In vitro recombination of feline herpesvirus type 1.
We investigated the possibility of in vitro recombination of three different strains of feline herpesvirus type 1 (FHV-1), a virulent strain (C7805), a vaccine strain (F2), and a candidate vaccine strain with a deletion in the thymidine kinase gene (C7301dlTK). The results showed that recombination within different genotypes of FHV-1 strains could occur at a relatively high frequency ranging from 10.1% to 21.5% in vitro. These results indicate the generation of multiple mutant viruses by in vitro recombination experiments. The possibility for generating a virulent virus or novel type of FHV-1 by recombination in vivo is discussed.
Fujita
K
K
Department of Veterinary Microbiology, Graduate School of Agricultural and Life Sciences, University of Tokyo, Japan.
Maeda
K
K
Yokoyama
N
N
Miyazawa
T
T
Kai
C
C
Mikami
T
T
D000818:Animals; D002415:Cats; D002478:Cells, Cultured / Q000821:virology; D004279:DNA, Viral / Q000032:analysis / Q000235:genetics; D006564:Herpesviridae / Q000235:genetics* / Q000254:growth & development; D006566:Herpesviridae Infections / Q000235:genetics / Q000821:virology; D009154:Mutation / Q000235:genetics; D011995:Recombination, Genetic / Q000235:genetics; D014779:Virus Replication / Q000235:genetics
D004279:DNA, Viral