Quantitative video microscopy.
Obviously there are many variations and embellishments on the topic of quantitating your image. I have tried here to offer you a very practical guide which highlights some of the critical issues paying particular attention to problems which can prevent or compromise your success. These include: errors due to aspect ratio, use of automatic camera settings, improper setting of dynamic range, and use of integer arithmetic. Further information can be found in several other chapters in this volume as well as in the references below. Additionally you shouldn't overlook the technical manuals which come with your video systems. The field of video microscopy has evolved as a cooperative effort between academia and industry. As a result you will find that many of the technical support personnel from microscope, video, and image processing companies are well versed on the issue of video imaging in biology and are more than willing to assist you.
Wolf
D E
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Department of Physiology, University of Massachusetts Medical School, Worcester 01655, USA.
D007089:Image Enhancement; D007091:Image Processing, Computer-Assisted / Q000295:instrumentation / Q000379:methods*; D018715:Microscopy, Video / Q000295:instrumentation / Q000379:methods*