title: Presence of peptide synthetase gene transcripts and accumulation of ergopeptines in Claviceps purpurea and Neotyphodium coenophialum.
abstract: The production of toxic ergopeptine alkaloids by the fungi Claviceps purpurea and Neotyphodium coenophialum involves the activity of one or more nonribosomal peptide synthetases. Claviceps purpurea and N. coenophialum each have several different peptide synthetase genes, fragments of which have been cloned previously. An additional Claviceps purpurea peptide synthetase gene was cloned by hydridization with one of the N. coenophialum peptide synthetase gene fragments. We detected the presence of mRNA from the peptide synthetase genes in cultures of different ages grown under conditions favorable or unfavorable for ergopeptine production. All four peptide synthetase genes from Claviceps purpurea were transcribed under at least some of the experimental conditions. Transcripts from three of the four genes were detected under conditions consistent with their potential involvement in ergopeptine biosynthesis. All three peptide synthetase genes previously identified in N. coenophialum were transcribed during symbiotic growth of this fungus with tall fescue, as well as ergopeptine-producing cultures. The data show that all of the peptide synthetase genes are transcribed, that one of the peptide synthetase genes is dissociated from ergopeptine biosynthesis, and, as a result, prioritize the remaining genes for functional analyses by transformation-mediated gene disruption.
authors.affiliation: Division of Plant and Soil Sciences, West Virginia University, Morgantown, WV 26506-6057, USA.
authors.forename: S L
authors.initials: SL
authors.lastname: Annis
authors.forename: D G
authors.initials: DG
authors.lastname: Panaccione
mesh_terms: D002967:Claviceps / Q000201:enzymology / Q000235:genetics*; D003001:Cloning, Molecular; D004878:Ergotamine / Q000378:metabolism*; D006999:Hypocreales / Q000201:enzymology / Q000235:genetics*; D008969:Molecular Sequence Data; D009842:Oligopeptides / Q000378:metabolism*; D010453:Peptide Synthases / Q000235:genetics*; D006109:Poaceae / Q000382:microbiology; D012331:RNA, Fungal / Q000302:isolation & purification; D012333:RNA, Messenger / Q000302:isolation & purification; D013559:Symbiosis
chemical_list: D009842:Oligopeptides; D012331:RNA, Fungal; D012333:RNA, Messenger; D010453:Peptide Synthases; D004878:Ergotamine