title: Influenza virus-infected epithelial cells present viral antigens to antigen-specific CD8+ cytotoxic T lymphocytes.
abstract: We have investigated the mechanisms involved in the clearance of viral infection at the epithelium level by analyzing the activity of influenza virus-specific cytotoxic T lymphocytes (CTL) against virus-infected CMT-93 intestinal epithelial cells. Epithelial cells infected with live influenza virus effectively present viral antigens and were lysed by both homotypic and heterotypic influenza virus-specific CD8+ T cells. These results shed new light on the control of viral infection through the elimination of virus-infected epithelial cells by virus-specific CTL and demonstrate that CMT-93 cells furnish an appropriate model for in vitro evaluation of CTL activity against virus-infected epithelial cells.
authors.affiliation: Department of Microbiology and The Immunobiology Vaccine Center, University of Alabama at Birmingham, 35294-2170, USA. nghuan@uab.edu
authors.forename: H H
authors.initials: HH
authors.lastname: Nguyen
authors.forename: P N
authors.initials: PN
authors.lastname: Boyaka
authors.forename: Z
authors.initials: Z
authors.lastname: Moldoveanu
authors.forename: M J
authors.initials: MJ
authors.lastname: Novak
authors.forename: H
authors.initials: H
authors.lastname: Kiyono
authors.forename: J R
authors.initials: JR
authors.lastname: McGhee
authors.forename: J
authors.initials: J
authors.lastname: Mestecky
mesh_terms: D000818:Animals; D000956:Antigens, Viral / Q000276:immunology*; D004847:Epithelial Cells / Q000276:immunology* / Q000821:virology; D006168:Guinea Pigs; D006801:Humans; D009980:Influenza A virus / Q000276:immunology*; D051379:Mice; D008810:Mice, Inbred C57BL; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology*; D014407:Tumor Cells, Cultured
chemical_list: D000956:Antigens, Viral