title: Interaction of the K channel beta subunit, Hyperkinetic, with eag family members.
abstract: Assembly of K channel alpha subunits of the Shaker (Sh) family occurs in a subfamily specific manner. It has been suggested that subfamily specificity also applies in the association of beta subunits with Sh channels (Rhodes, K. J., Keilbaugh, S. A., Barrezueta, N. X., Lopez, K. L., and Trimmer, J. S. (1995) J. Neurosci. 15, 5360-5371; Sewing, S., Roeper, J. and Pongs, O. (1996) Neuron 16, 455-463; Yu, W., Xu, J., and Li, M. (1996) Neuron 16, 441-453). Here we show that the Drosophila beta subunit homologue Hyperkinetic (Hk) associates with members of the ether go-go (eag), as well as Sh, families. Anti-EAG antibody coprecipitates EAG and HK indicating a physical association between proteins. Heterologously expressed Hk dramatically increases the amplitudes of eag currents and also affects gating and modulation by progesterone. Through their ability to interact with a range of alpha subunits, the beta subunits of voltage-gated K channels are likely to have a much broader impact on the signaling properties of neurons and muscle fibers than previously suggested.
authors.affiliation: Laboratory of Genetics, University of Wisconsin, Madison, Wisconsin 53706, USA.
authors.forename: G F
authors.initials: GF
authors.lastname: Wilson
authors.forename: Z
authors.initials: Z
authors.lastname: Wang
authors.forename: S W
authors.initials: SW
authors.lastname: Chouinard
authors.forename: L C
authors.initials: LC
authors.lastname: Griffith
authors.forename: B
authors.initials: B
authors.lastname: Ganetzky
mesh_terms: D000818:Animals; D001952:Bridged-Ring Compounds / Q000494:pharmacology; D004330:Drosophila; D029721:Drosophila Proteins; D004553:Electric Conductivity; D004594:Electrophysiology / Q000379:methods; D051638:Ether-A-Go-Go Potassium Channels; D006801:Humans; D019476:Insect Proteins / Q000378:metabolism; D015640:Ion Channel Gating; D051379:Mice; D009636:Norbornanes; D011188:Potassium / Q000378:metabolism; D015221:Potassium Channels / Q000235:genetics / Q000378:metabolism*; D011233:Precipitin Tests; D011374:Progesterone / Q000494:pharmacology; D011485:Protein Binding; D011994:Recombinant Proteins / Q000378:metabolism; D013859:Thiocarbamates; D013871:Thiones / Q000494:pharmacology; D014981:Xenopus
chemical_list: D001952:Bridged-Ring Compounds; D029721:Drosophila Proteins; D051638:Ether-A-Go-Go Potassium Channels; C094813:Hk (hyperkinetic) protein, Drosophila; D019476:Insect Proteins; C496885:Kcnh1 protein, mouse; D009636:Norbornanes; D015221:Potassium Channels; D011994:Recombinant Proteins; D013859:Thiocarbamates; D013871:Thiones; C496883:eag protein, Drosophila; D011374:Progesterone; C046498:tricyclodecane-9-yl-xanthogenate; D011188:Potassium