Voltage-dependent deactivation and desensitization of GABA responses in cultured murine cerebellar granule cells.
1. Electrophysiological recordings of GABAergic IPSCs and responses to applications of exogenous GABA were made from cultured murine cerebellar granule cells. In both the presence and absence of tetrodotoxin, depolarization of the postsynaptic cell consistently produced a broadening of the IPSC. This voltage-dependent change in kinetics arose entirely from a slowing of the rate of current decay. The duration of miniature IPSCs was increased by a significant but lesser amount by the GABA uptake inhibitor nipecotic acid (300 microM). 2. Five millisecond applications of 1 mM GABA elicited rapidly activating, biexponentially deactivating currents in patches derived from granule cell bodies. Deactivation of these responses was slowed by membrane depolarization. This effect arose from an increased fractional participation of the slow component of deactivation. The benzodiazepine flunitrazepam (1 microM) slowed deactivation at a holding potential of -70 mV but not at +50 mV. 3. Longer-lasting applications of GABA produced substantial biexponential macroscopic desensitization. The rate of desensitization was faster at a holding potential of +50 mV than at -70 mV. The speeding of desensitization at depolarized membrane potentials arose from an increase in the fractional contribution of the fast component of desensitization. 4. When two 5 ms, 1 mM GABA applications were made at an interstimulus latency of 150 ms, the second response was consistently smaller than the first. The depression of the second response was significantly heightened when the membrane potential was depolarized from -70 to +50 mV. 5. The degree of desensitization produced was closely linked to receptor occupancy. The rate of current deactivation was also voltage dependent when non-saturating, and therefore less desensitizing, applications of GABA were analysed. In contrast, both the GABA EC50 (approximately 30 microM) and the current activation kinetics at near EC50 agonist concentrations appeared to be voltage independent.
Mellor
J R
JR
Division of Neurobiology, MRC Laboratory of Molecular Biology, Cambridge, UK.
Randall
A D
AD
D000818:Animals; D002531:Cerebellum / Q000166:cytology / Q000187:drug effects / Q000502:physiology; D004305:Dose-Response Relationship, Drug; D004553:Electric Conductivity; D004558:Electric Stimulation; D005071:Evoked Potentials / Q000187:drug effects / Q000502:physiology; D019706:Excitatory Postsynaptic Potentials / Q000187:drug effects; D005445:Flunitrazepam / Q000037:antagonists & inhibitors / Q000494:pharmacology; D018755:GABA Agonists / Q000008:administration & dosage / Q000494:pharmacology; D018757:GABA Modulators / Q000494:pharmacology; D007473:Ion Channels / Q000187:drug effects; D008564:Membrane Potentials / Q000187:drug effects / Q000502:physiology; D051379:Mice; D008810:Mice, Inbred C57BL; D008815:Mice, Inbred Strains; D009474:Neurons / Q000187:drug effects* / Q000502:physiology*; D018079:Receptors, GABA / Q000187:drug effects / Q000502:physiology; D011963:Receptors, GABA-A / Q000502:physiology; D012680:Sensitivity and Specificity; D005680:gamma-Aminobutyric Acid / Q000502:physiology*
D018755:GABA Agonists; D018757:GABA Modulators; D007473:Ion Channels; D018079:Receptors, GABA; D011963:Receptors, GABA-A; D005680:gamma-Aminobutyric Acid; D005445:Flunitrazepam