[The detection of membrane tumor-associated antigens of Zajdela's hepatoma on the surface of cultured rat cells].
Membrane tumor-associated antigens of the rat hepatoma Zajdela were revealed using immune serum against cell membranes of this hepatoma. By means of SDS-polyacrylamide gel electrophoresis and the following immunoblotting, it was shown that the membrane tumor-associated antigens of hepatoma Zajdela consisted, at least, of 10 components with molecular weights near 22, 25, 31, 33, 42, 46, 56, 65, 96 and 180 kDa. Specific binding of the immune serum with antigens of cultured hepatomas 27 and HTC cell membranes, of myogenic cells and rat kidney fibroblasts may be suggestive of the expression of some tumor-associated hepatoma Zajdela antigens on the surface of these cultured cells.
Teriukova
N P
NP
Institute of Cytology, Russian Academy of Sciences, St. Petersburg.
Tiuriaeva
I I
II
Grandilevskaia
A B
AB
Ivanov
V A
VA
D000818:Animals; D000951:Antigens, Neoplasm / Q000032:analysis*; D000954:Antigens, Surface / Q000032:analysis; D002460:Cell Line; D002478:Cells, Cultured; D004591:Electrophoresis, Polyacrylamide Gel; D005347:Fibroblasts / Q000276:immunology; D019084:Fluorescent Antibody Technique, Indirect; D015151:Immunoblotting; D007668:Kidney; D008114:Liver Neoplasms, Experimental / Q000276:immunology*; D008970:Molecular Weight; D051381:Rats; D014407:Tumor Cells, Cultured
D000951:Antigens, Neoplasm; D000954:Antigens, Surface
Vyiavlenie membrannykh opukholeassotsiirovannykh antigenov gepatomy Zaĭdela na poverkhnosti kul'tiviruemykh kletok krys.