The influence of two carotenoid food dyes on clastogenic activities of cyclophosphamide and dioxidine in mice.
The influence of the food dyes E160e (beta-apo-8'-carotenal in an oil suspension) and E160a (beta-carotene in an oil suspension) on clastogenic effects of cyclophosphamide (CP) and dioxidine (DN) was investigated. Chromosome damage in the bone marrow of C57BL/6 mice was reported. The following protocols were used: (1) simultaneous single administration of the dye and the mutagen and the subsequent animal sacrifice within 24 hr; (2) a 4-day pretreatment with the dye (daily administrations) followed with simultaneous injection of the dye and the mutagen on the 5th day 24 hr before sacrifice; (3) daily co-administration of the dye and the mutagen for 5 days with sacrifice 6 hr after the last administration. CP at a dose of 30 mg/kg and DN at 300 mg/kg were injected intraperitoneally; the dyes at doses of 0.5, 5 and 50 mg/kg were given orally. Under all the protocols applied, E160e at a dose of 50 mg/kg caused a significant reduction of both DN and CP effects. At 5 mg/kg this dye reduced the effects of the mutagens only under the pretreatment regimen. Pretreatment with E160a at doses of 5 and 50 mg/kg resulted in a meaningful reduction of the DN effect. Under the combined treatment with mutagens this dye reduced both CP and DN effects.
Durnev
A D
AD
Research Institute of Pharmacology, Russian Academy of Sciences, Moscow, Russia.
Tjurina
L S
LS
Guseva
N V
NV
Oreshchenko
A V
AV
Volgareva
G M
GM
Seredenin
S B
SB
D000818:Animals; D016587:Antimutagenic Agents / Q000494:pharmacology*; D000975:Antioxidants / Q000494:pharmacology; D001854:Bone Marrow Cells / Q000187:drug effects; D002338:Carotenoids / Q000494:pharmacology*; D003520:Cyclophosphamide / Q000037:antagonists & inhibitors / Q000633:toxicity*; D005505:Food Coloring Agents / Q000494:pharmacology*; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D009152:Mutagenicity Tests; D009153:Mutagens / Q000494:pharmacology*; D011810:Quinoxalines / Q000037:antagonists & inhibitors / Q000633:toxicity*; D019207:beta Carotene / Q000494:pharmacology*
D016587:Antimutagenic Agents; D000975:Antioxidants; D005505:Food Coloring Agents; D009153:Mutagens; D011810:Quinoxalines; C110652:beta-apo-8'-carotenal; D019207:beta Carotene; C001077:dioxidine; D002338:Carotenoids; D003520:Cyclophosphamide