Inhibitory effect of interleukin-10 on human leukocyte interferon-alpha production by Sendai virus.
Treatment of human peripheral blood leukocytes (hPBL) with Sendai virus induces significant production of human interferon-alpha (IFN-alpha). Addition of human recombinant interleukin-10 (IL-10) to hPBL in vitro prior to treatment with Sendai virus resulted in considerable inhibition of IFN-alpha production. Downregulation of IFN-alpha production was IL-10 concentration-dependent and observed at IL-10 concentrations of as low as 0.05 ng/ml, with a median effective dose (ED50) of about 5 ng/ml. Inhibition of IFN-alpha production by IL-10 occurred at an early stage of Sendai virus induction. The inhibitory effect of IL-10 on leukocyte interferon production was specific and blocked by pretreatment with neutralizing polyclonal anti-IL-10 antibody. This downregulatory effect is at the transcriptional level, since IL-10 inhibits IFN-alpha mRNA accumulation upon Sendai virus treatment. These data suggest that leukocyte IFN-alpha production is a highly regulated process that is modulated by cytokines such as IL-10 during early immunological response to infection.
Zhao
X X
XX
Interferon Sciences Inc, New Brunswick, NJ 08901-3605, USA.
Liao
M J
MJ
Rashidbaigi
A
A
D015536:Down-Regulation; D005786:Gene Expression Regulation; D006801:Humans; D016898:Interferon-alpha / Q000096:biosynthesis*; D016753:Interleukin-10 / Q000494:pharmacology*; D007700:Kinetics; D007962:Leukocytes / Q000187:drug effects*; D018108:Respirovirus / Q000276:immunology*; D014158:Transcription, Genetic / Q000187:drug effects
D016898:Interferon-alpha; D016753:Interleukin-10