Measurement of whole blood phagocyte chemiluminescence in the Wistar rat.
The factors influencing the rat whole blood chemiluminescence (CL): concentrations of blood, luminol, zymosan or opsonized zymosan, volume of the reaction mixture, storage time of blood samples and the presence of anticoagulants were evaluated. The CL micromethod described provides a fast and sensitive tool for the determination of metabolic activity of phagocytes in the microlitre range of rat whole blood.
Lojek
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A
Institute of Biophysics, Academy of Sciences of the Czech Republic. Brno, Czech Republic.
Ciz
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M
Marnila
P
P
Duskova
M
M
Lilius
E M
EM
D000818:Animals; D000925:Anticoagulants / Q000494:pharmacology; D001793:Blood Preservation; D001800:Blood Specimen Collection; D001810:Blood Volume; D007202:Indicators and Reagents; D007700:Kinetics; D008163:Luminescent Measurements; D008165:Luminol; D008297:Male; D010586:Phagocytes / Q000187:drug effects / Q000502:physiology*; D051381:Rats; D017208:Rats, Wistar; D012680:Sensitivity and Specificity; D015054:Zymosan
D000925:Anticoagulants; D007202:Indicators and Reagents; D008165:Luminol; D015054:Zymosan