Development of a syncytia inhibition assay for the detection of antibodies to bovine leukemia virus in naturally infected cattle; comparison with Western blot and agar gel immunodiffusion.
A syncytia inhibition assay (SIA) for the detection of antibodies to bovine leukemia virus is described. This test involves specific antibody-mediated inhibition of BLV-induced cytopathic effects in an indicator cell line. A total of 300 sera were screened commercially by agar gel immunodiffusion (AGID) and were then screened by Western blot and SIA. The new assay system provided results which were comparable to Western blot and AGID. The results obtained suggest that SIA may be more sensitive than either of the other two assay systems examined for the determination of the infection status of cattle.
Johnson
M
M
Department of Biology, Millersville University, PA 17551, USA.
Rommel
F
F
Moné
J
J
D000818:Animals; D000914:Antibodies, Viral / Q000302:isolation & purification*; D001681:Biological Assay / Q000662:veterinary; D015153:Blotting, Western / Q000379:methods / Q000662:veterinary*; D002417:Cattle; D016583:Enzootic Bovine Leukosis / Q000175:diagnosis / Q000821:virology; D015726:Giant Cells; D005779:Immunodiffusion / Q000379:methods / Q000662:veterinary*; D001909:Leukemia Virus, Bovine / Q000276:immunology*; D012680:Sensitivity and Specificity
D000914:Antibodies, Viral