Monocyte chemoattractant protein-1 enhances expression of intercellular adhesion molecule-1 following ischemia-reperfusion of the liver in rats.
Intercellular adhesion molecule-1 (ICAM-1) is important in neutrophil-dependent injury. We investigated the effects of monocyte chemoattractant protein-1 (MCP-1) produced by Kupffer cells on ICAM-1 expression after ischemia-reperfusion in rat liver by occluding the portal vein for 30 minutes. Serum concentrations of MCP-1 increased persistently. By Northern analysis, MCP-1 mRNA increased early and persisted. Kupffer cells harvested 6 hours after reperfusion also expressed this transcript. The transcript and protein also were produced by Kupffer cells from naive controls in response to reactive oxygen species. ICAM-1 mRNA transcripts increased, peaked 3 hours after reperfusion, and decreased gradually thereafter. The level of ICAM-1 mRNA transcripts in the WK-5 rat endothelial cell line were markedly enhanced by MCP-1. These results suggest that MCP-1 released by Kupffer cells early after ischemia-reperfusion modulates neutrophil-dependent tissue injury via ICAM-1.
Yamaguchi
Y
Y
Department of Surgery II, Kumamoto University Medical School, Japan.
Matsumura
F
F
Takeya
M
M
Ichiguchi
O
O
Kuratsu
J I
JI
Horiuchi
T
T
Akizuki
E
E
Matsuda
T
T
Okabe
K
K
Ohshiro
H
H
Liang
J
J
Mori
K
K
Yamada
S
S
Takahashi
K
K
Ogawa
M
M
D000818:Animals; D002478:Cells, Cultured; D018932:Chemokine CCL2 / Q000502:physiology*; D018799:Intercellular Adhesion Molecule-1 / Q000096:biosynthesis* / Q000235:genetics; D007511:Ischemia / Q000378:metabolism*; D008099:Liver / Q000098:blood supply*; D008297:Male; D012333:RNA, Messenger / Q000032:analysis; D051381:Rats; D017208:Rats, Wistar; D015424:Reperfusion
D018932:Chemokine CCL2; D012333:RNA, Messenger; D018799:Intercellular Adhesion Molecule-1