title: Esculetin prevents liver damage induced by paracetamol and CCL4.
abstract: Esculetin, a phenolic compound found in Cichorium intybus and Bougainvllra spectabillis was investigated for its possible protective effect against paracetamol and CCl4-induced hepatic damage. Paracetamol produced 100% mortality at the dose of 1 g kg-1 in mice while pre-treatment of animals with esculetin (6 mg kg-1) reduced the death rate to 40%. Oral administration of paracetamol (640 mg kg-1) produced liver damage in rats as manifested by the rise in serum enzyme levels of alkaline phosphatase (ALP) and aminotransferases (AST and ALT). Pre-treatment of rats with esculetin (6 mg kg-1) prevented the paracetamol-induced rise in serum enzymes. The hepatotoxic dose of CCl4 (1.5 ml kg-1; orally) also raised serum ALP, AST and ALT levels. The same dose of esculetin (6 mg kg-1) was able to prevent the CCl4-induced rise in serum enzymes. Esculetin also prevented CCl4-induced prolongation in pentobarbital sleeping time confirming hepatoprotectivity. These results indicate that esculetin possesses anti-hepatotoxic activity and the presence of this compound in Cichorium intybus and Bougainvllra spectabillis may explain the folkloric use of these plants in liver damage.
authors.affiliation: Department of Physiology and Pharmacology, Aga Khan University Medical College, Karachi, Pakistan.
authors.forename: A H
authors.initials: AH
authors.lastname: Gilani
authors.forename: K H
authors.initials: KH
authors.lastname: Janbaz
authors.forename: B H
authors.initials: BH
authors.lastname: Shah
mesh_terms: D000082:Acetaminophen; D000818:Animals; D000975:Antioxidants / Q000627:therapeutic use*; D002251:Carbon Tetrachloride; D056486:Chemical and Drug Induced Liver Injury; D008107:Liver Diseases / Q000517:prevention & control*; D008297:Male; D051379:Mice; D051381:Rats; D017208:Rats, Wistar; D012890:Sleep / Q000187:drug effects; D014468:Umbelliferones / Q000627:therapeutic use*
chemical_list: D000975:Antioxidants; D014468:Umbelliferones; D000082:Acetaminophen; D002251:Carbon Tetrachloride; C007628:esculetin