title: A water-soluble luminescence oxygen sensor.
abstract: We developed a water-soluble luminescent probe for dissolved oxygen. This probe is based on (Ru[dpp(SO3Na)2]3) cl2, which is a sulfonated analogue of the well-known oxygen probe (Ru[dpp]3)cl2. The compound dpp is 4,7-diphenyl-1,10-phenanthroline and dpp(SO3Na)2 is a disulfonated derivative of the same ligand. In aqueous solution in the absence of oxygen (Ru[dpp(SO3Na)2]3)cl2 displays a lifetime of 3.7 microseconds that decreases to 930 ns on equilibrium with air and 227 ns on equilibrium with 100% oxygen. The Stern-Volmer quenching constant is 11,330 M-1. This high oxygen-quenching constant means that the photoluminescence of Ru(dpp[SO3Na]2)3cl2 is 10% quenched at an oxygen concentration of 8.8 x 10(-6) M, or equilibration with 5.4 torr of oxygen. The oxygen probe dissolved in water displays minimal interactions with lipid vesicles composed of dipalmityl-L-alpha-phosphatidyl glycerol but does appear to interact with human serum albumin. The absorption maximum near 480 nm, long lifetime and large Stokes' shift allow this probe to be used with simple instrumentation based on a light-emitting diode light source, allowing low-cost oxygen sensing in aqueous solutions. To the best of our knowledge this is the first practical water-soluble oxygen sensor.
authors.affiliation: Department of Biochemistry and Molecular Biology, University of Maryland School of Medicine, Baltimore 21201, USA.
authors.forename: F N
authors.initials: FN
authors.lastname: Castellano
authors.forename: J R
authors.initials: JR
authors.lastname: Lakowicz
mesh_terms: D049449:Luminescence; D009942:Organometallic Compounds / Q000737:chemistry*; D010100:Oxygen / Q000032:analysis* / Q000737:chemistry; D010618:Phenanthrolines / Q000737:chemistry*; D012995:Solubility; D014867:Water / Q000737:chemistry
chemical_list: D009942:Organometallic Compounds; D010618:Phenanthrolines; C518776:ruthenium II tris (bathophenanthroline disulfonate); D014867:Water; D010100:Oxygen