Effect of antispermatogenic compound CDRI-84/35 on marker enzymes of rat testis cells. A study on site of action.
Marker enzymes of Sertoli and germ cells were estimated to study the mechanism of action of antispermatogenic compound CDRI 84/35 in adult male rat testis. Animals were killed after 22, 41, and 64 days of treatment with antispermatogenic dose of CDRI 84/35 in order to evaluate the effect of the compound on spermatid, spermatocyte, and spermatogonial stages, respectively. Studies were also extended to a recovery period of 90 days. Results indicate a direction action of the compound on germ cells, with no apparent effect on Sertoli cells. Studies also show a massive depletion of postmeiotic germ cells after the treatment, with some damage to premeiotic germ cells as well. Reversibility of the compound was partial, with the marker enzymes of pre- and postmeiotic germ cells not being restored to control levels after withdrawal of treatment.
Gupta
G
G
Division of Endocrinology, Central Drug Research Institute, Lucknow, India.
Maikhuri
J P
JP
Dwivedi
A K
AK
Setty
B S
BS
D000818:Animals; D000988:Antispermatogenic Agents / Q000494:pharmacology*; D015415:Biomarkers / Q000032:analysis; D015331:Cohort Studies; D005954:Glucosephosphate Dehydrogenase / Q000187:drug effects / Q000378:metabolism; D005966:Glucuronidase / Q000187:drug effects / Q000378:metabolism; D005993:Glycerolphosphate Dehydrogenase / Q000187:drug effects / Q000378:metabolism; D007064:L-Iditol 2-Dehydrogenase / Q000187:drug effects / Q000378:metabolism; D007770:L-Lactate Dehydrogenase / Q000187:drug effects / Q000378:metabolism; D008291:Malate Dehydrogenase / Q000187:drug effects / Q000378:metabolism; D008297:Male; D010879:Piperazines / Q000494:pharmacology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D013737:Testis / Q000033:anatomy & histology / Q000187:drug effects* / Q000201:enzymology; D013997:Time Factors; D005723:gamma-Glutamyltransferase / Q000187:drug effects / Q000378:metabolism
C111137:1-formyl-4-dichloroacetylpiperazine; D000988:Antispermatogenic Agents; D015415:Biomarkers; D010879:Piperazines; D005993:Glycerolphosphate Dehydrogenase; D007064:L-Iditol 2-Dehydrogenase; D007770:L-Lactate Dehydrogenase; D008291:Malate Dehydrogenase; D005954:Glucosephosphate Dehydrogenase; D005723:gamma-Glutamyltransferase; D005966:Glucuronidase