Liquid chromatographic determination of beta-naphthoxyacetic acid in tomatoes.
An alternative high-performance liquid chromatographic method for the determination of beta-naphthoxyacetic acid (BNOA) in tomatoes is described. BNOA was extracted from tomatoes with acetone-dichloromethane (2:1). The extract was cleaned up by Bio-Beads S-X3 gel-permeation chromatography and by partitioning. A reversed-phase C18 column was used for HPLC analysis. The mobile phase was acetonitrile-2% acetic acid in water (50:50, v/v) pumped at a flow-rate of 1.0 ml/min. Retention time of BNOA was ca. 7 min with a percentage coefficient of variation of 0.71. Resolution of BNOA was good on the column. Percentage recoveries of BNOA were 79.5 +/- 6.82, 94.8 +/- 2.70 and 86.4 +/- 16.43 for the corresponding spiking levels of 0.5, 1.0 and 2.0 micrograms per g tomato, respectively. Analysis of 10 greenhouse tomato samples from local markets in Ankara showed no BNOA residue.
Gökmen
V
V
Department of Food Engineering, Hacettepe University, Ankara, Turkey.
Acar
J
J
D000096:Acetone; D002849:Chromatography, Gas; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D005504:Food Analysis / Q000379:methods*; D006016:Glycolates / Q000032:analysis*; D018551:Solanum lycopersicum / Q000737:chemistry*; D008752:Methylene Chloride; D010937:Plant Growth Regulators / Q000032:analysis*
D006016:Glycolates; D010937:Plant Growth Regulators; D000096:Acetone; D008752:Methylene Chloride; C008586:2-naphthoxyacetic acid