Direct binding of low molecular weight haptens to ELISA plates.
Immobilization of low molecular weight haptens in ELISA usually involves their coupling to protein molecules or covalent binding to the solid phase. In this study we demonstrate that it is possible to directly bind the hapten p-aminophenylalanine to gamma-irradiated polystyrene microtiter plates for the detection of antibodies that stereospecifically recognize the chiral center of alpha-amino acids. Simple incubation of the hapten in aqueous buffer, without additional activation, results in a stable coating that is suited for use in ELISA.
Hofstetter
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O
Institut für Organische Chemie der Universität, Tübingen, Germany.
Hofstetter
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H
Then
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Schurig
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V
Green
B S
BS
D000596:Amino Acids / Q000737:chemistry / Q000276:immunology; D000818:Animals; D000906:Antibodies / Q000032:analysis* / Q000276:immunology; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D005720:Gamma Rays; D006241:Haptens / Q000378:metabolism*; D008968:Molecular Conformation; D008970:Molecular Weight; D010649:Phenylalanine / Q000031:analogs & derivatives* / Q000737:chemistry / Q000276:immunology / Q000378:metabolism; D011137:Polystyrenes / Q000378:metabolism / Q000528:radiation effects; D011817:Rabbits
D000596:Amino Acids; D000906:Antibodies; D006241:Haptens; D011137:Polystyrenes; C009722:4-aminophenylalanine; D010649:Phenylalanine