Assay of S-ethyl-N-acetyl-l-cysteine in urine by high-performance liquid chromatography using post-column reaction detection.
The assay of the ethyl chloride metabolite S-ethyl-N-acetyl-L-cysteine in human urine by HPLC is described. The compound is enriched by adsorption on a non-polar adsorbent of graphitized non-porous carbon, and then stripped from positively charged compounds by application onto a strong acid cation-exchanger. Subsequently, an enzymatic deacetylation is carried out and the acylase is removed by centrifugal ultrafiltration. Separation of the sample is performed by cation-exchange chromatography applying an eluent of a very low elution strength (diluted formic acid). In the column effluent S-ethyl-L-cysteine is derivatized by o-phthaldialdehyde and the reaction product is detected by fluorescence measurement. In human urine a detection limit in the low ppb range is achieved.
Eskinja
M
M
Institut für Analytische Chemie der Universität Wien, Austria.
Lamprecht
G
G
Scherer
G
G
Schmid
E R
ER
D000107:Acetylation; D000111:Acetylcysteine / Q000031:analogs & derivatives* / Q000652:urine; D002273:Carcinogens / Q000378:metabolism; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D002852:Chromatography, Ion Exchange; D005018:Ethyl Chloride / Q000378:metabolism; D005561:Formates; D006801:Humans; D008623:Mercaptoethanol; D000432:Methanol; D012680:Sensitivity and Specificity; D012997:Solvents; D009764:o-Phthalaldehyde
D002273:Carcinogens; D005561:Formates; C110991:S-ethyl-N-acetyl-cysteine; D012997:Solvents; C030544:formic acid; D005018:Ethyl Chloride; D008623:Mercaptoethanol; D009764:o-Phthalaldehyde; D000111:Acetylcysteine; D000432:Methanol