Isolation of a cDNA encoding mouse DNA topoisomerase III which is highly expressed at the mRNA level in the testis.
A cDNA (mTOP3) encoding mouse DNA topoisomerase III (topo III) was cloned using the human TOP3 cDNA as a probe. The deduced amino acid sequence of mouse topo III showed 86.8% identity with that of human topo III. Mouse TOP3 mRNA was highly expressed in the testis in comparison with other tissues. The TOP3 mRNA level in the testis increased slightly 14 days after birth and showed a marked increase in 17 days, times when the cells in pachytene phase begin to appear and increase in number.
Seki
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Department of Physiological Chemistry, Graduate School of Pharmaceutical Sciences, University of Tokyo, Japan.
Seki
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Katada
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Enomoto
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D000367:Age Factors; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D004264:DNA Topoisomerases, Type I / Q000235:genetics*; D018076:DNA, Complementary / Q000302:isolation & purification; D005260:Female; D006801:Humans; D007231:Infant, Newborn; D008297:Male; D051379:Mice; D008969:Molecular Sequence Data; D012333:RNA, Messenger / Q000378:metabolism; D012689:Sequence Homology, Nucleic Acid; D013737:Testis / Q000378:metabolism*; D014018:Tissue Distribution
D018076:DNA, Complementary; D012333:RNA, Messenger; D004264:DNA Topoisomerases, Type I