Co-localization of polar replication fork barriers and rRNA transcription terminators in mouse rDNA.
We investigated the replication of the region where transcription terminates in mouse rDNA. It contains a replication fork barrier (RFB) that behaves in a polar manner, arresting only replication forks moving in the direction opposite to transcription. This RFB consists of several closely spaced fork arrest sites that co-localize with the transcription terminator elements, known as Sal boxes. Sal boxes are the target for mTTF-I (murine transcription termination factor I). These results suggest that both termination of rRNA transcription and replication fork arrest may share cis-acting as well as trans-acting factors.
López-estraño
C
C
Centro de Investigaciones Biológicas, Consejo Superior de Investigaciones Científicas, Velázquez 144, Madrid, 28006, Spain.
Schvartzman
J B
JB
Krimer
D B
DB
Hernández
P
P
D000818:Animals; D004261:DNA Replication / Q000235:genetics*; D004275:DNA, Ribosomal / Q000235:genetics*; D051379:Mice; D009687:Nuclear Proteins / Q000378:metabolism; D012335:RNA, Ribosomal / Q000235:genetics*; D013728:Terminator Regions, Genetic; D000074482:Thyroid Nuclear Factor 1; D014157:Transcription Factors / Q000378:metabolism; D014407:Tumor Cells, Cultured
D004275:DNA, Ribosomal; D009687:Nuclear Proteins; D012335:RNA, Ribosomal; D000074482:Thyroid Nuclear Factor 1; D014157:Transcription Factors