Production of recombinant virus-like particles from human papillomavirus types 6 and 11, and study of serological reactivities between HPV 6, 11, 16 and 45 by ELISA: implications for papillomavirus prevention and detection.
The L1 major capsid proteins of human papillomaviruses types 6 and 11 were expressed in insect cells using recombinant baculoviruses. These L1 proteins were shown to self-assemble into virus-like particles resembling papillomavirus virions as previously observed for HPV 16 and 45. However, we observed variations in the yield of virus-like particles among the four genotypes investigated. This suggests that more than one strain of each genotype has to be investigated to obtain the high level of virus-like particle production necessary to develop HPV vaccines or serological tests. Cross-reactivities between HPV 6, 11, 16 and 45 were studied using polyclonal and monoclonal antibodies to virus-like particles, L1 proteins and synthetic peptides. Although antisera react strongly against homologous virus-like particles, there is evidence of some cross-reactivity. This could be one of the explanations for the fact that antibodies to one genotype are detected in individuals infected with another genotype. This study also identified a linear epitope recognized by anti-HPV 16 virus-like particle sera.
Touzé
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A
Laboratoire d'Immunologie des Maladies Infectieuses, Faculté des Sciences Pharmaceutiques Philippe Maupas, Tours, France.
Dupuy
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C
Mahé
D
D
Sizaret
P Y
PY
Coursaget
P
P
D000595:Amino Acid Sequence; D000818:Animals; D000911:Antibodies, Monoclonal; D003429:Cross Reactions; D004797:Enzyme-Linked Immunosorbent Assay; D005814:Genes, Viral / Q000235:genetics; D005838:Genotype; D008969:Molecular Sequence Data; D027383:Papillomaviridae / Q000235:genetics* / Q000276:immunology / Q000302:isolation & purification; D030361:Papillomavirus Infections / Q000175:diagnosis* / Q000517:prevention & control*; D018411:Spodoptera; D014412:Tumor Virus Infections / Q000175:diagnosis* / Q000517:prevention & control*; D014759:Viral Envelope Proteins / Q000235:genetics / Q000276:immunology; D014760:Viral Fusion Proteins / Q000235:genetics / Q000276:immunology; D014771:Virion / Q000302:isolation & purification
D000911:Antibodies, Monoclonal; D014759:Viral Envelope Proteins; D014760:Viral Fusion Proteins; C076634:NEG envelope glycoprotein, Negishi virus