title: Isolation and differential secretion of metalloproteinase by superficial chondrocytes in articular cartilage.
abstract: Chondrocytes from superficial layers of articular cartilage have distinct phenotypic properties which are different from those of cells obtained from the deeper areas. We describe a method that isolates highly purified articular cartilage chondrocytes from the superficial layers. When the superficial cells are stimulated in vitro with a source of cytokines, they secrete greater amounts of metalloproteinase compared to chondrocytes obtained from a deeper area.
authors.affiliation: Division of Rheumatology, University of Arkansas for Medical Sciences, Veterans Administration Hospital, Little Rock, USA.
authors.forename: T
authors.initials: T
authors.lastname: Takagi
authors.forename: S
authors.initials: S
authors.lastname: Takahashi
authors.forename: T
authors.initials: T
authors.lastname: Koshino
authors.forename: R
authors.initials: R
authors.lastname: Okamoto
authors.forename: H E
authors.initials: HE
authors.lastname: Jasin
mesh_terms: D000818:Animals; D002358:Cartilage, Articular / Q000378:metabolism*; D002417:Cattle; D019902:Chondrocytes / Q000378:metabolism*; D005434:Flow Cytometry; D008666:Metalloendopeptidases / Q000378:metabolism*
chemical_list: D008666:Metalloendopeptidases