title: Diethyl pyrocarbonate (DEPC) inhibits CO2 chemosensitivity in Helix aspersa.
abstract: Central CO2 chemoreceptors in poikilothermic vertebrates may not regulate ventilation at a particular pH setpoint; central chemoreceptor responses may more accurately reflect the relative charge state (alpha) of the imidazole of histidine. We have tested the alphastat hypothesis in the terrestrial, air breathing, pulmonate snail, Helix aspersa, by chemically modifying histidine residues in the central CO2 chemoreceptor area of this animal using diethyl pyrocarbonate (DEPC). After focal application of 20 mM DEPC to the central CO2 chemoreceptor region, the pneumostome, a respiratory, CO2 responsive organ in the snail, no longer responded to hypercapnic, acidotic stimulation of the central chemoreceptor area. However, pneumostomal responses to hypoxic stimulation of the pneumostome and to focal stimulation of the central chemoreceptor area with sodium nitroprusside, a respiratory stimulant in H. aspersa, remained intact after DEPC treatment. Furthermore, DEPC treatment of the central chemoreceptor area blocked pneumostomal responses to ammonia pre-pulse treatment, which changes intracellular pH, while extracellular pH is held constant. These results resemble mammalian responses to DEPC treatment and indicate that central chemoreceptor responses in H. aspersa may originate from changes in the alpha of intracellular histidine residues.
authors.affiliation: Department of Physiology, Dartmouth Medical School, Lebanon, NH 03756, USA.
authors.forename: D C
authors.initials: DC
authors.lastname: Lu
authors.forename: J S
authors.initials: JS
authors.lastname: Erlichman
authors.forename: J C
authors.initials: JC
authors.lastname: Leiter
mesh_terms: D000643:Ammonium Chloride / Q000494:pharmacology; D000818:Animals; D002245:Carbon Dioxide / Q000502:physiology*; D002628:Chemoreceptor Cells / Q000502:physiology*; D004047:Diethyl Pyrocarbonate / Q000494:pharmacology; D006372:Helix, Snails; D006639:Histidine / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D019811:Hydroxylamine / Q000494:pharmacology; D006935:Hypercapnia / Q000503:physiopathology; D000860:Hypoxia / Q000503:physiopathology; D066298:In Vitro Techniques; D009599:Nitroprusside / Q000494:pharmacology; D012119:Respiration / Q000187:drug effects / Q000502:physiology*
chemical_list: D000643:Ammonium Chloride; D002245:Carbon Dioxide; D009599:Nitroprusside; D019811:Hydroxylamine; D006639:Histidine; D004047:Diethyl Pyrocarbonate