title: Serum proteins with NAD+ glycohydrolase activity and anti-CD38 reactivity--elevated levels in serum of tumour patients.
abstract: NAD+ glycohydrolase activities in serum samples from cancer patients were two- to three-fold higher than the activities in samples from healthy controls. SDS-PAGE analysis of serum samples followed by Western blotting revealed the presence of two proteins of approximately 45 and approximately 21 kDa that were immunoreactive with human CD38-specific monoclonal antibodies T16, HIT2 and OKT 10. These proteins appeared to be more abundant in serum from cancer patients. NAD+ glycohydrolase activity in serum could be enriched by immunoaffinity chromatography by using T16-Sepharose 4B. The results suggest that the relative abundance of proteins immunologically related to CD38 may account for the elevated levels of glycohydrolase activities in serum of tumour patients.
authors.affiliation: Department of Biophysics, I.U. Istanbul Faculty of Medicine, Capa-Istanbul, Turkey.
authors.forename: C
authors.initials: C
authors.lastname: Korkut
authors.forename: L
authors.initials: L
authors.lastname: Yalçintepe
authors.forename: N
authors.initials: N
authors.lastname: Kiremit-Korkut
authors.forename: S
authors.initials: S
authors.lastname: Uzun-Altinöz
authors.forename: S
authors.initials: S
authors.lastname: Işsever
authors.forename: F
authors.initials: F
authors.lastname: Gümüşel
authors.forename: D
authors.initials: D
authors.lastname: Tiryaki
authors.forename: E
authors.initials: E
authors.lastname: Bermek
mesh_terms: D036541:ADP-ribosyl Cyclase; D051997:ADP-ribosyl Cyclase 1; D000293:Adolescent; D000328:Adult; D000368:Aged; D000911:Antibodies, Monoclonal / Q000494:pharmacology; D015703:Antigens, CD; D000943:Antigens, Differentiation / Q000276:immunology*; D001798:Blood Proteins / Q000032:analysis*; D005260:Female; D006801:Humans; D008297:Male; D008562:Membrane Glycoproteins; D008875:Middle Aged; D009244:NAD+ Nucleosidase / Q000097:blood* / Q000276:immunology*; D009369:Neoplasms / Q000097:blood*
chemical_list: D000911:Antibodies, Monoclonal; D015703:Antigens, CD; D000943:Antigens, Differentiation; D001798:Blood Proteins; D008562:Membrane Glycoproteins; D036541:ADP-ribosyl Cyclase; C498154:CD38 protein, human; D009244:NAD+ Nucleosidase; D051997:ADP-ribosyl Cyclase 1