Effect of temperature on hepatic and renal uptake of components from University of Wisconsin solution.
In rat liver and kidney preservation, hepatic and renal uptake of 3H-adenosine, 3H-glutathione, and 3H-raffinose from University of Wisconsin solution and diffusion to the interstitial space were measured. At 4 degrees C only 0.38+/-0.47% and 2+/-0.92% of the total 3H-adenosine remained in the kidney and in the liver, respectively, but at 37 degrees C the amount remaining was 1+/-1% and 12+/-3% (P<0.001). Hepatic and renal uptake of the impermeant 3H-raffinose was unaffected by temperature. During flush out, interstitial accumulation of adenosine was significantly higher in livers than in kidneys and decreased during 24-h cold storage. Glutathione accumulation in the interstitial space was two orders of magnitude lower than 3H-adenosine accumulation and comparable to the impermeant raffinose. In summary, the bioavailability of components of preservation solutions at 4 degrees C is lower than at physiological temperatures, so that the application of cytoprotectants at 37 degrees C to organ donors, rather than simple addition to the cold storage solution, might improve cold storage preservation of livers and kidneys.
Schilling
M
M
Department of Visceral and Transplant Surgery, University of Bern, Inselspital, Switzerland.
Tian
Y H
YH
Büchler
M W
MW
D000241:Adenosine / Q000493:pharmacokinetics; D000493:Allopurinol / Q000493:pharmacokinetics; D000818:Animals; D005978:Glutathione / Q000493:pharmacokinetics; D007328:Insulin / Q000493:pharmacokinetics; D007668:Kidney; D008099:Liver; D008297:Male; D017551:Microdialysis; D009926:Organ Preservation / Q000379:methods*; D019188:Organ Preservation Solutions / Q000493:pharmacokinetics*; D010477:Perfusion; D011887:Raffinose / Q000493:pharmacokinetics; D051381:Rats; D017208:Rats, Wistar; D013696:Temperature; D014316:Tritium
D007328:Insulin; D019188:Organ Preservation Solutions; C055206:University of Wisconsin-lactobionate solution; D014316:Tritium; D000493:Allopurinol; D005978:Glutathione; D000241:Adenosine; D011887:Raffinose