title: Guanidine hydrochloride-induced changes of the E2 inner core of the Bacillus stearothermophilus pyruvate dehydrogenase complex.
abstract: The limited proteolysis of the Bacillus stearothermophilus pyruvate dehydrogenase complex by V8 protease yields its core structure solely composed of lipoate acetyltransferase (E2) fragments. The changes in the core with guanidine hydrochloride (GdnHCl) were biphasic: below 0.8 M (first) and above 1.0 M (second) GdnHCl. The changes in the first phase were slight but significant: decreases in ellipticity and light scattering, and an increase in E2 activity. Insignificant changes in the molecular shape and size of the core were detected on fluorescence spectroscopy, ultracentrifugation, gel filtration, and electron microscopy. On the other hand, the changes in the second phase were drastic; the core was disassembled and denatured.
authors.affiliation: Structural Biophysics Laboratory, The Institute of Physical and Chemical Research (RIKEN), Sayo-gun, Hyogo 679-5143.
authors.forename: Y
authors.initials: Y
authors.lastname: Hiromasa
authors.forename: Y
authors.initials: Y
authors.lastname: Aso
authors.forename: K
authors.initials: K
authors.lastname: Mayanagi
authors.forename: Y
authors.initials: Y
authors.lastname: Inoue
authors.forename: T
authors.initials: T
authors.lastname: Fujisawa
authors.forename: K
authors.initials: K
authors.lastname: Meno
authors.forename: T
authors.initials: T
authors.lastname: Ueki
mesh_terms: D002850:Chromatography, Gel; D001411:Geobacillus stearothermophilus / Q000201:enzymology*; D019791:Guanidine / Q000737:chemistry*; D008027:Light; D008854:Microscopy, Electron; D011487:Protein Conformation; D011768:Pyruvate Dehydrogenase Complex / Q000737:chemistry*; D012542:Scattering, Radiation; D013050:Spectrometry, Fluorescence; D014461:Ultracentrifugation
chemical_list: D011768:Pyruvate Dehydrogenase Complex; D019791:Guanidine