title: Expression of corticotrophin-releasing hormone receptor mRNA and protein in the human myometrium.
abstract: The reported effects of corticotrophin-releasing hormone (CRH) on human myometrium support the existence of specific receptors for the hormone in this tissue. We have used the reverse transcriptase-polymerase chain reaction (RT-PCR) technique to study the expression of mRNA coding for the CRH R1 and R2 receptors. RT-PCR of total RNA from both nonpregnant and pregnant myometrium using specific primers resulted in amplification products of the expected sizes for the R1 alpha and R2 alpha CRH receptors. The identity of these amplification products was confirmed by specific restriction digests and sequencing. Immunohistochemistry using a rabbit antibody raised against a specific domain of the CRH R1 receptor demonstrated that the R1 mRNA is translated into protein and confirmed that it is the uterine smooth muscle cells from both nonpregnant and pregnant women that bear this receptor. Our results suggest that CRH may play a role in human pregnancy at the myometrium.
authors.affiliation: Nuffield Department of Obstetrics and Gynaecology, University of Oxford, John Radcliffe Hospital, UK.
authors.forename: B
authors.initials: B
authors.lastname: Rodríguez-Liñares
authors.forename: E A
authors.initials: EA
authors.lastname: Linton
authors.forename: S
authors.initials: S
authors.lastname: Phaneuf
mesh_terms: D000328:Adult; D005260:Female; D015870:Gene Expression; D006801:Humans; D007150:Immunohistochemistry; D009215:Myometrium / Q000737:chemistry* / Q000166:cytology / Q000378:metabolism; D016133:Polymerase Chain Reaction; D011247:Pregnancy / Q000378:metabolism*; D012333:RNA, Messenger / Q000032:analysis*; D018019:Receptors, Corticotropin-Releasing Hormone / Q000032:analysis / Q000235:genetics / Q000378:metabolism*
chemical_list: D012333:RNA, Messenger; D018019:Receptors, Corticotropin-Releasing Hormone