title: Location of enhancers is essential for the imprinting of H19 and Igf2 genes.
abstract: Genomic imprinting is the process in mammals by which gamete-specific epigenetic modifications establish the differential expression of the two alleles of a gene. The tightly linked H19 and Igf2 genes are expressed in tissues of endodermal and mesodermal origin, with H19 expressed from the maternal chromosome and Igf2 expressed from the paternal chromosome. A model has been proposed to explain the reciprocal imprinting of these genes; in this model, expression of the genes is governed by competition between their promoters for a common set of enhancers. An extra set of enhancers might be predicted to relieve the competition, thereby eliminating imprinting. Here we tested this prediction by generating mice with a duplication of the endoderm-specific enhancers. The normally silent Igf2 gene on the maternal chromosome was expressed in liver, consistent with relief from competition. We then generated a maternal chromosome containing a single set of enhancers located equidistant from 1gf2 and H19; the direction of the imprint was reversed. Thus, the location of the enhancers determines the outcome of competition in liver, and the strength of the H19 promoter is not sufficient to silence Igf2.
authors.affiliation: Howard Hughes Medical Institute and Department of Molecular Biology, Princeton University, New Jersey 08544, USA.
authors.forename: A L
authors.initials: AL
authors.lastname: Webber
authors.forename: R S
authors.initials: RS
authors.lastname: Ingram
authors.forename: J M
authors.initials: JM
authors.lastname: Levorse
authors.forename: S M
authors.initials: SM
authors.lastname: Tilghman
mesh_terms: D000483:Alleles; D000818:Animals; D002460:Cell Line; D003433:Crosses, Genetic; D019175:DNA Methylation; D004742:Enhancer Elements, Genetic; D005260:Female; D018392:Genomic Imprinting; D007335:Insulin-Like Growth Factor II / Q000235:genetics*; D007668:Kidney / Q000378:metabolism; D008099:Liver / Q000378:metabolism; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D009115:Muridae; D009124:Muscle Proteins / Q000235:genetics*; D009132:Muscles / Q000378:metabolism; D016296:Mutagenesis; D062085:RNA, Long Noncoding; D022661:RNA, Untranslated; D013234:Stem Cells
chemical_list: C094335:H19 long non-coding RNA; D009124:Muscle Proteins; D062085:RNA, Long Noncoding; D022661:RNA, Untranslated; D007335:Insulin-Like Growth Factor II