title: The histologic characteristics of primary and restenotic carotid plaque.
abstract: BACKGROUND
  Although smooth muscle cell proliferation is a prominent feature of restenosis in experimental models, the role of cellular proliferation in the initiation and progression of carotid restenosis is not well documented.
  
  
  METHODS
  Between 1985 and 1995, 35 carotid endarterectomies (CEA) in 34 patients were performed for restenosis. Patient risk factors, cerebrovascular symptoms, and operative findings were recorded. Tissue specimens from 29 of these cases and 14 original specimens from the same patient were examined by light microscopy (H&E, trichrome, elastochrome, and Alcian blue) and immunohistochemistry (alpha actin, CD 68, vWF, and proliferating nuclear cell antigen (PCNA)) in order to determine the morphologic characteristics and cellular proliferative activity of the plaque.
  
  
  RESULTS
  Hemodynamically significant recurrent stenosis occurred in the 29 patients (69% symptomatic) between 2 months and 30 years after their initial CEAs. Eleven of 29 (38%) lesions were removed early (< 3 years). Recurrent lesions were characterized based on their components as neointimal thickening, 24% (7/29), neointimal thickening and atherosclerosis, 55% (16/29), or atherosclerotic, 21% (6/29). Nineteen of 29 (66%) plaques were complicated by mural thrombus or intraplaque hemorrhage. An inflammatory cell infiltrate consisting of macrophages and T lymphocytes was observed adjacent to areas of recurrent atherosclerosis and macrophages in regions of intimal thickening. Although infrequently present (generally 1-3% of cells) PCNA-positive cells were detected in 41% (12 of 29) of recurrent and 14% (2 of 14) of primary plaques. No PCNA-positive cells were detected in the remaining 67% (29 of 43) of specimens. There was no statistical difference in the number of PCNA-positive cells in early recurrent lesions compared to those recurring after 3 years (36% vs 44%). PCNA immunoreactivity when present was most commonly noted in macrophages associated with thrombus or atheroma rather than smooth muscle cells.
  
  
  CONCLUSIONS
  Although evidence of cellular proliferation was observed in 40% of recurrent carotid endarterectomy lesions, the proliferation rate was low (1-3%) and unrelated to the time interval of recurrence. Proliferative activity was most pronounced in macrophages associated with intraplaque hemorrhage or atheroma. The contribution of inflammatory cells to the biologic behavior of restenotic lesions requires further investigation.
authors.affiliation: Department of Surgery, Veterans Affairs Medical Center, Tucson, Arizona, USA.
authors.forename: J M
authors.initials: JM
authors.lastname: Marek
authors.forename: C
authors.initials: C
authors.lastname: Koehler
authors.forename: M L
authors.initials: ML
authors.lastname: Aguirre
authors.forename: A
authors.initials: A
authors.lastname: Westerband
authors.forename: A T
authors.initials: AT
authors.lastname: Gentile
authors.forename: J L
authors.initials: JL
authors.lastname: Mills
authors.forename: G C
authors.initials: GC
authors.lastname: Hunter
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D001161:Arteriosclerosis / Q000473:pathology; D001167:Arteritis / Q000473:pathology; D016893:Carotid Stenosis / Q000276:immunology / Q000473:pathology* / Q000601:surgery*; D002455:Cell Division; D016894:Endarterectomy, Carotid; D005260:Female; D006470:Hemorrhage / Q000473:pathology; D006801:Humans; D008264:Macrophages / Q000473:pathology; D008297:Male; D008875:Middle Aged; D009131:Muscle, Smooth, Vascular / Q000473:pathology; D018809:Proliferating Cell Nuclear Antigen / Q000378:metabolism; D012008:Recurrence; D012307:Risk Factors; D013997:Time Factors
chemical_list: D018809:Proliferating Cell Nuclear Antigen