E2F-6, a member of the E2F family that can behave as a transcriptional repressor.
The E2F family of proteins is required to establish the correct cell-cycle-dependent transcription of genes that direct the process of cell division. All previously identified E2F proteins can act in a similar manner; depending on whether or not they are associated with the cell cycle inhibitors the retinoblastoma protein (pRB), p107, or p130, they can either repress or activate the transcription of E2F-responsive genes. We now report the cloning and characterization of another E2F family member, E2F-6, whose structure is reminiscent of the dominant inhibitors of other transcription factor families. The dimerization and DNA binding properties of E2F-6 are similar to those of the other E2F family members. However, it is not regulated by pRB, p107, or p130, and it is unable to activate transcription. Instead, it can act to repress the transcription of E2F responsive genes by countering the activity of the other E2F complexes via a pRB-, p107-, or p130-independent mechanism.
Trimarchi
J M
JM
Center for Cancer Research, Massachusetts Institute of Technology, Building E17-517B, 77 Massachusetts Avenue, Cambridge, MA 02139, USA.
Fairchild
B
B
Verona
R
R
Moberg
K
K
Andon
N
N
Lees
J A
JA
D000595:Amino Acid Sequence; D000818:Animals; D000918:Antibody Specificity; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics; D019281:Dimerization; D050701:E2F6 Transcription Factor; D005786:Gene Expression Regulation; D017930:Genes, Reporter; D006801:Humans; D051379:Mice; D008969:Molecular Sequence Data; D005810:Multigene Family; D011485:Protein Binding; D012097:Repressor Proteins / Q000235:genetics*; D016160:Retinoblastoma Protein / Q000378:metabolism; D017386:Sequence Homology, Amino Acid; D014018:Tissue Distribution; D014157:Transcription Factors / Q000235:genetics* / Q000276:immunology / Q000378:metabolism
D018076:DNA, Complementary; D050701:E2F6 Transcription Factor; C491744:E2F6 protein, human; C111236:E2f6 protein, mouse; D012097:Repressor Proteins; D016160:Retinoblastoma Protein; D014157:Transcription Factors