Mechanism of photosensitization of glioblastoma and neuroblastoma cells by merocyanine 540: a lipid peroxidation study.
Mechanism of merocyanine 540 (MC540) mediated photosensitization in glioblastoma (U-87MG) and neuroblastoma (Neuro 2a) cells was investigated. Photoinduced lipid peroxidation was measured in the presence of mechanistic probes-deuterium oxide (D2O), sodium azide, superoxide dismutase (SOD), mannitol and sodium benzoate. In both the types of cells, the photoinduced lipid peroxidation was enhanced in D2O whereas it showed inhibition in the presence of sodium azide. SOD also inhibited the lipid peroxidation while sodium benzoate and mannitol had no effect. These results suggest that photosensitization of U-87MG and Neuro 2a cells by MC 540 involves both type I (free radical mediated) and type II (singlet oxygen mediated) mechanisms.
Sharma
M
M
Department of Biophysics, National Institute of Mental Health and Neuro Sciences, Bangalore, India.
Joshi
P G
PG
Joshi
N B
NB
D000818:Animals; D004354:Drug Screening Assays, Antitumor; D005909:Glioblastoma / Q000188:drug therapy*; D006801:Humans; D015227:Lipid Peroxidation / Q000187:drug effects*; D051379:Mice; D009447:Neuroblastoma / Q000188:drug therapy*; D017319:Photosensitizing Agents / Q000627:therapeutic use*; D011744:Pyrimidinones / Q000627:therapeutic use*; D014407:Tumor Cells, Cultured
D017319:Photosensitizing Agents; D011744:Pyrimidinones; C003954:merocyanine dye