title: Genetic diversity in porcine Oesophagostomum dentatum and O. quadrispinulatum and their delineation by isoenzyme analysis.
abstract: The genetic diversity in eight strains of Oesophagostomum dentatum and O. quadrispinulatum was investigated by the electrophoresis study of ten enzyme systems. The loci Idh-2, Fbp, Sdh, and Pgm were found to be diagnostic between the species examined. Both the proportion of fixed allelic differences (26.3%) and the genetic distance coefficient (D = 0.54) are well above the range for differentiation of valid species. Isoenzyme patterns of susceptible and resistant lines of O. dentatum showed at polymorphic loci a reduced genetic heterogeneity in the latter group. No qualitative difference in terms of the presence/absence of alleles was observed among susceptible and resistant isolates with the enzymes studied. The detection of one possible hybrid indicates that introgression in O. dentatum and O. quadrispinuatum may occur.
authors.affiliation: Danish Center for Experimental Parasitology, Royal Veterinary and Agricultural University, Frederiksberg C, Denmark. snabel@saske.sk
authors.forename: V
authors.initials: V
authors.lastname: Snábel
authors.forename: M
authors.initials: M
authors.lastname: Várady
authors.forename: C M
authors.initials: CM
authors.lastname: Christensen
authors.forename: H
authors.initials: H
authors.lastname: Bjørn
authors.forename: P
authors.initials: P
authors.lastname: Nansen
authors.forename: J
authors.initials: J
authors.lastname: Corba
authors.forename: P
authors.initials: P
authors.lastname: Dubinský
mesh_terms: D000483:Alleles; D000818:Animals; D000969:Antinematodal Agents / Q000494:pharmacology; D004351:Drug Resistance / Q000235:genetics; D005260:Female; D017238:Genes, Helminth; D005819:Genetic Markers; D014644:Genetic Variation; D007525:Isoelectric Focusing; D007527:Isoenzymes / Q000032:analysis / Q000235:genetics*; D008297:Male; D009814:Oesophagostomiasis / Q000469:parasitology / Q000662:veterinary; D009815:Oesophagostomum / Q000145:classification / Q000187:drug effects / Q000201:enzymology / Q000235:genetics*; D013045:Species Specificity; D013552:Swine; D013553:Swine Diseases / Q000469:parasitology
chemical_list: D000969:Antinematodal Agents; D005819:Genetic Markers; D007527:Isoenzymes