Intramolecular derivatization of 2'-amino-pyrimidine modified RNA with functional groups that is compatible with re-amplification.
To expand the scope of nucleic acid aptamers as a tool for precise molecular recognition, functional groups that are not naturally present in nucleic acid molecules are desired. For in vitro selection these new functional groups must be compatible with the selection process. The present method allows the introduction of succinimide activated side chains at internal amino groups of 2'-amino-pyrimidine-RNA in a combinatorial fashion that is compatible with enzymatic re-amplification.
Kujau
M J
MJ
Hans-Knöll-Institut für Naturstoff-Forschung, Abteilung Zellbiologie und Molekularbiologie, Beutenbergstrasse 11, D-07745 Jena, Germany.
Wölfl
S
S
D001483:Base Sequence; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods; D005452:Fluoresceins; D007202:Indicators and Reagents; D008024:Ligands; D009838:Oligodeoxyribonucleotides / Q000138:chemical synthesis / Q000737:chemistry; D009843:Oligoribonucleotides / Q000138:chemical synthesis / Q000737:chemistry; D011743:Pyrimidines / Q000737:chemistry*; D012313:RNA / Q000737:chemistry* / Q000378:metabolism; D013388:Succinimides; D014158:Transcription, Genetic
D005452:Fluoresceins; D007202:Indicators and Reagents; D008024:Ligands; D009838:Oligodeoxyribonucleotides; D009843:Oligoribonucleotides; D011743:Pyrimidines; D013388:Succinimides; C012180:2-aminopyrimidine; D012313:RNA