Immunological response of mice to the bovine respiratory syncytial virus fusion glycoprotein expressed in recombinant baculovirus infected insect cells.
Bovine respiratory syncytial virus (BRSV) is a major cause of respiratory disease in calves. The BRSV genome encodes two major glycoproteins, G and F, which are the major targets for the host antibody response. We have expressed the F glycoprotein in insect cells (Sf9) using a recombinant baculovirus vector. A comparison of the F protein expressed in mammalian and insect cells by SDS-PAGE showed that only part of the baculovirus-produced protein was soluble and processed like the native protein. The antigenicity of the soluble form of the F protein expressed in insect cells was identical to that of the F protein expressed in mammalian cells. Immunization with the F protein expressed in insect cells induced neutralizing antibodies in mice. This antigenic preparation adjuvanted with Quil-A produced an increased neutralizing antibody titer and induced protection.
Walravens
K
K
Laboratoire de Microbiologie et d'Immunologie, Facultés Universitaires Notre-Dame de la Paix (F.U.N.D.P.), Namur, Belgium.
Matheise
J P
JP
Knott
I
I
Coppe
P
P
Collard
A
A
Didembourg
C
C
Dessy
F
F
Kettmann
R
R
Letesson
J J
JJ
D000818:Animals; D000914:Antibodies, Viral / Q000276:immunology; D000956:Antigens, Viral / Q000032:analysis / Q000276:immunology; D016367:Baculoviridae / Q000235:genetics; D015153:Blotting, Western; D002478:Cells, Cultured; D002522:Chlorocebus aethiops; D003001:Cloning, Molecular; D015870:Gene Expression; D006023:Glycoproteins / Q000235:genetics / Q000276:immunology; D051379:Mice; D008807:Mice, Inbred BALB C; D009500:Neutralization Tests; D011233:Precipitin Tests; D062605:Quillaja Saponins; D011993:Recombinant Fusion Proteins / Q000276:immunology*; D011995:Recombination, Genetic; D018357:Respiratory Syncytial Virus Infections / Q000276:immunology*; D018112:Respiratory Syncytial Virus, Bovine / Q000235:genetics* / Q000276:immunology*; D012503:Saponins / Q000276:immunology; D014611:Vaccination; D014709:Vero Cells; D014764:Viral Proteins / Q000235:genetics / Q000276:immunology*
D000914:Antibodies, Viral; D000956:Antigens, Viral; D006023:Glycoproteins; D062605:Quillaja Saponins; D011993:Recombinant Fusion Proteins; D012503:Saponins; D014764:Viral Proteins; C046386:Quil A