title: Functions of conserved motifs in the RNA-dependent RNA polymerase of a yeast double-stranded RNA virus.
abstract: At least eight conserved motifs are visible in the totivirus RNA-dependent RNA polymerase (RDRP). We have systematically altered each of these in the Saccharomyces cerevisiae double-stranded RNA virus ScVL1 by substituting the conserved motifs from a giardiavirus. The results help define the conserved regions of the RDRP involved in polymerase function and those essential for other reasons.
authors.affiliation: Department of Biological Sciences, SUNY-Buffalo, New York 14260, USA.
authors.forename: E
authors.initials: E
authors.lastname: Routhier
authors.forename: J A
authors.initials: JA
authors.lastname: Bruenn
mesh_terms: D000595:Amino Acid Sequence; D002213:Capsid / Q000235:genetics / Q000378:metabolism; D017124:Conserved Sequence; D008969:Molecular Sequence Data; D016296:Mutagenesis; D012324:RNA-Dependent RNA Polymerase / Q000235:genetics / Q000378:metabolism*; D012441:Saccharomyces cerevisiae / Q000821:virology*; D012526:Satellite Viruses / Q000502:physiology; D019196:Totiviridae / Q000201:enzymology*
chemical_list: D012324:RNA-Dependent RNA Polymerase