An import signal in the cytosolic domain of the Neurospora mitochondrial outer membrane protein TOM22.
TOM22 is an integral component of the preprotein translocase of the mitochondrial outer membrane (TOM complex). The protein is anchored to the lipid bilayer by a central trans-membrane segment, thereby exposing the amino-terminal domain to the cytosol and the carboxyl-terminal portion to the intermembrane space. Here, we describe the sequence requirements for the targeting and correct insertion of Neurospora TOM22 into the outer membrane. The orientation of the protein is not influenced by the charges flanking its trans-membrane segment, in contrast to observations regarding proteins of other membranes. In vitro import studies utilizing TOM22 preproteins harboring deletions or mutations in the cytosolic domain revealed that the combination of the trans-membrane segment and intermembrane space domain of TOM22 is not sufficient to direct import into the outer membrane. In contrast, a short segment of the cytosolic domain was found to be essential for the import and assembly of TOM22. This sequence, a novel internal import signal for the outer membrane, carries a net positive charge. A mutant TOM22 in which the charge of the import signal was altered to -1 was imported less efficiently than the wild-type protein. Our data indicate that TOM22 contains physically separate import and membrane anchor sequences.
Rodriguez-Cousiño
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N
Adolf Butenandt Institut für Physiologische Chemie, Ludwig-Maximilians-Universität München, Goethestrasse 33, 80336 München, Germany.
Nargang
F E
FE
Baardman
R
R
Neupert
W
W
Lill
R
R
Court
D A
DA
D001692:Biological Transport; D002451:Cell Compartmentation; D003600:Cytosol; D004563:Electrochemistry; D008565:Membrane Proteins / Q000737:chemistry* / Q000378:metabolism* / Q000648:ultrastructure; D026901:Membrane Transport Proteins; D008928:Mitochondria / Q000378:metabolism* / Q000648:ultrastructure; D016297:Mutagenesis, Site-Directed; D009492:Neurospora crassa; D011956:Receptors, Cell Surface; D017384:Sequence Deletion; D013329:Structure-Activity Relationship
D008565:Membrane Proteins; D026901:Membrane Transport Proteins; D011956:Receptors, Cell Surface