title: Structural characterisation of two hexasaccharides and an octasaccharide from chondroitin sulphate C containing the unusual sequence (4-sulpho)-N-acetylgalactosamine-beta1-4-(2-sulpho)-glucuronic acid-beta1-3-(6-sulpho)-N-acetylgalactosamine.
abstract: The structures of two hexasaccharides and an octasaccharide, isolated from shark cartilage chondroitin sulphate C after partial chondroitin ABC lyase digestion, have been elucidated by disaccharide analysis, liquid secondary-ion mass spectrometry, and 1H/13C NMR spectroscopy. In the case of the octasaccharide, the order of the internal disaccharide units was deduced from a novel one-dimensional TOCSY/ROESY excitation sculpting experiment [Gradwell M. J., Kogelberg, H. & Frenkiel, T. A. (1997) J. Magn. Reson. 124, 267-270] which incorporates two double-pulsed field-gradient spin-echo sequences. The oligosaccharides each contain the unusual motif GalNAc(4SO3-)beta1-4GlcA(2SO3-)beta1-3GalNAc(6SO3++ +-). From the adjoining sequences in the oligosaccharides, it is apparent that this motif occurs interspersed with typical chondroitin sulphate-C disaccharides, GlcAbeta1-3GalNAc(6SO3-). Such differentially sulphated domains along the glycosaminoglycan chains are potential sites for specific recognition processes.
authors.affiliation: The Glycosciences Laboratory, Imperial College School of Medicine, Northwick Park Hospital, Middlesex, England, UK.
authors.forename: W
authors.initials: W
authors.lastname: Chai
authors.forename: A M
authors.initials: AM
authors.lastname: Lawson
authors.forename: M J
authors.initials: MJ
authors.lastname: Gradwell
authors.forename: H
authors.initials: H
authors.lastname: Kogelberg
mesh_terms: D002240:Carbohydrate Sequence; D002809:Chondroitin Sulfates / Q000737:chemistry*; D004187:Disaccharides / Q000737:chemistry; D009682:Magnetic Resonance Spectroscopy; D008969:Molecular Sequence Data; D015394:Molecular Structure; D009844:Oligosaccharides / Q000737:chemistry*; D018629:Spectrometry, Mass, Secondary Ion / Q000379:methods
chemical_list: D004187:Disaccharides; D009844:Oligosaccharides; D002809:Chondroitin Sulfates