The determination of nandrolone and its metabolites in urine by gas chromatography-mass spectrometry.
The determination of nandrolone and its major metabolites in urine of a healthy volunteer is typically performed by fused-silica capillary column gas chromatography with electron impact quadropole mass spectrometry. Two well-known urinary metabolites of nandrolone, 19-norandrosterone and 19-norethiocholanolone, were isolated by XAD-2 adsorption from urine, eluted with methanol and separated into unconjugated and conjugated fractions. The conjugated fraction was hydrolyzed with beta-glucuronidase from Escherichia coli and the samples derivatized with MSTFA/ammonium iodide/dithioerythritol. Ion fragmentograms of the bis-trimethylsilyl derivatives of nandrolone and its metabolites displayed molecular ions of M+ = 420 and M(+) -15 = 405. Extraction yield and the minimum detection limit of nandrolone in urine were identified. Finally, excretion rates of nandrolone and its metabolites in urine were determined.
Ozer
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Department of Analytical Chemistry, Faculty of Pharmacy, Hacettepe University, Ankara, Turkey.
Temìzer
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A
D000328:Adult; D045930:Anabolic Agents / Q000493:pharmacokinetics / Q000652:urine*; D001711:Biotransformation; D008401:Gas Chromatography-Mass Spectrometry; D006801:Humans; D007202:Indicators and Reagents; D008297:Male; D009277:Nandrolone / Q000493:pharmacokinetics / Q000652:urine*
D045930:Anabolic Agents; D007202:Indicators and Reagents; D009277:Nandrolone