IgG anti-endothelial cell antibodies (AECA) in type I diabetes mellitus; induction of adhesion molecule expression in cultured endothelial cells.
AECA were detected in 25 of 71 patients with type 1 diabetes mellitus and in two of 33 healthy subjects. Patients with diabetes of < 1 year duration and those with long-standing disease had the highest levels of these antibodies. Inhibition studies suggest that at least part of the AECA reactivity is due to cross-reactive anti-ssDNA antibodies. AECA-positive sera were able to increase intercellular adhesion molecule-1 (ICAM-1) and E-selectin on human umbilical vein endothelial cells (HUVEC). Increased binding of polymorphonuclear (PMN) cells was also found to accompany raised E-selectin expression. Soluble ICAM-1 and E-selectin were also found to be increased in the sera of AECA-positive patients. An effect of AECA on endothelial cell function is suggested in diabetes mellitus.
Triolo
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G
Department of Internal Medicine, University of Palermo, Italy.
Triolo
G
G
Accardo-Palumbo
A
A
Carbone
M C
MC
Ferrante
A
A
Casiglia
D
D
Giardina
E
E
D000293:Adolescent; D001323:Autoantibodies / Q000097:blood*; D002448:Cell Adhesion / Q000502:physiology; D002478:Cells, Cultured; D002648:Child; D002675:Child, Preschool; D003922:Diabetes Mellitus, Type 1 / Q000097:blood* / Q000276:immunology; D019040:E-Selectin / Q000097:blood; D004730:Endothelium, Vascular / Q000378:metabolism* / Q000473:pathology; D006801:Humans; D007074:Immunoglobulin G / Q000097:blood*; D018799:Intercellular Adhesion Molecule-1 / Q000096:biosynthesis* / Q000097:blood; D009504:Neutrophils / Q000473:pathology / Q000502:physiology
D001323:Autoantibodies; D019040:E-Selectin; D007074:Immunoglobulin G; C087589:anti-endothelial cell antibody; D018799:Intercellular Adhesion Molecule-1