title: Analysis of cell kinetics after irradiation by flow cytometry: proliferative ability of G2-blocked cells after cell sorting.
abstract: Irradiation, dose-dependently, increases the percentage of cells in the G2 + M phase and the duration of cell cycle arrest, reflected by changes in cell kinetics in the first 48 h. Cells in the G2 + M phase are considered radiosensitive but little is known about their proliferative ability. We studied proliferative ability of irradiated G2 phase-arrested cells following flow cytometric cell sorting using fluorescent Hoechst 33342 staining. Although proliferative ability of non-irradiated cells was not different between G1 and G2 + M phases, cells arrested in G2 + M after irradiation, especially at higher doses, showed less ability than cells in G1. Proliferative ability also correlated well with of G2 block duration.
authors.affiliation: Department of Radiology, Kansai Medical University, 10-15 Fumizonochou, Moriguchi City, Osaka, 570, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Murata
authors.forename: K
authors.initials: K
authors.lastname: Akagi
authors.forename: H
authors.initials: H
authors.lastname: Kimura
authors.forename: R
authors.initials: R
authors.lastname: Nasu
authors.forename: M
authors.initials: M
authors.lastname: Imamura
authors.forename: Y
authors.initials: Y
authors.lastname: Tanaka
mesh_terms: D001562:Benzimidazoles / Q000633:toxicity; D002455:Cell Division / Q000502:physiology* / Q000528:radiation effects; D002470:Cell Survival / Q000528:radiation effects; D004273:DNA, Neoplasm / Q000032:analysis; D005434:Flow Cytometry; D005456:Fluorescent Dyes / Q000633:toxicity; D016193:G1 Phase / Q000502:physiology / Q000528:radiation effects; D016195:G2 Phase / Q000502:physiology* / Q000528:radiation effects; D005720:Gamma Rays; D006367:HeLa Cells / Q000187:drug effects / Q000528:radiation effects; D006801:Humans; D007700:Kinetics; D008938:Mitosis / Q000502:physiology / Q000528:radiation effects; D011003:Ploidies
chemical_list: D001562:Benzimidazoles; D004273:DNA, Neoplasm; D005456:Fluorescent Dyes; C017807:bisbenzimide ethoxide trihydrochloride