title: Isolation of recombinant secretory proteins by limited induction and quantitative harvest.
authors.affiliation: Laboratory of Host Defenses, NIAID/NIH, Bethesda, MD 20892, USA.
authors.forename: H F
authors.initials: HF
authors.lastname: Rosenberg
mesh_terms: D001426:Bacterial Proteins / Q000235:genetics / Q000302:isolation & purification; D047208:Eosinophil-Derived Neurotoxin; D004926:Escherichia coli / Q000235:genetics*; D005786:Gene Expression Regulation / Q000235:genetics*; D007544:Isopropyl Thiogalactoside / Q000494:pharmacology; D009498:Neurotoxins / Q000235:genetics; D009842:Oligopeptides; D010455:Peptides / Q000235:genetics / Q000276:immunology; D019897:Periplasm / Q000737:chemistry; D010957:Plasmids / Q000235:genetics; D021382:Protein Sorting Signals / Q000235:genetics; D011994:Recombinant Proteins / Q000302:isolation & purification / Q000378:metabolism*; D012260:Ribonucleases / Q000378:metabolism
chemical_list: D001426:Bacterial Proteins; D009498:Neurotoxins; D009842:Oligopeptides; D010455:Peptides; D021382:Protein Sorting Signals; D011994:Recombinant Proteins; D007544:Isopropyl Thiogalactoside; C085706:FLAG peptide; D047208:Eosinophil-Derived Neurotoxin; D012260:Ribonucleases