CD34+ cells and their derivatives contain mRNA for CD4 and human immunodeficiency virus (HIV) co-receptors and are susceptible to infection with M- and T-tropic HIV.
Highly purified (>98%) CD34+ cells directly after isolation (D0) or 2 weeks in culture (D14) were CD4+ and contained mRNA for the T-tropic HIV co-receptor, CXCR-4, and minor co-receptor, CCR-2B. D14 but not D0 cells were RT-PCR positive for mRNA for the major M-tropic human immunodeficiency virus (HIV) co-receptor, CCR-5, and potential co-receptor, CCR-1. D14 and D0 cells were susceptible to T- (HXB2) and M-tropic HIV (Bal), showing greater virus production with Bal than HXB2, and with higher virus production levels in D14 compared to D0 cells. Seven days post-infection of D0 cells Bal DNA was present in CD14bright and CD14- fractions, suggesting D0 infection of diverse progenitor types. HXB2 DNA was detected in CD14bright cells alone indicating D0 infection of monocyte progenitors only. It is concluded that CD34+ cells and cultured derivatives are susceptible to M- and T-tropic HIV and this correlates in part with co-receptor expression at the mRNA level.
Carr
J M
JM
National Centre for HIV Virology Research, Infectious Diseases Laboratories, Institute of Medical and Veterinary Science, Adelaide, South Australia. JCARR@immuno.imvs.sa.gov.au
Ramshaw
H S
HS
Li
P
P
Burrell
C J
CJ
D015703:Antigens, CD / Q000032:analysis; D018952:Antigens, CD34; D015704:CD4 Antigens / Q000235:genetics / Q000378:metabolism*; D004279:DNA, Viral / Q000032:analysis; D015683:Gene Products, gag / Q000235:genetics; D006678:HIV / Q000378:metabolism / Q000502:physiology*; D016655:HIV Core Protein p24 / Q000032:analysis; D006412:Hematopoietic Stem Cells / Q000166:cytology / Q000378:metabolism* / Q000821:virology; D006801:Humans; D012333:RNA, Messenger; D054389:Receptors, CCR1; D054390:Receptors, CCR2; D019713:Receptors, CCR5 / Q000235:genetics; D019718:Receptors, CXCR4 / Q000235:genetics; D019707:Receptors, Chemokine / Q000235:genetics; D018121:Receptors, Cytokine / Q000235:genetics / Q000378:metabolism*; D015728:Receptors, HIV / Q000235:genetics / Q000378:metabolism*
D015703:Antigens, CD; D018952:Antigens, CD34; C515300:CCR1 protein, human; C515334:CCR2 protein, human; D015704:CD4 Antigens; D004279:DNA, Viral; D015683:Gene Products, gag; D016655:HIV Core Protein p24; D012333:RNA, Messenger; D054389:Receptors, CCR1; D054390:Receptors, CCR2; D019713:Receptors, CCR5; D019718:Receptors, CXCR4; D019707:Receptors, Chemokine; D018121:Receptors, Cytokine; D015728:Receptors, HIV