Alternative splicing of the glutamate transporter EAAT2 (GLT-1).
The human glutamate transporter EAAT2 (GLT-1) is of major importance for synaptic glutamate reuptake, and reportedly, a candidate gene for neurodegenerative diseases such as amyotrophic lateral sclerosis, Alzheimer's disease and epilepsy. Here we report the polymerase chain reaction (PCR) cloning of two novel EAAT2 transcripts, named EAAT2-C1 and EAAT2-C2, which originate from alternative splicing of the human EAAT2 gene. EAAT2-C1 results from skipping of the protein coding exon eight. In contrast, EAAT2-C2 is characterized by usage of internal splice sites in the exons five and six. The splicing events lead to a deletion of 45 and 107 amino acids, respectively, located in the C-terminal and central part of the putative protein.
Meyer
T
T
Department of Neurology, University of Ulm, Germany. thomas.meyer@medizin.uni-ulm.de
Münch
C
C
Knappenberger
B
B
Liebau
S
S
Völkel
H
H
Ludolph
A C
AC
D018528:ATP-Binding Cassette Transporters / Q000235:genetics*; D017398:Alternative Splicing / Q000235:genetics*; D027322:Amino Acid Transport System X-AG; D001921:Brain; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000302:isolation & purification; D027342:Excitatory Amino Acid Transporter 2; D006801:Humans; D017981:Receptors, Neurotransmitter / Q000235:genetics*; D017384:Sequence Deletion
D018528:ATP-Binding Cassette Transporters; D027322:Amino Acid Transport System X-AG; D018076:DNA, Complementary; D027342:Excitatory Amino Acid Transporter 2; D017981:Receptors, Neurotransmitter