title: A hirudin-sensitive, growth-related proteinase from human fibroblasts.
abstract: We have identified a hirudin-sensitive proteinase from human fibroblasts. Inhibition of this enzyme results in partial inhibition of cell growth in culture. The enzyme has a molecular weight of about 38000, and can be isolated by affinity chromatography with a hirudin-agarose matrix. The enzyme is apparently not closely related to thrombin, but does show a high level of amino acid sequence homology with fragments of a protein isolated from the synovial fluid of rheumatoid arthritis patients, which acts as a mitogen for human T lymphocytes.
authors.affiliation: School of Biological Sciences, University of Auckland, New Zealand.
authors.forename: D
authors.initials: D
authors.lastname: Bush
authors.forename: H
authors.initials: H
authors.lastname: Fritz
authors.forename: C
authors.initials: C
authors.lastname: Knight
authors.forename: J
authors.initials: J
authors.lastname: Mount
authors.forename: K
authors.initials: K
authors.lastname: Scott
mesh_terms: D000595:Amino Acid Sequence; D010450:Endopeptidases / Q000187:drug effects / Q000302:isolation & purification / Q000378:metabolism*; D005347:Fibroblasts / Q000201:enzymology*; D006629:Hirudins / Q000494:pharmacology*; D006801:Humans; D008969:Molecular Sequence Data; D011480:Protease Inhibitors / Q000494:pharmacology*
chemical_list: D006629:Hirudins; D011480:Protease Inhibitors; D010450:Endopeptidases; C112045:hirudin-sensitive fibroblast proteinase