IL-10 production in common variable immunodeficiency.
Common variable immunodeficiency, (CVI) is a heterogeneous primary immunodeficiency disease in which there are T and B cell defects. Since IL-10 in conjunction with anti-CD40 promotes secretion of IgG, IgA, and IgM by CVI B cells, these studies were performed to investigate IL-10 production in CVI. Mitogen or anti-CD3 stimulated CVI peripheral blood mononuclear cells, or isolated T cells produced an insignificant amount of IL-10 over background levels. CVI monocyte IL-10 production was substantial and greater than that of normal controls. Anti-IL-10-neutralizing antibody strongly enhanced CVI T cell proliferative responses to PHA, but only to an insignificant extent, soluble antigens. IL-2 plus anti-IL-10 enhanced CVI proliferative responses to antigens significantly more over baseline than for cells of similarly tested normal controls. These data suggest that CVI T cell secretion of IL-10 is deficient, but that monocyte-derived IL-10, plus a relative lack of IL-2 production, could contribute to the defects of cell proliferation in this disorder.
Zhou
Z
Z
Division of Clinical Immunology, Mt. Sinai Medical Center, New York, New York 10029, USA.
Huang
R
R
Danon
M
M
Mayer
L
L
Cunningham-Rundles
C
C
D000293:Adolescent; D000328:Adult; D000368:Aged; D000941:Antigens / Q000008:administration & dosage; D002648:Child; D017074:Common Variable Immunodeficiency / Q000276:immunology* / Q000628:therapy; D005260:Female; D006801:Humans; D007108:Immune Tolerance; D016756:Immunoglobulins, Intravenous / Q000627:therapeutic use; D066298:In Vitro Techniques; D016753:Interleukin-10 / Q000037:antagonists & inhibitors / Q000096:biosynthesis* / Q000494:pharmacology; D007376:Interleukin-2 / Q000494:pharmacology; D008213:Lymphocyte Activation; D008264:Macrophages / Q000276:immunology; D008297:Male; D008875:Middle Aged; D009000:Monocytes / Q000276:immunology; D009500:Neutralization Tests; D010835:Phytohemagglutinins / Q000008:administration & dosage; D013601:T-Lymphocytes / Q000276:immunology
D000941:Antigens; D016756:Immunoglobulins, Intravenous; D007376:Interleukin-2; D010835:Phytohemagglutinins; D016753:Interleukin-10