title: Viremia in chronic hepatitis C patients evaluated by the Amplicor RT-PCR, a nested RT-PCR, and transaminase levels.
abstract: A commercially available kit, Amplicor, was compared with a locally developed nested reverse-transcriptase (RT) PCR for qualitative detection of HCV-RNA. Sixty-one serum samples from sixty-one patients with liver disease, and 60 samples from 60 hemophiliacs without symptoms, but known to have been heavily exposed to hepatitis C virus, were investigated. There was a high degree of concordance between the two diagnostic tests (97%), the Amplicor kit being slightly more sensitive than the in-house PCR, when evaluated using serial dilutions of samples showing discrepant results. The relationship between viremia and abnormal ALT levels was studied in the two groups of patients. Among those with chronic liver disease, 8.3% of patients with viremia had normal ALT levels, whereas transaminases were normal in 20% of hemophiliacs with viremia. This points to ALT as being a poor marker of ongoing viral replication.
authors.affiliation: Department of Infectious Diseases, Marselisborg Hospital, University of Aarhus, Denmark.
authors.forename: A L
authors.initials: AL
authors.lastname: Laursen
authors.forename: J
authors.initials: J
authors.lastname: Ingerslev
authors.forename: P L
authors.initials: PL
authors.lastname: Andersen
authors.forename: L
authors.initials: L
authors.lastname: Ostergaard
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000410:Alanine Transaminase / Q000097:blood*; D005260:Female; D016174:Hepacivirus / Q000235:genetics*; D019698:Hepatitis C, Chronic / Q000175:diagnosis / Q000821:virology*; D006801:Humans; D008297:Male; D008875:Middle Aged; D016133:Polymerase Chain Reaction / Q000379:methods*; D012194:RNA-Directed DNA Polymerase; D011933:Reagent Kits, Diagnostic; D014766:Viremia / Q000175:diagnosis / Q000821:virology*
chemical_list: D011933:Reagent Kits, Diagnostic; D000410:Alanine Transaminase; D012194:RNA-Directed DNA Polymerase