Identification of iron-regulated proteins of Mycobacterium tuberculosis and cloning of tandem genes encoding a low iron-induced protein and a metal transporting ATPase with similarities to two-component metal transport systems.
Iron plays a central role in the pathogenesis of Mycobacterium tuberculosis, the principal causative agent of tuberculosis. To learn more about iron acquisition by this bacterium, its iron regulated proteins (IRPs) were investigated. Seven IRPs were identified - three increased by high iron concentrations, and four by low iron concentrations. The smallest protein induced by low iron, Irp10, is tightly iron regulated as it is virtually absent in bacteria cultured in the presence of high iron concentrations. The gene (irpA ) encoding this protein and an adjacent open reading frame, mtaA, were cloned and sequenced. The protein encoded by mtaA (Mta72) has striking homology to metal transporting P-type ATPases. This study suggests that Irp10 and Mta72 function as a two-component metal transport system in M. tuberculosis.
Calder
K M
KM
Department of Medicine, School of Medicine, University of California, Los Angeles, California, 90095, USA.
Horwitz
M A
MA
D018528:ATP-Binding Cassette Transporters; D000251:Adenosine Triphosphatases; D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000737:chemistry / Q000235:genetics; D001483:Base Sequence; D001692:Biological Transport; D002352:Carrier Proteins; D002462:Cell Membrane / Q000201:enzymology; D003001:Cloning, Molecular; D015964:Gene Expression Regulation, Bacterial / Q000187:drug effects; D005798:Genes, Bacterial / Q000235:genetics*; D007501:Iron / Q000494:pharmacology*; D008565:Membrane Proteins / Q000235:genetics*; D008670:Metals / Q000378:metabolism; D008969:Molecular Sequence Data; D008970:Molecular Weight; D009169:Mycobacterium tuberculosis / Q000235:genetics* / Q000378:metabolism; D016366:Open Reading Frames / Q000235:genetics; D012329:RNA, Bacterial / Q000032:analysis; D012333:RNA, Messenger / Q000032:analysis; D017421:Sequence Analysis; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid
D018528:ATP-Binding Cassette Transporters; D001426:Bacterial Proteins; D002352:Carrier Proteins; C112063:Irp10 protein, Mycobacterium tuberculosis; D008565:Membrane Proteins; D008670:Metals; C112062:Mta72 protein, bacteria; D012329:RNA, Bacterial; D012333:RNA, Messenger; D007501:Iron; D000251:Adenosine Triphosphatases