The contryphans, a D-tryptophan-containing family of Conus peptides: interconversion between conformers.
We previously characterized contryphan-R, a D-tryptophan-containing octapeptide from the venom of Conus radiatus. In this study, we present evidence that the contryphan family of peptides is widely distributed in venoms of the fish-hunting cone snails. We purified, synthesized and characterized contryphan-Sm from Conus stercusmuscarum venom, and obtained molecular evidence for the existence of a third peptide, contryphan-P from Conus purpurascens venom ducts. The sequences of these three contryphans showed identity in seven of eight amino acids and a conserved pattern of post-translational modification. We also demonstrate that contryphan-Sm equilibrates between two distinct conformational states.
Jacobsen
R
R
Department of Biology, University of Utah, Salt Lake City 84112, USA.
Jimenez
E C
EC
Grilley
M
M
Watkins
M
M
Hillyard
D
D
Cruz
L J
LJ
Olivera
B M
BM
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002851:Chromatography, High Pressure Liquid; D018076:DNA, Complementary; D008969:Molecular Sequence Data; D008974:Mollusca; D008978:Mollusk Venoms / Q000737:chemistry*; D010456:Peptides, Cyclic / Q000737:chemistry* / Q000235:genetics / Q000302:isolation & purification; D016133:Polymerase Chain Reaction; D011487:Protein Conformation; D011994:Recombinant Proteins / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification
D018076:DNA, Complementary; D008978:Mollusk Venoms; D010456:Peptides, Cyclic; D011994:Recombinant Proteins; C102355:contryphan