Direct detection of viable bacteria, molds, and yeasts by reverse transcriptase PCR in contaminated milk samples after heat treatment.
A fast, sensitive, and target contaminant-modulable method was developed to detect viable bacteria, molds, and yeasts after heat treatment. By reverse transcriptase PCR with elongation factor gene (EF-Tu or EF-1 alpha)-specific primers, the detection level was 10 cells ml of milk-1. The simplicity and rapidity (4 h) of the procedure suggests that this method may be easily transposable to other foods and other contaminants.
Vaitilingom
M
M
Tepral, Beverage Division Research Center of Danone Group, Strasbourg, France.
Gendre
F
F
Brignon
P
P
D000818:Animals; D001419:Bacteria / Q000302:isolation & purification*; D005658:Fungi / Q000302:isolation & purification*; D006358:Hot Temperature; D008892:Milk / Q000382:microbiology*; D010444:Peptide Elongation Factor Tu / Q000235:genetics; D016133:Polymerase Chain Reaction; D015003:Yeasts / Q000302:isolation & purification*
D010444:Peptide Elongation Factor Tu