[The creation of genetic cryobanks and the use of the methods of developmental biology as a means for preserving rare animal species. III. The outlook, problems and limits: selection, modifications and mutations].
A review of our own and literature data about perspectives, problems and limitations in the use of cryoconservation techniques and methods of developmental biology for the conservation of genetic resources is presented. It is demonstrated that the use of these methods may result in the selection and possibly leads to modifications and mutations in germ cells and embryos and in this way may change the genetic structure of restored populations as compared with the original ones. The need to improve current techniques of cryoconservation and accompanying biotechnological procedures in order to abolish these undesirable genetic effects is emphasized.
Amstislavskiĭ
S Ia
SIa
Institute of Cytology and Genetics, Siberian Branch, Russian Academy of Sciences, Novosibirsk, Russia.
Krivokharchenko
A S
AS
Rott
N N
NN
D000818:Animals; D000835:Animals, Wild / Q000196:embryology* / Q000235:genetics; D018070:Biological Specimen Banks / Q000458:organization & administration / Q000639:trends*; D015925:Cryopreservation / Q000379:methods*; D015509:Developmental Biology / Q000379:methods*; D004624:Embryo Transfer; D004622:Embryo, Mammalian; D005260:Female; D008322:Mammals / Q000196:embryology* / Q000235:genetics; D009154:Mutation; D012641:Selection, Genetic; D013480:Superovulation
Sozdanie geneticheskikh kriobankov i ispol'zovanie metodov biologii razvitiia kak sposob sokhraneniia redkikh vidov zhivotnykh. III. Perspektivy, problemy i ogranicheniia: selektsiia, modifikatsii i mutatsii.