Measuring protein-protein interactions.
The binding of one protein to another provokes a variety of biophysical changes that can then be used as a measure of the binding reaction. Optical spectroscopy, particularly fluorescence, is the most flexible technique, but surface plasmon resonance biosensors, microcalorimetry and mass spectroscopy have recently shown significant development.
Lakey
J H
JH
Department of Biochemistry and Genetics, Medical School, University of Newcastle upon Tyne, UK. J.H.Lakey@ncl.ac.uk
Raggett
E M
EM
D001665:Binding Sites; D002151:Calorimetry; D002942:Circular Dichroism; D005453:Fluorescence; D013058:Mass Spectrometry; D011485:Protein Binding; D011506:Proteins / Q000737:chemistry / Q000378:metabolism*
D011506:Proteins