title: TCRBV14S1 and rheumatoid arthritis revisited: abnormalities in the percentage of transcribed TCRBV14S1 family genes in PBMC from rheumatoid arthritis patients.
abstract: The purpose of this study was to identify Vbeta gene families that are associated with rheumatoid arthritis (RA). A PCR-based assay was used to compare the Vbeta repertoire of unstimulated PBMC from 18 RA patients and 18 matched controls. The influence of an HLA-DRB1-binding peptide (HA307-319) on the Vbeta repertoire of PBMC in culture was compared in 11 RA patients and 10 controls. There was a larger variance in the percentage of BV14S1 transcripts in unstimulated PBMC from RA patients than from controls (p = 0.0003). The mean percentage of BV14S1 transcripts was higher in RA patients when prednisone-treated RA patients were excluded from the analysis (p = 0.0006). In vitro stimulation with the HA307-319 peptide increased the percentage of BV14S1 transcripts in PBMC from RA patients (+ 1.5 +/- 0.4%, p < 0.005) but not controls (+ 0.3 +/- 0.2%, ns), and the difference between RA patients and controls was significant (p = 0.03). In conclusion, there is an association between RA and the BV14S1 gene family in New Zealand patients.
authors.affiliation: Department of Molecular Medicine, School of Medicine, Auckland, New Zealand.
authors.forename: W G
authors.initials: WG
authors.lastname: Abbott
authors.forename: P L
authors.initials: PL
authors.lastname: Tan
authors.forename: A
authors.initials: A
authors.lastname: Geursen
authors.forename: J D
authors.initials: JD
authors.lastname: Fraser
authors.forename: L
authors.initials: L
authors.lastname: Forbes
authors.forename: I J
authors.initials: IJ
authors.lastname: Simpson
authors.forename: S
authors.initials: S
authors.lastname: Yuan
authors.forename: M A
authors.initials: MA
authors.lastname: Skinner
mesh_terms: D001172:Arthritis, Rheumatoid / Q000188:drug therapy / Q000235:genetics* / Q000276:immunology; D002478:Cells, Cultured; D004247:DNA / Q000032:analysis; D005260:Female; D019674:Genes, T-Cell Receptor beta / Q000235:genetics*; D005938:Glucocorticoids / Q000627:therapeutic use; D006684:HLA-DR Antigens / Q000235:genetics; D059811:HLA-DRB1 Chains; D006801:Humans; D008213:Lymphocyte Activation; D008297:Male; D008875:Middle Aged; D016133:Polymerase Chain Reaction; D011241:Prednisone / Q000627:therapeutic use; D016693:Receptors, Antigen, T-Cell, alpha-beta / Q000235:genetics* / Q000276:immunology; D013601:T-Lymphocytes / Q000276:immunology; D014158:Transcription, Genetic
chemical_list: D005938:Glucocorticoids; D006684:HLA-DR Antigens; D059811:HLA-DRB1 Chains; D016693:Receptors, Antigen, T-Cell, alpha-beta; D004247:DNA; D011241:Prednisone