title: Post-transcriptional control of Pseudomonas aeruginosa lasB expression involves the 5' untranslated region of the mRNA.
abstract: To investigate the regulatory role of the 5' untranslated region of Pseudomonas aeruginosa lasB mRNA, several lasB-lacZ protein and operon fusions were generated. By examining expression of these fusions in strain PAO1 we showed that the 5' untranslated leader sequence was involved in the post-transcriptional iron regulation of elastase. Two components of the unusually long 139 nucleotide lasB 5' untranslated region may be involved in post-transcriptional control. The first 101 nucleotides of the lasB mRNA may contain a translational enhancer element and an element that enhances transcript accumulation. However, the iron responsive element in the lasB mRNA requires sequences downstream of nucleotide +104.
authors.affiliation: Department of Microbiology and Infectious Diseases, University of Calgary, Alta, Canada.
authors.forename: M J
authors.initials: MJ
authors.lastname: Brumlik
authors.forename: D G
authors.initials: DG
authors.lastname: Storey
mesh_terms: D001426:Bacterial Proteins; D015964:Gene Expression Regulation, Bacterial; D008666:Metalloendopeptidases / Q000235:genetics*; D011401:Promoter Regions, Genetic; D011550:Pseudomonas aeruginosa / Q000235:genetics*; D012333:RNA, Messenger / Q000235:genetics*; D014158:Transcription, Genetic
chemical_list: D001426:Bacterial Proteins; D012333:RNA, Messenger; D008666:Metalloendopeptidases; C043859:pseudolysin, Pseudomonas aeruginosa