The pufferfish SLP-1 gene, a new member of the SCL/TAL-1 family of transcription factors.
The SCL/TAL-1 gene encodes a basic helix-loop-helix (bHLH) transcription factor essential for the development of all hemopoietic lineages and also acts as a T-cell oncogene. Four related genes have been described in mammals (LYL-1, TAL-2, NSCL1, and NSCL2), all of which exhibit a high degree of sequence similarity to SCL/TAL-1 in the bHLH domain and two of which (LYL-1 and TAL-2) have also been implicated in the pathogenesis of T-cell acute lymphoblastic leukemia. In this study we describe the identification and characterization of a pufferfish gene termed SLP-1, which represents a new member of this gene family. The genomic structure and sequence of SLP-1 suggests that it forms a subfamily with SCL/TAL-1 and LYL-1 and is most closely related to SCL/TAL-1. However, unlike SCL/TAL-1, SLP-1 is widely expressed. Sequence analysis of a whole cosmid containing SLP-1 shows that SLP-1 is flanked upstream by a zinc finger gene and a fork-head-domain gene and downstream by a heme-oxygenase and a RING finger gene.
Göttgens
B
B
Department of Haematology, MRC Centre, University of Cambridge, United Kingdom.
Gilbert
J G
JG
Barton
L M
LM
Aparicio
S
S
Hawker
K
K
Mistry
S
S
Vaudin
M
M
King
A
A
Bentley
D
D
Elgar
G
G
Green
A R
AR
D000595:Amino Acid Sequence; D000818:Animals; D015152:Blotting, Northern; D004268:DNA-Binding Proteins / Q000235:genetics*; D029941:Fish Proteins; D005400:Fishes, Poisonous / Q000235:genetics*; D015870:Gene Expression; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D011518:Proto-Oncogene Proteins; D017386:Sequence Homology, Amino Acid; D014157:Transcription Factors / Q000235:genetics*
D004268:DNA-Binding Proteins; D029941:Fish Proteins; D011518:Proto-Oncogene Proteins; C111555:SLP-1 protein, Fugu rubripes; D014157:Transcription Factors