[Restriction endonuclease Sse9I from Sporosarcina sp. strain 9D recognizes the 5'-AATT-3' DNA sequence].
A new restriction endonuclease Sse9I was isolated from the bacterial strain Sporosarcina sp. 9D. The enzyme belongs to Type II restrictases and recognizes the tetranucleotide sequence 5'-AATT-3'. The enzyme cleaves DNA before the first adenine residue, so it is a true isoschizomer of Tsp509I restrictase. However, unlike the prototype, Sse9I digests DNA at 55 degrees C and loses its activity after 20 min storage at 65 degrees C.
Gonchar
D A
DA
SibEnzyme, Novosibirsk, Russia.
Dedkov
V S
VS
Verkhozina
V A
VA
Kusner
Iu S
IuS
Shevchenko
A V
AV
Degtiarev
S Kh
SKh
D004247:DNA / Q000378:metabolism*; D016936:Gram-Positive Endospore-Forming Bacteria / Q000201:enzymology*; D006358:Hot Temperature; D006868:Hydrolysis; D015265:Site-Specific DNA-Methyltransferase (Adenine-Specific) / Q000037:antagonists & inhibitors / Q000378:metabolism*; D013379:Substrate Specificity
D004247:DNA; C100875:DNA methyltransferase SSe9I; D015265:Site-Specific DNA-Methyltransferase (Adenine-Specific)