Uptake and gene expression of naked plasmid DNA in cultured brain microvessel endothelial cells.
Cellular uptake and gene expression of plasmid DNA and its cationic liposome complexes were studied using primary cultures of bovine brain microvessel endothelial cells (BMEC) developed as an in vitro model of the blood-brain barrier. An avid association of naked plasmid DNA with the BMEC monolayer was observed at 37 degreesC, which is comparable to that of the DNA/liposome complex. The cellular association significantly decreased at low temperature (4 degreesC). The binding at 4 degreesC was saturable and significantly inhibited by polyanions involving polyinosinic acid and dextran sulfate, typical ligands for the macrophage scavenger receptors, but not by polycytidylic acid or in the presence of EDTA. Unexpectedly, a significant gene expression in the BMEC was obtained by transfection with naked plasmid DNA although the expression level was lower than that obtained by plasmid DNA/cationic liposome complex. Taken together, cultured capillary endothelial cells derived from the brain are able to take up naked plasmid DNA via a scavenger receptor like-mediated mechanism for polyanions and gene expression in the cells takes place.
Nakamura
M
M
Department of Drug Delivery Research, Graduate School of Pharmaceutical Sciences, Kyoto University, Kyoto, Sakyo-ku, 606-8501, Japan.
Davila-Zavala
P
P
Tokuda
H
H
Takakura
Y
Y
Hashida
M
M
D000818:Animals; D001812:Blood-Brain Barrier / Q000502:physiology; D001921:Brain / Q000378:metabolism*; D002417:Cattle; D002478:Cells, Cultured; D016264:Dextran Sulfate / Q000494:pharmacology; D004730:Endothelium, Vascular / Q000378:metabolism; D015870:Gene Expression / Q000235:genetics*; D008081:Liposomes / Q000378:metabolism; D008565:Membrane Proteins; D008833:Microcirculation / Q000502:physiology; D010714:Phosphatidylethanolamines / Q000494:pharmacology; D010957:Plasmids / Q000493:pharmacokinetics*; D011069:Poly I / Q000494:pharmacology; D000071228:Polyelectrolytes; D011108:Polymers / Q000494:pharmacology; D011485:Protein Binding / Q000502:physiology; D011971:Receptors, Immunologic / Q000378:metabolism; D018110:Receptors, Lipoprotein; D051116:Receptors, Scavenger; D051122:Scavenger Receptors, Class B; D013696:Temperature; D014162:Transfection / Q000235:genetics
D016264:Dextran Sulfate; D008081:Liposomes; D008565:Membrane Proteins; D010714:Phosphatidylethanolamines; D011069:Poly I; D000071228:Polyelectrolytes; D011108:Polymers; D011971:Receptors, Immunologic; D018110:Receptors, Lipoprotein; D051116:Receptors, Scavenger; D051122:Scavenger Receptors, Class B; C454739:Scarb1 protein, mouse; C020888:1,2-dielaidoylphosphatidylethanolamine