Structural and immunochemical studies of two cross-reactive Proteus mirabilis O-antigens, O6 and O23, containing beta1-->3-linked 2-acetamido-2-deoxy-D-glucopyranose residues.
A marked serological cross-reactivity was observed by ELISA and a precipitation test between anti-Proteus mirabilis O23 serum and the lipopolysaccharide as well as the O-specific polysaccharide from the Proteus mirabilis strain belonging to serogroup O6. The structures of the O-specific polysaccharides were elucidated using chemical and NMR spectroscopic analyses, and the only common component, 2-acetamido-2-deoxy-beta-D-glucopyranose (beta-D-GlcNAc), was revealed, which was suggested to be responsible for the cross-reactivity observed. Both anti-O23 and anti-O6 sera were shown to react with 1, 3-Linked beta-D-GlcNAc-containing O-antigen from Salmonella enterica ssp. arizonae O59 also. The lack of reactivity of Smith-degraded P. mirabilis O6 O-specific polysaccharide with homologous antiserum indicated the crucial role of alpha-D-glucuronic acid in specific antibody binding.
Cedzyński
M
M
Microbiology and Virology Centre, Polish Academy of Sciences, Lódź.
Swierzko
A S
AS
Ziółkowski
A
A
Rózalski
A
A
Paramonov
N A
NA
Vinogradov
E V
EV
Knirel
Y A
YA
Kaca
W
W
D000117:Acetylglucosamine / Q000737:chemistry*; D000818:Animals; D000907:Antibodies, Bacterial / Q000276:immunology; D002236:Carbohydrate Conformation; D002240:Carbohydrate Sequence; D003429:Cross Reactions; D004797:Enzyme-Linked Immunosorbent Assay; D009682:Magnetic Resonance Spectroscopy; D008969:Molecular Sequence Data; D019081:O Antigens / Q000737:chemistry* / Q000276:immunology*; D011513:Proteus mirabilis / Q000276:immunology*; D011817:Rabbits
D000907:Antibodies, Bacterial; D019081:O Antigens; D000117:Acetylglucosamine