title: The effect of azadirachtin on fresh isolates of Trypanosoma cruzi in different species of triatomines.
abstract: The effect of azadirachtin was investigated using three different fresh isolates of Trypanosoma cruzi and five different triatomine species which were infected as third-instar larvae. The two T. cruzi strains which originated from sylvatic Triatoma vitticeps showed a high prevalence after the molt to the fifth instar in Panstrongylus megistus and Rhodnius neglectus and a low prevalence in Triatoma infestans and Rhodnius robustus. The third T. cruzi strain originating from a patient in Piaui showed a high prevalence in P. megistus, R. neglectus and T. infestans and a low prevalence in Triatoma sordida. Feeding the infected fifth instars with azadirachtin-supplemented blood (1 microg/ml) resulted 20 days later in some parasite/vector combinations in an increase, in others a decrease or an unchanged number of T. cruzi in comparison to bugs fed with unsupplemented blood.
authors.affiliation: Department of Special Zoology, Ruhr University, Bochum, Germany. Guenter.A.Schaub@rz.ruhr-uni-bochum.de
authors.forename: A H
authors.initials: AH
authors.lastname: Kollien
authors.forename: T C
authors.initials: TC
authors.lastname: Gonçalves
authors.forename: P
authors.initials: P
authors.lastname: De Azambuja
authors.forename: E S
authors.initials: ES
authors.lastname: Garcia
authors.forename: G A
authors.initials: GA
authors.lastname: Schaub
mesh_terms: D000818:Animals; D006790:Host-Parasite Interactions; D006801:Humans; D007303:Insect Vectors / Q000469:parasitology*; D036701:Limonins; D051379:Mice; D013045:Species Specificity; D014224:Triatoma / Q000469:parasitology*; D014315:Triterpenes / Q000494:pharmacology*; D014344:Trypanocidal Agents / Q000494:pharmacology; D014349:Trypanosoma cruzi / Q000187:drug effects* / Q000254:growth & development
chemical_list: D036701:Limonins; D014315:Triterpenes; D014344:Trypanocidal Agents; C010329:azadirachtin