Thiopental induced cerebral protection during ischemia in gerbils.
Temporary interruption or reduction of cerebral blood flow during cerebrovascular surgery may rapidly result in ischemia or cerebral infarction. Thiopental has been shown to have cerebroprotective effects. However, the cerebroprotective dose of thiopental causes burst suppression of the EEG, thus this parameter cannot be used continuously for the detection of metabolic changes in the brain during thiopental anaesthesia. This study was performed in order to examine whether the multiparametric assembly (MPA), which measures energy metabolism CBF and mitochondrial (NADH) as well as extracellular ion concentrations (K+), can shed light on the mechanism of the cerebroprotective effects of thiopental. The MPA was placed on the brain of Mongolian gerbils and burst suppression of the ECoG was induced by thiopental. Cerebral ischemia was induced by occlusion of carotid arteries after burst suppression. Burst suppression of the ECoG was accompanied by a significant decrease in cerebral blood flow. In animals that received thiopental prior to ischemia, NADH increased to a lesser degree and extracellular potassium ion concentration increased to a lesser degree than in the control animals, indicating that thiopental affords protection of the brain under ischemic conditions due to improved energy metabolism. This study also demonstrates that the MPA can monitor changes occurring in the cerebral cortex even after the ECoG can no longer be used. Those findings have a significant value in the development of a new clinical monitoring device.
Zarchin
N
N
Department of Life Sciences, Bar-Ilan University, Ramat-Gan, Israel.
Guggenheimer-Furman
E
E
Meilin
S
S
Ornstein
E
E
Mayevsky
A
A
D000818:Animals; D001157:Arterial Occlusive Diseases / Q000188:drug therapy; D002545:Brain Ischemia / Q000188:drug therapy* / Q000378:metabolism; D002339:Carotid Arteries; D002540:Cerebral Cortex / Q000098:blood supply* / Q000378:metabolism; D002560:Cerebrovascular Circulation; D004569:Electroencephalography; D005110:Extracellular Space / Q000378:metabolism; D018757:GABA Modulators / Q000494:pharmacology*; D005849:Gerbillinae; D008928:Mitochondria / Q000378:metabolism; D018696:Neuroprotective Agents / Q000494:pharmacology*; D010084:Oxidation-Reduction; D011188:Potassium / Q000378:metabolism; D013874:Thiopental / Q000494:pharmacology*
D018757:GABA Modulators; D018696:Neuroprotective Agents; D013874:Thiopental; D011188:Potassium