title: Application of an optimized and highly discriminatory method based on arbitrarily primed PCR for epidemiologic analysis of methicillin-resistant Staphylococcus aureus nosocomial infections.
abstract: The optimization of an arbitrarily primed PCR method for typing 96 methicillin-resistant Staphylococcus aureus (MRSA) isolates was compared with pulsed-field gel electrophoresis. Identical results in the differentiation of MRSA clones and identification of the main cluster that included 82 strains (88% of patients) were obtained by both techniques.
authors.affiliation: Department of Microbiology, Hospital Dr. Peset, Valencia, Spain.
authors.forename: A
authors.initials: A
authors.lastname: Olmos
authors.forename: J J
authors.initials: JJ
authors.lastname: Camarena
authors.forename: J M
authors.initials: JM
authors.lastname: Nogueira
authors.forename: J C
authors.initials: JC
authors.lastname: Navarro
authors.forename: J
authors.initials: J
authors.lastname: Risen
authors.forename: R
authors.initials: R
authors.lastname: Sánchez
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D003428:Cross Infection / Q000175:diagnosis*; D004269:DNA, Bacterial / Q000032:analysis; D016521:Electrophoresis, Gel, Pulsed-Field; D005260:Female; D006801:Humans; D008297:Male; D016106:Methicillin Resistance; D008875:Middle Aged; D016133:Polymerase Chain Reaction; D013203:Staphylococcal Infections / Q000175:diagnosis*; D013211:Staphylococcus aureus / Q000187:drug effects / Q000235:genetics*
chemical_list: D004269:DNA, Bacterial