Inhibition of graft-versus-host reaction by treatment of immature thymocytes with eicosanoids.
A model of in vivo secondary graft-versus-host (GVH) was used to appraise the behaviour of allostimulated eicosanoid-treated thymocytes. During the first step of the experiment P1 immature parental thymocytes were preincubated in the presence of P2 allogenic mature lymphocytes and eicosanoids (either PGE2 or LTB4). During the second step, the preincubated cells were injected intravenously into P1xP2 F1 irradiated hybrids and the in vivo mixed lymphocyte reaction assessed by 3H-thymidine uptake by the cells harboured in the spleen of the host. We also investigated the production of cytotoxic cells. We observed that PGE2 and LTB4 both influence the behaviour of immature thymocytes and that, in the context of allostimulation such as GVH, they induce a state of tolerance. The data are in agreement with a model of involvement of eicosanoids in education of thymocytes within the thymic organ.
Juzan
M
M
Laboratoire d'immunologie, UMR CNRS 5540-Université Victor Segalen Bordeaux 2, France.
Guibert
F
F
Gualde
N
N
D000818:Animals; D002455:Cell Division / Q000187:drug effects / Q000276:immunology; D002478:Cells, Cultured; D018920:Coculture Techniques; D003601:Cytotoxicity Tests, Immunologic; D015232:Dinoprostone / Q000494:pharmacology; D015777:Eicosanoids / Q000494:pharmacology*; D005260:Female; D006087:Graft vs Host Reaction / Q000187:drug effects* / Q000276:immunology; D007108:Immune Tolerance / Q000187:drug effects; D007975:Leukotriene B4 / Q000494:pharmacology; D007959:Lymphocyte Culture Test, Mixed; D008214:Lymphocytes / Q000276:immunology; D051379:Mice; D013936:Thymidine / Q000378:metabolism; D013950:Thymus Gland / Q000502:physiology*
D015777:Eicosanoids; D007975:Leukotriene B4; D015232:Dinoprostone; D013936:Thymidine