title: Long-term survival of Streptococcus pneumoniae at room temperature on Dorset egg medium.
abstract: Forty-five isolates of Streptococcus pneumoniae were inoculated on Dorset egg and supplemented Columbia agar base media, incubated overnight at 37 degrees C, and then kept at room temperature (RT; 21 degrees C) or 4 degrees C. Long-term viability was best at RT for both media, with all isolates remaining viable on Dorset egg medium for 44 days; viability was 90 and 57% on Columbia agar base medium after 7 and 30 days. We recommend the use of Dorset egg medium for the maintenance of pneumococci at RT.
authors.affiliation: MRC/SAIMR/WITS Pneumococcal Diseases Research Unit, South African Institute for Medical Research, Hillbrow, Johannesburg.
authors.forename: A D
authors.initials: AD
authors.lastname: Wasas
authors.forename: R E
authors.initials: RE
authors.lastname: Huebner
authors.forename: M
authors.initials: M
authors.lastname: De Blanche
authors.forename: K P
authors.initials: KP
authors.lastname: Klugman
mesh_terms: D003470:Culture Media; D013296:Streptococcus pneumoniae / Q000502:physiology*; D013696:Temperature
chemical_list: D003470:Culture Media