Sensitive method for the detection of fumonisin B1 in human urine.
Fumonisins are a group of carcinogenic mycotoxins that occur worldwide. This paper reports a sensitive method for the detection and quantitation of fumonisin B1 in human urine. Amberlite XAD-2 non-ionic polymeric adsorbent resin is used prior to strong anion-exchange (SAX) cartridge clean-up. As much as 100 ml of undiluted human urine can be loaded onto the column. Recoveries obtained using this method were 93.6, 95.1 and 94.4% when samples were spiked with 10, 50 and 500 ng/ml of fumonisin B1, respectively. This method is highly reproducible (R.S.D.<5%) and gives good sample clean-up, which is suitable for HPLC analysis.
Shetty
P H
PH
Food and Drug Toxicology Research Centre, National Institute of Nutrition, Indian Council of Medical Research, Hyderabad.
Bhat
R V
RV
D000327:Adsorption; D000837:Anion Exchange Resins; D002264:Carboxylic Acids / Q000652:urine*; D002274:Carcinogens, Environmental / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid; D037341:Fumonisins; D006801:Humans; D009183:Mycotoxins / Q000652:urine*; D011137:Polystyrenes; D015203:Reproducibility of Results; D012117:Resins, Synthetic; D012680:Sensitivity and Specificity
D000837:Anion Exchange Resins; D002264:Carboxylic Acids; D002274:Carcinogens, Environmental; D037341:Fumonisins; D009183:Mycotoxins; D011137:Polystyrenes; D012117:Resins, Synthetic; C056933:fumonisin B1; C003771:Amberlite XAD-2 resin