title: Isolation and characteristics of yeasts able to grow at low concentrations of nutrients.
abstract: Seven oligotrophic yeasts, which can grow in a 10(4)-fold dilution of malt-yeast-glucose-peptone medium (10(-4) YM), were mainly isolated from soil. These yeasts belong to the Cryptococcaceae. When inoculated at about 10(2) cells/ml in 10(-4) YM, the isolates grew to 1.4 x 10(3)-2.4 x 10(5) cells/ml after 3 days. Some culture collection yeasts fell into three groups according to their growth characteristics in 10(-4) YM, one group showing characteristics of the oligotrophic yeasts. The half-saturation values of uptake by the five isolated oligotrophic yeasts for D-glucose, L-leucine and L-amino acids were 6.0-25.0, 1.7-43.3 and 3.5-21.6 microM, respectively. The oligotrophic yeasts suspended in 10 mM-phosphate buffer (pH 6.0) had high tolerances for starvation, and remained more than 15% viable after 90 days of starvation.
authors.affiliation: Department of Bioresource Science, Faculty of Agriculture, Kagawa University, Japan. kimura@ag.kagawa-u.ac.jp
authors.forename: Y
authors.initials: Y
authors.lastname: Kimura
authors.forename: Y
authors.initials: Y
authors.lastname: Nakano
authors.forename: K
authors.initials: K
authors.lastname: Fujita
authors.forename: S
authors.initials: S
authors.lastname: Miyabe
authors.forename: S
authors.initials: S
authors.lastname: Imasaka
authors.forename: Y
authors.initials: Y
authors.lastname: Ishikawa
authors.forename: M
authors.initials: M
authors.lastname: Sato
mesh_terms: D003470:Culture Media / Q000737:chemistry*; D003904:Mitosporic Fungi / Q000254:growth & development / Q000302:isolation & purification; D010944:Plants / Q000382:microbiology; D012988:Soil Microbiology; D014871:Water Microbiology; D015003:Yeasts / Q000254:growth & development* / Q000302:isolation & purification*
chemical_list: D003470:Culture Media