Retinoic acid suppresses insulin-induced cell growth and cyclin D1 gene expression in human breast cancer cells.
We examined the effects of all-trans retinoic acid (RA) on the insulin-induced cell growth, cell cycle progression and cyclin D1 gene expression in breast cancer cells. RA exerted a dose-dependent growth inhibition on insulin-induced proliferation in T47D and MCF-7 hormone-dependent cell lines, whereas MDA-MB231 hormone-independent cells were not affected. The RA antagonism of insulin growth effect was associated with an inhibition of cell cycle progression and a suppression of insulin-induced cyclin D1 mRNA. The effect of RA on cyclin D1 mRNA was dose-dependent and was observed within 5 h of treatment when insulin response was maximal.
Bardon
S
S
Institut National de la Recherche Agronomique, Laboratoire de Nutrition et Securite Alimentaire, Jouy-en-Josas, 78352, France.
Razanamahefa
L
L
D000970:Antineoplastic Agents / Q000494:pharmacology*; D001943:Breast Neoplasms / Q000188:drug therapy / Q000473:pathology; D002453:Cell Cycle / Q000502:physiology; D002455:Cell Division / Q000187:drug effects* / Q000502:physiology; D002460:Cell Line; D004305:Dose-Response Relationship, Drug; D005260:Female; D015870:Gene Expression / Q000187:drug effects; D019940:Genes, bcl-1 / Q000187:drug effects*; D006801:Humans; D066298:In Vitro Techniques; D007328:Insulin / Q000502:physiology*; D007329:Insulin Antagonists; D012333:RNA, Messenger / Q000187:drug effects / Q000235:genetics; D014212:Tretinoin / Q000494:pharmacology*
D000970:Antineoplastic Agents; D007328:Insulin; D007329:Insulin Antagonists; D012333:RNA, Messenger; D014212:Tretinoin