title: Mapping of the human genes encoding cyclin H (CCNH) and the CDK-activating kinase (CAK) assembly factor MAT1 (MNAT1) to chromosome bands 5q13.3-q14 and 14q23, respectively.
abstract: Cyclin-dependent kinases (CDKs), which play a key role in cell cycle control, are activated by the CDK-activating kinase (CAK), which activates cyclin-bound CDKs by phosphorylation at the specific threonine residue. Mammalian CAK contains three components: CDK7, cyclin H, and an assembly factor called MAT1. The CDK7-cyclin H-MAT1 complex is tightly associated with a multiprotein complex TFIIH, which plays a dual role in transcription and DNA repair. Here, we have determined chromosomal localizations of the human genes encoding cyclin H (CCNH) and MAT1 (HGMW-approved symbol MNAT1) to chromosome bands 5q13.3-q14 and 14q23, respectively, by using fluorescence in situ hybridization, somatic cell hybrid analyses, and mapping to the human YAC contigs.
authors.affiliation: Cellular Physiology Laboratory, Tsukuba Life Science Center, Ibaraki, Japan. eki@rtc.riken.go.jp
authors.forename: T
authors.initials: T
authors.lastname: Eki
authors.forename: K
authors.initials: K
authors.lastname: Okumura
authors.forename: M
authors.initials: M
authors.lastname: Abe
authors.forename: K
authors.initials: K
authors.lastname: Kagotani
authors.forename: H
authors.initials: H
authors.lastname: Taguchi
authors.forename: Y
authors.initials: Y
authors.lastname: Murakami
authors.forename: Z Q
authors.initials: ZQ
authors.lastname: Pan
authors.forename: F
authors.initials: F
authors.lastname: Hanaoka
mesh_terms: D000818:Animals; D001483:Base Sequence; D015139:Blotting, Southern; D002352:Carrier Proteins; D018797:Cell Cycle Proteins; D002874:Chromosome Mapping; D018244:Chromosomes, Artificial, Yeast; D002883:Chromosomes, Human, Pair 14; D002895:Chromosomes, Human, Pair 5; D006224:Cricetinae; D056748:Cyclin H; D018844:Cyclin-Dependent Kinases; D016213:Cyclins / Q000235:genetics*; D005819:Genetic Markers; D006801:Humans; D006822:Hybrid Cells; D017404:In Situ Hybridization, Fluorescence; D051379:Mice; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D017346:Protein Serine-Threonine Kinases / Q000235:genetics*; D014157:Transcription Factors; D000095043:Cyclin-Dependent Kinase-Activating Kinase
chemical_list: C534751:CCNH protein, human; D002352:Carrier Proteins; C534752:Ccnh protein, mouse; D018797:Cell Cycle Proteins; D056748:Cyclin H; D016213:Cyclins; D005819:Genetic Markers; C514215:MNAT1 protein, human; D014157:Transcription Factors; D017346:Protein Serine-Threonine Kinases; D018844:Cyclin-Dependent Kinases; D000095043:Cyclin-Dependent Kinase-Activating Kinase