title: Novel galanin receptor ligands.
abstract: Galanin is a neuroendocrine peptide which is 29/30 amino acids in length and is recognised by G-protein-coupled central nervous system receptors via its N-terminus. We synthesised several galanin receptor ligands and fragments around C-terminal extensions of galanin(1-13) to yield chimeric peptides with C-terminals corresponding to bioactive peptides like bradykinin(2-9), mastoparan, neuropeptide Y(25-36) or substance P(5-11), respectively. We also synthesised short galanin analogs in which galanin(1-13) was C-terminally elongated with Lys14; different pharmacologically active small molecules were then attached to the epsilon-amino group of Lys14. Several cysteine-substituted linear and ring closed analogs of galanin(1-9) and galanin(1-16) were also synthesised. The equilibrium binding constants for these peptides at hypothalamic galanin receptors were determined and found in the subnanomolar to micromolar range. The large number of peptides and their binding affinities presented here permit structure-activity relationship analysis of peptide-type ligands to galanin receptors.
authors.affiliation: Department of Neurochemistry and Neurotoxicology, Stockholm University, Sweden.
authors.forename: M
authors.initials: M
authors.lastname: Pooga
authors.forename: A
authors.initials: A
authors.lastname: Juréus
authors.forename: K
authors.initials: K
authors.lastname: Razaei
authors.forename: H
authors.initials: H
authors.lastname: Hasanvan
authors.forename: K
authors.initials: K
authors.lastname: Saar
authors.forename: K
authors.initials: K
authors.lastname: Kask
authors.forename: P
authors.initials: P
authors.lastname: Kjellén
authors.forename: T
authors.initials: T
authors.lastname: Land
authors.forename: J
authors.initials: J
authors.lastname: Halonen
authors.forename: U
authors.initials: U
authors.lastname: Mäeorg
authors.forename: A
authors.initials: A
authors.lastname: Uri
authors.forename: S
authors.initials: S
authors.lastname: Solyom
authors.forename: T
authors.initials: T
authors.lastname: Bartfai
authors.forename: U
authors.initials: U
authors.lastname: Langel
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D008024:Ligands; D008297:Male; D008969:Molecular Sequence Data; D010455:Peptides / Q000737:chemistry / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley; D044088:Receptors, Galanin; D011964:Receptors, Gastrointestinal Hormone / Q000378:metabolism*
chemical_list: D008024:Ligands; D010455:Peptides; D044088:Receptors, Galanin; D011964:Receptors, Gastrointestinal Hormone