Peptide dependence of major histocompatibility complex class II specific alloreactive responses.
Splenic cells from transgenic mice, in which a single peptide is complexed to all major histocompatibility complex (MHC) class II molecules, are found to be incapable of triggering primary allogeneic mixed lymphocyte/leucocyte reactions (MLR) when co-cultured with lymphocytes from MHC class II congenic mouse strains. In addition, a single HLA-DR-blocking peptide can completely abrogate the capacity of splenocytes from chimeric HLA-DR/H2-E transgenic mice to stimulate primary MLR of T cells from wild-type mice. These results indicate that the primary alloreactive response is directed against a multitude of peptides presented by allogeneic MHC molecules.
Vidović
D
D
Discovery Research, Hoffmann-La Roche, Inc., Nutley, New Jersey 07110-1199, USA.
Toral
J I
JI
Bolin
D R
DR
Ito
K
K
Nagy
Z A
ZA
D000595:Amino Acid Sequence; D000818:Animals; D005260:Female; D006183:H-2 Antigens / Q000276:immunology; D006684:HLA-DR Antigens / Q000378:metabolism; D000949:Histocompatibility Antigens Class II / Q000276:immunology* / Q000378:metabolism; D007959:Lymphocyte Culture Test, Mixed; D008297:Male; D051379:Mice; D008815:Mice, Inbred Strains; D008822:Mice, Transgenic; D008969:Molecular Sequence Data; D009842:Oligopeptides / Q000276:immunology* / Q000378:metabolism; D013601:T-Lymphocytes / Q000276:immunology
D006183:H-2 Antigens; D006684:HLA-DR Antigens; D000949:Histocompatibility Antigens Class II; D009842:Oligopeptides