Separation of paraproteins from human plasma by membrane chromatography.
Membrane chromatography using a commercially available blotting membrane was performed in a dead-end filtration mode to separate paraproteins from plasma of patients suffering from paraproteinemia. The affinity membrane was found to display distinct specificity to monoclonal IgG1. A dissociation constant (Kd) of 3.2 microM and a maximum binding capacity of 1.43 mg/cm2 IgG1 paraprotein were obtained from the adsorption isotherm of the affinity membrane. The membrane was found to absorb immunoglobulins species-dependently because no binding of immunoglobulins from mouse, rat and rabbit could be observed.
Birkenmeier
G
G
Institute of Biochemistry, University of Leipzig, Germany.
Dietze
H
H
D000327:Adsorption; D000818:Animals; D000911:Antibodies, Monoclonal / Q000097:blood; D002846:Chromatography, Affinity; D006801:Humans; D007074:Immunoglobulin G / Q000097:blood; D007700:Kinetics; D008567:Membranes, Artificial; D051379:Mice; D010265:Paraproteinemias / Q000097:blood*; D010266:Paraproteins / Q000302:isolation & purification*; D011817:Rabbits; D051381:Rats; D012680:Sensitivity and Specificity; D013045:Species Specificity
D000911:Antibodies, Monoclonal; D007074:Immunoglobulin G; D008567:Membranes, Artificial; D010266:Paraproteins