Expression of adhesion molecules in chronic B-cell lymphoproliferative disorders.
OBJECTIVE
Abnormalities in the expression of cell adhesion molecules (CAM) are thought to influence the patterns of intranodal growth and hematogeneous spread of malignant cells in chronic lymphoproliferative disorders (LPD). Therefore, the characterization of CAM phenotypic profiles of the neoplastic clones in LPD may help to identify distinct subtypes with prognostic implications. In this work we sought to investigate whether the expression of CAM by circulating malignant cells in patients with B-cell LPD differed from that of normal peripheral blood B-lymphocytes (PBL) and whether the observed phenotypic patterns could be correlated to other biological and clinical parameters of known clinical relevance.


METHODS
Peripheral blood mononuclear cells were obtained from 148 patients with B-cell chronic lymphocytic leukemia (B-CLL), 52 with B-cell non-Hodgkin lymphomas (NHL) and 10 with hairy cell leukemia (HCL). The expression of CAM was analyzed by flow cytometry using monoclonal antibodies against CD49d, CD29, CD11a, CD11b, CD11c, CD18, CD62L, CD54 and CD44.


RESULTS
All CAM were detected in normal peripheral blood B-lymphocytes, except CD11c and CD54, which were present in only a minority of cells. Fluorescence mean channel values (FMC) showed that all molecules, with the exception of CD44, were expressed with dim intensity. Emerging patterns of CAM expression, as assessed by FMC values, were observed in different LPD: thus, B-CLL is characterized by a very low expression of CD49d/CD29 and beta 2 integrins. In this disorder, CD49d/CD29, CD11a, and CD54 increase with tumor burden; NHL show high expression of CD29 and CD54; strong expression of all molecules (except CD11a) was found in HCL, particularly CD11c (FMC values 60 times higher than normal). CD62L was faintly expressed in all diagnostic groups, whereas CD11c showed consistently high FMC values.


CONCLUSIONS
The data shows that the phenotypic characterization of LPD can be further refined by the analysis of their patterns of CAM expression which may help to identify distinct subsets within each nosological group.
Lúcio
P J
PJ
Instituto Português de Oncologia de Francisco Gentil, Lisbon, Portugal.
Faria
M T
MT
Pinto
A M
AM
da Silva
M R
MR
Correia Júnior
M E
ME
da Costa
R J
RJ
Parreira
A B
AB
D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D001402:B-Lymphocytes / Q000378:metabolism*; D001853:Bone Marrow / Q000378:metabolism; D018821:CD18 Antigens / Q000096:biosynthesis / Q000097:blood; D015815:Cell Adhesion Molecules / Q000096:biosynthesis* / Q000097:blood; D002908:Chronic Disease; D005260:Female; D005434:Flow Cytometry; D006801:Humans; D018960:Hyaluronan Receptors / Q000096:biosynthesis / Q000097:blood; D039041:Integrin alpha4beta1; D016023:Integrins / Q000096:biosynthesis / Q000097:blood; D018799:Intercellular Adhesion Molecule-1 / Q000096:biosynthesis / Q000097:blood; D019041:L-Selectin / Q000096:biosynthesis / Q000097:blood; D007943:Leukemia, Hairy Cell / Q000097:blood / Q000378:metabolism; D015451:Leukemia, Lymphocytic, Chronic, B-Cell / Q000097:blood / Q000378:metabolism; D008228:Lymphoma, Non-Hodgkin / Q000097:blood / Q000378:metabolism; D008232:Lymphoproliferative Disorders / Q000378:metabolism*; D008297:Male; D008875:Middle Aged; D009369:Neoplasms / Q000097:blood / Q000378:metabolism; D016201:Receptors, Lymphocyte Homing / Q000096:biosynthesis / Q000097:blood
D018821:CD18 Antigens; D015815:Cell Adhesion Molecules; D018960:Hyaluronan Receptors; D039041:Integrin alpha4beta1; D016023:Integrins; D016201:Receptors, Lymphocyte Homing; D018799:Intercellular Adhesion Molecule-1; D019041:L-Selectin