In adrenocortical tissue, annexins II and VI are attached to clathrin coated vesicles in a calcium-independent manner.
We have previously characterized three populations of clathrin coated vesicles (CCVs) isolated from bovine adrenocortical tissue and designated them as large, medium and small coated vesicles, i.e., LCV, MCV and SCV, respectively. Here, we show that annexins II and VI, two of the annexins involved in membrane traffic, are present in the three populations of CCVs but with different distributions between coat proteins (CP) and lipidic vesicle membrane. Annexin VI is only associated with the membrane, whatever the CCV population. In contrast, annexin II is differently distributed between coat and membrane, depending on the CCV population. Both annexins are bound to membranes in a calcium-independent manner and solubilization studies in Triton X114 (TX114) suggest that they interact poorly with lipids by hydrophobic interactions. Ligand blotting experiments show that both annexins bind to CCV proteins: annexin II to a 200-kDa component in all CCVs and annexin VI to a 100-kDa component in LCV and SCV identified as dynamin, a GTPase essential for endocytic CCV pinching off. Dynamin is tightly associated to annexin VI only in LCVs, the endocytic [transferrin (Tf) positive] vesicles. Our data suggest that annexins II and VI could define specific protein-lipid interaction microdomains that could play a role in the different functions of the CCVs.
Turpin
E
E
Etats Liés Moléculaires, Faculté de Médecine, Paris, France.
Russo-Marie
F
F
Dubois
T
T
de Paillerets
C
C
Alfsen
A
A
Bomsel
M
M
D000302:Adrenal Cortex / Q000737:chemistry*; D000818:Animals; D017306:Annexin A2 / Q000737:chemistry / Q000378:metabolism*; D017317:Annexin A6 / Q000737:chemistry / Q000276:immunology / Q000378:metabolism*; D019556:COS Cells / Q000378:metabolism; D002118:Calcium / Q000378:metabolism*; D002417:Cattle; D002966:Clathrin / Q000378:metabolism*; D018699:Coated Vesicles / Q000737:chemistry / Q000378:metabolism; D003902:Detergents / Q000737:chemistry; D034281:Dynamins; D020558:GTP Phosphohydrolases / Q000378:metabolism; D006367:HeLa Cells / Q000378:metabolism; D006801:Humans; D008051:Lipid Bilayers; D011233:Precipitin Tests; D011506:Proteins / Q000378:metabolism; D011817:Rabbits; D012995:Solubility
D017306:Annexin A2; D017317:Annexin A6; D002966:Clathrin; D003902:Detergents; D008051:Lipid Bilayers; D011506:Proteins; D020558:GTP Phosphohydrolases; D034281:Dynamins; D002118:Calcium