Compensation for D-glutamate auxotrophy of Escherichia coli WM335 by D-amino acid aminotransferase gene and regulation of murI expression.
D-glutamate, an indispensable component of peptidoglycans of bacteria, is provided by glutamate racemase in E. coli cells. Compensation for D-glutamate auxotrophy of E. coli WM335 cells lacking the glutamate racemase gene, murI, with the D-amino acid aminotransferase gene suggests that presence of a threshold concentration for the D-glutamate required by E. coli cells, as well as a regulation system for murI expression.
Liu
L
L
Institute for Chemical Research, Kyoto University, Kyoto-fu, Japan.
Yoshimura
T
T
Endo
K
K
Kishimoto
K
K
Fuchikami
Y
Y
Manning
J M
JM
Esaki
N
N
Soda
K
K
D000410:Alanine Transaminase / Q000096:biosynthesis / Q000235:genetics / Q000378:metabolism*; D000591:Amino Acid Isomerases / Q000096:biosynthesis / Q000235:genetics / Q000378:metabolism*; D001216:Asparagine / Q000235:genetics / Q000378:metabolism; D003470:Culture Media / Q000378:metabolism / Q000494:pharmacology; D051263:D-Alanine Transaminase; D004926:Escherichia coli / Q000378:metabolism*; D015870:Gene Expression; D018698:Glutamic Acid / Q000378:metabolism* / Q000494:pharmacology; D008239:Lysine / Q000235:genetics / Q000378:metabolism; D016297:Mutagenesis, Site-Directed
D003470:Culture Media; D018698:Glutamic Acid; D001216:Asparagine; D000410:Alanine Transaminase; D051263:D-Alanine Transaminase; D000591:Amino Acid Isomerases; C062292:glutamate racemase; D008239:Lysine