title: Conserved enzyme-substrate electrostatic attraction in prokaryotic Cu,Zn superoxide dismutases.
abstract: The catalytic activity of wild type Escherichia coli Cu,Zn superoxide dismutases and of two mutants in which two lysine residues conserved in most bacterial Cu,Zn superoxide dismutases have been replaced by serine was investigated by pulse radiolysis and Brownian dynamics simulations. Experimental and computational data show that neutralization of Lys60 strongly reduces the catalytic activity of the enzyme (approximately 50%), indicating that this residue has a primary role in the electrostatic attraction of the substrate towards the catalytic copper. Neutralization of Lys63 does not significantly influence the catalytic rate constant. The results suggest that prokaryotic Cu,Zn superoxide dismutases have evolved an electrostatic mechanism to facilitate the enzyme-substrate encounter that is functionally equivalent to that already found in the eukaryotic enzymes.
authors.affiliation: Department of Biology, University of Rome Tor Vergata, Italy.
authors.forename: S
authors.initials: S
authors.lastname: Folcarelli
authors.forename: A
authors.initials: A
authors.lastname: Battistoni
authors.forename: M
authors.initials: M
authors.lastname: Falconi
authors.forename: P
authors.initials: P
authors.lastname: O'Neill
authors.forename: G
authors.initials: G
authors.lastname: Rotilio
authors.forename: A
authors.initials: A
authors.lastname: Desideri
mesh_terms: D000595:Amino Acid Sequence; D001665:Binding Sites; D017124:Conserved Sequence; D003300:Copper; D004926:Escherichia coli / Q000201:enzymology*; D008239:Lysine / Q000235:genetics; D008958:Models, Molecular; D008969:Molecular Sequence Data; D016297:Mutagenesis, Site-Directed; D011387:Prokaryotic Cells; D011675:Pulse Radiolysis; D017386:Sequence Homology, Amino Acid; D055672:Static Electricity; D013482:Superoxide Dismutase / Q000378:metabolism*; D013481:Superoxides / Q000378:metabolism
chemical_list: D013481:Superoxides; D003300:Copper; D013482:Superoxide Dismutase; D008239:Lysine