Phylogenetic position of an obligately chemoautotrophic, marine hydrogen-oxidizing bacterium, Hydrogenovibrio marinus, on the basis of 16S rRNA gene sequences and two form I RuBisCO gene sequences.
Two form ribulose-1,5-bisphosphate carboxylase/oxygenase (RuBisCO) genes from the obligately autotrophic, marine hydrogen oxidizer Hydrogenovibrio marinus were sequenced. The deduced amino acid sequences of both RuBisCOs revealed that they are similar to those of sulfur oxidizers (Thiobacillus) and a purple sulfur bacterium (Chromatium vinosum). According to the 16S rRNA gene sequences, H. marinus is also affiliated with these microorganisms, members of Thiomicrospira being the closest relatives. Sequence similarities of the 16S rRNA genes and of the RuBisCO genes among these gamma-Proteobacteria suggest a common autotrophic ancestry. An ancestor of purple sulfur bacteria might be a common root of H. marinus and related sulfur oxidizers.
Nishihara
H
H
Department of Biotechnology, University of Tokyo, Bunkyo-ku, Tokyo 113, Japan. aigara@hongo.ecc.u-tokyo.ac.jp
Yaguchi
T
T
Chung
S Y
SY
Suzuki
K
K
Yanagi
M
M
Yamasato
K
K
Kodama
T
T
Igarashi
Y
Y
D001426:Bacterial Proteins / Q000235:genetics; D002874:Chromosome Mapping; D004275:DNA, Ribosomal / Q000235:genetics; D004926:Escherichia coli / Q000235:genetics; D005798:Genes, Bacterial / Q000235:genetics*; D006859:Hydrogen / Q000378:metabolism; D007527:Isoenzymes / Q000235:genetics*; D008386:Marine Biology; D008969:Molecular Sequence Data; D008970:Molecular Weight; D010084:Oxidation-Reduction; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics*; D011994:Recombinant Proteins / Q000032:analysis / Q000235:genetics; D012273:Ribulose-Bisphosphate Carboxylase / Q000032:analysis / Q000235:genetics*; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D014169:Transformation, Bacterial / Q000235:genetics; D014737:Vibrionaceae / Q000737:chemistry / Q000201:enzymology / Q000235:genetics*; D014871:Water Microbiology
D001426:Bacterial Proteins; D004275:DNA, Ribosomal; D007527:Isoenzymes; D012336:RNA, Ribosomal, 16S; D011994:Recombinant Proteins; D006859:Hydrogen; D012273:Ribulose-Bisphosphate Carboxylase