Assessment of immunization response to canine distemper virus vaccination in puppies using a clinic-based enzyme-linked immunosorbant assay.
To study the response to vaccination, an enzyme-linked immunosorbant assay (ELISA) immunoblot method was developed and tested to assay canine distemper virus (CDV) IgG antibody in puppies and compared to a standard virus neutralization (VN) test (r2 = 0.748). Ten litters of four puppies each were used in a vaccination study. Seventy-six percent of vaccinated puppies immunized with a modified live vaccine were successfully protected against CDV at 6 weeks of age. One puppy remained seronegative after vaccination at 6 and 9 weeks of age. This is the first report of vaccination responses of puppies to CDV using an in-clinic test kit based on solid-phase immunoassay technology.
Waner
T
T
Israel Institute for Biological Research, Ness Ziona, Israel.
Naveh
A
A
Ben Meir
N S
NS
Babichev
Z
Z
Carmichael
L E
LE
D000818:Animals; D000914:Antibodies, Viral / Q000097:blood*; D000917:Antibody Formation; D004216:Distemper / Q000276:immunology / Q000517:prevention & control*; D004285:Dogs; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D007112:Immunity, Maternally-Acquired / Q000276:immunology; D007114:Immunization / Q000662:veterinary; D007074:Immunoglobulin G / Q000097:blood; D009500:Neutralization Tests; D011247:Pregnancy; D014765:Viral Vaccines
D000914:Antibodies, Viral; D007074:Immunoglobulin G; D014765:Viral Vaccines