title: Lack of association between interleukin-6 production by contact allergen-activated draining lymph node cells and lymphoproliferative activity.
abstract: BACKGROUND
  Previous investigations in BALB/c strain mice have revealed that, after skin sensitization, draining lymph node cells (LNC) produce high levels of interleukin 6 (IL-6) and that the secretion of this cytokine correlates closely with the proliferative activity of LNC. The main source of IL-6 within draining lymph nodes was found to be dendritic cells (DC), most of which derive from epidermal Langerhans cells.
  
  
  OBJECTIVE
  To explore further the relationship between DC-derived IL-6 production in lymph nodes, LNC proliferative activity, and the development of contact sensitization, comparisons between BALB/c and C3H/HeN strains of mice have been conducted.
  
  
  METHODS
  Contact sensitizing potential was measured in both strains of mice as a function of lymphocyte proliferative responses (assessed by the incorporation of radiolabelled thymidine) and challenge-induced increases in ear thickness. The concentration of IL-6 in skin homogenates and the production of IL-6 by allergen-activated LNC were measured by enzyme-linked immunosorbent assay (ELISA).
  
  
  RESULTS
  In both strains of mouse, topical exposure to oxazolone, a potent contact allergen, induced a vigorous proliferative response by draining LNC and the development of skin sensitization. However, under these conditions of exposure, activated LNC prepared from mice of C3H/HeN strain failed to secrete substantial amounts of IL-6, the levels of this cytokine being on average some 20- to 40-fold less than those measured in BALB/c mice. The failure of LNC from C3H/HeN mice to secrete comparable levels of IL-6 was not attributable to a reduced ability of DC to accumulate in draining lymph nodes after skin sensitization. Nor did reduced IL-6 secretion by C3H/HeN LNC reflect a systemic inability to elaborate this cytokine. Epicutaneous exposure of C3H/HeN mice to oxazolone resulted in the induction of cutaneous IL-6 at levels similar to, or greater than, those observed after identical treatment of BALB/c strain mice.
  
  
  CONCLUSIONS
  The conclusion drawn is that there does not exist a universal association between IL-6 production in draining lymph nodes and the vigor of proliferative responses by LNC. Further, cutaneous immune responses and skin sensitization may proceed apparently normally in the absence of high levels of IL-6 production by lymph node cells.
authors.affiliation: Department of Medical Microbiology, University of Edinburgh Medical School, Edinburgh EH8 9AG, UK.
authors.forename: M B
authors.initials: MB
authors.lastname: Lappin
authors.forename: R J
authors.initials: RJ
authors.lastname: Dearman
authors.forename: M
authors.initials: M
authors.lastname: Norval
authors.forename: I
authors.initials: I
authors.lastname: Kimber
mesh_terms: D000485:Allergens; D000818:Animals; D002455:Cell Division; D002478:Cells, Cultured; D017449:Dermatitis, Allergic Contact / Q000378:metabolism* / Q000473:pathology; D004797:Enzyme-Linked Immunosorbent Assay; D015850:Interleukin-6 / Q000096:biosynthesis*; D008198:Lymph Nodes / Q000187:drug effects / Q000378:metabolism* / Q000473:pathology; D051379:Mice; D008807:Mice, Inbred BALB C; D008809:Mice, Inbred C3H; D010081:Oxazolone / Q000633:toxicity*; D012867:Skin / Q000378:metabolism
chemical_list: D000485:Allergens; D015850:Interleukin-6; D010081:Oxazolone