Evaluation of PCR for diagnosis of Bordetella pertussis and Bordetella parapertussis infections.
PCR, using primers Plp1 and Plp2, was evaluated for the detection of DNA from Bordetella pertussis in bacterial strains and in nasopharyngeal samples from patients with a cough lasting at least 7 days. The assay could detect DNA from 6 CFU of B. pertussis/10 microl of sample. Results of the PCR assay were compared with those of cultures, a determination of serum antibodies against pertussis toxin and filamentous hemagglutinin, and a clinical evaluation of 2,442 coughing episodes. The overall sensitivity of PCR was 65% (623 of 956), which was higher than the sensitivity of cultures (58%) (P < 0.001). Factors influencing the sensitivity of PCR were the interval between the onset of symptoms and sampling and the vaccination status of the patient. The specificity of PCR was 98% (1,451 of 1,486). The positive and negative predictive values were 95 and 81%, respectively. Parapertussis PCR, using primers BPPA and BPPZ, was positive in 11 of 18 culture-positive cases and was confirmed by serology in another 4 cases. In conclusion, PCR is a valuable complement to cultures and can probably replace cultures for diagnosis of B. pertussis and Bordetella parapertussis infections.
Lind-Brandberg
L
L
Department of Clinical Bacteriology, Göteborg University, Sweden.
Welinder-Olsson
C
C
Lagergård
T
T
Taranger
J
J
Trollfors
B
B
Zackrisson
G
G
D000907:Antibodies, Bacterial / Q000097:blood; D001884:Bordetella / Q000235:genetics / Q000302:isolation & purification*; D001885:Bordetella Infections / Q000175:diagnosis* / Q000517:prevention & control; D001886:Bordetella pertussis / Q000235:genetics / Q000302:isolation & purification*; D017931:DNA Primers; D004269:DNA, Bacterial / Q000032:analysis*; D015721:Diphtheria-Tetanus-Pertussis Vaccine; D004797:Enzyme-Linked Immunosorbent Assay; D005069:Evaluation Studies as Topic; D006801:Humans; D007223:Infant; D009305:Nasopharynx / Q000382:microbiology; D016133:Polymerase Chain Reaction; D011237:Predictive Value of Tests; D012680:Sensitivity and Specificity; D014121:Toxoids / Q000276:immunology; D014917:Whooping Cough / Q000175:diagnosis* / Q000517:prevention & control
D000907:Antibodies, Bacterial; D017931:DNA Primers; D004269:DNA, Bacterial; D015721:Diphtheria-Tetanus-Pertussis Vaccine; D014121:Toxoids; C051093:pertussis toxoid