MS-681a, b, c and d, new inhibitors of myosin light chain kinase from Myrothecium sp. KY6568. I. Characterization of producing strain and production, isolation and biological activities.
Novel compounds MS-681a, b, c and d were isolated from the culture broth of a fungal strain KY6568. The strain was identified as Myrothecium sp. from its morphological characteristics. MS-681a, b, c and d inhibited the activity of purified smooth muscle myosin light chain kinase with IC50 values of 0.11, 0.29, 0.095 and 0.26 microM, respectively. Cyclic AMP-dependent protein kinase, cyclic GMP-dependent protein kinase and protein kinase C were not inhibited at 100 microM by MS-681 compounds.
Yano
H
H
Tokyo Research Laboratories, Kyowa Hakko Kogyo Co., Ltd., Japan.
Nakanishi
S
S
Ikuina
Y
Y
Ando
K
K
Yoshida
M
M
Saitoh
Y
Y
Matsuda
Y
Y
D000621:Aminoisobutyric Acids / Q000302:isolation & purification / Q000494:pharmacology; D004791:Enzyme Inhibitors / Q000302:isolation & purification* / Q000494:pharmacology*; D006999:Hypocreales / Q000737:chemistry* / Q000145:classification; D009219:Myosin-Light-Chain Kinase / Q000037:antagonists & inhibitors* / Q000187:drug effects; D010455:Peptides / Q000302:isolation & purification / Q000494:pharmacology; D013095:Spermidine / Q000031:analogs & derivatives* / Q000302:isolation & purification / Q000494:pharmacology
D000621:Aminoisobutyric Acids; D004791:Enzyme Inhibitors; C111134:MS-681a; D010455:Peptides; D009219:Myosin-Light-Chain Kinase; D013095:Spermidine