PCR methods for rapid identification and characterization of Actinobacillus seminis strains.
Twenty-four isolates of Actinobacillus seminis were typed by PCR ribotyping, repetitive extragenic palindromic element (REP)-based PCR, and enterobacterial repetitive intergenic consensus (ERIC)-based PCR. Five types were distinguished by REP-PCR, and nine types were distinguished by ERIC-PCR. PCR ribotyping produced the simplest pattern and could be useful for identification of A. seminis and for its differentiation from related species. REP- and ERIC-PCR could be used for strain differentiation in epidemiological studies of A. seminis.
Appuhamy
S
S
Division of Infection and Immunity, University of Glasgow, Scotland, United Kingdom.
Coote
J G
JG
Low
J C
JC
Parton
R
R
D000188:Actinobacillus / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D000818:Animals; D015373:Bacterial Typing Techniques; D002417:Cattle; D016172:DNA Fingerprinting; D004269:DNA, Bacterial / Q000032:analysis* / Q000235:genetics; D006190:Haemophilus / Q000145:classification / Q000235:genetics; D006193:Haemophilus influenzae; D008297:Male; D016133:Polymerase Chain Reaction / Q000379:methods*; D015203:Reproducibility of Results; D012756:Sheep
D004269:DNA, Bacterial