Identification of an atypical lipoprotein-binding protein from human aortic smooth muscle as T-cadherin.
We have previously described an atypical lipoprotein-binding protein of about 105 kDa (p105) in membranes of vascular smooth muscle cells (VSMCs) that is distinct from currently known lipoprotein receptors. In the present work we have developed a procedure for purification of p105 from human aortic media. Partial sequencing of purified protein has revealed identity of p105 with human T-cadherin. Anti-peptide antisera raised against human T-cadherin recognized a protein spot corresponding to the purified p105 on two-dimensional Western blots. The antisera also inhibited LDL binding to p105 on ligand blots. We conclude that the 105 kDa lipoprotein-binding protein present in human VSMCs is T-cadherin, an unusual glycosylphosphatidylinositol-anchored member of the cadherin family of cell-cell adhesion proteins.
Tkachuk
V A
VA
Laboratory of Molecular Endocrinology, Institute of Experimental Cardiology, Cardiology Research Center, Moscow, Russia.
Bochkov
V N
VN
Philippova
M P
MP
Stambolsky
D V
DV
Kuzmenko
E S
ES
Sidorova
M V
MV
Molokoedov
A S
AS
Spirov
V G
VG
Resink
T J
TJ
D000595:Amino Acid Sequence; D000818:Animals; D001011:Aorta / Q000737:chemistry*; D015820:Cadherins / Q000737:chemistry / Q000302:isolation & purification* / Q000378:metabolism; D006801:Humans; D008077:Lipoproteins, LDL / Q000378:metabolism*; D051379:Mice; D008807:Mice, Inbred BALB C; D008969:Molecular Sequence Data; D009131:Muscle, Smooth, Vascular / Q000737:chemistry*; D043265:Phosphatidylinositol Diacylglycerol-Lyase; D011485:Protein Binding; D011817:Rabbits; D014357:Trypsin / Q000378:metabolism; D014407:Tumor Cells, Cultured; D010738:Type C Phospholipases / Q000378:metabolism
D015820:Cadherins; C099972:H-cadherin; D008077:Lipoproteins, LDL; D010738:Type C Phospholipases; D014357:Trypsin; D043265:Phosphatidylinositol Diacylglycerol-Lyase