DNA repair proficiency: a potential marker for identification of high risk members in breast cancer families.
Breast cancer is the single largest cancer and causes the high rate of cancer mortality among women. A positive family history of breast cancer is recognized as one of the major risk factors for this disease. The present study evaluates bleomycin (BLM)-induced chromosome sensitivity analysis in breast cancer families which provides indirectly a measure of the DNA repair defect of each person. BLM sensitivity assay on cultured lymphocytes of 36 familial breast cancer patients, their 85 first or second degree female relatives, 36 sporadic breast cancer patients and 40 age- and sex-matched controls (without any family history of cancer) were carried out to measure interindividual variation in their DNA repair capacity through mutagen-induced chromosome sensitivity analysis. Fifty percent of familial breast cancer patients and seven unaffected relatives showed hypersensitivity. Compared to hyposensitive relatives these seven subjects may be considered as high risk individuals.
Jyothish
B
B
Division of Cancer Research, Regional Cancer Centre, Thiruvananthapuram, India. rcctvm@md2.vsnl.net.in
Ankathil
R
R
Chandini
R
R
Vinodkumar
B
B
Nayar
G S
GS
Roy
D D
DD
Madhavan
J
J
Nair
M K
MK
D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000964:Antimetabolites, Antineoplastic / Q000494:pharmacology; D014408:Biomarkers, Tumor; D001761:Bleomycin / Q000494:pharmacology; D001943:Breast Neoplasms / Q000235:genetics*; D002842:Chromatids / Q000187:drug effects / Q000378:metabolism; D002871:Chromosome Banding; D002877:Chromosomes, Human / Q000187:drug effects; D004249:DNA Damage; D004260:DNA Repair; D004273:DNA, Neoplasm / Q000187:drug effects / Q000378:metabolism; D005192:Family Health; D005260:Female; D016195:G2 Phase / Q000502:physiology; D006801:Humans; D007206:Individuality; D008875:Middle Aged; D012307:Risk Factors; D016196:S Phase / Q000502:physiology
D000964:Antimetabolites, Antineoplastic; D014408:Biomarkers, Tumor; D004273:DNA, Neoplasm; D001761:Bleomycin