Antimetastatic effect of NK1+ T cells on experimental haematogenous tumour metastases in the liver and lungs of mice.
Depletion of both natural killer 1.1+ (NK1+) intermediate alpha beta T-cell receptor (int T) cells and NK cells by in vivo treatment with anti-NK1 antibody greatly increased hepatic metastases of intravenously injected EL4 cells as well as pulmonary metastases of 3LL cells in C57BL/6 mice. However, depletion of NK cells alone by anti-asialo GM1 (AGM1) antibody treatment did not increase the metastases in either organ. Interleukin-12 (IL-12) administration into mice induced strong cytotoxicities of NK cell-depleted liver and lung mononuclear cells (MNC) comparable to those without NK-cell depletion and inhibited metastases in either organ. In contrast, in both NK cell- and NK1+ int T-cell-depleted mice, IL-12 could not induce cytotoxic activity of liver and lung MNC and metastases in both organs increased with or without IL-12 treatment. These results confirmed the fact that NK+ int T cells are more potent antitumour effectors than NK cells against experimental haematogenous tumour metastases.
Seki
S
S
Division of Basic Traumatology, National Defence Medical College Research Institute, Tokorozawa, Japan.
Hashimoto
W
W
Ogasawara
K
K
Satoh
M
M
Watanabe
H
H
Habu
Y
Y
Hiraide
H
H
Takeda
K
K
D000818:Animals; D003602:Cytotoxicity, Immunologic; D005434:Flow Cytometry; D005677:G(M1) Ganglioside / Q000378:metabolism; D018664:Interleukin-12 / Q000276:immunology; D007694:Killer Cells, Natural / Q000276:immunology*; D007963:Leukocytes, Mononuclear / Q000276:immunology; D008113:Liver Neoplasms / Q000276:immunology / Q000556:secondary*; D008175:Lung Neoplasms / Q000276:immunology / Q000556:secondary*; D008213:Lymphocyte Activation / Q000276:immunology; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D009368:Neoplasm Transplantation; D016176:T-Lymphocyte Subsets / Q000276:immunology*
D018664:Interleukin-12; D005677:G(M1) Ganglioside; C018835:asialo GM1 ganglioside