Reversed-phase high-performance liquid chromatographic assay methods for the analysis of a range of penicillins in in vitro permeation studies.
Simple, rapid and selective HPLC methods have been developed for the analysis of amoxycillin, ampicillin, epicillin, mecillinam and propicillin. A Hypersil ODS 5 microm (150x4.6 mm I.D.) column was used with mobile phases containing aqueous phosphate buffers, pH 3-4.6 and either methanol or acetonitrile as the organic modifier. Samples were detected by their optimal UV absorption (210-230 nm). The lower limits of quantitation of the compounds (100 microl injection volume) were 0.1 microg/ml. The assays were linear in the range of 0.1-100 microg/ml with r2 values greater than 0.99. The methods have been applied successfully for the measurement of the flux of the compounds across Caco-2 cells monolayers.
Erah
P O
PO
Department of Pharmaceutical Sciences, University of Nottingham, UK.
Barrett
D A
DA
Shaw
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PN
D018938:Caco-2 Cells; D002851:Chromatography, High Pressure Liquid; D006801:Humans; D008566:Membranes; D010406:Penicillins / Q000032:analysis* / Q000378:metabolism; D010539:Permeability; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013056:Spectrophotometry, Ultraviolet
D010406:Penicillins