M-cell surface beta1 integrin expression and invasin-mediated targeting of Yersinia pseudotuberculosis to mouse Peyer's patch M cells.
Quantitative analysis of Yersinia pseudotuberculosis infection of murine gut loops revealed that significantly more wild-type bacteria associated with Peyer's patch M cells than with dome enterocytes or goblet cells. An invasin-deficient mutant was significantly attenuated for M-cell invasion, while beta1 integrin expression was demonstrated in the apical membranes of M cells but not enterocytes. M-cell targeting by Yersinia pseudotuberculosis in vivo may, therefore, be mediated primarily by the interaction of invasin with cell surface beta1 integrins.
Clark
M A
MA
Department of Physiological Sciences, Medical School, University of Newcastle upon Tyne, United Kingdom. Ann.Clark@newcastle.ac.uk
Hirst
B H
BH
Jepson
M A
MA
D018829:Adhesins, Bacterial; D000818:Animals; D001426:Bacterial Proteins / Q000502:physiology*; D005260:Female; D019012:Integrin beta1 / Q000502:physiology*; D051379:Mice; D008807:Mice, Inbred BALB C; D010581:Peyer's Patches / Q000166:cytology / Q000382:microbiology* / Q000648:ultrastructure; D015011:Yersinia pseudotuberculosis / Q000472:pathogenicity*
D018829:Adhesins, Bacterial; D001426:Bacterial Proteins; D019012:Integrin beta1; C053476:invasin, Yersinia