title: Systemic complement depletion reduces inflammation and demyelination in adoptive transfer experimental allergic neuritis.
abstract: The effect of systemic complement depletion by cobra venom factor (CVF) was evaluated in adoptive transfer experimental allergic neuritis (AT-EAN). Spleen cells of rats immunized with a neuritogenic peptide SP26 were injected into naive rats. On days 3 and 6 after cell transfer AT-EAN rats were treated with CVF or saline intraperitoneally. AT-EAN rats treated with CVF had significantly lower scores for histological inflammation (0.25 +/- 0.25 vs 1.9 +/- 0.4, mean +/- SEM, P < 0.03) and demyelination (0.13 +/- 0.13 vs 1.6 +/- 1.4, P < 0.02) than saline-treated AT-EAN rats. Immunocytochemistry of lumbosacral nerve roots showed significantly less ED1-positive macrophages (0.5 +/- 0.3 vs 1.6 +/- 0.6, P < 0.04) and CD11bc-positive (expressing complement receptor 3 or CR3) inflammatory cells (0.6 +/- 0.4 vs 1.7 +/- 0.5, P < 0.03). Our data suggest that complement plays a crucial role in inflammatory demyelination since systemic complement depletion significantly reduces recruitment of macrophages into the nerve and subsequent macrophage-mediated demyelination.
authors.affiliation: Department of Neurology, University of Texas Health Science Center, Houston 77030, USA. fvriesen@neuro.med.uth.tmc.edu
authors.forename: F J
authors.initials: FJ
authors.lastname: Vriesendorp
authors.forename: R E
authors.initials: RE
authors.lastname: Flynn
authors.forename: M R
authors.initials: MR
authors.lastname: Malone
authors.forename: M A
authors.initials: MA
authors.lastname: Pappolla
mesh_terms: D000818:Animals; D018845:CD11 Antigens / Q000032:analysis; D002420:Cauda Equina / Q000737:chemistry / Q000473:pathology; D003169:Complement Inactivator Proteins; D003165:Complement System Proteins / Q000276:immunology / Q000378:metabolism*; D003711:Demyelinating Diseases / Q000276:immunology / Q000378:metabolism* / Q000473:pathology; D003713:Dendritic Cells / Q000737:chemistry / Q000276:immunology; D004546:Elapid Venoms; D005260:Female; D007150:Immunohistochemistry; D008264:Macrophages / Q000737:chemistry / Q000276:immunology; D009444:Neuritis, Autoimmune, Experimental / Q000276:immunology / Q000378:metabolism* / Q000473:pathology; D051381:Rats; D011917:Rats, Inbred Lew; D013126:Spinal Nerve Roots / Q000737:chemistry / Q000473:pathology
chemical_list: D018845:CD11 Antigens; D003169:Complement Inactivator Proteins; D004546:Elapid Venoms; C016310:cobra venom factor; D003165:Complement System Proteins