A tRNA(Glu) gene from the hyperthermophilic archaeon Pyrococcus furiosus contains the 3'-terminal CCA sequence of the mature tRNA.
We cloned a gene encoding tRNA(Glu) of the hyperthermophilic archaeon Pyrococcus furiosus. This gene contains the CCA sequence corresponding to the 3'-terminus of the mature tRNA. It is known that, like in eukaryal tRNAs, the CCA-termini of archaeal tRNAs are generally not encoded. Therefore, we analyzed all tRNA genes in the genome of Methanococcus jannaschii estimated by its whole genome sequence. Twenty-one of 37 listed tRNA genes contained the 3'-terminal CCA sequence. The corresponding M. jannaschii tRNA(Glu) gene does not contain the CCA sequence, although the tRNA sequences of the M. jannaschii and P. furiosus tRNA(Glu) genes are 86% identical.
Cann
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Department of Molecular Biology, Biomolecular Engineering Research Institute, Osaka, Japan.
Ishino
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Y
D000926:Anticodon; D001105:Archaea / Q000235:genetics; D001483:Base Sequence; D003001:Cloning, Molecular; D019766:Genes, Archaeal; D017017:Methanococcus / Q000235:genetics*; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation; D019711:Pyrococcus / Q000235:genetics*; D012316:RNA Nucleotidyltransferases; D019642:RNA, Archaeal / Q000737:chemistry / Q000235:genetics; D012352:RNA, Transfer, Glu / Q000737:chemistry / Q000235:genetics*; D017422:Sequence Analysis, DNA
D000926:Anticodon; D019642:RNA, Archaeal; D012352:RNA, Transfer, Glu; D012316:RNA Nucleotidyltransferases