Triosephosphate metabolism by mature boar spermatozoa.
Boar sperm rapidly interconverted dihydroxyacetone phosphate and glyceraldehyde 3-phosphate, produced fructose-1,6-bisphosphate, approximately equilibrium concentrations of fructose 6-phosphate and glucose 6-phosphate but not glycerol or glycerol 3-phosphate. In the presence of 3-chloro-1-hydroxypropanone, an inhibitor of stage 2 of the glycolytic pathway, the triosephosphates were metabolized faster, produced less fructose-1,6-bisphosphate, fructose 6-phosphate and glucose 6-phosphate, but not glycerol or glycerol 3-phosphate. This suggests that these cells may have the capacity to convert glycolytic intermediates into a storage metabolite to conserve carbon atoms for the eventual synthesis of lactate.
Jones
A R
AR
Department of Biochemistry, University of Sydney, NSW, Australia. A.jones@biochem.usyd.edu.au
D000096:Acetone / Q000031:analogs & derivatives; D000818:Animals; D004099:Dihydroxyacetone Phosphate / Q000378:metabolism; D004791:Enzyme Inhibitors / Q000494:pharmacology; D005635:Fructosediphosphates / Q000378:metabolism; D005636:Fructosephosphates / Q000378:metabolism; D019298:Glucose-6-Phosphate / Q000378:metabolism; D005986:Glyceraldehyde 3-Phosphate / Q000378:metabolism; D005987:Glyceraldehyde-3-Phosphate Dehydrogenases / Q000037:antagonists & inhibitors; D007700:Kinetics; D019344:Lactic Acid / Q000378:metabolism; D008297:Male; D020005:Propanols / Q000494:pharmacology; D013094:Spermatozoa / Q000378:metabolism*; D013403:Sugar Phosphates / Q000378:metabolism*; D013552:Swine; D014305:Triose-Phosphate Isomerase / Q000037:antagonists & inhibitors
D004791:Enzyme Inhibitors; D005635:Fructosediphosphates; D005636:Fructosephosphates; D020005:Propanols; D013403:Sugar Phosphates; D000096:Acetone; D005986:Glyceraldehyde 3-Phosphate; C110397:3-chloro-1-propanol; D019344:Lactic Acid; D019298:Glucose-6-Phosphate; D004099:Dihydroxyacetone Phosphate; C027618:fructose-6-phosphate; D005987:Glyceraldehyde-3-Phosphate Dehydrogenases; D014305:Triose-Phosphate Isomerase; C029063:fructose-1,6-diphosphate; C027160:1-hydroxy-3-chloroacetone