title: A HPLC method for the determination of 9-cis retinoic acid (ALRT1057) and its 4-oxo metabolite in human plasma.
abstract: A HPLC method was developed and validated for the quantitation of 9-cis-retinoic acid (ALRT1057) and its major metabolite, 4-oxo-9-cis-retinoic acid (LG100182) in human plasma. Samples were buffered and extracted with methyl-tert-butyl-ether. The analytes and an I.S. were separated on a C18 HPLC column using a shallow gradient of 70-89% organic solvent. The analytes were quantitated by UV detection at 348 nm. Selectivity against endogenous compounds and potential metabolites (retinol, all trans-, 13-cis-, and 4-hydroxy-9-cis-retinoic acid) was demonstrated. The run time was 29 min. The standard curve was linear from 2.5 to 450 ng ml-1. Interassay precision for both analytes in quality control samples was less than 5.0% RSD. Accuracy was within 11.0% RE for both compounds. Analyte stability during sample storage, extraction processing, and chromatography was established. Method ruggedness was tested by two analysts and on two HPLC systems. This method has been applied to the quantitation of clinical samples.
authors.affiliation: Harris Laboratories, Lincoln, NE 68502, USA.
authors.forename: A M
authors.initials: AM
authors.lastname: Dzerk
authors.forename: A
authors.initials: A
authors.lastname: Carlson
authors.forename: G R
authors.initials: GR
authors.lastname: Loewen
authors.forename: M A
authors.initials: MA
authors.lastname: Shirley
authors.forename: J W
authors.initials: JW
authors.lastname: Lee
mesh_terms: D000077556:Alitretinoin; D002138:Calibration; D002851:Chromatography, High Pressure Liquid; D006801:Humans; D007202:Indicators and Reagents; D011786:Quality Control; D012015:Reference Standards; D015203:Reproducibility of Results; D012996:Solutions; D014212:Tretinoin / Q000031:analogs & derivatives* / Q000097:blood*
chemical_list: D007202:Indicators and Reagents; C112414:LG 100182; D012996:Solutions; D000077556:Alitretinoin; D014212:Tretinoin