Is absence of pyruvate dehydrogenase complex in mitochondria a possible explanation of significant aerobic glycolysis by normal human leukocytes?
The oxygen consumption of leukocyte mitochondria of both healthy donors and leukemic patients was tested by using different respiratory substrates. The results indicate that pyruvate could not be utilized by mitochondria of normal leukocytes, whereas mitochondria of leukemic leukocytes could use pyruvate as a good respiratory substrate. A search for the possible presence of pyruvate dehydrogenase complex (PDC) in leukocytes indicates that this enzyme is apparently absent in mitochondria of normal leukocytes but is quite active in mitochondria of leukemic leukocytes. The absence of PDC in normal leukocyte mitochondria can explain the phenomenon of significant aerobic glycolysis that has been observed in normal leukocytes.
Biswas
S
S
Department of Biological Chemistry, Indian Association for the Cultivation of Science, Jadavpur, Calcutta.
Ray
M
M
Misra
S
S
Dutta
D P
DP
Ray
S
S
D000105:Acetyl Coenzyme A / Q000378:metabolism; D000107:Acetylation; D000814:Aniline Compounds / Q000378:metabolism; D001191:Arylamine N-Acetyltransferase / Q000032:analysis; D006019:Glycolysis / Q000502:physiology*; D006801:Humans; D015464:Leukemia, Myelogenous, Chronic, BCR-ABL Positive / Q000201:enzymology / Q000378:metabolism; D007962:Leukocytes / Q000201:enzymology / Q000378:metabolism*; D008928:Mitochondria / Q000201:enzymology*; D010101:Oxygen Consumption / Q000502:physiology; D011768:Pyruvate Dehydrogenase Complex / Q000502:physiology*; D019289:Pyruvic Acid / Q000378:metabolism
D000814:Aniline Compounds; D011768:Pyruvate Dehydrogenase Complex; C019498:4-nitroaniline; D000105:Acetyl Coenzyme A; D019289:Pyruvic Acid; D001191:Arylamine N-Acetyltransferase