title: Protein oxidation: examination of potential lipid-independent mechanisms for protein carbonyl formation.
abstract: Previous data indicated that diquat-mediated protein oxidation (protein carbonyl formation) occurs through multiple pathways, one of which is lipid dependent, and the other, lipid independent. Studies reported here investigated potential mechanisms of the lipid-independent pathway in greater detail, using bovine serum albumin as the target protein. One hypothesized mechanism of protein carbonyl formation involved diquat-dependent production of H2O2, which would then react with site-specifically bound ferrous iron as proposed by Stadtman and colleagues. This hypothesis was supported by the inhibitory effect of catalase on diquat-mediated protein carbonyl formation. However, exogenous H2O2 alone did not induce protein carbonyl formation. Hydroxyl radical-generating reactions may result from the H2O2-catalyzed oxidation of ferrous iron, which normally is bound to protein in the ferric state. Therefore, the possible reduction of site-specifically bound Fe3+ to Fe2+ by the diquat cation radical (which could then react with H2O2) was also investigated. The combination of H2O2 and an iron reductant, ascorbate, however, also failed to induce significant protein carbonyl formation. In a phospholipid-containing system, an ADP:Fe2+ complex induced both lipid peroxidation and protein carbonyl formation; both indices were largely inhibitable by antioxidants. There was no substantial ADP:Fe(2+)-dependent protein carbonyl formation in the absence of phospholipid under otherwise identical conditions. Based on the lipid requirement and antioxidant sensitivity, these data suggest that ADP:Fe(2+)-dependent protein carbonyl formation occurs through reaction of BSA with aldehydic lipid peroxidation products. The precise mechanism of diquat-mediated protein carbonyl formation remains unclear, but it appears not to be a function of H2O2 generation or diquat cation radical-dependent reduction of bound Fe3+.
authors.affiliation: Pharmacia & Upjohn, Inc., Kalamazoo, MI 49001, USA.
authors.forename: D P
authors.initials: DP
authors.lastname: Blakeman
authors.forename: T P
authors.initials: TP
authors.lastname: Ryan
authors.forename: R A
authors.initials: RA
authors.lastname: Jolly
authors.forename: T W
authors.initials: TW
authors.lastname: Petry
mesh_terms: D000244:Adenosine Diphosphate / Q000031:analogs & derivatives / Q000378:metabolism; D000818:Animals; D000975:Antioxidants / Q000633:toxicity; D002839:Chromans / Q000633:toxicity*; D004178:Diquat / Q000633:toxicity*; D006540:Herbicides / Q000633:toxicity; D006861:Hydrogen Peroxide / Q000378:metabolism; D015227:Lipid Peroxidation / Q000187:drug effects; D008297:Male; D008862:Microsomes, Liver / Q000187:drug effects / Q000201:enzymology; D009251:NADPH-Ferrihemoprotein Reductase / Q000378:metabolism; D010084:Oxidation-Reduction / Q000187:drug effects; D010743:Phospholipids / Q000502:physiology*; D010879:Piperazines / Q000633:toxicity*; D011506:Proteins / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley; D017392:Thiobarbituric Acid Reactive Substances / Q000378:metabolism
chemical_list: D000975:Antioxidants; D002839:Chromans; D006540:Herbicides; D010743:Phospholipids; D010879:Piperazines; D011506:Proteins; D017392:Thiobarbituric Acid Reactive Substances; C029936:adenosine diphosphate-ferric chelate; C066382:U 78517F; D000244:Adenosine Diphosphate; D004178:Diquat; D006861:Hydrogen Peroxide; D009251:NADPH-Ferrihemoprotein Reductase