title: Identification of the promoter region and the transcriptional regulatory sequence of the evgAS operon of Escherichia coli.
abstract: The evgAS operon of Escherichia coli encodes the EvgA response regulator and the EvgS sensory kinase, which are members of one of the two-component signal transduction systems of Escherichia coli. In this study, we identified the evg promoter and the EvgA-responsive element. Primer extension analysis found two evg transcriptional initiation sites, designated P1 (+1) and P2 (-10), and placed them 114 bp and 124 bp upstream of evgA, respectively. A gel retardation assay demonstrated that EvgA specifically bound to an inverted repeat located between -102 and -128 counting from P1. We also did a beta-galactosidase induction experiment using a promoter-probing vector and found that the EvgA-binding sequence was important to stimulate the evg promoter. These results suggest that the expression of evgAS is positively regulated by its own product, EvgA.
authors.affiliation: Department of Agricultural Chemistry, Kinki University, Nara, Japan.
authors.forename: H
authors.initials: H
authors.lastname: Tanabe
authors.forename: K
authors.initials: K
authors.lastname: Yamasaki
authors.forename: A
authors.initials: A
authors.lastname: Katoh
authors.forename: S
authors.initials: S
authors.lastname: Yoshioka
authors.forename: R
authors.initials: R
authors.lastname: Utsumi
mesh_terms: D001426:Bacterial Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D001483:Base Sequence; D017931:DNA Primers / Q000737:chemistry; D004269:DNA, Bacterial / Q000737:chemistry / Q000378:metabolism; D004587:Electrophoresis, Agar Gel; D004926:Escherichia coli / Q000737:chemistry / Q000235:genetics*; D029968:Escherichia coli Proteins; D015964:Gene Expression Regulation, Bacterial; D008969:Molecular Sequence Data; D009876:Operon / Q000235:genetics*; D016133:Polymerase Chain Reaction; D011401:Promoter Regions, Genetic; D011993:Recombinant Fusion Proteins / Q000737:chemistry; D015183:Restriction Mapping; D015398:Signal Transduction / Q000235:genetics; D014157:Transcription Factors / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D014158:Transcription, Genetic; D001616:beta-Galactosidase / Q000032:analysis
chemical_list: D001426:Bacterial Proteins; C065601:BvgA protein, Bacteria; D017931:DNA Primers; D004269:DNA, Bacterial; D029968:Escherichia coli Proteins; C489314:EvgA protein, E coli; D011993:Recombinant Fusion Proteins; D014157:Transcription Factors; C080662:bvgS protein, Bordetella pertussis; D001616:beta-Galactosidase