Involvement of reactive oxygen species in phospholipase A2 activation: inhibition of protein tyrosine phosphatases and activation of protein kinases.
Activators of PKC in combination with vanadate induce massive formation of reactive oxygen species. The formation of ROS leads to suppression of protein tyrosine phosphatase activity and consequently to enhanced protein tyrosine phosphorylation. This culminates in the activation of the MAP-K cascade and of PLA2 (Scheme 1).
Goldman
R
R
Department of Membrane Research and Biophysics, Weizmann Institute of Science, Rehovot, Israel.
Ferber
E
E
Zor
U
U
D000818:Animals; D001854:Bone Marrow Cells / Q000166:cytology; D017871:Calcium-Calmodulin-Dependent Protein Kinases / Q000378:metabolism*; D002454:Cell Differentiation; D002462:Cell Membrane / Q000201:enzymology; D004789:Enzyme Activation; D006861:Hydrogen Peroxide / Q000494:pharmacology; D007700:Kinetics; D008264:Macrophages / Q000201:enzymology / Q000502:physiology*; D051379:Mice; D010741:Phospholipases A / Q000378:metabolism*; D054467:Phospholipases A2; D011494:Protein Kinases / Q000378:metabolism*; D017027:Protein Tyrosine Phosphatases / Q000037:antagonists & inhibitors*; D017382:Reactive Oxygen Species / Q000502:physiology*; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology; D014638:Vanadates / Q000494:pharmacology; D015054:Zymosan / Q000494:pharmacology
D017382:Reactive Oxygen Species; D014638:Vanadates; D015054:Zymosan; D006861:Hydrogen Peroxide; D011494:Protein Kinases; D017871:Calcium-Calmodulin-Dependent Protein Kinases; D010741:Phospholipases A; D054467:Phospholipases A2; D017027:Protein Tyrosine Phosphatases; D013755:Tetradecanoylphorbol Acetate