[DNA inside bacteriophage lambda forms a Z-form].
The site of the transition regions between the B- and Z-forms of the DNA duplex (B-Z junction) may serve as marker of the existence of Z-DNA in situ. The structure of (dC-dG)10 insert in the bacteriophage lambda gt10 has been studied in situ by modification of B-Z junction with O-beta-diethylaminoethylhydroxylamine (OHA). The latter is an analogue of hydroxylamine possessing specificity with respect to unpaired cytidine. This modification inhibited the process of restriction at BamHI site adjacent to the Z-insert. Judging by the extent of the inhibition about 5% of all inserts has been converted in Z-form. The certain role of Z-form at process of the packaging DNA into bacteriophage's capsid suggest.
Ulanov
B P
BP
Emanuel Institute of Biochemical Physics, Russian Academy of Sciences, Moscow, Russia.
Matorina
T I
TI
Bogush
A I
AI
D010582:Bacteriophage lambda / Q000235:genetics*; D004279:DNA, Viral / Q000737:chemistry*; D006898:Hydroxylamines / Q000737:chemistry; D009690:Nucleic Acid Conformation; D015183:Restriction Mapping
D004279:DNA, Viral; D006898:Hydroxylamines; C015133:O-beta-diethylaminoethylhydroxylamine