Stable production of human gastric lipase by chromosomal integration in the fission yeast Schizosaccharomyces pombe.
Strains of the fission yeast Schizosaccharomyces pombe have been constructed containing single or multiple chromosomally integrated copies of an expression cassette for production of human gastric lipase. Integrant strains of S. pombe secrete active lipase and are stable for lipase production over a minimum of 50 generations in non-selective media. Lipase activity levels for integrant strains containing up to three tandem copies of the expression cassette are strongly correlated with copy number of the cassette in both complete and minimal media. Lipase activity is higher in complete medium than in minimal medium. Strains carrying three chromosomally integrated expression cassette copies can be grown without selection in complete medium and are capable of significantly higher lipase activities than strains containing the expression cassette on a multicopy plasmid.
Smerdon
G R
GR
Department of Biological Sciences, University of Exeter, UK.
Walton
E F
EF
Aves
S J
SJ
D006801:Humans; D008049:Lipase / Q000096:biosynthesis* / Q000235:genetics; D010957:Plasmids; D011994:Recombinant Proteins / Q000096:biosynthesis*; D012568:Schizosaccharomyces / Q000235:genetics*; D013270:Stomach / Q000201:enzymology*; D014170:Transformation, Genetic
D011994:Recombinant Proteins; D008049:Lipase