Simultaneous detection of two GFP spectral mutants during in vivo confocal microscopy of migrating Dictyostelium cells.
A method is described that allows simultaneous measurement of two spectrally distinguishable green fluorescent protein (GFP) mutants with a confocal microscope. In contrast to previously described methods, neither UV excitation nor repetition of scans is required. Therefore the method is well-suited to the long-time observation of living cells in three-dimensional microscopy and time series recording, as demonstrated with GFP-expressing Dictyostelium discoideum cells.
Zimmermann
T
T
Universität München, Germany.
Siegert
F
F
D000818:Animals; D001128:Argon; D002461:Cell Line, Transformed; D004023:Dictyostelium / Q000166:cytology* / Q000235:genetics* / Q000378:metabolism; D049452:Green Fluorescent Proteins; D007834:Lasers; D008164:Luminescent Proteins / Q000032:analysis* / Q000096:biosynthesis; D018613:Microscopy, Confocal; D009154:Mutation
D008164:Luminescent Proteins; D049452:Green Fluorescent Proteins; D001128:Argon