Detection of HeLa cell contamination--presence of human papillomavirus 18 DNA as HeLa marker in JTC-3, OG and OE cell lines.
There are warnings of the contamination of cell cultures with HeLa cells in many laboratories in the world. The cell lines JTC-3, OG and OE that were established in Okayama in 1959, 1969 and 1971, respectively, were examined for human papillomavirus (HPV) 18 DNA by Southern blot hybridization. The HPV 18 DNA detected in these three cell lines showed hybridization patterns characteristic of the HPV 18 DNA in the HeLa cell line established in 1951. Southern hybridization patterns of HPV 18 DNA in the cellular DNA of the C4-II cervical cancer cell line that was established in the USA in 1962 was different from that of HeLa cells. These results suggest that the JTC-3, OG and OE cell lines have been contaminated by HeLa cells.
Ogura
H
H
Department of Microbiology, Okayama Prefecture Institute for Environmental Science and Public Health.
Fujii
R
R
Hamano
M
M
Kuzuya
M
M
Nakajima
H
H
Ohata
R
R
Mori
T
T
D015415:Biomarkers; D018929:Cell Culture Techniques / Q000379:methods*; D002460:Cell Line / Q000166:cytology / Q000821:virology; D004279:DNA, Viral / Q000302:isolation & purification*; D006367:HeLa Cells; D006801:Humans; D027383:Papillomaviridae / Q000302:isolation & purification*
D015415:Biomarkers; D004279:DNA, Viral