Effects of vertebrate growth factors on digestive gland cells from the mollusc Pecten maximus L.: an in vitro study.
In relation with the digestive cycle, the digestive gland cells of bivalve molluscs undergo a sequence of cytological changes which is controlled by external and internal effectors such as putative gastrointestinal hormones and growth differentiation factors. A tissue dissociation method was developed to investigate the in vitro effect of the vertebrate growth and differentiation factors: insulin, insulin growth factor I (IGF-I), basic fibroblast growth factor (bFGF) and epidermal growth factor (EGF) on the digestive gland cells of the scallop Pecten maximus. All these vertebrate peptides induced a dose-dependent increased incorporation of 3H-leucine and 14C-uridine in whole digestive gland cell suspensions. However, after Percoll density gradient purification of the digestive cells, only stem and undifferentiated enriched cell fractions were responsive to the different peptides. In addition, insulin and IGF-I, but not EGF and bFGF, stimulated 3H-leucine incorporation in control dispersed mantle edge cells. These results suggest that insulin-related peptides could work as general growth promoting factors in molluscs. On the other hand, EGF and bFGF, or at least their molluscan counterparts, may be efficient growth differentiation factors in the regenerative processes occurring in the digestive gland of molluscs.
Giard
W
W
Laboratoire de Biologie et Biotechnologies Marines, IBBA, IFREMER URM 14, Université de Caen, France.
Lebel
J M
JM
Boucaud-Camou
E
E
Favrel
P
P
D000818:Animals; D002454:Cell Differentiation; D002478:Cells, Cultured; D004064:Digestive System / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D004815:Epidermal Growth Factor / Q000494:pharmacology*; D016222:Fibroblast Growth Factor 2 / Q000494:pharmacology*; D006801:Humans; D066298:In Vitro Techniques; D007328:Insulin / Q000494:pharmacology; D007334:Insulin-Like Growth Factor I / Q000494:pharmacology*; D007930:Leucine / Q000378:metabolism; D008974:Mollusca / Q000187:drug effects* / Q000502:physiology; D014176:Protein Biosynthesis; D012313:RNA / Q000096:biosynthesis; D014529:Uridine / Q000378:metabolism
D007328:Insulin; D016222:Fibroblast Growth Factor 2; D004815:Epidermal Growth Factor; D012313:RNA; D007334:Insulin-Like Growth Factor I; D007930:Leucine; D014529:Uridine