Attempted transmission of Babesia major by Boophilus microplus.
Two experiments was carried out to determine if Babesia major could be transmitted by Boophilus microplus. In experiment 1, a Babesia-free batch of laboratory reared Bo. microplus larvae were applied to an intact calf infected by inoculation with a B. major stabilate. The calf showed a B. major parasitaemia while the larvae, nymphs and adult ticks were engorging. The engorged females were cultured and batches were incubated at one of the three following temperatures: 24, 28 or 32 degrees C. Approximately 10,000 larvae derived from each of the females were used to infest each of three splenectomized calves. In experiment 2, Babesia-free Bo. microplus larvae were applied to a splenectomized calf; the calf was injected with B. major stabilate and showed a B. major parasitaemia during the adult stage of tick development. The engorged females were incubated at room temperature and the resulting larvae (approximately 10,000) were used to infest a splenectomized calf. Examination of blood films for the presence of B. major from the four calves infested by the second generation larvae in the two experiments were negative.
Yin
H
H
Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Gansu, The People's Republic of China.
Lu
W
W
Zhang
Q
Q
Lu
W
W
Luo
J
J
Dou
H
H
D000818:Animals; D001096:Arachnid Vectors / Q000469:parasitology*; D001403:Babesia / Q000502:physiology*; D001404:Babesiosis / Q000635:transmission*; D002417:Cattle; D002418:Cattle Diseases / Q000469:parasitology / Q000635:transmission*; D002681:China; D005260:Female; D006790:Host-Parasite Interactions; D018512:Parasitemia / Q000662:veterinary; D013984:Tick Infestations / Q000469:parasitology / Q000662:veterinary; D013987:Ticks / Q000469:parasitology*