title: Characterization of ARC, a divergent member of the AAA ATPase family from Rhodococcus erythropolis.
abstract: A gene encoding a AAA ATPase was discovered in the 5' region of the second operon of 20 S proteasome subunits in the nocardioform actinomycete Rhodococcus erythropolis NI86/21. The gene was cloned and expressed in Escherichia coli. The protein, ARC (AAA ATPase forming Ring-shaped Complexes), is a divergent member of the AAA family. The deduced product of the arc gene is 591 residues long (66 kDa). The purified protein possesses a low, N-ethylmaleimide-sensitive ATPase activity and forms rings of six subunits, arranged symmetrically around a central opening or cavity. Two-dimensional crystals grown on lipid monolayers yielded images of the ATPase molecules in "end-on" orientation at 1.9 nm resolution.
authors.affiliation: Max-Planck-Institut für Biochemie, Am Klopferspitz 18a, Martinsried, D-82152, Germany.
authors.forename: S
authors.initials: S
authors.lastname: Wolf
authors.forename: I
authors.initials: I
authors.lastname: Nagy
authors.forename: A
authors.initials: A
authors.lastname: Lupas
authors.forename: G
authors.initials: G
authors.lastname: Pfeifer
authors.forename: Z
authors.initials: Z
authors.lastname: Cejka
authors.forename: S A
authors.initials: SA
authors.lastname: Müller
authors.forename: A
authors.initials: A
authors.lastname: Engel
authors.forename: R
authors.initials: R
authors.lastname: De Mot
authors.forename: W
authors.initials: W
authors.lastname: Baumeister
mesh_terms: D000595:Amino Acid Sequence; D001425:Bacterial Outer Membrane Proteins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D001665:Binding Sites; D003001:Cloning, Molecular; D004926:Escherichia coli / Q000235:genetics; D029968:Escherichia coli Proteins; D008854:Microscopy, Electron / Q000379:methods; D008969:Molecular Sequence Data; D009711:Nucleotides / Q000378:metabolism; D011487:Protein Conformation; D011994:Recombinant Proteins / Q000737:chemistry / Q000235:genetics; D012097:Repressor Proteins; D012240:Rhodococcus / Q000201:enzymology*; D017386:Sequence Homology, Amino Acid
chemical_list: D001425:Bacterial Outer Membrane Proteins; D029968:Escherichia coli Proteins; D009711:Nucleotides; D011994:Recombinant Proteins; D012097:Repressor Proteins; C041610:arcA protein, E coli