title: Rapid method for isolation of total RNA from eukaryotic cell lines and leukocytes.
abstract: Total RNA was isolated from human leukocytes (monocytes, granulocytes), various cell lines (COS-7, Mono-Mac-6, L-132, HaCaT, EA.hy926, HL-60), and fungal mycelium by a rapid two-step method. Cells were lysed with NaDodSO4 in a citric acid-containing buffer. This procedure was succeeded by salt precipitation to remove contaminating DNA and protein and a final alcohol precipitation of RNA. Isolated RNA was of high quality, with a reasonable yield and little or no protein or DNA contamination. We present here a fast method for preparing RNA particularly from cell lines, which limits the use of toxic compounds.
authors.affiliation: Institute of Experimental Dermatology, University of Münster, Germany.
authors.forename: J
authors.initials: J
authors.lastname: Dreier
authors.forename: P
authors.initials: P
authors.lastname: Högger
authors.forename: C
authors.initials: C
authors.lastname: Sorg
mesh_terms: D000818:Animals; D019556:COS Cells; D002460:Cell Line; D005057:Eukaryotic Cells / Q000737:chemistry*; D005658:Fungi / Q000737:chemistry; D006801:Humans; D007962:Leukocytes / Q000737:chemistry*; D012313:RNA / Q000302:isolation & purification*
chemical_list: D012313:RNA