Oxidative stress-related parameters in prostate of rats with experimental autoimmune prostatitis.
BACKGROUND
Oxidative stress in tissues can be provoked by an augmented metabolic rate, which may sometimes be combined with a decrease in the antioxidant capacity.


METHODS
In this study we examined the primary enzymatic defense mechanisms against the damage caused by reactive oxygen species (ROS): the superoxide dismutase (SOD) and catalase activities and glutathione content, as well as the levels of total thiobarbituric acid-reactant substances (TBARS), indicative of lipid peroxidation. These studies were made in prostate homogenates of rats with experimental autoimmune prostatitis (EAP) and of control rats treated with complete Freund's adjuvant (CFA) or nontreated.


RESULTS
The evaluation of antioxidant defenses revealed a significant diminution of the catalase activity in autoimmune rats without changes in SOD activity and glutathione content. TBARS levels evidenced a significant increase in prostate homogenates from autoimmune rats in relation to control rat samples.


CONCLUSIONS
The results suggest that in EAP, a marked diminution of catalase activity associated with an enhanced oxidative metabolism of inflammatory macrophages might lead to oxidative damage in this autoimmune disease.
Orsilles
M A
MA
Departamento de Bioquímica Clínica, Facultad de Ciencias Químicas, Universidad Nacional de Córdoba, Argentina. mirtha@bioclin.uncor.edu
Depiante-Depaoli
M
M
D000818:Animals; D001327:Autoimmune Diseases / Q000139:chemically induced / Q000378:metabolism* / Q000473:pathology; D002374:Catalase / Q000378:metabolism*; D004195:Disease Models, Animal; D005978:Glutathione / Q000378:metabolism*; D015227:Lipid Peroxidation; D008297:Male; D018384:Oxidative Stress; D011467:Prostate / Q000378:metabolism* / Q000473:pathology; D011472:Prostatitis / Q000139:chemically induced / Q000378:metabolism* / Q000473:pathology; D051381:Rats; D017208:Rats, Wistar; D013482:Superoxide Dismutase / Q000378:metabolism*
D002374:Catalase; D013482:Superoxide Dismutase; D005978:Glutathione