Protein phosphorylation in fast and slow chicken skeletal muscles: effect of denervation.
We have identified proteins in adult chicken skeletal muscle whose phosphorylation can be used as markers for the mature fast and slow muscle phenotype. These include phosphorylase, phosphorylase kinase, and a cyclic adenosine 3',5'-monophosphate (cAMP)-stimulated, calmodulin-inhibited 28-kDa band (markers for fast muscle), a calmodulin-stimulated 50-kDa band, and two cAMP-stimulated bands at 44 and 46 kDa (markers for slow muscle), and the relative concentrations of the regulatory subunits of cAMP-dependent protein kinase (RI and RII). After denervation the pattern of phosphorylation in fast muscle changed to resemble that of slow muscle: phosphorylation of the fast phenotype markers decreased; the slow phenotype markers, barely detectable in normal fast muscle, appeared as significant phosphoproteins; and the concentration of RII increased with no change in RI. This is consistent with denervation-induced changes observed using other phenotypic markers and indicates the potential for using these phosphoprotein markers in studies of muscle development and pathophysiology.
Wang
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The Neuroscience Group, Faculty of Medicine and Health Sciences, The University of Newcastle, Callaghan, NSW, Australia.
Rostas
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JA
D000818:Animals; D015415:Biomarkers / Q000032:analysis; D002118:Calcium / Q000378:metabolism; D002645:Chickens; D000242:Cyclic AMP / Q000378:metabolism; D003600:Cytosol / Q000378:metabolism; D008566:Membranes / Q000378:metabolism; D009121:Muscle Denervation; D018656:Muscle Fibers, Fast-Twitch / Q000378:metabolism* / Q000648:ultrastructure; D018657:Muscle Fibers, Slow-Twitch / Q000378:metabolism* / Q000648:ultrastructure; D009124:Muscle Proteins / Q000378:metabolism*; D018482:Muscle, Skeletal / Q000294:innervation / Q000378:metabolism* / Q000648:ultrastructure; D010641:Phenotype; D010750:Phosphoproteins / Q000378:metabolism; D010766:Phosphorylation
D015415:Biomarkers; D009124:Muscle Proteins; D010750:Phosphoproteins; D000242:Cyclic AMP; D002118:Calcium