title: Pseudomonas cellulose-binding domains mediate their effects by increasing enzyme substrate proximity.
abstract: To investigate the mode of action of cellulose-binding domains (CBDs), the Type II CBD from Pseudomonas fluorescens subsp. cellulosa xylanase A (XYLACBD) and cellulase E (CELECBD) were expressed as individual entities or fused to the catalytic domain of a Clostridium thermocellum endoglucanase (EGE). The two CBDs exhibited similar Ka values for bacterial microcrystalline cellulose (CELECBD, 1.62x10(6) M-1; XYLACBD, 1.83x10(6) M-1) and acid-swollen cellulose (CELECBD, 1.66x10(6) M-1; XYLACBD, 1.73x10(6) M-1). NMR spectra of XYLACBD titrated with cello-oligosaccharides showed that the environment of three tryptophan residues was affected when the CBD bound cellohexaose, cellopentaose or cellotetraose. The Ka values of the XYLACBD for C6, C5 and C4 cello-oligosaccharides were estimated to be 3.3x10(2), 1.4x10(2) and 4.0x10(1) M-1 respectively, suggesting that the CBD can accommodate at least six glucose molecules and has a much higher affinity for insoluble cellulose than soluble oligosaccharides. Fusion of either the CELECBD or XYLACBD to the catalytic domain of EGE potentiated the activity of the enzyme against insoluble forms of cellulose but not against carboxymethylcellulose. The increase in cellulase activity was not observed when the CBDs were incubated with the catalytic domain of either EGE or XYLA, with insoluble cellulose and a cellulose/hemicellulose complex respectively as the substrates. Pseudomonas CBDs did not induce the extension of isolated plant cell walls nor weaken cellulose paper strips in the same way as a class of plant cell wall proteins called expansins. The XYLACBD and CELECBD did not release small particles from the surface of cotton. The significance of these results in relation to the mode of action of Type II CBDs is discussed.
authors.affiliation: Department of Biological and Nutritional Sciences, University of Newcastle upon Tyne, Newcastle upon Tyne NE1 7RU, UK.
authors.forename: D N
authors.initials: DN
authors.lastname: Bolam
authors.forename: A
authors.initials: A
authors.lastname: Ciruela
authors.forename: S
authors.initials: S
authors.lastname: McQueen-Mason
authors.forename: P
authors.initials: P
authors.lastname: Simpson
authors.forename: M P
authors.initials: MP
authors.lastname: Williamson
authors.forename: J E
authors.initials: JE
authors.lastname: Rixon
authors.forename: A
authors.initials: A
authors.lastname: Boraston
authors.forename: G P
authors.initials: GP
authors.lastname: Hazlewood
authors.forename: H J
authors.initials: HJ
authors.lastname: Gilbert
mesh_terms: D001426:Bacterial Proteins / Q000737:chemistry; D001665:Binding Sites / Q000235:genetics; D002480:Cellulase / Q000737:chemistry* / Q000235:genetics; D002482:Cellulose / Q000378:metabolism*; D003013:Clostridium / Q000201:enzymology; D043364:Endo-1,4-beta Xylanases; D007700:Kinetics; D009682:Magnetic Resonance Spectroscopy; D009844:Oligosaccharides / Q000378:metabolism; D011551:Pseudomonas fluorescens / Q000201:enzymology*; D011993:Recombinant Fusion Proteins / Q000235:genetics; D014995:Xylosidases / Q000737:chemistry*
chemical_list: D001426:Bacterial Proteins; D009844:Oligosaccharides; D011993:Recombinant Fusion Proteins; C099423:cellohexaose; D002482:Cellulose; D014995:Xylosidases; D002480:Cellulase; D043364:Endo-1,4-beta Xylanases