A metabolite of the tobacco-specific lung carcinogen 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanone in the urine of hospital workers exposed to environmental tobacco smoke.
We analyzed the urine of nonsmoking hospital workers exposed to environmental tobacco smoke for [4-(methylnitrosamino)-1-(3-pyridyl)but-1-yl]-beta-O-D-glucosiduronic acid (NNAL-Gluc), a metabolite of the tobacco-specific lung carcinogen 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanone. Samples were collected three times on a single day from nine workers. Quantitative analysis was carried out by combined gas chromatography-nitrosamine-selective detection. The identity of NNAL-Gluc was confirmed by combined gas chromatography-tandem mass spectrometry. The results demonstrated the presence of NNAL-Gluc in the urine of the exposed subjects. The mean level of NNAL-Gluc +/- SD was 0.059 +/- 0.028 pmol/ml urine (23 pg/ml urine); range, 0.005-0.11 pmol/ml urine. Levels of NNAL-Gluc per milliliter of urine correlated with those of cotinine (r = 0.51; P = 0.029). These results demonstrate for the first time that NNAL-Gluc, a metabolite of the lung carcinogen 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanone, is present in the urine of nonsmokers exposed to environmental tobacco smoke under field conditions.
Parsons
W D
WD
Ste. Anne's Hospital, Ste-Anne-de-Bellevue, Quebec, Canada.
Carmella
S G
SG
Akerkar
S
S
Bonilla
L E
LE
Hecht
S S
SS
D000328:Adult; D000395:Air Pollutants, Occupational / Q000009:adverse effects*; D004784:Environmental Monitoring; D005260:Female; D008401:Gas Chromatography-Mass Spectrometry; D005965:Glucuronates / Q000493:pharmacokinetics*; D006801:Humans; D008175:Lung Neoplasms / Q000209:etiology* / Q000517:prevention & control; D008297:Male; D008875:Middle Aged; D009602:Nitrosamines / Q000493:pharmacokinetics*; D009784:Occupational Diseases / Q000209:etiology* / Q000517:prevention & control; D010564:Personnel, Hospital; D012016:Reference Values; D012307:Risk Factors; D014028:Tobacco Smoke Pollution / Q000009:adverse effects*
C099564:4-((methylnitrosoamino)-1-(3-pyridyl)but-1-yl)beta-omega-glucosiduronic acid; D000395:Air Pollutants, Occupational; D005965:Glucuronates; D009602:Nitrosamines; D014028:Tobacco Smoke Pollution