title: Isolation of ergosterol peroxide and its reversion to ergosterol in the pathogenic fungus Sporothrix schenckii.
abstract: Ergosterol peroxide, a presumed product of the H2O2-dependent enzymatic oxidation of ergosterol, has been isolated from yeast forms of the pathogenic fungus Sporothrix schenckii. The substance, which may have a role in fungal virulence, has been characterized mainly using spectroscopic methods (1H and 13C nuclear magnetic resonance and high resolution mass spectra). The purified compound showed a molecular formula of C28H44O3, displaying characteristic features of epidioxy sterols and was reverted to ergosterol when submitted to S. schenckii enzymatic extract.
authors.affiliation: Departamento de Microbiologia e Parasitologia, Universidade Federal Fluminense, Niterói, RJ.
authors.forename: D B
authors.initials: DB
authors.lastname: Sgarbi
authors.forename: A J
authors.initials: AJ
authors.lastname: da Silva
authors.forename: I Z
authors.initials: IZ
authors.lastname: Carlos
authors.forename: C L
authors.initials: CL
authors.lastname: Silva
authors.forename: J
authors.initials: J
authors.lastname: Angluster
authors.forename: C S
authors.initials: CS
authors.lastname: Alviano
mesh_terms: D000970:Antineoplastic Agents / Q000302:isolation & purification / Q000378:metabolism*; D002855:Chromatography, Thin Layer; D004875:Ergosterol / Q000031:analogs & derivatives* / Q000302:isolation & purification / Q000378:metabolism*; D009682:Magnetic Resonance Spectroscopy; D013175:Sporothrix / Q000737:chemistry*
chemical_list: D000970:Antineoplastic Agents; C036071:ergosterol-5,8-peroxide; D004875:Ergosterol