title: Cloning of the gene for interstitial collagenase-3 (matrix metalloproteinase-13) from rabbit synovial fibroblasts: differential expression with collagenase-1 (matrix metalloproteinase-1).
abstract: Cartilage, bone and the interstitial stroma, composed largely of the interstitial collagens, types I, II and III, are remodelled by three members of the metalloproteinase (MMP) family, collagenase-1 (MMP-1), collagenase-2 (MMP-8) and collagenase-3 (MMP-13). MMP-1 and MMP-13 may contribute directly to disease progression, since they are induced in patients with rheumatoid arthritis and osteoarthritis. The study of MMP-1 and MMP-13 gene regulation in models of arthritic disease has been problematic because mice and rats, which are typically used, only possess a homologue of MMP-13. Here we show that in contrast with mice and rats, rabbits possess distinct genes homologous to human MMP-1 and MMP-13. Furthermore, rabbit MMP-13 is expressed simultaneously with MMP-1 in chondrocytes and synovial fibroblasts in response to the cytokines interleukin-1 and tumour necrosis factor-alpha, or the phorbol ester PMA. The time course of MMP-13 induction is more rapid and transient than that of MMP-1, suggesting that distinct mechanisms regulate the expression of these two collagenases. We have cloned the rabbit MMP-13 gene from synovial fibroblasts and demonstrated that the rabbit gene shares greater homology with human MMP-13 than does the mouse interstitial collagenase. Together with the fact that mice and rats do not possess a homologue to human MMP-1, our data suggest that the rabbit provides an appropriate model for studying the roles of interstitial collagenases in connective-tissue diseases, such as rheumatoid arthritis and osteoarthritis.
authors.affiliation: Department of Medicine, Dartmouth Medical School, Hanover, NH, 03755, USA. matthew.p.vincent@dartmouth.edu
authors.forename: M P
authors.initials: MP
authors.lastname: Vincenti
authors.forename: C I
authors.initials: CI
authors.lastname: Coon
authors.forename: J A
authors.initials: JA
authors.lastname: Mengshol
authors.forename: S
authors.initials: S
authors.lastname: Yocum
authors.forename: P
authors.initials: P
authors.lastname: Mitchell
authors.forename: C E
authors.initials: CE
authors.lastname: Brinckerhoff
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D017364:Collagenases / Q000235:genetics* / Q000378:metabolism*; D005347:Fibroblasts / Q000201:enzymology; D006801:Humans; D020781:Matrix Metalloproteinase 1; D053509:Matrix Metalloproteinase 13; D051379:Mice; D008969:Molecular Sequence Data; D011817:Rabbits; D051381:Rats; D016415:Sequence Alignment; D013583:Synovial Membrane / Q000201:enzymology*
chemical_list: D017364:Collagenases; C507079:MMP13 protein, human; D053509:Matrix Metalloproteinase 13; C507081:Mmp13 protein, mouse; C507083:Mmp13 protein, rat; D020781:Matrix Metalloproteinase 1