Activated adult human alveolar bone cells: a new model of matrix mineralization.
Activated adult human alveolar bone cells were isolated from 2-wk-old osteogenic tissue recuperated from dental implant surgeries following a two-step procedure. Osteogenic tissues were cultured as explant for 2 months. Cells began to migrate in the first 3 d and were confluent in 3-4 wk. However, adjacent to the explants, multicellular cell layers began to form in 10 d, and matrix mineralization was observed by 4 wk in these areas. These formations enlarged and by the end of the culture period, large diffuse matrix mineralization areas were observed. Light and electron microscopic observations confirmed the presence of a collagen matrix undergoing a mineralization process but showing important differences with the mineralized matrix tissue formed with a rat embryo calvaria bone cell system. This new model, using activated human alveolar bone cells, may provide a tool to investigate alveolar bone development and physiology and to set up new therapeutic approaches.
Nefussi
J R
JR
Laboratoire de Biologie-Odontologie, Département de Chirurgie, Université Paris VII, France.
Casamajor
P
P
Serfaty
R
R
Bolle
M
M
Hugly
C
C
Forest
N
N
D000328:Adult; D000539:Alveolar Process / Q000166:cytology* / Q000378:metabolism* / Q000601:surgery; D000818:Animals; D001857:Bone Matrix / Q000166:cytology* / Q000378:metabolism* / Q000601:surgery; D002478:Cells, Cultured; D015921:Dental Implants; D005260:Female; D006801:Humans; D008297:Male; D008854:Microscopy, Electron; D008875:Middle Aged; D008903:Minerals / Q000378:metabolism*; D008954:Models, Biological; D010012:Osteogenesis / Q000502:physiology; D051381:Rats; D014945:Wound Healing / Q000502:physiology
D015921:Dental Implants; D008903:Minerals