A major 170 kDa protein associated with bovine adrenal medulla microtubules: a member of the centrosomin family?
Microtubules isolated from bovine adrenal medulla cells contain a major 170 kDa protein (p170). p170 is heat-labile and is associated with microtubules in an ATP-insensitive manner. This protein was purified to near homogeneity using FPLC. A preparation containing purified p170 caused bundling of microtubules. By microsequencing of p170, two polypeptides were identified which appeared to be identical to a recently sequenced p167 centrosomin-related protein. Polyclonal affinity-purified anti-p170 antibody was found to immunostain microtubules and to recognize the 170 kDa polypeptide in culture cells. We suggest that p170 is a new member of a centrosomin family and is a new structural protein associated with microtubules in some cell types.
Severin
F F
FF
Institute of Protein Research, Pushchino, Moscow Region, Russian Federation.
Shanina
N A
NA
Shevchenko
A
A
Solovyanova
O B
OB
Koretsky
V V
VV
Nadezhdina
E S
ES
D000313:Adrenal Medulla / Q000737:chemistry*; D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D000970:Antineoplastic Agents / Q000494:pharmacology; D015153:Blotting, Western; D002417:Cattle; D002478:Cells, Cultured; D002845:Chromatography; D029721:Drosophila Proteins; D004591:Electrophoresis, Polyacrylamide Gel; D005455:Fluorescent Antibody Technique; D006367:HeLa Cells / Q000166:cytology; D018398:Homeodomain Proteins / Q000737:chemistry*; D006801:Humans; D007150:Immunohistochemistry; D013058:Mass Spectrometry; D018715:Microscopy, Video; D008869:Microtubule-Associated Proteins / Q000032:analysis / Q000737:chemistry* / Q000302:isolation & purification; D015739:Nocodazole / Q000494:pharmacology; D017239:Paclitaxel / Q000494:pharmacology; D017421:Sequence Analysis
D000911:Antibodies, Monoclonal; D000970:Antineoplastic Agents; D029721:Drosophila Proteins; D018398:Homeodomain Proteins; D008869:Microtubule-Associated Proteins; C098130:cnn protein, Drosophila; D017239:Paclitaxel; D015739:Nocodazole