title: Three forms of AU-1 like human rotaviruses differentiated by their overall genomic constellation and by the sequence of their VP8*.
abstract: Insight into the origin of human rotaviruses carrying the AU-1 VP4 allele was gained by examining their genomic RNA constellation using RNA-RNA hybridization and by sequencing the VP8* portion (nucleotides 1-750) of their gene 4. AU-1 like viruses isolated in Israel from children attending outpatient clinics were classified into three sub-genogroups based on RNA-RNA hybridization analysis: Subgenogroup 1 consists of two strains (Ro-5829 and Ro-5960) which belong to the AU-1 genogroup, since all their 11 segments hybridized to AU-1 segments. Subgenogroup 2 consists of one reassortant virus (Ro-5193) of which seven RNA segments hybridized to AU-1 segments and the remaining four segments hybridized to NCDV (bovine rotavirus). Subgenogroup 3 consists of four reassortant viruses (Ro-6460, Ro-6584, Ro-6784 and Ro-7044) which had a common genome constellation: only four of their RNA segments hybridized to AU-1 and the other seven segments hybridized to NCDV segments. Sequence analysis of the VP8* gene also revealed a three level pattern of homology with the AU-1 prototype and the local AU-1 like strains which was consistent with the overall genomic (RNA-RNA) constellation: Subgenogroup 1 had 98-98.1% homology with the AU-1 prototype; Subgenogroup 2 had 96.8% homology with the AU-1 prototype and 95.6-96.7% homology with Subgenogroup 1; Subgenogroup 3 had 95.3-95.6% homology with the prototype AU-1 and 93.4-94.3% homology with Subgenogroup 1. Possible evolutionary pathways are discussed.
authors.affiliation: Central Virology Laboratory, Chaim Sheba Medical Center, Tel-Hashomer, Israel.
authors.forename: R
authors.initials: R
authors.lastname: Gollop
authors.forename: O
authors.initials: O
authors.lastname: Nakagomi
authors.forename: I
authors.initials: I
authors.lastname: Silberstein
authors.forename: L M
authors.initials: LM
authors.lastname: Shulman
authors.forename: H B
authors.initials: HB
authors.lastname: Greenberg
authors.forename: E
authors.initials: E
authors.lastname: Mendelson
authors.forename: I
authors.initials: I
authors.lastname: Shif
mesh_terms: D000483:Alleles; D000595:Amino Acid Sequence; D002213:Capsid / Q000235:genetics*; D036022:Capsid Proteins; D016679:Genome, Viral; D006801:Humans; D008969:Molecular Sequence Data; D010802:Phylogeny; D016601:RNA-Binding Proteins / Q000737:chemistry*; D012401:Rotavirus / Q000145:classification* / Q000235:genetics; D017361:Viral Nonstructural Proteins / Q000737:chemistry*
chemical_list: D036022:Capsid Proteins; D016601:RNA-Binding Proteins; C060339:VP4 protein, Rotavirus; D017361:Viral Nonstructural Proteins; C079234:NS35 protein, rotavirus