Heterogeneous induction of apoptosis in colon cancer cells by wild-type p53 gene transfection.
To examine the effects of wild-type (wt)-p53 gene transfer on cancer cell growth and apoptosis induction, we transduced human wt-p53 cDNA into three colon cancer cell lines either with or without a mutation of the p53 gene using the HVJ-cationic liposome method. Wt-p53 gene transfer, thus, induced an apparent growth arrest in all cell lines, but its enhancement of the apoptotic rate varied (from about 4 to 70 times). The simultaneous doxorubicin treatment was able to enhance growth arrest and the apoptosis induction rate. These findings suggest that wt-p53 gene transfer using HVJ-cationic liposomes seems to be a potentially effective therapeutic strategy, however wt-p53 gene transfer still appears to be more effective in combination with other cytotoxic treatments.
Namoto
M
M
First Department of Pathology, Faculty of Medicine, Kyushu University, Fukuoka 812-8582, Japan.
Yonemitsu
Y
Y
Nakagawa
K
K
Hashimoto
S
S
Kaneda
Y
Y
Nawata
H
H
Sueishi
K
K
D017209:Apoptosis; D003110:Colonic Neoplasms / Q000473:pathology* / Q000628:therapy / Q000648:ultrastructure; D053938:DNA Fragmentation; D004317:Doxorubicin / Q000494:pharmacology; D016158:Genes, p53 / Q000502:physiology*; D006801:Humans; D007150:Immunohistochemistry; D008854:Microscopy, Electron; D014162:Transfection; D014407:Tumor Cells, Cultured; D016159:Tumor Suppressor Protein p53 / Q000032:analysis
D016159:Tumor Suppressor Protein p53; D004317:Doxorubicin