[Effect of inhibitors of mitochondrial oxidative metabolism on Ca-responses induced by purinergic agonists and thapsigargin in rat peritoneal macrophages].
Effects of two metabolic inhibitors, oligomycin and carbonyl cyanide m-fluorophenylhydrazone (FCCP), on Ca2+ signals induced by purinergic agonists and thapsigargin in Fura-2-loaded rat peritoneal macrophages was investigated. 1 microgram/ml oligomycin or 1 microM FCCP were shown to inhibit 200 microM ATP or 200 microM UTP-evoked Ca2+ entry in macrophages. Independently of their chemical structure and site of inhibition, both metabolic poisons also inhibit the store-dependent or "capacitative" Ca2+ influx stimulated by emptying the intracellular Ca2+ stores with endoplasmic Ca(2+)-ATPase inhibitor thapsigargin (0.5 microM). These data are compatible with the important role the energy level of the cell plays in the control of Ca2+ entry in rat peritoneal macrophages.
Krutetskaia
Z I
ZI
Physiological Research Institute, St.Petersburg University.
Lebedev
O E
OE
Krutetskaia
N I
NI
D000818:Animals; D001692:Biological Transport / Q000187:drug effects; D002118:Calcium / Q000502:physiology*; D002259:Carbonyl Cyanide p-Trifluoromethoxyphenylhydrazone / Q000494:pharmacology*; D004791:Enzyme Inhibitors / Q000494:pharmacology*; D005456:Fluorescent Dyes; D016257:Fura-2; D017737:Macrophages, Peritoneal / Q000187:drug effects* / Q000648:ultrastructure; D008928:Mitochondria / Q000187:drug effects*; D009840:Oligomycins / Q000494:pharmacology*; D010084:Oxidation-Reduction; D010085:Oxidative Phosphorylation / Q000187:drug effects; D058913:Purinergic Agonists; D051381:Rats; D015398:Signal Transduction / Q000502:physiology*; D019284:Thapsigargin / Q000494:pharmacology
D004791:Enzyme Inhibitors; D005456:Fluorescent Dyes; D009840:Oligomycins; D058913:Purinergic Agonists; D002259:Carbonyl Cyanide p-Trifluoromethoxyphenylhydrazone; D019284:Thapsigargin; D002118:Calcium; D016257:Fura-2
Vliianie ingibitorov okislitel'nogo metabolizma mitokhondriĭ na Ca-otvety, indutsirovannye purinergicheskimi agonistami i tapsigarginom v peritoneal'nykh makrofagakh krysy.