Polymorphisms of the genes encoding cruzipain, the major cysteine proteinase of Trypanosoma cruzi, in the region encoding the C-terminal domain.
Forty-eight cDNA clones obtained from different developmental stages of Trypanosoma cruzi and all encoding the C-terminal domain of the major cysteine proteinase (cruzipain) have been sequenced. A number of polymorphisms were detected, seven of them resulting in amino acid replacements. The predicted pI values of the corresponding gene products varied between 7.05 and 8.12. These changes in amino acid sequence, together with previously reported variations in carbohydrate composition at the only N-glycosylation site in the C-terminal domain, may account for most of the heterogeneities found in the mature enzyme.
Martínez
J
J
Instituto de Investigaciones Bioquímicas Luis F. Leloir, CONICET, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Buenos Aires, Argentina.
Henriksson
J
J
Ridåker
M
M
Pettersson
U
U
Cazzulo
J J
JJ
D000818:Animals; D000953:Antigens, Protozoan / Q000737:chemistry / Q000235:genetics; D003001:Cloning, Molecular; D003546:Cysteine Endopeptidases / Q000737:chemistry / Q000235:genetics*; D018076:DNA, Complementary; D016054:DNA, Protozoan / Q000032:analysis; D008969:Molecular Sequence Data; D011110:Polymorphism, Genetic; D017434:Protein Structure, Tertiary; D015800:Protozoan Proteins; D017386:Sequence Homology, Amino Acid; D014349:Trypanosoma cruzi / Q000201:enzymology / Q000235:genetics*
D000953:Antigens, Protozoan; D018076:DNA, Complementary; D016054:DNA, Protozoan; D015800:Protozoan Proteins; D003546:Cysteine Endopeptidases; C063214:cruzipain