title: Transactivation potential of the C-terminus of human Nm23-H1.
abstract: The transactivation potential of Nm23-H1, a homolog of c-myc transcription factor Nm23-H2/PuF was assessed in yeast as a fusion protein with the DNA binding domains (DBDs) of GAL4 and LexA. The C-terminal half of Nm23-H1 exhibited strong transactivation of the reporter genes, LacZ and Leu2 carrying GAL4 and LexA upstream activating sequences (UASs), whereas the full-length Nm23-H1 and its N-terminal did not. Similar results were also obtained with Nm23-H2/PuF transactivating the reporter genes only by the C-terminus fused to GAL4 and LexA DBDs. Hence, our results suggested a possible regulatory role of the N-termini of Nm23 isotypes upon transactivation.
authors.affiliation: Division of Life Sciences, PaiChai University, Taejon, South Korea.
authors.forename: S K
authors.initials: SK
authors.lastname: Chae
authors.forename: N S
authors.initials: NS
authors.lastname: Lee
authors.forename: K J
authors.initials: KJ
authors.lastname: Lee
authors.forename: E
authors.initials: E
authors.lastname: Kim
mesh_terms: D001426:Bacterial Proteins / Q000235:genetics; D004268:DNA-Binding Proteins; D005786:Gene Expression Regulation; D017930:Genes, Reporter / Q000235:genetics; D006801:Humans; D007763:Lac Operon / Q000235:genetics; D020559:Monomeric GTP-Binding Proteins; D054778:NM23 Nucleoside Diphosphate Kinases; D009701:Nucleoside-Diphosphate Kinase; D011506:Proteins / Q000235:genetics; D062085:RNA, Long Noncoding; D011993:Recombinant Fusion Proteins / Q000235:genetics; D029701:Saccharomyces cerevisiae Proteins; D012697:Serine Endopeptidases / Q000235:genetics; D014157:Transcription Factors / Q000235:genetics* / Q000378:metabolism; D015533:Transcriptional Activation / Q000235:genetics*; D014166:Transferases; D025521:Tumor Suppressor Proteins; D000099160:LexA Repressor Protein
chemical_list: D001426:Bacterial Proteins; D004268:DNA-Binding Proteins; D020559:Monomeric GTP-Binding Proteins; D054778:NM23 Nucleoside Diphosphate Kinases; D009701:Nucleoside-Diphosphate Kinase; D011506:Proteins; D062085:RNA, Long Noncoding; D011993:Recombinant Fusion Proteins; D029701:Saccharomyces cerevisiae Proteins; D012697:Serine Endopeptidases; D014157:Transcription Factors; D014166:Transferases; D025521:Tumor Suppressor Proteins; D000099160:LexA Repressor Protein; C109420:DLEU2 lncRNA, human; C052130:GAL4 protein, S cerevisiae; C517938:NME1 protein, human