Determination of propofol in low-volume samples by high-performance liquid chromatography with fluorescence detection.
In order to determine propofol in rat whole-blood samples of 50 microl, we developed a rapid, simple and reliable method which is characterized by precipitation of blood elements with acetonitrile and submission of the supernatant to HPLC analysis with fluorescence detection. The method described is linear from 0.4 to 40 mg/l and the relative standard deviations in this concentration range are less than 10%. The limit of quantification proved to be 0.4 mg/l. Blood constituents do not interfere with the assay.
Knibbe
C A
CA
Department of Clinical Pharmacy, St. Anonius Hospital, Nieuwegein, The Netherlands.
Koster
V S
VS
Deneer
V H
VH
Stuurman
R M
RM
Kuks
P F
PF
Lange
R
R
D018686:Anesthetics, Intravenous / Q000008:administration & dosage / Q000097:blood* / Q000493:pharmacokinetics; D000818:Animals; D002138:Calibration; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D002940:Circadian Rhythm; D007262:Infusions, Intravenous; D016014:Linear Models; D015742:Propofol / Q000008:administration & dosage / Q000097:blood* / Q000493:pharmacokinetics; D051381:Rats; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013050:Spectrometry, Fluorescence
D018686:Anesthetics, Intravenous; D015742:Propofol