title: Evaluation of the Sysmex UF-100 automated urinalysis analyzer.
abstract: Urinalysis is a high-volume procedure that currently requires significant labor to examine microscopic sediment. We evaluated the Sysmex UF-100 automated urinalysis analyzer for performing this task. Instrument accuracy was assessed by comparing continuous counts of microscopic elements from the UF-100 with ranges of cells (per low-power field or high-power field) from manual microscopy performed on centrifuged urines. Counts showed good agreement between methods (gamma statistic: 0.880-0.970) for all microscopic elements in 252 urine samples. Within-run imprecision of cell counts expressed as CV (mean cell count/microL) was for erythrocytes (RBC) 31% (5), 18% (50), 2.4% (800); for leukocytes (WBC) 14% (10), 11% (100), 8.5% (400); for squamous epithelial cells (SEC) 18% (5), 12% (30), 7.0% (100); for casts 45% (1), 17% (4); for bacteria 2-12% (entire range of 40-2500). Between-run imprecision on quality-control cell suspensions expressed as CV (mean cell count/microL) was for RBC 6.1% (50), 2.7% (256); for WBC 26.9% (54), 4.9% (228). Cells counted on dilution were 99.1% of expected for RBC, 102.0% for WBC, and 121.8% for bacteria. Carryover was <0.04% for RBC, <0.03% for WBC, <0.14% for SEC, <0.29% for bacteria. We conclude that the UF-100 can automatically perform reliable quantitative microscopic urinalysis in batches without operator interaction.
authors.affiliation: Department of Pathology, Virginia Commonwealth University/Medical College of Virginia, Richmond 23298-0250, USA. JBENEZRA@HSC.VCU.EDU
authors.forename: J
authors.initials: J
authors.lastname: Ben-Ezra
authors.forename: L
authors.initials: L
authors.lastname: Bork
authors.forename: R A
authors.initials: RA
authors.lastname: McPherson
mesh_terms: D001322:Autoanalysis / Q000295:instrumentation; D001419:Bacteria / Q000166:cytology; D002452:Cell Count / Q000295:instrumentation; D004847:Epithelial Cells; D004912:Erythrocytes; D006801:Humans; D007962:Leukocytes; D016482:Urinalysis / Q000295:instrumentation*; D014556:Urine / Q000166:cytology / Q000382:microbiology