Single amino acid insertion in loop 4 confers amphotropic murine leukemia virus receptor function upon murine Pit1.
Pit1 is the human receptor for gibbon ape leukemia virus (GALV) and feline leukemia virus subgroup B (FeLV-B), while the related human protein Pit2 is a receptor for amphotropic murine leukemia virus (A-MuLV). The A-MuLV-related isolate 10A1 can utilize both Pit1 and Pit2 as receptors. A stretch of amino acids named region A was identified in Pit1 (residues 550 to 558 in loop 4) as critical for GALV and FeLV-B receptor function. We have here investigated the role of region A in A-MuLV and 10A1 entry. Insertion of a single amino acid in region A of mouse Pit1 resulted in a functional A-MuLV receptor, showing that region A plays a role in A-MuLV infection. Moreover, the downregulation of 10A1 receptor function by changes in region A of human Pit1 indicates that this region is also involved in 10A1 entry. Therefore, region A seems to play a role in infection by all viruses utilizing Pit1 and/or Pit2 as receptors.
Lundorf
M D
MD
Department of Molecular and Structural Biology, University of Aarhus, Aarhus C, Denmark.
Pedersen
F S
FS
O'Hara
B
B
Pedersen
L
L
D016475:3T3 Cells; D000818:Animals; D016466:CHO Cells; D006224:Cricetinae; D006801:Humans; D009052:Leukemia Virus, Murine / Q000502:physiology*; D051379:Mice; D016254:Mutagenesis, Insertional; D011991:Receptors, Virus / Q000235:genetics* / Q000502:physiology*; D013329:Structure-Activity Relationship
D011991:Receptors, Virus; C067981:leukemia virus receptor, gibbon ape