Cytologic and immunoenzymatic findings in CSF from patients with tick-borne encephalitis.
OBJECTIVE
To evaluate the dynamics of cytologic changes in the cerebrospinal fluid (CSF) of patients with tick-borne encephalitis (TBE) and to determine the various forms of mononuclear cells by immunocytochemical methods.


METHODS
To perform a cytologic analysis of 200 cells in stained sediment of CSF. To determine the population and subpopulation of mononuclear cells by using monoclonal antibodies.


RESULTS
Pleocytosis in CSF in patients with TBE during the first three days of illness was, on average, 570 cells per cubic millimeter with about 60% neutrophils. Mononuclear cells predominated from the fifth day of illness. Among lymphoid cells, 18% carried marker CD20 and 60% of them marker CD3. Among monocytoid cells, 66% carried marker CD32.


CONCLUSIONS
CSF cytology findings reflect the immunologic and inflammatory changes in the CNS during TBE.
Jeren
T
T
Dr. Fran Mihaljević University Hospital for Infectious Diseases, University of Zagreb, Croatia.
Vince
A
A
D000293:Adolescent; D000328:Adult; D000368:Aged; D015703:Antigens, CD / Q000032:analysis; D002648:Child; D002675:Child, Preschool; D004675:Encephalitis, Tick-Borne / Q000134:cerebrospinal fluid* / Q000276:immunology*; D005260:Female; D006801:Humans; D007124:Immunoenzyme Techniques; D007223:Infant; D008297:Male; D008875:Middle Aged
D015703:Antigens, CD