Human herpesvirus 8 DNA in CNS lymphomas from patients with and without AIDS.
OBJECTIVE
Human herpesvirus 8 (HHV-8) has been found in association with Kaposi's sarcomas in human immunodeficiency virus (HIV)-positive and -negative patients, primary effusion lymphomas (PELs), multicentric Castleman's disease, and multiple myeloma. The PELs share several features with acquired immunodeficiency syndrome (AIDS)-associated primary central nervous system lymphomas (1 degree CNS-L), including B-cell phenotype, infection with Epstein-Barr virus, and lack of c-myc gene rearrangements. This prompted us to investigate the role of HHV-8 in 1 degree CNS-L and other brain lymphomas.


METHODS
To identify HHV-8, we performed nested and single polymerase chain reaction using DNA extracted from autopsy and biopsy brain lymphoma specimens of 36 patients with and without AIDS.


RESULTS
We detected HHV-8 DNA in 56% of all 1 degree CNS-L, with similar rates for patients with and without AIDS, but in just one of five metastatic B-cell lymphomas. Brain tissues histologically uninvolved by lymphoma were negative for HHV-8 in three patients in whom their 1 degree CNS-L was HHV-8 positive. In contrast, we easily demonstrated HHV-8 DNA in spleens and a KS lesion of patients with HHV-8-positive 1 degree CNS-L. All nonlymphoma brain controls were negative for HHV-8.


CONCLUSIONS
HHV-8 DNA is found in a significant percentage of 1 degree CNS-L, including patients with and without AIDS. Identification of HHV-8 in splenic tissue and absence of HHV-8 in control brain tissues suggests the possibility of a peripheral lymphocyte reservoir for HHV-8. Systemically infected lymphocytes may play a direct or indirect role in the pathogenesis of 1 degree CNS-L.
Corboy
J R
JR
Department of Neurology, University of Colorado Health Sciences Center, Denver 80262, USA.
Garl
P J
PJ
Kleinschmidt-DeMasters
B K
BK
D000328:Adult; D000368:Aged; D001932:Brain Neoplasms / Q000821:virology*; D016543:Central Nervous System Neoplasms / Q000821:virology*; D004279:DNA, Viral / Q000032:analysis*; D005260:Female; D015321:Gene Rearrangement; D016259:Genes, myc; D018023:HIV Seronegativity; D006679:HIV Seropositivity / Q000150:complications*; D006566:Herpesviridae Infections / Q000150:complications / Q000175:diagnosis*; D019288:Herpesvirus 8, Human / Q000235:genetics / Q000302:isolation & purification*; D006801:Humans; D008223:Lymphoma / Q000821:virology*; D016483:Lymphoma, AIDS-Related / Q000821:virology*; D016393:Lymphoma, B-Cell / Q000821:virology; D008297:Male; D008875:Middle Aged; D016133:Polymerase Chain Reaction; D012514:Sarcoma, Kaposi / Q000821:virology*
D004279:DNA, Viral