Shotgun cloning and characterization of the thymidylate synthase-encoding gene from Mycobacterium bovis BCG.
The shotgun cloning of a Mycobacterium bovis BCG (BCG) genome into pBluescript SK (+) successfully yielded a 0.9 kbp fragment, confirming the ability of Escherichia coli thyA mutant MH2702 to grow in a thymine-depleted medium. This DNA fragment contained a gene homologous to the thymidylate synthase (TS)-encoding genes (thyA) of other organisms. An inverted repeat sequence and open reading frame (ORF) were observed at the upstream region of the thyA. A computer analysis revealed that the protein encoded by this ORF possessed a structure unique for a DNA binding protein.
Matsumoto
S
S
Nagasaki University School of Dentistry, Japan.
Yukitake
H
H
Ohara
N
N
Dairi
T
T
Kanbara
H
H
Yamada
T
T
D000595:Amino Acid Sequence; D001483:Base Sequence; D015139:Blotting, Southern; D015153:Blotting, Western; D003001:Cloning, Molecular; D004591:Electrophoresis, Polyacrylamide Gel; D015870:Gene Expression; D005798:Genes, Bacterial; D008969:Molecular Sequence Data; D009163:Mycobacterium bovis / Q000201:enzymology* / Q000235:genetics*; D012091:Repetitive Sequences, Nucleic Acid; D017422:Sequence Analysis, DNA; D013940:Thymidylate Synthase / Q000737:chemistry / Q000235:genetics*
D013940:Thymidylate Synthase