In vivo measurement of lung preservation solution efficacy: comparison of LPD, UW, EC and low K+-EC following short and extended ischemia.
OBJECTIVE
The impact of storage solution composition on graft performance was evaluated following perfusion with either Euro-Collins (EC), low potassium Euro-Collins (rEC), low potassium dextran (LPD) or University of Wisconsin solution (UW) after brief (2 h) and extended ischemia (16 h) in an acute double lung transplantation model in the rat.


METHODS
Following flush perfusion and ischemia the lungs were implanted in recipient rats allowing serial assessment of graft pulmonary vascular resistance (PVR) and alveolar arterial oxygen difference (AaDO2) during 120 min of reperfusion. Graft dynamic lung compliance (DLC) was determined by separate ventilation. Final evaluation included weight gain and histology.


RESULTS
After extended ischemia LPD provided superior graft function in respect to DLC (repeated measures ANOVA; LPD versus rEC P < 0.05; versus EC P < 0.03; versus UW P < 0.05) and AaDO2 (LPD versus rEC P < 0.04; versus EC P < 0.006). The PVR was significantly lower in LPD versus UW (P < 0.05). At the end of reperfusion the weight increase amounted to 229 +/- 49% in rEC, 207 +/- 22% in EC, 115 +/- 22% in UW and 87 + 17% in LPD (LPD versus rEC P < 0.01, LPD versus EC P < 0.001). The type of preservation solution used had little impact on graft function after 2 h ischemia.


CONCLUSIONS
Low potassium dextran provides superior graft function after extended ischemia. After short ischemia the type of preservation solution used in this study had little impact on global lung function.
Hausen
B
B
Division of Thoracic and Cardiovascular Surgery, Surgical Center, Hannover Medical School, Germany.
Beuke
M
M
Schroeder
F
F
Poets
C F
CF
Hewitt
C
C
DelRossi
A J
AJ
Schäfers
H J
HJ
D000241:Adenosine; D000493:Allopurinol; D000818:Animals; D001992:Bronchoalveolar Lavage Fluid / Q000737:chemistry; D003911:Dextrans; D005978:Glutathione; D006982:Hypertonic Solutions; D007328:Insulin; D007511:Ischemia; D008168:Lung / Q000098:blood supply* / Q000473:pathology / Q000502:physiology; D016040:Lung Transplantation; D008297:Male; D019188:Organ Preservation Solutions; D010743:Phospholipids / Q000032:analysis; D011188:Potassium; D011506:Proteins / Q000032:analysis; D011887:Raffinose; D051381:Rats; D011917:Rats, Inbred Lew; D013997:Time Factors
D003911:Dextrans; C037096:Euro-Collins' solution; D006982:Hypertonic Solutions; D007328:Insulin; D019188:Organ Preservation Solutions; D010743:Phospholipids; D011506:Proteins; C055206:University of Wisconsin-lactobionate solution; D000493:Allopurinol; D005978:Glutathione; D000241:Adenosine; D011887:Raffinose; D011188:Potassium