title: Filter matrix estimation in automated DNA sequencing.
abstract: In four-color fluourescence-based automated DNA sequencing, a 4 x 4 filter matrix parameterizes the relationship between the dye-intensity signals of interest and the data collected by an optical imaging system. The filter matrix is important because the estimated DNA sequence is based on the dye intensities that can only be recovered via inversion of the matrix. In this paper, we present a calibration method for the estimation of the columns of this matrix, using data generated through a special experiment in which DNA samples are labeled with only one fluorescent dye at a time. Simulations and applications of the method to real data are provided, with promising results.
authors.affiliation: Institute for Biomedical Computing, Washington University, St. Louis, MO 63110, USA.
authors.forename: W
authors.initials: W
authors.lastname: Huang
authors.forename: D R
authors.initials: DR
authors.lastname: Fuhrmann
authors.forename: D G
authors.initials: DG
authors.lastname: Politte
authors.forename: L J
authors.initials: LJ
authors.lastname: Thomas
authors.forename: D J
authors.initials: DJ
authors.lastname: States
mesh_terms: D000465:Algorithms; D004396:Coloring Agents; D003198:Computer Simulation; D007091:Image Processing, Computer-Assisted; D016014:Linear Models; D008957:Models, Genetic; D055095:Optics and Photonics; D011897:Random Allocation; D017422:Sequence Analysis, DNA / Q000379:methods*; D012815:Signal Processing, Computer-Assisted
chemical_list: D004396:Coloring Agents