title: Cytotoxicity of denture base resins.
abstract: This in vitro study examined the effect of eluate from heat-activated, chemically activated, and microwave-activated denture base resins on cell viability of primary cultures of human gingival fibroblasts. Eluates corresponding to 24, 48, 72, and 96 hours of resin disk immersion were prepared. Fibroblasts were plated at a density of 3 x 10(4) cells in 96-well plates and exposed to a medium containing eluate. After 24 hours, the cytotoxic effect was determined by cellular mitochondrial function. The effect of eluates was compared to control cultures containing culture medium without eluate. Results indicated that at all time periods tested, all three resins leached materials that were cytotoxic to the fibroblasts. Eluate from chemically activated resin disks was more cytotoxic than eluate from heat-activated and microwave-activated disks. In general, cytotoxicity appeared to diminish as disk immersion time was increased. The greatest cytotoxic effect on cell viability was observed with eluates recovered after 24 hours of disk immersion, and the least cytotoxic effect was observed with eluates recovered after 96 hours of immersion.
authors.affiliation: Department of Otolaryngology-Head and Neck Surgery, University of Nebraska Medical Center, Omaha 68583-0750, USA.
authors.forename: P J
authors.initials: PJ
authors.lastname: Sheridan
authors.forename: S
authors.initials: S
authors.lastname: Koka
authors.forename: N O
authors.initials: NO
authors.lastname: Ewoldsen
authors.forename: C A
authors.initials: CA
authors.lastname: Lefebvre
authors.forename: M T
authors.initials: MT
authors.lastname: Lavin
mesh_terms: D000180:Acrylic Resins / Q000737:chemistry / Q000633:toxicity*; D000704:Analysis of Variance; D002478:Cells, Cultured; D003819:Denture Bases; D005347:Fibroblasts / Q000187:drug effects; D005881:Gingiva / Q000166:cytology / Q000187:drug effects; D006358:Hot Temperature; D006801:Humans; D008872:Microwaves; D013997:Time Factors
chemical_list: D000180:Acrylic Resins; C075051:Acron MC; C040291:Lucitone