[Detection of hemophilia A carriers by PCR analysis of hind III polymorphism in the factor VIII gene].
OBJECTIVE
To explore a scheme of using PCR analysis in the detection of carriers of Hind III polymorphism of factor VIII gene of hemophilia A.


METHODS
Implicating intron 19 of the factor VIII gene of 6 patients with the hemophilia A and 207 unrelated X-chromosomes were amplified by PCR and were analysed by means of Amp-RFLPs of Hind III.


RESULTS
The incidence of the polymorphic Hind III sites in the given population was found to be 0.29. The frequence of the Hind III heterozygotes in women calculated according to Hardy-Weinberg equation was 0.41, which proved to be informative enough for carrier detection and prenatal diagnosis of hemophilia A. 2 out of 6 families (33%)examined in this study were informative.


CONCLUSIONS
The new scheme proved to be effective for hemophilia A carrier detection and prenatal diagnosis.
Wu
S
S
Department of Medical Genetics, North China Coal Medical College, Tangshan 063000 P. R. China.
Li
Y
Y
Cui
H
H
Xie
Y
Y
Zheng
J
J
Pan
X
X
Zhang
H
H
Zhao
R
R
Zhang
Q
Q
D015247:Deoxyribonuclease HindIII / Q000235:genetics*; D005169:Factor VIII / Q000235:genetics*; D005260:Female; D006580:Genetic Carrier Screening; D006467:Hemophilia A / Q000235:genetics*; D006801:Humans; D008297:Male; D010323:Passive Cutaneous Anaphylaxis; D011110:Polymorphism, Genetic
D005169:Factor VIII; D015247:Deoxyribonuclease HindIII