title: Regional and molecular separation of the four bioactivities of 'tribulin'.
abstract: The endocoid tribulin has four known bioactivities: monoamine oxidase A and B inhibitory activities (MAO-AI and MAO-BI) and peripheral and central benzodiazepine receptor binding inhibitory activities (PBR-I and CBR-I). Analysis of the four bioactivities in rat tissue reveals regional dissociation. Most notably liver was particularly rich in PBR-I yet contained no detectable MAO-AI. In addition we have succeeded in separating the four activities from human urine. MAO-AI and MAO-BI have greater retention on charcoal than PBR-I and CBR-I when eluted step-wise with aqueous methanol. MAO-AI can be separated from MAO-BI and in addition PBR-I can be separated from CBR-I by step aqueous methanol elution from Amberlite XAD-4. Hence we present two lines of evidence that, contrary to the original view, tribulin is composed of a number of distinct molecular components.
authors.affiliation: The Psychophysiology and Stress Research Group, School of Biosciences, University of Westminster, London, UK. hucklef@wmin.ac.uk
authors.forename: F
authors.initials: F
authors.lastname: Hucklebridge
authors.forename: A
authors.initials: A
authors.lastname: Doyle
authors.forename: F Y
authors.initials: FY
authors.lastname: Pang
authors.forename: M
authors.initials: M
authors.lastname: Adlard
authors.forename: P
authors.initials: P
authors.lastname: Evans
authors.forename: A
authors.initials: A
authors.lastname: Clow
mesh_terms: D000311:Adrenal Glands / Q000201:enzymology / Q000378:metabolism; D000818:Animals; D005260:Female; D058787:GABA-A Receptor Antagonists; D006801:Humans; D007420:Intestine, Large / Q000201:enzymology / Q000378:metabolism; D007510:Isatin; D008099:Liver / Q000201:enzymology / Q000378:metabolism; D008297:Male; D008996:Monoamine Oxidase Inhibitors / Q000302:isolation & purification / Q000378:metabolism* / Q000652:urine; D009928:Organ Specificity; D051381:Rats; D017208:Rats, Wistar; D011963:Receptors, GABA-A / Q000378:metabolism; D013154:Spleen / Q000201:enzymology / Q000378:metabolism
chemical_list: D058787:GABA-A Receptor Antagonists; D008996:Monoamine Oxidase Inhibitors; D011963:Receptors, GABA-A; D007510:Isatin; C039776:tribulin