title: Determination of T-lymphocyte adhesion to endothelial cells using a novel luminescence marker.
abstract: Here we report the quantification of T cell adhesion to endothelial cells using the luminescence marker BioLite. A new application for this substance was established using a microassay for the detection of TK-1 mouse T-lymphoma cell adhesion to unstimulated or TNF alpha-stimulated eEnd.2 mouse vascular endothelioma cells. Prelabelling of TK-1 with the marker resulted in luminescence values linearly related to the number of adherent T cells. The marker was not toxic for T cells or endothelial cells nor did it interfere with the expression or function of adhesion molecules on T cells (LFA-1, VLA-4) or endothelial cells (ICAM-1, VCAM-1). When compared with established techniques to quantify T cell/endothelial cell adhesion, i.e. microscopical evaluation or isotope prelabelling of T cells, this method was found to be just as reliable and sensitive.
authors.affiliation: Department of Dermatology, University of Freiburg, Germany.
authors.forename: C C
authors.initials: CC
authors.lastname: Termeer
authors.forename: J M
authors.initials: JM
authors.lastname: Weiss
authors.forename: H
authors.initials: H
authors.lastname: Dittmar
authors.forename: W
authors.initials: W
authors.lastname: Vanscheidt
authors.forename: E
authors.initials: E
authors.lastname: Schöpf
authors.forename: J C
authors.initials: JC
authors.lastname: Simon
mesh_terms: D000818:Animals; D002244:Carbon; D002448:Cell Adhesion; D002460:Cell Line; D002465:Cell Movement / Q000276:immunology*; D004730:Endothelium, Vascular / Q000166:cytology* / Q000276:immunology; D018799:Intercellular Adhesion Molecule-1 / Q000276:immunology; D008163:Luminescent Measurements; D051379:Mice; D013601:T-Lymphocytes / Q000166:cytology* / Q000276:immunology; D019010:Vascular Cell Adhesion Molecule-1 / Q000276:immunology
chemical_list: C039468:Biolite; D019010:Vascular Cell Adhesion Molecule-1; D018799:Intercellular Adhesion Molecule-1; D002244:Carbon