Identification of a transcript predicting an alternative form of sulfated glycoprotein-2 (clusterin) in rat tissues.
As a clue to understand the role of androgen in rat seminal vesicle, we have isolated several cDNA clones of androgen-responsive mRNAs. During further characterizations of these cDNAs were in progress, a distinct cDNA clone designated p116 was isolated. The sequence study suggested the expression of a transcript predicting an alternative form of Sulfated Glycoprotein-2 (SGP-2)/clusterin. RT-PCR demonstrated the androgen-dependent expression of p116 transcript in the seminal vesicles. The transcript was also detectable in the ventral prostate, the liver and the thymus. Since SGP-2 has been suggested as a classical molecular marker of apoptosis, p116 transcript newly identified in the present study might provide a useful probe to further understand the roles of SGP-2 and its related proteins during the course of apoptotic process not only in male accessory sex organs, but also in many types of cells undergoing apoptosis.
Izawa
M
M
Department of Biosignaling, School of Life Science, Faculty of Medicine, Tottori University, Yonago, Japan.
D017398:Alternative Splicing / Q000235:genetics*; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D051152:Clusterin; D006023:Glycoproteins / Q000737:chemistry / Q000235:genetics*; D008297:Male; D018832:Molecular Chaperones; D008969:Molecular Sequence Data; D009928:Organ Specificity / Q000235:genetics; D016133:Polymerase Chain Reaction; D051381:Rats; D017208:Rats, Wistar; D012669:Seminal Vesicles / Q000737:chemistry; D014158:Transcription, Genetic
C498500:Clu protein, rat; D051152:Clusterin; D006023:Glycoproteins; D018832:Molecular Chaperones