Streptomycin inhibition of myogenic tone, K+-induced force and block of L-type calcium current in rat cerebral arteries.
1. Streptomycin has been demonstrated to inhibit mechanosensitive conductances in a wide variety of cell types, including muscle. The action of streptomycin on rat cerebral arteries that exhibit pressure-induced myogenic response was investigated. 2. Pressure-induced tone, measured using isobaric myography, in isolated pressurized cerebral arteries was reversibly and concentration-dependently inhibited by streptomycin with an IC50 of 2.6 mM. 3. Isometric K+-induced force, measured using isometric myography, is supported by voltage-gated Ca2+ entry. Streptomycin reversibly and concentration-dependently inhibited isometric force with an IC50 of 1.71 mM. 4. Voltage-gated macroscopic inward Ca2+ channel currents were recorded from freshly isolated rat basilar myocytes. These were reversibly and concentration-dependently inhibited by streptomycin with an IC50 of 1.79 and 0.47 mM when 10 and 1.8 mM CaCl2, respectively, was used as the charge carrier. 5. These data suggest that streptomycin inhibits myogenic tone and K+-induced isometric force largely by blockade of L-type, dihydropyridine-sensitive Ca2+ channels. In conclusion, streptomycin is not useful in the investigation of stretch-activated channels which may underlie the myogenic response of rat small cerebral arteries.
Miller
A L
AL
Department of Physiology, University of Bristol, University Walk, Bristol BS8 1TD, UK.
Langton
P D
PD
D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D015220:Calcium Channels / Q000502:physiology*; D020746:Calcium Channels, L-Type; D002536:Cerebral Arteries / Q000737:chemistry / Q000187:drug effects* / Q000502:physiology; D004558:Electric Stimulation; D015640:Ion Channel Gating / Q000502:physiology; D007537:Isometric Contraction / Q000187:drug effects; D008297:Male; D018485:Muscle Fibers, Skeletal / Q000737:chemistry / Q000187:drug effects / Q000502:physiology; D009124:Muscle Proteins / Q000502:physiology; D009129:Muscle Tonus / Q000187:drug effects / Q000502:physiology; D009131:Muscle, Smooth, Vascular / Q000737:chemistry / Q000187:drug effects / Q000502:physiology; D009206:Myocardium / Q000737:chemistry* / Q000166:cytology; D018408:Patch-Clamp Techniques; D011188:Potassium / Q000494:pharmacology*; D051381:Rats; D017208:Rats, Wistar; D013307:Streptomycin / Q000494:pharmacology*; D014661:Vasoconstriction / Q000187:drug effects / Q000502:physiology
D000900:Anti-Bacterial Agents; D015220:Calcium Channels; D020746:Calcium Channels, L-Type; D009124:Muscle Proteins; D011188:Potassium; D013307:Streptomycin