title: Local ileal cytokine responses in cattle during a primary infection with Cryptosporidium parvum.
abstract: In the present study, localized changes in cytokine transcription profiles were examined in neonatal calves following a primary infection with Cryptosporidium parvum, using competitive reverse transcriptase polymerase chain reaction (RT-PCR). Total RNA was prepared from ileocecal lymph nodes (LN), lamina propria lymphocytes (LPL), and intraepithelial lymphocytes (IEL) isolated from neonatal calves 7 days after C. parvum infection. Competitive RT-PCR performed on cDNA samples containing internal cytokine gene competitor molecules showed increases in the levels of interferon-gamma and interleukin-12 (IL-12) (P40) mRNA in both LPL and IEL populations but not in the draining LN. In addition, the levels of mRNA of the newly identified growth factor IL-15 decreased in the IEL of the infected animals. No consistent differences were seen in any of the cell populations when the samples were analyzed for IL-10 and levels of mRNA for IL-2 and IL-4 were low and highly variable in both infected and control groups in all 3 lymphocyte populations.
authors.affiliation: Immunology and Disease Resistance Laboratory, LPSI, ARS, USDA, Beltsville, Maryland 20705, USA.
authors.forename: A
authors.initials: A
authors.lastname: Canals
authors.forename: P
authors.initials: P
authors.lastname: Pasquali
authors.forename: D S
authors.initials: DS
authors.lastname: Zarlenga
authors.forename: R
authors.initials: R
authors.lastname: Fayer
authors.forename: S
authors.initials: S
authors.lastname: Almeria
authors.forename: L C
authors.initials: LC
authors.lastname: Gasbarre
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D002417:Cattle; D002418:Cattle Diseases / Q000276:immunology*; D003457:Cryptosporidiosis / Q000276:immunology / Q000662:veterinary*; D016785:Cryptosporidium parvum / Q000235:genetics / Q000276:immunology*; D016207:Cytokines / Q000096:biosynthesis* / Q000235:genetics; D007082:Ileum / Q000276:immunology*; D008198:Lymph Nodes / Q000166:cytology / Q000276:immunology; D008214:Lymphocytes / Q000276:immunology; D008297:Male; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000032:analysis
chemical_list: D016207:Cytokines; D012333:RNA, Messenger