Changes in protein kinase C and adenylate cyclase in the temporal lobe from subjects with schizophrenia.
Changes in G-protein linked neurotransmitter receptors have been reported in a number of regions of the brain of schizophrenic subjects. These changes, if functional, could cause a change in proteins such as protein kinase C (PKC) and adenylate cyclase (AC) which are important components of the G-protein linked second messenger cascades. We therefore used autoradiography to measure the distribution and density of [3H]phorbol ester binding to PKC and [3H]forskolin binding to AC in tissue obtained at autopsy from schizophrenic and non-schizophrenic subjects (Controls). There were significant decreases in the density of PKC in the parahippocampal gyrus (687 +/- 60 vs. 885 +/- 51 fmol/mg TE; mean +/- SEM; p < 0.01) and in AC in the dentate gyrus (75 +/- 4.9 vs. 92 +/- 6.5, p < 0.05) from the schizophrenic subjects. These data could indicate that changes in neurotransmitter receptors in the hippocampus from subjects with schizophrenia could have resulted in a change in their associated second messenger systems.
Dean
B
B
Mental Health Research Institute of Victoria, Parkville, Australia.
Opeskin
K
K
Pavey
G
G
Hill
C
C
Keks
N
N
D000262:Adenylyl Cyclases / Q000378:metabolism*; D000328:Adult; D000368:Aged; D005576:Colforsin / Q000493:pharmacokinetics; D005260:Female; D005625:Frontal Lobe / Q000201:enzymology / Q000473:pathology; D006624:Hippocampus / Q000201:enzymology / Q000473:pathology; D006801:Humans; D008297:Male; D008875:Middle Aged; D015240:Phorbol 12,13-Dibutyrate / Q000493:pharmacokinetics; D011493:Protein Kinase C / Q000378:metabolism*; D011569:Psychiatric Status Rating Scales; D012559:Schizophrenia / Q000201:enzymology* / Q000473:pathology; D013702:Temporal Lobe / Q000201:enzymology* / Q000473:pathology
D005576:Colforsin; D015240:Phorbol 12,13-Dibutyrate; D011493:Protein Kinase C; D000262:Adenylyl Cyclases