The role of integrin beta 1 in human dental pulp cell adhesion on laminin and fibronectin.
OBJECTIVE
This study is to identify the expression of integrin beta 1 in human dental pulp cells and the role of integrin beta 1 in pulp cell adhesion on extracellular matrix protein laminin and fibronectin.


METHODS
Immunoblot detection of integrin beta 1 in human pulp cells was with the use of monoclonal anti-beta 1 antibody. Dental pulp cell adhesion assay on extracellular matrix protein laminin and fibronectin and blocking cell adhesion was performed with monoclonal anti-beta 1 antibody.


RESULTS
Integrin beta 1 was identified in human dental pulp cells. Pulp cells adhered and spread on both laminin and fibronectin. Monoclonal anti-beta 1 antibody inhibited human dental pulp cells adhesion on laminin but not on fibronectin.


CONCLUSIONS
Integrin beta 1 was expressed on human dental pulp cells and mediated cell adhesion on laminin. Human dental pulp cells also adhered on fibronectin but the adhesion was not regulated by beta 1 integrin.
Zhu
Q
Q
Department of Restorative Dentistry and Endodontology, School of Dental Medicine, University of Connecticut Health Center, Farmington, Conn., USA.
Safavi
K E
KE
Spangberg
L S
LS
D000911:Antibodies, Monoclonal; D002448:Cell Adhesion; D002465:Cell Movement; D048429:Cell Size; D002478:Cells, Cultured; D003782:Dental Pulp / Q000166:cytology* / Q000502:physiology; D004591:Electrophoresis, Polyacrylamide Gel; D005353:Fibronectins / Q000502:physiology*; D005786:Gene Expression Regulation; D006801:Humans; D015151:Immunoblotting; D019012:Integrin beta1 / Q000235:genetics / Q000502:physiology*; D007797:Laminin / Q000502:physiology*; D012967:Sodium Dodecyl Sulfate
D000911:Antibodies, Monoclonal; D005353:Fibronectins; D019012:Integrin beta1; D007797:Laminin; D012967:Sodium Dodecyl Sulfate