Studies on mercury-detoxicating enzymes from a broad-spectrum mercury-resistant strain of Flavobacterium rigense.
Flavobacterium rigense strain PR2, a broad-spectrum mercury-resistant bacterium abundantly present in soil exhibited multiple metal resistance properties. Mercury resistance was due to the sequential action of two mercury-detoxicating enzymes, organomercurial lyase and mercuric reductase. The levels of these enzyme activities were determined using different mercury compounds as inducers and substrates. Mercuric reductase was partially purified from the bacterium and the physicochemical properties of the enzyme were studied. The effect of several enzyme inhibitors and heavy metal ions on the enzyme activity was also studied.
Gachhui
R
R
Department of Biochemistry, University College of Science, Calcutta University, India.
Chaudhuri
J
J
Ray
S
S
Pahan
K
K
Mandal
A
A
D001426:Bacterial Proteins / Q000037:antagonists & inhibitors / Q000302:isolation & purification / Q000378:metabolism*; D004352:Drug Resistance, Microbial; D004791:Enzyme Inhibitors / Q000494:pharmacology; D005417:Flavobacterium / Q000187:drug effects / Q000201:enzymology* / Q000302:isolation & purification; D008190:Lyases / Q000378:metabolism*; D008628:Mercury / Q000494:pharmacology*; D009941:Organomercury Compounds / Q000378:metabolism / Q000494:pharmacology*; D010088:Oxidoreductases / Q000037:antagonists & inhibitors / Q000302:isolation & purification / Q000378:metabolism*; D010573:Pesticide Residues / Q000378:metabolism; D012988:Soil Microbiology; D012989:Soil Pollutants / Q000378:metabolism
D001426:Bacterial Proteins; D004791:Enzyme Inhibitors; D009941:Organomercury Compounds; D010573:Pesticide Residues; D012989:Soil Pollutants; D010088:Oxidoreductases; C021551:mercuric reductase; D008190:Lyases; C050953:alkylmercury lyase; D008628:Mercury