title: Photosystem I is an early target of photoinhibition in barley illuminated at chilling temperatures.
abstract: Light-induced damage to photosystem I (PSI) was studied during low-light illumination of barley (Hordeum vulgare L.) at chilling temperatures. A 4-h illumination period induced a significant inactivation of PSI electron transport activity. Flash-induced P700 absorption decay measurements revealed progressive damage to (a) the iron-sulfur clusters FA and FB, (b) the iron-sulfur clusters FA, FB, and FX, and (c) the phylloquinone A1 and the chlorophyll AO or P700 of the PSI electron acceptor chain. Light-induced PSI damage was also evidenced by partial degradation of the PSI-A and PSI-B proteins and was correlated with the appearance of smaller proteins. Aggravated photodamage was observed upon illumination of barley leaves infiltrated with KCN, which inhibits Cu,Zn-superoxide dismutase and ascorbate peroxidase. This indicates that the photodamage of PSI in barley observed during low-light illumination at chilling temperatures arises because the defense against active oxygen species by active oxygen-scavenging enzymes is insufficient at these specific conditions. The data obtained demonstrate that photoinhibition of PSI at chilling temperatures is an important phenomenon in a cold-tolerant plant species.
authors.affiliation: Department of Plant Biology, Royal Veterinary and Agricultural University, Copenhagen, Denmark. set@kvl.dk
authors.forename: S E
authors.initials: SE
authors.lastname: Tjus
authors.forename: B L
authors.initials: BL
authors.lastname: Møller
authors.forename: H V
authors.initials: HV
authors.lastname: Scheller
mesh_terms: D003080:Cold Temperature; D001467:Hordeum / Q000528:radiation effects*; D008027:Light; D045342:Light-Harvesting Protein Complexes; D045322:Photosynthetic Reaction Center Complex Proteins / Q000737:chemistry / Q000528:radiation effects*; D045331:Photosystem I Protein Complex; D011189:Potassium Chloride / Q000737:chemistry
chemical_list: D045342:Light-Harvesting Protein Complexes; D045322:Photosynthetic Reaction Center Complex Proteins; D045331:Photosystem I Protein Complex; D011189:Potassium Chloride