Expression of HuD protein is essential for initial phase of neuronal differentiation in rat pheochromocytoma PC12 cells.
HuD is a neuronal-specific, RNA-binding protein. Here we examined the change in the expression of HuD protein during nerve growth factor-mediated differentiation of PC12 cells. As cells differentiated and extended neurites, expression of HuD gradually increased up to 1.5-fold. When HuD expression was counteracted by antisense oligonucleotide, neurite extension was completely inhibited, yet the morphology of differentiated cells remained unchanged even after that treatment. Furthermore, this morphological change correlated well with the downregulation of cyclin-dependent kinase 2 activity. These results suggest that the HuD is critically involved in the initial phase of neuronal differentiation.
Dobashi
Y
Y
Department of Pathology, Kitasato University School of Medicine, Kanagawa, Japan.
Shoji
M
M
Wakata
Y
Y
Kameya
T
T
D000818:Animals; D042846:CDC2-CDC28 Kinases; D002454:Cell Differentiation / Q000187:drug effects; D048429:Cell Size / Q000187:drug effects; D051357:Cyclin-Dependent Kinase 2; D018844:Cyclin-Dependent Kinases / Q000378:metabolism; D051959:ELAV Proteins; D006131:Growth Inhibitors / Q000494:pharmacology; D009414:Nerve Growth Factors / Q000494:pharmacology; D009419:Nerve Tissue Proteins; D016501:Neurites / Q000187:drug effects / Q000502:physiology; D009474:Neurons / Q000166:cytology* / Q000187:drug effects / Q000201:enzymology; D016376:Oligonucleotides, Antisense / Q000494:pharmacology; D016716:PC12 Cells; D017346:Protein Serine-Threonine Kinases / Q000378:metabolism; D016601:RNA-Binding Proteins / Q000096:biosynthesis* / Q000235:genetics / Q000502:physiology; D051381:Rats
D051959:ELAV Proteins; D006131:Growth Inhibitors; D009414:Nerve Growth Factors; D009419:Nerve Tissue Proteins; D016376:Oligonucleotides, Antisense; D016601:RNA-Binding Proteins; D017346:Protein Serine-Threonine Kinases; D042846:CDC2-CDC28 Kinases; C495892:Cdk2 protein, rat; D051357:Cyclin-Dependent Kinase 2; D018844:Cyclin-Dependent Kinases