title: Cutaneous leishmaniasis: iso-enzyme characterisation of Leishmania tropica.
abstract: In order to identify and characterise the organisms responsible for Cutaneous Leishmaniasis, parasites were isolated from active lesions, grown in-vitro cultures and identified by iso-enzyme characterisation. Thirteen isolates from different patients were typed as L. tropica. Seven of these isolates were from Afghan refugees encamped in the suburbs of Islamabad, 3 were from patients in Multan, 1 was from a patient from Azad Jammu and Kashmir and 1 was from Besham (Swat, NWFP). The study confirms the presence of anthroponotic Cutaneous Leishamaniasis caused by L. Tropica in Pakistan.
authors.affiliation: Health Services Academy, Ministry of Health and National Institute of Health, Islamabad.
authors.forename: M A
authors.initials: MA
authors.lastname: Rab
authors.forename: L
authors.initials: L
authors.lastname: al Rustamani
authors.forename: R A
authors.initials: RA
authors.lastname: Bhutta
authors.forename: M T
authors.initials: MT
authors.lastname: Mahmood
authors.forename: D A
authors.initials: DA
authors.lastname: Evans
mesh_terms: D000293:Adolescent; D000328:Adult; D000346:Afghanistan / Q000208:ethnology; D000410:Alanine Transaminase / Q000032:analysis; D000626:Aminopeptidases / Q000032:analysis; D000818:Animals; D001219:Aspartate Aminotransferases / Q000032:analysis; D002648:Child; D002675:Child, Preschool; D004592:Electrophoresis, Starch Gel; D004950:Esterases / Q000032:analysis; D005260:Female; D005956:Glucose-6-Phosphate Isomerase / Q000032:analysis; D006801:Humans; D007527:Isoenzymes / Q000032:analysis*; D007895:Leishmania tropica / Q000145:classification* / Q000201:enzymology; D016773:Leishmaniasis, Cutaneous / Q000469:parasitology*; D008291:Malate Dehydrogenase / Q000032:analysis; D008297:Male; D008359:Mannose-6-Phosphate Isomerase / Q000032:analysis; D008828:Microbiological Techniques; D009699:N-Glycosyl Hydrolases / Q000032:analysis; D010154:Pakistan; D010733:Phosphoglucomutase / Q000032:analysis; D010734:Phosphogluconate Dehydrogenase / Q000032:analysis; D011770:Pyruvate Kinase / Q000032:analysis; D012036:Refugees; D013482:Superoxide Dismutase / Q000032:analysis
chemical_list: D007527:Isoenzymes; D008291:Malate Dehydrogenase; D010734:Phosphogluconate Dehydrogenase; D013482:Superoxide Dismutase; D001219:Aspartate Aminotransferases; D000410:Alanine Transaminase; D011770:Pyruvate Kinase; D004950:Esterases; D009699:N-Glycosyl Hydrolases; C030681:purine nucleosidase; D000626:Aminopeptidases; C022450:prolyl aminopeptidase; D008359:Mannose-6-Phosphate Isomerase; D005956:Glucose-6-Phosphate Isomerase; D010733:Phosphoglucomutase