Increase of foreign gene expression in monocot and dicot cells by an intron in the 5' untranslated region of a soybean phosphoenolpyruvate carboxylase gene.
A genomic clone containing part of the coding region and upstream sequences of a phosphoenolpyruvate carboxylase (PEPC) gene was isolated from a soybean genomic library. The first intron of this gene is located in the 5' untranslated region. This intron-spanning fragment is capable of increasing GUS activity in plant cells.
Kato
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Mitsui Plant Biotechnology Research Institute, TCI-A1, Ibaraki, Japan.
Itoh
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Whittier
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RF
Shibata
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D000595:Amino Acid Sequence; D001483:Base Sequence; D002460:Cell Line; D018744:DNA, Plant; D015870:Gene Expression; D017343:Genes, Plant; D017930:Genes, Reporter; D005966:Glucuronidase / Q000235:genetics / Q000378:metabolism; D007438:Introns; D008969:Molecular Sequence Data; D010730:Phosphoenolpyruvate Carboxylase / Q000096:biosynthesis / Q000235:genetics*; D014176:Protein Biosynthesis; D013025:Glycine max / Q000201:enzymology* / Q000235:genetics
D018744:DNA, Plant; D005966:Glucuronidase; D010730:Phosphoenolpyruvate Carboxylase