Replication of human immunodeficiency virus type 1 (HIV-1), simian immunodeficiency virus strain mac (SIVmac) and chimeric HIV-1/SIVmac viruses having env genes derived from macrophage-tropic viruses: an indication of different mechanisms of macrophage-tropism in human and monkey cells.
To investigate the transferability of macrophage (Mphi)-tropism among primate lentiviruses, we constructed recombinants of human immunodeficiency virus type 1 (HIV-1), simian immunodeficiency virus strain mac (SIVmac) and chimeric HIV-1/SIVmac (SHIV) having env region Mphi-tropic determinants. A recombinant HIV-1 having env partially derived from a Mphi-tropic HIV-1 strain (JR-FL) replicated in human macrophages but not in monkey macrophages. Conversely, a recombinant SIVmac having env from a Mphi-tropic strain (SIVmac316) replicated in monkey macrophages but not in human macrophages. A new SHIV (designated NM-3rN/JRFL) carrying the LTR and gag, pol, vif, vpx and nef of SIVmac and vpr, tat, rev, vpu and env of HIV-1 with env partially replaced by that of JR-FL was replication-competent in human macrophages but not in monkey macrophages. These results suggest that the Mphi-tropic determinant is specific to each host species and that the mechanism of Mphi-tropism is different between HIV and SIV.
Chen
J
J
Laboratory of Pathogenic Virus, Institute for Virus Research, Kyoto University, Japan.
Ido
E
E
Jin
M
M
Kuwata
T
T
Igarashi
T
T
Mizuno
A
A
Koyanagi
Y
Y
Hayami
M
M
D000818:Animals; D002460:Cell Line; D002678:Chimera / Q000235:genetics / Q000502:physiology; D005814:Genes, Viral; D015752:Genes, env; D015497:HIV-1 / Q000235:genetics* / Q000472:pathogenicity / Q000502:physiology*; D000882:Haplorhini; D006801:Humans; D008264:Macrophages / Q000821:virology; D015302:Simian Immunodeficiency Virus / Q000235:genetics* / Q000472:pathogenicity / Q000502:physiology*; D013045:Species Specificity; D014774:Virulence / Q000235:genetics; D014779:Virus Replication / Q000235:genetics