Croton ruizianus: platelet proaggregating activity of two new pregnane glycosides.
The MeOH extract of the aerial parts of Croton ruizianus afforded two new pregnane glycosides 1 and 2, together with the morphinandienone alkaloids flavinantine (3) and O-methylflavinantine (4). Their structures were elucidated by NMR experiments including 1H-1H (1D TOCSY and 2D DQF-COSY) and 1H-13C (HSQC, HMBC) spectroscopy. The proaggregating activity of the MeOH extract and the isolates were evaluated. Although the MeOH extract and pregnane glycosides (at different doses) were found to promote platelet aggregation, flavinantine (3) and O-methylflavinantine (4) showed only slight activity. The ability of the MeOH extract and the four compounds to act synergistically with thrombin was also evaluated. All the tested compounds were successful in augmenting the aggregating effect of thrombin, although to different degrees.
Piacente
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Facultad de Farmacia y Bioquimica, Universidad Inca Garcilaso de la Vega, Lima, Pueblo Libre, Peru.
Belisario
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MA
Del Castillo
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H
Pizza
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C
De Feo
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D001792:Blood Platelets / Q000187:drug effects / Q000201:enzymology; D001938:Brazil; D002240:Carbohydrate Sequence; D002470:Cell Survival / Q000187:drug effects; D006027:Glycosides / Q000737:chemistry / Q000494:pharmacology; D006801:Humans; D066298:In Vitro Techniques; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D009682:Magnetic Resonance Spectroscopy; D008969:Molecular Sequence Data; D010946:Plants, Medicinal / Q000737:chemistry*; D010974:Platelet Aggregation / Q000187:drug effects*; D011278:Pregnanes / Q000737:chemistry / Q000494:pharmacology*; D016339:Spectrometry, Mass, Fast Atom Bombardment
D006027:Glycosides; D011278:Pregnanes; D007770:L-Lactate Dehydrogenase