title: Energetics of isolated hepatocyte swelling induced by sodium co-transported amino acids.
abstract: This study was designed to investigate the energetics of isolated rat hepatocyte swelling due to sodium-cotransported amino acid accumulation in a medium containing either glucose or octanoate as basal substrate. We show that the size of the increase in cytosolic volume is directly correlated with the total amino acid accumulation, which depends on the difference of electrical potential across the plasma membrane. Such a change in cell volume, with either glucose or octanoate, does not modify the mitochondrial volume. Addition of sodium-cotransported amino acids for which the metabolism was avoided showed that the rise in cell volume, per se, did not change the respiratory rate, deltap, or phosphate potential in either mitochondrial or cytosolic compartments. Conversely, the large increase in oxidative phosphorylation flux was due to the metabolism of amino acids as a consequence of a rise in electron supply for the respiratory chain rather than an increase in cellular ATP demand, as indicated by the increase in cytosolic phosphate potential. Moreover, although we confirm that octanoate addition largely increases the respiratory rate by a process different from uncoupling, we observed that the same overall thermodynamic driving force through the respiratory chain and the same mitochondrial or cytosolic phosphate potential were maintained for much higher oxygen consumption when octanoate was present. We propose that these octanoate effects are due to a decrease in the actual protons/2 electrons stoichiometry as a consequence of a shift in electron supply toward a two-coupling site instead of a three-coupling site. The change in the FADH2/NADH formation flux ratio in either fatty acid or carbohydrate oxidation explains such results.
authors.affiliation: Institut de Biochimie et Génétique Cellulaires du CNRS, Bordeaux, France.
authors.forename: P
authors.initials: P
authors.lastname: Espié
authors.forename: A
authors.initials: A
authors.lastname: Devin
authors.forename: B
authors.initials: B
authors.lastname: Guérin
authors.forename: M
authors.initials: M
authors.lastname: Rigoulet
mesh_terms: D000596:Amino Acids / Q000494:pharmacology*; D000818:Animals; D002478:Cells, Cultured; D004734:Energy Metabolism / Q000187:drug effects*; D008099:Liver / Q000166:cytology / Q000502:physiology*; D008297:Male; D051381:Rats; D017208:Rats, Wistar; D012964:Sodium / Q000494:pharmacology*
chemical_list: D000596:Amino Acids; D012964:Sodium