title: Conversion of dengue virus replicative form RNA (RF) to replicative intermediate (RI) by nonstructural proteins NS-5 and NS-3.
abstract: Dengue viruses infect more than 100 million people each year and cause serious clinical manifestations. It is important to understand the replication of these viruses so that therapeutic and/or prophylactic agents may be designed. Dengue virus type 2 nonstructural proteins NS-5 and NS-3 were produced by in vitro transcription and translation of cloned genes. Both proteins possessed RNA-dependent RNA polymerase activity as measured by their ability to convert purified replicative form (RF) RNA to replicative intermediate (RI). The recombinant proteins, however, required one or more cellular protein(s) for their activity. Examination of NS-3 protein sequence revealed heretofore unnoticed sequence similarities with other polymerases.
authors.affiliation: Infectious Diseases Department, Naval Medical Research Institute, Bethesda, Maryland 20889, USA.
authors.forename: K
authors.initials: K
authors.lastname: Raviprakash
authors.forename: M
authors.initials: M
authors.lastname: Sinha
authors.forename: C G
authors.initials: CG
authors.lastname: Hayes
authors.forename: K R
authors.initials: KR
authors.lastname: Porter
mesh_terms: D000595:Amino Acid Sequence; D002478:Cells, Cultured; D002874:Chromosome Mapping; D003001:Cloning, Molecular; D003715:Dengue / Q000235:genetics / Q000821:virology*; D003716:Dengue Virus / Q000235:genetics / Q000502:physiology*; D005814:Genes, Viral; D008969:Molecular Sequence Data; D014176:Protein Biosynthesis; D020365:RNA Helicases; D012367:RNA, Viral / Q000378:metabolism*; D012324:RNA-Dependent RNA Polymerase / Q000235:genetics / Q000378:metabolism / Q000502:physiology; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism; D016415:Sequence Alignment; D012697:Serine Endopeptidases; D014158:Transcription, Genetic; D017361:Viral Nonstructural Proteins / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D014779:Virus Replication; D043583:Nucleoside-Triphosphatase; D053487:DEAD-box RNA Helicases; D000085242:Viral Proteases
chemical_list: D020365:RNA Helicases; D012367:RNA, Viral; D012324:RNA-Dependent RNA Polymerase; D011994:Recombinant Proteins; D012697:Serine Endopeptidases; D017361:Viral Nonstructural Proteins; C060028:NS3 protein, flavivirus; D043583:Nucleoside-Triphosphatase; D053487:DEAD-box RNA Helicases; D000085242:Viral Proteases