[Molecular cytogenetic study on four human esophageal cancer cell lines].
OBJECTIVE
To investigate the possible involvement of chromosome abnormalities in pathogenesis of human esophageal cancer.


METHODS
Four cell lines of human esophageal cancer (EC) established in our laboratory were analysed using interphase fluorescence in situ hybridization (FISH), chromosome painting technique and comparative genomic hybridization (CGH).


RESULTS
Chromosome gain of 1,2,3,8,16, 17, and 20 was found in the four cell lines, and loss of chromosome Y in cell line EC8712, EC8733 and EC8501 was noted. Other frequent changes were partial deletion of 1p, translocation of 2q and amplification of 5p in all 4 cell lines, and amplification of 8q and 13q in EC8733 and deletion of 17p in EC8712.


CONCLUSIONS
The data suggest that nonrandom chromosome aberrations may play an important role in the pathogenesis of human esophageal cancer.
Xiao
F
F
Nation Laboratory Molecular Oncology Cancer Institute, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100021 P. R. China.
Wang
X
X
Wang
M
M
Guan
X
X
Wu
M
M
D000328:Adult; D002460:Cell Line; D002869:Chromosome Aberrations; D004938:Esophageal Neoplasms / Q000235:genetics*; D005260:Female; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D008297:Male; D008875:Middle Aged; D014407:Tumor Cells, Cultured