title: Construction of a 1.5-Mb bacterial artificial chromosome contig in Xp11.23, a region of high gene content.
abstract: To generate sequence-ready templates for the gene-rich Xp11.23 region, we have constructed a 1.5-Mb bacterial artificial chromosome (BAC) contig spanning the interval between the DNA markers OATL1 and DXS255. The contig includes 28 BACs, ranging in size from 58 to 258 kb with an average size of 135 kb, which provide 2.5-fold coverage of the region. The BAC contig was constructed based entirely on the content of 40 DNA markers from a previously established YAC contig and 11 new markers developed from BAC-end DNA sequences, 4 of which were required to close gaps in the map. There was no evidence of rearrangement, instability, or chimerism in any of the BAC clones. The BAC cloning system appears to provide robust and total physical coverage of this gene-rich region with clones that are suitable for DNA sequencing.
authors.affiliation: Department of Medical Genetics, Faculty of Medicine, University of Calgary, Alberta, Canada.
authors.forename: K M
authors.initials: KM
authors.lastname: Boycott
authors.forename: R J
authors.initials: RJ
authors.lastname: Zahorchak
authors.forename: C G
authors.initials: CG
authors.lastname: Summer
authors.forename: N P
authors.initials: NP
authors.lastname: Boycott
authors.forename: V
authors.initials: V
authors.lastname: Kotak
authors.forename: C G
authors.initials: CG
authors.lastname: Russell
authors.forename: N T
authors.initials: NT
authors.lastname: Bech-Hansen
mesh_terms: D002874:Chromosome Mapping; D002876:Chromosomes, Bacterial; D003001:Cloning, Molecular; D005819:Genetic Markers; D006801:Humans; D016133:Polymerase Chain Reaction; D012091:Repetitive Sequences, Nucleic Acid; D017422:Sequence Analysis, DNA; D016324:Sequence Tagged Sites; D014960:X Chromosome
chemical_list: D005819:Genetic Markers