title: Evaluation of 31P metabolite differences in human cerebral gray and white matter.
abstract: 31P NMR is commonly used to study brain energetics in health and disease. Due to sensitivity constraints, the NMR measurements are typically made in volumes that do not contain pure gray or white matter. For accurate evaluation of abnormalities in brain metabolite levels, it is necessary to consider the differences in normal levels of 31P metabolites in gray and white matter. In this study, voxels from a three-dimensional spectroscopic image acquisition were analyzed for their dependence on tissue type to assess differences in metabolite levels between gray and white matter. Specifically, gray matter was found to have significantly higher ratios of phosphocreatine (PCr) to gamma-ATP and PCr to the total 31P metabolite signal, whereas pH and the ratio of PCr to inorganic phosphate (Pi) were found to differ insignificantly between gray and white matter. Thus, tissue type can be an important factor to consider for alterations in bioenergetics by 31P NMR spectroscopic studies of the brain.
authors.affiliation: Department of Medicine, Center for Nuclear Imaging Research, University of Alabama at Birmingham, USA.
authors.forename: G F
authors.initials: GF
authors.lastname: Mason
authors.forename: W J
authors.initials: WJ
authors.lastname: Chu
authors.forename: J T
authors.initials: JT
authors.lastname: Vaughan
authors.forename: S L
authors.initials: SL
authors.lastname: Ponder
authors.forename: D B
authors.initials: DB
authors.lastname: Twieg
authors.forename: D
authors.initials: D
authors.lastname: Adams
authors.forename: H P
authors.initials: HP
authors.lastname: Hetherington
mesh_terms: D000255:Adenosine Triphosphate / Q000032:analysis; D000328:Adult; D000465:Algorithms; D001921:Brain / Q000033:anatomy & histology / Q000378:metabolism*; D001927:Brain Diseases / Q000378:metabolism / Q000473:pathology; D004734:Energy Metabolism; D005069:Evaluation Studies as Topic; D005260:Female; D005583:Fourier Analysis; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007091:Image Processing, Computer-Assisted; D008279:Magnetic Resonance Imaging; D009682:Magnetic Resonance Spectroscopy; D008297:Male; D010710:Phosphates / Q000032:analysis; D010725:Phosphocreatine / Q000032:analysis; D010758:Phosphorus / Q000032:analysis / Q000134:cerebrospinal fluid / Q000378:metabolism*; D010759:Phosphorus Isotopes; D012044:Regression Analysis
chemical_list: D010710:Phosphates; D010759:Phosphorus Isotopes; D010725:Phosphocreatine; D010758:Phosphorus; D000255:Adenosine Triphosphate