Molecular cloning and nucleotide sequence of a cytochrome P450 cDNA from a pyrethroid-resistant mosquito, Culex quinquefasciatus say.
A novel cDNA clone encoding a cytochrome P450 was screened from a gut cDNA library of permethrin-resistant Culex quinquefasciatus larvae. Nucleotide sequence of the clone designated CYP6E1 was determined. This is the first full-length sequence of a mosquito P450 cDNA. Comparison of the deduced amino acid sequence of CYP6E1 to those of other known cytochrome P450s indicates that this clone belongs to CYP6 family. In addition, a phylogenetic analysis of CYP6E1 primary structure showed that it is related to CYP6A and CYP6C subfamilies. Polymerase chain reaction (PCR) using primers designed on the basis of the nucleotide sequence of the cDNA showed that mRNA encoding CYP6E1 is present in permethrin-susceptible larvae as well as -resistant larvae, suggesting the ubiquitous distribution of CYP6E1.
Kasai
S
S
Laboratory of Applied Zoology, Institute of Agriculture and Forestry, University of Tsukuba, Japan.
Shono
T
T
Yamakawa
M
M
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D003465:Culex / Q000201:enzymology* / Q000235:genetics; D003577:Cytochrome P-450 Enzyme System / Q000235:genetics*; D018076:DNA, Complementary; D007305:Insecticide Resistance; D008969:Molecular Sequence Data; D011722:Pyrethrins; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid
D018076:DNA, Complementary; D011722:Pyrethrins; D003577:Cytochrome P-450 Enzyme System