title: Technetium-99m direct radiolabeling of monoclonal antibody ior egf/r3.
abstract: Monoclonal antibodies (MAbs) are being widely used for imaging studies, coupled mainly with 99mTc. The antibody ior egf/r3 is a MAb against human epidermal growth factor receptor (hEGF-r), and we have developed a method for optimum labeling of this MAb with 99mTc. The reduction was performed with 2-mercaptoethanol (2-ME) at a molar ratio of 2000:1 (2-ME:MAb) and methylene diphosphonate as transchelant. The integrity of reduced MAb was checked by mean of native polyacrylamide gel electrophoresis (PAGE) and gel filtration chromatography on Superose 12 (purity >99%). Radio colloids remained lower than 2%, and the labeling efficiency was 98.5%. The number of sulfhydryl groups generated was quantified using Ellman's reagent and was found to be 6.65+/-0.69 per antibody molecule. In vitro stability studies in several challenging conditions (DTPA, human serum albumin and human serum) were performed, and no significant loss in binding percentage was seen. Radio receptor assay was used to test immunoreactivity of the reduced MAb. Both labeled and unlabeled MAbs were able to compete for binding to the hEGF-r with radioiodinated EGF. Biodistribution studies in BALB/c mice are reported.
authors.affiliation: Center of Molecular Immunology, Havana, Cuba.
authors.forename: A A
authors.initials: AA
authors.lastname: Morales
authors.forename: F Z
authors.initials: FZ
authors.lastname: Crespo
authors.forename: G N
authors.initials: GN
authors.lastname: Gandolff
authors.forename: N
authors.initials: N
authors.lastname: Iznaga Escobar
authors.forename: N P
authors.initials: NP
authors.lastname: Pérez
authors.forename: J C
authors.initials: JC
authors.lastname: Hernández
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000737:chemistry* / Q000276:immunology / Q000493:pharmacokinetics; D004355:Drug Stability; D066246:ErbB Receptors / Q000276:immunology*; D005260:Female; D006801:Humans; D018796:Immunoconjugates / Q000737:chemistry* / Q000276:immunology / Q000493:pharmacokinetics; D007553:Isotope Labeling / Q000379:methods*; D008623:Mercaptoethanol / Q000737:chemistry; D051379:Mice; D008807:Mice, Inbred BALB C; D010084:Oxidation-Reduction; D013438:Sulfhydryl Compounds / Q000737:chemistry; D013667:Technetium / Q000737:chemistry*; D014018:Tissue Distribution
chemical_list: D000911:Antibodies, Monoclonal; D018796:Immunoconjugates; D013438:Sulfhydryl Compounds; D008623:Mercaptoethanol; D013667:Technetium; D066246:ErbB Receptors