Histone H2A.F/Z subfamily: the smallest member and the signature sequence.
The nucleotide sequence of a 700 basepair cDNA obtained from rabbit bladder was determined. It encodes a 123 amino acid protein, which is the smallest member of histone H2A.F/Z subfamily. The known H2A.F/Z variants are highly conserved in their central core regions of about 100 amino acids but more divergent in their N- and C-terminal ends. In addition to the seven amino acid signature sequence previously known for the H2A proteins, all the cloned H2A.F/Z variants contain an identical peptide sequence, L-E-Y-L-T-A-E-V-L-E-L-A-G-N-A. This 15 amino acid motif is proposed here as the signature sequence to identify new members of the H2A.F/Z subfamily.
Jiang
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W
Department of Biochemistry and Molecular Biology, Pennsylvania State University College of Medicine, Hershey 17033, USA. wjiang@psu.edu
Guo
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X
Bhavanandan
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VP
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D017124:Conserved Sequence / Q000235:genetics; D016208:Databases, Factual; D006657:Histones / Q000737:chemistry*; D008969:Molecular Sequence Data; D010802:Phylogeny; D012333:RNA, Messenger / Q000032:analysis; D011817:Rabbits; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D001743:Urinary Bladder / Q000737:chemistry
D006657:Histones; D012333:RNA, Messenger; C077020:histone H2A.F-Z