Postperfusion energy metabolism of steatotic graft and its relation to early graft viability following liver transplantation.
The present study was designed to assess energy metabolism of steatotic grafts and to determine its relation to early graft viability. Graft biopsies were taken, and the triglyceride content was determined in 29 grafts for the assessment of steatosis. The peak aspartate aminotransferase level and the concentrations of lactate and pyruvate were strongly correlated with the triglyceride content, suggesting that steatotic grafts are more vulnerable to preservation or reperfusion injury and that glucose oxidation is inhibited postoperatively in the steatotic grafts. Ketogenesis, an alternative pathway to produce energy substrates, was not accelerated even when the steatotic grafts produced more free carnitine to enhance the beta-oxidation of fatty acids. The deterioration of energy metabolism was associated with the increase in prothrombin time ratio, hepatocyte growth factor, and hyaluronic acid that reflected graft viability. Deterioration of postperfusion energy metabolism in the steatotic grafts may be involved in the development of irreversible graft damage.
Miki
C
C
Department of Surgery II, Mie University Medical School, Tsu, Japan.
Iriyama
K
K
Mirza
D F
DF
Mayer
A D
AD
Buckels
J A
JA
Suzuki
H
H
McMaster
P
P
D000328:Adult; D002331:Carnitine / Q000378:metabolism; D004734:Energy Metabolism / Q000502:physiology*; D005234:Fatty Liver / Q000378:metabolism*; D005260:Female; D006085:Graft Survival; D017228:Hepatocyte Growth Factor / Q000378:metabolism; D006801:Humans; D006820:Hyaluronic Acid / Q000378:metabolism; D007657:Ketone Bodies / Q000378:metabolism; D007773:Lactates / Q000378:metabolism; D008099:Liver / Q000378:metabolism; D016031:Liver Transplantation; D008297:Male; D011183:Postoperative Complications; D011517:Prothrombin Time; D011773:Pyruvates / Q000378:metabolism; D015424:Reperfusion; D000637:Transaminases / Q000378:metabolism; D014280:Triglycerides / Q000378:metabolism
D007657:Ketone Bodies; D007773:Lactates; D011773:Pyruvates; D014280:Triglycerides; D017228:Hepatocyte Growth Factor; D006820:Hyaluronic Acid; D000637:Transaminases; D002331:Carnitine