Inhibition of forskolin-induced sensitisation of frog skin nociceptors by the cyclic AMP-dependent protein kinase A antagonist H-89.
The role of cAMP and protein kinase A (PKA) in nociceptor sensitisation has been studied in frog skin in vitro. Multifibre nerve responses during noxious heating were enhanced by adding forskolin, an agent that elevates cAMP. H-89, a PKA antagonist, blocked the increased responses due to forskolin, but did not affect baseline responses. Thus, cAMP appears to act via PKA when sensitising nociceptors in frog skin.
Lynn
B
B
Department of Physiology, University College London, UK. b.lynn@ucl.ac.uk
O'Shea
N R
NR
D000200:Action Potentials / Q000187:drug effects / Q000502:physiology; D000818:Animals; D005576:Colforsin / Q000494:pharmacology*; D000242:Cyclic AMP / Q000378:metabolism; D017868:Cyclic AMP-Dependent Protein Kinases / Q000037:antagonists & inhibitors*; D004791:Enzyme Inhibitors / Q000494:pharmacology*; D006358:Hot Temperature; D006930:Hyperalgesia / Q000378:metabolism; D007546:Isoquinolines / Q000494:pharmacology*; D009475:Neurons, Afferent / Q000187:drug effects / Q000201:enzymology; D009619:Nociceptors / Q000187:drug effects*; D011894:Rana pipiens; D012867:Skin / Q000294:innervation; D013449:Sulfonamides
D004791:Enzyme Inhibitors; D007546:Isoquinolines; D013449:Sulfonamides; D005576:Colforsin; D000242:Cyclic AMP; D017868:Cyclic AMP-Dependent Protein Kinases; C063509:N-(2-(4-bromocinnamylamino)ethyl)-5-isoquinolinesulfonamide