Rapid DNA quantification method in microplates using daunorubicine fluorescence quenching.
We have developed a rapid, sensitive and inexpensive method to quantify DNA in crude extracts or PCR reaction products using daunorubicine fluorescence quenching. We obtained a linear standard curve from 4.7 ng to 600 ng DNA and no interaction was observed in the presence of proteins.
Gagne
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M
Department of Biological Chemistry, Faculty of Medicine, Universite Laval, Ste Foy, Quebec G1K 7P4, Canada.
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D000818:Animals; D004247:DNA / Q000032:analysis / Q000378:metabolism; D004273:DNA, Neoplasm / Q000032:analysis* / Q000378:metabolism; D003630:Daunorubicin / Q000032:analysis* / Q000378:metabolism; D005456:Fluorescent Dyes / Q000032:analysis* / Q000378:metabolism; D008297:Male; D016133:Polymerase Chain Reaction; D012474:Salmon / Q000235:genetics; D012680:Sensitivity and Specificity; D013050:Spectrometry, Fluorescence / Q000379:methods; D013094:Spermatozoa / Q000737:chemistry; D014407:Tumor Cells, Cultured
D004273:DNA, Neoplasm; D005456:Fluorescent Dyes; D004247:DNA; D003630:Daunorubicin