title: Effect of sciatic nerve transection or TTX application on enzyme activity in rat spinal cord.
abstract: To clarify the differential effects on spinal circuitry caused by physical vs functional disconnection from the periphery, we compared changes produced by 3-, 7- or 14-day unilateral sciatic axotomy or tetrodotoxin (TTX) nerve blockade on the abundance or activity of NADPH diaphorase (NDP), cytochrome oxidase (CO) and acid phosphatase (AP) in the spinal cord. Following axotomy, AP and NDP were decreased in the dorsal horn and increased in large cells in the dorsolateral motor nuclei while CO was decreased in ventral horn neuropil. TTX induced a decrease of CO in the ventral horn and NDP in the dorsal horn. This suggests that physical vs functional disconnection causes modulation of distinct intracellular pathways in sensory afferents, dorsal horn neurons and motoneurons.
authors.affiliation: Department of Anatomy, Wright State University, Dayton, OH 45435, USA.
authors.forename: P A
authors.initials: PA
authors.lastname: Carr
authors.forename: V
authors.initials: V
authors.lastname: Haftel
authors.forename: F J
authors.initials: FJ
authors.lastname: Alvarez
authors.forename: T C
authors.initials: TC
authors.lastname: Cope
authors.forename: R E
authors.initials: RE
authors.lastname: Fyffe
mesh_terms: D000135:Acid Phosphatase / Q000378:metabolism; D000818:Animals; D019771:Axotomy; D003576:Electron Transport Complex IV / Q000378:metabolism; D008297:Male; D009046:Motor Neurons / Q000201:enzymology / Q000502:physiology; D009252:NADPH Dehydrogenase / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D012584:Sciatic Nerve / Q000502:physiology*; D013116:Spinal Cord / Q000187:drug effects / Q000201:enzymology* / Q000502:physiology*; D013779:Tetrodotoxin / Q000494:pharmacology*
chemical_list: D013779:Tetrodotoxin; D009252:NADPH Dehydrogenase; D003576:Electron Transport Complex IV; D000135:Acid Phosphatase