Simultaneous measurement of venlafaxine and its major metabolite, oxydesmethylvenlafaxine, in human plasma by high-performance liquid chromatography with coulometric detection and utilisation of solid-phase extraction.
Venlafaxine, oxydesmethylvenlafaxine and an internal standard (paroxetine) were extracted from plasma by a solid-phase extraction technique. Chromatography was performed using isocratic reversed-phase high-performance liquid chromatography (HPLC) with coulometric endpoint detection. The standard curves were linear over the range 0-200 ng/ml for both venlafaxine and oxydesmethylvenlafaxine in plasma. The mean inter- and intra-assay coefficients of variation over the range of the standard curves were less than 10%. The absolute recovery averaged 74% for venlafaxine and 67% for oxydesmethylvenlafaxine. The sensitivity was 0.5 ng for both the analytes. Plasma profiles of the analytes following oral administration of venlafaxine, are presented.
Clement
E M
EM
University of Oxford, Department of Psychiatry, Warneford Hospital, UK.
Odontiadis
J
J
Franklin
M
M
D000284:Administration, Oral; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D003511:Cyclohexanols / Q000097:blood* / Q000493:pharmacokinetics / Q000592:standards; D000069468:Desvenlafaxine Succinate; D006801:Humans; D017374:Paroxetine / Q000032:analysis / Q000592:standards; D012015:Reference Standards; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D000069470:Venlafaxine Hydrochloride
D003511:Cyclohexanols; D017374:Paroxetine; D000069470:Venlafaxine Hydrochloride; D000069468:Desvenlafaxine Succinate