Safety factors involved in the extraction of biologically active proteins from human immunodeficiency virus.
The disruption of the viral coat of human immunodeficiency virus by Triton X-100, a nonionic detergent, is a time-dependent process which requires incubation times of 30 min or longer. Conditions for the production of a noninfectious sample from a viral pellet that can be used to measure reverse transcriptase activity were determined.
White
E L
EL
Southern Research Institute, Birmingham, AL 35205, USA. white@sri.org
Bowdon
B J
BJ
Shannon
W M
WM
D002460:Cell Line; D005990:Glycerol; D054303:HIV Reverse Transcriptase / Q000378:metabolism*; D015497:HIV-1 / Q000201:enzymology / Q000502:physiology*; D006801:Humans; D017830:Octoxynol; D012449:Safety; D014771:Virion / Q000201:enzymology / Q000502:physiology; D014773:Virology / Q000379:methods*
D017830:Octoxynol; D054303:HIV Reverse Transcriptase; D005990:Glycerol