A novel epitope tag for the detection of rabGTPases.
Rab GTPases are localized on the cytoplasmic surface of most intracellular organelles where they play a role in the regulation of vesicular transport. As it has been difficult to detect endogenous rab proteins by morphological methods, their localizations were often inferred from transfection experiments using epitope-tagged constructs. Because most of the available epitope tags are only recognitzed by mouse monoclonal antibodies they are often not suitable for double or triple label immunocytochemistry. To overcome this problem, we generated antibodies against a novel 10 amino acid X31 influenza hemagglutin epitope (NH). We here characterized these antibodies and document their utility for detecting early endosomal rab proteins N-terminally tagged with the NH decapeptide in morphological and biochemical assays.
Nagelkerken
B
B
Department of Cell Biology, Utrecht University School of Medicine, The Netherlands.
Mohrmann
K
K
Gerez
L
L
van Raak
M
M
Leijendekker
R
R
van der Sluijs
P
P
D000818:Animals; D001483:Base Sequence; D016466:CHO Cells; D006224:Cricetinae; D017931:DNA Primers; D018985:Epitopes, B-Lymphocyte / Q000276:immunology*; D019204:GTP-Binding Proteins / Q000032:analysis* / Q000235:genetics / Q000276:immunology; D006150:Guanine Nucleotides; D006168:Guinea Pigs; D006367:HeLa Cells; D019267:Hemagglutinin Glycoproteins, Influenza Virus / Q000276:immunology*; D006801:Humans; D051379:Mice; D008969:Molecular Sequence Data; D009154:Mutation; D011817:Rabbits; D020695:rab4 GTP-Binding Proteins; D020696:rab5 GTP-Binding Proteins
D017931:DNA Primers; D018985:Epitopes, B-Lymphocyte; D006150:Guanine Nucleotides; D019267:Hemagglutinin Glycoproteins, Influenza Virus; D019204:GTP-Binding Proteins; D020695:rab4 GTP-Binding Proteins; D020696:rab5 GTP-Binding Proteins