title: Effects of macrophage transplantation in the injured adult rat spinal cord: a combined immunocytochemical and biochemical study.
abstract: Early and robust invasion by macrophages may be one of the reasons why axonal regeneration is more effective in the PNS than in the CNS. Therefore, we have grafted autologous peritoneal macrophages labeled with fluorescent latex microspheres into spinal cord compression lesions. At various survival times, we have studied their effect on the expression of neuronal (neurofilaments [NF], calcitonin gene-related peptide [CGRP], 5-hydroxytryptamine [5-HT]) and nonneuronal markers (myelin-associated glycoprotein [MAG], glial fibrillary acidic protein [GFAP], laminin) by using semiquantitative Western blot and immunohistochemical techniques. After 1 month, we observed a significant decrease of the expression of MAG as well as an important invasion of the lesion site by neurites, chiefly peptidergic axons of presumed dorsal root origin, in macrophage-grafted animals compared with controls. In addition, angiogenesis and Schwann cell infiltration were more pronounced after macrophage grafts, providing an increase in laminin, a favorable substrate for axonal regrowth. By using reverse transcription-polymerase chain reaction (RT-PCR), mRNAs for tumor necrosis factor-alpha (TNF-alpha) were detected in the transplanted cells, whereas results were negative for nerve growth factor (NGF), neurotrophin-3 (NT-3), brain-derived neurotrophic factor (BDNF), or acidic fibroblast growth factor (aFGF) and basic fibroblast growth factor (bFGF). Thus, macrophage grafts may represent an interesting strategy to promote axonal regeneration in the CNS. Our study suggests that they may exert their beneficial effects by degrading myelin products, which inhibit axonal regrowth, and by promoting a permissive extracellular matrix containing notably laminin. No evidence for a direct synthesis of neurotrophic factors by the transplanted macrophages was found in this study, but resident glial cells could secrete such factors as a result of stimulation by macrophage-released cytokines.
authors.affiliation: Laboratory of Experimental Neuropathology, University of Liège, Belgium.
authors.forename: R
authors.initials: R
authors.lastname: Franzen
authors.forename: J
authors.initials: J
authors.lastname: Schoenen
authors.forename: P
authors.initials: P
authors.lastname: Leprince
authors.forename: E
authors.initials: E
authors.lastname: Joosten
authors.forename: G
authors.initials: G
authors.lastname: Moonen
authors.forename: D
authors.initials: D
authors.lastname: Martin
mesh_terms: D000818:Animals; D015415:Biomarkers; D015153:Blotting, Western; D002478:Cells, Cultured; D005260:Female; D006133:Growth Substances / Q000096:biosynthesis; D007150:Immunohistochemistry; D017737:Macrophages, Peritoneal / Q000637:transplantation*; D008863:Microspheres; D009474:Neurons / Q000737:chemistry; D016133:Polymerase Chain Reaction / Q000379:methods; D012333:RNA, Messenger / Q000235:genetics; D051381:Rats; D017208:Rats, Wistar; D013119:Spinal Cord Injuries / Q000601:surgery*; D014158:Transcription, Genetic
chemical_list: D015415:Biomarkers; D006133:Growth Substances; D012333:RNA, Messenger