title: A simple hardware model for the direct observation of voltage-clamp performance under realistic conditions.
abstract: A new hardware cell model for electrophysiological recording has been constructed which allows for the assessment of voltage clamp accuracy in different recording situations. Each compartment consists of a capacitor in parallel with a variable resistor and can be connected to other compartments by a variable axial resistance. The simulated membrane resistance can be changed extrinsically by a command voltage input which is optically coupled to the cell without any direct galvanic contact. Each compartment possesses a buffer amplifier which reads out the potential at the simulated membrane element, (e.g. 'somatic' or 'dendritic' potential). The model allows for the direct observation of typical situations and problems arising in electrophysiological experiments. We used the model to monitor deviations between the 'intracellular' and the command voltage, e.g. due to series resistance errors. We also used the model to simulate synaptic currents which were generated by triangular membrane conductance changes. The results demonstrate the strong influence of synaptic location and series resistance on voltage clamp fidelity. The cell model is a new and easy-to-handle tool for the observation of voltage control under realistic experimental conditions.
authors.affiliation: Institut für Physiologie der Charité, Berlin, Germany. draguhn@rz.charite.hu-berlin.de
authors.forename: A
authors.initials: A
authors.lastname: Draguhn
authors.forename: M
authors.initials: M
authors.lastname: Pfeiffer
authors.forename: U
authors.initials: U
authors.lastname: Heinemann
authors.forename: R
authors.initials: R
authors.lastname: Polder
mesh_terms: D000818:Animals; D002138:Calibration; D002462:Cell Membrane / Q000502:physiology; D003712:Dendrites / Q000502:physiology*; D004553:Electric Conductivity; D008564:Membrane Potentials; D008838:Microcomputers; D008959:Models, Neurological; D018408:Patch-Clamp Techniques / Q000295:instrumentation; D013569:Synapses / Q000502:physiology*