In vitro gene transfer in mammalian cells via a new cationic liposome formulation.
A new cationic liposome formulation of sphingosine (SP) and dioleoylphosphatidylethanolamine (DOPE) was developed as an efficient transfection reagent. This SP/DOPE liposome showed efficient transfection in a wide variety of mammalian cancer cells. No significant cytotoxicity of the SP/DOPE liposome to cells was observed. The tranfection activity was greater than that of a well-reported liposome which was made from a cholesterol derivative 3beta-[N-(N',N'-dimethylaminoethane)-carbamoyl] cholesterol (DC-Chol) and the neutral lipid DOPE. In addition, the SP/DOPE liposome was found to be less toxic to cells than the DC-Chol/DOPE liposome. Stable transfections mediated by SP/ DOPE liposome were also demonstrated. These results suggest that the SP/DOPE liposome may provide a good gene delivery system to be used in the human cancer gene therapy.
Kao
M C
MC
Department of Biochemistry, National Defense Medical Center, P.O. Box 90048-501, Taipei, 100, Taiwan, R.O.C.
Law
S L
SL
Chuang
T C
TC
Lin
Y S
YS
D016475:3T3 Cells; D000818:Animals; D015153:Blotting, Western; D002470:Cell Survival / Q000187:drug effects; D002784:Cholesterol / Q000031:analogs & derivatives / Q000737:chemistry; D005260:Female; D018734:Genes, erbB-2 / Q000235:genetics*; D006801:Humans; D008081:Liposomes / Q000737:chemistry*; D051379:Mice; D009369:Neoplasms / Q000235:genetics*; D010714:Phosphatidylethanolamines / Q000737:chemistry*; D010957:Plasmids; D013110:Sphingosine / Q000737:chemistry*; D014162:Transfection / Q000379:methods*; D014407:Tumor Cells, Cultured; D001616:beta-Galactosidase / Q000235:genetics
D008081:Liposomes; D010714:Phosphatidylethanolamines; C070648:3-(N-(N',N'-dimethylaminoethane)carbamoyl)cholesterol; C020888:1,2-dielaidoylphosphatidylethanolamine; D002784:Cholesterol; D001616:beta-Galactosidase; D013110:Sphingosine