Stra3/lefty, a retinoic acid-inducible novel member of the transforming growth factor-beta superfamily.
We report the structure, chromosomal localization and expression features of Stra3, a novel mouse gene whose expression is upregulated by retinoic acid in P19 embryonal carcinoma cells. The Stra3 cDNA sequence, which encodes a novel member of the TGF-beta superfamily, corresponds to, but extends more 3' than the recently published lefty sequence (Meno et al., 1996, Nature 381:151-155). The Stra3/lefty protein, which exhibits characteristics of secreted proteins, is synthesized as a precursor of 42 kDa and secreted after cleavage, suggesting that it may function as an intercellular signaling molecule. There are four exons in the Stra3/lefty gene and its 5' flanking region contains, among other putative regulatory elements, an unusual retinoid response element consisting of two half sites arranged as a palindrome, with an 8 base pairs spacer. We also show that Stra3/lefty is ectopically induced in the endodermal and ectodermal layers following in vivo administration of retinoic acid to gastrulating mouse embryos.
Oulad-Abdelghani
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M
Institut de Génétique et de Biologie Moléculaire et Cellulaire, CNRS/INSERM/ULP, Collège de France, Illkirch, C.U. de Strasbourg.
Chazaud
C
C
Bouillet
P
P
Mattei
M G
MG
Dollé
P
P
Chambon
P
P
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002874:Chromosome Mapping; D003001:Cloning, Molecular; D004475:Ectoderm / Q000502:physiology; D004622:Embryo, Mammalian / Q000502:physiology; D005314:Embryonic and Fetal Development; D004707:Endoderm / Q000502:physiology; D005091:Exons / Q000235:genetics; D005775:Gastrula / Q000187:drug effects; D015972:Gene Expression Regulation, Neoplastic / Q000235:genetics; D017930:Genes, Reporter / Q000235:genetics; D017403:In Situ Hybridization; D007438:Introns / Q000235:genetics; D055458:Left-Right Determination Factors; D008297:Male; D008565:Membrane Proteins / Q000737:chemistry*; D051379:Mice; D008969:Molecular Sequence Data; D010802:Phylogeny; D011498:Protein Precursors / Q000378:metabolism; D011506:Proteins / Q000737:chemistry*; D018168:Receptors, Retinoic Acid / Q000378:metabolism; D017422:Sequence Analysis, DNA; D015398:Signal Transduction / Q000502:physiology; D013737:Testis / Q000737:chemistry / Q000196:embryology; D016212:Transforming Growth Factor beta / Q000737:chemistry* / Q000235:genetics; D014212:Tretinoin / Q000494:pharmacology*; D014407:Tumor Cells, Cultured; D015854:Up-Regulation / Q000502:physiology
D055458:Left-Right Determination Factors; C111386:Lefty1 protein, mouse; D008565:Membrane Proteins; D011498:Protein Precursors; D011506:Proteins; D018168:Receptors, Retinoic Acid; D016212:Transforming Growth Factor beta; D014212:Tretinoin