A dot immunoblotting assay (dot blot ELISA) for early detection of Newcastle disease antibodies in chickens.
An enzyme-linked immunosorbent assay using nitrocellulose blotting membrane (dot blot ELISA) was developed for the detection of antibodies against Newcastle disease virus (NDV) in chickens. In this method, a nitrocellulose blotting membrane was used as the solid phase carrier. NDV antigens were directly bound onto the nitrocellulose membrane that was set into a dot blot microfiltration apparatus. Efficiency of the assay was evaluated using known positive and negative NDV sera obtained from chickens. The ability of the assay to detect antibodies 2 days earlier than the standard hemagglutination-inhibition (HI) test was demonstrated on sera collected from chickens experimentally infected with NDV, La Sota strain.
Folitse
R
R
University of Minnesota, College of Veterinary Medicine, Department of Veterinary PathoBiology, St. Paul 55108, USA.
Halvorson
D A
DA
Sivanandan
V
V
D000818:Animals; D000914:Antibodies, Viral / Q000097:blood*; D002645:Chickens; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D006385:Hemagglutination Inhibition Tests; D015151:Immunoblotting; D007074:Immunoglobulin G / Q000097:blood; D009521:Newcastle Disease / Q000097:blood / Q000175:diagnosis* / Q000276:immunology; D009522:Newcastle disease virus / Q000276:immunology*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
D000914:Antibodies, Viral; D007074:Immunoglobulin G