Revascularized intercalary bone allografts with short-term immunosuppression with cyclosporine in the canine.
To study the healing process of vascularized intercalary bone allograft after withdrawal of immunosuppressive drugs, allotransplantation of the tibia diaphysis with a vascular pedicle was performed in eight adult mongrel dogs (group 2) and assessments were made both during administration and after discontinuation of cyclosporin A. As controls, similar grafts with the vascular pedicles were removed and reimplanted back to the same animals (five dogs, group 1). Allotransplantation of frozen stored bone without a vascular pedicle (10 dogs, groups 3A and 3B) were also compared. No union occurred in most cases of frozen stored bone allotransplant because the transplanted bone was resorbed, leading to loosening and subsequent failure of osteosynthesis with the plate and screws used. Under cyclosporin A immunosuppression, bony union (i.e., when trabeculae were seen crossing the graft-recipient junction with obliteration of the junction line) occurred at almost similar time intervals in all dogs of group 2 (bone allotransplant with a vascular pedicle) by 3 months postoperatively, which was similar to those of group 1. No systemic side effects of cyclosporin A were observed. Cyclosporin A was discontinued 3 months following graft implantation. The bone graft became avascular within a week following withdrawal of cyclosporin A. However, bone union was maintained, and the transplanted bone never showed bone resorption, sclerosis, or fracture on serial radiographs up to the time the animals were sacrificed, between 5 and 14 months later. Histology at sacrifice showed that the transplanted allografts were being replaced at both ends by fresh bone derived from the transplantation bed. We conclude on the basis of the results of this study that solid bony union can be obtained in allotransplanted bone with a vascular pedicle if cyclosporin A is given for a brief period. After cyclosporin A is withdrawn, although the bone becomes nonviable secondary to rejection occurring in the blood vessels, its skeletal structure remains intact, enabling it to maintain its structural support while awaiting replacement by bony ingrowth from both ends of the graft.
Doi
K
K
Department of Orthopedic Surgery at the Ogori Daiichi General Hospital, Japan.
Akino
T
T
Shigetomi
M
M
Muramatsu
K
K
Kawai
S
S
D000818:Animals; D001860:Bone Plates; D001862:Bone Resorption / Q000209:etiology / Q000473:pathology; D001863:Bone Screws; D016025:Bone Transplantation / Q000379:methods* / Q000473:pathology; D015925:Cryopreservation; D016572:Cyclosporine / Q000627:therapeutic use*; D004285:Dogs; D004868:Equipment Failure; D005500:Follow-Up Studies; D050723:Fractures, Bone / Q000473:pathology; D006084:Graft Rejection / Q000209:etiology / Q000473:pathology; D006085:Graft Survival; D007166:Immunosuppressive Agents / Q000627:therapeutic use*; D010012:Osteogenesis; D010026:Osteosclerosis / Q000473:pathology; D012092:Replantation; D013524:Surgical Flaps / Q000098:blood supply; D013977:Tibia / Q000098:blood supply / Q000473:pathology / Q000601:surgery; D014182:Transplantation, Autologous; D014184:Transplantation, Homologous; D014945:Wound Healing
D007166:Immunosuppressive Agents; D016572:Cyclosporine