Molecular cloning and expression of an anti-idiotype antibody mimicking a protective oligosaccharide of the parasite Schistosoma mansoni.
Genes encoding the heavy and light chains of an anti-idiotype antibody (AB2) mimicking a protective oligosaccharide of Schistosoma mansoni were cloned and expressed as a single-chain Fv fragment. The expression in a functional state was tested using the AB1. A specific binding between sFv and AB1 was observed. Immunization with the recombinant AB2 indicates its capacity to elicit anti-S. mansoni antibodies.
Petitprez
K
K
INSERM U167 Centre d'Immunologie et de Biologie Parasitaire, Institut Pasteur, Lille, France. karine.petitprez@pasteur-lille.fr
Grzych
J M
JM
Pierrot
C
C
Godin
C
C
Capron
A
A
Khalife
J
J
D000595:Amino Acid Sequence; D000818:Animals; D000888:Antibodies, Anti-Idiotypic / Q000235:genetics* / Q000276:immunology*; D000909:Antibodies, Helminth / Q000276:immunology*; D000937:Antigen-Antibody Reactions; D000947:Antigens, Helminth / Q000276:immunology; D001483:Base Sequence; D003001:Cloning, Molecular; D004926:Escherichia coli / Q000235:genetics; D007128:Immunoglobulin Fragments / Q000235:genetics / Q000276:immunology; D007143:Immunoglobulin Heavy Chains / Q000235:genetics / Q000276:immunology; D007147:Immunoglobulin Light Chains / Q000235:genetics / Q000276:immunology; D008969:Molecular Sequence Data; D009844:Oligosaccharides / Q000276:immunology*; D011993:Recombinant Fusion Proteins / Q000276:immunology; D012550:Schistosoma mansoni / Q000276:immunology*
D000888:Antibodies, Anti-Idiotypic; D000909:Antibodies, Helminth; D000947:Antigens, Helminth; D007128:Immunoglobulin Fragments; D007143:Immunoglobulin Heavy Chains; D007147:Immunoglobulin Light Chains; D009844:Oligosaccharides; D011993:Recombinant Fusion Proteins; C055966:immunoglobulin Fv