title: Site-specific endonuclease SscL1 I from strain Staphylococcus species L1.
abstract: A site-specific endonuclease SscL1 I preparation has been isolated and purified to near homogeneity from the strain Staphylococcus sp. L1 without admixtures of other nuclease activity. DNA cleavage proceeds according to the scheme: 5'-G down arrow ANTC-3' 3'-CTNA up arrow G-5', and thus the isolated enzyme is an isoschizomer of restriction endonuclease HinfI and belongs to the second class of restriction endonucleases. SscL1 I works over a broad range of temperature and pH. The enzyme is characterized by high stability during storage.
authors.affiliation: Institute of Protein Research, Russian Academy of Sciences, Pushchino, Moscow Region, 142292 Russia. protres@sovam.com
authors.forename: L Y
authors.initials: LY
authors.lastname: Vasiljeva
authors.forename: L A
authors.initials: LA
authors.lastname: Zheleznaya
authors.forename: N I
authors.initials: NI
authors.lastname: Matvienko
mesh_terms: D004279:DNA, Viral / Q000378:metabolism; D015252:Deoxyribonucleases, Type II Site-Specific / Q000302:isolation & purification* / Q000378:metabolism; D013210:Staphylococcus / Q000201:enzymology*; D013379:Substrate Specificity
chemical_list: D004279:DNA, Viral; D015252:Deoxyribonucleases, Type II Site-Specific; C032229:GANTC-specific type II deoxyribonucleases