Inhibitory effects of 12-O-tetradecanoylphorbol-13-acetate on dye leakage from single Novikoff cells and on dye transfer between reaggregated cell pairs.
The inhibitory effects of 12-O-tetradeconylphorbol-13-acetate (TPA) on the Lucifer Yellow leakage in single (nondissociated and dissociated) Novikoff cells in the presence of ethyleneglycotetraacetic acid (EGTA) and transfer between reaggregated cell pairs were investigated. Under treatment with TPA, single Novikoff cells showed inhibition of the dye leakage in the presence of EGTA, especially after 60-min treatment. There were only slight differences between nondissociated and dissociated cells in these experiments. The dye transfer in reaggregated cell pairs were significantly inhibited after 30-min treatment with TPA. In pretreatment with TPA during the recovery period of reaggregation the dye transfer in cell pairs was completely blocked. It was suggested that TPA blocks the assembly of gap junction, while it had no remarkable effect on gating mechanism of the hemichannels.
Liu
T F
TF
Laboratory of Biomembrane and Membrane Biotechnology, College of Life Sciences, Peking University, Beijing, China.
Paulson
A F
AF
Li
H Y
HY
Atkinson
M M
MM
Johnson
R G
RG
D000818:Animals; D002450:Cell Communication / Q000187:drug effects; D002463:Cell Membrane Permeability / Q000187:drug effects; D002469:Cell Separation; D002614:Chelating Agents; D004533:Egtazic Acid; D005456:Fluorescent Dyes / Q000737:chemistry* / Q000378:metabolism; D017629:Gap Junctions / Q000187:drug effects; D007478:Iontophoresis; D007546:Isoquinolines / Q000737:chemistry* / Q000378:metabolism; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology*; D014407:Tumor Cells, Cultured / Q000187:drug effects
D002614:Chelating Agents; D005456:Fluorescent Dyes; D007546:Isoquinolines; D004533:Egtazic Acid; C017475:lucifer yellow; D013755:Tetradecanoylphorbol Acetate