title: Indirect demonstration of the lifetime function of human thymus.
abstract: The aim of this study was to test the hypothesis that human thymus maintains its function as the site of early T cell development throughout life, but to a progressively diminishing extent. Mononuclear cell suspensions prepared from the samples of 39 human thymuses were analysed for the total number of cells per gram of thymus tissue, percentage of single marker-positive CD2, CD4 and CD8 cells, percentages of double-positive CD4CD8 and CD2CD8 cells, double-negative CD4CD8 cells, absolute numbers of these cells per gram of tissue, and extent of the in vitro proliferation upon stimulation with concanavalin A (Con A), phytohaemagglutinin (PHA) and pokeweed mitogen (PWM) mitogens. The main outcome measures were flow cytometric data on thymus lymphoid cell composition (according to CD classification), expressed as percentages and numbers of cells per gram of thymus tissue. The total number of mononuclear cells expressed per gram of thymus tissue exponentially decreased with age. The slope of none of the analysed cell subpopulations differed from the slope of the line constructed for age-related decline of the total number of mononuclear cells (-0.024 on a semilogarithmic scale). The thymuses of all ages contained all analysed cell subpopulations in approximately the same proportions: percentages of these cell subpopulations did not change with age, except for all CD4+ (P=0.017) and double-positive CD4+CD8+ (P=0.016) cells, which tended to decrease with age. The extent of proliferation of thymus cells upon stimulation with T and B cell mitogens was unrelated to age. We conclude that the thymus retains its function as the site of differentiation of T lymphocytes throughout life. With respect to the number of involved lymphoid cells, the function exponentially decreases with age.
authors.affiliation: Department of Immunology, Institute of Clinical Laboratory Diagnosis, Zagreb University Hospital Center and School of Medicine, Croatia.
authors.forename: M
authors.initials: M
authors.lastname: Marusić
authors.forename: M
authors.initials: M
authors.lastname: Turkalj-Kljajić
authors.forename: M
authors.initials: M
authors.lastname: Petrovecki
authors.forename: B
authors.initials: B
authors.lastname: Uzarević
authors.forename: M
authors.initials: M
authors.lastname: Rudolf
authors.forename: D
authors.initials: D
authors.lastname: Batinić
authors.forename: R
authors.initials: R
authors.lastname: Ugljen
authors.forename: D
authors.initials: D
authors.lastname: Anić
authors.forename: Z
authors.initials: Z
authors.lastname: Cavar
authors.forename: I
authors.initials: I
authors.lastname: Jelić
authors.forename: B
authors.initials: B
authors.lastname: Malenica
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000375:Aging / Q000502:physiology*; D002648:Child; D002675:Child, Preschool; D005260:Female; D006801:Humans; D007223:Infant; D008213:Lymphocyte Activation / Q000502:physiology; D008297:Male; D008875:Middle Aged; D013601:T-Lymphocytes / Q000166:cytology / Q000502:physiology; D013950:Thymus Gland / Q000166:cytology / Q000502:physiology*