In vitro attachment of osteoblast-like cells to osteoceramic materials.
OBJECTIVE
The objective of this work was to examine osteoblast-like cell attachment and morphology in vitro to osteoceramic materials with three different surface morphologies.


METHODS
Osteoceramic composite disks were fabricated from tricalcium phosphate and magnesium-aluminate spinel (MgAl2O4) in a 50 vol% ratio. The disks were prepared with three different surface morphologies, including as-fired (irregular), etched (rough), or polished through 1 mm diamond paste (smooth). Osteoblast-like cell cultures were plated onto the prepared disks for 2 h, and the number of attached cells was determined. ANOVA and Student Newman-Kuels tests were used to test for significant differences in cell attachment (p < 0.05). SEM was used to visually evaluate the nature of the cellular adaptation on the osteoceramic surfaces.


RESULTS
Some additional surface roughening resulted from the interaction between the osteoceramic disks and the biological culture media during the attachment assay. A statistically larger number of cells was found to be attached to the etched osteoceramic surfaces compared to the as-fired and polished osteoceramic surfaces or the tissue culture plastic control. Cellular adaptation was extensive on all three osteoceramic surfaces at 2 h.


CONCLUSIONS
These results are consistent with previous in vivo work and continue to support the hypothesis that osteoceramic materials have potential for implants and bone substitute materials.
Keller
J C
JC
University of Iowa, Iowa City, USA. john-keller@iowa.edu
Collins
J G
JG
Niederauer
G G
GG
McGee
T D
TD
D017607:Aluminum Compounds; D000537:Aluminum Oxide / Q000737:chemistry; D000704:Analysis of Variance; D001672:Biocompatible Materials; D018786:Bone Substitutes / Q000737:chemistry; D002130:Calcium Phosphates / Q000737:chemistry*; D002448:Cell Adhesion / Q000502:physiology*; D002516:Ceramics / Q000737:chemistry*; D006801:Humans; D006882:Hydroxyapatites / Q000737:chemistry*; D017616:Magnesium Compounds; D008277:Magnesium Oxide / Q000737:chemistry; D008422:Materials Testing; D008855:Microscopy, Electron, Scanning; D010006:Osteoblasts / Q000502:physiology*; D013499:Surface Properties
D017607:Aluminum Compounds; D001672:Biocompatible Materials; D018786:Bone Substitutes; D002130:Calcium Phosphates; D006882:Hydroxyapatites; D017616:Magnesium Compounds; C485828:alpha-tricalcium phosphate; C485830:tetracalcium phosphate; C110424:aluminum magnesium oxide; C094280:hydroxyapatite-tricalciumphosphate composite; D008277:Magnesium Oxide; C485838:calcium phosphate, monobasic, anhydrous; C020243:calcium phosphate; C485829:calcium phosphate, dibasic, anhydrous; D000537:Aluminum Oxide