Simplified technique for detection of significant bacteriuria by microscopic examination of urine.
A comparative study of microscopic examination of 10 microl (simplified loop technique) and 50 microl (traditional drop technique) of uncentrifuged Gram-stained urine specimens for detection of significant bacteriuria was carried out. The results demonstrated that the 10-microl loop technique can be used as an alternative to the 50-microl drop technique for presumptive diagnosis of urinary-tract infection in bacteriological practice, with the advantages of greater rapidity and ease of performance.
Cardoso
C L
CL
Department of Clinical Analyses, State University of Maringá, Paraná, Brazil. deres@cybertelecom.com.br
Muraro
C B
CB
Siqueira
V L
VL
Guilhermetti
M
M
D001431:Bacteriological Techniques; D001437:Bacteriuria / Q000175:diagnosis*; D005840:Gentian Violet; D006801:Humans; D010619:Phenazines; D011237:Predictive Value of Tests; D012680:Sensitivity and Specificity; D013194:Staining and Labeling; D014556:Urine / Q000382:microbiology*
C035863:Gram's stain; D010619:Phenazines; D005840:Gentian Violet