title: Expression of membrane-associated proteins by strains of enteroaggregative Escherichia coli.
abstract: Certain strains of enteroaggregative Escherichia coli express an outer membrane-associated protein, involved with the adhesion of these bacteria to HEp-2 cells. Strains of enteroaggregative E. coli hybridising with DNA probes for aggregative adhesion, diffuse adhesion and aggregative adhesion fimbriac II expressed an outer membrane-associated protein of 18 kDa regulated by magnesium ions. Strains hybridising with the aggregative adhesion probe only expressed a 20-kDa outer membrane-associated protein regulated by calcium and magnesium. The present study describes two populations of enteroaggregative E. coli which appear to adhere to HEp-2 cells by expressing antigenically distinct, negatively charged membrane-associated proteins.
authors.affiliation: Laboratory of Enteric Pathogens, Central Public Health Laboratory, London, UK.
authors.forename: J
authors.initials: J
authors.lastname: Spencer
authors.forename: H
authors.initials: H
authors.lastname: Chart
authors.forename: H R
authors.initials: HR
authors.lastname: Smith
authors.forename: B
authors.initials: B
authors.lastname: Rowe
mesh_terms: D000818:Animals; D001422:Bacterial Adhesion; D001425:Bacterial Outer Membrane Proteins / Q000032:analysis / Q000096:biosynthesis*; D015153:Blotting, Western; D002460:Cell Line / Q000382:microbiology; D002462:Cell Membrane / Q000648:ultrastructure; D004269:DNA, Bacterial / Q000235:genetics; D004274:DNA, Recombinant / Q000235:genetics; D004926:Escherichia coli / Q000378:metabolism*; D006384:Hemagglutination / Q000276:immunology; D006801:Humans; D008099:Liver; D011817:Rabbits; D051381:Rats
chemical_list: D001425:Bacterial Outer Membrane Proteins; D004269:DNA, Bacterial; D004274:DNA, Recombinant