Interaction of sulfhydryl reagents with K+ transport in adrenal chromaffin granules.
In the present study the functional role of SH groups in the Ca(2+)-independent K(+)-selective channel activity in the membrane of bovine adrenal gland chromaffin granules has been studied. Ionic channel activity has been estimated using 86Rb+, a K+ analogue, flux measurements. The inhibition of chromaffin granules K+ channel by SH modifying agents, such as N-ethylmaleimide, mersalyl and phenylarsenoxide, is described.
Szewczyk
A
A
Department of Molecular and Cell Neurobiology, Nencki Institute of Experimental Biology, Warsaw, Poland. adam@nencki.gov.pl.
Lobanov
N A
NA
Nowotny
M
M
Nałecz
M J
MJ
D000313:Adrenal Medulla / Q000166:cytology / Q000378:metabolism; D000818:Animals; D001692:Biological Transport / Q000502:physiology; D002417:Cattle; D019439:Chromaffin Cells / Q000737:chemistry / Q000187:drug effects* / Q000378:metabolism*; D011188:Potassium / Q000378:metabolism*; D015221:Potassium Channels / Q000502:physiology; D013439:Sulfhydryl Reagents / Q000494:pharmacology*
D015221:Potassium Channels; D013439:Sulfhydryl Reagents; D011188:Potassium