title: Sensitive high-performance liquid chromatographic method with fluorometric detection for the simultaneous determination of gabapentin and vigabatrin in serum and urine.
abstract: Serum concentrations of the antiepileptic drug gabapentin (GBP) are usually determined by high-performance liquid chromatography (HPLC) using UV photometric detection after pre-column derivatization with 2,4,6-trinitrobenzenesulphonic acid. Vigabatrin levels in serum are determined by HPLC using fluorescence detection. Like vigabatrin (VGB), gabapentin has also a primary amine group that easily reacts with o-phthaldialdehyde reagent and produces a fluorescing substance. By the use of fluorometric detection, GBP can be determined more simply, sensitively and simultaneously with VGB. The day-to-day coefficient of variation for the determination of GBP in a pooled serum was 4.0% (n=17; serum concentration, 13.8 micromol/l) and forVGB was 3.1% (n=21; serum concentration, 26.4 micromol/l). The lower limit of detection is 0.5 micromol/l for both drugs and the method is linear up to 500 micromol/l for GBP and 1300 micromol/l for VGB.
authors.affiliation: Swiss Epilepsy Centre, Zürich.
authors.forename: N
authors.initials: N
authors.lastname: Wad
authors.forename: G
authors.initials: G
authors.lastname: Krämer
mesh_terms: D000085:Acetates / Q000097:blood* / Q000652:urine*; D000588:Amines; D000927:Anticonvulsants / Q000097:blood* / Q000652:urine*; D002851:Chromatography, High Pressure Liquid; D003509:Cyclohexanecarboxylic Acids; D000077206:Gabapentin; D006801:Humans; D012680:Sensitivity and Specificity; D013050:Spectrometry, Fluorescence; D020888:Vigabatrin; D005680:gamma-Aminobutyric Acid / Q000031:analogs & derivatives* / Q000097:blood / Q000652:urine
chemical_list: D000085:Acetates; D000588:Amines; D000927:Anticonvulsants; D003509:Cyclohexanecarboxylic Acids; D005680:gamma-Aminobutyric Acid; D000077206:Gabapentin; D020888:Vigabatrin