Molecular cloning and characterization of two novel transport proteins from rat kidney.
The recent cloning of renal transport systems for organic anions and cations (OAT1, OCT1, and OCT2) opened the possibility to search, via polymerase chain reaction (PCR) homology screening, for novel transport proteins. Two integral membrane proteins, UST1 and UST2, were cloned from rat kidney. RT-PCR revealed that UST1 is confined to the kidney whereas UST2 mRNA was detected in all tested tissues. Sequence analyses suggest that UST1 and UST2, together with four related transporters, comprise, within the major facilitator superfamily, a so far unrecognized transporter family, termed amphiphilic solute facilitator (ASF) family. Characteristic signatures for the ASF family were identified.
Schömig
E
E
Department of Pharmacology, University of Heidelberg, Germany.
Spitzenberger
F
F
Engelhardt
M
M
Martel
F
F
Ording
N
N
Gründemann
D
D
D000595:Amino Acid Sequence; D000818:Animals; D003001:Cloning, Molecular; D019143:Evolution, Molecular; D007668:Kidney / Q000737:chemistry*; D008565:Membrane Proteins / Q000737:chemistry / Q000235:genetics*; D008969:Molecular Sequence Data; D027361:Organic Anion Transporters; D027701:Organic Cation Transport Proteins; D012333:RNA, Messenger / Q000235:genetics; D051381:Rats; D017386:Sequence Homology, Amino Acid; D000074058:Solute Carrier Family 22 Member 5
D008565:Membrane Proteins; D027361:Organic Anion Transporters; D027701:Organic Cation Transport Proteins; D012333:RNA, Messenger; C111542:Slc22a25 protein, rat; C115441:Slc22a5 protein, rat; D000074058:Solute Carrier Family 22 Member 5