title: Alginate, inorganic polyphosphate, GTP and ppGpp synthesis co-regulated in Pseudomonas aeruginosa: implications for stationary phase survival and synthesis of RNA/DNA precursors.
abstract: The regulatory protein AlgR2 in Pseudomonas aeruginosa positively regulates nucleoside diphosphate kinase (Ndk) and succinyl-CoA synthetase, enzymes critical in nucleoside triphosphate (NTP) formation. AlgR2 positively regulates the production of alginate, GTP, ppGpp and inorganic polyphosphate (poly P). An algR2 mutant with low levels of these metabolites has them restored by introducing and overexpressing either the algR2 or the ndk gene into the algR2 mutant. Thus, Ndk is involved in the formation of these compounds and largely prevents the death of the algR2 mutant, which occurs early in the stationary phase. We demonstrate that the 12 kDa Ndk-pyruvate kinase (Pk) complex, previously shown to generate predominantly GTP instead of all the NTPs, has a low affinity for the deoxynucleoside diphosphates and cannot generate the dNTPs needed for DNA replication and cell division; this complex may thus be involved in regulating the levels of both NTPs and dNTPs that modulate cell division and survival in the stationary phase.
authors.affiliation: Department of Biochemistry, Beckman Center, Stanford University School of Medicine, CA 94305-5307, USA.
authors.forename: H Y
authors.initials: HY
authors.lastname: Kim
authors.forename: D
authors.initials: D
authors.lastname: Schlictman
authors.forename: S
authors.initials: S
authors.lastname: Shankar
authors.forename: Z
authors.initials: Z
authors.lastname: Xie
authors.forename: A M
authors.initials: AM
authors.lastname: Chakrabarty
authors.forename: A
authors.initials: A
authors.lastname: Kornberg
mesh_terms: D000464:Alginates / Q000378:metabolism*; D001426:Bacterial Proteins / Q000187:drug effects / Q000235:genetics / Q000378:metabolism; D004269:DNA, Bacterial / Q000096:biosynthesis; D015964:Gene Expression Regulation, Bacterial; D020723:Glucuronic Acid; D006159:Guanosine Tetraphosphate / Q000096:biosynthesis*; D006160:Guanosine Triphosphate / Q000096:biosynthesis*; D006603:Hexuronic Acids; D009701:Nucleoside-Diphosphate Kinase / Q000235:genetics / Q000378:metabolism; D010710:Phosphates / Q000378:metabolism / Q000494:pharmacology; D011122:Polyphosphates / Q000378:metabolism; D011401:Promoter Regions, Genetic; D011494:Protein Kinases / Q000187:drug effects / Q000235:genetics / Q000378:metabolism; D011550:Pseudomonas aeruginosa / Q000235:genetics* / Q000378:metabolism*; D012322:RNA Precursors / Q000096:biosynthesis; D015534:Trans-Activators
chemical_list: C079502:AlgQ protein, Pseudomonas aeruginosa; D000464:Alginates; D001426:Bacterial Proteins; D004269:DNA, Bacterial; D006603:Hexuronic Acids; D010710:Phosphates; D011122:Polyphosphates; D012322:RNA Precursors; D015534:Trans-Activators; D006159:Guanosine Tetraphosphate; D006160:Guanosine Triphosphate; D020723:Glucuronic Acid; D011494:Protein Kinases; D009701:Nucleoside-Diphosphate Kinase