Effect of glutamate transporter on neuronal damage induced by photochemical thrombotic brain ischemia.
To study the effects of glutamate transporters on the pathogenesis of brain infarct, pharmacological and histological analyses were carried out on the thrombotic focal ischemic model. Expression of mRNA coding for the glutamate transporter GLAST increased significantly in the penumbra at 72 h following the ischemia. Combined with confocal laser scanning microscopic analysis, double staining showed expression of GLAST mRNA in both neurons and glial cells in the penumbra. L-trans-Pyrrolidine-2,4-dicarboxylate (L-trans-PDC), a glutamate uptake inhibitor, dose-dependently enhanced the volume of the infarct induced by the ischemia. The results suggest that a compensatory increase in the activity of glutamate transporter may accompany pathological changes after ischemic injury.
Yan
Y P
YP
National Key Laboratory of Medical Neurobiology and Department of Neurobiology, Shanghai Medical University, China.
Yin
K J
KJ
Sun
F Y
FY
D018528:ATP-Binding Cassette Transporters / Q000235:genetics / Q000494:pharmacology*; D027322:Amino Acid Transport System X-AG; D000818:Animals; D001692:Biological Transport / Q000502:physiology; D002544:Cerebral Infarction / Q000188:drug therapy; D004305:Dose-Response Relationship, Drug; D005904:Glial Fibrillary Acidic Protein / Q000032:analysis; D002546:Ischemic Attack, Transient / Q000188:drug therapy* / Q000473:pathology; D008297:Male; D009474:Neurons / Q000187:drug effects* / Q000473:pathology; D010777:Photochemistry; D012333:RNA, Messenger / Q000096:biosynthesis; D051381:Rats; D017207:Rats, Sprague-Dawley; D013927:Thrombosis / Q000188:drug therapy* / Q000473:pathology
D018528:ATP-Binding Cassette Transporters; D027322:Amino Acid Transport System X-AG; D005904:Glial Fibrillary Acidic Protein; D012333:RNA, Messenger