Effect of a monoclonal antibody against interleukin-4 on collagen-induced arthritis in mice.
1 The present study was undertaken to investigate the effect of a monoclonal antibody (11B11 mAb) against interleukin-4 (IL-4) on collagen-induced arthritis (CIA) in mice. 2 11B11 mAb was daily injected intraperitoneally over a period of 10 days, commencing on the day of immunization with type II collagen (CII). 3 The results showed that the anti-IL-4 mAb markedly augmented both the incidence and the severity of CIA. The augmentation of the disease was associated with a significant increase in anti-CII IgG2a antibody production, proliferative responses of lymph node cells to CII and interferon-gamma (IFN-gamma) secretion from the lymphoid cells. The production of anti-CII IgG1 antibodies the secretion of IL-4 was markedly reduced in the mAb-treated mice. 4 Thus, the neutralization of IL-4 by 11B11 mAb appears to be effective in augmenting CIA.
Yoshino
S
S
Department of Microbiology, Saga Medical School, Nabeshima, Japan.
D000818:Animals; D000911:Antibodies, Monoclonal / Q000494:pharmacology*; D000917:Antibody Formation / Q000187:drug effects; D001169:Arthritis, Experimental / Q000276:immunology / Q000628:therapy*; D003094:Collagen; D005260:Female; D006968:Hypersensitivity, Delayed / Q000276:immunology; D007371:Interferon-gamma / Q000378:metabolism; D015847:Interleukin-4 / Q000276:immunology* / Q000378:metabolism; D008198:Lymph Nodes / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D008213:Lymphocyte Activation / Q000187:drug effects; D051379:Mice; D008811:Mice, Inbred DBA
D000911:Antibodies, Monoclonal; D015847:Interleukin-4; D007371:Interferon-gamma; D003094:Collagen