title: In vivo presence of capsular polysaccharide in coagulase-negative staphylococci of ovine origin.
abstract: Three strains of coagulase-negative staphylococci identified as Staphilococcus warnery, Staphylococcus simulans and Staphylococcus haemolyticus were examine for evidence of capsular polysaccharide, in vitro by negative staining with India ink, and in vivo by transmission electron microscopy. In vitro, unstained materials surrounded clusters or single cocci. In vivo, capsula materials were surrounding phagocytosed bacteria cells. These capsular materials were either closely or loosely bound to bacterial cells, or projecting like "pili" from the surface of the cell wall. The evidence suggests that capsular polysaccharide is a common characteristic of coagulase-negative staphylococci causing prolonged intramammary infection of sheep.
authors.forename: A R
authors.initials: AR
authors.lastname: Burriel
mesh_terms: D000818:Animals; D016667:Bacterial Capsules / Q000032:analysis*; D003030:Coagulase / Q000378:metabolism; D005260:Female; D010861:Fimbriae, Bacterial; D008413:Mastitis / Q000382:microbiology / Q000662:veterinary*; D008854:Microscopy, Electron; D016624:Negative Staining; D010587:Phagocytosis; D012756:Sheep; D012757:Sheep Diseases / Q000382:microbiology*; D013210:Staphylococcus / Q000737:chemistry / Q000201:enzymology / Q000472:pathogenicity / Q000648:ultrastructure*; D014774:Virulence
chemical_list: D003030:Coagulase