Electric fields and proliferation in a dermal wound model: cell cycle kinetics.
In a dermal wound model, consisting of human skin fibroblasts in collagen matrix, continuous sinusoidal electrical current stimulation elicited a maximum increase of [3H]thymidine relative to control at 41 mV/m amplitude, 10 Hz. In this paper we elaborate cell cycle kinetics, using the same parameters. Labeling occurred over 4-h intervals beginning at 12 to 20 h after onset of electric exposure. The results suggest a significant increase in [3H]thymidine incorporation over an 8-h period extending from 16-24 hours after stimulus initiation.
Cheng
K
K
Department of Rehabilitation Medicine, University of Pennsylvania, Philadelphia 19104, USA.
Goldman
R J
RJ
D002453:Cell Cycle / Q000528:radiation effects*; D002455:Cell Division / Q000528:radiation effects; D002460:Cell Line; D003094:Collagen / Q000378:metabolism; D004553:Electric Conductivity; D004558:Electric Stimulation / Q000295:instrumentation; D004574:Electromagnetic Fields; D005109:Extracellular Matrix / Q000378:metabolism; D005347:Fibroblasts / Q000166:cytology / Q000528:radiation effects; D006801:Humans; D008297:Male; D008954:Models, Biological; D012867:Skin / Q000166:cytology / Q000293:injuries
D003094:Collagen