title: Bordetella pertussis filamentous hemagglutinin enhances the immunogenicity of liposome-delivered antigen administered intranasally.
abstract: In an attempt to increase the immunogenicity of mucosally delivered antigens, we incorporated the Bordetella pertussis filamentous hemagglutinin (FHA) adhesin into liposomes containing the glutathione S-transferase of Schistosoma mansoni (Sm28GST) as a model antigen. Outbred mice immunized twice intranasally with liposomes containing a constant suboptimal dose of Sm28GST and increasing doses of FHA produced anti-Sm28GST antibodies in a FHA dose-dependent manner. The addition of 3 microg of FHA to the liposomes induced more than 10-fold-higher anti-Sm28GST antibody titers, compared to those induced by liposomes without FHA. The presence of FHA did not alter the nature of the humoral immune response, and the sera contained anti-Sm28GST immunoglobulin G1 (IgG1), IgG2a, and IgG2b. However, anti-Sm28GST IgA was only detected when at least 3 microg of FHA was added to the preparation. These results show a promising potential for FHA to enhance the immunogenicity of mucosally administered antigens incorporated into liposomes.
authors.affiliation: INSERM U167, Institut Pasteur de Lille, France. odile.poulain@pasteur-lille.fr
authors.forename: O
authors.initials: O
authors.lastname: Poulain-Godefroy
authors.forename: N
authors.initials: N
authors.lastname: Mielcarek
authors.forename: N
authors.initials: N
authors.lastname: Ivanoff
authors.forename: F
authors.initials: F
authors.lastname: Remoué
authors.forename: A M
authors.initials: AM
authors.lastname: Schacht
authors.forename: N
authors.initials: N
authors.lastname: Phillips
authors.forename: C
authors.initials: C
authors.lastname: Locht
authors.forename: A
authors.initials: A
authors.lastname: Capron
authors.forename: G
authors.initials: G
authors.lastname: Riveau
mesh_terms: D018829:Adhesins, Bacterial / Q000008:administration & dosage* / Q000276:immunology; D000276:Adjuvants, Immunologic / Q000008:administration & dosage*; D000281:Administration, Intranasal; D000818:Animals; D000909:Antibodies, Helminth / Q000097:blood; D005982:Glutathione Transferase / Q000276:immunology*; D006388:Hemagglutinins / Q000008:administration & dosage* / Q000276:immunology; D007074:Immunoglobulin G / Q000097:blood / Q000145:classification; D008081:Liposomes; D051379:Mice; D012550:Schistosoma mansoni / Q000276:immunology*; D010566:Virulence Factors, Bordetella
chemical_list: D018829:Adhesins, Bacterial; D000276:Adjuvants, Immunologic; D000909:Antibodies, Helminth; D006388:Hemagglutinins; D007074:Immunoglobulin G; D008081:Liposomes; D010566:Virulence Factors, Bordetella; C065273:filamentous hemagglutinin adhesin, Bordetella pertussis; D005982:Glutathione Transferase