Detoxification of an alkylating drug, N,N-bis(2-chloroethyl)-p-aminophenylbutyric acid (chlorambucil), in human gastric juice and saliva.
N,N-Bis(2-chloroethyl)-p-aminophenylbutyric acid (chlorambucil, 1) is an orally administered drug widely used in the chemotherapy of chronic lymphocytic leukemia. It is converted in gastric juice into three stable metabolites, which were characterized as N,N-bis(2-hydroxyethyl)-p-aminophenylbutyric acid (4), N-(2-hydroxyethyl)-N-[2-(thiocyano)ethyl]-p-aminophenylbutyric+ ++ acid (5), and N,N-bis[2-(thiocyano)ethyl]-p-aminophenylbutyric acid (6). 4 is the product of chloroambucil hydrolysis, while 5 and 6 are results of the reaction of 1 with saliva-derived thiocyanate ion. The destabilizing effect of low gastric oxonium ion concentration on 1 is also demonstrated.
Hovinen
J
J
Department of Chemistry, University of Turku, Wallac Oy, Finland. jari.hovinen@wallac.fi
Silvennoinen
R
R
Vilpo
J
J
D000284:Administration, Oral; D018906:Antineoplastic Agents, Alkylating / Q000378:metabolism* / Q000493:pharmacokinetics; D001682:Biological Availability; D002699:Chlorambucil / Q000378:metabolism* / Q000493:pharmacokinetics; D005750:Gastric Juice / Q000737:chemistry; D006801:Humans; D007700:Kinetics; D012463:Saliva / Q000737:chemistry
D018906:Antineoplastic Agents, Alkylating; D002699:Chlorambucil