title: Calcium binding to polypeptides of rat liver and Zajdela hepatoma mitochondrial inner membranes.
abstract: Composition and amount of 45Ca2+-binding proteins in the inner membrane fraction of rat liver and Zajdela hepatoma mitochondria were determined. In the inner membrane of liver mitochondria, three major 45Ca2+-binding polypeptides: a protein of approximately 130 kDa (carbamoyl-phosphate synthetase), a glycoprotein of 43-44 kDa (previously considered as the calcium uniporter), and 29-30 kDa protein were found. These components were absent (130 kDa component) or relatively reduced (43-44 kDa and 29-30 kDa components) in the inner membrane of hepatoma mitochondria. Previously unknown low molecular mass polypeptides, having very high Ca2+-binding ability, were found in the inner membrane of hepatoma mitochondria. One of them might be the natural Ca2+-binding inhibitor of H+-ATPase.
authors.affiliation: Institute of Theoretical and Experimental Biophysics of the Russian Academy of Sciences, Pushchino, Moscow Region. evtodienko@venus.iteb.serpukhov.su
authors.forename: Y V
authors.initials: YV
authors.lastname: Evtodienko
authors.forename: T S
authors.initials: TS
authors.lastname: Azarashvili
authors.forename: A P
authors.initials: AP
authors.lastname: Kudin
mesh_terms: D000818:Animals; D002135:Calcium-Binding Proteins / Q000032:analysis*; D006528:Carcinoma, Hepatocellular; D002478:Cells, Cultured; D007425:Intracellular Membranes / Q000737:chemistry*; D008099:Liver / Q000737:chemistry* / Q000166:cytology; D008297:Male; D008565:Membrane Proteins / Q000032:analysis*; D008928:Mitochondria / Q000737:chemistry*; D010455:Peptides / Q000032:analysis*; D051381:Rats; D017208:Rats, Wistar; D014407:Tumor Cells, Cultured
chemical_list: D002135:Calcium-Binding Proteins; D008565:Membrane Proteins; D010455:Peptides