title: Human monocyte-endothelial cell interaction induces platelet-derived growth factor expression.
abstract: OBJECTIVE
  The purpose of this study was to investigate whether the synthesis of platelet-derived growth factor (PDGF), a major mitogen and chemoattractant for vascular smooth muscle cells, was induced by the direct cell-to-cell interaction between human monocytes and umbilical vein endothelial cells (ECs).
  
  
  METHODS
  PDGF protein and mRNA expression were determined by cellular ELISA, immunohistochemical and Northern blot analyses.
  
  
  RESULTS
  Coculture of monocytes and ECs secreted a large amount of PDGF into the supernatant, whereas culture of ECs or monocytes alone induced low levels of PDGF production. In Northern blot analysis, substantial amounts of PDGF-A and -B mRNA were induced by coculture of monocytes with ECs. Immunohistochemistry revealed that PDGF-B chain protein was detectable in both ECs and monocytes. PDGF production by ECs induced by conditioned medium of the coculture was significantly inhibited by Abs against interleukin-1 beta (IL-1 beta) and tumor necrosis factor- alpha (TNF alpha).
  
  
  CONCLUSIONS
  These results indicate that the direct cell-to-cell interaction between human monocytes and ECs induces PDGF synthesis in both types of cells, suggesting that PDGF produced locally by monocyte-EC adhesive interaction plays an important role in the pathogenesis of atherosclerosis by promoting the migration and accumulation of vascular smooth muscle cells.
authors.affiliation: Department of Cardiology, Jichi Medical School, Tochigi, Japan.
authors.forename: H
authors.initials: H
authors.lastname: Funayama
authors.forename: U
authors.initials: U
authors.lastname: Ikeda
authors.forename: M
authors.initials: M
authors.lastname: Takahashi
authors.forename: Y
authors.initials: Y
authors.lastname: Sakata
authors.forename: S
authors.initials: S
authors.lastname: Kitagawa
authors.forename: Y
authors.initials: Y
authors.lastname: Takahashi
authors.forename: J
authors.initials: J
authors.lastname: Masuyama
authors.forename: Y
authors.initials: Y
authors.lastname: Furukawa
authors.forename: Y
authors.initials: Y
authors.lastname: Miura
authors.forename: S
authors.initials: S
authors.lastname: Kano
authors.forename: M
authors.initials: M
authors.lastname: Matsuda
authors.forename: K
authors.initials: K
authors.lastname: Shimada
mesh_terms: D000911:Antibodies, Monoclonal / Q000494:pharmacology; D001161:Arteriosclerosis / Q000209:etiology; D015152:Blotting, Northern; D002448:Cell Adhesion / Q000502:physiology; D002450:Cell Communication / Q000502:physiology*; D018920:Coculture Techniques; D004730:Endothelium, Vascular / Q000502:physiology*; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D007150:Immunohistochemistry; D007375:Interleukin-1 / Q000276:immunology / Q000502:physiology; D009000:Monocytes / Q000502:physiology*; D010982:Platelet-Derived Growth Factor / Q000032:analysis / Q000096:biosynthesis* / Q000235:genetics; D012313:RNA / Q000032:analysis; D014409:Tumor Necrosis Factor-alpha / Q000276:immunology / Q000502:physiology; D014471:Umbilical Veins
chemical_list: D000911:Antibodies, Monoclonal; D007375:Interleukin-1; D010982:Platelet-Derived Growth Factor; D014409:Tumor Necrosis Factor-alpha; D012313:RNA