title: Multiple developmental requirements of noisette, the Drosophila homolog of the U2 snRNP-associated polypeptide SP3a60.
abstract: We report the cloning of the noisette gene (noi), which encodes the Drosophila melanogaster ortholog of a U2 snRNP-associated splicing factor, SF3a60 (SAP61) in humans and PRP9p in Saccharomyces cerevisiae. Antibodies raised against human SF3a60 recognized NOI in flies, showing a nuclear localization in all the stages examined, including the embryo, the dividing cells of imaginal discs, and the larval polyploid nuclei. NOI is expressed in somatic and germinal cells of both male and female gonads. By mobilization of P transposons, we have generated a large number of noi mutations. Complete loss of function resulted in lethality at the end of embryogenesis, without obvious morphological defects. Hypomorphic alleles revealed multiple roles of noi for the survival and differentiation of male germ cells, the differentiation of female germ cells, and the development of several adult structures.
authors.affiliation: Department of Zoology and Animal Biology, University of Geneva, Switzerland.
authors.forename: V
authors.initials: V
authors.lastname: Meyer
authors.forename: B
authors.initials: B
authors.lastname: Oliver
authors.forename: D
authors.initials: D
authors.lastname: Pauli
mesh_terms: D000483:Alleles; D000818:Animals; D002874:Chromosome Mapping; D003001:Cloning, Molecular; D004251:DNA Transposable Elements; D004268:DNA-Binding Proteins / Q000235:genetics* / Q000378:metabolism; D004331:Drosophila melanogaster / Q000235:genetics* / Q000254:growth & development*; D005260:Female; D006801:Humans; D008297:Male; D009154:Mutation; D051762:PAX2 Transcription Factor; D016601:RNA-Binding Proteins / Q000235:genetics* / Q000378:metabolism; D017413:Ribonucleoprotein, U2 Small Nuclear / Q000378:metabolism*; D019849:Sex Determination Processes; D014157:Transcription Factors / Q000235:genetics* / Q000378:metabolism; D016335:Zinc Fingers
chemical_list: D004251:DNA Transposable Elements; D004268:DNA-Binding Proteins; D051762:PAX2 Transcription Factor; C497318:PAX2 protein, human; D016601:RNA-Binding Proteins; D017413:Ribonucleoprotein, U2 Small Nuclear; D014157:Transcription Factors; C088049:splicing factor 3a