Cloning and sequencing of cDNA encoding the LS-12 antifreeze protein in the longhorn sculpin, Myoxocephalus octodecimspinosis.
cDNA coding for an antifreeze protein (LS-12) in the longhorn sculpin, Myoxocephalus octodecimspinosis, was prepared from liver mRNA using reverse transcriptase-polymerase chain reaction coupled with 3' and 5' RACE procedures. This cDNA contains 609 base pairs, including a 384-bp open reading frame which codes for a 128-residue LS-12 precursor protein. The predicted amino acid sequence of the mature LS-12 corresponds exactly to the amino acid sequence obtained from Edman degradation [G. Deng, D.W. Andrews, R.A. Laursen, FEBS Lett., 402, 1997, pp. 17-20]. The 20 residues preceding mature LS-12 are predicted to be a signal sequence, which is presumably cleaved off before the mature, 108-residue protein is secreted into the circulatory system. This is the first report of a cDNA sequence from M. octodecimspinosis.
Zhao
Z
Z
Department of Biochemistry, Boston University School of Medicine, MA 02118, USA.
Deng
G
G
Lui
Q
Q
Laursen
R A
RA
D000595:Amino Acid Sequence; D000818:Animals; D021302:Antifreeze Proteins, Type IV; D001483:Base Sequence; D003001:Cloning, Molecular; D029941:Fish Proteins; D008099:Liver / Q000737:chemistry; D008969:Molecular Sequence Data; D011498:Protein Precursors / Q000737:chemistry; D021382:Protein Sorting Signals / Q000737:chemistry; D011506:Proteins / Q000737:chemistry*; D017422:Sequence Analysis, DNA
D021302:Antifreeze Proteins, Type IV; D029941:Fish Proteins; C103901:LS-12 antifreeze protein, Myoxocephalus octodecemspinosus; D011498:Protein Precursors; D021382:Protein Sorting Signals; D011506:Proteins