Purification and molecular cloning of a chitinase expressed in the hepatopancreas of the penaeid prawn Penaeus japonicus.
A protein with chitinase activity was purified from the hepatopancreas of the penaeid prawn Penaeus japonicus, and the amino acid sequences of several amino acid fragments were determined. A cDNA clone was isolated and sequenced which contained the coding sequence of the enzyme. The conceptually translated protein (named Pjchi-3) was a member of the chitinase family based on sequence similarities to chitinases of non-crustacean species, as was the case with Pjchi-1 and 2, two chitinase homologues previously isolated from P. japonicus.
Watanabe
T
T
Laboratory of Molecular Biology of Marine Organisms, University of Tokyo, Japan. toshi@ori.u-tokyo.ac.jp
Kono
M
M
Aida
K
K
Nagasawa
H
H
D000595:Amino Acid Sequence; D000818:Animals; D060829:Arthropod Proteins; D001181:Arthropods; D001483:Base Sequence; D002688:Chitinases / Q000737:chemistry*; D003001:Cloning, Molecular; D004064:Digestive System / Q000201:enzymology*; D006031:Glycosylation; D008969:Molecular Sequence Data; D008970:Molecular Weight; D033561:Penaeidae; D012333:RNA, Messenger / Q000378:metabolism; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA
D060829:Arthropod Proteins; D012333:RNA, Messenger; D002688:Chitinases; C111673:Pjchi-3 protein, Penaeus japonicus