Determination of aflatoxicol in human urine by immunoaffinity column clean-up and liquid chromatography.
A method for the determination of aflatoxicol in urine has been developed. The urine samples were cleaned up by an automated procedure using immunoaffinity columns before analysis by high-performance liquid chromatography and fluorescence detection. Post-column derivatization with bromine allowed the simultaneous determination of aflatoxicol and aflatoxins B1, B2, G1, G2, M1, and Q1. Average recovery of aflatoxicol was 99% in the range 4-40 pg ml-1 of spiked urine samples. The relative standard deviations were all between 1% and 3%. The limit of detection was 1 pg ml-1 urine. Authentic samples from exposed feed-factory workers were analysed, but aflatoxin levels were found to be below the detection limit.
Kussak
A
A
National Institute for Working Life, Umeå, Sweden.
Andersson
B
B
Andersson
K
K
Nilsson
C A
CA
D000348:Aflatoxins / Q000652:urine*; D002846:Chromatography, Affinity / Q000379:methods*; D002851:Chromatography, High Pressure Liquid; D019649:Food Industry; D006801:Humans; D016273:Occupational Exposure; D013050:Spectrometry, Fluorescence
D000348:Aflatoxins; C012170:aflatoxicol