title: Clinical and microbiological effects of controlled-release local delivery of minocycline on periodontitis in dogs.
abstract: OBJECTIVE
  To evaluate the clinical and microbiological efficacy of minocycline in a subgingival local delivery system as an adjunct to tooth scaling and root planing in dogs with periodontal disease.
  
  
  METHODS
  Nine 4- to 7-year-old Beagles with periodontitis.
  
  
  METHODS
  After scaling of teeth and root planing, 2 treatment and 1 or 2 control sites were selected for each dog: treated sites (n = 18) received minocycline hydrochloride periodontal formulation and control sites (n = 12) received ointment base (no minocycline). Gingival crevicular fluid was collected at a baseline (prior to treatment) and at week 4. Clinical and microbiological effects were evaluated and compared among sites.
  
  
  RESULTS
  In minocycline-treated sites, clinical indices were significantly decreased at week 4, compared with those at baseline. Minocycline-treated sites were associated with a significant decrease in gingival crevicular fluid, probing depth, and bleeding on probing values, compared with those for control sites at week 4. Compared with that for control sites, total bacteria count in periodontal pockets of minocycline-treated sites had an obvious tendency to decrease by week 4. Proportions of Porphyromonas and Fusobacterium spp were significantly decreased at week 4, compared with proportions at control sites and with pretreatment (baseline) values.
  
  
  CONCLUSIONS
  When used as an adjunct to tooth scaling and root planing, minocycline periodontal formulation stimulated favorable clinical and antimicrobial responses.
authors.affiliation: Department of Pathology, School of Dentistry at Matsudo, Nihon University, Chiba-ken, Japan.
authors.forename: K
authors.initials: K
authors.lastname: Hayashi
authors.forename: K
authors.initials: K
authors.lastname: Takada
authors.forename: M
authors.initials: M
authors.lastname: Hirasawa
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000008:administration & dosage / Q000627:therapeutic use*; D003692:Delayed-Action Preparations; D004283:Dog Diseases; D004285:Dogs; D005673:Fusobacterium / Q000187:drug effects / Q000302:isolation & purification; D005881:Gingiva / Q000187:drug effects / Q000382:microbiology / Q000503:physiopathology; D008911:Minocycline / Q000008:administration & dosage / Q000627:therapeutic use*; D009824:Ointments; D010518:Periodontitis / Q000188:drug therapy / Q000382:microbiology / Q000662:veterinary*; D017861:Porphyromonas / Q000187:drug effects / Q000302:isolation & purification; D013291:Streptococcus / Q000187:drug effects / Q000302:isolation & purification; D013997:Time Factors
chemical_list: D000900:Anti-Bacterial Agents; D003692:Delayed-Action Preparations; D009824:Ointments; D008911:Minocycline