Surface-exposed phenylalanines in the RNP1/RNP2 motif stabilize the cold-shock protein CspB from Bacillus subtilis.
In the cold-shock protein CspB from Bacillus subtilis three exposed Phe residues (F15, F17, and F27) are essential for its function in binding to single-stranded nucleic acids. Usually, the hydrophobic Phe side chains are buried in folded proteins. We asked here whether the exposition of the essential Phe residues could be a cause for the very low conformational stability of CspB. Urea-induced and heat-induced equilibrium unfolding transitions were measured for three mutants of CspB, where Phe 15, Phe 17, and Phe 27 were individually replaced by alanine. Unexpectedly, all three mutations strongly destabilized CspB. The aromatic side chains of Phe 15, Phe 17, and Phe 27 in the active site are thus important for both binding to nucleic acids and conformational stability. There is no compromise between function and stability in the active site. Model calculations indicate that, although they are partially exposed to solvent, all three Phe residues nevertheless lose accessible surface upon folding, and this should favor the native state. A different result is obtained with the F38A variant. Phe 38 is hyperexposed in native CspB, and its substitution by Ala is in fact stabilizing.
Schindler
T
T
Laboratorium für Biochemie, Universität Bayreuth, Germany.
Perl
D
D
Graumann
P
P
Sieber
V
V
Marahiel
M A
MA
Schmid
F X
FX
D001412:Bacillus subtilis / Q000737:chemistry*; D001426:Bacterial Proteins / Q000737:chemistry; D002352:Carrier Proteins / Q000737:chemistry* / Q000235:genetics; D006360:Heat-Shock Proteins; D009154:Mutation; D009696:Nucleic Acids / Q000737:chemistry; D010649:Phenylalanine / Q000737:chemistry*; D011485:Protein Binding; D011487:Protein Conformation; D016601:RNA-Binding Proteins / Q000737:chemistry*; D012261:Ribonucleoproteins; D012269:Ribosomal Proteins / Q000737:chemistry*; D029701:Saccharomyces cerevisiae Proteins
D001426:Bacterial Proteins; D002352:Carrier Proteins; D006360:Heat-Shock Proteins; D009696:Nucleic Acids; D016601:RNA-Binding Proteins; C082384:RNP1 protein, S cerevisiae; D012261:Ribonucleoproteins; D012269:Ribosomal Proteins; D029701:Saccharomyces cerevisiae Proteins; C077188:cold-shock protein CspB, Bacteria; D010649:Phenylalanine