title: Analysis of DNA ploidy and expression of tumour-associated antigens on human oral carcinomas xenografted in nude mice.
abstract: Human squamous cell carcinomas (SCC) of the oral cavity were successfully established as xenografts in nude mice. Tumours with higher malignancy scores and involvement of lymph nodes in patients were more readily accepted as xenografts in nude mice. The xenografted tumours were characterised with respect to morphology, histology, DNA index and expression of tumour-associated antigens (TAA). Flow cytometric analysis of cellular DNA content revealed that many of the xenografts retained the parent tumour DNA pattern while some of the xenografts showed progression to aneuploidy. All the xenografted tumours expressed TAA recognised by monoclonal antibody (MAb) 3F8E3. On Western blotting, MAb 3F8E3 recognised proteins of molecular weight 62-64 kDa on parent and xenografted tumours. In general, the xenografts reflect many of the characteristics of the tumours from which they were derived and may provide a useful model for investigating newer approaches of treatment and diagnosis.
authors.affiliation: Cellular Immunology Unit, Cancer Research Institute, Parel, Mumbai, India.
authors.forename: A
authors.initials: A
authors.lastname: Damodaran
authors.forename: S G
authors.initials: SG
authors.lastname: Gangal
authors.forename: D
authors.initials: D
authors.lastname: Pichan
authors.forename: K
authors.initials: K
authors.lastname: Amin
authors.forename: A R
authors.initials: AR
authors.lastname: Fakih
authors.forename: N
authors.initials: N
authors.lastname: Jambhekar
authors.forename: R
authors.initials: R
authors.lastname: Rao
authors.forename: S V
authors.initials: SV
authors.lastname: Chiplunkar
mesh_terms: D000818:Animals; D000951:Antigens, Neoplasm / Q000032:analysis*; D002294:Carcinoma, Squamous Cell / Q000235:genetics* / Q000276:immunology / Q000473:pathology; D004273:DNA, Neoplasm / Q000032:analysis; D004195:Disease Models, Animal; D005260:Female; D006801:Humans; D051379:Mice; D008819:Mice, Nude; D009062:Mouth Neoplasms / Q000235:genetics* / Q000276:immunology / Q000473:pathology; D009368:Neoplasm Transplantation; D011003:Ploidies; D016196:S Phase; D014183:Transplantation, Heterologous
chemical_list: D000951:Antigens, Neoplasm; D004273:DNA, Neoplasm