Stat3 activation is required for cellular transformation by v-src.
Stat3 activation has been associated with cytokine-induced proliferation, anti-apoptosis, and transformation. Constitutively activated Stat3 has been found in many human tumors as well as v-abl- and v-src-transformed cell lines. Because of these correlations, we examined directly the relationship of activated Stat3 to cellular transformation and found that wild-type Stat3 enhances the transforming potential of v-src while three dominant negative Stat3 mutants inhibit v-src transformation. Stat3 wild-type or mutant proteins did not affect v-ras transformation. We conclude that Stat3 has a necessary role in v-src transformation.
Bromberg
J F
JF
Laboratory of Molecular Cell Biology, The Rockefeller University, New York, New York 10021-6399, USA.
Horvath
C M
CM
Besser
D
D
Lathem
W W
WW
Darnell
J E
JE
D016475:3T3 Cells; D000818:Animals; D002470:Cell Survival; D002471:Cell Transformation, Neoplastic; D004268:DNA-Binding Proteins / Q000235:genetics / Q000378:metabolism*; D015972:Gene Expression Regulation, Neoplastic; D051379:Mice; D015688:Oncogene Protein pp60(v-src) / Q000378:metabolism*; D050796:STAT3 Transcription Factor; D013234:Stem Cells; D015534:Trans-Activators / Q000235:genetics / Q000378:metabolism*
D004268:DNA-Binding Proteins; D050796:STAT3 Transcription Factor; C494087:Stat3 protein, mouse; D015534:Trans-Activators; D015688:Oncogene Protein pp60(v-src)