Nucleotide and amino acid sequences of the metallo-beta-lactamase, ImiS, from Aeromonas veronii bv. sobria.
The Aeromonas veronii bv. sobria metallo-beta-lactamase gene, imiS, was cloned. The imiS open reading frame extends for 762 bp and encodes a protein of 254 amino acids with a secreted modified protein of 227 amino acids and a predicted pI of 8.1. To confirm the predicted sequence, purified ImiS was digested and the resulting peptides were identified, yielding an identical sequence for ImiS, with 98% identity to CphA. Both possessed the putative active-site sequence Asn-Tyr-His-Thr-Asp at positions 88 to 92, which is unique to the Aeromonas metallo-beta-lactamases.
Walsh
T R
TR
Bristol Centre for Antimicrobial Research and Evaluation, Department of Microbiology and Pathology, Medical School, University of Bristol, United Kingdom. T.R.WALSH@BRISTOL.AC.UK
Neville
W A
WA
Haran
M H
MH
Tolson
D
D
Payne
D J
DJ
Bateson
J H
JH
MacGowan
A P
AP
Bennett
P M
PM
D000333:Aeromonas / Q000201:enzymology / Q000235:genetics*; D000595:Amino Acid Sequence; D001426:Bacterial Proteins; D001483:Base Sequence; D005798:Genes, Bacterial / Q000235:genetics*; D008969:Molecular Sequence Data; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid; D001618:beta-Lactamases / Q000235:genetics*
D001426:Bacterial Proteins; C111082:ImiS protein, Aeromonas veronii; D001618:beta-Lactamases