A sustained increase of cytosolic Ca2+ in gammadelta T cells triggered by co-stimulation via TCR/CD3 and LFA-1.
We previously reported that co-stimulation with LFA-1 triggered apoptosis in gammadelta T cells but not in alphabeta T cells after TCR engagement. We extended our earlier study on TCR/LFA-1 triggered apoptosis to two autoreactive TCR gammadelta and TCR alphabeta T cell clones, which were derived from syngeneic mixed lymphocyte culture of BALB/c mice. A gammadelta T cell clone, KM1, expressed the Vgamma4 and Vdelta5 genes and CD4-CD8-CD45RB+ phenotype; and an alphabeta T cell clone, BASL1.1, expressed Vbeta6 and CD4+CD8-CD45RB+. Both clones produced Th-1-type cytokines in response to syngeneic BALB/c stimulator cells. KM1 underwent apoptosis upon stimulation with immobilized anti-CD3/LFA-1 mAbs, whereas BASL1.1 could proliferate successfully in response to stimulation with the immobilized mAbs. BASL1.1 was able to down-regulate the increased cytosolic Ca2+ after the simultaneous stimulation, but KM1 exhibited a sustained increase of cytosolic Ca2+ after stimulation via CD3 and LFA-1. Similar results with respect to the kinetics of cytosolic Ca2+ were obtained with normal heterogeneous gammadelta and alphabeta T cell populations after co-stimulation via CD3 and LFA-1. Our results suggested that persistently high levels of cytosolic Ca2+ might be related to apoptosis in gammadelta T cell clone triggered by co-stimulation via CD3 and LFA-1.
Kobayashi
N
N
Laboratory of Host Defense, Research Institute for Disease Mechanism and Control, Nagoya University School of Medicine, Japan.
Hiromatsu
K
K
Matsuzaki
G
G
Harada
M
M
Matsumoto
Y
Y
Nomoto
K
K
Yoshikai
Y
Y
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D017209:Apoptosis; D017252:CD3 Complex / Q000276:immunology / Q000378:metabolism*; D002118:Calcium / Q000378:metabolism*; D002455:Cell Division; D002460:Cell Line; D003432:Cross-Linking Reagents; D003600:Cytosol / Q000378:metabolism; D005260:Female; D016169:Lymphocyte Function-Associated Antigen-1 / Q000276:immunology / Q000378:metabolism*; D051379:Mice; D008807:Mice, Inbred BALB C; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000096:biosynthesis; D016693:Receptors, Antigen, T-Cell, alpha-beta / Q000378:metabolism; D016692:Receptors, Antigen, T-Cell, gamma-delta / Q000378:metabolism*
D000911:Antibodies, Monoclonal; D017252:CD3 Complex; D003432:Cross-Linking Reagents; D016169:Lymphocyte Function-Associated Antigen-1; D019253:Proto-Oncogene Proteins c-bcl-2; D016693:Receptors, Antigen, T-Cell, alpha-beta; D016692:Receptors, Antigen, T-Cell, gamma-delta; D002118:Calcium