title: Soluble intercellular adhesion molecule-1 in ovarian follicles: production by granulosa luteal cells and levels in follicular fluid.
abstract: OBJECTIVE
  To determine the concentration of the soluble form of intercellular adhesion molecule (ICAM)-1 in granulosa luteal cell-conditioned media and in follicular fluid (FF).
  
  
  METHODS
  Granulosa cells and FF samples were obtained at the time of oocyte retrieval for IVF. In 10 women, a total of 33 fluids were obtained from individual follicles, whereas in 70 women, the follicular aspirates were pooled.
  
  
  METHODS
  Clinica "L. Mangiagalli" and Reproductive Center, San Raffaele Hospital, Milan, Italy.
  
  
  METHODS
  Eighty women referred for IVF for tubal factor or male factor infertility.
  
  
  METHODS
  Women underwent ovarian hyperstimulation.
  
  
  METHODS
  Soluble ICAM-1 was measured by an ELISA, and its levels were correlated with follicular size, the number of retrieved oocytes, and the number of follicles with a diameter of >15 mm.
  
  
  RESULTS
  The concentration of soluble ICAM-1 in granulosa luteal cell-conditioned media was 17.8 +/- 1.8 ng/5 x 10(5) cells. Interleukin-1beta can stimulate soluble ICAM-1 release in a dose-dependent manner. A significant positive correlation was demonstrated between levels of soluble ICAM-1 in pooled FF and the number of retrieved oocytes or the number of follicles with a diameter of >15 mm.
  
  
  CONCLUSIONS
  Soluble ICAM-1 can be released by granulosa luteal cells and can be detected in FF after ovarian hyperstimulation. Levels of soluble ICAM-1 in FF correlate directly with some indices of ovarian function.
authors.affiliation: University of Milan, Italy.
authors.forename: P
authors.initials: P
authors.lastname: Viganò
authors.forename: F
authors.initials: F
authors.lastname: Fusi
authors.forename: B
authors.initials: B
authors.lastname: Gaffuri
authors.forename: V
authors.initials: V
authors.lastname: Bonzi
authors.forename: A
authors.initials: A
authors.lastname: Ferrari
authors.forename: M
authors.initials: M
authors.lastname: Vignali
mesh_terms: D002478:Cells, Cultured; D003470:Culture Media; D004305:Dose-Response Relationship, Drug; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D005434:Flow Cytometry; D015571:Follicular Fluid / Q000737:chemistry*; D006107:Granulosa Cells / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D006801:Humans; D018799:Intercellular Adhesion Molecule-1 / Q000032:analysis* / Q000096:biosynthesis; D007375:Interleukin-1 / Q000494:pharmacology; D016014:Linear Models; D008184:Luteal Cells / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*
chemical_list: D003470:Culture Media; D007375:Interleukin-1; D018799:Intercellular Adhesion Molecule-1