title: [Consequences for labile blood products of leukocyte depletion by whole blood filtration using the Leucoflex LST1 in-line filter. Evaluation of the Leucoflex LST1 filter].
abstract: The aim of this study is to evaluate the effects of whole blood filtration after a storage time of 20-24 hours at laboratory temperature using the in line filter Leucoflex LST1. The study concerns 49 blood donations in which we studied leukocyte depletion, proteins (IgG, IgA, IgM, haptoglobin, C3, C4), coagulation factors (fibrinogen, factors XII, XI, IX, VIII, V, proteins S and C, plasminogen, tPA, D-Dimers, PDF) at day 1, the parameters of conservation (ATP, 2-3 DPG, extra cellular potassium, haemolysis, pH) of red blood cell concentrates (RCCs) and bacteriological sterility at day 1 and 42. Despite a correct leukocyte depletion (mean depletion of 3.96 log), a 10 fold higher mean level of residual leukocytes/unit than with buffy coat poor RCC filtration (0.514.10(6) vs 0.051.10(6)) is observed. Moreover a lot of concentrates are not in accordance with French regulations (7/42 with more than 1.10(6) leukocytes/unit). The variation of the rates of IgG, IgA, IgM, haptoglobin, C4 and protein C is not significant. For the others there is a slight decrease with a mean level remaining in a physiological range. No sign of activation is noted. The sterility assays remain negative and the RCC conservation is not altered. In conclusion, even if the quality of the leukocyte depletion is not satisfactory in our study and has to be stated more precisely by multicenter studies, the whole blood filtration does not alter the quality of the derived components and allows us obtain RCC in a bigger volume and containing more haemoglobin than with the classical procedure after removing the buffy-coat [10].
authors.affiliation: Service des Contrôles et des HLA, CTSA Jean Julliard, Clamart.
authors.forename: G
authors.initials: G
authors.lastname: Janus
authors.forename: T
authors.initials: T
authors.lastname: Samson
authors.forename: R
authors.initials: R
authors.lastname: Faudon
authors.forename: C
authors.initials: C
authors.lastname: Renaudeau
authors.forename: M
authors.initials: M
authors.lastname: Joussemet
authors.forename: G
authors.initials: G
authors.lastname: Fabre
mesh_terms: D001769:Blood / Q000382:microbiology; D001781:Blood Component Removal / Q000295:instrumentation*; D001793:Blood Preservation; D001798:Blood Proteins / Q000032:analysis; D002469:Cell Separation / Q000295:instrumentation*; D004906:Erythrocyte Count; D005069:Evaluation Studies as Topic; D005374:Filtration / Q000295:instrumentation*; D006801:Humans; D007962:Leukocytes
chemical_list: D001798:Blood Proteins
vernacular_title: Conséquences sur les PSL de la déleucocytation par filtration du sang total avec le filtre intégré Leucoflex LST1. Evaluation du filtre Leucoflex LST1.