title: Role of DNA in the activation of the Cry1A insecticidal crystal protein from Bacillus thuringiensis.
abstract: The Cry1A insecticidal crystal protein (protoxin) from six subspecies of Bacillus thuringiensis as well as the Cry1Aa, Cry1Ab, and Cry1Ac proteins cloned in Escherichia coli was found to contain 20-kilobase pair DNA. Only the N-terminal toxic moiety of the protoxin was found to interact with the DNA. Analysis of the crystal gave approximately 3 base pairs of DNA per molecule of protoxin, indicating that only a small region of the N-terminal toxic moiety interacts with the DNA. It is proposed that the DNA-protoxin complex is virus-like in structure with a central DNA core surrounded by protein interacting with the DNA with the peripheral ends of the C-terminal region extending outward. It is shown that this structure accounts for the unusual proteolysis observed in the generation of toxin in which it appears that peptides are removed by obligatory sequential cleavages starting from the C terminus of the protoxin. Activation of the protoxin by spruce budworm (Choristoneura fumiferana) gut juice is shown to proceed through intermediates consisting of protein-DNA complexes. Larval trypsin initially converts the 20-kilobase pair DNA-protoxin complex to a 20-kilobase pair DNA-toxin complex, which is subsequently converted to a 100-base pair DNA-toxin complex by a gut nuclease and ultimately to the DNA-free toxin.
authors.affiliation: Department of Chemistry, University of Ottawa, Ottawa, Ontario K1N 6N5.
authors.forename: F R
authors.initials: FR
authors.lastname: Clairmont
authors.forename: R E
authors.initials: RE
authors.lastname: Milne
authors.forename: V T
authors.initials: VT
authors.lastname: Pham
authors.forename: M B
authors.initials: MB
authors.lastname: Carrière
authors.forename: H
authors.initials: H
authors.lastname: Kaplan
mesh_terms: D000818:Animals; D001413:Bacillus thuringiensis / Q000502:physiology*; D000083722:Bacillus thuringiensis Toxins; D001426:Bacterial Proteins / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism*; D001427:Bacterial Toxins / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism*; D003001:Cloning, Molecular; D004269:DNA, Bacterial / Q000302:isolation & purification / Q000378:metabolism*; D004068:Digestive System Physiological Phenomena; D004731:Endotoxins / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism*; D004926:Escherichia coli; D006460:Hemolysin Proteins; D007306:Insecticides / Q000302:isolation & purification / Q000378:metabolism; D008958:Models, Molecular; D009036:Moths / Q000502:physiology*; D009690:Nucleic Acid Conformation; D011485:Protein Binding; D011487:Protein Conformation; D011498:Protein Precursors / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism*; D011994:Recombinant Proteins / Q000302:isolation & purification / Q000378:metabolism
chemical_list: D000083722:Bacillus thuringiensis Toxins; C029103:Bacillus thuringiensis protoxin; D001426:Bacterial Proteins; D001427:Bacterial Toxins; D004269:DNA, Bacterial; D004731:Endotoxins; D006460:Hemolysin Proteins; D007306:Insecticides; D011498:Protein Precursors; D011994:Recombinant Proteins; C030956:insecticidal crystal protein, Bacillus Thuringiensis