Chromosome aberrations in bone marrow cells of C3H/He mice at an early stage after whole-body irradiation.
Murine acute myeloid leukemia is characterized by chromosome 2 aberrations, and genesis of the marker chromosome 2 by radiation is suspected to be an initiating event of radiation leukemogenesis. A detailed analysis of the type and frequency of chromosome 2 aberrations in murine bone marrow cells at an early stage after irradiation is provided here. A total of 40 male C3H/He mice was exposed to 137Cs gamma-ray at a dose of 1, 2 or 3 Gy, and sacrificed 24 hours after irradiation. Metaphase samples prepared from bone marrow cells were Q-banded for karyotyping or painted with DNA probes specific to chromosome 2. In 5 mice analyzed by karyotyping, one mouse showed high frequency of the marker aberrations as well as other chromosome 2 aberrations. Chromosome painting analysis for the rest of the mice also detected 3 animals showing significantly high frequencies of chromosome 2 aberrations. Dose-dependence of the frequencies was observed even among those mice that tended to be sensitive. The results indicated that there was a subgroup of mice carrying hypersensitive chromosome 2. The subgroup could be leukemia-sensitive if radiation-induced chromosome aberrations are responsible for an early change in myeloid leukemogenesis.
Ban
N
N
Department of Radiological Health, Faculty of Medicine, University of Tokyo, Japan. ban@nile.gen.u-tokyo.ac.jp
Kai
M
M
Kusama
T
T
D000818:Animals; D001854:Bone Marrow Cells / Q000528:radiation effects*; D002869:Chromosome Aberrations; D007621:Karyotyping; D008297:Male; D051379:Mice; D008809:Mice, Inbred C3H; D014916:Whole-Body Irradiation