title: Position-specific inhibition of yeast mitochondrial transcription by a poly(T) sequence.
abstract: The 3' flanking nucleotide(s) of the octanucleotide promoter sequence regulates transcriptional efficiency of some mitochondrial genes in Saccharomyces cerevisiae. To understand this regulation the in vitro transcriptional activity of various synthetic mitochondrial promoters carrying different 3' flanking sequences was examined. The results presented here demonstrate that consecutive thymidine residues, but no other polynucleotides or secondary structure, in the promoter-proximal non-transcribed DNA strand inhibited mitochondrial transcription. The location and the number of T residues in the cluster as well as the concentration of UTP in the transcription reaction are the important factors determining this transcriptional inhibition. For example, a pair of thymidine nucleotides at positions +2 and +3 is sufficient for inactivation of mitochondrial transcription, whereas more than three consecutive thymidine nucleotides beyond these positions are required for inhibition of mitochondrial transcription. However, a cluster of six to 12 thymidine residues beyond position +11, a point where mtRNA polymerase has been shown to form a stable transcription complex, did not interfere with mitochondrial transcription. Interestingly, at low UTP concentration the mtRNA polymerase generates a large quantity of aborted initiation products on a template carrying promoter-proximal poly(T) sequence probably due to the inability of the polymerase to clear this promoter. On the other hand at high UTP concentration the same mtRNA polymerase on the same mitochondrial promoter produces a higher level of productive initiation complex. These observations suggest that the mechanism of poly(T) inhibition of mitochondrial transcription is a UTP-limited transcriptional attenuation at the promoter site, which might occur under specific physiological conditions (i.e. glucose repression-derepression, switching of aerobic-anaerobic conditions).
authors.affiliation: Department of Pathology, University of Chicago, IL 60637, USA.
authors.forename: T K
authors.initials: TK
authors.lastname: Biswas
authors.forename: G S
authors.initials: GS
authors.lastname: Getz
mesh_terms: D008928:Mitochondria / Q000235:genetics* / Q000378:metabolism*; D008954:Models, Biological; D009690:Nucleic Acid Conformation / Q000187:drug effects; D019384:Nucleic Acid Synthesis Inhibitors / Q000494:pharmacology*; D009841:Oligonucleotides / Q000235:genetics; D010442:Peptide Chain Initiation, Translational / Q000235:genetics; D011071:Poly T / Q000494:pharmacology*; D011401:Promoter Regions, Genetic / Q000235:genetics*; D012441:Saccharomyces cerevisiae / Q000235:genetics* / Q000502:physiology; D013942:Thymine Nucleotides / Q000494:pharmacology; D014158:Transcription, Genetic / Q000187:drug effects
chemical_list: D019384:Nucleic Acid Synthesis Inhibitors; D009841:Oligonucleotides; D013942:Thymine Nucleotides; D011071:Poly T