title: Cryopreserved cadaveric allografts for treatment of unexcised partial thickness flame burns: clinical experience with 12 patients.
abstract: Partial thickness burns (PTB) usually heal within 3 weeks. Prevention of infection and desiccation of the wounds are crucial for optimal healing. Early tangential excision of the burn eschar and allografting prevent deepening of the burns, and are therefore advocated for treatment with the best functional and aesthetic results. For superficial partial thickness burns (SPTB) conservative use of topical antimicrobial agents with frequent dressing changes are implemented. We compared the conservative treatment for PTBs and SPTBs to grafting cryopreserved cadaveric allografts with no prior excision. Twelve patients with flame PTB areas were allografted after mechanical debridement without excision of the burn wounds. The allografts were cadaveric skin cryopreserved by programmed freezing and stored at -180 degrees C for 30-48 months. Matching burns for depth and area were treated with silver sulfadiazine (SSD) one to two times daily until healing or debridement and grafting were required. It was found that 80 per cent of the cryopreserved allografts adhered well and 76 per cent of the treated areas healed within 21 days, whereas only 40 per cent of the SSD-treated burns healed within 21 days. Partial thickness burns can be treated successfully with viable human allografts (cryopreserved cadaveric skin) with no prior surgical excision. The burn wounds heal well within 3 weeks. For deep partial thickness burns (DPTB) treatment with allografts has no advantage if they have not been previously excised.
authors.affiliation: Burn Unit, Hadassah University Hospital, Jerusalem, Israel.
authors.forename: A
authors.initials: A
authors.lastname: Eldad
authors.forename: A
authors.initials: A
authors.lastname: Din
authors.forename: A
authors.initials: A
authors.lastname: Weinberg
authors.forename: A
authors.initials: A
authors.lastname: Neuman
authors.forename: H
authors.initials: H
authors.lastname: Lipton
authors.forename: H
authors.initials: H
authors.lastname: Ben-Bassat
authors.forename: M
authors.initials: M
authors.lastname: Chaouat
authors.forename: M R
authors.initials: MR
authors.lastname: Wexler
mesh_terms: D000293:Adolescent; D000328:Adult; D002056:Burns / Q000503:physiopathology / Q000601:surgery*; D002102:Cadaver; D002648:Child; D015925:Cryopreservation / Q000379:methods*; D005260:Female; D006085:Graft Survival; D006801:Humans; D015601:Injury Severity Score; D008297:Male; D008875:Middle Aged; D011379:Prognosis; D012867:Skin / Q000503:physiopathology; D016038:Skin Transplantation / Q000379:methods*; D014182:Transplantation, Autologous; D014184:Transplantation, Homologous / Q000379:methods*; D014945:Wound Healing