[The effect of nocodazole on the redistribution of phosphoinositide-specific phospholipase C gamma 1 during mitogenic signal transduction in A-431 cells].
Phosphoinositide-specific phospholipase C gamma 1 is associated with EGF receptor in A-431 cells after EGF treatment. It is shown that phospholipase C gamma 1 is co-localized with internalized receptors as well as with membrane ones during receptor-mediated endocytosis. Nocodazole is known as an inhibitor of microtubule assembly, thus leading to a complete disappearance of microtubule network. Nocodazole had no effect on phospholipase C gamma 1--EGF receptor association and co-localization, but the intracellular distribution of both the proteins differed dramatically. Phospholipase C gamma 1 and EGF receptor were localized in endosomes in the periphery of the cell. Besides, pretreatment of A-431 cells with nocodazole resulted in decreasing tyrosine phosphorylation of some proteins. These data suggest that the internalized receptor may serve as an additional starting point for triggering cell signalling.
Medvedeva
N D
ND
Institute of Cytology, Russian Academy of Sciences, St. Petersburg.
Chupreta
S V
SV
D'iakonova
M Iu
MIu
Tvorogov
D B
DB
Blagoveshchenskaia
A D
AD
Tsupkina
N V
NV
Nikol'skiĭ
N N
NN
D000970:Antineoplastic Agents / Q000494:pharmacology*; D002294:Carcinoma, Squamous Cell / Q000378:metabolism* / Q000473:pathology; D004815:Epidermal Growth Factor / Q000494:pharmacology; D066246:ErbB Receptors / Q000187:drug effects / Q000378:metabolism; D006801:Humans; D007527:Isoenzymes / Q000187:drug effects* / Q000378:metabolism; D008870:Microtubules / Q000187:drug effects / Q000201:enzymology; D015739:Nocodazole / Q000494:pharmacology*; D010716:Phosphatidylinositols / Q000378:metabolism*; D051966:Phospholipase C gamma; D011975:Receptors, Mitogen / Q000187:drug effects / Q000378:metabolism; D015398:Signal Transduction / Q000187:drug effects*; D013379:Substrate Specificity / Q000187:drug effects; D014407:Tumor Cells, Cultured; D010738:Type C Phospholipases / Q000187:drug effects* / Q000378:metabolism
D000970:Antineoplastic Agents; D007527:Isoenzymes; D010716:Phosphatidylinositols; D011975:Receptors, Mitogen; D004815:Epidermal Growth Factor; D066246:ErbB Receptors; D010738:Type C Phospholipases; D051966:Phospholipase C gamma; D015739:Nocodazole
Vliianie nokodazola na pereraspredelenie fosfoinozitid-spetsificheskoŏi fosfalipazy C gamma 1 v protsesse peredachi mitogennogo signala v kletkakh A-431.