Comparison of antiproliferative effects of atrial natriuretic peptide and transforming growth factor beta on rabbit kidney proximal tubule cells.
The effects of atrial natriuretic peptide (ANP) and transforming growth factor beta (TGF beta) on cell proliferation were investigated in rabbit kidney proximal tubule cells. At 48 h, each agonist inhibited cell growth dose dependently. Moreover, the antiproliferative effect of both factors together was greater than that of each factor alone. However, coincubation of a high concentration (100 nM) of ANP and epidermal growth factor (EGF) was found to induce cell hypertrophy. This hypertrophic effect induced by ANP in the presence of EGF was mimicked by 8-BrcGMP but not by various cAMP analogues, at 100 microM, and was independent of the tyrosine kinase inhibitor genistein (10 microM). However, it was inhibited by 100 pM TGF beta. These in vitro results suggest that the antiproliferative effects of ANP and TGF beta may be part of a mechanism to modulate the hyperplastic effect of EGF in the renal proximal tubule during compensatory kidney growth.
García-Ocaña
A
A
Laboratorio de la Unidad Metabólica, Fundación Jiménez Díaz, Madrid, Spain.
Peñaranda
C
C
Esbrit
P
P
D000704:Analysis of Variance; D000818:Animals; D009320:Atrial Natriuretic Factor / Q000494:pharmacology*; D002455:Cell Division; D004305:Dose-Response Relationship, Drug; D007687:Kidney Tubules, Proximal / Q000166:cytology* / Q000187:drug effects*; D011817:Rabbits; D016212:Transforming Growth Factor beta / Q000494:pharmacology*
D016212:Transforming Growth Factor beta; D009320:Atrial Natriuretic Factor