title: Establishment of a transgenic tobacco cell suspension culture system for producing murine granulocyte-macrophage colony stimulating factor.
abstract: We tested if murine granulocyte-macrophage colony stimulating factor (mGM-CSF) is produced as a biologically active form through plant cell culture. The mGM-CSF gene was cloned into the plant expression vector, pBI121, and Ti-plasmid mediated transformation of tobacco leaves was conducted using Agrobacterium tumefaciens harboring the recombinant mGM-CSF (rmGM-CSF) gene. Cell suspension culture was established from the leaf-derived calli of transgenic tobacco plants. Northern blot analysis indicated the expression of the introduced mGM-CSF gene in both transgenic plant and cell suspension cultures. In addition, the biological activity of rmGM-CSF from plant cell culture was confirmed by measuring the proliferation of GM-CSF dependent FDC-P1 cells.
authors.affiliation: Faculty of Biological Sciences, Chonbuk National University, Chonju, Korea.
authors.forename: J S
authors.initials: JS
authors.lastname: Lee
authors.forename: S J
authors.initials: SJ
authors.lastname: Choi
authors.forename: H S
authors.initials: HS
authors.lastname: Kang
authors.forename: W G
authors.initials: WG
authors.lastname: Oh
authors.forename: K H
authors.initials: KH
authors.lastname: Cho
authors.forename: T H
authors.initials: TH
authors.lastname: Kwon
authors.forename: D H
authors.initials: DH
authors.lastname: Kim
authors.forename: Y S
authors.initials: YS
authors.lastname: Jang
authors.forename: M S
authors.initials: MS
authors.lastname: Yang
mesh_terms: D016960:Agrobacterium tumefaciens / Q000235:genetics; D000818:Animals; D018929:Cell Culture Techniques / Q000379:methods*; D002455:Cell Division / Q000235:genetics; D015870:Gene Expression / Q000235:genetics; D005822:Genetic Vectors; D016178:Granulocyte-Macrophage Colony-Stimulating Factor / Q000235:genetics* / Q000502:physiology; D051379:Mice; D030821:Plants, Genetically Modified; D010947:Plants, Toxic; D012333:RNA, Messenger / Q000032:analysis; D011994:Recombinant Proteins / Q000235:genetics; D014026:Nicotiana / Q000235:genetics*; D014170:Transformation, Genetic / Q000235:genetics
chemical_list: D012333:RNA, Messenger; D011994:Recombinant Proteins; D016178:Granulocyte-Macrophage Colony-Stimulating Factor