Cerebral vessels express interleukin 1beta after focal cerebral ischemia.
Rapid and marked increased levels of expression of interleukin 1beta (IL-1beta) mRNA have been detected in animal models of cerebral ischemia. However, the protein production of IL-1beta and the cellular sources of IL-1beta are largely undefined after cerebral ischemia. In the present study, we have measured the cellular localization of IL-1beta protein in brain tissue from non-ischemic and ischemic mice using immunohistochemistry. Male C57B/6J (n=45) mice were subjected to middle cerebral artery (MCA) occlusion by a clot or a suture. The mice were sacrificed at time points spanning the period from 15 min to 24 h after onset of the MCA occlusion. Non-operated and sham-operated mice were used as control groups. A monoclonal anti-IL-1beta antibody was used to detect IL-1beta. In the non-operated and sham-operated mice, a few IL-1beta immunoreactive cells were detected scattered throughout both hemispheres. IL-1beta immunoreactive cells increased in the ischemic lesion as early as 15 min and peaked at 1 h to 2 h after MCA occlusion. IL-1beta immunoreactivity was detected in the cortex of the contralateral hemisphere 1 h after ischemia. By 24 h after onset of ischemia, IL-1beta immunoreactivity was mainly present adjacent to the ischemic lesion and in the non-ischemic cortex. IL-1beta immunoreactivity was found on endothelial cells and microglia. This study demonstrates an early bilateral expression of IL-1beta on endothelium after MCA occlusion in mice.
Zhang
Z
Z
Henry Ford Health Sciences Center, Department of Neurology, Detroit, MI 48202, USA.
Chopp
M
M
Goussev
A
A
Powers
C
C
D000818:Animals; D000911:Antibodies, Monoclonal; D002536:Cerebral Arteries / Q000378:metabolism*; D004730:Endothelium, Vascular / Q000378:metabolism; D007150:Immunohistochemistry; D007375:Interleukin-1 / Q000096:biosynthesis*; D002542:Intracranial Embolism and Thrombosis / Q000378:metabolism; D002546:Ischemic Attack, Transient / Q000378:metabolism*; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D013537:Sutures
D000911:Antibodies, Monoclonal; D007375:Interleukin-1