title: Continuous flow immunosensor for atrazine detection.
abstract: The hapten atrazine was detected under continuous flow conditions using a micro-column which contained immobilized monoclonal antibodies (Ab) against atrazine and atrazine labeled with alkaline phosphatase (An*). The equilibrium of the antibody-hapten system, was achieved by a continuous flow of the tracer An* through the micro-column containing the immobilized antibodies. The activity of the tracer was monitored continuously, after the micro-column, by an amperometric detector using p-hydroquinone phosphate as substrate. When pulses of unlabeled atrazine (An) were added to the An* flowing continuously through the micro-column, An* bound to the antibody was displaced, with a consequent change of the detector signal. By this method atrazine concentrations in the range 9-180 micrograms/l were monitored under conditions of continuous operation. Since the equilibrium condition for the system Ab-An* was continuously restored by the flow of An* through the micro-column the regeneration of the antibody was not required.
authors.affiliation: Department of Biological Chemistry, University of Padova, Italy.
authors.forename: F
authors.initials: F
authors.lastname: Vianello
authors.forename: L
authors.initials: L
authors.lastname: Signor
authors.forename: A
authors.initials: A
authors.lastname: Pizzariello
authors.forename: M L
authors.initials: ML
authors.lastname: Di Paolo
authors.forename: M
authors.initials: M
authors.lastname: Scarpa
authors.forename: B
authors.initials: B
authors.lastname: Hock
authors.forename: T
authors.initials: T
authors.lastname: Giersch
authors.forename: A
authors.initials: A
authors.lastname: Rigo
mesh_terms: D000818:Animals; D001280:Atrazine / Q000032:analysis*; D015374:Biosensing Techniques; D002417:Cattle; D007118:Immunoassay / Q000379:methods*; D051379:Mice; D012680:Sensitivity and Specificity
chemical_list: D001280:Atrazine