title: Genomic structures and chromosomal location of p91, a novel murine regulatory receptor family.
abstract: Recently, we found a novel murine cell-surface glycoprotein, designated as p91, expressed mainly in myeloid cells such as macrophages and mast cells. The molecule has six immunoglobulin-like extracellular domains, a transmembrane segment, and a cytoplasmic tail containing four immunoreceptor tyrosine-based inhibition motif (ITIM) or ITIM-like sequences, resembling the structural features of human killer-cell inhibitory receptors (KIR). Here we show that p91 comprises a polymorphic gene family, harboring one potent inhibitory-type p91 and at least two other p91 genes. Tyrosine-phosphorylated, but not nonphosphorylated, synthetic peptides matching the third ITIM and the fourth ITIM-like sequences, respectively, found in the cytoplasmic portion of p91A, the sole inhibitory-type p91, were associated with the tyrosine phosphatases, SHP-1 and SHP-2. In addition, the phosphotyrosyl peptide matching the third ITIM sequence also bound the inositol 5-phosphatase, SHIP. These results support the notion that p91A may function as an inhibitory cell-surface molecule against cell activation. The p91 genes were shown to be clustered in the proximal region of mouse chromosome 7, a syntenic position of human chromosome 19 where the genes for the KIR family are found. A human cDNA clone cross-hybridizing to a murine p91 probe was isolated from a human spleen cDNA library, and was found to code for a molecule quite similar to members of the immunoglobulin-like transcript (or ILT) family. The gene was found to be located on human chromosome 19q13.3-13.4. These results establish the existence of a novel set of potent regulatory receptors in mouse and man, similar but different from the KIR family.
authors.affiliation: Department of Biotechnology, Faculty of Engineering Okayama University, Tsushima-Naka.
authors.forename: Y
authors.initials: Y
authors.lastname: Yamashita
authors.forename: D
authors.initials: D
authors.lastname: Fukuta
authors.forename: A
authors.initials: A
authors.lastname: Tsuji
authors.forename: A
authors.initials: A
authors.lastname: Nagabukuro
authors.forename: Y
authors.initials: Y
authors.lastname: Matsuda
authors.forename: Y
authors.initials: Y
authors.lastname: Nishikawa
authors.forename: Y
authors.initials: Y
authors.lastname: Ohyama
authors.forename: H
authors.initials: H
authors.lastname: Ohmori
authors.forename: M
authors.initials: M
authors.lastname: Ono
authors.forename: T
authors.initials: T
authors.lastname: Takai
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D002478:Cells, Cultured; D002874:Chromosome Mapping; D002888:Chromosomes, Human, Pair 19 / Q000235:genetics; D003001:Cloning, Molecular; D003593:Cytoplasm / Q000737:chemistry; D018076:DNA, Complementary / Q000302:isolation & purification; D006801:Humans; D017737:Macrophages, Peritoneal; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D008815:Mice, Inbred Strains; D008969:Molecular Sequence Data; D005810:Multigene Family; D012333:RNA, Messenger / Q000032:analysis; D011971:Receptors, Immunologic / Q000737:chemistry / Q000235:genetics* / Q000302:isolation & purification*; D054340:Receptors, KIR; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid
chemical_list: D018076:DNA, Complementary; C105948:Pira1 protein, mouse; C105949:Pirb protein, mouse; D012333:RNA, Messenger; D011971:Receptors, Immunologic; D054340:Receptors, KIR