The structure of the murine calcium channel gamma-subunit gene and protein.
The gamma-subunit of voltage-gated calcium channels is a membrane protein that is associated with the skeletal muscle type of voltage gated calcium channels. Using a subunit-specific polyclonal antibody, the gamma-protein was detected in mouse skeletal muscle but not in brain, where at least five additional types of voltage-gated calcium channels are expressed. Murine genomic clones containing the full coding sequence of the gamma-subunit were isolated, the exons were mapped and sequenced. The murine gamma-subunit is encoded by a single copy gene containing 4 translated exons which are distributed over approximately 14 kilobases of DNA. The intron placement within the mouse gene correlates with the previously revealed organization of the human gamma-subunit gene, although the primary structures of the gamma-subunits are only moderately conserved between the murine, human, rat and rabbit proteins (75% identity).
Wissenbach
U
U
Institut für Pharmakologie und Toxikologie, Universität des Saarlandes, Homburg, Germany.
Bosse-Doenecke
E
E
Freise
D
D
Ludwig
A
A
Murakami
M
M
Hofmann
F
F
Flockerzi
V
V
D017398:Alternative Splicing / Q000235:genetics; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D001923:Brain Chemistry; D015220:Calcium Channels / Q000737:chemistry* / Q000235:genetics; D003001:Cloning, Molecular; D015870:Gene Expression / Q000235:genetics; D051379:Mice; D008969:Molecular Sequence Data; D009132:Muscles / Q000737:chemistry; D011993:Recombinant Fusion Proteins / Q000235:genetics; D015183:Restriction Mapping; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D014158:Transcription, Genetic / Q000235:genetics
D015220:Calcium Channels; D011993:Recombinant Fusion Proteins