title: Intermittent cyclic loading of cartilage explants modulates fibronectin metabolism.
abstract: OBJECTIVE
  The aim of this study was to evaluate systematically the effect of tissue load, its amplitude, time of intermittence and duration of loading on the biosynthesis and release of fibronectin by intermittently loaded mature bovine articular cartilage explants.
  
  
  METHODS
  Cyclic compressive pressure was introduced using a sinusoidal waveform of 0.5 Hz-frequency with a peak stress of 0.1, 0.5 or 1.0 MPa for a period of 10 s followed by an unloaded period lasting 10, 100 or 1000 s. Fibronectin and total proteins were radiolabeled with 10 microCi/ml [3H]-phenylalanine during the final 18 h of the 1, 3 or 6 day experiments. The content of endogenous fibronectin was determined using enzyme-linked immunosorbant assay (ELISA), whereas the viability of explants was measured using sections of cartilage explants stained with fluorescein diacetate and propidium iodide. The deformation of loaded explants was determined using a load-displacement transducer system.
  
  
  RESULTS
  The mechanical factor time of intermittence significantly altered the synthesis and release of fibronectin by cartilage explants, whereas the tested range of load magnitudes, as well as the duration of loading, seemed to be of subordinate importance. Loading affected the viability of the superficial zone in the cartilage, whereas the chondrocytes of the intermediate and deep zone remained viable. The compression of loaded explants was dependent on the magnitude of stress, as well as on the duration of unloading between each loading cycle. Synthesis of fibronectin, the retention of newly synthesized fibronectin within the extracellular matrix, and the portion of newly synthesized proteins that were fibronectin was significantly increased in cartilage explants which were cyclically compressed with 0.5 MPa for 10 s followed by a period of unloading lasting 100 s.
  
  
  CONCLUSIONS
  Previous studies reporting that cartilage explants of human and animal osteoarthritic joints synthesize and retain elevated amounts of fibronectin imply that in our experiments mechanical stimuli can induce a fibronectin metabolism in vitro which mimics some of the osteoarthritic characteristics.
authors.affiliation: Department of Pharmacology and Toxicology, Rheinische Friedrich-Wilhelms-Universität Bonn, Germany.
authors.forename: J
authors.initials: J
authors.lastname: Steinmeyer
authors.forename: B
authors.initials: B
authors.lastname: Ackermann
authors.forename: R X
authors.initials: RX
authors.lastname: Raiss
mesh_terms: D000818:Animals; D002358:Cartilage, Articular / Q000378:metabolism* / Q000473:pathology; D002417:Cattle; D002470:Cell Survival; D019902:Chondrocytes / Q000473:pathology; D046508:Culture Techniques; D005353:Fibronectins / Q000378:metabolism*; D008297:Male; D010003:Osteoarthritis / Q000209:etiology / Q000378:metabolism*; D011312:Pressure; D011506:Proteins / Q000378:metabolism; D013314:Stress, Mechanical; D013997:Time Factors
chemical_list: D005353:Fibronectins; D011506:Proteins