title: Generation and characterization of a polyclonal antipeptide antibody to human glycodelin.
abstract: OBJECTIVE
  To develop and characterize an antiglycodelin antibody using a 15-amino acid synthetic peptide as antigen, derived from the sequence of human glycodelin.
  
  
  METHODS
  We have developed a chicken antiglycodelin-derived peptide antibody and have characterized the antibody with the use of endometrial and ovarian cell lines. The antibody was also tested for its ability to detect glycodelin by ELISA assay, immunocytochemistry, and by Western blot.
  
  
  METHODS
  Various cell lines, cell culture medium, and amniotic fluid were used in the experiments.
  
  
  METHODS
  Amniotic fluid was collected from pregnant patients in their first trimester of pregnancy.
  
  
  METHODS
  No intervention.
  
  
  METHODS
  Detection of glycodelin.
  
  
  RESULTS
  The cell lines RL95-2 (human endometrial carcinoma cells), OVCAR-3 (human ovarian adenocarcinoma cells), HeLa (human cervical epitheloid carcinoma cells), and EM42-D (human endometrial epithelial cells) reacted with the antibody, indicating the presence of glycodelin. A specific 45-kd protein representing glycodelin was detected by Western blot in the amniotic fluid.
  
  
  CONCLUSIONS
  Antipeptide antibodies can be successfully used to detect and quantify the presence of glycodelin in cells and fluids.
authors.affiliation: Department of Gynecology and Obstetrics, Emory University, Atlanta, Georgia 30322, USA.
authors.forename: A S
authors.initials: AS
authors.lastname: Poddar
authors.forename: J G
authors.initials: JG
authors.lastname: Kim
authors.forename: K P
authors.initials: KP
authors.lastname: Gill
authors.forename: B N
authors.initials: BN
authors.lastname: Bates
authors.forename: N
authors.initials: N
authors.lastname: Santanam
authors.forename: J A
authors.initials: JA
authors.lastname: Rock
authors.forename: A A
authors.initials: AA
authors.lastname: Murphy
authors.forename: S
authors.initials: S
authors.lastname: Parthasarathy
mesh_terms: D000230:Adenocarcinoma / Q000737:chemistry; D000653:Amniotic Fluid / Q000737:chemistry; D000818:Animals; D000906:Antibodies / Q000276:immunology*; D015153:Blotting, Western; D002460:Cell Line; D002645:Chickens / Q000276:immunology; D016889:Endometrial Neoplasms / Q000737:chemistry; D004717:Endometrium / Q000737:chemistry; D004797:Enzyme-Linked Immunosorbent Assay; D004847:Epithelial Cells / Q000737:chemistry; D005260:Female; D000071816:Glycodelin; D006023:Glycoproteins / Q000032:analysis / Q000276:immunology*; D006367:HeLa Cells / Q000737:chemistry; D006801:Humans; D007150:Immunohistochemistry; D010051:Ovarian Neoplasms / Q000737:chemistry; D011247:Pregnancy; D011257:Pregnancy Proteins / Q000032:analysis / Q000276:immunology*; D014407:Tumor Cells, Cultured
chemical_list: D000906:Antibodies; D000071816:Glycodelin; D006023:Glycoproteins; C045246:PAEP protein, human; D011257:Pregnancy Proteins