Rapid activation of JNK1 in UV-B irradiated epidermal keratinocytes.
Jun N-terminal kinase (JNK1) is a member of a family of stress-activated protein kinases which are activated by many forms of stress including UV radiation, resulting in the phosphorylation of c-Jun, ATF-2, Elk-1 and p53. As UV-B radiation is mainly responsible for ultraviolet (UV)-induced skin cancers, we chose to elucidate JNK1 activation in keratinocytes which represent a UV-relevant cell system. We have demonstrated rapid activation of JNK1 in a keratinocyte cell line, C50, in response to multiple doses of UV-B irradiation. JNK1 activation occurred within 1 min, peaked by 10 min and returned to near basal levels within 2 h following the UV-B treatments. Our data provide the first evidence to show that keratinocytes do respond to multiple doses of the physiologically relevant UV-B radiation through rapid activation of the JNK1 pathway.
Ramaswamy
N T
NT
Department of Pathology, University of Kansas Medical Center, Kansas City 66160-7410, USA.
Ronai
Z
Z
Pelling
J C
JC
D017871:Calcium-Calmodulin-Dependent Protein Kinases / Q000378:metabolism*; D002461:Cell Line, Transformed; D004307:Dose-Response Relationship, Radiation; D004789:Enzyme Activation; D000078404:Epidermal Cells; D004817:Epidermis / Q000201:enzymology / Q000528:radiation effects*; D048031:JNK Mitogen-Activated Protein Kinases; D015603:Keratinocytes / Q000201:enzymology / Q000528:radiation effects*; D020928:Mitogen-Activated Protein Kinases; D014466:Ultraviolet Rays
D017871:Calcium-Calmodulin-Dependent Protein Kinases; D048031:JNK Mitogen-Activated Protein Kinases; D020928:Mitogen-Activated Protein Kinases