Campylobacter jejuni 72Dz/92 cjaC gene coding 28 kDa immunopositive protein, a homologue of the solute-binding components of the ABC transport system.
Screening of the Campylobacter jejuni 72Dz/92 cosmid gene bank enabled identification of the cjaC (Camp. jejuni antigen C) gene encoding a highly immunogenic protein of apparent molecular mass 28 kDa. Gene bank searches indicated significant overall homology of the cjaC gene product, mainly to the Gram-negative periplasmic solute-binding proteins of the ABC transport system which recognize polar amino acids and opines. CjaC protein contains the motif LVAC at the end of the putative 19 amino acid signal sequence, which suggests that it might be a lipoprotein. In the 5' flanking region of the cjaC gene, two potential promoter regions were observed. The cjaC gene is conserved among some isolates of three serotypes commonly isolated from humans (HL serotypes 1,4,71).
Pawelec
D
D
Institute of Microbiology, Warsaw University, Poland. pawelec@plearn.edu.pl
Jakubowska-Mróz
J
J
Jagusztyn-Krynicka
E K
EK
D018528:ATP-Binding Cassette Transporters / Q000737:chemistry* / Q000235:genetics*; D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000737:chemistry* / Q000235:genetics* / Q000276:immunology; D001483:Base Sequence; D001692:Biological Transport; D015153:Blotting, Western; D016123:Campylobacter jejuni / Q000737:chemistry / Q000235:genetics*; D017124:Conserved Sequence; D015723:Gene Library; D006801:Humans; D008074:Lipoproteins / Q000737:chemistry* / Q000235:genetics*; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D015183:Restriction Mapping; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid
D018528:ATP-Binding Cassette Transporters; D001426:Bacterial Proteins; C111021:CjaC protein, Campylobacter jejuni; D008074:Lipoproteins