Multicenter evaluation of the Paragon CZE 2000 capillary zone electrophoresis system for serum protein electrophoresis and monoclonal component typing.
Serum protein electrophoresis and typing of monoclonal components (MCs) are routine but time-consuming and technically demanding assays. We evaluated capillary electrophoresis (Paragon CZE 2000) for automation of the two assays. CZE and cellulose acetate electrophoresis gave similar data on 794 samples. Within-run and between-run CVs were < 2% for albumin and gamma-globulins and 4-7% for alpha 1-, alpha 2-, and beta-globulins. Bilirubin, hemoglobin, triglycerides, and fibrinogen were found not to interfere. No carryover by capillaries was detected. The detection limit for MC was < 0.5 g/L. MC assessment by immunosubtraction on 403 samples identified the monoclonal type in all samples with peak concentrations > 10 g/L; only 50% of MCs that could not be quantified by densitometric scan were typed.
Bienvenu
J
J
Laboratoire d'Immunologie, Centre Hospitalier Lyon Sud, Pierre-Bénite, France.
Graziani
M S
MS
Arpin
F
F
Bernon
H
H
Blessum
C
C
Marchetti
C
C
Righetti
G
G
Somenzini
M
M
Verga
G
G
Aguzzi
F
F
D000911:Antibodies, Monoclonal; D001663:Bilirubin / Q000097:blood; D001798:Blood Proteins / Q000302:isolation & purification*; D019075:Electrophoresis, Capillary / Q000295:instrumentation* / Q000379:methods; D004588:Electrophoresis, Cellulose Acetate / Q000379:methods; D005340:Fibrinogen; D006454:Hemoglobins; D006801:Humans; D007122:Immunoelectrophoresis / Q000379:methods; D007753:Laboratories / Q000592:standards; D009391:Nephelometry and Turbidimetry / Q000379:methods; D009404:Nephrotic Syndrome / Q000097:blood; D011786:Quality Control; D012016:Reference Values; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D012709:Serum Albumin / Q000302:isolation & purification; D012712:Serum Globulins / Q000302:isolation & purification; D014280:Triglycerides / Q000097:blood; D005719:gamma-Globulins / Q000302:isolation & purification
D000911:Antibodies, Monoclonal; D001798:Blood Proteins; D006454:Hemoglobins; D012709:Serum Albumin; D012712:Serum Globulins; D014280:Triglycerides; D005719:gamma-Globulins; D005340:Fibrinogen; D001663:Bilirubin