title: Alpha beta T cell response to Toxoplasma gondii in previously unexposed individuals.
abstract: The mechanisms by which T cells from previously unexposed hosts respond in vitro to certain intracellular pathogens remain to be fully understood. We report and characterize the in vitro reactivity to Toxoplasma gondii of human alpha beta T cells from T. gondii-seronegative individuals. Resting alpha beta T cells from these individuals proliferated in response to PBMC infected with T. gondii or pulsed with T. gondii lysate Ags. This was accompanied by an increase in the percentage of CD4+ alpha beta T cells. Purified CD4+ alpha beta T cells but not CD8+ alpha beta T cells proliferated in response to these T. gondii preparations. Both CD4+ alpha beta T cells with naive (CD45RA+) and memory (CD45RO+) phenotypes from adults as well as alpha beta T cells from T. gondii-seronegative newborns proliferated after incubation with T. gondii. This alpha beta T cell response to the parasite was inhibited by anti-HLA-DR mAb and to a lesser degree by anti-HLA-DQ mAb. Use of paraformaldehyde-fixed PBMC completely abrogated the proliferation of alpha beta T cells, indicating the need for processing of T. gondii Ags. Analysis of the TCR V beta expression did not show evidence for restriction in TCR V beta usage during T. gondii stimulation of alpha beta T cells. Alpha beta T cells secreted significant amounts of IFN-gamma after incubation with T. gondii-infected monocytes. This rapid and remarkable alpha beta T cell response may play an important role in the early events of the immune response to T. gondii.
authors.affiliation: Division of Infectious Diseases, University of Cincinnati College of Medicine, OH 45267, USA. subauscs@email.uc.edu
authors.forename: C S
authors.initials: CS
authors.lastname: Subauste
authors.forename: F
authors.initials: F
authors.lastname: Fuh
authors.forename: R
authors.initials: R
authors.lastname: de Waal Malefyt
authors.forename: J S
authors.initials: JS
authors.lastname: Remington
mesh_terms: D000328:Adult; D000818:Animals; D017951:Antigen Presentation; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology / Q000469:parasitology; D002478:Cells, Cultured; D006683:HLA-DQ Antigens / Q000276:immunology; D006801:Humans; D007156:Immunologic Memory; D016130:Immunophenotyping; D007231:Infant, Newborn; D007371:Interferon-gamma / Q000096:biosynthesis; D007399:Interphase / Q000276:immunology; D008213:Lymphocyte Activation; D009000:Monocytes / Q000276:immunology / Q000378:metabolism / Q000469:parasitology; D016693:Receptors, Antigen, T-Cell, alpha-beta / Q000096:biosynthesis / Q000276:immunology*; D016176:T-Lymphocyte Subsets / Q000276:immunology* / Q000378:metabolism / Q000469:parasitology*; D014122:Toxoplasma / Q000276:immunology*; D014123:Toxoplasmosis / Q000276:immunology
chemical_list: D006683:HLA-DQ Antigens; D016693:Receptors, Antigen, T-Cell, alpha-beta; D007371:Interferon-gamma