Immunohistochemical localization of leptin receptor in the rat brain.
The distribution of leptin receptor in the rat brain was determined by immunocytochemistry and Western blotting. Strong leptin receptor immunoreactivity was detected in the arcuate, paraventricular and ventromedial nuclei of the hypothalamus, and lateral hypothalamic area. The olfactory bulb, neocortex, cerebellar cortex, dorsal raphe nucleus, inferior olive nucleus, nucleus of the solitary tract, dorsal motor nucleus of the vagus nerve also showed intense immunoreactivity. Western blotting analysis yielded a 120-kDa major band.
Shioda
S
S
Department of Anatomy, Showa University School of Medicine, Tokyo, Japan. shioda@med.showa-u.ac.jp
Funahashi
H
H
Nakajo
S
S
Yada
T
T
Maruta
O
O
Nakai
Y
Y
D000818:Animals; D001111:Arcuate Nucleus of Hypothalamus / Q000737:chemistry; D015153:Blotting, Western; D001923:Brain Chemistry / Q000502:physiology*; D002352:Carrier Proteins / Q000032:analysis*; D007026:Hypothalamic Area, Lateral / Q000737:chemistry; D007150:Immunohistochemistry; D008297:Male; D019579:Neocortex / Q000737:chemistry / Q000166:cytology; D009765:Obesity / Q000378:metabolism*; D011689:Purkinje Cells / Q000737:chemistry; D017966:Pyramidal Cells / Q000737:chemistry; D051381:Rats; D017207:Rats, Sprague-Dawley; D011956:Receptors, Cell Surface; D054411:Receptors, Leptin; D014630:Vagus Nerve / Q000737:chemistry / Q000166:cytology
D002352:Carrier Proteins; D011956:Receptors, Cell Surface; D054411:Receptors, Leptin