D4S43 locus DNA typing in the Japanese population and application to teeth with degraded DNA.
VNTR polymorphism at the D4S43 locus was investigated, and allele frequencies in the Japanese population sample were determined by the polymerase chain reaction. Eleven different alleles and 16 genotypes were observed in 131 unrelated Japanese. The most common allele was one repeat unit (60.3%), the heterozygosity index of the present study was 58.7%, and the polymorphic information content was calculated at 0.55. In addition, we found four new size variations in the one-repeat-unit allele, which was the most common allele among the Japanese population. These variations were believed to be different from variations by repeats of the basic 14-bp unit. The one-repeat-unit allele at the D4S43 locus, which comes in four sizes, was detected in all of the samples, including degraded DNA samples obtained from hard tooth tissue. Therefore, this new variation is useful in personal identification by DNA analysis using forensic scientific degraded DNA samples.
Hanaoka
Y
Y
Department of Forensic Odontology, Tokyo Dental College, Chiba, Japan.
Minaguch
K
K
D000483:Alleles; D004247:DNA / Q000235:genetics / Q000378:metabolism*; D016172:DNA Fingerprinting; D017931:DNA Primers / Q000737:chemistry; D005553:Forensic Dentistry / Q000379:methods*; D005787:Gene Frequency; D006801:Humans; D007564:Japan; D018598:Minisatellite Repeats / Q000235:genetics*; D016133:Polymerase Chain Reaction; D014070:Tooth / Q000378:metabolism*
D017931:DNA Primers; D004247:DNA