Construction and characterization of nitrate reductase-based amperometric electrode and nitrate assay of fertilizers and drinking water.
The construction and characterization of a nitrate reductase-based amperometric electrode for determination of nitrate ion is described. The electrode consisted of nitrate reductase held by dialysis membrane onto a Nafion-coated glassy carbon electrode. Methyl viologen was allowed to absorb into the Nafion layer, which acted as a reservoir for the electron mediator. The utility of the electrode to assay fertilizer and water sample for nitrate was demonstrated. The assays conducted with this electrode compared well with colorimetric and potentiometric assays of the same samples.
Glazier
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Biotechnology Division, National Institute of Standards and Technology, Gaithersburg, Maryland 20899, USA.
Campbell
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Campbell
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D000042:Absorption; D003124:Colorimetry; D003956:Dialysis; D005308:Fertilizers / Q000032:analysis*; D005465:Fluorocarbon Polymers / Q000737:chemistry; D005618:Fresh Water / Q000032:analysis*; D017736:Ion-Selective Electrodes; D008024:Ligands; D050901:Nitrate Reductase; D009565:Nitrate Reductases / Q000737:chemistry*; D009566:Nitrates / Q000032:analysis*; D011199:Potentiometry / Q000379:methods*
D005308:Fertilizers; D005465:Fluorocarbon Polymers; D008024:Ligands; D009566:Nitrates; C040402:perfluorosulfonic acid; D009565:Nitrate Reductases; D050901:Nitrate Reductase