Relief of transcriptional polarity by a mutation that creates a promoter in the hisG gene of Salmonella typhimurium LT2.
Six mutant strains were independently isolated in Salmonella typhimurium as non-polar revertants of a polar Tn10 insertion in hisG. DNA sequence analysis showed that all six mutants result from the same nucleotide alteration: a G/C to A/T transition 4 bp from the end of the hisG coding sequence. We present data suggesting that the mutation (hisG10225) acts by creating a new transcriptional initiation signal. Furthermore, the hisG10225 mutation renders the hisG gene product cold sensitive.
Bianco
R
R
Istituto di Genetica, Università di Bari, Italy.
Moramarco
G
G
Stella
A
A
Ciampi
M S
MS
D001426:Bacterial Proteins; D001483:Base Sequence; D004251:DNA Transposable Elements; D015870:Gene Expression; D005798:Genes, Bacterial; D008969:Molecular Sequence Data; D009004:Monosaccharide Transport Proteins / Q000235:genetics*; D016254:Mutagenesis, Insertional; D009154:Mutation; D009876:Operon; D011401:Promoter Regions, Genetic; D012486:Salmonella typhimurium / Q000235:genetics*; D017422:Sequence Analysis, DNA
D001426:Bacterial Proteins; D004251:DNA Transposable Elements; C106638:HisG protein, bacteria; D009004:Monosaccharide Transport Proteins