Ischemia induces a reduction in the content of ulinastatin-like substance in the murine hippocampus.
Effects of ischemia on the content of a ulinastatin (UT)-like substance in the murine cerebral cortex and hippocampus were studied. At 24 h post-ischemia, a significant (p < 0.05) decrease in the content of UT-like substance in the hippocampus but not the cerebral cortex and a concurrent increase in the activity of micro-calpain were observed. In in vitro experiments, a decrease was registered in the content of UT-like substance in the hippocampus in the presence of calcium. This decrease was inhibited by both EDTA and calpastatin treatments. These results implicate the destruction of UT-like substance by micro-calpain in the ischemic hippocampus.
Shikimi
T
T
Department of Pharmacology, Shimane Medical University, Enya, Izumo, Japan.
Gonda
T
T
Takahashi
M
M
Kaneto
H
H
Okunishi
H
H
Takaori
S
S
D000818:Animals; D002122:Calcium Chloride / Q000494:pharmacology; D002135:Calcium-Binding Proteins / Q000494:pharmacology; D002154:Calpain / Q000378:metabolism; D002540:Cerebral Cortex / Q000187:drug effects / Q000378:metabolism; D004492:Edetic Acid / Q000494:pharmacology; D006023:Glycoproteins / Q000378:metabolism*; D006624:Hippocampus / Q000187:drug effects / Q000378:metabolism*; D002546:Ischemic Attack, Transient / Q000378:metabolism*; D007700:Kinetics; D008297:Male; D051379:Mice; D008813:Mice, Inbred ICR; D014361:Trypsin Inhibitors / Q000378:metabolism*
D002135:Calcium-Binding Proteins; D006023:Glycoproteins; D014361:Trypsin Inhibitors; C033668:calpastatin; D004492:Edetic Acid; D002154:Calpain; D002122:Calcium Chloride; C028665:urinastatin