DNA-hydrolyzing activity of Bence Jones proteins.
Of 18 monoclonal Bence Jones proteins purified from urine of patients with multiple myeloma, 4 were found to have a DNA-nicking activity. In contrast, all Bence Jones proteins tested showed detectable amidase activity against carbobenzoxy-L-valyl-glycyl-L-arginine p-nitroanilide. No correlation between the two activities was found. Four patients excreting Bence Jones protein with the DNA-nicking activity showed somewhat severe symptoms, suggesting that this activity may be related to the progressive deterioration of clinical status.
Matsuura
K
K
Department of Biochemistry, Kinki University School of Medicine, Osaka, Japan.
Ikoma
S
S
Yoshida
K
K
Sinohara
H
H
D000328:Adult; D000368:Aged; D000581:Amidohydrolases / Q000378:metabolism; D000818:Animals; D001536:Bence Jones Protein / Q000652:urine*; D002417:Cattle; D004247:DNA / Q000378:metabolism*; D003850:Deoxyribonuclease I / Q000652:urine; D003851:Deoxyribonucleases / Q000652:urine*; D005260:Female; D006801:Humans; D006868:Hydrolysis; D008297:Male; D009101:Multiple Myeloma / Q000652:urine*; D011247:Pregnancy
D001536:Bence Jones Protein; D004247:DNA; D003851:Deoxyribonucleases; D003850:Deoxyribonuclease I; D000581:Amidohydrolases; C019665:amidase