title: New murine monoclonal antibodies directed against glycophorins C and D, have anti-Ge2 specificity.
abstract: OBJECTIVE
  The Gerbich blood group system comprises three high-incidence antigens which have been localized on glycophorins C (GPC) and D (GPD) molecules. Murine monoclonal antibodies (mAbs) directed against Ge4 and Ge3 epitopes have already been obtained. We describe two murine mAbs (ZI51.17 and J6) with Ge2 specificity.
  
  
  METHODS
  The mAbs were investigated by serological methods and immunoblotting, with common, Ge-negative and enzyme-modified RBCs.
  
  
  RESULTS
  Both mAbs behaved like anti-Ge2 in serological tests, but bound to GPC and GPD on immunoblots. ZI51.17 did not recognize the GPCYus or GPCGe variants, whereas J6 revealed a 25-kD component from Ge:-2,-3,4 membranes, which is identical to the GPCGe molecule.
  
  
  CONCLUSIONS
  These two mAbs are directed to slightly different epitopes. The ZI51.17 and J6 epitopes are common to GPC and GPD, thus distinct from the Ge2 antigen defined by human alloantibodies.
authors.affiliation: Etablissement de Transfusion Sanguine de l'Assistance Publique, Hôpitaux de Paris, Site St-Louis, France.
authors.forename: D
authors.initials: D
authors.lastname: Janvier
authors.forename: S
authors.initials: S
authors.lastname: Veaux
authors.forename: M
authors.initials: M
authors.lastname: Benbunan
mesh_terms: D000372:Agglutination Tests; D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D001789:Blood Group Antigens / Q000276:immunology*; D000939:Epitopes / Q000097:blood / Q000235:genetics; D005091:Exons; D006021:Glycophorins / Q000235:genetics / Q000276:immunology* / Q000378:metabolism; D006801:Humans; D015151:Immunoblotting; D051379:Mice; D008807:Mice, Inbred BALB C / Q000276:immunology*; D011485:Protein Binding
chemical_list: D000911:Antibodies, Monoclonal; D001789:Blood Group Antigens; D000939:Epitopes; D006021:Glycophorins