title: Measuring protein-protein interactions.
abstract: The binding of one protein to another provokes a variety of biophysical changes that can then be used as a measure of the binding reaction. Optical spectroscopy, particularly fluorescence, is the most flexible technique, but surface plasmon resonance biosensors, microcalorimetry and mass spectroscopy have recently shown significant development.
authors.affiliation: Department of Biochemistry and Genetics, Medical School, University of Newcastle upon Tyne, UK. J.H.Lakey@ncl.ac.uk
authors.forename: J H
authors.initials: JH
authors.lastname: Lakey
authors.forename: E M
authors.initials: EM
authors.lastname: Raggett
mesh_terms: D001665:Binding Sites; D002151:Calorimetry; D002942:Circular Dichroism; D005453:Fluorescence; D013058:Mass Spectrometry; D011485:Protein Binding; D011506:Proteins / Q000737:chemistry / Q000378:metabolism*
chemical_list: D011506:Proteins