Localization of protein kinase C alpha and zeta during the decidualization in pseudopregnant rats.
Our previous data showed that at least five PKC isoforms (alpha, delta, zeta, lambda and tau) were present in the decidualization. In this study, we then localized the PKC alpha and zeta by immunohistochemistry in the decidualized uterine tissues. The decidualized uterine tissues were induced by trauma-stimulation and fixed in formalin. The immunofluorescence were photographed by confocal microscope. The data revealed that the fluorescence of PKC alpha was present in the deciduomata and myometrium. In the deciduomata, PKC alpha was mainly located in the surrounding nuclear. This phenomenon of localization was especially performed on day 2 and 3 of the decidualization, just on the time of higher frequence of cell mitosis. Since the myometrium with hypertrophy did not display the phenomenon of perinuclear localization, these suggested that the expression and localization of PKC alpha may be associated with the cell proliferation. On the other hand, the PKC zeta was also present and distributed broadly in the deciduomata and myometrium. This expression was increased and similar to the previous Western blot studies. Thus, the data confirmed that the various expression and localization of PKC isoforms may be correlated with the development of deciduomata.
Shyu
J C
JC
Department of Physiology, Institute of Biochemistry, Taiwan, ROC.
Hsieh
Y S
YS
Chang
C L
CL
Tsai
C C
CC
Chang
A C
AC
Yang
L C
LC
Lin
M T
MT
Cheng
M H
MH
Liu
J Y
JY
D000818:Animals; D003656:Decidua / Q000201:enzymology*; D005260:Female; D007527:Isoenzymes / Q000378:metabolism*; D011493:Protein Kinase C / Q000378:metabolism*; D011555:Pseudopregnancy / Q000201:enzymology*; D051381:Rats; D017207:Rats, Sprague-Dawley
D007527:Isoenzymes; D011493:Protein Kinase C