Molecular cloning and partial characterization of a tobacco cDNA encoding a small bZIP protein.
Here we report the isolation of a tobacco cDNA which is a counterpart gene of rice low-temperature-induced lip19. Expression of the gene (termed tbz17) is also positively controlled by low temperature but fairly moderately compared with the low-temperature-responsiveness of rice lip19 and the maize counterpart gene, mlip15. The predicted gene product (termed TBZ17) is a bZIP protein of 145 amino acids and shows about 45% identity with rice LIP19 and maize mLIP15 proteins. DNA binding studies indicate that TBZ17 has quite similar DNA binding characteristics to that of mLIP15. The results suggest that low-temperature-induced gene(s) encoding a DNA binding factor with bZIP motif is omni-present in higher plants.
Kusano
T
T
Biotechnology Institute, Akita Prefectural College of Agriculture, Ohgata, Japan.
Sugawara
K
K
Harada
M
M
Berberich
T
T
D000595:Amino Acid Sequence; D001483:Base Sequence; D050976:Basic-Leucine Zipper Transcription Factors; D003001:Cloning, Molecular; D018076:DNA, Complementary; D018744:DNA, Plant / Q000378:metabolism; D004268:DNA-Binding Proteins / Q000235:genetics*; D006657:Histones / Q000235:genetics; D016350:Leucine Zippers / Q000235:genetics*; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D010940:Plant Proteins / Q000235:genetics*; D010947:Plants, Toxic; D017386:Sequence Homology, Amino Acid; D014026:Nicotiana / Q000235:genetics*
D050976:Basic-Leucine Zipper Transcription Factors; D018076:DNA, Complementary; D018744:DNA, Plant; D004268:DNA-Binding Proteins; D006657:Histones; C082457:Lip19 protein, Oryza sativa; D010940:Plant Proteins