Frequency of human anti-FVIII antibodies in humanized SCID mice elicited by recombinant deleted factor VIII and by a plasma derived factor VIII.
SCID mice were grafted with human PBL (hu-PBL-SCID) from healthy or haemophilia A donors. Those containing human and no murine Ig in their plasma, were injected with 100 U VIII:Ag of a plasma derived (pd) FVIII or recombinant deleted Factor VIII (FVIII deltaII) and with 10 microg of tetanus toxoid as control immunogen. The frequency and the intensity of the humoral specific responses were measured in 253 mice humanized with PBL from 13 different donors. There was no significant difference in the frequency or intensity of the anti-FVIII immune responses to pd FVIII and FVIII deltaII. Neutralizing antibodies were only detected in the plasma of mice humanized with cells from haemophiliacs having FVIII inhibitors in their blood. The immune responses observed in hu-PBL-SCID mice correlated with the immune status of the corresponding human donor.
Laulan
A
A
Laboratoire Français du Fractionnement et des Biotechnologies, Les Ulis, Courtaboeuf, France.
Sauger
A
A
Germain
C
C
Montembault
A M
AM
Sans
I
I
Potentini-Esnault
C
C
Chicane
L
L
Gilard
C
C
Sultan
Y
Y
Bartholeyns
J
J
D000328:Adult; D000818:Animals; D000917:Antibody Formation; D005169:Factor VIII / Q000037:antagonists & inhibitors / Q000276:immunology*; D005260:Female; D006467:Hemophilia A / Q000097:blood / Q000628:therapy; D006801:Humans; D007114:Immunization; D008297:Male; D051379:Mice; D016513:Mice, SCID; D008875:Middle Aged; D011994:Recombinant Proteins / Q000276:immunology; D013745:Tetanus Toxoid / Q000276:immunology; D014183:Transplantation, Heterologous
D011994:Recombinant Proteins; D013745:Tetanus Toxoid; D005169:Factor VIII