title: Characterization of the brome mosaic virus movement protein expressed in E. coli.
abstract: The biochemical and functional properties of the movement protein (MP) of brome mosaic virus (BMV) were investigated. Expression and purification of the BMV MP from Escherichia coli resulted in a pure and soluble protein preparation. Sucrose gradient centrifugation revealed that BMV MP forms oligomers consisting of two or more copies but no higher order multimers even when different ionic strengths and pHs were applied. Nitro-cellulose filter binding and gel retardation studies showed that in vitro the BMV MP preferentially bound to ss nucleic acids (RNA and DNA); the affinity to ssRNA was lower compared to BMV coat protein. The binding to ss nucleic acid was cooperative and not sequence specific and the hypothetical binding site was calculated to be around three to six nucleotides per MP monomer. The nucleic acid binding properties of the BMV MP are discussed in relation to the recent finding that this protein is also able to form tubular structures in infected protoplasts.
authors.affiliation: Department of Virology, Wageningen Agricultural University, Binnenhaven, The Netherlands.
authors.forename: K A
authors.initials: KA
authors.lastname: Jansen
authors.forename: C J
authors.initials: CJ
authors.lastname: Wolfs
authors.forename: H
authors.initials: H
authors.lastname: Lohuis
authors.forename: R W
authors.initials: RW
authors.lastname: Goldbach
authors.forename: B J
authors.initials: BJ
authors.lastname: Verduin
mesh_terms: D017795:Bromovirus / Q000472:pathogenicity*; D004279:DNA, Viral / Q000032:analysis; D004268:DNA-Binding Proteins / Q000737:chemistry; D004926:Escherichia coli / Q000737:chemistry; D053830:Plant Viral Movement Proteins; D012367:RNA, Viral / Q000032:analysis; D016601:RNA-Binding Proteins / Q000737:chemistry; D014764:Viral Proteins / Q000737:chemistry / Q000302:isolation & purification / Q000502:physiology*
chemical_list: D004279:DNA, Viral; D004268:DNA-Binding Proteins; D053830:Plant Viral Movement Proteins; D012367:RNA, Viral; D016601:RNA-Binding Proteins; D014764:Viral Proteins