N-glycosylation of a baculovirus-expressed recombinant glycoprotein in three insect cell lines.
The capacity of two Trichoplusia ni (TN-368 and BTI-Tn-5b 1-4) and a Spodoptera frugiperda (IPLB-SF-21A) cell lines to glycosylate recombinant, baculovirus-encoded, secreted, placental alkaline phosphatase was compared. The alkaline phosphatase from serum-containing, cell culture medium was purified by phosphate affinity column chromatography. The N-linked oligosaccharides were released from the purified protein with PNGase F and analyzed by fluorophore-assisted carbohydrate electrophoresis. The majority of oligosaccharide structures produced by the three cell lines contained two or three mannose residues, with and without core fucosylation, but there were structures containing up to seven mannose residues. The oligosaccharides that were qualitatively or quantitatively different between the cell lines were sequenced with glycosidase digestions. The S. frugiperda cells produced more fucosylated oligosaccharides than either of the T. ni cell lines. The smallest oligosaccharide produced by S. frugiperda cells was branched trimannose. In contrast, both T. ni cell lines produced predominantly dimannose and linear trimannose structures devoid of alpha 1-3-linked mannose.
Kulakosky
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PC
Boyce Thompson Institute for Plant Research, Ithaca, New York 14853, USA.
Shuler
M L
ML
Wood
H A
HA
D000469:Alkaline Phosphatase / Q000032:analysis* / Q000235:genetics; D000818:Animals; D002240:Carbohydrate Sequence; D002460:Cell Line; D005822:Genetic Vectors; D006023:Glycoproteins / Q000032:analysis* / Q000235:genetics; D006031:Glycosylation; D008969:Molecular Sequence Data; D009036:Moths; D017924:Nucleopolyhedroviruses; D009844:Oligosaccharides / Q000032:analysis*; D010920:Placenta / Q000201:enzymology*; D011993:Recombinant Fusion Proteins / Q000032:analysis / Q000235:genetics; D018411:Spodoptera
D006023:Glycoproteins; D009844:Oligosaccharides; D011993:Recombinant Fusion Proteins; D000469:Alkaline Phosphatase