title: A short internal eliminated sequence with central conserved sequences interrupting the LA-MSC gene of the 81 locus in the hypotrichous ciliates Oxytricha fallax and O. trifallax.
abstract: IES-LA is a short Internal Eliminated Sequence interrupting LA-MSC, a protein-coding gene of the 81 locus of Oxytricha fallax and O. trifallax. IES-LA is precisely excised from the gene during development of the macronucleus. The internal eliminated sequence is bounded by CAAT ... AATG, and thereby resembles a TBE1 transposon internal eliminated sequence insertion that is grossly shortened (4.1 kbp to 52-64 bp), consistent with the hypothesis that short IESs are degenerated ancient transposons. The pattern of sequence conservation between five alleles of IES-LA shows that it differs from previously characterized classes of ciliate short IESs: while many short IESs have conserved ends and diverged centers, IES-LA is more conserved in its center and its ends are diverged. This implies a excision mechanism for IES-LA that is distinct from those for other known Oxytricha IESs.
authors.affiliation: Department of Oncological Sciences, University of Utah School of Medicine, Salt Lake City 84132, USA.
authors.forename: A
authors.initials: A
authors.lastname: Seegmiller
authors.forename: G
authors.initials: G
authors.lastname: Herrick
mesh_terms: D000818:Animals; D001483:Base Sequence; D002352:Carrier Proteins / Q000235:genetics*; D017124:Conserved Sequence; D017125:Genes, Protozoan; D008969:Molecular Sequence Data; D016813:Oxytricha / Q000235:genetics*; D015800:Protozoan Proteins; D017384:Sequence Deletion; D012689:Sequence Homology, Nucleic Acid
chemical_list: D002352:Carrier Proteins; C111483:LA MSC protein, protozoan; D015800:Protozoan Proteins