Assessment of subacute mammary inflammation by soluble biomarkers in comparison to somatic cell counts in quarter milk samples from dairy cows.
The usefulness of soluble markers in indirectly differentiating inflamed quarters from healthy quarters of 30 Holstein Friesian cows was tested over a follow-up period of 20 weeks. In comparison to N-acetyl-beta-D-glucosaminidase (NAGase), lactate dehydrogenase (LDH), Na+, K+ and Cl- the somatic cell counts (SCC) were determined in foremilk samples. The soluble parameters were measured by means of an autoanalyzer system (Beckman CX5CE) equipped with solid state ion electrodes. Due to different types of inflammatory reactions it is considered to be advantageous to perform two tests in parallel for assessing the mammary gland status. Cell-damaging processes should best be detected by measuring elevated LDH activity, whereas the impairment of the blood-udder barrier is recorded by the respective elevation of the indicators Na+ and Cl-. Among all variables, SCC included, the soluble parameters showed the least dispersions, whereas those of SCC were the most unfavourable in this respect. Moreover, NAGase/LDH and Na+/Cl- among all variables had the highest correlation coefficients of 0.85 and 0.86, respectively. Threshold values as decision limits are proposed. Additionally, an inter-quarter comparison and evaluation improved the diagnostic value of each indirect mastitis parameter analyzed. By means of discriminant analysis, it is shown that the distribution of inflammation within the udders of this herd was unequal.
Zank
W
W
Staatliches Veterinär- und Lebensmitteluntersuchungsamt, Potsdam, Germany.
Schlatterer
B
B
D000118:Acetylglucosaminidase / Q000032:analysis; D000818:Animals; D015415:Biomarkers; D002417:Cattle; D002452:Cell Count; D016002:Discriminant Analysis; D004573:Electrolytes / Q000032:analysis; D005260:Female; D005500:Follow-Up Studies; D007770:L-Lactate Dehydrogenase / Q000032:analysis; D008414:Mastitis, Bovine / Q000175:diagnosis* / Q000473:pathology; D008892:Milk / Q000737:chemistry* / Q000166:cytology*; D012016:Reference Values
D015415:Biomarkers; D004573:Electrolytes; D007770:L-Lactate Dehydrogenase; D000118:Acetylglucosaminidase