Study of the interaction of DNA with cisplatin and other Pd(II) and Pt(II) complexes by atomic force microscopy.
Modifications in the structure of a 260 bp DNA (hlyM) fragment from Escherichia coli caused by interaction with Pd(II) and Pt(II) complexes were studied. Cisplatin and transplatin [cis- and trans-PtCl2(NH3)2 respectively], Pt2Cl2(Spym)4 (Spym = 2-mercaptopyrimidine anion), Pd-famotidine and Pt-famotidine were incubated with DNA for 24 h at 37 degrees C and then observed with an atomic force microscope. Atomic force microscopy (AFM) provides the opportunity for nanometer resolution in research on the interaction between nucleic acids and metal complexes. The complexes induced noticeable changes in DNA topography according to their different characteristics and structure. In the case of cisplatin a shortening in DNA strands was observed. Transplatin and Pt2Cl2(Spym)4 caused shortening and compaction, whilst an aggregation of two strands was observed for the Pt-famotidine compound but not for the Pd-famotidine compound or the metal-free famotidine.
Onoa
G B
GB
Departament de Química Inorgànica, Universitat de Barcelona, Diagonal 647, 08028-Barcelona, Spain.
Cervantes
G
G
Moreno
V
V
Prieto
M J
MJ
D000970:Antineoplastic Agents / Q000494:pharmacology; D001426:Bacterial Proteins / Q000235:genetics; D002945:Cisplatin / Q000494:pharmacology*; D004269:DNA, Bacterial / Q000737:chemistry / Q000187:drug effects* / Q000648:ultrastructure; D004926:Escherichia coli / Q000187:drug effects / Q000235:genetics / Q000378:metabolism; D029968:Escherichia coli Proteins; D015738:Famotidine / Q000494:pharmacology; D006460:Hemolysin Proteins / Q000235:genetics; D018625:Microscopy, Atomic Force; D009942:Organometallic Compounds / Q000494:pharmacology; D010165:Palladium / Q000494:pharmacology; D017671:Platinum Compounds / Q000494:pharmacology
D000970:Antineoplastic Agents; D001426:Bacterial Proteins; D004269:DNA, Bacterial; D029968:Escherichia coli Proteins; D006460:Hemolysin Proteins; C036507:Hlya protein, E coli; D009942:Organometallic Compounds; D017671:Platinum Compounds; C034697:transplatin; D015738:Famotidine; D010165:Palladium; D002945:Cisplatin