Ligation of the beta2 integrin triggers activation and degranulation of human eosinophils.
Evidence suggests that cellular adhesion is critical for eosinophil effector functions. Here, we tested the hypothesis that an adhesion molecule, specifically beta2 integrin, participates in intracellular signaling events of eosinophils. Eosinophils stimulated with interleukin (IL)-5 and adherent to protein-coated tissue culture plates via beta2 integrin (CD18) showed tyrosine phosphorylation of a number of proteins. Among these proteins, tyrosine phosphorylation of the 105 kD and 115 kD proteins and the product of the c-cbl protooncogene, Cbl, was specifically inhibited using soluble anti-CD18 monoclonal antibody (mAb) to block eosinophil cell adhesion. Furthermore, phosphoinositide turnover of IL-5-stimulated adherent eosinophils was also inhibited by anti-CD18 mAb, suggesting that cellular adhesion plays important roles in eosinophil signal transduction. alphaM beta2 (Mac-1, CD11b/18) was one of the beta2 integrins involved in eosinophil adhesion to protein-coated plates. We found that direct ligation of eosinophil alphaM beta2 with anti-CD11b mAb coupled to polystyrene microbeads induced tyrosine phosphorylation of a 115 kD protein and Cbl. Furthermore, anti-CD11b mAb microbeads induced increases in both phosphoinositide hydrolysis and the eosinophil degranulation response. Control antibodies, such as mouse myeloma IgG1 and anti-HLA class I antigen mAb, did not induce these cellular responses. These results suggest that engagement of beta2 integrin either by cell adhesion or by anti-CD11b mAb triggers activation of an intracellular signaling cascade, including protein tyrosine phosphorylation and phosphoinositide turnover, and subsequent cellular degranulation in human eosinophils. Tyrosine phosphorylation of a 115 kD protein and Cbl may play important roles in adhesion-dependent cellular functions of eosinophils.
Kato
M
M
Department of Immunology, Mayo Clinic and Mayo Foundation, Rochester, Minnesota, USA.
Abraham
R T
RT
Okada
S
S
Kita
H
H
D000911:Antibodies, Monoclonal / Q000494:pharmacology; D018821:CD18 Antigens / Q000276:immunology* / Q000378:metabolism*; D002448:Cell Adhesion / Q000276:immunology; D003594:Cytoplasmic Granules / Q000276:immunology*; D004804:Eosinophils / Q000166:cytology / Q000276:immunology* / Q000378:metabolism; D006801:Humans; D015848:Interleukin-5 / Q000276:immunology / Q000494:pharmacology; D008024:Ligands; D016177:Macrophage-1 Antigen / Q000276:immunology; D010716:Phosphatidylinositols / Q000096:biosynthesis; D010766:Phosphorylation; D015398:Signal Transduction / Q000276:immunology*; D014443:Tyrosine / Q000378:metabolism
D000911:Antibodies, Monoclonal; D018821:CD18 Antigens; D015848:Interleukin-5; D008024:Ligands; D016177:Macrophage-1 Antigen; D010716:Phosphatidylinositols; D014443:Tyrosine