title: Identification of alternatively spliced Na(+)-Ca2+ exchanger isoforms expressed in the heart.
abstract: Alternatively spliced isoforms of Na(+)-Ca2+ exchanger were found from various tissues and species. RT-PCR amplification was performed on the basis of our cloned mouse cardiac Na(+)-Ca2+ exchanger and four alternatively spliced isoforms of Na(+)-Ca2+ exchanger were identified. Three (NCX1.3, NCX1.4, and NCX1.12) of them were first identified in the heart, and one isoform (NCX1.12) was a novel spliced variant. These four spliced variants were present in the embryonic and adult atria and ventricles. Different cell types of the heart expressed different spliced isoforms of Na(+)-Ca2+ exchanger. Southern blot analysis indicated that the Na(+)-Ca2+ exchanger gene existed as a single copy in the mouse genome. Thus, the Na(+)-Ca2+ exchanger isoforms expressed in mouse heart are consistent with being produced by alternative splicing and they may have different functions in various cell types in the mouse heart.
authors.affiliation: Department of Life Science, Pohang University of Science and Technology, Korea.
authors.forename: I
authors.initials: I
authors.lastname: Kim
authors.forename: G Y
authors.initials: GY
authors.lastname: Koh
authors.forename: C O
authors.initials: CO
authors.lastname: Lee
mesh_terms: D017398:Alternative Splicing; D000595:Amino Acid Sequence; D000818:Animals; D017931:DNA Primers / Q000235:genetics; D005260:Female; D005318:Fetal Heart / Q000166:cytology / Q000378:metabolism; D051379:Mice; D008810:Mice, Inbred C57BL; D008969:Molecular Sequence Data; D009206:Myocardium / Q000166:cytology / Q000378:metabolism*; D016133:Polymerase Chain Reaction; D011247:Pregnancy; D012313:RNA / Q000235:genetics / Q000378:metabolism; D019831:Sodium-Calcium Exchanger / Q000235:genetics* / Q000378:metabolism*; D014018:Tissue Distribution
chemical_list: D017931:DNA Primers; D019831:Sodium-Calcium Exchanger; D012313:RNA