[The immunomodulating and immunoprotective activity of small ribonucleoprotein complexes (alpha RNPs) from the LRec-1sf cell line].
Effect of nuclear and released into culture medium alpha RNPs (N- and R-alpha-RNPs, resp.) produced by transformed rat embryo fibroblasts of serum-free cell line LRec-1sf on the nonsensibilized mouse splenocyte cytotoxicity (NK-mediated cell lysis) was studied. A preliminary treatment with N-alpha-RNPs resulted in decreasing K562 cell sensitivity to splenocyte cytotoxicity, whereas pretreatment of the splenocytes themselves exerted no cytotoxic effect. The target cell preincubation with R-alpha-RNPs had no influence on K562 cell resistance to NK cell cytotoxicity. The identical splenocyte preincubation was without action on their cytotoxic effect to LRec-1sf cells, however, resulted in an increase of the K562 cell lysis. The addition of R-alpha-RNPs into splenocyte/target cell mixtures had no influence on NK-mediated lysis, when K562 cells were used as a target cell line, but suppressed the NK-mediated lysis of LRec-1sf cells. The results of the present experiments suggest that alpha RNPs produced by LRec-1sf cell line exhibit the capacity for modulating both mouse NK cytotoxicity, and the transformed cell sensitivity to NK-mediated lysis.
Pleskach
V A
VA
Institute of Cytology, Russian Academy of Sciences, St. Petersburg.
Filatova
N A
NA
Kozhukharova
I V
IV
Il'kaeva
O R
OR
Atochina
O V
OV
Zenin
V V
VV
Konstaninova
I M
IM
D000276:Adjuvants, Immunologic / Q000302:isolation & purification / Q000494:pharmacology*; D000818:Animals; D002294:Carcinoma, Squamous Cell; D002460:Cell Line; D002461:Cell Line, Transformed / Q000276:immunology*; D002478:Cells, Cultured; D017077:Culture Media, Conditioned; D003602:Cytotoxicity, Immunologic / Q000187:drug effects; D004622:Embryo, Mammalian; D005347:Fibroblasts / Q000276:immunology; D006801:Humans; D007694:Killer Cells, Natural / Q000187:drug effects / Q000276:immunology; D051379:Mice; D008808:Mice, Inbred CBA; D051381:Rats; D017411:Ribonucleoproteins, Small Nuclear / Q000302:isolation & purification / Q000494:pharmacology*; D013154:Spleen / Q000166:cytology / Q000187:drug effects / Q000276:immunology; D014407:Tumor Cells, Cultured
D000276:Adjuvants, Immunologic; D017077:Culture Media, Conditioned; D017411:Ribonucleoproteins, Small Nuclear
Immunomoduliruiushchaia i immunoprotektivnaia aktivnost' malykh ribonukleoproteinovykh kompleksov (al'fa RNP) iz kletok linii LRec-1sf.