title: Endocrine and metabolic responses in sheep during halothane and pentobarbitone anaesthesia with dobutamine infusion.
abstract: The study investigated the stimulus to pituitary-adrenocortical activity (PACA) during halothane anaesthesia. Groups of six sheep were anaesthetized with thiopentone/halothane (TH group), acepromazine/thiopentone/halothane (ATH group) or pentobarbitone (P group). Dobutamine was infused in the TH and ATH groups to prevent hypotension (0.3-1.4 micrograms/kg/min) and in the P group at 0.05 microgram/kg/min. Pulse rate, arterial blood gases and pressure (ABP) were measured and sequential blood samples taken for assay of cortisol, adrenocorticotrophic hormone (ACTH), arginine vasopressin (AVP), glucose and lactate. Pulse rate increased in all groups. Arterial blood pressure decreased by 13% in TH, by 24% in ATH and remained stable in P. All three groups developed hypercapnia and acidosis but were well oxygenated. Cortisol increased in all groups; with ATH the sevenfold rise occurred earlier than with either TH (sixfold rise) or P (fivefold rise). Adrenocorticotrophic hormone changes were as for cortisol but AVP increases were not consistent. Glucose and lactate were stable, but lactate was lowest with ATH. Dobutamine infusion failed to prevent hypotension during halothane anaesthesia and PACA appeared proportional to the hypotension. Dobutamine may have stimulated ACTH and cortisol release after 120 min. Halothane-induced hypotension may cause adrenocortical activity but a direct effect of halothane cannot be ruled out.
authors.affiliation: Physiological Laboratory, University of Cambridge, UK.
authors.forename: P M
authors.initials: PM
authors.lastname: Taylor
mesh_terms: D000075:Acepromazine / Q000008:administration & dosage / Q000494:pharmacology; D000324:Adrenocorticotropic Hormone / Q000097:blood; D000758:Anesthesia / Q000379:methods / Q000662:veterinary*; D000818:Animals; D001127:Arginine Vasopressin / Q000097:blood; D001786:Blood Glucose / Q000187:drug effects / Q000378:metabolism; D001794:Blood Pressure / Q000187:drug effects*; D002245:Carbon Dioxide / Q000097:blood; D004280:Dobutamine / Q000008:administration & dosage / Q000494:pharmacology*; D005260:Female; D006221:Halothane / Q000008:administration & dosage / Q000494:pharmacology*; D006339:Heart Rate / Q000187:drug effects*; D006728:Hormones / Q000097:blood* / Q000378:metabolism; D006854:Hydrocortisone / Q000097:blood; D007262:Infusions, Intravenous; D007773:Lactates / Q000097:blood; D010100:Oxygen / Q000097:blood; D010424:Pentobarbital / Q000008:administration & dosage / Q000494:pharmacology*; D011674:Pulse; D012756:Sheep; D013997:Time Factors
chemical_list: D001786:Blood Glucose; D006728:Hormones; D007773:Lactates; D001127:Arginine Vasopressin; D002245:Carbon Dioxide; D004280:Dobutamine; D000075:Acepromazine; D000324:Adrenocorticotropic Hormone; D010424:Pentobarbital; D010100:Oxygen; D006221:Halothane; D006854:Hydrocortisone