Immunohistochemical analysis of in vivo patterns of TRAF-3 expression, a member of the TNF receptor-associated factor family.
An immunohistochemical approach was used to explore the in vivo expression of TNF receptor-associated factor 3 (TRAF-3), a putative signaling protein that binds to the cytosolic domains of CD30, CD40, and lymphotoxin-beta receptors. TRAF-3 immunostaining was detected in many types of cells throughout the human body. TRAF-3 immunostaining was only rarely present in thymocytes but was found in the thymic epithelioreticular cells. Lymphocytes in the bone marrow were also typically TRAF-3 immunonegative, whereas myeloid progenitor cells and megakaryocytes were often TRAF-3 positive. Peripheral blood lymphocytes were mostly TRAF-3 immunonegative, while granulocytes were TRAF-3 immunopositive. Monocytes were strongly immunostained for TRAF-3, but macrophages in nodes typically contained little or no TRAF-3 immunoreactivity. Some lymphocytes within the germinal centers of secondary lymphoid follicles in normal and reactive nodes were TRAF-3 immunopositive, as were occasional interfollicular lymphocytes in the T cell regions of these organs, but most lymphocytes appeared to be TRAF-3 immunonegative or stained only weakly. Plasma cells, however, were strongly TRAF-3 positive. Stimulation of PBLs with anti-CD3 Ab induced marked increases in the steady state levels of TRAF-3 protein in vitro as determined by immunoblotting, while levels of TRAF-2 were unchanged, implying a dynamic regulation of TRAF-3 expression. The findings establish for the first time the cell type- and differentiation-specific patterns of expression of a member of the TRAF family of proteins.
Krajewski
S
S
The Burnham Institute, La Jolla, CA 92037, USA.
Zapata
J M
JM
Krajewska
M
M
VanArsdale
T
T
Shabaik
A
A
Gascoyne
R D
RD
Reed
J C
JC
D000595:Amino Acid Sequence; D002319:Cardiovascular System / Q000737:chemistry; D002460:Cell Line; D004064:Digestive System / Q000737:chemistry; D004702:Endocrine Glands / Q000737:chemistry; D005260:Female; D005835:Genitalia / Q000737:chemistry; D006801:Humans; D007106:Immune Sera / Q000737:chemistry; D007150:Immunohistochemistry; D007668:Kidney / Q000737:chemistry; D007962:Leukocytes / Q000378:metabolism; D008168:Lung / Q000737:chemistry; D008214:Lymphocytes / Q000378:metabolism; D008297:Male; D008969:Molecular Sequence Data; D008970:Molecular Weight; D018482:Muscle, Skeletal / Q000737:chemistry; D009420:Nervous System / Q000737:chemistry; D009928:Organ Specificity; D014176:Protein Biosynthesis; D011506:Proteins / Q000032:analysis* / Q000276:immunology; D018124:Receptors, Tumor Necrosis Factor / Q000032:analysis* / Q000096:biosynthesis* / Q000097:blood / Q000276:immunology; D012867:Skin / Q000737:chemistry; D048008:TNF Receptor-Associated Factor 3
D007106:Immune Sera; D011506:Proteins; D018124:Receptors, Tumor Necrosis Factor; D048008:TNF Receptor-Associated Factor 3