title: Decreased renal and hepatic blood flow with preeclampsia-like histologic changes was obtained by stimulation of the celiac ganglion with LPS.
abstract: Decreased renal and hepatic blood flow with preeclampsia-like histologic changes was developed by stimulation of the celiac ganglion with lipopolysaccharide (LPS) in pregnant rabbits. The renal and hepatic blood flow were measured after stimulation of the celiac ganglion with 10 microL (group 1), 100 microL (group II) and 300 microL (group III) of LPS (10 mg/mL conc.) and with 100 microL of normal saline (group IV). The bifurcation of the abdominal aorta was also stimulated with 300 microL of LPS (group V). A control experiment was also done in this study (group VI). Histopathological studies of kidney and liver tissue were also performed in this protocol. A significant reduction in renal and hepatic blood flow was observed in pregnant rabbits with the times and dosage dependent on stimulation of the celiac ganglion by LPS. Stimulation of the bifurcation of the abdominal aorta with LPS could not produce any changes in renal and hepatic blood flow. Preeclampsia-like histologic changes of kidney and liver tissue were also observed. These results suggest that exogenous stimulation of the celiac ganglion causes decreased renal and hepatic blood flow, resulting in preeclampsia-like histologic changes of kidney and liver in pregnant rabbits.
authors.affiliation: Department of Obstetrics and Gynecology, Hamamatsu University School of Medicine, Hamamatsu City, Japan.
authors.forename: H M
authors.initials: HM
authors.lastname: Belayet
authors.forename: N
authors.initials: N
authors.lastname: Kanayama
authors.forename: S
authors.initials: S
authors.lastname: Khatun
authors.forename: E
authors.initials: E
authors.lastname: El Maradny
authors.forename: M
authors.initials: M
authors.lastname: Masui
authors.forename: N
authors.initials: N
authors.lastname: Tokunaga
authors.forename: K
authors.initials: K
authors.lastname: Sumimoto
authors.forename: T
authors.initials: T
authors.lastname: Kobayashi
authors.forename: T
authors.initials: T
authors.lastname: Terao
mesh_terms: D000818:Animals; D001012:Aorta, Abdominal / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D005260:Female; D005728:Ganglia, Sympathetic / Q000187:drug effects* / Q000502:physiology; D007668:Kidney / Q000187:drug effects / Q000473:pathology; D017078:Laser-Doppler Flowmetry; D008070:Lipopolysaccharides / Q000633:toxicity*; D008099:Liver / Q000098:blood supply* / Q000187:drug effects / Q000473:pathology; D011225:Pre-Eclampsia / Q000139:chemically induced* / Q000503:physiopathology; D011247:Pregnancy; D011817:Rabbits; D012039:Regional Blood Flow / Q000187:drug effects; D012079:Renal Circulation / Q000187:drug effects* / Q000502:physiology
chemical_list: D008070:Lipopolysaccharides