[Urease-negative Helicobacter pylori isolates from gastrointestinal mucosa of patients with peptic ulcer].
It is well known that Helicobacter pylori produced a large amount of urease which plays an important role in the maintenance of infection and adhesion. Two thousand three hundred and thirty-one specimens of the gastrointestinal mucosa were obtained from patients with peptic ulcer to isolate H. pylori strains. Of these specimens, 1602 strains of H. pylori were isolated and 7 urease-negative H. pylori strains were found. Biological characteristics (except urease production) of urease-negative H. pylori were in accord with urease-positive reference strains (ATCC strains). Furthermore, these urease-negative strains were confirmed to be H. pylori strains by PCR. These results suggest that the emergence of urease-negative strains pose a new problem for H. pylori infection in patients with peptic ulcer.
Muraoka
H
H
Chemotherapy Division, Mitsubishi-Kagaku Bio-Clinical Laboratories.
Kobayashi
I
I
Hasegawa
M
M
Saika
T
T
Toda
H
H
Nishida
M
M
Suzuki
J
J
Mine
T
T
Fujita
T
T
D005753:Gastric Mucosa / Q000382:microbiology*; D016480:Helicobacter pylori / Q000201:enzymology / Q000302:isolation & purification*; D006801:Humans; D007413:Intestinal Mucosa / Q000382:microbiology*; D010437:Peptic Ulcer / Q000382:microbiology*; D014510:Urease / Q000032:analysis*
D014510:Urease