title: The heparin-binding lectin from ovine placenta: purification and identification as histone H4.
abstract: The heparin-binding lectin complex from ovine placental cotyledons was purified by affinity chromatography on heparin-agarose column. It showed three protein bands, which had molecular weights of 13000, 15000 and 17000 by sodium dodecylsulfate-polyacrylamide gel electrophoresis, and the presence of DNA by agarose gel electrophoresis. The protein components of the complex were separated by reverse-phase HPLC. The minimum inhibitory concentrations of glycosaminoglycans were significantly different for the lectin complex and the separated proteins, suggesting affinity changes upon DNA binding. The haemagglutinating activity specificity allowed the characterization of the fraction with a molecular weight of 13000 as the heparin-binding lectin. This protein was identified as histone H4 by internal sequencing, thus showing that this is the histone responsible for the heparin-binding property of the complex. The accompanying proteins were tentatively identified as histones H2A and H2B.
authors.affiliation: Instituto de Química y Fisicoquímica Biológicas, (UBA-CONICET), Facultad de Farmacia y Bioquímica, Buenos Aires, Argentina.
authors.forename: A L
authors.initials: AL
authors.lastname: Ambrosio
authors.forename: M M
authors.initials: MM
authors.lastname: Iglesias
authors.forename: C
authors.initials: C
authors.lastname: Wolfenstein-Todel
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D002846:Chromatography, Affinity; D002851:Chromatography, High Pressure Liquid; D004591:Electrophoresis, Polyacrylamide Gel; D005260:Female; D006493:Heparin / Q000378:metabolism; D006657:Histones / Q000737:chemistry* / Q000302:isolation & purification / Q000378:metabolism; D037102:Lectins / Q000737:chemistry* / Q000302:isolation & purification / Q000378:metabolism; D010446:Peptide Fragments / Q000737:chemistry; D010920:Placenta / Q000737:chemistry*; D011485:Protein Binding; D012756:Sheep
chemical_list: D006657:Histones; D037102:Lectins; D010446:Peptide Fragments; C085529:heparin-binding lectin; D006493:Heparin