title: Gene expression of growth and differentiation factors-5, -6, and -7 in developing bovine tooth at the root forming stage.
abstract: Growth and differentiation factors (GDF)-5, -6, and -7 are members of the bone morphogenetic protein (BMP) family. Previous studies suggest their importance in bone development and in tendon/ligament morphogenesis. The cells of the dental attachment apparatus, cementum, periodontal ligament, and alveolar bone proper are derived from the dental follicle proper. In this study, we investigated the expression of GDF-5, -6, and -7 genes in tissues of the bovine incisor tooth germ at the root forming stage. The results demonstrate distinct expression of GDFs in both the dental follicle and the odontoblast layer. While GDF-5 and -6 mRNAs were expressed in both the dental follicle and the odontoblast layer, GDF-7 mRNA expression was detected only in the dental follicle. These results indicate that GDFs, expressed in the bovine tooth germ including the dental follicle, may be potent regulatory molecules in the development of the dental attachment apparatus.
authors.affiliation: Department of Periodontology, Faculty of Dentistry, Tokyo Medical and Dental University, Japan.
authors.forename: Y
authors.initials: Y
authors.lastname: Morotome
authors.forename: M
authors.initials: M
authors.lastname: Goseki-Sone
authors.forename: I
authors.initials: I
authors.lastname: Ishikawa
authors.forename: S
authors.initials: S
authors.lastname: Oida
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D019485:Bone Morphogenetic Proteins; D002417:Cattle; D018507:Gene Expression Regulation, Developmental; D055428:Growth Differentiation Factor 5; D055431:Growth Differentiation Factor 6; D006133:Growth Substances / Q000096:biosynthesis / Q000235:genetics*; D007180:Incisor; D008969:Molecular Sequence Data; D012333:RNA, Messenger / Q000096:biosynthesis; D014083:Tooth Germ / Q000254:growth & development / Q000378:metabolism; D014092:Tooth Root / Q000254:growth & development / Q000378:metabolism*
chemical_list: D019485:Bone Morphogenetic Proteins; D055428:Growth Differentiation Factor 5; D055431:Growth Differentiation Factor 6; D006133:Growth Substances; D012333:RNA, Messenger; C086559:growth differentiation factor 7