[Control of buccal peroxidases by a bacterial NADH-hypothiocyanite oxidoreductase].
Oral peroxidases (myeloperoxidase, sialoperoxidase) catalyze thiocyanate peroxidation into hypothiocyanite which is bacteriostatic or bactericidal against numerous bacterial species. NADH-hypothiocyanite-oxidoreductase is thought to protect bacteria which can express it; up to now, this enzyme activity was never purified. The present study analyzes, on one hand, the susceptibility of periodontal bacteria against hypothiocyanite and, on the other hand, proposes a purification design for the NADH-hypothiocyanite-oxidoreductase from Streptococcus sanguis, a commensal micro-organism of dental surfaces. The data suggest the importance of the bacterial biofilm on dental surfaces for production of antiseptic oxidants and for the control of their toxicity.
Courtois
P
P
Laboratoire de recherche en Stomatologie, faculté de Médecine, Université libre de Bruxelles.
D000891:Anti-Infective Agents, Local / Q000494:pharmacology; D006801:Humans; D009061:Mouth Mucosa / Q000382:microbiology; D009247:NADH, NADPH Oxidoreductases / Q000302:isolation & purification / Q000494:pharmacology*; D010544:Peroxidases / Q000037:antagonists & inhibitors*; D012463:Saliva / Q000201:enzymology; D013298:Streptococcus sanguis / Q000201:enzymology
D000891:Anti-Infective Agents, Local; D010544:Peroxidases; D009247:NADH, NADPH Oxidoreductases; C103602:NADH-hypothiocyanite oxidoreductase
Contrôle des peroxydases buccales par une NADH-hypothiocyanite-oxydoréductase bactérienne.