Ultraviolet exposure influences laser-induced wounds, scars, and hyperpigmentation: a murine study.
Laser therapy is today considered the treatment of choice for vascular skin lesions, which commonly are located on the face and, therefore, frequently are exposed to sunlight. The purpose of this study was to examine whether preoperative and postoperative ultraviolet irradiation influences the development of laser-induced side effects. We laser-treated hairless mice with a copper vapor laser; three different intensities were used at a constant pulse duration. Simulated solar ultraviolet radiation was administered either before the laser treatment (3 consecutive days, daily doses of 2.48 J/cm2) or after the laser treatment (four times weekly in 4 weeks, daily doses of 1.66 J/cm2). Laser-induced wounds, scars, and hyperpigmentation were evaluated by macroscopic, histologic, and biochemical examinations. Preoperative ultraviolet exposure enlarged the laser-induced wounds and the areas with texture change at some of the laser intensities used. However, the most pronounced effect was seen for postoperative ultraviolet-irradiated mice. These mice developed, at some of the laser intensities, a higher incidence of bulging infiltration as well as higher degrees of fibrosis and hyperpigmentation, thus developing a poor cosmetic appearance. Furthermore, ultraviolet irradiation after laser treatment resulted in slowly healing wounds of reduced size, indicating deep, constricted skin damage. We conclude that ultraviolet exposure before and after copper vapor laser treatment increases the risk of inducing side effects from dermatological laser treatment.
Haedersdal
M
M
Department of Dermatology, Bispebjerg Hospital, University of Copenhagen, Denmark.
Bech-Thomsen
N
N
Poulsen
T
T
Wulf
H C
HC
D000818:Animals; D002921:Cicatrix / Q000209:etiology* / Q000378:metabolism / Q000473:pathology; D003286:Contracture / Q000209:etiology; D003300:Copper; D062109:Dermatologic Surgical Procedures; D004954:Esthetics; D005148:Facial Dermatoses / Q000601:surgery; D005260:Female; D005355:Fibrosis; D006901:Hydroxylysine / Q000032:analysis; D006909:Hydroxyproline / Q000032:analysis; D017495:Hyperpigmentation / Q000209:etiology* / Q000378:metabolism / Q000473:pathology; D015994:Incidence; D017075:Laser Coagulation / Q000009:adverse effects*; D051379:Mice; D008812:Mice, Hairless; D008809:Mice, Inbred C3H; D008815:Mice, Inbred Strains; D011392:Proline / Q000032:analysis; D011829:Radiation Dosage; D012307:Risk Factors; D012867:Skin / Q000378:metabolism / Q000473:pathology / Q000528:radiation effects; D012871:Skin Diseases / Q000209:etiology; D017445:Skin Diseases, Vascular / Q000601:surgery; D012880:Skin Pigmentation / Q000528:radiation effects; D013472:Sunlight / Q000009:adverse effects; D014466:Ultraviolet Rays / Q000009:adverse effects*; D014945:Wound Healing / Q000528:radiation effects
D006901:Hydroxylysine; D003300:Copper; D011392:Proline; D006909:Hydroxyproline