Rapid, sensitive procedure to determine buspirone levels in rat brains using gas chromatography with nitrogen-phosphorus detection.
Reported here is a rapid, sensitive and relatively inexpensive procedure using gas chromatography with nitrogen-phosphorus detection (GC-NPD) to quantify buspirone levels in brains of rats. The analyte was directly extracted from brain homogenate with toluene after basification and then subjected to GC-NPD analysis using a capillary column. The calibration curves were linear over the range of 10 to 320 ng per 2 ml of brain homogenate, with typical r2 values >0.99. The assay was highly reproducible and gave peaks with excellent chromatographic properties.
Lai
C T
CT
Department of Psychiatry (Neurochemical Research Unit), W. Mackenzie Health Sciences Centre, University of Alberta, Edmonton, Canada.
Tanay
V A
VA
Rauw
G A
GA
Bateson
A N
AN
Martin
I L
IL
Baker
G B
GB
D000818:Animals; D014151:Anti-Anxiety Agents / Q000032:analysis*; D001923:Brain Chemistry; D002065:Buspirone / Q000032:analysis*; D002849:Chromatography, Gas / Q000379:methods*; D008297:Male; D009584:Nitrogen; D010758:Phosphorus; D011786:Quality Control; D051381:Rats; D017207:Rats, Sprague-Dawley; D012044:Regression Analysis; D012680:Sensitivity and Specificity
D014151:Anti-Anxiety Agents; D010758:Phosphorus; D009584:Nitrogen; D002065:Buspirone