title: Efficacies of alkaline protease, elastase and exotoxin A toxoid vaccines against gut-derived Pseudomonas aeruginosa sepsis in mice.
abstract: The protective efficacies of vaccines prepared from Pseudomonas aeruginosa alkaline protease, elastase and exotoxin A toxoids against gut-derived P. aeruginosa sepsis in mice were evaluated. Specific pathogen-free mice given P. aeruginosa strain D4 orally followed by cyclophosphamide (to promote translocation across the gut wall) died of bacteraemia. Mice immunised with one of the three individual toxoid vaccines were not significantly protected when compared to control mice immunised with bovine serum albumin. Combined immunisation with alkaline protease and elastase toxoids likewise showed no significant protective activity. However, combined immunisation with alkaline protease and exotoxin A toxoids significantly increased the survival rate, which reached 60% (compared with a 7.1% survival rate in the control group). These results show that alkaline protease and exotoxin A play important roles as pathogenic factors in gut-derived sepsis and that a combination of the two exoenzyme toxoids represents a logical candidate for vaccination against P. aeruginosa sepsis.
authors.affiliation: Department of Microbiology, Toho University School of Medicine, Tokyo, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Matsumoto
authors.forename: K
authors.initials: K
authors.lastname: Tateda
authors.forename: N
authors.initials: N
authors.lastname: Furuya
authors.forename: S
authors.initials: S
authors.lastname: Miyazaki
authors.forename: A
authors.initials: A
authors.lastname: Ohno
authors.forename: Y
authors.initials: Y
authors.lastname: Ishii
authors.forename: Y
authors.initials: Y
authors.lastname: Hirakata
authors.forename: K
authors.initials: K
authors.lastname: Yamaguchi
mesh_terms: D036002:ADP Ribose Transferases; D000818:Animals; D000907:Antibodies, Bacterial / Q000096:biosynthesis / Q000097:blood; D001427:Bacterial Toxins; D001428:Bacterial Vaccines; D005098:Exotoxins / Q000276:immunology*; D007114:Immunization; D007074:Immunoglobulin G / Q000096:biosynthesis / Q000097:blood; D008297:Male; D051379:Mice; D010196:Pancreatic Elastase / Q000276:immunology*; D011552:Pseudomonas Infections / Q000401:mortality / Q000517:prevention & control*; D011550:Pseudomonas aeruginosa / Q000201:enzymology / Q000276:immunology*; D012697:Serine Endopeptidases / Q000276:immunology*; D015996:Survival Rate; D014121:Toxoids / Q000276:immunology; D037521:Virulence Factors; D000097668:Pseudomonas aeruginosa Exotoxin A
chemical_list: D036002:ADP Ribose Transferases; D000907:Antibodies, Bacterial; D001427:Bacterial Toxins; D001428:Bacterial Vaccines; D005098:Exotoxins; D007074:Immunoglobulin G; D010196:Pancreatic Elastase; D012697:Serine Endopeptidases; D014121:Toxoids; D037521:Virulence Factors; D000097668:Pseudomonas aeruginosa Exotoxin A; C032923:microbial serine proteinases