[Suppressive effects of a plant-origin polyol, dulcitol on collagen-induced arthritis in mice].
Dulcitol was isolated chemically from Celastrus obiculatus Thumb and determined by HPLC. Effects of dulcitol were examined on collagen-induced arthritis (CIA) in DBA/1J mice. From 6 weeks after the first immunization with bovine type II collagen, dulcitol (100 mg/kg body weight/day) was administered orally to immunized mice for 9 weeks. Clinical score of CIA was improved significantly by dulcitol intervention compared with the non-treated CIA mice. Radiographic score of phalangeal destruction was also improved by dulcitol treatment. These findings suggest that dulcitol may play a role in regulation of some inflammatory responses in the present arthritis model. Significant reduction of percentage of CD4+ and CD8+ T cell subsets in the spleen leucocytes of CIA mice was observed by flow cytometry. Almost normal level of CD4+ and CD8+ T cells was observed in dulcitol-treated groups, suggesting T cell-modifying effect of dulcitol in CIA. Weight of spleen was larger in CIA mice and it was not affected by dulcitol. Anti-collagen antibody titer was increased in CIA mice, and it was not affected by dulcitol, either. Improvement of the changes of CD4+ and CD8+ T cells in the spleen by dulcitol may suggest its modulatory effect on cellular immunity.
Kobayashi
Y
Y
Department of Pharmacology, Shimane Medical University, Izumo, Japan.
Shen
J
J
Li
S H
SH
Kakizoe
E
E
Okunishi
H
H
Chen
J F
JF
D000818:Animals; D001168:Arthritis / Q000188:drug therapy* / Q000276:immunology; D016516:CD4-CD8 Ratio; D002417:Cattle; D003094:Collagen / Q000276:immunology*; D004195:Disease Models, Animal; D004376:Galactitol / Q000302:isolation & purification / Q000627:therapeutic use*; D007111:Immunity, Cellular; D008297:Male; D051379:Mice; D008811:Mice, Inbred DBA; D010946:Plants, Medicinal / Q000737:chemistry*; D013154:Spleen / Q000276:immunology
D004376:Galactitol; D003094:Collagen