Bone matrix proteins: isolation and characterization of a novel cell-binding keratan sulfate proteoglycan (osteoadherin) from bovine bone.
A small cell-binding proteoglycan for which we propose the name osteoadherin was extracted from bovine bone with guanidine hydrochloride-containing EDTA. It was purified to homogeneity using a combination of ion-exchange chromatography, hydroxyapatite chromatography, and gel filtration. The Mof the proteoglycan was 85, 000 as determined by SDS-PAGE. The protein is rich in aspartic acid, glutamic acid, and leucine. Two internal octapeptides from the proteoglycan contained the sequences Glu-Ile-Asn-Leu-Ser-His-Asn-Lys and Arg-Asp-Leu-Tyr-Phe-Asn-Lys-Ile. These sequences are not previously described, and support the notion that osteoadherin belongs to the family of leucine-rich repeat proteins. A monospecific antiserum was raised in rabbits. An enzyme-linked immunosorbent assay was developed, and showed the osteoadherin content of bone extracts to be 0.4 mg/g of tissue wet weight, whereas none was found in extracts of various other bovine tissues. Metabolic labeling of primary bovine osteoblasts followed by immunoprecipitation showed the cells to synthesize and secrete the proteoglycan. Digesting the immunoprecipitated osteoadherin with N-glycosidase reduced its apparent size to 47 kD, thus showing the presence of several N-linked oligosaccharides. Digestion with keratanase indicated some of the oligosaccharides to be extended to keratan sulfate chains. In immunohistochemical studies of the bovine fetal rib growth plate, osteoadherin was exclusively identified in the primary bone spongiosa. Osteoadherin binds to hydroxyapatite. A potential function of this proteoglycan is to bind cells, since we showed it to be as efficient as fibronectin in promoting osteoblast attachment in vitro. The binding appears to be mediated by the integrin alphavbeta3, since this was the only integrin isolated by osteoadherin affinity chromatography of surface-iodinated osteoblast extracts.
Wendel
M
M
Department of Cell and Molecular Biology, University of Lund, S-221 00 Lund, Sweden. mikael.wendel@medkem.lu.se
Sommarin
Y
Y
Heinegård
D
D
D000595:Amino Acid Sequence; D000818:Animals; D001857:Bone Matrix / Q000737:chemistry* / Q000378:metabolism; D002241:Carbohydrates / Q000032:analysis; D002417:Cattle; D002448:Cell Adhesion; D002478:Cells, Cultured; D011508:Chondroitin Sulfate Proteoglycans / Q000737:chemistry / Q000302:isolation & purification* / Q000378:metabolism; D016023:Integrins / Q000378:metabolism; D007632:Keratan Sulfate / Q000737:chemistry / Q000302:isolation & purification* / Q000378:metabolism; D000071234:Lumican; D010006:Osteoblasts / Q000378:metabolism; D010455:Peptides / Q000737:chemistry; D011817:Rabbits; D014018:Tissue Distribution
D002241:Carbohydrates; D011508:Chondroitin Sulfate Proteoglycans; D016023:Integrins; D000071234:Lumican; D010455:Peptides; D007632:Keratan Sulfate