title: Cellular arrangement of human breast cancer cell lines determines hemostatic parameters.
abstract: Two in vitro models are compared to investigate whether cellular configuration or composition of the matrix in which the cells are cultured influences growth and/or prognostic parameters. T47D, MCF-7 and Hs578T breast cancer cell lines were cultured on two different matrices (agarose and collagen). Growth curves, biological markers (Ki-67, p53 and bcl-2) and the expression of hemostatic parameters were studied. The tested hemostatic parameters were urokinase-type plasminogen activator, tissue-type plasminogen activator and plasminogen activator inhibitor as fibrinolytic parameters and von Willbrand factor, tissue factor, antithrombin III, factor X and factor Xa as coagulation parameters. We found that T47D and MCF-7 formed spheroids in both matrices. Hs578T did not form spheroids; instead, the cells remained single cells in the agarose matrix and grew invasively through the collagen matrix. Expression of the biological markers was similar for spheroids and monolayers. In contrast, a clear difference in expression of hemostatic factors by spheroids and monolayers was found.
authors.affiliation: Department of Internal Medicine, University Hospital Maastricht, The Netherlands. a.griffioen@intmed.unimaas.nl
authors.forename: A F
authors.initials: AF
authors.lastname: Barendsz-Janson
authors.forename: A D
authors.initials: AD
authors.lastname: Muller
authors.forename: H H
authors.initials: HH
authors.lastname: Lichtenbeld
authors.forename: M C
authors.initials: MC
authors.lastname: Van Dam-Mieras
authors.forename: H F
authors.initials: HF
authors.lastname: Hillen
mesh_terms: D015415:Biomarkers; D001779:Blood Coagulation Factors / Q000096:biosynthesis; D001943:Breast Neoplasms / Q000473:pathology*; D002455:Cell Division; D002478:Cells, Cultured; D003094:Collagen; D005260:Female; D006487:Hemostasis; D006801:Humans; D019394:Ki-67 Antigen / Q000032:analysis / Q000096:biosynthesis; D007700:Kinetics; D015849:Plasminogen Inactivators / Q000096:biosynthesis; D011379:Prognosis; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000032:analysis / Q000096:biosynthesis; D012685:Sepharose; D010959:Tissue Plasminogen Activator / Q000096:biosynthesis; D016159:Tumor Suppressor Protein p53 / Q000032:analysis / Q000096:biosynthesis; D014568:Urokinase-Type Plasminogen Activator / Q000096:biosynthesis
chemical_list: D015415:Biomarkers; D001779:Blood Coagulation Factors; D019394:Ki-67 Antigen; D015849:Plasminogen Inactivators; D019253:Proto-Oncogene Proteins c-bcl-2; D016159:Tumor Suppressor Protein p53; D003094:Collagen; D012685:Sepharose; D010959:Tissue Plasminogen Activator; D014568:Urokinase-Type Plasminogen Activator