Activation of SV40 DNA replication in vivo by amplification-promoting sequences of the mouse ribosomal gene cluster.
Mouse genomic DNA sequences from the region upstream of the ribosomal genes reported to contain amplification-promoting activity (APS1 and APS2) were tested for their ability to support SV40 replication in vivo. Both APSs can functionally replace the auxiliary sequence 2 on the late side of the SV40 origin, which facilitates initiation of replication. This stimulatory activity on the SV40 origin was orientation independent and was exerted over a distance of at least 0.9 kb. However, the APS2 element can not substitute for the auxiliary sequence 1 on the early side of the origin. Although both APS elements contain various prospective transcription factor binding sites, they do not trans-activate transcription from a minimal promoter. In this respect they differ from the enhancer element of the auxiliary sequence 2 of the SV40 origin. These results suggest that the rDNA sequences tested contain genetic information facilitating replication initiation on a viral core origin.
Staib
C
C
Institute of Biochemistry, University of Würzburg, Germany. staib@biozentrum.uni-wuerzburg.de
Wegner
M
M
Grummt
F
F
D000818:Animals; D001665:Binding Sites; D019556:COS Cells; D002460:Cell Line; D004261:DNA Replication / Q000235:genetics* / Q000502:physiology; D004275:DNA, Ribosomal / Q000235:genetics*; D004279:DNA, Viral / Q000096:biosynthesis* / Q000235:genetics*; D005784:Gene Amplification; D051379:Mice; D005810:Multigene Family; D011401:Promoter Regions, Genetic; D018741:Replication Origin; D013539:Simian virus 40 / Q000235:genetics* / Q000502:physiology; D014157:Transcription Factors / Q000378:metabolism
D004275:DNA, Ribosomal; D004279:DNA, Viral; D014157:Transcription Factors