TNF, IL-1 and IL-6 in circulating blood after total-body and localized irradiation in rats.
The levels of TNF, IL-1 and IL-6 in circulating blood of female WAG/Rij rats were assessed both after total-body irradiation (TBI) and localized irradiation of the right hind leg. The results show that enhanced levels of IL-1 in the circulation reflect a stress situation presumably resulting from handling and halothane anesthesia of the animal. Neither localized irradiation nor TBI resulted in further enhanced levels of IL-1. Both TBI and localized irradiation, lead to a small but significant increase in IL-6 levels in serum from circulating blood. After TBI this increase dissipated rapidly, 24 h after TBI increased levels are not found. After localized irradiation IL-6 levels remain elevated for a longer period. Still two weeks after irradiation, the longest time investigated, increased levels were observed. We did not observe increased TNF levels after localized irradiation or after TBI.
Haveman
J
J
Academic Medical Center, University of Amsterdam, Department of Radiotherapy, P.O. Box 22700, Amsterdam, 1100 DE, The Netherlands.
Geerdink
A G
AG
Rodermond
H M
HM
D000818:Animals; D002460:Cell Line; D005260:Female; D006614:Hindlimb / Q000378:metabolism / Q000528:radiation effects*; D007375:Interleukin-1 / Q000097:blood*; D015850:Interleukin-6 / Q000097:blood*; D051381:Rats / Q000097:blood*; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism*; D014916:Whole-Body Irradiation; D014965:X-Rays
D007375:Interleukin-1; D015850:Interleukin-6; D014409:Tumor Necrosis Factor-alpha