c-Fos deficiency inhibits induction of mRNA for some, but not all, neurotransmitter biosynthetic enzymes by immobilization stress.
Recent studies indicated that c-Fos protein may be mediating stress-elicited transcriptional activation of genes involved in neurotransmitter biosynthesis. However, direct evidence for c-Fos mediating these changes in gene expression has been lacking. Mice with disrupted c-fos gene (+/- or -/- genotypes) were used to examine the effect of immobilization stress on a group of stress-responsive genes. In male adrenals, c-Fos was found not essential for stress-elicited activation of expression of tyrosine hydroxylase, dopamine beta-hydroxylase (DBH), phenylethanolamine N-methyltransferase, or neuropeptide Y. In females, immobilization failed to induce adrenal DBH in the c-Fos-deficient mice. In brainstem, c-Fos was indispensable for elevation of DBH mRNA in both genders. The gene, gender, and tissue specificity in the requirement for c-Fos points to diversity in adaptation mechanisms to stress.
Serova
L I
LI
Department of Biochemistry and Molecular Biology, New York Medical College, Valhalla 10595, USA.
Saez
E
E
Spiegelman
B M
BM
Sabban
E L
EL
D000818:Animals; D004299:Dopamine beta-Hydroxylase / Q000235:genetics; D004790:Enzyme Induction / Q000502:physiology; D004798:Enzymes / Q000235:genetics* / Q000378:metabolism; D005260:Female; D007103:Immobilization; D008297:Male; D051379:Mice; D009478:Neuropeptide Y / Q000378:metabolism; D018377:Neurotransmitter Agents / Q000096:biosynthesis*; D010625:Phenylethanolamine N-Methyltransferase / Q000378:metabolism; D016760:Proto-Oncogene Proteins c-fos / Q000172:deficiency*; D012333:RNA, Messenger / Q000378:metabolism*; D013312:Stress, Physiological / Q000209:etiology / Q000378:metabolism*; D014446:Tyrosine 3-Monooxygenase / Q000378:metabolism
D004798:Enzymes; D009478:Neuropeptide Y; D018377:Neurotransmitter Agents; D016760:Proto-Oncogene Proteins c-fos; D012333:RNA, Messenger; D014446:Tyrosine 3-Monooxygenase; D004299:Dopamine beta-Hydroxylase; D010625:Phenylethanolamine N-Methyltransferase