Rapid DNA extraction method for genetic screening.
A fast, simple, safe and inexpensive method for purification of genomic DNA from blood samples for subsequent PCR is described. All steps are carried out in a single 1.5 ml reaction tube. Neither proteinase K digestion nor precipitation steps are involved. The method is highly efficient. As little as 20 microl of whole blood gives enough DNA for reliable amplification by PCR. The blood cells are lysated in a buffer, the nuclei are pelleted and resuspended in water after a wash step. The resuspension is directly used for PCR.
Gross
M
M
Medizinische Poliklinik, Klinikum Innenstadt, Ludwig-Maximilians-Universit¿t, Pettenkoferstr. 8a, Munich, D-80336, Germany. 100606.3315@compuserve com.
Rötzer
E
E
D004247:DNA / Q000097:blood / Q000235:genetics / Q000302:isolation & purification*; D005820:Genetic Testing / Q000379:methods*; D006801:Humans; D008661:Metabolism, Inborn Errors / Q000175:diagnosis* / Q000235:genetics; D016133:Polymerase Chain Reaction
D004247:DNA