title: Stability of undiluted and diluted adenosine at three temperatures in syringes and bags.
abstract: The stability of adenosine in various diluents in polypropylene syringes and polyvinyl chloride (PVC) bags at three temperatures was studied. Portions of pooled undiluted adenosine infusion (3 mg/ mL) were stored in 60-mL capped syringes, 20 for each storage condition. Adenosine infusions were prepared by mixing adenosine with 5% dextrose injection, 0.9% sodium chloride injection, lactated Ringer's injection, or 5% dextrose and lactated Ringer's injection to produce a concentration of 0.75 mg/mL. Samples of each infusion were stored in 60-mL capped syringes and 50-mL bags, 20 syringes and 20 bags for each storage condition. Syringes and bags were stored in the dark at 25, 5, and -15 degrees C. At various sampling times, three syringes and three bags of each infusion were removed for visual inspection, pH measurement, and high-performance liquid chromatographic analysis. At 10 and 16 days, fungal growth at 25 degrees C was suspected in the infusions prepared with 5% dextrose injection. For all other samples, there was no evidence of precipitation or change in pH. The concentration of adenosine remained constant in all samples at all storage conditions. Adenosine 3 mg/mL was stable in polypropylene syringes for 7 days at 25 degrees C, 14 days at 5 degrees C, and 28 days at -15 degrees C; adenosine 0.75 mg/ mL in 0.9% sodium chloride injection and in 5% dextrose injection was stable in polypropylene syringes and PVC bags for 16 days at 25, 5, and -15 degrees C; and adenosine 0.75 mg/mL in lactated Ringer's injection and in 5% dextrose and lactated Ringer's injection was stable in syringes and bags for 14 days at 25, 5, and -15 degrees C.
authors.affiliation: College of Pharmacy, University of Iowa, Iowa City 52242-1112, USA.
authors.forename: V A
authors.initials: VA
authors.lastname: Ketkar
authors.forename: W M
authors.initials: WM
authors.lastname: Kolling
authors.forename: N
authors.initials: N
authors.lastname: Nardviriyakul
authors.forename: K
authors.initials: K
authors.lastname: VanDer Kamp
authors.forename: D E
authors.initials: DE
authors.lastname: Wurster
mesh_terms: D000241:Adenosine / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid; D004349:Drug Packaging; D004355:Drug Stability; D004356:Drug Storage; D006863:Hydrogen-Ion Concentration; D019999:Pharmaceutical Solutions / Q000032:analysis; D013594:Syringes; D013696:Temperature; D013997:Time Factors; D014665:Vasodilator Agents / Q000032:analysis*
chemical_list: D019999:Pharmaceutical Solutions; D014665:Vasodilator Agents; D000241:Adenosine