Separation of the enantiomers of ibuprofen and its major phase I metabolites in urine using capillary electrophoresis.
A capillary electrophoresis method for determination of the enantiomers of ibuprofen and its major phase I metabolites: 2'-hydroxyibuprofen and 2'-carboxyibuprofen in urine samples have been developed. Cyclodextrins and linear dextrins have been investigated as chiral selectors. Simultaneous chiral separation of the enantiomers of ibuprofen, 2'-hydroxyibuprofen and 2'-carboxyibuprofen was obtained using a mixture of dextrin 10 and heptakis (2,3,6-tri-O-methyl)-beta-cyclodextrin in a 2-[N-morpholino]ethanesulphonic acid buffer, pH 5.26. The electroosmotic flow was reversed using hexadimethrine bromide as a buffer additive. The method can be used for the determination of the free enantiomers of ibuprofen, 2'-hydroxyibuprofen and 2'-carboxyibuprofen as well as for the indirect determination of their glucuronic acid conjugates in urine samples.
Bjørnsdottir
I
I
Department of Analytical and Pharmaceutical Chemistry, The Royal Danish School of Pharmacy, Copenhagen.
Kepp
D R
DR
Tjørnelund
J
J
Hansen
S H
SH
D002021:Buffers; D003505:Cyclodextrins; D003912:Dextrins; D019075:Electrophoresis, Capillary / Q000379:methods*; D005965:Glucuronates / Q000302:isolation & purification; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007052:Ibuprofen / Q000031:analogs & derivatives / Q000302:isolation & purification* / Q000378:metabolism / Q000652:urine*; D007202:Indicators and Reagents; D013237:Stereoisomerism; D047392:beta-Cyclodextrins
D002021:Buffers; D003505:Cyclodextrins; D003912:Dextrins; D005965:Glucuronates; D007202:Indicators and Reagents; D047392:beta-Cyclodextrins; C109994:hydroxyibuprofen; C109993:carboxyibuprofen; C000609658:heptakis(2,3,6-tri-O-methyl)-beta-cyclodextrin; D007052:Ibuprofen