Molecular cloning of leukotactin-1: a novel human beta-chemokine, a chemoattractant for neutrophils, monocytes, and lymphocytes, and a potent agonist at CC chemokine receptors 1 and 3.
A new member of human beta-chemokine cDNA was isolated and named leukotactin-1 (Lkn-1). Lkn-1, along with murine macrophage inflammatory protein-related protein-1 and -2, defines a subgroup of beta-chemokines based on two conserved cysteines in addition to the four others conserved in all beta-chemokines. The putative mature Lkn-1 is composed of 92 amino acids with a calculated m.w. of 10,162. The Lkn-1 gene was mapped to human chromosome 17, region q12. Recombinant Lkn-1 was a potent chemoattractant for neutrophils, monocytes, and lymphocytes and induced calcium flux in these cells. Lkn-1 specifically induced calcium flux in CCR1- and CCR3-expressing HOS cell lines. Lkn-1 suppressed colony formation by human granulocyte-macrophage, erythroid, and multipotential progenitor cells stimulated by combinations of growth factors. Hence, we have isolated and characterized a human C6 beta-chemokine that is a potent agonist at CCR1 and CCR3 and shows broad biologic activities, including leukocyte chemoattraction.
Youn
B S
BS
Department of Microbiology and Immunology, Indiana University School of Medicine, Indianapolis 46202, USA.
Zhang
S M
SM
Lee
E K
EK
Park
D H
DH
Broxmeyer
H E
HE
Murphy
P M
PM
Locati
M
M
Pease
J E
JE
Kim
K K
KK
Antol
K
K
Kwon
B S
BS
D000595:Amino Acid Sequence; D001483:Base Sequence; D002118:Calcium / Q000378:metabolism; D019742:Chemokines, CC / Q000235:genetics* / Q000276:immunology; D002633:Chemotaxis; D002874:Chromosome Mapping; D002886:Chromosomes, Human, Pair 17; D003001:Cloning, Molecular; D006801:Humans; D007962:Leukocytes / Q000276:immunology*; D008214:Lymphocytes / Q000276:immunology / Q000378:metabolism; D008969:Molecular Sequence Data; D009000:Monocytes / Q000276:immunology / Q000378:metabolism; D009504:Neutrophils / Q000276:immunology / Q000378:metabolism; D019707:Receptors, Chemokine / Q000037:antagonists & inhibitors*; D011994:Recombinant Proteins / Q000235:genetics; D013997:Time Factors
D019742:Chemokines, CC; D019707:Receptors, Chemokine; D011994:Recombinant Proteins; D002118:Calcium