[Application of micronucleus test for the demonstration of antimutagenic properties of natural substances].
Authors have used a reliable and reproductible original model of controlled production of free radicals for the cytokinesis-block micronucleus assay. This mutagenicity test is of easy realization and quick interpretation. An experimental model is proposed to test mutagenic activity of free radical generators and antioxydant properties of many substances, such as ascorbic acid. Its addition to the culture medium showed a significant decrease in the rate of micronucleated cells exposed to the free radical generator. The study of other antioxydant compounds (beta-caroten and 5-hydroxyquinolin) have confirmed results previously obtained with ascorbic acid. Thus, the free radical generator coupled to the cytokinesis-block micronucleus assay represents a reliable test to study and evaluate the antioxydant power of natural substances.
Villani
P
P
Laboratoire de biogénotoxicologie (EA 1784), Faculté de Médecine, Marseille.
Orsière
T
T
Bartfai
E
E
Pélissier
A L
AL
Duffaud
F
F
Bouvenot
G
G
Botta
A
A
D000704:Analysis of Variance; D016587:Antimutagenic Agents / Q000494:pharmacology*; D001205:Ascorbic Acid / Q000494:pharmacology*; D005609:Free Radicals / Q000378:metabolism; D006801:Humans; D006912:Hydroxyquinolines / Q000494:pharmacology*; D015162:Micronucleus Tests; D019207:beta Carotene / Q000494:pharmacology*
D016587:Antimutagenic Agents; D005609:Free Radicals; D006912:Hydroxyquinolines; D019207:beta Carotene; D001205:Ascorbic Acid
Application du test des micronoyaux à la mise en évidence des propriétés antimutagènes de substances naturelles.