Investigating the relaxation, following diazo-2 laser flash photolysis, of a skinned trabecular preparation from SHR hypertrophied left ventricle.
Rat models of cardiac hypertrophy are characterised by a shift in left ventricular myosin isoform from V1 (adult) to V3 (foetal), the latter being associated with a slowing of the acto-myosin ATPase rate. The aim of this study was to examine hypertrophy effects on relaxation by investigating a chemically skinned cardiac preparation from the SHR, where all the cellular membranes are rendered non-functional allowing the myofibrils to be studied in isolation. On comparison, following photolysis of the photolabile caged Ca2+ chelator diazo-2, it can be seen that the SHR fibres relax at a slower rate than their age-matched WKY counterparts. We suggest that, since the thin filament regulatory proteins seem not to be affected by cardiac hypertrophy in the rat, this result can be attributed to the shift in left ventricular myosin isoforms. The reduced relaxation rate in the SHR could be the result of a slowing of the dissociation of actin and myosin during the cross-bridge cycle. These results have previously been published in abstract form [1].
Johns
E C
EC
University Laboratory of Physiology, Parks Road, Oxford, OX1 3PT, UK.
Hodson
E A
EA
Fish
H
H
Lymn
J S
JS
Thurston
H
H
Mulligan
I P
IP
Ashley
C C
CC
D000199:Actins / Q000378:metabolism; D000818:Animals; D002118:Calcium / Q000494:pharmacology; D002614:Chelating Agents; D003979:Diazonium Compounds; D006973:Hypertension / Q000503:physiopathology*; D017379:Hypertrophy, Left Ventricular / Q000503:physiopathology*; D007834:Lasers; D009200:Myocardial Contraction; D009218:Myosins / Q000378:metabolism; D010642:Phenoxyacetates; D010782:Photolysis; D051381:Rats; D011918:Rats, Inbred SHR; D011921:Rats, Inbred WKY
D000199:Actins; D002614:Chelating Agents; D003979:Diazonium Compounds; D010642:Phenoxyacetates; C063685:diazo-2; D009218:Myosins; D002118:Calcium