title: Artificial evolution by DNA shuffling.
abstract: Improvement of enzymes is one of the important objectives of biotechnology. In vitro evolution of enzymes using DNA shuffling involves the assembly of two or more DNA segments into a full-length gene by homologous, or site-specific, recombination. Before the assembly, the segments are often subjected to random mutagenesis by error-prone PCR, random nucleotide insertion or other methods. Many useful enzymes and peptides have been isolated following the artificial evolution.
authors.affiliation: Marine Biotechnology Institute, Iwate, Japan.
authors.forename: S
authors.initials: S
authors.lastname: Harayama
mesh_terms: D001435:Bacteriophages / Q000235:genetics; D005075:Biological Evolution; D005091:Exons; D015723:Gene Library; D016296:Mutagenesis; D016133:Polymerase Chain Reaction