Generation of a monoclonal antibody that induces apoptosis of hematopoietic cells.
Here, we developed anti-murine myeloid cell (NFS-60 cell) monoclonal antibodies in order to characterize the further mechanism of cell-to-cell interaction between the stromal cells and the hematopoietic progenitor cells. The established antibody, designated mAb UNF, inhibited proliferation of NFS-60 cells and induced apoptosis. Incubation of NFS-60 cells with mAb UNF initiated the cell aggregation within 3 hours. This phenomena did not require newly synthesized proteins, since the pretreatment of 1 mM cycloheximide failed to prevent the agglutination. Fifty-eight percent of mouse hematopoietic stem cells, Lin-, c-Kit+, Sca-1+ bone marrow cells, were shown to express UNF antigen. The glycolipid prepared from NFS-60 cells was specifically immunostained by mAb UNF.
Mio
H
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Tokyo Research Laboratories, Kyowa Hakko Kogyo Company, Ltd., Tokyo, Japan.
Satoh
M
M
Yokokawa
S
S
Nishio
H
H
Suda
T
T
Tamaoki
T
T
D000818:Animals; D000911:Antibodies, Monoclonal / Q000494:pharmacology*; D000941:Antigens / Q000032:analysis / Q000276:immunology; D017209:Apoptosis; D001854:Bone Marrow Cells / Q000276:immunology; D002449:Cell Aggregation / Q000187:drug effects; D002450:Cell Communication; D002455:Cell Division / Q000187:drug effects; D003513:Cycloheximide / Q000494:pharmacology; D005434:Flow Cytometry; D019084:Fluorescent Antibody Technique, Indirect; D006017:Glycolipids / Q000276:immunology; D006412:Hematopoietic Stem Cells / Q000502:physiology*; D007951:Leukemia, Myeloid / Q000276:immunology / Q000473:pathology; D051379:Mice; D011500:Protein Synthesis Inhibitors / Q000494:pharmacology; D017154:Stromal Cells / Q000502:physiology; D014407:Tumor Cells, Cultured
D000911:Antibodies, Monoclonal; D000941:Antigens; D006017:Glycolipids; D011500:Protein Synthesis Inhibitors; D003513:Cycloheximide