A novel mutant gene involved in T-lymphocyte-specific homing into peripheral lymphoid organs on mouse chromosome 4.
Previously, we have shown a mutant mouse DDD/1 with T-cell-specific homing defect that is regulated by an autosomal recessive gene, plt (paucity of lymph node T cells), and seems to be caused by lymph node (LN) stromal cells. In the present study, immunohistochemical analysis showed unusual distribution of T cells in LN, Peyer's patches (PP), and spleen from plt/plt, probably due to the failure of T cells to migrate from blood into the T-cell zone in LN or PP, or into the spleen white pulp across high endothelial venule or marginal zone, respectively, based on the experiments in which labelled T cells were injected intravenously and detected in the tissues. Analysis of surface L-selectin and CD44 suggested that T cells with memory phenotype, probably from afferent lymphatics, recruit into plt/plt LN. Linkage mapping by simple-sequence length polymorphism of genomic DNA from 190 backcross progenies produced by intercrossing with MSM/Ms, linked plt most closely with D4Mit237, and localized at 24.7 cM from cetromere on chromosome 4. We discuss the possibility that a wild-type gene on plt locus encodes a chemokine inducing T-cell-specific homing into peripheral lymphoid tissues.
Nakano
H
H
Department of Immunology, Toho University School of Medicine, Tokyo, Japan.
Mori
S
S
Yonekawa
H
H
Nariuchi
H
H
Matsuzawa
A
A
Kakiuchi
T
T
D000818:Animals; D002454:Cell Differentiation / Q000235:genetics / Q000276:immunology; D002465:Cell Movement / Q000235:genetics* / Q000276:immunology*; D002874:Chromosome Mapping; D002877:Chromosomes, Human; D008040:Genetic Linkage; D006801:Humans; D008221:Lymphoid Tissue / Q000276:immunology*; D051379:Mice; D009154:Mutation; D011506:Proteins / Q000235:genetics*; D013601:T-Lymphocytes / Q000276:immunology* / Q000473:pathology
D011506:Proteins