A mathematical model of the hsp70 regulation in the cell.
A mathematical model of the regulation process of the heat shock protein hsp70 in the cell is presented. The model describes the damaging effect of elevated temperature on proteins; the interaction of free hsp70 with injured proteins and its chaperone role in nascent protein translation; the relation between the amount of free hsp70 and the formation of the activated trimer form of the heat shock factor protein (HSF); the binding of activated HSF with the heat shock elements on the DNA; the transcription of mRNA of hsp70 and the synthesis of hsp70. The reaction of the model to a temporal rise in temperature shows an initial decline and a subsequent sharp rise to an ultimately increased level of free hsp70 in the cell. The response of the model to both a single and two consecutive heat shocks appears to closely resemble experimental data on hsp70 synthesis. This general agreement demonstrates the structure of the model to be sound and suitable as a basis for further modelling the complex tolerance mechanism of the cell.
Peper
A
A
Laboratory of Medical Physics, University of Amsterdam, The Netherlands.
Grimbergen
C A
CA
Spaan
J A
JA
Souren
J E
JE
van Wijk
R
R
D002470:Cell Survival / Q000502:physiology; D002478:Cells, Cultured; D003198:Computer Simulation; D004247:DNA / Q000378:metabolism; D004268:DNA-Binding Proteins / Q000378:metabolism; D018840:HSP70 Heat-Shock Proteins / Q000378:metabolism / Q000502:physiology*; D006979:Hyperthermia, Induced; D008962:Models, Theoretical; D011485:Protein Binding; D011487:Protein Conformation; D011489:Protein Denaturation; D013696:Temperature; D014158:Transcription, Genetic / Q000235:genetics
D004268:DNA-Binding Proteins; D018840:HSP70 Heat-Shock Proteins; D004247:DNA