Purification of an extracellular signaling molecule involved in production of aerial mycelium by Streptomyces coelicolor.
We have extensively purified a factor from conditioned medium that restores aerial mycelium formation to a mutant of Streptomyces coelicolor that is defective in morphological differentiation. Response to this factor is shown to depend on the presence of the BldK oligopeptide import system. We suggest that this substance acts at the first step in a putative cascade of developmental regulatory signals.
Nodwell
J R
JR
Department of Molecular and Cellular Biology, Harvard University, Cambridge, Massachusetts 02138, USA.
Losick
R
R
D001692:Biological Transport; D017077:Culture Media, Conditioned; D005798:Genes, Bacterial; D009154:Mutation; D009842:Oligopeptides / Q000737:chemistry / Q000302:isolation & purification* / Q000378:metabolism; D015398:Signal Transduction; D013302:Streptomyces / Q000166:cytology / Q000235:genetics / Q000254:growth & development* / Q000378:metabolism
D017077:Culture Media, Conditioned; D009842:Oligopeptides