Increased plasma, biliary, and hepatic cholesterol precursors in pigs with ileal autotransplantation-induced malabsorption of cholesterol and bile acids.
BACKGROUND
Small-bowel transplantation impairs intestinal absorptive function for unknown reasons.


METHODS
The proportions of plasma, biliary, and hepatic cholesterol precursors to cholesterol were determined by gas-liquid chromatography after resection of the proximal 75% of the porcine jejunoileum (n = 15) and autotransplantation of the remaining ileum (n = 15) and were related to in vivo absorption and fecal excretion of cholesterol.


RESULTS
Ileal autotransplantation significantly decreased serum (18%; P < 0.05) and liver (7.6%; P < 0.05) cholesterol content, the esterification percentage of serum cholesterol (5.1%; P < 0.0001), and the total amount of cholesterol absorbed (48%; P < 0.05) and increased fecal excretion of bile acids (108%; P < 0.0001), net cholesterol elimination (53%; P < 0.001), and the proportions of plasma (207%; P < 0.0001), biliary (183%; P < 0.0001), and hepatic (114%; P < 0.0001) cholesterol precursors. The increases were most striking for the side-chain-saturated demethylated sterols, cholesterol and lathosterol, and monomethyl sterols, whose bile/liver and plasma/liver ratios were increased in the autotransplantation group. Plasma, biliary, and hepatic precursor proportions were positively related to each other and similarly correlated with fecal bile acids and the net elimination of cholesterol in feces.


CONCLUSIONS
These findings suggest that ileal autotransplantation in pigs with proximal gut resection increased the levels of cholesterol precursor sterols in plasma, bile, and liver mainly due to a bile-acid-malabsorption-induced increase in hepatic synthesis of cholesterol. Enhanced secretion of cholesterol precursors from the liver into the plasma and bile may have contributed to their increased values during the increased rate of cholesterogenesis.
Pakarinen
M P
MP
Second Dept. of Surgery, Helsinki University Central Hospital, University of Helsinki, Finland.
Halttunen
J
J
Kuusanmäki
P
P
Miettinen
T A
TA
D000818:Animals; D001647:Bile Acids and Salts / Q000378:metabolism*; D001659:Biliary Tract / Q000378:metabolism; D002784:Cholesterol / Q000097:blood / Q000378:metabolism*; D002849:Chromatography, Gas; D005243:Feces / Q000737:chemistry; D005260:Female; D007082:Ileum / Q000637:transplantation; D007408:Intestinal Absorption; D008099:Liver / Q000378:metabolism; D008286:Malabsorption Syndromes / Q000378:metabolism*; D013552:Swine
D001647:Bile Acids and Salts; D002784:Cholesterol