Isolation and identification of acetyl-CoA carboxylase inhibitors from green tea (Camellia sinensis).
An aqueous methanol extract from green tea showed potent acetyl-CoA carboxylase inhibitory activity. An active compound was isolated from the extract and identified as (-)-epigallocatechin gallate by instrumental analyses. The IC50 value of (-)-epigallocatechin gallate was 3.1 x 10(-4) M. Among tea catechins and related compounds, nearly equal activity was found in (-)-epigallocatechin gallate and (-)-epicatechin gallate, whereas (+)-catechin, (-)-epicatechin, (-)-epigallocatechin, gallic acid and methyl gallate each had no inhibitory activity. These results indicate that the 3-O-gallate group of the catechin structure was necessary for this activity. (-)-Epigallocatechin gallate inhibited triglyceride accumulation in 3T3-L1 cells at a concentration of 1.0 x 10(-7) M or higher.
Watanabe
J
J
Department of Bioscience and Chemistry, Faculty of Agriculture, Hokkaido University, Sapporo, Japan. junk@chem.agr.hokudai.ac.jp
Kawabata
J
J
Niki
R
R
D000103:Acetyl-CoA Carboxylase / Q000037:antagonists & inhibitors*; D000818:Animals; D002851:Chromatography, High Pressure Liquid; D004791:Enzyme Inhibitors / Q000302:isolation & purification* / Q000494:pharmacology*; D050356:Lipid Metabolism; D010936:Plant Extracts / Q000302:isolation & purification* / Q000494:pharmacology*; D051381:Rats; D013329:Structure-Activity Relationship; D013662:Tea / Q000737:chemistry*
D004791:Enzyme Inhibitors; D010936:Plant Extracts; D013662:Tea; D000103:Acetyl-CoA Carboxylase