Determination of nimesulide and hydroxynimesulide in human plasma by high performance liquid chromatography.
Two specific methods for the simultaneous determination of nimesulide, a non steroidal anti-inflammatory drug, and its hydroxylated metabolite in human plasma are described. Adopting a high performance liquid chromatographic (HPLC) system with UV detection (230 nm), the compounds, extracted from plasma in acidic medium, were separated on ODS columns under gradient conditions, using a phosphate buffer solution and methanol as mobile phase. For each method column length, gradient rate and composition were appropriately selected. The limit of quantitation was 25 ng/mL for both compounds. The two methods were validated by intra day assays at three concentration levels and applied in kinetic studies in healthy volunteers, during which inter-day assays were carried out confirming their feasibility.
Giachetti
C
C
Istituto di Ricerche Biomediche A. Marxer, RBM, S.p.A., Ivrea, To, Italy.
Tenconi
A
A
D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000097:blood*; D016477:Artifacts; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D006801:Humans; D012015:Reference Standards; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013056:Spectrophotometry, Ultraviolet; D013449:Sulfonamides / Q000097:blood*
D000894:Anti-Inflammatory Agents, Non-Steroidal; D013449:Sulfonamides; C055891:4-nitro-2-(4'-hydroxyphenoxy)methanesulfonanilide; C012655:nimesulide