Ultraviolet B irradiation modulates the immune system of fish (Rutilus rutilus, Cyprinidae). I. Phagocytes.
Roach (Rutilus rutilus) were irradiated with a single dose of ultraviolet B (UVB) radiation (0.4 J/cm2) in order to study the effects of UVB on the nonspecific immune defense mechanisms of fish. Neutrophils and macrophages were isolated from the head kidney of fish on days 1-14 postirradiation. Both random and directed migration of neutrophils, studied by migration under agarose assay, were suppressed on day 1 after UVB irradiation. The respiratory burst of phorbol 12-myristate 13-acetate-stimulated neutrophils and macrophages was also suppressed at days 1 and 2 after UVB irradiation. The suppression of migration and respiratory burst were restored or the responses were even enhanced later, but on the other hand spontaneous cytotoxicity of neutrophils toward 51chromium-labeled K562 target cells stayed suppressed throughout the 14 day follow-up. This study indicates that UVB radiation has the potential to suppress the functioning of phagocytes and to compromise the immune system of fish.
Salo
H M
HM
Department of Biological and Environmental Science, University of Jyväskylä, Finland. hamasa@jyu.fi
Aaltonen
T M
TM
Markkula
S E
SE
Jokinen
E I
EI
D000818:Animals; D002347:Carps / Q000276:immunology; D002634:Chemotaxis, Leukocyte / Q000528:radiation effects*; D005399:Fishes / Q000276:immunology*; D008221:Lymphoid Tissue / Q000528:radiation effects; D008264:Macrophages / Q000276:immunology / Q000528:radiation effects; D009504:Neutrophils / Q000502:physiology / Q000528:radiation effects*; D010586:Phagocytes / Q000187:drug effects / Q000276:immunology / Q000528:radiation effects*; D016897:Respiratory Burst / Q000187:drug effects / Q000528:radiation effects; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology; D014466:Ultraviolet Rays
D013755:Tetradecanoylphorbol Acetate