title: [Recombinant proteins containing amino acid sequences of two ectatomin chains].
abstract: Artificial genes for chains A and B of ectatomin, an Ectatomma tuberculatum ant toxin, were obtained by chemical and enzymic synthesis and cloned into new plasmid vectors. Expression plasmids with the genes of hybrid proteins were constructed containing human interleukin-3 or its terminal 63-mer fragment as well as chains A and B of ectatomin, which are linked via a region containing the cleavage site of specific protease, enterokinase (hybrid proteins IL3ETOXA, IL3ETOXB, ILETOXA, and ILETOXB). Escherichia coli producer strains providing a high yield of IL3ETOXA and IL3ETOXB proteins as inclusion bodies were obtained.
authors.affiliation: Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Moskow, Russia.
authors.forename: R S
authors.initials: RS
authors.lastname: Esipov
authors.forename: A I
authors.initials: AI
authors.lastname: Gurevich
authors.forename: A L
authors.initials: AL
authors.lastname: Kaiushin
authors.forename: M D
authors.initials: MD
authors.lastname: Korosteleva
authors.forename: A I
authors.initials: AI
authors.lastname: Miroshnikov
authors.forename: L V
authors.initials: LV
authors.lastname: Shevchenko
authors.forename: K A
authors.initials: KA
authors.lastname: Pluzhnikov
authors.forename: E V
authors.initials: EV
authors.lastname: Grishin
mesh_terms: D000595:Amino Acid Sequence; D000862:Ant Venoms / Q000096:biosynthesis* / Q000737:chemistry / Q000235:genetics; D001483:Base Sequence; D004586:Electrophoresis; D004591:Electrophoresis, Polyacrylamide Gel; D004765:Enteropeptidase / Q000737:chemistry; D004926:Escherichia coli / Q000235:genetics; D005822:Genetic Vectors; D006801:Humans; D007377:Interleukin-3 / Q000235:genetics; D008969:Molecular Sequence Data; D010957:Plasmids; D016133:Polymerase Chain Reaction; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000737:chemistry / Q000235:genetics
chemical_list: D000862:Ant Venoms; D007377:Interleukin-3; D011994:Recombinant Proteins; C088426:ectatomin protein, Ectatomma tuberculatum; D004765:Enteropeptidase
vernacular_title: Rekombinantnye belki, soderzhashchie aminokislotnye posledovatel'nosti dvukh tsepeĭ éktatomina.