Evaluation of the activities of eight lysosomal hydrolases in sera of humans, rats and pigs of different ages.
The activities of 21 enzymes (belonging to four classes of enzymes) involved in different metabolic pathways were assayed in blood sera of healthy young and adult/elderly groups of humans, rats and pigs, to determine whether activity changes coinciding with changes in age and aging could be detected. In all three species analysed, measurable activities (performed by highly specific and sensitive techniques, generally spectrofluorimetric procedures) were found, usually following a decreasing order of: among glycosidases, beta-N-acetylglucosaminidase (NAG) > alpha-L-fucosidase > alpha-mannosidase > beta-glucuronidase > beta-galactosidase > alpha-galactosidase. In addition, among esterases very high values were found for arylesterase and acid phosphatase. By contrast, no measurable activity was found for the remaining enzymes assayed (8 hydrolases, 1 oxidoreductase, 3 transferases and 1 lyase). In the elderly group of humans, significantly higher activities (P < or = 0.05) were found for NAG, alpha-mannosidase and beta-glucuronidase in comparison to the adult and young groups. However, several activities in rats and all activities in pigs decreased with age. In conclusion, differences in the activities of 6 lysosomal glycosidases and 2 esterases (but no significant differences for another 13 enzymes belonging to several enzyme classes) are found in the sera of healthy humans, rats and pigs. These differences coincide with changes observed in aging.
Sánchez-Martín
M M
MM
Departamento de Bioquímica y Biología Molecular, Facultad de Biología, Salamanca, Spain.
Cabezas
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JA
D000135:Acid Phosphatase / Q000378:metabolism; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000375:Aging / Q000378:metabolism*; D000818:Animals; D001798:Blood Proteins / Q000032:analysis; D002265:Carboxylic Ester Hydrolases / Q000378:metabolism; D002648:Child; D005260:Female; D006026:Glycoside Hydrolases / Q000378:metabolism; D006801:Humans; D006867:Hydrolases / Q000378:metabolism*; D008247:Lysosomes / Q000201:enzymology*; D008297:Male; D008875:Middle Aged; D051381:Rats; D017208:Rats, Wistar; D013552:Swine
D001798:Blood Proteins; D006867:Hydrolases; D002265:Carboxylic Ester Hydrolases; C019734:arylesterase; D000135:Acid Phosphatase; D006026:Glycoside Hydrolases