title: SASP (small, acid-soluble spore proteins) and spore properties in Bacillus thuringiensis israelensis and Bacillus sphaericus.
abstract: Entomopathogenic bacilli B. thuringiensis israelensis (Bti) and B. sphaericus (Bf) exhibit low survival on field application. It was previously shown that their spores are very sensitive to different stress effectors (heat, UV light) and especially to osmotic variations. Since SASP (Small, Acid-Soluble Spore Proteins), alpha/beta and gamma type, are involved in spore tolerance to heat, UV light, peroxide, and salt, they were analyzed in Bti and Bf. The molecular weight, migration pattern and amino acid composition of different SASP were determined and compared with other bacilli, in particular to B. subtilis. A relation between spore osmotolerance, SASP content, and amino acid composition was shown. In addition, the absence of gamma SASP in Bti and Bf is discussed.
authors.affiliation: Departamento de Química Biológica, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Ciudad Universitaria, Pabellón II (1428), Buenos Aires, Argentina.
authors.forename: A
authors.initials: A
authors.lastname: Cucchi
authors.forename: C
authors.initials: C
authors.lastname: Sanchez de Rivas
mesh_terms: D001407:Bacillus / Q000187:drug effects / Q000378:metabolism* / Q000528:radiation effects; D001413:Bacillus thuringiensis / Q000187:drug effects / Q000378:metabolism* / Q000528:radiation effects; D001426:Bacterial Proteins / Q000187:drug effects / Q000378:metabolism* / Q000528:radiation effects; D009997:Osmotic Pressure / Q000187:drug effects; D010545:Peroxides / Q000494:pharmacology; D012965:Sodium Chloride / Q000494:pharmacology; D013171:Spores, Bacterial / Q000187:drug effects / Q000378:metabolism* / Q000528:radiation effects; D013696:Temperature; D014466:Ultraviolet Rays
chemical_list: D001426:Bacterial Proteins; D010545:Peroxides; D012965:Sodium Chloride