title: SNAREpins: minimal machinery for membrane fusion.
abstract: Recombinant v- and t-SNARE proteins reconstituted into separate lipid bilayer vesicles assemble into SNAREpins-SNARE complexes linking two membranes. This leads to spontaneous fusion of the docked membranes at physiological temperature. Docked unfused intermediates can accumulate at lower temperatures and can fuse when brought to physiological temperature. A supply of unassembled v- and t-SNAREs is needed for these intermediates to form, but not for the fusion that follows. These data imply that SNAREpins are the minimal machinery for cellular membrane fusion.
authors.affiliation: Cellular Biochemistry and Biophysics Program, Memorial Sloan-Kettering Cancer Center, New York, New York 10021, USA.
authors.forename: T
authors.initials: T
authors.lastname: Weber
authors.forename: B V
authors.initials: BV
authors.lastname: Zemelman
authors.forename: J A
authors.initials: JA
authors.lastname: McNew
authors.forename: B
authors.initials: B
authors.lastname: Westermann
authors.forename: M
authors.initials: M
authors.lastname: Gmachl
authors.forename: F
authors.initials: F
authors.lastname: Parlati
authors.forename: T H
authors.initials: TH
authors.lastname: Söllner
authors.forename: J E
authors.initials: JE
authors.lastname: Rothman
mesh_terms: D001426:Bacterial Proteins / Q000378:metabolism; D002462:Cell Membrane / Q000737:chemistry / Q000502:physiology*; D004269:DNA, Bacterial / Q000302:isolation & purification; D004926:Escherichia coli / Q000235:genetics; D007425:Intracellular Membranes / Q000737:chemistry / Q000378:metabolism; D008051:Lipid Bilayers / Q000737:chemistry / Q000378:metabolism; D008565:Membrane Proteins / Q000235:genetics* / Q000378:metabolism*; D009419:Nerve Tissue Proteins / Q000235:genetics / Q000378:metabolism; D011994:Recombinant Proteins / Q000378:metabolism; D050600:SNARE Proteins; D033921:Vesicular Transport Proteins
chemical_list: D001426:Bacterial Proteins; D004269:DNA, Bacterial; D008051:Lipid Bilayers; D008565:Membrane Proteins; D009419:Nerve Tissue Proteins; D011994:Recombinant Proteins; D050600:SNARE Proteins; D033921:Vesicular Transport Proteins