title: Effect of indomethacin on cell cycle dependent cyclic AMP fluxes in tobacco BY-2 cells.
abstract: The evolution of adenosine 3',5'-cyclic monophosphate (cAMP) levels was investigated in synchronised tobacco BY-2 cells by virtue of a method based on immunoaffinity purification and analysis on electrospray tandem mass spectrometry. A transient peak in cAMP content was observed during the S and G1 phases of the cell cycle. Application of the prostaglandin inhibiting drug indomethacin at early S phase resulted in the loss of the cAMP peak in S phase and inhibited mitotic division. This inhibition of cAMP accumulation suggests the presence of a prostaglandin-dependent adenylyl cyclase activity, analogous to animal cyclases. A potential role for cAMP during the plant cell cycle is postulated.
authors.affiliation: Dept. of Biology, Universiteit Antwerpen (UIA), Wilrijk, Belgium.
authors.forename: H
authors.initials: H
authors.lastname: Ehsan
authors.forename: J P
authors.initials: JP
authors.lastname: Reichheld
authors.forename: L
authors.initials: L
authors.lastname: Roef
authors.forename: E
authors.initials: E
authors.lastname: Witters
authors.forename: F
authors.initials: F
authors.lastname: Lardon
authors.forename: D
authors.initials: D
authors.lastname: Van Bockstaele
authors.forename: M
authors.initials: M
authors.lastname: Van Montagu
authors.forename: D
authors.initials: D
authors.lastname: Inzé
authors.forename: H
authors.initials: H
authors.lastname: Van Onckelen
mesh_terms: D016590:Aphidicolin / Q000494:pharmacology; D002453:Cell Cycle / Q000187:drug effects / Q000502:physiology*; D002460:Cell Line; D000242:Cyclic AMP / Q000378:metabolism*; D016861:Cyclooxygenase Inhibitors / Q000494:pharmacology; D016193:G1 Phase; D007213:Indomethacin / Q000494:pharmacology*; D008940:Mitotic Index / Q000187:drug effects; D010947:Plants, Toxic; D016196:S Phase; D013997:Time Factors; D014026:Nicotiana / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism
chemical_list: D016861:Cyclooxygenase Inhibitors; D016590:Aphidicolin; D000242:Cyclic AMP; D007213:Indomethacin