title: Isolation and genomic characterization of the TUPLE1/HIRA gene of the pufferfish Fugu rubripes.
abstract: In an effort to obtain a small genomic construct for the generation of a HIRA transgenic mouse, we have isolated and sequenced the Fugu TUPLE1/HIRA gene. We have compared the gene organization and the proteins encoded in pufferfish and human and also searched for conserved DNA sequences that might be important in gene regulation. The pufferfish gene spans approx. 9 kb, which is approx. 11 times smaller than the human gene, owing to the reduced size of the introns. Like its human counterpart, it is organized into 25 exons. The majority of the splice sites are in identical positions to those found in the human gene, however, for three internal exons the positions of the splice sites are not directly comparable. The coding regions are almost identical in size and show a high degree of similarity, especially at the amino and carboxy termini. Comparisons of 5' and 3' sequences failed to detect similarities or sequences involved in regulation.
authors.affiliation: Cancer Research Institute, Hospital Duran i Reynals, Molecular Genetics Department, Barcelona, Spain.
authors.forename: R
authors.initials: R
authors.lastname: Llevadot
authors.forename: X
authors.initials: X
authors.lastname: Estivill
authors.forename: P
authors.initials: P
authors.lastname: Scambler
authors.forename: M
authors.initials: M
authors.lastname: Pritchard
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D018797:Cell Cycle Proteins; D002645:Chickens; D003062:Codon; D017124:Conserved Sequence; D017931:DNA Primers; D005400:Fishes, Poisonous / Q000235:genetics*; D005786:Gene Expression Regulation; D016678:Genome; D056488:Histone Chaperones; D006801:Humans; D051379:Mice; D008822:Mice, Transgenic; D008969:Molecular Sequence Data; D009687:Nuclear Proteins / Q000096:biosynthesis / Q000737:chemistry / Q000235:genetics*; D016133:Polymerase Chain Reaction; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid; D014157:Transcription Factors / Q000096:biosynthesis / Q000737:chemistry / Q000235:genetics*
chemical_list: D018797:Cell Cycle Proteins; D003062:Codon; D017931:DNA Primers; C086057:HIRA protein, human; C484187:Hira protein, mouse; D056488:Histone Chaperones; D009687:Nuclear Proteins; D014157:Transcription Factors