title: Ultrastructure of the resin-dentin interface following reversible and irreversible rewetting.
abstract: OBJECTIVE
  This study investigated the effect of reversible and irreversible rewetting on the application of One-Step, single-bottle, water-free, acetone-based primer/adhesive.
  
  
  METHODS
  Thirty 1 mm dentin discs prepared from third molars were each conditioned with 10% H3PO4 for 20 seconds and rinsed for 20 seconds. They were randomly divided into five groups: Group 1 (positive control): conditioned dentin bonded visibly moist; Group 2: bonded after drying for 3 seconds; Group 3: drying for 3 seconds and rewetting with distilled water; Group 4 (negative control): drying for 3 seconds, stored in 8% glutaraldehyde for 15 minutes, rinsed with distilled water and dried for 3 seconds; and Group 5: drying for 3 seconds, stored in glutaraldehyde for 15 minutes, rinsed with distilled water and bonded visibly moist. One-Step was applied in two coats according to the manufacturer's recommendation and discs in each group were further bonded together to form a disc-pair using a chemical cure resin, demineralized in EDTA and prepared for TEM examination.
  
  
  RESULTS
  Complete resin infiltration into the zone of demineralized dentin was observed in Group 1 and 3. In Group 2, resin infiltration was limited to the surface and base of the demineralized network apparently along the lateral branches of the dentin tubules. A middle zone of suboptimal infiltration was observed. In Group 4 and 5, minimal resin infiltration was only observed along the base of the demineralized network upon their fixation in a collapsed state with glutaraldehyde.
authors.affiliation: Department of Children's Dentistry and Orthodontics, Prince Philip Dental Hospital, University of Hong Kong.
authors.forename: F R
authors.initials: FR
authors.lastname: Tay
authors.forename: A J
authors.initials: AJ
authors.lastname: Gwinnett
authors.forename: S H
authors.initials: SH
authors.lastname: Wei
mesh_terms: D003094:Collagen / Q000737:chemistry*; D001840:Dental Bonding / Q000379:methods*; D003804:Dentin / Q000648:ultrastructure; D003806:Dentin Permeability; D017220:Dentin-Bonding Agents / Q000737:chemistry; D006801:Humans; D008689:Methacrylates / Q000737:chemistry; D008854:Microscopy, Electron; D013499:Surface Properties; D014867:Water / Q000737:chemistry
chemical_list: C103506:Bis-GMA, BPDM, HEMA dental-bonding resin; D017220:Dentin-Bonding Agents; D008689:Methacrylates; D014867:Water; D003094:Collagen