title: Long-term alleviation of allodynia-like behaviors by intrathecal implantation of bovine chromaffin cells in rats with spinal cord injury.
abstract: Adrenal chromaffin cells produce analgesic substances, such as catecholamines and enkephalins, and intrathecal (i.t.) implantation of either allografted adrenal tissue or xenogenic chromaffin cells produce antinociception in animals. We evaluated the analgesic effect of bovine chromaffin cells in a model of central pain in which rats exhibit chronic allodynia-like behavior after photochemically induced ischemic spinal cord injury. Bovine chromaffin cells or endothelial cells were injected i.t. onto the lumbar spinal cord and their effects on mechanical and cold allodynia-like behaviors were studied for up to 8 weeks. The chronic allodynia-like behavior was stable for months without signs of remission and i.t. implantation of human endothelial cells did not alleviate the chronic allodynia-like behavior for the entire observation period. In contrast, 2 weeks after i.t. implantation of bovine chromaffin cells, the mechanical allodynia was abolished in the spinally injured rats, and the enhanced response to cold stimuli was significantly reduced. The overall effects were significant up to 8 weeks after i.t. implantation, although the anti-allodynic effect decreased towards the end of the observation period. No signs of side-effects were noted after i.t. implantation. The allodynia-like state was temporarily restored by naloxone (0.5 mg/kg) or phentolamine (0.3 mg/kg) injected intraperitoneally. Immunohistochemical examination revealed that tyrosine hydroxylase (TH)-positive chromaffin cells could be identified adjacent to the spinal cord up to 4 weeks after i.t. implantation, whereas at 8 weeks the TH-positive cells were sparse. It is concluded that bovine chromaffin cells stay viable in rat spinal cord for a considerable period of time after i.t. administration and alleviate chronic allodynia-like behavior in spinally injured rats, possibly through activation of opioid and alpha-adrenoceptors. The present results further document a new therapeutic approach for the treatment of chronic neuropathic pain.
authors.affiliation: Department of Medical Laboratory Sciences and Technology, Karolinska Institute, Huddinge University Hospital, Sweden.
authors.forename: W
authors.initials: W
authors.lastname: Yu
authors.forename: J X
authors.initials: JX
authors.lastname: Hao
authors.forename: X J
authors.initials: XJ
authors.lastname: Xu
authors.forename: J
authors.initials: J
authors.lastname: Saydoff
authors.forename: A
authors.initials: A
authors.lastname: Haegerstrand
authors.forename: T
authors.initials: T
authors.lastname: Hökfelt
authors.forename: Z
authors.initials: Z
authors.lastname: Wiesenfeld-Hallin
mesh_terms: D000818:Animals; D001522:Behavior, Animal; D002417:Cattle; D017690:Cell Transplantation; D019439:Chromaffin Cells / Q000637:transplantation*; D003080:Cold Temperature; D004195:Disease Models, Animal; D004727:Endothelium / Q000166:cytology; D005260:Female; D006930:Hyperalgesia / Q000503:physiopathology* / Q000628:therapy*; D007278:Injections, Spinal; D009270:Naloxone / Q000494:pharmacology; D009292:Narcotic Antagonists / Q000494:pharmacology; D009475:Neurons, Afferent / Q000737:chemistry / Q000187:drug effects / Q000201:enzymology; D010646:Phentolamine / Q000494:pharmacology; D011312:Pressure; D051381:Rats; D017207:Rats, Sprague-Dawley; D011942:Receptors, Adrenergic, alpha / Q000502:physiology; D011957:Receptors, Opioid / Q000502:physiology; D013119:Spinal Cord Injuries / Q000628:therapy*; D013565:Sympatholytics / Q000494:pharmacology; D013623:Tail; D014446:Tyrosine 3-Monooxygenase / Q000032:analysis; D014828:Vocalization, Animal
chemical_list: D009292:Narcotic Antagonists; D011942:Receptors, Adrenergic, alpha; D011957:Receptors, Opioid; D013565:Sympatholytics; D009270:Naloxone; D014446:Tyrosine 3-Monooxygenase; D010646:Phentolamine