Viremia, genetic heterogeneity, and immunity to hepatitis G/GB-C virus in multiply transfused patients with thalassemia.
BACKGROUND
Thalassemia patients are at high risk for posttransfusion hepatitis. Hepatitis G virus (HGV) has been suspected of being responsible for acute and chronic hepatitis.


METHODS
The prevalence of HGV infection, its possible association to liver disease, the genetic heterogeneity among the various HGV isolates, and immunity to HGV were studied in 36 thalassemia patients with reverse transcriptase-polymerase chain reaction assay and sequence analysis.


RESULTS
HGV RNA was detected in seven patients (19.4%), only two of whom had evidence of hepatitis C virus infection as well. Sequence analysis of the NS3 gene from isolates of the five patients infected with HGV alone revealed 84.7 to 90.9 percent homology at the nucleotide level. Prolonged HGV viremia was not associated with significant liver enzyme elevation. All five patients were chronically infected with the same viral strain. E2 antibodies were detected in 57 percent of the HGV-nonviremic patients and in only 1 of 7 viremic patients.


CONCLUSIONS
HGV is associated with persistent viremia but not with significant biochemical evidence of liver damage. There is some genetic heterogeneity among HGV isolates from thalassemia patients in Israel.
Zemel
R
R
Molecular Hepatology Research Laboratory, Felsenstein Medical Research Center, Tel-Aviv University, Israel.
Dickman
R
R
Tamary
H
H
Bukh
J
J
Zaizov
R
R
Tur-Kaspa
R
R
D000595:Amino Acid Sequence; D000755:Anemia, Sickle Cell / Q000821:virology; D001803:Blood Transfusion; D005260:Female; D018067:Flaviviridae / Q000235:genetics; D006525:Hepatitis, Viral, Human / Q000276:immunology*; D006801:Humans; D008297:Male; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D012367:RNA, Viral / Q000032:analysis; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid; D012689:Sequence Homology, Nucleic Acid; D017086:beta-Thalassemia / Q000628:therapy*
D012367:RNA, Viral