title: Alterations in the GyrA subunit of DNA gyrase and the ParC subunit of DNA topoisomerase IV associated with quinolone resistance in Enterococcus faecalis.
abstract: The gyrA and parC genes of 31 clinical isolates of Enterococcus faecalis, including fluoroquinolone-resistant isolates, were partially sequenced and analyzed for target alterations. Topoisomerase IV may be a primary target in E. faecalis, but high-level fluoroquinolone resistance was associated with simultaneous alterations in both GyrA and ParC.
authors.affiliation: Department of Urology, Gifu University School of Medicine, Gifu City, Japan.
authors.forename: E
authors.initials: E
authors.lastname: Kanematsu
authors.forename: T
authors.initials: T
authors.lastname: Deguchi
authors.forename: M
authors.initials: M
authors.lastname: Yasuda
authors.forename: T
authors.initials: T
authors.lastname: Kawamura
authors.forename: Y
authors.initials: Y
authors.lastname: Nishino
authors.forename: Y
authors.initials: Y
authors.lastname: Kawada
mesh_terms: D042462:4-Quinolones; D000595:Amino Acid Sequence; D000890:Anti-Infective Agents / Q000494:pharmacology*; D027081:DNA Gyrase; D027101:DNA Topoisomerase IV; D004250:DNA Topoisomerases, Type II / Q000187:drug effects / Q000235:genetics*; D004352:Drug Resistance, Microbial / Q000235:genetics; D013293:Enterococcus faecalis / Q000187:drug effects* / Q000201:enzymology / Q000235:genetics*; D005798:Genes, Bacterial / Q000235:genetics*; D008969:Molecular Sequence Data; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid
chemical_list: D042462:4-Quinolones; D000890:Anti-Infective Agents; D027101:DNA Topoisomerase IV; D027081:DNA Gyrase; D004250:DNA Topoisomerases, Type II