Infectivity and immunogenicity of SIVmac/HIV-1 chimeric viruses (SHIVs) with deletions in two or three genes (vpr, nef and vpx).
Two SHIVs with two or three genes deleted (SHIV-drn and SHIV-dxrn) were constructed. The inoculation of monkeys with SHIV-drn resulted in short-term viremia, but inoculation with SHIV-dxrn did not. At 68 weeks post-inoculation, the monkeys were reinoculated with a 100-fold higher dose of each SHIV, but none showed viremia. Killer cell activities against HIV-1 Env were detected in the SHIV-drn- and SHIV-dxrn-inoculated monkeys. Cross-reactive killer activity against HIV-1 Gag and SIVmac Gag was observed in one monkey. Antibodies were not detected in the SHIV-dxrn-inoculated monkeys, but the SHIV-drn-inoculated monkeys showed an anamnestic antibody reaction. These data indicate that SHIV-drn is infectious to and immuno-inducible in macaques but SHIV-dxrn is not.
Igarashi
T
T
Laboratory of Pathogenic Virus, Institute for Virus Research, Kyoto University, Japan.
Kuwata
T
T
Yamamoto
H
H
Moriyama
H
H
Ui
M
M
Miyazaki
Y
Y
Hayami
M
M
D016915:AIDS Vaccines / Q000276:immunology; D000163:Acquired Immunodeficiency Syndrome / Q000517:prevention & control; D000818:Animals; D000914:Antibodies, Viral / Q000096:biosynthesis; D003429:Cross Reactions; D003602:Cytotoxicity, Immunologic; D017353:Gene Deletion; D015686:Gene Products, env / Q000235:genetics / Q000276:immunology; D015683:Gene Products, gag / Q000235:genetics / Q000276:immunology; D005814:Genes, Viral; D015754:Genes, nef; D016334:Genes, vpr; D015483:HIV Antibodies / Q000096:biosynthesis; D015497:HIV-1 / Q000235:genetics* / Q000276:immunology*; D007156:Immunologic Memory; D008251:Macaca; D016865:Reassortant Viruses / Q000235:genetics* / Q000276:immunology*; D011993:Recombinant Fusion Proteins; D016097:Simian Acquired Immunodeficiency Syndrome / Q000517:prevention & control / Q000821:virology; D015302:Simian Immunodeficiency Virus / Q000235:genetics* / Q000276:immunology* / Q000502:physiology; D014613:Vaccines, Attenuated / Q000276:immunology; D019562:Viral Load; D054334:Viral Regulatory and Accessory Proteins / Q000235:genetics; D014766:Viremia
D016915:AIDS Vaccines; D000914:Antibodies, Viral; D015686:Gene Products, env; D015683:Gene Products, gag; D015483:HIV Antibodies; D011993:Recombinant Fusion Proteins; C074286:VPX protein, Human immunodeficiency virus 2; C513059:VPX protein, Simian immunodeficiency virus; D014613:Vaccines, Attenuated; D054334:Viral Regulatory and Accessory Proteins