Induced expression of Thy-1 molecules on dermal endothelial cells in skin allografts.
We obtained evidence in a foregoing study that inducible Thy-1 on vascular endothelial cells functions as a possible vascular permeability modulator in the rat. We now report on the regulation and function of endothelial Thy-1 in skin allograft rejection in the rat. While no obvious expression of Thy-1 antigen on the vasculature can be seen in normal organs, dermal endothelial cells do express Thy-1 during allogeneic skin graft rejection and Freund's complete adjuvant (FCA)-induced inflammation. The antigen was weakly induced on tubular epithelial, but not on endothelial cells during kidney allograft rejection, and not in FCA-induced inflammation of the kidney. In contrast, Thy-1 antigen was not induced in the rejected lung or in FCA-induced inflammation of the lung. This pattern of Thy-1 regulation was transcriptionally regulated. Administration of anti-Thy-1 antibodies generated increased vascular permeability in skin allografts, although this procedure did not modulate survival of the grafts.
Takeuchi
E
E
Department of Pathology, Hokkaido University School of Medicine, Sapporo, Japan.
Ishikura
H
H
Ishizu
A
A
Harada
H
H
Kato
H
H
Yasuda
K
K
Yoshiki
T
T
D000818:Animals; D002199:Capillary Permeability / Q000276:immunology; D004727:Endothelium / Q000166:cytology*; D006085:Graft Survival / Q000276:immunology; D007249:Inflammation / Q000235:genetics / Q000473:pathology; D008297:Male; D012333:RNA, Messenger / Q000032:analysis; D051381:Rats; D011919:Rats, Inbred Strains; D016038:Skin Transplantation / Q000276:immunology / Q000473:pathology*; D018800:Thy-1 Antigens / Q000096:biosynthesis* / Q000235:genetics / Q000276:immunology
D012333:RNA, Messenger; D018800:Thy-1 Antigens