title: A physical map of the Leishmania major Friedlin genome.
abstract: An extensive physical map of the Leishmania major Friedlin genome has been assembled by the combination of fingerprint analysis of a shuttle vector cosmid library and probe hybridization. The integrated data obtained for 9004 fingerprinted clones and 974 probes have placed 91.2% of the 33.58-Mb genome into contigs representing each of the 36 chromosomes. This first-generation map has already provided a suitable framework for both high-throughput DNA sequencing and functional studies of the L. major parasite.
authors.affiliation: Department of Biochemistry, Imperial College of Science, Technology and Medicine, London SW7 2AZ, UK. a.ivens@ic.ac.uk
authors.forename: A C
authors.initials: AC
authors.lastname: Ivens
authors.forename: S M
authors.initials: SM
authors.lastname: Lewis
authors.forename: A
authors.initials: A
authors.lastname: Bagherzadeh
authors.forename: L
authors.initials: L
authors.lastname: Zhang
authors.forename: H M
authors.initials: HM
authors.lastname: Chan
authors.forename: D F
authors.initials: DF
authors.lastname: Smith
mesh_terms: D000818:Animals; D016172:DNA Fingerprinting; D015342:DNA Probes; D016054:DNA, Protozoan / Q000032:analysis; D016521:Electrophoresis, Gel, Pulsed-Field; D005819:Genetic Markers; D018503:Genome, Protozoan; D018320:Leishmania major / Q000235:genetics*; D009693:Nucleic Acid Hybridization; D015183:Restriction Mapping / Q000379:methods*; D017422:Sequence Analysis, DNA
chemical_list: D015342:DNA Probes; D016054:DNA, Protozoan; D005819:Genetic Markers