title: Expression of apo-aequorin during embryonic development; how much is needed for calcium imaging?
abstract: Aequorin is a bioluminescent calcium indicator consisting of a 21 kDa protein (apo-aequorin) that is covalently linked to a lipophilic cofactor (coelenterazine). The aequorin gene can be expressed in a variety of cell lines and tissues, allowing non-invasive calcium imaging of specific cell types. In the present paper, we describe the possibilities and limitations of calcium imaging with genetically introduced apo-aequorin during embryonic development. By injecting aequorin into sea urchin, Drosophila and zebrafish eggs, we found that higher aequorin concentrations are needed in smaller eggs. Our results suggest that for measuring resting levels of free cytosolic calcium, one needs aequorin concentrations of at least 40 microM in sea urchin eggs, 2 microM in Drosophila eggs, and only 0.11 microM in zebrafish eggs. A simple assay was used to determine the absolute concentrations of expressed apo-aequorin and the percentage of aequorin formation in vivo. The use of this assay is illustrated by expression of the aequorin gene in Drosophila oocytes. These oocytes form up to 1 microM apo-aequorin. In our hands, only 0.3% of this apo-aequorin combined with coelenterazine entering from the medium to form aequorin, which was not enough for calcium imaging of the oocytes, but did allow in vivo imaging of the ovaries. From these studies, we conclude that coelenterazine entry into the cell is the rate limiting step in aequorin formation. Based on the rate of coelenterazine uptake in Drosophila, we estimate that complete conversion of 1 microM apo-aequorin would take 50 days in zebrafish eggs, 2 days [corrected] in Drosophila eggs, 7 days in sea urchin eggs or 18 h in a 10 microm tissue culture cell. Our results suggest that work based on genetically introduced apo-aequorin will be most successful when large amounts of small cells can be incubated in coelenterazine. During embryonic development this would involve introducing coelenterazine into the circulatory system of late stage embryos. Calcium imaging in early stage embryos may be best done by injecting aequorin, which circumvents the slow process of coelenterazine entry.
authors.affiliation: Marine Biological Laboratory, Woods Hole, Massachusetts 02543, USA. rcreton@mbl.edu
authors.forename: R
authors.initials: R
authors.lastname: Créton
authors.forename: M E
authors.initials: ME
authors.lastname: Steele
authors.forename: L F
authors.initials: LF
authors.lastname: Jaffe
mesh_terms: D000331:Aequorin / Q000096:biosynthesis* / Q000235:genetics / Q000378:metabolism; D000818:Animals; D030801:Animals, Genetically Modified; D001059:Apoproteins / Q000096:biosynthesis* / Q000235:genetics / Q000378:metabolism; D002118:Calcium / Q000032:analysis*; D002135:Calcium-Binding Proteins / Q000096:biosynthesis* / Q000235:genetics / Q000378:metabolism; D048429:Cell Size; D004330:Drosophila / Q000196:embryology; D047108:Embryonic Development; D007202:Indicators and Reagents; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000235:genetics / Q000378:metabolism; D012617:Sea Urchins / Q000196:embryology; D015027:Zebrafish / Q000196:embryology
chemical_list: D001059:Apoproteins; D002135:Calcium-Binding Proteins; D007202:Indicators and Reagents; D011994:Recombinant Proteins; C060014:apoaequorin; D000331:Aequorin; D002118:Calcium