Application of high-performance thin-layer chromatography for the detection of organophosphorus insecticides in human serum after acute poisoning.
We developed a rapid and simple method for identifying 25 commonly used organophosphorus insecticides in human serum using high-performance thin-layer chromatography (HPTLC). These organophosphates were separated on plates with three different developing systems within 6-18 min and detected by means of ultraviolet radiation and coloring reactions with 4-(4-nitrobenzyl)pyridine-tetraethylenepentamine reagent (NT reagent) or palladium chloride reagent (PdCl2 reagent). Each organophosphate was accurately identified by means of the R(F) x 100 value and the spot color in three systems. The detection limits of dichlorvos, fenitrothion, malathion, methidathion, parathion and trichlorfon in serum by the liquid-liquid extraction method were 1.1, 0.12, 0.12, 0.05, 0.6 and 0.1 microg/ml, respectively. These sensitivities may be sufficient to detect those organophosphates in patient serum after acute poisoning.
Futagami
K
K
Department of Hospital Pharmacy, Faculty of Medicine, Kyushu University, Fukuoka, Japan.
Narazaki
C
C
Kataoka
Y
Y
Shuto
H
H
Oishi
R
R
D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D002855:Chromatography, Thin Layer; D005029:Ethylenediamines; D006801:Humans; D007202:Indicators and Reagents; D007306:Insecticides / Q000097:blood* / Q000506:poisoning*; D009943:Organophosphorus Compounds; D010165:Palladium; D011725:Pyridines; D012680:Sensitivity and Specificity
D005029:Ethylenediamines; D007202:Indicators and Reagents; D007306:Insecticides; D009943:Organophosphorus Compounds; D011725:Pyridines; C008633:4-(4-nitrobenzyl)pyridine; D010165:Palladium; C008756:palladium chloride; C034269:tetraethylenepentamine