title: A rapid RT-PCR based method for the detection of BCR-ABL translocation.
abstract: OBJECTIVE
  To optimise a one step reverse transcriptase polymerase chain reaction (RT-PCR) protocol for BCR-ABL chimaera detection.
  
  
  METHODS
  Compared with published RT-PCR procedures, this novel approach has at least two advantages. First, the same enzyme is used for both reverse transcription and PCR. Second, amplification of the target (BCR-ABL chimaera) and control gene (ABL) is performed simultaneously in the same tube.
  
  
  RESULTS
  On testing 40 chronic myelogenous leukaemia patients and 10 healthy donors there was a specificity for the newly developed technique. In addition, dilution experiments demonstrated that the protocol was highly sensitive.
  
  
  CONCLUSIONS
  The suggested one step PCR strategy is a simple and reliable way to reveal BCR-ABL chimaeras.
authors.affiliation: Institute of Haematology and Transfusiology, St Petersburg, Russia.
authors.lastname: Sidorova JYu
authors.forename: L B
authors.initials: LB
authors.lastname: Saltykova
authors.forename: A A
authors.initials: AA
authors.lastname: Lyschov
authors.lastname: Zaritskey AYu
authors.forename: K M
authors.initials: KM
authors.lastname: Abdulkadyrov
authors.forename: M N
authors.initials: MN
authors.lastname: Blinov
mesh_terms: D012321:DNA-Directed RNA Polymerases; D016313:Genes, abl; D006801:Humans; D015464:Leukemia, Myelogenous, Chronic, BCR-ABL Positive / Q000235:genetics*; D015513:Oncogene Proteins / Q000235:genetics*; D016133:Polymerase Chain Reaction / Q000379:methods*; D011505:Protein-Tyrosine Kinases; D011518:Proto-Oncogene Proteins; D051562:Proto-Oncogene Proteins c-bcr; D012680:Sensitivity and Specificity; D013997:Time Factors; D014178:Translocation, Genetic
chemical_list: D015513:Oncogene Proteins; D011518:Proto-Oncogene Proteins; D011505:Protein-Tyrosine Kinases; C496613:BCR protein, human; D051562:Proto-Oncogene Proteins c-bcr; D012321:DNA-Directed RNA Polymerases