title: [PCR analysis of DNA in paraffin sections of malignant lymphomas].
abstract: The polymerase chain reaction was used to detect the clonal rearrangement of immunoglobulin heavy chain gene in paraffin embedded samples of human lymph nodes. We developed a sensitive and reliable method of the DNA isolation from 4-5 tissue sections, which enabled us to perform 50-100 PCR reactions. We compared the reactive lymph nodes and non-Hodgkin's malignant lymphomas using framework 3 and J region primers. PCR products were examined by agarose gel electrophoresis. The dominant 80-120 bp amplification product was found in all lymphoma samples. The samples of reactive nodes were negative.
authors.affiliation: Ustav experimentální medicíny AV CR, Praha.
authors.forename: J
authors.initials: J
authors.lastname: Müller
authors.forename: R A
authors.initials: RA
authors.lastname: Dzhaborkhel
authors.forename: E
authors.initials: E
authors.lastname: Dvorák
authors.forename: V
authors.initials: V
authors.lastname: Mandys
authors.forename: B
authors.initials: B
authors.lastname: Bednár
mesh_terms: D004273:DNA, Neoplasm / Q000032:analysis*; D006801:Humans; D008228:Lymphoma, Non-Hodgkin / Q000235:genetics*; D016612:Paraffin Embedding; D016133:Polymerase Chain Reaction
chemical_list: D004273:DNA, Neoplasm