PCR-assisted cDNA cloning: a guided tour of the minefield.
Hooft van Huijsduijnen
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Serono Pharmaceutical Research Institute, Geneva, Switzerland.
D051722:ADAM Proteins; D000818:Animals; D003001:Cloning, Molecular / Q000379:methods*; D018076:DNA, Complementary / Q000302:isolation & purification*; D019483:Disintegrins / Q000235:genetics; D005786:Gene Expression Regulation; D006801:Humans; D008565:Membrane Proteins; D008666:Metalloendopeptidases / Q000235:genetics; D009154:Mutation; D009928:Organ Specificity / Q000235:genetics; D010920:Placenta; D016133:Polymerase Chain Reaction / Q000379:methods*; D019914:Taq Polymerase / Q000235:genetics
D018076:DNA, Complementary; D019483:Disintegrins; D008565:Membrane Proteins; D019914:Taq Polymerase; D051722:ADAM Proteins; C110998:ADAM20 protein, human; D008666:Metalloendopeptidases