title: Molecular cloning and functional expression of a rabbit renal organic cation transporter.
abstract: A cDNA encoding an organic cation transporter (rbOCT1) was isolated from rabbit kidney. The cDNA encodes a 554 amino acid protein that is highly homologous to other mammalian organic cation transporters. rbOCT1 mediated 3H-1-methyl-4-phenylpyridinium (3H-MPP+) transport in Xenopus laevis oocytes was saturable, sensitive to membrane potential, and inhibited by various organic cations. rbOCT1 mRNA transcripts are expressed in the kidney, liver, and intestine.
authors.affiliation: Department of Biopharmaceutical Sciences, University of California, San Francisco 94143, USA.
authors.forename: S
authors.initials: S
authors.lastname: Terashita
authors.forename: M J
authors.initials: MJ
authors.lastname: Dresser
authors.forename: L
authors.initials: L
authors.lastname: Zhang
authors.forename: A T
authors.initials: AT
authors.lastname: Gray
authors.forename: S C
authors.initials: SC
authors.lastname: Yost
authors.forename: K M
authors.initials: KM
authors.lastname: Giacomini
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D015152:Blotting, Northern; D002352:Carrier Proteins / Q000235:genetics*; D003001:Cloning, Molecular; D018076:DNA, Complementary; D007668:Kidney / Q000378:metabolism*; D008297:Male; D008565:Membrane Proteins / Q000235:genetics*; D008969:Molecular Sequence Data; D027702:Organic Cation Transporter 1; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000235:genetics; D011817:Rabbits; D014982:Xenopus laevis
chemical_list: D002352:Carrier Proteins; D018076:DNA, Complementary; D008565:Membrane Proteins; D027702:Organic Cation Transporter 1; D012333:RNA, Messenger