title: Apoptosis-associated gene expression in the corpus luteum of the rat.
abstract: The involution of the corpus luteum (CL) at parturition is an example of physiological apoptosis, a complex process involving massive vascular regression while luteal cells undergo apoptosis. In the present study, changes in gene expression associated with physiological apoptosis were examined. Three genes isolated in our laboratory because of their association with apoptotic processes in the ovary, mammary gland, and prostate served as the focus of our investigation: Y81, Gas-1, and the gene IAP encoding integrin-associated protein. Y81 is a novel gene for which three transcripts are apparent. A Y81 cDNA clone representing the longest transcript has been isolated; it shows an open reading frame exhibiting a region of very high homology with members of the frizzled family, the prototypes of which are cell autonomous polarity genes encoding seven-pass transmembrane receptor proteins, for example the receptor for Wingless. Gas-1 is known as a growth-arrest gene that inhibits DNA synthesis when microinjected into cells. Integrin-associated protein is a beta 3-integrin-binding protein for which, recently, a thrombo-spondin-binding activity has been recognized. These three genes, all sharply up-regulated in the course of physiological involution processes in the ovarian CL, in mammary gland, and in prostate, seem promising candidates-by virtue of their specific expression in distinct tissues undergoing programmed cell death-as mediators of stimuli leading to apoptosis and subsequent phagocytosis. In this study, sulfated glycoprotein-2, previously observed in many instances of physiological apoptosis, was further employed as an indicator for incipient apoptosis, and stromelysin was followed as a marker for the tissue remodeling activity that is intimately associated with apoptosis during involution.
authors.affiliation: Department of Clinical Research, University of Berne, Switzerland.
authors.forename: K
authors.initials: K
authors.lastname: Guo
authors.forename: V
authors.initials: V
authors.lastname: Wolf
authors.forename: A M
authors.initials: AM
authors.lastname: Dharmarajan
authors.forename: Z
authors.initials: Z
authors.lastname: Feng
authors.forename: W
authors.initials: W
authors.lastname: Bielke
authors.forename: S
authors.initials: S
authors.lastname: Saurer
authors.forename: R
authors.initials: R
authors.lastname: Friis
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D017209:Apoptosis / Q000235:genetics*; D015415:Biomarkers; D018797:Cell Cycle Proteins; D051152:Clusterin; D003338:Corpus Luteum / Q000166:cytology* / Q000378:metabolism*; D005260:Female; D051157:Frizzled Receptors; D058851:GPI-Linked Proteins; D015870:Gene Expression; D006023:Glycoproteins / Q000235:genetics; D017403:In Situ Hybridization; D007743:Labor, Obstetric / Q000235:genetics; D003341:Luteolysis / Q000235:genetics; D008297:Male; D008321:Mammary Glands, Animal / Q000166:cytology / Q000378:metabolism; D019278:Matrix Metalloproteinase 3 / Q000235:genetics; D008565:Membrane Proteins / Q000235:genetics; D018832:Molecular Chaperones; D008969:Molecular Sequence Data; D049590:Postpartum Period / Q000235:genetics; D011247:Pregnancy; D011467:Prostate / Q000166:cytology / Q000378:metabolism; D011506:Proteins / Q000235:genetics; D051381:Rats; D017207:Rats, Sprague-Dawley; D017386:Sequence Homology, Amino Acid
chemical_list: D015415:Biomarkers; D018797:Cell Cycle Proteins; D051152:Clusterin; D051157:Frizzled Receptors; C096192:GAS1 protein, human; D058851:GPI-Linked Proteins; C501181:Gas1 protein, rat; D006023:Glycoproteins; D008565:Membrane Proteins; D018832:Molecular Chaperones; D011506:Proteins; D019278:Matrix Metalloproteinase 3