title: Towards the isolation of a human malignant extragonadal germ cell tumour-associated breakpoint in chromosome 11q13.
abstract: In a previous study we have defined a subgroup of human malignant extragonadal germ cell tumours that is characterized by complex translocations involving chromosomes 6 and 11 (Echten et al. 1995). Here we report (i) the use of fluorescent in situ hybridization, pulsed field gel electrophoresis and direct visual hybridization techniques to localize the tumour-associated breakpoint within band 11q13, and (ii) the construction of a phage library enriched for this region to facilitate genomic walks towards the breakpoint. Extensive breakpoint-flanking contigs were generated and within these contigs six candidate genes could be identified.
authors.affiliation: Department of Human Genetics, University Hospital Nijmegen, The Netherlands.
authors.forename: R J
authors.initials: RJ
authors.lastname: Sinke
authors.forename: M
authors.initials: M
authors.lastname: van Asseldonk
authors.forename: D
authors.initials: D
authors.lastname: de Bruijn
authors.forename: J A
authors.initials: JA
authors.lastname: Strijk
authors.forename: G
authors.initials: G
authors.lastname: Merkx
authors.forename: D
authors.initials: D
authors.lastname: Olde Weghuis
authors.forename: B
authors.initials: B
authors.lastname: de Jong
authors.forename: J W
authors.initials: JW
authors.lastname: Oosterhuis
authors.forename: A
authors.initials: A
authors.lastname: Geurts van Kessel
mesh_terms: D000328:Adult; D002874:Chromosome Mapping; D002880:Chromosomes, Human, Pair 11; D004273:DNA, Neoplasm; D015723:Gene Library; D015894:Genome, Human; D018237:Germinoma / Q000235:genetics*; D006801:Humans; D017403:In Situ Hybridization; D008297:Male; D013736:Testicular Neoplasms / Q000235:genetics*
chemical_list: D004273:DNA, Neoplasm