[Bacterial diagnosis of tuberculosis using the PCR technique].
At the beginning of 80-ties new system BACTEC for mycobacteria culture has been introduced. So time of this culture has been shortened to 1-3 weeks. At the end 80-ties Polymerase Chain Reaction (PCR) technique was started and at present it is used in many laboratories in order to get information about genetic material of mycobacteria in different biological samples. Most often insertive factor IS 6110, located in chromosome Myc. tub. complex, is used. DNA amplification occurs in three stages: 1) DNA denaturation. 2) addition of starters (primers): 3) elongation of starter with d NTP synthesis. PCR technique identifies genetic material (DNA) of mycobacterium which is presented in a given biological sample. Positive PCR result not necessarily means that active disease takes place.
Wolska-Goszka
L
L
Katedry i Kliniki Chorób Płuc i Gruźlicy.
Nowak
A
A
Jassem
E
E
Galiński
J
J
Słomiński
J M
JM
D004269:DNA, Bacterial / Q000032:analysis; D005784:Gene Amplification; D006801:Humans; D009161:Mycobacterium / Q000235:genetics; D016133:Polymerase Chain Reaction / Q000379:methods*; D014376:Tuberculosis / Q000175:diagnosis* / Q000382:microbiology
D004269:DNA, Bacterial
Diagnostyka bakteriologiczna gruźlicy technika PCR.