title: Nucleotide and predicted peptide sequence of feline interleukin-12 (IL-12).
abstract: Feline Interleukin-12 (IL-12) is a heterodimeric glycoprotein consisting of two disulfide linked subunits of about 40 kD (p40) and 35 kD (p35). It is a pleiotropic cytokine mediating biological activities on T- and NK-cells. One important function is the induction of a Th1 immune response. Here we report the cloning and sequencing of feline IL-12, the expression of the p40-protein in E. coli and production of monoclonal antibodies. At the nucleotide level, feline IL-12 shows between 87-90%, on the amino acid level between 82-87% identity to the bovine and human IL-12, respectively.
authors.affiliation: Dept. of Internal Veterinary Medicine, University of Zurich, Switzerland. danifehr@vetklinik.unizh.ch
authors.forename: D
authors.initials: D
authors.lastname: Fehr
authors.forename: G A
authors.initials: GA
authors.lastname: Dean
authors.forename: J
authors.initials: J
authors.lastname: Huder
authors.forename: Z
authors.initials: Z
authors.lastname: Fan
authors.forename: S
authors.initials: S
authors.lastname: Huettner
authors.forename: J W
authors.initials: JW
authors.lastname: Higgins
authors.forename: N C
authors.initials: NC
authors.lastname: Pedersen
authors.forename: H
authors.initials: H
authors.lastname: Lutz
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D000911:Antibodies, Monoclonal; D001483:Base Sequence; D002415:Cats; D002417:Cattle; D003001:Cloning, Molecular; D003593:Cytoplasm / Q000378:metabolism; D004926:Escherichia coli / Q000235:genetics; D006031:Glycosylation; D006801:Humans; D018664:Interleukin-12 / Q000235:genetics* / Q000276:immunology* / Q000378:metabolism; D051379:Mice; D008807:Mice, Inbred BALB C; D008969:Molecular Sequence Data; D011817:Rabbits; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism; D017421:Sequence Analysis; D017386:Sequence Homology, Amino Acid
chemical_list: D000911:Antibodies, Monoclonal; D011994:Recombinant Proteins; D018664:Interleukin-12