[A method of determining the specific volume of liposomes with Turnbull blue].
A method for measuring the specific volume of liposomes by using ferricyanide as a maker is proposed. Ferricyanide is determined by the formation of Turnbull's blue. Advantages of the use of this reaction are an eightfold increase in the sensitivity and the possibility to determine the marker compound in the spectral region (730 nm) free of any considerable interference of natural compounds. Results of the use of this method for measuring the specific volume of liposomes prepared by ultrasonic treatment of a lipid suspension are reported.
Lagutina
L S
LS
Bach Institute of Biochemistry, Russian Academy of Sciences, Moscow, Russia.
Shteĭn-Margolina
V A
VA
Bondarenko
D I
DI
Shol'tz
K F
KF
D004396:Coloring Agents; D005292:Ferricyanides; D005295:Ferrocyanides; D008081:Liposomes; D008722:Methods; D008854:Microscopy, Electron; D012680:Sensitivity and Specificity; D014465:Ultrasonics
D004396:Coloring Agents; D005292:Ferricyanides; D005295:Ferrocyanides; D008081:Liposomes; C007931:hexacyanoferrate III; C042827:potassium iron(III) hexacyanoferrate (II)
Metod opredeleniia udel'nogo ob''ema liposom s pomoshch'iu Turnbulevoĭsini.