Microbiota associated with experimental peri-implantitis and periodontitis in adult Macaca mulatta monkeys.
This study examines the microbiota associated with the progression of experimental peri-implantitis and periodontitis induced concurrently in partially edentulous adult monkeys. Root-form and plate-form implants with fixed prosthesis in place for at least 12 months and their corresponding opposite molar teeth were ligated for 6 months. The microbiota in plaque around these ligated dental implants and molars were studied at 0, 1, 2, 3, and 6 months post-ligation. Plaque samples were analyzed by dark-field microscopy and selective and non-selective culture. Putative periodontal pathogens were detected as a major component of the microbiota cultured from plaque samples obtained from experimental peri-implantitis sites. Overall, the types and relative proportions of putative periodontal pathogens in plaque associated with ligature-induced peri-implantitis and ligature-induced periodontitis were similar. Only levels of anaerobic Actinomyces and spirochetes were significantly different between both sites. Spirochete levels were significantly higher at peri-implantitis sites when compared with levels at periodontitis sites after 6 months, and spirochete levels increased significantly between 0 and 6 months post-ligation at implant sites. Levels of spirochetes correlated significantly with probing depth and bone loss at peri-implantitis sites. Overall, Actinomyces levels were higher at periodontitis sites. Porphyromonas species were not detected continuously as part of the peri-implantitis microbiota. In conclusion, this study finds that the microbiota associated with the progression of experimental peri-implantitis and periodontitis occurring concurrently in partially edentulous mouths are similar.
Eke
P I
PI
Emory University, Yerkes Regional Primate Research Center, Department of Molecular Medicine, Atlanta, GA 30322, USA. ekep@msm.edu
Braswell
L D
LD
Fritz
M E
ME
D000190:Actinomyces / Q000254:growth & development / Q000302:isolation & purification; D016976:Aggregatibacter actinomycetemcomitans / Q000254:growth & development / Q000302:isolation & purification; D016301:Alveolar Bone Loss / Q000209:etiology / Q000382:microbiology; D000818:Animals; D001419:Bacteria / Q000145:classification* / Q000254:growth & development / Q000302:isolation & purification; D015169:Colony Count, Microbial; D003758:Dental Implantation, Endosseous / Q000009:adverse effects*; D015921:Dental Implants / Q000009:adverse effects*; D003773:Dental Plaque / Q000382:microbiology; D017267:Dental Prosthesis Design; D019094:Dental Prosthesis, Implant-Supported; D018450:Disease Progression; D005500:Follow-Up Studies; D007576:Jaw, Edentulous, Partially / Q000534:rehabilitation / Q000601:surgery; D008253:Macaca mulatta; D010514:Periodontal Pocket / Q000209:etiology / Q000382:microbiology; D010518:Periodontitis / Q000209:etiology / Q000382:microbiology*; D017861:Porphyromonas / Q000254:growth & development / Q000302:isolation & purification; D018713:Prevotella intermedia / Q000254:growth & development / Q000302:isolation & purification; D013144:Spirochaetales / Q000254:growth & development / Q000302:isolation & purification
D015921:Dental Implants