Local changes in GTP-binding protein immunoreactivities in human epileptogenic neocortex.
The relative levels of guanine nucleotide-binding protein alpha-subunits Gi1alpha, Gi2alpha, Gi3alpha, Go(alpha), Gs(alpha), and Gx/z(alpha) were measured in neocortex removed at surgery from patients with intractable temporal lobe epilepsy. Immunoreactivity was quantified using specific polyclonal antisera against the Galpha-subunits according to the Laurell "rocket" immunoelectrophoresis technique. We compared the G protein contents of spiking (active) and nonspiking (nonactive) cortical regions, based on intraoperative electrocorticography, within the same and different patients. There were no clear trends for lower or higher levels of G-protein subtypes to be found in the samples of protein extracts from nonspiking regions as compared to spiking regions. However, comparison of paired samples of spiking and nonspiking cortex within the same patient demonstrated that levels of certain G-protein subtypes were either increased or decreased in all patients. This indicates that cortical regions with enhanced neuronal activity may produce microzonal alterations in the levels of G proteins. Moreover, our results suggest that high levels of Gi1alpha and low levels of the other G-protein subtypes appear to be associated with a greater susceptibility to maintaining spiking activity.
DeFelipe
J
J
Instituto Cajal (CSIC), Madrid, Spain. defelipe@cajal.csic.es
Marco
P
P
Sola
R G
RG
Sánchez-Blázquez
P
P
Garzón
J
J
D000328:Adult; D002540:Cerebral Cortex / Q000378:metabolism / Q000502:physiology*; D004569:Electroencephalography; D004827:Epilepsy / Q000209:etiology*; D005260:Female; D019204:GTP-Binding Proteins / Q000378:metabolism*; D006801:Humans; D007122:Immunoelectrophoresis; D008297:Male
D019204:GTP-Binding Proteins