title: Fission yeast Slp1: an effector of the Mad2-dependent spindle checkpoint.
abstract: Mad2 is a component of the spindle checkpoint, which delays the onset of anaphase until all chromosomes are attached to the spindle. Mad2 formed a complex with Slp1, a WD (tryptophan-aspartic acid)-repeat protein essential for the onset of anaphase. When the physical interaction between the two proteins was disrupted, the spindle checkpoint was no longer functional. Post-anaphase events such as chromosome decondensation and the next round of DNA replication were not delayed even when the spindle assembly was incomplete. This relief of dependence appears to be a result of deregulation of ubiquitin-dependent proteolysis mediated by the anaphase-promoting complex.
authors.affiliation: Department of Radiation Oncology, Albert Einstein College of Medicine, 1300 Morris Park Avenue, Bronx, NY 10461, USA.
authors.forename: S H
authors.initials: SH
authors.lastname: Kim
authors.forename: D P
authors.initials: DP
authors.lastname: Lin
authors.forename: S
authors.initials: S
authors.lastname: Matsumoto
authors.forename: A
authors.initials: A
authors.lastname: Kitazono
authors.forename: T
authors.initials: T
authors.lastname: Matsumoto
mesh_terms: D000705:Anaphase; D064173:Anaphase-Promoting Complex-Cyclosome; D002135:Calcium-Binding Proteins / Q000235:genetics / Q000378:metabolism*; D002352:Carrier Proteins / Q000235:genetics / Q000378:metabolism*; D064199:Cdc20 Proteins; D018797:Cell Cycle Proteins; D015825:Chromosomes, Fungal / Q000378:metabolism; D004261:DNA Replication; D005656:Fungal Proteins / Q000235:genetics / Q000378:metabolism*; D005800:Genes, Fungal; D016547:Kinesins / Q000235:genetics / Q000378:metabolism; D008025:Ligases / Q000378:metabolism; D064246:Mad2 Proteins; D008677:Metaphase; D008938:Mitosis; D016297:Mutagenesis, Site-Directed; D009154:Mutation; D009687:Nuclear Proteins; D011993:Recombinant Fusion Proteins / Q000378:metabolism; D012568:Schizosaccharomyces / Q000166:cytology* / Q000235:genetics / Q000378:metabolism*; D029702:Schizosaccharomyces pombe Proteins; D008941:Spindle Apparatus / Q000378:metabolism*; D014170:Transformation, Genetic; D043743:Ubiquitin-Protein Ligase Complexes; D044767:Ubiquitin-Protein Ligases
chemical_list: D002135:Calcium-Binding Proteins; D002352:Carrier Proteins; D064199:Cdc20 Proteins; D018797:Cell Cycle Proteins; C104159:Cut7 protein, S pombe; D005656:Fungal Proteins; D064246:Mad2 Proteins; D009687:Nuclear Proteins; D011993:Recombinant Fusion Proteins; D029702:Schizosaccharomyces pombe Proteins; C577696:mad2 protein, S pombe; C577504:slp1 protein, S pombe; D043743:Ubiquitin-Protein Ligase Complexes; D064173:Anaphase-Promoting Complex-Cyclosome; D044767:Ubiquitin-Protein Ligases; D016547:Kinesins; D008025:Ligases