Cytotoxic action of 2-deoxy-D-glucose tetraacetate in tumoral pancreatic islet cells.
Tumoral insulin-producing cells of the RINm5F line were cultured for 8-96 h in the absence or presence of 2-deoxy-D-glucose (0.15-0.80 mM) or its tetraacetate ester (0.08-0.80 mM). Despite the fact that over a short incubation of 120 min the utilization of D-[5-3H]glucose and oxidation of D-[U-14C]glucose were not more markedly decreased by 2-deoxy-D-glucose tetraacetate than by the unesterified glucose analogue, the growth of the tumoral cells, as assessed by either the generation of formazan from 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide or direct cell counting, was more severely affected by the ester than by 2-deoxy-D-glucose. At a high concentration (0.80 mM), the ester even decreased the cell number below its initial value. No restoration of cell growth was observed when the cells were exposed for only 8 h to 2-deoxy-D-glucose tetraacetate (0.80 mM) and then further cultured for 64 h in the absence of the ester. These findings indicate that such an ester acts as a powerful cytostatic and cytotoxic agent in this tumoral cell line.
Malaisse
W J
WJ
Laboratory of Experimental Medicine, Brussels Free University, Belgium. malaisse@med.ulb.ac.be
Delvaux
A
A
Rasschaert
J
J
Kadiata
M M
MM
D007516:Adenoma, Islet Cell / Q000188:drug therapy* / Q000473:pathology; D000970:Antineoplastic Agents / Q000494:pharmacology*; D003847:Deoxyglucose / Q000494:pharmacology* / Q000627:therapeutic use; D010190:Pancreatic Neoplasms / Q000188:drug therapy* / Q000473:pathology; D014407:Tumor Cells, Cultured
D000970:Antineoplastic Agents; D003847:Deoxyglucose