Anti-alpha 4 integrin antibody induces apoptosis in murine thymocytes and staphylococcal enterotoxin B-activated lymph node T cells.
We have shown that an antibody (9C10) to the alpha 4 integrin induces apoptosis in murine immature CD4+ CD8+ thymocytes and in activated (but not resting) mature lymph node T cells. In both cases, apoptosis is blocked by the highly selective protein kinase C (PKC) inhibitor Ro31.8425, suggesting that 9C10 induces signalling through the alpha 4 integrin resulting in PKC activation leading to apoptosis. Overall, our results indicate the potential role of the alpha 4 integrin-mediated interactions in apoptosis induction during T-cell development and following mature T-cell activation.
Tchilian
E Z
EZ
Centre for Clinical Research in Immunology and Signalling/Department of Anatomy, Medical School, University of Birmingham, UK.
Owen
J J
JJ
Jenkinson
E J
EJ
D000818:Animals; D015703:Antigens, CD / Q000276:immunology*; D017209:Apoptosis / Q000187:drug effects / Q000276:immunology*; D004768:Enterotoxins / Q000276:immunology*; D005260:Female; D007211:Indoles / Q000494:pharmacology; D039441:Integrin alpha4; D008198:Lymph Nodes / Q000276:immunology; D008213:Lymphocyte Activation / Q000276:immunology; D008301:Maleimides / Q000494:pharmacology; D051379:Mice; D008807:Mice, Inbred BALB C; D011493:Protein Kinase C / Q000037:antagonists & inhibitors; D013211:Staphylococcus aureus / Q000276:immunology; D018089:Superantigens / Q000276:immunology*; D016176:T-Lymphocyte Subsets / Q000276:immunology*; D013950:Thymus Gland / Q000276:immunology
D015703:Antigens, CD; D004768:Enterotoxins; D007211:Indoles; D008301:Maleimides; D018089:Superantigens; C071924:Ro 31-8425; D039441:Integrin alpha4; C031818:enterotoxin B, staphylococcal; D011493:Protein Kinase C