Interferon-beta is required for interferon-alpha production in mouse fibroblasts.
The type I interferons--interferon-alpha (IFN-alpha) and interferon-beta (IFN-beta)--are critical for protection against viruses during the acute stage of viral infection [1,2]. Furthermore, type I interferons have been implicated as important mediators in the regulation of lymphocyte development [3], immune responses [4,5] and the maintenance of immunological memory of cytotoxic T cells [6,7]. The different IFN-alpha subtypes are encoded by 12 genes in the mouse [8] whereas IFN-beta is encoded by only one gene [9]. IFN-alpha and IFN-beta have a high degree of sequence homology and are thought to interact with the same surface receptor on target cells [10,11]. As an approach to analysing the different biological functions of IFN-alpha and IFN-beta, we have generated a mouse strain with an inactivated IFN-beta gene. We report here that embryonic fibroblasts from such mice produce neither IFN-beta nor IFN-alpha upon Sendal virus infection, whereas the production of IFN-alpha by leukocytes from the same strain of mice is intact. IFN-alpha production in embryonic fibroblasts from IFN-beta-/- mice could be rescued by 'priming' the cells using exogenous IFN-beta. These results imply a unique role for IFN-beta in the induction of type I interferons in peripheral tissues.
Erlandsson
L
L
Immunology Group, CMB, Lund University, Sweden. Leandersson@immuno.lu.se
Blumenthal
R
R
Eloranta
M L
ML
Engel
H
H
Alm
G
G
Weiss
S
S
Leanderson
T
T
D000818:Animals; D004622:Embryo, Mammalian / Q000166:cytology / Q000378:metabolism* / Q000821:virology; D005347:Fibroblasts / Q000378:metabolism / Q000821:virology; D016898:Interferon-alpha / Q000096:biosynthesis*; D016899:Interferon-beta / Q000235:genetics / Q000502:physiology*; D051379:Mice; D016296:Mutagenesis; D018108:Respirovirus / Q000502:physiology
D016898:Interferon-alpha; D016899:Interferon-beta