Identification of structural elements of subunit beta of human protein kinase CK2 participating in tight physical alpha-beta intersubunit contacts directly adjacent to a surface-oriented region.
Sites essential for tight physical intersubunit interaction in protein kinase CK2, a tetramer composed of two catalytic (alpha and/or alpha') and two regulatory subunits (beta), have been assigned to the C-terminal part of subunit beta. Mutational analysis suggests region 171-181 of beta to be one of these but this is not consistent with the observation of coprecipitation of catalytic subunits by antibodies directed specifically to this beta segment which indicates that this region is accessible to antibodies even if the beta subunit is associated with the alpha subunit. In an attempt to clarify the apparent contradiction, we have subdivided beta-(155-181)-peptide, which includes the fragment of beta and that both binds to catalytic subunits and stimulates kinase activity, into six more or less overlapping peptides with a length of 9-16 amino acid residues and performed peptide competition and a subunit binding assays. The kinase-stimulating effect of beta-(155-181)-peptide was counteracted significantly by beta-(162-175)-peptide while the others had less or no effect. In an ELISA assay with the peptides covalently bound to wells via a C10 spacer arm, binding of a occurred besides beta-(155-181)-peptide only with beta-(162-175)-peptide and beta-(165-175)-peptide. The data provide the first evidence that the contact and the stimulation competences of subunit beta represent separable functions and suggest further that a tight physical contact to catalytic subunits is located at position 162-175 consisting of a hydrophobic stretch (position 162-171) and a hydrophilic binding motif, PEY (position 172-174), with the adjoining downstream part allowing for surface exposure and antibody binding. The presence of several Pro residues within that region might relate to the structural basis for both the alpha-beta interaction and the surface orientation.
Krehan
A
A
Biochemische Zellphysiologie, Deutsches Krebsforschungszentrum, Heidelberg, Germany.
Meggio
F
F
Pipkorn
R
R
Pinna
L A
LA
Pyerin
W
W
D000595:Amino Acid Sequence; D001665:Binding Sites; D015153:Blotting, Western; D047390:Casein Kinase II; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D046911:Macromolecular Substances; D008969:Molecular Sequence Data; D010446:Peptide Fragments / Q000737:chemistry; D011487:Protein Conformation; D017346:Protein Serine-Threonine Kinases / Q000737:chemistry* / Q000302:isolation & purification / Q000378:metabolism*; D011994:Recombinant Proteins / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism
D046911:Macromolecular Substances; D010446:Peptide Fragments; D011994:Recombinant Proteins; D047390:Casein Kinase II; D017346:Protein Serine-Threonine Kinases