Molecular cloning, structural characterization, and chromosomal mapping of the human LECT2 gene.
We originally isolated LECT2 (leukocyte cell-derived chemotaxin 2) as a 16-kDa secreted protein having a human neutrophil chemotactic activity, then cloned human and bovine LECT2 cDNAs and demonstrated the liver-specific expression of the protein. LECT2 is thought to be a multifunctional protein, because it was recently found to be identical to chondromodulin-II a growth stimulator of chondrocyte cells. We report here the cloning and the structural analysis of the human LECT2 gene. The gene spans approximately 8 kb and consists of four exons and three introns. Primer extension analysis revealed that several transcription initiation sites occur within 70-230 nucleotides upstream of the translation initiation codon. Several transcriptional control sequences relevant to the liver-specific expression have been identified at the 5' untranslated region of the human LECT2 gene. The human LECT2 gene was mapped to chromosome 5q31.1-q32 by fluorescence in situ hybridization. This region contains a cluster of cytokine genes including IL-4, IL-5, and IL-9.
Yamagoe
S
S
Department of Bioactive Molecules, National Institute of Infectious Diseases, Tokyo, Japan.
Kameoka
Y
Y
Hashimoto
K
K
Mizuno
S
S
Suzuki
K
K
D001483:Base Sequence; D001665:Binding Sites; D002460:Cell Line; D002630:Chemotactic Factors / Q000737:chemistry / Q000235:genetics*; D002634:Chemotaxis, Leukocyte; D002874:Chromosome Mapping; D002895:Chromosomes, Human, Pair 5; D003001:Cloning, Molecular; D018922:HL-60 Cells; D006801:Humans; D036341:Intercellular Signaling Peptides and Proteins; D008969:Molecular Sequence Data; D011110:Polymorphism, Genetic; D011506:Proteins / Q000737:chemistry / Q000235:genetics*; D014157:Transcription Factors / Q000378:metabolism; D014158:Transcription, Genetic
D002630:Chemotactic Factors; D036341:Intercellular Signaling Peptides and Proteins; C491214:LECT2 protein, Bos taurus; C103468:LECT2 protein, human; D011506:Proteins; D014157:Transcription Factors