title: Application of high-performance thin-layer chromatography for the detection of organophosphorus insecticides in human serum after acute poisoning.
abstract: We developed a rapid and simple method for identifying 25 commonly used organophosphorus insecticides in human serum using high-performance thin-layer chromatography (HPTLC). These organophosphates were separated on plates with three different developing systems within 6-18 min and detected by means of ultraviolet radiation and coloring reactions with 4-(4-nitrobenzyl)pyridine-tetraethylenepentamine reagent (NT reagent) or palladium chloride reagent (PdCl2 reagent). Each organophosphate was accurately identified by means of the R(F) x 100 value and the spot color in three systems. The detection limits of dichlorvos, fenitrothion, malathion, methidathion, parathion and trichlorfon in serum by the liquid-liquid extraction method were 1.1, 0.12, 0.12, 0.05, 0.6 and 0.1 microg/ml, respectively. These sensitivities may be sufficient to detect those organophosphates in patient serum after acute poisoning.
authors.affiliation: Department of Hospital Pharmacy, Faculty of Medicine, Kyushu University, Fukuoka, Japan.
authors.forename: K
authors.initials: K
authors.lastname: Futagami
authors.forename: C
authors.initials: C
authors.lastname: Narazaki
authors.forename: Y
authors.initials: Y
authors.lastname: Kataoka
authors.forename: H
authors.initials: H
authors.lastname: Shuto
authors.forename: R
authors.initials: R
authors.lastname: Oishi
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D002855:Chromatography, Thin Layer; D005029:Ethylenediamines; D006801:Humans; D007202:Indicators and Reagents; D007306:Insecticides / Q000097:blood* / Q000506:poisoning*; D009943:Organophosphorus Compounds; D010165:Palladium; D011725:Pyridines; D012680:Sensitivity and Specificity
chemical_list: D005029:Ethylenediamines; D007202:Indicators and Reagents; D007306:Insecticides; D009943:Organophosphorus Compounds; D011725:Pyridines; C008633:4-(4-nitrobenzyl)pyridine; D010165:Palladium; C008756:palladium chloride; C034269:tetraethylenepentamine