title: Effects of preoperative fractionated irradiation on left colonic anastomoses in the rat.
abstract: OBJECTIVE
  Radiotherapy is frequently used as a (neo)adjuvant to surgery in colorectal cancer patients, and because such therapy could influence the integrity of the anastomosis, we decided to investigate the effect of preoperative irradiation on colonic anastomosis.
  
  
  METHODS
  Seventy-two male Wistar rats, weighing 200 to 348 g, were divided into three groups: a control group (I) underwent left colon resection and primary anastomosis (n = 20); a sham irradiated group (II, n = 20); a study group (III) that received fractionated irradiation to the whole pelvis (anterior-posterior pelvic field), for a total dose of 22 Gy, 5.5 Gy per fraction, on four consecutive days with linear accelerator (n = 32). Four days after irradiation, both Groups II and III underwent the same operation as performed in Group I. Within each group, one-half of the animals were anesthetized on the third postoperative day and one-half on the seventh postoperative day. Abdominal wound-healing, anastomotic complications, and anastomotic bursting pressure measurements were recorded. Following these measurements, the anastomotic segment was resected for hydroxyproline content and myeloperoxidase activity.
  
  
  RESULTS
  Irradiated animals had more pronounced weight loss during therapy. There were no differences with abdominal wound-healing, intraperitoneal adhesions, and anastomotic complications between groups. At days 3 and 7, mean bursting pressures of the anastomosis were determined at 36.5 and 208 mmHg in Group I, 34.5 and 228 mmHg in Group II, and 25 and 150 mmHg in Group III, respectively (P < 0.01 Group III vs. both Groups I and II on days 3 and 7). The burst occurred at the anastomosis in all animals tested on the third postoperative day and one in Group I (10 percent), none in Group II, and six in Group III (37.5 percent) on the seventh postoperative day. In addition, hydroxyproline content and myeloperoxidase activity was significantly lower in Group III.
  
  
  CONCLUSIONS
  Although preoperative fractionated irradiation significantly decreased the anastomotic bursting pressure and more burst occurred in the anastomotic line on postoperative day 7, the clinical outcome was similar among the groups.
authors.affiliation: Department of Surgery, Ankara Numune, Turkey.
authors.forename: M A
authors.initials: MA
authors.lastname: Kuzu
authors.forename: C
authors.initials: C
authors.lastname: Köksoy
authors.forename: F H
authors.initials: FH
authors.lastname: Akyol
authors.forename: D
authors.initials: D
authors.lastname: Uzal
authors.forename: T
authors.initials: T
authors.lastname: Kale
authors.forename: E
authors.initials: E
authors.lastname: Demirpence
mesh_terms: D000714:Anastomosis, Surgical; D000818:Animals; D003106:Colon / Q000737:chemistry / Q000503:physiopathology / Q000528:radiation effects* / Q000601:surgery*; D003251:Constriction, Pathologic; D019583:Dose Fractionation, Radiation; D006909:Hydroxyproline / Q000032:analysis; D007415:Intestinal Obstruction / Q000209:etiology; D008297:Male; D011183:Postoperative Complications; D011829:Radiation Dosage; D051381:Rats; D017208:Rats, Wistar; D013529:Surgical Wound Dehiscence; D013530:Surgical Wound Infection; D013718:Tensile Strength; D014945:Wound Healing
chemical_list: D006909:Hydroxyproline