title: Development and pharmacological characterization of a modified procedure for the measurement of carbonic anhydrase activity.
abstract: Carbonic anhydrases (CAs) are a family of zinc metalloenzymes of molecular mass 30-60 kDa; seven different isoenzymes belong to this family (Okuyama et al., 1992, Proc Natl Acad Sci USA 89:1315-1319). They may be broadly recognized according to the efficiency with which they catalyze the reversible interconversion of CO2 and HCO3-, and they differ in physicochemical properties, in sensitivity to various inhibitors and in their subcellular localization; cytoplasmic (CA I, CA II, CA III, and CA VII), cell-surface membrane (CA IV), and mitochondrial (CA V) and secretory (CA VI) isoenzymes have been described. Several methods are reported in the literature for the measure of CA enzymatic activity; they may be broadly divided into two categories: those based on the measure of pH variation (pH-stat and colorimetric assays) (Wu et al., 1993, J Ocular Pharm 9:97-108; Maren, 1991, Molec Pharmacol 41:419-426) and the ones in which CO2 production is measured through pCO2 sensors (Botrè and Botrè, 1990, Anal Biochem 185:254-264).
authors.affiliation: Angelini Ricerche, S.p.A., Immunopharmacology and Analysis Laboratory, Pomezia, Rome, Italy.
authors.forename: C
authors.initials: C
authors.lastname: Landolfi
authors.forename: M
authors.initials: M
authors.lastname: Marchetti
authors.forename: G
authors.initials: G
authors.lastname: Ciocci
authors.forename: C
authors.initials: C
authors.lastname: Milanese
mesh_terms: D000086:Acetazolamide / Q000494:pharmacology; D000818:Animals; D001639:Bicarbonates / Q000032:analysis; D002245:Carbon Dioxide / Q000032:analysis; D002257:Carbonic Anhydrase Inhibitors / Q000494:pharmacology*; D002256:Carbonic Anhydrases / Q000378:metabolism*; D002384:Catalysis; D002417:Cattle; D006863:Hydrogen-Ion Concentration; D007527:Isoenzymes / Q000037:antagonists & inhibitors / Q000378:metabolism*; D007668:Kidney / Q000201:enzymology; D013449:Sulfonamides / Q000494:pharmacology; D013876:Thiophenes / Q000494:pharmacology
chemical_list: D001639:Bicarbonates; D002257:Carbonic Anhydrase Inhibitors; D007527:Isoenzymes; D013449:Sulfonamides; D013876:Thiophenes; D002245:Carbon Dioxide; C062765:dorzolamide; D002256:Carbonic Anhydrases; D000086:Acetazolamide