title: Intra-assay performance characteristics of five assays for quantification of human immunodeficiency virus type 1 RNA in plasma.
abstract: Three kits (Roche AMPLICOR human immunodeficiency virus type 1 [HIV-1] Monitor, Chiron enhanced-sensitivity bDNA, and Organon Teknika NASBA HIV-1 QT) and two in-house assays (from National Genetics Institute and Baylor College of Medicine) were compared with a blinded panel. The results were evaluated as to intra-assay sensitivity, precision, and ability to detect differences in a dilution series.
authors.affiliation: Division of Molecular Virology, Baylor College of Medicine, Houston, Texas 77030, USA.
authors.forename: H J
authors.initials: HJ
authors.lastname: Lin
authors.forename: L
authors.initials: L
authors.lastname: Pedneault
authors.forename: F B
authors.initials: FB
authors.lastname: Hollinger
mesh_terms: D005069:Evaluation Studies as Topic; D005189:False Positive Reactions; D015658:HIV Infections / Q000821:virology*; D015497:HIV-1 / Q000235:genetics / Q000502:physiology*; D006801:Humans; D012367:RNA, Viral / Q000097:blood*; D011933:Reagent Kits, Diagnostic; D012015:Reference Standards; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D019562:Viral Load; D014766:Viremia
chemical_list: D012367:RNA, Viral; D011933:Reagent Kits, Diagnostic