Rapid one-step isolation of mouse liver catalase by immobilized metal ion affinity chromatography.
A novel and rapid procedure for the isolation of catalase from mouse liver, after prior treatment with the peroxisome proliferator perfluorooctanoic acid was developed using immobilized metal ion affinity chromatography involving chelation with zinc ions. The purification developed is simple, rapid (requiring 3 hours from cytosol or peroxisomal matrix to homogeneous proteins), reproducible, and yields virtually complete overall recovery of catalase activity. This procedure makes catalase from a variety of tissues and physiological and environmental conditions more readily available for study.
Yang
Q
Q
Department of Biochemisry, Wallenberg Laboratory, Stockholm University, Sweden.
DePierre
J W
JW
D000818:Animals; D002374:Catalase / Q000302:isolation & purification* / Q000378:metabolism; D002413:Cations, Divalent / Q000737:chemistry*; D002614:Chelating Agents / Q000737:chemistry*; D002846:Chromatography, Affinity / Q000379:methods*; D003600:Cytosol / Q000201:enzymology; D004591:Electrophoresis, Polyacrylamide Gel; D007098:Imino Acids / Q000737:chemistry*; D008099:Liver / Q000201:enzymology*; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D008928:Mitochondria / Q000201:enzymology; D013347:Subcellular Fractions / Q000201:enzymology*
D002413:Cations, Divalent; D002614:Chelating Agents; D007098:Imino Acids; D002374:Catalase; C008109:iminodiacetic acid