Antioxidant defenses in lung lining fluid of broilers: impact of poor ventilation conditions.
Lung lining fluid antioxidants represent a potentially important protective barrier of lung epithelial cells to damaging effects of air pollutants, yet no information is apparently available concerning lung lining fluid antioxidants in broilers. Therefore, goals of this study were to establish uric acid, ascorbic acid, reduced (GSH) and oxidized (GSSG) glutathione, and protein concentrations in lung lining fluid obtained from male broiler chickens maintained for 6 to 7 wk within environmentally controlled rooms (Control) or chronically exposed to high levels of dust and ammonia within a broiler rearing house (House). The entire respiratory tract was carefully removed following an overdose of anesthetic and lavage fluid was collected after flushing the lungs with heparin-saline (10 mL per lung). There was no difference in GSH, but GSSG, uric acid, and protein concentrations were higher in House birds than in Controls. An increase in the GSSG to total glutathione (GSx) ratio, an indicator of oxidative stress, was also observed in birds maintained in the House environment. Ascorbic acid was not detected in House-reared birds and detected in only 4 of 12 Controls. Regression analysis revealed positive correlations between lung lining fluid protein and uric acid (r = 0.71; P < 0.01), protein and GSSG (r = 0.73; P < 0.01), and uric acid and GSSG concentrations (r = 0.69, P < 0.01). Additionally, GSSG was positively correlated (r = 0.66; P < 0.01) with the right ventricular weight ratio, an index commonly used in identifying the development of pulmonary hypertension syndrome in broilers. These data, the first to document lung lining fluid antioxidants in avian species, indicate an oxidative stress can be detected in fluid of broilers exposed to high levels of dust and ammonia in a simulated poultry house environment.
Bottje
W G
WG
Department of Poultry Science, Center of Excellence for Poultry Science, University of Arkansas, Fayetteville 72701, USA. wbottje@comp.uark.edu
Wang
S
S
Kelly
F J
FJ
Dunster
C
C
Williams
A
A
Mudway
I
I
D000818:Animals; D000975:Antioxidants / Q000032:analysis*; D001205:Ascorbic Acid / Q000032:analysis; D001992:Bronchoalveolar Lavage Fluid / Q000737:chemistry*; D002645:Chickens / Q000502:physiology*; D015331:Cohort Studies; D004032:Diet / Q000662:veterinary; D005978:Glutathione / Q000032:analysis / Q000737:chemistry; D019803:Glutathione Disulfide / Q000032:analysis; D006799:Housing, Animal / Q000592:standards; D008297:Male; D010084:Oxidation-Reduction; D018384:Oxidative Stress / Q000502:physiology*; D011506:Proteins / Q000032:analysis; D014527:Uric Acid / Q000032:analysis; D014691:Ventilation / Q000379:methods / Q000592:standards*
D000975:Antioxidants; D011506:Proteins; D014527:Uric Acid; D005978:Glutathione; D001205:Ascorbic Acid; D019803:Glutathione Disulfide