title: Ganglioside composition of a mouse brain tumor grown in the severe combined immunodeficiency (SCID) mouse.
abstract: The content and composition of gangliosides were examined in an experimental mouse brain tumor, EPEN, that was grown subcutaneously in the flank of the syngeneic C57BL/6J (B6) host and in the B6 severe combined immunodeficiency (SCID) host. SCID mice lack functional T- and B-lymphocytes, but have a normal complement of macrophages. The content and distribution of the brain tumor gangliosides were similar whether the tumor was grown in the immunocompetent B6 host or in the B6-SCID host. N-acetylneuraminic acid- (NeuAc) containing GM3 was the major ganglioside in the subcutaneous tumors and in the cultured EPEN cells. Significant amounts of N-glycolylneuraminic acid- (NeuGc) containing gangliosides were found in the tumor grown in both mouse hosts. NeuGc-containing gangliosides are not expressed in normal mouse brain, but are present in macrophages and serum. An extremely complex pattern of minor gangliosides was found in the subcutaneous tumors on two-dimensional, high-performance thin-layer chromatograms. Most of the minor gangliosides comigrated with those found in mouse macrophages. The results show that the absence of functional T- and B-lymphocytes does not markedly affect brain tumor ganglioside composition and suggest that NeuGc-containing gangliosides in the EPEN can be derived from tumor infiltrating host cells (mostly macrophages) and from the extracellular milieu (serum).
authors.affiliation: Department of Biology, Boston College, Chestnut Hill, MA 02167-3811, USA.
authors.forename: T N
authors.initials: TN
authors.lastname: Seyfried
authors.forename: M
authors.initials: M
authors.lastname: el-Abbadi
authors.forename: J A
authors.initials: JA
authors.lastname: Ecsedy
authors.forename: M E
authors.initials: ME
authors.lastname: Griffin
authors.forename: H C
authors.initials: HC
authors.lastname: Yohe
mesh_terms: D000818:Animals; D001932:Brain Neoplasms / Q000737:chemistry* / Q000473:pathology; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D004806:Ependymoma / Q000737:chemistry* / Q000473:pathology; D005110:Extracellular Space / Q000378:metabolism; D005732:Gangliosides / Q000032:analysis* / Q000378:metabolism; D017737:Macrophages, Peritoneal / Q000378:metabolism; D051379:Mice; D008810:Mice, Inbred C57BL; D016513:Mice, SCID; D009928:Organ Specificity; D014183:Transplantation, Heterologous; D014407:Tumor Cells, Cultured
chemical_list: D005732:Gangliosides