The characterization of chicken antibodies raised against Fusarium spp. by enzyme-linked immunosorbent assay and immunoblotting.
Immunoassays for the detection of three Fusarium species have been developed. Chickens were immunized with the soluble antigens from within the mycelia (mycelia-soluble antigens) and the exoantigens of Fusarium sporotrichioides, Fusarium poae and Fusarium graminearum and the antigens were characterized using enzyme-linked immunosorbent assays, immunoblotting and sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE). The cross-reactivities of antisera with eleven species of Fusarium, eleven species of fungi from other genera and the buffer-extracts of grain were determined. Antisera to the exoantigens and mycelia-soluble antigens did not cross-react with buffer-extracts of grain. The antisera against the mycelia-soluble antigens tended to cross-react with the antigens from other genera whereas the antisera against the exoantigens were genus specific. The antiserum raised against exoantigens from F. poae was species specific. The molecular weights of the immunodominant antigens from the Fusaria were above 28 kilodaltons. Antigens from Fusaria showed specific bands in SDS-PAGE gel that can be used to produce more specific antibodies. The results suggest that exoantigen immunoassay can be developed to identify Fusarium genus, whereas mycelia-soluble antigens are not suitable for immunoidentification among genus.
Gan
Z
Z
Department of Animal Science, University of Manitoba, Winnipeg, Canada.
Marquardt
R R
RR
Abramson
D
D
Clear
R M
RM
D000818:Animals; D000908:Antibodies, Fungal / Q000276:immunology*; D000946:Antigens, Fungal / Q000032:analysis / Q000276:immunology; D002645:Chickens; D003429:Cross Reactions; D004797:Enzyme-Linked Immunosorbent Assay; D005670:Fusarium / Q000276:immunology*; D007106:Immune Sera / Q000276:immunology; D015151:Immunoblotting; D016622:Silver Staining
D000908:Antibodies, Fungal; D000946:Antigens, Fungal; D007106:Immune Sera