Infrequent inactivation of DCC gene in replication error-positive colorectal cancers.
Colorectal cancers with and without the replication error (RER) exhibit fundamental differences in genotype and phenotype. While alterations in APC, p53, and K-ras genes have been characterized between RER+ and RER- colorectal cancers, the status of deleted in colorectal carcinomas (DCC) gene has not been yet. Alterations of DCC gene were analyzed in stage-matched two panels of 30 RER+ and 30 RER- colorectal cancers using semiquantitative reverse transcription-PCR and PCR-LOH analyses. Loss or reduction of DCC mRNA expression and allelic loss at the DCC locus were significantly less frequent in RER+ cancers than in RER- cancers. Interestingly, reduced DCC mRNA expression was observed in all 5 RER- cancers with liver metastasis. Our results support the concept that RER+ and RER- colorectal cancers represent different pathways of carcinogenesis and may give a hint for clarifying the specific mechanism of DCC inactivation in RER- colorectal cancers.
Yamamoto
H
H
First Department of Internal Medicine, Sapporo Medical University, Japan.
Itoh
F
F
Kusano
M
M
Yoshida
Y
Y
Hinoda
Y
Y
Imai
K
K
D015179:Colorectal Neoplasms / Q000235:genetics* / Q000473:pathology; D004251:DNA Transposable Elements; D015972:Gene Expression Regulation, Neoplastic; D016163:Genes, DCC; D006801:Humans; D019656:Loss of Heterozygosity; D018895:Microsatellite Repeats; D009367:Neoplasm Staging; D016133:Polymerase Chain Reaction / Q000592:standards; D012333:RNA, Messenger / Q000096:biosynthesis; D012334:RNA, Neoplasm / Q000096:biosynthesis; D012015:Reference Standards; D017384:Sequence Deletion
D004251:DNA Transposable Elements; D012333:RNA, Messenger; D012334:RNA, Neoplasm