title: [The isolation and subtyping of the human immunodeficiency virus (HIV) from children in Moldova. The evidence for subtype F].
abstract: HIV epidemic related to nosocomial transmission in Romanian nursed children represents up to now more than 50% of paediatric AIDS cases in Europe. Although the sources of this epidemic are obscure, HIV-1 subtype F, a minor form in Brasil and Africa, realised a founder effect in this risk group, thus becoming the major form in Romanian epidemic. This study presents the results of the virus isolation from HIV-infected children in Northeast Romania. Heteroduplex mobility assay (HMA) was performed in order to establish HIV-1 viral subtype for 6 isolates. All tested HIV-1 strains were proved to belong to F subtype. So, our study confirm that HIV-1 subtype F is responsible for the epidemic in the risk group of Romanian nosocomially infected children.
authors.affiliation: Laboratorul de Virologie, Universitatea de Medicină şi Farmacie Gr. T. Popa, Iaşi.
authors.forename: C
authors.initials: C
authors.lastname: Apetrei
authors.forename: G
authors.initials: G
authors.lastname: Collin
authors.forename: D
authors.initials: D
authors.lastname: Diculencu
authors.forename: I L
authors.initials: IL
authors.lastname: Ajaka
authors.forename: I
authors.initials: I
authors.lastname: Farfara
authors.forename: C
authors.initials: C
authors.lastname: Pânzaru
authors.forename: G
authors.initials: G
authors.lastname: Coman
authors.forename: M
authors.initials: M
authors.lastname: Duca
authors.forename: F
authors.initials: F
authors.lastname: Brun-Vezinet
authors.forename: F
authors.initials: F
authors.lastname: Simon
mesh_terms: D000163:Acquired Immunodeficiency Syndrome / Q000821:virology; D001483:Base Sequence; D002648:Child; D017931:DNA Primers; D004279:DNA, Viral / Q000235:genetics; D005838:Genotype; D015658:HIV Infections / Q000821:virology; D006679:HIV Seropositivity / Q000821:virology; D015497:HIV-1 / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D006801:Humans; D008966:Moldova; D008969:Molecular Sequence Data; D009692:Nucleic Acid Heteroduplexes / Q000235:genetics; D014776:Virus Cultivation / Q000379:methods
chemical_list: D017931:DNA Primers; D004279:DNA, Viral; D009692:Nucleic Acid Heteroduplexes
vernacular_title: Izolarea şi subtiparea virusului imunodeficienţei umane (HIV) la copii din Moldova. Evidenţierea subtipului F.