Immunogenic PDGF-AB dimers in crude leukocyte interferon batches.
The infection of human peripheral blood leukocytes (hPBL) with Sendai virus in vitro, induces the synthesis of human leukocyte interferons (IFN-alpha n3) as well as other cytokines. The authors discovered that supernatants of virus-infected hPBL also contained significant amounts of platelet-derived growth factor AB (PDGF-AB). The concentrations of immunogenic PDGF-AB in the interferon batches were determined by quantitative enzyme-linked immunosorbent assay (ELISA) before and after the inactivation of Sendai virus by acidification (pH 2) for 5 days. Immunogenic PDGF-AB molecules were detected in all interferon batches before and after acidification. The virus inactivation process caused a significant change in the content of immunogenic PDGF-AB in the interferon samples. Inactivation of the Sendai virus by acidification favoured the appearance of PDGF-AB dimers in the majority of samples.
Zidovec
S
S
Institute of Immunology, Zagreb, Croatia.
Mazuran
R
R
D019281:Dimerization; D006801:Humans; D007963:Leukocytes, Mononuclear / Q000378:metabolism* / Q000821:virology; D010982:Platelet-Derived Growth Factor / Q000378:metabolism*; D018108:Respirovirus / Q000502:physiology*
D010982:Platelet-Derived Growth Factor; C095477:platelet-derived growth factor AB