title: The yeast gene YJR025c encodes a 3-hydroxyanthranilic acid dioxygenase and is involved in nicotinic acid biosynthesis.
abstract: We have deleted the yeast gene YJR025c and shown that this leads to an auxotrophy for nicotinic acid. The deduced protein sequence of the gene product is homologous to the human 3-hydroxyanthranilic acid dioxygenase (EC 1.13.11.6) which is part of the kynurenine pathway for the degradation of tryptophan and the biosynthesis of nicotinic acid. In cell-free extracts the 3-hydroxyanthranilic acid dioxygenase activity is proportional to the copy number of the YJR025c gene. As YJR025c encodes the yeast 3-hydroxyanthranilic acid dioxygenase, we have named this gene BNA1 for biosynthesis of nicotinic acid.
authors.affiliation: Institute of Biochemistry and Biophysics, Polish National Academy of Sciences, Warsaw.
authors.forename: R
authors.initials: R
authors.lastname: Kucharczyk
authors.forename: M
authors.initials: M
authors.lastname: Zagulski
authors.forename: J
authors.initials: J
authors.lastname: Rytka
authors.forename: C J
authors.initials: CJ
authors.lastname: Herbert
mesh_terms: D050561:3-Hydroxyanthranilate 3,4-Dioxygenase; D001483:Base Sequence; D049308:Dioxygenases; D017353:Gene Deletion; D005800:Genes, Fungal; D005816:Genetic Complementation Test; D007737:Kynurenine / Q000378:metabolism; D008969:Molecular Sequence Data; D009525:Niacin / Q000096:biosynthesis* / Q000378:metabolism; D010105:Oxygenases / Q000235:genetics* / Q000378:metabolism*; D012441:Saccharomyces cerevisiae / Q000235:genetics* / Q000378:metabolism*; D029701:Saccharomyces cerevisiae Proteins; D014364:Tryptophan / Q000378:metabolism
chemical_list: D029701:Saccharomyces cerevisiae Proteins; D009525:Niacin; D007737:Kynurenine; D014364:Tryptophan; D010105:Oxygenases; D049308:Dioxygenases; D050561:3-Hydroxyanthranilate 3,4-Dioxygenase; C493361:BNA1 protein, S cerevisiae