Morphology and proliferation of B16 melanoma cells in the presence of lanthanoid and Al3+ ions.
The effects of trivalent metal ions such as lanthanoid (La3+, Ce3+, Nd3+, Sm3+, Gd3+, Er3+, Yb3+, Lu3+) and Al3+ ions on the morphological change and proliferation of B16 melanoma cells in culture are discussed. These metal ions induced morphological transformations and decreased growth rates at doses of 1 mM. B16 melanoma cells treated with La3+, Ce3+, Nd3+, Sm3+, and Gd3+ showed polyhedrical spreading. Elongation of axones was dependent on the metal ions. B16 melanoma cells treated with Er3+, Yb3+, Lu3+, and Al3+ showed a long slender shape. Growth rates of melanoma cells in the presence of 1 mM of metal ions (La3+, Ce3+, Nd3+, Sm3+, Gd3+, Yb3+, Al3+) were significantly lower than that of control cells. Measurements of cell cycle indicated that the metal ions arrested the transitions from G0/G1 to S state.
Sato
T
T
Department of Biomolecular Engineering, Tokyo Institute of Technology, Yokohama, Japan.
Hashizume
M
M
Hotta
Y
Y
Okahata
Y
Y
D000535:Aluminum / Q000494:pharmacology*; D000818:Animals; D002453:Cell Cycle / Q000187:drug effects; D002455:Cell Division / Q000187:drug effects; D048429:Cell Size / Q000187:drug effects; D003599:Cytoskeleton / Q000187:drug effects; D006367:HeLa Cells; D006801:Humans; D007700:Kinetics; D008546:Melanoma, Experimental / Q000188:drug therapy* / Q000473:pathology*; D008674:Metals, Rare Earth / Q000494:pharmacology*; D051379:Mice; D014407:Tumor Cells, Cultured
D008674:Metals, Rare Earth; D000535:Aluminum