Nipradilol inhibits DNA synthesis by regulating nitric oxide synthesis in cultured rat mesangial cells.
To elucidate whether nipradilol modulates mesangial cell function, we assessed the effects of nipradilol on DNA synthesis in the presence and absence of N-nitro-L-arginine methyl ester (L-NAME) in cultured rat mesangial cells stimulated with 1% fetal bovine serum. Nipradilol inhibited [3H]thymidine incorporation into mesangial cells in a dose- and time-dependent manner. In addition, the anti-mitogenic effect of 100 microM nipradilol was significantly inhibited in the presence of 10 microM L-NAME. Moreover, nipradilol increased intracellular cyclic guanosine monophosphate (cGMP). These results suggest that nipradilol exerts its efficacy in the treatment of several types of glomerulonephritis with mesangial cell proliferation by increasing in intracellular cGMP.
Nitta
K
K
Department of Medicine, Kidney Center, Tokyo Women's Medical College, Japan.
Tsutsui
T
T
Uchida
K
K
Eto
Y
Y
Natori
K
K
Honda
K
K
Yumura
W
W
Nihei
H
H
D000319:Adrenergic beta-Antagonists / Q000494:pharmacology*; D000818:Animals; D002417:Cattle; D002478:Cells, Cultured; D004261:DNA Replication / Q000187:drug effects*; D004791:Enzyme Inhibitors / Q000494:pharmacology; D005920:Glomerular Mesangium / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D019331:NG-Nitroarginine Methyl Ester / Q000494:pharmacology; D009569:Nitric Oxide / Q000096:biosynthesis*; D019001:Nitric Oxide Synthase / Q000037:antagonists & inhibitors; D011412:Propanolamines / Q000494:pharmacology*; D051381:Rats; D013936:Thymidine / Q000378:metabolism
D000319:Adrenergic beta-Antagonists; D004791:Enzyme Inhibitors; D011412:Propanolamines; D009569:Nitric Oxide; D019001:Nitric Oxide Synthase; C036089:nipradilol; D019331:NG-Nitroarginine Methyl Ester; D013936:Thymidine