Gene expression of gonadotropin-releasing hormone, but not its receptor, in human endometrium and decidua.
Gonadotropin-releasing hormone (GnRH) has been reported to exist in extrahypothalamic tissues such as the placenta, gonads and mammary glands. While we have reported the presence of GnRH-mRNA in the rodent uterus, there have been no reports concerning gene expression of GnRH and its receptor (GnRH-R) in human endometrial tissue. In order to investigate the role of GnRH as a local regulator in the human endometrium, we examined the gene for GnRH and GnRH-R in non-pregnant endometrium and decidua of early pregnancy. Using reverse transcriptase-polymerase chain reaction (RT-PCR) and Southern blot analysis we found GnRH-mRNA but not GnRH-R-mRNA transcripts in the human endometrium and decidua at 7-9 weeks gestation. This is the first report that suggests GnRH gene expression in the human endometrium/decidua.
Ikeda
M
M
Department of Obstetrics and Gynecology, Yokohama City University School of Medicine, Yokohama, Japan.
Taga
M
M
Kurogi
K
K
Minaguchi
H
H
D015139:Blotting, Southern; D003656:Decidua / Q000737:chemistry / Q000378:metabolism*; D004717:Endometrium / Q000737:chemistry / Q000378:metabolism*; D005260:Female; D015870:Gene Expression / Q000235:genetics; D005865:Gestational Age; D007987:Gonadotropin-Releasing Hormone / Q000235:genetics*; D006801:Humans; D016133:Polymerase Chain Reaction; D011247:Pregnancy; D011966:Receptors, LHRH / Q000235:genetics*
D011966:Receptors, LHRH; D007987:Gonadotropin-Releasing Hormone