title: [Quantification of regional cerebral blood flow at the control state and loaded with diamox using split-dose 99mTc-ECD SPECT and venous blood samples].
abstract: The purpose of the study is to develop a simple and less invasive method for quantifying regional cerebral blood flow (rCBF) at pre- and post Diamox test using split-dose 99mTc-ECD and SPECT. By employing a microsphere model, integral of input function was calculated by the one-point venous sampling method previously reported. The study was performed on 5 subjects with cerebrovascular diseases. A split dose of 99mTc-ECD was injected pre- and post Diamox injection, and rCBF was measured by two SPECT scans and single venous samples, respectively. Mean CBF obtained by the present method was 0.47 +/- 0.07 ml/g/min at the control state, and 0.63 +/- 0.12 ml/g/min loaded with Diamox (mean % increase; 35%), showing good agreement with those obtained by the 133Xe-inhalation method. Since the present method does not require arterial blood sampling, dynamic data acquisition and dose corrections, it is simple, less invasive and useful in clinical SPECT studies.
authors.affiliation: Department of Radiology, Niigata University School of Medicine.
authors.forename: I
authors.initials: I
authors.lastname: Odano
authors.forename: M
authors.initials: M
authors.lastname: Ohkubo
authors.forename: E
authors.initials: E
authors.lastname: Noguchi
authors.forename: H
authors.initials: H
authors.lastname: Ohtaki
authors.forename: M
authors.initials: M
authors.lastname: Shibaki
authors.forename: S
authors.initials: S
authors.lastname: Dobashi
mesh_terms: D000086:Acetazolamide; D000328:Adult; D000368:Aged; D001921:Brain / Q000000981:diagnostic imaging*; D002560:Cerebrovascular Circulation; D002561:Cerebrovascular Disorders / Q000000981:diagnostic imaging* / Q000503:physiopathology; D003545:Cysteine / Q000031:analogs & derivatives*; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D015609:Organotechnetium Compounds; D019275:Radiopharmaceuticals; D015899:Tomography, Emission-Computed, Single-Photon / Q000379:methods*
chemical_list: D015609:Organotechnetium Compounds; D019275:Radiopharmaceuticals; C059659:technetium Tc 99m bicisate; D003545:Cysteine; D000086:Acetazolamide