title: Dendritic cells fused with mastocytoma cells elicit therapeutic antitumor immunity.
abstract: Characterization of the spontaneous immune response that frequently occurs in tumor-bearing animals, as well as immunization using dendritic cells pulsed with tumor antigens, suggests that a limiting factor of the tumor-specific immune response may be a defect in the co-stimulatory signal that is required for optimal activation of T cells. In this work, we describe a new approach to improve the antigen-presenting capacity of tumor cells, which does not require a source of purified tumor-associated antigen. We fused P815 mastocytoma cells with bone marrow-derived dendritic cells. We obtained one hybrid that displayed the phenotypic and functional properties of dendritic cells and expressed mRNA coding for the tumor-associated antigen P815 A/B. Injections of irradiated hybrid cells prevented the growth of preimplanted mastocytoma and induced long-lasting tumor resistance.
authors.affiliation: Départment of Molecular Biology, Université Libre de Bruxelles, Rhode-Saint-Genèse, Belgium.
authors.forename: L
authors.initials: L
authors.lastname: Lespagnard
authors.forename: P
authors.initials: P
authors.lastname: Mettens
authors.forename: A M
authors.initials: AM
authors.lastname: Verheyden
authors.forename: N
authors.initials: N
authors.lastname: Tasiaux
authors.forename: K
authors.initials: K
authors.lastname: Thielemans
authors.forename: S
authors.initials: S
authors.lastname: van Meirvenne
authors.forename: A
authors.initials: A
authors.lastname: Geldhof
authors.forename: P
authors.initials: P
authors.lastname: De Baetselier
authors.forename: J
authors.initials: J
authors.lastname: Urbain
authors.forename: O
authors.initials: O
authors.lastname: Leo
authors.forename: M
authors.initials: M
authors.lastname: Moser
mesh_terms: D000818:Animals; D001854:Bone Marrow Cells / Q000187:drug effects / Q000276:immunology; D002459:Cell Fusion; D003713:Dendritic Cells / Q000166:cytology / Q000276:immunology*; D005260:Female; D016178:Granulocyte-Macrophage Colony-Stimulating Factor / Q000494:pharmacology; D016219:Immunotherapy, Adoptive; D012515:Mast-Cell Sarcoma / Q000276:immunology* / Q000473:pathology / Q000517:prevention & control*; D051379:Mice; D008808:Mice, Inbred CBA; D008811:Mice, Inbred DBA; D010641:Phenotype; D012333:RNA, Messenger / Q000378:metabolism; D014407:Tumor Cells, Cultured; D014409:Tumor Necrosis Factor-alpha / Q000494:pharmacology
chemical_list: D012333:RNA, Messenger; D014409:Tumor Necrosis Factor-alpha; D016178:Granulocyte-Macrophage Colony-Stimulating Factor