title: Chylomicron remnant uptake is regulated by the expression and function of heparan sulfate proteoglycan in hepatocytes.
abstract: Chylomicron remnants transport cholesterol from the intestine, and are removed from the circulation principally by the liver. While hepatic receptors, including the low density lipoprotein (LDL) receptor account for endocytosis, heparan sulfate proteoglycans (HSPG) participate in the initial binding of remnants to liver cells. To explore the interactions between HSPG and endocytosis of remnants, in the present study the expression of HSPG was inhibited in HepG2 cells transfected by a synthetic antisense oligodeoxynucleotide SYN5. Immunofluorescent staining by a monoclonal anti-syndecan antibody showed significant reduction in the expression of syndecan in SYN5-treated cells compared with control cells. Remnant binding decreased by about 50-70% in SYN5-transfected cells. Monoclonal antibodies to either heparan sulphate or the LDL receptor decreased binding by about 60-65%. The glycosylation inhibitor beta-nitrophenylxylopyranoside inhibited remnant uptake by 25%, whereas 4-nitrophenyl-beta-D-galactopyranoside had no effect on remnant binding. Heparinase completely abolished binding at appropriate concentrations. Heparitinase was less effective than hep arinase in inhibiting remnant binding. Suramin completely abolished the remnant binding. Poly-arginine, poly-lysine, and protamine all reduced remnant uptake by the cells, as did polybrene, a synthetic polycation, suggesting a role of cation-anion interactions in remnant binding. Brefeldin A, colchicine, and monensin caused the fluorescence associated with remnants to persist within the cells, confirming that blockers of tubulovesicular processes and Golgi function inhibit the intracellular transport and degradation of the remnants. Our results show that remnant binding to liver cells depends on the LDL receptor, on the expression of HSPG core proteins, and on the functionality of heparan sulfate in HSPG.
authors.affiliation: Department of Physiology, The University of Western Australia, Nedlands.
authors.forename: B J
authors.initials: BJ
authors.lastname: Zeng
authors.forename: B C
authors.initials: BC
authors.lastname: Mortimer
authors.forename: I J
authors.initials: IJ
authors.lastname: Martins
authors.forename: U
authors.initials: U
authors.lastname: Seydel
authors.forename: T G
authors.initials: TG
authors.lastname: Redgrave
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal; D001053:Apolipoproteins / Q000032:analysis; D001692:Biological Transport / Q000187:drug effects; D002914:Chylomicrons / Q000378:metabolism*; D004705:Endocytosis; D005456:Fluorescent Dyes; D006027:Glycosides / Q000494:pharmacology; D006031:Glycosylation / Q000187:drug effects; D019812:Heparan Sulfate Proteoglycans / Q000235:genetics / Q000378:metabolism*; D019763:Heparin Lyase / Q000494:pharmacology; D008055:Lipids / Q000032:analysis; D008099:Liver / Q000166:cytology / Q000378:metabolism*; D008297:Male; D008562:Membrane Glycoproteins / Q000235:genetics / Q000378:metabolism*; D016376:Oligonucleotides, Antisense; D011133:Polysaccharide-Lyases / Q000494:pharmacology; D011509:Proteoglycans / Q000235:genetics / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar; D011973:Receptors, LDL / Q000276:immunology / Q000378:metabolism; D013498:Suramin / Q000494:pharmacology; D053667:Syndecans
chemical_list: D000911:Antibodies, Monoclonal; D001053:Apolipoproteins; D002914:Chylomicrons; D005456:Fluorescent Dyes; D006027:Glycosides; D019812:Heparan Sulfate Proteoglycans; D008055:Lipids; D008562:Membrane Glycoproteins; D016376:Oligonucleotides, Antisense; D011509:Proteoglycans; D011973:Receptors, LDL; D053667:Syndecans; D013498:Suramin; D011133:Polysaccharide-Lyases; D019763:Heparin Lyase; C021349:heparitinsulfate lyase