Comparable sensitivity and specificity of nested PCR and single-stage PCR using a thermally activated DNA polymerase.
Zimmermann
K
K
Department of Molecular Analysis of Infectious Processes, IMMUNO AG, Vienna, Austria.
Mannhalter
J W
JW
D017931:DNA Primers / Q000737:chemistry; D004259:DNA-Directed DNA Polymerase / Q000378:metabolism*; D004789:Enzyme Activation; D015686:Gene Products, env / Q000235:genetics; D015683:Gene Products, gag / Q000235:genetics; D015684:Gene Products, pol / Q000235:genetics; D015497:HIV-1 / Q000737:chemistry; D016133:Polymerase Chain Reaction / Q000379:methods*; D012680:Sensitivity and Specificity; D013696:Temperature
D017931:DNA Primers; D015686:Gene Products, env; D015683:Gene Products, gag; D015684:Gene Products, pol; D004259:DNA-Directed DNA Polymerase