title: A randomized study of multi-day infusion of autologous peripheral blood progenitor cells.
abstract: Peripheral blood progenitor cells (PBPC) are increasingly used as the source of stem cells in both the autologous and allogeneic settings. Based on previous early non-randomized studies reporting enhanced engraftment following fractionated autologous PBPC infusion, some centers and study groups infuse PBPC over 3 days. To study the possible benefit of multiple day PBPC infusion, 60 patients receiving high-dose chemotherapy and autologous progenitor cell transplantation (ABMT) were randomized to receive their PBPC divided over 1, 2 or 3 days. Stem cells were mobilized with G-CSF 5 microg/kg for 7 days and PBPC were collected on days 5-7. Patients received daily G-CSF 5 microg/kg i.v. over 30 min beginning 4 h after the infusion of the first aliquot of PBPCs. Toxicity was similar for the 1, 2 and 3 day infusion groups. The median time to achieve 500 neutrophils/mm3 was 10, 11 and 11 days in the groups receiving PBPCs over 1, 2 or 3 days, respectively. The median time to achieve a platelet count of 20 x 10(9)/l was 11 days for the group receiving their cells as a single infusion and 12 days in the other two groups. We conclude that expanding PBPC infusion over 2 or 3 days does not enhance engraftment or reduce toxicity.
authors.affiliation: Department of Hematology and Medical Oncology, The Cleveland Clinic Foundation, OH 44195, USA.
authors.forename: H
authors.initials: H
authors.lastname: Abdel-Razeq
authors.forename: B
authors.initials: B
authors.lastname: Pohlman
authors.forename: S
authors.initials: S
authors.lastname: Andresen
authors.forename: M
authors.initials: M
authors.lastname: Kalaycio
authors.forename: B
authors.initials: B
authors.lastname: Overmoyer
authors.forename: K
authors.initials: K
authors.lastname: Wise
authors.forename: R
authors.initials: R
authors.lastname: Dannley
authors.forename: D
authors.initials: D
authors.lastname: DeMars
authors.forename: M
authors.initials: M
authors.lastname: Goormastic
authors.forename: B
authors.initials: B
authors.lastname: Bolwell
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D018952:Antigens, CD34 / Q000032:analysis; D005260:Female; D018380:Hematopoietic Stem Cell Transplantation / Q000009:adverse effects / Q000379:methods*; D006801:Humans; D008297:Male; D011446:Prospective Studies
chemical_list: D018952:Antigens, CD34