Laser-induced modification of DNA and Poly[A,G] at guanine moiety using acetone as photosensitizer.
The interactions of triplet acetone with polyadenylic acid (Poly[A]), polyguanylic acid (Poly[G]), polyadenylic-guanylic acid (Poly[A,G]) and single-stranded DNA (ssDNA) were investigated in neutral aqueous solution using KrF (248 nm) laser flash photolysis. The transient absorption spectra and kinetics of DNA and polynucleotides obtained under acetone sensitization demonstrated that the predominant transient species was guanine radical. These novel findings have offered time-resolved evidence for photochemical modification of DNA and Poly[A,G] at guanine moiety.
Song
Q H
QH
Laboratory of Radiation Chemistry, Shanghai Institute of Nuclear Research, Academia Sinica, People's Republic of China.
Yao
S D
SD
Lin
N Y
NY
D000096:Acetone / Q000494:pharmacology*; D004247:DNA / Q000187:drug effects* / Q000528:radiation effects*; D004277:DNA, Single-Stranded / Q000187:drug effects / Q000528:radiation effects; D006147:Guanine / Q000737:chemistry* / Q000528:radiation effects; D007834:Lasers; D010782:Photolysis; D017319:Photosensitizing Agents / Q000494:pharmacology*; D011061:Poly A / Q000737:chemistry / Q000528:radiation effects; D011068:Poly G / Q000737:chemistry / Q000528:radiation effects; D011089:Polydeoxyribonucleotides / Q000737:chemistry / Q000528:radiation effects*
D004277:DNA, Single-Stranded; D017319:Photosensitizing Agents; D011089:Polydeoxyribonucleotides; C110038:polyadenylic-polyguanylic acid; D000096:Acetone; D011061:Poly A; D011068:Poly G; D006147:Guanine; D004247:DNA