Immunohistochemical colocalization of serotonin, substance P and Met-enkephalin-Arg6-Gly7-Leu8 in the endocrine cells of the ruminant duodenum.
The duodenum of various ruminants was examined by immunohistochemical staining for serotonin, substance P and Metenkephalin-Arg6-Gly7-Leu8 (MENK8). Serotonin- and substance P-immunoreactive endocrine cells were detected in samples from cow, calf, sheep, goat, and barbary sheep, MENK8-immunoreactive endocrine cells were detected exclusively in samples from cow and calf. Substance P and MENK8 immunoreactivity was found in serotonin-immunoreactive endocrine cells of cattle. Substance P- and MENK8-immunoreactive nervous elements were detected in all ruminants examined. The present results suggest that the expression and/or the mechanism that controls the expression of each peptide might differ between endocrine cells and nerve cells and might also depend on animal species.
Mimoda
T
T
Department of Veterinary Anatomy, Obihiro University of Agriculture and Veterinary Medicine, Japan.
Kitamura
N
N
Hondo
E
E
Yamada
J
J
D000375:Aging; D000818:Animals; D002417:Cattle; D004386:Duodenum / Q000166:cytology*; D004744:Enkephalin, Methionine / Q000031:analogs & derivatives* / Q000032:analysis; D004759:Enterochromaffin Cells / Q000166:cytology*; D006041:Goats; D007150:Immunohistochemistry / Q000379:methods; D007413:Intestinal Mucosa / Q000166:cytology*; D012701:Serotonin / Q000032:analysis*; D012756:Sheep; D013045:Species Specificity; D013373:Substance P / Q000032:analysis*
D012701:Serotonin; D013373:Substance P; D004744:Enkephalin, Methionine; C033453:enkephalin-Met, Arg(6)-Gly(7)-Leu(8)-