Trehalose as osmoprotectant in Rhodobacter sphaeroides f. sp. denitrificans IL106.
The photosynthetic bacterium Rhodobacter sphaeroides f. sp. denitrificans IL106 can grow in high osmolarity. The accumulation of intracellular inorganic ions and organic solutes in cells grown in a synthetic medium containing different concentrations of NaCl was examined. Together with potassium ion, trehalose was the major organic osmoprotectant and its accumulation depended on the external salt concentration. Intracellular levels of glycine betaine and other osmoprotectants such as proline did not change when osmolarity increased.
Xu
X
X
Department of Applied Biological Chemistry, University of Tokyo, Japan.
Abo
M
M
Okubo
A
A
Yamazaki
S
S
D001622:Betaine / Q000032:analysis; D002250:Carbon Radioisotopes; D002851:Chromatography, High Pressure Liquid; D005583:Fourier Analysis; D009682:Magnetic Resonance Spectroscopy; D009994:Osmolar Concentration; D011188:Potassium / Q000032:analysis; D012242:Rhodobacter sphaeroides / Q000187:drug effects / Q000254:growth & development* / Q000502:physiology; D012965:Sodium Chloride / Q000494:pharmacology*; D013054:Spectrophotometry, Atomic; D014199:Trehalose / Q000032:analysis / Q000494:pharmacology*
D002250:Carbon Radioisotopes; D001622:Betaine; D012965:Sodium Chloride; D014199:Trehalose; D011188:Potassium