Impact of immunoadsorption on complement activation, immunopathology, and hepatic perfusion during xenogeneic pig liver perfusion.
BACKGROUND
The impact of antibody adsorption by immunoapheresis on liver damage, complement activation, and hepatic perfusion was evaluated against the background of an application in extracorporeal pig liver perfusion for hepatic coma.


METHODS
Eighteen pig livers were ex vivo perfused close to physiological conditions with fresh human blood for 4 hr. The influence of the perfusion circuit was investigated by perfusions of the circuit in the absence of livers (group 1 [G1]; n=5). Livers were xenoperfused without modifications in group 2 (G2; n=6). In group 3 (G3; n=6), pure Sepharose columns were used prior to liver perfusion. Immunoapheresis with Ig-Therasorb 100 columns was used in group 4 (G4; n=6).


RESULTS
IgG was reduced by 95%, IgM by 72%, and IgA by 82% in G4, but only by about 30% in G3 (P<0.05). C4d, Bb fragment, and C3a levels were significantly lower in G4 than in G3 and G2 (P<0.05) after 180 min. Immunoadsorption diminished antibody and complement deposition as well as hepatocellular damage in G4. Portal angiographies demonstrated improved hepatic perfusion in G4.


CONCLUSIONS
Immunoapheresis reduced organ damage as well as complement activation and improved hepatic perfusion during xenogeneic pig liver perfusion.
Pascher
A
A
Institute for Surgical Research, Klinikum Grosshadern, Ludwig Maximilians University Munich, Germany.
Stangl
M
M
Hammer
C
C
D000208:Acute Disease; D000818:Animals; D000906:Antibodies / Q000378:metabolism; D003167:Complement Activation; D003165:Complement System Proteins / Q000378:metabolism*; D005112:Extracorporeal Circulation; D006084:Graft Rejection; D006801:Humans; D007163:Immunosorbent Techniques; D008099:Liver / Q000098:blood supply* / Q000276:immunology; D010477:Perfusion; D013552:Swine
D000906:Antibodies; D003165:Complement System Proteins