title: Platelet cNOS activity is reduced in patients with IDDM and NIDDM.
abstract: Several studies in vitro and in vivo suggest that the nitric oxide (NO) production is impaired in diabetes mellitus. Reduced levels of NO could contribute to vascular alteration facilitating platelet-vascular wall interaction, adhesion of monocytes to endothelium, vascular smooth muscle proliferation and by decreasing endothelium-dependent vasodilation. In this study we evaluated the activity of the constitutive nitric oxide synthase (cNOS) in platelets of patients with insulin-dependent diabetes mellitus (IDDM) and with non-insulin-dependent diabetes mellitus (NIDDM). When compared to that of normal subjects, cNOS activity is significantly lower in patients with IDDM and with NIDDM (1.57 +/- 0.25 vs. 0.66 +/- 0.10 fmol/min/10(9) PLTs and 1.57 +/- 0.25 vs. 0.67 +/- 0.08, respectively; p<0.005). These data demonstrate that the platelet cNOS activity is decreased in diabetes mellitus.
authors.affiliation: Dipartimento di Medicina Interna, Università degli Studi di Torino, Italy.
authors.forename: V
authors.initials: V
authors.lastname: Martina
authors.forename: G A
authors.initials: GA
authors.lastname: Bruno
authors.forename: F
authors.initials: F
authors.lastname: Trucco
authors.forename: E
authors.initials: E
authors.lastname: Zumpano
authors.forename: M
authors.initials: M
authors.lastname: Tagliabue
authors.forename: C
authors.initials: C
authors.lastname: Di Bisceglie
authors.forename: G
authors.initials: G
authors.lastname: Pescarmona
mesh_terms: D000328:Adult; D001792:Blood Platelets / Q000201:enzymology*; D003922:Diabetes Mellitus, Type 1 / Q000097:blood*; D003924:Diabetes Mellitus, Type 2 / Q000097:blood*; D004789:Enzyme Activation; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D019001:Nitric Oxide Synthase / Q000378:metabolism*; D052250:Nitric Oxide Synthase Type III
chemical_list: C496314:NOS3 protein, human; D019001:Nitric Oxide Synthase; D052250:Nitric Oxide Synthase Type III