Colony-stimulating factor-1 and monocyte chemotactic protein-1 chemotaxis for monocytes in the rat dental follicle.
Tooth eruption requires the influx of mononuclear cells (monocytes) into the dental follicle to form osteoclasts that resorb the alveolar bone to form an eruption pathway. Candidate molecules to attract these monocytes are colony-stimulating factor-1 (CSF-1) which is produced in the dental follicle, and monocyte chemotactic protein-1 (MCP-1), which is known to be a chemoattractant for monocytes. Using reverse transcription-polymerase chain reaction techniques, it was shown that the follicle cells of the first mandibular molar of the rat transcribe MCP-1 with maximal expression in vivo at day 3 postnatally, the time of peak expression of CSF-1 as well. This is also the day of peak influx of monocytes into the follicle. To determine if these molecules that were produced by the dental follicle were chemotactic, a chemotactic assay using a mouse monocyte cell line was conducted. CSF-1 or MCP-1 alone were found to be chemotactic for the monocytes and conditioned medium from the cultured follicle cells also was chemotactic. Incubating the conditioned medium with antibodies against either CSF-1 or MCP-1 reduced the chemotaxis. The results demonstrate that both CSF-1 and MCP-1 produced by the dental follicle are chemotactic for monocytes and that these chemoattractants might be responsible for the influx of monocytes into the follicle necessary to initiate tooth eruption.
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BG
Department of Veterinary Anatomy and Cell Biology, School of Veterinary Medicine, Louisiana State University, Baton Rouge 70803-8408, USA.
Wise
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GE
D000539:Alveolar Process / Q000502:physiology; D000818:Animals; D000906:Antibodies; D001862:Bone Resorption / Q000503:physiopathology; D002460:Cell Line; D018932:Chemokine CCL2 / Q000235:genetics / Q000276:immunology / Q000502:physiology*; D002630:Chemotactic Factors / Q000502:physiology; D002634:Chemotaxis, Leukocyte / Q000502:physiology*; D017077:Culture Media, Conditioned; D003795:Dental Sac / Q000166:cytology / Q000378:metabolism / Q000502:physiology*; D005786:Gene Expression Regulation; D016173:Macrophage Colony-Stimulating Factor / Q000235:genetics / Q000276:immunology / Q000502:physiology*; D008334:Mandible; D051379:Mice; D008963:Molar; D009000:Monocytes / Q000378:metabolism / Q000502:physiology*; D010010:Osteoclasts / Q000502:physiology; D016133:Polymerase Chain Reaction; D051381:Rats; D017207:Rats, Sprague-Dawley; D014078:Tooth Eruption / Q000502:physiology; D014158:Transcription, Genetic
D000906:Antibodies; D018932:Chemokine CCL2; D002630:Chemotactic Factors; D017077:Culture Media, Conditioned; D016173:Macrophage Colony-Stimulating Factor