title: Alternative splicing and genomic organization of the L5-67 gene of Aplysia californica.
abstract: The L5-67 gene was first identified on the basis of its high expression level in the LUQ neurons, a group of four giant cells located in the left upper quadrant of the abdominal ganglion of Aplysia californica. Its mRNA and peptides were later shown to be present in these cells, as well as in about 100 other smaller neurons in the CNS. L5-67 propeptide and/or mature peptides are also present in peripheral organs, particularly in the kidney, which is the target of most LUQ processes. Using RT-PCR, we show the presence of an alternatively spliced L5-67 transcript arising from the exclusion of the fourth exon from the mature mRNA. This alternative splicing event occurs specifically in the kidney, although we could not identify the cells in which it takes place. Translation of this transcript generates a 52 amino acid (aa) propeptide in which the first N-terminal 45 aa are identical to the original L5-67 propeptide. The last seven C-terminal aa are unrelated to the previously characterized L5-67 peptides due to a change in the open reading frame.
authors.affiliation: University of Montreal, Department of Biochemistry, Quebec, Canada.
authors.forename: A
authors.initials: A
authors.lastname: Angers
authors.forename: L
authors.initials: L
authors.lastname: DesGroseillers
mesh_terms: D017398:Alternative Splicing; D000595:Amino Acid Sequence; D000818:Animals; D001048:Aplysia / Q000235:genetics* / Q000378:metabolism; D001483:Base Sequence; D003001:Cloning, Molecular; D005091:Exons; D017952:Ganglia, Invertebrate / Q000378:metabolism*; D015698:Genomic Library; D017403:In Situ Hybridization; D007668:Kidney / Q000378:metabolism; D008969:Molecular Sequence Data; D009420:Nervous System / Q000378:metabolism; D009474:Neurons / Q000378:metabolism*; D009479:Neuropeptides / Q000096:biosynthesis / Q000737:chemistry / Q000235:genetics*; D009928:Organ Specificity; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000032:analysis / Q000096:biosynthesis; D014158:Transcription, Genetic
chemical_list: C111666:L5-67 protein, Aplysia californica; D009479:Neuropeptides; D012333:RNA, Messenger