Blood free radical antioxidant enzymes and lipid peroxides following long-distance and lactacidemic performances in highly trained aerobic and sprint athletes.
OBJECTIVE
We have determined the differences of the influence of prolonged exercise or higher intensity lactacidemic exercise, on plasma lipid peroxidation and on erythrocyte antioxidant enzymatic defence system.


METHODS
We measured plasma indices of lipid peroxidation, conjugated dienes (CD) and malondialdehyde (MDA) and erythrocyte enzymes superoxide dismutase (SOD), glutathione peroxidase (GSHPx) and catalase (CAT). The biochemical evaluations were performed in six healthy control males (C) and twelve athletes: six marathon runners (MR) and six sprint-trained athletes (STA) at rest and after a half-marathon (MR) and a training session of 6 x 150 m (STA).


RESULTS
In resting conditions MDA was higher in STA and MR than in C (p < 0.01), while only the MR showed significantly elevated levels of CD (p < 0.05). In STA the enzymatic scavenging capacity showed a significantly higher SOD (p < 0.01) and GSHPx (p < 0.01), while CAT was lower than in controls (p < 0.05). In MR only SOD (p < 0.01) was significantly higher than in C. It increased significantly immediately after half-marathon, while CAT decreased 24 and 48 hours postexercise respectively. In these athletes the lipoperoxidative indices increased in the early postexercise phase, while at 24 and 48 hrs both CD and MDA levels decreased. In STA enzyme activities were not modified by anaerobic performance while CD showed a peak 6 hrs postexercise and the MDA showed a progressive increase until 48 hrs afterwards.


CONCLUSIONS
Both strenuous long duration exercise and exhaustive sprint training overwhelm our capacity to detoxify ROS, producing oxidative stress. Thus an adequate supply of antioxidants could be appropriate.
Marzatico
F
F
Institute of Pharmacology, University of Pavia, Italy.
Pansarasa
O
O
Bertorelli
L
L
Somenzini
L
L
Della Valle
G
G
D000328:Adult; D000332:Aerobiosis; D000693:Anaerobiosis; D000704:Analysis of Variance; D000975:Antioxidants / Q000032:analysis*; D002374:Catalase / Q000097:blood*; D004912:Erythrocytes / Q000201:enzymology; D005500:Follow-Up Studies; D016166:Free Radical Scavengers / Q000097:blood*; D005979:Glutathione Peroxidase / Q000097:blood*; D006801:Humans; D019344:Lactic Acid / Q000097:blood*; D015227:Lipid Peroxidation; D008054:Lipid Peroxides / Q000097:blood*; D008297:Male; D008315:Malondialdehyde / Q000097:blood; D018384:Oxidative Stress / Q000502:physiology; D010807:Physical Endurance / Q000502:physiology; D017382:Reactive Oxygen Species / Q000378:metabolism; D012146:Rest / Q000502:physiology; D012420:Running / Q000502:physiology*; D013482:Superoxide Dismutase / Q000097:blood*
D000975:Antioxidants; D016166:Free Radical Scavengers; D008054:Lipid Peroxides; D017382:Reactive Oxygen Species; D019344:Lactic Acid; D008315:Malondialdehyde; D002374:Catalase; D005979:Glutathione Peroxidase; D013482:Superoxide Dismutase