A method for hormonal induction of sperm release in anurans (eight species) and in vitro fertilization in lepidobatrachus species.
Injections of synthetic human gonadotropin releasing hormone (GnRH) into the dorsal pelvic area were used in an attempt to stimulate sperm release in isolated males of eight anuran species including Xenopus laevis, Rana pipiens and Lepidobatrachus laevis. Sperm were obtained within 1-5 h post injection either by mechanical stimulation or by cloacal lavage. Sperm suspensions varied from 8 microL to 7 mL and the cell densities ranged from 4 x 10(5) to 4 x 10(7) sperm/mL. The sperm obtained from seven species using GnRH-induced release were viable based on light microscopic observations of motility. In addition, sperm preparations fertilized eggs in vitro and produced normal tadpoles in the case of L. laevis and L. Ilanensis. This hormonal method of anuran sperm collection will provide a convenient non-injurious way to obtain anuran sperm for basic studies of reproduction and development.
Waggener
W L
WL
Department of Biology, University of California, Riverside 92521, USA.
Carroll
E J
EJ
D000818:Animals; D001001:Anura / Q000502:physiology*; D005307:Fertilization in Vitro / Q000379:methods*; D007987:Gonadotropin-Releasing Hormone / Q000494:pharmacology*; D006801:Humans; D008297:Male; D013079:Sperm Maturation / Q000187:drug effects*; D013081:Sperm Motility; D013094:Spermatozoa / Q000502:physiology*
D007987:Gonadotropin-Releasing Hormone