Down-regulation of type I protein kinase A by transfection of human breast cancer cells with an epidermal growth factor receptor antisense expression vector.
MDA-468 human breast cancer cells overexpress the EGFR and exhibit a functional TGFalpha-EGFR autocrine pathway. Loss of EGFR expression following stable transfection with an antisense EGFR cDNA containing plasmid down-regulates type I cAMP-dependent protein kinase (PKAI) expression with acquisition of cell growth resistance to the PKAI inhibitor 8-Cl-cAMP. These results suggest that PKAI expression and function are controlled by a TGFalpha-EGFR autocrine pathway in human breast cancer cells overexpressing the EGFR.
Ciardiello
F
F
Dipartimento di Endocrinologia e Oncologia Molecolare e Clinica, Facoltà di Medicina e Chirurgia, Università degli Studi di Napoli Federico II, Italy.
Dixit
M
M
di Isernia
G
G
Damiano
V
V
Bianco
R
R
Bianco
A R
AR
Arteaga
C L
CL
Tortora
G
G
D015124:8-Bromo Cyclic Adenosine Monophosphate / Q000031:analogs & derivatives / Q000494:pharmacology; D001943:Breast Neoplasms / Q000201:enzymology*; D017868:Cyclic AMP-Dependent Protein Kinases / Q000037:antagonists & inhibitors* / Q000235:genetics; D016373:DNA, Antisense / Q000494:pharmacology*; D015536:Down-Regulation; D066246:ErbB Receptors / Q000235:genetics / Q000502:physiology*; D005260:Female; D006801:Humans; D014162:Transfection; D016211:Transforming Growth Factor alpha / Q000502:physiology; D014407:Tumor Cells, Cultured
D016373:DNA, Antisense; D016211:Transforming Growth Factor alpha; D015124:8-Bromo Cyclic Adenosine Monophosphate; C044258:8-chloro-cyclic adenosine monophosphate; D066246:ErbB Receptors; D017868:Cyclic AMP-Dependent Protein Kinases