Amperometric pH regulation--a flexible tool for rapid and precise temporal control over the pH of an electrolyte solution.
Temporal control over both pH and ionic strength of an electrolyte solution with high accuracy was achieved with a dynamic, computer feedback-controlled amperometric pH-stat device consisting of four pH-regulating electrodes placed in electrolyte reservoirs that are separated by dialysis membranes from a central compartment. Theoretical predictions of the behavior of this arrangement, obtained by computer simulation, were validated by running temporal pH programs such as step functions, oscillations, and linear pH gradients. Deviations from nominal values given by the computer program are within the limits of accuracy of the pH-measuring electrodes. No volume changes accompany a change of pH or conductivity since ions are forced to leave or enter the central compartment through the membranes by the electrical force applied between the pH-regulating electrodes. The device is flexible, easy to use and easily miniaturized. We discuss a wide range of possible applications in biochemistry and cell science. These include automated pH adjustment, isoelectric protein separation, amperometric measurement of enzyme kinetics and the response of cell cultures to well-defined pH changes.
Hagedorn
R
R
Mathematisch-Naturwissenschaftliche Fakultät I, Humboldt-Universität zu Berlin, Germany.
Korlach
J
J
Fuhr
G
G
D003198:Computer Simulation; D004573:Electrolytes; D019075:Electrophoresis, Capillary; D006863:Hydrogen-Ion Concentration; D009994:Osmolar Concentration; D015203:Reproducibility of Results; D012996:Solutions; D013997:Time Factors
D004573:Electrolytes; D012996:Solutions