Specific cellular and humoral immune response in Balb/c mice immunised with an expression genomic library of Trypanosoma cruzi.
An expression genomic library of Trypanosoma cruzi (T. cruzi) constructed using pcDNA3 plasmid was used for the immunisation (25 micrograms) of Balb/c mice. Expression of T. cruzi antigens in the muscle of inoculated mice was detected by indirect immunofluorescence 7 days after immunisation. Specific IgG antibodies were significatively increased (P < 0.05) in animals that were reimmunized with 50 micrograms of the genomic library. An antigen specific lymphoproliferative response was detected in one animal of the group inoculated with one dose of the library.
Alberti
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E
Department of Protozoology, Tropical Medicine Institute Pedro Kouri, Ciudad Habana, Cuba.
Acosta
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A
Sarmiento
M E
ME
Hidalgo
C
C
Vidal
T
T
Fachado
A
A
Fonte
L
L
Izquierdo
L
L
Infante
J F
JF
Finlay
C M
CM
Sierra
G
G
D000818:Animals; D000917:Antibody Formation / Q000276:immunology; D000918:Antibody Specificity; D016054:DNA, Protozoan / Q000235:genetics / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D018503:Genome, Protozoan; D007111:Immunity, Cellular / Q000276:immunology; D007074:Immunoglobulin G / Q000096:biosynthesis / Q000097:blood / Q000276:immunology; D008213:Lymphocyte Activation / Q000276:immunology; D051379:Mice; D008807:Mice, Inbred BALB C; D016052:Protozoan Vaccines / Q000235:genetics / Q000276:immunology / Q000494:pharmacology*; D014349:Trypanosoma cruzi / Q000235:genetics* / Q000276:immunology*; D019444:Vaccines, DNA / Q000235:genetics / Q000276:immunology / Q000494:pharmacology*
D016054:DNA, Protozoan; D007074:Immunoglobulin G; D016052:Protozoan Vaccines; D019444:Vaccines, DNA