Characterization of the heterochromatic chromosome regions in sheep.
In order to elucidate the structural chromosome organization of the heterochromatic regions in sheep, we have used C-banding, silver-staining, sequential CDD technique and restriction endonuclease banding. By these banding techniques we obtained four fractions of repetitive DNA, the autosomal fractions A and B, the C fraction in the X chromosome, and the D fraction in the Y chromosome. Silver staining revealed active nucleolus organizer regions (NOR's) on the telomeric GC-rich areas of chromosomes 1, 2, 3, 4, and 25 which were digested with HaeIII restriction endonuclease.
Fernández-García
J L
JL
Departamento de Zootecnia, Genética y Mejora Animal, Facultad de Veterinaria, Universidad de Extremadura, Cáceres, Spain.
Martínez-Trancón
M
M
Rabasco
A
A
Padilla
J A
JA
D000818:Animals; D002871:Chromosome Banding; D002875:Chromosomes / Q000737:chemistry*; D004262:DNA Restriction Enzymes; D005260:Female; D006570:Heterochromatin; D007621:Karyotyping; D008297:Male; D009697:Nucleolus Organizer Region; D012756:Sheep / Q000235:genetics*; D016622:Silver Staining; D013194:Staining and Labeling; D016615:Telomere; D014960:X Chromosome / Q000737:chemistry; D014998:Y Chromosome / Q000737:chemistry
D006570:Heterochromatin; D004262:DNA Restriction Enzymes