[Detection of hL6-mRNA: new possibilities in serologic tumor diagnosis of colorectal carcinomas].
A study was performed to detect circulating tumor cells in patients with colorectal cancer using mRNA coding for the tumor associated antigen L6. The mRNA was determined by Reverse Transcriptase Polymerase Chain Reaction and gel-electrophoresis. The L6 results were compared with the CEA levels. Peripheral blood samples were taken from 109 patients with histologically verified colorectal cancer. Statistics were carried out using CHI Square and Sokal and Rohlf's-test. Preoperatively 81.65% showed positive L6 mRNA, whereas only 58.7% had elevated CEA titers (p < 0.05). In all patients of the control group (n = 52) no L6 was detectable. Concerning our results L6 seems to be a sensitive and precise tool for diagnosing circulating tumor cells in colorectal cancer.
Schiedeck
T H
TH
Klinik für Chirurgie, Medizinische Universität zu Lübeck.
Christoph
S
S
Duchrow
M
M
Bruch
H P
HP
D000954:Antigens, Surface / Q000097:blood* / Q000235:genetics; D014408:Biomarkers, Tumor / Q000097:blood* / Q000235:genetics; D001797:Blood Protein Electrophoresis; D002872:Chromosome Deletion; D002887:Chromosomes, Human, Pair 18; D015179:Colorectal Neoplasms / Q000175:diagnosis* / Q000235:genetics / Q000601:surgery; D006801:Humans; D009363:Neoplasm Proteins / Q000097:blood* / Q000235:genetics; D009364:Neoplasm Recurrence, Local / Q000175:diagnosis / Q000235:genetics; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000097:blood* / Q000235:genetics; D012680:Sensitivity and Specificity
D000954:Antigens, Surface; D014408:Biomarkers, Tumor; D009363:Neoplasm Proteins; D012333:RNA, Messenger; C074075:TM4SF1 protein, human