Mapping of 22 new ESTs around a tumor suppressor gene and a senescence gene at 6q16-->q21.
Twenty two expressed sequence tags (ESTs) have been mapped at the border of 6q16-->q21 and at the proximal end of 6q21, a candidate for two tumor suppressor genes and a senescence gene. Use of a translocation and deletion hybrid panel together with a 4-Mb YAC contig allowed us to precisely define the position of the ESTs. Thirteen ESTs were placed within the 4-Mb interval at the proximal portion of 6q21 using a restriction map of the YAC contig, seven ESTs span a 2-Mb region on the 6q16-->q21 border, and two are distal to the contig. Refinement of the localization of these ESTs will provide substantial assistance in identifying new genes within the region 6q16-->q21.
Morelli
C
C
Department of Experimental and Diagnostic Medicine, University of Ferrara, Italy.
Cardona
F
F
Boyle
J M
JM
Negrini
M
M
Barbanti-Brodano
G
G
D000375:Aging / Q000235:genetics*; D002874:Chromosome Mapping; D018244:Chromosomes, Artificial, Yeast; D002896:Chromosomes, Human, Pair 6; D017931:DNA Primers; D017353:Gene Deletion; D016147:Genes, Tumor Suppressor / Q000235:genetics*; D005819:Genetic Markers; D006801:Humans; D014178:Translocation, Genetic
D017931:DNA Primers; D005819:Genetic Markers