Protein C activation by recombinant thrombomodulin in plasma.
Recombinant glycosaminoglycan-modified urinary thrombomodulin (GAG-UTM), which partially improved the amino acid sequence of human urinary thrombomodulin (UTM), was expressed in C127 cells. GAG-UTM accelerates protein C activation by thrombin and also thrombin inhibition by antithrombin III (ATIII) in the buffer system. Both accelerating activities of GAG-UTM are more potent than those of unmodified recombinant UTM (r-UTM) without a GAG chain. As ATIII in plasma also inhibits protein C activation by a thrombin-thrombomodulin complex, we studied whether GAG-UTM accelerates protein C activation in plasma. GAG-UTM suppressed the generation of thrombin in activating plasma protein C stronger than r-UTM. By Western blot analysis using anti-protein C antibody, activated protein C was generated by GAG-UTM more than by r-UTM. From these results, the acceleration of activated protein C formation by GAG-UTM was confirmed in plasma too.
Edano
T
T
Tokyo Research Laboratories, Kowa Co., Ltd., Japan.
Komine
N
N
Yoshizaki
H
H
Ohkuchi
M
M
D015153:Blotting, Western; D002460:Cell Line; D006801:Humans; D008823:Micelles; D011486:Protein C / Q000378:metabolism*; D011994:Recombinant Proteins / Q000494:pharmacology; D013917:Thrombin / Q000096:biosynthesis / Q000378:metabolism; D018180:Thrombomodulin
D008823:Micelles; D011486:Protein C; D011994:Recombinant Proteins; D018180:Thrombomodulin; D013917:Thrombin