Localisation of arginine vasopressin V1a receptors on sympatho-adrenal preganglionic neurones.
Vasopressin-containing nerve terminals are present in the spinal cord of several species. This study was designed to determine whether sympatho-adrenal preganglionic neurones (SPN) express vasopressin receptors (VPRs). SPN in the spinal cord were revealed by retrograde labelling of Fluorogold following its unilateral injection into the adrenal medulla of 12-20 day postnatal rats. VPRs were simultaneously visualised in the Fluorogold-labelled slices of spinal cord using a recently developed biotinylated vasopressin receptor antagonist [1-phenylacetyl,2-O-methyl-D-tyrosine,6-arginine,8-arginine,9-lysinam ide(Nepsilon-biotinamidocaproamide)]vasopressin, PhAcAL(Btn)VP. The VPR:PhAcAL(Btn)VP complexes were visualised either with Texas Red-conjugated avidin or with a Vectastain avidin:alkaline phosphatase detection kit. These dual-labelling experiments revealed VPRs to be present in the spinal grey matter and to be particularly dense in the intermediate grey matter and adjacent regions of the ventral horn. Many SPN were associated with receptor-specific labelling of PhAcAL(Btn)VP, thereby demonstrating that VPRs are expressed by these neurones. These VPRs were pharmacologically defined as the V1a subtype. It is concluded that sympatho-adrenal preganglionic neurones express VPRs and that these are of the V1a subtype. The distribution of VPRs is not, however, restricted to these SPN in the spinal cord.
Sermasi
E
E
Department of Physiology, The Medical School, The University of Birmingham, UK.
Howl
J
J
Wheatley
M
M
Coote
J H
JH
D000311:Adrenal Glands / Q000166:cytology / Q000378:metabolism* / Q000648:ultrastructure; D000818:Animals; D000831:Animals, Newborn; D065092:Antidiuretic Hormone Receptor Antagonists; D001339:Autonomic Fibers, Preganglionic / Q000187:drug effects / Q000378:metabolism* / Q000648:ultrastructure; D005456:Fluorescent Dyes; D051381:Rats; D017207:Rats, Sprague-Dawley; D017483:Receptors, Vasopressin / Q000378:metabolism*; D013116:Spinal Cord / Q000166:cytology / Q000378:metabolism / Q000648:ultrastructure; D013266:Stilbamidines; D013564:Sympathetic Nervous System / Q000166:cytology / Q000378:metabolism* / Q000648:ultrastructure
C049774:2-hydroxy-4,4'-diamidinostilbene, methanesulfonate salt; D065092:Antidiuretic Hormone Receptor Antagonists; D005456:Fluorescent Dyes; D017483:Receptors, Vasopressin; D013266:Stilbamidines