title: Analysis of a Streptomyces coelicolor A3(2) locus containing the nucleoside diphosphate kinase (ndk) and folylpolyglutamate synthetase (folC) genes.
abstract: A 3.6-kb DNA fragment from Streptomyces coelicolor A3(2) with the genes valS probably encoding a valyl-tRNA synthetase, folC encoding folylpolyglutamate synthetase, and ndk encoding a nucleoside diphosphate kinase was analysed. folC and ndk are separated by a small open reading frame of unknown function, orfX. The deduced folC gene product is a protein of 46,677 Da whose sequence is similar to other folylpolyglutamate synthetases and folylpolyglutamate synthetase-dihydrofolate synthetases from both Gram-positive and Gram-negative bacteria. After cloning folC behind the lacZ promoter, the Streptomyces folC complemented a folC mutant of Escherichia coli. An essential function for Streptomyces folC was suggested by the fact that it could not be mutated using a conventional gene disruption technique.
authors.affiliation: Eberhard-Karls-Universität Tübingen, Germany.
authors.forename: A
authors.initials: A
authors.lastname: Burger
authors.forename: B
authors.initials: B
authors.lastname: Brandt
authors.forename: U
authors.initials: U
authors.lastname: Süsstrunk
authors.forename: C J
authors.initials: CJ
authors.lastname: Thompson
authors.forename: W
authors.initials: W
authors.lastname: Wohlleben
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D002874:Chromosome Mapping; D003001:Cloning, Molecular; D005798:Genes, Bacterial; D008969:Molecular Sequence Data; D009701:Nucleoside-Diphosphate Kinase / Q000235:genetics*; D010453:Peptide Synthases / Q000235:genetics*; D013302:Streptomyces / Q000235:genetics* / Q000254:growth & development
chemical_list: D009701:Nucleoside-Diphosphate Kinase; D010453:Peptide Synthases; C023103:folylpolyglutamate synthetase