A barbiturate screening assay for the Abbott AxSYM analyzer.
A fluorescence polarization immunoassay for barbiturates on the Abbott AxSYM analyzer is described. The assay displayed dilution linearity up to 1200 ng/mL; coefficients of variation varied from 5.96 to 8.61%; recovery varied from 94.9 to 105.3%; and sensitivity was less than 40 ng/mL. Good correlation between the standard six- and factory two-point calibration methods was observed. The Immunoassay demonstrated good cross-reactivity to several commonly prescribed barbiturates; low cross-reactivity with structurally similar compounds; low interference from endogenous substances, dyes, preservatives, and several commonly available adulterants; and good correlation with the TDx Barbiturate Urine assay.
Adamczyk
M
M
Department of Chemistry, Abbott Laboratories, Abbott Park, Illinois 60064-3500, USA.
Douglas
J
J
Grote
J
J
Harrington
C A
CA
D001322:Autoanalysis / Q000295:instrumentation; D004340:Drug Contamination; D004353:Drug Evaluation, Preclinical; D016231:Fluorescence Polarization Immunoassay / Q000379:methods; D008401:Gas Chromatography-Mass Spectrometry; D006801:Humans; D006993:Hypnotics and Sedatives / Q000032:analysis* / Q000276:immunology / Q000652:urine; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D015813:Substance Abuse Detection / Q000379:methods*
D006993:Hypnotics and Sedatives