Homocysteine redox receptor and regulation of extracellular matrix components in vascular cells.
Dynamic changes in the reduction-oxidation (redox) state of the tissue lead to the pathophysiological condition. Reduced homocysteine causes dysfunctions in endothelium. The proliferation of smooth muscle cells may lead to occlusive vascular disease, ischemia, and heart failure, but whether fibrosis and hypertension are a consequence of smooth muscle proliferation is unclear. Redox changes during hyper-homocyst(e)inemia may be one of the causes of premature atherosclerotic heart disease. To examine the effect of homocystine on human vascular smooth muscle cells (HVSMC), we isolated HVSMC from idiopathic dilated cardiomyopathic hearts. Coronaries in these hearts were apparently normal. HVSMC numbers in culture were measured by hemocytometer in the presence and absence of homocystine. Results show that homocystine induced cellular proliferation. This proliferation was reversed by the addition of the antioxidant N-acetylcysteine (NAC). Homocystine induces collagen expression in a dose- and time-dependent manner, as measured by Northern blot (mRNA) analysis. The 50% inhibitory concentration of 5 microM for collagen was estimated. The induction of collagen was reversed by the addition of NAC and reduced glutathione. To localize the receptor for homocystine on HVSMC, we synthesized fluorescamine-labeled homocystine conjugate. Incubation of labeled homocystine with HVSMC demonstrated membrane and cytosol localization of homocystine binding. The receptor-ligand binding was disrupted by NAC. Based on sodium dodecyl sulfate-polyacrylamide gel electrophoresis fluorography, we observed a 40- to 25-kDa homocystine redox receptor in HVSMC. Our results suggested that the redox homocysteine induces HVSMC proliferation by binding to the redox receptor and may exacerbate atherosclerotic lesion formation by inducing collagen expression.
Tyagi
S C
SC
Department of Physiology and Biophysics, University of Mississippi Medical Center, Jackson 39216, USA.
D000111:Acetylcysteine / Q000494:pharmacology; D000199:Actins / Q000096:biosynthesis; D000595:Amino Acid Sequence; D002455:Cell Division; D003094:Collagen / Q000096:biosynthesis; D016326:Extracellular Matrix Proteins / Q000502:physiology*; D016166:Free Radical Scavengers / Q000494:pharmacology; D005978:Glutathione / Q000378:metabolism; D006710:Homocysteine / Q000502:physiology*; D006711:Homocystine / Q000502:physiology*; D006801:Humans; D008969:Molecular Sequence Data; D009131:Muscle, Smooth, Vascular / Q000187:drug effects / Q000502:physiology*; D010084:Oxidation-Reduction; D011956:Receptors, Cell Surface / Q000187:drug effects / Q000502:physiology*
D000199:Actins; D016326:Extracellular Matrix Proteins; D016166:Free Radical Scavengers; D011956:Receptors, Cell Surface; D006710:Homocysteine; D006711:Homocystine; D003094:Collagen; D005978:Glutathione; D000111:Acetylcysteine