Induction of heme oxygenase-1 in toxic renal injury: mercuric chloride-induced acute renal failure in rat.
It is known that mercuric chloride (HgCl2) is a nephrotoxicant. When HgCl2 (1 mg/kg body weight) was intraperitoneally injected into rats, acute renal failure was induced. Histological changes in the kidneys were exclusively observed in the proximal tubules and the severe necrosis was found as early as 24 h after HgCl2 injection. The heme oxygenase-1 (HO-1) mRNA was strongly and promptly induced at about 2.5 h, the earliest time examined and abruptly decreased after the injection. Whereas the time course of HO-1 protein level was delayed as compared with that of HO-1 mRNA level. The levels of HO-1 mRNA and protein similarly increased with dose-dependent manner. The localization of HO-1 protein was restricted to the tubule cells. These findings suggest the potential involvement of HO-1 induction in the response to HgCl2-induced acute renal injury.
Horikawa
S
S
Department of Pathological Biochemistry, Medical Research Institute, Tokyo Medical and Dental University, Japan. hori-pbc@mri.tmd.ac.jp
Ito
K
K
Ikeda
S
S
Shibata
T
T
Ishizuka
S
S
Yano
T
T
Hagiwara
K
K
Ozasa
H
H
Katsuyama
I
I
D058186:Acute Kidney Injury / Q000139:chemically induced* / Q000662:veterinary; D000818:Animals; D006419:Heme Oxygenase (Decyclizing) / Q000096:biosynthesis* / Q000378:metabolism; D051547:Heme Oxygenase-1; D007668:Kidney / Q000187:drug effects / Q000473:pathology; D008297:Male; D008627:Mercuric Chloride / Q000633:toxicity*; D012333:RNA, Messenger / Q000096:biosynthesis; D051381:Rats; D017208:Rats, Wistar
D012333:RNA, Messenger; D008627:Mercuric Chloride; D006419:Heme Oxygenase (Decyclizing); D051547:Heme Oxygenase-1