Development of a polymerase chain reaction assay for Mycoplasma salivarium by using the nucleotide sequence within aminopeptidase My gene.
A polymerase chain reaction assay for a 278-nucleotide DNA fragment within aminopeptidase My gene of Mycoplasma salivarium was developed. The assay amplified M. salivarium DNA, but did not amplify DNAs of other mollicutes, bacteria and mammalian cells. The detection limit of the assay was 10 fg of DNA, approximately equivalent to 10 organisms.
Yoshikawa
T
T
Department of Oral Bacteriology, Hokkaido University School of Dentistry, Sapporo, Japan.
Shibata
K
K
Kaga
M
M
Sato
Y
Y
Oguchi
H
H
Totsuka
Y
Y
Watanabe
T
T
D000328:Adult; D000626:Aminopeptidases / Q000235:genetics*; D000818:Animals; D001426:Bacterial Proteins; D001431:Bacteriological Techniques; D002460:Cell Line; D002648:Child; D002675:Child, Preschool; D017931:DNA Primers; D004269:DNA, Bacterial / Q000032:analysis; D005260:Female; D006801:Humans; D008297:Male; D051379:Mice; D009174:Mycoplasma / Q000201:enzymology / Q000235:genetics* / Q000302:isolation & purification*; D009175:Mycoplasma Infections / Q000175:diagnosis*; D016133:Polymerase Chain Reaction / Q000379:methods*; D012463:Saliva / Q000382:microbiology; D012680:Sensitivity and Specificity; D017422:Sequence Analysis, DNA
D001426:Bacterial Proteins; D017931:DNA Primers; D004269:DNA, Bacterial; D000626:Aminopeptidases; C095710:aminopeptidase My, Mycoplasma salivarium