Cloning and sequence analysis of the cDNA encoding the elongation factor-1 beta of Dictyostelium discoideum.
A cDNA encoding elongation factor-1 beta (DdEF-1 beta) for protein synthesis was isolated from Dictyostlium discoideum cells by the differential display (DD) method. The DdEF-1 beta cDNA sequence with a length of 733 bp encodes a protein (M(r) 24.1 kDa) consisting of 214 amino acids. Comparison of the predicted amino acid sequence revealed relatively high homology to that of DdEF-1 beta from silkworm (59%), human (57%) and Xenopus (55%). Northern analysis showed that the DdEF-1 beta mRNA is maximally expressed during the vegetative growth phase, followed by marked decrease in response to cell differentiation induced by starvation.
Chae
S
S
Biological Institute, Graduate School of Science, Tohoku University, Sendai, Japan.
Maeda
Y
Y
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D012831:Bombyx; D003001:Cloning, Molecular / Q000379:methods*; D018076:DNA, Complementary / Q000235:genetics*; D004271:DNA, Fungal / Q000235:genetics*; D004023:Dictyostelium / Q000235:genetics*; D005656:Fungal Proteins / Q000235:genetics*; D005815:Genetic Code; D006801:Humans; D008969:Molecular Sequence Data; D020648:Peptide Elongation Factor 1; D010445:Peptide Elongation Factors / Q000235:genetics*; D017386:Sequence Homology, Amino Acid; D014981:Xenopus
D018076:DNA, Complementary; D004271:DNA, Fungal; D005656:Fungal Proteins; D020648:Peptide Elongation Factor 1; D010445:Peptide Elongation Factors