[The effect of matrix metalloproteinase inhibitor on pseudomonal proteinases].
We examined the effect of CS-610, a newly developed matrix metalloproteinase (MMP) inhibitor, on pseudomonal proteinase in vitro. Alkaline proteinase (1143-4977 unit/ml Type I collagenase equivalent) and elastase (13.6-22.6 unit/ml Type I collagenase equivalent) were obtained from strains of P. aeruginosa of IID-1117, IID-1030 and IID-1130. Zymographic analysis of cultured broth of P. aeruginosa demonstrated that CS-610 inhibited alkaline proteinase with an IC50 (50% inhibition concentration) of 1.06-29.0 (x 10(-8)M) and elastase with an IC50 of 1.0-33.3 (x 10(-8)M). CS-610 is a potent inhibitor of pseudomonal proteinases.
Kosaku
K
K
Department of Ophthalmology, Nihon University School of Medicine, Tokyo, Japan.
Maeda
N
N
Yokoyama
T
T
Inoue
T
T
Yoshida
Y
Y
Hosokawa
T
T
Sawa
M
M
D004305:Dose-Response Relationship, Drug; D004591:Electrophoresis, Polyacrylamide Gel; D007211:Indoles / Q000494:pharmacology*; D008666:Metalloendopeptidases / Q000037:antagonists & inhibitors*; D011480:Protease Inhibitors / Q000494:pharmacology*; D011550:Pseudomonas aeruginosa / Q000201:enzymology*; D012697:Serine Endopeptidases / Q000302:isolation & purification / Q000378:metabolism*
C112083:CS 610; D007211:Indoles; D011480:Protease Inhibitors; D012697:Serine Endopeptidases; C032923:microbial serine proteinases; D008666:Metalloendopeptidases