Significance of trisomy 7 related to PCNA index in cholesteatoma.
OBJECTIVE
To define the biological features of the variable aggressiveness of cholesteatoma, we performed a retrospective cytogenetic and kinetic study.


METHODS
We studied 44 cases of cholesteatoma by using fluorescence in situ hybridization with specific alpha-satellite DNA probes for chromosome 7 and immunohistochemical analysis of proliferating cell nuclear antigen (PCNA) with PC10 monoclonal antibody.


RESULTS
We observed the presence of three or four chromosome 7 in elevated percentages of cells of 23 cases, and a high PCNA index (above mean value) in 22 cases.


CONCLUSIONS
Based on our results, we maintain that the presence of an extra chromosome 7 is correlated with an increase of proliferative activity. Therefore, trisomy 7 can be considered a prognostic marker and an indicator of aggressive behavior in cholesteatoma.
Lavezzi
A
A
Institute of Pathology and the Department of Otorhinolaryngology, University of Milan, Italy.
Mantovani
M
M
Cazzulo
A
A
Turconi
P
P
Matturri
L
L
D000293:Adolescent; D000328:Adult; D015415:Biomarkers / Q000032:analysis; D018424:Cholesteatoma, Middle Ear / Q000235:genetics* / Q000378:metabolism; D002897:Chromosomes, Human, Pair 7; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D017404:In Situ Hybridization, Fluorescence; D008297:Male; D008875:Middle Aged; D018809:Proliferating Cell Nuclear Antigen / Q000032:analysis*; D012189:Retrospective Studies; D014314:Trisomy
D015415:Biomarkers; D018809:Proliferating Cell Nuclear Antigen