The role of CD4+ and CD8+ T-cells in host morbidity and innate resistance to angiostrongylus cantonensis in the mouse.
Strain-dependent differences in host morbidity and mortality due to Angiostrongylus cantonensis infection have been established between C57BL/6 and BALB/c mice; C57BL/6 mice show rapid worm killing with low morbidity, whereas BALB/c mice indicate slow worm killing with high morbidity and mortality. To determine the possible roles of CD4+ and CD8+ T-cells in host morbidity and innate resistance to A. cantonensis infection we treated C57BL/6 and BALB/c mice with anti-CD4 or anti-CD8 monoclonal antibody and examined the changes in host morbidity and worm-killing activity. Our study indicates that anti-CD4 antibody treatment interferes with worm killing and improves the morbidity of A. cantonensis-infected BALB/c mice, whereas anti-CD8 antibody treatment fails to improve the morbidity. Tumor necrosis factor-alpha (TNF-alpha, or cachectin) production in infected mice was not correlated with host morbidity. Anti-IL-5 monoclonal antibody treatment also failed to affect the morbidity of infected BALB/c mice, although their worm-killing activity was restrained as shown in anti-CD4-treated mice. These findings clearly indicate that the morbidity of infected BALB/c mice is regulated by some unknown CD4+ T-cell-dependent mechanism but not by an IL-5-, eosinophil-, or TNF-alpha-dependent mechanism.
Aoki
M
M
Department of Parasitology, Akita University, School of Medicine, Japan.
Sugaya
H
H
Ishida
K
K
Yoshimura
K
K
D017185:Angiostrongylus cantonensis / Q000276:immunology / Q000302:isolation & purification*; D000818:Animals; D000909:Antibodies, Helminth / Q000097:blood; D000911:Antibodies, Monoclonal / Q000276:immunology; D000947:Antigens, Helminth / Q000276:immunology; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology*; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology*; D006790:Host-Parasite Interactions; D007113:Immunity, Innate; D007074:Immunoglobulin G / Q000097:blood; D015848:Interleukin-5 / Q000276:immunology; D008213:Lymphocyte Activation; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D008819:Mice, Nude; D013045:Species Specificity; D017206:Strongylida Infections / Q000276:immunology* / Q000469:parasitology; D014409:Tumor Necrosis Factor-alpha / Q000096:biosynthesis
D000909:Antibodies, Helminth; D000911:Antibodies, Monoclonal; D000947:Antigens, Helminth; D007074:Immunoglobulin G; D015848:Interleukin-5; D014409:Tumor Necrosis Factor-alpha