Sulfuryl transfer: the catalytic mechanism of human estrogen sulfotransferase.
Estrogen sulfotransferase (EST) catalyzes the transfer of the sulfuryl group from 3'-phosphoadenosine 5'-phosphosulfate (PAPS) to 17beta-estradiol (E2). The sulfation of E2 prevents it from binding to, and thereby activating, the estrogen receptor. The regulation of EST appears to be causally linked to tumorigenesis in the breast and endometrium. In this study, recombinant human EST is characterized, and the catalytic mechanism of the transfer reaction is investigated in ligand binding and initial rate experiments. The native enzyme is a dimer of 35-kDa subunits. The apparent equilibrium constant for transfer to E2 is (4.5 +/- 0.2) x 10(3) at pH 6.3 and T = 25 +/- 2 degrees C. Initial rate studies provide the kinetic constants for the reaction and suggest a sequential mechanism. E2 is a partial substrate inhibitor (Ki = 80 +/- 5 nM). The binding of two E2 per EST subunit suggests that the partial inhibition occurs through binding at an allosteric site. In addition to providing the dissociation constants for the ligand-enzyme complexes, binding studies demonstrate that each substrate binds independently to the enzyme and that both the E.PAP.E2S and E.PAP.E2 dead-end complexes form. These results strongly suggest a Random Bi Bi mechanism with two dead-end complexes.
Zhang
H
H
Department of Biochemistry, Albert Einstein College of Medicine, Bronx, New York 10461, USA.
Varlamova
O
O
Vargas
F M
FM
Falany
C N
CN
Leyh
T S
TS
Varmalova
O
O
D002384:Catalysis; D002460:Cell Line; D002850:Chromatography, Gel; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D008970:Molecular Weight; D011485:Protein Binding; D013447:Sulfites / Q000378:metabolism*; D015238:Sulfotransferases / Q000737:chemistry / Q000378:metabolism*
D013447:Sulfites; D015238:Sulfotransferases; C020122:estrone sulfotransferase