[The isolation and subtyping of the human immunodeficiency virus (HIV) from children in Moldova. The evidence for subtype F].
HIV epidemic related to nosocomial transmission in Romanian nursed children represents up to now more than 50% of paediatric AIDS cases in Europe. Although the sources of this epidemic are obscure, HIV-1 subtype F, a minor form in Brasil and Africa, realised a founder effect in this risk group, thus becoming the major form in Romanian epidemic. This study presents the results of the virus isolation from HIV-infected children in Northeast Romania. Heteroduplex mobility assay (HMA) was performed in order to establish HIV-1 viral subtype for 6 isolates. All tested HIV-1 strains were proved to belong to F subtype. So, our study confirm that HIV-1 subtype F is responsible for the epidemic in the risk group of Romanian nosocomially infected children.
Apetrei
C
C
Laboratorul de Virologie, Universitatea de Medicină şi Farmacie Gr. T. Popa, Iaşi.
Collin
G
G
Diculencu
D
D
Ajaka
I L
IL
Farfara
I
I
Pânzaru
C
C
Coman
G
G
Duca
M
M
Brun-Vezinet
F
F
Simon
F
F
D000163:Acquired Immunodeficiency Syndrome / Q000821:virology; D001483:Base Sequence; D002648:Child; D017931:DNA Primers; D004279:DNA, Viral / Q000235:genetics; D005838:Genotype; D015658:HIV Infections / Q000821:virology; D006679:HIV Seropositivity / Q000821:virology; D015497:HIV-1 / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D006801:Humans; D008966:Moldova; D008969:Molecular Sequence Data; D009692:Nucleic Acid Heteroduplexes / Q000235:genetics; D014776:Virus Cultivation / Q000379:methods
D017931:DNA Primers; D004279:DNA, Viral; D009692:Nucleic Acid Heteroduplexes
Izolarea şi subtiparea virusului imunodeficienţei umane (HIV) la copii din Moldova. Evidenţierea subtipului F.