title: Activation of a plant plasma membrane Ca2+ channel by TGalpha1, a heterotrimeric G protein alpha-subunit homologue.
abstract: Wild-type and GTPase-deficient recombinant TGalpha1 were used along patch-clamp techniques to study the role of heterotrimeric G proteins in the regulation of the hyperpolarized active tomato plasma membrane Ca2+ channel. Recombinant alpha-subunits induced an increase in channel activity as shown by the increase in channel events and the mean open probability of the channel. Our results suggest a membrane-delimited pathway involving heterotrimeric G proteins in Ca2+ channel activation.
authors.affiliation: Department of Botany, University of Toronto, Ont., Canada.
authors.forename: G S
authors.initials: GS
authors.lastname: Aharon
authors.forename: A
authors.initials: A
authors.lastname: Gelli
authors.forename: W A
authors.initials: WA
authors.lastname: Snedden
authors.forename: E
authors.initials: E
authors.lastname: Blumwald
mesh_terms: D000595:Amino Acid Sequence; D015220:Calcium Channels / Q000378:metabolism*; D002462:Cell Membrane / Q000502:physiology; D019204:GTP-Binding Proteins / Q000378:metabolism*; D006160:Guanosine Triphosphate / Q000378:metabolism; D018551:Solanum lycopersicum; D008969:Molecular Sequence Data; D010940:Plant Proteins / Q000378:metabolism*; D011994:Recombinant Proteins / Q000276:immunology / Q000378:metabolism; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid; D015398:Signal Transduction
chemical_list: D015220:Calcium Channels; D010940:Plant Proteins; D011994:Recombinant Proteins; D006160:Guanosine Triphosphate; D019204:GTP-Binding Proteins