Simultaneous determination of cysteine sulfinic acid and hypotaurine in rat tissues by column-switching high-performance liquid chromatography with electrochemical detection.
Cysteine sulfinic acid (CSA) and hypotaurine (HT) were determined by electrochemical detection with a glassy carbon electrode at 0.95 V vs. Ag/AgCl. The separation of CSA and HT was accomplished by coupled-column liquid chromatography, consisting of an anion-exchange column and a cation-exchange column. For the determination of CSA and HT in rat tissues, a column-switching system was introduced to remove interferences from late-eluting endogenous substances. The limits of determination were 0.05 microM for both sulfinic acids. The average precisions (C.V.) over the concentration range of 0.05 to 5 microM were 4.3% for CSA and 4.2% for HT.
Togawa
T
T
Department of Analytical Chemistry, Meiji College of Pharmacy, Tokyo, Japan.
Ohsawa
A
A
Kawanabe
K
K
Tanabe
S
S
D000818:Animals; D001923:Brain Chemistry; D002244:Carbon; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D003545:Cysteine / Q000031:analogs & derivatives* / Q000032:analysis / Q000302:isolation & purification; D004563:Electrochemistry; D004566:Electrodes; D007668:Kidney / Q000737:chemistry; D008099:Liver / Q000737:chemistry; D008297:Male; D018377:Neurotransmitter Agents; D051381:Rats; D017208:Rats, Wistar; D013654:Taurine / Q000031:analogs & derivatives* / Q000032:analysis / Q000302:isolation & purification
D018377:Neurotransmitter Agents; D013654:Taurine; C003949:hypotaurine; D002244:Carbon; D003545:Cysteine; C013461:cysteine sulfinic acid