title: High pressure induces G2 arrest in murine erythroleukemia cells.
abstract: The effect of high pressure on proliferation and cell cycle progression was examined using murine erythroleukemia (MEL) cells. The MEL cells were exposed to high pressures (0.1-130 MPa) and then cultured for 5 days at atmospheric pressure. The proliferation of MEL cells was unaffected up to 60 MPa, but was suppressed at 80-110 MPa. Above 120 MPa, the cells were fragmented. The cell cycle analysis of 80 MPa-treated MEL cells showed that the cells in S phase are most sensitive to high pressure and they arrest in G2 phase. Interestingly, G2-arrested cells reinitiated DNA synthesis, resulting in giant cells with high DNA contents. Furthermore, when such G2-arrested cells were exposed to caffeine, premature mitosis, characterized by chromosome pulverization, was observed. These results suggest that the suppression of proliferation in high-pressure-treated MEL cells is associated with G2 arrest following S phase delay. Thus, it seems valuable to apply high pressure to the investigation of the cell cycle.
authors.affiliation: Department of Chemistry, Faculty of Science, Fukuoka University.
authors.forename: M
authors.initials: M
authors.lastname: Matsumoto
authors.forename: T
authors.initials: T
authors.lastname: Yamaguchi
authors.forename: Y
authors.initials: Y
authors.lastname: Fukumaki
authors.forename: R
authors.initials: R
authors.lastname: Yasunaga
authors.forename: S
authors.initials: S
authors.lastname: Terada
mesh_terms: D000225:Adenine / Q000031:analogs & derivatives / Q000494:pharmacology; D000818:Animals; D002110:Caffeine / Q000494:pharmacology; D002453:Cell Cycle / Q000187:drug effects; D002455:Cell Division; D016195:G2 Phase; D004915:Leukemia, Erythroblastic, Acute; D051379:Mice; D008940:Mitotic Index; D011312:Pressure; D014407:Tumor Cells, Cultured
chemical_list: D002110:Caffeine; C001020:N(6),N(6)-dimethyladenine; D000225:Adenine