title: Physical map of the D6S149-D6S193 region on chromosome 6Q27 and its involvement in benign surface epithelial ovarian tumours.
abstract: A detailed long range restriction map of the region defined by markers D6S149 and D6S193 on chromosome 6q27 has been constructed. This was achieved by YAC cloning and contig assembling of the same region. Seven YAC clones were found to span the almost 1000 Kb region flanked by the two markers which on the genetic map resulted to be 1.9 cM apart. With some of the characterized YAC clones we undertook a molecular cytogenetic analysis of 20 benign ovarian tumors. The rationale for this was the recent mapping to a region of chromosome 6q27, flanked by markers D6281 and D6S133, of a locus for the SV40-mediated immortalization of human cells (SEN6 gene). Noteworthy we found that the the D6S149-D6S193 region (comprised in the larger D6S281-D6S133 physical interval) was altered in all samples analysed adding support to the occurrence of a immortalization step in this type of tumors.
authors.affiliation: Dipartimento di Patologia, Universita' degli Studi di Pavia (sede Varese), Ospedale di Circolo Varese, Italy.
authors.forename: M G
authors.initials: MG
authors.lastname: Tibiletti
authors.forename: M
authors.initials: M
authors.lastname: Trubia
authors.forename: E
authors.initials: E
authors.lastname: Ponti
authors.forename: L
authors.initials: L
authors.lastname: Sessa
authors.forename: F
authors.initials: F
authors.lastname: Acquati
authors.forename: D
authors.initials: D
authors.lastname: Furlan
authors.forename: B
authors.initials: B
authors.lastname: Bernasconi
authors.forename: M
authors.initials: M
authors.lastname: Fichera
authors.forename: A
authors.initials: A
authors.lastname: Mihalich
authors.forename: A
authors.initials: A
authors.lastname: Ziegler
authors.forename: A
authors.initials: A
authors.lastname: Volz
authors.forename: C
authors.initials: C
authors.lastname: Facco
authors.forename: C
authors.initials: C
authors.lastname: Riva
authors.forename: L
authors.initials: L
authors.lastname: Cremonesi
authors.forename: M
authors.initials: M
authors.lastname: Ferrari
authors.forename: R
authors.initials: R
authors.lastname: Taramelli
mesh_terms: D002872:Chromosome Deletion; D002896:Chromosomes, Human, Pair 6 / Q000235:genetics*; D005260:Female; D005819:Genetic Markers; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D009375:Neoplasms, Glandular and Epithelial / Q000235:genetics*; D010051:Ovarian Neoplasms / Q000235:genetics*; D015183:Restriction Mapping
chemical_list: D005819:Genetic Markers