title: Longitudinal differentiation of chromosomes of Asellus aquaticus (Crust. Isop.) by in situ nick translation using restriction enzymes and DNase I.
abstract: Asellus aquaticus is an isopod crustacean whose chromosomes cannot be differentiated by G- or R-banding techniques. In this work, we have obtained a longitudinal differentiation of these chromosomes by in situ nick translation using restriction enzymes (HaeIII, DraI and BamHI) and DNase I digestions. The four nucleases, with different efficiencies, have produced similar labelling patterns. Staining with DAPI, Giemsa and chromomycin A3 reveals that the DNA of the nick-translated regions is generally more resistant to extraction from the chromosome. The results obtained on the heteromorphic sex chromosome pair observed in about a quarter of the males of a natural population allow several hypotheses to be advanced on the nature and origin of chromosome dimorphism.
authors.affiliation: Dip. Genetica e Biol. Mol., Università La Sapienza, Rome, Italy.
authors.forename: R
authors.initials: R
authors.lastname: Barzotti
authors.forename: F
authors.initials: F
authors.lastname: Pelliccia
authors.forename: A
authors.initials: A
authors.lastname: Rocchi
mesh_terms: D000818:Animals; D002871:Chromosome Banding / Q000379:methods*; D003445:Crustacea / Q000235:genetics*; D003850:Deoxyribonuclease I; D015252:Deoxyribonucleases, Type II Site-Specific; D007621:Karyotyping / Q000379:methods; D008297:Male; D013093:Spermatogonia; D014960:X Chromosome; D014998:Y Chromosome
chemical_list: D003850:Deoxyribonuclease I; D015252:Deoxyribonucleases, Type II Site-Specific