title: 5-Lipoxygenase gene expression in hemodialysis.
abstract: Leukotrienes (LTs), the end products of the eicosanoid pathway released during inflammation, are markers of polymorphonuclear cell and monocyte activation. The present study focused on the possibility that 5-lipoxygenase (5-LO), the key enzyme for LT synthesis, was involved in the interaction between blood and the hemodialysis (HD) membrane. 5-LO gene expression was examined by reverse transcriptase polymerase chain reaction (RT-PCR) in samples of mononuclear cells isolated from peripheral blood withdrawn at the start and at 15 min of HD from 10 chronic HD patients, 5 treated with Cuprophan and 5 with polymethylmethacrylate (PMMA) membrane. An increased 5-LO gene expression was detected at 15 min in 4 of 5 patients using the Cuprophan membrane but in none of the 5 PMMA treated patients. Our results showed for the first time that the interaction between blood and the HD membrane upregulates 5-LO messenger ribonucleic acid (mRNA).
authors.affiliation: Dipartimento di Medicina e Oncologia Sperimentale, Sezione di Patologia Generale, Università di Torino, Italy.
authors.forename: E
authors.initials: E
authors.lastname: Menegatti
authors.forename: D
authors.initials: D
authors.lastname: Roccatello
authors.forename: D
authors.initials: D
authors.lastname: Rossi
authors.forename: M
authors.initials: M
authors.lastname: Formica
authors.forename: G
authors.initials: G
authors.lastname: Piccoli
authors.forename: L M
authors.initials: LM
authors.lastname: Sena
mesh_terms: D000368:Aged; D000369:Aged, 80 and over; D001094:Arachidonate 5-Lipoxygenase / Q000235:genetics* / Q000378:metabolism; D002482:Cellulose / Q000031:analogs & derivatives / Q000737:chemistry / Q000378:metabolism; D005260:Female; D015971:Gene Expression Regulation, Enzymologic / Q000235:genetics*; D006801:Humans; D007963:Leukocytes, Mononuclear / Q000201:enzymology; D015289:Leukotrienes / Q000096:biosynthesis; D008297:Male; D008567:Membranes, Artificial; D008875:Middle Aged; D016133:Polymerase Chain Reaction; D019904:Polymethyl Methacrylate / Q000737:chemistry / Q000378:metabolism; D012333:RNA, Messenger / Q000378:metabolism; D006435:Renal Dialysis; D051437:Renal Insufficiency / Q000628:therapy; D015854:Up-Regulation / Q000235:genetics
chemical_list: D015289:Leukotrienes; D008567:Membranes, Artificial; D012333:RNA, Messenger; D002482:Cellulose; D019904:Polymethyl Methacrylate; C009865:cuprammonium cellulose; D001094:Arachidonate 5-Lipoxygenase