A genetically marked I element in Drosophila melanogaster can be mobilized when ORF2 is provided in trans.
I factors in Drosophila melanogaster are non-LTR retrotransposons similar to mammalian LINEs. They transpose at very high frequencies in the germ line of SF females resulting from crosses between reactive females, devoid of active I factors, and inducer males, containing active I factors. The vermilion marked IviP2 element was designed to allow easy phenotypical screening for retrotransposition events. It is deleted in ORF2 and therefore cannot produce reverse transcriptase. IviP2 can be mobilized at very low frequencies by actively transposing I factors in the germ line of SF females. This paper shows that IviP2 can be mobilized more efficiently in the germ line of strongly reactive females in the absence of active I factors, when it is trans-complemented by the product of ORF2 synthesized from the hsp70 heat-shock promoter. This represents a promising step toward the use of marked I elements to study retrotransposition and as tools for mutagenesis.
Busseau
I
I
Centre de Génétique Moléculaire, CNRS, Gif-sur-Yvette, France. busseau@cgm.cnrs-gif.fr
Malinsky
S
S
Balakireva
M
M
Chaboissier
M C
MC
Teninges
D
D
Bucheton
A
A
D000818:Animals; D030801:Animals, Genetically Modified; D001483:Base Sequence; D004331:Drosophila melanogaster / Q000235:genetics*; D005260:Female; D005786:Gene Expression Regulation; D017344:Genes, Insect / Q000235:genetics; D018869:Heat-Shock Response; D008969:Molecular Sequence Data; D011401:Promoter Regions, Genetic / Q000235:genetics; D018626:Retroelements / Q000235:genetics*; D016415:Sequence Alignment; D012689:Sequence Homology, Nucleic Acid
D018626:Retroelements