title: The Bradyrhizobium japonicum phoB gene is required for phosphate-limited growth but not for symbiotic nitrogen fixation.
abstract: We identified by cloning and DNA sequence analysis the phosphate regulatory gene phoB of Bradyrhizobium japonicum. The deduced gene product displayed pronounced similarity to the PhoB protein of Sinorhizobium meliloti (71.4% identical amino acids). Escherichia coli (50.2%) and other bacterial species. Insertion of a kanamycin resistance cassette into phoB led to impaired growth of the B. japonicum mutant in media containing approximately 25 microM phosphate or less. A standard plant infection test using wild-type and phoB-defective B. japonicum strains showed that the phoB mutation had no effect on the symbiotic properties of B. japonicum with its soybean host plant.
authors.affiliation: Mikrobiologisches Institut, Eidgenössische Technische Hochschule, Zürich, Switzerland. aminder@micro.biol.ethz.ch
authors.forename: A C
authors.initials: AC
authors.lastname: Minder
authors.forename: F
authors.initials: F
authors.lastname: Narberhaus
authors.forename: H M
authors.initials: HM
authors.lastname: Fischer
authors.forename: H
authors.initials: H
authors.lastname: Hennecke
mesh_terms: D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000235:genetics*; D005798:Genes, Bacterial; D008969:Molecular Sequence Data; D009154:Mutation; D009586:Nitrogen Fixation; D010710:Phosphates / Q000378:metabolism*; D012231:Rhizobium / Q000235:genetics* / Q000502:physiology
chemical_list: D001426:Bacterial Proteins; D010710:Phosphates; C061278:PhoB protein, Bacteria