title: Efficient PCR-based random mutagenesis of sub-genic (100 bp) DNA fragments.
abstract: Here we describe a method for performing a PCR-driven random mutagenesis of 100 bp DNA fragments that yields mutations at a useful frequency. The method is a modification of the manganese ion substitution PCR technique, and neither creates 'hot-spots' nor favors transition mutations over transversions.
authors.affiliation: Department of Microbiology and Immunology, University of Tennessee, Memphis 38163, USA.
authors.forename: V
authors.initials: V
authors.lastname: Svetlov
authors.forename: T G
authors.initials: TG
authors.lastname: Cooper
mesh_terms: D004271:DNA, Fungal / Q000235:genetics*; D005800:Genes, Fungal; D016296:Mutagenesis; D016133:Polymerase Chain Reaction
chemical_list: D004271:DNA, Fungal