Evaluation of single cell sources of docosahexaenoic acid and arachidonic acid: a 4-week oral safety study in rats.
Docosahexaenoic acid (DHA) and arachidonic acid (ARA) are secreted in human milk and consumed by the nursing neonate but are not present in infant formulas currently available in the US. Supplementation of formulas with DHA and ARA may be particularly important for premature infants, who have less accretion of these fatty acids in utero than term infants. Some experts suggest that DHA and ARA should be added to infant formulas. Common sources of these fatty acids (e.g. fish oils, egg yolk lipids) are not optimal for infants in that they contain disproportionate amounts of other fatty acids. This 4-wk study examined the safety of a high-DHA algal oil and a high-ARA fungal oil, blended so that the DHA:ARA ratio approximates that in human milk. Rats were fed the blend at levels representing three, 11 and 22 times the anticipated infant exposure. Control animals were fed either a high-fat diet (13.1%, w/w; equivalent to the fat content of the treated groups) or a low-fat diet (5%, w/w). There were no treatment-related differences in body weight, food intake, organ weights, haematology or clinical chemistry. Thus, this study indicates that a blend of algal and fungal oils is a safe source of DHA and ARA as it produced no adverse effects in rats when administered for 4 wk at levels up to 22 times the expected infant exposure.
Wibert
G J
GJ
Mead Johnson Research Center, Evansville, IN 47721, USA.
Burns
R A
RA
Diersen-Schade
D A
DA
Kelly
C M
CM
D000284:Administration, Oral; D000818:Animals; D016718:Arachidonic Acid / Q000302:isolation & purification / Q000633:toxicity*; D001835:Body Weight / Q000187:drug effects; D004141:Dinoflagellida / Q000737:chemistry*; D004281:Docosahexaenoic Acids / Q000302:isolation & purification / Q000633:toxicity*; D004338:Drug Combinations; D004435:Eating / Q000187:drug effects; D005260:Female; D008099:Liver / Q000187:drug effects / Q000473:pathology; D008297:Male; D009090:Mucorales / Q000737:chemistry*; D009504:Neutrophils / Q000187:drug effects / Q000473:pathology; D009929:Organ Size / Q000187:drug effects; D051381:Rats; D017207:Rats, Sprague-Dawley; D012449:Safety; D015996:Survival Rate
D004338:Drug Combinations; D004281:Docosahexaenoic Acids; D016718:Arachidonic Acid