Development and applications of enhanced green fluorescent protein mutants.
The introduction of several mutations resulted in the generation of improved mutants of the green fluorescent protein (GFP). A strong green (GFPsg25) and blue (BFPsg50) fluorescent protein, gave 50-fold-100-fold brighter fluorescence compared to wild-type GFP and BFP (Tyr66His), respectively, upon expression in mammalian cells. GFPsg25 and BFPsg50 have different excitation and emission maxima. This allows their use as an efficient dual-color tagging system and their independent detection in living cells.
Stauber
R H
RH
National Cancer Institute-FCRDC, Frederick, MD, USA.
Horie
K
K
Carney
P
P
Hudson
E A
EA
Tarasova
N I
NI
Gaitanaris
G A
GA
Pavlakis
G N
GN
D016475:3T3 Cells; D019943:Amino Acid Substitution / Q000235:genetics; D000818:Animals; D015415:Biomarkers; D005434:Flow Cytometry / Q000295:instrumentation / Q000379:methods; D005786:Gene Expression Regulation; D017930:Genes, Reporter; D049452:Green Fluorescent Proteins; D008164:Luminescent Proteins / Q000096:biosynthesis / Q000235:genetics*; D051379:Mice; D009154:Mutation / Q000235:genetics*; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis / Q000235:genetics; D013050:Spectrometry, Fluorescence; D014162:Transfection
D015415:Biomarkers; D008164:Luminescent Proteins; D011993:Recombinant Fusion Proteins; D049452:Green Fluorescent Proteins