title: Construction and characterization of type 1 non-fimbriate and non-adhesive mutants of Salmonella typhimurium.
abstract: Mutations in the fimH gene of Salmonella typhimurium result in a non-fimbriate, non-adhesive phenotype. This phenotype was shown to be due to the lack of both fimH and fimF expression since disruption of the fimH gene by insertion of a DNA cassette into this determinant results in mutants that are complemented by plasmids carrying both fimH and fimF. Deletion mutations within the S. typhimurium fimH gene carried on a recombinant plasmid can be used to complement the mutant, and these transformants are non-adhesive but fully fimbriate, consistent with the role of FimH as being necessary for fimbrial adhesin expression. Adherence to erythrocytes, HeLa, and Hep-2 cells is associated with expression of the FimH polypeptide, and fimbriate strains that cannot synthesize FimH are non-adhesive. Discrete differences in the amino acid sequences of the adhesive type 1 and the non-hemagglutinating type 2 FimH polypeptides were detected, and are most likely responsible for the differences in hemagglutinating activity.
authors.affiliation: Department of Microbiology, University of Iowa, College of Medicine, Iowa City 52242, USA.
authors.forename: L S
authors.initials: LS
authors.lastname: Hancox
authors.forename: K S
authors.initials: KS
authors.lastname: Yeh
authors.forename: S
authors.initials: S
authors.lastname: Clegg
mesh_terms: D018829:Adhesins, Bacterial / Q000096:biosynthesis / Q000737:chemistry / Q000235:genetics*; D018830:Adhesins, Escherichia coli; D000595:Amino Acid Sequence; D001422:Bacterial Adhesion / Q000235:genetics* / Q000502:physiology; D001483:Base Sequence; D015139:Blotting, Southern; D002460:Cell Line; D004269:DNA, Bacterial / Q000737:chemistry; D036781:Fimbriae Proteins; D010861:Fimbriae, Bacterial / Q000737:chemistry*; D015964:Gene Expression Regulation, Bacterial; D006367:HeLa Cells; D006386:Hemagglutination Tests; D006801:Humans; D008969:Molecular Sequence Data; D005810:Multigene Family / Q000235:genetics; D016254:Mutagenesis, Insertional; D010641:Phenotype; D016133:Polymerase Chain Reaction; D011993:Recombinant Fusion Proteins / Q000737:chemistry; D012486:Salmonella typhimurium / Q000235:genetics* / Q000648:ultrastructure; D017422:Sequence Analysis, DNA
chemical_list: D018829:Adhesins, Bacterial; D018830:Adhesins, Escherichia coli; D004269:DNA, Bacterial; D011993:Recombinant Fusion Proteins; C058769:fimH protein, E coli; D036781:Fimbriae Proteins