title: The olfactory adenylyl cyclase type 3 is expressed in male germ cells.
abstract: Elements of the olfactory pathway, such as receptors, receptor-desensitization machinery, and cyclic nucleotide-gated channels, are expressed in male germ cells. Here we report the expression, in rat testis, of both adenylyl cyclase type 3 (AC3) and the olfactory G protein subunit, G(alpha)olf. Both are expressed in the same sub-population of germ cells, pachytene spermatocytes to spermatids, and in residual bodies. Neither AC3 nor G(alpha)olf was found in Sertoli or in peritubular cells, as shown by Western blotting and immunocytochemical analyses. It thus appears that male germ cells contain all the elements of the signaling cascade present in olfactory cells.
authors.affiliation: U-99 INSERM, Hopital Henri Mondor, Créteil, France.
authors.forename: N
authors.initials: N
authors.lastname: Defer
authors.forename: O
authors.initials: O
authors.lastname: Marinx
authors.forename: M
authors.initials: M
authors.lastname: Poyard
authors.forename: M O
authors.initials: MO
authors.lastname: Lienard
authors.forename: B
authors.initials: B
authors.lastname: Jégou
authors.forename: J
authors.initials: J
authors.lastname: Hanoune
mesh_terms: D000262:Adenylyl Cyclases / Q000235:genetics / Q000276:immunology / Q000378:metabolism*; D000595:Amino Acid Sequence; D000818:Animals; D002454:Cell Differentiation; D044385:GTP-Binding Protein alpha Subunits; D019204:GTP-Binding Proteins / Q000276:immunology / Q000378:metabolism*; D020962:Heterotrimeric GTP-Binding Proteins; D007150:Immunohistochemistry; D008297:Male; D008969:Molecular Sequence Data; D009830:Olfactory Bulb / Q000378:metabolism; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D015398:Signal Transduction; D013087:Spermatids / Q000378:metabolism; D013094:Spermatozoa / Q000201:enzymology*
chemical_list: D044385:GTP-Binding Protein alpha Subunits; D012333:RNA, Messenger; C084618:olfactory G protein subunit alpha olf; D019204:GTP-Binding Proteins; D020962:Heterotrimeric GTP-Binding Proteins; D000262:Adenylyl Cyclases