title: Bacteria-induced neo-biosynthesis, stabilization, and surface expression of functional class I molecules in mouse dendritic cells.
abstract: Here, we show that bacteria induce de novo synthesis of both major histocompatability complex (MHC) class I and II molecules in a mouse dendritic cell culture system. The neo-biosynthesis of MHC class I molecules is delayed as compared with that of MHC class II. Furthermore, bacteria stabilize MHC class I molecules by a 3-fold increase of their half-life. This has important consequences for the capacity of dendritic cells to present bacterial antigens in the draining lymph nodes. In addition, a model antigen, ovalbumin, expressed on the surface of recombinant Streptococcus gordonii is processed and presented on MHC class I molecules. This presentation is 10(6) times more efficient than that of soluble OVA protein. This exogenous pathway of MHC class I presentation is transporter associated with antigen processing (TAP)-dependent, indicating that there is a transport from phagolysosome to cytosol in dendritic cells. Thus, bacteria are shown to be a potentially useful mean for the correct delivery of exogenous antigens to be presented efficiently on MHC class I molecules.
authors.affiliation: Consiglio Nazionale delle Ricerche, Centre of Cellular and Molecular Pharmacology, Via Vanvitelli 32, 20129 Milano, Italy.
authors.forename: M
authors.initials: M
authors.lastname: Rescigno
authors.forename: S
authors.initials: S
authors.lastname: Citterio
authors.forename: C
authors.initials: C
authors.lastname: Thèry
authors.forename: M
authors.initials: M
authors.lastname: Rittig
authors.forename: D
authors.initials: D
authors.lastname: Medaglini
authors.forename: G
authors.initials: G
authors.lastname: Pozzi
authors.forename: S
authors.initials: S
authors.lastname: Amigorena
authors.forename: P
authors.initials: P
authors.lastname: Ricciardi-Castagnoli
mesh_terms: D000071181:ATP Binding Cassette Transporter, Subfamily B, Member 2; D018528:ATP-Binding Cassette Transporters / Q000378:metabolism; D000818:Animals; D000938:Antigen-Presenting Cells / Q000276:immunology*; D000941:Antigens / Q000737:chemistry* / Q000276:immunology; D000954:Antigens, Surface / Q000276:immunology; D001419:Bacteria / Q000276:immunology*; D016207:Cytokines / Q000096:biosynthesis; D003713:Dendritic Cells / Q000276:immunology*; D004306:Dose-Response Relationship, Immunologic; D015395:Histocompatibility Antigens Class I / Q000096:biosynthesis*; D000949:Histocompatibility Antigens Class II / Q000276:immunology; D051379:Mice; D010047:Ovalbumin / Q000276:immunology; D010587:Phagocytosis; D011994:Recombinant Proteins; D013291:Streptococcus
chemical_list: D000071181:ATP Binding Cassette Transporter, Subfamily B, Member 2; D018528:ATP-Binding Cassette Transporters; D000941:Antigens; D000954:Antigens, Surface; D016207:Cytokines; D015395:Histocompatibility Antigens Class I; D000949:Histocompatibility Antigens Class II; D011994:Recombinant Proteins; C073119:TAP1 protein, human; C486171:Tap1 protein, mouse; D010047:Ovalbumin