Ooplasmic injection of elongating spermatids for the treatment of non-obstructive azoospermia.
We applied the technique of ooplasmic elongating spermatid injection to the treatment of non-obstructive azoospermia. Mature oocytes were injected with elongating spermatids isolated from testicular biopsy material obtained from 13 non-obstructed azoospermic men. Seventy-three oocytes were successfully injected with elongating spermatids and were then cultured for 36 h. At 13 h post-injection 68 oocytes were found to be activated and 52 of them were fertilized. Forty-one 2- to 4-cell stage embryos developed from normally fertilized oocytes were transferred. At least two embryos were transferred to each female partner. Two pregnancies were achieved. Elongating spermatid injection may have a role in the treatment of non-obstructive azoospermia.
Sofikitis
N V
NV
Reproductive Physiology and IVF Center, Department of Urology, Tottori University School of Medicine, Yonago, Japan.
Yamamoto
Y
Y
Miyagawa
I
I
Mekras
G
G
Mio
Y
Y
Toda
T
T
Antypas
S
S
Kawamura
H
H
Kanakas
N
N
Antoniou
N
N
Loutradis
D
D
Mantzavinos
T
T
Kalianidis
K
K
Agapitos
E
E
D000328:Adult; D001706:Biopsy; D002467:Cell Nucleus; D003593:Cytoplasm; D004624:Embryo Transfer; D005260:Female; D005307:Fertilization in Vitro / Q000379:methods*; D006801:Humans; D008297:Male; D008845:Microinjections; D018613:Microscopy, Confocal; D009845:Oligospermia / Q000628:therapy*; D009865:Oocytes / Q000648:ultrastructure*; D011247:Pregnancy; D013087:Spermatids; D013737:Testis / Q000473:pathology