title: Topological isomers of human uroguanylin: interconversion between biologically active and inactive isomers.
abstract: The solution structures of the two compounds of human uroguanylin (I and II), which were generated during disulfide bond forming reaction, were found to be topological isomers by 1H-nuclear magnetic resonance spectroscopy. These isomers are interconvertible in aqueous media at rates which vary with the pH and temperature of the solution. Because compound I is active in the cGMP producing assay, but compound II is not, this interconversion may be useful for evaluating the activity of human uroguanylin both in vivo and in vitro.
authors.affiliation: Peptide Institute, Inc., Protein Research Foundation, Minoh, Osaka, Japan. chino@prf.or.jp
authors.forename: N
authors.initials: N
authors.lastname: Chino
authors.forename: S
authors.initials: S
authors.lastname: Kubo
authors.forename: T
authors.initials: T
authors.lastname: Kitani
authors.forename: T
authors.initials: T
authors.lastname: Yoshida
authors.forename: R
authors.initials: R
authors.lastname: Tanabe
authors.forename: Y
authors.initials: Y
authors.lastname: Kobayashi
authors.forename: M
authors.initials: M
authors.lastname: Nakazato
authors.forename: K
authors.initials: K
authors.lastname: Kangawa
authors.forename: T
authors.initials: T
authors.lastname: Kimura
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D005768:Gastrointestinal Hormones; D006801:Humans; D007536:Isomerism; D008958:Models, Molecular; D008969:Molecular Sequence Data; D045265:Natriuretic Peptides; D019906:Nuclear Magnetic Resonance, Biomolecular; D010455:Peptides / Q000737:chemistry*; D010456:Peptides, Cyclic / Q000737:chemistry*; D011487:Protein Conformation; D051381:Rats; D016415:Sequence Alignment; D012996:Solutions
chemical_list: D005768:Gastrointestinal Hormones; D045265:Natriuretic Peptides; D010455:Peptides; D010456:Peptides, Cyclic; D012996:Solutions; C072685:guanylin; C084362:uroguanylin