title: Growth hormone stimulates the degradation of calcium phosphate biomaterial by human monocytes macrophages in vitro.
abstract: This study investigated the effects of human growth hormone (hGH) on the monocyte/macrophage lineage, the first cell population involved in degradation of calcium phosphate ceramic after in vivo implantation. Monocytes isolated from human blood were cultured on biphasic calcium pellets (200 mg) for 8 days in the presence of lipopolysaccharides (LPS, 0.5 microgram/mL), hGH (10 and 50 ng/mL), or an association of LPS with hGH (10 and 50 ng/mL). Unlike LPS, hGH significantly decreased (about 25%) the total number of lacunae formed by monocytes. However, hGH induced the formation of lacunae with a greater surface area (about a 90% increase) as compared to the control. Finally, intense upmodulation (about a 250% increase) of lacuna surface area was observed in the presence of both soluble factors, suggesting that hGH and LPS act synergistically. In view of the development of a drug delivery system for hGH bone release, this study shows that hGH not only stimulates bone cells implicated in the synthesis of the extracellular matrix but also those involved in the early degradation of calcium phosphate biomaterial.
authors.affiliation: Laboratoire de recherche sur les matériaux d'intérêt biologique, Faculté de Chirurgie Dentaire, Nantes, France.
authors.forename: J
authors.initials: J
authors.lastname: Guicheux
authors.forename: S
authors.initials: S
authors.lastname: Kimakhe
authors.forename: D
authors.initials: D
authors.lastname: Heymann
authors.forename: P
authors.initials: P
authors.lastname: Pilet
authors.forename: G
authors.initials: G
authors.lastname: Daculsi
mesh_terms: D001672:Biocompatible Materials / Q000378:metabolism*; D002130:Calcium Phosphates / Q000378:metabolism*; D002478:Cells, Cultured; D019382:Human Growth Hormone / Q000494:pharmacology*; D006801:Humans; D066298:In Vitro Techniques; D008070:Lipopolysaccharides; D008264:Macrophages / Q000378:metabolism*; D009000:Monocytes / Q000378:metabolism*
chemical_list: D001672:Biocompatible Materials; D002130:Calcium Phosphates; D008070:Lipopolysaccharides; C485828:alpha-tricalcium phosphate; C485830:tetracalcium phosphate; D019382:Human Growth Hormone; C485838:calcium phosphate, monobasic, anhydrous; C020243:calcium phosphate; C485829:calcium phosphate, dibasic, anhydrous