title: Polyketomycin, a new antibiotic from Streptomyces sp. MK277-AF1. I. Taxonomy, production, isolation, physico-chemical properties and biological activities.
abstract: A new antibiotic designated polyketomycin was isolated from the culture broth of Streptomyces sp. MK277-AF1. It was purified by ethyl acetate extraction, Sephadex LH-20 column chromatography and centrifugal partition chromatography (CPC). It inhibited growth of Gram-positive bacteria including methicillin-resistant Staphylococcus aureus (MRSA). Its MICs were less than 0.2 microgram/ml. Polyketomycin exhibited cytotoxic activity against nine tumor cell lines at concentrations of 0.9-5.2 micrograms/ml.
authors.affiliation: Institute of Microbial Chemistry, Tokyo, Japan.
authors.forename: I
authors.initials: I
authors.lastname: Momose
authors.forename: W
authors.initials: W
authors.lastname: Chen
authors.forename: N
authors.initials: N
authors.lastname: Kinoshita
authors.forename: H
authors.initials: H
authors.lastname: Iinuma
authors.forename: M
authors.initials: M
authors.lastname: Hamada
authors.forename: T
authors.initials: T
authors.lastname: Takeuchi
mesh_terms: D000900:Anti-Bacterial Agents / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D006038:Glyoxylates / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D016106:Methicillin Resistance; D008826:Microbial Sensitivity Tests; D013211:Staphylococcus aureus / Q000187:drug effects; D013302:Streptomyces / Q000737:chemistry* / Q000145:classification
chemical_list: D000900:Anti-Bacterial Agents; D006038:Glyoxylates; C008231:ketomycin