[Phosphorylation of 5'-O-phosphonylmethylthymidine and its incorporation into HeLa cells DNA].
[3H]5'-O-Phosphonylmethylthymidine with a specific activity of 71 Ci/mmol was obtained by isotope exchange. Its incubation with a HeLa cell culture resulted in the formation of [3H]-labeled 5'-O-(beta-phosphoryl-alpha-phosphonylmethyl)thymidine, 5'-O-(beta,gamma-diphosphoryl-alpha-phosphonylmethyl)thymidine, and [3H]DNA. This proved the ability of 5'-O-phosphonylmethylthymidine to undergo phosphorylation and incorporation into the DNA of human cells.
Ias'ko
M V
MV
Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, Moscow, Russia.
Sidorov
G V
GV
Kondratov
R V
RV
Prasolov
V S
VS
Miasoedov
N F
NF
Kraevskiĭ
A A
AA
D004273:DNA, Neoplasm / Q000378:metabolism*; D006367:HeLa Cells; D006801:Humans; D009943:Organophosphorus Compounds / Q000378:metabolism*; D010766:Phosphorylation; D013936:Thymidine / Q000031:analogs & derivatives* / Q000378:metabolism; D014316:Tritium
C112087:5'-O-(beta,gamma-diphosphoryl-alpha-phosphonomethyl)thymidine; C112086:5'-O-(beta-phosphoryl-alpha-phosphonylmethyl)thymidine; C112085:5'-O-phosphonylmethylthymidine; D004273:DNA, Neoplasm; D009943:Organophosphorus Compounds; D014316:Tritium; D013936:Thymidine