Standardized molecular typing of Candida albicans strains.
A method is presented for the standardization of Candida albicans DNA fingerprinting, which is based on Southern hybridization of EcoRI-digested chromosomal DNA with the moderately repetitive DNA element CARE-2 and the subsequent rehybridization of the blots with a molecular size marker also included in each DNA sample. This method resulted in extremely precise alignment of all strain-specific CARE-2 hybridization patterns, even when analysed on different gels, and will enhance the accuracy of genetic relationship determinations in epidemiological studies including large numbers of strains.
Lischewski
A
A
Zentrum für Infektionsforschung, Universität Würzburg, Germany.
Harmsen
D
D
Hacker
J
J
Morschhäuser
J
J
D002176:Candida albicans / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification; D016172:DNA Fingerprinting / Q000592:standards; D004271:DNA, Fungal / Q000032:analysis; D006801:Humans; D016533:Mycological Typing Techniques / Q000592:standards*; D009693:Nucleic Acid Hybridization; D012091:Repetitive Sequences, Nucleic Acid
D004271:DNA, Fungal