Ivermectin quantitation with an abamectin internal standard.
The potent antiparasitic ivermectin is detected at low concentrations (ng/g) in liver and muscle tissue by liquid chromatography after conversion to a fluorescent derivative with 1-methylimidazole and trifluoroacetic anhydride. This acetylation reaction can be compromised by residual water that leads to decreased yields. Yields of derivatives of ivermectin and abamectin, a related avermectin, are identical under all circumstances tested. Use of abamectin as an internal standard eliminates derivative yield as a source of analytical variation.
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U.S. Department of Agriculture, Food Safety and Inspection Service, Eastern Laboratory, Richard B. Russell Center, Athens, GA 30604, USA.
D000871:Anthelmintics / Q000032:analysis*; D002853:Chromatography, Liquid; D007202:Indicators and Reagents; D007559:Ivermectin / Q000031:analogs & derivatives / Q000032:analysis*; D008099:Liver / Q000737:chemistry; D009132:Muscles / Q000737:chemistry; D012015:Reference Standards; D013050:Spectrometry, Fluorescence
D000871:Anthelmintics; D007202:Indicators and Reagents; C048324:abamectin; D007559:Ivermectin