Chandelier cell axons are immunoreactive for GAT-1 in the human neocortex.
We have examined the pattern of immunostaining for the high-affinity GABA transporter GAT-1 in the human temporal neocortex. Immunocytochemistry for GAT-1 labels terminal-like puncta in the neuropil and around unstained cell bodies. The characteristic terminal portions of chandelier cell axons (Ch-terminals, which form multiple inhibitory GABAergic synaptic contacts with the axon initial segments of pyramidal cells) were among the strongest immunocytochemically stained elements for GAT-1. Since Ch-terminals are immunoreactive for the calcium-binding protein parvalbumin, experiments were carried out to study the co-localization of GAT-1 and parvalbumin in Ch-terminals. These experiments showed that the vast majority of Ch-terminals immunoreactive for GAT-1 were also immunoreactive for PV. We concluded that GAT-1 transporter may have an important functional role in controlling pyramidal cell activity.
DeFelipe
J
J
Instituto Cajal, CSIC, Madrid, Spain.
González-Albo
M C
MC
D000328:Adult; D001369:Axons / Q000737:chemistry*; D002352:Carrier Proteins / Q000032:analysis*; D050485:GABA Plasma Membrane Transport Proteins; D006801:Humans; D007150:Immunohistochemistry; D007395:Interneurons / Q000737:chemistry* / Q000648:ultrastructure; D008565:Membrane Proteins / Q000032:analysis*; D026901:Membrane Transport Proteins; D019579:Neocortex / Q000737:chemistry* / Q000648:ultrastructure; D009419:Nerve Tissue Proteins / Q000032:analysis*; D027361:Organic Anion Transporters; D017966:Pyramidal Cells / Q000737:chemistry* / Q000648:ultrastructure; D005680:gamma-Aminobutyric Acid / Q000378:metabolism*
D002352:Carrier Proteins; D050485:GABA Plasma Membrane Transport Proteins; D008565:Membrane Proteins; D026901:Membrane Transport Proteins; D009419:Nerve Tissue Proteins; D027361:Organic Anion Transporters; C493413:SLC6A1 protein, human; D005680:gamma-Aminobutyric Acid