Pregnancy after human donor oocyte cryopreservation and thawing in association with intracytoplasmic sperm injection in a patient with ovarian failure.
OBJECTIVE
To describe a pregnancy after human donor oocyte cryopreservation in association with intracytoplasmic sperm injection in a patient with ovarian failure.


METHODS
Department of Reproductive Medicine, CER Medical Institute, Buenos Aires, Argentina.


METHODS
A 48-year-old patient with ovarian failure.


RESULTS
Ten donated oocytes were cryopreserved. Survival after thawing was 30%. Three oocytes were microinjected, and two embryos were obtained. The fertilization rate was 66%, and embryo development was 100%. Both embryos were transferred to a patient who had received hormonal replacement therapy. The attempt was successful, and a pregnancy was achieved after the transfer.


CONCLUSIONS
In association with intracytoplasmic sperm injection, an adequate technique of freezing and thawing of human oocytes might achieve encouraging results in ART programs.
Polak de Fried
E
E
Department of Reproductive Medicine, CER Medical Institute, Buenos Aires, Argentina.
Notrica
J
J
Rubinstein
M
M
Marazzi
A
A
Gómez Gonzalez
M
M
D000328:Adult; D018997:Chorionic Gonadotropin, beta Subunit, Human / Q000097:blood; D015925:Cryopreservation; D004624:Embryo Transfer; D005260:Female; D005307:Fertilization in Vitro / Q000379:methods*; D006358:Hot Temperature; D006801:Humans; D007247:Infertility, Female / Q000628:therapy*; D008297:Male; D008845:Microinjections; D008875:Middle Aged; D018587:Oocyte Donation; D011247:Pregnancy; D011256:Pregnancy Outcome
D018997:Chorionic Gonadotropin, beta Subunit, Human