siaD PCR ELISA for confirmation and identification of serogroup Y and W135 meningococcal infections.
Non-culture diagnosis and serogroup determination of meningococcal infection is important in contact management where vaccination may be possible. A serogroup B and C PCR ELISA assay for the non-culture diagnosis and serogroup determination has proved invaluable for enhanced epidemiological surveillance and contact management. A polymerase chain reaction assay, based on a restriction fragment length polymorphism in the meningococcal serogroup Y and W135 sialyltransferase (siaD) gene, was developed to enhance the range of non-culture diagnosis of meningococcal infection from clinical samples. The PCR assay was adapted to an ELISA format incorporating hybridisation with serogroup-specific Y and W135 oligonucleotide probes. The serogroup-specific W135 and Y PCR ELISA is a useful addition to currently available serogroup B and C assay for non-culture diagnosis of meningococcal infection and outbreak investigation.
Borrow
R
R
Manchester Public Health Laboratory, Withington Hospital, Didsbury, UK. ray@manphl.demon.co.uk
Claus
H
H
Chaudhry
U
U
Guiver
M
M
Kaczmarski
E B
EB
Frosch
M
M
Fox
A J
AJ
D004797:Enzyme-Linked Immunosorbent Assay; D005798:Genes, Bacterial; D008589:Meningococcal Infections / Q000175:diagnosis*; D009345:Neisseria meningitidis / Q000145:classification* / Q000235:genetics; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length; D012703:Serotyping; D012799:Sialyltransferases / Q000235:genetics*
D012799:Sialyltransferases