title: The effect of diazepan and exercise training on selected biochemical and histochemical properties of rat skeletal muscle.
abstract: The effects of chronic diazepam (D) treatment and exercise training on total body mass (TBM), microsomal protein yield (MPY), calcium uptake by fragmented sarcoplasmic reticulum (SR), muscle fibre cross-sectional area, and both PFK and SDH activities were investigated in the tibialis anterior (TA), soleus (Sol), and plantaris (Plt) muscles of 50 male albino Sprague-Dawley rats. Rats were assigned randomly to control (C), sprint-trained (S), or endurance-trained (E) groups. Training was of 12 weeks duration. One-half of each group received daily intraperitoneally D doses of 5 mg kg-1 of TBM. Exercise reduced TBM (p < 0.05); increased the relative BM of the TA (E = 2.02 +/- 0.02, p < 0.01) and Plt (E = 1.15 +/- 0.02, p < 0.01; S = 1.13 +/- 0.03, p < 0.01), as well as the Ca++ uptake of the Sol SR (C = 0.08 +/- 0.02, E = 0.16 +/- 01, p < 0.05). MPY was elevated in S-Sol (C = 1.12 +/- 0.6, S = 1.52 +/- 0.1, p < 0.01). D elevated Sol MPY as well as TA PFK. S-trained animals had lower mean fibre areas than the E-trained (D-treated and untreated) animals. The elevated relative masses of TA and Plt are explained by a decreased TBM with exercise. The increased Ca++ uptake of the Sol indicates that E enhances this function, and the increased MPY probably implies an increased SR. The D could be responsible for the D-elevated Sol MPY as well as the TA PFK. El D did not reduce neuromuscular activity to a level adversely affecting oxidative enzyme activity, but in the case of PFK activity in the TA muscle, such a reduction was evident.
authors.affiliation: Escuela Superior de Medicina, Instituto Politécnico Nacional CONACyT, México.
authors.forename: J
authors.initials: J
authors.lastname: Padilla
authors.forename: W C
authors.initials: WC
authors.lastname: Fielding
authors.forename: A N
authors.initials: AN
authors.lastname: Belcastro
authors.forename: P F
authors.initials: PF
authors.lastname: Gardiner
authors.forename: A W
authors.initials: AW
authors.lastname: Taylor
mesh_terms: D000704:Analysis of Variance; D000818:Animals; D001835:Body Weight; D002118:Calcium / Q000378:metabolism; D003975:Diazepam / Q000494:pharmacology*; D005631:Fructokinases / Q000378:metabolism; D008297:Male; D008861:Microsomes; D009125:Muscle Relaxants, Central / Q000494:pharmacology*; D018482:Muscle, Skeletal / Q000187:drug effects* / Q000378:metabolism; D010805:Physical Conditioning, Animal; D011506:Proteins; D051381:Rats; D017207:Rats, Sprague-Dawley; D013385:Succinate Dehydrogenase / Q000378:metabolism
chemical_list: D009125:Muscle Relaxants, Central; D011506:Proteins; D013385:Succinate Dehydrogenase; D005631:Fructokinases; D003975:Diazepam; D002118:Calcium