[Study of kinetic parameters of glucose oxidase and alpha-chymotrypsin, immobilized in polymeric matrices, using a light-addressable sensor].
Light-addressable potentiometric sensors were prepared by immobilizing glucose oxidase or alpha-chymotrypsin on the surface of a pH-sensitive electrode. Two methods of immobilization were used: incorporation into a hydrophilic matrix of bovine serum albumin and incorporation into a hydrophobic matrix of modified polyethylenimine. The glucose oxidase and alpha-chymotrypsin preparations immobilized in the polyethylenimine matrix were characterized by the Michaelis constant values of 6.0 and 3.1. mM, respectively, and by pH optimums at 6.0-7.2 and 7.0-8.0, respectively. These values are consistent with results obtained by other methods. Therefore, light-addressable potentiometric sensors can be used in combination with both hydrophilic and hydrophobic matrices. The ability to combine light-addressable potentiometric sensors with hydrophobic matrices provides an opportunity for the development of potentiometric biosensors for detecting substances poorly soluble in water.
Kondrat'eva
E G
EG
Bach Institute of Biochemistry, Russian Academy of Sciences, Moscow, Russia.
Reshetilov
A N
AN
Iaropolov
A I
AI
D015374:Biosensing Techniques; D002918:Chymotrypsin / Q000378:metabolism*; D004566:Electrodes; D004800:Enzymes, Immobilized / Q000378:metabolism*; D005949:Glucose Oxidase / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D008027:Light; D010407:Penicillium / Q000201:enzymology; D011108:Polymers
D004800:Enzymes, Immobilized; D011108:Polymers; D005949:Glucose Oxidase; D002918:Chymotrypsin
Issledovanie s pomoshch'iu svetoadresuemogo sensora kinetichskikh parametrov gliukozooksidazy i alpha-khimotripsina, immobilizovannykh v polimernye matritsy.