Protein synthesis is involved in the modulation of the level of the phosphoenolpyruvate carboxykinase mRNA by changes in cell volume in isolated rat hepatocytes.
The mechanism of action of hydration state was studied on phosphoenolpyruvate carboxykinase (PCK) gene expression in isolated rat hepatocytes. Hypoosmolarity decreased the level of the PCK mRNA after a lag period of about 60 min. The decreasing effect of hypoosmolarity was totally blocked by inhibitors of both protein synthesis and gene transcription. Moreover, hypoosmolarity specifically increased the synthesis of a 45000 Mr protein, which decreased in the presence of inhibitors of transcription. A close relationship between the synthesis of the 45000 Mr protein and the decrease in the PCK mRNA level was observed, suggesting that this protein might potentially be involved in the regulation of the level of the PCK mRNA by cell swelling.
Quillard
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M
Groupe de Biochimie et Physiopathologie Digestive et Nutritionnelle, Institut Fédératif de Recherches Multidisciplinaires sur les Peptides No. 23, UFR Médecine-Pharmacie de Rouen, Saint Etienne du Rouvray, France.
Husson
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Chedeville
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Fairand
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Lavoinne
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D000546:Amanitins / Q000494:pharmacology; D000818:Animals; D048429:Cell Size; D003609:Dactinomycin / Q000494:pharmacology; D008099:Liver / Q000166:cytology / Q000187:drug effects / Q000201:enzymology*; D008297:Male; D008970:Molecular Weight; D019384:Nucleic Acid Synthesis Inhibitors / Q000494:pharmacology; D009994:Osmolar Concentration; D010729:Phosphoenolpyruvate Carboxykinase (GTP) / Q000096:biosynthesis* / Q000737:chemistry; D011500:Protein Synthesis Inhibitors / Q000494:pharmacology; D012333:RNA, Messenger / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar
D000546:Amanitins; D019384:Nucleic Acid Synthesis Inhibitors; D011500:Protein Synthesis Inhibitors; D012333:RNA, Messenger; D003609:Dactinomycin; D010729:Phosphoenolpyruvate Carboxykinase (GTP)