title: A novel sulfonic-acid analogue of ceramide is the major extractable lipid of the gram-negative marine bacterium Cyclobacterium marinus WH.
abstract: The extractable lipids of the gram-negative, sea-water bacterium Cyclobacterium marinus strain WH contain about 94% of polar components which consist of two phospholipids, phosphatidylethanolamine (29% of the total lipids) and phosphatidylcholine (7%), and two phosphorus-free lipids. One of the latter has been shown to be a novel sulfonic-acid analogue of ceramide, 2-D-(2'-D-hydroxy-13'-methyltetradecanoyl) amino-3-D-hydroxy-15-methylhexadec-4 (E)-en-1-sulfonic acid (48%), and other is a lipodipeptide, N-[3-d-(13'-methyltetradecanoyloxy)-15-methylhexadecanoyl] glycyl-L-serine (11%), which has so far been found only in a Flavobacterium sp. strain. The dominant fatty acid residues of the phospholipids are iso-15:0, n-16:0, 16:1 and 18:1, the acyl residues linked to the sn-1 carbon of the glycerol moiety being somewhat more saturated as compared with those located at the sn-2 position. A new procedure for determination of the absolute configuration of 2- and 3-hydroxy fatty acids is briefly described.
authors.affiliation: Research Centre "Hydrobios" of Ministry of Health, ul. Kosmonavtov 18, korp. 2, Moscow 129301, Russian Federation.
authors.forename: S G
authors.initials: SG
authors.lastname: Batrakov
authors.forename: D I
authors.initials: DI
authors.lastname: Nikitin
authors.forename: V I
authors.initials: VI
authors.lastname: Sheichenko
authors.forename: A O
authors.initials: AO
authors.lastname: Ruzhitsky
mesh_terms: D002518:Ceramides / Q000737:chemistry*; D005227:Fatty Acids / Q000032:analysis; D006090:Gram-Negative Bacteria / Q000737:chemistry*; D008055:Lipids / Q000737:chemistry*; D009682:Magnetic Resonance Spectroscopy; D013058:Mass Spectrometry; D008968:Molecular Conformation; D015394:Molecular Structure; D010455:Peptides / Q000737:chemistry; D010713:Phosphatidylcholines / Q000032:analysis; D010714:Phosphatidylethanolamines / Q000032:analysis; D010743:Phospholipids / Q000032:analysis; D013451:Sulfonic Acids / Q000737:chemistry*
chemical_list: D002518:Ceramides; D005227:Fatty Acids; D008055:Lipids; D010455:Peptides; D010713:Phosphatidylcholines; D010714:Phosphatidylethanolamines; D010743:Phospholipids; D013451:Sulfonic Acids