Isolation and characterization of 5'-regulatory region of mouse activin beta A subunit gene.
We isolated genomic clones that contain the 5'-flanking region of the mouse activin beta A subunit gene. The nucleotide sequence determination of the 5'-flanking region of the gene and the comparison of that with the reported mouse cDNA structure identified the putative 5' regulatory region, a novel first exon and a part of the first intron of the gene within this region. The putative 5' regulatory region of the mouse activin beta A subunit gene directed the expression of CAT gene in transfected HT1080 cells. Successive deletions of this region demonstrated a 400-bp region that exerts a strong positive effect on promoter activity of the mouse activin beta A subunit gene.
Yoshida
E
E
Institute of Applied Biochemistry, University of Tsukuba, Ibaraki, Japan.
Tanimoto
K
K
Murakami
K
K
Fukamizu
A
A
D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics; D005091:Exons / Q000235:genetics; D005354:Fibrosarcoma; D005796:Genes / Q000235:genetics; D028322:Inhibin-beta Subunits; D007265:Inhibins / Q000235:genetics*; D007438:Introns / Q000235:genetics; D051379:Mice; D008810:Mice, Inbred C57BL; D008969:Molecular Sequence Data; D011401:Promoter Regions, Genetic / Q000235:genetics*; D011993:Recombinant Fusion Proteins; D017422:Sequence Analysis, DNA; D017384:Sequence Deletion; D014158:Transcription, Genetic / Q000235:genetics; D014407:Tumor Cells, Cultured
D018076:DNA, Complementary; D011993:Recombinant Fusion Proteins; C103008:inhibin beta A subunit; D007265:Inhibins; D028322:Inhibin-beta Subunits