title: Association properties of betaB2- and betaA3-crystallin: ability to form dimers.
abstract: The beta-crystallins are a major constituent of the mammalian lens, where they associate into dimers, tetramers and higher order aggregates. Appropriate association of lens crystallins is important for lens transparency. To examine the associative properties of betaB2-crystallin, we have expressed mouse betaB2-crystallin using a baculovirus system. Recombinant mouse betaB2-crystallin has an estimated monomer molecular weight of 24 kDa by SDS-PAGE, appropriate immunoreactivity and appropriate secondary structure as assessed by circular dichroism analysis. The recombinant betaB2-crystallin associates into a homodimer with a weight average molecular mass of 39 kDa. The betaB2-crystallin homodimer has an estimated Kd of 5 x 10(-6) M, slightly greater than that of betaA3-crystallin, 0.8 x 10(-6) M. When recombinant betaB2-crystallin is combined with recombinant betaA3-crystallin, a heterodimer is formed within 10 min of incubation at room temperature. When equilibrium is reached in 4-6 h, approximately half of each crystallin associates into heterodimers. Subunit exchange between betaB2-crystallin and betaA3-crystallin occurs readily in the absence of any denaturing agents. Thus, rbetaA3-rbetaB2 heterodimer formation can occur under conditions similar to those found in the eye lens.
authors.affiliation: Ophthalmological Genetics and Clinical Services Branch, National Eye Institute, National Institutes of Health, Bethesda, MD 20892, USA.
authors.forename: J F
authors.initials: JF
authors.lastname: Hejtmancik
authors.forename: P T
authors.initials: PT
authors.lastname: Wingfield
authors.forename: C
authors.initials: C
authors.lastname: Chambers
authors.forename: P
authors.initials: P
authors.lastname: Russell
authors.forename: H C
authors.initials: HC
authors.lastname: Chen
authors.forename: Y V
authors.initials: YV
authors.lastname: Sergeev
authors.forename: J N
authors.initials: JN
authors.lastname: Hope
mesh_terms: D000818:Animals; D016367:Baculoviridae / Q000235:genetics; D002942:Circular Dichroism; D003459:Crystallins / Q000737:chemistry* / Q000235:genetics; D019281:Dimerization; D015870:Gene Expression; D005822:Genetic Vectors; D051379:Mice; D008970:Molecular Weight; D017433:Protein Structure, Secondary; D011994:Recombinant Proteins / Q000737:chemistry
chemical_list: D003459:Crystallins; D011994:Recombinant Proteins