title: Depth predictability of stromal pockets in the posterior cornea.
abstract: OBJECTIVE
  To evaluate the predictability of the depth of stromal pockets made in the posterior cornea for the excision of anterior or posterior lamellar corneal buttons with a planned thickness.
  
  
  METHODS
  Stromal corneal pocket dissections were created in human eye bank eyes by making a peripheral arcuate keratotomy incision at 60, 80, or 95% of central pachymetry and creating a pocket from the bottom of the incision across the cornea. Pocket depth was measured by pachymetry immediately after surgery and by light microscopy.
  
  
  RESULTS
  Mean achieved central pocket depth differed by 0.03+/-0.03 mm from the intended depth. Variation in depth across the pocket decreased from 0.07+/-0.02 mm for pockets made at 60% of the intended depth to 0.05+/-0.01 mm for pockets made at 80% depth, and 0.04+/-0.02 mm for pockets made at 95% depth (p < 0.01). Pachymetric and histological measurements of relative pocket depth averaged 64+/-9% and 73+/-7%, respectively, for pockets made at 60% of the intended depth, 82+/-7% and 86+/-3% for pockets made at 80% depth, and 91+/-7% and 92+/-3% for pockets made at 95% depth. The difference between pachymetric and histological relative pocket depth measurements decreased with deeper pocket depth (p < 0.01).
  
  
  CONCLUSIONS
  In the posterior cornea, stromal pockets can be created to within 30 microm from the intended depth. Variation in depth throughout the pocket decreases with deeper pocket depth. Pachymetry is a reliable method to check the achieved pocket depth during surgery; the accuracy of pachymetry readings improves with deeper pocket depth.
authors.affiliation: Cornea Service, Rotterdam Eye Hospital, The Netherlands.
authors.forename: G R
authors.initials: GR
authors.lastname: Melles
authors.forename: G W
authors.initials: GW
authors.lastname: ten Hoope
authors.forename: F J
authors.initials: FJ
authors.lastname: Rietveld
authors.forename: W H
authors.initials: WH
authors.lastname: Beekhuis
authors.forename: P S
authors.initials: PS
authors.lastname: Binder
mesh_terms: D003319:Corneal Stroma / Q000473:pathology / Q000601:surgery*; D006801:Humans; D013508:Ophthalmologic Surgical Procedures; D013536:Suture Techniques