Sonication-dependent electroporation of the erythromycin-producing bacterium Saccharopolyspora erythraea.
We report the development of an electrotransformation method applicable to all strains of Saccharopolyspora erythraea examined to date. Vegetatively grown mycelia were rendered electrocompetent by subjecting mycelial suspensions to ultrasound pulses. The protocol provides an alternative route for the introduction of DNA into filamentous microorganisms otherwise recalcitrant to transformation techniques.
Fitzgerald
N B
NB
Abbott Laboratories, North Chicago, Illinois 60064, USA.
English
R S
RS
Lampel
J S
JS
Vanden Boom
T J
TJ
D001435:Bacteriophages / Q000235:genetics; D004269:DNA, Bacterial / Q000235:genetics / Q000302:isolation & purification; D004274:DNA, Recombinant / Q000235:genetics / Q000302:isolation & purification; D004279:DNA, Viral / Q000008:administration & dosage / Q000235:genetics; D018274:Electroporation / Q000379:methods*; D004917:Erythromycin / Q000096:biosynthesis*; D004926:Escherichia coli / Q000235:genetics; D005822:Genetic Vectors; D010957:Plasmids / Q000235:genetics; D016930:Saccharopolyspora / Q000235:genetics* / Q000378:metabolism* / Q000821:virology; D013010:Sonication; D014170:Transformation, Genetic
D004269:DNA, Bacterial; D004274:DNA, Recombinant; D004279:DNA, Viral; D004917:Erythromycin