Detachment of linking film bacteria from enamel surfaces by oral rinses and penetration of sodium lauryl sulphate through an artificial oral biofilm.
The biofilm mode of growth protects plaque micro-organisms against environmental attacks, such as from antimicrobials or detergents. Dental plaque is linked to enamel through the adhesion of initial colonizers. Once this link is disrupted, the entire plaque mass adhering to it detaches. Experiments in a parallel-plate flow chamber demonstrated that bacteria adhering to saliva-coated enamel could not be stimulated to detach by perfusion of the flow chamber with two traditional mouthrinses (Corsodyl and Scope), whereas perfusion with a prebrushing rinse (Plax) or its detergent components stimulated detachment from saliva-coated enamel of a wide variety of bacterial strains. Following perfusion of the flow chamber with the mouthrinses, little additional detachment of adhering bacteria by the passage of a liquid-air interface occurred. After perfusion with the prebrushing rinse, however, significant numbers of still-adhering bacteria could be stimulated to detach by passage of a liquid-air interface, indicating that Plax had weakened their adhesive bond. The ability of Plax or its detergent components to detach plaque bacteria is not always obvious from in vivo experiments, and reports on its clinical efficacy are inconsistent. Likely, antimicrobials or detergents are unable to penetrate the plaque and reach the linking film bacteria, as demonstrated here by Fourier transform infrared spectroscopy.
Landa
A S
AS
Laboratory for Materia Technica, University of Groningen, The Netherlands.
van der Mei
H C
HC
Busscher
H J
HJ
D000818:Animals; D001422:Bacterial Adhesion / Q000187:drug effects*; D018441:Biofilms / Q000187:drug effects*; D002417:Cattle; D003743:Dental Enamel / Q000187:drug effects / Q000382:microbiology*; D003773:Dental Plaque / Q000382:microbiology / Q000628:therapy; D004353:Drug Evaluation, Preclinical; D005260:Female; D006094:Gram-Positive Bacteria / Q000187:drug effects; D006801:Humans; D066298:In Vitro Techniques; D008297:Male; D009067:Mouthwashes / Q000627:therapeutic use*; D012463:Saliva / Q000382:microbiology; D012967:Sodium Dodecyl Sulfate / Q000627:therapeutic use*; D017550:Spectroscopy, Fourier Transform Infrared; D013501:Surface-Active Agents / Q000627:therapeutic use*
D009067:Mouthwashes; D013501:Surface-Active Agents; D012967:Sodium Dodecyl Sulfate