Release of the soluble interleukin-6 receptor from human T-cells.
The soluble Interleukin-6 receptor (sIL-6R) is capable of conferring the Interleukin-6 (IL-6) signal onto cells lacking the gp80 ligand binding protein. Here we investigate the release of sIL-6R from T-cells. After 2 h stimulation with PMA, a release of sIL-6R from peripheral human T-cells was observed which was insensitive to the protein synthesis inhibitor cycloheximide. This release was accompanied by a decrease of membrane-bound (mb) IL-6R. After 24 h, however, the observed sIL6-R release did prove to be sensitive to cycloheximide. These results suggest that both shedding and denovo-synthesis may be responsible for the PMA-induced sIL-6R release. In contrast to PMA, neither anti-CD3, a positive, nor IL-10, a negative regulator of IL-6 release from T-cells affected the production of the sIL-6R. The differential regulation of sIL-6R and IL-6 production by T-cells might be relevant for the immunomodulatory potential of the sIL-6R with respect to the interaction of T- and non-T-cells.
Banning
U
U
Department of Pediatric Hematology and Oncology, Heinrich-Heine University, Düsseldorf, Germany.
Bönig
H
H
Pafferath
B
B
Klein-Vehne
A
A
Burdach
S
S
Körholz
D
D
D017252:CD3 Complex / Q000378:metabolism; D003513:Cycloheximide / Q000494:pharmacology; D006801:Humans; D016753:Interleukin-10 / Q000494:pharmacology; D008934:Mitogens / Q000494:pharmacology; D011500:Protein Synthesis Inhibitors / Q000494:pharmacology; D019947:Receptors, Interleukin-6 / Q000378:metabolism*; D012995:Solubility; D013601:T-Lymphocytes / Q000187:drug effects / Q000378:metabolism*; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology
D017252:CD3 Complex; D008934:Mitogens; D011500:Protein Synthesis Inhibitors; D019947:Receptors, Interleukin-6; D016753:Interleukin-10; D003513:Cycloheximide; D013755:Tetradecanoylphorbol Acetate