Interaction of PCB congeners and 2,3,7,8-TCDD in the rat liver: an electron microscope study.
Polyhalogenated aromatic compounds such as polychlorinated biphenyls (PCBs) and polychlorinated dibenzo-p-dioxins continue to be environmental contaminants because of their bioaccumulation in the food chain and resistance to biodegradation. This study was undertaken to determine if WHO-IPCS PCB congeners or 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) individually or their coadministration in rats produced morphological alterations in the liver. Groups (N = 5) of female Sprague Dawley rats received TCDD (0, 2.5, 25, 250, 1,000 ng/kg bw/day) or PCB (0, 2, 20 micrograms/kg bw/day) either alone, or each dose of PCB coadministered with that of TCDD. The test substances were dissolved in corn oil and given by gavage at 0.2 ml/100 g bw/day for 28 days. At the end of the experiment the rats were killed and liver samples were prepared for transmission electron microscopy. Electron micrographs of the liver from animals of the control groups revealed characteristic normal hepatocyte architecture. An increase in smooth endoplasmic reticulum (SER) profiles and a corresponding decrease in the profiles of rough endoplasmic reticulum (RER) proportional to the increased doses of the compounds was revealed in the micrographs. Coadministration of PCBs and TCDD induced greater SER proliferation and a greater decrease in the number of RER profiles compared to either compound administered individually. The PCBs and TCDD at the doses used apparently interacted to induce hepatic ultrastructural alterations. These changes may represent an attempt by the organism to metabolize and neutralize the effects of xenobiotics.
Connell
B J
BJ
Atlantic Veterinary College, University of Prince Edward Island, Charlottetown, Canada.
Singh
A
A
Chu
I
I
D000818:Animals; D004305:Dose-Response Relationship, Drug; D004347:Drug Interactions; D018870:Endoplasmic Reticulum, Rough / Q000187:drug effects / Q000648:ultrastructure; D018871:Endoplasmic Reticulum, Smooth / Q000187:drug effects / Q000648:ultrastructure; D004785:Environmental Pollutants / Q000008:administration & dosage / Q000378:metabolism / Q000633:toxicity; D005260:Female; D008099:Liver / Q000187:drug effects* / Q000378:metabolism / Q000648:ultrastructure*; D008854:Microscopy, Electron; D011078:Polychlorinated Biphenyls / Q000008:administration & dosage* / Q000378:metabolism / Q000633:toxicity*; D000072317:Polychlorinated Dibenzodioxins / Q000008:administration & dosage* / Q000378:metabolism / Q000633:toxicity*; D051381:Rats; D017207:Rats, Sprague-Dawley; D018336:Receptors, Aryl Hydrocarbon / Q000378:metabolism
D004785:Environmental Pollutants; D000072317:Polychlorinated Dibenzodioxins; D018336:Receptors, Aryl Hydrocarbon; D011078:Polychlorinated Biphenyls