title: Development of the blood-labyrinth barrier in the rat.
abstract: Systemically administered cationic polyethyleneimine (PEI) passes through the capillary endothelial cell and attaches to anionic sites on the capillary basal lamina (BL). Thus, the distribution of PEI on the BL reflects the changes in the endothelial cell transport system. A 0.1% PEI solution was administered by intravenous injection (7.5 ml/kg) to developing (4, 7, 11, 14, 21 days after birth) and adult rats to evaluate the development of endothelial cell transport in the cochlear capillary as related to age. One hour later, the bony labyrinth was removed and embedded in Epoxy resin. Ultrathin sections of the cochlear lateral wall were then viewed with a transmission electron microscope. The distribution of PEI in the capillary BL in the stria vascularis of the 4-, 7- and 11-day-old rats was significantly greater compared to the adult rats. The distribution of PEI in the capillary BL in the spiral ligament of the 4- and 7-day-old rats was also significantly greater compared to the adult rats. These findings suggest that the endothelial cell transport is more robust in the developing rat cochlea and that the blood-labyrinth barrier becomes mature by 14 days after birth in rats.
authors.affiliation: Department of Otolaryngology, National Tohsei Hospital, Shizuoka, Japan.
authors.forename: M
authors.initials: M
authors.lastname: Suzuki
authors.forename: T
authors.initials: T
authors.lastname: Yamasoba
authors.forename: K
authors.initials: K
authors.lastname: Kaga
mesh_terms: D000818:Animals; D001485:Basement Membrane / Q000378:metabolism / Q000648:ultrastructure; D001693:Biological Transport, Active; D001790:Blood Physiological Phenomena; D002196:Capillaries / Q000378:metabolism / Q000648:ultrastructure; D003051:Cochlea / Q000098:blood supply / Q000254:growth & development / Q000502:physiology; D007758:Ear, Inner / Q000098:blood supply* / Q000254:growth & development* / Q000502:physiology; D004730:Endothelium, Vascular / Q000378:metabolism / Q000648:ultrastructure; D008854:Microscopy, Electron; D011094:Polyethyleneimine / Q000493:pharmacokinetics; D051381:Rats; D017208:Rats, Wistar
chemical_list: D011094:Polyethyleneimine