title: Time-resolved fluorescence spectroscopy of a hematoporphyrin derivative used for photodynamic therapy of cancer.
abstract: Photodynamic therapy (PDT) is a treatment modality for malignancies that, uses the accumulation of a photosensitizer in tumor tissue and a subsequent laser light irradiation to selectively destroy tumor cells. Usually PDT is carried out with a hematoporphyrin derivative (HPD) as photosensitizer. Fluorescence characteristics of the HPD Photosan-3 were determined at different concentrations with time-resolved fluorescence spectroscopy. Photosan-3 showed a concentration dependent fluorescence with one fluorescence decay time below a concentration of 10 micrograms/ml, two or three decay times at 50 and 100 micrograms/ml Photosan-3 respectively. These findings confirm results of former studies on other HPD that aggregates and dimers are probably more responsible for the photodynamic effect than monomers.
authors.affiliation: Department of Otorhinolaryngology, Head and Neck Surgery, Christian-Albrechts-University, Kiel, Germany.
authors.forename: S
authors.initials: S
authors.lastname: Gottschlich
authors.forename: B M
authors.initials: BM
authors.lastname: Lippert
authors.forename: W
authors.initials: W
authors.lastname: Schade
authors.forename: J A
authors.initials: JA
authors.lastname: Werner
mesh_terms: D005453:Fluorescence; D006415:Hematoporphyrins; D007834:Lasers; D009369:Neoplasms / Q000628:therapy*; D010778:Photochemotherapy; D017319:Photosensitizing Agents / Q000627:therapeutic use*; D013050:Spectrometry, Fluorescence; D013997:Time Factors
chemical_list: D006415:Hematoporphyrins; D017319:Photosensitizing Agents; C071875:photosan III