Recombinant porcine follicle stimulating hormone produced in baculovirus-insect cells induces rat ovulation in vivo and gene expression of tissue plasminogen activator in vitro.
Superovulatory responses in cattle are known to be highly variable. In the present study, a recombinant porcine follicle stimulating hormone (rpFSH) produced in baculovirus-insect cells was utilised to evaluate the role of this recombinant FSH in control of the ovulatory process. Immature hypophysectomised rats were implanted with oestrogen pellet (10 mg diethylstilbestrol) and then primed with pregnant mare serum gonadotropin (PMSG, 17.5 IU, sc). Fifty-two hours later, 100 microg rpFSH or saline was injected (sc) to induce ovulation. All rats that received rpFSH ovulated with about eight ova rat(-1), whereas none of the control animals did. Ovulation induced by rpFSH was associated with an increase in the ovarian activity and message levels of tissue-type plasminogen activator (tPA), a protease important in the preovulatory degradation of the follicle wall. Furthermore, addition of rpFSH to the cultured rat granulosa cells resulted in a significant increase in tPA enzyme activity. These results demonstrate that rpFSH produced in baculovirus-insect cells has biological potency in ovulation as well as gene expression of tPA, providing a large advantage of this massive expression system in the reproduction of domestic animals.
Inaba
T
T
Department of Animal Reproduction, College of Agriculture, Osaka Prefecture University, Sakai, Japan.
Mori
J
J
Ohmura
M
M
Tani
H
H
Kato
Y
Y
Tomizawa
K
K
Kato
T
T
Ihara
T
T
Sato
I
I
Ueda
S
S
D000818:Animals; D016367:Baculoviridae; D002417:Cattle; D002460:Cell Line; D002478:Cells, Cultured; D004054:Diethylstilbestrol / Q000494:pharmacology; D005260:Female; D005640:Follicle Stimulating Hormone / Q000096:biosynthesis / Q000494:pharmacology*; D015971:Gene Expression Regulation, Enzymologic / Q000187:drug effects; D006064:Gonadotropins, Equine / Q000494:pharmacology*; D006107:Granulosa Cells / Q000166:cytology / Q000201:enzymology; D007016:Hypophysectomy; D010060:Ovulation / Q000187:drug effects*; D010062:Ovulation Induction / Q000379:methods / Q000662:veterinary*; D051381:Rats; D017207:Rats, Sprague-Dawley; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000494:pharmacology; D018411:Spodoptera; D013552:Swine; D010959:Tissue Plasminogen Activator / Q000096:biosynthesis*; D014162:Transfection
D006064:Gonadotropins, Equine; D011994:Recombinant Proteins; D004054:Diethylstilbestrol; D005640:Follicle Stimulating Hormone; D010959:Tissue Plasminogen Activator