title: Ovariectomy augments B lymphopoiesis and generation of monocyte-macrophage precursors in rat bone marrow.
abstract: To investigate the effects of estrogen depletion on hematopoiesis and bone turnover, female rats were either ovariectomized (OVX) or sham operated and killed at 1, 2, 3, and 4 wk postsurgery. Flow cytometric analysis of bone marrow cells (BMC) revealed that, in close temporal association with the rise in bone turnover as measured by bone histomorphometry, the number of Thy 1.1+ and KiB1R+ BMC increased two- to threefold in OVX rats relative to sham controls. The Thy 1.1+ BMC were further characterized as Thy 1.1+/KiB1R+ and Thy 1.1+/HIS24+ double-positive cells of the B cell lineage. A transient rise in ED1+ myeloid cells expressing a lysosomal antigen specific for the monocyte-macrophage and osteoclast lineage coincided with the upregulation of osteoclast numbers in OVX rats at 2 wk postsurgery, but the number of ED8+ myelomonocytic BMC remained unchanged. Administration of estradiol prevented the rise in Thy 1.1+, KiB1R+, and ED1+ BMC in OVX animals. Our study indicates that ovariectomy upregulates B lymphopoiesis in rat bone marrow and increases myeloid cell differentiation into the monocyte-macrophage and possibly also the osteoclast lineage.
authors.affiliation: Institute of Physiology, Physiological Chemistry and Animal Nutrition, Ludwig Maximilians University, Munich, Germany.
authors.forename: R G
authors.initials: RG
authors.lastname: Erben
authors.forename: S
authors.initials: S
authors.lastname: Raith
authors.forename: J
authors.initials: J
authors.lastname: Eberle
authors.forename: M
authors.initials: M
authors.lastname: Stangassinger
mesh_terms: D000818:Animals; D001402:B-Lymphocytes / Q000166:cytology*; D001835:Body Weight / Q000187:drug effects; D001854:Bone Marrow Cells / Q000166:cytology*; D002469:Cell Separation; D004967:Estrogens / Q000494:pharmacology / Q000502:physiology; D005260:Female; D005434:Flow Cytometry; D006410:Hematopoiesis; D006412:Hematopoietic Stem Cells / Q000502:physiology*; D008264:Macrophages / Q000166:cytology*; D009000:Monocytes / Q000166:cytology*; D009929:Organ Size / Q000187:drug effects; D010052:Ovariectomy; D010053:Ovary / Q000502:physiology*; D051381:Rats; D014599:Uterus / Q000187:drug effects / Q000502:physiology
chemical_list: D004967:Estrogens