title: Human lymphocytes obtained from decidual tissue express killer activatory receptors as well as killer inhibitory receptors: analysis using a single strand conformation polymorphism method.
abstract: OBJECTIVE
  The objective was to clarify whether lymphocytes in decidual tissue express only killer inhibitory receptors (KIRs) or both KIRs and killer activatory receptors (KARs).
  
  
  METHODS
  A polymerase chain reaction (PCR)-single strand conformation polymorphism (SSCP) analysis method, using cDNA as a template, was applied to detect as many natural killer receptors (NKRs) expressed on natural killer (NK) cells as possible and to distinguish between KIRs and KARs. Using this method, we analyzed NKRs on lymphocytes in decidual tissue and maternal peripheral blood mononuclear cells (PBMCs) (n = 10) from the same individuals.
  
  
  RESULTS
  More than 20 SSCP bands were detected for NKRs on both the lymphocytes in decidual tissue and the maternal PBMCs. The SSCP band patterns were different for each individual. KARs mRNA was detected in lymphocytes in decidual tissue according to the SSCP analysis results.
  
  
  CONCLUSIONS
  Lymphocytes in decidual tissue express not only KIRs but also KARs; this finding suggests their roles in placentation and the maintenance of pregnancy.
authors.affiliation: Department of Obstetrics and Gynecology, Faculty of Medicine, University of Tokyo, Japan.
authors.forename: A
authors.initials: A
authors.lastname: Miki
authors.forename: Y
authors.initials: Y
authors.lastname: Ishikawa
authors.forename: T
authors.initials: T
authors.lastname: Yabe
authors.forename: H
authors.initials: H
authors.lastname: Furukawa
authors.forename: T
authors.initials: T
authors.lastname: Fujii
authors.forename: K
authors.initials: K
authors.lastname: Tokunaga
authors.forename: T
authors.initials: T
authors.lastname: Akaza
authors.forename: K
authors.initials: K
authors.lastname: Tadokoro
authors.forename: Y
authors.initials: Y
authors.lastname: Taketani
authors.forename: T
authors.initials: T
authors.lastname: Juji
mesh_terms: D000028:Abortion, Induced; D000328:Adult; D018076:DNA, Complementary; D003656:Decidua / Q000166:cytology / Q000276:immunology*; D005260:Female; D006801:Humans; D007694:Killer Cells, Natural; D008214:Lymphocytes / Q000276:immunology*; D018807:Polymorphism, Single-Stranded Conformational; D011247:Pregnancy; D011261:Pregnancy Trimester, First; D011971:Receptors, Immunologic / Q000032:analysis* / Q000235:genetics; D054340:Receptors, KIR; D055607:Receptors, Natural Killer Cell; D017422:Sequence Analysis, DNA
chemical_list: D018076:DNA, Complementary; D011971:Receptors, Immunologic; D054340:Receptors, KIR; D055607:Receptors, Natural Killer Cell