title: The permease homologue Ssy1p controls the expression of amino acid and peptide transporter genes in Saccharomyces cerevisiae.
abstract: Amino acid transporters of the yeast plasma membrane (permeases) belong to a family of integral membrane proteins with pronounced structural similarity. We present evidence that a member of this family, encoded by the open reading frame (ORF) YDR160w (SSY1), is required for the expression of a set of transporter genes. Thus, deletion of the SSY1 gene causes loss of leucine-inducible transcription of the amino acid permease genes BAP2, TAT1 and BAP3 (ORF YDR046c) and the peptide transporter, PTR2. D-leucine can generate the signal without entering the cell. We propose that Ssy1p is situated in the plasma membrane and is involved in sensing leucine in the medium.
authors.affiliation: Department of Yeast Genetics, Carlsberg Laboratory, Gamle Carlsberg Vej 10, Copenhagen Valby, Denmark.
authors.forename: T
authors.initials: T
authors.lastname: Didion
authors.forename: B
authors.initials: B
authors.lastname: Regenberg
authors.forename: M U
authors.initials: MU
authors.lastname: Jørgensen
authors.forename: M C
authors.initials: MC
authors.lastname: Kielland-Brandt
authors.forename: H A
authors.initials: HA
authors.lastname: Andersen
mesh_terms: D026905:Amino Acid Transport Systems; D000596:Amino Acids / Q000378:metabolism; D002352:Carrier Proteins / Q000235:genetics* / Q000378:metabolism; D005656:Fungal Proteins / Q000378:metabolism; D017353:Gene Deletion; D015966:Gene Expression Regulation, Fungal; D005800:Genes, Fungal; D007930:Leucine / Q000378:metabolism; D008565:Membrane Proteins / Q000235:genetics* / Q000378:metabolism; D026901:Membrane Transport Proteins / Q000235:genetics* / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism; D029701:Saccharomyces cerevisiae Proteins; D015398:Signal Transduction; D014158:Transcription, Genetic; D015533:Transcriptional Activation
chemical_list: D026905:Amino Acid Transport Systems; D000596:Amino Acids; D002352:Carrier Proteins; D005656:Fungal Proteins; D008565:Membrane Proteins; D026901:Membrane Transport Proteins; C093495:PTR2 protein, S cerevisiae; D029701:Saccharomyces cerevisiae Proteins; D007930:Leucine