title: Directionality of polypeptide transfer in the mitochondrial pathway of chaperone-mediated protein folding.
abstract: Protein folding in mitochondria depends on the functional cooperation of the Hsp70 and Hsp60 chaperone systems, at least for a subset of mitochondrial polypeptides. As suggested previously, Hsp70 and Hsp60 act sequentially. However, recent proposals that the chaperonin Hsp60 functions by releasing substrate protein in an unfolded state would predict a lateral partitioning of folding intermediates between chaperone systems. Firefly luciferase, carrying a mitochondrial targeting signal, was used as a model protein to analyze the degree of coupling and the directionality of substrate transfer between the Hsp70 and Hsp60 chaperones. In vitro, Hsp60 binds unfolded luciferase with high affinity but is unable to promote its folding, whereas the Hsp70 system assists the folding of luciferase efficiently. Upon import into yeast mitochondria, luciferase interacted first with Hsp70. Surprisingly, most of the protein subsequently accumulated in a complex with Hsp60 and never reached the native state. Import into mitochondria that lack a functional Hsp60 did not result in increased folding, but in the aggregation of luciferase. Thus, in intact organelles the two chaperone systems do not function independently in de novo folding of aggregation-sensitive proteins but rather act in an ordered pathway with substrate transfer predominantly in the direction from Hsp70 to Hsp60.
authors.affiliation: Cellular Biochemistry and Biophysics Program and Howard Hughes Medical Institute, Memorial Sloan-Kettering Cancer Center, New York, NY 10021, USA.
authors.forename: N
authors.initials: N
authors.lastname: Heyrovská
authors.forename: J
authors.initials: J
authors.lastname: Frydman
authors.forename: J
authors.initials: J
authors.lastname: Höhfeld
authors.forename: F U
authors.initials: FU
authors.lastname: Hartl
mesh_terms: D001692:Biological Transport; D008156:Luciferases / Q000235:genetics / Q000378:metabolism; D008928:Mitochondria / Q000378:metabolism*; D008954:Models, Biological; D018832:Molecular Chaperones / Q000378:metabolism*; D009492:Neurospora crassa / Q000201:enzymology; D010455:Peptides / Q000378:metabolism*; D017510:Protein Folding; D006180:Proton-Translocating ATPases / Q000235:genetics / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000235:genetics
chemical_list: D018832:Molecular Chaperones; D010455:Peptides; D008156:Luciferases; D006180:Proton-Translocating ATPases