Highly resolved chemical imaging of living cells by using synchrotron infrared microspectrometry.
Using synchrotron radiation as an ultra-bright infrared source, we have been able to map the distributions of functional groups such as proteins, lipids, and nucleic acids inside a single living cell with a spatial resolution of a few microns. In particular, we have mapped the changes in the lipid and protein distributions in both the final stages of cell division and also during necrosis.
Jamin
N
N
Commissariat à l'Energie Atomique-Institut National des Sciences et Techniques Nucléaires, F91191 Gif Sur Yvette Cedex, France.
Dumas
P
P
Moncuit
J
J
Fridman
W H
WH
Teillaud
J L
JL
Carr
G L
GL
Williams
G P
GP
D000818:Animals; D002451:Cell Compartmentation; D016923:Cell Death; D002455:Cell Division; D006825:Hybridomas; D050356:Lipid Metabolism; D051379:Mice; D009336:Necrosis; D013055:Spectrophotometry, Infrared / Q000379:methods*; D017356:Synchrotrons