[Interaction of short oligonucleotide derivatives with nucleic acids. V. Ligation of short oligonucleotides in tandem on a complimentary DNA template].
A tetranucleotide was highly specifically and quantitatively ligated with a pair of flanking octanucleotides carrying both radioactive and nonradioactive reporter groups. The sequence of the ligation of oligonucleotide components in a tandem on a complementary template was studied. The first stage was found to be the enzyme-catalyzed activation of the phosphate group of octanucleotide, a tandem component that possesses a higher hybridization capacity than the tetramer. It is shown that the introduction of terminal reporter groups into octanucleotides does not decrease the efficiency of their tandem ligation.
Pyshnyĭ
D V
DV
Novosibirsk Institute of Bioorganic Chemistry, Siberian Division, Russian Academy of Sciences, Novosibirsk, Russia.
Krivenko
A A
AA
Lokhov
S G
SG
Ivanova
E M
EM
Dymshits
G M
GM
Zarytova
V F
VF
D001345:Autoradiography; D011088:DNA Ligases / Q000737:chemistry* / Q000235:genetics; D018076:DNA, Complementary / Q000737:chemistry* / Q000235:genetics; D004591:Electrophoresis, Polyacrylamide Gel; D005456:Fluorescent Dyes; D017930:Genes, Reporter / Q000235:genetics; D009691:Nucleic Acid Denaturation; D009693:Nucleic Acid Hybridization; D009841:Oligonucleotides / Q000737:chemistry*; D012091:Repetitive Sequences, Nucleic Acid; D013698:Templates, Genetic
D018076:DNA, Complementary; D005456:Fluorescent Dyes; D009841:Oligonucleotides; D011088:DNA Ligases