title: A product of DAN, a novel candidate tumour suppressor gene, is secreted into culture medium and suppresses DNA synthesis.
abstract: Our previous studies have shown that the DAN gene product possesses an ability to revert phenotypes of transformed rat fibroblasts and represents a candidate tumour suppressor gene for neuroblastoma. In the present study, characterisation of DAN was carried out using rat fibroblast 3Y1 cells and their DAN-overexpressor counterparts (S-9). The N-terminal region of DAN (amino acids 1-24) was highly hydrophobic and DAN protein was found to be secreted into the culture medium. When DAN was treated with PNGase F, a enzyme that cleaves most N-linked carbohydrate residues, the mobility of both cytoplasmic and secreted DAN was increased in SDS-polyacrylamide gel electrophoresis, suggesting DAN is N-glycosylated, irrespective of its localisation. When partially purified, DAN was able, when added to the culture, to suppress DNA synthesis of Rous sarcoma virus-transformed 3Y1 cells, which lack the expression of DAN.
authors.affiliation: Division of Biochemistry, Chiba Cancer Centre Research Institute, Japan.
authors.forename: Y
authors.initials: Y
authors.lastname: Nakamura
authors.forename: T
authors.initials: T
authors.lastname: Ozaki
authors.forename: A
authors.initials: A
authors.lastname: Nakagawara
authors.forename: S
authors.initials: S
authors.lastname: Sakiyama
mesh_terms: D000818:Animals; D002461:Cell Line, Transformed; D004261:DNA Replication; D016147:Genes, Tumor Suppressor; D009363:Neoplasm Proteins / Q000737:chemistry / Q000378:metabolism* / Q000502:physiology; D009419:Nerve Tissue Proteins; D009447:Neuroblastoma / Q000235:genetics / Q000378:metabolism*; D010449:Peptide Mapping; D011506:Proteins / Q000235:genetics / Q000378:metabolism*; D051381:Rats
chemical_list: C085763:Nbl1 protein, rat; D009363:Neoplasm Proteins; D009419:Nerve Tissue Proteins; D011506:Proteins