Strategies for selection of antibodies by phage display.
Phage antibody-display is rapidly maturing into a very effective tool for antibody generation. The recent development of large primary antibody libraries enables selection of antibodies against most targets in under two weeks and many of these antibodies have relatively high (nanomolar) affinities. Successful strategies have also been developed to affinity mature these antibodies into the picomolar range if required.
Griffiths
A D
AD
MRC Laboratory of Molecular Biology, Cambridge, UK. griff@mrc-lmb.cam.ac.uk
Duncan
A R
AR
D000818:Animals; D000906:Antibodies / Q000235:genetics* / Q000276:immunology; D000915:Antibody Affinity; D001435:Bacteriophages / Q000235:genetics*; D001709:Biotechnology / Q000379:methods*; D015723:Gene Library; D005822:Genetic Vectors; D006801:Humans; D007114:Immunization; D007135:Immunoglobulin Variable Region / Q000235:genetics
D000906:Antibodies; D007135:Immunoglobulin Variable Region