title: Expression of the cDNA and purification of P0 ribosomal protein from Lupinus luteus.
abstract: Eukaryotic ribosomal protein P0 is localized on the large ribosomal subunit at the base of the specific "stalk" structure, close to the central protuberance. Recently we have obtained a clone coding for P0 ribosomal protein from L. luteus (yellow lupin) cDNA library. Here we present its expression in E. coli cells and purification of the target protein.
authors.affiliation: Institute of Bioorganic Chemistry, Polish Academy of Sciences, Poznan, Poland.
authors.forename: K
authors.initials: K
authors.lastname: Mikołajczyk
authors.forename: J
authors.initials: J
authors.lastname: Barciszewski
mesh_terms: D000595:Amino Acid Sequence; D018076:DNA, Complementary / Q000096:biosynthesis*; D004926:Escherichia coli / Q000235:genetics; D005822:Genetic Vectors; D008969:Molecular Sequence Data; D010750:Phosphoproteins / Q000096:biosynthesis / Q000235:genetics* / Q000302:isolation & purification*; D010940:Plant Proteins / Q000096:biosynthesis / Q000235:genetics* / Q000302:isolation & purification*; D012269:Ribosomal Proteins / Q000096:biosynthesis / Q000235:genetics* / Q000302:isolation & purification*; D017386:Sequence Homology, Amino Acid; D013329:Structure-Activity Relationship
chemical_list: D018076:DNA, Complementary; D010750:Phosphoproteins; D010940:Plant Proteins; D012269:Ribosomal Proteins; C054675:ribosomal protein P0