Three cis-elements required for rice alpha-amylase Amy3D expression during sugar starvation.
Expression of alpha-amylase genes during seedling development plays a key role in production of sugar from the starch stored in the cereal seed. Rice alpha-amylase Amy3D promoter/GUS constructs in transgenic rice cell lines were studied to identify cis elements in the promoter of this metabolite-regulated gene. Three sequences having the greatest effects on Amy3D gene expression included the amylase element (TATCCAT), the CGACG element, and a G box-related element (CTACGTGGCCA). These promoter cis elements are needed for high-level expression of Amy3D under conditions of sugar starvation. The involvement of G box cis-elements in environmental stress responses suggest a link between the nutrient stress and the environmental stress responses of the plant.
Hwang
Y S
YS
Section of Molecular and Cellular Biology, University of California, Davis 95616, USA.
Karrer
E E
EE
Thomas
B R
BR
Chen
L
L
Rodriguez
R L
RL
D001483:Base Sequence; D050260:Carbohydrate Metabolism; D002460:Cell Line; D018507:Gene Expression Regulation, Developmental; D015971:Gene Expression Regulation, Enzymologic; D018506:Gene Expression Regulation, Plant; D017343:Genes, Plant; D017930:Genes, Reporter; D008969:Molecular Sequence Data; D012275:Oryza / Q000201:enzymology* / Q000235:genetics / Q000502:physiology; D030821:Plants, Genetically Modified; D011401:Promoter Regions, Genetic; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis; D012045:Regulatory Sequences, Nucleic Acid; D017384:Sequence Deletion; D016385:TATA Box; D014162:Transfection; D000516:alpha-Amylases / Q000096:biosynthesis*
D011993:Recombinant Fusion Proteins; D000516:alpha-Amylases