Mapping the genetic determinants of human immunodeficiency virus type 2 for cell tropism and replication efficiency.
Two distinct infectious molecular clones of human immunodeficiency type 2 (HIV-2) were analyzed for their biological properties in six cell lines. Fourteen chimeric and ten mutant viruses were constructed from these two viral genomes to localize the genetic determinants responsible for the phenotypes. Growth property of the viruses in the cell lines, together with the biochemical data, showed that a major determinant for the viral tropism resides in the env gene. In addition, in some cell lines, the accessory genes vif and nef affected the efficiency of virus replication. Thus, like HIV-1, mutations in the auxiliary and env genes of HIV-2 contributed much to the differences in virological characteristics.
Kawamura
M
M
Department of Virology, School of Medicine, University of Tokushima, Japan.
Shimano
R
R
Ogasawara
T
T
Inubushi
R
R
Amano
K
K
Akari
H
H
Adachi
A
A
D001483:Base Sequence; D002461:Cell Line, Transformed; D002874:Chromosome Mapping; D004279:DNA, Viral; D015696:Gene Products, tat / Q000378:metabolism; D015498:HIV-2 / Q000235:genetics* / Q000502:physiology*; D018922:HL-60 Cells; D006801:Humans; D008969:Molecular Sequence Data; D011995:Recombination, Genetic; D014407:Tumor Cells, Cultured; D014779:Virus Replication / Q000235:genetics*; D054322:tat Gene Products, Human Immunodeficiency Virus
D004279:DNA, Viral; D015696:Gene Products, tat; D054322:tat Gene Products, Human Immunodeficiency Virus