Vagal involvement in dietary regulation of nutrient transport.
In omnivores, gradual alterations in dietary nutrient composition are observed. To efficiently absorb dietary nutrients these animals alter intestinal nutrient transporter expression to match the pattern of nutrient intake. This often involves reprogramming the crypt cell to express greater numbers of the relevant transport system. The aim of this study was to determine whether vagal afferents are involved in this adaptive process. Guinea pigs were habituated to a low-carbohydrate diet and then switched to a high-carbohydrate diet. The resultant increase in glucose transporter expression was assessed by determining rates of glucose transport in jejunal brush-border membrane vesicles. Ablation of vagal afferents was accomplished by application of capsaicin to exposed cervical vagi and confirmed using Fast blue tracer studies. We found that animals in which vagal afferents were ablated with capsaicin were unable to alter rates of glucose transport in response to an increase in dietary carbohydrate. This suggests that vagal afferents are involved in this adaptive process. These findings support a role for the vagus nerve in regulating intestinal transport function, which may be important to consider in clinical disease that involves the vagus nerve.
Bates
S L
SL
Gastrointestinal Research Group, Faculty of Medicine, University of Calgary, Alberta, Canada.
Sharkey
K A
KA
Meddings
J B
JB
D000222:Adaptation, Physiological; D000818:Animals; D001692:Biological Transport; D002211:Capsaicin / Q000494:pharmacology; D004040:Dietary Carbohydrates / Q000378:metabolism*; D017615:Enteric Nervous System / Q000187:drug effects / Q000502:physiology; D005502:Food; D005947:Glucose / Q000378:metabolism; D006168:Guinea Pigs; D007413:Intestinal Mucosa / Q000378:metabolism; D007422:Intestines / Q000294:innervation*; D008297:Male; D008871:Microvilli / Q000378:metabolism; D009004:Monosaccharide Transport Proteins / Q000187:drug effects / Q000378:metabolism; D014630:Vagus Nerve / Q000502:physiology*
D004040:Dietary Carbohydrates; D009004:Monosaccharide Transport Proteins; D005947:Glucose; D002211:Capsaicin