Low dose MK-801 protects against iron-induced oxidative changes in a rat model of focal epilepsy.
We have used chemiluminescence measurements to examine the relationship between free radical formation and excitotoxicity in a post-traumatic epilepsy model. For this purpose, seven days after injecting iron in rat brain cortices, we measured luminol- and lucigenin-enhanced chemiluminescence in different brain regions (ipsilateral cortex, contralateral cortex, hypothalamus and hippocampus). In all brain regions (except contralateral cortices) both luminol- and lucigenin-enhanced chemiluminescence were increased in iron-injected group compared to saline-injected control group. These increases returned to control values in iron-injected rats pretreated with MK-801. Our results suggest that both free radicals and excitatory amino acids play important roles in the development of post-traumatic epilepsy and that MK-801 has protective effects against iron-induced chemiluminescence formation.
Küçükkaya
B
B
Department of Biochemistry, Faculty of Medicine, Marmara University, Istanbul, Turkey.
Aker
R
R
Yüksel
M
M
Onat
F
F
Yalçin
A S
AS
D000704:Analysis of Variance; D000818:Animals; D004195:Disease Models, Animal; D016291:Dizocilpine Maleate / Q000494:pharmacology*; D004353:Drug Evaluation, Preclinical; D004569:Electroencephalography; D004828:Epilepsies, Partial / Q000188:drug therapy*; D018691:Excitatory Amino Acid Antagonists / Q000494:pharmacology*; D007501:Iron / Q000037:antagonists & inhibitors*; D008163:Luminescent Measurements; D018696:Neuroprotective Agents / Q000494:pharmacology*; D051381:Rats; D017207:Rats, Sprague-Dawley
D018691:Excitatory Amino Acid Antagonists; D018696:Neuroprotective Agents; D016291:Dizocilpine Maleate; D007501:Iron