GATA factor-dependent regulation of cardiac m2 muscarinic acetylcholine gene transcription.
The m2 subtype is the predominant muscarinic acetylcholine receptor subtype expressed in heart and regulates the rate and force of cardiac contraction. We have previously reported the isolation of the promoter region for the chick m2 receptor gene and defined a region of the chick m2 promoter sufficient for high level expression in cardiac primary cultures. In this manuscript we demonstrate transactivation of cm2 promoter by the GATA family of transcription factors. In addition, we define the GATA-responsive element in the chick m2 promoter and demonstrate that this element is required for expression in cardiac primary cultures. Finally, we demonstrate specific binding of both a chick heart nuclear protein and of cloned chick GATA-4, -5, and -6 to the GATA-responsive element.
Rosoff
M L
ML
Department of Pharmacology, University of Washington, Seattle, Washington 98195-7750, USA.
Nathanson
N M
NM
D000595:Amino Acid Sequence; D000818:Animals; D019556:COS Cells; D002460:Cell Line; D002478:Cells, Cultured; D002642:Chick Embryo; D017124:Conserved Sequence; D004268:DNA-Binding Proteins / Q000737:chemistry / Q000378:metabolism*; D005786:Gene Expression Regulation; D009206:Myocardium / Q000378:metabolism*; D011401:Promoter Regions, Genetic; D043585:Receptor, Muscarinic M2; D011976:Receptors, Muscarinic / Q000096:biosynthesis* / Q000235:genetics; D011993:Recombinant Fusion Proteins / Q000378:metabolism; D014157:Transcription Factors / Q000737:chemistry / Q000378:metabolism*; D014158:Transcription, Genetic; D015533:Transcriptional Activation; D014162:Transfection; D016335:Zinc Fingers
D004268:DNA-Binding Proteins; D043585:Receptor, Muscarinic M2; D011976:Receptors, Muscarinic; D011993:Recombinant Fusion Proteins; D014157:Transcription Factors