Antitumor effect of human pancreatic cancer cells transduced with cytokine genes which activate Th1 helper T cells.
We have examined antitumor effect of human pancreatic carcinoma cells (AsPC-1) retrovirally transduced with interleukin-12 (IL-12), IL-15 or IL-18 gene in nude mice. The tumor growth of IL-12-expressing AsPC-1 cells was significantly retarded and that of IL-15-expressing cells was also impeded compared with that of wild-type cells, although their in vitro cell growth remained unchanged. However, the expression of IL-18 in AsPC-1 cells did not generate any antitumor effect since the tumor growth of the transduced cells was the same as that of wild-type cells. Thus, the differential actions of these cytokines on non-T cells can generate a variety of antitumor effect in nude mice, although their actions on T cells lineage favor the stimulation of Th1-type helper T cells.
Yoshida
Y
Y
Division of Pathology, Chiba Cancer Center Research Institute, Japan.
Tasaki
K
K
Kimurai
M
M
Takenaga
K
K
Yamamoto
H
H
Yamaguchi
T
T
Saisho
H
H
Sakiyama
S
S
Tagawa
M
M
D000818:Animals; D002277:Carcinoma / Q000276:immunology* / Q000473:pathology; D016207:Cytokines / Q000502:physiology*; D005260:Female; D006801:Humans; D018664:Interleukin-12 / Q000502:physiology*; D019409:Interleukin-15 / Q000502:physiology*; D020382:Interleukin-18; D008213:Lymphocyte Activation; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D010190:Pancreatic Neoplasms / Q000276:immunology* / Q000473:pathology; D018417:Th1 Cells / Q000276:immunology*; D014161:Transduction, Genetic; D014407:Tumor Cells, Cultured
D016207:Cytokines; D019409:Interleukin-15; D020382:Interleukin-18; D018664:Interleukin-12