title: Histochemical mapping of NADPH-diaphorase in monkey and human eyes.
abstract: OBJECTIVE
  To localize nicotinamide adenine dinucleotide phosphate-diaphorase (NADPH-d) in normal human and monkey eyes.
  
  
  METHODS
  NADPH-d activity in ocular tissue sections was revealed by histochemical staining.
  
  
  RESULTS
  NADPH-d staining was found in the anterior and posterior ocular segment of both species. In the anterior segment, reaction product was present in the corneal epithelium and endothelium, iris dilator and sphincter, lens capsule, ciliary muscle, and non-pigmented epithelial cells and stroma of the ciliary processes. Staining density in the longitudinal ciliary muscle was higher than in the other ciliary muscle regions in the human; such regionalization was less apparent in the monkey. The trabecular meshwork stained minimally if at all in both species. In the posterior segment, staining was seen around choroidal blood vessels and choroidal nerves, and in the retinal pigment epithelial, photoreceptor, bipolar, inner plexiform and ganglion cell layers.
  
  
  CONCLUSIONS
  NADPH-d is widespread in human and monkey eyes, indicating significant and presumably functionally relevant NO production in the various ocular structures.
authors.affiliation: Department of Ophthalmology, University of British Columbia, Vancouver, Canada. zchen@org.ecc.ubc.ca
authors.forename: Z
authors.initials: Z
authors.lastname: Chen
authors.forename: Q
authors.initials: Q
authors.lastname: Gu
authors.forename: P L
authors.initials: PL
authors.lastname: Kaufman
authors.forename: M S
authors.initials: MS
authors.lastname: Cynader
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000818:Animals; D005123:Eye / Q000201:enzymology*; D005260:Female; D006651:Histocytochemistry; D006801:Humans; D008252:Macaca fascicularis; D008253:Macaca mulatta; D008297:Male; D008875:Middle Aged; D009252:NADPH Dehydrogenase / Q000032:analysis*; D012016:Reference Values
chemical_list: D009252:NADPH Dehydrogenase