Hexokinase PII has a double cytosolic-nuclear localisation in Saccharomyces cerevisiae.
We describe here that the HXK2 gene product, isoenzyme PII of hexokinase, is located in both the nucleus and the cytoplasm of Saccharomyces cerevisiae cells. This conclusion is supported by assays of hexokinase-specific activity in isolated nuclei from wild-type and hxk1lhxk2 double mutant strains, by immunoblot experiments using anti-Hxk2 antibodies and by observation of the fluorescence distribution of a Hxk2-GFP fusion protein in cells transformed with the HXK2::gfp gene.
Randez-Gil
F
F
Departamento de Biotecnología de Alimentos, Instituto de Agroquímica y Tecnología de Alimentos, CSIC, Burjassot Valencia, Spain.
Herrero
P
P
Sanz
P
P
Prieto
J A
JA
Moreno
F
F
D002458:Cell Fractionation; D002467:Cell Nucleus / Q000201:enzymology; D003593:Cytoplasm / Q000201:enzymology; D004794:Enzyme Repression / Q000502:physiology; D005455:Fluorescent Antibody Technique; D005656:Fungal Proteins / Q000378:metabolism; D005947:Glucose / Q000378:metabolism; D006593:Hexokinase / Q000378:metabolism*; D007527:Isoenzymes / Q000378:metabolism; D009154:Mutation / Q000235:genetics; D010766:Phosphorylation; D011523:Protoplasts / Q000201:enzymology; D011994:Recombinant Proteins / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000201:enzymology* / Q000235:genetics
D005656:Fungal Proteins; D007527:Isoenzymes; D011994:Recombinant Proteins; D006593:Hexokinase; D005947:Glucose