Carbons from choline present in the phospholipids of Pseudomonas aeruginosa.
The phospholipid composition of Pseudomonas aeruginosa grown in a mineral medium with choline as the carbon source was: phosphatidylethanolamine, 71.6 +/- 1.4%; phosphatidylglycerol, 11.8 +/- 0.4%; diphosphatidylglycerol, 0.8 +/- 0.4%; phosphatidic acid, 2.4 +/- 0.6%; lysophosphatidylethanolamine, 1.6 +/- 0.3%; phosphatidylcholine 7.9 +/- 0.3%; lysophosphatidylcholine, 3.9 +/- 0.7%. The molar ratio between the acidic and the neutral phospholipids was 0.18. Radiolabeling experiments with [methyl-14C]choline or [1,2-14C]choline carried out in cell suspension from bacteria that were grown in the presence of choline as the sole carbon source demonstrated that the carbons of the N-methyl groups of choline contributed to the synthesis of fatty acids while the carbons comprising the backbone of choline were used for the synthesis of glycerol.
Albelo
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Departamento de Biología Molecular, Universidad Nacional de Río Cuarto, Córdoba, Argentina.
Domenech
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D002244:Carbon / Q000378:metabolism*; D002250:Carbon Radioisotopes; D002794:Choline / Q000378:metabolism*; D010743:Phospholipids / Q000032:analysis* / Q000378:metabolism; D011550:Pseudomonas aeruginosa / Q000737:chemistry* / Q000378:metabolism*
D002250:Carbon Radioisotopes; D010743:Phospholipids; D002244:Carbon; D002794:Choline