Distribution of olfactory receptor genes in the human genome.
We demonstrate that members of the olfactory receptor (OR) gene family are distributed on all but a few human chromosomes. Through FISH analysis, we show that OR sequences reside at more than 25 locations in the human genome. Their distribution is biased for terminal bands. Flow-sorted chromosomes were used to isolate 87 OR sequences derived from 16 chromosomes. Their sequence-relationships are indicative of the inter- and intrachromosomal duplications responsible for OR family expansion. The human genome has accumulated a striking number of dysfunctional copies: 72% of the sequences are pseudogenes. ORF-containing sequences predominate on chromosomes 7, 16 and 17.
Rouquier
S
S
CRBM, CNRS ERS 155, Montpellier, France.
Taviaux
S
S
Trask
B J
BJ
Brand-Arpon
V
V
van den Engh
G
G
Demaille
J
J
Giorgi
D
D
D000595:Amino Acid Sequence; D001483:Base Sequence; D002874:Chromosome Mapping; D002877:Chromosomes, Human; D002886:Chromosomes, Human, Pair 17; D003001:Cloning, Molecular; D017124:Conserved Sequence; D017931:DNA Primers; D005821:Genetic Techniques; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D007438:Introns; D008969:Molecular Sequence Data; D005810:Multigene Family; D018035:Receptors, Odorant / Q000235:genetics*; D017421:Sequence Analysis; D017386:Sequence Homology, Amino Acid
D017931:DNA Primers; D018035:Receptors, Odorant