Two types of chicken 2',5'-oligoadenylate synthetase mRNA derived from alleles at a single locus.
We have isolated two types of chicken 2',5'-oligoadenylate synthetase cDNAs, A and B, which encode predicted proteins of 508 amino acids (58316 Da) and 476 amino acids (54336 Da), respectively. The region of A-protein comprising 33 amino acid residues from 385Ala to 417Cys is substituted by a single amino acid 385Tyr in B-protein. The homology between chicken and mammalian 2',5'-oligoadenylate synthetases is 49.5% over the amino-terminal 337 residues. Proteins expressed from A- and B-cDNAs in E. coli cells were both active in synthesizing 2',5'-oligoadenylate. However, the activity of B-protein was 10-15% of that of A-protein. Southern blotting hybridization indicated that the chicken synthetases are encoded by a single gene. RT-PCR and PCR analyses of RNA and DNA of chicken erythrocytes together with the sequence data of the PCR products showed that A- and B-mRNAs are derived from alleles at a single locus encoding chicken 2',5'-oligoadenylate synthetase, designated as OAS * A and OAS * B. Chickens carrying OAS * A/B produce two types of synthetase with molecular masses of 58 and 54 kDa, and those carrying OAS * A/A produce only a single type of 58 kDa.
Yamamoto
A
A
Department of Biotechnology, Kyoto Institute of Technology, Japan.
Iwata
A
A
Koh
Y
Y
Kawai
S
S
Murayama
S
S
Hamada
K
K
Maekawa
S
S
Ueda
S
S
Sokawa
Y
Y
D015088:2',5'-Oligoadenylate Synthetase / Q000235:genetics* / Q000378:metabolism; D000483:Alleles; D000595:Amino Acid Sequence; D000818:Animals; D019677:Artificial Gene Fusion; D001483:Base Sequence; D015139:Blotting, Southern; D015153:Blotting, Western; D002645:Chickens; D002874:Chromosome Mapping; D018076:DNA, Complementary; D006801:Humans; D008969:Molecular Sequence Data; D012333:RNA, Messenger; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid
D018076:DNA, Complementary; D012333:RNA, Messenger; D015088:2',5'-Oligoadenylate Synthetase