Simultaneous determination of anabolic and catabolic steroid hormones in meat by gas chromatography-mass spectrometry.
A rapid and economical method for the determination in meat of androgens, estrogens, progestogens and corticoids, including some precursors and metabolites, has been developed. The extracted steroids are separated in a polar, a neutral, and a phenolic fraction by C8-SPE followed by a liquid-liquid extraction of the phenolates. Each fraction is separately purified by normal-phase SPE. The different steroid fractions can be analysed either together to obtain a comprehensive hormone pattern in one step or separately to enhance detection selectivity and sensitivity. Using a universally applicable silylation of the hydroxyl and keto groups, detection limits of 0.02-0.1 microg/kg are reached by GC-MS (EI) in the selected ion monitoring mode.
Hartmann
S
S
University of Hamburg, Institute of Biochemistry and Food Chemistry, Germany.
Steinhart
H
H
D000305:Adrenal Cortex Hormones / Q000032:analysis*; D045930:Anabolic Agents / Q000032:analysis*; D000728:Androgens / Q000032:analysis*; D000818:Animals; D002417:Cattle; D004967:Estrogens / Q000032:analysis*; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods*; D005965:Glucuronates / Q000378:metabolism; D006868:Hydrolysis; D008460:Meat / Q000032:analysis*; D010636:Phenols / Q000302:isolation & purification; D011372:Progestins / Q000032:analysis*; D012680:Sensitivity and Specificity; D013431:Sulfates / Q000378:metabolism
D000305:Adrenal Cortex Hormones; D045930:Anabolic Agents; D000728:Androgens; D004967:Estrogens; D005965:Glucuronates; D010636:Phenols; D011372:Progestins; D013431:Sulfates