Identification of several genes differentially expressed during progression of chronic myelogenous leukemia.
The Bcr-Abl fusion protein plays a crucial role in the initiation and maintenance of chronic myelogenous leukemia (CML). However, additional events are necessary for the transition from the chronic phase to the terminal phase of the disease. To identify genes involved in the disease progression, we constructed a subtractive library from enriched K562 cell mRNA. We obtained 1084 cDNA clones. After a specific hybridization of these clones with a cDNA probe from either chronic phase or K562 cells, 43 clones which present a differential hybridization level have been selected. Among them, several clones corresponded to ribosomal protein genes showing an increased transcription level during the blast crisis. We observed variations in the expression of a cellular adhesion molecule, a laminin-binding protein. An increased transcription level of the MAZ gene has been shown in the terminal phase of the disease. This gene encodes a protein that regulates the transcription of myc.
Dahéron
L
L
Laboratoire de Génétique Cellulaire et Moléculaire, CHU de Poitiers, France.
Salmeron
S
S
Patri
S
S
Brizard
A
A
Guilhot
F
F
Chomel
J C
JC
Kitzis
A
A
D001752:Blast Crisis; D002352:Carrier Proteins / Q000096:biosynthesis; D015815:Cell Adhesion Molecules / Q000096:biosynthesis; D017931:DNA Primers; D004268:DNA-Binding Proteins; D018450:Disease Progression; D015972:Gene Expression Regulation, Neoplastic; D015723:Gene Library; D006801:Humans; D007797:Laminin / Q000378:metabolism; D015464:Leukemia, Myelogenous, Chronic, BCR-ABL Positive / Q000097:blood / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D007958:Leukocyte Count; D016133:Polymerase Chain Reaction; D016271:Proto-Oncogene Proteins c-myc / Q000096:biosynthesis; D012333:RNA, Messenger / Q000096:biosynthesis; D012269:Ribosomal Proteins / Q000096:biosynthesis; D014157:Transcription Factors / Q000096:biosynthesis; D014158:Transcription, Genetic; D014407:Tumor Cells, Cultured
D002352:Carrier Proteins; D015815:Cell Adhesion Molecules; D017931:DNA Primers; D004268:DNA-Binding Proteins; D007797:Laminin; D016271:Proto-Oncogene Proteins c-myc; D012333:RNA, Messenger; D012269:Ribosomal Proteins; D014157:Transcription Factors; C076035:c-MYC-associated zinc finger protein