title: [Affinity sorbents with tripeptide morpholine ligands for isolation of proteases].
abstract: New affine sorbents were synthesized involving tripeptide morpholides H-Ala-Ala-Leu-Mrp and H-D-Ala-Leu-Arg-Mrp as ligands that mimic substrates of subtilisin-like proteases and kallikrein, respectively. These were used for the isolation and purification of several proteases: trypsin, pepsin, alpha-chymotrypsin, thrombin, kallikrein, and termitase and were also efficient in the isolation of proteolytic enzymes from complex mixtures, such as the urine of children suffering from glomerulonephritis, hepatopancreas of Kamchatka crab, and dandelion roots. The ligands are competitive inhibitors of a number of proteases, and therefore, they were supposed to interact with the substrate binding sites in these enzymes.
authors.affiliation: Moscow State University, Chemistry Department, Russia.
authors.forename: A V
authors.initials: AV
authors.lastname: Kuznetsova
authors.forename: G N
authors.initials: GN
authors.lastname: Rudenskaia
authors.forename: A M
authors.initials: AM
authors.lastname: Bogacheva
authors.forename: T L
authors.initials: TL
authors.lastname: Voiushina
authors.forename: V M
authors.initials: VM
authors.lastname: Stepanov
mesh_terms: D000818:Animals; D002846:Chromatography, Affinity; D004591:Electrophoresis, Polyacrylamide Gel; D010450:Endopeptidases / Q000302:isolation & purification*; D006801:Humans; D008024:Ligands; D009025:Morpholines / Q000737:chemistry*; D009842:Oligopeptides / Q000138:chemical synthesis* / Q000737:chemistry; D012685:Sepharose / Q000031:analogs & derivatives / Q000737:chemistry*; D013379:Substrate Specificity
chemical_list: D008024:Ligands; D009025:Morpholines; D009842:Oligopeptides; D012685:Sepharose; D010450:Endopeptidases
vernacular_title: Affinnye sorbenty dlia vydeleniia proteinaz, soderzhashchie v kachestve ligandov morfolidy tripeptidov.