Fluorescence quenching of ethidium ion by porphyrin cations and quaternary ammonium surfactants in the presence of DNA.
Fluorescence quenching of free and DNA-bound ethidium bromide (EB) by a number of quaternary ammonium and other compounds was studied. For free EB or bound EB at lower DNA concentration the fluorescence quenching follows the Stern-Volmer equation and at higher DNA concentration follows an exponential model. At least at low quencher concentrations the quenching efficiency varies with DNA or NaCl concentrations and is about 100 times greater for bound than free EB. The quenching pathways may involve energy transfer and conformational loosening or distortion of the DNA helix in addition to possible electron transfer.
Jin
W J
WJ
Wei
Y S
YS
Liu
C S
CS
Shen
G L
GL
Yu
R Q
RQ
D004247:DNA / Q000378:metabolism*; D003902:Detergents / Q000378:metabolism; D004996:Ethidium / Q000737:chemistry*; D005453:Fluorescence; D007364:Intercalating Agents / Q000737:chemistry; D015394:Molecular Structure; D009690:Nucleic Acid Conformation; D011166:Porphyrins / Q000737:chemistry* / Q000378:metabolism; D013053:Spectrophotometry; D013501:Surface-Active Agents / Q000494:pharmacology*
D003902:Detergents; D007364:Intercalating Agents; D011166:Porphyrins; D013501:Surface-Active Agents; D004247:DNA; D004996:Ethidium