Identification of the CC chemokines TARC and macrophage inflammatory protein-1 beta as novel functional ligands for the CCR8 receptor.
Chemokines are key molecules in directing leukocyte migration toward sites of inflammation. We have previously cloned a putative CC chemokine receptor gene, TER1, whose expression is restricted to lymphoid tissues and cell lines. Recently, this receptor has been shown to signal in response to the human CC chemokine I-309 and thus it has been renamed CCR8 according to the current nomenclature. In the present study, we report the identification of the CC chemokines thymus and activation-regulated cytokine (TARC) and macrophage inflammatory protein-1 beta (MIP-1 beta) as CCR8 ligands, as they induce chemotaxis in CCR8 Jurkat stable transfectants. Furthermore, we have generated a polyclonal antiserum that is able to recognize the CCR8 molecule in transfectant lysates. The pattern of CCR8 mRNA expression and the functional effects exerted by its ligand suggest that the triggering of this receptor may regulate multiple functions including activation, migration and proliferation of lymphoid cells.
Bernardini
G
G
Regina Elena Cancer Institute, Department of Experimental Medicine and Pathology, University of Rome, La Sapienza, Italy.
Hedrick
J
J
Sozzani
S
S
Luini
W
W
Spinetti
G
G
Weiss
M
M
Menon
S
S
Zlotnik
A
A
Mantovani
A
A
Santoni
A
A
Napolitano
M
M
D002460:Cell Line; D054402:Chemokine CCL1; D054414:Chemokine CCL17; D054407:Chemokine CCL4; D018925:Chemokines / Q000378:metabolism* / Q000502:physiology*; D019742:Chemokines, CC; D002630:Chemotactic Factors / Q000494:pharmacology; D002634:Chemotaxis, Leukocyte / Q000187:drug effects; D006388:Hemagglutinins / Q000235:genetics; D006801:Humans; D007106:Immune Sera / Q000096:biosynthesis; D019169:Jurkat Cells; D007668:Kidney; D008024:Ligands; D019402:Macrophage Inflammatory Proteins / Q000378:metabolism* / Q000502:physiology*; D054401:Receptors, CCR8; D019707:Receptors, Chemokine / Q000235:genetics / Q000276:immunology / Q000378:metabolism*; D014162:Transfection
C065971:CCL1 protein, human; C106230:CCL17 protein, human; C103330:CCR8 protein, human; D054402:Chemokine CCL1; D054414:Chemokine CCL17; D054407:Chemokine CCL4; D018925:Chemokines; D019742:Chemokines, CC; D002630:Chemotactic Factors; D006388:Hemagglutinins; D007106:Immune Sera; D008024:Ligands; D019402:Macrophage Inflammatory Proteins; D054401:Receptors, CCR8; D019707:Receptors, Chemokine