title: A Xenopus cysteine string protein with a cysteine residue in the J domain.
abstract: A cDNA clone encoding a Xenopus cysteine string protein (Xcsp) was isolated and sequenced. The deduced primary sequence of Xcsp is very similar to other vertebrate csps with the exception of a cysteine residue that lies outside of the cysteine-string domain. This cysteine residue replaces a serine that is highly conserved among vertebrate csps, and thus may be of functional importance. Xcsp mRNA appears as a 4.6 kb species on Northern analysis, and immunoblot of Xenopus brain membranes reveals a single, 35 kDa Xcsp that can be deacylated, like other csps.
authors.affiliation: Department of Molecular and Medical Pharmacology, UCLA School of Medicine 90095, USA.
authors.forename: A
authors.initials: A
authors.lastname: Mastrogiacomo
authors.forename: H I
authors.initials: HI
authors.lastname: Kornblum
authors.forename: J A
authors.initials: JA
authors.lastname: Umbach
authors.forename: C B
authors.initials: CB
authors.lastname: Gundersen
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001923:Brain Chemistry; D003001:Cloning, Molecular; D003545:Cysteine / Q000235:genetics*; D018076:DNA, Complementary / Q000235:genetics; D050956:HSP40 Heat-Shock Proteins; D008565:Membrane Proteins; D008969:Molecular Sequence Data; D009419:Nerve Tissue Proteins / Q000737:chemistry / Q000235:genetics*; D012333:RNA, Messenger / Q000032:analysis; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D014982:Xenopus laevis / Q000235:genetics*
chemical_list: D018076:DNA, Complementary; D050956:HSP40 Heat-Shock Proteins; D008565:Membrane Proteins; D009419:Nerve Tissue Proteins; D012333:RNA, Messenger; C088460:cysteine string protein; D003545:Cysteine