title: Factor V Leiden in Greek thrombophilic patients: relationship with activated protein C resistance test and levels of thrombin-antithrombin complex and prothrombin fragment 1 + 2.
abstract: We studied 172 Greek patients (72 men aged 44.0 +/- 16.7 years and 100 women aged 46.5 +/- 14.1 years) with an unexplained thrombophilic tendency. One hundred and four apparently healthy persons (63 men aged 34.2 +/- 10.0 years and 41 women aged 37.1 +/- 13.3 years) were included as a control group. We performed the activated protein C resistance (APC-r) test using a clotting test (Chromogenix kit), detection of factor V Leiden using polymerase chain reaction (PCR)-restriction fragment length polymorphisms and measurement of thrombin-antithrombin complexes (TAT) and prothrombin fragment 1 + 2 (F1 + 2) levels with an immunoenzymatic assay. The normal range for the APC-r test (> 2.12) was determined from the controls. The factor V Leiden mutation was found in 31.9% of all the patients tested, in 28.1% of the unrelated patients with documented thrombophilic tendency of unknown origin and in 4.8% of the healthy controls. The APC-r test had a sensitivity of 0.42 and a specificity of 0.91 for the detection of factor V Leiden. Furthermore, we found no significant difference in levels of TAT and F1 + 2 between patients with and without the mutation and there was no correlation between aPC-r values and levels of TAT and F1 + 2.
authors.affiliation: Biology Department, Medical Faculty, Aristotle University of Thessaloniki, Greece.
authors.forename: A F
authors.initials: AF
authors.lastname: Lambropoulos
authors.forename: Z
authors.initials: Z
authors.lastname: Foka
authors.forename: M
authors.initials: M
authors.lastname: Makris
authors.forename: M
authors.initials: M
authors.lastname: Daly
authors.forename: A
authors.initials: A
authors.lastname: Kotsis
authors.forename: P E
authors.initials: PE
authors.lastname: Makris
mesh_terms: D000328:Adult; D000990:Antithrombin III / Q000378:metabolism*; D016022:Case-Control Studies; D005165:Factor V / Q000378:metabolism*; D005260:Female; D006115:Greece / Q000453:epidemiology; D006801:Humans; D008297:Male; D008875:Middle Aged; D009154:Mutation; D010446:Peptide Fragments / Q000378:metabolism*; D010447:Peptide Hydrolases / Q000378:metabolism*; D015995:Prevalence; D011486:Protein C / Q000502:physiology*; D011516:Prothrombin / Q000378:metabolism*; D019851:Thrombophilia / Q000097:blood* / Q000453:epidemiology
chemical_list: D010446:Peptide Fragments; D011486:Protein C; C046193:antithrombin III-protease complex; C095381:factor V Leiden; C045879:prothrombin fragment 1.2; D000990:Antithrombin III; D005165:Factor V; D011516:Prothrombin; D010447:Peptide Hydrolases