title: [Two modifications of MAIPA assays for the demonstration of platelet antibodies].
abstract: The monoclonal antibody immobilization of platelet antigens assay (MAIPA), published in 1987, is now used by many laboratories for detection of platelet antibodies. False-positive results were observed with sera containing anti-mouse IgG and in cases where MAIPA was employed to characterize platelet-specific antibodies of the IgM class. Modifications of MAIPA which completely eliminate these artifacts are described.
authors.affiliation: Institut für Klinische Immunologie und Transfusionsmedizin, Justus-Liebig-Universität Giessen, Deutschland.
authors.forename: V
authors.initials: V
authors.lastname: Kiefel
authors.forename: S
authors.initials: S
authors.lastname: Scheld
authors.forename: E
authors.initials: E
authors.lastname: Freitag
authors.forename: H
authors.initials: H
authors.lastname: Kroll
authors.forename: C
authors.initials: C
authors.lastname: Mueller-Eckhardt
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal; D016824:Antigens, Human Platelet / Q000276:immunology*; D001323:Autoantibodies / Q000097:blood*; D001792:Blood Platelets / Q000276:immunology*; D005189:False Positive Reactions; D006801:Humans; D007118:Immunoassay / Q000379:methods; D007074:Immunoglobulin G / Q000097:blood; D007075:Immunoglobulin M / Q000097:blood; D051379:Mice
chemical_list: D000911:Antibodies, Monoclonal; D016824:Antigens, Human Platelet; D001323:Autoantibodies; D007074:Immunoglobulin G; D007075:Immunoglobulin M
vernacular_title: Zwei Modifikationen des MAIPA-Assays zum Nachweis thrombozytärer Antikörper.