Contractile responses of proximal and distal trachea segments isolated from rats subjected to immunological stimulation: role of connective tissue mast cells.
1. Anaphylaxis-induced contractions of proximal and distal tracheal segments isolated from 14-day ovalbumin (OA)-sensitized rats were studied. 2. OA-induced contractions in distal segments were significantly greater than those observed in proximal segments. 3. Pretreatment of the rats with compound 48/80 or with sodium cromoglycate (SCG) aerosolization significantly reduced OA-induced contractions of trachea distal segments, whereas the contractions of proximal segments were reduced only by compound 48/80. 4. Mepacrine reduced and indomethacin increased the OA-induced contractions in all tracheal segments. Nor-dihydroguaiaretic acid increased the OA-induced contractions in distal tracheal segments, whereas dazoxiben inhibited the contractions in these same segments; neither of these drugs had any effect on the contractions in proximal tracheal segments. 5. The depletion of connective tissue mast cells and subsequent in vitro treatment with indomethacin increased the OA-induced contractions in both segments. 6. We conclude that the contractions of tracheal muscle from OA-sensitized rats depends on the topographic and anatomical origin of the airway tissue. 7. Mediators released by connective tissue mast cells in proximal and distal segments play a pivotal role in this response and may account for variations in the intensity of contraction seen after the addition of OA.
de Lima
W T
WT
Department of Pharmacology, Institute of Biomedical Sciences, University of São Paulo, Brazil. wtdelima@biomed.icb2.usp.br
da Silva
Z L
ZL
D000707:Anaphylaxis / Q000139:chemically induced* / Q000276:immunology; D000818:Animals; D018927:Anti-Asthmatic Agents / Q000494:pharmacology*; D004205:Cromolyn Sodium / Q000494:pharmacology*; D016861:Cyclooxygenase Inhibitors / Q000494:pharmacology; D007213:Indomethacin / Q000494:pharmacology; D008297:Male; D008407:Mast Cells / Q000187:drug effects* / Q000502:physiology; D009119:Muscle Contraction / Q000187:drug effects*; D010047:Ovalbumin / Q000494:pharmacology; D051381:Rats; D017208:Rats, Wistar; D014132:Trachea / Q000187:drug effects* / Q000502:physiology
D018927:Anti-Asthmatic Agents; D016861:Cyclooxygenase Inhibitors; D010047:Ovalbumin; D004205:Cromolyn Sodium; D007213:Indomethacin