[Joint immobilization of L-lysine-alpha-oxidase and peroxidase on porous membrane carriers].
The goal of the present study was the development of the optimal method of co-immobilization of two enzymes: L-lysine alpha-oxidase from Trichoderma sp. and horseradish peroxidase. Commercial nitrocellulose, nylon and N+ nylon membranes were used as carriers. The immobilization was carried out either by absorbtion or by covalent binding with aldehyde groups. The aldehyde groups were attached to the surface of the carriers by UV-irradiation of membranes in the presence of p-azidotetrafluorobenzaldehyde. The optimal concentrations of reagents, enzymes and reaction conditions were found. The membranes with the co-immobilised L-lysine alpha-oxidase and peroxidase were shown to be useful for the determination of L-lysine concentrations.
Lukasheva
E V
EV
Dpt. of Biochemistry, Russian Peoples' Friendship University, Moscow, Russia.
Rubtsova
M Iu
MIu
Kovba
G V
GV
Berezov
T T
TT
Egorov
A M
AM
D000594:Amino Acid Oxidoreductases / Q000378:metabolism*; D001386:Azides / Q000737:chemistry; D001547:Benzaldehydes / Q000737:chemistry; D004800:Enzymes, Immobilized / Q000378:metabolism*; D006735:Horseradish Peroxidase / Q000378:metabolism*; D007202:Indicators and Reagents; D007700:Kinetics; D008567:Membranes, Artificial; D016062:Porosity; D014242:Trichoderma / Q000201:enzymology; D014466:Ultraviolet Rays
D001386:Azides; D001547:Benzaldehydes; D004800:Enzymes, Immobilized; D007202:Indicators and Reagents; D008567:Membranes, Artificial; C111153:p-azidotetrafluorobenzaldehyde; D006735:Horseradish Peroxidase; D000594:Amino Acid Oxidoreductases; C041123:L-lysine oxidase
Sovmestnaia immobilizatsiia L-lizin-alpha-oksidazy i peroksidazy na proistykh membrannykh nositeliakh.