Escherichia coli defects caused by DnaK and DnaJ proteins overproduction.
Escherichia coli defects caused by the overproduction of DnaK and DnaJ proteins are presented. Induced overproduction of DnaK and DnaJ was determined by SDS-PAGE electrophoresis and immunoblots analysis. Elevated levels of both proteins are not deleterious for such cellular functions as growth, survival and cell morphology. However, instability of pJW14 plasmid (derivative of pACYC184) was observed as the result of dnaKdnaJ operon overexpression.
Lobacz
B
B
Department of Bacterial Genetics, University of Warsaw, Poland.
Wolska
K I
KI
D015169:Colony Count, Microbial; D004591:Electrophoresis, Polyacrylamide Gel; D004926:Escherichia coli / Q000235:genetics* / Q000254:growth & development* / Q000378:metabolism; D029968:Escherichia coli Proteins; D050956:HSP40 Heat-Shock Proteins; D018840:HSP70 Heat-Shock Proteins / Q000096:biosynthesis* / Q000235:genetics; D006360:Heat-Shock Proteins / Q000096:biosynthesis* / Q000235:genetics; D015151:Immunoblotting; D007544:Isopropyl Thiogalactoside / Q000494:pharmacology; D009876:Operon; D010957:Plasmids / Q000235:genetics; D013053:Spectrophotometry
C494635:DnaJ protein, E coli; D029968:Escherichia coli Proteins; D050956:HSP40 Heat-Shock Proteins; D018840:HSP70 Heat-Shock Proteins; D006360:Heat-Shock Proteins; D007544:Isopropyl Thiogalactoside; C063842:dnaK protein, E coli