title: High temperature conjugation of proteins with carbohydrates.
abstract: A new procedure was used to conjugate lactose and dextran with BSA without using coupling or activating reagents. The method is simple, rapid and cheap. Reducing sugars covalently bind to proteins when lyophilized together and briefly heated to a high temperature.
authors.affiliation: Department of Tumor Immunology, Ludwik Hirszfeld Institute of Immunology and Experimental Therapy, Polish Academy of Sciences, Wrocław. borat@immuno.iitd.pan.wroc.pl
authors.forename: J
authors.initials: J
authors.lastname: Boratyński
authors.forename: R
authors.initials: R
authors.lastname: Roy
mesh_terms: D000818:Animals; D003429:Cross Reactions; D003911:Dextrans / Q000138:chemical synthesis / Q000737:chemistry; D006023:Glycoproteins / Q000138:chemical synthesis* / Q000276:immunology*; D007074:Immunoglobulin G / Q000276:immunology; D007785:Lactose / Q000737:chemistry; D008070:Lipopolysaccharides / Q000737:chemistry; D011817:Rabbits; D012710:Serum Albumin, Bovine / Q000138:chemical synthesis / Q000737:chemistry; D013696:Temperature; D014357:Trypsin / Q000737:chemistry
chemical_list: D003911:Dextrans; D006023:Glycoproteins; D007074:Immunoglobulin G; D008070:Lipopolysaccharides; C043578:bovine serum albumin-dextran conjugate; D012710:Serum Albumin, Bovine; D014357:Trypsin; D007785:Lactose