title: Molecular cloning and characterization of mouse ficolin-A.
abstract: A novel ficolin-related gene was isolated from the mouse liver lambda ZAPII cDNA library. The protein encoded by this gene consists of both collagen- and fibrinogen-like domains, which are common features of the ficolin family, and was named mouse ficolin-A. The amino acid sequence of mouse ficolin-A is 60.2, 59.8, 59.8, and 59.6% identical to those of porcine ficolin-alpha, -beta, human ficolin-1, and EBP-37/P35, respectively. Northern blot analysis showed that mRNA of mouse ficolin-A is highly expressed in liver and spleen. Immunoblot analysis using an anti-mouse ficolin-A antiserum showed that mouse ficolin-A is a plasma protein with binding activity to elastin and GlcNAc.
authors.affiliation: Department of Applied Biological Science, Faculty of Agriculture, Tokyo University of Agriculture and Technology, Japan.
authors.forename: Y
authors.initials: Y
authors.lastname: Fujimori
authors.forename: S
authors.initials: S
authors.lastname: Harumiya
authors.forename: Y
authors.initials: Y
authors.lastname: Fukumoto
authors.forename: Y
authors.initials: Y
authors.lastname: Miura
authors.forename: K
authors.initials: K
authors.lastname: Yagasaki
authors.forename: H
authors.initials: H
authors.lastname: Tachikawa
authors.forename: D
authors.initials: D
authors.lastname: Fujimoto
mesh_terms: D000117:Acetylglucosamine / Q000097:blood / Q000378:metabolism; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D001798:Blood Proteins / Q000235:genetics* / Q000378:metabolism; D002352:Carrier Proteins / Q000097:blood / Q000235:genetics* / Q000378:metabolism; D003094:Collagen / Q000235:genetics; D018076:DNA, Complementary / Q000235:genetics; D004549:Elastin / Q000097:blood / Q000378:metabolism; D005340:Fibrinogen / Q000235:genetics; D037102:Lectins; D051379:Mice; D008969:Molecular Sequence Data; D011485:Protein Binding; D011487:Protein Conformation; D017386:Sequence Homology, Amino Acid; D014018:Tissue Distribution; D000097604:Ficolins
chemical_list: D000117:Acetylglucosamine; D001798:Blood Proteins; D002352:Carrier Proteins; D003094:Collagen; D018076:DNA, Complementary; D004549:Elastin; D005340:Fibrinogen; D037102:Lectins; D000097604:Ficolins