Molecular cloning, genomic characterization and expression of novel human alpha1A-adrenoceptor isoforms.
We have isolated and characterized from human prostate novel splice variants of the human alpha1A-adrenoceptor, several of which generate truncated products and one isoform, alpha(1A-4), which has the identical splice site as the three previously described isoforms. Long-PCR on human genomic DNA showed that the alpha(1A-4) exon is located between those encoding the alpha(1A-1) and alpha(1A-3) variants. CHO-K1 cells stably expressing alpha(1A-4) showed ligand binding properties similar to those of the other functional isoforms as well as agonist-stimulated inositol phosphate accumulation. Quantitative PCR analyses revealed that alpha(1A-4) is the most abundant isoform expressed in the prostate with high levels also detected in liver and heart.
Chang
D J
DJ
Center for Biological Research, Neurobiology Unit, Roche Bioscience, Palo Alto, CA 94304, USA. david-j.chang@roche.com
Chang
T K
TK
Yamanishi
S S
SS
Salazar
F H
FH
Kosaka
A H
AH
Khare
R
R
Bhakta
S
S
Jasper
J R
JR
Shieh
I S
IS
Lesnick
J D
JD
Ford
A P
AP
Daniels
D V
DV
Eglen
R M
RM
Clarke
D E
DE
Bach
C
C
Chan
H W
HW
D000317:Adrenergic alpha-Antagonists / Q000494:pharmacology*; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D016466:CHO Cells; D003001:Cloning, Molecular; D006224:Cricetinae; D015723:Gene Library; D006801:Humans; D007295:Inositol Phosphates / Q000378:metabolism*; D008297:Male; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D011467:Prostate / Q000378:metabolism*; D012333:RNA, Messenger / Q000378:metabolism; D011869:Radioligand Assay; D018340:Receptors, Adrenergic, alpha-1 / Q000096:biosynthesis / Q000737:chemistry / Q000502:physiology*; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000737:chemistry; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid; D014162:Transfection
C547713:ADRA1A protein, human; D000317:Adrenergic alpha-Antagonists; D007295:Inositol Phosphates; D012333:RNA, Messenger; D018340:Receptors, Adrenergic, alpha-1; D011994:Recombinant Proteins