title: The effect of radiation on the expression of intercellular adhesion molecule-1 of human adenocarcinoma cells.
abstract: OBJECTIVE
  To investigate the changes in antigenic expression of intercellular adhesion molecule-1 (ICAM-1) caused by ionizing radiation of cultured human adenocarcinoma cells.
  
  
  METHODS
  Human colonic BM314 and gastric MKN45 adenocarcinoma cells were irradiated to investigate the expression of ICAM-1 on the cell membrane and in the supernatant. In addition, the ICAM-1 gene expression (mRNA) was analyzed using a ICAM-1 cDNA as a probe.
  
  
  RESULTS
  The expression of ICAM-1 on the membrane was found to increase by irradiation. This effect was also observed in the supernatant. In addition, the irradiated cell population showed slight, but clear increases in ICAM-1 mRNA expression.
  
  
  CONCLUSIONS
  These results show that the enhancement of expression of ICAM-1 by radiation takes place at the ICAM-1 gene expression (mRNA) level. The results suggest that the low dose radiation may be useful for accumulating LFA-1 positive cytotoxic T lymphocytes (CTL) at the local tumor tissue, by which tumor cells may be attacked.
authors.affiliation: Department of Radiology, Sapporo Medical University, School of Medicine, Japan.
authors.forename: M
authors.initials: M
authors.lastname: Hareyama
authors.forename: K
authors.initials: K
authors.lastname: Imai
authors.forename: A
authors.initials: A
authors.lastname: Oouchi
authors.forename: H
authors.initials: H
authors.lastname: Takahashi
authors.forename: Y
authors.initials: Y
authors.lastname: Hinoda
authors.forename: M
authors.initials: M
authors.lastname: Tsujisaki
authors.forename: M
authors.initials: M
authors.lastname: Adachi
authors.forename: T
authors.initials: T
authors.lastname: Shonai
authors.forename: K
authors.initials: K
authors.lastname: Sakata
authors.forename: K
authors.initials: K
authors.lastname: Morita
mesh_terms: D000230:Adenocarcinoma / Q000276:immunology; D000951:Antigens, Neoplasm / Q000187:drug effects / Q000235:genetics / Q000378:metabolism / Q000528:radiation effects*; D000970:Antineoplastic Agents / Q000494:pharmacology; D005434:Flow Cytometry; D006801:Humans; D018799:Intercellular Adhesion Molecule-1 / Q000187:drug effects / Q000235:genetics / Q000378:metabolism / Q000528:radiation effects*; D007372:Interferons / Q000494:pharmacology; D012333:RNA, Messenger / Q000187:drug effects / Q000378:metabolism / Q000528:radiation effects; D011829:Radiation Dosage; D013274:Stomach Neoplasms / Q000276:immunology; D014407:Tumor Cells, Cultured / Q000187:drug effects / Q000528:radiation effects
chemical_list: D000951:Antigens, Neoplasm; D000970:Antineoplastic Agents; D012333:RNA, Messenger; D018799:Intercellular Adhesion Molecule-1; D007372:Interferons