Inhibition effect of enterocin CCM 4231 in the rumen fluid environment.
Enterocin CCM 4231 is a bacteriocin with a broad antimicrobial spectrum produced by the ruminal strain Enterococcus faecium CCM 4231. Its inhibitory effect towards enterococci, Ent. faecium EF 26/42, staphylococci, Streptococcus bovis AO 24/85 and Escherichia coli, as well as towards Listeria monocytogenes OHIO strain, in the rumen fluid environment was studied during culture at 37 and 30 degrees C for 24 h and 20 days. Enterocin CCM 4231 was added to the samples at a concentration of 3200 AU ml-1. The best inhibitory effect was noted against enterococci at both cultivation temperatures. A decrease in total cell count from 10(8) cfu ml-1/or 10(4) cfu ml-1 and from 10(5) cfu ml-1 to 10(1) cfu ml-1 was detected. Addition of enterocin to the rumen fluid also inhibited staphylococci (from 10(5) cfu ml-1 to 10(4) cfu ml-1 and/or 10(3) cfu ml-1). Gram-negative E. coli cells were inhibited at both cultivation temperatures (decrease from 10(6) cfu ml-1 to 10(1) cfu ml-1 at 37 degrees C, and from 10(7) cfu ml-1 to 10(5) cfu ml-1 at 30 degrees C). Enterococcus faecium EF 26/42 and Streptococcus bovis AO 24/85, the strains growing in the rumen fluid, were the most sensitive to the addition of enterocin during the first 24 h of fermentation (decrease from 10(10) cfu ml-1 and 10(8) cfu ml-1 to 10(6) cfu ml-1 and 10(4) cfu ml-1). An antilisterial effect of the bacteriocin was also confirmed. Further application of bacteriocin in ruminal ecology was indicated.
Lauková
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Institute of Animal Physiology, Slovak Academy of Sciences, Soltésovej, Kosice, Slovakia. laukova@saske.sk
Czikková
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D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D001419:Bacteria / Q000187:drug effects*; D001952:Bridged-Ring Compounds / Q000494:pharmacology; D016984:Enterococcus faecium / Q000187:drug effects; D004926:Escherichia coli / Q000187:drug effects; D012417:Rumen / Q000382:microbiology*; D012756:Sheep; D013210:Staphylococcus / Q000187:drug effects; D016985:Streptococcus bovis / Q000187:drug effects
D000900:Anti-Bacterial Agents; D001952:Bridged-Ring Compounds; C012306:enterocin