title: A novel membrane protein is a mouse mammary tumor virus receptor.
abstract: Mouse mammary tumor virus (MMTV) infects a number of different cell types, including mammary gland and lymphoid cells, in vivo. To identify the cellular receptor for this virus, a mouse cDNA expression library was transfected into Cos-7 monkey kidney cells, and those transfected cells able to bind virus were selected by using antibody against the virus's cell surface envelope protein, gp52. One clone isolated from a library prepared from newborn thymus RNA, called MTVR, was able to confer virus binding to both monkey and human cells; this binding was blocked by anti-MTVR antibody. Moreover, transfection of MTVR into CV1 cells rendered them susceptible to infection by a murine leukemia virus-based retrovirus vector pseudotyped with the MMTV envelope protein. An epitope-tagged MTVR cofractionated with cellular membranes. Coimmunoprecipitation of the MMTV envelope protein and a MTVR-rabbit Fc fusion protein showed that these two proteins bound to each other. The MTVR sequence clone is unique, shows no homology to known membrane proteins, and is transcribed in many tissues.
authors.affiliation: Department of Microbiology/Cancer Center, University of Pennsylvania, Philadelphia 19104-6142, USA.
authors.forename: T V
authors.initials: TV
authors.lastname: Golovkina
authors.forename: J
authors.initials: J
authors.lastname: Dzuris
authors.forename: B
authors.initials: B
authors.lastname: van den Hoogen
authors.forename: A B
authors.initials: AB
authors.lastname: Jaffe
authors.forename: P C
authors.initials: PC
authors.lastname: Wright
authors.forename: S M
authors.initials: SM
authors.lastname: Cofer
authors.forename: S R
authors.initials: SR
authors.lastname: Ross
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D000957:Antigens, Viral, Tumor / Q000378:metabolism; D001483:Base Sequence; D019556:COS Cells; D002460:Cell Line; D002522:Chlorocebus aethiops; D002874:Chromosome Mapping; D003001:Cloning, Molecular; D018076:DNA, Complementary; D005260:Female; D006801:Humans; D008297:Male; D008324:Mammary Tumor Virus, Mouse / Q000378:metabolism*; D008565:Membrane Proteins / Q000235:genetics* / Q000378:metabolism; D051379:Mice; D008810:Mice, Inbred C57BL; D008969:Molecular Sequence Data; D011991:Receptors, Virus / Q000235:genetics* / Q000378:metabolism; D014158:Transcription, Genetic; D014407:Tumor Cells, Cultured; D014759:Viral Envelope Proteins / Q000378:metabolism
chemical_list: D000957:Antigens, Viral, Tumor; D018076:DNA, Complementary; C111417:Fam89b protein, mouse; D008565:Membrane Proteins; D011991:Receptors, Virus; D014759:Viral Envelope Proteins; C040612:glycoprotein 52 antigen, Mouse mammary tumor virus