Intragenic suppressors of induction-deficient TetR mutants: localization and potential mechanism of action.
Eight Tn10 Tet repressor mutants with an induction-deficient phenotype and with primary mutations located at or close to the dimer interface were mutagenized and screened for inducibility in the presence of tetracycline. The second-site suppressors with wild-type-like operator binding activity that were obtained act, except for one, at a distance, suggesting that they contribute to conformational changes in the Tet repressor. Many of these long-range suppressors occur along the dimer interface, indicating that interactions between the monomers play an important role in Tet repressor induction.
Biburger
M
M
Lehrstuhl für Mikrobiologie, Institut für Mikrobiologie, Biochemie und Genetik der Friedrich-Alexander Universität Erlangen-Nürnberg, Erlangen, Germany.
Berens
C
C
Lederer
T
T
Krec
T
T
Hillen
W
W
D018360:Crystallography, X-Ray; D004251:DNA Transposable Elements; D019281:Dimerization; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism; D016296:Mutagenesis; D009875:Operator Regions, Genetic; D011487:Protein Conformation; D012097:Repressor Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism*; D013329:Structure-Activity Relationship; D013489:Suppression, Genetic; D013752:Tetracycline / Q000494:pharmacology
D004251:DNA Transposable Elements; D012097:Repressor Proteins; C034811:tetracycline resistance-encoding transposon repressor protein; D013752:Tetracycline