Effect of a novel recombinant bovine interferon and trophoblast secretory products on protein metabolism by endometrial explants from cattle and sheep.
The effect of a novel recombinant bovine interferon on the rate of protein synthesis, as measured by the rate of leucine incorporation, in endometrial explants from cows and ewes was investigated and compared with the effect of trophoblast secretory proteins and of a recombinant bovine interferon-alpha1. Exposure of cultured bovine endometrial tissue to the novel recombinant bovine interferon mimicked the effect of pregnancy in reducing the rate of protein synthesis in both caruncular and intercaruncular endometrium. This effect was similar to that seen with naturally produced trophoblast secretory proteins and it was more pronounced than that seen when endometrial tissue was treated with a related recombinant bovine interferon-alpha1. Ovine endometrial cultures were unaffected by exposure to this novel recombinant bovine interferon. This novel recombinant interferon exhibited potent antiviral properties (an activity of 1.6 x 10(10) international reference units/mg protein) and may prove to be a valuable reagent in studying the maternal recognition of pregnancy in the cow and reducing embryo mortality.
Peiris
I D
ID
School of Animal and Microbial Sciences, The University of Reading, Whiteknights.
Grewal
T S
TS
Jeacock
M K
MK
Savva
D
D
Shepherd
D A
DA
D000818:Animals; D000998:Antiviral Agents / Q000494:pharmacology*; D001755:Blastocyst / Q000187:drug effects / Q000502:physiology; D002417:Cattle; D004717:Endometrium / Q000187:drug effects / Q000502:physiology*; D005260:Female; D005313:Fetal Death / Q000517:prevention & control / Q000662:veterinary; D007370:Interferon Type I / Q000494:pharmacology*; D007930:Leucine / Q000378:metabolism; D009924:Organ Culture Techniques; D011247:Pregnancy; D011257:Pregnancy Proteins / Q000494:pharmacology*; D011258:Pregnancy Tests / Q000662:veterinary; D011506:Proteins / Q000378:metabolism*; D011994:Recombinant Proteins / Q000494:pharmacology; D012756:Sheep; D014327:Trophoblasts / Q000187:drug effects / Q000502:physiology*
D000998:Antiviral Agents; D007370:Interferon Type I; D011257:Pregnancy Proteins; D011506:Proteins; D011994:Recombinant Proteins; C020947:interferon tau; D007930:Leucine