title: Molecular heterogeneity of Vicia villosa-recognized perineuronal nets surrounding pyramidal and nonpyramidal neurons in the guinea pig cerebral cortex.
abstract: Of three monoclonal antibodies (mAbs Cat 301, 1B5 and 473) which recognize epitopes on perineuronal nets (PnNs), only mAb 473 displayed considerably varied degrees of staining intensity on Vicia villosa-labeled pyramidal (P) neurons (5%, intensely; 54%, very weakly; and 41%, unstained). These results indicate the heterogeneity on molecular structure or composition of the terminal N-acetylgalactosamine-containing PnNs within P class as well as between P and nonpyramidal classes.
authors.affiliation: 1st Department of Anatomy, Toho University School of Medicine, 5-21-16, Omori-Nishi, Ota-ku, Tokyo 143, Japan. yojima@med.toho-u.ac.jp
authors.forename: H
authors.initials: H
authors.lastname: Ojima
authors.forename: M
authors.initials: M
authors.lastname: Sakai
authors.forename: J
authors.initials: J
authors.lastname: Ohyama
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal; D002540:Cerebral Cortex / Q000166:cytology / Q000378:metabolism*; D005260:Female; D006168:Guinea Pigs; D006651:Histocytochemistry; D007150:Immunohistochemistry; D037102:Lectins; D008297:Male; D008856:Microscopy, Fluorescence; D017350:N-Acetylgalactosaminyltransferases / Q000378:metabolism; D009415:Nerve Net / Q000502:physiology*; D009474:Neurons / Q000378:metabolism; D037121:Plant Lectins; D017966:Pyramidal Cells / Q000378:metabolism*; D000097763:Polypeptide N-acetylgalactosaminyltransferase
chemical_list: D000911:Antibodies, Monoclonal; D037102:Lectins; D017350:N-Acetylgalactosaminyltransferases; D037121:Plant Lectins; D000097763:Polypeptide N-acetylgalactosaminyltransferase; C042242:Vicia lectins; C023220:(N-acetylneuraminyl)-galactosylglucosylceramide N-acetylgalactosaminyltransferase