Modification of catalase by chondroitin sulfate.
Catalase was chemically modified by sodium chondroitin sulfate using the benzoquinone binding method. Thus, 40-42% of the catalase preparation was modified. Treatment of catalase and superoxide dismutase with benzoquinone-activated chondroitin sulfate results in a bienzymic conjugate with electrophoretically heterogenous composition. The yield of the products and their residual catalytic activity indicate that the method can be used for the preparation of modified catalase and the bienzymic conjugate to study their efficiency in vivo.
Maksimenko
A V
AV
Institute of Experimental Cardiology, Russian Cardiology Research Center, Moscow, Russia. csc@adonis.ias.msk.su
Tischenko
E G
EG
D016227:Benzoquinones / Q000378:metabolism; D002374:Catalase / Q000378:metabolism*; D002384:Catalysis; D002809:Chondroitin Sulfates / Q000378:metabolism*; D002850:Chromatography, Gel; D004591:Electrophoresis, Polyacrylamide Gel; D013482:Superoxide Dismutase / Q000378:metabolism
D016227:Benzoquinones; C004532:quinone; D002809:Chondroitin Sulfates; D002374:Catalase; D013482:Superoxide Dismutase