title: Blockade of CD40-CD154 interferes with human T cell engraftment in scid mice.
abstract: Antibodies to the ligand for CD40 (CD154) have been shown to exert profound effects on the development of cell-mediated immune responses in mice. The present study shows that an antibody to human CD154 (hCD40L) inhibits in vivo Tetanus toxoid (TT) specific secondary antibody responses in hu-PBL-scid mice, as well as the expansion of xenoreactive human T cells in the scid mice. A possible cause for the reduced expansion of xenoreactive, human T cells, was the decreased expression of murine B7.1 and B7.2 caused by the administration of anti-hCD40L. Therefore, it may be that defective maturation of murine antigen-presenting cells impeded the priming and expansion of human xenoreactive T cells.
authors.affiliation: Department of Microbiology, Dartmouth Medical School, Lebanon, NH 03756, USA.
authors.forename: T M
authors.initials: TM
authors.lastname: Foy
authors.forename: M
authors.initials: M
authors.lastname: McIlraith
authors.forename: S R
authors.initials: SR
authors.lastname: Masters
authors.forename: J J
authors.initials: JJ
authors.lastname: Dunn
authors.forename: A A
authors.initials: AA
authors.lastname: Rossini
authors.forename: L D
authors.initials: LD
authors.lastname: Shultz
authors.forename: R A
authors.initials: RA
authors.lastname: Hesselton
authors.forename: E J
authors.initials: EJ
authors.lastname: Wagar
authors.forename: P E
authors.initials: PE
authors.lastname: Lipsky
authors.forename: R J
authors.initials: RJ
authors.lastname: Noelle
authors.forename: D L
authors.initials: DL
authors.lastname: Greiner
mesh_terms: D000328:Adult; D000818:Animals; D000911:Antibodies, Monoclonal; D015703:Antigens, CD / Q000378:metabolism; D001402:B-Lymphocytes / Q000166:cytology / Q000276:immunology; D018122:B7-1 Antigen / Q000378:metabolism; D051940:B7-2 Antigen; D019013:CD40 Antigens / Q000378:metabolism*; D023201:CD40 Ligand; D002454:Cell Differentiation; D017690:Cell Transplantation; D006085:Graft Survival / Q000276:immunology; D006801:Humans; D008024:Ligands; D008213:Lymphocyte Activation; D008562:Membrane Glycoproteins / Q000037:antagonists & inhibitors* / Q000378:metabolism; D051379:Mice; D016513:Mice, SCID; D013601:T-Lymphocytes / Q000276:immunology* / Q000637:transplantation*; D013745:Tetanus Toxoid / Q000276:immunology; D014183:Transplantation, Heterologous
chemical_list: D000911:Antibodies, Monoclonal; D015703:Antigens, CD; D018122:B7-1 Antigen; D051940:B7-2 Antigen; D019013:CD40 Antigens; C497964:CD86 protein, human; C497965:Cd86 protein, mouse; D008024:Ligands; D008562:Membrane Glycoproteins; D013745:Tetanus Toxoid; D023201:CD40 Ligand