title: Isolation and characterization of full and truncated forms of human breast carcinoma protein BA46 from human milk fat globule membranes.
abstract: We have isolated and characterized two proteins of 50 and 30 kDa from human milk fat globule membranes of healthy donors. N-terminal and internal sequencing revealed that the 50-kDa protein is the full-length human breast carcinoma protein BA46 that is highly expressed in human breast tumors. The 30-kDa protein is a truncated form of protein BA46 which consists of the C-terminal factor V/VIII-like domain of BA46 and which appears to anchor BA46 to the milk fat globule membrane. Defective release of the epidermal growth factor domain containing a surface RGD motif may be related to involvement of BA46 in breast cancer.
authors.affiliation: Institut für Molekulare Biotechnologie, Jena, Germany.
authors.forename: M G
authors.initials: MG
authors.lastname: Giuffrida
authors.forename: M
authors.initials: M
authors.lastname: Cavaletto
authors.forename: C
authors.initials: C
authors.lastname: Giunta
authors.forename: A
authors.initials: A
authors.lastname: Conti
authors.forename: J
authors.initials: J
authors.lastname: Godovac-Zimmermann
mesh_terms: D000595:Amino Acid Sequence; D000954:Antigens, Surface / Q000737:chemistry / Q000302:isolation & purification*; D001943:Breast Neoplasms / Q000737:chemistry*; D004591:Electrophoresis, Polyacrylamide Gel; D005260:Female; D006801:Humans; D008055:Lipids / Q000737:chemistry*; D008894:Milk Proteins / Q000737:chemistry / Q000302:isolation & purification*; D008895:Milk, Human / Q000737:chemistry*; D008969:Molecular Sequence Data
chemical_list: D000954:Antigens, Surface; D008055:Lipids; C098942:MFGE8 protein, human; D008894:Milk Proteins