Separate and variably shaped chromosome arm domains are disclosed by chromosome arm painting in human cell nuclei.
Fluorescence in situ hybridization (FISH) with microdissection probes from human chromosomes 3 and 6 was applied to visualize arm and subregional band domains in human amniotic fluid cell nuclei. Confocal laser scanning microscopy and quantitative three-dimensional image analysis showed a pronounced variability of p- and q-arm domain arrangements and shapes. Apparent intermingling of neighbouring arm domains was limited to the domain surface. Three-dimensional distance measurements with pter and qter probes supported a high variability of chromosome territory folding.
Dietzel
S
S
Institute of Human Genetics, University of Heidelberg, Germany. dietzel@wotan.iwr.uni-heidelberg.de
Jauch
A
A
Kienle
D
D
Qu
G
G
Holtgreve-Grez
H
H
Eils
R
R
Münkel
C
C
Bittner
M
M
Meltzer
P S
PS
Trent
J M
JM
Cremer
T
T
D000653:Amniotic Fluid / Q000166:cytology; D002478:Cells, Cultured; D002893:Chromosomes, Human, Pair 3; D002896:Chromosomes, Human, Pair 6; D015342:DNA Probes; D005260:Female; D006801:Humans; D007091:Image Processing, Computer-Assisted; D017404:In Situ Hybridization, Fluorescence / Q000379:methods*; D007399:Interphase; D008214:Lymphocytes; D008297:Male; D008677:Metaphase; D018613:Microscopy, Confocal
D015342:DNA Probes