title: Recommended storage and resuscitation conditions for the MDCC-MSB1 cell line.
abstract: Storage and resuscitation of MDCC-MSB1 cells, most commonly used for the propagation of chicken anemia virus was studied. Cells were frozen slowly in RPMI medium 1640 with 10% or 20% fetal bovine serum and 20% dimethyl sulfoxide, held at -70 C overnight, and placed into liquid nitrogen the next day. After 1 mo, 91% to 100% resuscitation rates were obtained, while cells that were frozen fast (directly transferred into liquid nitrogen) could not be resuscitated.
authors.affiliation: Biomedical Research Group, School of Biomedical Sciences, Charles Sturt University, Wagga Wagga, New South Wales, Australia.
authors.forename: A L
authors.initials: AL
authors.lastname: Coombes
authors.forename: G R
authors.initials: GR
authors.lastname: Crawford
mesh_terms: D000818:Animals; D001769:Blood; D002417:Cattle; D018929:Cell Culture Techniques / Q000379:methods*; D002460:Cell Line; D002470:Cell Survival; D017727:Chicken anemia virus / Q000254:growth & development*; D002645:Chickens; D015925:Cryopreservation / Q000379:methods*; D003470:Culture Media
chemical_list: D003470:Culture Media