Allele-specific HLA-B*15 typing by PCR-SSP and its application to four distinct ethnic populations.
We present a set of primer mixes for the allele-specific typing of the HLA-B*15 group by PCR-SSP. The set comprises 46 primer mixes which are designed to unequivocally resolve all but two of the 666 possible combinations of the B*15 alleles, B*1501-37 (B*1536 sequence unavailable). A core subset of 34 of the 46 mixes can be used alone to give a high resolution B*15 typing set. This allows for the identification of each B*15 allele when present as the only B*15 allele and the majority of the possible B*15 homozygotic combinations. The method was validated using reference DNA samples and the B*15 allele frequency in 4 distinct ethnic populations was investigated. The results show that these populations contain predominantly mutually exclusive sets of B*15 alleles.
Barnardo
M C
MC
Transplantation Immunology, Oxford Transplant Centre, Churchill Hospital, United Kingdom. mbarnard@hgmp.mrc.ac.uk
Welsh
K I
KI
Vilches
C
C
Maitland
K
K
Bunce
M
M
D000483:Alleles; D017931:DNA Primers; D005006:Ethnicity / Q000235:genetics*; D015235:HLA-B Antigens / Q000235:genetics*; D059910:HLA-B15 Antigen; D006650:Histocompatibility Testing; D006801:Humans; D016133:Polymerase Chain Reaction / Q000379:methods*
D017931:DNA Primers; D015235:HLA-B Antigens; D059910:HLA-B15 Antigen