A strain device imposing dynamic and uniform equi-biaxial strain to cultured cells.
The objective of this study is to design a new apparatus to allow the control of the magnitude and frequency of dynamic stretch applied uniformly to cells cultured on a silicon elastic membrane. The apparatus is designed to produce equi-biaxial dynamic stretches with area changes ranging from 0% to 55% and frequencies ranging from 0 to 2 Hz. Homogeneous finite strain analysis using triangles of markers was performed to compute the symmetric two-dimensional Lagrangian strain tensor on the membrane. Measurements of strain in both static and dynamic conditions showed that the shear component of the strain tensor (Erc) was near zero, and that there was no significant difference between radial (Err) and circumferential (Ecc) components, indicating the attainment of equi-biaxial strain. Bovine aortic endothelial cells were transiently transfected with a chimeric construct in which the luciferase reporter is driven by TPA-responsive elements (TRE). The transfected cells cultured on the membrane were stretched. The luciferase activity increased significantly only when the cells were stretched by 15% or more in area. Cells in different locations of the membrane showed similar induction of luciferase activities, confirming that strain is uniform and equi-biaxial across the membrane.
Sotoudeh
M
M
Department of Bioengineering and Institute for Biomedical Engineering, University of California, San Diego, La Jolla 92093-0412, USA.
Jalali
S
S
Usami
S
S
Shyy
J Y
JY
Chien
S
S
D000818:Animals; D001698:Biomedical Engineering / Q000295:instrumentation*; D002417:Cattle; D002478:Cells, Cultured; D004548:Elasticity; D004730:Endothelium, Vascular / Q000166:cytology / Q000378:metabolism; D004867:Equipment Design; D017930:Genes, Reporter; D008156:Luciferases / Q000235:genetics; D012825:Silicon; D013314:Stress, Mechanical; D018808:Transcription Factor AP-1 / Q000378:metabolism; D015533:Transcriptional Activation; D014162:Transfection
D018808:Transcription Factor AP-1; D008156:Luciferases; D012825:Silicon