Implications for cAMP-dependent protein kinase in the maintenance of the interphase state.
The cAMP dependent protein kinase (A-kinase) is one of the first and best studied kinases in mammalian cells. There is extensive evidence that A-kinase activity acts antagonistically toward mitotic entry both in oocyte and somatic cells. Firstly, A-kinase seems to directly compromise the activation process of the cdc2 cyclin B mitotic kinase. Secondly, as shown by specific in vivo inhibition of A-kinase using microinjection of a stable form of its inhibitor peptide PKI, A-kinase modulates several key interphase cellular processes including cytoskeletal dynamics, transcription, chromatin structure and nuclear localization. We discuss the potential mechanisms involved in the down regulation of A-kinase activity at the interphase/mitosis transition.
Fernandez
A
A
Centre de Recherches de Biochimie Macromoléculaire, CNRS-INSERM, Montpellier, France.
Cavadore
J C
JC
Demaille
J
J
Lamb
N
N
D000818:Animals; D016203:CDC2 Protein Kinase / Q000378:metabolism; D002467:Cell Nucleus / Q000201:enzymology; D002478:Cells, Cultured; D002843:Chromatin / Q000502:physiology; D000242:Cyclic AMP / Q000378:metabolism; D017868:Cyclic AMP-Dependent Protein Kinases / Q000378:metabolism*; D015536:Down-Regulation; D005347:Fibroblasts; D005786:Gene Expression Regulation; D007382:Intermediate Filaments / Q000502:physiology; D007399:Interphase; D008322:Mammals; D008938:Mitosis; D014158:Transcription, Genetic
D002843:Chromatin; D000242:Cyclic AMP; D017868:Cyclic AMP-Dependent Protein Kinases; D016203:CDC2 Protein Kinase