Preparation of immunoliposomes directed against CD34 antigen as target.
The My-10 monoclonal antibody has facilitated the search of haematopoietic stem cells by recognizing selectively the human CD34 antigen. In the present work, My-10 immunoliposomes directed specifically against CD34+ cells were prepared, characterized and tested in vitro. Binding to target cells at 4 degreesC of immunoliposomes containing carboxyfluorescein as aqueous marker was evaluated by flow cytometry and fluorescence microscopy. These immunoliposomes demonstrated their capacity to bind specifically to CD34+ cells. Studies have shown that 9 antibodies/vesicle were sufficient to obtain a good binding efficiency. The product was stable over one month at 4 degreesC in terms of leakage of encapsulated carboxyfluorescein, particle size and antigen binding capacity.
Mercadal
M
M
Department of Biochemistry and Molecular Biology, Faculty of Chemistry, University of Barcelona, Martí i Franqués, 1, 08028 Barcelona, Spain.
Carrion
C
C
Domingo
J C
JC
Petriz
J
J
Garcia
J
J
de Madariaga
M A
MA
D000911:Antibodies, Monoclonal; D018952:Antigens, CD34 / Q000032:analysis*; D002460:Cell Line; D005434:Flow Cytometry; D005452:Fluoresceins; D005456:Fluorescent Dyes; D006412:Hematopoietic Stem Cells / Q000737:chemistry*; D006801:Humans; D008081:Liposomes; D008856:Microscopy, Fluorescence
D000911:Antibodies, Monoclonal; D018952:Antigens, CD34; D005452:Fluoresceins; D005456:Fluorescent Dyes; D008081:Liposomes; C024098:6-carboxyfluorescein