Endothelium is required for 12-hydroperoxyeicosatetraenoic acid-induced vasoconstriction.
The pharmacological effects of 12-hydroperoxyeicosatetraenoic acid (12-HPETE) were examined using isolated canine basilar artery segments and isometric tension recording. 12-HPETE produced transient contraction of the artery segment while arachidonic acid or 12-hydroxyeicosatetraenoic acid (12-HETE) had a much lower potency. 12-HPETE-induced contraction which showed a requirement of a functional endothelium and a rapid insensitivity to re-administered 12-HPETE, was completely inhibited by the potassium channel blocker, glibenclamide. Other hydroperoxides did cross-desensitize the 12-HPETE-induced contraction, however, arachidonic acid or 12-HETE did not affect markedly. Here, we present that 12-HPETE is involved in the regulation of vascular tension via its effects on the endothelium.
Nishiyama
M
M
Division of Neurosurgery, Institute of Neurological Sciences, Tottori University School of Medicine, Nishi-machi, Yonago, Japan. makoto@biomail.ucsd.edu
Okamoto
H
H
Watanabe
T
T
Hori
T
T
Sasaki
T
T
Kirino
T
T
Shimizu
T
T
D000818:Animals; D001488:Basilar Artery / Q000187:drug effects / Q000502:physiology; D004285:Dogs; D004305:Dose-Response Relationship, Drug; D004730:Endothelium, Vascular / Q000187:drug effects* / Q000502:physiology*; D005260:Female; D005905:Glyburide / Q000494:pharmacology; D007004:Hypoglycemic Agents / Q000494:pharmacology; D007537:Isometric Contraction / Q000187:drug effects / Q000502:physiology; D015289:Leukotrienes / Q000008:administration & dosage / Q000494:pharmacology*; D008297:Male; D009131:Muscle, Smooth, Vascular / Q000187:drug effects / Q000502:physiology; D012680:Sensitivity and Specificity; D014661:Vasoconstriction / Q000187:drug effects*; D014662:Vasoconstrictor Agents / Q000008:administration & dosage / Q000494:pharmacology*
D007004:Hypoglycemic Agents; D015289:Leukotrienes; D014662:Vasoconstrictor Agents; C021201:12-HPETE; D005905:Glyburide