title: Inhibition of the specific 3H-DL-Glu binding in the hippocampus of rat brain by lead.
abstract: The effect of Pb2+ on 3H-DL-Glutamate (3H-DL-Glu) binding in the membrane preparations from the hippocampus of rat brain was investigated with a view to explaining the cognitive and learning deficits produced by the heavy metal. The results indicated that Pb2+ (3.1-25.0 mumol.L-1) inhibited 3H-DL-Glu binding in a concentration-dependent manner. Scatchard analysis further revealed that at a concentration of 6.3 mumol.L-1 Pb2+ interfered with binding mainly through significantly decreasing the density of binding sites. This finding provided an important insight into Pb2(+)-induced impairments in learning and memory previously documented in children and in experimental animals chronically exposed to Pb2+.
authors.affiliation: Department of Toxicology, Chinese Academy of Preventive Medicine, Beijing, China.
authors.forename: H S
authors.initials: HS
authors.lastname: Zhang
authors.forename: X L
authors.initials: XL
authors.lastname: Zhao
authors.forename: L H
authors.initials: LH
authors.lastname: Song
authors.forename: Y H
authors.initials: YH
authors.lastname: Qin
mesh_terms: D000818:Animals; D001665:Binding Sites / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D005973:Glutamine / Q000378:metabolism*; D006624:Hippocampus / Q000187:drug effects / Q000378:metabolism*; D007854:Lead / Q000633:toxicity*; D007858:Learning / Q000187:drug effects; D008297:Male; D008568:Memory / Q000187:drug effects; D009942:Organometallic Compounds / Q000008:administration & dosage / Q000633:toxicity*; D051381:Rats; D017208:Rats, Wistar; D013237:Stereoisomerism; D014316:Tritium
chemical_list: D009942:Organometallic Compounds; D005973:Glutamine; D014316:Tritium; D007854:Lead; C008261:lead acetate