In vitro effects of endothelin-1 on somatostatin and thyrotropin-releasing hormone release from the rat stomach.
The effects of endothelin (ET) 1 on the release of somatostatin (SS) and thyrotropin-releasing hormone (TRH) from the rat stomach were studied in vitro. The rat stomach was incubated in medium 199 with 1.0 mg/ml of bacitracin (pH 7.4) for 20 min. The amounts of SS and TRH released into the medium were measured by individual radioimmunoassays. With the addition of ET-1, the release of SS from the rat stomach was inhibited significantly in a dose-related manner, whereas TRH released from the stomach was enhanced significantly. These effects of ET-1 on SS or TRH release were blocked by BQ-485, a blocker of ETA receptor. These findings suggest that ET-1 inhibits SS and stimulates TRH release from the rat stomach in vitro, and that these effects are mediate via ETA receptor.
Mitsuma
T
T
The Fourth Department of Internal Medicine, Aichi Medical University, Aichi, Japan.
Kaneko
H
H
Hirooka
Y
Y
Mori
S
S
Mori
Y
Y
Adachi
K
K
Yamamoto
S
S
Hayakawa
T
T
Yamaguchi
C
C
Uruma
M
M
Rhue
N
N
Ping
J
J
Nogimori
T
T
D000818:Animals; D001381:Azepines / Q000494:pharmacology; D065128:Endothelin Receptor Antagonists; D019332:Endothelin-1 / Q000037:antagonists & inhibitors / Q000494:pharmacology*; D005753:Gastric Mucosa / Q000378:metabolism; D066298:In Vitro Techniques; D008297:Male; D009842:Oligopeptides / Q000494:pharmacology; D011863:Radioimmunoassay; D051381:Rats; D017208:Rats, Wistar; D044022:Receptor, Endothelin A; D013004:Somatostatin / Q000378:metabolism*; D013270:Stomach / Q000187:drug effects*; D013973:Thyrotropin-Releasing Hormone / Q000378:metabolism*
D001381:Azepines; D065128:Endothelin Receptor Antagonists; D019332:Endothelin-1; D009842:Oligopeptides; D044022:Receptor, Endothelin A; C083116:BQ 485; D013004:Somatostatin; D013973:Thyrotropin-Releasing Hormone