Identification of a sequence motif that confers SecB dependence on a SecB-independent secretory protein in vivo.
SecB is a cytosolic chaperone which facilitates the transport of a subset of proteins, including membrane proteins such as PhoE and LamB and some periplasmic proteins such as maltose-binding protein, in Escherichia coli. However, not all proteins require SecB for transport, and proteins such as ribose-binding protein are exported efficiently even in SecB-null strains. The characteristics which confer SecB dependence on some proteins but not others have not been defined. To determine the sequence characteristics that are responsible for the SecB requirement, we have inserted a systematic series of short, polymeric sequences into the SecB-independent protein alkaline phosphatase (PhoA). The extent to which these simple sequences convert alkaline phosphatase into a SecB-requiring protein was evaluated in vivo. Using this approach we have examined the roles of the polarity and charge of the sequence, as well as its location within the mature region, in conferring SecB dependence. We find that an insert with as few as 10 residues, of which 3 are basic, confers SecB dependence and that the mutant protein is efficiently exported in the presence of SecB. Remarkably, the basic motifs caused the protein to be translocated in a strict membrane potential-dependent fashion, indicating that the membrane potential is not a barrier to, but rather a requirement for, translocation of the motif. The alkaline phosphatase mutants most sensitive to the loss of SecB are those most sensitive to inhibition of SecA via azide treatment, consistent with the necessity for formation of a preprotein-SecB-SecA complex. Furthermore, the impact of the basic motif depends on location within the mature protein and parallels the accessibility of the location to the secretion apparatus.
Kim
J
J
Department of Molecular and Cell Biology, The University of Connecticut, Storrs 06269, USA.
Kendall
D A
DA
D000251:Adenosine Triphosphatases / Q000187:drug effects / Q000378:metabolism; D000469:Alkaline Phosphatase / Q000235:genetics / Q000378:metabolism; D000595:Amino Acid Sequence; D001386:Azides / Q000494:pharmacology; D001426:Bacterial Proteins / Q000187:drug effects / Q000235:genetics* / Q000378:metabolism* / Q000502:physiology; D002258:Carbonyl Cyanide m-Chlorophenyl Hydrazone; D002462:Cell Membrane / Q000235:genetics / Q000378:metabolism / Q000502:physiology; D003001:Cloning, Molecular; D004269:DNA, Bacterial / Q000032:analysis / Q000235:genetics; D004926:Escherichia coli / Q000235:genetics* / Q000378:metabolism* / Q000502:physiology; D029968:Escherichia coli Proteins; D015870:Gene Expression; D008564:Membrane Potentials / Q000235:genetics / Q000502:physiology; D026901:Membrane Transport Proteins; D008969:Molecular Sequence Data; D016254:Mutagenesis, Insertional; D010957:Plasmids; D000069816:SEC Translocation Channels; D000081416:SecA Proteins; D015398:Signal Transduction; D014178:Translocation, Genetic
D001386:Azides; D001426:Bacterial Proteins; D004269:DNA, Bacterial; D029968:Escherichia coli Proteins; D026901:Membrane Transport Proteins; D000069816:SEC Translocation Channels; C057842:SecB protein, Bacteria; D002258:Carbonyl Cyanide m-Chlorophenyl Hydrazone; D000469:Alkaline Phosphatase; D000251:Adenosine Triphosphatases; D000081416:SecA Proteins