title: Polar localization of the replication origin and terminus in Escherichia coli nucleoids during chromosome partitioning.
abstract: We show the intracellular localization of the Escherichia coli replication origin (oriC) and chromosome terminus during the cell division cycle by FISH. In newborn cells, oriC is localized at the old-pole-proximal nucleoid border and the terminus at the new-pole-proximal nucleoid border. One copy of replicated oriC migrates rapidly to the opposite nucleoid border. These oriC copies are retained at both nucleoid borders, remaining at a constant distance from each cell pole. The terminus segment migrates from the nucleoid border to midcell and is retained there until the terminus is duplicated. The origin, terminus and other DNA regions show three migration patterns during active partitioning of daughter chromosomes.
authors.affiliation: Department of Molecular Cell Biology, Institute of Molecular Embryology and Genetics, Kumamoto University School of Medicine, Kumamoto 862-0976, Japan.
authors.forename: H
authors.initials: H
authors.lastname: Niki
authors.forename: S
authors.initials: S
authors.lastname: Hiraga
mesh_terms: D002453:Cell Cycle; D002875:Chromosomes / Q000502:physiology; D004269:DNA, Bacterial / Q000502:physiology*; D004926:Escherichia coli / Q000166:cytology / Q000502:physiology*; D017404:In Situ Hybridization, Fluorescence; D018741:Replication Origin / Q000502:physiology*
chemical_list: D004269:DNA, Bacterial