[The characteristics of protein p66--a substrate of the epidermal growth factor receptor].
The p66-kDa protein--a substrate of EGF receptor, is characterized. It is shown that the molecule of p66 is recognized by antibodies to phospholipase C gamma 1, includes conservative SH2--SH3 domains and lacks N-terminal part of PLC gamma 1. Protein p66 is associated to the EGF-R molecule on 992 tyrosine. Protein p66 binds to both membrane and internalized EGF-Rs and is redistributed with the receptors during receptor-mediated endocytosis. It is supposed that p66 may play a regulatory role in signal transduction.
Tvorogov
D B
DB
Institute of Cytology, Russian Academy of Sciences, St. Petersburg.
Blagoveshchenskaia
A D
AD
Tsupkina
N V
NV
Medvedeva
N D
ND
D016475:3T3 Cells; D000818:Animals; D002294:Carcinoma, Squamous Cell; D002461:Cell Line, Transformed; D004705:Endocytosis / Q000502:physiology; D066246:ErbB Receptors / Q000378:metabolism*; D006801:Humans; D007527:Isoenzymes / Q000378:metabolism*; D051379:Mice; D008970:Molecular Weight; D051966:Phospholipase C gamma; D011485:Protein Binding; D013379:Substrate Specificity; D014407:Tumor Cells, Cultured; D010738:Type C Phospholipases / Q000378:metabolism*
D007527:Isoenzymes; D066246:ErbB Receptors; D010738:Type C Phospholipases; D051966:Phospholipase C gamma
Kharakteristika belka p66--substrata retseptora épidermal'nogo faktora rosta.