Trichoderma reesei prs12 encodes a stress- and unfolded-protein-response-inducible regulatory subunit of the fungal 26S proteasome.
We have cloned a gene, prs12, from the filamentous fungus Trichoderma reesei which encodes a fungal homologue of the mouse and Drosophila regulatory subunit 12 of the 26S proteasome (mov34). Sequencing of both a genomic and a cDNA-clone predicts a 342-aa protein with high overall identity (56-68 %) to the homologous counterparts from human, mammals, Drosophila and Saccharomyces cerevisiae. The predicted protein contains several consensus sequences for phosphorylation, three of which are conserved in all published Prs12p homologues. Its C-terminus is rich in alternating K and E/D, and resembles a potential KEKE-motif. Prs12 exhibits a basal level of transcription during normal growth, but its expression is significantly increased over 60-120 min under conditions of stress evoked by the addition of cadmium ions and hygromycin B. It is also stimulated by the addition of tunicamycin and 2-mercaptoethanol, suggesting its regulation by the presence of unfolded proteins in the endoplasmic reticulum and by hygromycin B. Consistent with this behaviour, motifs in the prs12 5'-upstream sequences show sequence homology with the consensus sequences for general stress response, and for an ER traffic-response element.
Goller
S P
SP
Section of Microbial Biochemistry, Institute of Biochemical Technology and Microbiology, Vienna, Austria.
Gorfer
M
M
Kubicek
C P
CP
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D017931:DNA Primers / Q000235:genetics; D004271:DNA, Fungal / Q000235:genetics; D015966:Gene Expression Regulation, Fungal; D005800:Genes, Fungal; D006801:Humans; D051379:Mice; D008969:Molecular Sequence Data; D010447:Peptide Hydrolases / Q000737:chemistry / Q000235:genetics*; D016133:Polymerase Chain Reaction; D011401:Promoter Regions, Genetic; D046988:Proteasome Endopeptidase Complex; D017510:Protein Folding; D017346:Protein Serine-Threonine Kinases / Q000235:genetics* / Q000378:metabolism; D029702:Schizosaccharomyces pombe Proteins; D017386:Sequence Homology, Amino Acid; D013312:Stress, Physiological; D014242:Trichoderma / Q000201:enzymology* / Q000235:genetics*
D017931:DNA Primers; D004271:DNA, Fungal; D029702:Schizosaccharomyces pombe Proteins; C113036:Sck2 protein, S pombe; D017346:Protein Serine-Threonine Kinases; D010447:Peptide Hydrolases; D046988:Proteasome Endopeptidase Complex; C061553:ATP dependent 26S protease