Mouse/human T-cell hybrids rosetting with sheep erythrocytes.
Hybrid cells have been recovered from selective culture medium after fusion of concanavalin-A-activated human lymphocytes with an AKR mouse thymoma (BW 5147). After 6 months of culture twenty-seven out of forty-nine clones still contained human chromosomes. Human chromosome 6 was present in 89% of these clones, and human X in 70%. Clones from one hybrid line contained several human chromosomes. In twelve of the clones carrying human chromosomes, the rosetting with sheep erythrocytes (SRBC) was 3 times as high as in the BW 5147 cell line. All these clones carried the human chromosome 6, and eight clones contained the human X chromosome as well. In some of these clones (25%) chromosome 6 was the only human one present. In the two clones in which human chromosome 6 was completely missing, the rosetting with SRBC was at the level of the BW line. We therefore suggest that genes on human chromosome 6 are responsible for rosetting with SRBC.
Suomalainen
H A
HA
Folkhälsan Institute of Genetics, Helsinki, Finland.
Goldsby
R A
RA
Osborne
B A
BA
Schröder
J
J
D000818:Animals; D002871:Chromosome Banding; D002874:Chromosome Mapping; D002896:Chromosomes, Human, Pair 6; D006801:Humans; D006822:Hybrid Cells; D051379:Mice; D012397:Rosette Formation; D012756:Sheep; D013601:T-Lymphocytes / Q000276:immunology*