Modulation of immunologic responses to HIV-1MN recombinant gp160 vaccine by dose and schedule of administration. National Institute of Allergy and Infectious Diseases AIDS Vaccine Evaluation Group.
The safety and immunogenicity of HIV-1MN recombinant gp160 (MN rgp160) vaccine in healthy, uninfected volunteers was tested in a double-blind study with a factorial design. By random assignment, 20 volunteers received three 200 micrograms doses of MN rgp160 and four volunteers received placebo at days 0, 28, and 168 or 0, 56, and 224. Of the 24 volunteers, 16 received 200 micrograms or 800 micrograms of MN rgp160 and two received placebo at day 532 (month 18). The vaccine was safe. It induced T cell memory measured by Th1 cytokine production and lymphocyte proliferation, and serum anti-MN rgp160 IgG (all subclasses) and IgA antibodies. Fifteen of 20 vaccinees developed neutralizing antibody. The regimen including immunizations on days 0, 28, and 168 followed by the 800 micrograms fourth dose was most immunogenic.
Gorse
G J
GJ
Division of Infectious Diseases and Immunology, Saint Louis University School of Medicine, MO, USA.
McElrath
M J
MJ
Matthews
T J
TJ
Hsieh
R H
RH
Belshe
R B
RB
Corey
L
L
Frey
S E
SE
Kennedy
D J
DJ
Walker
M C
MC
Eibl
M M
MM
D016915:AIDS Vaccines / Q000009:adverse effects*; D000293:Adolescent; D000328:Adult; D000914:Antibodies, Viral / Q000096:biosynthesis*; D000937:Antigen-Antibody Reactions; D002455:Cell Division; D016207:Cytokines / Q000096:biosynthesis; D004306:Dose-Response Relationship, Immunologic; D004311:Double-Blind Method; D005260:Female; D019302:HIV Envelope Protein gp160 / Q000276:immunology*; D018023:HIV Seronegativity; D006801:Humans; D007115:Immunization Schedule; D008214:Lymphocytes / Q000166:cytology; D008297:Male; D008875:Middle Aged; D011994:Recombinant Proteins / Q000276:immunology; D014614:Vaccines, Synthetic / Q000009:adverse effects*
D016915:AIDS Vaccines; D000914:Antibodies, Viral; D016207:Cytokines; D019302:HIV Envelope Protein gp160; D011994:Recombinant Proteins; D014614:Vaccines, Synthetic