Hypothermia attenuates the activation of protein kinase C in focal ischemic rat brain: dual autoradiographic study of [3H]phorbol 12,13-dibutyrate and iodo[14C]antipyrine.
Using phorbol 12,13-dibutyrate (PDBu) autoradiography, we investigated the effect of hypothermia or protein kinase C (PKC) activation in rat brain 2 h after focal ischemia. In normothermia, a significant increase of PDBu binding was observed in ischemic brain. Hypothermia suppressed the increase of PDBu binding in degree and extent. These observations suggest that intraischemic hypothermia attenuates the activation of PKC, and this may in part be participate in the protective effect of hypothermia.
Tohyama
Y
Y
Department of Neurosurgery, Asahikawa Medical College, Japan.
Sako
K
K
Yonemasu
Y
Y
D000818:Animals; D000983:Antipyrine / Q000031:analogs & derivatives* / Q000378:metabolism; D001345:Autoradiography; D001921:Brain / Q000378:metabolism*; D002545:Brain Ischemia / Q000378:metabolism* / Q000503:physiopathology; D002250:Carbon Radioisotopes; D002560:Cerebrovascular Circulation / Q000502:physiology; D004789:Enzyme Activation / Q000502:physiology; D007036:Hypothermia, Induced; D008297:Male; D015240:Phorbol 12,13-Dibutyrate / Q000378:metabolism*; D011493:Protein Kinase C / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar; D014316:Tritium
D002250:Carbon Radioisotopes; D014316:Tritium; D015240:Phorbol 12,13-Dibutyrate; D011493:Protein Kinase C; D000983:Antipyrine; C008135:iodoantipyrine