Priming response to inflammatory mediators in hyperreactive peripheral neutrophils from adult periodontitis.
OBJECTIVE
To study the response to in vitro priming of peripheral neutrophils from patients with periodontitis compared to healthy controls. Peripheral neutrophils from these patients had shown increased production of oxygen radicals after activation with opsonized bacteria and a difference in priming response could suggest an explanation for this hyperreactivity.


METHODS
Peripheral neutrophils from a group of patients with periodontitis and from age- and sex-matched controls were preincubated with tumor necrosis factor alpha, lipopolysaccharide (LPS), formyl-methionyl-leucyl-phenylalanine and the tetra peptide arginyl-glycyl-aspartate-serine. After preincubation, the cells were activated with gammaglobulin opsonized-bacteria, i.e., a Fc gamma R-stimulation. The priming effect was assessed as the production of oxygen radicals and as the degranulation or primary granules.


RESULTS
Showed that the patients had a slightly lower responsiveness to priming than had the controls. This difference in priming response was most pronounced when measured as degranulation of primary granules after preincubation with LPS and 20 min of activation.


CONCLUSIONS
This study shows no difference in response to priming, with optimal concentrations of inflammatory mediators, between peripheral neutrophils from patients with adult periodontitis and healthy controls.
Gustafsson
A
A
Division of Periodontology, Karolinska Institute, Huddinge, Sweden.
Asman
B
B
Bergström
K
K
D000328:Adult; D016022:Case-Control Studies; D015550:Cell Degranulation; D005260:Female; D005609:Free Radicals / Q000378:metabolism; D006801:Humans; D018836:Inflammation Mediators / Q000494:pharmacology* / Q000502:physiology; D019272:Leukocyte Elastase / Q000096:biosynthesis; D008070:Lipopolysaccharides / Q000494:pharmacology; D008297:Male; D008875:Middle Aged; D009240:N-Formylmethionine Leucyl-Phenylalanine / Q000494:pharmacology; D018375:Neutrophil Activation / Q000502:physiology; D009504:Neutrophils / Q000187:drug effects / Q000502:physiology*; D009842:Oligopeptides / Q000494:pharmacology; D010518:Periodontitis / Q000097:blood / Q000276:immunology*; D017382:Reactive Oxygen Species / Q000378:metabolism*; D017452:Receptors, IgG / Q000378:metabolism; D016897:Respiratory Burst; D014409:Tumor Necrosis Factor-alpha / Q000494:pharmacology
D005609:Free Radicals; D018836:Inflammation Mediators; D008070:Lipopolysaccharides; D009842:Oligopeptides; D017382:Reactive Oxygen Species; D017452:Receptors, IgG; D014409:Tumor Necrosis Factor-alpha; D009240:N-Formylmethionine Leucyl-Phenylalanine; C046617:arginyl-glycyl-aspartyl-serine; D019272:Leukocyte Elastase