Changes in soluble IL-6 receptor and IL-6 production by polymorphonuclear cells and whole blood cells of breast cancer patients.
The release of soluble interleukin 6 receptor (IL-6sR) and interleukin 6 (IL-6) was investigated in polymorphonuclear and whole blood cell cultures from untreated patients with breast cancer. IL-6sR and IL-6 were determined in the culture supernatants by a sensitive enzymoimmunological assay. We have shown a decreased ability of unstimulated and stimulated polymorphonuclear cells (PMNs) and whole blood cells (WBC) of cancer patients to release IL-6sR in comparison to a control group. Simultaneously, we have also found an increased capacity of unstimulated cells from the patients to produce of IL-6. The used stimulators-zymosan and lipopolysaccharide (LPS) resulted in increased IL-6sR secretion by PMNs and WBC of patients but did not influence IL-6 production by the same cells. We have also determined the levels of IL-6sR and IL-6 in the sera of cancer patients. The mean concentrations of IL-6sR and IL-6 in the sera of patients were higher than those in the control group. We have not noticed any correlations in the culture supernatant and serum levels of IL-6sR or IL-6 between control and cancer patient groups.
Jabłońska
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Department of Immunopathology, University Medical School, Białystok, Poland.
Pietruska
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D000328:Adult; D001773:Blood Cells / Q000276:immunology; D001943:Breast Neoplasms / Q000276:immunology*; D002277:Carcinoma / Q000276:immunology; D002455:Cell Division; D002478:Cells, Cultured; D005260:Female; D006801:Humans; D008222:Lymphokines / Q000096:biosynthesis*; D008875:Middle Aged; D009504:Neutrophils / Q000166:cytology / Q000276:immunology*; D019947:Receptors, Interleukin-6 / Q000096:biosynthesis*; D012995:Solubility
D008222:Lymphokines; D019947:Receptors, Interleukin-6