Semi-preparative separation and purification of taxol analogs by high-speed countercurrent chromatography.
High-speed countercurrent chromatography (HSCCC) was applied to the semi-preparative separation of taxol and its analogs, such as cephalomannine and 7-epi-10-deacetyltaxol from the extract of the bark of Taxus yunnannesis. The experiments were performed with a quaternary two-phase solvent system composed of n-hexane-thyl acetate-ethanol-water through two steps. In the first step, the four components were separated into two groups at a volume ratio of 1:1:1:1 and, in the second step, two components in each group were separated at different volume ratios of 3:3:2:3 or 4:4:3:4. The present method also allows consecutive injections with reproducible results. HPLC analysis showed that the purity of the four components obtained from a partially purified sample, containing taxol at 10%, ranged from 85 to 99%. The results indicated that HSCCC can be effectively used for the semi-preparative separation and purification of taxol and its analogs.
Cao
X
X
Beijing Institute of New Technology Application, China.
Tian
Y
Y
Zhang
T Y
TY
Ito
Y
Y
D000972:Antineoplastic Agents, Phytogenic / Q000302:isolation & purification; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D003377:Countercurrent Distribution / Q000379:methods*; D017239:Paclitaxel / Q000031:analogs & derivatives / Q000302:isolation & purification*; D010944:Plants / Q000737:chemistry; D012997:Solvents / Q000737:chemistry; D043823:Taxoids
D000972:Antineoplastic Agents, Phytogenic; D012997:Solvents; D043823:Taxoids; C056348:cephalomannine; D017239:Paclitaxel