Protective activity of Borrelia duttonii-specific immunoglobulin subclasses in mice.
To analyse the immune response of mice to Borrelia duttonii infection, BALB/c mice were inoculated intraperitoneally with B. duttonii strain 406K, and the titres of B. duttonii-specific immunoglobulins - IgM, IgG1, IgG2a, IgG2b and IgG3 - were determined by ELISA. IgM antibodies appeared first, followed by IgG2a and IgG3, and then IgG1 and IgG2b. The protective activity of individual classes and subclasses of B. duttonii-specific immunoglobulins was then determined by passive immunisation of BALB/c mice with immunoglobulin preparations purified by affinity chromatography. The mice were then challenged by intraperitoneal inoculation of B. duttonii. The study demonstrated that B. duttonii-specific IgM and IgG3 protected against the development of spirochaetaemia and death after borrelial infection, whereas B. duttonii-specific IgG1, IgG2a and IgG2b had low protective activities.
Yokota
M
M
Department of Microbiology, Yamaguchi University School of Medicine, Ube, Japan.
Morshed
M G
MG
Nakazawa
T
T
Konishi
H
H
D000818:Animals; D000907:Antibodies, Bacterial / Q000145:classification / Q000276:immunology / Q000302:isolation & purification; D000917:Antibody Formation / Q000276:immunology; D001898:Borrelia / Q000276:immunology*; D001899:Borrelia Infections / Q000276:immunology* / Q000382:microbiology; D005260:Female; D007116:Immunization, Passive; D007074:Immunoglobulin G / Q000008:administration & dosage / Q000276:immunology; D007075:Immunoglobulin M / Q000008:administration & dosage / Q000276:immunology; D007136:Immunoglobulins / Q000145:classification / Q000276:immunology* / Q000302:isolation & purification; D051379:Mice; D008807:Mice, Inbred BALB C; D013997:Time Factors
D000907:Antibodies, Bacterial; D007074:Immunoglobulin G; D007075:Immunoglobulin M; D007136:Immunoglobulins