Regional specification of muscle progenitors in Drosophila: the role of the msh homeobox gene.
The somatic musculature in the abdominal hemisegments of Drosophila consists of 30 uniquely identifiable muscle fibers. Previous studies have suggested that the muscle diversity originates in a special class of myoblasts, called muscle founders, that are formed by the division of muscle progenitors. However, the mechanisms that locate and specify the muscle progenitors/founders are largely unknown. In this study, we first used a novel marker, rP298-LacZ, to chart the development of muscle progenitors/founders during the formation of distinct groups of mature muscles. We then determined the function of the muscle segment homeobox (msh) gene in myogenesis. msh encodes a homeobox-containing protein, vertebrate homologues of which are known as Msxs. We show that msh is expressed in the dorsal and lateral domains of muscle progenitors and is required for the specification of the progenitor cells. Ectopic expression of msh in the entire mesoderm inhibits the proper development of the normally msh-negative muscle progenitors in the dorsolateral domain. These results suggest that msh plays a role in regional specification of muscle progenitors/founders.
Nose
A
A
National Institute for Basic Biology, Okazaki, Japan. nose@nibb.ac.jp
Isshiki
T
T
Takeichi
M
M
D000818:Animals; D015415:Biomarkers; D004330:Drosophila / Q000196:embryology* / Q000235:genetics; D029721:Drosophila Proteins; D018507:Gene Expression Regulation, Developmental; D005801:Genes, Homeobox / Q000502:physiology*; D017930:Genes, Reporter; D018398:Homeodomain Proteins / Q000235:genetics*; D007763:Lac Operon; D008648:Mesoderm / Q000502:physiology; D009024:Morphogenesis; D018485:Muscle Fibers, Skeletal / Q000166:cytology; D009132:Muscles / Q000166:cytology / Q000196:embryology; D009154:Mutation; D009218:Myosins / Q000032:analysis; D012333:RNA, Messenger / Q000032:analysis; D011993:Recombinant Fusion Proteins / Q000032:analysis; D013234:Stem Cells / Q000737:chemistry / Q000166:cytology*
D015415:Biomarkers; C096010:Dr protein, Drosophila; D029721:Drosophila Proteins; D018398:Homeodomain Proteins; D012333:RNA, Messenger; D011993:Recombinant Fusion Proteins; D009218:Myosins