Trans-activation of the Tetrahymena ribozyme by its P2-2.1 domains.
The Tetrahymena group I self-splicing intron contains peripheral domains P2-2.1. Mutant introns lacking these domains are hardly active. We found that if an independently prepared P2-2.1 RNA is added in trans, it efficiently enhances the catalytic activity of an intron lacking the domains. P2-2.1 RNA together with the previously identified activator, P5abc RNA, of the Tetrahymena intron can activate the intron lacking both of them. The trans-activation depends on the long-range interaction between P2.1 and P9.1 domains.
Ikawa
Y
Y
Department of Chemistry, Faculty of Science, Kyoto University, Sakyo-ku, Kyoto 606-01, Japan.
Shiraishi
H
H
Inoue
T
T
D000818:Animals; D001483:Base Sequence; D007438:Introns; D008969:Molecular Sequence Data; D009154:Mutation; D009690:Nucleic Acid Conformation; D016337:RNA, Catalytic / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D013768:Tetrahymena / Q000235:genetics*; D015534:Trans-Activators
D016337:RNA, Catalytic; D015534:Trans-Activators