ZFX and ZFY loci in water buffalo (Bubalus bubalis): potential for sex identification.
In the present study a small region of ZFX and ZFY loci in buffalo have been amplified by polymerase chain reaction (PCR). Restriction fragment length polymorphism (RFLP) was also uncovered that can distinguish between male and female buffalo DNA. The study also found a second copy of the ZFX loci (ZFXR) present in both male and female. Sequence analysis showed that ZFXR has a single base deletion that results in a redundant putative protein.
Pande
A
A
Embryo Biotechnology Laboratory, National Institute of Immunology, Aruna Asaf Ali Marg, New Delhi, India.
Totey
S M
SM
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002020:Buffaloes / Q000235:genetics*; D003001:Cloning, Molecular; D004268:DNA-Binding Proteins / Q000235:genetics*; D005260:Female; D051741:Kruppel-Like Transcription Factors; D008297:Male; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction / Q000379:methods; D012150:Polymorphism, Restriction Fragment Length; D017422:Sequence Analysis, DNA; D012689:Sequence Homology, Nucleic Acid; D012732:Sex Determination Analysis / Q000379:methods*; D014157:Transcription Factors; D016335:Zinc Fingers / Q000235:genetics
D004268:DNA-Binding Proteins; D051741:Kruppel-Like Transcription Factors; D014157:Transcription Factors; C062076:zinc finger protein, X-linked