title: Physical identification of a chromosomal locus encoding biosynthetic genes for the lipopeptide calcium-dependent antibiotic (CDA) of Streptomyces coelicolor A3(2).
abstract: Putative peptide-synthetase-encoding DNA fragments were isolated from the Streptomyces coelicolor A3(2) chromosome using a PCR-based approach and mapped to a single approximately 35 kb segment. In integrative transformation experiments, DNA fragments from this region disrupted production of the calcium-dependent antibiotic (CDA) and had sequences characteristic of non-ribosomal peptide synthetases, thus proving that the cda locus had been cloned.
authors.affiliation: Department of Biochemistry and Applied Molecular Biology, University of Manchester Institute of Science and Technology (UMIST), PO Box 88, Manchester M60 1QD, UK.
authors.forename: Pei Pei
authors.initials: PP
authors.lastname: Chong
authors.affiliation: Department of Biochemistry and Applied Molecular Biology, University of Manchester Institute of Science and Technology (UMIST), PO Box 88, Manchester M60 1QD, UK.
authors.forename: Sylvia M
authors.initials: SM
authors.lastname: Podmore
authors.affiliation: John Innes Centre, Norwich NR4 7UH, UK.
authors.forename: Helen M
authors.initials: HM
authors.lastname: Kieser
authors.affiliation: Department of Genetics, Genome Research Unit, Kaiserslautern University, 67663 Kaiserslautern, Germany.
authors.forename: Matthias
authors.initials: M
authors.lastname: Redenbach
authors.affiliation: FB Chemie, Philipps-Universität, Hans-Meerwein-Strasse, 35032 Marburg, Germany.
authors.forename: Kürsad
authors.initials: K
authors.lastname: Turgay
authors.affiliation: FB Chemie, Philipps-Universität, Hans-Meerwein-Strasse, 35032 Marburg, Germany.
authors.forename: Mohamed
authors.initials: M
authors.lastname: Marahiel
authors.affiliation: John Innes Centre, Norwich NR4 7UH, UK.
authors.forename: David A
authors.initials: DA
authors.lastname: Hopwood
authors.affiliation: Department of Biochemistry and Applied Molecular Biology, University of Manchester Institute of Science and Technology (UMIST), PO Box 88, Manchester M60 1QD, UK.
authors.forename: Colin P
authors.initials: CP
authors.lastname: Smith
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D002874:Chromosome Mapping; D002876:Chromosomes, Bacterial; D017931:DNA Primers; D004269:DNA, Bacterial / Q000096:biosynthesis / Q000302:isolation & purification; D005798:Genes, Bacterial; D007476:Ionophores / Q000378:metabolism; D008970:Molecular Weight; D010453:Peptide Synthases / Q000235:genetics* / Q000378:metabolism; D010455:Peptides; D016133:Polymerase Chain Reaction; D015183:Restriction Mapping; D016415:Sequence Alignment; D013302:Streptomyces / Q000235:genetics* / Q000378:metabolism*; D014169:Transformation, Bacterial
chemical_list: C040487:CDA antibiotic; D017931:DNA Primers; D004269:DNA, Bacterial; D007476:Ionophores; D010455:Peptides; D010453:Peptide Synthases