Antibody to a Helicobacter pylori species specific antigen in patients with adenocarcinoma of the stomach.
This study attempted to identify a possible antibody response to Helicobacter pylori, which is associated with patients with adeno-carcinoma of the stomach. By using proteins of H. pylori as the antigen, pooled sera from gastric cancer and non-cancer patients were used as the first antibody for Western blot analysis. Antibody responses to a 26 kD secreted protein were observed in pooled cancer sera, but not in pooled sera from non-cancer patients. The protein was purified, while amino acid sequences revealed that it was a H. pylori species specific protein. The gene of this protein was cloned and a recombinant protein was expressed in E. coli. In addition, an antibody to the recombinant protein was tested in each individual patient using Western blot analysis. None of the forty non-gastric cancer patients were positive for the antibody to the recombinantly expressed 26 kD species specific protein. Meanwhile, six of the twenty four cancer patients tested positive (0/40 vs 6/24, p < 0.01). Results presented herein demonstrate that the species specific protein of H. pylori can be useful in detecting H. pylori associated with adenocarcinoma of the stomach.
Wang
J T
JT
Department of Bacteriology, College of Medicine, National Taiwan University, Taipei, Taiwan. wangjt@ccms.ntu.edu.tw
Chang
C S
CS
Lee
C Z
CZ
Yang
J C
JC
Lin
J T
JT
Wang
T H
TH
D000230:Adenocarcinoma / Q000209:etiology / Q000276:immunology* / Q000382:microbiology*; D000595:Amino Acid Sequence; D000907:Antibodies, Bacterial; D000942:Antigens, Bacterial / Q000235:genetics; D001483:Base Sequence; D003001:Cloning, Molecular; D017931:DNA Primers / Q000235:genetics; D004269:DNA, Bacterial / Q000235:genetics; D004926:Escherichia coli / Q000235:genetics; D005798:Genes, Bacterial; D016481:Helicobacter Infections / Q000150:complications / Q000276:immunology / Q000382:microbiology; D016480:Helicobacter pylori / Q000235:genetics / Q000276:immunology* / Q000472:pathogenicity; D006801:Humans; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D011994:Recombinant Proteins / Q000235:genetics / Q000276:immunology; D013274:Stomach Neoplasms / Q000209:etiology / Q000276:immunology* / Q000382:microbiology*; D014774:Virulence
D000907:Antibodies, Bacterial; D000942:Antigens, Bacterial; D017931:DNA Primers; D004269:DNA, Bacterial; D011994:Recombinant Proteins