title: [Use of western blot methods for serodiagnosis of hydatidosis in Poland].
abstract: 46 sera and 1 cerebrospinal fluid samples from 47 patients suspected of hydatidosis were examined by Western blot method, and the results were compared with those of the indirect haemagglutination, immunoelectrophoresis and an enzyme-linked immunosorbent assay (ELISA). The examinations confirmed high sensitivity and specificity of the reactions with 8 kDa antigenic fraction in the Western blot. The above data supported the concept, that serological diagnosis of hydatidosis should be carried out using a high sensitivity screening test, such as ELISA, then the Western blot method to verify positive results.
authors.affiliation: Zakład Parazytologii Lekarskiej, PZH, Warszawa.
authors.forename: E
authors.initials: E
authors.lastname: Bitkowska
authors.forename: E
authors.initials: E
authors.lastname: Gołab
authors.forename: W
authors.initials: W
authors.lastname: Płonka
authors.forename: T H
authors.initials: TH
authors.lastname: Dzbeński
mesh_terms: D015153:Blotting, Western / Q000379:methods*; D002555:Cerebrospinal Fluid / Q000382:microbiology; D004443:Echinococcosis / Q000175:diagnosis*; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D008403:Mass Screening / Q000379:methods*; D011044:Poland; D012680:Sensitivity and Specificity; D012698:Serologic Tests / Q000379:methods*