title: A conserved disulfide motif in human tear lipocalins influences ligand binding.
abstract: Structural and functional characteristics of the disulfide motif have been determined for tear lipocalins, members of a novel group of proteins that carry lipids. Amino acid sequences for two of the six isolated isoforms were assigned by a comparison of molecular mass measurements with masses calculated from the cDNA-predicted protein sequence and available N-terminal protein sequence data. A third isoform was tentatively sequence assigned using the same criteria. The most abundant isoform has a measured mass of 17 446.3 Da, consistent with residues 19-176 of the putative precursor (calculated mass 17 445.8 Da). Chemical derivatization of native and reduced/denatured protein confirmed the presence of a single intramolecular disulfide bond in the native protein. Reactivity of native, reduced, and denatured protein with 4-pyridine disulfide and dithiobis(2-nitrobenzoic acid) indicated that access to the free cysteine is markedly restricted by the intact disulfide bridge. Mass measurements of tryptic fragments identified C119 as the free cysteine and showed that the single intramolecular disulfide bond joined residues C79 and C171. Circular dichroism indicated that tear lipocalins have a predominant beta-pleated sheet structure (44%) that is essentially retained after reduction of the disulfide bond. Circular dichroism in the far-UV showed reduced molecular asymmetry and enhanced urea-induced unfolding with disulfide reduction indicative of relaxation of protein structure. Circular dichroism in the near-UV shows that the disulfide bond contributes to the asymmetry of aromatic sites. The effect of disulfide reduction on ligand binding was monitored using the intrinsic optical activity of bound retinol. The intact disulfide bond diminishes the affinity of tear lipocalins for retinol and restricts the displacement of native lipids by retinol. Disulfide reduction is accompanied by a dramatic alteration in ligand-induced conformational changes that involves aromatic residues. The disulfide bridge in tear lipocalins is important in conferring protein rigidity and influencing ligand affinity. The disulfide bond appears highly conserved so that these findings may have implications for the entire lipocalin superfamily.
authors.affiliation: Department of Pathology, UCLA School of Medicine, Los Angeles, California 90095, USA.
authors.forename: B J
authors.initials: BJ
authors.lastname: Glasgow
authors.forename: A R
authors.initials: AR
authors.lastname: Abduragimov
authors.forename: T N
authors.initials: TN
authors.lastname: Yusifov
authors.forename: O K
authors.initials: OK
authors.lastname: Gasymov
authors.forename: J
authors.initials: J
authors.lastname: Horwitz
authors.forename: W L
authors.initials: WL
authors.lastname: Hubbell
authors.forename: K F
authors.initials: KF
authors.lastname: Faull
mesh_terms: D000595:Amino Acid Sequence; D001665:Binding Sites; D002352:Carrier Proteins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D002942:Circular Dichroism; D017124:Conserved Sequence; D004220:Disulfides / Q000737:chemistry; D006801:Humans; D066298:In Vitro Techniques; D008024:Ligands; D054835:Lipocalin 1; D013058:Mass Spectrometry; D008969:Molecular Sequence Data; D010446:Peptide Fragments / Q000737:chemistry / Q000302:isolation & purification; D011487:Protein Conformation; D017433:Protein Structure, Secondary; D013666:Tears / Q000737:chemistry*; D014357:Trypsin
chemical_list: D002352:Carrier Proteins; D004220:Disulfides; C518443:LCN1 protein, human; D008024:Ligands; D054835:Lipocalin 1; D010446:Peptide Fragments; D014357:Trypsin