title: Activity staining on polyacrylamide gels of trypsin inhibitors from leaves of sweet potato (Ipomoea batatas L. Lam) varieties.
abstract: The failure of activity staining of trypsin inhibitors in crude leaf extracts of sweet potato varieties including Tainong 27, Tainong 34, and Tainong 57 on a 15% sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) gel was prevented by dipping the gels in solutions containing 10-40 mM hydrogen peroxide, 10 mM Tris buffer (pH 7.9) for 30 min before staining.
authors.affiliation: Institute of Botany, Academia Sinica, Nankang, Taipei, Taiwan, R.O.C.
authors.forename: W C
authors.initials: WC
authors.lastname: Hou
authors.forename: Y H
authors.initials: YH
authors.lastname: Lin
mesh_terms: D000180:Acrylic Resins; D003683:Dehydroascorbic Acid; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods*; D004791:Enzyme Inhibitors / Q000032:analysis*; D005978:Glutathione; D006861:Hydrogen Peroxide; D018515:Plant Leaves / Q000737:chemistry; D010940:Plant Proteins / Q000032:analysis*; D019657:Solanaceae / Q000737:chemistry*; D013194:Staining and Labeling / Q000379:methods*; D014361:Trypsin Inhibitors; D000516:alpha-Amylases / Q000037:antagonists & inhibitors
chemical_list: D000180:Acrylic Resins; D004791:Enzyme Inhibitors; D010940:Plant Proteins; D014361:Trypsin Inhibitors; C016680:polyacrylamide gels; D006861:Hydrogen Peroxide; D000516:alpha-Amylases; D005978:Glutathione; D003683:Dehydroascorbic Acid