Screening of potential cancer preventing chemicals for induction of glutathione in rat liver cells.
With BRL 3A hepatocytes, a series of selected, potentially chemopreventive chemicals was evaluated for their capacity to elevate glutathione (GSH) levels. Since sodium selenite consistently increased GSH levels by approximately 70%, it was selected as a positive control. Of 62 test chemicals, eighteen stimulated GSH levels by >30%, but eleven of these had only a modest effect or displayed considerable toxicity. At non-toxic concentrations, seven compounds had substantial activity: black tea extract (decaffeinated), trans-chalcone, N-ethyl-9-cis-retinamide, indole-3-carbinol, dehydroepiandrosterone (DHEA) curcumin and N-(4-carboxyphenyl)retinamide. These should be considered for further development as cancer preventive agents.
White
E L
EL
Southern Research Institute, P.O. Box 55305, Birmingham, Alabama 35255-5305, USA.
Ross
L J
LJ
Schmid
S M
SM
Kelloff
G J
GJ
Steele
V E
VE
Hill
D L
DL
D000818:Animals; D016588:Anticarcinogenic Agents / Q000494:pharmacology*; D002478:Cells, Cultured; D004354:Drug Screening Assays, Antitumor; D005978:Glutathione / Q000096:biosynthesis*; D008099:Liver / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D051381:Rats; D011915:Rats, Inbred BUF
D016588:Anticarcinogenic Agents; D005978:Glutathione