title: Human immunodeficiency virus (HIV) envelope binds to CXCR4 independently of CD4, and binding can be enhanced by interaction with soluble CD4 or by HIV envelope deglycosylation.
abstract: Chemokine receptor CXCR4 (also known as LESTR and fusin) has been shown to function as a coreceptor for T-cell-tropic strains of human immunodeficiency virus type 1 (HIV-1). We have developed a binding assay to show that HIV envelope (Env) can interact with CXCR4 independently of CD4 but that this binding is markedly enhanced by the previous interaction of Env with soluble CD4. We also show that nonglycosylated HIV-1(SF-2) gp120 or sodium metaperiodate-treated oligomeric gp160 from HIV-1(451) bound much more readily to CXCR4 than their counterparts with intact carbohydrate residues did.
authors.affiliation: Research Center for AIDS and HIV Infection, Manhattan VA Medical Center, and Department of Pathology, New York University, New York 10010, USA. bandrj01@mcrcr.med.nyu.edu
authors.forename: J C
authors.initials: JC
authors.lastname: Bandres
authors.forename: Q F
authors.initials: QF
authors.lastname: Wang
authors.forename: J
authors.initials: J
authors.lastname: O'Leary
authors.forename: F
authors.initials: F
authors.lastname: Baleaux
authors.forename: A
authors.initials: A
authors.lastname: Amara
authors.forename: J A
authors.initials: JA
authors.lastname: Hoxie
authors.forename: S
authors.initials: S
authors.lastname: Zolla-Pazner
authors.forename: M K
authors.initials: MK
authors.lastname: Gorny
mesh_terms: D000818:Animals; D015704:CD4 Antigens / Q000378:metabolism*; D002460:Cell Line; D006031:Glycosylation; D015699:HIV Envelope Protein gp120 / Q000378:metabolism*; D019302:HIV Envelope Protein gp160 / Q000378:metabolism*; D015497:HIV-1 / Q000378:metabolism*; D006801:Humans; D019169:Jurkat Cells; D051379:Mice; D008807:Mice, Inbred BALB C; D019718:Receptors, CXCR4 / Q000378:metabolism*; D012995:Solubility
chemical_list: D015704:CD4 Antigens; D015699:HIV Envelope Protein gp120; D019302:HIV Envelope Protein gp160; D019718:Receptors, CXCR4