Antisense oligonucleotides.
Antisense technology was developed to inhibit gene expression by utilizing an oligonucleotide complementary to the mRNA which encodes the target gene. There are a few possible mechanisms for the inhibitory effects of antisense oligonucleotides. Among them, degradation of mRNA by RNase H is considered to be the major mechanism of action for antisense oligonucleotides. This technique was originally used to elucidate the function of a target gene, but may also have therapeutic applications, provided it is designed carefully and properly.
Kashihara
N
N
Department of Medicine III, Okayama University Medical School, Japan. kashinao@med.okayama-u.ac.jp
Maeshima
Y
Y
Makino
H
H
D000818:Animals; D015316:Genetic Therapy; D006801:Humans; D007674:Kidney Diseases / Q000628:therapy; D009841:Oligonucleotides / Q000737:chemistry; D016376:Oligonucleotides, Antisense / Q000008:administration & dosage / Q000737:chemistry / Q000627:therapeutic use; D012333:RNA, Messenger / Q000378:metabolism; D016914:Ribonuclease H / Q000378:metabolism
D009841:Oligonucleotides; D016376:Oligonucleotides, Antisense; D012333:RNA, Messenger; D016914:Ribonuclease H