[DNA image cytometry in urothelial carcinomas. A pilot study of a new application module of the Lucia G DNA analysis system].
OBJECTIVE
To introduce and test a standardized measurement protocol for DNA image cytometry into a newly developed LUCIA G system application program. Contribution to a discussion concerning the possibility of using the histology sections for DNA image cytometry.


METHODS
Routine biopsy material from 18 urinary bladder cancer cases was analyzed (Grade 1: n = 6, Grade 2: n = 6, Grade 3: n = 6), both in histological and in cytoseparated preparations. More than one hundred diagnostic and more than 30 reference cells were measured. DNA features (mean DNA value, 2cDI, Böcking's grade of malignancy) and histogram types in Auer's classification (AI-IV) were compared.


RESULTS
Most carcinomas measured were aneuploid (two thirds G1, all G2, G3). The most common histogram type was A IV. Malignancy grade was mostly slightly higher in cytoseparated material, the shape of the histogram was analogous. Two carcinomas looking euploid in histology sections were proven aneuploid in cytoseparation.


CONCLUSIONS
More convenient tissue section measurement provided useful DNA profile information in most cases. Those without proven aneuploidy should be additionally measured in cytoseparations. An experienced pathologist meeting strictly measurement protocol demands is an important part of the measurement system.
Dusková
J
J
II. patologicko anatomický ústav 1. LF UK a katedra patologie IPVZ, Praha.
Mikes
J
J
Povýsil
C
C
D002295:Carcinoma, Transitional Cell / Q000235:genetics*; D004273:DNA, Neoplasm / Q000032:analysis*; D006801:Humans; D019044:Image Cytometry; D010865:Pilot Projects; D001749:Urinary Bladder Neoplasms / Q000235:genetics*
D004273:DNA, Neoplasm