Accelerated recombinant protein purification process development automated, robotics-based integration of chromatographic purification and analysis.
Recovery of recombinant proteins from endogenous, host molecules can be an experimentally intensive and time-consuming task. Often the time to analyze material during development of recovery procedures is the rate-limiting step. Nowadays, modern techniques and equipment are being specifically engineered to make this effort much more efficient. We present a case study to illustrate how a new, automation tool, designed for easy, systematic methods development, can be used for very rapid process and analytical optimization. This tool uses robotics to integrate process development with rapid LC-based analysis requiring no user intervention. The methods and procedures described can be generalized to any recombinant protein recovery campaign.
Londo
T
T
PerSeptive Biosystems, Inc., Framingham, MA 01701, USA.
Lynch
P
P
Kehoe
T
T
Meys
M
M
Gordon
N
N
D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004926:Escherichia coli / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D007074:Immunoglobulin G; D011994:Recombinant Proteins / Q000302:isolation & purification*; D012371:Robotics; D013205:Staphylococcal Protein A / Q000302:isolation & purification
D007074:Immunoglobulin G; D011994:Recombinant Proteins; D013205:Staphylococcal Protein A