Phorbol ester induces differentiation of a human prostatic cancer cell line TSU-Pr1 into cells with characteristics of microglia.
The effects of various reagents on the induction of differentiation of the human prostatic cancer cell line, TSU-Pr1, were examined. Among these agents, the phorbol ester, TPA, almost completely suppressed cell proliferation at the concentration of 10(-8) M, and induced remarkable morphologic changes yielding cells with the microglial feature of an ameboid and/or ramified shape. More than 90% of the cells underwent the induction of morphologic changes by day 7 after treatment with 10(-8) M TPA. The expression of reliable microglial markers, alpha-naphthyl acetate esterase activity and CD11b, was observed in the differentiated cells. The data presented here suggest that TPA induces differentiation of a human prostate cancer cell line into cells with the characteristics of microglia.
Itayasu
T
T
Department of Hygiene-Chemistry, Faculty of Pharmaceutical Sciences, Science University of Tokyo, Japan. takeda@ps.kagu.sut.ac.jp
Shimizu
T
T
Iizumi
T
T
Oshio
S
S
Umeda
T
T
Takeda
K
K
D002454:Cell Differentiation / Q000187:drug effects; D002455:Cell Division / Q000187:drug effects; D006801:Humans; D008207:Lymphatic Metastasis; D016177:Macrophage-1 Antigen / Q000378:metabolism; D008297:Male; D017628:Microglia / Q000201:enzymology / Q000473:pathology*; D009283:Naphthol AS D Esterase / Q000378:metabolism; D011471:Prostatic Neoplasms / Q000473:pathology*; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology*; D014407:Tumor Cells, Cultured / Q000166:cytology
D016177:Macrophage-1 Antigen; D009283:Naphthol AS D Esterase; D013755:Tetradecanoylphorbol Acetate