Dependence of BSAP repressor and activator functions on BSAP concentration.
During a B cell immune response, the transcription factor BSAP maintains its activator functions but is relieved of its repressor functions. This selective targeting of BSAP activities was shown to be regulated by a concentration-dependent mechanism whereby activator motifs for BSAP had a 20-fold higher binding affinity than repressor motifs. An exchange of activator and repressor motifs, however, showed that the context of the motif, rather than the affinity, determined whether BSAP operated as an activator or repressor.
Wallin
J J
JJ
Immunology Division, Department of Molecular and Cell Biology, University of California, Berkeley, Berkeley, CA 94720, USA.
Gackstetter
E R
ER
Koshland
M E
ME
D000818:Animals; D018941:Antigens, CD19 / Q000235:genetics; D001402:B-Lymphocytes / Q000166:cytology / Q000276:immunology / Q000378:metabolism*; D001665:Binding Sites; D002460:Cell Line; D004268:DNA-Binding Proteins / Q000235:genetics* / Q000378:metabolism*; D015870:Gene Expression; D005786:Gene Expression Regulation; D005803:Genes, Immunoglobulin; D007143:Immunoglobulin Heavy Chains / Q000235:genetics; D007144:Immunoglobulin J-Chains / Q000235:genetics; D051379:Mice; D009687:Nuclear Proteins / Q000235:genetics* / Q000378:metabolism*; D051757:PAX5 Transcription Factor; D010641:Phenotype; D010950:Plasma Cells / Q000276:immunology / Q000378:metabolism; D011401:Promoter Regions, Genetic; D012045:Regulatory Sequences, Nucleic Acid; D012097:Repressor Proteins / Q000235:genetics / Q000378:metabolism; D014157:Transcription Factors / Q000378:metabolism*; D014162:Transfection
D018941:Antigens, CD19; D004268:DNA-Binding Proteins; D007143:Immunoglobulin Heavy Chains; D007144:Immunoglobulin J-Chains; D009687:Nuclear Proteins; D051757:PAX5 Transcription Factor; C497282:Pax5 protein, mouse; D012097:Repressor Proteins; D014157:Transcription Factors