Cloning and characterization of the genomic RNA sequence of the mumps virus strain associated with a high incidence of aseptic meningitis.
cDNA clones of the mumps virus wild-type strain, associated with a high incidence of aseptic meningitis (ODATE-1 strain), were isolated and analyzed from genomic nucleotide position 22 to 8520 containing the NP, P, M, F, SH and HN protein coding region. The ODATE-1 strain exhibited a RFLP profile identical to that of the Urabe vaccine strain in spite of the fact that the virus was isolated from non-vaccinated cases. However, a comparison of nucleotide and amino acid sequences among the ODATE-1 strain, Urabe strain and Miyahara strain revealed that the ODATE-1 strain was not related to the Urabe strain.
Saito
H
H
Department of Microbiology, Akita Prefectural Institute of Public Health, Japan.
Takahashi
Y
Y
Harata
S
S
Tanaka
K
K
Sato
H
H
Suto
T
T
Yamada
A
A
Yamazaki
S
S
Morita
M
M
D000818:Animals; D002522:Chlorocebus aethiops; D006801:Humans; D008582:Meningitis, Aseptic / Q000453:epidemiology / Q000821:virology*; D009109:Mumps virus / Q000235:genetics*; D012367:RNA, Viral / Q000737:chemistry*; D017423:Sequence Analysis, RNA; D017386:Sequence Homology, Amino Acid; D013045:Species Specificity; D014709:Vero Cells
D012367:RNA, Viral