Cdc1 is required for growth and Mn2+ regulation in Saccharomyces cerevisiae.
Cdc1 function was initially implicated in bud formation and nuclear division because cdc1(Ts) cells arrested with a small bud, duplicated DNA, and undivided nucleus. Our studies show that Cdc1 is necessary for cell growth at several stages of the cell cycle, as well as in pheromone-treated cells. Thus, Cdc1 depletion might affect bud formation and nuclear division, as well as other cellular processes, by blocking a process involved in general cell growth. Cells depleted of intracellular Mn2+ also exhibit a cdc1-like phenotype and recent results suggested Cdc1 might be a Mn2+-dependent protein. We show that all of the conditional Cdc1(Ts) alleles tested cause cells to become sensitive to Mn2+ depletion. In addition, Cdc1 overproduction alleviates the chelator sensitivity of several Mn2+ homeostasis mutants. These findings are compatible with a model in which Cdc1 regulates intracellular, and in particular cytosolic, Mn2+ levels which, in turn, are necessary for cell growth.
Paidhungat
M
M
Department of Pharmacology and Cancer Biology, Duke University Medical Center, Durham, North Carolina 27710, USA.
Garrett
S
S
D002352:Carrier Proteins / Q000235:genetics; D027682:Cation Transport Proteins; D018797:Cell Cycle Proteins / Q000235:genetics / Q000502:physiology*; D002455:Cell Division; D002473:Cell Wall / Q000502:physiology; D004271:DNA, Fungal / Q000378:metabolism; D004533:Egtazic Acid / Q000494:pharmacology; D006031:Glycosylation; D006056:Golgi Apparatus; D007424:Intracellular Fluid; D008345:Manganese / Q000378:metabolism*; D008565:Membrane Proteins / Q000235:genetics; D016296:Mutagenesis; D012441:Saccharomyces cerevisiae / Q000235:genetics / Q000254:growth & development* / Q000378:metabolism*; D029701:Saccharomyces cerevisiae Proteins
C090459:CDC1 protein, S cerevisiae; D002352:Carrier Proteins; D027682:Cation Transport Proteins; D018797:Cell Cycle Proteins; D004271:DNA, Fungal; D008565:Membrane Proteins; C105116:SMF1 protein, S cerevisiae; D029701:Saccharomyces cerevisiae Proteins; D008345:Manganese; D004533:Egtazic Acid