Fatty acid binding to rat liver fatty acid-binding protein is modulated by early glycolytic intermediates.
Fatty acid binding to rat liver fatty acid binding protein in the presence of glycolytic metabolites and at different pH (optimal 7.2) and ionic strength was studied. Binding decreased logarithmically with ionic strength. Glucose and glucose-6-phosphate increased fatty acid binding significantly with K0.5 within physiological ranges while glucose-1-phosphate and phosphate ion caused no effect.
Stewart
J M
JM
Department of Biology and Biochemistry, Mount Allison University, Sackville, New Brunswick, Canada. jstewart@mta.ca
Dewling
V F
VF
Wright
T G
TG
D000818:Animals; D002352:Carrier Proteins / Q000378:metabolism*; D000071438:Fatty Acid-Binding Protein 7; D050556:Fatty Acid-Binding Proteins; D005227:Fatty Acids / Q000378:metabolism*; D005231:Fatty Acids, Unsaturated / Q000378:metabolism; D005456:Fluorescent Dyes / Q000378:metabolism; D005947:Glucose / Q000378:metabolism / Q000494:pharmacology; D019298:Glucose-6-Phosphate / Q000494:pharmacology; D005958:Glucosephosphates / Q000494:pharmacology; D006019:Glycolysis / Q000502:physiology*; D006863:Hydrogen-Ion Concentration; D008099:Liver / Q000378:metabolism*; D018996:Myelin P2 Protein / Q000378:metabolism*; D009363:Neoplasm Proteins; D009419:Nerve Tissue Proteins; D019301:Oleic Acid / Q000378:metabolism; D009994:Osmolar Concentration; D010710:Phosphates / Q000494:pharmacology; D011485:Protein Binding / Q000187:drug effects; D051381:Rats; D011919:Rats, Inbred Strains
D002352:Carrier Proteins; C490174:Fabp7 protein, rat; D000071438:Fatty Acid-Binding Protein 7; D050556:Fatty Acid-Binding Proteins; D005227:Fatty Acids; D005231:Fatty Acids, Unsaturated; D005456:Fluorescent Dyes; D005958:Glucosephosphates; D018996:Myelin P2 Protein; D009363:Neoplasm Proteins; D009419:Nerve Tissue Proteins; D010710:Phosphates; D019301:Oleic Acid; D019298:Glucose-6-Phosphate; C031590:glucose-1-phosphate; D005947:Glucose; C013641:parinaric acid