Lipo-microdialysis: a new microdialysis method for studying the pharmacokinetics of lipophilic substances.
A new microdialysis method (Lipo-MD) using a lipid emulsion as the perfusate instead of Ringer's solution as in conventional microdialysis (MD) was designed. Recovery profiles of the alkylparabens (APBs) dissolved in Ringer' s solution by Lipo-MD were compared with those by MD in vitro. Recovery of APBs in the perfusate in MD decreased with the increasing lipophilicity of APBs, whereas that in Lipo-MD increased. The enhancement of the relative recovery of APBs by Lipo-MD to MD for methyl-, ethyl-, propyl-, and butylparaben were 2.03, 5.24, 77.0, and 390.7, respectively. The utility of Lipo-MD for determination of lipophilic substances to perform pharmacokinetic study was suggested.
Kurosaki
Y
Y
Department of Clinical Pharmaceutical Science, Faculty of Pharmaceutical Sciences, Okayama University, Japan.
Nakamura
S
S
Shiojiri
Y
Y
Kawasaki
H
H
D002851:Chromatography, High Pressure Liquid; D017551:Microdialysis / Q000379:methods*; D010226:Parabens / Q000493:pharmacokinetics*; D013056:Spectrophotometry, Ultraviolet
D010226:Parabens