title: An immunophilin is a component of the insect ecdysone receptor (EcR) complex.
abstract: The ecdysone receptor (EcR) complex has been identified in the prothoracic gland of Manduca sexta by specific immunoprecipitation and Western blot analyses, and includes EcR, ultraspiracle (USP) and FKBP46. The EcR complex binds ponasterone A in a dose-dependent manner with a Kd of 7.04 x 10(-9) M. Immunocytochemistry revealed that EcR, USP and FKBP46 were localized within the nucleus of the prothoracic gland cells, and suggested that the developmental expression patterns of EcR and USP changed in concert with the hemolymph ecdysteroid titer whereas that of FKBP46 did not. The composite results suggest that the hemolymph ecdysteroid titer, of which 20 hydroxyecdysone is the major component, modulates the expression of both EcR and USP in the prothoracic gland to achieve feedback regulation.
authors.affiliation: Department of Biology, University of North Carolina, Chapel Hill 27599-3280, USA.
authors.forename: Q
authors.initials: Q
authors.lastname: Song
authors.forename: E S
authors.initials: ES
authors.lastname: Alnemri
authors.forename: G
authors.initials: G
authors.lastname: Litwack
authors.forename: L I
authors.initials: LI
authors.lastname: Gilbert
mesh_terms: D000818:Animals; D000918:Antibody Specificity; D015153:Blotting, Western; D002352:Carrier Proteins / Q000032:analysis* / Q000378:metabolism; D004268:DNA-Binding Proteins / Q000032:analysis* / Q000378:metabolism; D029721:Drosophila Proteins; D004440:Ecdysone / Q000378:metabolism; D004441:Ecdysterone / Q000031:analogs & derivatives / Q000378:metabolism; D005246:Feedback; D006360:Heat-Shock Proteins / Q000032:analysis* / Q000378:metabolism; D006458:Hemolymph / Q000502:physiology; D018402:Manduca / Q000502:physiology*; D011987:Receptors, Steroid / Q000737:chemistry* / Q000378:metabolism*; D022021:Tacrolimus Binding Proteins; D013909:Thorax; D014157:Transcription Factors / Q000032:analysis* / Q000378:metabolism
chemical_list: D002352:Carrier Proteins; D004268:DNA-Binding Proteins; D029721:Drosophila Proteins; D006360:Heat-Shock Proteins; D011987:Receptors, Steroid; D014157:Transcription Factors; C066030:USP protein, Drosophila; C055469:ecdysone receptor; D004440:Ecdysone; D004441:Ecdysterone; C008999:ponasterone A; D022021:Tacrolimus Binding Proteins