title: Decreased corticosteroid-binding globulin in burn patients: relationship with interleukin-6 and fat in nutritional support.
abstract: OBJECTIVE
  To analyze the effect of low-fat nutritional solutions, with or without fish oil, on serum interleukin (IL)-6, and to explore the relationships between IL-6, corticosteroid-binding globulin (CBG; the main cortisol carrier in plasma), and protein metabolism in severely burned adult patients.
  
  
  METHODS
  Randomized, double-blind study with control and low fat-fed groups.
  
  
  METHODS
  Burn center of Hôtel-Dieu Hospital of Montréal.
  
  
  METHODS
  Thirty-seven men and women with thermal burn injury over >20% of body surface area and no other known medical condition.
  
  
  METHODS
  Within 24 hrs after admission, nutritional support was started through a gastroenteral tube inserted under endoscopic guidance. The goal for energy intake was calculated using the Curreri formula, and was adjusted with biweekly measurements of resting energy expenditure. Patients were randomly assigned to one of the following groups: control (35% of energy as fat); low fat 1 (15% of energy as fat); and low fat 2 (50% of fat in the form of fish oil).
  
  
  RESULTS
  Tumor necrosis factor (TNF)-alpha and TNF-beta, IL-6, CBG, and cortisol free fraction were measured every 3 days for 28 days. Nitrogen balance and urinary 3-methylhistidine excretion were measured daily. IL-6 concentrations were high in all patients, with the highest value (460 +/- 111 units/mL) observed on day 4. Concentrations of IL-6 were higher in control patients than in low fat-fed patients between days 13 and 28, but not between days 1 and 13. Multivariate analysis showed that IL-6, total body surface area burned, and sepsis scores were independent predictors of CBG between days 1 and 13 (n = 170; p<.00001). High IL-6 concentrations were predictors of low CBG concentrations and high cortisol free fractions. There was no relationship between IL-6, nitrogen balance, and 3-methylhistidine excretion. TNF-alpha and TNF-beta activity measurements by biological assay showed no correlation with other factors measured.
  
  
  CONCLUSIONS
  a) Low-fat feeding, with or without fish oil, does not change the early production of IL-6 after burn injury; b) serum IL-6 is negatively correlated with CBG, which supports the hypothesis that this cytokine inhibits hepatic CBG production; and c) IL-6 does not appear to directly influence protein metabolism in burn patients.
authors.affiliation: Centre Hospitalier de l'Université de Montréal, Centre des Grands Brûlés, PQ, Canada.
authors.forename: J
authors.initials: J
authors.lastname: Bernier
authors.forename: N
authors.initials: N
authors.lastname: Jobin
authors.forename: A
authors.initials: A
authors.lastname: Emptoz-Bonneton
authors.forename: M M
authors.initials: MM
authors.lastname: Pugeat
authors.forename: D R
authors.initials: DR
authors.lastname: Garrel
mesh_terms: D000328:Adult; D002056:Burns / Q000378:metabolism / Q000628:therapy*; D004041:Dietary Fats / Q000008:administration & dosage*; D004311:Double-Blind Method; D002149:Energy Intake; D004750:Enteral Nutrition; D005260:Female; D005395:Fish Oils / Q000008:administration & dosage; D005526:Food, Formulated; D006801:Humans; D006854:Hydrocortisone / Q000097:blood; D015850:Interleukin-6 / Q000097:blood; D008233:Lymphotoxin-alpha / Q000032:analysis; D008297:Male; D008762:Methylhistidines / Q000652:urine; D009584:Nitrogen / Q000378:metabolism; D010288:Parenteral Nutrition; D014156:Transcortin / Q000032:analysis*; D014409:Tumor Necrosis Factor-alpha / Q000032:analysis
chemical_list: D004041:Dietary Fats; D005395:Fish Oils; D015850:Interleukin-6; D008233:Lymphotoxin-alpha; D008762:Methylhistidines; D014409:Tumor Necrosis Factor-alpha; D014156:Transcortin; C028118:3-methylhistidine; D009584:Nitrogen; D006854:Hydrocortisone