Activation by sodium fluoride of drug-metabolizing enzymes in rat hepatoma-derived Fa32 cells.
Protection against xenobiotic insult, including cancer chemoprotection, can be achieved by a variety of natural and synthetic compounds belonging to over 20 different classes of chemicals. They all induce or activate drug-metabolizing enzymes. The discovery of a new class of activator is currently reported. Sodium fluoride activated the phase I ethoxyresorufin-O-deethylase (to 240%) and pentoxyresorufin-O-depentylase (to 156%), and the phase II glutathione transferase to 120% of the basal activities in rat hepatoma-derived Fa32 cells. It is, therefore, a bifunctional enzyme activator. A time- and concentration-dependent activation was observed. A possible impact of the daily fluoride uptake from drinking water is suggested.
Dierickx
P J
PJ
Instituut voor Volksgezondheid, Afdeling Toxikologie, Brussel, Belgium. p.dierickx@iph.fgov.be
D000818:Animals; D019363:Cytochrome P-450 CYP1A1 / Q000378:metabolism*; D019362:Cytochrome P-450 CYP2B1 / Q000378:metabolism*; D004789:Enzyme Activation / Q000187:drug effects; D005459:Fluorides; D005982:Glutathione Transferase / Q000378:metabolism*; D007700:Kinetics; D008114:Liver Neoplasms, Experimental / Q000201:enzymology*; D051381:Rats; D012969:Sodium Fluoride / Q000494:pharmacology*; D014407:Tumor Cells, Cultured; D014881:Water Supply
D012969:Sodium Fluoride; D019363:Cytochrome P-450 CYP1A1; D019362:Cytochrome P-450 CYP2B1; D005982:Glutathione Transferase; D005459:Fluorides