Application of nitrite reductase from Alcaligenes faecalis S-6 for nitrite measurement.
The enzymatic reaction of nitrite reductase (NIR) from Alcaligenes faecalis S-6 was applied to the measurement of nitrite. NIR was immobilized on the surface of a gold electrode using filter paper and a dialysis membrane, and used as a working electrode in a three-electrode system. Amperometric methods were applied using NIR and the electron mediator 1-methoxy PMS (1-methoxy-5-methylphenazinium methylsulfate). The decrease in cathodic current showed a correlation to nitrite concentration over the range 0-1 mg/l. Measurements using a batch-flow type system gave a lower detection limit of 0.01 mg/l. This is sufficient for the detection of nitrite in natural waters.
Sasaki
S
S
Research Center for Advanced Science and Technology, University of Tokyo, Japan.
Karube
I
I
Hirota
N
N
Arikawa
Y
Y
Nishiyama
M
M
Kukimoto
M
M
Horinouchi
S
S
Beppu
T
T
D000421:Alcaligenes / Q000201:enzymology*; D015374:Biosensing Techniques; D009572:Nitrite Reductases; D009573:Nitrites / Q000032:analysis*; D012680:Sensitivity and Specificity; D014867:Water / Q000032:analysis
D009573:Nitrites; D014867:Water; D009572:Nitrite Reductases