GnRH molecular variants in the brain and pituitary gland of pejerrey, Odontesthes bonariensis (Atheriniformes). Immunological and chromatographic evidence for the presence of a novel molecular variant.
Gonadotropin-releasing hormone (GnRH) molecular variants in the brain and pituitary gland of pejerrey, Odontesthes bonariensis (Atheriniformes), were characterized by gradient reverse phase high performance liquid chromatography (RP-HPLC). Eluted fractions were tested in radioimmunoassays with different antisera. The results show that the brain extract contains three forms of GnRH: one is immunologically and chromatographically similar to cIIGnRH (chicken II), and another is similar to sGnRH (salmon). A third GnRH appears to be chromatographic and immunologically different from the nine other known forms of the vertebrate hormone. This is the only variant present in the pituitary gland.
Stefano
A V
AV
Instituto de Neurociencia (INEUCI-CONICET), Buenos Aires, Argentina.
Canosa
L F
LF
D'Eramo
J L
JL
Fridman
O
O
Affanni
J M
JM
Somoza
G M
GM
D000818:Animals; D001118:Argentina; D001921:Brain / Q000378:metabolism*; D001923:Brain Chemistry; D002851:Chromatography, High Pressure Liquid; D005399:Fishes; D007987:Gonadotropin-Releasing Hormone / Q000031:analogs & derivatives* / Q000032:analysis / Q000737:chemistry / Q000302:isolation & purification* / Q000378:metabolism; D010902:Pituitary Gland / Q000737:chemistry / Q000378:metabolism*; D011498:Protein Precursors / Q000032:analysis / Q000737:chemistry / Q000378:metabolism*; D011863:Radioimmunoassay; D014020:Tissue Extracts / Q000737:chemistry
D011498:Protein Precursors; D014020:Tissue Extracts; C091931:salmon gonadotropin-releasing hormone precursor; D007987:Gonadotropin-Releasing Hormone