Oxygen regulation of the ccoN gene encoding a component of the cbb3 oxidase in Rhodobacter sphaeroides 2.4.1T: involvement of the FnrL protein.
The ccoNOQP gene cluster of Rhodobacter sphaeroides 2.4.1T encodes a cbb3 cytochrome oxidase which is utilized in oxygen-limited conditions for aerobic respiration. The beta-galactosidase activity of a ccoN::lacZ transcriptional fusion was low under high (30%)-oxygen and anaerobic growth conditions. Maximal ccoN::lacZ expression was observed when the oxygen concentration was lowered to 2%. In an FnrL mutant, ccoN::lacZ expression was significantly lower than in the wild-type strain, suggesting that FnrL is a positive regulator of genes encoding the cbb3 oxidase.
Mouncey
N J
NJ
Department of Microbiology and Molecular Genetics, The University of Texas Health Science Center Medical School, Houston 77030, USA.
Kaplan
S
S
D000332:Aerobiosis; D000693:Anaerobiosis; D001426:Bacterial Proteins / Q000378:metabolism; D001483:Base Sequence; D003576:Electron Transport Complex IV / Q000096:biosynthesis* / Q000235:genetics; D004926:Escherichia coli / Q000235:genetics / Q000254:growth & development; D015964:Gene Expression Regulation, Bacterial / Q000187:drug effects / Q000502:physiology*; D005798:Genes, Bacterial; D005810:Multigene Family; D010100:Oxygen / Q000494:pharmacology; D010101:Oxygen Consumption; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis; D012242:Rhodobacter sphaeroides / Q000201:enzymology* / Q000235:genetics*; D015534:Trans-Activators; D014158:Transcription, Genetic; D001616:beta-Galactosidase / Q000096:biosynthesis
D001426:Bacterial Proteins; C096744:FnrL protein, Rhodobacter sphaeroides; D011993:Recombinant Fusion Proteins; D015534:Trans-Activators; D003576:Electron Transport Complex IV; D001616:beta-Galactosidase; D010100:Oxygen