MHC class I bound peptides of a colon carcinoma cell line, a Ki-ras gene-targeted progeny cell line and a B cell line.
MHC class I associated peptides on cancer cells represent potential targets for CD8+ cytotoxic T cell activity against tumor cells. We eluted the naturally bound MHC class I peptides of a colon carcinoma cell line and compared them to peptides isolated from a B cell line and a slow-growing activated Ki-ras-disrupted colon cancer cell line. While we failed to detect any significant differences in class I associated peptides due to the presence or absence of activated Ki-ras in colon cancer cell lines, the colon cancer cell lines and B cell line presented vastly different peptide repertoires in the context of HLA-A*0201 molecules.
Savoie
C J
CJ
Department of Genetics, Medical Institute of Bioregulation, Kyushu University, Fukuoka, Japan.
Kamikawaji
N
N
Sudo
T
T
Furuse
M
M
Shirasawa
S
S
Tana
T
T
Sasazuki
T
T
D001402:B-Lymphocytes / Q000276:immunology* / Q000821:virology; D016827:CD8 Antigens / Q000276:immunology; D002461:Cell Line, Transformed / Q000276:immunology; D003110:Colonic Neoplasms / Q000276:immunology*; D005805:Genes, MHC Class I / Q000276:immunology*; D011905:Genes, ras / Q000235:genetics*; D015234:HLA-A Antigens / Q000276:immunology; D004854:Herpesvirus 4, Human; D006801:Humans; D016254:Mutagenesis, Insertional; D014407:Tumor Cells, Cultured
D016827:CD8 Antigens; D015234:HLA-A Antigens