Impaired in vivo tumor growth of human pancreatic carcinoma cells retrovirally transduced with GM-CSF gene.
We have examined the antitumor effect of human pancreatic carcinoma cells (AsPC-1) retrovirally transduced with mouse granulocyte macrophage-colony stimulating factor (GM-CSF) gene in nude mice. Growth retardation of the subcutaneous tumors of GM-CSF-producing AsPC-1 cells was observed, although their in vitro proliferation was not different from that of wild-type cells. Histological examination revealed infiltration of monocytic cells into the tumor of GM-CSF-producing cells, and they were shown to be mainly CD11b positive cells by immunohistochemical staining. The survival of the mice inoculated intraperitoneally with GM-CSF- producing AsPC-1 cells was significantly prolonged compared with that of the mice inoculated with wild-type AsPC-1 cells. Thus, the expression of GM-CSF gene in human pancreatic cells induced an antitumor effect in vivo even in the mature T cell-deficient condition.
Kimura
M
M
Division of Pathology, Chiba Cancer Center Research Institute, Japan.
Tagawa
M
M
Yoshida
Y
Y
Takenouchi
T
T
Takenaga
K
K
Azuma
K
K
Yamaguchi
T
T
Saisho
H
H
Sakiyama
S
S
D000818:Animals; D002455:Cell Division; D016178:Granulocyte-Macrophage Colony-Stimulating Factor / Q000235:genetics*; D006801:Humans; D007111:Immunity, Cellular; D016177:Macrophage-1 Antigen / Q000032:analysis; D051379:Mice; D008819:Mice, Nude; D009368:Neoplasm Transplantation; D010190:Pancreatic Neoplasms / Q000235:genetics / Q000276:immunology / Q000473:pathology*; D016019:Survival Analysis; D014162:Transfection; D014183:Transplantation, Heterologous
D016177:Macrophage-1 Antigen; D016178:Granulocyte-Macrophage Colony-Stimulating Factor