title: Integrative promoter cloning plasmid vectors for Rhizobium meliloti.
abstract: A set of integrative 'promoter probe' plasmids were constructed for both translational and transcriptional fusions. The vectors are based on the broad host range, low copy number plasmid pRK290 (IncPl) in which the attachment site of Rhizobium phage 16-3 and the lacZ gene of Escherichia coli were combined. The vectors integrate into the chromosome of Rhizobium meliloti, providing also the advantages of the single copy promoter probe cassettes. Thus they fulfil the prerequisite of the systems used for investigating gene regulation. The plasmids were applied for the study of the transcription regulation of the 16-3 phage. Their versatile use is also demonstrated.
authors.affiliation: Agricultural Biotechnology Center, Institute for Molecular Genetics, Gödöllö, Hungary.
authors.forename: P
authors.initials: P
authors.lastname: Elö
authors.forename: S
authors.initials: S
authors.lastname: Semsey
authors.forename: A
authors.initials: A
authors.lastname: Kereszt
authors.forename: T
authors.initials: T
authors.lastname: Nagy
authors.forename: P
authors.initials: P
authors.lastname: Papp
authors.forename: L
authors.initials: L
authors.lastname: Orosz
mesh_terms: D000595:Amino Acid Sequence; D001435:Bacteriophages; D001483:Base Sequence; D003001:Cloning, Molecular; D018628:Gene Dosage; D015964:Gene Expression Regulation, Bacterial; D017930:Genes, Reporter; D005816:Genetic Complementation Test; D005822:Genetic Vectors; D008969:Molecular Sequence Data; D010957:Plasmids; D011401:Promoter Regions, Genetic / Q000235:genetics*; D014176:Protein Biosynthesis; D011993:Recombinant Fusion Proteins; D016962:Sinorhizobium meliloti / Q000235:genetics*; D014158:Transcription, Genetic; D001616:beta-Galactosidase / Q000235:genetics
chemical_list: D011993:Recombinant Fusion Proteins; D001616:beta-Galactosidase