C-terminal end of v-src protein interacts with peptide coded by gadd7/adapt15-like RNA in two-hybrid system.
The significant differences in the metastatic properties of hamster fibroblasts transformed by the Rous sarcoma virus (RSV) were associated with mutations in the v-src carboxy-terminal region. To identify the capacity of this region for protein-protein interaction the two-hybrid system was used. The cDNA clone (vseap1), producing the protein specifically bound with the v-src C-terminal part in yeast cells in vivo and in GST-fusion system in vitro was isolated. Vseap1 shared 68% of homology with stressful agents induced RNA-gadd7/adapt15. Two vseap1 specific messenger RNAs were identified: 0.9-kbp RNA expressed in all transformed cells and three times less in embryo fibroblasts; 3.1-kbp transcript was deleted in the cells with suppressed v-src activity and H2O2 resistance.
Mizenina
O
O
Institute of Carcinogenesis, Cancer Research Center, Moscow, Russia.
Yanushevich
Y
Y
Musatkina
E
E
Rodina
A
A
Camonis
J
J
Tavitian
A
A
Tatosyan
A
A
D000595:Amino Acid Sequence; D000818:Animals; D001358:Avian Sarcoma Viruses / Q000235:genetics; D001483:Base Sequence; D002352:Carrier Proteins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D006224:Cricetinae; D004249:DNA Damage / Q000235:genetics; D018076:DNA, Complementary / Q000235:genetics; D004926:Escherichia coli / Q000235:genetics; D015972:Gene Expression Regulation, Neoplastic / Q000235:genetics*; D047908:Intracellular Signaling Peptides and Proteins; D008969:Molecular Sequence Data; D009362:Neoplasm Metastasis / Q000235:genetics*; D015688:Oncogene Protein pp60(v-src) / Q000737:chemistry / Q000235:genetics*; D011485:Protein Binding; D014176:Protein Biosynthesis / Q000235:genetics; D011506:Proteins / Q000737:chemistry*; D012333:RNA, Messenger / Q000032:analysis / Q000235:genetics; D011993:Recombinant Fusion Proteins / Q000235:genetics; D012441:Saccharomyces cerevisiae / Q000235:genetics; D014170:Transformation, Genetic / Q000235:genetics; D014407:Tumor Cells, Cultured
D002352:Carrier Proteins; D018076:DNA, Complementary; D047908:Intracellular Signaling Peptides and Proteins; D011506:Proteins; D012333:RNA, Messenger; D011993:Recombinant Fusion Proteins; C110828:vseap1 protein, Mesocricetus auratus; D015688:Oncogene Protein pp60(v-src)