title: Production of toxic shock syndrome toxin by Staphylococcus aureus isolated from mastitic cow's milk and farm bulk milk.
abstract: The production of toxic shock syndrome toxin (TSST) by Staphylococcus aureus isolated from mastitic cow's milk and farm bulk milk was examined by a reverse passive latex agglutination method (RPLA). TSST was detected in 25 (58.1%) of 43 isolates from clinical mastitic cow's milk, in 79 (76.7%) of 103 isolates from subclinical mastitic cow's milk, and in 95 (75.4%) of 126 isolates from farm bulk milk, respectively. When the quantity of TSST in the isolates was determined by RPLA, the titers ranged from 5 to 2560. TSST with RPLA titers of 640 to 2,560 was produced by 83 (30.5%) of 272 isolates tested. Almost all of the isolates showing RPLA titers of 640 and over produced enterotoxin C, whereas isolates showing titers of 5 to 320 produced enterotoxin C or both enterotoxin A and C. SDS-polyacrylamide gel electrophoresis and isoelectric focusing with immunoblotting showed that the TSST from bovine isolates had same molecular size (22 kDa) and isoelectric point (7.2) as TSST-1 from human isolates. These findings are consistent with previous reports.
authors.affiliation: Department of Bioscience, Faculty of Biotechnology, Fukui Prefectural University, Japan.
authors.forename: S
authors.initials: S
authors.lastname: Takeuchi
authors.forename: K
authors.initials: K
authors.lastname: Ishiguro
authors.forename: M
authors.initials: M
authors.lastname: Ikegami
authors.forename: T
authors.initials: T
authors.lastname: Kaidoh
authors.forename: Y
authors.initials: Y
authors.lastname: Hayakawa
mesh_terms: D000818:Animals; D001427:Bacterial Toxins; D002417:Cattle; D004591:Electrophoresis, Polyacrylamide Gel; D004768:Enterotoxins / Q000096:biosynthesis* / Q000737:chemistry; D005260:Female; D015151:Immunoblotting; D007525:Isoelectric Focusing; D007526:Isoelectric Point; D007841:Latex Fixation Tests / Q000662:veterinary; D008414:Mastitis, Bovine / Q000382:microbiology*; D008970:Molecular Weight; D013203:Staphylococcal Infections / Q000382:microbiology / Q000662:veterinary*; D013211:Staphylococcus aureus / Q000378:metabolism* / Q000472:pathogenicity; D018089:Superantigens
chemical_list: D001427:Bacterial Toxins; D004768:Enterotoxins; D018089:Superantigens; C030107:enterotoxin F, Staphylococcal