title: Separate and variably shaped chromosome arm domains are disclosed by chromosome arm painting in human cell nuclei.
abstract: Fluorescence in situ hybridization (FISH) with microdissection probes from human chromosomes 3 and 6 was applied to visualize arm and subregional band domains in human amniotic fluid cell nuclei. Confocal laser scanning microscopy and quantitative three-dimensional image analysis showed a pronounced variability of p- and q-arm domain arrangements and shapes. Apparent intermingling of neighbouring arm domains was limited to the domain surface. Three-dimensional distance measurements with pter and qter probes supported a high variability of chromosome territory folding.
authors.affiliation: Institute of Human Genetics, University of Heidelberg, Germany. dietzel@wotan.iwr.uni-heidelberg.de
authors.forename: S
authors.initials: S
authors.lastname: Dietzel
authors.forename: A
authors.initials: A
authors.lastname: Jauch
authors.forename: D
authors.initials: D
authors.lastname: Kienle
authors.forename: G
authors.initials: G
authors.lastname: Qu
authors.forename: H
authors.initials: H
authors.lastname: Holtgreve-Grez
authors.forename: R
authors.initials: R
authors.lastname: Eils
authors.forename: C
authors.initials: C
authors.lastname: Münkel
authors.forename: M
authors.initials: M
authors.lastname: Bittner
authors.forename: P S
authors.initials: PS
authors.lastname: Meltzer
authors.forename: J M
authors.initials: JM
authors.lastname: Trent
authors.forename: T
authors.initials: T
authors.lastname: Cremer
mesh_terms: D000653:Amniotic Fluid / Q000166:cytology; D002478:Cells, Cultured; D002893:Chromosomes, Human, Pair 3; D002896:Chromosomes, Human, Pair 6; D015342:DNA Probes; D005260:Female; D006801:Humans; D007091:Image Processing, Computer-Assisted; D017404:In Situ Hybridization, Fluorescence / Q000379:methods*; D007399:Interphase; D008214:Lymphocytes; D008297:Male; D008677:Metaphase; D018613:Microscopy, Confocal
chemical_list: D015342:DNA Probes