Structure of a neutral O-specific polysaccharide of the bacterium Proteus mirabilis O24.
Lipopolysaccharide of the bacterium Proteus mirabilis O24 was found to have a neutral O-specific polysaccharide chain containing D-galactose, 2-acetamido-2-deoxy-D-glucose, and 2-acetamido-2-deoxy-D-galactose in ratios 1:2:1. On the basis of 1H- and 13C-NMR spectroscopy, including two-dimensional correlation spectroscopy (COSY), H-detected 1H, 13C heteronuclear multiple-quantum coherence (HMQC), and nuclear Overhauser effect spectroscopy (NOESY), the following structure of the branched tetrasaccharide repeating unit of the O-specific polysaccharide was established: -->3)-beta-D-GlcpNAc-(1-->4)-beta-D-GalpNAc-(1-->4)-beta-D-GlcpNAc-(1--> [formula: see text] beta-D-Galp.
Senchenkova
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Zelinskii Institute of Organic Chemistry, Russian Academy of Sciences, Moscow, Russia.
Zatonskii
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Ujazda
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Shashkov
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Kaca
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Knirel YuA
D002236:Carbohydrate Conformation; D009682:Magnetic Resonance Spectroscopy; D019081:O Antigens / Q000737:chemistry*; D011513:Proteus mirabilis / Q000737:chemistry*
D019081:O Antigens