A model analysis of aftereffects of high-intensity DC stimulation on action potential of ventricular muscle.
The mechanism for aftereffects of high-intensity dc stimulation on ventricular muscle was studied by using Beeler-Reuter's action potential model. A leak conductance (Gpore, maximal value from 40 to 80 microS for 1 cm2 of membrane), which mimics reversible dielectric breakdown of the cell membrane by the shock, was incorporated into the model. To simulate resealing process, Gpore was assumed to decrease after the shock exponentially at a time constant (tau pore) of 5-50 s. The simulation results are qualitatively consistent with our experimental observations in guinea pig papillary muscle [1]; they include prolonged depolarization, diastolic depolarization or oscillation of membrane potential leading to a single or multiple spontaneous excitation. The phase-independence and shock intensity-dependence can also be reproduced. Analysis of current components has revealed that: 1) a large inward leak current (Ileak) is responsible for the prolonged depolarization; 2) time-dependent decay of outward current (IX1) in combination with Ileak and slow inward current (I(s)) results in diastolic depolarization or oscillation of membrane potential; 3) spontaneous excitation depends on an activation of I(s). These findings support our hypothesis that strong shocks (> 15 V/cm) will produce abnormal arrhythmogenic responses in ventricular muscle through a transient rupture of sarcolemmal membrane.
Sakuma
I
I
Department of Applied Electronic Engineering, Faculty of Science and Engineering, Tokyo Denki University, Saitama, Japan. sakuma@f.dendai.ac.jp
Haraguchi
T
T
Ohuchi
K
K
Fukui
Y
Y
Kodama
I
I
Toyama
J
J
Shibata
N
N
Hosoda
S
S
D000200:Action Potentials; D000818:Animals; D003198:Computer Simulation; D003971:Diastole / Q000502:physiology; D004553:Electric Conductivity; D004558:Electric Stimulation; D008322:Mammals; D008564:Membrane Potentials; D008955:Models, Cardiovascular; D010210:Papillary Muscles / Q000502:physiology; D015222:Sodium Channels / Q000378:metabolism; D016276:Ventricular Function
D015222:Sodium Channels