Nonlinear ribosome migration on cauliflower mosaic virus 35S RNA in transgenic tobacco plants.
Cauliflower mosaic virus (CaMV) uses a specialised translation mechanism to bypass the long leader sequence of the 35S RNA. The effect of the CaMV 35S RNA leader sequence on the expression of a downstream beta-glucuronidase (GUS) reporter gene was studied in transgenic tobacco plants. Enzymatic GUS assays of these transgenic plants show that a shunt mechanism of translation indeed occurs in planta with an average efficiency of 5% compared with the leaderless construct. Histological GUS analyses indicate that the shunt mechanism occurs throughout the whole plant and at all developmental stages.
Schärer-Hernández
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N
Friedrich Miescher-Institut, Basel, Switzerland.
Hohn
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T
D015139:Blotting, Southern; D017796:Caulimovirus / Q000235:genetics*; D005966:Glucuronidase / Q000378:metabolism; D006651:Histocytochemistry; D030821:Plants, Genetically Modified / Q000235:genetics / Q000378:metabolism*; D010947:Plants, Toxic; D014176:Protein Biosynthesis; D021382:Protein Sorting Signals; D012333:RNA, Messenger / Q000502:physiology; D012367:RNA, Viral / Q000502:physiology*; D012270:Ribosomes / Q000502:physiology*; D014026:Nicotiana / Q000235:genetics / Q000378:metabolism*
D021382:Protein Sorting Signals; D012333:RNA, Messenger; D012367:RNA, Viral; D005966:Glucuronidase