title: Loss of merlin-p85 protein complex in NF2-related tumors.
abstract: The NF2 tumor suppressor gene product, designated merlin, belongs to the family of molecules that links membranous protein with the cytoskeleton. We have previously shown that merlin was co-immunoprecipitated with a cellular protein, p85, in cultured cell. To analyze the alteration of merlin and associated proteins in surgical specimens, we developed a new method for biotin-labeling of whole cellular proteins. Screening of tumor tissues using our method showed that none of malignant gliomas and half of the NF2-related tumors had altered p85 and merlin. Our detection method seems useful for the screening of merlin alterations in NF2-related tumors.
authors.affiliation: Department of Neurosurgery, Kumamoto University School of Medicine, 1-1-1 Honjo, Kumamoto, 860, Japan.
authors.forename: H
authors.initials: H
authors.lastname: Takeshima
authors.forename: T
authors.initials: T
authors.lastname: Nishi
authors.forename: K
authors.initials: K
authors.lastname: Yamamoto
authors.forename: T
authors.initials: T
authors.lastname: Kino
authors.forename: H
authors.initials: H
authors.lastname: Nakamura
authors.forename: H
authors.initials: H
authors.lastname: Saya
authors.forename: M
authors.initials: M
authors.lastname: Kochi
authors.forename: J I
authors.initials: JI
authors.lastname: Kuratsu
authors.forename: Y
authors.initials: Y
authors.lastname: Ushio
mesh_terms: D030161:Avian Proteins; D001932:Brain Neoplasms / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D003001:Cloning, Molecular; D003598:Cytoskeletal Proteins / Q000302:isolation & purification / Q000378:metabolism*; D016515:Genes, Neurofibromatosis 2; D005910:Glioma / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D006801:Humans; D008565:Membrane Proteins / Q000096:biosynthesis / Q000302:isolation & purification / Q000378:metabolism*; D008577:Meningeal Neoplasms / Q000235:genetics / Q000378:metabolism / Q000473:pathology; D008579:Meningioma / Q000235:genetics / Q000378:metabolism / Q000473:pathology; D009442:Neurilemmoma / Q000235:genetics / Q000378:metabolism / Q000473:pathology; D025581:Neurofibromin 2; D016133:Polymerase Chain Reaction; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis / Q000302:isolation & purification / Q000378:metabolism; D014407:Tumor Cells, Cultured
chemical_list: D030161:Avian Proteins; D003598:Cytoskeletal Proteins; D008565:Membrane Proteins; D025581:Neurofibromin 2; D011993:Recombinant Fusion Proteins; C071033:cytoskeletal p85 protein, chicken