Comparison of cardiac Troponin T and cardiac Troponin I concentrations in peripheral blood during orciprenaline induced tachycardia in rats.
In this study we addressed the question of whether the measurement of the cardiac structure protein cardiac Toponin I (cTnI) in serum is also able to detect marked myocardial cell damage in rats like cardiac Troponin T (cTnT). To answer this question 5 male rats and 5 female rats were injected with orciprenaline to induce myocardial cell damage. Further 5 animals of each sex received physiological saline as control. We could demonstrate that troponin I was rised significantly 6 hours after injection in serum as well as cTnT. 24 hours after injection cTnI was elevated on a lower significance level compared to cTnT. All values returned to normal 96 hours after the injection. We conclude that cTnI was also able to detect a marked myocardial cell damage in rats but we could show that cTnI was elevated on a lower significance level compared to cTnT. Furthermore we found that a cTnI increase to at least 4.1 ng/ml is necessary to detect marked myocardial cell injury in this rat tachycardia model.
Bertsch
T
T
Institute for Clinical Chemistry at the Mannheim Municipal Hospital, Faculty of Clinical Medicine of the University of Heidelberg, Germany.
Bleuel
H
H
Aufenanger
J
J
Rebel
W
W
D000818:Animals; D015415:Biomarkers / Q000097:blood; D005260:Female; D008297:Male; D009921:Metaproterenol / Q000633:toxicity*; D009206:Myocardium / Q000473:pathology; D051381:Rats; D011933:Reagent Kits, Diagnostic; D013566:Sympathomimetics / Q000633:toxicity*; D013610:Tachycardia / Q000097:blood / Q000139:chemically induced* / Q000473:pathology; D014336:Troponin / Q000097:blood*; D019210:Troponin I / Q000097:blood*; D020107:Troponin T
D015415:Biomarkers; D011933:Reagent Kits, Diagnostic; D013566:Sympathomimetics; D014336:Troponin; D019210:Troponin I; D020107:Troponin T; D009921:Metaproterenol