title: Mucosal tenascin C content in inflammatory and neoplastic diseases of the large bowel.
abstract: OBJECTIVE
  Tenascin C is a glycoprotein of the extracellular matrix. It is upregulated during embryologic development, wound healing, and under conditions of normal and neoplastic growth. Most available data on tenascin C expression in tissues is based on immunohistologic studies. The present study was designed to quantify tissue concentrations in patients with inflammatory and neoplastic diseases of the large bowel.
  
  
  METHODS
  Fifty patients with ulcerative colitis, 19 patients suffering from familiar adenomatous polyposis without malignant transformation, and 69 patients with colorectal carcinoma were investigated. Tenascin C concentrations in tissue extracts were determined by semiquantitative Western blotting.
  
  
  RESULTS
  The tenascin C tissue concentration of normal mucosa was 2.6 +/- 3.4 microg/mg (n = 55), 2.9 +/- 2.1 microg/mg in colorectal adenomas (n = 19), 7.5 +/- 4.7 microg/mg in ulcerative colitis (n = 50), and 18 +/- 15 microg/mg in colorectal carcinomas (n = 69; mean +/- standard deviation). In ulcerative colitis, the mucosal tenascin C content correlated with histopathologic disease activity. No differences were found between subgroups of adenomas or carcinomas.
  
  
  CONCLUSIONS
  Tenascin C tissue concentrations were not altered in adenomas, slightly elevated in ulcerative colitis, and substantially increased in colorectal carcinomas. Although less useful as a diagnostic parameter, tenascin C tissue levels serve as an instrument for assessing the activity of stromal remodeling in large-bowel diseases generally. Specifically, they may reflect disease activity in ulcerative colitis.
authors.affiliation: Department of Surgery, University of Heidelberg, Germany.
authors.forename: S
authors.initials: S
authors.lastname: Riedl
authors.forename: M
authors.initials: M
authors.lastname: Kadmon
authors.forename: A
authors.initials: A
authors.lastname: Tandara
authors.forename: U
authors.initials: U
authors.lastname: Hinz
authors.forename: P
authors.initials: P
authors.lastname: Möller
authors.forename: C
authors.initials: C
authors.lastname: Herfarth
authors.forename: A
authors.initials: A
authors.lastname: Faissner
mesh_terms: D011125:Adenomatous Polyposis Coli / Q000378:metabolism; D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D015153:Blotting, Western; D002648:Child; D003093:Colitis, Ulcerative / Q000378:metabolism; D003108:Colonic Diseases / Q000378:metabolism*; D003110:Colonic Neoplasms / Q000378:metabolism*; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D007413:Intestinal Mucosa / Q000378:metabolism*; D008297:Male; D008875:Middle Aged; D019063:Tenascin / Q000378:metabolism*
chemical_list: D019063:Tenascin