title: Receptor-mediated maternofetal transfer of immunoglobulins. Inhibition of transport of anti-HIV-1 immunoglobulin by generic immunoglobulins in the in vitro perfused placenta.
abstract: OBJECTIVE
  The passage of immunoglobulin G (IgG) across the placenta is thought to involve Fc' receptors on the syncytiotrophoblast. To confirm the receptor dependency of this process we have studied the changes in the tissue content and transfer kinetics of immunoglobulins and hyperimmune serum to HIV (HIVIG) during in vitro dual placental perfusion.
  
  
  METHODS
  Isolated lobules of term placentae from normal pregnancies were perfused in a model of maternal and fetal circulation. The perfused tissue was compared to fresh tissue samples from the same placenta for the content of IgG, IgG subclasses, IgM, cytokeratin, human placental lactogen and SP1 antigen by immunohistochemistry and by protein elution.
  
  
  RESULTS
  The immunoglobulin staining faded by an average of 40% during the 1st hour of perfusion. In contrast, staining for cytokeratin, human placental lactogen and SP1 remained unchanged. During a 4-hour recycling of endogenous immunoglobulins in the maternal circulation, IgG and HIVIG crossed to the fetal side in a steady rate. The transport of HIVIG could be inhibited by preperfusion with an intravenous gammaglobulin preparation (IVIG).
  
  
  CONCLUSIONS
  The transfer of IgG across the placenta occurs in a steady state rate consistent with a receptor-mediated mechanism. Furthermore, inhibition of HIVIG maternofetal transfer by IVIG further establishes the receptor-mediated transfer of immunoglobulins through the placenta.
authors.affiliation: Department of Microbiology and Immunology, Albert Einstein College of Medicine, Bronx, NY 10461, USA.
authors.forename: M
authors.initials: M
authors.lastname: Landor
authors.forename: A
authors.initials: A
authors.lastname: Rubinstein
authors.forename: A
authors.initials: A
authors.lastname: Kim
authors.forename: T
authors.initials: T
authors.lastname: Calvelli
authors.forename: Y
authors.initials: Y
authors.lastname: Mizrachi
mesh_terms: D005260:Female; D006801:Humans; D007112:Immunity, Maternally-Acquired / Q000276:immunology*; D007074:Immunoglobulin G / Q000276:immunology / Q000378:metabolism; D007075:Immunoglobulin M / Q000276:immunology / Q000378:metabolism; D007136:Immunoglobulins / Q000378:metabolism; D016756:Immunoglobulins, Intravenous / Q000276:immunology / Q000378:metabolism*; D007150:Immunohistochemistry; D066298:In Vitro Techniques; D007633:Keratins / Q000378:metabolism; D010920:Placenta / Q000276:immunology* / Q000378:metabolism*; D010928:Placental Lactogen / Q000378:metabolism; D011247:Pregnancy; D011961:Receptors, Fc / Q000276:immunology / Q000378:metabolism*
chemical_list: C084715:HIV hyperimmune globulin; D007074:Immunoglobulin G; D007075:Immunoglobulin M; D007136:Immunoglobulins; D016756:Immunoglobulins, Intravenous; D011961:Receptors, Fc; C090698:SP1 antigen; D007633:Keratins; D010928:Placental Lactogen