Fate of adult neural precursors grafted to adult cortex monitored with a Y-chromosome marker.
Freshly dissected and dissociated subventricular zone cells from adult male mice were transplanted into the right cerebral cortex of adult female mice. Host mice were perfused 3 or 7 weeks later. To detect donor male cells, paraffin sections (10 microm) were hybridized in situ with a Y-chromosome-specific probe. Some hybridized sections were also immunostained with antibodies against glial fibrillary acidic protein (GFAP), NeuN or beta-tubulin. Grafted cells were identified in 80% of host female brains; they were found in both grey and white matter and were located close to the initial injection site. Double-labelling studies showed that, of the surviving cells containing hybridized male nuclei, 5-10% expressed a glial (GFAP) marker and 6-7% expressed neuronal (NeuN or beta-tubulin) markers.
Harvey
A R
AR
Department of Anatomy and Human Biology, University of Western Australia, Nedlands, Perth.
Symons
N A
NA
Pollett
M A
MA
Brooker
G J
GJ
Bartlett
P F
PF
D000818:Animals; D002540:Cerebral Cortex / Q000637:transplantation*; D005260:Female; D005819:Genetic Markers; D007150:Immunohistochemistry; D017403:In Situ Hybridization; D066298:In Vitro Techniques; D008297:Male; D051379:Mice; D008808:Mice, Inbred CBA; D009474:Neurons / Q000637:transplantation*; D016612:Paraffin Embedding; D010641:Phenotype; D014998:Y Chromosome
D005819:Genetic Markers