Occurrence of 13(S)-hydroxyoctadecadienoic acid in biological samples.
The oxygenated metabolite of linoleic acid, 13(S)-hydroxyoctadecadienoic acid has recently been shown to play a role in cellular regulation. To detect this molecule in biological systems, we recently developed a specific polyclonal antibody. Using this antibody, we report the presence of 13(S)-hydroxyoctadecadienoic acid in human urine, cell culture media, and untreated goat serum for the first time by a specific, sensitive, and rapid enzyme immunoassay. Furthermore, the enzyme linked immunosorbent assay data are verified by gas chromatography/mass spectrometry analysis of the same samples.
Spindler
S A
SA
Oxford Biomedical Research, Inc, Rochester Hills, MI 48309-3611, USA.
Clark
K S
KS
Blackburn
M L
ML
Bull
A W
AW
Reddy
R G
RG
D000818:Animals; D008401:Gas Chromatography-Mass Spectrometry; D006041:Goats; D006801:Humans; D007124:Immunoenzyme Techniques; D008041:Linoleic Acids / Q000032:analysis* / Q000097:blood / Q000652:urine; D012680:Sensitivity and Specificity; D013237:Stereoisomerism
D008041:Linoleic Acids; C024348:13-hydroxy-9,11-octadecadienoic acid