title: Adaptive response of human melanoma cells to methylglyoxal injury.
abstract: The effects of methylglyoxal on the growth of a line of human melanoma cells are investigated. Methylglyoxal inhibits cell growth in a dose-dependent manner and causes an increase in glyceraldehyde 3-phosphate dehydrogenase, and glyoxalase 1 and glyoxalase 2 specific activities. The cellular response to increasing concentrations of methylglyoxal in the culture medium is also studied by measuring L-lactate production, reduced-oxidized glutathione levels and apoptotic cell death. Methylglyoxal seems to promote a change of cell population phenotypic repertoire toward a more monomorphic phenotype. In conclusion, methylglyoxal seems to induce an enzymatic cellular response that lowers methylglyoxal levels and selects the most resistant cells.
authors.affiliation: Department of Basic and Applied Biology, University of L'Aquila, Italy.
authors.forename: F
authors.initials: F
authors.lastname: Amicarelli
authors.forename: T
authors.initials: T
authors.lastname: Bucciarelli
authors.forename: A
authors.initials: A
authors.lastname: Poma
authors.forename: P
authors.initials: P
authors.lastname: Aimola
authors.forename: C
authors.initials: C
authors.lastname: Di Ilio
authors.forename: A M
authors.initials: AM
authors.lastname: Ragnelli
authors.forename: M
authors.initials: M
authors.lastname: Miranda
mesh_terms: D017209:Apoptosis; D002455:Cell Division / Q000187:drug effects*; D003470:Culture Media; D053938:DNA Fragmentation; D005978:Glutathione / Q000378:metabolism; D019803:Glutathione Disulfide / Q000378:metabolism; D005987:Glyceraldehyde-3-Phosphate Dehydrogenases / Q000378:metabolism; D006801:Humans; D007791:Lactoylglutathione Lyase / Q000378:metabolism; D008545:Melanoma / Q000201:enzymology / Q000378:metabolism / Q000473:pathology*; D011765:Pyruvaldehyde / Q000633:toxicity*; D013869:Thiolester Hydrolases / Q000378:metabolism; D014407:Tumor Cells, Cultured; D000099301:Hydroxyacylglutathione Hydrolase
chemical_list: D003470:Culture Media; D005978:Glutathione; D019803:Glutathione Disulfide; D005987:Glyceraldehyde-3-Phosphate Dehydrogenases; D007791:Lactoylglutathione Lyase; D011765:Pyruvaldehyde; D013869:Thiolester Hydrolases; D000099301:Hydroxyacylglutathione Hydrolase