title: Increase of foreign gene expression in monocot and dicot cells by an intron in the 5' untranslated region of a soybean phosphoenolpyruvate carboxylase gene.
abstract: A genomic clone containing part of the coding region and upstream sequences of a phosphoenolpyruvate carboxylase (PEPC) gene was isolated from a soybean genomic library. The first intron of this gene is located in the 5' untranslated region. This intron-spanning fragment is capable of increasing GUS activity in plant cells.
authors.affiliation: Mitsui Plant Biotechnology Research Institute, TCI-A1, Ibaraki, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Kato
authors.forename: E
authors.initials: E
authors.lastname: Itoh
authors.forename: R F
authors.initials: RF
authors.lastname: Whittier
authors.forename: D
authors.initials: D
authors.lastname: Shibata
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D002460:Cell Line; D018744:DNA, Plant; D015870:Gene Expression; D017343:Genes, Plant; D017930:Genes, Reporter; D005966:Glucuronidase / Q000235:genetics / Q000378:metabolism; D007438:Introns; D008969:Molecular Sequence Data; D010730:Phosphoenolpyruvate Carboxylase / Q000096:biosynthesis / Q000235:genetics*; D014176:Protein Biosynthesis; D013025:Glycine max / Q000201:enzymology* / Q000235:genetics
chemical_list: D018744:DNA, Plant; D005966:Glucuronidase; D010730:Phosphoenolpyruvate Carboxylase