Up-regulation of COX2 expression by uni-axial cyclic stretch in human lung fibroblast cells.
The effect of uni-axial cyclic mechanical stretch on the expression of cyclooxygenases (COX) was investigated in a human lung fibroblast cell line (TIG-1). In response to uni-axial cyclic stretch, the level of COX2 mRNA significantly increased and peaked at 3 h (9.09 +/- 3.82-fold, mean +/- standard error, n = 6, compared with that at 1 h). The level of the expression of COX2 protein peaked at 6 h, whereas the level of COX1 protein was not significantly changed. The involvement of stretch-activated (SA) channel was investigated in the stretch-induced COX2 production. The application of Gd3-, a blocker for SA channel, or the removal of extracellular Ca2+ inhibited the production of COX2 mRNA without any effect on the production of COX1 or GAPDH mRNA. These data strongly suggest that COX2 expression is up-regulated by uni-axial cyclic stretch via the activation of SA channel in human lung fibroblasts.
Kato
T
T
Department of Orthopedics, Nagoya University School of Medicine, Japan.
Ishiguro
N
N
Iwata
H
H
Kojima
T
T
Ito
T
T
Naruse
K
K
D002118:Calcium / Q000378:metabolism; D015220:Calcium Channels / Q000187:drug effects / Q000378:metabolism*; D002460:Cell Line; D051545:Cyclooxygenase 1; D051546:Cyclooxygenase 2; D005347:Fibroblasts / Q000166:cytology / Q000201:enzymology; D005682:Gadolinium / Q000494:pharmacology; D006801:Humans; D015640:Ion Channel Gating; D007527:Isoenzymes / Q000096:biosynthesis* / Q000235:genetics; D008168:Lung / Q000166:cytology / Q000201:enzymology*; D008565:Membrane Proteins; D010507:Periodicity; D010812:Physical Stimulation; D011451:Prostaglandin-Endoperoxide Synthases / Q000096:biosynthesis* / Q000235:genetics; D014176:Protein Biosynthesis; D012333:RNA, Messenger / Q000032:analysis; D015398:Signal Transduction; D013312:Stress, Physiological / Q000378:metabolism*; D014158:Transcription, Genetic; D015854:Up-Regulation
D015220:Calcium Channels; D007527:Isoenzymes; D008565:Membrane Proteins; D012333:RNA, Messenger; D005682:Gadolinium; D051545:Cyclooxygenase 1; D051546:Cyclooxygenase 2; C496511:PTGS1 protein, human; C496540:PTGS2 protein, human; D011451:Prostaglandin-Endoperoxide Synthases; D002118:Calcium