title: Inhibition of lactogenic activities of bovine mammary gland explants by the whey fraction of bovine milk.
abstract: The regulation of milk constituents, synthesis and secretion in tissue cultures of the bovine mammary gland was altered by a whey fraction of bovine milk. alpha-Casein gene expression, casein secretion and fatty acid synthesis were inhibited by the whey fraction in a dose-dependent manner. The whey fraction inhibited the enhancement activity of prolactin on alpha-casein gene expression and fatty acid synthesis, and also inhibited casein secretion to the medium, in explants cultured in a medium with or without prolactin. No effect on the expression of the beta-lactoglobulin gene was found.
authors.affiliation: Institute of Animal Science, Volcani Center, Bet Dagan, Israel.
authors.forename: A
authors.initials: A
authors.lastname: Shamay
authors.forename: D A
authors.initials: DA
authors.lastname: Shinder
authors.forename: I
authors.initials: I
authors.lastname: Bruckental
authors.forename: N
authors.initials: N
authors.lastname: Silanikove
mesh_terms: D000818:Animals; D002364:Caseins / Q000096:biosynthesis; D002417:Cattle; D005227:Fatty Acids / Q000378:metabolism; D005260:Female; D005786:Gene Expression Regulation / Q000187:drug effects; D007774:Lactation / Q000502:physiology*; D007782:Lactoglobulins / Q000096:biosynthesis; D008321:Mammary Glands, Animal / Q000187:drug effects / Q000502:physiology*; D008892:Milk; D008894:Milk Proteins / Q000096:biosynthesis* / Q000494:pharmacology*; D009924:Organ Culture Techniques; D011388:Prolactin / Q000096:biosynthesis; D000067816:Whey Proteins
chemical_list: D002364:Caseins; D005227:Fatty Acids; D007782:Lactoglobulins; D008894:Milk Proteins; D000067816:Whey Proteins; D011388:Prolactin