title: Fetal development of the enteric nervous system of transgenic mice that overexpress the Hoxa-4 gene.
abstract: Megacolon occurs in neonatal and adult transgenic mice that overexpress the Hoxa-4 gene. Abnormalities, which are restricted to the terminal colon of these mice, include a hypoganglionosis, abnormal enteric ganglia with a structure appropriate for extra-enteric peripheral nerve and not the enteric nervous system (ENS), and gaps in the longitudinal muscle occupied by ganglia. To investigate the developmental origin of these abnormalities, we analyzed the development of the pelvis and terminal colon in Hoxa-4 transgenic mice. Morphological abnormalities were detected as early as E13. These included an enlargement of the mucosa and the bowel wall, a thickening of the enteric mesenchyme, and the ectopic location of pelvic ganglion cells, which initially clustered on the dorsolateral wall of the hindgut. As the bowel enlarged, these ectopic cells become ventrolateral and, between days E17 and E18.5, appeared to become incorporated into the gut, leaving neuron-filled gaps in the longitudinal muscle layer. The ectopic ganglia retained extra-enteric characteristics, including the presence of capillaries, basal laminae, collagen fibers, and catecholaminergic neurons, even after their incorporation into the bowel. It is proposed that the abnormal and ectopic expression of the Hoxa-4 transgene in the colon causes signalling molecule(s) of the enteric mesenchyme to be overproduced and that the overabundance of these signals leads to mucosal enlargement and misdirection of migrating pelvic neuronal progenitors.
authors.affiliation: Department of Anatomy, Columbia University, College of Physicians and Surgeons, New York, New York 10032, USA.
authors.forename: V M
authors.initials: VM
authors.lastname: Tennyson
authors.forename: M D
authors.initials: MD
authors.lastname: Gershon
authors.forename: P R
authors.initials: PR
authors.lastname: Wade
authors.forename: D A
authors.initials: DA
authors.lastname: Crotty
authors.forename: D J
authors.initials: DJ
authors.lastname: Wolgemuth
mesh_terms: D000818:Animals; D003106:Colon / Q000002:abnormalities* / Q000196:embryology* / Q000294:innervation; D004268:DNA-Binding Proteins; D005314:Embryonic and Fetal Development; D017615:Enteric Nervous System / Q000002:abnormalities* / Q000196:embryology* / Q000648:ultrastructure; D005724:Ganglia / Q000166:cytology / Q000648:ultrastructure; D018398:Homeodomain Proteins / Q000235:genetics / Q000502:physiology*; D007413:Intestinal Mucosa / Q000196:embryology; D008531:Megacolon / Q000196:embryology; D008648:Mesoderm; D051379:Mice; D008822:Mice, Transgenic; D009474:Neurons / Q000648:ultrastructure; D010388:Pelvis / Q000196:embryology; D014157:Transcription Factors; D014446:Tyrosine 3-Monooxygenase / Q000032:analysis
chemical_list: D004268:DNA-Binding Proteins; D018398:Homeodomain Proteins; C484111:Hoxa4 protein, mouse; D014157:Transcription Factors; D014446:Tyrosine 3-Monooxygenase