Differential production of chemokines and their role in neutrophil infiltration in rat allergic inflammation.
BACKGROUND
Recently we demonstrated that activated rat macrophages produced neutrophil chemotactic factors (chemokines) including cytokine-induced neutrophil chemoattractant (CINC)-1, CINC-2alpha, CINC-2beta, CINC-3/rat macrophage inflammatory protein (MIP)-2 and rat MIP-1alpha (rMIP-1alpha).


METHODS
In the present study, by using an enzyme-linked immunosorbent assay specific for each chemokine, we determined the levels of the chemokines in the pouch fluid (inflammatory site) of the fluorescein isothiocyanate-labeled ovalbumin (FITC-OVA)-induced allergic inflammation in rats. Effects of anti-chemokine antibodies on neutrophil chemotaxis were determined in vivo and in vitro.


RESULTS
CINC-1 was the major chemokine which rapidly increased after challenge with FITC-OVA, whereas CINC-3 was a minor one, and CINC-2, CINC-3 and rMIP-1alpha increased slowly with a lag time of about 2 h. Anti-CINC-1/CINC-2 antibodies, which inhibited all the CINCs, suppressed both neutrophil infiltration in vivo and neutrophil chemotactic activity of the 8-hour pouch fluid in vitro, whereas anti-rMIP-1alpha antibody slightly suppressed the chemotaxis in vivo and in vitro.


CONCLUSIONS
Our results suggest that CINCs, especially CINC-1 and CINC-2, play an important role in the infiltration of neutrophils into the inflammatory site of FITC-OVA-induced allergic inflammation in rats.
Nakagawa
H
H
Department of Physiological Chemistry, Faculty of Pharmaceutical Sciences, Toyama Medical and Pharmaceutical University, Sugitani, Japan. nakagawa@ms.toyama-mpu.ac.jp
Ando
Y
Y
Takano
K
K
Sunada
Y
Y
D000818:Animals; D001826:Body Fluids / Q000378:metabolism; D018925:Chemokines / Q000502:physiology*; D002634:Chemotaxis, Leukocyte / Q000276:immunology*; D017449:Dermatitis, Allergic Contact / Q000209:etiology / Q000276:immunology* / Q000378:metabolism; D004797:Enzyme-Linked Immunosorbent Assay; D007114:Immunization; D007249:Inflammation / Q000276:immunology / Q000378:metabolism; D007958:Leukocyte Count; D008297:Male; D009504:Neutrophils / Q000276:immunology*; D010047:Ovalbumin / Q000031:analogs & derivatives; D011817:Rabbits; D051381:Rats; D017208:Rats, Wistar
D018925:Chemokines; C042851:fluorescein-ovalbumin; D010047:Ovalbumin