Allergen-induced migration of human cells in allergic severe combined immunodeficiency mice.
Recently, we have shown that severe combined immunodeficiency (SCID) mice, intraperitoneally reconstituted with peripheral blood mononuclear cells (PBMC) from Dermatophagoides pteronyssinus (Dpt)-sensitive patients, produced human IgE and developed a pulmonary inflammatory-type reaction after exposure to allergen aerosol. In order to understand the potential mechanisms involved in the human cell migration in SCID mice, we analysed their phenotypic profile in the lungs, spleen and thymus, 2 months after Dpt inhalation. The human cell recruitment in these organs was found to be allergen-dependent as CD45+ human cells were only detected in hu-SCID mice after Dpt exposure. The composition of the pulmonary human T-cell infiltrate, preferentially memory (CD45RO), activated (human leucocyte antigen (HLA)-DR) and CD4+ cells, was similar to that described in asthmatic patients. However, CD20+ B cells were predominately recruited in the spleen and thymus and may be IgE-producing cells in the spleen. In the lungs, the percentage of human leucocytes expressing the alpha-chain of the lymphocyte function-associated antigen-1 (LFA-1) (CD11a) was higher than those of CD49d+ or CD54+ cells, in contrast to the spleen and thymus, suggesting a potential role of LFA-1 in the human cell migration towards SCID mice lung. In conclusion, this model could be useful in the study of factors implicated in the cellular migration towards the lymphoid organs during an allergic reaction.
Duez
C
C
INSERM U 416, Lille, France.
Akoum
H
H
Marquillies
P
P
Cesbron
J Y
JY
Tonnel
A B
AB
Pestel
J
J
D000280:Administration, Inhalation; D000485:Allergens / Q000276:immunology* / Q000494:pharmacology; D000818:Animals; D015703:Antigens, CD / Q000032:analysis; D039741:Antigens, Dermatophagoides; D017690:Cell Transplantation; D002634:Chemotaxis, Leukocyte; D006023:Glycoproteins / Q000276:immunology* / Q000494:pharmacology; D006801:Humans; D016130:Immunophenotyping; D017493:Leukocyte Common Antigens / Q000276:immunology; D008168:Lung / Q000166:cytology / Q000276:immunology; D051379:Mice; D016513:Mice, SCID; D008925:Mites / Q000276:immunology*; D013154:Spleen / Q000166:cytology / Q000276:immunology; D013601:T-Lymphocytes / Q000276:immunology; D013950:Thymus Gland / Q000166:cytology / Q000276:immunology
D000485:Allergens; D015703:Antigens, CD; D039741:Antigens, Dermatophagoides; D006023:Glycoproteins; D017493:Leukocyte Common Antigens