title: Role of machine perfusion preservation in kidney transplantation from non-heartbeating donors.
abstract: The shortage of kidneys for transplantation is a universal problem. If the viability of the kidney can be assured, organ procurement from non-heartbeating donors will be greatly enhanced. This study evaluates the usefulness of machine perfusion preservation parameters as an index of kidney graft viability. We report our experience with 77 non-heartbeating donor kidneys preserved with machine perfusion technique. Sixty-eight grafts demonstrated excellent perfusion (mean flow 0.79 ml/min/g) with low vascular resistance (55.4 mmHg/ml/min/g). Early graft function occurred in all of these kidneys. Nine kidneys demonstrated poor perfusion (mean flow 0.35 ml/min/g) and elevated pressures with high vascular resistance (132.5 mmHg/ml/min/g). Four kidneys with poor perfusion and elevated pressures was discarded after perfusion. The four mates of these discarded at our center were primarily non-functional when transplanted at another transplant center. All five of the poorly perfused kidneys experienced primary non-function. We conclude that the use of quantitative values of perfusion flow (> 0.4 ml/min/g) and no increased pressure pattern allow safe utilization of grafts from non-heartbeating donors and can predict early postoperative function.
authors.affiliation: The 5th Department of Surgery, Hachioji Medical Center, Tokyo Medical College, Hachioji-city, Japan.
authors.forename: N
authors.initials: N
authors.lastname: Matsuno
authors.forename: E
authors.initials: E
authors.lastname: Sakurai
authors.forename: M
authors.initials: M
authors.lastname: Uchiyama
authors.forename: K
authors.initials: K
authors.lastname: Kozaki
authors.forename: K
authors.initials: K
authors.lastname: Miyamoto
authors.forename: M
authors.initials: M
authors.lastname: Kozaki
authors.forename: T
authors.initials: T
authors.lastname: Nagao
mesh_terms: D006085:Graft Survival; D006323:Heart Arrest; D006801:Humans; D007668:Kidney / Q000502:physiology*; D016030:Kidney Transplantation / Q000502:physiology*; D009926:Organ Preservation / Q000379:methods*; D010477:Perfusion / Q000379:methods*; D013997:Time Factors; D014019:Tissue Donors