Survival of transfused CD18-positive granulocytes and their chemiluminescent response in a heifer with leukocyte adhesion deficiency.
Granulocyte transfusion (GT) was performed in an 8-month-old heifer with leukocyte adhesion deficiency (BLAD) to monitor the changes in transfused CD18-positive neutrophils and associated neutrophil chemiluminescent (CL) response in beta 2-integrin-deficient host. The CD18-positive neutrophils were detected in blood from the BLAD heifer during the first 3 hr after 2.6 x 10(9) cells were infused by GT, and disappeared by 5 hr after GT. The CL response of neutrophils was increased 1.7 to 2.8-fold in the BLAD heifer during the first 3 hr after GT, thereafter CL response decreased gradually from 2 to 5 hr after GT.
Nagahata
H
H
Department of Animal Health, School of Veterinary Medicine, Rakuno Gakuen University, Ebetsu, Hokkaido, Japan.
Sakurai
N
N
Matsuki
S
S
Miura
T
T
Nioka
K
K
Kociba
G J
GJ
D000818:Animals; D015415:Biomarkers; D018821:CD18 Antigens / Q000097:blood*; D002417:Cattle; D002418:Cattle Diseases; D002470:Cell Survival; D005260:Female; D017708:Leukocyte Transfusion / Q000662:veterinary*; D018370:Leukocyte-Adhesion Deficiency Syndrome / Q000628:therapy / Q000662:veterinary*; D008163:Luminescent Measurements; D009504:Neutrophils / Q000166:cytology / Q000276:immunology / Q000502:physiology*
D015415:Biomarkers; D018821:CD18 Antigens