Secretion and increase of intracellular CuZn superoxide dismutase content in human neuroblastoma SK-N-BE cells subjected to oxidative stress.
CuZn superoxide dismutase (SOD) secretion was detected in media of [35S]cysteine-labeled human neuroblastoma SK-N-BE cells precipitated with antihuman CuZn SOD antibodies. The ability of Fe2+/ascorbate oxidative stress to induce CuZn SOD in SK-N-BE cells was evaluated by Western blot analysis. The results showed that, like human hepatocarcinoma cells and human fibroblasts, SK-N-BE cells secrete CuZn SOD. In addition, the CuZn SOD concentration was higher in cells subjected to oxidative stress than in unstressed cells. The secretion of CuZn SOD and the ability of Fe2+/ascorbate to increase its protein content in SK-N-BE cells indicates that this enzyme protects the brain from damage induced by oxidative stress.
Mondola
P
P
Dipartimento di Neuroscienze e della Communicazione Interumana, Sezione Fisiologia, Università degli Studi di Napoli Federico II, Naples, Italy. mondola@unina.it
Annella
T
T
Serù
R
R
Santangelo
F
F
Iossa
S
S
Gioielli
A
A
Santillo
M
M
D001205:Ascorbic Acid / Q000494:pharmacology; D005296:Ferrous Compounds / Q000494:pharmacology; D005609:Free Radicals / Q000378:metabolism; D006801:Humans; D007700:Kinetics; D007770:L-Lactate Dehydrogenase / Q000032:analysis; D008315:Malondialdehyde / Q000378:metabolism; D009447:Neuroblastoma / Q000201:enzymology*; D018384:Oxidative Stress / Q000502:physiology*; D013482:Superoxide Dismutase / Q000096:biosynthesis* / Q000378:metabolism; D017392:Thiobarbituric Acid Reactive Substances / Q000032:analysis; D014407:Tumor Cells, Cultured
D005296:Ferrous Compounds; D005609:Free Radicals; D017392:Thiobarbituric Acid Reactive Substances; C051140:iron(II)-ascorbic acid complex; D008315:Malondialdehyde; D007770:L-Lactate Dehydrogenase; D013482:Superoxide Dismutase; D001205:Ascorbic Acid