Isolation and characterization of a cDNA encoding a high mobility group protein HMG-1 from Canavalia gladiata D.C.
A cDNA clone encoding a HMG-1 protein from maturing seeds of Canavalia gladiata was isolated and characterized with respect to its sequence, genomic organization and the expression pattern in seeds. The predicted polypeptide had the characteristic conserved motifs of the HMG-1/2 protein including N-terminal basic region, one HMG-box and polyacidic carboxy terminus. Southern blot analysis suggested that the HMG-1 gene is a single copy gene. Northern blot analysis indicated that the HMG-1 gene was expressed both in maturing and germinated seeds.
Yamamoto
S
S
Department of Biology, Tokyo Metropolitan University, Japan.
Minamikawa
T
T
D000595:Amino Acid Sequence; D001483:Base Sequence; D002352:Carrier Proteins / Q000235:genetics*; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000737:chemistry* / Q000302:isolation & purification*; D018744:DNA, Plant / Q000032:analysis; D007887:Fabaceae / Q000235:genetics*; D018506:Gene Expression Regulation, Plant; D024243:HMGB1 Protein; D006609:High Mobility Group Proteins / Q000235:genetics*; D008969:Molecular Sequence Data; D010940:Plant Proteins / Q000096:biosynthesis / Q000737:chemistry / Q000235:genetics*; D010946:Plants, Medicinal; D012639:Seeds / Q000737:chemistry / Q000235:genetics; D017386:Sequence Homology, Amino Acid
D002352:Carrier Proteins; D018076:DNA, Complementary; D018744:DNA, Plant; D024243:HMGB1 Protein; D006609:High Mobility Group Proteins; D010940:Plant Proteins