The isiB gene encoding flavodoxin is not essential for photoautotrophic iron limited growth of the cyanobacterium Synechocystis sp. strain PCC 6803.
When iron becomes limiting, Synechocystis 6803 induces the synthesis of flavodoxin. As a basis for genetic analysis, the flavodoxin-encoding isiB gene of Synechocystis 6803 was cloned and sequenced. The isiB gene was disrupted by insertion of an interposon within the isiB coding region resulting in two Synechocystis 6803 mutant strains, CKF-I and CKF-II. They were distinguished from each other by the orientation of the kanamycin resistance cassette. Photoautotrophic growth of the mutant strains under iron limiting conditions, which are sufficient for induction of flavodoxin in the wild-type cells, demonstrated that IsiB was not essential for Synechocystis 6803.
Kutzki
C
C
Botanisches Institut, Universität Bonn, Germany.
Masepohl
B
B
Böhme
H
H
D001426:Bacterial Proteins / Q000235:genetics* / Q000378:metabolism; D015153:Blotting, Western; D003001:Cloning, Molecular; D000458:Cyanobacteria / Q000235:genetics* / Q000254:growth & development* / Q000378:metabolism; D005418:Flavodoxin / Q000235:genetics* / Q000378:metabolism; D005798:Genes, Bacterial; D007501:Iron / Q000378:metabolism*; D016254:Mutagenesis, Insertional; D010957:Plasmids; D017422:Sequence Analysis, DNA
D001426:Bacterial Proteins; D005418:Flavodoxin; D007501:Iron