Latex immunoassay for rapid detection of Newcastle disease virus.
A rapid test has been developed based on the technique of latex immunoassay for the detection of Newcastle disease virus from suspected tissue suspensions. The latex particles were sensitised with globulins and were used for antigen detection. Of the 258 samples tested, 165 samples were positive by this kit which was compared for its efficacy with the standard OIE approved haemagglutination (HA) and haemagglutination inhibition (HI) tests. No significant difference (P > 0.05) was observed between the tests. The sensitivity and specificity of the developed test was 94.19% and 87.63% respectively.
Thirumurugan
G
G
Department of Animal Biotechnology, Madras Veterinary College, Tamil Nadu Veterinary and Animal Sciences University, India.
Jayakumar
R
R
Kumanan
K
K
Venugopalan
A T
AT
Nachimuthu
K
K
D000818:Animals; D001921:Brain / Q000821:virology; D002645:Chickens; D004196:Disease Outbreaks / Q000662:veterinary; D005766:Gastrointestinal Contents / Q000382:microbiology; D006386:Hemagglutination Tests; D007080:Ileocecal Valve / Q000821:virology; D007118:Immunoassay / Q000379:methods; D007841:Latex Fixation Tests / Q000662:veterinary; D009521:Newcastle Disease / Q000175:diagnosis* / Q000453:epidemiology; D009522:Newcastle disease virus / Q000302:isolation & purification*; D013154:Spleen / Q000821:virology; D014132:Trachea / Q000821:virology