Expression of the cDNA and purification of P0 ribosomal protein from Lupinus luteus.
Eukaryotic ribosomal protein P0 is localized on the large ribosomal subunit at the base of the specific "stalk" structure, close to the central protuberance. Recently we have obtained a clone coding for P0 ribosomal protein from L. luteus (yellow lupin) cDNA library. Here we present its expression in E. coli cells and purification of the target protein.
Mikołajczyk
K
K
Institute of Bioorganic Chemistry, Polish Academy of Sciences, Poznan, Poland.
Barciszewski
J
J
D000595:Amino Acid Sequence; D018076:DNA, Complementary / Q000096:biosynthesis*; D004926:Escherichia coli / Q000235:genetics; D005822:Genetic Vectors; D008969:Molecular Sequence Data; D010750:Phosphoproteins / Q000096:biosynthesis / Q000235:genetics* / Q000302:isolation & purification*; D010940:Plant Proteins / Q000096:biosynthesis / Q000235:genetics* / Q000302:isolation & purification*; D012269:Ribosomal Proteins / Q000096:biosynthesis / Q000235:genetics* / Q000302:isolation & purification*; D017386:Sequence Homology, Amino Acid; D013329:Structure-Activity Relationship
D018076:DNA, Complementary; D010750:Phosphoproteins; D010940:Plant Proteins; D012269:Ribosomal Proteins; C054675:ribosomal protein P0