Preliminary study on hydrophobic interaction chromatography of Chromobacterium viscosum lipase on polypropylene glycol immobilized on Sepharose.
The purification of Chromobacterium viscosum lipase was performed using a polypropylene glycol-Sepharose gel. The influence of the mobile phase composition on the chromatographic behaviour of Chromobacterium viscosum lipase was studied and it was found that the retention of lipase depends on the salt used and increased with ionic strength. Using 20% (w/v) ammonium sulphate in the eluent, a total retention of lipase on the column was obtained.
Diogo
M M
MM
Departamento de Química, Universidade da Beira Interior, Covilhã, Portugal.
Cabral
J M
JM
Queiroz
J A
JA
D001426:Bacterial Proteins / Q000302:isolation & purification*; D002853:Chromatography, Liquid / Q000379:methods*; D002861:Chromobacterium / Q000201:enzymology*; D008049:Lipase / Q000302:isolation & purification* / Q000378:metabolism; D011092:Polyethylene Glycols / Q000737:chemistry*; D012685:Sepharose / Q000737:chemistry*
D001426:Bacterial Proteins; D011092:Polyethylene Glycols; D012685:Sepharose; D008049:Lipase