Effect of topical Na-hyaluronan on hemidesmosome formation in n-heptanol-induced corneal injury.
The present study investigated the effect of Na-hyaluronan (Na-HA) on the hemidesmosome morphogenesis in n-heptanol-induced corneal wounds. Central epithelial wounds were induced in the rabbit cornea by applying a 5.5-mm round filter paper, which was soaked in n-heptanol, for 60 s. 1% Na-HA in phosphate-buffered saline (PBS) or PBS alone were topically administered to the wounded cornea 4 times daily for up to 7 days. Epithelial healing rates during the first 2 days were not altered by Na-HA. The number of hemidesmosomes in the basement membrane of the central cornea was significantly increased in both 3- and 7-day groups after treatment with 1% Na-HA. The results suggest that topically applied 1% Na-HA may enhance the formation of hemidesmosomes during the early healing phase in n-heptanol-induced corneal wounds.
Chung
J H
JH
Department of Ophthalmology, College of Medicine, Ewha Women's University, Seoul, Korea.
Kim
W K
WK
Lee
J S
JS
Pae
Y S
YS
Kim
H J
HJ
D000287:Administration, Topical; D000818:Animals; D002057:Burns, Chemical / Q000378:metabolism*; D002452:Cell Count; D003896:Desmosomes / Q000187:drug effects / Q000378:metabolism*; D019573:Epithelium, Corneal / Q000378:metabolism*; D005126:Eye Burns / Q000139:chemically induced*; D019850:Heptanol; D006820:Hyaluronic Acid / Q000008:administration & dosage / Q000494:pharmacology*; D009024:Morphogenesis; D009883:Ophthalmic Solutions; D011817:Rabbits; D014945:Wound Healing / Q000187:drug effects
D009883:Ophthalmic Solutions; D019850:Heptanol; D006820:Hyaluronic Acid