The use of pulsed-field gel electrophoresis for subdivision of Salmonella typhimurium in an outbreak situation.
An institutional outbreak of gastroenteritis provided an opportunity to investigate further the isolates of Salmonella typhimurium by pulsed-field gel electrophoresis (PFGE). Three phage types were identified. Antibiograms identified two types and two distinct patterns were found on PFGE. If phage typing alone is used for epidemiological study of strains, it is possible that an association between strains may be missed.
Corbett-Feeney
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Department of Medical Microbiology, University College Hospital and University College, Galway, Ireland.
Riain
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D000293:Adolescent; D015373:Bacterial Typing Techniques; D001434:Bacteriophage Typing; D004269:DNA, Bacterial / Q000032:analysis; D004196:Disease Outbreaks; D016521:Electrophoresis, Gel, Pulsed-Field; D006801:Humans; D007494:Ireland / Q000453:epidemiology; D008826:Microbial Sensitivity Tests; D012478:Salmonella Food Poisoning / Q000453:epidemiology* / Q000382:microbiology*; D012486:Salmonella typhimurium / Q000145:classification* / Q000302:isolation & purification
D004269:DNA, Bacterial