MT1-MMP on the cell surface causes focal degradation of gelatin films.
The membrane-type matrix metalloproteinases (MT-MMPs) are a subclass of the matrix metalloproteinase (MMP) family which uniquely possess a C-terminal transmembrane domain and are initiators of an activation cascade for progelatinase A (MMP-2). Recent studies have shown that they can also efficiently directly degrade a number of matrix macromolecules. We now show that cells expressing MT1-MMP on their cell surfaces cause subjacent proteolysis of a gelatin film and that this proteolysis is inhibited by TIMP-2 but not by TIMP-1. These data indicate that expression of MT1-MMP on the cell surface may lead to both progelatinase A activation and extracellular matrix degradation.
d'Ortho
M P
MP
INSERM U296, Faculté de Médecine, Créteil, France.
Stanton
H
H
Butler
M
M
Atkinson
S J
SJ
Murphy
G
G
Hembry
R M
RM
D000818:Animals; D000918:Antibody Specificity; D016466:CHO Cells; D002462:Cell Membrane; D002478:Cells, Cultured; D006224:Cricetinae; D005347:Fibroblasts / Q000166:cytology / Q000378:metabolism; D005780:Gelatin / Q000378:metabolism*; D006801:Humans; D007074:Immunoglobulin G / Q000276:immunology; D053504:Matrix Metalloproteinases, Membrane-Associated; D008666:Metalloendopeptidases / Q000096:biosynthesis / Q000235:genetics / Q000378:metabolism*; D012756:Sheep
D007074:Immunoglobulin G; D005780:Gelatin; D053504:Matrix Metalloproteinases, Membrane-Associated; D008666:Metalloendopeptidases