Biotin-labeled DNA probe in a PCR-based assay increases detection sensitivity for the equine hemoparasite Babesia caballi.
A DNA probe from Babesia caballi (Bc1) was selected by antibody screening of a genomic library. The Bc1 probe hybridized specifically to B. caballi genomic DNA. A polymerase-chain-reaction-based assay for B. caballi DNA was developed from primers deduced from the probe nucleotide sequence. An amplified product of 1.6 kb was detected from as little as 500 fg B. caballi template DNA. Sensitivity increased 1000-fold when the biotin-labeled Bc1 probe was hybridized to the amplicons in a Southern blot.
Sahagun-Ruiz
A
A
Department of Veterinary Pathobiology, Texas A&M University, College Station 77843, USA.
Waghela
S D
SD
Holman
P J
PJ
Chieves
L P
LP
Wagner
G G
GG
D000818:Animals; D001403:Babesia / Q000302:isolation & purification*; D001404:Babesiosis / Q000175:diagnosis*; D001710:Biotin; D002417:Cattle; D017931:DNA Primers; D015342:DNA Probes; D016054:DNA, Protozoan / Q000032:analysis; D015698:Genomic Library; D006734:Horse Diseases; D006736:Horses; D016133:Polymerase Chain Reaction / Q000379:methods; D012680:Sensitivity and Specificity
D017931:DNA Primers; D015342:DNA Probes; D016054:DNA, Protozoan; D001710:Biotin