Assay for erythrocyte superoxide dismutase activity in patients with lung cancer and effects on pollution and smoke trace elements.
The antioxidative effect of CuZnSOD, which catalyzes the dismutation of superoxide anion (O2-), provides a defense against the oxygen toxicity. The object of the study is to evaluate the erythrocytes superoxide dismutase (SOD) activity in two groups of persons (Group I, healthy blood donors; Group II, lung cancer patients), using the spectrophotometric assay of NADH oxidation and the indirect method. The effect of trace elements, such as Al3-, Cr3+, Fe3+, Hg2+, NI2+, and Pb2+ (producing free radicals oxygen and present in pollution and smoke) is also evaluated. The results show the decrease of SOD activity in lung cancer patients with respect to healthy individuals. Likewise, in those patients the enzymatic activity is bigger in early stage (I,II) with respect to advanced one (III) (p < 0.05). The lesser activity when the samples are incubated with Ni or Pb point out that these metals play a role in neoplasm development. In short, the oxidant-antioxidant balance is altered in lung cancer patients.
Martin-Mateo
M C
MC
Department of Biochemistry, Molecular Biology & Physiology, Faculty of Science, Paseo del Prado de la Magdalena s/n, Valladolid, Spain.
Molpeceres
L M
LM
Ramos
G
G
D000230:Adenocarcinoma / Q000097:blood / Q000201:enzymology / Q000209:etiology; D000368:Aged; D018287:Carcinoma, Large Cell / Q000097:blood / Q000201:enzymology / Q000209:etiology; D002289:Carcinoma, Non-Small-Cell Lung / Q000097:blood / Q000201:enzymology / Q000209:etiology; D018288:Carcinoma, Small Cell / Q000097:blood / Q000201:enzymology / Q000209:etiology; D002294:Carcinoma, Squamous Cell / Q000097:blood / Q000201:enzymology / Q000209:etiology; D004785:Environmental Pollutants / Q000009:adverse effects* / Q000032:analysis; D004912:Erythrocytes / Q000187:drug effects / Q000201:enzymology*; D005260:Female; D005609:Free Radicals; D006801:Humans; D008175:Lung Neoplasms / Q000097:blood / Q000201:enzymology* / Q000209:etiology; D008297:Male; D008875:Middle Aged; D009243:NAD / Q000737:chemistry; D009367:Neoplasm Staging; D010084:Oxidation-Reduction; D013056:Spectrophotometry, Ultraviolet; D013482:Superoxide Dismutase / Q000097:blood*; D014028:Tobacco Smoke Pollution / Q000009:adverse effects* / Q000032:analysis; D014131:Trace Elements / Q000009:adverse effects* / Q000032:analysis
D004785:Environmental Pollutants; D005609:Free Radicals; D014028:Tobacco Smoke Pollution; D014131:Trace Elements; D009243:NAD; D013482:Superoxide Dismutase