title: Application of a standardized coextractive cleanup procedure to routine high-performance liquid chromatography assays of teicoplanin and ganciclovir in plasma.
abstract: Deproteinization of plasma samples with acetonitrile followed by coextracting acetonitrile and lipophilic solutes with chloroform, as already proposed for methotrexate, is stressed as a general sample cleanup procedure for liquid chromatography of highly polar drugs, and was validated for two more applications: teicoplanin and ganciclovir. A dedicated "prevalidation" experimental design was used to assess performances of both assays, including sample preparation. Deviations from linearity were less than 10% over the ranges of 3.1 to 50 mg/l (teicoplanin) and 0.2 to 15 mg/l (ganciclovir), respectively, and limits of quantitation were 0.09 and 0.01 mg/l, respectively. Mean chromatographic measurement R.S.D.s were 4.6% and 1.9%, respectively, with an additional mean cleanup R.S.D. of 2% for both. Mean analyte losses ascribable to cleanup were 6% and 2.5%, respectively from water, and 18% and 12%, respectively from the plasma matrix.
authors.affiliation: Service de Pharmacologie Médicale, Centre Hospitalier Universitaire, Montpellier, France.
authors.forename: M
authors.initials: M
authors.lastname: Cociglio
authors.forename: H
authors.initials: H
authors.lastname: Peyrière
authors.forename: D
authors.initials: D
authors.lastname: Hillaire-Buys
authors.forename: R
authors.initials: R
authors.lastname: Alric
mesh_terms: D000097:Acetonitriles; D000900:Anti-Bacterial Agents / Q000097:blood*; D000998:Antiviral Agents / Q000097:blood*; D002725:Chloroform; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D015774:Ganciclovir / Q000097:blood*; D006801:Humans; D015203:Reproducibility of Results; D012996:Solutions; D017334:Teicoplanin / Q000097:blood*
chemical_list: D000097:Acetonitriles; D000900:Anti-Bacterial Agents; D000998:Antiviral Agents; D012996:Solutions; D017334:Teicoplanin; D002725:Chloroform; D015774:Ganciclovir; C032159:acetonitrile