Molecular cloning and expression analysis of the cDNA encoding rat tissue inhibitor of metalloproteinase-4.
We have cloned and sequenced the cDNA encoding the rat tissue inhibitor of metalloproteinase 4 (TIMP-4). As a first step towards exploring the role of TIMP-4 in both the physiological and the pathological remodeling of extracellular matrix, we have extensively analyzed the mRNA expression of TIMP-4 in various rat tissues. The results reveal that the expression pattern of rat TIMP-4 is distinct from that of its human and mouse counterparts (Greene et al., 1996; Leco et al., 1997). In this report, we show that rat TIMP-4 is expressed much more extensively than previously reported, which suggests that it may play a significant role in modulating proteolysis of extracellular matrix in a wide range of tissue settings.
Wu
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Laboratory for Surgical Research, Children's Hospital, Boston, Massachusetts, USA.
Moses
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MA
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000378:metabolism; D004791:Enzyme Inhibitors / Q000378:metabolism; D005109:Extracellular Matrix / Q000378:metabolism; D006801:Humans; D008666:Metalloendopeptidases / Q000037:antagonists & inhibitors; D051379:Mice; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D016415:Sequence Alignment; D019714:Tissue Inhibitor of Metalloproteinases / Q000235:genetics* / Q000378:metabolism*; D000097792:Tissue Inhibitor of Metalloproteinase-4
D018076:DNA, Complementary; D004791:Enzyme Inhibitors; D008666:Metalloendopeptidases; D012333:RNA, Messenger; D019714:Tissue Inhibitor of Metalloproteinases; D000097792:Tissue Inhibitor of Metalloproteinase-4