Human recombinant Puumala virus antibodies: cross-reaction with other hantaviruses and use in diagnostics.
A panel of seven human monoclonal Fabs against Puumala virus (PUU) nucleocapsid protein (N) was obtained by panning an antibody phage-display library prepared from the spleen of a PUU-immune individual. Three antibodies reacted in immunoblotting and cross-reacted strongly with Tula and Sin Nombre virus recombinant N proteins. These antibodies mapped to the amino terminus of the N protein. One PUU glycoprotein 2 (G2)-specific Fab obtained against a novel epitope (G2c) cross-reacted with Khabarovsk virus but not with the other hantavirus serotypes. An N protein-specific Fab was successfully used as capture antibody to detect PUU-specific serum IgG and IgM antibodies in an enzyme immunoassay. The result demonstrates the usefulness of recombinant human Fabs as potential diagnostic tools.
Salonen
E M
EM
Department of Immunology, The Scripps Research Institute, La Jolla, CA 92037, USA.
Parren
P W
PW
Graus
Y F
YF
Lundkvist
A
A
Fisicaro
P
P
Vapalahti
O
O
Kallio-Kokko
H
H
Vaheri
A
A
Burton
D R
DR
D000911:Antibodies, Monoclonal / Q000235:genetics; D000914:Antibodies, Viral / Q000235:genetics; D003429:Cross Reactions; D004247:DNA / Q000235:genetics; D018604:Epitope Mapping; D006476:Orthohantavirus / Q000145:classification / Q000235:genetics / Q000276:immunology*; D018778:Hantavirus Infections / Q000175:diagnosis / Q000276:immunology / Q000821:virology; D006801:Humans; D007140:Immunoglobulin Fab Fragments / Q000235:genetics; D007074:Immunoglobulin G / Q000097:blood; D007075:Immunoglobulin M / Q000097:blood; D008969:Molecular Sequence Data; D019251:Nucleocapsid / Q000235:genetics / Q000276:immunology*; D019590:Nucleocapsid Proteins; D011994:Recombinant Proteins / Q000235:genetics / Q000276:immunology
D000911:Antibodies, Monoclonal; D000914:Antibodies, Viral; D007140:Immunoglobulin Fab Fragments; D007074:Immunoglobulin G; D007075:Immunoglobulin M; D019590:Nucleocapsid Proteins; D011994:Recombinant Proteins; C100886:nucleocapsid protein, Puumala virus; D004247:DNA