title: Tactile hyperesthesia, altered epidermal innervation and plantar nerve injury in the hindfeet of rats housed on wire grates.
abstract: The effects of wire grates on nerve injury and recovery were examined in rats housed in cages with sawdust-covered solid flooring. For the first 3 weeks of the study, 20 rats were housed on sawdust alone and 20 rats were housed in cages with wire grates placed over the sawdust. For the remaining 9 weeks, 10 animals housed on sawdust had wire grates added to their cages, while grates were removed from the cages of 10 animals. The effects of tactile stimulation on hindpaw plantar skin was measured weekly using the Von Frey filament test. Intraepidermal innervation using PGP 9.5 immunostaining and plantar nerve histology were assessed at the end of the 12-week study. After just 1 week on grates, hindpaw withdrawal thresholds were already markedly decreased and remained low until the grates were removed at 3 weeks. Thresholds returned to normal by 4 weeks after removal of the grates. Wire grates also induced increases in PGP 9.5 immunoreactive intraepidermal fine nerve endings that were normalized after grate removal. Demyelination, Wallerian degeneration and Renaut bodies were induced in the medial plantar nerve in rats housed in cages with wire-grate flooring. Nerve injury was largely resolved after 9 weeks on sawdust flooring. These data demonstrate that wire grates rapidly induce hindpaw tactile hyperesthesia and plantar neuropathy in rats and emphasize a risk of using wire-grate cage flooring in studies assessing hindlimb function and structure.
authors.affiliation: Department of Pathology 0612, School of Medicine, University of California, 9500 Gilman Drive, La Jolla, San Diego, CA 92093-0612, USA. amizisin@ucsd.edu
authors.forename: A P
authors.initials: AP
authors.lastname: Mizisin
authors.forename: M W
authors.initials: MW
authors.lastname: Kalichman
authors.forename: R S
authors.initials: RS
authors.lastname: Garrett
authors.forename: K C
authors.initials: KC
authors.lastname: Dines
mesh_terms: D000818:Animals; D004817:Epidermis / Q000294:innervation*; D005260:Female; D005528:Foot / Q000294:innervation*; D006614:Hindlimb; D006799:Housing, Animal; D006941:Hyperesthesia / Q000503:physiopathology*; D007150:Immunohistochemistry; D009419:Nerve Tissue Proteins / Q000032:analysis; D059348:Peripheral Nerve Injuries; D011312:Pressure; D051381:Rats; D017207:Rats, Sprague-Dawley; D013869:Thiolester Hydrolases / Q000032:analysis; D014110:Touch / Q000502:physiology*; D043222:Ubiquitin Thiolesterase
chemical_list: D009419:Nerve Tissue Proteins; D013869:Thiolester Hydrolases; D043222:Ubiquitin Thiolesterase