title: Formation of hydroxyfuranone and hydroxypyranone derivatives with DNA-breaking activity in the Maillard reaction of glucose and albumin under physiological conditions.
abstract: Formation of DNA breaking hydroxyfuranone and hydroxypyranone derivatives in the Maillard reaction of glucose and bovine serum albumin (BSA) under physiological conditions was investigated. A mixture of glucose and BSA was incubated at 37 degrees C in water or in 1 M phosphate buffer (pH 7.4). The ethyl acetate/2-propanol extract of the reaction mixtures showed significant DNA breaking activity against supercoiled DNA especially in the presence of Fe(III) ion. Gas chromatography/mass spectrometry analysis of the mixture revealed the formation of DNA breaking hydroxyfuranones (HMF and DMHF) and hydroxypyranone (DDMP).
authors.affiliation: School of Pharmacy, Tokyo University of Pharmacy and Life Science, Hachioji, Japan.
authors.forename: K
authors.initials: K
authors.lastname: Hiramoto
authors.forename: A
authors.initials: A
authors.lastname: Ishihara
authors.forename: N
authors.initials: N
authors.lastname: Sakui
authors.forename: S
authors.initials: S
authors.lastname: Daishima
authors.forename: K
authors.initials: K
authors.lastname: Kikugawa
mesh_terms: D004249:DNA Damage; D005663:Furans / Q000138:chemical synthesis* / Q000737:chemistry; D008401:Gas Chromatography-Mass Spectrometry; D005947:Glucose / Q000737:chemistry*; D015416:Maillard Reaction; D011714:Pyrans / Q000138:chemical synthesis* / Q000737:chemistry; D012710:Serum Albumin, Bovine / Q000737:chemistry*
chemical_list: D005663:Furans; D011714:Pyrans; D012710:Serum Albumin, Bovine; D005947:Glucose