Cloning and characterization of the phenylalanyl-tRNA synthetase beta subunit gene from Candida albicans.
A Candida albicans expression library was constructed from RNA isolated from regenerating protoplasts. A 1.4-kb cDNA clone was used to isolate a genomic fragment. Sequence analysis revealed an open reading frame of 593 amino acids with an overall identity of 63.6% with the phenylalanyl-tRNA synthetase beta subunit (FRS1) of Saccharomyces cerevisiae. We named it CaFRS1. It is located in a single copy in chromosome R, SfiI fragment M. Its expression showed a decrease during the cell wall regeneration process in protoplasts of both yeast and mycelial cells of C. albicans, suggesting its requirement thereof in initial steps of the cell wall synthesis.
Marcilla
A
A
Secció Departamental de Microbiologia, Facultat de Farmacia, Universitat de Valencia, Burjassot, Valencia, Spain. Antonio.Marcilla@uv.es
Pallotti
C
C
Gomez-Lobo
M
M
Caballero
P
P
Valentin
E
E
Sentandreu
R
R
D000595:Amino Acid Sequence; D001483:Base Sequence; D015139:Blotting, Southern; D002176:Candida albicans / Q000235:genetics*; D002473:Cell Wall / Q000378:metabolism; D003001:Cloning, Molecular; D005800:Genes, Fungal; D008969:Molecular Sequence Data; D010652:Phenylalanine-tRNA Ligase / Q000235:genetics* / Q000502:physiology
D010652:Phenylalanine-tRNA Ligase