Advances in quantitative PCR technology: 5' nuclease assays.
The development of 5' nuclease assays represents a significant advance in nucleic acid quantitation. This approach utilizes the 5'-3' exonuclease activity of Thermus aquaticus (Taq) polymerase to cleave a dual-labelled probe annealed to a target sequence during amplification. The release of a fluorogenic tag from the 5' end of the probe is proportional to the target sequence concentration (copy number), and can be measured either at endpoint (post-amplification), or in real time', where the increase in emission intensity is followed on a per-cycle basis.
Lie
Y S
YS
ViroLogic, Inc, South San Francisco, California, USA.
Petropoulos
C J
CJ
D000483:Alleles; D005090:Exodeoxyribonucleases; D018628:Gene Dosage; D007700:Kinetics; D016133:Polymerase Chain Reaction / Q000295:instrumentation / Q000379:methods*; D012313:RNA
D012313:RNA; D005090:Exodeoxyribonucleases