title: Laser pyrolysis products: sampling procedures, cytotoxic and genotoxic effects.
abstract: The use of lasers in medical applications has grown enormously in the last few years. Recent chemical analysis of the laser pyrolysis products revealed that aerosols generated by pyrolytic decomposition of tissue could be health hazards. Therefore we analysed the genotoxic and mutagenic effects of laser pyrolysis products from different types of porcine tissue. The tissues were irradiated with a surgical CO2 laser and the generated aerosols were sampled as particulate fractions as well as low and highly volatile fractions. Then human leukocytes were incubated with the pyrolysis products and subjected to the comet assay. The results of the comet assay indicated the pyrolysis products being inducers of DNA damage. The ability to induce genotoxic effects turned out to be strongly dependent on the type of tissue that had been irradiated during laser treatment. To check whether the pyrolysis products also have mutagenic properties the Salmonella mutagenicity assay was performed. The particulate aerosol fractions of skin, muscle tissue and liver tissue clearly proved to be mutagenic in TA98 in the presence of S9 mix. There was no mutagenic effect detectable without metabolic activation. In conclusion, our experiments showed that the laser pyrolysis products originating from porcine tissues induced very potent genotoxic as well as mutagenic effects and therefore they could be potential health hazards for humans.
authors.affiliation: Department of Clinical Physiology, Occupational and Social Medicine, University of Ulm, Germany.
authors.forename: B
authors.initials: B
authors.lastname: Stocker
authors.forename: T
authors.initials: T
authors.lastname: Meier
authors.forename: T M
authors.initials: TM
authors.lastname: Fliedner
authors.forename: U
authors.initials: U
authors.lastname: Plappert
mesh_terms: D000273:Adipose Tissue / Q000737:chemistry / Q000528:radiation effects; D000328:Adult; D000336:Aerosols / Q000302:isolation & purification / Q000633:toxicity*; D000818:Animals; D002470:Cell Survival; D004249:DNA Damage; D005260:Female; D006801:Humans; D007834:Lasers / Q000009:adverse effects*; D007962:Leukocytes / Q000187:drug effects; D008099:Liver / Q000737:chemistry / Q000528:radiation effects; D018482:Muscle, Skeletal / Q000737:chemistry / Q000528:radiation effects; D009152:Mutagenicity Tests; D009153:Mutagens / Q000633:toxicity*; D012486:Salmonella typhimurium / Q000187:drug effects / Q000235:genetics; D012867:Skin / Q000737:chemistry / Q000528:radiation effects; D013552:Swine
chemical_list: D000336:Aerosols; D009153:Mutagens