title: Removal of methylene blue from plasma via an adsorbent filter.
abstract: OBJECTIVE
  To evaluate adsorbent filtration of methylene blue (MB) and leukocytes from plasma.
  
  
  METHODS
  Plasma (750 ml) from apheresis of 10 normal subjects was split into three aliquots: control (A), filtration (B) and MB addition (to 1 microM), phototreatment and filtration (C). Biochemical and coagulation tests were performed: units A and B were reinfused.
  
  
  RESULTS
  Filtration reduced MB to undetectable (< 0.05 microM) levels and leukocytes by 3 log10. Biochemical analytes were unchanged. The partial thromboplastin time was prolonged with MB addition (11 +/- 13%) or filtration (26 +/- 12%, p < 0.05), but the effects were not additive. Autologous transfusion was well tolerated.
  
  
  CONCLUSIONS
  Adsorbent filtration can reduce residual MB to undetectable levels and yields a component suitable for transfusion.
authors.affiliation: Department of Pathology, Dartmouth-Hitchcock Medical Center, Lebanon, NH 03756, USA. james.p.aubuchon@hitchcock.org
authors.forename: J P
authors.initials: JP
authors.lastname: AuBuchon
authors.forename: C
authors.initials: C
authors.lastname: Pickard
authors.forename: L
authors.initials: L
authors.lastname: Herschel
authors.forename: J L
authors.initials: JL
authors.lastname: O'Connor
authors.forename: E
authors.initials: E
authors.lastname: Lee
mesh_terms: D000327:Adsorption; D001804:Blood Transfusion, Autologous; D005260:Female; D005340:Fibrinogen / Q000032:analysis; D005374:Filtration / Q000295:instrumentation* / Q000379:methods; D006403:Hematologic Tests; D006801:Humans; D007958:Leukocyte Count; D008297:Male; D008751:Methylene Blue / Q000032:analysis*; D010314:Partial Thromboplastin Time; D010949:Plasma / Q000737:chemistry* / Q000821:virology; D011517:Prothrombin Time; D012016:Reference Values
chemical_list: D005340:Fibrinogen; D008751:Methylene Blue