title: Analysis of a 14-kb fragment containing a putative cell wall gene and a candidate for the ARA1, arabinose kinase, gene from chromosome IV of Arabidopsis thaliana.
abstract: An Arabidopsis thaliana genomic DNA fragment of 14kb has been characterized in the framework of the E.S.S.A. programme. Computational and molecular approaches identified three novel gene sequences coding, respectively, for a protein of unknown function, a putative membrane-anchored cell wall protein and an arabinose kinase gene corresponding to the locus ARA1. The latter two genes named AtSEB1 and AtISA1 have been characterized in detail. They are very different in their organization, codon usage and level of expression. Homologues of AtSEB1 and AtISA1 have been identified. Sequence comparisons showed that the former genes contained a long 5' extension coding for an N-terminal domain probably specifying subcellular localization. Cloning and sequencing of the cognate cDNA for the AtISA1 homologue in A. thaliana, named GAL1, indicate that it encodes for a galactokinase-like protein. Our results highlight the integrative outcome of a systematic sequencing project in which links between biochemically and genetically characterized mutants, ESTs and genomic sequence data are generated.
authors.affiliation: Institut de Biotechnologie des Plantes, Laboratoire de Biologie du Développement des Plantes, Bâtiment 630, Université de Paris-Sud, CNRS-ERS 569, F-91405, Orsay, Cedex, France.
authors.forename: I
authors.initials: I
authors.lastname: Gy
authors.forename: S
authors.initials: S
authors.lastname: Aubourg
authors.forename: S
authors.initials: S
authors.lastname: Sherson
authors.forename: C S
authors.initials: CS
authors.lastname: Cobbett
authors.forename: A
authors.initials: A
authors.lastname: Cheron
authors.forename: M
authors.initials: M
authors.lastname: Kreis
authors.forename: A
authors.initials: A
authors.lastname: Lecharny
mesh_terms: D000595:Amino Acid Sequence; D017360:Arabidopsis / Q000201:enzymology* / Q000235:genetics*; D029681:Arabidopsis Proteins; D002473:Cell Wall / Q000235:genetics; D002874:Chromosome Mapping; D003001:Cloning, Molecular; D003062:Codon; D003360:Cosmids; D017931:DNA Primers; D005091:Exons; D005686:Galactokinase / Q000737:chemistry; D017343:Genes, Plant; D006801:Humans; D008565:Membrane Proteins / Q000096:biosynthesis / Q000737:chemistry / Q000235:genetics; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D017853:Phosphotransferases (Alcohol Group Acceptor) / Q000096:biosynthesis / Q000737:chemistry / Q000235:genetics*; D016133:Polymerase Chain Reaction; D011994:Recombinant Proteins / Q000096:biosynthesis; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid
chemical_list: D029681:Arabidopsis Proteins; D003062:Codon; D017931:DNA Primers; D008565:Membrane Proteins; D011994:Recombinant Proteins; D017853:Phosphotransferases (Alcohol Group Acceptor); C111816:ARA1 protein, Arabidopsis; D005686:Galactokinase