title: Effects of endogenous acetylcholine on spontaneous activity in rat dorsal cochlear nucleus slices.
abstract: We have examined the contribution of endogenous acetylcholine (ACh) release to the spontaneous firing of both regular (probably fusiform cells) and bursting neurons (probably cartwheel cells) in the dorsal cochlear nucleus (DCN) in rat brainstem slices. The muscarinic antagonists atropine, scopolamine, and tropicamide (1-2 microM) caused substantial decreases of firing rates in a majority of the neurons. Reversible acetylcholinesterase (AChE) inhibitors typically caused large transient increases in firing that decayed more slowly than responses to carbachol. The irreversible AChE inhibitor diisopropyl fluorophosphate (DFP) usually caused a sustained increase, with an initial peak followed by a gradual change to a final level higher than before DFP. Tropicamide caused large decreases in firing after DFP, confirming sustained ACh release. Both neostigmine and DFP applied after AChE inhibition by DFP sometimes elicited a transient response. We conclude that the level of sustained response to DFP is determined by the rate of endogenous ACh release, and that DFP and reversible AChE inhibitors exert an initial transient agonist effect that overlaps the initial effect of acetylcholinesterase inhibition. The slice experiments provide a model for cholinergic mechanisms in vivo, confirm that the release of endogenous ACh increases the firing rates of regular and bursting neurons in superficial DCN, and support the hypothesis that spontaneous firing of DCN neurons is sustained in part by cholinergic inputs.
authors.affiliation: Department of Otolaryngology-Head and Neck Surgery, Medical College of Ohio, Toledo, OH 43614-5807, USA.
authors.forename: K
authors.initials: K
authors.lastname: Chen
authors.forename: H J
authors.initials: HJ
authors.lastname: Waller
authors.forename: D A
authors.initials: DA
authors.lastname: Godfrey
mesh_terms: D000109:Acetylcholine / Q000378:metabolism*; D000818:Animals; D001285:Atropine / Q000494:pharmacology; D048429:Cell Size; D002800:Cholinesterase Inhibitors / Q000494:pharmacology; D017626:Cochlear Nucleus / Q000737:chemistry / Q000166:cytology / Q000378:metabolism*; D005260:Female; D007531:Isoflurophate / Q000494:pharmacology; D008297:Male; D018727:Muscarinic Antagonists / Q000494:pharmacology; D009388:Neostigmine / Q000494:pharmacology; D009474:Neurons / Q000737:chemistry / Q000187:drug effects / Q000378:metabolism; D009924:Organ Culture Techniques; D010507:Periodicity; D051381:Rats; D017207:Rats, Sprague-Dawley; D012601:Scopolamine / Q000494:pharmacology
chemical_list: D002800:Cholinesterase Inhibitors; D018727:Muscarinic Antagonists; D007531:Isoflurophate; D009388:Neostigmine; D001285:Atropine; D012601:Scopolamine; D000109:Acetylcholine