Early hepatocyte, endothelial, and bile duct cell injury after pediatric liver transplantation from cadaveric or living-related donors.
BACKGROUND
When compared with cadaveric grafts (Cad), the potential advantages of pediatric orthotopic liver transplantation (OLT) from living-related (LR) donors may include better graft quality, shorter ischemic time, appropriate preparation of the recipient, and better immunologic compatibility.


METHODS
The aim of this study was to analyze early hepatocyte, endothelial, and bile duct cell injury following pediatric OLT using LR (n=15) or uncomplicated Cad reduced-size (n=10) grafts. Median (range) total ischemic times were 190 min (105-261) versus 760 min (418-948) in LR and Cad groups, respectively (P<0.001).


RESULTS
The post-OLT cytolytic profile, assessed daily during the first 7 days using both plasma glutamate-pyruvate transaminase and serum alpha-glutathione S-transferase, showed significantly higher levels of both parameters for the 10 uncomplicated Cad cases when compared with the 15 LR grafts (P<0.001). The evaluation of hepatic endothelial cell function during the first week after OLT, using serum hyaluronic acid levels, suggested lower endothelial injury in the LR grafts, when compared with the Cad grafts (P=0.059). Bile duct cell injury, as assessed using plasma gamma-glutamyl transferase levels, was similar in both groups, with a progressive increase at the end of the first week after OLT, which was correlated with a similar incidence of early acute rejection in both groups (80% in the LR group vs. 62% in the Cad group, NS).


CONCLUSIONS
(1) The hepatocellular and endothelial cell damage was reduced after OLT with LR grafts, which may be related to shorter ischemic time when compared with Cad grafts; (2) the putative immunologic advantage for LR grafts was not confirmed in terms of incidence of acute rejection.
Reding
R
R
Department of Pediatric Surgery, St-Luc University Clinics, University of Louvain Medical School, Brussels, Belgium.
Wallemacq
P
P
Moulin
D
D
Manicourt
D
D
Lambotte
L
L
Jamart
J
J
Sokal
E
E
de Ville de Goyet
J
J
Otte
J B
JB
D000328:Adult; D000367:Age Factors; D001652:Bile Ducts / Q000473:pathology / Q000502:physiology; D001663:Bilirubin / Q000378:metabolism; D002102:Cadaver; D002648:Child; D002675:Child, Preschool; D004727:Endothelium / Q000473:pathology / Q000502:physiology; D006801:Humans; D006820:Hyaluronic Acid / Q000378:metabolism; D007223:Infant; D008099:Liver / Q000473:pathology*; D008111:Liver Function Tests; D016031:Liver Transplantation / Q000379:methods*; D019520:Living Donors; D013997:Time Factors; D014019:Tissue Donors
D006820:Hyaluronic Acid; D001663:Bilirubin