Absence of mutations in the coding region of the alpha-synuclein gene in pathologically proven Parkinson's disease.
A missense mutation of the alpha-synuclein gene has been associated with parkinsonism in a large Italian kindred. Recently, alpha-synuclein was also identified in Lewy bodies. Using reverse transcribed-polymerase chain reaction (RT-PCR) technique, we sequenced the entire coding region of the alpha-synuclein gene using brain tissue from 24 pathologically proven Parkinson's disease cases. No mutations were found in any of the patients, suggesting that a mutation at the coding region of the alpha-synuclein gene is unlikely to be responsible for nigrostriatal degeneration in typical sporadic Parkinson's disease.
Chan
P
P
Parkinson's Institute, Sunnyvale, CA 94089, USA.
Jiang
X
X
Forno
L S
LS
Di Monte
D A
DA
Tanner
C M
CM
Langston
J W
JW
D000328:Adult; D000368:Aged; D004252:DNA Mutational Analysis; D018076:DNA, Complementary; D005260:Female; D006801:Humans; D008297:Male; D009419:Nerve Tissue Proteins / Q000235:genetics*; D010300:Parkinson Disease / Q000235:genetics*; D017354:Point Mutation; D016133:Polymerase Chain Reaction; D051843:Synucleins; D051844:alpha-Synuclein
D018076:DNA, Complementary; D009419:Nerve Tissue Proteins; C497604:SNCA protein, human; D051843:Synucleins; D051844:alpha-Synuclein