Binding of monomeric and oligomeric porphyrins to human glioblastoma (U-87MG) cells and their photosensitivity.
The binding of monomeric (Hp) and oligomeric (PHE) forms of porphyrin to glioblastoma (U-87MG) cells and the photosensitization of these cells have been studied. Upon binding to U-87MG cells, Hp and PHE exhibited fluorescence bands at 615 and 636 nm, respectively. The fluorescence and absorption spectra of Hp, HpD and PHE, measured in different solvents, suggest that the 615 nm band may arise due to the binding of monomeric as well as aggregated forms of porphyrin to the hydrophilic sites in the cells whereas the 636 nm band may be due to the binding of an aggregated form of porphyrin to the hydrophobic sites. The photosensitivity of cells and photo-induced lipid peroxidation were measured as a function of light dose. Cells were found most photosensitive to PHE followed by HpD and Hp. The photosensitivity of cells correlates well with the fluorescence intensity of cell bound dye at 636 nm. These results suggest that the binding of the oligomeric component of HpD to hydrophobic sites in the cells is responsible for the enhancement in the photosensitivity.
Sreenivasan
R
R
Department of Biophysics, National Institute of Mental Health and Neurosciences, Bangalore, India.
Muruganandham
M
M
Sharma
M
M
Joshi
P G
PG
Joshi
N B
NB
D001932:Brain Neoplasms / Q000188:drug therapy / Q000378:metabolism*; D017323:Dihematoporphyrin Ether / Q000378:metabolism*; D005909:Glioblastoma / Q000188:drug therapy / Q000378:metabolism*; D017324:Hematoporphyrin Derivative / Q000378:metabolism*; D006801:Humans; D010778:Photochemotherapy; D017319:Photosensitizing Agents / Q000378:metabolism*; D013050:Spectrometry, Fluorescence; D013057:Spectrum Analysis; D014407:Tumor Cells, Cultured
D017319:Photosensitizing Agents; D017324:Hematoporphyrin Derivative; D017323:Dihematoporphyrin Ether