title: Inducible expression of the GLT-1 glutamate transporter in a CHO cell line selected for low endogenous glutamate uptake.
abstract: Inducible expression of the mammalian glial cell glutamate transporter GLT-1 has been established in a CHO cell line selected for low endogenous Na+-dependent glutamate uptake by [3H]aspartate suicide selection. Culturing the cells in doxycycline-containing medium, to activate GLT-1 expression via the Tet-On system, increased uptake of the GLT-1 substrate D-aspartate 280-fold, and increased cell size. Applying glutamate to whole-cell clamped, doxycycline-treated cells evoked a transporter-mediated current with characteristics appropriate for GLT-1. This cell line provides a useful tool for further examination of the electrical, biochemical and pharmacological properties of GLT-1, the most abundant glutamate transporter in the brain.
authors.affiliation: Department of Anatomy, University of Oslo, Norway.
authors.forename: L M
authors.initials: LM
authors.lastname: Levy
authors.forename: D
authors.initials: D
authors.lastname: Attwell
authors.forename: F
authors.initials: F
authors.lastname: Hoover
authors.forename: J F
authors.initials: JF
authors.lastname: Ash
authors.forename: M
authors.initials: M
authors.lastname: Bjørås
authors.forename: N C
authors.initials: NC
authors.lastname: Danbolt
mesh_terms: D018528:ATP-Binding Cassette Transporters / Q000096:biosynthesis* / Q000235:genetics; D027322:Amino Acid Transport System X-AG; D000818:Animals; D001692:Biological Transport; D016466:CHO Cells; D048429:Cell Size; D006224:Cricetinae; D004318:Doxycycline / Q000494:pharmacology; D018698:Glutamic Acid / Q000378:metabolism*; D012964:Sodium / Q000378:metabolism; D014162:Transfection
chemical_list: D018528:ATP-Binding Cassette Transporters; D027322:Amino Acid Transport System X-AG; D018698:Glutamic Acid; D012964:Sodium; D004318:Doxycycline