title: Osteoblast-like properties of human periodontal ligament cells: an in vitro analysis.
abstract: Identifying the biological properties of the cells residing within the periodontal ligament (PDL) will help in understanding the role that these cells play in the various functions of the periodontal ligament, and will improve the success of clinical procedures such as orthodontic tooth movement. For this purpose, fibroblasts isolated from human periodontium were cultured and characterized both histochemically and biochemically with respect to their putative osteoblast-like properties. Histochemically, cultured PDL fibroblasts showed an intense staining for alkaline phosphatase (ALP). Biochemically, the basal ALP activity increased in culture over time. ALP levels after stimulation with 1 alpha, 25-dihydroxyvitamin D3 were significantly higher than those of control cultures. Moreover, immunofluorescence against osteocalcin (a highly reliable osteoblastic marker) was strongly positive. Von Kossa staining of the cell cultures revealed the formation of mineral-like nodules. These results indicate that human PDL fibroblasts exhibit in vitro phenotypic characteristics consistent with osteoblast-like cells, thus suggesting that such cells have the potential to differentiate into osteoblasts and/or cementoblasts.
authors.affiliation: Department of Orthodontics and Dentofacial Orthopedics, School of Dental Medicine, University of Heidelberg, Germany.
authors.forename: E K
authors.initials: EK
authors.lastname: Basdra
authors.forename: G
authors.initials: G
authors.lastname: Komposch
mesh_terms: D000469:Alkaline Phosphatase / Q000032:analysis; D001669:Biochemical Phenomena; D001671:Biochemistry; D015415:Biomarkers / Q000032:analysis; D002113:Calcification, Physiologic; D002117:Calcitriol / Q000494:pharmacology; D002454:Cell Differentiation; D002478:Cells, Cultured; D004396:Coloring Agents; D003739:Dental Cementum / Q000166:cytology / Q000502:physiology; D005347:Fibroblasts / Q000166:cytology / Q000187:drug effects / Q000201:enzymology / Q000502:physiology*; D019085:Fluorescent Antibody Technique, Direct; D006651:Histocytochemistry; D006801:Humans; D008903:Minerals / Q000032:analysis; D010006:Osteoblasts / Q000166:cytology / Q000187:drug effects / Q000201:enzymology / Q000502:physiology*; D015675:Osteocalcin / Q000032:analysis; D010513:Periodontal Ligament / Q000166:cytology* / Q000187:drug effects / Q000201:enzymology / Q000502:physiology; D010641:Phenotype; D013234:Stem Cells / Q000502:physiology; D014087:Tooth Movement Techniques; D016896:Treatment Outcome
chemical_list: D015415:Biomarkers; D004396:Coloring Agents; D008903:Minerals; D015675:Osteocalcin; D000469:Alkaline Phosphatase; D002117:Calcitriol