IL-4, IL-5 and IL-10 are not required for the control of M. bovis-BCG infection in mice.
Mycobacterial infections in mice are normally characterized by a profound Th1 cell-mediated immune response, in which T cells secrete large amounts of IFN-gamma. Recent evidence suggests that this response also includes a Th2 component. In order to investigate whether production of IL-4, IL-5, or IL-10 influenced the outcome of a Mycobacterium bovis-bacille Calmette-Guérin (BCG) infection, we intranasally infected IL-4, IL-5, and IL-10 gene-deficient and control mice and monitored the resulting immune response and bacterial clearance. IL-4, IL-5, and IL-10 deficient mice cleared the mycobacteria with the same kinetics as control mice. Furthermore, T cells of cytokine deficient and control mice produced similar levels of IFN-gamma following in vitro stimulation with purified protein derivative (PPD) from M. bovis. We conclude that the cytokines IL-4, IL-5 and IL-10 are not essential for and do not negatively influence the protective immune response against M. bovis-BCG in the lung of mice.
Erb
K J
KJ
Malaghan Institute of Medical Research, Wellington, New Zealand.
Kirman
J
J
Delahunt
B
B
Chen
W
W
Le Gros
G
G
D000281:Administration, Intranasal; D000818:Animals; D002455:Cell Division; D004195:Disease Models, Animal; D005260:Female; D017353:Gene Deletion; D007372:Interferons / Q000378:metabolism; D016753:Interleukin-10 / Q000235:genetics / Q000276:immunology*; D015847:Interleukin-4 / Q000235:genetics / Q000276:immunology*; D015848:Interleukin-5 / Q000235:genetics / Q000276:immunology*; D007700:Kinetics; D008198:Lymph Nodes / Q000276:immunology; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D009163:Mycobacterium bovis / Q000276:immunology*; D018418:Th2 Cells / Q000276:immunology; D014373:Tuberculin / Q000276:immunology; D014376:Tuberculosis / Q000276:immunology / Q000517:prevention & control*
D015848:Interleukin-5; D014373:Tuberculin; D016753:Interleukin-10; D015847:Interleukin-4; D007372:Interferons