T-cell receptor-gamma rearrangement and c-myb methylation in MNNG-exposed Bloom syndrome B-lymphoblastoid cells.
The MNNG-exposed Bloom syndrome (BS) B-lymphoblastoid cell population (BS-MNNG), when analyzed for aberrant genetic variations, showed an illegitimate rearrangement at the TCR-gamma gene and hypermethylation at the c-myb protooncogene. The TCR-gamma rearrangement involved a Vgamma9 segment corresponding to a 4 kb band detected with a Jgamma-specific probe in HindIII-digested DNA samples from BS-MNNG cells only. These variations were not shown by unexposed BS cells or both MNNG-exposed and unexposed normal (GA3) B-lymphoblastoid cells.
Bhalla
A
A
Human Genetics Laboratory, School of Life Sciences, Jawaharlal Nehru University, New Delhi, India.
Sachdeva
G
G
Bamezai
R
R
D001402:B-Lymphocytes / Q000187:drug effects; D001816:Bloom Syndrome / Q000235:genetics*; D002273:Carcinogens; D002461:Cell Line, Transformed; D002471:Cell Transformation, Neoplastic; D019175:DNA Methylation; D015334:Gene Rearrangement, gamma-Chain T-Cell Antigen Receptor; D006801:Humans; D008769:Methylnitronitrosoguanidine; D009857:Oncogenes
D002273:Carcinogens; D008769:Methylnitronitrosoguanidine