A chemiluminescent FIA biosensor for phosphate ion monitoring using pyruvate oxidase.
We have constructed an automatic phosphate ion sensing system for the quality control of drinking water. The analyte was detected using the phosphate ion-dependent pyruvate oxidase reaction and the hydrogen peroxide produced was detected by luminol chemiluminescence catalyzed by Arthromyces ramosus peroxidase. We obtained a detection limit of 0.16 microM phosphate ion (5 ppb phosphorus) and it was possible to detect 0.32 microM phosphate ion for 48 days using pyruvate oxidase immobilized on Chitopearl BCW-2601 beads. An excellent correlation (r2 = 1.00) was obtained between the results obtained using our phosphate ion sensor and those using a modified Molybdenum Blue method.
Nakamura
H
H
Research Center for Advanced Science and Technology, University of Tokyo, Japan.
Ikebukuro
K
K
McNiven
S
S
Karube
I
I
Yamamoto
H
H
Hayashi
K
K
Suzuki
M
M
Kubo
I
I
D001331:Automation; D015374:Biosensing Techniques; D004356:Drug Storage; D017022:Flow Injection Analysis; D005618:Fresh Water / Q000737:chemistry*; D007202:Indicators and Reagents; D008163:Luminescent Measurements; D010710:Phosphates / Q000032:analysis*; D011771:Pyruvate Oxidase; D011786:Quality Control
D007202:Indicators and Reagents; D010710:Phosphates; D011771:Pyruvate Oxidase