Hydrogen exchange and protein folding.
Amide hydrogen-deuterium exchange is a sensitive probe of the structure, stability and dynamics of proteins. The significant increase in the number of small, model proteins that have been studied has allowed a better understanding of the structural fluctuations that lead to hydrogen exchange. Recent technical advances enable the methodology to be applied to the study of protein-protein interactions in much larger, more complex systems.
Clarke
J
J
Centre for Protein Engineering, MRC Unit for Protein Function and Design, MRC Centre, Cambridge, UK. jc162@cam.ac.uk
Itzhaki
L S
LS
D006859:Hydrogen / Q000737:chemistry*; D006860:Hydrogen Bonding; D008956:Models, Chemical; D011487:Protein Conformation; D017510:Protein Folding; D011506:Proteins / Q000737:chemistry* / Q000378:metabolism; D013696:Temperature
D011506:Proteins; D006859:Hydrogen