Effect of monensin on the synthesis of beta-D-xyloside-initiated glycosaminoglycan and its linkage region oligosaccharides in human skin fibroblasts.
Human skin fibroblasts were cultured with a fluorogenic xyloside, 4-methylumbelliferyl-beta-D-xyloside (Xyl-MU) as an initiator, and the effects of monensin, which destroys the normal structure of the Golgi complex, on the synthesis of Xyl-MU-initiated glycosaminoglycan (GAG-MU) and its linkage region oligosaccharides were investigated. When the cells were incubated with Xyl-MU in the presence of monensin, the synthesis of GAG-MU was inhibited. In addition, the synthesis of Galbeta1-3Galbeta1-4Xylbeta1-MU as an intermediate of GAG-MU was inhibited, whereas the synthesis of Galbeta1-4Xylbeta1-MU, which is formed prior to Galbeta1-3Galbeta1-4Xylbeta1-MU, was not. These results indicate that inhibition of GAG-MU synthesis by monensin occurs at the point where the second galactose is joined to Galbeta1-4Xylbeta1-MU.
Takagaki
K
K
Department of Biochemistry, Hirosaki University School of Medicine, Aomori.
Tazawa
T
T
Munakata
H
H
Nakamura
T
T
Endo
M
M
D002478:Cells, Cultured; D005347:Fibroblasts / Q000187:drug effects / Q000378:metabolism; D006025:Glycosaminoglycans / Q000096:biosynthesis*; D006027:Glycosides / Q000378:metabolism*; D006801:Humans; D006923:Hymecromone / Q000031:analogs & derivatives / Q000378:metabolism; D008985:Monensin / Q000494:pharmacology*; D009844:Oligosaccharides / Q000378:metabolism*; D012867:Skin / Q000187:drug effects* / Q000378:metabolism*
D006025:Glycosaminoglycans; D006027:Glycosides; D009844:Oligosaccharides; C010807:xylosides; D006923:Hymecromone; C014111:methylumbelliferyl-beta-D-xyloside; D008985:Monensin