Leukotriene D4-induced signalling events in human epithelial cells: G alpha i3 activation and translocation.
Our model of LTD4-induced signal transduction in epithelial cells is summarised in Figure 2. Extending what is already known about LTD4 signalling in epithelial cells, we identified the Gi3-protein as the crucial PTX sensitive G-protein and found that it is translocated to what might be a cytoskeletal fraction. This finding suggests a subtle response to LTD4, mediated via the bifurcation at the alpha/beta gamma junction. Although little is known about the role of epithelial cells in inflammation, it has been shown that such cells produce the potent chemoattractant LTB4 and the proinflammatory 5-HETE in response to intracellular accumulation of Ca2+ 24. The target protein(s) and the effect(s) of the translocation of the activated G alpha i3-proteins, as well as the possible role of the beta/gamma-subunits of Gi3, remain to be elucidated.
Ohd
J F
JF
Department of Laboratory Medicine, Lund University, Wallenberg Laboratory, UMAS, Malmö, Sweden.
Adolfsson
J L
JL
Sjölander
A
A
D000818:Animals; D004847:Epithelial Cells / Q000187:drug effects / Q000502:physiology*; D019206:GTP-Binding Protein alpha Subunits, Gi-Go / Q000378:metabolism*; D006801:Humans; D017998:Leukotriene D4 / Q000494:pharmacology*; D046911:Macromolecular Substances; D008954:Models, Biological; D009504:Neutrophils / Q000502:physiology; D051381:Rats; D015398:Signal Transduction / Q000187:drug effects* / Q000502:physiology
D046911:Macromolecular Substances; D017998:Leukotriene D4; D019206:GTP-Binding Protein alpha Subunits, Gi-Go