Detailed map of a region commonly amplified at 11q13-->q14 in human breast carcinoma.
Amplification of loci present on band q13 of human chromosome 11 is a feature of a subset of estrogen receptor positive breast carcinomas prone to metastasis. As many as five distinct amplification units have been described on 11q13. They include particularly a genomic area encompassing the GARP gene at 11q13.5-->q14.1. We have reassessed our current knowledge of this region, located telomeric to CCND1 and EMS1, which is amplified in 7-10% of mammary tumors. The loose definition of the driving forces of these amplification events led us to map accurately the boundaries of the amplifiable region, and thus to contribute a physical and transcriptional map of a 3-Mb region of chromosome 11. Four new genes were placed on the regional map, namely CBP2, CLNS1A, UVRAG, and PAK1. We have narrowed the core of the 11q13-->q14 amplicon to a 350-kb area encompassing D11S533, mostly on its telomeric side. The map reported here represents an indispensable step toward sequencing the entire region, and thus toward uncovering gene(s) which play(s) a critical role in breast cancer progression.
Bekri
S
S
Instabilité et Altérations des Génomes, UNSA/CNRS UMR 6549, Nice, France.
Adélaïde
J
J
Merscher
S
S
Grosgeorge
J
J
Caroli-Bosc
F
F
Perucca-Lostanlen
D
D
Kelley
P M
PM
Pébusque
M J
MJ
Theillet
C
C
Birnbaum
D
D
Gaudray
P
P
D001943:Breast Neoplasms / Q000235:genetics*; D002874:Chromosome Mapping; D018244:Chromosomes, Artificial, Yeast; D002880:Chromosomes, Human, Pair 11; D003001:Cloning, Molecular; D004273:DNA, Neoplasm / Q000032:analysis; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans
D004273:DNA, Neoplasm