title: Effect of DNA methylation on potential transcriptional activity in different tissues and organs of Vicia faba ssp. minor.
abstract: Routine protocol was used to isolate DNA (average size 50000 Kbp) from old/young leaves, cotyledons, apical buds and different zones of roots from 4 and 7 days-old Vicia faba seedlings as well as from mature plants. The level of m5dC, as determined by HPLC, varies in the tested material from 22.9% to 31% and could be arranged in the following order: first leaves of seedlings<root<meristematic buds approximately = differentiated zones of root<young leaves<old leaves = cotyledons. This observation suggests that there is a relation between the level of DNA methylation and the processes of differentiation and aging of leaves. DNase I digestion of chromatin of interphase nuclei from different organs of Vicia faba (in situ nick-translation, cytophotometry) indicates that the increase in DNA methylation reduces potential transcriptional activity.
authors.affiliation: Department of Plant Cytology and Cytochemistry, University of Lódź, Poland.
authors.forename: J
authors.initials: J
authors.lastname: Kaźmierczak
mesh_terms: D002454:Cell Differentiation / Q000502:physiology; D002467:Cell Nucleus / Q000378:metabolism; D002843:Chromatin / Q000378:metabolism; D019175:DNA Methylation; D004254:DNA Nucleotidyltransferases / Q000378:metabolism; D018744:DNA, Plant / Q000378:metabolism; D003841:Deoxycytidine / Q000031:analogs & derivatives / Q000032:analysis; D003850:Deoxyribonuclease I / Q000378:metabolism; D003853:Deoxyribonucleosides / Q000032:analysis; D007887:Fabaceae / Q000502:physiology*; D010946:Plants, Medicinal; D013997:Time Factors; D014158:Transcription, Genetic / Q000235:genetics*
chemical_list: D002843:Chromatin; D018744:DNA, Plant; D003853:Deoxyribonucleosides; D003841:Deoxycytidine; C016569:5-methyldeoxycytidine; D004254:DNA Nucleotidyltransferases; D003850:Deoxyribonuclease I