Can monomers of yeast enolase have enzymatic activity?
Using a combination of ultraviolet spectroscopy under pressure and stopped-flow kinetics under pressure, we have shown that the monomers of yeast enolase produced by hydrostatic pressure are inactive. K(eq), deltaV and deltaV for the dissociation/inactivation produced by hydrostatic pressure have been determined under various conditions. Removing the Mg2+ from enolase, either by adding EDTA or by preparing apoenzyme, displaces the equilibrium towards monomers and decreases both deltaV and deltaV. Loss of Mg2+ contributes to the negative deltaV for dissociation; this loss occurs, at least partially, in the transition state for dissociation. Both removal of Mg(II) and dissociation of the enzyme produce major changes in the intensity of the aromatic region of the CD spectrum. We propose that these changes in the CD spectra reflect changes in the conformations of the 'mobile loops' of enolase. The precise conformation of these, loops is necessary for binding Mg2+ (and, hence, for activity) and for maintaining subunit interactions.
Kornblatt
M J
MJ
Department of Chemistry and Biochemistry, Concordia University, Montreal, Quebec, Canada. judithk@vax2.concordia.ca
Lange
R
R
Balny
C
C
D002942:Circular Dichroism; D019281:Dimerization; D004492:Edetic Acid / Q000494:pharmacology; D006874:Hydrostatic Pressure; D007700:Kinetics; D046911:Macromolecular Substances; D008274:Magnesium / Q000378:metabolism / Q000494:pharmacology; D010751:Phosphopyruvate Hydratase / Q000737:chemistry* / Q000378:metabolism*; D011487:Protein Conformation; D012441:Saccharomyces cerevisiae / Q000201:enzymology*; D013056:Spectrophotometry, Ultraviolet
D046911:Macromolecular Substances; D004492:Edetic Acid; D010751:Phosphopyruvate Hydratase; D008274:Magnesium