Specific recombinogenic activity of a new polyene antibiotic.
A new antibiotic from Streptomyces sp., tetrapol A159, active against various fungi, a promising compound for the control of plant diseases, was studied for its genotoxic effects. It was produced at the Institute of Microbiological Preparations for Agriculture, Sofia, Bulgaria. The chemical was tested in three different test systems: a bacterial system, the Ames test for point mutations, the micronucleus test in bone marrow cells of rats for chromosomal aberrations and the fungal system of Aspergillus nidulans for mitotic recombination and aneuploidy. No increase in histidine revertants was observed in any of the TA100, TA98, TA1535 and TA1537 strains of Salmonella at concentrations ranging from 1 to 4000 mg/plate. The results were also negative in the micronucleus test of bone marrow cells at concentrations from 124 to 600 mg/kg b.w., whereas a statistically significant threefold increase of mitotic crossovers was found in Aspergillus, at concentrations from 0.5 to 2.5 mg/ml.
Vachkova
R
R
National Center of Hygiene and Medical Ecology, Medical Academy, Sofia, Bulgaria.
Kappas
A
A
Tyagunenko
E
E
Markaki
M
M
Voutsinas
G
G
D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology* / Q000633:toxicity; D000935:Antifungal Agents / Q000494:pharmacology / Q000633:toxicity; D001233:Aspergillus nidulans / Q000187:drug effects; D008099:Liver / Q000201:enzymology; D015162:Micronucleus Tests; D009152:Mutagenicity Tests; D011090:Polyenes / Q000494:pharmacology* / Q000633:toxicity; D051381:Rats; D017208:Rats, Wistar; D011995:Recombination, Genetic / Q000235:genetics; D012475:Salmonella / Q000235:genetics; D013302:Streptomyces / Q000737:chemistry*
D000900:Anti-Bacterial Agents; D000935:Antifungal Agents; D011090:Polyenes