title: EGF does not induce Msx-1 and Msx-2 in dental mesenchyme.
abstract: Previous heterospecific tissue recombinations indicate that mandibular epithelium exerts the first known inductive signal for odontogenesis in mouse embryos. BMP-4 and EGF are two growth factors implicated as signaling molecules mediating the initial inductive epithelial-mesenchymal interactions during odontogenesis. The purpose of the present study was to examine and compare the effects of these growth factors and mouse mandibular epithelium on expression of Msx-1 and Msx-2 genes in molar-forming mesenchyme. Agarose beads soaked in growth factors or pieces of mouse mandibular epithelium (E11) were placed in contact with E11 molar-forming mesenchyme and cultured for 24 h. Whole-mount in situ hybridization analysis revealed that, in contrast to mouse mandibular epithelium and BMP-4-releasing beads, EGF-releasing beads did not induce the expression of Msx-1 and Msx-2 in E11 molar-forming mesenchyme. These observations suggest that whereas BMP-4 may be involved in activation of Msx-1 and Msx-2 in the underlying mesenchyme, EGF may regulate events involved in the formation of dental lamina.
authors.affiliation: Department of BioStructure and Function, School of Dental Medicine, University of Connecticut Health Center, Farmington 06030, USA.
authors.forename: Y H
authors.initials: YH
authors.lastname: Wang
authors.forename: E J
authors.initials: EJ
authors.lastname: Kollar
authors.forename: W B
authors.initials: WB
authors.lastname: Upholt
authors.forename: M
authors.initials: M
authors.lastname: Mina
mesh_terms: D000818:Animals; D055415:Bone Morphogenetic Protein 4; D019485:Bone Morphogenetic Proteins / Q000494:pharmacology / Q000502:physiology; D004268:DNA-Binding Proteins / Q000235:genetics*; D004815:Epidermal Growth Factor / Q000494:pharmacology* / Q000502:physiology; D004848:Epithelium / Q000196:embryology; D018507:Gene Expression Regulation, Developmental / Q000187:drug effects; D018398:Homeodomain Proteins / Q000235:genetics*; D017403:In Situ Hybridization; D051957:MSX1 Transcription Factor; D008334:Mandible / Q000196:embryology; D008648:Mesoderm / Q000187:drug effects; D051379:Mice; D009805:Odontogenesis / Q000187:drug effects* / Q000235:genetics / Q000502:physiology; D011994:Recombinant Proteins / Q000187:drug effects; D015398:Signal Transduction; D014157:Transcription Factors
chemical_list: C525810:Bmp4 protein, mouse; D055415:Bone Morphogenetic Protein 4; D019485:Bone Morphogenetic Proteins; D004268:DNA-Binding Proteins; D018398:Homeodomain Proteins; D051957:MSX1 Transcription Factor; C084119:MSX2 protein; D011994:Recombinant Proteins; D014157:Transcription Factors; D004815:Epidermal Growth Factor