title: Cloning of cDNA encoding the common alpha subunit precursor molecule of pituitary glycoprotein hormones in the Australian lungfish, Neoceratodus forsteri.
abstract: We have isolated cDNA clones encoding a putative glycoprotein hormone alpha subunit precursor molecule from a pituitary cDNA library of the Australian lungfish (Neoceratodus forsteri) by a two-step plaque hybridization technique initially using cDNA encoding the toad glycoprotein hormone alpha subunit precursor molecule as the hybridization probe. The inserts (799 bp) of two of the isolated cDNA clones contained sequences of 5' and 3' untranslated regions, including a poly(A) stretch, and the entire coding sequence of the alpha subunit precursor molecule. The deduced amino acid sequence showed that its signal peptide consists of 24 residues and its mature protein 97 residues. In the mature protein, there is an insert of one residue (Ala) just after the 9th residue. This insert is unique to the Australian lungfish among vertebrate species studied. The amino acid sequence of the mature protein shares the common, or the same-group, amino acid residues at 9 positions with tetrapod and not actinopterygian vertebrates, while only one residue is common to some teleosts and the lungfish to the exclusion of the tetrapods. The overall sequence of the mature protein of the Australian lungfish also shares more similarity with those of tetrapods (69 to 84%) than it does with teleosts (57 to 74%). These results on the relation of the alpha subunit precursor molecule between the lungfish and other vertebrates are consistent with the recent molecular phylogenetic studies suggesting a closer relationship between lungfish and tetrapods than between lungfish and teleosts. We also found that the primary structure of the lungfish alpha subunit is slightly but significantly more similar to that of homeothermic vertebrates than to that of amphibians. This may be due to specialization of the amphibian alpha subunit molecules.
authors.affiliation: Department of Biology, School of Education, Waseda University, Tokyo, Japan. 696L5020@mn.waseda.ac.jp
authors.forename: Y
authors.initials: Y
authors.lastname: Arai
authors.forename: K
authors.initials: K
authors.lastname: Kubokawa
authors.forename: S
authors.initials: S
authors.lastname: Ishii
authors.forename: J M
authors.initials: JM
authors.lastname: Joss
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002025:Bufonidae; D003001:Cloning, Molecular; D004247:DNA / Q000096:biosynthesis*; D015342:DNA Probes; D005399:Fishes / Q000378:metabolism*; D006023:Glycoproteins / Q000096:biosynthesis*; D008297:Male; D008969:Molecular Sequence Data; D010907:Pituitary Hormones / Q000096:biosynthesis*; D016133:Polymerase Chain Reaction; D011498:Protein Precursors / Q000096:biosynthesis* / Q000737:chemistry
chemical_list: D015342:DNA Probes; D006023:Glycoproteins; D010907:Pituitary Hormones; D011498:Protein Precursors; D004247:DNA