[Detection of mycobacterium tuberculosis DNA using nested polymerase chain reaction in lymph nodes with sarcoidosis, fixed in formalin and embedded in paraffin].
An involvement of mycobacteria in the pathogenesis of sarcoidosis has often been hypothesized, but not confirmed reproducibly. In this study we applied a nested Polymerase Chain Reaction (PCR) to the insertion sequence IS6110 for detection of Mycobacterium tuberculosis (MT) DNA in formalin-fixed and paraffin-embedded tissues from patients with sarcoidosis, with tuberculosis and atypical mycobacteriosis confirmed by culture, and from negative control samples. MT-DNA could be detected in 2/30 samples of sarcoidosis, in 10/10 tuberculoses, in 0/5 atypical mycobacterioses and in 0/10 negative controls. Nested PCR confirmed its high sensitivity and specificity in detecting MT-DNA on archival histopathological specimens. From our results we conclude that in the granulomatous lesions of sarcoidosis MT-DNA is only sporadically demonstrable and probably it doesn't play a role in the pathogenesis of this disease.
Cannone
M
M
Istituto di Anatomia e Istologia Patologica, Università di Milano, Ospedale Luigi Sacco.
Vago
L
L
Porini
G
G
Bonetto
S
S
Cassi
C
C
Bramerio
M
M
Rizzato
G
G
Barberis
M C
MC
D004269:DNA, Bacterial / Q000032:analysis*; D005404:Fixatives; D005557:Formaldehyde; D006801:Humans; D008198:Lymph Nodes / Q000382:microbiology* / Q000473:pathology; D015270:Mycobacterium avium-intracellulare Infection / Q000150:complications / Q000382:microbiology / Q000473:pathology; D009169:Mycobacterium tuberculosis / Q000235:genetics / Q000302:isolation & purification*; D016612:Paraffin Embedding; D016133:Polymerase Chain Reaction; D012507:Sarcoidosis / Q000150:complications / Q000382:microbiology* / Q000473:pathology; D012680:Sensitivity and Specificity; D016707:Tissue Fixation; D014388:Tuberculosis, Lymph Node / Q000150:complications / Q000382:microbiology / Q000473:pathology
D004269:DNA, Bacterial; D005404:Fixatives; D005557:Formaldehyde
Ricerca del DNA del Mycobacterium tuberculosis mediante nested Polymerase Chain Reaction in linfonodi con sarcoidosi fissati in formalina e inclusi in paraffina.