Substance P receptor (NK1) gene expression in synovial tissue in rheumatoid arthritis and osteoarthritis.
The distribution of messenger RNA coding for substance P receptor (NK1) in rheumatoid synovia and the relationship between signal intensity of NK1 mRNA and clinical parameters of disease activity were investigated. The synovia from 10 patients with rheumatoid arthritis (RA) and 7 patients with osteoarthritis was investigated by in situ hybridization histochemistry. In rheumatoid synovia, strong hybridization signal of NK1 mRNA was detected in 80% of the cells in both the lining layer and the interstitial layer. Moreover, the signal intensities of NK1 mRNA positively correlated with serum C-reactive protein levels and radiographic grade of joint destruction. These results provide histochemical evidence that rheumatoid synoviocytes strongly express NK1 gene, and the positive relation of the signal intensity of NK1 mRNA with CRP and radiographic severity suggests that the facilitation of NK1 gene expression in rheumatoid synovium relate the disease progression of RA.
Sakai
K
K
Department of Orthopedic Surgery, Faculty of Medicine, Toyama Medical and Pharmaceutical University, Toyama City, Japan.
Matsuno
H
H
Tsuji
H
H
Tohyama
M
M
D000328:Adult; D000368:Aged; D001172:Arthritis, Rheumatoid / Q000378:metabolism* / Q000473:pathology; D002097:C-Reactive Protein / Q000378:metabolism; D002452:Cell Count; D005260:Female; D015870:Gene Expression; D006651:Histocytochemistry; D006801:Humans; D017403:In Situ Hybridization; D007719:Knee Joint / Q000378:metabolism* / Q000473:pathology; D008297:Male; D008875:Middle Aged; D010003:Osteoarthritis / Q000378:metabolism* / Q000473:pathology; D012333:RNA, Messenger / Q000378:metabolism; D018040:Receptors, Neurokinin-1 / Q000235:genetics / Q000378:metabolism*; D012720:Severity of Illness Index; D013583:Synovial Membrane / Q000378:metabolism* / Q000473:pathology
D012333:RNA, Messenger; D018040:Receptors, Neurokinin-1; D002097:C-Reactive Protein