Cloning of the ribosomal protein L41 gene of Phaffia rhodozyma and its use a drug resistance marker for transformation.
The ribosomal protein L41 gene of Phaffia rhodozyma was cloned and used as a dominant selectable marker for cycloheximide resistance in the transformation of P. rhodozyma. Electrotransformation with a plasmid containing a ribosomal DNA fragment as a targeting signal typically yielded 800 to 1,200 transformants/microgram of DNA with an integrated copy number of about seven per haploid genome.
Kim
I G
IG
Applied Microbiology Research Division, Korea Research Institute of Bioscience and Biotechnology, Yusong, Taejon, Korea.
Nam
S K
SK
Sohn
J H
JH
Rhee
S K
SK
AN
G H
GH
Lee
S H
SH
Choi
E S
ES
D003001:Cloning, Molecular; D003513:Cycloheximide / Q000494:pharmacology*; D004352:Drug Resistance, Microbial; D005800:Genes, Fungal; D010957:Plasmids; D016133:Polymerase Chain Reaction; D012269:Ribosomal Proteins / Q000235:genetics*; D014170:Transformation, Genetic; D015003:Yeasts / Q000187:drug effects / Q000235:genetics*
D012269:Ribosomal Proteins; D003513:Cycloheximide