title: Detection of mRNAs and alternatively spliced transcripts of dopamine receptors in rat peripheral sensory and sympathetic ganglia.
abstract: The presence of mRNAs for dopamine receptor subtypes and dopamine transporter in rat peripheral sensory and sympathetic ganglia was investigated using polymerase chain reaction (PCR) and DNA sequencing. Dopamine D1, D2, D3, D5 receptor subtype mRNAs and dopamine transporter mRNA were detected in both superior cervical sympathetic ganglia (SCG) and dorsal root ganglia (DRG) in the rat; the expression of D4 mRNA was only detected in DRG. While two alternatively spliced isoforms of D2 were detected in both ganglia, the alternative splicing transcripts for D3 and D4 were only found in the DRG. These results are useful in further studying the roles of dopamine and the effects of dopaminergic agents in the peripheral nervous system.
authors.affiliation: Spectra Biomedical, 4040 Campbell Ave., 2nd Floor, Menlo Park, CA 94025, USA.
authors.forename: G X
authors.initials: GX
authors.lastname: Xie
authors.forename: K
authors.initials: K
authors.lastname: Jones
authors.forename: S J
authors.initials: SJ
authors.lastname: Peroutka
authors.forename: P P
authors.initials: PP
authors.lastname: Palmer
mesh_terms: D017398:Alternative Splicing; D000818:Animals; D002352:Carrier Proteins / Q000096:biosynthesis; D017931:DNA Primers; D050483:Dopamine Plasma Membrane Transport Proteins; D005727:Ganglia, Spinal / Q000166:cytology / Q000378:metabolism*; D008297:Male; D008562:Membrane Glycoproteins; D026901:Membrane Transport Proteins; D009419:Nerve Tissue Proteins; D016133:Polymerase Chain Reaction / Q000379:methods; D012333:RNA, Messenger / Q000032:analysis / Q000096:biosynthesis*; D051381:Rats; D017207:Rats, Sprague-Dawley; D011954:Receptors, Dopamine / Q000096:biosynthesis*; D017447:Receptors, Dopamine D1 / Q000096:biosynthesis; D017448:Receptors, Dopamine D2 / Q000096:biosynthesis; D050637:Receptors, Dopamine D3; D050638:Receptors, Dopamine D4; D050641:Receptors, Dopamine D5; D017783:Superior Cervical Ganglion / Q000166:cytology / Q000378:metabolism*; D014158:Transcription, Genetic
chemical_list: D002352:Carrier Proteins; D017931:DNA Primers; D050483:Dopamine Plasma Membrane Transport Proteins; C493595:Drd3 protein, rat; C493601:Drd4 protein, rat; C493607:Drd5 protein, rat; D008562:Membrane Glycoproteins; D026901:Membrane Transport Proteins; D009419:Nerve Tissue Proteins; D012333:RNA, Messenger; D011954:Receptors, Dopamine; D017447:Receptors, Dopamine D1; D017448:Receptors, Dopamine D2; D050637:Receptors, Dopamine D3; D050638:Receptors, Dopamine D4; D050641:Receptors, Dopamine D5