A sequence highly similar to PNG is located on chromosome 22q12 in intron 15 of the LIMK-2 gene.
In this report we describe a sequence (PNG22) highly similar to the Phospholipase C beta3 Neighboring Gene (PNG). We also report that PNG22 is located in the q12 region on chromosome 22 between markers D22S1144 and D22S280. This finding explains that PNG probes cross hybridize to sequences on chromosome 22. Fine mapping using our sequence data and the complete sequence of a PAC clone (DJ515N1), located in this region, determined that PNG22 is located in intron 15 of the LIMK-2 gene. PNG22 is 93% homologous to PNG, however it do not have the introns described for the PNG gene, instead matching the cDNA sequence. This leads us to suggest that PNG22 probably represents a PNG pseudogene. In this report we also list the exon intron borders and the genomic structure of LIMK-2 and place it on the Sanger Center chromosome 22 Physical map. It also explains the finding that PNG probes cross hybridize to sequences on chromosome 22.
Kedra
D
D
Department of Molecular Medicine, Karolinska Hospital, Stockholm, Sweden.
Carson
E
E
Weber
G
G
Lagercrantz
J
J
D001483:Base Sequence; D002892:Chromosomes, Human, Pair 22; D003001:Cloning, Molecular; D004268:DNA-Binding Proteins; D006801:Humans; D007438:Introns; D054461:Lim Kinases; D008969:Molecular Sequence Data; D011494:Protein Kinases / Q000235:genetics*; D054645:Protein Phosphatase 1; D017346:Protein Serine-Threonine Kinases; D011506:Proteins / Q000235:genetics*; D011544:Pseudogenes; D012689:Sequence Homology, Nucleic Acid
D004268:DNA-Binding Proteins; C099933:Ppp1r14b protein, mouse; D011506:Proteins; D011494:Protein Kinases; C102895:LIMK2 protein, human; D054461:Lim Kinases; D017346:Protein Serine-Threonine Kinases; D054645:Protein Phosphatase 1