title: [Properties of extracellular products produced by group A streptococci isolated from patients with streptococcal toxic shock syndrome].
abstract: Extracellular products of group A streptococci isolated from patients with streptococcal toxic shock syndrome (STSS) were examined. The outline of the discussion of the 3 products are as follows; streptolysin O (SLO), proteinase and erythrogenic toxin. SLO and proteinase showed a relatively large amount of products more than erythrogenic toxin. SLO produced by group A streptococci isolated from the patient with STSS had an isoelectric point (pI) of 6.0 and a molecular weight of 64,000 and showed hemolytic activity in the presence of 2-mercaptoethanol (2-ME). Furthermore, the hemolytic activities of all components were inhibited by gamma-globulin and cholesterol. Proteinase had pIs of 8.7 and 8.9, and a molecular weight of 21,000. These data suggest that STSS clinical criteria probably reflects a characteristic of a large amount of products of individual S. pyogenes isolates.
authors.affiliation: Department of Bioorganic Chemistry, College of Environmental Health Azabu University.
authors.forename: J
authors.initials: J
authors.lastname: Suzuki
authors.forename: S
authors.initials: S
authors.lastname: Matsubuchi
authors.forename: E
authors.initials: E
authors.lastname: Yoshihara
authors.forename: S
authors.initials: S
authors.lastname: Kobayashi
authors.forename: K
authors.initials: K
authors.lastname: Okamura
authors.forename: T
authors.initials: T
authors.lastname: Okuda
authors.forename: M
authors.initials: M
authors.lastname: Okazaki
authors.forename: N
authors.initials: N
authors.lastname: Furuya
authors.forename: H
authors.initials: H
authors.lastname: Uchimura
authors.forename: T
authors.initials: T
authors.lastname: Tokunaga
authors.forename: S
authors.initials: S
authors.lastname: Shimazaki
mesh_terms: D001426:Bacterial Proteins / Q000032:analysis*; D010450:Endopeptidases / Q000032:analysis*; D005098:Exotoxins / Q000032:analysis*; D005110:Extracellular Space / Q000737:chemistry; D006801:Humans; D008565:Membrane Proteins; D012772:Shock, Septic / Q000382:microbiology*; D013290:Streptococcal Infections / Q000382:microbiology*; D013297:Streptococcus pyogenes / Q000378:metabolism*; D013301:Streptolysins / Q000032:analysis*
chemical_list: D001426:Bacterial Proteins; D005098:Exotoxins; D008565:Membrane Proteins; C117980:SpeA protein, Streptococcus pyogenes; D013301:Streptolysins; C027951:erythrogenic toxin; C025964:streptolysin O; D010450:Endopeptidases