Short term effect of steroids on catecholamine secretion from bovine adrenal medulla chromaffin cells.
Bovine chromaffin cells were used to examine neuronal modulation, as their function is similar to sympathetic post-ganglionic neurons. The effect of steroids on evoked catecholamine secretion from primary culture of bovine adrenal medullary cells was investigated. A wide range of progestins, androgens and estrogens was found to have a significant effect on catecholamine secretion induced by the natural neurotransmitter acetylcholine (ACh). The androgens (especially androstandione and androsterone), as a class were the most effective in inhibition of stimulated secretion, while the estrogens had little, to no, effect. Among all steroids tested, progesterone had the most significant effect, other progestins were less potent. Progesterone inhibited catecholamine secretion evoked by ACh, nicotine and oxotremorine-M in a dose-dependent manner with similar IC50 values in the microM range. It also blocked the secretion evoked by high potassium concentration (59 nM) or veratradine (100 microM), but no effect was seen on the secretion evoked by the calcium ionophore A-23187 (10 microM). Progesterone inhibition of ACh or oxotremorine-M stimulation was immediate and sustained. These results suggest that progesterone and other steroids might have a membrane effect probably acting through blockade of calcium influx necessary for the secretory response.
Dar
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Department of Clinical Biochemistry, Rambam Medical Center, Haifa, Israel. ddar@irp.nida.nih.gov
Zinder
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D000313:Adrenal Medulla / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D000818:Animals; D002395:Catecholamines / Q000378:metabolism*; D002417:Cattle; D002469:Cell Separation; D019439:Chromaffin Cells / Q000187:drug effects / Q000378:metabolism*; D007473:Ion Channels / Q000187:drug effects / Q000378:metabolism; D007700:Kinetics; D009419:Nerve Tissue Proteins / Q000378:metabolism; D011374:Progesterone / Q000494:pharmacology; D013256:Steroids / Q000494:pharmacology*
D002395:Catecholamines; D007473:Ion Channels; D009419:Nerve Tissue Proteins; D013256:Steroids; D011374:Progesterone