title: [Evaluation of sensitivity, specificity and predictive value of six qualitative serological methods for the detection of Helicobacter pylori antibodies].
abstract: Screening tests for IG g antibodies against Helicobacter pylori are usefull for a long follow up of patients who were well eradicated. The aim of this study was to determinate and compared sensibility, specificity, positive and negative predictive value of six qualitative serological tests for IG g antibodies detection in the diagnosis of H. Pylori infections. Between May and October 1996 52 patients (30 males and 22 females; median age 42.4 years, range 21-68) with H. Pylori infection assessed on two antral and two corpus biopsies by means of Giema stain and a rapid urease test were tested for IG g antibodies detection. The serological tests used were: Inmunocomb II (Orgenics) Enzimo Inmuno Assay Inmunoadsorbent qualitative, Flex Pack (Smith Kline Diagnostics, Abbott) inmunocromatographic cualitative, Pylori Stat test (Biowhittaker) Enzimo Inmuno Assay (ELISA) qualitative, Premier (Meridian Diagnostics) Enzimo Inmuno Assay ELISA) qualitative. Pyloristest (Orion Diagnóstica) latex aglutination qualitative, H. Pylori (Bio Tre) Enzimo Inmuno Assay cualitative. 10 healthy subjects with negative gastric biopsies and negative rapid ureasa test were used as control group. The six evaluated serological tests have a comparable sensibility (89-95%) and specificity (77-83%) for the diagnosis of HP infection. The presence of specific HP antibodies in infected patients revealed a strong correlation with the histological demonstration of the microrganisms. We can recommend this qualitative serological tests due to their high sensibility and specificity, simplicity and low cost.
authors.affiliation: Servicio de Laboratorio, Endoscopía y Patología, Hospital Municipal de Gastroenterología Dr. Carlos Bonorino Udaondo.
authors.forename: J
authors.initials: J
authors.lastname: Doweck
authors.forename: C
authors.initials: C
authors.lastname: Quintana
authors.forename: A
authors.initials: A
authors.lastname: Barrios
authors.forename: L
authors.initials: L
authors.lastname: Monastra
authors.forename: G
authors.initials: G
authors.lastname: Lopetegui
authors.forename: O
authors.initials: O
authors.lastname: Zerbo
authors.forename: L
authors.initials: L
authors.lastname: Schenone
authors.forename: A
authors.initials: A
authors.lastname: Giordano
authors.forename: J
authors.initials: J
authors.lastname: Valero
authors.forename: Z
authors.initials: Z
authors.lastname: Kogan
authors.forename: M A
authors.initials: MA
authors.lastname: Bartellini
authors.forename: R
authors.initials: R
authors.lastname: Corti
mesh_terms: D000328:Adult; D000368:Aged; D000907:Antibodies, Bacterial / Q000302:isolation & purification*; D005260:Female; D016481:Helicobacter Infections / Q000175:diagnosis; D016480:Helicobacter pylori / Q000276:immunology*; D006801:Humans; D007074:Immunoglobulin G / Q000302:isolation & purification*; D008297:Male; D008875:Middle Aged; D011237:Predictive Value of Tests; D012680:Sensitivity and Specificity; D012698:Serologic Tests
chemical_list: D000907:Antibodies, Bacterial; D007074:Immunoglobulin G
vernacular_title: Evaluación de sensibilidad, especificidad y valor predictivo de seis métodos serológicos cualitativos para la detección de anticuerpos contra el Helicobacter Pylori.