title: Serological diagnosis of chlamydial abortion in sheep and goats: comparison of the complement fixation test and an enzyme-linked immunosorbent assay employing solubilised proteins as antigen.
abstract: A new ELISA for antibodies against chlamydial abortion of ewes which uses detergent solubilised proteins (dsp) of Chlamydia psittaci as antigen (Anderson, I.E., Herring, A.J., Jones, G.E., Low, J.C., Greig, A., 1995. Development and evaluation of an indirect ELISA to detect antibodies to abortion strains of Chlamydia psittaci in sheep sera. Vet. Microbiol., 43, pp. 1-12] was compared with the complement fixation test (CFT) in screening 1000 ovine and caprine sera obtained from selected flocks/herds ('flocks') and submitted to a veterinary diagnostic laboratory. Fifteen of the 17 'flocks' had a history of abortion while the remaining two did not and were classified as 'negative flocks'. Infection with Chlamydia was confirmed during the study period in five 'flocks' using direct immunofluorescence and the modified Ziehl Neelsen stain on pathological material. The dspELISA and CFT identified 37 and 45 positive sera on 158 samples tested from these 'flocks'. Chlamydia antibodies were not detected in one of the two negative flocks, in two other flocks where the cause of abortion was undetermined and in three flocks in which the causes of abortion were diagnosed as Listeriosis and/or Salmonellosis. One of the 'negative flocks' yielded two positive reactors by CFT and five by dspELISA, suggesting infection with a cross-reactive subtype of C. pecorum. Of the five 'flocks' in which a definitive diagnosis from pathological material was not possible, four were positive by both serological tests, suggesting that the abortions were due to Chlamydia. The fifth flock, though negative by dspELISA and marginally positive in two samples by CFT, had experienced confirmed chlamydial abortions in previous lambing seasons, but culling and tetracycline treatment have prevented further abortions in the study period. Overall, the proportions of samples positive by CFT and dspELISA were similar (9.1% and 8.8%). These studies confirmed the value of the dspELISA as a screening test for chlamydial abortion. Furthermore, the dspELISA compared to the CFT is easier to perform, does not require reagent titration at each testing and uses automated assessment of results.
authors.affiliation: Dipartimento di Patologia Animale, Facoltà di Medicina Veterinaria di Torino, Italy. topatvet@csi.it
authors.forename: A
authors.initials: A
authors.lastname: Donn
authors.forename: G E
authors.initials: GE
authors.lastname: Jones
authors.forename: A
authors.initials: A
authors.lastname: Ruiu
authors.forename: M
authors.initials: M
authors.lastname: Ladu
authors.forename: J
authors.initials: J
authors.lastname: Machell
authors.forename: A
authors.initials: A
authors.lastname: Stancanelli
mesh_terms: D000034:Abortion, Veterinary / Q000382:microbiology*; D000818:Animals; D000907:Antibodies, Bacterial / Q000097:blood*; D002691:Chlamydophila psittaci / Q000276:immunology / Q000302:isolation & purification; D003937:Diagnosis, Differential; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods / Q000662:veterinary; D005260:Female; D015511:Goat Diseases; D006041:Goats; D008088:Listeriosis / Q000175:diagnosis / Q000662:veterinary; D011247:Pregnancy; D009956:Psittacosis / Q000175:diagnosis / Q000662:veterinary*; D015203:Reproducibility of Results; D012481:Salmonella Infections, Animal / Q000175:diagnosis; D012698:Serologic Tests / Q000379:methods / Q000662:veterinary; D012756:Sheep; D012757:Sheep Diseases
chemical_list: D000907:Antibodies, Bacterial