Redox properties of an H-subunit-depleted photosynthetic reaction center from Rhodopseudomonas viridis.
Recently, we reported that a H-subunit-depleted photosynthetic reaction center (RC-H) was purified from purple nonsulfer photosynthetic bacterium Rhodopseudomonas viridis (Rps. viridis) using a strong detergent sodium alkyl ether sulfate. We compared the redox properties of a native photosynthetic reaction center (RC) and RC-H of Rps. viridis. In RC-H prepared by our method, secondary quinone (QB) was removed while primary quinone (QA) was retained. Absorption spectrum of RC-H was similar to that of RC. After reconstitution of ubiquinone 10 into QB sites, RC-H showed electron transfer activity that was the same as that for native RC. This is the first report about the redox properties of RC-H of Rps. viridis.
Hara
M
M
National Institute of Bioscience and Human Technology, Agency of Industrial Science and Technology (AIST), Ministry of International Trade and Industry (MITI), 1-1 Higashi, Tsukuba, Ibaraki 305-8566, Japan. mhara@ccmail.nibh.go.jp
Kaneko
T
T
Nakamura
C
C
Asada
Y
Y
Miyake
J
J
D004579:Electron Transport; D010084:Oxidation-Reduction; D010782:Photolysis; D045322:Photosynthetic Reaction Center Complex Proteins / Q000737:chemistry / Q000378:metabolism*; D012241:Rhodopseudomonas / Q000378:metabolism*; D013053:Spectrophotometry
D045322:Photosynthetic Reaction Center Complex Proteins