Exoprotein and slime production by coagulase-negative staphylococci isolated from goats' milk.
Milk from mastitis-free goats from French herds was examined for the presence of coagulase-negative staphylococci (CNS), and 165 positive isolates were evaluated for their capacity to produce exoproteins. Most isolates were identified as Staphylococcus caprae (N = 91) or S. xylosus (N = 36), but members of at least nine other species were present. Overall, some 57% of isolates produced toxins with phenotypic properties of alpha-hemolysin, and 75% produced toxins resembling S. aureus beta and/or delta-hemolysins. Thermostable desoxyribonuclease (TNAse) was secreted by 29% of isolates and thermolabile DNAse by 66%. Slime was produced by 42% of our cultures and, although none of them showed activity to insoluble elastase, over 70% lysed the soluble substrate. No clinical consequences were observed to correlate with exoprotein production, which proved to be inconstant within individual CNS species.
Bedidi-Madani
N
N
Département de Santé Publique et Hygiéne des Produits Animaux, Ecole Vétérinaire de Lyon, Marcy L'Etoile, France.
Greenland
T
T
Richard
Y
Y
D000818:Animals; D001426:Bacterial Proteins / Q000096:biosynthesis*; D015169:Colony Count, Microbial / Q000662:veterinary; D003851:Deoxyribonucleases / Q000032:analysis / Q000096:biosynthesis; D005098:Exotoxins / Q000096:biosynthesis; D005260:Female; D005602:France; D015511:Goat Diseases / Q000382:microbiology*; D006041:Goats; D006460:Hemolysin Proteins / Q000096:biosynthesis; D006461:Hemolysis / Q000502:physiology; D008413:Mastitis / Q000382:microbiology / Q000662:veterinary*; D008892:Milk / Q000737:chemistry / Q000382:microbiology*; D010196:Pancreatic Elastase / Q000096:biosynthesis; D012260:Ribonucleases / Q000032:analysis / Q000096:biosynthesis; D013203:Staphylococcal Infections / Q000473:pathology / Q000662:veterinary*; D013210:Staphylococcus / Q000378:metabolism / Q000472:pathogenicity*; D014774:Virulence
D001426:Bacterial Proteins; D005098:Exotoxins; D006460:Hemolysin Proteins; D003851:Deoxyribonucleases; D012260:Ribonucleases; D010196:Pancreatic Elastase