title: Post-transplantation lymphoproliferative disorder of donor origin in a sex-mismatched renal allograft as proven by chromosome in situ hybridization.
abstract: We report a case of post-transplantation B-cell lymphoproliferative disorder (PTLPD) associated with Epstein-Barr virus (EBV) that developed in a renal allograft 5 months after transplantation. The lesion had a histologic appearance of diffuse large B-cell lymphoma with monoclonality demonstrated by in situ hybridization (ISH) for kappa and lambda mRNA. Both the male donor and the female recipient were EBV seropositive. The lymphoid cells in this lesion was proven to be of donor origin by ISH for the human Y chromosome on the paraffin-embedded sections of the allograft. The recipient of the other kidney from the same donor did not have evidence of lymphoma, and the patient was also free from disease 2 years after surgical removal of the lymphoma This case is an unusual PTLPD of donor origin; the majority of such lesions in solid organ transplantations are of recipient origin. Our findings demonstrate that the origin of PTLPD can be documented in selected cases using ISH with probes to the Y chromosome.
authors.affiliation: Department of Pathology, The University of Hong Kong, Queen Mary Hospital, China.
authors.forename: A N
authors.initials: AN
authors.lastname: Cheung
authors.forename: A C
authors.initials: AC
authors.lastname: Chan
authors.forename: L P
authors.initials: LP
authors.lastname: Chung
authors.forename: T M
authors.initials: TM
authors.lastname: Chan
authors.forename: I K
authors.initials: IK
authors.lastname: Cheng
authors.forename: K W
authors.initials: KW
authors.lastname: Chan
mesh_terms: D000328:Adult; D018562:Disease Transmission, Infectious; D005260:Female; D004854:Herpesvirus 4, Human / Q000302:isolation & purification; D006801:Humans; D007147:Immunoglobulin Light Chains / Q000032:analysis; D007150:Immunohistochemistry; D017403:In Situ Hybridization; D016030:Kidney Transplantation / Q000009:adverse effects*; D016393:Lymphoma, B-Cell / Q000737:chemistry / Q000209:etiology* / Q000235:genetics; D008297:Male; D012367:RNA, Viral / Q000032:analysis; D014998:Y Chromosome
chemical_list: D007147:Immunoglobulin Light Chains; D012367:RNA, Viral