title: Transcript levels of aquaporin 1 and carbonic anhydrase IV as predictive indicators for prognosis of renal cell carcinoma patients after nephrectomy.
abstract: Since failure of differentiation has been suggested to be involved in the neoplastic process and progression of tumors, we evaluated whether the transcript levels of differentiation markers of proximal renal tubular cells, from which renal cell carcinoma (RCC) arises, could be used as prognostic markers. We used Northern blot analysis to study the expression of aquaporin 1 (aqp1) and carbonic anhydrase IV (ca4) genes in 66 paired samples of primary RCC and non-tumorous kidney tissues. Poor differentiation of tumor cells and non-clear cell-subtype RCC were significantly associated with low levels of aqp1 transcripts. When patients were divided into 2 groups according to level of aqpI transcript in RCC, a low level of aqp1 was significantly associated with unfavorable outcome. Among 18 patients with metastatic RCC and 40 patients with moderately differentiated RCC, those with RCC expressing low levels of aqpl mRNA demonstrated poorer survival than those with RCC expressing relatively high levels of aqp1. Similarly, decreased expression of ca4 mRNA in RCC was associated with poor survival. On multivariate analysis, transcript levels of aqpI and stage of the tumor were the independent factors predicting disease-specific survival. Transcript levels of aqp1 may serve as a new molecular prognostic marker in patients with RCC following nephrectomy.
authors.affiliation: Department of Clinical Molecular Biology, Faculty of Medicine, Kyoto University, Rakuwakai Otowa Hospital, Japan.
authors.forename: J
authors.initials: J
authors.lastname: Takenawa
authors.forename: Y
authors.initials: Y
authors.lastname: Kaneko
authors.forename: M
authors.initials: M
authors.lastname: Kishishita
authors.forename: H
authors.initials: H
authors.lastname: Higashitsuji
authors.forename: H
authors.initials: H
authors.lastname: Nishiyama
authors.forename: T
authors.initials: T
authors.lastname: Terachi
authors.forename: Y
authors.initials: Y
authors.lastname: Arai
authors.forename: O
authors.initials: O
authors.lastname: Yoshida
authors.forename: M
authors.initials: M
authors.lastname: Fukumoto
authors.forename: J
authors.initials: J
authors.lastname: Fujita
mesh_terms: D051398:Aquaporin 1; D020346:Aquaporins; D001789:Blood Group Antigens; D002256:Carbonic Anhydrases / Q000235:genetics*; D002292:Carcinoma, Renal Cell / Q000175:diagnosis* / Q000235:genetics* / Q000473:pathology; D005260:Female; D005972:Glutaminase / Q000235:genetics; D006801:Humans; D007473:Ion Channels / Q000235:genetics*; D007680:Kidney Neoplasms / Q000175:diagnosis* / Q000235:genetics* / Q000473:pathology; D008297:Male; D015999:Multivariate Analysis; D009392:Nephrectomy; D011379:Prognosis; D012333:RNA, Messenger / Q000235:genetics; D012334:RNA, Neoplasm / Q000235:genetics
chemical_list: C496020:AQP1 protein, human; D020346:Aquaporins; D001789:Blood Group Antigens; D007473:Ion Channels; D012333:RNA, Messenger; D012334:RNA, Neoplasm; D051398:Aquaporin 1; D005972:Glutaminase; D002256:Carbonic Anhydrases