The LEC rat possesses reduced hepatic selenium, contributing to the severity of spontaneous hepatitis and sensitivity to carcinogenesis.
The hepatic concentrations of copper, zinc, magnesium, calcium, and selenium were measured in LEC rats, which develop a spontaneous form of hepatitis at 3-4 months of age, and compared to trace metal concentrations in the LEA rat, its asymptomatic congenic strain. Consistent with results found by other groups, copper was found to accumulate within the liver of LEC rats to levels more than 50 times those measured in LEA rats. In addition, liver selenium concentration in LEC rats was found to be around 50% of that in LEA rats. The enzyme activity, and RNA for the selenium dependent enzyme, glutathione peroxidase, was also found to be reduced in LEC rat liver. These results indicate that hepatic selenium in the LEC rat is depleted and that, as a result of this, the capacity to protect cells from copper-induced free-radical damage is reduced.
Downey
J S
JS
Department of Toxicology, St. Bartholomew's & the Royal London School of Medicine and Dentistry, United Kingdom.
Bingle
C D
CD
Cottrell
S
S
Ward
N
N
Churchman
D
D
Dobrota
M
M
Powell
C J
CJ
D000818:Animals; D002118:Calcium / Q000378:metabolism; D003300:Copper / Q000378:metabolism; D005609:Free Radicals / Q000378:metabolism; D005979:Glutathione Peroxidase / Q000172:deficiency / Q000235:genetics; D006520:Hepatitis, Animal / Q000209:etiology* / Q000235:genetics / Q000378:metabolism; D008099:Liver / Q000378:metabolism*; D008114:Liver Neoplasms, Experimental / Q000209:etiology* / Q000235:genetics / Q000378:metabolism; D008274:Magnesium / Q000378:metabolism; D008297:Male; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D051381:Rats; D011919:Rats, Inbred Strains; D012643:Selenium / Q000172:deficiency*; D015032:Zinc / Q000378:metabolism
D005609:Free Radicals; D012333:RNA, Messenger; D003300:Copper; D005979:Glutathione Peroxidase; D012643:Selenium; D008274:Magnesium; D015032:Zinc; D002118:Calcium