A new family of orphan G protein-coupled receptors predominantly expressed in the brain.
The cloning of a cDNA encoding a G protein-coupled receptor homologous to the endothelin type B receptor, but unable to bind endothelin, was recently reported and termed ET(B)R-LP. We report here the isolation of a human cDNA encoding a receptor that is highly related to ET(B)R-LP and which was therefore termed ET(B)R-LP-2. Comparison of the two amino acid sequences revealed 68% overall homology and 48% identity. As is the case for ET(B)R-LP, the new receptor is strongly expressed in the human central nervous system (e.g. in cerebellar Bergmann glia, cerebral cortex, internal capsule fibers). Membranes of HEK-293 cells stably expressing ET(B)R-LP-2 did not bind endothelin-1, endothelin-2, endothelin-3, bombesin, cholecystokinin-8 or gastrin-releasing peptide.
Valdenaire
O
O
Pharma Division, Preclinical Research, F. Hoffmann-La Roche Ltd., Basel, Switzerland. olivier.valdenaire@roche.com
Giller
T
T
Breu
V
V
Ardati
A
A
Schweizer
A
A
Richards
J G
JG
D000595:Amino Acid Sequence; D001483:Base Sequence; D001667:Binding, Competitive; D015152:Blotting, Northern; D001921:Brain / Q000378:metabolism*; D002490:Central Nervous System / Q000378:metabolism; D018076:DNA, Complementary / Q000302:isolation & purification; D016232:Endothelins / Q000378:metabolism; D019204:GTP-Binding Proteins / Q000378:metabolism*; D006801:Humans; D008969:Molecular Sequence Data; D011506:Proteins / Q000235:genetics* / Q000378:metabolism*; D020794:Receptor Protein-Tyrosine Kinases / Q000235:genetics* / Q000378:metabolism*; D044023:Receptor, Endothelin B; D017466:Receptors, Endothelin / Q000235:genetics / Q000378:metabolism; D043562:Receptors, G-Protein-Coupled; D017386:Sequence Homology, Amino Acid; D014018:Tissue Distribution
D018076:DNA, Complementary; D016232:Endothelins; C111572:GPR37L1 protein, human; D011506:Proteins; D044023:Receptor, Endothelin B; D017466:Receptors, Endothelin; D043562:Receptors, G-Protein-Coupled; D020794:Receptor Protein-Tyrosine Kinases; D019204:GTP-Binding Proteins