Cytokine production in cell culture by peripheral blood mononuclear cells from immunocompetent hosts.
The production of interleukin 2 (IL-2) gamma interferon, IL-4, tumor necrosis factor alpha (TNF-alpha), TNF-beta, IL-5, and IL-10 in vitro by peripheral blood mononuclear cells cultured from healthy immunocompetent subjects after mitogen stimulation was determined. The mitogens used were concanavalin A, phytohemagglutinin, pokeweed mitogen, and Staphylococcus aureus Cowen. The results obtained provide a normal range for the production of these cytokines under specified conditions in vitro.
Katial
R K
RK
Department of Allergy and Immunology, Walter Reed Army Medical Center, Washington, DC 20307, USA. RKatial@POL.NET
Sachanandani
D
D
Pinney
C
C
Lieberman
M M
MM
D000328:Adult; D002478:Cells, Cultured / Q000737:chemistry / Q000187:drug effects; D003208:Concanavalin A / Q000494:pharmacology; D016207:Cytokines / Q000096:biosynthesis* / Q000097:blood / Q000187:drug effects; D005260:Female; D006801:Humans; D007121:Immunocompetence / Q000502:physiology; D007371:Interferon-gamma / Q000096:biosynthesis / Q000097:blood / Q000187:drug effects; D007378:Interleukins / Q000096:biosynthesis / Q000097:blood; D007963:Leukocytes, Mononuclear / Q000737:chemistry / Q000187:drug effects / Q000378:metabolism*; D008297:Male; D008875:Middle Aged; D008934:Mitogens / Q000494:pharmacology; D010835:Phytohemagglutinins / Q000494:pharmacology; D011043:Pokeweed Mitogens / Q000494:pharmacology; D012016:Reference Values; D014409:Tumor Necrosis Factor-alpha / Q000032:analysis / Q000096:biosynthesis / Q000187:drug effects
D016207:Cytokines; D007378:Interleukins; D008934:Mitogens; D010835:Phytohemagglutinins; D011043:Pokeweed Mitogens; D014409:Tumor Necrosis Factor-alpha; D003208:Concanavalin A; D007371:Interferon-gamma