Effect of heavy metals on Aedes aegypti (Diptera: Culicidae) larvae.
Studies were conducted to determine the biological effects of heavy metals on the development of Aedes aegypti. Embryos immersed in 32 ppm Cu or 5 ppm Cd did not hatch. The arrest of hatching was in part reversible by removal of the heavy metals. The mortality rate of third-instar larvae exposed to heavy metals for 24 h was metal and dose dependent; the 50% lethal concentration (LC50) endpoints were 3.1, 16.5, and 33 ppm for Hg, Cd, and Cu, respectively. Interestingly, a proportion of Aedes aegypti third-instar larvae exposed to either Cu or Cd for 24 h failed to produce a dissectable peritrophic matrix. This failure to produce a dissectable peritrophic matrix also was metal and dose dependent. These results are discussed in the context of Aedes aegypti as a model system for investigating the molecular biological effects of heavy metals in aquatic insects.
Rayms-Keller
A
A
Department of Microbiology, Colorado State University, Fort Collins 80523, USA.
Olson
K E
KE
McGaw
M
M
Oray
C
C
Carlson
J O
JO
Beaty
B J
BJ
D000330:Aedes / Q000187:drug effects* / Q000254:growth & development; D000818:Animals; D002104:Cadmium / Q000633:toxicity; D003300:Copper / Q000633:toxicity; D004064:Digestive System / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D004435:Eating / Q000187:drug effects; D004847:Epithelial Cells / Q000187:drug effects / Q000378:metabolism / Q000473:pathology; D005109:Extracellular Matrix / Q000187:drug effects / Q000473:pathology; D007814:Larva / Q000187:drug effects* / Q000254:growth & development; D008628:Mercury / Q000633:toxicity; D019216:Metals, Heavy / Q000633:toxicity*; D008675:Metamorphosis, Biological / Q000187:drug effects*; D015996:Survival Rate
D019216:Metals, Heavy; D002104:Cadmium; D003300:Copper; D008628:Mercury