Rapid diagnosis of chronic myeloid leukaemia by linking PCR to capillary gel electrophoresis.
The move to the use of molecular diagnostics in medicine has gathered momentum in the last few years and new methodologies are being sought to reduce the labour component and hence the cost of these molecular diagnostics. A method is described which links PCR to capillary electrophoresis and this allows both a rapid and quantitative diagnosis. In the example provided, the diagnosis of chronic myeloid leukaemia, the method describes improvements in cost per test, greater sensitivity over traditional methods and an estimate of the level of product. The latter points are important in those disorders for which bone marrow transplantation is used as a curative regime in providing earlier detection of relapse.
Kearney
P P
PP
Haematology Department, St Vincent's Hospital, Darlinghurst, New South Wales, Australia.
Aumatell
A
A
D003363:Cost Control; D019075:Electrophoresis, Capillary / Q000191:economics / Q000379:methods*; D004591:Electrophoresis, Polyacrylamide Gel / Q000191:economics / Q000379:methods*; D005453:Fluorescence; D006801:Humans; D007834:Lasers; D015464:Leukemia, Myelogenous, Chronic, BCR-ABL Positive / Q000175:diagnosis* / Q000191:economics; D016133:Polymerase Chain Reaction / Q000191:economics / Q000379:methods*; D012194:RNA-Directed DNA Polymerase; D012680:Sensitivity and Specificity; D013997:Time Factors
D012194:RNA-Directed DNA Polymerase