title: Calcium modulated signaling site in type 2 rod outer segment membrane guanylate cyclase (ROS-GC2).
abstract: The ROS-GC subfamily of membrane guanylate cyclases is at present represented by two members: ROS-GC1 and ROS-GC2. A unique functional feature of this subfamily is that it is intracellularly modulated in low Ca2+ concentration by calmodulin-like Ca(2+)-binding proteins termed GCAPs, 1 and 2, and the modulation is consistent with its linkage to phototransduction. The present study shows that: (1) GCAP2 is a specific modulator of ROS-GC2; (2) through systematic remodeling of ROS-GC modules, the study also shows that the modulated domain resides within the amino acid segment 736-1020. This domain is distinct form the corresponding GCAP1-modulated ROS-GC1 domain. Thus, GCAP1 and GCAP2 act through different ROS-GCs and through two different cyclase domains.
authors.affiliation: Department of Cell Biology, University of Medicine and Dentistry of New Jersey, Stratford 08084, USA.
authors.forename: R M
authors.initials: RM
authors.lastname: Goraczniak
authors.forename: T
authors.initials: T
authors.lastname: Duda
authors.forename: R K
authors.initials: RK
authors.lastname: Sharma
mesh_terms: D000818:Animals; D019556:COS Cells; D002118:Calcium / Q000494:pharmacology*; D002135:Calcium-Binding Proteins / Q000235:genetics / Q000378:metabolism / Q000494:pharmacology*; D002417:Cattle; D005136:Eye Proteins / Q000378:metabolism*; D019204:GTP-Binding Proteins / Q000235:genetics / Q000378:metabolism*; D006162:Guanylate Cyclase / Q000235:genetics / Q000378:metabolism*; D051604:Guanylate Cyclase-Activating Proteins; D020962:Heterotrimeric GTP-Binding Proteins; D009154:Mutation / Q000235:genetics; D011994:Recombinant Proteins / Q000494:pharmacology; D015293:Transducin; D014162:Transfection / Q000235:genetics; D014785:Vision, Ocular / Q000502:physiology
chemical_list: D002135:Calcium-Binding Proteins; D005136:Eye Proteins; C034809:GNAT1 protein, human; D051604:Guanylate Cyclase-Activating Proteins; D011994:Recombinant Proteins; D019204:GTP-Binding Proteins; D020962:Heterotrimeric GTP-Binding Proteins; D015293:Transducin; D006162:Guanylate Cyclase; D002118:Calcium