title: Electron crystallography of yeast RNA polymerase II preserved in vitreous ice.
abstract: Two-dimensional (2-D) crystals of yeast RNA polymerase preserved in vitreous ice were studied by electron crystallographic and single-particle techniques. An electron density projection map of the enzyme was calculated from the data, which extended to a resolution of about 12 A, but was unexpectedly weak at resolutions higher than about 20 A. Multivariate statistics analysis revealed a large amount of variability in unit-cell structure in the polymerase crystals, partially related to high mobility of certain polymerase domains. Those same domains were previously identified as being involved in a conformational transition in the enzyme that controls DNA processivity and access to the active center cleft. Electron microscopic studies of other large multiprotein complexes are likely to require similar approaches to those described here.
authors.affiliation: Department of Structural Biology, Stanford University School of Medicine, CA 94305-5400, USA. asturias@cellbio.stanford.edu
authors.forename: F J
authors.initials: FJ
authors.lastname: Asturias
authors.forename: W
authors.initials: W
authors.lastname: Chang
authors.forename: Y
authors.initials: Y
authors.lastname: Li
authors.forename: R D
authors.initials: RD
authors.lastname: Kornberg
mesh_terms: D003461:Crystallography / Q000379:methods*; D007053:Ice; D007091:Image Processing, Computer-Assisted; D008854:Microscopy, Electron; D015999:Multivariate Analysis; D012319:RNA Polymerase II / Q000648:ultrastructure*; D016707:Tissue Fixation / Q000379:methods*; D015003:Yeasts / Q000737:chemistry*
chemical_list: D007053:Ice; D012319:RNA Polymerase II