Site-specific modification of 4.5S RNA apical domain by complementary oligodeoxynucleotides carrying an alkylating group.
Site-specific alkylation of RNA by reactive oligodeoxynucleotides provides structural information and represents the first step towards the design of RNA derivatives to be used for functional studies. Specific alkylation of 4.5S RNA at G53, the first base of the apical tetraloop, was achieved by incubation with oligodeoxynucleotide ON2, complementary to nucleotides 38-53, which carries a p-(N-2-chloroethyl-N-methylamino)benzylamidophosphate group at the 5' end. Alkylation efficiency was increased by a factor of 6, without alteration of specificity, in the presence of a helper oligodeoxynucleotide, ON1, complementary to nucleotides 58-71 of the opposite strand of the RNA helix. A second reactive oligodeoxynucleotide, ON1-3'-R, was obtained by attaching the alkylating group to the 3' end of ON1. ON1-3'-R was able to modify G58. In the presence of ON2 as a helper oligodeoxynucleotide, the specificity of ON1-3'-R changes and efficient alkylation of nucleotides G54, A56 and G57 of the apical region of 4.5S RNA was observed.
Bulygin
K
K
Institute of Bioorganic Chemistry, Siberian Branch of Russian Academy of Sciences, Novosibirsk.
Malygin
A
A
Karpova
G
G
Westermann
P
P
D000477:Alkylating Agents / Q000737:chemistry*; D016297:Mutagenesis, Site-Directed; D009841:Oligonucleotides / Q000737:chemistry / Q000378:metabolism; D009843:Oligoribonucleotides / Q000737:chemistry; D012329:RNA, Bacterial / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism
D000477:Alkylating Agents; D009841:Oligonucleotides; D009843:Oligoribonucleotides; D012329:RNA, Bacterial