The spoIIE locus is involved in the Spo0A-dependent switch in the location of FtsZ rings in Bacillus subtilis.
A switch in the location of FtsZ ring structures from medial to polar is one of the earliest morphological indicators of sporulation in Bacillus subtilis. This switch can be artificially caused during vegetative growth by induction of an active form, Sad67, of the transcription regulator, Spo0A (P. A. Levin and R. Losick, Genes Dev. 10:478-488, 1996). We have used immunofluorescence microscopy to show that the switch in FtsZ ring location during vegetative growth caused by Sad67 induction is blocked by a spoIIE deletion mutation. The spoIIE mutation also impaired polar FtsZ ring formation during sporulation. These results suggest that SpoIIE mediates the Spo0A-directed formation of polar FtsZ rings.
Khvorova
A
A
Department of Microbiology and Immunology, Temple University School of Medicine, Philadelphia, Pennsylvania 19140, USA.
Zhang
L
L
Higgins
M L
ML
Piggot
P J
PJ
D001412:Bacillus subtilis / Q000737:chemistry / Q000235:genetics* / Q000254:growth & development / Q000502:physiology*; D001426:Bacterial Proteins / Q000032:analysis* / Q000235:genetics* / Q000502:physiology*; D003470:Culture Media; D003598:Cytoskeletal Proteins; D015964:Gene Expression Regulation, Bacterial; D005798:Genes, Bacterial; D008856:Microscopy, Fluorescence; D017384:Sequence Deletion; D012808:Sigma Factor; D013171:Spores, Bacterial / Q000502:physiology; D014157:Transcription Factors / Q000502:physiology*
D001426:Bacterial Proteins; D003470:Culture Media; D003598:Cytoskeletal Proteins; C071525:FtsZ protein, Bacteria; D012808:Sigma Factor; C080950:Spo0A protein, Bacillus subtilis; D014157:Transcription Factors; C092468:spoIIR protein, Bacillus subtilis; C030069:spore-specific proteins, Bacillus