Matrix metalloproteinase MMP-19 (RASI-1) is expressed on the surface of activated peripheral blood mononuclear cells and is detected as an autoantigen in rheumatoid arthritis.
In order to characterize the autoimmune response participating in the pathogenesis of rheumatoid arthritis (RA) a cDNA expression library constructed from mRNAs which had been isolated from the inflamed synovium of an RA patients was screened with autologous IgG autoantibodies. This led to the identification of gene rasi-1 which encodes a protein showing sequence identity with the zinc-binding matrix metalloproteinase MMP-19. MMP-19 is detected on the surface of activated PBMCs, TH1 lymphocytes, and Jurkat T lymphoma cells. It exhibits gelatinolytic activity and is recognized by autoantibodies in 26% and, respectively, 33% of sera collected from RA patients and systemic lupus erythematosus (SLE) patients. The novel autoantigen MMP-19 thus could play a role in the pathological processes participating in RA-associated joint tissue destruction.
Sedlacek
R
R
Faculty of Biology, University of Konstanz, Germany. Radek-Sedlacek@uni-konstanz.de
Mauch
S
S
Kolb
B
B
Schätzlein
C
C
Eibel
H
H
Peter
H H
HH
Schmitt
J
J
Krawinkel
U
U
D000595:Amino Acid Sequence; D000818:Animals; D001172:Arthritis, Rheumatoid / Q000097:blood / Q000276:immunology*; D001324:Autoantigens / Q000235:genetics / Q000276:immunology / Q000302:isolation & purification / Q000378:metabolism*; D016466:CHO Cells; D006224:Cricetinae; D018076:DNA, Complementary / Q000096:biosynthesis / Q000737:chemistry; D004274:DNA, Recombinant / Q000235:genetics / Q000378:metabolism; D005260:Female; D015971:Gene Expression Regulation, Enzymologic; D006801:Humans; D007963:Leukocytes, Mononuclear / Q000201:enzymology*; D053505:Matrix Metalloproteinases, Secreted; D008666:Metalloendopeptidases / Q000235:genetics / Q000276:immunology / Q000302:isolation & purification / Q000378:metabolism*; D017422:Sequence Analysis, DNA; D013601:T-Lymphocytes / Q000201:enzymology / Q000276:immunology
D001324:Autoantigens; D018076:DNA, Complementary; D004274:DNA, Recombinant; D053505:Matrix Metalloproteinases, Secreted; D008666:Metalloendopeptidases; C108362:matrix metalloproteinase 19