title: Chiral and nonchiral determination of ketoprofen in pharmaceuticals by capillary zone electrophoresis.
abstract: The new method for the enantiomeric resolution of various 2-arylpropionic acids by capillary zone electrophoresis (CZE) using heptakis-tri-O-methyl-beta-cyclodextrin as chiral selector was applied to the determination of ketoprofen in different commercially-available pharmaceutical preparations. The analyte was determined under chiral and nonchiral conditions (viz. in the presence and absence of 50 mM heptakis-tri-O-methyl-beta-cyclodextrin in the background electrolyte), with significantly similar results and relative standard deviations from 1.2 to 6.5% in both cases. The limits of detection and determination for the inactive enantiomer, R-(-)-ketoprofen, were calculated to be 7.0 x 10(-7) and 1.6 x 10(-6) M, respectively. The proposed method was successfully used to determine enantiomeric purity in the drugs studied, with results comparable to those provided by the chiral HPLC method.
authors.affiliation: Departament de Química, Facultat de Ciències, Universitat Autònoma de Barcelona, Bellaterra, Spain.
authors.forename: M
authors.initials: M
authors.lastname: Blanco
authors.forename: J
authors.initials: J
authors.lastname: Coello
authors.forename: H
authors.initials: H
authors.lastname: Iturriaga
authors.forename: S
authors.initials: S
authors.lastname: Maspoch
authors.forename: C
authors.initials: C
authors.lastname: Pérez-Maseda
mesh_terms: D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000032:analysis*; D019075:Electrophoresis, Capillary / Q000379:methods*; D007660:Ketoprofen / Q000032:analysis*; D004364:Pharmaceutical Preparations / Q000737:chemistry*; D013237:Stereoisomerism
chemical_list: D000894:Anti-Inflammatory Agents, Non-Steroidal; D004364:Pharmaceutical Preparations; D007660:Ketoprofen