Identification of cytochrome P450 1A1 in human brain.
We have isolated cDNA fragments that were originated from P450 1A1 in human brain by polymerase chain reaction (PCR) analysis. Their size matched up to what we expected based on the reported P450 1A1 mRNA sequence of human breast carcinoma cell line MCF-7. To confirm proper PCR products, they were subcloned and their nucleotide sequences were determined. This result showed that PCR products were actually derived from P450 1A1 mRNA in human brain. We demonstrated for the first time that human brain contains a P450 1A1-dependent metabolism system which seems to be important for understanding the metabolism of endogenous and exogenous compounds.
Yun
C H
CH
Department of Biochemistry, Pai-Chai University, Taejon, Korea. chyun@woonam.paichai.ac.kr
Park
H J
HJ
Kim
S J
SJ
Kim
H K
HK
D001483:Base Sequence; D015139:Blotting, Southern; D001921:Brain / Q000201:enzymology*; D001943:Breast Neoplasms / Q000201:enzymology; D019363:Cytochrome P-450 CYP1A1 / Q000235:genetics* / Q000378:metabolism; D004247:DNA / Q000032:analysis; D018076:DNA, Complementary / Q000737:chemistry; D015870:Gene Expression; D006801:Humans; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000032:analysis / Q000737:chemistry; D016415:Sequence Alignment; D014407:Tumor Cells, Cultured
D018076:DNA, Complementary; D012333:RNA, Messenger; D004247:DNA; D019363:Cytochrome P-450 CYP1A1