title: Corticotropin-releasing hormone expression in supraoptic neurons after bilateral lesioning of the paraventricular nucleus in rats.
abstract: In situ hybridization histochemistry was used to demonstrate corticotropin releasing hormone (CRH) mRNA expression in the supraoptic nucleus of rats. Labeled cells with a range of grain densities were located mainly in the dorsal area of the nucleus. Long-term (6-weeks) lesioning of the hypothalamic paraventricular nucleus, which eliminates the major CRH pool from the hypothalamo-hypophyseal system resulted in an increased CRH mRNA density within supraoptic neurons compared to within sham-operated rats. Adrenalectomy failed to effect CRH mRNA content either in sham-operated or paraventricular-lesioned animals. CRH gene expression in supraoptic neurons of long-term paraventricular lesioned rats may exhibit a compensatory mechanism in the hypothalamus by which supraoptic neurons can take over some of the functions of the lesioned paraventricular CRH cells.
authors.affiliation: Laboratory of Neuromorphology, Semmelweis University Medical School, Budapest, Hungary.
authors.forename: M
authors.initials: M
authors.lastname: Palkovits
authors.forename: K
authors.initials: K
authors.lastname: Kovács
authors.forename: W S
authors.initials: WS
authors.lastname: Young
authors.forename: G B
authors.initials: GB
authors.lastname: Makara
mesh_terms: D000324:Adrenocorticotropic Hormone / Q000097:blood; D000818:Animals; D003345:Corticosterone / Q000097:blood; D003346:Corticotropin-Releasing Hormone / Q000096:biosynthesis* / Q000235:genetics; D005786:Gene Expression Regulation / Q000187:drug effects; D006651:Histocytochemistry; D017403:In Situ Hybridization; D008297:Male; D009474:Neurons / Q000378:metabolism*; D010286:Paraventricular Hypothalamic Nucleus / Q000502:physiology* / Q000601:surgery; D012333:RNA, Messenger / Q000096:biosynthesis; D051381:Rats; D011919:Rats, Inbred Strains; D013238:Stereotaxic Techniques; D013495:Supraoptic Nucleus / Q000378:metabolism*
chemical_list: D012333:RNA, Messenger; D000324:Adrenocorticotropic Hormone; D003346:Corticotropin-Releasing Hormone; D003345:Corticosterone