[Detection of nucleic amino acid enantiomers by HPLC with preliminary modification by o-phthalic aldehyde].
The use of reversed-phase HPLC with UV detection at 254 nm for the separation of stereoisomers of nucleoamino acids, namely, pyrimidyl and purinyl derivatives of alanine, after premodification with o-phthalic aldehyde and N-acetyl-L-cysteine was studied. The use of 0.01 M phosphate buffer (pH 7.0) containing 5% acetonitrile as a mobile phase resulted in satisfactory separation. The range of the detectable amounts of nucleoamino acids was 0.08-1.0 nmol. This method was used for monitoring the formation of stereoisomers in the hydrolysis of N-phenylacetylcytosinylalanine.
Gurentsova
O I
OI
Belozersky Institute of Physicochemical Biology, Moscow State University, Russia.
Savchenko
M V
MV
Sumbatian
N V
NV
Korshunova
G A
GA
Shviadas
V K
VK
D000111:Acetylcysteine / Q000737:chemistry*; D000409:Alanine / Q000031:analogs & derivatives* / Q000737:chemistry*; D000447:Aldehydes; D002021:Buffers; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D006863:Hydrogen-Ion Concentration; D006868:Hydrolysis; D011687:Purines / Q000737:chemistry*; D011743:Pyrimidines / Q000737:chemistry*; D013056:Spectrophotometry, Ultraviolet; D013237:Stereoisomerism; D009764:o-Phthalaldehyde / Q000737:chemistry*
D000447:Aldehydes; D002021:Buffers; D011687:Purines; D011743:Pyrimidines; D009764:o-Phthalaldehyde; D000409:Alanine; D000111:Acetylcysteine
Opredelenie énantiomerov nucleoaminokislot metodom vysoko'effektivnoĭ zhidkostnoĭ khromatografii s predkolonochnoĭ modifikatsieĭ o-ftalevym al'degidom.