title: Dot immunobinding assay for detection of mite antigens in house-dust samples.
abstract: A new test was developed specifically to detect mite antigens in house-dust. It uses a nitrocellulose dipstick spotted with specific antimite antibodies that act as a capture matrix; the same antibodies act as a detecting reagent when conjugated with colloidal dye particles. Aclotest is a 1-step assay, where a spotted dipstick is placed in a tube containing the detecting reagent and the house-dust sample. No instrumentation or previous extraction procedure of the sample is required, and the test response is visible as a colored spot, after 1 h incubation. The sensitivity and specificity of the new test were compared with those of Acarex and Der p1/Der f1 ELISA tests.
authors.affiliation: Research Department, Laboratorio Farmaceutico Lofarma, Milan, Italy.
authors.forename: G
authors.initials: G
authors.lastname: Mistrello
authors.forename: M
authors.initials: M
authors.lastname: Gentili
authors.forename: D
authors.initials: D
authors.lastname: Roncarolo
authors.forename: P
authors.initials: P
authors.lastname: Antoniotti
authors.forename: F
authors.initials: F
authors.lastname: Ottoboni
authors.forename: P
authors.initials: P
authors.lastname: Falagiani
mesh_terms: D000818:Animals; D000918:Antibody Specificity; D000941:Antigens / Q000032:analysis*; D004391:Dust / Q000032:analysis*; D006798:Housing; D006801:Humans; D008925:Mites
chemical_list: D000941:Antigens; D004391:Dust