Mapping the genome of Eimeria tenella: use of a yeast artificial chromosome library as the basis for a physical map.
Efforts to derive a physical map of the genome of Eimeria tenella are being made to complement genetic mapping and to assist in the production of an integrated map. A large insert library of DNA from the Houghton (H) reference strain of E. tenella has been constructed in yeast artificial chromosomes (YACs). The library contains a tenfold E. tenella genome equivalent consisting of 3177 clones arrayed in 96-well microtitre plates and gridded on nylon membranes at 1728 clones/86 cm2. Size fractionation of a random sample of 185 YACs revealed an average insert size of approximately 170 kb. Hybridisation of four E. tenella single-copy probes to the gridded colony filters produced around four or more positive clones. A probe representing the whole of the 1.2-Mb chromosome 2, excised from a preparative gel following pulsed-field gel electrophoresis, revealed between 80 and 100 positive clones.
Hoogendoorn
B
B
Institute for Animal Health, Compton, Berkshire, UK. bastiaan.hoogendoorn@bbsrc.ac.uk
Toye
A A
AA
Bumstead
N
N
Shirley
M W
MW
D000818:Animals; D015139:Blotting, Southern; D002874:Chromosome Mapping; D018244:Chromosomes, Artificial, Yeast; D016054:DNA, Protozoan / Q000235:genetics; D016786:Eimeria tenella / Q000235:genetics*; D015723:Gene Library; D018503:Genome, Protozoan; D014170:Transformation, Genetic
D016054:DNA, Protozoan