title: Determination of the mycotoxin fumonisins in gluten-free diet (corn-based commodities) in the Czech Republic.
abstract: The fumonisins, mycotoxins produced by Fusarium moniliforme, are known to occur worldwide as natural contaminants of corn. They are associated with several animal diseases and are a potential threat to human health. A total of 127 samples of corn-based foods (gluten-free diet) in the Czech Republic were analysed by Ridascreen Fumonisin Fast ELISA methods in years 1995-1996. Eighty eight % of the corn-based foods were found to be positive for fumonisins (FB1, FB2, FB3) and 12% of the examined corn-based foods laid below of a determination limit which was about 9 ng fumonisins/g corn-based foods. The highest fumonisin contamination levels were recorded in extruded corn products containing up to 1,808 micrograms/kg of fumonisins. Levels ranging from < 9 to 1,243 ng/g fumonisins were detected in polenta. Lower levels of fumonisins were found in other commodities, such as corn flour (up to 487 ng/g), corn instant porridge (up to 788 ng/g), and corn pastes (511 ng/g). Intake of fumonisins from several corn-based foods (gluten-free diet) for the population with coeliac disease was estimated. The highest estimate of exposure dose of fumonisins was determined from corn-extruded bread: 3.2 micrograms/person/day (mean of measured values). Daily intake of fumonisins from polenta is expected 2.8 micrograms/person/day (mean). The lower exposure dose of fumonisins we can expect from corn instant porridge, corn postes and other corn products--corn and amaranth biscuit, corn beverage: 0.9, 1.1 and 0.3 micrograms/person/day (mean) respectively.
authors.affiliation: Centre for the Hygiene of Food Chains, National Institute of Public Health, Prague, Czech Republic.
authors.forename: V
authors.initials: V
authors.lastname: Ostrý
authors.forename: J
authors.initials: J
authors.lastname: Ruprich
mesh_terms: D002446:Celiac Disease / Q000178:diet therapy*; D018153:Czech Republic; D004797:Enzyme-Linked Immunosorbent Assay; D005670:Fusarium / Q000302:isolation & purification*; D006801:Humans; D009183:Mycotoxins / Q000302:isolation & purification*; D003313:Zea mays / Q000382:microbiology*
chemical_list: D009183:Mycotoxins