Polymeric separation media for capillary electrophoresis of nucleic acids.
The choice of polymer matrix for separating nucleic acids by capillary electrophoresis has often been arbitrary. However, considerable research in the area has led to a wealth of data exploring the key parameters of the polymer matrix that affect nucleic acid separations: polymer type, polymer molecular mass, polymer concentration, temperature, and buffer components and additives. Using this information, it is possible to use rational methods of choosing a good polymer matrix for a particular application. Further research into the properties of the mechanism of separation in polymer solutions, as well as the polymer matrix and other solution components will lead to even more efficient separations.
Sunada
W M
WM
University of California, Berkeley 94720, USA.
Blanch
H W
HW
D019075:Electrophoresis, Capillary / Q000379:methods*; D007202:Indicators and Reagents; D008962:Models, Theoretical; D009696:Nucleic Acids / Q000032:analysis*; D011108:Polymers
D007202:Indicators and Reagents; D009696:Nucleic Acids; D011108:Polymers