title: Mediated electrochemistry of peroxidases--effects of variations in protein and mediator structures.
abstract: The kinetics of a range of ferrocene derivatives with horseradish peroxidase (HRP), cytochrome c peroxidase (CCP) and 3 charge reversal mutants of cytochrome c peroxidase were measured using cyclic voltammetry. Substantial differences in rate constant (100 fold) were observed between HRP and CCP for the same mediator with smaller differences (4-5 fold) for different mediators with the same enzyme. The rate constant did not seem to be dependent on redox potential differences. Cluster analysis is proposed as a way of classifying mediator reactivity.
authors.affiliation: Department of Biochemistry, Imperial College of Science, Technology and Medicine, London, UK.
authors.forename: S J
authors.initials: SJ
authors.lastname: Sadeghi
authors.forename: G
authors.initials: G
authors.lastname: Gilardi
authors.forename: A E
authors.initials: AE
authors.lastname: Cass
mesh_terms: D003578:Cytochrome-c Peroxidase / Q000737:chemistry*; D004563:Electrochemistry; D005296:Ferrous Compounds / Q000737:chemistry; D006735:Horseradish Peroxidase / Q000737:chemistry*; D000075163:Metallocenes; D010084:Oxidation-Reduction; D013329:Structure-Activity Relationship
chemical_list: D005296:Ferrous Compounds; D000075163:Metallocenes; D006735:Horseradish Peroxidase; D003578:Cytochrome-c Peroxidase; C004998:ferrocene