A miniature CCD video camera for high-sensitivity light measurements in freely behaving animals.
We developed a miniaturized, high-sensitivity camera that can be placed in areas of difficult access in freely behaving animals for neural tissue imaging. The device consists of a charged coupled device (CCD) chip, a coherent image conduit and miniature light emitting diodes (LEDs). An amplifier circuit is constructed on the camera chip and nine wires are attached for external connections. Placement of LEDs around the image conduit perimeter provides dark-field illumination, which increases detection of cellular-related light scattering changes and doubles the depth-of-view over conventional reflectance imaging procedures. The device has been successfully used to record from several deep brain structures, including the ventral medullary surface of sleeping and waking cats. The procedure allows assessment of light scattering changes that result from neural activity or detection of vital dyes to metabolic or voltage-induced activation.
Rector
D M
DM
Department of Neurobiology and the Brain Research Institute, University of California at Los Angeles School of Medicine, 90095-1763, USA.
Poe
G R
GR
Redgrave
P
P
Harper
R M
RM
D000704:Analysis of Variance; D000818:Animals; D001921:Brain / Q000502:physiology*; D002415:Cats; D004569:Electroencephalography; D004576:Electromyography; D004585:Electrooculography; D008029:Lighting; D008526:Medulla Oblongata / Q000502:physiology*; D008904:Miniaturization; D012680:Sensitivity and Specificity; D012890:Sleep / Q000502:physiology; D014741:Video Recording / Q000295:instrumentation* / Q000379:methods; D014851:Wakefulness / Q000502:physiology