Estimate of the frequency of human immunodeficiency virus type 1 protease inhibitor resistance within unselected virus populations in vitro.
The frequency of drug-resistant human immunodeficiency virus type 1 (HIV-1) variants in virus populations not previously exposed to drug was determined in vitro by using HIV-1RF and the protease inhibitor SC-55389A. Two variants with single mutations responsible for drug resistance (V82A and N88S) were quantifiably isolated after only one round of replication, yielding a crude frequency estimate of at least 1 SC-55389A-resistant variant per 3.5 x 10(5) wild-type infectious units.
Tucker
S P
SP
G. D. Searle, Searle Research and Development, St. Louis, Missouri 63198, USA. simon.t@biota.com.au
Stiebel
T R
TR
Potts
K E
KE
Smidt
M L
ML
Bryant
M L
ML
D004352:Drug Resistance, Microbial / Q000235:genetics; D017320:HIV Protease Inhibitors / Q000494:pharmacology*; D015497:HIV-1 / Q000187:drug effects* / Q000235:genetics; D006801:Humans; D017354:Point Mutation; D014508:Urea / Q000031:analogs & derivatives* / Q000494:pharmacology
D017320:HIV Protease Inhibitors; C105835:SC 55389A; D014508:Urea