cDNA cloning of mouse prolyl endopeptidase and its involvement in DNA synthesis by Swiss 3T3 cells.
A cDNA for mouse prolyl endopeptidase (PEP) was cloned and its nucleotide sequence determined. The overall amino acid sequence identity between mouse and other mammalian PEPs was about 96%. A specific inhibitor of PEP, N-benzyloxycarbonyl-thioprolyl-thioprolinal- dimethylacetal (ZTTA), inhibited DNA synthesis by Swiss 3T3 cells. Mouse PEP was shown to be localized partly in restricted nuclear regions. These results suggest that PEP participates in mammalian DNA synthesis.
Ishino
T
T
Faculty of Pharmaceutical Sciences, The University of Tokyo, Bunkyo-ku, Tokyo 113, Japan.
Ohtsuki
S
S
Homma
K i
Ki
Natori
S
S
D016475:3T3 Cells / Q000187:drug effects / Q000201:enzymology*; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D015139:Blotting, Southern; D002455:Cell Division / Q000187:drug effects; D002467:Cell Nucleus / Q000201:enzymology; D003001:Cloning, Molecular; D003593:Cytoplasm / Q000201:enzymology; D004247:DNA / Q000096:biosynthesis*; D019084:Fluorescent Antibody Technique, Indirect; D015151:Immunoblotting; D051379:Mice; D008969:Molecular Sequence Data; D000085822:Prolyl Oligopeptidases; D012697:Serine Endopeptidases / Q000235:genetics* / Q000276:immunology / Q000378:metabolism*; D013844:Thiazoles / Q000494:pharmacology
C105467:N-benzyloxycarbonyl-thioprolyl-thioprolinal-dimethylacetal; D013844:Thiazoles; D004247:DNA; D012697:Serine Endopeptidases; D000085822:Prolyl Oligopeptidases