title: Hepatitis G virus: prevalence and sequence analysis in blood donors of São Paulo, Brazil.
abstract: OBJECTIVE
  Hepatitis G virus (HGV) is a recently discovered viral agent transmitted by blood, which was firstly identified in patients with acute or chronic liver disease. HGV prevalence in US blood donors was recently found to average 1-2%. We report a much higher HGV frequency among blood donors of São Paulo, Brazil.
  
  
  METHODS
  200 serum samples were submitted to RT-PCR using primers directed to the 5' untranslated region and nonstructural 5A (NS5A) region. PCR products were analyzed by gel electrophoresis and Southern blot hybridization.
  
  
  RESULTS
  Of the 200 specimens, 18 (9%; 95% CI 5.4-13.8%) were positive by both sets of primers. Sequence analysis of the NS5A PCR products revealed a homology of 96.3%. Of the 18 HGV-positive samples, only one was positive for anti-HBc and all were anti-HCV- and HCV-RNA-negative.
  
  
  CONCLUSIONS
  Such a high prevalence of HGV in a nonsymptomatic population suggests that this is a benign agent.
authors.affiliation: Fundação Pró-Sangue Hemocentro de São Paulo, Brazil.
authors.forename: L
authors.initials: L
authors.lastname: Bassit
authors.forename: B
authors.initials: B
authors.lastname: Kleter
authors.forename: G
authors.initials: G
authors.lastname: Ribeiro-dos-Santos
authors.forename: G
authors.initials: G
authors.lastname: Maertens
authors.forename: E
authors.initials: E
authors.lastname: Sabino
authors.forename: D
authors.initials: D
authors.lastname: Chamone
authors.forename: W
authors.initials: W
authors.lastname: Quint
authors.forename: A
authors.initials: A
authors.lastname: Sáez-Alquézar
mesh_terms: D001483:Base Sequence; D001782:Blood Donors; D001938:Brazil / Q000453:epidemiology; D004279:DNA, Viral / Q000097:blood; D005260:Female; D018067:Flaviviridae / Q000737:chemistry / Q000235:genetics* / Q000302:isolation & purification; D006525:Hepatitis, Viral, Human / Q000453:epidemiology* / Q000235:genetics*; D006801:Humans; D008297:Male; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D015995:Prevalence; D017422:Sequence Analysis, DNA; D017361:Viral Nonstructural Proteins / Q000032:analysis
chemical_list: D004279:DNA, Viral; D017361:Viral Nonstructural Proteins