[Interaction of short oligonucleotides derivatives with nucleic acids. VI. Discrimination of mismatch-containing complexes upon ligation of a short oligonucleotide tandem on DNA template].
The high ligation specificity of a tetranucleotide with a pair of flanking octanucleotides on DNA template by the action of T4 phage DNA ligase is shown. In a tetranucleotide-DNA template complex containing a mismatch, almost no ligation products are formed. The ligation of a tandem octanucleotide-tetranucleotide-octanucleotide makes it possible to identify accurately any single nucleotide substitution in a tetranucleotide binding site.
Pyshnyĭ
D V
DV
Novosibirsk Institute of Bioorganic Chemistry, Siberian Division, Russian Academy of Sciences, Novosibirsk, Russia.
Krivenko
A A
AA
Lokhov
S G
SG
Ivanova
E M
EM
Dymshits
G M
GM
Zarytova
V F
VF
D001345:Autoradiography; D001665:Binding Sites; D004247:DNA / Q000737:chemistry*; D011088:DNA Ligases / Q000737:chemistry*; D004591:Electrophoresis, Polyacrylamide Gel; D009841:Oligonucleotides / Q000737:chemistry* / Q000235:genetics; D017354:Point Mutation; D012091:Repetitive Sequences, Nucleic Acid; D013696:Temperature; D013698:Templates, Genetic
D009841:Oligonucleotides; D004247:DNA; D011088:DNA Ligases