title: Enhancement of MHC class I-restricted peptide-specific T cell induction by a DNA prime/MVA boost vaccination regime.
abstract: Human immunodeficiency virus (HIV) vaccine candidates were previously constructed as a string of cytotoxic T lymphocyte (CTL) epitopes delivered and expressed using DNA and modified virus Ankara (MVA; an attenuated vaccinia virus) vectors. These vaccines were shown to induce interferon (IFN)-gamma-producing and cytolytic CD8+ T cells after a single vaccine administration. In the course of this work, immunization protocols were sought which would improve the levels of induced HIV-specific T cells. It was found that previous immunological exposure to MVA reduced the efficiency of subsequent priming and boosting using the same vaccine vehicle. However, a combined regime whereby the animals were first primed with the DNA vaccine and then boosted with MVA was the most potent protocol for the induction of both interferon-gamma-producing and cytolytic T cells against two CTL epitopes simultaneously. The general applicability of this novel vaccination method for induction of major histocompatibility complex class I-restricted T cells is discussed.
authors.affiliation: Molecular Immunology Group, University of Oxford, John Radcliffe Hospital, U.K.
authors.forename: T
authors.initials: T
authors.lastname: Hanke
authors.forename: T J
authors.initials: TJ
authors.lastname: Blanchard
authors.forename: J
authors.initials: J
authors.lastname: Schneider
authors.forename: C M
authors.initials: CM
authors.lastname: Hannan
authors.forename: M
authors.initials: M
authors.lastname: Becker
authors.forename: S C
authors.initials: SC
authors.lastname: Gilbert
authors.forename: A V
authors.initials: AV
authors.lastname: Hill
authors.forename: G L
authors.initials: GL
authors.lastname: Smith
authors.forename: A
authors.initials: A
authors.lastname: McMichael
mesh_terms: D000818:Animals; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology; D002478:Cells, Cultured; D004279:DNA, Viral; D000939:Epitopes; D005260:Female; D015395:Histocompatibility Antigens Class I / Q000276:immunology*; D007117:Immunization, Secondary; D007369:Interferon Inducers; D007371:Interferon-gamma / Q000096:biosynthesis; D051379:Mice; D008807:Mice, Inbred BALB C; D010455:Peptides / Q000276:immunology*; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology*; D014613:Vaccines, Attenuated; D014616:Vaccinia virus / Q000276:immunology*; D014765:Viral Vaccines
chemical_list: D004279:DNA, Viral; D000939:Epitopes; D015395:Histocompatibility Antigens Class I; D007369:Interferon Inducers; D010455:Peptides; D014613:Vaccines, Attenuated; D014765:Viral Vaccines; D007371:Interferon-gamma