Effects of cadmium on haemopoiesis in irradiated and non-irradiated mice: 1. Relationship to the number of myeloid progenitor cells.
The effects of single subcutaneous injection of cadmium chloride on haemopoiesis in normal (non-irradiated) or irradiated mice were investigated. Cadmium doses used ranged from 1-8 mg/kg body weight Twenty-four hours after treatment with cadmium (doses from 3 to 8 mg/kg) there were no significant changes in bone marrow cellularity and the granulocyte-macrophage progenitor cell (GM-CFC) number per femur in non-irradiated female ICR mice. Similarly, during the 30-day postinjection period bone marrow cellularity and marrow GM-CFC number in mice treated with a cadmium dose of 5 mg/kg were not significantly different from the control values. Cadmium significantly reduced the lethal effects of gamma rays. In addition, increasing the doses of cadmium administered 24 h prior to sublethal irradiation increased the number of endogenous haemopoietic stem cells (endoCFU-S) in a concentration-dependent manner. Pretreatment with cadmium also decreased the radiation damage to endoCFU-S and haemopoietic progenitor cells committed to granulocyte/macrophage development (GM-CFC). The survival of stem cells was higher and the regeneration of cellularity and GM-CFC of irradiated bone marrow was accelerated in mice pretreated with 5 mg Cd/kg body weight in comparison with saline-injected mice.
Fedorocko
P
P
Department of Cellular and Molecular Biology, Faculty of Sciences, Safárik University, Moyzesova, Kosice, Slovak Republic.
Domonkosová
A
A
Kundrátová
T
T
Macková
N O
NO
Brezáni
P
P
Fedorocková
A
A
D000818:Animals; D001854:Bone Marrow Cells / Q000187:drug effects / Q000528:radiation effects; D002104:Cadmium / Q000494:pharmacology*; D005260:Female; D005720:Gamma Rays; D006098:Granulocytes / Q000187:drug effects / Q000528:radiation effects; D006410:Hematopoiesis / Q000187:drug effects* / Q000528:radiation effects*; D006412:Hematopoietic Stem Cells / Q000187:drug effects* / Q000528:radiation effects*; D008264:Macrophages / Q000187:drug effects / Q000528:radiation effects; D051379:Mice; D008813:Mice, Inbred ICR; D013154:Spleen / Q000166:cytology / Q000187:drug effects / Q000528:radiation effects; D013234:Stem Cells / Q000187:drug effects*; D016019:Survival Analysis
D002104:Cadmium