A new prenylated flavone from Artocarpus champeden inhibits the K(+)-dependent amino acid transport in Bombyx mori midgut.
The effect of some flavonoids on the K(+)-dependent and K(+)-independent leucine uptake into brush border membrane vesicles from Bombyx mori larval midgut was investigated. Among the compounds tested, cyclochampedol, recently purified from Artocarpus champeden, was able to inhibit in micromolar range the leucine transport. The inhibition occurred both in the absence and in the presence of potassium and was not affected by leucine concentration. The apparent Ki was 0.25 mM. Cyclochampedol represents the first non-competitive inhibitor of an amino acid transport system in Lepidoptera. The relevance of this result is discussed.
Parenti
P
P
Dipartimento di Fisiologia e Biochimica Generali, Università degli Studi di Milano, Italy. paolopa@imiucca.csi.unimi.it
Pizzigoni
A
A
Hanozet
G
G
Hakim
E H
EH
Makmur
L
L
Achmad
S A
SA
Giordana
B
B
D000596:Amino Acids / Q000378:metabolism*; D000818:Animals; D001693:Biological Transport, Active / Q000187:drug effects; D012831:Bombyx / Q000187:drug effects / Q000378:metabolism; D004064:Digestive System / Q000187:drug effects / Q000378:metabolism; D047309:Flavones; D005419:Flavonoids / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D007700:Kinetics; D007814:Larva / Q000187:drug effects / Q000378:metabolism; D007930:Leucine / Q000378:metabolism; D008871:Microvilli / Q000187:drug effects / Q000378:metabolism; D015394:Molecular Structure; D011188:Potassium / Q000378:metabolism*; D017368:Protein Prenylation; D014197:Trees / Q000737:chemistry
D000596:Amino Acids; D047309:Flavones; D005419:Flavonoids; C111536:artocarpin flavonoid; C111535:cyclochampedol; D007930:Leucine; D011188:Potassium