Molecular cloning and expression of the canine metallothionein-III gene.
We have isolated and determined the complete nucleotide sequence of canine metallothionein-III (MT-III) cDNA. The predicted amino acid sequence of the canine MT-III showed a high homology (93%, 87% identity) to that of human and mouse MT-III. The canine MT-III had 2 insertions relative to known mammalian MT-I and MT-II: a threonine after the 4th amino acid and a block of 6 amino acids near the carboxyl terminus. Expression of the canine MT-III mRNA was found exclusively in the central nervous system, where neurons in the olfactory bulb, hippocampus and cerebral cortex showed predominant signals.
Kojima
S
S
Department of Veterinary Pathology, Tottori University, Japan.
Shimada
A
A
Kodan
A
A
Kobayashi
K
K
Morita
T
T
Yamano
Y
Y
Umemura
T
T
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D001921:Brain / Q000378:metabolism*; D004251:DNA Transposable Elements; D004285:Dogs; D006801:Humans; D008322:Mammals; D008668:Metallothionein / Q000096:biosynthesis* / Q000737:chemistry / Q000235:genetics*; D051379:Mice; D008969:Molecular Sequence Data; D009474:Neurons / Q000378:metabolism*; D009928:Organ Specificity; D012333:RNA, Messenger / Q000032:analysis; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000737:chemistry; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid
D004251:DNA Transposable Elements; D012333:RNA, Messenger; D011994:Recombinant Proteins; D008668:Metallothionein