Predictive modelling of inactivation of Listeria spp. in bovine milk during high-temperature short-time pasteurization.
A linear model was derived to describe the thermal inactivation of Listeria innocua in bovine whole milk in a high-temperature short-time pilot scale pasteurizer. Integrated lethal effect, or pasteurization effect (PE), was obtained by converting times at different temperatures in the various sections of the pasteurizer to the equivalent time at the reference temperature (72 degrees C). PE was then related by a simple linear function to the log10 of the % viable counts with a power transformation of the PE values to improve the linear fit. R2 values for the five L. innocua trials varied from 0.728 to 0.974. Validation of this model with Listeria monocytogenes confirmed that L. monocytogenes was more heat sensitive. Inter-trial variation was incorporated into the model using the @RISK simulation software. Output from simulations confirmed that pasteurization at the IDF standard conditions of 72 degrees C for 15 sec can ensure at least an 11-log reduction of L. monocytogenes. The results showed that L. innocua may be used as a model microorganism to assess the thermal inactivation of L. monocytogenes, since its heat resistance is at least equal to or greater than that of the pathogenic species.
Piyasena
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Centre for Food and Animal Research, Agriculture and Agri-Food Canada, Ottawa, ON, Canada.
Liou
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McKellar
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RC
D000818:Animals; D002417:Cattle; D015169:Colony Count, Microbial; D003198:Computer Simulation; D005260:Female; D005525:Food-Processing Industry; D005544:Forecasting; D006358:Hot Temperature; D016014:Linear Models; D008089:Listeria monocytogenes / Q000254:growth & development*; D008892:Milk / Q000382:microbiology*; D008954:Models, Biological; D010865:Pilot Projects; D011336:Probability; D018570:Risk Assessment; D012986:Software Validation; D013242:Sterilization / Q000379:methods*; D013997:Time Factors