Suppression subtractive hybridization (SSH) identifies prolactin stimulation of p38 MAP kinase gene expression in Nb2 T lymphoma cells: molecular cloning of rat p38 MAP kinase.
Suppression Subtractive Hybridization (SSH) has been used to compare rat Nb2 cells treated with prolactin for 1 hour with untreated cells. This new method for identifying differentially expressed genes showed that the mRNAs for at least three genes were elevated by such treatment, including a p38 mitogen activated protein (MAP) kinase. The p38 MAP kinase was cloned and the full length cDNA sequence was determined.
Nemeth
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Pacific Biomedical Research Center, University of Hawaii, Honolulu 96822, USA.
Bole-Feysot
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C
Tashima
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LS
D000818:Animals; D015152:Blotting, Northern; D017871:Calcium-Calmodulin-Dependent Protein Kinases / Q000235:genetics*; D003001:Cloning, Molecular; D018076:DNA, Complementary; D015971:Gene Expression Regulation, Enzymologic / Q000187:drug effects*; D016399:Lymphoma, T-Cell / Q000201:enzymology* / Q000473:pathology; D020928:Mitogen-Activated Protein Kinases; D009693:Nucleic Acid Hybridization; D011388:Prolactin / Q000494:pharmacology*; D051381:Rats; D013382:Subtraction Technique; D014407:Tumor Cells, Cultured; D015854:Up-Regulation; D048051:p38 Mitogen-Activated Protein Kinases
D018076:DNA, Complementary; D011388:Prolactin; D017871:Calcium-Calmodulin-Dependent Protein Kinases; D020928:Mitogen-Activated Protein Kinases; D048051:p38 Mitogen-Activated Protein Kinases