Molecular cloning and characterization of a cDNA encoding asparagine synthetase from soybean (Glycine max L.) cell cultures.
A cDNA encoding glutamine-dependent asparagine synthetase was isolated from dark-adapted Glycine max cell culture. The deduced amino acid sequence showed 76-89% identity with other plant sequences. The gene for asparagine synthetase is expressed predominantly in shoots as compared to roots of etiolated plants and the level of expression decreases following light treatment, suggesting that the gene expression is down-regulated by light.
Yamagata
H
H
Laboratory of Biochemistry, Faculty of Agriculture, Kobe University, Japan.
Nakajima
A
A
Bowler
C
C
Iwasaki
T
T
D000595:Amino Acid Sequence; D001483:Base Sequence; D019733:Carbon-Nitrogen Ligases with Glutamine as Amide-N-Donor / Q000096:biosynthesis / Q000235:genetics*; D002478:Cells, Cultured; D003001:Cloning, Molecular; D018076:DNA, Complementary; D008969:Molecular Sequence Data; D012333:RNA, Messenger; D013025:Glycine max / Q000201:enzymology*
D018076:DNA, Complementary; D012333:RNA, Messenger; D019733:Carbon-Nitrogen Ligases with Glutamine as Amide-N-Donor; C019742:asparagine synthetase (glutamine-hydrolyzing)