title: Analysis of the G93E mutant allele of KpsM, the membrane component of an ABC transporter involved in polysialic acid translocation in Escherichia coli K1.
abstract: KpsM is an integral membrane protein involved in the translocation of the polysialic acid capsule of Escherichia coli K1. The kpsMG93E allele is a point mutation in the first cytoplasmic loop (Cl) of KpsM which partially disrupts translocation of the capsule. While producing polymer of wild-type length, strains harboring the G93E allele exhibit a decreased production of capsular polymer and a reduced rate of polymer translocation to the cell surface.
authors.affiliation: Department of Microbiology and Immunology, University of Rochester Medical Center, NY 14642, USA.
authors.forename: R P
authors.initials: RP
authors.lastname: Pigeon
authors.forename: R P
authors.initials: RP
authors.lastname: Silver
mesh_terms: D018528:ATP-Binding Cassette Transporters / Q000235:genetics*; D000483:Alleles; D000942:Antigens, Bacterial; D016667:Bacterial Capsules / Q000737:chemistry / Q000378:metabolism; D001692:Biological Transport / Q000235:genetics; D002462:Cell Membrane / Q000737:chemistry / Q000378:metabolism; D004926:Escherichia coli / Q000235:genetics* / Q000378:metabolism; D009154:Mutation; D011134:Polysaccharides / Q000378:metabolism; D011135:Polysaccharides, Bacterial / Q000235:genetics* / Q000378:metabolism; D012794:Sialic Acids / Q000378:metabolism*
chemical_list: D018528:ATP-Binding Cassette Transporters; D000942:Antigens, Bacterial; D011134:Polysaccharides; D011135:Polysaccharides, Bacterial; D012794:Sialic Acids; C036962:capsular polysaccharide K1; C021319:polysialic acid