Capillary gas chromatographic analysis of serum bile acids as the n-butyl ester-trimethylsilyl ether derivatives.
Gas chromatographic separations of n-butyl ester-trimethylsilyl ether derivatives of several common bile acids were compared with those of the corresponding methyl ester-trimethylsilyl ether derivatives on a CP-Sil-5 CB capillary column. Both types of derivatives were similarly resolved from each other. However, the n-butyl ester-trimethylsilyl ether derivatives of the bile acids showed longer retention times than the corresponding methyl ester-trimethylsilyl ethers and unlike the methyl ester-trimethylsilyl ether derivatives, were completely resolved from and eluted later than the trimethylsilyl ethers of common plasma sterols including sitosterol. A simplified method of plasma work-up for quantitation of bile acids and application of the above method in quantification of plasma bile acids in humans is described.
Batta
A K
AK
Department of Medicine and the Liver Institute, UMDNJ-NJ Medical School, Newark, NJ, USA.
Salen
G
G
Rapole
K R
KR
Batta
M
M
Earnest
D
D
Alberts
D
D
D001647:Bile Acids and Salts / Q000097:blood* / Q000737:chemistry; D002849:Chromatography, Gas / Q000379:methods*; D004952:Esters / Q000737:chemistry; D004987:Ethers / Q000737:chemistry; D006801:Humans; D014297:Trimethylsilyl Compounds / Q000737:chemistry
D001647:Bile Acids and Salts; D004952:Esters; D004987:Ethers; D014297:Trimethylsilyl Compounds