On-line analysis by capillary separations interfaced to an ion trap storage/reflectron time-of-flight mass spectrometer.
The interface of high-resolution capillary separation methods to time-of-flight mass spectrometry (TOF-MS) has generated considerable interest since TOF can provide the rapid and sensitive detection required by high resolution separations. In recent years, our laboratory has developed a variety of high-resolution capillary separation methods interfaced to TOF-MS via an ion trap storage/reflectron time-of-flight (IT/reTOF) instrument. Using this hybrid configuration, detection of fast separations at very low detection levels has been successfully performed for on-line separations of peptides and protein digests using electrospray ionization. In this report, we review the current status in our laboratory of interfacing high-performance liquid chromatography, capillary electrophoresis, and capillary electrochromatography to an IT/reTOF-MS instrument and various applications that have been developed involving this technology.
Wu
J T
JT
Department of Chemistry, University of Michigan, Ann Arbor 48109-1055, USA.
Qian
M G
MG
Li
M X
MX
Zheng
K
K
Huang
P
P
Lubman
D M
DM
D002851:Chromatography, High Pressure Liquid; D019075:Electrophoresis, Capillary / Q000295:instrumentation / Q000379:methods*; D013058:Mass Spectrometry; D009862:Online Systems