Isolation, molecular characterization, and tissue-specific expression of a novel putative G protein-coupled receptor.
We isolated a 40 kDa integral membrane protein (p40) from human erythrocyte ghosts by affinity chromatography, using a C-terminal peptide of stomatin, and obtained partial sequences which enabled us to isolate two full-length cDNAs from human bone marrow and fetal brain cDNA libraries. The cDNA sequences were identical and encoded a novel putative G protein-coupled receptor (399 amino acids). Northern and RNA dot blot analyses demonstrated that the major 4.8 kb-transcript is predominantly expressed in brain. In situ hybridization studies of tissue sections revealed high expression in neurons of the brain and spinal cord, in thymocytes, megakaryocytes, and macrophages.
Mayer
H
H
Institute of Biochemistry, University of Vienna, Austria.
Salzer
U
U
Breuss
J
J
Ziegler
S
S
Marchler-Bauer
A
A
Prohaska
R
R
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D001798:Blood Proteins / Q000096:biosynthesis / Q000737:chemistry / Q000302:isolation & purification; D001854:Bone Marrow Cells / Q000378:metabolism; D001921:Brain / Q000378:metabolism*; D004910:Erythrocyte Membrane / Q000378:metabolism; D005333:Fetus; D019204:GTP-Binding Proteins; D005786:Gene Expression Regulation; D006801:Humans; D008253:Macaca mulatta; D008565:Membrane Proteins; D008969:Molecular Sequence Data; D009928:Organ Specificity; D011956:Receptors, Cell Surface / Q000096:biosynthesis* / Q000737:chemistry; D014158:Transcription, Genetic
D001798:Blood Proteins; D008565:Membrane Proteins; D011956:Receptors, Cell Surface; C036860:STOM protein, human; D019204:GTP-Binding Proteins