Spectroscopic study of the interaction of pazelliptine with nucleic acids.
The antitumor drug pazelliptine (PZE) binds to natural and synthetic DNA sequences at 100 mM NaCl, pH 7.0, as deduced from the absorption and fluorescence data. Scatchard plots constructed from the results obtained with poly(dG-dC)-poly(dG-dC) give binding constants of base pairs in the range (2-6) x 10(5)M(-1). The modifications in the absorption and fluorescence spectra observed when PZE binds to various polynucleotides, namely poly(dA-dT)-poly(dA-dT), poly(dA)-poly(dT), poly(dG-dC)-poly(dG-dC) and calf thymus DNA, reveal a change in the protonation state of the drug upon binding, increasing the apparent pKa of its 9-N-nitrogen atom. The PZE excited state properties serve as a sensitive probe to distinguish between homo and hetero A-T sites as well as between AT and GC sites. Fluorescence studies reveal that energy transfer occurs from polynucleotide bases to the bound PZE chromophore, a result consistent with an intercalative mode of binding of the drug to DNA. The emission is enhanced when PZE is bound to A-T base pairs (approximately 30% increase of phi(F) whereas it is quenched in the vicinity of G-C base pairs (approximately 90% decrease of phi(F)). Furthermore, the fluorescence spectrum obtained with calf thymus DNA is hardly distinguishable from that obtained with poly(dG-dC)-poly(dG-dC), suggesting a binding of PZE to G-C rich regions.
Renault
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E
Laboratoire de Photophysique Moléculaire, Université Paris-Sud, Orsay, France.
Fontaine-Aupart
M P
MP
Tfibel
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F
Gardes-Albert
M
M
Bisagni
E
E
D000970:Antineoplastic Agents / Q000494:pharmacology*; D004247:DNA / Q000138:chemical synthesis / Q000187:drug effects*; D004735:Energy Transfer; D007211:Indoles / Q000494:pharmacology*; D007546:Isoquinolines / Q000494:pharmacology*; D008956:Models, Chemical; D011131:Polyribonucleotides; D013057:Spectrum Analysis
D000970:Antineoplastic Agents; D007211:Indoles; D007546:Isoquinolines; D011131:Polyribonucleotides; C024256:pazelliptine; D004247:DNA