title: Iron chelation by chlorogenic acid as a natural antioxidant.
abstract: Chlorogenic acid, a dietary antioxidant, effectively inhibited the iron-induced lipid peroxidation of bovine liver microsomes in a concentration-dependent manner. In the Fenton-type reaction, chlorogenic acid inhibited the production of the hydroxyl radical by iron-EDTA or iron-ADP, while iron plus chlorogenic acid did not generate the hydroxyl radical. The formation of an iron complex with chlorogenic acid was demonstrated by UV/vis absorbance spectroscopic, ESR and 1H-NMR studies. The ferric complex with chlorogenic acid was in the ferric high-spin state near rhombicity, and had no radical scavenging activity. The results indicate that chlorogenic acid prevented the formation of the hydroxyl radical by forming a chelate with iron whose complex cannot catalyze the Fenton-type reaction.
authors.affiliation: Department of Life Science and Biotechnology, Faculty of Life and Environmental Science, Shimane University, Japan. ykono@life.shimane-u.ac.jp
authors.forename: Y
authors.initials: Y
authors.lastname: Kono
authors.forename: S
authors.initials: S
authors.lastname: Kashine
authors.forename: T
authors.initials: T
authors.lastname: Yoneyama
authors.forename: Y
authors.initials: Y
authors.lastname: Sakamoto
authors.forename: Y
authors.initials: Y
authors.lastname: Matsui
authors.forename: H
authors.initials: H
authors.lastname: Shibata
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000378:metabolism*; D002417:Cattle; D002614:Chelating Agents / Q000378:metabolism*; D002726:Chlorogenic Acid / Q000378:metabolism*; D017665:Hydroxyl Radical / Q000378:metabolism; D007501:Iron / Q000378:metabolism / Q000494:pharmacology*; D015227:Lipid Peroxidation; D015394:Molecular Structure
chemical_list: D000975:Antioxidants; D002614:Chelating Agents; D002726:Chlorogenic Acid; D017665:Hydroxyl Radical; D007501:Iron