The somatostatin analogue, octreotide, modifies both steroidogenesis and IGFBP-1 secretion in human luteinizing granulosa cells.
The effect of the somatostatin analogue octreotide on the secretion of progesterone and insulin-like growth factor binding protein-1 (IGFBP-1) from human granulosa-luteal cells was investigated. Octreotide (10(-11), 10(-10) and 10(-9) M) alone induced a significant decrease in progesterone secretion (maximum suppression 69 +/- 5% of control: P < 0.0001). In contrast, treatment with octreotide in combination with human chorionic gonadotrophin (HCG), at 1 IU/ ml potentiated the stimulatory effect of HCG on progesterone secretion (HCG alone 201 +/- 3% of control: HCG + octreotide 10(-9) M 318 +/- 16%: P < 0.001). Treatment with octreotide increased the secretion of IGFBP-1 (maximum stimulation 254 +/- 25% of control: P < 0.01). No effect of HCG was seen on secretion of IGFBP-1. These findings raise the possibility that somatostatin may have a modulatory role in regulating steroidogenesis by the human corpus luteum. Further studies are required to establish the physiological significance of any such function.
Mimuro
T
T
Department of Reproductive Endocrinology, University of Sydney, Westmead Hospital, NSW, Australia.
Smith
H
H
Iwashita
M
M
Illingworth
P J
PJ
D000704:Analysis of Variance; D002478:Cells, Cultured; D004353:Drug Evaluation, Preclinical; D005260:Female; D006107:Granulosa Cells / Q000187:drug effects* / Q000378:metabolism; D006728:Hormones / Q000494:pharmacology*; D006801:Humans; D018970:Insulin-Like Growth Factor Binding Protein 1 / Q000378:metabolism*; D008183:Luteal Phase; D015282:Octreotide / Q000494:pharmacology*; D010053:Ovary / Q000187:drug effects; D011374:Progesterone / Q000378:metabolism; D013256:Steroids / Q000096:biosynthesis*
D006728:Hormones; D018970:Insulin-Like Growth Factor Binding Protein 1; D013256:Steroids; D011374:Progesterone; D015282:Octreotide