Histidine utilisation operon (hut) is upregulated at low temperature in the antarctic psychrotrophic bacterium Pseudomonas syringae.
The antarctic psychrotrophic bacterium Pseudomonas syringae was mutagenised using a transposon Tn5-OT182 which facilitates identification of promoter fusions expressing the reporter gene (lacZ) for beta-galactosidase. Most mutants expressed beta-galactosidase both at optimal growth temperature (20-22 degrees C) and at low temperature (4 degrees C). But a small percentage of the mutants (approximately 5%) were unique in that they expressed beta-galactosidase activity predominantly at low temperature. One such mutant was found to have an insertion in the gene for urocanase (hutU) of the histidine utilisation (hut) operon. Direct assay of urocanase and histidase activity in wild-type cells of various antarctic psychrotrophic strains including P. syringae, P. fluorescens and P. putida also suggested that the hut operon is expressed at an elevated level at low temperature.
Kannan
K
K
Centre for Cellular and Molecular Biology, Hyderabad, India.
Janiyani
K L
KL
Shivaji
S
S
Ray
M K
MK
D000595:Amino Acid Sequence; D001483:Base Sequence; D004251:DNA Transposable Elements; D006639:Histidine / Q000378:metabolism*; D006638:Histidine Ammonia-Lyase / Q000235:genetics; D007763:Lac Operon; D008969:Molecular Sequence Data; D009876:Operon; D011549:Pseudomonas / Q000235:genetics*; D013696:Temperature; D015854:Up-Regulation
D004251:DNA Transposable Elements; D006639:Histidine; D006638:Histidine Ammonia-Lyase