title: Development and applications of enhanced green fluorescent protein mutants.
abstract: The introduction of several mutations resulted in the generation of improved mutants of the green fluorescent protein (GFP). A strong green (GFPsg25) and blue (BFPsg50) fluorescent protein, gave 50-fold-100-fold brighter fluorescence compared to wild-type GFP and BFP (Tyr66His), respectively, upon expression in mammalian cells. GFPsg25 and BFPsg50 have different excitation and emission maxima. This allows their use as an efficient dual-color tagging system and their independent detection in living cells.
authors.affiliation: National Cancer Institute-FCRDC, Frederick, MD, USA.
authors.forename: R H
authors.initials: RH
authors.lastname: Stauber
authors.forename: K
authors.initials: K
authors.lastname: Horie
authors.forename: P
authors.initials: P
authors.lastname: Carney
authors.forename: E A
authors.initials: EA
authors.lastname: Hudson
authors.forename: N I
authors.initials: NI
authors.lastname: Tarasova
authors.forename: G A
authors.initials: GA
authors.lastname: Gaitanaris
authors.forename: G N
authors.initials: GN
authors.lastname: Pavlakis
mesh_terms: D016475:3T3 Cells; D019943:Amino Acid Substitution / Q000235:genetics; D000818:Animals; D015415:Biomarkers; D005434:Flow Cytometry / Q000295:instrumentation / Q000379:methods; D005786:Gene Expression Regulation; D017930:Genes, Reporter; D049452:Green Fluorescent Proteins; D008164:Luminescent Proteins / Q000096:biosynthesis / Q000235:genetics*; D051379:Mice; D009154:Mutation / Q000235:genetics*; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis / Q000235:genetics; D013050:Spectrometry, Fluorescence; D014162:Transfection
chemical_list: D015415:Biomarkers; D008164:Luminescent Proteins; D011993:Recombinant Fusion Proteins; D049452:Green Fluorescent Proteins