The malignant capacity of skin tumours induced by expression of a mutant H-ras transgene depends on the cell type targeted.
BACKGROUND
. Pinpointing the cells from which tumours arise is a major challenge n tumour biology. Previous work has shown that the targeted expression of a mutant ras gene within the interfollicular cell compartment of mouse skin induces the formation of benign papillomas, but these do not spontaneously progress to malignancy. We have investigated the carcinogenic effects of expressing the same oncogene in a different population of epidermal cells.


RESULTS
Expression of mutant ras from a truncated keratin 5 gene promoter, which directs expression to the follicular and interfollicular cells of newborn mice and the hair follicle cells of adults, stimulated the development of acanthotic areas in newborn mice. Within one week of birth, the acanthotic skin developed areas of carcinoma in situ and adult mice developed papillomas and keratoacanthomas, the latter having a high frequency of spontaneous malignant transformation to squamous and occasionally spindle carcinomas. The benign tumours that arose had several hallmarks of tumours at a high risk of malignant progression, including suprabasal cell proliferation and heterogeneous expression of keratin 13. In contrast to tumours induced by expressing mutant ras under the control of the keratin 10 or keratin 1 gene promoters, the formation of these lesions was not dependent on wounding or a tumour promoter.


CONCLUSIONS
Benign tumours that are at a risk of malignant conversion are primarily derived from cells located within the hair follicle, and the nature of the cell in which tumour initiation occurs is a major determinant of malignant potential.
Brown
K
K
CRC Beatson Laboratories Department of Medical Oncology Alexander Stone Building University of Glasgow Garscube Estate, Switchback Road, Bearsden, Glasgow, G61 1BD, UK.
Strathdee
D
D
Bryson
S
S
Lambie
W
W
Balmain
A
A
D000818:Animals; D000831:Animals, Newborn; D015972:Gene Expression Regulation, Neoplastic / Q000235:genetics*; D011905:Genes, ras / Q000235:genetics*; D018859:Hair Follicle / Q000166:cytology / Q000503:physiopathology; D007633:Keratins / Q000235:genetics; D007763:Lac Operon / Q000235:genetics; D051379:Mice; D008822:Mice, Transgenic; D009154:Mutation / Q000235:genetics*; D011401:Promoter Regions, Genetic / Q000235:genetics / Q000502:physiology; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis / Q000235:genetics; D012868:Skin Abnormalities / Q000473:pathology; D012878:Skin Neoplasms / Q000235:genetics* / Q000503:physiopathology*
D011993:Recombinant Fusion Proteins; D007633:Keratins