t(6;8), t(8;9) and t(8;13) translocations associated with stem cell myeloproliferative disorders have close or identical breakpoints in chromosome region 8p11-12.
A stem-cell myeloproliferative disorder involving T- and B-cell, and myeloid lineages, is associated with three different translocations with a breakpoint in region p11-12 of chromosome 8: t(6;8)(q27;p11), t(8;9)(p11;q33), and t(8;13)(p12;q12), respectively. Using fluorescence in situ hybridization (FISH), we have analysed blood cells from a series of five patients carrying these different translocations. We have identified cosmids from chromosome region 8p11-12 that span the breakpoint in all the cases. They are specific for the FCFR1 gene that encodes a receptor for members of the FGF family. The breakpoint was further detected by Southern and pulsed-field gel electrophoresis analyses with probes from the FGFR1 locus.
Chaffanet
M
M
Laboratoire d'Oncologie Moléculaire, U.119 INSERM, Institut de Cancérologie et d'Immunologie de Marseille, France.
Popovici
C
C
Leroux
D
D
Jacrot
M
M
Adélaïde
J
J
Dastugue
N
N
Grégoire
M J
MJ
Hagemeijer
A
A
Lafage-Pochitaloff
M
M
Birnbaum
D
D
Pébusque
M J
MJ
D000328:Adult; D000368:Aged; D002874:Chromosome Mapping; D002882:Chromosomes, Human, Pair 13; D002896:Chromosomes, Human, Pair 6; D002898:Chromosomes, Human, Pair 8; D002899:Chromosomes, Human, Pair 9; D005260:Female; D005796:Genes; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D008297:Male; D008875:Middle Aged; D009196:Myeloproliferative Disorders / Q000235:genetics* / Q000473:pathology; D020794:Receptor Protein-Tyrosine Kinases; D051496:Receptor, Fibroblast Growth Factor, Type 1; D017468:Receptors, Fibroblast Growth Factor / Q000235:genetics*; D015183:Restriction Mapping; D014178:Translocation, Genetic
D017468:Receptors, Fibroblast Growth Factor; C496348:FGFR1 protein, human; D020794:Receptor Protein-Tyrosine Kinases; D051496:Receptor, Fibroblast Growth Factor, Type 1