In vivo mapping of nucleosomes using psoralen-DNA crosslinking and primer extension.
By the use of psoralen crosslinking and primer extension, a method was developed which allows the analysis of chromatin structure in vivo. Using a yeast minichromosome, >9 nucleosomes were mapped with a resolution of at least +/-30 bp.
Wellinger
R E
RE
Institut fur Zellbiologie, ETH-Hönggerberg, CH-8093 Zurich, Switzerland.
Sogo
J M
JM
D001483:Base Sequence; D002874:Chromosome Mapping / Q000379:methods*; D015825:Chromosomes, Fungal / Q000235:genetics; D003432:Cross-Linking Reagents; D017931:DNA Primers / Q000235:genetics; D004271:DNA, Fungal / Q000235:genetics; D009707:Nucleosomes / Q000235:genetics*; D017319:Photosensitizing Agents; D012441:Saccharomyces cerevisiae / Q000235:genetics; D014307:Trioxsalen; D014466:Ultraviolet Rays
D003432:Cross-Linking Reagents; D017931:DNA Primers; D004271:DNA, Fungal; D009707:Nucleosomes; D017319:Photosensitizing Agents; D014307:Trioxsalen