Genetic alterations in acinic cell carcinoma of the parotid gland determined by microsatellite analysis.
We investigated, for the first time, the genetic alterations at certain chromosomal loci in 25 primary parotid acinic cell carcinomas to define the most frequently altered chromosomal regions and their association with pathologic features and DNA content analysis. Our results showed that 21 (84.0%) of the tumors had alteration in at least one of the loci tested. In general, chromosomal regions at chromosomes 4p, 5q, 6p, and 17p were more frequently altered than those on chromosomes 1p and 1q, 4q, 5p, and 6q. Certain markers at 4p15-16, 6p25-qter, and 17p11 regions showed the highest incidence of LOH, suggesting the presence of tumor suppressor genes associated with the oncogenesis of these tumors. LOH was significantly associated only with tumor grade. No apparent correlation between LOH and other clinicopathologic and DNA content characteristics was identified. Our study broadly defined the chromosomal arms and loci that may be targeted for further localization of the minimally deleted regions involved in the tumorigenesis of these tumors.
el-Naggar
A K
AK
Department of Pathology, University of Texas, M. D. Anderson Cancer Center, Houston 77030, USA.
Abdul-Karim
F W
FW
Hurr
K
K
Callender
D
D
Luna
M A
MA
Batsakis
J G
JG
D000328:Adult; D000368:Aged; D018267:Carcinoma, Acinar Cell / Q000235:genetics* / Q000473:pathology; D002869:Chromosome Aberrations; D005260:Female; D005434:Flow Cytometry; D006801:Humans; D019656:Loss of Heterozygosity; D008297:Male; D008875:Middle Aged; D010307:Parotid Neoplasms / Q000235:genetics* / Q000473:pathology