title: [Development of KRN7000, derived from agelasphin produced by Okinawan sponge].
abstract: New glycosphingolipids, named agelasphins, have been isolated as antitumoral compounds from an extract of a marine sponge, Agelas mauritianus. The absolute configurations of agelasphins were elucidated by the total synthesis. Various analogues of agelasphins were also synthesized and the relationship between their structures and biological activities was examined using an MLR assay. From the results, KRN7000, (2S,3S,4R)-1-O-(alpha-D- galactopyranosyl)-2-(N-hexacosanoylamino)-1,3,4-octadecanetriol , was selected as a candidate for clinical application. KRN7000 markedly stimulated lymphocytic proliferation in allogeneic MLR, and showed potent tumor growth inhibitory activities in B16-bearing mice and strongly inhibited tumor metastasis, suggesting that KRN7000 is a potent biological response modifier. These biological effects were exerted by the activation of dendritic cells by KRN7000.
authors.affiliation: Pharmaceutical Research Laboratory, Kirin Brewery Co., Ltd., Gunma, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Natori
authors.forename: K
authors.initials: K
authors.lastname: Akimoto
authors.forename: K
authors.initials: K
authors.lastname: Motoki
authors.forename: Y
authors.initials: Y
authors.lastname: Koezuka
authors.forename: T
authors.initials: T
authors.lastname: Higa
mesh_terms: D000818:Animals; D000970:Antineoplastic Agents / Q000138:chemical synthesis* / Q000494:pharmacology; D016207:Cytokines / Q000378:metabolism; D004354:Drug Screening Assays, Antitumor; D005699:Galactosylceramides / Q000138:chemical synthesis* / Q000494:pharmacology; D006028:Glycosphingolipids / Q000494:pharmacology; D007155:Immunologic Factors / Q000138:chemical synthesis* / Q000494:pharmacology; D008213:Lymphocyte Activation; D051379:Mice; D009374:Neoplasms, Experimental / Q000473:pathology; D011161:Porifera / Q000378:metabolism; D013329:Structure-Activity Relationship
chemical_list: D000970:Antineoplastic Agents; D016207:Cytokines; D005699:Galactosylceramides; D006028:Glycosphingolipids; D007155:Immunologic Factors; C103873:KRN 7000