A novel long-acting VIP analog in the guinea pig trachea in vitro.
The relaxant effect of a novel VIP analog, [Arg15,20,21Leu17]-VIP-Gly-Lys-Arg-NH2 was compared with that of the original VIP in the same guinea pig trachea precontracted by carbachol in vitro. The VIP analog caused significantly and concentration-dependent relaxation similarly to the original VIP. In contrast to the original VIP, the VIP analog demonstrated a slow onset and offset of action, with more than 90% of its maximum relaxation remaining 6 h after administration. Peptidase inhibition by captopril and phosphoramidon increased the relaxant effect and duration of action for original VIP but not for the VIP analog.
Yoshihara
S
S
First Department of Pediatrics, Dokkyo University School of Medicine, Tochigi, Japan.
Lindén
A
A
Kashimoto
K
K
Watanabe
S
S
D000420:Albuterol / Q000494:pharmacology; D000595:Amino Acid Sequence; D000818:Animals; D002216:Captopril / Q000494:pharmacology; D002217:Carbachol / Q000494:pharmacology; D006168:Guinea Pigs; D008297:Male; D008969:Molecular Sequence Data; D009126:Muscle Relaxation / Q000187:drug effects; D013329:Structure-Activity Relationship; D013997:Time Factors; D014132:Trachea / Q000187:drug effects*; D014660:Vasoactive Intestinal Peptide / Q000031:analogs & derivatives* / Q000737:chemistry / Q000378:metabolism / Q000494:pharmacology*
C111747:Arg(15,20,21)-Leu(17)-VIP-Gly-Lys-Arg-NH2; D014660:Vasoactive Intestinal Peptide; D002217:Carbachol; D002216:Captopril; D000420:Albuterol