title: A simple whole-blood test for detecting antibodies to human immunodeficiency virus.
abstract: We developed an immunochromatographic whole-blood test (WBT) which detects antibodies to human immunodeficiency virus types 1 and 2 (HIV-1 and HIV-2) from fingerstick blood. The sensitivity and specificity of the WBT were 99.41% (1,018 confirmed positive patients) and 99.89% (941 uninfected patients), respectively (enzyme immunoassay [EIA] on serum or plasma as a reference). WBT performance was comparable to those of licensed EIAs and Western blotting, using 18 HIV-2 sera, 23 HIV-1 seroconversion panels, and a low-titer performance panel (in lieu of whole blood).
authors.affiliation: Saliva Diagnostic Systems, Inc., Vancouver, Washington 98682-2444, USA.
authors.forename: W
authors.initials: W
authors.lastname: Schramm
authors.forename: S E
authors.initials: SE
authors.lastname: Wade
authors.forename: G
authors.initials: G
authors.lastname: Barriga Angulo
authors.forename: P
authors.initials: P
authors.lastname: Castillo Torres
authors.forename: A
authors.initials: A
authors.lastname: Burgess-Cassler
mesh_terms: D015658:HIV Infections / Q000097:blood / Q000175:diagnosis* / Q000276:immunology; D015497:HIV-1 / Q000276:immunology*; D015498:HIV-2 / Q000276:immunology*; D006801:Humans; D012698:Serologic Tests / Q000379:methods*