An improved chromatographic method for the separation of egg yolk IgG into subpopulations utilizing immobilized metal ion (Fe3+) affinity chromatography.
An improved methodology is described for the separation of yolk IgG into subpopulations using immobilized metal ion (Fe3+) affinity chromatography. The yolk IgG was first extracted using a prechilled, pre-acidified method. After extraction, the yolk IgG was then fractionated using an Fe3+ column. Using an ascending pH gradient, four IgG containing peaks were well resolved based upon the elution pH, specific activity and the relative avidity index.
Greene
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U.S. Department of Agriculture, Agricultural Research Service, Southeast Poultry Research Laboratory, Athens, GA 30605, USA.
Holt
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D000818:Animals; D002645:Chickens; D002712:Chlorides; D002846:Chromatography, Affinity / Q000379:methods*; D004530:Egg Yolk / Q000276:immunology*; D005290:Ferric Compounds; D007098:Imino Acids; D007074:Immunoglobulin G / Q000302:isolation & purification*
D002712:Chlorides; D005290:Ferric Compounds; D007098:Imino Acids; D007074:Immunoglobulin G; C024555:ferric chloride