Negative regulation of T cell homing by CD43.
We report that the cell surface mucin CD43 acts as an anti-adhesin on T lymphocytes. CD43-deficient murine lymphocytes homed significantly more frequently to secondary lymphoid organs than wild-type cells. Intravital microscopy of peripheral lymph node venules revealed that CD43-deficient lymphocytes were twice as likely to tether, roll, and stick than wild-type cells. This effect was due to CD43 interference with the homing receptor, L-selectin, and was most pronounced in venules with low L-selectin ligand density. In vitro, CD43-deficient cells tethered to L-selectin ligands more efficiently and rolled more slowly than wild-type lymphocytes. Thus, CD43 exerts a negative regulatory effect on T cell trafficking by counterbalancing L-selectin-mediated adhesion.
Stockton
B M
BM
Department of Pathology, Tufts University, Boston, Massachusetts 02111, USA.
Cheng
G
G
Manjunath
N
N
Ardman
B
B
von Andrian
U H
UH
D000818:Animals; D015703:Antigens, CD / Q000276:immunology*; D002448:Cell Adhesion; D005434:Flow Cytometry; D019041:L-Selectin / Q000276:immunology*; D051917:Leukosialin; D008198:Lymph Nodes; D051379:Mice; D008806:Mice, Inbred AKR; D008817:Mice, Mutant Strains; D018715:Microscopy, Video; D012795:Sialoglycoproteins / Q000172:deficiency / Q000276:immunology*; D013601:T-Lymphocytes / Q000276:immunology*
D015703:Antigens, CD; D051917:Leukosialin; D012795:Sialoglycoproteins; C497863:Spn protein, mouse; D019041:L-Selectin