title: Influence of temperature and activity on the metabolic rate of adult Drosophila melanogaster.
abstract: We measured metabolic rates of adult male Drosophila melanogaster allowed to evolve in the laboratory at 18 and 25 degrees C and compared these with measurements of metabolic rates of flies collected along a latitudinal gradient in Australia. Metabolic rates of flies that had evolved in the laboratory at low temperature were 5-7% higher than those of flies allowed to evolve at high temperature. Metabolic rates of field collected increased with latitude when measured at 18 degrees C but not at higher temperature (25 degrees C) and were about 9% greater in high latitude (approximately 41'00) flies than low latitude (16'53) flies. Metabolic rate was strongly influenced by measurement temperature; estimated Q10s ranged from 1.79 to 2.5 for measurements made at 18 and 25 degrees C. Metabolic rate scaled isometrically with body mass; the estimated slope of a ln-ln regression of metabolic rate and body mass was 1.03 +/- 0.1. We used our measures of metabolic rate and activity to estimate the minimum cost of transport (MCOT) while walking. The estimates of MCOT have high standard errors (lab, 34.30 +/- 14.2 ml O2/g/km; and field, 38.0 +/- 17.0 ml O2/g/km); however, they differ by only 3-9% from predicted values based on allometric relationships reported in the literature.
authors.affiliation: Department of Zoology NJ-15, University of Washington, Seattle 98195, USA. berrigan@zoology.washington.edu
authors.forename: D
authors.initials: D
authors.lastname: Berrigan
authors.forename: L
authors.initials: L
authors.lastname: Partridge
mesh_terms: D000818:Animals; D001315:Australia; D001481:Basal Metabolism / Q000502:physiology*; D002245:Carbon Dioxide / Q000378:metabolism; D004331:Drosophila melanogaster / Q000502:physiology*; D004734:Energy Metabolism / Q000502:physiology; D004777:Environment; D008297:Male; D009043:Motor Activity / Q000502:physiology*; D010101:Oxygen Consumption / Q000502:physiology; D013147:Spirometry; D013696:Temperature; D016138:Walking
chemical_list: D002245:Carbon Dioxide