title: Resident human hepatic lymphocytes are phenotypically different from circulating lymphocytes.
abstract: OBJECTIVE
  Murine and human studies have documented the existence of subpopulations of lymphocytes in particular tissues that differ phenotypically and functionally from those in peripheral blood and may mature locally. Since little is known about lymphocyte subpopulations in the normal human liver, we have analysed the surface phenotypes of lymphocytes isolated from liver specimens taken from 15 donors at the time of liver transplantation, and compared these with those of peripheral blood lymphocytes.
  
  
  METHODS
  Hepatic lymphocytes were prepared by mechanical dissociation and enzymatic digestion of liver tissue. The cells were stained with a panel of monoclonal antibodies (CD3, CD4, CD8, CD19, CD56, gammadeltaTCR, alphabetaTCR, CD8alpha-chain, CD8alphabeta dimer), and analysed by flow cytometry. In situ characterisation of hepatic lymphocytes was by haematoxylin and eosin staining of fixed liver sections and by immunohistochemical staining for common leukocyte antigen and CD3.
  
  
  RESULTS
  Significant numbers of hepatic T lymphocytes were localised to the portal tracts and parenchyma of normal liver specimens. Flow cytometry revealed that the CD4/CD8 ratio (1:3.5) was consistently reversed compared with that in peripheral blood (2:1). Other lymphocyte populations identified include double positive CD3+CD4+CD8+ cells which accounted for a mean of 5.5% (range 3-11.6%) of hepatic CD3+ cells compared with 1.3% in blood (range 0.7-3.6%; p < 0.007), and double negative CD3+ CD4-8- cells (14.5%; range 2.7-29% compared with 5.0%; range 2.1-10.8%, p < 0.02). Over 15% (range 6.8-34%) of all hepatic CD3+ cells expressed a gammadeltaTCR compared to 2.7% (range 0.9-4.7%) of CD3+ peripheral blood lymphocytes (p < 0.004) and almost 50% of these coexpressed CD8. The CD8 alpha-chain was expressed without the beta-chain (CD8alpha+beta-) by 15.4% (range 4-29.1%) of hepatic T cells, but this phenotype was undetectable among peripheral blood lymphocytes (p < 0.009). Cells expressing both the T cell marker CD3 and the natural killer cell marker CD56 constituted 31.6% (range 14-54%) of all hepatic CD3+ lymphocytes but were rarely present amongst peripheral blood lymphocytes (0-6%; p < 0.0001).
  
  
  CONCLUSIONS
  These data are the first to describe and quantify unconventional T lymphocyte subpopulations in the normal adult human liver which may have specialised functions in regional immune responses and which may differentiate locally. These findings have important implications for our understanding of hepatic immunoregulation and the pathogenic mechanisms involved in viral and immune-mediated liver disease and allograft rejection.
authors.affiliation: Education & Research Centre and Liver Unit, St. Vincent's Hospital, and University College Dublin, Ireland.
authors.forename: S
authors.initials: S
authors.lastname: Norris
authors.forename: C
authors.initials: C
authors.lastname: Collins
authors.forename: D G
authors.initials: DG
authors.lastname: Doherty
authors.forename: F
authors.initials: F
authors.lastname: Smith
authors.forename: G
authors.initials: G
authors.lastname: McEntee
authors.forename: O
authors.initials: O
authors.lastname: Traynor
authors.forename: N
authors.initials: N
authors.lastname: Nolan
authors.forename: J
authors.initials: J
authors.lastname: Hegarty
authors.forename: C
authors.initials: C
authors.lastname: O'Farrelly
mesh_terms: D000293:Adolescent; D000328:Adult; D000818:Animals; D000911:Antibodies, Monoclonal; D015703:Antigens, CD / Q000032:analysis; D002648:Child; D005260:Female; D006801:Humans; D016130:Immunophenotyping; D008099:Liver / Q000166:cytology / Q000276:immunology*; D016131:Lymphocyte Subsets / Q000166:cytology / Q000276:immunology; D008214:Lymphocytes / Q000166:cytology* / Q000276:immunology*; D008297:Male; D051379:Mice; D008875:Middle Aged; D016692:Receptors, Antigen, T-Cell, gamma-delta / Q000032:analysis; D012016:Reference Values; D013601:T-Lymphocytes / Q000166:cytology / Q000276:immunology
chemical_list: D000911:Antibodies, Monoclonal; D015703:Antigens, CD; D016692:Receptors, Antigen, T-Cell, gamma-delta