title: Nuclear history of a pre-mRNA determines the translational activity of cytoplasmic mRNA.
abstract: The pathways of synthesis and maturation of pre-messenger RNA in the nucleus have a direct effect on the translational efficiency of mRNA in the cytoplasm. The transcription of intron-less mRNA in vivo directs this mRNA towards translational silencing. The presence of an intron at the 5' end of the transcript relieves this silencing, whereas an intron at the 3' end further represses translation. These regulatory events are strongly dependent on the transcription of pre-mRNA in the nucleus. The impact of nuclear history on regulatory events in the cytoplasm provides a novel mechanism for the control of gene expression.
authors.affiliation: Laboratory of Molecular Embryology, National Institute of Child Health and Human Development, NIH, Building 18T, Room 106, Bethesda, MD 20892-5431, USA.
authors.forename: K
authors.initials: K
authors.lastname: Matsumoto
authors.forename: K M
authors.initials: KM
authors.lastname: Wassarman
authors.forename: A P
authors.initials: AP
authors.lastname: Wolffe
mesh_terms: D000818:Animals; D002467:Cell Nucleus / Q000235:genetics*; D015500:Chloramphenicol O-Acetyltransferase / Q000235:genetics; D003593:Cytoplasm / Q000235:genetics*; D006657:Histones / Q000235:genetics; D007438:Introns / Q000235:genetics; D009687:Nuclear Proteins / Q000235:genetics; D009865:Oocytes; D014176:Protein Biosynthesis / Q000235:genetics*; D012313:RNA; D012322:RNA Precursors / Q000096:biosynthesis*; D012326:RNA Splicing / Q000235:genetics; D012333:RNA, Messenger / Q000235:genetics* / Q000378:metabolism; D035561:Transcription Factor TFIIA; D014157:Transcription Factors / Q000235:genetics; D014981:Xenopus
chemical_list: D006657:Histones; D009687:Nuclear Proteins; D012322:RNA Precursors; D012333:RNA, Messenger; C039919:RNA, recombinant; D035561:Transcription Factor TFIIA; D014157:Transcription Factors; C057979:nuclear protein B4, Xenopus laevis; D012313:RNA; D015500:Chloramphenicol O-Acetyltransferase