title: Comparison of the productivity of cefoperazone amphotericin teicoplanin (CAT) agar and modified charcoal cefoperazone deoxycholate (mCCD) agar for various strains of Campylobacter, Arcobacter and Helicobacter pullorum.
abstract: Cefoperazone amphotericin teicoplanin (CAT) agar was developed from cefoperazone deoxycholate (mCCD) agar by modification of the selective antibiotics in order to permit growth of strains of Campylobacter upsaliensis. In this study, 35 strains of Campylobacter and Arcobacter were tested for their ability to grow on CAT and mCCD media using the ecometric method. Six of these strains were also tested using the modified Miles-Misra method. Overall, nineteen strains out of the 35 tested grew better on CAT than on mCCD agar, although for eight strains, the difference was slight. These differences could not be attributed solely to poorer growth of C. upsaliensis on mCCD agar. No strain grew better on mCCD than CAT agar. Eight of the 35 strains tested did not grow on mCCD agar at all, however, only one strain failed to grow on CAT medium. The two methods of testing gave similar results, although the Miles-Misra method was found to be more sensitive and less prone to subjective interpretation. All four CNUPC (catalase negative, urease positive campylobacter-like) strains, one strain of C. sputorum biovar, fecalis, one of two Arcobacter cryaerophilus strains (incubated at 30 degrees C, aerobically) could be detected only using CAT agar. In addition, for some strains of A. butzleri, C. upsaliensis and C. hyointestinalis, CAT medium gave better growth scores than mCCD agar. The level of cefoperazone in mCCDA is inhibitory to some campylobacter strains, but suboptimal growth of Arcobacter strains is more probably due to synergistic interaction between deoxycholate and cefoperazone. CAT agar supports the growth of a wider variety of Campylobacter and Arcobacter species than mCCD agar.
authors.affiliation: Department of Clinical Veterinary Science, University of Bristol, Langford, North Somerset, UK. janet.corry@bris.ac.uk
authors.forename: J E
authors.initials: JE
authors.lastname: Corry
authors.forename: H I
authors.initials: HI
authors.lastname: Atabay
mesh_terms: D000362:Agar; D000666:Amphotericin B / Q000494:pharmacology*; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D002167:Campylobacter / Q000187:drug effects / Q000254:growth & development*; D002438:Cefoperazone / Q000494:pharmacology*; D003840:Deoxycholic Acid / Q000494:pharmacology*; D016998:Helicobacter / Q000187:drug effects / Q000254:growth & development*; D008826:Microbial Sensitivity Tests; D017334:Teicoplanin / Q000494:pharmacology*
chemical_list: D000900:Anti-Bacterial Agents; D003840:Deoxycholic Acid; D017334:Teicoplanin; D002438:Cefoperazone; D000666:Amphotericin B; D000362:Agar