Determination of Ro 48-3656 in rat plasma by reversed-phase high-performance liquid chromatography. Comparison of 1.5-microm nonporous silica to 3.5-microm porous silica analytical columns.
We describe a method for measuring Ro 48-3656 in EDTA rat plasma by neutral pH, reversed-phase high-performance liquid chromatography using a 1.5-microm nonporous silica, C18 analytical column and UV absorbance detection to support pharmacokinetic studies. We also describe a comparison of the 1.5-microm nonporous silica C18 column versus 3.5-microm porous silica C18 columns. The final method using the 1.5-microm nonporous silica column demonstrated good precision (of both quantification and retention time), accuracy and recovery, linearity of dilution and limit of quantification (40 ng/ml Ro 48-3656 using a 20 microl injection). Samples of neat EDTA rat plasma were prepared by ultrafiltration followed by direct injection onto the HPLC column.
Paasch
B D
BD
BioAnalytical Methods Development, Genentech, Inc., South San Francisco, CA 94080, USA.
Lin
Y S
YS
Porter
S
S
Modi
N B
NB
Barder
T J
TJ
D000818:Animals; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004492:Edetic Acid; D005445:Flunitrazepam / Q000031:analogs & derivatives*; D006571:Heterocyclic Compounds; D008297:Male; D008832:Microchemistry; D010091:Oximes; D019039:Platelet Glycoprotein GPIIb-IIIa Complex / Q000037:antagonists & inhibitors*; D011355:Prodrugs; D011786:Quality Control; D051381:Rats; D017207:Rats, Sprague-Dawley; D012680:Sensitivity and Specificity; D012822:Silicon Dioxide
D006571:Heterocyclic Compounds; D010091:Oximes; D019039:Platelet Glycoprotein GPIIb-IIIa Complex; D011355:Prodrugs; C110992:Ro 48-3656; D005445:Flunitrazepam; D012822:Silicon Dioxide; D004492:Edetic Acid