Tailing and 3'-end labeling of RNA with yeast poly(A) polymerase and various nucleotides.
We have tested conditions for the labeling and tailing the 3'-end of RNAs with yeast poly(A) polymerase. Conditions were optimized for addition of NTP, dNTP, or ddNTP nucleotides to RNA. ATP, GTP, and UTP were useful for adding homopolymer tracts of various lengths. The nonradioactive nucleotides biotin-N6-ATP and digoxigenin-11-UTP also were used efficiently.
Martin
G
G
Department of Cell Biology, Biozentrum, University of Basel, Switzerland.
Keller
W
W
D001483:Base Sequence; D001710:Biotin; D004076:Digoxigenin; D008969:Molecular Sequence Data; D009711:Nucleotides / Q000378:metabolism*; D011062:Polynucleotide Adenylyltransferase / Q000378:metabolism*; D012313:RNA / Q000737:chemistry; D016337:RNA, Catalytic / Q000235:genetics / Q000378:metabolism; D012333:RNA, Messenger / Q000737:chemistry* / Q000378:metabolism*; D012441:Saccharomyces cerevisiae / Q000201:enzymology
D009711:Nucleotides; C034477:RNA primers; D016337:RNA, Catalytic; D012333:RNA, Messenger; D012313:RNA; D001710:Biotin; D011062:Polynucleotide Adenylyltransferase; D004076:Digoxigenin