title: Induction of flat morphology in K-ras-transformed fibroblasts by lycorine, an alkaloid isolated from the tropical plant Eucharis grandiflora.
abstract: In the course of screening for Ras function inhibitors from plant extracts, we isolated lycorine from a chloroform extract of Eucharis grandiflora leaves. Lycorine induced flat morphology in K-ras-NRK cells after treatment for 2-3 days, whereas its morphological effect on NRK cells was weaker. Lycorine was found to inhibit protein synthesis specifically in cultured K-ras-NRK cells. It also lowered the cellular amount of Ras in 2-3 days.
authors.affiliation: Department of Applied Chemistry, Faculty of Science and Technology, Keio University, Yokohama, Japan.
authors.forename: N
authors.initials: N
authors.lastname: Kushida
authors.forename: S
authors.initials: S
authors.lastname: Atsumi
authors.forename: T
authors.initials: T
authors.lastname: Koyano
authors.forename: K
authors.initials: K
authors.lastname: Umezawa
mesh_terms: D000470:Alkaloids / Q000302:isolation & purification / Q000494:pharmacology*; D047151:Amaryllidaceae Alkaloids; D000818:Animals; D002455:Cell Division / Q000187:drug effects; D002471:Cell Transformation, Neoplastic / Q000187:drug effects*; D005347:Fibroblasts / Q000166:cytology / Q000187:drug effects*; D011905:Genes, ras / Q000187:drug effects* / Q000502:physiology*; D010617:Phenanthridines / Q000302:isolation & purification / Q000494:pharmacology*; D010936:Plant Extracts / Q000302:isolation & purification / Q000494:pharmacology*; D018515:Plant Leaves / Q000737:chemistry; D010946:Plants, Medicinal / Q000737:chemistry; D051381:Rats
chemical_list: D000470:Alkaloids; D047151:Amaryllidaceae Alkaloids; D010617:Phenanthridines; D010936:Plant Extracts; C015330:lycorine