A pumpkin 72-kDa membrane protein of precursor-accumulating vesicles has characteristics of a vacuolar sorting receptor.
Precursor-accumulating (PAC) vesicles were previously shown to mediate the transport of the precursor of a major storage protein (pro2S albumin) to protein-storage vacuoles in developing pumpkin cotyledons. In this study, we characterized two homologous proteins from PAC vesicles, a 72 kDa protein (PV72) and an 82 kDa protein (PV82). PV72 and PV82 showed an ability to bind to peptides derived from both an internal propeptide and a C-terminal peptide of pro2S albumin. PV72 was predicted to be a type I integral membrane protein with epidermal growth factor (EGF)-like motifs. These results suggest that PV72 and PV82 are potential sorting receptors for 2S albumin to protein-storage vacuoles.
Shimada
T
T
Department of Cell Biology, National Institute for Basic Biology, Okazaki, Japan.
Kuroyanagi
M
M
Nishimura
M
M
Hara-Nishimura
I
I
D055516:2S Albumins, Plant; D000418:Albumins / Q000378:metabolism; D000595:Amino Acid Sequence; D052179:Antigens, Plant; D001692:Biological Transport; D019662:Cucurbitaceae / Q000235:genetics / Q000378:metabolism*; D018076:DNA, Complementary; D008565:Membrane Proteins / Q000235:genetics / Q000378:metabolism*; D008969:Molecular Sequence Data; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism*; D011485:Protein Binding; D011498:Protein Precursors / Q000378:metabolism; D018160:Receptors, Cytoplasmic and Nuclear / Q000235:genetics / Q000378:metabolism*; D017386:Sequence Homology, Amino Acid; D014617:Vacuoles / Q000378:metabolism
D055516:2S Albumins, Plant; D000418:Albumins; D052179:Antigens, Plant; D018076:DNA, Complementary; D008565:Membrane Proteins; C111597:PAC vesicle 72-kDa protein, Cucurbita; D010940:Plant Proteins; D011498:Protein Precursors; D018160:Receptors, Cytoplasmic and Nuclear