Localization of the mGlu4a metabotropic glutamate receptor in rat cerebellar cortex.
The subcellular localization of the mGlu4a metabotropic glutamate receptor was investigated in rat cerebellum. At the light microscopical level, strong mGlu4a immunoreactivity was found in the molecular layer. A post-embedding immunogold method for electron microscopy revealed gold particles at the presynaptic sites of synapses made by parallel fiber terminals with dendritic spines of Purkinje cells. These observations support electrophysiological evidence indicating an autoreceptor function of mGlu4 receptors at these synapses.
Mateos
J M
JM
Department of Neurosciences, Faculty of Medicine and Dentistry, Basque Country University, Bilbao, Spain.
Azkue
J
J
Sarría
R
R
Kuhn
R
R
Grandes
P
P
Knöpfel
T
T
D000595:Amino Acid Sequence; D000818:Animals; D000918:Antibody Specificity; D002525:Cerebellar Cortex / Q000378:metabolism* / Q000648:ultrastructure*; D003712:Dendrites / Q000378:metabolism / Q000648:ultrastructure; D008297:Male; D016253:Microscopy, Immunoelectron; D008969:Molecular Sequence Data; D010446:Peptide Fragments / Q000235:genetics / Q000276:immunology; D017729:Presynaptic Terminals / Q000378:metabolism / Q000648:ultrastructure; D011689:Purkinje Cells / Q000378:metabolism / Q000648:ultrastructure; D051381:Rats; D017207:Rats, Sprague-Dawley; D018094:Receptors, Metabotropic Glutamate / Q000235:genetics / Q000276:immunology / Q000378:metabolism*; D013569:Synapses / Q000378:metabolism / Q000648:ultrastructure
D010446:Peptide Fragments; D018094:Receptors, Metabotropic Glutamate; C108823:metabotropic glutamate receptor 4