Conversion of cysteine to formylglycine in eukaryotic sulfatases occurs by a common mechanism in the endoplasmic reticulum.
Sulfatases undergo an unusual protein modification leading to conversion of a specific cysteine residue into alpha-formylglycine. This conversion is essential for catalytic activity. In arylsulfatase A the alpha-formylglycine is generated inside the endoplasmic reticulum at a late stage of protein translocation. Using in vitro translation in the presence of transport-competent microsomes we found that arylsulfatase B is also modified in a similar way by the formylglycine-generating machinery. Modification depended on protein transport and on the correct position of the relevant cysteine. Arylsulfatase A and B did not compete for modification, as became apparent in co-expression experiments. This could argue for an association of the modification machinery with the protein translocation apparatus.
Dierks
T
T
Institut für Biochemie und Molekulare Zellbiologie, Abt. Biochemie II, Universität Göttingen, Germany. dierks@ukb2-00.uni-bc.gwdg.de
Lecca
M R
MR
Schmidt
B
B
von Figura
K
K
D000409:Alanine / Q000031:analogs & derivatives* / Q000378:metabolism; D000818:Animals; D002460:Cell Line; D002553:Cerebroside-Sulfatase / Q000096:biosynthesis / Q000235:genetics / Q000378:metabolism*; D002803:Chondro-4-Sulfatase / Q000096:biosynthesis / Q000235:genetics / Q000378:metabolism*; D006224:Cricetinae; D003545:Cysteine / Q000235:genetics / Q000378:metabolism*; D004721:Endoplasmic Reticulum / Q000378:metabolism; D005057:Eukaryotic Cells / Q000378:metabolism; D005998:Glycine / Q000031:analogs & derivatives* / Q000378:metabolism; D006801:Humans; D011499:Protein Processing, Post-Translational; D013997:Time Factors
C524739:C(alpha)-formylglycine; D002553:Cerebroside-Sulfatase; D002803:Chondro-4-Sulfatase; D003545:Cysteine; D000409:Alanine; D005998:Glycine