title: Two rainbow trout (Oncorhynchus mykiss) albumin genes are differentially regulated.
abstract: Two distinct albumin cDNAs (rtALB1 and rtALB2) were isolated from the rainbow trout (Oncorhynchus mykiss) liver cDNA library. The rtALB1 cDNA (2761 bp) contains a 69 bp 5' untranslated region (UTR), a 1821 bp reading region, and a long 3' UTR of 872 bp. The rtALB2 cDNA (2250 bp) contains a 78 bp 5' UTR, a 1824 bp coding region, and a 348 bp 3' UTR. The two albumins are 81.5% and 77.5% identical in their nucleotides and protein sequences, respectively. Both rtALB1 and rtALB2 genes are expressed only in the liver. The albumin mRNA was first detected in 5-week-old embryos and was tissue-specific. The two albumin genes were differentially expressed, with the rtALB1 transcripts being 3 to 10 times more abundant than the rtALB2 transcripts. This differential expression was partially regulated at the transcriptional level. Promoter analysis showed that the rtALB1 gene had a typical albumin promoter structure. However, the rtALB2 promoter was abnormal in the TATA box region and was less effective in activating the reporter gene in the mammalian cell lines. These variations in rainbow trout albumin promoter sequences might account for their differences in transcriptional efficiency.
authors.affiliation: Research Institute, Hospital for Sick Children, and Department of Laboratory Medicine, University of Toronto, Ontario, Canada.
authors.forename: Z Q
authors.initials: ZQ
authors.lastname: Gong
authors.forename: C L
authors.initials: CL
authors.lastname: Hew
mesh_terms: D000418:Albumins / Q000235:genetics*; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000302:isolation & purification; D029941:Fish Proteins; D005786:Gene Expression Regulation; D018507:Gene Expression Regulation, Developmental; D008099:Liver / Q000378:metabolism; D008969:Molecular Sequence Data; D017686:Oncorhynchus mykiss / Q000235:genetics*; D011401:Promoter Regions, Genetic; D012333:RNA, Messenger / Q000378:metabolism
chemical_list: C110825:ALB1 protein, rainbow trout; C110826:ALB2 protein, rainbow trout; D000418:Albumins; D018076:DNA, Complementary; D029941:Fish Proteins; D012333:RNA, Messenger