The involvement of Ca2+-dependent protein kinase in the regeneration of rice cultured suspension cells.
Short-term cultured cells of rice (Oryza sativa) were found to be capable of regeneration, in contrast to those obtained from long-term cultures. For clarification of the mechanism of regeneration, it was first necessary to distinguish protein kinase activity in long-term and short-term cultured cells; this activity was found greater in the former than latter. The activity was dependent on calcium, not phospholipid, phorbol ester or calmodulin. The apparent Mr of both Ca2+-dependent protein kinases was 32 kDa according to gel phosphorylation. Phosphoserine was identified in serine residues in phosphorylated histone III-S by phosphoamino acid analysis. A Ca2+-dependent protein kinase having a relative Mr of 32 kDa is thus shown to be possibly essential to regeneration in rice cultured cells.
Karibe
H
H
Department of Molecular Biology, National Institute of Agrobiological Resources, Tsukuba, Ibaraki, Japan.
Komatsu
S
S
D002455:Cell Division; D002478:Cells, Cultured; D004591:Electrophoresis, Polyacrylamide Gel; D006657:Histones / Q000378:metabolism; D012275:Oryza / Q000166:cytology* / Q000201:enzymology / Q000378:metabolism; D010766:Phosphorylation; D011494:Protein Kinases / Q000378:metabolism*; D013347:Subcellular Fractions / Q000201:enzymology; D017871:Calcium-Calmodulin-Dependent Protein Kinases
D006657:Histones; D011494:Protein Kinases; D017871:Calcium-Calmodulin-Dependent Protein Kinases