Simultaneous detection of antigens and specific DNA sequences of human papillomavirus in uterine cervical biopsy specimens. Description of a double-labelling technique.
We studied 70 uterine cervical biopsy specimens with a histological diagnosis compatible with human papillomavirus (HPV) infection. We carried out immunohistochemical and in situ hybridization techniques and selected 22 specimens that had given a positive result with both techniques. We then used a double-labelling technique (a combination of immunohisto-chemistry and in situ hybridization) to detect simultaneously viral antigens and specific gene sequences of HPV. With this technique we found three different cell types in the tissue: (1) cells with black nuclei, positive by immunohisto-chemistry and in situ hybridization; (2) cells with red nuclei, negative by immunohistochemistry and positive by in situ hybridization; and (3) cells with light blue nuclei, negative by both techniques. In this study we describe this technique; and we believe that the simultaneous detection of viral antigens and specific gene sequences of HPV may be very useful in the study of the virus-cell interaction.
Gómez
F
F
Servicio de Anatomía Patológica. Centro Nacional de Investigación Clínica y Medicina Preventiva, Instituto de Salud Carlos III, Madrid.
Roldán
M
M
Corcuera
M T
MT
Picazo
A
A
Muñoz
E
E
Alonso
M J
MJ
D000957:Antigens, Viral, Tumor / Q000032:analysis*; D001483:Base Sequence; D002294:Carcinoma, Squamous Cell / Q000473:pathology / Q000821:virology; D015344:DNA Probes, HPV; D004279:DNA, Viral / Q000032:analysis*; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D017403:In Situ Hybridization; D027383:Papillomaviridae / Q000235:genetics* / Q000276:immunology* / Q000302:isolation & purification; D030361:Papillomavirus Infections / Q000473:pathology* / Q000821:virology; D014412:Tumor Virus Infections / Q000473:pathology* / Q000821:virology; D002583:Uterine Cervical Neoplasms / Q000473:pathology / Q000821:virology; D014594:Uterine Neoplasms / Q000473:pathology / Q000821:virology
D000957:Antigens, Viral, Tumor; D015344:DNA Probes, HPV; D004279:DNA, Viral