A fifty percent reduction of platelet surface glycoprotein Ib does not affect platelet adhesion under flow conditions.
Glycoprotein (GP) Ib is an adhesion receptor on the platelet surface that binds to von Willebrand Factor (vWF). vWF becomes attached to collagens and other adhesive proteins that become exposed when the vessel wall is damaged. Several investigators have shown that during cardiopulmonary bypass (CPB) surgery and also during platelet activation in vitro by thrombin or thrombin receptor activating peptide (TRAP) GPIb disappears from the platelet surface. Such a disappearance is presumed to lead to a decreased adhesive capacity. In the present study, we show that a 65% decrease in platelet surface expression of GPIb, due to stimulation of platelets in Orgaran anticoagulated whole blood with 15 micromol/L TRAP, had no effect on platelet adhesion to both collagen type III and the extracellular matrix (ECM) of human umbilical vein endothelial cells under flow conditions in a single-pass perfusion system. In contrast to adhesion, ristocetin-induced platelet agglutination was highly dependent on the presence of GPIb. Immunoelectron microscopic studies showed that GPIb almost immediately returned to the platelet surface once platelets had attached to collagen. In a subsequent series of experiments, we showed that when less than 50% of GPIb was blocked by an inhibitory monoclonal antibody against GPIb (6D1), platelet adhesion under flow conditions remained unaffected.
van Zanten
G H
GH
Department of Hematology, University Hospital Utrecht, Utrecht University, Utrecht, The Netherlands.
Heijnen
H F
HF
Wu
Y
Y
Schut-Hese
K M
KM
Slootweg
P J
PJ
de Groot
P G
PG
Sixma
J J
JJ
Nieuwland
R
R
D001792:Blood Platelets / Q000166:cytology* / Q000502:physiology*; D002448:Cell Adhesion / Q000502:physiology; D003094:Collagen / Q000502:physiology; D004730:Endothelium, Vascular / Q000166:cytology* / Q000502:physiology*; D005109:Extracellular Matrix / Q000502:physiology; D006801:Humans; D008854:Microscopy, Electron; D019038:Platelet Glycoprotein GPIb-IX Complex / Q000096:biosynthesis*
D019038:Platelet Glycoprotein GPIb-IX Complex; D003094:Collagen