High-performance liquid chromatographic determination of seratrodast and its metabolites in human serum and urine.
A high-performance liquid chromatographic (HPLC) method was developed for the simultaneous determination of seratrodast, a new antiasthmatic drug, and its metabolites (M-I to M-III) in human serum and urine. The method for serum and urine with and without enzymatic hydrolysis using beta-glucuronidase involved liquid-liquid extraction and chemical oxidation with iron(III) chloride. The compounds in the extract were analyzed using HPLC with UV detection at 266 nm. The detection limits of seratrodast, M-I, M-II and M-III in serum and urine were 5-10 and 5-20 ng/ml, respectively, and those of deconjugated compounds in urine were 10-50 ng/ml. The method was applicable for human serum and urine from clinical trials.
Ohta
R
R
Drug Analysis & Pharmacokinetics Research Laboratories, Takeda Chemical Industries, Ltd., Osaka, Japan.
Amano
T
T
Yamashita
K
K
Motohashi
M
M
D018927:Anti-Asthmatic Agents; D016227:Benzoquinones / Q000097:blood* / Q000378:metabolism / Q000652:urine*; D002712:Chlorides; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D005290:Ferric Compounds; D005966:Glucuronidase; D006538:Heptanoic Acids / Q000097:blood* / Q000378:metabolism / Q000652:urine*; D006801:Humans; D006868:Hydrolysis; D008832:Microchemistry; D010084:Oxidation-Reduction; D011448:Prostaglandin Antagonists; D011786:Quality Control; D012680:Sensitivity and Specificity
D018927:Anti-Asthmatic Agents; D016227:Benzoquinones; D002712:Chlorides; D005290:Ferric Compounds; D006538:Heptanoic Acids; D011448:Prostaglandin Antagonists; C060230:seratrodast; D005966:Glucuronidase; C024555:ferric chloride