title: Differentiation of oligodendrocyte occurs in contact with astrocyte.
abstract: We have generated stage-specific oligodendrocytes, which are identified as immature oligodendrocytes by the expression of specific markers. These cells were generated by using 3 types of intrinsic and extrinsic factors for influencing the characteristics of oligodendrocyte-lineage cells. The factors included 10% serum in the medium to induce cell proliferation, bFGF in serum-free medium to increase survival and induce differentiation, and 0.25% trypsin to remove nonoligodendrocyte lineage cells. We have also demonstrated that it is possible to supply a huge amount of homogeneous populations of stage-specific immature oligodendrocytes after 35 days' culture. The maturation of these cells is controlled by interacting with astrocytes in vitro, resulting in the expression of MBP isoforms (14.0 and 18.5 kDa) that may participate in mature oligodendrocyte membrane compaction.
authors.affiliation: Department of Neuro-cell biology, Tokyo Metropolitan Institute of Gerontology, Japan.
authors.forename: Y
authors.initials: Y
authors.lastname: Sakurai
authors.forename: D
authors.initials: D
authors.lastname: Nishimura
authors.forename: K
authors.initials: K
authors.lastname: Yoshimura
authors.forename: Y
authors.initials: Y
authors.lastname: Tsuruo
authors.forename: C
authors.initials: C
authors.lastname: Seiwa
authors.forename: H
authors.initials: H
authors.lastname: Asou
mesh_terms: D000818:Animals; D001253:Astrocytes / Q000166:cytology* / Q000502:physiology*; D001921:Brain / Q000166:cytology / Q000502:physiology; D002450:Cell Communication; D002454:Cell Differentiation; D002478:Cells, Cultured; D018920:Coculture Techniques; D004622:Embryo, Mammalian; D004676:Myelin Basic Protein / Q000096:biosynthesis; D009474:Neurons / Q000166:cytology / Q000502:physiology; D009836:Oligodendroglia / Q000166:cytology* / Q000502:physiology*; D016133:Polymerase Chain Reaction; D051381:Rats
chemical_list: D004676:Myelin Basic Protein