Induction of pulmonary allergic responses by antigen-specific Th2 cells.
The development of pulmonary allergic responses was examined in mice following pulmonary transfer of Ag (conalbumin)-specific Th2 cells. The levels of serum-specific IgE, cellular infiltrates, airway mucus goblet cells, and airway responsiveness were analyzed and compared with those in Ag-sensitized and -challenged mice. Pulmonary transfer of the conalbumin-specific Th2 clone (D10) induced, in an Ag-specific manner, high levels of the Th2 cytokines IL-4 and IL-5 in the bronchoalveolar lavage fluids and mucosal eosinophils, concomitant with an increase in airway responsiveness. The D10 cell-induced responses were seen in the absence of serum specific IgE. In the presence of Ag, the transferred D10 cells not only remained in the lungs, but also increased in number 72 h post-cell transfer. Although significantly higher levels of IL-4 and IL-5 in the bronchoalveolar lavage fluids were found in D10-transferred mice, the levels of pulmonary eosinophilia, mucus goblet cells, and airway responsiveness were significantly lower than those in Ag-sensitized and -challenged mice. These results demonstrate that although Ag-specific activation of Th2 cells at mucosal sites is able to mediate the recruitment of eosinophils and the subsequent induction of airway hyper-responsiveness, the more severe pulmonary allergic responses were observed only in mice sensitized and challenged with Ag.
Li
X M
XM
Johns Hopkins Asthma and Allergy Center, School of Medicine, The Johns Hopkins University, Baltimore, MD 21224, USA.
Schofield
B H
BH
Wang
Q F
QF
Kim
K H
KH
Huang
S K
SK
D000280:Administration, Inhalation; D019264:Adoptive Transfer; D000485:Allergens / Q000008:administration & dosage; D000818:Animals; D016535:Bronchial Hyperreactivity / Q000276:immunology; D001992:Bronchoalveolar Lavage Fluid / Q000276:immunology; D002460:Cell Line; D002465:Cell Movement / Q000276:immunology; D003207:Conalbumin / Q000008:administration & dosage / Q000276:immunology; D004804:Eosinophils / Q000473:pathology; D018984:Epitopes, T-Lymphocyte / Q000276:immunology*; D007073:Immunoglobulin E / Q000097:blood; D008168:Lung / Q000737:chemistry / Q000276:immunology* / Q000473:pathology; D008297:Male; D051379:Mice; D008806:Mice, Inbred AKR; D008856:Microscopy, Fluorescence; D011506:Proteins / Q000378:metabolism; D012130:Respiratory Hypersensitivity / Q000276:immunology* / Q000378:metabolism / Q000473:pathology; D018418:Th2 Cells / Q000276:immunology* / Q000637:transplantation
D000485:Allergens; D018984:Epitopes, T-Lymphocyte; D011506:Proteins; D003207:Conalbumin; D007073:Immunoglobulin E