title: Sensitivity- and gradient-enhanced hetero (omega1) half-filtered TOCSY experiment for measuring long-range heteronuclear coupling constants.
abstract: An enhanced version of the X (omega1) half-filtered TOCSY experiment for measurement of long-range heteronuclear coupling constants is proposed which yields high-quality spectra with substantially increased sensitivity and resolution. The modified method features gradient-enhanced X filtering sequences, broadband homonuclear decoupling during t1, optional 1JXH scaling in the F1 domain, and gradient coherence selection in combination with the sensitivity-enhanced protocol for the TOCSY transfer. These modifications extend the applicability of the method--coupling constants can be measured accurately for natural abundance samples at low concentrations and for compounds yielding complex spectra. Computer-aided analysis of E.COSY-type multiplets is applied for the determination of heteronuclear long-range coupling constants.
authors.affiliation: Department of Chemistry, University of Edinburgh, United Kingdom.
authors.forename: D
authors.initials: D
authors.lastname: Uhrín
authors.forename: G
authors.initials: G
authors.lastname: Batta
authors.forename: V J
authors.initials: VJ
authors.lastname: Hruby
authors.forename: P N
authors.initials: PN
authors.lastname: Barlow
authors.forename: K E
authors.initials: KE
authors.lastname: Kövér
mesh_terms: D009682:Magnetic Resonance Spectroscopy / Q000379:methods*; D009842:Oligopeptides / Q000737:chemistry*; D012680:Sensitivity and Specificity; D014312:Trisaccharides / Q000737:chemistry*
chemical_list: D009842:Oligopeptides; D014312:Trisaccharides