Serotonin-sensitive leakage channel in Drosophila central neurons.
This article reports the analysis of a novel serotonin (5-HT)-sensitive leak channel. The 5-HT responses were recorded in acutely dissociated Drosophila adult and larval central nervous system (CNS) neurons by the patch-clamp method, in an attempt to establish a model preparation suitable for the genetic study of signal transduction underlying central neurotransmission. Focal perfusion or iontophoresis of 5-HT onto some patched neurons induced either an apparent inward or outward current. This apparent outward current is able to cause a strong hyperpolarization of the neuron. This article focuses on the predominant hyperpolarizing response, which is observed in a significant fraction of larger CNS neurons and in different developmental stages. The hyperpolarizing response is in fact mediated by inhibiting an inward leak current, which has a reversal potential around 0 mV. This 5-HT-sensitive leak current appears to be mediated mainly by one type of newly identified leak channel with a similar reversal potential of 0 mV and a conductance of 24 pS. In addition, it was also demonstrated that neurotransmitter-induced responses in both larval and adult Drosophila CNS neurons can be analyzed in this acutely dissociated preparation.
Wright
N J
NJ
Cold Spring Harbor Laboratory, New York 11724, USA.
Zhong
Y
Y
D000818:Animals; D001921:Brain / Q000166:cytology / Q000502:physiology*; D002478:Cells, Cultured; D004330:Drosophila / Q000502:physiology*; D017952:Ganglia, Invertebrate / Q000166:cytology / Q000502:physiology*; D007473:Ion Channels / Q000502:physiology*; D007814:Larva / Q000502:physiology; D008564:Membrane Potentials / Q000502:physiology; D009474:Neurons / Q000502:physiology*; D018408:Patch-Clamp Techniques; D012701:Serotonin / Q000502:physiology*
D007473:Ion Channels; D012701:Serotonin