title: Structural characterization of the N-linked oligosaccharides derived from HIVgp120 expressed in lepidopteran cells.
abstract: The oligosaccharides of recombinant HIV gp120 expressed in lepidopteran Sf9 cells were analysed after hydrazine release by gel permeation and high pH anion exchange chromatography. N-Linked glycans were exclusively of the oligomannose series and no evidence for charged complex or hybrid type glycans was found. However a glycosylation reaction similar to those found in vertebrates was evident. The major glycoform of gp120, that comprised 30% of all the species analysed, was structurally identified by exoglycosidase digestion and found to be a core fucosylated structure, Manalpha1,6(Manalpha1,3)Manbeta1,4GlcNAc(Fucalpha1+ ++,6)GlcNAc. Further confirmation of the ability of lepidopteran cells to fucosylate N-linked glycans was provided by an in vitro analysis of this reaction using authentic oligosaccharide substrates.
authors.affiliation: Glycobiology Institute, Department of Biochemistry, University of Oxford, UK. terry@oxglua.glycob.ox.ac.uk
authors.forename: T D
authors.initials: TD
authors.lastname: Butters
authors.forename: B
authors.initials: B
authors.lastname: Yudkin
authors.forename: G S
authors.initials: GS
authors.lastname: Jacob
authors.forename: I M
authors.initials: IM
authors.lastname: Jones
mesh_terms: D000818:Animals; D002240:Carbohydrate Sequence; D002478:Cells, Cultured; D002845:Chromatography; D015699:HIV Envelope Protein gp120 / Q000737:chemistry*; D006834:Hydrazines; D008362:Mannosides / Q000737:chemistry; D008969:Molecular Sequence Data; D009844:Oligosaccharides / Q000737:chemistry*; D011994:Recombinant Proteins / Q000737:chemistry; D018411:Spodoptera
chemical_list: D015699:HIV Envelope Protein gp120; D006834:Hydrazines; D008362:Mannosides; D009844:Oligosaccharides; D011994:Recombinant Proteins; C029424:hydrazine