Effect of oxygen concentration in the gas atmosphere during in vitro insemination of bovine oocytes on the subsequent embryonic development in vitro.
In vitro matured bovine oocytes were co-incubated with sperm for 18 hr in a droplet of fertilization medium under a gas atmosphere of 5% CO2 with 5 or 20% O2. After removing the cumulus cells, they were fixed to examine their fertilization rate, or cultured for another 154 hr in a chemically defined medium under 5% O2 to determine their development to the blastocyst stage. There was no difference between the 5 and 20% O2 groups in the fertilization rate. However, the percentage of inseminated oocytes which developed to the blastocyst stage was higher when in vitro insemination was conducted under 5% O2 compared with that under 20% O2 (34.4 vs. 24.7%, P < 0.05).
Takahashi
Y
Y
Department of Clinical Sciences, Graduate School of Veterinary Medicine, Hokkaido University, Sapporo, Japan.
Kanagawa
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H
D000818:Animals; D001755:Blastocyst / Q000502:physiology*; D002417:Cattle; D005314:Embryonic and Fetal Development / Q000502:physiology*; D005260:Female; D005307:Fertilization in Vitro; D008297:Male; D009865:Oocytes / Q000187:drug effects / Q000502:physiology*; D010100:Oxygen / Q000494:pharmacology*; D013084:Sperm-Ovum Interactions / Q000187:drug effects / Q000502:physiology*; D013094:Spermatozoa / Q000502:physiology
D010100:Oxygen