title: H2-uptake activity, spectra, reduction potentials, and kinetics of iron release on the surface of iron core from Azotobacter vinelandii bacterial ferritin.
abstract: Bacterial ferritin from Azotobacter vinelandii (AvBFo) has a function in H2 uptake. The Fe3+ reduction on the surface of the iron core from AvBFo is accompanied simultaneously by H2 uptake, with a maximum activity of H2 uptake of 450 H2/AvBFo. A reduction potential of -402 mV for iron reduction on the surface of the core is found. A shift to the red the protein absorbance peaks ranging from 280 to 290 nm is observed between pH 5 and 9 under 100% H2 reduction. The reduction potential for iron release becomes negative at a rate of 0.025 mV/Fe2+ released. The kinetics of iron release on the surface of the core is a first-order reaction.
authors.affiliation: Department of Biology, Xianen University, China.
authors.forename: H Q
authors.initials: HQ
authors.lastname: Huang
authors.forename: L S
authors.initials: LS
authors.lastname: Xu
authors.forename: F Z
authors.initials: FZ
authors.lastname: Zhang
authors.forename: X H
authors.initials: XH
authors.lastname: Qui
authors.forename: Q M
authors.initials: QM
authors.lastname: Lin
authors.forename: J W
authors.initials: JW
authors.lastname: Huang
authors.forename: H
authors.initials: H
authors.lastname: Zao
authors.forename: N C
authors.initials: NC
authors.lastname: Huang
authors.forename: R Y
authors.initials: RY
authors.lastname: Zeng
authors.forename: D
authors.initials: D
authors.lastname: Zeng
mesh_terms: D016948:Azotobacter vinelandii / Q000737:chemistry* / Q000378:metabolism; D001426:Bacterial Proteins / Q000737:chemistry* / Q000378:metabolism; D005293:Ferritins / Q000737:chemistry* / Q000378:metabolism; D006859:Hydrogen / Q000737:chemistry* / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D010084:Oxidation-Reduction; D013056:Spectrophotometry, Ultraviolet
chemical_list: D001426:Bacterial Proteins; D006859:Hydrogen; D005293:Ferritins