title: Characterization of a gene from the filamentous fungus Podospora anserina encoding an aspartyl protease induced upon carbon starvation.
abstract: In an attempt to characterize proteases associated with vegetative incompatibility, a Podospora anserina gene (papA) encoding an aspartyl protease (podosporapepsin) was cloned using a heterologous probe. The deduced papA coding region was 1278 nucleotides long, interrupted by a single 71bp intron. The corresponding amino acid sequence presented a high degree of similarity to other aspartyl proteases. Sequence analysis and proteolytic activity measurement suggested that the podosporapepsin could be intracellular rather than secreted. The papA gene was expressed under carbon starvation, but not under nitrogen starvation conditions. Its disruption led to a slight decrease in the growth rate of the mutant strain when bovine serum albumin was the sole carbon source in the medium. Disruption or overexpression of papA gene had no obvious consequence on vegetative incompatibility. Transcription of papA induced by carbon starvation was strongly reduced in the presence of a suppressor of vegetative incompatibility. This result suggests a relationship between adaptation for starvation and vegetative incompatibility.
authors.affiliation: Institut de Biochimie et Génétique Cellulaires, UPR CNRS 9026, Université Victor Segalen Bordeaux II, Bordeaux, France.
authors.forename: M
authors.initials: M
authors.lastname: Paoletti
authors.forename: C
authors.initials: C
authors.lastname: Clavé
authors.forename: J
authors.initials: J
authors.lastname: Bégueret
mesh_terms: D000595:Amino Acid Sequence; D016282:Aspartic Acid Endopeptidases / Q000737:chemistry* / Q000235:genetics; D001483:Base Sequence; D003001:Cloning, Molecular; D003470:Culture Media / Q000737:chemistry; D005656:Fungal Proteins / Q000737:chemistry* / Q000235:genetics; D005658:Fungi / Q000201:enzymology* / Q000378:metabolism; D015971:Gene Expression Regulation, Enzymologic / Q000235:genetics*; D008969:Molecular Sequence Data; D009154:Mutation / Q000235:genetics; D012333:RNA, Messenger / Q000032:analysis; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D014158:Transcription, Genetic / Q000235:genetics
chemical_list: D003470:Culture Media; D005656:Fungal Proteins; D012333:RNA, Messenger; D016282:Aspartic Acid Endopeptidases; C042466:Endothia aspartic proteinase; C111929:podosporapepsin