Large quantity production with extreme convenience of human SDF-1alpha and SDF-1beta by a Sendai virus vector.
We describe a robust expression of human stromal cell-derived factor-1alpha (SDF-1alpha) and SDF-1beta, the members of CXC-chemokine family, with a novel vector system based upon Sendai virus, a non-segmented negative strand RNA virus. Recombinant SDF-1alpha and SDF-1beta were detected as a major protein species in culture supernatants, reached as high as 10 microg/ ml. This remarkable enrichment of the products allowed us to use even the crude supernatants as the source for biological and antiviral assays without further concentration nor purification and will thus greatly facilitate to screen their genetically engineered derivatives.
Moriya
C
C
Department of Viral Infection, Institute of Medical Science, University of Tokyo, Japan.
Shioda
T
T
Tashiro
K
K
Nagasawa
T
T
Ikegawa
M
M
Ohnishi
Y
Y
Kato
A
A
Hu
H
H
Xin
X
X
Hasan
M K
MK
Maekawa
M
M
Takebe
Y
Y
Sakai
Y
Y
Honjo
T
T
Nagai
Y
Y
D019380:Anti-HIV Agents / Q000494:pharmacology; D002460:Cell Line; D054377:Chemokine CXCL12; D019743:Chemokines, CXC / Q000235:genetics* / Q000302:isolation & purification / Q000494:pharmacology; D002633:Chemotaxis / Q000187:drug effects; D003001:Cloning, Molecular / Q000379:methods*; D016207:Cytokines / Q000235:genetics* / Q000302:isolation & purification / Q000494:pharmacology; D019204:GTP-Binding Proteins / Q000037:antagonists & inhibitors; D005822:Genetic Vectors; D015497:HIV-1 / Q000187:drug effects; D006801:Humans; D011994:Recombinant Proteins / Q000235:genetics; D018108:Respirovirus / Q000235:genetics*
D019380:Anti-HIV Agents; C515255:CXCL12 protein, human; D054377:Chemokine CXCL12; D019743:Chemokines, CXC; D016207:Cytokines; D011994:Recombinant Proteins; D019204:GTP-Binding Proteins