Heparin-binding activity of feline herpesvirus type 1 glycoproteins.
Feline herpesvirus type 1 (FHV-1) possesses a very narrow host range, but the mechanism of its infection has not yet been analyzed. Heparan sulfate on the cell surface serves as a receptor for several herpesviruses. In this study, we determined that infection of FHV-1 is inhibited by addition of soluble heparin in cells cultures. Using heparin-affinity column, it was shown that FHV-1 gC is a major heparin-binding protein, and FHV-1 gB weakly binds to heparin, but FHV-1 gD does not. Furthermore, the FHV-1 gC expressed in insect cells can also bind to heparin despite of being immature glycosylation. Our results suggested that FHV-1 gC can bind to heparin as observed in other herpesviruses and that glycosylation of the gC does not affect its heparin-binding activity. In addition, mice immunized with the gC expressed in insect cells produced complement-dependent virus-neutralizing antibody.
Maeda
K
K
Department of Veterinary Microbiology, Faculty of Agriculture, Yamaguchi University, Yamaguchi-city, Japan.
Yokoyama
N
N
Fujita
K
K
Maejima
M
M
Mikami
T
T
D018138:Alphaherpesvirinae / Q000187:drug effects / Q000378:metabolism*; D000818:Animals; D000998:Antiviral Agents / Q000378:metabolism* / Q000494:pharmacology; D002415:Cats; D002460:Cell Line; D002846:Chromatography, Affinity; D002847:Chromatography, Agarose; D006493:Heparin / Q000378:metabolism* / Q000494:pharmacology; D051379:Mice; D008807:Mice, Inbred BALB C; D011993:Recombinant Fusion Proteins / Q000276:immunology / Q000378:metabolism; D018411:Spodoptera; D014759:Viral Envelope Proteins / Q000276:immunology / Q000378:metabolism*
D000998:Antiviral Agents; D011993:Recombinant Fusion Proteins; D014759:Viral Envelope Proteins; C090200:glycoprotein C, Felid herpesvirus 1; D006493:Heparin