Antiterminator protein GlpP of Bacillus subtilis binds to glpD leader mRNA.
The Bacillus subtilis glpD gene encodes glycerol-3-phosphate (G3P) dehydrogenase. Expression of glpD is mainly controlled by termination/antitermination of transcription at an inverted repeat in the glpD leader. Antitermination is mediated by the antiterminator protein GlpP in the presence of G3P. In this paper, interaction between GlpP and glpD leader mRNA in vivo and in vitro is reported. In vivo, the antiterminating effect of GlpP can be titrated in a strain carrying the glpD leader on a plasmid. GlpP has been purified and gel shift experiments have shown that it binds to glpD leader mRNA in vitro. GlpP is not similar to other known antiterminator proteins, but database searches have revealed an Escherichia coli ORF which has a high degree of similarity to GlpP.
Glatz
Elisabeth
E
Department of Microbiology, Lund University, Sölvegatan 12, S-223 62 Lund, Sweden.
Persson
Martin
M
Department of Microbiology, Lund University, Sölvegatan 12, S-223 62 Lund, Sweden.
Rutberg
Blanka
B
Department of Microbiology, Lund University, Sölvegatan 12, S-223 62 Lund, Sweden.
D001412:Bacillus subtilis / Q000235:genetics* / Q000378:metabolism*; D001426:Bacterial Proteins / Q000302:isolation & purification / Q000378:metabolism*; D001483:Base Sequence; D003001:Cloning, Molecular; D018388:Codon, Terminator; D004269:DNA, Bacterial / Q000235:genetics; D004926:Escherichia coli / Q000235:genetics; D015964:Gene Expression Regulation, Bacterial; D015971:Gene Expression Regulation, Enzymologic; D050536:Glycerol-3-Phosphate Dehydrogenase (NAD+); D005993:Glycerolphosphate Dehydrogenase / Q000235:genetics* / Q000378:metabolism*; D005994:Glycerophosphates / Q000378:metabolism; D008969:Molecular Sequence Data; D010957:Plasmids; D011485:Protein Binding; D012329:RNA, Bacterial / Q000096:biosynthesis / Q000378:metabolism; D012333:RNA, Messenger / Q000378:metabolism*; D014157:Transcription Factors; D014158:Transcription, Genetic; D014170:Transformation, Genetic
D001426:Bacterial Proteins; D018388:Codon, Terminator; D004269:DNA, Bacterial; D005994:Glycerophosphates; D012329:RNA, Bacterial; D012333:RNA, Messenger; D014157:Transcription Factors; C079802:glpP protein, Bacillus subtilis; D005993:Glycerolphosphate Dehydrogenase; D050536:Glycerol-3-Phosphate Dehydrogenase (NAD+)