Isolation and characterization of the dnaKJ operon from Actinobacillus actinomycetemcomitans.
The dnaKJ operon of Actinobacillus actinomycetemcomitans Y4 was cloned by the DNA-probing method using synthetic oligonucleotides designed on the basis of two conserved regions in DnaK/hsp70 proteins and sequenced by inverse PCR. The sequenced region was shown to contain two open reading frames coding for proteins analogous to DnaK and DnaJ.
Yoshida
A
A
First Department of Oral and Maxillofacial Surgery, Kyushu University Faculty of Dentistry, Fukuoka, Japan.
Nakano
Y
Y
Yamashita
Y
Y
Yu
H
H
Ohishi
M
M
Koga
T
T
D016976:Aggregatibacter actinomycetemcomitans / Q000235:genetics*; D000595:Amino Acid Sequence; D001483:Base Sequence; D003001:Cloning, Molecular; D029968:Escherichia coli Proteins; D050956:HSP40 Heat-Shock Proteins; D018840:HSP70 Heat-Shock Proteins / Q000235:genetics*; D006360:Heat-Shock Proteins / Q000235:genetics*; D008969:Molecular Sequence Data; D009876:Operon; D017421:Sequence Analysis; D017386:Sequence Homology, Amino Acid
C494635:DnaJ protein, E coli; D029968:Escherichia coli Proteins; D050956:HSP40 Heat-Shock Proteins; D018840:HSP70 Heat-Shock Proteins; D006360:Heat-Shock Proteins; C063842:dnaK protein, E coli