Delivery of antigen-encoding plasmid DNA into the cytosol of macrophages by attenuated suicide Listeria monocytogenes.
Eukaryotic expression vectors can be delivered to macrophages using attenuated self-destructing Listeria monocytogenes. L. monocytogenes cells are preferentially lysed in the host cell macrophage cytosol by the production of a PactA-dependent Listeria-specific phage lysin. Efficient expression of the cloned reporter genes by the macrophages and subsequent antigen presentation were achieved after the delivery of eukaryotic expression vectors by the attenuated suicide L. monocytogenes strain. After delivery by L. monocytogenes plasmid DNAs were found to integrate into the macrophage cell's genome at a frequency of about 10(-7).
Dietrich
G
G
Lehrstuhl für Mikrobiologie, University of Würzburg, Germany.
Bubert
A
A
Gentschev
I
I
Sokolovic
Z
Z
Simm
A
A
Catic
A
A
Kaufmann
S H
SH
Hess
J
J
Szalay
A A
AA
Goebel
W
W
D000818:Animals; D001428:Bacterial Vaccines; D001433:Bacteriolysis; D001435:Bacteriophages / Q000201:enzymology / Q000235:genetics; D003587:Cytomegalovirus / Q000235:genetics; D003600:Cytosol / Q000378:metabolism; D010450:Endopeptidases / Q000235:genetics / Q000378:metabolism; D017930:Genes, Reporter; D005822:Genetic Vectors; D008089:Listeria monocytogenes / Q000235:genetics* / Q000821:virology; D008264:Macrophages / Q000378:metabolism*; D051379:Mice; D008807:Mice, Inbred BALB C; D010957:Plasmids / Q000235:genetics*; D011401:Promoter Regions, Genetic; D014407:Tumor Cells, Cultured; D014613:Vaccines, Attenuated; D019444:Vaccines, DNA
D001428:Bacterial Vaccines; D014613:Vaccines, Attenuated; D019444:Vaccines, DNA; D010450:Endopeptidases; C001143:endolysin