title: Effect of Pasteurella haemolytica outer membrane proteins on bovine neutrophils.
abstract: The major outer membrane proteins (OMPs) isolated from Pasteurella haemolytica induce alterations of the biological activity of bovine polymorphonuclear leukocytes (PMNs). A dose-dependent reduction of the capacity of adherence to nylon wool in vitro was observed. OMPs also acted as chemotaxins at concentrations between 5 and 20 microg/ml. Concentrations lower than 5 microg/ml did not give considerable results. Preincubation with 5 microg/ml of OMPs led to modifications in the values of the phagocytic index and of intracellular killing, which were found to be diminished with respect to controls.
authors.affiliation: Dipartimento di Patologia, Facoltà di Veterinaria, Università degli Studi di Napoli Federico II, Italy.
authors.forename: G
authors.initials: G
authors.lastname: Iovane
authors.forename: M
authors.initials: M
authors.lastname: Galdiero
authors.forename: M
authors.initials: M
authors.lastname: Vitiello
authors.forename: L
authors.initials: L
authors.lastname: De Martino
mesh_terms: D000818:Animals; D001425:Bacterial Outer Membrane Proteins / Q000276:immunology / Q000302:isolation & purification / Q000502:physiology*; D002417:Cattle; D002448:Cell Adhesion; D002634:Chemotaxis, Leukocyte; D004591:Electrophoresis, Polyacrylamide Gel; D016978:Mannheimia haemolytica / Q000737:chemistry / Q000276:immunology / Q000472:pathogenicity*; D009504:Neutrophils / Q000276:immunology* / Q000502:physiology; D010587:Phagocytosis; D014774:Virulence
chemical_list: D001425:Bacterial Outer Membrane Proteins