[The reproductive characteristics of human adenovirus type 2 in cultures of lymphoblastoid cells with B and T phenotypes].
A comparative characteristic of the reproduction process of type 2 human adenovirus in several lines of lymphoblastoid cells of B- and T-phenotype is presented. Formation of hexone and infectious virus in the cells of Jurkat, MT4, Raji lines was rather intensive and approached to that in the culture of the permissive epithelium cells Hep-2. These indices were much lower in the cultures of cells B 95-8 and MT4/BIII LBK which were chronically infected by VEB and HIV, accordingly and produced them that can evidence for the interference of Ad and VEB or Ad and HIV under superinfection of cells. Cells of SEM line possessing T-phenotype, were apparently semi-permissive for Ad h2, though the low almost unchanged content of hexone and infectious virus remains in them for a rather long time: about 15 days. Thus, obtained data within analyzed series of experiments expand the present ideas about lymphotropicity of Ad as their important property realized at the level of cell and infected macroorganism.
Povnitsa
O Iu
OIu
Institute of Microbiology and Virology, National Academy of Sciences of Ukraine, Kyiv.
Diachenko
N S
NS
Chernomaz
A A
AA
Nosach
L N
LN
Rybalko
S L
SL
Gritsak
T F
TF
Beregovenko
V N
VN
Diadiun
S T
ST
D000260:Adenoviruses, Human / Q000235:genetics / Q000276:immunology / Q000502:physiology*; D000818:Animals; D000956:Antigens, Viral / Q000032:analysis / Q000096:biosynthesis; D001402:B-Lymphocytes / Q000276:immunology / Q000821:virology*; D002213:Capsid / Q000032:analysis / Q000096:biosynthesis; D036022:Capsid Proteins; D002460:Cell Line; D002478:Cells, Cultured; D015967:Gene Expression Regulation, Viral / Q000502:physiology; D006801:Humans; D013601:T-Lymphocytes / Q000276:immunology / Q000821:virology*; D013997:Time Factors; D014776:Virus Cultivation / Q000379:methods; D014779:Virus Replication / Q000502:physiology*
D000956:Antigens, Viral; D036022:Capsid Proteins; C018315:hexon capsid protein, Adenovirus
Osobennosti reproduktsii adenovirusa cheloveka tipa 2 v kul'turakh limfoblastoidnykh kletok B- i T-fenotipa.