title: A large and distinct rotation of the myosin light chain domain occurs upon muscle contraction.
abstract: For more than 30 years, the fundamental goal in molecular motility has been to resolve force-generating motor protein structural changes. Although low-resolution structural studies have provided evidence for force-generating myosin rotations upon muscle activation, these studies did not resolve structural states of myosin in contracting muscle. Using electron paramagnetic resonance, we observed two distinct orientations of a spin label attached specifically to a single site on the light chain domain of myosin in relaxed scallop muscle fibers. The two probe orientations, separated by a 36 degrees +/- 5 degrees axial rotation, did not change upon muscle activation, but the distribution between them changed substantially, indicating that a fraction (17% +/- 2%) of myosin heads undergoes a large (at least 30 degrees) axial rotation of the myosin light chain domain upon force generation and muscle contraction. The resulting model helps explain why this observation has remained so elusive and provides insight into the mechanisms by which motor protein structural transitions drive molecular motility.
authors.affiliation: Department of Biochemistry, University of Minnesota Medical School, Minneapolis, MN 55455, USA.
authors.forename: J E
authors.initials: JE
authors.lastname: Baker
authors.forename: I
authors.initials: I
authors.lastname: Brust-Mascher
authors.forename: S
authors.initials: S
authors.lastname: Ramachandran
authors.forename: L E
authors.initials: LE
authors.lastname: LaConte
authors.forename: D D
authors.initials: DD
authors.lastname: Thomas
mesh_terms: D000818:Animals; D002118:Calcium / Q000494:pharmacology; D002645:Chickens; D004578:Electron Spin Resonance Spectroscopy; D008954:Models, Biological; D008974:Mollusca / Q000502:physiology*; D009068:Movement / Q000502:physiology*; D009119:Muscle Contraction / Q000502:physiology*; D018482:Muscle, Skeletal / Q000502:physiology; D009130:Muscle, Smooth / Q000502:physiology*; D018994:Myosin Light Chains / Q000502:physiology*; D011817:Rabbits; D013113:Spin Labels
chemical_list: D018994:Myosin Light Chains; D013113:Spin Labels; D002118:Calcium