title: Effects of nitric oxide synthase inhibition on phospholipid fatty acid composition of brown adipose tissue.
abstract: We investigated the effects of nitric oxide synthase inhibition on the phospholipid fatty acid composition of brown adipose tissue by administering chronic N omega-nitro-L-arginine methyl ester (L-NAME) (0.01%) to rats via drinking water. L-NAME caused a significant decrease in C14:0, C16:0, C16:1 and C18:1, with a concomitant increase in C18:0 and C18:2, L-NAME treatment elicited an increase in the unsaturation index. There were no significant changes in other fatty acids including the polyunsaturated fatty acids docosahexaenoate (DHA, C22:6) and arachidonate (C20:4). Polyunsaturated fatty acid linoleate (C18:2) was increased and the arachidonate index decreased in the L-NAME-treated group, suggesting a diminished conversion of C18:2 to C20:4.
authors.affiliation: Department of Physiology I, Asahikawa Medical College, Japan. saha@asahikawa-med.ac.jp
authors.forename: S K
authors.initials: SK
authors.lastname: Saha
authors.forename: T
authors.initials: T
authors.lastname: Ohno
authors.forename: H
authors.initials: H
authors.lastname: Ohinata
authors.forename: A
authors.initials: A
authors.lastname: Kuroshima
mesh_terms: D000273:Adipose Tissue / Q000033:anatomy & histology / Q000737:chemistry / Q000187:drug effects; D002001:Adipose Tissue, Brown / Q000033:anatomy & histology / Q000737:chemistry* / Q000187:drug effects; D000818:Animals; D016718:Arachidonic Acid / Q000032:analysis; D004791:Enzyme Inhibitors / Q000494:pharmacology; D005227:Fatty Acids / Q000032:analysis*; D005231:Fatty Acids, Unsaturated / Q000032:analysis; D019787:Linoleic Acid / Q000032:analysis; D008297:Male; D019814:Myristic Acid / Q000032:analysis; D019331:NG-Nitroarginine Methyl Ester / Q000494:pharmacology; D009569:Nitric Oxide / Q000378:metabolism; D019001:Nitric Oxide Synthase / Q000037:antagonists & inhibitors* / Q000378:metabolism; D019301:Oleic Acid / Q000032:analysis; D009929:Organ Size / Q000187:drug effects; D019308:Palmitic Acid / Q000032:analysis; D010743:Phospholipids / Q000032:analysis* / Q000737:chemistry; D051381:Rats; D017208:Rats, Wistar; D013229:Stearic Acids / Q000032:analysis
chemical_list: D004791:Enzyme Inhibitors; D005227:Fatty Acids; D005231:Fatty Acids, Unsaturated; D010743:Phospholipids; D013229:Stearic Acids; D019814:Myristic Acid; D016718:Arachidonic Acid; D019301:Oleic Acid; D019308:Palmitic Acid; D009569:Nitric Oxide; C031183:stearic acid; D019787:Linoleic Acid; D019001:Nitric Oxide Synthase; D019331:NG-Nitroarginine Methyl Ester