title: Effect of low-power laser irradiation on the mechanical properties of bone fracture healing in rats.
abstract: OBJECTIVE
  Low-power laser irradiation (LPLI) has been found to have a positive effect on bone fracture healing in animal models, based on morphogenic, biochemical, roentgenographic, and electron microscopic measurements. We investigated the effect of LPLI on bone fracture healing in rats using biomechanical methods.
  
  
  METHODS
  Two groups of male Wistar rats, divided in a randomized block design in a blinded fashion, each consisting of 25 animals, were subjected to anesthesia and tibial bone fracture with internal fixation. The first group was treated with LPLI (HeNe laser 632.8 nm, 35 mW), applied transcutaneously over 30 minutes to the area of the fracture daily for 14 days. The second group served as a control. After 4 weeks, the tibia was removed and tested at tension up to failure (by a Lloyd LR 50K testing apparatus, U.K.) in 16 rats from group I and 15 from group II. The maximal load at failure, the structural stiffness of the tibia (callus stiffness), and the extension maximal load were measured.
  
  
  RESULTS
  The maximal load at failure and the structural stiffness of the tibia were found to be elevated significantly in the irradiated group (P = .014 and P = .0023, respectively), whereas the extension maximal load was reduced (P = .015). In addition, gross non-union was found in four fractures in the control group, compared to none in the irradiated group.
  
  
  CONCLUSIONS
  These results suggest that LPLI treatment may play a role in enhancing bone healing.
authors.affiliation: Department of Orthopedic Surgery B, Tel Aviv Sourasky Medical Center, Israel.
authors.forename: E J
authors.initials: EJ
authors.lastname: Luger
authors.forename: S
authors.initials: S
authors.lastname: Rochkind
authors.forename: Y
authors.initials: Y
authors.lastname: Wollman
authors.forename: G
authors.initials: G
authors.lastname: Kogan
authors.forename: S
authors.initials: S
authors.lastname: Dekel
mesh_terms: D000818:Animals; D005593:Fracture Fixation, Internal; D017102:Fracture Healing / Q000528:radiation effects*; D053685:Laser Therapy; D008297:Male; D051381:Rats; D017208:Rats, Wistar; D013314:Stress, Mechanical; D013978:Tibial Fractures / Q000503:physiopathology / Q000532:radiotherapy* / Q000601:surgery