Characterisation of diarrhoeagenic Escherichia coli clones by ribotyping and ERIC-PCR.
The ability of ribotyping and enterobacterial repetitive intergenic concensus-polymerase chain reaction (ERIC-PCR) to discriminate diarrhoeagenic Escherichia coli clones of 122 strains belonging to 26 distinct serotypes was evaluated. The 26 serotypes corresponded to 24 ribotypes and 25 ERIC-types. Correlation between multilocus enzyme electrophoresis, ERIC-PCR and ribotyping was c. 90% for the dominant ribotypes. Related clones such as O55:H7 and O157:H7 presented similar ribotypes and clustered together in a dendrogram, and the two divergent clonal groups of enteropathogenic E. coli (EPEC) and enterohaemorrhagic E. coli (EHEC) were included in distinct branches. The results suggest the possibility of applying these two simpler techniques as tools to identify clones of diarrhoeagenic E. coli.
Dalla-Costa
L M
LM
Departamento de Microbiologia, Instituto de Ciências Biomédicas, Universidade de São Paulo, SP, Brazil.
Irino
K
K
Rodrigues
J
J
Rivera
I N
IN
Trabulsi
L R
LR
D015373:Bacterial Typing Techniques; D016384:Consensus Sequence; D016172:DNA Fingerprinting; D004269:DNA, Bacterial / Q000032:analysis*; D003967:Diarrhea / Q000382:microbiology*; D004586:Electrophoresis / Q000379:methods; D004926:Escherichia coli / Q000145:classification* / Q000235:genetics / Q000472:pathogenicity; D005069:Evaluation Studies as Topic; D016133:Polymerase Chain Reaction / Q000379:methods*; D012091:Repetitive Sequences, Nucleic Acid; D012703:Serotyping; D014774:Virulence
D004269:DNA, Bacterial