RGS7 attenuates signal transduction through the G(alpha q) family of heterotrimeric G proteins in mammalian cells.
The RGS proteins are a recently discovered family of G protein regulators that have been shown to act as GTPase-activating proteins (GAPs) on the G(alpha i) and G(alpha q) subfamilies of the heterotrimeric G proteins. Here, we demonstrate that RGS7 is a potent GAP in vitro on G(alpha i1), and G(alpha o) heterotrimeric proteins and that RGS7 acts to down-regulate G(alpha q)-mediated calcium mobilization in a whole-cell assay system using a transient expression protocol. This RGS protein and RGS4 are reported to be expressed predominantly in brain, and in situ hybridization studies have revealed similarities in the regional distribution of RGS and G(alpha q) mRNA expression. Our findings provide further evidence to support a functional role for RGS4 and RGS7 in G(alpha q)-mediated signaling in the CNS.
Shuey
D J
DJ
Department of CNS Disorders, Wyeth-Ayerst Research, Princeton, New Jersey 08543-8000, USA.
Betty
M
M
Jones
P G
PG
Khawaja
X Z
XZ
Cockett
M I
MI
D000818:Animals; D001692:Biological Transport / Q000502:physiology; D016466:CHO Cells; D002118:Calcium / Q000378:metabolism; D006224:Cricetinae; D019204:GTP-Binding Proteins / Q000737:chemistry / Q000502:physiology*; D020690:GTPase-Activating Proteins; D006651:Histocytochemistry; D006801:Humans; D017403:In Situ Hybridization; D011506:Proteins / Q000502:physiology*; D020710:RGS Proteins; D015398:Signal Transduction / Q000187:drug effects*
D020690:GTPase-Activating Proteins; D011506:Proteins; D020710:RGS Proteins; C098799:RGS4 protein; D019204:GTP-Binding Proteins; D002118:Calcium