title: Increased transversions in a novel mutator colon cancer cell line.
abstract: We describe a novel mutator phenotype in the Vaco411 colon cancer cell line which increases the spontaneous mutation rate 10-100-fold over background. This mutator results primarily in transversion base substitutions which are found infrequently in repair competent cells. Of the four possible types of transversions, only three were principally recovered. Spontaneous mutations recovered also included transitions and large deletions, but very few frameshifts were recovered. When compared to known mismatch repair defective colon cancer mutators, the distribution of mutations in Vaco411 is significantly different. Consistent with this difference, Vaco411 extracts are proficient in assays of mismatch repair. The Vaco411 mutator appears to be novel, and is not an obvious human homologue of any of the previously characterized bacterial or yeast transversion phenotypes. Several hypotheses by which this mutator may produce transversions are presented.
authors.affiliation: Department of Pathology, University Hospitals of Cleveland and Case Western Reserve University, Ohio 44106, USA.
authors.forename: J R
authors.initials: JR
authors.lastname: Eshleman
authors.forename: P S
authors.initials: PS
authors.lastname: Donover
authors.forename: S J
authors.initials: SJ
authors.lastname: Littman
authors.forename: S E
authors.initials: SE
authors.lastname: Swinler
authors.forename: G M
authors.initials: GM
authors.lastname: Li
authors.forename: J D
authors.initials: JD
authors.lastname: Lutterbaugh
authors.forename: J K
authors.initials: JK
authors.lastname: Willson
authors.forename: P
authors.initials: P
authors.lastname: Modrich
authors.forename: W D
authors.initials: WD
authors.lastname: Sedwick
authors.forename: S D
authors.initials: SD
authors.lastname: Markowitz
authors.forename: M L
authors.initials: ML
authors.lastname: Veigl
mesh_terms: D001482:Base Composition / Q000235:genetics; D001483:Base Sequence; D002460:Cell Line; D003110:Colonic Neoplasms / Q000235:genetics*; D004260:DNA Repair / Q000235:genetics*; D006801:Humans; D007041:Hypoxanthine Phosphoribosyltransferase / Q000235:genetics*; D009154:Mutation; D017354:Point Mutation; D017384:Sequence Deletion; D014407:Tumor Cells, Cultured
chemical_list: D007041:Hypoxanthine Phosphoribosyltransferase