Experimental odontogenic cysts induced by in vitro 4-nitroquinoline 1-oxide (4NQO) treatment of F344 rat incisor tooth germs.
This study was designed to establish an experimental animal model for elucidating the early stages of odontogenic cysts and tumors. It involves the in vitro treatment of tooth germs with 4-nitroquinoline 1-oxide (4NQO) at the early bell stage and their subsequent transplantation into the kidney subcapsular space. While all tooth germ transplants of the control group not exposed to the carcinogen showed continued tooth development with no pathological lesions, 21 of 23 4NQO-treated tooth germs developed into similar appearing keratinized cysts with or without associated tooth structures. The remaining two transplants failed to develop cysts and formed only a tooth. The present experimental procedure was effective in inducing keratinized cystic lesions that exhibit some similarities to human odontogenic keratocysts or primordial cysts.
Miura
Y
Y
Department of Oral Pathology, Kagoshima University Dental School, Japan.
Ozaki
H S
HS
Li
T J
TJ
Uemura
M
M
Kitano
M
M
D015112:4-Nitroquinoline-1-oxide / Q000009:adverse effects*; D000565:Ameloblasts / Q000187:drug effects / Q000473:pathology; D000818:Animals; D002273:Carcinogens / Q000009:adverse effects*; D002454:Cell Differentiation; D003743:Dental Enamel / Q000187:drug effects / Q000473:pathology; D003804:Dentin / Q000187:drug effects / Q000473:pathology; D004195:Disease Models, Animal; D004848:Epithelium / Q000187:drug effects / Q000473:pathology; D006085:Graft Survival; D006801:Humans; D007180:Incisor / Q000187:drug effects / Q000473:pathology / Q000637:transplantation; D007668:Kidney / Q000601:surgery; D009804:Odontoblasts / Q000187:drug effects / Q000473:pathology; D009805:Odontogenesis / Q000187:drug effects; D009807:Odontogenic Cysts / Q000139:chemically induced* / Q000473:pathology; D009808:Odontogenic Tumors / Q000139:chemically induced / Q000473:pathology; D009924:Organ Culture Techniques; D051381:Rats; D011916:Rats, Inbred F344; D014083:Tooth Germ / Q000187:drug effects* / Q000473:pathology / Q000637:transplantation
D002273:Carcinogens; D015112:4-Nitroquinoline-1-oxide