[In vitro inhibition of the growth of Gardnerella vaginalis by bacteriocins produced by strains of Pseudomonas aeruginosa].
BACKGROUND
Pseudomonas aeruginosa with inhibitory capacity in vitro was studied on Gardnerella vaginalis strains.


METHODS
Antimicrobial activity was demonstrated by inhibitory halos of bacterial growth on solid media by two methods: crossed streak and agar well diffusion. The inhibitory activity of this substance produced by P. aeruginosa was characterized as bacteriocin by: activity spectrum sensitivity proteolytic enzyme, chloroform, heat, pH, ultraviolet, irradiation effect and molecular weight.


RESULTS
Four strains of P. aeruginosa producers of bacteriocins were chosen for this study and contacted with 40 strains of G. vaginalis. The producing strain D inhibited 70% of these G. vaginalis strains. The strains B and C inhibited 55% and 52.5%, respectively. The 3 strains presented a wide rank of action but the strain A had effect on a few strains of G. vaginalis.


CONCLUSIONS
This work showed the inhibitory in vitro effect of bacteriocins of P. aeruginosa on strains of G. vaginalis. The results obtained suggest the probable topic application of bacteriocins as an alternative of conventional therapeutic on this infection biological control.
Barberis
I L
IL
Departamento de Microbiología e Inmunología, Universidad Nacional de Río Cuarto, Córdoba, Argentina.
Pájaro
M C
MC
Godino
S D
SD
Aichino
C
C
D001430:Bacteriocins / Q000378:metabolism / Q000494:pharmacology*; D005260:Female; D006194:Gardnerella vaginalis / Q000187:drug effects* / Q000254:growth & development / Q000302:isolation & purification; D006801:Humans; D008826:Microbial Sensitivity Tests; D011550:Pseudomonas aeruginosa / Q000378:metabolism*; D013045:Species Specificity; D016585:Vaginosis, Bacterial / Q000382:microbiology
D001430:Bacteriocins
Inhibición in vitro del crecimiento de Gardnerella vaginalis por bacteriocinas producidas por cepas de Pseudomonas aeruginosa.