Cytokine induced expression of porcine inhibitor of apoptosis protein (iap) family member is regulated by NF-kappa B.
The inhibitor of apoptosis (iap) proteins belong to a gene family that protect certain cell to undergo programmed cell death in response to a variety of stimuli. By differential screening we have identified a cDNA clone, designated piap, in porcine aortic endothelial cells (PAEC) that turned out by sequence comparison to be a porcine member of the iap family. The expression of piap is strongly up-regulated upon treatment of endothelial cells (EC) with inflammatory cytokines TNF-alpha, IL-1 beta, and LPS. In EC these stimuli lead to the activation of nuclear transcription factor kappa B (NF-kappa B) that plays a role in countering TNF-alpha induced apoptosis. We demonstrate that adenovirus mediated overexpression of I kappa B alpha, an inhibitor of NF-kappa B suppresses the expression of piap in response to TNF-alpha suggesting that piap is one of the NF-kappa B regulated genes that operates to prevent programmed cell death of EC in inflammation.
Stehlik
C
C
Department of Vascular Biology and Thrombosis Research, VIRCC/University of Vienna, Austria.
de Martin
R
R
Binder
B R
BR
Lipp
J
J
D000595:Amino Acid Sequence; D000818:Animals; D001011:Aorta / Q000737:chemistry; D017209:Apoptosis / Q000235:genetics*; D051017:Apoptosis Regulatory Proteins; D001483:Base Sequence; D002478:Cells, Cultured; D016384:Consensus Sequence; D016207:Cytokines / Q000494:pharmacology*; D004268:DNA-Binding Proteins / Q000494:pharmacology; D004730:Endothelium, Vascular / Q000737:chemistry; D015870:Gene Expression; D020672:I-kappa B Proteins; D007375:Interleukin-1 / Q000494:pharmacology; D008070:Lipopolysaccharides / Q000494:pharmacology; D008969:Molecular Sequence Data; D000072000:NF-KappaB Inhibitor alpha; D016328:NF-kappa B / Q000037:antagonists & inhibitors / Q000494:pharmacology*; D011506:Proteins / Q000235:genetics*; D013552:Swine; D014409:Tumor Necrosis Factor-alpha / Q000494:pharmacology
D051017:Apoptosis Regulatory Proteins; D016207:Cytokines; D004268:DNA-Binding Proteins; D020672:I-kappa B Proteins; D007375:Interleukin-1; D008070:Lipopolysaccharides; D016328:NF-kappa B; D011506:Proteins; D014409:Tumor Necrosis Factor-alpha; C111061:apoptosis protein inhibitor, porcine; D000072000:NF-KappaB Inhibitor alpha