Expression of a 260 kDa neuroblastoma surface antigen, the target of cytotoxic natural human IgM: correlation to MYCN amplification and effects of retinoic acid.
Human neuroblastoma cells contain a 260 kDa surface-associated antigen (NB-p260) that is recognised by natural cytotoxic IgM antibodies. In this study we demonstrate that NB-p260 is expressed in vivo in a neuroblastoma tumour specimen but not in normal human tissues of neuronal origin. Since MYCN amplification is a clinical marker of neuroblastoma disease progression, we analysed the expression of NB-p260 in human neuroblastoma cell lines with different MYCN amplification status. However, both amplified and non-amplified neuroblastoma cell lines exhibited comparable NB-p260 expression. Treatment of neuroblastoma cells with the differentiation-inducing agent retinoic acid (RA) also had no effect on the expression of NB-p260. Collectively, the data suggest that expression of NB-p260 on human neuroblastoma cells is independent of malignancy and differentiation status of neuroblastoma.
David
K
K
Department of Biochemistry and Molecular Biology, University of Hamburg, Germany.
Ehrhardt
A
A
Ollert
M W
MW
Erttmann
R
R
Bredehorst
R
R
Vogel
C W
CW
D000951:Antigens, Neoplasm / Q000276:immunology / Q000378:metabolism*; D000954:Antigens, Surface / Q000276:immunology / Q000378:metabolism*; D002471:Cell Transformation, Neoplastic; D005784:Gene Amplification; D015870:Gene Expression; D016259:Genes, myc / Q000235:genetics*; D006801:Humans; D015151:Immunoblotting; D007075:Immunoglobulin M / Q000276:immunology*; D007150:Immunohistochemistry; D009447:Neuroblastoma / Q000235:genetics / Q000276:immunology / Q000378:metabolism* / Q000473:pathology; D014212:Tretinoin / Q000494:pharmacology*; D014407:Tumor Cells, Cultured / Q000187:drug effects
D000951:Antigens, Neoplasm; D000954:Antigens, Surface; D007075:Immunoglobulin M; D014212:Tretinoin