High resolution single strand conformation polymorphism analysis using formamide and ethidium bromide staining.
Single strand conformation polymorphism (SSCP) analysis using ethidium bromide can be improved by adding formamide as the denaturant. This gives higher resolution than previous SSCP methods; it had 100% sensitivity in the discrimination of 14 PCR samples from two different genes, even for a long fragment close to the upper limit of 250 base pairs. This modified procedure is a rapid, simple, safe, and yet highly sensitive method for detecting structural differences in DNA fragments.
Xie
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University Department of Medicine, University of Hong Kong, Hong Kong.
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Ma
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D002394:Catechol O-Methyltransferase / Q000235:genetics; D004591:Electrophoresis, Polyacrylamide Gel; D004996:Ethidium; D005456:Fluorescent Dyes; D005559:Formamides; D006801:Humans; D018807:Polymorphism, Single-Stranded Conformational; D011489:Protein Denaturation; D013194:Staining and Labeling / Q000379:methods; D016159:Tumor Suppressor Protein p53 / Q000235:genetics
D005456:Fluorescent Dyes; D005559:Formamides; D016159:Tumor Suppressor Protein p53; C031066:formamide; D002394:Catechol O-Methyltransferase; D004996:Ethidium