title: Cloning and expression of an iron-containing superoxide dismutase in the parasitic protist, Trichomonas vaginalis.
abstract: A superoxide dismutase (SOD) gene of the parasitic protist Trichomonas vaginalis was cloned, sequenced, expressed in Escherichia coli, and its gene product characterized. It is an iron-containing dimeric protein with a monomeric mass of 22,067 Da. Southern blots analyses suggested the presence of seven iron-containing (FeSOD) gene copies. Hydrophobic cluster analysis revealed some peculiarities in the 2D structure of the FeSOD from T. vaginalis and a strong structural conservation between prokaryotic and eukaryotic FeSODs. Phylogenetic reconstruction of the SOD sequences confirmed the dichotomy between FeSODs and manganese-containing SODs. FeSODs of protists appeared to group together with homologous proteobacterial enzymes suggesting a possible origin of eukaryotic FeSODs through an endosymbiotic event.
authors.affiliation: Laboratoire de Biologie des Protistes, UPRESA 6023 CNRS, Aubière, France. viscogliosi@cicsun.univ-bpclermont.fr
authors.forename: E
authors.initials: E
authors.lastname: Viscogliosi
authors.forename: P
authors.initials: P
authors.lastname: Delgado-Viscogliosi
authors.forename: D
authors.initials: D
authors.lastname: Gerbod
authors.forename: M
authors.initials: M
authors.lastname: Dauchez
authors.forename: S
authors.initials: S
authors.lastname: Gratepanche
authors.forename: A J
authors.initials: AJ
authors.lastname: Alix
authors.forename: D
authors.initials: D
authors.lastname: Dive
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D018628:Gene Dosage; D007501:Iron; D008969:Molecular Sequence Data; D010802:Phylogeny; D017433:Protein Structure, Secondary; D013482:Superoxide Dismutase / Q000737:chemistry / Q000235:genetics*; D014246:Trichomonas vaginalis / Q000201:enzymology* / Q000235:genetics
chemical_list: D007501:Iron; D013482:Superoxide Dismutase