Physical linkage of the B29/Ig-beta (CD79B) gene to the skeletal muscle, sodium-channel, and growth hormone genes in rat and human.
In the region between the polyadenylation site of the rat skeletal muscle (SkM) Na-channel gene and the 5' end of the growth hormone (GH) gene, a gene coding for B-cell-specific membrane protein B29/Ig-beta was found and noted to have the same orientation as the Na-channel and GH genes. Rat B29/Ig-beta gene was 3.1 kb in length with six exons and was separated by 3.3 and 9.3 kb from Na-channel and GH genes, respectively. Rat B29/Ig-beta protein comprised 228 amino acids, and its amino acid sequence was 85 and 69% identical with the mouse and human counterparts, respectively. With the long-area PCR method, genomic DNA connecting human SkM Na-channel (SCN4A) and B29/Ig-beta (CD79B) genes and CD79B and GH (GH1) genes was amplified, and the physical linkage of SCN4A/CD79B/ GH1 genes in the human genome was established. The human CD79B gene was separated by 6.3 and 10.5 kb from the SCN4A and GH1 genes, respectively.
Nakazato
S
S
Department of Molecular Biology, School of Medicine Kitasato University, Kanagawa, Japan.
Nomoto
K
K
Kazahari
K
K
Ono
M
M
D000595:Amino Acid Sequence; D000818:Animals; D015703:Antigens, CD / Q000737:chemistry / Q000235:genetics*; D001483:Base Sequence; D051925:CD79 Antigens; D002874:Chromosome Mapping; D004247:DNA; D015870:Gene Expression; D008040:Genetic Linkage; D013006:Growth Hormone / Q000235:genetics*; D019382:Human Growth Hormone / Q000235:genetics*; D006801:Humans; D051379:Mice; D008969:Molecular Sequence Data; D018482:Muscle, Skeletal / Q000378:metabolism*; D016133:Polymerase Chain Reaction; D051381:Rats; D017208:Rats, Wistar; D016415:Sequence Alignment; D015222:Sodium Channels / Q000235:genetics*
D015703:Antigens, CD; D051925:CD79 Antigens; C497883:CD79B protein, human; C497884:Cd79b protein, mouse; C497885:Cd79b protein, rat; D015222:Sodium Channels; D019382:Human Growth Hormone; D013006:Growth Hormone; D004247:DNA