Peptide mimics of the CTLA4-binding domain stimulate T-cell proliferation.
Phage library clones selected by a conformational epitope-recognizing and inhibitory monoclonal antibody may display moieties that mimic a receptor/ligand-like three-dimensional structure. This pseudoreceptor/ligand should be able to bind to natural ligand/receptor molecules. We tested this idea using anti-T cell costimulatory molecule antibodies and successfully isolated phage clones with costimulatory effects on T-cell proliferation. This strategy facilitates the designing of regulatory peptide molecules in the absence of precise information about the structure-function relationships in receptor/ligand interactions.
Fukumoto
T
T
Department of Bioengineering, Faculty of Engineering, Kagoshima University, Japan.
Torigoe
N
N
Kawabata
S
S
Murakami
M
M
Uede
T
T
Nishi
T
T
Ito
Y
Y
Sugimura
K
K
D000069594:Abatacept; D000595:Amino Acid Sequence; D000818:Animals; D000911:Antibodies, Monoclonal; D015703:Antigens, CD / Q000276:immunology / Q000378:metabolism; D000943:Antigens, Differentiation / Q000276:immunology*; D018122:B7-1 Antigen / Q000276:immunology / Q000378:metabolism; D051940:B7-2 Antigen; D001435:Bacteriophages / Q000235:genetics / Q000276:immunology; D060908:CTLA-4 Antigen; D002455:Cell Division / Q000187:drug effects; D006224:Cricetinae; D000939:Epitopes; D018796:Immunoconjugates; D008562:Membrane Glycoproteins / Q000276:immunology / Q000378:metabolism; D051379:Mice; D008807:Mice, Inbred BALB C; D018448:Models, Immunological; D008969:Molecular Sequence Data; D010455:Peptides / Q000276:immunology* / Q000378:metabolism* / Q000494:pharmacology; D013601:T-Lymphocytes / Q000187:drug effects / Q000276:immunology*
D000911:Antibodies, Monoclonal; D015703:Antigens, CD; D000943:Antigens, Differentiation; D018122:B7-1 Antigen; D051940:B7-2 Antigen; D060908:CTLA-4 Antigen; C497965:Cd86 protein, mouse; C556707:Ctla4 protein, mouse; D000939:Epitopes; D018796:Immunoconjugates; D008562:Membrane Glycoproteins; D010455:Peptides; D000069594:Abatacept