title: Substitution of Asn for Ser543 in the large fragment of Taq DNA polymerase increases the efficiency of synthesis of long DNA molecules.
abstract: Substitution of Asn for Ser543 in the large fragment of Taq DNA polymerase (Klentaq) increases several times the efficiency of synthesis of long (over 2 kbp) DNA molecules. The difference in the DNA synthesis efficiencies by the mutant and native enzymes increased with the increase in the DNA fragment length.
authors.affiliation: Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Moscow.
authors.forename: K B
authors.initials: KB
authors.lastname: Ignatov
authors.forename: A I
authors.initials: AI
authors.lastname: Miroshnikov
authors.forename: V M
authors.initials: VM
authors.lastname: Kramarov
mesh_terms: D001216:Asparagine / Q000235:genetics / Q000378:metabolism*; D010582:Bacteriophage lambda / Q000235:genetics; D004279:DNA, Viral / Q000096:biosynthesis*; D016297:Mutagenesis, Site-Directed; D012694:Serine / Q000235:genetics / Q000378:metabolism*; D019914:Taq Polymerase / Q000235:genetics / Q000378:metabolism*
chemical_list: D004279:DNA, Viral; D012694:Serine; D001216:Asparagine; D019914:Taq Polymerase