A high-resolution genetic map of the nervous locus on mouse chromosome 8.
The nervous (nr) mutant mouse displays two gross recessive traits: both an exaggeration of juvenile hyperactivity and a pronounced ataxia become apparent during the third and fourth postnatal weeks. Using an intersubspecific intercross, we have established a high-resolution map of a segment of mouse chromosome 8 that places the nr locus in a genomic segment defined by D8Rck1 on the centromeric end and D8Mit3 on the telomeric end. This map position places the nr locus within the BALB/cGr congenic region of the C3HeB/ FeJ-nr strain, confirming the accuracy of our study. We used this map position to identify and evaluate three genes-ankyrin 1, cortexin, and farnesyltransferase-as candidates for the nr gene. These three genes were eliminated from consideration but allowed us to establish the conservation of synteny between the region containing the nr locus and a segment of the short arm of human chromosome 8 (8p21-p11.2). Finally, the incomplete penetrance of the nr phenotype led us to perform a screen for modifier loci, and we present evidence that such a nervous modifier locus may exist on mouse chromosome 5.
De Jager
P L
PL
Howard Hughes Medical Institute, Laboratory of Molecular Biology, Rockefeller University, New York, New York 10021, USA.
Harvey
D
D
Polydorides
A D
AD
Zuo
J
J
Heintz
N
N
D000276:Adjuvants, Immunologic / Q000235:genetics; D019883:Alkyl and Aryl Transferases / Q000235:genetics; D000818:Animals; D017487:Ankyrins / Q000235:genetics; D001259:Ataxia / Q000235:genetics; D002874:Chromosome Mapping; D002898:Chromosomes, Human, Pair 8; D051231:Farnesyltranstransferase; D005808:Genes, Recessive; D008040:Genetic Linkage; D005819:Genetic Markers; D006239:Haplotypes; D006801:Humans; D006948:Hyperkinesis / Q000235:genetics; D036341:Intercellular Signaling Peptides and Proteins; D051379:Mice; D008807:Mice, Inbred BALB C; D008809:Mice, Inbred C3H; D008818:Mice, Neurologic Mutants / Q000235:genetics*; D018895:Microsatellite Repeats / Q000235:genetics; D010455:Peptides / Q000235:genetics
D000276:Adjuvants, Immunologic; D017487:Ankyrins; D005819:Genetic Markers; D036341:Intercellular Signaling Peptides and Proteins; D010455:Peptides; C056590:cortexin; D019883:Alkyl and Aryl Transferases; D051231:Farnesyltranstransferase