title: Submucosa 1.0 x 0.1 mm in size is sufficient to count inflammatory cell numbers in human airway biopsy specimens.
abstract: Counting of inflammatory cells in human airway biopsy specimens is difficult because immunopositive cells are present in varying density in lung tissue. The goal of our study was to assess the minimal amount of tissue that is necessary for the counting of constant cell numbers. In bronchial biopsy specimens from 5 healthy controls and 5 patients with asthma, we evaluated 20 successive areas of submucosa 0.1 x 0.1 mm in size. We recorded positive and negative changes of more than 10% in the counted numbers of CD4-, CD8-, and EG2-positive cells. We demonstrated that tissue 1.0 x 0.1 mm in size, along 1-mm basement membrane, is sufficient to obtain constant cell numbers.
authors.affiliation: Department of Pulmonology, University Hospital Groningen, The Netherlands.
authors.forename: N H
authors.initials: NH
authors.lastname: ten Hacken
authors.forename: R M
authors.initials: RM
authors.lastname: Aleva
authors.forename: Y
authors.initials: Y
authors.lastname: Oosterhoff
authors.forename: M
authors.initials: M
authors.lastname: Smith
authors.forename: J
authors.initials: J
authors.lastname: Kraan
authors.forename: D S
authors.initials: DS
authors.lastname: Postma
authors.forename: W
authors.initials: W
authors.lastname: Timens
mesh_terms: D001249:Asthma / Q000473:pathology / Q000503:physiopathology; D001706:Biopsy; D001798:Blood Proteins / Q000032:analysis; D001980:Bronchi / Q000737:chemistry / Q000166:cytology* / Q000473:pathology*; D001991:Bronchitis / Q000473:pathology* / Q000503:physiopathology; D016516:CD4-CD8 Ratio; D002452:Cell Count / Q000379:methods*; D047091:Eosinophil Granule Proteins; D006801:Humans; D007150:Immunohistochemistry; D018836:Inflammation Mediators / Q000032:analysis; D009092:Mucous Membrane / Q000737:chemistry / Q000166:cytology / Q000503:physiopathology; D012260:Ribonucleases
chemical_list: D001798:Blood Proteins; D047091:Eosinophil Granule Proteins; D018836:Inflammation Mediators; D012260:Ribonucleases