title: Immunotoxicology of cigarette smoke condensates: suppression of macrophage responsiveness to interferon gamma.
abstract: We have investigated systematically the effects of short-term exposure to main stream cigarette smoke condensates (CSC-MS) on basal and inducible functional capacities of murine peritoneal exudate macrophages. Macrophages treated with CSC-MS form granules that fluoresce orange under blue excitation, consistent with the speculation that they are polycyclic aromatic hydrocarbons (PAH). CSC-MS selectively suppressed interferon gamma (IFN gamma) induction of four macrophage functional capacities: enhanced phagocytosis of immunoglobulin-opsonized sheep red blood cells, TPA-induced H2O2 production, class II major histocompatibility complex expression, and nitric oxide synthesis. In contrast, two macrophage functions that are not induced by IFN gamma, basal electron transport and LPS-induced TNF alpha production, were enhanced by treatment with CSC-MS. These results suggest that the suppressive effects of CSC-MS on macrophage responsiveness were selective and were not due to nonspecific inhibition of general functions such as RNA or protein synthesis. Since macrophage responsiveness to IFN gamma can result in induction of functional capacities that are fundamental to immunity, the data suggest that CSC-MS maybe deleterious to the general health of the smoker.
authors.affiliation: Department of Biology, Bradley University, Peoria, Illinois 61625, USA.
authors.forename: K M
authors.initials: KM
authors.lastname: Braun
authors.forename: T
authors.initials: T
authors.lastname: Cornish
authors.forename: A
authors.initials: A
authors.lastname: Valm
authors.forename: J
authors.initials: J
authors.lastname: Cundiff
authors.forename: J L
authors.initials: JL
authors.lastname: Pauly
authors.forename: S
authors.initials: S
authors.lastname: Fan
mesh_terms: D000818:Animals; D002273:Carcinogens / Q000009:adverse effects; D004579:Electron Transport; D005260:Female; D000949:Histocompatibility Antigens Class II / Q000096:biosynthesis; D007111:Immunity, Cellular / Q000187:drug effects; D007371:Interferon-gamma / Q000494:pharmacology*; D008070:Lipopolysaccharides; D017737:Macrophages, Peritoneal / Q000187:drug effects* / Q000276:immunology* / Q000378:metabolism; D051379:Mice; D008810:Mice, Inbred C57BL; D009569:Nitric Oxide / Q000096:biosynthesis; D010587:Phagocytosis / Q000187:drug effects; D011084:Polycyclic Aromatic Hydrocarbons / Q000009:adverse effects; D016897:Respiratory Burst; D014028:Tobacco Smoke Pollution / Q000009:adverse effects*; D014409:Tumor Necrosis Factor-alpha / Q000096:biosynthesis
chemical_list: D002273:Carcinogens; D000949:Histocompatibility Antigens Class II; D008070:Lipopolysaccharides; D011084:Polycyclic Aromatic Hydrocarbons; D014028:Tobacco Smoke Pollution; D014409:Tumor Necrosis Factor-alpha; D009569:Nitric Oxide; D007371:Interferon-gamma