Expression of SRY gene transcripts with a longer 3' untranslated sequence in adult human male lymphocytes and tumor cells.
Using 3' RACE PCR and the repeated nested-PCR method, the expression of transcripts of the sex-determining gene SRY was investigated in single lymphocytes from a human adult male and in male tumor cell lines. The gene is functionally transcribed in the early stages of embryogenesis and mRNA is also expressed in adult testes. However, in this study, SRY gene transcripts were also detected in somatic cells of adult male and in tumor cells. Moreover, this mRNA possessed a longer additional untranslated exon. Although expression of the transcripts might not have any functional meaning in these cells, these new findings support the hypothesis that any given human cell can contain illegitimate mRNAs.
Kimoto
Y
Y
Department of Surgical Oncology, Biomedical Research Center, Osaka University Medical School, Suita, Japan.
D000328:Adult; D000595:Amino Acid Sequence; D004268:DNA-Binding Proteins / Q000378:metabolism*; D005091:Exons; D006801:Humans; D008214:Lymphocytes / Q000378:metabolism*; D008297:Male; D008969:Molecular Sequence Data; D009687:Nuclear Proteins; D016133:Polymerase Chain Reaction / Q000379:methods; D012333:RNA, Messenger / Q000378:metabolism; D019849:Sex Determination Processes; D051876:Sex-Determining Region Y Protein; D014157:Transcription Factors; D014158:Transcription, Genetic; D014407:Tumor Cells, Cultured
D004268:DNA-Binding Proteins; D009687:Nuclear Proteins; D012333:RNA, Messenger; C497745:SRY protein, human; D051876:Sex-Determining Region Y Protein; D014157:Transcription Factors