title: An ethanol inducible gene switch for plants used to manipulate carbon metabolism.
abstract: Many transgenic plant studies use constitutive promoters to express transgenes. For certain genes, deleterious effects arise from constant expression in all tissues throughout development. We describe a chemically inducible plant gene expression system, with negligible background activity, that obviates this problem. We demonstrate its potential by showing inducible manipulation of carbon metabolism in transgenic plants. Upon rapid induction of yeast cytosolic invertase, a marked phenotype appears in developing leaves that is absent from leaves that developed before induction or after it has ceased.
authors.affiliation: School of Biological Sciences, University of Liverpool, Donnan Laboratories, UK.
authors.forename: M X
authors.initials: MX
authors.lastname: Caddick
authors.forename: A J
authors.initials: AJ
authors.lastname: Greenland
authors.forename: I
authors.initials: I
authors.lastname: Jepson
authors.forename: K P
authors.initials: KP
authors.lastname: Krause
authors.forename: N
authors.initials: N
authors.lastname: Qu
authors.forename: K V
authors.initials: KV
authors.lastname: Riddell
authors.forename: M G
authors.initials: MG
authors.lastname: Salter
authors.forename: W
authors.initials: W
authors.lastname: Schuch
authors.forename: U
authors.initials: U
authors.lastname: Sonnewald
authors.forename: A B
authors.initials: AB
authors.lastname: Tomsett
mesh_terms: D000426:Alcohol Dehydrogenase / Q000235:genetics*; D001233:Aspergillus nidulans / Q000235:genetics; D002244:Carbon / Q000378:metabolism*; D017796:Caulimovirus / Q000235:genetics; D004268:DNA-Binding Proteins / Q000235:genetics*; D000431:Ethanol / Q000494:pharmacology*; D005656:Fungal Proteins / Q000235:genetics*; D018506:Gene Expression Regulation, Plant / Q000187:drug effects*; D005822:Genetic Vectors; D006026:Glycoside Hydrolases / Q000096:biosynthesis; D010641:Phenotype; D010788:Photosynthesis; D030821:Plants, Genetically Modified; D010947:Plants, Toxic; D011401:Promoter Regions, Genetic; D018085:Regulon; D014026:Nicotiana / Q000201:enzymology / Q000235:genetics / Q000378:metabolism; D014157:Transcription Factors / Q000235:genetics*; D019076:Transgenes; D043324:beta-Fructofuranosidase
chemical_list: C074202:ALCR protein, Aspergillus nidulans; D004268:DNA-Binding Proteins; D005656:Fungal Proteins; D014157:Transcription Factors; D000431:Ethanol; D002244:Carbon; D000426:Alcohol Dehydrogenase; D006026:Glycoside Hydrolases; D043324:beta-Fructofuranosidase