title: In vitro cultivation of Cryptosporidium parvum in the non-adherent human monocytic THP-1 cell line.
abstract: This study shows that the human monocytic cell line THP-1 supports the growth of C. parvum. Immunofluorescence controls showed that only scarce oocysts remained after infection and disappeared within the first 24 h of culture. A continuous asexual life cycle proceeded throughout the experiments, with at least 15-d cultures. This model provides a useful tool for studies on the biology of C. parvum in cells involved in its transport in immunocompromised host.
authors.affiliation: Lab. Parasitologie, Fac. Pharm., Univ. Claude Bernard, Lyon, France.
authors.forename: P
authors.initials: P
authors.lastname: Lawton
authors.forename: M
authors.initials: M
authors.lastname: Naciri
authors.forename: R
authors.initials: R
authors.lastname: Mancassola
authors.forename: A F
authors.initials: AF
authors.lastname: Petavy
mesh_terms: D000818:Animals; D002448:Cell Adhesion; D002460:Cell Line; D016785:Cryptosporidium parvum / Q000254:growth & development*; D006801:Humans; D009000:Monocytes / Q000166:cytology / Q000469:parasitology*