Five monoclonal antibodies against glycophorin A of human erythrocyte recognize glycoprotein of bovine erythrocyte.
To study heterophile blood antigens on erythrocytes between human and experimental or domestic animals, we have produced 295 monoclonal antibodies (MAbs) to human erythrocyte membrane protein. According to the affinity, reactivity, and titre of the MAbs, we selected 40 clones to study the heterophile blood antigens between human and bovine, chicken, guinea pig, horse, rabbit, sheep, and swine. Five MAbs commonly reacted with human type A, type B, and type O erythrocytes and reacted with bovine erythrocytes as well but did not react with erythrocytes from other species. Other MAbs did not react with erythrocytes from all the tested animals. These five MAbs reacted with the same erythrocyte membrane protein, 90 KD glycophorin A (GPA) of human or 200 KD major glycoprotein and other two components of bovine by immunoblotting and GPA competitive inhibition assay. Furthermore, by enzyme treatment and monosaccharide competitive inhibition assay, it was confirmed that these five MAbs recognized antigen epitope of glycosylation free amino acid portion but not glycosylation portion of GPA of erythrocyte membrane.
Shan
B
B
Department of Immunology, University of Occupational and Environmental Health, School of Medicine, Kitakyushu, Japan.
Sugiura
T
T
Yamashita
U
U
D000017:ABO Blood-Group System / Q000276:immunology; D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D000918:Antibody Specificity; D002417:Cattle; D003429:Cross Reactions; D004912:Erythrocytes / Q000276:immunology*; D005577:Forssman Antigen / Q000276:immunology; D006021:Glycophorins / Q000276:immunology*; D006168:Guinea Pigs; D006736:Horses; D006801:Humans; D012756:Sheep; D013045:Species Specificity
D000017:ABO Blood-Group System; D000911:Antibodies, Monoclonal; D006021:Glycophorins; D005577:Forssman Antigen