title: Comparison between radiation-induced cell cycle delay in lymphocytes and radiotherapy response in head and neck cancer.
abstract: A study was made evaluating the use of radiation-induced cell cycle delay in lymphocytes to predict tumour response to radiotherapy. Peripheral blood lymphocytes were isolated from whole blood from 49 patients with head and neck cancer before treatment with radiotherapy and from 25 healthy donors. The clinical response to radiotherapy was assessed at 0-2 months after treatment. The level of radiation-induced cell cycle delay was measured using flow cytometry after mitogen stimulation of lymphocytes. The analysis of ten normal donors gave no significant difference in variability between the intra-assay and the intra-donor samples. However, the cell cycle data for lymphocytes from these healthy donors showed significant inter-individual differences in G2 phase accumulation. Patients showing no response to radiotherapy had a high level of S-phase cells compared with partial (P < 0.001) and complete responders (P = 0.016). An inverse relationship was found when analysing the fraction of cells in G2 (P = 0.009 and 0.034 respectively). In general, healthy donors had similar cell cycle kinetics compared with the non-responders. In conclusion, the result indicates that radiation-induced cell cycle delay in lymphocytes is inversely correlated with tumour response to radiotherapy in head and neck cancer patients. However, the value of the present test for predicting individual tumour response is limited, because of assay variability and overlap between groups.
authors.affiliation: Department of Oncology-Pathology, Karolinska Hospital and Institute, Stockholm, Sweden.
authors.forename: R
authors.initials: R
authors.lastname: Tell
authors.forename: T
authors.initials: T
authors.lastname: Heiden
authors.forename: F
authors.initials: F
authors.lastname: Granath
authors.forename: A L
authors.initials: AL
authors.lastname: Borg
authors.forename: S
authors.initials: S
authors.lastname: Skog
authors.forename: R
authors.initials: R
authors.lastname: Lewensohn
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D002453:Cell Cycle / Q000528:radiation effects*; D004307:Dose-Response Relationship, Radiation; D005260:Female; D016195:G2 Phase; D006258:Head and Neck Neoplasms / Q000097:blood / Q000532:radiotherapy*; D006801:Humans; D008214:Lymphocytes / Q000166:cytology / Q000528:radiation effects*; D008297:Male; D008875:Middle Aged; D015999:Multivariate Analysis; D016196:S Phase