Establishment and characterization of immortalized human cell lines from prostatic carcinoma and benign prostatic hyperplasia.
New human prostate cell lines were developed from prostatic carcinoma (BRF-41T) and BPH (BRF-55T). Primary cultures were initiated from cellular outgrowths of explanted tissues. A serum-free medium, BRFF-HPC1, was developed for growing human prostatic cancer cells. Cell strains were immortalized with pRSV-T plasmid to generate permanent cell lines that exhibited an epithelial morphology. Both cell lines expressed the epithelial cell markers, cytokeratins 8 and 18 as well as the prostatic marker, PSA, and the androgen receptor gene. They possess the H-ras, K-ras, and p53 genes. We hope that these new human prostatic cell lines will be useful as in vitro models for cancer research.
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Biological Research Faculty and Facility, Inc., 10075-20 Tyler Place, Ijamsville, Maryland 21754, USA.
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Verma
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Kaighn
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D018929:Cell Culture Techniques / Q000379:methods; D004273:DNA, Neoplasm / Q000235:genetics; D016158:Genes, p53 / Q000235:genetics; D006801:Humans; D007621:Karyotyping; D008297:Male; D011470:Prostatic Hyperplasia / Q000235:genetics / Q000473:pathology*; D011471:Prostatic Neoplasms / Q000235:genetics / Q000473:pathology*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014407:Tumor Cells, Cultured / Q000166:cytology*
D004273:DNA, Neoplasm