The defective antigen-presenting activity of murine fetal macrophage cell lines.
We have previously reported that placental macrophages of fetal origin have a decreased ability to present antigen. To clarify the underlying mechanism for this deficiency, we have generated primary fetal macrophage cell lines. Our data show that despite their defective antigen-presenting ability, fetal macrophages do express all known accessory molecules, intracellular adhesion molecule-1, B7 and major histocompatibility complex class II molecules. However, fetal macrophages do not express detectable invariant chain mRNA which is known to have a major role in the class II-associated antigen-processing pathway. Since fetal macrophages can neither present antigenic peptides nor superantigen, the diminished invariant chain expression alone cannot account for the impaired antigen-presenting function of fetal macrophages.
Khalili
H
H
Department of Medicine, North Shore University Hospital, New York University Medical College, Manhasset 11030, USA.
Deshpande
R
R
Chang
M Y
MY
D000818:Animals; D017951:Antigen Presentation / Q000276:immunology*; D000944:Antigens, Differentiation, B-Lymphocyte / Q000276:immunology; D018122:B7-1 Antigen / Q000378:metabolism; D018929:Cell Culture Techniques; D002460:Cell Line; D005333:Fetus / Q000276:immunology*; D000949:Histocompatibility Antigens Class II / Q000276:immunology / Q000378:metabolism; D006825:Hybridomas / Q000276:immunology; D018799:Intercellular Adhesion Molecule-1 / Q000378:metabolism; D008264:Macrophages / Q000276:immunology*; D051379:Mice; D008807:Mice, Inbred BALB C; D008809:Mice, Inbred C3H; D008815:Mice, Inbred Strains; D013601:T-Lymphocytes / Q000276:immunology
D000944:Antigens, Differentiation, B-Lymphocyte; D018122:B7-1 Antigen; D000949:Histocompatibility Antigens Class II; C045084:invariant chain; D018799:Intercellular Adhesion Molecule-1