[A new class of small RNP (alpha-RNP) containing antisense RNA in K-562 cells. IV. The coordinated regulation of the expression of Alu-containing mRNA and alpha-RNA during differentiation].
Small alpha-RNP of K-562 cells contain a small RNA as an RNA component, this RNA is homologous to Alu-repeating sequences of human DNA. When cells are exposed to dimethylsulfoxide, an agent inducing cell differentiation along the erythroid pathway, the content of both high-molecular-weight (heterogeneous nuclear and messenger) RNA enriched with Alu repeats and low-molecular-weight specific RNA, small Alu-homologous alpha-RNA undergoes a coordinated decrease. Using the technique of northern blot hybridization, we have demonstrated nonuniform distribution of Alu repeats both in the fraction of total low-molecular-weight RNA of the cytoplasm as well as in the fraction of messenger RNA. It is proposed that alpha-RNA (alpha-RNP) participates in the control of expression of non-linked Alu-containing genes.
Petukhova
O A
OA
Institute of Cytology, Russian Academy of Sciences, St. Petersburg, Russia.
Mittenberg
A G
AG
Kulichkova
V A
VA
Kozhukharova
I V
IV
Ermolaeva
Iu B
IuB
Gauze
L N
LN
Konstantinova
I M
IM
D015152:Blotting, Northern / Q000379:methods; D002471:Cell Transformation, Neoplastic / Q000187:drug effects / Q000235:genetics; D004121:Dimethyl Sulfoxide / Q000494:pharmacology; D015972:Gene Expression Regulation, Neoplastic / Q000187:drug effects / Q000235:genetics*; D016045:Human Genome Project; D006801:Humans; D004915:Leukemia, Erythroblastic, Acute / Q000235:genetics; D008970:Molecular Weight; D009693:Nucleic Acid Hybridization / Q000379:methods; D010957:Plasmids / Q000235:genetics; D016372:RNA, Antisense / Q000187:drug effects / Q000235:genetics*; D012333:RNA, Messenger / Q000187:drug effects / Q000235:genetics*; D012334:RNA, Neoplasm / Q000187:drug effects / Q000235:genetics*; D012091:Repetitive Sequences, Nucleic Acid / Q000187:drug effects / Q000235:genetics*; D017411:Ribonucleoproteins, Small Nuclear / Q000187:drug effects / Q000235:genetics*; D014158:Transcription, Genetic / Q000187:drug effects / Q000235:genetics; D014407:Tumor Cells, Cultured
D016372:RNA, Antisense; D012333:RNA, Messenger; D012334:RNA, Neoplasm; D017411:Ribonucleoproteins, Small Nuclear; D004121:Dimethyl Sulfoxide
Novyĭ klass malykh RNP (al'fa-RNP), soderzhashchikh antismyslovuiu RNK, v kletkakh linii K-562. IV. Koordinirovannaia reguliatsiia ékspressii Alu-soderzhashchikh mPHK i al'fa-RNK v khode differentsirovki.