Induction of UCP2 gene expression by LPS: a potential mechanism for increased thermogenesis during infection.
UCP2 has been proposed to regulate thermogenesis and energy expenditure. To identify potential mechanisms underlying the increased energy expenditure and heat production during infection, we investigated whether LPS and cytokines might increase UCP2 mRNA levels in mice. LPS (100 micrograms, i.p.) increased the expression of UCP2 mRNA in liver (28-fold) and muscle and white adipose tissue (5-fold). In liver, both IL-1 beta (1 microgram, i.p.) and TNF (5 micrograms, i.p.) increased UCP2 mRNA levels, 4- and 3-fold respectively, whereas in muscle and fat tissue, an increase was detectable after TNF, but not IL-1 beta. Indomethacin (10 mg/kg, i.p.) administered immediately before LPS markedly reduced (70%) the ability of LPS to increase UCP2 mRNA in liver, but not in muscle or adipose tissue. These results suggest a role for UCP2 in the heat production and increased energy expenditure that occurs during infection.
Faggioni
R
R
Department of Medicine, University of California, San Francisco, USA.
Shigenaga
J
J
Moser
A
A
Feingold
K R
KR
Grunfeld
C
C
D000818:Animals; D001833:Body Temperature Regulation / Q000187:drug effects / Q000235:genetics*; D004927:Escherichia coli Infections / Q000235:genetics* / Q000503:physiopathology; D005260:Female; D005786:Gene Expression Regulation / Q000187:drug effects*; D007213:Indomethacin / Q000008:administration & dosage; D007274:Injections, Intraperitoneal; D007375:Interleukin-1 / Q000096:biosynthesis / Q000378:metabolism; D007473:Ion Channels; D007700:Kinetics; D008070:Lipopolysaccharides / Q000008:administration & dosage*; D008099:Liver / Q000378:metabolism; D026901:Membrane Transport Proteins; D051379:Mice; D008810:Mice, Inbred C57BL; D008928:Mitochondria / Q000378:metabolism*; D024101:Mitochondrial Proteins; D011453:Prostaglandins / Q000502:physiology; D014176:Protein Biosynthesis; D011506:Proteins / Q000235:genetics*; D012333:RNA, Messenger / Q000096:biosynthesis; D014409:Tumor Necrosis Factor-alpha / Q000096:biosynthesis / Q000378:metabolism; D000071246:Uncoupling Protein 2
D007375:Interleukin-1; D007473:Ion Channels; D008070:Lipopolysaccharides; D026901:Membrane Transport Proteins; D024101:Mitochondrial Proteins; D011453:Prostaglandins; D011506:Proteins; D012333:RNA, Messenger; D014409:Tumor Necrosis Factor-alpha; C000604390:Ucp2 protein, mouse; D000071246:Uncoupling Protein 2; D007213:Indomethacin