title: Sleep patterns after carbachol delivery in the ventral oral pontine tegmentum of the cat.
abstract: This study examines dose-related effects on sleep produced by low-volume and low-dose carbachol microinjections in the ventral part of the cat nucleus reticularis pontis oralis. Carbachol microinjections (0.04, 0.08, 0.8 or 4 microg; volume 20 nl) in this location triggered paradoxical sleep with a very short dose-unrelated latency. The four carbachol doses effectively generated all the polygraphic and behavioral signs of paradoxical sleep when microinjected at any level within the ventral part of the nucleus reticularis pontis oralis (AP 0.5 to -3.5, L 0.5-3.5, V 3.5-5.0, on the Reinoso-Suárez atlas [Topographischer Hirnatlas der Katze (1961); Merck, Darmstadt]). The dose-related increase of total paradoxical sleep time was due to the increase in both the duration and number of paradoxical sleep episodes. This paradoxical sleep increase was associated with a dose-related decrease in the amount of time spent in both slow wave sleep and drowsiness, but not with any decrease in total wakefulness. The lengthening of the latency to slow wave sleep onset was dose related. These results show that the ventral oral pontine tegmentum is a very sensitive site for the induction and maintenance of paradoxical sleep.
authors.affiliation: Departamento de Morfología, Facultad de Medicina, Universidad Autónoma de Madrid, Spain.
authors.forename: M
authors.initials: M
authors.lastname: Garzón
authors.forename: I
authors.initials: I
authors.lastname: De Andrés
authors.forename: F
authors.initials: F
authors.lastname: Reinoso-Suárez
mesh_terms: D000818:Animals; D001921:Brain / Q000187:drug effects / Q000502:physiology*; D001931:Brain Mapping; D002217:Carbachol / Q000008:administration & dosage / Q000494:pharmacology*; D002415:Cats; D004305:Dose-Response Relationship, Drug; D004576:Electromyography / Q000187:drug effects; D008297:Male; D008845:Microinjections; D011149:Pons / Q000187:drug effects / Q000502:physiology*; D012894:Sleep Stages / Q000187:drug effects* / Q000502:physiology; D012895:Sleep, REM / Q000187:drug effects / Q000502:physiology; D013997:Time Factors; D014851:Wakefulness
chemical_list: D002217:Carbachol