Membrane targeting of RecA during genetic transformation.
Recombination in prokaryotes and eukaryotes is mediated by the RecA family of proteins. Although the interactions between RecA and DNA are well studied, the cellular location of these interactions is not known. Using genetic transformation of Streptococcus pneumoniae as a model system, there was increased expression of a protein, colligrin, and RecA, products of the rec locus during genetic transfer. These proteins formed a complex and were found associated with the membranes of genetically competent cells. With immunoelectron microscopy and subcellular fractionation, we showed that the induction of competence led to the translocation of RecA and colligrin to the membrane and to the formation of clusters of RecA in a colligrin-dependent step. Based on the behaviour of colligrin and RecA during genetic exchange and the numerous proteins in prokaryotes and eukaryotes with domains similar to colligrin, we suggest that there may exist a family of proteins, which gathers macromolecules at specific sites in biological membranes.
Masure
H R
HR
Department of Infectious Diseases, St Jude Children's Research Hospital, Memphis, TN 38105-2794, USA. robert.masure@stjude.org
Pearce
B J
BJ
Shio
H
H
Spellerberg
B
B
D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism*; D002462:Cell Membrane / Q000378:metabolism*; D015964:Gene Expression Regulation, Bacterial; D016253:Microscopy, Immunoelectron; D011938:Rec A Recombinases / Q000235:genetics / Q000378:metabolism*; D013296:Streptococcus pneumoniae / Q000235:genetics* / Q000378:metabolism; D014169:Transformation, Bacterial / Q000502:physiology*
D001426:Bacterial Proteins; C111750:colligrin protein, Streptococcus pneumoniae; D011938:Rec A Recombinases