Characterization of the egg vesicular components in the seaweed, Fucus serratus L. (Fucales, Phaeophyta), using enzyme histochemistry and vital staining: the search for a lysosome-like body.
Fucus serratus eggs were examined for evidence of the existence of a lysosome-like body using enzyme histochemical and vital staining techniques. Simultaneous coupling azo-dye techniques for lysosomal acid phosphatase proved inappropriate owing to endogenous phenolic binding artefacts. The large number of alginate polysaccharide and polyphenolic egg vesicles interfered with vital staining techniques for lysosomes. Lysosomal esterase activity was detected in the abundant egg lipid bodies. The role of the egg lipid body as an equivalent lysosome-like body of higher plants, the spherosome, is discussed in relation to egg fertilization and early zygote development.
Holland
R D
RD
Washington Singer Laboratories, Department of Biological Sciences, University of Exeter, Devon.
Pitt
D
D
Moore
M N
MN
Brownlee
C
C
D000135:Acid Phosphatase / Q000378:metabolism; D000165:Acridine Orange; D004396:Coloring Agents; D004950:Esterases / Q000378:metabolism; D005456:Fluorescent Dyes; D006651:Histocytochemistry; D008247:Lysosomes / Q000201:enzymology / Q000648:ultrastructure*; D009499:Neutral Red; D010078:Oxazines; D011134:Polysaccharides / Q000378:metabolism; D012624:Seaweed / Q000166:cytology* / Q000201:enzymology / Q000648:ultrastructure; D015053:Zygote / Q000201:enzymology / Q000648:ultrastructure*
D004396:Coloring Agents; D005456:Fluorescent Dyes; D010078:Oxazines; D011134:Polysaccharides; D009499:Neutral Red; D004950:Esterases; D000135:Acid Phosphatase; D000165:Acridine Orange; C044808:nile red