title: The effects of cryopreservation on the morphometric dimensions of caprine sperm heads.
abstract: Cryopreserved semen has been utilised in the artificial insemination of livestock species for over 40 years, even though the detrimental effects of cryopreservation on sperm function and fertility are well documented. In the present study, computer-automated sperm-head morphometry was used to determine if goat sperm-head morphometry was affected by freezing and thawing. A microscope slide was prepared from single semen samples, collected by artificial vagina, from 10 sexually active Saanen bucks. The remainder of each sample was frozen in a tris-citrate-yolk extender. After thawing, semen smears were prepared on microscope slides. All slides were stained in haematoxylin and mean sperm-head measurements of length, width, width/length, area and perimeter were determined for each slide by computer aided sperm morphometry analysis. The effects of sperm freezing on sperm-head dimensions within and among all bucks were determined. No significant (P > 0.10) freezing effect was found between fresh semen and postthaw samples for length (7.00 microns vs 7.13 microns), width (3.77 microns vs 3.87 microns), width/length (0.54 micron vs 0.54 micron), area (19.67 microns2 vs 20.57 microns2) and perimeter (18.62 microns vs 18.83 microns) when analysed across all bucks. Significant differences (P < 0.05) were however found within three bucks for area, perimeter, length and width, with the percentage increase in measurements being significantly greater than in the remaining bucks. The variability of the morphometric dimensions were not affected by freezing. The results indicate that semen freezing did not affect the overall dimensions of sperm heads across the entire population of bucks sampled. However, since sperm-head dimensions from three bucks were affected, changes in sperm-head morphometry may be indicative of spermatozoa of the semen from individuals to successfully freeze. Because the overall mean sperm-head dimensions acquired from frozen/thawed semen were not different from those of fresh semen, previously reported measurements of goat sperm heads are probably reflective of fresh semen. More importantly, retrospective studies of sperm-head morphometry and fertility may now be performed utilising extensive breeding records from frozen semen.
authors.affiliation: Department of Obstetrics and Gynaecology, University of Auckland, New Zealand. cggravance@ucdavis.edu
authors.forename: C G
authors.initials: CG
authors.lastname: Gravance
authors.forename: C
authors.initials: C
authors.lastname: White
authors.forename: K R
authors.initials: KR
authors.lastname: Robertson
authors.forename: Z J
authors.initials: ZJ
authors.lastname: Champion
authors.forename: P J
authors.initials: PJ
authors.lastname: Casey
mesh_terms: D000818:Animals; D015925:Cryopreservation / Q000662:veterinary*; D006041:Goats / Q000502:physiology*; D006651:Histocytochemistry; D007091:Image Processing, Computer-Assisted; D007315:Insemination, Artificial / Q000662:veterinary; D008297:Male; D012661:Semen / Q000502:physiology; D012662:Semen Preservation / Q000009:adverse effects / Q000662:veterinary*; D013077:Sperm Head / Q000502:physiology* / Q000648:ultrastructure*; D013081:Sperm Motility / Q000502:physiology