FacB, the Aspergillus nidulans activator of acetate utilization genes, binds dissimilar DNA sequences.
The facB gene is required for acetate induction of acetamidase (amdS) and the acetate utilization enzymes acetyl-CoA synthase (facA), isocitrate lyase (acuD) and malate synthase (acuE) in Aspergillus nidulans. The facB gene encodes a transcriptional activator with a GAL4-type Zn(II)2Cys6 zinc binuclear cluster DNA-binding domain which is shown to be required for DNA binding. In vitro DNA-binding sites for FacB in the 5' regions of the amdS, facA, acuD and acuE genes have been identified. Mutations in amdS FacB DNA-binding sites affected expression of an amdS-lacZ reporter in vivo and altered the affinity of in vitro DNA binding. This study shows that the FacB Zn(II)2Cys6 cluster binds to dissimilar sites which show similarity in form but not sequence with DNA-binding sites of other Zn(II)2Cys6 proteins. Sequences with homology to FacB sites are found in the 5' regions of genes regulated by the closely related yeast Zn(II)2Cys6 protein CAT8.
Todd
R B
RB
Department of Genetics, The University of Melbourne, Parkville, Victoria 3052, Australia.
Andrianopoulos
A
A
Davis
M A
MA
Hynes
M J
MJ
D000085:Acetates / Q000378:metabolism*; D000581:Amidohydrolases / Q000235:genetics; D001233:Aspergillus nidulans / Q000235:genetics*; D001483:Base Sequence; D001665:Binding Sites; D018983:DNA Footprinting; D004271:DNA, Fungal / Q000235:genetics / Q000378:metabolism*; D005656:Fungal Proteins; D015966:Gene Expression Regulation, Fungal / Q000235:genetics; D005800:Genes, Fungal; D009154:Mutation; D011401:Promoter Regions, Genetic / Q000235:genetics; D011993:Recombinant Fusion Proteins; D012689:Sequence Homology, Nucleic Acid; D015534:Trans-Activators / Q000235:genetics / Q000378:metabolism*; D015032:Zinc / Q000737:chemistry*
D000085:Acetates; D004271:DNA, Fungal; D005656:Fungal Proteins; D011993:Recombinant Fusion Proteins; D015534:Trans-Activators; D000581:Amidohydrolases; C021445:acetamidase; D015032:Zinc