title: Assessment using ELISA of the herd immunity levels induced in cattle by foot-and-mouth disease oil vaccines.
abstract: The development of a liquid-phase blocking sandwich ELISA (LPBE) to measure antibodies (Ab) produced in cattle with the O, A and C foot-and-mouth disease virus (FMDV) types of commercial vaccines used in Argentina is described. The test was specific: 99% of naïve cattle sera (n = 130) gave titres below log10 = 1.2, and none had a titre above log10 = 1.5. Comparative studies with serum neutralization test (SNT) using sera from cattle which received one or more vaccine doses is reported. The overall rank correlation coefficient (Spearman's rho, rs) between SNT and LPBE were highly significant (rs > 0.67, P < 0.0001) for all vaccine strains. LBPE Ab titres on sera collected 90 days post vaccination were compared with results of cattle protection tests by applying a logistic regression. The minimum Ab titres at which 85% and 75% of the cattle were protected for each FMDV type were determined in order to interpret field Ab data in terms of protection. Application of this method allows large scale serological examinations to monitor antibody levels in vaccinated animals as an indirect indicator of the FMD control program status in the field. Its use in the evaluation of commercial batches of FMD vaccine is discussed.
authors.affiliation: Instituto de Virología, Centro de Investigación en Ciencias Veterinarias, INTA, Pcia, Buenos Aires, Argentina. eliana@castelar.gov.ar
authors.forename: E N
authors.initials: EN
authors.lastname: Smitsaart
authors.forename: M
authors.initials: M
authors.lastname: Zanelli
authors.forename: I
authors.initials: I
authors.lastname: Rivera
authors.forename: N
authors.initials: N
authors.lastname: Fondevila
authors.forename: D
authors.initials: D
authors.lastname: Compaired
authors.forename: E
authors.initials: E
authors.lastname: Maradei
authors.forename: T
authors.initials: T
authors.lastname: Bianchi
authors.forename: V
authors.initials: V
authors.lastname: O'Donnell
authors.forename: A A
authors.initials: AA
authors.lastname: Schudel
mesh_terms: D000818:Animals; D000914:Antibodies, Viral / Q000097:blood*; D005537:Aphthovirus / Q000276:immunology*; D001118:Argentina / Q000453:epidemiology; D002417:Cattle; D002418:Cattle Diseases / Q000453:epidemiology / Q000276:immunology / Q000517:prevention & control*; D004196:Disease Outbreaks / Q000662:veterinary; D004797:Enzyme-Linked Immunosorbent Assay / Q000662:veterinary; D005536:Foot-and-Mouth Disease / Q000453:epidemiology / Q000276:immunology / Q000517:prevention & control*; D016015:Logistic Models; D009500:Neutralization Tests / Q000662:veterinary; D011817:Rabbits; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D014611:Vaccination / Q000662:veterinary; D014765:Viral Vaccines / Q000276:immunology*
chemical_list: D000914:Antibodies, Viral; D014765:Viral Vaccines