Neurotactin functions in concert with other identified CAMs in growth cone guidance in Drosophila.
We have isolated and characterized mutations in Drosophila neurotactin, a gene that encodes a cell adhesion protein widely expressed during neural development. Analysis of both loss and gain of gene function conditions during embryonic and postembryonic development revealed specific requirements for neurotactin during axon outgrowth, fasciculation, and guidance. Furthermore, embryos of some double mutant combinations of neurotactin and other genes encoding adhesion/signaling molecules, including neuroglian, derailed, and kekkon1, displayed phenotypic synergy. This result provides evidence for functional cooperativity in vivo between the adhesion and signaling pathways controlled by neurotactin and the other three genes.
Speicher
S
S
Centro de Biología Molecular Severo Ochoa (C.S.I.C.-U.A.M.), Universidad Autónoma, Madrid, Spain.
García-Alonso
L
L
Carmena
A
A
Martín-Bermudo
M D
MD
de la Escalera
S
S
Jiménez
F
F
D000483:Alleles; D000818:Animals; D015815:Cell Adhesion Molecules / Q000235:genetics* / Q000378:metabolism; D015816:Cell Adhesion Molecules, Neuronal / Q000235:genetics / Q000378:metabolism; D002490:Central Nervous System / Q000166:cytology; D004330:Drosophila / Q000235:genetics*; D029721:Drosophila Proteins; D018507:Gene Expression Regulation, Developmental; D019476:Insect Proteins / Q000235:genetics / Q000378:metabolism; D008562:Membrane Glycoproteins / Q000235:genetics* / Q000378:metabolism; D008565:Membrane Proteins / Q000235:genetics / Q000378:metabolism; D016296:Mutagenesis; D009419:Nerve Tissue Proteins / Q000235:genetics / Q000378:metabolism; D016501:Neurites / Q000502:physiology*; D009475:Neurons, Afferent / Q000737:chemistry / Q000201:enzymology / Q000648:ultrastructure; D010641:Phenotype; D017027:Protein Tyrosine Phosphatases; D020794:Receptor Protein-Tyrosine Kinases / Q000235:genetics / Q000378:metabolism
D015815:Cell Adhesion Molecules; D015816:Cell Adhesion Molecules, Neuronal; D029721:Drosophila Proteins; D019476:Insect Proteins; D008562:Membrane Glycoproteins; D008565:Membrane Proteins; D009419:Nerve Tissue Proteins; C066176:Nrt protein, Drosophila; C061745:Nrg protein, Drosophila; C094492:DRL protein, Drosophila; D020794:Receptor Protein-Tyrosine Kinases; C105053:Kek1 protein, Drosophila; D017027:Protein Tyrosine Phosphatases