A satellite DNA element specific for roe deer (Capreolus capreolus).
An abundant tandem repetitive DNA segment (CCsatIII) with a repeat unit of 2.2 kb has been found in the genome of roe deer (Capreolus capreolus). It accounts for approximately 5%-10% of the genome and is only present in the two species of the genus Capreolus. The sequence has no similarity or common motives with other deer satellite DNAs and there is no internal repeat structure. A 93 bp fragment is homologous to a bovine repeat. Fluorescent in situ hybridisation showed a predominant centromeric staining of most chromosomes accompanied by a weak interstitial staining of the same chromosomes. On Southern blots, CCsatIII probes do not discriminate between the closely related Capreolus species.
Buntjer
J B
JB
Institute of Infectious Diseases and Immunology, Utrecht University, The Netherlands. j.buntjer@vetmic.dgk.ruu.nl
Nijman
I J
IJ
Zijlstra
C
C
Lenstra
J A
JA
D000818:Animals; D001483:Base Sequence; D002417:Cattle; D002503:Centromere / Q000235:genetics; D002874:Chromosome Mapping; D003001:Cloning, Molecular; D017931:DNA Primers / Q000235:genetics; D004276:DNA, Satellite / Q000235:genetics*; D003670:Deer / Q000235:genetics*; D015698:Genomic Library; D017404:In Situ Hybridization, Fluorescence; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D017422:Sequence Analysis, DNA; D013045:Species Specificity
D017931:DNA Primers; D004276:DNA, Satellite