title: Rise of cytosolic Ca2+ and activation of membrane-bound guanylyl cyclase activity in rat enterocytes by heat-stable enterotoxin of Vibrio cholerae non-01.
abstract: The cytosolic calcium level ([Ca2+]i) and the membrane-bound guanylyl cyclase activity in the isolated rat intestinal epithelial cells were investigated. Heat-stable enterotoxin of Vibrio cholerae non-01 (NAG-ST) was found to increase both the [Ca2+]i and the enzyme activity. These changes occur similarly until 5 min of incubation with NAG-ST, indicating that these changes might be involved in NAG-ST induced signal transduction in rat enterocytes.
authors.affiliation: National Institute of Cholera and Enteric Diseases, Beliaghata, Calcutta, India.
authors.forename: A G
authors.initials: AG
authors.lastname: Chaudhuri
authors.forename: J
authors.initials: J
authors.lastname: Bhattacharya
authors.forename: G B
authors.initials: GB
authors.lastname: Nair
authors.forename: T
authors.initials: T
authors.lastname: Takeda
authors.forename: M K
authors.initials: MK
authors.lastname: Chakrabarti
mesh_terms: D000818:Animals; D002118:Calcium / Q000378:metabolism*; D002478:Cells, Cultured; D004305:Dose-Response Relationship, Drug; D004768:Enterotoxins / Q000494:pharmacology*; D004789:Enzyme Activation; D004847:Epithelial Cells / Q000166:cytology / Q000201:enzymology / Q000382:microbiology*; D006162:Guanylate Cyclase / Q000378:metabolism*; D007583:Jejunum / Q000166:cytology; D051381:Rats; D013997:Time Factors; D014734:Vibrio cholerae
chemical_list: D004768:Enterotoxins; C037420:stN protein, Vibrio cholerae; D006162:Guanylate Cyclase; D002118:Calcium