Dendritic cells cultured from mononuclear cells and CD34 cells in myeloma do not harbour human herpesvirus 8.
Dendritic cells (DC) are antigen-presenting cells with the potential to be a powerful adjuvant in the immunotherapy of haematological malignancy, including myeloma. Recently, human herpesvirus 8 (HHV-8) infection of dendritic cells in the long-term bone marrow stromal cultures of patients with myeloma has been reported. This finding is of great potential importance regarding oncogenesis in myeloma in addition to having significant implications for the use of DC in the immunotherapy of this disease. Therefore DC generated from mobilized blood mononuclear cells (MO-DC) and purified CD34+ cells (CD34-DC) of myeloma patients were examined for the presence of HHV-8 using a sensitive PCR technique. HHV-8 was not demonstrated in MO-DC or CD34-DC and we conclude that these cells remain a suitable vehicle for investigation in the immunotherapy of myeloma.
Cull
G M
GM
Department of Clinical and Laboratory Sciences, University of Nottingham at Nottingham City Hospital.
Timms
J M
JM
Haynes
A P
AP
Russell
N H
NH
Irving
W L
WL
Ball
J K
JK
Thomson
B J
BJ
D018952:Antigens, CD34; D003713:Dendritic Cells / Q000821:virology*; D005434:Flow Cytometry; D006566:Herpesviridae Infections / Q000276:immunology*; D019288:Herpesvirus 8, Human / Q000302:isolation & purification*; D006801:Humans; D009101:Multiple Myeloma / Q000821:virology*; D016133:Polymerase Chain Reaction; D012680:Sensitivity and Specificity
D018952:Antigens, CD34