Preparation of PCR products for DNA sequencing.
We demonstrate that routine PCR product analytical agarose gels can also serve as preparative gels for quick DNA template purification before sequencing. The band of interest is excised, placed into a Gel Nebulizer inside a Micropure separator and rapidly purified in a single centrifugation step. Gel-purified PCR product, suitable for manual and automated sequencing, is delivered within 10 min.
Leonard
J T
JT
Millipore, Danvers, MA, USA.
Grace
M B
MB
Buzard
G S
GS
Mullen
M J
MJ
Barbagallo
C B
CB
D002498:Centrifugation / Q000379:methods; D004247:DNA / Q000302:isolation & purification*; D003956:Dialysis / Q000379:methods; D004587:Electrophoresis, Agar Gel; D016133:Polymerase Chain Reaction / Q000379:methods*; D017422:Sequence Analysis, DNA / Q000379:methods*
D004247:DNA