Identification of a neutrophil gelatinase-associated lipocalin mRNA in human pancreatic cancers using a modified signal sequence trap method.
To identify the intercellular signal-transducing proteins and receptors produced by cancer cells, we attempted to clone cDNAs encoding secreted and type I membrane proteins using a signal sequence trap (SST) method with some modifications. By screening an SST library derived from pancreatic cancer cells, we identified two secretory proteins (neutrophil gelatinase-associated lipocalin (NGAL) and lung surfactant protein D) and three membrane proteins (carcinoembryonic antigen, BiP/GRP78 and Hsa4 mitochondrion cytochrome oxidase subunit II). NGAL mRNA was expressed in eight of the pancreatic cancer cell lines and eight pancreatic cancer tissues. The expression of NGAL mRNA was also observed in colorectal and hepatic tumors.
Furutani
M
M
Department of Surgery and Surgical Basic Science, Graduate School of Medicine, Kyoto University, Japan.
Arii
S
S
Mizumoto
M
M
Kato
M
M
Imamura
M
M
D000209:Acute-Phase Proteins; D002352:Carrier Proteins / Q000235:genetics*; D018076:DNA, Complementary / Q000302:isolation & purification; D000091342:Endoplasmic Reticulum Chaperone BiP; D006801:Humans; D000071068:Lipocalin-2; D054834:Lipocalins; D015513:Oncogene Proteins; D010190:Pancreatic Neoplasms / Q000378:metabolism*; D011518:Proto-Oncogene Proteins; D012333:RNA, Messenger / Q000032:analysis*; D014407:Tumor Cells, Cultured
D000209:Acute-Phase Proteins; D002352:Carrier Proteins; D018076:DNA, Complementary; D000091342:Endoplasmic Reticulum Chaperone BiP; C000717247:HSPA5 protein, human; C489506:LCN2 protein, human; D000071068:Lipocalin-2; D054834:Lipocalins; D015513:Oncogene Proteins; D011518:Proto-Oncogene Proteins; D012333:RNA, Messenger