Magnetic resonance imaging accurately estimates LV mass in a transgenic mouse model of cardiac hypertrophy.
Transgenic mice with a dysfunctional guanylyl cyclase A gene (GCA -/-) are unable to transduce the signals from atrial naturetic peptide and develop hypertension and cardiac hypertrophy. Magnetic resonance imaging (MRI) was performed to assess cardiac hypertrophy in these animals, using wild-type siblings as controls. Anesthetized mice were studied by gated multislice, multiphase cine MRI at 1.5 T. Simpson's rule was used to estimate left ventricle (LV) mass and volumes from short-axis images. Correlation between LV mass evaluated by MRI and at necropsy was excellent, with LVnecropsy = 1.04 x LVMRI + 4.69 mg (r2 = 0.95). By MRI, GCA -/- LV mass was significantly different when compared with isogenic controls [GCA -/-, 226 +/- 43 mg (n = 14) vs. controls, 156 +/- 14 mg (n = 10); P < 0.0001]. LV volumes and ejection fraction in the two groups were not significantly different. MRI provides an accurate means for the noninvasive assessment of murine cardiac phenotype and may be useful in following the effects of genetic modification.
Franco
F
F
Department of Radiology, University of Texas Southwestern Medical Center, Dallas 75235, USA.
Dubois
S K
SK
Peshock
R M
RM
Shohet
R V
RV
D000818:Animals; D009320:Atrial Natriuretic Factor / Q000378:metabolism; D006332:Cardiomegaly / Q000201:enzymology / Q000235:genetics / Q000473:pathology*; D004195:Disease Models, Animal; D006162:Guanylate Cyclase / Q000235:genetics; D006352:Heart Ventricles / Q000473:pathology; D006973:Hypertension / Q000235:genetics; D008279:Magnetic Resonance Imaging; D051379:Mice; D008822:Mice, Transgenic; D009154:Mutation; D015398:Signal Transduction
D009320:Atrial Natriuretic Factor; D006162:Guanylate Cyclase