title: Near-field fluorescence microscopy of cells.
abstract: We demonstrate the surface sensitivity of near-field scanning optical microscopy by fluorescence imaging of membrane and bulk proteins in cells. We discuss instrument design considerations for successful cell-biology work with NSOM and show that the technique is most suited for studying membrane proteins.
authors.affiliation: Molecular Design Institute, Materials Sciences Division, Lawrence Berkeley National Laboratory, Berkeley, CA 94720, USA.
authors.forename: T
authors.initials: T
authors.lastname: Enderle
authors.forename: T
authors.initials: T
authors.lastname: Ha
authors.forename: D S
authors.initials: DS
authors.lastname: Chemla
authors.forename: S
authors.initials: S
authors.lastname: Weiss
mesh_terms: D000818:Animals; D001412:Bacillus subtilis / Q000737:chemistry; D001426:Bacterial Proteins / Q000032:analysis*; D004910:Erythrocyte Membrane / Q000737:chemistry* / Q000469:parasitology / Q000648:ultrastructure; D049452:Green Fluorescent Proteins; D007202:Indicators and Reagents; D008164:Luminescent Proteins; D008565:Membrane Proteins / Q000032:analysis*; D018613:Microscopy, Confocal; D008855:Microscopy, Electron, Scanning; D008856:Microscopy, Fluorescence / Q000295:instrumentation* / Q000379:methods*; D055095:Optics and Photonics; D010963:Plasmodium falciparum; D015800:Protozoan Proteins / Q000032:analysis*; D012680:Sensitivity and Specificity; D013171:Spores, Bacterial / Q000737:chemistry / Q000648:ultrastructure
chemical_list: D001426:Bacterial Proteins; D007202:Indicators and Reagents; D008164:Luminescent Proteins; D008565:Membrane Proteins; D015800:Protozoan Proteins; D049452:Green Fluorescent Proteins