Laminin inhibits Abeta42 fibril formation in vitro.
In the present study, we investigated whether or not laminin inhibits Abeta42 fibril formation in the same manner as Abeta40. Both a thioflavine-T fluorometric assay and electron microscopy by negative staining demonstrated laminin to have a concentration-dependent inhibitory effect on Abeta42 fibril formation. The amyloid fibril formation was inhibited approximately by 70% due to the presence of 1.0 mg/ml laminin co-incubated with 1. 0 mg/ml Abeta42 peptide (molar ratio; Abeta42 peptide:laminin=200:1). These results thus suggested that laminin or its derivatives may be effective as therapeutic agents to either prevent or slow down the progression of amyloidogenesis in Alzheimer's disease.
Monji
A
A
Department of Neuropsychiatry, Faculty of Medicine, Kyushu University, Fukuoka 812-82, Japan.
Tashiro
K
K
Yoshida
I
I
Tashiro
N
N
D016229:Amyloid beta-Peptides / Q000187:drug effects* / Q000378:metabolism; D000818:Animals; D066298:In Vitro Techniques; D007797:Laminin / Q000494:pharmacology*; D051379:Mice; D008854:Microscopy, Electron; D010446:Peptide Fragments / Q000187:drug effects* / Q000378:metabolism
D016229:Amyloid beta-Peptides; D007797:Laminin; D010446:Peptide Fragments; C078592:amyloid beta-protein (1-40); C075222:amyloid beta-protein (1-42)