Recommended storage and resuscitation conditions for the MDCC-MSB1 cell line.
Storage and resuscitation of MDCC-MSB1 cells, most commonly used for the propagation of chicken anemia virus was studied. Cells were frozen slowly in RPMI medium 1640 with 10% or 20% fetal bovine serum and 20% dimethyl sulfoxide, held at -70 C overnight, and placed into liquid nitrogen the next day. After 1 mo, 91% to 100% resuscitation rates were obtained, while cells that were frozen fast (directly transferred into liquid nitrogen) could not be resuscitated.
Coombes
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Biomedical Research Group, School of Biomedical Sciences, Charles Sturt University, Wagga Wagga, New South Wales, Australia.
Crawford
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GR
D000818:Animals; D001769:Blood; D002417:Cattle; D018929:Cell Culture Techniques / Q000379:methods*; D002460:Cell Line; D002470:Cell Survival; D017727:Chicken anemia virus / Q000254:growth & development*; D002645:Chickens; D015925:Cryopreservation / Q000379:methods*; D003470:Culture Media
D003470:Culture Media