title: Developmental expression of alpha 9 acetylcholine receptor mRNA in the rat cochlea and vestibular inner ear.
abstract: Expression of alpha9 acetylcholine receptor (AChR) mRNA was studied by in situ hybridization in the rat adult and developing cochlea and vestibular inner ear. Alpha9 AChR mRNA was first observed in cochlear hair cells (HCs) at embryonic day 18 (E18), increased markedly after birth, stayed high until postnatal day 10 (P10), and decreased to substantially lower adult levels by P14. High levels of alpha9 AChR mRNA expression were also noted in the developing nonneuronal structures of the inner sulcus, chondrocytes, and/or osteoblasts in the cochlear capsule and interscalar laminae. Both developing and adult bone marrow cells also expressed intense alpha9 AChR mRNA. In the vestibular system, alpha9 AChR mRNA was first observed in HCs at E16 in all sensory epithelia, increased to its highest levels by P0-P4, then decreased slightly to reach adult levels by P10. The results are consistent with the alpha9 AChR subserving efferent neurotransmission to both cochlear and vestibular HCs. The observation of alpha9 AChR mRNA in cochlear HCs 2 weeks prior to functional onset in the cochlea further suggests that expression of this gene is not related to HC activity. The observation of substantial nonneuronal expression of alpha9 AChR mRNA suggests that this receptor also has functions separate from its role in neurotransmission.
authors.affiliation: Department of Surgery/Otolaryngology, UCSD School of Medicine and VA Medical Center, La Jolla, California 92093-0666, USA.
authors.forename: L
authors.initials: L
authors.lastname: Luo
authors.forename: T
authors.initials: T
authors.lastname: Bennett
authors.forename: H H
authors.initials: HH
authors.lastname: Jung
authors.forename: A F
authors.initials: AF
authors.lastname: Ryan
mesh_terms: D000818:Animals; D003001:Cloning, Molecular; D003051:Cochlea / Q000166:cytology / Q000378:metabolism*; D018507:Gene Expression Regulation, Developmental; D006168:Guinea Pigs; D009475:Neurons, Afferent / Q000378:metabolism; D009925:Organ of Corti / Q000378:metabolism; D012333:RNA, Messenger / Q000096:biosynthesis; D051381:Rats; D017207:Rats, Sprague-Dawley; D011950:Receptors, Cholinergic / Q000096:biosynthesis / Q000235:genetics*; D014722:Vestibule, Labyrinth / Q000378:metabolism*
chemical_list: D012333:RNA, Messenger; D011950:Receptors, Cholinergic