Extracellular dextran-induced p-nitrophenyl-alpha-D-glucoside-hydrolyzing enzyme of Bacillus circulans KA-304: a producer of Schizophyllum commune-lytic enzyme.
p-NP-alpha-D-Glucoside-hydrolyzing activity in the culture filtrate of Bacillus circulans KA-304, a producer of Schizophyllum commune cell-wall lytic enzyme, increased remarkably when the bacterium was grown on dextran as a carbon source. It was suggested that the increase of the activity was caused by increases of two major species, alpha-D-glucosidase I and alpha-D-glucosidase II. alpha-D-Glucosidase I, which showed a certain reactivity toward dextran, was isolated from the filtrate (MW 70 kDa, 35-fold, 10% recovery). The enzyme was stable around pH 6.5-7.5 and showed its highest activity at pH 6.5. The enzyme preparation inactivated with p-chloromerucuribenzoic acid recovered its activity by incubating with ditiothereitol. Its substrate specificity suggested that the enzyme was an exo-type enzyme with certain affinity toward alpha-1,6-glucosidic linkage.
Mizuno
K
K
Department of Bioscience and Biotechnology, Faculty of Science and Engineering, Ritsumeikan University, Kusatsu, Japan.
Tachiki
T
T
D001407:Bacillus / Q000201:enzymology*; D002729:Chloromercuribenzoates / Q000737:chemistry; D002847:Chromatography, Agarose; D002855:Chromatography, Thin Layer; D003911:Dextrans / Q000378:metabolism*; D004229:Dithiothreitol / Q000737:chemistry; D004791:Enzyme Inhibitors / Q000737:chemistry; D005960:Glucosides / Q000378:metabolism*; D006868:Hydrolysis; D008970:Molecular Weight; D009596:Nitrophenols / Q000032:analysis; D012567:Schizophyllum / Q000201:enzymology*; D013379:Substrate Specificity; D013439:Sulfhydryl Reagents / Q000737:chemistry; D000520:alpha-Glucosidases / Q000302:isolation & purification / Q000378:metabolism*; D020245:p-Chloromercuribenzoic Acid
D002729:Chloromercuribenzoates; D003911:Dextrans; D004791:Enzyme Inhibitors; D005960:Glucosides; D009596:Nitrophenols; D013439:Sulfhydryl Reagents; C019502:4-nitrophenyl alpha-glucoside; D020245:p-Chloromercuribenzoic Acid; C034251:4-nitrophenyl-alpha-glucosidase; C042059:glucosidase I; D000520:alpha-Glucosidases; D004229:Dithiothreitol; C024836:4-nitrophenol