Effects of fumonisin B1 on lipid peroxidation in membranes.
Electron spin resonance (ESR)1 spin-label oximetry and spin trapping techniques have been used to study the effect of fumonisin B1 (FB1), an amphipathic mycotoxin on lipid peroxidation in egg yolk phosphatidylcholine (EYPC) bilayers. In the study of the interaction between FB1 and lipid bilayers our results show that fumonisin disturbs the ordering of membranes, enhances oxygen transport in membranes, and also increases membrane permeability. In our model system, lipid peroxidations were initiated by extended incubation of the liposomes, or by inducing Fe2+ ions, UV illumination of H2O2 or ultrasound irradiation. As an indication of the rates of lipid oxidation in EYPC, the consumption of molecular oxygen was studied by monitoring the oxygen concentration in the aqueous phases of the liposomes. Lipid-derived free radicals generated during the oxidation process were measured by a spin trapping method. The incorporation of FB1 in the test samples made the membranes highly susceptible to oxidation. Our results provide the first evidence that the fumonisins appear to increase the rate of oxidation, promote the free radical intermediate production and accelerate the chain reactions associated with lipid peroxidation. The disruption of membrane structure, the enlargement of the relative oxygen diffusion-concentration products, as well as the enhancement effects on membrane permeability, thus provide additional insights into potential mechanisms by which the fumonisins could enhance oxidative stress and cell damage.
Yin
J J
JJ
Center for Food Safety and Applied Nutrition, Food and Drug Administration, Washington, DC 20204, USA.
Smith
M J
MJ
Eppley
R M
RM
Page
S W
SW
Sphon
J A
JA
D002264:Carboxylic Acids / Q000494:pharmacology*; D002413:Cations, Divalent; D002463:Cell Membrane Permeability / Q000187:drug effects; D004530:Egg Yolk; D004578:Electron Spin Resonance Spectroscopy; D037341:Fumonisins; D006861:Hydrogen Peroxide; D007501:Iron; D015227:Lipid Peroxidation / Q000187:drug effects*; D008081:Liposomes / Q000378:metabolism*; D009183:Mycotoxins / Q000494:pharmacology*; D010101:Oxygen Consumption; D010713:Phosphatidylcholines; D019097:Spin Trapping; D014465:Ultrasonics; D014466:Ultraviolet Rays
D002264:Carboxylic Acids; D002413:Cations, Divalent; D037341:Fumonisins; D008081:Liposomes; D009183:Mycotoxins; D010713:Phosphatidylcholines; C056933:fumonisin B1; D006861:Hydrogen Peroxide; D007501:Iron