Cell membrane lipid molecular dynamics in a solenoid versus a magnetically shielded room.
The generalized polarization function of the fluorescent probe 2-dimethylamino-6-lauroylnaphthalene has been used to evaluate the lipid dynamics in Friend erythroleukemia cell membrane. The values of this function varied during the culture growth cycle, showing decreased lipid dynamics 24-48 h from the cell seeding. When the cycle occurred in a solenoid producing a magnetic field of 70 microT at 50 Hz in addition to the 45 microT DC of the earth (short-term 4-day exposure), the membrane lipid dynamics during this same time-period decreased by about 10% (P < .04). After long-term (184 days) or extremely long-term (395 days) exposure of the cells to the magnetic field, little additional variation in the membrane lipid dynamics was observed, suggesting an adaptation phenomenon. A variation of membrane lipid dynamics was also observed due to in vitro cell differentiation (P < .02). Nevertheless, the exposure of both undifferentiating and differentiating cells to a highly attenuated magnetic field in a magnetically shielded room (20 nT DC plus 2.5 pT AC) did not induce any modification of membrane lipid dynamics.
Volpe
P
P
Department of Biology of the University of Rome Tor Vergata, Italy.
Parasassi
T
T
Esposito
C
C
Ravagnan
G
G
Giusti
A M
AM
Pasquarelli
A
A
Eremenko
T
T
D000818:Animals; D002453:Cell Cycle / Q000528:radiation effects; D002454:Cell Differentiation / Q000528:radiation effects; D002455:Cell Division / Q000528:radiation effects; D002462:Cell Membrane / Q000378:metabolism / Q000528:radiation effects; D004553:Electric Conductivity; D004574:Electromagnetic Fields / Q000009:adverse effects; D004780:Environment, Controlled; D004915:Leukemia, Erythroblastic, Acute / Q000378:metabolism / Q000473:pathology; D008563:Membrane Lipids / Q000378:metabolism* / Q000528:radiation effects; D051379:Mice; D013997:Time Factors; D014407:Tumor Cells, Cultured
D008563:Membrane Lipids