title: Heterobinary adhesins based on the Escherichia coli FimH fimbrial protein.
abstract: The FimH adhesin of Escherichia coli type 1 fimbriae confers the ability to bind to D-mannosides by virtue of a receptor-binding domain located in its N-terminal region. This protein was engineered into a heterobifunctional adhesin by introducing a secondary binding site in the C-terminal region. The insertion of histidine clusters into this site resulted in coordination of various metal ions by recombinant cells expressing chimeric FimH proteins. In addition, libraries consisting of random peptide sequences inserted into the FimH display system and screened by a "panning" technique were used to identify specific sequences conferring the ability to adhere to Ni2+ and Cu2+. Recombinant cells expressing heterobifunctional FimH adhesins could adhere simultaneously to both metals and saccharides. Finally, combining the metal-binding modifications with alterations in the natural receptor-binding region demonstrated the ability to independently modulate the binding of FimH to two ligands simultaneously.
authors.affiliation: Department of Microbiology, Technical University of Denmark, Lyngby, Denmark.
authors.forename: M A
authors.initials: MA
authors.lastname: Schembri
authors.forename: P
authors.initials: P
authors.lastname: Klemm
mesh_terms: D018829:Adhesins, Bacterial / Q000737:chemistry / Q000235:genetics / Q000502:physiology*; D018830:Adhesins, Escherichia coli; D000595:Amino Acid Sequence; D001422:Bacterial Adhesion; D001665:Binding Sites; D003300:Copper / Q000378:metabolism; D004926:Escherichia coli / Q000737:chemistry*; D036781:Fimbriae Proteins; D008358:Mannose / Q000378:metabolism; D008969:Molecular Sequence Data; D009532:Nickel / Q000378:metabolism; D015202:Protein Engineering
chemical_list: D018829:Adhesins, Bacterial; D018830:Adhesins, Escherichia coli; C058769:fimH protein, E coli; D036781:Fimbriae Proteins; D003300:Copper; D009532:Nickel; D008358:Mannose