Conservation of T cell receptor conformation in epidermal gammadelta cells with disrupted primary Vgamma gene usage.
A feature that distinguishes gammadelta T cell subsets from most alphabeta T cells and B cells is the association of expression of single T cell receptor (TCR) gamma and delta variable (V) region gene segments with specific anatomic sites. Mice lacking the TCR Vgamma5 chain normally expressed by most dendritic epidermal T cells were shown to retain a conformational determinant (idiotype) ordinarily expressed exclusively by such Vgamma5+ cells. Conservation by shuffled gammadelta TCR chains of an idiotype associated with a specific anatomic site indicates that for TCRgammadelta, as for immunoglobulin, conformation is associated to a greater extent with the function or development of lymphocyte repertoires than is the use of particular gene segments.
Mallick-Wood
C A
CA
Department of Molecular, Cell, and Developmental Biology and Section of Immunobiology, Yale University, New Haven, CT 06520, USA.
Lewis
J M
JM
Richie
L I
LI
Owen
M J
MJ
Tigelaar
R E
RE
Hayday
A C
AC
D000818:Animals; D002460:Cell Line; D003713:Dendritic Cells / Q000276:immunology*; D000078404:Epidermal Cells; D004817:Epidermis / Q000276:immunology*; D000939:Epitopes / Q000032:analysis; D005260:Female; D015321:Gene Rearrangement; D006825:Hybridomas; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length; D011487:Protein Conformation; D016692:Receptors, Antigen, T-Cell, gamma-delta / Q000737:chemistry / Q000235:genetics* / Q000276:immunology*; D016176:T-Lymphocyte Subsets / Q000276:immunology*
D000939:Epitopes; D016692:Receptors, Antigen, T-Cell, gamma-delta