The DRPLA CAG repeats in an Italian population sample: evaluation of the polymorphism for forensic applications.
The DRPLA CAG repeats polymorphism has been studied in an Italian population sample. PCR amplification, manual PAGE and silver staining were employed. A total of 16 different alleles, spanning the range from 5 to 21 CAG triplettes, was observed. The heterozygosity was 0.81 and no significant deviation from Hardy-Weinberg equilibrium was found 81 meioses from parentage testing were also analyzed and a Mendelian pattern of inheritance was observed in all cases. In addition, we could successfully type DRPLA locus in some forensic specimens, 1 ng of DNA allowing clear definition of alleles. The authors conclude that the DRPLA CAG repeats analysis may be useful for forensic applications.
Pelotti
S
S
Department of Medicine and Public Health, University of Bologna, Italy.
Mantovani
V
V
Esposti
P D
PD
D'Apote
L
L
Bragliani
M
M
Maiolini
E
E
Abbondanza
A
A
Pappalardo
G
G
D000483:Alleles; D004247:DNA / Q000032:analysis*; D016172:DNA Fingerprinting / Q000379:methods; D017931:DNA Primers / Q000737:chemistry; D005260:Female; D006801:Humans; D007558:Italy; D008297:Male; D009419:Nerve Tissue Proteins / Q000235:genetics*; D019636:Neurodegenerative Diseases / Q000235:genetics*; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic; D018911:Trinucleotide Repeats / Q000235:genetics*
D017931:DNA Primers; D009419:Nerve Tissue Proteins; C095278:atrophin-1; D004247:DNA