Nucleotide sequences of glycoprotein I and E genes of equine herpesvirus type 4.
The nucleotide sequences of the glycoprotein I (gI) and E (gE) genes of equine herpesvirus type 4 (EHV-4) strain TH20 were determined. The predicted region encoding the EHV-4 gI gene is 1,263 nucleotides, corresponding to a polypeptide of 420 amino acids in length. The predicted region encoding the EHV-4 gE gene is 1,647 nucleotides, corresponding to a polypeptide of 548 amino acids in length. The EHV-4 gI and gE genes show 74% and 85% identity at the amino acid level with those of equine herpesvirus type 1 (EHV-1), respectively. Furthermore, we have found an open reading frame homologous to the EHV-1 gene 75, which overlaps in part with the 3' end of EHV-4 gE gene. These sequence data will be useful for development of a modified live vaccine against equine herpesvirus type 1 and 4 infections.
Damiani
A M
AM
Department of Veterinary Microbiology, Faculty of Agriculture, University of Tokyo, Japan.
Matsumura
T
T
Yokoyama
N
N
Maeda
K
K
Miyazawa
T
T
Kai
C
C
Mikami
T
T
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002478:Cells, Cultured; D016679:Genome, Viral; D006736:Horses; D007668:Kidney; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D015183:Restriction Mapping; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid; D018140:Varicellovirus / Q000235:genetics* / Q000502:physiology; D014759:Viral Envelope Proteins / Q000096:biosynthesis / Q000737:chemistry / Q000235:genetics*
C111144:Equid herpesvirus 1 gl; D014759:Viral Envelope Proteins; C111845:glycoprotein I, Equine herpesvirus 4; C111846:glycoprotein e, Equid herpesvirus 4