The expression of a grapefruit gene encoding an isoflavone reductase-like protein is induced in response to UV irradiation.
Exposure of harvested grapefruit to UV-C (254 nm) irradiation was previously found to induce resistance against the green mold decay caused by Penicillium digitatum. In order to gain insight into the mechanism of this UV-induced resistance we initiated a study for isolation of genes induced during this process. Using the differential display method we cloned cDNA representing an mRNA which is accumulated in grapefruit peel upon UV irradiation. Sequence analysis revealed that this cDNA represents a gene encoding for an isoflavone reductase-like protein and was termed IRL (isoflavone reductase-like). The grapefruit IRL protein sequence has high homology also to a novel family of other isoflavone reductase-like proteins present in few non-legume plants and whose function is not clear yet. The UV dose, and time following it, which lead to maximal accumulation of the IRL transcript were found to be similar to those leading to maximal induced resistance. The expression of the IRL gene was demonstrated to be induced also by wounding and pathogen infection.
Lers
A
A
Department of Postharvest Science of Fresh Produce, The Volcani Center, Bet Dagan, Israel.
Burd
S
S
Lomaniec
E
E
Droby
S
S
Chalutz
E
E
D000595:Amino Acid Sequence; D001483:Base Sequence; D015139:Blotting, Southern; D002352:Carrier Proteins / Q000235:genetics*; D002957:Citrus / Q000201:enzymology / Q000235:genetics* / Q000276:immunology / Q000528:radiation effects; D003001:Cloning, Molecular; D018076:DNA, Complementary; D018744:DNA, Plant / Q000528:radiation effects; D015971:Gene Expression Regulation, Enzymologic / Q000528:radiation effects*; D018506:Gene Expression Regulation, Plant / Q000528:radiation effects*; D007113:Immunity, Innate / Q000528:radiation effects; D047908:Intracellular Signaling Peptides and Proteins; D008969:Molecular Sequence Data; D010088:Oxidoreductases / Q000235:genetics*; D010940:Plant Proteins / Q000235:genetics*; D016415:Sequence Alignment; D014466:Ultraviolet Rays
D002352:Carrier Proteins; D018076:DNA, Complementary; D018744:DNA, Plant; C490892:IRL protein, Citrus x paradisi; D047908:Intracellular Signaling Peptides and Proteins; D010940:Plant Proteins; D010088:Oxidoreductases