Growth phase dependent stop codon readthrough and shift of translation reading frame in Escherichia coli.
Nonsense codon readthrough and changed translational reading frame were measured in different growth phases in E. coli. The strains used carry plasmid constructs with a translation assay reporter gene. This reporter gene contains an internal stop codon or a run of U-residues. Termination or frameshifting give rise to stable proteins that can be physically quantified on gels along with the complete protein products. Readthrough of the stop codon UGA by a nearcognate tRNA is several fold higher in active growth than in late exponential phase. In early exponential phase, about 7% of -1 frameshift at a U9 slippery sequence is detectable; upon entry to stationary phase this frameshifting increases to about 40% followed by a decrease in stationary phase. A similar increase is observed in the case of +1 reading frameshift at the U9 sequence, which increases from 13% in early exponential growth phase up to 38% at the beginning of stationary phase followed by a decrease. Thus, the levels of both stop codon readthrough and frameshifting are growth phase dependent, though not in an identical fashion.
Wenthzel
A M
AM
Department of Microbiology, Stockholm University, Sweden.
Stancek
M
M
Isaksson
L A
LA
D001483:Base Sequence; D018388:Codon, Terminator; D004269:DNA, Bacterial; D004926:Escherichia coli / Q000235:genetics* / Q000254:growth & development; D018965:Frameshifting, Ribosomal; D008969:Molecular Sequence Data; D016364:Reading Frames
D018388:Codon, Terminator; D004269:DNA, Bacterial