title: Characterization of ether-à-go-go channels present in photoreceptors reveals similarity to IKx, a K+ current in rod inner segments.
abstract: In this study, we describe two splice variants of an ether-à-go-go (EAG) K+ channel cloned from bovine retina: bEAG1 and bEAG2. The bEAG2 polypeptide contains an additional insertion of 27 amino acids in the extracellular linker between transmembrane segments S3 and S4. The heterologously expressed splice variants differ in their activation kinetics and are differently modulated by extracellular Mg2+. Cooperativity of modulation by Mg2+ suggests that each subunit of the putative tetrameric channel binds a Mg2+ ion. The channels are neither permeable to Ca2+ ions nor modulated by cyclic nucleotides. In situ hybridization localizes channel transcripts to photoreceptors and retinal ganglion cells. Comparison of EAG currents with IKx, a noninactivating K+ current in the inner segment of rod photoreceptors, reveals an intriguing similarity, suggesting that EAG polypeptides are involved in the formation of Kx channels.
authors.affiliation: Institut für Biologische Informationsverarbeitung, Forschungszentrum Jülich, Germany.
authors.forename: S
authors.initials: S
authors.lastname: Frings
authors.forename: N
authors.initials: N
authors.lastname: Brüll
authors.forename: C
authors.initials: C
authors.lastname: Dzeja
authors.forename: A
authors.initials: A
authors.lastname: Angele
authors.forename: V
authors.initials: V
authors.lastname: Hagen
authors.forename: U B
authors.initials: UB
authors.lastname: Kaupp
authors.forename: A
authors.initials: A
authors.lastname: Baumann
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D002118:Calcium / Q000378:metabolism; D002417:Cattle; D004553:Electric Conductivity; D051638:Ether-A-Go-Go Potassium Channels; D007536:Isomerism; D007700:Kinetics; D008274:Magnesium / Q000502:physiology; D008969:Molecular Sequence Data; D009712:Nucleotides, Cyclic / Q000502:physiology; D010539:Permeability; D010786:Photoreceptor Cells / Q000378:metabolism*; D011188:Potassium / Q000502:physiology*; D015221:Potassium Channels / Q000235:genetics* / Q000378:metabolism* / Q000502:physiology; D024642:Potassium Channels, Voltage-Gated; D012333:RNA, Messenger / Q000378:metabolism; D017948:Retinal Rod Photoreceptor Cells / Q000378:metabolism*; D017386:Sequence Homology, Amino Acid; D014018:Tissue Distribution
chemical_list: D051638:Ether-A-Go-Go Potassium Channels; C110930:KCNH1 protein, Bos taurus; D009712:Nucleotides, Cyclic; D015221:Potassium Channels; D024642:Potassium Channels, Voltage-Gated; D012333:RNA, Messenger; D008274:Magnesium; D011188:Potassium; D002118:Calcium