title: Glucose interference in the pancreolauryl test.
abstract: The influence of the glucose concentration in urines being assayed as part of a pancreolauryl test was investigated. Paired patient urines (n = 5) were assayed at 60 degrees and 70 degrees C, before and after spiking to 5% glucose. The influence of assay temperature alone was assessed using glucose-free paired patient urines (n = 10). Aqueous glucose solutions and spiked (5% glucose) normal random urines (n = 5) were assayed to assess the effect of glucose concentration alone. There was no difference in T/K ratios for glucose-free patient samples at 60 degrees, 70 degrees and 80 degrees C. After spiking with glucose T/K ratios were significantly different when assayed at 70 degrees C, but not when assayed at 60 degrees C. Aqueous glucose solutions > or = 0.6% produced a pigment at temperatures > or = 65 degrees C which absorbed at the lambda max of fluorescein. Glucose was found to interfere in the pancreolauryl test. Caution should be exercised when interpreting results from glucosuric samples.
authors.affiliation: Department of Chemical Pathology, University College London Hospitals, UK.
authors.forename: A L
authors.initials: AL
authors.lastname: Trewick
mesh_terms: D005452:Fluoresceins; D005947:Glucose; D006801:Humans; D006868:Hydrolysis; D010186:Pancreatic Function Tests / Q000379:methods*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013696:Temperature
chemical_list: D005452:Fluoresceins; C034833:fluorescein dilaurate; D005947:Glucose