The major core protein of messenger ribonucleoprotein particles (p50) promotes initiation of protein biosynthesis in vitro.
The major core protein of cytoplasmic messenger ribonucleoprotein particles (p50) has been shown previously to inhibit protein synthesis in vitro and in vivo. Furthermore, p50 is highly homologous to the Y-box-binding transcription factor family of proteins, binds DNA containing the Y-box motif, and thus may have a dual function in cells as a regulator of both transcription and translation. Here we show that binding or removal of p50 from rabbit reticulocyte lysate by monospecific antibodies to p50 strongly inhibits translation of endogenous and exogenous globin mRNAs as well as prokaryotic beta-galactosidase mRNA in a rabbit reticulocyte cell-free system. Thus, depending on the conditions, p50 not only may act as a translational repressor, but may also be required for protein synthesis. Translation inhibition with anti-p50 antibodies is not a result of mRNA degradation or its functional inactivation. The inhibition does not change the ribosome transit time, and therefore, it does not affect elongation/termination of polypeptide chains. The inhibition with anti-p50 antibodies is followed by a decay of polysomes and accumulation of the 48 S preinitiation complex. These results suggest that p50 participates in initiation of protein biosynthesis. Although uninvolved in the formation of the 48 S preinitiation complex, p50 is necessary either for attachment of the 60 S ribosomal subunit or for previous 5'-untranslated region scanning by the 43 S preinitiation complex.
Evdokimova
V M
VM
Institute of Protein Research, Russian Academy of Sciences, Pushchino, Moscow Region 142292, Russian Federation.
Kovrigina
E A
EA
Nashchekin
D V
DV
Davydova
E K
EK
Hershey
J W
JW
Ovchinnikov
L P
LP
D000818:Animals; D002474:Cell-Free System; D007074:Immunoglobulin G / Q000276:immunology; D014176:Protein Biosynthesis; D011506:Proteins / Q000235:genetics; D011817:Rabbits; D012261:Ribonucleoproteins / Q000378:metabolism*; D001616:beta-Galactosidase / Q000037:antagonists & inhibitors / Q000096:biosynthesis
D007074:Immunoglobulin G; D011506:Proteins; D012261:Ribonucleoproteins; C026452:messenger ribonucleoprotein; D001616:beta-Galactosidase