Sister chromatid exchanges of human peripheral blood lymphocytes induced by N,N-diethylaniline in vitro.
N,N-Diethylaniline is a reagent used in organic synthesis and is an important intermediate in the manufacturing of dyes. To evaluate its genotoxicity, we examined whether it can induce sister chromatid exchanges (SCEs) in human lymphocytes. We found that N,N-diethylaniline significantly increased the frequency of SCEs both in the absence and presence of S-9 mix. The SCEs from cultures treated by N,N-diethylaniline in the presence of S-9 mix displayed a marked increase which was about 5-fold greater than the control. ANOVA analyses indicated that there is a dose-response relationship between doses of N,N-diethylaniline and the frequency of SCEs, especially in the presence of S-9 mix. The results suggested that N,N-diethylaniline has genotoxicity.
Li
Q
Q
Department of Hygiene and Public Health, Nippon Medical School, Tokyo, Japan. li_qing/pbl-hlth@nms.ac.jp
Minami
M
M
D000328:Adult; D000814:Aniline Compounds / Q000378:metabolism / Q000633:toxicity*; D004305:Dose-Response Relationship, Drug; D005260:Female; D006801:Humans; D008214:Lymphocytes / Q000187:drug effects*; D008297:Male; D009152:Mutagenicity Tests / Q000379:methods*; D012854:Sister Chromatid Exchange / Q000187:drug effects*; D013997:Time Factors
D000814:Aniline Compounds; C110696:N,N-diethylaniline; C085691:2,6-diethylaniline