title: hUBC9 associates with MEKK1 and type I TNF-alpha receptor and stimulates NFkappaB activity.
abstract: hUBC9, an E2 ubiquitin conjugating enzyme, was identified by yeast two-hybrid screening and coprecipitation studies to interact with MEKK1 and the type I TNF-alpha receptor, respectively. Because both of these proteins regulate NFkappaB activity, the role of hUBC9 in modulating NFkappaB activity was investigated. Overexpression of hUBC9 in HeLa cells stimulated the activity of NFkappaB as determined by NFkappaB reporter and IL-6 secretion assays. hUBC9 also synergized with MEKK1 to activate NFkappaB reporter activity. Thus, hUBC9 modulates NFkappaB activity which, at least in part, can be attributed to its interaction with MEKK1 and the type I TNF-alpha receptor.
authors.affiliation: Rhône-Poulenc Rorer Central Research, Gene Medicine Department, Collegeville, PA 19426, USA.
authors.forename: A
authors.initials: A
authors.lastname: Saltzman
authors.forename: G
authors.initials: G
authors.lastname: Searfoss
authors.forename: C
authors.initials: C
authors.lastname: Marcireau
authors.forename: M
authors.initials: M
authors.lastname: Stone
authors.forename: R
authors.initials: R
authors.lastname: Ressner
authors.forename: R
authors.initials: R
authors.lastname: Munro
authors.forename: C
authors.initials: C
authors.lastname: Franks
authors.forename: J
authors.initials: J
authors.lastname: D'Alonzo
authors.forename: B
authors.initials: B
authors.lastname: Tocque
authors.forename: M
authors.initials: M
authors.lastname: Jaye
authors.forename: Y
authors.initials: Y
authors.lastname: Ivashchenko
mesh_terms: D015703:Antigens, CD / Q000378:metabolism*; D005786:Gene Expression Regulation / Q000235:genetics; D017930:Genes, Reporter / Q000235:genetics; D006367:HeLa Cells; D006801:Humans; D015850:Interleukin-6 / Q000378:metabolism; D008025:Ligases / Q000235:genetics / Q000378:metabolism*; D048728:MAP Kinase Kinase Kinase 1; D016296:Mutagenesis; D016328:NF-kappa B / Q000378:metabolism*; D017346:Protein Serine-Threonine Kinases / Q000378:metabolism*; D011505:Protein-Tyrosine Kinases / Q000378:metabolism*; D018124:Receptors, Tumor Necrosis Factor / Q000378:metabolism*; D047888:Receptors, Tumor Necrosis Factor, Type I; D011993:Recombinant Fusion Proteins / Q000378:metabolism; D014162:Transfection / Q000235:genetics; D044763:Ubiquitin-Conjugating Enzymes; D000098843:Ubiquitin-Conjugating Enzyme UBC9
chemical_list: D015703:Antigens, CD; D015850:Interleukin-6; D016328:NF-kappa B; D018124:Receptors, Tumor Necrosis Factor; D047888:Receptors, Tumor Necrosis Factor, Type I; D011993:Recombinant Fusion Proteins; D044763:Ubiquitin-Conjugating Enzymes; D011505:Protein-Tyrosine Kinases; D017346:Protein Serine-Threonine Kinases; D048728:MAP Kinase Kinase Kinase 1; C482576:MAP3K1 protein, human; D008025:Ligases; D000098843:Ubiquitin-Conjugating Enzyme UBC9