A novel lectin from Sarcophaga. Its purification, characterization, and cDNA cloning.
A novel C-type lectin that agglutinates rabbit red cells was purified from NIH-Sape-4 cells derived from the flesh fly (Sarcophaga peregrina), and its cDNA was isolated. This lectin, named granulocytin, appeared to be a trimer of a 20-kDa subunit consisting of 151 amino acid residues. The gene for granulocytin was activated in third instar larvae, and its expression was enhanced when the larval body wall was injured. In third instar larvae, granulocytin was found to be synthesized by hemocytes and secreted into the hemolymph. The molecular mass and gene expression patterns of granulocytin were very similar to those of Drosophila lectin that we reported previously (Haq, S., Kubo, T., Kurata, S., Kobayashi, A., and Natori, S. (1996) J. Biol. Chem. 271, 20213-20218). However, these two lectins showed amino acid identities of 20% at most, and no significant hapten sugar for granulocytin was identified.
Fujita
Y
Y
Faculty of Pharmaceutical Sciences, University of Tokyo, Bunkyo-ku, Tokyo 113, Japan.
Kurata
S
S
Homma
K
K
Natori
S
S
D000273:Adipose Tissue / Q000378:metabolism; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002460:Cell Line; D002846:Chromatography, Affinity; D003001:Cloning, Molecular; D018076:DNA, Complementary; D004175:Diptera / Q000254:growth & development / Q000378:metabolism*; D004330:Drosophila; D004625:Embryo, Nonmammalian / Q000378:metabolism; D018507:Gene Expression Regulation, Developmental; D006386:Hemagglutination Tests; D006434:Hemocytes / Q000378:metabolism; D006458:Hemolymph / Q000378:metabolism; D019476:Insect Proteins; D007814:Larva; D037102:Lectins / Q000096:biosynthesis* / Q000737:chemistry* / Q000302:isolation & purification; D037181:Lectins, C-Type; D008969:Molecular Sequence Data; D011817:Rabbits; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000737:chemistry / Q000302:isolation & purification; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid
C043030:20kDa lectin protein, Sarcophaga; D018076:DNA, Complementary; D019476:Insect Proteins; D037102:Lectins; D037181:Lectins, C-Type; D011994:Recombinant Proteins