IL-13 induces tyrosine phosphorylation of phospholipase C gamma-1 following IRS-2 association in human monocytes: relationship with the inhibitory effect of IL-13 on ROI production.
Here we analysed the involvement of tyrosine phosphorylation in the regulation of the initial molecular events induced by IL-13 to modulate TPA-triggered reactive oxygen intermediates (ROI) production. Our data indicate that treatment of monocytes with a protein tyrosine kinase inhibitor (herbimycin A) prevents IL-13-induced cAMP accumulation and subsequent ROI inhibition. We have previously demonstrated that cAMP accumulation depends on inositol phosphates hydrolysis (InsPs) and intracellular Ca2+ mobilisation. The inhibition of InsPs and intracellular Ca2+ release by herbimycin A suggests a primary role of tyrosine kinases upstream PLC activation. We further specify that IL-13 stimulates PLC-gamma 1 and IRS-2 tyrosine phosphorylation in human monocytes. We demonstrate for the first time that IL-13 induces the association of IRS-2 with PLC-gamma 1. We proposed here that PLC-gamma 1 is a new candidate recruited by IRS-2.
Sozzani
P
P
Laboratoire de l'Université P. Sabatier, CHU Rangueil, Toulouse, France. Patricia.Sozzani@rangueil.inserm.fr
Hasan
L
L
Séguélas
M H
MH
Caput
D
D
Ferrara
P
P
Pipy
B
B
Cambon
C
C
D016227:Benzoquinones; D002118:Calcium / Q000378:metabolism; D000242:Cyclic AMP / Q000378:metabolism; D006801:Humans; D015544:Inositol 1,4,5-Trisphosphate / Q000378:metabolism; D055504:Insulin Receptor Substrate Proteins; D018793:Interleukin-13 / Q000494:pharmacology*; D047908:Intracellular Signaling Peptides and Proteins; D007527:Isoenzymes / Q000378:metabolism*; D047029:Lactams, Macrocyclic; D009000:Monocytes / Q000187:drug effects*; D051966:Phospholipase C gamma; D010750:Phosphoproteins / Q000378:metabolism*; D010766:Phosphorylation; D011233:Precipitin Tests; D011485:Protein Binding; D011505:Protein-Tyrosine Kinases / Q000037:antagonists & inhibitors; D011809:Quinones / Q000494:pharmacology; D017382:Reactive Oxygen Species / Q000378:metabolism*; D016897:Respiratory Burst / Q000187:drug effects; D017828:Rifabutin / Q000031:analogs & derivatives; D010738:Type C Phospholipases / Q000378:metabolism*; D014443:Tyrosine / Q000378:metabolism
D016227:Benzoquinones; C526297:IRS2 protein, human; D055504:Insulin Receptor Substrate Proteins; D018793:Interleukin-13; D047908:Intracellular Signaling Peptides and Proteins; D007527:Isoenzymes; D047029:Lactams, Macrocyclic; D010750:Phosphoproteins; D011809:Quinones; D017382:Reactive Oxygen Species; D017828:Rifabutin; D014443:Tyrosine; C020754:herbimycin; D015544:Inositol 1,4,5-Trisphosphate; D000242:Cyclic AMP; D011505:Protein-Tyrosine Kinases; D010738:Type C Phospholipases; D051966:Phospholipase C gamma; D002118:Calcium