Ladder beam and camera video recording system for evaluating forelimb and hindlimb deficits after sensorimotor cortex injury in rats.
Hindlimb and forelimb deficits in rats caused by sensorimotor cortex lesions are frequently tested by using the narrow flat beam (hindlimb), the narrow pegged beam (hindlimb and forelimb) or the grid-walking (forelimb) tests. Although these are excellent tests, the narrow flat beam generates non-parametric data so that using more powerful parametric statistical analyses are prohibited. All these tests can be difficult to score if the rat is moving rapidly. Foot misplacements, especially on the grid-walking test, are indicative of an ongoing deficit, but have not been reliably and accurately described and quantified previously. In this paper we present an easy to construct and use horizontal ladder-beam with a camera system on rails which can be used to evaluate both hindlimb and forelimb deficits in a single test. By slow motion videotape playback we were able to quantify and demonstrate foot misplacements which go beyond the recovery period usually seen using more conventional measures (i.e. footslips and footfaults). This convenient system provides a rapid and reliable method for recording and evaluating rat performance on any type of beam and may be useful for measuring sensorimotor recovery following brain injury.
Soblosky
J S
JS
Department of Neurosurgery, Louisiana State University Medical Center, New Orleans 70112, USA. jsoblo@lsumc.edu
Colgin
L L
LL
Chorney-Lane
D
D
Davidson
J F
JF
Carey
M E
ME
D000818:Animals; D001930:Brain Injuries / Q000503:physiopathology*; D001931:Brain Mapping; D004867:Equipment Design; D005552:Forelimb / Q000294:innervation*; D006614:Hindlimb / Q000294:innervation*; D008124:Locomotion / Q000502:physiology*; D008297:Male; D009043:Motor Activity; D009044:Motor Cortex / Q000293:injuries / Q000502:physiology / Q000503:physiopathology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D014741:Video Recording / Q000295:instrumentation / Q000379:methods