title: Molecular cloning of a human cDNA for the 41-kDa phosphoribosylpyrophosphate synthetase-associated protein.
abstract: A human cDNA encoding 41-kDa phosphoribosylpyrophosphate (PRPP) synthetase (PRS)-associated protein (PAP41) was cloned from two expressed sequence tag (EST) clones having the nucleotide similarity of 61.5 and 70.0% to human PAP39 cDNA. The predicted open reading frame of 1107 base pairs (bp) has the nucleotide identity of 91.8% to rat PAP41 and encodes a protein of 369 amino acids with a calculated molecular weight (MW) of 40,925. The deduced amino acid sequence exhibits the 98.9% identity to rat PAP41 and 72.2, 50.6, and 50.0% identity with human PAP39, PRS I, and PRS II, respectively, but lacks the PRPP binding site. Southern blot analysis suggested that the PAP41 gene exists as a single copy in the human genome. The single PAP41 mRNA of about 2.1 kb was shown to be present in five human cell lines by Northern blot analysis.
authors.affiliation: Otsuka Department of Clinical and Molecular Nutrition, School of Medicine, University of Tokushima, Japan.
authors.forename: R
authors.initials: R
authors.lastname: Katashima
authors.forename: H
authors.initials: H
authors.lastname: Iwahana
authors.forename: M
authors.initials: M
authors.lastname: Fujimura
authors.forename: T
authors.initials: T
authors.lastname: Yamaoka
authors.forename: T
authors.initials: T
authors.lastname: Ishizuka
authors.forename: M
authors.initials: M
authors.lastname: Tatibana
authors.forename: M
authors.initials: M
authors.lastname: Itakura
mesh_terms: D000230:Adenocarcinoma; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D015139:Blotting, Southern; D018288:Carcinoma, Small Cell; D002460:Cell Line; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000302:isolation & purification*; D005347:Fibroblasts; D006367:HeLa Cells; D006801:Humans; D047908:Intracellular Signaling Peptides and Proteins; D008969:Molecular Sequence Data; D011506:Proteins / Q000235:genetics*; D051381:Rats; D012268:Ribose-Phosphate Pyrophosphokinase / Q000378:metabolism*; D017386:Sequence Homology, Amino Acid; D014407:Tumor Cells, Cultured
chemical_list: D018076:DNA, Complementary; D047908:Intracellular Signaling Peptides and Proteins; C103898:PAP41; D011506:Proteins; D012268:Ribose-Phosphate Pyrophosphokinase