Effect of sinusoidally varying magnetic fields on cell proliferation and adenosine deaminase specific activity.
The effect of sinusoidally varying magnetic fields (SVMF) on chick embryo fibroblasts (CEF) was examined by two independent methods: 1) measurement of cell proliferation at 0.06-0.7 mT (100, 60 and 50 Hz) using a colorimetric assay (MTT); 2) monitoring of specific activity of adenosine deaminase (ADA) at 0.3 and 0.7 mT, 60 Hz. Both increased cell proliferation and reduced ADA specific activity are associated with cell transformation. The MTT test showed an increase in cell proliferation of up to 64% after a 24 h exposure to SVMF at 100 Hz, 0.7 mT. Cell proliferation at constant frequency (100 Hz) depended on SVMF intensity. Cell proliferation at constant intensity (0.7 mT) increased with increasing field frequency. At 0.7 mT, 60 Hz cell proliferation increased by 31%, 28%, and 26% when measured by hemocytometry, 3H-thymidine incorporation, and the MTT assay, respectively. ADA specific activity in CEF decreased by circa 48% on exposure to SVMF at 60 Hz, 0.3 mT for 24 h; only a statistically insignificant trend was seen at 0.7 mT, 60 Hz. Our findings showed that CEF cell proliferation and ADA specific activity were modified by SVMF. Both methods, independently, qualitatively detect a magnetic field effect.
Katsir
G
G
Department of Chemistry, Ben-Gurion University of the Negev, Beer-Sheva, Israel.
Baram
S C
SC
Parola
A H
AH
D000243:Adenosine Deaminase / Q000378:metabolism / Q000528:radiation effects*; D000818:Animals; D002455:Cell Division / Q000528:radiation effects*; D002478:Cells, Cultured; D002642:Chick Embryo; D004247:DNA / Q000096:biosynthesis; D004307:Dose-Response Relationship, Radiation; D004574:Electromagnetic Fields; D005347:Fibroblasts; D007700:Kinetics; D013936:Thymidine / Q000378:metabolism; D013997:Time Factors
D004247:DNA; D000243:Adenosine Deaminase; D013936:Thymidine