Immunobiology of lipopolysaccharide (LPS) and LPS-derived immunoconjugates vaccinate mice against Salmonella typhimurium.
The immunobiology of lipopolysaccharide (LPS) of Salmonella typhimurium LT2-71 was studied in its native, modified and conjugated states using mice as the experimental model. An alkali-treated detoxified fraction of LPS (D-LPS) was found to be not only non-toxic but also equally immunogenic, like LPS. In addition D-LPS alone or conjugated with enterotoxin or hemolysin was also non-pyrogenic and non-indurogenic. The immunoprophylactic activity of D-LPS conjugates to a 100 ID50 challenge dose of S. typhimurium was also higher than that of detoxified LPS or native LPS.
Tiwari
R P
RP
Department of Microbiology, Panjab University, Chandigarh, India.
Gupta
W
W
Rishi
P
P
D000818:Animals; D000907:Antibodies, Bacterial / Q000096:biosynthesis; D001428:Bacterial Vaccines / Q000276:immunology*; D004768:Enterotoxins / Q000276:immunology; D006460:Hemolysin Proteins / Q000276:immunology; D018796:Immunoconjugates / Q000737:chemistry / Q000276:immunology* / Q000633:toxicity; D008070:Lipopolysaccharides / Q000276:immunology* / Q000633:toxicity; D051379:Mice; D011817:Rabbits; D012481:Salmonella Infections, Animal / Q000276:immunology / Q000517:prevention & control*; D012486:Salmonella typhimurium / Q000276:immunology*; D014611:Vaccination; D018074:Vaccines, Conjugate / Q000276:immunology
D000907:Antibodies, Bacterial; D001428:Bacterial Vaccines; D004768:Enterotoxins; D006460:Hemolysin Proteins; D018796:Immunoconjugates; D008070:Lipopolysaccharides; D018074:Vaccines, Conjugate