Analysis of hopanoids in bacteria involved in food technology and food contamination.
Hopanoids are pentacyclic triterpenoids which are believed to act as reinforcers of membranes in certain prokaryotic microorganisms. A rapid and sensitive method for their screening in bacteria was elaborated, involving extraction of nonsaponifiable lipids and the analysis by gas chromatography-mass spectrometry, selectively monitoring the ion of m/z = 191. Using the method, hopanoids were detected in strains of Acetobacter pasteurianus, but were found to be absent in lactic acid bacteria (Lactobacillus spp., Lactococcus spp.) and in food-contaminating bacteria (Salmonella spp., Listeria spp., Yersinia spp. and others).
Kuchta
T
T
Department of Microbiology and Chemistry, Food Research Institute, Bratislava, Slovakia. holcikov@mbox.bts.sk
Kubinec
R
R
Farkas
P
P
D001419:Bacteria / Q000737:chemistry*; D005516:Food Microbiology; D014315:Triterpenes / Q000032:analysis*
D014315:Triterpenes