title: Compensation for D-glutamate auxotrophy of Escherichia coli WM335 by D-amino acid aminotransferase gene and regulation of murI expression.
abstract: D-glutamate, an indispensable component of peptidoglycans of bacteria, is provided by glutamate racemase in E. coli cells. Compensation for D-glutamate auxotrophy of E. coli WM335 cells lacking the glutamate racemase gene, murI, with the D-amino acid aminotransferase gene suggests that presence of a threshold concentration for the D-glutamate required by E. coli cells, as well as a regulation system for murI expression.
authors.affiliation: Institute for Chemical Research, Kyoto University, Kyoto-fu, Japan.
authors.forename: L
authors.initials: L
authors.lastname: Liu
authors.forename: T
authors.initials: T
authors.lastname: Yoshimura
authors.forename: K
authors.initials: K
authors.lastname: Endo
authors.forename: K
authors.initials: K
authors.lastname: Kishimoto
authors.forename: Y
authors.initials: Y
authors.lastname: Fuchikami
authors.forename: J M
authors.initials: JM
authors.lastname: Manning
authors.forename: N
authors.initials: N
authors.lastname: Esaki
authors.forename: K
authors.initials: K
authors.lastname: Soda
mesh_terms: D000410:Alanine Transaminase / Q000096:biosynthesis / Q000235:genetics / Q000378:metabolism*; D000591:Amino Acid Isomerases / Q000096:biosynthesis / Q000235:genetics / Q000378:metabolism*; D001216:Asparagine / Q000235:genetics / Q000378:metabolism; D003470:Culture Media / Q000378:metabolism / Q000494:pharmacology; D051263:D-Alanine Transaminase; D004926:Escherichia coli / Q000378:metabolism*; D015870:Gene Expression; D018698:Glutamic Acid / Q000378:metabolism* / Q000494:pharmacology; D008239:Lysine / Q000235:genetics / Q000378:metabolism; D016297:Mutagenesis, Site-Directed
chemical_list: D003470:Culture Media; D018698:Glutamic Acid; D001216:Asparagine; D000410:Alanine Transaminase; D051263:D-Alanine Transaminase; D000591:Amino Acid Isomerases; C062292:glutamate racemase; D008239:Lysine