title: Human platelets damage Aspergillus fumigatus hyphae and may supplement killing by neutrophils.
abstract: Neutropenia is considered a significant risk factor for invasive aspergillosis but is almost always associated with concurrent thrombocytopenia. Studies determined that platelets, like neutrophils, attached to cell walls of the invasive hyphal form of Aspergillus fumigatus. Organisms were damaged as shown by loss of cell wall integrity in scanning laser confocal microscopy and release of defined hyphal surface glycoproteins. Rapid expression appearance of surface antigen CD63 and release of markers of platelet degranulation confirmed activation during attachment to hyphae. Optimal platelet activation required opsonization of hyphae with fresh or heat-inactivated whole plasma. These effects of opsonization with whole plasma could not be duplicated by pooled human serum, immunoglobulin G, or fibrinogen, whether used separately or combined. Thus, platelets in the presence of whole plasma have the potential to play an important role in normal host defenses against invasive aspergillosis.
authors.affiliation: Department of Medicine, Boston Medical Center, and Boston University School of Medicine, Massachusetts 02118, USA.
authors.forename: L
authors.initials: L
authors.lastname: Christin
authors.forename: D R
authors.initials: DR
authors.lastname: Wysong
authors.forename: T
authors.initials: T
authors.lastname: Meshulam
authors.forename: R
authors.initials: R
authors.lastname: Hastey
authors.forename: E R
authors.initials: ER
authors.lastname: Simons
authors.forename: R D
authors.initials: RD
authors.lastname: Diamond
mesh_terms: D001232:Aspergillus fumigatus / Q000276:immunology*; D001792:Blood Platelets / Q000502:physiology*; D015550:Cell Degranulation; D006801:Humans; D009504:Neutrophils / Q000276:immunology*; D015539:Platelet Activation; D010973:Platelet Adhesiveness; D013778:Tetrazolium Salts / Q000378:metabolism
chemical_list: D013778:Tetrazolium Salts; C059087:2,3-bis(2-methoxy-4-nitro-5-sulfophenyl)-5-((phenylamino)carbonyl)-2H-tetrazolium hydroxide