Preparative chromatography of furosemide 1-O-acyl-glucuronide from urine using micronized amberiite XAD-2 and its application to other 1-O-acyl-glucuronides.
Furosemide 1-O-acyl glucuronide (Fgnd) was extracted from the urine following oral administration of furosemide. The crude Fgnd was applied to micronized Amberlite XAD-2 column (2.5 cm i.d. x 90 cm length, 75-500 microns particle size). The purified Fgnd was identified by mass spectrometry and beta-glucuronidase treatment. This method was also applicable to the purification of glucuronide of tolmetin (nonsteroidal anti-inflammatory drug, NSAID), suggesting that it was applicable to the other NSAIDs, most of which were known to be metabolized to acyl-glucuronides.
Mizuma
T
T
Department of Biopharmaceutics, Tokyo University of Pharmacy and Life Science, Japan.
Benet
L Z
LZ
Lin
E T
ET
D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000032:analysis; D001562:Benzimidazoles / Q000032:analysis; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D002852:Chromatography, Ion Exchange / Q000379:methods; D002994:Clofibrate / Q000031:analogs & derivatives / Q000032:analysis; D005439:Flufenamic Acid / Q000031:analogs & derivatives / Q000032:analysis; D005665:Furosemide / Q000031:analogs & derivatives* / Q000652:urine; D005965:Glucuronates / Q000652:urine*; D005966:Glucuronidase / Q000378:metabolism; D007213:Indomethacin / Q000031:analogs & derivatives / Q000032:analysis; D013058:Mass Spectrometry; D015394:Molecular Structure; D011339:Probenecid / Q000031:analogs & derivatives / Q000032:analysis; D014046:Tolmetin / Q000031:analogs & derivatives / Q000032:analysis
D000894:Anti-Inflammatory Agents, Non-Steroidal; D001562:Benzimidazoles; D005965:Glucuronates; D005439:Flufenamic Acid; C036234:furosemide glucuronide; D005665:Furosemide; D014046:Tolmetin; D005966:Glucuronidase; D002994:Clofibrate; D011339:Probenecid; D007213:Indomethacin