title: Differentiation of Cryptosporidium parvum isolates by a simplified randomly amplified polymorphic DNA technique.
abstract: Genomic DNA was isolated from Cryptosporidium parvum oocysts by a specific immunomagnetic separation-in vitro excystation procedure and subjected to randomly amplified polymorphic DNA analysis using sequence-independent primers. An estuary C. parvum isolate was easily differentiated from several bovine isolates, while five bovine isolates of the same origin were indistinguishable from each other.
authors.affiliation: Department of Population Health and Reproduction, School of Veterinary Medicine, University of California-Davis 95616, USA.
authors.forename: M Q
authors.initials: MQ
authors.lastname: Deng
authors.forename: D O
authors.initials: DO
authors.lastname: Cliver
mesh_terms: D000818:Animals; D002417:Cattle; D016785:Cryptosporidium parvum / Q000235:genetics*; D016054:DNA, Protozoan / Q000032:analysis*; D016133:Polymerase Chain Reaction; D019105:Random Amplified Polymorphic DNA Technique
chemical_list: D016054:DNA, Protozoan