Lead induced disorders in hematopoietic and drug metabolizing enzyme system and their protection by ascorbic acid supplementation.
Effect of vitamin C supplementation in restoring lead induced alterations in hematopoietic system and drug metabolizing enzymes were investigated in male rats. Intraperitoneal administration of 20 mg/kg lead produced a significant inhibition of heme synthesis in blood and liver and drug metabolism in liver. Toxic insult by lead also resulted into a marked decline in tissue thiols and vitamin C levels. Oral supplementation of vitamin C (100 mg/kg for 3 days) completely restored blood delta aminolevulinic acid dehydratase, uroporphyrinogen I synthetase and a few drug metabolizing enzymes. Level of vitamin C and sulfhydryl contents too recovered to a great extent. A marked reduction in blood and liver lead concentration occurred on vitamin C supplementation although renal lead contents were marginally reduced in lead exposed animals. The results, thus, indicate a significant protective action of vitamin C against toxic effects of lead on heme synthesis and drug metabolism.
Vij
A G
AG
Defence Institute of Physiology and Allied Sciences, Timarpur, Delhi, India.
Satija
N K
NK
Flora
S J
SJ
D000284:Administration, Oral; D000815:Aniline Hydroxylase / Q000097:blood; D000818:Animals; D001205:Ascorbic Acid / Q000008:administration & dosage / Q000378:metabolism / Q000494:pharmacology*; D006410:Hematopoiesis / Q000187:drug effects; D006418:Heme / Q000096:biosynthesis*; D011163:Hydroxymethylbilane Synthase / Q000097:blood; D007668:Kidney / Q000187:drug effects / Q000201:enzymology; D007854:Lead / Q000097:blood / Q000633:toxicity*; D007855:Lead Poisoning / Q000097:blood / Q000201:enzymology*; D008099:Liver / Q000187:drug effects* / Q000201:enzymology; D008297:Male; D008862:Microsomes, Liver / Q000187:drug effects / Q000201:enzymology; D006899:Mixed Function Oxygenases / Q000378:metabolism; D000623:Porphobilinogen Synthase / Q000097:blood; D051381:Rats; D013438:Sulfhydryl Compounds / Q000378:metabolism
D013438:Sulfhydryl Compounds; D007854:Lead; D006418:Heme; D006899:Mixed Function Oxygenases; D000815:Aniline Hydroxylase; D011163:Hydroxymethylbilane Synthase; D000623:Porphobilinogen Synthase; D001205:Ascorbic Acid