Whole-body composition of Xenopus laevis larvae: implications for lean body mass during development.
Body composition in developing animals has been extensively investigated in fish larvae and bird embryos. However, no studies to date have attempted to determine whole-animal body composition or lean body mass (MLB) in developing amphibians. The present study investigates how body composition changes during development in Xenopus laevis and the potential implications of MLB for substrate turnover, energy stores, oxygen consumption and other physiological measures. Whole-animal composition was determined during development from eggs (NF stage 1) to 2 weeks post-feeding (NF 50-51), which represents two-thirds of the developmental period. Wet and dry masses were found to be highly correlated, with water content remaining constant at 93 % of wet mass. Whole-animal nucleic acid content was linearly correlated with both wet and dry masses, and declined relative to mass as development progressed. Similarly, total protein content was linearly correlated with wet and dry masses; however, total protein content increased with developmental stage. Amounts of individual neutral lipids were variable although, overall, total neutral lipid content declined progressively with development. The stoichiometric energy balance paralleled the changes seen in mass-specific .MO2, with the energy primarily from lipids fueling respiration up to NF 44-45. Quantification of total body composition revealed that lipid stores greatly influenced the calculations of MLB and therefore had profound underestimating effects on the mass-specific expression of numerous physiological measures through development.
Territo
P R
PR
Department of Biological Sciences, University of Nevada at Las Vegas, Las Vegas, NV 89154-4004, USA. territop@zeus.nhlbi.nih.gov
Smits
A W
AW
D000818:Animals; D001823:Body Composition; D001834:Body Water / Q000378:metabolism; D001835:Body Weight; D004247:DNA / Q000378:metabolism; D004734:Energy Metabolism; D007814:Larva / Q000033:anatomy & histology / Q000254:growth & development / Q000378:metabolism; D050356:Lipid Metabolism; D011506:Proteins / Q000378:metabolism; D014982:Xenopus laevis / Q000033:anatomy & histology / Q000254:growth & development* / Q000378:metabolism*
D011506:Proteins; D004247:DNA