Ultrastructural localization of enzymes in biomembranes as revealed by new enzyme cytochemical techniques.
In this mini-review, we describe new enzyme cytochemical methodology combined with cryotechniques, which we have developed in order to reveal the ultrastructural localization of enzymes in biological membranes. This combined approach has been shown to be a valuable tool to study topology, dynamics, and function of enzymes in a more lifelike state.
Takizawa
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Department of Anatomy, Jichi Medical School, Tochigi, Japan.
D000818:Animals; D002462:Cell Membrane / Q000201:enzymology; D004798:Enzymes / Q000032:analysis*; D005614:Freeze Fracturing; D005615:Freezing; D006651:Histocytochemistry / Q000379:methods*; D006801:Humans; D008854:Microscopy, Electron; D008867:Microtomy
D004798:Enzymes