title: Association between in vivo iododeoxyuridine labeling, MIB-1 expression, malignancy grade and clinical stage in human prostate cancer.
abstract: Large variability in the biological behavior of prostate cancer makes prognostic markers important. The extent of tumor cell proliferation has been suggested as an important predictor of clinical outcome. Fifty-five patients suspected of having or with previously diagnosed prostate cancer were labeled in vivo with IdUrd (a thymidine analogue incorporated into DNA in S-phase cells) by intravenous infusion before transurethral resection. IdUrd-labeled cells and MIB-1-positive cells were detected by immunohistochemistry. We found statistically significant associations between the tumor cell proliferation rates measured by in vivo IdUrd labeling and MIB-1 expression in formalin-fixed paraffin-embedded tumors. Good correlations were also found between S-phase fraction, MIB-1 expression, clinical stage and malignancy grade. These results make larger retrospective studies on archival tissue meaningful.
authors.affiliation: Danish Cancer Society, Department of Experimental Clinical Oncology, University Hospital of Aarhus, Denmark.
authors.forename: M
authors.initials: M
authors.lastname: Borre
authors.forename: M
authors.initials: M
authors.lastname: Høyer
authors.forename: F B
authors.initials: FB
authors.lastname: Sørensen
authors.forename: B
authors.initials: B
authors.lastname: Nerstrøm
authors.forename: J
authors.initials: J
authors.lastname: Overgaard
mesh_terms: D000368:Aged; D000369:Aged, 80 and over; D034961:Antigens, Nuclear; D002455:Cell Division; D006801:Humans; D007065:Idoxuridine / Q000378:metabolism*; D019394:Ki-67 Antigen / Q000032:analysis; D008297:Male; D008875:Middle Aged; D009367:Neoplasm Staging; D009687:Nuclear Proteins / Q000032:analysis*; D011379:Prognosis; D011471:Prostatic Neoplasms / Q000473:pathology*
chemical_list: D034961:Antigens, Nuclear; D019394:Ki-67 Antigen; D009687:Nuclear Proteins; D007065:Idoxuridine