title: Lipoxygenase products induce ultraweak light emission from human erythroleukemia cells.
abstract: Several lipoxygenase products were able to enhance ultraweak light emission and membrane permeability of human erythroleukemia K562 cells. In particular, 13-hydroperoxyoctadecadienoic acid (13-HPOD) was more effective than hydrophilic hydroperoxides, like H2O2 and tert-butyl hydroperoxide. The enhancement of luminescence induced by 13-HPOD was inhibited by superoxide and hydroxyl radical scavengers. The effect of 13-HPOD was inhibited by superoxide and hydroxyl radical scavengers. The effect of 13-HPOD was potentiated by the calcium ionophore A23187 and inhibited by the calcium chelator EDTA, and was observed also in liposomes containing unsaturated lipids. Cholesterol enrichment, which decreases the membrane fluidity, did not modify the effect of 13-HPOD on K562 cells.
authors.affiliation: Department of Experimental Medicine and Biochemical Sciences, University of Rome, Tor Vergata, Italy.
authors.forename: M
authors.initials: M
authors.lastname: Maccarrone
authors.forename: N
authors.initials: N
authors.lastname: Rosato
authors.forename: A
authors.initials: A
authors.lastname: Finazzi Agrò
mesh_terms: D000001:Calcimycin / Q000494:pharmacology; D002460:Cell Line; D002463:Cell Membrane Permeability / Q000187:drug effects / Q000502:physiology; D002784:Cholesterol / Q000494:pharmacology; D004492:Edetic Acid / Q000494:pharmacology; D016166:Free Radical Scavengers / Q000494:pharmacology; D006801:Humans; D006861:Hydrogen Peroxide / Q000494:pharmacology; D004915:Leukemia, Erythroblastic, Acute / Q000503:physiopathology*; D015289:Leukotrienes / Q000494:pharmacology; D008027:Light; D008041:Linoleic Acids / Q000494:pharmacology*; D008054:Lipid Peroxides / Q000494:pharmacology; D008081:Liposomes; D008084:Lipoxygenase / Q000378:metabolism; D008163:Luminescent Measurements; D008560:Membrane Fluidity / Q000187:drug effects; D010545:Peroxides / Q000494:pharmacology; D014407:Tumor Cells, Cultured; D020122:tert-Butylhydroperoxide
chemical_list: D016166:Free Radical Scavengers; D015289:Leukotrienes; D008041:Linoleic Acids; D008054:Lipid Peroxides; D008081:Liposomes; D010545:Peroxides; C038649:13-hydroperoxy-9,11-octadecadienoic acid; D000001:Calcimycin; C025086:15-hydroperoxy-5,8,11,13-eicosatetraenoic acid; D020122:tert-Butylhydroperoxide; D002784:Cholesterol; D004492:Edetic Acid; D006861:Hydrogen Peroxide; D008084:Lipoxygenase