Chemiluminescent immunoassay (CLIA) for salmon growth hormone (GH).
A highly sensitive and specific chemiluminescent immunoassay (CLIA) was developed for quantification of growth hormone (GH) in salmonid species. The CLIA for salmon GH was performed using the sandwich method with anti-GH IgG as the first antibody and chemiluminescent acridinium ester-labelled specific anti-GH F(ab')2 as the second antibody. The measurable range of salmon GH in the CLIA was 39-1250 pg/mL using a short assay (1 day) protocol and 3.9-125 pg/mL in a longer (2-day) assay. The dilution curve in the CLIA of serum from masu salmon (Oncorhynchus masou) was parallel to the standard curve of recombinant chum salmon (Oncorhynchus keta) GH. Seasonal changes of serum GH levels were measured in 1 year-old masu salmon cultivated in a pond from March to November. Their serum GH levels increased during smoltification from March to April, achieved a maximum level of 21 ng/mL in August, and then declined gradually to 11 ng/mL in October.
Fukada
H
H
Department of Biology and Aquaculture, Faculty of Fisheries, Hokkaido University, Japan.
Hiramatsu
N
N
Kitamura
M
M
Chiba
H
H
Hara
A
A
D000818:Animals; D013006:Growth Hormone / Q000097:blood*; D007118:Immunoassay / Q000379:methods; D007140:Immunoglobulin Fab Fragments; D008163:Luminescent Measurements; D017685:Oncorhynchus keta / Q000097:blood*; D011994:Recombinant Proteins / Q000032:analysis; D015203:Reproducibility of Results; D012474:Salmon / Q000097:blood*; D012621:Seasons; D012680:Sensitivity and Specificity
D007140:Immunoglobulin Fab Fragments; D011994:Recombinant Proteins; D013006:Growth Hormone