Lack of evidence for GB virus C/hepatitis G virus replication in peripheral blood mononuclear cells.
OBJECTIVE
The recently identified hepatitis G virus (HGV) has been found to be common in patients with various forms of chronic liver disease, particularly chronic hepatitis C. However, replication sites of this new viral agent have not been studied.


METHODS
We searched for the presence of HGV RNA in peripheral blood mononuclear cells and serum samples from nine chronic hepatitis C patients coinfected with hepatitis G virus. The presence of negative viral RNA strands was determined by strand-specific Tth-based assay which was optimized on synthetic template.


RESULTS
All peripheral blood mononuclear cell samples were negative for the presence of the HGV RNA minus strand and only five were positive for the presence of the positive strand, albeit at a low level of 10-10(2) genomic equivalents/10(6) cells.


CONCLUSIONS
These findings imply that hepatitis G virus does not replicate in peripheral blood mononuclear cells, at least in the population of HCV/HGV coinfected patients.
Radkowski
M
M
Institute of Infectious Diseases, Warsaw, Poland.
Wang
L F
LF
Vargas
H
H
Rakela
J
J
Laskus
T
T
D018067:Flaviviridae / Q000302:isolation & purification / Q000502:physiology*; D019698:Hepatitis C, Chronic / Q000097:blood*; D006801:Humans; D007963:Leukocytes, Mononuclear / Q000821:virology*; D016133:Polymerase Chain Reaction / Q000379:methods; D012367:RNA, Viral / Q000302:isolation & purification; D012680:Sensitivity and Specificity; D014158:Transcription, Genetic; D014779:Virus Replication / Q000502:physiology*
D012367:RNA, Viral