An improved physical and genetic map of Campylobacter jejuni NCTC 11168 (UA580).
Campylobacter jejuni is recognized as the major cause of food-borne gastrointestinal disease in the developed world. To facilitate the molecular genetic analysis of this pathogen, an approximately 18-fold redundant Tropist3 cosmid library was constructed from C. jejuni NCTC 11168 genomic DNA. The cosmid library was partially ordered by hybridization to 15 pulsed-field electrophoresis (PFGE) restriction fragments. This analysis confirmed the estimated size of the genome to be 1730 kb, but suggested discrepancies in some regions of the published physical map. The precise locations of two of the three rRNA gene clusters were mapped using a combination of restriction fingerprinting, sample sequencing and riboprobing. Additionally, 15 further genes were located on the revised map. A more detailed physical and genetic map of C. jejuni NCTC 11168 is presented.
Karlyshev
Andrey V
AV
Department of Medical Microbiology, St Bartholomew's and the Royal London School of Medicine and Dentistry, West Smithfield, London EC1A 7BE, UK.
Henderson
John
J
Department of Genetics, University of Leicester, Leicester LE1 7RH, UK.
Ketley
Julian M
JM
Department of Genetics, University of Leicester, Leicester LE1 7RH, UK.
Wren
Brendan W
BW
Department of Medical Microbiology, St Bartholomew's and the Royal London School of Medicine and Dentistry, West Smithfield, London EC1A 7BE, UK.
D016123:Campylobacter jejuni / Q000235:genetics*; D002874:Chromosome Mapping; D003001:Cloning, Molecular; D004269:DNA, Bacterial / Q000032:analysis / Q000235:genetics; D015252:Deoxyribonucleases, Type II Site-Specific / Q000235:genetics / Q000378:metabolism; D016521:Electrophoresis, Gel, Pulsed-Field; D015723:Gene Library; D005798:Genes, Bacterial; D016680:Genome, Bacterial; D005810:Multigene Family; D009693:Nucleic Acid Hybridization; D010957:Plasmids; D012150:Polymorphism, Restriction Fragment Length; D015347:RNA Probes / Q000235:genetics; D012335:RNA, Ribosomal / Q000235:genetics; D011995:Recombination, Genetic
D004269:DNA, Bacterial; D015347:RNA Probes; D012335:RNA, Ribosomal; C025651:CCCGGG-specific type II deoxyribonucleases; D015252:Deoxyribonucleases, Type II Site-Specific; C029792:GTCGAC-specific type II deoxyribonucleases