Molecular interaction between proteins involved in EvgAS signal transduction of Escherichia coli.
EvgA and EvgS constitute one two-component signal transduction system in Escherichia coli. Although probable signaling domains of these proteins have been estimated, the molecular mechanism of their interaction remains to be elucidated. Here, we investigated protein to protein interactions between EvgA and EvgS and also between the EvgAS system and other related signaling pathways by means of surface plasmon resonance. EvgA and EvgS interacted directly and inhibition of phosphorylation of their functional domains abolished formation of the EvgAS complex. No interaction was observed either between EvgA and Bordetella BvgS or BvgA and EvgS. OmpR, a response regulator for the osmoregulative gene expression of E. coli, had similar but not identical behavior towards EvgS to that of EvgA. These results indicate that interaction between the signaling proteins is closely related to phosphorylation of the functional domain of the proteins.
Tanabe
H
H
Department of Agricultural Chemistry, Kinki University, Nara, Japan.
Masuda
T
T
Yamasaki
K
K
Katoh
A
A
Yoshioka
S
S
Utsumi
R
R
D001425:Bacterial Outer Membrane Proteins / Q000235:genetics / Q000378:metabolism; D001426:Bacterial Proteins / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D004926:Escherichia coli / Q000378:metabolism*; D029968:Escherichia coli Proteins; D009097:Multienzyme Complexes; D010766:Phosphorylation; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism; D015398:Signal Transduction; D014157:Transcription Factors / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*
D001425:Bacterial Outer Membrane Proteins; D001426:Bacterial Proteins; C065601:BvgA protein, Bacteria; D029968:Escherichia coli Proteins; C489314:EvgA protein, E coli; D009097:Multienzyme Complexes; D011993:Recombinant Fusion Proteins; D014157:Transcription Factors; C080662:bvgS protein, Bordetella pertussis; C057843:envZ protein, E coli