[Immunohistochemistry of biomolecular markers of lung cancer].
104 surgery cases of non-small cell (NSLC) and small cell lung carcinoma (SLC) are studied. Oncoprotein bcl-2 is found in 49 out of 104 (47%) cases, more frequently in SLC (71%) than in NSLC (44%) and this correlated with carcinoma morphological malignancy. L-myc oncoprotein and EGFR were expressed practically in all cases, oncoprotein of the p53 mutated gene in 57% cases. The highest content of p53 was in SLC, large cell and poorly differentiated squamous cell carcinoma. Percentage of cells with mutated p53 statistically correlated with morphological malignancy of lung carcinoma. Oncoprotein of Rb gene was revealed in 51%, most frequently in squamous cell carcinoma (71%) and particularly in its well differentiated types. IGFII was found in 74% NSLC and in 100% SLC with cytoplasmic location in tumor cells; the level of expression was higher in SLC. IGFII 2 and 5 were more frequently observed in the foci of keratinization of squamous cell carcinoma. For the first time IGFP B3 was found not only in the cytoplasm but in the nuclei of tumor cells as well. There was a significant positive correlation between the content of IGFIBP3 in the nuclei of tumor cells and morphological malignancy (poor tumor cell differentiation, larger size and metastases). The mean number of proliferating Ki-67 positive cells was 24% but this figure was much higher (47%) in SLC. Squamous cell carcinoma is characterized by a more frequent and stronger expression of CD44 types 5 and 6 in the cytolemma and this may be considered as a marker of squamous cell differentiation of lung carcinoma.
Kogan
E A
EA
I.M. Sechenov Moscow Medical Academy.
Zhak
G
G
Kaĭzer
U
U
Shilli
M
M
Shurman
M
M
Sherer
A
A
Khavemann
K
K
D000328:Adult; D000368:Aged; D014408:Biomarkers, Tumor / Q000032:analysis*; D002289:Carcinoma, Non-Small-Cell Lung / Q000737:chemistry* / Q000175:diagnosis / Q000235:genetics; D018288:Carcinoma, Small Cell / Q000737:chemistry* / Q000175:diagnosis / Q000235:genetics; D015815:Cell Adhesion Molecules / Q000378:metabolism; D003937:Diagnosis, Differential; D005260:Female; D016147:Genes, Tumor Suppressor; D006133:Growth Substances / Q000378:metabolism; D006801:Humans; D007150:Immunohistochemistry; D008175:Lung Neoplasms / Q000737:chemistry* / Q000175:diagnosis / Q000235:genetics; D008297:Male; D008875:Middle Aged; D009857:Oncogenes
D014408:Biomarkers, Tumor; D015815:Cell Adhesion Molecules; D006133:Growth Substances
Immunogistokhimiia biomolekuliarnykh markerov raka legkogo.