Enhanced glycosylation and sulfation of secretory proteoglycans is coupled to the expression of a basic secretory protein.
We have used coexpression of a salivary basic proline-rich protein (PRP) along with a proline-rich proteoglycan (PRPg) in pituitary AtT-20 cells to examine the regulation of glycosaminoglycan (GAG) biosynthesis and the storage of these secretory products for regulated secretion. The basic PRP caused a dose-dependent increase in sulfation of PRPg and also increased the extent to which PRPg polypeptide backbones are modified by a GAG chain. The sulfation of an endogenous proteoglycan was similarly increased in the presence of basic PRP; however, other sulfated secretory products of AtT-20 cells were unaffected. These results imply that enzymes functioning in elongation and sulfation of proteoglycans are coordinately regulated and that their activities respond to a change in the milieu of the intracellular transport pathway. Analysis of the regulated secretion of both the basic PRP and PRPg has indicated that while the presence of the GAG chain improves the storage of PRPg, the presence of PRPg does not increase the storage of basic PRP. Therefore, sulfation of GAGs does not appear to be a primary factor in regulated secretory sorting.
Castle
A M
AM
Department of Cell Biology, University of Virginia Health Sciences Center, Charlottesville, Virginia 22908, USA.
Castle
J D
JD
D000818:Animals; D002460:Cell Line; D002809:Chondroitin Sulfates / Q000737:chemistry; D003594:Cytoplasmic Granules / Q000378:metabolism; D006025:Glycosaminoglycans / Q000737:chemistry / Q000378:metabolism; D006031:Glycosylation; D006497:Heparan Sulfate / Q000737:chemistry; D006863:Hydrogen-Ion Concentration; D051379:Mice; D009844:Oligosaccharides / Q000737:chemistry; D010455:Peptides / Q000737:chemistry / Q000378:metabolism*; D055232:Proline-Rich Protein Domains; D011498:Protein Precursors / Q000737:chemistry / Q000378:metabolism; D011509:Proteoglycans / Q000737:chemistry / Q000378:metabolism*; D013431:Sulfates / Q000378:metabolism
D002809:Chondroitin Sulfates; D006025:Glycosaminoglycans; D006497:Heparan Sulfate; D009844:Oligosaccharides; D010455:Peptides; D011498:Protein Precursors; D011509:Proteoglycans; D013431:Sulfates