A thermodynamic study of the binding of linear and cyclic oligosaccharides to the maltodextrin-binding protein of Escherichia coli.
Isothermal titration calorimetric (ITC) studies over a range of temperatures of the binding of maltose, maltotriose, maltotetraose and beta-cyclodextrin to the maltodextrin-binding protein (MBP) of Escherichia coli are reported. The binding constants of maltose, maltotriose and beta-cyclodextrin are not very different, namely 8.7 x 10(5), 13.0 x 10(5) and 2.55 x 10(5) M-1, respectively at 25 degrees C. The calorimetric data obtained with maltotetraose cannot be interpreted in terms of a definite binding constant. The binding of maltose and maltotriose is endothermic with a large entropy increase while that of beta-cyclodextrin is exothermic, with a smaller entropy increase. The binding of maltotetraose was endothermic or exothermic depending on the temperature.
Thomson
J
J
Agouron, San Diego, CA 92121, USA.
Liu
Y
Y
Sturtevant
J M
JM
Quiocho
F A
FA
D001426:Bacterial Proteins / Q000737:chemistry*; D002151:Calorimetry; D002352:Carrier Proteins / Q000737:chemistry* / Q000378:metabolism; D003505:Cyclodextrins / Q000378:metabolism; D004926:Escherichia coli / Q000737:chemistry*; D029968:Escherichia coli Proteins; D006593:Hexokinase / Q000378:metabolism; D008320:Maltose / Q000032:analysis / Q000378:metabolism; D009844:Oligosaccharides / Q000378:metabolism*; D033902:Periplasmic Binding Proteins; D011485:Protein Binding; D013696:Temperature; D013816:Thermodynamics; D047391:alpha-Cyclodextrins; D047392:beta-Cyclodextrins
C016242:AraF protein, E coli; D001426:Bacterial Proteins; D002352:Carrier Proteins; D003505:Cyclodextrins; D029968:Escherichia coli Proteins; C461619:MalE protein, E coli; D009844:Oligosaccharides; D033902:Periplasmic Binding Proteins; D047391:alpha-Cyclodextrins; D047392:beta-Cyclodextrins; D008320:Maltose; D006593:Hexokinase; C031215:betadex; C032613:alpha-cyclodextrin