A hirudin-sensitive, growth-related proteinase from human fibroblasts.
We have identified a hirudin-sensitive proteinase from human fibroblasts. Inhibition of this enzyme results in partial inhibition of cell growth in culture. The enzyme has a molecular weight of about 38000, and can be isolated by affinity chromatography with a hirudin-agarose matrix. The enzyme is apparently not closely related to thrombin, but does show a high level of amino acid sequence homology with fragments of a protein isolated from the synovial fluid of rheumatoid arthritis patients, which acts as a mitogen for human T lymphocytes.
Bush
D
D
School of Biological Sciences, University of Auckland, New Zealand.
Fritz
H
H
Knight
C
C
Mount
J
J
Scott
K
K
D000595:Amino Acid Sequence; D010450:Endopeptidases / Q000187:drug effects / Q000302:isolation & purification / Q000378:metabolism*; D005347:Fibroblasts / Q000201:enzymology*; D006629:Hirudins / Q000494:pharmacology*; D006801:Humans; D008969:Molecular Sequence Data; D011480:Protease Inhibitors / Q000494:pharmacology*
D006629:Hirudins; D011480:Protease Inhibitors; D010450:Endopeptidases; C112045:hirudin-sensitive fibroblast proteinase