title: Determination of N-methylsuccinimide and 2-hydroxy-N-methylsuccinimide in human urine and plasma.
abstract: A method for determination of N-methylsuccinimide (MSI) and 2-hydroxy-N-methylsuccinimide (2-HMSI) in human urine and of MSI in human plasma was developed. MSI and 2-HMSI are metabolites of the widely used organic solvent N-methyl-2-pyrrolidone (NMP). MSI and 2-HMSI were purified from urine and plasma by C8 solid-phase extraction and analysed by gas chromatography-mass spectrometry in the negative-ion chemical ionisation mode. The intra-day precisions in urine were 2-6% for MSI (50 and 400 ng/ml) and 3-5% for 2-HMSI (1000 and 8000 ng/ml). For MSI in plasma it was 2% (60 and 1200 ng/ml). The between-day precisions in urine were 3-4% for MSI (100 and 1000 ng/ml) and 2-4% for 2-HMSI (10,000 and 18,000 ng/ml) and 3-4% for MSI in plasma (100 and 900 ng/ml). The recoveries from urine were 109-117% for MSI (50 and 400 ng/ml) and 81-89% for 2-HMSI (1000 and 8000 ng/ml). The recovery of MSI from plasma was 91-101% (50 and 500 ng/ml). The detection limits for MSI were 3 ng/ml in urine and 1 ng/ml in plasma and that of 2-HMSI in urine was 200 ng/ml. The method is applicable for analysis of urine and plasma samples from workers exposed to NMP.
authors.affiliation: Department of Occupational and Environmental Medicine, Institute of Laboratory Medicine, University Hospital, Lund, Sweden.
authors.forename: B A
authors.initials: BA
authors.lastname: Jönsson
authors.forename: B
authors.initials: B
authors.lastname: Akesson
mesh_terms: D004355:Drug Stability; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods; D006801:Humans; D016273:Occupational Exposure; D011760:Pyrrolidinones / Q000378:metabolism; D011786:Quality Control; D012680:Sensitivity and Specificity; D012997:Solvents; D013388:Succinimides / Q000097:blood* / Q000652:urine*; D013723:Teratogens / Q000378:metabolism
chemical_list: C107901:2-hydroxy-N-methylsuccinimide; C110990:N-methylsuccinimide; D011760:Pyrrolidinones; D012997:Solvents; D013388:Succinimides; D013723:Teratogens; C038678:N-methylpyrrolidone