Adherence to and penetration of human intestinal Caco-2 epithelial cell monolayers by Pseudomonas aeruginosa.
Clinical isolates of Pseudomonas aeruginosa from blood adhered to and penetrated intestinal Caco-2 cell monolayers to a greater degree than did isolates from sputum, with a concomitant drastic decrease in transepithelial electrical resistance. PAO-PR1, an avirulent exotoxin A mutant of PAO1, did not cause a decrease in the resistance. The Caco-2 monolayer system may be useful for the evaluation of certain P. aeruginosa virulence factor activities.
Hirakata
Y
Y
Department of Laboratory Medicine, Nagasaki University School of Medicine, Sakamoto, Japan. hirakata@net.nagasaki-u.ac.jp
Izumikawa
K
K
Yamaguchi
T
T
Igimi
S
S
Furuya
N
N
Maesaki
S
S
Tomono
K
K
Yamada
Y
Y
Kohno
S
S
Yamaguchi
K
K
Kamihira
S
S
D001422:Bacterial Adhesion; D018938:Caco-2 Cells; D017097:Electric Impedance; D006801:Humans; D011550:Pseudomonas aeruginosa / Q000502:physiology*; D014774:Virulence