title: Plasma concentrations of Lp(a) lipoprotein and TGF-beta1 are altered in preeclampsia.
abstract: This study was performed to investigate the possible association between preeclampsia and the plasma concentrations of Lp(a) lipoprotein and TGF-beta1 in a large series of patients. Additionally, correlation between the concentrations of these molecules and the severity of preeclampsia or fetal growth retardation was evaluated. Following clinical examination and biochemical analyses, both electroimmunoassay and RIA technique were used for quantitative determinations of plasma Lp(a) lipoprotein. ELISA technique was used to measure the active form of TGF-beta1 in plasma of pregnant normotensive and preeclamptic women. We examined 154 women with preeclampsia (preeclampsia group) and 76 healthy, pregnant normotensive women (control group). The preeclampsia group was further divided into the following subgroups: mild preeclampsia, severe preeclampsia and preeclampsia with fetal growth retardation. Plasma levels of Lp(a) lipoprotein were lower in the total preeclampsia group as well as in all preeclampsia subgroups (5.45+/-7.41, 5.58+/-8.02, 5.08+/-5.38, and 4.32+/-5.28 mg/dl in the total preeclampsia group, and in subgroups with mild preeclampsia, severe preeclampsia, and preeclampsia with fetal growth retardation, respectively) than in the control group (7.84+/-9.26 mg/dl) as determined by quantitative electroimmunoassay. Corresponding results were obtained with a radioimmunoassay (166.03+/-200.2 U/l in the total preeclampsia group vs. 229.18+/-257.7 U/l in controls). There was good correlation between the two methods used for Lp(a) lipoprotein measurement. The differences between controls and the total preeclampsia group as well as each preeclampsia subgroup were statistically significant by a non-parametric test (one-way Kruskal-Wallis test). Plasma concentrations of the active form of TGF-beta1 were increased in all preeclampsia subgroups as well as in the total group (5.63+/-1.68 ng/ml) compared to controls (4.67+/-1.33 ng/ml). This increase in TGF-beta1 was statistically highly significant. Plasma concentrations of Lp(a) lipoprotein and the active form of TGF-beta1 did not differ significantly between the preeclampsia subgroups. The outcome of this study may suggest involvement of both parameters in the pathophysiology of preeclampsia and may substantiate the notion of a multifactorial etiology of the disease.
authors.affiliation: Institute of Medical Genetics, University of Oslo, and Department of Medical Genetics, Norway. srdjan.djurovic@10ks.uio.no
authors.forename: S
authors.initials: S
authors.lastname: Djurovic
authors.forename: R
authors.initials: R
authors.lastname: Schjetlein
authors.forename: F
authors.initials: F
authors.lastname: Wisløff
authors.forename: G
authors.initials: G
authors.lastname: Haugen
authors.forename: H
authors.initials: H
authors.lastname: Husby
authors.forename: K
authors.initials: K
authors.lastname: Berg
mesh_terms: D000328:Adult; D001161:Arteriosclerosis / Q000503:physiopathology; D001724:Birth Weight; D001794:Blood Pressure / Q000502:physiology; D005260:Female; D005317:Fetal Growth Retardation / Q000503:physiopathology; D005865:Gestational Age; D006801:Humans; D006973:Hypertension / Q000503:physiopathology; D017270:Lipoprotein(a) / Q000097:blood*; D008423:Maternal Age; D011225:Pre-Eclampsia / Q000209:etiology / Q000503:physiopathology*; D011247:Pregnancy; D012307:Risk Factors; D016212:Transforming Growth Factor beta / Q000097:blood*
chemical_list: D017270:Lipoprotein(a); D016212:Transforming Growth Factor beta