An experimental study of the effect of aprotinin on intestinal adhesion formation.
BACKGROUND
Depression of fibrinolysis is known to be a major mechanism for postoperative adhesion formation. Because aprotinin inhibits fibrinolysis it may lead to an increase in adhesion formation whereas its anti-inflammatory effects may lead to a decrease in adhesion formation. Our aim is to clarify conflicting results in previous literature.


METHODS
Basal levels of intestinal hydroxyproline (OHP) content and local fibrinolytic activity (LFA) were determined using naive groups. In the experiment groups, adhesions were created by scraping and creating a transient ischemia of a segment of terminal ileum. Group I and II rats were injected subcutaneous (s.c.) normal saline (NS) for 3 days and single dose intraperitoneal (i.p.) NS, respectively. Group III and IV rats were injected s.c. aprotinin for 3 days and single dose i.p. aprotinin, respectively. Group V rats were injected intramuscular methylprednisolone (MP) for 3 days. LFA and OHP levels were determined on the second and fifth postoperative days. The severity of adhesion formation was graded on the fifth day.


RESULTS
Aprotinin decreased both the severity of adhesions and OHP levels whereas MP decreased only the severity of adhesions. There was an early depression of LFA at the second day in both NS and MP groups increasing to basal levels at the fifth day. OHP levels showed significant correlation with adhesion severity.


CONCLUSIONS
Results showed that aprotinin decreased intra-abdominal adhesion formation probably by preventing early depression of LFA.
Ozoğul
Y
Y
Department of General Surgery, Tokat State Hospital, Turkey.
Baykal
A
A
Onat
D
D
Renda
N
N
Sayek
I
I
D000818:Animals; D007611:Aprotinin / Q000494:pharmacology*; D005342:Fibrinolysis / Q000187:drug effects*; D006490:Hemostatics / Q000494:pharmacology*; D006909:Hydroxyproline / Q000032:analysis; D007410:Intestinal Diseases / Q000503:physiopathology / Q000517:prevention & control*; D007421:Intestine, Small / Q000737:chemistry; D051381:Rats; D017208:Rats, Wistar; D000267:Tissue Adhesions / Q000503:physiopathology / Q000517:prevention & control
D006490:Hemostatics; D007611:Aprotinin; D006909:Hydroxyproline