Comparison between Sulphorhodamine-B dye staining and 51Cr-release method in cytotoxicity assay of endodontic sealers.
The aim of this study was to evaluate Sulphorhodamine-B (SRB) staining against 51Cr-release in cytotoxicity tests of six endodontic sealers, namely, MU sealer (Mahidol University) ROCANAL 2, ROCANAL 3, Apexit, Endomethasone, and AH-26. Monolayers (5 x 10(5) cells/ml) of the mouse cell line Mu-mu-1 were used as test cells. Following incubation at 37 degrees C in 5% CO2 for 24 h in the presence of each sealer, cells were stained with 0.4% SRB and the absorbance at 540 nm determined as measure of cell viability. For 51Cr-release assay, cells were labelled with 51Cr before testing with sealers, and radioactivity in the supernatant was measured in a liquid scintillation counter. Both techniques indicated that Apexit was the least toxic sealer. In view of the ease of conducting SRB staining for tests of cell viability, this may be the method of choice over 51Cr-release assay in the evaluation of endodontic sealer cytotoxicity.
Vajrabhaya
L
L
Department of Operative Dentistry, Faculty of Dentistry, Mahidol University, Bangkok, Thailand.
Sithisarn
P
P
Wilairat
P
P
Leelaphiwat
S
S
D000704:Analysis of Variance; D000818:Animals; D002860:Chromium Radioisotopes; D005347:Fibroblasts / Q000187:drug effects*; D051379:Mice; D012235:Rhodamines; D012387:Root Canal Filling Materials / Q000633:toxicity*; D018675:Toxicity Tests
D002860:Chromium Radioisotopes; D012235:Rhodamines; D012387:Root Canal Filling Materials; C022027:lissamine rhodamine B