Transforming growth factor-beta1 (TGF-beta1) in dentine matrix. Ligand activation and receptor expression.
Transforming Growth Factor-beta (TGF-beta) and other members of this family of growth factors have been implicated in tooth development and dental tissue repair. This study aimed to investigate regulatory factors for TGF-beta1 in rabbit incisor dentine matrix and the expression of receptors for TGF-beta in dental tissues better to understand the control of its biological activity. Approximately half of the TGF-beta1 in dentine matrix was present in active form. TGF-beta1 was found in association with latency associated peptide (LAP), betaglycan and decorin in an isolated dentine matrix preparation. Immunohistochemistry showed strong staining for TGF-beta type I and II receptors in odontoblasts with more variable and weaker staining of other pulpal cells. Association of TGF-beta1 with betaglycan, decorin and LAP may regulate the availability and biological activity of this growth factor and influence its presentation to the TGF-beta type I and II receptors on odontoblasts. During dental tissue repair, such control processes will be important in regulating the biological effects of TGF-beta1 on cells of the dentine-pulp complex.
Smith
A J
AJ
Oral Biology, School of Dentistry, University of Birmingham, UK. a.j.smith.den@bham.ac.uk
Matthews
J B
JB
Hall
R C
RC
D000818:Animals; D058575:Decorin; D003804:Dentin / Q000166:cytology / Q000378:metabolism*; D005109:Extracellular Matrix / Q000378:metabolism; D016326:Extracellular Matrix Proteins; D007150:Immunohistochemistry; D008024:Ligands; D009804:Odontoblasts / Q000378:metabolism; D010446:Peptide Fragments; D011498:Protein Precursors; D011506:Proteins / Q000378:metabolism; D011509:Proteoglycans / Q000378:metabolism; D011817:Rabbits; D018125:Receptors, Transforming Growth Factor beta / Q000145:classification / Q000378:metabolism*; D015398:Signal Transduction; D016212:Transforming Growth Factor beta / Q000378:metabolism*; D053773:Transforming Growth Factor beta1
D058575:Decorin; D016326:Extracellular Matrix Proteins; D008024:Ligands; D010446:Peptide Fragments; D011498:Protein Precursors; D011506:Proteins; D011509:Proteoglycans; D018125:Receptors, Transforming Growth Factor beta; D016212:Transforming Growth Factor beta; D053773:Transforming Growth Factor beta1; C071736:betaglycan