Blockade of CD40-CD154 interferes with human T cell engraftment in scid mice.
Antibodies to the ligand for CD40 (CD154) have been shown to exert profound effects on the development of cell-mediated immune responses in mice. The present study shows that an antibody to human CD154 (hCD40L) inhibits in vivo Tetanus toxoid (TT) specific secondary antibody responses in hu-PBL-scid mice, as well as the expansion of xenoreactive human T cells in the scid mice. A possible cause for the reduced expansion of xenoreactive, human T cells, was the decreased expression of murine B7.1 and B7.2 caused by the administration of anti-hCD40L. Therefore, it may be that defective maturation of murine antigen-presenting cells impeded the priming and expansion of human xenoreactive T cells.
Foy
T M
TM
Department of Microbiology, Dartmouth Medical School, Lebanon, NH 03756, USA.
McIlraith
M
M
Masters
S R
SR
Dunn
J J
JJ
Rossini
A A
AA
Shultz
L D
LD
Hesselton
R A
RA
Wagar
E J
EJ
Lipsky
P E
PE
Noelle
R J
RJ
Greiner
D L
DL
D000328:Adult; D000818:Animals; D000911:Antibodies, Monoclonal; D015703:Antigens, CD / Q000378:metabolism; D001402:B-Lymphocytes / Q000166:cytology / Q000276:immunology; D018122:B7-1 Antigen / Q000378:metabolism; D051940:B7-2 Antigen; D019013:CD40 Antigens / Q000378:metabolism*; D023201:CD40 Ligand; D002454:Cell Differentiation; D017690:Cell Transplantation; D006085:Graft Survival / Q000276:immunology; D006801:Humans; D008024:Ligands; D008213:Lymphocyte Activation; D008562:Membrane Glycoproteins / Q000037:antagonists & inhibitors* / Q000378:metabolism; D051379:Mice; D016513:Mice, SCID; D013601:T-Lymphocytes / Q000276:immunology* / Q000637:transplantation*; D013745:Tetanus Toxoid / Q000276:immunology; D014183:Transplantation, Heterologous
D000911:Antibodies, Monoclonal; D015703:Antigens, CD; D018122:B7-1 Antigen; D051940:B7-2 Antigen; D019013:CD40 Antigens; C497964:CD86 protein, human; C497965:Cd86 protein, mouse; D008024:Ligands; D008562:Membrane Glycoproteins; D013745:Tetanus Toxoid; D023201:CD40 Ligand