title: Lectin histochemical studies of mouse granulated metrial gland cells.
abstract: A lectin histochemical study has been carried out on mouse granulated metrial gland cells, the major leucocyte population that differentiates in the uterine wall in pregnancy. The binding characteristics of 26 lectins were examined using light microscopical methods. Fourteen of the lectins, with affinities ranging through N-acetylgalactosamine, galactose, N-acetylglucosamine, mannose and sialic acid residues, bound to the cytoplasmic granules of granulated metrial gland cells, and each appeared to bind to the limiting membrane of the granules. The binding characteristics of three of these lectins (Wheat germ agglutinin, Concanavalin A and Helix pomatia agglutinin) were examined using electron microscopical methods. These showed a different binding pattern to the cytoplasmic granules of granulated metrial gland cells compared with that found using light microscopical methods, as they appeared to bind evenly across the granule's matrix. This binding pattern corresponds to the reactivity of the granule matrix in the periodic acid-Schiff technique. Six lectins bound to the cell membranes of granulated metrial gland cells. These included the E and L isoforms of Phaseolus vulgaris agglutinin, with affinities for complex carbohydrates, whose binding differences were related to the stage of differentiation of the granulated metrial gland cells. The lectin binding described presents additional markers of granulated metrial gland cells and tools for investigating carbohydrate moieties in the functional activities of granulated metrial gland cells.
authors.affiliation: School of Medicine, University of Southampton, Bassett Crescent East, UK.
authors.forename: I J
authors.initials: IJ
authors.lastname: Stewart
authors.forename: A J
authors.initials: AJ
authors.lastname: Webster
mesh_terms: D000818:Animals; D050260:Carbohydrate Metabolism; D002241:Carbohydrates / Q000032:analysis*; D002462:Cell Membrane / Q000737:chemistry / Q000378:metabolism; D003594:Cytoplasmic Granules / Q000737:chemistry / Q000378:metabolism; D005260:Female; D037102:Lectins / Q000378:metabolism*; D008791:Metrial Gland / Q000737:chemistry* / Q000166:cytology* / Q000648:ultrastructure; D051379:Mice; D008854:Microscopy, Electron; D010503:Periodic Acid-Schiff Reaction; D011247:Pregnancy; D011485:Protein Binding; D012680:Sensitivity and Specificity
chemical_list: D002241:Carbohydrates; D037102:Lectins