Haemolytic activity of Mycobacterium spp.
Haemolytic activity of clinical isolates of Mycobacterium bacilli (98) was determined by the method of King et al., 1993. During 3-h incubation, all M. tuberculosis (MTB) isolates (28) and one out of 38 M. avium-intracellulare (MAI) strains, produced a strong contact-dependent haemolysin (CDH). Six MAI strains expressed a weak CDH. One MAI isolate produced a strong and five other MAI strains a weak contact-independent haemolysin (CIH). Two M. bovis BCG strains and 7 M. vaccae strains did not demonstrate haemolytic activity. The persistence of chosen Mycobacterium strains differing by haemolytic activity, in the spleens of infected C57BL/6 mice was examined. Mycobacteria producing a strong CDH (MTB H37Rv, MTB 101/92, MAI 83/93) or CIH (MAI 475/93) survived in the spleens of nonimmunized or M. bovis BCG-immunized mice for longer time than MAI strains expressing weak haemolytic activity or M. bovis BCG vaccine strain.
Brzychcy
M
M
Department of Infectious Biology, University of Lódź, Poland.
Andrzejczyk
Z
Z
Zalewska
N
N
Zwolska
Z
Z
Rudnicka
W
W
D000818:Animals; D001500:BCG Vaccine / Q000276:immunology; D006460:Hemolysin Proteins / Q000096:biosynthesis*; D006461:Hemolysis; D006801:Humans; D051379:Mice; D008810:Mice, Inbred C57BL; D009161:Mycobacterium / Q000378:metabolism* / Q000472:pathogenicity / Q000502:physiology; D015269:Mycobacterium avium Complex / Q000378:metabolism / Q000472:pathogenicity / Q000502:physiology; D015270:Mycobacterium avium-intracellulare Infection / Q000382:microbiology; D009163:Mycobacterium bovis / Q000378:metabolism / Q000472:pathogenicity / Q000502:physiology; D009169:Mycobacterium tuberculosis / Q000378:metabolism / Q000472:pathogenicity / Q000502:physiology; D013045:Species Specificity; D013053:Spectrophotometry; D013154:Spleen / Q000382:microbiology; D014376:Tuberculosis / Q000382:microbiology; D014774:Virulence
D001500:BCG Vaccine; D006460:Hemolysin Proteins