title: Genes coding for respiratory complexes map on all three chromosomes of the Paracoccus denitrificans genome.
abstract: The genome of Paracoccus denitrificans (strain Pd1222) consists of three distinct DNA molecules when separated by standard pulsed-field gel electrophoresis with apparent molecular sizes of approximately 2, 1.1, and 0.64 Mb. When the separated chromosomes are digested by restriction enzymes and sizes of resulting fragments are summed up, the three chromosomes are composed of 1.83, 1.16, and 0.67 Mb. Since their migration behavior relative to size standards is largely independent of electrophoresis conditions, at least the two smaller chromosomes most likely represent linear molecules. The size analysis presented here allows an unequivocal distinction between groups of different strains of P. denitrificans and of Thiosphaera pantotropha, confirming an earlier cytochrome c analysis. When the genome was analyzed with different probes coding for respiratory enzymes, essential genes were found spread over all three chromosomes without any obvious clustering on any of the three forms.
authors.affiliation: Molecular Genetics, Institute of Biochemistry, Biozentrum, Marie-Curie-Strasse 9, D-60439 Frankfurt, Germany.
authors.forename: C
authors.initials: C
authors.lastname: Winterstein
authors.forename: B
authors.initials: B
authors.lastname: Ludwig
mesh_terms: D002874:Chromosome Mapping; D002876:Chromosomes, Bacterial / Q000201:enzymology / Q000235:genetics*; D004269:DNA, Bacterial / Q000032:analysis / Q000235:genetics; D004579:Electron Transport / Q000235:genetics*; D014450:Electron Transport Complex III / Q000235:genetics; D003576:Electron Transport Complex IV / Q000032:analysis / Q000235:genetics; D016521:Electrophoresis, Gel, Pulsed-Field; D005798:Genes, Bacterial / Q000235:genetics*; D005819:Genetic Markers / Q000235:genetics; D016680:Genome, Bacterial; D010231:Paracoccus denitrificans / Q000201:enzymology / Q000235:genetics*; D012150:Polymorphism, Restriction Fragment Length
chemical_list: D004269:DNA, Bacterial; D005819:Genetic Markers; C038862:cytochrome o oxidase; D003576:Electron Transport Complex IV; D014450:Electron Transport Complex III