Inhibition by glass-ionomer cements of protein synthesis by human gingival fibroblasts in continuous culture.
The effects of several glass-ionomer cements (Baseline and Chem Fil of De Trey; Ketac Fil and Ketac Bond of ESPE; Vitrebond and Vitremer of 3 M) on the protein synthesis of cultured gingival fibroblasts were studied. The presence of cements in the culture medium inhibited protein synthesis, although the relative effectiveness varied significantly. The cements tested have been ranked in three groups, group A (Chem Fil and Ketac Fil), group B (Ketac Bond and Baseline) and group C (Vitrebond and Vitremer), showing 50, 75 and 100% reduction in protein synthesis, respectively. Incubation of cells with medium previously conditioned by Baseline and Ketac Fil caused only transient inhibition of protein synthesis followed by almost complete recovery. This recovery was not observed when the medium was conditioned with Vitrebond. A characterization of the factors determining the inhibitory effect of one cement in each group was attempted. The cements, irrespective of the type, produced small but reproducible decreases in the pH of the medium but released fluoride ions to a different extent. Both changes in the pH of the medium and in the fluoride concentration inhibited protein synthesis by cultured gingival fibroblasts. The different action of cements can be explained, at least in part, by a differing release of fluoride ions.
Consiglio
R
R
Istituto di Discipline Odontostomatologiche, Facoltà di Medicina e Chirurgia, Italy.
Rengo
S
S
Liguoro
D
D
Riccitiello
F
F
Formisano
S
S
Palumbo
G
G
Di Jeso
B
B
D000538:Aluminum Silicates / Q000737:chemistry / Q000494:pharmacology; D002478:Cells, Cultured; D003188:Composite Resins / Q000737:chemistry / Q000494:pharmacology; D003470:Culture Media; D017077:Culture Media, Conditioned; D004305:Dose-Response Relationship, Drug; D005347:Fibroblasts / Q000187:drug effects* / Q000378:metabolism; D005459:Fluorides / Q000008:administration & dosage / Q000737:chemistry / Q000494:pharmacology; D005881:Gingiva / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D005899:Glass Ionomer Cements / Q000737:chemistry / Q000494:pharmacology*; D006801:Humans; D006863:Hydrogen-Ion Concentration; D008298:Maleates / Q000737:chemistry / Q000494:pharmacology; D008422:Materials Testing; D014176:Protein Biosynthesis; D011506:Proteins / Q000037:antagonists & inhibitors*; D015203:Reproducibility of Results
D000538:Aluminum Silicates; D003188:Composite Resins; D003470:Culture Media; D017077:Culture Media, Conditioned; D005899:Glass Ionomer Cements; D008298:Maleates; D011506:Proteins; C084550:Vitremer; C061499:Ketac-Bond; C074807:BaseLine dental cement; C067790:Vitrabond; C039733:Chemfil; C040964:Ketac-Fil; D005459:Fluorides