Oxidation of individual fatty acids yields different profiles of oxidation markers.
Free radicals attack lipid molecules and initiate a chain of reactions that may trigger several diseases such as atherosclerosis and cancer. Several markers of oxidative stress have been proposed, including measurements of lipid peroxides and short-chain aldehydes levels and evaluation of conjugated diene formation. Although it is generally assumed that fatty acid oxidizability is directly proportional to their degree of unsaturation, little is known about the contribution of individual fatty acids to each of these markers. We investigated such contributions in a model of AAPH-mediated peroxidation of individual fatty acid micelles by assessing several indices of oxidative stress. The results suggest that the generation of oxidation products by individual fatty acids is not directly related to their degree of unsaturation and indicate that the differential contribution of individual fatty acids should be taken into account and more than one marker of lipid peroxidation should be included in in vivo studies of oxidative stress.
Visioli
F
F
Institute of Pharmacological Sciences, University of Milan, Italy. francesco.visioli@unimi.it
Colombo
C
C
Galli
C
C
D000578:Amidines / Q000494:pharmacology; D015415:Biomarkers / Q000737:chemistry*; D005227:Fatty Acids / Q000737:chemistry*; D005231:Fatty Acids, Unsaturated / Q000737:chemistry; D005609:Free Radicals / Q000378:metabolism; D015227:Lipid Peroxidation / Q000502:physiology; D008054:Lipid Peroxides / Q000032:analysis; D010084:Oxidation-Reduction; D018384:Oxidative Stress / Q000502:physiology*; D017392:Thiobarbituric Acid Reactive Substances / Q000032:analysis
D000578:Amidines; D015415:Biomarkers; D005227:Fatty Acids; D005231:Fatty Acids, Unsaturated; D005609:Free Radicals; D008054:Lipid Peroxides; D017392:Thiobarbituric Acid Reactive Substances; C046728:2,2'-azobis(2-amidinopropane)