Cloning and characterization of the 5'-flanking region of the human cardiotrophin-1 gene.
To enable the analysis of the regulation of the human cardiotrophin-1 gene expression, the 5'-flanking region of the human cardiotrophin-1 gene was cloned and sequenced. Data bank search revealed several cis- active DNA elements (SP1, CREB, C/EBP, AP1 and AP-2 like and GATA) in the proximal 1.1 kb region. Six nested 5-'terminal deletion mutants from -1091/+39 to -218/+39 were fused to a luciferase reportergene and proved to be functionally active after transfection into COS-7 cells.
Erdmann
J
J
Department of Internal Medicine/Cardiology, Virchow Klinikum, Humboldt University, Berlin, Germany.
Hassfeld
S
S
Kallisch
H
H
Fleck
E
E
Regitz-Zagrosek
V
V
D000818:Animals; D019677:Artificial Gene Fusion; D001483:Base Sequence; D019556:COS Cells; D003001:Cloning, Molecular; D016207:Cytokines / Q000235:genetics* / Q000378:metabolism; D004247:DNA / Q000235:genetics; D017931:DNA Primers / Q000235:genetics; D005786:Gene Expression Regulation; D017930:Genes, Reporter; D006801:Humans; D008156:Luciferases / Q000235:genetics; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D011401:Promoter Regions, Genetic; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000378:metabolism; D017384:Sequence Deletion; D014162:Transfection
D016207:Cytokines; D017931:DNA Primers; D011993:Recombinant Fusion Proteins; D004247:DNA; C092073:cardiotrophin 1; D008156:Luciferases