Role of substance P in experimental allergic conjunctivitis in guinea pigs.
The role of substance P in experimental allergic conjunctivitis induced by egg albumin was investigated with guinea pigs. Increase in vascular permeability of the conjunctiva induced by antigen was significantly inhibited after repeated application of capsaicin. Substance P contents in the conjunctiva of guinea pig were decreased by topical instillation of antigen to the eyes, suggesting that substance P was released from the conjunctiva due to antigen-antibody reaction. Moreover, subconjunctival injection of substance P resulted in a dose-related conjunctivitis, and vascular permeability in the conjunctiva was also increased by substance P. In substance P-induced conjunctivitis, a significant edema was observed in the bulbar and palpebral conjunctiva, but no hyperemia was noted in all instances. Histamine contents of the conjunctiva and tears were not influenced by subconjunctival injection of substance P. However, topical application of antigen and subconjunctival injection of compound 48/80 caused a significant decrease in histamine content, and content of tear was increased by both treatments. An increase in vascular permeability induced by antigen application was significantly inhibited by intravenous injection of FK-888, which is a specific and potent NK1 receptor antagonist. From these results, it is suggested that substance P is responsible for allergic conjunctivitis to some extent, and the conjunctival hyperpermeability induced by substance P occurs through NK1 receptor on the blood vessels, rather than by the direct action on the conjunctival mast cells during allergic conjunctival reactions.
Yamaji
M
M
Department of Pharmacology, Faculty of Pharmaceutical Sciences, Okayama University, Japan.
Takada
M
M
Fujiwara
R
R
Ohishi
H
H
Izushi
K
K
Sugimoto
Y
Y
Kamei
C
C
D000818:Animals; D002199:Capillary Permeability / Q000187:drug effects; D002211:Capsaicin / Q000494:pharmacology; D003228:Conjunctiva / Q000098:blood supply / Q000187:drug effects; D003233:Conjunctivitis, Allergic / Q000209:etiology / Q000473:pathology / Q000517:prevention & control*; D004151:Dipeptides / Q000494:pharmacology; D004195:Disease Models, Animal; D006168:Guinea Pigs; D006632:Histamine / Q000032:analysis; D007211:Indoles / Q000494:pharmacology; D008297:Male; D008407:Mast Cells / Q000187:drug effects / Q000378:metabolism; D010047:Ovalbumin; D015842:Serine Proteinase Inhibitors; D013373:Substance P / Q000008:administration & dosage / Q000494:pharmacology*; D013666:Tears / Q000737:chemistry; D003189:p-Methoxy-N-methylphenethylamine / Q000494:pharmacology
D004151:Dipeptides; D007211:Indoles; D015842:Serine Proteinase Inhibitors; C078949:FK 888; D013373:Substance P; D003189:p-Methoxy-N-methylphenethylamine; D006632:Histamine; D010047:Ovalbumin; D002211:Capsaicin