Nitric oxide (NO) disrupts specific DNA binding of the transcription factor c-Myb in vitro.
In an attempt to elucidate signal transduction pathways which may modulate DNA binding of the transcription factor c-Myb, we investigated whether c-Myb could be a target for the signaling molecule nitric oxide (NO) in vitro. NO-generating agents severely inhibited specific DNA binding of the c-Myb minimal DNA-binding domain R2R3. This inhibition was readily reversible upon treatment with excess DTT. A redox-sensitive cysteine (C130) was required for this NO sensitivity. Moreover, a DNA-binding domain carrying two of the avian myeloblastosis virus (AMV)-specific mutations (L106H, V117D) appeared to be less sensitive to S-nitrosylation than the wild-type c-Myb. This difference in NO sensitivity may influence the regulation of wild type versus AMV v-Myb protein function.
Brendeford
E M
EM
Department of Biochemistry, University of Oslo, Norway.
Andersson
K B
KB
Gabrielsen
O S
OS
D000818:Animals; D009189:Avian Myeloblastosis Virus / Q000235:genetics; D002645:Chickens; D004247:DNA / Q000378:metabolism*; D004268:DNA-Binding Proteins / Q000235:genetics / Q000378:metabolism*; D004229:Dithiothreitol / Q000737:chemistry; D005978:Glutathione / Q000031:analogs & derivatives / Q000737:chemistry; D009154:Mutation; D009569:Nitric Oxide / Q000138:chemical synthesis / Q000378:metabolism*; D009599:Nitroprusside / Q000737:chemistry; D009603:Nitroso Compounds / Q000737:chemistry; D011485:Protein Binding; D011518:Proto-Oncogene Proteins / Q000235:genetics / Q000378:metabolism*; D020598:Proto-Oncogene Proteins c-myb; D026422:S-Nitrosoglutathione; D015534:Trans-Activators / Q000235:genetics / Q000378:metabolism*
D004268:DNA-Binding Proteins; D009603:Nitroso Compounds; D011518:Proto-Oncogene Proteins; D020598:Proto-Oncogene Proteins c-myb; D015534:Trans-Activators; D009599:Nitroprusside; D009569:Nitric Oxide; D026422:S-Nitrosoglutathione; D004247:DNA; D005978:Glutathione; D004229:Dithiothreitol