title: Optimal timing for collection of PBPC after glycosylated G-CSF administration.
abstract: Daily administration of granulocyte colony-stimulating Factor (G-CSF) results in progenitor cell mobilization with maximum blood levels achieved after 4-7 days. In this study the short-term effects of glycosylated G-CSF at a dose of 5 microg/kg s.c. were determined so as to allow optimization of the timing of progenitor cell collection. In the first study involving 20 normal volunteers, a significant fall in neutrophil count and G-CSF levels was observed 2 h after the G-CSF injection. To investigate this phenomenon serial measurements were made in a further six volunteers after the 6th daily injection of G-CSF. A fall in the neutrophil count occurred which was maximal at 1 h and recovered to baseline within 3 h. There was also a fall in CD34+ cells (P = 0.034), GM-CFC (P = 0.025) and BFU-E (P = 0.066) and recovery to baseline levels took 4-12 h. We conclude that glycosylated G-CSF should not be given immediately prior to stem cell collection.
authors.affiliation: University College London Medical School, Central Middlesex Hospital, UK.
authors.forename: M J
authors.initials: MJ
authors.lastname: Watts
authors.forename: I
authors.initials: I
authors.lastname: Addison
authors.forename: S J
authors.initials: SJ
authors.lastname: Ings
authors.forename: S G
authors.initials: SG
authors.lastname: Long
authors.forename: S
authors.initials: S
authors.lastname: Hartley
authors.forename: S
authors.initials: S
authors.lastname: Warrington
authors.forename: M
authors.initials: M
authors.lastname: Boyce
authors.forename: D C
authors.initials: DC
authors.lastname: Linch
mesh_terms: D000328:Adult; D018952:Antigens, CD34 / Q000378:metabolism; D001781:Blood Component Removal / Q000379:methods*; D003114:Colony-Forming Units Assay; D006031:Glycosylation; D016179:Granulocyte Colony-Stimulating Factor / Q000008:administration & dosage* / Q000097:blood / Q000737:chemistry; D018380:Hematopoietic Stem Cell Transplantation; D006412:Hematopoietic Stem Cells / Q000166:cytology / Q000187:drug effects / Q000276:immunology; D006801:Humans; D007958:Leukocyte Count; D008297:Male; D009504:Neutrophils / Q000166:cytology / Q000187:drug effects / Q000276:immunology; D013997:Time Factors
chemical_list: D018952:Antigens, CD34; D016179:Granulocyte Colony-Stimulating Factor