title: A high-resolution metric HAPPY map of human chromosome 14.
abstract: We have mapped 1001 novel sequence-tagged sites on human chromosome 14. The mean spacing between markers is approximately 90 kb, most markers are mapped with a resolution of better than 100 kb, and physical distances are determined. The map was produced using HAPPY mapping, a simple and widely applicable in vitro approach that is analogous to linkage or to radiation hybrid mapping, but that circumvents many of the difficulties and potential artifacts associated with these methods. We show also that the map serves as a robust scaffold for building physical maps using large-insert clones.
authors.affiliation: Medical Research Council Laboratory of Molecular Biology, Cambridge, United Kingdom. phd@mrc-lmb.cam.ac.uk
authors.forename: P H
authors.initials: PH
authors.lastname: Dear
authors.forename: A T
authors.initials: AT
authors.lastname: Bankier
authors.forename: M B
authors.initials: MB
authors.lastname: Piper
mesh_terms: D000818:Animals; D002460:Cell Line; D002874:Chromosome Mapping / Q000379:methods*; D002883:Chromosomes, Human, Pair 14 / Q000235:genetics*; D006224:Cricetinae; D008040:Genetic Linkage; D005819:Genetic Markers / Q000235:genetics; D006801:Humans; D051379:Mice; D016324:Sequence Tagged Sites
chemical_list: D005819:Genetic Markers