title: An origin of transfer (oriT) on the conjugative element pRS01 from Lactococcus lactis subsp. lactis ML3.
abstract: Previous analysis of the Tra1 region of the conjugative element pRS01 from Lactococcus lactis subsp. lactis ML3 suggested that an origin of transfer (oriT) was present. Deletion derivatives of this cloned Tra1 region were assayed for mobilization in the presence of the wild-type pRS01 element in trans. The pRS01 oriT was localized to a 446-nucleotide segment in the intergenic region between open reading frames ltrD and ltrE. Sequence analysis of this region revealed a cluster of direct and inverted repeat structures characteristic of oriT regions associated with other conjugative systems.
authors.affiliation: Department of Microbiology, University of Minnesota, Minneapolis 55455-0312, USA.
authors.forename: D A
authors.initials: DA
authors.lastname: Mills
authors.forename: T G
authors.initials: TG
authors.lastname: Phister
authors.forename: G M
authors.initials: GM
authors.lastname: Dunny
authors.forename: L L
authors.initials: LL
authors.lastname: McKay
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D002874:Chromosome Mapping; D003227:Conjugation, Genetic; D004251:DNA Transposable Elements; D004269:DNA, Bacterial / Q000235:genetics; D004926:Escherichia coli / Q000235:genetics; D013294:Lactococcus lactis / Q000235:genetics*; D008969:Molecular Sequence Data; D012091:Repetitive Sequences, Nucleic Acid; D018741:Replication Origin; D017384:Sequence Deletion
chemical_list: D004251:DNA Transposable Elements; D004269:DNA, Bacterial