title: Control of the amount of free molecular iodine in iodine germicides.
abstract: Horseradish peroxidase has been used to generate iodine compositions that comprised principally free molecular iodine. The concentration of free molecular iodine in these enzyme-based compositions ranged from 44 to 63% of the thiosulphate titratable iodine; this is substantially higher than the corresponding value for the povidone-iodine preparation betadine. The biocidal efficacy of these compositions was proportional to the concentration of free molecular iodine. Iodine compositions with relatively low total iodine concentrations but high levels of free molecular iodine (20-175 ppm) killed Staphylococcus aureus and spores of Bacillus subtilis more rapidly than betadine. The effects of normal saline and these enzyme-based iodine compositions on the rate of epidermal regeneration in superficial swine wounds were comparable. These results suggest that an effective germicide containing a high level of molecular iodine need not be irritating or toxic.
authors.affiliation: Symbollon Corporation, Framingham, MA 01702, USA.
authors.forename: J
authors.initials: J
authors.lastname: Hickey
authors.forename: R
authors.initials: R
authors.lastname: Panicucci
authors.forename: Y
authors.initials: Y
authors.lastname: Duan
authors.forename: K
authors.initials: K
authors.lastname: Dinehart
authors.forename: J
authors.initials: J
authors.lastname: Murphy
authors.forename: J
authors.initials: J
authors.lastname: Kessler
authors.forename: W
authors.initials: W
authors.lastname: Gottardi
mesh_terms: D000818:Animals; D000891:Anti-Infective Agents, Local / Q000032:analysis / Q000737:chemistry* / Q000494:pharmacology* / Q000592:standards; D001412:Bacillus subtilis / Q000187:drug effects; D006735:Horseradish Peroxidase; D006801:Humans; D006861:Hydrogen Peroxide / Q000737:chemistry / Q000494:pharmacology; D007455:Iodine / Q000032:analysis / Q000737:chemistry* / Q000494:pharmacology*; D007700:Kinetics; D008826:Microbial Sensitivity Tests; D011206:Povidone-Iodine / Q000494:pharmacology; D013171:Spores, Bacterial / Q000187:drug effects; D013211:Staphylococcus aureus / Q000187:drug effects; D013552:Swine; D014945:Wound Healing / Q000187:drug effects
chemical_list: D000891:Anti-Infective Agents, Local; D011206:Povidone-Iodine; D007455:Iodine; D006861:Hydrogen Peroxide; D006735:Horseradish Peroxidase