A method for fluorescence in situ hybridization against synaptonemal complex-associated chromatin of plant meiocytes.
An improved method of preparing two-dimensional surface spreads of plant synaptonemal complexes (SCs) associated with fluorescent in situ hybridization is described. This technique produces clear preparations of SCs and, in addition, consistently reveals the organization and location of different repetitive DNA sequences in plant meiotic prophase chromosomes.
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Departamento de Genética, Facultad de Biología, Universidad Complutense, Madrid, Spain.
Santos
J L
JL
D002843:Chromatin / Q000235:genetics / Q000648:ultrastructure*; D002874:Chromosome Mapping; D018744:DNA, Plant / Q000737:chemistry*; D002523:Edible Grain / Q000235:genetics*; D017404:In Situ Hybridization, Fluorescence / Q000379:methods; D008540:Meiosis; D006109:Poaceae / Q000235:genetics*; D012091:Repetitive Sequences, Nucleic Acid; D013573:Synaptonemal Complex / Q000235:genetics*
D002843:Chromatin; D018744:DNA, Plant