New shuttle vectors for the introduction of cloned DNA in Desulfovibrio.
The pBG1 replicon from the cryptic plasmid of Desulfovibrio desulfuricans G100A was inserted into pTZ18U derivatives to generate a new family of shuttle vectors. These plasmids are stable both in Escherichia coli and in Desulfovibrio, they present a large number of unique restriction sites, and colonies of recombinant clones can be identified by blue/white screening in E. coli. The pBMC, pBMK, and pBMS series carry the cat, npt, or strAB genes as selectable markers, respectively. The pBMC6, pBMK6, and pBMS6 plasmids can be introduced both in D. desulfuricans and in Desulfovibrio fructosovorans by electrotransformation, and the pBMC7, pBMK7, and pBMS7 plasmids contain additional mobilization functions which makes them suitable for conjugation.
Rousset
M
M
Unité de Bioénergétique et Ingénierie des Proteines, CNRS, Marseille. rousset@ibsm.cnrs-mrs.fr
Casalot
L
L
Rapp-Giles
B J
BJ
Dermoun
Z
Z
de Philip
P
P
Bélaich
J P
JP
Wall
J D
JD
D003001:Cloning, Molecular / Q000379:methods*; D003227:Conjugation, Genetic; D003901:Desulfovibrio / Q000235:genetics*; D018274:Electroporation; D004926:Escherichia coli / Q000235:genetics; D005822:Genetic Vectors / Q000235:genetics*; D006864:Hydrogenase / Q000235:genetics; D010957:Plasmids / Q000235:genetics; D012093:Replicon / Q000235:genetics; D014169:Transformation, Bacterial
C043296:nickel-iron hydrogenase; D006864:Hydrogenase