title: Cloning of chicken and mouse alpha 1b adrenergic receptor.
abstract: A partial cDNA encoding most of the third intracellular loop of the chicken alpha 1b adrenergic receptor subtype, obtained by reverse transcription-polymerase chain reaction (RT-PCR) techniques using degenerate primers derived from mammalian sequences, was used to isolate an alpha 1b adrenergic receptor cDNA from brain. The cDNA encodes a potential protein of 507 amino acids and Northern hybridization of poly(A)+ RNA from chicken brain of different developmental stages detected a single 3.5 kb transcript. Analysis of receptor expression indicated that the alpha 1b adrenergic receptor is widely distributed in chicken tissues, specially kidney and liver. cDNA and genomic clones encoding sequences of the mouse alpha 1b adrenergic receptor were also isolated.
authors.affiliation: Departamento de Bioquímica y Biología Molecular, Universidad de León, Spain.
authors.forename: A
authors.initials: A
authors.lastname: Alonso-Llamazares
authors.forename: J
authors.initials: J
authors.lastname: López-Alonso
authors.forename: M
authors.initials: M
authors.lastname: del Barrio
authors.forename: E
authors.initials: E
authors.lastname: Casanova
authors.forename: P
authors.initials: P
authors.lastname: Calvo
authors.forename: M A
authors.initials: MA
authors.lastname: Chinchetru
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D015152:Blotting, Northern; D001921:Brain; D002645:Chickens; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000302:isolation & purification; D051379:Mice; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D018340:Receptors, Adrenergic, alpha-1 / Q000235:genetics*; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid
chemical_list: C547717:Adra1b protein, mouse; D018076:DNA, Complementary; D018340:Receptors, Adrenergic, alpha-1