Leukemia inhibitory factor maintains choline acetyltransferase expression in vivo.
Following axotomy most medial septal neurons in the adult rat brain have dramatically reduced numbers of choline acetyltransferase (ChAT) positive neurons. Since leukemia inhibitory factor (LIF) promotes cholinergic expression in several neuronal populations, the aim of this study was to determine if LIF would continue to support cholinergic expression in axotomized medial septal neurons. Mini-osmotic pumps were used to infuse saline or LIF into the lateral cerebral ventricle. Counts of ChAT and low-affinity nerve growth factor (p75NGFR) immunostained neurons indicated that LIF-treated animals retained ChAT expression in > 90% of axotomized neurons whereas in saline-infused animals this was < 30%. Also, LIF was equally effective in maintaining p75NGFR expression levels in axotomized medial septal neurons.
Cheema
S S
SS
Department of Anatomy, Monash University, Clayton, Victoria, Australia.
Arumugam
D
D
Murray
S S
SS
Bartlett
P F
PF
D000704:Analysis of Variance; D000818:Animals; D019771:Axotomy; D002795:Choline O-Acetyltransferase / Q000096:biosynthesis*; D015536:Down-Regulation; D006131:Growth Inhibitors / Q000494:pharmacology*; D015918:Infusion Pumps, Implantable; D015850:Interleukin-6; D053605:Leukemia Inhibitory Factor; D008222:Lymphokines / Q000494:pharmacology*; D008297:Male; D009474:Neurons / Q000187:drug effects* / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D020800:Receptor, Nerve Growth Factor; D017475:Receptors, Nerve Growth Factor / Q000378:metabolism*; D012688:Septum Pellucidum / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism
D006131:Growth Inhibitors; D015850:Interleukin-6; D053605:Leukemia Inhibitory Factor; D008222:Lymphokines; D020800:Receptor, Nerve Growth Factor; D017475:Receptors, Nerve Growth Factor; D002795:Choline O-Acetyltransferase