[The visualization of streptococcal cells by the use of fluorescein-bond dextran].
The method for the visualization of streptococcal cells, based on the phenomenon of binding between fluorescein isothiocyanate-labeled dextran and the surface structures of streptococci, is proposed. S.cricetus, S.sobrinus and S.faecalis strains were studied. The data obtained in this study were compared with the results of flow cytofluorimetry of these microbial cells. The stability of binding dextrans by microbes and the influence of ionic force on this process were determined at room temperature, +4 degrees C and -20 degrees C.
Kalinin
N L
NL
Gamaleya Research Institute of Epidemiology and Microbiology, Moscow, Russia.
Pronin
A V
AV
D001431:Bacteriological Techniques; D003470:Culture Media; D003911:Dextrans / Q000494:pharmacology; D005434:Flow Cytometry; D005452:Fluoresceins / Q000494:pharmacology; D005453:Fluorescence; D007202:Indicators and Reagents; D008970:Molecular Weight; D013291:Streptococcus / Q000166:cytology* / Q000187:drug effects
D003470:Culture Media; D003911:Dextrans; D005452:Fluoresceins; D007202:Indicators and Reagents; C052526:fluorescein-dextran
Vizualizatsiia kletok streptokokkov s pomoshch'iu dekstrana, sviazannogo s fliuorestseinom.