A technique for in vivo vascular perfusion fixation of the sheep central nervous system.
A method for perfusion fixation of the sheep central nervous system is described. The technique utilises the vascular anatomy of the spinal cord and brain to provide fixation that is comparable to transcardiac perfusion of the small animal. It can be applied with relative ease, cost effectiveness and without risking premature death of the animal due to complications of a major procedure such as thoracotomy.
Santoreneos
S
S
Department of Neurosurgery, Royal Adelaide Hospital, University of Adelaide, North Terrace, SA, Australia.
Stoodley
M A
MA
Jones
N R
NR
Brown
C J
CJ
D000818:Animals; D001921:Brain / Q000473:pathology; D002339:Carotid Arteries / Q000502:physiology; D002490:Central Nervous System / Q000033:anatomy & histology* / Q000473:pathology; D006259:Craniocerebral Trauma / Q000473:pathology; D010477:Perfusion / Q000379:methods*; D012756:Sheep; D013116:Spinal Cord / Q000473:pathology; D016707:Tissue Fixation / Q000379:methods*