Fumarate permeation in normal and acidotic rat kidney mitochondria: fumarate/malate and fumarate/aspartate translocators.
In order to gain some insight into the fate of fumarate synthesised in the cytosol in the purine nucleotide cycle and in amino acid catabolism, the capability of both rat kidney mitochondria and acidotic rat kidney mitochondria to take up either externally synthesised, via adenylsuccinate lyase, or added fumarate in exchange with intramitochondrial malate or aspartate was tested by means of both spectrophotometric and isotopic techniques. The appearance of either malate or aspartate caused by the presence of fumarate was revealed outside normal and acidotic mitochondria by using specific substrate detecting systems. Consistently, externally added fumarate was found to cause efflux of either [14C]-malate or [14C]-aspartate from loaded mitochondria. The occurrence in rat kidney mitochondria of two separate translocators, i.e., fumarate/malate and fumarate/aspartate carriers, is shown in the light of saturation kinetics and the different inhibitor sensitivity. The fumarate/aspartate antiporters found in normal and acidotic mitochondria appear to differ from each other.
Atlante
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Centro di Studio sui Mitocondri e Metabolismo Energetico, CNR, Bari, Italy.
Gagliardi
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Passarella
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D000138:Acidosis / Q000378:metabolism*; D000264:Adenylosuccinate Lyase / Q000378:metabolism; D000818:Animals; D001224:Aspartic Acid / Q000378:metabolism*; D001692:Biological Transport; D002352:Carrier Proteins / Q000378:metabolism; D005650:Fumarates / Q000378:metabolism* / Q000494:pharmacology; D007668:Kidney / Q000648:ultrastructure*; D008293:Malates / Q000378:metabolism*; D008297:Male; D008928:Mitochondria / Q000187:drug effects / Q000378:metabolism*; D009243:NAD / Q000378:metabolism; D009249:NADP / Q000378:metabolism; D010071:Oxaloacetates / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar
D002352:Carrier Proteins; D005650:Fumarates; D008293:Malates; D010071:Oxaloacetates; D009243:NAD; D001224:Aspartic Acid; D009249:NADP; C030298:malic acid; C032005:fumaric acid; D000264:Adenylosuccinate Lyase