Isolation of ergosterol peroxide and its reversion to ergosterol in the pathogenic fungus Sporothrix schenckii.
Ergosterol peroxide, a presumed product of the H2O2-dependent enzymatic oxidation of ergosterol, has been isolated from yeast forms of the pathogenic fungus Sporothrix schenckii. The substance, which may have a role in fungal virulence, has been characterized mainly using spectroscopic methods (1H and 13C nuclear magnetic resonance and high resolution mass spectra). The purified compound showed a molecular formula of C28H44O3, displaying characteristic features of epidioxy sterols and was reverted to ergosterol when submitted to S. schenckii enzymatic extract.
Sgarbi
D B
DB
Departamento de Microbiologia e Parasitologia, Universidade Federal Fluminense, Niterói, RJ.
da Silva
A J
AJ
Carlos
I Z
IZ
Silva
C L
CL
Angluster
J
J
Alviano
C S
CS
D000970:Antineoplastic Agents / Q000302:isolation & purification / Q000378:metabolism*; D002855:Chromatography, Thin Layer; D004875:Ergosterol / Q000031:analogs & derivatives* / Q000302:isolation & purification / Q000378:metabolism*; D009682:Magnetic Resonance Spectroscopy; D013175:Sporothrix / Q000737:chemistry*
D000970:Antineoplastic Agents; C036071:ergosterol-5,8-peroxide; D004875:Ergosterol