title: [Urease-negative Helicobacter pylori isolates from gastrointestinal mucosa of patients with peptic ulcer].
abstract: It is well known that Helicobacter pylori produced a large amount of urease which plays an important role in the maintenance of infection and adhesion. Two thousand three hundred and thirty-one specimens of the gastrointestinal mucosa were obtained from patients with peptic ulcer to isolate H. pylori strains. Of these specimens, 1602 strains of H. pylori were isolated and 7 urease-negative H. pylori strains were found. Biological characteristics (except urease production) of urease-negative H. pylori were in accord with urease-positive reference strains (ATCC strains). Furthermore, these urease-negative strains were confirmed to be H. pylori strains by PCR. These results suggest that the emergence of urease-negative strains pose a new problem for H. pylori infection in patients with peptic ulcer.
authors.affiliation: Chemotherapy Division, Mitsubishi-Kagaku Bio-Clinical Laboratories.
authors.forename: H
authors.initials: H
authors.lastname: Muraoka
authors.forename: I
authors.initials: I
authors.lastname: Kobayashi
authors.forename: M
authors.initials: M
authors.lastname: Hasegawa
authors.forename: T
authors.initials: T
authors.lastname: Saika
authors.forename: H
authors.initials: H
authors.lastname: Toda
authors.forename: M
authors.initials: M
authors.lastname: Nishida
authors.forename: J
authors.initials: J
authors.lastname: Suzuki
authors.forename: T
authors.initials: T
authors.lastname: Mine
authors.forename: T
authors.initials: T
authors.lastname: Fujita
mesh_terms: D005753:Gastric Mucosa / Q000382:microbiology*; D016480:Helicobacter pylori / Q000201:enzymology / Q000302:isolation & purification*; D006801:Humans; D007413:Intestinal Mucosa / Q000382:microbiology*; D010437:Peptic Ulcer / Q000382:microbiology*; D014510:Urease / Q000032:analysis*
chemical_list: D014510:Urease