title: Plus/minus screening of rabbit corneal endothelial cDNA library.
abstract: Plus/minus screening of the rabbit corneal cDNA library was performed using corneal and iris RNA as probes. Thirteen clones were isolated: three ferritin H-chains, a NADH-ubiquinone oxidoreductase B22 subunit, an alpha 1 type VIII collagen, a 25 KDa FKBP-506 binding protein (FKBP25), a thrombospondin 2, and six unknown clones. Although proteins translocated from these isolated mRNA are not corneal specific, they play an important role in the cornea. None of the isolated known mRNAs maps to chromosome 1, 16, or 20. These clones, thrombospondin excepted, were not observed in the high frequency clones in the profile of the aortic endothelial cDNA library.
authors.affiliation: Department of Ophthalmology, Juntendo University School of Medicine, Tokyo, Japan.
authors.forename: Y
authors.initials: Y
authors.lastname: Hotta
authors.forename: H
authors.initials: H
authors.lastname: Kitagawa
authors.forename: K
authors.initials: K
authors.lastname: Fujiki
authors.forename: T
authors.initials: T
authors.lastname: Fujimaki
authors.forename: H
authors.initials: H
authors.lastname: Ohnuki
authors.forename: H
authors.initials: H
authors.lastname: Sakuma
authors.forename: F
authors.initials: F
authors.lastname: Iwata
authors.forename: M
authors.initials: M
authors.lastname: Watanabe
authors.forename: K
authors.initials: K
authors.lastname: Nakayasu
authors.forename: A
authors.initials: A
authors.lastname: Kanai
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002352:Carrier Proteins / Q000235:genetics; D002417:Cattle; D015815:Cell Adhesion Molecules / Q000235:genetics; D002999:Clone Cells; D003094:Collagen / Q000235:genetics; D018076:DNA, Complementary / Q000032:analysis*; D004587:Electrophoresis, Agar Gel; D004728:Endothelium, Corneal; D005136:Eye Proteins / Q000235:genetics*; D005293:Ferritins / Q000235:genetics; D015723:Gene Library; D006801:Humans; D008969:Molecular Sequence Data; D016660:NAD(P)H Dehydrogenase (Quinone) / Q000235:genetics; D011817:Rabbits; D017422:Sequence Analysis, DNA; D022021:Tacrolimus Binding Proteins; D019699:Thrombospondins / Q000235:genetics; D000099262:Thrombospondin 2
chemical_list: D002352:Carrier Proteins; D015815:Cell Adhesion Molecules; D003094:Collagen; D018076:DNA, Complementary; D005136:Eye Proteins; D005293:Ferritins; D016660:NAD(P)H Dehydrogenase (Quinone); D022021:Tacrolimus Binding Proteins; D019699:Thrombospondins; D000099262:Thrombospondin 2; C074856:FKBP3 protein, human