title: Internalization of Aeromonas hydrophila by fish epithelial cells can be inhibited with a tyrosine kinase inhibitor.
abstract: Aeromonas hydrophila is a Gram-negative bacterium that is pathogenic in fish, causing motile aeromonad septicaemia. It can enter (invade) fish cells, and survive as an intracellular parasite. The host-pathogen interaction and signal transduction pathway were studied by screening signal transduction inhibitors using carp epithelial cells and a virulent strain of the bacterium, PPD134/91. Genistein, a tyrosine kinase inhibitor, postponed internalization of A. hydrophila into host cells, suggesting that tyrosine phosphorylation plays a role in internalization. In contrast, staurosporine, a protein kinase C inhibitor, and sodium orthovanadate, a protein tyrosine phosphatase inhibitor, accelerated internalization of PPD134/91. Other virulent strains of A. hydrophila were also examined and it is likely that all strains, irrespective of serogroup, use the same signalling pathway to facilitate bacterial uptake.
authors.affiliation: School of Biological Sciences, Faculty of Science, National University of Singapore, 10 Kent Ridge Crescent, Singapore 119260.
authors.forename: E
authors.initials: E
authors.lastname: Tan
authors.affiliation: School of Biological Sciences, Faculty of Science, National University of Singapore, 10 Kent Ridge Crescent, Singapore 119260.
authors.forename: K W
authors.initials: KW
authors.lastname: Low
authors.affiliation: Department of Pharmacology, Faculty of Medicine, National University of Singapore, 10 Kent Ridge Crescent, Singapore 119260.
authors.forename: W S F
authors.initials: WSF
authors.lastname: Wong
authors.affiliation: School of Biological Sciences, Faculty of Science, National University of Singapore, 10 Kent Ridge Crescent, Singapore 119260.
authors.forename: K Y
authors.initials: KY
authors.lastname: Leung
mesh_terms: D000199:Actins / Q000378:metabolism; D016980:Aeromonas hydrophila; D000818:Animals; D002478:Cells, Cultured; D004791:Enzyme Inhibitors / Q000494:pharmacology*; D004847:Epithelial Cells / Q000382:microbiology; D005399:Fishes; D019833:Genistein / Q000494:pharmacology*; D016905:Gram-Negative Bacterial Infections / Q000382:microbiology*; D006790:Host-Parasite Interactions; D018613:Microscopy, Confocal; D011493:Protein Kinase C / Q000037:antagonists & inhibitors / Q000378:metabolism; D017027:Protein Tyrosine Phosphatases / Q000037:antagonists & inhibitors / Q000378:metabolism; D011505:Protein-Tyrosine Kinases / Q000037:antagonists & inhibitors* / Q000378:metabolism; D015398:Signal Transduction; D019311:Staurosporine / Q000494:pharmacology; D014638:Vanadates / Q000494:pharmacology
chemical_list: D000199:Actins; D004791:Enzyme Inhibitors; D014638:Vanadates; D019833:Genistein; D011505:Protein-Tyrosine Kinases; D011493:Protein Kinase C; D017027:Protein Tyrosine Phosphatases; D019311:Staurosporine