Passive immune protection of turkeys against Coryza.
Antisera made against whole cells of Bordetella avium protected turkeys against disease signs of turkey coryza, but antiserum against the dermonecrotic heat-labile toxin (DHLT) did not. Neither antiserum against whole cells nor antiserum against DHLT protected turkeys against colonization of the trachea by B. avium. At least 25 bands in whole cell lysate of B. avium separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) reacted in immunoblots with protective antiserum. These bands occurred at < 40 kDa and > 105.3 kDa. DHLT had an isoelectric point (pI) in the range pH 6.3-6.7. Following SDS-PAGE of isoelectric-focused fractions, two bands were recognized by anti-DHLT with immunoblots of pI 6.3, pI 6.5, and pI 6.7 fractions separated by SDS-PAGE.
Rimler
R B
RB
Avian and Swine Respiratory Diseases Research Unit, United States Department of Agriculture, Agricultural Research Service, Ames, Iowa 50010, USA.
Kunkle
R A
RA
D000818:Animals; D000907:Antibodies, Bacterial / Q000032:analysis; D001427:Bacterial Toxins / Q000276:immunology; D001884:Bordetella / Q000276:immunology*; D001885:Bordetella Infections / Q000276:immunology / Q000517:prevention & control / Q000662:veterinary*; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods / Q000662:veterinary; D006863:Hydrogen-Ion Concentration; D007106:Immune Sera / Q000276:immunology; D007116:Immunization, Passive / Q000379:methods / Q000662:veterinary*; D015151:Immunoblotting / Q000662:veterinary; D007525:Isoelectric Focusing / Q000379:methods / Q000662:veterinary; D008297:Male; D011201:Poultry Diseases / Q000276:immunology / Q000517:prevention & control*; D014422:Turkeys
D000907:Antibodies, Bacterial; D001427:Bacterial Toxins; D007106:Immune Sera