title: Gene organization and sequence determination of the two botulinum neurotoxin gene clusters in Clostridium botulinum type A(B) strain NCTC 2916.
abstract: The gene organization and nucleotide sequence of the type A and B BoNT-gene clusters in Clostridium botulinum strain NCTC 2916 were studied. The aim was to clarify the organization of genes within C. botulinum type A strains possessing an unexpressed BoNT/B gene. The BoNT/A-gene cluster includes genes encoding BoNT, NTNH and a part of P-47 (the gene for this protein was reported in strains of C. botulinum types E and F). Clustered with the silent BoNT/B gene were genes encoding NTNH, P-21 and HA-33. Sequencing analysis of the NTNHs revealed the presence of 471 amino acids identical in the type B and A gene clusters. This gene organization contrasts markedly with the purported organization in strain NCTC 2916 described by Henderson et al. (FEMS Microbiol. Lett. 140, 151-158). In type A(B) strain NCTC 2916, the neurotoxin gene is of type BoNT/A1 within a gene cluster that has identical organization to that found in BoNT/A2 type strains; these observations may be significant in establishing the origin of the BoNT-gene cluster.
authors.affiliation: Institute of Food Research, Earley Gate, Whiteknights Road, Reading, Berkshire, RG6 6BZ, UK.
authors.forename: M
authors.initials: M
authors.lastname: Rodríguez Jovita
authors.forename: M D
authors.initials: MD
authors.lastname: Collins
authors.forename: A K
authors.initials: AK
authors.lastname: East
mesh_terms: D001905:Botulinum Toxins / Q000235:genetics*; D019274:Botulinum Toxins, Type A / Q000235:genetics*; D003014:Clostridium botulinum / Q000235:genetics*; D004269:DNA, Bacterial / Q000032:analysis; D005798:Genes, Bacterial / Q000235:genetics*; D008969:Molecular Sequence Data; D005810:Multigene Family / Q000235:genetics*; D016133:Polymerase Chain Reaction
chemical_list: D004269:DNA, Bacterial; C096323:rimabotulinumtoxinB; D001905:Botulinum Toxins; D019274:Botulinum Toxins, Type A