Long-term prognostic value of PCNA labeling index in primary operable breast cancer.
Cell proliferation was evaluated in 167 tissue specimens obtained from primary breast cancer patients who had undergone radical surgery between 1984 and 1988. Formalin-fixed and paraffin-embedded tissue specimens were used in the immunohistochemical study. The immunohistochemical method was carried out using the avidin-biotin immunoperoxidase technique, and anti-proliferating cell nuclear antigen (PCNA) monoclonal antibody was used for primary antibody. Based upon the PCNA labeling index (LI), the patients were divided into two groups: low PCNA, <25% and high PCNA, >/= 25%. The PCNA LI ranged from 1% to 76% (mean, 23.9%). Patients aged </= 50 showed significantly higher PCNA LI than those of > 50 years. There was no relationship between the PCNA LI and tumor size, lymph node involvement and hormone receptors. The survival curves of 146 invasive breast cancer patients showed that the high PCNA group had poor overall survival compared with the low PCNA group. A significant difference in the overall survival between the high and low PCNA groups was observed in lymph node-positive patients, however, no significant difference was found between the two groups in lymph node-negative patients. PCNA LI was identified as an independent predictor in primary breast cancer patients.
Horiguchi
J
J
Second Department of Surgery, Gunma University School of Medicine, 3-39-15 Showa-Machi, Maebashi, Gunma, 371, Japan.
Iino
Y
Y
Takei
H
H
Maemura
M
M
Takeyoshi
I
I
Yokoe
T
T
Ohwada
S
S
Oyama
T
T
Nakajima
T
T
Morishita
Y
Y
D000328:Adult; D001943:Breast Neoplasms / Q000378:metabolism* / Q000401:mortality / Q000601:surgery; D018270:Carcinoma, Ductal, Breast / Q000378:metabolism* / Q000401:mortality / Q000601:surgery; D002455:Cell Division; D018572:Disease-Free Survival; D005260:Female; D005500:Follow-Up Studies; D006801:Humans; D007150:Immunohistochemistry; D008207:Lymphatic Metastasis; D015409:Mastectomy, Radical; D008875:Middle Aged; D008940:Mitotic Index; D009364:Neoplasm Recurrence, Local; D010144:Paget's Disease, Mammary / Q000378:metabolism* / Q000401:mortality / Q000601:surgery; D011379:Prognosis; D018809:Proliferating Cell Nuclear Antigen / Q000378:metabolism*; D011960:Receptors, Estrogen / Q000378:metabolism; D011980:Receptors, Progesterone / Q000378:metabolism; D012189:Retrospective Studies; D015996:Survival Rate
D018809:Proliferating Cell Nuclear Antigen; D011960:Receptors, Estrogen; D011980:Receptors, Progesterone