Processing of the Borna disease virus glycoprotein gp94 by the subtilisin-like endoprotease furin.
Open reading frame IV (ORF-IV) of Borna disease virus (BDV) encodes a protein with a calculated molecular mass of ca. 57 kDa (p57), which increases after N glycosylation to 94 kDa (gp94). The unglycosylated and glycosylated proteins are proteolytically cleaved by the subtilisin-like protease furin. Furin most likely recognizes one of three potential cleavage sites, namely, an arginine at position 249 of the ORF-IV gene product. The furin inhibitor decRVKRcmk decreases the production of infectious BDV significantly, indicating that proteolytic cleavage of the gp94 precursor molecule is necessary for the full biological activity of the BDV glycoprotein.
Richt
J A
JA
Institut für Virologie, Giessen, Germany. juergen.a.richt@vetmed.uni-giessen.de
Fürbringer
T
T
Koch
A
A
Pfeuffer
I
I
Herden
C
C
Bause-Niedrig
I
I
Garten
W
W
D000590:Amino Acid Chloromethyl Ketones / Q000494:pharmacology; D000595:Amino Acid Sequence; D000818:Animals; D001891:Borna disease virus / Q000378:metabolism* / Q000502:physiology; D001921:Brain / Q000378:metabolism; D002460:Cell Line; D002522:Chlorocebus aethiops; D045683:Furin; D006023:Glycoproteins / Q000737:chemistry / Q000378:metabolism*; D006031:Glycosylation; D011499:Protein Processing, Post-Translational; D051381:Rats; D015842:Serine Proteinase Inhibitors / Q000494:pharmacology; D013381:Subtilisins / Q000378:metabolism*; D014764:Viral Proteins / Q000737:chemistry / Q000378:metabolism*
D000590:Amino Acid Chloromethyl Ketones; D006023:Glycoproteins; D015842:Serine Proteinase Inhibitors; D014764:Viral Proteins; D013381:Subtilisins; D045683:Furin