The microtubule depolymerizing drugs nocodazole and colchicine inhibit the uptake of Listeria monocytogenes by P388D1 macrophages.
Uptake of Listeria monocytogenes by different mammalian cells like macrophages and epithelial cells is dependent on functional actin filaments and hence susceptible to inhibition by cytochalasin. Here we show that phagocytic uptake of L. monocytogenes by P388D1 macrophages is also highly sensitive to treatment with the microtubule depolymerizing drugs nocodazole and colchicine. This sensitivity is cell type specific and much less pronounced in bone marrow-derived macrophages and Caco-2 epithelial cells. In contrast to nocodazole and colchicine, the microtubule stabilizing drug taxol has no significant effect on the uptake of L. monocytogenes by all three cell types tested.
Kuhn
M
M
Theodor-Boveri-Institut für Biowissenschaften, Universität Würzburg, Lehrstuhl für Mikrobiologie, Germany. kuhn@biozentrum.uni-wuerzburg.de
D000199:Actins / Q000187:drug effects / Q000502:physiology; D000818:Animals; D000970:Antineoplastic Agents / Q000494:pharmacology*; D000972:Antineoplastic Agents, Phytogenic / Q000494:pharmacology; D018938:Caco-2 Cells / Q000382:microbiology; D003078:Colchicine / Q000494:pharmacology*; D003599:Cytoskeleton / Q000187:drug effects / Q000502:physiology; D003713:Dendritic Cells / Q000187:drug effects / Q000276:immunology / Q000382:microbiology; D006801:Humans; D007941:Leukemia P388 / Q000276:immunology*; D008089:Listeria monocytogenes / Q000276:immunology*; D008264:Macrophages / Q000187:drug effects / Q000276:immunology / Q000382:microbiology*; D051379:Mice; D008870:Microtubules / Q000187:drug effects / Q000502:physiology; D015739:Nocodazole / Q000494:pharmacology*; D017239:Paclitaxel / Q000494:pharmacology; D010587:Phagocytosis / Q000187:drug effects
D000199:Actins; D000970:Antineoplastic Agents; D000972:Antineoplastic Agents, Phytogenic; D017239:Paclitaxel; D015739:Nocodazole; D003078:Colchicine