Efficient PCR-based random mutagenesis of sub-genic (100 bp) DNA fragments.
Here we describe a method for performing a PCR-driven random mutagenesis of 100 bp DNA fragments that yields mutations at a useful frequency. The method is a modification of the manganese ion substitution PCR technique, and neither creates 'hot-spots' nor favors transition mutations over transversions.
Svetlov
V
V
Department of Microbiology and Immunology, University of Tennessee, Memphis 38163, USA.
Cooper
T G
TG
D004271:DNA, Fungal / Q000235:genetics*; D005800:Genes, Fungal; D016296:Mutagenesis; D016133:Polymerase Chain Reaction
D004271:DNA, Fungal