Role of iron and iron chelation therapy in oxygen free radical mediated tissue injury in an ascending mouse model of chronic pyelonephritis.
The contribution of iron towards the free radical generation leading to renal tissue damage was assessed using a non-obstructive ascending mouse model for chronic pyelonephritis. The parameters studied include luminol dependent chemiluminescence (LDCL), histopathology and some biochemical investigations. We found that iron enhanced the renal tissue damage and led to renal scarring, and end point in chronic renal inflammation, irrespective of the bacterial strain studied. In addition a role of iron chelation therapy as a treatment for chronic renal inflammation is also suggested.
Gupta
R
R
Government Medical College, Department of Microbiology, Chandigarh, India.
Gupta
S
S
Joshi
K
K
Ganguly
N K
NK
D000818:Animals; D015913:Chelation Therapy / Q000009:adverse effects*; D002908:Chronic Disease; D019343:Citric Acid / Q000009:adverse effects*; D003676:Deferoxamine / Q000009:adverse effects*; D004195:Disease Models, Animal; D004338:Drug Combinations; D004926:Escherichia coli / Q000502:physiology; D004927:Escherichia coli Infections / Q000139:chemically induced / Q000473:pathology / Q000503:physiopathology*; D005260:Female; D005290:Ferric Compounds / Q000009:adverse effects*; D005609:Free Radicals / Q000494:pharmacology; D005954:Glucosephosphate Dehydrogenase / Q000032:analysis; D005980:Glutathione Reductase / Q000032:analysis; D006651:Histocytochemistry; D007501:Iron; D007668:Kidney / Q000187:drug effects / Q000473:pathology* / Q000503:physiopathology; D007770:L-Lactate Dehydrogenase / Q000032:analysis; D008054:Lipid Peroxides / Q000032:analysis; D008163:Luminescent Measurements; D051379:Mice; D008807:Mice, Inbred BALB C; D011704:Pyelonephritis / Q000139:chemically induced / Q000473:pathology / Q000503:physiopathology*; D017382:Reactive Oxygen Species / Q000502:physiology*; D013012:Sorbitol / Q000009:adverse effects*
D004338:Drug Combinations; D005290:Ferric Compounds; D005609:Free Radicals; D008054:Lipid Peroxides; D017382:Reactive Oxygen Species; C008170:citric acid, iron, sorbitol drug combination; D019343:Citric Acid; D013012:Sorbitol; D007501:Iron; D007770:L-Lactate Dehydrogenase; D005954:Glucosephosphate Dehydrogenase; D005980:Glutathione Reductase; D003676:Deferoxamine