Genomic structure and chromosomal localization of the mouse LIM domain-binding protein 1 gene, Ldb1.
The LIM domain is a structural motif that is well conserved throughout evolution in a variety of factors known to play important roles in development and cell regulation. Ldb genes encode LIM domain-binding (Ldb) factors. Here we report on the structural organization and chromosomal localization of the mouse Ldb1 gene. It contains at least 10 exons and spans approximately 4 kb of genomic DNA. The transcription initiation site is located 462 bp upstream of the translation initiation codon ATG as determined by 5'-RACE. Sequencing analysis of the 5'-flanking region shows TATA and CCAAT motifs as well as potential binding sites for GATA, CF-1, PEA3, HRE, APRRE, RARE, Myc, and c-Jun. Ldb1 maps to the distal region of mouse chromosome 19 that is syntenic with human chromosome 10q.
Yamashita
T
T
Laboratory of Mammalian Genes and Development, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, Maryland 20892-2790, USA.
Agulnick
A D
AD
Copeland
N G
NG
Gilbert
D J
DJ
Jenkins
N A
NA
Westphal
H
H
D000818:Animals; D001483:Base Sequence; D002874:Chromosome Mapping; D004247:DNA; D004268:DNA-Binding Proteins / Q000235:genetics*; D005091:Exons; D007438:Introns; D060588:LIM Domain Proteins; D051379:Mice; D008969:Molecular Sequence Data; D014176:Protein Biosynthesis; D014157:Transcription Factors; D014158:Transcription, Genetic
D004268:DNA-Binding Proteins; C486312:LDB1 protein, human; D060588:LIM Domain Proteins; C102084:Ldb1 protein, mouse; D014157:Transcription Factors; D004247:DNA