title: A cationic nonselective stretch-activated channel in the Reissner's membrane of the guinea pig cochlea.
abstract: The Reissner's membrane (RM) separates in the mammalian cochlea the K(+)-rich endolymph from the Na(+)-rich perilymph. The patch-clamp technique was used to investigate the transport mechanisms in epithelial cells of RM freshly dissected from the guinea pig cochlea. This study shows a stretch-activated nonselective cationic channel (SA channel) with a linear current-voltage relationship (23 pS) highly selective for cations over anions [K+ approximately Na+ (1) > Ba2+ (0.65) > Ca2+ (0.32) >> Cl- (0.14)] and activated by the intrapipette gradient pressure. The open probability-pressure relationship is best fitted by a Boltzmann distribution (half-maximal pressure = 37.8 mmHg, slope constant = 8.2 mmHg). SA channels exhibit a strong voltage dependency and are insensitive to internal Ca2+, ATP, and fenamates but are blocked by 1 microM GdCl3 in the pipette. They are reversibly activated by in situ superfusion of the cell with hyposmotic solutions. Kinetic studies show that depolarization and mechanical or osmotic stretch modify the closed and open time constants probably by a different mechanism. These channels could participate in pressure-induced modifications of ionic permeability of the RM.
authors.affiliation: Laboratoire d'Otologie Experimentale, Faculté de Médecine Lariboisiére-Saint Louis, Paris, France.
authors.forename: T H
authors.initials: TH
authors.lastname: Yeh
authors.forename: P
authors.initials: P
authors.lastname: Herman
authors.forename: M C
authors.initials: MC
authors.lastname: Tsai
authors.forename: P
authors.initials: P
authors.lastname: Tran Ba Huy
authors.forename: T
authors.initials: T
authors.lastname: Van den Abbeele
mesh_terms: D000818:Animals; D002118:Calcium / Q000494:pharmacology; D002121:Calcium Channel Blockers / Q000494:pharmacology; D003053:Cochlear Duct / Q000187:drug effects / Q000502:physiology*; D004594:Electrophysiology; D004710:Endolymph / Q000502:physiology; D018159:Endolymphatic Hydrops / Q000503:physiopathology; D005439:Flufenamic Acid / Q000494:pharmacology; D005682:Gadolinium / Q000494:pharmacology; D006168:Guinea Pigs; D006706:Homeostasis; D007473:Ion Channels / Q000187:drug effects / Q000502:physiology*; D008297:Male; D009994:Osmolar Concentration; D018408:Patch-Clamp Techniques; D010539:Permeability; D011312:Pressure; D013314:Stress, Mechanical
chemical_list: D002121:Calcium Channel Blockers; D007473:Ion Channels; D005439:Flufenamic Acid; D005682:Gadolinium; D002118:Calcium