Reduction of 13-hydroperoxy-9,11-octadecadienoic acid by dopamine-melanin.
The effect of dopamine-melanin (DA-melanin), synthetic model of neuromelanin, on the stability of 13-hydroperoxyoctadecadienoic acid (13-HPODE) has been investigated using a reverse-phase high-performance liquid chromatography (HPLC) with UV detection. It was found that DA-melanin effectively accelerated the decomposition of 13-HPODE, both in the absence and in the presence of ferrous ions. The disappearance of 13-HPODE was accompanied by the formation of 13-hydroxyoctadecadienoic acid (13-HODE). The results obtained indicate that DA-melanin is able to reduce linoleic acid hydroperoxide to its stable hydroxyl derivative. The fatty acid hydroperoxide-reducing ability of DA-melanin appears to play an important role in the antioxidative activity of neuromelanin in the process of lipid peroxidation.
Stepień
K
K
Department of Biochemistry and Biophysics, Faculty of Pharmacy, Silesian Academy of Medicine, Sosnowiec, Poland.
Zajdel
A
A
Swierczek
G
G
Wilczok
A
A
Wilczok
T
T
D000975:Antioxidants / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid; D008041:Linoleic Acids / Q000032:analysis / Q000737:chemistry*; D008054:Lipid Peroxides / Q000737:chemistry*; D008543:Melanins / Q000737:chemistry*; D008956:Models, Chemical; D010084:Oxidation-Reduction
D000975:Antioxidants; D008041:Linoleic Acids; D008054:Lipid Peroxides; D008543:Melanins; C081789:dopamine melanin; C014121:neuromelanin; C038649:13-hydroperoxy-9,11-octadecadienoic acid; C024348:13-hydroxy-9,11-octadecadienoic acid