Sequencing and high level expression in Escherichia coli of the tropomyosin allergen (Der p 10) from Dermatophagoides pteronyssinus.
The cDNA encoding an allergen from the dust mite Dermatophagoides pteronyssinus has been cloned and sequenced. The allergen (Der p 10) is a tropomyosin that shared more than 65% identical residues with other invertebrate tropomyosins. The final recovery of recombinant Der p 10 from the culture media after a single purification step was as much as 26 mg/l. The recombinant allergen is reactive to shrimp antitropomyosin IgG antibodies and has a 5.6% frequency of IgE reactivity in sera from mite-allergic patients.
Asturias
J A
JA
IFIDESA-ARISTEGUI, Research and Development Department, Bilbao, Spain. im000001@sarenet.es
Arilla
M C
MC
Gómez-Bayón
N
N
Martínez
A
A
Martínez
J
J
Palacios
R
R
D000485:Allergens / Q000235:genetics* / Q000276:immunology* / Q000378:metabolism; D000595:Amino Acid Sequence; D000818:Animals; D039741:Antigens, Dermatophagoides; D052179:Antigens, Plant; D060829:Arthropod Proteins; D001483:Base Sequence; D015153:Blotting, Western; D004926:Escherichia coli / Q000235:genetics; D006801:Humans; D006967:Hypersensitivity / Q000276:immunology; D007106:Immune Sera; D007073:Immunoglobulin E / Q000378:metabolism; D008925:Mites / Q000235:genetics*; D008969:Molecular Sequence Data; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism; D017386:Sequence Homology, Amino Acid; D014335:Tropomyosin / Q000235:genetics* / Q000276:immunology / Q000378:metabolism
D000485:Allergens; D039741:Antigens, Dermatophagoides; D052179:Antigens, Plant; D060829:Arthropod Proteins; D007106:Immune Sera; D011994:Recombinant Proteins; D014335:Tropomyosin; C111884:der p 10 protein, Dermatophagoides pteronyssinus; D007073:Immunoglobulin E