Phosphopeptides derived from in vitro phosphorylated E. coli RNA polymerase bind to DNA and affect DNA transcription.
E. Coli RNA polymerase was phosphorylated with protein kinase CKII and allowed to bind to pBR322. After digestion of the RNA polymerase-pBR322 complex with proteinase K, the phosphopeptides that remained bound to DNA were extracted and analyzed. These phosphopeptides are able to bind again to DNA and to inhibit transcription.
Cardellini
E
E
Dipartimento di Biologia Cellulare e Molecolare, Sezione Fisiologia e Biofisica, Università di Perugia, Italy.
Piccinini
G
G
Gianfranceschi
G L
GL
D047390:Casein Kinase II; D004247:DNA / Q000378:metabolism*; D012321:DNA-Directed RNA Polymerases / Q000378:metabolism*; D015180:Electrophoresis, Gel, Two-Dimensional; D019286:Endopeptidase K / Q000378:metabolism; D004926:Escherichia coli / Q000201:enzymology; D010748:Phosphopeptides / Q000378:metabolism*; D010766:Phosphorylation; D017346:Protein Serine-Threonine Kinases / Q000378:metabolism; D014158:Transcription, Genetic
D010748:Phosphopeptides; D004247:DNA; D047390:Casein Kinase II; D017346:Protein Serine-Threonine Kinases; D012321:DNA-Directed RNA Polymerases; D019286:Endopeptidase K