Characterization, mapping and partial cDNA sequence of the 57-kD intracellular Ki-1 antigen.
A novel antigen was identified by the cross-reactivity of the anti-CD30 antibody Ki-1. This 57-kD intracellular Ki-1 antigen (Ki-1/57) is induced upon activation of leukocytes and is transported to the nuclear compartment. We describe the partial cloning and sequencing of the Ki-1/57 cDNA from a lambda gt 11-cDNA library derived from the Hodgkin-analogous cell line L540. New monoclonal antibodies were produced against the recombinant Ki-1/57 protein fragment which were used to confirm that the Ki-1/57 antigen is associated with kinase activity and is expressed in a variety of tumor cell lines and in activated but not resting leukocytes. The Ki-1/57 gene was mapped to the bands 9q22.3-31 of human chromosome 9. This is an area which appears to be associated with secondary chromosomal aberrations in acute myeloid leukemias.
Kobarg
J
J
Bristol-Myers Squibb, Pharmaceutical Research Institute, Princeton, N.J. 08543-4000, USA. Jorg_Kobarg@ccmail.bms.com
Schnittger
S
S
Fonatsch
C
C
Lemke
H
H
Bowen
M A
MA
Buck
F
F
Hansen
H P
HP
D000595:Amino Acid Sequence; D000818:Animals; D016367:Baculoviridae; D001483:Base Sequence; D002460:Cell Line; D002871:Chromosome Banding; D002899:Chromosomes, Human, Pair 9; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000737:chemistry; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D017730:Ki-1 Antigen / Q000235:genetics*; D051379:Mice; D008807:Mice, Inbred BALB C; D008969:Molecular Sequence Data; D008970:Molecular Weight; D015183:Restriction Mapping; D018411:Spodoptera
D018076:DNA, Complementary; D017730:Ki-1 Antigen