title: Prominent occurrence of ribosomes from an uncultured bacterium of the Verrucomicrobiales cluster in grassland soils.
abstract: An uncultured bacterium of the Verrucomicrobiales cluster was identified by its 16S rDNA sequence as a major bacterium in Dutch Drentse A grassland soils. Potential metabolic activity of the according organism was estimated by applying direct ribosome isolation from soil and partial amplification of the 16S rRNA via RT-PCR using bacteria-specific primers. Temperature gradient gel electrophoresis separated the amplicons sequence specifically into reproducible fingerprints. One of the fingerprint bands matched with the signal of clone DA101. Southern blot hybridization with a DA101-specific V6 probe confirmed sequence identity. It is the first time that an organism of the Verrucomicrobiales cluster has been indicated as a potential major metabolizer in environmental microbial communities.
authors.affiliation: Department of Biomolecular Sciences, Wageningen Agricultural University, The Netherlands. Andreas.Felske@algemeen.micr.wau.nl
authors.forename: A
authors.initials: A
authors.lastname: Felske
authors.forename: A D
authors.initials: AD
authors.lastname: Akkermans
mesh_terms: D001419:Bacteria / Q000145:classification*; D004275:DNA, Ribosomal / Q000737:chemistry; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012270:Ribosomes / Q000737:chemistry*; D012988:Soil Microbiology
chemical_list: D004275:DNA, Ribosomal; D012336:RNA, Ribosomal, 16S