title: Efficient mutagenesis of zebrafish by a DNA cross-linking agent.
abstract: We developed a novel procedure for efficient mutagenesis of zebrafish using a DNA cross-linking agent 4,5',8-trimethylpsoralen (TMP), which is known to frequently induce small deletions in Escherichia coli and Caenorhabditis elegans. A specific-locus test and pilot screenings indicated that the TMP mutagenesis procedure was efficient. To confirm the successful mutagenesis by TMP, we characterized mutants with selective impairments in the nervous system. The no tectal neuron mutation hindered the development of the tectal neurons, while the edawakare mutation resulted in the enhancement of the extension and branching of the peripheral axons of trigeminal ganglion and Rohon-Beard sensory neurons. These results suggest that the TMP mutagenesis will provide an efficient method to isolate and characterize zebrafish mutants at molecular level.
authors.affiliation: Department of Molecular Neurobiology and Pharmacology, School of Medicine, University of Tokyo, Japan.
authors.forename: H
authors.initials: H
authors.lastname: Ando
authors.forename: M
authors.initials: M
authors.lastname: Mishina
mesh_terms: D000818:Animals; D017173:Caenorhabditis elegans / Q000235:genetics; D003432:Cross-Linking Reagents / Q000494:pharmacology*; D003433:Crosses, Genetic; D004247:DNA / Q000187:drug effects / Q000235:genetics; D004926:Escherichia coli / Q000235:genetics; D005260:Female; D008297:Male; D016296:Mutagenesis; D009153:Mutagens / Q000494:pharmacology*; D009474:Neurons / Q000187:drug effects* / Q000473:pathology; D009475:Neurons, Afferent / Q000166:cytology / Q000187:drug effects / Q000473:pathology; D013477:Superior Colliculi / Q000002:abnormalities*; D012668:Trigeminal Ganglion / Q000166:cytology / Q000187:drug effects / Q000473:pathology; D014307:Trioxsalen / Q000494:pharmacology*; D014404:Tubulin / Q000032:analysis; D015027:Zebrafish / Q000235:genetics*
chemical_list: D003432:Cross-Linking Reagents; D009153:Mutagens; D014404:Tubulin; D004247:DNA; D014307:Trioxsalen