[Lymphadenopathy caused by Toxoplasma in an intramammary lymph node: role of molecular biology in the diagnosis].
We describe a case of Toxoplasma gondii lymphoadenopathy in an intramammary lymph node in a 22 year old woman. Clinical evidence and ultrasound were suggestive for fibroadenoma, while cytology on fine needle aspiration suspected a lymphomatous lesion. The pathological examination demonstrated a lymph node with granulomatous foci with epithelioid histiocytes, consistent with Toxoplasma gondii lymphadenopathy. The pathological finding was confirmed by serological tests and by PCR (Polymerase Chain Reaction) using specific primers designed on ribosomal 18s DNA sequence of Toxoplasma gondii. The aim of this work is to emphasize the role of molecular biology techniques as an aid to pathological diagnosis in parasite diseases.
Voglino
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G
Servizio di Anatomia ed Istologia Patologica e Citodiagnostica, Azienda Ospedaliera Materno Infantile OIRM-Sant'Anna, Ospedale Sant'Anna, Torino.
Arisio
R
R
Novero
D
D
Marchi
C
C
Fessia
L
L
D000328:Adult; D000818:Animals; D001941:Breast Diseases / Q000175:diagnosis* / Q000469:parasitology / Q000473:pathology; D001943:Breast Neoplasms / Q000175:diagnosis*; D016054:DNA, Protozoan / Q000032:analysis; D004275:DNA, Ribosomal / Q000032:analysis; D003937:Diagnosis, Differential; D005260:Female; D018226:Fibroadenoma / Q000175:diagnosis*; D006689:Hodgkin Disease / Q000175:diagnosis*; D006801:Humans; D007896:Leishmaniasis / Q000175:diagnosis; D008199:Lymphadenitis / Q000175:diagnosis* / Q000469:parasitology / Q000473:pathology; D016133:Polymerase Chain Reaction; D016053:RNA, Protozoan / Q000235:genetics; D012337:RNA, Ribosomal, 18S / Q000235:genetics; D014122:Toxoplasma / Q000235:genetics / Q000302:isolation & purification; D014123:Toxoplasmosis / Q000175:diagnosis* / Q000469:parasitology / Q000473:pathology
D016054:DNA, Protozoan; D004275:DNA, Ribosomal; D016053:RNA, Protozoan; D012337:RNA, Ribosomal, 18S
Linfoadenopatia da Toxoplasma in linfonodo intramammario: ruolo della biologia molecolare nella diagnosi.