Expression of lumican in human breast carcinoma.
Lumican mRNA has been identified as being differentially expressed between different regions of the same human breast tumor. In situ hybridization study of 26 independent breast tumors confirmed the presence of lumican mRNA in fibroblast-like cells within stroma and showed a significant increase of its expression in tumor compared to adjacent normal stroma (P < 0.001). Higher lumican expression was associated with higher tumor grade, lower estrogen receptor levels in the tumor, and younger age of the patients (P < 0.05). Reverse transcription-PCR analysis of total RNA extracted from 19 independent breast tissues exhibiting lesions that are thought to parallel tumor progression also suggests that this proteoglycan is differentially expressed during tumor progression.
Leygue
E
E
Department of Biochemistry and Molecular Biology, University of Manitoba, Faculty of Medicine, Winnipeg, Canada. eleygue@cc.umanitoba.ca
Snell
L
L
Dotzlaw
H
H
Hole
K
K
Hiller-Hitchcock
T
T
Roughley
P J
PJ
Watson
P H
PH
Murphy
L C
LC
D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D001940:Breast / Q000378:metabolism; D001943:Breast Neoplasms / Q000378:metabolism*; D011508:Chondroitin Sulfate Proteoglycans / Q000096:biosynthesis*; D004591:Electrophoresis, Polyacrylamide Gel; D005260:Female; D006801:Humans; D017403:In Situ Hybridization; D007632:Keratan Sulfate / Q000096:biosynthesis*; D000071234:Lumican; D008875:Middle Aged; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D012967:Sodium Dodecyl Sulfate; D014158:Transcription, Genetic; D014407:Tumor Cells, Cultured
D011508:Chondroitin Sulfate Proteoglycans; C000604306:LUM protein, human; D000071234:Lumican; D012333:RNA, Messenger; D012967:Sodium Dodecyl Sulfate; D007632:Keratan Sulfate