Organic solvent extraction associated with HPLC in the preparation of hemorphins from bovine hemoglobin peptic hydrolysate.
Hemorphins extraction by organic solvents from a hemoglobin peptic hydrolysate was investigated. About thirteen solvents were used and only the aliphatic alcohols displayed selectivity for hemorphins. The resulting extracted phases, analyzed by SE-HPLC, RP-HPLC, and mass spectrometry, proved 1-butanol and 2-butanol to be the best extracting solvents towards hemorphins. The opioid activity test was carried out following each step of extraction and the obtained results were in agreement with a purification.
Zhao
Q
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Laboratoire de Génie Protéique et Cellulaire, Pôle Sciences et Technologies, Université de La Rochelle, France.
Piot
J M
JM
D000438:Alcohols / Q000378:metabolism; D000818:Animals; D002417:Cattle; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D006168:Guinea Pigs; D006454:Hemoglobins / Q000737:chemistry* / Q000378:metabolism; D007082:Ileum / Q000187:drug effects; D009294:Narcotics / Q000032:analysis / Q000302:isolation & purification*; D010434:Pepsin A / Q000378:metabolism; D010446:Peptide Fragments / Q000302:isolation & purification* / Q000494:pharmacology; D012997:Solvents / Q000737:chemistry; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D013053:Spectrophotometry
D000438:Alcohols; D006454:Hemoglobins; D009294:Narcotics; D010446:Peptide Fragments; D012997:Solvents; D010434:Pepsin A