Reverse transcription-polymerase chain reaction (RT-PCR) for direct detection of cucumber mosaic virus (CMV) in gladiolus.
A diagnostic method based on Reverse Transcription-Polymerase Chain Reaction (RT-PCR) was performed for detection of CMV genome directly in crude extracts of both healthy and infected gladiolus tissue using primers from the conserved sequences of CMV RNA-3. RT-PCR resulted in the amplification of 540 bp long fragment of CMV coat protein gene (CMV-CP), as expected in most of the plant parts of infected gladiolus samples. Positive signals in Southern hybridization of amplified fragments with cloned CMV-CP cDNA probe also confirmed the presence of CMV genome in gladiolus.
Raj
S K
SK
Plant Virus Laboratory, National Botanical Research Institute, Lucknow, India.
Saxena
S
S
Hallan
V
V
Singh
B P
BP
D000818:Animals; D015139:Blotting, Southern; D017799:Cucumovirus / Q000235:genetics / Q000302:isolation & purification* / Q000648:ultrastructure; D009693:Nucleic Acid Hybridization; D010935:Plant Diseases / Q000235:genetics* / Q000821:virology; D018515:Plant Leaves / Q000235:genetics / Q000821:virology; D018517:Plant Roots / Q000235:genetics / Q000821:virology; D018547:Plant Stems / Q000235:genetics / Q000821:virology; D010944:Plants / Q000235:genetics* / Q000821:virology*; D016133:Polymerase Chain Reaction / Q000379:methods*