[Simple immunoassays of pesticides based on the biotin-streptavidin system].
A simple method was developed for the preparation of a highly active streptavidin-peroxidase conjugate that enhances fourfold the sensitivity of bioassays. Using this conjugate, a quantitative assay of simazine and 2,4-dichlorophenoxyacetic acid (competitive ELISA) and a noninstrumental express method for simultaneous detection of these pesticides (dot-immunofiltration assay) were developed. The detection limit of the pesticides is 3-4 and 2 ng/ml and the duration of the assays is 1.5 h and 5 min for ELISA and dot-immunofiltration, respectively. The express method is easy and straightforward and enables the assay to be performed outside the laboratory.
Pavlova
I S
IS
Lyubavina
I A
IA
Zherdev
A V
AV
Zinchenko
A A
AA
D015084:2,4-Dichlorophenoxyacetic Acid / Q000032:analysis* / Q000302:isolation & purification; D000906:Antibodies; D001710:Biotin / Q000737:chemistry; D002851:Chromatography, High Pressure Liquid; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D005374:Filtration; D006540:Herbicides / Q000032:analysis*; D009195:Peroxidase; D012839:Simazine / Q000032:analysis* / Q000302:isolation & purification; D019809:Streptavidin / Q000737:chemistry
D000906:Antibodies; D006540:Herbicides; D015084:2,4-Dichlorophenoxyacetic Acid; D001710:Biotin; D019809:Streptavidin; D009195:Peroxidase; D012839:Simazine
Prostye immunokhimicheskie metody opredeleniya pestitsidov na osnove biotin-streptavidinovoĭ sistemy.