Effect of glutaraldehyde on hemoglobin-dependent quenching of pyrene fluorescence. Application to oxygen diffusion in erythrocyte.
In the present study, we investigate the effect of glutaraldehyde incorporation on the erythrocyte deformability, membrane fluidity and process of molecular oxygen diffusion. The erythrocyte deformability variations were inversely related with the glutaraldehyde concentration incorporated. The membrane fluidity, as assessed by a method based on the kinetics of pyrene dodecanoic acid excimers formation, decreased as the glutaraldehyde concentration increased. So, we verified that glutaraldehyde incorporation was accompanied by membrane rigidification. In the presence of glutaraldehyde, decreased absorption of hemoglobin heme group (420 nm) and decreased hemoglobin-dependent quenching of pyrene butyric acid (PBA) fluorescence would result from hemoglobin polymerization. Using a technique of fluorescence intensity quenching by molecular oxygen (accessibility to oxygen), we showed that oxygen diffusion was decreased in the presence of glutaraldehyde. To conclude, membrane rigidification induced by glutaraldehyde incorporation would affect oxygen molecular process.
Dumas
D
D
Laboratoire d'Hémorhéologie-Angiohématologie, EA 1728, Faculté de Médecine, Vandoeuvre-lès-Nancy, France. Dumas@hemato.u-nancy.fr
Gouin
F
F
Viriot
M L
ML
Stoltz
J F
JF
D002087:Butyrates / Q000494:pharmacology; D020148:Butyric Acid; D004058:Diffusion; D004907:Erythrocyte Deformability / Q000187:drug effects; D004912:Erythrocytes / Q000187:drug effects; D005453:Fluorescence; D005976:Glutaral / Q000494:pharmacology*; D006454:Hemoglobins / Q000187:drug effects*; D006801:Humans; D008560:Membrane Fluidity / Q000187:drug effects; D010100:Oxygen / Q000493:pharmacokinetics; D011721:Pyrenes / Q000737:chemistry*; D013057:Spectrum Analysis
D002087:Butyrates; D006454:Hemoglobins; D011721:Pyrenes; D020148:Butyric Acid; C030984:pyrene; D010100:Oxygen; D005976:Glutaral