Effects of photodynamic therapy combined with methotrexate on C6 rat glioma cells: a preliminary study.
This study was undertaken to determine if methotrexate (MTX) is effective against tumor cells surviving photodynamic therapy (PDT). C6 rat glioma cells were exposed to Photofrin and irradiated at 630 nm using power densities of 1.2 or 4.8 J/cm2 to simulate the conditions for cells in solid tumors which survive PDT. Cells were then treated with MTX at 5.0, 0.5, or 0.05 microM concentrations. MTT assay of cell proliferation was performed at 24, 48, 72, and 96 h postirradiation. During the first 48 h of incubation, MTX alone was more effective than PDT and MTX. After 48 h, the combination treatment was more effective. Further studies of combined PDT and chemotherapy are warranted.
LoRusso
A P
AP
Department of Surgery, Rochester General Hospital, University of Rochester School of Medicine and Dentistry, New York 14621, USA.
Yu
W
W
Naim
J O
JO
Lanzafame
R J
RJ
D000818:Animals; D000970:Antineoplastic Agents / Q000008:administration & dosage / Q000627:therapeutic use*; D017323:Dihematoporphyrin Ether / Q000627:therapeutic use*; D019008:Drug Resistance, Neoplasm; D004359:Drug Therapy, Combination; D005069:Evaluation Studies as Topic; D005910:Glioma / Q000188:drug therapy* / Q000628:therapy; D008727:Methotrexate / Q000008:administration & dosage / Q000627:therapeutic use*; D010778:Photochemotherapy; D017319:Photosensitizing Agents / Q000008:administration & dosage / Q000627:therapeutic use*; D051381:Rats; D011919:Rats, Inbred Strains; D013997:Time Factors; D014407:Tumor Cells, Cultured / Q000187:drug effects / Q000528:radiation effects
D000970:Antineoplastic Agents; D017319:Photosensitizing Agents; D017323:Dihematoporphyrin Ether; D008727:Methotrexate