title: Characterization of the heterochromatic chromosome regions in sheep.
abstract: In order to elucidate the structural chromosome organization of the heterochromatic regions in sheep, we have used C-banding, silver-staining, sequential CDD technique and restriction endonuclease banding. By these banding techniques we obtained four fractions of repetitive DNA, the autosomal fractions A and B, the C fraction in the X chromosome, and the D fraction in the Y chromosome. Silver staining revealed active nucleolus organizer regions (NOR's) on the telomeric GC-rich areas of chromosomes 1, 2, 3, 4, and 25 which were digested with HaeIII restriction endonuclease.
authors.affiliation: Departamento de Zootecnia, Genética y Mejora Animal, Facultad de Veterinaria, Universidad de Extremadura, Cáceres, Spain.
authors.forename: J L
authors.initials: JL
authors.lastname: Fernández-García
authors.forename: M
authors.initials: M
authors.lastname: Martínez-Trancón
authors.forename: A
authors.initials: A
authors.lastname: Rabasco
authors.forename: J A
authors.initials: JA
authors.lastname: Padilla
mesh_terms: D000818:Animals; D002871:Chromosome Banding; D002875:Chromosomes / Q000737:chemistry*; D004262:DNA Restriction Enzymes; D005260:Female; D006570:Heterochromatin; D007621:Karyotyping; D008297:Male; D009697:Nucleolus Organizer Region; D012756:Sheep / Q000235:genetics*; D016622:Silver Staining; D013194:Staining and Labeling; D016615:Telomere; D014960:X Chromosome / Q000737:chemistry; D014998:Y Chromosome / Q000737:chemistry
chemical_list: D006570:Heterochromatin; D004262:DNA Restriction Enzymes