Oncostatin M stimulates the expression and release of the IL-6 receptor in human hepatoma HepG2 cells.
Soluble IL-6R (sIL-6R), which lacks the transmembrane domain, has been suggested to be a potent immunomodulator of IL-6 biologic activity. In this study, the ability of cells of hepatic origin to generate the sIL-6R was investigated. It was found that oncostatin M alone or in combination with the glucocorticoid analogue dexamethasone significantly up-regulated IL-6R release. Oncostatin M appeared to generate the sIL-6R primarily through an alternative splicing mechanism. Since sIL-6R is able to form biologically active complexes with IL-6, the release of the sIL-6R from hepatocytes may be important to sensitize cells lacking the membrane-bound receptor, particularly during an acute phase reaction.
Cichy
J
J
Department of Microbiology and Immunology, Institute of Molecular Biology, Jagiellonian University, Krakow, Poland.
Rose-John
S
S
Potempa
J
J
Pryjma
J
J
Travis
J
J
D017398:Alternative Splicing; D000970:Antineoplastic Agents / Q000494:pharmacology; D006528:Carcinoma, Hepatocellular / Q000378:metabolism*; D003907:Dexamethasone / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D006131:Growth Inhibitors / Q000494:pharmacology; D006801:Humans; D053683:Oncostatin M; D010455:Peptides / Q000494:pharmacology*; D010618:Phenanthrolines / Q000494:pharmacology; D019947:Receptors, Interleukin-6 / Q000378:metabolism*; D014162:Transfection; D014407:Tumor Cells, Cultured
D000970:Antineoplastic Agents; D006131:Growth Inhibitors; C508351:OSM protein, human; D010455:Peptides; D010618:Phenanthrolines; D019947:Receptors, Interleukin-6; D053683:Oncostatin M; D003907:Dexamethasone; C025205:1,10-phenanthroline