title: Automated on-line dialysis for sample preparation and HPLC analysis of antidepressant drugs in human plasma. Inhibition of interaction with the dialysis membrane.
abstract: Antidepressant drugs interact with the dialysis membrane and were selected as model substances to study inhibition of analyte-membrane interactions. A chemometric approach based on response surface modelling was used for screening and optimisation of dialysis recoveries. Optimal dialysis recoveries (52-65%) were obtained for the model compounds (mianserine, amitriptyline, nortriptyline, imipramine and desimipramine) when a cationic surfactant added to the donor solution of the dialyser was used to inhibit analyte-membrane interactions. Automated analysis of antidepressants in plasma was performed by connecting the ASTED (Automated Sequential Trace Enrichment of Dialysates) system to high-performance liquid chromatography (HPLC). The drugs were detected by ultraviolet detection and fluorescence detection after post-column photochemical reaction. Validation of the method showed linear standard curves for all the drugs in the concentration range 50-2000 nmol 1-1. Within-and between day relative standard deviations ranged from 1.1 to 5.7%.
authors.affiliation: School of Pharmacy, University of Oslo, Norway Karianne.Johansen@farmasi.unio.no
authors.forename: K
authors.initials: K
authors.lastname: Johansen
authors.forename: K E
authors.initials: KE
authors.lastname: Rasmussen
mesh_terms: D000928:Antidepressive Agents / Q000097:blood*; D002482:Cellulose / Q000031:analogs & derivatives; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D003956:Dialysis; D006801:Humans; D008567:Membranes, Artificial; D013048:Specimen Handling / Q000379:methods; D013499:Surface Properties
chemical_list: D000928:Antidepressive Agents; D008567:Membranes, Artificial; C005062:acetylcellulose; D002482:Cellulose