title: Purification and characterization of the malate dehydrogenase from Streptomyces aureofaciens.
abstract: The malate dehydrogenase (MDH) from Streptomyces aureofaciens was purified to homogeneity and its physical and biochemical properties were studied. Its amino-terminal sequence perfectly matched the amino-terminal sequence of the MDH from Streptomyces atratus whose biochemical characteristics have never been determined. The molecular mass of the native enzyme, estimated by size-exclusion chromatography, was 70 kDa. The protein was a homodimer, with a 38-kDa subunit molecular mass. It showed a strong specificity for NADH and was much more efficient for the reduction of oxaloacetate than for the oxidation of malate, with a pH optimum of 8. Unlike MDHs from other sources, it was not inhibited by excess oxaloacetate. This first complete functional characterization of an MDH from Streptomyces shows that the enzyme is very similar in many respects to other bacterial MDHs with the notable exception of a lack of inhibition by excess substrate.
authors.affiliation: Department of Biochemistry, Faculty of Sciences, Comenius University, Bratislava, Slovak Republic.
authors.forename: D
authors.initials: D
authors.lastname: Mikulásová
authors.forename: M
authors.initials: M
authors.lastname: Kollárová
authors.forename: M
authors.initials: M
authors.lastname: Miginiac-Maslow
authors.forename: P
authors.initials: P
authors.lastname: Decottignies
authors.forename: J P
authors.initials: JP
authors.lastname: Jacquot
authors.forename: E
authors.initials: E
authors.lastname: Kutejová
authors.forename: N
authors.initials: N
authors.lastname: Mernik
authors.forename: I
authors.initials: I
authors.lastname: Egyudová
authors.forename: R
authors.initials: R
authors.lastname: Musrati
authors.forename: T
authors.initials: T
authors.lastname: Horecká
mesh_terms: D000595:Amino Acid Sequence; D007700:Kinetics; D008291:Malate Dehydrogenase / Q000302:isolation & purification* / Q000378:metabolism; D008969:Molecular Sequence Data; D008970:Molecular Weight; D013304:Streptomyces aureofaciens / Q000201:enzymology*
chemical_list: D008291:Malate Dehydrogenase