title: Exposing guinea pigs to ozone for 1 wk enhances responsiveness of rapidly adapting receptors.
abstract: Acute exposure to ozone causes changes in breathing pattern and lung function which may be caused in part by stimulation of rapidly adapting receptors (RARs). The consequences of repeated daily ozone exposure on RAR responsiveness are unknown, although ozone-induced changes in pulmonary function diminish with repeated exposure. Accordingly, we investigated whether repeated daily ozone exposure diminishes the general responsiveness of RARs. Guinea pigs (n = 30) were exposed to 0.5 parts/million ozone or filtered air (8 h/day for 7 days). The animals were then anesthetized, and RAR impulse activity, dynamic compliance (Cdyn), and lung resistance were recorded at baseline and in response to four stimuli: substance P, methacholine, hyperinflation, and removal of positive end-expiratory pressure. Repeated daily ozone exposure exaggerated RAR responses to substance P, methacholine, and hyperinflation without causing physiologically relevant effects on baseline or substance P- and methacholine-induced changes in Cdyn and lung resistance. Because agonist-evoked changes in RAR activity preceded Cdyn changes, the data suggest that repeated daily ozone exposure enhances RAR responsiveness via a mechanism other than changes in Cdyn.
authors.affiliation: Department of Pediatrics, School of Medicine, University of California at Davis, Davis, California 95616, USA. jpjoad@ucdavis.edu
authors.forename: J P
authors.initials: JP
authors.lastname: Joad
authors.forename: K S
authors.initials: KS
authors.lastname: Kott
authors.forename: A C
authors.initials: AC
authors.lastname: Bonham
mesh_terms: D000818:Animals; D004305:Dose-Response Relationship, Drug; D006168:Guinea Pigs; D008170:Lung Compliance / Q000187:drug effects; D008297:Male; D008465:Mechanoreceptors / Q000187:drug effects*; D016210:Methacholine Chloride / Q000494:pharmacology; D018721:Muscarinic Agonists / Q000494:pharmacology; D010083:Oxidants, Photochemical / Q000633:toxicity*; D010126:Ozone / Q000633:toxicity*; D015656:Respiratory Mechanics / Q000187:drug effects*; D013373:Substance P / Q000494:pharmacology
chemical_list: D018721:Muscarinic Agonists; D010083:Oxidants, Photochemical; D016210:Methacholine Chloride; D013373:Substance P; D010126:Ozone