Higher sensitivity in induction of apoptosis in fibroblast cell lines derived from LEC strain rats to UV-irradiation.
The proportion of S-phase cells in a WKAH rat cell population decreased and that of G1-phase cells increased at 8 and 18 hr post-incubation following UV-irradiation, although no significant change was observed in the ratio of the proportion of S-phase to G1-phase cells in a LEC rat cell population. Thus, UV-radiation-induced delay in the progression from the G1 to S phase was observed in WKAH rat cells but was not apparent in LEC rat cells. The fraction of LEC rat cells containing a sub-G0 DNA content increased with post-incubation time after UV-irradiation, but not that of WKAH rat cells. The proportion of the sub-G0 fraction in LEC rat cells increased with increasing doses of UV-rays. Low molecular weight DNAs extracted from UV-irradiated LEC rat cells exhibited an intense DNA ladder pattern at 18 and 24 hr post-irradiation by electrophoretic analysis, but not those from UV-irradiated WKAH rat cells. These results showed a higher sensitivity of LEC rat cells in induction of apoptosis than that of WKAH rat cells to UV-irradiation, although there was no difference in the survival curves among the cell lines from LEC and WKAH rats after UV-irradiation.
Hayashi
M
M
Department of Veterinary Radiology, Faculty of Veteriniary Medicine, Rakuno Gakuen University, Ebetsu, Japan.
Uehara
K
K
Ichikawa
Y
Y
Arai
S
S
Isogai
E
E
Okui
T
T
D000818:Animals; D017209:Apoptosis / Q000502:physiology / Q000528:radiation effects*; D002453:Cell Cycle / Q000502:physiology / Q000528:radiation effects*; D002460:Cell Line; D002470:Cell Survival / Q000528:radiation effects; D004307:Dose-Response Relationship, Radiation; D005347:Fibroblasts / Q000166:cytology / Q000502:physiology / Q000528:radiation effects; D016193:G1 Phase; D007700:Kinetics; D051381:Rats; D011919:Rats, Inbred Strains; D016196:S Phase; D013045:Species Specificity; D013997:Time Factors; D014466:Ultraviolet Rays