[Drug delivery systems in gene therapy].
The use of nonviral vectors is an attractive in vivo gene delivery strategy that is simpler and lacks some risks inherent in viral systems. Liposomes and receptor-mediated polycation systems are promising carriers for delivery and expression of plasmid DNA encoding genes into the target cells. Many barriers need to be overcome for successful in vivo DNA delivery using these carrier systems. Various factors such as the extent of DNA condensation, particle size of the DNA complex, route of administration, stability against nucleases, target sites, in vivo disposition, binding to cell surface receptor and internalization, intracellular trafficking affect the in vivo gene delivery and expression. This chapter will focus on the current status and perspectives of the plasmid DNA delivery systems for in vivo gene therapy.
Takakura
Y
Y
Graduate School of Pharmaceutical Sciences, Kyoto University.
D004247:DNA / Q000008:administration & dosage*; D004337:Drug Carriers; D016503:Drug Delivery Systems; D015316:Genetic Therapy; D006801:Humans; D008081:Liposomes; D016376:Oligonucleotides, Antisense / Q000008:administration & dosage; D010957:Plasmids; D011073:Polyamines; D000071228:Polyelectrolytes
D004247:DNA; D004337:Drug Carriers; D008081:Liposomes; D016376:Oligonucleotides, Antisense; D011073:Polyamines; D000071228:Polyelectrolytes