Detection of Escherichia coli O157:H7 in the beef marketed in Malaysia.
Twelve strains of Escherichia coli O157:H7 were isolated from 9 of 25 beef samples purchased from retail stores in Malaysia. These strains produced Shiga toxin 2 with or without Shiga toxin 1 and had the eae gene and a 60-MDa plasmid. The antibiograms and the profiles of the arbitrarily primed PCR of the strains were diverse, suggesting that the strains may have originated from diverse sources.
Radu
S
S
Department of Biotechnology, Faculty of Food Science and Biotechnology, University Putra Malaysia, Serdang, Malaysia.
Abdul Mutalib
S
S
Rusul
G
G
Ahmad
Z
Z
Morigaki
T
T
Asai
N
N
Kim
Y B
YB
Okuda
J
J
Nishibuchi
M
M
D000818:Animals; D002417:Cattle; D019453:Escherichia coli O157 / Q000302:isolation & purification* / Q000472:pathogenicity; D008460:Meat / Q000382:microbiology*; D016133:Polymerase Chain Reaction; D014774:Virulence