Update on Pneumocystis carinii f. sp. hominis typing based on nucleotide sequence variations in internal transcribed spacer regions of rRNA genes.
Pneumocystis carinii f. sp. hominis isolates from 207 clinical specimens from nine countries were typed based on nucleotide sequence variations in the internal transcribed spacer regions I and II (ITS1 and ITS2, respectively) of rRNA genes. The number of ITS1 nucleotides has been revised from the previously reported 157 bp to 161 bp. Likewise, the number of ITS2 nucleotides has been changed from 177 to 192 bp. The number of ITS1 sequence types has increased from 2 to 15, and that of ITS2 has increased from 3 to 14. The 15 ITS1 sequence types are designated types A through O, and the 14 ITS2 types are named types a through n. A total of 59 types of P. carinii f. sp. hominis were found in this study.
Lee
C H
CH
Department of Pathology and Laboratory Medicine, Indiana University School of Medicine, Indianapolis 46202, USA. chlee@iupui.edu
Helweg-Larsen
J
J
Tang
X
X
Jin
S
S
Li
B
B
Bartlett
M S
MS
Lu
J J
JJ
Lundgren
B
B
Lundgren
J D
JD
Olsson
M
M
Lucas
S B
SB
Roux
P
P
Cargnel
A
A
Atzori
C
C
Matos
O
O
Smith
J W
JW
D001483:Base Sequence; D004271:DNA, Fungal / Q000235:genetics; D004275:DNA, Ribosomal / Q000235:genetics; D008969:Molecular Sequence Data; D016533:Mycological Typing Techniques; D011010:Pneumocystis / Q000145:classification* / Q000235:genetics; D012331:RNA, Fungal / Q000235:genetics*; D012335:RNA, Ribosomal / Q000235:genetics*
D004271:DNA, Fungal; D004275:DNA, Ribosomal; D012331:RNA, Fungal; D012335:RNA, Ribosomal