title: PCR single strand conformational polymorphism can be used to detect the gene encoding SHV-7 extended-spectrum beta-lactamase and to identify different SHV genes within the same strain.
abstract: The PCR single strand conformational polymorphism (PCR-SSCP) technique described to identify mutants of the SHV beta-lactamases was extended to identify an SHV-7 type beta-lactamase. This was found in a strain of Klebsiella pneumoniae, the first recorded isolate in the UK to produce this type of enzyme. We also demonstrate that PCR-SSCP can be used to identify more than one SHV beta-lactamase gene in a single strain.
authors.affiliation: Department of Microbiology, University of Leeds, UK.
authors.forename: F H
authors.initials: FH
authors.lastname: M'Zali
authors.forename: J
authors.initials: J
authors.lastname: Heritage
authors.forename: D M
authors.initials: DM
authors.lastname: Gascoyne-Binzi
authors.forename: A M
authors.initials: AM
authors.lastname: Snelling
authors.forename: P M
authors.initials: PM
authors.lastname: Hawkey
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology; D004269:DNA, Bacterial / Q000737:chemistry; D005798:Genes, Bacterial; D007711:Klebsiella pneumoniae / Q000187:drug effects* / Q000201:enzymology; D008826:Microbial Sensitivity Tests; D009690:Nucleic Acid Conformation; D016133:Polymerase Chain Reaction; D018807:Polymorphism, Single-Stranded Conformational; D001618:beta-Lactamases / Q000235:genetics* / Q000378:metabolism
chemical_list: D000900:Anti-Bacterial Agents; D004269:DNA, Bacterial; D001618:beta-Lactamases