Placental alkaline phosphatase in patients with seminoma measured by ELISA as compared to RIA.
Placental alkaline phosphatase (PLAP) has been shown to be a reliable tumor marker in the management of patients with seminoma. Radioimmunoassay (RIA) and Enzyme linked immunoassay (ELISA) procedures were compared for PLAP levels in serum of seminoma patients. The statistical analysis showed excellent correlationship between the two. It is evident from the results that performance of ELISA in terms of sensitivity specificity, precision, accuracy and reproducibility is equivalent to or better than RIA.
Redkar
S L
SL
Department of Biochemistry, Tata Memorial Hospital, Parel, Mumbai, India.
Damle
S R
SR
D000469:Alkaline Phosphatase / Q000032:analysis*; D014408:Biomarkers, Tumor / Q000097:blood*; D016022:Case-Control Studies; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006801:Humans; D008297:Male; D010920:Placenta / Q000201:enzymology*; D011247:Pregnancy; D011863:Radioimmunoassay; D018239:Seminoma / Q000201:enzymology*; D013736:Testicular Neoplasms / Q000201:enzymology*
D014408:Biomarkers, Tumor; D000469:Alkaline Phosphatase