Effect of mycotoxins isolated from Penicillium nigricans on glucose-6-phosphate dehydrogenase.
A new mycotoxin product (NMP) was isolated from the culture of mutated wild strain of P. nigricans which is less toxic and has sterol derivative. NMP (LD50 > 1 g/kg) showed antimicrobial and antineoplastic activities and does not affect the hematological parameters like RBC count and hemoglobin. It maintained normal blood glucose level by increasing the enzyme activity of glucose-6-phosphate dehydrogenase (EC-1.1.1.49; G-6-PDH) by 30%. It also maintained the normal ion balance in the blood of mice. NMP decreased Km value of glucose-6-phosphate dehydrogenase and thus increased substrate affinity of the enzyme. Reduction of toxicity of NMP has been well explained by higher activity of G-6-PDH which is highly specific for production of NADPH.
Majumder
U K
UK
Department of Pharmaceutical Technology, Jadavpur University, Calcutta, India.
Gupta
M
M
Mukhopadhyay
D K
DK
D000818:Animals; D000880:Anthraquinones / Q000494:pharmacology*; D005954:Glucosephosphate Dehydrogenase / Q000187:drug effects / Q000378:metabolism*; D007700:Kinetics; D051379:Mice; D009183:Mycotoxins / Q000302:isolation & purification* / Q000494:pharmacology*; D010407:Penicillium / Q000201:enzymology*
D000880:Anthraquinones; D009183:Mycotoxins; C047332:MT-81 toxin; D005954:Glucosephosphate Dehydrogenase