Chiral and nonchiral determination of ketoprofen in pharmaceuticals by capillary zone electrophoresis.
The new method for the enantiomeric resolution of various 2-arylpropionic acids by capillary zone electrophoresis (CZE) using heptakis-tri-O-methyl-beta-cyclodextrin as chiral selector was applied to the determination of ketoprofen in different commercially-available pharmaceutical preparations. The analyte was determined under chiral and nonchiral conditions (viz. in the presence and absence of 50 mM heptakis-tri-O-methyl-beta-cyclodextrin in the background electrolyte), with significantly similar results and relative standard deviations from 1.2 to 6.5% in both cases. The limits of detection and determination for the inactive enantiomer, R-(-)-ketoprofen, were calculated to be 7.0 x 10(-7) and 1.6 x 10(-6) M, respectively. The proposed method was successfully used to determine enantiomeric purity in the drugs studied, with results comparable to those provided by the chiral HPLC method.
Blanco
M
M
Departament de Química, Facultat de Ciències, Universitat Autònoma de Barcelona, Bellaterra, Spain.
Coello
J
J
Iturriaga
H
H
Maspoch
S
S
Pérez-Maseda
C
C
D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000032:analysis*; D019075:Electrophoresis, Capillary / Q000379:methods*; D007660:Ketoprofen / Q000032:analysis*; D004364:Pharmaceutical Preparations / Q000737:chemistry*; D013237:Stereoisomerism
D000894:Anti-Inflammatory Agents, Non-Steroidal; D004364:Pharmaceutical Preparations; D007660:Ketoprofen