title: A new transformation-deficient mutant of Haemophilus influenzae Rd with normal DNA uptake.
abstract: Haemophilus influenzae Rd is a gram-negative natural transformer. A mutant strain, RJ248, that has normal DNA uptake and translocation but whose transformation frequency is 300 times lower than that of wild-type H. influenzae and whose phage recombination is 8 times lower was isolated. The affected gene, comM, is induced during competence development in wild-type H. influenzae but not in RJ248.
authors.affiliation: Department of Molecular Biology and Genetics, School of Medicine, Johns Hopkins University, Baltimore, Maryland 21205, USA. mlgwinn@tigr.org
authors.forename: M L
authors.initials: ML
authors.lastname: Gwinn
authors.forename: R
authors.initials: R
authors.lastname: Ramanathan
authors.forename: H O
authors.initials: HO
authors.lastname: Smith
authors.forename: J F
authors.initials: JF
authors.lastname: Tomb
mesh_terms: D004251:DNA Transposable Elements; D006193:Haemophilus influenzae / Q000235:genetics* / Q000254:growth & development; D016254:Mutagenesis, Insertional; D011401:Promoter Regions, Genetic; D014169:Transformation, Bacterial
chemical_list: D004251:DNA Transposable Elements