Human T-cell leukemia virus type 1 Tax protein induces the expression of lymphocyte chemoattractant SDF-1/PBSF.
We investigated the mechanism of lymphocyte infiltration into tissues infected with human T-cell leukemia virus type 1 (HTLV-1). The cytokine SDF-1/PBSF is a highly efficient chemoattractant for lymphocytes. Reverse transcription-PCR analysis showed that among various human T-cell lines, those infected with HTLV-1 selectively expressed the SDF-1 gene. Expression of the viral protein Tax in a human T-cell line induced the expression of the SDF-1 gene, indicating that the constitutive expression of SDF-1 in virus-infected cell lines is at least in part mediated by Tax. HTLV-1-infected T-cell lines also expressed CXCR-4, a receptor for SDF-1. Moreover, chemotaxis assay showed that a HTLV-1-infected cell line migrated toward synthetic SDF-1. Thus, HTLV-1-infected cells are themselves responders for SDF-1. Our results suggest that SDF-1 induced by Tax may alter the distribution of HTLV-1-infected cells in vivo; hence it may contribute to their infiltration into affected tissues in HTLV-1-associated inflammatory diseases.
Arai
M
M
Department of Immunotherapeutics, Tokyo Medical and Dental University, Japan.
Ohashi
T
T
Tsukahara
T
T
Murakami
T
T
Hori
T
T
Uchiyama
T
T
Yamamoto
N
N
Kannagi
M
M
Fujii
M
M
D000818:Animals; D002461:Cell Line, Transformed; D054377:Chemokine CXCL12; D018925:Chemokines / Q000096:biosynthesis* / Q000235:genetics / Q000378:metabolism; D019743:Chemokines, CXC; D002630:Chemotactic Factors / Q000096:biosynthesis* / Q000235:genetics / Q000378:metabolism; D002634:Chemotaxis, Leukocyte; D016356:Gene Products, tax / Q000096:biosynthesis / Q000235:genetics / Q000378:metabolism*; D015368:Human T-lymphotropic virus 1 / Q000378:metabolism*; D006801:Humans; D019169:Jurkat Cells; D051379:Mice; D019718:Receptors, CXCR4 / Q000032:analysis; D013601:T-Lymphocytes / Q000378:metabolism* / Q000821:virology; D014407:Tumor Cells, Cultured
C515255:CXCL12 protein, human; D054377:Chemokine CXCL12; D018925:Chemokines; D019743:Chemokines, CXC; D002630:Chemotactic Factors; C515256:Cxcl12 protein, mouse; D016356:Gene Products, tax; D019718:Receptors, CXCR4