title: Host cells in the spiral canal of Taenia solium (Cestoda) cysticerci.
abstract: The presence of host inflammatory cells inside the spiral canal of all viable Taenia solium cysts obtained from naturally infected pigs is described. Cells can penetrate into the vicinity of suckers and rostellum, although most appear damaged, suggesting that conditions in the canal are deleterious for them. These observations extend the localization of host inflammatory infiltrate to this intricate microniche, which may offer new approaches for the treatment of cysticercosis, based on a scolex-targeted action. The presence of host cells in the canal of cysts also poses the problem of the resulting contamination with host materials in studies using cysts extracts. As an example, host DNA contamination is readily detectable in genomic DNA isolated from T. solium and Taenia taeniaeformis cysts, as demonstrated by polymerase chain reaction amplification and subsequent sequencing of a segment of the 18S ribosomal gene.
authors.affiliation: Department of Immunology, Instituto de Investigaciones Biomédicas, Universidad Nacional Autónoma de México, D.F., México.
authors.forename: P
authors.initials: P
authors.lastname: de la Torre
authors.forename: M T
authors.initials: MT
authors.lastname: Merchant
authors.forename: K
authors.initials: K
authors.lastname: Willms
authors.forename: J P
authors.initials: JP
authors.lastname: Laclette
mesh_terms: D000818:Animals; D001483:Base Sequence; D003551:Cysticercosis / Q000469:parasitology / Q000473:pathology / Q000662:veterinary*; D003552:Cysticercus / Q000166:cytology*; D018509:DNA, Helminth / Q000737:chemistry* / Q000302:isolation & purification; D006790:Host-Parasite Interactions; D007249:Inflammation / Q000473:pathology / Q000662:veterinary; D008969:Molecular Sequence Data; D018482:Muscle, Skeletal / Q000469:parasitology / Q000473:pathology; D016133:Polymerase Chain Reaction; D012689:Sequence Homology, Nucleic Acid; D013552:Swine; D013553:Swine Diseases / Q000469:parasitology* / Q000473:pathology
chemical_list: D018509:DNA, Helminth