Cytokine profile associated to effector functions of human T cell clones specific for Schistosoma mansoni antigens.
T cell lines and clones specific for Schistosoma mansoni antigens were established to study cellular immunity in human schistosomiasis. Flow cytometric analysis of the clones demonstrated that all of them were of the helper inducer T cell subset (CD3+, CD4+, CD8-), and expressed the alphabeta T cell receptor, besides the IL-2 low affinity receptor CD25. Lymphokine analysis revealed that clones presented Th1, Th2, or either Th0 patterns of secretion. More interestingly, the capability of clones to induce in vitro granuloma reactions seems to be related to the presence of TNF-alpha and the absence of IL-10. In counterpart, IL-10 producer clones did not help in vitro granuloma formation, even in the presence of TNF-alpha.
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Departamento de Bioquímica e Imunologia, Inst. Ciências Biológicas, Universidade Federal de Minas Gerais, Belo Horizonte, MG, Brazil.
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D000818:Animals; D000947:Antigens, Helminth / Q000276:immunology*; D002460:Cell Line; D002999:Clone Cells; D005434:Flow Cytometry; D006099:Granuloma / Q000276:immunology; D006801:Humans; D016753:Interleukin-10 / Q000096:biosynthesis*; D012550:Schistosoma mansoni / Q000276:immunology*; D050378:T-Lymphocytes, Regulatory / Q000276:immunology* / Q000469:parasitology; D018417:Th1 Cells / Q000276:immunology; D018418:Th2 Cells / Q000276:immunology; D014409:Tumor Necrosis Factor-alpha / Q000096:biosynthesis*
D000947:Antigens, Helminth; D014409:Tumor Necrosis Factor-alpha; D016753:Interleukin-10