title: [Enzyme immunoassay for the determination of serotonin in stored platelet concentrates].
abstract: A new enzyme immunoassay (EIA) was applied on measurements of serotonin (5-HT) in stored platelet concentrates (PC). PC were prepared by apheresis of two separators (Cobe, Fresenius) or from buffy coats and stored for a period of 7 days at 22 degrees C and agitating. Measurements were done in pellets and suspension medium (plasma). Detection of 5-HT was specific and sensitive within the bounds of 2 and 1,000 ng/10(9) PLT.
authors.affiliation: Abteilung für Transfusionsmedizin, Universitätsklinikum Eppendorf, Hamburg, Deutschland.
authors.forename: I
authors.initials: I
authors.lastname: Ganschow
authors.forename: H P
authors.initials: HP
authors.lastname: Benn
authors.forename: R
authors.initials: R
authors.lastname: Fischer
authors.forename: U
authors.initials: U
authors.lastname: Cassens
authors.forename: R
authors.initials: R
authors.lastname: Dittmer
authors.forename: P
authors.initials: P
authors.lastname: Kühnl
mesh_terms: D001781:Blood Component Removal; D001792:Blood Platelets / Q000737:chemistry; D001793:Blood Preservation; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D006801:Humans; D010983:Plateletpheresis; D012701:Serotonin / Q000097:blood*; D013997:Time Factors
chemical_list: D012701:Serotonin
vernacular_title: Enzymimmunoassay zur Bestimmung von Serotonin in gelagerten Thrombozytenkonzentraten.