Modelling the infundibulum in acne.
The human sebaceous-pilosebaceous infundibulum was isolated and maintained for 7 days. Infundibular viability was maintained over 7 days in this medium. The addition of 1 ng/ml interleukin 1 alpha (IL-1 alpha) caused hypercornification of the infundibulum similar to that seen in comedones which could be blocked by 1,000 ng/ml IL-1 receptor antagonist. However, in contrast to interferon gamma 1, IL-1 alpha had no effect on the expression of intercellular adhesion molecule or human leucocyte antigen type DR in infundibular keratinocytes. The addition of 5 ng/ml epidermal growth factor or 5 ng/ml transforming growth factor alpha to the medium caused a disorganization of the keratinocytes of the infundibulum. We are now, therefore, able to model histologically the major infundibular changes in acne. Furthermore, we suggest that IL-1 alpha causes the scaling seen in many inflammatory skin diseases.
Guy
R
R
Department of Clinical Biochemistry, University of Cambridge, Addenbrooke's Hospital, UK.
Kealey
T
T
D015703:Antigens, CD; D000943:Antigens, Differentiation; D015815:Cell Adhesion Molecules / Q000378:metabolism; D046508:Culture Techniques; D004815:Epidermal Growth Factor / Q000494:pharmacology; D006684:HLA-DR Antigens / Q000378:metabolism; D018859:Hair Follicle / Q000378:metabolism / Q000473:pathology; D006801:Humans; D018799:Intercellular Adhesion Molecule-1 / Q000378:metabolism; D007371:Interferon-gamma / Q000494:pharmacology; D007375:Interleukin-1 / Q000494:pharmacology; D007633:Keratins / Q000378:metabolism; D012627:Sebaceous Glands / Q000166:cytology* / Q000378:metabolism*; D016211:Transforming Growth Factor alpha / Q000494:pharmacology
D015703:Antigens, CD; D000943:Antigens, Differentiation; D015815:Cell Adhesion Molecules; D006684:HLA-DR Antigens; C483616:ICAM3 protein, human; D007375:Interleukin-1; D016211:Transforming Growth Factor alpha; D018799:Intercellular Adhesion Molecule-1; D004815:Epidermal Growth Factor; D007633:Keratins; D007371:Interferon-gamma