Intracellular mapping of 4'-deoxy-4'-iododoxorubicin in sensitive and multidrug resistant cells by electron spectroscopic imaging.
Electron spectroscopic imaging (ESI) was employed to study, with high spatial resolution, the intracellular distribution of the halogenated derivative of doxorubicin 4'-deoxy-4'-iododoxorubicin (IDX) in sensitive and multidrug resistant human breast carcinoma cells. Both ESI and electron energy loss spectroscopy (EELS) observations confirmed results obtained with flow cytometry (FC), laser scanning confocal microscopy (LSCM) and secondary ion mass spectrometry (SIMS). Moreover, ESI allowed us to obtain a more detailed intracellular localization of IDX. Our results confirm that nuclear DNA represents the main intracellular target for IDX and that the Golgi apparatus is involved in the intracellular transport of the drug.
Diociaiuti
M
M
Laboratorio di Ultrastrutture, Istituto Superiore di Sanità, Roma, Italy.
Calcabrini
A
A
Meschini
S
S
Arancia
G
G
D001943:Breast Neoplasms / Q000188:drug therapy*; D002277:Carcinoma / Q000188:drug therapy*; D002467:Cell Nucleus / Q000378:metabolism; D004247:DNA / Q000187:drug effects; D004317:Doxorubicin / Q000031:analogs & derivatives* / Q000378:metabolism; D018432:Drug Resistance, Multiple; D004577:Electron Probe Microanalysis; D005260:Female; D005434:Flow Cytometry; D006056:Golgi Apparatus / Q000378:metabolism; D006801:Humans; D018613:Microscopy, Confocal; D008854:Microscopy, Electron; D018629:Spectrometry, Mass, Secondary Ion; D014407:Tumor Cells, Cultured
C053044:4'-deoxy-4'-iododoxorubicin; D004317:Doxorubicin; D004247:DNA