Gelatin nanoencapsulation of protein/peptide drugs using an emulsifier-free emulsion method.
The nanoencapsulation of a model protein drug, bovine serum albumin (BSA), using gelatin as the matrix material is reported. Nanoencapsulation was conducted using a modified water-in-oil (w/o) emulsion method, which is emulsifier-free and simple. The nanoencapsulation product, BSA-containing gelatin nanoparticles, is characterized in terms of nanoparticle morphology, size and size distribution, water content, and in vitro protein release. The BSA-containing gelatin nanoparticles obtained from this nanoencapsulation process are nearly spherical and have a log-normal size distribution. The average diameter of the BSA-containing gelatin nanoparticles is approximately 840 nm. They can absorb 51-72% of water. In vitro release experiments demonstrate that BSA has been successfully encapsulated in, and can be released from the gelatin nanoparticles. The release of BSA from the gelatin nanoparticulate matrix follows a diffusion-controlled release mechanism. It is found that temperature affects both the water content and the BSA release rate of the gelatin nanoparticles.
Li
J K
JK
Division of Pharmaceutics and Industrial Pharmacy, Arnold & Marie Schwartz College of Pharmacy and Health Sciences, Long Island University, Brooklyn, NY 11201, USA.
Wang
N
N
Wu
X S
XS
D002626:Chemistry, Pharmaceutical; D004058:Diffusion; D004339:Drug Compounding / Q000379:methods*; D004655:Emulsions / Q000378:metabolism*; D005780:Gelatin / Q000378:metabolism*; D010316:Particle Size; D012710:Serum Albumin, Bovine / Q000737:chemistry* / Q000493:pharmacokinetics; D013696:Temperature
D004655:Emulsions; D012710:Serum Albumin, Bovine; D005780:Gelatin