Neutralizing antibodies in sera from macaques infected with chimeric simian-human immunodeficiency virus containing the envelope glycoproteins of either a laboratory-adapted variant or a primary isolate of human immunodeficiency virus type 1.
The magnitude and breadth of neutralizing antibodies raised in response to infection with chimeric simian-human immunodeficiency virus (SHIV) in rhesus macaques were evaluated. Infection with either SHIV-HXB2, SHIV-89.6, or SHIV-89.6PD raised high-titer neutralizing antibodies to the homologous SHIV (SHIV-89.6P in the case of SHIV-89.6PD-infected animals) and significant titers of neutralizing antibodies to human immunodeficiency virus type 1 (HIV-1) strains MN and SF-2. With few exceptions, however, titers of neutralizing antibodies to heterologous SHIV were low or undetectable. The antibodies occasionally neutralized heterologous primary isolates of HIV-1; these antibodies required >40 weeks of infection to reach detectable levels. Notable was the potent neutralization of the HIV-1 89.6 primary isolate by serum samples from SHIV-89.6-infected macaques. These results demonstrate that SHIV-HXB2, SHIV-89.6, and SHIV-89.6P possess highly divergent, strain-specific neutralization epitopes. The results also provide insights into the requirements for raising neutralizing antibodies to primary isolates of HIV-1.
Montefiori
D C
DC
Department of Surgery, Duke University Medical Center, Durham, North Carolina 27710, USA. monte005@mc.duke.edu
Reimann
K A
KA
Wyand
M S
MS
Manson
K
K
Lewis
M G
MG
Collman
R G
RG
Sodroski
J G
JG
Bolognesi
D P
DP
Letvin
N L
NL
D000222:Adaptation, Physiological; D000818:Animals; D002461:Cell Line, Transformed; D015686:Gene Products, env / Q000276:immunology*; D014644:Genetic Variation; D006023:Glycoproteins / Q000276:immunology*; D015483:HIV Antibodies / Q000097:blood / Q000276:immunology*; D015497:HIV-1 / Q000276:immunology* / Q000302:isolation & purification; D006801:Humans; D008253:Macaca mulatta; D009500:Neutralization Tests; D015302:Simian Immunodeficiency Virus / Q000276:immunology*
D015686:Gene Products, env; D006023:Glycoproteins; D015483:HIV Antibodies