title: Cloning of the ribosomal protein L41 gene of Phaffia rhodozyma and its use a drug resistance marker for transformation.
abstract: The ribosomal protein L41 gene of Phaffia rhodozyma was cloned and used as a dominant selectable marker for cycloheximide resistance in the transformation of P. rhodozyma. Electrotransformation with a plasmid containing a ribosomal DNA fragment as a targeting signal typically yielded 800 to 1,200 transformants/microgram of DNA with an integrated copy number of about seven per haploid genome.
authors.affiliation: Applied Microbiology Research Division, Korea Research Institute of Bioscience and Biotechnology, Yusong, Taejon, Korea.
authors.forename: I G
authors.initials: IG
authors.lastname: Kim
authors.forename: S K
authors.initials: SK
authors.lastname: Nam
authors.forename: J H
authors.initials: JH
authors.lastname: Sohn
authors.forename: S K
authors.initials: SK
authors.lastname: Rhee
authors.forename: G H
authors.initials: GH
authors.lastname: AN
authors.forename: S H
authors.initials: SH
authors.lastname: Lee
authors.forename: E S
authors.initials: ES
authors.lastname: Choi
mesh_terms: D003001:Cloning, Molecular; D003513:Cycloheximide / Q000494:pharmacology*; D004352:Drug Resistance, Microbial; D005800:Genes, Fungal; D010957:Plasmids; D016133:Polymerase Chain Reaction; D012269:Ribosomal Proteins / Q000235:genetics*; D014170:Transformation, Genetic; D015003:Yeasts / Q000187:drug effects / Q000235:genetics*
chemical_list: D012269:Ribosomal Proteins; D003513:Cycloheximide