title: Broad immunocytochemical localization of the formylpeptide receptor in human organs, tissues, and cells.
abstract: The formylpeptide receptor (FPR), previously found only on polymorphonuclear leukocytes and monocytes/macrophages, responds to both synthetic N-formyl oligopeptides and those produced by bacteria. The cDNA for human FPR has been cloned and a rabbit polyclonal antiserum directed against a synthetic 11-amino-acid peptide corresponding to the deduced carboxy-terminus has been produced. We have now extensively characterized and used the antibody to detect FPR on normal human tissues and cell types. The receptor antigen is present on some epithelial cells, especially those with a secretory function, and on some endocrine cells, e.g., follicular cells of the thyroid and cortical cells of the adrenal. Liver hepatocytes and Kupffer cells are positive. Smooth muscle and endothelial cells are also generally positive. In the brain and spinal cord, the neurons of the motor, sensory, and cerebellar systems, and those of the parasympathetic and sympathetic systems stain positively. These data suggest that the putative endogenous agonist for FPR or an antigenically similar receptor reacts with cellular targets in the neuromuscular, vascular, endocrine, and immune systems.
authors.affiliation: Department of Pathology, University of Connecticut Health Center Farmington, 263 Farmington Avenue, Farmington, CT 06030-3105, USA.
authors.forename: E L
authors.initials: EL
authors.lastname: Becker
authors.forename: F A
authors.initials: FA
authors.lastname: Forouhar
authors.forename: M L
authors.initials: ML
authors.lastname: Grunnet
authors.forename: F
authors.initials: F
authors.lastname: Boulay
authors.forename: M
authors.initials: M
authors.lastname: Tardif
authors.forename: B J
authors.initials: BJ
authors.lastname: Bormann
authors.forename: D
authors.initials: D
authors.lastname: Sodja
authors.forename: R D
authors.initials: RD
authors.lastname: Ye
authors.forename: J R
authors.initials: JR
authors.lastname: Woska
authors.forename: P M
authors.initials: PM
authors.lastname: Murphy
mesh_terms: D000941:Antigens / Q000096:biosynthesis; D001665:Binding Sites; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D008297:Male; D009240:N-Formylmethionine Leucyl-Phenylalanine / Q000378:metabolism*; D009447:Neuroblastoma / Q000737:chemistry / Q000378:metabolism; D009928:Organ Specificity; D011485:Protein Binding; D044042:Receptors, Formyl Peptide; D011971:Receptors, Immunologic / Q000096:biosynthesis / Q000276:immunology / Q000378:metabolism*; D018000:Receptors, Peptide / Q000096:biosynthesis / Q000276:immunology / Q000378:metabolism*; D014407:Tumor Cells, Cultured
chemical_list: D000941:Antigens; D044042:Receptors, Formyl Peptide; D011971:Receptors, Immunologic; D018000:Receptors, Peptide; D009240:N-Formylmethionine Leucyl-Phenylalanine