E31-K352, the minimal cation binding moiety of Na+,K(+)-ATPase.
Upon limited tryptic fragmentation of Na+,K(+)-ATPase a 35 kDa fragment (E31-K352) was formed that bound 204Tl+ on blot. Further fragmentation led to loss of binding, pointing to the conclusion that E31-K352 is the minimal cation binding unit in Na+,K(+)-ATPase.
Schuurmans Stekhoven
F M
FM
Department of Biochemistry, University of Nijmegen, The Netherlands.
D000818:Animals; D002412:Cations / Q000378:metabolism*; D004591:Electrophoresis, Polyacrylamide Gel; D007668:Kidney / Q000201:enzymology; D008565:Membrane Proteins / Q000737:chemistry; D008970:Molecular Weight; D010446:Peptide Fragments / Q000737:chemistry*; D011485:Protein Binding; D011817:Rabbits; D000254:Sodium-Potassium-Exchanging ATPase / Q000737:chemistry*; D013691:Tellurium / Q000378:metabolism; D014357:Trypsin / Q000378:metabolism
D002412:Cations; D008565:Membrane Proteins; D010446:Peptide Fragments; D014357:Trypsin; D000254:Sodium-Potassium-Exchanging ATPase; D013691:Tellurium