title: Development of an enzyme-linked immunosorbent assay using recombinant chicken anemia virus proteins expressed in a baculovirus vector system.
abstract: Recombinant baculoviruses were constructed to express the putative proteins VP1, VP2 or VP3 of the chicken anemia virus (CAV). The recombinant VP1, VP2 or VP3 were detected by SDS-PAGE, and their molecular weights were 50, 30/27 and 16 kDa, respectively. The VP2 and VP3 reacted with sera from CAV-infected chickens in Western blot analysis and when used as an enzyme-linked immunosorbent assay (ELISA) antigen, but VP1 did not. Antibodies to CAV were detected, by ELISA using crude insect cell lysates containing VP2 or VP3, from 2 to 20 weeks or 2 to 7 weeks after CAV infection, respectively. These findings indicate that recombinant VP2 and VP3 expressed in the baculovirus vector system can be used as antigens to detect anti-CAV antibodies in ELISA.
authors.affiliation: Nippon Institute for Biological Science, Tokyo, Japan.
authors.forename: N
authors.initials: N
authors.lastname: Iwata
authors.forename: M
authors.initials: M
authors.lastname: Fujino
authors.forename: K
authors.initials: K
authors.lastname: Tuchiya
authors.forename: A
authors.initials: A
authors.lastname: Iwata
authors.forename: Y
authors.initials: Y
authors.lastname: Otaki
authors.forename: S
authors.initials: S
authors.lastname: Ueda
mesh_terms: D000818:Animals; D000914:Antibodies, Viral / Q000096:biosynthesis*; D000917:Antibody Formation; D000956:Antigens, Viral / Q000032:analysis* / Q000096:biosynthesis / Q000276:immunology; D016367:Baculoviridae; D015153:Blotting, Western; D002460:Cell Line; D017727:Chicken anemia virus / Q000276:immunology / Q000302:isolation & purification*; D002645:Chickens; D018173:Circoviridae Infections / Q000175:diagnosis / Q000276:immunology / Q000662:veterinary*; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D007564:Japan; D016133:Polymerase Chain Reaction; D011201:Poultry Diseases; D011994:Recombinant Proteins / Q000032:analysis / Q000096:biosynthesis / Q000276:immunology; D018411:Spodoptera; D013601:T-Lymphocytes; D014162:Transfection; D014764:Viral Proteins / Q000032:analysis* / Q000096:biosynthesis / Q000276:immunology
chemical_list: D000914:Antibodies, Viral; D000956:Antigens, Viral; D011994:Recombinant Proteins; D014764:Viral Proteins