Molecular characterization of antigen 24, a specific immunodominant antigen family from Leishmania infantum.
Leishmania infantum immunoelectrophoretic antigen 24 (AG 24), a visceral leishmaniasis associated immunodominant antigen, has been characterized with a monospecific antiserum by combining SDS-PAGE, immunoblotting, metabolic labelling, radio-immunoprecipitation and in vitro poly A+ mRNA translation. AG 24 appeared to correspond to a multi-antigen family of 6-9 members ranging from 20 to 31 kDa and proteinic by nature with no post-translational modifications. A similar banding pattern was recognized by infection sera. AG 24 was not found exposed on the cell surface.
Couvreur
B
B
Laboratory for Protozoology, Prince Leopold Institute for Tropical Medicine, Antwerp, Belgium.
Jacquet
D
D
Bollen
A
A
Le Ray
D
D
D000818:Animals; D000953:Antigens, Protozoan / Q000032:analysis* / Q000737:chemistry / Q000235:genetics / Q000276:immunology; D004591:Electrophoresis, Polyacrylamide Gel; D019084:Fluorescent Antibody Technique, Indirect; D006801:Humans; D007106:Immune Sera / Q000276:immunology; D015151:Immunoblotting / Q000379:methods; D016056:Immunodominant Epitopes / Q000032:analysis* / Q000737:chemistry / Q000235:genetics / Q000276:immunology; D007122:Immunoelectrophoresis / Q000379:methods; D018314:Leishmania infantum / Q000276:immunology*; D007898:Leishmaniasis, Visceral / Q000175:diagnosis / Q000276:immunology; D015394:Molecular Structure; D014176:Protein Biosynthesis; D012333:RNA, Messenger / Q000235:genetics; D016053:RNA, Protozoan / Q000235:genetics; D015531:Radioimmunoprecipitation Assay
D000953:Antigens, Protozoan; D007106:Immune Sera; D016056:Immunodominant Epitopes; D012333:RNA, Messenger; D016053:RNA, Protozoan