Sequence analysis of a 45-kb segment in the 19 degrees-23 degrees region of the Bacillus subtilis chromosome containing glpT and mpr loci.
The nucleotide sequence of a 45,137-bp segment covering the 19 degrees and 23 degrees region in the 360 degrees map of the Bacillus subtilis genome was determined. This region contained 45 open reading frames (ORFs) including 7 which corresponded to the products of genes with known functions that had been previously sequenced. The known genes were: glpT and glpQ for glycerol utilization pathway; purT for a part of the purine synthesis pathway; mpr for an extracellular metalloprotease; pss and psd for the parts of the phospholipid synthesis pathway; and gltP for a glutamine transporter. Deduced amino acid sequences of the 22 newly identified ORFs showed significant homologies to known gene products in the database such as a Methicillin-resistant Staphylococcus aureus (MRSA) gene which is related to drug resistance, a two-component response regulator, a series of amino acid permeases, transcriptional regulators, beta-lactamase, the phosphotransferase system (PTS) enzyme II for sugar uptake, and the eukaryotic ECA39 gene which is associated with cancer and apoptosis, etc. The remaining 16 ORFs did not show any significant sequence similarities to known gene products in the database.
Haga
K
K
Institute of Molecular and Cellular Biosciences, University of Tokyo, Japan.
Liu
H
H
Takahashi
H
H
Yoshikawa
H
H
D000818:Animals; D001412:Bacillus subtilis / Q000737:chemistry* / Q000235:genetics*; D001426:Bacterial Proteins / Q000737:chemistry / Q000235:genetics; D001483:Base Sequence; D002876:Chromosomes, Bacterial / Q000737:chemistry* / Q000235:genetics*; D003360:Cosmids / Q000737:chemistry; D004269:DNA, Bacterial / Q000737:chemistry*; D051379:Mice; D016366:Open Reading Frames; D016133:Polymerase Chain Reaction; D051381:Rats; D017421:Sequence Analysis; D017422:Sequence Analysis, DNA
D001426:Bacterial Proteins; D004269:DNA, Bacterial