The effect of recombinant basic fibroblast growth factor (bFGF) in fibrin adhesive vehicle on the healing of ischaemic and normal incisional skin wounds.
Normal and ischaemic incisional wounds in rats were treated with recombinant human basic fibroblast growth factor (rbFGF) in fibrin adhesive vehicle. After 10 days of healing the maximum load*S and stress*S (S denotes correction for shrinkage) of ischaemic wounds were increased by 45% and 39%, respectively, after treatment with 20 micrograms rbFGF and by 67% and 56% after treatment with 60 micrograms rbFGF. After 20 days only 20 micrograms rbFGF had any effect and increased maximum load*S by 31% and energy at maximum (load*S, stress*S) by 40%-48%. In normal wounds 0.6-20 micrograms and 60 micrograms rbFGF decreased the strength parameters by 19%-34% and 49%-52%, respectively, after 10 days. After 20 days there was no negative influence but 60 micrograms rbFGF increased the biomechanical properties by 15%-24%. Treatment with the fibrin adhesive vehicle alone decreased the biomechanical properties of ischaemic wounds after 20 days, and of normal wounds after both 10 and 20 days. In conclusion, rbFGF can improve the healing of ischaemic wounds and may be used in the treatment of ischaemic wounds in patients, but it can have negative effects on normal wound healing.
Quirinia
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Department of Connective Tissue Biology, University of Aarhus, Denmark.
Viidik
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D000818:Animals; D001696:Biomechanical Phenomena; D062109:Dermatologic Surgical Procedures; D004305:Dose-Response Relationship, Drug; D015718:Fibrin Tissue Adhesive / Q000494:pharmacology*; D016222:Fibroblast Growth Factor 2 / Q000494:pharmacology*; D006801:Humans; D007511:Ischemia / Q000503:physiopathology*; D008297:Male; D051381:Rats; D017208:Rats, Wistar; D011994:Recombinant Proteins / Q000494:pharmacology; D012867:Skin / Q000098:blood supply; D013524:Surgical Flaps / Q000098:blood supply*; D013718:Tensile Strength; D014945:Wound Healing / Q000187:drug effects*; D014950:Wounds, Penetrating / Q000503:physiopathology*
D015718:Fibrin Tissue Adhesive; D011994:Recombinant Proteins; D016222:Fibroblast Growth Factor 2