Assessment of chromosomal gains and losses in oral squamous cell carcinoma by comparative genomic hybridisation.
Cytogenetic studies have demonstrated that oral squamous cell carcinomas (OSCCs) are usually characterised by complex karyotypes with many marker chromosomes. We analysed the genetic changes of six OSCC cell cultures by comparative genomic hybridisation (CGH). The CGH technique provides information on chromosomal gains and losses of the whole tumour genome in a single experiment and can therefore identify regions that harbour putative tumour suppressor genes (in the case of loss of chromosomal material) or oncogenes (in the case of gain or amplification of chromosomal material). Recurrent losses were detected at chromosome arms Xp and 3p (four cases). Gains consistently occurred at chromosome arms 8q and 9q (four cases) and at 1q, 3q, 5p, 7p, and 9p (three cases). The same six tumour cultures have previously been analysed by classical karyotyping. An important discrepancy between the two techniques was the number of losses detected: 55 with karyotyping versus 26 with CGH. On the basis of the cytogenetic complexity of these tumours and on FISH experiments that confirmed the CGH results, we conclude that genetic changes, particularly losses, can be more reliably detected by CGH analysis.
Hermsen
M A
MA
Department of Human Genetics, Free University, Amsterdam, The Netherlands.
Joenje
H
H
Arwert
F
F
Braakhuis
B J
BJ
Baak
J P
JP
Westerveld
A
A
Slater
R
R
D002294:Carcinoma, Squamous Cell / Q000235:genetics*; D002872:Chromosome Deletion; D002893:Chromosomes, Human, Pair 3; D002899:Chromosomes, Human, Pair 9; D004273:DNA, Neoplasm / Q000235:genetics*; D005260:Female; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D009062:Mouth Neoplasms / Q000235:genetics*; D009693:Nucleic Acid Hybridization; D014407:Tumor Cells, Cultured
D004273:DNA, Neoplasm