A light and electron microscopic study of cytoplasmic phospholipase A2 and cyclooxygenase-2 in the hippocampus after kainate lesions.
The systemic administration of kainate (10 mg/ml) into adult Wistar rats produces seizures and neurodegeneration. We have studied the effect of kainate administration on cPLA2 and COX-2 immunoreactivities after 3 days and 1, 2, 4 and 11 weeks. The cPLA2 immunoreactivity was increased in hippocampal neurons at 1 and 3 days after kainate injection suggesting that PLA2 may be involved in neurodegeneration. Increased cPLA2 and COX-2 immunoreactivities in astrocytes at 1, 2, 4 and 11 weeks after kainate injection indicate an adaptive astrocytic response that may be associated with gliosis.
Sandhya
T L
TL
Department of Anatomy, National University of Singapore, Singapore 119260, Singapore.
Ong
W Y
WY
Horrocks
L A
LA
Farooqui
A A
AA
D000818:Animals; D051546:Cyclooxygenase 2; D003593:Cytoplasm / Q000201:enzymology*; D006624:Hippocampus / Q000201:enzymology*; D007150:Immunohistochemistry; D007275:Injections, Intravenous; D007527:Isoenzymes / Q000032:analysis*; D007608:Kainic Acid; D008297:Male; D008853:Microscopy / Q000379:methods; D008854:Microscopy, Electron; D009474:Neurons / Q000201:enzymology; D010544:Peroxidases / Q000032:analysis*; D010741:Phospholipases A / Q000032:analysis*; D054467:Phospholipases A2; D011451:Prostaglandin-Endoperoxide Synthases / Q000032:analysis*; D051381:Rats; D017208:Rats, Wistar
D007527:Isoenzymes; D010544:Peroxidases; D051546:Cyclooxygenase 2; D011451:Prostaglandin-Endoperoxide Synthases; D010741:Phospholipases A; D054467:Phospholipases A2; D007608:Kainic Acid