title: Nucleotide sequence and molecular characterization of a gene encoding GTP-binding protein from Streptococcus gordonii.
abstract: A 1286-bp fragment of chromosomal DNA from Streptococcus gordonii strain Challis was cloned and sequenced. The gene sgg consisted of 897-bp nucleotides encoding a 299-amino acid polypeptide (33,200 Da). The deduced amino acid sequence exhibited significant similarity to Era, G protein of Escherichia coli. The nucleotide binding assay demonstrated that recombinant Sgg bound [32P]GTP but not [32P]ATP, [32P]CTP, or [32P]UTP. These findings indicate that Sgg is a member of the G protein superfamily in the genus Streptococcus.
authors.affiliation: Department of Oral Microbiology, Osaka University Faculty of Dentistry, Japan. kawabata@dent.osaka-u.ac.jp
authors.forename: S
authors.initials: S
authors.lastname: Kawabata
authors.forename: Y
authors.initials: Y
authors.lastname: Terao
authors.forename: T
authors.initials: T
authors.lastname: Andoh
authors.forename: S
authors.initials: S
authors.lastname: Hamada
mesh_terms: D000595:Amino Acid Sequence; D001426:Bacterial Proteins; D001483:Base Sequence; D003001:Cloning, Molecular; D004269:DNA, Bacterial / Q000032:analysis; D019204:GTP-Binding Proteins / Q000235:genetics*; D015964:Gene Expression Regulation, Bacterial; D005798:Genes, Bacterial; D008969:Molecular Sequence Data; D011994:Recombinant Proteins / Q000032:analysis; D017422:Sequence Analysis, DNA; D013291:Streptococcus / Q000235:genetics*
chemical_list: D001426:Bacterial Proteins; D004269:DNA, Bacterial; D011994:Recombinant Proteins; D019204:GTP-Binding Proteins; C111262:SGG protein, Streptococcus