title: In-vitro effect of estrogen-antagonist on motility and penetration ability of human spermatozoa.
abstract: Antiestrogens affect spermatozoa through their action on Leydig and Sertoli cells. Direct effect of antiestrogens namely tamoxifen and centchroman in concentration of 1, 2.5, 5, 10 and 20 micrograms/ml in incubation medium was determined on motility and penetration ability of human spermatozoa. Motility (%) was invariably reduced after 15, 30 and 60 min. of incubation. Addition of 17 beta-estradiol to medium with antagonist caused inhibition of motility in dose related manner. The distance travelled by spermatozoa treated with tamoxifen or centchroman in media was reduced by 30% and addition of estradiol along with antiestrogen reduced it to 50% compared to that of untreated spermatozoa.
authors.affiliation: Department of Reproductive Biomedicine, National Institute of Health & Family Welfare, Munirka, New Delhi, India.
authors.forename: I S
authors.initials: IS
authors.lastname: Allag
authors.forename: K
authors.initials: K
authors.lastname: Rangari
mesh_terms: D000328:Adult; D002486:Centchroman / Q000494:pharmacology*; D004958:Estradiol / Q000494:pharmacology*; D004965:Estrogen Antagonists / Q000494:pharmacology*; D005260:Female; D006801:Humans; D066298:In Vitro Techniques; D008297:Male; D013081:Sperm Motility / Q000187:drug effects*; D013084:Sperm-Ovum Interactions / Q000187:drug effects*; D013629:Tamoxifen / Q000494:pharmacology*
chemical_list: D004965:Estrogen Antagonists; D013629:Tamoxifen; D002486:Centchroman; D004958:Estradiol