In vivo model to study the biocompatibility of peritoneal dialysis solutions.
This study was designed to analyze the complex morphologic and functional effects of dialysis solutions on peritoneum in a rat model on chronic peritoneal dialysis. Peritoneal catheters were inserted into 10 male, Wistar rats and the animals were dialyzed twice daily for 4 weeks with 4.25% Dianeal. During the study we observed two opposite effects: healing of the peritoneum after catheter implantation--decreased cell count in dialysate, decreased permeability of the peritoneum to glucose and total protein, increased volume of drained dialysate; and damage to the membrane due to its exposure to peritoneal dialysis solution--increased hyaluronic acid levels in dialysate, a tendency of the peritoneum to thicken when compared to non-dialyzed animals. Our rat model of CAPD may be used for quantitative and qualitative assessment of the effects of peritoneal dialysis solution on the peritoneum during chronic dialysis.
Wieczorowska-Tobis
K
K
Department of Pathophysiology, University Medical School, Poznan, Poland.
Korybalska
K
K
Polubinska
A
A
Radkowski
M
M
Breborowicz
A
A
Oreopoulos
D G
DG
D000818:Animals; D002404:Catheterization; D015314:Dialysis Solutions / Q000737:chemistry / Q000494:pharmacology*; D005947:Glucose / Q000032:analysis / Q000378:metabolism; D006820:Hyaluronic Acid / Q000032:analysis / Q000378:metabolism; D007958:Leukocyte Count; D008297:Male; D010531:Peritoneal Dialysis, Continuous Ambulatory; D010537:Peritoneum / Q000187:drug effects* / Q000473:pathology; D011506:Proteins / Q000032:analysis / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D014508:Urea / Q000032:analysis / Q000378:metabolism; D014945:Wound Healing
D015314:Dialysis Solutions; D011506:Proteins; D014508:Urea; D006820:Hyaluronic Acid; D005947:Glucose