Interaction of estradiol, progesterone and corticosterone on uterine connective tissue degrading enzymes.
The impact of ovarian hormones and corticosterone acetate on uterine connective tissue degrading enzymes were studied in mature albino rats. Ovariectomy resulted in a significant increase in the activities of alpha- and beta-galactosidases and glucosidases in the uterus. Administration of estradiol to ovariectomized rats brought back the activities of alpha-galactosidase and alpha-glucosidase to normalcy. While beta-galactosidase and beta-glucosidase were significantly decreased. Administration of progesterone to ovariectomized rats resulted in the increase of alpha- and beta-galactosidases and glucosidases. Administration of corticosterone to ovariectomized rats produced a further increase in alpha- and beta-galactosidases and glucosidases in the uterus. Adrenalectomy in ovary intact rats produced a decrease in alpha-galactosidase however, beta-glucosidase was significantly increased. Administration of corticosterone to ovary intact rats significantly increased the activities of alpha- and beta-galactosidases, while alpha- and beta-glucosidases were found to be decreased. Ovariectomy resulted in a significant increase in the activities of cathepsin-D and cathepsin-E. Administration of estradiol to ovariectomized rats brought back the activity of cathepsin-D to normalcy, whereas cathepsin-E was significantly increased. Administration of progesterone as well as estradiol to ovariectomized rats significantly increased the levels of cathepsin-E, however, cathepsin-D was brought back to normalcy. Administration of corticosterone to ovariectomized rats as well as ovariectomy + adrenalectomy significantly increased the activity of cathepsin-D and cathepsin-E. Adrenalectomy significantly decreased the activity of cathepsin-D, while administration of corticosterone increased the cathepsin-D and cathepsin-E in the uterus. Therefore, these results suggest that estradiol is a potent ovarian steroid protecting the extra cellular matrix components. The effect of progesterone appears to modulate and act hand in hand with estradiol. Corticosterone appears to have an opposite effect to that of estradiol.
Gladson
M
M
Department of Endocrinology, Dr. ALM PG Institute of Basic Medical Sciences, University of Madras, Taramani, India.
Srinivasan
N
N
Malini
T
T
Arunakaran
J
J
Aruldhas
M M
MM
Govindarajulu
P
P
D000315:Adrenalectomy; D000818:Animals; D002403:Cathepsins / Q000145:classification / Q000187:drug effects / Q000378:metabolism*; D015331:Cohort Studies; D003238:Connective Tissue / Q000187:drug effects / Q000201:enzymology / Q000378:metabolism; D003345:Corticosterone / Q000494:pharmacology*; D004958:Estradiol / Q000494:pharmacology*; D005109:Extracellular Matrix / Q000187:drug effects / Q000201:enzymology / Q000378:metabolism; D005260:Female; D005696:Galactosidases / Q000145:classification / Q000187:drug effects / Q000378:metabolism*; D005959:Glucosidases / Q000145:classification / Q000187:drug effects / Q000378:metabolism*; D010052:Ovariectomy; D011374:Progesterone / Q000494:pharmacology*; D051381:Rats; D017208:Rats, Wistar; D014599:Uterus / Q000187:drug effects / Q000201:enzymology*
D011374:Progesterone; D004958:Estradiol; D005696:Galactosidases; D005959:Glucosidases; D002403:Cathepsins; D003345:Corticosterone