Human lung fluke Paragonimus heterotremus: differential diagnosis between Paragonimus heterotremus and Paragonimus westermani infections by EITB.
The specificity of three major polypeptides (35, 33 and 32.5 kD) from Paragonimus heterotremus antigens prepared from ether-extracted adult worms was tested against sera from heterologous infections as well as against P. westermani-infected sera. Only the 35 kD polypeptide was not present, its antigenic determinant being bound to the antibodies from all P. westermani-infected cases. Its cross-reactivity against various sera from heterologous helminthiases and other lung infections showed that it is not bound to these antigenic polypeptides. These major bands cannot be detected by Concanavalin A detector. Our research encourages the pattern (35, 33 and 32.5 kD) of immunoblot reactions for the diagnosis of P. heterotremus infections; the 35 kD antigen is specific for corresponding species and able to differentiate infections between both species of Paragonimus.
Dekumyoy
P
P
Department of Helminthology, Faculty of Tropical Medicine, Mahidol University, Bangkok, Thailand.
Waikagul
J
J
Eom
K S
KS
D000818:Animals; D000909:Antibodies, Helminth / Q000276:immunology*; D000947:Antigens, Helminth / Q000276:immunology*; D003429:Cross Reactions; D003937:Diagnosis, Differential; D000939:Epitopes / Q000276:immunology; D006373:Helminthiasis / Q000175:diagnosis / Q000276:immunology; D006801:Humans; D007106:Immune Sera / Q000276:immunology; D015151:Immunoblotting; D008171:Lung Diseases / Q000175:diagnosis / Q000276:immunology; D010237:Paragonimiasis / Q000175:diagnosis* / Q000276:immunology / Q000469:parasitology; D010238:Paragonimus / Q000145:classification / Q000276:immunology*; D010455:Peptides / Q000276:immunology; D013045:Species Specificity
D000909:Antibodies, Helminth; D000947:Antigens, Helminth; D000939:Epitopes; D007106:Immune Sera; D010455:Peptides