title: Bovine type I interferon receptor protein BoIFNAR-1 has high-affinity and broad specificity for human type I interferons.
abstract: The type I interferon receptor (IFNAR) is composed of two transmembrane polypeptides, IFNAR-1 and IFNAR-2. Human IFNAR-1 has low intrinsic affinity for IFNs, but enhances the affinity for IFNs of the complex over that of HuIFNAR-2 alone, and modulates the ligand specificity. Bovine cells respond to human alpha interferons. The bovine homologue of HuIFNAR-1, BoIFNAR-1, when expressed in heterologous cells, confers high-affinity binding and broad specificity for human type I IFNs. A soluble fusion protein of the ectodomain of BoIFNAR-1 and an immunoglobulin Fc domain was produced. In contrast to HuIFNAR-1, this protein competes strongly with human cells for IFN binding, and directly binds a wide spectrum of human type I IFNs, including diverse IFN-alphas, IFN-beta and IFN-omega, with moderate to high affinity. This accounts for much of the specificity for human IFNs possessed by bovine cells, with several exceptions. The BoIFNAR-1 ectodomain, in contrast to HuIFNAR-1, may be useful for studies of binary and ternary complexes with IFNs and IFNAR-2, and for purification, assay and biological neutralization protocols.
authors.affiliation: Department of Molecular Genetics and Microbiology, Robert Wood Johnson Medical School-UMDNJ, Piscataway, NJ 08854, USA. langer@umdnj.edu
authors.forename: J A
authors.initials: JA
authors.lastname: Langer
authors.forename: J
authors.initials: J
authors.lastname: Yang
authors.forename: P
authors.initials: P
authors.lastname: Carmillo
authors.forename: L E
authors.initials: LE
authors.lastname: Ling
mesh_terms: D000818:Animals; D019556:COS Cells; D002417:Cattle; D002460:Cell Line; D006801:Humans; D007370:Interferon Type I / Q000378:metabolism*; D016898:Interferon-alpha / Q000378:metabolism*; D016899:Interferon-beta / Q000378:metabolism*; D008565:Membrane Proteins; D053633:Receptor, Interferon alpha-beta; D017471:Receptors, Interferon / Q000378:metabolism*; D011993:Recombinant Fusion Proteins / Q000378:metabolism
chemical_list: C507964:IFNAR1 protein, human; D007370:Interferon Type I; D016898:Interferon-alpha; D008565:Membrane Proteins; D017471:Receptors, Interferon; D011993:Recombinant Fusion Proteins; C063743:interferon omega 1; D053633:Receptor, Interferon alpha-beta; D016899:Interferon-beta