title: Exoenzyme S from P. aeruginosa ADP ribosylates rab4 and inhibits transferrin recycling in SLO-permeabilized reticulocytes.
abstract: ADP-ribosylation of rab proteins by exoenzyme S (Exo S) of P. aeruginosa was studied using reticulocytes. 14-3-3 protein, the eukaryotic cofactor that is obligatory for Exo S activity, was found in association with reticulocyte endocytic vesicles and exosomes, vesicles previously shown to be enriched with rab4. Incubation of purified endocytic vesicles with Exo S triggered rab4 ADP-ribosylation. Transferrin recycling in SLO-permeabilized reticulocytes was highly impaired when Exo S was added to the cells, suggesting that ADP-ribosylation affected rab4 function. Moreover, in vitro ADP-ribosylation of different rab proteins was studied using the cofactor activity extracted from reticulocytes.
authors.affiliation: UMR 5539 CNRS, Université Montpellier II, France.
authors.forename: P
authors.initials: P
authors.lastname: Bette-Bobillo
authors.forename: P
authors.initials: P
authors.lastname: Giro
authors.forename: J
authors.initials: J
authors.lastname: Sainte-Marie
authors.forename: M
authors.initials: M
authors.lastname: Vidal
mesh_terms: D048948:14-3-3 Proteins; D036002:ADP Ribose Transferases; D000246:Adenosine Diphosphate Ribose / Q000378:metabolism*; D000595:Amino Acid Sequence; D000818:Animals; D001426:Bacterial Proteins; D001427:Bacterial Toxins; D002463:Cell Membrane Permeability; D003067:Coenzymes / Q000378:metabolism; D016384:Consensus Sequence; D019204:GTP-Binding Proteins / Q000235:genetics / Q000378:metabolism; D066298:In Vitro Techniques; D008969:Molecular Sequence Data; D011065:Poly(ADP-ribose) Polymerases / Q000378:metabolism*; D011506:Proteins / Q000378:metabolism; D011550:Pseudomonas aeruginosa / Q000201:enzymology*; D051381:Rats; D012156:Reticulocytes / Q000378:metabolism*; D013301:Streptolysins; D014168:Transferrin / Q000378:metabolism; D014446:Tyrosine 3-Monooxygenase; D020695:rab4 GTP-Binding Proteins
chemical_list: D048948:14-3-3 Proteins; D001426:Bacterial Proteins; D001427:Bacterial Toxins; D003067:Coenzymes; D011506:Proteins; D013301:Streptolysins; D014168:Transferrin; C025964:streptolysin O; D000246:Adenosine Diphosphate Ribose; D014446:Tyrosine 3-Monooxygenase; D036002:ADP Ribose Transferases; D011065:Poly(ADP-ribose) Polymerases; C444820:exoenzyme S; D019204:GTP-Binding Proteins; D020695:rab4 GTP-Binding Proteins