title: Intraepithelial infiltration by mast cells with both connective tissue-type and mucosal-type characteristics in gut, trachea, and kidneys of IL-9 transgenic mice.
abstract: IL-9 transgenic mice were analyzed for the presence of mast cells in different tissues. In these mice, increased mast cell infiltration was found in the gastric and intestinal epithelium as well as in the upper airways and kidney epithelium, but not in other organs, such as skin. IL-9 transgenic mast cells do not show signs of massive degranulation such as that found in IL-4 transgenic mice and are not involved in spontaneous pathologic changes. Gastric mast cells showed a phenotype related to connective-type mast cells, since they were stained by safranin, and strong expression of mouse mast cell protease-4 and -5 was found in this organ. However, they also expressed proteases related to the mucosal cell type, such as mouse mast cell protease-1 and -2. In vitro, although IL-9 by itself did not induce mast cell development from bone marrow progenitors, it strongly synergized with stem cell factor for the growth and differentiation of mast cells expressing the same protease pattern as that observed in IL-9 transgenic mice. Since constitutive stem cell factor expression was observed in vivo, and anti-c-Kit Abs inhibited IL-9 transgenic mastocytosis in the gut, this synergistic combination of factors is likely to be responsible for the mastocytosis observed in IL-9 transgenic mice. Taken together, these data demonstrate that IL-9 induces the in vivo amplification of a nonclassical mast cell subset with a mucosal localization but expressing proteases characteristic of both connective tissue-type and mucosal mast cells.
authors.affiliation: Department of Pathology, St-Luc University Hospital, University of Louvain, Brussels, Belgium.
authors.forename: C
authors.initials: C
authors.lastname: Godfraind
authors.forename: J
authors.initials: J
authors.lastname: Louahed
authors.forename: H
authors.initials: H
authors.lastname: Faulkner
authors.forename: A
authors.initials: A
authors.lastname: Vink
authors.forename: G
authors.initials: G
authors.lastname: Warnier
authors.forename: R
authors.initials: R
authors.lastname: Grencis
authors.forename: J C
authors.initials: JC
authors.lastname: Renauld
mesh_terms: D000818:Animals; D001483:Base Sequence; D003239:Connective Tissue Cells / Q000276:immunology*; D017931:DNA Primers / Q000235:genetics; D004064:Digestive System / Q000166:cytology / Q000276:immunology; D004847:Epithelial Cells / Q000276:immunology; D016906:Interleukin-9 / Q000235:genetics* / Q000502:physiology; D007668:Kidney / Q000166:cytology / Q000276:immunology; D008407:Mast Cells / Q000166:cytology* / Q000276:immunology*; D051379:Mice; D008822:Mice, Transgenic; D009092:Mucous Membrane / Q000166:cytology / Q000276:immunology; D010641:Phenotype; D016133:Polymerase Chain Reaction; D019089:Stem Cell Factor / Q000502:physiology; D014132:Trachea / Q000166:cytology / Q000276:immunology
chemical_list: D017931:DNA Primers; D016906:Interleukin-9; D019089:Stem Cell Factor