Hydrostatic pressure enhances vital staining with carboxyfluorescein or carboxydichlorofluorescein in Saccharomyces cerevisiae: efficient detection of labeled yeasts by flow cytometry.
The extent of intracellular accumulation of the fluorescent dye carboxyfluorescein or carboxydichlorofluorescein (CDCF) in Saccharomyces cerevisiae was found to be increased 5- to 10-fold under a nonlethal hydrostatic pressure of 30 to 50 MPa. This observation was confirmed by analysis of individual labeled cells by flow cytometry. The pressure-induced enhancement of staining with CDCF required D-glucose and was markedly inhibited by 2-deoxy-D-glucose, suggesting that glucose metabolism has a role in the process.
Abe
F
F
DEEPSTAR Group, Japan Marine Science and Technology Center, Yokosuka, Japan. abef@jamstec.go.jp
D005434:Flow Cytometry; D005452:Fluoresceins / Q000032:analysis*; D005947:Glucose / Q000378:metabolism; D006874:Hydrostatic Pressure; D012441:Saccharomyces cerevisiae / Q000737:chemistry*; D013194:Staining and Labeling
D005452:Fluoresceins; C053897:5(6)-carboxy-2',7'-dichlorofluorescein; C024098:6-carboxyfluorescein; D005947:Glucose