Identification of R-gene homologous DNA fragments genetically linked to disease resistance loci in Arabidopsis thaliana.
Disease resistance in plants is a desirable economic trait. A number of disease resistance genes from various plant species have been cloned so far. The gene products of some of these can be distinguished by the presence of an N-terminal nucleotide binding site and a C-terminal stretch of leucine-rich repeats. Although these gene products are structurally related, the DNA sequences are poorly conserved. Only parts of the nucleotide binding site share enough DNA identity to design primers for polymerase chain reaction amplification of related DNA sequences. Such primers were used to amplify different resistance-gene-like (RGL) DNA fragments from Arabidopsis thaliana accessions Landsberg erecta and Columbia. Almost all cloned DNA fragments were genetically closely linked with known disease resistance loci. Most RGL fragments were found in a clustered or dispersed multi-copy sequence organization, supporting the supposed correlation of RGL sequences and disease resistance loci.
Aarts
M G
MG
Department of Molecular Biology, DLO-Centre for Plant Breeding and Reproduction Research, Wageningen, The Netherlands.
te Lintel Hekkert
B
B
Holub
E B
EB
Beynon
J L
JL
Stiekema
W J
WJ
Pereira
A
A
D000595:Amino Acid Sequence; D017360:Arabidopsis / Q000235:genetics*; D001483:Base Sequence; D001665:Binding Sites / Q000235:genetics; D002874:Chromosome Mapping; D003001:Cloning, Molecular; D017124:Conserved Sequence; D017931:DNA Primers / Q000235:genetics; D018744:DNA, Plant / Q000235:genetics*; D015870:Gene Expression; D017343:Genes, Plant; D008040:Genetic Linkage; D008969:Molecular Sequence Data; D005810:Multigene Family; D010935:Plant Diseases / Q000235:genetics*; D010940:Plant Proteins / Q000235:genetics; D016133:Polymerase Chain Reaction; D012091:Repetitive Sequences, Nucleic Acid; D017386:Sequence Homology, Amino Acid; D012689:Sequence Homology, Nucleic Acid
D017931:DNA Primers; D018744:DNA, Plant; D010940:Plant Proteins