Antianaphylactic properties of eugenol.
The effects of eugenol, a major component of clove, on anaphylaxis were evaluated in rats. Eugenol inhibited compound 48/80-induced systemic anaphylaxis 100% with a dose of 10 micrograms g-1 body weight (BW). While serum levels of histamine were markedly elevated after compound 48/80 injection in all groups of rats, rats injected with eugenol showed a significant reduction in serum histamine levels. Eugenol also inhibited passive cutaneous anaphylaxis activated by anti-dinitrophenyl (DNP) IgE. Eugenol dose-dependently inhibited histamine release from the rat peritoneal mast cells (RPMC) activated by compound 48/80 or anti-DNP IgE. The morphological examination clearly showed that eugenol prevented the anaphylactic degranulation of RPMC. Moreover, Eugenol (10 micrograms ml-1) had a significant inhibitory effect on anti-DNP IgE-induced tumor necrosis factor-alpha production. These results suggest that eugenol has antianaphylactic properties by preventing mast cell degranulation.
Kim
H M
HM
Department of Oriental Pharmacy, Wonkwang University, Iksan, Chonbuk, South Korea.
Lee
E H
EH
Kim
C Y
CY
Chung
J G
JG
Kim
S H
SH
Lim
J P
JP
Shin
T Y
TY
D000707:Anaphylaxis / Q000139:chemically induced / Q000188:drug therapy*; D000818:Animals; D015550:Cell Degranulation / Q000187:drug effects; D004140:Dinitrophenols / Q000276:immunology; D005054:Eugenol / Q000494:pharmacology* / Q000627:therapeutic use*; D005070:Evans Blue; D006632:Histamine / Q000097:blood; D007073:Immunoglobulin E / Q000494:pharmacology; D066298:In Vitro Techniques; D017737:Macrophages, Peritoneal / Q000166:cytology / Q000187:drug effects / Q000276:immunology; D008407:Mast Cells / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D010323:Passive Cutaneous Anaphylaxis / Q000187:drug effects; D051381:Rats; D017208:Rats, Wistar; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism; D003189:p-Methoxy-N-methylphenethylamine
D004140:Dinitrophenols; D014409:Tumor Necrosis Factor-alpha; D007073:Immunoglobulin E; D005054:Eugenol; D003189:p-Methoxy-N-methylphenethylamine; D005070:Evans Blue; D006632:Histamine