Study on the pathogenesis of porcine serum-induced liver fibrosis in rats with special reference to the effects of hypertension.
To investigate the pathogenesis of porcine serum (PS)-induced liver fibrosis in rats, two experiments were carried out, taking into consideration of hypertension and vascular changes. In Experiment I, spontaneous hypertensive rats (SHRs), two-kidney, one clip hypertensive F344 rats (2K1C rats), and normotensive F344 rats were given an intraperitoneal injection of PS of 0.5 ml twice a week for 8 weeks. Histopathological, immunohistochemical, and electron microscopical examinations were performed on the liver from each rat. Histological features of liver fibrosis in hypertensive and normotensive rats were essentially identical. However, in the PS-treated SHRs, 2 of 5 animals showed the most severe fibrosis in all PS-treated groups. Electron microscopically, degranulated mast cells, eosinophils, and macrophages engulfing apoptotic cells were rarely observed in the late stage of fibrous septa (FS) in the PS-treated SHR liver. In Experiment II with normotensive F344 rats, histopathological features of early FS in the liver were compared with those of late FS observed in Experiment I using serial sections, and we found that FS developed along the wall of newly formed vessels to connect between neighboring central veins. However, the effect of hypertension on this fibrosis could not be clearly demonstrated in the present study using SHRs and 2K1C rats.
Shiga
A
A
Department of Veterinary Pathology, School of Veterinary Medicine, Azabu University, Sagamihara, Kanagawa, Japan.
Shirota
K
K
Nishita
T
T
Nomura
Y
Y
D000818:Animals; D001803:Blood Transfusion; D006975:Hypertension, Portal / Q000473:pathology / Q000503:physiopathology*; D008106:Liver Cirrhosis, Experimental / Q000473:pathology / Q000503:physiopathology*; D008297:Male; D051381:Rats; D011916:Rats, Inbred F344; D011918:Rats, Inbred SHR; D012077:Renal Artery; D013552:Swine; D014183:Transplantation, Heterologous