[Use of the magnitude of the histone-DNA ratio as a criterion for evaluating the functional activity of the cell genome].
Cytophotometry was used to demonstrate, on a variety of experimental models, that changes in the histone/DNA ratio after staining the cell nucleus (Feulgen, DNA) and histones (naphthol yellow S) reflect alterations in the cell functional status. This ratio may thus be used as a criterion for evaluation of cell genome functional activity which is needed in various biological and medical problems.
Shabalkin
I P
IP
Cancer Research Center, Russian Academy of Medical Sciences, Moscow.
D000818:Animals; D001717:Birds / Q000235:genetics*; D001921:Brain / Q000166:cytology; D002467:Cell Nucleus / Q000737:chemistry; D004247:DNA / Q000032:analysis*; D005260:Female; D016678:Genome; D006657:Histones / Q000032:analysis*; D007421:Intestine, Small / Q000166:cytology; D007668:Kidney / Q000166:cytology; D008099:Liver / Q000166:cytology; D008321:Mammary Glands, Animal / Q000166:cytology; D051379:Mice; D051381:Rats
D006657:Histones; D004247:DNA
Ispol'zovanie velichiny otnosheniia giston/DNK v kachestve kriteriia otsenki funktsional'noĭ aktivnosti genoma kletki.