Preparation and partial structural characterization of alpha1T-glycoprotein from normal human plasma.
alpha1T-glycoprotein (alpha1T) was isolated from normal human plasma in the immunochemically homogeneous state. The partial amino acid sequence and carbohydrate chains of this glycoprotein were determined. To achieve this, the carboxymethylated alpha1T was analyzed by sequencing some of the lysylendoprotease, V8 protease, tryptic, and cyanogen bromide peptides as well as the N-terminal sequence of the protein. A large number of amino acid residues (460 amino acids) was determined by chemical procedure. The peptide sequences were compared with that of other proteins. A high degree of homology was found for proteins of the albumin family. Further, human alpha-albumin, a new member of this protein family, showed an amino acid sequence identical to that of alpha1T indicating that these two proteins are very similar in amino acid sequence and composition. These proteins are closely related to alpha-fetoprotein; however, five carbohydrate chains were found on alpha1T at Asn12, Asn88, Asn362, Asn381, and Asn467 as biantennary complex type chains and the chain on Asn362 possessed a rare consensus sequence of Asn-X-Cys. Thus, alpha1T distinguishes itself by possessing five N-glycans, a finding reported here for the first time for the ALB family.
Araki
T
T
School of Agriculture, Kyushu Tokai, University, Kumamoto, Aso, 869-1404, Japan. taraki@as-1.ktokai-u.ac.jp
Haupt
H
H
Hermentin
P
P
Schwick
H G
HG
Kimura
Y
Y
Schmid
K
K
Torikata
T
T
D000418:Albumins / Q000737:chemistry*; D000595:Amino Acid Sequence; D000818:Animals; D001798:Blood Proteins / Q000737:chemistry*; D002236:Carbohydrate Conformation; D002240:Carbohydrate Sequence; D019143:Evolution, Molecular; D006023:Glycoproteins / Q000737:chemistry*; D006801:Humans; D008969:Molecular Sequence Data; D009844:Oligosaccharides / Q000737:chemistry; D010446:Peptide Fragments / Q000737:chemistry; D017421:Sequence Analysis; D017386:Sequence Homology, Amino Acid; D012709:Serum Albumin; D000075462:Serum Albumin, Human; D000509:alpha-Fetoproteins / Q000737:chemistry
C422455:ALB protein, human; D000418:Albumins; D001798:Blood Proteins; D006023:Glycoproteins; D009844:Oligosaccharides; D010446:Peptide Fragments; D012709:Serum Albumin; D000509:alpha-Fetoproteins; C023442:alpha-albumin; D000075462:Serum Albumin, Human