Response of human osteoblasts to polymethylmetacrylate In vitro.
The effects of a polymethylmetacrylate (PMMA) powder with a diameter between 0.5 and 25 mu m have been studied in vitro on several human osteoblast populations obtained from different sources. Parameters of cell activity such as cell growth, collagen synthesis, osteocalcin, and interleukin-6 (IL-6) production have been evaluated. Cell proliferation and collagen synthesis were inhibited after exposure to bone cement, whereas osteocalcin and IL-6 production were stimulated. These results suggest that PMMA particles could affect osteoblast activity in a way that could contribute, together with other factors, to periprosthetic osteolysis through two different pathways: a reduced periprosthetic bone formation due to the reduced osteoblast proliferation and collagen synthesis, and an osteoblast-mediated activation of osteoclastic bone resorption as suggested by the increased osteocalcin and IL-6 synthesis. In fact, osteocalcin has been demonstrated to have a role in osteoclast recruitment to bone surfaces, and IL-6 is known to induce osteoclastogenesis and to directly stimulate bone resorption.
Zambonin
G
G
Ist Orthopaedic Clinic, University of Bari, Bari, Italy.
Colucci
S
S
Cantatore
F
F
Grano
M
M
D001843:Bone Cements / Q000494:pharmacology*; D006801:Humans; D015850:Interleukin-6 / Q000378:metabolism; D010006:Osteoblasts / Q000166:cytology / Q000187:drug effects*; D015675:Osteocalcin / Q000187:drug effects / Q000378:metabolism; D019904:Polymethyl Methacrylate / Q000494:pharmacology*; D011392:Proline / Q000378:metabolism; D013936:Thymidine / Q000378:metabolism; D014316:Tritium
D001843:Bone Cements; D015850:Interleukin-6; D014316:Tritium; D015675:Osteocalcin; D019904:Polymethyl Methacrylate; D011392:Proline; D013936:Thymidine