Determination of antibacterial quaternary ammonium compounds in lozenges by capillary electrophoresis.
A method for the specific determination of three quaternary ammonium compounds, benzalkonium chloride, cetylpyridinium chloride and dequalinium chloride, used as antibacterial agents in candy-based lozenges, is described based on capillary zone electrophoresis. It is shown that, following optimisation of buffer composition with respect to organic modifier concentration. pH and buffer concentration together with the inclusion of sodium dodecylsulphate as an ion-pairing agent in the case of dequalinium chloride, these analytes migrate in less than 5 min. The resultant electrophoretic peaks are sharp and readily quantified. The individual alkyl components of benzalkonium chloride can be resolved as can related impurities in dequalinium chloride lozenges. The quantitative characteristics of the assay method, based on peak areas normalised with respect to migration times, are reported and the method is compared with a previously published method based on liquid chromatography.
Taylor
R B
RB
School of Pharmacy, Robert Gordon University, Schoolhill, Aberdeen, UK.
Toasaksiri
S
S
Reid
R G
RG
D000890:Anti-Infective Agents / Q000032:analysis*; D001548:Benzalkonium Compounds / Q000032:analysis*; D002182:Candy; D002594:Cetylpyridinium / Q000032:analysis*; D003868:Dequalinium / Q000032:analysis*; D019075:Electrophoresis, Capillary / Q000379:methods*; D012680:Sensitivity and Specificity; D013607:Tablets
D000890:Anti-Infective Agents; D001548:Benzalkonium Compounds; D013607:Tablets; D002594:Cetylpyridinium; D003868:Dequalinium