title: Crystal structure of a cytokine-binding region of gp130.
abstract: The structure of the cytokine-binding homology region of the cell surface receptor gp130 has been determined by X-ray crystallography at 2.0 A resolution. The beta sandwich structure of the two domains conforms to the topology of the cytokine receptor superfamily. This first structure of an uncomplexed receptor exhibits a similar L-shaped quaternary structure to that of ligand-bound family members and suggests a limited flexibility in relative domain orientation of some 3 degrees. The putative ligand-binding loops are relatively rigid, with a phenylalanine side chain similarly positioned to exposed aromatic residues implicated in ligand binding for other such receptors. The positioning and structure of the N-terminal portion of the polypeptide chain have implications for the structure and function of cytokine receptors, such as gp130, which contain an additional N-terminal immunoglobulin-like domain.
authors.affiliation: Laboratory of Molecular Biophysics, The Rex Richards Building, South Parks Road, Oxford OX1 3QU.
authors.forename: J
authors.initials: J
authors.lastname: Bravo
authors.forename: D
authors.initials: D
authors.lastname: Staunton
authors.forename: J K
authors.initials: JK
authors.lastname: Heath
authors.forename: E Y
authors.initials: EY
authors.lastname: Jones
mesh_terms: D000595:Amino Acid Sequence; D015703:Antigens, CD / Q000737:chemistry*; D001665:Binding Sites; D017124:Conserved Sequence / Q000235:genetics; D018360:Crystallography, X-Ray; D050822:Cytokine Receptor gp130; D016207:Cytokines / Q000378:metabolism*; D006801:Humans; D008024:Ligands; D008562:Membrane Glycoproteins / Q000737:chemistry*; D008958:Models, Molecular; D008969:Molecular Sequence Data; D011487:Protein Conformation; D011993:Recombinant Fusion Proteins / Q000737:chemistry
chemical_list: D015703:Antigens, CD; D016207:Cytokines; C494199:IL6ST protein, human; D008024:Ligands; D008562:Membrane Glycoproteins; D011993:Recombinant Fusion Proteins; D050822:Cytokine Receptor gp130