title: Specific interactions between retrovirus Env and Gag proteins in rat neurons.
abstract: In this work we have studied the intracellular localization properties of the Gag and Env proteins of Moloney murine leukemia virus (MLV) and human immunodeficiency virus (HIV) in dorsal root ganglion (DRG) neurons of rat. These neurons form thick bundles of axons, which facilitates protein localization studies by immunofluorescence analyses. When such neuron cultures were infected with recombinant Semliki Forest virus particles carrying the gag genes of either retrovirus, the expressed Gag proteins were localized to both the somatic and the axonal regions of the DRG neurons. In contrast, the Env proteins were confined only to the somatic region. When the Gag and Env proteins were coexpressed, the Gag proteins were also excluded from the axons. This effect of the Env proteins was shown to be dependent on the concentration of the Gag proteins in the neuron and also to be specific for homologous pairs of retrovirus proteins. Therefore, the results suggest that there are specific interactions between the Env and the Gag proteins of MLV and HIV in the DRG neurons.
authors.affiliation: Department of Neuroscience, Karolinska Institute, Stockholm, Sweden.
authors.forename: K
authors.initials: K
authors.lastname: Weclewicz
authors.forename: M
authors.initials: M
authors.lastname: Ekström
authors.forename: K
authors.initials: K
authors.lastname: Kristensson
authors.forename: H
authors.initials: H
authors.lastname: Garoff
mesh_terms: D000818:Animals; D002460:Cell Line; D002478:Cells, Cultured; D006224:Cricetinae; D005260:Female; D005727:Ganglia, Spinal / Q000166:cytology / Q000378:metabolism; D015870:Gene Expression; D015686:Gene Products, env / Q000235:genetics / Q000378:metabolism*; D015683:Gene Products, gag / Q000235:genetics / Q000378:metabolism*; D006678:HIV / Q000235:genetics / Q000378:metabolism*; D006801:Humans; D051379:Mice; D008869:Microtubule-Associated Proteins / Q000032:analysis; D008979:Moloney murine leukemia virus / Q000235:genetics / Q000378:metabolism*; D009474:Neurons / Q000378:metabolism*; D011498:Protein Precursors / Q000032:analysis; D051381:Rats; D017207:Rats, Sprague-Dawley; D011990:Receptors, Transferrin / Q000032:analysis; D012672:Semliki forest virus; D014771:Virion
chemical_list: D015686:Gene Products, env; D015683:Gene Products, gag; D008869:Microtubule-Associated Proteins; D011498:Protein Precursors; D011990:Receptors, Transferrin