The nucleotide and predicted amino acid sequence of the fusion protein of recent isolates of canine distemper virus in Japan.
Analysis of the molecular properties of fusion (F) proteins of field isolates of canine distemper virus (CDV) by immunoprecipitation analysis revealed an identical molecular mass of F protein of 3 field isolates as well as the Onderstepoort laboratory strain. Sequencing showed that the F gene of a field isolate (the Yanaka strain) shared 90.1% and 95.7% identities with the Onderstepoort strain at nucleotide and amino acid levels, respectively. All of the 13 cysteine residues and 4 potential asparagine-linked glycosylation sites were completely conserved amongst these strains. These results indicate that the F proteins is much less heterogeneous than that observed in the hemagglutinin proteins of CDV.
Iwatsuki
K
K
Department of Veterinary Microbiology, Faculty of Agriculture, University of Tokyo, Japan.
Miyashita
N
N
Yoshida
E
E
Shin
Y S
YS
Ohashi
K
K
Kai
C
C
Mikami
T
T
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D017931:DNA Primers; D004217:Distemper Virus, Canine / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification; D004285:Dogs; D008969:Molecular Sequence Data; D010802:Phylogeny; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid; D012689:Sequence Homology, Nucleic Acid; D014760:Viral Fusion Proteins / Q000096:biosynthesis / Q000737:chemistry* / Q000235:genetics*
D017931:DNA Primers; D014760:Viral Fusion Proteins