title: Prenatal and postnatal diagnoses of thalassemias and hemoglobinopathies by HPLC.
abstract: The conventional approach to qualitative and quantitative analyses of hemoglobin (Hb) molecules for the diagnoses of hemoglobinopathies requires a combination of tests. We used an automated HPLC (VARIANT) system to study alpha-thalassemia and beta-thalassemia syndromes in Thailand. The beta-thalassemia short program is applicable to the diagnosis of alpha-thalassemia and beta-thalassemia disorders, including Hb H, EA Bart's disease, and EF Bart's disease, in adults, newborns, and fetuses. The system cannot quantify accurately certain Hb molecules, such as Hb H and Hb Bart's. The alpha-thalassemia short program was therefore developed and used to quantify Hb Bart's to detect alpha-thalassemia genotypes in cord blood. This automated HPLC system is an alternative approach to the diagnosis of complicated thalassemia syndromes in Thailand and Southeast Asia.
authors.affiliation: Thalassemia Research Center, Department of Medicine, Mahidol University, Nakornpathom, Thailand. grsfc@mahidol.ac.th
authors.forename: S
authors.initials: S
authors.lastname: Fucharoen
authors.forename: P
authors.initials: P
authors.lastname: Winichagoon
authors.forename: R
authors.initials: R
authors.lastname: Wisedpanichkij
authors.forename: B
authors.initials: B
authors.lastname: Sae-Ngow
authors.forename: R
authors.initials: R
authors.lastname: Sriphanich
authors.forename: W
authors.initials: W
authors.lastname: Oncoung
authors.forename: W
authors.initials: W
authors.lastname: Muangsapaya
authors.forename: J
authors.initials: J
authors.lastname: Chowthaworn
authors.forename: S
authors.initials: S
authors.lastname: Kanokpongsakdi
authors.forename: A
authors.initials: A
authors.lastname: Bunyaratvej
authors.forename: A
authors.initials: A
authors.lastname: Piankijagum
authors.forename: C
authors.initials: C
authors.lastname: Dewaele
mesh_terms: D000328:Adult; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D005260:Female; D005312:Fetal Blood / Q000737:chemistry; D006443:Hemoglobin A2 / Q000032:analysis; D006453:Hemoglobinopathies / Q000097:blood / Q000175:diagnosis*; D006455:Hemoglobins, Abnormal / Q000032:analysis; D006801:Humans; D007231:Infant, Newborn; D010641:Phenotype; D011247:Pregnancy; D011296:Prenatal Diagnosis / Q000379:methods*; D013785:Thailand; D017085:alpha-Thalassemia / Q000097:blood / Q000175:diagnosis*; D017086:beta-Thalassemia / Q000097:blood / Q000175:diagnosis*
chemical_list: D006455:Hemoglobins, Abnormal; D006443:Hemoglobin A2