[DNA damages, their repair and apoptosis in the distant descendants of L5178Y(R) cells following radiation exposure (the DNA-comet method)].
Using a radioresistant subline of murine lymphoma cells L5178(R) DNA damages, their repair and the process of second DNA degradation in dynamics following X-radiation (5 Gy), and in the distant cell progeny have been studied by the method of DNA-comet assay (the alcaline conditions). The repair of DNA damages was found to be finished by 6 h, and by 18 h the second DNA-degradation was observed. The cell progeny was followed throughout 30 generations, with the enhancement of sensitivity to repeat irradiation, and the decrease in repair rate and degree of the second DNA degradation being registered. In the population of the 30th generation, the lack of the second DNA degradation was observed. If the second DNA degradation is indicative of apoptosis, as it is generally considered in literature, it can be supposed that in the distant progeny of irradiated L5178Y(R) cells radiation induces no programmed cell death and is not a trigger of apoptosis.
Afanas'ev
G G
GG
Institute of Chemical Physics, Russian Academy of Sciences, Moscow.
Iwaneńko
T
T
Kruszewski
M
M
Pelevina
I I
II
D000818:Animals; D017209:Apoptosis / Q000528:radiation effects*; D004249:DNA Damage / Q000528:radiation effects; D004260:DNA Repair / Q000528:radiation effects; D007940:Leukemia L5178 / Q000235:genetics* / Q000473:pathology*; D051379:Mice; D011836:Radiation Tolerance; D013997:Time Factors; D014407:Tumor Cells, Cultured
Povrezhdeniia DNK, ikh reparatsiia i apoptoz v otdalennykh pokoleniiakh kletok L5178Y(R) posle radiatsionnogo vozdeĭstviia (metod DNK-komet).