title: Direct detection of viable bacteria, molds, and yeasts by reverse transcriptase PCR in contaminated milk samples after heat treatment.
abstract: A fast, sensitive, and target contaminant-modulable method was developed to detect viable bacteria, molds, and yeasts after heat treatment. By reverse transcriptase PCR with elongation factor gene (EF-Tu or EF-1 alpha)-specific primers, the detection level was 10 cells ml of milk-1. The simplicity and rapidity (4 h) of the procedure suggests that this method may be easily transposable to other foods and other contaminants.
authors.affiliation: Tepral, Beverage Division Research Center of Danone Group, Strasbourg, France.
authors.forename: M
authors.initials: M
authors.lastname: Vaitilingom
authors.forename: F
authors.initials: F
authors.lastname: Gendre
authors.forename: P
authors.initials: P
authors.lastname: Brignon
mesh_terms: D000818:Animals; D001419:Bacteria / Q000302:isolation & purification*; D005658:Fungi / Q000302:isolation & purification*; D006358:Hot Temperature; D008892:Milk / Q000382:microbiology*; D010444:Peptide Elongation Factor Tu / Q000235:genetics; D016133:Polymerase Chain Reaction; D015003:Yeasts / Q000302:isolation & purification*
chemical_list: D010444:Peptide Elongation Factor Tu