title: Improved electroporation of Rhodococcus equi.
abstract: The condition of an electroporation method was re-evaluated for the introduction of foreign plasmid DNA into Rhodococcus equi. The method is based on an electroporation of the bacteria made competent by culturing in a broth containing glycine and by heat shock at 50 degrees C. Transformation of R. equi could be achieved with a chloramphenicol-resistant shuttle vector originating from Rhodococcus fascians at an efficiency of about 10(4) transformants/microgram DNA. The bacteria were also shown to become competent when they were incubated with a chemical transformation buffer prior to washing with an electroporation buffer.
authors.affiliation: National Institute of Animal Health, Tsukuba, Ibaraki, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Sekizaki
authors.forename: T
authors.initials: T
authors.lastname: Tanoue
authors.forename: M
authors.initials: M
authors.lastname: Osaki
authors.forename: Y
authors.initials: Y
authors.lastname: Shimoji
authors.forename: S
authors.initials: S
authors.lastname: Tsubaki
authors.forename: S
authors.initials: S
authors.lastname: Takai
mesh_terms: D003470:Culture Media; D018274:Electroporation / Q000379:methods; D018014:Gene Transfer Techniques; D005990:Glycerol / Q000494:pharmacology; D005998:Glycine / Q000494:pharmacology; D010957:Plasmids; D011136:Polysorbates / Q000494:pharmacology; D016929:Rhodococcus equi / Q000187:drug effects / Q000254:growth & development
chemical_list: D003470:Culture Media; D011136:Polysorbates; D005990:Glycerol; D005998:Glycine