Determination of cefoxitin in serum and tissue.
A simple, rapid and sensitive method for the clean-up and analysis of cefoxitin in serum and tissue is described. Serum (0.5 ml) and tissue (100 mg) samples after homogenization underwent high speed centrifugation. Chromatography was performed on a muBondapak C18 cartridge using a mobile phase of 0.005 M potassium dihydrogen phosphate-acetonitrile-glacial acetic acid (77.5:22:0.5, v/v/v) with a flow-rate of 2.0 ml/min. Ultraviolet detection occurred at 235 nm. The procedure produced a linear curve for the concentration range 100-5000 ng/ml. The assay produced accurate, repeatable and rapid results for both tissue and serum samples without the need for chemical extraction.
Cox
S K
SK
Department of Comparative Medicine, College of Veterinary Medicine, University of Tennessee, Knoxville 37901, USA.
Burnette
J D
JD
Huss
B T
BT
Frazier
D
D
D000818:Animals; D002415:Cats; D002440:Cefoxitin / Q000032:analysis / Q000097:blood*; D002513:Cephamycins / Q000032:analysis / Q000097:blood*; D002851:Chromatography, High Pressure Liquid; D003106:Colon / Q000737:chemistry*; D013056:Spectrophotometry, Ultraviolet
D002513:Cephamycins; D002440:Cefoxitin