title: Mouse annexin III cDNA, genetic mapping and evolution.
abstract: Mouse annexin III cDNA was characterized from I.M.A.G.E. Consortium (LLNL) expressed sequence tag clones by molecular sequencing, chromosomal mapping and systematic analysis. cDNA sequences extended the known 5' and 3' untranslated regions and confirmed the location of intron 7 with respect to the human gene. The Anx3 locus mapped to the middle of mouse chromosome 5 between Areg and Fgf5. Protein-coding regions were compared with homologous annexins to establish subfamily identity, structural conservation and divergence pattern. Annexin III exhibited low functional constraint against structural change and weak phylogenetic association with known annexins. The rapid, constant divergence of human and rodent annexins III from each other and from other annexin subfamilies was used to estimate gene separation times. Phylogenetic, phenetic and structural data suggested a possible direct or indirect separation of annexin III from XI approximately 317 million years ago.
authors.affiliation: Department of Biochemistry and Molecular Biology, Faculty of Medicine, University of Oviedo, Spain. mpff@dwarf1.quimica.uniovi.es
authors.forename: M P
authors.initials: MP
authors.lastname: Fernández
authors.forename: N G
authors.initials: NG
authors.lastname: Copeland
authors.forename: D J
authors.initials: DJ
authors.lastname: Gilbert
authors.forename: N A
authors.initials: NA
authors.lastname: Jenkins
authors.forename: R O
authors.initials: RO
authors.lastname: Morgan
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D017318:Annexin A3 / Q000235:genetics*; D001483:Base Sequence; D002874:Chromosome Mapping; D018076:DNA, Complementary; D019143:Evolution, Molecular; D008040:Genetic Linkage; D051379:Mice; D008810:Mice, Inbred C57BL; D008969:Molecular Sequence Data; D005810:Multigene Family; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D012689:Sequence Homology, Nucleic Acid; D016324:Sequence Tagged Sites
chemical_list: D018076:DNA, Complementary; D017318:Annexin A3