[Organic and inorganic blockers of potential-dependent Ca2+ channels inhibit store-dependent entry of Ca2+ into rat peritoneal macrophages].
The effect of organic and inorganic blockers of voltage-dependent Ca(2+)-channels on thapsigargin- and UTP-induced store-operated Ca(2+)-entry in Fura-2-loaded rat peritoneal macrophages was investigated. This store-dependent or "capacitative" Ca2+ influx stimulated by emptying the intracellular Ca(2+)-stores with endoplasmic Ca(2+)-ATPase inhibitor thapsigargin (0.5 microM) or purinergic agonist UTP (200 microM) is inhibited by the following pharmacological agents: two structurally distinct organic Ca(2+)-channel blockers nifedipine and verapamil; inorganic Ca(2+)-channel inhibitors Ni2+, La3+, Gd3+; nonselective cation channel blocker niflumic acid. Our data suggest that store-operated Ca2+influx channels of rat peritoneal macrophages share pharmacologic properties with L-type Ca(2+)-channels. Similar to trp-channels of Drosophila, they may resemble L-type Ca(2+)-channels lacking a voltage sensor.
Krutetskaia
Z I
ZI
Physiological Research Institute, St. Petersburg State University.
Lebedev
O E
OE
Krutetskaia
N I
NI
Petrova
T V
TV
D000818:Animals; D002118:Calcium / Q000378:metabolism*; D002121:Calcium Channel Blockers / Q000494:pharmacology*; D015220:Calcium Channels / Q000187:drug effects / Q000502:physiology; D020746:Calcium Channels, L-Type; D002478:Cells, Cultured; D016257:Fura-2; D015640:Ion Channel Gating; D017136:Ion Transport; D017737:Macrophages, Peritoneal / Q000187:drug effects* / Q000378:metabolism; D008670:Metals / Q000494:pharmacology; D009543:Nifedipine / Q000494:pharmacology; D058913:Purinergic Agonists; D051381:Rats; D019284:Thapsigargin / Q000494:pharmacology; D014544:Uridine Triphosphate / Q000494:pharmacology; D014700:Verapamil / Q000494:pharmacology
D002121:Calcium Channel Blockers; D015220:Calcium Channels; D020746:Calcium Channels, L-Type; D008670:Metals; D058913:Purinergic Agonists; D019284:Thapsigargin; D014700:Verapamil; D009543:Nifedipine; D002118:Calcium; D016257:Fura-2; D014544:Uridine Triphosphate
Organicheskie i neorganicheskie blokatory potentsialzavisimykh Ca2+- kanalov ingibiruiut depozavisimyĭ vkhod Ca2+ v peritoneal'nye makrofagi krysy.