RPG1: an essential gene of saccharomyces cerevisiae encoding a 110-kDa protein required for passage through the G1 phase.
In Saccharomyces cerevisiae cells a number of genes are required for progression through, or else to pass beyond, the G1 phase. We characterized a novel gene, RPG1, which is also involved in this phase. RPG1 is an essential gene encoding a 110-kDa evolutionarily conserved protein. Elutriated or alpha-factor-synchronized cells of the rpg1-1 temperature-sensitive mutant were arrested in the first cell cycle when shifted to a non-permissive temperature. The cells remained unbudded and neither grew nor duplicated DNA. rpg1-1 cells synchronized in S phase completed mitosis and arrested as unseparated G1 cells after a shift to a non-permissive temperature. Similarly, the asynchronous rpg1-1 cells accumulated in G1 at the non-permissive temperature, but mother and daughter cells did not separate. A bulk of Calcofluor-stained material was localized in the region adjacent to the cell septum. Our data show that Rpg1p is required for passage through the G1 phase and may be involved in growth control. Data published recently indicate that Rpg1p exhibits significant sequence similarity to a subunit of the mammalian translation initiation factor 3.
Kovarik
P
P
Vienna Biocenter, Institute of Biochemistry and Molecular Cell Biology of the University of Vienna, and Ludwig Boltzmann-Forschungsstelle für Biochemie, Vienna, Austria.
Hasek
J
J
Valásek
L
L
Ruis
H
H
D000595:Amino Acid Sequence; D018797:Cell Cycle Proteins / Q000737:chemistry / Q000235:genetics*; D004271:DNA, Fungal / Q000737:chemistry; D039621:Eukaryotic Initiation Factor-3; D005434:Flow Cytometry; D016193:G1 Phase; D008969:Molecular Sequence Data; D008970:Molecular Weight; D012441:Saccharomyces cerevisiae / Q000235:genetics*; D029701:Saccharomyces cerevisiae Proteins; D017422:Sequence Analysis, DNA; D013696:Temperature
D018797:Cell Cycle Proteins; D004271:DNA, Fungal; D039621:Eukaryotic Initiation Factor-3; C111889:RPG1 protein, S cerevisiae; D029701:Saccharomyces cerevisiae Proteins