Sequence and tissue distribution of chicken cellular nucleic acid binding protein cDNA.
We sequenced cDNAs coding for chicken cellular nucleic acid binding protein (CNBP). Two slightly different variations of the open reading frame were found, each of which translates into a protein with seven zinc finger domains. The longest transcript contains an in-frame insert of 3 bp. The sequence conservation between chick CNBP cDNAs with human, rat and mouse CNBP cDNAs is extreme, especially in the coding region, where the deduced amino acid sequence identity with human, rat and mouse CNBP is 99%. CNBP-like transcripts were also found in various tissues from insect, shrimp, fish and lizard. Regions with remarkable nucleotide conservation were also found in the 3' untranslated region, indicating important functions for these regions. Quantitative reverse transcription polymerase chain reaction (RT-PCR) indicated that in the chick, CNBP in present in all tissues examined in approximately equal ratios to total RNA. RT-PCR of total RNA isolated from different phyla indicate CNBP-like proteins are widespread throughout the animal kingdom. The extraordinary level of conservation suggests an important physiological role for CNBP.
van Heumen
W R
WR
Department of Physiology and Pharmacology, University of Queensland, Brisbane, Australia. Wvanheumen@vthrc.uq.edu.au
Claxton
C
C
Pickles
J O
JO
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002645:Chickens; D003001:Cloning, Molecular; D017124:Conserved Sequence; D018076:DNA, Complementary; D004268:DNA-Binding Proteins / Q000235:genetics* / Q000378:metabolism*; D006801:Humans; D051379:Mice; D008969:Molecular Sequence Data; D012326:RNA Splicing; D012333:RNA, Messenger; D016601:RNA-Binding Proteins; D051381:Rats; D017421:Sequence Analysis; D017386:Sequence Homology, Amino Acid; D012689:Sequence Homology, Nucleic Acid; D014018:Tissue Distribution
D018076:DNA, Complementary; D004268:DNA-Binding Proteins; D012333:RNA, Messenger; D016601:RNA-Binding Proteins; C433227:CNBP protein, human; C499018:Cnbp protein, rat; C499012:Cnbp protein, mouse