Random amplification of polymorphic DNA (RAPD), pulsed-field gel electrophoresis (PFGE) and phage-typing in the analysis of a hospital outbreak of Salmonella enteritidis.
Isolates of Salmonella Enteritidis from 81 patients from Herlev Hospital or from Copenhagen County were analysed by random amplification of polymorphic DNA (RAPD), pulsed-field gel electrophoresis (PFGE) and phage-typing. Fourteen polymorphic markers from five decamer primers unambiguously placed all isolates into six RAPD groups: 65 isolates of phagetype 6 (PFGE type I) were resolved into three RAPD groups constituting 86, 12, and 2%, respectively. A fourth RAPD group of 10 isolates was coincident with phage type 8 (PFGE type II) and two isolates, one phage-type 1, the other phage-type 4 (both PFGE type I) formed the fifth group. The sixth group of four isolates was not phage typeable and was PFGE type III. Forty outbreak-related isolates of phage-type 6 were resolved into three strains. No diversity of phage-type 6 was found among isolates unrelated to the outbreak. It is concluded that RAPD is useful as a tool in investigations of microbial outbreaks in its own right, or to supplement phage-typing and PFGE of Salmonella Enteritidis.
Skibsted
U
U
Department of Clinical Microbiology, Herlev Hospital, Denmark.
Baggesen
D L
DL
Dessau
R
R
Lisby
G
G
D001434:Bacteriophage Typing / Q000379:methods; D003428:Cross Infection / Q000382:microbiology*; D004269:DNA, Bacterial / Q000032:analysis*; D004196:Disease Outbreaks; D016521:Electrophoresis, Gel, Pulsed-Field / Q000379:methods; D006801:Humans; D015994:Incidence; D017053:Infection Control; D019105:Random Amplified Polymorphic DNA Technique; D015203:Reproducibility of Results; D012480:Salmonella Infections / Q000382:microbiology*; D012477:Salmonella enteritidis / Q000145:classification* / Q000235:genetics
D004269:DNA, Bacterial