The nitrite/elastin reaction: implications for in vivo degenerative effects.
Nitrite ion is a by-product of nitrogen oxides (nitric oxide and nitrogen dioxide) from cigarette smoke and is used as a preservative for curing meats. Therefore, study of the reaction of nitrite with elastin in vitro was undertaken. By colorimetric assay, reactivity of nitrite with insoluble elastin at neutral pH, 37 degrees C, and physiologic concentration was confirmed. In histochemical studies on in situ human aortic elastin, nitrite-treated sections displayed marked structural disruptions. Determinations of fluorescence and absorbance on nitrite-treated soluble bovine elastin revealed marked alterations of fluorescence, and increased UV and visible absorbance. Amino acid analysis confirmed that it reacted with tyrosine. The findings indicate that non-enzymatic nitration by nitrite may have deleterious effects on elastin in vivo and may provide insights into the pathogenesis of chronic elastin degenerative processes, including aortic aneurysms, pulmonary emphysema, and premature skin wrinkling, all of which have been well known to have associations with cigarette smoking.
Paik
D C
DC
Department of Surgery, Columbia University, St. Luke's/Roosevelt Hospital Center, New York, USA. mdt1@columbia.edu
Ramey
W G
WG
Dillon
J
J
Tilson
M D
MD
D000596:Amino Acids / Q000032:analysis; D000818:Animals; D001011:Aorta / Q000737:chemistry / Q000378:metabolism; D001014:Aortic Aneurysm / Q000378:metabolism / Q000473:pathology; D002417:Cattle; D004305:Dose-Response Relationship, Drug; D004549:Elastin / Q000737:chemistry / Q000378:metabolism* / Q000502:physiology; D006651:Histocytochemistry; D006801:Humans; D009573:Nitrites / Q000737:chemistry / Q000378:metabolism* / Q000494:pharmacology; D013050:Spectrometry, Fluorescence; D013056:Spectrophotometry, Ultraviolet
D000596:Amino Acids; D009573:Nitrites; D004549:Elastin