[Use of western blot methods for serodiagnosis of hydatidosis in Poland].
46 sera and 1 cerebrospinal fluid samples from 47 patients suspected of hydatidosis were examined by Western blot method, and the results were compared with those of the indirect haemagglutination, immunoelectrophoresis and an enzyme-linked immunosorbent assay (ELISA). The examinations confirmed high sensitivity and specificity of the reactions with 8 kDa antigenic fraction in the Western blot. The above data supported the concept, that serological diagnosis of hydatidosis should be carried out using a high sensitivity screening test, such as ELISA, then the Western blot method to verify positive results.
Bitkowska
E
E
Zakład Parazytologii Lekarskiej, PZH, Warszawa.
Gołab
E
E
Płonka
W
W
Dzbeński
T H
TH
D015153:Blotting, Western / Q000379:methods*; D002555:Cerebrospinal Fluid / Q000382:microbiology; D004443:Echinococcosis / Q000175:diagnosis*; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D008403:Mass Screening / Q000379:methods*; D011044:Poland; D012680:Sensitivity and Specificity; D012698:Serologic Tests / Q000379:methods*