[Irregular temperature dependence of the efficiency of nucleic acids site-directed modification by oligonucleotide reagents producing reactive intermediates].
A 22-mer target DNA pTGCCTGGAGCTGCTTGATGCCC (I) was modified by a dodecadeoxyribonucleotide reagent GCATCAAGCAGCp[N(CH3)CH2RCl] (II) containing a 3'-terminal alkylating group (RCl = -C6H4N(CH3)(CH2CH2Cl). An anomalous, bell-shaped temperature dependence of the maximum extent of the modification was found. Based on the analysis of the kinetic scheme of the modification, a possible explanation for this experimental fact is given.
Amirkhanov
N V
NV
Novosibirsk Institute of Bioorganic Chemistry, Siberian Division, Russian Academy of Sciences, Novosibirsk, Russia.
Neronova
N V
NV
D000478:Alkylation; D018076:DNA, Complementary / Q000737:chemistry* / Q000235:genetics; D007700:Kinetics; D016297:Mutagenesis, Site-Directed; D009841:Oligonucleotides / Q000737:chemistry*; D013696:Temperature
D018076:DNA, Complementary; D009841:Oligonucleotides