[Neutralizing human antibody specific for human cytomegalovirus in E. coli expression system].
We isolated neutralizing human Fab fragment specific for human cytomegalovirus (HCMV) by phage display system. Fab libraries were constructed from peripheral lymphocyte of healthy individual. In several clones reacted for HCMV-infected HEL cells, one clone, designated 13-3, stained HCMV infected cells at 96 hrs post infection. It didn't react to cells at 6 hrs post infection or infected cells in the presence of AraC, meaning that 13-3 recognized protein synthesized at late times of infection. It also neutralized HCMV, Towne strain, efficiently. This neutralizing activity was specific for HCMV and no effect for HSV-1 and 2.
Ihara
S
S
Department of Molecular Life Science 2, Tokai University, School of Medicine.
Maeda
F
F
Takekoshi
M
M
D000818:Animals; D000911:Antibodies, Monoclonal; D000918:Antibody Specificity; D001435:Bacteriophages; D001483:Base Sequence; D003001:Cloning, Molecular / Q000379:methods*; D003587:Cytomegalovirus / Q000276:immunology*; D017931:DNA Primers; D004926:Escherichia coli; D006801:Humans; D007140:Immunoglobulin Fab Fragments / Q000302:isolation & purification*; D008969:Molecular Sequence Data
D000911:Antibodies, Monoclonal; D017931:DNA Primers; D007140:Immunoglobulin Fab Fragments