Mouse spermatogenic cell-specific type 1 hexokinase (mHk1-s) transcripts are expressed by alternative splicing from the mHk1 gene and the HK1-S protein is localized mainly in the sperm tail.
Unique type 1 hexokinase (HK1) mRNAs are present in mouse spermatogenic cells (mHk1-s). They encode a spermatogenic cell-specific sequence region (SSR) but not the porin-binding domain (PBD) necessary for HK1 binding to porin on the outer mitochondrial membrane. This study determined the origin of the multiple Hk1-s transcripts in mouse spermatogenic cells and verified that they are translated in mouse spermatogenic cells. It also showed that a single mHk1 gene encodes the mHk1 transcripts of somatic cells and the mHk1-sa and mHk1-sb transcripts of spermatogenic cells, that alternative exons are used during mHk1 gene expression in mouse spermatogenic cells, and that mHK1-S is translated in mouse spermatogenic cells and is localized mainly with the fibrous sheath in the tail region, not with the mitochondria in the midpiece of mouse sperm.
Mori
C
C
Department of Anatomy and Developmental Biology, Faculty of Medicine, Kyoto University, Japan. mori@med.kyoto-u.ac.jp
Nakamura
N
N
Welch
J E
JE
Gotoh
H
H
Goulding
E H
EH
Fujioka
M
M
Eddy
E M
EM
D017398:Alternative Splicing / Q000235:genetics*; D000818:Animals; D000906:Antibodies / Q000737:chemistry; D001483:Base Sequence; D005091:Exons; D018507:Gene Expression Regulation, Developmental; D006593:Hexokinase / Q000235:genetics* / Q000276:immunology; D008297:Male; D051379:Mice; D008813:Mice, Inbred ICR; D008969:Molecular Sequence Data; D013082:Sperm Tail / Q000201:enzymology*; D013091:Spermatogenesis / Q000235:genetics*; D013737:Testis / Q000201:enzymology; D014158:Transcription, Genetic
D000906:Antibodies; D006593:Hexokinase