Expression of insulin-like growth factor-binding protein-1 (IGFBP-1) mRNA in embryos and endometrial stromal cells.
The objective of the present study was to investigate the effects of insulin-like growth factor-binding protein-1 (IGFBP-1) on the interaction between mouse embryos and endometrial stromal cells in co-culture systems. To explore quantitatively the changes in expression of IGFBP-1 mRNA from endometrial stromal cells cultured alone or co-cultured with embryos, a combination of reverse transcription-polymerase chain reaction (RT-PCR) and Southern blotting followed by a densitometric analysis was used. In co-culture systems, development of embryos was significantly improved and the production of IGFBP-1 from endometrial stromal cells was stimulated by the embryos. Quantitative analysis showed that expression of IGFBP-1 mRNA in endometrial stromal cells co-cultured with embryos was higher on days 3,5 and 8 (1.6-, 4.5- and 2.3-fold respectively) than in endometrial stromal cells cultured alone. In addition, intensity of PCR products for IGFBP-1 mRNA in endometrial stromal cells co-cultured with embryos was higher on day 5 than on days 3 and 8. However, the expression of IGFBP-1 mRNA in embryos cultured alone was very low. In conclusion, co-culture of embryos with endometrial stromal cells improved the development of embryos and may be associated with the production of IGFBP-1 by the co-cultured endometrial stromal cells. A combination of RT-PCR and Southern blotting followed by a densitometric analysis appeared to be a sufficiently quantitative method to determine changes in IGFBP-1 mRNA values.
Soong
Y K
YK
Department of Obstetrics and Gynecology, Chang-Gung Memorial Hospital and Medical College, Taipei, Taiwan, ROC.
Wang
H S
HS
Cheng
S Y
SY
Chien
H J
HJ
D000818:Animals; D015139:Blotting, Southern; D002478:Cells, Cultured; D018920:Coculture Techniques; D004622:Embryo, Mammalian / Q000378:metabolism*; D004717:Endometrium / Q000378:metabolism*; D005260:Female; D018970:Insulin-Like Growth Factor Binding Protein 1 / Q000235:genetics*; D051379:Mice; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000378:metabolism*; D017154:Stromal Cells / Q000378:metabolism
D018970:Insulin-Like Growth Factor Binding Protein 1; D012333:RNA, Messenger