title: Impaired expression of noncollagenous bone matrix protein mRNAs during fracture healing in ascorbic acid-deficient rats.
abstract: In scorbutic patients, fractures are slow to heal because of impaired collagen synthesis. To investigate the influence of impaired collagen synthesis on the differentiation and proliferation of osteogenic and chondrogenic cells, we examined the expression of genes encoding bone matrix proteins, including osteonectin (ON), osteopontin (OPN), osteocalcin (OC), and matrix Gla protein (MGP), as differentiation markers for osteogenic and chondrogenic cells during fracture healing in Osteogenic Disorder Shionogi (ODS) rats, which have a hereditary defect in the ability to synthesize ascorbic acid (Asc). In ODS rats without Asc supplementation, intramembranous ossification was completely inhibited. Although a few fibroblast-like cells expressing ON mRNA were observed, no OPN mRNA-expressing cells were detected. During endochondral ossification, a small amount of metachromatic staining cartilage appeared at the fracture site, but there was no provisional calcification zone in the cartilage. Chondrocytes expressed ON and MGP mRNAs, but not OPN mRNA. When Asc was given to these rats, callus formation was soon detected around the fracture site, while OPN mRNA was expressed by differentiated osteoblasts and hypertrophic chondrocytes. Our data indicate that impaired collagen synthesis due to Asc deficiency inhibited the increase of ON and MGP mRNA-expressing cells as well as the appearance of OPN mRNA-expressing cells. Since OPN is considered to play an important role in normal and pathological mineralization, lack of OPN mRNA expression accompanying impaired collagen synthesis may have a role in defective mineralization and delayed fracture healing in scurvy.
authors.affiliation: Department of Pathology, Osaka University Medical School, Suita, Japan.
authors.forename: M
authors.initials: M
authors.lastname: Sugimoto
authors.forename: S
authors.initials: S
authors.lastname: Hirota
authors.forename: M
authors.initials: M
authors.lastname: Sato
authors.forename: H
authors.initials: H
authors.lastname: Kawahata
authors.forename: I
authors.initials: I
authors.lastname: Tsukamoto
authors.forename: N
authors.initials: N
authors.lastname: Yasui
authors.forename: Y
authors.initials: Y
authors.lastname: Kitamura
authors.forename: T
authors.initials: T
authors.lastname: Ochi
authors.forename: S
authors.initials: S
authors.lastname: Nomura
mesh_terms: D000818:Animals; D001206:Ascorbic Acid Deficiency / Q000235:genetics / Q000378:metabolism*; D002135:Calcium-Binding Proteins / Q000096:biosynthesis; D002454:Cell Differentiation / Q000235:genetics; D019902:Chondrocytes / Q000378:metabolism; D003094:Collagen / Q000096:biosynthesis*; D016326:Extracellular Matrix Proteins; D017102:Fracture Healing / Q000187:drug effects* / Q000235:genetics; D015675:Osteocalcin / Q000096:biosynthesis; D010012:Osteogenesis / Q000235:genetics; D015676:Osteonectin / Q000096:biosynthesis; D053495:Osteopontin; D010750:Phosphoproteins / Q000096:biosynthesis; D012333:RNA, Messenger / Q000032:analysis*; D051381:Rats; D012795:Sialoglycoproteins / Q000096:biosynthesis; D013997:Time Factors; D000097568:Matrix Gla Protein
chemical_list: D002135:Calcium-Binding Proteins; D003094:Collagen; D016326:Extracellular Matrix Proteins; D015675:Osteocalcin; D015676:Osteonectin; D053495:Osteopontin; D010750:Phosphoproteins; D012333:RNA, Messenger; D012795:Sialoglycoproteins; D000097568:Matrix Gla Protein; C506897:Spp1 protein, rat