Molecular cloning of the cDNA encoding the constant region of the immunoglobulin A heavy chain (C alpha) from a marsupial: Trichosurus vulpecula (common brushtail possum)
A cDNA encoding the brushtail possum immunoglobulin A heavy chain constant region (C alpha) was isolated by screening a mesenteric lymph node cDNA library with a porcine C alpha exon 3 probe. The larger of the two positive clones isolated (Tv4a) consisted of 1325 bp of possum cDNA that included an open reading frame of 1191 bp. Its deduced amino acid sequence had a high degree of sequence identity with known eutherian C alpha sequences. This clone appears to encode the entire possum IgA heavy chain constant region. The possum C alpha sequence had a nucleotide sequence identity of 57.7% with porcine C alpha, 51% with mouse C alpha, 46.7% with dog C alpha and 45.9% with human C alpha2. The corresponding amino acid identities were 46.7, 45.6, 49.4 and 49%, respectively.
Belov
K
K
CRC for Conservation and Management of Marsupials, School of Biological Sciences, Macquarie University, North Ryde, NSW, Australia.
Harrison
G A
GA
Cooper
D W
DW
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000737:chemistry; D015723:Gene Library; D006801:Humans; D007070:Immunoglobulin A / Q000235:genetics*; D007127:Immunoglobulin Constant Regions / Q000235:genetics*; D007143:Immunoglobulin Heavy Chains / Q000235:genetics*; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D009893:Opossums / Q000235:genetics* / Q000276:immunology*; D016415:Sequence Alignment
D018076:DNA, Complementary; D007070:Immunoglobulin A; D007127:Immunoglobulin Constant Regions; D007143:Immunoglobulin Heavy Chains