A two-dimensional 1H detected 13C NMR investigation of pyruvate metabolism in Halobacterium salinarium.
Two-dimensional 1H detected 13C NMR spectroscopy has been used to study the intracellular metabolism of [3-(13)C]pyruvate in Halobacterium salinarium. The method, resulting in considerable improvement in spectral resolution and signal-to-noise ratio, is well suited for studying transient metabolic intermediates. Pyruvate utilization by the bacterium is a double exponential function with rate constants of 49.13 and 4.67x10(-3) per min. The relative 13C enrichment is the fastest for C-3 glutamate. Glutamate C-4 labeling decreases initially and increases later on during incubation, while glutamine C-3 is high to begin with and exhibits a declining trend. The glutamate labeling indicates a high initial flux through pyruvate carboxylase and extensive randomizing of the label in the tricarboxylic acid cycle.
Majumdar
A
A
Chemical Physics Group, Tata Institute of Fundamental Research, Bombay, India.
Sonawat
H M
HM
D002952:Citric Acid Cycle; D019617:Halobacterium salinarum / Q000378:metabolism*; D009682:Magnetic Resonance Spectroscopy; D011773:Pyruvates / Q000378:metabolism*
D011773:Pyruvates