Characterization of Cyprinus carpio brain nitric oxide synthase.
Nitric oxide synthase (NOS) activity is found both in soluble and in particulate fractions of the carp brain. The Km values for arginine are 2.8+/-0.5 and 3.3+/-0.4 microM for the soluble and particulate fractions, respectively. K for NG-monomethyl-L-arginine inhibitor are 2.6+/-0.5 and 2.9+/-0.6 microM, and activation energy for the breakdown of the substrate-enzyme complex 8120+/-710 and 4620+/-450 cal per mole. Carp enzyme shows higher affinity than rat NOS for Ca2+ and for the competitive inhibitor 7-nitroindazole.
Conte
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Department of Animal Biology, University of Modena, Italy.
Ottaviani
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D000818:Animals; D001120:Arginine / Q000032:analysis; D001921:Brain / Q000201:enzymology*; D001923:Brain Chemistry / Q000502:physiology; D002347:Carps / Q000378:metabolism*; D002956:Citrulline / Q000096:biosynthesis; D004791:Enzyme Inhibitors / Q000494:pharmacology; D005609:Free Radicals / Q000378:metabolism; D007191:Indazoles / Q000494:pharmacology; D008297:Male; D019001:Nitric Oxide Synthase / Q000037:antagonists & inhibitors / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar; D013045:Species Specificity; D014316:Tritium
D004791:Enzyme Inhibitors; D005609:Free Radicals; D007191:Indazoles; D014316:Tritium; D002956:Citrulline; D001120:Arginine; D019001:Nitric Oxide Synthase; C080122:7-nitroindazole