title: Analysis and overexpression in Escherichia coli of a staphylococcal gene encoding seryl-tRNA synthetase.
abstract: We have sequenced and expressed in Escherichia coli the gene encoding the seryl-tRNA synthetase from the pathogenic bacterium Staphylococcus aureus. The overexpressed and purified recombinant enzyme was able to aminoacylate unfractionated tRNA from E. coli. Its activity was not affected by antibodies raised against and inhibiting the E. coli seryl-tRNA synthetase.
authors.affiliation: European Molecular Biology Laboratory, Grenoble Outstation, B.P. 156, 38042 Grenoble Cedex, France.
authors.forename: N
authors.initials: N
authors.lastname: Bausch
authors.forename: L
authors.initials: L
authors.lastname: Seignovert
authors.forename: M
authors.initials: M
authors.lastname: Beaulande
authors.forename: R
authors.initials: R
authors.lastname: Leberman
authors.forename: M
authors.initials: M
authors.lastname: Härtlein
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D004926:Escherichia coli / Q000235:genetics; D005798:Genes, Bacterial; D007074:Immunoglobulin G / Q000494:pharmacology; D008969:Molecular Sequence Data; D011994:Recombinant Proteins; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D012714:Serine-tRNA Ligase / Q000235:genetics* / Q000276:immunology; D013211:Staphylococcus aureus / Q000201:enzymology / Q000235:genetics*
chemical_list: D007074:Immunoglobulin G; D011994:Recombinant Proteins; D012714:Serine-tRNA Ligase