Developmentally regulated protein synthesis during intraperiplasmic growth of Bdellovibrio bacteriovorus 109J.
Bdellovibrio bacteriovorus 109J is an obligate intraperiplasmic predator of other Gram-negative bacteria. Collision with a suitable prey cell initiates a developmental sequence ultimately resulting in the destruction of the prey cell and the production of progeny bdellovibrios. Two-dimensional gel analysis of patterns of protein synthesis at various times in a synchronously growing culture of Bdellovibrio bacteriovorus 109J revealed over 30 polypeptides whose syntheses are developmentally regulated. The majority of these polypeptides fall into nine categories: attack phase specific or one of eight different kinetic groups expressed during the intraperiplasmic growth phase. The results indicate that Bdellovibrio bacteriovorus 109J has a complex system for regulating gene expression during its developmental cycle.
McCann
M P
MP
Department of Biology, St. Joseph's University, Philadelphia, PA 19131, USA.
Solimeo
H T
HT
Cusick
F
F
Panunti
B
B
McCullen
C
C
D001426:Bacterial Proteins / Q000096:biosynthesis*; D001501:Bdellovibrio / Q000254:growth & development*; D001692:Biological Transport; D002463:Cell Membrane Permeability; D004926:Escherichia coli; D015964:Gene Expression Regulation, Bacterial; D008715:Methionine / Q000378:metabolism; D019897:Periplasm / Q000382:microbiology*
D001426:Bacterial Proteins; D008715:Methionine