Evidence of the existence of alpha 1B-adrenoceptors on hepatocytes of domestic fowl (Gallus domesticus).
In liver cell membranes of laying hens alpha 1-adrenergic receptors were characterized using 3H-prazosin. Specific binding was saturable and indicative of a single class of high-affinity binding sites with a KD of 0.40 +/- 16 nM and a Bmax of 390 +/- 38 fmol/mg membrane protein. Preincubation of membranes with chlorethylclonidine significantly diminished the binding of 3H-prazosin (Bmax = 99 +/- 14 fmol/mg membrane protein). Furthermore, competition experiments revealed a rank order of prazosin >> phentolamine = oxymetazoline >> WB 4101, with Ki values of 0.71 +/- 0.62, 20 +/- 6, 32 +/- 13, and 119 +/- 48 nM, respectively. These results suggest the existence of alpha 1B-adrenergic receptors in hen liver cell membranes.
Neubert
E
E
Department of Veterinary Biochemistry, Faculty of Veterinary Medicine, University of Leipzig, Germany.
Gottschalk
J
J
Grün
E
E
Gürtler
H
H
D000818:Animals; D001667:Binding, Competitive; D002462:Cell Membrane / Q000378:metabolism; D002645:Chickens; D005260:Female; D008099:Liver / Q000378:metabolism*; D010058:Oviposition; D011224:Prazosin / Q000378:metabolism; D018340:Receptors, Adrenergic, alpha-1 / Q000378:metabolism*
D018340:Receptors, Adrenergic, alpha-1; D011224:Prazosin