Andalusol, a diterpenoid with anti-inflammatory activity from Siderits foetens Clemen.
The anti-inflammatory activity of andalusol (ent-13(16),14-labdadiene-6 alpha,8 alpha,18-triol), a diterpenoid obtained from the acetone extract of Sideritis foetens Clemen, has been investigated. This compound was able to inhibit acute inflammatory processes induced by carrageenan or 12-O-tetradecanoylphorbol acetate (TPA) after oral or topical administration. Quantitation of the neutrophil specific marker, myeloperoxidase (MPO), demonstrated that its topical anti-inflammatory effect was associated with reduction in neutrophil infiltration into inflamed tissues. Interaction of andalusol with leukocyte functions and histamine release from mast cells was analyzed in vitro. At a concentration of 100 microM andalusol decreased beta-glucuronidase release from calcium ionophore A23187-stimulated rat peritoneal leukocytes. However, it failed to affect superoxide generation on TPA-stimulated leukocytes and it was non toxic to leukocytes up to 100 microM (assayed in terms of lactate dehydrogenase release). Andalusol produced a dose-dependent inhibition on histamine release from rat peritoneal mast cells stimulated by compound 48/80 or calcium ionophore A23187. These results suggest that andalusol possesses an anti-inflammatory profile, and it is in part responsible for the anti-inflammatory activity attributed to this plant.
Navarro
A
A
Departamento de Farmacología, Facultad de Farmacia, Universidad Complutense de Madrid, Spain.
de las Heras
B
B
Villar
A M
AM
D000818:Animals; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000302:isolation & purification / Q000494:pharmacology*; D002351:Carrageenan; D002470:Cell Survival / Q000187:drug effects; D004224:Diterpenes / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D004487:Edema / Q000139:chemically induced / Q000517:prevention & control*; D005966:Glucuronidase / Q000097:blood; D007213:Indomethacin / Q000494:pharmacology; D007249:Inflammation / Q000139:chemically induced / Q000517:prevention & control*; D007770:L-Lactate Dehydrogenase / Q000097:blood; D007962:Leukocytes / Q000166:cytology / Q000187:drug effects / Q000201:enzymology; D051379:Mice; D009504:Neutrophils / Q000187:drug effects / Q000502:physiology; D009195:Peroxidase / Q000097:blood; D010946:Plants, Medicinal; D051381:Rats; D013481:Superoxides / Q000097:blood; D013755:Tetradecanoylphorbol Acetate
D000894:Anti-Inflammatory Agents, Non-Steroidal; D004224:Diterpenes; C110596:ent-13(16),14-labdadiene-6,8,18-triol; D013481:Superoxides; D002351:Carrageenan; D007770:L-Lactate Dehydrogenase; D009195:Peroxidase; D005966:Glucuronidase; D013755:Tetradecanoylphorbol Acetate; D007213:Indomethacin