title: A versatile method for unidirectional cloning of oligonucleotide cassettes: application to creating concatamerized transcription factor binding sites.
authors.affiliation: National Heart and Lung Institute, Imperial College School of Medicine, London, United Kingdom.
authors.forename: J F
authors.initials: JF
authors.lastname: Christer
authors.forename: M H
authors.initials: MH
authors.lastname: Yacoub
authors.forename: N J
authors.initials: NJ
authors.lastname: Brand
mesh_terms: D001483:Base Sequence; D001665:Binding Sites / Q000235:genetics; D003001:Cloning, Molecular / Q000379:methods*; D004268:DNA-Binding Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism; D005822:Genetic Vectors; D009841:Oligonucleotides / Q000235:genetics*; D010957:Plasmids / Q000235:genetics; D017422:Sequence Analysis, DNA; D014157:Transcription Factors / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D016335:Zinc Fingers / Q000235:genetics
chemical_list: D004268:DNA-Binding Proteins; D009841:Oligonucleotides; D014157:Transcription Factors