title: Antibacterial steroidal alkaloids from Sarcococca saligna.
abstract: Two new pregnane-type steroidal alkaloids, saligcinnamide [(20S,2'E)-20-(N,N-dimethylamino)-3beta-(3'-phenyl-2'-propenyl-N-meth ylamido)pregnane](1) and N(a)-methyl epipachysamine-D [(20S)-20-(N,N-dimethylamino)-3beta-(N-methylbenzamido)pregnane](2 ), along with a known base, epipachysamine D [(20S)-20-(N,N-dimethylamino)-3beta-(benzamido)pregnane] (3), were isolated from the EtOH extracts of the roots and stems of Sarcococca saligna. The new bases exhibited antibacterial activity against several human pathogenic bacteria. Two derivatives of 1, dihydrosaligcinnarnide [(20S)-20-(N,N-dimethylamino)-3beta-(3'-phenylpropionoyl-N-meth ylamido)pregnane](4) and dihydrosaligcinnamine [(20S)-20-(N,N-dimethylamino)-3beta-N-(3'-phenylpropyl-N-methylamino)pre gnane](5), and a derivative of 2, N(a)-methyl epipachysamine [(20S)-20-(N,N-dimethylamino)-3beta-(N-benzyl,N-methylamino)pregnane](6) were prepared and their antibacterial activity determined.
authors.affiliation: International Centre for Chemical Sciences at H. E. J. Research Institute of Chemistry, University of Karachi, Pakistan.
authors.forename: A
authors.initials: A
authors.lastname: Rahman
authors.forename: S
authors.initials: S
authors.lastname: Anjum
authors.forename: A
authors.initials: A
authors.lastname: Farooq
authors.forename: M R
authors.initials: MR
authors.lastname: Khan
authors.forename: Z
authors.initials: Z
authors.lastname: Parveen
authors.forename: M I
authors.initials: MI
authors.lastname: Choudhary
mesh_terms: D000470:Alkaloids / Q000302:isolation & purification* / Q000494:pharmacology; D000900:Anti-Bacterial Agents / Q000302:isolation & purification* / Q000494:pharmacology; D001419:Bacteria / Q000187:drug effects; D009682:Magnetic Resonance Spectroscopy; D013058:Mass Spectrometry; D008826:Microbial Sensitivity Tests; D008968:Molecular Conformation; D010154:Pakistan; D010946:Plants, Medicinal / Q000737:chemistry*; D016339:Spectrometry, Mass, Fast Atom Bombardment; D013056:Spectrophotometry, Ultraviolet
chemical_list: D000470:Alkaloids; D000900:Anti-Bacterial Agents