Localization of potential binding sites for the edema disease verotoxin (VT2e) in pigs.
The purpose of this study was to identify organs and cells to which the edema disease verotoxin (VT2e) could bind in pigs. Frozen 4-5 microns thick sections of organs usually affected in edema disease (colon, spinal cord, cerebellum and eyelid) and organs not usually affected (liver, ileum) from two 5- to 6-week-old weaned pigs were permeabilized with acetone, then exposed to VT2e. Unbound VT2e was removed by washing and bound VT2e was detected by immunohistochemistry. In the eyelid, double-label immunofluorescence was used to identify the cells to which VT2e bound. VT2e was shown to bind to all six organs that were examined. The toxin bound to arteries in all organs, to veins in all organs except the liver, and to enterocytes in the ileal crypts. Double labelling of eyelid with monoclonal antibodies specific for von Willebrand factor or alpha-smooth actin and VT2e showed that the toxin bound to endothelial and vascular smooth muscle cells. The binding of VT2e to endothelium is consistent with findings for other verotoxins but binding to vascular smooth muscle has not been reported for other verotoxins. It is concluded that i) factors other than the presence of receptors for VT2e influence the development of lesions in edema disease, and ii) smooth muscle necrosis, which is characteristic of the vascular lesions in edema disease, may be due to a direct action of toxin on smooth muscle cells.
Waddell
T E
TE
Department of Pathobiology, Ontario Veterinary College, University of Guelph.
Coomber
B L
BL
Gyles
C L
CL
D000818:Animals; D001427:Bacterial Toxins / Q000493:pharmacokinetics*; D001665:Binding Sites; D004488:Edema Disease of Swine / Q000378:metabolism* / Q000473:pathology; D004730:Endothelium, Vascular / Q000378:metabolism* / Q000473:pathology; D004768:Enterotoxins / Q000493:pharmacokinetics; D004926:Escherichia coli; D007150:Immunohistochemistry; D009131:Muscle, Smooth, Vascular / Q000378:metabolism* / Q000473:pathology; D022641:Shiga Toxin 2; D013552:Swine; D014018:Tissue Distribution
D001427:Bacterial Toxins; D004768:Enterotoxins; D022641:Shiga Toxin 2