title: Expression of two zebrafish homologues of the murine Six3 gene demarcates the initial eye primordia.
abstract: The murine homeobox gene Six3 and its Drosophila homologue sine oculis both have regulatory functions in eye development. We report the isolation and characterization of two zebrafish genes, six3 and six6, that are closely related to the murine Six3 gene. Zebrafish six3 may be the structural orthologue, while the six6 gene is more similar with respect to embryonic expression. Transcripts of both zebrafish six genes are first detected in involuting axial mesendoderm and, subsequently, in the overlying anterior neural plate from which the optic vesicles and the forebrain will develop. Direct correspondence between six3/six6 expression boundaries and the optic vesicles indicate essential roles in defining the eye primordia. During later stages only the six6 gene displays similar features of expression in the eyes and rostral brain as reported previously for murine Six3.
authors.affiliation: Department of Molecular Biology, University of Bergen, HIB-Biobuilding, N-5020, Bergen, Norway.
authors.forename: H C
authors.initials: HC
authors.lastname: Seo
authors.lastname: Drivenes
authors.forename: S
authors.initials: S
authors.lastname: Ellingsen
authors.forename: A
authors.initials: A
authors.lastname: Fjose
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001921:Brain / Q000196:embryology / Q000378:metabolism; D002490:Central Nervous System / Q000196:embryology / Q000378:metabolism; D018076:DNA, Complementary / Q000302:isolation & purification; D005123:Eye / Q000196:embryology* / Q000378:metabolism; D005136:Eye Proteins; D005775:Gastrula / Q000378:metabolism / Q000502:physiology; D018507:Gene Expression Regulation, Developmental; D005801:Genes, Homeobox; D018398:Homeodomain Proteins / Q000096:biosynthesis / Q000235:genetics* / Q000302:isolation & purification; D051379:Mice; D008969:Molecular Sequence Data; D009419:Nerve Tissue Proteins / Q000096:biosynthesis / Q000235:genetics* / Q000302:isolation & purification; D017386:Sequence Homology, Amino Acid; D012689:Sequence Homology, Nucleic Acid; D014158:Transcription, Genetic; D015027:Zebrafish / Q000196:embryology* / Q000235:genetics*; D000097607:Homeobox Protein SIX3
chemical_list: D018076:DNA, Complementary; D005136:Eye Proteins; D018398:Homeodomain Proteins; D009419:Nerve Tissue Proteins; D000097607:Homeobox Protein SIX3