M-CSF serous rate evolution during post-transplantation days in a heterotopic heart graft model mice.
BACKGROUND
On heterotopic heart graft in mice, aged 7 weeks (C3H and B57), we investigate the variations of Macrophage Colony stimulating factor serous rate. The macrophage colony stimulating factor (M-CSF) is a cytokine involved in the immune response during transplantation.


METHODS
Five groups were determined, group 1 with a heterotopic transplant without immunosuppressive treatment (N=24); group 2 with a heterotopic transplant and Corticoid treatment after the graft (N=29); group 3 with a heterotopic transplant and cyclosporine treatment after the graft (N=34); group 4 with an isogenic transplant (N=31) and group 5 undergoing a laparotomy (N=31). The mice are sacrificed at D4, D7, D10 or D14 and the M-CSF dosage are done by ELISA method.


RESULTS
The serous rate of M-CSF is stable in the group with an isogenic transplant or with only a laparotomy. But in the group with a heterotopic transplant the M-CSF values increase (x1.5). If we use an immunosuppressive treatment the raising of M-CSF is less important. When we have a rejection graft, the serous rate of M-CSF increases but not significantly (Mann-Whitney test).


CONCLUSIONS
We conclude M-CSF seems to be a reliable index of disorder during immune response, but is not a good marker of the rejection.
Cornu
E
E
Department of Cardiovascular Surgery, University Hospital Dupuytren, Limoges, France.
Lacroix
P
P
Virot
P
P
Aldigier
J C
JC
Praloran
P
P
Christides
C
C
Laskar
M
M
D000818:Animals; D015415:Biomarkers / Q000097:blood; D004195:Disease Models, Animal; D006084:Graft Rejection / Q000097:blood; D016027:Heart Transplantation / Q000276:immunology*; D016173:Macrophage Colony-Stimulating Factor / Q000097:blood*; D008297:Male; D051379:Mice; D008809:Mice, Inbred C3H; D008810:Mice, Inbred C57BL; D011184:Postoperative Period; D013045:Species Specificity; D016042:Transplantation, Heterotopic
D015415:Biomarkers; D016173:Macrophage Colony-Stimulating Factor