Use of polymerase chain reaction in the diagnosis of toxocariasis: an experimental study.
In this paper we report the usefulness of polymerase chain reaction technique in the diagnosis of visceral larva migrans in a mouse model. Liver samples obtained from two set of experimentally infected mice (10, 100, 1,000 and 10,000 embryonated Toxocara canis eggs per mouse) along with the eggs of T. canis, T. cati and Ascaris suum were included in this study. Polymerase chain reaction (PCR) was performed using Toxocara primers (SB12). The first PCR product electrophoresis revealed very thin positive bands or no bands in liver samples. However, on second PCR a clear-cut bands were observed. No positive band was shown by A. suum eggs. Our findings thus indicate the usefulness of PCR technic in the diagnosis of visceral larva migrans (VLM) in liver biopsy materials specifically by means of double PCR using the primer SB12.
Rai
S K
SK
Department of Medical Zoology, Kobe University School of Medicine, Japan.
Uga
S
S
Wu
Z
Z
Takahashi
Y
Y
Matsumura
T
T
D000818:Animals; D001706:Biopsy; D018509:DNA, Helminth / Q000032:analysis*; D004195:Disease Models, Animal; D004587:Electrophoresis, Agar Gel; D005260:Female; D007816:Larva Migrans, Visceral / Q000175:diagnosis* / Q000469:parasitology; D051379:Mice; D008813:Mice, Inbred ICR; D016133:Polymerase Chain Reaction / Q000379:methods; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
D018509:DNA, Helminth