Detection of antitumor promoting activity in Raji cells carrying Epstein-Barr virus genome by immunoblotting analysis.
Extract of Raji cells treated with sodium n-butyrate (1 mM) and a tumor promoter, 12-O-tetradecanoylphorbol-13-acetate (TPA, 40 ng/ml), was analyzed by immunoblotting using ten human sera with different antibody titers against Epstein-Barr virus early antigens. Two human sera reacted with one induced polypeptide of 48 kDa and its induction was inhibited by curcumin (4 micrograms/ml), an antitumor promoter from turmeric. A mouse antiserum against P3HR-1 cells treated with TPA and sodium n-butyrate also detected the 48-kDa polypeptide in Raji cells treated with TPA at concentrations of 2.5 to 80 ng/ml. These results indicate that the immunoblotting analysis can be used in a confirmation test for detection of antitumor promoting activity.
Kondo
A
A
Department of Bioresource Science, Faculty of Agriculture, Kagawa University, Japan.
Morimoto
T
T
Okazaki
K
K
D000818:Animals; D000914:Antibodies, Viral / Q000276:immunology; D000956:Antigens, Viral / Q000276:immunology*; D015153:Blotting, Western; D002087:Butyrates / Q000494:pharmacology*; D020148:Butyric Acid; D002273:Carcinogens / Q000494:pharmacology*; D002461:Cell Line, Transformed; D003474:Curcumin / Q000494:pharmacology*; D004591:Electrophoresis, Polyacrylamide Gel; D016679:Genome, Viral; D004854:Herpesvirus 4, Human / Q000235:genetics / Q000276:immunology*; D006633:Histamine Antagonists / Q000494:pharmacology; D006801:Humans; D015151:Immunoblotting; D051379:Mice; D008856:Microscopy, Fluorescence; D019311:Staurosporine / Q000494:pharmacology; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology*
D000914:Antibodies, Viral; D000956:Antigens, Viral; D002087:Butyrates; D002273:Carcinogens; C033305:Epstein-Barr virus early antigen; D006633:Histamine Antagonists; D020148:Butyric Acid; D019311:Staurosporine; D003474:Curcumin; D013755:Tetradecanoylphorbol Acetate