title: Lesions of the nucleus basalis magnocellularis do not alter the proportions of pirenzepine- and gallamine-sensitive responses of somatosensory cortical neurones to acetylcholine in the rat.
abstract: The effects of S-alpha-amino-3-hydroxy-4-isoxozolepropionic acid (AMPA) lesions of the nucleus basalis magnocellularis on the M1/M2 nature of the responses of somatosensory cortical neurones to acetylcholine (ACh) in Sprague-Dawley rats were investigated by iontophoretic application and extracellular single unit recording. The responses were characterised using pirenzepine, an M1 receptor antagonist, and gallamine, an M2 antagonist. Eighty two neurones in control and 94 neurones in lesioned animals were studied. In control animals, 37% of responses to ACh were sensitive to pirenzepine, gallamine or to both antagonists. This increased to 62% in lesioned animals, the proportions of pirenzepine- and gallamine-sensitive responses remaining unchanged. These results provide the first electrophysiological confirmation that both pirenzepine- and gallamine-sensitive (M1 and M2) receptors occur postsynaptic to afferent cholinergic terminals and that their postsynaptic stimulation may produce both inhibition and excitation.
authors.affiliation: Department of Ontogenesis, Russian Academy of Sciences, Moscow, Russian Federation.
authors.forename: V V
authors.initials: VV
authors.lastname: Raevsky
authors.forename: G S
authors.initials: GS
authors.lastname: Dawe
authors.forename: J D
authors.initials: JD
authors.lastname: Sinden
authors.forename: J D
authors.initials: JD
authors.lastname: Stephenson
mesh_terms: D000109:Acetylcholine / Q000494:pharmacology*; D000818:Animals; D005703:Gallamine Triethiodide / Q000494:pharmacology*; D008297:Male; D018727:Muscarinic Antagonists / Q000494:pharmacology*; D009474:Neurons / Q000187:drug effects*; D010890:Pirenzepine / Q000494:pharmacology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D013003:Somatosensory Cortex / Q000187:drug effects* / Q000473:pathology / Q000503:physiopathology; D013377:Substantia Innominata / Q000502:physiology*
chemical_list: D018727:Muscarinic Antagonists; D010890:Pirenzepine; D000109:Acetylcholine; D005703:Gallamine Triethiodide