A strain-type clustering of potato virus Y based on the genetic distance between isolates calculated by RFLP analysis of the amplified coat protein gene.
Potato virus Y (PVY) isolates have been classified into genetic strains by a host-independent criterion using a molecular typing method. The method used extracts from infected tissue, and included immunocapture-RT-PCR-RFLP analysis using 5 different restriction endonucleases (Dde I, Eco RV, Hinf I, Rsa I and Taq I). Genetic distances between the different PVY "restrictotypes" were calculated and used to define the PVY genetic strains. Three main clusters were found: PVYO, PVYN, and non-potato PVY (PVYNP), in good agreement with classical PVY strain definitions that combine different biological criteria. Our approach was incomparably quicker and more reliable and reproducible than biotyping. The potential of this approach for very quick, simple and automatable molecular epidemiological studies is discussed.
Blanco-Urgoiti
B
B
Laboratorio de Biología Molecular y Virología Vegetal, CIT-INIA, Madrid, Spain.
Sánchez
F
F
Dopazo
J
J
Ponz
F
F
D000911:Antibodies, Monoclonal / Q000276:immunology; D000914:Antibodies, Viral / Q000276:immunology; D002213:Capsid / Q000235:genetics* / Q000276:immunology; D004279:DNA, Viral / Q000032:analysis* / Q000235:genetics; D005814:Genes, Viral; D016679:Genome, Viral; D005838:Genotype; D010802:Phylogeny; D010947:Plants, Toxic; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length; D017800:Potyvirus / Q000145:classification* / Q000235:genetics* / Q000276:immunology; D014026:Nicotiana / Q000821:virology
D000911:Antibodies, Monoclonal; D000914:Antibodies, Viral; D004279:DNA, Viral