title: Predictors of luminal narrowing by neointima after angioplasty in atherosclerotic rabbits.
abstract: OBJECTIVE
  The present study was designed to identify the predictors of cross-sectional area narrowing by neointima (%CSAN-N) after balloon angioplasty (BA) in the cholesterol fed rabbit model.
  
  
  METHODS
  Angiographic, histomorphometric, and immunohistochemical data were analyzed from 91 femoral arteries of New Zealand white rabbits. Focal atherosclerosis was induced by air desiccation of the endothelium followed by a 2% cholesterol diet for 28 days. The rabbits received heparin (150 U/kg) at the time of BA (2.5 mm; three, 60-second, 10-atm inflations). Arteries were perfusion-fixed and excised 7 (n = 16), 14 (n = 11), 21 (n = 9), or 28 (n = 20) days after BA. Non-angioplastied arteries were de-endothelialized (cholesterol-fed [n = 12] or normal diet [n = 8]), non-injured but cholesterol-fed (n = 7), or normal (n = 8).
  
  
  RESULTS
  Univariate regression across all groups showed that the absolute area of the lumen by histomorphometry (LA) correlated significantly with the area bounded by the external elastic lamina (EEL) (vessel size), but no correlation was found with the absolute area of neointima or media, the percentage disruption of the internal elastic lamina (IEL), or the percentage of neointima and media occupied by foam cells. However, %CSAN-N correlated significantly with the area bounded by the EEL, significantly with the absolute neointimal area, and negatively with the absolute LA (p < 0.0001). Significant correlations were also found between %CSAN-N and the % IEL disrupted, the area of neointima and media occupied by RAM-11 + foam cells, and the loss of alpha-actin positivity in the media (p < 0.0001).
  
  
  CONCLUSIONS
  These studies show that neointimal formation contributes significantly to luminal narrowing 1 month after angioplasty in this model, that the degree of vascular injury and the extent of foam cell accumulation in the neointima and media are significant independent predictors of neointimal formation, and that the area of the neointima, and the percent narrowing by neointima, are important predictors of remodeling itself (EEL area). These predictors were not identifiable when the analysis was focused on the determinants of absolute luminal area alone.
authors.affiliation: Department of Anatomy and Cell Biology, Hebrew University-Hadassah Medical School, Jerusalem, Israel. gertz@yam-suff.cc.huji.ac.il
authors.forename: S D
authors.initials: SD
authors.lastname: Gertz
authors.forename: W L
authors.initials: WL
authors.lastname: Barry
authors.forename: L W
authors.initials: LW
authors.lastname: Gimple
authors.forename: S
authors.initials: S
authors.lastname: Banai
authors.forename: L S
authors.initials: LS
authors.lastname: Perez
authors.forename: C A
authors.initials: CA
authors.lastname: McNamara
authors.forename: E R
authors.initials: ER
authors.lastname: Powers
authors.forename: M
authors.initials: M
authors.lastname: Ragosta
authors.forename: G K
authors.initials: GK
authors.lastname: Owens
authors.forename: W C
authors.initials: WC
authors.lastname: Roberts
authors.forename: I J
authors.initials: IJ
authors.lastname: Sarembock
mesh_terms: D000704:Analysis of Variance; D000800:Angioplasty, Balloon / Q000009:adverse effects; D000818:Animals; D001161:Arteriosclerosis / Q000209:etiology / Q000473:pathology / Q000628:therapy*; D002452:Cell Count; D002791:Cholesterol, Dietary / Q000009:adverse effects; D005263:Femoral Artery / Q000293:injuries* / Q000473:pathology; D005487:Foam Cells / Q000473:pathology; D011184:Postoperative Period; D011379:Prognosis; D011817:Rabbits; D012008:Recurrence; D012044:Regression Analysis; D017539:Tunica Intima / Q000473:pathology
chemical_list: D002791:Cholesterol, Dietary