Identification of two distinct deleted regions on chromosome 13 in prostate cancer.
Aberrations of 13q occur frequently in prostate cancer and this chromosome contains two known tumor suppressor genes, BRCA2 and Rb1. This study analysed 13q LOH, DNA ploidy, BRCA2 mutation and pRb expression in prostate cancers. In total, 13q deletions were found in 18 of 36 tumors but did not correlate with histological grade, stage or DNA ploidy. Two smallest regions of overlapping deletions were defined: one flanked by D13S218 and D13S153; the other flanked by D13S31 and D13S137. BRCA2 was less frequently deleted whereas Rb1 did have a high frequency of deletion. None of the two genes was located in any of these two regions. Furthermore, BRCA2 mutation was not found in the five tumors where deletions had involved the BRCA2 locus. Neither did the Rb1 deletion correlate with absent pRb expression. In addition, tetraploidy was found in 14 out of 25 tumors analysed and correlated with aberrant pRb expression. Our results indicate that 13q deletion is an early non-random event. Tumor suppressor genes other than BRCA2 or Rb1 may be the target of 13q deletions. Aberrant pRb expression may not reflect the two-hit Rb1 inactivation but may be involved in the tetraploidization of prostate cancer cells.
Li
C
C
Department of Urology, Karolinska Hospital, Stockholm, Sweden.
Larsson
C
C
Futreal
A
A
Lancaster
J
J
Phelan
C
C
Aspenblad
U
U
Sundelin
B
B
Liu
Y
Y
Ekman
P
P
Auer
G
G
Bergerheim
U S
US
D000368:Aged; D002882:Chromosomes, Human, Pair 13 / Q000235:genetics*; D017353:Gene Deletion; D016161:Genes, Retinoblastoma / Q000235:genetics; D016147:Genes, Tumor Suppressor / Q000235:genetics*; D006801:Humans; D019656:Loss of Heterozygosity; D008297:Male; D008875:Middle Aged; D011003:Ploidies; D011471:Prostatic Neoplasms / Q000235:genetics*; D016160:Retinoblastoma Protein / Q000378:metabolism
D016160:Retinoblastoma Protein