Adenovirus internalization and infection require dynamin.
The cell receptors that facilitate adenovirus internalization into cells have been identified; however, the infectious pathway of virus entry has not been established. Adenovirus entry and infection were examined in HeLa cells lacking or overexpressing mutant dynamin, a protein that specifically regulates clathrin-mediated endocytosis. Expression of mutant dynamin significantly reduced adenovirus internalization and gene delivery, indicating a functional requirement for this molecule. These findings are consistent with virus entry via the clathrin-coated pit pathway.
Wang
K
K
Department of Immunology, The Scripps Research Institute, La Jolla, California 92037, USA.
Huang
S
S
Kapoor-Munshi
A
A
Nemerow
G
G
D000260:Adenoviruses, Human / Q000378:metabolism*; D034281:Dynamins; D020558:GTP Phosphohydrolases / Q000235:genetics / Q000378:metabolism*; D017930:Genes, Reporter; D049452:Green Fluorescent Proteins; D006367:HeLa Cells; D006801:Humans; D008164:Luminescent Proteins / Q000235:genetics / Q000378:metabolism; D016296:Mutagenesis; D013752:Tetracycline / Q000494:pharmacology
D008164:Luminescent Proteins; D049452:Green Fluorescent Proteins; D020558:GTP Phosphohydrolases; D034281:Dynamins; D013752:Tetracycline