title: Skeletal muscle myosin monomer in equilibrium with filaments forms a folded conformation.
abstract: Rabbit skeletal myosin forms stable filaments under physiological conditions, and only a small amount stays as a monomer in equilibrium with filaments. The myosin monomers were observed in two conformational states, as extended and folded forms upon electron microscopy and gel filtration high performance liquid chromatography. The fraction of monomers in the folded conformation increased with a decrease in the concentration of NaCl below 0.2 M, and the conformational state was affected neither by the presence of ATP nor by the phosphorylation of regulatory light chain. In most of the folded monomers, the tail bent back toward the heads at one region, 45 nm apart from the head-tail junction, and the remaining tail portion containing the C-terminal tip appeared to interact with the head-tail junction. Only a small percentage of the folded monomers was in a more compact conformation close to the 10 S conformation of vertebrate smooth muscle and non-muscle myosins. The folded monomers, however, may not trap the products of ATP hydrolysis as assessed by single turnover experiments. The percentage of monomers in the 10 S-like conformation was increased by the exchange of a regulatory light chain with the smooth muscle light chain, indicating the participation of head-tail junction, including the regulatory light chain in the formation of folded conformation. The folded conformation may be common to various myosin IIs, suggestive of common roles for the folded monomers.
authors.affiliation: Division of Chemistry, Graduate School of Science, Hokkaido University, Sapporo 060, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Katoh
authors.forename: K
authors.initials: K
authors.lastname: Konishi
authors.forename: M
authors.initials: M
authors.lastname: Yazawa
mesh_terms: D000818:Animals; D008854:Microscopy, Electron; D018482:Muscle, Skeletal / Q000648:ultrastructure*; D009130:Muscle, Smooth / Q000648:ultrastructure; D009218:Myosins / Q000648:ultrastructure*; D009994:Osmolar Concentration; D011487:Protein Conformation; D011817:Rabbits; D013552:Swine
chemical_list: D009218:Myosins