title: 22-Mb integrated physical and genetic map based on YAC/STS content spanning the interval DXS1125-DXS95 in human Xq12-q21.31.
abstract: A YAC/STS map has been assembled spanning 22 Mb across Xq12-q21.31, between markers DXS1125 and DXS95. In addition to the landmark loci for the X-inactivation center XIST and the ATRX, ATP7A, phosphoglycerate kinase, POU3F4, and choroideremia genes, the candidate disease gene regions for torsion dystonia 3 and two X-linked mental retardation syndromes are included. Also, the human voltage-dependent anion channel gene (HVDAC1) has been placed near DXS986. The current map incorporates 211 YACs from five different libraries, formatted with 185 STSs that comprise 26 genetic linkage markers, 60 newly-developed YAC-end STSs, and eight ESTs. The multiple clone coverage and average resolution of one STS per 120 kb provide resources for disease gene searches and are facilitating complete sequencing of the region.
authors.affiliation: Department of Molecular Microbiology, Washington University School of Medicine, St. Louis, MO 63110, USA. stevem@borcim.wustl.edu
authors.forename: S
authors.initials: S
authors.lastname: Mumm
authors.forename: C
authors.initials: C
authors.lastname: Jermak
authors.forename: P
authors.initials: P
authors.lastname: Waeltz
authors.forename: M
authors.initials: M
authors.lastname: Schmatz
authors.forename: J
authors.initials: J
authors.lastname: Terrell
authors.forename: B
authors.initials: B
authors.lastname: McCauley
authors.forename: M
authors.initials: M
authors.lastname: Shoemaker
authors.forename: A
authors.initials: A
authors.lastname: Srivastava
authors.forename: J
authors.initials: J
authors.lastname: Kere
authors.forename: R
authors.initials: R
authors.lastname: Nagaraja
mesh_terms: D001483:Base Sequence; D002503:Centromere; D002874:Chromosome Mapping / Q000379:methods*; D018244:Chromosomes, Artificial, Yeast; D017931:DNA Primers; D015723:Gene Library; D030342:Genetic Diseases, Inborn / Q000235:genetics*; D008040:Genetic Linkage; D005819:Genetic Markers; D006801:Humans; D016133:Polymerase Chain Reaction; D016615:Telomere; D014960:X Chromosome
chemical_list: D017931:DNA Primers; D005819:Genetic Markers