Near-field fluorescence microscopy of cells.
We demonstrate the surface sensitivity of near-field scanning optical microscopy by fluorescence imaging of membrane and bulk proteins in cells. We discuss instrument design considerations for successful cell-biology work with NSOM and show that the technique is most suited for studying membrane proteins.
Enderle
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Molecular Design Institute, Materials Sciences Division, Lawrence Berkeley National Laboratory, Berkeley, CA 94720, USA.
Ha
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Chemla
D S
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Weiss
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D000818:Animals; D001412:Bacillus subtilis / Q000737:chemistry; D001426:Bacterial Proteins / Q000032:analysis*; D004910:Erythrocyte Membrane / Q000737:chemistry* / Q000469:parasitology / Q000648:ultrastructure; D049452:Green Fluorescent Proteins; D007202:Indicators and Reagents; D008164:Luminescent Proteins; D008565:Membrane Proteins / Q000032:analysis*; D018613:Microscopy, Confocal; D008855:Microscopy, Electron, Scanning; D008856:Microscopy, Fluorescence / Q000295:instrumentation* / Q000379:methods*; D055095:Optics and Photonics; D010963:Plasmodium falciparum; D015800:Protozoan Proteins / Q000032:analysis*; D012680:Sensitivity and Specificity; D013171:Spores, Bacterial / Q000737:chemistry / Q000648:ultrastructure
D001426:Bacterial Proteins; D007202:Indicators and Reagents; D008164:Luminescent Proteins; D008565:Membrane Proteins; D015800:Protozoan Proteins; D049452:Green Fluorescent Proteins