title: Hybrid Bordetella pertussis-Escherichia coli RNA polymerases: selectivity of promoter activation.
abstract: We constructed hybrid Bordetella pertussis-Escherichia coli RNA polymerases and compared productive interactions between transcription activators and cognate RNA polymerase subunits in an in vitro transcription system. Virulence-associated genes of B. pertussis, in the presence of their activator BvgA, are transcribed by all variants of hybrid RNA polymerases, whereas transcription at the E. coli lac promoter regulated by the cyclic AMP-catabolite gene activator protein has an absolute requirement for the E. coli alpha subunit. This suggests that activator contact sites involve a high degree of selectivity.
authors.affiliation: Unité de Biochimie Cellulaire, Institut Pasteur, Paris, France.
authors.forename: P
authors.initials: P
authors.lastname: Steffen
authors.forename: A
authors.initials: A
authors.lastname: Ullmann
mesh_terms: D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000032:analysis / Q000235:genetics / Q000378:metabolism; D001886:Bordetella pertussis / Q000235:genetics* / Q000378:metabolism*; D003001:Cloning, Molecular; D000242:Cyclic AMP / Q000378:metabolism; D012321:DNA-Directed RNA Polymerases / Q000235:genetics* / Q000378:metabolism*; D004926:Escherichia coli / Q000235:genetics* / Q000378:metabolism*; D029968:Escherichia coli Proteins; D007763:Lac Operon; D008969:Molecular Sequence Data; D011401:Promoter Regions, Genetic; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000378:metabolism; D016415:Sequence Alignment; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism; D014158:Transcription, Genetic; D001618:beta-Lactamases / Q000235:genetics
chemical_list: D001426:Bacterial Proteins; C065601:BvgA protein, Bacteria; D029968:Escherichia coli Proteins; C489314:EvgA protein, E coli; D011993:Recombinant Fusion Proteins; D014157:Transcription Factors; D000242:Cyclic AMP; D012321:DNA-Directed RNA Polymerases; C074522:beta-lactamase TRC-1; D001618:beta-Lactamases