Effect of age on human peripheral blood stem cells.
We evaluated the correlation between the number of peripheral blood stem cells (PBSCs) and aging by monitoring those of CD34+ cells (PB-CD34+), colony-forming unit granulocyte macrophages (PB-CFU-GM) per 1 ml of peripheral blood in normal volunteers (n=50) including 20 males and 30 females (median age 67, range 20-90). The numbers of PB-CD34+, PB-CFU-GM were monitored by flow cytometry and methylcellulose-based colony assay, respectively. An inverse correlation between the number of PB-CD34+ with age (r=-0.33, p=0.020) was found. Furthermore, the number of PB-CFU-GM also showed an inverse correlation with age (r=-0.58, p=0.003). Based on these results, it is suggested that the number of PBSCs decrease with age.
Egusa
Y
Y
Second Department of Internal Medicine, Hiroshima University School of Medicine, Kasumi 1-2-3, Minami-ku, Hiroshima 734, Japan.
Fujiwara
Y
Y
Syahruddin
E
E
Isobe
T
T
Yamakido
M
M
D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000375:Aging / Q000502:physiology*; D018952:Antigens, CD34 / Q000378:metabolism; D001772:Blood Cell Count; D003114:Colony-Forming Units Assay; D005260:Female; D005434:Flow Cytometry; D016178:Granulocyte-Macrophage Colony-Stimulating Factor / Q000378:metabolism; D006410:Hematopoiesis / Q000502:physiology; D006412:Hematopoietic Stem Cells / Q000502:physiology*; D006801:Humans; D008297:Male; D008875:Middle Aged
D018952:Antigens, CD34; D016178:Granulocyte-Macrophage Colony-Stimulating Factor