Interactions of arbutin with dry and hydrated bilayers.
The glycosylated hydroquinone arbutin (4-hydroxyphenyl-beta-D-glucopyranoside) is abundant in certain resurrection plants, which can survive almost complete dehydration for prolonged periods. Little is known about the role of arbutin in vivo, but it is thought to contribute toward survival of the plants in the dry state. We have investigated the interactions of arbutin with model membranes under conditions of high and low hydration, as well as the possible participation of arbutin in carbohydrate glasses formed at low water contents. Retention of a trapped soluble marker inside large unilamellar vesicles and fusion of vesicles was monitored by fluorescence spectroscopy. Effects of arbutin on glass-transition temperatures and hydrated membrane phase-transition temperatures were measured by differential scanning calorimetry. The possible insertion of arbutin into membrane bilayers was estimated by following arbutin auto-fluorescence. Evidence is presented that arbutin does not change the glass-transition temperature of a sucrose/trehalose glass, but that arbutin does interact with hydrated membranes by insertion of the phenol moiety into the lipid bilayer. This interaction causes increased membrane leakage during air-drying by a mechanism other than vesicle-vesicle fusion. Implications of these effects on the dehydrated plant cells, as well as possible methods of obviating the damage, are discussed.
Oliver
A E
AE
Section of Molecular and Cellular Biology, University of California, Davis, CA 95616, USA. aeoliver@ucdavis.edu
Hincha
D K
DK
Crowe
L M
LM
Crowe
J H
JH
D015060:1,2-Dipalmitoylphosphatidylcholine / Q000737:chemistry; D001104:Arbutin / Q000737:chemistry*; D002152:Calorimetry, Differential Scanning; D002241:Carbohydrates / Q000737:chemistry; D003890:Desiccation; D004134:Dimyristoylphosphatidylcholine / Q000737:chemistry; D004305:Dose-Response Relationship, Drug; D005453:Fluorescence; D008051:Lipid Bilayers / Q000737:chemistry*; D010713:Phosphatidylcholines / Q000737:chemistry; D013696:Temperature; D014867:Water / Q000737:chemistry*
D002241:Carbohydrates; D008051:Lipid Bilayers; D010713:Phosphatidylcholines; D014867:Water; D015060:1,2-Dipalmitoylphosphatidylcholine; D001104:Arbutin; C017251:1,2-oleoylphosphatidylcholine; C028694:1-palmitoyl-2-oleoylphosphatidylcholine; D004134:Dimyristoylphosphatidylcholine