Expression of biglycan, decorin and proteoglycan-100/CSF-1 in normal and fibrotic human liver.
The immunohistochemical expression of the three small chondroitin/dermatan sulphate proteoglycans biglycan, decorin and proteoglycan-100 (PG-100), the proteoglycan form of colony-stimulating factor-1 (CSF-1), was studied in normal and fibrotic human liver. In normal liver tissue biglycan and decorin were clearly seen in the space of Disse, which was in contrast to a faint staining of PG-100. Biglycan and decorin were additionally detected around small bile ducts and in vessel walls. In patients with HBs-Ag positive, chronic active hepatitis decorin as well as biglycan showed strong immunoreactivity in fibrotic areas. In contrast to normal liver, PG-100 was visualized in bile duct epithelia. Therefore, PG-100 could serve as an immunohistochemical marker of the involvement of the bile duct system in chronic active hepatitis and progressive liver fibrosis.
Högemann
B
B
Department of Medicine B, University of Münster, Federal Republic of Germany.
Edel
G
G
Schwarz
K
K
Krech
R
R
Kresse
H
H
D000328:Adult; D000368:Aged; D058578:Biglycan; D001652:Bile Ducts / Q000737:chemistry / Q000378:metabolism / Q000473:pathology; D001808:Blood Vessels / Q000737:chemistry / Q000378:metabolism / Q000473:pathology; D058575:Decorin; D016326:Extracellular Matrix Proteins; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D008099:Liver / Q000737:chemistry / Q000378:metabolism* / Q000473:pathology; D008103:Liver Cirrhosis / Q000378:metabolism; D016173:Macrophage Colony-Stimulating Factor / Q000032:analysis / Q000096:biosynthesis*; D008297:Male; D008875:Middle Aged; D011509:Proteoglycans / Q000032:analysis / Q000096:biosynthesis*; D014018:Tissue Distribution
C546884:BGN protein, human; D058578:Biglycan; C546878:DCN protein, human; D058575:Decorin; D016326:Extracellular Matrix Proteins; D011509:Proteoglycans; D016173:Macrophage Colony-Stimulating Factor