title: Sensitivity and specificity of an improved rapid latex agglutination test for identification of methicillin-sensitive and -resistant Staphylococcus aureus isolates.
abstract: The performance of a second-generation rapid agglutination kit, Slidex Staph Plus (SSP; bioMérieux), was compared to those of the Slidex Staph (SS; bioMérieux), Staphaurex (SRX; Murex Diagnostics), and BBL Staphyloslide (BBL; Becton Dickinson) kits by using 508 clinical isolates composed of 150 methicillin-sensitive Staphylococcus aureus (MSSA) organisms, 154 methicillin-resistant S. aureus (MRSA) organisms, and 204 non-S. aureus Staphylococcus spp. Of the 508 isolates tested, 75% were fresh clinical isolates, with the remainder taken from five different freezer collections. All four agglutination tests had comparable sensitivities for MSSA and MRSA. However, the SS kit was significantly less specific (93.1%) than the three other tests (P > 0.05, McNemar test). These results demonstrate that the new rapid latex agglutination kit, SSP, was more specific for the identification of S. aureus than the previous version and performed comparably to the SRX and BBL kits.
authors.affiliation: Department of Medicine, Boston University School of Medicine, Boston Veterans Affairs Medical Center, Massachusetts 02130, USA.
authors.forename: S C
authors.initials: SC
authors.lastname: Smole
authors.forename: E
authors.initials: E
authors.lastname: Aronson
authors.forename: A
authors.initials: A
authors.lastname: Durbin
authors.forename: S M
authors.initials: SM
authors.lastname: Brecher
authors.forename: R D
authors.initials: RD
authors.lastname: Arbeit
mesh_terms: D004269:DNA, Bacterial / Q000032:analysis; D007841:Latex Fixation Tests; D016106:Methicillin Resistance; D009693:Nucleic Acid Hybridization; D011933:Reagent Kits, Diagnostic; D012680:Sensitivity and Specificity; D013211:Staphylococcus aureus / Q000187:drug effects / Q000302:isolation & purification*
chemical_list: D004269:DNA, Bacterial; D011933:Reagent Kits, Diagnostic