title: Putative protein serine/threonine kinase genes are located in several positions on the chromosome of Streptomyces coelicolor A3(2).
abstract: Seven DNA fragments containing putative protein serine/threonine kinase genes were cloned from Streptomyces coelicolor A3(2) by hybridization with a 165-bp amplified polymerase chain reaction product of pkaB containing kinase subdomains VI, VII and VIII. Among them, the nucleotide sequences of three fragments containing subdomains VI, VII and VIII were identical, while those of one fragment were identical with that of afsK, another protein serine/threonine kinase gene. However, these kinase-like genes with very similar nucleotide sequences were distributed on the chromosome of S. coelicolor A3(2).
authors.affiliation: Department of Biochemistry, Meiji College of Pharmacy, Tokyo, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Hirakata
authors.forename: H
authors.initials: H
authors.lastname: Kieser
authors.forename: D
authors.initials: D
authors.lastname: Hopwood
authors.forename: H
authors.initials: H
authors.lastname: Urabe
authors.forename: H
authors.initials: H
authors.lastname: Ogarawa
mesh_terms: D001483:Base Sequence; D002874:Chromosome Mapping; D003001:Cloning, Molecular; D004269:DNA, Bacterial / Q000032:analysis; D008969:Molecular Sequence Data; D017346:Protein Serine-Threonine Kinases / Q000235:genetics*; D017422:Sequence Analysis, DNA; D013302:Streptomyces / Q000201:enzymology / Q000235:genetics*
chemical_list: D004269:DNA, Bacterial; D017346:Protein Serine-Threonine Kinases