Effectiveness of Menuzo's B2 medium with buffalo rat liver cells for development of in vitro matured/in vitro fertilized bovine oocytes.
OBJECTIVE
The effectiveness of two culture media on the development of bovine embryos in a buffalo rat liver (BRL) coculture system was investigated.


METHODS
Bovine oocytes were matured and fertilized in vitro, then cocultured, 25 per well, for 7 days in 500 microliters of modified M199 or modified Menuzo's B2 medium over a BRL cell monolayer at 39 degrees C in an atmosphere of 5% CO2 in air. Medium 199 was modified by the addition of 10% of (v/v) fetal bovine serum (FBS), 9 mg/ml bovine serum albumin, 2 mM glycine, 1 mM alanine, and 0.1 mM nonessential amino acids (NEAA). Menuzo's B2 medium was modified by the addition of 10% (v/v) FBS, 1 mM alanine, and 0.1 mM NEAA.


RESULTS
Modified Menuzo's B2 medium improved embryo development to the morula or blastocyst stage compared to modified M199 (121/353, 34.3%, versus 99/362, 27.3%, P < 0.05).


CONCLUSIONS
These results suggest that Menuzo's B2 medium with modifications in a BRL coculture system can provide a significant benefit for culture of early bovine embryos over the traditional use of medium 199.
Krisher
R L
RL
Department of Dairy Science, Virginia Polytechnic Institute and State University, Blacksburg 24061-0315, USA.
Gibbons
J R
JR
Gwazdauskas
F C
FC
D000818:Animals; D002417:Cattle; D002470:Cell Survival; D002478:Cells, Cultured; D018920:Coculture Techniques; D003470:Culture Media / Q000737:chemistry / Q000494:pharmacology*; D005314:Embryonic and Fetal Development; D005260:Female; D005307:Fertilization in Vitro / Q000379:methods*; D008099:Liver / Q000502:physiology; D051381:Rats; D011915:Rats, Inbred BUF
D003470:Culture Media