Heteronuclear X-filter 1H PFG double-quantum experiments for the proton resonance assignment of a ligand bound to a protein.
A novel X-filter experiment based on 1H PFG DQ spectroscopy is described. Excellent suppression of proton bound to 13C and 15N is achieved. Successful application of the method to a protein-ligand complex is demonstrated.
Dalvit
C
C
NOVARTIS Pharma AG, Basel, CH-4002, Switzerland.
Ramage
P
P
Hommel
U
U
D000465:Algorithms; D002247:Carbon Isotopes; D004578:Electron Spin Resonance Spectroscopy; D005947:Glucose / Q000737:chemistry; D006859:Hydrogen; D007089:Image Enhancement / Q000379:methods; D008024:Ligands; D016169:Lymphocyte Function-Associated Antigen-1 / Q000737:chemistry*; D009587:Nitrogen Isotopes; D019906:Nuclear Magnetic Resonance, Biomolecular / Q000379:methods*; D010649:Phenylalanine / Q000737:chemistry; D011485:Protein Binding; D011522:Protons
D002247:Carbon Isotopes; D008024:Ligands; D016169:Lymphocyte Function-Associated Antigen-1; D009587:Nitrogen Isotopes; D011522:Protons; D010649:Phenylalanine; D006859:Hydrogen; D005947:Glucose