Ultraspatially-resolved synchrotron infrared microspectroscopy of plant tissue in situ.
Routine use of 6 microm or 12 microm apertures with synchrotron microspectroscopy provide good spectra without excessive co-addition of scans. 100% mapping by stepping in pixel sized increments reveals chemical heterogeneity within cellular dimensions. The brightness of the synchrotron source and the absence of thermal noise compared to a conventional thermal (globar) source yields favorable signal-to-noise operation. The nondivergent characteristics of the source result in minimal loss of radiation at the aperture, hence, spatial resolution approaches the diffraction limit. Details of cellular dimensions are then localized within any maps produced and individual spectra obtained from adjacent pixels clearly shows the striking difference in chemistry even within a microscopic vicinity. In this report the mapping of plant tissue with the synchrotron is contrasted to previous lower spatial resolution mapping experiments done with the globar on similar materials using interpolation between separated sampling spots and larger apertures.
Wetzel
D L
DL
Microbeam Molecular Spectroscopy Laboratory, Kansas State University, Manhattan 66506, USA.
Eilert
A J
AJ
Pietrzak
L N
LN
Miller
S S
SS
Sweat
J A
JA
D000042:Absorption; D018554:Avena; D005936:Glucans / Q000032:analysis; D008867:Microtomy; D018517:Plant Roots / Q000648:ultrastructure; D010944:Plants / Q000648:ultrastructure*; D006109:Poaceae; D013055:Spectrophotometry, Infrared / Q000379:methods*; D017356:Synchrotrons; D014908:Triticum / Q000648:ultrastructure; D003313:Zea mays / Q000648:ultrastructure
D005936:Glucans