The effects of treatment with chemical agents or infection with feline viruses on protein-binding properties of the feline immunodeficiency virus long terminal repeat.
The effects of treatment with chemical agents or infection with feline viruses on protein-binding properties of the feline immunodeficiency virus (FIV) long terminal repeat (LTR) were examined by gel-mobility-shift assays using oligonucleotides designed to represent putative AP-1 or ATF motif from the FIV LTR. Infection with FIV led to less nuclear proteins binding to the AP-1 and ATF sites, suggesting that proteins binding to the sites were consumed or suppressed by FIV-replication in FIV-infected cells. Nuclear proteins that bind to the AP-1 or ATF site were examined by using extracts from Crandell feline kidney (CRFK) cells treated with TPA (a phorbol ester; a strong activator of protein kinase C) or forskolin (an inducer of cyclic-AMP), or infection with feline herpesvirus type 1 (FHV-1). Although TPA or forskolin treatment moderately increased the level of both proteins that bound to AP-1 and ATF sites, FHV-1 infection markedly changed the protein-binding patterns of the sites. Furthermore, FHV-1-induced proteins that bind adjacent to the transcriptional initiation site of FIV promoter were also observed in FHV-1-infected CRFK cells, suggesting that the FHV-1-induced-proteins affects the transcription of FIV through the AP-1, ATF and leader sequences.
Ikeda
Y
Y
Department of Veterinary Microbiology, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Japan.
Kawaguchi
Y
Y
Inoshima
Y
Y
Kohmoto
M
M
Shimojima
M
M
Inada
G
G
Sato
E
E
Kai
C
C
Miyazawa
T
T
Mikami
T
T
D000818:Animals; D001665:Binding Sites; D002415:Cats; D002460:Cell Line; D005576:Colforsin / Q000494:pharmacology*; D006564:Herpesviridae / Q000502:physiology*; D016089:Immunodeficiency Virus, Feline / Q000235:genetics*; D009687:Nuclear Proteins / Q000187:drug effects / Q000378:metabolism*; D010442:Peptide Chain Initiation, Translational; D012091:Repetitive Sequences, Nucleic Acid; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology*; D014158:Transcription, Genetic
D009687:Nuclear Proteins; D005576:Colforsin; D013755:Tetradecanoylphorbol Acetate