[The possibility of performing artificial selection in vivo for a change in the mutability level in populations of mouse bone marrow stem cells].
Bone marrow cells of CBA males were transplanted to irradiated syngeneic females and after 13 days the mean frequency of blood erythrocytes with micronuclei, cloning efficiency of haemopoietic stem cells (HSC), and variability range of frequency of cells with micronuclei in spleen colonies were studied in 44 primary recipients. A wide range of variability has been shown for the indicators of mutability and cloning efficiency (0.1-1.8% for frequency of erythrocytes with micronuclei, 200-300-1500 for cloning efficiency, 0.1-0.5% for frequency of cells with micronuclei in spleen colonies of individual transplants). A weak negative correlation between the frequency of erythrocytes with micronuclei and the cloning efficiency was observed. We conclude that the difference in frequency of erythrocytes with micronuclei between HSC subpopulations is sufficiently high to carry out an artificial selection for high and low mutability in the course of transplantations. The cloning efficiency can be increased when spleen colonies are studied by micronuclear analysis. Data on the importance of such a selection in studying the behavior of "mutability" and "cloning efficiency" characters, immortalization, "aging", and death of HSC populations of CBA mice have been reported.
Vakhtin
Iu B
IuB
Institute of Cytology, Russian Academy of Sciences, St. Petersburg.
Kaminskaia
E V
EV
Konstantinova
I V
IV
Pereverzev
A E
AE
Fedorova
E V
EV
D000818:Animals; D003114:Colony-Forming Units Assay; D004906:Erythrocyte Count; D004912:Erythrocytes / Q000648:ultrastructure; D005260:Female; D018380:Hematopoietic Stem Cell Transplantation / Q000379:methods; D006412:Hematopoietic Stem Cells / Q000166:cytology*; D008297:Male; D051379:Mice; D008808:Mice, Inbred CBA; D048629:Micronuclei, Chromosome-Defective / Q000648:ultrastructure; D016296:Mutagenesis; D012641:Selection, Genetic; D013154:Spleen; D013997:Time Factors; D016042:Transplantation, Heterotopic
O vozmozhnosti provedeniia iskusstvennogo otbora in vivo na izmenenie urovnia mutabil'nosti v populiatsiiakh stvolovykh kostnomozgovykh kletok mysheĭ.