Construction of a recombinant feline herpesvirus type 1 expressing Gag precursor protein of feline immunodeficiency virus.
We constructed a deletion mutant of feline herpesvirus type 1 (FHV-1) and a recombinant FHV-1. The deletion mutant is the virus with a region (367 bp) deleted from the start codon of thymidine kinase (TK) gene to the SmaI site within the TK gene, and the other is a recombinant FHV-1 expressing Gag protein of feline immunodeficiency virus (FIV), in which a cDNA encoding the Gag protein of FIV was inserted at the TK deletion site of the former deletion mutant. These viruses were designated as C7301ddlTK and C7301ddlTK-gag, respectively. Growth kinetics of these viruses in Crandell feline kidney cells was similar to that of the parent C7301 strain. By immunoblot analysis, C7301 ddlTK-gag was confirmed to express the FIV Gag precursor protein in the cells.
Sato
E
E
Department of Veterinary Microbiology, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Japan.
Yokoyama
N
N
Maeda
K
K
Inoshima
Y
Y
Kohmoto
M
M
Ikeda
Y
Y
Miyazawa
T
T
Mikami
T
T
D018138:Alphaherpesvirinae / Q000235:genetics; D000818:Animals; D002415:Cats; D002460:Cell Line; D003001:Cloning, Molecular; D017353:Gene Deletion; D015870:Gene Expression; D015683:Gene Products, gag / Q000235:genetics*; D005822:Genetic Vectors; D006801:Humans; D016089:Immunodeficiency Virus, Feline / Q000235:genetics*; D011498:Protein Precursors / Q000235:genetics*; D011993:Recombinant Fusion Proteins / Q000235:genetics; D011995:Recombination, Genetic; D013937:Thymidine Kinase / Q000235:genetics; D014407:Tumor Cells, Cultured; D014764:Viral Proteins
C069338:FIV gag precursor protein, Feline immunodeficiency virus; D015683:Gene Products, gag; D011498:Protein Precursors; D011993:Recombinant Fusion Proteins; D014764:Viral Proteins; D013937:Thymidine Kinase