Localization of a tumour-suppressor gene associated with human oral cancer on 7q31.1.
To search for the existence of a tumour-suppressor gene (TSG) associated with oral squamous cell carcinoma (SCC), PCR analysis of microsatellite polymorphisms corresponding to 14 loci which map to chromosome 7q21.3-qter was performed to screen 35 patients with oral SCC for loss of heterozygosity (LOH). LOH was observed in at least one of the loci in 19 of 34 (55.9%) informative cases. Among the loci tested, frequent LOH was restricted at D7S522 on chromosome 7q31.1, which was measured within 1 cM. Furthermore, we detected microsatellite instability (MI) in 11 of 35 (31.4%) cases tested. Our observations indicate that alterations of chromosome 7q are associated with oral SCC tumorigenesis and that 7q31.1 might harbour at least one putative TSG.
Wang
X L
XL
Department of Oral Surgery, Chiba University School of Medicine, Japan. wangx-cib@umin.ac.jp
Uzawa
K
K
Miyakawa
A
A
Shiiba
M
M
Watanabe
T
T
Sato
T
T
Miya
T
T
Yokoe
H
H
Tanzawa
H
H
D002294:Carcinoma, Squamous Cell / Q000235:genetics*; D002897:Chromosomes, Human, Pair 7; D004273:DNA, Neoplasm / Q000235:genetics; D016147:Genes, Tumor Suppressor; D006801:Humans; D019656:Loss of Heterozygosity; D018895:Microsatellite Repeats; D009062:Mouth Neoplasms / Q000235:genetics*
D004273:DNA, Neoplasm