Genotypic and phenotypic characterization of two Swedish isolates and two prototypic strains of Coxiella burnetii.
Two Swedish isolates of Coxiella burnetii and the two prototype strains of the species, Nine Mile and Priscilla, were characterized with regard to their multiplication and cytopathic effect on BGM cells and by PCR-based amplification of repetitive element DNA and the C. burnetii-specific plasmids QpH1 and QpRS. Moreover, 1330 bp of each 16S rRNA gene were sequence-determined. All four strains multiplied at virtually the same rate and displayed the same type of vacuoles in the BGM cells. Genetic homogeneity was observed inasmuch as the 16S rDNA sequences were identical and the strains showed identical PCR amplification patterns using primers specific to enterobacterial repetitive intragenic consensus DNA sequences. The two Swedish strains and the Priscilla strain also showed identical patterns after PCR amplification of repetitive extragenic palindromic DNA sequences, whereas the Nine Mile strain demonstrated a similar, but not identical pattern. Thus, the investigated strains demonstrated very similar phenotypic and genotypic characteristics. This finding is discussed in view of the very rare occurrence of domestic Q fever in Sweden.
Sjöstedt
A
A
Department of Microbiology, Defence Research Establishment, Umeå, Sweden. sjostedt@ume.foa.se
Göransson
I
I
Macellaro
A
A
Norlander
L
L
D000818:Animals; D002460:Cell Line; D016997:Coxiella burnetii / Q000235:genetics* / Q000254:growth & development*; D017931:DNA Primers; D014644:Genetic Variation; D005838:Genotype; D000882:Haplorhini; D008969:Molecular Sequence Data; D010641:Phenotype; D010957:Plasmids / Q000032:analysis; D016133:Polymerase Chain Reaction / Q000379:methods; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012091:Repetitive Sequences, Nucleic Acid / Q000235:genetics; D017422:Sequence Analysis, DNA; D013548:Sweden; D014617:Vacuoles
D017931:DNA Primers; D012336:RNA, Ribosomal, 16S