Accurate and precise measurement of heteronuclear long-range couplings by a gradient-enhanced two-dimensional multiple-bond correlation experiment.
We propose a phase-sensitive gradient-enhanced two-dimensional heteronuclear multiple-bond correlation (psge-2D HMBC) experiment for speedy, accurate, and precise measurement of 2JCH and 3JCH. The experiment does not suppress one-bond correlations. Rather, the value of a desired long-range JCH is obtained from the pertinent cross-peak pattern in the HMBC spectrum, using the corresponding 1JCH correlation pattern as reference. The application of the proposed experiment is illustrated for the trisaccharide raffinose.
Sheng
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Department of Chemistry, Georgia State University, Atlanta, Georgia, 30303-3083, USA.
van Halbeek
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H
D005583:Fourier Analysis; D007091:Image Processing, Computer-Assisted; D009682:Magnetic Resonance Spectroscopy / Q000379:methods*; D015394:Molecular Structure; D011887:Raffinose / Q000737:chemistry*; D015203:Reproducibility of Results; D012984:Software
D011887:Raffinose