title: Prevalence and characterization of perinuclear anti-neutrophil cytoplasmic antibodies (P-ANCA) directed against HMG1 and HMG2 in ulcerative colitis (UC).
abstract: In a previous study, we reported that the high mobility group (HMG) non-histone chromosomal proteins HMG1 and HMG2 were novel target antigens of P-ANCA. In this study, we determined the immunodiagnostic value of anti-HMG1/HMG2 antibodies in patients with UC. Sixty sera from patients with UC were tested for reactivity with HMG1 and HMG2 by means of ELISA. Anti-HMG1 antibody was detected in 32% of patients (40% of P-ANCA+ patients). Anti-HMG2 antibody was detected in 33% (40% of P-ANCA+ patients). Thirty-five percent of sera were positive for antibody to either HMG1 or HMG2 (43% of P-ANCA+ patients). P-ANCA+ patients expressed anti-HMG1/HMG2 antibodies with significantly greater frequency compared with P-ANCA- patients. Furthermore, the anti-HMG1/HMG2 antibodies were significantly related to disease activity in UC. Sixteen of the 18 UC patients, who had high titres of anti-HMG1 or -HMG2 antibody during the active phase, showed lower titres in the inactive phase. Anti-HMG1/HMG2 antibodies appear to be useful as a marker for disease activity in UC.
authors.affiliation: Department of Medicine and Clinical Science, Kyoto University Graduate School of Medicine, Japan.
authors.forename: J
authors.initials: J
authors.lastname: Sobajima
authors.forename: S
authors.initials: S
authors.lastname: Ozaki
authors.forename: H
authors.initials: H
authors.lastname: Uesugi
authors.forename: F
authors.initials: F
authors.lastname: Osakada
authors.forename: H
authors.initials: H
authors.lastname: Shirakawa
authors.forename: M
authors.initials: M
authors.lastname: Yoshida
authors.forename: K
authors.initials: K
authors.lastname: Nakao
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000818:Animals; D000906:Antibodies / Q000032:analysis / Q000097:blood; D019268:Antibodies, Antineutrophil Cytoplasmic / Q000097:blood / Q000276:immunology*; D015153:Blotting, Western; D056649:Cathepsin G; D002403:Cathepsins / Q000276:immunology; D002648:Child; D003093:Colitis, Ulcerative / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006609:High Mobility Group Proteins / Q000276:immunology*; D006801:Humans; D007781:Lactoferrin / Q000276:immunology; D008297:Male; D008875:Middle Aged; D012697:Serine Endopeptidases; D013552:Swine
chemical_list: D000906:Antibodies; D019268:Antibodies, Antineutrophil Cytoplasmic; D006609:High Mobility Group Proteins; D002403:Cathepsins; D007781:Lactoferrin; D012697:Serine Endopeptidases; C534094:CTSG protein, human; D056649:Cathepsin G