Multidrug resistance phenotype conferred by overexpressing bfr2+/pad1+/sks1+ or pap1+ genes and mediated by bfr1+ gene product, a structural and functional homologue of P-glycoprotein in Schizosaccharomyces pombe.
We investigated the mechanism of multidrug resistance conferred by overexpression of bfr2+/pad1+/sks1+ or pap1+ genes of Schizosaccharomyces pombe. Overexpression of bfr2+ did not confer multidrug resistance on a pap1-disrupted strain. In a mutant with bfr1+ (a putative membrane transporter which belongs to the ATP-binding cassette superfamily) disrupted, overexpression of either bfr2+ or pap1+ did not confer multidrug resistance. These findings suggest that bfr1+ acts as the most downstream effector of the multidrug resistance conferred by bfr2+ and pap1+ genes.
Arioka
M
M
Department of Biotechnology, University of Tokyo, Japan. arioka@hongo.ecc.u-tokyo.ac.jp
Kouhashi
M
M
Yoda
K
K
Takatsuki
A
A
Yamasaki
M
M
Kitamoto
K
K
D020168:ATP Binding Cassette Transporter, Subfamily B, Member 1 / Q000096:biosynthesis / Q000235:genetics* / Q000502:physiology; D000900:Anti-Bacterial Agents / Q000494:pharmacology; D000935:Antifungal Agents / Q000494:pharmacology*; D020126:Brefeldin A; D002227:Carbazoles / Q000494:pharmacology; D003517:Cyclopentanes / Q000494:pharmacology*; D003571:Cytochalasin B / Q000494:pharmacology; D003609:Dactinomycin / Q000494:pharmacology; D018432:Drug Resistance, Multiple / Q000235:genetics*; D004357:Drug Synergism; D004791:Enzyme Inhibitors / Q000494:pharmacology; D005656:Fungal Proteins / Q000096:biosynthesis / Q000235:genetics* / Q000502:physiology; D015966:Gene Expression Regulation, Fungal / Q000235:genetics*; D026121:Indole Alkaloids; D018942:Macrolides; D009183:Mycotoxins / Q000494:pharmacology; D000073718:Pancreatitis-Associated Proteins; D010641:Phenotype; D011493:Protein Kinase C / Q000037:antagonists & inhibitors; D011500:Protein Synthesis Inhibitors / Q000494:pharmacology; D012097:Repressor Proteins / Q000096:biosynthesis / Q000235:genetics* / Q000502:physiology; D029701:Saccharomyces cerevisiae Proteins; D012568:Schizosaccharomyces / Q000187:drug effects* / Q000235:genetics / Q000502:physiology; D017422:Sequence Analysis, DNA; D019311:Staurosporine / Q000494:pharmacology
D020168:ATP Binding Cassette Transporter, Subfamily B, Member 1; D000900:Anti-Bacterial Agents; D000935:Antifungal Agents; C089100:BFR1 protein, S cerevisiae; D002227:Carbazoles; D003517:Cyclopentanes; D004791:Enzyme Inhibitors; D005656:Fungal Proteins; D026121:Indole Alkaloids; D018942:Macrolides; D009183:Mycotoxins; D000073718:Pancreatitis-Associated Proteins; D011500:Protein Synthesis Inhibitors; C000612141:REG3A protein, human; D012097:Repressor Proteins; D029701:Saccharomyces cerevisiae Proteins; D003609:Dactinomycin; D020126:Brefeldin A; D003571:Cytochalasin B; C049985:staurosporine aglycone; D011493:Protein Kinase C; D019311:Staurosporine