Thin-layer chromatographic analysis of some beta-blocking drugs in plasma.
beta-receptor blockers--atenolol, celiprolol, metoprolol, propafenone, propranolol and talinolol--extracted from plasma, were separated by TLC method on silica gel by ascending technique. Isolation of drugs was performed by using liquid-liquid extraction after alkalization of blood plasma. Suitable conditions for separation were established using ethyl acetate-acetone-ammonia (5:4:1) as the mobile phase. The substances were identified by UV irradiation (254 nm) or by reactions with a variety of reagents. The visual limits of determination of named beta-blockers, after reaction with indicators, are between 100-300 ng; celiprolol and talinolol are the most sensitively detectable of investigated substances.
Witek
A
A
Department of Medicinal Chemistry, School of Medicine, Lublin, Poland.
Przyborowski
L
L
D000319:Adrenergic beta-Antagonists / Q000097:blood* / Q000737:chemistry; D000818:Animals; D001262:Atenolol / Q000097:blood / Q000737:chemistry; D002417:Cattle; D017272:Celiprolol / Q000097:blood / Q000737:chemistry; D002855:Chromatography, Thin Layer / Q000379:methods*; D008790:Metoprolol / Q000097:blood / Q000737:chemistry; D011405:Propafenone / Q000097:blood / Q000737:chemistry; D011412:Propanolamines / Q000097:blood / Q000737:chemistry; D011433:Propranolol / Q000097:blood / Q000737:chemistry
D000319:Adrenergic beta-Antagonists; D011412:Propanolamines; C011550:talinolol; D001262:Atenolol; D011405:Propafenone; D011433:Propranolol; D017272:Celiprolol; D008790:Metoprolol