title: Methods for quantifying ultrasound backscatter and two-dimensional video intensity: implications for contrast-enhanced sonography.
abstract: Quantification of acoustic backscatter energy is believed to be useful for assessing "tissue character" and for quantifying the regional concentration of echo contrast. Measurement of ultrasonic video intensity has been the traditional means of quantifying backscatter energy, with "integrated backscatter" considered the gold standard. The purpose of this work is to review the commonly used methods for quantifying ultrasonic backscatter and to describe the difference between detected backscatter energy and the intrinsic tissue backscatter coefficient. Many of the quantification pitfalls that can lead to erroneous conclusions will also be discussed. A set of eight rubber phantoms with backscatter coefficient from -6 dB to +15 dB relative to liver were imaged at 2.5, 3.5, and 5.0 MHz. Methods for calculating the acoustic backscatter intensity from calibrated video intensity measurements and for calculating the tissue backscatter coefficient are described and tested using equipment from two different manufacturers. A commercially available automatic "acoustic densitometry" system with on-board quantitative integrated backscatter is also evaluated. Ultrasound attenuation and ultrasound system factors were found to strongly influence the detected backscatter intensity using either calibrated video intensity or on-board integrated backscatter. Special system transfer functions and attenuation correction were found to be useful in converting video intensity and integrated backscatter to a measure of the intrinsic tissue backscatter coefficient. With these correction factors, the correlation between the measured tissue backscatter coefficient and the phantom backscatter coefficient was excellent (r = 0.99, intercept 0.0, regression slope essentially 1.0) at all three imaging frequencies with traditional video intensity or on-board integrated backscatter. Uncalibrated video intensity and on-board integrated backscatter have limitations when used in isolation for tissue characterization. Rigorous attention to the imaging parameters and the use of calibration functions are necessary before video intensity measurement or integrated backscatter can be used reliably to measure the tissue backscatter coefficient.
authors.affiliation: University of Rochester Medical Center, New York 14642, USA.
authors.forename: K Q
authors.initials: KQ
authors.lastname: Schwarz
authors.forename: X
authors.initials: X
authors.lastname: Chen
authors.forename: S
authors.initials: S
authors.lastname: Steinmetz
mesh_terms: D000162:Acoustics; D019047:Phantoms, Imaging; D014463:Ultrasonography