Expression of cell-associated isoform of vascular endothelial growth factor 189 and its prognostic relevance in non-small cell lung cancer.
Vascular endothelial growth factor (VEGF) has four isoforms (VEGF121, VEGF165, VEGF189, VEGF206). We examined the expression patterns of VEGF isoforms in non-small cell lung cancer (NSCLC) by RT-PCR. The cell-associated isoform VEGF189 was more frequently expressed in NSCLC (90.5%) than in extraneoplastic lung tissue (57.6%, p=0.00004). Immunohistochemical examination confirmed VEGF protein expression in 33 of 76 NSCLC expressing VEGF189 (V189p+ group). The V189p+ group showed significantly poorer prognosis than those without VEGF189 mRNA and protein (V189p- group, p=0.01722). These results suggest that expression of the cell-associated isoform VEGF189 is closely associated with progression of NSCLC.
Oshika
Y
Y
Department of Pathology, Tokai University School of Medicine, Bohseidai, Isehara-shi, Kanagawa 259-11, Japan.
Nakamura
M
M
Tokunaga
T
T
Ozeki
Y
Y
Fukushima
Y
Y
Hatanaka
H
H
Abe
Y
Y
Yamazaki
H
H
Kijima
H
H
Tamaoki
N
N
Ueyama
Y
Y
D002289:Carcinoma, Non-Small-Cell Lung / Q000378:metabolism / Q000401:mortality / Q000473:pathology* / Q000601:surgery; D017931:DNA Primers; D016228:Endothelial Growth Factors / Q000032:analysis* / Q000096:biosynthesis*; D006801:Humans; D017403:In Situ Hybridization; D008168:Lung / Q000378:metabolism / Q000473:pathology; D008175:Lung Neoplasms / Q000378:metabolism / Q000401:mortality / Q000473:pathology* / Q000601:surgery; D008222:Lymphokines / Q000032:analysis* / Q000096:biosynthesis*; D009367:Neoplasm Staging; D009389:Neovascularization, Pathologic; D016133:Polymerase Chain Reaction; D011237:Predictive Value of Tests; D011379:Prognosis; D012333:RNA, Messenger / Q000032:analysis; D015996:Survival Rate; D013997:Time Factors; D042461:Vascular Endothelial Growth Factor A; D042442:Vascular Endothelial Growth Factors
D017931:DNA Primers; D016228:Endothelial Growth Factors; D008222:Lymphokines; D012333:RNA, Messenger; C467484:VEGFA protein, human; D042461:Vascular Endothelial Growth Factor A; D042442:Vascular Endothelial Growth Factors