Dot immunobinding assay for detection of mite antigens in house-dust samples.
A new test was developed specifically to detect mite antigens in house-dust. It uses a nitrocellulose dipstick spotted with specific antimite antibodies that act as a capture matrix; the same antibodies act as a detecting reagent when conjugated with colloidal dye particles. Aclotest is a 1-step assay, where a spotted dipstick is placed in a tube containing the detecting reagent and the house-dust sample. No instrumentation or previous extraction procedure of the sample is required, and the test response is visible as a colored spot, after 1 h incubation. The sensitivity and specificity of the new test were compared with those of Acarex and Der p1/Der f1 ELISA tests.
Mistrello
G
G
Research Department, Laboratorio Farmaceutico Lofarma, Milan, Italy.
Gentili
M
M
Roncarolo
D
D
Antoniotti
P
P
Ottoboni
F
F
Falagiani
P
P
D000818:Animals; D000918:Antibody Specificity; D000941:Antigens / Q000032:analysis*; D004391:Dust / Q000032:analysis*; D006798:Housing; D006801:Humans; D008925:Mites
D000941:Antigens; D004391:Dust