Characterization of the bovine herpesvirus 1 UL8 gene and gene products.
The bovine herpesvirus 1 (BHV-1) strain Schönböken UL8 gene and the 5' flanking region were sequenced. Comparison of the UL8 ORF with the previously reported UL8 ORF of BHV-1 strain Cooper revealed significant differences that were mainly due to three frame-shifted segments. Reanalysis of the Cooper sequence after isolation of the respective segments from genomic DNA by PCR did not confirm the discrepancies; on the contrary, our results indicate a high degree of sequence conservation between the UL8 proteins of different BHV-1 isolates. A monospecific antiserum, raised against a bacterially expressed TrpE-UL8 fusion protein, identified the 80 kDa apparent molecular mass UL8 polypeptide which is localized in the nucleus of infected cells. Analysis of transcripts and time-course studies demonstrated that the UL8 protein is translated from a delayed-early expressed 3.1 kb polyadenylated mRNA which initiates within the UL9 ORF.
Schmitt
J
J
Institute of Molecular and Cellular Virology, Friedrich-Loeffler-Institutes, Federal Research Centre for Virus Diseases of Animals, Insel Riems, Germany.
Keil
G M
GM
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D015152:Blotting, Northern; D002417:Cattle; D002460:Cell Line; D004265:DNA Helicases / Q000235:genetics* / Q000378:metabolism; D019915:DNA Primase; D004279:DNA, Viral; D005814:Genes, Viral; D007242:Herpesvirus 1, Bovine / Q000235:genetics*; D008969:Molecular Sequence Data; D012367:RNA, Viral; D013347:Subcellular Fractions; D014764:Viral Proteins
D004279:DNA, Viral; D012367:RNA, Viral; D014764:Viral Proteins; D019915:DNA Primase; C062021:helicase-primase, Human herpesvirus 1; D004265:DNA Helicases