title: Optical monitoring of living brain tissue.
abstract: Optical methods can usefully augment classical techniques such as electrical recording for investigating cellular and network physiology. Here, recent developments in optical methods--including confocal and nonlinear fluorescence imaging, semiconductor array imaging, and improved fluorescent indicators of ion concentration and transmembrane electrical potential--are briefly reviewed, and examples are offered that may suggest potential applications to experimental studies in alcoholism research.
authors.affiliation: Hokkaido University School of Medicine, Sapporo, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Hosokawa
authors.forename: M
authors.initials: M
authors.lastname: Ohta
authors.forename: A
authors.initials: A
authors.lastname: Koyama
authors.forename: K
authors.initials: K
authors.lastname: Saito
authors.forename: A
authors.initials: A
authors.lastname: Fine
mesh_terms: D000437:Alcoholism / Q000473:pathology / Q000503:physiopathology; D000818:Animals; D001921:Brain / Q000187:drug effects* / Q000473:pathology; D001931:Brain Mapping / Q000295:instrumentation*; D046508:Culture Techniques; D000431:Ethanol / Q000494:pharmacology*; D006624:Hippocampus / Q000473:pathology / Q000503:physiopathology; D007091:Image Processing, Computer-Assisted / Q000295:instrumentation*; D008564:Membrane Potentials / Q000187:drug effects / Q000502:physiology; D018613:Microscopy, Confocal / Q000295:instrumentation*; D008856:Microscopy, Fluorescence / Q000295:instrumentation*
chemical_list: D000431:Ethanol