Eighteen novel human genes regionally mapped on chromosome 11.
Expression sequence tags (EST) obtained by sequencing a randomly primed cDNA library and gene signatures (GS) obtained by sequencing a 3'-directed cDNA library can identify genes that are active in the source cells. Eight ESTs and ten GSs which represent novel human genes, except for one GS, and which have been assigned to human chromosome 11 were used to select cosmids from a chromosome 11-specific cosmid library. These cosmids were regionally mapped using the fluorescence in situ hybridization technique.
Hori
N
N
Institute for Molecular and Cellular Biology, Osaka University, Japan.
Endo
Y
Y
Okubo
K
K
Murakawa
K
K
Yokoyama
M
M
Matoba
R
R
Matsubara
K
K
D000818:Animals; D002460:Cell Line; D002874:Chromosome Mapping; D002880:Chromosomes, Human, Pair 11 / Q000737:chemistry* / Q000235:genetics*; D002881:Chromosomes, Human, Pair 12; D002884:Chromosomes, Human, Pair 15; D003360:Cosmids / Q000032:analysis / Q000235:genetics; D006224:Cricetinae; D003412:Cricetulus; D018076:DNA, Complementary / Q000737:chemistry / Q000235:genetics*; D015870:Gene Expression; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D016133:Polymerase Chain Reaction; D012091:Repetitive Sequences, Nucleic Acid
D018076:DNA, Complementary