[Flow cytometric analysis for the activation of peripheral immunoregulatory cells from patients with bronchial asthma].
By use of flow cytometry, we have investigated intracellular activated eosinophil cationic protein (ECP) in eosinophils and mitogen-induced cytokine production of T cells in peripheral blood from children with acute severe asthma. In addition, we measured ECP releasability (serum ECP/lysate ECP) as a maker of activated eosinophils. The monoclonal antibody EG2 (anti-activated ECP/EPX antibody) was used for measuring the amount of intracellular activated ECP. ECP releasability and mean fluorescence intensity (MFI) values of EG2-positive eosinophils increased at the time of asthmatic attack and reduced after treatment with improvement in peak expiratory flow. Furthermore, the frequency of T cells which produced IL-4, IL-5 and IFN-gamma stimulated with phorbol myristate acetate and ionomycin increased and reduced in parallel with MFI of EG2-positive cells. These observations suggest that flow cytometric analysis for intracellular ECP and mitogen-induced cytokine production reflects the activation of T cells in bronchial mucosa, and is useful for monitoring airway inflammation in bronchial asthma.
Aoyagi
M
M
Department of Pediatrics and Clinical Research. National Shimoshizu Hospital.
Shimojo
N
N
Sato
Y
Y
Watanabe
H
H
Sekine
K
K
Sannomiya
Y
Y
Nishimuta
T
T
Kohno
Y
Y
Niimi
H
H
D000293:Adolescent; D001249:Asthma / Q000276:immunology*; D015415:Biomarkers / Q000032:analysis; D001798:Blood Proteins / Q000032:analysis; D002648:Child; D002675:Child, Preschool; D016207:Cytokines / Q000096:biosynthesis*; D047091:Eosinophil Granule Proteins; D005260:Female; D005434:Flow Cytometry; D006801:Humans; D015847:Interleukin-4 / Q000096:biosynthesis; D015848:Interleukin-5 / Q000096:biosynthesis; D008213:Lymphocyte Activation; D008297:Male; D012260:Ribonucleases; D013601:T-Lymphocytes / Q000276:immunology*
D015415:Biomarkers; D001798:Blood Proteins; D016207:Cytokines; D047091:Eosinophil Granule Proteins; D015848:Interleukin-5; D015847:Interleukin-4; D012260:Ribonucleases