Computer-aided video angiometry in isolated rabbit hearts: a new method assessing epicardial coronary selectivity.
The clinical value of coronary vasodilators in antianginal therapy depends on the ratio of their epicardial versus resistance coronary vessel actions. The coronary flow measured in standard isolated heart preparations, however, does not allow any conclusion about the function of epicardial vessels. Thus, we established a new technique assessing the epicardial coronary diameter directly by video angiometry. Pictures from the cardiac surface were taken by a videocamera mounted on a long-distance microscope. The video signal was digitized for computer-aided evaluation. An area of interest (AOI) was laid over the vascular section to be measured. The gray values of the pixels across the epicardial vessel were registered, and a mean curve of distribution was obtained by averaging the gray values from all video lines within the AOI. The inner epicardial coronary diameter resulted from the distance between the points of inflection of this mean curve of distribution. Experiments with NO-vasodilators and adenosine showed that epicardial coronary arteries of isolated perfused rabbit hearts have no appreciable tone. Pretreatment of the hearts with a combination of histamine (10[-6] mol/l), cimetidine (10[-5] mol/l), and adenosine (10[-7] mol/1), however, caused a marked contraction of the conductive vessels. NO-donors selectively dilated epicardial vessels in such pretreated hearts whereas adenosine increased both epicardial coronary diameter and coronary flow, with only a slight tendency toward preferential action on resistance vessels in low concentrations. Simultaneous registration of coronary flow and epicardial coronary diameter in isolated rabbit hearts pretreated with a spasmogenic drug combination (histamine, cimetidine, and adenosine) may be a feasible method assessing epicardial selectivity of coronary vasodilators.
Joseph
G
G
Department of Pharmacology, University of Cologne, Germany.
Strassberger
F
F
Klaus
W
W
D000241:Adenosine / Q000494:pharmacology; D000818:Animals; D002927:Cimetidine; D003198:Computer Simulation; D003326:Coronary Circulation / Q000187:drug effects / Q000502:physiology; D006321:Heart / Q000033:anatomy & histology / Q000187:drug effects*; D006632:Histamine; D066298:In Vitro Techniques; D018715:Microscopy, Video / Q000379:methods*; D009569:Nitric Oxide / Q000378:metabolism; D010496:Pericardium / Q000033:anatomy & histology / Q000187:drug effects*; D011817:Rabbits; D014665:Vasodilator Agents / Q000494:pharmacology
D014665:Vasodilator Agents; D009569:Nitric Oxide; D002927:Cimetidine; D006632:Histamine; D000241:Adenosine