Inhibin secretion in the stallion.
To examine the physiological role of inhibin in the stallion, a heterologous radioimmunoassay (RIA) based on a bovine RIA was validated and used to measure immunoreactive (ir)-inhibin concentrations in plasma and testicular homogenates. The bioactivity of equine testicular inhibin was also examined using an assay for suppression of FSH secretion from rat anterior pituitary cells. In addition, to identify the cell responsible for secreting testicular inhibin, the localisation of inhibin in the testis was investigated by an immunohistochemical method using a polyclonal antibody against (Tyr30)-porcine inhibin alpha(1-30) NH2. In the RIA, parallel dose response curves were obtained for the bovine inhibin standard and serial dilutions of stallion plasma and equine testicular homogenates. Parallel FSH inhibition curves were also observed for the bovine inhibin standard and serial dilutions of equine testicular homogenates in the bioassay. The inhibition of FSH secretion from rat pituitary cells by equine testicular homogenates was neutralised by an antiserum against bovine inhibin in vitro. Plasma concentrations of ir-inhibin, testosterone and oestradiol-17beta in stallions decreased abruptly after bilateral gonadectomy and FSH and LH concentrations in the plasma subsequently increased. Therefore, circulating inhibin in the stallion appeared to be largely of testicular origin. The histochemical results showed for the first time that strong immunopositive staining for inhibin occurred in the Leydig cells of the testes. Sertoli cells were also stained by the inhibin antibody but the reaction was weaker than that in Leydig cells. These results indicate clearly that both Leydig and Sertoli cells are potential sources of testicular inhibin in the stallion. A clear increase in plasma ir-inhibin concentrations was observed during the natural breeding season. Similar seasonal changes in the plasma concentrations of testicular steroid hormones and pituitary gonadotrophins occurred throughout the year. In conclusion, the testes appear to be the main source of inhibin, and testicular inhibin is secreted by Leydig and Sertoli cells in stallions. The positive correlations between plasma ir-inhibin and testicular activity during both the breeding and nonbreeding seasons indicate that plasma ir-inhibin is a useful indicator of reproductive activity in the stallion.
Nagata
S
S
Laboratory of Racing Chemistry, Tokyo, Japan.
Miyake
Y I
YI
Nambo
Y
Y
Nagamine
N
N
Watanabe
G
G
Tsunoda
N
N
Taniyama
H
H
Hondo
E
E
Yamada
J
J
Taya
K
K
D000818:Animals; D001947:Breeding; D002417:Cattle; D004306:Dose-Response Relationship, Immunologic; D004958:Estradiol / Q000032:analysis; D005640:Follicle Stimulating Hormone / Q000032:analysis; D006736:Horses / Q000378:metabolism*; D007106:Immune Sera / Q000276:immunology; D007150:Immunohistochemistry; D007265:Inhibins / Q000097:blood / Q000378:metabolism* / Q000502:physiology; D007985:Leydig Cells / Q000378:metabolism; D007986:Luteinizing Hormone / Q000032:analysis; D008297:Male; D011817:Rabbits; D011863:Radioimmunoassay / Q000662:veterinary; D051381:Rats; D012708:Sertoli Cells / Q000378:metabolism; D013737:Testis / Q000166:cytology / Q000378:metabolism*; D013739:Testosterone / Q000032:analysis
D007106:Immune Sera; D013739:Testosterone; D004958:Estradiol; D007265:Inhibins; D007986:Luteinizing Hormone; D005640:Follicle Stimulating Hormone