[Functional expression of bovine retina adenylate cyclase b fragment cDNA in Escherichia Coli cells].
A 1488-bp fragment of bovine retina guanylate cyclase B gene encoding the catalytic and dimerizing domains as well as part of the protein kinase domain was expressed in Escherichia coli cells. The expression product was obtained as inclusion bodies and solubilized in 6 M guanidine hydrochloride. The fragment of guanylate cyclase B is a dimer close in catalytic activity to the native enzyme.
Solov'eva
O V
OV
Abdulaev
N G
NG
D000818:Animals; D002417:Cattle; D018076:DNA, Complementary / Q000737:chemistry / Q000235:genetics; D019281:Dimerization; D004591:Electrophoresis, Polyacrylamide Gel; D004926:Escherichia coli / Q000378:metabolism*; D015971:Gene Expression Regulation, Enzymologic; D005822:Genetic Vectors / Q000235:genetics; D019791:Guanidine / Q000737:chemistry; D006162:Guanylate Cyclase / Q000096:biosynthesis* / Q000235:genetics / Q000302:isolation & purification; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000235:genetics / Q000302:isolation & purification; D012160:Retina / Q000201:enzymology*
D018076:DNA, Complementary; D011994:Recombinant Proteins; D006162:Guanylate Cyclase; D019791:Guanidine
Funktsional'naia ekspressiia fragmenta kDNK guanilattsiklazy B setchatki byka v kletkakh Escherichia coli.