title: Reconstitution of hyperacetylated, DNase I-sensitive chromatin characterized by high conformational flexibility of nucleosomal DNA.
abstract: Increased acetylation at specific N-terminal lysines of core histones is a hallmark of active chromatin in vivo, yet the structural consequences of acetylation leading to increased gene activity are only poorly defined. We employed a new approach to characterize the effects of histone acetylation: A Drosophila embryo-derived cell-free system for chromatin reconstitution under physiological conditions was programmed with exogenous histones to assemble hyperacetylated or matching control chromatin of high complexity. Hyperacetylated chromatin resembled unmodified chromatin at similar nucleosome density with respect to its sensitivity toward microccal nuclease, its nucleosomal repeat length, and the incorporation of the linker histone H1. In contrast, DNA in acetylated chromatin showed an increased sensitivity toward DNase I and a surprisingly high degree of conformational flexibility upon temperature shift pointing to profound alterations of DNA/histone interactions. This successful reconstitution of accessible and flexible chromatin outside of a nucleus paves the way for a thorough analysis of the causal relationship between histone acetylation and gene function.
authors.affiliation: European Molecular Biology Laboratory, Gene Expression Programme, Meyerhofstrasse 1, 69117 Heidelberg, Germany.
authors.forename: W A
authors.initials: WA
authors.lastname: Krajewski
authors.forename: P B
authors.initials: PB
authors.lastname: Becker
mesh_terms: D000107:Acetylation; D000818:Animals; D002843:Chromatin / Q000502:physiology* / Q000648:ultrastructure; D004247:DNA / Q000378:metabolism; D003850:Deoxyribonuclease I; D004331:Drosophila melanogaster; D006657:Histones / Q000378:metabolism*; D009038:Motion; D009690:Nucleic Acid Conformation; D009707:Nucleosomes / Q000502:physiology* / Q000648:ultrastructure
chemical_list: D002843:Chromatin; D006657:Histones; D009707:Nucleosomes; D004247:DNA; D003850:Deoxyribonuclease I