Two populations of endothelial cell antibodies cross-react with heparin.
As endothelial cells (EC) express heparin-like glycosaminoglycans, such as heparan sulfate, it was essential to investigate the relation of anti-EC antibody (AECA) to heparin reactivity. AECA were detected in 43 of 131 autoimmune sera and anti-heparin antibodies (AHA) in 25. These autoimmune reactivities were significantly associated (P corrected < 0.0005). Seven AECA-positive/AHA-positive and three AECA-negative/AHA-positive sera were affinity-purified using protein G column followed by a heparin-Sepharose column. Two populations of AECA were recovered from the second column. One was eluted with 0.4 M NaCl which bound to EC and to solid-phase heparin with low affinity, but not to soluble heparin. The second population of AECA, which was eluted with 4 M guanidine HCl/2 M NaCl, recognized EC and solid-phase heparin with high affinity, but also soluble heparin. The latter population of AECA might thus be an important cause of autoimmune vascular thrombosis in systemic diseases.
Renaudineau
Y
Y
Laboratory of Immunology, Brest University Medical School, France.
Revelen
R
R
Bordron
A
A
Mottier
D
D
Youinou
P
P
Le Corre
R
R
D000918:Antibody Specificity; D001323:Autoantibodies / Q000032:analysis / Q000145:classification / Q000276:immunology*; D001667:Binding, Competitive / Q000276:immunology; D015415:Biomarkers / Q000032:analysis; D003429:Cross Reactions; D004730:Endothelium, Vascular / Q000276:immunology*; D006493:Heparin / Q000276:immunology*; D006801:Humans; D007074:Immunoglobulin G / Q000032:analysis / Q000302:isolation & purification
D001323:Autoantibodies; D015415:Biomarkers; D007074:Immunoglobulin G; C087589:anti-endothelial cell antibody; D006493:Heparin