A rapid method for the preparation of single-cell suspensions from solid tissues.
A simple and rapid methodology for the preparation of single-cell suspension from animal tissues is described. It uses a device called a "tissue press" to mince the tissue into small pieces of desired sizes which are then dissociated into single cells using a pipette. The device is inexpensive and the method economizes time and labor. An example of its use in DNA double-strand breaks study, using microgel electrophoresis, is described.
Singh
N P
NP
Department of Bioengineering, University of Washington, Seattle 98195-7962, USA. narendra@u.washington.edu
D000818:Animals; D001921:Brain / Q000166:cytology*; D002469:Cell Separation / Q000295:instrumentation* / Q000379:methods; D004247:DNA / Q000032:analysis; D004249:DNA Damage; D004587:Electrophoresis, Agar Gel / Q000379:methods; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley
D004247:DNA