title: The expression of an abscisic acid-responsive glycine-rich protein coincides with the level of seed dormancy in Fagus sylvatica.
abstract: By differential screening of a cDNA library constructed from poly (A+) RNA of ABA-treated seeds of Fagus sylvatica L., we have isolated an ABA-responsive clone that is present in dormant seeds and under conditions that maintain dormancy, but it tends to disappear under conditions breaking seed dormancy. A search of the sequence data bases showed that the clone codes for a Glycine-Rich Protein and has sequence similarity to RNA-binding proteins. The clone, which exibits the characteristics of lea-genes, is up-regulated by ABA and down-regulated by GA3. Paclobutrazol abolishes the effect of GA3, which is restored upon addition of GA3. The possible relationship of this Glycine-Rich Protein to seed dormancy in F. sylvatica is discussed.
authors.affiliation: Departamento de Biología Vegetal (Fisiología Vegetal), Facultad de Biología, Universidad de Salamanca, Spain.
authors.forename: C
authors.initials: C
authors.lastname: Nicolás
authors.forename: D
authors.initials: D
authors.lastname: Rodríguez
authors.forename: F
authors.initials: F
authors.lastname: Poulsen
authors.forename: E N
authors.initials: EN
authors.lastname: Eriksen
authors.forename: G
authors.initials: G
authors.lastname: Nicolás
mesh_terms: D000040:Abscisic Acid / Q000494:pharmacology; D000595:Amino Acid Sequence; D001483:Base Sequence; D018076:DNA, Complementary; D018744:DNA, Plant; D018506:Gene Expression Regulation, Plant; D018525:Germination; D005875:Gibberellins / Q000494:pharmacology; D005998:Glycine / Q000737:chemistry / Q000235:genetics*; D008969:Molecular Sequence Data; D010937:Plant Growth Regulators / Q000494:pharmacology; D010940:Plant Proteins / Q000737:chemistry / Q000235:genetics*; D012639:Seeds / Q000254:growth & development; D014197:Trees / Q000254:growth & development
chemical_list: D018076:DNA, Complementary; D018744:DNA, Plant; C111586:GRPF1 protein, Fagus sylvatica; D005875:Gibberellins; D010937:Plant Growth Regulators; D010940:Plant Proteins; C066246:late embryogenesis abundant protein, plant; D000040:Abscisic Acid; C007842:gibberellic acid; D005998:Glycine