title: A comparison of ONTRAK TESTCUP, abuscreen ONTRAK, abuscreen ONLINE, and GC/MS urinalysis test results.
abstract: This study was designed to compare results obtained from two separate on-site drug testing kits (ONTRAK TESTCUP and Abuscreen ONTRAK) with those obtained from laboratory based immunoassay and GC/MS. Abuscreen ONLINE immunoassay was used to select 250 negative samples and 100 presumptive-positive samples each for cocaine/metabolites, opiates and cannabinoids. Presumptive-positive samples were selected if the immunoassay response was > or = 300 ng/mL for cocaine/metabolites (BZE), > or = 300 ng/mL for opiates or > or = 50 ng/mL for cannabinoids (THC-COOH). GC/MS was used to confirm that each selected sample contained > or = 150 ng/mL BZE, > or = 300 ng/mL morphine/codeine or > or = ng/mL THC-COOH. TESTCUP results had a 100% agreement with GC/MS and a > 99% agreement with ONLINE when testing negative samples. The agreement between TESTCUP and ONLINE results for samples containing opiates was 100%. Results of testing samples containing BZE with TESTCUP demonstrated a 98% agreement with both GC/MS and ONLINE. Both discrepant samples contained BZE at concentrations < or = 300 ng/mL. The least agreement between TESTCUP and ONLINE results was found when testing samples containing THC-COOH. The agreement with ONLINE and GC/MS was 92% and all discrepant samples had GC/MS determined THC-COOH concentrations less than 50 ng/mL. A 100% agreement was obtained between expected and recorded TESTCUP results for QC samples fortified to contained BZE, morphine or THC-COOH at concentrations within 120% of the screening cutoffs. ONTRAK had a 100% agreement with both GC/MS and ONLINE when testing negative samples and samples that contained opiates. ONTRAK had a 91% agreement with GC/MS and ONLINE for testing of samples that contained BZE. The least agreement between ONTRAK and ONLINE results was found when testing samples that contained THC-COOH. The agreement was 89%, however, all discrepant samples contained GC/MS concentrations of THC-COOH less that the 50 ng/mL cutoff. With ONTRAK, a 100% agreement was obtained between expected and recorded results QC samples that contained morphine or THC-COOH and a 97.7% agreement was obtained between expected and recorded results on QC samples that contained BZE.
authors.affiliation: Center for Human Toxicology, University of Utah, Salt Lake City, USA.
authors.forename: D J
authors.initials: DJ
authors.lastname: Crouch
authors.forename: M L
authors.initials: ML
authors.lastname: Cheever
authors.forename: D M
authors.initials: DM
authors.lastname: Andrenyak
authors.forename: D J
authors.initials: DJ
authors.lastname: Kuntz
authors.forename: D L
authors.initials: DL
authors.lastname: Loughmiller
mesh_terms: D002186:Cannabinoids / Q000652:urine; D003042:Cocaine / Q000652:urine; D005554:Forensic Medicine / Q000379:methods; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods / Q000592:standards*; D006801:Humans; D007118:Immunoassay / Q000379:methods / Q000592:standards*; D009020:Morphine / Q000652:urine; D009294:Narcotics / Q000652:urine; D011786:Quality Control; D012680:Sensitivity and Specificity; D015813:Substance Abuse Detection / Q000379:methods / Q000592:standards*; D014116:Toxicology / Q000379:methods; D016482:Urinalysis / Q000295:instrumentation / Q000379:methods* / Q000592:standards
chemical_list: D002186:Cannabinoids; D009294:Narcotics; D009020:Morphine; D003042:Cocaine