In vitro cultivation of Cryptosporidium parvum in the non-adherent human monocytic THP-1 cell line.
This study shows that the human monocytic cell line THP-1 supports the growth of C. parvum. Immunofluorescence controls showed that only scarce oocysts remained after infection and disappeared within the first 24 h of culture. A continuous asexual life cycle proceeded throughout the experiments, with at least 15-d cultures. This model provides a useful tool for studies on the biology of C. parvum in cells involved in its transport in immunocompromised host.
Lawton
P
P
Lab. Parasitologie, Fac. Pharm., Univ. Claude Bernard, Lyon, France.
Naciri
M
M
Mancassola
R
R
Petavy
A F
AF
D000818:Animals; D002448:Cell Adhesion; D002460:Cell Line; D016785:Cryptosporidium parvum / Q000254:growth & development*; D006801:Humans; D009000:Monocytes / Q000166:cytology / Q000469:parasitology*