IL-1 beta mediates leptin induction during inflammation.
Interleukins (IL) are key mediators of the host response to infection and inflammation. Leptin is secreted by adipose tissue and plays an important role in the control of food intake. Administration of lipopolysaccharide (LPS), tumor necrosis factor (TNF), or IL-1 acutely increases leptin mRNA and protein levels. To investigate the role of IL-1 beta and IL-6 in leptin expression during inflammation, we used IL-1 beta-deficient (-/-) and IL-6 -/- mice. Mice were injected intraperitoneally with LPS or subcutaneously with turpentine, as models of systemic or local inflammation, respectively. In IL-1 beta +/+ mice, both LPS and turpentine increased leptin mRNA and circulating leptin. In contrast, neither LPS nor turpentine increased leptin levels in IL-1 beta -/- mice. In IL-6 +/+ or IL-6 -/- mice, turpentine increased leptin protein to comparable levels. We conclude that IL-1 beta is essential for leptin induction by both LPS and turpentine in mice, but IL-6 is not.
Faggioni
R
R
Metabolism Section, Veterans Affairs Medical Center, University of California, San Francisco 94121, USA.
Fantuzzi
G
G
Fuller
J
J
Dinarello
C A
CA
Feingold
K R
KR
Grunfeld
C
C
D000818:Animals; D005786:Gene Expression Regulation / Q000187:drug effects; D007249:Inflammation / Q000503:physiopathology*; D007375:Interleukin-1 / Q000172:deficiency / Q000235:genetics / Q000502:physiology*; D015850:Interleukin-6 / Q000172:deficiency / Q000235:genetics / Q000502:physiology*; D020738:Leptin; D008070:Lipopolysaccharides / Q000494:pharmacology; D051379:Mice; D008810:Mice, Inbred C57BL; D008815:Mice, Inbred Strains; D018345:Mice, Knockout; D008954:Models, Biological; D014176:Protein Biosynthesis; D012333:RNA, Messenger / Q000096:biosynthesis; D014158:Transcription, Genetic / Q000187:drug effects; D014425:Turpentine / Q000494:pharmacology
D007375:Interleukin-1; D015850:Interleukin-6; D020738:Leptin; D008070:Lipopolysaccharides; D012333:RNA, Messenger; D014425:Turpentine