Natural ligand of mouse CD1d1: cellular glycosylphosphatidylinositol.
Mouse CD1d1, a member of the CD1 family of evolutionarily conserved major histocompatibility antigen-like molecules, controls the differentiation and function of a T lymphocyte subset, NK1+ natural T cells, proposed to regulate immune responses. The CD1d1 crystal structure revealed a large hydrophobic binding site occupied by a ligand of unknown chemical nature. Mass spectrometry and metabolic radiolabeling were used to identify cellular glycosylphosphatidylinositol as a major natural ligand of CD1d1. CD1d1 bound glycosylphosphatidylinositol through its phosphatidylinositol aspect with high affinity. Glycosylphosphatidylinositol or another glycolipid could be a candidate natural ligand for CD1d1-restricted T cells.
Joyce
S
S
Department of Microbiology and Immunology, The Pennsylvania State University College of Medicine, Milton S. Hershey Medical Center, Hershey, PA 17033-0850, USA. sjoyce@bcmic.hmc.psu.edu
Woods
A S
AS
Yewdell
J W
JW
Bennink
J R
JR
De Silva
A D
AD
Boesteanu
A
A
Balk
S P
SP
Cotter
R J
RJ
Brutkiewicz
R R
RR
D000818:Animals; D018949:Antigens, CD1 / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism*; D001665:Binding Sites; D017261:Glycosylphosphatidylinositols / Q000737:chemistry / Q000378:metabolism*; D008024:Ligands; D013058:Mass Spectrometry; D051379:Mice; D012995:Solubility; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D016176:T-Lymphocyte Subsets / Q000276:immunology
D018949:Antigens, CD1; D017261:Glycosylphosphatidylinositols; D008024:Ligands