The degradation rates of cytoplasmic tRNA, rRNA and mRNA in rats are elevated after infection with Nippostrongylus brasiliensis.
The effects of a parasitic infection with the nematode Nippostrongylus brasiliensis on the degradation rates of cytoplasmic tRNA, rRNA and mRNA in rats have been investigated by measuring the renal excretion rates of the modified RNA catabolites N6-threoninocarbonyladenosine, pseudouridine and 7-methylguanine. Between days 9 and 13 post-infection when the expulsion of N. brasiliensis is usually the most pronounced, the degradation rates of the different RNA classes were significantly higher than in the control rats (P < 0.05) by, on average, +24% (tRNA), +34% (rRNA) and +26% (mRNA). We suspect that the elevated degradation rates of RNA are related to an increased production of reactive oxygen species by the host during the expulsion of N. brasiliensis.
Topp
H
H
Forschunginstitut für Kinderernährung, Dortmund, Germany. topp@fke.uni-dortmund.de
Duden
R
R
Stephan
U
U
Schöch
G
G
D000818:Animals; D002851:Chromatography, High Pressure Liquid; D006790:Host-Parasite Interactions; D008297:Male; D009559:Nippostrongylus / Q000502:physiology*; D012333:RNA, Messenger / Q000378:metabolism* / Q000652:urine; D012335:RNA, Ribosomal / Q000378:metabolism* / Q000652:urine; D012343:RNA, Transfer / Q000378:metabolism* / Q000652:urine; D051381:Rats; D017208:Rats, Wistar; D017382:Reactive Oxygen Species / Q000378:metabolism*; D018709:Statistics, Nonparametric; D017206:Strongylida Infections / Q000235:genetics / Q000378:metabolism* / Q000469:parasitology / Q000652:urine
D012333:RNA, Messenger; D012335:RNA, Ribosomal; D017382:Reactive Oxygen Species; D012343:RNA, Transfer