Temporal expression of the AcMNPV lef-4 gene and subcellular localization of the protein.
Autographa californica nucleopolyhedrovirus (AcMNPV) lef-4 gene [ORF 90; Ayres et al. (1994) Virology 202, 586-605] is involved in both late and very late gene expression [Passarelli and Miller (1993) Virology 197, 704-714]. The transcriptional properties of this gene have been analyzed. It is transcribed as a single 1.6-kb mRNA and transcripts were first detected 3 h postinfection (pi). The extremities of the transcript have been mapped by primer extension and 3' RACE-PCR to positions -56 from the translation start codon and +96 downstream of the stop codon. A rabbit polyclonal antiserum has been raised against an internal polypeptide of LEF-4. A 55-kDa protein was observed by Western blot analysis from 5 h pi. LEF-4 localizes preferentially in the nucleus of infected cells and is associated with the virogenic stroma.
Durantel
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Unité de Génétique des Virus, INRA-URA CNRS 2209, Saint Christol-les-Alès, France.
Croizier
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Ravallec
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López-Ferber
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M
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D015152:Blotting, Northern; D015153:Blotting, Western / Q000379:methods; D002460:Cell Line; D002874:Chromosome Mapping; D004279:DNA, Viral; D015870:Gene Expression; D016253:Microscopy, Immunoelectron; D008969:Molecular Sequence Data; D009036:Moths / Q000821:virology; D017924:Nucleopolyhedroviruses / Q000235:genetics* / Q000378:metabolism; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger; D012367:RNA, Viral; D011817:Rabbits; D018411:Spodoptera / Q000166:cytology; D013347:Subcellular Fractions / Q000378:metabolism; D014158:Transcription, Genetic; D014764:Viral Proteins / Q000096:biosynthesis / Q000235:genetics* / Q000378:metabolism
D004279:DNA, Viral; D012333:RNA, Messenger; D012367:RNA, Viral; D014764:Viral Proteins; C084398:late expression factor 4, baculovirus