Substitution of Asn for Ser543 in the large fragment of Taq DNA polymerase increases the efficiency of synthesis of long DNA molecules.
Substitution of Asn for Ser543 in the large fragment of Taq DNA polymerase (Klentaq) increases several times the efficiency of synthesis of long (over 2 kbp) DNA molecules. The difference in the DNA synthesis efficiencies by the mutant and native enzymes increased with the increase in the DNA fragment length.
Ignatov
K B
KB
Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Moscow.
Miroshnikov
A I
AI
Kramarov
V M
VM
D001216:Asparagine / Q000235:genetics / Q000378:metabolism*; D010582:Bacteriophage lambda / Q000235:genetics; D004279:DNA, Viral / Q000096:biosynthesis*; D016297:Mutagenesis, Site-Directed; D012694:Serine / Q000235:genetics / Q000378:metabolism*; D019914:Taq Polymerase / Q000235:genetics / Q000378:metabolism*
D004279:DNA, Viral; D012694:Serine; D001216:Asparagine; D019914:Taq Polymerase