Immunohistologic assessment of technetium-99m-MIBI uptake in benign and malignant breast lesions.
This study was undertaken to assess the relationship between the degree of 99mTc-MIBI uptake in breast lesions and the following histologic factors: neovascularity, desmoplastic reaction, cellular proliferation and mitochondrial density.


METHODS
Forty-two patients who previously underwent MIBI breast imaging (4 false-negative, 12 false-positive, 15 true-negative, 11 true-positive) were studied. Immunohistochemical staining was performed for neovascularity (Factor VIII antigen), desmoplasia (alpha-actin antigen), mitochondrial density (mitochondrial antigen) and cellular proliferation (MIB-1 antigen). The degree of microscopic staining was correlated with region of interest measurements of MIBI uptake on scintigraphy.


RESULTS
There was a poor correlation between MIBI uptake and the degrees of neovascularity (r = 0.08, p > 0.05) and intracellular mitochondrial density (r = 0.04, p > 0.05) while there was a moderate correlation with cellular proliferation (r = 0.4, p < 0.05) and desmoplasia (r = 0.55, p < 0.001).


CONCLUSIONS
The degree of MIBI uptake in breast lesions is multifactorial, but it appears to be related more to the degree of desmoplastic activity and cellular proliferation than neovascularity and mitochondrial density.
Cutrone
J A
JA
Department of Radiology, Harbor-UCLA Medical Center, Torrance, California, USA.
Yospur
L S
LS
Khalkhali
I
I
Tolmos
J
J
Devito
A
A
Diggles
L
L
Vargas
M P
MP
Shitabata
P
P
French
S
S
D001940:Breast / Q000000981:diagnostic imaging / Q000378:metabolism / Q000473:pathology; D001941:Breast Diseases / Q000175:diagnosis / Q000000981:diagnostic imaging*; D001943:Breast Neoplasms / Q000175:diagnosis / Q000000981:diagnostic imaging*; D005260:Female; D006801:Humans; D009389:Neovascularization, Pathologic / Q000473:pathology; D011877:Radionuclide Imaging; D019275:Radiopharmaceuticals; D017256:Technetium Tc 99m Sestamibi
D019275:Radiopharmaceuticals; D017256:Technetium Tc 99m Sestamibi