title: [A channel-forming peptide toxin: polytheonamide from marine sponge (Theonella swinhoei)].
abstract: A highly cytotoxic extract from marine sponge, polytheonamide B, is a linear 48-residue peptide. Alternative D- and L-forms of unusual amino acids suggest formation of beta-helix that is stable in membrane and serves for ion conducting pore. The NMR study indicated that polytheonamide B forms beta-helix in methanol/chloroform solution. Channel activity of polytheonamide B was examined using planar lipid bilayers. Ionic current appeared from pM concentration. Measurements of the reversal potentials revealed that the channel showed cation selectivity. Single channel current was recorded in symmetrical 1 M solutions. The selectivity sequence was: H+ > Cs+ > Rb+ > K+ > Na+. Single-channel I-V curve exhibited slight inward rectification. Voltage-dependent transitions between brief openings and long closures were observed. Orientation of the peptide in the membrane was fixed when the peptide was added to one side of the chamber. The asymmetric behaviors, such as single channel rectification, voltage-dependent gating and oriented incorporation into the membrane, must be correlated to the molecular structure of polytheonamide B.
authors.affiliation: National Institute for Physiological Sciences, Aichi, Japan.
authors.forename: S
authors.initials: S
authors.lastname: Oiki
authors.forename: I
authors.initials: I
authors.lastname: Muramatsu
authors.forename: S
authors.initials: S
authors.lastname: Matsunaga
authors.forename: N
authors.initials: N
authors.lastname: Fusetani
mesh_terms: D000818:Animals; D004305:Dose-Response Relationship, Drug; D047908:Intracellular Signaling Peptides and Proteins; D015640:Ion Channel Gating / Q000187:drug effects; D007941:Leukemia P388 / Q000473:pathology; D008051:Lipid Bilayers; D008387:Marine Toxins / Q000737:chemistry / Q000494:pharmacology*; D051379:Mice; D011161:Porifera; D011506:Proteins / Q000737:chemistry / Q000494:pharmacology*
chemical_list: D047908:Intracellular Signaling Peptides and Proteins; D008051:Lipid Bilayers; D008387:Marine Toxins; D011506:Proteins; C111717:polytheonamide B