Resolution of the diastereomers of a large synthetic peptide by capillary electrophoresis using nonionic surfactants.
A capillary electrophoretic method was developed for the determination of the diastereomeric composition of the bradykinin B2 antagonist, SB-238592-DB. The nonionic surfactant Brij 35 was demonstrated to be suitable for the baseline resolution of the three diastereoisomers of SB-238592-DB in the presence of a low pH phosphate buffer and small volume percentages of acetonitrile.
Gilges
M
M
Department of Analytical Sciences, SmithKline Beecham Pharmaceuticals, Tonbridge, Kent, UK. martin_gilges-1@sbphrd.com
Hadley
M
M
D000595:Amino Acid Sequence; D065168:Bradykinin Receptor Antagonists; D002021:Buffers; D019075:Electrophoresis, Capillary; D006863:Hydrogen-Ion Concentration; D007477:Ions; D016014:Linear Models; D008969:Molecular Sequence Data; D009842:Oligopeptides / Q000302:isolation & purification*; D010710:Phosphates; D000077423:Polidocanol; D011092:Polyethylene Glycols; D013237:Stereoisomerism; D013501:Surface-Active Agents
D065168:Bradykinin Receptor Antagonists; D002021:Buffers; D007477:Ions; D009842:Oligopeptides; D010710:Phosphates; D013501:Surface-Active Agents; D000077423:Polidocanol; D011092:Polyethylene Glycols