Characterization of the bovine epsilon gene.
Immunoglobulin E is quantitatively a minor immunoglobulin class in serum, but nevertheless the major class of antibody mediating type I hypersensitivity reactions and hence, type I allergic phenomena. The bovine epsilon gene is one of the as yet uncharacterized mammalian immunoglobulin genes. We have therefore cloned and determined the cDNA sequence and genomic organization of the gene. It contains four constant region domain-encoding exons (CH1 to CH4) with a high homology to sheep C epsilon (87%) and to a lower degree to dog (62%), horse (58%), chimpanzee, orangutan, human (55%), mouse (52%) and rat (52%) C epsilon genes. Southern blot analysis of bovine genomic DNA, revealed the existence of a single C epsilon gene with a presence of allelic restriction fragment length polymorphism (RFLP).
Mousavi
M
M
Department of Biosciences at NOVUM, Karolinska Institute, Huddinge, Sweden.
Rabbani
H
H
Hammarström
L
L
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D015139:Blotting, Southern; D002417:Cattle / Q000235:genetics* / Q000276:immunology; D005803:Genes, Immunoglobulin; D007127:Immunoglobulin Constant Regions / Q000235:genetics; D007073:Immunoglobulin E / Q000235:genetics*; D007139:Immunoglobulin epsilon-Chains / Q000235:genetics; D008322:Mammals / Q000235:genetics / Q000276:immunology; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D013045:Species Specificity
D007127:Immunoglobulin Constant Regions; D007139:Immunoglobulin epsilon-Chains; D007073:Immunoglobulin E