Serological reactivity of humans to a Vibrio cholerae common antigen.
Over the course of seven pandemics, Vibrio cholerae serotypes have varied. In 1992 the appearance of a new serotype, O139 Bengal, began the eighth cholera pandemic. Several new O139 antigens have been identified, yet a common V. cholerae antigen has not been described. In this study, a monoclonal antibody specific against an 18.7-kDa outer membrane antigen reacted in dotblot analysis with 292 epidemiologically diverse V. cholerae isolates including O1, non-O1, and O139 serotypes. Serum collected from volunteers experimentally challenged with V. cholerae O139, and rabbit antisera to V. cholerae O1, were reactive with the 18.7-kDa antigen by Western immunoblot. This is the first report that the 18.7-kDa antigen is present in V. cholerae O139.
Sciortino
C V
CV
Department of Veterans Affairs Medical Center, Louisville, KY 40206, USA.
D000911:Antibodies, Monoclonal / Q000276:immunology*; D000942:Antigens, Bacterial / Q000097:blood / Q000302:isolation & purification*; D015153:Blotting, Western; D002771:Cholera / Q000097:blood / Q000276:immunology*; D003720:Densitometry; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D012703:Serotyping; D014734:Vibrio cholerae / Q000276:immunology*
D000911:Antibodies, Monoclonal; D000942:Antigens, Bacterial