Cytologic application of p53 overexpression using immunocytochemistry and confocal laser scanning microscopy.
OBJECTIVE
p53 Protein plays an important role in cellular growth control. This study investigated p53 protein expression in cell smears of endometrial carcinomas supplemented by confocal laser scanning microscopy (CLSM).


METHODS
Imprints from surgical specimens of 20 endometrial carcinomas were used. p53 Protein expression was investigated immunocytochemically using the monoclonal antibody pAb1801. Using CLSM, three-dimensional morphology was studied.


RESULTS
Of the 20 cases of endometrial carcinoma, 8 stained positively for p53 protein. p53 Showed heterogeneous intranuclear localization, which appeared to be associated with chromatin structure.


CONCLUSIONS
Immunocytochemical detection of p53 overexpression in cell samples is practical, and CLSM has vast potentials in studying the intranuclear arrangement of chromatin.
Sato
S
S
Department of Obstetrics and Gynecology, Tohoku University School of Medicine, Sendai, Japan.
Ito
K
K
Jiko
K
K
Konno
R
R
Yajima
A
A
Sasano
H
H
D000818:Animals; D003584:Cytological Techniques; D016889:Endometrial Neoplasms / Q000378:metabolism* / Q000473:pathology*; D005260:Female; D019084:Fluorescent Antibody Technique, Indirect; D006801:Humans; D018613:Microscopy, Confocal; D011817:Rabbits; D016159:Tumor Suppressor Protein p53 / Q000032:analysis*
D016159:Tumor Suppressor Protein p53