A comparison of lycopene and canthaxanthin absorption: using the rat to study the absorption of non-provitamin A carotenoids.
The purpose of this study was to validate the use of the mesenteric lymph duct cannulated rat to study the absorption of carotenoids which do not have provitamin A activity. The absorption of two carotenoids, a hydrocarbon carotenoid (lycopene) and a xanthophyll carotenoid (canthaxanthin), were investigated. In the first experiment, lipid emulsions containing lycopene (LYC) or canthaxanthin (CTX) were continuously infused into the duodenum, and lymph was collected for analysis at 2-h intervals. The time course for absorption of carotenoids and triacylglycerol (TAG) was similar. Carotenoids and TAG reached steady-state concentrations in the lymph by 6 h. There was no evidence for a delayed release of either carotenoid from the intestine relative to TAG. During a second experiment, emulsions containing increasing concentrations of LYC or CTX (5, 10, 15, 20 mumol/L) were infused. The LYC and CTX in the lymph increased in a dose-dependent manner. The average efficiency of CTX absorption was 16% while the efficiency of LYC absorption averaged only 6%. Efficiency of carotenoid absorption was not related to concentration infused. Finally, to test whether LYC and CTX interact during absorption both were added to a lipid emulsion at equal concentrations (20 mumol/L) and infused. The carotenoids did not significantly affect each other's absorption. These results demonstrate the usefulness of the rat as an animal model to study the absorption of non-provitamin A carotenoids.
Clark
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RM
Department of Nutritional Sciences, University of Connecticut, Storrs 06269=4017, USA. rclark@canr1.cag.uconn.edu
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D000818:Animals; D001692:Biological Transport; D016644:Canthaxanthin / Q000378:metabolism*; D002338:Carotenoids / Q000378:metabolism*; D004347:Drug Interactions; D007408:Intestinal Absorption; D000077276:Lycopene; D008196:Lymph / Q000737:chemistry; D008297:Male; D051381:Rats; D014280:Triglycerides / Q000378:metabolism
D014280:Triglycerides; D002338:Carotenoids; D016644:Canthaxanthin; D000077276:Lycopene