title: Recombinant human osteogenic protein-1 induces dentin formation: an experimental study in miniature swine.
abstract: It was the aim of the present study to investigate the induction of dentin formation by recombinant human osteogenic protein-1 (hOP-1). In 4 adult miniature pigs a total of 16 teeth with artificially exposed dental pulps were capped with 3 mg of a complex of recombinant hOP-1 in collagen matrix (2.5 micrograms/mg), collagen matrix alone, or calcium hydroxide paste. Teeth were removed in block section after a healing period of 5 weeks. Decalcified sections were processed for light microscopy and histomorphometric analysis. In hOP-1 treated teeth substantial amounts of hard tissue formation (osteodentin and tubular dentin) had consistently led to a complete bridging of the defects. Less dentin formation was seen after calcium hydroxide application. In control defects collagen matrix alone failed to form complete dentin bridges. Recombinant human osteogenic protein-1 in a collagen carrier matrix appeared to be suitable as a bio-active capping agent for surgically exposed dental pulps.
authors.affiliation: Department of Conservative Dentistry, University of Kiel, Dental School, Germany.
authors.forename: S
authors.initials: S
authors.lastname: Jepsen
authors.forename: H K
authors.initials: HK
authors.lastname: Albers
authors.forename: B
authors.initials: B
authors.lastname: Fleiner
authors.forename: M
authors.initials: M
authors.lastname: Tucker
authors.forename: D
authors.initials: D
authors.lastname: Rueger
mesh_terms: D000818:Animals; D055419:Bone Morphogenetic Protein 7; D019485:Bone Morphogenetic Proteins / Q000494:pharmacology*; D002126:Calcium Hydroxide / Q000494:pharmacology; D003094:Collagen / Q000494:pharmacology; D003782:Dental Pulp / Q000187:drug effects / Q000378:metabolism; D003785:Dental Pulp Capping; D003809:Dentin, Secondary / Q000187:drug effects* / Q000254:growth & development; D003810:Dentinogenesis / Q000187:drug effects; D005069:Evaluation Studies as Topic; D006801:Humans; D011994:Recombinant Proteins / Q000494:pharmacology; D013552:Swine; D013556:Swine, Miniature; D016212:Transforming Growth Factor beta; D014945:Wound Healing / Q000187:drug effects
chemical_list: C525819:BMP7 protein, human; D055419:Bone Morphogenetic Protein 7; D019485:Bone Morphogenetic Proteins; D011994:Recombinant Proteins; D016212:Transforming Growth Factor beta; D003094:Collagen; D002126:Calcium Hydroxide