Oligonucleotide DNA fingerprinting optimized to determine parentage in three beetle species.
Oligonucleotide fingerprinting was performed on three beetle species from different families (Coleoptera: Staphylinidae: Aleochara curtula; Silphidae: Nicrophorus vespilloides; Tenebrionidae: Blaps lethifera) to obtain detailed information on parentage in mating systems. We report variations in the number of hybridizing fragments for the different species depending on the combinations of probes and restriction enzymes used. In addition to conventional multilocus fingerprint patterns, we established a single locus system for A. curtula (GTG5; HinfI) and described an oligolocus system in N. vespilloides (GATA4; HaeIII).
Benken
T
T
Institut für Biologie I (Zoologie), Albert-Ludwigs-Universität, Freiburg, Germany. benken@sun2.ruf.uni-freiburg.de
Müller
J K
JK
Peschke
K
K
D000818:Animals; D002874:Chromosome Mapping; D001517:Coleoptera / Q000235:genetics*; D016172:DNA Fingerprinting / Q000379:methods*; D004587:Electrophoresis, Agar Gel; D005260:Female; D014644:Genetic Variation; D008297:Male; D009841:Oligonucleotides; D013045:Species Specificity; D013712:Tenebrio / Q000235:genetics*
D009841:Oligonucleotides