In vivo regulation of the entry into M-phase: initial activation and nuclear translocation of cyclin B/Cdc2.
The cyclin B/Cdc2 complex, Cdc2 kinase governs M-phase. Although the intracomplex modification for its activation in vitro has been described extensively, its regulation in vivo is not so well explained so far. In this article, we will focus on the intracellular regulation of the cyclin B/Cdc2 activity, in particular, how it is initially activated in vivo, how its nuclear translocation is executed specifically at the onset of M-phase, and how the activation and the nuclear translocation are coordinated in the cell. These concerted regulations may determine the appropriate timing for the initiation of M-phase.
Kishimoto
T
T
Laboratory of Cell and Developmental Biology, Faculty of Biosciences and Biotechnology, Tokyo Institute of Technology, Yokohama, Japan.
Okumura
E
E
D000818:Animals; D001693:Biological Transport, Active; D016203:CDC2 Protein Kinase / Q000378:metabolism*; D002467:Cell Nucleus / Q000378:metabolism; D019926:Cyclin B / Q000378:metabolism*; D003593:Cytoplasm / Q000378:metabolism; D004789:Enzyme Activation; D005246:Feedback; D006801:Humans; D007399:Interphase / Q000502:physiology; D008938:Mitosis / Q000502:physiology*; D008954:Models, Biological
D019926:Cyclin B; D016203:CDC2 Protein Kinase