Protein engineering aspartic proteinases. Site-directed mutagenesis, biochemical characterisation, and X-ray analysis of chymosins with substituted single amino acid substitutions and loop replacements.
Albert
A
A
Department of Crystallography, Birkbeck College, University of London, United Kingdom.
Blundell
T L
TL
Dhanaraj
V
V
Donate
L E
LE
Groves
M
M
Guruprasad
K
K
Nugent
P G
PG
Orprayoon
P
P
Pitts
J E
JE
Rufino
S
S
Srinivasan
N
N
Williams
M
M
Wilsher
J
J
D000596:Amino Acids; D000818:Animals; D012085:Chymosin / Q000737:chemistry* / Q000235:genetics*; D018360:Crystallography, X-Ray; D015870:Gene Expression; D016297:Mutagenesis, Site-Directed; D015202:Protein Engineering / Q000379:methods*; D017433:Protein Structure, Secondary; D011993:Recombinant Fusion Proteins / Q000737:chemistry / Q000235:genetics
D000596:Amino Acids; D011993:Recombinant Fusion Proteins; D012085:Chymosin