title: Antibody response to Chlamydia pneumoniae infection in children with respiratory illness.
abstract: Serologic diagnosis of Chlamydia pneumoniae infection has been based on the microimmunofluorescence test (MIF). However, recent prospective studies in children have found that >50% infected with C. pneumoniae failed to develop any antibodies detectable by MIF. In this study, single sera from 46 culture-positive and 42 culture-negative children with respiratory infection and known MIF status were examined by immunoblotting. Forty-one (89.1%) of the single sera from culture-positive and 27 (64.3%) from culture-negative children reacted to C. pneumoniae antigens in immunoblot. C. pneumoniae proteins most frequently recognized by sera from culture-positive patients were at 101-102, 72-76, 50-52, 48-49, 43-44, 41-42, and 30-31 kDa. However, there did not appear to be a correlation of specific band patterns and culture status.
authors.affiliation: Department of Pediatrics, State University of New York at Brooklyn, 11203-2098, USA.
authors.forename: A
authors.initials: A
authors.lastname: Kutlin
authors.forename: P M
authors.initials: PM
authors.lastname: Roblin
authors.forename: M R
authors.initials: MR
authors.lastname: Hammerschlag
mesh_terms: D000293:Adolescent; D000907:Antibodies, Bacterial / Q000097:blood*; D001426:Bacterial Proteins / Q000276:immunology; D002648:Child; D002675:Child, Preschool; D002690:Chlamydia Infections / Q000175:diagnosis* / Q000276:immunology; D016993:Chlamydophila pneumoniae / Q000276:immunology* / Q000302:isolation & purification; D002986:Clinical Trials as Topic; D005455:Fluorescent Antibody Technique; D006801:Humans; D015151:Immunoblotting; D012141:Respiratory Tract Infections / Q000175:diagnosis* / Q000276:immunology; D012189:Retrospective Studies
chemical_list: D000907:Antibodies, Bacterial; D001426:Bacterial Proteins