Effects of mesenteric ischemia and reperfusion on small bowel electrical activity.
Previous studies involving basic electrical rhythm (BER) have not been carried out far enough to fully characterize the relationship between mesenteric ischemia and BER. The phenomenon of reperfusion injury has also not been correlated with BER activity. The goal of this study was to characterize changes in BER during mesenteric ischemia and reperfusion and to correlate them with changes in pathology.


METHODS
Serosal electrodes were used to record the electrical activity of rabbit jejunum (n = 20) at baseline, during ischemia (90-210 min), and during reperfusion (120-240 min). BER frequency and amplitude were monitored, and biopsies were taken at the end of ischemia and reperfusion. A pathologist blinded to the specimen identity graded the histology on a scale of 0 (no changes) to 6 (transmural necrosis). Paired t test, the Kruskal-Wallis test of non-parametric ranks, and Fisher's r to z test were used for statistical significance where appropriate.


RESULTS
BER frequency and amplitude fell significantly after 15 min of ischemia and became undetectable by 90 min of ischemia in all animals. The likelihood that BER would return during reperfusion was highly correlated with length of ischemia (r = 0.99). Longer periods of reperfusion were associated with increasing pathologic grade.


CONCLUSIONS
BER frequency and amplitude are very sensitive to ischemia and their changes occur well before histopathologic changes. The variation in electrical activity of the small bowel during ischemia and reperfusion is a dynamic process that reflects the metabolic state of the smooth muscle. If electrical activity of the bowel is to be used for assessment of viability, continuous recordings more accurately reflect the metabolic state of the smooth muscle.
Hegde
S S
SS
Department of Surgery, Vanderbilt University School of Medicine, Nashville, Tennessee, USA.
Seidel
S A
SA
Ladipo
J K
JK
Bradshaw
L A
LA
Halter
S
S
Richards
W O
WO
D000818:Animals; D004195:Disease Models, Animal; D004594:Electrophysiology; D007421:Intestine, Small / Q000098:blood supply* / Q000293:injuries / Q000503:physiopathology*; D007511:Ischemia / Q000175:diagnosis / Q000473:pathology / Q000503:physiopathology*; D009130:Muscle, Smooth / Q000503:physiopathology; D010507:Periodicity; D011817:Rabbits; D015427:Reperfusion Injury / Q000175:diagnosis / Q000473:pathology / Q000503:physiopathology*; D013152:Splanchnic Circulation; D013997:Time Factors