title: Chicken keratin-19: cloning of cDNA and analysis of expression in the chicken embryonic gut.
abstract: From many recent studies, it has been argued that keratins (cytokeratins) play important roles in the morphogenesis and differentiation of organ development. To learn the role of keratin in digestive tract development, a cDNA of the chicken homolog of keratin-19 (GK-19) was cloned and its expression pattern was analyzed in the digestive tract of chicken embryos. The GK-19 full-length sequence was approximately 1.6 kb and showed more than 80% similarity to human and mouse keratin-19. The result of in situ hybridization with the proventriculus (glandular stomach) of different developmental stages showed that GK-19 expression disappeared specifically in the glandular epithelium from day 6 to day 9 of incubation. Furthermore, GK-19 was localized in the notochord, floor plate, anterior lobe of the pituitary gland and mesonephros. These results suggest the possibility that GK-19 may have multiple roles in organogenesis during embryogenesis.
authors.affiliation: Department of Biology, Faculty of Science, Tokyo Metropolitan University, Hachioji, Japan.
authors.forename: K
authors.initials: K
authors.lastname: Sato
authors.forename: S
authors.initials: S
authors.lastname: Yasugi
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002454:Cell Differentiation / Q000502:physiology; D002642:Chick Embryo; D003001:Cloning, Molecular; D004064:Digestive System / Q000166:cytology / Q000196:embryology*; D018507:Gene Expression Regulation, Developmental / Q000235:genetics*; D017403:In Situ Hybridization; D007633:Keratins / Q000737:chemistry* / Q000502:physiology; D008969:Molecular Sequence Data; D009672:Notochord / Q000166:cytology / Q000254:growth & development; D012333:RNA, Messenger / Q000032:analysis; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid
chemical_list: D012333:RNA, Messenger; D007633:Keratins