title: [Spread of an intramuscularly administered live gE-negative BHV1 marker vaccine in 2 cattle farms].
abstract: Cattle are vaccinated with a live gE-negative BHV1-marker vaccine as a part of a programme to eradicate bovine herpes virus 1 in the Netherlands. To investigate whether this vaccine is transmitted to unvaccinated cattle in a herd after intramuscular administration, cattle from two herds were vaccinated: of a herd of 114 BHV1-antibody negative cattle on one farm, 45 animals were vaccinated, and of a dairy herd of 55 cattle in another farm, all 10 BHV1-positive animals were vaccinated. Vaccination was repeated after about a month. Antibodies against BHV1 were determined in all unvaccinated cattle to detect potential BHV1-antibody responses. Only one unvaccinated cow (from the dairy herd) showed BHV1 seroconversion. However, this antibody response was detected by a gE-ELISA as well as by a gB-ELISA. This indicates that the animal was infected by a field virus and not by the gE-negative vaccine virus. Thus intramuscularly administered live gE-negative BHV1 marker vaccine did not spread to unvaccinated cattle (n =154) on two farms.
authors.forename: W H
authors.initials: WH
authors.lastname: van der Poel
authors.forename: J J
authors.initials: JJ
authors.lastname: Hage
mesh_terms: D000818:Animals; D000914:Antibodies, Viral / Q000302:isolation & purification; D002417:Cattle / Q000276:immunology; D002418:Cattle Diseases / Q000517:prevention & control*; D004797:Enzyme-Linked Immunosorbent Assay / Q000662:veterinary; D005260:Female; D006566:Herpesviridae Infections / Q000517:prevention & control / Q000635:transmission / Q000662:veterinary*; D014759:Viral Envelope Proteins / Q000276:immunology*; D014764:Viral Proteins; D014765:Viral Vaccines
chemical_list: D000914:Antibodies, Viral; D014759:Viral Envelope Proteins; D014764:Viral Proteins; D014765:Viral Vaccines; C046998:bovine herpesvirus type-1 glycoproteins
vernacular_title: Onderzoek naar spreiding van een intramusculair toegediend levend gE-negatief BHV1 markervaccin op twee rundveebedrijven.