title: Three populations of mouse lymph node dendritic cells with different origins and dynamics.
abstract: We have identified three distinct populations of mouse lymph node dendritic cells (DC) that differ in their capacity to uptake Ag delivered by different routes, and in their dynamics. The "I-DCs" are large cells that resemble the interdigitating cells and have a mature phenotype and a slow turnover. They derive from the regional tissues. The "sm-DCs" and "sI-DCs" are smaller (hence s-DC), have a more immature phenotype and a rapid turnover. The sI-DC phenotype, including CD8alpha expression suggests a lymphoid-related origin. The sI-DC population is expanded 100-fold after an in vivo flt3 ligand treatment. The sm-DC phenotype suggests a myeloid-related origin. Interestingly, sm-DCs can acquire i.v. injected macromolecules in less than 30 min after injection. They may, therefore, play an important role in the immune response against blood Ags.
authors.affiliation: Laboratoire de Biologie et Thérapeutique des Pathologies Immunitaires, Université Pierre et Marie Curie, Centre National de la Recherche Scientifique, Paris, France.
authors.forename: B
authors.initials: B
authors.lastname: Salomon
authors.forename: J L
authors.initials: JL
authors.lastname: Cohen
authors.forename: C
authors.initials: C
authors.lastname: Masurier
authors.forename: D
authors.initials: D
authors.lastname: Klatzmann
mesh_terms: D000818:Animals; D015415:Biomarkers / Q000097:blood; D018845:CD11 Antigens / Q000032:analysis; D002453:Cell Cycle / Q000276:immunology; D002454:Cell Differentiation / Q000276:immunology; D003713:Dendritic Cells / Q000166:cytology* / Q000276:immunology* / Q000378:metabolism; D003911:Dextrans / Q000008:administration & dosage / Q000276:immunology; D016650:Fluorescein-5-isothiocyanate / Q000008:administration & dosage; D006410:Hematopoiesis / Q000187:drug effects / Q000276:immunology; D000949:Histocompatibility Antigens Class II / Q000096:biosynthesis; D007274:Injections, Intraperitoneal; D007275:Injections, Intravenous; D008198:Lymph Nodes / Q000166:cytology* / Q000276:immunology / Q000378:metabolism; D046911:Macromolecular Substances; D008565:Membrane Proteins / Q000008:administration & dosage; D051379:Mice; D008810:Mice, Inbred C57BL; D008808:Mice, Inbred CBA; D008811:Mice, Inbred DBA; D008822:Mice, Transgenic; D012867:Skin / Q000276:immunology
chemical_list: D015415:Biomarkers; D018845:CD11 Antigens; D003911:Dextrans; D000949:Histocompatibility Antigens Class II; D046911:Macromolecular Substances; D008565:Membrane Proteins; C084881:flt3 ligand protein; D016650:Fluorescein-5-isothiocyanate