Short-term effect of dexamethasone on fatty acid and cholesterol synthesis in isolated rat hepatocytes.
The short-term effect of dexamethasone, a synthetic glucocorticoid, on both fatty acid and cholesterol synthesis has been investigated in rat hepatocyte cultures. Within 4h following hormone addition to the cultures, a noticeable stimulation of labelled acetate incorporation into fatty acids was observed. Similar behaviour was noticed when [3H]H2O was used as an independent index of the lipogenic activity. In the same cultures, however, cholesterol synthesis from both [14C]acetate or [3H]H2O was significantly reduced by dexamethasone addition. In these conditions, no significative variation of cholesterol synthesis starting from labelled mevalonate was observed.
Giudetti
A M
AM
Dept. of Biology, University of Lecce, Italy.
Gnoni
G V
GV
D000085:Acetates / Q000378:metabolism / Q000493:pharmacokinetics; D000818:Animals; D002250:Carbon Radioisotopes; D002478:Cells, Cultured; D002784:Cholesterol / Q000096:biosynthesis*; D003907:Dexamethasone / Q000494:pharmacology*; D005227:Fatty Acids / Q000096:biosynthesis*; D005938:Glucocorticoids / Q000494:pharmacology; D050356:Lipid Metabolism; D008099:Liver / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D008297:Male; D051381:Rats; D017208:Rats, Wistar; D014316:Tritium / Q000378:metabolism / Q000493:pharmacokinetics
D000085:Acetates; D002250:Carbon Radioisotopes; D005227:Fatty Acids; D005938:Glucocorticoids; D014316:Tritium; D003907:Dexamethasone; D002784:Cholesterol