Intravitam diagnosis of human rabies by PCR using saliva and cerebrospinal fluid.
An optimized reverse transcription (RT)-PCR protocol for the intravitam detection of rabies virus genomic RNA was tested with clinical samples obtained from 28 patients suspected of having rabies, 9 of whom were confirmed to have had rabies by postmortem examination. RT-PCR using saliva combined with an immunofluorescence assay performed with skin biopsy samples allowed detection of rabies in the nine patients.
Crepin
P
P
Rabies Unit, Institut Pasteur, Paris, France.
Audry
L
L
Rotivel
Y
Y
Gacoin
A
A
Caroff
C
C
Bourhy
H
H
D002555:Cerebrospinal Fluid / Q000821:virology*; D006801:Humans; D016133:Polymerase Chain Reaction; D012367:RNA, Viral / Q000032:analysis*; D011818:Rabies / Q000175:diagnosis*; D011820:Rabies virus / Q000235:genetics*; D012463:Saliva / Q000821:virology*
D012367:RNA, Viral