title: Twist and Notch negatively regulate adult muscle differentiation in Drosophila.
abstract: Twist is required in Drosophila embryogenesis for mesodermal specification and cell-fate choice. We have examined the role of Twist and Notch during adult indirect flight muscle development. Reduction in levels of Twist leads to abnormal myogenesis. Notch reduction causes a similar mutant phenotype and reduces Twist levels. Conversely, persistent expression, in myoblasts, of activated Notch causes continued twist expression and failure of differentiation as assayed by myosin expression. The gain-of-function phenotype of Notch is very similar to that seen upon persistent twist expression. These results point to a relationship between Notch function and twist regulation during indirect flight muscle development and show that decline in Twist levels is a requirement for the differentiation of these muscles, unlike the somatic muscles of the embryo.
authors.affiliation: National Centre for Biological Sciences, Bangalore, India.
authors.forename: S
authors.initials: S
authors.lastname: Anant
authors.forename: S
authors.initials: S
authors.lastname: Roy
authors.forename: K
authors.initials: K
authors.lastname: VijayRaghavan
mesh_terms: D000818:Animals; D002454:Cell Differentiation; D029721:Drosophila Proteins; D004331:Drosophila melanogaster / Q000166:cytology / Q000196:embryology / Q000502:physiology*; D004625:Embryo, Nonmammalian / Q000502:physiology; D005426:Flight, Animal; D018507:Gene Expression Regulation, Developmental; D008565:Membrane Proteins / Q000096:biosynthesis / Q000502:physiology*; D009132:Muscles / Q000166:cytology / Q000502:physiology; D009218:Myosins / Q000096:biosynthesis; D009687:Nuclear Proteins / Q000096:biosynthesis / Q000502:physiology*; D010641:Phenotype; D011679:Pupa; D011956:Receptors, Cell Surface / Q000502:physiology*; D051880:Receptors, Notch; D014157:Transcription Factors; D051879:Twist-Related Protein 1; D014921:Wings, Animal / Q000166:cytology / Q000502:physiology
chemical_list: D029721:Drosophila Proteins; D008565:Membrane Proteins; C497759:N protein, Drosophila; D009687:Nuclear Proteins; D011956:Receptors, Cell Surface; D051880:Receptors, Notch; D014157:Transcription Factors; C497753:Twi protein, Drosophila; D051879:Twist-Related Protein 1; D009218:Myosins