Distribution of clostridial cry-like genes among Bacillus thuringiensis and Clostridium strains.
The presence of two cry-like genes first identified in Clostridium bifermentans subsp. malaysia CH18 was investigated in Clostridium species including 12 subspecies of Clostridium bifermentans, 13 strains of other members of Clostridia genus, and 13 different subspecies of Bacillus thuringiensis. Oligonucleotides designed to amplify the two toxin genes, cmb71 and cmb72, were used. We found that these genes are present in 80% of the Clostridium bifermentans strains tested and in 8% of the other Clostridium and Bacillus thuringiensis strains.
Barloy
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Unité des Bactéries Entomopathogènes, Institut Pasteur, 25 rue du Dr Roux, 75724 Paris Cedex 15, France.
Lecadet
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MM
Delécluse
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D001413:Bacillus thuringiensis / Q000235:genetics*; D001427:Bacterial Toxins / Q000235:genetics*; D003013:Clostridium / Q000235:genetics*; D017931:DNA Primers; D005798:Genes, Bacterial / Q000235:genetics*; D016133:Polymerase Chain Reaction
D001427:Bacterial Toxins; D017931:DNA Primers