Standardized BACTEC method to measure clarithromycin susceptibility of Mycobacterium genavense.
A standardized clarithromycin susceptibility test for Mycobacterium genavense is reported. The BACTEC radiometric broth dilution test method recommended for Mycobacterium avium complex was modified to develop a reliable and reproducible procedure. Test development involved optimization of medium pH and inoculum densities for antibiotic vials as well as growth control vials. MIC control organisms included Mycobacterium simiae, Mycobacterium avium, and Mycobacterium xenopi. Growth control vials required two to three inoculum dilutions, which varied for each species. Clarithromycin MICs and MBCs for 12 isolates and 1 colonial variant of M. genavense ranged from < or = 0.06 to 0.25 microg/ml.
Carlson
L D
LD
Department of Laboratory Medicine, Harborview Medical Center, University of Washington, Seattle 98104, USA.
Wallis
C K
CK
Coyle
M B
MB
D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D017291:Clarithromycin / Q000494:pharmacology*; D003470:Culture Media; D006801:Humans; D006863:Hydrogen-Ion Concentration; D008826:Microbial Sensitivity Tests / Q000379:methods* / Q000592:standards; D009161:Mycobacterium / Q000187:drug effects* / Q000254:growth & development; D009164:Mycobacterium Infections / Q000382:microbiology; D015203:Reproducibility of Results
D000900:Anti-Bacterial Agents; D003470:Culture Media; D017291:Clarithromycin