Dendritic cells fused with mastocytoma cells elicit therapeutic antitumor immunity.
Characterization of the spontaneous immune response that frequently occurs in tumor-bearing animals, as well as immunization using dendritic cells pulsed with tumor antigens, suggests that a limiting factor of the tumor-specific immune response may be a defect in the co-stimulatory signal that is required for optimal activation of T cells. In this work, we describe a new approach to improve the antigen-presenting capacity of tumor cells, which does not require a source of purified tumor-associated antigen. We fused P815 mastocytoma cells with bone marrow-derived dendritic cells. We obtained one hybrid that displayed the phenotypic and functional properties of dendritic cells and expressed mRNA coding for the tumor-associated antigen P815 A/B. Injections of irradiated hybrid cells prevented the growth of preimplanted mastocytoma and induced long-lasting tumor resistance.
Lespagnard
L
L
Départment of Molecular Biology, Université Libre de Bruxelles, Rhode-Saint-Genèse, Belgium.
Mettens
P
P
Verheyden
A M
AM
Tasiaux
N
N
Thielemans
K
K
van Meirvenne
S
S
Geldhof
A
A
De Baetselier
P
P
Urbain
J
J
Leo
O
O
Moser
M
M
D000818:Animals; D001854:Bone Marrow Cells / Q000187:drug effects / Q000276:immunology; D002459:Cell Fusion; D003713:Dendritic Cells / Q000166:cytology / Q000276:immunology*; D005260:Female; D016178:Granulocyte-Macrophage Colony-Stimulating Factor / Q000494:pharmacology; D016219:Immunotherapy, Adoptive; D012515:Mast-Cell Sarcoma / Q000276:immunology* / Q000473:pathology / Q000517:prevention & control*; D051379:Mice; D008808:Mice, Inbred CBA; D008811:Mice, Inbred DBA; D010641:Phenotype; D012333:RNA, Messenger / Q000378:metabolism; D014407:Tumor Cells, Cultured; D014409:Tumor Necrosis Factor-alpha / Q000494:pharmacology
D012333:RNA, Messenger; D014409:Tumor Necrosis Factor-alpha; D016178:Granulocyte-Macrophage Colony-Stimulating Factor