Genomic structure and chromosomal mapping of the mouse STOP gene (Mtap6).
The microtubule associated protein STOP (Stable Tubule Only Polypeptide) is a calmodulin-regulated protein able to induce a high degree of microtubule stability. STOP is abundant in neurons which contain large subpopulations of stable microtubules. Genomic clones spanning 67 kb and encompassing the mouse STOP gene (Mtap6) have been isolated and characterized. These clones derive from a single gene mapping to the E2-F1 region of mouse chromosome 7. The gene is composed of 4 exons that exhibit conventional vertebrate splicing sequences. Transcription of the gene initiate at multiple sites in a 85 nucleotide region located 530 bases upstream the translation initiation codon. Accordingly, the 5' flanking region of the gene lacks a TATA box or an initiator element at usual position. The protein encoded by the mouse STOP gene (Mtap6) is composed of 906 amino acids and presents a 91% identities with the rat brain STOP.
Denarier
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CEA Laboratoire du Cytosquelette, INSERM Unité 366, Département de Biologie Moléculaire et Structurale, Commissariat à l'Energie Atomique de Grenoble, France.
Aguezzoul
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Jolly
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Vourc'h
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Roure
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Andrieux
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Bosc
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Job
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D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002874:Chromosome Mapping; D004247:DNA / Q000737:chemistry*; D005091:Exons; D051379:Mice; D008869:Microtubule-Associated Proteins / Q000737:chemistry / Q000235:genetics*; D008969:Molecular Sequence Data; D012326:RNA Splicing; D051381:Rats; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D016385:TATA Box; D014158:Transcription, Genetic
C113727:Map6 protein, rat; D008869:Microtubule-Associated Proteins; C491893:Mtap6 protein, mouse; D004247:DNA