Induction of fructose 1,6-bisphosphatase in HL-60 leukemia cells by retinoic acid.
The expression of the fructose 1,6-bisphosphatase gene in HL-60 cells was induced by retinoic acid. The levels of mRNA, enzyme activity and enzyme protein in the cell line began to rapidly increase after culturing with retinoic acid for 72 h. Retinoic acid dose-dependently increased the enzyme activity with maximal stimulation at 1 microM. The responses of the fructose 1,6-bisphosphatase gene expression by retinoic acid were markedly slower than those of the enzyme expression by 1alpha,25-dihydroxyvitamin D3. When HL-60 cells were cultured in the presence of both retinoic acid and 1alpha,25-dihydroxyvitamin D3, the effects of the two agents on enzyme activity, protein and mRNA were additive.
Mizunuma
H
H
Akita University College of Allied Medical Science, Japan.
Tashima
Y
Y
D015153:Blotting, Western; D002117:Calcitriol / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D006597:Fructose-Bisphosphatase / Q000187:drug effects / Q000235:genetics* / Q000378:metabolism*; D018922:HL-60 Cells / Q000187:drug effects / Q000201:enzymology*; D006801:Humans; D012333:RNA, Messenger; D014212:Tretinoin / Q000494:pharmacology*
D012333:RNA, Messenger; D014212:Tretinoin; D006597:Fructose-Bisphosphatase; D002117:Calcitriol