Identification and characterization of a protein kinase-interacting protein encoded by the Autographa californica nuclear polyhedrosis virus.
We have used a yeast two-hybrid system to identify a baculoviral gene encoding a protein kinase-interacting protein (PKIP). The pkip gene is located at 15.8-16.2 m.u (map units) and represents ORF 24 in the sequence of Ayres et al. (1994) of the Autographa californica nuclear polyhedrosis virus. PKIP is a 19.2-kDa protein and appears to stimulate the activity of the viral protein kinase-1 in vitro. Northern blotting analysis revealed that pkip is a late gene. Attempts to plaque purify a pkip- virus were unsuccessful.
Fan
X
X
Department of Biochemistry, Cell and Molecular Biology, University of Kansas, Lawrence 66045-2106, USA.
McLachlin
J R
JR
Weaver
R F
RF
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D015152:Blotting, Northern; D002352:Carrier Proteins / Q000737:chemistry* / Q000302:isolation & purification*; D004789:Enzyme Activation; D005822:Genetic Vectors; D008969:Molecular Sequence Data; D017924:Nucleopolyhedroviruses / Q000235:genetics*; D016415:Sequence Alignment; D018411:Spodoptera / Q000821:virology*; D014764:Viral Proteins / Q000378:metabolism*; D015003:Yeasts / Q000235:genetics
D002352:Carrier Proteins; D014764:Viral Proteins; C110371:pkip protein, Autographa californica nucleopolyhedrovirus