Effect of Ginkor Fort on hypoxia-induced neutrophil adherence to human saphenous vein endothelium.
This study was performed to evaluate the effects of Ginkor Fort, a venotropic drug composed of Ginkgo biloba extract, troxerutine, and heptaminol, on neutrophil adherence to the endothelium of saphenous veins. When saphenous veins were incubated 2 h in hypoxic conditions, they showed a five- to sixfold increase in neutrophil adherence to the endothelium. Ginkor Fort at 0.3 mg/ml was able to inhibit this increase by 69%. These results were confirmed by observations in scanning electron microscopy. Ginkor Fort also inhibited the subsequent activation of these neutrophils, as evidenced by the inhibition of superoxide anion release. The biochemical mechanism of this inhibition of neutrophil adherence was studied on endothelial cells in culture. We observed that Ginkor Fort was able to inhibit the different steps of the activation of endothelial cells by hypoxia: the activation of phospholipase A2 and the decrease in adenosine triphosphate (ATP) content. By preventing the first step of the activation cascade, the decrease in ATP content, Ginkor Fort blocks the subsequent increase in neutrophil adherence as well as neutrophil activation. The biochemical mechanism evidenced in this work might explain the beneficial effect of this drug in the treatment of patients with chronic venous insufficiency.
Arnould
T
T
Laboratoire de Biochimie et Biologie Cellulaire, Facultés Universitaires Notre-Dame de la Paix, Namur, Belgium.
Michiels
C
C
Janssens
D
D
Berna
N
N
Remacle
J
J
D002448:Cell Adhesion / Q000187:drug effects*; D015687:Cell Hypoxia; D002478:Cells, Cultured; D004730:Endothelium, Vascular / Q000166:cytology / Q000187:drug effects* / Q000648:ultrastructure; D005419:Flavonoids / Q000494:pharmacology*; D020441:Ginkgo biloba; D006490:Hemostatics / Q000494:pharmacology*; D006801:Humans; D066298:In Vitro Techniques; D008855:Microscopy, Electron, Scanning; D009504:Neutrophils / Q000166:cytology / Q000187:drug effects* / Q000648:ultrastructure; D010936:Plant Extracts; D012501:Saphenous Vein / Q000166:cytology / Q000187:drug effects* / Q000648:ultrastructure; D000096983:Ginkgo Extract
D005419:Flavonoids; D006490:Hemostatics; D010936:Plant Extracts; C063170:Ginkgo biloba extract; D000096983:Ginkgo Extract