Comparative analysis of conjugated bile acids in human serum using high-performance liquid chromatography and capillary electrophoresis.
This paper describes the analysis of conjugated bile acids in human serum using reversed-phase high-performance liquid chromatography (HPLC) and micellar electrokinetic capillary electrophoresis (CE). Samples of healthy subjects and patients with different hepatic diseases were pretreated with a simple preparation procedure using a solid-phase extraction technique. The optimal analytical conditions of both chromatographic methods were investigated for the convenience and reliability for routine analysis. Both HPLC and CE methods were found to be reliable and compatible. The recoveries of nine bile acid conjugates using both methods were generally >85% and reproducibility >90%. The day-to-day variation of retention time was <5% for HPLC, while the variation of migration time for CE was <3%. Although the detection limit of the HPLC method (1 nmol/ml) was five times more sensitive than that of the CE method, the CE method was considered to be more time and cost effective.
Lee
B L
BL
Department of Community, Occupational and Family Medicine, National University of Singapore, Singapore.
New
A L
AL
Ong
C N
CN
D001647:Bile Acids and Salts / Q000097:blood*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D019075:Electrophoresis, Capillary / Q000379:methods*; D005999:Glycochenodeoxycholic Acid / Q000097:blood; D006000:Glycocholic Acid / Q000097:blood; D006801:Humans; D006863:Hydrogen-Ion Concentration; D008107:Liver Diseases / Q000097:blood*; D008823:Micelles; D011786:Quality Control; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013655:Taurochenodeoxycholic Acid / Q000097:blood; D013656:Taurocholic Acid / Q000097:blood
D001647:Bile Acids and Salts; D008823:Micelles; D013655:Taurochenodeoxycholic Acid; D013656:Taurocholic Acid; D005999:Glycochenodeoxycholic Acid; D006000:Glycocholic Acid