Mycobactericidal activity of human natural, monoclonal, and recombinant yeast killer toxin-like antibodies.
Human natural (KTAb), murine monoclonal (KTMAb), and single-chain recombinant (KTScFv) candidacidal antibodies representing the internal image of a killer toxin from the yeast Pichia anomala (KT), characterized by a wide spectrum of antibiotic activity, exerted a lethal effect against a KT-sensitive multidrug-resistant isolate of Mycobacterium tuberculosis. KTMAb and KTScFv were produced by the hybridoma and DNA technologies, respectively, from the spleen lymphocytes of animals immunized with the idiotype of a KT-neutralizing MAb (MAb KT4), while KTAb were purified against MAb KT4 from the vaginal fluid of women infected with Candida albicans cells bearing an idiotype-like KT cell wall receptor. Mycobactericidal activity was related to the binding of KTAb, KTMAb, and KTScFv to the cell surface of KT-sensitive bacterial cells and was prevented by specific absorption of KT-like antibodies onto MAb KT4. These data identify a novel potentially useful immunotherapeutic approach to tuberculosis.
Conti
S
S
Istituto di Microbiologia, Facoltà di Medicina e Chirurgia, Università degli Studi di Parma, Italy.
Fanti
F
F
Magliani
W
W
Gerloni
M
M
Bertolotti
D
D
Salati
A
A
Cassone
A
A
Polonelli
L
L
D000888:Antibodies, Anti-Idiotypic / Q000494:pharmacology*; D000911:Antibodies, Monoclonal / Q000494:pharmacology; D000995:Antitubercular Agents / Q000494:pharmacology*; D004352:Drug Resistance, Microbial; D018432:Drug Resistance, Multiple; D055646:Killer Factors, Yeast; D009169:Mycobacterium tuberculosis / Q000187:drug effects*; D009183:Mycotoxins / Q000276:immunology*; D010843:Pichia / Q000276:immunology*; D011956:Receptors, Cell Surface; D011994:Recombinant Proteins / Q000494:pharmacology
D000888:Antibodies, Anti-Idiotypic; D000911:Antibodies, Monoclonal; D000995:Antitubercular Agents; D055646:Killer Factors, Yeast; D009183:Mycotoxins; D011956:Receptors, Cell Surface; D011994:Recombinant Proteins; C087906:killer toxin, Pichia