title: Polyamine depletion protects HL-60 cells from 2-deoxy-D-ribose-induced apoptosis.
abstract: We investigated the involvement of natural polyamines in HL-60 cell death triggered by exposure to 2-deoxy-D-ribose (dRib). In contrast to previous studies, exogenous polyamines failed to protect HL-60 cells against apoptosis caused by dRib. Moreover, in our experimental conditions, depletion of intracellular levels of putrescine and spermidine by alpha-difluoromethylornithine (DFMO) delayed the onset of apoptosis by at least a day or so. Exogenous polyamines reversed the beneficial effect of DFMO and restored the apoptotic levels observed in dRib-treated cells. We suggested that polyamines, especially putrescine and spermidine, act as facilitating factors in the induction of apoptosis triggered by dRib in HL-60 cells.
authors.forename: M G
authors.initials: MG
authors.lastname: Monti
authors.forename: S
authors.initials: S
authors.lastname: Ghiaroni
authors.forename: L
authors.initials: L
authors.lastname: Pernecco
authors.forename: D
authors.initials: D
authors.lastname: Barbieri
authors.forename: G
authors.initials: G
authors.lastname: Marverti
authors.forename: C
authors.initials: C
authors.lastname: Franceschi
mesh_terms: D017209:Apoptosis / Q000187:drug effects*; D053938:DNA Fragmentation; D003855:Deoxyribose / Q000494:pharmacology*; D000518:Eflornithine / Q000494:pharmacology; D004791:Enzyme Inhibitors / Q000494:pharmacology; D018922:HL-60 Cells; D006801:Humans; D065108:Ornithine Decarboxylase Inhibitors; D011700:Putrescine / Q000378:metabolism*; D013095:Spermidine / Q000378:metabolism*
chemical_list: D004791:Enzyme Inhibitors; D065108:Ornithine Decarboxylase Inhibitors; D003855:Deoxyribose; D013095:Spermidine; D011700:Putrescine; D000518:Eflornithine