Distinct combination of purification methods dramatically improves cohesive-end subcloning of PCR products.
Wybranietz
W A
WA
Dept. Internal Medicine I, Medical University Clinic Tübingen, Germany.
Lauer
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D003001:Cloning, Molecular / Q000379:methods*; D004247:DNA / Q000302:isolation & purification; D004262:DNA Restriction Enzymes / Q000378:metabolism; D004587:Electrophoresis, Agar Gel; D019286:Endopeptidase K / Q000378:metabolism; D005784:Gene Amplification / Q000235:genetics; D007763:Lac Operon / Q000235:genetics; D019913:Nuclear Localization Signals / Q000235:genetics; D016133:Polymerase Chain Reaction / Q000379:methods*; D019914:Taq Polymerase / Q000378:metabolism
D019913:Nuclear Localization Signals; D004247:DNA; D019914:Taq Polymerase; D004262:DNA Restriction Enzymes; D019286:Endopeptidase K