Bifunctional structure of two adenylyl cyclases from the myxobacterium Stigmatella aurantiaca.
Two adenylyl cyclase genes (cyaA and cyaB) from the myxobacterium Stigmatella aurantiaca were cloned by complementation of Escherichia coli mutants defective in the cya gene. cyaA codes for a protein of 424 amino acid residues (AC1), while cyaB encodes a protein of 352 residues (AC2). Both cyclases are sensitive to adenosine: cAMP production was strongly inhibited in E coli cells and cell extracts expressing these genes. AC1 comprises a hydrophobic domain of six transmembrane helices coupled to a cytoplasmic catalytic domain endowed with adenylyl cyclase activity. A 17 amino acid residue sequence, which is a signature of G-protein coupled receptors, as well as of slime mold Dictyostelium discoideum cyclic AMP receptors, was found in the membrane domain. AC2 displays features also indicating that it is a bifunctional enzyme. The domain located upstream from the catalytic adenylyl cyclase domain shows strong similarity to receiver modules of response regulators of two-component bacterial signaling systems. In vitro mutagenesis of conserved aspartate residues in this domain was shown to interfere with cAMP synthesis.
Coudart-Cavalli
M P
MP
DKFZ, Deutsches Krebs Forschung Zentrum, Heidelberg, Germany.
Sismeiro
O
O
Danchin
A
A
D000241:Adenosine / Q000494:pharmacology; D037361:Adenylate Cyclase Toxin; D000262:Adenylyl Cyclases / Q000737:chemistry* / Q000235:genetics / Q000302:isolation & purification; D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000737:chemistry* / Q000235:genetics / Q000302:isolation & purification; D001483:Base Sequence; D000242:Cyclic AMP / Q000096:biosynthesis; D004252:DNA Mutational Analysis; D004789:Enzyme Activation / Q000187:drug effects; D008969:Molecular Sequence Data; D009231:Myxococcales / Q000201:enzymology* / Q000235:genetics / Q000378:metabolism; D011498:Protein Precursors / Q000737:chemistry* / Q000235:genetics / Q000302:isolation & purification
D037361:Adenylate Cyclase Toxin; D001426:Bacterial Proteins; D011498:Protein Precursors; D000242:Cyclic AMP; D000262:Adenylyl Cyclases; D000241:Adenosine