title: Natural ligand of mouse CD1d1: cellular glycosylphosphatidylinositol.
abstract: Mouse CD1d1, a member of the CD1 family of evolutionarily conserved major histocompatibility antigen-like molecules, controls the differentiation and function of a T lymphocyte subset, NK1+ natural T cells, proposed to regulate immune responses. The CD1d1 crystal structure revealed a large hydrophobic binding site occupied by a ligand of unknown chemical nature. Mass spectrometry and metabolic radiolabeling were used to identify cellular glycosylphosphatidylinositol as a major natural ligand of CD1d1. CD1d1 bound glycosylphosphatidylinositol through its phosphatidylinositol aspect with high affinity. Glycosylphosphatidylinositol or another glycolipid could be a candidate natural ligand for CD1d1-restricted T cells.
authors.affiliation: Department of Microbiology and Immunology, The Pennsylvania State University College of Medicine, Milton S. Hershey Medical Center, Hershey, PA 17033-0850, USA. sjoyce@bcmic.hmc.psu.edu
authors.forename: S
authors.initials: S
authors.lastname: Joyce
authors.forename: A S
authors.initials: AS
authors.lastname: Woods
authors.forename: J W
authors.initials: JW
authors.lastname: Yewdell
authors.forename: J R
authors.initials: JR
authors.lastname: Bennink
authors.forename: A D
authors.initials: AD
authors.lastname: De Silva
authors.forename: A
authors.initials: A
authors.lastname: Boesteanu
authors.forename: S P
authors.initials: SP
authors.lastname: Balk
authors.forename: R J
authors.initials: RJ
authors.lastname: Cotter
authors.forename: R R
authors.initials: RR
authors.lastname: Brutkiewicz
mesh_terms: D000818:Animals; D018949:Antigens, CD1 / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism*; D001665:Binding Sites; D017261:Glycosylphosphatidylinositols / Q000737:chemistry / Q000378:metabolism*; D008024:Ligands; D013058:Mass Spectrometry; D051379:Mice; D012995:Solubility; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D016176:T-Lymphocyte Subsets / Q000276:immunology
chemical_list: D018949:Antigens, CD1; D017261:Glycosylphosphatidylinositols; D008024:Ligands