Rapid determination of trans-fatty acids in human adipose tissue. Comparison of attenuated total reflection infrared spectroscopy and gas chromatography.
A rapid attenuated total reflection (ATR) infrared (IR) spectroscopy procedure was used for quantitating the levels of total trans-fatty acid methyl ester (FAME) derivatives in neat (without solvent) test samples isolated from human adipose tissue. This procedure requires no weighing of the laboratory sample. The single-beam spectrum of the trans-containing FAMEs was 'ratioed' against that of a reference material having only cis double bonds in order to obtain a symmetric absorption band at 966 cm(-1) on a horizontal background. A single-reflection ATR diamond cell that requires only about 1 microl of neat FAMEs was used. The average level of trans-fatty acids in human adipose tissue found by ATR (3.07+/-0.27%) was generally higher than that obtained by gas chromatography (2.59+/-0.20%). Reasons for such a difference are discussed.
Fritsche
J
J
University of Hamburg, Institute of Biochemistry and Food Chemistry, Germany.
Steinhart
H
H
Mossoba
M M
MM
Yurawecz
M P
MP
Sehat
N
N
Ku
Y
Y
D000273:Adipose Tissue / Q000737:chemistry*; D000293:Adolescent; D002648:Child; D002675:Child, Preschool; D002849:Chromatography, Gas; D005227:Fatty Acids / Q000032:analysis; D005231:Fatty Acids, Unsaturated / Q000032:analysis*; D005260:Female; D006801:Humans; D007223:Infant; D007536:Isomerism; D008297:Male; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013055:Spectrophotometry, Infrared / Q000379:methods
D005227:Fatty Acids; D005231:Fatty Acids, Unsaturated