Characterization of a cDNA encoding CuZn-superoxide dismutase from the liverwort Marchantia paleacea var. diptera.
Suspension-cultured cells of the liverwort Marchantia paleacea var. diptera contain a cytosolic CuZn-superoxide dismutase (SOD) whose N-terminal amino acid sequence is similar to those of the isozymes found in chloroplasts of higher plants [Tanaka et al. (1996) Plant Cell Physiol. 37: 523]. A cDNA (MSODCc) encoding the cytosolic CuZn-SOD was isolated from cDNA library constructed from a liverwort cell suspension culture. The deduced amino acid sequence showed a higher degree of homology with the sequences of CuZn-SODs in chloroplasts than those in the cytosol of higher plants and an unique additional peptide in the C-terminal region, but no plastid transit sequence. Northern blotting using MSODCc as a probe and immunoblot analysis with antiserum against the enzyme revealed that the steady state level of transcript was not affected by copper, but both CuZn-SOD protein and its activity increased.
Tanaka
K
K
Department of Biological Science, Faculty of Science, Hiroshima University, Japan.
Takio
S
S
Yamamoto
I
I
Satoh
T
T
D000595:Amino Acid Sequence; D001483:Base Sequence; D002478:Cells, Cultured; D002736:Chloroplasts / Q000201:enzymology; D003600:Cytosol / Q000201:enzymology; D018076:DNA, Complementary; D015723:Gene Library; D008969:Molecular Sequence Data; D010944:Plants / Q000201:enzymology* / Q000235:genetics; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid; D013482:Superoxide Dismutase / Q000096:biosynthesis* / Q000737:chemistry / Q000235:genetics*
D018076:DNA, Complementary; D013482:Superoxide Dismutase