Characterization of the 5' flanking region of the human NPT-1 Na+/phosphate cotransporter gene.
To elucidate the expression and regulation of the human type I Na+/phosphate transporter gene (NPT-1), the 5' flanking region of the NPT-1 gene was cloned, and its nucleotide sequence and function were determined. A genomic clone that contained approximately 14.0 kb of the 5'-flanking region of the NPT-1 gene was isolated. A single transcription start site was located 104 base pairs (bp) upstream of the 3' end of exon 1. In addition to the sequence of the 5'-flanking region contained a sequence weakly homologous to a TATA box at position -41 to -36 and many transcriptional regulatory elements. Transient expression revealed that a 45-bp region of proximal to exon 1, which contained TATA-like sequence, was sufficient for promoting luciferase expression in OK-cells derived from opossum kidney proximal tubule.
Taketani
Y
Y
Department of Clinical Nutrition, School of Medicine, University of Tokushima, Japan.
Miyamoto
K
K
Chikamori
M
M
Tanaka
K
K
Yamamoto
H
H
Tatsumi
S
S
Morita
K
K
Takeda
E
E
D002352:Carrier Proteins / Q000737:chemistry / Q000235:genetics* / Q000502:physiology; D003001:Cloning, Molecular; D018387:Codon, Initiator; D006801:Humans; D007684:Kidney Tubules / Q000378:metabolism; D008871:Microvilli / Q000378:metabolism; D008969:Molecular Sequence Data; D011401:Promoter Regions, Genetic / Q000235:genetics*; D050608:Sodium-Phosphate Cotransporter Proteins; D050609:Sodium-Phosphate Cotransporter Proteins, Type I; D027981:Symporters; D014158:Transcription, Genetic
D002352:Carrier Proteins; D018387:Codon, Initiator; C493546:SLC17A1 protein, human; D050608:Sodium-Phosphate Cotransporter Proteins; D050609:Sodium-Phosphate Cotransporter Proteins, Type I; D027981:Symporters