Removal of methylene blue from plasma via an adsorbent filter.
OBJECTIVE
To evaluate adsorbent filtration of methylene blue (MB) and leukocytes from plasma.


METHODS
Plasma (750 ml) from apheresis of 10 normal subjects was split into three aliquots: control (A), filtration (B) and MB addition (to 1 microM), phototreatment and filtration (C). Biochemical and coagulation tests were performed: units A and B were reinfused.


RESULTS
Filtration reduced MB to undetectable (< 0.05 microM) levels and leukocytes by 3 log10. Biochemical analytes were unchanged. The partial thromboplastin time was prolonged with MB addition (11 +/- 13%) or filtration (26 +/- 12%, p < 0.05), but the effects were not additive. Autologous transfusion was well tolerated.


CONCLUSIONS
Adsorbent filtration can reduce residual MB to undetectable levels and yields a component suitable for transfusion.
AuBuchon
J P
JP
Department of Pathology, Dartmouth-Hitchcock Medical Center, Lebanon, NH 03756, USA. james.p.aubuchon@hitchcock.org
Pickard
C
C
Herschel
L
L
O'Connor
J L
JL
Lee
E
E
D000327:Adsorption; D001804:Blood Transfusion, Autologous; D005260:Female; D005340:Fibrinogen / Q000032:analysis; D005374:Filtration / Q000295:instrumentation* / Q000379:methods; D006403:Hematologic Tests; D006801:Humans; D007958:Leukocyte Count; D008297:Male; D008751:Methylene Blue / Q000032:analysis*; D010314:Partial Thromboplastin Time; D010949:Plasma / Q000737:chemistry* / Q000821:virology; D011517:Prothrombin Time; D012016:Reference Values
D005340:Fibrinogen; D008751:Methylene Blue