title: Fluorescent-protein biosensors: new tools for drug discovery.
abstract: Recent improvements in target discovery and high-throughput screening have increased the pressure at key points along the drug-discovery pipeline. High-content screening was developed to ease the bottlenecks formed at the target-validation and lead-optimization points, and a new generation of reagents that report on specific molecular processes in living cells (fluorescent-protein biosensors) have been important in its development. Creative designs of fluorescent-protein biosensors have emerged and been used to measure the molecular dynamics of macromolecules, metabolites and ions. Recent applications of fluorescent-protein biosensors to biological problems have provided a foundation for their use in biotechnology.
authors.affiliation: Cellomics Inc., Pittsburgh, PA 15238, USA.
authors.forename: K A
authors.initials: KA
authors.lastname: Giuliano
authors.forename: D L
authors.initials: DL
authors.lastname: Taylor
mesh_terms: D015374:Biosensing Techniques; D001709:Biotechnology / Q000639:trends*; D015195:Drug Design; D005456:Fluorescent Dyes; D006801:Humans; D004364:Pharmaceutical Preparations / Q000302:isolation & purification*
chemical_list: D005456:Fluorescent Dyes; D004364:Pharmaceutical Preparations