The spatial organisation of the central nervous system of Mesocestoides corti as revealed by microinjection of carbocyanine dye.
The carbocyanine dyes DiI, DiA and DiO were microinjected into the cerebral ganglion of intact Mesocestoides corti tetrathyridia to determine the spatial organisation and connectivity patterns of the CNS. Of the dyes tested, DiI proved to be the most effective, giving highly fluorescent and persistent staining of even very fine calibre afferent and efferent nerve fibres. DiI labelling, in conjunction with transmission electron microscopy, revealed the nervous system to consist of sensory endings, directly connected to the cerebral ganglion by elongated cellular tracts, efferent nerve fibres which innervated the suckers, and longitudinal nerve cords which travelled along the remainder of the body.
Burns
A J
AJ
School of Applied Biological and Chemical Sciences, University of Ulster, County Londonderry, Northern Ireland, U.K. alburns@infobiogen.fr
Allen
J M
JM
McKerr
G
G
D000818:Animals; D002232:Carbocyanines; D005456:Fluorescent Dyes; D017952:Ganglia, Invertebrate / Q000033:anatomy & histology; D008645:Mesocestoides / Q000033:anatomy & histology*; D008845:Microinjections; D009420:Nervous System / Q000033:anatomy & histology*; D009434:Neural Pathways; D011984:Sensory Receptor Cells
D002232:Carbocyanines; D005456:Fluorescent Dyes; C024286:3,3'-dioctadecylindocarbocyanine