Phosphorylation of elongation factor-1 (EF-1) by cdc2 kinase.
Elongation factor-1 (EF-1) is a major substrate for cdc2 kinase in Xenopus oocytes. The guanine-nucleotide exchange factor EF-1 beta gamma delta, appears to have a highly complex macromolecular structure containing several GTP/GDP exchange proteins, valyl-tRNA synthetase, and a putative anchoring protein EF-1 gamma. During meiotic cell division, the factor becomes phosphorylated by cdc2 kinase, not only on EF-1 gamma, but also on two different phospho-acceptors on EF-1 delta. Phosphorylation is concomitant with changes in protein synthesis in vivo. Xenopus oocytes, and potentially all cells, contain a multitude of heteromeric forms of the complex which postulates that EF-1 beta gamma delta is not a "house keeping" factor but a sophisticated regulatory element.
Bellé
R
R
Biologie Cellulaire de l'Ovocyte, CNRS URA 1449, INRA, Université Pierre et Marie Curie, Paris, France.
Minella
O
O
Cormier
P
P
Morales
J
J
Poulhe
R
R
Mulner-Lorillon
O
O
D000818:Animals; D016203:CDC2 Protein Kinase / Q000502:physiology*; D008540:Meiosis; D009865:Oocytes; D020648:Peptide Elongation Factor 1; D010445:Peptide Elongation Factors / Q000378:metabolism*; D010766:Phosphorylation; D014981:Xenopus
D020648:Peptide Elongation Factor 1; D010445:Peptide Elongation Factors; D016203:CDC2 Protein Kinase