title: A novel hyaluronan-based gel in laparoscopic adhesion prevention: preclinical evaluation in an animal model.
abstract: OBJECTIVE
  To evaluate the effectiveness of a crosslinked hyaluronan solution (ACP gel) in the prevention of postsurgical adhesions in laparoscopic surgery.
  
  
  METHODS
  A randomized blinded study using a rabbit model in laparoscopic surgery.
  
  
  METHODS
  A standardized surgical trauma in the rabbit uterine horn to induce adhesion formation.
  
  
  METHODS
  Sixty-four sexually matured female New Zealand white rabbits weighing 2.5 to 3.0 kg and aged 3-4 months.
  
  
  METHODS
  After trauma, group 1 (n = 22) received no treatment, group 2 animals (n = 20) received oxidized-regenerated cellulose (Interceed [TC7]) in group 3 (n = 22) 5 mL of ACP gel were applied on the lesion.
  
  
  METHODS
  Six weeks after laparoscopy, a laparotomy was performed and the adhesions were scored according to Blauer's scoring system.
  
  
  RESULTS
  66% of the untreated animals and 85% of the animals treated with Interceed presented with severe adhesions, whereas only 35% of the ACP gel treatment group had significant adhesions. The mean ( +/- SEM) increased adhesion score was 2.24 +/- 0.26 in the untreated group, 2.45 +/- 0.22 in the Interceed group, and was 1.25 +/- 0.28 in the ACP gel group.
  
  
  CONCLUSIONS
  This study revealed that ACP gel holds promise as a novel resorbable biomaterial for the reduction of postoperative adhesions after laparoscopic surgery.
authors.affiliation: S. Orsola Hospital, Department of Obstetrics and Gynecology, University of Bologna, Italy.
authors.forename: P A
authors.initials: PA
authors.lastname: De Iaco
authors.forename: M
authors.initials: M
authors.lastname: Stefanetti
authors.forename: D
authors.initials: D
authors.lastname: Pressato
authors.forename: S
authors.initials: S
authors.lastname: Piana
authors.forename: M
authors.initials: M
authors.lastname: Doná
authors.forename: A
authors.initials: A
authors.lastname: Pavesio
authors.forename: L
authors.initials: L
authors.lastname: Bovicelli
mesh_terms: D000818:Animals; D001706:Biopsy; D015331:Cohort Studies; D005260:Female; D005782:Gels; D006820:Hyaluronic Acid / Q000737:chemistry*; D010535:Laparoscopy / Q000379:methods*; D011817:Rabbits; D011897:Random Allocation; D000267:Tissue Adhesions / Q000473:pathology / Q000517:prevention & control* / Q000601:surgery; D014599:Uterus / Q000293:injuries / Q000473:pathology / Q000601:surgery
chemical_list: D005782:Gels; D006820:Hyaluronic Acid