title: Mapping of FASN and ACACA on two chicken microchromosomes disrupts the human 17q syntenic group well conserved in mammals.
abstract: Fatty acid synthase and Acetyl-CoA carboxylase are both key enzymes of lipogenesis and may play a crucial role in the weight variability of abdominal adipose tissue in the growing chicken. They are encoded by the FASN and ACACA genes, located on human Chromosome (Chr) 17q25 and on Chr 17q12 or 17q21 respectively, a large region of conserved synteny among mammals. We have localized the homologous chicken genes FASN and ACACA coding for these enzymes, by single-strand conformation polymorphism analysis on different linkage groups of the Compton and East Lansing consensus genetic maps and by FISH on two different chicken microchromosomes. Although synteny is not conserved between these two genes, our results revealed linkage in chicken between FASN and NDPK (nucleoside diphosphate kinase), a homolog to the human NME1 and NME2 genes (non-metastatic cell proteins 1 and 2), both located on human Chr 17q21.3, and also between FASN and H3F3B (H3 histone family 3B), located on human Chr 17q25. The analysis of mapping data from the literature for other chicken and mammalian genes indicates rearrangements have occurred in this region in the mammalian lineage since the mammalian and avian radiation.
authors.affiliation: Laboratoire de Génétique Cellulaire, INRA-CRT, Castanet-Tolosan, France.
authors.forename: F
authors.initials: F
authors.lastname: Pitel
authors.forename: V
authors.initials: V
authors.lastname: Fillon
authors.forename: C
authors.initials: C
authors.lastname: Heimel
authors.forename: N
authors.initials: N
authors.lastname: Le Fur
authors.forename: C
authors.initials: C
authors.lastname: el Khadir-Mounier
authors.forename: M
authors.initials: M
authors.lastname: Douaire
authors.forename: J
authors.initials: J
authors.lastname: Gellin
authors.forename: A
authors.initials: A
authors.lastname: Vignal
mesh_terms: D000103:Acetyl-CoA Carboxylase / Q000235:genetics*; D000818:Animals; D002642:Chick Embryo; D002874:Chromosome Mapping; D002886:Chromosomes, Human, Pair 17; D064429:Fatty Acid Synthases / Q000235:genetics*; D006801:Humans
chemical_list: D064429:Fatty Acid Synthases; D000103:Acetyl-CoA Carboxylase