Expression of human estrogen sulfotransferase in Salmonella typhimurium: differences between hHST and hEST in the enantioselective activation of 1-hydroxyethylpyrene to a mutagen.
Various human sulfotransferases (hP-PST, hM-PST, hHST) and rat sulfotransferases (rPST-IV, rHSTa) have already been expressed in Ames' Salmonella strains (in particular in TA1538). Now a further strain, TA1538-hEST, which expresses the human estrogen sulfotransferase (hEST), has been constructed. This strain activated the primary benzylic alcohol 1-hydroxymethylpyrene (1-HMP) and the secondary benzylic alcohol 1-hydroxyethylpyrene (1-HEP) to mutagens. Human sulfotransferases hEST and hHST both activated 1-HEP, but they differed substantially in their enantioselectivity for this compound.
Hagen
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M
German Institute of Human Nutrition, Potsdam-Rehbrücke.
Pabel
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U
Landsiedel
R
R
Bartsch
I
I
Falany
C N
CN
Glatt
H
H
D000818:Animals; D001592:Benzyl Alcohols / Q000493:pharmacokinetics / Q000633:toxicity; D015153:Blotting, Western; D003600:Cytosol / Q000201:enzymology; D006801:Humans; D009152:Mutagenicity Tests; D009153:Mutagens / Q000493:pharmacokinetics* / Q000633:toxicity; D051381:Rats; D012486:Salmonella typhimurium / Q000187:drug effects / Q000201:enzymology* / Q000235:genetics; D013237:Stereoisomerism; D013379:Substrate Specificity; D015238:Sulfotransferases / Q000096:biosynthesis / Q000235:genetics / Q000378:metabolism*
D001592:Benzyl Alcohols; D009153:Mutagens; D015238:Sulfotransferases; C028152:alcohol sulfotransferase; C020122:estrone sulfotransferase