title: Ooplasmic injection of elongating spermatids for the treatment of non-obstructive azoospermia.
abstract: We applied the technique of ooplasmic elongating spermatid injection to the treatment of non-obstructive azoospermia. Mature oocytes were injected with elongating spermatids isolated from testicular biopsy material obtained from 13 non-obstructed azoospermic men. Seventy-three oocytes were successfully injected with elongating spermatids and were then cultured for 36 h. At 13 h post-injection 68 oocytes were found to be activated and 52 of them were fertilized. Forty-one 2- to 4-cell stage embryos developed from normally fertilized oocytes were transferred. At least two embryos were transferred to each female partner. Two pregnancies were achieved. Elongating spermatid injection may have a role in the treatment of non-obstructive azoospermia.
authors.affiliation: Reproductive Physiology and IVF Center, Department of Urology, Tottori University School of Medicine, Yonago, Japan.
authors.forename: N V
authors.initials: NV
authors.lastname: Sofikitis
authors.forename: Y
authors.initials: Y
authors.lastname: Yamamoto
authors.forename: I
authors.initials: I
authors.lastname: Miyagawa
authors.forename: G
authors.initials: G
authors.lastname: Mekras
authors.forename: Y
authors.initials: Y
authors.lastname: Mio
authors.forename: T
authors.initials: T
authors.lastname: Toda
authors.forename: S
authors.initials: S
authors.lastname: Antypas
authors.forename: H
authors.initials: H
authors.lastname: Kawamura
authors.forename: N
authors.initials: N
authors.lastname: Kanakas
authors.forename: N
authors.initials: N
authors.lastname: Antoniou
authors.forename: D
authors.initials: D
authors.lastname: Loutradis
authors.forename: T
authors.initials: T
authors.lastname: Mantzavinos
authors.forename: K
authors.initials: K
authors.lastname: Kalianidis
authors.forename: E
authors.initials: E
authors.lastname: Agapitos
mesh_terms: D000328:Adult; D001706:Biopsy; D002467:Cell Nucleus; D003593:Cytoplasm; D004624:Embryo Transfer; D005260:Female; D005307:Fertilization in Vitro / Q000379:methods*; D006801:Humans; D008297:Male; D008845:Microinjections; D018613:Microscopy, Confocal; D009845:Oligospermia / Q000628:therapy*; D009865:Oocytes / Q000648:ultrastructure*; D011247:Pregnancy; D013087:Spermatids; D013737:Testis / Q000473:pathology