Effect of sofalcone on adherence, production of vacuolating toxin, and induction of interleukin-8 secretion by Helicobacter pylori.
We examined the effect of sofalcone, a mucosal protective agent that has been reported to inhibit growth of Helicobacter pylori, on adherence, production of vacuolating toxin (VT), and induction of interleukin-8 (IL-8) secretion by H. pylori. Mixing VT with various concentrations (1.5-100 micrograms/ml) of sofalcone resulted in a 50% decrease in the VT titer. When toxigenic H. pylori strains were incubated in the presence of sofalcone (20 and 40 micrograms/ml), although bacterial growth was not inhibited significantly there was significant inhibition of VT production. Notable inhibition of IL-8 secretion by human gastric cancer cells (MKN 45) was detected in the presence of sofalcone (20-100 micrograms/ml) after co-incubation with H. pylori strains. In flow cytometric analysis, adherence of H. pylori strains to MKN 45 cells was significantly inhibited by treatment with sofalcone (20-60 micrograms/ml), indicating at least one reason for the inhibition of IL-8 secretion. These results show that sofalcone is an effective mucosal protective agent that inhibits both production of VT and induction of IL-8 secretion.
Kamiya
S
S
Department of Microbiology, Kyorin University School of Medicine, Tokyo, Japan.
Osaki
T
T
Kumada
J
J
Yamaguchi
H
H
Taguchi
H
H
D000897:Anti-Ulcer Agents / Q000494:pharmacology*; D001422:Bacterial Adhesion / Q000187:drug effects; D001426:Bacterial Proteins / Q000096:biosynthesis*; D001427:Bacterial Toxins / Q000096:biosynthesis*; D002599:Chalcone / Q000031:analogs & derivatives* / Q000494:pharmacology; D047188:Chalcones; D016480:Helicobacter pylori / Q000187:drug effects* / Q000378:metabolism / Q000472:pathogenicity; D006801:Humans; D016209:Interleukin-8 / Q000378:metabolism*; D014407:Tumor Cells, Cultured
D000897:Anti-Ulcer Agents; D001426:Bacterial Proteins; D001427:Bacterial Toxins; D047188:Chalcones; D016209:Interleukin-8; C074689:VacA protein, Helicobacter pylori; C035032:sofalcone; D002599:Chalcone