Transgene expression in rat fetal brain grafts is maintained for 7 months after ex vivo adenoviral vector-mediated gene transfer.
Fetal brain tissue fragments containing the suprachiasmatic nucleus were infected with an adenoviral vector containing the marker gene LacZ encoding for beta-galactosidase, and subsequently cultured or transplanted in the third ventricle of SCN-lesioned adult Wistar rats. In previous studies we optimized the infection procedure and characterized the immunological response directed against the viral vector in this model. The present study reports on beta-gal expression for at least 7 months in neuronal and glial cells. Maturation of the transplanted fetal SCN with respect to immunoreactivity for vasoactive intestinal polypeptide and C-terminal propressophysin was not hampered by the viral infection.
van Esseveldt
K E
KE
Graduate School Neurosciences Amsterdam, Netherlands Institute for Brain Research. I.van.esseveldt@nih.knaw.nl
Hermens
W T
WT
Verhaagen
J
J
Boer
G J
GJ
D000256:Adenoviridae / Q000235:genetics*; D000818:Animals; D016380:Brain Tissue Transplantation / Q000276:immunology; D002478:Cells, Cultured; D016332:Fetal Tissue Transplantation; D005786:Gene Expression Regulation; D018014:Gene Transfer Techniques; D005822:Genetic Vectors; D007031:Hypothalamus / Q000637:transplantation; D017403:In Situ Hybridization; D009924:Organ Culture Techniques; D051381:Rats; D011919:Rats, Inbred Strains; D017208:Rats, Wistar; D013493:Suprachiasmatic Nucleus / Q000637:transplantation; D013997:Time Factors; D019076:Transgenes