Obese gene expression in porcine adipose tissue is reduced by food deprivation but not by maintenance or submaintenance intake.
The relationship between obese gene expression and energy intake was determined in pigs of various body weights. With ad libitum consumption, expression increased (P < 0.001) with body weight from 55 to 163 kg. Obese mRNA relative abundance was correlated with fat mass (r = 0.74, P < 0.0001) and percentage of fat (r = 0.72, P < 0. 0001). Obese expression was also evaluated at 159 kg (initial weight) and ad libitum, maintenance or 23% of maintenance intake for 28 d. Obese mRNA was independent of treatment (P > 0.78) despite considerable weight differences. Obese mRNA abundance was then compared at 136 kg (initial weight) and ad libitum or maintenance intake for 3 or 28 d. Abundance was not influenced by either duration of treatment or intake, despite a small increase (P < 0.01) in serum nonesterified fatty acids (NEFA) and a reduction (P < 0.02) in insulin attributable to maintenance intake. Finally, mRNA abundance was determined at 60 and 136 kg and conditions of food deprivation or ad libitum intake for 3 d. Food deprivation reduced (P < 0.01) serum insulin and increased (4- to 5-fold) NEFA concentrations. Obese mRNA abundance was greater (P < 0.01) in the heavier pigs and was reduced (P < 0.01) by food deprivation. We conclude that obese mRNA abundance in pigs correlates with fat mass and percentage of body fat under conditions of ad libitum intake. Furthermore, obese mRNA abundance is reduced by food deprivation, whereas lesser degrees of intake restriction do not change obese mRNA abundance, even when accompanied by appreciable weight loss.
Spurlock
M E
ME
Swine Research Group, Purina Mills, St. Louis, MO 63144, USA.
Frank
G R
GR
Cornelius
S G
SG
Ji
S
S
Willis
G M
GM
Bidwell
C A
CA
D000273:Adipose Tissue / Q000502:physiology*; D000818:Animals; D001823:Body Composition / Q000502:physiology; D001835:Body Weight / Q000502:physiology; D002149:Energy Intake; D005230:Fatty Acids, Nonesterified / Q000097:blood; D005508:Food Deprivation / Q000502:physiology*; D015870:Gene Expression / Q000502:physiology*; D007328:Insulin / Q000097:blood; D008297:Male; D009765:Obesity / Q000097:blood / Q000235:genetics*; D012333:RNA, Messenger / Q000378:metabolism; D013552:Swine
D005230:Fatty Acids, Nonesterified; D007328:Insulin; D012333:RNA, Messenger