Antiserum against neuropeptide FF augments vasopressin release in conscious rats.
We previously reported that centrally administered neuropeptide FF (NPFF) inhibited arginine vasopressin (AVP) release. In this study, immunoneutralization of central NPFF was performed to evaluate the role of endogenous NPFF in the regulation of AVP release. Intracerebroventricular (ICV) injection of antiserum against NPFF (Anti-NPFF) significantly augmented the plasma AVP increase induced by hyperosmolality [intraperitoneal injection of hypertonic saline (600 mOsm/kg, 2% BW)] at 60 min after ICV injection compared with normal rabbit serum (NRS) (NRS: 4.20+/-0.30 pg/ml, Anti-NPFF: 5.83+/-0.46 pg/ml, p < 0.01). Anti-NPFF did not cause significant change in plasma osmolality, plasma volume or arterial blood pressure. This evidence indicates that endogenous NPFF might be physiologically involved in osmoregulation of the plasma AVP level through its inhibitory action.
Yokoi
H
H
First Department of Internal Medicine, Nagoya University School of Medicine, Japan.
Arima
H
H
Kondo
K
K
Murase
T
T
Iwasaki
Y
Y
Yang
H Y
HY
Oiso
Y
Y
D000595:Amino Acid Sequence; D000818:Animals; D000906:Antibodies / Q000008:administration & dosage; D001127:Arginine Vasopressin / Q000097:blood / Q000378:metabolism*; D001798:Blood Proteins / Q000378:metabolism; D007276:Injections, Intraventricular; D008297:Male; D009479:Neuropeptides / Q000037:antagonists & inhibitors* / Q000276:immunology / Q000502:physiology; D009842:Oligopeptides / Q000037:antagonists & inhibitors* / Q000276:immunology / Q000502:physiology; D011817:Rabbits; D051381:Rats; D017207:Rats, Sprague-Dawley; D012964:Sodium / Q000097:blood; D014882:Water-Electrolyte Balance / Q000502:physiology
D000906:Antibodies; D001798:Blood Proteins; D009479:Neuropeptides; D009842:Oligopeptides; D001127:Arginine Vasopressin; C053651:phenylalanyl-leucyl-phenylalanyl-glutaminyl-prolyl-glutaminyl-arginyl-phenylalaninamide; D012964:Sodium