MAGE-11 protein is highly conserved in higher organisms and located predominantly in the nucleus.
The MAGE-11 gene belongs to a family whose products were identified first in tumor tissue. The MAGE-11 gene product has not been characterized in detail. We have isolated MAGE-11 cDNA from HeLa cells and confirmed the presence of MAGE-11 protein and of at least 2 other MAGE proteins (MAGE-1 and MAGE-3) in this cell line. Monoclonal antibodies (MAbs), obtained by using a GST-MAGE-11 fusion protein, detect MAGE-11 protein in HeLa cells as a 48 kDa protein. In contrast to other known proteins of the MAGE family, MAGE-11 is found mainly in the nucleus. Immunoprecipitation out of whole-cell extracts from different species reveals that MAGE-11 protein is highly conserved among mammalian cells, suggesting a conserved and important function.
Jurk
M
M
Institut für Biochemie der Ludwig-Maximilians-Universität, Munich, Germany.
Kremmer
E
E
Schwarz
U
U
Förster
R
R
Winnacker
E L
EL
D000818:Animals; D000911:Antibodies, Monoclonal; D000951:Antigens, Neoplasm; D006801:Humans; D058950:Melanoma-Specific Antigens; D018613:Microscopy, Confocal; D008970:Molecular Weight; D009363:Neoplasm Proteins / Q000378:metabolism*; D009687:Nuclear Proteins / Q000276:immunology / Q000378:metabolism*; D011233:Precipitin Tests; D012333:RNA, Messenger / Q000235:genetics; D051381:Rats; D013045:Species Specificity
D000911:Antibodies, Monoclonal; D000951:Antigens, Neoplasm; C072011:MAGEA1 protein, human; C110882:MAGEA11 protein, human; C072013:MAGEA3 protein, human; D058950:Melanoma-Specific Antigens; D009363:Neoplasm Proteins; D009687:Nuclear Proteins; D012333:RNA, Messenger