title: Differential expression of rat brain phospholipase C isozymes in development and aging.
abstract: Phosphoinositide-specific phospholipase C (PLC) is a key enzyme in signal transduction. In the present study we examined developmental and aging changes in three PLC isozymes (beta 1, gamma 1, and delta 1) in the rat brain. Enzyme assays and immunoblot analyses after gel filtration chromatography of brain extracts from embryonic day 19 and postnatal 4- and 48-week rats indicated that gamma-specific activity was highest in fetal brain and decreased with aging, that beta 1-specific activity was high at 4 weeks but essentially undetected in fetal brain, and that delta 1-specific activity was high at both 4 and 48 weeks with faint detection in fetal brain. Our results suggest that the gamma 1 isozyme may be particularly involved in cell division and growth during the histo-genesis of the central nervous system, while beta 1 and delta 1 isozymes may take part in processes of its maturation and maintenance.
authors.affiliation: Department of Neurology, Faculty of Medicine, Kyoto University, Japan. i53367@sakura.kudpc.kyoto-u.ac.jp
authors.forename: S
authors.initials: S
authors.lastname: Shimohama
authors.forename: Y
authors.initials: Y
authors.lastname: Sumida
authors.forename: S
authors.initials: S
authors.lastname: Fujimoto
authors.forename: Y
authors.initials: Y
authors.lastname: Matsuoka
authors.forename: T
authors.initials: T
authors.lastname: Taniguchi
authors.forename: T
authors.initials: T
authors.lastname: Takenawa
authors.forename: J
authors.initials: J
authors.lastname: Kimura
mesh_terms: D000375:Aging / Q000378:metabolism*; D000818:Animals; D001921:Brain / Q000196:embryology / Q000201:enzymology* / Q000254:growth & development; D002540:Cerebral Cortex / Q000196:embryology / Q000201:enzymology / Q000254:growth & development; D005260:Female; D005333:Fetus / Q000201:enzymology; D005904:Glial Fibrillary Acidic Protein / Q000378:metabolism; D007527:Isoenzymes / Q000378:metabolism*; D043265:Phosphatidylinositol Diacylglycerol-Lyase; D054799:Phospholipase C beta; D054803:Phospholipase C delta; D051966:Phospholipase C gamma; D011247:Pregnancy; D051381:Rats; D017208:Rats, Wistar; D010738:Type C Phospholipases / Q000378:metabolism*
chemical_list: D005904:Glial Fibrillary Acidic Protein; D007527:Isoenzymes; D010738:Type C Phospholipases; D054799:Phospholipase C beta; D054803:Phospholipase C delta; C518035:Plcb1 protein, rat; C518026:Plcd1 protein, rat; D051966:Phospholipase C gamma; D043265:Phosphatidylinositol Diacylglycerol-Lyase