title: Does acute exercise affect the susceptibility of low density lipoprotein to oxidation?
abstract: This study describes the effect of an acute exercise bout on the susceptibility of isolated low density lipoprotein (LDL) to in vitro oxidation. LDL was isolated from 23 subjects (exercisers, n = 11; sedentary, n = 12) immediately before and after a single bout of exercise (30 min of treadmill work at 55% & 70% peak oxygen consumption (VO2 peak) for exercisers and sedentary, respectively). A statistically significant decrease in lag time for LDL oxidation was observed following exercise compared to baseline (96.1+/-23.5 min vs. 92.1+/-23.3 minutes; n = 23, p < or = .03) using a 5 microM copper system. There was a statistically significant increase in plasma myeloperoxidase (MPO) levels following exercise compared to baseline values ( 1.58+/-.91 ng/dl versus 2.08+/-1.2 ng/dl; n = 12, p < or = .03). These results suggest that the 30 min exercise bout at a moderate intensity and duration was a sufficient oxidative stress to increase the susceptibility of LDL to in vitro oxidation. Additionally, the exercise bout appeared to activate neutrophils, subsequently releasing MPO protein.
authors.affiliation: Department of Nutrition & Food Science, Auburn University, AL, USA.
authors.forename: C J
authors.initials: CJ
authors.lastname: Wetzstein
authors.forename: R A
authors.initials: RA
authors.lastname: Shern-Brewer
authors.forename: N
authors.initials: N
authors.lastname: Santanam
authors.forename: N R
authors.initials: NR
authors.lastname: Green
authors.forename: J E
authors.initials: JE
authors.lastname: White-Welkley
authors.forename: S
authors.initials: S
authors.lastname: Parthasarathy
mesh_terms: D000328:Adult; D015444:Exercise / Q000502:physiology*; D005260:Female; D006801:Humans; D007700:Kinetics; D008077:Lipoproteins, LDL / Q000097:blood*; D008297:Male; D009504:Neutrophils / Q000502:physiology; D010084:Oxidation-Reduction; D010101:Oxygen Consumption; D009195:Peroxidase / Q000097:blood
chemical_list: D008077:Lipoproteins, LDL; C099252:oxidized low density lipoprotein; D009195:Peroxidase