title: The sodA gene of Haemophilus ducreyi encodes a hydrogen peroxide-inhibitable superoxide dismutase.
abstract: Haemophilus ducreyi is the etiologic agent of the sexually transmitted disease chancroid, an ulcerative condition implicated in increased HIV transmission. There is increasing evidence for the roles of oxidative stress proteins including superoxide dismutase enzymes in the survival and persistence of pathogenic organisms within the host. The sodA gene of Haemophilus ducreyi was isolated from a genomic plasmid library on the basis of its ability to rescue the hydrogen peroxide hypersensitivity of an Escherichia coli sodA sodB strain. The H. ducreyi SodA protein also complemented the aerobic growth defect of the E. coli sodA sodB strain in minimal medium. The deduced amino-acid sequence of the H. ducreyi sodA gene product is 74 and 70% identical to the Mn-SODs of Haemophilus influenzae and E. coli, respectively. However, unlike Mn-SODs, the H ducreyi SodA protein was inhibited by hydrogen peroxide in native gels stained for SOD activity.
authors.affiliation: Department of Microbiology and Immunology, School of Medicine, University of North Carolina at Chapel Hill 27599, USA.
authors.forename: L R
authors.initials: LR
authors.lastname: San Mateo
authors.forename: K L
authors.initials: KL
authors.lastname: Toffer
authors.forename: T H
authors.initials: TH
authors.lastname: Kawula
mesh_terms: D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000037:antagonists & inhibitors / Q000235:genetics*; D001483:Base Sequence; D004269:DNA, Bacterial; D004926:Escherichia coli / Q000201:enzymology / Q000235:genetics; D005798:Genes, Bacterial; D005816:Genetic Complementation Test; D015698:Genomic Library; D006191:Haemophilus ducreyi / Q000201:enzymology* / Q000235:genetics; D006861:Hydrogen Peroxide / Q000378:metabolism; D008969:Molecular Sequence Data; D018384:Oxidative Stress; D010957:Plasmids; D013482:Superoxide Dismutase / Q000037:antagonists & inhibitors / Q000235:genetics*
chemical_list: D001426:Bacterial Proteins; D004269:DNA, Bacterial; D006861:Hydrogen Peroxide; C095500:SodA protein, Bacteria; D013482:Superoxide Dismutase