Alternative splicing and tissue specific expression of the 5' truncated bCCE 1 variant bCCE 1delta514.
In many non-excitable as well as electrically excitable cells, depletion of intracellular Ca2+ stores after stimulation of G protein coupled receptors or receptor tyrosine kinases is followed by Ca2+ entry across the plasma membrane, a mechanism referred to as capacitative calcium entry (CCE) [Putney, J.W., Cell Calcium 11 (1990) 611-624; Fasolato, C. et al., Trends Pharmacol Sci. 15 (1994) 77-83]. Recently, we reported that bCCE 1, a homologue of the Drosophila protein trp, exhibits the characteristics of CCE channels [Philipp, S. et al., EMBO J. 15 (1996) 6166-6171]. In this study, we report the cloning of a 5' truncated splice variant (bCCE 1delta514) of the full-length bCCE 1. The bCCE 1delta514 cDNA encodes a protein of 486 amino acids with the ATG triplet encoding M514 of bCCE 1 as translation initiation codon and, therefore, comprises two putative transmembrane segments corresponding to the predicted transmembrane segments 5 and 6 of bCCE 1. bCCE 1delta514 transcripts appear to be specifically expressed in the adrenal gland and genome analysis reveals an alternative splice site within an exon of the CCE 1 gene leading to the formation of bCCE 1delta514.
Freichel
M
M
Institut für Pharmakologie und Toxikologie, Universität des Saarlandes, Homburg, Germany.
Wissenbach
U
U
Philipp
S
S
Flockerzi
V
V
D000311:Adrenal Glands / Q000378:metabolism; D017398:Alternative Splicing; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D015220:Calcium Channels / Q000096:biosynthesis / Q000235:genetics*; D027682:Cation Transport Proteins; D002417:Cattle; D003001:Cloning, Molecular; D051379:Mice; D008969:Molecular Sequence Data; D017386:Sequence Homology, Amino Acid; D050052:TRPC Cation Channels
D015220:Calcium Channels; D027682:Cation Transport Proteins; D050052:TRPC Cation Channels; C489571:TRPC4 ion channel