Folding and binding activity of the 3'UTRs of Paracentrotus lividus bep messengers.
Bep mRNAs are localized at the animal pole of P. lividus eggs. In the present communication the secondary structures of the 3'UTRs of the bep1, bep3 and bep4 mRNAs are reported. The minimal lengths of these regions required to bind the 54-kDa protein, previously shown to be involved in localization and anchoring of these RNAs, is estimated. Microinjection of the bep3 3'UTR into egg shows that this RNA fragment is also able to become localized to one of the egg poles, as happens for the entire bep3 RNA.
Montana
G
G
Istituto di Biologia dello Sviluppo, C.N.R., Palermo, Italy.
Sbisà
E
E
Romancino
D P
DP
Bonura
A
A
Di Carlo
M
M
D000595:Amino Acid Sequence; D000818:Animals; D007447:Invertebrate Hormones / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D008565:Membrane Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D008845:Microinjections; D008969:Molecular Sequence Data; D014176:Protein Biosynthesis; D017510:Protein Folding; D017433:Protein Structure, Secondary; D012333:RNA, Messenger / Q000235:genetics; D012617:Sea Urchins
D007447:Invertebrate Hormones; D008565:Membrane Proteins; D012333:RNA, Messenger