Cellular expression of the cytolytic factor in earthworms Eisenia foetida.
Coelomic fluid of earthworms contains a 42 kDa protein designated CCF-1 (coelomic cytolytic factor 1), which accounts for approximately 40% of cytolytic activity of the entire coelomic fluid. CCF-1 was documented to be present on cells of the mesenchymal lining of the coelomic cavity as well as on free coelomocytes. Both cellular and humoral levels of CCF-1 were significantly increased after parenteral injection of endotoxin. Moreover, CCF-1 seems to be involved in cell mediated cytotoxicity, because cytotoxic activity is blocked in the presence of anti-CCF-1 monoclonal antibody (mAb).
Bilej
M
M
Department of Immunology, Institute of Microbiology, Academy of Sciences of the Czech Republic, Vídenská, Prague.
Rossmann
P
P
Sinkora
M
M
Hanusová
R
R
Beschin
A
A
Raes
G
G
De Baetselier
P
P
D000818:Animals; D003601:Cytotoxicity Tests, Immunologic; D003603:Cytotoxins / Q000096:biosynthesis*; D037102:Lectins; D008070:Lipopolysaccharides / Q000494:pharmacology; D009835:Oligochaeta / Q000276:immunology*
D003603:Cytotoxins; D037102:Lectins; D008070:Lipopolysaccharides; C094795:coelomic cytolytic factor 1, Eisenia fetida