title: Bordetella parapertussis infection in children: epidemiology, clinical symptoms, and molecular characteristics of isolates.
abstract: The clinical trial conducted in Italy to evaluate the efficacy of acellular pertussis vaccines provided an opportunity to estimate the frequency of clinical infections with Bordetella parapertussis and to compare the clinical characteristics of children suffering from Bordetella pertussis illness with those of children with B. parapertussis illness. This study dealt with 76 B. parapertussis infections diagnosed from a population of 15,601 children participating in the follow-up of suspected cases of pertussis. An overall incidence of 2.1 cases of laboratory-confirmed parapertussis per 1,000 person-years was observed. Children affected by B. parapertussis infections showed a less severe clinical picture both in the duration of symptoms and in the percentage of patients affected, even when compared with vaccinated children with pertussis. To characterize the isolated strains, we performed assays for susceptibility to erythromycin and sulfamethoxazole-trimethoprim, and we examined the genomic DNAs by pulsed-field gel electrophoresis. The results showed a high degree of genetic stability among B. parapertussis strains regardless of time of collection and geographical distribution.
authors.affiliation: Department of Bacteriology and Medical Mycology, Istituto Superiore di Sanità, Rome, Italy. pmastran@net.iss.it
authors.forename: P
authors.initials: P
authors.lastname: Mastrantonio
authors.forename: P
authors.initials: P
authors.lastname: Stefanelli
authors.forename: M
authors.initials: M
authors.lastname: Giuliano
authors.forename: Y
authors.initials: Y
authors.lastname: Herrera Rojas
authors.forename: M
authors.initials: M
authors.lastname: Ciofi degli Atti
authors.forename: A
authors.initials: A
authors.lastname: Anemona
authors.forename: A E
authors.initials: AE
authors.lastname: Tozzi
mesh_terms: D001884:Bordetella / Q000187:drug effects / Q000235:genetics*; D016521:Electrophoresis, Gel, Pulsed-Field; D006801:Humans; D015994:Incidence; D007223:Infant; D008826:Microbial Sensitivity Tests; D014917:Whooping Cough / Q000453:epidemiology* / Q000517:prevention & control