Gene expression of the phosducin-like protein in the retina.
Phosducin, which is expressed abundantly in the retina, mediates phototransduction or signal transduction cascades by interacting with transducin or G-protein beta gamma-subunits. We have reported on the possibility that phosducin is expressed from a multiple gene family and that each phosducin may mediate G-protein-coupled signal transduction cascades. To elucidate new genes that may mediate signal transduction cascades in the retina and that may form a gene family with phosducin, we screened a bovine retinal cDNA library. During the screening, we identified a cDNA that looked the same as the phosducin-like protein of the rat, as reported by other investigators. We examined the gene expression in rat retina using semiquantitative polymerase chain reaction and compared it to that of other organs. One form generated by alternative splicing expressed in the retina was the same as in other tissues, but, interestingly, another form had differing tissue distribution and was expressed abundantly in the retina.
Abe
T
T
Department of Ophthalmology, Tohoku University School of Medicine, Miyagi, Japan.
Tomita
H
H
Tamai
M
M
D017398:Alternative Splicing; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002352:Carrier Proteins / Q000235:genetics* / Q000378:metabolism; D002417:Cattle; D004247:DNA / Q000032:analysis; D005136:Eye Proteins / Q000235:genetics / Q000378:metabolism; D020555:GTP-Binding Protein Regulators; D019204:GTP-Binding Proteins / Q000037:antagonists & inhibitors; D015870:Gene Expression; D018832:Molecular Chaperones; D008969:Molecular Sequence Data; D009419:Nerve Tissue Proteins / Q000235:genetics* / Q000378:metabolism; D010750:Phosphoproteins / Q000235:genetics / Q000378:metabolism; D010766:Phosphorylation; D016133:Polymerase Chain Reaction; D051381:Rats; D017207:Rats, Sprague-Dawley; D012160:Retina / Q000378:metabolism*; D014018:Tissue Distribution
D002352:Carrier Proteins; D005136:Eye Proteins; D020555:GTP-Binding Protein Regulators; D018832:Molecular Chaperones; D009419:Nerve Tissue Proteins; C498320:Pdcl protein, rat; D010750:Phosphoproteins; C065631:phosducin; D004247:DNA; D019204:GTP-Binding Proteins