Varying effects of ethanol on transfected cell lines.
The use of transfected cell lines has provided a powerful approach to study the functions of transfected proteins. This approach has also proven useful to determine the effects of acute and chronic ethanol exposure on particular proteins. We show here, however, that the effects of ethanol on transfected proteins vary between transiently transfected cells and stably transfected cell lines. Moreover, we found that the effect of ethanol on a particular protein depends on the plasmid vectors used for the transfection.
Asai
K
K
Ernest Gallo Clinic and Research Center, University of California, San Francisco 94110, USA.
Ishii
A
A
Yao
L
L
Diamond
I
I
Gordon
A S
AS
D000818:Animals; D002461:Cell Line, Transformed; D015500:Chloramphenicol O-Acetyltransferase / Q000235:genetics; D018076:DNA, Complementary / Q000235:genetics; D000431:Ethanol / Q000633:toxicity*; D015870:Gene Expression / Q000187:drug effects; D005910:Glioma; D006801:Humans; D009447:Neuroblastoma; D010957:Plasmids / Q000235:genetics; D017448:Receptors, Dopamine D2 / Q000187:drug effects / Q000235:genetics*; D018047:Receptors, Purinergic P1 / Q000187:drug effects / Q000235:genetics*; D014162:Transfection / Q000187:drug effects* / Q000235:genetics; D014407:Tumor Cells, Cultured / Q000187:drug effects*
D018076:DNA, Complementary; D017448:Receptors, Dopamine D2; D018047:Receptors, Purinergic P1; D000431:Ethanol; D015500:Chloramphenicol O-Acetyltransferase