Cytogenetic chromosomal preparations using 2-methoxyestradiol.
The antimitotic drug 2-methoxyestradiol is an end metabolite of catechol estrogens. In vivo, it arrests cells in mitosis by interfering with the dynamics of the mitotic spindle without disrupting tubulin formation. It has a mitotic index similar to that of Colcemid in different cell lines. Here we report that 2-methoxyestradiol can be used for making cytogenetic preparations of comparable quality to that of colcemid. In addition, 2-methoxyestradiol is devoid of the toxicity associated with Colcemid, which may make 2-methoxyestradiol useful in slowly growing samples often found in primary solid tumor cultures where a sufficient number of mitotic cells is difficult to obtain.
Attalla
H
H
Department of Clinical Chemistry, University of Helsinki, Finland.
Knuutila
S
S
Mäkelä
T P
TP
Andersson
L C
LC
Adlercreutz
H
H
D000077584:2-Methoxyestradiol; D001854:Bone Marrow Cells; D002478:Cells, Cultured; D002871:Chromosome Banding / Q000379:methods*; D003703:Demecolcine; D004958:Estradiol / Q000031:analogs & derivatives*; D005347:Fibroblasts; D006801:Humans; D007621:Karyotyping / Q000379:methods*; D007962:Leukocytes; D008938:Mitosis
D004958:Estradiol; D000077584:2-Methoxyestradiol; D003703:Demecolcine