Expression of the mouse metallothionein-I gene conferring cadmium resistance in a transgenic cyanobacterium.
This paper reports the construction of a transgenic strain of cyanobacterium aimed at removing heavy metal pollution in waters. The mouse metallothionein-I (mMT-I) gene was inserted in the vector pRL-439 downstream of the strong psbA promoter. The resulting plasmid pRL-MT was ligated at the EcoRI site of the shuttle vector pKT-210 to generate the shuttle expression vector pKT-MT. This recombinant plasmid was introduced into Anabaena sp. PCC 7120 by triparental conjugative transfer. After selection on streptomycin, a stable transgenic Anabaena strain was obtained. The presence of the mMT-I gene was confirmed by DNA/DNA hybridization and its expression was demonstrated by immunodetection with specific antibodies. A metal tolerance experiment showed that this transgenic Anabaena strain had acquired higher metal resistance.
Ren
L
L
Ocean University of Qingdao, Shandong, P.R. China.
Shi
D
D
Dai
J
J
Ru
B
B
D017033:Anabaena / Q000187:drug effects / Q000235:genetics*; D000818:Animals; D002104:Cadmium / Q000494:pharmacology*; D003001:Cloning, Molecular; D004352:Drug Resistance, Microbial; D015964:Gene Expression Regulation, Bacterial; D015971:Gene Expression Regulation, Enzymologic; D005822:Genetic Vectors; D008668:Metallothionein / Q000235:genetics*; D051379:Mice; D010957:Plasmids; D019076:Transgenes
D002104:Cadmium; D008668:Metallothionein