Benzo[a]pyrene metabolism by the hepatopancreas and green gland of the red swamp crayfish, Procambarus clarkii, in vitro.
The aim of this study was to determine the potential of activating the pro-carcinogen benzo[a]pyrene (B[a]P), elucidating B[a]P metabolite profiles, and to determine pyridine nucleotide-independent peroxygenase activity of Procambarus clarkii hepatopancreas and green gland microsomal cytochromes P450 in vitro. We compare these data to metabolite profiles generated with the rat (Rattus norvegicus) system. The major NAD(P)H-dependent metabolite formed by both hepatopancreas and green gland microsomal fractions was 3-OH-B[a]Pi; cumene hydroperoxide-dependent metabolism of B[a]P produced primarily B[a]P-quinones. B[a]P hydroxylase activity is limited by low microsomal NAD(P)H-dependent cytochrome P450 reductase levels and activity.
Jewell
C S
CS
Department of Biochemistry, Louisiana State University, Baton Rouge, USA.
Mayeaux
M H
MH
Winston
G W
GW
D000818:Animals; D003400:Astacoidea / Q000378:metabolism*; D001564:Benzo(a)pyrene / Q000378:metabolism*; D001579:Benzopyrene Hydroxylase / Q000378:metabolism; D001580:Benzopyrenes / Q000378:metabolism; D001711:Biotransformation; D002273:Carcinogens / Q000378:metabolism*; D003577:Cytochrome P-450 Enzyme System / Q000378:metabolism; D004064:Digestive System / Q000378:metabolism*; D005260:Female; D066298:In Vitro Techniques; D008297:Male; D008861:Microsomes / Q000378:metabolism; D006899:Mixed Function Oxygenases / Q000378:metabolism; D009249:NADP / Q000494:pharmacology; D051381:Rats
D001580:Benzopyrenes; D002273:Carcinogens; D001564:Benzo(a)pyrene; D009249:NADP; C021338:3-hydroxybenzo(a)pyrene; D003577:Cytochrome P-450 Enzyme System; D006899:Mixed Function Oxygenases; C022333:peroxygenase; D001579:Benzopyrene Hydroxylase