Cytochrome c potentiates fatty acid-induced cyclosporin A-sensitive permeability transition in liver mitochondria.
Addition of 0.5-5 microM cytochrome c and 30 microM lauric acid to rat liver mitochondria stimulated the respiration rate. Stimulation of respiration by cytochrome c was prevented by cyclosporin A and was not observed in the absence of lauric acid. High amplitude swelling of mitochondria in the presence of lauric acid was also potentiated by cytochrome c; this effect was prevented by cyclosporin A or by EGTA. Polylysine (0.35 microM) induced cyclosporin A-insensitive high amplitude swelling of mitochondria. Polylysine-induced swelling was stimulated by lauric acid and cytochrome c; the effect was prevented by cyclosporin A. Incubation of mitochondria with 30 microM lauric acid resulted in an increase in cytochrome c concentration in the incubation medium. Added cytochrome c is supposed to potentiate the laurate-induced cyclosporin A-sensitive permeability transition in cytochrome c-depleted mitochondria.
Amerkhanov
Z G
ZG
Institute for Cell Biophysics, Russian Academy of Sciences, Pushchino, Moscow Region, Russia. amerkhanov@venus.iteb.serpukhov.su
Mokhova
E N
EN
D000818:Animals; D016572:Cyclosporine / Q000494:pharmacology*; D003574:Cytochrome c Group / Q000378:metabolism*; D005227:Fatty Acids / Q000378:metabolism*; D007425:Intracellular Membranes / Q000187:drug effects / Q000378:metabolism; D008930:Mitochondria, Liver / Q000187:drug effects* / Q000378:metabolism; D008933:Mitochondrial Swelling / Q000187:drug effects; D010100:Oxygen / Q000378:metabolism; D010539:Permeability / Q000187:drug effects; D051381:Rats
D003574:Cytochrome c Group; D005227:Fatty Acids; D016572:Cyclosporine; D010100:Oxygen