title: Absence of RAS and p53 mutations in thyroid carcinomas of children after Chernobyl in contrast to adult thyroid tumours.
abstract: Thyroid carcinomas of an additional series of 34 children exposed to radioactive fall-out after the Chernobyl reactor accident were analysed for mutations in the H-, K- and N-RAS and the p53 gene. Allele-specific oligonucleotide hybridization, single-strand conformation polymorphism (SSCP) and direct sequencing did not disclose mutations in codons 12, 13 and 61 of RAS genes nor mutations in exons 5, 7 and 8 of p53. Considering the recently reported high prevalence of RET rearrangements of the PTC3 type in childhood tumours after Chernobyl (Klugbauer et al, 1995, Oncogene 11: 2459-2467), it follows that RET rearrangements are the most relevant molecular aberration in these radiation-induced tumours. RAS or p53 mutations do not play a role in childhood thyroid carcinogenesis after Chernobyl.
authors.affiliation: Institute of Pathology, University of Munich, Germany.
authors.forename: B
authors.initials: B
authors.lastname: Suchy
authors.forename: V
authors.initials: V
authors.lastname: Waldmann
authors.forename: S
authors.initials: S
authors.lastname: Klugbauer
authors.forename: H M
authors.initials: HM
authors.lastname: Rabes
mesh_terms: D000328:Adult; D000367:Age Factors; D001483:Base Sequence; D002648:Child; D005091:Exons; D015321:Gene Rearrangement; D005809:Genes, Regulator; D016158:Genes, p53; D011905:Genes, ras; D006801:Humans; D016296:Mutagenesis; D009381:Neoplasms, Radiation-Induced / Q000209:etiology / Q000235:genetics*; D016133:Polymerase Chain Reaction; D018807:Polymorphism, Single-Stranded Conformational; D011847:Radioactive Fallout; D018788:Radioactive Hazard Release; D002094:Republic of Belarus; D013964:Thyroid Neoplasms / Q000209:etiology / Q000235:genetics*; D014455:Ukraine
chemical_list: D011847:Radioactive Fallout