title: Comparative analysis of evolution in a rodent histone H2a pseudogene.
abstract: Sequences were obtained from five species of rodents that are orthologous to an H2a histone pseudogene from Mus musculus. The pseudogene is part of the cluster of replication-dependent histone genes found on Mus musculus chromosome 13. Comparative analysis of these five sequences together with the previously published sequence from M. musculus shows that this gene has likely been a pseudogene throughout the evolution of the genus Mus, while the gene from Rattus norvegicus is likely functional. Three large (> 20 bp) deletions were found among the Mus pseudogenes, a feature that is very unusual compared to surveys of processed pseudogenes. In addition, there are two single-base deletions and one 4-bp insertion among the Mus pseudogenes. The species distributions of one of the large deletions and the 4-bp insertion require either independent insertions of an identical sequence, independent deletions with identical boundaries, or a deletion followed by precise reintegration of the original sequence. The evidence favors the hypothesis of multiple deletions with identical boundaries. The "coding" regions of the Mus pseudogenes show a much reduced level of among-species variability in the 3' half of the pseudogene, compared both to the 5' half and to flanking sequences. This supports a hypothesis that the 3' end of the pseudogene is the target of frequent gene conversion by functional H2a genes.
authors.affiliation: Department of Biological Sciences, University of Cincinnati, OH 45221-0006, USA. ron.debry@uc.edu
authors.forename: R W
authors.initials: RW
authors.lastname: DeBry
mesh_terms: D000818:Animals; D001483:Base Sequence; D019143:Evolution, Molecular; D005785:Gene Conversion; D006657:Histones / Q000235:genetics*; D051379:Mice / Q000235:genetics*; D008969:Molecular Sequence Data; D016254:Mutagenesis, Insertional; D010802:Phylogeny; D011544:Pseudogenes; D051381:Rats; D017207:Rats, Sprague-Dawley; D016415:Sequence Alignment; D017384:Sequence Deletion
chemical_list: D006657:Histones