Determination of methamphetamine and related compounds by capillary electrophoresis with UV and laser-induced fluorescence detection.
Methods for the simultaneous determination of methamphetamine (MP) and its related compounds (MPs) using capillary electrophoresis (CE) with UV absorbance and laser-induced fluorescence (LIF) detection are described. In UV detection, MPs were applied to CE without any derivatization procedure and detected at 210 nm for a rapid and simple analysis. Capillary zone electrophoresis (CZE) and electrokinetic capillary electrophoresis (MEKC) were used. MP, amphetamine (AP), 1-phenylethylamine (1-PA as an I.S.), 2-phenylethylamine (2-PA), 4-hydroxymethamphetamine (4-HMP) and 4-hydroxyamphetamine (4-HAP) were separated within 15 min by both CZE and MEKC. Detection limits of MPs were in the range 48-72 fmol/injection for CZE and 85-191 fmol/injection for MEKC. MEKC was successfully applied to the determination of MPs in urine. For a highly sensitive analysis, LIF detection was also examined using 4-fluoro-7-nitro-2,1,3-benzoxadiazole (NBD-F) as a fluorescent derivatization reagent. By the method, in which MPs derivatives were separated within 45 min by MEKC, 22-40 amol/injection of primary amines (AP, 4-HAP and 2-PA) and 690 amol/injection of MP and 300 amol/injection of 4-HMP were detected. The concentration of MP and AP in 50 microliters urine from MP addicts were successfully determined. A comparison of the characteristics for both UV and LIF detections was also discussed.
Kuroda
N
N
School of Pharmaceutical Sciences, Nagasaki University, Japan.
Nomura
R
R
al-Dirbashi
O
O
Akiyama
S
S
Nakashima
K
K
D009327:4-Chloro-7-nitrobenzofurazan / Q000031:analogs & derivatives; D000661:Amphetamine / Q000652:urine; D019075:Electrophoresis, Capillary / Q000379:methods*; D005456:Fluorescent Dyes; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007834:Lasers; D008694:Methamphetamine / Q000031:analogs & derivatives / Q000652:urine*; D010627:Phenethylamines / Q000652:urine; D013050:Spectrometry, Fluorescence; D013056:Spectrophotometry, Ultraviolet; D010136:p-Hydroxyamphetamine / Q000652:urine
D005456:Fluorescent Dyes; D010627:Phenethylamines; C032930:7-fluoro-4-nitrobenzo-2-oxa-1,3-diazole; D008694:Methamphetamine; C005877:pholedrine; D000661:Amphetamine; D009327:4-Chloro-7-nitrobenzofurazan; D010136:p-Hydroxyamphetamine