Enhanced detection of human telomerase activity.
The detectability of telomerase activity in human cells almost always correlates with indefinite proliferation capability (immortalization). To make quantitative statements about telomerase activity levels, complete extraction of the telomerase activity is needed. A series of detergents was tested for this purpose, and a combination of NP-40 and sodium deoxycholate (NaDOC) was found to be the most efficient for extracting telomerase activity. Tumor-derived cell lines originally thought to contain differing amounts of telomerase on the basis of the original CHAPS bases extraction procedures have nearly equivalent amounts of activity when extracted with the NP-40/NaDOC lysis buffer. These results indicate that these lysis conditions can be used to extract telomerase activity more efficiently from tumor-derived cell lines.
Norton
J C
JC
Department of Cell Biology and Neuroscience, The University of Texas Southwestern Medical Center at Dallas, 75235-9039, USA.
Holt
S E
SE
Wright
W E
WE
Shay
J W
JW
D001671:Biochemistry / Q000379:methods*; D002021:Buffers; D003902:Detergents; D006801:Humans; D009369:Neoplasms / Q000201:enzymology*; D019098:Telomerase / Q000032:analysis* / Q000378:metabolism*; D014407:Tumor Cells, Cultured
D002021:Buffers; D003902:Detergents; D019098:Telomerase