[Irreversible changes in cytochrome P-420 differential spectrum amplitude in microsomal membranes. New properties of Cu(II)-EDTA complex].
The influence of EDTA on the CO-differential spectra of cytochrome P-420 formed in the rat liver microsomes under the effect of 1 mM CuCl2 in the presence of dithionite has been studied. It was shown that the level of revealed cytochrome P-420 was diminished two-fold following 10 mM EDTA addition if dithionite concentration initially added to the medium was about 2 mM. Cytochrome b5 had no changes. Cytochrome P-420, formed under the effect of desoxycholate, was insensitive to EDTA, but its amount decreased after addition of CuCl2. It is supposed to proceed under chelatation of heme Fe(II).
Lemeshko
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Sukhodub
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D000818:Animals; D003300:Copper / Q000494:pharmacology*; D003580:Cytochromes / Q000032:analysis*; D015786:Cytochromes b5 / Q000032:analysis; D003840:Deoxycholic Acid / Q000494:pharmacology; D004227:Dithionite; D004492:Edetic Acid / Q000494:pharmacology*; D006418:Heme / Q000378:metabolism; D007502:Iron Chelating Agents / Q000494:pharmacology*; D008297:Male; D008862:Microsomes, Liver / Q000187:drug effects / Q000201:enzymology*; D051381:Rats; D017208:Rats, Wistar; D013053:Spectrophotometry
D003580:Cytochromes; D007502:Iron Chelating Agents; D003840:Deoxycholic Acid; D004227:Dithionite; D006418:Heme; D003300:Copper; D015786:Cytochromes b5; C013464:cytochrome P420; D004492:Edetic Acid; C029892:cupric chloride
Neobratimye izmeneniia amplitudy differentsial'nogo spektra tsitokhromaP-420 v membranakh mikrosom. Novye svołstva kompleksa Cu(II)-'EDTA.