Targeted disruption of the ubiquitous CNC-bZIP transcription factor, Nrf-1, results in anemia and embryonic lethality in mice.
The CNC-basic leucine zipper (CNC-bZIP) family is a subfamily of bZIP proteins identified from independent searches for factors that bind the AP-1-like cis-elements in the beta-globin locus control region. Three members, p45-Nf-e2, Nrf-1 and Nrf-2 have been identified in mammals. Expression of p45-Nf-e2 is largely restricted to hematopoietic cells while Nrf-1 and Nrf-2 are expressed in a wide range of tissues. To determine the function of Nrf-1, targeted disruption of the Nrf-1 gene was carried out. Homozygous Nrf-1 mutant mice are anemic due to a non-cell autonomous defect in definitive erythropoiesis and die in utero.
Chan
J Y
JY
Department of Laboratory Medicine, University of California, San Francisco, CA 94143, USA.
Kwong
M
M
Lu
R
R
Chang
J
J
Wang
B
B
Yen
T S
TS
Kan
Y W
YW
D000595:Amino Acid Sequence; D000740:Anemia / Q000235:genetics*; D000818:Animals; D004268:DNA-Binding Proteins / Q000032:analysis / Q000235:genetics / Q000502:physiology*; D005314:Embryonic and Fetal Development; D004920:Erythropoiesis / Q000235:genetics*; D005333:Fetus; D005804:Genes, Lethal / Q000502:physiology*; D006720:Homozygote; D016350:Leucine Zippers; D008099:Liver; D051379:Mice; D018345:Mice, Knockout; D008969:Molecular Sequence Data; D051265:NF-E2-Related Factor 1; D051266:Nuclear Respiratory Factor 1; D051264:Nuclear Respiratory Factors; D012333:RNA, Messenger / Q000032:analysis; D015534:Trans-Activators / Q000032:analysis / Q000235:genetics / Q000502:physiology*
D004268:DNA-Binding Proteins; D051265:NF-E2-Related Factor 1; C495634:Nrf1 protein, mouse; D051266:Nuclear Respiratory Factor 1; D051264:Nuclear Respiratory Factors; D012333:RNA, Messenger; D015534:Trans-Activators