Molecular profiling of non-genotoxic hepatocarcinogenesis using differential display reverse transcription-polymerase chain reaction (ddRT-PCR).
The technique of differential display reverse transcription-polymerase chain reaction (ddRT-PCR) has been used to produce unique profiles of up-regulated and down-regulated gene expression in the liver of male Wistar rats following short term exposure to the non-genotoxic hepatocarcinogens, phenobarbital and WY-14,643. Animals were treated for 3 days, whereupon their livers were extracted and snap frozen. mRNA was prepared from the livers and used for ddRT-PCR. Individual bands from the differential displays were extracted and cloned. False positives were eliminated by dotblot screening and true positives then sequenced and identified.
Rockett
J C
JC
Molecular Toxicology Group, School of Biological Sciences, University of Surrey, Guildford, UK.
Esdaile
D J
DJ
Gibson
G G
GG
D000818:Animals; D002273:Carcinogens / Q000633:toxicity*; D004273:DNA, Neoplasm / Q000737:chemistry / Q000235:genetics; D008114:Liver Neoplasms, Experimental / Q000139:chemically induced* / Q000235:genetics; D008297:Male; D008958:Models, Molecular; D009153:Mutagens / Q000633:toxicity; D010634:Phenobarbital / Q000633:toxicity; D016133:Polymerase Chain Reaction; D011743:Pyrimidines / Q000633:toxicity; D012333:RNA, Messenger / Q000096:biosynthesis; D051381:Rats; D017208:Rats, Wistar
D002273:Carcinogens; D004273:DNA, Neoplasm; D009153:Mutagens; D011743:Pyrimidines; D012333:RNA, Messenger; C006253:pirinixic acid; D010634:Phenobarbital