title: Major laminin-binding and F-actin-linked glycoproteins of neutrophils.
abstract: Two major EHS-laminin-binding membrane glycoproteins--with apparent molecular masses of 50 kD and 18 kD--were shown by protein blotting in membrane fractions of porcine neutrophils. These galectin-like glycoproteins (binding probably via the N-acetyllactosamine sequences to laminin) could also be detected by labelled F-actin in protein blots. Following 35 min adhesion to the plastic surface, the relative amount of the 18 kD protein increased considerably in the light (plasma membrane) and in the dense (intracellular) membrane fractions of the attached cells; the 50 kD polypeptide (identified as a CD14-like protein) seemed to accumulate characteristically in the dense membrane fraction. These observations imply that direct connections could be formed between membrane glycoproteins and microfilaments during cell-substrate adhesion which may be preceded by enhanced cell surface targeting of certain adhesion receptors.
authors.affiliation: Department of Biochemistry, Albert Szent-Györgyi Medical University, Hungary.
authors.forename: M
authors.initials: M
authors.lastname: Keresztes
authors.forename: Z
authors.initials: Z
authors.lastname: Lajtos
mesh_terms: D000199:Actins / Q000032:analysis; D000818:Animals; D002352:Carrier Proteins / Q000097:blood*; D002448:Cell Adhesion; D002462:Cell Membrane / Q000648:ultrastructure; D007425:Intracellular Membranes / Q000648:ultrastructure; D007797:Laminin / Q000378:metabolism; D008562:Membrane Glycoproteins / Q000097:blood*; D051379:Mice; D008840:Microfilament Proteins / Q000097:blood*; D008970:Molecular Weight; D009504:Neutrophils / Q000737:chemistry* / Q000648:ultrastructure*; D011498:Protein Precursors; D017474:Receptors, Laminin / Q000097:blood*; D013552:Swine; D014407:Tumor Cells, Cultured
chemical_list: D000199:Actins; D002352:Carrier Proteins; C034037:F-actin-binding proteins; D007797:Laminin; D008562:Membrane Glycoproteins; D008840:Microfilament Proteins; D011498:Protein Precursors; D017474:Receptors, Laminin