title: Platelets induce monocyte differentiation in serum-free coculture.
abstract: Terminal maturation of blood monocytes (MO) in vitro and in vivo into macrophages (MAC) occurs as a result of interactions with various cell types. To characterize some of the cell-cell connections that may be important for MO differentiation we cocultured human MO with lymphocytes and/or with platelets. We found that intact platelets strongly promoted MO maturation under serum-free conditions as evident from the expression of differentiation-dependent antigens and morphology. To further characterize the differentiation-inducing component(s) we prepared membrane and cytosol fractions of platelets. Both fractions could induce MO maturation, comparable to intact platelets. Further centrifugation of the cytosolic fraction revealed that only the pellet of ultracentrifugation, e.g., membrane fragments, could induce MO differentiation. Digestion with either trypsin or neuraminidase could only partially inhibit this effect. The same was true for heat-treated fractions, indicating that this platelet-derived differentiation stimulus is not solely an intact protein. Next we prepared protein and lipid fractions of platelets. Treatment of MO with platelet proteins or platelet lipids clearly showed that only the lipid components were able to induce MO maturation. We propose components present in the lipid fraction of platelet membranes as possible inducers of MO maturation in vitro.
authors.affiliation: Department of Hematology and Oncology, University of Regensburg, Germany.
authors.forename: C
authors.initials: C
authors.lastname: Ammon
authors.forename: M
authors.initials: M
authors.lastname: Kreutz
authors.forename: M
authors.initials: M
authors.lastname: Rehli
authors.forename: S W
authors.initials: SW
authors.lastname: Krause
authors.forename: R
authors.initials: R
authors.lastname: Andreesen
mesh_terms: D000906:Antibodies / Q000494:pharmacology; D001792:Blood Platelets / Q000502:physiology*; D002454:Cell Differentiation / Q000187:drug effects; D002478:Cells, Cultured; D018920:Coculture Techniques; D016895:Culture Media, Serum-Free; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D008214:Lymphocytes / Q000502:physiology; D008563:Membrane Lipids / Q000502:physiology; D008565:Membrane Proteins / Q000502:physiology; D009000:Monocytes / Q000502:physiology*; D010982:Platelet-Derived Growth Factor / Q000276:immunology / Q000494:pharmacology; D016212:Transforming Growth Factor beta / Q000276:immunology / Q000494:pharmacology
chemical_list: D000906:Antibodies; D016895:Culture Media, Serum-Free; D008563:Membrane Lipids; D008565:Membrane Proteins; D010982:Platelet-Derived Growth Factor; D016212:Transforming Growth Factor beta