Redox potential measurements of plasma in patients undergoing coronary artery bypass graft and its clinical significance.
The apparent redox potentials (Em) of plasma as a marker of oxidant injury during coronary artery bypass graft (CABG) is determined, and their clinical significance is discussed. We measured plasma Em of normal volunteers (n = 20) and samples drawn at different time points from patients undergoing elective CABG (n = 60) directly and by adding 5 microl (20 mM) oxidants or reductants with known redox potential to plasma (95 microl), using a micro Pt/AgCl combination redox electrode. The Em value stays elevated up to 30 min during the surgery, after the administration of protamine it came down toward a more reduced state. Similar changes are seen with the lactate pyruvate ratio. Smaller changes of Em than normal are observed in plasma samples from patients treated with Aprotinin (antiprotease), Carmeda (heparin-coated) circuit and aspirin reflecting their protective effect. Redox potential (Em) measurements appear to be effective and useful in monitoring redox shifts wherever oxidative stress needs to be monitored.
Rao
S K
SK
Department of Surgery, Long Island Jewish Medical Center, L.I. Campus of the Albert Einstein College of Medicine, New Hyde Park, New York 11040, USA.
Palazzo
R S
RS
Metz
H N
HN
Wilson
D W
DW
Nikolic
S D
SD
Graver
M
M
Rao
P S
PS
D000925:Anticoagulants / Q000494:pharmacology; D007611:Aprotinin / Q000494:pharmacology; D001241:Aspirin / Q000494:pharmacology; D001026:Coronary Artery Bypass; D004594:Electrophysiology; D006493:Heparin / Q000494:pharmacology; D006494:Heparin Antagonists / Q000494:pharmacology; D006706:Homeostasis / Q000187:drug effects; D006801:Humans; D007431:Intraoperative Complications / Q000097:blood / Q000517:prevention & control*; D019344:Lactic Acid / Q000097:blood; D016877:Oxidants / Q000494:pharmacology; D010084:Oxidation-Reduction; D018384:Oxidative Stress; D010949:Plasma / Q000737:chemistry*; D011479:Protamines / Q000494:pharmacology; D019289:Pyruvic Acid / Q000097:blood; D015842:Serine Proteinase Inhibitors / Q000494:pharmacology
D000925:Anticoagulants; D006494:Heparin Antagonists; D016877:Oxidants; D011479:Protamines; D015842:Serine Proteinase Inhibitors; D019344:Lactic Acid; D019289:Pyruvic Acid; D006493:Heparin; D007611:Aprotinin; D001241:Aspirin