title: Analysis of chromosome aberrations in human peripheral lymphocytes induced by 5.4 keV x-rays.
abstract: Irradiation of human lymphocytes by x-rays has been seen, in past studies, to produce increasing frequencies of chromosome aberrations at lower x-ray energies. However, in one earlier irradiation experiment with chromium x-rays, the relative biological effectiveness (RBE) did not appear to be larger than that of hard x-rays, especially at higher doses. A possible reason for this unexpected result may have been the irradiation and culture conditions. We have, therefore, in the present study used a technique that has been developed in our laboratory to ensure uniformity of irradiation within lymphocytes and to avoid artefacts due to the cell cycle kinetics. Monolayers of 3-h-stimulated lymphocytes were exposed to 5.4 keV x-rays. A linear-quadratic dose-response was found for dicentrics. The comparison to an earlier finding with 220 kV x-rays shows the expected result of the RBE of the 5.4 keV x-rays to be above that of 220 kV x-rays. The intercellular distribution of dicentrics did not differ significantly from a Poisson distribution.
authors.affiliation: Radiobiological Institute, University of Munich, Germany.
authors.forename: H
authors.initials: H
authors.lastname: Roos
authors.forename: E
authors.initials: E
authors.lastname: Schmid
mesh_terms: D002478:Cells, Cultured; D002842:Chromatids / Q000528:radiation effects; D002869:Chromosome Aberrations; D004307:Dose-Response Relationship, Radiation; D006801:Humans; D008214:Lymphocytes / Q000528:radiation effects*; D008297:Male; D014965:X-Rays