Localization and changes in distribution of brain alpha 2- and beta-adrenoceptors in response to acclimation state in the American bullfrog (Rana catesbeiana).
Alpha (alpha)- and beta (beta)-adrenoceptors regulate physiological processes in vertebrates. This study determined the location of alpha 2- and beta-adrenoceptors in the brain of the American bullfrog, Rana catesbeiana, using autoradiography. As the density of receptors may be affected by environmental temperature, a comparative numerical analysis of adrenoceptors in the areas of localization with respect to warm and cold acclimation was also carried out. Areas of greatest concentration of alpha 2-adrenoceptors were the accessory olfactory bulb, medial pallium, and olfactory bulb. Adrenoceptor numbers were significantly decreased in the accessory olfactory bulb and medial pallium in cold-acclimated animals. beta-adrenoceptors were localized in the thalamus, cerebellum, medial pallium, and amygdala/ striatum. Cold acclimation decreased adrenoceptor density in medial pallium and torus semicircularis and increased adrenoceptor density in the thalamus and hypothalamic preoptic areas. Among the alpha 2- and beta-adrenoceptors, only four regions of overlap existed, the medial pallium, hypothalamic preoptic area, optic tract, and isthmic tegmentum. Otherwise, where there were alpha 2-adrenoceptors, there were few or no beta-adrenoceptors. No alpha 2- or beta-adrenoceptors were found in the pituitary and optic chiasm. The distribution of adrenoceptors in particular areas of the brain may have functional significance with respect to physiological changes which occur in response to hibernation.
Bachman
G L
GL
Department of Biology, New Mexico State University, Las Cruces, New Mexico 88003, USA.
Uhlén
S
S
Herman
C A
CA
D000064:Acclimatization / Q000502:physiology*; D000317:Adrenergic alpha-Antagonists / Q000378:metabolism; D000818:Animals; D001345:Autoradiography; D001921:Brain / Q000033:anatomy & histology; D001923:Brain Chemistry / Q000502:physiology*; D005260:Female; D019329:Idazoxan / Q000031:analogs & derivatives / Q000378:metabolism; D008297:Male; D011892:Rana catesbeiana / Q000502:physiology*; D018341:Receptors, Adrenergic, alpha-2 / Q000378:metabolism*; D011943:Receptors, Adrenergic, beta / Q000378:metabolism*; D013696:Temperature
D000317:Adrenergic alpha-Antagonists; D018341:Receptors, Adrenergic, alpha-2; D011943:Receptors, Adrenergic, beta; C058525:2-methoxyidazoxan; D019329:Idazoxan