title: Role of mononuclear cells of IgA nephropathy on ICAM-1 expression in mesangial cells.
abstract: OBJECTIVE
  To investigate the possible role of mononuclear cells and their products in the pathogenesis of IgA nephropathy, in vitro expression of ICAM-1 on cultured mouse mesangial cell (MC) was examined after stimulation with mononuclear cell culture supernatant from patients with IgA nephropathy.
  
  
  METHODS
  Peripheral blood mononuclear cells (PBMC) were isolated and cultured from 18 patients with primary IgA nephropathy, 8 normal controls and 5 patients with non-IgA nephropathy (FSGS 1, MGN 3, MPGN 1). ICAM-1 expression on cultured mouse MC by TNF-alpha, IL-1 beta and culture supernants of PBMC were analyzed using a cell ELISA method. The concentration of IL-1 beta and TNF-alpha in culture supernatants was measured by using a commercially available radioimmunoassay kit.
  
  
  RESULTS
  Addition of human recombinant TNF-alpha induced an increased ICAM-1 expression in a dose-dependent manner. The expression of ICAM-1 was further increased after co-stimulation with TNF-alpha and IL-1 beta. Addition of PBMC culture supernatants into mouse MC induced significantly higher expression of ICAM-1 by supernatants from the patients with IgA nephropathy compared with that from normal controls. The concentration of TNF-alpha and IL-1 beta in supernatants from the patients with IgA nephropathy was significantly higher than that from those with non-IgA nephropathy.
  
  
  CONCLUSIONS
  TNF-alpha and IL-1 released from mononuclear cells induced the up-regulation of ICAM-1 expression and this may be related to the immune pathogenesis of IgA nephropathy.
authors.affiliation: Department of Internal Medicine/Nephrology, College of Medicine, Kyunghee University, Seoul, Korea.
authors.forename: T W
authors.initials: TW
authors.lastname: Lee
authors.forename: J K
authors.initials: JK
authors.lastname: Park
authors.forename: J H
authors.initials: JH
authors.lastname: Ahn
authors.forename: C G
authors.initials: CG
authors.lastname: Ihm
authors.forename: M J
authors.initials: MJ
authors.lastname: Kim
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D005920:Glomerular Mesangium / Q000166:cytology / Q000276:immunology; D005922:Glomerulonephritis, IGA / Q000209:etiology / Q000276:immunology*; D006801:Humans; D018799:Intercellular Adhesion Molecule-1 / Q000378:metabolism*; D007375:Interleukin-1 / Q000378:metabolism / Q000494:pharmacology; D007963:Leukocytes, Mononuclear / Q000276:immunology; D051379:Mice; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism / Q000494:pharmacology
chemical_list: D007375:Interleukin-1; D014409:Tumor Necrosis Factor-alpha; D018799:Intercellular Adhesion Molecule-1