Study on lipid peroxidation potential in different tissues induced by ascorbate-Fe2+: possible factors involved in their differential susceptibility.
Susceptibility of four major rat tissues to oxidative damage in terms of lipid peroxidation induced by in vitro by ascorbate-Fe2+ in homogenates and mitochondria has been examined. Lipid peroxidation, as assessed by thiobarbituric acid reactive substances (TBARS) and conjugated dienes was maximum in brain followed by liver, kidney and heart. However, the time course of lipid peroxidation showed different patterns in tissues examined. The higher susceptibilities of brain and liver can be explained by substrate availability and to a lesser extent the level of antioxidants. The differences observed in the tissues studied may reflect their susceptibility to degenerative diseases and xenobiotic toxicity which are considered as a result of oxidative damage to membranes.
Pushpendran
C K
CK
Radiation Biology and Biochemistry Division, Bhabha Atomic Research Centre, Mumbai, India.
Subramanian
M
M
Devasagayam
T P
TP
Singh
B B
BB
D000818:Animals; D000970:Antineoplastic Agents / Q000494:pharmacology; D001205:Ascorbic Acid / Q000494:pharmacology*; D001921:Brain / Q000187:drug effects / Q000378:metabolism; D005260:Female; D005296:Ferrous Compounds / Q000494:pharmacology*; D006321:Heart / Q000187:drug effects; D007668:Kidney / Q000187:drug effects / Q000378:metabolism; D008054:Lipid Peroxides / Q000378:metabolism*; D008099:Liver / Q000187:drug effects / Q000378:metabolism; D009206:Myocardium / Q000378:metabolism; D018384:Oxidative Stress; D051381:Rats; D017208:Rats, Wistar; D013482:Superoxide Dismutase / Q000378:metabolism; D017392:Thiobarbituric Acid Reactive Substances / Q000378:metabolism
D000970:Antineoplastic Agents; D005296:Ferrous Compounds; D008054:Lipid Peroxides; D017392:Thiobarbituric Acid Reactive Substances; C051140:iron(II)-ascorbic acid complex; D013482:Superoxide Dismutase; D001205:Ascorbic Acid