Organization of the equine immunoglobulin constant heavy chain genes. I. c epsilon and c alpha genes.
We provide a restriction map of the equine c epsilon and c alpha genes as a molecular basis for isotype classification. Human and murine DNA probes were used for identification of homologous equine DNA sequences and for isolation of the equine c epsilon and c alpha genes from a genomic DNA library. A detailed map of the equine 5'-s epsilon/c epsilon-s alpha/c alpha-3' gene region was obtained. Equine c epsilon and c alpha DNA probes were prepared and used for restriction analysis of immunoglobulin heavy chain gene loci from different horses. This analysis indicated the presence of only one equine c epsilon and one c alpha gene in the haploid equine genome. In addition, for the equine c alpha gene, four haplotypes were identified according to BamHI restriction fragment length polymorphism (RFLP) of genomic DNA. The relative location of the c epsilon and c alpha genes 3' of the equine c mu and c gamma genes was determined by restriction analysis of equi-murine heterohybridomas.
Wagner
B
B
Immunology Unit, Hannover School of Veterinary Medicine, Germany. bwagner@immunologie.tiho-hannover.de
Siebenkotten
G
G
Leibold
W
W
Radbruch
A
A
D000818:Animals; D002874:Chromosome Mapping; D005803:Genes, Immunoglobulin; D006736:Horses / Q000235:genetics* / Q000276:immunology; D006801:Humans; D007127:Immunoglobulin Constant Regions / Q000235:genetics*; D007143:Immunoglobulin Heavy Chains / Q000235:genetics*; D051379:Mice; D011110:Polymorphism, Genetic
D007127:Immunoglobulin Constant Regions; D007143:Immunoglobulin Heavy Chains