Vaccination with DNA encoding an immunodominant myelin basic protein peptide targeted to Fc of immunoglobulin G suppresses experimental autoimmune encephalomyelitis.
We explore here if vaccination with DNA encoding an autoantigenic peptide can suppress autoimmune disease. For this purpose we used experimental autoimmune encephalomyelitis (EAE), which is an autoaggressive disease in the central nervous system and an animal model for multiple sclerosis. Lewis rats were vaccinated with DNA encoding an encephalitogenic T cell epitope, guinea pig myelin basic protein peptide 68-85 (MBP68-85), before induction of EAE with MBP68-85 in complete Freund's adjuvant. Compared to vaccination with a control DNA construct, the vaccination suppressed clinical and histopathological signs of EAE, and reduced the interferon gamma production after challenge with MBP68-85. Targeting of the gene product to Fc of IgG was essential for this effect. There were no signs of a Th2 cytokine bias. Our data suggest that DNA vaccines encoding autoantigenic peptides may be useful tools in controlling autoimmune disease.
Lobell
A
A
Microbiology and Tumorbiology Center, Karolinska Institute, S-171 77 Stockholm, Sweden. anna.lobell@eu.pnu.com
Weissert
R
R
Storch
M K
MK
Svanholm
C
C
de Graaf
K L
KL
Lassmann
H
H
Andersson
R
R
Olsson
T
T
Wigzell
H
H
D000818:Animals; D001327:Autoimmune Diseases / Q000276:immunology; D004247:DNA / Q000276:immunology; D004679:Encephalomyelitis / Q000276:immunology* / Q000503:physiopathology; D006168:Guinea Pigs; D007141:Immunoglobulin Fc Fragments / Q000276:immunology*; D004676:Myelin Basic Protein / Q000276:immunology*; D010446:Peptide Fragments / Q000276:immunology; D010957:Plasmids / Q000235:genetics; D051381:Rats; D011917:Rats, Inbred Lew; D013601:T-Lymphocytes / Q000276:immunology; D019444:Vaccines, DNA / Q000276:immunology* / Q000627:therapeutic use
D007141:Immunoglobulin Fc Fragments; D004676:Myelin Basic Protein; D010446:Peptide Fragments; D019444:Vaccines, DNA; D004247:DNA