Direct structural analysis of 2-aminoacridone derivatized oligosaccharides by high-performance liquid chromatography/mass spectrometric detection.
In this preliminary study linear and branched oligosaccharides, derivatized with 2-aminoacridone, have been separated by reverse-phase high-performance liquid chromatography, and detected by positive-ion electrospray mass spectrometry. Tandem Mass spectra of the protonated carbohydrate analytes gave simple fragmentation patterns from which sequence information could be readily obtained.
Camilleri
P
P
SmithKline Beecham Pharmaceuticals, Harlow, Essex, UK.
Tolson
D
D
Birrell
H
H
D000609:Aminoacridines / Q000737:chemistry*; D002240:Carbohydrate Sequence; D002241:Carbohydrates / Q000032:analysis; D002851:Chromatography, High Pressure Liquid; D003911:Dextrans; D005456:Fluorescent Dyes / Q000737:chemistry*; D008401:Gas Chromatography-Mass Spectrometry; D013058:Mass Spectrometry; D008969:Molecular Sequence Data; D009844:Oligosaccharides / Q000737:chemistry*; D013056:Spectrophotometry, Ultraviolet
D000609:Aminoacridines; D002241:Carbohydrates; D003911:Dextrans; D005456:Fluorescent Dyes; D009844:Oligosaccharides; C072515:2-aminoacridone