title: Molecular recognition of siderophores: a study with cloned ferrioxamine receptors (FoxA) from Erwinia herbicola and Yersinia enterocolitica.
abstract: The outer membrane receptor for ferrioxamines (FoxAErw) of Erwinia herbicola (Pantoea agglomerans) was cloned from a cosmid gene bank and partially sequenced. A comparison of the partial amino acid sequence of FoxAErw with the amino acid sequence of FoxAYer from Yersinia enterocolitica revealed a high sequence homology. A functional analysis of FoxAErw and FoxAYer receptors cloned into a Fhu-negative background (HK97) revealed that ferrioxamines are recognized at very low concentrations (< 10 pmoles) in growth promotion bioassays. A collection of ferrioxamine derivatives containing varying chain lengths and ether bridges within the molecule was also accepted. However, the three ether containing ferrioxamine (Et3) behaved differently in the two FoxA receptors. Coprogen was also recognized to a certain extent, whereas ferrichromes were completely excluded from the FoxA receptors, confirming that coprogens share some structural similarities with the ferrioxamines. FoxA mutants (FM13) of Erwinia herbicola obtained by ferrimycin selection showed no uptake of 55Fe-labelled ferrioxamine E and B any more, while the transport of coprogen and ferrichrome was unaffected or even slightly increased.
authors.affiliation: University of Tübingen, Germany.
authors.forename: K
authors.initials: K
authors.lastname: Deiss
authors.forename: K
authors.initials: K
authors.lastname: Hantke
authors.forename: G
authors.initials: G
authors.lastname: Winkelmann
mesh_terms: D000595:Amino Acid Sequence; D001425:Bacterial Outer Membrane Proteins / Q000235:genetics / Q000378:metabolism*; D001693:Biological Transport, Active; D003001:Cloning, Molecular; D003676:Deferoxamine / Q000378:metabolism*; D004755:Enterobacteriaceae / Q000235:genetics / Q000378:metabolism*; D004885:Erwinia / Q000235:genetics / Q000378:metabolism*; D004926:Escherichia coli / Q000235:genetics; D029968:Escherichia coli Proteins; D005290:Ferric Compounds / Q000378:metabolism*; D008969:Molecular Sequence Data; D009154:Mutation; D011956:Receptors, Cell Surface; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism; D017386:Sequence Homology, Amino Acid; D017262:Siderophores / Q000378:metabolism*; D013045:Species Specificity; D015008:Yersinia enterocolitica / Q000235:genetics / Q000378:metabolism*
chemical_list: D001425:Bacterial Outer Membrane Proteins; D029968:Escherichia coli Proteins; D005290:Ferric Compounds; C489524:FoxA protein, bacteria; C489523:FoxB protein, E coli; D011956:Receptors, Cell Surface; D011994:Recombinant Proteins; D017262:Siderophores; C002577:ferrioxamine B; D003676:Deferoxamine