Silver staining as a new banding technique to identify aphid chromosomes.
The karyotype of the aphid Aphis sambuci has been studied by means of C-banding, fluorochrome staining and AgNO3 staining. Heterochromatic areas are confined exclusively to the X chromosomes. Interestingly, silver staining produces a clear-cut longitudinal differentiation of all chromosomes, which allows reliable identification of homologues.
Manicardi
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GC
Dipartimento di Biologia Animale, Università di Modena, Italy. bianchi@c220.unimo.it
Bizzaro
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Mandrioli
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Bianchi
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D000818:Animals; D001042:Aphids / Q000235:genetics*; D002871:Chromosome Banding / Q000379:methods*; D005260:Female; D005456:Fluorescent Dyes; D007211:Indoles; D007621:Karyotyping; D016622:Silver Staining / Q000379:methods*; D014960:X Chromosome
D005456:Fluorescent Dyes; D007211:Indoles; C007293:DAPI