Physical and genetic map of the genome of Staphylococcus carnosus TM300.
A genome map of Staphylococcus carnosus TM300, an important micro-organism in the food industry and long used as a starter culture, was constructed by pulsed-field gel electrophoresis of DNA fragments obtained after digestion with NotI, SfiI and ApaI. The size of the chromosome was estimated to be 2590 kb. The fragments were assembled into a physical map using a combination of complementary methods including multiple and partial digests of genomic DNA, hybridization with homologous gene probes, and cross-Southern hybridization. Fifteen genes or gene clusters were positioned on the physical map by Southern hybridization analysis. The map provides a basis for further analysis of the S. carnosus chromosome.
Wagner
Elke
E
Lehrstuhl für Mikrobielle Genetik, Universität Tübingen, 72076 Tübingen, Germany.
Doskar
Jirí
J
Department of Genetics and Molecular Biology, Faculty of Science, Masaryk University, 61137 Brno, Czech Republic.
Götz
Friedrich
F
Lehrstuhl für Mikrobielle Genetik, Universität Tübingen, 72076 Tübingen, Germany.
D002874:Chromosome Mapping; D015342:DNA Probes; D004262:DNA Restriction Enzymes / Q000378:metabolism; D004269:DNA, Bacterial / Q000032:analysis / Q000235:genetics; D016521:Electrophoresis, Gel, Pulsed-Field; D005516:Food Microbiology; D005798:Genes, Bacterial; D016680:Genome, Bacterial; D005810:Multigene Family; D009693:Nucleic Acid Hybridization; D013210:Staphylococcus / Q000235:genetics*
D015342:DNA Probes; D004269:DNA, Bacterial; D004262:DNA Restriction Enzymes