Correlation between basic fibroblast growth factor immunostaining of stromal cells and stromelysin-3 mRNA expression in human breast carcinoma.
We examined the localization of basic fibroblast growth factor (bFGF) in a series of human breast carcinomas using immunohistochemistry. Staining was observed in tumour cells in 15 out of 54 (28%) tumours and in the adjacent stroma in 34 out of 54 (63%) tumours examined. No correlation was observed between positive staining of these two compartments. The relationship between bFGF staining and expression of the metalloprotease stromelysin-3, and between bFGF and microvessel density, was examined. A statistically significant correlation (P < 0.003) was observed between bFGF staining of the stromal compartment and high expression of stromelysin-3 (ST-3; MMP-11) metalloprotease mRNA by stromal cells. In contrast, no correlation was observed between bFGF and intratumour microvessel density (IMD). These results raise the possibility that bFGF may be involved in the induction of stromelysin-3 mRNA expression in breast cancer stroma.
Linder
C
C
Department of Oncology-Pathology, Karolinska Institute and Hospital, Stockholm, Sweden.
Byström
P
P
Engel
G
G
Auer
G
G
Aspenblad
U
U
Strander
H
H
Linder
S
S
D001943:Breast Neoplasms / Q000098:blood supply / Q000201:enzymology* / Q000401:mortality / Q000473:pathology*; D016228:Endothelial Growth Factors / Q000032:analysis; D004790:Enzyme Induction; D005260:Female; D016222:Fibroblast Growth Factor 2 / Q000032:analysis*; D005500:Follow-Up Studies; D006801:Humans; D008222:Lymphokines / Q000032:analysis; D053507:Matrix Metalloproteinase 11; D008666:Metalloendopeptidases / Q000096:biosynthesis*; D008833:Microcirculation / Q000473:pathology; D012333:RNA, Messenger / Q000096:biosynthesis; D012189:Retrospective Studies; D017154:Stromal Cells / Q000201:enzymology / Q000473:pathology; D016019:Survival Analysis; D013997:Time Factors; D014158:Transcription, Genetic; D042461:Vascular Endothelial Growth Factor A; D042442:Vascular Endothelial Growth Factors
D016228:Endothelial Growth Factors; D008222:Lymphokines; D012333:RNA, Messenger; D042461:Vascular Endothelial Growth Factor A; D042442:Vascular Endothelial Growth Factors; D016222:Fibroblast Growth Factor 2; D053507:Matrix Metalloproteinase 11; D008666:Metalloendopeptidases