title: Intravitam diagnosis of human rabies by PCR using saliva and cerebrospinal fluid.
abstract: An optimized reverse transcription (RT)-PCR protocol for the intravitam detection of rabies virus genomic RNA was tested with clinical samples obtained from 28 patients suspected of having rabies, 9 of whom were confirmed to have had rabies by postmortem examination. RT-PCR using saliva combined with an immunofluorescence assay performed with skin biopsy samples allowed detection of rabies in the nine patients.
authors.affiliation: Rabies Unit, Institut Pasteur, Paris, France.
authors.forename: P
authors.initials: P
authors.lastname: Crepin
authors.forename: L
authors.initials: L
authors.lastname: Audry
authors.forename: Y
authors.initials: Y
authors.lastname: Rotivel
authors.forename: A
authors.initials: A
authors.lastname: Gacoin
authors.forename: C
authors.initials: C
authors.lastname: Caroff
authors.forename: H
authors.initials: H
authors.lastname: Bourhy
mesh_terms: D002555:Cerebrospinal Fluid / Q000821:virology*; D006801:Humans; D016133:Polymerase Chain Reaction; D012367:RNA, Viral / Q000032:analysis*; D011818:Rabies / Q000175:diagnosis*; D011820:Rabies virus / Q000235:genetics*; D012463:Saliva / Q000821:virology*
chemical_list: D012367:RNA, Viral