title: The complete genome of the hyperthermophilic bacterium Aquifex aeolicus.
abstract: Aquifex aeolicus was one of the earliest diverging, and is one of the most thermophilic, bacteria known. It can grow on hydrogen, oxygen, carbon dioxide, and mineral salts. The complex metabolic machinery needed for A. aeolicus to function as a chemolithoautotroph (an organism which uses an inorganic carbon source for biosynthesis and an inorganic chemical energy source) is encoded within a genome that is only one-third the size of the E. coli genome. Metabolic flexibility seems to be reduced as a result of the limited genome size. The use of oxygen (albeit at very low concentrations) as an electron acceptor is allowed by the presence of a complex respiratory apparatus. Although this organism grows at 95 degrees C, the extreme thermal limit of the Bacteria, only a few specific indications of thermophily are apparent from the genome. Here we describe the complete genome sequence of 1,551,335 base pairs of this evolutionarily and physiologically interesting organism.
authors.affiliation: Diversa Corporation, San Diego, California 92121, USA.
authors.forename: G
authors.initials: G
authors.lastname: Deckert
authors.forename: P V
authors.initials: PV
authors.lastname: Warren
authors.forename: T
authors.initials: T
authors.lastname: Gaasterland
authors.forename: W G
authors.initials: WG
authors.lastname: Young
authors.forename: A L
authors.initials: AL
authors.lastname: Lenox
authors.forename: D E
authors.initials: DE
authors.lastname: Graham
authors.forename: R
authors.initials: R
authors.lastname: Overbeek
authors.forename: M A
authors.initials: MA
authors.lastname: Snead
authors.forename: M
authors.initials: M
authors.lastname: Keller
authors.forename: M
authors.initials: M
authors.lastname: Aujay
authors.forename: R
authors.initials: R
authors.lastname: Huber
authors.forename: R A
authors.initials: RA
authors.lastname: Feldman
authors.forename: J M
authors.initials: JM
authors.lastname: Short
authors.forename: G J
authors.initials: GJ
authors.lastname: Olsen
authors.forename: R V
authors.initials: RV
authors.lastname: Swanson
mesh_terms: D002874:Chromosome Mapping; D002876:Chromosomes, Bacterial; D002952:Citric Acid Cycle; D004260:DNA Repair; D004269:DNA, Bacterial / Q000096:biosynthesis / Q000235:genetics; D016680:Genome, Bacterial; D016946:Gram-Negative Aerobic Rods and Cocci / Q000235:genetics* / Q000378:metabolism; D008969:Molecular Sequence Data; D018384:Oxidative Stress; D010802:Phylogeny; D014176:Protein Biosynthesis; D013696:Temperature; D014158:Transcription, Genetic
chemical_list: D004269:DNA, Bacterial