title: Manipulation and molecular resolution of a phosphatidylcholine-supported planar bilayer by atomic force microscopy.
abstract: The morphology of supported planar bilayers has been investigated below phase transition temperature by atomic force microscopy in contact and tapping mode. The bilayers were formed by the vesicle-spreading technique. In contact mode at low scanning forces of about 1 nN true molecular resolution could be achieved for supported phosphatidylcholine bilayers. The resolution was confirmed by experiments that captured the location, average area of individual lipid headgroups and the manipulation of the bilayer surface. Repeated scanning in contact mode shifted the random topology of the surface consecutively to a striped pattern. Height profiles of defect-containing bilayers were analyzed. The shape of the defects became smooth by repeated scanning. The height profiles allowed the estimation of the indentation of the tip into the surface-adsorbed membrane. In tapping mode a disordered pattern of headgroups became visible. Our morphological data at molecular resolution suggest that the native arrangement of the choline head-groups is disordered, free of large packing defects and becomes ordered in Schallamach waves by scanning in contact mode.
authors.affiliation: Becton Dickinson, Heidelberg, Germany.
authors.forename: M
authors.initials: M
authors.lastname: Beckmann
authors.forename: P
authors.initials: P
authors.lastname: Nollert
authors.forename: H A
authors.initials: HA
authors.lastname: Kolb
mesh_terms: D008051:Lipid Bilayers / Q000737:chemistry; D018625:Microscopy, Atomic Force; D010713:Phosphatidylcholines / Q000737:chemistry
chemical_list: D008051:Lipid Bilayers; D010713:Phosphatidylcholines