title: The humoral immune response in cattle after immunization with a multivalent IBR/PI3/Pasteurella haemolytica A1 leukotoxin vaccine.
abstract: A multivalent vaccine consisting of inactivated bovine herpes virus-1 (BHV-1), also known as infectious bovine rhinotracheitis virus (IBR), para-influenza type-3 virus (PI3) and the leukotoxin of Pasteurella haemolytica A1, were combined with the addition of aluminium hydroxide as adjuvant, and administered to post-weaned calves, and the serum tested for seroconversion to each antigen. Two groups of calves (n = 150 and n = 68) were used and were randomly divided into three subgroups. The first group of 150 calves were immunized with the multivalent vaccine (three batches) to test its first-stage stability (vaccine 3 months old) and the second group were immunized with the same vaccine 1 year later, in order to test its immunogenicity. A significant increase in titres occurred after the day 0 and 28 immunizations, for each of the three antigens in the multivalent vaccine, as measured on days 0 and 42. Immunoconversion occurred after immunization with the 3-month- and 1-year-old vaccine. The immunogenic stability of antigens in the vaccine was demonstrated after a 1-year period when the vaccine was kept at 4-6 degrees C.
authors.affiliation: Bacterial Vaccine Development Unit, Onderstepoort Veterinary Institute, South Africa.
authors.forename: M W
authors.initials: MW
authors.lastname: Odendaal
authors.forename: S
authors.initials: S
authors.lastname: Morris
authors.forename: E
authors.initials: E
authors.lastname: du Preez
authors.forename: H
authors.initials: H
authors.lastname: Aitchison
mesh_terms: D000818:Animals; D000907:Antibodies, Bacterial / Q000096:biosynthesis / Q000097:blood; D000914:Antibodies, Viral / Q000096:biosynthesis / Q000097:blood; D001427:Bacterial Toxins / Q000276:immunology; D001428:Bacterial Vaccines / Q000008:administration & dosage / Q000276:immunology; D002417:Cattle / Q000276:immunology*; D005098:Exotoxins / Q000276:immunology; D007242:Herpesvirus 1, Bovine / Q000276:immunology; D007114:Immunization / Q000662:veterinary*; D016978:Mannheimia haemolytica / Q000276:immunology; D018108:Respirovirus / Q000276:immunology; D017778:Vaccines, Combined / Q000008:administration & dosage / Q000276:immunology*; D015164:Vaccines, Inactivated / Q000008:administration & dosage / Q000276:immunology; D014765:Viral Vaccines / Q000008:administration & dosage / Q000276:immunology
chemical_list: D000907:Antibodies, Bacterial; D000914:Antibodies, Viral; D001427:Bacterial Toxins; D001428:Bacterial Vaccines; D005098:Exotoxins; D017778:Vaccines, Combined; D015164:Vaccines, Inactivated; D014765:Viral Vaccines; C018579:leukotoxin