title: Serotype 19A variants of the Spanish serotype 23F multiresistant clone of Streptococcus pneumoniae.
abstract: Multiply-antibiotic-resistant isolates of serogroup 19 Streptococcus pneumoniae, possessing altered penicillin-binding protein (PBP) 1A, 2B, and 2X genes that are indistinguishable from those of the Spanish multiresistant serogroup 23F clone, are now commonly encountered in Spain. Those isolates that have been serotyped express type 19F capsular polysaccharide. Serotyping of further isolates, and hybridization using a serotype 19F-specific probe, has shown that some of them are serotype 19A, rather than 19F. The Spanish multiresistant serotype 19A, 19F, and 23F multiresistant strains were all shown to be very closely related in overall genotype, as they were indistinguishable by REP-PCR and by the sequencing of internal fragments of three house-keeping genes. The serotype 19A multiresistant strains, like the serotype 19F multiresistant strains, therefore appear to be a serotype variant of the Spanish multiresistant serotype 23F clone, which presumably has arisen by recombination at the capsular locus.
authors.affiliation: Molecular Microbiology Group, School of Biological Sciences, University of Sussex, Brighton, UK.
authors.forename: T J
authors.initials: TJ
authors.lastname: Coffey
authors.forename: M C
authors.initials: MC
authors.lastname: Enright
authors.forename: M
authors.initials: M
authors.lastname: Daniels
authors.forename: P
authors.initials: P
authors.lastname: Wilkinson
authors.forename: S
authors.initials: S
authors.lastname: Berrón
authors.forename: A
authors.initials: A
authors.lastname: Fenoll
authors.forename: B G
authors.initials: BG
authors.lastname: Spratt
mesh_terms: D016667:Bacterial Capsules / Q000235:genetics; D001426:Bacterial Proteins; D002352:Carrier Proteins / Q000235:genetics; D016172:DNA Fingerprinting; D004352:Drug Resistance, Microbial / Q000235:genetics*; D018432:Drug Resistance, Multiple / Q000235:genetics*; D005798:Genes, Bacterial; D014644:Genetic Variation; D006602:Hexosyltransferases; D006801:Humans; D017720:Molecular Epidemiology; D002267:Muramoylpentapeptide Carboxypeptidase / Q000235:genetics; D046915:Penicillin-Binding Proteins; D010458:Peptidyl Transferases; D011008:Pneumococcal Infections / Q000453:epidemiology / Q000382:microbiology; D016133:Polymerase Chain Reaction; D012703:Serotyping; D013030:Spain / Q000453:epidemiology; D013045:Species Specificity; D013296:Streptococcus pneumoniae / Q000145:classification* / Q000187:drug effects / Q000235:genetics*
chemical_list: D001426:Bacterial Proteins; D002352:Carrier Proteins; D046915:Penicillin-Binding Proteins; D010458:Peptidyl Transferases; D006602:Hexosyltransferases; D002267:Muramoylpentapeptide Carboxypeptidase