title: Ratio imaging: practical considerations for measuring intracellular calcium and pH in living tissue.
authors.affiliation: Department of Physiology and Biophysics, Cornell University Medical College, New York, New York 10021, USA.
authors.forename: R B
authors.initials: RB
authors.lastname: Silver
mesh_terms: D002138:Calibration; D003201:Computers; D005452:Fluoresceins / Q000737:chemistry; D005456:Fluorescent Dyes / Q000737:chemistry; D016257:Fura-2 / Q000737:chemistry; D007091:Image Processing, Computer-Assisted / Q000295:instrumentation / Q000379:methods*; D008856:Microscopy, Fluorescence / Q000295:instrumentation / Q000379:methods*
chemical_list: D005452:Fluoresceins; D005456:Fluorescent Dyes; C043829:2',7'-bis(carboxyethyl)-5(6)-carboxyfluorescein; D016257:Fura-2