Mechanism of translational coupling in the nifLA operon of Klebsiella pneumoniae.
The nifLA operon of Klebsiella pneumoniae encodes the sensor-activator pair involved in the regulation of other nif genes. Balanced synthesis of both proteins, which is required for correct regulation, is achieved by coupling translation of nifA to that of nifL. The mechanism of translational coupling at the nifLA operon was analysed using a specialized ribosome system, and the effect of substituting the natural Shine-Dalgarno of nifL or nifA for specialized Shine-Dalgarno sequences was determined. Our results indicate that translational coupling occurs in this operon by a reinitiation mechanism. Additionally, reinitiation at the nifA can happen even in the absence of good Shine-Dalgarno recognition by the reinitiating ribosome, although its efficiency is lower. The effect of a putative translational enhancer sequence (downstream box) on translational coupling efficiency was also determined. Mutations that reduce the homology of the putative downstream box to the consensus had only a minor effect on nifA translation by wild-type ribosomes. However, they had a significant effect on nifA translation by specialized ribosomes, suggesting that recognition of the downstream box may compensate inefficient ribosomal interactions with the Shine-Dalgarno sequence.
Govantes
F
F
Departamento de Genética, Facultad de Biología, Universidad de Sevilla, Ap. 1095, 41080-Sevilla, Spain.
Andújar
E
E
Santero
E
E
D001426:Bacterial Proteins / Q000235:genetics*; D001483:Base Sequence; D004247:DNA; D007544:Isopropyl Thiogalactoside; D007711:Klebsiella pneumoniae / Q000235:genetics*; D008969:Molecular Sequence Data; D016297:Mutagenesis, Site-Directed; D009690:Nucleic Acid Conformation; D009876:Operon; D014176:Protein Biosynthesis; D012270:Ribosomes; D014157:Transcription Factors / Q000235:genetics*
D001426:Bacterial Proteins; C061338:NifA protein, Bacteria; D014157:Transcription Factors; C084296:nifL protein, Bacteria; D007544:Isopropyl Thiogalactoside; D004247:DNA