Glutamylation of centriole and cytoplasmic tubulin in proliferating non-neuronal cells.
We have examined the distribution of glutamylated tubulin in non-neuronal cell lines. A major part of centriole tubulin is highly modified on both the alpha- and beta-tubulin subunits, whereas a minor part of the cytoplasmic tubulin is slightly modified, on the beta-tubulin only. Furthermore, we observed that tubulin glutamylation varies during the cell cycle: an increase occurs during mitosis on both centriole and spindle microtubules. In the spindle, this increase appears more obvious on the pole-to-pole and kinetochore microtubules than on the astral microtubules. The cellular pattern and the temporal variation of this post-translational modification contrast with other previously described tubulin modifications. The functional significance of this distribution is discussed.
Bobinnec
Y
Y
Institut Curie, section Recherche, UMR144 du CNRS, Paris, France.
Moudjou
M
M
Fouquet
J P
JP
Desbruyères
E
E
Eddé
B
B
Bornens
M
M
D000818:Animals; D002460:Cell Line; D002502:Centrioles / Q000737:chemistry*; D003593:Cytoplasm / Q000737:chemistry*; D006801:Humans; D018386:Kinetochores / Q000737:chemistry; D008221:Lymphoid Tissue; D051379:Mice; D008870:Microtubules / Q000737:chemistry; D008938:Mitosis; D011099:Polyglutamic Acid / Q000032:analysis*; D008941:Spindle Apparatus / Q000737:chemistry; D014404:Tubulin / Q000032:analysis*
D014404:Tubulin; D011099:Polyglutamic Acid