title: Regeneration of the hepatic nerves following surgical denervation of the liver in dogs.
abstract: This study was conducted to examine the regeneration process of hepatic nerves following surgical hepatic denervation in dogs. A denervation model was surgically created by removing all visible nerves around the hepatoduodenal ligament along with the peri-hepatic tissues. The hepatic nerves were examined on the hepatic specimens taken at 1 week, 1 month and 3 months post-denervation by means of immunohistochemical staining, and also electron microscopy. At 1 week post-denervation, the extrinsic hepatic nerves were observed not to have regenerated. However, at 1 month post-denervation, GAP-43-positive nerves were identified and regenerating axons were shown to be present on electron microscopic observation. The immunoreactivity for anti-GAP-43 antibody was not shown any longer at 3 months post-denervation, and the regenerated nerve axons were shown to be similar to those at pre-denervation on ultrastructural study. Results of the present study suggested that regeneration of the extrinsic hepatic nerves began to appear about 1 month after the hepatic denervation, and was completed by 3 months post-denervation.
authors.affiliation: Department of Surgery, Kitasato University School of Medicine, Sagamihara, Kanagawa, Japan.
authors.forename: Y
authors.initials: Y
authors.lastname: Ito
authors.forename: T
authors.initials: T
authors.lastname: Takahashi
authors.forename: F
authors.initials: F
authors.lastname: Tadokoro
authors.forename: K
authors.initials: K
authors.lastname: Hayashi
authors.forename: Z
authors.initials: Z
authors.lastname: Iino
authors.forename: K
authors.initials: K
authors.lastname: Sato
authors.forename: K
authors.initials: K
authors.lastname: Akira
mesh_terms: D000818:Animals; D017763:Autonomic Denervation; D001341:Autonomic Nervous System / Q000502:physiology* / Q000648:ultrastructure; D004285:Dogs; D005260:Female; D019922:GAP-43 Protein / Q000378:metabolism; D007124:Immunoenzyme Techniques; D008099:Liver / Q000294:innervation* / Q000601:surgery; D008297:Male; D009412:Nerve Fibers / Q000378:metabolism / Q000648:ultrastructure; D009416:Nerve Regeneration / Q000502:physiology*; D013869:Thiolester Hydrolases / Q000378:metabolism; D043222:Ubiquitin Thiolesterase
chemical_list: D019922:GAP-43 Protein; D013869:Thiolester Hydrolases; D043222:Ubiquitin Thiolesterase