title: Soluble Fas (Apo-1) levels in cerebrospinal fluid of multiple sclerosis patients.
abstract: CSF and serum levels of soluble Fas were studied in MS patients, in patients with various neurological diseases and in healthy controls. We did not detect differences in serum sFas levels between MS patients and controls. In CSF, despite sFas levels being similar in all patients studied, a statistically significant correlation between sFas CSF/sFas serum ratio and BBB damage (expressed as albumin CSF/albumin serum ratio) was detected in non-MS neurological disease, but not in MS patients. The normalized ratio (sFas CSF/sFas serum)/(Alb CSF/Alb serum) was significantly increased in MS patients compared to patients with non-inflammatory neurological disease suggesting an intrathecal synthesis of soluble Fas in MS. The percentage of apoptotic mononuclear cells was higher in CSF as compared to peripheral blood; moreover a lower proportion of apoptotic cells was found in CSF of MS patients. The findings lend support to the involvement of sFas in MS pathogenesis and suggest that a lower apoptosis in CSF may be a feature of the disease.
authors.affiliation: Istituto Nazionale Neurologico `C. Besta', Via Celoria 11, 20133 Milan, Italy.
authors.forename: Emilio
authors.initials: E
authors.lastname: Ciusani
authors.affiliation: Istituto Nazionale Neurologico `C. Besta', Via Celoria 11, 20133 Milan, Italy.
authors.forename: Simona
authors.initials: S
authors.lastname: Frigerio
authors.affiliation: Istituto Nazionale Neurologico `C. Besta', Via Celoria 11, 20133 Milan, Italy.
authors.forename: Maurizio
authors.initials: M
authors.lastname: Gelati
authors.affiliation: Istituto Nazionale Neurologico `C. Besta', Via Celoria 11, 20133 Milan, Italy.
authors.forename: Elena
authors.initials: E
authors.lastname: Corsini
authors.affiliation: Istituto Nazionale Neurologico `C. Besta', Via Celoria 11, 20133 Milan, Italy.
authors.forename: Anna
authors.initials: A
authors.lastname: Dufour
authors.affiliation: Istituto Nazionale Neurologico `C. Besta', Via Celoria 11, 20133 Milan, Italy.
authors.forename: Angelo
authors.initials: A
authors.lastname: Nespolo
authors.affiliation: Istituto Nazionale Neurologico `C. Besta', Via Celoria 11, 20133 Milan, Italy.
authors.forename: Loredana
authors.initials: L
authors.lastname: La Mantia
authors.affiliation: Istituto Nazionale Neurologico `C. Besta', Via Celoria 11, 20133 Milan, Italy.
authors.forename: Clara
authors.initials: C
authors.lastname: Milanese
authors.affiliation: Istituto Nazionale Neurologico `C. Besta', Via Celoria 11, 20133 Milan, Italy.
authors.forename: Giorgio
authors.initials: G
authors.lastname: Massa
authors.affiliation: Istituto Nazionale Neurologico `C. Besta', Via Celoria 11, 20133 Milan, Italy.
authors.forename: Andrea
authors.initials: A
authors.lastname: Salmaggi
mesh_terms: D000328:Adult; D000418:Albumins / Q000134:cerebrospinal fluid; D017209:Apoptosis; D001812:Blood-Brain Barrier; D002555:Cerebrospinal Fluid / Q000166:cytology; D005260:Female; D005434:Flow Cytometry; D006801:Humans; D007963:Leukocytes, Mononuclear / Q000166:cytology; D008297:Male; D008875:Middle Aged; D009103:Multiple Sclerosis / Q000097:blood / Q000134:cerebrospinal fluid*; D012709:Serum Albumin; D012995:Solubility; D019014:fas Receptor / Q000097:blood / Q000134:cerebrospinal fluid*
chemical_list: D000418:Albumins; D012709:Serum Albumin; D019014:fas Receptor