title: Pediatric peripheral blood progenitor cell collection: haemonetics MCS 3P versus COBE Spectra versus Fresenius AS104.
abstract: BACKGROUND
  An increasing number of apheresis machines are becoming available for peripheral blood progenitor cell (PBPC) collection in children.
  
  
  METHODS
  At the Children's Hospital of Florence (Italy), three apheresis machines were evaluated: MCS 3P (Haemonetics) (10 procedures in 4 patients, aged 10-12 years, weight 23.5-64 kg), Spectra, (COBE) (8 procedures in 3 patients, aged 4-17 years, weight 19-59 kg), and AS104 (Fresenius) (24 procedures in 9 patients, aged 2-16 years, weight 13.6-60 kg). For PBPC quantitative analysis, CD34 cytofluorimetry was employed. Relevant variables analyzed included efficiency of CD34+ cell extraction and enrichment, mononuclear cell purity and red cell contamination of the apheresis components, and platelet count decreases after leukapheresis.
  
  
  RESULTS
  No significant differences in CD34+ cell-extraction abilities were found. However, the AS104 provided consistently purer leukapheresis components in terms of mononuclear cell and CD34+ cell enrichment (441 +/- 59%, vs. 240 +/- 35% and 290 +/- 42% for MCS 3P and Spectra, respectively). Postapheresis platelet counts dropped the least with the AS104. The smallest patient who underwent apheresis with MCS 3P (the only machine working on discontinuous flow and hence with greater volume shifts) weighed 23.5 kg and tolerated the procedure well, with no signs of hemodynamic instability. No significant complications were observed.
  
  
  CONCLUSIONS
  All machines seem to have comparable PBPC extraction efficiency, but the AS104 seems to give the component with the greatest PBPC enrichment. This feature might be relevant for further ex vivo cell processing (CD34+ cell selection, expansion, and so on).
authors.affiliation: Department of Pediatrics, University of Florence Azienda Ospedale A. Meyer, Italy.
authors.forename: F
authors.initials: F
authors.lastname: Bambi
authors.forename: L B
authors.initials: LB
authors.lastname: Faulkner
authors.forename: C
authors.initials: C
authors.lastname: Azzari
authors.forename: A M
authors.initials: AM
authors.lastname: Gelli
authors.forename: A
authors.initials: A
authors.lastname: Tamburini
authors.forename: V
authors.initials: V
authors.lastname: Tintori
authors.forename: A A
authors.initials: AA
authors.lastname: Lippi
authors.forename: F
authors.initials: F
authors.lastname: Tucci
authors.forename: G
authors.initials: G
authors.lastname: Bernini
authors.forename: F
authors.initials: F
authors.lastname: Genovese
mesh_terms: D000293:Adolescent; D018952:Antigens, CD34 / Q000097:blood; D001792:Blood Platelets / Q000166:cytology; D002648:Child; D002675:Child, Preschool; D005260:Female; D006412:Hematopoietic Stem Cells / Q000166:cytology*; D006801:Humans; D007937:Leukapheresis / Q000295:instrumentation*; D008297:Male; D010976:Platelet Count
chemical_list: D018952:Antigens, CD34