title: In vitro studies of the interactions between platelets and amniotic membranes: a potential treatment for preterm premature rupture of the membranes.
abstract: OBJECTIVE
  Thus far there is no satisfactory treatment for preterm premature rupture of the amniotic membranes. To evaluate the use of platelets to seal the membranes, we investigated in vitro interactions between platelets and the membranes.
  
  
  METHODS
  Platelet aggregation in the presence of amnion, chorion, and amniotic fluid was quantified. Platelet adhesion to the fetal membranes was investigated by electron microscopy. In addition, a model system was designed to evaluate the capacity of platelets to seal a standardized puncture in the membranes.
  
  
  RESULTS
  Platelets aggregated in response to amnion but not to chorion or amniotic fluid. Adhesion and activation occurred on connective tissues underlying amnion and chorion but not on the amniotic epithelium. Platelets sealed a puncture in fetal membranes, and the platelet plug was visualized by electron microscopy.
  
  
  CONCLUSIONS
  Exposed connective tissue of amniotic membranes was demonstrated to trigger platelet adhesion, aggregation, and activation, and platelets were shown to seal a standardized puncture in fetal membranes. Our results indicate that platelets might be useful for treatment of ruptured membranes after needle puncture.
authors.affiliation: Department of Obstetrics, Gynecology, and Reproductive Science, Mount Sinai School of Medicine, New York, New York 10029, USA.
authors.forename: C
authors.initials: C
authors.lastname: Louis-Sylvestre
authors.forename: J H
authors.initials: JH
authors.lastname: Rand
authors.forename: R E
authors.initials: RE
authors.lastname: Gordon
authors.forename: C M
authors.initials: CM
authors.lastname: Salafia
authors.forename: R L
authors.initials: RL
authors.lastname: Berkowitz
mesh_terms: D000650:Amnion / Q000502:physiology* / Q000648:ultrastructure; D000653:Amniotic Fluid / Q000502:physiology; D001792:Blood Platelets / Q000502:physiology* / Q000648:ultrastructure; D002823:Chorion / Q000502:physiology; D005260:Female; D005322:Premature Rupture of Fetal Membranes / Q000628:therapy*; D006801:Humans; D008854:Microscopy, Electron; D008855:Microscopy, Electron, Scanning; D015539:Platelet Activation; D010973:Platelet Adhesiveness; D010974:Platelet Aggregation; D011247:Pregnancy