The p53 tumor suppressor inhibits transcription of the TATA-less mouse DP1 promoter.
Cell cycle progression is subject to several regulatory controls, of which the p53 protein plays a major role in growth arrest, subsequent to the detection of cellular aberrations. It is well documented that p53 has the ability to inhibit transcription driven by several promoters, possibly via distinct mechanisms. In this report, we show that expression of the cell cycle regulatory transcription factor DP1 is strongly inhibited by p53, at the level of transcription and probably through the basal TATA-less promoter. This inhibitory activity has a relative specificity for the DP1 promoter compared with the functionally related E2F1 promoter or unrelated promoters such as those of the transcription factor ATFa or the thymidine kinase gene. Inhibition of DP1 transcription has implications in one of the several possible mechanisms through which p53 induces cell cycle arrest.
Gopalkrishnan
R V
RV
Institut de Génétique et de Biologie Moléculaire et Cellulaire (CNRS/INSERM, University Louis Pasteur), F-67404 Illkirch Cedex C.U. de Strasbourg, France.
Lam
E W
EW
Kedinger
C
C
D016475:3T3 Cells; D000818:Animals; D001483:Base Sequence; D018797:Cell Cycle Proteins / Q000235:genetics*; D002999:Clone Cells; D015536:Down-Regulation / Q000528:radiation effects; D004742:Enhancer Elements, Genetic; D051379:Mice; D008969:Molecular Sequence Data; D011401:Promoter Regions, Genetic; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D016385:TATA Box; D050680:Transcription Factor DP1; D014157:Transcription Factors / Q000235:genetics*; D014158:Transcription, Genetic / Q000502:physiology*; D016159:Tumor Suppressor Protein p53 / Q000378:metabolism / Q000502:physiology*; D014466:Ultraviolet Rays
D018797:Cell Cycle Proteins; D012333:RNA, Messenger; C493736:Tfdp1 protein, mouse; D050680:Transcription Factor DP1; D014157:Transcription Factors; D016159:Tumor Suppressor Protein p53