Postnatal development of Schwann cells at neuromuscular junctions, with special reference to synapse elimination.
The neuromuscular junctions (NMJs) of postnatal rat soleus muscles were examined by immunohistochemical staining for S100, a marker of Schwann cells (SCs), and for protein gene product 9.5, a neuronal marker, to elucidate the involvement of SCs in synapse elimination. The morphological maturation of S100-immunoreactive terminal SCs at NMJs proceeded with the gradual increase in their number. The number of terminal SCs per NMJ was one or two at postnatal day (P) 7, reaching the adult number at P28, when it became three or four. Confocal laser scanning microscopic analysis of multi-innervated NMJs, whose number decreased between P7 and P14, revealed a change in the ratio between terminal SCs and axons with age. At P7, the ratio between axons and terminal SCs per NMJ was > or = 2:1, which was exactly the reverse of that in adults, while at P14 this had changed to 2:2. A structural change appeared to occur at the same time at the preterminal region, this being prior to the establishment of a 1:1 relationship between axon and SC sheath which was detected at P14, with the > or = 2:1 relationship seeming to occur at P7. Thus, synapse elimination seems to proceed, at least for one week, with the gradual loss of axons which are at different stages of maturation with respect to their spatial relationship with SCs. From our results it seems unlikely that SCs play an active role in selecting a single axon to survive.
Hirata
K
K
Department of Anatomy, Faculty of Medicine, Kyushu University, Fukuoka, Japan.
Zhou
C
C
Nakamura
K
K
Kawabuchi
M
M
D000818:Animals; D005455:Fluorescent Antibody Technique; D007834:Lasers; D018613:Microscopy, Confocal; D024510:Muscle Development; D018482:Muscle, Skeletal / Q000254:growth & development* / Q000294:innervation*; D009419:Nerve Tissue Proteins / Q000032:analysis; D009469:Neuromuscular Junction / Q000648:ultrastructure*; D051381:Rats; D017208:Rats, Wistar; D009418:S100 Proteins / Q000032:analysis; D012583:Schwann Cells / Q000502:physiology / Q000648:ultrastructure*; D013569:Synapses / Q000648:ultrastructure*; D013869:Thiolester Hydrolases / Q000032:analysis; D013997:Time Factors; D043222:Ubiquitin Thiolesterase
D009419:Nerve Tissue Proteins; D009418:S100 Proteins; D013869:Thiolester Hydrolases; D043222:Ubiquitin Thiolesterase