Secretion of heterologous proteins from Schizosaccharomyces pombe using the homologous leader sequence of pho1+ acid phosphatase.
In this study we report the use of the S. pombe leader sequence of pho1+ acid phosphatase (Elliott et al., J. Biol. Chem. 216, 2916-2941, 1986) for the secretion of heterologous proteins into the medium. The green fluorescent protein (GFP) and the Human Papillomavirus (HPV) type 16 E7 protein are normally not secreted; fusion of the S. pombe pho1 leader peptide (SPL) to GFP and HPV 16 E7 resulted in an efficient secretion of these proteins although the latter contains a nuclear targeting sequence. These data suggest that SPL fused constructs could be applied for the production of other recombinant proteins using the S. pombe expression system. Furthermore, since GFP retains its intrinsic fluorescence during the secretion, this system may be useful to study the secretory pathway of fission yeast in vivo.
Braspenning
J
J
Deutsches Krebsforschungszentrum, Angewandte Tumorvirologie, INF, 242, 69120 Heidelberg, Germany. j.braspenning@dkfz-heidelberg.de
Meschede
W
W
Marchini
A
A
Müller
M
M
Gissmann
L
L
Tommasino
M
M
D000135:Acid Phosphatase / Q000737:chemistry*; D005453:Fluorescence; D005656:Fungal Proteins / Q000378:metabolism*; D049452:Green Fluorescent Proteins; D008164:Luminescent Proteins / Q000235:genetics / Q000378:metabolism; D008856:Microscopy, Fluorescence; D009856:Oncogene Proteins, Viral / Q000378:metabolism; D050725:Papillomavirus E7 Proteins; D010750:Phosphoproteins / Q000378:metabolism; D021382:Protein Sorting Signals / Q000235:genetics / Q000378:metabolism*; D011993:Recombinant Fusion Proteins / Q000302:isolation & purification / Q000378:metabolism; D012568:Schizosaccharomyces / Q000201:enzymology / Q000378:metabolism*; D014764:Viral Proteins / Q000378:metabolism
D005656:Fungal Proteins; D008164:Luminescent Proteins; D009856:Oncogene Proteins, Viral; D050725:Papillomavirus E7 Proteins; D010750:Phosphoproteins; D021382:Protein Sorting Signals; D011993:Recombinant Fusion Proteins; D014764:Viral Proteins; C059731:oncogene protein E7, Human papillomavirus type 16; D049452:Green Fluorescent Proteins; D000135:Acid Phosphatase