title: [Millemetre waves inhibit the synergistic effect of calcium ionophore A23187 and phorbol ester in neutrophil respiratory burst].
abstract: The effect of extremely high frequency electromagnetic field (mm-waves) on respiratory burst of neutrophils was studied. The peritoneal evoked neutrophils of the mice (NMRI line) were used. The production of reactive oxygen species was estimated by luminol-dependent chemiluminescence technique. Cells were irradiated by the mm-waves of 41. 95 GHz in the far field zone of the channel radiator during 20 min. Absorbed energy flux density was 150 microW/cm2. The irradiation was carried out at different concentrations of calcium ionophore A23187 and then neutrophils were stimulated by phorbol 12-myristate 13-acetate (PMA) 1 microM, activator of PKC. At irradiation of neutrophils the synergistic action of A23187 and PMA was not changed at low concentration of ionophore 10 nM-0.5 microM and was suppressed at high concentrations 0.5-10 microM. The largest inhibition of about 60% was obtained at the concentration of A23187 20 microM. The effect of mm-waves was not found under exposure in Ca(2+)-free medium for all used A23187 concentrations. We suggest that the mm-wave effect on the production of reactive oxygen species by neutrophils is determined by the influx of extracellular Ca2+ and PKC.
authors.affiliation: Institute of Cell Biophysics, Russian Acad. Sci., Pushchino, Moscow Region.
authors.forename: V G
authors.initials: VG
authors.lastname: Safronova
authors.forename: A B
authors.initials: AB
authors.lastname: Gapeev
authors.forename: A A
authors.initials: AA
authors.lastname: Alovskaia
authors.forename: A G
authors.initials: AG
authors.lastname: Gabdulkhakova
authors.forename: N K
authors.initials: NK
authors.lastname: Chemeris
authors.forename: E E
authors.initials: EE
authors.lastname: Fesenko
mesh_terms: D000818:Animals; D000001:Calcimycin / Q000494:pharmacology*; D004357:Drug Synergism; D004574:Electromagnetic Fields; D007476:Ionophores / Q000494:pharmacology*; D051379:Mice; D009504:Neutrophils / Q000187:drug effects* / Q000528:radiation effects*; D016897:Respiratory Burst / Q000187:drug effects* / Q000528:radiation effects*; D015398:Signal Transduction / Q000187:drug effects* / Q000528:radiation effects*; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology*
chemical_list: D007476:Ionophores; D000001:Calcimycin; D013755:Tetradecanoylphorbol Acetate
vernacular_title: Millimetrovye volny ingibiruiut sinergicheskiĭ effekt kal'tsievogo ionofora A23187 i forbolovogo efira v aktivatsii respiratornogo vzryva neĭtrofilov.