Aldosterone stimulates intestinal Na+ absorption in rats by increasing NHE3 expression of the proximal colon.
Na+ retention by the colon in response to salt deprivation is mediated in part by the resulting secondary hyperaldosteronism. We show that experimental hyperaldosteronism, to levels seen with salt deprivation, causes an increase in the selective expression and activity of NHE3, an apically located isoform of the Na+/H+ exchange family that functions in transepithelial Na+ absorption. The effect of aldosterone on NHE3 expression is tissue specific, occurring in intestine and not in kidney. Within the intestine, these effects are regional, being observed only in proximal colon, and different in distribution from that observed with glucocorticoids, where the predominant effect occurs in ileum. Although glucocorticoids are well known to exert many effects via regulation of transcript levels, the present study demonstrates that aldosterone stimulates intestinal Na+ absorption by increasing cellular NHE3 expression, a response that is tissue and region specific.
Cho
J H
JH
Department of Medicine, University of Chicago Hospitals, Illinois 60637, USA.
Musch
M W
MW
Bookstein
C M
CM
McSwine
R L
RL
Rabenau
K
K
Chang
E B
EB
D000042:Absorption; D000450:Aldosterone / Q000494:pharmacology*; D000818:Animals; D016764:Cell Polarity; D003106:Colon / Q000187:drug effects / Q000378:metabolism*; D006146:Guanidines / Q000494:pharmacology; D017403:In Situ Hybridization; D007668:Kidney / Q000187:drug effects / Q000378:metabolism; D008871:Microvilli / Q000187:drug effects; D008901:Mineralocorticoids / Q000172:deficiency / Q000378:metabolism; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D012964:Sodium / Q000493:pharmacokinetics*; D000074083:Sodium-Hydrogen Exchanger 3; D017923:Sodium-Hydrogen Exchangers / Q000235:genetics / Q000378:metabolism*; D013450:Sulfones / Q000494:pharmacology
D006146:Guanidines; D008901:Mineralocorticoids; D012333:RNA, Messenger; C513110:Slc9a2 protein, rat; C000613387:Slc9a3 protein, rat; D000074083:Sodium-Hydrogen Exchanger 3; D017923:Sodium-Hydrogen Exchangers; D013450:Sulfones; C074107:3-methylsulfonyl-4-piperidinobenzoyl guanidine; D000450:Aldosterone; D012964:Sodium