Porcine Dax-1 gene: isolation and expression during gonadal development.
The identification of XY females carrying a duplication of a region of the X chromosome (Xp21) led to the hypothesis that a double dose of a gene in the duplicated region causes sex reversal (DSS; dosage sensitive sex reversal). A gene isolated from this region, named DAX-1 (DSS-AHC critical region on the X), encodes a new member of the nuclear hormone receptor family. Here, we describe the isolation of porcine Dax-1 and the analysis of its pattern of expression both during foetal development and in several adult tissues. Dax-1 is expressed in the adrenals, the pituitary gland and the gonads at various stages of differentiation. In gonads, Dax-1 expression starts between 21 and 23 days post coitum in both XX and XY urogenital ridges then continues to be expressed until adult age. The expression in these tissues indicates the involvement of DAX-1 in the development and the function of the reproductive system at multiple levels.
Parma
P
P
Laboratoire de Biologie Cellulaire et Moléculaire, INRA, Jouy en Josas, France.
Pailhoux
E
E
Puissant
C
C
Cotinot
C
C
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D015152:Blotting, Northern; D015139:Blotting, Southern; D057137:DAX-1 Orphan Nuclear Receptor; D004247:DNA / Q000737:chemistry / Q000302:isolation & purification*; D004268:DNA-Binding Proteins / Q000737:chemistry / Q000235:genetics*; D005260:Female; D015870:Gene Expression; D005865:Gestational Age; D006066:Gonads / Q000196:embryology / Q000254:growth & development* / Q000378:metabolism*; D008297:Male; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000032:analysis; D018168:Receptors, Retinoic Acid / Q000737:chemistry / Q000235:genetics*; D012097:Repressor Proteins; D013552:Swine; D014157:Transcription Factors / Q000737:chemistry / Q000235:genetics*; D014960:X Chromosome
D057137:DAX-1 Orphan Nuclear Receptor; D004268:DNA-Binding Proteins; D012333:RNA, Messenger; D018168:Receptors, Retinoic Acid; D012097:Repressor Proteins; D014157:Transcription Factors; D004247:DNA