Polyamine depletion protects HL-60 cells from 2-deoxy-D-ribose-induced apoptosis.
We investigated the involvement of natural polyamines in HL-60 cell death triggered by exposure to 2-deoxy-D-ribose (dRib). In contrast to previous studies, exogenous polyamines failed to protect HL-60 cells against apoptosis caused by dRib. Moreover, in our experimental conditions, depletion of intracellular levels of putrescine and spermidine by alpha-difluoromethylornithine (DFMO) delayed the onset of apoptosis by at least a day or so. Exogenous polyamines reversed the beneficial effect of DFMO and restored the apoptotic levels observed in dRib-treated cells. We suggested that polyamines, especially putrescine and spermidine, act as facilitating factors in the induction of apoptosis triggered by dRib in HL-60 cells.
Monti
M G
MG
Ghiaroni
S
S
Pernecco
L
L
Barbieri
D
D
Marverti
G
G
Franceschi
C
C
D017209:Apoptosis / Q000187:drug effects*; D053938:DNA Fragmentation; D003855:Deoxyribose / Q000494:pharmacology*; D000518:Eflornithine / Q000494:pharmacology; D004791:Enzyme Inhibitors / Q000494:pharmacology; D018922:HL-60 Cells; D006801:Humans; D065108:Ornithine Decarboxylase Inhibitors; D011700:Putrescine / Q000378:metabolism*; D013095:Spermidine / Q000378:metabolism*
D004791:Enzyme Inhibitors; D065108:Ornithine Decarboxylase Inhibitors; D003855:Deoxyribose; D013095:Spermidine; D011700:Putrescine; D000518:Eflornithine