Cloning of a functional promoter of the human sodium/iodide-symporter gene.
We have cloned and sequenced genomic DNA from a human library extending 1300 bp upstream the 5'-untranslated sequence of the cDNA coding for the sodium/iodide symporter. In transient transfection assays this sequence exhibited promoter activity, which could be confined to nucleotides -443 to -395 relative to the ATG start codon. This minimal promoter, including a putative GC- and TATA- box, was preferentially activated in the rat thyroid cell line FRTL-5, but was also active in non-thyroidal cells, such as COS-7 and Chinese-hamster ovary, albeit to a markedly lower extent.
Behr
M
M
Abteilung Innere Medizin I, University of Ulm, Universitätsklinikum Ulm, Robert-Koch-Strasse 8, 89081 Ulm, Federal Republic of Germany.
Schmitt
T L
TL
Espinoza
C R
CR
Loos
U
U
D000818:Animals; D001483:Base Sequence; D016466:CHO Cells; D019556:COS Cells; D002352:Carrier Proteins / Q000235:genetics*; D002460:Cell Line; D003001:Cloning, Molecular; D006224:Cricetinae; D006801:Humans; D008156:Luciferases / Q000235:genetics; D008565:Membrane Proteins / Q000235:genetics*; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D011401:Promoter Regions, Genetic; D051381:Rats; D011993:Recombinant Fusion Proteins; D017422:Sequence Analysis, DNA; D027981:Symporters; D016385:TATA Box; D013961:Thyroid Gland; D014162:Transfection; D001616:beta-Galactosidase / Q000235:genetics; D000099209:Sodium-Iodide Symporters
D002352:Carrier Proteins; D008156:Luciferases; D008565:Membrane Proteins; D011993:Recombinant Fusion Proteins; D027981:Symporters; D001616:beta-Galactosidase; D000099209:Sodium-Iodide Symporters