title: Cloning genomic DNA encoding apple polyphenol oxidase and comparison of the gene product in Escherichia coli and in apple.
abstract: Two PCR-amplified genomic DNA fragments encoding apple (cv. Fuji) polyphenol oxidase (PPO) were cloned and sequenced. A comparison of genomic DNA with cDNAs revealed that the PPOs lacked introns. Both PPO DNAs appear to encode a 66-kDa precursor protein consisting of a 56-kDa mature protein and a N-terminal transit peptide of 10-kDa N-terminal transit peptide. Apple PPO DNA was expressed in Escherichia coli, and the gene product (56 kDa) without a transit peptide was immunochemically detected and was the same size (ca. 65 kDa) as the main PPO of apple fruit by SDS-PAGE.
authors.affiliation: Department of Nutrition and Food Science, University of Tokyo, Japan.
authors.forename: M
authors.initials: M
authors.lastname: Haruta
authors.forename: M
authors.initials: M
authors.lastname: Murata
authors.forename: A
authors.initials: A
authors.lastname: Hiraide
authors.forename: H
authors.initials: H
authors.lastname: Kadokura
authors.forename: M
authors.initials: M
authors.lastname: Yamasaki
authors.forename: M
authors.initials: M
authors.lastname: Sakuta
authors.forename: S
authors.initials: S
authors.lastname: Shimizu
authors.forename: S
authors.initials: S
authors.lastname: Homma
mesh_terms: D000595:Amino Acid Sequence; D015139:Blotting, Southern; D015153:Blotting, Western; D004156:Catechol Oxidase / Q000096:biosynthesis / Q000737:chemistry / Q000235:genetics*; D003001:Cloning, Molecular; D004591:Electrophoresis, Polyacrylamide Gel; D004926:Escherichia coli / Q000201:enzymology* / Q000235:genetics; D015964:Gene Expression Regulation, Bacterial; D007438:Introns; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D019685:Rosales / Q000201:enzymology*; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid
chemical_list: D004156:Catechol Oxidase