title: Multicenter evaluation of the Paragon CZE 2000 capillary zone electrophoresis system for serum protein electrophoresis and monoclonal component typing.
abstract: Serum protein electrophoresis and typing of monoclonal components (MCs) are routine but time-consuming and technically demanding assays. We evaluated capillary electrophoresis (Paragon CZE 2000) for automation of the two assays. CZE and cellulose acetate electrophoresis gave similar data on 794 samples. Within-run and between-run CVs were < 2% for albumin and gamma-globulins and 4-7% for alpha 1-, alpha 2-, and beta-globulins. Bilirubin, hemoglobin, triglycerides, and fibrinogen were found not to interfere. No carryover by capillaries was detected. The detection limit for MC was < 0.5 g/L. MC assessment by immunosubtraction on 403 samples identified the monoclonal type in all samples with peak concentrations > 10 g/L; only 50% of MCs that could not be quantified by densitometric scan were typed.
authors.affiliation: Laboratoire d'Immunologie, Centre Hospitalier Lyon Sud, Pierre-Bénite, France.
authors.forename: J
authors.initials: J
authors.lastname: Bienvenu
authors.forename: M S
authors.initials: MS
authors.lastname: Graziani
authors.forename: F
authors.initials: F
authors.lastname: Arpin
authors.forename: H
authors.initials: H
authors.lastname: Bernon
authors.forename: C
authors.initials: C
authors.lastname: Blessum
authors.forename: C
authors.initials: C
authors.lastname: Marchetti
authors.forename: G
authors.initials: G
authors.lastname: Righetti
authors.forename: M
authors.initials: M
authors.lastname: Somenzini
authors.forename: G
authors.initials: G
authors.lastname: Verga
authors.forename: F
authors.initials: F
authors.lastname: Aguzzi
mesh_terms: D000911:Antibodies, Monoclonal; D001663:Bilirubin / Q000097:blood; D001798:Blood Proteins / Q000302:isolation & purification*; D019075:Electrophoresis, Capillary / Q000295:instrumentation* / Q000379:methods; D004588:Electrophoresis, Cellulose Acetate / Q000379:methods; D005340:Fibrinogen; D006454:Hemoglobins; D006801:Humans; D007122:Immunoelectrophoresis / Q000379:methods; D007753:Laboratories / Q000592:standards; D009391:Nephelometry and Turbidimetry / Q000379:methods; D009404:Nephrotic Syndrome / Q000097:blood; D011786:Quality Control; D012016:Reference Values; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D012709:Serum Albumin / Q000302:isolation & purification; D012712:Serum Globulins / Q000302:isolation & purification; D014280:Triglycerides / Q000097:blood; D005719:gamma-Globulins / Q000302:isolation & purification
chemical_list: D000911:Antibodies, Monoclonal; D001798:Blood Proteins; D006454:Hemoglobins; D012709:Serum Albumin; D012712:Serum Globulins; D014280:Triglycerides; D005719:gamma-Globulins; D005340:Fibrinogen; D001663:Bilirubin