Purification of the alkaloid lycorine and simultaneous analysis of ascorbic acid and lycorine by micellar electrokinetic capillary chromatography.
The pyrrolophenanthridine alkaloid lycorine has frequently been used as a specific inhibitor to help elucidate the function of ascorbic acid (vitamin C) in a wide range of biological processes. It was recently reported that this function can be exercised by inhibiting the activity of L-galactono-1,4-lactone dehydrogenase, the terminal enzyme of ascorbic acid biosynthesis, although working with the purified enzyme, we have been unable to repeat this result. Here, we present a procedure for the purification and analysis of lycorine by high-performance liquid chromatography from two Crinum species and describe for the first time a method that allows the simultaneous analysis of ascorbic acid and lycorine in tissue extracts of Crinum asiaticum by micellar electrokinetic chromatography.
Davey
M W
MW
Department of Genetics, Flanders Institute for Biotechnology (VIB), Universiteit Gent, Belgium.
Persiau
G
G
De Bruyn
A
A
Van Damme
J
J
Bauw
G
G
Van Montagu
M
M
D047151:Amaryllidaceae Alkaloids; D001205:Ascorbic Acid / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D002855:Chromatography, Thin Layer; D004563:Electrochemistry; D019075:Electrophoresis, Capillary / Q000379:methods*; D009682:Magnetic Resonance Spectroscopy; D013058:Mass Spectrometry; D008823:Micelles; D010617:Phenanthridines / Q000032:analysis / Q000302:isolation & purification*; D018515:Plant Leaves / Q000737:chemistry; D018517:Plant Roots / Q000737:chemistry
D047151:Amaryllidaceae Alkaloids; D008823:Micelles; D010617:Phenanthridines; C015330:lycorine; D001205:Ascorbic Acid