The yeast gene YJR025c encodes a 3-hydroxyanthranilic acid dioxygenase and is involved in nicotinic acid biosynthesis.
We have deleted the yeast gene YJR025c and shown that this leads to an auxotrophy for nicotinic acid. The deduced protein sequence of the gene product is homologous to the human 3-hydroxyanthranilic acid dioxygenase (EC 1.13.11.6) which is part of the kynurenine pathway for the degradation of tryptophan and the biosynthesis of nicotinic acid. In cell-free extracts the 3-hydroxyanthranilic acid dioxygenase activity is proportional to the copy number of the YJR025c gene. As YJR025c encodes the yeast 3-hydroxyanthranilic acid dioxygenase, we have named this gene BNA1 for biosynthesis of nicotinic acid.
Kucharczyk
R
R
Institute of Biochemistry and Biophysics, Polish National Academy of Sciences, Warsaw.
Zagulski
M
M
Rytka
J
J
Herbert
C J
CJ
D050561:3-Hydroxyanthranilate 3,4-Dioxygenase; D001483:Base Sequence; D049308:Dioxygenases; D017353:Gene Deletion; D005800:Genes, Fungal; D005816:Genetic Complementation Test; D007737:Kynurenine / Q000378:metabolism; D008969:Molecular Sequence Data; D009525:Niacin / Q000096:biosynthesis* / Q000378:metabolism; D010105:Oxygenases / Q000235:genetics* / Q000378:metabolism*; D012441:Saccharomyces cerevisiae / Q000235:genetics* / Q000378:metabolism*; D029701:Saccharomyces cerevisiae Proteins; D014364:Tryptophan / Q000378:metabolism
D029701:Saccharomyces cerevisiae Proteins; D009525:Niacin; D007737:Kynurenine; D014364:Tryptophan; D010105:Oxygenases; D049308:Dioxygenases; D050561:3-Hydroxyanthranilate 3,4-Dioxygenase; C493361:BNA1 protein, S cerevisiae