Oct3/4-associating proteins from embryonal carcinoma and spermatogenic cells of mouse.
The octamer-binding protein Oct3/4 was postulated to active transcription through protein-protein interactions with hypothetical cellular coactivator(s). We have used a bacterially produced Oct3/4, as a protein-binding probe, to detect by far-Western assay the Oct3/4-associating proteins (OTAPs) from the embryonal carcinoma (EC) cells F9 and pachytene spermatocytes. Both common and cell-specific OTAPs were shown to interact directly with Oct3/4. Differentiation of the EC cells results in disappearance of most of OTAPs, supporting their coactivator nature. Several OTAPs detected in pachytene spermatocyte may represent germ cell-specific Oct3/4 coactivators.
Tomilin
A
A
Institute of Cytology, Russian Academy of Sciences, St. Petersburg, Russia.
Vorob'ev
V
V
Drosdowsky
M
M
Séralini
G E
GE
D000818:Animals; D002457:Cell Extracts; D002467:Cell Nucleus; D004268:DNA-Binding Proteins / Q000235:genetics / Q000378:metabolism*; D054278:Embryonal Carcinoma Stem Cells; D008297:Male; D051379:Mice; D008970:Molecular Weight; D014411:Neoplastic Stem Cells / Q000737:chemistry*; D050814:Octamer Transcription Factor-3; D011485:Protein Binding; D011993:Recombinant Fusion Proteins; D013090:Spermatocytes / Q000737:chemistry*; D013917:Thrombin; D015534:Trans-Activators / Q000032:analysis* / Q000737:chemistry / Q000378:metabolism; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism*
D002457:Cell Extracts; D004268:DNA-Binding Proteins; D050814:Octamer Transcription Factor-3; C494174:Pou5f1 protein, mouse; D011993:Recombinant Fusion Proteins; D015534:Trans-Activators; D014157:Transcription Factors; D013917:Thrombin