Effect of oxatomide on T-cell activation and the production of interferon-gamma in mite sensitive asthma.
Interleukin-2 responsiveness of lymphocytes induced by Dermatophagoides farinae antigen was suppressed upon exposure to 20 to 2000 ng/ml of oxatomide for 24 h in a dose-related manner in children with mite-sensitive bronchial asthma. Suppression was greater in the plastic-adherent antigen-presenting cells than in the T-cells. Oxatomide suppressed the production of interleukin-1alpha induced by Dermatophagoides farinae antigen in plastic-adherent cells. These results indicate that the target cells of oxatomide are antigen-presenting cells and not T-cells. Oxatomide also suppressed interleukin-2 responsiveness in lymphocytes exposed to purified protein derivative, but not in those exposed to concanavalin A. Unlike its effect on cell proliferation, oxatomide potentiated the Dermatophagoides farinae-induced production of interferon-gamma, which was suppressed by stimulation with Dermatophagoides farinae antigen in lymphocytes from the patients. In contrast, production of interferon-gamma induced by concanavalin A was not affected by this drug. These results indicate that oxatomide suppresses interleukin-2 responsiveness of allergen-activated helper T-cells and increases the production of interferon-gamma induced by Dermatophagoides farinae antigen, without causing cell proliferation.
Noma
T
T
Department of Pediatrics, School of Medicine, Kitasato University, Sagamihara, Kanagawa, Japan.
Yoshizawa
I
I
Kawano
Y
Y
Nakajima
T
T
D000818:Animals; D018927:Anti-Asthmatic Agents / Q000494:pharmacology*; D039741:Antigens, Dermatophagoides; D002648:Child; D006023:Glycoproteins / Q000276:immunology; D000949:Histocompatibility Antigens Class II / Q000276:immunology; D006801:Humans; D007371:Interferon-gamma / Q000096:biosynthesis*; D007376:Interleukin-2 / Q000096:biosynthesis / Q000276:immunology; D008213:Lymphocyte Activation / Q000187:drug effects*; D008925:Mites / Q000276:immunology*; D009000:Monocytes / Q000187:drug effects / Q000276:immunology; D010879:Piperazines / Q000494:pharmacology*; D013601:T-Lymphocytes / Q000187:drug effects* / Q000276:immunology
D018927:Anti-Asthmatic Agents; D039741:Antigens, Dermatophagoides; D006023:Glycoproteins; D000949:Histocompatibility Antigens Class II; D007376:Interleukin-2; D010879:Piperazines; D007371:Interferon-gamma; C015408:oxatomide