title: A surface plasmon resonance assay for the binding of Amaranthus hypochondriacus var. Mexico lectin to glycoprotein.
abstract: We have used the analytical system based on surface plasmon resonance to monitor the interaction between Amaranthus hypochondriacus var. Mexico lectin and four different fetuins; fetuin, asialofetuin, agalactofetuin, and agalactosaminofetuin. Agalactofetuin and agalactosaminofetuin were prepared by enzymic digestion of asialofetuin using jack bean beta-galactosidase or endo-alpha-N-acetylgalactosaminidase from Diplococcus pneumoniae. Ligands were immobilized onto a sensor surface via amide linkages. The lectin interacted most strongly with asialofetuin, but not with agalactosaminofetuin. The binding of the lectin to asialofetuin was inhibited by N-acetylgalactosamine or Gal beta 1-->3GalNAc in a dose-dependent manner.
authors.affiliation: Department of Agricultural Chemistry, Faculty of Bioresources, Mie University, Japan.
authors.forename: M
authors.initials: M
authors.lastname: Ozeki
authors.forename: Y
authors.initials: Y
authors.lastname: Furuichi
authors.forename: H
authors.initials: H
authors.lastname: Umekawa
authors.forename: T
authors.initials: T
authors.lastname: Takahashi
mesh_terms: D001212:Asialoglycoproteins / Q000378:metabolism; D001667:Binding, Competitive / Q000187:drug effects; D015374:Biosensing Techniques; D060748:Fetuins; D006023:Glycoproteins / Q000378:metabolism*; D037102:Lectins / Q000378:metabolism*; D010940:Plant Proteins / Q000378:metabolism*; D011485:Protein Binding / Q000187:drug effects; D000509:alpha-Fetoproteins / Q000378:metabolism*
chemical_list: D001212:Asialoglycoproteins; D060748:Fetuins; D006023:Glycoproteins; D037102:Lectins; D010940:Plant Proteins; D000509:alpha-Fetoproteins; C017029:asialofetuin