title: Can grafting of an octapeptide improve the structure of a de novo protein?
abstract: Structural properties and conformational stability of de novo proteins -- albebetin and albeferon (albebetin with a grafted interferon fragment) -- were studied by means of CD spectroscopy, gel filtration and urea-induced unfolding. The results allow us to conclude that albebetin possesses the properties of the molten globule state. Grafting of the octapeptide to the N-terminus of this de novo protein affects its structure. We show here that albeferon maintains a secondary structure content of albebetin; it becomes more compact and much more stable toward urea-induced unfolding as compared to albebetin and even possesses some weak tertiary structure (at least around Tyr7). This means that the structure of the artificial protein albebetin can be improved by a simple procedure of octapeptide grafting to its N-terminus.
authors.affiliation: Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Moscow. mini@siobc.ras.ru
authors.forename: I Y
authors.initials: IY
authors.lastname: Aphasizheva
authors.forename: D A
authors.initials: DA
authors.lastname: Dolgikh
authors.forename: Z K
authors.initials: ZK
authors.lastname: Abdullaev
authors.forename: V N
authors.initials: VN
authors.lastname: Uversky
authors.forename: M P
authors.initials: MP
authors.lastname: Kirpichnikov
authors.forename: O B
authors.initials: OB
authors.lastname: Ptitsyn
mesh_terms: D002850:Chromatography, Gel; D002942:Circular Dichroism; D003001:Cloning, Molecular; D004926:Escherichia coli / Q000235:genetics; D009842:Oligopeptides / Q000737:chemistry*; D015202:Protein Engineering; D017510:Protein Folding; D017433:Protein Structure, Secondary; D017434:Protein Structure, Tertiary; D011506:Proteins / Q000737:chemistry* / Q000235:genetics; D011994:Recombinant Proteins / Q000737:chemistry / Q000235:genetics; D013816:Thermodynamics; D014508:Urea / Q000737:chemistry
chemical_list: D009842:Oligopeptides; D011506:Proteins; D011994:Recombinant Proteins; C074146:albebetin; D014508:Urea