title: CD26 (dipeptidyl peptidase i.v.) on human T lymphocytes does not mediate adhesion of these cells to endothelial cells or fibroblasts.
abstract: We have examined the role of CD26 (dipeptidyl peptidase IV) in the adhesion of resting and activated T lymphocytes to endothelial cells and fibroblasts. For this purpose, we ran a short-time adhesion assay under different strategies: Adhesion of T lymphocytes was determined in the presence of different anti-CD26 monoclonal antibodies, or in the presence of synthetic inhibitors of the enzymatic function of CD26. In addition, the expression of CD26 on T lymphocytes, which were adherent to endothelial cells or fibroblasts, was performed by flow cytometric analysis. We found that the anti-CD26 monoclonal antibodies tested here were not able to inhibit T cell adhesion to monolayers of endothelial cells or fibroblasts. Secondly, synthetic inhibitors of the enzymatic function of CD26 had no effect on the adhesion of T lymphocytes to endothelial cells or fibroblasts. Furthermore, CD26-positive T cells were not accumulated in the adherent population. These results suggest that CD26 on T lymphocytes plays no role in T cell adhesion to endothelial cells or fibroblasts.
authors.affiliation: Forschungszentrum Borstel, Department of Immunology and Cell Biology, Germany.
authors.forename: T
authors.initials: T
authors.lastname: Mattern
authors.forename: C
authors.initials: C
authors.lastname: Reich
authors.forename: U
authors.initials: U
authors.lastname: Schönbeck
authors.forename: S
authors.initials: S
authors.lastname: Ansorge
authors.forename: H U
authors.initials: HU
authors.lastname: Demuth
authors.forename: H
authors.initials: H
authors.lastname: Loppnow
authors.forename: A J
authors.initials: AJ
authors.lastname: Ulmer
authors.forename: H D
authors.initials: HD
authors.lastname: Flad
mesh_terms: D018819:Dipeptidyl Peptidase 4 / Q000276:immunology / Q000502:physiology*; D004730:Endothelium, Vascular / Q000276:immunology / Q000502:physiology*; D005347:Fibroblasts / Q000276:immunology / Q000502:physiology*; D006801:Humans; D013601:T-Lymphocytes / Q000201:enzymology / Q000276:immunology / Q000502:physiology*
chemical_list: D018819:Dipeptidyl Peptidase 4