title: Re-evaluating the role of His-143 in the mechanism of type I dehydroquinase from Escherichia coli using two-dimensional 1H,13C NMR.
abstract: Type I dehydroquinase from the shikimate pathway of Escherichia coli dehydrates dehydroquinate to dehydroshikimate. pH/log Vmax profiles of the enzyme indicate the presence of a single ionizing group with a pKa of 6.2. Chemical modification experiments with diethyl pyrocarbonate have identified the conserved residue His-143 as essential for catalysis in this enzyme and the pKa for this modification is also 6.2, implying that this is the single ionizing residue in dehydroquinase that may be acting as a general base in the catalytic mechanism. Subsequent mutagenesis of this residue (Leech, A. P., James, R., Coggins, J. R., and Kleanthous, C. (1995) J. Biol. Chem. 270, 25827-25836) further suggested that His-143 may be involved in Schiff base formation/breakdown as well as being the proton abstracting general base. The importance of this residue was confirmed by recent x-ray crystallographic data showing His-143 to be at the center of a hydrogen-bonded triad, flanked by the essential Schiff base forming residue Lys-170 and Glu-86. In the present study, we have used mutagenesis and 1H and 13C NMR to assign the resonance of His-143 and probe its ionization state to define more precisely its role in the mechanism of type I dehydroquinase. Following isotopic enrichment of wild-type and H143A dehydroquinase enzymes with [2-13C]histidine, the resonance for His-143 was assigned by comparing their 1H,13C heteronuclear single quantum correlation NMR spectra. pH titrations revealed that whether in the liganded or unliganded state, His-143 does not ionize over the pH range 6-9.5 and so cannot possess a pKa of 6.2. The NMR data are consistent with this residue remaining unprotonated at pH values optimal for the activity of this enzyme (pH > 7). The role of His-143 is re-evaluated in light of these and the recent structural data, and an alternative candidate for the pKa of 6.2 is discussed.
authors.affiliation: School of Biological Sciences, University of East Anglia, Norwich NR4 7TJ, United Kingdom.
authors.forename: A P
authors.initials: AP
authors.lastname: Leech
authors.forename: R
authors.initials: R
authors.lastname: Boetzel
authors.forename: C
authors.initials: C
authors.lastname: McDonald
authors.forename: A K
authors.initials: AK
authors.lastname: Shrive
authors.forename: G R
authors.initials: GR
authors.lastname: Moore
authors.forename: J R
authors.initials: JR
authors.lastname: Coggins
authors.forename: L
authors.initials: L
authors.lastname: Sawyer
authors.forename: C
authors.initials: C
authors.lastname: Kleanthous
mesh_terms: D002247:Carbon Isotopes; D018360:Crystallography, X-Ray; D004926:Escherichia coli / Q000201:enzymology*; D006639:Histidine; D006836:Hydro-Lyases / Q000737:chemistry* / Q000378:metabolism; D006859:Hydrogen; D006860:Hydrogen Bonding; D007700:Kinetics; D008968:Molecular Conformation; D016297:Mutagenesis, Site-Directed; D019906:Nuclear Magnetic Resonance, Biomolecular / Q000379:methods; D017354:Point Mutation; D017433:Protein Structure, Secondary; D011994:Recombinant Proteins / Q000737:chemistry / Q000378:metabolism; D012545:Schiff Bases; D012765:Shikimic Acid / Q000031:analogs & derivatives / Q000737:chemistry / Q000378:metabolism
chemical_list: D002247:Carbon Isotopes; D011994:Recombinant Proteins; D012545:Schiff Bases; C096387:3-dehydroshikimate; D012765:Shikimic Acid; D006639:Histidine; D006859:Hydrogen; D006836:Hydro-Lyases; C020000:3-dehydroquinate dehydratase