Effects of cis-diamminedichloroplatinum(II) on Escherichia coli and bacteriophage systems.
The effects of cis-diamminedichloroplatinum(II) (cisplatin) on Escherichia coli cells and bacteriophages were investigated. The bacteriocidal effect of cisplatin was stronger on uvrA or recA mutants than on wild type cells. The drug, like UV, induced prophage development in lysogenic bacteria. Host cell reactivation of alpha3 replicative form (RF) I DNA treated with cisplatin in vitro was more efficient in wild type or recA cells than in uvrA host. When wild type cells were exposed to cisplatin, decay of the host's capacity to sustain the viral multiplication proceeded nearly in parallel with the loss of colony-forming ability, whereas the capacity of uvrA mutant was much more resistant to the drug, as compared with the viability. In the DNA preparation from cisplatin-treated alpha3-infected wild type cells, RF II was deficient, but the RF I molecules extracted from the cells were moderately infective. The microvirid gene A protein, required for RF I-->RF II conversion, was hardly detectable in wild type cells exposed to cisplatin. The possible relationship between uvr+-dependent repair and synthesis of the viral protein is discussed.
Tanaka
N
N
Departments of Otorhinolaryngology and Biochemistry I, Fukui Medical University, Matsuoka-cho, Yoshida-gun, Fukui 910-1193, Japan. nobuyuki@fmsrsa.fukui-med.ac.jp
Saito
H
H
Kimoto
H
H
Taketo
A
A
D000970:Antineoplastic Agents / Q000494:pharmacology*; D010582:Bacteriophage lambda / Q000187:drug effects* / Q000254:growth & development / Q000378:metabolism; D010584:Bacteriophage phi X 174 / Q000187:drug effects* / Q000254:growth & development / Q000378:metabolism; D002945:Cisplatin / Q000494:pharmacology*; D004261:DNA Replication / Q000187:drug effects; D004269:DNA, Bacterial / Q000037:antagonists & inhibitors; D004279:DNA, Viral / Q000096:biosynthesis / Q000737:chemistry; D004926:Escherichia coli / Q000187:drug effects* / Q000254:growth & development / Q000378:metabolism; D005814:Genes, Viral; D009154:Mutation; D014162:Transfection / Q000187:drug effects; D014764:Viral Proteins / Q000037:antagonists & inhibitors; D014775:Virus Activation / Q000187:drug effects; D014779:Virus Replication / Q000187:drug effects
D000970:Antineoplastic Agents; D004269:DNA, Bacterial; D004279:DNA, Viral; D014764:Viral Proteins; D002945:Cisplatin