title: Enhanced metastasis of B16 melanoma cells by unexpected elevated expression of the metastasis-associated TI-241 (LRF-1-, Jun-Fos-related) gene treated with antisense oligonucleotide.
abstract: B16-F10 is a B16 mouse melanoma subline that preferentially metastasizes to the lung following intravenous injection. Previously we isolated TI-241 (LRF-1 homologue related to Jun-Fos) gene that was expressed higher in the high metastatic clone B16-F10 than the low metastatic clone F1. Transfection of TI-241 into F1 converted it into a high-metastatic cell. We studied the effect of antisense oligonucleotide designed to reduce the expression of TI-241 in B16-F10 cells, and observed an unexpected increase in the TI-241 level. The increase in the expression was maximal at 30 h, then it decreased during further culture with or without TI-241 antisense oligonucleotide. This increased TI-241 expression by antisense oligonucleotide was also observed in B16-F1 cells whereas sense oligonucleotide did not affect the expression. B16-F10 cells cultured with TI-241 antisense oligonucleotide showed enhanced experimental metastatic potential to the mouse lungs compared with untreated B16-F10 and B16-F10 cultured with TI-241 sense oligonucleotide.
authors.affiliation: First Department of Surgery, Institute of Molecular and Cellular Biosciences, The University of Tokyo, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Ishiguro
authors.forename: M
authors.initials: M
authors.lastname: Naito
authors.forename: K
authors.initials: K
authors.lastname: Hanaoka
authors.forename: H
authors.initials: H
authors.lastname: Nagawa
authors.forename: T
authors.initials: T
authors.lastname: Muto
authors.forename: T
authors.initials: T
authors.lastname: Tsuruo
mesh_terms: D051700:Activating Transcription Factor 3; D000818:Animals; D004268:DNA-Binding Proteins / Q000235:genetics*; D016350:Leucine Zippers; D008175:Lung Neoplasms / Q000556:secondary; D008546:Melanoma, Experimental / Q000235:genetics* / Q000473:pathology; D051379:Mice; D009362:Neoplasm Metastasis; D016376:Oligonucleotides, Antisense / Q000494:pharmacology; D014407:Tumor Cells, Cultured
chemical_list: D051700:Activating Transcription Factor 3; D004268:DNA-Binding Proteins; D016376:Oligonucleotides, Antisense; C068616:liver regeneration factor 1