The role of inverted repeat (IR) sequence of the virE gene expression in Agrobacterium tumefaciens pTiA6.
Agrobacterium tumefaciens pTiA6 virE promoter has a vir box, an inverted repeat (IR) sequence, a putative -35 region and a consensus -10 region. To study how the IR sequence of the virE promoter plays a role in virE gene expression, various mutants were constructed by base substitution and deletion in the virE promoter region. Substitution of the 3'-end region of the IR sequence, 5'-TCCGTTTCAA-3' to 5'-GCGGCCGCTC-3' displayed 2.6% of the native virE promoter activity. A deletion mutant of 5'-CGTTTCAA-3' on the 3'-end region of the IR sequence expressed 6% of the native virE promoter activity. These mutational analyses demonstrated that the IR sequence of the virE promoter plays a role as a cis-acting element in virE expression.
Jeon
G A
GA
Department of Biology, Korea University, Seoul, Korea.
Eum
J
J
Sim
W S
WS
D016960:Agrobacterium tumefaciens / Q000235:genetics*; D001426:Bacterial Proteins / Q000235:genetics*; D001483:Base Sequence; D015964:Gene Expression Regulation, Bacterial / Q000502:physiology*; D005798:Genes, Bacterial / Q000502:physiology*; D008969:Molecular Sequence Data; D016297:Mutagenesis, Site-Directed; D011401:Promoter Regions, Genetic / Q000235:genetics; D012091:Repetitive Sequences, Nucleic Acid / Q000502:physiology*; D037521:Virulence Factors; D001616:beta-Galactosidase / Q000378:metabolism
D001426:Bacterial Proteins; D037521:Virulence Factors; D001616:beta-Galactosidase