Nonradioactive sequence-tagged microsatellite site analyses: a method transferable to the tropics.
Utilization of existing isozyme analysis facilities to detect sequence-tagged microsatellite site (STMS) polymorphism or any simple sequence repeat (SSR) variation is described. Different parameters concerning the difficulties in transferring molecular techniques to less sophisticated laboratory infrastructures (i.e. tropical outstations) are discussed (e.g. reproducibility, efficacy, precision). Nonradioactive STMS analysis is bound to foster collaborative research between "biodiversity" and "biotechnology" centers.
Lagoda
P J
PJ
CIRAD BIOTROP, Montpellier, France. lagoda@cirad.fr
Dambier
D
D
Grapin
A
A
Baurens
F C
FC
Lanaud
C
C
Noyer
J L
JL
D001483:Base Sequence; D018744:DNA, Plant / Q000032:analysis / Q000235:genetics; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods*; D007553:Isotope Labeling; D018895:Microsatellite Repeats; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic; D016324:Sequence Tagged Sites
D018744:DNA, Plant