title: Interactions of primary amphipathic vector peptides with membranes. Conformational consequences and influence on cellular localization.
abstract: The conformations of two peptides produced by the combinations of a nuclear localization sequence and a sequence issued from the fusion protein gp41 of HIV 1 have been analyzed both in solution and in membranes or in membrane mimicking environments. Both are shown to be nonordered in water, alpha-helical when incorporated into SDS micelles where the helical domain concerns the hydrophobic part of the peptides. Interactions with lipids induce the formation of beta-sheet and the lipid-peptide interactions are governed by the nature of the lipid polar headgroups. A monolayer study shows that replacement of the sequence separating the two sequences with an arginine favors the lipid-peptide interactions which may contribute to the understanding of the different, nuclear and membrane associated, cellular localizations of the peptides.
authors.affiliation: CRBM-CNRS (UPR 1086), 1919, Route de Mende, F. 34293 Montpellier Cedex 5, France.
authors.forename: P
authors.initials: P
authors.lastname: Vidal
authors.forename: L
authors.initials: L
authors.lastname: Chaloin
authors.forename: A
authors.initials: A
authors.lastname: Heitz
authors.forename: N
authors.initials: N
authors.lastname: Van Mau
authors.forename: J
authors.initials: J
authors.lastname: Méry
authors.forename: G
authors.initials: G
authors.lastname: Divita
authors.forename: F
authors.initials: F
authors.lastname: Heitz
mesh_terms: D000595:Amino Acid Sequence; D015700:HIV Envelope Protein gp41 / Q000737:chemistry* / Q000378:metabolism; D006801:Humans; D008567:Membranes, Artificial; D008969:Molecular Sequence Data; D010455:Peptides / Q000737:chemistry* / Q000378:metabolism; D010743:Phospholipids / Q000378:metabolism; D011487:Protein Conformation; D013057:Spectrum Analysis; D014867:Water
chemical_list: D015700:HIV Envelope Protein gp41; D008567:Membranes, Artificial; D010455:Peptides; D010743:Phospholipids; D014867:Water