Chemical shift homology in proteins.
The degree of chemical shift similarity for homologous proteins has been determined from a chemical shift database of over 50 proteins representing a variety of families and folds, and spanning a wide range of sequence homologies. After sequence alignment, the similarity of the secondary chemical shifts of C alpha protons was examined as a function of amino acid sequence identity for 37 pairs of structurally homologous proteins. A correlation between sequence identity and secondary chemical shift rmsd was observed. Important insights are provided by examining the sequence identity of homologous proteins versus percentage of secondary chemical shifts that fall within 0.1 and 0.3 ppm thresholds. These results begin to establish practical guidelines for the extent of chemical shift similarity to expect among structurally homologous proteins.
Potts
B C
BC
Department of Molecular Biology, Scripps Research Institute, La Jolla, CA 92037, USA.
Chazin
W J
WJ
D000818:Animals; D002135:Calcium-Binding Proteins / Q000737:chemistry; D016208:Databases, Factual; D006801:Humans; D009682:Magnetic Resonance Spectroscopy / Q000379:methods*; D011487:Protein Conformation; D011506:Proteins / Q000737:chemistry*; D017386:Sequence Homology, Amino Acid
D002135:Calcium-Binding Proteins; D011506:Proteins