title: Pregnancy after human donor oocyte cryopreservation and thawing in association with intracytoplasmic sperm injection in a patient with ovarian failure.
abstract: OBJECTIVE
  To describe a pregnancy after human donor oocyte cryopreservation in association with intracytoplasmic sperm injection in a patient with ovarian failure.
  
  
  METHODS
  Department of Reproductive Medicine, CER Medical Institute, Buenos Aires, Argentina.
  
  
  METHODS
  A 48-year-old patient with ovarian failure.
  
  
  RESULTS
  Ten donated oocytes were cryopreserved. Survival after thawing was 30%. Three oocytes were microinjected, and two embryos were obtained. The fertilization rate was 66%, and embryo development was 100%. Both embryos were transferred to a patient who had received hormonal replacement therapy. The attempt was successful, and a pregnancy was achieved after the transfer.
  
  
  CONCLUSIONS
  In association with intracytoplasmic sperm injection, an adequate technique of freezing and thawing of human oocytes might achieve encouraging results in ART programs.
authors.affiliation: Department of Reproductive Medicine, CER Medical Institute, Buenos Aires, Argentina.
authors.forename: E
authors.initials: E
authors.lastname: Polak de Fried
authors.forename: J
authors.initials: J
authors.lastname: Notrica
authors.forename: M
authors.initials: M
authors.lastname: Rubinstein
authors.forename: A
authors.initials: A
authors.lastname: Marazzi
authors.forename: M
authors.initials: M
authors.lastname: Gómez Gonzalez
mesh_terms: D000328:Adult; D018997:Chorionic Gonadotropin, beta Subunit, Human / Q000097:blood; D015925:Cryopreservation; D004624:Embryo Transfer; D005260:Female; D005307:Fertilization in Vitro / Q000379:methods*; D006358:Hot Temperature; D006801:Humans; D007247:Infertility, Female / Q000628:therapy*; D008297:Male; D008845:Microinjections; D008875:Middle Aged; D018587:Oocyte Donation; D011247:Pregnancy; D011256:Pregnancy Outcome
chemical_list: D018997:Chorionic Gonadotropin, beta Subunit, Human