title: In vitro gene transfer in mammalian cells via a new cationic liposome formulation.
abstract: A new cationic liposome formulation of sphingosine (SP) and dioleoylphosphatidylethanolamine (DOPE) was developed as an efficient transfection reagent. This SP/DOPE liposome showed efficient transfection in a wide variety of mammalian cancer cells. No significant cytotoxicity of the SP/DOPE liposome to cells was observed. The tranfection activity was greater than that of a well-reported liposome which was made from a cholesterol derivative 3beta-[N-(N',N'-dimethylaminoethane)-carbamoyl] cholesterol (DC-Chol) and the neutral lipid DOPE. In addition, the SP/DOPE liposome was found to be less toxic to cells than the DC-Chol/DOPE liposome. Stable transfections mediated by SP/ DOPE liposome were also demonstrated. These results suggest that the SP/DOPE liposome may provide a good gene delivery system to be used in the human cancer gene therapy.
authors.affiliation: Department of Biochemistry, National Defense Medical Center, P.O. Box 90048-501, Taipei, 100, Taiwan, R.O.C.
authors.forename: M C
authors.initials: MC
authors.lastname: Kao
authors.forename: S L
authors.initials: SL
authors.lastname: Law
authors.forename: T C
authors.initials: TC
authors.lastname: Chuang
authors.forename: Y S
authors.initials: YS
authors.lastname: Lin
mesh_terms: D016475:3T3 Cells; D000818:Animals; D015153:Blotting, Western; D002470:Cell Survival / Q000187:drug effects; D002784:Cholesterol / Q000031:analogs & derivatives / Q000737:chemistry; D005260:Female; D018734:Genes, erbB-2 / Q000235:genetics*; D006801:Humans; D008081:Liposomes / Q000737:chemistry*; D051379:Mice; D009369:Neoplasms / Q000235:genetics*; D010714:Phosphatidylethanolamines / Q000737:chemistry*; D010957:Plasmids; D013110:Sphingosine / Q000737:chemistry*; D014162:Transfection / Q000379:methods*; D014407:Tumor Cells, Cultured; D001616:beta-Galactosidase / Q000235:genetics
chemical_list: D008081:Liposomes; D010714:Phosphatidylethanolamines; C070648:3-(N-(N',N'-dimethylaminoethane)carbamoyl)cholesterol; C020888:1,2-dielaidoylphosphatidylethanolamine; D002784:Cholesterol; D001616:beta-Galactosidase; D013110:Sphingosine