title: Production of B cell differentiation factors by mouse parenchymal liver cells.
abstract: Previously we reported that most antibody secreting cells secreted IgA in the liver. Here we assessed the possibility that parenchymal liver cells (PLC) produced factors, transforming growth factor (TGF)-beta and IL-5, which participate in the differentiation of B cells to IgA-secreting cells. We showed that TGF-beta activity was present in the culture supernatant of PLC, and IL-5 activity was in the lysate of PLC. Moreover, it was confirmed that IL-5 protein produced by PLC was mainly localized in the cell membrane by histochemical staining. The findings that both TGF-beta and IL-5 were produced by PLC should provide useful information concerning the fact that IgA-secreting cells were dominant in the liver.
authors.affiliation: Department of Microbiology and Immunology, Nippon Medical School, Tokyo, Japan. megumi@nms.ac.jp
authors.forename: M
authors.initials: M
authors.lastname: Takahashi
authors.forename: E
authors.initials: E
authors.lastname: Watari
authors.forename: A
authors.initials: A
authors.lastname: Mabuchi
authors.forename: K
authors.initials: K
authors.lastname: Yokomuro
mesh_terms: D000818:Animals; D001402:B-Lymphocytes / Q000166:cytology / Q000378:metabolism*; D002454:Cell Differentiation; D002460:Cell Line; D005260:Female; D006131:Growth Inhibitors / Q000378:metabolism*; D015848:Interleukin-5 / Q000378:metabolism*; D008099:Liver / Q000166:cytology / Q000378:metabolism*; D051379:Mice; D008807:Mice, Inbred BALB C; D016212:Transforming Growth Factor beta / Q000378:metabolism*
chemical_list: D006131:Growth Inhibitors; D015848:Interleukin-5; D016212:Transforming Growth Factor beta