Levels of the Vsr endonuclease do not regulate stationary-phase reversion of a Lac- frameshift allele in Escherichia coli.
Vsr endonuclease, which initiates very short patch repair, has been hypothesized to regulate mutation in stationary-phase cells. Overexpression of Vsr does dramatically increase the stationary-phase reversion of a Lac- frameshift allele, but the absence of Vsr has no effect. Thus, at least in this case, Vsr has no regulatory role in stationary-phase mutation, and the effects of Vsr overproduction are likely to be artifactual.
Foster
P L
PL
Department of Environmental Health, Boston University School of Public Health, Massachusetts 02118-2394, USA. pfoster@bu.edu
Rosche
W A
WA
D000483:Alleles; D004260:DNA Repair; D004706:Endodeoxyribonucleases / Q000502:physiology*; D004926:Escherichia coli / Q000235:genetics*; D016368:Frameshift Mutation; D007763:Lac Operon
D004706:Endodeoxyribonucleases; C071421:vsr endonuclease