title: Identification of eicosanoids in the red algae, Gracilaria asiatica, using high-performance liquid chromatography and electrospray ionization mass spectrometry.
abstract: Identification of eicosanoids which are metabolites of arachidonic acid in red algae Gracilaria asiatica, one of the popular seaweeds in Japan, was carried out using high-performance liquid chromatography (HPLC) interfaced with electrospray ionization mass spectrometry. Prostaglandin (PG) E2, 15-keto-PGE2, and 8-hydroxyeicosatetraenoic acid (HETE) were detected as major eicosanoids and PGA2, leukotriene B4 as minor ones in G. asiatica. 8- and 12-HETE had the same retention time in HPLC analysis, but using this analytical method, we were able to identify them.
authors.affiliation: Public Health Laboratory of Chiba Prefecture, Japan.
authors.forename: J
authors.initials: J
authors.lastname: Sajiki
authors.forename: H
authors.initials: H
authors.lastname: Kakimi
mesh_terms: D002851:Chromatography, High Pressure Liquid; D015777:Eicosanoids / Q000032:analysis*; D008401:Gas Chromatography-Mass Spectrometry; D013058:Mass Spectrometry; D000461:Rhodophyta / Q000737:chemistry*; D013056:Spectrophotometry, Ultraviolet
chemical_list: D015777:Eicosanoids