Isolation and characterization of site-specific endonuclease Bsp123 I from thermophilic strain Bacillus species 123.
The preparation of site-specific endonuclease Bsp123 I was isolated and purified from the thermophilic strain Bacillus species 123. Endonuclease Bsp123 I recognizes the sequence CGCG and cleaves it in the middle between nucleotides G and C, producing blunt ends. Thus, Bsp123 I is an isoschizomer of FnuDII. The maximal activity of the enzyme is displayed at 42 degrees C, pH 8.0, 100 mM NaCl, 10 mM MgCl2.
Alzhanova
D V
DV
Institute of Protein Research, Russian Academy of Sciences, Pushchino, Moscow Region, 142292 Russia. nikmatv@sun.ipr.serpukhov.su
Alzhanov
D T
DT
Zheleznaya
L A
LA
Matvienko
N I
NI
D001407:Bacillus / Q000201:enzymology*; D004279:DNA, Viral / Q000378:metabolism; D015252:Deoxyribonucleases, Type II Site-Specific / Q000302:isolation & purification* / Q000378:metabolism; D013379:Substrate Specificity
D004279:DNA, Viral; C039566:CGCG-specific type II deoxyribonucleases; D015252:Deoxyribonucleases, Type II Site-Specific