Chelating effect of human serum proteins on metal-catalyzed ascorbate radical generation.
Purified human serum albumin and immunoglobulin G (IgG) were investigated for their metal-chelating activity, using ESR spectroscopy. Both copper (Cu2+) and iron ions (Fe3+) enhanced the radical intensity of both sodium ascorbate and sodium 5,6-benzylidene-L-ascorbate (SBA). Albumin significantly reduced the stimulation effect of copper, but not that of iron. On the other hand, IgG effectively reduced the radical intensity of iron, without affecting that of copper. The present study demonstrates the specific chelating action of these serum proteins, suggesting their possible preventive effects on metal-catalyzed pathogenic diseases.
Satoh
K
K
Analysis Center, School of Pharmaceutical Sciences, Showa University, Tokyo, Japan.
Ida
Y
Y
Kimura
S
S
Taguchi
K
K
Numaguchi
M
M
Gomi
K
K
Kochi
M
M
Sakagami
H
H
D000328:Adult; D000368:Aged; D000970:Antineoplastic Agents / Q000737:chemistry; D000975:Antioxidants / Q000737:chemistry; D001205:Ascorbic Acid / Q000031:analogs & derivatives* / Q000737:chemistry*; D001597:Benzylidene Compounds / Q000737:chemistry*; D002614:Chelating Agents; D003300:Copper; D003683:Dehydroascorbic Acid / Q000031:analogs & derivatives* / Q000737:chemistry; D005260:Female; D005312:Fetal Blood; D005609:Free Radicals / Q000737:chemistry; D006801:Humans; D007074:Immunoglobulin G; D007231:Infant, Newborn; D007501:Iron; D008297:Male; D008875:Middle Aged; D012709:Serum Albumin
D000970:Antineoplastic Agents; D000975:Antioxidants; D001597:Benzylidene Compounds; D002614:Chelating Agents; D005609:Free Radicals; D007074:Immunoglobulin G; D012709:Serum Albumin; C000820:semidehydroascorbic acid; D003300:Copper; D007501:Iron; D001205:Ascorbic Acid; C070680:zilascorb; D003683:Dehydroascorbic Acid