title: Cryopreservation and orthotopic transplantation of mouse ovaries: new approach in gamete banking.
abstract: Mouse half ovaries were cryopreserved and orthotopically transplanted into ovariectomized recipients genetically identical to ovary donors except for the coat color gene. Fertility was reestablished in 57% of the female recipients, which became pregnant in an average of 40 days after transplantation of frozen-thawed half ovaries. These experiments demonstrate that ovary cryopreservation can be a very useful option for banking mouse germplasm, or managing subfertile animal colonies, when embryo or sperm freezing cannot be used or is not cost effective.
authors.affiliation: The Jackson Laboratory, Bar Harbor, Maine 04609, USA. jms@jax.org
authors.forename: J
authors.initials: J
authors.lastname: Sztein
authors.forename: H
authors.initials: H
authors.lastname: Sweet
authors.forename: J
authors.initials: J
authors.lastname: Farley
authors.forename: L
authors.initials: L
authors.lastname: Mobraaten
mesh_terms: D000818:Animals; D015925:Cryopreservation; D005260:Female; D051379:Mice; D008810:Mice, Inbred C57BL; D006080:Ovarian Follicle / Q000502:physiology; D010052:Ovariectomy; D010053:Ovary / Q000502:physiology / Q000637:transplantation*; D011247:Pregnancy