title: Identification and functional characterization of a novel human protein highly related to the yeast dynamin-like GTPase Vps1p.
abstract: Dynamin proteins containing a GTPase domain, a pleckstrin homology motif and a proline-rich tail participate in receptor-mediated endocytosis in organisms ranging from insects to vertebrates. In addition, dynamin-related GTPases, such as the yeast Golgi protein Vps1p, which lack both the pleckstrin homology motif and the proline-rich region, participate in vesicular transport within the secretory pathway in lower eukaryotes. However, no data is available on the existence of Vps1p-like proteins in mammalian cells. In this study, we report the identification and characterization of a novel gene encoding a human dynamin-related protein, DRP1, displaying high similarity to the Golgi dynamin-like protein Vps1p from yeast and to a Caenorhabditis elegans protein deposited in the databank. These proteins are highly conserved in their N-terminal tripartite GTPase domain but lack the pleckstrin homology motif and proline-rich region. Northern blot analysis reveals that the DRP1 mRNA is detected at high levels in human muscle, heart, kidney and brain. Immunolocalization studies in Chinese hamster ovary (CHO) cells using an epitope-tagged form of DRP1 and confocal microscopy show that this protein is concentrated in a perinuclear region that labels with the endoplasmic reticulum marker DiOC6(3) and the Golgi marker C5-DMB-Cer. In addition, the localization of DRP1 is highly similar to the localization of the endoplasmic reticulum and cis-Golgi GTPase Rab1A, but not to the staining for the trans-Golgi GTPase Rab6. Furthermore, overexpression of a cDNA encoding a GTP binding site mutant of DRP1 (DRP1(K38E)) in CHO cells decreases the amount of a secreted luciferase reporter protein, whereas the overexpression of wild-type DRP1 increases the secretion of this marker. Together, these results constitute the first structural and functional characterization of a mammalian protein similar to the yeast dynamin-related GTPase Vps1p and indicate that the participation of these proteins in secretion has been conserved throughout evolution.
authors.affiliation: Gastroenterology Research Unit, St Mary's Hospital and Departments of Molecular Neuroscience and Biochemistry and Molecular Biology, Mayo Clinic, Rochester, MN 55905, USA.
authors.forename: M
authors.initials: M
authors.lastname: Imoto
authors.forename: I
authors.initials: I
authors.lastname: Tachibana
authors.forename: R
authors.initials: R
authors.lastname: Urrutia
mesh_terms: D000818:Animals; D001483:Base Sequence; D001692:Biological Transport / Q000235:genetics; D016466:CHO Cells / Q000737:chemistry / Q000378:metabolism / Q000502:physiology; D002352:Carrier Proteins / Q000235:genetics*; D003001:Cloning, Molecular; D006224:Cricetinae; D034281:Dynamins; D004721:Endoplasmic Reticulum / Q000737:chemistry; D000939:Epitopes / Q000032:analysis; D005656:Fungal Proteins / Q000235:genetics; D020558:GTP Phosphohydrolases / Q000235:genetics*; D019204:GTP-Binding Proteins / Q000235:genetics; D015870:Gene Expression / Q000502:physiology; D017930:Genes, Reporter; D006056:Golgi Apparatus / Q000737:chemistry; D006801:Humans; D008156:Luciferases / Q000235:genetics; D008322:Mammals; D008869:Microtubule-Associated Proteins; D024101:Mitochondrial Proteins; D008969:Molecular Sequence Data; D011506:Proteins / Q000032:analysis / Q000235:genetics* / Q000378:metabolism; D051381:Rats; D017386:Sequence Homology, Amino Acid; D033921:Vesicular Transport Proteins; D015003:Yeasts / Q000737:chemistry; D020761:rab1 GTP-Binding Proteins
chemical_list: D002352:Carrier Proteins; D000939:Epitopes; D005656:Fungal Proteins; D008869:Microtubule-Associated Proteins; D024101:Mitochondrial Proteins; D011506:Proteins; D033921:Vesicular Transport Proteins; D008156:Luciferases; D020558:GTP Phosphohydrolases; D019204:GTP-Binding Proteins; C064553:VPS1 protein, S cerevisiae; D020761:rab1 GTP-Binding Proteins; C110768:DNM1L protein, human; C519851:Dnm1l protein, rat; D034281:Dynamins