[Analysis of B-epitopes formed on proteins after treatment with glutaraldehyde].
Structural analysis of the B-epitopes formed on protein macromolecules by glutaraldehyde treatment with subsequent block of free active groups by glycine was performed. Two types of antigenic determinants recognized by different antibody populations were found by protein chemistry and immunochemistry techniques. An efficient method of blocking the antibodies to the epitopes formed on proteins by glutaraldehyde was developed.
Kavun
E M
EM
Palladin Institute of Biochemistry, National Academy of Sciences of Ukraine, Kiev, Ukraine.
Kolibo
D V
DV
Borisova
E G
EG
D000818:Animals; D000906:Antibodies / Q000276:immunology*; D000936:Antigen-Antibody Complex / Q000276:immunology; D002846:Chromatography, Affinity; D003432:Cross-Linking Reagents / Q000737:chemistry*; D004797:Enzyme-Linked Immunosorbent Assay; D000939:Epitopes / Q000737:chemistry*; D005976:Glutaral / Q000737:chemistry / Q000276:immunology*; D010455:Peptides / Q000737:chemistry / Q000276:immunology*; D011817:Rabbits
D000906:Antibodies; D000936:Antigen-Antibody Complex; D003432:Cross-Linking Reagents; D000939:Epitopes; D010455:Peptides; D005976:Glutaral