Absolute CD4 counts obtained by a three-color flow-cytometric method without the use of a hematology analyzer.
We evaluated the Ortho TRIO-Cytoronabsolute system for determining absolute CD4 counts. The CD4 counts in our blood specimens from 100 individuals ranged from 3 to 1,962; the percent CD4 ranged from 1.3 to 62.2, respectively. The TRIO system was biased toward lower absolute counts than a combination of flow cytometry and hematology but showed no bias in percent CD4 calculations.
Alexander
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Department of Pathology, Summa Health System, Akron, Ohio 44309, USA. talexand@neoucom.edu
D015496:CD4-Positive T-Lymphocytes / Q000473:pathology*; D005434:Flow Cytometry / Q000379:methods*; D006801:Humans; D018655:Lymphocyte Count / Q000379:methods*