Interaction of phospholipase C gamma 1 via its COOH-terminal SRC homology 2 domain with synaptojanin.
The role of the phospholipase C gamma 1 (PLC gamma 1) in signal transduction was investigated by characterizing its interactions with proteins that may represent components of a novel signalling pathway. A 145-kDa protein that binds SH2 domain of PLC gamma 1 was purified from rat brain. The sequence of peptide derived from the purified binding protein now identify it as synaptojanin, a nerve terminal protein that has been implicated in the endocytosis of fused synaptic vesicles and shown to be a member of the inositol polyphosphate 5-phosphatase family. We demonstrate here stable association of PLC gamma 1 with synaptojanin, a protein that not only binds carboxyl terminal SH2 domain of PLC gamma 1, but also inhibits PLC gamma 1 activity.
Ahn
S J
SJ
Department of Molecular Biology, Kon-Kuk University, Chungju, Korea.
Han
S J
SJ
Mo
H J
HJ
Chung
J K
JK
Hong
S H
SH
Park
T K
TK
Kim
C G
CG
D000595:Amino Acid Sequence; D000818:Animals; D001921:Brain / Q000201:enzymology; D004789:Enzyme Activation / Q000187:drug effects / Q000235:genetics; D004815:Epidermal Growth Factor / Q000378:metabolism; D007527:Isoenzymes / Q000037:antagonists & inhibitors / Q000502:physiology*; D008970:Molecular Weight; D009419:Nerve Tissue Proteins / Q000502:physiology*; D051966:Phospholipase C gamma; D010744:Phosphoric Monoester Hydrolases / Q000502:physiology*; D011485:Protein Binding / Q000235:genetics; D051381:Rats; D020794:Receptor Protein-Tyrosine Kinases / Q000378:metabolism; D017479:Receptors, Platelet-Derived Growth Factor / Q000378:metabolism; D011993:Recombinant Fusion Proteins / Q000378:metabolism; D013379:Substrate Specificity; D010738:Type C Phospholipases / Q000037:antagonists & inhibitors / Q000502:physiology*; D018909:src Homology Domains / Q000235:genetics / Q000502:physiology*
D007527:Isoenzymes; D009419:Nerve Tissue Proteins; D011993:Recombinant Fusion Proteins; D004815:Epidermal Growth Factor; D020794:Receptor Protein-Tyrosine Kinases; D017479:Receptors, Platelet-Derived Growth Factor; C097702:synaptojanin; D010744:Phosphoric Monoester Hydrolases; D010738:Type C Phospholipases; D051966:Phospholipase C gamma