Typing of Candida albicans by use of a combined PCR/hybridization assay.
A modified genotyping assay for Candida albicans based on the RAPD-PCR (random amplification of polymorphic DNA) is described. Following capillary blot of RAPD-PCR products onto a nylon membrane, hybridization with a PCR-generated gene probe resulted in heterogeneous band patterns. By this method the discriminatory power of RAPD-PCR is enhanced with satisfactory reproducibility and reduced interference.
Vogeser
M
M
Klinikums Grosshadern, Ludwig-Maximilians-Universität München, FR Germany.
Haas
A
A
Anding
G
G
Ruckdeschel
G
G
D002176:Candida albicans / Q000145:classification*; D016533:Mycological Typing Techniques; D009693:Nucleic Acid Hybridization; D019105:Random Amplified Polymorphic DNA Technique; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity