title: Different growth properties in response to epidermal growth factor and interleukin-6 of primary keratinocytes derived from normal and psoriatic lesional skin.
abstract: Epidermal growth factor (EGF) family members and its receptor (EGFR) are thought to have an important role in the proliferation of epidermal keratinocytes. In this report, we investigated the EGF/EGFR system in primary keratinocytes derived from normal and psoriatic lesional skin. EGF elicited the growth of both normal human keratinocytes (NHKs) and psoriatic lesional keratinocytes (PLKs). Interleukin-6 (IL-6) potentiated the EGF-dependent growth of NHKs, but has no observable effect on PLKs, while IL-6 itself showed no growth-stimulating activities in both cell types. Immunodetection and in situ hybridization analyses revealed that IL-6 induces EGFR expression in NHKs in a time- and dose-dependent manner. This EGFR expression decreased reversibly to an undetectable level when IL-6-treated NHKs were re-cultured in IL-6-free conditions. On the other hand, PLKs expressed high levels of EGFR even when unstimulated and the expression level was not affected by IL-6 stimulation. These results suggest that the EGF/EGFR system is involved in the growth of NHKs and PLKs and that IL-6 potentiates NHK growth partly through the induction of EGFR. The different EGFR regulatory system may contribute to the pathogenesis of psoriasis.
authors.affiliation: Department of Dermatology, Fukushima Medical College, Japan.
authors.forename: N
authors.initials: N
authors.lastname: Oyama
authors.forename: M
authors.initials: M
authors.lastname: Sekimata
authors.forename: Y
authors.initials: Y
authors.lastname: Nihei
authors.forename: K
authors.initials: K
authors.lastname: Iwatsuki
authors.forename: Y
authors.initials: Y
authors.lastname: Homma
authors.forename: F
authors.initials: F
authors.lastname: Kaneko
mesh_terms: D000328:Adult; D002455:Cell Division / Q000187:drug effects / Q000502:physiology; D004815:Epidermal Growth Factor / Q000494:pharmacology*; D066246:ErbB Receptors / Q000187:drug effects / Q000235:genetics; D015870:Gene Expression / Q000235:genetics; D005786:Gene Expression Regulation; D006801:Humans; D015850:Interleukin-6 / Q000494:pharmacology*; D015603:Keratinocytes / Q000187:drug effects* / Q000502:physiology; D008875:Middle Aged; D011565:Psoriasis / Q000473:pathology* / Q000503:physiopathology; D012867:Skin / Q000737:chemistry / Q000187:drug effects* / Q000473:pathology
chemical_list: D015850:Interleukin-6; D004815:Epidermal Growth Factor; D066246:ErbB Receptors