Differentiation of Cryptosporidium parvum isolates by a simplified randomly amplified polymorphic DNA technique.
Genomic DNA was isolated from Cryptosporidium parvum oocysts by a specific immunomagnetic separation-in vitro excystation procedure and subjected to randomly amplified polymorphic DNA analysis using sequence-independent primers. An estuary C. parvum isolate was easily differentiated from several bovine isolates, while five bovine isolates of the same origin were indistinguishable from each other.
Deng
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Department of Population Health and Reproduction, School of Veterinary Medicine, University of California-Davis 95616, USA.
Cliver
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DO
D000818:Animals; D002417:Cattle; D016785:Cryptosporidium parvum / Q000235:genetics*; D016054:DNA, Protozoan / Q000032:analysis*; D016133:Polymerase Chain Reaction; D019105:Random Amplified Polymorphic DNA Technique
D016054:DNA, Protozoan