Nitric oxide synthase inhibitor, aminoguanidine, reduces inflammation and demyelination produced by Theiler's virus infection.
This study evaluated effects of the inducible nitric oxide synthase (iNOS) inhibitor, aminoguanidine (AG), on the neuropathology and clinical disease produced by Theiler's murine encephalomyelitis virus (TMEV) DA strain infection. Treatment with AG was started on day 7, 14, 28 or 66 post-inoculation and continued for a minimum of 21 days. Inflammation, demyelination and axonal necrosis were scored in a blinded fashion on spinal cord sections from each mouse. Reduction in inflammation, demyelination and axonal necrosis was observed in mice treated with AG. Apoptosis within the spinal cord parenchyma and perivascular cuffs was significantly decreased. AG treatment resulted in delayed onset of clinical disease.
Rose
J W
JW
Neurovirology Research Laboratory, VAMC, Salt Lake City, UT 84148, USA. jrose@howard.genetics.utah.edu
Hill
K E
KE
Wada
Y
Y
Kurtz
C I
CI
Tsunoda
I
I
Fujinami
R S
RS
Cross
A H
AH
D000818:Animals; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000494:pharmacology / Q000627:therapeutic use*; D017209:Apoptosis / Q000187:drug effects; D001369:Axons / Q000473:pathology; D053938:DNA Fragmentation; D003711:Demyelinating Diseases / Q000188:drug therapy* / Q000473:pathology / Q000821:virology; D004791:Enzyme Inhibitors / Q000494:pharmacology / Q000627:therapeutic use*; D005260:Female; D006146:Guanidines / Q000494:pharmacology / Q000627:therapeutic use*; D051379:Mice; D009336:Necrosis; D009569:Nitric Oxide / Q000502:physiology; D019001:Nitric Oxide Synthase / Q000037:antagonists & inhibitors*; D052247:Nitric Oxide Synthase Type II; D011051:Poliomyelitis / Q000188:drug therapy* / Q000473:pathology / Q000821:virology; D016037:Single-Blind Method; D013116:Spinal Cord / Q000473:pathology / Q000821:virology; D017945:Theilovirus
D000894:Anti-Inflammatory Agents, Non-Steroidal; D004791:Enzyme Inhibitors; D006146:Guanidines; D009569:Nitric Oxide; D019001:Nitric Oxide Synthase; D052247:Nitric Oxide Synthase Type II; C496317:Nos2 protein, mouse; C004479:pimagedine