Superantigen-activated T cells redirected by a bispecific antibody inhibit vesicular stomatitis virus replication in vitro and in vivo.
A bispecific Ab (BsAb) that binds the TCR on T cells and the G protein of the vesicular stomatitis virus (VSV) can redirect staphylococcal enterotoxin B (SEB)-activated T cells to kill VSV-infected cells and to inhibit VSV replication in vitro. Inhibition of virus replication in our system is dependent upon the specificity of the Ab for the viral protein. IFN-gamma does not play a very important role in this phenomenon, which is mainly mediated by the release of Pfp from CD8+ T cells. We have used a Stat1 knockout mouse model in which VSV infection is lethal. Infusion of staphylococcal enterotoxin-activated B T cells and bispecific Ab significantly slowed virus progression and prolonged the survival of VSV-infected Stat1 knockout mice in vivo.
Fernandez-Sesma
A
A
Department of Microbiology, Mount Sinai School of Medicine, NY 10029, USA.
Peluso
R W
RW
Bai
X
X
Schulman
J L
JL
Levy
D E
DE
Moran
T M
TM
D000818:Animals; D018033:Antibodies, Bispecific / Q000008:administration & dosage / Q000096:biosynthesis / Q000494:pharmacology*; D000998:Antiviral Agents / Q000378:metabolism / Q000502:physiology; D002478:Cells, Cultured; D006224:Cricetinae; D003601:Cytotoxicity Tests, Immunologic; D004768:Enterotoxins / Q000008:administration & dosage / Q000235:genetics / Q000276:immunology; D007274:Injections, Intraperitoneal; D007371:Interferon-gamma / Q000378:metabolism; D008213:Lymphocyte Activation; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D018353:Rhabdoviridae Infections / Q000276:immunology* / Q000628:therapy / Q000821:virology; D013045:Species Specificity; D013211:Staphylococcus aureus / Q000276:immunology; D013280:Stomatitis / Q000276:immunology / Q000628:therapy / Q000821:virology; D018089:Superantigens / Q000008:administration & dosage / Q000235:genetics / Q000276:immunology*; D013601:T-Lymphocytes / Q000276:immunology* / Q000378:metabolism / Q000821:virology; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology; D014721:Vesicular stomatitis Indiana virus / Q000145:classification / Q000276:immunology* / Q000502:physiology; D014779:Virus Replication / Q000276:immunology*
D018033:Antibodies, Bispecific; D000998:Antiviral Agents; D004768:Enterotoxins; D018089:Superantigens; C031818:enterotoxin B, staphylococcal; D007371:Interferon-gamma