ki-67, and the results were similar. We suggest the use of HMGI(Y) antibody in clinical oncology as a useful marker of intraepithelial lesions and invasive carcinomas.
Bandiera
A
A
Dipartimento di Biochimica, Biofisica e Chimica delle Macromolecole, Universitá degli Studi di Trieste, Italy.
Bonifacio
D
D
Manfioletti
G
G
Mantovani
F
F
Rustighi
A
A
Zanconati
F
F
Fusco
A
A
Di Bonito
L
L
Giancotti
V
V
D000911:Antibodies, Monoclonal / Q000276:immunology; D000951:Antigens, Neoplasm / Q000032:analysis*; D014408:Biomarkers, Tumor / Q000032:analysis*; D015152:Blotting, Northern; D015153:Blotting, Western; D002294:Carcinoma, Squamous Cell / Q000737:chemistry* / Q000175:diagnosis / Q000473:pathology; D005260:Female; D015870:Gene Expression; D025741:HMGA1a Protein; D006609:High Mobility Group Proteins / Q000032:analysis* / Q000276:immunology; D006801:Humans; D007124:Immunoenzyme Techniques; D019394:Ki-67 Antigen / Q000032:analysis; D009361:Neoplasm Invasiveness; D016612:Paraffin Embedding; D014407:Tumor Cells, Cultured; D002583:Uterine Cervical Neoplasms / Q000737:chemistry* / Q000175:diagnosis / Q000473:pathology; D002578:Uterine Cervical Dysplasia / Q000737:chemistry* / Q000175:diagnosis / Q000473:pathology
D000911:Antibodies, Monoclonal; D000951:Antigens, Neoplasm; D014408:Biomarkers, Tumor; D006609:High Mobility Group Proteins; D019394:Ki-67 Antigen; D025741:HMGA1a Protein