A dot-ELISA test using monoclonal antibody-purified antigens for the diagnosis of paragonimiasis caused by Paragonimus heterotremus.
A dot enzyme-linked immunosorbent assay (dot-ELISA) using antigens purified by monoclonal antibody-affinity chromatography was developed for detecting antibodies to Paragonimus heterotremus in four groups of subjects. They consisted of 30 patients with P. heterotremus infection, 93 patients with other parasitic infections, 18 patients with pulmonary tuberculosis and 30 normal, healthy controls. Sensitivity, specificity, as well as positive and negative predictive values of the test were 100, 97, 88, and 100%, respectively.
Maleewong
W
W
Department of Parasitology, Faculty of Medicine, Khon Kaen University, Thailand. wancham@medlib2.kku.ac.th
Intapan
P M
PM
Wongkham
C
C
Pajongthanasaris
M
M
Morakote
N
N
Tapchaisri
P
P
Chaicumpa
W
W
D000818:Animals; D000911:Antibodies, Monoclonal; D000947:Antigens, Helminth; D016022:Case-Control Studies; D002846:Chromatography, Affinity; D003429:Cross Reactions; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D006801:Humans; D015151:Immunoblotting / Q000379:methods*; D010237:Paragonimiasis / Q000175:diagnosis*; D010238:Paragonimus / Q000145:classification / Q000276:immunology*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013785:Thailand
D000911:Antibodies, Monoclonal; D000947:Antigens, Helminth