Towards the isolation of a human malignant extragonadal germ cell tumour-associated breakpoint in chromosome 11q13.
In a previous study we have defined a subgroup of human malignant extragonadal germ cell tumours that is characterized by complex translocations involving chromosomes 6 and 11 (Echten et al. 1995). Here we report (i) the use of fluorescent in situ hybridization, pulsed field gel electrophoresis and direct visual hybridization techniques to localize the tumour-associated breakpoint within band 11q13, and (ii) the construction of a phage library enriched for this region to facilitate genomic walks towards the breakpoint. Extensive breakpoint-flanking contigs were generated and within these contigs six candidate genes could be identified.
Sinke
R J
RJ
Department of Human Genetics, University Hospital Nijmegen, The Netherlands.
van Asseldonk
M
M
de Bruijn
D
D
Strijk
J A
JA
Merkx
G
G
Olde Weghuis
D
D
de Jong
B
B
Oosterhuis
J W
JW
Geurts van Kessel
A
A
D000328:Adult; D002874:Chromosome Mapping; D002880:Chromosomes, Human, Pair 11; D004273:DNA, Neoplasm; D015723:Gene Library; D015894:Genome, Human; D018237:Germinoma / Q000235:genetics*; D006801:Humans; D017403:In Situ Hybridization; D008297:Male; D013736:Testicular Neoplasms / Q000235:genetics*
D004273:DNA, Neoplasm