Expression of HSP 70 as a potential prognostic marker for acute rejection in human liver transplantation.
In experimental models, the synthesis of heat shock protein 70 (HSP 70) has been recognized as an intracellular response to ischemia and reperfusion, insults inherent to transplantation. In this study, the HSP response in early stages of human liver transplantation was investigated. HSP 70 mRNA expression was detected by means of reverse transcriptase (RT)-PCR in liver biopsies (n = 28) and in cells obtained from the organ perfusate (n = 14) following cold preservation. The expression of HSP 70 differed substantially between individuals. Retrospective analysis revealed a close correlation of the amount of HSP 70 mRNA in perfusate cells and biopsies with the onset of organ dysfunction due to early graft rejection. Patients with early graft rejection had a significantly lower amount of HSP 70 mRNA than patients without rejection. These results suggest a protective role of HSP 70 expression. Low levels of HSP 70 may, therefore, represent a prognostic marker for early graft rejection.
Flohé
S
S
University Hospital of Essen, Clinical Research Group Shock & MOF, Germany.
Speidel
N
N
Flach
R
R
Lange
R
R
Erhard
J
J
Schade
F U
FU
D000328:Adult; D015415:Biomarkers; D001706:Biopsy; D005260:Female; D006084:Graft Rejection / Q000175:diagnosis* / Q000378:metabolism; D018840:HSP70 Heat-Shock Proteins / Q000032:analysis / Q000096:biosynthesis*; D006801:Humans; D015850:Interleukin-6 / Q000032:analysis / Q000096:biosynthesis; D016209:Interleukin-8 / Q000032:analysis / Q000096:biosynthesis; D016031:Liver Transplantation / Q000473:pathology; D008297:Male; D008875:Middle Aged; D016133:Polymerase Chain Reaction; D011379:Prognosis; D012333:RNA, Messenger / Q000032:analysis
D015415:Biomarkers; D018840:HSP70 Heat-Shock Proteins; D015850:Interleukin-6; D016209:Interleukin-8; D012333:RNA, Messenger