title: The glycoprotein character of multiple forms of Aspergillus polygalacturonase.
abstract: Comparisons of known primary structures of polygalacturonases show that extent and localization of potential N-glycosylation sites differ. Some sites are similar in position and adjacent to strictly conserved residues at the potential active site. The presence of N-acetylglucosamine and mannose in the molecules of two homogeneous, major Aspergillus sp. polygalacturonase forms was confirmed by IR spectroscopy. The purification method, based on interaction of the carbohydrate part with concanavalin A immobilized on chlorotriazine bead cellulose, was optimized. Deglycosylation with N-glycosidase F under denaturating and nondenaturating conditions led to molecular mass decreases followed by complete inactivation of the polygalacturonase enzyme activity. These results show the importance of glycosylation in these protein forms, while the comparative patterns establish both variability and some similarities in overall glycosylation architectures.
authors.affiliation: Institute of Chemistry, Slovak Academy of Sciences, Bratislava.
authors.forename: E
authors.initials: E
authors.lastname: Stratilová
authors.forename: D
authors.initials: D
authors.lastname: Mislovicová
authors.forename: M
authors.initials: M
authors.lastname: Kacuráková
authors.forename: E
authors.initials: E
authors.lastname: Machová
authors.forename: N
authors.initials: N
authors.lastname: Kolarová
authors.forename: O
authors.initials: O
authors.lastname: Markovic
authors.forename: H
authors.initials: H
authors.lastname: Jörnvall
mesh_terms: D000595:Amino Acid Sequence; D001234:Aspergillus niger / Q000201:enzymology*; D002846:Chromatography, Affinity; D003208:Concanavalin A / Q000378:metabolism; D006023:Glycoproteins / Q000737:chemistry*; D006031:Glycosylation; D007527:Isoenzymes / Q000737:chemistry* / Q000302:isolation & purification / Q000378:metabolism; D011096:Polygalacturonase / Q000737:chemistry* / Q000302:isolation & purification / Q000378:metabolism; D017386:Sequence Homology, Amino Acid
chemical_list: D006023:Glycoproteins; D007527:Isoenzymes; D003208:Concanavalin A; D011096:Polygalacturonase