title: Establishment of a novel cell line (TS-2) of pre-B acute lymphoblastic leukemia with a t(1;19) not involving the E2A gene.
abstract: The t(1;19)(q23;p13) translocation involving the E2A gene on chromosome 19p13.3 is a nonrandom translocation that is often seen in childhood pre-B-cell acute lymphoblastic leukemia (ALL). However, recent studies have demonstrated the presence of immunophenotypic and molecular heterogeneity among patients with the cytogenetically identical chromosome translocation. Here we report a novel pre-B ALL cell line, TS-2, with t(1;19) translocation not involving the E2A gene. The breakpoint of t(1;19) in TS-2 was demonstrated to be at 19p13.3, a region indistinguishable from the locus of the E2A gene, by cytogenetic study and fluorescence in situ hybridization. However, rearrangement of the E2A gene was not detected in TS-2 by Southern blot analysis. Moreover, the expressions of PBX1 or E2A/PBX1 fusion genes were not detected by an extensive study with Northern blot analysis and reverse transcription-polymerase chain reaction. These findings suggest that TS-2 may have a genetic abnormality involving uncharacterized gene(s) at 19p13.3 distinct from the E2A gene and, therefore, may be useful for investigating the heterogeneity of molecular pathogenesis in leukemias with t(1;19)(q23;p13) translocation.
authors.affiliation: Department of Pediatrics, Tohoku University School of Medicine, Sendai, Japan.
authors.forename: M
authors.initials: M
authors.lastname: Yoshinari
authors.forename: M
authors.initials: M
authors.lastname: Imaizumi
authors.forename: M
authors.initials: M
authors.lastname: Eguchi
authors.forename: M
authors.initials: M
authors.lastname: Ogasawara
authors.forename: T
authors.initials: T
authors.lastname: Saito
authors.forename: H
authors.initials: H
authors.lastname: Suzuki
authors.forename: Y
authors.initials: Y
authors.lastname: Koizumi
authors.forename: Y
authors.initials: Y
authors.lastname: Cui
authors.forename: A
authors.initials: A
authors.lastname: Sato
authors.forename: T
authors.initials: T
authors.lastname: Saisho
authors.forename: R
authors.initials: R
authors.lastname: Ichinohasama
authors.forename: Y
authors.initials: Y
authors.lastname: Matsubara
authors.forename: N
authors.initials: N
authors.lastname: Kamada
authors.forename: K
authors.initials: K
authors.lastname: Iinuma
mesh_terms: D017502:Adenovirus E2 Proteins / Q000235:genetics*; D015152:Blotting, Northern; D015139:Blotting, Southern; D002675:Child, Preschool; D002878:Chromosomes, Human, Pair 1; D002888:Chromosomes, Human, Pair 19; D004268:DNA-Binding Proteins / Q000235:genetics; D017809:Fatal Outcome; D005260:Female; D005434:Flow Cytometry; D018398:Homeodomain Proteins / Q000235:genetics; D006801:Humans; D007148:Immunoglobulin mu-Chains / Q000032:analysis; D016130:Immunophenotyping; D017404:In Situ Hybridization, Fluorescence; D007621:Karyotyping; D015514:Oncogene Proteins, Fusion / Q000235:genetics; D000075062:Pre-B-Cell Leukemia Transcription Factor 1; D054198:Precursor Cell Lymphoblastic Leukemia-Lymphoma / Q000235:genetics* / Q000276:immunology; D011518:Proto-Oncogene Proteins / Q000235:genetics; D014178:Translocation, Genetic; D014407:Tumor Cells, Cultured
chemical_list: D017502:Adenovirus E2 Proteins; D004268:DNA-Binding Proteins; D018398:Homeodomain Proteins; D007148:Immunoglobulin mu-Chains; D015514:Oncogene Proteins, Fusion; D000075062:Pre-B-Cell Leukemia Transcription Factor 1; D011518:Proto-Oncogene Proteins; C083400:PBX1 protein, human; C089847:E2A-Pbx1 fusion protein