Characterization of a new EBV-associated nasopharyngeal carcinoma cell line.
Only a few reports exist of cytogenetic studies in nasopharyngeal carcinoma (NPC), probably because of the technical limitations of culturing solid tumors. We established a cell line (cell-666) that was derived from an NPC xenograft growing in athymic nude mice. Epstein-Barr virus (EBV) infection is one of the important etiologic factors of NPC. This cell line has been shown to have the consistent presence of EBV by Epstein-Barr virus encoded RNAs (EBER) in situ hybridization. Furthermore, aberrant methylation of 5'CpG island of p16 gene was detected. Thus, this cell line represents a valuable model for the study of NPC tumorigenecity. The karyotype of this newly established cell line is: 45,X,del(X)(q24),-5der(5)(5pter-->5q31::5q13-->+ ++5q31::5p12-->5pter),der(6;?) (q10;p10),add(7)(p22), + 9, del(11)(q13),add(12)(p13),-14,-18,-21, + 2mar. Fluorescence in situ hybridization analyses were performed using whole chromosome painting probes of chromosomes 5, 6, 11, and 12.
Hui
A B
AB
Department of Anatomical and Cellular Pathology, Prince of Wales Hospital, Chinese University of Hong Kong, Shatin, N.T., People's Republic of China.
Cheung
S T
ST
Fong
Y
Y
Lo
K W
KW
Huang
D P
DP
D000818:Animals; D002869:Chromosome Aberrations; D006566:Herpesviridae Infections / Q000235:genetics*; D004854:Herpesvirus 4, Human / Q000302:isolation & purification*; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D007621:Karyotyping; D051379:Mice; D008819:Mice, Nude; D009303:Nasopharyngeal Neoplasms / Q000235:genetics / Q000821:virology*; D009368:Neoplasm Transplantation; D012367:RNA, Viral / Q000032:analysis; D014407:Tumor Cells, Cultured; D014412:Tumor Virus Infections / Q000235:genetics*
D012367:RNA, Viral