Effect of inhibitors of enzymes involved in polyamine biosynthesis pathway on pregnancy in mouse and hamster.
The syntheses of polyamines, which play a definitive role in cell proliferation and tissue growth during early embryogenesis, are believed to be principally regulated by two enzymes: ornithine decarboxylase (ODC) and polyamine oxidase (PAO). However, when the level of enzyme ODC goes down, another enzyme known as S-adenosyl methyl decarboxylase (SAMDC) is believed to up-regulate the biosynthetic pathway. In the present study, the effects of inhibitors of enzymes SAMDC and PAO were determined in mated hamster and mouse in peri-and immediate postimplantation stages of pregnancy. It was noticed that, whereas the SAMDC inhibitor (MDL-73815) failed to arrest the pregnancy in the hamster in spite of raising the dose to 100 mg/kg, the PAO inhibitor (MDL-72527DA) was found to be 100% effective at 75 mg/kg in both species. The results confirmed the role of enzyme PAO in promoting early development of the embryo.
Mehrotra
P K
PK
Division of Endocrinology, Central Drug Research Institute, Lucknow, India. Mehrotraroot@cscdri.ren.nic.in
Kitchlu
S
S
Farheen
S
S
D012437:Adenosylmethionine Decarboxylase / Q000037:antagonists & inhibitors*; D000818:Animals; D006224:Cricetinae; D004305:Dose-Response Relationship, Drug; D010064:Embryo Implantation / Q000187:drug effects; D004791:Enzyme Inhibitors / Q000008:administration & dosage / Q000494:pharmacology*; D005260:Female; D007279:Injections, Subcutaneous; D008297:Male; D008647:Mesocricetus; D051379:Mice; D009930:Organic Chemicals; D000587:Oxidoreductases Acting on CH-NH Group Donors / Q000037:antagonists & inhibitors*; D011247:Pregnancy; D011270:Pregnancy, Animal / Q000187:drug effects*; D011700:Putrescine / Q000008:administration & dosage / Q000031:analogs & derivatives* / Q000494:pharmacology; D013997:Time Factors; D014327:Trophoblasts / Q000187:drug effects* / Q000502:physiology; D014599:Uterus / Q000187:drug effects; D000097667:Polyamine Oxidase
D012437:Adenosylmethionine Decarboxylase; D004791:Enzyme Inhibitors; D009930:Organic Chemicals; D000587:Oxidoreductases Acting on CH-NH Group Donors; D011700:Putrescine; D000097667:Polyamine Oxidase; C112284:MDL 73815; C044445:MDL 72527