title: Fractionator analysis shows loss of neurons in the lateral geniculate nucleus of macaques infected with neurovirulent simian immunodeficiency virus.
abstract: Infection of macaques with neurovirulent strains of simian immunodeficiency virus (SIVmac) is an experimental model for the neurological manifestations of AIDS. Loss of neurons has been reported in the cerebral cortex following immunodeficiency viral infection, but thalamic structures which may contribute to electrophysiological changes and neurological deficits have not been examined. In this study, the lateral geniculate nucleus (LGN) of macaques inoculated with macrophage-tropic, neurovirulent virus SIVmac239 (R71 and 17E) was examined for neuron loss using the optical fractionator method. Estimates of the number of neurons in the P layers of the lateral geniculate nucleus of age-matched control macaques ranged from 1.0 to 1.3 x 10(6), while the number of neurons in SIV infected macaques ranged from 0.8 to 1.1 x 10(6), reflecting neuron loss of up to 28%. Neuron loss was not observed in the magnocellular layer. The total number of glia and glial density were unchanged. Loss of neurons in the lateral geniculate nucleus was correlated with the pattern of neuropathological changes. Neuron loss was most severe in animals with encephalitis concentrated in the brain stem and subcortical white matter and was less apparent in animals with diffuse encephalitis. Neuron loss in the lateral geniculate nucleus did not explain changes observed in the visual evoked potential, which was severely affected in two animals which showed a loss of 24 and 26%, while it was normal in a third animal which showed neuron loss of 28%.
authors.affiliation: Department of Anatomy and cell Biology, University of Kansas Medical Center, Kansas City 66160-7400, USA.
authors.forename: N E
authors.initials: NE
authors.lastname: Berman
authors.forename: L A
authors.initials: LA
authors.lastname: Raymond
authors.forename: K A
authors.initials: KA
authors.lastname: Warren
authors.forename: R
authors.initials: R
authors.lastname: Raghavan
authors.forename: S V
authors.initials: SV
authors.lastname: Joag
authors.forename: O
authors.initials: O
authors.lastname: Narayan
authors.forename: P D
authors.initials: PD
authors.lastname: Cheney
mesh_terms: D000818:Animals; D002452:Cell Count; D005829:Geniculate Bodies / Q000473:pathology*; D007150:Immunohistochemistry; D008253:Macaca mulatta; D008297:Male; D009474:Neurons / Q000473:pathology* / Q000821:virology; D009887:Ophthalmoscopy; D010784:Photomicrography; D012160:Retina / Q000473:pathology; D016097:Simian Acquired Immunodeficiency Syndrome / Q000473:pathology* / Q000821:virology; D015302:Simian Immunodeficiency Virus / Q000472:pathogenicity*; D014774:Virulence