Evidence for co-existence of CCK-8 and GnRH in neurons of the mesencephalic tegmentum in the chameleon.
A double-label immunofluorescence technique was used to demonstrate the co-localization of cholecystokinin-8 (CCK-8) and gonadotrophin-releasing hormone (GnRH) in individual neurons and processes of the chameleon brain. Co-localization was limited to a small population of cells in the dorsomedial tegmentum; in other regions of the brain, neurons were observed to be either CCK-8-immunopositive or GnRH-immunopositive but never both. However, double-labeled fibers and terminals were found to be distributed at a low density throughout the thalamus, the medial hypothalamus, the tegmentum and the spinal cord. These data provide the first indication for the co-localization of CCK-8 and GnRH, whose functional significance remains to be established. ON
Bennis
M
M
Unité de Neurosciences du Comportement, Département de Biologie, Faculté des Sciences Semlalia, Marrakech, Morocco.
Repérant
J
J
Tramu
G
G
D000818:Animals; D005455:Fluorescent Antibody Technique; D007987:Gonadotropin-Releasing Hormone / Q000032:analysis* / Q000276:immunology; D007031:Hypothalamus / Q000737:chemistry / Q000166:cytology; D007150:Immunohistochemistry; D008116:Lizards; D009474:Neurons / Q000737:chemistry* / Q000378:metabolism; D012844:Sincalide / Q000032:analysis* / Q000276:immunology; D013681:Tegmentum Mesencephali / Q000737:chemistry* / Q000166:cytology; D013788:Thalamus / Q000737:chemistry / Q000166:cytology
D007987:Gonadotropin-Releasing Hormone; D012844:Sincalide