Sensitivity- and gradient-enhanced hetero (omega1) half-filtered TOCSY experiment for measuring long-range heteronuclear coupling constants.
An enhanced version of the X (omega1) half-filtered TOCSY experiment for measurement of long-range heteronuclear coupling constants is proposed which yields high-quality spectra with substantially increased sensitivity and resolution. The modified method features gradient-enhanced X filtering sequences, broadband homonuclear decoupling during t1, optional 1JXH scaling in the F1 domain, and gradient coherence selection in combination with the sensitivity-enhanced protocol for the TOCSY transfer. These modifications extend the applicability of the method--coupling constants can be measured accurately for natural abundance samples at low concentrations and for compounds yielding complex spectra. Computer-aided analysis of E.COSY-type multiplets is applied for the determination of heteronuclear long-range coupling constants.
Uhrín
D
D
Department of Chemistry, University of Edinburgh, United Kingdom.
Batta
G
G
Hruby
V J
VJ
Barlow
P N
PN
Kövér
K E
KE
D009682:Magnetic Resonance Spectroscopy / Q000379:methods*; D009842:Oligopeptides / Q000737:chemistry*; D012680:Sensitivity and Specificity; D014312:Trisaccharides / Q000737:chemistry*
D009842:Oligopeptides; D014312:Trisaccharides