Thrombin-sensitive peptide linkers for biological signal-responsive drug release systems.
We have previously reported an elevation of thrombin-like activity in infected wound exudates. Therefore, using this enzymatic activity as a biological signal, a system which can release an antimicrobial drug at infected wounds was investigated. In this paper, we report thrombin-sensitive peptide linkers, the key component of this system. Starting from amino acid sequences of the cleavage site in fibrinogen, which is the substrate of thrombin, we synthesized some thrombin-sensitive peptide linkers. We constructed devices in which the thrombin-sensitive peptide linker interconnected between polyvinyl alcohol hydrogel and gentamicin. The device was able to release gentamicin in response to thrombin.
Tanihara
M
M
Graduate School of Material Science, NARA Institute of Science and Technology, Ikoma, Japan. mtanihar@ms.aist-nara.ac.jp
Suzuki
Y
Y
Nishimura
Y
Y
Suzuki
K
K
Kakimaru
Y
Y
D005839:Gentamicins / Q000008:administration & dosage*; D007931:Leucyl Aminopeptidase / Q000378:metabolism; D010455:Peptides / Q000737:chemistry*; D011142:Polyvinyl Alcohol / Q000737:chemistry; D011355:Prodrugs / Q000737:chemistry*; D013917:Thrombin / Q000378:metabolism*
D005839:Gentamicins; D010455:Peptides; D011355:Prodrugs; D011142:Polyvinyl Alcohol; D007931:Leucyl Aminopeptidase; D013917:Thrombin