Interaction of rat lin-10 with brain-enriched F-actin-binding protein, neurabin-II/spinophilin.
We have recently isolated a rat homologue of the Caenorrhabditis elegans lin-10 product. Although rat lin-10 is expressed in the cytosol and membrane fractions of various tissues, it is distributed only in the membrane fraction in brain where it is enriched in the synaptic plasma membrane and postsynaptic density fractions. We have isolated here a rat lin-10-interacting protein from rat brain and identified it to be neurabin-II/spinophilin, which has recently been isolated as a protein interacting with protein phosphatase I and F-actin. Neurabin-II/spinophilin is ubiquitously expressed but enriched in brain, especially in the synaptic plasma membrane and postsynaptic density fractions. We discuss the physiological significance of the interaction of rat lin-10 with neurabin-II/spinophilin.
Ide
N
N
Takai Biotimer Project, ERATO, Japan Science and Technology Corporation, Kobe.
Hata
Y
Y
Hirao
K
K
Irie
M
M
Deguchi
M
M
Yao
I
I
Satoh
A
A
Wada
M
M
Takahashi
K
K
Nakanishi
H
H
Takai
Y
Y
D000199:Actins / Q000378:metabolism*; D000818:Animals; D015374:Biosensing Techniques; D001921:Brain / Q000378:metabolism*; D019556:COS Cells; D029742:Caenorhabditis elegans Proteins; D002352:Carrier Proteins; D002460:Cell Line; D002478:Cells, Cultured; D003001:Cloning, Molecular; D015801:Helminth Proteins / Q000378:metabolism*; D006624:Hippocampus / Q000166:cytology / Q000378:metabolism; D006801:Humans; D007668:Kidney; D008565:Membrane Proteins; D008840:Microfilament Proteins / Q000378:metabolism*; D009419:Nerve Tissue Proteins / Q000378:metabolism*; D009474:Neurons / Q000378:metabolism; D011506:Proteins; D051381:Rats; D017386:Sequence Homology, Amino Acid; D000099273:Neurabins
D000199:Actins; D029742:Caenorhabditis elegans Proteins; D002352:Carrier Proteins; D015801:Helminth Proteins; D008565:Membrane Proteins; D008840:Microfilament Proteins; D009419:Nerve Tissue Proteins; D011506:Proteins; D000099273:Neurabins; C034037:F-actin-binding proteins; C064105:lin-10 protein, C elegans