Serum proteins with NAD+ glycohydrolase activity and anti-CD38 reactivity--elevated levels in serum of tumour patients.
NAD+ glycohydrolase activities in serum samples from cancer patients were two- to three-fold higher than the activities in samples from healthy controls. SDS-PAGE analysis of serum samples followed by Western blotting revealed the presence of two proteins of approximately 45 and approximately 21 kDa that were immunoreactive with human CD38-specific monoclonal antibodies T16, HIT2 and OKT 10. These proteins appeared to be more abundant in serum from cancer patients. NAD+ glycohydrolase activity in serum could be enriched by immunoaffinity chromatography by using T16-Sepharose 4B. The results suggest that the relative abundance of proteins immunologically related to CD38 may account for the elevated levels of glycohydrolase activities in serum of tumour patients.
Korkut
C
C
Department of Biophysics, I.U. Istanbul Faculty of Medicine, Capa-Istanbul, Turkey.
Yalçintepe
L
L
Kiremit-Korkut
N
N
Uzun-Altinöz
S
S
Işsever
S
S
Gümüşel
F
F
Tiryaki
D
D
Bermek
E
E
D036541:ADP-ribosyl Cyclase; D051997:ADP-ribosyl Cyclase 1; D000293:Adolescent; D000328:Adult; D000368:Aged; D000911:Antibodies, Monoclonal / Q000494:pharmacology; D015703:Antigens, CD; D000943:Antigens, Differentiation / Q000276:immunology*; D001798:Blood Proteins / Q000032:analysis*; D005260:Female; D006801:Humans; D008297:Male; D008562:Membrane Glycoproteins; D008875:Middle Aged; D009244:NAD+ Nucleosidase / Q000097:blood* / Q000276:immunology*; D009369:Neoplasms / Q000097:blood*
D000911:Antibodies, Monoclonal; D015703:Antigens, CD; D000943:Antigens, Differentiation; D001798:Blood Proteins; D008562:Membrane Glycoproteins; D036541:ADP-ribosyl Cyclase; C498154:CD38 protein, human; D009244:NAD+ Nucleosidase; D051997:ADP-ribosyl Cyclase 1