title: Stimulation of replication efficiency of a chromatin template by chromosomal protein HMG-17.
abstract: The effect of chromosomal protein HMG-17 on the replication of a chromatin template was studied with minichromosomes containing the SV40 origin of replication. The minichromosomes were assembled from M13 DNA in Xenopus egg extracts in either the absence or presence of HMG-17. Structural data show that HMG-17 was efficiently incorporated into the chromatin and induced an extended chromatin structure. Using an in vitro SV40 replication system, we find that minichromosomes containing HMG-17 replicate with higher efficiency than minichromosomes deficient of HMG-17. The replicational potential of chromatin was enhanced only when HMG-17 was incorporated into the template during, but not after, chromatin assembly. HMG-17 stimulated replication only from a chromatin template, but not from protein-free DNA. Thus, HMG-17 protein enhances the rate of replication of a chromatin template by unfolding the higher order chromatin structure and increasing the accessibility of target sequences to components of the replication machinery.
authors.affiliation: University of Konstanz, Division of Biology, Universitätsstr.10, 78457 Konstanz, Federal Republic of Germany.
authors.forename: B
authors.initials: B
authors.lastname: Vestner
authors.forename: M
authors.initials: M
authors.lastname: Bustin
authors.forename: C
authors.initials: C
authors.lastname: Gruss
mesh_terms: D000818:Animals; D017104:Bacteriophage M13 / Q000235:genetics; D002843:Chromatin / Q000235:genetics* / Q000378:metabolism; D002875:Chromosomes / Q000502:physiology; D004261:DNA Replication; D004278:DNA, Superhelical / Q000096:biosynthesis; D004279:DNA, Viral / Q000378:metabolism; D005260:Female; D006609:High Mobility Group Proteins / Q000378:metabolism*; D007700:Kinetics; D009865:Oocytes / Q000502:physiology; D015183:Restriction Mapping; D013698:Templates, Genetic; D014982:Xenopus laevis
chemical_list: D002843:Chromatin; D004278:DNA, Superhelical; D004279:DNA, Viral; D006609:High Mobility Group Proteins