title: Different apparent diffusion coefficient: water content correlations of gray and white matter during early ischemia.
abstract: OBJECTIVE
  Early and accurate diagnosis of brain edema in stroke patients is essential for the selection of appropriate treatment. We examined the correlations between the changes in the apparent diffusion coefficient (ADC), regional water content, and tissue ultrastructure during early focal cerebral ischemia.
  
  
  METHODS
  The left middle cerebral arteries of cats were occluded with an intramagnet occlusion/recirculation device. T2-weighted, diffusion-weighted, and perfusion imaging were performed repeatedly during the initial 3 hours after occlusion. The ADCs obtained from ADC maps were compared with the corresponding tissue water content values determined by gravimetry and electron microscopic water localization.
  
  
  RESULTS
  ADC reduction was detected in areas of low perfusion 15 minutes after occlusion and thereafter. The water content increase correlated linearly with the ADC decreases in both the gray and white matter. However, both the water content corresponding to an ADC value and the rate of ADC change of the gray and white matter differed significantly (P<.05) as follows: y = -10105x + 8533 (r=.86) and y = -6174x + 4611 (r=.67), respectively, where x is the water content (grams water per gram tissue) and y is the ADC (x 10(-6) mm2/s). Hydropic astrocytic swelling was seen in both structures, and in the white matter, oligodendroglial and myelinated axonal swelling and periaxonal space enlargement were observed.
  
  
  CONCLUSIONS
  When early ischemic edema in experimental focal cerebral ischemia is evaluated with ADC mapping, the different slopes and intercepts of the water content and ADC correlation lines for the gray and white matter, which probably reflect different ultrastructural localization of water, should be taken into account.
authors.affiliation: Department of Neuropathology, Medical Research Institute, Tokyo Medical and Dental University, Japan. t.kuroiwa.npat@mri1.mri.tmd.ac.jp
authors.forename: T
authors.initials: T
authors.lastname: Kuroiwa
authors.forename: T
authors.initials: T
authors.lastname: Nagaoka
authors.forename: M
authors.initials: M
authors.lastname: Ueki
authors.forename: I
authors.initials: I
authors.lastname: Yamada
authors.forename: N
authors.initials: N
authors.lastname: Miyasaka
authors.forename: H
authors.initials: H
authors.lastname: Akimoto
mesh_terms: D000704:Analysis of Variance; D000818:Animals; D001834:Body Water / Q000378:metabolism*; D001921:Brain / Q000378:metabolism*; D002415:Cats; D004058:Diffusion; D002546:Ischemic Attack, Transient / Q000378:metabolism*; D008279:Magnetic Resonance Imaging; D008854:Microscopy, Electron