title: MAGE Xp-2: a member of the MAGE gene family isolated from an expression library using systemic lupus erythematosus sera.
abstract: Two regions of the genome contain members of the MAGE gene family; Xq27-qter and Xp21.3. We isolated a transcript, MAGE Xp-2, by screening a cDNA library from the human epithelial carcinoma cell line, HEp-2, using autoantibodies from patients with systemic lupus erythematosus (SLE). The open reading frame (ORF) of MAGE Xp-2 is entirely contained in exon 4, a signature feature of the MAGE gene family. While MAGE Xp-2 shares genomic homology with MAGE Xp-1, the predicted proteins are quite divergent. Specific primers were designed to reliably distinguish between MAGE Xp-1 and MAGE Xp-2 expression. MAGE Xp-2 is expressed in testis, but not in other normal tissues. It is also expressed strongly in two of seven melanoma cell lines and one of four breast carcinomas. MAGE gene expression may be important not only for tumor recognition and cancer therapy, but, because it is the apparent target of autoantibodies in SLE sera, it may also play a role in autoimmune diseases.
authors.affiliation: Department of Pediatric Rheumatology, Children's Hospital of Orange County, California 92868, USA.
authors.forename: D K
authors.initials: DK
authors.lastname: McCurdy
authors.forename: L Q
authors.initials: LQ
authors.lastname: Tai
authors.forename: J
authors.initials: J
authors.lastname: Nguyen
authors.forename: Z
authors.initials: Z
authors.lastname: Wang
authors.forename: H M
authors.initials: HM
authors.lastname: Yang
authors.forename: N
authors.initials: N
authors.lastname: Udar
authors.forename: F
authors.initials: F
authors.lastname: Naiem
authors.forename: P
authors.initials: P
authors.lastname: Concannon
authors.forename: R A
authors.initials: RA
authors.lastname: Gatti
mesh_terms: D000595:Amino Acid Sequence; D000951:Antigens, Neoplasm / Q000737:chemistry / Q000235:genetics; D001323:Autoantibodies / Q000235:genetics / Q000276:immunology; D001483:Base Sequence; D015152:Blotting, Northern; D002874:Chromosome Mapping; D018076:DNA, Complementary; D005091:Exons; D015870:Gene Expression; D015723:Gene Library; D006801:Humans; D007162:Immunoproteins / Q000737:chemistry / Q000235:genetics* / Q000276:immunology; D007438:Introns; D008180:Lupus Erythematosus, Systemic / Q000276:immunology*; D008969:Molecular Sequence Data; D009363:Neoplasm Proteins / Q000737:chemistry; D009369:Neoplasms / Q000235:genetics; D011506:Proteins / Q000737:chemistry* / Q000235:genetics* / Q000276:immunology; D017421:Sequence Analysis; D014407:Tumor Cells, Cultured; D014960:X Chromosome
chemical_list: D000951:Antigens, Neoplasm; D001323:Autoantibodies; D018076:DNA, Complementary; D007162:Immunoproteins; C097488:MAGEB1 protein, human; C072012:MAGEB2 protein, human; D009363:Neoplasm Proteins; D011506:Proteins