Assay for detecting IgE and IgG antibodies against Candida albicans cell-wall mannan.
In the present study, we assayed mannan-specific IgE and IgG antibodies in samples of serum isolated from blood collected from adult patients with bronchial asthma, using a liquid-phase method with a polysaccharide, mannan (Mn), purified from Candida albicans (C. alb), and investigated the relationships of allergenicity among a crude extract of C. alb, purified Mn, and acid protease (AP), The correlations between the titers of anti-Mn A and anti-Mn B IgE and IgG were very strong, and the levels of inhibition of anti-Mn A IgE and IgG reactions by Mn A and Mn B were almost identical. Although no common allergenicity was observed between Mn A and AP because there was no correlation between the titers of anti-Mn A and anti-AP IgE, and no inhibition of the anti-Mn A IgE reaction by AP, both antigens were found to exist in crude C. alb. The level of inhibition of anti-crude C. alb IgG reaction by Mn A or Mn B was about 60%. Approximately 70% inhibition of the anti-Mn A IgE reaction was observed for eight different fungal allergen extracts, but no inhibition was observed for 11 of the other fungal allergen extracts tested. The above results indicate that common antigenicity was observed between Mn A and Mn B in the human IgE and IgG antibody production system, and the cross-allergenicity observed among some fungi was considered to be the result of the common antigenicity of Mn isoforms.
Akiyama
K
K
National Sagamihara Hospital, Kanagawa, Japan.
Shida
T
T
Yasueda
H
H
Saito
A
A
Hasegawa
M
M
Maeda
Y
Y
Takesako
K
K
Yamaguchi
H
H
Kato
H
H
D000485:Allergens / Q000276:immunology; D000908:Antibodies, Fungal / Q000032:analysis* / Q000276:immunology; D000918:Antibody Specificity; D000946:Antigens, Fungal / Q000187:drug effects / Q000276:immunology; D002176:Candida albicans / Q000378:metabolism*; D002473:Cell Wall / Q000378:metabolism*; D005658:Fungi / Q000276:immunology; D006801:Humans; D007073:Immunoglobulin E / Q000276:immunology*; D007074:Immunoglobulin G / Q000276:immunology*; D008351:Mannans / Q000276:immunology*; D011402:Pronase / Q000494:pharmacology
D000485:Allergens; D000908:Antibodies, Fungal; D000946:Antigens, Fungal; D007074:Immunoglobulin G; D008351:Mannans; D007073:Immunoglobulin E; D011402:Pronase