Accumulation kinetics of viral gene products in cauliflower mosaic virus-infected turnip protoplasts.
The expression of cauliflower mosaic virus (CaMV) genes was studied in a turnip protoplast system. Six CaMV-encoded gene products were detected in infected turnip protoplasts by means of Western blotting. The infected turnip protoplasts showed different patterns of protein accumulation; e.g. an open reading frame (ORF) I-encoded movement protein, an ORF V-encoded reverse transcriptase and an ORF VI-encoded posttranscriptional transactivator representing the early accumulated proteins, an ORF II-encoded aphid transmission factor and an ORF IV-encoded coat protein the late accumulated proteins and an ORF III-encoded DNA binding protein the intermediate protein. The results suggest that the expression of CaMV genes is differentially regulated.
Kobayashi
K
K
Laboratory of Plant Pathology, Faculty of Agriculture, Kyoto University, Japan. kappei@basic.kpu-m.ac.jp
Nakayashiki
H
H
Tsuge
S
S
Mise
K
K
Furusawa
I
I
D015153:Blotting, Western; D001937:Brassica / Q000821:virology*; D017796:Caulimovirus / Q000235:genetics*; D015870:Gene Expression; D005814:Genes, Viral; D007700:Kinetics; D008970:Molecular Weight; D016366:Open Reading Frames; D011523:Protoplasts / Q000821:virology; D014764:Viral Proteins / Q000096:biosynthesis* / Q000235:genetics
D014764:Viral Proteins