Endothelial cell conditioned media mediated regulation of glutamine synthetase activity in glial cells.
The responsiveness of late passage C-6 glial cells to human retinal endothelial cell-conditioned medium (HREC-CM) was examined using glutamine synthetase (GS) activity as test parameter. Treatment with 50% or 100% HREC-CM for 4-5 days slightly affected the morphology but significantly increased GS activity. Increased glial GS activity induced by vascular endothelial cells is of relevance in preventing extracellular glutamate toxicity and regulating the brain/retinal blood barrier.
Spoerri
P E
PE
Department of Medicine, University of Florida, Health Sciences Center, Gainesville 32610, USA.
Grant
M B
MB
Gomez
J
J
Vernadakis
A
A
D001253:Astrocytes / Q000201:enzymology; D015415:Biomarkers / Q000737:chemistry; D017077:Culture Media, Conditioned; D004730:Endothelium, Vascular / Q000166:cytology*; D005974:Glutamate-Ammonia Ligase / Q000378:metabolism*; D006801:Humans; D009457:Neuroglia / Q000201:enzymology*; D012171:Retinal Vessels / Q000166:cytology*; D014407:Tumor Cells, Cultured
D015415:Biomarkers; D017077:Culture Media, Conditioned; D005974:Glutamate-Ammonia Ligase