Continuous flow immunosensor for atrazine detection.
The hapten atrazine was detected under continuous flow conditions using a micro-column which contained immobilized monoclonal antibodies (Ab) against atrazine and atrazine labeled with alkaline phosphatase (An*). The equilibrium of the antibody-hapten system, was achieved by a continuous flow of the tracer An* through the micro-column containing the immobilized antibodies. The activity of the tracer was monitored continuously, after the micro-column, by an amperometric detector using p-hydroquinone phosphate as substrate. When pulses of unlabeled atrazine (An) were added to the An* flowing continuously through the micro-column, An* bound to the antibody was displaced, with a consequent change of the detector signal. By this method atrazine concentrations in the range 9-180 micrograms/l were monitored under conditions of continuous operation. Since the equilibrium condition for the system Ab-An* was continuously restored by the flow of An* through the micro-column the regeneration of the antibody was not required.
Vianello
F
F
Department of Biological Chemistry, University of Padova, Italy.
Signor
L
L
Pizzariello
A
A
Di Paolo
M L
ML
Scarpa
M
M
Hock
B
B
Giersch
T
T
Rigo
A
A
D000818:Animals; D001280:Atrazine / Q000032:analysis*; D015374:Biosensing Techniques; D002417:Cattle; D007118:Immunoassay / Q000379:methods*; D051379:Mice; D012680:Sensitivity and Specificity
D001280:Atrazine