A new approach to separate the genus Photobacterium from Vibrio with RFLP patterns by HhaI digestion of PCR-amplified 16S rDNA.
A new approach to separate members of the genus Photobacterium from the genus Vibrio with RFLP (Restriction Fragment Length Polymorphism) patterns by HhaI digestion of PCR-amplified 16S rDNA was developed in the present study. It was clearly shown that these patterns of the genus Photobacterium were unique and distinguishable from Vibrio species. This method is very simple and does not need other supporting procedures, such as Southern transfer and probe hybridization. It can be applied not only to luminous species, but also to non-luminous Photobacterium spp. This result promises a rapid tool to distinguish the genus Photobacterium from Vibrio and should be useful in routine identification system.
Urakawa
H
H
Ocean Research Institute, University of Tokyo, 1-15-1, Minamidai, Nakano-ku, Tokyo 164, Japan.
Kita-Tsukamoto
K
K
Ohwada
K
K
D004275:DNA, Ribosomal / Q000032:analysis / Q000235:genetics* / Q000302:isolation & purification; D015252:Deoxyribonucleases, Type II Site-Specific / Q000378:metabolism*; D005784:Gene Amplification; D010776:Photobacterium / Q000737:chemistry / Q000145:classification* / Q000235:genetics; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length; D012336:RNA, Ribosomal, 16S / Q000235:genetics*; D014733:Vibrio / Q000737:chemistry / Q000145:classification* / Q000235:genetics
D004275:DNA, Ribosomal; D012336:RNA, Ribosomal, 16S; D015252:Deoxyribonucleases, Type II Site-Specific; C037130:GCGC-specific type II deoxyribonucleases