[Interaction of phenolic antioxidants and 1,4-dihydropyridines with membranes of rat liver microsome fractions].
Interaction of phenol type antioxidants and Ca-channel blockers, derivatives of 1,4-dihydropyridines, with rat liver microsomes has been studied in vitro by means of spectrophotometry and fluorescent probes using ANS and polymethine dye 4501 u. Incubation of microsomes in the absorption spectra of the antioxidant results in changes of structure dynamic parametres of microsomal membranes, and in changes of their microviscosity and in surface state modification. The mechanism of microsomal action was different for phenol type antioxidants and for 1,4-dihydropyridines and depended on the radical structure. Ability of Ca-channel blockers and verapamil to interact with microsomal membrane protein structures was shown.
Gubskiĭ
Iu I
IuI
Institute of Pharmacology and Toxicology, Academy of Medical Sciences of Ukraine, Kyiv.
Goriushko
A G
AG
Shnurko-Tabakova
E V
EV
D000818:Animals; D000975:Antioxidants / Q000494:pharmacology*; D002121:Calcium Channel Blockers / Q000494:pharmacology*; D004095:Dihydropyridines / Q000494:pharmacology*; D066298:In Vitro Techniques; D007425:Intracellular Membranes / Q000187:drug effects*; D008862:Microsomes, Liver / Q000187:drug effects* / Q000648:ultrastructure; D010636:Phenols / Q000494:pharmacology*; D051381:Rats; D013056:Spectrophotometry, Ultraviolet
D000975:Antioxidants; D002121:Calcium Channel Blockers; D004095:Dihydropyridines; D010636:Phenols
Izuchenie vzaimodeĭstviia fenol'nykh antioksidantov i 1,4-digidropiridinov s membranami fraktsiĭ mikrosom pecheni krys.