NO is not involved in the simvastatin induced cell division and differentiation in PC12 cells.
Simvastatin, a potent 3-hydroxy-3-methylglutaryl coenzyme A (HMG-CoA) reductase inhibitor has been reported to inhibit cell division and induce neurite-like outgrowth in PC12 cells [Sato-Suzuki, I. and Murota, S., Neurosci. Lett., 220 (1996) 21-24]. In the present paper, we examined whether the induced nitric oxide (NO) in the simvastatin-treated PC12 cells is involved in the growth arrest and differentiation as reported in nerve growth factor (NGF) treated PC12 cells. Treatment of PC12 cells with simvastatin caused peripherin formation and enhanced NO production just like NGF-treated PC12 cells. Different from NGF, however, NO synthase inhibitors could not affect the growth arrest and differentiation in simvastatin-treated PC12 cells. In conclusion, NO had nothing to do with cell division and differentiation in simvastatin-treated PC12 cells.
Sano
M
M
Department of Physiological Chemistry, Graduate School, Tokyo Medical and Dental University, Japan.
Sato-Suzuki
I
I
Fujita
H
H
Morita
I
I
Nagao
M
M
Murota
S
S
D000818:Animals; D002454:Cell Differentiation / Q000187:drug effects; D002455:Cell Division / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D004791:Enzyme Inhibitors / Q000494:pharmacology; D019161:Hydroxymethylglutaryl-CoA Reductase Inhibitors / Q000494:pharmacology*; D007381:Intermediate Filament Proteins / Q000032:analysis; D008562:Membrane Glycoproteins; D019331:NG-Nitroarginine Methyl Ester / Q000494:pharmacology; D009419:Nerve Tissue Proteins / Q000032:analysis; D016501:Neurites / Q000737:chemistry / Q000187:drug effects / Q000201:enzymology; D009474:Neurons / Q000166:cytology / Q000187:drug effects* / Q000648:ultrastructure; D009569:Nitric Oxide / Q000502:physiology*; D016716:PC12 Cells; D064531:Peripherins; D051381:Rats; D019821:Simvastatin / Q000494:pharmacology*; D019323:omega-N-Methylarginine / Q000494:pharmacology
D004791:Enzyme Inhibitors; D019161:Hydroxymethylglutaryl-CoA Reductase Inhibitors; D007381:Intermediate Filament Proteins; D008562:Membrane Glycoproteins; D009419:Nerve Tissue Proteins; D064531:Peripherins; D019323:omega-N-Methylarginine; D009569:Nitric Oxide; D019821:Simvastatin; D019331:NG-Nitroarginine Methyl Ester