title: Role of Arg-gingipain A in virulence of Porphyromonas gingivalis.
abstract: In order to access the role of the Porphyromonas gingivalis Arg-gingipain proteases in the virulence of this organism, a mutant defective in the rgpA gene was constructed in strain 381. This mutant, MT10, displayed only 40% of the Arg-specific cysteine protease activity of the wild-type strain. In addition, MT10, as well as the recently characterized protease mutant G-102, which is defective in the rgpB gene, displayed reduced self-aggregation, hemagglutination, and the ability to bind to immobilized type I collagen compared to levels of the wild-type parent. However, unlike mutant G-102, the rgpA mutant displayed increased binding to epithelial cells relative to that of the parental organism. Mutant MT10 also did not express detectable levels of the FimA protein as assessed by both Western and Northern blotting or fimbriae visible by electron microscopy of the cells. Furthermore, the ability of MT10 to degrade rat tail collagen fibers when it was cultured at 37 degrees C was markedly attenuated compared to that of strain 381. These results suggest that Arg-gingipain A may play a significant role in the pathogenicity of P. gingivalis by altering the colonization and toxic properties of the organism.
authors.affiliation: Department of Oral Biology, State University of New York, Buffalo 14214, USA.
authors.forename: M
authors.initials: M
authors.lastname: Tokuda
authors.forename: T
authors.initials: T
authors.lastname: Karunakaran
authors.forename: M
authors.initials: M
authors.lastname: Duncan
authors.forename: N
authors.initials: N
authors.lastname: Hamada
authors.forename: H
authors.initials: H
authors.lastname: Kuramitsu
mesh_terms: D018829:Adhesins, Bacterial; D000818:Animals; D003094:Collagen / Q000378:metabolism; D003546:Cysteine Endopeptidases / Q000235:genetics / Q000502:physiology*; D010861:Fimbriae, Bacterial; D005816:Genetic Complementation Test; D000080867:Gingipain Cysteine Endopeptidases; D006388:Hemagglutinins / Q000235:genetics / Q000502:physiology*; D009154:Mutation; D016966:Porphyromonas gingivalis / Q000201:enzymology / Q000235:genetics / Q000472:pathogenicity*; D051381:Rats; D014774:Virulence
chemical_list: D018829:Adhesins, Bacterial; D000080867:Gingipain Cysteine Endopeptidases; D006388:Hemagglutinins; D003094:Collagen; D003546:Cysteine Endopeptidases