[Change of hydroperoxy fatty acids formed by ultraviolet irradiation of bovine retinas--determination of chemiluminescence assay].
Hydroperoxy fatty acids possibly formed by light-induced retinal damage have been measured with conventional methods which could not detect directly individual hydroperoxy fatty acids. We tried to measure directly the retinal hydroperoxy fatty acids using chemiluminescence/high-performance liquid chromatography. A total of 30 bovine retinas were used for the following experiment. First, the effects of autoxidation on the content of fatty acids in the retinas were confirmed to be significantly negligible, and then the retinas were divided into 3 groups. The first and second groups were exposed to ultraviolet (UV) irradiation for 12 and 24 hrs respectively, and the third was not irradiated as a control to see if increases in hydroperoxy fatty acids and decreases in fatty acids can be caused by the irradiation. The amount of unsaturated fatty acids per one retina was clearly decreased after 24 hrs of irradiation. The amount of hydroperoxy fatty acid of 18 carbon atoms increased in proportion to the irradiation time, but that of 20-22 carbon atoms was not different between 12 hrs and 24 hrs of irradiation. The detected amount of hydroperoxy fatty acids did not correspond to that of normal acids, which decreased. This suggests that hydroperoxy fatty acids could be split after their formation during the UV irradiation.
Kobayashi
Y
Y
Department of Ophthalmology, Kawasaki Medical School, Okayama-ken, Japan.
D000818:Animals; D002417:Cattle; D005227:Fatty Acids / Q000032:analysis*; D066298:In Vitro Techniques; D015227:Lipid Peroxidation; D008163:Luminescent Measurements; D012160:Retina / Q000737:chemistry / Q000528:radiation effects*; D014466:Ultraviolet Rays
D005227:Fatty Acids