[The effect of gamma-interferon and phorbol myristate acetate on the Fc gamma-receptor ligand-binding capacity and on the target-conjugating and antibody-dependent cytotoxic activity of blood lymphocytes].
We investigated the ability of gamma-interferon (IFN-gamma) and phorbol 12-myristate 13-acetate (PMA) to influence the ligand-binding of Fc-receptors for immunoglobulin G (Fc gamma R) (conjugation of IgG to fluorescein izothiocyanate--FITC) of bovine blood lymphocytes and the antibody-dependent cell-mediated cytotoxic activity (ADCC). The results demonstrate that the IFN-gamma or PMA induced augmentation of ligand-binding capacity of Fc gamma R of bovine lymphocytes, and correlated well with the enhanced capacity to lyse L cells coated with anti-L cell antibody. The number of conjugates effector--target, formed by bovine lymphocytes in the reaction of ADCC, correlates well with ligand-binding capacity of Fc gamma R. It can be suggested that IFN-gamma or PMA may control the ADCC caused by Fc gamma R regulating its expression.
Miliukiene
V V
VV
Institute of Biochemistry, Vilnius, Lithuania.
Dediniene
J L
JL
D000276:Adjuvants, Immunologic / Q000494:pharmacology*; D000818:Animals; D000920:Antibody-Dependent Cell Cytotoxicity / Q000187:drug effects* / Q000276:immunology; D002417:Cattle; D002478:Cells, Cultured; D004305:Dose-Response Relationship, Drug; D005260:Female; D016650:Fluorescein-5-isothiocyanate; D005456:Fluorescent Dyes; D007074:Immunoglobulin G / Q000378:metabolism; D007371:Interferon-gamma / Q000494:pharmacology*; D007739:L Cells; D008024:Ligands; D008214:Lymphocytes / Q000187:drug effects* / Q000276:immunology / Q000378:metabolism; D051379:Mice; D017452:Receptors, IgG / Q000187:drug effects* / Q000276:immunology / Q000378:metabolism; D012016:Reference Values; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology*
D000276:Adjuvants, Immunologic; D005456:Fluorescent Dyes; D007074:Immunoglobulin G; D008024:Ligands; D017452:Receptors, IgG; D007371:Interferon-gamma; D016650:Fluorescein-5-isothiocyanate; D013755:Tetradecanoylphorbol Acetate
Vliianie gamma-interferona i forbolomeristicheskogo atsetata na sviazyvaiushchuiu sposobnost' Fc gamma-retseptora s ligandom, mishen'kon''iugiruiushchuiu i antitelozavisimuiu tsitotoksicheskuiu aktivnost' limfotsitov krovi.