Activation of inducible nitric oxide synthase by Yongdam-Sagan-Tang in mouse peritoneal macrophages.
The objective of the current study was to determine the effect of Yongdam-Sagan-Tang (YS-Tang) on the production of nitric oxide (NO). Stimulation of mouse peritoneal macrophages with YS-Tang after the treatment of recombinant interferon-gamma (rIFN-gamma) resulted in increased NO synthesis. YS-Tang had no effect on NO synthesis by itself. When YS-Tang was used in combination with rIFN-gamma, there was a marked co-operative induction of NO synthesis in a dose-dependent manner. The optimal effect of YS-Tang on NO synthesis was shown 6 h after treatment with rIFN-gamma. This increase in NO synthesis was reflected as an increased amount of inducible NO synthase (iNOS) protein. NO production was inhibited by NG-monomethyl-L-arginine. The increased production of NO from rIFN-gamma plus YS-Tang-stimulated cells was decreased by the treatment with staurosporin. In addition, synergy between rIFN-gamma and YS-Tang was mainly dependent on YS-Tang-induced tumor necrosis factor-alpha (TNF-alpha) secretion. All the preparations of YS-Tang were endotoxin free. These results suggest that the capacity of YS-Tang to increase NO production from rIFN-gamma-primed mouse peritoneal macrophages is the result of YS-Tang-induced TNF-alpha secretion.
Lee
E H
EH
Department of Oriental Pharmacy, College of Pharmacy, Wonkwang University, Iksan, Chonbuk, South Korea.
Kim
N K
NK
Hwang
C Y
CY
Kim
H M
HM
D000818:Animals; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D004789:Enzyme Activation; D007371:Interferon-gamma / Q000494:pharmacology; D017737:Macrophages, Peritoneal / Q000187:drug effects* / Q000201:enzymology; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D009569:Nitric Oxide / Q000096:biosynthesis; D019001:Nitric Oxide Synthase / Q000378:metabolism*; D052247:Nitric Oxide Synthase Type II; D011493:Protein Kinase C / Q000378:metabolism; D011994:Recombinant Proteins / Q000494:pharmacology; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism
D004365:Drugs, Chinese Herbal; D011994:Recombinant Proteins; D014409:Tumor Necrosis Factor-alpha; D009569:Nitric Oxide; D007371:Interferon-gamma; D019001:Nitric Oxide Synthase; D052247:Nitric Oxide Synthase Type II; C496317:Nos2 protein, mouse; D011493:Protein Kinase C