title: Photoreaction of furocoumarins with DNA is similarly inhibited by minor and major groove-interacting ligands.
abstract: It was found that methyl green, a major groove binding ligand and the minor groove binding ligands, netropsin and 2,7-di-tert-butylproflavine inhibit, to a similar extend a monoadduct forming benzopsoralen and monoadduct and diadduct forming derivatives of psoralen (8-methoxypsoralen and 3,4'-dimethyl-8-methoxypsoralen). Caffeine exhibits an inhibitory effect on furocoumarin photobinding to DNA at 10(3) fold higher concentration. Together with the previously published results it is concluded that both occupancy of the major and minor groove as well as intercalation hinder photobinding of furocoumarins to DNA.
authors.affiliation: Instytut Fizjologii i Biochemii, Akademia Medyczna w Lodzi, Poland.
authors.forename: M
authors.initials: M
authors.lastname: Gniazdowski
authors.forename: Z
authors.initials: Z
authors.lastname: Tołwińska-Stańczyk
authors.forename: D
authors.initials: D
authors.lastname: Wilmańska
authors.forename: E
authors.initials: E
authors.lastname: Małagocka
mesh_terms: D000818:Animals; D002110:Caffeine / Q000737:chemistry; D002417:Cattle; D004247:DNA / Q000737:chemistry*; D008730:Methoxsalen / Q000031:analogs & derivatives* / Q000037:antagonists & inhibitors / Q000737:chemistry*; D008739:Methyl Green / Q000737:chemistry*; D009429:Netropsin / Q000737:chemistry*; D010777:Photochemistry; D011370:Proflavine / Q000031:analogs & derivatives* / Q000737:chemistry
chemical_list: C111823:2,7-di-tert-butylproflavine; D002110:Caffeine; D009429:Netropsin; D008739:Methyl Green; C067111:3,4'-dimethyl-8-methoxypsoralen; D004247:DNA; D011370:Proflavine; D008730:Methoxsalen