Reduced expression of interleukin-8 receptors A and B on polymorphonuclear neutrophils from persons with human immunodeficiency virus type 1 disease and pulmonary tuberculosis.
The expression of the two human interleukin (IL)-8 receptors, designated IL-8RA (CXCR-1) and IL-8RB (CXCR-2), on the surface of whole blood polymorphonuclear leukocytes (PMNL) was determined by use of receptor-specific monoclonal antibodies and flow cytometry. Sixteen subjects each were included in 4 study groups: healthy blood donors (ND), patients with pulmonary tuberculosis (TB), human immunodeficiency virus type 1-seropositive patients (HIV), and HIV-1-seropositive subjects with pulmonary tuberculosis (HIV/TB). A significant reduction in the percentage of PMNL expressing IL-8RA and IL-8RB and in their respective fluorescence intensities was found in TB, HIV, and HIV/TB groups compared with that obtained for the ND group. The greatest down-regulation of both receptors occurred in the HIV/TB group. Furthermore, associated with this reduced expression of IL-8 receptors was impairment of both intracellular calcium flux and migration of PMNL in response to IL-8 in a group of HIV/TB patients compared with that in healthy persons.
Meddows-Taylor
S
S
Medical Research Council AIDS Virus Research Unit, National Institute for Virology, Johannesburg, South Africa.
Martin
D J
DJ
Tiemessen
C T
CT
D017088:AIDS-Related Opportunistic Infections / Q000276:immunology* / Q000382:microbiology; D000328:Adult; D000911:Antibodies, Monoclonal / Q000276:immunology; D015703:Antigens, CD / Q000378:metabolism*; D000995:Antitubercular Agents / Q000627:therapeutic use; D018791:CD4 Lymphocyte Count; D002118:Calcium / Q000378:metabolism; D002634:Chemotaxis, Leukocyte; D005260:Female; D005434:Flow Cytometry; D015497:HIV-1; D006801:Humans; D016209:Interleukin-8 / Q000276:immunology / Q000494:pharmacology; D008214:Lymphocytes / Q000276:immunology / Q000378:metabolism; D008297:Male; D009504:Neutrophils / Q000166:cytology / Q000276:immunology* / Q000378:metabolism*; D019707:Receptors, Chemokine / Q000378:metabolism*; D018123:Receptors, Interleukin / Q000378:metabolism*; D023062:Receptors, Interleukin-8A; D023063:Receptors, Interleukin-8B; D014397:Tuberculosis, Pulmonary / Q000188:drug therapy / Q000276:immunology*
D000911:Antibodies, Monoclonal; D015703:Antigens, CD; D000995:Antitubercular Agents; D016209:Interleukin-8; D019707:Receptors, Chemokine; D018123:Receptors, Interleukin; D023062:Receptors, Interleukin-8A; D023063:Receptors, Interleukin-8B; D002118:Calcium