[Modernization of the methods for detecting NADH diaphorase and glucose-6-phosphatase at the light-optical level].
A technique for NADH-diaphorase and glucose-6-phosphatase activity visualization at the light microscope level has been essentially modified by the use of a short pre-fixation of cryostat sections in a gluteraldehyde-containing fixative followed by a prolonged (18-20 h) incubation in the chilled (4-6 degrees C) standard media. Besides, for revealing NADH-diaphorase Triton X-100 is recommended to add to the incubation medium. The offered technical modifications secure a high staining intensity and specificity of both histochemical reactions tested without any substantial sophistication of the procedure.
Nevorotin
A I
AI
Research Center, St. Petersburg Medical University.
Iukina
G Iu
GIu
D000818:Animals; D015925:Cryopreservation / Q000379:methods; D008058:Dihydrolipoamide Dehydrogenase / Q000378:metabolism*; D005952:Glucose-6-Phosphatase / Q000378:metabolism*; D006651:Histocytochemistry / Q000379:methods; D007202:Indicators and Reagents; D008853:Microscopy; D013997:Time Factors; D016707:Tissue Fixation / Q000379:methods
D007202:Indicators and Reagents; D008058:Dihydrolipoamide Dehydrogenase; D005952:Glucose-6-Phosphatase
Modernizatsiia metodik vyiavleniia NADH-diaforazy i gliukozo-6-fosfatazy na svetoopticheskom urovne.