title: The effects of excretions/secretions of Ostertagia circumcincta on ovine abomasal tissues in vitro.
abstract: Products excreted/secreted by Ostertagia circumcincta stimulated the in vitro release of pepsinogen from intact abomasal mucosal sheets and caused the contraction of strips of abomasal smooth muscle, also in vitro. However, responses occurred only when tissues had been derived from animals that were assumed to have experienced prior exposure to the parasite. The overall median responses for pepsinogen secretion in response to ES, expressed as the ratio of stimulated secretion to basal secretion, were 1.8 for previously exposed animals and 0.9 for parasite-naive animals. For the smooth muscle from the previously exposed animals, the overall median response to ES, expressed as a percentage of the maximal response to carbachol in the same tissues, was 27.0. No responses were seen in muscle from any parasite-naive animal. These results suggest that the responses obtained were hypersensitivity reactions to antigens released by the worms during in vitro culture, and occurring in tissues from animals sensitised by exposure to O. circumcincta in the natural environment.
authors.affiliation: Department of Veterinary Pharmacology, University of Glasgow Veterinary School, U.K. I.Scott@massey.ac.nz
authors.forename: I
authors.initials: I
authors.lastname: Scott
authors.forename: Q A
authors.initials: QA
authors.lastname: McKellar
mesh_terms: D000018:Abomasum / Q000378:metabolism / Q000469:parasitology / Q000502:physiology*; D000818:Animals; D002217:Carbachol / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D005260:Female; D005753:Gastric Mucosa / Q000187:drug effects / Q000378:metabolism; D066298:In Vitro Techniques; D008297:Male; D009119:Muscle Contraction / Q000187:drug effects; D009130:Muscle, Smooth / Q000187:drug effects / Q000502:physiology; D010028:Ostertagia / Q000378:metabolism*; D010277:Parasympathomimetics / Q000494:pharmacology; D010435:Pepsinogens / Q000378:metabolism*; D012756:Sheep
chemical_list: D010277:Parasympathomimetics; D010435:Pepsinogens; D002217:Carbachol