title: Vi-deficient and nonfimbriated mutants of Salmonella typhi agglutinate human blood type antigens and are hyperinvasive.
abstract: We generated nonfimbriated mutants from both Vi-positive and -negative Salmonella typhi to analyze the role of type 1 fimbriae and Vi-antigen in bacterial invasion. A Vi-defective mutant of S. typhi GIFU 10007-3 was more invasive than the wild-type strain GIFU 10007. The wild-type strain expressing Vi-antigen did not agglutinate both Saccharomyces cerevisiae and human erythrocytes but Vi-defective mutants were able to agglutinate S. cerevisiae and human erythrocytes. Nonfimbriated mutants from Vi-negative GIFU 10007-3 lost the ability to adhere to S. cerevisiae but still could agglutinate human erythrocytes. The Vi-negative mutant increased secreted proteins and became 5-fold more invasive than the wild-type strain. Nonfimbriated Vi mutants became 50-120-fold more invasive than the wild-type GIFU 10007. To determine why nonfimbriated Vi mutants still agglutinate human red blood cells, we searched bacterial proteins that could bind human blood-type antigens. We finally identified a candidate 37 kDa outer membrane protein that recognized fucosyl-galactose, a structure common to blood type A, B and H antigens.
authors.affiliation: Department of Microbiology, School of Pharmaceutical Sciences, University of Shizuoka, Japan.
authors.forename: M
authors.initials: M
authors.lastname: Miyake
authors.forename: L
authors.initials: L
authors.lastname: Zhao
authors.forename: T
authors.initials: T
authors.lastname: Ezaki
authors.forename: K
authors.initials: K
authors.lastname: Hirose
authors.forename: A Q
authors.initials: AQ
authors.lastname: Khan
authors.forename: Y
authors.initials: Y
authors.lastname: Kawamura
authors.forename: R
authors.initials: R
authors.lastname: Shima
authors.forename: M
authors.initials: M
authors.lastname: Kamijo
authors.forename: T
authors.initials: T
authors.lastname: Masuzawa
authors.forename: Y
authors.initials: Y
authors.lastname: Yanagihara
mesh_terms: D000017:ABO Blood-Group System / Q000276:immunology*; D000371:Agglutination; D000942:Antigens, Bacterial / Q000502:physiology*; D001425:Bacterial Outer Membrane Proteins / Q000378:metabolism; D010861:Fimbriae, Bacterial / Q000502:physiology*; D006801:Humans; D009154:Mutation; D011135:Polysaccharides, Bacterial / Q000502:physiology*; D012485:Salmonella typhi / Q000502:physiology*
chemical_list: D000017:ABO Blood-Group System; D000942:Antigens, Bacterial; D001425:Bacterial Outer Membrane Proteins; D011135:Polysaccharides, Bacterial