Structural studies of the O-antigen isolated from the phenol-soluble lipopolysaccharide of Acinetobacter baumannii (DNA group 2) strain 9.
A polysaccharide containing D-GalNAc, D-Glc and 4-acetamido-4,6-dideoxy-D-glucose (Qui4NAc) was isolated from the phenol-soluble lipopolysaccharide originating from Acinetobacter baumannii strain 9. The structure of the repeating unit was shown by means of monosaccharide analyses, Smith-degradation, partial acid hydrolysis, mass spectrometry, and NMR spectroscopy to be a branched pentasaccharide, in which the tetrasaccharide backbone is built from amino sugars only. [structure: see text] The polysaccharide was identified by serological and western blot analyses as the O-antigen of the lipopolysaccharide.
Haseley
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SR
Division of Medical and Biochemical Microbiology, Research Centre Borstel, Centre for Medicine and Biosciences, Germany.
Holst
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O
Brade
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H
D000150:Acinetobacter / Q000737:chemistry / Q000276:immunology*; D000818:Animals; D015153:Blotting, Western; D002240:Carbohydrate Sequence; D002241:Carbohydrates / Q000032:analysis; D004591:Electrophoresis, Polyacrylamide Gel; D007114:Immunization; D008070:Lipopolysaccharides / Q000737:chemistry; D009682:Magnetic Resonance Spectroscopy; D008969:Molecular Sequence Data; D019081:O Antigens / Q000737:chemistry*; D010636:Phenols; D011817:Rabbits; D012995:Solubility
D002241:Carbohydrates; D008070:Lipopolysaccharides; D019081:O Antigens; D010636:Phenols