Monoclonal antibodies defining differentiation antigens of swine lymphoid and myeloid cells.
Monoclonal antibodies recognising swine leucocyte antigens were identified and the corresponding antigens were characterised by determining their tissue distribution and molecular weight as well as immunohistochemical staining. On the basis of these data, we suggest that two antibodies are specific for a molecules within a porcine orthologue of one of the human CDII/CD18 complexes. We suggest that two others recognise swine wCD21, that one may recognise swine wCD6 and that another recognises an antigen designated by the International Swine CD Workshop cluster SWC9. Other antibodies were obtained which are specific for swine macrophages or B cells, but CD or cluster assignments for these antigens have not been made. Detailed analysis of the antibodies is presented. It is proposed that these antibodies will be useful in studies of the pig immune system and of virus infection.
Whittall
J T
JT
Institute for Animal Health, Pirbright Laboratory, Pirbright, Surrey, UK. j.whittall@umds.ac.uk
Parkhouse
R M
RM
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D000943:Antigens, Differentiation / Q000032:analysis*; D001402:B-Lymphocytes / Q000502:physiology; D005434:Flow Cytometry; D007150:Immunohistochemistry; D051379:Mice; D008807:Mice, Inbred BALB C; D013552:Swine / Q000276:immunology*
D000911:Antibodies, Monoclonal; D000943:Antigens, Differentiation