title: Identification and characterization of a novel trans-membrane protein gene, pdh1, from Schizosaccharomyces pombe.
abstract: We have cloned a new gene, pdh1, from genomic DNA of fission yeast Schizosaccharomyces pombe. pdh1 is actively transcribed as 1400-nucleotide mRNA in vegetatively growing cells and can code for a 226 amino acid polypeptide (pdh1p). Computational structural prediction has revealed that the pdh1p is a highly hydrophobic protein with seven transmembrane domains. The prediction has also detected a possible C-kinase phosphorylation site within the longest hydrophilic loop.
authors.affiliation: National Institute of Genetics, Shizuoka, Japan.
authors.forename: H
authors.initials: H
authors.lastname: Iha
authors.forename: M
authors.initials: M
authors.lastname: Takimoto
authors.forename: I
authors.initials: I
authors.lastname: Danjoh
authors.forename: A
authors.initials: A
authors.lastname: Fujiyama
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D001665:Binding Sites; D002462:Cell Membrane / Q000378:metabolism; D018744:DNA, Plant; D015247:Deoxyribonuclease HindIII / Q000378:metabolism; D015252:Deoxyribonucleases, Type II Site-Specific / Q000378:metabolism; D005656:Fungal Proteins / Q000235:genetics*; D008565:Membrane Proteins / Q000235:genetics* / Q000378:metabolism; D008969:Molecular Sequence Data; D012568:Schizosaccharomyces / Q000235:genetics* / Q000254:growth & development; D029702:Schizosaccharomyces pombe Proteins
chemical_list: D018744:DNA, Plant; D005656:Fungal Proteins; D008565:Membrane Proteins; D029702:Schizosaccharomyces pombe Proteins; C491756:pdh1 protein, S pombe; D015247:Deoxyribonuclease HindIII; D015252:Deoxyribonucleases, Type II Site-Specific; C029312:GGTACC-specific type II deoxyribonucleases