Optical monitoring of living brain tissue.
Optical methods can usefully augment classical techniques such as electrical recording for investigating cellular and network physiology. Here, recent developments in optical methods--including confocal and nonlinear fluorescence imaging, semiconductor array imaging, and improved fluorescent indicators of ion concentration and transmembrane electrical potential--are briefly reviewed, and examples are offered that may suggest potential applications to experimental studies in alcoholism research.
Hosokawa
T
T
Hokkaido University School of Medicine, Sapporo, Japan.
Ohta
M
M
Koyama
A
A
Saito
K
K
Fine
A
A
D000437:Alcoholism / Q000473:pathology / Q000503:physiopathology; D000818:Animals; D001921:Brain / Q000187:drug effects* / Q000473:pathology; D001931:Brain Mapping / Q000295:instrumentation*; D046508:Culture Techniques; D000431:Ethanol / Q000494:pharmacology*; D006624:Hippocampus / Q000473:pathology / Q000503:physiopathology; D007091:Image Processing, Computer-Assisted / Q000295:instrumentation*; D008564:Membrane Potentials / Q000187:drug effects / Q000502:physiology; D018613:Microscopy, Confocal / Q000295:instrumentation*; D008856:Microscopy, Fluorescence / Q000295:instrumentation*
D000431:Ethanol