Ototoxicity resulting from intracochlear perfusion of Streptococcus pneumoniae in the guinea pig is modified by cefotaxime or amoxycillin pretreatment.
Acute changes in the electrophysiology and ultrastructure of the organ of Corti were studied after microperfusion of c. 5 x 10(6) CFU of serotype 2 Streptococcus pneumoniae D39 or Escherichia coli K-12 directly into the scala tympani of guinea pigs. Hearing loss was assessed by recording the auditory nerve compound action potential response to a 10 kHz tone pip. Mean hearing loss 3 h after pneumococcal perfusion (n = 4) was 44 dB, compared to 6 dB after E. coli perfusion (n = 4) (P<0.001). After pneumococcal perfusion, scanning electron microscopy revealed damage to hair cell stereocilia and cratering of the apical surface of supporting cells. Intraperitoneal injection of 100 mg/kg cefotaxime (n = 4) or 100 mg/kg amoxycillin (n = 4) 30 min before perfusion of pneumococci significantly reduced mean hearing loss to 23 dB (P=0.01) or 20 dB (P=0.01), respectively, and diminished ultrastructural damage. The data suggest that if pneumococci invade the inner ear during meningitis, cochlear deafness may rapidly ensue.
Winter
A J
AJ
Department of Infection, University of Birmingham, UK.
Comis
S D
SD
Osborne
M P
MP
Hayward
T L
TL
Stephen
J
J
Tarlow
M J
MJ
D000658:Amoxicillin / Q000627:therapeutic use*; D000818:Animals; D019072:Antibiotic Prophylaxis; D002439:Cefotaxime / Q000627:therapeutic use*; D002511:Cephalosporins / Q000627:therapeutic use*; D004427:Ear Diseases / Q000150:complications / Q000473:pathology / Q000517:prevention & control; D004594:Electrophysiology; D004926:Escherichia coli / Q000472:pathogenicity; D004927:Escherichia coli Infections / Q000188:drug therapy; D006168:Guinea Pigs; D006198:Hair Cells, Auditory / Q000473:pathology / Q000648:ultrastructure; D006313:Hearing Loss, Central / Q000382:microbiology; D008855:Microscopy, Electron, Scanning; D010406:Penicillins / Q000627:therapeutic use*; D011008:Pneumococcal Infections / Q000188:drug therapy* / Q000517:prevention & control; D012533:Scala Tympani / Q000382:microbiology*; D013296:Streptococcus pneumoniae / Q000472:pathogenicity*
D002511:Cephalosporins; D010406:Penicillins; D000658:Amoxicillin; D002439:Cefotaxime