title: Developing rat brainstem motoneurones in organotypic culture express calcium permeable AMPA-gated receptors.
abstract: Recent studies suggest that calcium permeable excitatory amino acid receptors may play an important role in many developmental processes including dendritic differentiation, synaptogenesis and activity dependent plasticity or excitotoxicity related disorders. In this work, we investigate the existence of calcium permeable AMPA receptors in embryonic rat motoneurones in organotypic slice culture, which display an early sensitivity to AMPA and Kainate. We used excitatory amino acids induced cobalt uptake and show that developing motoneurones express Ca2+ permeable AMPA receptors. We demonstrate, as already described for other neuronal types in acute slices of adult animals, that the cobalt loading of motoneurones is not suppressed by blockade of synaptic activity by TTX. It is not induced by NMDA stimulation and does not result from the activation of voltage dependent calcium channels. It is specifically suppressed by the non-competitive AMPA antagonist GYKI 53784 (LY303070) and enhanced by the AMPA-receptor desensitization blocker Aniracetam. We conclude that cobalt loading results from the specific activation of AMPA receptors. We further show that, when cobalt loading is induced by threshold doses of Glutamate agonists, cobalt-sulfide deposits are found specifically in primary dendrites, dendritic spines and localized spots on the somatic and peripheral dendritic membrane. We suggest that this particular pattern of staining, different from the Golgi-like staining obtained with excitotoxic doses, may offer new information regarding the membrane density and distribution of calcium permeable AMPA receptors.
authors.affiliation: Unité de Neurocybernétique Cellulaire, CNRS UPR 9041, 280, Bd. Sainte Marguerite 13009, Marseille, France.
authors.forename: T
authors.initials: T
authors.lastname: Launey
authors.forename: A
authors.initials: A
authors.lastname: Ivanov
authors.forename: N
authors.initials: N
authors.lastname: Ferrand
authors.forename: J P
authors.initials: JP
authors.lastname: Gueritaud
mesh_terms: D000818:Animals; D001933:Brain Stem / Q000166:cytology / Q000196:embryology*; D002118:Calcium / Q000493:pharmacokinetics*; D005314:Embryonic and Fetal Development / Q000502:physiology; D005260:Female; D015640:Ion Channel Gating; D009046:Motor Neurons / Q000502:physiology*; D009924:Organ Culture Techniques; D010539:Permeability; D051381:Rats; D017208:Rats, Wistar; D018091:Receptors, AMPA / Q000502:physiology*
chemical_list: D018091:Receptors, AMPA; D002118:Calcium