Infection of K562 cells with influenza A virus increases their susceptibility to natural killer lysis.
Natural killer (NK) cells play a role in the natural immunity against tumor cells. In the present study, we demonstrate that infection of the NK-sensitive tumor cell line K562 with influenza A virus caused a substantial increase in lysis of up to sevenfold when compared to noninfected cells. Similar to NK cells, IL-2-activated killer cells exhibited higher lytic activity against virus-infected K562 cells. This effect of the virus correlated with the increase in the expression of intracellular adhesion molecule-1 (ICAM-1) on K562 cells. Changes in the susceptibility to NK lysis were accompanied by alterations, within minutes, in the cytoskeleton as detected by intracellular fluorescein fluorescence polarization measured on the Cellscan, a static cytometer. The possible role of iCAM-1 and the cytoskeleton in the cytotoxic response of NK cells is discussed.
Eisenthal
A
A
Tel-Aviv Sourasky Medical Center, Israel.
Marder
O
O
Lifschitz-Mercer
B
B
Skornick
Y
Y
Tirosh
R
R
Irlin
Y
Y
Avtalion
R
R
Deutsch
M
M
D003602:Cytotoxicity, Immunologic / Q000187:drug effects; D004198:Disease Susceptibility / Q000276:immunology; D005452:Fluoresceins; D005454:Fluorescence Polarization; D006801:Humans; D009980:Influenza A virus / Q000254:growth & development / Q000276:immunology*; D007251:Influenza, Human / Q000276:immunology* / Q000503:physiopathology; D018799:Intercellular Adhesion Molecule-1 / Q000096:biosynthesis / Q000276:immunology; D007376:Interleukin-2 / Q000494:pharmacology; D015979:Killer Cells, Lymphokine-Activated / Q000166:cytology / Q000187:drug effects / Q000276:immunology; D007694:Killer Cells, Natural / Q000187:drug effects / Q000276:immunology*; D007963:Leukocytes, Mononuclear / Q000187:drug effects / Q000276:immunology / Q000821:virology; D013997:Time Factors; D014407:Tumor Cells, Cultured / Q000821:virology; D014779:Virus Replication / Q000502:physiology
D005452:Fluoresceins; D007376:Interleukin-2; D018799:Intercellular Adhesion Molecule-1