Estimation of type-specific anti-streptococcal M-protein antibodies with biotinylated peptides in human sera.
The design of a novel enzyme-linked immunosorbent assay for the estimation of antibodies directed against the N-terminus of the M-protein of Streptococcus pyogenes is described. The ELISA employs biotinylated peptide antigens of the types 1, 4, 12 and 19 immobilized by (strept-)avidin on the surface of polystyrene microtiter wells. In rabbit hyperimmune sera and in human serum samples, antibodies against the corresponding serotype could be detected with high sensitivity and specificity.
Kühnemund
O
O
Institute of Medical Microbiology, Hospital of the Technical University, Aachen, Germany.
Schumacher
F
F
Casaretto
M
M
Holm
S E
SE
Lütticken
R
R
D000328:Adult; D000368:Aged; D000595:Amino Acid Sequence; D000818:Animals; D000907:Antibodies, Bacterial / Q000097:blood*; D000942:Antigens, Bacterial; D001360:Avidin / Q000378:metabolism; D001425:Bacterial Outer Membrane Proteins; D001426:Bacterial Proteins / Q000737:chemistry / Q000276:immunology*; D019921:Biotinylation; D002352:Carrier Proteins; D002648:Child; D004797:Enzyme-Linked Immunosorbent Assay; D004800:Enzymes, Immobilized; D006801:Humans; D007118:Immunoassay; D008875:Middle Aged; D008969:Molecular Sequence Data; D010446:Peptide Fragments / Q000138:chemical synthesis / Q000276:immunology* / Q000378:metabolism; D011817:Rabbits; D012703:Serotyping; D019809:Streptavidin / Q000378:metabolism; D013290:Streptococcal Infections / Q000276:immunology*; D013297:Streptococcus pyogenes / Q000145:classification / Q000276:immunology*
D000907:Antibodies, Bacterial; D000942:Antigens, Bacterial; D001425:Bacterial Outer Membrane Proteins; D001426:Bacterial Proteins; D002352:Carrier Proteins; D004800:Enzymes, Immobilized; D010446:Peptide Fragments; C014689:streptococcal M protein; D001360:Avidin; D019809:Streptavidin