[Diagnosis of contagious bovine pleuropneumonia: problems and recent developments].
While it is easy to diagnose contagious bovine pleuropneumonia (CBPP) in an animal in the acute clinical stage, subacute and chronic forms are more difficult to diagnose. Recourse to laboratory tests is essential to confirm any suspicion of CBPP. As standard diagnostic procedures (isolation, culture, biochemical tests, serological tests) are lacking in specificity and sensitivity, improvements are needed. Progress in molecular biology techniques has led to new tests, among which are the polymerase chain reaction (PCR) and the enzyme-linked immunosorbent assay (ELISA) using monoclonal antibodies. Application of these techniques to CBPP offers a number of advantages, and has considerably enhanced the specificity and sensitivity of diagnosis.
Dedieu
L
L
Département d'élevage et de médecine vétérinaire (CIRAD-EMVT), Centre d'application de méthodologies pour le diagnostic des maladies animales (CAMDA) de l'Office international des épizooties (OIE), Montpellier, France.
Bréard
A
A
Le Goff
C
C
Lefèvre
P C
PC
D000818:Animals; D000907:Antibodies, Bacterial / Q000097:blood; D002417:Cattle; D002418:Cattle Diseases / Q000175:diagnosis*; D004269:DNA, Bacterial / Q000032:analysis; D003937:Diagnosis, Differential; D004797:Enzyme-Linked Immunosorbent Assay / Q000662:veterinary; D009176:Mycoplasma mycoides / Q000235:genetics / Q000276:immunology / Q000302:isolation & purification*; D011002:Pleuropneumonia, Contagious / Q000175:diagnosis*; D016133:Polymerase Chain Reaction / Q000662:veterinary
D000907:Antibodies, Bacterial; D004269:DNA, Bacterial
Diagnostic de la péripneumonie contagieuse bovine: problèmes et nouveaux développements.