[The release and absorption of small ribonucleoprotein complexes (alpha RNPs) in cultures of transformed rat fibroblasts and human epidermoid carcinoma].
The release of alpha RNPs and their absorbtion by the cells from culture medium were studied. The rat fibroblasts of parental serum-dependent cell line LRec-1, and of selected serum-free cell line LRec-1sf rapidly released and absorbed alpha RNPs. In the latter case, both auto- and heterologous alpha RNPs were seen to penetrate, whereas only autologous alpha RNPs entered LRec-1 cells. Besides, the ability to rapid export and absorbtion of autocrine alpha RNPs was demonstrated for human epidermoid carcinoma cell line A431. Both LRec-1 and LRec-1sf cells expressed mRNAs with homology to ID-like nucleotide sequences, the level of mRNA expression decreasing markedly when serum-dependent LRec-1 cells were grown in serum-free medium.
Pleskach
V A
VA
Institute of Cytology, Russian Academy of Sciences, St. Petersburg.
Il'kaeva
O R
OR
Kozhukharova
I V
IV
Artsybasheva
I V
IV
Turoverova
L V
LV
Konstaninova
I M
IM
D000818:Animals; D002294:Carcinoma, Squamous Cell / Q000378:metabolism*; D002461:Cell Line, Transformed / Q000378:metabolism*; D002478:Cells, Cultured; D003470:Culture Media; D016895:Culture Media, Serum-Free; D004622:Embryo, Mammalian; D005347:Fibroblasts / Q000378:metabolism; D006801:Humans; D051381:Rats; D017411:Ribonucleoproteins, Small Nuclear / Q000032:analysis / Q000378:metabolism*; D014316:Tritium; D014407:Tumor Cells, Cultured; D014529:Uridine / Q000378:metabolism
D003470:Culture Media; D016895:Culture Media, Serum-Free; D017411:Ribonucleoproteins, Small Nuclear; D014316:Tritium; D014529:Uridine
Eksport i pogloshchenie malykh ribonukleoproteinovykh kompleksov (al'fa RNP) v kul'turakh transformirovannykh krysinykh fibroblastov i épidermoidnoĭ kartsinomy cheloveka.