title: A sex-linked Ace gene, not linked to insensitive acetylcholinesterase-mediated insecticide resistance in Culex pipiens.
abstract: An acetylcholinesterase (AChE) gene, Ace.x, showing 93% identity of deduced amino acid sequence to Anopheles stephensi Ace has been cloned from a Culex pipiens strain homozygous for insensitive AChE (iAChE) mediated insecticide resistance. DNA sequence of genomic DNA clones identified exons 2-5. RFLP of six clones indicated four possible alleles. Linkage analysis located Ace.x to chromosome I, less than 0.8 centimorgans from the sex locus, whereas the locus conferring resistance was 2.0 centimorgans from plum-eye on chromosome II. Ace.1 coding for AChE1, which is associated with resistance, is therefore autosomal. We propose that Ace.x is the recently postulated Ace.2 coding for the biochemically distinct AChE2, which is not associated with resistance.
authors.affiliation: School of Biological Sciences, Queen Mary and Westfield College, University of London, UK. c.a.malcolm@qmw.ac.uk
authors.forename: C A
authors.initials: CA
authors.lastname: Malcolm
authors.forename: D
authors.initials: D
authors.lastname: Bourguet
authors.forename: A
authors.initials: A
authors.lastname: Ascolillo
authors.forename: S J
authors.initials: SJ
authors.lastname: Rooker
authors.forename: C F
authors.initials: CF
authors.lastname: Garvey
authors.forename: L M
authors.initials: LM
authors.lastname: Hall
authors.forename: N
authors.initials: N
authors.lastname: Pasteur
authors.forename: M
authors.initials: M
authors.lastname: Raymond
mesh_terms: D000110:Acetylcholinesterase / Q000235:genetics*; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003465:Culex / Q000201:enzymology* / Q000235:genetics; D018076:DNA, Complementary; D005260:Female; D006801:Humans; D007305:Insecticide Resistance / Q000235:genetics; D008297:Male; D008969:Molecular Sequence Data; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D012737:Sex Factors
chemical_list: D018076:DNA, Complementary; D000110:Acetylcholinesterase