title: Cytokines and matrix metalloproteinases mRNA expression in archival human tissues from failed total hip arthroplasty using in situ hybridization and color video image analysis.
abstract: Reports on the histological and biochemical nature of periprosthetic fibrous/granulomatous tissue has, to date, been largely limited to frozen tissue sections. This study reports the cytokine and matrix metalloproteinase profiles found in periprosthetic interface tissues in THA which have failed due to aseptic loosening and in capsular tissues obtained at primary surgery. The study employs immunohistochemistry, in situ hybridization, and color video image analysis on formalin fixed and paraffin embedded sections.
authors.affiliation: Orthopaedic Research Laboratory, Prince of Wales Hospital, University of New South Wales, Sydney, Australia.
authors.forename: Y
authors.initials: Y
authors.lastname: Yu
authors.forename: W R
authors.initials: WR
authors.lastname: Walsh
authors.forename: D H
authors.initials: DH
authors.lastname: Sonnabend
authors.forename: J L
authors.initials: JL
authors.lastname: Yang
authors.forename: F
authors.initials: F
authors.lastname: Bonar
authors.forename: B
authors.initials: B
authors.lastname: Markovic
authors.forename: W
authors.initials: W
authors.lastname: Bruce
authors.forename: L
authors.initials: L
authors.lastname: Kohan
authors.forename: M
authors.initials: M
authors.lastname: Neil
mesh_terms: D000368:Aged; D000369:Aged, 80 and over; D019644:Arthroplasty, Replacement, Hip; D016207:Cytokines / Q000032:analysis*; D015342:DNA Probes; D004790:Enzyme Induction; D005260:Female; D006097:Granulation Tissue / Q000737:chemistry*; D006801:Humans; D007150:Immunohistochemistry; D017403:In Situ Hybridization; D008297:Male; D019278:Matrix Metalloproteinase 3 / Q000032:analysis*; D008875:Middle Aged; D010003:Osteoarthritis / Q000601:surgery; D011475:Prosthesis Failure; D012333:RNA, Messenger / Q000032:analysis*
chemical_list: D016207:Cytokines; D015342:DNA Probes; D012333:RNA, Messenger; D019278:Matrix Metalloproteinase 3