title: Identification and expression in mouse of two heparan sulfate glucosaminyl N-deacetylase/N-sulfotransferase genes.
abstract: The biosynthesis of heparan sulfate/heparin is a complex process that requires the coordinate action of a number of different enzymes. In close connection with polymerization of the polysaccharide chain, the modification reactions are initiated by N-deacetylation followed by N-sulfation of N-acetylglucosamine units. These two reactions are carried out by a single protein. Proteins with such dual activities were first purified and cloned from rat liver and mouse mastocytoma. The mouse mastocytoma enzyme is encoded by an approximately 4-kilobase (kb) mRNA, whereas the rat liver transcript contains approximately 8 kb. In the present study, the primary structure of the enzyme encoded by the mouse 8-kb transcript is described. It is demonstrated that both the 4-and 8-kb transcripts have a wide tissue distribution and that they are encoded by separate genes. Characterization of the gene encoding the 4-kb transcript demonstrates that it spans a region of about 8 kb and that it contains at least 14 exons. The similarity of this gene and the previously characterized human gene for the 8-kb transcript is discussed.
authors.affiliation: Department of Medical Biochemistry and Microbiology, University of Uppsala, S 751 23 Uppsala, Sweden. Marion.Kusche@medkem.uu.se
authors.forename: M
authors.initials: M
authors.lastname: Kusche-Gullberg
authors.forename: I
authors.initials: I
authors.lastname: Eriksson
authors.forename: D S
authors.initials: DS
authors.lastname: Pikas
authors.forename: L
authors.initials: L
authors.lastname: Kjellén
mesh_terms: D000581:Amidohydrolases / Q000235:genetics*; D000595:Amino Acid Sequence; D000818:Animals; D005091:Exons; D005796:Genes; D007438:Introns; D008099:Liver / Q000201:enzymology; D051379:Mice; D008969:Molecular Sequence Data; D012333:RNA, Messenger / Q000235:genetics; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid; D015238:Sulfotransferases / Q000235:genetics*; D014018:Tissue Distribution
chemical_list: D012333:RNA, Messenger; D015238:Sulfotransferases; C021350:heparitin sulfotransferase; D000581:Amidohydrolases