Contamination of purified prostate-specific antigen preparations by kallikrein hK2.
This paper ascertained the contamination by hK2 of two types of PSA preparation, that of Sensabaugh and Blake (J. Urol., 144: 1523, 1990) and that of Deperthes et al (J. Androl., 17: 659, 1996). In the first procedure, the free forms of hK2 co-migrated with PSA during the CM-Sephadex and the Sephacryl S-200 steps. By contrast, in the second procedure a very high proportion of hK2 was separated from PSA. In two different Sensabaugh and Blake procedures, the hK2 contamination per microg. of PSA was found to be respectively 0.3 and 1.0 ng. We conclude that hK2 is a quantitatively minor contaminant of some PSA preparations. That contamination is probably of little consequence for PSA standardization but it could lead to erroneous conclusions in enzymatic studies of PSA.
Frenette
G
G
Hormonal Bioregulation Laboratory, CHUL Research Center and Laval University, Sainte-Foy, Québec, Canada.
Gervais
Y
Y
Tremblay
R R
RR
Dubé
J Y
JY
D002845:Chromatography / Q000592:standards; D006801:Humans; D007610:Kallikreins / Q000302:isolation & purification*; D017430:Prostate-Specific Antigen / Q000302:isolation & purification*; D015203:Reproducibility of Results; D012661:Semen / Q000737:chemistry
D007610:Kallikreins; D017430:Prostate-Specific Antigen