title: In vivo detection of calcium from dicalcium phosphate dihydrate dentifrices in demineralized human enamel and plaque.
abstract: A series of studies was conducted to clarify the role of DCPD in improving the effects of fluoride in the mouth. We performed plaque fluid studies to see if the use of DCPD dentifrices increases the levels of free Ca2+ ions and the DSenamel in plaque fluid. The results of these studies showed that plaque fluid was undersaturated with respect to DCPD; therefore, DCPD would dissolve in the mouth. The Ca2+ activity in plaque fluid measured 12 hours post-treatment was significantly elevated with the use of DCPD dentifrices in comparison with silica dentifrices. The use of DCPD dentifrices also increased the DSenamel of plaque fluid in comparison with a NaF/silica dentifrice. To determine whether Ca from DCPD could be detected in demineralized enamel and whole plaque after in vivo usage of a DCPD/ fluoride dentifrice, we conducted a 44Ca labeling study. Secondary Ion Mass Spectrometry (SIMS) was used to detect and measure changes in 44Ca levels in enamel before and after 6 days of in vivo treatment with a 44DCPD/fluoride dentifrice. The results showed that 44Ca from DCPD was incorporated into enamel and detected in plaque 18 hours post-treatment. The findings from these studies show that brushing with a DCPD dentifrice introduces additional, exogenous Ca into the oral environment, which fosters improved remineralization of teeth in combination with fluoride.
authors.affiliation: Colgate-Palmolive Company, Piscataway, New Jersey 08854, USA.
authors.forename: R J
authors.initials: RJ
authors.lastname: Sullivan
authors.forename: A
authors.initials: A
authors.lastname: Charig
authors.forename: J
authors.initials: J
authors.lastname: Blake-Haskins
authors.forename: Y P
authors.initials: YP
authors.lastname: Zhang
authors.forename: S M
authors.initials: SM
authors.lastname: Miller
authors.forename: M
authors.initials: M
authors.lastname: Strannick
authors.forename: A
authors.initials: A
authors.lastname: Gaffar
authors.forename: H C
authors.initials: HC
authors.lastname: Margolis
mesh_terms: D002118:Calcium / Q000032:analysis*; D002130:Calcium Phosphates / Q000627:therapeutic use*; D002132:Calcium Radioisotopes; D018592:Cross-Over Studies; D003743:Dental Enamel / Q000737:chemistry*; D003773:Dental Plaque / Q000737:chemistry*; D003802:Dentifrices / Q000627:therapeutic use*; D005122:Exudates and Transudates / Q000737:chemistry; D005459:Fluorides / Q000627:therapeutic use; D006801:Humans; D012822:Silicon Dioxide / Q000627:therapeutic use; D018629:Spectrometry, Mass, Secondary Ion; D013997:Time Factors; D017001:Tooth Demineralization / Q000378:metabolism* / Q000628:therapy; D014100:Toothpastes / Q000627:therapeutic use
chemical_list: D002130:Calcium Phosphates; D002132:Calcium Radioisotopes; D003802:Dentifrices; D014100:Toothpastes; C485828:alpha-tricalcium phosphate; C485830:tetracalcium phosphate; C485838:calcium phosphate, monobasic, anhydrous; D012822:Silicon Dioxide; C020243:calcium phosphate; C485829:calcium phosphate, dibasic, anhydrous; D005459:Fluorides; D002118:Calcium