Murine mast cells and monocytes express distinctive sets of CD45 isoforms.
CD45 isoform expression patterns were examined in various mast and monocyte cell populations. The reverse transcription-polymerase chain reaction (RT/PCR) and Southern analysis showed these myeloid cells express characteristic sets of CD45 isoforms. Mast cells produce mRNA for two splice variants, one containing exons 5, 7 and 8 of the alternatively expressed exons (therefore lacking exons 4 and 6) and another containing variable exons 7 and 8. Monocytes express three prominent CD45 mRNA species, one which includes exons 5, 7 and 8, another with exons 7 and 8 and the third containing only exon 8 of the variable exons. These results show that there are clear differences within the myeloid lineage sub-populations with respect to CD45 exon usage which appear to delineate mast cell and monocyte specific patterns.
Virts
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E
Sidney Kimmel Cancer Center, San Diego, CA 92121, USA.
Barritt
D
D
Siden
E
E
Raschke
W C
WC
D017398:Alternative Splicing; D000818:Animals; D002460:Cell Line; D005091:Exons / Q000235:genetics; D017493:Leukocyte Common Antigens / Q000096:biosynthesis / Q000235:genetics* / Q000276:immunology*; D008407:Mast Cells / Q000276:immunology*; D051379:Mice; D009000:Monocytes / Q000276:immunology*; D012333:RNA, Messenger / Q000235:genetics
D012333:RNA, Messenger; D017493:Leukocyte Common Antigens