title: Identification of two distinct subsets of bovine gamma delta T cells with unique cell surface phenotype and tissue distribution.
abstract: We describe the characterization of two subsets of bovine gamma delta T cells having distinct cell surface phenotype and tissue distribution. One population expresses the previously described 215,000 MW WC1 antigen and is negative for the cell-surface differentiation antigens CD2, CD4, and CD8. The second population expresses CD2 and CD8 but not WC1 and appears to have a T-cell receptor (TCR) rearrangement distinct from that of the WC1+ population. The WC1- population is found in large numbers in spleen and intestine. In addition, this subset is not recognized by a number of monoclonal antibodies (mAbs) specific for TCR families that are well represented in the WC1+ population. The results indicate that the gamma delta T-cell population in cattle is considerably larger than previously described and that this population can be subdivided into two distinct subsets based on cell-surface phenotype and tissue distribution.
authors.affiliation: International Livestock Research Institute Nairobi, Kenya.
authors.forename: N D
authors.initials: ND
authors.lastname: Machugh
authors.forename: J K
authors.initials: JK
authors.lastname: Mburu
authors.forename: M J
authors.initials: MJ
authors.lastname: Carol
authors.forename: C R
authors.initials: CR
authors.lastname: Wyatt
authors.forename: J A
authors.initials: JA
authors.lastname: Orden
authors.forename: W C
authors.initials: WC
authors.lastname: Davis
mesh_terms: D000818:Animals; D000954:Antigens, Surface / Q000032:analysis; D002417:Cattle / Q000276:immunology*; D005434:Flow Cytometry; D007124:Immunoenzyme Techniques; D016130:Immunophenotyping; D008198:Lymph Nodes / Q000276:immunology; D016692:Receptors, Antigen, T-Cell, gamma-delta / Q000032:analysis* / Q000235:genetics; D013154:Spleen / Q000276:immunology; D016176:T-Lymphocyte Subsets / Q000276:immunology*; D013950:Thymus Gland / Q000276:immunology; D014162:Transfection
chemical_list: D000954:Antigens, Surface; D016692:Receptors, Antigen, T-Cell, gamma-delta