title: Mouse Mesenchyme forkhead 2 (Mf2): expression, DNA binding and induction by sonic hedgehog during somitogenesis.
abstract: Cloning and sequencing of mouse Mf2 (mesoderm/mesenchyme forkhead 2) cDNAs revealed an open reading frame encoding a putative protein of 492 amino acids which, after in vitro translation, binds to a DNA consensus sequence. Mf2 is expressed at high levels in the ventral region of newly formed somites, in sclerotomal derivatives, in lateral plate and cephalic mesoderm and in the first and second branchial arches. Other regions of mesodermal expression include the developing tongue, meninges, nose, whiskers, kidney, genital tubercule and limb joints. In the nervous system Mf2 is transcribed in restricted regions of the mid- and forebrain. In several tissues, including the early somite, Mf2 is expressed in cell populations adjacent to regions expressing sonic hedgehog (Shh) and in explant cultures of presomitic mesoderm Mf2 is induced by Shh secreted by COS cells. These results suggest that Mf2, like other murine forkhead genes, has multiple roles in embryogenesis, possibly mediating the response of cells to signaling molecules such as SHH.
authors.affiliation: Department of Cell Biology, Vanderbilt University Medical School, Nashville, TN 37232, USA.
authors.forename: S C
authors.initials: SC
authors.lastname: Wu
authors.forename: J
authors.initials: J
authors.lastname: Grindley
authors.forename: G E
authors.initials: GE
authors.lastname: Winnier
authors.forename: L
authors.initials: L
authors.lastname: Hargett
authors.forename: B L
authors.initials: BL
authors.lastname: Hogan
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D001921:Brain / Q000196:embryology / Q000378:metabolism; D019556:COS Cells; D003001:Cloning, Molecular; D004247:DNA / Q000378:metabolism*; D018076:DNA, Complementary / Q000235:genetics; D004268:DNA-Binding Proteins / Q000235:genetics* / Q000378:metabolism; D005314:Embryonic and Fetal Development / Q000235:genetics / Q000502:physiology; D051858:Forkhead Transcription Factors; D015870:Gene Expression; D053823:Hedgehog Proteins; D008648:Mesoderm / Q000378:metabolism; D051379:Mice; D008969:Molecular Sequence Data; D009687:Nuclear Proteins / Q000235:genetics; D011506:Proteins / Q000235:genetics*; D017386:Sequence Homology, Amino Acid; D019170:Somites / Q000378:metabolism*; D015534:Trans-Activators; D014157:Transcription Factors / Q000235:genetics
chemical_list: D018076:DNA, Complementary; D004268:DNA-Binding Proteins; D051858:Forkhead Transcription Factors; C111581:Foxd2 protein, mouse; D053823:Hedgehog Proteins; D009687:Nuclear Proteins; D011506:Proteins; D015534:Trans-Activators; D014157:Transcription Factors; D004247:DNA