Single-strand conformation polymorphism (SSCP) analysis of Listeria monocytogenes iap gene as tool to detect different serogroups.
PCR-single-strand conformation polymorphism (PCR-SSCP) analysis is a convenient technique for the detection of mutations. As the mobility of single-stranded DNA is sequence-dependent it could therefore be used to determine serotype-related sequence variations in Listeria monocytogenes. Sero-specific patterns were observed in different L. monocytogenes serogroups.
Manzano
M
M
Dipartimento di Scienze degli Alimenti, Facoltà di Agraria, Università di Udine, Italy.
Cocolin
L
L
Pipan
C
C
Falasca
E
E
Botta
G A
GA
Cantoni
C
C
Comi
G
G
D001426:Bacterial Proteins / Q000235:genetics*; D017931:DNA Primers; D014644:Genetic Variation; D006801:Humans; D008089:Listeria monocytogenes / Q000145:classification* / Q000235:genetics*; D016133:Polymerase Chain Reaction / Q000379:methods; D018807:Polymorphism, Single-Stranded Conformational; D015203:Reproducibility of Results; D012703:Serotyping / Q000379:methods
C078516:60 kDa protein, Listeria monocytogenes; D001426:Bacterial Proteins; D017931:DNA Primers