An approach to the study of primitive thrombocythemia (PT) megakaryocytes by means of Fourier transform infrared microspectroscopy (FT-IR-M).
Chronic myeloid leukemia (CML), polycythemia vera (PV), idiopathic myelofibrosis (IM) and primary thrombocythemia (PT) are myeloproliferative diseases of clonal origin. Megakaryocyte series are commonly involved in these disorders. In a previous paper of us, megakaryocytes (MKs) from PV and PT patients were shown to be more pathological with respect to the MKs from CML. This paper describes a Fourier transform infrared microspectroscopy (FT-IR-M) study analyzing the cytoplasm and nucleus areas of MKs from thrombocythemic patients which exhibited numerous giant cells (from 100 to 190 microm in diameter). The size of these cells makes it possible to analyze the cell parts using FT-IR-M technique. The infrared determinations on 10 single MKs for each case examined in these two different cell regions revealed spectral differences with a high degree of reproducibility. Finally, the spectra of whole MKs from normal donors and from thrombocythemic patients were also compared.
Benedetti
E
E
Department of Oncology, University of Pisa, Italy.
Bramanti
E
E
Papineschi
F
F
Vergamini
P
P
Benedetti
E
E
D006801:Humans; D008533:Megakaryocytes / Q000166:cytology*; D017550:Spectroscopy, Fourier Transform Infrared / Q000379:methods*; D013922:Thrombocytosis / Q000097:blood*