title: Metabolism of ferulic acid to vanillin. A bacterial gene of the enoyl-SCoA hydratase/isomerase superfamily encodes an enzyme for the hydration and cleavage of a hydroxycinnamic acid SCoA thioester.
abstract: A gene encoding a novel enoyl-SCoA hydratase/lyase enzyme for the hydration and nonoxidative cleavage of feruloyl-SCoA to vanillin and acetyl-SCoA was isolated and characterized from a strain of Pseudomonas fluorescens. Feruloyl-SCoA is the CoASH thioester of ferulic acid (4-hydroxy-3-methoxy-trans-cinnamic acid), an abundant constituent of plant cell walls and a degradation product of lignin. The gene was isolated by a combination of mutant complementation and biochemical approaches, and its function was demonstrated by heterologous expression in Escherichia coli under the control of a T7 RNA polymerase promoter. The gene product is a member of the enoyl-SCoA hydratase/isomerase superfamily.
authors.affiliation: Department of Genetics, Institute of Food Research, Norwich Laboratory, Norwich Research Park, Colney, Norwich NR4 7UA, United Kingdom.
authors.forename: M J
authors.initials: MJ
authors.lastname: Gasson
authors.forename: Y
authors.initials: Y
authors.lastname: Kitamura
authors.forename: W R
authors.initials: WR
authors.lastname: McLauchlan
authors.forename: A
authors.initials: A
authors.lastname: Narbad
authors.forename: A J
authors.initials: AJ
authors.lastname: Parr
authors.forename: E L
authors.initials: EL
authors.lastname: Parsons
authors.forename: J
authors.initials: J
authors.lastname: Payne
authors.forename: M J
authors.initials: MJ
authors.lastname: Rhodes
authors.forename: N J
authors.initials: NJ
authors.lastname: Walton
mesh_terms: D000105:Acetyl Coenzyme A / Q000378:metabolism; D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000737:chemistry; D001483:Base Sequence; D001547:Benzaldehydes / Q000378:metabolism*; D003001:Cloning, Molecular; D003373:Coumaric Acids / Q000378:metabolism*; D004746:Enoyl-CoA Hydratase / Q000737:chemistry*; D004926:Escherichia coli / Q000235:genetics; D015870:Gene Expression / Q000235:genetics; D008969:Molecular Sequence Data; D016296:Mutagenesis / Q000235:genetics; D011551:Pseudomonas fluorescens / Q000201:enzymology*; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA
chemical_list: D001426:Bacterial Proteins; D001547:Benzaldehydes; D003373:Coumaric Acids; D000105:Acetyl Coenzyme A; C004999:ferulic acid; C100058:vanillin; D004746:Enoyl-CoA Hydratase