Production of strain specific antibodies against a synthetic polypeptide corresponding to the N-terminal region of the plum pox potyvirus coat protein.
Comparison of the predicted coat protein amino acid sequence of the 'sweet cherry' strain of plum pox potyvirus (PPV-SwC) with the corresponding regions of several other PPV strains indicated that the main differences are in the N-terminal region. Polyclonal antibodies were produced against a synthetic peptide corresponding to the 1-14 sequence of the N-terminal region of PPV-SwC coat protein. They specifically detected PPV-SwC in different immunochemical tests.
Crescenzi
A
A
Dipartimento di Biologia, Difesa e Biotecnologie Argo Forestali, Università degli Studi della Basilicata, Potenza, Italy. crescenzi@unibas.it
d'Aquino
L
L
Nuzzaci
M
M
Ostuni
A
A
Bavoso
A
A
Comes
S
S
De Stradis
A
A
Piazzolla
P
P
D000595:Amino Acid Sequence; D000914:Antibodies, Viral / Q000096:biosynthesis* / Q000276:immunology; D015153:Blotting, Western; D002213:Capsid / Q000737:chemistry / Q000276:immunology*; D036022:Capsid Proteins; D003001:Cloning, Molecular; D004797:Enzyme-Linked Immunosorbent Assay; D016253:Microscopy, Immunoelectron; D008969:Molecular Sequence Data; D010446:Peptide Fragments / Q000138:chemical synthesis / Q000737:chemistry / Q000276:immunology*; D010935:Plant Diseases / Q000821:virology; D010936:Plant Extracts; D017800:Potyvirus / Q000276:immunology* / Q000302:isolation & purification*; D017421:Sequence Analysis; D013045:Species Specificity
D000914:Antibodies, Viral; D036022:Capsid Proteins; D010446:Peptide Fragments; D010936:Plant Extracts; C095320:coat protein, Plum pox virus