title: Vinorelbine: cell cycle kinetics and differential sensitivity of human lymphocyte subpopulations.
abstract: Antiproliferative activity induced by vinorelbine (VRB) was studied in whole blood cultures (WBC) and in lymphocyte subpopulations evaluated by the MAC (morphology-antibody-chromosome) method. In WBC a significant delay in cell cycle kinetics in VRB-treated cultures compared with controls (P < 0.001) was observed. The highest dose (1.0 microg/ml) arrested all cells at the first metaphase. Both WBC and isolated lymphocyte cultures showed a significant increase in the mitotic index (MI) in VRB-treated cultures compared with controls (P < 0.001). Moreover, a significant increase in the MI was found in VRB-treated CD4 cells compared with VRB-treated CD8 (P < 0.01) and B-lymphocytes (P < 0.001).
authors.affiliation: Departamento de Genética, Instituto de Investigaciones Hematológicas Mariano R. Castex, Academia Nacional de Medicina, Buenos Aires, Argentina.
authors.forename: M
authors.initials: M
authors.lastname: González-Cid
authors.forename: I
authors.initials: I
authors.lastname: Larripa
authors.forename: I
authors.initials: I
authors.lastname: Slavutsky
mesh_terms: D000328:Adult; D000972:Antineoplastic Agents, Phytogenic / Q000494:pharmacology*; D002453:Cell Cycle; D005260:Female; D006801:Humans; D066298:In Vitro Techniques; D016131:Lymphocyte Subsets / Q000166:cytology / Q000187:drug effects*; D008297:Male; D008677:Metaphase; D008875:Middle Aged; D008940:Mitotic Index / Q000187:drug effects; D014747:Vinblastine / Q000031:analogs & derivatives* / Q000494:pharmacology; D000077235:Vinorelbine
chemical_list: D000972:Antineoplastic Agents, Phytogenic; D014747:Vinblastine; D000077235:Vinorelbine