Chromosomal localization of a human deoxyribonuclease II gene (DNASE2) to 19p13.2-p13.1 using both the polymerase chain reaction and fluorescence in situ hybridization analysis.
Recently obtained information on the cDNA encoding human deoxyribonuclease II (DNase II) (T. Yasuda et al., 1998, J. Biol. Chem. 273, 2610-2616) has made it possible to demonstrate the precise position of the the human DNase II gene (DNASE2) on human chromosomes. Two different sets of oligonucleotide primers specific for human DNase II cDNA sequences were used to amplify unique DNA fragments in the human DNase II gene from a panel of human x rodent hybrid cell lines carrying different human chromosomes. Based on this analysis, DNASE2 was assigned to human chromosome 19. Furthermore, regional localization of the gene to 19p13.2-p13.1 was achieved by fluorescence in situ hybridization analysis using a full-length cDNA probe corresponding to the entire open reading frame.
Yasuda
T
T
Department of Legal Medicine, Gunma University School of Medicine, Japan.
Takeshita
H
H
Iida
R
R
Nakajima
T
T
Hosomi
O
O
Nakashima
Y
Y
Mogi
K
K
Kishi
K
K
D002874:Chromosome Mapping / Q000379:methods; D002888:Chromosomes, Human, Pair 19; D004706:Endodeoxyribonucleases / Q000235:genetics*; D006801:Humans; D006822:Hybrid Cells; D017404:In Situ Hybridization, Fluorescence; D008938:Mitosis; D016133:Polymerase Chain Reaction
D004706:Endodeoxyribonucleases; C019329:deoxyribonuclease II