Expression of A and H blood-group and of CD44 antigens during chemical rat colonic carcinogenesis.
Using an experimental model of rat colon adenocarcinoma, we have recently shown that the presence of H blood-group antigen on variants of the CD44 adhesion molecule carrying amino acids encoded by exon v6 (CD44v6), increased the cells' tumorigenicity. In the present study, colon adenocarcinomas were induced by 1,2-dimethylhydrazine treatment in rats. Using immunohistochemistry, biopsies of normal, precancerous and carcinomatous colon mucosa were evaluated for expression A and H blood group antigens and CD44s and CD44v6 antigens. Normal rat colon showed strong and homogeneous expression of blood-group antigen A, but weak expression of H antigen. Several weeks before the appearance of tumours, dysplastic glands were strongly stained with anti-H reagents, while their A antigen was lost. Expression of CD44v6 was weak and restricted to some cells at the bottom of normal crypts. No obvious change was observed before appearance of severe dysplasia. In carcinomas, a strong but irregular expression of A, H and CD44v6 antigens was observed. In moderately differentiated carcinomas, A and H antigens were present at the apical surface of cells, whereas CD44v6 was found at the basolateral side. Only carcinomatous cells with loss of polarity, found in poorly differentiated cancers or infiltrated in the muscularis mucosae, were found to coexpress blood-group H or A and CD44v6 antigens at their surface.
Hallouin
F
F
INSERM U419, Institut de biologie, Nantes, France.
Goupille
C
C
le Cabellec
M
M
Bara
J
J
le Pendu
J
J
D000017:ABO Blood-Group System / Q000276:immunology*; D000230:Adenocarcinoma / Q000139:chemically induced / Q000276:immunology*; D000818:Animals; D003110:Colonic Neoplasms / Q000139:chemically induced / Q000276:immunology*; D004195:Disease Models, Animal; D000939:Epitopes / Q000276:immunology; D005260:Female; D018960:Hyaluronan Receptors / Q000378:metabolism*; D007150:Immunohistochemistry; D007413:Intestinal Mucosa / Q000276:immunology / Q000473:pathology; D011230:Precancerous Conditions / Q000276:immunology; D051381:Rats; D017208:Rats, Wistar
D000017:ABO Blood-Group System; D000939:Epitopes; D018960:Hyaluronan Receptors