title: Isolation and characterization of a 230 kDa protein (p230) specifically expressed in fetal brains: its involvement in neurite outgrowth from rat cerebral cortex neurons grown on monolayer of astrocytes.
abstract: By screening with monoclonal antibodies (mAbs) raised against growth cone membrane fraction from fetal porcine brains, we have identified a 230 kDa antigen, termed p230. Western blot analysis of extracts from various tissues demonstrated that p230 is specifically expressed in brains, in which its expression is temporally restricted; it was especially prominent in the embryonic and the early postnatal stage, and decreased to subdetectable levels in the adult brain. Further characterization of p230 revealed that it is a peripherally-membrane associated, cell surface protein produced by astrocytes. Neurite outgrowth of E18 rat cerebral cortex neurons cultured on a monolayer of astrocytes was significantly reduced in the presence of anti-p230 polyclonal antibody. Partial amino acid sequences of p230 purified from fetal porcine brains were highly homologous to an extracellular matrix protein, tenascin-C. These lines of evidence suggest that p230, a tenascin-C-like molecule present in fetal porcine brains, plays important roles during early brain development, particularly in growth cone guidance.
authors.affiliation: Department of Biotechnology, The University of Tokyo.
authors.forename: S
authors.initials: S
authors.lastname: Wakatsuki
authors.forename: S C
authors.initials: SC
authors.lastname: Ho
authors.forename: M
authors.initials: M
authors.lastname: Arioka
authors.forename: M
authors.initials: M
authors.lastname: Yamasaki
authors.forename: K
authors.initials: K
authors.lastname: Kitamoto
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D000911:Antibodies, Monoclonal; D001253:Astrocytes / Q000166:cytology* / Q000378:metabolism; D001921:Brain / Q000166:cytology* / Q000378:metabolism*; D001923:Brain Chemistry; D002478:Cells, Cultured; D002540:Cerebral Cortex / Q000166:cytology* / Q000378:metabolism; D004622:Embryo, Mammalian; D005326:Fetal Proteins / Q000096:biosynthesis / Q000302:isolation & purification* / Q000502:physiology*; D008565:Membrane Proteins / Q000096:biosynthesis / Q000302:isolation & purification / Q000502:physiology; D051379:Mice; D008807:Mice, Inbred BALB C; D008969:Molecular Sequence Data; D009419:Nerve Tissue Proteins / Q000096:biosynthesis / Q000302:isolation & purification* / Q000502:physiology*; D016501:Neurites / Q000378:metabolism / Q000502:physiology*; D009474:Neurons / Q000166:cytology* / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D012680:Sensitivity and Specificity; D017386:Sequence Homology, Amino Acid; D013552:Swine; D019063:Tenascin / Q000235:genetics / Q000378:metabolism
chemical_list: D000911:Antibodies, Monoclonal; D005326:Fetal Proteins; D008565:Membrane Proteins; D009419:Nerve Tissue Proteins; D019063:Tenascin