title: Characterization and intracellular localization of the Rok1 protein involved in yeast cell division.
abstract: The ROK1 gene is essential for the cell cycle progression in Saccharomyces cerevisiae. ROK1 has been predicted to encode an ATP-dependent RNA helicase of the DEAD-box family. We have analyzed the ROK1 gene expression both at the protein and RNA levels. Polyclonal antibodies were raised against trpE::rok1 hybrid proteins and were affinity purified by using lacZ::rok1 hybrid proteins. Western blot experiments using anti-Rok1 antibodies revealed a single protein band of 64 kDa which is an expected size from the Rok1 amino acid sequence. Indirect immuno-fluorescence showed that the Rok1 protein is localized predominantly to the cytoplasm of the vegetatively growing cells. We have detected immunocross-reactive homologs of Rok1p in Candida albicans and Drosophila melanogaster.
authors.affiliation: Department of Microbiology, College of Natural Sciences, Chungnam National University, Taejeon, Korea.
authors.forename: J Y
authors.initials: JY
authors.lastname: Rhee
authors.forename: W B
authors.initials: WB
authors.lastname: Lee
authors.forename: J
authors.initials: J
authors.lastname: Kim
mesh_terms: D000818:Animals; D000908:Antibodies, Fungal / Q000096:biosynthesis; D000946:Antigens, Fungal / Q000276:immunology; D002176:Candida albicans / Q000276:immunology; D002455:Cell Division; D003429:Cross Reactions; D003593:Cytoplasm / Q000378:metabolism; D004331:Drosophila melanogaster / Q000276:immunology; D005656:Fungal Proteins / Q000276:immunology; D019476:Insect Proteins / Q000276:immunology; D020365:RNA Helicases; D012316:RNA Nucleotidyltransferases / Q000737:chemistry* / Q000235:genetics / Q000276:immunology / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000166:cytology* / Q000201:enzymology* / Q000235:genetics; D014158:Transcription, Genetic
chemical_list: D000908:Antibodies, Fungal; D000946:Antigens, Fungal; D005656:Fungal Proteins; D019476:Insect Proteins; D012316:RNA Nucleotidyltransferases; D020365:RNA Helicases