title: Mathematical modeling and fluorescence imaging to study the Ca2+ turnover in skinned muscle fibers.
abstract: A mathematical model was developed for the simulation of the spatial and temporal time course of Ca2+ ion movement in caffeine-induced calcium transients of chemically skinned muscle fiber preparations. Our model assumes cylindrical symmetry and quantifies the radial profile of Ca2+ ion concentration by solving the diffusion equations for Ca2+ ions and various mobile buffers, and the rate equations for Ca2+ buffering (mobile and immobile buffers) and for the release and reuptake of Ca2+ ions by the sarcoplasmic reticulum (SR), with a finite-difference algorithm. The results of the model are compared with caffeine-induced spatial Ca2+ transients obtained from saponin skinned murine fast-twitch fibers by fluorescence photometry and imaging measurements using the ratiometric dye Fura-2. The combination of mathematical modeling and digital image analysis provides a tool for the quantitative description of the total Ca2+ turnover and the different contributions of all interacting processes to the overall Ca2+ transient in skinned muscle fibers. It should thereby strongly improve the usage of skinned fibers as quantitative assay systems for many parameters of the SR and the contractile apparatus helping also to bridge the gap to the intact muscle fiber.
authors.affiliation: Ruprecht-Karls-Universität Heidelberg, II Institute of Physiology, Germany.
authors.forename: D
authors.initials: D
authors.lastname: Uttenweiler
authors.forename: C
authors.initials: C
authors.lastname: Weber
authors.forename: R H
authors.initials: RH
authors.lastname: Fink
mesh_terms: D000818:Animals; D055592:Biophysical Phenomena; D001703:Biophysics; D002110:Caffeine / Q000494:pharmacology; D002118:Calcium / Q000378:metabolism*; D005456:Fluorescent Dyes; D016257:Fura-2; D066298:In Vitro Techniques; D017136:Ion Transport / Q000187:drug effects; D007700:Kinetics; D008297:Male; D008433:Mathematics; D051379:Mice; D008807:Mice, Inbred BALB C; D008954:Models, Biological; D018656:Muscle Fibers, Fast-Twitch / Q000187:drug effects / Q000378:metabolism*; D012519:Sarcoplasmic Reticulum / Q000378:metabolism
chemical_list: D005456:Fluorescent Dyes; D002110:Caffeine; D002118:Calcium; D016257:Fura-2