MICA exon 5 microsatellite typing by DNA heteroduplex analysis: a new polymorphism in the transmembrane region.
MICA (MHC class I chain-related gene A) is localized 47 kb upstream from HLA-B on the short arm of chromosome 6. It has been postulated that MICA protein folds similarly to the class I chain and may have the capacity to bind short ligands. Short tandem repeats (STR) within the transmembrane (TM) region of this gene have been described and five alleles consisting of 4 to 9 GCT codons, each encoding an alanine residue have been defined. We have applied DNA heteroduplex analysis to type MICA trinucleotide repeats in order to develop a simple and reliable method for their identification. This approach allowed the characterization of all MICA alleles. Moreover, a new polymorphism within the TM region was identified.
Vitiani
L R
LR
Dept. of Cell Biology and Development, University of Rome La Sapienza, Roma, Italy.
Potolicchio
I
I
D'Amato
M
M
Baricordi
O R
OR
Sorrentino
R
R
D002460:Cell Line; D004247:DNA; D005091:Exons; D015395:Histocompatibility Antigens Class I / Q000235:genetics*; D006801:Humans; D018895:Microsatellite Repeats; D009692:Nucleic Acid Heteroduplexes; D011110:Polymorphism, Genetic
D015395:Histocompatibility Antigens Class I; C099187:MHC class I-related chain A; D009692:Nucleic Acid Heteroduplexes; D004247:DNA