[Restriction endonuclease Sse9I (correction of Sse91) from Sporosarcina strain SP.9D recognizes the 5'-AATT-3' DNA sequence].
Sse91, a type II restriction endonuclease, has been isolated from Sporosarcina species 9D. The recognition sequence and cleavage point of restriction endonuclease Sse91 have been determined as 5'-decrease AATT-3'. The new enzyme is an isoschizomer of Tsp5091, but its optimal incubation temperature is 55 degrees C and it is inactivated at 65 degrees C for 20 min.
Gonchar
D A
DA
Dedkov
V S
VS
Verkhozina
V A
VA
Kusner
Iu S
IuS
Shevchenko
A V
AV
Degtiarev
S Kh
SKh
D004247:DNA / Q000378:metabolism*; D016936:Gram-Positive Endospore-Forming Bacteria / Q000201:enzymology*; D006358:Hot Temperature; D006868:Hydrolysis; D015265:Site-Specific DNA-Methyltransferase (Adenine-Specific) / Q000037:antagonists & inhibitors / Q000378:metabolism*; D013379:Substrate Specificity
D004247:DNA; C100875:DNA methyltransferase SSe9I; D015265:Site-Specific DNA-Methyltransferase (Adenine-Specific)
Endonukleaza restriktsii Sse91 iz shtamma Sporosarcina SP.9D uznaet posledovatel'nost' DNK 5'-AATT-3'.