DNA regions supporting hippocalcin gene expression in cell lines.
The rat hippocalcin gene -3.2 to +0.6 kb region activates reporter gene expression in the NG108-15 and PC12 neuronal cell lines, but not in NIH3T3 or HEK-293 cells. Three fragments (-3.2 to -2.6, -2.6 to -2.3 and -2.3 to -1.8 kb) weakly activate transcription, and "-1.8 to -1.5" kb is a strong activator. Thus cell type-specific expression of the rat hippocalcin gene is regulated by distributed elements in the -3.2 to -1.5 kb region.
Grant
A L
AL
MRC Laboratory of Molecular Biology, MRC Centre, Cambridge, UK.
Wisden
W
W
D016475:3T3 Cells; D000818:Animals; D002135:Calcium-Binding Proteins / Q000096:biosynthesis* / Q000235:genetics*; D002460:Cell Line; D015500:Chloramphenicol O-Acetyltransferase / Q000096:biosynthesis; D004247:DNA / Q000235:genetics*; D004273:DNA, Neoplasm / Q000235:genetics; D005910:Glioma; D051597:Hippocalcin; D006801:Humans; D051379:Mice; D008969:Molecular Sequence Data; D009419:Nerve Tissue Proteins; D009447:Neuroblastoma; D009474:Neurons / Q000378:metabolism; D016716:PC12 Cells; D051381:Rats; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis; D014158:Transcription, Genetic; D014162:Transfection
D002135:Calcium-Binding Proteins; D004273:DNA, Neoplasm; C496691:HPCA protein, human; C496692:Hpca protein, mouse; C496693:Hpca protein, rat; D009419:Nerve Tissue Proteins; D011993:Recombinant Fusion Proteins; D051597:Hippocalcin; D004247:DNA; D015500:Chloramphenicol O-Acetyltransferase