title: Molecular cloning and expression of the canine metallothionein-III gene.
abstract: We have isolated and determined the complete nucleotide sequence of canine metallothionein-III (MT-III) cDNA. The predicted amino acid sequence of the canine MT-III showed a high homology (93%, 87% identity) to that of human and mouse MT-III. The canine MT-III had 2 insertions relative to known mammalian MT-I and MT-II: a threonine after the 4th amino acid and a block of 6 amino acids near the carboxyl terminus. Expression of the canine MT-III mRNA was found exclusively in the central nervous system, where neurons in the olfactory bulb, hippocampus and cerebral cortex showed predominant signals.
authors.affiliation: Department of Veterinary Pathology, Tottori University, Japan.
authors.forename: S
authors.initials: S
authors.lastname: Kojima
authors.forename: A
authors.initials: A
authors.lastname: Shimada
authors.forename: A
authors.initials: A
authors.lastname: Kodan
authors.forename: K
authors.initials: K
authors.lastname: Kobayashi
authors.forename: T
authors.initials: T
authors.lastname: Morita
authors.forename: Y
authors.initials: Y
authors.lastname: Yamano
authors.forename: T
authors.initials: T
authors.lastname: Umemura
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D001921:Brain / Q000378:metabolism*; D004251:DNA Transposable Elements; D004285:Dogs; D006801:Humans; D008322:Mammals; D008668:Metallothionein / Q000096:biosynthesis* / Q000737:chemistry / Q000235:genetics*; D051379:Mice; D008969:Molecular Sequence Data; D009474:Neurons / Q000378:metabolism*; D009928:Organ Specificity; D012333:RNA, Messenger / Q000032:analysis; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000737:chemistry; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid
chemical_list: D004251:DNA Transposable Elements; D012333:RNA, Messenger; D011994:Recombinant Proteins; D008668:Metallothionein