Does potassium ion concentration affect lung preservation?
To evaluate the influence of potassium ion concentration in an organ preservation solution on lung preservation, four types of solutions were produced. These preservation solutions consisted of sodium and potassium ions on the cation and phosphate ions on the anion with glucose. Contents of solutions were: (1) 155 mmol/l Na+, 0 mmol/l K+; (2) 150 mmol/l Na+, 5 mmol/l K+; (3) 20 mmol/l Na+, 135 mmol/l K+; and (4) 0 mmol/l Na+, 155 mmol/l K+. All four solutions possessed the same osmolarity, the same pH in the same temperature, and the same buffer action. Lungs were preserved at 7 degrees C in these solutions for 24 h and evaluated in a rat lung perfusion system with perfluorochemical emulsion. Pressure-limited perfusions with pressure-limited ventilation were carried out for 20 min before and after preservation. Thereafter, the recovery ratios of pulmonary arterial flow (Q), tidal volume (VT), oxygen tension of the pulmonary venous effluent (PPVO2), and wet-to-dry ratios were compared. The recovery ratios of Q were better in low, but not zero, potassium solutions, whereas wet-to-dry ratios were kept lower in high potassium solutions. No difference in pulmonary compliance or gas exchange was observed against a great change in potassium ion concentration. Therefore, the potassium ion concentration did not play a major role in lung preservation.
Ono
K
K
Department of Surgery I, Tokyo Women's Medical College, Japan.
Sone
Y
Y
Irie
T
T
Ohnuki
T
T
Nitta
S
S
D000818:Animals; D006863:Hydrogen-Ion Concentration; D008168:Lung; D008297:Male; D019188:Organ Preservation Solutions; D011188:Potassium; D011651:Pulmonary Artery / Q000502:physiology; D011659:Pulmonary Gas Exchange; D051381:Rats; D017208:Rats, Wistar; D012129:Respiratory Function Tests; D014655:Vascular Resistance
D019188:Organ Preservation Solutions; D011188:Potassium