title: cDNA characterization and chromosome mapping of the human GAS2 gene.
abstract: Murine Gas2 is a microfilament-associated protein whose expression is increased at growth arrest in mammalian cells. During apoptosis, Gas2 is specifically cleaved at its C-terminus by a still unknown ICE-like protease, and the processed protein induces dramatic rearrangements in the cytoskeleton when overexpressed in several cell types. Here we report the characterization of a cDNA encoding the human homologue of Gas2, showing high conservation with the murine counterpart at the protein level. Fluorescence in situ hybridization analysis and radiation hybrid mapping localized the GAS2 gene on human chromosome 11p14.3-p15.2, in a region homologous to the gas2 region on mouse chromosome 7.
authors.affiliation: Laboratorio Nazionale Consorzio Interuniversitario Biotecnologie, Trieste, Italy.
authors.forename: L
authors.initials: L
authors.lastname: Collavin
authors.forename: M
authors.initials: M
authors.lastname: Buzzai
authors.forename: S
authors.initials: S
authors.lastname: Saccone
authors.forename: L
authors.initials: L
authors.lastname: Bernard
authors.forename: C
authors.initials: C
authors.lastname: Federico
authors.forename: G
authors.initials: G
authors.lastname: DellaValle
authors.forename: C
authors.initials: C
authors.lastname: Brancolini
authors.forename: C
authors.initials: C
authors.lastname: Schneider
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D017209:Apoptosis / Q000235:genetics; D002453:Cell Cycle / Q000235:genetics; D002874:Chromosome Mapping / Q000379:methods*; D002880:Chromosomes, Human, Pair 11 / Q000235:genetics; D018076:DNA, Complementary / Q000737:chemistry*; D006801:Humans; D051379:Mice; D008840:Microfilament Proteins / Q000235:genetics*; D008969:Molecular Sequence Data; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid
chemical_list: D018076:DNA, Complementary; C490936:GAS2 protein, human; C099165:Gas2 protein, mouse; D008840:Microfilament Proteins