Escherichia coli transformant expressing the glucose dehydrogenase gene from Bacillus megaterium as a cofactor regenerator in a chiral alcohol production system.
Escherichia coli JM109 (pGDA2) overexpressing the glucose dehydrogenase (GDH) gene from Bacillus megaterium IWG3 was examined for use as a cofactor regenerator. In the asymmetric reduction of ethyl 4-chloro-3-oxobutanoate by E. coli JM109 (pKAR) which is an aldehyde reductase-overproducing transformant, E. coli JM109 (pGDA2) can act as an NADPH regenerator with NADP+ and glucose, similarly to commercially available GDH.
Kataoka
M
M
Division of Applied Life Sciences, Graduate School of Agriculture, Kyoto University, Japan.
Sri Rohani
L P
LP
Wada
M
M
Kita
K
K
Yanase
H
H
Urabe
I
I
Shimizu
S
S
D000438:Alcohols / Q000378:metabolism*; D001410:Bacillus megaterium / Q000201:enzymology* / Q000235:genetics; D004926:Escherichia coli / Q000378:metabolism*; D015870:Gene Expression; D005948:Glucose Dehydrogenases / Q000096:biosynthesis / Q000235:genetics / Q000378:metabolism*; D009249:NADP / Q000096:biosynthesis*
D000438:Alcohols; D009249:NADP; D005948:Glucose Dehydrogenases