Potent suppressive activity of nonpolyphenolic fraction of green tea (Camellia sinensis) against genotoxin-induced umu C gene expression in Salmonella typhimurium (TA 1535/pSK 1002), tumor promotor-dependent ornithine decarboxylase induction of BALB/c 3T3 fibroblast cells, and chemically induced mouse skin tumorigenesis.
Many experimental studies for anticarcinogenic activity of green tea (Camellia sinensis) and tea-derived polyphenols have been carried out. However, the anticarcinogenic activity of the nonpolyphenolic fraction of green tea has been poorly elucidated. To study this problem, the effect of the nonpolyphenolic fraction of green tea leaves was analyzed using in vitro and in vivo experiments associated with tumor initiation and promotion as follows: 1) The nonpolyphenolic fraction caused a strong suppressive effect on umu C gene expression in Salmonella typhimurium (TA 1535/pSK 1002) induced by genotoxic substances such as 2-amino-6-methyldipirido[1,2-a:3',2'-d]imidazole (Glu-P-1) and 2-aminoanthracene (2-AA) in the presence of a hepatic metabolizing enzyme mixture. 2) The same fraction showed a dose-dependent inhibition of ornithine decarboxylase (ODC) in BALB/c 3T3 fibroblasts induced by a tumor promotor, 12-O-tetradecanoylphorbol-13-acetate (TPA). 3) The same fraction also exhibited a significant suppression against mouse skin tumorigenesis induced by 7,12-dimethylbenz[a]anthracene (DMBA) (initiator) and TPA (promotor) through inhibition at both stages of tumor initiation and promotion. These results suggest that the nonpolyphenolic fraction of green tea has a potent suppressing activity against carcinogenesis associated with tumor initiation and promotion.
Okai
Y
Y
Department of Human Life Science, Osaka Kun-Ei Women's College, Japan.
Higashi-Okai
K
K
D016475:3T3 Cells; D015127:9,10-Dimethyl-1,2-benzanthracene; D000818:Animals; D016588:Anticarcinogenic Agents / Q000494:pharmacology*; D001426:Bacterial Proteins / Q000235:genetics*; D004259:DNA-Directed DNA Polymerase; D004790:Enzyme Induction / Q000187:drug effects; D029968:Escherichia coli Proteins; D005260:Female; D015870:Gene Expression / Q000187:drug effects; D051379:Mice; D008807:Mice, Inbred BALB C; D009955:Ornithine Decarboxylase / Q000096:biosynthesis*; D012486:Salmonella typhimurium / Q000235:genetics; D012878:Skin Neoplasms / Q000139:chemically induced / Q000517:prevention & control*; D013662:Tea; D013755:Tetradecanoylphorbol Acetate / Q000633:toxicity
D016588:Anticarcinogenic Agents; D001426:Bacterial Proteins; D029968:Escherichia coli Proteins; D013662:Tea; D015127:9,10-Dimethyl-1,2-benzanthracene; C078202:UmuC protein, E coli; D004259:DNA-Directed DNA Polymerase; D009955:Ornithine Decarboxylase; D013755:Tetradecanoylphorbol Acetate