title: Preparation of PCR products for DNA sequencing.
abstract: We demonstrate that routine PCR product analytical agarose gels can also serve as preparative gels for quick DNA template purification before sequencing. The band of interest is excised, placed into a Gel Nebulizer inside a Micropure separator and rapidly purified in a single centrifugation step. Gel-purified PCR product, suitable for manual and automated sequencing, is delivered within 10 min.
authors.affiliation: Millipore, Danvers, MA, USA.
authors.forename: J T
authors.initials: JT
authors.lastname: Leonard
authors.forename: M B
authors.initials: MB
authors.lastname: Grace
authors.forename: G S
authors.initials: GS
authors.lastname: Buzard
authors.forename: M J
authors.initials: MJ
authors.lastname: Mullen
authors.forename: C B
authors.initials: CB
authors.lastname: Barbagallo
mesh_terms: D002498:Centrifugation / Q000379:methods; D004247:DNA / Q000302:isolation & purification*; D003956:Dialysis / Q000379:methods; D004587:Electrophoresis, Agar Gel; D016133:Polymerase Chain Reaction / Q000379:methods*; D017422:Sequence Analysis, DNA / Q000379:methods*
chemical_list: D004247:DNA