title: Cellular expression of the cytolytic factor in earthworms Eisenia foetida.
abstract: Coelomic fluid of earthworms contains a 42 kDa protein designated CCF-1 (coelomic cytolytic factor 1), which accounts for approximately 40% of cytolytic activity of the entire coelomic fluid. CCF-1 was documented to be present on cells of the mesenchymal lining of the coelomic cavity as well as on free coelomocytes. Both cellular and humoral levels of CCF-1 were significantly increased after parenteral injection of endotoxin. Moreover, CCF-1 seems to be involved in cell mediated cytotoxicity, because cytotoxic activity is blocked in the presence of anti-CCF-1 monoclonal antibody (mAb).
authors.affiliation: Department of Immunology, Institute of Microbiology, Academy of Sciences of the Czech Republic, Vídenská, Prague.
authors.forename: M
authors.initials: M
authors.lastname: Bilej
authors.forename: P
authors.initials: P
authors.lastname: Rossmann
authors.forename: M
authors.initials: M
authors.lastname: Sinkora
authors.forename: R
authors.initials: R
authors.lastname: Hanusová
authors.forename: A
authors.initials: A
authors.lastname: Beschin
authors.forename: G
authors.initials: G
authors.lastname: Raes
authors.forename: P
authors.initials: P
authors.lastname: De Baetselier
mesh_terms: D000818:Animals; D003601:Cytotoxicity Tests, Immunologic; D003603:Cytotoxins / Q000096:biosynthesis*; D037102:Lectins; D008070:Lipopolysaccharides / Q000494:pharmacology; D009835:Oligochaeta / Q000276:immunology*
chemical_list: D003603:Cytotoxins; D037102:Lectins; D008070:Lipopolysaccharides; C094795:coelomic cytolytic factor 1, Eisenia fetida