[The detection and isolation of fetal nucleated erythrocytes from maternal blood using flow cytometry].
OBJECTIVE
To isolate fetal nucleated red blood cells (NRBCs) from maternal blood and to confirm its fetal origin.


METHODS
Monoclonal antibody against the glycophorin A (GPA) was used to identify nucleated erythrocytes in the peripheral blood of pregnant women. Candidates fetal cells from 3 pregnancies at 16 to 24 weeks of gestation were isolated successfully. Sorted cells were also confirmed using Y-special probe (PY3.4) repeated sequences (SRY) in maternal blood from women carrying male fetuses were detected by polymerase chain reaction (PCR).


RESULTS
The PY3.4 position hybridization rates were 31.5%, 0 and 38% respectively in sorted GPA positive cells (GPA+)of 3 samples by FISH, and the results of GPA+ cells of the 3 cases by PCR were positive, negative and positive respectively, which were manifested by the gender after aborticide.


CONCLUSIONS
This study demonstrates that fetal NRBCs are present in the peripheral blood of pregnant women and it is possible to isolate and detect them.
Li
Z
Z
Department of Clinic Laboratory, Second Teaching Hospital, Lanzhow Medical College, Lanzhow 730030 P. R. China.
Xia
T
T
D004912:Erythrocytes / Q000166:cytology*; D005260:Female; D005312:Fetal Blood / Q000166:cytology*; D005434:Flow Cytometry; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D008297:Male; D011247:Pregnancy