Determination of glycolic acid in cosmetic products by solid-phase extraction and reversed-phase ion-pair high-performance liquid chromatography.
A procedure has been developed for the assay of glycolic acid in cosmetic products by reversed-phase high-performance liquid chromatography using an ion-pairing method. After dissolution in tetrahydrofuran-water (90:10, v/v), samples were purified by solid-phase extraction using silica-based strong anion-exchange cartridges and analysed directly on an Ultrasphere ODS column with UV detection at 210 nm and methanol-phosphate buffer (2:98, v/v), containing tetrabutylammonium iodide, as the mobile phase. Recovery of glycolic acid from different cosmetic matrices was between 92.4 and 96.2% and the precision of the method was better than 5.4% relative standard deviation. The procedure is rapid, simple, selective and it is suitable for routine analyses of commercial cosmetics.
Scalia
S
S
Dipartimento di Scienze Farmaceutiche, Università di Ferrara, Italy.
Callegari
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R
Villani
S
S
D002021:Buffers; D002851:Chromatography, High Pressure Liquid; D003358:Cosmetics / Q000032:analysis*; D004655:Emulsions; D006016:Glycolates / Q000032:analysis*; D007202:Indicators and Reagents; D012997:Solvents; D013056:Spectrophotometry, Ultraviolet
D002021:Buffers; D003358:Cosmetics; D004655:Emulsions; D006016:Glycolates; D007202:Indicators and Reagents; D012997:Solvents; C031149:glycolic acid