Agar-gel-immunodiffusion and counterimmunoelectrophoresis for diagnosis of Newcastle disease.
Chickens were experimentally infected with Newcastle disease virus (NDV) and different organs were collected at point of death. Demonstration of NDV specific antigen in tissue samples were detected by haemagglutination (HA), agar-gel-immunodiffusion (AGID) and counterimmunoelectrophoresis (CIE) tests. Out of 51 samples tested 44, 27 and 37 were positive by HA, AGID and CIE respectively. The usefulness of AGID and CIE in Newcastle disease diagnosis is discussed.
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Centre for Animal Health Studies, Tamilnadu Veterinary and Animal Sciences University, Madhavaram Milk Colony, Madras, India.
Venugopalan
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D000362:Agar; D000818:Animals; D000956:Antigens, Viral / Q000032:analysis; D002645:Chickens; D003378:Counterimmunoelectrophoresis / Q000379:methods; D004198:Disease Susceptibility; D006386:Hemagglutination Tests; D005779:Immunodiffusion / Q000379:methods; D009521:Newcastle Disease / Q000175:diagnosis*; D009522:Newcastle disease virus / Q000302:isolation & purification* / Q000472:pathogenicity; D009928:Organ Specificity; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D014774:Virulence
D000956:Antigens, Viral; D000362:Agar