title: Effect of artificial endolymph injection into the cochlear duct on the endocochlear potential.
abstract: We investigated the effect of acute endolymphatic hydrops on the positive endocochlear potential (+EP) and negative endocochlear potential (-EP). The +EP was measured in guinea pigs during injection (without outlet) and perfusion (with outlet) of artificial endolymph into the cochlear duct. The -EP was measured during anoxia after the injection or the perfusion had finished. Injection of artificial endolymph produced a slight transient increase in the +EP, and a significant decrease in the magnitude of the -EP. Chronic endolymphatic hydrops produces both +EP and -EP decrease. The +EP decrease in chronic endolymphatic hydrops may cause the chronic change of the inner ear. The +EP increase in acute endolymphatic hydrops may be caused by a shift of the basilar membrane. However, the mechanism of the 'transient' +EP increase is not clear. The -EP decrease was not observed in animals whose cochlear duct was perfused with artificial endolymph. Therefore, the artificial endolymph itself did not cause the decrease in magnitude of the -EP. Dysfunction of the hair cells is a possible explanation for the -EP decrease but the mechanism of such a decrease is not clear in the present study. However, the results of this study support the notion that small increases in endolymphatic pressure below the resolution of recent measurements (DeMott and Salt, 1997) can lead directly to a reduction of the -EP during hydrops. The animal model described here can eliminate the chronic effect of hydrops, therefore, this model is useful for investigations into the effect of hydrops itself on the inner ear and the mechanism of hearing loss in Ménière's disease.
authors.affiliation: Department of Otolaryngology, Kochi Medical School, Nankoku, Japan.
authors.forename: A
authors.initials: A
authors.lastname: Kakigi
authors.forename: T
authors.initials: T
authors.lastname: Takeda
mesh_terms: D000200:Action Potentials; D000818:Animals; D003051:Cochlea / Q000502:physiology*; D003053:Cochlear Duct / Q000502:physiology*; D003055:Cochlear Microphonic Potentials; D004195:Disease Models, Animal; D004594:Electrophysiology; D004710:Endolymph / Q000502:physiology*; D018159:Endolymphatic Hydrops / Q000209:etiology / Q000473:pathology / Q000503:physiopathology; D006168:Guinea Pigs; D008564:Membrane Potentials; D008575:Meniere Disease / Q000503:physiopathology