A two-dimensional protein map of human amniotic fluid at 17 weeks' gestation.
Using updated technical procedures (immobilized pH gradients for isoelectric focusing followed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis: IPG/SDS-PAGE) we provide a two-dimensional (2-D) map of amniotic fluid (AF) proteins. This map comprises over 800 silver-stained spots. Over 150 spots have been identified by matching on the net with human plasma and cerebrospinal fluid maps available from SWISS 2DPAGE database; several additional spots were assigned by immunoblotting and/or microanalytical techniques. This report details our investigation on AF proteins focusing on the 17th week of gestation, when AF is most commonly used for clinical evaluation of fetal disorders. As a whole, the map displays a number of potential markers for fetal development and for gestation abnormalities. The 2-D electrophoretic technique allows the monitoring of all these proteins at the same time along with additional spots that may prove of diagnostic significance.
Liberatori
S
S
Institute of Preventive Pediatrics and Neonatology, Department of Molecular Biology, University of Siena, Italy.
Bini
L
L
De Felice
C
C
Magi
B
B
Marzocchi
B
B
Raggiaschi
R
R
Frutiger
S
S
Sanchez
J C
JC
Wilkins
M R
MR
Hughes
G
G
Hochstrasser
D F
DF
Bracci
R
R
Pallini
V
V
D000328:Adult; D000653:Amniotic Fluid / Q000737:chemistry*; D015180:Electrophoresis, Gel, Two-Dimensional; D005260:Female; D005865:Gestational Age; D006801:Humans; D007525:Isoelectric Focusing; D010449:Peptide Mapping / Q000379:methods*; D011247:Pregnancy