title: Immunohistochemical characterization of the cellular infiltrate in airway mucosa of toluene diisocyanate (TDI)-induced asthma: comparison with allergic asthma.
abstract: Toluene diisocyanate (TDI) is the most prevalent agent in occupational asthma (OA) in Korea. The immuno-pathologic mechanism for TDI-induced bronchoconstriction remains to be clarified. We studied the immunohistochemical finding of inflammatory cells in bronchial mucosa in subjects with TDI-induced asthma. Fiberoptic bronchial biopsy specimens were obtained from nine subjects with TDI-induced asthma. Six allergic asthma sensitive to house dust mite were enrolled as controls. Bronchial biopsy specimens were examined by immunohistology with a panel of monoclonal antibodies to mast cell tryptase (AA1), secretary form of eosinophil cationic protein (EG2), pan T-lymphocyte (CD3) and neutrophil elastase (NE). There was a significant increase in the number of AA1+, EG2+ and NE+ cells in TDI-induced asthma compared to those of allergic asthma (p=0.02, p=0.04, p=0.03, respectively). No significant differences were observed in the number of CD3+ cells (p=0.27). These findings support the view that neutrophil recruitment together with eosinophil and mast cell, may contribute to the bronchoconstriction induced by TDI.
authors.affiliation: Department of Allergy and Clinical Immunology, Ajou University School of Medicine, Suwon, Korea.
authors.forename: H S
authors.initials: HS
authors.lastname: Park
authors.forename: S C
authors.initials: SC
authors.lastname: Hwang
authors.forename: D H
authors.initials: DH
authors.lastname: Nahm
authors.forename: H E
authors.initials: HE
authors.lastname: Yim
mesh_terms: D000328:Adult; D001249:Asthma / Q000139:chemically induced / Q000276:immunology* / Q000473:pathology; D001980:Bronchi / Q000166:cytology / Q000276:immunology* / Q000473:pathology; D004804:Eosinophils / Q000166:cytology / Q000276:immunology; D005260:Female; D006801:Humans; D007958:Leukocyte Count; D008297:Male; D008407:Mast Cells / Q000166:cytology / Q000276:immunology; D008875:Middle Aged; D009092:Mucous Membrane / Q000276:immunology; D009504:Neutrophils / Q000166:cytology / Q000276:immunology; D013601:T-Lymphocytes / Q000166:cytology / Q000276:immunology; D014051:Toluene 2,4-Diisocyanate / Q000009:adverse effects*
chemical_list: D014051:Toluene 2,4-Diisocyanate