title: Regulation of the glial glutamate transporter GLT-1 by glutamate and delta-opioid receptor stimulation.
abstract: The excitatory effect of presynaptically released glutamate is tightly regulated and terminated by high affinity sodium-dependent glutamate transporters. The regulation of the glial glutamate transporter GLT-1 is potentially important in synaptic modulation. Using astroglial cultures prepared from the rat cerebral cortex, we found that the delta-opioid receptor agonist [D-pen2,D-pen5]-enkephalin decreases and glutamate increases the expression of the GLT-1 transporter mRNA. Corresponding changes in the uptake kinetics were found after incubation for 48 h with the respective agonists when glial glutamate uptake was measured in primary astroglial cultures. The data suggest that long-term receptor activation induces alterations in glial glutamate uptake properties.
authors.affiliation: Department of Neurology, Göteborg University, Sweden.
authors.forename: T
authors.initials: T
authors.lastname: Thorlin
authors.forename: R S
authors.initials: RS
authors.lastname: Roginski
authors.forename: K
authors.initials: K
authors.lastname: Choudhury
authors.forename: M
authors.initials: M
authors.lastname: Nilsson
authors.forename: L
authors.initials: L
authors.lastname: Rönnbäck
authors.forename: E
authors.initials: E
authors.lastname: Hansson
authors.forename: P S
authors.initials: PS
authors.lastname: Eriksson
mesh_terms: D018528:ATP-Binding Cassette Transporters / Q000378:metabolism*; D027322:Amino Acid Transport System X-AG; D000818:Animals; D001692:Biological Transport / Q000502:physiology; D020881:Enkephalin, D-Penicillamine (2,5)-; D004745:Enkephalins / Q000494:pharmacology; D005786:Gene Expression Regulation / Q000235:genetics; D018698:Glutamic Acid / Q000493:pharmacokinetics / Q000494:pharmacology*; D007700:Kinetics; D008856:Microscopy, Fluorescence; D009457:Neuroglia; D018377:Neurotransmitter Agents / Q000494:pharmacology; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D017465:Receptors, Opioid, delta / Q000819:agonists / Q000037:antagonists & inhibitors / Q000378:metabolism*; D009435:Synaptic Transmission / Q000502:physiology
chemical_list: D018528:ATP-Binding Cassette Transporters; D027322:Amino Acid Transport System X-AG; D004745:Enkephalins; D018377:Neurotransmitter Agents; D012333:RNA, Messenger; D017465:Receptors, Opioid, delta; D018698:Glutamic Acid; D020881:Enkephalin, D-Penicillamine (2,5)-