Subcellular localization of Bcl-2 protein in synovial sarcoma.
The subcellular localization of Bcl-2 protein in surgically resected, fixed-frozen tissue specimens of seven tumors from six cases of synovial sarcoma and a synovial sarcoma cell line was examined using laser-scanning confocal microscopy and immunoelectron microscopy. Bcl-2 protein in synovial sarcoma cells was detected in the nuclear envelope, endoplasmic reticulum membrane, and mitochondrial circumference. Based on the finding of pre-embedding immunoelectron-microscopy observation, the distribution of Bcl-2 protein in such membranous organella was patchy. A computer-based image analysis failed to reveal any quantitative differences between the inner and the outer mitochondrial membranes. The tumorigenesis of synovial sarcoma is also discussed from the viewpoint of Bcl-2 overexpression.
Kawauchi
S
S
Second Department of Pathology, Faculty of Medicine, Kyushu University, Fukuoka, Japan.
Noguchi
S
S
Fukuda
T
T
Tsuneyoshi
M
M
D000328:Adult; D015153:Blotting, Western; D005260:Female; D019084:Fluorescent Antibody Technique, Indirect; D006801:Humans; D007150:Immunohistochemistry; D008297:Male; D018613:Microscopy, Confocal; D016253:Microscopy, Immunoelectron; D008875:Middle Aged; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000378:metabolism*; D013584:Sarcoma, Synovial / Q000378:metabolism* / Q000648:ultrastructure; D014407:Tumor Cells, Cultured
D019253:Proto-Oncogene Proteins c-bcl-2