An improved method for propagating oligodendrocyte progenitors in vitro.
Many investigators studying oligodendrocytes in vitro have sought out cell lines because it has been difficult to obtain sufficient numbers of primary oligodendrocytes for study. This paper describes three methodological improvements that facilitate culturing oligodendrocytes. We show that by detaching progenitor cells using papain instead of trypsin the total yield of oligodendrocyte progenitors can be doubled. We also show that papain can be used to subculture differentiated oligodendrocytes. Finally we report that primary O-2A progenitors can be cryo-preserved, reducing the demand upon laboratory personnel to produce and propagate them.
Young
G M
GM
Department of Neuroscience and Anatomy, College of Medicine, Pennsylvania State University, Hershey 17033, USA.
Levison
S W
SW
D000818:Animals; D002452:Cell Count; D018929:Cell Culture Techniques / Q000379:methods*; D002454:Cell Differentiation; D002469:Cell Separation / Q000379:methods; D002478:Cells, Cultured; D015925:Cryopreservation / Q000379:methods; D017077:Culture Media, Conditioned; D009836:Oligodendroglia / Q000166:cytology* / Q000378:metabolism; D010206:Papain; D051381:Rats; D013234:Stem Cells / Q000166:cytology* / Q000378:metabolism; D014357:Trypsin
D017077:Culture Media, Conditioned; D014357:Trypsin; D010206:Papain