Differentiation-specific nuclear matrix proteins cross-linked to DNA by cis-diammine dichloroplatinum.
DNA-protein cross-linkages were performed in intact undifferentiated and differentiated-HL60 cells by the action of cis-diammine dichloroplatinum. Total nuclear matrix proteins and DNA cross-linked nuclear matrix proteins were resolved by two-dimensional gel electrophoresis. The comparison of the electrophoretic patterns allowed the identification of a set of differentiation-induced nuclear matrix proteins cross-linked to DNA. One of these proteins binds cloned histone SAR sequences. Our results outline an experimental strategy for isolating and characterizing nuclear matrix components that may play a fundamental role in the overall control and coordination of gene expression during differentiation.
Mattia
E
E
Microbiology Institute Faculty of Medicine and Surgery, University of Rome La Sapienza, Italy. Mattia@axrma.uniroma1.it
Eufemi
M
M
Chichiarelli
S
S
Ceridono
M
M
Ferraro
A
A
D034961:Antigens, Nuclear; D002454:Cell Differentiation; D002945:Cisplatin / Q000493:pharmacokinetics* / Q000494:pharmacology; D003432:Cross-Linking Reagents; D015180:Electrophoresis, Gel, Two-Dimensional; D018922:HL-60 Cells / Q000166:cytology*; D006801:Humans; D015151:Immunoblotting; D008970:Molecular Weight; D009687:Nuclear Proteins / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism*
D034961:Antigens, Nuclear; D003432:Cross-Linking Reagents; D009687:Nuclear Proteins; D002945:Cisplatin