Simultaneous expression of brain-derived neurotrophic factor and neurotrophin-3 in Cajal-Retzius, subplate and ventricular progenitor cells during early development stages of the rat cerebral cortex.
To identify production sites and action targets of neurotrophins during neurogenesis, we investigated immunoreactivities of neurotrophins and their tyrosine kinase receptors in the cerebral cortex of rat embryos. Two sets of ligand-receptor systems, brain-derived neurotrophic factor/TrkB and neurotrophin-3/TrkC, were expressed simultaneously in Cajal-Retzius, subplate neurons and ventricular multipotent stem cells at embryonic days 13 and 15. Intraventricular administration of brain-derived neurotrophic factor or neurotrophin-3 at embryonic day 16 markedly modulated microtubule-associated protein II and/or Hu protein expression in different ways in the cortical plate cells by embryonic day 20. These observations indicate the involvement of autocrine and/or local paracrine action of brain-derived neurotrophic factor and/or neurotrophin-3 during formation of the cerebral cortex.
Fukumitsu
H
H
Laboratory of Molecular Biology, Gifu Pharmaceutical University, Japan.
Furukawa
Y
Y
Tsusaka
M
M
Kinukawa
H
H
Nitta
A
A
Nomoto
H
H
Mima
T
T
Furukawa
S
S
D000375:Aging / Q000378:metabolism; D000818:Animals; D019208:Brain-Derived Neurotrophic Factor / Q000378:metabolism* / Q000494:pharmacology; D064032:Calbindin 2; D002540:Cerebral Cortex / Q000187:drug effects / Q000196:embryology* / Q000378:metabolism; D004622:Embryo, Mammalian / Q000378:metabolism* / Q000502:physiology; D005314:Embryonic and Fetal Development / Q000502:physiology; D008869:Microtubule-Associated Proteins / Q000378:metabolism; D009414:Nerve Growth Factors / Q000378:metabolism* / Q000494:pharmacology; D009419:Nerve Tissue Proteins / Q000378:metabolism; D020933:Neurotrophin 3; D051381:Rats / Q000196:embryology; D017208:Rats, Wistar; D020794:Receptor Protein-Tyrosine Kinases / Q000378:metabolism; D064030:S100 Calcium Binding Protein G / Q000378:metabolism; D014018:Tissue Distribution
D019208:Brain-Derived Neurotrophic Factor; D064032:Calbindin 2; D008869:Microtubule-Associated Proteins; D009414:Nerve Growth Factors; D009419:Nerve Tissue Proteins; D020933:Neurotrophin 3; D064030:S100 Calcium Binding Protein G; D020794:Receptor Protein-Tyrosine Kinases