Adaptive response of human melanoma cells to methylglyoxal injury.
The effects of methylglyoxal on the growth of a line of human melanoma cells are investigated. Methylglyoxal inhibits cell growth in a dose-dependent manner and causes an increase in glyceraldehyde 3-phosphate dehydrogenase, and glyoxalase 1 and glyoxalase 2 specific activities. The cellular response to increasing concentrations of methylglyoxal in the culture medium is also studied by measuring L-lactate production, reduced-oxidized glutathione levels and apoptotic cell death. Methylglyoxal seems to promote a change of cell population phenotypic repertoire toward a more monomorphic phenotype. In conclusion, methylglyoxal seems to induce an enzymatic cellular response that lowers methylglyoxal levels and selects the most resistant cells.
Amicarelli
F
F
Department of Basic and Applied Biology, University of L'Aquila, Italy.
Bucciarelli
T
T
Poma
A
A
Aimola
P
P
Di Ilio
C
C
Ragnelli
A M
AM
Miranda
M
M
D017209:Apoptosis; D002455:Cell Division / Q000187:drug effects*; D003470:Culture Media; D053938:DNA Fragmentation; D005978:Glutathione / Q000378:metabolism; D019803:Glutathione Disulfide / Q000378:metabolism; D005987:Glyceraldehyde-3-Phosphate Dehydrogenases / Q000378:metabolism; D006801:Humans; D007791:Lactoylglutathione Lyase / Q000378:metabolism; D008545:Melanoma / Q000201:enzymology / Q000378:metabolism / Q000473:pathology*; D011765:Pyruvaldehyde / Q000633:toxicity*; D013869:Thiolester Hydrolases / Q000378:metabolism; D014407:Tumor Cells, Cultured; D000099301:Hydroxyacylglutathione Hydrolase
D003470:Culture Media; D005978:Glutathione; D019803:Glutathione Disulfide; D005987:Glyceraldehyde-3-Phosphate Dehydrogenases; D007791:Lactoylglutathione Lyase; D011765:Pyruvaldehyde; D013869:Thiolester Hydrolases; D000099301:Hydroxyacylglutathione Hydrolase