title: Failure to demonstrate Chlamydia pneumoniae in symptomatic abdominal aortic aneurysms by a nested polymerase chain reaction (PCR).
abstract: OBJECTIVE
  To investigate whether Chlamydia pneumoniae is present in symptomatic abdominal aortic aneurysms (AAA).
  
  
  METHODS
  After optimisation of DNA extraction procedures an inhibitor-controlled nested polymerase chain reaction (PCR) amplifying fragments of the gene encoding the C. pneumoniae specific major outer membrane protein was performed on 124 wall-specimens from 20 patients with symptomatic AAA.
  
  
  RESULTS
  None of the specimens contained C. pneumoniae-specific DNA. Minor inhibition of the PCR was noticed especially in media specimens.
  
  
  CONCLUSIONS
  Using a sensitive and specific nested PCR, we were not able to detect C. pneumoniae in symptomatic AAA. The failure to detect C. pneumoniae in symptomatic AAA, combined with previously reported positive findings in atherosclerotic lesions, supports the hypothesis that AAA and atherosclerosis might be two different disease entities.
authors.affiliation: Department of Vascular Surgery, Viborg Hospital, Denmark.
authors.forename: J S
authors.initials: JS
authors.lastname: Lindholt
authors.forename: L
authors.initials: L
authors.lastname: Ostergård
authors.forename: E W
authors.initials: EW
authors.lastname: Henneberg
authors.forename: H
authors.initials: H
authors.lastname: Fasting
authors.forename: P
authors.initials: P
authors.lastname: Andersen
mesh_terms: D017544:Aortic Aneurysm, Abdominal / Q000382:microbiology*; D002690:Chlamydia Infections / Q000175:diagnosis*; D016993:Chlamydophila pneumoniae / Q000302:isolation & purification*; D004269:DNA, Bacterial / Q000032:analysis; D006801:Humans; D016133:Polymerase Chain Reaction; D012680:Sensitivity and Specificity
chemical_list: D004269:DNA, Bacterial