title: Effects of short-term feeding of rats with a highly purified phaseolin preparation.
abstract: Rats fed a diet containing a highly purified preparation of phaseolin, the main globulin from the seeds of Phaseolus vulgaris, rapidly lost weight. Fecal nitrogen outputs were elevated and the N digestibility, based on conventional method estimation, was only 37.5%. By using immunological techniques, however, it was shown that the bulk of the nitrogen recovered in the feces was not chemically related to phaseolin. After correction for this non-phaseolin N, the true digestibility of phaseolin was estimated to be 74.3%. It is suggested that phaseolin and/or undigested fragments derived from the native molecule may, as reported for the lectins, stimulate the secretion of endogenous N, possibly mucins.
authors.affiliation: The Rowett Research Institute, Aberdeen, Scotland. lsantoro@correionet.com.br
authors.forename: L G
authors.initials: LG
authors.lastname: Santoro
authors.forename: G
authors.initials: G
authors.lastname: Grant
authors.forename: A
authors.initials: A
authors.lastname: Pusztai
mesh_terms: D000818:Animals; D004044:Dietary Proteins / Q000008:administration & dosage*; D004063:Digestion; D004591:Electrophoresis, Polyacrylamide Gel; D007887:Fabaceae / Q000737:chemistry; D005243:Feces / Q000737:chemistry; D007421:Intestine, Small / Q000033:anatomy & histology; D007768:Lactalbumin / Q000008:administration & dosage; D009584:Nitrogen / Q000378:metabolism; D009929:Organ Size; D010940:Plant Proteins; D010946:Plants, Medicinal; D051381:Rats; D012639:Seeds / Q000737:chemistry; D015431:Weight Loss
chemical_list: D004044:Dietary Proteins; D010940:Plant Proteins; C048826:phaseolin protein, Phaseolus vulgaris; D007768:Lactalbumin; D009584:Nitrogen