Identification of a distinct antibacterial domain within the N-lobe of ovotransferrin.
We have evaluated the bactericidal activity of hen ovotransferrin (OTf), which was found to operate regardless of its iron-deprivation properties, with the objective of isolating the bactericidal domain. The amino-terminal half-molecule (N-lobe, residues 1-332) of OTf, isolated by trypsin-nicking, retained the bactericidal activity independently of iron-deprivation, but not the carboxyl-terminal half-molecule (C-lobe, residue 342-686), suggesting the presence of a bactericidal domain within the N-lobe of the molecule. Specific cleavage at the aspartyl residues of OTf, by diluted-acid procedure, yielded fairly large peptides, whereas proteolysis for 150 min produced the strongest bactericidal peptides mixture. The bactericidal domain was purified from the active hydrolysate by gel filtration and reversed-phase HPLC and showed activity against S. aureus as well as E. coli K-12. Electrophoretic analysis on tricine-SDS-PAGE revealed a bactericidal peptide with an average M(r) of 9900 Da under non-reducing conditions. In combination with the specificity of cleavage (Asp-X) and the molecular mass, its N-terminal microsequencing corresponded to a cationic peptide consisting of 92 residues located within the 109-200 sequence of the N-lobe of OTf, containing three intrachain disulfide bridges, featuring a common structural motif occurs in the N-lobes of transferrins for which the sequence is available. Two of the disulfides (C160-C174 and C171-C182) form surface exposed cringle bridges lying on the opposite side of the iron-binding site from the interdomain cleft and showing marked sequence homology to insect defensins, which are blockers of the voltage-dependent K+ channels. The peptide lost antibacterial activity when its disulfide bonds were reduced, indicating the importance of its tertiary structure for the exertion of antibiotic activity.
Ibrahim
H R
HR
Department of Biochemical Science and Technology, Faculty of Agriculture, Kagoshima University, Japan. hishamri@chem.agri.kagoshima-u.ac.jp
Iwamori
E
E
Sugimoto
Y
Y
Aoki
T
T
D000595:Amino Acid Sequence; D000818:Animals; D001224:Aspartic Acid / Q000378:metabolism; D001419:Bacteria / Q000187:drug effects*; D002645:Chickens; D002850:Chromatography, Gel; D002851:Chromatography, High Pressure Liquid; D003207:Conalbumin / Q000737:chemistry* / Q000494:pharmacology*; D006851:Hydrochloric Acid; D007501:Iron; D008826:Microbial Sensitivity Tests; D008969:Molecular Sequence Data; D010446:Peptide Fragments / Q000302:isolation & purification / Q000494:pharmacology; D011487:Protein Conformation; D017386:Sequence Homology, Amino Acid; D013329:Structure-Activity Relationship
D010446:Peptide Fragments; D003207:Conalbumin; D001224:Aspartic Acid; D007501:Iron; D006851:Hydrochloric Acid