title: Influence of both TCR repertoire and severity of the atopic status on the cytokine secretion profile of Parietaria officinalis-specific T cells.
abstract: Peripheral blood mononuclear cells (PBMC) from both nonatopic and Parietaria officinalis-sensitive donors proliferated in response to the allergen Par o 1 and developed into Par o 1-specific T cell lines and clones, which also showed reactivity for Par o 1-derived peptides. Virtually all Par o 1-specific T cell lines and large numbers of Par o 1-specific T cell clones proliferated in response to two Par o 1 nonapeptides (p92 and p96), which probably contain immunodominant epitopes of the Par o 1 allergen. Both p92- and p96-specific T cell clones showed the ability to produce IFN-gamma, but p92-specific T cell clones produced significantly lower amounts of IL-4 and IL-5 than p96-specific T cell clones, indicating that distinct epitopes, able to elicit functionally different T helper cell responses, may coexist in Par o 1. However, p92-specific T cell clones derived from atopic subjects with high IgE serum levels (high IgE producers) secreted significantly higher amounts of IL-4 and IL-5 than corresponding T cell clones generated from nonatopic subjects or patients with low IgE serum levels (low IgE producers), whereas p96-specific T cell clones secreted high IL-4 and IL-5 concentrations irrespective of whether they derived from high or low IgE producers. The addition of IL-4 and anti-IL-12 mAb to bulk culture significantly up-regulated the development of p92-specific T cells into IL-4-producing cells, whereas the addition of IL-12 and anti-IL-4 mAb shifted the differentiation of p96-specific T cells towards IFN-gamma-producing cells. Taken together, these results suggest that the cytokine profile of allergen-specific T cells is influenced by both the T cell receptor repertoire and the severity of atopic status and can be modulated, at least in vitro, by stimulation with the specific peptide in the presence, or after removal, of appropriate cytokines.
authors.affiliation: Istituto di Medicina Interna e Immunoallergologia, University of Florence, Italy.
authors.forename: P
authors.initials: P
authors.lastname: Parronchi
authors.forename: S
authors.initials: S
authors.lastname: Sampognaro
authors.forename: F
authors.initials: F
authors.lastname: Annunziato
authors.forename: F
authors.initials: F
authors.lastname: Brugnolo
authors.forename: A
authors.initials: A
authors.lastname: Radbruch
authors.forename: F
authors.initials: F
authors.lastname: Di Modugno
authors.forename: A
authors.initials: A
authors.lastname: Ruffilli
authors.forename: S
authors.initials: S
authors.lastname: Romagnani
authors.forename: E
authors.initials: E
authors.lastname: Maggi
mesh_terms: D000485:Allergens / Q000276:immunology*; D002999:Clone Cells / Q000378:metabolism; D015329:Gene Rearrangement, T-Lymphocyte; D006801:Humans; D007371:Interferon-gamma / Q000378:metabolism; D018664:Interleukin-12 / Q000494:pharmacology; D015847:Interleukin-4 / Q000378:metabolism / Q000494:pharmacology; D015848:Interleukin-5 / Q000378:metabolism; D008213:Lymphocyte Activation; D008222:Lymphokines / Q000378:metabolism*; D010940:Plant Proteins / Q000276:immunology*; D011058:Pollen / Q000276:immunology*; D011948:Receptors, Antigen, T-Cell / Q000235:genetics*; D012221:Rhinitis, Allergic, Perennial / Q000209:etiology / Q000276:immunology*; D012720:Severity of Illness Index; D013601:T-Lymphocytes / Q000276:immunology* / Q000378:metabolism; D018417:Th1 Cells / Q000378:metabolism; D018418:Th2 Cells / Q000378:metabolism
chemical_list: D000485:Allergens; D015848:Interleukin-5; D008222:Lymphokines; D010940:Plant Proteins; D011948:Receptors, Antigen, T-Cell; D018664:Interleukin-12; D015847:Interleukin-4; D007371:Interferon-gamma