Molecular heterogeneity of Vicia villosa-recognized perineuronal nets surrounding pyramidal and nonpyramidal neurons in the guinea pig cerebral cortex.
Of three monoclonal antibodies (mAbs Cat 301, 1B5 and 473) which recognize epitopes on perineuronal nets (PnNs), only mAb 473 displayed considerably varied degrees of staining intensity on Vicia villosa-labeled pyramidal (P) neurons (5%, intensely; 54%, very weakly; and 41%, unstained). These results indicate the heterogeneity on molecular structure or composition of the terminal N-acetylgalactosamine-containing PnNs within P class as well as between P and nonpyramidal classes.
Ojima
H
H
1st Department of Anatomy, Toho University School of Medicine, 5-21-16, Omori-Nishi, Ota-ku, Tokyo 143, Japan. yojima@med.toho-u.ac.jp
Sakai
M
M
Ohyama
J
J
D000818:Animals; D000911:Antibodies, Monoclonal; D002540:Cerebral Cortex / Q000166:cytology / Q000378:metabolism*; D005260:Female; D006168:Guinea Pigs; D006651:Histocytochemistry; D007150:Immunohistochemistry; D037102:Lectins; D008297:Male; D008856:Microscopy, Fluorescence; D017350:N-Acetylgalactosaminyltransferases / Q000378:metabolism; D009415:Nerve Net / Q000502:physiology*; D009474:Neurons / Q000378:metabolism; D037121:Plant Lectins; D017966:Pyramidal Cells / Q000378:metabolism*; D000097763:Polypeptide N-acetylgalactosaminyltransferase
D000911:Antibodies, Monoclonal; D037102:Lectins; D017350:N-Acetylgalactosaminyltransferases; D037121:Plant Lectins; D000097763:Polypeptide N-acetylgalactosaminyltransferase; C042242:Vicia lectins; C023220:(N-acetylneuraminyl)-galactosylglucosylceramide N-acetylgalactosaminyltransferase