title: PyNPase expression in human colon cancer.
abstract: Although colorectal cancer tissue is rich in pyrimidine nucleoside phosphorylase (PyNPase), there is no consensus as to whether cancer cells or stromal cells predominately express PyNPase. We micro-dissected OCT compound embedded frozen tissue sections into epithelial and stromal components and then analyzed the extracted samples separately. The PyNPase expression level was higher in stromal cells than in cancer cells and the difference increased with inflammation induced by the immunostimulator OK432. These results suggest that stromal cells are the major PyNPase source in colorectal cancer.
authors.affiliation: Department of Surgery II, Osaka University Medical School, Suita, Japan.
authors.forename: A
authors.initials: A
authors.lastname: Haba
authors.forename: T
authors.initials: T
authors.lastname: Monden
authors.forename: M
authors.initials: M
authors.lastname: Sekimoto
authors.forename: K
authors.initials: K
authors.lastname: Ikeda
authors.forename: H
authors.initials: H
authors.lastname: Izawa
authors.forename: T
authors.initials: T
authors.lastname: Kanou
authors.forename: M
authors.initials: M
authors.lastname: Amano
authors.forename: H
authors.initials: H
authors.lastname: Kan'yama
authors.forename: M
authors.initials: M
authors.lastname: Monden
mesh_terms: D000328:Adult; D000368:Aged; D003110:Colonic Neoplasms / Q000201:enzymology*; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D008297:Male; D008875:Middle Aged; D010430:Pentosyltransferases / Q000378:metabolism*; D057845:Pyrimidine Phosphorylases; D017154:Stromal Cells / Q000201:enzymology
chemical_list: D010430:Pentosyltransferases; D057845:Pyrimidine Phosphorylases