Sequences of Saccharomyces cerevisiae 2 microns DNA improving plasmid partitioning in Hansenula polymorpha.
Insertion of the HindIII-PstI fragment of Saccharomyces cerevisiae 2 microns DNA into the Hansenula polymorpha replicative plasmids decreases plasmid copy number and ensures their distribution to daughter cells at both mitotic and meiotic cell divisions. This suggests that the stabilization effect is caused by the improvement of plasmid partitioning. Deletion analysis revealed that the region of 2 microns DNA sequence responsible for the increase of mitotic stability of H. polymorpha plasmids involves the 2 microns STB locus and adjoining region. Further analysis demonstrated that the stabilization effect may depend on the number of 24-28 bp imperfect repeats which were found in several copies in the STB locus and adjoining region.
Bogdanova
A I
AI
Institute of Experimental Cardiology, Cardiology Research Center, Moscow, Russia.
Kustikova
O S
OS
Agaphonov
M O
MO
Ter-Avanesyan
M D
MD
D001483:Base Sequence; D004271:DNA, Fungal / Q000378:metabolism; D015247:Deoxyribonuclease HindIII / Q000378:metabolism; D015252:Deoxyribonucleases, Type II Site-Specific / Q000378:metabolism; D008540:Meiosis; D008938:Mitosis; D010843:Pichia / Q000235:genetics* / Q000254:growth & development; D010957:Plasmids; D012091:Repetitive Sequences, Nucleic Acid; D015183:Restriction Mapping; D012441:Saccharomyces cerevisiae / Q000235:genetics* / Q000254:growth & development; D017422:Sequence Analysis, DNA; D017384:Sequence Deletion; D014170:Transformation, Genetic
D004271:DNA, Fungal; D015247:Deoxyribonuclease HindIII; C024388:CTGCAG-specific type II deoxyribonucleases; D015252:Deoxyribonucleases, Type II Site-Specific