Cloning and characterization of mouse mSox13 cDNA.
A novel SRY-related cDNA, mSox13, was isolated from a lambda phage library derived from mouse embryo. The cDNA encodes a protein of 595 amino acids containing the SRY-type high mobility group (HMG) box and a putative leucine zipper motif. A sequence comparison of mSox13 and other type-D SOX proteins shows that the leucine zipper and a neighboring glutamine-rich sequence stretch, which was named Q box, are well conserved among known type-D SOX proteins. The expression of mSox13 is restricted to the kidney and ovary. The electrophoretic mobility shift assay indicates that the recombinant mSox13 protein is capable of binding to the AACAAT sequence.
Kido
S
S
Tokyo Electric Power Hospital, Japan.
Hiraoka
Y
Y
Ogawa
M
M
Sakai
Y
Y
Yoshimura
Y
Y
Aiso
S
S
D000595:Amino Acid Sequence; D000818:Animals; D001324:Autoantigens; D001483:Base Sequence; D001665:Binding Sites; D003001:Cloning, Molecular; D018076:DNA, Complementary; D004268:DNA-Binding Proteins / Q000737:chemistry; D004622:Embryo, Mammalian; D015723:Gene Library; D006609:High Mobility Group Proteins / Q000096:biosynthesis* / Q000737:chemistry; D016350:Leucine Zippers; D051379:Mice / Q000235:genetics*; D008969:Molecular Sequence Data; D009687:Nuclear Proteins; D009838:Oligodeoxyribonucleotides / Q000737:chemistry / Q000378:metabolism; D009928:Organ Specificity; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000737:chemistry; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid; D051876:Sex-Determining Region Y Protein; D013379:Substrate Specificity; D014157:Transcription Factors
D001324:Autoantigens; D018076:DNA, Complementary; D004268:DNA-Binding Proteins; D006609:High Mobility Group Proteins; D009687:Nuclear Proteins; D009838:Oligodeoxyribonucleotides; D011994:Recombinant Proteins; D051876:Sex-Determining Region Y Protein; C110455:Sox13 protein, mouse; C497746:Sry protein, mouse; D014157:Transcription Factors