Polypyrimidine tract-binding protein interacts with HnRNP L.
Polypyrimidine tract-binding protein (PTB) is involved in pre-mRNA splicing and internal ribosomal entry site (IRES)-dependent translation. In order to identify cellular protein(s) interacting with PTB, we performed a yeast two-hybrid screening. Heterogeneous nuclear ribonucleoprotein L (hnRNP L) was identified as a PTB-binding protein. The interaction between PTB and hnRNP L was confirmed in an in vitro binding assay. Both PTB and hnRNP L were found to localize in the nucleoplasm, excepting the nucleoli, in HeLa cells by the green fluorescent protein (GFP)-fused protein detection method. The N-terminal half of PTB (aa 1-329) and most of hnRNP L (aa 141-558) is required for the interaction between PTB and hnRNP L.
Hahm
B
B
Department of Life Science, Pohang University of Science and Technology, Kyungbuk, South Korea.
Cho
O H
OH
Kim
J E
JE
Kim
Y K
YK
Kim
J H
JH
Oh
Y L
YL
Jang
S K
SK
D004268:DNA-Binding Proteins / Q000378:metabolism*; D017930:Genes, Reporter / Q000235:genetics; D049452:Green Fluorescent Proteins; D006367:HeLa Cells; D034664:Heterogeneous-Nuclear Ribonucleoprotein L; D034441:Heterogeneous-Nuclear Ribonucleoproteins; D006801:Humans; D008164:Luminescent Proteins / Q000235:genetics; D008856:Microscopy, Fluorescence; D009687:Nuclear Proteins / Q000378:metabolism; D038941:Polypyrimidine Tract-Binding Protein; D014176:Protein Biosynthesis / Q000235:genetics; D012322:RNA Precursors / Q000378:metabolism; D012326:RNA Splicing / Q000502:physiology; D016601:RNA-Binding Proteins / Q000378:metabolism*; D011993:Recombinant Fusion Proteins / Q000378:metabolism; D012261:Ribonucleoproteins / Q000378:metabolism*; D017384:Sequence Deletion / Q000235:genetics; D001616:beta-Galactosidase / Q000235:genetics / Q000378:metabolism
D004268:DNA-Binding Proteins; D034664:Heterogeneous-Nuclear Ribonucleoprotein L; D034441:Heterogeneous-Nuclear Ribonucleoproteins; D008164:Luminescent Proteins; D009687:Nuclear Proteins; D012322:RNA Precursors; D016601:RNA-Binding Proteins; D011993:Recombinant Fusion Proteins; D012261:Ribonucleoproteins; D038941:Polypyrimidine Tract-Binding Protein; D049452:Green Fluorescent Proteins; D001616:beta-Galactosidase