Chronic n-3 polyunsaturated fatty acid diet-deficiency acts on dopamine metabolism in the rat frontal cortex: a microdialysis study.
The effects of alpha-linolenic acid diet deficiency on rat dopaminergic metabolism were investigated in the frontal cortex of male 2-3 month-old rats using the microdialysis method. Increased basal levels of dopamine metabolites were observed in the frontal cortex of awake deficient rats, without modification of dopamine levels. Moreover, using KCl perfusion which releases newly synthesized dopamine, no difference was observed in anaesthetized deficient rats versus control rats. In addition, a decrease in dopamine release was observed in anaesthetized deficient rats versus control rats after tyramine stimulation, which is known to induce release of dopamine from vesicular stores. A working model is proposed which suggests that a chronic n-3 polyunsaturated fatty acids (PUFA) deficiency may lead to modifications in the internalization of dopamine in the storage pool in the frontal cortex.
Zimmer
L
L
INSERM U316, Laboratoire de Biophysique Médicale et Pharmaceutique, Faculté de Pharmacie, Tours, France.
Hembert
S
S
Durand
G
G
Breton
P
P
Guilloteau
D
D
Besnard
J C
JC
Chalon
S
S
D000704:Analysis of Variance; D000818:Animals; D018752:Diet, Fat-Restricted / Q000009:adverse effects*; D004042:Dietary Fats, Unsaturated / Q000008:administration & dosage*; D004298:Dopamine / Q000378:metabolism*; D005260:Female; D005625:Frontal Lobe / Q000187:drug effects / Q000378:metabolism*; D008297:Male; D017551:Microdialysis / Q000379:methods; D010477:Perfusion; D051381:Rats; D017208:Rats, Wistar; D013238:Stereotaxic Techniques; D009435:Synaptic Transmission / Q000187:drug effects; D014439:Tyramine / Q000008:administration & dosage; D017962:alpha-Linolenic Acid / Q000008:administration & dosage / Q000172:deficiency*
D004042:Dietary Fats, Unsaturated; D017962:alpha-Linolenic Acid; D004298:Dopamine; D014439:Tyramine