title: Effects of different perfluorochemicals on dorsal root ganglion cells in vitro.
abstract: BACKGROUND
  Investigation of the effects of different perfluorochemicals (PFC) on cultured dorsal root ganglion (DRG) cells.
  
  
  METHODS
  DRG cell cultures from 9- to 11-day-old chicken embryos were exposed to emulsified perfluorodecalin (PFD; C10F18; 0.5%, 1% and 10%) or perfluorooctylbromide (PFO; C8F17Br; 0.5%, 1% and 10%). The cells were evaluated under phase-contrast optics after 30 h and 120 h for 0.5 and 1% and after 5 h for 10%. To study the integrity of neuronal cells, immunohistochemical labelling for neurofilaments (NF) and tubulin (TUB) was performed.
  
  
  RESULTS
  Concentrations of 0.5% and 1% of PFD or PFO did not change immunohistochemical labelling of DRG cells. Co-cultured macrophages showed a foam cell response, presumably representing ingested PFC. At both concentrations PFD induced a weaker foam cell response than PFO. A concentration of 10% led to the death of DRG cells and macrophages within 5 h.
  
  
  CONCLUSIONS
  PFC caused a dose-dependent damage of neuronal cells. Co-cultured macrophages developed a foam cell response similar to that observed in vivo after prolonged presence of PFC in the vitreous body. These observations indicate that PFD and PFO may not be suitable for long-term vitreous replacement in vitreoretinal surgery. However, the model is limited by several factors: (1) there are physiological differences between DRG cells and retinal ganglion cells; (2) in vivo retinal ganglion cells are protected by the overlying tissues; (3) the PFC used in tissue culture must be emulsified.
authors.affiliation: Department of Ophthalmology, University of Bonn, Germany.
authors.forename: D
authors.initials: D
authors.lastname: Meller
authors.forename: A J
authors.initials: AJ
authors.lastname: Augustin
authors.forename: M
authors.initials: M
authors.lastname: Spitznas
authors.forename: J
authors.initials: J
authors.lastname: Lutz
authors.forename: K
authors.initials: K
authors.lastname: Meller
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D002642:Chick Embryo; D018920:Coculture Techniques; D004305:Dose-Response Relationship, Drug; D004655:Emulsions; D005466:Fluorocarbons / Q000633:toxicity*; D005487:Foam Cells / Q000473:pathology; D005727:Ganglia, Spinal / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D006842:Hydrocarbons, Brominated; D007124:Immunoenzyme Techniques; D008858:Microscopy, Phase-Contrast; D016900:Neurofilament Proteins / Q000378:metabolism; D014404:Tubulin / Q000378:metabolism
chemical_list: D004655:Emulsions; D005466:Fluorocarbons; D006842:Hydrocarbons, Brominated; D016900:Neurofilament Proteins; D014404:Tubulin; C008805:perfluorodecalin; C003072:perflubron