Deletion analysis of human AP-endonuclease: minimum sequence required for the endonuclease activity.
AP-endonuclease (APE) plays a central role in the base excision repair process for oxidative and alkylation damage in DNA. The major human APE with 318 amino acid residues possesses transcriptional regulatory activities for which the N-terminal region was found to be essential. Systematic deletion studies of both amino and carboxyl termini of the human APE have shown that the carboxyl termini residues are essential for the endonuclease activity as indicated by direct measurement of enzyme activity and from studies on phenotypic rescue of Escherichia coli. However, the amino-terminal residues are dispensable for this activity and the boundary of the active endonuclease lies at this end between positions 61 and 80.
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Sealy Center for Molecular Science and Department of Human Biological Chemistry and Genetics, University of Texas Medical Branch, Galveston 77555-1079, USA.
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D019757:Carbon-Oxygen Lyases / Q000235:genetics / Q000378:metabolism*; D004249:DNA Damage; D004260:DNA Repair; D043603:DNA-(Apurinic or Apyrimidinic Site) Lyase; D043223:Deoxyribonuclease IV (Phage T4-Induced); D004926:Escherichia coli / Q000235:genetics; D029968:Escherichia coli Proteins; D006801:Humans; D010084:Oxidation-Reduction; D010641:Phenotype; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism; D017384:Sequence Deletion; D014158:Transcription, Genetic
D029968:Escherichia coli Proteins; D011994:Recombinant Proteins; D043223:Deoxyribonuclease IV (Phage T4-Induced); C468972:endonuclease IV, E coli; D019757:Carbon-Oxygen Lyases; D043603:DNA-(Apurinic or Apyrimidinic Site) Lyase