Coat protein gene sequences of garlic and onion isolates of the onion yellow dwarf potyvirus (OYDV).
Partial genomic sequences from an unknown garlic potyvirus and from an onion isolate of the onion yellow dwarf potyvirus (OYDV) were obtained. Comparison of the deduced amino acid sequences showed a similarity of 88% between the respective viral coat proteins. The garlic potyvirus coat protein was expressed in E. coli cells, purified, and subjected to Western blot analysis using antibodies raised against different garlic-infecting viruses. The expression protein was consistently recognised by anti-OYDV antibodies and did not react with antibodies specific for leek yellow stripe potyvirus (LYSV), garlic common latent carlavirus (GCLV) and shallot latent carlavirus (SLV). Besides, the garlic potyvirus coat protein was obtained as a fusion protein and used as antigen to produce polyclonal antibodies. These antibodies reacted with purified OYDV virions, but failed to recognise LYSV particles. In the light of this evidence the garlic potyvirus was identified as the garlic strain of OYDV.
Kobayashi
K
K
Instituto de Investigaciones en Ingeniería Genética y Biología Molecular, CONICET, Buenos Aires, Argentina.
Rabinowicz
P
P
Bravo-Almonacid
F
F
Helguera
M
M
Conci
V
V
Lot
H
H
Mentaberry
A
A
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D015153:Blotting, Western; D002213:Capsid / Q000235:genetics* / Q000276:immunology / Q000378:metabolism; D003001:Cloning, Molecular; D004926:Escherichia coli / Q000235:genetics; D005737:Garlic / Q000821:virology; D015870:Gene Expression; D008969:Molecular Sequence Data; D019697:Onions / Q000821:virology; D010946:Plants, Medicinal; D017800:Potyvirus / Q000235:genetics* / Q000276:immunology / Q000378:metabolism; D011817:Rabbits; D011993:Recombinant Fusion Proteins / Q000276:immunology; D011995:Recombination, Genetic; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid
D011993:Recombinant Fusion Proteins