Gel electrophoretic detection of 7,8-dihydro-8-oxoguanine and 7, 8-dihydro-8-oxoadenine via oxidation by Ir (IV).
Two gel electrophoretic methods are described for detection of 7, 8-dihydro-8-oxoguanine and 7,8-dihydro-8-oxoadenine based on their further oxidation with one-electron oxidants including IrCl62-and IrBr62-. The products of nucleobase oxidation lead to enhanced piperidine-sensitive cleavage and to highly visible stop points in a primer extension assay. 8-oxoG and 8-oxoA lesions may be distinguished by the latter's inability to be oxidized by IrBr62-compared to IrCl62-Comparison is also made to oxidation by MnO4-.
Muller
J G
JG
Department of Chemistry, University of Utah, 315 South 1400 East, RM Dock, Salt Lake City, UT 84112-0850, USA.
Duarte
V
V
Hickerson
R P
RP
Burrows
C J
CJ
D000225:Adenine / Q000031:analogs & derivatives* / Q000302:isolation & purification; D004249:DNA Damage; D017931:DNA Primers; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods*; D006147:Guanine / Q000031:analogs & derivatives* / Q000302:isolation & purification; D007495:Iridium; D010084:Oxidation-Reduction
D017931:DNA Primers; C010978:hexachloroiridic acid; C002992:8-hydroxyadenine; D007495:Iridium; C024829:8-hydroxyguanine; D006147:Guanine; D000225:Adenine