Quantitative determinations of the steady state transcript levels of hexokinase isozymes and glucose transporter isoforms in normal rat tissues and the malignant tumor cell line AH130.
The steady state transcript levels of the four hexokinase (HK) isozymes and four glucose transporter (GLUT) isoforms were determined quantitatively by Northern analysis of RNA samples from rat tissues using synthetic fragments of the RNAs encoding the HK isozymes and GLUT isoforms. Results showed that the levels of HK isozyme transcripts were low in rat tissues, the level of that most highly expressed, the type I isozyme (HKI), in the brain being 0.025% of the total poly(A)+ RNA. A good correlation was found between the reported HK activities and the total amounts of transcripts encoding all HK isozymes in various tissues, showing that the HK activities in tissues can be estimated from the total amount of transcripts encoding HK isozymes. The proposed associated expressions of HK isozymes and GLUT isoforms in particular tissues were confirmed at their transcript levels. The steady state transcript levels of type II HK and the type 1 GLUT isoform in the malignant tumor cell line AH130 were also determined quantitatively.
Shinohara
Y
Y
Faculty of Pharmaceutical Sciences, University of Tokushima, Japan. yasuo@ph.tokushima-u.ac.jp
Yamamoto
K
K
Inoo
K
K
Yamazaki
N
N
Terada
H
H
D000818:Animals; D004587:Electrophoresis, Agar Gel; D006593:Hexokinase / Q000235:genetics*; D007527:Isoenzymes / Q000235:genetics*; D009004:Monosaccharide Transport Proteins / Q000378:metabolism*; D012333:RNA, Messenger / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D051381:Rats; D014407:Tumor Cells, Cultured
D007527:Isoenzymes; D009004:Monosaccharide Transport Proteins; D012333:RNA, Messenger; D006593:Hexokinase