title: Porcine somatotropin, dietary protein and energy effects on arginase and transaminase activities in pigs.
abstract: Two experiments were conducted with cross-bred barrows to determine the effect of somatotropin administration on liver enzyme activities. In the first experiment, pigs growing from 26 to 55 kg body weight were given two doses of pituitary porcine somatotropin (pST; 0 and 100 micrograms per kg body weight) and three levels of dietary energy (60, 80 and 100% of free choice intake). In the second experiment, pigs growing from 30 to 60 kg body weight were given two doses of recombinant porcine somatotropin (rpST; 0 and 100 micrograms per kg body weight) and five levels of dietary crude protein (110, 150, 190, 230 and 270 g crude protein/kg diet). Liver arginase (ARG, EC 3.5.3.1) and aspartate aminotransferase (AAT, EC 2.6.1.1) activities were then determined in organ samples taken at slaughter time. Dietary energy did not change liver ARG. Activities of both ARG and AAT increased as dietary crude protein increased. Both pST and rpST decreased ARG, AAT and serum utrea nitrogen. There was a lack of interaction between rpST therapy and dietary protein on either ARG or AAT activities, suggesting that set nutritional states are not required for expression of pST effects.
authors.affiliation: Growth Biology Laboratory, United States Department of Agriculture-Agricultural Research Service, Beltsville Agricultural Research Center, MD 20705, USA.
authors.forename: R W
authors.initials: RW
authors.lastname: Rosebrough
authors.forename: T J
authors.initials: TJ
authors.lastname: Caperna
authors.forename: R G
authors.initials: RG
authors.lastname: Campbell
authors.forename: N C
authors.initials: NC
authors.lastname: Steele
mesh_terms: D000824:Animal Nutritional Physiological Phenomena; D000818:Animals; D001119:Arginase / Q000187:drug effects / Q000378:metabolism*; D015331:Cohort Studies; D004044:Dietary Proteins / Q000008:administration & dosage*; D002149:Energy Intake / Q000502:physiology*; D013006:Growth Hormone / Q000008:administration & dosage* / Q000494:pharmacology; D008099:Liver / Q000187:drug effects / Q000201:enzymology*; D008297:Male; D011897:Random Allocation; D011994:Recombinant Proteins / Q000008:administration & dosage / Q000494:pharmacology; D013552:Swine / Q000378:metabolism*; D000637:Transaminases / Q000187:drug effects / Q000378:metabolism*
chemical_list: D004044:Dietary Proteins; D011994:Recombinant Proteins; D013006:Growth Hormone; D000637:Transaminases; D001119:Arginase