Transformation of Acinetobacter sp. strain BD413 by transgenic sugar beet DNA.
The ability of Acinetobacter sp. strain BD413(pFG4 delta nptII) to take up and integrate transgenic plant DNA based on homologous recombination was studied under optimized laboratory conditions. Restoration of nptII, resulting in kanamycin-resistant transformants, was observed with plasmid DNA, plant DNA, and homogenates carrying the gene nptII. Molecular analysis showed that some transformants not only restored the 317-bp deletion but also obtained additional DNA.
Gebhard
F
F
Biologische Bundesanstalt für Land- und Forstwirtschaft, Institut für Biochemie und Pflanzenvirologie, Braunschweig, Germany.
Smalla
K
K
D000150:Acinetobacter / Q000235:genetics*; D001483:Base Sequence; D019663:Chenopodiaceae / Q000235:genetics* / Q000382:microbiology*; D002874:Chromosome Mapping; D017931:DNA Primers / Q000235:genetics; D018744:DNA, Plant / Q000235:genetics*; D004274:DNA, Recombinant / Q000235:genetics; D018014:Gene Transfer Techniques; D007613:Kanamycin Resistance / Q000235:genetics; D030821:Plants, Genetically Modified; D010957:Plasmids / Q000235:genetics; D016133:Polymerase Chain Reaction; D014170:Transformation, Genetic
D017931:DNA Primers; D018744:DNA, Plant; D004274:DNA, Recombinant