[Defect of preferential repair of gamma-ray-induced single-strand breaks in transcribed and non-transcribed DNA in Cockayne syndrome cells].
The repair of gamma-ray-induced DNA single-strand breaks in transcribed (protooncogene c-myc) and non-transcribed (human satellite III) DNA of normal human fibroblasts and fibroblasts obtained from a patient with Cockayne's syndrome (CS) has been investigated. A method of alkaline sucrose sedimentation was applied besides the Southern hybridization of 32P-DNA, containing sequences analysed with total 3H-DNA distributed through sucrose gradient fractions. No increase in the induction of DNA single-strand breaks was found in gamma-irradiated CS fibroblasts, compared to normal human fibroblasts. At the same time, an evident defect in the preferential repair of single-strand breaks in c-myc gene was observed.
Igusheva
O A
OA
Institute of Cytology, Russian Academy of Sciences, St. Petersburg.
Mikhel'son
V M
VM
Pleskach
N M
NM
Bil'din
V N
VN
Zhestianikov
V D
VD
D015139:Blotting, Southern; D003057:Cockayne Syndrome / Q000235:genetics*; D004249:DNA Damage; D004260:DNA Repair; D004276:DNA, Satellite; D004277:DNA, Single-Stranded / Q000528:radiation effects*; D005347:Fibroblasts / Q000528:radiation effects; D005720:Gamma Rays; D016259:Genes, myc; D006801:Humans; D014158:Transcription, Genetic / Q000528:radiation effects*
D004276:DNA, Satellite; D004277:DNA, Single-Stranded
Defekt preimushchestvennoĭ reparatsii gamma-indutsirovanny odnonitevykh razryvov v transkribiruemoĭ i netranskribiruemoĭ DNK pri sindrome Kokeĭna.