title: Presence of thyrotropin receptor in infant adipocytes.
abstract: In healthy infants, the levels of TSH are known to peak at 50-100 times adult values during the first days of life. In studies of isolated human infant adipocytes, we have earlier shown that bovine TSH (bTSH) has a strong lipolytic effect, accompanied by a blunted response of adipocytes to catecholamines. In this study, we used human recombinant TSH (hTSH), and incubation of adipocytes with hTSH induced a lipolytic response similar to that obtained with the beta-adrenergic receptor agonist isoprenaline in adipocytes isolated from three infants. The lipolytic effect of hTSH was completely blocked by inhibitory TSH receptor (TSHR) antibodies. The TSHR mediates the effects of TSH in the thyroid, and it has been detected in some extrathyroid tissues, but not in isolated human adipocytes or childhood adipose tissue. In this study, we found TSHR RNA in infant and adult adipose tissues and isolated adipocytes with reverse transcriptase-PCR. The sequence of the amplified PCR product agreed with the published sequence. Northern blot hybridization on RNA prepared from infant adipose tissue showed a transcript of the expected size, and the expression of TSHR seemed higher in infant than in adult adipose tissue. In conclusion, this study indicates that TSH plays an active role in the metabolic adaptation after birth.
authors.affiliation: Department of Pediatrics, Huddinge University Hospital, Karolinska Institute, Sweden.
authors.forename: A
authors.initials: A
authors.lastname: Janson
authors.forename: H
authors.initials: H
authors.lastname: Rawet
authors.forename: L
authors.initials: L
authors.lastname: Perbeck
authors.forename: C
authors.initials: C
authors.lastname: Marcus
mesh_terms: D017667:Adipocytes / Q000737:chemistry*; D000273:Adipose Tissue / Q000254:growth & development*; D000328:Adult; D000818:Animals; D015152:Blotting, Northern; D002417:Cattle; D002648:Child; D006801:Humans; D007223:Infant; D008066:Lipolysis; D016133:Polymerase Chain Reaction; D012313:RNA / Q000032:analysis; D011989:Receptors, Thyrotropin / Q000032:analysis* / Q000235:genetics; D011994:Recombinant Proteins / Q000494:pharmacology; D013972:Thyrotropin / Q000494:pharmacology
chemical_list: D011989:Receptors, Thyrotropin; D011994:Recombinant Proteins; D012313:RNA; D013972:Thyrotropin