Reduced MK801 binding in neocortical neurons after AAV-mediated transfections with NMDA-R1 antisense cDNA.
Two adeno-associated virus (AAV)-derived plasmids were constructed with portions of the N-methyl-d-aspartic acid-R1 (NMDA-R1) receptor subunit downstream from the AAV p40-(pJDT95dlk-aR1) or cytomegalovirus (CMV) promoter (pTRUF3-aR1) in an antisense orientation. Each plasmid drove expression of antisense NMDA-R1 in primary rat neocortical neuronal cultures 4 days after transfection as detected by reverse transcriptase-polymerase chain reaction (RT-PCR). Transfection with pTRUF3-aR1 (2x4 microgram) but not with pJDT95dlk-aR1 decreased neuronal [3H]MK-801 binding in a dose-dependent manner.
Shafron
D R
DR
Department of Neurosurgery, College of Medicine, University of Florida, Box 100267, JHMHC, Gainesville, FL 32610, USA.
Simpkins
C E
CE
Jebelli
B
B
Day
A L
AL
Meyer
E M
EM
D000818:Animals; D002478:Cells, Cultured; D002540:Cerebral Cortex / Q000166:cytology / Q000378:metabolism*; D003587:Cytomegalovirus / Q000235:genetics*; D016291:Dizocilpine Maleate / Q000378:metabolism*; D005822:Genetic Vectors / Q000235:genetics*; D051379:Mice; D009474:Neurons / Q000378:metabolism*; D016376:Oligonucleotides, Antisense / Q000494:pharmacology*; D010957:Plasmids / Q000235:genetics; D016133:Polymerase Chain Reaction; D051381:Rats; D016194:Receptors, N-Methyl-D-Aspartate / Q000235:genetics* / Q000378:metabolism*; D014162:Transfection
D016376:Oligonucleotides, Antisense; D016194:Receptors, N-Methyl-D-Aspartate; D016291:Dizocilpine Maleate