title: Cell membrane lipid molecular dynamics in a solenoid versus a magnetically shielded room.
abstract: The generalized polarization function of the fluorescent probe 2-dimethylamino-6-lauroylnaphthalene has been used to evaluate the lipid dynamics in Friend erythroleukemia cell membrane. The values of this function varied during the culture growth cycle, showing decreased lipid dynamics 24-48 h from the cell seeding. When the cycle occurred in a solenoid producing a magnetic field of 70 microT at 50 Hz in addition to the 45 microT DC of the earth (short-term 4-day exposure), the membrane lipid dynamics during this same time-period decreased by about 10% (P < .04). After long-term (184 days) or extremely long-term (395 days) exposure of the cells to the magnetic field, little additional variation in the membrane lipid dynamics was observed, suggesting an adaptation phenomenon. A variation of membrane lipid dynamics was also observed due to in vitro cell differentiation (P < .02). Nevertheless, the exposure of both undifferentiating and differentiating cells to a highly attenuated magnetic field in a magnetically shielded room (20 nT DC plus 2.5 pT AC) did not induce any modification of membrane lipid dynamics.
authors.affiliation: Department of Biology of the University of Rome Tor Vergata, Italy.
authors.forename: P
authors.initials: P
authors.lastname: Volpe
authors.forename: T
authors.initials: T
authors.lastname: Parasassi
authors.forename: C
authors.initials: C
authors.lastname: Esposito
authors.forename: G
authors.initials: G
authors.lastname: Ravagnan
authors.forename: A M
authors.initials: AM
authors.lastname: Giusti
authors.forename: A
authors.initials: A
authors.lastname: Pasquarelli
authors.forename: T
authors.initials: T
authors.lastname: Eremenko
mesh_terms: D000818:Animals; D002453:Cell Cycle / Q000528:radiation effects; D002454:Cell Differentiation / Q000528:radiation effects; D002455:Cell Division / Q000528:radiation effects; D002462:Cell Membrane / Q000378:metabolism / Q000528:radiation effects; D004553:Electric Conductivity; D004574:Electromagnetic Fields / Q000009:adverse effects; D004780:Environment, Controlled; D004915:Leukemia, Erythroblastic, Acute / Q000378:metabolism / Q000473:pathology; D008563:Membrane Lipids / Q000378:metabolism* / Q000528:radiation effects; D051379:Mice; D013997:Time Factors; D014407:Tumor Cells, Cultured
chemical_list: D008563:Membrane Lipids