[Presence of alternatively spliced messenger RNA for HLA-A, -B and -C in platelets].
Recently we demonstrated that platelets contain specific messenger RNA (mRNA) encoding for the transmembrane HLA class I molecule (44 kDa). In this study we analyzed lymphocytes as well as platelets for the presence of alternatively spliced mRNA with deleted transmembrane domain resulting in soluble HLA class I antigens (39 kDa). PCR amplification of lymphocyte RNA led to a product specific for alternatively spliced cDNA corresponding to the presence of soluble HLA in plasma, while this product could not be obtained from platelet mRNA. These results suggest that platelets in contrast to lymphocytes are unable to synthesize soluble HLA antigens via alternative splicing.
Roushdy
J
J
Institut für Klinische Immunologie und Transfusionsmedizin, Giessen, Deutschland.
Kalb
R
R
Schubert
C
C
Santoso
S
S
D017398:Alternative Splicing; D001792:Blood Platelets / Q000276:immunology*; D015234:HLA-A Antigens / Q000096:biosynthesis*; D015235:HLA-B Antigens / Q000096:biosynthesis*; D015236:HLA-C Antigens / Q000096:biosynthesis*; D006801:Humans; D008214:Lymphocytes / Q000276:immunology; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000097:blood*
D015234:HLA-A Antigens; D015235:HLA-B Antigens; D015236:HLA-C Antigens; D012333:RNA, Messenger
Untersuchung über das Vorhandensein von alternativ gespleisster Messenger-RNA für HLA-A, -B und -C in Thrombozyten.