title: Characterization of L-arginine uptake by plasma membrane vesicles isolated from cultured pulmonary artery endothelial cells.
abstract: We investigated the mechanisms of [3H]-L-arginine transport via System Y+ using plasma membrane vesicles derived from cultured pulmonary artery endothelial cells. [3H]-L-arginine uptake into plasma membrane vesicles was Na-independent, sensitive to trans-stimulation, unaffected by proton-conducting ionophores, and selectively inhibited by cationic amino acids. Kinetic experiments performed over a wide range of substrate concentrations revealed only one population of L-arginine transporters with Km = 130 microM. To elucidate the driving force for L-arginine transport, we measured [3H]-L-arginine uptake by plasma membrane vesicles at different transmembrane ion gradients. Plasma membrane vesicles accumulated [3H]-L-arginine only when a membrane potential was imposed across the vesicles, and the velocity of uptake was linearly related to the magnitude of the created membrane potential. The presence of potassium ions inside the vesicles was not essential for uptake of L-arginine into vesicles, but it was essential for trans-stimulation of L-arginine transport. [3H]-L-arginine accumulated in plasma membrane vesicles can be released by agents that dissipate transmembrane potassium gradients (e.g. saponin, gramicidin, and nigericin). Diazoxide and pinacidil, activators of K(+)-channels, had no significant effect on [3H]-L-arginine uptake, whereas tetraethylammonium chloride, 4-aminopyridine, and glibenclamide, inhibitors of K(+)-channels, caused decreases in [3H]-L-arginine transport by plasma membrane vesicles. This study demonstrates for the first time a specific role for potassium ions in the mechanism of L-arginine transport, particularly in the phenomenon of trans-stimulation.
authors.affiliation: Research Service, VA Medical Center, Gainesville, FL, USA.
authors.forename: S I
authors.initials: SI
authors.lastname: Zharikov
authors.forename: E R
authors.initials: ER
authors.lastname: Block
mesh_terms: D000596:Amino Acids / Q000494:pharmacology; D000818:Animals; D001120:Arginine / Q000031:analogs & derivatives / Q000378:metabolism* / Q000494:pharmacology; D001692:Biological Transport; D002462:Cell Membrane / Q000187:drug effects / Q000378:metabolism; D002478:Cells, Cultured; D004730:Endothelium, Vascular / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D007476:Ionophores / Q000494:pharmacology; D007700:Kinetics; D026902:Potassium Channel Blockers; D015221:Potassium Channels / Q000819:agonists; D011651:Pulmonary Artery / Q000166:cytology / Q000378:metabolism*; D012964:Sodium / Q000378:metabolism; D013552:Swine; D014316:Tritium
chemical_list: D000596:Amino Acids; D007476:Ionophores; D026902:Potassium Channel Blockers; D015221:Potassium Channels; D014316:Tritium; D001120:Arginine; D012964:Sodium