Isolation of human mannan binding lectin, serum amyloid P component and related factors from Cohn fraction III.
Cohn Fraction III, a waste product of plasma fractionation, is a source of several proteins involved in host defence as part of the innate immune system. Here a procedure is described for purifying mannan binding lectin and serum amyloid P component to virtual homogeneity from Cohn Fraction I + III, and their separation from other mannan-binding factors and from C-reactive protein. It is suggested that products derived from Cohn Fraction III could have therapeutic potential, either for specific substitution therapy or as a combined package of innate immune factors.
Kilpatrick
D C
DC
Department of Transfusion Medicine, Edinburgh, UK.
D002097:C-Reactive Protein / Q000032:analysis; D002352:Carrier Proteins / Q000097:blood* / Q000302:isolation & purification; D011232:Chemical Precipitation; D037222:Collectins; D004591:Electrophoresis, Polyacrylamide Gel; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D007070:Immunoglobulin A / Q000097:blood; D007074:Immunoglobulin G / Q000097:blood; D007075:Immunoglobulin M / Q000097:blood; D000683:Serum Amyloid P-Component / Q000032:analysis* / Q000302:isolation & purification
D002352:Carrier Proteins; D037222:Collectins; D007070:Immunoglobulin A; D007074:Immunoglobulin G; D007075:Immunoglobulin M; D000683:Serum Amyloid P-Component; D002097:C-Reactive Protein