title: Sequence-independent method for in vitro generation of nested deletions for sequencing large DNA fragments.
authors.affiliation: Shemyakin and Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Science, Miklukho-Maklaya 16/10, V-437, Moscow, 117871, Russia.
authors.forename: A F
authors.initials: AF
authors.lastname: Fradkov
authors.forename: K A
authors.initials: KA
authors.lastname: Lukyanov
authors.forename: M V
authors.initials: MV
authors.lastname: Matz
authors.forename: L B
authors.initials: LB
authors.lastname: Diatchenko
authors.forename: P D
authors.initials: PD
authors.lastname: Siebert
authors.forename: S A
authors.initials: SA
authors.lastname: Lukyanov
mesh_terms: D017123:Bacteriophage T7 / Q000235:genetics; D001483:Base Sequence; D003001:Cloning, Molecular / Q000379:methods; D004247:DNA / Q000235:genetics* / Q000302:isolation & purification; D017931:DNA Primers / Q000235:genetics; D003850:Deoxyribonuclease I; D016133:Polymerase Chain Reaction / Q000379:methods; D011401:Promoter Regions, Genetic; D017422:Sequence Analysis, DNA / Q000379:methods*; D017384:Sequence Deletion
chemical_list: D017931:DNA Primers; D004247:DNA; D003850:Deoxyribonuclease I