Effects of capsaicin in temporomandibular joint arthritis in rats.
Temporomandibular joint (TMJ) arthritis was induced in female Lewis rats by unilateral injection of a suspension of heat-killed Mycobacterium butyricum in paraffin oil into the TMJ. Control rats received paraffin oil by the same route. Arthritic and control rats were pretreated either with capsaicin or denervation of the mandibular branch of the trigeminal nerve. Tissues were collected for neuropeptide extraction and analysed by radioimmunoassay and reverse-phase high-performance liquid chromatography. In all groups, the levels of substance P-(SP), calcitonin gene-related peptide- (CGRP) and neuropeptide Y- (NPY) like immunoreactivity (LI) were higher in the trigeminal ganglia than in the TMJs. In control rats, capsaicin significantly lowered the levels of SP-LI in the trigeminal ganglia and TMJ, but not CGRP-LI and NPY-LI. In the arthritic rats, capsaicin pretreatment significantly lowered the SP-LI and CGRP-LI in the trigeminal ganglia and TMJ, but not the NPY-LI. In the trigeminal ganglia the unilateral denervation significantly lowered SP-LI in control rats, and in arthritic rats SP-LI and CGRP-LI. On the denervated side of the arthritic TMJ, NPY-LI, SP-LI and CGRP- LI were significantly lowered as compared to the arthritic control rats and to the contralateral side. In this rat model, pretreatment with capsaicin and surgical denervation decreased the neuropeptide content in the trigeminal ganglia and the TMJ. The results clearly demonstrate a close interaction between increased neuropeptide release from sensory and sympathetic neurones after induction of arthritis in the rat.
Carleson
J
J
Department of Physiology and Pharmacology, Karolinska Institutet, Stockholm, Sweden.
Kogner
P
P
Bileviciute
I
I
Theodorsson
E
E
Appelgren
A
A
Appelgren
B
B
Kopp
S
S
Yousef
N
N
Lundeberg
T
T
D000818:Animals; D001168:Arthritis / Q000209:etiology / Q000378:metabolism / Q000517:prevention & control* / Q000601:surgery; D015740:Calcitonin Gene-Related Peptide / Q000032:analysis; D002211:Capsaicin / Q000627:therapeutic use*; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D003714:Denervation; D004195:Disease Models, Animal; D005260:Female; D008340:Mandibular Nerve / Q000378:metabolism / Q000601:surgery; D009475:Neurons, Afferent / Q000187:drug effects / Q000378:metabolism; D009478:Neuropeptide Y / Q000032:analysis; D009498:Neurotoxins / Q000627:therapeutic use*; D009170:Nontuberculous Mycobacteria; D011863:Radioimmunoassay; D051381:Rats; D011917:Rats, Inbred Lew; D013373:Substance P / Q000032:analysis; D013564:Sympathetic Nervous System / Q000187:drug effects / Q000378:metabolism; D013704:Temporomandibular Joint / Q000187:drug effects / Q000294:innervation / Q000378:metabolism; D013705:Temporomandibular Joint Disorders / Q000209:etiology / Q000378:metabolism / Q000517:prevention & control* / Q000601:surgery; D012668:Trigeminal Ganglion / Q000187:drug effects / Q000378:metabolism
D009478:Neuropeptide Y; D009498:Neurotoxins; D013373:Substance P; D015740:Calcitonin Gene-Related Peptide; D002211:Capsaicin