title: Mouse spermatogenic cell-specific type 1 hexokinase (mHk1-s) transcripts are expressed by alternative splicing from the mHk1 gene and the HK1-S protein is localized mainly in the sperm tail.
abstract: Unique type 1 hexokinase (HK1) mRNAs are present in mouse spermatogenic cells (mHk1-s). They encode a spermatogenic cell-specific sequence region (SSR) but not the porin-binding domain (PBD) necessary for HK1 binding to porin on the outer mitochondrial membrane. This study determined the origin of the multiple Hk1-s transcripts in mouse spermatogenic cells and verified that they are translated in mouse spermatogenic cells. It also showed that a single mHk1 gene encodes the mHk1 transcripts of somatic cells and the mHk1-sa and mHk1-sb transcripts of spermatogenic cells, that alternative exons are used during mHk1 gene expression in mouse spermatogenic cells, and that mHK1-S is translated in mouse spermatogenic cells and is localized mainly with the fibrous sheath in the tail region, not with the mitochondria in the midpiece of mouse sperm.
authors.affiliation: Department of Anatomy and Developmental Biology, Faculty of Medicine, Kyoto University, Japan. mori@med.kyoto-u.ac.jp
authors.forename: C
authors.initials: C
authors.lastname: Mori
authors.forename: N
authors.initials: N
authors.lastname: Nakamura
authors.forename: J E
authors.initials: JE
authors.lastname: Welch
authors.forename: H
authors.initials: H
authors.lastname: Gotoh
authors.forename: E H
authors.initials: EH
authors.lastname: Goulding
authors.forename: M
authors.initials: M
authors.lastname: Fujioka
authors.forename: E M
authors.initials: EM
authors.lastname: Eddy
mesh_terms: D017398:Alternative Splicing / Q000235:genetics*; D000818:Animals; D000906:Antibodies / Q000737:chemistry; D001483:Base Sequence; D005091:Exons; D018507:Gene Expression Regulation, Developmental; D006593:Hexokinase / Q000235:genetics* / Q000276:immunology; D008297:Male; D051379:Mice; D008813:Mice, Inbred ICR; D008969:Molecular Sequence Data; D013082:Sperm Tail / Q000201:enzymology*; D013091:Spermatogenesis / Q000235:genetics*; D013737:Testis / Q000201:enzymology; D014158:Transcription, Genetic
chemical_list: D000906:Antibodies; D006593:Hexokinase