title: Establishment and characterization of a porcine kidney cell line, FS-L3, which forms unique multicellular domes in serum-free culture.
abstract: A stable porcine kidney epithelial cell line, FS-L3, was established and maintained in Eagle's minimum essential medium containing 0.295% tryptose phosphate broth, 0.5% Bacto Peptone, and 10 mM N, N-Bis (2-hydroxyethyl)-2-aminoethanesulfonic acid without any serum. The mode of chromosomes is 37 to 38. The FS-L3 cells formed fluid-filled, multicellular, three-dimensional domes on a single monolayer. The number of domes increased markedly after further cultivation. The origin of this cell line was confirmed as porcine by hybridization using PRE-1, which can be detected as a specific sequence in the porcine genome. It was also found that FS-L3 cells were free from possible adventitious viruses and mycoplasmas.
authors.affiliation: Department of Exotic Diseases, National Institute of Animal Health, Tokyo, Japan.
authors.forename: Y
authors.initials: Y
authors.lastname: Sakoda
authors.forename: A
authors.initials: A
authors.lastname: Fukusho
mesh_terms: D000818:Animals; D002417:Cattle; D002455:Cell Division; D002460:Cell Line; D002522:Chlorocebus aethiops; D002875:Chromosomes; D006224:Cricetinae; D016895:Culture Media, Serum-Free; D007668:Kidney / Q000166:cytology*; D009174:Mycoplasma; D010576:Pestivirus; D012190:Retroviridae; D013552:Swine; D014709:Vero Cells
chemical_list: D016895:Culture Media, Serum-Free