In-vitro activities of paromomycin and lasalocid evaluated in combination against Cryptosporidium parvum.
Using a chemiluminescence immunoassay, paromomycin and lasalocid were shown to inhibit Cryptosporidium parvum growth in Madin-Darby canine kidney cells in a concentration-dependent manner. The median effective concentrations (EC50s) for paromomycin and lasalocid were 1184 mg/L and 0.4 mg/L, respectively. Neither drug was cytotoxic to host cells at concentrations up to five times their EC50s. Drug combination studies were conducted and the resulting data were analysed by the median-effect principle and combination index method. Statistically significant synergy was observed when combinations of paromomycin and lasalocid were used at ratios of 5000:1 and 2500:1. A possible mechanism for synergy is discussed.
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Department of Pediatrics, Emory University School of Medicine, Atlanta, GA 30322, USA.
Schinazi
R F
RF
Arrowood
M J
MJ
Lejkowski
M
M
Juodawlkis
A S
AS
Mead
J R
JR
D000818:Animals; D002417:Cattle; D016785:Cryptosporidium parvum / Q000187:drug effects* / Q000254:growth & development; D004285:Dogs; D004305:Dose-Response Relationship, Drug; D004359:Drug Therapy, Combination; D005434:Flow Cytometry; D007118:Immunoassay / Q000379:methods; D007668:Kidney / Q000166:cytology / Q000469:parasitology; D007832:Lasalocid / Q000494:pharmacology* / Q000633:toxicity; D008163:Luminescent Measurements; D010303:Paromomycin / Q000494:pharmacology* / Q000633:toxicity; D011419:Propidium / Q000493:pharmacokinetics
D011419:Propidium; D010303:Paromomycin; D007832:Lasalocid