Okadaic acid induces appearance of the mitotic epitope MPM-2 in SV40-infected CV-1 cells with a >G2-phase DNA content.
Simian virus 40 (SV40) infection of quiescent monkey kidney cells stimulates two successive rounds of cellular DNA synthesis without an intervening mitosis. This uncoupling of S phase and mitosis indicates that SV40 modulates pathways regulating the G2-to-M phase transition. To examine the integrity of mitotic initiation pathways in infected cells that have bypassed mitosis, SV40-infected CV-1 cells were treated with okadaic acid (OA), a known inducer of premature mitosis in other cell types. OA treatment triggered the appearance of the mitotic marker MPM-2 in SV40-infected CV-1 cells progressing through either the first (diploid) or second (tetraploid) S phases. These results demonstrate that a subset of mitotic pathways are intact but inactive in SV40-infected cells that have bypassed mitosis and initiated tetraploid S phase.
Friedrich
T D
TD
Department of Microbiology, Immunology and Molecular Genetics, Albany Medical College, New York 12208, USA. tom_friedrich@ccgateway.amc.edu
Okubo
E
E
Laffin
J
J
Lehman
J M
JM
D000818:Animals; D002460:Cell Line; D002522:Chlorocebus aethiops; D004247:DNA / Q000235:genetics / Q000378:metabolism*; D000939:Epitopes / Q000096:biosynthesis; D005434:Flow Cytometry; D016195:G2 Phase / Q000187:drug effects*; D008938:Mitosis / Q000187:drug effects; D019319:Okadaic Acid / Q000494:pharmacology*; D010750:Phosphoproteins / Q000096:biosynthesis* / Q000276:immunology; D011123:Polyploidy; D013539:Simian virus 40 / Q000472:pathogenicity / Q000502:physiology
D000939:Epitopes; D010750:Phosphoproteins; D019319:Okadaic Acid; D004247:DNA