Inductions of immediate early genes (IEGS) and ref-1 by human chorionic gonadotropin in murine Leydig cell line (MA-10).
The effect of human chorionic gonadotropin (hCG) on the expression of immediate early genes (IEGs) including all members of fos and jun family, and c-myc was studied using mouse Leydig cell line (MA-10 cells) by Northern blot analyses. In addition, the induction of ref-1 which enhances DNA binding of fos/jun proteins was also analyzed. HCG induced a rapid and transient expression of c-fos, fosB, c-jun, junB, junD and c-myc with a peak at 30 min to 1 h. In contrast, induction of fra-1 mRNA was delayed with a peak at 3 hr. However, fra-2 mRNA was immediately increased by hCG with a peak at 1 h. The ref-1 mRNA was expressed before the stimulation and its level was not altered by hCG at least for 8 hr. The differential induction of IEGs and continuous expression of ref-1 mRNA suggest an important role of their gene products on the regulation of Leydig cell function by hCG.
Suzuki
S
S
Department of Endocrinology and Metabolism, Research Institute of Environmental Medicine, Nagoya University, Japan.
Nagaya
T
T
Suganuma
N
N
Tomoda
Y
Y
Seo
H
H
D000818:Animals; D019757:Carbon-Oxygen Lyases; D006063:Chorionic Gonadotropin / Q000494:pharmacology*; D043603:DNA-(Apurinic or Apyrimidinic Site) Lyase; D004268:DNA-Binding Proteins / Q000235:genetics; D051921:Fos-Related Antigen-2; D005786:Gene Expression Regulation / Q000502:physiology*; D017781:Genes, Immediate-Early / Q000235:genetics*; D006801:Humans; D007984:Leydig Cell Tumor; D007985:Leydig Cells / Q000502:physiology*; D008297:Male; D051379:Mice; D009687:Nuclear Proteins / Q000235:genetics*; D016760:Proto-Oncogene Proteins c-fos / Q000235:genetics; D011519:Proto-Oncogenes / Q000235:genetics; D012333:RNA, Messenger / Q000032:analysis; D051381:Rats; D013736:Testicular Neoplasms; D014157:Transcription Factors / Q000235:genetics; D014407:Tumor Cells, Cultured; D000099169:Fos-Related Antigen 1
D019757:Carbon-Oxygen Lyases; D006063:Chorionic Gonadotropin; D043603:DNA-(Apurinic or Apyrimidinic Site) Lyase; D004268:DNA-Binding Proteins; D051921:Fos-Related Antigen-2; D009687:Nuclear Proteins; D016760:Proto-Oncogene Proteins c-fos; D012333:RNA, Messenger; D014157:Transcription Factors; D000099169:Fos-Related Antigen 1; C497872:FOSL2 protein, human; C497873:Fosl2 protein, mouse; C497874:Fosl2 protein, rat; C085108:APEX1 protein, human; C486216:Apex1 protein, mouse; C470319:Apex1 protein, rat