title: Lack of effect of nicotine or ethanol on the activity of 11beta-hydroxysteroid dehydrogenase type 2.
abstract: Low birth weight in combination with a large placenta predicts human hypertension. The pathophysiological link remains unclear, but glucocorticoid excess impairs fetal growth and leads to offspring hypertension. A key controller of fetal glucocorticoid exposure and local tissue availability is 11beta-hydroxysteroid dehydrogenase type 2 (11beta-HSD2). The activity of placental 11beta-HSD2 correlates with fetal growth in animals and humans. Ethanol abuse and smoking are known to retard fetal growth which may relate to altered glucocorticoid action or dynamics. This study has examined whether nicotine or ethanol modulate glucocorticoid action in the placenta or fetus by inhibiting 11beta-HSD2, using clonal cell cultures, freshly isolated dually perfused intact human placentas and placentas from in vivo treated rats. No significant effect on the activity of 11beta-HSD2 by pathophysiologically relevant nicotine or ethanol concentrations was observed. The mechanism of action of nicotine and ethanol relevant to reduced fetal growth requires further study.
authors.affiliation: University Department of Medicine, Western General Hospital, Edinburgh, Scotland, UK. R.Benediktsson@ed.ac.uk
authors.forename: R
authors.initials: R
authors.lastname: Benediktsson
authors.forename: E M
authors.initials: EM
authors.lastname: Magnusdottir
authors.forename: J R
authors.initials: JR
authors.lastname: Seckl
mesh_terms: D042842:11-beta-Hydroxysteroid Dehydrogenases; D000818:Animals; D000431:Ethanol / Q000494:pharmacology*; D005260:Female; D006801:Humans; D006913:Hydroxysteroid Dehydrogenases / Q000378:metabolism*; D018374:LLC-PK1 Cells; D009538:Nicotine / Q000494:pharmacology*; D010920:Placenta / Q000201:enzymology / Q000378:metabolism; D011247:Pregnancy; D051381:Rats; D017208:Rats, Wistar; D013552:Swine
chemical_list: D000431:Ethanol; D009538:Nicotine; D006913:Hydroxysteroid Dehydrogenases; D042842:11-beta-Hydroxysteroid Dehydrogenases