Rearrangement of c-myc gene in rapidly induced avian lymphoid leukosis tumors.
Southern blot hybridization of DNA samples from 9 primary tumors of avian lymphoid leukosis (LL) rapidly induced by ALV infection 27-74 days post inoculation was carried out to search for rearrangement of the c-myc gene with human c-myc gene exon III as a probe. Rearrangement of the c-myc gene was detected by appearance of new EcoRI fragments in 7 out of 9 tumors examined. The size of the fragments ranged from 3.1 to 4.0 kilobases (kb). In addition to these fragments, two fragments (9.0 kb and 13 kb) were observed in one tumor, and a faint fragment (3.5 kb) was observed in another tumor. Rearrangement of the c-myc gene was not detected in the remaining two tumors kept in unsuitable condition. These results suggest that rearrangement of c-myc gene was induced even in rapidly induced LL as well as that induced after long incubation period. This is the first report of involvement of c-myc gene in rapidly induced B-cell lymphoma (LL).
Hihara
H
H
National Institute of Animal Health, Ibaraki, Japan.
Nomura
N
N
Tsukamoto
K
K
Ishizaki
R
R
D000818:Animals; D001353:Avian Leukosis / Q000235:genetics*; D001354:Avian Leukosis Virus; D002645:Chickens; D015246:Deoxyribonuclease EcoRI; D005091:Exons; D015321:Gene Rearrangement; D016259:Genes, myc; D006801:Humans; D016393:Lymphoma, B-Cell / Q000235:genetics*; D015183:Restriction Mapping
D015246:Deoxyribonuclease EcoRI