Presence of immunoreactive endothelin-1 and atrial natriuretic peptide in human pericardial fluid.
This study was undertaken to characterize endothelin-1 (ET-1) and atrial natriuretic peptide (ANP) concentrations in human pericardial fluid, blood plasma, right atrial appendage and papillary muscle by use of specific radioimmunoassays. In patients undergoing cardiac surgery (n=16) pericardial fluid mean immunoreactive (ir-) ET-1 and ir-ANP levels were 36-fold and 4-fold higher than corresponding plasma levels, respectively. In high performance liquid chromatography (HPLC) pericardial fluid ir-ET-1 was indistinguishable from human ET-1[1-21] and the majority of pericardial fluid ir-ANP coeluted with human ANP[99-126]. Atrial tissue ir-ET-1 and ir-ANP concentrations were 17-fold and 870-fold higher than in ventricular tissue. Our present study demonstrated for the first time the presence of ir-ET-1 in the pericardial fluid in humans. Human pericardial fluid contained far the highest concentrations of ET-1 among all biological fluids tested thus far. The functions of pericardial fluid ET-1 and ANP on cardiac performance and coronary vascular tone require further investigations.
Horkay
F
F
Department of Cardiovascular Surgery, Semmelweis University of Medicine, Budapest, Hungary.
Szokodi
I
I
Selmeci
L
L
Merkely
B
B
Kékesi
V
V
Vecsey
T
T
Vuolteenaho
O
O
Ruskoaho
H
H
Juhász-Nagy
A
A
Tóth
M
M
D000328:Adult; D000368:Aged; D009320:Atrial Natriuretic Factor / Q000097:blood / Q000378:metabolism*; D001826:Body Fluids / Q000201:enzymology / Q000378:metabolism*; D002851:Chromatography, High Pressure Liquid; D019332:Endothelin-1 / Q000097:blood / Q000378:metabolism*; D005260:Female; D006325:Heart Atria / Q000378:metabolism; D006801:Humans; D008297:Male; D008875:Middle Aged; D015260:Neprilysin / Q000378:metabolism; D010496:Pericardium / Q000201:enzymology / Q000378:metabolism*; D013379:Substrate Specificity
D019332:Endothelin-1; D009320:Atrial Natriuretic Factor; D015260:Neprilysin