Experimental infection of chimpanzees with hepatitis G virus and genetic analysis of the virus.
Hepatitis G virus (HGV) was transmitted to 2 chimpanzees by inoculation with human plasma containing approximately 10(8) genome equivalents (GE) of HGV. The infection was characterized by the late appearance (weeks 10 and 11 after inoculation [pi]) of viremia that persisted throughout the 120-week follow-up. Serum HGV titer increased steadily until it plateaued at 10(6)-10(7) GE/mL. However, despite this relatively high titer, neither of the chimpanzees developed hepatitis. The sequence of the viral genome, recovered from each chimpanzee at week 77 pi, differed from that of the inoculum by 5 nt (2 aa) and 27 nt (2 aa). Two more chimpanzees were inoculated with a first-passage plasma pool. The chimpanzee inoculated with approximately 10(6.7) GE of HGV had viremia at week 1 pi. However, the viral titer increased with the same kinetics as observed in the first passage. The second chimpanzee inoculated with approximately 10(4.7) GE of HGV had late appearance (week 7 pi) of viremia.
Bukh
J
J
Hepatitis Viruses Section, Laboratory of Infectious Diseases, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland 20892, USA.
Kim
J P
JP
Govindarajan
S
S
Apgar
C L
CL
Foung
S K
SK
Wages
J
J
Yun
A J
AJ
Shapiro
M
M
Emerson
S U
SU
Purcell
R H
RH
D019943:Amino Acid Substitution; D000818:Animals; D000914:Antibodies, Viral / Q000032:analysis / Q000276:immunology; D003001:Cloning, Molecular; D018067:Flaviviridae / Q000235:genetics* / Q000276:immunology / Q000302:isolation & purification; D006524:Hepatitis, Viral, Animal / Q000097:blood / Q000235:genetics* / Q000821:virology*; D008099:Liver / Q000201:enzymology; D008969:Molecular Sequence Data; D002679:Pan troglodytes; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D012367:RNA, Viral / Q000032:analysis / Q000235:genetics / Q000302:isolation & purification; D017423:Sequence Analysis, RNA; D014759:Viral Envelope Proteins / Q000276:immunology; D014766:Viremia / Q000175:diagnosis
D000914:Antibodies, Viral; D012367:RNA, Viral; D014759:Viral Envelope Proteins; C107619:glycoprotein E2, GB virus C