Functional expression of basolateral Cl-/HCO3- exchange from rat jejunum in Xenopus laevis oocytes.
Poly(A)+ RNA isolated from rat jejunum was injected into Xenopus laevis oocytes and expression of Cl-/HCO3- antiport was investigated by means of 36Cl- uptake. Two days after injection of 50 ng of poly(A)+ RNA, Cl- uptake was significantly increased with respect to water-injected oocytes. The expressed transport was inhibited by 0.2 mM DIDS, whereas endogenous Cl- uptake was unaffected by this disulphonic stilbene. After sucrose density gradient fractionation, the highest expression of DIDS-sensitive Cl- uptake was detected with mRNA size fraction of about 2-4 kb in length. The expressed Cl- uptake can occur against a Cl- concentration gradient and is unaffected by the known Cl- channel blocker anthracene-9-carboxylic acid. Cl- transport mechanism has properties similar to jejunal basolateral. Cl-/HCO3- exchange with regard to Na+ dependence.
Tosco
M
M
Dipartimento di Fisiologia e Biochimica Generali, Università di Milano, Italy.
Orsenigo
M N
MN
Gastaldi
G
G
Faelli
A
A
D000818:Animals; D017920:Antiporters / Q000096:biosynthesis / Q000235:genetics / Q000378:metabolism*; D001639:Bicarbonates / Q000378:metabolism; D018118:Chloride Channels / Q000378:metabolism; D027963:Chloride-Bicarbonate Antiporters; D002712:Chlorides / Q000378:metabolism; D003001:Cloning, Molecular; D005260:Female; D007583:Jejunum / Q000378:metabolism*; D008297:Male; D009865:Oocytes; D011061:Poly A / Q000378:metabolism; D012335:RNA, Ribosomal / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D012964:Sodium / Q000494:pharmacology; D014982:Xenopus laevis
D017920:Antiporters; D001639:Bicarbonates; D018118:Chloride Channels; D027963:Chloride-Bicarbonate Antiporters; D002712:Chlorides; D012335:RNA, Ribosomal; C044176:poly(A) ribosomal RNA; D011061:Poly A; D012964:Sodium