title: [Enterohemorrhagic Escherichia coli O157 outbreak in Obihiro-city--biological and molecular characteristics among O157 isolates].
abstract: The outbreak of enterohaemorrhagic Escherichia coli (EHEC) O157:H7 infection in Obihiro City, Japan, occurred in late October 1996. The infection affected a total of 169 kindergarten pupils and school staff members in a private kindergarten. Twenty-one children (12.4%) progressed into hemolytic uremic syndrome (HUS). Moreover, the person-to-person infections in 9 families and the duration of excretion of EHEC in 13 patients were observed. The contaminated food was identified as the potato-salad served at lunch. Analysis of biological characteristics, the ability of toxin production, and the DNA analysis by PCR-based fingerprinting, the RAPD tests, among all clinical isolates, clarified a homologous origin of contamination.
authors.affiliation: Department of Veterinary Microbiology, Obihiro University of Agriculture and Veterinary Medicine.
authors.forename: S
authors.initials: S
authors.lastname: Makino
authors.forename: H
authors.initials: H
authors.lastname: Asakura
authors.forename: T
authors.initials: T
authors.lastname: Shirahata
authors.forename: T
authors.initials: T
authors.lastname: Ikeda
authors.forename: K
authors.initials: K
authors.lastname: Takeshi
authors.forename: H
authors.initials: H
authors.lastname: Nagano
authors.forename: S
authors.initials: S
authors.lastname: Yano
authors.forename: T
authors.initials: T
authors.lastname: Kubota
authors.forename: N
authors.initials: N
authors.lastname: Fujii
mesh_terms: D000328:Adult; D001427:Bacterial Toxins / Q000032:analysis; D002648:Child; D004196:Disease Outbreaks; D016521:Electrophoresis, Gel, Pulsed-Field; D004927:Escherichia coli Infections / Q000453:epidemiology* / Q000382:microbiology; D019453:Escherichia coli O157 / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism; D006463:Hemolytic-Uremic Syndrome / Q000453:epidemiology* / Q000382:microbiology; D006801:Humans; D007564:Japan / Q000453:epidemiology; D016133:Polymerase Chain Reaction; D017422:Sequence Analysis, DNA; D022622:Shiga Toxin 1
chemical_list: D001427:Bacterial Toxins; D022622:Shiga Toxin 1