title: Microscopic analysis of NADH fluorescence during aerobic and anaerobic liver preservation conditions: A noninvasive technique for assessment of hepatic metabolism.
abstract: Gaseous insufflation of oxygen via the venous vascular system is thought to be an useful tool for preventing anoxic tissue injury during extended time periods of ischemic preservation and for allowing for an improved recovery of organ function after transplantation. The present study aimed at the application of a noninvasive technique for monitoring effectiveness and homogeneity of gaseous areation by using an epiillumination microscopic technique for assessment of tissue nicotinamide adenine dinucleotide (NADH) fluorescence. Rat livers were flushed with and stored in University of Wisconsin solution at 4 degrees C for 48 h (n = 20). In half of the experiments (n = 10) gaseous oxygen was applied subsequent to organ harvest. Using ultraviolet-excitation high-resolution microscopy and computer-assisted image analysis liver surfaces were scanned for NADH intensity and spatial heterogeneity at 1, 24, and 48 h preservation time. Livers simply stored without aeration served as controls (n = 10). NADH intensity data were compared with corresponding data of tissue adenosine triphosphate (ATP) concentrations determined enzymatically. NADH fluorescence already differed at 1 h preservation between the two groups with significantly lower values in the aerobically stored livers. NADH fluorescence further decreased between 1 and 24 h preservation and remained low until 48 h, whereas in the anaerobically stored livers NADH fluorescence was found to be constantly high over the entire observation period. Aerobic storage resulted in rather homogeneous tissue oxygenation with an intrahepatic variation of NADH fluorescence <20%. In parallel, oxygen persufflation appropriately restored tissue ATP content within 1 to 24 h of preservation, while the simply stored livers exhibited pronounced depletion of ATP. We demonstrate for the first time that by means of retrograde gaseous oxygenation, ischemic livers can be readily and effectively oxygenated. Our study further indicates that the noninvasive microscopic analysis of tissue NADH fluorescence may be an useful tool for estimating efficiency of strategies in organ preservation.
authors.affiliation: Institute for Experimental Medicine, University of Cologne, Cologne, 50931, Germany.
authors.forename: H
authors.initials: H
authors.lastname: Klauke
authors.forename: T
authors.initials: T
authors.lastname: Minor
authors.forename: B
authors.initials: B
authors.lastname: Vollmar
authors.forename: W
authors.initials: W
authors.lastname: Isselhard
authors.forename: M D
authors.initials: MD
authors.lastname: Menger
mesh_terms: D000241:Adenosine; D000332:Aerobiosis; D000493:Allopurinol; D000693:Anaerobiosis; D000818:Animals; D005453:Fluorescence; D005978:Glutathione; D007328:Insulin; D008099:Liver; D009243:NAD / Q000032:analysis*; D009926:Organ Preservation; D019188:Organ Preservation Solutions; D010100:Oxygen; D011887:Raffinose; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D007328:Insulin; D019188:Organ Preservation Solutions; C055206:University of Wisconsin-lactobionate solution; D009243:NAD; D000493:Allopurinol; D005978:Glutathione; D000241:Adenosine; D011887:Raffinose; D010100:Oxygen