title: Effect of urocortin and its interaction with adrenocorticotropin (ACTH) secretagogues on ACTH release.
abstract: We examined the effect of urocortin (Ucn) on the adrenocorticotropin (ACTH) release from cultured rat anterior pituitary cells and AtT 20 cells. Synthetic rat (r)Ucn was not soluble in 0.1 N HCl but soluble in alkaline solvents with diminished corticotropin-releasing activity. rUcn dissolved in 0.1 M sodium phosphate buffer as a stock solution maintained its bioactivity and had the equal corticotropin-releasing activity with rat/human corticotropin-releasing factor (r/hCRF). rUcn stimulated the adrenocorticotropin release via CRF-receptors accompanied by the additive effect with r/hCRF, the synergistic effect with arginine vasopressin and the dose-dependent inhibition of a potent CRF-receptor antagonist.
authors.affiliation: Department of Medicine, Hamamatsu University School of Medicine, Japan.
authors.forename: M
authors.initials: M
authors.lastname: Ozawa
authors.forename: Y
authors.initials: Y
authors.lastname: Oki
authors.forename: F
authors.initials: F
authors.lastname: Watanabe
authors.forename: K
authors.initials: K
authors.lastname: Iino
authors.forename: M
authors.initials: M
authors.lastname: Masuzawa
authors.forename: M
authors.initials: M
authors.lastname: Iwabuchi
authors.forename: T
authors.initials: T
authors.lastname: Yoshimi
mesh_terms: D000324:Adrenocorticotropic Hormone / Q000378:metabolism*; D000818:Animals; D001127:Arginine Vasopressin / Q000502:physiology; D002478:Cells, Cultured; D003346:Corticotropin-Releasing Hormone / Q000494:pharmacology* / Q000502:physiology; D008297:Male; D051379:Mice; D010903:Pituitary Gland, Anterior / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley; D018019:Receptors, Corticotropin-Releasing Hormone / Q000037:antagonists & inhibitors; D054832:Urocortins
chemical_list: D018019:Receptors, Corticotropin-Releasing Hormone; D054832:Urocortins; D001127:Arginine Vasopressin; D000324:Adrenocorticotropic Hormone; D003346:Corticotropin-Releasing Hormone