Expression of large genomic clones in transgenic mice: new insights into apolipoprotein B structure, function and regulation.
Extensive manipulation of the apolipoprotein B gene in yeast and bacterial artificial chromosome clones and subsequent expression of these clones in transgenic mice have provided fresh insights into several aspects of apolipoprotein B biology, including the identification of sequences important for lipoprotein (a) assembly, the demonstration that intestinal expression of apolipoprotein B is controlled by DNA sequences > 50 kb from the gene, and the extraordinary finding that apolipoprotein B is expressed in the heart.
McCormick
S P
SP
Biochemistry Department, University of Otago, Dunedin, New Zealand. sally.mccormick@stonebow.otago.ac.nz
Nielsen
L B
LB
D000818:Animals; D001055:Apolipoproteins B / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D002876:Chromosomes, Bacterial; D003001:Cloning, Molecular; D003545:Cysteine / Q000737:chemistry; D004742:Enhancer Elements, Genetic; D005786:Gene Expression Regulation; D007413:Intestinal Mucosa / Q000378:metabolism; D017270:Lipoprotein(a) / Q000737:chemistry / Q000378:metabolism; D051379:Mice; D008822:Mice, Transgenic; D009206:Myocardium / Q000378:metabolism; D011938:Rec A Recombinases / Q000378:metabolism
D001055:Apolipoproteins B; D017270:Lipoprotein(a); D011938:Rec A Recombinases; D003545:Cysteine