title: Application of nitrite reductase from Alcaligenes faecalis S-6 for nitrite measurement.
abstract: The enzymatic reaction of nitrite reductase (NIR) from Alcaligenes faecalis S-6 was applied to the measurement of nitrite. NIR was immobilized on the surface of a gold electrode using filter paper and a dialysis membrane, and used as a working electrode in a three-electrode system. Amperometric methods were applied using NIR and the electron mediator 1-methoxy PMS (1-methoxy-5-methylphenazinium methylsulfate). The decrease in cathodic current showed a correlation to nitrite concentration over the range 0-1 mg/l. Measurements using a batch-flow type system gave a lower detection limit of 0.01 mg/l. This is sufficient for the detection of nitrite in natural waters.
authors.affiliation: Research Center for Advanced Science and Technology, University of Tokyo, Japan.
authors.forename: S
authors.initials: S
authors.lastname: Sasaki
authors.forename: I
authors.initials: I
authors.lastname: Karube
authors.forename: N
authors.initials: N
authors.lastname: Hirota
authors.forename: Y
authors.initials: Y
authors.lastname: Arikawa
authors.forename: M
authors.initials: M
authors.lastname: Nishiyama
authors.forename: M
authors.initials: M
authors.lastname: Kukimoto
authors.forename: S
authors.initials: S
authors.lastname: Horinouchi
authors.forename: T
authors.initials: T
authors.lastname: Beppu
mesh_terms: D000421:Alcaligenes / Q000201:enzymology*; D015374:Biosensing Techniques; D009572:Nitrite Reductases; D009573:Nitrites / Q000032:analysis*; D012680:Sensitivity and Specificity; D014867:Water / Q000032:analysis
chemical_list: D009573:Nitrites; D014867:Water; D009572:Nitrite Reductases