DNA fingerprinting of Riemerella anatipestifer.
Seventeen restriction endonucleases were evaluated for use in DNA fingerprinting of Riemerella anatipestifer. Digestion of chromosomal DNA with either HinfI or DdeI restriction endonuclease, followed by submarine electrophoresis in agarose and staining with ethidium bromide, resulted in DNA fingerprint profiles that could be easily resolved. HinfI produced readable fingerprint patterns in the 2.7-20-kb range and was used to distinguish DNA fingerprint profiles among 89 strains of R. anatipestifer representing isolates from various avian species in the United States, the United Kingdom, Australia, Canada, Germany, and Israel. A total of 52 distinct DNA fingerprint profiles were found.
Rimler
R B
RB
Avian and Swine Respiratory Diseases Research Unit, National Animal Disease Center, Ames, IA 50010, USA.
Nordholm
G E
GE
D000818:Animals; D001315:Australia; D001717:Birds / Q000382:microbiology*; D002170:Canada; D002876:Chromosomes, Bacterial; D016172:DNA Fingerprinting; D004269:DNA, Bacterial / Q000737:chemistry; D015252:Deoxyribonucleases, Type II Site-Specific; D005858:Germany; D016946:Gram-Negative Aerobic Rods and Cocci / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D007557:Israel; D011200:Poultry / Q000382:microbiology*; D015183:Restriction Mapping; D012703:Serotyping; D006113:United Kingdom; D014481:United States
D004269:DNA, Bacterial; C027908:endodeoxyribonuclease DdeI; D015252:Deoxyribonucleases, Type II Site-Specific; C032229:GANTC-specific type II deoxyribonucleases