[Development of enzyme biosensor based on trypsin and conductometric thin-film electrodes for protein and artificial substrates determination].
Two analytical systems based on the soluble and immobilized trypsin and conductometric thin-film electrodes were developed to measure concentration of artificial substrate and protein in solution. It was shown that these systems allow one to determine concentrations of Ha-benzoyl-L-arginin-ethyl-ester in the range of 0.1-1.0 mM and concentrations of HSA: 0.1-2.0 mg/ml with soluble and 0.1-0.8 mg/ml with immobilized trypsin.
Biloivan
O A
OA
Dziadevich
S V
SV
Soldatkin
O P
OP
Starodub
M F
MF
Iel'ska
G V
GV
D001120:Arginine / Q000031:analogs & derivatives* / Q000032:analysis; D015374:Biosensing Techniques; D002021:Buffers; D003217:Conductometry / Q000379:methods; D004591:Electrophoresis, Polyacrylamide Gel; D004800:Enzymes, Immobilized / Q000737:chemistry; D006801:Humans; D012709:Serum Albumin / Q000032:analysis*; D012710:Serum Albumin, Bovine / Q000032:analysis*; D012995:Solubility; D014357:Trypsin / Q000737:chemistry*
D002021:Buffers; D004800:Enzymes, Immobilized; D012709:Serum Albumin; D012710:Serum Albumin, Bovine; D001120:Arginine; C009727:benzoylarginine ethyl ester; D014357:Trypsin
Enzimosensor na osnovi tripsinu ta konduktometrichnykh planarnykh elektrodiv dlia viznacheniia vmistu bilkiv peptidnikh substrativ u vodnikh rozchinakh