title: Confocal microscopic analysis of the chinchilla organ of Corti following exposure to high-level impact noise.
abstract: To better understand the nature of mechanical changes following exposure to high-level impact noise, normal cochleas and cochleas from chinchillas exposed to either 125 or 131 dB SPL noise were stained with phalloidin for F-actin and examined using confocal microscopy. As seen in previous experiments, 125 dB exposures produced much more variable results than 131 dB exposures. Some cochleas were relatively unscathed by the exposure, whereas others showed damage to outer hair cells (OHCs) immediately after the exposure that included gross distortions of cell bodies and reduced F-actin in cuticular plates. Twenty-four hours later, there was also disorientation of actin filaments in supporting cells. After 30 days, Deiters cells were disarrayed and cups were separated from OHC neural poles. Exposure to noise at a level of 131 dB SPL produced less variable results than 125 dB exposure, and damage was generally more widespread and severe.
authors.affiliation: Center for Hearing and Deafness, University at Buffalo, NY 14214, USA.
authors.forename: V P
authors.initials: VP
authors.lastname: Spongr
authors.forename: D
authors.initials: D
authors.lastname: Henderson
authors.forename: S L
authors.initials: SL
authors.lastname: McFadden
mesh_terms: D000818:Animals; D002682:Chinchilla; D018613:Microscopy, Confocal / Q000379:methods; D009622:Noise / Q000009:adverse effects*; D009925:Organ of Corti / Q000473:pathology*