[Sequencing analysis of human K-ras oncogene exon 1 in Chinese people].
OBJECTIVE
To investigate the mutation of human K-ras oncogene in the blood of patients with lung cancer.


METHODS
PCR and sequencing methods were used to analyse the exon 1. Twelve patients'blood samples and 31 normals' blood samples were detected.


RESULTS
In all 43 samples, the K-ras codon 11 was CCT (proline), and the opposite strand sequenced was AGG. It was different from the standard GCT(Alanine) sequenced by foreign authors.


CONCLUSIONS
The 11th codon of the human K-ras oncogene in Chinese is CCT (Proline). As the K-ras codon 12 is a hot spot, this finding may be of importance to clinical genetic diagnosis.
Gu
Q
Q
Laboratory of Molecular Genetics, The First People's Public Hospital in Huaihua, Hunan 418000 P. R. China.
Li
L
L
Zhang
Y
Y
Li
X
X
Xiao
Y
Y
Wu
X
X
D044466:Asian People / Q000235:genetics*; D002681:China; D003062:Codon; D011905:Genes, ras; D006801:Humans; D008175:Lung Neoplasms / Q000235:genetics; D017421:Sequence Analysis
D003062:Codon