A methylation imprint mark in the mouse imprinted gene Grf1/Cdc25Mm locus shares a common feature with the U2afbp-rs gene: an association with a short tandem repeat and a hypermethylated region.
We identified a sperm-specific methylation imprint mark (Site II) associated with a short tandem repeat sequence and a site/region methylated in both gametes (Site I) in the Grf1 locus on mouse chromosome 9, which shared a common feature with the U2afbp-rs gene. Sites or regions of gamete-specific methylation in imprinted genes are strong candidates for carrying information regarding the parental origin of alleles. The gamete-specific methylation pattern of Sites I and II was conserved after fertilization, but attained the somatic cell pattern by the blastocyst stage. In primordial germ cells, Site I was methylated, but Site II was unmethylated in both male and female embryos, suggesting that the sperm-specific methylation imprint mark in Site II was established during spermatogenesis. These common features in methylation imprint regions may be a clue to identifying regions carrying primary information for the imprinting regulation.
Shibata
H
H
Genome Science Laboratory, RIKEN Tsukuba Life Science Center, Ibaraki, Japan.
Yoda
Y
Y
Kato
R
R
Ueda
T
T
Kamiya
M
M
Hiraiwa
N
N
Yoshiki
A
A
Plass
C
C
Pearsall
R S
RS
Held
W A
WA
Muramatsu
M
M
Sasaki
H
H
Kusakabe
M
M
Hayashizaki
Y
Y
D000818:Animals; D001665:Binding Sites; D018797:Cell Cycle Proteins / Q000235:genetics*; D002874:Chromosome Mapping; D019175:DNA Methylation; D047108:Embryonic Development; D005260:Female; D018392:Genomic Imprinting; D020662:Guanine Nucleotide Exchange Factors; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D008969:Molecular Sequence Data; D009419:Nerve Tissue Proteins; D009687:Nuclear Proteins; D010749:Phosphoprotein Phosphatases / Q000235:genetics*; D063146:Polycomb-Group Proteins; D011247:Pregnancy; D011506:Proteins / Q000235:genetics*; D012091:Repetitive Sequences, Nucleic Acid; D012097:Repressor Proteins / Q000235:genetics*; D012261:Ribonucleoproteins / Q000235:genetics*; D000072668:Splicing Factor U2AF; D020663:ras Guanine Nucleotide Exchange Factors; D020666:ras-GRF1
D018797:Cell Cycle Proteins; D020662:Guanine Nucleotide Exchange Factors; D009419:Nerve Tissue Proteins; D009687:Nuclear Proteins; D063146:Polycomb-Group Proteins; D011506:Proteins; D012097:Repressor Proteins; D012261:Ribonucleoproteins; C112628:SCML1 protein, human; D000072668:Splicing Factor U2AF; C000607495:Zrsr1 protein, mouse; D020663:ras Guanine Nucleotide Exchange Factors; D020666:ras-GRF1; D010749:Phosphoprotein Phosphatases