Immunoaffinity purification of the human multisubunit transcription factor IIH.
A procedure to immunoaffinity purify the human transcription factor IIH (TFIIH) was developed using a monoclonal antibody that recognizes an epitope in ERCC3 (XPB), the largest subunit of TFIIH. The epitope recognized by the monoclonal antibody was mapped to 20 amino acids. A peptide containing the epitope was capable of displacing TFIIH from an immunoaffinity column containing the monoclonal antibody. The immunoaffinity purification procedure described allows a simple and efficient method to purify both the "core" and "holo" TFIIH complexes.
LeRoy
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Howard Hughes Medical Institute, Division of Nucleic Acid Enzymology, Department of Biochemistry, Robert Wood Johnson Medical School, University of Medicine and Dentistry of New Jersey, Piscataway, New Jersey 08854-5635, USA.
Drapkin
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Weis
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L
Reinberg
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D
D000911:Antibodies, Monoclonal / Q000276:immunology; D002846:Chromatography, Affinity / Q000379:methods*; D018604:Epitope Mapping; D000939:Epitopes / Q000276:immunology; D006801:Humans; D051758:Transcription Factor TFIIH; D014157:Transcription Factors / Q000737:chemistry / Q000276:immunology / Q000302:isolation & purification*; D019473:Transcription Factors, TFII
D000911:Antibodies, Monoclonal; D000939:Epitopes; D014157:Transcription Factors; D019473:Transcription Factors, TFII; D051758:Transcription Factor TFIIH