An archaeal DNA binding protein from thermophilic Sulfolobus acidocaldarius forms different types of complexes with DNA.
We have characterized a DNA binding protein (DBNP-B) from the thermoacidophilic archaeon Sulfolobus acidocaldarius with respect to its interaction with single and double stranded DNA. The protein in solution exists predominantly as dimer as indicated by cross linking studies. Binding of DBNP-B to etheno DNA and poly (dA) resulted in fluorescence enhancement and hyperchromicity respectively. Ethidium bromide intercalated into DNA was completely displaced by DBNP-B. DNase I digestion of dsDNA was increased at subsaturating concentration of the protein and was inhibited at higher concentrations. These results and electron microscopy indicate that the protein forms different types of novel complexes with DNA at different protein to DNA ratios.
Sreenivas
K
K
Department of Biochemistry, School of Life Sciences, University of Hyderabad, India.
Jaya
K S
KS
Suryanarayana
T
T
D019843:Archaeal Proteins / Q000737:chemistry / Q000378:metabolism* / Q000648:ultrastructure; D010584:Bacteriophage phi X 174; D003432:Cross-Linking Reagents; D019641:DNA, Archaeal / Q000378:metabolism* / Q000648:ultrastructure; D004269:DNA, Bacterial / Q000378:metabolism* / Q000648:ultrastructure; D004277:DNA, Single-Stranded / Q000378:metabolism* / Q000648:ultrastructure; D004279:DNA, Viral / Q000378:metabolism / Q000648:ultrastructure; D004268:DNA-Binding Proteins / Q000737:chemistry / Q000378:metabolism* / Q000648:ultrastructure; D003850:Deoxyribonuclease I; D019281:Dimerization; D004996:Ethidium; D005456:Fluorescent Dyes; D005557:Formaldehyde; D008970:Molecular Weight; D011061:Poly A / Q000378:metabolism; D016945:Sulfolobus acidocaldarius / Q000378:metabolism*
D019843:Archaeal Proteins; D003432:Cross-Linking Reagents; D019641:DNA, Archaeal; D004269:DNA, Bacterial; D004277:DNA, Single-Stranded; D004279:DNA, Viral; D004268:DNA-Binding Proteins; D005456:Fluorescent Dyes; D005557:Formaldehyde; D011061:Poly A; C015465:poly(dA); D003850:Deoxyribonuclease I; D004996:Ethidium