Expression in Pseudomonas aeruginosa of an erythromycin-resistance determinant that encodes the mphA gene for macrolide 2'-phosphotransferase I from Escherichia coli.
We studied the expression in Pseudomonas aeruginosa of an erythromycin-resistance (EMr) determinant that included the mphA gene for macrolide 2'-phosphotransferase I and originated in Escherichia coli. A recombinant plasmid, pTZ3609, that consisted of the EMr determinant and a broad-host-range vector RSF1010, endowed P. aeruginosa with high-level resistance to erythromycin. Furthermore, the EMr determinant on a self-transferable plasmid, RP1, was transferred from E. coli to P. aeruginosa by conjugation.
Noguchi
N
N
Department of Microbiology, School of Pharmacy, Tokyo University of Pharmacy and Life Science, Hachioji, Japan.
Katayama
J
J
D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D004352:Drug Resistance, Microbial / Q000235:genetics*; D004917:Erythromycin / Q000494:pharmacology*; D004926:Escherichia coli / Q000201:enzymology*; D008826:Microbial Sensitivity Tests; D017853:Phosphotransferases (Alcohol Group Acceptor) / Q000235:genetics*; D010957:Plasmids; D011550:Pseudomonas aeruginosa / Q000235:genetics*
D000900:Anti-Bacterial Agents; D004917:Erythromycin; D017853:Phosphotransferases (Alcohol Group Acceptor); C061369:macrolide 2'-kinase