Oxygen free radical scavenger properties of dehydroepiandrosterone.
The microsomes from dehydroepiandrosterone (DHEA)-supplemented animals are good hydroxyl radical scavengers, as demonstrated through electron spin resonance and deoxyribose degradation. The ability of DHEA-supplemented microsomes to react with superoxide radical was also demonstrated through the inhibition of nitroblue-tetrazolium reduction determined by superoxide radicals produced in a hypoxanthine-xanthine oxidase system. DHEA-enriched microsomes, obtained from acutely DHEA-treated rats, become resistant to iron-dependent lipid peroxidation triggered by H2O2/FeSO4 and ascorbate/FeSO4. The direct addition of DHEA to microsomes from untreated rats failed to prevent iron-dependent lipid peroxidation, even if the microsomes were preincubated with DHEA for up to 15 min, indicating that in vivo transformation is required before antioxidant action can be exerted.
Tamagno
E
E
Aragno
M
M
Boccuzzi
G
G
Gallo
M
M
Parola
S
S
Fubini
B
B
Poli
G
G
Danni
O
O
D000818:Animals; D003687:Dehydroepiandrosterone / Q000378:metabolism* / Q000494:pharmacology; D016166:Free Radical Scavengers / Q000378:metabolism*; D007501:Iron / Q000494:pharmacology; D008862:Microsomes, Liver / Q000187:drug effects / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar
D016166:Free Radical Scavengers; D003687:Dehydroepiandrosterone; D007501:Iron