title: The Drosophila gene Medea demonstrates the requirement for different classes of Smads in dpp signaling.
abstract: Signals from transforming growth factor-beta (TGF-beta) ligands are transmitted within the cell by members of the Smad family, which can be grouped into three classes based on sequence similarities. Our previous identification of both class I and II Smads functioning in a single pathway in C. elegans, raised the issue of whether the requirement for Smads derived from different classes is a general feature of TGF-beta signaling. We report here the identification of a new Drosophila class II Smad, Medea, a close homolog of the human tumor-suppressor gene DPC4. Embryos from germline clones of both Medea and Mad (a class I Smad) are ventralized, as are embryos null for the TGF-beta-like ligand decapentaplegic (dpp). Loss of Medea also blocks dpp signaling during later development, suggesting that Medea, like Mad, is universally required for dpp signaling. Furthermore, we show that the necessity for these two closely related, non-redundant Smads, is due to their different signaling properties - upon activation of the Dpp pathway, Mad is required to actively translocate Medea into the nucleus. These results provide a paradigm for, and distinguish between, the requirement for class I and II Smads in Dpp/BMP signaling.
authors.affiliation: Waksman Institute, Department of Molecular Biology, Cancer Institute of New Jersey, Rutgers University, Piscataway, NJ 08854, USA.
authors.forename: P
authors.initials: P
authors.lastname: Das
authors.forename: L L
authors.initials: LL
authors.lastname: Maduzia
authors.forename: H
authors.initials: H
authors.lastname: Wang
authors.forename: A L
authors.initials: AL
authors.lastname: Finelli
authors.forename: S H
authors.initials: SH
authors.lastname: Cho
authors.forename: M M
authors.initials: MM
authors.lastname: Smith
authors.forename: R W
authors.initials: RW
authors.lastname: Padgett
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D019521:Body Patterning / Q000235:genetics*; D002460:Cell Line; D003433:Crosses, Genetic; D004268:DNA-Binding Proteins; D004330:Drosophila / Q000196:embryology* / Q000235:genetics*; D029721:Drosophila Proteins; D004625:Embryo, Nonmammalian / Q000502:physiology*; D019143:Evolution, Molecular; D005123:Eye / Q000196:embryology / Q000648:ultrastructure; D005260:Female; D017344:Genes, Insect; D016147:Genes, Tumor Suppressor; D006801:Humans; D019476:Insect Proteins / Q000235:genetics / Q000502:physiology*; D008297:Male; D008855:Microscopy, Electron, Scanning; D008958:Models, Molecular; D008969:Molecular Sequence Data; D016296:Mutagenesis; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D011487:Protein Conformation; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid; D015398:Signal Transduction; D051901:Smad4 Protein; D015534:Trans-Activators / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism*; D014158:Transcription, Genetic; D014162:Transfection; D016212:Transforming Growth Factor beta / Q000502:physiology; D014921:Wings, Animal / Q000254:growth & development
chemical_list: D004268:DNA-Binding Proteins; D029721:Drosophila Proteins; D019476:Insect Proteins; C112504:Med protein, Drosophila; C497796:SMAD4 protein, human; D051901:Smad4 Protein; D015534:Trans-Activators; D016212:Transforming Growth Factor beta; C064196:dpp protein, Drosophila