Prolactin-induced expression of TATA-less cyclin D3 gene is mediated by Sp1 and AP2.
The TATA-less cyclin D3 gene is stimulated by prolactin (PRL) in rat Nb2 T-lymphocytes. DNA mobility shift assay and luciferase transfection analysis revealed that the gene has a potential promoter region, two enhancer domanins and a silencer region. The promoter region possessed three Sp1 sites and two AP2 sites, and both enhancer regions had one AP2 binding site each. Analysis using oligonucleotide probes and purified AP2 protein has suggested that the expression of rat cyclin D3 gene is promoted by Sp1 and AP2 factors.
Yang
M
M
Department of Biochemistry, Mie University Faculty of Medicine, Japan.
Nomura
H
H
Hu
Y
Y
Kaneko
S
S
Kaneko
H
H
Tanaka
M
M
Nakashima
K
K
D000818:Animals; D001483:Base Sequence; D001667:Binding, Competitive / Q000235:genetics; D056743:Cyclin D3; D016213:Cyclins / Q000096:biosynthesis / Q000187:drug effects / Q000235:genetics*; D004268:DNA-Binding Proteins / Q000502:physiology*; D004742:Enhancer Elements, Genetic / Q000187:drug effects; D005786:Gene Expression Regulation / Q000187:drug effects*; D016399:Lymphoma, T-Cell; D008969:Molecular Sequence Data; D009687:Nuclear Proteins / Q000235:genetics / Q000378:metabolism; D011388:Prolactin / Q000494:pharmacology*; D011401:Promoter Regions, Genetic / Q000187:drug effects; D011485:Protein Binding / Q000235:genetics; D051381:Rats; D012097:Repressor Proteins / Q000235:genetics / Q000378:metabolism; D016329:Sp1 Transcription Factor / Q000502:physiology*; D016385:TATA Box; D050656:Transcription Factor AP-2; D014157:Transcription Factors / Q000502:physiology*; D014407:Tumor Cells, Cultured
C534698:Ccnd3 protein, rat; D056743:Cyclin D3; D016213:Cyclins; D004268:DNA-Binding Proteins; D009687:Nuclear Proteins; D012097:Repressor Proteins; D016329:Sp1 Transcription Factor; D050656:Transcription Factor AP-2; D014157:Transcription Factors; D011388:Prolactin