title: Muscarinic acetylcholine receptor subtypes in the human iris.
abstract: Employing subtype-specific antisera, we measured the relative immunoreactivity of five muscarinic acetylcholine receptor (mAChR) subtype proteins (m1-m5) in the human iris. The most intensive FITC immunofluorescence was detected by the anti-m3 antibody, followed by anti-m1 and -m5 antisera, in the iris sphincter muscle cells. Only very weak fluorescence was obtained by anti-m2 and -m4 antibodies. In dilator muscle cells, weak but not consistent immunoreactivity was found by anti-m1 and -m5 antibodies. The results suggest that the m3 muscarinic receptor is the predominant subtype in sphincter muscle cells of the human iris.
authors.affiliation: Department of Biophysics, Kanazawa University School of Medicine, Kanazawa 920-8640, Japan.
authors.forename: N
authors.initials: N
authors.lastname: Ishizaka
authors.forename: M
authors.initials: M
authors.lastname: Noda
authors.forename: S
authors.initials: S
authors.lastname: Yokoyama
authors.forename: K
authors.initials: K
authors.lastname: Kawasaki
authors.forename: M
authors.initials: M
authors.lastname: Yamamoto
authors.forename: H
authors.initials: H
authors.lastname: Higashida
mesh_terms: D000328:Adult; D005134:Eye Neoplasms / Q000601:surgery; D016650:Fluorescein-5-isothiocyanate; D006801:Humans; D007150:Immunohistochemistry; D066298:In Vitro Techniques; D007498:Iris / Q000378:metabolism*; D008545:Melanoma / Q000601:surgery; D008856:Microscopy, Fluorescence; D008875:Middle Aged; D011976:Receptors, Muscarinic / Q000378:metabolism*; D012025:Reflex, Pupillary / Q000502:physiology
chemical_list: D011976:Receptors, Muscarinic; D016650:Fluorescein-5-isothiocyanate