Effect of B. subtilis TRNA(Trp) on readthrough rate at an opal UGA codon.
Bacillus subtilis has been thought to have a high readthrough rate at the UGA stop codon because no opal suppressor tRNA has been isolated so far [Lovett et al. (1991) J. Bacteriol. 173, 1810-1812]. To examine whether a tRNATrp which we have characterized [Matsugi et al. (1992) Nucleic Acids Res. 20, 3514] has the ability to read the UGA codon, in vitro translation was performed with a synthetic mRNA containing a test codon, UGA, UAG, UAA, or UGG, in a reading frame. Addition of Trp-tRNATrp to the system significantly increased the readthrough rate only in the case of UGA. This suggests that this tRNATrp has a dual recognition pattern in B. subtilis, i.e., for the canonical tryptophan codon and for readthrough at the UGA stop codon.
Matsugi
J
J
Laboratory of Chemistry, Jichi Medical School, 3311-1 Yakushiji, Minamikawachi-machi, Tochigi 329-0498, Japan. jmatsugi@jichi.ac.jp
Murao
K
K
Ishikura
H
H
D000926:Anticodon / Q000235:genetics; D001412:Bacillus subtilis / Q000235:genetics*; D001483:Base Sequence; D018388:Codon, Terminator / Q000235:genetics*; D009690:Nucleic Acid Conformation; D010957:Plasmids; D014176:Protein Biosynthesis; D012333:RNA, Messenger / Q000235:genetics; D012362:RNA, Transfer, Ser / Q000235:genetics; D012364:RNA, Transfer, Trp / Q000737:chemistry / Q000235:genetics*; D016364:Reading Frames; D014158:Transcription, Genetic
D000926:Anticodon; D018388:Codon, Terminator; D012333:RNA, Messenger; D012362:RNA, Transfer, Ser; D012364:RNA, Transfer, Trp