title: Identification of a BRCA1-associated kinase with potential biological relevance.
abstract: A biochemical approach was used to identify proteins which interact with human BRCA1. Through this work, a kinase activity which co-purifies with BRCA1 has been identified. This kinase activity, which phosphorylates BRCA1 in vitro, was originally identified in Sf9 insect cells but is also present in cells of human origin including breast and ovarian carcinoma cell lines. The BRCA1 kinase activity in vitro is associated with a fragment of BRCA1 encompassing amino acids 329-435. This peptide is also phosphorylated in various human cell lines. A computer-assisted sequence analysis revealed that this peptide was a potential substrate for phosphorylation by PKA, PKC, or CKII. However, phosphorylation by these kinases could not be demonstrated in vitro indicating the presence of another kinase activity. Phosphorylation in vitro requires a minimal domain of BRCA1 encompassing amino acids 379-408. Notably, deletion of this minimal domain abolishes growth suppression by BRCA1 indicating that this domain, as well as phosphorylation within this domain, may be important for BRCA1 function.
authors.affiliation: Cancer Research, Lilly Research Laboratories, Eli Lilly and Company, Indianapolis, Indiana 46285, USA.
authors.forename: T F
authors.initials: TF
authors.lastname: Burke
authors.forename: K S
authors.initials: KS
authors.lastname: Cocke
authors.forename: S J
authors.initials: SJ
authors.lastname: Lemke
authors.forename: E
authors.initials: E
authors.lastname: Angleton
authors.forename: G W
authors.initials: GW
authors.lastname: Becker
authors.forename: R P
authors.initials: RP
authors.lastname: Beckmann
mesh_terms: D000595:Amino Acid Sequence; D019313:BRCA1 Protein / Q000378:metabolism*; D001665:Binding Sites; D001943:Breast Neoplasms / Q000201:enzymology*; D005260:Female; D006801:Humans; D008969:Molecular Sequence Data; D010766:Phosphorylation; D014407:Tumor Cells, Cultured
chemical_list: D019313:BRCA1 Protein