title: Bone marrow metastasis in Ewing's sarcoma and peripheral primitive neuroectodermal tumor: An immunohistochemical study.
abstract: Bone marrow metastases from small round cell tumors can present diagnostic difficulties. In this study, we assessed the value of immunohistochemistry, using two monoclonal antibodies to CD99, for the diagnosis of metastatic disease in bone marrow trephine specimens from patients with Ewing's sarcoma or primitive neuroectodermal tumor (PNET). The proportions of specimens showing metastases were 10.3% with routine staining and 20.7% with immunohistochemistry. The specimens that were negative on conventional light microscopy and positive with immunohistochemistry all showed other abnormalities. The results do not support the routine use of immunohistochemistry in specimens that are normal by conventional light microscopy, but indicate that useful information may be gained in cases where marrow histology is obscured by fibrosis, necrosis, or distortion artefact. Neither of the two antibodies tested was superior for this purpose.
authors.affiliation: Department of Paediatric Pathology, St. Michael's Hospital, Southwell Street, Bristol BS2 8EG, UK.
authors.forename: E J
authors.initials: EJ
authors.lastname: Lazda
authors.forename: P J
authors.initials: PJ
authors.lastname: Berry
mesh_terms: D000071443:12E7 Antigen; D000911:Antibodies, Monoclonal; D015703:Antigens, CD / Q000032:analysis; D001853:Bone Marrow / Q000737:chemistry / Q000473:pathology; D019046:Bone Marrow Neoplasms / Q000737:chemistry / Q000473:pathology / Q000556:secondary*; D001859:Bone Neoplasms / Q000737:chemistry / Q000473:pathology*; D015815:Cell Adhesion Molecules / Q000032:analysis; D004273:DNA, Neoplasm / Q000032:analysis; D006801:Humans; D007124:Immunoenzyme Techniques; D018241:Neuroectodermal Tumors, Primitive, Peripheral / Q000737:chemistry / Q000473:pathology / Q000556:secondary*; D016133:Polymerase Chain Reaction; D012512:Sarcoma, Ewing / Q000737:chemistry / Q000473:pathology / Q000556:secondary*
chemical_list: D000071443:12E7 Antigen; D000911:Antibodies, Monoclonal; D015703:Antigens, CD; C061828:CD99 protein, human; D015815:Cell Adhesion Molecules; D004273:DNA, Neoplasm