A novel EspA-associated surface organelle of enteropathogenic Escherichia coli involved in protein translocation into epithelial cells.
Enteropathogenic Escherichia coli (EPEC), like many bacterial pathogens, employ a type III secretion system to deliver effector proteins across the bacterial cell. In EPEC, four proteins are known to be exported by a type III secretion system_EspA, EspB and EspD required for subversion of host cell signal transduction pathways and a translocated intimin receptor (Tir) protein (formerly Hp90) which is tyrosine-phosphorylated following transfer to the host cell to become a receptor for intimin-mediated intimate attachment and 'attaching and effacing' (A/E) lesion formation. The structural basis for protein translocation has yet to be fully elucidated for any type III secretion system. Here, we describe a novel EspA-containing filamentous organelle that is present on the bacterial surface during the early stage of A/E lesion formation, forms a physical bridge between the bacterium and the infected eukaryotic cell surface and is required for the translocation of EspB into infected epithelial cells.
Knutton
S
S
Institute of Child Health, University of Birmingham, Birmingham B16 8ET, UK. S.KNUTTON@bham.ac.uk
Rosenshine
I
I
Pallen
M J
MJ
Nisan
I
I
Neves
B C
BC
Bain
C
C
Wolff
C
C
Dougan
G
G
Frankel
G
G
D037361:Adenylate Cyclase Toxin; D000818:Animals; D000907:Antibodies, Bacterial / Q000378:metabolism; D001422:Bacterial Adhesion / Q000502:physiology; D001425:Bacterial Outer Membrane Proteins / Q000235:genetics / Q000378:metabolism; D001426:Bacterial Proteins / Q000235:genetics / Q000276:immunology / Q000378:metabolism*; D002462:Cell Membrane / Q000378:metabolism; D004847:Epithelial Cells / Q000378:metabolism / Q000382:microbiology*; D004926:Escherichia coli / Q000378:metabolism*; D029968:Escherichia coli Proteins; D005260:Female; D010861:Fimbriae, Bacterial / Q000378:metabolism / Q000648:ultrastructure; D006367:HeLa Cells; D006801:Humans; D011498:Protein Precursors / Q000235:genetics / Q000378:metabolism; D011817:Rabbits; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000378:metabolism; D014407:Tumor Cells, Cultured
D037361:Adenylate Cyclase Toxin; D000907:Antibodies, Bacterial; D001425:Bacterial Outer Membrane Proteins; D001426:Bacterial Proteins; C087863:EaeB protein, E coli; D029968:Escherichia coli Proteins; C102051:EspA protein, E coli; D011498:Protein Precursors; D011993:Recombinant Fusion Proteins