title: Immunochemical studies of the lipopolysaccharide O-specific polysaccharide of Hafnia alvei PCM 1199 related to H. alvei PCM 1205.
abstract: On the basis of chemical analyses and NMR spectroscopic studies, it was found that the O-specific polysaccharide (O-PS) isolated from the Hafnia alvei PCM 1199 lipopolysaccharide (LPS) is a glycerol teichoic-acid-like polymer having a repeating unit of the following structure: [structure in text] where Qui4NAc is 4-acetamido-4,6-dideoxyglucose and O-acetylation at both positions is non-stoichiometric. The glycosidic linkage of the lateral beta-D-GlcpNAc residue is acid-labile and cleaved from the O-PS during mild acid hydrolysis or dephosphorylation with 48% hydrofluoric acid. Comparative analysis revealed that the structure of the H. alvei PCM 1199 O-PS is similar to that of H. alvei PCM 1205, which differs in the presence of an additional lateral alpha-D-Glcp residue and the position of one of the O-acetyl groups only. Accordingly, serological tests revealed a high degree of serological similarity between LPSs and O-PSs of the two H. alvei strains.
authors.affiliation: L. Hirszfeld Institute of Immunology and Experimental Therapy, Polish Academy of Sciences, Wroclaw. katzenel@immuno.iitd.pan.wroc.pl
authors.forename: E
authors.initials: E
authors.lastname: Katzenellenbogen
authors.forename: N A
authors.initials: NA
authors.lastname: Kocharova
authors.forename: G V
authors.initials: GV
authors.lastname: Zatonsky
authors.forename: M
authors.initials: M
authors.lastname: Mieszala
authors.forename: A
authors.initials: A
authors.lastname: Gamian
authors.forename: M
authors.initials: M
authors.lastname: Bogulska
authors.forename: A S
authors.initials: AS
authors.lastname: Shashkov
authors.forename: E
authors.initials: E
authors.lastname: Romanowska
authors.forename: Y A
authors.initials: YA
authors.lastname: Knirel
mesh_terms: D002236:Carbohydrate Conformation; D002240:Carbohydrate Sequence; D002241:Carbohydrates / Q000032:analysis; D004755:Enterobacteriaceae / Q000737:chemistry* / Q000276:immunology; D008745:Methylation; D008969:Molecular Sequence Data; D019081:O Antigens / Q000737:chemistry* / Q000302:isolation & purification; D013045:Species Specificity; D013682:Teichoic Acids / Q000737:chemistry
chemical_list: D002241:Carbohydrates; D019081:O Antigens; D013682:Teichoic Acids