title: Sequence analysis of the groESL-cotA region of the Bacillus subtilis genome, containing the restriction/modification system genes.
abstract: We have determined a 35-kb sequence of the groESL-gutR-cotA (45 degrees-52 degrees) region of the Bacillus subtilis genome. In addition to the groESL, gutRB and cotA genes reported previously, we have newly identified 24 ORFs including gutA and fruC genes, encoding glucitol permease and fructokinase, respectively. The inherent restriction/modification system genes, hsdMR and hsdMM, were mapped between groESL and gutRB, and we have identified two open reading frames (ORFs) encoding 5-methylcytosine forming DNA methyl transferase and an operon probably encoding a restriction enzyme complex. The unusual genome structure of few ORFs and lower GC content around the restriction/modification genes strongly suggests that the region originated from a bacteriophage integrated during evolution.
authors.affiliation: Graduate School of Biological Sciences, Nara Institute of Science and Technology, Japan. ysadaie@lab.nig.ac.jp
authors.forename: Y
authors.initials: Y
authors.lastname: Kasahara
authors.forename: S
authors.initials: S
authors.lastname: Nakai
authors.forename: N
authors.initials: N
authors.lastname: Ogasawara
authors.forename: K
authors.initials: K
authors.lastname: Yata
authors.forename: Y
authors.initials: Y
authors.lastname: Sadaie
mesh_terms: D000595:Amino Acid Sequence; D001412:Bacillus subtilis / Q000737:chemistry* / Q000235:genetics*; D001483:Base Sequence; D015280:DNA Restriction-Modification Enzymes / Q000737:chemistry* / Q000235:genetics*; D004269:DNA, Bacterial / Q000737:chemistry; D004587:Electrophoresis, Agar Gel; D016680:Genome, Bacterial; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D016133:Polymerase Chain Reaction; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D013012:Sorbitol / Q000378:metabolism
chemical_list: D015280:DNA Restriction-Modification Enzymes; D004269:DNA, Bacterial; D013012:Sorbitol