Cloning and expression pattern of Xenopus prx-1 (Xprx-1) during embryonic development.
Homeobox genes are expressed both temporally and spatially during vertebrate development, and regulate the tissue-specific expression of other genes. A Xenopus paired-related homeobox- 1 (Xprx-1) cDNA was cloned. Xprx-1 had a paired-related homeodomain, but did not contain a paired-box. The sequence of Xprx-1 had a high level of homology with K-2(mouse) and Prx-1 (chicken), thus Xprx-1 is assumed to be the Xenopus homolog of these genes. Xprx-1 transcripts were maternally restricted, in Xenopus embryos, and a decrease in the late blastula stage was followed by an increase in zygotic transcripts after gastrulation. The transcripts were localized to the animal hemisphere of the late blastula and were concentrated in the branchial arches of the tail-bud stage embryo. In animal cap experiments, Activin A dose-dependently induced Xprx-1 gene expression. These results suggest that Xprx-1 plays a role in early Xenopus development similar to other species.
Takahashi
S
S
Department of Life Science (Biology), Japan Science and Technology Corporation, The University of Tokyo, Meguro, Japan.
Uochi
T
T
Kawakami
Y
Y
Nohno
T
T
Yokota
C
C
Kinoshita
K
K
Asashima
M
M
D028341:Activins; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D001755:Blastocyst / Q000737:chemistry; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics; D004268:DNA-Binding Proteins / Q000235:genetics*; D004475:Ectoderm; D004627:Embryonic Induction; D016222:Fibroblast Growth Factor 2 / Q000494:pharmacology; D005775:Gastrula / Q000737:chemistry; D018507:Gene Expression Regulation, Developmental / Q000502:physiology*; D005801:Genes, Homeobox / Q000235:genetics; D018398:Homeodomain Proteins; D007265:Inhibins / Q000494:pharmacology; D008969:Molecular Sequence Data; D012333:RNA, Messenger / Q000032:analysis; D017386:Sequence Homology, Amino Acid; D014157:Transcription Factors / Q000235:genetics*; D029867:Xenopus Proteins; D014982:Xenopus laevis
D018076:DNA, Complementary; D004268:DNA-Binding Proteins; D018398:Homeodomain Proteins; C489937:PRRX1 protein, Xenopus; C489936:Prrx1 protein, mouse; D012333:RNA, Messenger; D014157:Transcription Factors; D029867:Xenopus Proteins; D016222:Fibroblast Growth Factor 2; D028341:Activins; D007265:Inhibins