title: Comparison of three solid phase supports for promoting three-dimensional growth and function of human liver cell lines.
abstract: An extracorporeal liver support system will require that liver cells maintain their normal differentiated function. This is more likely to be achieved utilizing a three-dimensional culture configuration rather than a simple monolayer culture. We present data on a human liver cell line attached and maintained on different three-dimensional supports, porous glass (Siran), silicon (Immobasil), and calcium-alginate beads. Albumin, fibrinogen, prothrombin, alpha1-acid glycoprotein and alpha1-antitrypsin secretions were measured. Proliferation was slower on each of the three-dimensional supports than on the monolayer culture. The protein secretion of all 5 proteins was highest in cells encapsulated in alginate; silicon beads supported greater protein secretion than glass. Cells on silicon or within alginate were rounded; those on glass grew in 2 configurations as flattened monolayers and as rounded colonies. Cells in alginate secreted as much protein as the whole liver (e.g., albumin, 14.88 g/10(12) cells/day compared to the whole liver, approximately 12 g/day). Three-dimensional culture of a human liver cell line leads to both proliferation and a high synthetic capacity, an important feature of cells suitable for an extracorporeal liver support system.
authors.affiliation: Department of Histopathology, Imperial College School of Medicine, Hammersmith Hospital, London, England, UK.
authors.forename: C
authors.initials: C
authors.lastname: Selden
authors.forename: E
authors.initials: E
authors.lastname: Roberts
authors.forename: G
authors.initials: G
authors.lastname: Stamp
authors.forename: K
authors.initials: K
authors.lastname: Parker
authors.forename: P
authors.initials: P
authors.lastname: Winlove
authors.forename: T
authors.initials: T
authors.lastname: Ryder
authors.forename: H
authors.initials: H
authors.lastname: Platt
authors.forename: H
authors.initials: H
authors.lastname: Hodgson
mesh_terms: D000418:Albumins / Q000032:analysis; D000464:Alginates; D019149:Bioreactors; D002448:Cell Adhesion; D018929:Cell Culture Techniques / Q000379:methods*; D002455:Cell Division; D002460:Cell Line; D002470:Cell Survival; D005340:Fibrinogen / Q000032:analysis; D005898:Glass; D020723:Glucuronic Acid; D006603:Hexuronic Acids; D006801:Humans; D008099:Liver / Q000166:cytology* / Q000648:ultrastructure; D009961:Orosomucoid / Q000032:analysis; D011516:Prothrombin / Q000032:analysis; D012825:Silicon
chemical_list: D000418:Albumins; D000464:Alginates; D006603:Hexuronic Acids; D009961:Orosomucoid; D020723:Glucuronic Acid; D011516:Prothrombin; D005340:Fibrinogen; D012825:Silicon