title: Improved high-performance liquid chromatographic separation of peptidoglycan isolated from various Staphylococcus aureus strains for mass spectrometric characterization.
abstract: Reversed-phase high-performance liquid chromatography (RP-HPLC) of muropeptides, obtained by muramidase digestion of peptidoglycan in combination with amino acid analysis and plasma desorption time-of-flight mass spectrometry is today by far the best tool to analyze the fine structure of the peptidoglycans. Here we report further improvements of the RP-HPLC separation of muropeptides for analyzing the peptidoglycans of various methicillin-resistant strains of Staphylococcus aureus, with emphasis on a more detailed characterization of the interpeptide bridge of the peptidoglycans of this species.
authors.affiliation: Robert Koch Institute, Berlin, Germany.
authors.forename: M
authors.initials: M
authors.lastname: Roos
authors.forename: E
authors.initials: E
authors.lastname: Pittenauer
authors.forename: E
authors.initials: E
authors.lastname: Schmid
authors.forename: M
authors.initials: M
authors.lastname: Beyer
authors.forename: B
authors.initials: B
authors.lastname: Reinike
authors.forename: G
authors.initials: G
authors.lastname: Allmaier
authors.forename: H
authors.initials: H
authors.lastname: Labischinski
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D013058:Mass Spectrometry / Q000379:methods; D010457:Peptidoglycan / Q000737:chemistry / Q000302:isolation & purification*; D015203:Reproducibility of Results; D013211:Staphylococcus aureus / Q000737:chemistry*; D013696:Temperature
chemical_list: D010457:Peptidoglycan