title: Enhanced withdrawal responses to mechanical and thermal stimuli after bone injury.
abstract: Withdrawal responses to mechanical and thermal stimuli applied to the plantar surface of the hindpaw were measured before and after bone damage. In separate groups of rats the bone was injured by scraping the periosteum of the tibia, drilling a hole through the tibia, aspirating bone marrow, or drilling a hole through the calcaneus. Scraping the periosteum did not alter withdrawal responses to the mechanical stimuli, or evoke nocifensive behavior. In contrast, secondary mechanical hyperalgesia and allodynia, and cold allodynia were observed after a hole was drilled through the tibia or calcaneus and after aspiration of bone marrow. The secondary hyperalgesia peaked at 2 h after injury. Drilling a hole through the calcaneus permitted primary hyperalgesia to be easily quantified. Primary hyperalgesia lasted up to 24 h after injury. Nocifensive behavior characterized by a lifting and guarding of the damaged limb was also observed after a hole was drilled through the tibia or calcaneus. Drilling a hole through the tibia or calcaneus should be a useful experimental model for investigating the mechanisms underlying bone pain.
authors.affiliation: Marine Biomedical Institute, University of Texas Medical Branch, 301 University Boulevard, Galveston, TX 77555-1069, USA 1002 East South Temple, Salt Lake City, UT 84102, USA.
authors.forename: Andrea K
authors.initials: AK
authors.lastname: Houghton
authors.forename: Elena
authors.initials: E
authors.lastname: Hewitt
authors.forename: Karin N
authors.initials: KN
authors.lastname: Westlund
mesh_terms: D000704:Analysis of Variance; D000818:Animals; D001853:Bone Marrow / Q000293:injuries; D001842:Bone and Bones / Q000293:injuries*; D002111:Calcaneus / Q000293:injuries; D003080:Cold Temperature; D006358:Hot Temperature; D008297:Male; D010146:Pain / Q000503:physiopathology*; D010521:Periosteum / Q000293:injuries; D051381:Rats; D017207:Rats, Sprague-Dawley; D012018:Reflex / Q000502:physiology*; D015203:Reproducibility of Results; D017695:Soft Tissue Injuries / Q000503:physiopathology; D013314:Stress, Mechanical; D013977:Tibia / Q000293:injuries