title: BAC/YAC contigs from the H2-M region of mouse Chr 17 define gene order as Znf173-Tctex5-mog-D17Tu42-M3-M2.
abstract: A yeast artificial chromosome (YAC) contig from the C57BL/6 (H2(b)) mouse was created from the major histocompatibility complex (Mhc, H2 in mouse) class Ib subregion, H2-M. It spans approximately 1.2 megabase (Mb) pairs and unites the previous >1.5-Mb YAC contigs (Jones et al. 1995) into a single contig, which includes 21 Mhc class I genes distal to H2-T1. A bacterial artificial chromosome (BAC) contig from the 129 (H2(bc)) mouse, spanning approximately 600 kilobases, was also built from Znf173 (Afp, a gene for acid finger protein), through Tctex5 (t-complex testis expressed-5) and Mog (myelin oligodendrocyte glycoprotein), to H2-M2. Twenty-four sequence-tagged site (STS) markers were newly developed, and 35 markers were mapped in the YAC/BAC contigs, which define the marker order as Cen - Znf173 - Tctex5 - Mog - D17Tu42 - D17Mit232 - H2-M3 - D17Leh525 - H2-M2 - Tel. The gene order of Znf173 - Tctex5 - Mog - D17Tu42 is conserved between mouse and human, showing that the middle H2-M region corresponds to the subregion of the human Mhc surrounding HLA-A.
authors.affiliation: Howard Hughes Medical Institute, Departments of Microbiology and Biochemistry, University of Texas Southwestern Medical Center, 5323 Harry Hines Blvd., Dallas, TX 75235-9050, USA.
authors.forename: M
authors.initials: M
authors.lastname: Yoshino
authors.forename: H
authors.initials: H
authors.lastname: Xiao
authors.forename: E P
authors.initials: EP
authors.lastname: Jones
authors.forename: K
authors.initials: K
authors.lastname: Fischer Lindahl
mesh_terms: D000818:Animals; D002875:Chromosomes / Q000235:genetics*; D022202:Chromosomes, Artificial, Bacterial / Q000235:genetics*; D018244:Chromosomes, Artificial, Yeast / Q000235:genetics*; D020451:Contig Mapping; D017931:DNA Primers / Q000235:genetics; D004268:DNA-Binding Proteins / Q000235:genetics; D023061:Gene Order / Q000235:genetics*; D005805:Genes, MHC Class I / Q000235:genetics*; D005819:Genetic Markers; D015698:Genomic Library; D006183:H-2 Antigens / Q000235:genetics; D006680:HLA Antigens / Q000235:genetics; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D047908:Intracellular Signaling Peptides and Proteins; D051379:Mice; D008810:Mice, Inbred C57BL; D008869:Microtubule-Associated Proteins; D008969:Molecular Sequence Data; D009687:Nuclear Proteins / Q000235:genetics; D054645:Protein Phosphatase 1; D016324:Sequence Tagged Sites; D044767:Ubiquitin-Protein Ligases; D000509:alpha-Fetoproteins; D056427:t-Complex Genome Region
chemical_list: C097353:AFP protein, human; D017931:DNA Primers; D004268:DNA-Binding Proteins; D005819:Genetic Markers; D006183:H-2 Antigens; D006680:HLA Antigens; D047908:Intracellular Signaling Peptides and Proteins; D008869:Microtubule-Associated Proteins; D009687:Nuclear Proteins; D000509:alpha-Fetoproteins; C116196:PPP1R11 protein, human; C533391:Ppp1r11 protein, mouse; D044767:Ubiquitin-Protein Ligases; D054645:Protein Phosphatase 1