title: Evidence for arylamine N-acetyltransferase activity in the Escherichia coli.
abstract: N-Acetyltransferase activities with p-aminobenzoic acid and 2-aminofluorene as substrates were determined in isolates of the bacterium Escherichia coli. The N-acetyltransferase activity was determined by an acetyl CoA recycling assay and high pressure liquid chromatography. The N-acetyltransferase activities from a number of E. coli isolates were found to be 0.67 +/- 0.04 nmole/min/mg protein for 2-aminofluorene, and 0.46 +/- 0.02 nmole/min/mg protein for p-aminobenzoic acid. The apparent Km and Vmax values obtained were 2. 85 +/- 0.65 mM and 7.51 +/- 0.86 nmol/min/mg protein, respectively, for 2-aminofluorene, and 2.35 +/- 0.39 mM and 9.43 +/- 0.78 nmol/min/mg protein, respectively, for p-aminobenzoic acid. The optimal pH value for the enzyme activity was 7.0 for both substrates tested. The optimal temperature for enzyme activity was 37 degrees C for both substrates. The N-acetyltransferase activity was inhibited by iodoacetamide: at 0.25 mM iodoacetamide, activity was reduced 50%, and at 1.0 mM, more than 90%. Among a series of divalent cations and salts, Cu2+ and Zn2+ were demonstrated to be the most potent inhibitors. This report is the first demonstration of acetyl CoA:arylamine N-acetyltransferase activity in E. coli.
authors.affiliation: Department of Medicine, China Medical College, Taichung 400, Taiwan, Republic of China.
authors.forename: F C
authors.initials: FC
authors.lastname: Chang
authors.forename: J G
authors.initials: JG
authors.lastname: Chung
mesh_terms: D001191:Arylamine N-Acetyltransferase / Q000187:drug effects / Q000302:isolation & purification / Q000378:metabolism*; D004791:Enzyme Inhibitors / Q000494:pharmacology; D004926:Escherichia coli / Q000201:enzymology*; D006863:Hydrogen-Ion Concentration; D007460:Iodoacetamide / Q000494:pharmacology; D007700:Kinetics; D012492:Salts / Q000494:pharmacology; D013696:Temperature
chemical_list: D004791:Enzyme Inhibitors; D012492:Salts; D001191:Arylamine N-Acetyltransferase; D007460:Iodoacetamide