title: Development of reference procedures for broth microdilution antifungal susceptibility testing of yeasts with standardized endpoint determination.
abstract: Standard guidelines for the broth microdilution antifungal susceptibility testing of amphotericin B, flucytosine, fluconazole, miconazole and itraconazole are reported. These are a modification of the method developed by the National Committee for Clinical Laboratory Standards (NCCLS) on the following two points: standardization of the means of endpoint determination and the inclusion of miconazole and itraconazole in the testing. MIC was determined to be when the positive control had a turbidity of 0.2 at the 630 nm wavelength. The endpoint was 80% inhibition for azoles and 100% inhibition for other drugs. The method provided good reproducibility, and a wide range of MIC distribution was observed in all antifungal agents except amphotericin B.
authors.affiliation: Teikyo University Institute of Medical Mycology, Hachioji, Tokyo, Japan. makimura@main.teikyo-u.ac.jp
authors.forename: K
authors.initials: K
authors.lastname: Makimura
authors.forename: T
authors.initials: T
authors.lastname: Sudo
authors.forename: M
authors.initials: M
authors.lastname: Kudo
authors.forename: K
authors.initials: K
authors.lastname: Uchida
authors.forename: H
authors.initials: H
authors.lastname: Yamaguchi
mesh_terms: D000935:Antifungal Agents / Q000494:pharmacology*; D002175:Candida / Q000187:drug effects*; D002176:Candida albicans / Q000187:drug effects; D003455:Cryptococcus neoformans / Q000187:drug effects*; D008826:Microbial Sensitivity Tests / Q000592:standards*; D012015:Reference Standards; D015203:Reproducibility of Results
chemical_list: D000935:Antifungal Agents