title: Refinement of pepsin digestion method for isolation of Toxoplasma gondii from infected tissues.
abstract: Modification of the acid pepsin digestion procedure to isolate Toxoplasma gondii from animal muscular tissues is described. Muscles were incubated for 60 min instead of 90 min and acid was neutralized with 1.2% (w/v) sodium bicarbonate instead by repeated washings and centrifugations.
authors.affiliation: United States Department of Agriculture, Agricultural Research Service, Livestock and Poultry Sciences Institute, Parasite Biology and Epidemiology Lab., Beltsville, MD 20705-2350, USA. jdubey@ggpl.arsusda.gov
authors.forename: J P
authors.initials: JP
authors.lastname: Dubey
mesh_terms: D000818:Animals; D051379:Mice; D018482:Muscle, Skeletal / Q000469:parasitology*; D010274:Parasitology / Q000379:methods; D010434:Pepsin A; D014122:Toxoplasma / Q000302:isolation & purification*; D014124:Toxoplasmosis, Animal / Q000175:diagnosis* / Q000473:pathology
chemical_list: D010434:Pepsin A