title: Antioxidative and superoxide scavenging activities of retrochalcones in Glycyrrhiza inflata.
abstract: Licochalcone A, B, C, D and echinatin, retrochalcones isolated from the roots of Glycyrrhiza inflata (licorice), along with an ordinary chalcone isoliquiritigenin, were assessed for their inhibitory activities on lipid peroxidation in various systems and radical scavenging activity. Among those tested, licochalcones B and D strongly inhibited superoxide anion production in the xanthine/xanthine oxidase system. These two compounds also showed potent scavenging activity on DPPH radical. Microsomal lipid peroxidation induced by Fe(III)-ADP/NAPDH was inhibited almost completely by 3 micrograms/ml of licochalcones B and D. Mitochondrial lipid peroxidation induced by Fe(III)-ADP/NADH was more sensitive to these retrochalcones; almost complete inhibition was observed at 10 micrograms/ml of all retrochalcones tested. Licochalcones B and D scavenged superoxide anion in microsome. Furthermore, these retrochalcones protected red cells against oxidative hemolysis. These phenolic compounds were shown to be effective to protect biological systems against various oxidative stresses.
authors.affiliation: Faculty of Engineering, Fukuyama University, Japan.
authors.forename: H
authors.initials: H
authors.lastname: Haraguchi
authors.forename: H
authors.initials: H
authors.lastname: Ishikawa
authors.forename: K
authors.initials: K
authors.lastname: Mizutani
authors.forename: Y
authors.initials: Y
authors.lastname: Tamura
authors.forename: T
authors.initials: T
authors.lastname: Kinoshita
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000494:pharmacology*; D002599:Chalcone / Q000031:analogs & derivatives* / Q000494:pharmacology*; D003124:Colorimetry; D016166:Free Radical Scavengers / Q000494:pharmacology*; D006035:Glycyrrhiza / Q000737:chemistry*; D006801:Humans; D015227:Lipid Peroxidation / Q000187:drug effects; D008297:Male; D051379:Mice; D008862:Microsomes, Liver / Q000187:drug effects / Q000201:enzymology / Q000378:metabolism; D010936:Plant Extracts / Q000494:pharmacology*; D010946:Plants, Medicinal; D051381:Rats; D017208:Rats, Wistar; D013329:Structure-Activity Relationship; D013481:Superoxides / Q000378:metabolism*; D014969:Xanthine Oxidase / Q000378:metabolism
chemical_list: D000975:Antioxidants; D016166:Free Radical Scavengers; D010936:Plant Extracts; D013481:Superoxides; D002599:Chalcone; D014969:Xanthine Oxidase