title: Isolation and properties of a novel phospholipase A from rat brain that hydrolyses fatty acids at sn-1 and sn-2 positions.
abstract: A Ca(2+)-independent phospholipase A that releases various fatty acids from sn-1 and sn-2 positions was partially purified from rat brain soluble fraction. The enzyme showed an approximate molecular mass of 300 kDa on gel filtration column chromatography. Its enzymatic properties are distinct from those of well characterized phospholipase A2 enzymes; by using a series of synthetic phosphatidylcholines, the enzyme cleaved oleic, linoleic, and arachidonic acids like phospholipase A2, and released palmitic and stearic acids like phospholipase A1. Phosphatidylcholine, phosphatidylethanolamine, phosphatidylinositol, and phosphatidic acid were hydrolysed with almost equal efficiencies by this enzyme. These results indicate that the enzyme isolated is a novel Ca(2+)-independent intracellular phospholipase A that might be responsible for production of various fatty acids from membrane phospholipids.
authors.affiliation: Department of Biochemistry, Kobe University School of Medicine, Japan.
authors.forename: H
authors.initials: H
authors.lastname: Yoshida
authors.forename: Y
authors.initials: Y
authors.lastname: Tsujishita
authors.forename: F
authors.initials: F
authors.lastname: Hullin
authors.forename: K
authors.initials: K
authors.lastname: Yoshida
authors.forename: S
authors.initials: S
authors.lastname: Nakamura
authors.forename: U
authors.initials: U
authors.lastname: Kikkawa
authors.forename: Y
authors.initials: Y
authors.lastname: Asaoka
mesh_terms: D000818:Animals; D001921:Brain / Q000201:enzymology*; D005227:Fatty Acids / Q000032:analysis / Q000737:chemistry / Q000378:metabolism*; D006868:Hydrolysis; D008297:Male; D010713:Phosphatidylcholines / Q000378:metabolism; D010741:Phospholipases A / Q000302:isolation & purification* / Q000378:metabolism*; D054466:Phospholipases A1; D054467:Phospholipases A2; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D005227:Fatty Acids; D010713:Phosphatidylcholines; D010741:Phospholipases A; D054466:Phospholipases A1; D054467:Phospholipases A2; C028694:1-palmitoyl-2-oleoylphosphatidylcholine