Thrombospondins in early Xenopus embryos: dynamic patterns of expression suggest diverse roles in nervous system, notochord, and muscle development.
The thrombospondins (TSPs) are a family of extracellular matrix (ECM) glycoproteins that modulate many cell behaviors including adhesion, migration, and proliferation. Here we report the molecular cloning of the Xenopus homologs of TSP-1 and TSP-3, and the developmental patterns of expression of Xenopus TSP-1, TSP-3, and TSP-4 mRNAs. Xenopus TSP-1 and TSP-3 protein sequences each share approximately 80% amino acid identity with their mammalian counterparts. TSP-1 mRNAs are detectable at low levels in fertilized eggs indicating that this TSP is a maternally deposited transcript. Zygotic expression of TSP-1, TSP-3, and TSP-4 begins at the end of gastrulation and transcripts encoding each protein accumulate through the tadpole stages of development. Whole mount in situ hybridizations reveal that each TSP mRNA is localized in the embryo with distinct, developmentally regulated patterns of expression. TSP-1 mRNAs are detected in a wide range of tissues including the floor plate of the neural tube, epidermis, somites, notochord and, most notably, alternating rhombomeres. Transcripts encoding TSP-3 are expressed in the notochord, floor plate, sensorial layer of the epidermis and sensory epithelia. TSP-4 mRNAs are restricted to somitic mesoderm and skeletal muscle. These data suggest that the TSPs represent a functionally diverse family of ECM proteins with tissue-specific functions during embryogenesis.
Urry
L A
LA
Mills College, Department of Biology, Oakland, California, USA.
Whittaker
C A
CA
Duquette
M
M
Lawler
J
J
DeSimone
D W
DW
D000595:Amino Acid Sequence; D000818:Animals; D003001:Cloning, Molecular; D004625:Embryo, Nonmammalian / Q000378:metabolism; D018507:Gene Expression Regulation, Developmental; D017403:In Situ Hybridization; D008969:Molecular Sequence Data; D009132:Muscles / Q000196:embryology / Q000378:metabolism*; D009420:Nervous System / Q000196:embryology / Q000378:metabolism*; D009672:Notochord / Q000378:metabolism*; D019699:Thrombospondins / Q000235:genetics* / Q000378:metabolism*; D013997:Time Factors; D014018:Tissue Distribution; D014982:Xenopus laevis
D019699:Thrombospondins