title: Stimulatory effect of lymphocyte-derived factor on catecholamine efflux from cultured bovine adrenal medullary cells.
abstract: The effects of lymphocytes and their conditioned medium on catecholamine efflux and uptake were examined in cultured bovine adrenal medullary cells. Co-culture of adrenal medullary cells with lymphocytes for 3 days caused an increase in appearance of catecholamines in the culture medium. Treatment of adrenal medullary cells with a conditioned medium prepared from lymphocytes also enhanced the appearance of catecholamines in culture medium in time- (8-48 h) and concentration-dependent manners. Heat treatment of the conditioned medium at 60 and 100 degrees C for 10 min reduced its stimulatory effect to 59 and 20% of control, respectively. After gel filtration on a Sephadex G-25 column or dialysis (<8 kDa molecular mass cutoff), the stimulatory activity of the conditioned medium was found in a high molecular fraction. The conditioned medium had little effect on the activity of lactate dehydrogenase in the medium of cultured adrenal medullary cells and on desipramine-sensitive [3H]norepinephrine uptake by the cells. These findings suggest that lymphocytes release a heat-sensitive factor(s) (molecular mass of more than 8 kDa) which increases efflux of catecholamines from cultured adrenal medullary cells.
authors.affiliation: Department of Pharmacology, University of Occupational and Environmental Health, School of Medicine, Kitakyushu, Japan.
authors.forename: N
authors.initials: N
authors.lastname: Yanagihara
authors.forename: K
authors.initials: K
authors.lastname: Hara
authors.forename: K
authors.initials: K
authors.lastname: Kajiwara
authors.forename: K
authors.initials: K
authors.lastname: Minami
authors.forename: Y
authors.initials: Y
authors.lastname: Toyohira
authors.forename: Y
authors.initials: Y
authors.lastname: Uezono
authors.forename: S
authors.initials: S
authors.lastname: Ueno
authors.forename: H
authors.initials: H
authors.lastname: Hirano
authors.forename: U
authors.initials: U
authors.lastname: Yamashita
authors.forename: F
authors.initials: F
authors.lastname: Izumi
mesh_terms: D000313:Adrenal Medulla / Q000166:cytology* / Q000187:drug effects; D018759:Adrenergic Uptake Inhibitors / Q000494:pharmacology; D000818:Animals; D002417:Cattle; D002478:Cells, Cultured; D017077:Culture Media, Conditioned / Q000494:pharmacology*; D003891:Desipramine / Q000494:pharmacology; D006358:Hot Temperature; D008214:Lymphocytes / Q000166:cytology / Q000378:metabolism*; D008222:Lymphokines / Q000378:metabolism; D009638:Norepinephrine / Q000378:metabolism* / Q000493:pharmacokinetics; D013566:Sympathomimetics / Q000378:metabolism / Q000493:pharmacokinetics; D014316:Tritium
chemical_list: D018759:Adrenergic Uptake Inhibitors; D017077:Culture Media, Conditioned; D008222:Lymphokines; D013566:Sympathomimetics; D014316:Tritium; D003891:Desipramine; D009638:Norepinephrine