Isolation of specific DNA probes for detection of Cryptococcus neoformans.
We report the construction of a genomic library and isolation of repetitive DNA probes from Cr. neoformans that are species- and variety-specific. The inserts of the genomic library were in a range of 0.3-1.8 kb, which were in normal distribution in length. Among 592 clones of the genomic library, there were 400(67.6%) clones inserted repetitive DNA sequence and 192(32.4%) inserted single copy DNA sequence of the Cr. neoformans genome. From the library, three specific probes were identified. Clone pCNIIA6 was serotype A-specific, pCNIIB5 species-specific, and pCNIIIG1 variety-specific (var. neoformans). On the basis of the high specificity and sensitivity of the signals observed by hybridization, we suggest that these probes provide a means for both biotyping and early diagnosis of Cr. neoformans.
Wu
J
J
Department of Dermatology, Shanghai Changzheng Hospital, P. R. China.
Liao
W
W
Chai
J
J
D003455:Cryptococcus neoformans / Q000235:genetics* / Q000302:isolation & purification*; D015342:DNA Probes / Q000737:chemistry / Q000302:isolation & purification*; D004271:DNA, Fungal / Q000302:isolation & purification*; D004274:DNA, Recombinant / Q000138:chemical synthesis; D004798:Enzymes; D015723:Gene Library; D005820:Genetic Testing; D006801:Humans; D017403:In Situ Hybridization; D010957:Plasmids / Q000138:chemical synthesis
D015342:DNA Probes; D004271:DNA, Fungal; D004274:DNA, Recombinant; D004798:Enzymes; C045154:novozym 234