Unsuspected role for the T-cell leukemia protein SCL/tal-1 in vascular development.
The transcription factor SCL/tal-1 is essential for blood cell development. Though it is also expressed in vascular endothelium, SCL has been considered dispensable for vessel formation. Through transgenic rescue of hematopoietic defects of SCL-/- embryos and analysis of chimeras generated with SCL-/- ES cells tagged with a transgene expressed in vascular endothelial cells, we show that SCL is essential for angiogenic remodeling of the yolk sac capillary network into complex vitelline vessels. These findings establish a role for SCL in embryonic angiogenesis and argue for critical functions in both embryonic blood and vascular cells, the descendents of the presumptive hemangioblast.
Visvader
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JE
Division of Hematology-Oncology, Children's Hospital and the Dana Farber Cancer Center, Department of Pediatrics, and the Howard Hughes Medical Institute, Harvard Medical School, Boston, Massachusetts 02115, USA.
Fujiwara
Y
Y
Orkin
S H
SH
D000818:Animals; D051792:Basic Helix-Loop-Helix Proteins; D002455:Cell Division; D004268:DNA-Binding Proteins / Q000235:genetics*; D004730:Endothelium, Vascular / Q000166:cytology / Q000196:embryology*; D006410:Hematopoiesis / Q000235:genetics; D051379:Mice; D008822:Mice, Transgenic; D008955:Models, Cardiovascular; D018919:Neovascularization, Physiologic / Q000235:genetics*; D011506:Proteins / Q000235:genetics; D011518:Proto-Oncogene Proteins; D022661:RNA, Untranslated; D020794:Receptor Protein-Tyrosine Kinases / Q000378:metabolism; D042787:Receptor, TIE-2; D011993:Recombinant Fusion Proteins; D000073942:T-Cell Acute Lymphocytic Leukemia Protein 1; D014157:Transcription Factors / Q000235:genetics*; D014817:Vitelline Membrane / Q000098:blood supply; D015017:Yolk Sac / Q000098:blood supply
D051792:Basic Helix-Loop-Helix Proteins; D004268:DNA-Binding Proteins; D011506:Proteins; D011518:Proto-Oncogene Proteins; D022661:RNA, Untranslated; D020794:Receptor Protein-Tyrosine Kinases; D042787:Receptor, TIE-2; D011993:Recombinant Fusion Proteins; D000073942:T-Cell Acute Lymphocytic Leukemia Protein 1; D014157:Transcription Factors; C110853:Gt(ROSA)26Sor non-coding RNA, mouse; C489366:Tal1 protein, mouse