Adhesion molecules in intestinal Schistosoma mansoni infection.
Adhesion molecules constitute essential elements in inflammation, mediating various cellular interactions. We investigated the expression of adhesion molecules mediating cell-cell [intercellular adhesion molecule-1 (ICAM-1) and lymphocyte function-associated antigen-1 (LFA-1)] and cell-matrix interactions [very late antigen-4 (VLA-4), VLA-6, and syndecan-1] in intestinal granulomas of mice infected with the parasite Schistosoma mansoni. Up-regulation of ICAM-1, LFA-1, and VLA-4 was seen in ileal and colonic granulomas, at both the acute (8 weeks postinfection) and the chronic stage (13-16 weeks postinfection). Up-regulation of VLA-6 was absent in all intestinal granulomas. Syndecan-1 immunoreactive (antigen-driven) B-lymphocytes were seen in the proximity of egg-antigen-laden macrophages in the inner part of ileal and colonic granulomas, although B-cells are considered to be absent in ileal granulomas. Estimation of intestinal granuloma volumes demonstrated the lack of down-modulation observed in ileal granulomas. From our results we infer that adhesion molecules constitute important elements in schistosomal intestinal granuloma formation. Organ-related differences between hepatic and intestinal granulomas exist (e.g., granuloma volume), but these differences are not morphologically reflected in a differential expression of the adhesion molecules ICAM-1, LFA-1, and VLA-4. Syndecan-1 immunoreactive B-lymphocytes also appear to be involved in ileal granuloma formation.
Jacobs
W
W
Department of Pathology, University of Antwerp, Wilrijk, Belgium.
Bogers
J J
JJ
Timmermans
J P
JP
Deelder
A M
AM
Van Marck
E A
EA
D000818:Animals; D000947:Antigens, Helminth / Q000032:analysis; D015815:Cell Adhesion Molecules / Q000032:analysis*; D003106:Colon / Q000737:chemistry / Q000473:pathology; D006099:Granuloma / Q000378:metabolism* / Q000473:pathology; D007082:Ileum / Q000737:chemistry / Q000473:pathology; D007150:Immunohistochemistry; D039041:Integrin alpha4beta1; D039121:Integrin alpha6beta1; D016023:Integrins / Q000032:analysis; D018799:Intercellular Adhesion Molecule-1 / Q000032:analysis; D016169:Lymphocyte Function-Associated Antigen-1 / Q000032:analysis; D008297:Male; D008562:Membrane Glycoproteins / Q000032:analysis; D051379:Mice; D011509:Proteoglycans / Q000032:analysis; D016201:Receptors, Lymphocyte Homing / Q000032:analysis; D012555:Schistosomiasis mansoni / Q000378:metabolism* / Q000473:pathology; D053668:Syndecan-1; D053667:Syndecans; D015854:Up-Regulation
D000947:Antigens, Helminth; D015815:Cell Adhesion Molecules; D039041:Integrin alpha4beta1; D039121:Integrin alpha6beta1; D016023:Integrins; D016169:Lymphocyte Function-Associated Antigen-1; D008562:Membrane Glycoproteins; D011509:Proteoglycans; D016201:Receptors, Lymphocyte Homing; C508242:Sdc1 protein, mouse; D053668:Syndecan-1; D053667:Syndecans; D018799:Intercellular Adhesion Molecule-1