Simultaneous determination of tetrahydrocortisol and tetrahydrocortisone in human plasma and urine by stable isotope dilution mass spectrometry.
A capillary gas chromatographic-mass spectrometric method for the simultaneous determination of tetrahydrocortisol (THF, 3alpha,11beta,17alpha,21-tetrahydroxy-5beta-preg nane-20-one), allo-tetrahydrocortisol (allo-THF, 3alpha,11beta,17alpha,21-tetrahydroxy-5alpha-pre gnane-20-one) and tetrahydrocortisone (THE, 3alpha,17alpha,21-trihydroxy-5beta-pregnane-11,20-dion e) in human plasma and urine is described. [1,2,3,4,5-2H5]THF (THF-d5), allo-[1,2,3,4,5-2H5]THF (allo-THF-d5) and [1,2,3,4,5-2H5]THE (THE-d5) were used as internal standards. A double derivatization (bismethylenedioxypentafluoropropionate, BMD-PFP) made possible the separation of the three tetrahydrocorticoids with good gas chromatographic behavior. Quantitation was carried out by selected-ion monitoring of the characteristic fragment ions ([M-30]+) of the BMD-PFP derivatives of THF, allo-THF and THE. The sensitivity, specificity, precision and accuracy of the method were demonstrated to be satisfactory for measuring low concentrations of THF, allo-THF and THE in human plasma and urine.
Furuta
T
T
Department of Medicinal Chemistry and Clinical Pharmacy, Tokyo University of Pharmacy and Life Science, Hachioji, Japan.
Namekawa
T
T
Shibasaki
H
H
Kasuya
Y
Y
D003903:Deuterium; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods*; D006801:Humans; D007201:Indicator Dilution Techniques; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013760:Tetrahydrocortisol / Q000032:analysis* / Q000097:blood / Q000737:chemistry / Q000652:urine; D013761:Tetrahydrocortisone / Q000032:analysis* / Q000097:blood / Q000737:chemistry / Q000652:urine
D013761:Tetrahydrocortisone; D013760:Tetrahydrocortisol; D003903:Deuterium