title: An oral glutamine load enhances renal acid secretion and function.
abstract: In a recent study, a small oral glutamine load acutely elevated plasma bicarbonate concentrations in healthy adults (Am J Nutr 1995;61:1058-61). The present study was designed to elucidate the renal mechanism underlying the base-generating response to L-glutamine. Accordingly, vehicle (489 mL diet soda) or vehicle plus 2 g L-glutamine (28 mg/kg body wt) was ingested and the gain in extracellular fluid volume bicarbonate was compared with renal acid elimination as either ammonium excretion or tubular acid secretion (titratable acid plus bicarbonate reabsorption). Vehicle alone, which contained 27 mmol acid, did not increase extracellular fluid volume bicarbonate over the 90-min period. In contrast, L-glutamine increased plasma bicarbonate concentration (from 25.4+/-2 to 27.9+/-1 mmol/L, P < 0.05) and extracellular fluid volume bicarbonate by an estimated 39+/-10 mmol. When added to that required to neutralize the ingested acid, the combined total for new bicarbonate generated gave an estimated 66+/-10 mmol. Surprisingly, ammonium excretion accounted for < 2% of this newly generated bicarbonate. However, acid secreted and excreted as net acid (5.2+/-4.0 mmol/90 min) as well as that coupled to enhanced bicarbonate reabsorption (76+/-20 mmol/90 min) readily accounted for the estimated base gain (81+/-24 compared with 66+/-10 mmol/90 min). Concomitant with enhanced renal acid secretion, the oral glutamine load elicited an increase in glomerular filtration rate. These results rule out a role for L-glutamine as a direct precursor of bicarbonate and instead point to an indirect role in accelerating acid secretion, apparently coupled to increased glomerular filtration rate.
authors.affiliation: Department of Physiology, Louisiana State University Medical Center, Shreveport 71130, USA. TWelbo@LSUMC.edu
authors.forename: T
authors.initials: T
authors.lastname: Welbourne
authors.forename: W
authors.initials: W
authors.lastname: Claville
authors.forename: M
authors.initials: M
authors.lastname: Langford
mesh_terms: D000293:Adolescent; D000328:Adult; D001639:Bicarbonates / Q000097:blood / Q000378:metabolism; D005919:Glomerular Filtration Rate; D005973:Glutamine / Q000008:administration & dosage / Q000494:pharmacology*; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007656:Ketoglutaric Acids / Q000378:metabolism; D007668:Kidney / Q000378:metabolism* / Q000502:physiology; D007700:Kinetics; D019344:Lactic Acid / Q000378:metabolism; D008297:Male; D008875:Middle Aged; D000644:Quaternary Ammonium Compounds / Q000378:metabolism
chemical_list: D001639:Bicarbonates; D007656:Ketoglutaric Acids; D000644:Quaternary Ammonium Compounds; D005973:Glutamine; D019344:Lactic Acid