title: Replication checkpoint enforced by kinases Cds1 and Chk1.
abstract: Cdc2, the kinase that induces mitosis, is regulated by checkpoints that couple mitosis to the completion of DNA replication and repair. The repair checkpoint kinase Chk1 regulates Cdc25, a phosphatase that activates Cdc2. Effectors of the replication checkpoint evoked by hydroxyurea (HU) are unknown. Treatment of fission yeast with HU stimulated the kinase Cds1, which appears to phosphorylate the kinase Wee1, an inhibitor of Cdc2. The protein kinase Cds1 was also required for a large HU-induced increase in the amount of Mik1, a second inhibitor of Cdc2. HU-induced arrest of cell division was abolished in cds1 chk1 cells. Thus, Cds1 and Chk1 appear to jointly enforce the replication checkpoint.
authors.affiliation: Departments of Molecular Biology and Cell Biology, Scripps Research Institute, La Jolla, CA 92037, USA.
authors.forename: M N
authors.initials: MN
authors.lastname: Boddy
authors.forename: B
authors.initials: B
authors.lastname: Furnari
authors.forename: O
authors.initials: O
authors.lastname: Mondesert
authors.forename: P
authors.initials: P
authors.lastname: Russell
mesh_terms: D016203:CDC2 Protein Kinase / Q000037:antagonists & inhibitors / Q000378:metabolism; D002453:Cell Cycle; D018797:Cell Cycle Proteins / Q000378:metabolism; D000071877:Checkpoint Kinase 1; D064447:Checkpoint Kinase 2; D004260:DNA Repair; D004261:DNA Replication / Q000187:drug effects; D006918:Hydroxyurea / Q000494:pharmacology; D009687:Nuclear Proteins; D010749:Phosphoprotein Phosphatases / Q000378:metabolism; D010766:Phosphorylation; D011494:Protein Kinases / Q000378:metabolism*; D017346:Protein Serine-Threonine Kinases; D011505:Protein-Tyrosine Kinases / Q000378:metabolism; D011993:Recombinant Fusion Proteins / Q000378:metabolism; D016196:S Phase; D012568:Schizosaccharomyces / Q000166:cytology / Q000201:enzymology / Q000378:metabolism*; D029702:Schizosaccharomyces pombe Proteins; D020687:cdc25 Phosphatases
chemical_list: D018797:Cell Cycle Proteins; D009687:Nuclear Proteins; D011993:Recombinant Fusion Proteins; D029702:Schizosaccharomyces pombe Proteins; D011494:Protein Kinases; C090679:mik1 protein, S pombe; C068689:wee1 protein, S pombe; D064447:Checkpoint Kinase 2; D011505:Protein-Tyrosine Kinases; C578636:Cds1 protein, S pombe; D000071877:Checkpoint Kinase 1; C000605873:Chk1 protein, S pombe; D017346:Protein Serine-Threonine Kinases; D016203:CDC2 Protein Kinase; D010749:Phosphoprotein Phosphatases; D020687:cdc25 Phosphatases; D006918:Hydroxyurea