125I labelling of human serum albumin and fibrinogen and a study of protein adsorption properties on the surface of titanium oxide film.
In order to detect the surface concentration of proteins adsorbed on a solid surface for selecting blood compatible materials, a gentle iodination reagent, Iodogen, was used to label human serum albumin and fibrinogen, and has been applied to the study of protein adsorption properties on a plate of titanium oxide film. The yields of the labelled albumin and fibrinogen are 69.7% and 49.6%. The results of adsorption show that [125I]HSA and [125I]HFG are efficacious at the surface concentration detection and can be used to investigate the protein adsorption properties of a solid material.
Liu
F
F
Shanghai Institute of Nuclear Research, Academia Sinica, Department of RIA, P. R. China.
Zhou
M
M
Zhang
F
F
D000327:Adsorption; D001672:Biocompatible Materials / Q000737:chemistry*; D005340:Fibrinogen / Q000737:chemistry*; D006801:Humans; D007457:Iodine Radioisotopes / Q000737:chemistry*; D007553:Isotope Labeling; D007700:Kinetics; D012709:Serum Albumin / Q000737:chemistry*; D013499:Surface Properties; D013816:Thermodynamics; D014025:Titanium / Q000737:chemistry*
D001672:Biocompatible Materials; D007457:Iodine Radioisotopes; D012709:Serum Albumin; C009495:titanium dioxide; D005340:Fibrinogen; D014025:Titanium