ELISA for detection of antibody to the E2 protein of GB virus C.
An enzyme linked immunosorbent assay (ELISA) for detection of antibodies to mammalian cell-expressed E2 protein of GB virus C (GBV-C E2) is described. Antibodies to GBV-C E2 are captured on a solid phase coated with affinity purified E2 protein. Bound antibody is detected in an indirect assay format using horseradish peroxidase (HRPO) labeled goat anti-human IgG as the secondary antibody. Following a color development step, absorbance at 492 nm is measured. A population of 100 volunteer blood donors was tested to assess the specificity of this assay. Individuals reactive for antibody to GBV-C E2 can be considered to have been exposed to GB virus C.
Gutierrez
R A
RA
Experimental Biology Research, Abbott Diagnostics Division, Department 90D, Abbott Laboratories, Chicago, IL 60064-4000, USA. robin.gutierrez@ADD.SSW.ABBOTT.COM
Dawson
G J
GJ
Mushahwar
I K
IK
D001782:Blood Donors; D004797:Enzyme-Linked Immunosorbent Assay; D018067:Flaviviridae / Q000276:immunology*; D006508:Hepatitis Antibodies / Q000097:blood*; D006735:Horseradish Peroxidase; D006801:Humans; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013053:Spectrophotometry; D014759:Viral Envelope Proteins / Q000276:immunology*
D006508:Hepatitis Antibodies; D014759:Viral Envelope Proteins; C107619:glycoprotein E2, GB virus C; D006735:Horseradish Peroxidase