title: Detection of human papillomavirus in verrucous carcinoma from HIV-seropositive patients.
abstract: Anogenital squamous cell carcinoma has been noted with increased frequency in HIV-seropositive patients. Verrucous carcinoma is a variant of squamous cell carcinoma that tends to be locally invasive and non-metastasizing. Although human papillomavirus (HPV) has been strongly implicated in other squamous neoplasms, it has been variably associated with verrucous carcinoma and has not been examined in these lesions in the HIV-positive population. The aim of this study was to examine the association of HPV with anal verrucous carcinoma in patients with the human immunodeficiency virus (HIV). HPV DNA in situ hybridization for HPV Types 6/11, 16/18, and 31/33/35 was performed on formalin-fixed, paraffin-embedded tissue from six cases of verrucous carcinoma and four cases of condyloma acuminatum in perianal specimens from HIV-seropositive patients. HPV DNA sequences were identified in five of six cases of verrucous carcinoma and in all cases of condyloma acuminatum. Of the five verrucous carcinomas that harbored detectable HPV DNA, four contained HPV 6/11 and two contained HPV 16/18. One contained both HPV 6/11 and HPV 16/18. All four cases of condyloma acuminatum were positive for HPV 6/11. One patient included in this series had three chronologically separate verrucous carcinomas. The initial lesion was negative for HPV DNA. Subsequent verrucous carcinomas were positive for HPV type 6/11 and type 16/18, respectively. The data presented support the concept that verrucous carcinoma in the HIV-seropositive population is associated with HPV, which may indeed play an important role in its pathogenesis.
authors.affiliation: Department of Pathology, Anatomy, and Cell Biology, Thomas Jefferson University Hospital, Philadelphia, Pennsylvania 19107, USA.
authors.forename: K H
authors.initials: KH
authors.lastname: Cuesta
authors.forename: J P
authors.initials: JP
authors.lastname: Palazzo
authors.forename: K R
authors.initials: KR
authors.lastname: Mittal
mesh_terms: D000328:Adult; D018289:Carcinoma, Verrucous / Q000150:complications* / Q000473:pathology / Q000821:virology*; D004279:DNA, Viral / Q000032:analysis; D006679:HIV Seropositivity / Q000150:complications*; D006801:Humans; D017403:In Situ Hybridization; D008875:Middle Aged; D027383:Papillomaviridae / Q000235:genetics / Q000302:isolation & purification*; D012878:Skin Neoplasms / Q000150:complications* / Q000473:pathology / Q000821:virology*
chemical_list: D004279:DNA, Viral