Role of polymerase chain reaction in the diagnosis of early leprosy.
For 39 patients suspected of early leprosy, skin biopsies of the lesions were done and bisected. One piece was used for histopathologic examination and the other for polymerase chain reaction (PCR) studies to detect Mycobacterium leprae. The diagnosis of early leprosy was made clinically in 14 patients and by histopathologic study in 26 patients. Acid-fast bacilli were seen in the histopathologic sections of only two patients, and M. leprae were detected using PCR techniques in 11 patients. In one patient the diagnosis of leprosy was made only because of the detection of M. leprae in the PCR study. Since even in endemic countries the profile of leprosy is changing, detection of leprosy lesions in their early stages has become increasingly important. Since the finding of M. leprae is crucial in the confirmatory diagnosis of early leprosy, it is suggested that PCR studies to detect M. leprae be done wherever possible in conjunction with histopathologic examination. It is also recommended that the feasibility and the cost-effectiveness of both of these methods to find M. leprae be evaluated.
Job
C K
CK
Department of Leprosy, St. Thomas Hospital and Leprosy Center, T.S. District, Tamil Nadu, India.
Jayakumar
J
J
Williams
D L
DL
Gillis
T P
TP
D000293:Adolescent; D000328:Adult; D001706:Biopsy; D002648:Child; D005260:Female; D006801:Humans; D007918:Leprosy / Q000175:diagnosis*; D015439:Leprosy, Borderline / Q000175:diagnosis; D015441:Leprosy, Tuberculoid / Q000175:diagnosis; D008297:Male; D008875:Middle Aged; D009166:Mycobacterium leprae / Q000235:genetics / Q000302:isolation & purification*; D016133:Polymerase Chain Reaction / Q000379:methods*; D012867:Skin / Q000382:microbiology / Q000473:pathology