title: Downregulated expression of the signaling molecules Nck, c-Crk, Grb2/Ash, PI 3-kinase p110 alpha and WRN during fibroblast aging in vitro.
abstract: An RT-PCR analysis was performed to examine changes in intracellular signal transducing molecules during in-vitro aging of human fibroblasts. Expression of Nck, c-Crk, Grb2/Ash, phosphoinositide (PI) 3-kinase p110 alpha and Werner's syndrome gene product WRN was noticeably reduced in late passage cells, showing a concurrent downregulation of a set of signaling molecules accompanying aging.
authors.affiliation: Department of Biosignal Research, Tokyo Metropolitan Institute of Gerontology, Japan. kmatuoka@mailhost.net
authors.forename: K
authors.initials: K
authors.lastname: Matuoka
authors.forename: T
authors.initials: T
authors.lastname: Takenawa
mesh_terms: D048868:Adaptor Proteins, Signal Transducing; D015152:Blotting, Northern; D002478:Cells, Cultured; D016922:Cellular Senescence / Q000502:physiology; D004265:DNA Helicases / Q000235:genetics / Q000502:physiology*; D015536:Down-Regulation / Q000502:physiology; D005090:Exodeoxyribonucleases; D005347:Fibroblasts / Q000737:chemistry / Q000166:cytology / Q000378:metabolism; D051380:GRB2 Adaptor Protein; D005786:Gene Expression Regulation / Q000235:genetics / Q000502:physiology; D006801:Humans; D015513:Oncogene Proteins / Q000235:genetics / Q000502:physiology; D019869:Phosphatidylinositol 3-Kinases / Q000235:genetics / Q000502:physiology*; D016133:Polymerase Chain Reaction; D011506:Proteins / Q000235:genetics / Q000502:physiology*; D011518:Proto-Oncogene Proteins / Q000235:genetics / Q000502:physiology; D050737:Proto-Oncogene Proteins c-crk; D012333:RNA, Messenger / Q000032:analysis / Q000235:genetics; D053484:RecQ Helicases; D015398:Signal Transduction / Q000235:genetics / Q000502:physiology; D000071657:Werner Syndrome Helicase
chemical_list: D048868:Adaptor Proteins, Signal Transducing; D051380:GRB2 Adaptor Protein; C495948:GRB2 protein, human; C078332:Nck protein; D015513:Oncogene Proteins; D011506:Proteins; D011518:Proto-Oncogene Proteins; D050737:Proto-Oncogene Proteins c-crk; D012333:RNA, Messenger; D019869:Phosphatidylinositol 3-Kinases; D005090:Exodeoxyribonucleases; D004265:DNA Helicases; D053484:RecQ Helicases; C098785:WRN protein, human; D000071657:Werner Syndrome Helicase