Macrophage-colony stimulating factor (M-CSF) regulates the expression of fibronectin and its alpha5 integrin receptor in human trophoblasts.
Macrophage-colony stimulating factor (M-CSF, CSF-1) is secreted by human trophoblasts as well as endometrial cells, while its receptor c-fms is abundantly expressed by the extravillous trophoblasts anchoring the placenta to the uterus, suggesting a role for M-CSF at the maternal-fetal interface. We investigated the effect of M-CSF on the expression of fibronectin and its receptor, the alpha5 integrin, in human trophoblasts. Exposure of trophoblasts to M-CSF produced a two to three-fold increase in fibronectin and alpha5 mRNA abundance at both 24 and 72 hours of culture. A dose-dependent increase in cellular fibronectin secretion into the culture medium was detected at both time points. Immunocytochemistry showed co-localization of cellular fibronectin and alpha5 in the cells, suggesting that attachment of trophoblasts to fibronectin is mediated in part by the alpha5beta1 integrin. We conclude that M-CSF increases fibronectin expression and secretion by the human trophoblasts, and up-regulates its specific receptor, the alpha5 integrin. We hypothesize that M-CSF may partake in the autocrine/paracrine mechanisms regulating trophoblast invasion during implantation.
Omigbodun
A
A
Department of Obstetrics and Gynecology, and the Center for Research in Reproduction and Women's Health, University of Pennsylvania, Philadelphia 19104, USA.
Coukos
G
G
Ziolkiewicz
P
P
Wang
C L
CL
Coutifaris
C
C
D015703:Antigens, CD / Q000032:analysis / Q000235:genetics*; D002478:Cells, Cultured; D010064:Embryo Implantation; D005260:Female; D005353:Fibronectins / Q000032:analysis / Q000235:genetics*; D019084:Fluorescent Antibody Technique, Indirect; D005786:Gene Expression Regulation; D006801:Humans; D039564:Integrin alphaV; D016173:Macrophage Colony-Stimulating Factor / Q000494:pharmacology*; D011247:Pregnancy; D012333:RNA, Messenger / Q000096:biosynthesis; D014327:Trophoblasts / Q000737:chemistry / Q000378:metabolism*
D015703:Antigens, CD; D005353:Fibronectins; D039564:Integrin alphaV; D012333:RNA, Messenger; D016173:Macrophage Colony-Stimulating Factor