staggerer phenotype in retinoid-related orphan receptor alpha-deficient mice.
Retinoid-related orphan receptor alpha (RORalpha) is a member of the nuclear receptor superfamily. To study its physiological role we generated null-mutant mice by targeted insertion of a lacZ reporter gene encoding the enzyme beta-galactosidase. In heterozygous RORalpha+/- mice we found beta-galactosidase activity, indicative of RORalpha protein expression, confined to the central nervous system, skin and testis. In the central nervous system, the RORalpha gene is expressed in cerebellar Purkinje cells, the thalamus, the suprachiasmatic nuclei, and retinal ganglion cells. In skin, RORalpha is strongly expressed in the hair follicle, the epidermis, and the sebaceous gland. Finally, the peritubular cells of the testis and the epithelial cells of the epididymis also strongly express RORalpha. Recently, it was reported that the ataxic mouse mutant staggerer (sg/sg) is caused by a deletion in the RORalpha gene. The analysis of the cerebellar and the behavioral phenotype of homozygous RORalpha-/- mice proves identity to sg/sg mice. Although the absence of RORalpha causes dramatic developmental effects in the cerebellum, it has no apparent morphological effect on thalamus, hypothalamus, and retina. Similarly, testis and skin of RORalpha-/- mice display a normal phenotype. However, the pelage hair of both sg/sg and RORalpha-/- is significantly less dense and when shaved shows reluctance to regrow.
Steinmayr
M
M
Geneva Biomedical Research Institute, Glaxo Wellcome Research and Development S.A., 4, chemin des Aulx, 1228 Plan-les-Ouates, Switzerland.
André
E
E
Conquet
F
F
Rondi-Reig
L
L
Delhaye-Bouchaud
N
N
Auclair
N
N
Daniel
H
H
Crépel
F
F
Mariani
J
J
Sotelo
C
C
Becker-André
M
M
D000818:Animals; D001522:Behavior, Animal / Q000502:physiology; D002531:Cerebellum / Q000502:physiology*; D005786:Gene Expression Regulation; D051379:Mice; D008817:Mice, Mutant Strains; D009154:Mutation; D009419:Nerve Tissue Proteins / Q000172:deficiency / Q000235:genetics*; D057094:Nuclear Receptor Subfamily 1, Group F, Member 1; D009928:Organ Specificity; D018160:Receptors, Cytoplasmic and Nuclear / Q000172:deficiency / Q000235:genetics*; D015534:Trans-Activators / Q000172:deficiency / Q000235:genetics*
D009419:Nerve Tissue Proteins; D057094:Nuclear Receptor Subfamily 1, Group F, Member 1; D018160:Receptors, Cytoplasmic and Nuclear; D015534:Trans-Activators