title: Protein phosphorylation in fast and slow chicken skeletal muscles: effect of denervation.
abstract: We have identified proteins in adult chicken skeletal muscle whose phosphorylation can be used as markers for the mature fast and slow muscle phenotype. These include phosphorylase, phosphorylase kinase, and a cyclic adenosine 3',5'-monophosphate (cAMP)-stimulated, calmodulin-inhibited 28-kDa band (markers for fast muscle), a calmodulin-stimulated 50-kDa band, and two cAMP-stimulated bands at 44 and 46 kDa (markers for slow muscle), and the relative concentrations of the regulatory subunits of cAMP-dependent protein kinase (RI and RII). After denervation the pattern of phosphorylation in fast muscle changed to resemble that of slow muscle: phosphorylation of the fast phenotype markers decreased; the slow phenotype markers, barely detectable in normal fast muscle, appeared as significant phosphoproteins; and the concentration of RII increased with no change in RI. This is consistent with denervation-induced changes observed using other phenotypic markers and indicates the potential for using these phosphoprotein markers in studies of muscle development and pathophysiology.
authors.affiliation: The Neuroscience Group, Faculty of Medicine and Health Sciences, The University of Newcastle, Callaghan, NSW, Australia.
authors.forename: X
authors.initials: X
authors.lastname: Wang
authors.forename: J A
authors.initials: JA
authors.lastname: Rostas
mesh_terms: D000818:Animals; D015415:Biomarkers / Q000032:analysis; D002118:Calcium / Q000378:metabolism; D002645:Chickens; D000242:Cyclic AMP / Q000378:metabolism; D003600:Cytosol / Q000378:metabolism; D008566:Membranes / Q000378:metabolism; D009121:Muscle Denervation; D018656:Muscle Fibers, Fast-Twitch / Q000378:metabolism* / Q000648:ultrastructure; D018657:Muscle Fibers, Slow-Twitch / Q000378:metabolism* / Q000648:ultrastructure; D009124:Muscle Proteins / Q000378:metabolism*; D018482:Muscle, Skeletal / Q000294:innervation / Q000378:metabolism* / Q000648:ultrastructure; D010641:Phenotype; D010750:Phosphoproteins / Q000378:metabolism; D010766:Phosphorylation
chemical_list: D015415:Biomarkers; D009124:Muscle Proteins; D010750:Phosphoproteins; D000242:Cyclic AMP; D002118:Calcium