Pancreatic IL-10 induces diabetes in NOD.B6 Idd3 Idd10 mice.
Transgenic NOD backcross mice expressing pancreatic interleukin 10 (IL-10) were crossed and backcrossed to NOD.B6 Idd3 Idd10 mice, which have diabetes-resistance alleles at Idd3 and Idd10 on chromosome 3 and have a very low frequency diabetes and insulitis. Insulitis and diabetes developed in almost all IL-10 transgenic backcross 1 (BC1) mice of the H2g(7/g7) haplotype regardless of the allelic status at Idd3 and Idd10. Furthermore, diabetes occurred in 23% of IL-10 transgenic H2g(7/d) BC1 mice. These results indicate that pancreatic IL-10 is able to overcome the diabetes protection afforded by C57BL/6 (B6)-derived alleles at Idd3 and Idd10 as well as the absence of NOD MHC homozygosity, if other non-MHC NOD-derived Idd alleles are provided.
Lee
M S
MS
Department of Neuropharmacology, The Scripps Research Institute, La Jolla, CA 92037, USA.
Wicker
L S
LS
Peterson
L B
LB
Sarvetnick
N
N
D000483:Alleles; D000818:Animals; D003920:Diabetes Mellitus / Q000276:immunology; D005260:Female; D007178:Inbreeding; D016753:Interleukin-10 / Q000235:genetics / Q000276:immunology*; D008297:Male; D051379:Mice; D016688:Mice, Inbred NOD / Q000276:immunology*; D008822:Mice, Transgenic; D010179:Pancreas / Q000276:immunology
D016753:Interleukin-10