Genetic characterization of the malaria parasite Plasmodium falciparum in the transmission from the host to the vector.
The present study followed a Plasmodium falciparum population through its life-cycle at the level of individual hosts and vectors. The aim was to determine to what extent genotypes of the parasite that were found in the host were transmitted to the vector. Mosquitoes were collected that had fed on people sleeping under mosquito nets with holes. Genes determining 2 highly polymorphic merozoite surface proteins, MSP-1 and MSP-2, were used to characterize the parasite by PCR at different stages of the life-cycle. The parasite genotypes found in the host were compared to the genotypes of the oocyst stages after transmission to the mosquitoes. The results show that there was no significant correlation in the rate of oocyst-positive mosquitoes and the presence of parasites in the blood samples. For MSP-1, most of the parasites characterized in the human blood by PCR fragment size were also found afterwards in the oocyst-stage (22 of 29; MSP-2: 17 of 36). This study indicates that there is no selective transmission of distinct genotypes to the vector. In addition, the frequencies of the allelic families of both genes are similar in the blood samples and in the oocysts for the whole population.
Huber
W
W
Swiss Tropical Institute, Department of Public Health and Epidemiology, Basel, Switzerland. infhuw@usz.unizh.ch
Haji
H
H
Charlwood
J D
JD
Certa
U
U
Walliker
D
D
Tanner
M
M
D000293:Adolescent; D000328:Adult; D000483:Alleles; D000818:Animals; D000852:Anopheles / Q000469:parasitology*; D000953:Antigens, Protozoan; D002648:Child; D002675:Child, Preschool; D005260:Female; D005819:Genetic Markers; D005838:Genotype; D006801:Humans; D007303:Insect Vectors / Q000469:parasitology*; D016778:Malaria, Falciparum / Q000469:parasitology / Q000635:transmission; D020066:Merozoite Surface Protein 1; D008875:Middle Aged; D010963:Plasmodium falciparum / Q000235:genetics* / Q000502:physiology; D016133:Polymerase Chain Reaction; D011498:Protein Precursors / Q000097:blood / Q000235:genetics*; D015800:Protozoan Proteins / Q000097:blood / Q000235:genetics*
D000953:Antigens, Protozoan; D005819:Genetic Markers; D020066:Merozoite Surface Protein 1; D011498:Protein Precursors; D015800:Protozoan Proteins; C065974:merozoite surface protein 2, Plasmodium