title: Highly sensitive and specific assay of plasma melatonin using high-performance liquid chromatography with fluorescence detection preceded by solid-phase extraction.
abstract: This paper describes a new, highly sensitive, rapid method for the determination of low levels of endogenous melatonin, the pineal and retinal hormone, in fish plasma samples. The combination of solid-phase extraction and high-performance liquid chromatography with fluorescence detection significantly improves the separation and increases the sensitivity of the assay. The proposed method may be a useful alternative to previously reported procedures. The assay should be applicable to plasma and tissue samples from other animals, including humans, with only minor modifications.
authors.affiliation: Marine Biology Center of Polish Academy of Sciences, Gdynia, Poland.
authors.forename: E
authors.initials: E
authors.lastname: Kulczykowska
authors.forename: P M
authors.initials: PM
authors.lastname: Iuvone
mesh_terms: D000818:Animals; D002851:Chromatography, High Pressure Liquid; D005260:Female; D005432:Flounder / Q000378:metabolism*; D008297:Male; D008550:Melatonin / Q000097:blood*; D013050:Spectrometry, Fluorescence
chemical_list: D008550:Melatonin