title: Measurement of whole blood phagocyte chemiluminescence in the Wistar rat.
abstract: The factors influencing the rat whole blood chemiluminescence (CL): concentrations of blood, luminol, zymosan or opsonized zymosan, volume of the reaction mixture, storage time of blood samples and the presence of anticoagulants were evaluated. The CL micromethod described provides a fast and sensitive tool for the determination of metabolic activity of phagocytes in the microlitre range of rat whole blood.
authors.affiliation: Institute of Biophysics, Academy of Sciences of the Czech Republic. Brno, Czech Republic.
authors.forename: A
authors.initials: A
authors.lastname: Lojek
authors.forename: M
authors.initials: M
authors.lastname: Ciz
authors.forename: P
authors.initials: P
authors.lastname: Marnila
authors.forename: M
authors.initials: M
authors.lastname: Duskova
authors.forename: E M
authors.initials: EM
authors.lastname: Lilius
mesh_terms: D000818:Animals; D000925:Anticoagulants / Q000494:pharmacology; D001793:Blood Preservation; D001800:Blood Specimen Collection; D001810:Blood Volume; D007202:Indicators and Reagents; D007700:Kinetics; D008163:Luminescent Measurements; D008165:Luminol; D008297:Male; D010586:Phagocytes / Q000187:drug effects / Q000502:physiology*; D051381:Rats; D017208:Rats, Wistar; D012680:Sensitivity and Specificity; D015054:Zymosan
chemical_list: D000925:Anticoagulants; D007202:Indicators and Reagents; D008165:Luminol; D015054:Zymosan