The deduced primary structure of a ribosomal protein S4 from Trypanosoma cruzi.
Two cDNA clones encoding a protein homologous to ribosomal protein S4 from the protozoan parasite Trypanosoma cruzi were isolated and characterized. Both clones potentially encode for an identical basic protein of 273 amino acids. Sequence comparisons with other species indicate that this protein is highly conserved. Hybridization studies are consistent with the occurrence of two genes in T. cruzi encoding this ribosomal protein. An RNA of approximately 1 kb was present in both exponentially growing and stationary phase derived epimastigotes, and no significant differences were detected in its steady state concentration.
Hernández
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R
Department of Molecular Biology, Universidad Nacional Autónoma de México (UNAM), México, DF, Mexico. robher@servidor.unam.mx
Palacios
S
S
Herrera
J
J
Martínez-Calvillo
S
S
López
I
I
D000595:Amino Acid Sequence; D000818:Animals; D002874:Chromosome Mapping; D003001:Cloning, Molecular; D017124:Conserved Sequence; D018076:DNA, Complementary; D015723:Gene Library; D006801:Humans; D008969:Molecular Sequence Data; D015800:Protozoan Proteins / Q000096:biosynthesis / Q000737:chemistry / Q000235:genetics; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000737:chemistry; D012269:Ribosomal Proteins / Q000096:biosynthesis / Q000737:chemistry* / Q000235:genetics*; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid; D014349:Trypanosoma cruzi / Q000235:genetics* / Q000378:metabolism
D018076:DNA, Complementary; D015800:Protozoan Proteins; D011994:Recombinant Proteins; D012269:Ribosomal Proteins; C031798:ribosomal protein S4