title: Isoforms of Serratia marcescens nuclease. The role of Mg2+ in the hydrolysis mechanism.
abstract: Structural and functional differences between isoforms Sm1 and Sm2, a lack of influence of free Mg2+ on the isoform structures, formation of DNA-magnesium complex serving with great probability as a real substrate for the nuclease has been summarized on the basis of experimental data. Mg2+ forming a complex with phosphate groups of DNA are supposed to further increase the electrophilicity of the phosphorus atoms besides causing a conformational change of the substrate.
authors.affiliation: Department of Microbiology, Kazan State University, Russia. maria.filimonova@ksu.ru
authors.forename: M N
authors.initials: MN
authors.lastname: Filimonova
authors.forename: V P
authors.initials: VP
authors.lastname: Gubskaya
authors.forename: I A
authors.initials: IA
authors.lastname: Nuretdinov
authors.forename: M J
authors.initials: MJ
authors.lastname: Benedik
authors.forename: L M
authors.initials: LM
authors.lastname: Bogomolnaya
authors.forename: M A
authors.initials: MA
authors.lastname: Andreeva
authors.forename: I B
authors.initials: IB
authors.lastname: Leshchinskaya
mesh_terms: D002942:Circular Dichroism; D004247:DNA / Q000378:metabolism; D004706:Endodeoxyribonucleases / Q000378:metabolism*; D004722:Endoribonucleases / Q000378:metabolism*; D006868:Hydrolysis; D007526:Isoelectric Point; D007527:Isoenzymes / Q000378:metabolism; D007700:Kinetics; D008274:Magnesium / Q000378:metabolism*; D011487:Protein Conformation; D012706:Serratia marcescens / Q000201:enzymology*; D013329:Structure-Activity Relationship
chemical_list: D007527:Isoenzymes; D004247:DNA; D004706:Endodeoxyribonucleases; D004722:Endoribonucleases; C021835:Serratia marcescens nuclease; D008274:Magnesium