Identification of target cells by immunohistochemical detection of covalently rearranged estradiol in rehydrated paraffin sections.
Estradiol is released from the binding niche of the receptor and covalently arrested in the molecular vicinity by the Mannich reaction during target fixation in acetic acid/formaldehyde. The exposed steroid is freely accessible for appropriate antibodies. It can be visualized in sections by the second antibody/enzyme technique in high resolution and without enhancements.
Jungblut
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PW
Max-Planck-Institut für Experimentelle Endokrinologie, Hannover, Germany. 106001,2503@compuserve.com
Sierralta
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WD
D000818:Animals; D001940:Breast / Q000378:metabolism; D004717:Endometrium / Q000378:metabolism; D004958:Estradiol / Q000008:administration & dosage / Q000378:metabolism*; D005187:Fallopian Tubes / Q000378:metabolism; D005260:Female; D006041:Goats; D006801:Humans; D007124:Immunoenzyme Techniques; D008867:Microtomy; D016612:Paraffin Embedding; D017698:Postmenopause; D051381:Rats; D017207:Rats, Sprague-Dawley; D013552:Swine; D013977:Tibia / Q000378:metabolism
D004958:Estradiol