Chemiluminescent measurement of oxalate in serum by detection of hydrogen peroxide generated through oxalate oxidase.
Today, chemiluminescence detection reactions have become popular in analytical biochemistry essentially due to their high sensitivity. A chemiluminescent synthetic system (luminol/porphyrin) was successfully used to measure serum oxalate by determination of hydrogen peroxide generated through oxalate oxidase (EC 1.2.3.4.). This new method is efficient and simple, highly sensitive and the results obtained in normal adult subjects are in good agreement with those of approved methods. This original application of such a chemiluminescent system allowed us to achieve a sensitive serum oxalate assay (detection limit of 0.2 mumol/L) characterized by a low serum volume (200 microL) required for analysis.
Gaulier
J M
JM
Fédération de Biochemie, Hôpital Edouard Herriot, Lyon, France.
Steghens
J P
JP
Lardet
G
G
Vallon
J J
JJ
Cochat
P
P
D000328:Adult; D006801:Humans; D006861:Hydrogen Peroxide / Q000032:analysis*; D007202:Indicators and Reagents; D008163:Luminescent Measurements; D010070:Oxalates / Q000097:blood*; D010088:Oxidoreductases; D012016:Reference Values; D012680:Sensitivity and Specificity
D007202:Indicators and Reagents; D010070:Oxalates; D006861:Hydrogen Peroxide; D010088:Oxidoreductases; C020598:oxalate oxidase