title: Measles virus DNA vaccination: antibody isotype is determined by the method of immunization and by the nature of both the antigen and the coimmunized antigen.
abstract: Plasmids encoding the measles virus hemagglutinin (HA) and nucleoprotein (NP) proteins inoculated into the skin of BALB/c mice by the gene gun method induced both humoral and cytotoxic lymphocyte class I-restricted immune responses. Although intramuscular immunization induces the immunoglobulin G2a (IgG2a) antibody isotype for both antigens, with gene gun immunization, the NP still generated mainly IgG2a and the major isotype induced by the HA was IgG1. Interestingly, gene gun coimmunization of HA and NP plasmids resulted in a dominant IgG1 HA response and the switching of antibodies generated against the NP to the IgG1 isotype.
authors.affiliation: INSERM Unit 404 Immunity and Vaccination, Institut Pasteur de Lyon, France.
authors.forename: A I
authors.initials: AI
authors.lastname: Cardoso
authors.forename: N
authors.initials: N
authors.lastname: Sixt
authors.forename: A
authors.initials: A
authors.lastname: Vallier
authors.forename: J
authors.initials: J
authors.lastname: Fayolle
authors.forename: R
authors.initials: R
authors.lastname: Buckland
authors.forename: T F
authors.initials: TF
authors.lastname: Wild
mesh_terms: D000818:Animals; D000956:Antigens, Viral / Q000276:immunology*; D004279:DNA, Viral; D005260:Female; D006389:Hemagglutinins, Viral / Q000235:genetics / Q000276:immunology*; D007074:Immunoglobulin G / Q000276:immunology*; D007132:Immunoglobulin Isotypes / Q000276:immunology; D008458:Measles Vaccine / Q000276:immunology*; D008459:Measles virus / Q000276:immunology*; D051379:Mice; D008807:Mice, Inbred BALB C; D008809:Mice, Inbred C3H; D008810:Mice, Inbred C57BL; D008811:Mice, Inbred DBA; D019590:Nucleocapsid Proteins; D009698:Nucleoproteins / Q000235:genetics / Q000276:immunology*; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology; D014611:Vaccination; D014614:Vaccines, Synthetic / Q000276:immunology*; D014764:Viral Proteins / Q000235:genetics / Q000276:immunology*
chemical_list: D000956:Antigens, Viral; D004279:DNA, Viral; D006389:Hemagglutinins, Viral; D007074:Immunoglobulin G; D007132:Immunoglobulin Isotypes; D008458:Measles Vaccine; D019590:Nucleocapsid Proteins; D009698:Nucleoproteins; D014614:Vaccines, Synthetic; D014764:Viral Proteins; C032397:hemagglutinin protein G, measles virus; C058314:nucleoprotein, Measles virus