title: Induction of primary carcinoembryonic antigen (CEA)-specific cytotoxic T lymphocytes in vitro using human dendritic cells transfected with RNA.
abstract: Dendritic cells (DC) generated from the peripheral blood mononuclear cells of healthy individuals or from cancer patients transfected with carcinoembryonic antigen (CEA) mRNA stimulate a potent CD8+ cytotoxic T lymphocyte (CTL) response in vitro. DCs are effectively sensitized with RNA in the absence of reagents commonly used to facilitate mammalian cell transfection. RNA encoding a chimeric CEA/LAMP-1 lysosomal targeting signal enhances the induction of CEA-specific CD4+ T cells, providing a strategy to induce T-help that may be necessary to generate and/or maintain an optimal CD8+ CTL response in vivo. CEA RNA-transfected DCs also serve as effective targets in cytotoxicity assays, thus providing a general method for inducing, as well as measuring, CEA-specific CTL responses across a broad spectrum of HLA haplotypes.
authors.affiliation: Center for Genetic and Cellular Therapies, Department of Surgery, Duke University Medical Center, Durham, NC 27710, USA.
authors.forename: S K
authors.initials: SK
authors.lastname: Nair
authors.forename: D
authors.initials: D
authors.lastname: Boczkowski
authors.forename: M
authors.initials: M
authors.lastname: Morse
authors.forename: R I
authors.initials: RI
authors.lastname: Cumming
authors.forename: H K
authors.initials: HK
authors.lastname: Lyerly
authors.forename: E
authors.initials: E
authors.lastname: Gilboa
mesh_terms: D000230:Adenocarcinoma / Q000276:immunology / Q000473:pathology; D002272:Carcinoembryonic Antigen / Q000235:genetics / Q000276:immunology*; D002460:Cell Line; D003110:Colonic Neoplasms / Q000276:immunology / Q000473:pathology; D003713:Dendritic Cells / Q000276:immunology / Q000378:metabolism*; D006801:Humans; D009362:Neoplasm Metastasis / Q000276:immunology; D012313:RNA / Q000235:genetics*; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology*; D014162:Transfection; D014407:Tumor Cells, Cultured
chemical_list: D002272:Carcinoembryonic Antigen; D012313:RNA