Immunodetection of the murine chemotactic protein CP-10 in bleomycin-induced pulmonary injury.
The murine S-100 protein designated CP-10 is a potent chemotactic factor for phagocytic cells, exhibiting optimal activity in the picomolar range. We assessed the role of this cytokine in the inflammatory response to pulmonary injury following intratracheal administration of bleomycin to mice. In the lungs of normal animals, strong cytoplasmic immunostaining for CP-10 was demonstrable in all recognisable neutrophil leucocytes sequestered within alveolar capillaries. Following induction of pulmonary inflammation in susceptible C57BL/6 mice, numerous CP-10-positive neutrophils were observed, but many of the recruited neutrophils did not exhibit staining for CP-10. No other cells were immunoreactive. The concentration of CP-10 in bronchoalveolar lavage (BAL) fluids from normal mice and mice administered intratracheal saline was below the level of detection by enzyme immunoassay. In contrast, nanomolar levels of CP-10 were detected in unconcentrated BAL fluids from C57BL/6 mice after bleomycin-induced injury, and the presence of monomeric CP-10 was demonstrable by Western blotting. Elevation of CP-10 levels correlated with the influx of inflammatory cells in C57BL/6 mice, but was not demonstrable in BAL fluids from BALB/c mice, which are resistant to pulmonary injury by bleomycin. We conclude that CP-10 may contribute to the recruitment of inflammatory cells in bleomycin-induced lung damage.
Kumar
R K
RK
School of Pathology, University of New South Wales, Australia.
Harrison
C A
CA
Cornish
C J
CJ
Kocher
M
M
Geczy
C L
CL
D000818:Animals; D001761:Bleomycin; D015153:Blotting, Western; D001992:Bronchoalveolar Lavage Fluid / Q000737:chemistry / Q000166:cytology; D002135:Calcium-Binding Proteins / Q000032:analysis*; D040501:Calgranulin A; D002196:Capillaries / Q000737:chemistry / Q000166:cytology; D002630:Chemotactic Factors / Q000032:analysis; D005260:Female; D007118:Immunoassay; D007150:Immunohistochemistry; D007963:Leukocytes, Mononuclear / Q000737:chemistry / Q000166:cytology; D008168:Lung / Q000737:chemistry* / Q000166:cytology; D008171:Lung Diseases / Q000139:chemically induced / Q000378:metabolism*; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D009504:Neutrophils / Q000737:chemistry / Q000166:cytology; D011650:Pulmonary Alveoli / Q000098:blood supply / Q000737:chemistry / Q000166:cytology; D009418:S100 Proteins; D013047:Specific Pathogen-Free Organisms
D002135:Calcium-Binding Proteins; D040501:Calgranulin A; D002630:Chemotactic Factors; D009418:S100 Proteins; C086145:S100a8 protein, mouse; D001761:Bleomycin