Diagnosis of dengue by using reverse transcriptase-polymerase chain reaction.
A rapid identification of dengue viruses from clinical samples by using a nested reverse transcriptase-polymerase chain reaction (RT-PCR) procedure was carried out for diagnostic and epidemiological purposes. RT-PCR identified DEN-1 and DEN-2 viruses in 41% (41/100) of previously confirmed cases and provided an accurate confirmation of DHF in four fatal cases. RT-PCR was also useful for detecting and typing dengue viruses in suspected cases, allowing a rapid identification of new serotypes in endemic areas.
Miagostovich
M P
MP
Departamento de Virologia, Instituto Oswaldo Cruz, RJ, Brasil. marizepm@gene.dbbm.fiocruz.br
dos Santos
F B
FB
de Araújo
E S
ES
Dias
J
J
Schatzmayr
H G
HG
Nogueira
R M
RM
D001938:Brazil / Q000453:epidemiology; D003715:Dengue / Q000175:diagnosis* / Q000453:epidemiology; D003716:Dengue Virus / Q000302:isolation & purification*; D006801:Humans; D016133:Polymerase Chain Reaction / Q000379:methods*