title: A novel plasmid pIJB1 possessing a putative 2,4-dichlorophenoxyacetate degradative transposon Tn5530 in Burkholderia cepacia strain 2a.
abstract: A 102-kb plasmid, pIJB1, was isolated from Burkholderia cepacia strain 2a, which is able to use 2,4-dichlorophenoxyacetate (2,4-D) as a sole carbon source, and a physical map of the plasmid has been established. It was observed that spontaneous loss of a 37-kb fragment of the plasmid after growth in nonselective medium occurred, generating a plasmid of diminished size, pIJB2. The deletion event is concomitant with the loss of the 2,4-D dissimilatory phenotype, indicating that at least some of the 2,4-D degradative genes are on the missing fragment. The missing fragment is flanked by two identical 4.3-kb insertion sequences (IS) and shows a typical composite transposon structure of 41-kb in size, designated Tn5530. The mutant plasmid pIJB2 possesses a single copy of the IS element.
authors.affiliation: School of Chemical and Life Sciences, University of Greenwich, London, United Kingdom.
authors.forename: X S
authors.initials: XS
authors.lastname: Xia
authors.forename: S
authors.initials: S
authors.lastname: Aathithan
authors.forename: K
authors.initials: K
authors.lastname: Oswiecimska
authors.forename: A R
authors.initials: AR
authors.lastname: Smith
authors.forename: I J
authors.initials: IJ
authors.lastname: Bruce
mesh_terms: D015084:2,4-Dichlorophenoxyacetic Acid / Q000378:metabolism*; D001673:Biodegradation, Environmental; D016956:Burkholderia cepacia / Q000235:genetics* / Q000378:metabolism*; D004251:DNA Transposable Elements / Q000235:genetics*; D018628:Gene Dosage; D006540:Herbicides / Q000378:metabolism; D010641:Phenotype; D010957:Plasmids / Q000235:genetics*; D015183:Restriction Mapping
chemical_list: D004251:DNA Transposable Elements; D006540:Herbicides; D015084:2,4-Dichlorophenoxyacetic Acid