title: Experimental traumatic cerebral contusion: morphological study of brain microvessels and characterization of the oedema.
abstract: Several experimental brain oedema models are currently available, but most of them are very different from what happens in clinical practice. As it is simple and seems to replicate the range of injuries seen in man we decided to evaluate Marmarou's model of head injury in order to test physiopathogenic and therapeutic hypotheses. Three groups of Wistar rats weighting 360-400 gr, anaesthetized with sodium pentobarbitone and breathing spontaneously, without tracheal intubation, were studied. In the first group six animals were killed two hours after injury and the brain's water content compared with that of nine controls. In another group Evans blue (100 mg/kg) was injected one hour before trauma and dye's extraction ratio determined at various times after injury: five animals at 15 minutes, six at 30 minutes, five at 60 minutes and nine at 120 minutes. A total of twenty-eight animals served as controls. In the last group morphological studies with light and electron microscopy were performed in the traumatized brain tissue from rats killed 5 and 120 minutes after injury and in brain tissue from control rats. Results showed that Marmarou's brain trauma model induced perivascular brain oedema, already visible at the ultrastructural level 5 minutes after the injury. Endothelial cells themselves were "oedematiated", rich in pinocytotic vesicles and membrane blebs, and presented intact tight junctions. Two hours after trauma the perivascular oedema was more marked. At this time the brain water content was significantly higher than that in controls. Evans blue extraction ratio increased linearly with time, being significantly higher than in controls 120 minutes after injury. We conclude that Marmarou's model is a suitable model for the study of brain oedema induced by trauma, and that this oedema, assessed by three different methodologies, was statistically significant two hours after injury.
authors.affiliation: Institute of Pharmacology and Therapeutics, Mesical Faculty, Hospital S. Joäo, Porto, Portugal.
authors.forename: R
authors.initials: R
authors.lastname: Vaz
authors.forename: A
authors.initials: A
authors.lastname: Sarmento
authors.forename: N
authors.initials: N
authors.lastname: Borges
authors.forename: C
authors.initials: C
authors.lastname: Cruz
authors.forename: T
authors.initials: T
authors.lastname: Azevedo
mesh_terms: D000818:Animals; D001808:Blood Vessels / Q000473:pathology; D001929:Brain Edema / Q000209:etiology* / Q000473:pathology*; D002560:Cerebrovascular Circulation / Q000502:physiology; D004396:Coloring Agents; D003288:Contusions / Q000209:etiology* / Q000473:pathology*; D006259:Craniocerebral Trauma / Q000150:complications*; D004195:Disease Models, Animal; D005070:Evans Blue; D008833:Microcirculation / Q000502:physiology; D051381:Rats; D017208:Rats, Wistar
chemical_list: D004396:Coloring Agents; D005070:Evans Blue