title: Cloning and characterization of the gene encoding the iron-sulfur protein of succinate dehydrogenase from Pleurotus ostreatus.
abstract: Genomic and cDNA fragments encoding the iron-sulfur protein (Ip) subunit of dehydrogenase (EC 1.3.99.1) have been cloned from the edible basidiomycetous fungus, Pleurotus ostreatus. The gene is interrupted by five introns and is predicted to encode a polypeptide of 268 amino acid residues. Sequence comparison with the Ip subunit from other species identified three conserved cysteine-rich clusters. One of these contains a critical histidine residue implicated in carboxin sensitivity in the heterobasidiomycete Ustilago maydis.
authors.affiliation: Wood Research Institute, Kyoto University, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Irie
authors.forename: Y
authors.initials: Y
authors.lastname: Honda
authors.forename: T
authors.initials: T
authors.lastname: Matsuyama
authors.forename: T
authors.initials: T
authors.lastname: Watanabe
authors.forename: M
authors.initials: M
authors.lastname: Kuwahara
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000302:isolation & purification; D005656:Fungal Proteins / Q000235:genetics*; D007506:Iron-Sulfur Proteins / Q000235:genetics*; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D011124:Polyporaceae / Q000201:enzymology / Q000235:genetics*; D017386:Sequence Homology, Amino Acid; D013385:Succinate Dehydrogenase / Q000235:genetics*
chemical_list: D018076:DNA, Complementary; D005656:Fungal Proteins; D007506:Iron-Sulfur Proteins; D013385:Succinate Dehydrogenase