Different expression pattern of cytokine receptors by human osteosarcoma cell lines.
Cytokine receptor expression in human osteosarcoma cell lines (U2-OS, Saos-2, MG-63) was analyzed by flow cytometry to identify receptors which may interact with osteosarcoma cell growth and that should not be used in a clinical setting. U2-OS, Saos-2, MG-63 and bone marrow stromal cells, that were used as normal controls, constitutively express the FAS and SCFR surface molecules. GM-CSFR is expressed only by U2-OS and Saos-2 cell lines, that are phenotypically less differentiated than MG-63. Different gp130 clones were express ed only by Saos-2 and MG-63 cell lines. IL-2Rgamma,IL-7R and 4-1BB were expressed only by Saos-2 cell line. These data add new evidence of receptors that may be activated by autocrine or paracrine cytokines that could induce osteosarcoma cell growth.
Lisignoli
G
G
Laboratorio di Immunologia e Genetica, Istituti Ortopedici Rizzoli, Via di Barbiano 1/10, 40136 Bologna, Italy.
Toneguzzi
S
S
Cattini
L
L
Pozzi
C
C
Facchini
A
A
D001859:Bone Neoplasms / Q000737:chemistry*; D005434:Flow Cytometry; D006801:Humans; D012516:Osteosarcoma / Q000737:chemistry*; D018121:Receptors, Cytokine / Q000032:analysis*; D014407:Tumor Cells, Cultured
D018121:Receptors, Cytokine