A high-affinity folate binding protein in fluid of benign cysts of human liver and mammary gland.
The presence of a folate binding protein in fluid of benign cysts of human liver and mammary gland was demonstrated. Radioligand binding was of a high-affinity type (K approximately 10(10) M -1). The gel filtration profile of cystic fluid contained two peaks of radiolabelled folate, a large one of 25 kDa and a small one of 100 kDa. The concentration of radioligand bound protein in samples of cystic fluid ranged from nil to 6 nM. In most cases the protein immunoreacted with antibodies against human milk folate binding protein. The data suggest that fluid of human liver and mammary gland cysts contains a folate binding protein which appears to be homologous to human milk folate binding protein.
Holm
J
J
Department of Clinical Chemistry, Horsens Hospital.
Hansen
S I
SI
Høier-Madsen
M
M
D001667:Binding, Competitive; D002352:Carrier Proteins / Q000032:analysis* / Q000737:chemistry / Q000378:metabolism; D002850:Chromatography, Gel; D003560:Cysts / Q000737:chemistry*; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D005348:Fibrocystic Breast Disease / Q000737:chemistry*; D058973:Folate Receptors, GPI-Anchored; D005492:Folic Acid / Q000032:analysis / Q000378:metabolism*; D006801:Humans; D008107:Liver Diseases / Q000378:metabolism*; D008970:Molecular Weight; D011869:Radioligand Assay; D011956:Receptors, Cell Surface; D014316:Tritium
D002352:Carrier Proteins; D058973:Folate Receptors, GPI-Anchored; D011956:Receptors, Cell Surface; D014316:Tritium; D005492:Folic Acid