title: Nucleotide and amino acid sequences of the metallo-beta-lactamase, ImiS, from Aeromonas veronii bv. sobria.
abstract: The Aeromonas veronii bv. sobria metallo-beta-lactamase gene, imiS, was cloned. The imiS open reading frame extends for 762 bp and encodes a protein of 254 amino acids with a secreted modified protein of 227 amino acids and a predicted pI of 8.1. To confirm the predicted sequence, purified ImiS was digested and the resulting peptides were identified, yielding an identical sequence for ImiS, with 98% identity to CphA. Both possessed the putative active-site sequence Asn-Tyr-His-Thr-Asp at positions 88 to 92, which is unique to the Aeromonas metallo-beta-lactamases.
authors.affiliation: Bristol Centre for Antimicrobial Research and Evaluation, Department of Microbiology and Pathology, Medical School, University of Bristol, United Kingdom. T.R.WALSH@BRISTOL.AC.UK
authors.forename: T R
authors.initials: TR
authors.lastname: Walsh
authors.forename: W A
authors.initials: WA
authors.lastname: Neville
authors.forename: M H
authors.initials: MH
authors.lastname: Haran
authors.forename: D
authors.initials: D
authors.lastname: Tolson
authors.forename: D J
authors.initials: DJ
authors.lastname: Payne
authors.forename: J H
authors.initials: JH
authors.lastname: Bateson
authors.forename: A P
authors.initials: AP
authors.lastname: MacGowan
authors.forename: P M
authors.initials: PM
authors.lastname: Bennett
mesh_terms: D000333:Aeromonas / Q000201:enzymology / Q000235:genetics*; D000595:Amino Acid Sequence; D001426:Bacterial Proteins; D001483:Base Sequence; D005798:Genes, Bacterial / Q000235:genetics*; D008969:Molecular Sequence Data; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid; D001618:beta-Lactamases / Q000235:genetics*
chemical_list: D001426:Bacterial Proteins; C111082:ImiS protein, Aeromonas veronii; D001618:beta-Lactamases