Induction of flat morphology in K-ras-transformed fibroblasts by lycorine, an alkaloid isolated from the tropical plant Eucharis grandiflora.
In the course of screening for Ras function inhibitors from plant extracts, we isolated lycorine from a chloroform extract of Eucharis grandiflora leaves. Lycorine induced flat morphology in K-ras-NRK cells after treatment for 2-3 days, whereas its morphological effect on NRK cells was weaker. Lycorine was found to inhibit protein synthesis specifically in cultured K-ras-NRK cells. It also lowered the cellular amount of Ras in 2-3 days.
Kushida
N
N
Department of Applied Chemistry, Faculty of Science and Technology, Keio University, Yokohama, Japan.
Atsumi
S
S
Koyano
T
T
Umezawa
K
K
D000470:Alkaloids / Q000302:isolation & purification / Q000494:pharmacology*; D047151:Amaryllidaceae Alkaloids; D000818:Animals; D002455:Cell Division / Q000187:drug effects; D002471:Cell Transformation, Neoplastic / Q000187:drug effects*; D005347:Fibroblasts / Q000166:cytology / Q000187:drug effects*; D011905:Genes, ras / Q000187:drug effects* / Q000502:physiology*; D010617:Phenanthridines / Q000302:isolation & purification / Q000494:pharmacology*; D010936:Plant Extracts / Q000302:isolation & purification / Q000494:pharmacology*; D018515:Plant Leaves / Q000737:chemistry; D010946:Plants, Medicinal / Q000737:chemistry; D051381:Rats
D000470:Alkaloids; D047151:Amaryllidaceae Alkaloids; D010617:Phenanthridines; D010936:Plant Extracts; C015330:lycorine