Practical approach for detection and identification of OXA-10-derived ceftazidime-hydrolyzing extended-spectrum beta-lactamases.
A practical approach to detect and identify ceftazidime-hydrolyzing extended-spectrum mutants of OXA-10 beta-lactamase is presented. Large numbers of bacteria were screened by colony hybridization, a 720-bp part of blaOXA was amplified by PCR from the hybridization-positive isolates, and the products were digested by PvuII and HaeIII.
Vahaboglu
H
H
Kocaeli Universitesi, Tip Fakültesi, Turkey. vahabo@uedh.com.tr
Ozturk
R
R
Akbal
H
H
Saribas
S
S
Tansel
O
O
Coşkunkan
F
F
D002442:Ceftazidime / Q000378:metabolism* / Q000494:pharmacology; D018550:Cephalosporin Resistance / Q000235:genetics; D002511:Cephalosporins / Q000378:metabolism* / Q000494:pharmacology; D006801:Humans; D009154:Mutation; D009693:Nucleic Acid Hybridization; D016133:Polymerase Chain Reaction; D011552:Pseudomonas Infections / Q000382:microbiology; D011550:Pseudomonas aeruginosa / Q000187:drug effects / Q000201:enzymology* / Q000235:genetics; D001618:beta-Lactamases / Q000032:analysis* / Q000235:genetics / Q000378:metabolism
D002511:Cephalosporins; D002442:Ceftazidime; C055187:beta-lactamase PSE-2; D001618:beta-Lactamases