A genetic linkage map of a cichlid fish, the tilapia (Oreochromis niloticus).
We have constructed a genetic map for a tilapia, Oreochromis niloticus, using DNA markers. The segregation of 62 microsatellite and 112 anonymous fragment length polymorphisms (AFLPs) was studied in 41 haploid embryos derived from a single female. We have identified linkages among 162 (93.1%) of these markers. 95% of the microsatellites and 92% of the AFLPs were linked in the final map. The map spans 704 Kosambi cM in 30 linkage groups covering the 22 chromosomes of this species. Twenty-four of these linkage groups contain at least one microsatellite polymorphism. From the number of markers 15 or fewer cM apart, we estimate a total map length of approximately 1000-1200 cM. High levels of interference are observed, consistent with measurements in other fish species. This map is a starting point for the mapping of single loci and quantitative traits in cichlid fishes.
Kocher
T D
TD
Department of Zoology, University of New Hampshire, Durham 03824, USA. thomas.d.kocher@unh.edu
Lee
W J
WJ
Sobolewska
H
H
Penman
D
D
McAndrew
B
B
D000818:Animals; D002874:Chromosome Mapping; D005260:Female; D008040:Genetic Linkage; D016678:Genome; D005838:Genotype; D010471:Perches / Q000235:genetics*; D017210:Tilapia / Q000196:embryology / Q000235:genetics*