Kinetics of hematopoietic progenitor cell release induced by G-CSF-alone in children with solid tumors and leukemias.
The kinetics of peripheral blood progenitor cell (PBPC) release induced by G-CSF-alone at 10 microg/kg/day were monitored daily in 42 children with solid tumors and leukemias. Median 16- and 27-fold enrichment of circulating CD34+ cells and granulocyte-macrophage colony-forming units (CFU-GM) was noted with peak values occurring after the 4th or the 5th G-CSF dose. Individual values of PBCD34+ cell levels in patients with solid tumors were not significantly different after the 4th and after the 5th dose. The day-of-collection PBCD34+ cell concentration was related to the harvested CD34+ cell (P = 0.0001) and CFU-GM numbers (P = 0.0001). No correlations were found between PBPC enrichment and either patient age, body weight, diagnosis or pre-mobilization treatment duration. The median numbers of 1.1 x 10(6) CD34+ cells/kg and 28.1 x 10(4) CFU-GM/kg were derived from one patient's blood volume processed. Nineteen patients received G-CSF-alone primed grafts and had successful engraftment. Our data indicate that in 88% of children a single standard leukapheresis is sufficient to obtain a minimum graft (2 x 10(6) CD34+ cell and/or 10 x 10(4) CGU-GM per kg) whether undertaken after the 4th dose of G-CSF or the 5th.
Kanold
J
J
Unité Bioclinique de Prélèvement et de Traitement des Cellules Souches Hématopoïétiques, Péduatrie CHU Clermont-Ferrand, France.
Berger
M
M
Halle
P
P
Rapatel
C
C
Schoepfer
C
C
de Lumley
L
L
Vannier
J P
JP
Lutz
P
P
Plantaz
D
D
Stéphan
J L
JL
Malpuech
G
G
Deméocq
F
F
D000293:Adolescent; D002648:Child; D002675:Child, Preschool; D005260:Female; D016179:Granulocyte Colony-Stimulating Factor / Q000494:pharmacology*; D019650:Hematopoietic Stem Cell Mobilization; D006801:Humans; D007223:Infant; D007700:Kinetics; D007938:Leukemia / Q000097:blood*; D008297:Male; D009369:Neoplasms / Q000097:blood*; D011446:Prospective Studies
D016179:Granulocyte Colony-Stimulating Factor