Molecular cloning and functional expression of a rabbit renal organic cation transporter.
A cDNA encoding an organic cation transporter (rbOCT1) was isolated from rabbit kidney. The cDNA encodes a 554 amino acid protein that is highly homologous to other mammalian organic cation transporters. rbOCT1 mediated 3H-1-methyl-4-phenylpyridinium (3H-MPP+) transport in Xenopus laevis oocytes was saturable, sensitive to membrane potential, and inhibited by various organic cations. rbOCT1 mRNA transcripts are expressed in the kidney, liver, and intestine.
Terashita
S
S
Department of Biopharmaceutical Sciences, University of California, San Francisco 94143, USA.
Dresser
M J
MJ
Zhang
L
L
Gray
A T
AT
Yost
S C
SC
Giacomini
K M
KM
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D015152:Blotting, Northern; D002352:Carrier Proteins / Q000235:genetics*; D003001:Cloning, Molecular; D018076:DNA, Complementary; D007668:Kidney / Q000378:metabolism*; D008297:Male; D008565:Membrane Proteins / Q000235:genetics*; D008969:Molecular Sequence Data; D027702:Organic Cation Transporter 1; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000235:genetics; D011817:Rabbits; D014982:Xenopus laevis
D002352:Carrier Proteins; D018076:DNA, Complementary; D008565:Membrane Proteins; D027702:Organic Cation Transporter 1; D012333:RNA, Messenger