title: Polyvinyl alcohol-glutaraldehyde as solid-phase in ELISA for schistosomiasis.
abstract: Soluble adult Schistosoma mansoni antigen preparation (SWAP) was covalently fixed onto polyvinyl alcohol-glutaraldehyde discs and an enzyme linked-immunosorbent assay (ELISA) was set up. The best conditions for the assay were established and it was found that small amount of antigen such as 1.5 micrograms was required. A comparison between this procedure and the conventional ELISA was proceeded. A reliable method of antigen immobilization was achieved and the low prices of the employed reagents are economically attractive.
authors.affiliation: Laboratório de Immunopatologia Keizo Asami, Universidade Federal de Pernambuco (UFPE), Recife, PE, Brazil.
authors.forename: A M
authors.initials: AM
authors.lastname: Araújo
authors.forename: G H
authors.initials: GH
authors.lastname: Barbosa
authors.forename: J R
authors.initials: JR
authors.lastname: Diniz
authors.forename: E
authors.initials: E
authors.lastname: Malagueño
authors.forename: W M
authors.initials: WM
authors.lastname: Azevedo
authors.forename: L B
authors.initials: LB
authors.lastname: de Carvalho Júnior
mesh_terms: D000818:Animals; D004797:Enzyme-Linked Immunosorbent Assay; D005976:Glutaral; D006801:Humans; D011142:Polyvinyl Alcohol; D011817:Rabbits; D012552:Schistosomiasis / Q000175:diagnosis*
chemical_list: D011142:Polyvinyl Alcohol; D005976:Glutaral