title: Multiple actions of nitric oxide on voltage-dependent Ca2+ channels in mouse cerebral cortical neurons.
abstract: We investigated the effects of nitric oxide (NO) on voltage-dependent Ca2+ channels (VDCCs) by examining [45Ca2+]influx into mouse cerebral cortical neurons. S-nitroso-N-acetylpenicillamine (SNAP) induced a dose-dependent increase in [45Ca2+]influx, which was completely abolished by hemoglobin, tetrodotoxin and dibucaine. The NO-induced [45Ca2+influx was significantly inhibited by verapamil and omega-agatoxin VIA (omega-AGX), whereas omega-conotoxin GVIA (omega-CTX) had no effects on the NO-induced [45Ca2+]influx. KCl (30 mM) stimulated [45Ca2+]influx, and verapamil, omega-CTX and omega-AGX reduced the KCl-induced [45Ca2+]influx by about 40, 26 and 34%, respectively, indicating that the neurons used here possess L-, N- and P-typed VDCCs. SNAP itself reduced KCl-induced [45Ca2+]influx by about 28.5%. In the presence of both KCl and SNAP, omega-CTX showed no effects on the influx, while verapamil and omega-AGX significantly inhibited the influx and the concomitant presence of verapamil and omega-AGX completely abolished the influx. These results indicate that NO induces [45Ca2+] influx via the opening of L- and P-typed VDCCs subsequent to neuronal membrane depolarization and that NO itself inhibited the function of N-typed VDCC in the cerebral cortical neurons.
authors.affiliation: Department of Pharmacology, Kawasaki Medical School, Kurashiki, Japan.
authors.forename: S
authors.initials: S
authors.lastname: Ohkuma
authors.forename: M
authors.initials: M
authors.lastname: Katsura
authors.forename: Y
authors.initials: Y
authors.lastname: Hibino
authors.forename: J
authors.initials: J
authors.lastname: Xu
authors.forename: K
authors.initials: K
authors.lastname: Shirotani
authors.forename: K
authors.initials: K
authors.lastname: Kuriyama
mesh_terms: D000818:Animals; D002118:Calcium / Q000378:metabolism*; D002121:Calcium Channel Blockers / Q000494:pharmacology; D015220:Calcium Channels / Q000187:drug effects / Q000378:metabolism*; D002132:Calcium Radioisotopes; D002478:Cells, Cultured; D002540:Cerebral Cortex / Q000166:cytology / Q000378:metabolism*; D005333:Fetus; D015640:Ion Channel Gating / Q000187:drug effects*; D051379:Mice; D009474:Neurons / Q000187:drug effects / Q000378:metabolism*; D009569:Nitric Oxide / Q000494:pharmacology*; D010396:Penicillamine / Q000031:analogs & derivatives / Q000494:pharmacology; D011189:Potassium Chloride / Q000494:pharmacology; D026423:S-Nitroso-N-Acetylpenicillamine
chemical_list: D002121:Calcium Channel Blockers; D015220:Calcium Channels; D002132:Calcium Radioisotopes; D009569:Nitric Oxide; D011189:Potassium Chloride; D026423:S-Nitroso-N-Acetylpenicillamine; D010396:Penicillamine; D002118:Calcium