Typing of the short tandem repeat D8S347 locus with different fluorescence markers.
The short tandem repeat (STR) locus D8S347 was analyzed by capillary electrophoresis. Sequencing data and a population study of 203 individuals from a southwestern German population are presented. We detected 12 different alleles, 340-388 bp in length, and found 40 different genotypes. The heterozygosity index was 85.7%. Futhermore, we investigated the consequences of different fluorescent dyes, namely 6-FAM, HEX, and ROX, on the ABI-calculated fragment sizes of defined (i.e., sequenced) alleles (348-376 bp in length). 6-FAM-labeled fragments appear to be smaller than the corresponding HEX- or ROX-labeled fragments. On average, 6-FAM-labeled fragments differ by 3.52 bp from the sequencing data, HEX-labeled ones by 2.04 bp, and ROX-labeled ones by 1.42 bp. Generally, small alleles differ less from the expected sequencing data than larger ones.
Pöltl
R
R
Institut für Anthropologie und Humangenetik, Universität Tübingen, Germany. roger.poeltl@uni-tuebingen.de
Luckenbach
C
C
Fimmers
R
R
Ritter
H
H
D002874:Chromosome Mapping; D002898:Chromosomes, Human, Pair 8; D053938:DNA Fragmentation; D005456:Fluorescent Dyes; D005787:Gene Frequency; D005838:Genotype; D006801:Humans; D018895:Microsatellite Repeats
D005456:Fluorescent Dyes