Urinary NO2- and NO3- evaluation by an ion chromatography system.
We describe an ion chromatography system using a Dionex AS4A-SC column with carbonate-bicarbonate buffer (1.8-1.7 mM) as eluent for the evaluation of urinary NO2- and NO3-. This chromatographic system gives an accurate measurement of NO2- and NO3- in the urine as an index of NO production in vivo, making also possible to evaluate their relative proportion and providing useful tools to investigate the NO system.
Calo
L
L
Institute of Internal Medicine, University of Padova, Italy.
Cantaro
S
S
Paleari
D
D
Vianello
D
D
Zerbo
F
F
Bonfante
L
L
Favaro
S
S
Antonello
A
A
D'Angelo
A
A
D000328:Adult; D000838:Anions; D002853:Chromatography, Liquid / Q000379:methods*; D004563:Electrochemistry; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D009566:Nitrates / Q000652:urine*; D009573:Nitrites / Q000032:analysis*; D012016:Reference Values
D000838:Anions; D009566:Nitrates; D009573:Nitrites