Effect of glucocorticoid hormone on nuclear matrix in cervical cancer cells in vitro.
Human papillomavirus (HPV) is one of the crucial factors in cervical carcinogenesis. High risk HPV16 prototype has been demonstrated in association with the nuclear matrix in a cervical carcinoma cell line(1,2). Nuclear matrix (NM) has been established as playing an important role in various nuclear activities as well as carcinogenic processes. Dexamethasone (DEX) (glucocorticoid hormone) inhibited the growth of CC2/CUHK2 cervical carcinoma cells with concurrent induction of epithelial cell differentiation. 2D- PAGE (IEF and NEPHGE) revealed alternations in NM protein composition. Further demonstration of changes in NM was evidenced by NuMA (a novel NM protein) labelling. The HPV16 E7 oncoprotein was shown to be reduced in total cellular protein as well as in NM protein fractions in response to DEX treatment, and this suppressed expression was confirmed by RT-PCR. Thus, it is suggested that dexamethasone can down-regulate the growth of cervical cancer cells and its induced changes in NM may be a cause of this suppression.
Yam
H F
HF
Department of Anatomy, Chinese University of Hong Kong, Shatin, PR China.
Wang
Z H
ZH
Or
P C
PC
Wang
S W
SW
Li
J
J
Chew
E C
EC
D002277:Carcinoma / Q000648:ultrastructure*; D002455:Cell Division / Q000187:drug effects; D003907:Dexamethasone / Q000494:pharmacology*; D015180:Electrophoresis, Gel, Two-Dimensional; D005260:Female; D015972:Gene Expression Regulation, Neoplastic / Q000187:drug effects; D015967:Gene Expression Regulation, Viral / Q000187:drug effects; D005938:Glucocorticoids / Q000494:pharmacology*; D006801:Humans; D007150:Immunohistochemistry; D007633:Keratins / Q000378:metabolism; D019394:Ki-67 Antigen / Q000378:metabolism; D015530:Nuclear Matrix / Q000187:drug effects*; D009856:Oncogene Proteins, Viral / Q000235:genetics; D050725:Papillomavirus E7 Proteins; D012334:RNA, Neoplasm / Q000378:metabolism; D014407:Tumor Cells, Cultured; D002583:Uterine Cervical Neoplasms / Q000648:ultrastructure*
D005938:Glucocorticoids; D019394:Ki-67 Antigen; D009856:Oncogene Proteins, Viral; D050725:Papillomavirus E7 Proteins; D012334:RNA, Neoplasm; C059731:oncogene protein E7, Human papillomavirus type 16; D007633:Keratins; D003907:Dexamethasone