title: 4-Aminobiphenyl-DNA adducts in laryngeal tissue and smoking habits: an immunohistochemical study.
abstract: 4-Aminobiphenyl (4-ABP)-DNA adducts and p53 overexpression were evaluated in laryngeal biopsies from 38 patients by immunohistochemical methods. Samples were categorized as tumors (n = 9), polyps (n = 28) or normal tissue (n = 1). 4-ABP-DNA adducts were evaluated with a quantitative immunoperoxidase method using monoclonal antibody 3C8 in both the lesion and adjacent tissue. Relative staining intensity data showed a log-normal distribution and values found in adjacent tissue from smokers were significantly higher (median: 173.5, geometric mean: 159.9) than those measured in adjacent tissue from non-smokers (median: 75.5, geometric mean: 7.40). Statistical significance was assessed both by non-parametric testing on raw data (P = 0.0007 on rank sum test) and by parametric testing on log-transformed data (P = 0.0002 on an unpaired t-test). Furthermore, relative staining intensity in the lesional tissue showed the same significant difference between smokers and non-smokers in patients affected by polyps, whereas no significant difference was detected in patients with laryngeal tumors. Overexpression of p53, also measured with an immunoperoxidase method, was observed in 44% of the malignant tumors and in 3.5% of the polyps. This work demonstrates that 4-ABP-DNA adducts can be evaluated in laryngeal tissue and are related to smoking exposure.
authors.affiliation: Institute of General Pathology-Centro di Ricerche Oncologiche Giovanni XXIII, Catholic University School of Medicine, Largo F. Vito, Rome, Italy. ibipg@rm.unicatt.it
authors.forename: G
authors.initials: G
authors.lastname: Flamini
authors.forename: G
authors.initials: G
authors.lastname: Romano
authors.forename: G
authors.initials: G
authors.lastname: Curigliano
authors.forename: A
authors.initials: A
authors.lastname: Chiominto
authors.forename: G
authors.initials: G
authors.lastname: Capelli
authors.forename: A
authors.initials: A
authors.lastname: Boninsegna
authors.forename: C
authors.initials: C
authors.lastname: Signorelli
authors.forename: L
authors.initials: L
authors.lastname: Ventura
authors.forename: R M
authors.initials: RM
authors.lastname: Santella
authors.forename: A
authors.initials: A
authors.lastname: Sgambato
authors.forename: A
authors.initials: A
authors.lastname: Cittadini
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000611:Aminobiphenyl Compounds / Q000378:metabolism*; D018736:DNA Adducts / Q000378:metabolism*; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D007822:Laryngeal Neoplasms / Q000378:metabolism*; D007830:Larynx / Q000378:metabolism; D008297:Male; D008875:Middle Aged; D011127:Polyps / Q000378:metabolism*; D012907:Smoking / Q000378:metabolism*; D016159:Tumor Suppressor Protein p53 / Q000378:metabolism*
chemical_list: D000611:Aminobiphenyl Compounds; D018736:DNA Adducts; D016159:Tumor Suppressor Protein p53; C006757:4-biphenylamine