1,4-Dihydroxynonene mercapturic acid, the major end metabolite of exogenous 4-hydroxy-2-nonenal, is a physiological component of rat and human urine.
In the present study 1,4-dihydroxynonene mercapturic acid (DHN-MA), previously shown to be the major urinary metabolite of 4-hydroxy-2-nonenal (HNE) administered to the rat, was characterized and determined to be a normal constituent of rat and human urine. DHN-MA was excreted as a mixture of at least two stereoisomers as determined by ion trap LC-MS/MS/MS after solid-phase extraction and HPLC purification. The 24-h urinary excretion of this compound was about 10 ng and 5 microg for rat and human, respectively. This end metabolite of the lipid peroxidation product HNE could represent a specific and noninvasive biomarker.
Alary
J
J
Laboratoire des Xénobiotiques, INRA, Toulouse, France.
Debrauwer
L
L
Fernandez
Y
Y
Cravedi
J P
JP
Rao
D
D
Bories
G
G
D000328:Adult; D000447:Aldehydes / Q000032:analysis / Q000378:metabolism / Q000652:urine*; D000818:Animals; D015415:Biomarkers; D002851:Chromatography, High Pressure Liquid; D015853:Cysteine Proteinase Inhibitors / Q000032:analysis / Q000378:metabolism / Q000652:urine*; D006801:Humans; D015227:Lipid Peroxidation; D008297:Male; D013058:Mass Spectrometry; D008875:Middle Aged; D051381:Rats; D013237:Stereoisomerism
D000447:Aldehydes; D015415:Biomarkers; D015853:Cysteine Proteinase Inhibitors; C027576:4-hydroxy-2-nonenal