title: Genetic variation of Porphyromonas gingivalis genes encoding gingipains, cysteine proteinases with arginine or lysine specificity.
abstract: Porphyromonas gingivalis hemagglutinating cysteine proteinases (gingipains) are considered as important virulence factors in the development of adult periodontitis. Using Southern blot analysis it was determined that genes of three distinct but related gingipains (gingipain-R1, gingipain-R2 and gingipain-K) were present in all tested strains including HG66, ATCC 33277, W50, E-20-1, EM-3, 381, A7436, and IKG5, with a region encoding a part of the hemagglutinin domain of gingipain-R1 and gingipain-K showing considerable variability. In contrast, the loci encoding gingipain-R1 (rgp1) and gingipain-R2 (rgp2) were strongly conserved excluding the concurrent occurrence of other gingipain-R-like genes such as cpgR and agp. Significantly, no evidence could be found to support the expression of a gene coding for the putative proteinase porphypain, an enzyme suggested to have both gingipain-R and gingipain-K activity.
authors.affiliation: Institute of Molecular Biology, Jagiellonian University, Kraków, Poland.
authors.forename: J
authors.initials: J
authors.lastname: Mikolajczyk-Pawlinska
authors.forename: T
authors.initials: T
authors.lastname: Kordula
authors.forename: N
authors.initials: N
authors.lastname: Pavloff
authors.forename: P A
authors.initials: PA
authors.lastname: Pemberton
authors.forename: W C
authors.initials: WC
authors.lastname: Chen
authors.forename: J
authors.initials: J
authors.lastname: Travis
authors.forename: J
authors.initials: J
authors.lastname: Potempa
mesh_terms: D018829:Adhesins, Bacterial; D000595:Amino Acid Sequence; D001120:Arginine / Q000235:genetics*; D001483:Base Sequence; D001665:Binding Sites; D003001:Cloning, Molecular; D003546:Cysteine Endopeptidases / Q000235:genetics*; D004269:DNA, Bacterial; D005798:Genes, Bacterial; D014644:Genetic Variation; D000080867:Gingipain Cysteine Endopeptidases; D006388:Hemagglutinins / Q000235:genetics*; D006801:Humans; D008239:Lysine / Q000235:genetics*; D008969:Molecular Sequence Data; D016966:Porphyromonas gingivalis / Q000201:enzymology* / Q000235:genetics; D017422:Sequence Analysis, DNA
chemical_list: D018829:Adhesins, Bacterial; D004269:DNA, Bacterial; D000080867:Gingipain Cysteine Endopeptidases; D006388:Hemagglutinins; D001120:Arginine; D003546:Cysteine Endopeptidases; D008239:Lysine