title: Elevated expression of PDI family proteins during differentiation of mouse F9 teratocarcinoma cells.
abstract: We investigated the expression of protein disulfide isomerase family proteins (PDI, ERp61, and ERp72) in mouse F9 teratocarcinoma cells during differentiation induced by treatment with retinoic acid and dibutyryl cAMP. Each member of this family was expressed at a constitutive level in undifferentiated F9 cells. During differentiation of F9 cells to parietal or visceral endodermal cells the protein level of all these enzymes increased, although the extent of this increase in both protein and mRNA levels varied among the enzymes. Certain proteins were found to be coimmunoprecipitated with PDI, ERp61, and ERp72 in the presence of a chemical crosslinker. Type IV collagen was significantly coprecipitated with PDI whereas laminin was equally coprecipitated with the three proteins. Furthermore, 210 kDa protein characteristically coprecipitated with ERp72. Thus, the induction of PDI family proteins during the differentiation of F9 cells and their association with different proteins may implicate specific functions of each member of this family despite the common redox activity capable of catalyzing the disulfide bond formation.
authors.affiliation: Laboratory of Pathology, Department of Basic Gerontology, National Institute for Longevity Sciences, Obu, Japan.
authors.forename: O
authors.initials: O
authors.lastname: Miyaishi
authors.forename: K
authors.initials: K
authors.lastname: Kozaki
authors.forename: K
authors.initials: K
authors.lastname: Iida
authors.forename: K
authors.initials: K
authors.lastname: Isobe
authors.forename: Y
authors.initials: Y
authors.lastname: Hashizume
authors.forename: S
authors.initials: S
authors.lastname: Saga
mesh_terms: D000818:Animals; D015152:Blotting, Northern; D015153:Blotting, Western; D003994:Bucladesine / Q000494:pharmacology; D002454:Cell Differentiation / Q000502:physiology*; D004591:Electrophoresis, Polyacrylamide Gel; D004707:Endoderm / Q000166:cytology* / Q000201:enzymology; D004790:Enzyme Induction; D005455:Fluorescent Antibody Technique; D006360:Heat-Shock Proteins / Q000096:biosynthesis*; D006801:Humans; D007535:Isomerases / Q000096:biosynthesis*; D008562:Membrane Glycoproteins / Q000096:biosynthesis*; D051379:Mice; D011233:Precipitin Tests; D019704:Protein Disulfide-Isomerases / Q000096:biosynthesis*; D013234:Stem Cells / Q000166:cytology* / Q000201:enzymology; D018243:Teratocarcinoma; D014212:Tretinoin / Q000494:pharmacology; D014407:Tumor Cells, Cultured
chemical_list: D006360:Heat-Shock Proteins; D008562:Membrane Glycoproteins; C062477:endoplasmic reticulum glycoprotein p72; D014212:Tretinoin; D003994:Bucladesine; D007535:Isomerases; C513195:Pdia3 protein, mouse; D019704:Protein Disulfide-Isomerases; C085868:PDIA3 protein, human