Use of random amplified polymorphic DNA for rapid molecular subtyping of Corynebacterium diphtheriae.
A total of 210 Corynebacterium diphtheriae strains isolated worldwide were assayed by random amplified polymorphic DNA (RAPD) assay. RAPD was as discriminating as standard ribotyping, and in some cases, even further differentiation was obtained. RAPD can rapidly aid clinical and molecular epidemic studies in a simple and cost-effective manner.
Nakao
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Division of Bacterial and Mycotic Diseases, National Center for Infectious Diseases, Centers for Disease Control and Prevention, Public Health Service, U.S. Department of Health and Human Services, Atlanta, Georgia 30333, USA.
Popovic
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D015373:Bacterial Typing Techniques; D003353:Corynebacterium diphtheriae / Q000235:genetics*; D003362:Cost-Benefit Analysis; D004269:DNA, Bacterial / Q000145:classification / Q000235:genetics*; D006801:Humans; D019105:Random Amplified Polymorphic DNA Technique / Q000191:economics; D017422:Sequence Analysis, DNA
D004269:DNA, Bacterial