Stimulation of the yeast mitochondrial calcium uniporter by hypotonicity and by ruthenium red.
The Ca2+ uptake by mitochondria from the yeast Endomyces magnusii has earlier been found to be driven by the membrane potential and to be stimulated by spermine. It thus functions in a similar fashion as the animal mitochondrial calcium uniporter. Here, it is shown that the uptake is stimulated, i.e., Ca2+ can be accumulated from lower [Ca2+], under hypotonic conditions. Ruthenium Red, an inhibitor of the animal uniporter, under certain conditions, stimulates the yeast uniporter. The mechanism of the stimulation by hypotonicity and Ruthenium Red is discussed.
Bazhenova
E N
EN
Laboratory of Biological Oxidation, Bach Institute of Biochemistry, Russian Academy of Sciences, 117071 Moscow.
Saris
N E
NE
Zvyagilskaya
R A
RA
D002118:Calcium / Q000378:metabolism; D015220:Calcium Channels; D002135:Calcium-Binding Proteins / Q000378:metabolism*; D017136:Ion Transport / Q000187:drug effects; D008928:Mitochondria / Q000378:metabolism*; D009997:Osmotic Pressure; D012430:Ruthenium Red / Q000494:pharmacology*; D004718:Saccharomycetales / Q000378:metabolism*; D013096:Spermine / Q000494:pharmacology
D015220:Calcium Channels; D002135:Calcium-Binding Proteins; C034703:mitochondrial calcium uniporter; D012430:Ruthenium Red; D013096:Spermine; D002118:Calcium