Synergy between TGF-beta 3 and NT-3 to promote the survival of spiral ganglia neurones in vitro.
Transforming growth factor-betas (TGF-betas) have been implicated in normal inner ear development and in promoting neuronal survival. Early rat post-natal spiral ganglion cells (SGC) in dissociated cell culture were used as a model of auditory innervation to test the trophic factors TGF-beta3 and neurotrophin-3 (NT-3) for their ability, individually or in combination, to promote neuronal survival. The findings from this study suggest that TGF-beta3 supports neuronal survival in a concentration-dependent manner. Moreover TGF-beta3 and NT-3-potentiated spiral ganglion neuronal survival in a synergistic fashion.
Marzella
P L
PL
Department of Otolaryngology, Victorian Royal Eye and Ear Hospital, East Melbourne, Australia. marzellap@mail.medoto.unimelb.edu.au
Clark
G M
GM
Shepherd
R K
RK
Bartlett
P F
PF
Kilpatrick
T J
TJ
D000818:Animals; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D004305:Dose-Response Relationship, Drug; D004357:Drug Synergism; D009414:Nerve Growth Factors / Q000494:pharmacology*; D009474:Neurons / Q000166:cytology* / Q000187:drug effects; D020933:Neurotrophin 3; D051381:Rats; D017208:Rats, Wistar; D013136:Spiral Ganglion / Q000166:cytology* / Q000187:drug effects; D016212:Transforming Growth Factor beta / Q000494:pharmacology*
D009414:Nerve Growth Factors; D020933:Neurotrophin 3; D016212:Transforming Growth Factor beta