Improving the efficiency of antisense and EGS methods.
Hammerhead ribozymes and stable stem loop structures function as inhibitors of 3'-5'-exonuclease degradation of external guide sequences (EGSs) when covalently linked to the 3'-end of EGS RNAs. This observation may be of use in improving the efficiency of gene inactivation techniques that use single-stranded RNA (e.g., antisense RNA, EGS RNA) in vivo.
Salavati
R
R
Department of Biology, Yale University, New Haven, CT 06520-81031, USA.
Altman
S
S
D001483:Base Sequence; D043211:Exodeoxyribonuclease V; D005090:Exodeoxyribonucleases; D005786:Gene Expression Regulation; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation; D016372:RNA, Antisense; D016337:RNA, Catalytic; D058727:RNA, Small Untranslated
D016372:RNA, Antisense; D016337:RNA, Catalytic; D005090:Exodeoxyribonucleases; D043211:Exodeoxyribonuclease V; D058727:RNA, Small Untranslated