title: Molecular cloning, genomic characterization and expression of novel human alpha1A-adrenoceptor isoforms.
abstract: We have isolated and characterized from human prostate novel splice variants of the human alpha1A-adrenoceptor, several of which generate truncated products and one isoform, alpha(1A-4), which has the identical splice site as the three previously described isoforms. Long-PCR on human genomic DNA showed that the alpha(1A-4) exon is located between those encoding the alpha(1A-1) and alpha(1A-3) variants. CHO-K1 cells stably expressing alpha(1A-4) showed ligand binding properties similar to those of the other functional isoforms as well as agonist-stimulated inositol phosphate accumulation. Quantitative PCR analyses revealed that alpha(1A-4) is the most abundant isoform expressed in the prostate with high levels also detected in liver and heart.
authors.affiliation: Center for Biological Research, Neurobiology Unit, Roche Bioscience, Palo Alto, CA 94304, USA. david-j.chang@roche.com
authors.forename: D J
authors.initials: DJ
authors.lastname: Chang
authors.forename: T K
authors.initials: TK
authors.lastname: Chang
authors.forename: S S
authors.initials: SS
authors.lastname: Yamanishi
authors.forename: F H
authors.initials: FH
authors.lastname: Salazar
authors.forename: A H
authors.initials: AH
authors.lastname: Kosaka
authors.forename: R
authors.initials: R
authors.lastname: Khare
authors.forename: S
authors.initials: S
authors.lastname: Bhakta
authors.forename: J R
authors.initials: JR
authors.lastname: Jasper
authors.forename: I S
authors.initials: IS
authors.lastname: Shieh
authors.forename: J D
authors.initials: JD
authors.lastname: Lesnick
authors.forename: A P
authors.initials: AP
authors.lastname: Ford
authors.forename: D V
authors.initials: DV
authors.lastname: Daniels
authors.forename: R M
authors.initials: RM
authors.lastname: Eglen
authors.forename: D E
authors.initials: DE
authors.lastname: Clarke
authors.forename: C
authors.initials: C
authors.lastname: Bach
authors.forename: H W
authors.initials: HW
authors.lastname: Chan
mesh_terms: D000317:Adrenergic alpha-Antagonists / Q000494:pharmacology*; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D016466:CHO Cells; D003001:Cloning, Molecular; D006224:Cricetinae; D015723:Gene Library; D006801:Humans; D007295:Inositol Phosphates / Q000378:metabolism*; D008297:Male; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D011467:Prostate / Q000378:metabolism*; D012333:RNA, Messenger / Q000378:metabolism; D011869:Radioligand Assay; D018340:Receptors, Adrenergic, alpha-1 / Q000096:biosynthesis / Q000737:chemistry / Q000502:physiology*; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000737:chemistry; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid; D014162:Transfection
chemical_list: C547713:ADRA1A protein, human; D000317:Adrenergic alpha-Antagonists; D007295:Inositol Phosphates; D012333:RNA, Messenger; D018340:Receptors, Adrenergic, alpha-1; D011994:Recombinant Proteins