Immunosuppressive components from an Ascomycete, Diplogelasinospora grovesii.
Two known fungal metabolites, macrophin and colletodiol, and a new stereoisomer of colletodiol named 10-epi-colletodiol, were isolated as immunosuppressive principles from an Ascomycete, Diplogelasinospora grovesii. The IC50 values of the major active component among them, macrophin, were calculated to be 0.4 and 0.3 microgram/ml against concanavalin A- and lipopolysaccharide-induced proliferations of mouse spleen lymphocytes, respectively. A new natural product, 4,8-dimethyl-1,5-dioxacyclooctane-2,6-dione, and a known fungal metabolite, isosclerone, which showed no immunosuppressive activity, were also isolated from this fungus.
Fujimoto
H
H
Faculty of Pharmaceutical Sciences, Chiba University, Japan.
Nagano
J
J
Yamaguchi
K
K
Yamazaki
M
M
D000818:Animals; D001203:Ascomycota / Q000737:chemistry*; D002455:Cell Division / Q000187:drug effects; D002478:Cells, Cultured / Q000187:drug effects; D018360:Crystallography, X-Ray; D007166:Immunosuppressive Agents / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D007783:Lactones / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D009682:Magnetic Resonance Spectroscopy; D051379:Mice; D013154:Spleen / Q000166:cytology / Q000187:drug effects; D013237:Stereoisomerism
C111698:4,8-dimethyl-1,5-dioxacyclooctane-2,6-dione; D007166:Immunosuppressive Agents; D007783:Lactones; C111697:colletodiol