Further assignment of resonances in 1H NMR spectra of cerebrospinal fluid (CSF).
A number of previously unidentified 1H NMR signals detected in CSF spectra of patients with various neurological and metabolic diseases are assigned to metabolites, drugs and drug excipients. Two-dimensional 1H NMR spectroscopy (COSY and J-resolved) is employed to resolve resonances which are hidden by superimposed peaks in one-dimensional spectra. Assignments obtained by making use of 2-D techniques, and of a 1-D 1H NMR data base created for ca. 150 authentic compounds, enable us to clarify the nature of complex signal patterns found in crowded spectral regions of CSF such as the aliphatic methyl region at ca. 1.0 ppm.
Lutz
N W
NW
Centre de Résonance Magnétique Biologique et Médicale, UMR CNRS 6612, Faculté de Médecine, Marseille, France.
Maillet
S
S
Nicoli
F
F
Viout
P
P
Cozzone
P J
PJ
D000438:Alcohols / Q000134:cerebrospinal fluid; D002555:Cerebrospinal Fluid / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D005227:Fatty Acids / Q000134:cerebrospinal fluid; D006801:Humans; D009536:Niacinamide / Q000134:cerebrospinal fluid; D019906:Nuclear Magnetic Resonance, Biomolecular; D010889:Piracetam / Q000134:cerebrospinal fluid; D011522:Protons; D012815:Signal Processing, Computer-Assisted
D000438:Alcohols; D005227:Fatty Acids; D011522:Protons; D009536:Niacinamide; D010889:Piracetam