Mapping the gene for sex-linked anemia: an inherited defect of intestinal iron absorption in the mouse.
The sex-linked anemic (sla) mouse carries an anemia that results from an inherited defect of intestinal iron absorption and provides an ideal model with which to investigate this poorly understood yet clinically important process. We have precisely mapped the sla locus within the central region of the X chromosome in relation to a panel of microsatellite markers. Analysis of over 500 progeny from an intraspecific intercross and a smaller intraspecific backcross segregating sla established the following locus order in the sla region: DXMit45-sla- (DXMit16, DXMit96)-DXMit41-DXMit169-DXMit170- DXMit148-(DXMit18, DXMit171)-DXMit84-DXMit64. The two microsatellites DXMit16 and DXMit96 are located 0.60 +/- 0.35cM from sla and form the telomeric limit of the sla region. The mapping of the sla locus is an important first step to identifying the gene itself.
Anderson
G J
GJ
Clinical Sciences Unit, Queensland Institute of Medical Research, P.O. Royal Brisbane Hospital, Australia. gregA@qimr.edu.au
Murphy
T L
TL
Cowley
L
L
Evans
B A
BA
Halliday
J W
JW
McLaren
G D
GD
D018798:Anemia, Iron-Deficiency / Q000235:genetics* / Q000378:metabolism; D000818:Animals; D002874:Chromosome Mapping; D004276:DNA, Satellite; D004195:Disease Models, Animal; D008040:Genetic Linkage; D005819:Genetic Markers; D007413:Intestinal Mucosa / Q000378:metabolism*; D007501:Iron / Q000378:metabolism*; D051379:Mice; D008810:Mice, Inbred C57BL; D010641:Phenotype; D014960:X Chromosome
D004276:DNA, Satellite; D005819:Genetic Markers; D007501:Iron