title: Conservation of substrate recognition mechanisms by tRNA splicing endonucleases.
abstract: Accuracy in transfer RNA (tRNA) splicing is essential for the formation of functional tRNAs, and hence for gene expression, in both Eukaryotes and Archaea. The specificity for recognition of the tRNA precursor (pre-tRNA) resides in the endonuclease, which removes the intron by making two independent endonucleolytic cleavages. Although the eukaryal and archaeal enzymes appear to use different features of pre-tRNAs to determine the sites of cleavage, analysis of hybrid pre-tRNA substrates containing eukaryal and archaeal sequences, described here, reveals that the eukaryal enzyme retains the ability to use the archaeal recognition signals. This result indicates that there may be a common ancestral mechanism for recognition of pre-tRNA by proteins.
authors.affiliation: EniChem, Istituto Guido Donegani SpA, Laboratori di Biotecnologie, 00015 Monterotondo, Rome, Italy.
authors.forename: S
authors.initials: S
authors.lastname: Fabbri
authors.forename: P
authors.initials: P
authors.lastname: Fruscoloni
authors.forename: E
authors.initials: E
authors.lastname: Bufardeci
authors.forename: E
authors.initials: E
authors.lastname: Di Nicola Negri
authors.forename: M I
authors.initials: MI
authors.lastname: Baldi
authors.forename: D G
authors.initials: DG
authors.lastname: Attardi
authors.forename: E
authors.initials: E
authors.lastname: Mattoccia
authors.forename: G P
authors.initials: GP
authors.lastname: Tocchini-Valentini
mesh_terms: D000818:Animals; D000926:Anticodon; D001482:Base Composition; D001483:Base Sequence; D004722:Endoribonucleases / Q000737:chemistry / Q000378:metabolism*; D007438:Introns; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation; D012322:RNA Precursors / Q000737:chemistry* / Q000378:metabolism*; D012326:RNA Splicing; D019642:RNA, Archaeal / Q000737:chemistry* / Q000378:metabolism*; D012360:RNA, Transfer, Phe / Q000737:chemistry / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000201:enzymology; D013379:Substrate Specificity; D014981:Xenopus
chemical_list: D000926:Anticodon; D012322:RNA Precursors; D019642:RNA, Archaeal; D012360:RNA, Transfer, Phe; D004722:Endoribonucleases; C051012:splicing endonuclease