Transgenic mouse expressing a full-length hepatitis C virus cDNA.
Hepatitis C virus (HCV), a major causative agent of post transfusion non-A, non-B hepatitis (NANBH), can only infect humans and chimpanzees. We produced nine transgenic mouse lines carrying a full-length HCV cDNA with the human serum amyloid P component (hSAP) promoter that can direct liver-specific expression. In one of these lines HCV mRNA and HCV core protein were detected in the liver of the transgenic mouse, although the levels of expression were very low. In addition, HCV-related antibody was detected in the serum.
Matsuda
J
J
The Chemo-Sero-Therapeutic Research Institute, Kikuchi Research Center, Pathology Department, Kumamoto.
Suzuki
M
M
Nozaki
C
C
Shinya
N
N
Tashiro
K
K
Mizuno
K
K
Uchinuno
Y
Y
Yamamura
K
K
D000410:Alanine Transaminase / Q000097:blood; D000818:Animals; D001219:Aspartate Aminotransferases / Q000097:blood; D004279:DNA, Viral / Q000235:genetics* / Q000378:metabolism; D016174:Hepacivirus / Q000235:genetics*; D018937:Hepatitis C Antibodies / Q000096:biosynthesis / Q000097:blood; D006801:Humans; D007150:Immunohistochemistry; D008099:Liver / Q000378:metabolism / Q000473:pathology; D051379:Mice; D008807:Mice, Inbred BALB C; D008822:Mice, Transgenic; D016133:Polymerase Chain Reaction; D011401:Promoter Regions, Genetic; D012367:RNA, Viral / Q000235:genetics / Q000378:metabolism; D012680:Sensitivity and Specificity; D000683:Serum Amyloid P-Component / Q000378:metabolism; D014158:Transcription, Genetic
D004279:DNA, Viral; D018937:Hepatitis C Antibodies; D012367:RNA, Viral; D000683:Serum Amyloid P-Component; D001219:Aspartate Aminotransferases; D000410:Alanine Transaminase