[Reorganization of elements of the cytoskeletal and vacuolar systems in tumor cells in the early stage of developing multiple drug resistance].
The multidrug resistance (MDR) is one of the main reasons for chemotherapeutic failures in cancer patients. The overexpression of MDR gene product (Pgp) leads to the appearance of resistant tumor cells. We investigated alterations of cell structures in cells with a low level MDR and Pgp expression. The Syrian hamster embryo fibroblasts transformed by the Raus sarcoma virus were used in our experiments. Resistant sublines were isolated by propagation of the cells in colchicine supplemented medium. We have found that the first steps of MDR are accompanied by significant alterations of cytoskeleton elements and vacuolar system, e.g. changes in actin stress-fibres organization, microtubule distribution, the Golgi complex structure, vesicular transport. We suppose that the above cell structural alterations are not accidental. Perhaps, they are also essential for evolution of multidrug resistant mechanisms.
Erokhina
M
M
Research Institute of Carcinogenesis, Moscow University.
Stavrovskaia
A
A
Onishchenko
G
G
D020168:ATP Binding Cassette Transporter, Subfamily B, Member 1 / Q000378:metabolism; D008841:Actin Cytoskeleton / Q000648:ultrastructure; D000818:Animals; D002461:Cell Line, Transformed; D006224:Cricetinae; D003599:Cytoskeleton / Q000648:ultrastructure*; D018432:Drug Resistance, Multiple; D019008:Drug Resistance, Neoplasm; D006056:Golgi Apparatus / Q000648:ultrastructure; D007382:Intermediate Filaments / Q000648:ultrastructure; D008647:Mesocricetus; D014617:Vacuoles / Q000648:ultrastructure*
D020168:ATP Binding Cassette Transporter, Subfamily B, Member 1
Reorganizatsiia élementov tsitoskeleta i vakuoliarnoĭ sistemy v opukholevykh kletkakh na rannikh étapakh razvitiia mnozhestvennoĭ lekarstvennoĭ ustoĭchvosti.