title: Interactions of drebrin and gephyrin with profilin.
abstract: Profilin is an actin monomer-binding protein which stimulates actin polymerization. Recent studies have revealed that profilin interacts with VASP, Mena, Bnilp, Bnrlp, and mDia, all of which have the proline-rich domain. Here, we isolated three profilin-binding proteins from rat brain cytosol by glutathione S-transferase-profilin affinity column chromatography and identified them as Mena, drebrin, and gephyrin. These proteins had a proline-rich domain and directly interacted with profilin.
authors.affiliation: Department of Molecular Biology and Biochemistry, Osaka University Medical School, Suita, Japan.
authors.forename: A
authors.initials: A
authors.lastname: Mammoto
authors.forename: T
authors.initials: T
authors.lastname: Sasaki
authors.forename: T
authors.initials: T
authors.lastname: Asakura
authors.forename: I
authors.initials: I
authors.lastname: Hotta
authors.forename: H
authors.initials: H
authors.lastname: Imamura
authors.forename: K
authors.initials: K
authors.lastname: Takahashi
authors.forename: Y
authors.initials: Y
authors.lastname: Matsuura
authors.forename: T
authors.initials: T
authors.lastname: Shirao
authors.forename: Y
authors.initials: Y
authors.lastname: Takai
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001665:Binding Sites / Q000235:genetics; D001921:Brain / Q000378:metabolism; D002352:Carrier Proteins / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D003285:Contractile Proteins; D003600:Cytosol / Q000378:metabolism; D006801:Humans; D066298:In Vitro Techniques; D008565:Membrane Proteins / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D008840:Microfilament Proteins / Q000378:metabolism*; D008969:Molecular Sequence Data; D009479:Neuropeptides / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D010449:Peptide Mapping; D051304:Profilins; D011392:Proline / Q000737:chemistry; D011485:Protein Binding; D051381:Rats; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism
chemical_list: D002352:Carrier Proteins; D003285:Contractile Proteins; D008565:Membrane Proteins; D008840:Microfilament Proteins; D009479:Neuropeptides; C495738:PFN1 protein, human; C495740:Pfn1 protein, rat; D051304:Profilins; D011993:Recombinant Fusion Proteins; C053386:drebrins; C073441:gephyrin; D011392:Proline