title: Detection of infectious simian immunodeficiency virus in B- and T-cell lymphomas of experimentally infected macaques.
abstract: An increasing frequency of malignant lymphomas occurs among patients infected by human immunodeficiency virus. Because of the close similarities to human malignancies, we used a nonhuman primate model to study the pathogenesis of simian immunodeficiency virus (SIV)-associated malignancies. Specifically, we investigated (1) the presence of the SIV genome in tumor cells, (2) the presence of coinfecting viruses, and (3) the presence of a rearrangement of the immunoglobulin and c-myc genes. We observed 5 cases of non-Hodgkin's lymphomas (4 of B- and 1 of T-cell origin) among 14 SIV-infected cynomolgus monkeys. No c-myc translocation was observed in the tumors, whereas B-cell lymphomas were characterized either by a monoclonal (in 2 of 4) or by an oligoclonal (in 2 of 4) VDJ rearrangements of the immunoglobulin heavy chain gene. Molecular, biological, and immunological analyses did show the presence of infectious SIV in the tumor cells of 1 T-cell and 2 oligoclonal B-cell lymphomas. Neither Simian T-lymphotropic nor Epstein-Barr viruses were detectable, whereas Simian herpes virus Macaca fascicularis-1 was detectable at a very low copy number in 3 of 4 B-cell lymphomas; however, only 1 of these also harbored the SIV genome. These results support the possibility that SIV may be directly involved in the process of B or T lymphomagenesis occurring in simian acquired immunodeficiency syndrome.
authors.affiliation: Laboratory of Virology, Istituto Superiore Sanità, Rome, Italy.
authors.forename: M T
authors.initials: MT
authors.lastname: Maggiorella
authors.forename: F
authors.initials: F
authors.lastname: Monardo
authors.forename: M L
authors.initials: ML
authors.lastname: Koanga-Mogtomo
authors.forename: L
authors.initials: L
authors.lastname: Cioè
authors.forename: L
authors.initials: L
authors.lastname: Sernicola
authors.forename: F
authors.initials: F
authors.lastname: Corrias
authors.forename: C D
authors.initials: CD
authors.lastname: Baroni
authors.forename: P
authors.initials: P
authors.lastname: Verani
authors.forename: F
authors.initials: F
authors.lastname: Titti
mesh_terms: D000818:Animals; D000914:Antibodies, Viral / Q000032:analysis; D002999:Clone Cells; D004279:DNA, Viral / Q000235:genetics; D015326:Gene Rearrangement, B-Lymphocyte, Heavy Chain; D016259:Genes, myc; D016393:Lymphoma, B-Cell / Q000473:pathology / Q000821:virology*; D016399:Lymphoma, T-Cell / Q000473:pathology / Q000821:virology*; D008252:Macaca fascicularis; D016097:Simian Acquired Immunodeficiency Syndrome / Q000276:immunology / Q000821:virology*; D015302:Simian Immunodeficiency Virus / Q000254:growth & development*; D015303:Simian T-lymphotropic virus 1 / Q000235:genetics; D014178:Translocation, Genetic
chemical_list: D000914:Antibodies, Viral; D004279:DNA, Viral