Rapid diagnosis of simian varicella using the polymerase chain reaction.
Simian varicella virus (SVV) causes sporadic epizootics of a varicella-like disease in nonhuman primates. Rapid diagnosis of simian varicella is critical in controlling epizootics. A polymerase chain reaction (PCR)-based diagnostic assay for detection of SVV DNA in cell culture and clinical samples from SVV-infected monkeys was developed. The assay is rapid, specific, and highly sensitive. The SVV DNA is readily detected in skin rash specimens and in peripheral blood lymphocytes of infected monkeys during the early stages of clinical varicella. In addition to providing an important diagnostic tool, the SVV PCR assay is also useful for investigating the epidemiology and pathogenesis of simian varicella.
Gray
W L
WL
Department of Microbiology and Immunology, University of Arkansas for Medical Sciences, Little Rock 72205, USA.
Williams
R J
RJ
Soike
K F
KF
D000818:Animals; D002644:Chickenpox / Q000175:diagnosis / Q000662:veterinary*; D002522:Chlorocebus aethiops; D004279:DNA, Viral / Q000032:analysis* / Q000097:blood; D006564:Herpesviridae / Q000235:genetics* / Q000302:isolation & purification; D007962:Leukocytes / Q000821:virology; D008992:Monkey Diseases / Q000821:virology*; D016133:Polymerase Chain Reaction; D012867:Skin / Q000821:virology; D014709:Vero Cells
D004279:DNA, Viral