gp130RB13-6-positive neural progenitor cells are susceptible to the oncogenic effect of ethylnitrosourea in pre-natal rat brain.
Proliferation-competent rat brain precursor cells of glial lineages are thought to preferentially undergo malignant transformation after transplacental exposure to ethylnitrosourea (EtNU). We recently have reported that monoclonal antibody (mAb) RB13-6 recognizes a developmentally regulated 130 kDa cell surface glycoprotein (gp130RB13-6) transiently expressed by a small subpopulation of glial progenitor cells in pre-natal rat brain. The expression of gp130RB13-6 has now been analysed immunocytochemically in malignant gliomas induced on day 15, 18 or 21 of gestation and in long-term cultures of fetal brain cells (FBC) isolated after in vivo-exposure to EtNU on day 18 of gestation. Malignant gliomas induced at different gestational stages contained varying proportions of gp130RB13-6-positive cells, whereas a subpopulation of proliferative neural progenitor cells exhibiting sustained gp130RB13-6 expression persisted in long-term FBC cultures after 3 months. This subpopulation, which was not selected for in control cultures of FBC derived from buffer-treated rats, gave rise to malignant cell lines after a period of time similar to the latency period required for glioma development in vivo. These data suggest that gp130RB13-6-positive cells of the immature rat nervous system may represent a subset of neural progenitor cells particularly susceptible to the oncogenic effect of EtNU.
Blass-Kampmann
S
S
University of Essen Medical School, Germany.
Bilzer
T
T
Rajewsky
M F
MF
D000818:Animals; D014408:Biomarkers, Tumor / Q000096:biosynthesis; D001932:Brain Neoplasms / Q000737:chemistry / Q000453:epidemiology / Q000473:pathology; D002273:Carcinogens / Q000494:pharmacology*; D002471:Cell Transformation, Neoplastic / Q000187:drug effects* / Q000473:pathology; D005038:Ethylnitrosourea / Q000494:pharmacology*; D005333:Fetus; D006801:Humans; D051907:Lysosomal Membrane Proteins; D008562:Membrane Glycoproteins / Q000032:analysis* / Q000096:biosynthesis; D009380:Neoplasms, Nerve Tissue / Q000737:chemistry / Q000453:epidemiology / Q000473:pathology; D009474:Neurons / Q000187:drug effects* / Q000473:pathology; D051381:Rats; D011919:Rats, Inbred Strains; D013234:Stem Cells / Q000187:drug effects* / Q000473:pathology; D014407:Tumor Cells, Cultured; D052118:Lysosomal-Associated Membrane Protein 1
D014408:Biomarkers, Tumor; D002273:Carcinogens; D005038:Ethylnitrosourea; D051907:Lysosomal Membrane Proteins; D008562:Membrane Glycoproteins; C489116:LAMP1 protein, human; D052118:Lysosomal-Associated Membrane Protein 1; C489117:Lamp1 protein, rat