Specific interaction of food proteins with apical membranes of the human intestinal cell lines Caco-2 and T84.
A comparison between the intestinal epithelial cell lines Caco-2 and T84 was made to assess the influence of enterocytic differentiation on food protein binding capacities of the brush border membrane. Cell morphology and expression of brush border-associated enzymes were studied as differentiation markers. Food protein binding to isolated brush border membranes was measured with a dot blot chemiluminescence assay. Early at confluence, Caco-2 cells exhibited a more differentiated state compared to T84 cells. Brush border membranes of both cell lines bound gliadin peptides, beta-lactoglobulin and ovalbumin specifically. Binding capacities increased from gliadin peptides to ovalbumin to beta-lactoglobulin. There was correlation of membrane binding capacity with degree of cell differentiation. Due to their similarity to small intestinal epithelial cells, the colon carcinoma cell lines Caco-2 and T84 represent models for studying food protein-enterocytic brush border membrane interactions in relation to varying degrees of cell differentiation.
Bolte
G
G
University Children's Hospital, Germany.
Wolburg
H
H
Beuermann
K
K
Stocker
S
S
Stern
M
M
D018938:Caco-2 Cells; D002454:Cell Differentiation; D002460:Cell Line; D003110:Colonic Neoplasms / Q000378:metabolism; D004044:Dietary Proteins / Q000378:metabolism*; D004847:Epithelial Cells / Q000378:metabolism; D005614:Freeze Fracturing; D005903:Gliadin / Q000378:metabolism; D006801:Humans; D007413:Intestinal Mucosa / Q000378:metabolism*; D007782:Lactoglobulins / Q000378:metabolism; D008163:Luminescent Measurements; D008854:Microscopy, Electron; D008871:Microvilli / Q000378:metabolism*; D010047:Ovalbumin / Q000378:metabolism
D004044:Dietary Proteins; D007782:Lactoglobulins; D010047:Ovalbumin; D005903:Gliadin