Characterization of an 88-kDa heat shock protein of Neurospora crassa that interacts with Hsp30.
The small heat shock protein of Neurospora crassa, Hsp30, when employed in affinity chromatography, bound two cellular proteins that were identified as Hsp70 and Hsp88. Both Hsp70 and Hsp88 bound to Hsp30 in preference to other proteins, but binding of Hsp88 was more selective for Hsp30, and a direct interaction was observed. Transcripts for Hsp88, a newly characterized protein, are present at normal temperature, but they are strongly induced by heat shock. Its cDNA sequence predicts a protein with homology to mammalian Hsp110 family proteins, which are distantly related to Hsp70. Hsp88 and its homologues show greater similarity to Hsp70 in its N-terminal ATPase domain than in the C-terminal peptide-binding domain, and its ATP-binding motifs are conserved. Nevertheless, the N-terminal domain of Hsp88 (and related proteins) is consistently more hydrophobic and more basic than that of Hsp70 proteins. Within the C-terminal domain, the sequence corresponding to the DnaK alpha subdomain is conserved in the Hsp88/Hsp110 family proteins, whereas the DnaK beta subdomain sequence is not conserved. The interaction between Hsp70 and Hsp30 may reflect their cooperation as cochaperones for denatured proteins, whereas Hsp88 and Hsp30 may form a complex that interacts with potential substrates.
Plesofsky-Vig
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Department of Genetics and Cell Biology and of, The University of Minnesota, Saint Paul, Minnesota 55108, USA. nora@biosci.cbs.umn.edu
Brambl
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R
D000595:Amino Acid Sequence; D017124:Conserved Sequence; D005656:Fungal Proteins; D050890:HSP30 Heat-Shock Proteins; D006360:Heat-Shock Proteins / Q000737:chemistry / Q000378:metabolism*; D008565:Membrane Proteins / Q000378:metabolism*; D008969:Molecular Sequence Data; D009492:Neurospora crassa / Q000378:metabolism*; D011485:Protein Binding; D012331:RNA, Fungal; D011993:Recombinant Fusion Proteins / Q000378:metabolism
D005656:Fungal Proteins; D050890:HSP30 Heat-Shock Proteins; D006360:Heat-Shock Proteins; C112117:Hsp88 protein, Neurospora crassa; D008565:Membrane Proteins; D012331:RNA, Fungal; D011993:Recombinant Fusion Proteins