title: Calcium inflow of hamster Merkel cells in response to hyposmotic stimulation indicate a stretch activated ion channel.
abstract: The aim of this study was to investigate the existence of the stretch activated ion channels in single Merkel cell using microfluorimetric techniques. Single Merkel cells were dissociated enzymatically from the touch domes in the cheek pouch mucosa of 4-8 week old golden hamsters. They were loaded with calcium (Ca2+) fluorescent indicator fura-2/AM. The increase in intracellular Ca2+ concentration ([Ca2+]i) of a single Merkel cell (quinacrine fluorescent cell) was induced by hyposmotic solution in normal Krebs solution, while it was not induced by Ca2+-free hyposmotic solution in Ca2+-free physiological solution. Gadolinium ion (10 microM) in normal Krebs solution partially blocked the increase in [Ca2+]i of Merkel cells induced by hyposmotic solution. Hence, this study revealed that stretch activated ion channels existed on the Merkel cell membrane.
authors.affiliation: Department of Physiology, Oral Health Science Center, Tokyo Dental College, Chiba, Japan.
authors.forename: M
authors.initials: M
authors.lastname: Tazaki
authors.forename: T
authors.initials: T
authors.lastname: Suzuki
mesh_terms: D000818:Animals; D002118:Calcium / Q000378:metabolism* / Q000493:pharmacokinetics; D015220:Calcium Channels / Q000502:physiology*; D006224:Cricetinae; D005682:Gadolinium / Q000494:pharmacology; D006982:Hypertonic Solutions; D015640:Ion Channel Gating / Q000502:physiology; D007552:Isotonic Solutions / Q000494:pharmacology; D018862:Merkel Cells / Q000737:chemistry* / Q000502:physiology*; D008647:Mesocricetus; D009092:Mucous Membrane / Q000737:chemistry; D009997:Osmotic Pressure; D012867:Skin / Q000294:innervation*; D014110:Touch / Q000502:physiology
chemical_list: D015220:Calcium Channels; D006982:Hypertonic Solutions; D007552:Isotonic Solutions; C051865:Krebs-Ringer solution; D005682:Gadolinium; D002118:Calcium