Mapping of ezrin dimerization using yeast two-hybrid screening.
Ezrin, a membrane-cytoskeleton linker protein, is involved in the recruitment of H+/K(+)-ATPase-containing tubulovesicles to the canalicular membrane during acid secretion in the parietal cell. Ezrin exists as monomers and head-to-tail dimers in vivo, and oligomerization is presumably important for activation. In this study, we mapped regions of ezrin-ezrin interaction using the yeast two-hybrid assay. We observed that the N-terminal 283 amino acids are sufficient for interaction with the carboxyl terminal 140 amino acids. The region 333.446 inhibits this association. However, the inclusion of amino acids 283-310 appears to release the inhibition. These specific interactions may play a critical role in the formation of dimerization-competent ezrin molecules.
Bhartur
S G
SG
Department of Medicine, Medical College of Georgia, Augusta, USA.
Goldenring
J R
JR
D000818:Animals; D003598:Cytoskeletal Proteins; D004268:DNA-Binding Proteins; D019281:Dimerization; D018014:Gene Transfer Techniques; D006639:Histidine / Q000235:genetics; D006801:Humans; D010295:Parietal Cells, Gastric / Q000737:chemistry; D010750:Phosphoproteins / Q000737:chemistry* / Q000235:genetics; D011817:Rabbits; D011993:Recombinant Fusion Proteins; D012441:Saccharomyces cerevisiae / Q000235:genetics; D029701:Saccharomyces cerevisiae Proteins; D017385:Sequence Homology; D014157:Transcription Factors; D001616:beta-Galactosidase / Q000235:genetics; D000099149:Ezrin
D003598:Cytoskeletal Proteins; D004268:DNA-Binding Proteins; D006639:Histidine; D010750:Phosphoproteins; D011993:Recombinant Fusion Proteins; D029701:Saccharomyces cerevisiae Proteins; D014157:Transcription Factors; D001616:beta-Galactosidase; D000099149:Ezrin; C052130:GAL4 protein, S cerevisiae