Serological response of patients infected with Salmonella typhi.
OBJECTIVE
To evaluate a rapid immunoblotting procedure for providing evidence of infection with Salmonella typhi using 73 sera from patients infected with S typhi.


METHODS
A sodium dodecyl sulphatepolyacrylamide gel electrophoresis (SDS-PAGE)/immunoblotting procedure using lipopolysaccharide (LPS, O = 9,12) and flagellar (H = d) antigens was used.


RESULTS
Seventy two of 73 sera contained antibodies to LPS, 40 sera also contained antibodies to H = d flagellar antigens. Analysis of acute and convalescent sera showed that only 62% of patients produced antibodies to flagellar antigens.


CONCLUSIONS
The SDS-PAGE/immunoblotting procedure provided a rapid method for providing serological evidence of infection with S typhi.
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Laboratory of Enteric Pathogens, Central Public Health Laboratory, London, UK.
Rowe
B
B
Cheesbrough
J S
JS
D000208:Acute Disease; D000293:Adolescent; D000328:Adult; D000907:Antibodies, Bacterial / Q000097:blood*; D000942:Antigens, Bacterial / Q000276:immunology; D015415:Biomarkers / Q000097:blood; D004591:Electrophoresis, Polyacrylamide Gel; D005069:Evaluation Studies as Topic; D005260:Female; D005407:Flagella / Q000276:immunology; D006801:Humans; D015151:Immunoblotting; D008070:Lipopolysaccharides / Q000276:immunology; D008297:Male; D008875:Middle Aged; D012477:Salmonella enteritidis / Q000276:immunology; D012485:Salmonella typhi / Q000276:immunology*; D014435:Typhoid Fever / Q000175:diagnosis* / Q000276:immunology
D000907:Antibodies, Bacterial; D000942:Antigens, Bacterial; D015415:Biomarkers; D008070:Lipopolysaccharides