title: Evidentiation of paramyosin (Sm-97) as a modulating antigen on granulomatous hypersensitivity to Schistosoma mansoni eggs.
abstract: A Schistosoma mansoni adult worm anionic fraction (PIII) has previously been shown to protect mice against challenge infection and to reduce pulmonary and hepatic granulomatous hypersensitivity. Serum from PIII-immunized rabbit was used to screen a lambda gt11 cDNA library from S. mansoni adult worm in order to identify antigens capable of modulating granulomatous hypersensitivity. We obtained four clones with 400 (Sm-III.11), 900 (Sm-III.16), 1100 (Sm-III.10) and 1300 (Sm-III.12) bp of length. All clone-specific antibodies were able to recognize most of the PIII components. The sequence analysis showed that these clones presented high homology with S. mansoni paramyosin (Sm-97). These findings ascribe a new function to this antigen with an important role in modulation of granulomatous hypersensitivity to S. mansoni eggs.
authors.affiliation: Departamento de Bioquímica e Imunologia, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Belo Horizonte, Brasil.
authors.forename: C
authors.initials: C
authors.lastname: Hirsch
authors.forename: C
authors.initials: C
authors.lastname: Carvalho-Queiroz
authors.forename: G R
authors.initials: GR
authors.lastname: Franco
authors.forename: S D
authors.initials: SD
authors.lastname: Pena
authors.forename: A J
authors.initials: AJ
authors.lastname: Simpson
authors.forename: A M
authors.initials: AM
authors.lastname: Goes
mesh_terms: D000818:Animals; D006099:Granuloma / Q000469:parasitology*; D010270:Parasite Egg Count; D011817:Rabbits; D012550:Schistosoma mansoni / Q000276:immunology*; D014335:Tropomyosin / Q000276:immunology*
chemical_list: D014335:Tropomyosin