title: Localization of a tumour-suppressor gene associated with human oral cancer on 7q31.1.
abstract: To search for the existence of a tumour-suppressor gene (TSG) associated with oral squamous cell carcinoma (SCC), PCR analysis of microsatellite polymorphisms corresponding to 14 loci which map to chromosome 7q21.3-qter was performed to screen 35 patients with oral SCC for loss of heterozygosity (LOH). LOH was observed in at least one of the loci in 19 of 34 (55.9%) informative cases. Among the loci tested, frequent LOH was restricted at D7S522 on chromosome 7q31.1, which was measured within 1 cM. Furthermore, we detected microsatellite instability (MI) in 11 of 35 (31.4%) cases tested. Our observations indicate that alterations of chromosome 7q are associated with oral SCC tumorigenesis and that 7q31.1 might harbour at least one putative TSG.
authors.affiliation: Department of Oral Surgery, Chiba University School of Medicine, Japan. wangx-cib@umin.ac.jp
authors.forename: X L
authors.initials: XL
authors.lastname: Wang
authors.forename: K
authors.initials: K
authors.lastname: Uzawa
authors.forename: A
authors.initials: A
authors.lastname: Miyakawa
authors.forename: M
authors.initials: M
authors.lastname: Shiiba
authors.forename: T
authors.initials: T
authors.lastname: Watanabe
authors.forename: T
authors.initials: T
authors.lastname: Sato
authors.forename: T
authors.initials: T
authors.lastname: Miya
authors.forename: H
authors.initials: H
authors.lastname: Yokoe
authors.forename: H
authors.initials: H
authors.lastname: Tanzawa
mesh_terms: D002294:Carcinoma, Squamous Cell / Q000235:genetics*; D002897:Chromosomes, Human, Pair 7; D004273:DNA, Neoplasm / Q000235:genetics; D016147:Genes, Tumor Suppressor; D006801:Humans; D019656:Loss of Heterozygosity; D018895:Microsatellite Repeats; D009062:Mouth Neoplasms / Q000235:genetics*
chemical_list: D004273:DNA, Neoplasm