Analysis of minocycline by high-performance liquid chromatography in tissue and serum.
A sensitive and rapid reversed-phase high-performance liquid chromatography assay can be used to accurately determine serum and tissue minocycline concentrations. Minocycline is a broad spectrum tetracycline derivative with many applications. Tissue and serum samples were obtained from guinea pigs that had received either topical or intravenous minocycline. Samples were extracted using a Sep-Pak C18 cartridge and were injected into a microBondapak C18 column with an isocratic methanol mobile phase. Samples were analyzed using UV detection and produced sharp peaks with a retention time of 2.5 min. The lower limit of detection was 100 ng and drug recovery was 61%. This method greatly facilitated the analysis of minocycline while allowing for sensitivity.
Wrightson
W R
WR
Department of Surgery, University of Louisville School of Medicine, KY 40292, USA.
Myers
S R
SR
Galandiuk
S
S
D000287:Administration, Topical; D000818:Animals; D000900:Anti-Bacterial Agents / Q000008:administration & dosage / Q000032:analysis* / Q000097:blood; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D006168:Guinea Pigs; D007275:Injections, Intravenous; D008297:Male; D008911:Minocycline / Q000008:administration & dosage / Q000032:analysis* / Q000097:blood; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013056:Spectrophotometry, Ultraviolet; D014947:Wounds and Injuries
D000900:Anti-Bacterial Agents; D008911:Minocycline