Genetic analysis of hydroxymethylglutaryl-coenzyme A reductase regulated degradation.
Hydroxymethylglutaryl-coenzyme A reductase degradation occurs in the endoplasmic reticulum, and is regulated by the mevalonate pathway. In order to discover the molecules that mediate the degradation process and its control, we conducted a genetic analysis of the degradation of the yeast Hmg2p isozyme of hydroxymethylglutaryl-coenzyme A reductase. Hmg2p degradation occurs by the action of HRD genes that direct Hmg2p to the ubiquitin-proteasome pathway. Regulation of HRD-dependent Hmg2p degradation appears to occur by the action of a separate set of CRD genes.
Hampton
R Y
RY
UCSD Department of Biology 0347, La Jolla 92093, USA. rhampton@biomall.ucsd.edu
D000818:Animals; D003546:Cysteine Endopeptidases / Q000378:metabolism; D004721:Endoplasmic Reticulum / Q000201:enzymology; D005204:Farnesol / Q000378:metabolism; D015971:Gene Expression Regulation, Enzymologic; D006868:Hydrolysis; D006903:Hydroxymethylglutaryl CoA Reductases / Q000378:metabolism*; D009097:Multienzyme Complexes / Q000378:metabolism; D046988:Proteasome Endopeptidase Complex; D015398:Signal Transduction; D014452:Ubiquitins / Q000378:metabolism
D009097:Multienzyme Complexes; D014452:Ubiquitins; D005204:Farnesol; D006903:Hydroxymethylglutaryl CoA Reductases; D003546:Cysteine Endopeptidases; D046988:Proteasome Endopeptidase Complex