title: Genetic variation in some populations of the golden-striped salamander, Chioglossa lusitanica (Amphibia: Urodela), in Portugal.
abstract: Genetic variation in the golden-striped salamander (Chioglossa lusitanica) was assessed in 231 individuals from four Portuguese populations by means of horizontal starch gel electrophoresis and isoelectric focusing. Three of 19 enzyme systems, representing 21 presumptive loci, were found to be polymorphic: phosphoglucomutase 1 (PGM1), peptidase B (PEPB), and peptidase D (PEPD). The observed average heterozygosity in Chioglossa lusitanica (0.027) is significantly lower than that observed for other amphibians, either urodeles or salamandrids. Differences in allele frequencies and the presence of private alleles are indicative of a high degree of population differentiation. PEPD, in particular, seems to be a diagnostic locus separating the southernmost population studied from the others.
authors.affiliation: Departamento de Zoologia e Antropologia, Faculdade de Ciências, Universidade do Porto, Praça Gomes Teixeira, Portugal.
authors.forename: J
authors.initials: J
authors.lastname: Alexandrino
authors.forename: N
authors.initials: N
authors.lastname: Ferrand
authors.forename: J W
authors.initials: JW
authors.lastname: Arntzen
mesh_terms: D000626:Aminopeptidases / Q000235:genetics; D000818:Animals; D004150:Dipeptidases / Q000235:genetics; D004152:Dipeptidyl-Peptidases and Tripeptidyl-Peptidases; D004592:Electrophoresis, Starch Gel; D005787:Gene Frequency; D005843:Geography; D006579:Heterozygote; D007525:Isoelectric Focusing; D010733:Phosphoglucomutase / Q000235:genetics; D011110:Polymorphism, Genetic; D011174:Portugal; D014562:Caudata / Q000235:genetics*
chemical_list: D000626:Aminopeptidases; C026045:tripeptide aminopeptidase; D004150:Dipeptidases; C022449:proline dipeptidase; D004152:Dipeptidyl-Peptidases and Tripeptidyl-Peptidases; D010733:Phosphoglucomutase