Infection with foot-and-mouth disease virus results in a rapid reduction of MHC class I surface expression.
The modulation of MHC class I molecule expression on the surface of cells as a consequence of foot-and-mouth disease virus (FMDV) infection has been examined. On cells infected with FMDV, class I expression was reduced to approximately 70% of the initial value 3 h after the infection and to 53% after 6 h. On cells depleted of surface class I complexes by acid treatment, the appearance of newly assembled class I-peptide complexes on the cell surface of non-infected cells increased immediately upon neutralization and original class I levels were recovered in about 20 h. In contrast, the appearance of new peptide-bound class I molecules on the cell surface was inhibited as early as 30 min after FMDV infection. Since the shut-down of FMDV-mediated host protein synthesis occurs approximately 2-3 h post-infection, this result suggests that an earlier event, which prevents the surface expression of newly synthesized complexes, is induced following FMDV infection. Thus, FMDV-infected cells rapidly become unable to present viral peptides in association with MHC class I molecules to T lymphocytes. Such a mechanism would assist virus evasion of the cytotoxic immune response of the host.
Sanz-Parra
A
A
Centro de Investigación en Sanidad Animal, INIA, Valdeolmos, Madrid, Spain.
Sobrino
F
F
Ley
V
V
D000818:Animals; D002460:Cell Line; D005434:Flow Cytometry; D005536:Foot-and-Mouth Disease / Q000276:immunology*; D015395:Histocompatibility Antigens Class I / Q000378:metabolism*; D000949:Histocompatibility Antigens Class II / Q000378:metabolism; D008264:Macrophages / Q000378:metabolism / Q000821:virology; D013552:Swine; D013997:Time Factors
D015395:Histocompatibility Antigens Class I; D000949:Histocompatibility Antigens Class II