Genetic and alkaloid analysis of Papaver species and their F1 hybrid by RAPD, HPLC and ELISA.
Total DNA was extracted from the leaves of a F1 hybrid and its parents, Papaver bracteatum Lindle and P. pseudo-orientale Medw. Analysis of random-amplified polymorphic DNA (RAPD) using ten arbitrary oligonucleotide 10-mers, showed that F1 hybrid was confirmed to be genetically intermediate of both parental plants compared with the genetic distance between F1 hybrid and individual parents. Furthermore, the comparison of the band patterns between a F1 hybrid, P. bracteatum and P. pseudo-orientale clearly showed that part of the bands of both parents were induced into a F1 hybrid. The content of thebaine was determined by HPLC and ELISA used anti-thebaine monoclonal antibody.
Shoyama
Y
Y
Faculty of Pharmaceutical Sciences, Kyushu University, Fukuoka, Japan.
Kawachi
F
F
Tanaka
H
H
Nakai
R
R
Shibata
T
T
Nishi
K
K
D000470:Alkaloids / Q000032:analysis*; D015415:Biomarkers / Q000032:analysis; D002851:Chromatography, High Pressure Liquid; D002875:Chromosomes / Q000235:genetics; D017931:DNA Primers / Q000737:chemistry; D018744:DNA, Plant / Q000032:analysis*; D004797:Enzyme-Linked Immunosorbent Assay; D009294:Narcotics / Q000737:chemistry; D010207:Papaver / Q000737:chemistry* / Q000235:genetics; D010946:Plants, Medicinal; D019105:Random Amplified Polymorphic DNA Technique; D013045:Species Specificity; D013797:Thebaine / Q000737:chemistry
D000470:Alkaloids; D015415:Biomarkers; D017931:DNA Primers; D018744:DNA, Plant; D009294:Narcotics; D013797:Thebaine