Peptides that mimic the group B streptococcal type III capsular polysaccharide antigen.
Microbial polysaccharides are notably poor immunogens. We have developed an alternate route for the production of Abs to important carbohydrate epitopes. mAb S9, a protective mAb against the type III capsular polysaccharide of group B streptococci (GBS), was used to select epitope analogues from a peptide display phage library. Depending upon desorption conditions, two populations of phage were identified with displayed sequences of WENWMMGNA and FDTGAFDPDWPA. ELISA results demonstrated that these phage bound to S9 and no other Abs. Phage blocked the binding of S9 to type III GBS, but did not block binding by another anti-GBS mAb. Phage displaying the latter peptide sequence showed greater inhibition. Ab S9 and other monoclonal and polyclonal anti-GBS type III antisera bound the synthetic peptide FDTGAFDPDWPAC. The binding of S9 to GBS was inhibited by the free peptide with an IC50 of 30 microg/ml. The binding of polyclonal anti-GBS antibodies to peptide could be blocked by intact GBS as well as purified capsular polysaccharide. The peptide was conjugated to three different carriers and was used to immunize mice. All mice produced a significant antibody response to GBS and to the purified capsular polysaccharide following a single immunization. These data demonstrate that a peptide mimetic of the GBS capsular polysaccharide is both antigenic and immunogenic. The incorporation of such peptides into vaccine preparations may enhance the efficacy of vaccines in inducing Ab responses to important carbohydrate epitopes.
Pincus
S H
SH
Department of Microbiology, Montana State University, Bozeman 59717-3520, USA. spincus@montana.edu
Smith
M J
MJ
Jennings
H J
HJ
Burritt
J B
JB
Glee
P M
PM
D000595:Amino Acid Sequence; D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D000918:Antibody Specificity; D016667:Bacterial Capsules / Q000276:immunology*; D018604:Epitope Mapping; D051379:Mice; D019151:Peptide Library; D010455:Peptides / Q000276:immunology; D013292:Streptococcus agalactiae / Q000276:immunology*
D000911:Antibodies, Monoclonal; D019151:Peptide Library; D010455:Peptides