Systemic complement depletion reduces inflammation and demyelination in adoptive transfer experimental allergic neuritis.
The effect of systemic complement depletion by cobra venom factor (CVF) was evaluated in adoptive transfer experimental allergic neuritis (AT-EAN). Spleen cells of rats immunized with a neuritogenic peptide SP26 were injected into naive rats. On days 3 and 6 after cell transfer AT-EAN rats were treated with CVF or saline intraperitoneally. AT-EAN rats treated with CVF had significantly lower scores for histological inflammation (0.25 +/- 0.25 vs 1.9 +/- 0.4, mean +/- SEM, P < 0.03) and demyelination (0.13 +/- 0.13 vs 1.6 +/- 1.4, P < 0.02) than saline-treated AT-EAN rats. Immunocytochemistry of lumbosacral nerve roots showed significantly less ED1-positive macrophages (0.5 +/- 0.3 vs 1.6 +/- 0.6, P < 0.04) and CD11bc-positive (expressing complement receptor 3 or CR3) inflammatory cells (0.6 +/- 0.4 vs 1.7 +/- 0.5, P < 0.03). Our data suggest that complement plays a crucial role in inflammatory demyelination since systemic complement depletion significantly reduces recruitment of macrophages into the nerve and subsequent macrophage-mediated demyelination.
Vriesendorp
F J
FJ
Department of Neurology, University of Texas Health Science Center, Houston 77030, USA. fvriesen@neuro.med.uth.tmc.edu
Flynn
R E
RE
Malone
M R
MR
Pappolla
M A
MA
D000818:Animals; D018845:CD11 Antigens / Q000032:analysis; D002420:Cauda Equina / Q000737:chemistry / Q000473:pathology; D003169:Complement Inactivator Proteins; D003165:Complement System Proteins / Q000276:immunology / Q000378:metabolism*; D003711:Demyelinating Diseases / Q000276:immunology / Q000378:metabolism* / Q000473:pathology; D003713:Dendritic Cells / Q000737:chemistry / Q000276:immunology; D004546:Elapid Venoms; D005260:Female; D007150:Immunohistochemistry; D008264:Macrophages / Q000737:chemistry / Q000276:immunology; D009444:Neuritis, Autoimmune, Experimental / Q000276:immunology / Q000378:metabolism* / Q000473:pathology; D051381:Rats; D011917:Rats, Inbred Lew; D013126:Spinal Nerve Roots / Q000737:chemistry / Q000473:pathology
D018845:CD11 Antigens; D003169:Complement Inactivator Proteins; D004546:Elapid Venoms; C016310:cobra venom factor; D003165:Complement System Proteins