Physical identification of a chromosomal locus encoding biosynthetic genes for the lipopeptide calcium-dependent antibiotic (CDA) of Streptomyces coelicolor A3(2).
Putative peptide-synthetase-encoding DNA fragments were isolated from the Streptomyces coelicolor A3(2) chromosome using a PCR-based approach and mapped to a single approximately 35 kb segment. In integrative transformation experiments, DNA fragments from this region disrupted production of the calcium-dependent antibiotic (CDA) and had sequences characteristic of non-ribosomal peptide synthetases, thus proving that the cda locus had been cloned.
Chong
Pei Pei
PP
Department of Biochemistry and Applied Molecular Biology, University of Manchester Institute of Science and Technology (UMIST), PO Box 88, Manchester M60 1QD, UK.
Podmore
Sylvia M
SM
Department of Biochemistry and Applied Molecular Biology, University of Manchester Institute of Science and Technology (UMIST), PO Box 88, Manchester M60 1QD, UK.
Kieser
Helen M
HM
John Innes Centre, Norwich NR4 7UH, UK.
Redenbach
Matthias
M
Department of Genetics, Genome Research Unit, Kaiserslautern University, 67663 Kaiserslautern, Germany.
Turgay
Kürsad
K
FB Chemie, Philipps-Universität, Hans-Meerwein-Strasse, 35032 Marburg, Germany.
Marahiel
Mohamed
M
FB Chemie, Philipps-Universität, Hans-Meerwein-Strasse, 35032 Marburg, Germany.
Hopwood
David A
DA
John Innes Centre, Norwich NR4 7UH, UK.
Smith
Colin P
CP
Department of Biochemistry and Applied Molecular Biology, University of Manchester Institute of Science and Technology (UMIST), PO Box 88, Manchester M60 1QD, UK.
D000595:Amino Acid Sequence; D001483:Base Sequence; D002874:Chromosome Mapping; D002876:Chromosomes, Bacterial; D017931:DNA Primers; D004269:DNA, Bacterial / Q000096:biosynthesis / Q000302:isolation & purification; D005798:Genes, Bacterial; D007476:Ionophores / Q000378:metabolism; D008970:Molecular Weight; D010453:Peptide Synthases / Q000235:genetics* / Q000378:metabolism; D010455:Peptides; D016133:Polymerase Chain Reaction; D015183:Restriction Mapping; D016415:Sequence Alignment; D013302:Streptomyces / Q000235:genetics* / Q000378:metabolism*; D014169:Transformation, Bacterial
C040487:CDA antibiotic; D017931:DNA Primers; D004269:DNA, Bacterial; D007476:Ionophores; D010455:Peptides; D010453:Peptide Synthases