Syntaxin 11: a member of the syntaxin family without a carboxyl terminal transmembrane domain.
We have cloned a novel syntaxin-like molecule, designated human syntaxin 11 (hsyn11). The open reading frame encodes a polypeptide of 287 amino acids with potential coiled-coil domains. hsyn11 has extensive homology to members of the syntaxin family, particularly syntaxin 1 and syntaxin 2. Unlike other members of the syntaxin family, however, hsyn11 has a short cysteinerich carboxyl-terminal tail but not a typical hydrophobic domain which may serve as a membrane anchor. Northern blot analysis revealed two transcripts of approximately 0.8 kb and approximately 1.7 kb in length that are particularly abundant in heart and placenta, although lower levels were also detectable in other tissues except in the brain. Consistent with the lack of a distinct membrane anchorage sequence in hsyn11, indirect immunofluorescence microscopy of transiently expressed N-terminally myc-tagged hsyn11 revealed a diffuse, cytoplasmic labeling.
Tang
B L
BL
Membrane Biology Laboratory, Institute of Molecular and Cell Biology, Singapore, Republic of Singapore.
Low
D Y
DY
Hong
W
W
D000595:Amino Acid Sequence; D001483:Base Sequence; D002460:Cell Line; D003001:Cloning, Molecular; D003593:Cytoplasm / Q000737:chemistry; D006801:Humans; D008565:Membrane Proteins / Q000737:chemistry*; D008856:Microscopy, Fluorescence; D008969:Molecular Sequence Data; D050765:Qa-SNARE Proteins; D012333:RNA, Messenger / Q000378:metabolism; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D050827:Syntaxin 1; D014162:Transfection / Q000235:genetics
D008565:Membrane Proteins; D050765:Qa-SNARE Proteins; D012333:RNA, Messenger; C494212:STX1A protein, human; D050827:Syntaxin 1