[Spectral properties of bisbenzimidazole dyes upon interaction with DNA].
Absorption and fluorescence spectra of a series of bisbenzimidazole dyes were studied in the presence of various concentrations of DNA and in diverse media. Variations in spectral properties of the compounds under study were found to depend on the measurement conditions. The bases for selecting the optimal composition of the media for DNA detection were presented. The modes of substrate-ligand binding and the dynamics of the alterations of fluorescent properties of structurally varying dyes upon their interaction with DNA were considered.
Sibirtsev
V S
VS
Central Research X-Ray and Radiological Institute, Ministry of Public Health and Medical Industry of Russia, St. Petersburg, Russia.
Garabadzhiu
A V
AV
Ivanov
S D
SD
D000818:Animals; D001562:Benzimidazoles / Q000737:chemistry*; D001665:Binding Sites; D002021:Buffers; D002417:Cattle; D004247:DNA / Q000737:chemistry*; D005456:Fluorescent Dyes / Q000737:chemistry*; D008024:Ligands; D017830:Octoxynol; D013050:Spectrometry, Fluorescence; D013056:Spectrophotometry, Ultraviolet; D013950:Thymus Gland
D001562:Benzimidazoles; D002021:Buffers; D005456:Fluorescent Dyes; D008024:Ligands; D017830:Octoxynol; D004247:DNA
Spektral'nye svoĭstva krasiteleĭ bisbenzimidazol'nogo riada pri vzaimodeĭstvii s DNK.