title: Anti-CagA antibodies are associated with atrophic gastritis in a population at high gastric cancer risk: a morphometric study by computerized image analysis.
abstract: BACKGROUND
  CagA-positive Helicobacter pylori strains appear to increase the risk for atrophic gastritis.
  
  
  OBJECTIVE
  To verify the association between CagA status and atrophic gastritis in the general population by means of computerized image analysis.
  
  
  METHODS
  Forty-five subjects were chosen out of a representative sample of a population at high gastric cancer risk.
  
  
  METHODS
  Helicobacter pylori status was assessed by IgG ELISA, rapid urease test and histology. Serum anti-CagA antibodies were detected by western blotting. Subjects were subdivided into 3 groups: 15 subjects Helicobacter pylori positive CagA-positive, 15 Helicobacter pylori positive CagA-negative and 15 controls Helicobacter pylori negative. Biopsies were studied using the Sydney System score. A computerized image analysis was used to count inflammatory cells in the lamina propria and to measure the area of the gastric glands.
  
  
  RESULTS
  Anti-CagA antibodies were associated with reduction of gland area (126,671 +/- 81,032 mu 2/mm2 vs 231,384 +/- 54,159; p = 0.0001), with increasing both of polymorphonuclear cells (426 +/- 238 cell/mm2 vs 136 +/- 69; p = 0.0001) and mononuclear cells (8675 +/- 1304 cell/mm2 vs 7141 +/- 1230; p = 0.003).
  
  
  CONCLUSIONS
  The association of anti-CagA antibodies with a high grade of gastric atrophy further supports the hypothesis that Helicobacter pylori CagA-positive strains can promote the multifactorial process of gastric carcinogenesis.
authors.affiliation: Department of Internal Medicine, University of Bologna, Italy.
authors.forename: F
authors.initials: F
authors.lastname: Bonvicini
authors.forename: L
authors.initials: L
authors.lastname: Baldini
authors.forename: S
authors.initials: S
authors.lastname: Pretolani
authors.forename: N
authors.initials: N
authors.lastname: Figura
authors.forename: G
authors.initials: G
authors.lastname: Epifanio
authors.forename: A
authors.initials: A
authors.lastname: Armuzzi
authors.forename: F
authors.initials: F
authors.lastname: Miglio
authors.forename: G
authors.initials: G
authors.lastname: Gasbarrini
mesh_terms: D000328:Adult; D000368:Aged; D000907:Antibodies, Bacterial / Q000032:analysis*; D000942:Antigens, Bacterial / Q000276:immunology*; D001426:Bacterial Proteins / Q000276:immunology*; D005260:Female; D005753:Gastric Mucosa / Q000473:pathology*; D005757:Gastritis, Atrophic / Q000150:complications / Q000382:microbiology / Q000473:pathology*; D016480:Helicobacter pylori / Q000276:immunology*; D006801:Humans; D007091:Image Processing, Computer-Assisted; D008297:Male; D008875:Middle Aged; D012307:Risk Factors; D013274:Stomach Neoplasms / Q000209:etiology*
chemical_list: D000907:Antibodies, Bacterial; D000942:Antigens, Bacterial; D001426:Bacterial Proteins; C080866:cagA protein, Helicobacter pylori