title: Determination of free fatty acids in human bile by high-performance liquid chromatography.
abstract: We developed a high-performance liquid chromatography (HPLC) method for free fatty acids (FFAs) analysis in bile. In this method, FFAs were extracted from bile in a single step using an Isolute ODS cartridge, derivatized with 9-anthryldiazomethane (ADAM). ADAM was chosen because of its high reactivity with carboxylic acid at room temperature. Then, HPLC was used for separating and quantifying FFAs. This method proved to be simple and time-saving. The mean recovery of FFA added to human gallbladder bile was 97.6%, and the detection limit was 100-250 pg. Using this method, we determined FFA concentrations in the gallbladder bile of 11 gallstone patients. The mean concentration of total FFA was 0.61 (SD = 0.41) mmol/L, and there was wide variation in the individual FFAs.
authors.affiliation: Department of Hygiene and Preventive Medicine, Niigata University School of Medicine, Japan.
authors.forename: Y
authors.initials: Y
authors.lastname: Hori
authors.forename: K
authors.initials: K
authors.lastname: Nakamura
authors.forename: M
authors.initials: M
authors.lastname: Yamamoto
authors.forename: K
authors.initials: K
authors.lastname: Shimada
authors.forename: H
authors.initials: H
authors.lastname: Nakadaira
authors.forename: N
authors.initials: N
authors.lastname: Shibuya
authors.forename: K
authors.initials: K
authors.lastname: Endoh
authors.forename: K
authors.initials: K
authors.lastname: Ogoshi
mesh_terms: D000328:Adult; D001646:Bile / Q000737:chemistry*; D001649:Bile Duct Diseases; D002769:Cholelithiasis / Q000737:chemistry; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D005227:Fatty Acids / Q000032:analysis*; D005260:Female; D005704:Gallbladder / Q000737:chemistry; D006801:Humans; D008297:Male; D008875:Middle Aged
chemical_list: D005227:Fatty Acids