title: Time course of changes in the intestinal permeability of food-sensitized rats after oral allergen challenge.
abstract: Rats were sensitized with ovalbumin (OVA) with a molecular weight of 45 kd, challenged with OVA orally, followed by orally administered beta-lactoglobulin (BLG) as an intestinal permeability marker. BLG is a macro-molecular protein with a molecular weight of 18 kd. Blood BLG concentrations were measured (by ELISA) serially over 4 hours following BLG administration, which in turn was given 1 hour after OVA challenges. The maximum BLG concentration was at 2 hours. BLG was then administered orally 1, 3, 6, 12 and 24 hours after oral OVA challenge, and the serum BLG concentration at 2 hours after BLG administration was compared among the five groups. BLG appeared in the circulation of the animals 1, 6 and 24 hours after allergen challenge, but not after 3 and 12 hours. The serum BLG concentration was not significantly different at 1, 6 and 24 hours. Histopathological examinations of the intestines showed mast cell infiltration of the intestinal mucosa at 1 hour, remarkable edema of villi at 3 hours, eosinophil infiltration at 6 hours, an increase of goblet cells at 12 hours and villous atrophy and lymphocyte infiltration at 24 hours. The appearance in the serum of three BLG peaks of comparable heights suggested that the intestinal absorption of BLG may be related to a late and delayed phase as well as the immediate IgE-dependent phase response.
authors.affiliation: Department of Paediatrics, Showa University School of Medicine, Tokyo, Japan.
authors.forename: Y
authors.initials: Y
authors.lastname: Sakamoto
authors.forename: T
authors.initials: T
authors.lastname: Ohtsuka
authors.forename: H
authors.initials: H
authors.lastname: Yoshida
authors.forename: K
authors.initials: K
authors.lastname: Ohto
authors.forename: M
authors.initials: M
authors.lastname: Onobori
authors.forename: T
authors.initials: T
authors.lastname: Matsumoto
authors.forename: Y
authors.initials: Y
authors.lastname: Iikura
authors.forename: T
authors.initials: T
authors.lastname: Morohoshi
mesh_terms: D000284:Administration, Oral; D000818:Animals; D005512:Food Hypersensitivity / Q000276:immunology / Q000473:pathology / Q000503:physiopathology*; D007073:Immunoglobulin E / Q000097:blood; D007408:Intestinal Absorption; D007413:Intestinal Mucosa / Q000378:metabolism* / Q000473:pathology; D007422:Intestines / Q000276:immunology / Q000473:pathology; D007782:Lactoglobulins / Q000008:administration & dosage / Q000097:blood / Q000378:metabolism*; D008297:Male; D010047:Ovalbumin / Q000008:administration & dosage / Q000009:adverse effects* / Q000276:immunology; D010323:Passive Cutaneous Anaphylaxis; D010539:Permeability; D051381:Rats; D011914:Rats, Inbred BN; D013997:Time Factors
chemical_list: D007782:Lactoglobulins; D007073:Immunoglobulin E; D010047:Ovalbumin