Brain type I but not type II IL-1 receptors mediate the effects of IL-1 beta on behavior in mice.
In the immune system, interleukin (IL)-1 beta effects are mediated by the type I IL-1 receptors (IL-1RI), whereas the type II IL-1 receptors (IL-1RII) act as inhibitory receptors. IL-1RI and IL-1RII are also present in the brain. To study their functionality in the brain, mice were centrally treated with neutralizing monoclonal antibody (MAb) directed against IL-1RI (35F5, 1 microgram) or against IL-1RII (4E2, 2 micrograms) and were centrally injected with recombinant rat IL-1 beta at a dose (2 ng) that decreased social exploration. Only 35F5 was effective in abrogating the behavioral effect of IL-1 beta. Moreover, 4E2 (1 microgram i.c.v.) did not potentiate the behavioral response to a subthreshold dose of IL-1 beta (1 ng i.c.v.). To examine the ability of brain IL-1RI to mediate the effects of endogenous IL-1 beta, mice were centrally treated with 35F5 (4 micrograms) and peripherally injected with IL-1 beta (1 microgram). Like IL-1 receptor antagonist (4 micrograms i.c.v.), 35F5 abrogated the effects of IL-1 beta. These results suggest that brain IL-1RI mediates the behavioral effects of IL-1 beta in mice.
Cremona
S
S
Institut National de la Santé et de la Recherche Médicale U394, Bordeaux, France.
Goujon
E
E
Kelley
K W
KW
Dantzer
R
R
Parnet
P
P
D000818:Animals; D000911:Antibodies, Monoclonal; D001522:Behavior, Animal / Q000502:physiology*; D001921:Brain / Q000502:physiology*; D007158:Immunologic Techniques; D007276:Injections, Intraventricular; D053590:Interleukin 1 Receptor Antagonist Protein; D008297:Male; D051379:Mice; D017472:Receptors, Interleukin-1 / Q000502:physiology*; D053573:Receptors, Interleukin-1 Type I; D053574:Receptors, Interleukin-1 Type II; D012795:Sialoglycoproteins / Q000008:administration & dosage; D012919:Social Behavior
D000911:Antibodies, Monoclonal; C507764:Il1rn protein, mouse; D053590:Interleukin 1 Receptor Antagonist Protein; D017472:Receptors, Interleukin-1; D053573:Receptors, Interleukin-1 Type I; D053574:Receptors, Interleukin-1 Type II; D012795:Sialoglycoproteins