Circulating immune complexes from HIV-1+ patients induces apoptosis on normal lymphocytes.
Isolated immune complexes from sera of 49 out of 67 human immunodeficiency virus-1-positive (HIV-1+) patients (CIC-HIV+), composed of anti-HIV-HIV-Ag, could induce apoptosis on normal phytohaemagglutinin (PHA)-activated lymphocytes. DNA degradation was detected by propidium iodide staining. This activity is directed against CD4+ lymphocytes as demonstrated by double binding of CIC-HIV+ and anti-CD4 on apoptosis cells. Expression of Fas antigen is prior to apoptotic phenomena. CIC-HIV+ apoptosis inducers belong mainly to asymptomatic HIV-infected patients, indicating that immune complexes from these patients can destroy CD4+ lymphocytes.
Aceituno
E
E
Department of Immunology, Fundación Jiménez Díaz, Madrid, Spain.
Castañón
S
S
Jiménez
C
C
Subirá
D
D
De Górgolas
M
M
Fernández-Guerrero
M
M
Ortíz
F
F
García
R
R
D000328:Adult; D000936:Antigen-Antibody Complex / Q000097:blood*; D017209:Apoptosis / Q000276:immunology*; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology*; D018929:Cell Culture Techniques; D005260:Female; D006679:HIV Seropositivity / Q000276:immunology*; D015497:HIV-1 / Q000276:immunology*; D006801:Humans; D008213:Lymphocyte Activation / Q000276:immunology; D008297:Male; D019014:fas Receptor / Q000097:blood
D000936:Antigen-Antibody Complex; D019014:fas Receptor