[Properties of extracellular products produced by group A streptococci isolated from patients with streptococcal toxic shock syndrome].
Extracellular products of group A streptococci isolated from patients with streptococcal toxic shock syndrome (STSS) were examined. The outline of the discussion of the 3 products are as follows; streptolysin O (SLO), proteinase and erythrogenic toxin. SLO and proteinase showed a relatively large amount of products more than erythrogenic toxin. SLO produced by group A streptococci isolated from the patient with STSS had an isoelectric point (pI) of 6.0 and a molecular weight of 64,000 and showed hemolytic activity in the presence of 2-mercaptoethanol (2-ME). Furthermore, the hemolytic activities of all components were inhibited by gamma-globulin and cholesterol. Proteinase had pIs of 8.7 and 8.9, and a molecular weight of 21,000. These data suggest that STSS clinical criteria probably reflects a characteristic of a large amount of products of individual S. pyogenes isolates.
Suzuki
J
J
Department of Bioorganic Chemistry, College of Environmental Health Azabu University.
Matsubuchi
S
S
Yoshihara
E
E
Kobayashi
S
S
Okamura
K
K
Okuda
T
T
Okazaki
M
M
Furuya
N
N
Uchimura
H
H
Tokunaga
T
T
Shimazaki
S
S
D001426:Bacterial Proteins / Q000032:analysis*; D010450:Endopeptidases / Q000032:analysis*; D005098:Exotoxins / Q000032:analysis*; D005110:Extracellular Space / Q000737:chemistry; D006801:Humans; D008565:Membrane Proteins; D012772:Shock, Septic / Q000382:microbiology*; D013290:Streptococcal Infections / Q000382:microbiology*; D013297:Streptococcus pyogenes / Q000378:metabolism*; D013301:Streptolysins / Q000032:analysis*
D001426:Bacterial Proteins; D005098:Exotoxins; D008565:Membrane Proteins; C117980:SpeA protein, Streptococcus pyogenes; D013301:Streptolysins; C027951:erythrogenic toxin; C025964:streptolysin O; D010450:Endopeptidases