[Methods of in vitro assessment of the degree of complement activation by the classical pathway].
A sensitive a simple method is proposed for assessing the complement activation degree by the monospecific classical route. The alternative pathway of the complement activation proposed by Adachi et al. in 1990 is taken account of. The specific features of the proposed method are 1) use of commercial dry complement of guinea pigs with the regulatory protein defect needed for the alternative pathway of complement activation; 2) absence of EGTA in GVB and presence of Ca2+; 3) use of sensitized sheep red blood cells as the target cells. These conditions prevent the alternative pathway of complement activation and permit only the classical pathway. Effects of some drugs on the classical pathway of complement activation by the new method and on Adachi's alternative method are investigated. Estradurin and fosfestrol affect the complement activation by both pathways.
Kovalev
B M
BM
Nazarova
I V
IV
Khotimchenko
Iu S
IuS
D000818:Animals; D000893:Anti-Inflammatory Agents / Q000494:pharmacology; D000936:Antigen-Antibody Complex / Q000276:immunology; D000970:Antineoplastic Agents / Q000494:pharmacology; D003170:Complement Pathway, Alternative / Q000187:drug effects; D003171:Complement Pathway, Classical / Q000187:drug effects; D003165:Complement System Proteins / Q000276:immunology; D004054:Diethylstilbestrol / Q000031:analogs & derivatives / Q000494:pharmacology; D004912:Erythrocytes / Q000276:immunology; D004958:Estradiol / Q000031:analogs & derivatives / Q000494:pharmacology; D045166:Estradiol Congeners / Q000494:pharmacology; D005069:Evaluation Studies as Topic; D006168:Guinea Pigs; D006801:Humans; D066298:In Vitro Techniques; D007202:Indicators and Reagents; D017258:Medroxyprogesterone Acetate / Q000494:pharmacology; D011239:Prednisolone / Q000494:pharmacology; D045167:Progesterone Congeners / Q000494:pharmacology; D011817:Rabbits; D012756:Sheep; D013053:Spectrophotometry; D015054:Zymosan / Q000494:pharmacology
D000893:Anti-Inflammatory Agents; D000936:Antigen-Antibody Complex; D000970:Antineoplastic Agents; D045166:Estradiol Congeners; D007202:Indicators and Reagents; D045167:Progesterone Congeners; D004958:Estradiol; D004054:Diethylstilbestrol; D003165:Complement System Proteins; D015054:Zymosan; D011239:Prednisolone; C004955:fosfestrol; D017258:Medroxyprogesterone Acetate; C008958:polyestradiol phosphate
Metodika opredeleniia in vitro stepeni aktivirovaniia komplementa po klassicheskomu puti.