cDNA cloning and expression analysis of the murine ribonuclease L inhibitor.
The 2-5A/RNase L system is one of the pathways induced by interferon (IFN). It plays a major role in the antiviral and antiproliferative activities of IFNs. Recently, we have shown that the activity of the RNase L could be inhibited by a proteic inhibitor, the RNase L Inhibitor (RLI). Human RLI (Hu-RLI) was cloned and characterized. We describe here the isolation and characterization of the cDNA encoding the murine RLI (Mu-RLI). Hu-RLI and Mu-RLI protein have 98% amino acid identity. Mu-RLI is functionally homologous to Hu-RLI, and all the structural features and amino acid sequence motifs of Hu-RLI are conserved in Mu-RLI. Moreover, reticulocyte lysate translated Mu-RLI protein is also able to inhibit 2-5A binding on 2-5A-dependent RNAse-L. Northern blot analysis revealed that Mu-RLI cDNA hybridizes with one mRNA of 3.5 kb except for the testis where two mRNA of 3.5 and 2.1 kb, respectively, are detected, suggesting a tissue-specific regulation.
Benoit De Coignac
A
A
IGMM-CNRS-UMR 5535, 1919 route de Mende, 34293, Montpellier Cedex 5, France.
Bisbal
C
C
Lebleu
B
B
Salehzada
T
T
D018528:ATP-Binding Cassette Transporters; D000227:Adenine Nucleotides / Q000378:metabolism; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D001667:Binding, Competitive; D018833:Chaperonins; D003001:Cloning, Molecular; D017124:Conserved Sequence; D018076:DNA, Complementary; D004791:Enzyme Inhibitors / Q000737:chemistry* / Q000494:pharmacology; D006801:Humans; D008297:Male; D017017:Methanococcus / Q000235:genetics; D051379:Mice; D008969:Molecular Sequence Data; D009843:Oligoribonucleotides / Q000378:metabolism; D009928:Organ Specificity; D014176:Protein Biosynthesis; D011506:Proteins / Q000737:chemistry* / Q000494:pharmacology; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000737:chemistry / Q000494:pharmacology; D012441:Saccharomyces cerevisiae / Q000235:genetics; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid; D014158:Transcription, Genetic
C501917:ABCE1 protein, human; D018528:ATP-Binding Cassette Transporters; C501918:Abce1 protein, mouse; D000227:Adenine Nucleotides; D018076:DNA, Complementary; D004791:Enzyme Inhibitors; D009843:Oligoribonucleotides; D011506:Proteins; D011994:Recombinant Proteins; C023505:2',5'-oligoadenylate; D018833:Chaperonins