title: Nasopharyngeal bacteria found on blood agar plates from healthy children in Greenland.
abstract: We have systematically studied the aerobic nasopharyngeal bacteria isolated from swabs by unselective subculturing on 5% horse blood agar and chocolate agar in 70 healthy children aged 0-1, 3-5 and 8 years in Nuuk and Sisimiut, Greenland. The purpose was to provide a basis for a better understanding of the infectious pathology and blind antibiotic treatment against potential pathogens thereby improving standard antimicrobial treatment of upper respiratory tract infections (URTI) and otitis media (OM) among children in Greenland. The study serves also as a baseline for future microbiological and immunological research projects. The children were clinically examined for any infectious diseases and a medical history was obtained which allowed for selection of children without a history of severe clinical infection. Nasopharyngeal swabs obtained via the oral route were instantly spread on 5% blood agar and chocolate agar culture plates and incubated aerobically. Subsequently, potentially pathogenic as well as non-pathogenic bacteria were identified by conventional methods. Healthy children in Greenland carry grossly the same aerobic bacterial flora as children in other parts of the world but potentially pathogenic bacteria were found in very high frequency (94%). Staphylococcus aureus, Streptococcus pneumoniae and Moraxella catarrhalis were found in higher frequencies in the youngest children. Haemophilus influenzae non-b was found in high frequencies in all age groups (67-76%). H. influenzae type b was carried by 11.4%. Group A streptococci were found more frequently in older children and in children from Sisimiut. Of M. catarrhalis strains 88% produced beta-lactamase. Neisseria meningitidis, Mycoplasma pneumoniae and chlamydiae were not detected at all. The high carrier frequency of potentially pathogenic bacteria in healthy children in Greenland may be related to the high frequency of URTI's and episodes of OM among children in Greenland.
authors.affiliation: Dept. of Otolaryngology, Head & Neck Surgery, Rigshospitalet, University of Copenhagen, Denmark. rh03259@rh.dk
authors.forename: P
authors.initials: P
authors.lastname: Homøe
authors.forename: J
authors.initials: J
authors.lastname: Prag
authors.forename: C B
authors.initials: CB
authors.lastname: Olsen
authors.forename: S
authors.initials: S
authors.lastname: Farholt
mesh_terms: D001420:Bacteria, Aerobic / Q000302:isolation & purification*; D001431:Bacteriological Techniques; D002648:Child; D002675:Child, Preschool; D003470:Culture Media; D005260:Female; D006116:Greenland; D006801:Humans; D007223:Infant; D008297:Male; D009305:Nasopharynx / Q000382:microbiology*; D010033:Otitis Media / Q000382:microbiology
chemical_list: D003470:Culture Media