title: [Effects of fractionation and dose rate in PDR brachytherapy of B14 cells].
abstract: OBJECTIVE
  Present radiobiological studies for different cell lines in vitro demonstrate the equivalence and efficacy of continuous low-dose-rate brachytherapy (LDR-BT) and pulsed dose rate brachytherapy (PDR-BT) when using small and frequent dose pulses. The aim of this study was to examine monolayer fibroblast cultures in vitro to examine the biological effects of different pulse doses and dose rates under clinically conditions.
  
  
  METHODS
  B14 cells, Hy B14 FAF 28, peritoneal fibroblasts, were cultured in multi-well plates and exposed to a PDR radiation source at a distance of 9 mm. The following PDR-schemes were compared: dose per pulse: 1 Gy, 2.5 Gy and 5 Gy to a total dose of 5 Gy/5 h (overall time), 10 Gy/10 h, 20 Gy/20 h and 30 Gy/30 h. The pulse duration for the examination of dose rate effects was 20 min, 30 min or 52 min corresponding by dye pulse dose rate of 300 cGy/h, 200 cGy/h or 115 cGy/h. Treatment endpoints were cell measured by dye exclusion test and clonogenic cell survival.
  
  
  RESULTS
  Cell survival decreased for pulse doses of 5 Gy compared to 2.5 Gy or 1 Gy per pulse (mean dose rate 200 to 300 cGy/h). No differences were observed with dose rates during irradiation of 300 cGy/h, 200 cGy/h or 115 cGy/h (20 Gy/1 Gy).
  
  
  CONCLUSIONS
  Radiobiological effects of PDR-RT are dependent on the dose per pulse, with differences in biological effects only with a dose per pulse of more than 2.5 Gy, considering the described in-vitro conditions. More examinations with a more pronounced difference in dose rate will be continued for evaluation of dose rate effects.
authors.affiliation: Strahlentherapeutische Klinik, Universität, Erlangen-Nürnberg.
authors.forename: L
authors.initials: L
authors.lastname: Keilholz
authors.forename: M H
authors.initials: MH
authors.lastname: Seegenschmiedt
authors.forename: M
authors.initials: M
authors.lastname: Lotter
authors.forename: R
authors.initials: R
authors.lastname: Schulz-Wendtland
authors.forename: J
authors.initials: J
authors.lastname: von Erffa
authors.forename: S
authors.initials: S
authors.lastname: Pflüger
authors.forename: R
authors.initials: R
authors.lastname: Sauer
mesh_terms: D000818:Animals; D001918:Brachytherapy / Q000379:methods*; D002460:Cell Line; D002470:Cell Survival / Q000528:radiation effects; D006224:Cricetinae; D003412:Cricetulus; D019583:Dose Fractionation, Radiation; D004307:Dose-Response Relationship, Radiation; D005347:Fibroblasts / Q000528:radiation effects; D011879:Radiotherapy Dosage; D013997:Time Factors
vernacular_title: Effekte von Fraktionierung und Dosisleistung bei der PDR-Brachytherapie von B14-Zellen.