Identification of two distinct subsets of bovine gamma delta T cells with unique cell surface phenotype and tissue distribution.
We describe the characterization of two subsets of bovine gamma delta T cells having distinct cell surface phenotype and tissue distribution. One population expresses the previously described 215,000 MW WC1 antigen and is negative for the cell-surface differentiation antigens CD2, CD4, and CD8. The second population expresses CD2 and CD8 but not WC1 and appears to have a T-cell receptor (TCR) rearrangement distinct from that of the WC1+ population. The WC1- population is found in large numbers in spleen and intestine. In addition, this subset is not recognized by a number of monoclonal antibodies (mAbs) specific for TCR families that are well represented in the WC1+ population. The results indicate that the gamma delta T-cell population in cattle is considerably larger than previously described and that this population can be subdivided into two distinct subsets based on cell-surface phenotype and tissue distribution.
Machugh
N D
ND
International Livestock Research Institute Nairobi, Kenya.
Mburu
J K
JK
Carol
M J
MJ
Wyatt
C R
CR
Orden
J A
JA
Davis
W C
WC
D000818:Animals; D000954:Antigens, Surface / Q000032:analysis; D002417:Cattle / Q000276:immunology*; D005434:Flow Cytometry; D007124:Immunoenzyme Techniques; D016130:Immunophenotyping; D008198:Lymph Nodes / Q000276:immunology; D016692:Receptors, Antigen, T-Cell, gamma-delta / Q000032:analysis* / Q000235:genetics; D013154:Spleen / Q000276:immunology; D016176:T-Lymphocyte Subsets / Q000276:immunology*; D013950:Thymus Gland / Q000276:immunology; D014162:Transfection
D000954:Antigens, Surface; D016692:Receptors, Antigen, T-Cell, gamma-delta