Medium chains of adaptor complexes AP-1 and AP-2 recognize leucine-based sorting signals from the invariant chain.
Interactions between tyrosine- and leucine-based sorting signals in the cytoplasmic tails of transmembrane proteins and adaptor complexes AP-1 and AP-2 are believed to be the first step in the formation of clathrin-coated vesicles that deliver these proteins to their destination. Medium chains of AP-1 and AP-2 have been reported to interact with tyrosine-based sorting signals in a number of in vitro assays. In the present study we found that recombinant medium chains could interact with leucine-based sorting signals from the cytoplasmic tail of the invariant chain. Medium chains may therefore be responsible for the proper recognition of both tyrosine and leucine sorting signals by AP-1 and AP-2 complexes.
Rodionov
D G
DG
Division of Molecular Cell Biology, Department of Biology, University of Oslo, P. O. Box 1050 Blindern, 0316 Oslo, Norway.
Bakke
O
O
D033965:Adaptor Protein Complex alpha Subunits; D033942:Adaptor Proteins, Vesicular Transport; D000595:Amino Acid Sequence; D000944:Antigens, Differentiation, B-Lymphocyte / Q000378:metabolism*; D001665:Binding Sites; D001692:Biological Transport; D002451:Cell Compartmentation; D000949:Histocompatibility Antigens Class II / Q000378:metabolism*; D007930:Leucine / Q000378:metabolism; D008565:Membrane Proteins / Q000235:genetics / Q000378:metabolism*; D008969:Molecular Sequence Data; D010446:Peptide Fragments / Q000378:metabolism; D021382:Protein Sorting Signals / Q000235:genetics / Q000378:metabolism*; D011994:Recombinant Proteins / Q000378:metabolism
D033965:Adaptor Protein Complex alpha Subunits; D033942:Adaptor Proteins, Vesicular Transport; D000944:Antigens, Differentiation, B-Lymphocyte; D000949:Histocompatibility Antigens Class II; D008565:Membrane Proteins; D010446:Peptide Fragments; D021382:Protein Sorting Signals; D011994:Recombinant Proteins; C045084:invariant chain; D007930:Leucine