title: A Ba(2+)-insensitive K+ conductance in the basolateral membrane of rabbit cortical thick ascending limb cells.
abstract: The nature of the K+ exit across the basolateral membrane of microperfused rabbit cortical thick ascending limbs (cTALs) was investigated using the transepithelial and transmembrane potential difference (PDte, PDbl) and conductance measurements. An increase in bath K+ concentration from 4 to 10, 25, 50 mmol/l depolarized the basolateral membrane in a concentration-dependent manner, accompanied by a decrease in the fractional resistance of the basolateral membrane (FRbl). The Cl- channel blocker, 5-nitro-2-(3-phenylpropyl-amino)-benzoic acid (NPPB), did not prevent these effects. The effect of Ba2+ on PDbl was bimodally distributed: paradoxically, in the tubules in which Ba2+ largely depolarized, the effects on PDbl of the bath K+ concentration increases were not inhibited by extracellular Ba2+, in tubules in which Ba2+ moderately depolarized, Ba2+ partially inhibited the K+ concentration increase-induced depolarization of the basolateral membrane. However, the parallel decrease in FRbl was Ba2+ insensitive, indicating that the K+ channel of the basolateral membrane was not modified by extracellular Ba2+. The Ba(2+)-induced depolarizations were prevented by furosemide suggesting that Ba2+ acts by inhibiting basolateral KCl extrusion. Finally, the K+ concentration increase-induced depolarizations were insensitive to tetraethylammonium, charybdotoxin, apamin and verapamil. In conclusion, the present study provides evidence that, in addition to a Ba(2+)-sensitive KCl cotransport system, the basolateral membrane of rabbit cTAL cells possesses a K+ conductance which is insensitive to extracellular Ba2+.
authors.affiliation: Département de Biologie cellulaire et moléculaire, CEA Saclay, Gif-sur-Yvette, France. stefano@dsvidf.cea.fr
authors.forename: A
authors.initials: A
authors.lastname: Di Stefano
authors.forename: R
authors.initials: R
authors.lastname: Greger
authors.forename: E
authors.initials: E
authors.lastname: Desfleurs
authors.forename: C
authors.initials: C
authors.lastname: de Rouffignac
authors.forename: M
authors.initials: M
authors.lastname: Wittner
mesh_terms: D000818:Animals; D001030:Apamin / Q000494:pharmacology; D001464:Barium / Q000494:pharmacology*; D002462:Cell Membrane / Q000378:metabolism*; D018999:Charybdotoxin / Q000494:pharmacology; D018118:Chloride Channels / Q000037:antagonists & inhibitors / Q000378:metabolism; D002712:Chlorides / Q000378:metabolism; D004553:Electric Conductivity; D005260:Female; D005665:Furosemide / Q000494:pharmacology; D066298:In Vitro Techniques; D017136:Ion Transport; D008138:Loop of Henle / Q000166:cytology / Q000378:metabolism*; D008564:Membrane Potentials / Q000502:physiology; D008954:Models, Biological; D009579:Nitrobenzoates / Q000494:pharmacology; D011188:Potassium / Q000378:metabolism*; D015221:Potassium Channels / Q000378:metabolism*; D011817:Rabbits; D012965:Sodium Chloride / Q000378:metabolism; D014700:Verapamil / Q000494:pharmacology
chemical_list: D018118:Chloride Channels; D002712:Chlorides; D009579:Nitrobenzoates; D015221:Potassium Channels; D018999:Charybdotoxin; D001030:Apamin; D001464:Barium; C058176:5-nitro-2-(3-phenylpropylamino)benzoic acid; D012965:Sodium Chloride; D005665:Furosemide; D014700:Verapamil; D011188:Potassium