title: Calcium mobilization evoked by amyloid beta-protein involves inositol 1,4,5-triphosphate production in human platelets.
abstract: We examined the effects of amyloid beta-protein (A beta) on Ca2+ mobilization in human platelets. The addition of A beta fragments 25-35 (A beta 25-35) gradually increased the cytoplasmic free Ca2+ concentration ([Ca2+]i). After the maximum response, [Ca2+]i decreased and then reached a sustained, higher level of [Ca2+]i. Similar effects were also observed with A beta 1-40, whereas 1-28 , 12-28 and 31-35 did not affect the Ca2+ response. In the absence of extracellular Ca2+, A beta 25-35 caused a transient increase in [Ca2+]i, which returned to the resting level. U73122, a phospholipase C inhibitor, completely abolished Ca2+ mobilization induced by thrombin and A beta 25-35. Furthermore, A beta enhanced the production of inositol 1,4,5-trisphosphate (IP3) in platelets. These findings suggest that Ca2+ mobilization induced by A beta 25-35 is due to phospholipase C activation and IP3 production.
authors.affiliation: Department of Neuropsychiatry, School of Medicine, Sapporo Medical University, Japan.
authors.forename: H
authors.initials: H
authors.lastname: Ishikawa
authors.forename: H
authors.initials: H
authors.lastname: Ozawa
authors.forename: T
authors.initials: T
authors.lastname: Saito
authors.forename: N
authors.initials: N
authors.lastname: Takahata
authors.forename: H
authors.initials: H
authors.lastname: Takemura
mesh_terms: D016229:Amyloid beta-Peptides / Q000494:pharmacology*; D001792:Blood Platelets / Q000187:drug effects* / Q000378:metabolism; D002118:Calcium / Q000378:metabolism*; D006801:Humans; D015544:Inositol 1,4,5-Trisphosphate / Q000096:biosynthesis*
chemical_list: D016229:Amyloid beta-Peptides; D015544:Inositol 1,4,5-Trisphosphate; D002118:Calcium