title: Human choriocarcinoma JAR cells constitutively express pro-interleukin-1beta that can be released with Fc(gamma) receptor engagement.
abstract: Some authors have suggested that fetally derived syncytiotrophoblasts, which form the barrier between mother and the fetus, are an integral part of a complex macrophage-cytokine network involving maternal leukocytes, decidual cells, placental tissues, and even the fetus itself. We report here that syncytiotrophoblast-like JAR cells, a human choriocarcinoma cell line, share another feature common to cells of the monocyte-macrophage lineage, the ability to secrete IL-1beta when stimulated through their Fc(gamma) receptors. We incubated JAR cells with physiologically relevant concentrations of model BSA-rabbit IgG-anti-BSA immune complexes or monomeric rabbit IgG for periods of up to 72 h. Both monomeric IgG and immune complexes induced IL-1beta from JAR cells, although levels produced by immune complexes were approximately twice those induced by monomeric IgG. IL-1beta secretion was not inhibited by cycloheximide, and Western blots of JAR cell lysates using pro-IL-1beta MAb revealed constitutive expression of a 31-kD protein, whose levels declined within 2 h of stimulation by either IgG or immune complexes, but returned to baseline within 18 h.
authors.affiliation: Division of Immunology/Rheumatology, Wayne State University, and the Children's Hospital of Michigan, Detroit 48201, USA.
authors.forename: J N
authors.initials: JN
authors.lastname: Jarvis
authors.forename: C
authors.initials: C
authors.lastname: Xu
authors.forename: L
authors.initials: L
authors.lastname: Zhao
authors.forename: M
authors.initials: M
authors.lastname: Iobidze
authors.forename: H T
authors.initials: HT
authors.lastname: Moore
mesh_terms: D000818:Animals; D000936:Antigen-Antibody Complex / Q000276:immunology; D002822:Choriocarcinoma / Q000378:metabolism*; D003513:Cycloheximide / Q000494:pharmacology; D005260:Female; D006801:Humans; D007074:Immunoglobulin G / Q000276:immunology; D007375:Interleukin-1 / Q000096:biosynthesis* / Q000378:metabolism; D008180:Lupus Erythematosus, Systemic / Q000276:immunology; D008431:Maternal-Fetal Exchange / Q000276:immunology; D011247:Pregnancy; D011498:Protein Precursors / Q000096:biosynthesis* / Q000378:metabolism; D011500:Protein Synthesis Inhibitors / Q000494:pharmacology; D011817:Rabbits; D017452:Receptors, IgG / Q000276:immunology / Q000378:metabolism*; D012710:Serum Albumin, Bovine / Q000276:immunology; D014407:Tumor Cells, Cultured
chemical_list: D000936:Antigen-Antibody Complex; D007074:Immunoglobulin G; D007375:Interleukin-1; D011498:Protein Precursors; D011500:Protein Synthesis Inhibitors; D017452:Receptors, IgG; C043830:interleukin 1 precursor; D012710:Serum Albumin, Bovine; D003513:Cycloheximide