title: Single-strand conformation polymorphism (SSCP) analysis of Listeria monocytogenes iap gene as tool to detect different serogroups.
abstract: PCR-single-strand conformation polymorphism (PCR-SSCP) analysis is a convenient technique for the detection of mutations. As the mobility of single-stranded DNA is sequence-dependent it could therefore be used to determine serotype-related sequence variations in Listeria monocytogenes. Sero-specific patterns were observed in different L. monocytogenes serogroups.
authors.affiliation: Dipartimento di Scienze degli Alimenti, Facoltà di Agraria, Università di Udine, Italy.
authors.forename: M
authors.initials: M
authors.lastname: Manzano
authors.forename: L
authors.initials: L
authors.lastname: Cocolin
authors.forename: C
authors.initials: C
authors.lastname: Pipan
authors.forename: E
authors.initials: E
authors.lastname: Falasca
authors.forename: G A
authors.initials: GA
authors.lastname: Botta
authors.forename: C
authors.initials: C
authors.lastname: Cantoni
authors.forename: G
authors.initials: G
authors.lastname: Comi
mesh_terms: D001426:Bacterial Proteins / Q000235:genetics*; D017931:DNA Primers; D014644:Genetic Variation; D006801:Humans; D008089:Listeria monocytogenes / Q000145:classification* / Q000235:genetics*; D016133:Polymerase Chain Reaction / Q000379:methods; D018807:Polymorphism, Single-Stranded Conformational; D015203:Reproducibility of Results; D012703:Serotyping / Q000379:methods
chemical_list: C078516:60 kDa protein, Listeria monocytogenes; D001426:Bacterial Proteins; D017931:DNA Primers