Muscarinic acetylcholine receptor subtypes in the human iris.
Employing subtype-specific antisera, we measured the relative immunoreactivity of five muscarinic acetylcholine receptor (mAChR) subtype proteins (m1-m5) in the human iris. The most intensive FITC immunofluorescence was detected by the anti-m3 antibody, followed by anti-m1 and -m5 antisera, in the iris sphincter muscle cells. Only very weak fluorescence was obtained by anti-m2 and -m4 antibodies. In dilator muscle cells, weak but not consistent immunoreactivity was found by anti-m1 and -m5 antibodies. The results suggest that the m3 muscarinic receptor is the predominant subtype in sphincter muscle cells of the human iris.
Ishizaka
N
N
Department of Biophysics, Kanazawa University School of Medicine, Kanazawa 920-8640, Japan.
Noda
M
M
Yokoyama
S
S
Kawasaki
K
K
Yamamoto
M
M
Higashida
H
H
D000328:Adult; D005134:Eye Neoplasms / Q000601:surgery; D016650:Fluorescein-5-isothiocyanate; D006801:Humans; D007150:Immunohistochemistry; D066298:In Vitro Techniques; D007498:Iris / Q000378:metabolism*; D008545:Melanoma / Q000601:surgery; D008856:Microscopy, Fluorescence; D008875:Middle Aged; D011976:Receptors, Muscarinic / Q000378:metabolism*; D012025:Reflex, Pupillary / Q000502:physiology
D011976:Receptors, Muscarinic; D016650:Fluorescein-5-isothiocyanate