title: Hydrogen peroxide inhibits giant cell tumor and osteoblast metabolism in vitro.
abstract: This study investigates the efficacy of using hydrogen peroxide as adjuvant therapy after extended local curettage for benign giant cell tumors of bone. Hydrogen peroxide is used clinically as a chemical adjuvant for removal of residual tumor cells, presumably by effervescent cleansing with minimal damage to surrounding soft tissue and bone cells. This investigation examined the effects of hydrogen peroxide on giant cell tumor cells and osteoblasts grown in culture. Fresh fragments of histologically confirmed giant cell tumor tissue (six patients) and trabecular bone (one patient) were excised. Cells obtained from the fragments were grown in culture. Confluent cell cultures were exposed to saline (control) or hydrogen peroxide (0.1-1000 mm) for 2 minutes, and incubation was continued for 12, 24, or 48 hours without hydrogen peroxide. Protein content, deoxyribonucleic acid content, tartrate resistant acid phosphatase activity, and alkaline phosphatase activity were measured in the cell layers. The medium from the final 12 hours of each incubation period was used to evaluate lactate production. Cell lysis or death occurred after exposing giant cell tumor cells and osteoblasts to 100 mm and 30 mm hydrogen peroxide, respectively, concentrations substantially lower than the 3% (880 mm) hydrogen peroxide commonly used clinically. These results support the theory of using a minimal concentration of hydrogen peroxide as a chemical adjuvant in the surgical treatment of giant cell tumors of bone.
authors.affiliation: Department of Orthopaedic Surgery, Carolinas Medical Center, Charlotte, NC 28232-2861, USA.
authors.forename: N C
authors.initials: NC
authors.lastname: Nicholson
authors.forename: W K
authors.initials: WK
authors.lastname: Ramp
authors.forename: J S
authors.initials: JS
authors.lastname: Kneisl
authors.forename: K K
authors.initials: KK
authors.lastname: Kaysinger
mesh_terms: D000328:Adult; D000890:Anti-Infective Agents / Q000494:pharmacology* / Q000627:therapeutic use; D001859:Bone Neoplasms / Q000188:drug therapy / Q000378:metabolism* / Q000601:surgery; D017024:Chemotherapy, Adjuvant; D005260:Female; D018212:Giant Cell Tumor of Bone / Q000188:drug therapy / Q000378:metabolism* / Q000601:surgery; D006801:Humans; D006861:Hydrogen Peroxide / Q000494:pharmacology* / Q000627:therapeutic use; D008297:Male; D010006:Osteoblasts / Q000187:drug effects / Q000378:metabolism*; D014407:Tumor Cells, Cultured
chemical_list: D000890:Anti-Infective Agents; D006861:Hydrogen Peroxide