Response of diencephalon but not the gonad to female-promoting temperature with elevated estradiol levels in the sea turtle Lepidochelys olivacea.
Although temperature sex determination is well known in several reptile species, the physiological mechanism underlying this process remains to be elucidated. In the current work, we analyzed the levels of testosterone (T) and estradiol (E2) in the gonads; two brain regions--telencephalon (Te) and diencephalon/mesencephalon (Di)--and the serum of developing embryos of the olive ridley Lepidochelys olivacea incubated at male- or female-promoting temperatures. Conversion of pregnenolone (P5) to T and T to E2 were studied in the gonads and brain. The analyses were performed during three periods: the thermosensitive period (TSP), histologically undifferentiated gonads (UDG), and differentiated gonads (DG). In the gonads, serum, and brain, T concentrations were higher at the female-promoting temperature during the three periods, whereas in the gonads and serum, E2 levels were similar at the female and male-promoting temperature. In Di, the concentration of E2 was significantly higher at the female-promoting temperature. Biotransformation of P5 to T in gonadal tissues were slightly higher at the female-promoting temperature in TSP and increased during UDG and DG. Conversion of T to E2, however, was similar at the two temperatures during the three periods. In the brain, the Di showed a higher efficiency for transforming T to E2 at the female-promoting temperature. Our present results do not allow us to decide whether the diencephalon is the cause or the effect, but they conclusively demonstrate that, in L. olivacea, this region of the brain senses temperature during sex determination.
Salame-Mendez
A
A
Department of Biology of Reproduction, Universidad Autónoma Metropolitana-Iztapalapa, México, D.F. México.
Herrera-Munoz
J
J
Moreno-Mendoza
N
N
Merchant-Larios
H
H
D000818:Animals; D004027:Diencephalon / Q000378:metabolism*; D004958:Estradiol / Q000097:blood / Q000378:metabolism*; D005260:Female; D006066:Gonads / Q000196:embryology / Q000378:metabolism*; D008297:Male; D008636:Mesencephalon / Q000378:metabolism; D011284:Pregnenolone / Q000378:metabolism; D019849:Sex Determination Processes; D013687:Telencephalon / Q000378:metabolism; D013696:Temperature; D013739:Testosterone / Q000097:blood / Q000378:metabolism; D014426:Turtles
D013739:Testosterone; D004958:Estradiol; D011284:Pregnenolone