Highly fluorescent reticulocytes after CD34+ peripheral blood progenitor cell transplantation.
Highly fluorescent reticulocyte (HFR) counts were evaluated in 13 consecutive patients affected by hematological malignancies and submitted to autologous selected CD34+ peripheral blood progenitor cell (PBPC) transplantation. Results were compared with a historical group of patients comparable for age, disease and conditioning regimen submitted to unfractionated PBPC transplantation. HFR counts of the CD34+ group declined to an undetectable level from day +4 to day +10 when they became detectable and reached 5% of total reticulocyte count by day +12. In the historical group, the nadir was identical but the recovery was faster (day +9). Total reticulocyte count > 1% was achieved at days +17 and +11, respectively. The absolute neutrophil count (ANC) recovery was identical in both groups, achieving a value > 0.5 x 10(9)/l at day +13 after reinfusion. Hence, in the historical group, HFR count gave advance notice of complete and stable hemopoietic engraftment while in the CD34+ group HFR and ANC count showed almost simultaneous recovery.
Sica
S
S
Department of Hematology, Istituto di Semeiotica Medica, Università Cattolica del Sacro Cuore, Roma, Italy.
Salutari
P
P
Laurenti
L
L
Ortu La Barbera
E
E
Zini
G
G
d'Onofrio
G
G
Serafini
R
R
Leone
G
G
D000328:Adult; D018952:Antigens, CD34 / Q000378:metabolism*; D005260:Female; D005456:Fluorescent Dyes; D006085:Graft Survival; D006410:Hematopoiesis; D018380:Hematopoietic Stem Cell Transplantation; D006689:Hodgkin Disease / Q000628:therapy; D006801:Humans; D007700:Kinetics; D008228:Lymphoma, Non-Hodgkin / Q000628:therapy; D008297:Male; D008875:Middle Aged; D009101:Multiple Myeloma / Q000628:therapy; D017701:Reticulocyte Count; D012156:Reticulocytes / Q000166:cytology* / Q000276:immunology*; D014182:Transplantation, Autologous
D018952:Antigens, CD34; D005456:Fluorescent Dyes