title: The signal model: a possible explanation for the conversion of DNA double-strand breaks into chromatid breaks.
abstract: OBJECTIVE
  To present and evaluate the 'signal' model for the formation of radiation-induced chromatid breaks.
  
  
  CONCLUSIONS
  Chromatid breaks in human cells represent the apparent interstitial loss of up to about 40 Mbp of DNA, difficult to account for as single lesions under the classical 'breakage-and-reunion' hypothesis. If breakage-first resulted from two interacting DNA double-strand breaks (dsb) with the loss or displacement of the intervening fragment, a dose-squared relationship would be predicted for chromatid breaks. However, the relationship between chromatid break frequency and dose for human cells is linear. The alternative 'exchange' model of Revell is based on the principle of the interaction of two initiating lesions, thus also predicting a dose-squared relationship for chromatid 'breaks'. The signal model explains the conversion of dsb into chromatid breaks on the assumption that a single dsb generates a signal which triggers the cell to initiate a recombinational exchange involving a large loop of chromatin. Incomplete exchanges would be observed as chromatid breaks. Possible candidates for the signalling molecule(s) are DNA protein kinase (DNA PK) and the ATM protein.
authors.affiliation: School of Biomedical Sciences, University of St Andrews, Scotland, UK.
authors.forename: P E
authors.initials: PE
authors.lastname: Bryant
mesh_terms: D000818:Animals; D016466:CHO Cells; D002842:Chromatids / Q000528:radiation effects*; D006224:Cricetinae; D004249:DNA Damage / Q000528:radiation effects*; D004260:DNA Repair; D004307:Dose-Response Relationship, Radiation; D006801:Humans; D008954:Models, Biological; D012854:Sister Chromatid Exchange; D013997:Time Factors