title: Nerve growth factor modulates Fc gamma receptor expression on murine macrophage J774A.1 cells.
abstract: We assessed the effect of nerve growth factor (NGF) on Fcgamma receptor (FcgammaR) expression on murine monocyte-macrophage J774A.1 cells. Flow cytometric analysis showed that treatment with various doses of NGF led to a rapid increase of FcgammaRI and FcgammaRII/III expression in a dose-dependent manner. Northern blot analysis with digoxigenin-labeled oligonucleotide probes demonstrated that NGF induced a marked increase in mRNA synthesis of FcgammaRI and FcgammaRII, but not FcgammaRIII. Since pretreatment with K-252a, a tyrosine kinase inhibitor, inhibited the stimulatory effect of NGF completely, the FcgammaR expression augmented by NGF may be mediated through p140trk with a tyrosine kinase domain. These results suggest that NGF may be a novel cytokine that is able to increase FcgammaRI and FcgammaRII expression on macrophages.
authors.affiliation: Department of Veterinary Clinic, Faculty of Agriculture, Tokyo University of Agriculture and Technology, Fuchu, Japan.
authors.forename: Y
authors.initials: Y
authors.lastname: Susaki
authors.forename: A
authors.initials: A
authors.lastname: Tanaka
authors.forename: E
authors.initials: E
authors.lastname: Honda
authors.forename: H
authors.initials: H
authors.lastname: Matsuda
mesh_terms: D000818:Animals; D002460:Cell Line; D005434:Flow Cytometry; D008264:Macrophages / Q000276:immunology; D051379:Mice; D009414:Nerve Growth Factors / Q000494:pharmacology*; D015345:Oligonucleotide Probes; D012333:RNA, Messenger / Q000096:biosynthesis; D017452:Receptors, IgG / Q000096:biosynthesis*; D014158:Transcription, Genetic / Q000187:drug effects; D015854:Up-Regulation / Q000187:drug effects*
chemical_list: D009414:Nerve Growth Factors; D015345:Oligonucleotide Probes; D012333:RNA, Messenger; D017452:Receptors, IgG