title: Quantitative analysis of polychlorinated biphenyls, organochlorine insecticides, polycyclic aromatic hydrocarbons, polychlorinated hydrocarbons and polynitrohydrocarbons in spiked samples of soil, water and plasma by selected-ion monitoring gas chromatography-mass spectrometry.
abstract: A broad range of pollutants such as polycyclic aromatic hydrocarbons (PAHs), polychlorinated hydrocarbons (PCHs), polynitrohydrocarbons (PNHs), polychlorinated biphenyls (PCBs) and organochlorine (OCs) insecticides were simultaneously analyzed in spiked soil, water or plasma samples by using gas chromatography-mass spectrometry (GC-MS). Water and plasma samples containing the pollutants were extracted by a solid-phase extraction (SPE) method using florisil columns. The soil samples, fortified with the toxicants, were extracted with water, methanol or dichloromethane (DCM). The water extract was processed by the SPE method. The methanol and DCM samples were dried, dissolved in acetonitrile and subjected to the SPE extraction. The extracted samples were analyzed by GC-MS programmed to monitor selected ions. The deuterium labelled compounds were used as the internal standards. The chromatographic profile of total ions indicated complete separation of some compounds such as isophorone, naphthalene, all PCBs, most OC insecticides and PNHs; high Mr PAHs and some PCHs were partially or incompletely separated. The chromatographic profile of individual ion indicated good separation of each ion. The minimum detection limit ranged from 1 to 4 pg injected when 1 or 2 ions were monitored or from 20 to 200 pg injected when 20 ions were monitored. The SPE method that provided 60-105% recovery of pollutants from water samples, provided only 2-60% recovery from plasma samples. This may be due to the binding of pollutants to plasma proteins. Water recovered 1-30%, while methanol or DCM recovered 65-100% of the pollutants added to the soil samples. The use of internal standards corrected for the loss of pollutants from plasma or soil.
authors.affiliation: Department of Veterinary Diagnostic Medicine, College of Veterinary Medicine, University of Minnesota, St. Paul 55108, USA.
authors.forename: A K
authors.initials: AK
authors.lastname: Singh
authors.forename: D
authors.initials: D
authors.lastname: Spassova
authors.forename: T
authors.initials: T
authors.lastname: White
mesh_terms: D004785:Environmental Pollutants / Q000032:analysis* / Q000097:blood; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods*; D006838:Hydrocarbons / Q000032:analysis* / Q000097:blood / Q000737:chemistry; D006843:Hydrocarbons, Chlorinated / Q000032:analysis; D007306:Insecticides / Q000032:analysis* / Q000097:blood / Q000737:chemistry; D011078:Polychlorinated Biphenyls / Q000032:analysis* / Q000097:blood / Q000737:chemistry; D011084:Polycyclic Aromatic Hydrocarbons / Q000032:analysis; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D012989:Soil Pollutants / Q000032:analysis; D014874:Water Pollutants, Chemical / Q000032:analysis
chemical_list: D004785:Environmental Pollutants; D006838:Hydrocarbons; D006843:Hydrocarbons, Chlorinated; D007306:Insecticides; D011084:Polycyclic Aromatic Hydrocarbons; D012989:Soil Pollutants; D014874:Water Pollutants, Chemical; D011078:Polychlorinated Biphenyls