title: Induction of CYP102 (cytochrome P450BM-3) in Bacillus megaterium by 17 beta-estradiol and 4-sec-butylphenol.
abstract: CYP102 (Cytochrome P450BM-3) is induced in Bacillus megaterium by barbiturates, peroxisome proliferators, and nonsteroidal anti-inflammatory drugs. We now describe the induction of CYP102 in B. megaterium by 17 beta-estradiol and by 4-sec-butylphenol. These estrogens interact with the repressor protein Bm3R1, causing it to dissociate with the operator of the CYP102 gene and allowing transcription to occur. We have developed a stable transfection of a construct into B. megaterium of a truncated CYP102 gene coupled with the luciferase gene in a promoterless plasmid and have used this construct to test the induction of CYP102 by these estrogens. Estradiol demonstrated a dose-dependent induction of CYP102 which saturated at a 2-fold increase at 150 microM 4 hr post-addition. 4-sec-Butylphenol produced a dose-dependent and time-dependent induction up to 300 microM and 6 hr post-induction.
authors.affiliation: Department of Pharmacology, Tulane University Medical School, New Orleans, Louisiana 70112, USA. nhopkin@mailhost.tcs.tulane.edu
authors.forename: N E
authors.initials: NE
authors.lastname: Hopkins
authors.forename: N
authors.initials: N
authors.lastname: English
authors.forename: V
authors.initials: V
authors.lastname: Hughes
authors.forename: C W
authors.initials: CW
authors.lastname: Rowley
authors.forename: C R
authors.initials: CR
authors.lastname: Wolf
authors.forename: W L
authors.initials: WL
authors.lastname: Alworth
mesh_terms: D001410:Bacillus megaterium / Q000187:drug effects; D001426:Bacterial Proteins / Q000378:metabolism; D003577:Cytochrome P-450 Enzyme System / Q000096:biosynthesis* / Q000235:genetics; D004305:Dose-Response Relationship, Drug; D004353:Drug Evaluation, Preclinical; D004790:Enzyme Induction; D004958:Estradiol / Q000494:pharmacology*; D004967:Estrogens / Q000494:pharmacology*; D017930:Genes, Reporter; D006899:Mixed Function Oxygenases / Q000096:biosynthesis* / Q000235:genetics; D009251:NADPH-Ferrihemoprotein Reductase; D010636:Phenols / Q000494:pharmacology*; D011485:Protein Binding; D011994:Recombinant Proteins / Q000096:biosynthesis; D012097:Repressor Proteins / Q000378:metabolism; D014157:Transcription Factors
chemical_list: D001426:Bacterial Proteins; D004967:Estrogens; D010636:Phenols; D011994:Recombinant Proteins; D012097:Repressor Proteins; D014157:Transcription Factors; C033932:2-sec-butylphenol; C073493:Bm3R1 protein, Bacillus megaterium; D004958:Estradiol; D003577:Cytochrome P-450 Enzyme System; D006899:Mixed Function Oxygenases; D009251:NADPH-Ferrihemoprotein Reductase; C052423:flavocytochrome P450 BM3 monoxygenases