Hydroxyl radical formation resulting from the interaction of nitric oxide and hydrogen peroxide.
The highly reactive and cytotoxic hydroxyl radical (OH) was found by electrochemical detection to be produced in reactions involving hydrogen peroxide (H2O2) and the nitric oxide (NO) donor diethylamine- NO complex. Using aromatic hydroxylation of salicylate as a specific indicator of OH, three salicylate hydroxylation products were identified; catechol, 2,3- and 2,5-dihydroxybenzoic acid. Four additional compounds were detected but not identified. The interactions of H2O2 and NO represent a biologically feasible reaction mechanism that can account for OH-induced damage in cellular environments where transition metal ions are unavailable for participation in the superoxide-mediated Fenton reaction. The ability of the NO/H2O2 complex to generate OH independently of iron or other transition metals provides a new focus for studies concerned with the origin of tissue-specific damage caused by oxygen-derived species.
Nappi
A J
AJ
Department of Biology, Loyola University Chicago, IL 60626, USA. anappi@orion.it.luc.edu
Vass
E
E
D004563:Electrochemistry; D006861:Hydrogen Peroxide / Q000737:chemistry*; D017665:Hydroxyl Radical / Q000737:chemistry*; D006900:Hydroxylation; D066298:In Vitro Techniques; D007501:Iron / Q000737:chemistry; D009569:Nitric Oxide / Q000737:chemistry*; D010084:Oxidation-Reduction; D018384:Oxidative Stress; D017382:Reactive Oxygen Species; D012459:Salicylates / Q000737:chemistry; D020156:Salicylic Acid
D017382:Reactive Oxygen Species; D012459:Salicylates; D009569:Nitric Oxide; D017665:Hydroxyl Radical; D006861:Hydrogen Peroxide; D007501:Iron; D020156:Salicylic Acid