Vinorelbine: cell cycle kinetics and differential sensitivity of human lymphocyte subpopulations.
Antiproliferative activity induced by vinorelbine (VRB) was studied in whole blood cultures (WBC) and in lymphocyte subpopulations evaluated by the MAC (morphology-antibody-chromosome) method. In WBC a significant delay in cell cycle kinetics in VRB-treated cultures compared with controls (P < 0.001) was observed. The highest dose (1.0 microg/ml) arrested all cells at the first metaphase. Both WBC and isolated lymphocyte cultures showed a significant increase in the mitotic index (MI) in VRB-treated cultures compared with controls (P < 0.001). Moreover, a significant increase in the MI was found in VRB-treated CD4 cells compared with VRB-treated CD8 (P < 0.01) and B-lymphocytes (P < 0.001).
González-Cid
M
M
Departamento de Genética, Instituto de Investigaciones Hematológicas Mariano R. Castex, Academia Nacional de Medicina, Buenos Aires, Argentina.
Larripa
I
I
Slavutsky
I
I
D000328:Adult; D000972:Antineoplastic Agents, Phytogenic / Q000494:pharmacology*; D002453:Cell Cycle; D005260:Female; D006801:Humans; D066298:In Vitro Techniques; D016131:Lymphocyte Subsets / Q000166:cytology / Q000187:drug effects*; D008297:Male; D008677:Metaphase; D008875:Middle Aged; D008940:Mitotic Index / Q000187:drug effects; D014747:Vinblastine / Q000031:analogs & derivatives* / Q000494:pharmacology; D000077235:Vinorelbine
D000972:Antineoplastic Agents, Phytogenic; D014747:Vinblastine; D000077235:Vinorelbine