title: A methylation imprint mark in the mouse imprinted gene Grf1/Cdc25Mm locus shares a common feature with the U2afbp-rs gene: an association with a short tandem repeat and a hypermethylated region.
abstract: We identified a sperm-specific methylation imprint mark (Site II) associated with a short tandem repeat sequence and a site/region methylated in both gametes (Site I) in the Grf1 locus on mouse chromosome 9, which shared a common feature with the U2afbp-rs gene. Sites or regions of gamete-specific methylation in imprinted genes are strong candidates for carrying information regarding the parental origin of alleles. The gamete-specific methylation pattern of Sites I and II was conserved after fertilization, but attained the somatic cell pattern by the blastocyst stage. In primordial germ cells, Site I was methylated, but Site II was unmethylated in both male and female embryos, suggesting that the sperm-specific methylation imprint mark in Site II was established during spermatogenesis. These common features in methylation imprint regions may be a clue to identifying regions carrying primary information for the imprinting regulation.
authors.affiliation: Genome Science Laboratory, RIKEN Tsukuba Life Science Center, Ibaraki, Japan.
authors.forename: H
authors.initials: H
authors.lastname: Shibata
authors.forename: Y
authors.initials: Y
authors.lastname: Yoda
authors.forename: R
authors.initials: R
authors.lastname: Kato
authors.forename: T
authors.initials: T
authors.lastname: Ueda
authors.forename: M
authors.initials: M
authors.lastname: Kamiya
authors.forename: N
authors.initials: N
authors.lastname: Hiraiwa
authors.forename: A
authors.initials: A
authors.lastname: Yoshiki
authors.forename: C
authors.initials: C
authors.lastname: Plass
authors.forename: R S
authors.initials: RS
authors.lastname: Pearsall
authors.forename: W A
authors.initials: WA
authors.lastname: Held
authors.forename: M
authors.initials: M
authors.lastname: Muramatsu
authors.forename: H
authors.initials: H
authors.lastname: Sasaki
authors.forename: M
authors.initials: M
authors.lastname: Kusakabe
authors.forename: Y
authors.initials: Y
authors.lastname: Hayashizaki
mesh_terms: D000818:Animals; D001665:Binding Sites; D018797:Cell Cycle Proteins / Q000235:genetics*; D002874:Chromosome Mapping; D019175:DNA Methylation; D047108:Embryonic Development; D005260:Female; D018392:Genomic Imprinting; D020662:Guanine Nucleotide Exchange Factors; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D008969:Molecular Sequence Data; D009419:Nerve Tissue Proteins; D009687:Nuclear Proteins; D010749:Phosphoprotein Phosphatases / Q000235:genetics*; D063146:Polycomb-Group Proteins; D011247:Pregnancy; D011506:Proteins / Q000235:genetics*; D012091:Repetitive Sequences, Nucleic Acid; D012097:Repressor Proteins / Q000235:genetics*; D012261:Ribonucleoproteins / Q000235:genetics*; D000072668:Splicing Factor U2AF; D020663:ras Guanine Nucleotide Exchange Factors; D020666:ras-GRF1
chemical_list: D018797:Cell Cycle Proteins; D020662:Guanine Nucleotide Exchange Factors; D009419:Nerve Tissue Proteins; D009687:Nuclear Proteins; D063146:Polycomb-Group Proteins; D011506:Proteins; D012097:Repressor Proteins; D012261:Ribonucleoproteins; C112628:SCML1 protein, human; D000072668:Splicing Factor U2AF; C000607495:Zrsr1 protein, mouse; D020663:ras Guanine Nucleotide Exchange Factors; D020666:ras-GRF1; D010749:Phosphoprotein Phosphatases