Parathyroid hormone effect on cell-to-cell communication in stromal and osteoblastic cells.
We characterized the formation and regulation of the gap junction in calvarial osteoblasts and in a series of subtypes from marrow stromal cells. The stromal cells included osteogenic, chondro-osteogenic, and endothelial cells. The cell coupling was measured by using fluorescence dye injected into single cells, and its migration to neighboring cells was measured. The functional coupling of cells was highly expressed by the osteoblastic cells. This process is mediated through fast changes in intracellular Ca+2 levels. Calcium ionophore (A 23,187) demonstrated an uncoupling effect on the cells. In addition, the exposure of the cells to the parathyroid hormone increased the formation of the gap junction complex; the highest level was demonstrated in the osteoblastic cells.
Massas
R
R
Department of Physiology and Pharmacology, Sackler Faculty of Medicine, Tel-Aviv University, Israel.
Benayahu
D
D
D001854:Bone Marrow Cells / Q000166:cytology / Q000187:drug effects* / Q000502:physiology; D000001:Calcimycin / Q000494:pharmacology; D002450:Cell Communication / Q000187:drug effects*; D002478:Cells, Cultured; D017629:Gap Junctions / Q000187:drug effects / Q000502:physiology; D007476:Ionophores / Q000494:pharmacology; D010006:Osteoblasts / Q000187:drug effects* / Q000502:physiology; D012886:Skull / Q000166:cytology; D017154:Stromal Cells / Q000187:drug effects / Q000502:physiology; D019379:Teriparatide / Q000494:pharmacology*
D007476:Ionophores; D019379:Teriparatide; D000001:Calcimycin