New murine monoclonal antibodies directed against glycophorins C and D, have anti-Ge2 specificity.
OBJECTIVE
The Gerbich blood group system comprises three high-incidence antigens which have been localized on glycophorins C (GPC) and D (GPD) molecules. Murine monoclonal antibodies (mAbs) directed against Ge4 and Ge3 epitopes have already been obtained. We describe two murine mAbs (ZI51.17 and J6) with Ge2 specificity.


METHODS
The mAbs were investigated by serological methods and immunoblotting, with common, Ge-negative and enzyme-modified RBCs.


RESULTS
Both mAbs behaved like anti-Ge2 in serological tests, but bound to GPC and GPD on immunoblots. ZI51.17 did not recognize the GPCYus or GPCGe variants, whereas J6 revealed a 25-kD component from Ge:-2,-3,4 membranes, which is identical to the GPCGe molecule.


CONCLUSIONS
These two mAbs are directed to slightly different epitopes. The ZI51.17 and J6 epitopes are common to GPC and GPD, thus distinct from the Ge2 antigen defined by human alloantibodies.
Janvier
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Etablissement de Transfusion Sanguine de l'Assistance Publique, Hôpitaux de Paris, Site St-Louis, France.
Veaux
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Benbunan
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D000372:Agglutination Tests; D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D001789:Blood Group Antigens / Q000276:immunology*; D000939:Epitopes / Q000097:blood / Q000235:genetics; D005091:Exons; D006021:Glycophorins / Q000235:genetics / Q000276:immunology* / Q000378:metabolism; D006801:Humans; D015151:Immunoblotting; D051379:Mice; D008807:Mice, Inbred BALB C / Q000276:immunology*; D011485:Protein Binding
D000911:Antibodies, Monoclonal; D001789:Blood Group Antigens; D000939:Epitopes; D006021:Glycophorins