Induction of (pre) gastrulation and/or (pre) neurulation by subgerminal ooplasm and Rauber's sickle in cultured anti-sickle regions of avian unincubated blastoderms.
Rauber's sickle fragments from unincubated quail blastoderms, associated or not with chicken central subgerminal ooplasm, were placed on the deep side of the upper layer (UL) of the isolated anti-sickle region of unincubated chicken blastoderms and cultured in vitro. When only a Rauber's sickle fragment was placed, we observed always a pronounced thickening of the UL (pregastrulation) in the immediate neighbourhood. Some times a primitive streak (PS) developed. When the Rauber's sickle fragment was "sandwiched" between the UL and a central subgerminal ooplasmic mass [containing the nucleus of Pander (1817)], always a (pre)neural plate accompanied by endophyll developed, not or well associated with a primitive streak. In the latter case a complete miniature embryo developed. De novo formation of endophyll was observed. As it contained quail nuclei it was derived from Rauber's sickle cells which colonized the subgerminal ooplasm. Our experiments indicate that the uncommitted upper layer (UL) of the anti-sickle of unincubated blastoderms constitutes an excellent reactor tissue for inductions. The thickening induced in the UL of the anti-sickle region by Rauber's sickle initiates (pre)gastrulation and the thickening induced by endophyll initiates (pre)neurulation. In the beginning, pregastrulation and preneurulation seem to be independent phenomena. It is only later, when both become correctly linked at the right place and time, that a normal embryo will develop.
Callebaut
M
M
Laboratory of Human Anatomy and Embryology, RUCA, UA, Antwerpen, Belgium.
Van Nueten
E
E
Harrisson
F
F
Van Nassauw
L
L
Schrevens
A
A
D000818:Animals; D001756:Blastoderm / Q000502:physiology*; D002478:Cells, Cultured; D002642:Chick Embryo; D002645:Chickens; D003470:Culture Media; D003593:Cytoplasm / Q000502:physiology*; D005775:Gastrula / Q000502:physiology*; D009420:Nervous System / Q000196:embryology*; D011784:Quail
D003470:Culture Media