Directed termination PCR: a one-step approach to mutation detection.
We describe a novel PCR-based method that allows the generation of nested termination fragments by integrating both selective DNA amplification and directed chain termination into a single PCR reaction. These termination fragments can be examined for sequence variation in either denaturing or non-denaturing polyacrylamide gels. This method provides a one-step and highly effective approach for the detection of both insertions/deletions and single base pair substitutions in sequences up to 1 kb in length.
Chen
J
J
Department of Zoology, University of Guelph, Guelph, Ontario N1G 2W1, Canada. jjchen@uoguelph.ca
Hebert
P D
PD
D000180:Acrylic Resins; D000818:Animals; D004252:DNA Mutational Analysis / Q000379:methods*; D004272:DNA, Mitochondrial / Q000235:genetics / Q000302:isolation & purification; D019398:Genes, BRCA1; D006801:Humans; D007059:Ictaluridae / Q000235:genetics; D007231:Infant, Newborn; D009154:Mutation; D009691:Nucleic Acid Denaturation; D017354:Point Mutation; D016133:Polymerase Chain Reaction / Q000379:methods*; D018807:Polymorphism, Single-Stranded Conformational; D017384:Sequence Deletion
D000180:Acrylic Resins; D004272:DNA, Mitochondrial; C016680:polyacrylamide gels