A mimic as internal standard to monitor PCR analysis of food-borne pathogens.
This report describes a method for the construction of a mimic molecule for monitoring PCR. A blue cheese homogenate spiked with Yersinia enterocolitica was used to demonstrate how false-negative PCR results may be avoided using the mimic. Further, the possibility of estimating bacterial numbers using the mimic was illustrated by varying the target concentration.
Thisted Lambertz
S
S
National Food Administration, Uppsala, Sweden. sula@slv.se
Ballagi-Pordány
A
A
Lindqvist
R
R
D001431:Bacteriological Techniques; D002611:Cheese / Q000382:microbiology; D004269:DNA, Bacterial / Q000032:analysis*; D004587:Electrophoresis, Agar Gel; D004996:Ethidium; D005188:False Negative Reactions; D005516:Food Microbiology; D016133:Polymerase Chain Reaction / Q000592:standards*; D012015:Reference Standards; D015008:Yersinia enterocolitica / Q000235:genetics*
D004269:DNA, Bacterial; D004996:Ethidium