Organization around the dnaA gene of Streptococcus pneumoniae.
The dnaA gene region of Streptococcus pneumoniae was cloned and sequenced. A tRNA gene, seven ORFs and three DnaA box clusters were identified. The order of the genes and intergene regions found was tRNA(Arg)-orf1-DnaA box cluster 3-htrA-spoOJ-DnaA box cluster 2-dnaA-DnaA box cluster 1-dnaN-orfX-orfY. Five ORFs are homologous to known bacterial genes. The tRNA(Arg) gene and orf1, also called orfL, have already been described in pneumococci and have been reported to be preceded by the competence regulation locus comCDE. In Escherichia coli, htrA encodes a serine protease. In Bacillus subtilis, spoOJ plays a role in sporulation and partition. dnaA encodes an initiator replication protein, very well conserved in several bacteria and dnaN encodes the beta subunit of DNA polymerase III in E. coli. The function of orfX is unknown. The N-terminal part of another reading frame, orfY, revealed high homology with a GTP-binding protein, DnaA box clusters were found upstream and downstream from dnaA. The presence of two such clusters suggests that the chromosomal origin of S. pneumoniae is located within this region. The position of dnaA, and therefore the putative origin of replication, were localized on the physical map of S. pneumoniae.
Gasc
Anne-Marie
AM
Laboratoire de Microbiologie et de Génétique Moléculaire du CNRS, Université Paul Sabatier, 118 route de Narbonne, 31062 Toulouse Cedex, France.
Giammarinaro
Philippe
P
Laboratoire de Microbiologie et de Génétique Moléculaire du CNRS, Université Paul Sabatier, 118 route de Narbonne, 31062 Toulouse Cedex, France.
Richter
Stefan
S
Max-Planck-Institut für Molekulare Genetik, Ihnestr. 73, D-14195 Berlin, Germany.
Sicard
Michel
M
Laboratoire de Microbiologie et de Génétique Moléculaire du CNRS, Université Paul Sabatier, 118 route de Narbonne, 31062 Toulouse Cedex, France.
D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000235:genetics* / Q000378:metabolism; D002874:Chromosome Mapping; D003001:Cloning, Molecular; D004258:DNA Polymerase III / Q000235:genetics; D017931:DNA Primers / Q000235:genetics; D004269:DNA, Bacterial / Q000032:analysis* / Q000235:genetics; D004268:DNA-Binding Proteins / Q000235:genetics* / Q000378:metabolism; D004720:Endonucleases; D004926:Escherichia coli / Q000235:genetics; D005656:Fungal Proteins / Q000235:genetics; D005798:Genes, Bacterial; D006360:Heat-Shock Proteins; D008969:Molecular Sequence Data; D005810:Multigene Family; D016254:Mutagenesis, Insertional; D016366:Open Reading Frames; D033903:Periplasmic Proteins; D012347:RNA, Transfer, Arg / Q000235:genetics; D018085:Regulon; D018741:Replication Origin; D029701:Saccharomyces cerevisiae Proteins; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D012697:Serine Endopeptidases / Q000235:genetics; D013296:Streptococcus pneumoniae / Q000235:genetics* / Q000378:metabolism
D001426:Bacterial Proteins; D017931:DNA Primers; D004269:DNA, Bacterial; D004268:DNA-Binding Proteins; C066312:DnaA protein, Bacteria; D005656:Fungal Proteins; D006360:Heat-Shock Proteins; D033903:Periplasmic Proteins; D012347:RNA, Transfer, Arg; C107849:SAE2 protein, S cerevisiae; D029701:Saccharomyces cerevisiae Proteins; C101139:beta subunit, DNA polymerase III; D004258:DNA Polymerase III; D004720:Endonucleases; C061682:DegP protease; D012697:Serine Endopeptidases