Development of a scintillation proximity assay for peroxisome proliferator-activated receptor gamma ligand binding domain.
A scintillation proximity assay for peroxisome proliferator-activated receptor gamma ligand binding domain is described. Scintillation proximity offers an equilibrium method for detecting ligands that is both cost effective and fully automatable. The method described here is the first reported scintillation proximity assay for a peroxisome proliferator-activated receptor. The design of this system is generic in nature, allowing it to be adapted for other ligand binding proteins.
Nichols
J S
JS
Department of Molecular Biochemistry, Glaxo Wellcome, Inc., Research Triangle Park, North Carolina 27709-3398, USA.
Parks
D J
DJ
Consler
T G
TG
Blanchard
S G
SG
D000595:Amino Acid Sequence; D019921:Biotinylation; D002850:Chromatography, Gel; D006801:Humans; D008024:Ligands; D008969:Molecular Sequence Data; D011869:Radioligand Assay / Q000379:methods; D018160:Receptors, Cytoplasmic and Nuclear / Q000737:chemistry / Q000378:metabolism*; D000077154:Rosiglitazone; D012588:Scintillation Counting; D019809:Streptavidin; D013844:Thiazoles / Q000378:metabolism*; D045162:Thiazolidinediones; D014157:Transcription Factors / Q000737:chemistry / Q000378:metabolism*
D008024:Ligands; D018160:Receptors, Cytoplasmic and Nuclear; D013844:Thiazoles; D045162:Thiazolidinediones; D014157:Transcription Factors; D000077154:Rosiglitazone; D019809:Streptavidin