title: A cytopathogenic, apoptosis-inducing variant of hepatitis A virus.
abstract: A cytopathogenic variant of hepatitis A virus (HAV(cyt/HB1.1)) was isolated from persistently infected BS-C-1 cells by serial passages in FRhK-4 cells. This virus shows a rapid replication pattern and high final titers are obtained, which are main characteristics of cytopathogenic HAVs. Sequencing of the nontranslated regions and the coding regions for 2ABC and 3AB revealed that mutations are distributed all over these regions and that certain mutated sites correspond to those in other cytopathogenic HAV variants. Investigating the mechanisms causing the cytopathic effect in FRhK-4 cells infected with this variant, we found that an apoptotic reaction takes place.
authors.affiliation: Department of Virology, University of Bremen, Germany.
authors.forename: K
authors.initials: K
authors.lastname: Brack
authors.forename: W
authors.initials: W
authors.lastname: Frings
authors.forename: A
authors.initials: A
authors.lastname: Dotzauer
authors.forename: A
authors.initials: A
authors.lastname: Vallbracht
mesh_terms: D000818:Animals; D017209:Apoptosis; D002460:Cell Line; D002522:Chlorocebus aethiops; D003588:Cytopathogenic Effect, Viral; D053938:DNA Fragmentation; D014644:Genetic Variation; D016679:Genome, Viral; D006507:Hepatovirus / Q000235:genetics / Q000254:growth & development / Q000378:metabolism / Q000472:pathogenicity*; D006801:Humans; D017382:Reactive Oxygen Species; D014407:Tumor Cells, Cultured
chemical_list: D017382:Reactive Oxygen Species