title: Primary effusion lymphoma containing human herpesvirus 8 DNA in two AIDS patients with Kaposi's sarcoma.
abstract: OBJECTIVE
  Primary effusion lymphomas (PELs) containing Kaposi's sarcoma-associated herpesvirus (human herpesvirus 8/HHV-8) DNA sequences represent a distinct but heterogeneous group of rare non-Hodgkin's lymphomas of null-cell phenotype/B-cell origin. We aimed to describe the clinicopathologic features of two human immunodeficiency virus (HIV)-related PELs occurring in homosexual men with Kaposi's sarcoma (KS).
  
  
  METHODS
  Thoracentesis was followed by morphologic plus immunophenotypic studies and molecular analysis of tumor cell DNA by means of combination of polymerase chain reaction and Southern blot analysis.
  
  
  RESULTS
  Patients developed recurrent lymphomatous effusions lacking tissue involvement, in the context of severe immunodepression (CD4 count < 60/microL) and anti-retroviral therapy. The effusions disclosed an immunoblast-like population CD45/CD30+, but B-cell- and T-cell-associated antigen negative, showing clonal immunoglobulin heavy chain gene rearrangements and harbouring HHV-8 DNA sequences. One case contained Epstein-Barr virus genome with no evidence of c-myc, bcl-2 and bcl-6 gene alterations. Both patients had aggressive disease.
  
  
  CONCLUSIONS
  These cases represent additional examples of PEL associated with HHV-8 and confirm that the group of HIV-positive homosexual men may be at highest risk for PEL.
authors.affiliation: Dipartimento di Medicina Sperimentale e Patologia, Università La Sapienza, Rome, Italy.
authors.forename: V
authors.initials: V
authors.lastname: Ascoli
authors.forename: C M
authors.initials: CM
authors.lastname: Mastroianni
authors.forename: V
authors.initials: V
authors.lastname: Galati
authors.forename: M C
authors.initials: MC
authors.lastname: Sirianni
authors.forename: A
authors.initials: A
authors.lastname: Fruscalzo
authors.forename: A
authors.initials: A
authors.lastname: Pistilli
authors.forename: F
authors.initials: F
authors.lastname: Lo Coco
mesh_terms: D017088:AIDS-Related Opportunistic Infections / Q000150:complications / Q000821:virology*; D000328:Adult; D004279:DNA, Viral / Q000032:analysis*; D019288:Herpesvirus 8, Human / Q000235:genetics*; D006716:Homosexuality; D006801:Humans; D016483:Lymphoma, AIDS-Related / Q000150:complications / Q000821:virology*; D008228:Lymphoma, Non-Hodgkin / Q000150:complications / Q000821:virology; D008297:Male; D008875:Middle Aged; D016066:Pleural Effusion, Malignant / Q000150:complications / Q000821:virology; D012514:Sarcoma, Kaposi / Q000150:complications / Q000821:virology*
chemical_list: D004279:DNA, Viral