ISRm4-1 and ISRm9, two novel insertion sequences from Sinorhizobium meliloti.
Two novel insertion sequences, ISRm4-1 and ISRm9 have been identified in Sinorhizobium meliloti. ISRm4-1 is 936-bp in length, flanked by 17-bp putative terminal inverted repeats and a putative target duplication of 3-bp. ISRm4-1 is a member of the IS5 family of insertion sequences, closely related to ISRm4. ISRm9 is 2797-bp in length and carries 25-bp inverted repeats with target duplication of 7-bp: ISRm9 belongs to the IS21 family of insertion elements. On the non-pSym plasmid pRmeGR4b from S. meliloti strain GR4, a copy of ISRm4-1 is interrupted at nucleotide 150 from its 5'-end by a copy of ISRm9. Whereas ISRm4-like elements are widespread in S. meliloti, the distribution of ISRm9 appears to be correlated to that of pRmeGR4b-type plasmids.
Zekri
S
S
Departamento de Microbiología del Suelo y Sistemas Simbióticos, Consejo Superior de Investigaciones Científicas, Granada, Spain.
Soto
M J
MJ
Toro
N
N
D000595:Amino Acid Sequence; D004251:DNA Transposable Elements; D008969:Molecular Sequence Data; D010957:Plasmids; D016962:Sinorhizobium meliloti / Q000235:genetics*
D004251:DNA Transposable Elements