Improved high-performance liquid chromatographic separation of peptidoglycan isolated from various Staphylococcus aureus strains for mass spectrometric characterization.
Reversed-phase high-performance liquid chromatography (RP-HPLC) of muropeptides, obtained by muramidase digestion of peptidoglycan in combination with amino acid analysis and plasma desorption time-of-flight mass spectrometry is today by far the best tool to analyze the fine structure of the peptidoglycans. Here we report further improvements of the RP-HPLC separation of muropeptides for analyzing the peptidoglycans of various methicillin-resistant strains of Staphylococcus aureus, with emphasis on a more detailed characterization of the interpeptide bridge of the peptidoglycans of this species.
Roos
M
M
Robert Koch Institute, Berlin, Germany.
Pittenauer
E
E
Schmid
E
E
Beyer
M
M
Reinike
B
B
Allmaier
G
G
Labischinski
H
H
D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D013058:Mass Spectrometry / Q000379:methods; D010457:Peptidoglycan / Q000737:chemistry / Q000302:isolation & purification*; D015203:Reproducibility of Results; D013211:Staphylococcus aureus / Q000737:chemistry*; D013696:Temperature
D010457:Peptidoglycan