Kappa-B like DNA-binding activity is enhanced after spaced training that induces long-term memory in the crab Chasmagnathus.
Regulation of gene expression has been involved in long-term memory consolidation. Present results support the role of Rel/ NFkappa-B like activation in this process. In the crab Chasmagnathus, the spaced presentation of 15 or more danger stimuli induces long-term habituation (LTH), while no LTH is observed after a massed training of 600 trials. When a group trained with 30 spaced trials was compared with a passive control group and massed trained groups, a higher level of specific Rel/kappa-B like DNA-binding activity was found in brain nuclear extracts. These results strongly suggest that the enhancement of Rel/kappa-B like DNA-binding activity in the brain is specifically related to LTH formation.
Freudenthal
R
R
Facultad de Ciencias Exactas y Naturales, Departamento de Ciencias Biológicas, Universidad de Buenos Aires, Argentina.
Locatelli
F
F
Hermitte
G
G
Maldonado
H
H
Lafourcade
C
C
Delorenzi
A
A
Romano
A
A
D000818:Animals; D003386:Brachyura / Q000502:physiology*; D003213:Conditioning, Psychological / Q000502:physiology; D005786:Gene Expression Regulation / Q000502:physiology*; D006185:Habituation, Psychophysiologic / Q000235:genetics*; D008297:Male; D008568:Memory / Q000502:physiology*; D016328:NF-kappa B / Q000235:genetics / Q000378:metabolism*; D009473:Neuronal Plasticity / Q000235:genetics; D015345:Oligonucleotide Probes; D011485:Protein Binding / Q000502:physiology; D015290:Second Messenger Systems / Q000235:genetics
D016328:NF-kappa B; D015345:Oligonucleotide Probes