Myocardial infarction induces expression of midkine, a heparin-binding growth factor with reparative activity.
We examined midkine (MK) expression in the rat heart upon experimental myocardial infarction. Immunohistochemical staining revealed, 6 hours after ligation of the left anterior descending coronary artery, strong MK immunoreactivity in myocytes and endothelial cells of a non-infarcted cardiac region. The myocytes of the infarcted cardiac region destined for death showed only a little immunoreactivity. Northern blot analysis suggested that the increased immunoreactivity was due to increased MK synthesis. The induced MK expression is likely to mimic the expression during embryogenesis: MK was strong lye-expressed in the myocytes of embryonic heart, and the expression decreased during embryogenesis.
Obama
H
H
Department of Biochemistry, Faculty of Medicine, Kagoshima University, Japan.
Biro
S
S
Tashiro
T
T
Tsutsui
J
J
Ozawa
M
M
Yoshida
H
H
Tanaka
H
H
Muramatsu
T
T
D000818:Animals; D002352:Carrier Proteins / Q000378:metabolism*; D016207:Cytokines; D015870:Gene Expression; D005865:Gestational Age; D006133:Growth Substances / Q000378:metabolism*; D006321:Heart / Q000196:embryology; D007124:Immunoenzyme Techniques; D008297:Male; D000078792:Midkine; D009203:Myocardial Infarction / Q000378:metabolism*; D009206:Myocardium / Q000378:metabolism*; D012333:RNA, Messenger / Q000235:genetics; D051381:Rats; D017208:Rats, Wistar; D013997:Time Factors
D002352:Carrier Proteins; D016207:Cytokines; D006133:Growth Substances; D012333:RNA, Messenger; D000078792:Midkine