Adenovirus-mediated human immunodeficiency virus-1 Nef expression in human monocytes/macrophages and effect of Nef on downmodulation of Fcgamma receptors and expression of monokines.
To characterize the effect of human immunodeficiency virus-1 (HIV-1) nef expression in human monocytes/macrophage (HMO) and U937 on the levels of FcgammaRs, HLA antigens, and monokines, elutriated HMOs and U937 cells were transfected with an adenovirus-mediated Nef expression system. Nef-expressing cells downmodulated FcgammaRI, FcgammaRII, and upregulated HLA class I molecules. Nef-expressing HMOs, treated with lipopolysaccharide (LPS) or phorbol 12-myristate 13-acetate (PMA), overexpressed tumor necrosis factor-alpha (TNF-alpha), interleukin-1beta (IL-1beta), and IL-10. However, IL-6 was induced by LPS and inhibited by PMA. Additionally, a subpopulation of Nef-expressing HMOs underwent apoptosis. Our data suggest that HIV-1 nef downmodulated FcgammaRs in myeloid cells in a manner similar to that previously reported for its effect on CD4+ in T cells.
De
S K
SK
Oral Infection and Immunity Branch, National Institute of Dental Research, the Laboratory of Central Nervous System Studies, National Institute of Neurological Disorders and Stroke, NIH, Bethesda, MD 20892, USA.
Venkateshan
C N
CN
Seth
P
P
Gajdusek
D C
DC
Gibbs
C J
CJ
D016475:3T3 Cells; D000256:Adenoviridae / Q000235:genetics*; D000818:Animals; D017209:Apoptosis; D015704:CD4 Antigens / Q000096:biosynthesis / Q000235:genetics; D002411:Cation Exchange Resins; D016373:DNA, Antisense / Q000235:genetics; D015536:Down-Regulation; D015967:Gene Expression Regulation, Viral; D015693:Gene Products, nef / Q000502:physiology*; D015754:Genes, nef; D005822:Genetic Vectors / Q000235:genetics*; D015497:HIV-1 / Q000235:genetics*; D006680:HLA Antigens / Q000096:biosynthesis / Q000235:genetics; D006801:Humans; D007375:Interleukin-1 / Q000096:biosynthesis / Q000235:genetics; D016753:Interleukin-10 / Q000096:biosynthesis / Q000235:genetics; D015850:Interleukin-6 / Q000096:biosynthesis / Q000235:genetics; D008055:Lipids; D008070:Lipopolysaccharides / Q000494:pharmacology; D016403:Lymphoma, Large B-Cell, Diffuse / Q000473:pathology; D008264:Macrophages / Q000378:metabolism*; D051379:Mice; D009000:Monocytes / Q000378:metabolism*; D015846:Monokines / Q000096:biosynthesis* / Q000235:genetics; D017452:Receptors, IgG / Q000096:biosynthesis* / Q000235:genetics; D015375:Receptors, Interleukin-2 / Q000096:biosynthesis / Q000235:genetics; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology; D014162:Transfection; D014407:Tumor Cells, Cultured; D014409:Tumor Necrosis Factor-alpha / Q000096:biosynthesis / Q000235:genetics; D054311:nef Gene Products, Human Immunodeficiency Virus
D015704:CD4 Antigens; D002411:Cation Exchange Resins; D016373:DNA, Antisense; D015693:Gene Products, nef; D006680:HLA Antigens; D007375:Interleukin-1; D015850:Interleukin-6; D008055:Lipids; C086724:Lipofectamine; D008070:Lipopolysaccharides; D015846:Monokines; D017452:Receptors, IgG; D015375:Receptors, Interleukin-2; D014409:Tumor Necrosis Factor-alpha; D054311:nef Gene Products, Human Immunodeficiency Virus; D016753:Interleukin-10; D013755:Tetradecanoylphorbol Acetate