Enrichment medium for isolation of Campylobacter jejuni-Campylobacter coli.
A kaseini-broth enrichment medium (KBEM) for the isolation improvement of Campylobacter jejuni-Campylobacter coli from stool samples is described. Isolation of Campylobacters from stool specimens by direct inoculation onto solid selective medium was compared with isolation after twenty-four hours enrichment at 37 degrees C in KBEM, followed by subculture onto the same solid selective medium. Of 156 examined stool samples from diarrhoeal children positive results were obtained from 17 patients altogether: 11 by direct inoculation on selective media and 6 only owing to enrichment. Thus, an increase of 35.3% in the isolation rate was obtained by using the enrichment medium. The same medium allows the preservation of isolates of Campylobacter jejuni-Campylobacter coli for 2 months and longer at 37 degrees C.
Sicinschi
L A
LA
Laboratory of Microbiology, N. Testemitsanu University of Medicine, Republica Moldova.
D001431:Bacteriological Techniques; D017000:Campylobacter coli / Q000254:growth & development / Q000302:isolation & purification*; D016123:Campylobacter jejuni / Q000254:growth & development / Q000302:isolation & purification*; D002648:Child; D003470:Culture Media; D005243:Feces / Q000382:microbiology; D006801:Humans; D013997:Time Factors
D003470:Culture Media