title: Cloning of genes specifically expressed in rice embryogenic cells.
abstract: We have examined differences in gene expression pattern between embryogenic callus (EC) and nonembryogenic callus (NEC) derived from mature seed embryo of rice (Oryza sativa L. cv Donggin). Three EC-specific transcripts were identified by differential display of amplified cDNAs. Specific expression of two partial cDNAs, designated as REC1 and REC2, respectively, was confirmed by a northern blot analysis. Partial nucleotide sequence of the clone REC1 showed no homology with any known genes, but partial amino acid sequence deduced from the clone REC2 exhibited 55-82% homology with nickel-cobalt-resistant proteins identified from a bacterium, Alcaligenes eutrophus CH34.
authors.affiliation: Department of Medical Laboratory Technology, Seokang Junior College, Kwangju, Korea.
authors.forename: B K
authors.initials: BK
authors.lastname: Jung
authors.forename: J H
authors.initials: JH
authors.lastname: Pyo
authors.forename: W S
authors.initials: WS
authors.lastname: Kim
authors.forename: B H
authors.initials: BH
authors.lastname: Nam
authors.forename: S J
authors.initials: SJ
authors.lastname: Hwang
authors.forename: B
authors.initials: B
authors.lastname: Hwang
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D003001:Cloning, Molecular; D018507:Gene Expression Regulation, Developmental; D018506:Gene Expression Regulation, Plant; D017343:Genes, Plant / Q000235:genetics*; D008855:Microscopy, Electron, Scanning; D008969:Molecular Sequence Data; D012275:Oryza / Q000196:embryology* / Q000235:genetics* / Q000648:ultrastructure; D016133:Polymerase Chain Reaction; D012639:Seeds / Q000196:embryology* / Q000235:genetics* / Q000648:ultrastructure