Production and characterization of a monoclonal antibody against mannose-sensitive hemagglutinin of Vibrio cholerae.
We have generated murine monoclonal antibodies (MAb) against Vibrio cholerae mannose-sensitive hemagglutinin (MSHA) using conventional hybridoma procedures. Seven hybridomas were obtained and one characterized. Hybridoma 2F12/F1 secreted an antibody of the IgG3 type that reacted with a 17-kDa antigen corresponding to the product of the mshA gene. This MAb inhibited mannose-sensitive agglutination of chicken erythrocytes by EL tor and O139 vibrios. Vibrios expressing MSHA activity inhibited binding of the antibody secreted by 2F12/F1 to MSHA-coated microtiter plates.
Falero
G
G
Departamento de Anticuerpos Monoclonales, Instituto Finlay, La Habana, Cuba.
Rodríguez
B L
BL
Valmaseda
T
T
Pérez
M E
ME
Pérez
J L
JL
Fando
R
R
Robert
A
A
Campos
J
J
Silva
A
A
Sierra
G
G
Benítez
J A
JA
D000818:Animals; D000907:Antibodies, Bacterial / Q000276:immunology*; D000911:Antibodies, Monoclonal; D001426:Bacterial Proteins / Q000276:immunology*; D002645:Chickens; D004797:Enzyme-Linked Immunosorbent Assay; D036781:Fimbriae Proteins; D006386:Hemagglutination Tests; D006388:Hemagglutinins / Q000276:immunology*; D006825:Hybridomas; D015151:Immunoblotting; D008358:Mannose / Q000494:pharmacology; D037601:Mannose-Binding Lectin; D014734:Vibrio cholerae / Q000276:immunology*
D000907:Antibodies, Bacterial; D000911:Antibodies, Monoclonal; D001426:Bacterial Proteins; D006388:Hemagglutinins; D037601:Mannose-Binding Lectin; C101032:MshA protein, Vibrio cholerae; D036781:Fimbriae Proteins; D008358:Mannose