Indirect haemagglutination test using monoclonal antibody-affinity purified antigens for diagnosis of human paragonimiasis due to Paragonimus heterotremus.
An indirect haemagglutination test (IHA) using antigens purified by monoclonal antibody-affinity chromatography was developed for the diagnosis of human paragonimiasis caused by Paragonimus heterotremus. Sera from patients with paragonimiasis (n = 30) were evaluated, along with sera from other parasitic infections (n = 92), pulmonary tuberculosis (n = 18) and healthy controls (n = 30). The sensitivity, specificity as well as positive and negative predictive value of the IHA, calculated at the prevalence of disease at 17.6%, were all 100%.
Maleewong
W
W
Department of Parasitology, Faculty of Medicine, Khon Kaen University, Thailand.
Intapan
P M
PM
Wongkham
C
C
Pajongthanasaris
M P
MP
Morakote
N
N
Chaicumpa
W
W
D000818:Animals; D000909:Antibodies, Helminth / Q000097:blood*; D000911:Antibodies, Monoclonal / Q000276:immunology / Q000302:isolation & purification; D000947:Antigens, Helminth / Q000276:immunology*; D002846:Chromatography, Affinity; D006373:Helminthiasis / Q000175:diagnosis / Q000276:immunology; D006386:Hemagglutination Tests / Q000379:methods*; D006801:Humans; D007106:Immune Sera / Q000276:immunology; D010237:Paragonimiasis / Q000175:diagnosis* / Q000276:immunology / Q000469:parasitology; D010238:Paragonimus / Q000276:immunology*; D012680:Sensitivity and Specificity; D014376:Tuberculosis / Q000175:diagnosis / Q000276:immunology
D000909:Antibodies, Helminth; D000911:Antibodies, Monoclonal; D000947:Antigens, Helminth; D007106:Immune Sera