Parathyroid hormone-related protein (1-37) induces cAMP response in human osteoblast-like cells.
During recent years parathyroid hormone-related protein (PTHrP) research has been focused on the physiological functions of different fragments of the PTHrP molecule. Here we demonstrate that PTHrP (1-37) induced cyclic adenosine monophosphate (cAMP) response in primary human osteoblast-like cells, which were well characterized by the presence of alkaline phosphatase activity and osteocalcin production after stimulation with 1,25-dihydroxyvitamin D3. However, there was no cAMP response to PTHrP (58-77). Furthermore, the response to PTHrP (1-37) was dose dependent, with a significant increase at 1 nM. The presence of PTHrP (1-37)-induced cAMP response in human osteoblast-like cells implies that aminoterminal PTHrP fragments may exert important functions in the bone.
Tsai
J A
JA
Department of Molecular Medicine, Karolinska Hospital and Institute, Stockholm, Sweden.
Bucht
E
E
Stark
A
A
Sjöstedt
U
U
Törring
O
O
D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000469:Alkaline Phosphatase / Q000378:metabolism; D000818:Animals; D016466:CHO Cells / Q000187:drug effects / Q000378:metabolism; D002117:Calcitriol / Q000494:pharmacology; D006224:Cricetinae; D000242:Cyclic AMP / Q000378:metabolism*; D004305:Dose-Response Relationship, Drug; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D010006:Osteoblasts / Q000187:drug effects* / Q000378:metabolism; D015675:Osteocalcin / Q000378:metabolism; D044162:Parathyroid Hormone-Related Protein; D011506:Proteins / Q000494:pharmacology*; D051381:Rats; D019379:Teriparatide / Q000494:pharmacology*; D014407:Tumor Cells, Cultured
C470121:PTHLH protein, human; D044162:Parathyroid Hormone-Related Protein; D011506:Proteins; D015675:Osteocalcin; D019379:Teriparatide; D000242:Cyclic AMP; D000469:Alkaline Phosphatase; D002117:Calcitriol