Effects of VIP and related peptides on airway mucus secretion from isolated rat trachea.
Vasoactive intestinal polypeptide (VIP) is known as an important regulator of airway function. It has been suggested that VIP is involved in the pathogenesis of asthma due to its relaxant effects on smooth muscles. The present study was designed to characterize the effects of the peptides of the VIP family on airway mucus secretion. The peptides VIP, PHI, PACAP-27, PACAP-38, GLP-I, exendin-4, helodermin, helospectin I and helospectin II were investigated using isolated rat trachea. Data show that PACAP-27 is the most potent stimulator of airway mucus secretion (225% stimulation). The rank order of potency was PACAP-27 > VIP > helospectin II > PHI > exendin-4 = helodermin = helospectin I = PACAP-38. The addition of the protease inhibitor thiorphan enhanced the effects of PHI and helodermin, but not of the other peptides. These data show that the peptides of the VIP family stimulate airway mucus secretion differently.
Wagner
U
U
Department of Medicine, Philipps-University of Marburg, Germany.
Bredenbröker
D
D
Storm
B
B
Tackenberg
B
B
Fehmann
H C
HC
von Wichert
P
P
D000818:Animals; D000077270:Exenatide; D005934:Glucagon / Q000494:pharmacology; D052216:Glucagon-Like Peptide 1; D066298:In Vitro Techniques; D036341:Intercellular Signaling Peptides and Proteins; D008297:Male; D009093:Mucus / Q000378:metabolism*; D009479:Neuropeptides / Q000494:pharmacology; D010446:Peptide Fragments / Q000494:pharmacology; D010451:Peptide PHI / Q000494:pharmacology; D010455:Peptides / Q000494:pharmacology / Q000502:physiology; D051219:Pituitary Adenylate Cyclase-Activating Polypeptide; D011480:Protease Inhibitors / Q000494:pharmacology; D011498:Protein Precursors / Q000494:pharmacology; D051381:Rats; D017207:Rats, Sprague-Dawley; D015244:Thiorphan / Q000494:pharmacology; D014132:Trachea / Q000187:drug effects* / Q000378:metabolism*; D014660:Vasoactive Intestinal Peptide / Q000494:pharmacology*; D014688:Venoms
C495403:Adcyap1 protein, rat; D036341:Intercellular Signaling Peptides and Proteins; D009479:Neuropeptides; D010446:Peptide Fragments; D010451:Peptide PHI; D010455:Peptides; D051219:Pituitary Adenylate Cyclase-Activating Polypeptide; D011480:Protease Inhibitors; D011498:Protein Precursors; D014688:Venoms; D014660:Vasoactive Intestinal Peptide; C040442:heliodermin; D052216:Glucagon-Like Peptide 1; D005934:Glucagon; C043046:helospectin I; C043047:helospectin II; D000077270:Exenatide; D015244:Thiorphan