title: Genomic discordance between monozygotic twins discordant for schizophrenia.
abstract: OBJECTIVE
  Genomic DNA of monozygotic twins discordant for schizophrenia was analyzed to determine whether their genomes were truly identical.
  
  
  METHODS
  The subjects were monozygotic male twins, one of whom had DSM-III-R schizophrenia, undifferentiated type. Genomic DNA was extracted from leukocytes and was applied to restriction landmark genome scanning analysis, which was developed for a high-speed survey of restriction sites throughout a genome and measurement of their copy number in each locus.
  
  
  RESULTS
  After comparisons of patterns with approximately 2,000 spots, the authors detected at least two spots with autoradiographic intensities that obviously differed in the two twins.
  
  
  CONCLUSIONS
  The discrepancies likely were generated either by differences in the methylation status at NotI sites between the twins or by submicroscopic changes occurring at NotI-flanking sites in one twin after (or simultaneous with) twinning. In either case, the difference may influence the transcription level of one or more genes.
authors.affiliation: Department of Human Genetics, Nagasaki University School of Medicine, Japan. f0932@cc.nagasaki-u.ac.jp
authors.forename: T
authors.initials: T
authors.lastname: Tsujita
authors.forename: N
authors.initials: N
authors.lastname: Niikawa
authors.forename: H
authors.initials: H
authors.lastname: Yamashita
authors.forename: A
authors.initials: A
authors.lastname: Imamura
authors.forename: A
authors.initials: A
authors.lastname: Hamada
authors.forename: Y
authors.initials: Y
authors.lastname: Nakane
authors.forename: Y
authors.initials: Y
authors.lastname: Okazaki
mesh_terms: D000328:Adult; D001345:Autoradiography; D004247:DNA / Q000032:analysis / Q000235:genetics*; D004200:Diseases in Twins / Q000235:genetics*; D008040:Genetic Linkage; D006801:Humans; D008297:Male; D015183:Restriction Mapping; D012559:Schizophrenia / Q000235:genetics*; D014430:Twins, Monozygotic
chemical_list: D004247:DNA