Expression of the alpha 1, alpha 2, and beta 1 T3-receptor mRNAs in the fasted rat measured using competitive PCR.
Fasting is associated with a decrease in the maximal binding capacity of the T3-receptor in different tissues and the responsiveness of some genes to exogenous T3 is not as pronounced as normal. One possible explanation for these observations is a change in the expression of one or all of the isoform mRNAs. We set out to measure the levels of the alpha 1, alpha 2, and beta 1 isoform mRNAs in liver and heart of fasted rats using a competitive PCR method. We constructed competitors based on the isoforms themselves so the amplification efficiencies of the target and the competitor are similar. Using this method we found that during fasting levels of alpha 2 mRNA in the liver of rats increased threefold (P < 0.002) over a 48-h period, whereas alpha 1 and beta 1 did not show any change. In the heart, no change in any of the TR isoform mRNAs was found.
Bakker
O
O
Department of Endocrinology, Academic Medical Centre, Amsterdam, The Netherlands.
Razaki
H
H
de Jong
J
J
Ris-Stalpers
C
C
Wiersinga
W M
WM
D000818:Animals; D002467:Cell Nucleus / Q000378:metabolism; D004591:Electrophoresis, Polyacrylamide Gel; D005215:Fasting; D005786:Gene Expression Regulation / Q000235:genetics*; D008099:Liver / Q000378:metabolism; D008297:Male; D009206:Myocardium / Q000378:metabolism; D009838:Oligodeoxyribonucleotides / Q000235:genetics / Q000378:metabolism; D016133:Polymerase Chain Reaction; D011485:Protein Binding; D012333:RNA, Messenger / Q000032:analysis / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar; D011988:Receptors, Thyroid Hormone / Q000235:genetics / Q000378:metabolism*; D013960:Thyroid Function Tests; D013972:Thyrotropin / Q000097:blood / Q000378:metabolism; D013974:Thyroxine / Q000097:blood / Q000378:metabolism; D014284:Triiodothyronine / Q000097:blood / Q000378:metabolism
D009838:Oligodeoxyribonucleotides; D012333:RNA, Messenger; D011988:Receptors, Thyroid Hormone; D014284:Triiodothyronine; D013972:Thyrotropin; D013974:Thyroxine