title: Lectin histochemistry of rainbow trout (Oncorhynchus mykiss) gill and skin.
abstract: In order to characterize the glycoconjugate residues in skin and gills of the adult rainbow trout, the binding pattern of five biotinylated lectins with different carbohydrate specificities was examined. In the skin, mucous cells revealed binding sites for PNA and SBA; filament-containing cells were additionally labelled with Con A. However, the basal cell layer showed no reaction. In the gill, subpopulations of mucous cells reacted with Con A, PNA, SBA and UEA-I. This broader spectrum of glycoconjugates in gill mucous cells compared with the epidermal mucous cells could point to the additional function of gill mucus in ion and osmoregulation. Lectin binding sites were less common in the respiratory epithelial cells of the secondary lamellae than in those of the primary lamellae. Chloride cells revealed mannose, galactose and fucose residues. Immature chloride cells, as indicated by a comparison with Na+/K+ ATPase immunolabelling, reacted with Con A; subpopulations of them reacted with PNA, SBA and UEA-I. The results form the basis for further investigations in which these cell populations can be analysed under different environmental conditions.
authors.affiliation: Interdisciplinary Centre for General Ecology, University of Berne, Switzerland.
authors.forename: P
authors.initials: P
authors.lastname: Burkhardt-Holm
mesh_terms: D000818:Animals; D004847:Epithelial Cells / Q000737:chemistry / Q000166:cytology; D005880:Gills / Q000737:chemistry* / Q000166:cytology / Q000378:metabolism; D006001:Glycoconjugates / Q000032:analysis* / Q000378:metabolism; D007150:Immunohistochemistry; D037102:Lectins / Q000378:metabolism*; D008297:Male; D009093:Mucus / Q000502:physiology; D017686:Oncorhynchus mykiss / Q000378:metabolism*; D011485:Protein Binding; D012680:Sensitivity and Specificity; D012867:Skin / Q000737:chemistry* / Q000166:cytology
chemical_list: D006001:Glycoconjugates; D037102:Lectins