Culture of human nasal epithelial cells from nasal polyps on collagen matrix.
Epithelial cell cultures became an experimental model employed for both animal and human studies. We established a modified method of culture of human nasal epithelial cells from nasal polyps using serum-free, hormonally supplemented Ham's F-12 medium and Vitrogen 100 collagen matrix. Cells reached confluent monolayer structures in 5 to 7 days (mean time 6 +/- 0.89 days) of culture. The confluent cultures consisted of pure epithelial cells, which was confirmed by light and electron microscopy, and immunohistochemical staining with anticytokeratin antibody. The success ratio of cultures was 61.5%. The culture system described is efficient enough to provide pure epithelial cells for further functional studies.
Kowalski
M L
ML
Department of Clinical Immunology and Allergy, University Medical School, Lódź, Poland.
Pawliczak
R
R
Woźniak
J
J
Siuda
K
K
Grzegorczyk
J
J
Pietrzak
M
M
Kozłowski
Z
Z
Marek
K
K
D018929:Cell Culture Techniques / Q000379:methods*; D002469:Cell Separation; D002478:Cells, Cultured; D003094:Collagen; D004847:Epithelial Cells / Q000166:cytology* / Q000648:ultrastructure; D006801:Humans; D007150:Immunohistochemistry; D007633:Keratins; D009298:Nasal Polyps
D007633:Keratins; D003094:Collagen