NMR spectroscopic elucidation of the structure of a glycerol teichoic acid-like O-specific polysaccharide of the bacterium Hafnia alvei strain PCM 1199.
The structure of the acidic O-specific polysaccharide of a Gram-negative bacterium, H. alvei strain PCM 1199, was studied by NMR spectroscopy including two-dimensional correlation spectroscopy (COSY), total correlation spectroscopy (TOCSY), nuclear Overhauser effect spectroscopy (NOESY), 1H, 13C heteronuclear single-quantum coherence (HSQC), 1H, 13C heteronuclear multiple-bond correlation (HMBC), and one-dimensional 1H, 31P heteronuclear multiple-quantum coherence (HMQC) experiments. It was found that the polysaccharide contains D-galactose, 2-acetamido-2-deoxy-D-glucose, 4-acetamido-4,6-dideoxy-D-glucose, glycerol, and phosphate in the ratios 1:2:1:1:1, as well as O-acetyl groups in non-stoichiometric amounts. The polysaccharide is similar in structure to teichoic acids of Gram-positive bacteria and has the following structure of the repeating unit: 3)-beta-D-Galp-(1-->3)-alpha-D-GlcpNAc-(1-->3)-beta-D-Quip4NAc-(1- ->1)-Gro- 3-P-(O--> [formula: see text] beta-D-GlcpNAc [formula: see text] The O-specific polysaccharide of H. alvei PCM 1199 is structurally related to another teichoic acid-like O-specific polysaccharide of H. alvei PCM 1205 studied by us earlier.
Zatonskii
G V
GV
Zelinskii Institute of Organic Chemistry, Russian Academy of Sciences, Moscow, Russia.
Kocharova
N A
NA
Katzenellenbogen
E
E
Shashkov
A S
AS
Knirel YuA
D002240:Carbohydrate Sequence; D004755:Enterobacteriaceae / Q000737:chemistry*; D009682:Magnetic Resonance Spectroscopy; D008969:Molecular Sequence Data; D019081:O Antigens / Q000737:chemistry*; D013682:Teichoic Acids / Q000737:chemistry*
D019081:O Antigens; D013682:Teichoic Acids