title: Growth hormone stimulates multinucleated cell formation in long-term bone marrow cultures.
abstract: Although the effects of growth hormone on bone metabolism are well-documented, their role in the regulation of immune responses such as the inflammatory process has not been thoroughly explored. This study investigated the formation of multinucleated cells (MNC) in long-term human bone marrow cultures. Experiments using 1 and 100 ng/ml of human recombinant growth hormone (hGH) and 10(-7) M of 1,25 dihydroxyvitamin D3 (VD3) showed that hGH increased the total number and nucleation of MNC. The effects of hGH were generally greater than those observed with VD3. Cytological and immunological characterization of MNC revealed several macrophage polykaryon features. MNC did not respond to calcitonin in a cyclic adenosine monophosphate assay and failed to resorb dentin slices. These results demonstrate that MNC formed in the presence of hGH and VD3 present an essentially macrophage polykaryon phenotype. In this context, growth hormone may be involved in the inflammatory process through upmodulation of macrophage polykaryon formation.
authors.affiliation: Centre de recherche interdisciplinaire sur les tissus calcifiés et les biomatériaux, Faculté de Chirurgie dentaire, Nantes, France.
authors.forename: J
authors.initials: J
authors.lastname: Guicheux
authors.forename: D
authors.initials: D
authors.lastname: Heymann
authors.forename: F
authors.initials: F
authors.lastname: Gouin
authors.forename: P
authors.initials: P
authors.lastname: Pilet
authors.forename: A
authors.initials: A
authors.lastname: Faivre
authors.forename: G
authors.initials: G
authors.lastname: Daculsi
mesh_terms: D000135:Acid Phosphatase / Q000378:metabolism; D000954:Antigens, Surface / Q000096:biosynthesis; D001854:Bone Marrow Cells / Q000166:cytology / Q000187:drug effects* / Q000201:enzymology; D002116:Calcitonin / Q000494:pharmacology; D002117:Calcitriol / Q000494:pharmacology; D002478:Cells, Cultured; D004789:Enzyme Activation / Q000187:drug effects; D015726:Giant Cells / Q000187:drug effects* / Q000201:enzymology; D006651:Histocytochemistry; D019382:Human Growth Hormone / Q000494:pharmacology*; D006801:Humans; D007527:Isoenzymes / Q000378:metabolism; D000071681:Tartrate-Resistant Acid Phosphatase
chemical_list: D000954:Antigens, Surface; D007527:Isoenzymes; D019382:Human Growth Hormone; D002116:Calcitonin; D000135:Acid Phosphatase; D000071681:Tartrate-Resistant Acid Phosphatase; D002117:Calcitriol