title: Generation of mutant mice with large chromosomal deletion by use of irradiated ES cells--analysis of large deletion around hprt locus of ES cell.
abstract: A method of generating mice from embryonic stem (ES) cells with a large chromosomal deletion produced by X-ray irradiation has been developed. Fifty-two mutant ES clones were made that carried a nested set of chromosomal deletions up to approximately 10 cM in length around the hprt locus on the X Chromosome (Chr). Germline chimeras were generated from three ES clones with deletions ranging from 200 to 700 kb. In germline male mice from two independent clones, deletions around the hprt locus yielded a runty phenotype or caused death at birth. The runty mice had approximately 1/3 the body weight and size of wild littermates and did not survive more than 3 weeks after birth. The most plausible cause of these phenotypes is defects in regions flanking the hprt locus. This method of creating mutant mice with a large chromosomal deletion is very useful for the identification and understanding of gene functions.
authors.affiliation: Life Science Research Laboratory, Japan Tobacco Inc., Kanagawa, Japan.
authors.forename: A
authors.initials: A
authors.lastname: Kushi
authors.forename: K
authors.initials: K
authors.lastname: Edamura
authors.forename: M
authors.initials: M
authors.lastname: Noguchi
authors.forename: K
authors.initials: K
authors.lastname: Akiyama
authors.forename: Y
authors.initials: Y
authors.lastname: Nishi
authors.forename: H
authors.initials: H
authors.lastname: Sasai
mesh_terms: D000818:Animals; D002678:Chimera; D002872:Chromosome Deletion; D004622:Embryo, Mammalian / Q000166:cytology; D005260:Female; D018095:Germ-Line Mutation; D007041:Hypoxanthine Phosphoribosyltransferase / Q000235:genetics*; D008297:Male; D051379:Mice; D008817:Mice, Mutant Strains; D013234:Stem Cells / Q000528:radiation effects*; D014960:X Chromosome
chemical_list: D007041:Hypoxanthine Phosphoribosyltransferase