Destruxin-A4 chlorohydrin, a novel destruxin from fungus OS-F68576: isolation, structure determination, and biological activity as an inducer of erythropoietin.
In the course of screening for small-molecule modulators of erythropoietin gene expression, five destruxins were isolated from the fungal culture of OS-F68576. The structures were elucidated by extensive 1H and 13C NMR spectroscopy and by hydrolytic modification. One compound (destruxin-A4 chlorohydrin, 1) is a novel destruxin. All these compounds induced erythropoietin gene expression 5-fold at concentration of 0.2-2 microM.
Cai
P
P
MYCOsearch, OSI Pharmaceuticals, Durham, North Carolina 27707, USA.
Smith
D
D
Katz
B
B
Pearce
C
C
Venables
D
D
Houck
D
D
D002460:Cell Line; D047630:Depsipeptides; D015672:Erythroid Precursor Cells / Q000187:drug effects / Q000378:metabolism; D004921:Erythropoietin / Q000378:metabolism*; D005285:Fermentation; D005658:Fungi / Q000737:chemistry*; D015870:Gene Expression / Q000187:drug effects; D006801:Humans; D009682:Magnetic Resonance Spectroscopy; D010456:Peptides, Cyclic / Q000302:isolation & purification* / Q000494:pharmacology; D011994:Recombinant Proteins; D013268:Stimulation, Chemical
D047630:Depsipeptides; D010456:Peptides, Cyclic; D011994:Recombinant Proteins; C111695:destruxin A4 chlorohydrin; D004921:Erythropoietin