Comparison between enzyme-linked immunosorbent assay, indirect fluorescent antibody and rapid conglutination tests in detecting antibodies against Babesia bovis.
The performance of an enzyme-linked immunosorbent assay (ELISA), an indirect fluorescent antibody test (IFAT) and a rapid conglutination test (RCT) for the detection of antibodies against Babesia bovis, was evaluated with 462 cattle sera from Bahia State; Brazil. The results showed a concordance of 96.6% between the ELISA and IFAT, 90.5% between the ELISA and RCT, and 91.8% between the IFAT and RCT. Although the prevalence rates determined by ELISA (97.2%) and IFAT (96.8%) were higher than that indicated by the RCT (92.9%), performances of the three serological tests were very similar and characterized the region studied as enzootically stable to B. bovis.
Araújo
F R
FR
Universidade para o Desenvolvimento do Estado e da Região do Pantanal, Brazil.
Madruga
C R
CR
Leal
C R
CR
Schenk
M A
MA
Kessler
R H
RH
Marques
A P
AP
Lemaire
D C
DC
D000818:Animals; D000913:Antibodies, Protozoan / Q000097:blood*; D016793:Babesia bovis / Q000276:immunology*; D001404:Babesiosis / Q000175:diagnosis* / Q000453:epidemiology / Q000517:prevention & control; D001938:Brazil / Q000453:epidemiology; D002417:Cattle; D002418:Cattle Diseases / Q000175:diagnosis* / Q000453:epidemiology / Q000517:prevention & control; D003168:Complement Fixation Tests / Q000662:veterinary*; D004797:Enzyme-Linked Immunosorbent Assay / Q000662:veterinary*; D005260:Female; D019084:Fluorescent Antibody Technique, Indirect / Q000662:veterinary*; D015995:Prevalence; D012680:Sensitivity and Specificity
D000913:Antibodies, Protozoan