Interferon-gamma maintains the binding and functional capacity of receptors for IL-8 on cultured human T cells.
The neutrophil chemotactic cytokine, IL-8, has been reported to also chemoattract T lymphocytes in vitro and in vivo. Previously we showed that freshly isolated T cells migrated in response to IL-8, but incubation of T cells at 37 degrees C resulted in progressively decreased levels of IL-8 binding sites on T cells in association with reduced chemotactic responses. However, this reduced binding and migration of cultured T cells in response to IL-8 can be prevented by the presence of mononuclear cells in the culture. In order to define the factor(s) responsible for the restoration of T cell binding and migration in response to IL-8, we examined the effects of various cytokines. Addition of IFN-gamma in cultured T cells maintained both the CXC chemokine receptor CXCR1 and CXCR2 binding sites for IL-8 on these cells to the level comparable to that expressed on freshly purified T cells accompanied by an almost complete restoration of their chemotactic response to IL-8. The results suggest that Th1 cytokine, IFN-gamma, produced by mononuclear cells stimulated by proinflammatory signals may play an important role in regulating IL-8 receptor expression on T cells and in sustaining the function of these cells in response to IL-8.
Tani
K
K
Laboratory of Molecular Immunoregulation, National Cancer Institute, Frederick Cancer Research and Development Center, MD 21702, USA.
Su
S B
SB
Utsunomiya
I
I
Oppenheim
J J
JJ
Wang
J M
JM
D015703:Antigens, CD / Q000032:analysis / Q000187:drug effects* / Q000378:metabolism*; D001665:Binding Sites / Q000187:drug effects / Q000276:immunology; D002478:Cells, Cultured; D002634:Chemotaxis, Leukocyte / Q000187:drug effects; D005434:Flow Cytometry / Q000706:statistics & numerical data; D006801:Humans; D007371:Interferon-gamma / Q000494:pharmacology*; D016209:Interleukin-8 / Q000378:metabolism*; D011485:Protein Binding / Q000187:drug effects / Q000276:immunology; D018123:Receptors, Interleukin / Q000032:analysis / Q000187:drug effects* / Q000378:metabolism*; D023062:Receptors, Interleukin-8A; D013601:T-Lymphocytes / Q000276:immunology / Q000378:metabolism*
D015703:Antigens, CD; D016209:Interleukin-8; D018123:Receptors, Interleukin; D023062:Receptors, Interleukin-8A; D007371:Interferon-gamma