Structure of genes for cecropin A and an inducible nuclear protein that binds to the promoter region of the genes from the silkworm, Bombyx mori.
Cecropins are a family of antibacterial peptide synthesized in insects as a response to bacterial infection. To study the regulation of the immune genes in insects, two cecropin A genes were cloned and sequenced from the silkworm, Bombyx mori. The two genes, CecA1 and CecA2, encoded identical preprocecropin A, having one intron of 609 bp and 929 bp, respectively. The 5'-upstream regions of the genes contained a NF-kappa B like element and IL-6-RE Type I element. Electrophoretic mobility shift assay revealed that a nuclear protein of fat body which specifically bound to the kappa B-like element was activated by injection of the larvae with peptidoglycan.
Yamano
Y
Y
Department of Biochemistry and Biotechnology, Faculty of Agriculture, Tottori University, Japan.
Matsumoto
M
M
Sasahara
K
K
Sakamoto
E
E
Morishima
I
I
D000595:Amino Acid Sequence; D000818:Animals; D000900:Anti-Bacterial Agents / Q000737:chemistry; D023181:Antimicrobial Cationic Peptides; D001407:Bacillus / Q000276:immunology; D001483:Base Sequence; D015139:Blotting, Southern; D012831:Bombyx / Q000235:genetics* / Q000276:immunology; D004591:Electrophoresis, Polyacrylamide Gel; D005786:Gene Expression Regulation; D007301:Insect Hormones / Q000737:chemistry / Q000235:genetics*; D008969:Molecular Sequence Data; D016328:NF-kappa B / Q000737:chemistry; D009687:Nuclear Proteins / Q000235:genetics*; D010455:Peptides / Q000737:chemistry / Q000235:genetics*; D010457:Peptidoglycan / Q000276:immunology; D011401:Promoter Regions, Genetic; D019947:Receptors, Interleukin-6 / Q000737:chemistry; D017422:Sequence Analysis, DNA
D000900:Anti-Bacterial Agents; D023181:Antimicrobial Cationic Peptides; D007301:Insect Hormones; D016328:NF-kappa B; D009687:Nuclear Proteins; D010455:Peptides; D010457:Peptidoglycan; D019947:Receptors, Interleukin-6; C031161:cecropin A