Plasmids for the in vitro analysis of RNA polymerase II-dependent transcription based on a G-free template.
Described are a series of plasmids containing combinations of adenovirus-2 major late promoter elements, including consensus TATA box and initiator, upstream of G-free transcription cassettes of various lengths. These provide an assortment of tools for investigating both basal and regulated transcription mechanisms by in vitro transcription methods.
Wang
J C
JC
Department of Tumor Biology, The University of Texas M.D. Anderson Cancer Center, 1515 Holcombe Boulevard, Houston, TX 77030, USA.
Sawadogo
M
M
Van Dyke
M W
MW
D001483:Base Sequence; D005786:Gene Expression Regulation; D066298:In Vitro Techniques; D008969:Molecular Sequence Data; D010957:Plasmids; D011401:Promoter Regions, Genetic; D012319:RNA Polymerase II / Q000494:pharmacology*; D013698:Templates, Genetic; D014158:Transcription, Genetic
D012319:RNA Polymerase II