Localization by fluorescence in situ hybridization (FISH) of human mitochondrial polymerase gamma (POLG) to human chromosome band 15q24-->q26, and of mouse mitochondrial polymerase gamma (Polg) to mouse chromosome band 7E, with confirmation by direct sequence analysis of bacterial artificial chromosomes (BACs).
Cloned cDNAs for the human mitochondrial DNA polymerase gamma (POLG) were identified by homology with the yeast mitochondrial DNA polymerase catalytic subunit (MIP). Fluorescence in situ hybridization (FISH) of human and mouse bacterial artificial chromosomes (BACs), hybridized by radioactively labeled POLG cDNAs, mapped to human chromosome band 15q24-->q26, as well as to mouse chromosome band 7E. Direct sequencing of the BAC DNA without subcloning confirmed the presence of both human POLG and mouse mitochondrial DNA polymerase gamma (Polg) in the respective BACs.
Zullo
S J
SJ
Laboratory of Biochemical Genetics, National Institute of Mental Health, National Institutes of Health, Bethesda, MD 20892, USA. zullo@helix.nih.gov
Butler
L
L
Zahorchak
R J
RJ
Macville
M
M
Wilkes
C
C
Merril
C R
CR
D000818:Animals; D001483:Base Sequence; D002876:Chromosomes, Bacterial; D002884:Chromosomes, Human, Pair 15; D003001:Cloning, Molecular / Q000379:methods; D000074002:DNA Polymerase gamma; D004259:DNA-Directed DNA Polymerase / Q000235:genetics*; D018014:Gene Transfer Techniques; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D051379:Mice; D008928:Mitochondria / Q000201:enzymology / Q000235:genetics*; D008969:Molecular Sequence Data; D017422:Sequence Analysis, DNA; D013045:Species Specificity
D000074002:DNA Polymerase gamma; D004259:DNA-Directed DNA Polymerase