title: Identification of target cells by immunohistochemical detection of covalently rearranged estradiol in rehydrated paraffin sections.
abstract: Estradiol is released from the binding niche of the receptor and covalently arrested in the molecular vicinity by the Mannich reaction during target fixation in acetic acid/formaldehyde. The exposed steroid is freely accessible for appropriate antibodies. It can be visualized in sections by the second antibody/enzyme technique in high resolution and without enhancements.
authors.affiliation: Max-Planck-Institut für Experimentelle Endokrinologie, Hannover, Germany. 106001,2503@compuserve.com
authors.forename: P W
authors.initials: PW
authors.lastname: Jungblut
authors.forename: W D
authors.initials: WD
authors.lastname: Sierralta
mesh_terms: D000818:Animals; D001940:Breast / Q000378:metabolism; D004717:Endometrium / Q000378:metabolism; D004958:Estradiol / Q000008:administration & dosage / Q000378:metabolism*; D005187:Fallopian Tubes / Q000378:metabolism; D005260:Female; D006041:Goats; D006801:Humans; D007124:Immunoenzyme Techniques; D008867:Microtomy; D016612:Paraffin Embedding; D017698:Postmenopause; D051381:Rats; D017207:Rats, Sprague-Dawley; D013552:Swine; D013977:Tibia / Q000378:metabolism
chemical_list: D004958:Estradiol