Integrated cell isolation and polymerase chain reaction analysis using silicon microfilter chambers.
White blood cells are isolated from whole blood in silicon-glass 4.5-microliter microchips containing a series of 3.5-micron feature-sized 'weir-type' filters, formed by an etched silicon dam spanning the flow chamber. Genomic DNA targets, e.g., dystrophin gene, can be directly amplified using the polymerase chain reaction (PCR) from the white cells isolated on the filters. This dual function microchip provides a means to simplify nucleic acid analyses by integrating in a single device two key steps in the analytical procedure, namely, cell isolation and PCR.
Wilding
P
P
Department of Pathology and Laboratory Medicine, University of Pennsylvania, Philadelphia 19104, USA.
Kricka
L J
LJ
Cheng
J
J
Hvichia
G
G
Shoffner
M A
MA
Fortina
P
P
D002469:Cell Separation / Q000379:methods; D004247:DNA / Q000032:analysis; D016189:Dystrophin / Q000097:blood / Q000235:genetics; D004912:Erythrocytes / Q000166:cytology / Q000378:metabolism; D005898:Glass; D006454:Hemoglobins / Q000378:metabolism; D006801:Humans; D007962:Leukocytes / Q000737:chemistry / Q000166:cytology*; D008851:Micropore Filters; D016133:Polymerase Chain Reaction / Q000295:instrumentation* / Q000379:methods; D012825:Silicon
D016189:Dystrophin; D006454:Hemoglobins; D004247:DNA; D012825:Silicon