An E-box sequence acts as a transcriptional activator for BC1 RNA expression by RNA polymerase III in the brain.
BC1 RNA is a small cytoplasmic RNA that is transcribed by RNA polymerase III (Pol III) in the rodent nervous system. In addition to essential intragenic promoter elements for Pol III, the BC1 RNA gene has five E-box sequences (CANNTG) in its 5' flanking region. Deletion analysis using an in vitro transcription system revealed that the region containing the E2 site (CAATTG) was necessary for effective transcription of BC1 RNA. A construct with point mutations within the E2 site showed reduced transcriptional activity. Furthermore, DNaseT I protection and gel retardation assays demonstrated that the E2 site was recognized specifically by a brain nuclear protein(s). These results suggest that the upstream E-box sequence and its binding protein may be involved in the regulation by Pol III of preferential BC1 RNA expression in the brain.
Kobayashi
S
S
Division of Molecular Cell Biology, College of Pharmacy, Nihon University, Narashinodai, Chiba, Funabashi, 274-8555, Japan.
Anzai
K
K
D000818:Animals; D001921:Brain / Q000201:enzymology*; D004268:DNA-Binding Proteins / Q000235:genetics*; D003850:Deoxyribonuclease I / Q000378:metabolism; D005786:Gene Expression Regulation / Q000235:genetics; D016296:Mutagenesis / Q000235:genetics; D009687:Nuclear Proteins / Q000378:metabolism; D017354:Point Mutation / Q000235:genetics; D011485:Protein Binding; D012320:RNA Polymerase III / Q000737:chemistry*; D062085:RNA, Long Noncoding; D022661:RNA, Untranslated; D051381:Rats; D012261:Ribonucleoproteins / Q000235:genetics*; D020736:Ribonucleoproteins, Small Cytoplasmic; D017422:Sequence Analysis, DNA; D014158:Transcription, Genetic / Q000235:genetics; D015533:Transcriptional Activation / Q000502:physiology*
C489663:Bc1 long-non-coding RNA, rat; D004268:DNA-Binding Proteins; D009687:Nuclear Proteins; D062085:RNA, Long Noncoding; D022661:RNA, Untranslated; D012261:Ribonucleoproteins; D020736:Ribonucleoproteins, Small Cytoplasmic; D012320:RNA Polymerase III; D003850:Deoxyribonuclease I