title: [Amino acid sequence of the protein in a pure proteinic calculus].
abstract: OBJECTIVE
  The fact that for an equivalent oversaturation of solutes, urine of lithiasic patients has a greater tendency to form crystals, indicate that urine of stone-formers may contain substances that promote crystallization.
  
  
  METHODS
  A renal calculus was analyzed by infra-red spectrophotometry, x-ray dispersion energy spectrophotometry, ultraviolet absorption spectrophotometry, acid hydrolysis cationic exchange chromatography, SDA-Page gel electropheresis with Coomassie Blue and amino acid sequence.
  
  
  RESULTS
  It was a pure protein calculus formed by a beta 2-microglobulin anomaly. It was a residue that had lost its first 19 amino acids and phenylalanine replaced serine in position 19
  
  
  CONCLUSIONS
  Changes in the amino acid sequence of urinary proteins can cause these to behave like urinary calculus-promoting substances.
authors.affiliation: Hospital Universitario de Getafe, Madrid, España.
authors.forename: I
authors.initials: I
authors.lastname: Fernández González
authors.forename: F
authors.initials: F
authors.lastname: Lovaco Castellano
authors.forename: G
authors.initials: G
authors.lastname: Giménez Gallego
authors.forename: E
authors.initials: E
authors.lastname: García Cuerpo
mesh_terms: D000328:Adult; D000595:Amino Acid Sequence; D006801:Humans; D007669:Kidney Calculi / Q000737:chemistry*; D008297:Male; D008969:Molecular Sequence Data; D013055:Spectrophotometry, Infrared; D013056:Spectrophotometry, Ultraviolet; D001613:beta 2-Microglobulin / Q000737:chemistry*
chemical_list: D001613:beta 2-Microglobulin
vernacular_title: Secuencia de aminoácidos de la proteína de un cálculo proteico puro.