title: Combined effect of ampicillin, colistin and dexamethasone administered intramuscularly to dairy cows on the clinico-pathological course of E. coli-endotoxin mastitis.
abstract: The effects of a single intramuscular injection of a drug product containing ampicillin, colistin and dexamethasone, as a suspension in a diester of propylene glycol of medium-chain fatty acids, on the clinico-pathological course of experimental Escherichia coli-endotoxin mastitis was examined in 30 dairy cows. Cows were divided into five groups, six cows per group, and 24 of them were infused with E. coli endotoxin into two quarters of their udders. The drug product was injected at 25,000 IU colistin sulphate, 10.0 mg ampicillin anhydrate and 0.025 mg dexamethasone acetate.kg-1 body weight as follows: Group 2 cows, immediately post-endotoxin infusion (PEI); Group 3 cows, 2 h PEI and, Group 4 cows, 4 h PEI. Group 1 cows were not treated with the product and served as a positive (endotoxin only) control while Group 5 cows were not challenged with endotoxin and were not treated with the product. A clinical mastitis score (CMS) was developed to quantitatively assess the degree of inflammation. Blood biochemistry and hematological parameters were used to monitor the immediate effects of treatment on several conventional inflammatory markers. Milk somatic cell counts (MSCC), milk electrical conductivity and daily milk production were among the parameters used to monitor systemic and local inflammatory reactions. Administration of the drug product immediately PEI and 2 h PEI clearly nullified some of the most severe early systemic reactions to inflammation but the effect of therapy on the local inflammatory markers was not as obvious. Notewhorthy, however, were the effects of the treatment on reducing the duration of elevated quarter MSCC and the increase in the speed of return to pre-endotoxin challenge daily milk production levels.
authors.affiliation: VETGENERICS LTD, Rehovot, Israel.
authors.forename: G
authors.initials: G
authors.lastname: Ziv
authors.forename: M
authors.initials: M
authors.lastname: Shem-Tov
authors.forename: F
authors.initials: F
authors.lastname: Ascher
mesh_terms: D000667:Ampicillin / Q000008:administration & dosage / Q000627:therapeutic use*; D000818:Animals; D002118:Calcium / Q000097:blood; D002417:Cattle; D002418:Cattle Diseases; D003091:Colistin / Q000008:administration & dosage / Q000627:therapeutic use*; D003907:Dexamethasone / Q000008:administration & dosage / Q000627:therapeutic use*; D004359:Drug Therapy, Combination / Q000008:administration & dosage / Q000627:therapeutic use*; D004731:Endotoxins; D004927:Escherichia coli Infections / Q000188:drug therapy / Q000503:physiopathology / Q000662:veterinary*; D005260:Female; D007273:Injections, Intramuscular; D007770:L-Lactate Dehydrogenase / Q000097:blood; D007774:Lactation / Q000187:drug effects / Q000502:physiology*; D007958:Leukocyte Count; D008414:Mastitis, Bovine / Q000188:drug therapy* / Q000382:microbiology* / Q000503:physiopathology; D008892:Milk / Q000378:metabolism; D009119:Muscle Contraction / Q000187:drug effects; D012417:Rumen / Q000502:physiology / Q000503:physiopathology
chemical_list: D004731:Endotoxins; D000667:Ampicillin; D003907:Dexamethasone; D007770:L-Lactate Dehydrogenase; D002118:Calcium; D003091:Colistin