Organ-specific genotoxicity of the potent rodent bladder carcinogens o-anisidine and p-cresidine.
We used a modification of the alkaline single-cell gel electrophoresis (SCG) (Comet) assay to evaluate the in vivo genotoxicity of two potent rodent bladder carcinogens, o-anisidine and p-cresidine, in mouse liver, lung, kidney, brain, and bone marrow, and in the mucosa of stomach, colon, and bladder. Male CD-1 mice (8 weeks old) were sacrificed 3 and 24 h after oral administration of o-anisidine at 690 mg/kg or p-cresidine at 595 mg/kg. Both chemicals were dissolved in olive oil. Both chemicals yielded statistically significant DNA damage in bladder mucosa 3 and 24 h after treatment. o-Anisidine yielded DNA damage in the colon at 3 h, but not at 24 h. No significant effects were observed in any other organs. Our results suggest the importance of the urinary bladder as a sentinel organ for evaluating chemical genotoxicity in rodents.
Sasaki
Y F
YF
Laboratory of Genotoxicity, Faculty of Chemical and Biological Engineering, Hachinohe National College of Technology, Aomori, Japan. yfsasaki-c@hachinohe-ct.ac.jp
Nishidate
E
E
Su
Y Q
YQ
Matsusaka
N
N
Tsuda
S
S
Susa
N
N
Furukawa
Y
Y
Ueno
S
S
D000814:Aniline Compounds / Q000633:toxicity*; D000818:Animals; D003106:Colon / Q000187:drug effects / Q000378:metabolism; D004249:DNA Damage; D008297:Male; D051379:Mice; D009152:Mutagenicity Tests; D009153:Mutagens / Q000633:toxicity*; D013997:Time Factors; D001743:Urinary Bladder / Q000187:drug effects* / Q000378:metabolism / Q000648:ultrastructure
D000814:Aniline Compounds; D009153:Mutagens; C013453:cresidine; C003898:2-anisidine