title: Antioxidants inhibit the in vitro production of inflammatory cytokines in Crohn's disease and ulcerative colitis.
abstract: BACKGROUND
  Modulation of cytokine secretion may be of interest in the treatment of Crohn's disease or ulcerative colitis.
  
  
  METHODS
  The effect of three antioxidants - butylated hydroxyanisol, tetrahydropapaveroline and nordihydroguaiaretic acid - on the production of tumour necrosis factor (TNF), interleukin (IL) 1, IL-6 and IL-8 (measured by enzyme-linked immunosorbent assay) by peripheral mononuclear cells and biopsies of inflamed colonic mucosa from inflammatory bowel disease patients were studied.
  
  
  RESULTS
  We observed a decrease in IL-1 and IL-6 production by peripheral mononuclear cells from inflammatory bowel disease patients (approximately 50% of control). The three drugs did not decrease IL-6 and IL-8 secretion by colonic biopsies, whereas they did inhibit IL-1 and, to some degree, TNF production. The cytokine-inhibitory effect of antioxidants seems to be more pronounced in ulcerative colitis than in Crohn's disease.
  
  
  CONCLUSIONS
  Our results suggest that the studied antioxidants, or related compounds, may be of interest in inflammatory bowel disease treatment.
authors.affiliation: Centre de Recherches Pharmacologiques, Illkirch-Graffenstaden, France.
authors.forename: J M
authors.initials: JM
authors.lastname: Reimund
authors.forename: A C
authors.initials: AC
authors.lastname: Allison
authors.forename: C D
authors.initials: CD
authors.lastname: Muller
authors.forename: S
authors.initials: S
authors.lastname: Dumont
authors.forename: J S
authors.initials: JS
authors.lastname: Kenney
authors.forename: R
authors.initials: R
authors.lastname: Baumann
authors.forename: B
authors.initials: B
authors.lastname: Duclos
authors.forename: P
authors.initials: P
authors.lastname: Poindron
mesh_terms: D000328:Adult; D000975:Antioxidants / Q000494:pharmacology*; D002083:Butylated Hydroxyanisole / Q000494:pharmacology; D002474:Cell-Free System / Q000378:metabolism; D002478:Cells, Cultured; D003093:Colitis, Ulcerative / Q000378:metabolism*; D003424:Crohn Disease / Q000378:metabolism*; D016207:Cytokines / Q000037:antagonists & inhibitors* / Q000096:biosynthesis*; D004121:Dimethyl Sulfoxide / Q000494:pharmacology; D006801:Humans; D007413:Intestinal Mucosa / Q000378:metabolism; D007963:Leukocytes, Mononuclear / Q000378:metabolism; D009637:Masoprocol / Q000494:pharmacology; D008875:Middle Aged; D009924:Organ Culture Techniques
chemical_list: D000975:Antioxidants; D016207:Cytokines; D002083:Butylated Hydroxyanisole; D009637:Masoprocol; D004121:Dimethyl Sulfoxide