title: In-vitro activities of paromomycin and lasalocid evaluated in combination against Cryptosporidium parvum.
abstract: Using a chemiluminescence immunoassay, paromomycin and lasalocid were shown to inhibit Cryptosporidium parvum growth in Madin-Darby canine kidney cells in a concentration-dependent manner. The median effective concentrations (EC50s) for paromomycin and lasalocid were 1184 mg/L and 0.4 mg/L, respectively. Neither drug was cytotoxic to host cells at concentrations up to five times their EC50s. Drug combination studies were conducted and the resulting data were analysed by the median-effect principle and combination index method. Statistically significant synergy was observed when combinations of paromomycin and lasalocid were used at ratios of 5000:1 and 2500:1. A possible mechanism for synergy is discussed.
authors.affiliation: Department of Pediatrics, Emory University School of Medicine, Atlanta, GA 30322, USA.
authors.forename: X
authors.initials: X
authors.lastname: You
authors.forename: R F
authors.initials: RF
authors.lastname: Schinazi
authors.forename: M J
authors.initials: MJ
authors.lastname: Arrowood
authors.forename: M
authors.initials: M
authors.lastname: Lejkowski
authors.forename: A S
authors.initials: AS
authors.lastname: Juodawlkis
authors.forename: J R
authors.initials: JR
authors.lastname: Mead
mesh_terms: D000818:Animals; D002417:Cattle; D016785:Cryptosporidium parvum / Q000187:drug effects* / Q000254:growth & development; D004285:Dogs; D004305:Dose-Response Relationship, Drug; D004359:Drug Therapy, Combination; D005434:Flow Cytometry; D007118:Immunoassay / Q000379:methods; D007668:Kidney / Q000166:cytology / Q000469:parasitology; D007832:Lasalocid / Q000494:pharmacology* / Q000633:toxicity; D008163:Luminescent Measurements; D010303:Paromomycin / Q000494:pharmacology* / Q000633:toxicity; D011419:Propidium / Q000493:pharmacokinetics
chemical_list: D011419:Propidium; D010303:Paromomycin; D007832:Lasalocid