title: Molecular genetic dissection of mouse unconventional myosin-VA: tail region mutations.
abstract: We used an RT-PCR-based sequencing approach to identify the mutations responsible for 17 viable dilute alleles, a mouse-coat-color locus encoding unconventional myosin-VA. Ten of the mutations mapped to the MyoVA tail and are reported here. These mutations represent the first extensive collection of tail mutations reported for any unconventional mammalian myosin. They identify sequences important for tail function and identify domains potentially involved in cargo binding and/or proper folding of the MyoVA tail. Our results also provide support for the notion that different myosin tail isoforms produced by alternative splicing encode important cell-type-specific functions.
authors.affiliation: ABL-Basic Research Program, NCI-Frederick Cancer Research and Development Center, Maryland 21702, USA.
authors.forename: J D
authors.initials: JD
authors.lastname: Huang
authors.forename: V
authors.initials: V
authors.lastname: Mermall
authors.forename: M C
authors.initials: MC
authors.lastname: Strobel
authors.forename: L B
authors.initials: LB
authors.lastname: Russell
authors.forename: M S
authors.initials: MS
authors.lastname: Mooseker
authors.forename: N G
authors.initials: NG
authors.lastname: Copeland
authors.forename: N A
authors.initials: NA
authors.lastname: Jenkins
mesh_terms: D000818:Animals; D001483:Base Sequence; D004247:DNA; D005260:Female; D015870:Gene Expression; D007381:Intermediate Filament Proteins / Q000096:biosynthesis / Q000235:genetics*; D008297:Male; D051379:Mice; D008809:Mice, Inbred C3H; D008810:Mice, Inbred C57BL; D008969:Molecular Sequence Data; D016296:Mutagenesis; D018995:Myosin Heavy Chains; D024701:Myosin Type V; D012333:RNA, Messenger; D017384:Sequence Deletion
chemical_list: D007381:Intermediate Filament Proteins; C513082:Myo5a protein, mouse; D012333:RNA, Messenger; D004247:DNA; D024701:Myosin Type V; D018995:Myosin Heavy Chains