Differential expression of proteins during healing of cutaneous wounds in experimental normal and chronic models.
Recent studies have demonstrated growth factors and other cellular proteins as being important in the healing process. In this study, we have investigated the differential expression of proteins in wound tissues of normal and chronic animal models. Proteins were identified by specific antibodies, partial N-terminal amino acid sequence, and molecular weight homology. In normal wound tissues de novo synthesis of a heat shock protein, platelet derived growth factor (PDGF), and fibroblast growth factor (FGF) was induced within 24 h of skin injury. Proteins resembling vascular endothelial growth factor, receptors for PGDF, FGF, and epidermal growth factor, were synthesized. The elevated synthesis declined to a basal level in 7 to 14 days after skin injury which coincided with healing of wounds. These changes occurred only in wound site tissues but not in distal tissues. In contrast, the chronic wounds presented a different picture. The expressions of these proteins were either delayed or inhibited. This suggested the role of these proteins during normal and chronic wound healing. The proteins which were down regulated in chronic wounds may be used in the management of wounds and exploited as targets for therapeutic development.
Shukla
A
A
Pharmacology Division, Central Drug Research Institute, Lucknow, India.
Dubey
M P
MP
Srivastava
R
R
Srivastava
B S
BS
D000818:Animals; D004195:Disease Models, Animal; D006133:Growth Substances / Q000378:metabolism; D006360:Heat-Shock Proteins / Q000378:metabolism; D016867:Immunocompromised Host; D008297:Male; D051379:Mice; D008819:Mice, Nude; D008970:Molecular Weight; D011506:Proteins / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley; D017978:Receptors, Growth Factor / Q000378:metabolism; D012867:Skin / Q000276:immunology / Q000293:injuries* / Q000378:metabolism*; D014945:Wound Healing / Q000276:immunology / Q000502:physiology*
D006133:Growth Substances; D006360:Heat-Shock Proteins; D011506:Proteins; D017978:Receptors, Growth Factor