title: Sequential expression of mRNA for proinflammatory cytokines and interleukin-10 in the rat peripheral nervous system: comparison between immune-mediated demyelination and Wallerian degeneration.
abstract: This study examined the time course of mRNA levels of the proinflammatory cytokines interferon-gamma (IFNgamma), interleukin-1beta (IL1beta), interleukin-12 (IL12; p40 subunit), and the immunosuppressant interleukin-10 (IL10) by semiquantitative reverse transcription polymerase chain reaction (RT-PCR) in rats with actively induced experimental autoimmune neuritis (EAN) and in distal stumps of crushed sciatic nerves undergoing Wallerian degeneration. In EAN IFNgamma- and IL1beta-mRNA peaked at the onset and acute phase of clinical disease. IL12p40-mRNA was upregulated later than IFNgamma-mRNA in the late acute phase from days 15 to 21. IL10-mRNA appeared concomitantly with the proinflammatory cytokines at day 11, but persisted at high levels into the clinical recovery phase. After nerve crush both IL1beta- and IL10-mRNA were rapidly upregulated in the distal stump at day 1 and slowly declined over the next 2 weeks. Significant levels of mRNA for IFNgamma could be found at days 4 and 7, whereas IL12p40-mRNA showed a biphasic induction. We provide evidence for a concomitant induction of pro- and anti-inflammatory cytokines in EAN. Moreover, the rapid upregulation in Wallerian degeneration suggests a more general role of cytokines in the biology of the peripheral nerve.
authors.affiliation: Department of Neurology, Heinrich-Heine-Universität, Düsseldorf, Germany.
authors.forename: C
authors.initials: C
authors.lastname: Gillen
authors.forename: S
authors.initials: S
authors.lastname: Jander
authors.forename: G
authors.initials: G
authors.lastname: Stoll
mesh_terms: D000818:Animals; D001327:Autoimmune Diseases / Q000276:immunology / Q000378:metabolism; D016207:Cytokines / Q000096:biosynthesis / Q000235:genetics*; D003711:Demyelinating Diseases / Q000276:immunology* / Q000503:physiopathology*; D005260:Female; D007249:Inflammation / Q000235:genetics / Q000276:immunology; D016753:Interleukin-10 / Q000235:genetics*; D008297:Male; D009409:Nerve Crush; D009444:Neuritis, Autoimmune, Experimental / Q000276:immunology / Q000378:metabolism; D012333:RNA, Messenger / Q000096:biosynthesis*; D051381:Rats; D011917:Rats, Inbred Lew; D017208:Rats, Wistar; D012584:Sciatic Nerve / Q000502:physiology; D014855:Wallerian Degeneration / Q000276:immunology / Q000378:metabolism / Q000503:physiopathology*
chemical_list: D016207:Cytokines; D012333:RNA, Messenger; D016753:Interleukin-10