Use of cyanoacrylate glue to prepare cultured keratinocyte sheets for grafting.
We describe a method to prepare keratinocyte cultured sheets for grafting based on the use of an organic glue (n-butyl-2-cyanoacrylate). It is a rapid and easy method in which the cultured layers of keratinocytes remain with a surface area similar to the original cultures.
Meana
A
A
Centro Comunitario de Transfusion del Principado de Asturias, Oviedo, Spain.
Iglesias
J
J
Madrigal
B
B
Sanchez
J
J
D002478:Cells, Cultured / Q000637:transplantation; D003487:Cyanoacrylates / Q000008:administration & dosage*; D006801:Humans; D015603:Keratinocytes / Q000166:cytology*; D016038:Skin Transplantation / Q000379:methods*
D003487:Cyanoacrylates