title: Immunohistochemical localization of mast cells and mast cell-nerve interactions in oral lichen planus.
abstract: OBJECTIVE
  Mast cell mediators are likely to be involved in at least some aspects of the immunopathogenesis of oral lichen planus (OLP). The aim of this project was to map mast cell populations in OLP and identify possible sites of mast cell-nerve interactions.
  
  
  METHODS
  Monoclonal antibodies specific for tryptase and neurofilaments were used to identify mast cells and nerves respectively in an immunohistochemical study of OLP (n = 25) and normal oral buccal mucosa (NOBM) (n = 13) using a double-labelling protocol. Data analysis used paired t-test, multiway analysis of variance and Wilcoxon rank tests.
  
  
  RESULTS
  Morphometric analyses showed the greatest mast cell density in the most superficial of the three depth layers examined in OLP, an increase of 130% compared with NOBM. Mast cells associated with neurofilaments ranged from 21.9% in OLP to 10.2% in NOBM. Mean epithelial thickness was significantly lower in OLP (P < 0.001) but without a strong correlation with mast cell density.
  
  
  CONCLUSIONS
  Increased mast cell and mast cell-nerve interactions in OLP suggest both a controlling role over the lesional cell populations and a secondary role to the immune response once this becomes established.
authors.affiliation: School of Dentistry, University of Queensland, Australia.
authors.forename: Z Z
authors.initials: ZZ
authors.lastname: Zhao
authors.forename: N W
authors.initials: NW
authors.lastname: Savage
authors.forename: Z
authors.initials: Z
authors.lastname: Pujic
authors.forename: L J
authors.initials: LJ
authors.lastname: Walsh
mesh_terms: D000704:Analysis of Variance; D002452:Cell Count; D006801:Humans; D007150:Immunohistochemistry; D017676:Lichen Planus, Oral / Q000276:immunology*; D008407:Mast Cells / Q000502:physiology*; D009061:Mouth Mucosa / Q000276:immunology* / Q000294:innervation; D009412:Nerve Fibers; D015213:Neuroimmunomodulation; D009474:Neurons / Q000502:physiology; D018709:Statistics, Nonparametric