title: Validation of an LC-MS assay for the quantification of the enantiomers of Org 4428 in human plasma.
abstract: A sensitive and selective liquid chromatographic mass spectrometric assay has been validated for the quantification of Org 4428 enantiomers in human plasma. The assay employs n-hexane extraction from alkalinized plasma, separation on a narrow-bore enantioselective normal phase Chiralpak AD column and APCI MS-MS detection. The lower limit of quantification is 0.5 ng ml-1 plasma for the individual enantiomers.
authors.affiliation: Scientific Development Group, N.V. Organon, Department of Drug Metabolism and Kinetics, Oss, Netherlands.
authors.forename: J E
authors.initials: JE
authors.lastname: Paanakker
authors.forename: J
authors.initials: J
authors.lastname: de Jong
authors.forename: J M
authors.initials: JM
authors.lastname: Thio
authors.forename: H J
authors.initials: HJ
authors.lastname: van Hal
mesh_terms: D018759:Adrenergic Uptake Inhibitors / Q000097:blood* / Q000737:chemistry; D002138:Calibration; D002853:Chromatography, Liquid; D005615:Freezing; D006801:Humans; D007202:Indicators and Reagents; D013058:Mass Spectrometry; D011725:Pyridines / Q000097:blood* / Q000737:chemistry; D015203:Reproducibility of Results; D013237:Stereoisomerism
chemical_list: D018759:Adrenergic Uptake Inhibitors; D007202:Indicators and Reagents; C105480:ORG 4428; D011725:Pyridines