The cellular distribution of GAP-43 immunoreactivity in human neocortical areas using immunofluorescence and confocal microscopy: post-ischemic influence.
The distribution of growth associated protein-43 (GAP-43) immunoreactive (IR) neurons were studied in human neocortical areas, using immunofluorescence and confocal microscopy. The GAP-43-IR cells were generally localised close to blood vessels, and contained fewer lipofuscin granules than GAP-43 negative cells. Quantification of the relative number of GAP-43-IR cells in control cases showed that the highest number of GAP-43-IR cells were present in layers III and V in the motor and visual cortices, fewer in the temporal cortex, and the lowest number in the frontal cortex. After general ischemia, GAP-43-IR cells were significantly reduced at various survival times, with the counts being lowest in the 1 week surviving case, and higher, but still subcontrol, in the 1 year post-ischemic case.
Akulinin
V A
VA
Dept. of Histology and Embryology, Omsk Medical Academy, Omsk, Russian Federation.
Belichenko
P V
PV
Dahlstrom
A
A
D000293:Adolescent; D000328:Adult; D002545:Brain Ischemia / Q000378:metabolism* / Q000473:pathology; D002540:Cerebral Cortex / Q000166:cytology / Q000378:metabolism*; D005260:Female; D019085:Fluorescent Antibody Technique, Direct; D019922:GAP-43 Protein / Q000378:metabolism*; D006801:Humans; D015151:Immunoblotting; D008297:Male; D018613:Microscopy, Confocal; D008875:Middle Aged; D009044:Motor Cortex / Q000166:cytology / Q000378:metabolism; D017966:Pyramidal Cells / Q000378:metabolism; D014793:Visual Cortex / Q000166:cytology / Q000378:metabolism
D019922:GAP-43 Protein