Modification of topA in Synechococcus sp. PCC 7942 resulted in mutants capable of growing under low but not high concentration of CO2.
Insertion of a cartridge encoding kanamycin resistance within an open reading frame, ORF839, in the cyanobacterium Synechococcus sp. PCC 7942 resulted in merodiploids bearing both the normal and the modified ORF839, suggesting that its gene product is essential for growth. In the absence of kanamycin the mutants were able to grow like the wild type, but in its presence the mutants grew under 0.015% CO2 in air but not under 5% CO2 in air. ORF839, identified in this study, is highly homologous to topA encoding topoisomerase I in several organisms, but it does not contain the zinc-binding motif identified in the C-terminal region of the enzyme from Escherichia coli.
Gabay
C
C
Department of Plant Sciences, Moshe Shilo Center, The Hebrew University of Jerusalem, Israel.
Lieman-Hurwitz
J
J
Hassidim
M
M
Ronen-Tarazi
M
M
Kaplan
A
A
D002245:Carbon Dioxide / Q000494:pharmacology*; D000458:Cyanobacteria / Q000235:genetics* / Q000254:growth & development; D004264:DNA Topoisomerases, Type I / Q000235:genetics*; D005798:Genes, Bacterial; D009154:Mutation
D002245:Carbon Dioxide; D004264:DNA Topoisomerases, Type I