Herpes simplex virus 1-mediated transfer of preproenkephalin A in rat dorsal root ganglia.
Recombinant herpes simplex virus-1 encoding the rat preproenkephalin A (HSVLatEnk1) was generated for driving the expression of preproenkephalin A-derived peptides in dorsal root ganglia of rats in vivo. Three weeks after infection via the hind footpads, quantitative RT-PCR and in situ hybridization experiments showed a strong expression of preproenkephalin A mRNA in lumbar dorsal root ganglia. In addition, a 40-160% increase in radioimmunoassayable Met-enkephalin-like material concentrations was found in the dorsal spinal cord and dorsal root ganglia, respectively, at the lumbar level in HSVLatEnk1-infected rats as compared with animals infected with beta-galactosidase-encoding recombinant herpes simplex virus-1 or control rats. These data demonstrate the efficacy of the preproenkephalin A encoding vector and suggest that it should help in elucidating the role of Met-enkephalin-containing primary afferent fibers in pain transmission and/or control.
Antunes Bras
J M
JM
INSERM U 288, NeuroPsychoPharmacologie Moléculaire, Cellulaire et Fonctionnelle, Faculté de Médecine Pitié-Salpêtrière, Paris, France.
Epstein
A L
AL
Bourgoin
S
S
Hamon
M
M
Cesselin
F
F
Pohl
M
M
D000818:Animals; D004745:Enkephalins / Q000235:genetics*; D005727:Ganglia, Spinal; D015967:Gene Expression Regulation, Viral; D018014:Gene Transfer Techniques; D017930:Genes, Reporter; D018259:Herpesvirus 1, Human; D006801:Humans; D008297:Male; D009447:Neuroblastoma; D010146:Pain / Q000503:physiopathology; D011401:Promoter Regions, Genetic; D011498:Protein Precursors / Q000235:genetics*; D012333:RNA, Messenger / Q000032:analysis; D051381:Rats; D017207:Rats, Sprague-Dawley; D011994:Recombinant Proteins; D014407:Tumor Cells, Cultured; D001616:beta-Galactosidase / Q000235:genetics
D004745:Enkephalins; D011498:Protein Precursors; D012333:RNA, Messenger; D011994:Recombinant Proteins; C033660:preproenkephalin; D001616:beta-Galactosidase