Activation of human T and B cells by rabbit anti-human beta 2-microglobulin.
Rabbit anti-human beta 2-microglobulin (anti-beta 2m) increased the highest DNA synthesis in unseparated lymphocytes or artificially composed mixtures enriched in T and B cells. In enriched T and B cells no or low stimulation was seen. The maximal response by IgG anti-beta 2m was seen in proportions of enriched T/B cells, being 3:1 for blood lymphocytes and 1:1 for spleen cells, which are the same as the physiological proportions of T and B cells in these lymphoid organs. Whereas unseparated lymphocytes gave a peak response on day 3, enriched B cells had a peak response on day 6. Fab anti-beta 2m did not activate enriched T cells but increased DNA synthesis in enriched B cells to about the same extent as unseparated lymphocytes and mixtures of enriched T and B cells. The proportion of sheep erythrocyte rosette-forming cells (E-RFC) decreased after stimulation by anti-beta 2m and increased after stimulation by phytohaemagglutinin. However, as revealed by autoradiography, a proportion of lymphocytes activated by anti-beta 2m were E-RFC and this proportion increased with increasing stimulation by anti-beta 2m. DNA synthesis induced by anti-beta 2m was unchanged for spleen cells and slightly decreased for blood lymphocytes when phagocytic cells were removed by iron treatment. Supernatants from lymphocytes activated by anti-beta 2m only induced low DNA synthesis in enriched T or B cells. Experiments with mitomycin treated cells indicate that cooperation and close contact between T and B cells are needed for activation by IgG anti-beta 2m. T cells are needed for B cell activation by anti-beta 2m and B cells are required for T cell activation to occur.
Ringdén
O
O
Department of Clinical Immunology, Huddinge Hospital, Sweden.
D000818:Animals; D000936:Antigen-Antibody Complex; D001402:B-Lymphocytes / Q000276:immunology*; D002469:Cell Separation; D004247:DNA / Q000096:biosynthesis; D006801:Humans; D007140:Immunoglobulin Fab Fragments; D016130:Immunophenotyping; D008213:Lymphocyte Activation; D016685:Mitomycin / Q000494:pharmacology; D010586:Phagocytes / Q000166:cytology; D011817:Rabbits; D012397:Rosette Formation; D013601:T-Lymphocytes / Q000276:immunology*; D001613:beta 2-Microglobulin / Q000276:immunology*
D000936:Antigen-Antibody Complex; D007140:Immunoglobulin Fab Fragments; D001613:beta 2-Microglobulin; D016685:Mitomycin; D004247:DNA