Characterization of Oct2 from the channel catfish: functional preference for a variant octamer motif.
The Ig heavy chain enhancer of the channel catfish (Ictalurus punctatus) has an unusual position and structure, being found in the 3' region of the mu gene and containing eight functional octamer motifs of consensus (ATGCAAAT) and variant sequences. The presence of multiple octamer motifs suggests that an Oct2 homologue may play an important role in driving expression of the Ig heavy chain locus in a teleost fish. To test this hypothesis, two catfish Oct2 cDNAs (alpha and beta) were cloned by screening a catfish B cell cDNA library. Catfish Oct2 alpha and beta isoforms are derived by alternative RNA splicing; as determined by Southern analysis, Oct2 is a single copy gene. In comparisons with mammalian Oct2, the catfish Oct2 isoforms show high sequence conservation in their N-terminal regions and POU domains, but extensive divergence in their C-terminal regions. Catfish Oct2 a and beta are tissue restricted, bind both consensus and variant octamer motifs, and activate transcription in both catfish and murine cells. In contrast, mouse Oct2 activated transcription in mouse but not catfish cells. Catfish Oct2 beta is a more potent transcriptional activator than Oct2 alpha. In transient expression assays, catfish Oct2 beta showed a marked preference for the octamer variant, ATGtAAAT, which occurs twice in the catfish enhancer. Mouse Oct2 also showed increased activity with the variant octamer when tested in mouse B cells. Gel-shift analysis competition assays indicated that catfish Oct2 binds the consensus octamer motif with an apparently higher affinity than it does the variant motif.
Ross
D A
DA
Department of Biochemistry and Molecular Biology, Medical University of South Carolina, Charleston 29425, USA.
Magor
B G
BG
Middleton
D L
DL
Wilson
M R
MR
Miller
N W
NW
Clem
L W
LW
Warr
G W
GW
D017398:Alternative Splicing; D000595:Amino Acid Sequence; D000818:Animals; D001402:B-Lymphocytes / Q000276:immunology; D001483:Base Sequence; D004247:DNA / Q000235:genetics / Q000378:metabolism; D015342:DNA Probes / Q000235:genetics; D004268:DNA-Binding Proteins; D015870:Gene Expression; D005803:Genes, Immunoglobulin; D014644:Genetic Variation; D007059:Ictaluridae / Q000235:genetics* / Q000276:immunology* / Q000378:metabolism; D008297:Male; D051379:Mice; D008969:Molecular Sequence Data; D050813:Octamer Transcription Factor-2; D012333:RNA, Messenger / Q000235:genetics; D017386:Sequence Homology, Amino Acid; D014018:Tissue Distribution; D014157:Transcription Factors / Q000235:genetics* / Q000276:immunology* / Q000378:metabolism
D015342:DNA Probes; D004268:DNA-Binding Proteins; D050813:Octamer Transcription Factor-2; C489206:Pou2f2 protein, mouse; D012333:RNA, Messenger; D014157:Transcription Factors; D004247:DNA