title: Modulation of DNA polymerases alpha, delta and epsilon by lactate dehydrogenase and 3-phosphoglycerate kinase.
abstract: Literature documents that glycolytic enzymes (among them lactate dehydrogenase and 3-phosphoglycerate kinase) can reside in nuclei of mammalian cells and exert functions in DNA replication, transcription and DNA repair, in addition to their role as catalysts in the cytoplasm. Transfer of glycolytic enzymes to cell nuclei requires modification, for example phosphorylation. We studied the effects of phosphorylated lactate dehydrogenase and 3-phosphoglycerate kinase on (i) UV-induced DNA repair, using permeabilized human fibroblasts, and (ii) in vitro DNA synthesis catalyzed by purified DNA polymerases alpha, delta, and epsilon from proliferating rat liver. (i) Phosphorylated lactate dehydrogenase stimulated UV-induced DNA repair synthesis in normal fibroblasts in a dose-dependent manner; the unphosphorylated enzyme slightly inhibited. In repair-deficient xeroderma pigmentosum fibroblasts reparative synthesis was not enhanced whether lactate dehydrogenase was phosphorylated or not, indicating that reparative DNA synthesis must be possible in order to be stimulated. (ii) Activity of purified DNA polymerases alpha, delta, and epsilon was differentially stimulated or inhibited, according to the phosphorylation status of lactate dehydrogenase. DNA polymerases were also modulated by 3-phosphoglycerate kinase, depending on the primer-templates used which were gapped DNA (mimicking a repair mode of DNA synthesis) or single-stranded M13 DNA (representing the replicative mode of DNA synthesis). Since glycolytic enzymes in cell nuclei retain binding ability for their cofactors, cytoplasmic substrates and inhibitors, a regulatory linkage might exist between the energy state of a cell and its replicative and reparative functions.
authors.affiliation: German Cancer Research Center, Interaction of Carcinogens with Biological Macromolecules Division, Heidelberg, Germany.
authors.forename: O
authors.initials: O
authors.lastname: Popanda
authors.forename: G
authors.initials: G
authors.lastname: Fox
authors.forename: H W
authors.initials: HW
authors.lastname: Thielmann
mesh_terms: D000818:Animals; D002467:Cell Nucleus / Q000201:enzymology; D004247:DNA / Q000096:biosynthesis; D004256:DNA Polymerase I / Q000378:metabolism*; D004257:DNA Polymerase II / Q000378:metabolism*; D004258:DNA Polymerase III / Q000378:metabolism*; D004260:DNA Repair / Q000187:drug effects / Q000528:radiation effects; D004277:DNA, Single-Stranded / Q000378:metabolism; D005347:Fibroblasts / Q000528:radiation effects; D006801:Humans; D007150:Immunohistochemistry; D007770:L-Lactate Dehydrogenase / Q000378:metabolism* / Q000494:pharmacology; D010539:Permeability / Q000528:radiation effects; D010735:Phosphoglycerate Kinase / Q000378:metabolism*; D010766:Phosphorylation; D051381:Rats; D014466:Ultraviolet Rays; D014983:Xeroderma Pigmentosum / Q000473:pathology
chemical_list: D004277:DNA, Single-Stranded; D004247:DNA; D007770:L-Lactate Dehydrogenase; D010735:Phosphoglycerate Kinase; D004256:DNA Polymerase I; D004257:DNA Polymerase II; D004258:DNA Polymerase III