title: Oxygen free radical scavenger properties of dehydroepiandrosterone.
abstract: The microsomes from dehydroepiandrosterone (DHEA)-supplemented animals are good hydroxyl radical scavengers, as demonstrated through electron spin resonance and deoxyribose degradation. The ability of DHEA-supplemented microsomes to react with superoxide radical was also demonstrated through the inhibition of nitroblue-tetrazolium reduction determined by superoxide radicals produced in a hypoxanthine-xanthine oxidase system. DHEA-enriched microsomes, obtained from acutely DHEA-treated rats, become resistant to iron-dependent lipid peroxidation triggered by H2O2/FeSO4 and ascorbate/FeSO4. The direct addition of DHEA to microsomes from untreated rats failed to prevent iron-dependent lipid peroxidation, even if the microsomes were preincubated with DHEA for up to 15 min, indicating that in vivo transformation is required before antioxidant action can be exerted.
authors.forename: E
authors.initials: E
authors.lastname: Tamagno
authors.forename: M
authors.initials: M
authors.lastname: Aragno
authors.forename: G
authors.initials: G
authors.lastname: Boccuzzi
authors.forename: M
authors.initials: M
authors.lastname: Gallo
authors.forename: S
authors.initials: S
authors.lastname: Parola
authors.forename: B
authors.initials: B
authors.lastname: Fubini
authors.forename: G
authors.initials: G
authors.lastname: Poli
authors.forename: O
authors.initials: O
authors.lastname: Danni
mesh_terms: D000818:Animals; D003687:Dehydroepiandrosterone / Q000378:metabolism* / Q000494:pharmacology; D016166:Free Radical Scavengers / Q000378:metabolism*; D007501:Iron / Q000494:pharmacology; D008862:Microsomes, Liver / Q000187:drug effects / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar
chemical_list: D016166:Free Radical Scavengers; D003687:Dehydroepiandrosterone; D007501:Iron