title: [Restriction endonuclease Sse9I from Sporosarcina sp. strain 9D recognizes the 5'-AATT-3' DNA sequence].
abstract: A new restriction endonuclease Sse9I was isolated from the bacterial strain Sporosarcina sp. 9D. The enzyme belongs to Type II restrictases and recognizes the tetranucleotide sequence 5'-AATT-3'. The enzyme cleaves DNA before the first adenine residue, so it is a true isoschizomer of Tsp509I restrictase. However, unlike the prototype, Sse9I digests DNA at 55 degrees C and loses its activity after 20 min storage at 65 degrees C.
authors.affiliation: SibEnzyme, Novosibirsk, Russia.
authors.forename: D A
authors.initials: DA
authors.lastname: Gonchar
authors.forename: V S
authors.initials: VS
authors.lastname: Dedkov
authors.forename: V A
authors.initials: VA
authors.lastname: Verkhozina
authors.forename: Iu S
authors.initials: IuS
authors.lastname: Kusner
authors.forename: A V
authors.initials: AV
authors.lastname: Shevchenko
authors.forename: S Kh
authors.initials: SKh
authors.lastname: Degtiarev
mesh_terms: D004247:DNA / Q000378:metabolism*; D016936:Gram-Positive Endospore-Forming Bacteria / Q000201:enzymology*; D006358:Hot Temperature; D006868:Hydrolysis; D015265:Site-Specific DNA-Methyltransferase (Adenine-Specific) / Q000037:antagonists & inhibitors / Q000378:metabolism*; D013379:Substrate Specificity
chemical_list: D004247:DNA; C100875:DNA methyltransferase SSe9I; D015265:Site-Specific DNA-Methyltransferase (Adenine-Specific)