title: Meiotic products of a Klinefelter 47,XXY male as determined by sperm fluorescence in-situ hybridization analysis.
abstract: The meiotic segregation of 24 spermatozoa obtained from a 47,XXY male is described. Three-colour fluorescence in-situ hybridization with probes for chromosomes X, Y and 18 was used. Five spermatozoa carried an X chromosome, seven carried a Y, six had an XY gonosomal complement, five were missing the sex chromosome and one spermatozoon was presumably diploid with an XX/1818 complement. Our results support the hypothesis that XXY cells are able to complete meiosis. In this patient, the percentage of spermatozoa with an abnormal number of sex chromosomes increased from 1/6 (17%) among spermatozoa with normal morphology to 11/18 (61%) in spermatozoa with abnormal morphology.
authors.affiliation: Department of Human Genetics, Allegheny University of the Health Sciences, Allegheny General Hospital, Pittsburgh, PA 15212, USA.
authors.forename: A M
authors.initials: AM
authors.lastname: Estop
authors.forename: S
authors.initials: S
authors.lastname: Munné
authors.forename: K M
authors.initials: KM
authors.lastname: Cieply
authors.forename: K K
authors.initials: KK
authors.lastname: Vandermark
authors.forename: A N
authors.initials: AN
authors.lastname: Lamb
authors.forename: H
authors.initials: H
authors.lastname: Fisch
mesh_terms: D002887:Chromosomes, Human, Pair 18; D015342:DNA Probes; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D007621:Karyotyping; D007713:Klinefelter Syndrome / Q000235:genetics*; D008297:Male; D008540:Meiosis / Q000235:genetics*; D013094:Spermatozoa / Q000473:pathology*; D014960:X Chromosome; D014998:Y Chromosome
chemical_list: D015342:DNA Probes