A method for analysis of cell to cell transmission of Theileria sergenti piroplasms.
We tried to develop an experimental system for quantifying piroplasm transmission in Theileria sergenti infection using a SCID mouse model, of which red blood cells (RBC) had been substituted with bovine (Bo). A mouse hybridoma cell producing antibody against Holstein RBC was established for distinguishing Holstein RBC from Japanese Black RBC by a immuno-staining method. T. sergenti piroplasms in RBC were stained by 4',6-diamidino-2-phenylindole. With the aid of these techniques the piroplasm transmission from one to another race of Bo-RBCs could successfully be quantified in SCID mice.
Terada
Y
Y
Department of Grazing Animal Production, National Grassland Research Institute, Nishinasuno, Tochigi, Japan.
Tsuji
M
M
Nakamura
Y
Y
Hagiwara
K
K
Takahashi
K
K
Ishihara
C
C
D000818:Animals; D000911:Antibodies, Monoclonal; D001803:Blood Transfusion; D002417:Cattle; D004912:Erythrocytes / Q000276:immunology / Q000469:parasitology*; D005260:Female; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D016513:Mice, SCID; D016794:Theileria / Q000472:pathogenicity / Q000502:physiology*; D013801:Theileriasis / Q000635:transmission*
D000911:Antibodies, Monoclonal