Effect of alpha-tocopherol status on alpha-tocopherol transfer protein expression and its messenger RNA level in rat liver.
Studies were designed to explore the role of vitamin E nutritional status in regulating the synthesis of alpha-tocopherol transfer protein (alphaTTP) in rat liver. In vitamin E-replete rats, expression of this protein and its specific messenger RNA (mRNA) was decreased by about 300% compared with rats with normal vitamin E levels. In vitamin E-depleted rats, the alphaTTP-specific mRNA level increased to about 150% of that in the normal vitamin E group. However, protein expression remained constant. These findings indicate that the synthesis of alphaTTP can be regulated by the vitamin E nutritional status.
Kim
H S
HS
Department of Pediatrics, Osaka Medical College, Takatsuki, Japan.
Arai
H
H
Arita
M
M
Sato
Y
Y
Ogihara
T
T
Inoue
K
K
Mino
M
M
Tamai
H
H
D000818:Animals; D015152:Blotting, Northern; D015153:Blotting, Western; D002352:Carrier Proteins / Q000235:genetics*; D015870:Gene Expression; D008099:Liver / Q000378:metabolism*; D008297:Male; D009752:Nutritional Status; D012333:RNA, Messenger / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley; D014810:Vitamin E / Q000008:administration & dosage / Q000097:blood* / Q000378:metabolism
D002352:Carrier Proteins; D012333:RNA, Messenger; C017763:alpha-tocopherol transfer protein; D014810:Vitamin E