Suppression of v-src transformation by the drs gene.
Previously, we isolated a novel gene, drs, which was downregulated by retroviral oncogenes such as v-src and v-K-ras, from a cDNA library of primary rat embryo fibroblasts. Experiments using a temperature-sensitive mutant of the v-src gene indicated that downregulation of drs mRNA was dependent on functional expression of v-Src. In addition, expression of drs mRNA was also reduced by serum stimulation of G0-arrested normal rat fibroblast cells. To clarify the function of the drs gene in cell transformation and proliferation, we introduced drs linked to a potent promoter into a normal rat cell line, F2408, and examined the effect of ectopic expression of exogenous drs on the transformation by the v-src gene and growth properties. Cells expressing exogenous drs gene showed significantly decreased efficiency of transformation by v-src irrespective of functional expression of v-Src kinase, while the growth rate and G1/S progression of the cells were not suppressed by expression of exogenous drs gene, indicating that drs has the ability to suppress v-src transformation without disturbing cell proliferation.
Inoue
H
H
Department of Tumor Virology, Research Institute for Microbial Diseases, Osaka University, Japan. hiroinou@biken.osaka-u.ac.jp
Pan
J
J
Hakura
A
A
D000818:Animals; D001358:Avian Sarcoma Viruses / Q000235:genetics; D002453:Cell Cycle; D002455:Cell Division; D002460:Cell Line; D002472:Cell Transformation, Viral; D008565:Membrane Proteins / Q000096:biosynthesis / Q000235:genetics* / Q000502:physiology; D015688:Oncogene Protein pp60(v-src) / Q000235:genetics / Q000378:metabolism*; D012333:RNA, Messenger; D051381:Rats
D008565:Membrane Proteins; D012333:RNA, Messenger; C099179:Srpx protein, rat; D015688:Oncogene Protein pp60(v-src)