title: Studies of the nucleopolyhedrovirus infection process in insects by using the green fluorescence protein as a reporter.
abstract: A recombinant Autographa californica multicapsid nucleopolyhedrovirus (AcMNPV) expressing the green fluorescence protein (GFP) under the control of the AcMNPV polyhedrin promoter was constructed to study the spatial and temporal regulation of baculovirus infection in a permissive host. Larvae that ingested AcMNPV-GFP showed localized expression of GFP in the midgut epithelial cells, as well as hemocytes, at 24 h postinfection. The presence of fluorescence in these tissues indicated not only that the virus was replicating but also that the very late viral proteins were being synthesized. Secondary infection occurred within the tracheal cells throughout the body cavity, confirming earlier reports, and these foci of infection allowed entry of the virus into other tissues, such as the epidermis and the fat body.
authors.affiliation: Great Lakes Forestry Centre, Canadian Forest Service, Sault Ste. Marie, Ontario, Canada.
authors.forename: J W
authors.initials: JW
authors.lastname: Barrett
authors.forename: A J
authors.initials: AJ
authors.lastname: Brownwright
authors.forename: M J
authors.initials: MJ
authors.lastname: Primavera
authors.forename: S R
authors.initials: SR
authors.lastname: Palli
mesh_terms: D000818:Animals; D002460:Cell Line; D017930:Genes, Reporter; D049452:Green Fluorescent Proteins; D008164:Luminescent Proteins / Q000235:genetics / Q000378:metabolism*; D009036:Moths / Q000821:virology*; D017924:Nucleopolyhedroviruses / Q000502:physiology*; D018411:Spodoptera / Q000166:cytology; D013997:Time Factors; D014018:Tissue Distribution
chemical_list: D008164:Luminescent Proteins; D049452:Green Fluorescent Proteins