title: GM-CSF production by epithelial cell line: upregulation by ultraviolet A.
abstract: It was demonstrated that UVB increases synthesis and expression of IL-1 alpha and GM-CSF by keratinocytes. Upregulation of GM-CSF by UVB is reported to be mediated by IL-1 alpha. However, regulation of IL-1 alpha and GM-CSF by UVA is not well-known. The purpose of the present study was to evaluate the effects of UVA on IL-1 alpha and GM-CSF production. Here we used a competitive RT-PCR for measuring cytokine gene expression in an epidermal cell line after UVA irradiation. IL-1 alpha and GM-CSF mRNA did not show any change at 1 h and 6 h following exposure to UVA. After UVA irradiation, however, IL-1 alpha mRNA decreased and GM-CSF mRNA increased at 24 h and the level of GM-CSF in culture supernatant increased at 24 h and 48 h. Addition of antihuman IL-1 alpha neutralizing antibody to UVA irradiated cells did not prevent the increase of GM-CSF mRNA expression. These results suggest that UVA radiation may induce GM-CSF production through an IL-1 alpha independent pathway.
authors.affiliation: Department of Dermatology, Seoul National University College of Medicine, Korea.
authors.forename: K C
authors.initials: KC
authors.lastname: Park
authors.forename: H C
authors.initials: HC
authors.lastname: Jung
authors.forename: J H
authors.initials: JH
authors.lastname: Hwang
authors.forename: S W
authors.initials: SW
authors.lastname: Youn
authors.forename: J S
authors.initials: JS
authors.lastname: Ahn
authors.forename: S B
authors.initials: SB
authors.lastname: Park
authors.forename: K H
authors.initials: KH
authors.lastname: Kim
authors.forename: J H
authors.initials: JH
authors.lastname: Chung
authors.forename: J I
authors.initials: JI
authors.lastname: Youn
mesh_terms: D000906:Antibodies; D002460:Cell Line; D002470:Cell Survival / Q000528:radiation effects; D017931:DNA Primers; D004797:Enzyme-Linked Immunosorbent Assay; D000078404:Epidermal Cells; D005786:Gene Expression Regulation / Q000528:radiation effects; D016178:Granulocyte-Macrophage Colony-Stimulating Factor / Q000096:biosynthesis / Q000235:genetics / Q000528:radiation effects*; D006801:Humans; D007375:Interleukin-1 / Q000096:biosynthesis / Q000235:genetics / Q000276:immunology / Q000528:radiation effects; D015603:Keratinocytes / Q000378:metabolism / Q000528:radiation effects*; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000235:genetics / Q000528:radiation effects; D013997:Time Factors; D014158:Transcription, Genetic; D014466:Ultraviolet Rays / Q000145:classification*; D015854:Up-Regulation / Q000528:radiation effects*
chemical_list: D000906:Antibodies; D017931:DNA Primers; D007375:Interleukin-1; D012333:RNA, Messenger; D016178:Granulocyte-Macrophage Colony-Stimulating Factor