title: High resolution single strand conformation polymorphism analysis using formamide and ethidium bromide staining.
abstract: Single strand conformation polymorphism (SSCP) analysis using ethidium bromide can be improved by adding formamide as the denaturant. This gives higher resolution than previous SSCP methods; it had 100% sensitivity in the discrimination of 14 PCR samples from two different genes, even for a long fragment close to the upper limit of 250 base pairs. This modified procedure is a rapid, simple, safe, and yet highly sensitive method for detecting structural differences in DNA fragments.
authors.affiliation: University Department of Medicine, University of Hong Kong, Hong Kong.
authors.forename: T
authors.initials: T
authors.lastname: Xie
authors.forename: S L
authors.initials: SL
authors.lastname: Ho
authors.forename: O C
authors.initials: OC
authors.lastname: Ma
mesh_terms: D002394:Catechol O-Methyltransferase / Q000235:genetics; D004591:Electrophoresis, Polyacrylamide Gel; D004996:Ethidium; D005456:Fluorescent Dyes; D005559:Formamides; D006801:Humans; D018807:Polymorphism, Single-Stranded Conformational; D011489:Protein Denaturation; D013194:Staining and Labeling / Q000379:methods; D016159:Tumor Suppressor Protein p53 / Q000235:genetics
chemical_list: D005456:Fluorescent Dyes; D005559:Formamides; D016159:Tumor Suppressor Protein p53; C031066:formamide; D002394:Catechol O-Methyltransferase; D004996:Ethidium