Novel structural difference between nopaline- and octopine-type trbJ genes: construction of genetic and physical map and sequencing of trb/traI and rep gene clusters of a new Ti plasmid pTi-SAKURA.
We constructed a gene library of a nopaline-type Ti plasmid (called pTi-SAKURA) which was newly isolated from Agrobacterium tumefaciens MAFF301001 of a Japanese cherry tree. The partial sequencing data, which were distributed over the entire plasmid genome, made it possible to assign typical Ti-encoded genes including trb and rep gene clusters. The trb/traI and rep gene clusters were sequenced completely. All the genes in the regions except trbJ were homologous with the corresponding genes on octopine-type Ti plasmids, based on both ORF size and sequence similarity. The trbJ on pTi-SAKURA is similar to that of an octopine-type Ti, but has an extra 282-base segment in its central domain. The above gene organization and sequences suggest a divergence of Ti plasmid during evolution in relation to Rhizobium plasmids, and is discussed in this paper.
Suzuki
K
K
Department of Biological Science, Faculty of Science, Hiroshima University, Higashi-Hiroshima, Japan.
Ohta
N
N
Hattori
Y
Y
Uraji
M
M
Kato
A
A
Yoshida
K
K
D016960:Agrobacterium tumefaciens / Q000235:genetics* / Q000821:virology; D001120:Arginine / Q000031:analogs & derivatives* / Q000235:genetics; D001483:Base Sequence; D002874:Chromosome Mapping; D003001:Cloning, Molecular; D004265:DNA Helicases / Q000235:genetics*; D004268:DNA-Binding Proteins; D029968:Escherichia coli Proteins; D008969:Molecular Sequence Data; D005810:Multigene Family; D010957:Plasmids / Q000235:genetics* / Q000302:isolation & purification; D016133:Polymerase Chain Reaction; D017422:Sequence Analysis, DNA; D012689:Sequence Homology, Nucleic Acid; D015534:Trans-Activators / Q000235:genetics*
D004268:DNA-Binding Proteins; D029968:Escherichia coli Proteins; D015534:Trans-Activators; C045264:replication initiator protein; C008666:nopaline; C005394:octopine; D001120:Arginine; C027093:TraI protein, E coli; D004265:DNA Helicases