Isolation and purification of two novel streptomycete RNase inhibitors, SaI14 and SaI20, and cloning, sequencing, and expression in Escherichia coli of the gene coding for SaI14.
Two new RNase inhibitors, SaI14 (Mr, approximately 14,000) and SaI20 (Mr, approximately 20,000), were isolated and purified from a Streptomyces aureofaciens strain. The gene sai14, coding for SaI14 protein, was cloned and expressed in Escherichia coli. The alignment of the deduced amino acid sequence of SaI14 with that of barstar, the RNase inhibitor from Bacillus amyloliquefaciens, showed significant similarity between them, especially in the region which contains most of the residues involved in barnase-barstar complex formation.
Krajcikova
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Institute of Molecular Biology, Slovak Academy of Sciences, Bratislava, Slovakia. umbidana@savba.savba.sk
Hartley
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RW
Sevcik
J
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D000595:Amino Acid Sequence; D001407:Bacillus / Q000235:genetics; D001426:Bacterial Proteins; D001483:Base Sequence; D002352:Carrier Proteins / Q000235:genetics* / Q000302:isolation & purification* / Q000378:metabolism; D002876:Chromosomes, Bacterial; D003001:Cloning, Molecular; D004269:DNA, Bacterial / Q000032:analysis / Q000235:genetics; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism; D015870:Gene Expression; D005798:Genes, Bacterial; D047908:Intracellular Signaling Peptides and Proteins; D008969:Molecular Sequence Data; D012260:Ribonucleases / Q000037:antagonists & inhibitors; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D013304:Streptomyces aureofaciens / Q000201:enzymology / Q000235:genetics* / Q000378:metabolism*
D001426:Bacterial Proteins; D002352:Carrier Proteins; D004269:DNA, Bacterial; D047908:Intracellular Signaling Peptides and Proteins; C111308:SaI14 protein, Streptomyces aureofaciens; D012260:Ribonucleases