title: Specificity of milk peptide utilization by Lactococcus lactis.
abstract: To study the substrate specificity of the oligopeptide transport system of Lactococcus lactis for its natural substrates, the growth of L. lactis MG1363 was studied in a chemically defined medium containing milk peptides or a tryptic digest of alpha s2-casein as the source of amino acids. Peptides were separated into acidic, neutral, and basic pools by solid-phase extraction or by cation-exchange liquid chromatogrpaphy. Their ability to sustain growth and the time course of their utilization demonstrated the preferential use of hydrophobic basic peptides with molecular masses ranging between 600 and 1,100 Da by L. lactis MG1363 and the inability to use large, acidic peptides. These peptide utilization preferences reflect the substrate specificity of the oligopeptide transport system of the strain, since no significant cell lysis was inferred. Considering the free amino acid content of milk and these findings on peptide utilization, it was demonstrated that the cessation of growth of L. lactis MG1363 in milk was due to deprivation of leucine and methionine.
authors.affiliation: Unité de Recherches Laitières et Génétique Appliquée, Centre de Recherches de Jouy-en-Josas, France. juillard@jouy.inra.fr
authors.forename: V
authors.initials: V
authors.lastname: Juillard
authors.forename: A
authors.initials: A
authors.lastname: Guillot
authors.forename: D
authors.initials: D
authors.lastname: Le Bars
authors.forename: J C
authors.initials: JC
authors.lastname: Gripon
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001693:Biological Transport, Active; D002364:Caseins / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism; D007700:Kinetics; D013294:Lactococcus lactis / Q000254:growth & development / Q000378:metabolism*; D008892:Milk / Q000382:microbiology*; D008894:Milk Proteins / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism*; D008969:Molecular Sequence Data; D008970:Molecular Weight; D009842:Oligopeptides / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism*; D014357:Trypsin
chemical_list: D002364:Caseins; D008894:Milk Proteins; D009842:Oligopeptides; D014357:Trypsin