Nociceptin hyperpolarises neurones in the rat ventromedial hypothalamus.
Patch-clamp recording techniques were used to examine the effect of nociceptin upon neurones contained in slices from the rat ventromedial hypothalamus (VMH). Bath application of 50-300 nM nociceptin hyperpolarised neurones in a concentration-dependent manner that was not affected by either tetrodotoxin (TTX) or naloxone. In voltage-clamp studies nociceptin induced an outward current at -60mV that had a reversal potential of -100.2 +/- 4.6 mV and was abolished by bath application of 2 mM BaCl2.
Lee
K
K
Parke Davis Neuroscience Research Centre, Cambridge University Forvie Site, UK.
Nicholson
J R
JR
McKnight
A T
AT
D000818:Animals; D007033:Hypothalamus, Middle / Q000166:cytology / Q000187:drug effects*; D066298:In Vitro Techniques; D008297:Male; D008564:Membrane Potentials / Q000187:drug effects; D009270:Naloxone / Q000494:pharmacology; D009474:Neurons / Q000187:drug effects*; D018847:Opioid Peptides / Q000494:pharmacology*; D018408:Patch-Clamp Techniques; D051381:Rats; D017207:Rats, Sprague-Dawley; D013779:Tetrodotoxin / Q000494:pharmacology; D000097629:Nociceptin
D009270:Naloxone; D018847:Opioid Peptides; D013779:Tetrodotoxin; D000097629:Nociceptin