[Spectrophotometric study of the interaction of single-stranded RNA with tilorone].
The spectrophotometric study of complex formation between phage MS2 single-stranded RNA and tilorone has been carried out. It was found that with components concentration forming "strong linkage" the absorption spectra of RNA-tilorone complex in UV-region experience hyperchromic and bathochromic shifts; besides, a new absorption maximum appears at 280 nm. The changes of spectral properties have been also detected in the long-waved region of the complex spectra. The conclusion has been made that in the course of single-stranded RNA with tilorone interactions a complex formation takes place due to interacalation between spontaneously appeared two-stranded regions in the structure of a single-stranded RNA and tilorone; which component stabilizes these structures and assures there by the previously described interferonogenic activity of a single-stranded RNA--tilorone complexes.
Karpov
O V
OV
D.K. Zabolotny Institute of Microbiology and Virology, National Academy of Sciences of Ukraine, Kyiv.
D000998:Antiviral Agents / Q000494:pharmacology*; D015394:Molecular Structure; D012367:RNA, Viral / Q000187:drug effects*; D013053:Spectrophotometry / Q000379:methods; D013056:Spectrophotometry, Ultraviolet; D013994:Tilorone / Q000494:pharmacology*
D000998:Antiviral Agents; D012367:RNA, Viral; D013994:Tilorone
Spektrofotometrychne vyvchennia vzaiemodiï odnolantsiuhovoï RNK z tyloronom.