Effects of nitric oxide synthase inhibition on phospholipid fatty acid composition of brown adipose tissue.
We investigated the effects of nitric oxide synthase inhibition on the phospholipid fatty acid composition of brown adipose tissue by administering chronic N omega-nitro-L-arginine methyl ester (L-NAME) (0.01%) to rats via drinking water. L-NAME caused a significant decrease in C14:0, C16:0, C16:1 and C18:1, with a concomitant increase in C18:0 and C18:2, L-NAME treatment elicited an increase in the unsaturation index. There were no significant changes in other fatty acids including the polyunsaturated fatty acids docosahexaenoate (DHA, C22:6) and arachidonate (C20:4). Polyunsaturated fatty acid linoleate (C18:2) was increased and the arachidonate index decreased in the L-NAME-treated group, suggesting a diminished conversion of C18:2 to C20:4.
Saha
S K
SK
Department of Physiology I, Asahikawa Medical College, Japan. saha@asahikawa-med.ac.jp
Ohno
T
T
Ohinata
H
H
Kuroshima
A
A
D000273:Adipose Tissue / Q000033:anatomy & histology / Q000737:chemistry / Q000187:drug effects; D002001:Adipose Tissue, Brown / Q000033:anatomy & histology / Q000737:chemistry* / Q000187:drug effects; D000818:Animals; D016718:Arachidonic Acid / Q000032:analysis; D004791:Enzyme Inhibitors / Q000494:pharmacology; D005227:Fatty Acids / Q000032:analysis*; D005231:Fatty Acids, Unsaturated / Q000032:analysis; D019787:Linoleic Acid / Q000032:analysis; D008297:Male; D019814:Myristic Acid / Q000032:analysis; D019331:NG-Nitroarginine Methyl Ester / Q000494:pharmacology; D009569:Nitric Oxide / Q000378:metabolism; D019001:Nitric Oxide Synthase / Q000037:antagonists & inhibitors* / Q000378:metabolism; D019301:Oleic Acid / Q000032:analysis; D009929:Organ Size / Q000187:drug effects; D019308:Palmitic Acid / Q000032:analysis; D010743:Phospholipids / Q000032:analysis* / Q000737:chemistry; D051381:Rats; D017208:Rats, Wistar; D013229:Stearic Acids / Q000032:analysis
D004791:Enzyme Inhibitors; D005227:Fatty Acids; D005231:Fatty Acids, Unsaturated; D010743:Phospholipids; D013229:Stearic Acids; D019814:Myristic Acid; D016718:Arachidonic Acid; D019301:Oleic Acid; D019308:Palmitic Acid; D009569:Nitric Oxide; C031183:stearic acid; D019787:Linoleic Acid; D019001:Nitric Oxide Synthase; D019331:NG-Nitroarginine Methyl Ester