title: Dynamics of the expression of cytoskeleton components and adherens molecules by fibroblastic cells in alkali-burned and lacerated corneas.
abstract: To provide a better understanding of the role of fibroblastic cells during corneal wound-healing, we examined the expression of cytoskeleton components (i.e. smooth muscle alpha-actin (alpha-SMA), vimentin, desmin), adherens molecules (vinculin and talin) and cellular fibronectin in alkali-burned and lacerated rabbit corneas. Alkali-burned and lacerated corneas, which had healed for various periods of time (1 day to 45 days), were excised and subjected to immunohistochemical studies with monoclonal antibodies against alpha-SMA, vimentin, desmin, vinculin, talin and cellular fibronectin. Monoclonal antibody against alpha-SMA reacted with fibroblastic cells in injured corneas but did not react with keratocytes in normal corneas. Anti-desmin antibody did not react with any corneal cells in normal or injured corneas except the muscle cells in the newly-formed capillary of injured corneas. The results indicate that the fibroblastic cells in injured corneas have the characteristics of myofibroblasts. The number of myofibroblasts in granulation tissues increased and peaked within 3 weeks of injury and then declined. Electron microscopy revealed that some fibroblastic cells in the lacerated cornea which had healed for 4 weeks contained dense chromatins. In situ 3'-end labeling with terminal nucleotide transferase indicating that some of the fibroblastic cells contained nicked genomic DNA. These observations imply that apoptosis plays a role in regulating the number of myofibroblasts in the injured corneas. Antibodies against cellular fibronectin, vinculin and talin react with the fibroblastic cells in the injured corneas, but not with the keratocytes of normal corneas. Examination with transmission electron microscopy demonstrated the presence of microtendon and fibronexis associated with fibroblastic cells and the presence of stress fiber within fibroblastic cells. The results indicate that the fibroblastic cells may cause corneal wound contraction, which in turn contributes to the formation of opaque scar tissues.
authors.affiliation: Department of Pathology 1, Nippon Medical School, Tokyo, Japan.
authors.forename: M
authors.initials: M
authors.lastname: Ishizaki
authors.forename: K
authors.initials: K
authors.lastname: Wakamatsu
authors.forename: T
authors.initials: T
authors.lastname: Matsunami
authors.forename: N
authors.initials: N
authors.lastname: Yamanaka
authors.forename: T
authors.initials: T
authors.lastname: Saiga
authors.forename: Y
authors.initials: Y
authors.lastname: Shimizu
authors.forename: G
authors.initials: G
authors.lastname: Zhu
authors.forename: W W
authors.initials: WW
authors.lastname: Kao
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal; D017209:Apoptosis; D002057:Burns, Chemical / Q000378:metabolism* / Q000473:pathology; D003315:Cornea / Q000187:drug effects / Q000378:metabolism* / Q000648:ultrastructure; D065306:Corneal Injuries; D003598:Cytoskeletal Proteins / Q000378:metabolism*; D004195:Disease Models, Animal; D005126:Eye Burns / Q000139:chemically induced* / Q000473:pathology; D005131:Eye Injuries / Q000378:metabolism* / Q000473:pathology; D005260:Female; D005347:Fibroblasts / Q000378:metabolism / Q000648:ultrastructure; D005353:Fibronectins / Q000378:metabolism; D007124:Immunoenzyme Techniques; D008297:Male; D011817:Rabbits; D012972:Sodium Hydroxide; D014945:Wound Healing
chemical_list: D000911:Antibodies, Monoclonal; D003598:Cytoskeletal Proteins; D005353:Fibronectins; D012972:Sodium Hydroxide