title: Genotypic mapping of HPV and assessment of EBV prevalence in endocervical lesions.
abstract: OBJECTIVE
  To examine the prevalence of human papillomavirus (HPV) and Epstein-Barr virus (EBV) in low grade glandular intraepithelial lesions of the cervix, adenocarcinoma with high grade glandular intraepithelial lesions combined, and adenocarcinomas; and to perform a genotyping mapping analysis of endocervical carcinomas to determine the extent of HPV infections in such lesions.
  
  
  METHODS
  Archival paraffin wax embeded material from the files of the departments of pathology, National Maternity Hospital, Dublin, and University College Cork, Ireland.
  
  
  METHODS
  HPV prevalence was examined using type specific HPV PCR, general primer HPV PCR (pan HPV screen), nonisotopic in situ hybridisation (NISH), and PCR in situ hybridisation (PCR-ISH). In situ hybridisation was performed using fluorescein labelled oligonucleotide cocktail for eber transcripts of EBV. Genotypic analysis was performed, in all cases where possible, using a grid system.
  
  
  RESULTS
  HPV 16 and 18 were predominantly identified in low grade glandular intraepithelial lesions, high grade glandular intraepithelial lesions, and adenocarcinomas, with HPV prevalence increasing with grade of dysplasia. EBV was only identified in subepithelial lymphocytes in a minority of cases. No link could be shown between HPV and EBV in endocervical lesions. HPV infection was not clonal in endocervical cancer and coexistent adjacent cervical intraepithelial neoplasia, where present, tended to show a similar HPV type.
  
  
  CONCLUSIONS
  The restriction of HPV types 16 and 18 to endocervical lesions suggests that their effect is restricted and specific to endocervical mucosa, but the mechanism of interaction is currently unknown.
authors.affiliation: Nuffield Department of Pathology and Bacteriology, University of Oxford, UK.
authors.forename: J J
authors.initials: JJ
authors.lastname: O'Leary
authors.forename: R J
authors.initials: RJ
authors.lastname: Landers
authors.forename: M
authors.initials: M
authors.lastname: Crowley
authors.forename: I
authors.initials: I
authors.lastname: Healy
authors.forename: W F
authors.initials: WF
authors.lastname: Kealy
authors.forename: J
authors.initials: J
authors.lastname: Hogan
authors.forename: C
authors.initials: C
authors.lastname: Cullinane
authors.forename: P
authors.initials: P
authors.lastname: Kelehan
authors.forename: C T
authors.initials: CT
authors.lastname: Doyle
mesh_terms: D000230:Adenocarcinoma / Q000821:virology*; D000328:Adult; D005260:Female; D005838:Genotype; D004854:Herpesvirus 4, Human / Q000302:isolation & purification*; D006801:Humans; D017403:In Situ Hybridization; D027383:Papillomaviridae / Q000145:classification / Q000302:isolation & purification*; D030361:Papillomavirus Infections / Q000150:complications; D016133:Polymerase Chain Reaction; D014412:Tumor Virus Infections / Q000150:complications; D002583:Uterine Cervical Neoplasms / Q000473:pathology / Q000821:virology*; D002578:Uterine Cervical Dysplasia / Q000473:pathology / Q000821:virology*