title: Characterization of Cyprinus carpio brain nitric oxide synthase.
abstract: Nitric oxide synthase (NOS) activity is found both in soluble and in particulate fractions of the carp brain. The Km values for arginine are 2.8+/-0.5 and 3.3+/-0.4 microM for the soluble and particulate fractions, respectively. K for NG-monomethyl-L-arginine inhibitor are 2.6+/-0.5 and 2.9+/-0.6 microM, and activation energy for the breakdown of the substrate-enzyme complex 8120+/-710 and 4620+/-450 cal per mole. Carp enzyme shows higher affinity than rat NOS for Ca2+ and for the competitive inhibitor 7-nitroindazole.
authors.affiliation: Department of Animal Biology, University of Modena, Italy.
authors.forename: A
authors.initials: A
authors.lastname: Conte
authors.forename: E
authors.initials: E
authors.lastname: Ottaviani
mesh_terms: D000818:Animals; D001120:Arginine / Q000032:analysis; D001921:Brain / Q000201:enzymology*; D001923:Brain Chemistry / Q000502:physiology; D002347:Carps / Q000378:metabolism*; D002956:Citrulline / Q000096:biosynthesis; D004791:Enzyme Inhibitors / Q000494:pharmacology; D005609:Free Radicals / Q000378:metabolism; D007191:Indazoles / Q000494:pharmacology; D008297:Male; D019001:Nitric Oxide Synthase / Q000037:antagonists & inhibitors / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar; D013045:Species Specificity; D014316:Tritium
chemical_list: D004791:Enzyme Inhibitors; D005609:Free Radicals; D007191:Indazoles; D014316:Tritium; D002956:Citrulline; D001120:Arginine; D019001:Nitric Oxide Synthase; C080122:7-nitroindazole