Acquired immunodeficiency syndrome-associated Kaposi's sarcoma and human herpesvirus 8 DNA detection in serial peripheral blood mononuclear cell samples.
Human herpesvirus 8 (HHV8) DNA was amplified from peripheral blood mononuclear cells (PBMCs) using PCR in 120 HIV-seropositive in- and outpatients who were enrolled in a cohort study between January 1994 and June 1995. Risk factors for HIV infection were homosexuality/bisexuality alone in 64 cases (30 with Kaposi's sarcoma (KS) and 34 without KS, 4 of whom had KS lesions that appeared during follow-up in the cohort), heterosexual contact alone in 32 cases (among whom 1 woman with KS who was the spouse of a bisexual with KS), and transfusion of blood or blood products alone in 24 cases. Three blood samples at 3-4-month intervals were scheduled for each patient. Twenty-five HIV1-seronegative patients served as controls. A total of 47.1% of homo- or bisexual males with KS and 26.7% of homo- or bisexual males without KS had positive HHV8 DNA detection as compared with 21.9% of patients contaminated by heterosexual contact, 8.3% of blood product recipients and 0% of controls. HHV8 DNA detection was intermittent in all but 3 patients according to sequential sampling. Multivariate analysis showed that AIDS-KS was associated with sexual transmission, mainly homo- or bisexual practices and with HHV8 infection assessed by PCR in PBMCs.
Dupon
M
M
Département de Maladies infectieuses et de Médecine interne, Hôpital Pellegrin, Bordeaux, France.
Masquelier
B
B
Cazorla
C
C
Chêne
G
G
Dumon
B
B
Ragnaud
J M
JM
de Barbeyrac
B
B
Bébéar
C
C
Lacut
J Y
JY
Fleury
H J
HJ
D000328:Adult; D000704:Analysis of Variance; D015139:Blotting, Southern; D018791:CD4 Lymphocyte Count; D004279:DNA, Viral / Q000097:blood* / Q000302:isolation & purification*; D005260:Female; D006679:HIV Seropositivity / Q000097:blood; D019288:Herpesvirus 8, Human / Q000235:genetics / Q000302:isolation & purification*; D006801:Humans; D007963:Leukocytes, Mononuclear / Q000821:virology*; D008297:Male; D008875:Middle Aged; D009000:Monocytes / Q000502:physiology; D016133:Polymerase Chain Reaction; D012514:Sarcoma, Kaposi / Q000097:blood / Q000175:diagnosis* / Q000821:virology
D004279:DNA, Viral