HSC-2DPAGE and the two-dimensional gel electrophoresis database of dog heart proteins.
A two-dimensional gel electrophoresis database of dog (Canis familiaris) proteins is presented. The database contains 1212 protein spots which have been characterised in terms of their pI and Mr. This database has been integrated into the HSC-2DPAGE database which is accessible on the Internet via the World Wide Web with the uniform resource location (URL): (http://www.harefield.nthames.nhs.uk/nhli/ protein/index.html). Identifications for 80 of the protein spots have been obtained by visual cross-matching with the human heart protein database in HSC-2DPAGE (42 spots), N-terminal microsequence analysis (25 spots) and peptide mass fingerprinting (20 spots). This database is being used in studies of alterations in protein expression in models of heart failure and heart disease.
Dunn
M J
MJ
Cardiothoracic Surgery, National Heart and Lung Institute, Imperial College School of Medicine, Harefield Hospital, UK. mike.dunn@harefield.nthames.nhs.uk
Corbett
J M
JM
Wheeler
C H
CH
D000595:Amino Acid Sequence; D000818:Animals; D003195:Computer Communication Networks; D016208:Databases, Factual; D004285:Dogs; D015180:Electrophoresis, Gel, Two-Dimensional; D006801:Humans; D008969:Molecular Sequence Data; D009206:Myocardium / Q000378:metabolism*; D010449:Peptide Mapping; D011506:Proteins / Q000378:metabolism*; D013045:Species Specificity
D011506:Proteins