title: Escherichia coli transformant expressing the glucose dehydrogenase gene from Bacillus megaterium as a cofactor regenerator in a chiral alcohol production system.
abstract: Escherichia coli JM109 (pGDA2) overexpressing the glucose dehydrogenase (GDH) gene from Bacillus megaterium IWG3 was examined for use as a cofactor regenerator. In the asymmetric reduction of ethyl 4-chloro-3-oxobutanoate by E. coli JM109 (pKAR) which is an aldehyde reductase-overproducing transformant, E. coli JM109 (pGDA2) can act as an NADPH regenerator with NADP+ and glucose, similarly to commercially available GDH.
authors.affiliation: Division of Applied Life Sciences, Graduate School of Agriculture, Kyoto University, Japan.
authors.forename: M
authors.initials: M
authors.lastname: Kataoka
authors.forename: L P
authors.initials: LP
authors.lastname: Sri Rohani
authors.forename: M
authors.initials: M
authors.lastname: Wada
authors.forename: K
authors.initials: K
authors.lastname: Kita
authors.forename: H
authors.initials: H
authors.lastname: Yanase
authors.forename: I
authors.initials: I
authors.lastname: Urabe
authors.forename: S
authors.initials: S
authors.lastname: Shimizu
mesh_terms: D000438:Alcohols / Q000378:metabolism*; D001410:Bacillus megaterium / Q000201:enzymology* / Q000235:genetics; D004926:Escherichia coli / Q000378:metabolism*; D015870:Gene Expression; D005948:Glucose Dehydrogenases / Q000096:biosynthesis / Q000235:genetics / Q000378:metabolism*; D009249:NADP / Q000096:biosynthesis*
chemical_list: D000438:Alcohols; D009249:NADP; D005948:Glucose Dehydrogenases