Crystallization and preliminary crystallographic studies of a new crystal form of papain from Carica papaya.
A new crystal form of papain from the latex of Carica papaya, complexed with an inhibitor (Z-Arg-Leu-Val-Gly-CHN2) was obtained by the vapor-diffusion method using a methanol/ethanol mixture as a precipitant. The slat-like crystals are monoclinic, space group P2(1), with unit cell parameters a = 42.6 A, b = 49.8 A, c = 50.5 A, A = 111.9 degrees, and contain one molecule in the asymmetric unit. The crystals are stable in the X-ray beam and diffract beyond 1.8 A. A molecular model has been placed in the unit cell by molecular replacement.
Kozak
M
M
Department of Crystallography, Faculty of Chemistry, A. Mickiewicz University, Poznań, Poland.
Kozian
E
E
Grzonka
Z
Z
Jaskólski
M
M
D018360:Crystallography, X-Ray; D010206:Papain / Q000737:chemistry*; D010944:Plants / Q000737:chemistry*; D011487:Protein Conformation
D010206:Papain