Natural and therapy-induced anti-GM-CSF and anti-G-CSF antibodies in human serum.
Serum samples were obtained from patients with lymphoid and plasma cell malignancies who received after chemotherapy human recombinant GM-CSF or G-CSF. Sera from some patients revealed the presence of anti-cytokine antibodies, particularly after repetitive cytokine injections. Antibody Fab binding in a saturable manner by ELISA and Western immuno-blotting confirmed antibody specificity. Anti-cytokine antibodies were detected before the exogenous cytokine injections in some patients, but increasing antibody levels were found after one or subsequent treatments. Low levels of anti-GM-CSF and anti-G-CSF antibodies were also detected in a relatively large proportion (about 10-30%) of normal sera from different adult healthy volunteers who had never been treated before with exologous cytokines as well as from cord blood. EBV-immortalized cord blood derived B-cell cultures were also found to produce anti GM-CSF and/or anti-G-CSF antibodies with high frequency.
Revoltella
R P
RP
Institute of Mutagenesis and Differentiation, C.N.R., Pisa, Italy. r.revoltella@imd.pi.cnr.it
Laricchia-Robbio
L
L
Moscato
S
S
Genua
A
A
Liberati
A M
AM
D000328:Adult; D000906:Antibodies / Q000097:blood* / Q000276:immunology; D000918:Antibody Specificity; D015153:Blotting, Western; D016179:Granulocyte Colony-Stimulating Factor / Q000276:immunology* / Q000627:therapeutic use; D016178:Granulocyte-Macrophage Colony-Stimulating Factor / Q000276:immunology* / Q000627:therapeutic use; D019337:Hematologic Neoplasms / Q000097:blood / Q000188:drug therapy / Q000276:immunology*; D006801:Humans
D000906:Antibodies; D016179:Granulocyte Colony-Stimulating Factor; D016178:Granulocyte-Macrophage Colony-Stimulating Factor