A female-specific cDNA sequence of Schistosoma mansoni encoding an amidase that is expressed in the gastrodermis.
A female-specifically expressed cDNA clone was obtained by screening of a subtractive cDNA library enriched for RNA from Schistosoma mansoni females. The deduced protein shows significant homology to a class of enzymes functioning as amidases. Northern blots revealed a transcript of 4.0 kb which is absent in larval stages, but is expressed in adult female worms. By in situ hybridization, the expression site of the gene was exclusively localized in the gastrodermis of female schistosomes. This is the first report of a female-specifically transcribed sequence of S. mansoni that is not expressed in the reproductive organs.
Schüssler
P
P
Institute for Genetics, Heinrich-Heine-University, Düsseldorf, Germany.
Köhrer
K
K
Finken-Eigen
M
M
Michel
A
A
Grevelding
C G
CG
Kunz
W
W
D000818:Animals; D001483:Base Sequence; D015152:Blotting, Northern / Q000379:methods; D015139:Blotting, Southern / Q000379:methods; D018076:DNA, Complementary / Q000737:chemistry*; D018509:DNA, Helminth / Q000737:chemistry*; D005260:Female; D017403:In Situ Hybridization / Q000379:methods; D008297:Male; D008969:Molecular Sequence Data; D018510:RNA, Helminth / Q000302:isolation & purification; D012550:Schistosoma mansoni / Q000235:genetics*; D012737:Sex Factors
D018076:DNA, Complementary; D018509:DNA, Helminth; D018510:RNA, Helminth