title: Resonance Raman spectroscopy of a light-harvesting protein from the brown alga Laminaria saccharina.
abstract: Resonance Raman spectroscopy of an antenna protein from the brown alga Laminaria saccharina has been used to investigate the molecular structure of this light-harvesting complex (LHC) at the level of its bound pigments, chlorophylls (chl) a and c and the xanthophyll fucoxanthin. Evidence has been obtained for the conservation of pigment structure during the isolation procedure used. Six chl a and two chl c molecules are indicated from the positions and relative contributions of stretching modes of their keto-carbonyl groups. Of special interest is the presence of a population of chls a having a protein-binding conformation highly similar to that seen in antenna proteins from higher plants, possibly indicating a common structural motif within this extended gene family. The eight fucoxanthin molecules evidenced are all in the all-trans conformation; however, one or two have a highly twisted configuration. The results are discussed in terms of common and varying structural features of LHCs in higher plants and algae.
authors.affiliation: Section de Biophysique des Proteines et des Membranes, DBCM/CEA & URA 2096/CNRS, CE-Saclay, 91191 Gif-sur-Yvette, France.
authors.forename: A A
authors.initials: AA
authors.lastname: Pascal
authors.forename: L
authors.initials: L
authors.lastname: Caron
authors.forename: B
authors.initials: B
authors.lastname: Rousseau
authors.forename: K
authors.initials: K
authors.lastname: Lapouge
authors.forename: J
authors.initials: J
authors.lastname: Duval
authors.forename: B
authors.initials: B
authors.lastname: Robert
mesh_terms: D001665:Binding Sites; D002338:Carotenoids / Q000031:analogs & derivatives / Q000737:chemistry; D002734:Chlorophyll / Q000737:chemistry; D000077194:Chlorophyll A; D007795:Laminaria / Q000737:chemistry*; D045342:Light-Harvesting Protein Complexes; D008968:Molecular Conformation; D045322:Photosynthetic Reaction Center Complex Proteins / Q000737:chemistry*; D011487:Protein Conformation; D013059:Spectrum Analysis, Raman; D024341:Xanthophylls
chemical_list: D045342:Light-Harvesting Protein Complexes; D045322:Photosynthetic Reaction Center Complex Proteins; D024341:Xanthophylls; C025164:fucoxanthin; D002734:Chlorophyll; C064041:chlorophyll c; D002338:Carotenoids; D000077194:Chlorophyll A