Expression of the CB1 cannabinoid receptor in macrophage-like cells from brain tissue: immunochemical characterization by fusion protein antibodies.
Antibodies designed to recognize a 74 amino acid sequence of the N- or C-terminal domain of the rat CB1 cannabinoid receptor detected a 58 kDa protein in immunoblots of brain and various cells known to express the CB1 cannabinoid receptor. A human B-lymphoblastoid cell line and macrophage-like cells from neonatal rat brain were also positive for CB1 receptor-like immunoreactivity. Immunocytochemical analysis performed with isolated Fab fragments showed surface staining in NG108-15 cells and brain macrophage like cells which also express MHC class II antigens. The data suggest a plausible role for CB1 receptors in the immune function of brain.
Sinha
Debasish
D
Department of Psychiatry and Behavioral Sciences, Medical University of South Carolina, 171 Ashley Avenue, Charleston, SC 29425, USA.
Bonner
Tom I
TI
Section on Genetics, NIMH, National Institutes Of Health, Bethesda, MD 20892, USA.
Bhat
Narayan R
NR
Department of Neurology, Medical University of South Carolina, 171 Ashley Avenue, Charleston, SC 29425, USA.
Matsuda
Lisa A
LA
Department of Psychiatry and Behavioral Sciences, Medical University of South Carolina, 171 Ashley Avenue, Charleston, SC 29425, USA.
D000818:Animals; D000831:Animals, Newborn; D000918:Antibody Specificity; D001921:Brain / Q000166:cytology / Q000276:immunology*; D001923:Brain Chemistry / Q000276:immunology; D016466:CHO Cells; D002186:Cannabinoids / Q000235:genetics / Q000276:immunology; D006224:Cricetinae; D005260:Female; D019204:GTP-Binding Proteins / Q000276:immunology; D015870:Gene Expression; D008264:Macrophages / Q000737:chemistry / Q000276:immunology; D017628:Microglia / Q000737:chemistry* / Q000276:immunology; D011817:Rabbits; D051381:Rats; D043882:Receptors, Cannabinoid; D011955:Receptors, Drug / Q000032:analysis / Q000235:genetics* / Q000276:immunology*; D011993:Recombinant Fusion Proteins / Q000276:immunology; D012549:Schistosoma japonicum
D002186:Cannabinoids; D043882:Receptors, Cannabinoid; D011955:Receptors, Drug; D011993:Recombinant Fusion Proteins; D019204:GTP-Binding Proteins