title: [ELISA of IgM antibodies to Thomsen-Friedenreich (TF) hapten in cancer diagnostics: comparison of data obtained with four TF-glycoconjugates].
abstract: The level of IgM antibodies to the Thomsen-Friedenreich hapten (TF) relative to the total IgM level in the blood sera of gastric and breast carcinoma patients and healthy persons was determined using enzyme-linked immunosorbent assay. The following TF glycoconjugates were tested: TF-polyacrylamide (PAA)(with 10 mol.% of TF hapten Gal beta 1-3GalNAc alpha 1-O(CH2)3NH per number of monomeric units in the polyacrylamide), TF-human serum albumin (HSA)(Gal beta 1-3GalNAc alpha 1-O-p-C6H4-HSA containing approximately 15 carbohydrate residues per HSA molecule), asialo-kappa-caseinoglycopeptide, and asialoglycophorin. The total IgM level was determined using antibodies to the mu-chain of human IgM. The statistically significant difference between cancer patients and healthy donors was revealed with two conjugates: TF-PAA and TF-HSA. In the case of TF-PPA, the sensitivity of the assay was 75-83%, and the specificity was 77%. Thus, TF-PAA is the most suitable conjugate for measuring the level of serum anti-TF-IgM antibodies.
authors.affiliation: Biolnvent International AB, Lund, Sweden.
authors.forename: E P
authors.initials: EP
authors.lastname: Smorodin
authors.forename: B
authors.initials: B
authors.lastname: Jansson
authors.forename: L
authors.initials: L
authors.lastname: Miliukhina
authors.forename: G
authors.initials: G
authors.lastname: Paaski
authors.forename: N V
authors.initials: NV
authors.lastname: Bovin
authors.forename: T V
authors.initials: TV
authors.lastname: Ovchinnikova
authors.forename: O
authors.initials: O
authors.lastname: Kurtenkov
mesh_terms: D000180:Acrylic Resins / Q000737:chemistry; D000906:Antibodies / Q000097:blood* / Q000276:immunology; D000951:Antigens, Neoplasm / Q000097:blood*; D001212:Asialoglycoproteins / Q000737:chemistry; D001943:Breast Neoplasms / Q000175:diagnosis* / Q000276:immunology; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006001:Glycoconjugates / Q000097:blood; D006241:Haptens / Q000276:immunology; D006801:Humans; D007075:Immunoglobulin M / Q000097:blood*; D012709:Serum Albumin / Q000737:chemistry; D013274:Stomach Neoplasms / Q000175:diagnosis* / Q000276:immunology; D000099166:Polyacrylamides
chemical_list: D000180:Acrylic Resins; D000906:Antibodies; D000951:Antigens, Neoplasm; D001212:Asialoglycoproteins; D006001:Glycoconjugates; D006241:Haptens; D007075:Immunoglobulin M; D012709:Serum Albumin; D000099166:Polyacrylamides; C026503:asialoglycophorin; C025991:Thomsen-Friedenreich antibodies
vernacular_title: Immunofermentnyĭ analiz IgM antitel k gaptenu Tomsena-Fridenreĭkha (TF) v onkodiagnostike. Sravnenie dannykh, poluchennykh s pomoshch'iu chetyrekh TF-glikokon"iugatov.