title: Sequence analysis of a 25-kb segment in the 17 degrees-19 degrees region of the Bacillus subtilis chromosome containing ada locus.
abstract: As a part of the Bacillus subtilis genome sequencing project, we have determined a 25-kb sequence covering the 17 degrees-19 degrees region. This region contains 26 complete open reading frames (ORFs) including the alkA and adaA/B operon, which encode genes for adaptive response to DNA alkylation. A homology search for the newly identified 21 ORFs revealed that 4 of them exhibit a significant similarity to known proteins, e.g., methicillin-resistant Staphylococcus aureus (MRSA) protein homolog, proteins involved in chloramphenicol resistance, glucosamine synthase and an ABC transporter protein. The remaining 17 ORFs did not show any significant sequence similarities to known gene products in the database.
authors.affiliation: Institute of Molecular and Cellular Biosciences, University of Tokyo, Japan.
authors.forename: H
authors.initials: H
authors.lastname: Liu
authors.forename: K
authors.initials: K
authors.lastname: Haga
authors.forename: Y
authors.initials: Y
authors.lastname: Kasahara
authors.forename: N
authors.initials: N
authors.lastname: Ogasawara
authors.forename: H
authors.initials: H
authors.lastname: Takahashi
authors.forename: H
authors.initials: H
authors.lastname: Yoshikawa
mesh_terms: D001412:Bacillus subtilis / Q000737:chemistry* / Q000235:genetics*; D001426:Bacterial Proteins / Q000737:chemistry / Q000235:genetics; D001483:Base Sequence; D002876:Chromosomes, Bacterial / Q000737:chemistry* / Q000235:genetics*; D003360:Cosmids / Q000737:chemistry; D004269:DNA, Bacterial / Q000737:chemistry*; D016366:Open Reading Frames; D016133:Polymerase Chain Reaction; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid
chemical_list: D001426:Bacterial Proteins; D004269:DNA, Bacterial