Quantification of Eu3+ in quantum-dye-labeled materials by ashing and dissociation enhancement.
Quantum Dye (QD) is an extremely stable macrocyclic chelate of Eu3+, measurable with a time-resolved fluorometer even without the aid of enhancement solution, albeit at a low sensitivity. However, QD is not easily quantified by the popular dissociation-enhancement methodology (I. Hemmilä et al., 1984, Anal. Biochem. 137, 335-343), because of difficulty in dissociating Eu3+ completely from QD. We found that Eu3+ in QD can be accurately measured by simple ashing followed by the dissociation-enhancement methodology. Validity of the method was tested by determining Eu(NO3)3 with or without ashing and analyzing Eu3+ in other chelates known to be amenable to accurate measurement by the dissociation enhancement.
Saito
K
K
Department of Biology, Johns Hopkins University, Baltimore, Maryland 21218, USA.
Lee
R T
RT
Lee
Y C
YC
D002412:Cations; D002614:Chelating Agents / Q000737:chemistry; D004396:Coloring Agents; D005063:Europium / Q000032:analysis*; D005453:Fluorescence
D002412:Cations; D002614:Chelating Agents; D004396:Coloring Agents; D005063:Europium