Urocortin-like immunoreactivity in the substantia nigra, ventral tegmental area and Edinger-Westphal nucleus of rat.
Neurons showing intense urocortin-like immunoreactivity (Ucn-IR) were found immunohistochemically in the substantia nigra pars compacta (SNc) and ventral tegmental area (VTA), as well as in Edinger-Westphal nucleus (E-W), in the rat brain. Almost all Ucn-immunoreactive neuronal cell bodies in the SNc and VTA showed immunoreactivity for tyrosine hydroxylase. Injection of a retrograde tracer, colloidal gold-labeled WGAapoHRP, into the cervical spinal cord resulted in labeling of some E-W neurons with Ucn-IR. These findings suggest that Ucn is located in the cell bodies of dopaminergic neurons, as well as in the cell bodies of E-W neurons sending axons to the spinal cord.
Yamamoto
H
H
Department of Anatomy, Shiga University of Medical Science, Otsu, Japan.
Maeda
T
T
Fujimura
M
M
Fujimiya
M
M
D000818:Animals; D003346:Corticotropin-Releasing Hormone / Q000032:analysis* / Q000276:immunology; D017979:Gold Colloid; D008297:Male; D009412:Nerve Fibers / Q000737:chemistry / Q000201:enzymology; D009474:Neurons / Q000737:chemistry / Q000201:enzymology / Q000648:ultrastructure; D009802:Oculomotor Nerve / Q000737:chemistry*; D051381:Rats; D017208:Rats, Wistar; D013378:Substantia Nigra / Q000737:chemistry*; D014446:Tyrosine 3-Monooxygenase / Q000032:analysis; D054832:Urocortins; D017557:Ventral Tegmental Area / Q000737:chemistry*; D018998:Wheat Germ Agglutinin-Horseradish Peroxidase Conjugate
D017979:Gold Colloid; D054832:Urocortins; D018998:Wheat Germ Agglutinin-Horseradish Peroxidase Conjugate; D003346:Corticotropin-Releasing Hormone; D014446:Tyrosine 3-Monooxygenase