Randomly ordered addressable high-density optical sensor arrays.
Array-based sensors provide an architecture for multianalyte sensing. In this paper, we report a new approach for array fabrication. Sensors are made by immobilizing different reactive chemistries on the surfaces of microspheres. Sensor arrays are prepared by randomly distributing a mixture of microsphere sensors on an optical substrate containing thousands of micrometer-scale wells. The sensors occupy a different location from array to array; thus the identity of each sensor is ascertained and registered on the detector using encoding schemes, rather than by a predetermined location in the array. The approach thereby shifts the demand from fabrication to signal processing. The availability of commercial image analysis software makes such a shift both cost-effective and time efficient.
Michael
K L
KL
Max Tishler Laboratory for Organic Chemistry, Department of Chemistry, Tufts University, Medford, Massachusetts 02155, USA.
Taylor
L C
LC
Schultz
S L
SL
Walt
D R
DR
D003362:Cost-Benefit Analysis; D005336:Fiber Optic Technology; D005456:Fluorescent Dyes; D018625:Microscopy, Atomic Force / Q000379:methods*; D008863:Microspheres
D005456:Fluorescent Dyes