title: [Detection of mycobacterium tuberculosis DNA using nested polymerase chain reaction in lymph nodes with sarcoidosis, fixed in formalin and embedded in paraffin].
abstract: An involvement of mycobacteria in the pathogenesis of sarcoidosis has often been hypothesized, but not confirmed reproducibly. In this study we applied a nested Polymerase Chain Reaction (PCR) to the insertion sequence IS6110 for detection of Mycobacterium tuberculosis (MT) DNA in formalin-fixed and paraffin-embedded tissues from patients with sarcoidosis, with tuberculosis and atypical mycobacteriosis confirmed by culture, and from negative control samples. MT-DNA could be detected in 2/30 samples of sarcoidosis, in 10/10 tuberculoses, in 0/5 atypical mycobacterioses and in 0/10 negative controls. Nested PCR confirmed its high sensitivity and specificity in detecting MT-DNA on archival histopathological specimens. From our results we conclude that in the granulomatous lesions of sarcoidosis MT-DNA is only sporadically demonstrable and probably it doesn't play a role in the pathogenesis of this disease.
authors.affiliation: Istituto di Anatomia e Istologia Patologica, Università di Milano, Ospedale Luigi Sacco.
authors.forename: M
authors.initials: M
authors.lastname: Cannone
authors.forename: L
authors.initials: L
authors.lastname: Vago
authors.forename: G
authors.initials: G
authors.lastname: Porini
authors.forename: S
authors.initials: S
authors.lastname: Bonetto
authors.forename: C
authors.initials: C
authors.lastname: Cassi
authors.forename: M
authors.initials: M
authors.lastname: Bramerio
authors.forename: G
authors.initials: G
authors.lastname: Rizzato
authors.forename: M C
authors.initials: MC
authors.lastname: Barberis
mesh_terms: D004269:DNA, Bacterial / Q000032:analysis*; D005404:Fixatives; D005557:Formaldehyde; D006801:Humans; D008198:Lymph Nodes / Q000382:microbiology* / Q000473:pathology; D015270:Mycobacterium avium-intracellulare Infection / Q000150:complications / Q000382:microbiology / Q000473:pathology; D009169:Mycobacterium tuberculosis / Q000235:genetics / Q000302:isolation & purification*; D016612:Paraffin Embedding; D016133:Polymerase Chain Reaction; D012507:Sarcoidosis / Q000150:complications / Q000382:microbiology* / Q000473:pathology; D012680:Sensitivity and Specificity; D016707:Tissue Fixation; D014388:Tuberculosis, Lymph Node / Q000150:complications / Q000382:microbiology / Q000473:pathology
chemical_list: D004269:DNA, Bacterial; D005404:Fixatives; D005557:Formaldehyde
vernacular_title: Ricerca del DNA del Mycobacterium tuberculosis mediante nested Polymerase Chain Reaction in linfonodi con sarcoidosi fissati in formalina e inclusi in paraffina.