Fibroblast growth factor-2 and -9 regulate proliferation and production of matrix metalloproteinases in human gliomas.
Fibroblast growth factor (FGF) signaling has been recognized in human gliomas. We tested the effect of FGF-2 and FGF-9 on the proliferation and the expression of matrix metalloproteinases (MMPs) and their inhibitor (TIMP-1) in vitro. Both FGFs showed mitogenic activity on U251MG and NMC-G1 cells. MMP-1 expression and collagenolytic activity of NMC-G1 but not of U251MG, and TIMP-1 expression of both cells were stimulated by FGFs. MMP-2 expression, gelatinolytic activity, and chemoinvasion on the matrigel were not altered. FGFs may regulate proliferation and microenvironmental factors independently in each glioma type.
Miyagi
N
N
Department of Pathology, Kurume University, School of Medicine, Japan.
Kato
S
S
Terasaki
M
M
Shigemori
M
M
Morimatsu
M
M
D001932:Brain Neoplasms / Q000201:enzymology* / Q000473:pathology*; D002455:Cell Division / Q000187:drug effects; D017364:Collagenases / Q000096:biosynthesis; D016222:Fibroblast Growth Factor 2 / Q000494:pharmacology*; D051525:Fibroblast Growth Factor 9; D005346:Fibroblast Growth Factors; D018093:Gelatinases / Q000096:biosynthesis; D015971:Gene Expression Regulation, Enzymologic / Q000187:drug effects; D005910:Glioma / Q000201:enzymology* / Q000473:pathology*; D006133:Growth Substances / Q000494:pharmacology*; D006801:Humans; D020781:Matrix Metalloproteinase 1; D020778:Matrix Metalloproteinase 2; D008666:Metalloendopeptidases / Q000096:biosynthesis*; D009361:Neoplasm Invasiveness; D019715:Tissue Inhibitor of Metalloproteinase-1 / Q000096:biosynthesis; D014407:Tumor Cells, Cultured
C496459:FGF9 protein, human; D051525:Fibroblast Growth Factor 9; D006133:Growth Substances; D019715:Tissue Inhibitor of Metalloproteinase-1; D016222:Fibroblast Growth Factor 2; D005346:Fibroblast Growth Factors; D017364:Collagenases; D018093:Gelatinases; D008666:Metalloendopeptidases; D020778:Matrix Metalloproteinase 2; D020781:Matrix Metalloproteinase 1