Discrimination among the clinical isolates of Candida albicans by amplification of the repetitive sequences, alts.
A primer pair, PB and BSH, which amplified alts, a portion of Candida albicans-specific repetitive sequence, RPS, gave stable and reproducible fingerprint patterns of the strains by polymerase chain reaction (PCR). We applied this method to clinical isolates of C. albicans for strain discrimination. Using PCR fingerprint patterns, we could analyze the relatedness of C. albicans strains including those isolated from children with leukemia and their bedside parents. The results indicated that PCR analysis targeting an alt region gives rise to the same conclusion as the previous study obtained by SmaI RFLP analysis.
Doi
M
M
Laboratory of Medical Mycology, Research Institute for Disease Mechanism and Control, Nagoya University School of Medicine, Aichi, Japan. doim@u-shizuoka-ken.ac.jp
Chibana
H
H
Nakagawa
Y
Y
Tanaka
K
K
D002176:Candida albicans / Q000145:classification* / Q000235:genetics; D002177:Candidiasis / Q000382:microbiology*; D016172:DNA Fingerprinting; D017931:DNA Primers; D006801:Humans; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length; D012091:Repetitive Sequences, Nucleic Acid
D017931:DNA Primers