title: The TAATGARAT motif in the herpes simplex virus immediate-early gene promoters can confer both positive and negative responses to cellular octamer-binding proteins when it is located within the viral genome.
abstract: The TAATGARAT motif in the herpes simplex virus (HSV) immediate-early (IE) gene promoters plays a key role in their activation by the Oct-1-Vmw65 complex, but its role in mediating inhibitory effects of cellular octamer-binding proteins is less clear. We have used indicator viruses containing reporter constructs with different IE promoters driving a reporter beta-galactosidase gene within the viral genome to investigate this. We showed that deletion of the upstream IE promoter region containing the TAATGARAT motifs abolishes the inhibitory effect of the cellular octamer-binding proteins Oct-2.4 and Oct-2.5 on the viral IE promoter. This inhibitory effect can be restored by addition of a single TAATGARAT motif to the minimal promoter within the viral genome. Hence, the TAATGARAT motif can indeed mediate both positive and negative effects of cellular transcription factors when it is located within the viral genome.
authors.affiliation: Department of Molecular Pathology, Windeyer Institute of Medical Sciences, University College London Medical School, United Kingdom.
authors.forename: S
authors.initials: S
authors.lastname: Thomas
authors.forename: R S
authors.initials: RS
authors.lastname: Coffin
authors.forename: P
authors.initials: P
authors.lastname: Watts
authors.forename: G
authors.initials: G
authors.lastname: Gough
authors.forename: D S
authors.initials: DS
authors.lastname: Latchman
mesh_terms: D000818:Animals; D001665:Binding Sites; D002460:Cell Line; D006224:Cricetinae; D004268:DNA-Binding Proteins; D017930:Genes, Reporter; D016679:Genome, Viral; D018259:Herpesvirus 1, Human / Q000235:genetics* / Q000378:metabolism; D017874:Immediate-Early Proteins / Q000235:genetics* / Q000378:metabolism; D011401:Promoter Regions, Genetic; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism*; D001616:beta-Galactosidase / Q000235:genetics / Q000378:metabolism
chemical_list: D004268:DNA-Binding Proteins; C050678:IE1 protein, Human herpesvirus 1; D017874:Immediate-Early Proteins; D014157:Transcription Factors; D001616:beta-Galactosidase