title: Macromolecular matchmaking: advances in two-hybrid and related technologies.
abstract: The success of the original yeast two-hybrid system has stimulated the development of a number of 'hybrid technologies' in yeast (and now prokaryotes and mammals) to widen the scope of the protein-protein interactions that can be analyzed, and to enable comparable studies of the interactions of proteins with DNA, RNA or small chemical ligands. In addition, the application of the two-hybrid system to entire genomes is being used to create protein linkage maps which catalog the network of interactions of an organism's complete proteome.
authors.affiliation: Howard Hughes Medical Institute, University of Washington, Seattle 98195, USA. rfreder@genetics.washington.edu
authors.forename: R M
authors.initials: RM
authors.lastname: Frederickson
mesh_terms: D001671:Biochemistry / Q000379:methods*; D005656:Fungal Proteins / Q000235:genetics*; D006822:Hybrid Cells / Q000502:physiology*; D046911:Macromolecular Substances; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism*; D015003:Yeasts / Q000235:genetics / Q000378:metabolism
chemical_list: D005656:Fungal Proteins; D046911:Macromolecular Substances; D011994:Recombinant Proteins