Protective effects of Mu-Fang-Ji-Tang against myocardial injury in a murine model of congestive heart failure induced by viral myocarditis.
The effects of Mu-Fang-Ji-Tang (TJ-36), a traditional Chinese herbal medicine, were studied in a murine model of congestive heart failure induced by viral myocarditis. In the group of animals treated with Mu-Fang-Ji-Tang in a dose of 1.5g/kg/day, the heart weight to body weight ratio was significantly lower than in the control group (p<0.01). Histopathological grades were also significantly lower in the Mu-Fang-Ji-Tang treated group than in the placebo group (p<0.05). Furthermore, survival was increased in the Mu-Fang-Ji-Tang treated group, versus the control group (p<0.05). In vitro, murine J774A.1 macrophages inoculated with encephalomyocarditis virus produced a significantly greater amount of nitrites compared to non-activated macrophages. Mu-Fang-Ji-Tang added to the cells (25, 50, 75, 100 microg/ml) concomitantly with the encephalomyocarditis virus inhibited nitrite formation in a concentration-dependent manner. Mu-Fang-Ji-Tang showed a protective effect against myocardial injury leading to congestive heart failure in this animal model.
Wang
W Z
WZ
Department of Cardiovascular Medicine, Kyoto University, Japan.
Matsumori
A
A
Matoba
Y
Y
Matsui
S
S
Sato
Y
Y
Hirozane
T
T
Shioi
T
T
Sasayama
S
S
D000818:Animals; D001835:Body Weight / Q000187:drug effects; D002460:Cell Line; D004195:Disease Models, Animal; D004365:Drugs, Chinese Herbal / Q000627:therapeutic use*; D006333:Heart Failure / Q000209:etiology* / Q000378:metabolism / Q000517:prevention & control*; D008264:Macrophages / Q000187:drug effects / Q000378:metabolism; D008297:Male; D051379:Mice; D008811:Mice, Inbred DBA; D009000:Monocytes / Q000187:drug effects / Q000378:metabolism; D009205:Myocarditis / Q000150:complications* / Q000378:metabolism / Q000821:virology; D009206:Myocardium / Q000473:pathology; D009569:Nitric Oxide / Q000096:biosynthesis; D009573:Nitrites / Q000378:metabolism; D009929:Organ Size / Q000187:drug effects
D004365:Drugs, Chinese Herbal; D009573:Nitrites; C099755:moku-boi-to; D009569:Nitric Oxide