Rat microglia exhibit increased density on Alzheimer's plaques in vitro.
The relationship of microglia to senile plaques was investigated by culturing glial cells derived from neonatal rat brain on cryostat sections of Alzheimer's disease (AD) or control brain. Rat microglia were identified by their uptake of DiI-acetylated LDL. Plaques were colocalized using Thioflavin-S staining. Although the number of microglia attached to AD tissue sections did not differ significantly from the number on control brain tissue, the density of microglia on senile plaques was significantly greater than on nonsenile plaque areas of the same sections. These results suggest that microglia may have a higher affinity for senile plaques than for nonsenile plaque regions of AD brain tissue and are consistent with the hypothesis that microglia respond to plaques.
Joshi
S N
SN
Department of Neurosurgery, University of Cincinnati Medical Center, Ohio 45267-0515, USA.
Crutcher
K A
KA
D000368:Aged; D000369:Aged, 80 and over; D000544:Alzheimer Disease / Q000473:pathology*; D000818:Animals; D052160:Benzothiazoles; D001921:Brain / Q000473:pathology; D002232:Carbocyanines; D002452:Cell Count; D002478:Cells, Cultured; D005456:Fluorescent Dyes; D006801:Humans; D008077:Lipoproteins, LDL / Q000493:pharmacokinetics; D017628:Microglia / Q000378:metabolism / Q000473:pathology*; D008875:Middle Aged; D058225:Plaque, Amyloid / Q000473:pathology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D012016:Reference Values; D013844:Thiazoles
D052160:Benzothiazoles; D002232:Carbocyanines; D005456:Fluorescent Dyes; D008077:Lipoproteins, LDL; D013844:Thiazoles; C032629:acetyl-LDL; C064633:1,1'-dipropyl-3,3,3',3'-tetramethylindocarbocyanine; C009462:thioflavin T