Is the glutathione S-conjugate pump a flippase?
A hypothesis of the flippase nature of the glutathione S-conjugate transport is presented. Experimental premises for this hypothesis include interaction of glutathione S-conjugates with the membrane, as demonstrated by their effects on membrane fluidity, quenching of 1-(4-trimethylammoniumphenyl)-6-phenyl-1,3,5-hexatriene fluorescence and induction of echinocytosis by 2,4-dinitrophenyl-S-glutathione (DNP-SG). This hypothesis can rationalize, i. a., observations of the enhancement of DNP-SG transport by butanol and stimulation of erythrocyte membrane Mg(2+)-ATPase activity by albumin-coupled DNP-SG.
Sokal
A
A
Department of Molecular Biophysics, University of Lódź, Poland.
Pułaski
L
L
Rychlik
B
B
Fortuniak
A
A
Bartosz
G
G
D020001:1-Butanol / Q000494:pharmacology; D001692:Biological Transport / Q000187:drug effects; D017301:Ca(2+) Mg(2+)-ATPase / Q000097:blood; D002352:Carrier Proteins / Q000097:blood*; D048429:Cell Size / Q000187:drug effects; D004140:Dinitrophenols / Q000097:blood / Q000494:pharmacology; D004910:Erythrocyte Membrane / Q000201:enzymology; D005978:Glutathione / Q000031:analogs & derivatives / Q000097:blood / Q000494:pharmacology; D006801:Humans; D008565:Membrane Proteins / Q000097:blood*; D026901:Membrane Transport Proteins; D049294:Phospholipid Transfer Proteins; D012710:Serum Albumin, Bovine / Q000494:pharmacology
D002352:Carrier Proteins; D004140:Dinitrophenols; D008565:Membrane Proteins; D026901:Membrane Transport Proteins; D049294:Phospholipid Transfer Proteins; C086860:glutathione transporter; C030190:S-(2,4-dinitrophenyl)glutathione; D012710:Serum Albumin, Bovine; D020001:1-Butanol; D017301:Ca(2+) Mg(2+)-ATPase; D005978:Glutathione