Differential expression of cytochrome P4502E1 (CYP2E1) in Morris hepatomas and livers of tumor bearing rats.
The objective of this investigation was to determine if the decrease of cytochrome P4502E1 (CYP2E1) in hepatomas is related to the tumor growth rate. There was a significant correlation between N-nitrosodimethylamine (NDMA) demethylase activities and CYP2E1 protein levels in rat liver and hepatomas. The levels of NDMA demethylase activities and CYP2E1 protein content were lower in hepatomas than in the corresponding host livers. NDMA demethylase activities and CYP2E1 protein levels were greater in hepatomas of slow and intermediate growth rate than in fast growing hepatomas. A similar trend was also observed with CYP2E1 mRNA levels. The results demonstrated an inverse relationship between growth rate of rat hepatomas and the expression of CYP2E1.
Hu
J J
JJ
Georgetown University Medical Center, Lombardi Cancer Center and Department of Pharmacology, The Research Building, E-316, 3970 Reservoir Rd., Washington, DC 20007, USA.
Hong
J Y
JY
Lea
M A
MA
Yang
C S
CS
D000096:Acetone / Q000494:pharmacology; D000818:Animals; D002455:Cell Division; D019392:Cytochrome P-450 CYP2E1 / Q000032:analysis / Q000096:biosynthesis*; D015342:DNA Probes; D005215:Fasting; D015971:Gene Expression Regulation, Enzymologic / Q000187:drug effects; D015972:Gene Expression Regulation, Neoplastic; D007700:Kinetics; D008114:Liver Neoplasms, Experimental / Q000201:enzymology* / Q000473:pathology*; D008297:Male; D008861:Microsomes / Q000201:enzymology*; D008862:Microsomes, Liver / Q000201:enzymology*; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D011915:Rats, Inbred BUF; D012016:Reference Values
D015342:DNA Probes; D012333:RNA, Messenger; D000096:Acetone; D019392:Cytochrome P-450 CYP2E1