[Recombinant proteins containing amino acid sequences of two ectatomin chains].
Artificial genes for chains A and B of ectatomin, an Ectatomma tuberculatum ant toxin, were obtained by chemical and enzymic synthesis and cloned into new plasmid vectors. Expression plasmids with the genes of hybrid proteins were constructed containing human interleukin-3 or its terminal 63-mer fragment as well as chains A and B of ectatomin, which are linked via a region containing the cleavage site of specific protease, enterokinase (hybrid proteins IL3ETOXA, IL3ETOXB, ILETOXA, and ILETOXB). Escherichia coli producer strains providing a high yield of IL3ETOXA and IL3ETOXB proteins as inclusion bodies were obtained.
Esipov
R S
RS
Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Moskow, Russia.
Gurevich
A I
AI
Kaiushin
A L
AL
Korosteleva
M D
MD
Miroshnikov
A I
AI
Shevchenko
L V
LV
Pluzhnikov
K A
KA
Grishin
E V
EV
D000595:Amino Acid Sequence; D000862:Ant Venoms / Q000096:biosynthesis* / Q000737:chemistry / Q000235:genetics; D001483:Base Sequence; D004586:Electrophoresis; D004591:Electrophoresis, Polyacrylamide Gel; D004765:Enteropeptidase / Q000737:chemistry; D004926:Escherichia coli / Q000235:genetics; D005822:Genetic Vectors; D006801:Humans; D007377:Interleukin-3 / Q000235:genetics; D008969:Molecular Sequence Data; D010957:Plasmids; D016133:Polymerase Chain Reaction; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000737:chemistry / Q000235:genetics
D000862:Ant Venoms; D007377:Interleukin-3; D011994:Recombinant Proteins; C088426:ectatomin protein, Ectatomma tuberculatum; D004765:Enteropeptidase
Rekombinantnye belki, soderzhashchie aminokislotnye posledovatel'nosti dvukh tsepeĭ éktatomina.