Production of eosinophil chemotactic factor by CD8+ T-cells in Toxocara canis-infected mice.
Production of eosinophil chemotactic factor by T-lymphocytes (ECF-L) was examined in Toxocara canis-infected mice. When spleen cells from T. canis-infected mice were cultured in serum-free RPMI1640, ECF-L production was detectable in an antigen-specific manner. The ECF-L production peaked at day 9 post-infection and then decreased. Depletion of Thy 1.2+ cells or CD8+ cells completely abrogated ECF-L production, whereas depletion of CD4+ cells did not, indicating that CD8+ T-cells are involved in the production of ECF-L. When bone marrow eosinophils obtained from T. canis-infected mice were preincubated with ECF-L, their chemotactic reactivity to parasite-derived ECFs was enhanced, whereas that of peritoneal cavity-derived eosinophils was not. Thus, ECF-L seems to be important not only as a chemoattractant but also as an activator of the chemotactic reactivity of naive eosinophils to the parasite-derived ECF in T. canis infection.
Owhashi
M
M
Faculty of Integrated Arts and Sciences, University of Tokushima, Japan. ohashi@ias.tokushima-u.ac.jp
Arita
H
H
Niwa
A
A
D000818:Animals; D000947:Antigens, Helminth; D001853:Bone Marrow; D018414:CD8-Positive T-Lymphocytes / Q000378:metabolism*; D002478:Cells, Cultured; D002631:Chemotactic Factors, Eosinophil / Q000096:biosynthesis* / Q000502:physiology; D002634:Chemotaxis, Leukocyte; D004802:Eosinophilia / Q000209:etiology / Q000276:immunology; D004804:Eosinophils / Q000502:physiology; D005260:Female; D051379:Mice; D008810:Mice, Inbred C57BL; D010529:Peritoneal Cavity; D013154:Spleen / Q000276:immunology; D017166:Toxocara canis; D014120:Toxocariasis / Q000150:complications / Q000276:immunology*
D000947:Antigens, Helminth; D002631:Chemotactic Factors, Eosinophil