DNA sequence similarity between California isolates of Cryptosporidium parvum.
We evaluated whether nucleic acid amplification with primers specific for Cryptosporidium parvum followed by automated DNA sequence analysis of the PCR amplicons could differentiate between California isolates of C. parvum obtained from livestock, humans, and feral pigs. Almost complete sequence identity existed among the livestock isolates and between the livestock and human isolates. DNA sequences from feral pig isolates differed from those from livestock and humans by 1.0 to 1.2%. The reference sequence obtained by Laxer et al. (M. A. Laxer, B. K. Timblin, and R. J. Patel, Am. J. Trop. Med. Hyg. 45:688-694, 1991.) differed from California isolates of C. parvum by 1.8 to 3.2%. These data suggest that DNA sequence analysis of the amplicon of Laxer et al. does not allow for differentiation between various strains of C. parvum or that our collection of isolates obtained from various hosts from across California was limited to one strain of C. parvum.
Pereira M das
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Veterinary Medicine Teaching and Research Center, School of Veterinary Medicine, University of California, Davis, Tulare 93274, USA.
Atwill
E R
ER
Crawford
M R
MR
Lefebvre
R B
RB
D000818:Animals; D000829:Animals, Domestic / Q000469:parasitology; D000835:Animals, Wild / Q000469:parasitology; D001483:Base Sequence; D002140:California; D002417:Cattle / Q000469:parasitology; D016785:Cryptosporidium parvum / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification*; D017931:DNA Primers / Q000235:genetics; D016054:DNA, Protozoan / Q000235:genetics* / Q000302:isolation & purification; D006041:Goats / Q000469:parasitology; D006736:Horses / Q000469:parasitology; D006801:Humans; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D012689:Sequence Homology, Nucleic Acid; D012722:Sewage / Q000469:parasitology; D013045:Species Specificity; D013552:Swine / Q000469:parasitology
D017931:DNA Primers; D016054:DNA, Protozoan; D012722:Sewage