title: Enhanced expression of alpha(1,3)-fucosyltransferase genes correlates with E-selectin-mediated adhesion and metastatic potential of human lung adenocarcinoma cells.
abstract: Alpha(1,3)- and alpha(1,4)-fucosylated oligosaccharides such as sialyl-Lewis(x) (sialyl-Le(x)) and sialyl-Lewis(a) (sialyl-Le(a)) have been reported to participate in tumor cell adhesion to activated endothelium. We examined by cytofluorometry the expression of Le(x), sialyl-Le(x), sialyl-Le(x) dimeric, Le(a), and sialyl-Le(a) on the surface of two human lung adenocarcinoma cell lines with different lung colonization potential. High expression levels of all of these antigens were detected in the metastatic HAL-8Luc cells, whereas the closely related nonmetastatic HAL-24Luc cells only expressed the sialyl-Le(a) and sialyl-Le(x) dimeric antigens, both at lower level than in HAL-8Luc cells. Five alpha(1,3)-fucosyltransferases (alpha(1,3)-Fuc-T) controlling the synthesis of these molecules have been identified to date (Fuc-TIII-Fuc-IVII). The expression of these five genes was also higher in the metastatic cells than in the nonmetastatic counterparts as was shown by Northern blot analysis. In vitro adhesion assays showed that only the metastatic cell line adheres significantly to E-selectin-expressing human endothelial cells. Moreover, monoclonal antibody (mAb) blockade of E-selectin completely abolished tumor cell binding. Adhesion inhibition experiments using mAbs against sialylated fucosylated oligosaccharides expressed on tumor cells indicated that these antigens are involved in the binding. Anti-sialyl-Lex(x) mAb (CSLEX-1) inhibited adhesion by 85%; it had the most pronounced inhibitory effect. These findings suggest that the overexpression of alpha(1,3)-Fuc-T genes in the metastatic HAL-8Luc cells, compared with HAL-24Luc cells, results in an enhanced surface display of fucosylated oligosaccharides, which contributes to the adhesive capacity of these cells to the activated endothelium and correlates with their lung colonization potential.
authors.affiliation: Departamento Biologia Cellular, Institut de Recerca Oncológia, Barcelona, Spain.
authors.forename: M
authors.initials: M
authors.lastname: Martín-Satué
authors.forename: R
authors.initials: R
authors.lastname: Marrugat
authors.forename: J A
authors.initials: JA
authors.lastname: Cancelas
authors.forename: J
authors.initials: J
authors.lastname: Blanco
mesh_terms: D000230:Adenocarcinoma / Q000201:enzymology* / Q000473:pathology*; D000818:Animals; D000951:Antigens, Neoplasm / Q000096:biosynthesis; D000954:Antigens, Surface / Q000096:biosynthesis; D015152:Blotting, Northern; D018395:CA-19-9 Antigen; D002448:Cell Adhesion / Q000502:physiology; D019040:E-Selectin / Q000502:physiology*; D004727:Endothelium / Q000502:physiology; D005434:Flow Cytometry; D005647:Fucosyltransferases / Q000096:biosynthesis* / Q000235:genetics; D005732:Gangliosides / Q000096:biosynthesis*; D015870:Gene Expression; D006801:Humans; D016256:Lewis X Antigen / Q000096:biosynthesis*; D008175:Lung Neoplasms / Q000201:enzymology* / Q000473:pathology*; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D009362:Neoplasm Metastasis; D009844:Oligosaccharides / Q000096:biosynthesis*; D000080506:Sialyl Lewis X Antigen; D014407:Tumor Cells, Cultured
chemical_list: D000951:Antigens, Neoplasm; D000954:Antigens, Surface; D018395:CA-19-9 Antigen; D019040:E-Selectin; D005732:Gangliosides; D016256:Lewis X Antigen; D009844:Oligosaccharides; D000080506:Sialyl Lewis X Antigen; C069719:sialosyl dimeric Le(x) antigen; C064564:sialyl Le(a) ganglioside; D005647:Fucosyltransferases; C031692:galactoside 3-fucosyltransferase