Report of a hitherto unreported sequence present and expressed in a cancer cell line.
During the amplification of a 202 base pair (bp) fragment around the 61st codon of the N-ras gene, an extra band of about 150 bp in length was observed when genomic DNA from a human epidermoid carcinoma cell line was used as template. This fragment was cloned and sequenced. However, this sequence was not found in the databank. RT-PCR experiments indicated that the sequence is expressed in the cells about 8 h after serum induction. The relevant RNA hybridized to one strand of the sequence but not to the other.
Das
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Department of Biophysics, Molecular Biology and Genetics, University of Calcutta, India.
Dasgupta
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UB
D001483:Base Sequence; D002294:Carcinoma, Squamous Cell / Q000235:genetics*; D004273:DNA, Neoplasm / Q000032:analysis*; D006801:Humans; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D014407:Tumor Cells, Cultured
D004273:DNA, Neoplasm