Characterization of Streptomyces sp. strain DRS-1 and its ampicillin transformation product.
Incubation of ampicillin with whole cells of Streptomyces sp. DRS-1 resulted in accumulation of four compounds different from ampicillin. One of them was isolated, purified and partially characterized. On the basis of spectroscopic characteristics, RF value and antibacterial activity the compound was identified as cephalexin. It could also be obtained from ampicillin by using crude protein extract of the strain.
Roy
D
D
Biochemistry Division, Regional Research Laboratory, Jorhat, Assam, India.
Sharma
A
A
Bhowmick
G
G
Roy
M K
MK
Ghosh
A C
AC
D000667:Ampicillin / Q000378:metabolism*; D001426:Bacterial Proteins / Q000378:metabolism; D002457:Cell Extracts; D002506:Cephalexin / Q000737:chemistry / Q000378:metabolism*; D019751:Intramolecular Transferases / Q000378:metabolism; D015394:Molecular Structure; D046915:Penicillin-Binding Proteins; D012988:Soil Microbiology; D013056:Spectrophotometry, Ultraviolet; D013302:Streptomyces / Q000302:isolation & purification / Q000378:metabolism*
D001426:Bacterial Proteins; D002457:Cell Extracts; D046915:Penicillin-Binding Proteins; D000667:Ampicillin; D019751:Intramolecular Transferases; C038371:deacetoxycephalosporin C synthetase; D002506:Cephalexin