Antisense oligonucleotides and myotonin gene expression in C2 mouse cells.
By describing the behavior of myotonin mRNA levels, from the quiescent to the differentiated state in C2 mouse myoblasts, we produced evidence bearing on the role of myotonin gene product in the control of cell growth and differentiation. To study the role of myotonin in myotonic dystrophy (DM) pathogenesis, we developed a suitable cellular model where myotonin gene expression was modulated by phosphorothioate antisense oligonucleotides in C2 cultured cells. Furthermore, an isoform of the gene product, similar to that described in humans and not yet described in the mouse, was found.
Melone
M A
MA
2nd Division of Neurology, 2nd University of Naples, School of Medicine, Italy.
Galderisi
U
U
Iacomino
G
G
Cipollaro
M
M
Di Bernardo
G
G
Cotrufo
R
R
Peluso
G
G
Cascino
A
A
D000818:Animals; D002454:Cell Differentiation; D002460:Cell Line; D004339:Drug Compounding; D015870:Gene Expression; D008081:Liposomes; D051379:Mice; D008809:Mice, Inbred C3H; D018482:Muscle, Skeletal / Q000166:cytology / Q000378:metabolism*; D065636:Myotonin-Protein Kinase; D016376:Oligonucleotides, Antisense / Q000494:pharmacology*; D011494:Protein Kinases; D017346:Protein Serine-Threonine Kinases / Q000096:biosynthesis* / Q000235:genetics; D012333:RNA, Messenger / Q000032:analysis; D013873:Thionucleotides / Q000494:pharmacology*
C585517:DMPK protein, human; C585516:DMPK protein, mouse; D008081:Liposomes; D016376:Oligonucleotides, Antisense; D012333:RNA, Messenger; D013873:Thionucleotides; D011494:Protein Kinases; D065636:Myotonin-Protein Kinase; D017346:Protein Serine-Threonine Kinases