Plasma-to-lumen clearance of para-aminohippurate can replace 51Cr EDTA clearance in the evaluation of intestinal mucosal injury.
Intestinal mucosal injury of various degrees occurs in many clinical situations and is initially evidenced by altered mucosal permeability. The latter may be assessed in animal models by determination of plasma-to-intestinal lumen clearance of specific molecules, usually chromated 51Cr EDTA. The purpose of this study was to evaluate the usage of para-aminohippurate (PHA) as a substitute for the commonly used radioactive material, i.e., 51Cr-EDTA, in the evaluation of intestinal mucosal injury. An isolated loop of ileum was created in rats and constantly perfused with warmed normal saline. Both renal pedicles were ligated. Either 51Cr-EDTA (18.5 Bq/kg) or PAH (58 mg/kg) was injected i.v. Fifteen-minute intestinal ischemia was produced by clamping the superior mesenteric artery immediately after the end of an equilibration period. The perfusate was collected for 10 min prior to the initiation of intestinal ischemia, during the last 10 min of ischemia, and during the following three 10-min periods of reperfusion. Blood samples were collected at the end of each collection period for the determination of either PAH or 51Cr-EDTA concentrations and the calculation of either PHA or 51Cr-EDTA plasma-to-lumen clearances. PAH and 51Cr-EDTA plasma-to-lumen clearances followed the same pattern in all five assessed periods with no statistical difference between the two. PAH plasma-to-lumen clearance is a feasible, reliable, and inexpensive method for the evaluation of ischemia/reperfusion injury to the intestinal mucosa. It can safely replace the commonly used method in animal models that utilizes radioactive materials such as 51Cr-EDTA.
Udassin
R
R
Department of Pediatric Surgery, Hadassah University Hospital Mount Scopus, Jerusalem, Israel.
Haskel
Y
Y
Seror
D
D
Welbourne
T C
TC
D000818:Animals; D002614:Chelating Agents / Q000493:pharmacokinetics; D002860:Chromium Radioisotopes; D004492:Edetic Acid / Q000493:pharmacokinetics; D005069:Evaluation Studies as Topic; D007413:Intestinal Mucosa / Q000098:blood supply / Q000378:metabolism*; D008297:Male; D011897:Random Allocation; D051381:Rats; D015427:Reperfusion Injury / Q000175:diagnosis*; D010130:p-Aminohippuric Acid / Q000493:pharmacokinetics
D002614:Chelating Agents; D002860:Chromium Radioisotopes; D004492:Edetic Acid; D010130:p-Aminohippuric Acid