Distribution and characterization of faecal necrotoxigenic Escherichia coli CNF1+ and CNF2+ isolated from healthy cows and calves.
Faecal swabs obtained from a random sample of 268 cows and 90 calves on 19 Lugo (northwestern Spain) farms were examined for necrotoxigenic Escherichia coli (NTEC) producing the cytotoxic necrotizing factors type 1 (CNF1) and type 2 (CNF2). We found NTEC CNF1+ and CNF2+ on 11% and 95% of the farms, respectively, NTEC producing CNF2 were significantly more frequently isolated from calves (58%) than from cows (17%) (P < 0.001). The proportion of animals colonized with CNF2+ strains on each farm ranged from 0% to 60%. NTEC strains producing CNF2 isolated from healthy cattle belonged to 27 O serogroups; however, 64% were of one of 12 serogroups (O2, O8, O8-O75, O14, O15, O55, O86, O88, O115, O121, O147, and O168). Furthermore, the serogroups determined in CNF2+ strains isolated from cows (O2, O8, and O14) were different from those found in NTEC producing CNF2 isolated from calves (O8-O75, O15, O55, O86, O88, O115 and O147).
Blanco
M
M
Department of Microbiology and Parasitology, School of Veterinary Science, University of Santiago de Compostela, Lugo, Spain.
Blanco
J E
JE
Mora
A
A
Blanco
J
J
D000818:Animals; D001427:Bacterial Toxins / Q000032:analysis / Q000096:biosynthesis* / Q000235:genetics; D002417:Cattle; D002418:Cattle Diseases / Q000453:epidemiology / Q000382:microbiology*; D002522:Chlorocebus aethiops; D003603:Cytotoxins / Q000032:analysis / Q000096:biosynthesis* / Q000235:genetics; D017931:DNA Primers / Q000737:chemistry; D004587:Electrophoresis, Agar Gel / Q000662:veterinary; D004926:Escherichia coli / Q000145:classification* / Q000235:genetics / Q000378:metabolism; D004927:Escherichia coli Infections / Q000453:epidemiology / Q000382:microbiology / Q000662:veterinary*; D029968:Escherichia coli Proteins; D005243:Feces / Q000382:microbiology*; D005260:Female; D006367:HeLa Cells; D006801:Humans; D008297:Male; D016133:Polymerase Chain Reaction / Q000662:veterinary; D015995:Prevalence; D012703:Serotyping / Q000662:veterinary; D013030:Spain / Q000453:epidemiology; D014709:Vero Cells
D001427:Bacterial Toxins; D003603:Cytotoxins; D017931:DNA Primers; D029968:Escherichia coli Proteins; C086995:cytotoxic necrotizing factor type 2; C060296:cytotoxic necrotizing factor type 1