title: Calcium mobilization in isolated epithelial cells of the endolymphatic sac.
abstract: Endolymphatic sac epithelial cells were isolated from the endolymphatic sac of the guinea pig by enzymatic and mechanical dissociation. The intracellular free calcium ion concentrations ([Ca2+]i) of the isolated cells were determined using the Ca(2+)-sensitive dye fura-2. The isolated cells were classified into two types, i.e. light and dark cells. In the resting state, [Ca2+]i in the cells was variable in both types of cells. In the presence of 200 microM ATP, there was a rapid rise in [Ca2+]i. These findings suggest that endolymphatic sac epithelial cells may have receptor-mediated Ca channels which may play an important role for a nerve-mediated local feedback system of the endolymphatic sac to regulate homeostasis of endolymph volume, pressure and electrolyte balance.
authors.affiliation: Department of Otolaryngology, Hiroshima University School of Medicine, Japan.
authors.forename: S
authors.initials: S
authors.lastname: Arishige
authors.forename: M
authors.initials: M
authors.lastname: Takumida
authors.forename: K
authors.initials: K
authors.lastname: Yajin
mesh_terms: D000818:Animals; D002118:Calcium / Q000378:metabolism*; D002467:Cell Nucleus / Q000378:metabolism; D002469:Cell Separation; D003593:Cytoplasm / Q000378:metabolism; D004712:Endolymphatic Sac / Q000166:cytology / Q000378:metabolism*; D004847:Epithelial Cells / Q000378:metabolism; D005456:Fluorescent Dyes; D016257:Fura-2; D006168:Guinea Pigs; D066298:In Vitro Techniques; D008856:Microscopy, Fluorescence
chemical_list: D005456:Fluorescent Dyes; D002118:Calcium; D016257:Fura-2