Domain requirements for the Dock adapter protein in growth- cone signaling.
Tyrosine phosphorylation has been implicated in growth-cone guidance through genetic, biochemical, and pharmacological studies. Adapter proteins containing src homology 2 (SH2) domains and src homology 3 (SH3) domains provide a means of linking guidance signaling through phosphotyrosine to downstream effectors regulating growth-cone motility. The Drosophila adapter, Dreadlocks (Dock), the homolog of mammalian Nck containing three N-terminal SH3 domains and a single SH2 domain, is highly specialized for growth-cone guidance. In this paper, we demonstrate that Dock can couple signals in either an SH2-dependent or an SH2-independent fashion in photoreceptor (R cell) growth cones, and that Dock displays different domain requirements in different neurons.
Rao
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Department of Biological Chemistry, Molecular Biology Institute, The School of Medicine, University of California, Los Angeles, CA 90095-1662, USA.
Zipursky
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D048868:Adaptor Proteins, Signal Transducing; D000818:Animals; D030801:Animals, Genetically Modified; D001369:Axons / Q000502:physiology* / Q000648:ultrastructure; D019976:Cloning, Organism; D003433:Crosses, Genetic; D004330:Drosophila / Q000235:genetics / Q000502:physiology; D029721:Drosophila Proteins; D005123:Eye / Q000294:innervation; D017952:Ganglia, Invertebrate / Q000502:physiology*; D006801:Humans; D007814:Larva; D008322:Mammals; D016254:Mutagenesis, Insertional; D009412:Nerve Fibers / Q000502:physiology* / Q000648:ultrastructure; D009419:Nerve Tissue Proteins / Q000096:biosynthesis / Q000737:chemistry / Q000378:metabolism*; D015513:Oncogene Proteins / Q000737:chemistry / Q000378:metabolism; D011994:Recombinant Proteins / Q000737:chemistry / Q000378:metabolism; D015398:Signal Transduction
D048868:Adaptor Proteins, Signal Transducing; D029721:Drosophila Proteins; C078332:Nck protein; D009419:Nerve Tissue Proteins; D015513:Oncogene Proteins; D011994:Recombinant Proteins; C099753:dock protein, Drosophila