Structural determination of the O-antigenic polysaccharide from the enterotoxigenic Escherichia coli O147.
The structure of the O-antigenic polysaccharide from enterotoxigenic Escherichia coli O147 has been determined by NMR spectroscopy, and component and methylation analyses. The sequence of the sugar residues could be determined by NOESY and heteronuclear-multiple-bond-connectivity NMR experiments. It is concluded that the polysaccharide is composed of tetrasaccharide repeating units with the following structure: -->4)-beta-D-GalpA-(1-->3)-beta-D-GalpNAc-(1-->2)-alpha-L-Rhap+ ++-(1-->2)-alpha-L-Rhap-(1-->, where Rha represents 6-deoxymannose. The O-antigen of E. coli O147 is identical to the repeating unit of Shigella flexneri serotype 6 lipopolysaccharide, except that the latter contains an O-acetyl group at C3 of the rhamnosyl residue substituted by the N-acetylgalactosamine residue. Immunochemical analyses using a monoclonal antibody specific for the S. flexneri serotype 6 O-antigen showed an identical reactivity with both lipopolysaccharides.
Hygge Blakeman
K
K
Department of Organic Chemistry, Stockholm University, Sweden.
Weintraub
A
A
Widmalm
G
G
D002240:Carbohydrate Sequence; D002241:Carbohydrates / Q000032:analysis; D003429:Cross Reactions; D004926:Escherichia coli / Q000276:immunology* / Q000472:pathogenicity; D009682:Magnetic Resonance Spectroscopy; D008969:Molecular Sequence Data; D015394:Molecular Structure; D019081:O Antigens / Q000737:chemistry* / Q000276:immunology*; D012210:Rhamnose / Q000032:analysis / Q000737:chemistry; D012763:Shigella flexneri / Q000276:immunology
D002241:Carbohydrates; D019081:O Antigens; D012210:Rhamnose