title: Synthesis of secretory component by rat hepatocytes in culture.
abstract: Normal rat hepatocytes were isolated and cultivated in vitro. Synthesis of secretory component was demonstrated by its accumulation in the culture medium, as measured by radioimmunoassay; by incorporation of 14C-leucine in the protein specifically precipitated with anti-secretory component antiserum; and by a positive precipitin reaction of concentrated culture medium with the same antiserum. The results explain the high levels of secretory component found in rat bile and render plausible a mechanism of hepatic IgA transfer involving secretory component as the hepatocyte membrane receptor for polymeric IgA.
authors.affiliation: Unit of Experimental Medicine, International Institute of Cellular and Molecular Pathology, Catholic University of Louvain, Brussels, Belgium.
authors.forename: J L
authors.initials: JL
authors.lastname: Zevenbergen
authors.forename: C
authors.initials: C
authors.lastname: May
authors.forename: J C
authors.initials: JC
authors.lastname: Wanson
authors.forename: J P
authors.initials: JP
authors.lastname: Vaerman
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D008099:Liver / Q000276:immunology* / Q000378:metabolism; D051381:Rats; D012635:Secretory Component / Q000096:biosynthesis*; D013997:Time Factors
chemical_list: D012635:Secretory Component