Inhibition of human erythrocyte (Na(+)-K+)ATPase by organic hydroperoxides and protection by ascorbic acid and butylated hydroxytoluene.
1. The in vitro effects of cumene hydroperoxide and t-butyl hydroperoxide on intact human erythrocyte membrane (Na(+)-K+)ATPase activities have been studied. 2. (Na(+)-K+)ATPase activities on erythrocyte membranes decreased in agreement with the results of chemiluminescence experiments. 3. Our results demonstrated that the organic hydroperoxides inhibit the activity of (Na(+)-K+) ATPase enzyme and that the antioxidants used prevent this inhibition.
Yeşilkaya
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Department of Biochemistry, School of Medicine, Akdeniz University, Antalya, Turkey.
Yeğin
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D000891:Anti-Infective Agents, Local / Q000494:pharmacology*; D000975:Antioxidants / Q000494:pharmacology*; D001205:Ascorbic Acid / Q000494:pharmacology*; D002084:Butylated Hydroxytoluene / Q000494:pharmacology*; D004912:Erythrocytes / Q000201:enzymology*; D006801:Humans; D006861:Hydrogen Peroxide / Q000494:pharmacology*; D000254:Sodium-Potassium-Exchanging ATPase / Q000037:antagonists & inhibitors*
D000891:Anti-Infective Agents, Local; D000975:Antioxidants; D002084:Butylated Hydroxytoluene; D006861:Hydrogen Peroxide; D000254:Sodium-Potassium-Exchanging ATPase; D001205:Ascorbic Acid