Semicarbazide-sensitive amine oxidase in pig heart.
A semicarbazide-sensitive amine oxidase (SSAO) (E.C.1.4.3.6) has been purified from pig heart. Western blot analysis showed that the enzyme reacts with a polyclonal antibody raised against homogeneous crystalline pig plasma benzylamine oxidase (BAO). A subunit molecular mass of 97 KDa obtained by SDS electrophoresis is identical to the plasma enzyme. The purification procedure consisted of sequential DEAE cellulose, octyl-Sepharose, Con A-Sepharose and hydroxyapatite columns. Two peaks of activity were obtained on octyl-Sepharose which were found to be kinetically and immunologically indistinguishable. The specific activity of the purified enzyme was 0.045 mumol/min/mg of protein at 37 degrees C and the Km for benzylamine was estimated to be 63 microM. The enzyme was inhibited by carbonyl reagents such as semicarbazide but was insensitive to the effect of pargyline.
Dowling
T G
TG
Department of Pharmacology, University of Florence, Italy.
Cambi
S
S
Buffoni
F
F
D006631:Amine Oxidase (Copper-Containing) / Q000276:immunology / Q000302:isolation & purification / Q000378:metabolism*; D000818:Animals; D000906:Antibodies; D001595:Benzylamine Oxidase / Q000276:immunology / Q000302:isolation & purification; D002845:Chromatography; D002846:Chromatography, Affinity; D002848:Chromatography, DEAE-Cellulose; D002852:Chromatography, Ion Exchange; D017886:Durapatite; D007700:Kinetics; D009206:Myocardium / Q000201:enzymology*; D013552:Swine
D000906:Antibodies; D017886:Durapatite; D001595:Benzylamine Oxidase; D006631:Amine Oxidase (Copper-Containing)