Parathyroid hormone (1-34) receptor-binding and second-messenger response in rat incisor odontoblasts.
Even though indirect evidence indicates that PTH exerts an anabolic effect on dentinogenesis, the existence of PTH receptors and any second-messenger response in odontoblasts have not been demonstrated. The aim of this study was to investigate whether rat incisor odontoblasts express PTH receptors, and to identify which second messenger pathway the hormone may activate. Odontoblasts were dissected from rat incisors. Amino-terminal (1-34) fragment rat PTH [rPTH(1-34)] conjugated to fluorescein isothiocyanate visualized receptor sites on the cell surface. Upon incubation of odontoblasts with rPTH(1-34), cAMP formation was increased. However, no fluctuations in intracellular calcium activity were observed upon rPTH(1-34) stimulation when using Fura-2 as a Ca2+ probe. In long-time incubations, stimulation with PTH(1-34) upregulated APase activity. The results demonstrate that rPTH(1-34) evokes an anabolic response in dentinogenically active odontoblasts, and that this may be mediated through the protein kinase A/cAMP pathway, whereas no indications for Ca2+ as a second messenger were evident.
Lundgren
T
T
Department of Oral Biochemistry, Faculty of Odontology, Göteborg University, Sweden.
Stenport
V
V
Wetter
A
A
Linde
A
A
D000469:Alkaline Phosphatase / Q000378:metabolism; D000818:Animals; D001667:Binding, Competitive; D002118:Calcium / Q000378:metabolism; D000242:Cyclic AMP / Q000378:metabolism*; D007180:Incisor / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D009804:Odontoblasts / Q000187:drug effects / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley; D018016:Receptors, Parathyroid Hormone / Q000378:metabolism*; D015290:Second Messenger Systems / Q000187:drug effects*; D019379:Teriparatide / Q000494:pharmacology*; D015854:Up-Regulation
D018016:Receptors, Parathyroid Hormone; D019379:Teriparatide; D000242:Cyclic AMP; D000469:Alkaline Phosphatase; D002118:Calcium