Presence of salmon gonadotropin-releasing hormone (GnRH) and compounds with GnRH-like activity in the ovary of goldfish.
The presence of ovarian GnRH and/or compounds with GnRH-like activity was investigated in the goldfish ovary. Goldfish ovary was extracted using an acetone/HCl/ether mixture and was purified by Waters C-18 Sep-Pak columns (ovarian extract). The goldfish ovarian extract was found to 1) stimulate gonadotropin release and subunit messenger RNA production in the goldfish pituitary that was inhibited by a GnRH antagonist; 2) stimulate germinal vesicle breakdown in the prophase-I arrested follicle-enclosed goldfish oocytes in vitro, which was inhibited by a GnRH antagonist; 3) immunoreact with various GnRH antisera; and 4) bind to GnRH receptors in the goldfish pituitary and ovary as well as rat pituitary. Further purification with HPLC revealed the presence of two compounds with GnRH-like activity. One with identical chromatographic characteristics, amino acid composition, and primary structure to that of the salmon GnRH (sGnRH), and the other a novel compound with GnRH-like activity.
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Department of Biological Sciences, University of Calgary, Alberta, Canada.
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D000595:Amino Acid Sequence; D000818:Animals; D002851:Chromatography, High Pressure Liquid; D005260:Female; D006054:Goldfish / Q000378:metabolism*; D007987:Gonadotropin-Releasing Hormone / Q000031:analogs & derivatives* / Q000032:analysis* / Q000494:pharmacology; D006065:Gonadotropins, Pituitary / Q000096:biosynthesis / Q000378:metabolism; D008540:Meiosis / Q000187:drug effects; D009865:Oocytes / Q000166:cytology / Q000187:drug effects; D010053:Ovary / Q000737:chemistry*; D010902:Pituitary Gland / Q000187:drug effects / Q000378:metabolism; D011966:Receptors, LHRH / Q000378:metabolism; D017385:Sequence Homology; D014020:Tissue Extracts / Q000302:isolation & purification / Q000494:pharmacology
D006065:Gonadotropins, Pituitary; D011966:Receptors, LHRH; D014020:Tissue Extracts; D007987:Gonadotropin-Releasing Hormone