Macroschizonts of Theileria annulata as vaccine and diagnostic tools.
T. annulata-infected cells present infection-associated peptides. These peptides represent target molecules of the cytotoxic acting cells. Their preparation and characterization may help to develop a sub-unit vaccine. Our studies show that macroschizont-infected bovine cells can be used as parasite antigen in serology for the detection of parasite-specific antibodies in serum of infected animals. Primers derived from the macroschizont of T. annulata can be used as molecular tools for the detection of parasite DNA in blood samples of carrier cattle.
Ahmed
J S
JS
Forschungszentrum Borstel, FU-Berlin.
Shayan
P
P
Hugel
F U
FU
Biermann
R
R
Ewald
C
C
Schein
E
E
Gerdes
J
J
D000818:Animals; D000913:Antibodies, Protozoan / Q000097:blood; D000953:Antigens, Protozoan / Q000032:analysis / Q000276:immunology; D002353:Carrier State / Q000175:diagnosis / Q000662:veterinary; D002417:Cattle; D002418:Cattle Diseases / Q000175:diagnosis* / Q000276:immunology* / Q000469:parasitology; D008213:Lymphocyte Activation; D016133:Polymerase Chain Reaction; D016052:Protozoan Vaccines; D016796:Theileria annulata / Q000737:chemistry / Q000254:growth & development / Q000276:immunology*; D013801:Theileriasis / Q000175:diagnosis* / Q000276:immunology*
D000913:Antibodies, Protozoan; D000953:Antigens, Protozoan; D016052:Protozoan Vaccines