title: Evidence of the existence of alpha 1B-adrenoceptors on hepatocytes of domestic fowl (Gallus domesticus).
abstract: In liver cell membranes of laying hens alpha 1-adrenergic receptors were characterized using 3H-prazosin. Specific binding was saturable and indicative of a single class of high-affinity binding sites with a KD of 0.40 +/- 16 nM and a Bmax of 390 +/- 38 fmol/mg membrane protein. Preincubation of membranes with chlorethylclonidine significantly diminished the binding of 3H-prazosin (Bmax = 99 +/- 14 fmol/mg membrane protein). Furthermore, competition experiments revealed a rank order of prazosin >> phentolamine = oxymetazoline >> WB 4101, with Ki values of 0.71 +/- 0.62, 20 +/- 6, 32 +/- 13, and 119 +/- 48 nM, respectively. These results suggest the existence of alpha 1B-adrenergic receptors in hen liver cell membranes.
authors.affiliation: Department of Veterinary Biochemistry, Faculty of Veterinary Medicine, University of Leipzig, Germany.
authors.forename: E
authors.initials: E
authors.lastname: Neubert
authors.forename: J
authors.initials: J
authors.lastname: Gottschalk
authors.forename: E
authors.initials: E
authors.lastname: Grün
authors.forename: H
authors.initials: H
authors.lastname: Gürtler
mesh_terms: D000818:Animals; D001667:Binding, Competitive; D002462:Cell Membrane / Q000378:metabolism; D002645:Chickens; D005260:Female; D008099:Liver / Q000378:metabolism*; D010058:Oviposition; D011224:Prazosin / Q000378:metabolism; D018340:Receptors, Adrenergic, alpha-1 / Q000378:metabolism*
chemical_list: D018340:Receptors, Adrenergic, alpha-1; D011224:Prazosin