title: Proton gradient-driven nickel uptake by vacuolar membrane vesicles of Saccharomyces cerevisiae.
abstract: A vacuolar H+-ATPase-negative mutant of Saccharomyces cerevisiae was highly sensitive to nickel ion. Accumulation of nickel ion in the cells of this mutant of less than 60% of the value for the parent strain arrested growth, suggesting a role for this ATPase in sequestering nickel ion into vacuoles. An artificially imposed pH gradient (interior acid) induced transient nickel ion uptake by vacuolar membrane vesicles, which was inhibited by collapse of the pH difference but not of the membrane potential. Nickel ion transport into vacuoles in a pH gradient-dependent manner is thus important for its detoxification in yeast.
authors.affiliation: Faculty of Pharmaceutical Sciences, Chiba University, Japan.
authors.forename: K
authors.initials: K
authors.lastname: Nishimura
authors.forename: K
authors.initials: K
authors.lastname: Igarashi
authors.forename: Y
authors.initials: Y
authors.lastname: Kakinuma
mesh_terms: D006863:Hydrogen-Ion Concentration; D008274:Magnesium / Q000494:pharmacology; D009532:Nickel / Q000378:metabolism* / Q000494:pharmacology; D006180:Proton-Translocating ATPases / Q000502:physiology; D012441:Saccharomyces cerevisiae / Q000187:drug effects / Q000378:metabolism*; D014617:Vacuoles / Q000378:metabolism*
chemical_list: D009532:Nickel; D006180:Proton-Translocating ATPases; D008274:Magnesium