In vitro cytotoxicity of BTEX metabolites in HeLa cells.
Fuel leakage from underground storage tanks is a major source of groundwater contamination. Although the toxicity of regulated compounds such as benzene, toluene, ethylbenzene, and xylene (BTEX) are well recognized, the cytotoxicity of their metabolites has not been studied extensively. In this study, Hela cells, propagated at 37 degrees C in an atmosphere of 5% CO2-95% air, served as a target for evaluation of cytotoxicity of BTEX metabolites 3-methylcatechol, 4-methylcatechol, 4-hydroxybenzoic acid, and 4-hydroxy-3-methoxybenzoic acid. The cells were exposed to different concentrations of the metabolites, which subsequently showed inhibition of cell growth and produced dose-related decreases in cell viability and cell protein content. The BTEX metabolites affected the levels of the polyamines spermidine, spermine, and putrescine, which are known to be important in cell proliferation. The cytotoxic effects for these BTEX metabolites to Hela cells were 3-methylcatechol > 4-methylcatechol > 4-hydroxy-3-methoxybenzoic acid > 4-hydroxybenzoic acid.
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National Risk Management Research Laboratory, Subsurface Protection & Remediation Division, U.S. Environmental Protection Agency, Ada, Oklahoma 74820, USA.
D001555:Benzene Derivatives / Q000378:metabolism / Q000633:toxicity; D002470:Cell Survival / Q000187:drug effects; D006367:HeLa Cells; D006801:Humans; D006838:Hydrocarbons / Q000378:metabolism / Q000633:toxicity*; D014050:Toluene / Q000378:metabolism / Q000633:toxicity; D014992:Xylenes / Q000378:metabolism / Q000633:toxicity
D001555:Benzene Derivatives; D006838:Hydrocarbons; D014992:Xylenes; D014050:Toluene; C004912:ethylbenzene