title: Bone and cartilage formation in an experimental model of distraction osteogenesis.
abstract: OBJECTIVE
  (a) To develop a reliable and reproducible system for distraction osteogenesis in the rat to establish a model for future investigations of bone repair and regeneration. (b) To describe and characterize the histological events in distraction osteogenesis in the rat and to determine whether cartilage development is a normal component of the process.
  
  
  METHODS
  Species-specific, longitudinal time study.
  
  
  METHODS
  Twenty rats underwent production of a middiaphyseal femoral osteotomy and application of a monolateral external fixator specifically designed for distraction. Animals were divided into five groups based on the time and extent of lengthening.
  
  
  RESULTS
  During distraction, gap tissue showed collagen bundles and fibroblasts that were oriented longitudinally to the direction of the distraction force. Woven bone appeared to be laid down on these collagen scaffolds, and the newly formed vascular sinuses appeared to be the sites from which bone formation was initiated within the distraction gap. All groups undergoing active distraction showed intramembranous ossification in the distraction gap and endochondral ossification peripherally. However, when distraction was discontinued, endochondral ossification was observed in the gap.
  
  
  CONCLUSIONS
  Distraction produces an environment in the distraction gap that suppresses the formation of cartilage. The formation of cartilage by injured periosteum, however, is obligatory and does not appear to be influenced by distraction. Bone formation within the distraction gap occurs where angiogenesis develops.
authors.affiliation: Hospital for Joint Diseases, New York, New York, USA.
authors.forename: L M
authors.initials: LM
authors.lastname: Jazrawi
authors.forename: R J
authors.initials: RJ
authors.lastname: Majeska
authors.forename: M L
authors.initials: ML
authors.lastname: Klein
authors.forename: E
authors.initials: E
authors.lastname: Kagel
authors.forename: L
authors.initials: L
authors.lastname: Stromberg
authors.forename: T A
authors.initials: TA
authors.lastname: Einhorn
mesh_terms: D000818:Animals; D001861:Bone Regeneration; D002356:Cartilage / Q000502:physiology*; D004195:Disease Models, Animal; D005269:Femur / Q000601:surgery; D008297:Male; D010012:Osteogenesis; D019857:Osteogenesis, Distraction; D010027:Osteotomy; D051381:Rats; D017207:Rats, Sprague-Dawley; D015203:Reproducibility of Results