PCR detection of human cytomegalovirus DNA in clinical specimens using novel UL37 exon 3 and US3 primers.
The sensitivity and specificity of novel UL37 exon 3 (UL37x3) and US3 immediate-early (IE) gene PCR primers to detect human cytomegalovirus (HCMV) DNA in clinical specimens are comparable to those of HCMV DNA polymerase (UL54) primers. The use of these IE primers increases the diagnostic performance of HCMV PCR.
Lesperance
M M
MM
Center for Virology, Immunology, and Infectious Disease Research, Department of Otolaryngology, Children's National Medical Center, Washington, DC 20010, USA.
Contopoulos-Ioannidis
D G
DG
del Pilar Gutiérrez
M
M
Colberg-Poley
A M
AM
D003587:Cytomegalovirus / Q000235:genetics / Q000302:isolation & purification*; D003586:Cytomegalovirus Infections / Q000821:virology*; D017931:DNA Primers / Q000235:genetics; D004279:DNA, Viral / Q000032:analysis* / Q000235:genetics; D004259:DNA-Directed DNA Polymerase / Q000235:genetics; D006801:Humans; D016133:Polymerase Chain Reaction / Q000379:methods*
D017931:DNA Primers; D004279:DNA, Viral; D004259:DNA-Directed DNA Polymerase