Molecular and biological characterization of rabbit mannan-binding protein.
Mannan-binding protein (MBP) is a member of the collectin family of protein. There are two types of MBP, MBP-A and MBP-C, which were found in rodent (rats and mice), rhesus monkey, and cynomolgus monkey, while chimpanzee and human have only one MBP. It was considered that the loss of one MBP gene occurred during hominoid evolution. In this article two rabbit MBP, a liver and serum MBP, were characterized biologically and genetically. Analyses by SDS-PAGE under reduced condition and their amino acid sequences of both MBPs showed that they have a same molecular weight of 32 kDa and their amino acid sequences were identical. A serum MBP has a higher ability to activate complement than does a liver MBP; however, a liver MBP inhibits hemagglutination by influenza virus as strongly as a serum MBP does. cDNA clones encoding the rabbit MBP were isolated from a rabbit cDNA liver library using whole cDNA of mouse MBP-C as a probe. The cDNA carried an insert of 744 bp coding for a protein of 247 acid residues with a signal peptide of 22 residues. The deduced amino acid sequence of the cDNA was identical to that of amino acid sequences of the 32 kDa proteins determined here. Northern blot analysis showed that mRNA transcripts of about 0. 9 and 3.0 kb were expressed only in the liver. The analysis of the phylogenetic tree of rabbit and bovine MBPs and other collectins indicates that the loss of MBP gene occurred not only during hominoid evolution but also at some points after the separation of birds and mammals.
Kawai
T
T
Department of Food Microbiology, Osaka Prefectural Institute of Public Health, Higashinari, Japan.
Suzuki
Y
Y
Eda
S
S
Ohtani
K
K
Kase
T
T
Sakamoto
T
T
Uemura
H
H
Wakamiya
N
N
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002352:Carrier Proteins / Q000097:blood / Q000737:chemistry*; D003001:Cloning, Molecular; D037222:Collectins; D003167:Complement Activation / Q000502:physiology; D019143:Evolution, Molecular; D006384:Hemagglutination / Q000502:physiology; D008099:Liver / Q000737:chemistry; D008969:Molecular Sequence Data; D009975:Orthomyxoviridae / Q000378:metabolism; D010802:Phylogeny; D012333:RNA, Messenger / Q000032:analysis; D011817:Rabbits; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D017385:Sequence Homology
D002352:Carrier Proteins; D037222:Collectins; D012333:RNA, Messenger