title: Soluble P-selectin is released from activated platelets in vivo during hemodialysis.
abstract: During hemodialysis, platelets are activated across a dialyzer. Soluble P-selectin (sP-selectin) is a form of P-selectin which is a glycoprotein relocated from secretory granules to the surfaces of platelets and endothelial cells after these cells have been physiologically activated. To investigate whether sP-selectin is useful as a marker of platelet activation during hemodialysis, we measured the plasma concentration of sP-selectin by enzyme-linked immunosorbent assay in 6 patients hemodialyzed in our institute using regenerated cellulose (RC) membranes and thereafter polysulfone membranes. Concomitantly, we also measured the plasma concentration of platelet factor 4 and beta-thromboglobulin which are released from alpha-granules of activated platelets. During hemodialysis with RC membranes, the beta-thromboglobulin level was significantly increased 15 min (p < 0.05) and the sP-selectin level 15 (p < 0.05) and 180 min (p < 0.05) after initiation of dialysis on the venous side as compared with the arterial side of the hemodialyzer. During hemodialysis with polysulfone membranes, no significant variation in plasma beta-thromboglobulin and sP-selectin levels was detected. The platelet factor 4 level increased more significantly across a dialyzer 180 min after initiation of dialysis with RC than with polysulfone membranes (p < 0.01). The changes in plasma platelet factor 4 and beta-thromboglobulin levels demonstrated that platelets are more activated during hemodialysis with RC than with polysulfone membranes. The changes in plasma sP-selectin levels during hemodialysis with RC confirm that the release of P-selectin purely from activated platelets was detected by enzyme-linked immunosorbent assay. sP-selectin may be a marker of platelet activation during hemodialysis.
authors.affiliation: Department of Medicine III, Okayama University Medical School, Japan.
authors.forename: K
authors.initials: K
authors.lastname: Kawabata
authors.forename: Y
authors.initials: Y
authors.lastname: Nagake
authors.forename: K
authors.initials: K
authors.lastname: Shikata
authors.forename: S
authors.initials: S
authors.lastname: Fukuda
authors.forename: H
authors.initials: H
authors.lastname: Nakazono
authors.forename: M
authors.initials: M
authors.lastname: Takahashi
authors.forename: H
authors.initials: H
authors.lastname: Ichikawa
authors.forename: H
authors.initials: H
authors.lastname: Makino
mesh_terms: D000328:Adult; D000368:Aged; D001792:Blood Platelets / Q000737:chemistry / Q000378:metabolism* / Q000502:physiology; D002482:Cellulose; D005260:Female; D006801:Humans; D008297:Male; D008567:Membranes, Artificial; D008875:Middle Aged; D019007:P-Selectin / Q000097:blood*; D015539:Platelet Activation; D010976:Platelet Count; D010978:Platelet Factor 4 / Q000378:metabolism; D011108:Polymers; D006435:Renal Dialysis; D012995:Solubility; D013450:Sulfones; D001620:beta-Thromboglobulin / Q000378:metabolism
chemical_list: D008567:Membranes, Artificial; D019007:P-Selectin; D011108:Polymers; D013450:Sulfones; D001620:beta-Thromboglobulin; C017662:polysulfone P 1700; D010978:Platelet Factor 4; D002482:Cellulose