Surface phenotypes of human T cell lines generated after WGA- and PHA-stimulation.
We have reported earlier that human T lymphocytes from blood or spleens of normal donors can be propagated in long-term culture in the presence of IL-2, after stimulation either with wheat germ agglutinin (WGA) or phytohemagglutinin (PHA) and with the use of repeated restimulation with supernatants collected after 24 h of such cultures. In the present study we demonstrate that lymphoblast lines generated with this method contain predominantly the cells expressing CD3 molecule and alpha beta heterodimer of T cell receptor (TcR alpha beta). In PHA-initiated lymphoblast lines CD4-CD8+ cells prevailed, whereas in lymphoblast lines generated after WGA-stimulation various proportion of CD4+CD8-, CD+CD8- and CD4-CD8- cells were found. In some cell lines a gradual selective outgrowth of TcR alpha beta CD4+CD8- or CD4-CD8- cells was observed. These data indicate that the long-term growth potential of human normal T lymphoblasts is not restricted to a single subset and that the surface phenotypes acquired during differentiation of the cells can be retained even after more than 100 population doublings in culture.
Siwicki
J K
JK
Department of Immunology, Maria Skłodowska-Curie Memorial Cancer Center, Warsaw, Poland.
Skurzak
H
H
Steffen
J A
JA
D000945:Antigens, Differentiation, T-Lymphocyte; D017252:CD3 Complex; D015704:CD4 Antigens; D016827:CD8 Antigens; D002454:Cell Differentiation; D002460:Cell Line; D006801:Humans; D008213:Lymphocyte Activation; D010641:Phenotype; D010835:Phytohemagglutinins / Q000494:pharmacology; D016693:Receptors, Antigen, T-Cell, alpha-beta; D016176:T-Lymphocyte Subsets; D013601:T-Lymphocytes / Q000166:cytology* / Q000187:drug effects / Q000276:immunology; D014909:Wheat Germ Agglutinins / Q000494:pharmacology
D000945:Antigens, Differentiation, T-Lymphocyte; D017252:CD3 Complex; D015704:CD4 Antigens; D016827:CD8 Antigens; D010835:Phytohemagglutinins; D016693:Receptors, Antigen, T-Cell, alpha-beta; D014909:Wheat Germ Agglutinins