HIV-1/simian immunodeficiency virus infection of human and nonhuman primate lymphocytes results in the migration of CD2+ T cells into the intestine of engrafted SCID mice.
Increased lymphocytic infiltration of intestinal tissues has been observed in patients infected with HIV-1 and in SIV-infected rhesus macaques. To determine whether HIV-1 and SIV infections influence the homing of human and nonhuman primate PBMC to intestinal tissues, we engrafted SCID mice with human or nonhuman primate PBMC and infected them with either cell-free or cell-associated HIV-1 or SIV. In mice that received both PBMC and virus, human or nonhuman primate CD2+ T cells were found in intestinal tissues, primarily in the intraepithelial lymphocyte compartment and lamina propria. Immunomagnetic sorting revealed that these cells were derived from the CD4+ population. Using gag-specific primers, PCR analysis of these tissues detected the presence of HIV-1 proviral DNA. However, in SCID mice that were engrafted with either human or nonhuman primate PBMC and no HIV-1 or SIV, CD2+ T cells were not detected in intestinal tissues. These results indicate that HIV-1 and SIV can modulate the migratory properties of human and nonhuman primate T cells in the SCID mouse model.
Donze
H H
HH
Department of Microbiology, University of Alabama, Birmingham 35294, USA.
Cummins
J E
JE
Schwiebert
R S
RS
Kantele
A
A
Han
Y
Y
Fultz
P N
PN
Jackson
S
S
Mestecky
J
J
D000818:Animals; D018801:CD2 Antigens / Q000032:analysis*; D016516:CD4-CD8 Ratio; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology; D002465:Cell Movement / Q000276:immunology*; D002478:Cells, Cultured; D004279:DNA, Viral / Q000032:analysis; D005260:Female; D015488:HIV Antigens / Q000032:analysis; D015658:HIV Infections / Q000276:immunology* / Q000473:pathology; D015497:HIV-1 / Q000276:immunology; D006801:Humans; D009980:Influenza A virus / Q000276:immunology; D007274:Injections, Intraperitoneal; D007413:Intestinal Mucosa / Q000276:immunology* / Q000473:pathology / Q000821:virology; D017710:Lymphocyte Transfusion; D008253:Macaca mulatta; D008254:Macaca nemestrina; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D016513:Mice, SCID; D002679:Pan troglodytes; D010529:Peritoneal Cavity / Q000473:pathology; D011533:Proviruses / Q000235:genetics; D016511:Severe Combined Immunodeficiency / Q000235:genetics / Q000276:immunology; D016097:Simian Acquired Immunodeficiency Syndrome / Q000276:immunology* / Q000473:pathology; D015302:Simian Immunodeficiency Virus / Q000235:genetics / Q000276:immunology; D016176:T-Lymphocyte Subsets / Q000276:immunology* / Q000473:pathology
D018801:CD2 Antigens; D004279:DNA, Viral; D015488:HIV Antigens