A novel technology for packing and unpacking pilot and production scale columns.
A new method of packing and unpacking large scale chromatography columns is described. This involves use of a 3-position valve that can inject chromatography media into a closed column thereby packing it. This same valve in another state is then used to unpack the column. Heights equivalent to a theoretical plate and asymmetry factors of packs on columns from 280 to 800 mm diameter are discussed.
Hofmann
M
M
Euroflow (UK) Ltd., Unit 4, Stroud, Gloucestershire, UK.
D002853:Chromatography, Liquid / Q000379:methods*; D012685:Sepharose
D012685:Sepharose