De novo peptide sequencing in an ion trap mass spectrometer with 18O labeling.
De novo peptide sequencing in an ion trap mass spectrometer coupled on-line with a capillary HPLC using 18O labeling provides a viable alternative to the method using the combination of nanospray, 18O labeling and a quadrupole/time-of-flight mass spectrometer. Seven to sixteen amino acid residues can be sequenced from the liquid chromatography/randem mass spectrometry (LC/MS/MS) spectra. This approach combines the benefit of capillary LC and the high sensitivity of the ion trap operated in the MS/MS mode. The wide availability of the LCQ mass spectrometer makes this approach readily adaptable to the biological mass spectrometry community.
Qin
J
J
Laboratory of Biophysical Chemistry, National Heart, Lung, and Blood Institute, NIH, Bethesda, MD 20892, USA.
Herring
C J
CJ
Zhang
X
X
D000595:Amino Acid Sequence; D000818:Animals; D006801:Humans; D007202:Indicators and Reagents; D013058:Mass Spectrometry / Q000295:instrumentation / Q000379:methods*; D008969:Molecular Sequence Data; D018995:Myosin Heavy Chains / Q000032:analysis; D010103:Oxygen Isotopes; D010455:Peptides / Q000032:analysis*; D011817:Rabbits; D017421:Sequence Analysis / Q000295:instrumentation*
D007202:Indicators and Reagents; D010103:Oxygen Isotopes; D010455:Peptides; D018995:Myosin Heavy Chains