Probing the yeast phase-specific expression of the CBP1 gene in Histoplasma capsulatum.
Histoplasma capsulatum is a pathogenic fungus that exists in two distinct forms. The saprophytic mycelial phase inhabits moist soil environments; once inhaled, hyphae and conidia convert to a unicellular yeast phase that is capable of parasitizing macrophage phagolysosomes. Yeasts cultures, but not mycelial cultures, release large quantities of a calcium-binding protein (CBP) which may be important in calcium acquisition during intracellular parasitism. In this study, we show that the gene encoding CBP (CBP1) is transcriptionally regulated. To identify promoter sequences that are important for yeast phase-specific activity, we created a series of fusions between successively truncated CBP1 5' untranslated regulatory sequences and the Escherichia coli lacZ gene. The fusions were constructed on a telomeric shuttle plasmid that can replicate autonomously in the fungus. By assaying for beta-galactosidase activity from H. capsulatum transformants, we identified a 102-bp region that mediates promoter activation and yeast phase promoter activity. Base pair substitution analysis suggests that the sequences between 839 and 877 bp upstream of the start codon are the most important for this positive regulation.
Patel
J B
JB
Department of Molecular Microbiology, Washington University, St. Louis, Missouri 63110, USA.
Batanghari
J W
JW
Goldman
W E
WE
D001483:Base Sequence; D002135:Calcium-Binding Proteins / Q000235:genetics*; D015966:Gene Expression Regulation, Fungal; D006658:Histoplasma / Q000235:genetics*; D008969:Molecular Sequence Data; D010957:Plasmids; D011401:Promoter Regions, Genetic; D016615:Telomere; D014158:Transcription, Genetic
D002135:Calcium-Binding Proteins