title: Identification of elf1, a beta-spectrin, in early mouse liver development.
abstract: Beta-spectrins play essential roles in cell-cell interactions and in the maintenance of cell polarity. Our aim was to identify beta-spectrin genes important for the establishment of hepatocyte polarity and differentiation. Using subtractive screening of cDNA libraries from early embryonic mouse livers (post-coital days 10, 11, and 12), we have isolated elf1 (embryonic liver fodrin 1), a differentially expressed beta-spectrin or fodrin (betaSpIIsigmaI). Elf1 encodes a 220-amino acid protein with an NH2 terminal actin-binding domain. In situ hybridization studies demonstrate elf1 expression initially in day 10 embryonic heart tissue, then in day 11-11.5 hepatic tissue. These studies suggest that elf1 may play a role in the emergence of hepatocyte polarity during liver development.
authors.affiliation: Department of Medicine, Department of Veterans Affairs Medical Center, Washington, DC 20422, USA. mishra.lopa@washington.va.gov
authors.forename: L
authors.initials: L
authors.lastname: Mishra
authors.forename: T
authors.initials: T
authors.lastname: Cai
authors.forename: A
authors.initials: A
authors.lastname: Levine
authors.forename: D
authors.initials: D
authors.lastname: Weng
authors.forename: E
authors.initials: E
authors.lastname: Mezey
authors.forename: B
authors.initials: B
authors.lastname: Mishra
authors.forename: J
authors.initials: J
authors.lastname: Gearhart
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002454:Cell Differentiation; D016764:Cell Polarity; D002999:Clone Cells; D018076:DNA, Complementary / Q000737:chemistry; D004268:DNA-Binding Proteins / Q000235:genetics / Q000302:isolation & purification*; D018507:Gene Expression Regulation, Developmental; D008099:Liver / Q000166:cytology / Q000196:embryology*; D051379:Mice; D008969:Molecular Sequence Data; D009687:Nuclear Proteins; D014157:Transcription Factors / Q000235:genetics / Q000302:isolation & purification*
chemical_list: D018076:DNA, Complementary; D004268:DNA-Binding Proteins; C512897:ELF1 protein, human; C512896:Elf1 protein, mouse; D009687:Nuclear Proteins; D014157:Transcription Factors