Application of a Fas ligand encoding a recombinant adenovirus vector for prolongation of transgene expression.
An adenovirus vector encoding murine Fas ligand (mFasL) under an inducible control was derived. In vivo ectopic expression of mFasL in murine livers induced an inflammatory cellular infiltration. Furthermore, ectopic expression of mFasL by myocytes did not allow prolonged vector-mediated transgene expression. Thus, ectopic expression of functional mFasL in vector-transduced cells does not appear to confer, by itself, an immunoprivileged site sufficient to mitigate adenovirus vector immunogenicity.
Zhang
H G
HG
Gene Therapy Program, Department of Rheumatology, University of Alabama at Birmingham, 35294, USA.
Bilbao
G
G
Zhou
T
T
Contreras
J L
JL
Gómez-Navarro
J
J
Feng
M
M
Saito
I
I
Mountz
J D
JD
Curiel
D T
DT
D000256:Adenoviridae / Q000235:genetics / Q000276:immunology*; D000818:Animals; D002461:Cell Line, Transformed; D002478:Cells, Cultured; D053222:Fas Ligand Protein; D015870:Gene Expression; D005822:Genetic Vectors / Q000235:genetics / Q000276:immunology*; D006801:Humans; D019426:Integrases / Q000378:metabolism; D007763:Lac Operon; D008024:Ligands; D008264:Macrophages / Q000166:cytology / Q000378:metabolism; D008562:Membrane Glycoproteins / Q000096:biosynthesis / Q000235:genetics* / Q000276:immunology; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D019463:Mice, Inbred MRL lpr; D011995:Recombination, Genetic; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology; D013997:Time Factors; D019076:Transgenes; D014764:Viral Proteins
C505661:FASLG protein, human; D053222:Fas Ligand Protein; C505658:Fasl protein, mouse; D008024:Ligands; D008562:Membrane Glycoproteins; D014764:Viral Proteins; C045073:Cre recombinase; D019426:Integrases