Anti-IL-1 activity of peptide fragments of IL-1 family proteins.
A series of peptides containing retro-tuftsin- and tuftsin-like motifs from IL-1 proteins inhibits IL-1-induced IL-2 production and reduces the humoral immune response, thus supporting our hypothesis that tuftsin (Thr-Lys-Pro-Arg)-IL-1 competition depends on the presence of such motifs in IL-1 proteins. Some other peptides from regions of IL-1 responsible for receptor binding were also active, with peptide Ile-Thr-Gly-Ser-Glu (III) from IL-1alpha (residues 98-102), not only strongly affecting IL-2 production, but also suppressing the immune response; the analogue of hexapeptide Val-Thr-Lys-Phe-Tyr-Phe from the C-terminal part of IL-lra, with Lys replaced by Asp, was as efficient as Val-Thr-Lys-Phe-Tyr-Phe with respect to IL-1 competition.
Siemion
I Z
IZ
Faculty of Chemistry, University of Wrocław, Poland. SIEMION@WCHUWR.CHEM.UNI.WROC.PL
Kluczyk
A
A
Wieczorek
Z
Z
D000595:Amino Acid Sequence; D000818:Animals; D000917:Antibody Formation / Q000187:drug effects; D001667:Binding, Competitive; D002455:Cell Division / Q000187:drug effects; D002460:Cell Line; D066298:In Vitro Techniques; D053590:Interleukin 1 Receptor Antagonist Protein; D007375:Interleukin-1 / Q000037:antagonists & inhibitors* / Q000737:chemistry / Q000494:pharmacology*; D007376:Interleukin-2 / Q000096:biosynthesis; D051379:Mice; D008808:Mice, Inbred CBA; D010446:Peptide Fragments / Q000737:chemistry / Q000494:pharmacology*; D017472:Receptors, Interleukin-1 / Q000378:metabolism; D012795:Sialoglycoproteins / Q000737:chemistry / Q000494:pharmacology; D014405:Tuftsin / Q000737:chemistry / Q000494:pharmacology
C507764:Il1rn protein, mouse; D053590:Interleukin 1 Receptor Antagonist Protein; D007375:Interleukin-1; D007376:Interleukin-2; D010446:Peptide Fragments; D017472:Receptors, Interleukin-1; D012795:Sialoglycoproteins; D014405:Tuftsin