title: Isolation of wild Xanthomonas strains from agricultural produce, their characterization and potential related to polysaccharide production.
abstract: One hundred fifty wild strains of Xanthomonas were isolated on five selective and nonselective media. The comparative effectivity of the five media has been discussed. Fifteen polysaccharide-producing strains shortlisted were identified up to species level. These were studied for polysaccharide production in shake flasks using YM media. The highest final yield of 21.3 g/L of the amount of carbon source supplied has been obtained in the optimum medium in shake-flask experiments from Xanthomonas campestris (ICa-125 strain isolated from cabbage. Rheological properties of the exocellular polysaccharide (EPS) have been compared with standard xanthan. EPS from X. campestris ICa-125 was found to be superior with respect to heat stability and effect of electrolytes as compared to standard xanthan.
authors.affiliation: Food and Fermentation Technology Division, University Department of Chemical Technology, University of Mumbai, Matunga, India.
authors.forename: M D
authors.initials: MD
authors.lastname: Gupte
authors.forename: M Y
authors.initials: MY
authors.lastname: Kamat
mesh_terms: D001431:Bacteriological Techniques; D003470:Culture Media; D005516:Food Microbiology; D007194:India; D011135:Polysaccharides, Bacterial / Q000096:biosynthesis* / Q000737:chemistry / Q000302:isolation & purification; D014675:Vegetables / Q000382:microbiology*; D014783:Viscosity; D014974:Xanthomonas / Q000302:isolation & purification* / Q000378:metabolism
chemical_list: D003470:Culture Media; D011135:Polysaccharides, Bacterial; C002563:xanthan gum