A simple whole-blood test for detecting antibodies to human immunodeficiency virus.
We developed an immunochromatographic whole-blood test (WBT) which detects antibodies to human immunodeficiency virus types 1 and 2 (HIV-1 and HIV-2) from fingerstick blood. The sensitivity and specificity of the WBT were 99.41% (1,018 confirmed positive patients) and 99.89% (941 uninfected patients), respectively (enzyme immunoassay [EIA] on serum or plasma as a reference). WBT performance was comparable to those of licensed EIAs and Western blotting, using 18 HIV-2 sera, 23 HIV-1 seroconversion panels, and a low-titer performance panel (in lieu of whole blood).
Schramm
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W
Saliva Diagnostic Systems, Inc., Vancouver, Washington 98682-2444, USA.
Wade
S E
SE
Barriga Angulo
G
G
Castillo Torres
P
P
Burgess-Cassler
A
A
D015658:HIV Infections / Q000097:blood / Q000175:diagnosis* / Q000276:immunology; D015497:HIV-1 / Q000276:immunology*; D015498:HIV-2 / Q000276:immunology*; D006801:Humans; D012698:Serologic Tests / Q000379:methods*