Sequencing exons 5 to 8 of the p53 gene by MALDI-TOF mass spectrometry.
Matrix-assisted laser desorption ionization time of flight mass spectrometry was used to sequence exons 5 to 8 of the human p53 gene. A single tube procedure was established for target amplification and mass spectrometric (MS) sequencing. The MS sequencing scheme is designed for high throughput and parallel sample processing, and is amenable to full automation. Reliable sequencing data were obtained using fmol sample amounts. The high resolution and accuracy of MS sequencing was demonstrated by direct sequencing of a heterozygous template.
Fu
D J
DJ
Sequenom, Inc., San Diego, CA 92121, USA. dongjing@sequenom-san.com
Tang
K
K
Braun
A
A
Reuter
D
D
Darnhofer-Demar
B
B
Little
D P
DP
O'Donnell
M J
MJ
Cantor
C R
CR
Köster
H
H
D001483:Base Sequence; D004247:DNA; D005091:Exons; D016158:Genes, p53; D006580:Genetic Carrier Screening; D006801:Humans; D009154:Mutation; D016133:Polymerase Chain Reaction; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
D004247:DNA