Complement-mediated cytolysis and azidothymidine are synergistic in HIV-1 suppression.
Some normal human sera contain a natural IgM antibody against gangliotetraose and GM2 which is capable of initiating complement-mediated cytolysis of HIV-1-infected cells. A potent cytolytic serum (lytic serum) harboring this antibody also caused lysis of HIV-1 virions. When lytic serum was added to a mixed culture of HIV-infected cells and naive cells at a ratio of 1:100, expansion of HIV-1 infection was postponed by 1 week. Furthermore, the co-presence of both the lytic serum and azidothymidine continued to significantly suppress infection, even after 4 weeks of cultivation.
Okada
H
H
Department of Molecular Biology, Nagoya City University School of Medicine, Nagoya, Japan.
Wu
X
X
Okada
N
N
D019380:Anti-HIV Agents / Q000494:pharmacology*; D015941:Complement Hemolytic Activity Assay; D003165:Complement System Proteins / Q000276:immunology*; D003602:Cytotoxicity, Immunologic; D004357:Drug Synergism; D015658:HIV Infections / Q000628:therapy*; D015497:HIV-1 / Q000187:drug effects; D006801:Humans; D007075:Immunoglobulin M / Q000097:blood / Q000276:immunology; D014771:Virion / Q000187:drug effects; D015215:Zidovudine / Q000494:pharmacology*
D019380:Anti-HIV Agents; D007075:Immunoglobulin M; D015215:Zidovudine; D003165:Complement System Proteins