title: Characterisation and molecular typing of Burkholderia pseudomallei: are disease presentations of melioidosis clonally related?
abstract: Eighteen cases of culture positive melioidosis caused by Burkholderia pseudomallei, were seen in four geographically separate communities in North Queensland, Australia. The genetic inter-relatedness of the clinical isolates were compared utilising random amplification of polymorphic DNA (RAPD) and multilocus enzyme electrophoresis (MEE). The isolates segregated into two groups that correlated with clinical presentation rather than geographical location. This is the first described association between the varied clinical presentations of this condition and specific molecular type. If proven on larger studies, this may further our understanding of the pathogenesis of this important condition.
authors.affiliation: Townsville General Hospital, Queensland, Australia.
authors.forename: R
authors.initials: R
authors.lastname: Norton
authors.forename: B
authors.initials: B
authors.lastname: Roberts
authors.forename: M
authors.initials: M
authors.lastname: Freeman
authors.forename: M
authors.initials: M
authors.lastname: Wilson
authors.forename: C
authors.initials: C
authors.lastname: Ashhurst-Smith
authors.forename: W
authors.initials: W
authors.lastname: Lock
authors.forename: D
authors.initials: D
authors.lastname: Brookes
authors.forename: J
authors.initials: J
authors.lastname: La Brooy
mesh_terms: D000293:Adolescent; D000328:Adult; D001315:Australia / Q000453:epidemiology; D015373:Bacterial Typing Techniques; D016957:Burkholderia pseudomallei / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D002648:Child; D004586:Electrophoresis; D004798:Enzymes / Q000032:analysis; D005260:Female; D006801:Humans; D008297:Male; D008554:Melioidosis / Q000453:epidemiology / Q000382:microbiology*; D008875:Middle Aged; D017720:Molecular Epidemiology; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D019105:Random Amplified Polymorphic DNA Technique
chemical_list: D004798:Enzymes