title: Newcastle disease outbreaks in recent years in western Europe were caused by an old (VI) and a novel genotype (VII).
abstract: Newcastle disease virus (NDV) strains, isolated from outbreaks during epizootics between 1992 and 1996 in Western European countries, were compared by restriction enzyme cleavage site mapping of the fusion (F) protein gene between nucleotides 334 and 1682 and by sequence analysis between nucleotides 47 and 435. Both methods revealed that NDV strains responsible for these epizootics belong to two distinct genotypes. Strains derived from sporadic cases in Denmark, Sweden, Switzerland and Austria were classified into genotype VI [6], the same group which caused outbreaks in the Middle East and Greece in the late 1960's and in Hungary in the early 1980's. In contrast, viruses that caused epizootics in Germany, Belgium, The Netherlands, Spain and Italy could be classified into a novel genotype (provisionally termed VII), hitherto undetected in Europe. It is possible that the genotype VII viruses originated in the Far East because they showed a high genetic similarity (97%) to NDV strains isolated from Indonesia in the late 1980's.
authors.affiliation: Veterinary Medical Research Institute, Hungarian Academy of Sciences, Budapest, Hungary.
authors.forename: B
authors.initials: B
authors.lastname: Lomniczi
authors.forename: E
authors.initials: E
authors.lastname: Wehmann
authors.forename: J
authors.initials: J
authors.lastname: Herczeg
authors.forename: A
authors.initials: A
authors.lastname: Ballagi-Pordány
authors.forename: E F
authors.initials: EF
authors.lastname: Kaleta
authors.forename: O
authors.initials: O
authors.lastname: Werner
authors.forename: G
authors.initials: G
authors.lastname: Meulemans
authors.forename: P H
authors.initials: PH
authors.lastname: Jorgensen
authors.forename: A P
authors.initials: AP
authors.lastname: Manté
authors.forename: A L
authors.initials: AL
authors.lastname: Gielkens
authors.forename: I
authors.initials: I
authors.lastname: Capua
authors.forename: J
authors.initials: J
authors.lastname: Damoser
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D018076:DNA, Complementary / Q000032:analysis / Q000235:genetics; D004196:Disease Outbreaks; D005060:Europe / Q000453:epidemiology; D005814:Genes, Viral / Q000235:genetics; D005838:Genotype; D008969:Molecular Sequence Data; D009521:Newcastle Disease / Q000453:epidemiology* / Q000821:virology*; D009522:Newcastle disease virus / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification; D010802:Phylogeny; D015183:Restriction Mapping; D017386:Sequence Homology, Amino Acid; D013045:Species Specificity; D014760:Viral Fusion Proteins / Q000235:genetics; D015678:Viral Structural Proteins / Q000235:genetics
chemical_list: D018076:DNA, Complementary; D014760:Viral Fusion Proteins; D015678:Viral Structural Proteins