Catalase prevents estradiol-induced chondrocyte cytotoxicity.
Osteoarthritis is a common geriatric disease and estrogen may play an important role in this disease. Estradiol may cause chondrocyte damage as suggested by in vitro and in vivo data. One of the possible mechanisms of estradiol-induced chondrocyte damage was thought to be related to free radicals. Whether catalase, a known free radical scavenger, can prevent estradiol-induced chondrocyte damage was tested using a chondrocyte culture system. The results of this study suggest that catalase can significantly reduce the estradiol-induced damage to chondrocytes. Apparently, catalase alters the molecular structure of estradiol as indicated by the absorption spectrum of estradiol with time. The modified estradiol may decrease its toxicity to the chondrocytes. However, the contents of free radicals in the treated chondrocytes have no significant difference from the untreated control cells. Studies to further investigate the mechanism or prevention of estradiol-induced chondrocyte damage in osteoarthritis are warranted.
Tsai
C L
CL
Department of Orthopaedic Surgery, College of Medicine, National Taiwan University, Taipei, ROC.
Chang
L Y
LY
Chow
K C
KC
Chan
W K
WK
Chen
Y L
YL
Lu
F J
FJ
D000818:Animals; D002374:Catalase / Q000378:metabolism / Q000494:pharmacology*; D016923:Cell Death / Q000187:drug effects; D002478:Cells, Cultured; D019902:Chondrocytes / Q000166:cytology / Q000187:drug effects*; D004958:Estradiol / Q000633:toxicity*; D016166:Free Radical Scavengers / Q000494:pharmacology; D011817:Rabbits
D016166:Free Radical Scavengers; D004958:Estradiol; D002374:Catalase