Evaluation of histological structure and its effect on the distribution of alpha1-adrenoceptors in human benign prostatic hyperplasia.
OBJECTIVE
To evaluate the histological structure of benign prostatic hyperplasia (BPH) and its relationship with the density of alpha1-adrenoceptors in smooth muscle.


METHODS
Specimens from hyperplastic tissues obtained from 14 patients with BPH were evaluated for the density of alpha1-adrenoceptors in smooth muscle using autoradiography, Mallory-Azan staining and computer-assisted image analyses. The binding of [3H] tamsulosin (a selective alpha1-blocker) and the ratio of smooth muscle area was calculated, and the density of alpha1-adrenoceptors per area of smooth muscle determined by dividing the degree of binding by the ratio of smooth muscle to total area.


RESULTS
There was a significant difference between the ratio of smooth muscle area in the hyperplastic acinar nodule and the surrounding stroma (P < 0.01). The density of alpha1-adrenoceptor per smooth muscle area was significantly higher in the hyperplastic acinar nodule than in the surrounding stroma (P < 0.05). There was no correlation between prostatic weight and the ratio of smooth muscle area or the density of alpha1-adrenoceptors in each region.


CONCLUSIONS
The distribution of alpha1-adrenoceptors on smooth muscle differed with histological structure; both the histological conformation and the difference in the distribution of alpha1-adrenoceptors could affect urethral obstruction in BPH.
Kurimoto
S
S
Department of Urology, Faculty of Medicine, The University of Tokyo, Japan.
Moriyama
N
N
Hamada
K
K
Kawabe
K
K
D001345:Autoradiography / Q000379:methods; D006801:Humans; D007090:Image Interpretation, Computer-Assisted; D008297:Male; D009130:Muscle, Smooth / Q000737:chemistry*; D009929:Organ Size; D011467:Prostate / Q000737:chemistry* / Q000473:pathology; D011470:Prostatic Hyperplasia / Q000473:pathology*; D018340:Receptors, Adrenergic, alpha-1 / Q000032:analysis*; D013194:Staining and Labeling
D018340:Receptors, Adrenergic, alpha-1