title: N-linked glycosylation of Drosophila rhodopsin occurs exclusively in the amino-terminal domain and functions in rhodopsin maturation.
abstract: Immature Drosophila rhodopsin is N-glycosylated, but undergoes complete deglycosylation during the process of protein maturation. In order to elucidate the site of glycosylation and its role in rhodopsin synthesis, we investigated the in vitro and in vivo synthesis of rhodopsin whose putative N-glycosylation sites (Asn-20 and Asn-196) were replaced by isoleucine. The results demonstrated that immature rhodopsin binds a single oligosaccharide chain exclusively at Asn-20 in the N-terminal extracellular domain. Furthermore, the results gave the first evidence directly indicating that deletion of the oligosaccharide chain markedly impedes rhodopsin maturation.
authors.affiliation: Department of Biology, Graduate School of Science, Osaka University, Toyonaka, Japan.
authors.forename: K
authors.initials: K
authors.lastname: Katanosaka
authors.forename: F
authors.initials: F
authors.lastname: Tokunaga
authors.forename: S
authors.initials: S
authors.lastname: Kawamura
authors.forename: K
authors.initials: K
authors.lastname: Ozaki
mesh_terms: D000581:Amidohydrolases / Q000378:metabolism; D000818:Animals; D030801:Animals, Genetically Modified; D002338:Carotenoids / Q000378:metabolism; D029721:Drosophila Proteins; D004331:Drosophila melanogaster / Q000737:chemistry* / Q000235:genetics; D005136:Eye Proteins / Q000235:genetics / Q000378:metabolism; D006031:Glycosylation; D009154:Mutation; D009844:Oligosaccharides / Q000378:metabolism; D043524:Peptide-N4-(N-acetyl-beta-glucosaminyl) Asparagine Amidase; D014176:Protein Biosynthesis; D011817:Rabbits; D012156:Reticulocytes; D012243:Rhodopsin / Q000235:genetics* / Q000378:metabolism*; D017299:Rod Opsins / Q000096:biosynthesis
chemical_list: D029721:Drosophila Proteins; D005136:Eye Proteins; D009844:Oligosaccharides; D017299:Rod Opsins; C083315:ninaE protein, Drosophila; D002338:Carotenoids; D012243:Rhodopsin; D000581:Amidohydrolases; D043524:Peptide-N4-(N-acetyl-beta-glucosaminyl) Asparagine Amidase