Mutations at residues Tyr771 and Tyr783 of phospholipase C-gamma1 have different effects on cell actin-cytoskeleton organization and cell proliferation in CCL-39 cells.
Currently a central question that remains unresolved is the potential importance of phospholipase C (PLC)-gamma1 in cell mitogenesis. In this study, we introduced wild-type PLC-gamma1 plasmid and mutants Y771F and Y783F into CCL-39 fibroblasts and investigated their effect on host cell functions. To our surprise, Y771F and Y783F plasmids appeared to have opposite effects on CCL-39 cell actin-cytoskeleton organization and cell proliferation. Y771F transfectants increased cell proliferation by two-fold. Y783F transfectants showed much thicker actin filaments and decreased cell growth rate by 50%. These results suggest that PLC-gamma1 mutations have an essential impact on cell mitogenesis.
Pei
Z D
ZD
Department of Biochemistry and Biophysics, School of Medicine, University of Pennsylvania, Philadelphia 19104, USA.
Williamson
J R
JR
D000199:Actins / Q000502:physiology*; D000818:Animals; D017871:Calcium-Calmodulin-Dependent Protein Kinases / Q000378:metabolism; D002455:Cell Division; D002460:Cell Line; D006224:Cricetinae; D003412:Cricetulus; D003599:Cytoskeleton / Q000502:physiology*; D005347:Fibroblasts; D007527:Isoenzymes / Q000096:biosynthesis / Q000235:genetics / Q000378:metabolism*; D019950:Mitogen-Activated Protein Kinase 1; D009024:Morphogenesis; D009154:Mutation; D051966:Phospholipase C gamma; D019000:Phosphotyrosine / Q000378:metabolism; D010738:Type C Phospholipases / Q000096:biosynthesis / Q000235:genetics / Q000378:metabolism*; D014443:Tyrosine / Q000235:genetics / Q000378:metabolism*
D000199:Actins; D007527:Isoenzymes; D019000:Phosphotyrosine; D014443:Tyrosine; D017871:Calcium-Calmodulin-Dependent Protein Kinases; D019950:Mitogen-Activated Protein Kinase 1; D010738:Type C Phospholipases; D051966:Phospholipase C gamma