Effect of age on the growth and response of a Drosophila cell line to moulting hormone.
Insect cell lines in culture are used for a variety of studies. In this laboratory imaginal disc cell lines have been established from primary cultures from third instar larvae, and used for a number of experiments. The effect of ageing on the morphology and physiology of Drosophila cell lines has received very little attention, although problems of genotypic or phenotypic changes in cell lines with age are recognized in other areas of animal cell culture. We tested our cell line Cl8+ for any difference in growth, morphology and response to 20-hydroxyecdysone (20HE) at different ages (passage numbers). The cells were found to multiply faster, adhere less firmly to the substrate and to lose the tendency to aggregate at higher passages. The response to 20HE in terms of cell numbers and induction of beta-galactosidase was similar at all passage numbers but morphological changes in hormone-treated cells were less obvious in the higher passages. Cell lines are likely to vary in the extent of ageing effects but workers are advised to be aware of the possibilities. We suggest the effects of age on cell lines should be established, and passage numbers noted in experimental reports.
Cottam
D M
DM
School of Biomedical Sciences, University of St Andrews, Fife, UK.
Milner
M J
MJ
D000818:Animals; D002460:Cell Line / Q000166:cytology / Q000187:drug effects / Q000201:enzymology; D016922:Cellular Senescence / Q000187:drug effects / Q000502:physiology; D002875:Chromosomes; D004330:Drosophila / Q000166:cytology* / Q000235:genetics; D004440:Ecdysone / Q000494:pharmacology*; D005697:Galactosides; D007211:Indoles; D011003:Ploidies; D001616:beta-Galactosidase / Q000378:metabolism
D005697:Galactosides; D007211:Indoles; D004440:Ecdysone; D001616:beta-Galactosidase; C044888:5-bromo-4-chloro-3-indolyl beta-galactoside