[Interactions between biospecific polymers and MCF7 cells: modulation of cellular proliferation and expression of estrogen receptors].
Numerous studies on interactions between insoluble polymers and cell membrane receptors indicated modulation of cellular proliferation and cell phenotype by these polymers considered as biospecific. We synthesized several biospecific polymers in order to investigate the interactions between polymers and intracellular receptors as estrogen receptors considered as tumoral indicator of breast cancer. Biospecific polymers were obtained by random substitutions of crosslinked polystyrene beads with suitable chemical groups (sulfonate and amino acid sulfamides). These polymers were used as microcarriers for culture of MCF7 cells, a cellular model of human breast cancer. Quantification of MCF7 cell estrogen receptors was determined by radioligand binding assay for different days of cellular proliferation. The data obtained with MCF7 cells cultured on biospecific polymers show an inverse relationship between polymer induced inhibition of cell proliferation and polymer induced increase of estrogen receptors. Similar inverse relationship was obtained with MCF7 cell cultured on standard polystyrene tissue culture plates. The various interaction between insoluble polymers and MCF7 cells could be related to the proportion and the nature of the substitutive chemical groups. These biospecific polymers could presents sites of interaction with cell membrane receptors leading to modulation of cell biological activity. The different insoluble polymers were used as preliminary models: a practical application could be a methodology of cellular selection using soluble biospecific polymers (for example chemically modified dextrans).
Mestries
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Laboratoire de recherche sur la croissance cellulaire, CNRS URA 1813, université Paris-XII Val-de-Marne, Faculté des Sciences-IUT de Créteil.
Kern
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Avramoglou
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D001667:Binding, Competitive; D001943:Breast Neoplasms / Q000378:metabolism*; D002455:Cell Division / Q000187:drug effects*; D004958:Estradiol / Q000494:pharmacology; D005260:Female; D006801:Humans; D011137:Polystyrenes / Q000737:chemistry / Q000494:pharmacology*; D011960:Receptors, Estrogen / Q000187:drug effects*; D013329:Structure-Activity Relationship; D013997:Time Factors; D014407:Tumor Cells, Cultured
D011137:Polystyrenes; D011960:Receptors, Estrogen; D004958:Estradiol
Interactions entre polymères biospécifiques et lignée cellulaire MCF7: modulation de la prolifération cellulaire et de l'expression des récepteurs des oestrogènes.