title: Survival of transfused CD18-positive granulocytes and their chemiluminescent response in a heifer with leukocyte adhesion deficiency.
abstract: Granulocyte transfusion (GT) was performed in an 8-month-old heifer with leukocyte adhesion deficiency (BLAD) to monitor the changes in transfused CD18-positive neutrophils and associated neutrophil chemiluminescent (CL) response in beta 2-integrin-deficient host. The CD18-positive neutrophils were detected in blood from the BLAD heifer during the first 3 hr after 2.6 x 10(9) cells were infused by GT, and disappeared by 5 hr after GT. The CL response of neutrophils was increased 1.7 to 2.8-fold in the BLAD heifer during the first 3 hr after GT, thereafter CL response decreased gradually from 2 to 5 hr after GT.
authors.affiliation: Department of Animal Health, School of Veterinary Medicine, Rakuno Gakuen University, Ebetsu, Hokkaido, Japan.
authors.forename: H
authors.initials: H
authors.lastname: Nagahata
authors.forename: N
authors.initials: N
authors.lastname: Sakurai
authors.forename: S
authors.initials: S
authors.lastname: Matsuki
authors.forename: T
authors.initials: T
authors.lastname: Miura
authors.forename: K
authors.initials: K
authors.lastname: Nioka
authors.forename: G J
authors.initials: GJ
authors.lastname: Kociba
mesh_terms: D000818:Animals; D015415:Biomarkers; D018821:CD18 Antigens / Q000097:blood*; D002417:Cattle; D002418:Cattle Diseases; D002470:Cell Survival; D005260:Female; D017708:Leukocyte Transfusion / Q000662:veterinary*; D018370:Leukocyte-Adhesion Deficiency Syndrome / Q000628:therapy / Q000662:veterinary*; D008163:Luminescent Measurements; D009504:Neutrophils / Q000166:cytology / Q000276:immunology / Q000502:physiology*
chemical_list: D015415:Biomarkers; D018821:CD18 Antigens