Molecular cloning and characterization of cDNAs for the mu-type large subunit and the small subunit of chicken calpain.
In chicken, three calpain isozymes expressed ubiquitously, mu-, mu/m- and m-types, have been identified at the cDNA and/or protein level, but the complete sequence of the mu-calpain large subunit (microCL) and its small subunit (30K) remain to be determined. In this report, we isolated and identified cDNA clones for chicken microCL and 30K, uncovering all molecules of the chicken ubiquitous calpain system. The longest open reading frame of microCL encodes 715 amino acid residues (M(r) = 81,410). The deduced amino acid sequence is more similar to that of human microCL (82%) than that of chicken mCL (61.6%) and mu/mCL (70.6%). As for 30K, several cDNA clones were isolated, but a full length cDNA are not isolated, presumably because of a GC-rich sequence of the 5'-terminus. The longest open reading frame encodes 214 amino acid residues, showing significant similarity to mammalian 30Ks. Northern blot analysis of microCL and 30K mRNA shows ubiquitous expression in all chicken tissues examined. The results indicate that microCL and 30K are well conserved in both chicken and mammal, confirming that at least three independent ubiquitous calpain species exist in chicken.
Jeong
S Y
SY
Department of Molecular Biology, University of Tokyo, Japan.
Sorimachi
H
H
Lee
H J
HJ
Ishiura
S
S
Suzuki
K
K
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002154:Calpain / Q000235:genetics*; D002645:Chickens; D003001:Cloning, Molecular; D018076:DNA, Complementary; D006801:Humans; D008969:Molecular Sequence Data; D012333:RNA, Messenger; D017421:Sequence Analysis; D017386:Sequence Homology, Amino Acid; D014018:Tissue Distribution
D018076:DNA, Complementary; D012333:RNA, Messenger; D002154:Calpain