Sequence-independent method for in vitro generation of nested deletions for sequencing large DNA fragments.
Fradkov
A F
AF
Shemyakin and Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Science, Miklukho-Maklaya 16/10, V-437, Moscow, 117871, Russia.
Lukyanov
K A
KA
Matz
M V
MV
Diatchenko
L B
LB
Siebert
P D
PD
Lukyanov
S A
SA
D017123:Bacteriophage T7 / Q000235:genetics; D001483:Base Sequence; D003001:Cloning, Molecular / Q000379:methods; D004247:DNA / Q000235:genetics* / Q000302:isolation & purification; D017931:DNA Primers / Q000235:genetics; D003850:Deoxyribonuclease I; D016133:Polymerase Chain Reaction / Q000379:methods; D011401:Promoter Regions, Genetic; D017422:Sequence Analysis, DNA / Q000379:methods*; D017384:Sequence Deletion
D017931:DNA Primers; D004247:DNA; D003850:Deoxyribonuclease I