Interleukin-8 participates in angiogenesis in non-small cell, but not small cell carcinoma of the lung.
We examined interleukin-8 (IL-8) production in 17 lung cancer cell lines, IL-8 expression in tumor specimens and IL-8's contribution to tumor-induced angiogenesis in vivo. Eight of 13 non-small cell lung cancer cell lines constitutively produced high levels of IL-8. Four small cell lung cancer cell lines produced little or no IL-8. Immunohistochemical analysis of transbronchial biopsy specimens revealed IL-8 staining within adenocarcinomas (22/32), squamous cell carcinomas (12/21) and large cell carcinomas (2/3), but not within most small cell carcinomas (1/22). Anti-IL-8 antisera blocked tumor angiogenesis by two IL-8 producing cell lines in a mouse model.
Yatsunami
J
J
Research Institute for Disease of the Chest, Faculty of Medicine, Kyushu University, Fukuoka, Japan.
Tsuruta
N
N
Ogata
K
K
Wakamatsu
K
K
Takayama
K
K
Kawasaki
M
M
Nakanishi
Y
Y
Hara
N
N
Hayashi
S
S
D000230:Adenocarcinoma / Q000098:blood supply; D000818:Animals; D002289:Carcinoma, Non-Small-Cell Lung / Q000098:blood supply*; D018288:Carcinoma, Small Cell / Q000098:blood supply*; D002294:Carcinoma, Squamous Cell / Q000098:blood supply; D002455:Cell Division / Q000187:drug effects; D016209:Interleukin-8 / Q000494:pharmacology / Q000502:physiology*; D008175:Lung Neoplasms; D051379:Mice; D009368:Neoplasm Transplantation; D009389:Neovascularization, Pathologic; D014183:Transplantation, Heterologous; D014407:Tumor Cells, Cultured; D001616:beta-Galactosidase / Q000097:blood*
D016209:Interleukin-8; D001616:beta-Galactosidase