Fluorescent-protein biosensors: new tools for drug discovery.
Recent improvements in target discovery and high-throughput screening have increased the pressure at key points along the drug-discovery pipeline. High-content screening was developed to ease the bottlenecks formed at the target-validation and lead-optimization points, and a new generation of reagents that report on specific molecular processes in living cells (fluorescent-protein biosensors) have been important in its development. Creative designs of fluorescent-protein biosensors have emerged and been used to measure the molecular dynamics of macromolecules, metabolites and ions. Recent applications of fluorescent-protein biosensors to biological problems have provided a foundation for their use in biotechnology.
Giuliano
K A
KA
Cellomics Inc., Pittsburgh, PA 15238, USA.
Taylor
D L
DL
D015374:Biosensing Techniques; D001709:Biotechnology / Q000639:trends*; D015195:Drug Design; D005456:Fluorescent Dyes; D006801:Humans; D004364:Pharmaceutical Preparations / Q000302:isolation & purification*
D005456:Fluorescent Dyes; D004364:Pharmaceutical Preparations