Porcine reproductive and respiratory syndrome virus infection of gnotobiotic pigs: sites of virus replication and co-localization with MAC-387 staining at 21 days post-infection.
The organ distribution of PRRSV-infected cells in gnotobiotic piglets at 21 days after infection with PRRSV isolate VR-2332 was examined by in situ hybridization. Cells that expressed PRRSV RNA were identified in all tissues examined, including organs not usually characterized as sites of PRRSV infection. PRRSV-infected cells frequently appeared in clusters and were not always associated with microscopic lesions. The expression of PRRSV RNA co-localized with a macrophage monoclonal antibody, MAC-387, in lymph nodes. Some, but not all infected cells stained with MAC-387. The wide distribution of PRRSV-infected cells and co-localization with MAC-387 staining is consistent with the macrophage-tropism of PRRSV and is similar to observations made during persistent infection with other arteriviruses.
Lawson
S R
SR
Department of Biology-Microbiology, South Dakota State University, Brookings 57007, USA.
Rossow
K D
KD
Collins
J E
JE
Benfield
D A
DA
Rowland
R R
RR
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D005856:Germ-Free Life; D017403:In Situ Hybridization; D008264:Macrophages / Q000276:immunology / Q000821:virology*; D019318:Porcine Reproductive and Respiratory Syndrome / Q000473:pathology / Q000821:virology*; D012367:RNA, Viral; D013194:Staining and Labeling; D013552:Swine; D014779:Virus Replication
D000911:Antibodies, Monoclonal; D012367:RNA, Viral