title: Screening of larval/pupal P-element induced lethals on the second chromosome in Drosophila melanogaster: clonal analysis and morphology of imaginal discs.
abstract: We have carried out screens for lethal mutations on the second chromosome of Drosophila melanogaster that are associated with abnormal imaginal disc morphologies, particularly in the wing disc. From a collection of 164 P element-induced mutations with a late larva/pupa lethal phase we have identified 56 new loci whose gene products are required for normal wing disc development and for normal morphology of other larval organs. Genetic mosaics of these 56 mutant lines show clonal mutant phenotypes for 23 cell-viable mutations. These phenotypes result from altered cell parameters. Causal relationships between disc and clonal phenotypes are discussed.
authors.affiliation: Centro de Biología Molecular Severo Ochoa, Madrid, Spain.
authors.forename: F
authors.initials: F
authors.lastname: Roch
authors.forename: F
authors.initials: F
authors.lastname: Serras
authors.forename: F J
authors.initials: FJ
authors.lastname: Cifuentes
authors.forename: M
authors.initials: M
authors.lastname: Corominas
authors.forename: B
authors.initials: B
authors.lastname: Alsina
authors.forename: M
authors.initials: M
authors.lastname: Amorós
authors.forename: A
authors.initials: A
authors.lastname: López-Varea
authors.forename: R
authors.initials: R
authors.lastname: Hernández
authors.forename: D
authors.initials: D
authors.lastname: Guerra
authors.forename: S
authors.initials: S
authors.lastname: Cavicchi
authors.forename: J
authors.initials: J
authors.lastname: Baguñá
authors.forename: A
authors.initials: A
authors.lastname: García-Bellido
mesh_terms: D000818:Animals; D002874:Chromosome Mapping; D002999:Clone Cells; D004251:DNA Transposable Elements / Q000235:genetics*; D004331:Drosophila melanogaster / Q000235:genetics* / Q000254:growth & development; D004847:Epithelial Cells / Q000473:pathology; D018507:Gene Expression Regulation, Developmental; D017344:Genes, Insect; D005804:Genes, Lethal; D007814:Larva; D009024:Morphogenesis / Q000235:genetics; D009030:Mosaicism; D016254:Mutagenesis, Insertional; D010641:Phenotype; D011679:Pupa; D014921:Wings, Animal / Q000002:abnormalities / Q000254:growth & development*
chemical_list: D004251:DNA Transposable Elements