Relationship between 2,5-hexanedione concentrations in nerve, serum, and urine alone or under co-treatment with different doses of methyl ethyl ketone, acetone, and toluene.
To ascertain the relationship among 2,5-hexanedione (2,5-HD) concentrations in nerve, serum and urine, rats were injected subcutaneously with 2.6 mmol/kg 2,5-HD alone, or together with 2.6 or 13.0 mmol/kg of methyl ethyl ketone, acetone and toluene. 2,5-HD concentrations in sciatic nerve (NC), serum (SC) and urine (UC) were determined, and the linear regression between each two of NC, SC, and UC were calculated. There was good correlation between NC and SC, SC and UC in the 2,5-HD alone group, and good correlation between NC and SC in the co-treated groups. Co-treatment solvent had little effect on the relationship between SC and NC. 13.0 mmol/kg co-treated solvent tended to decrease the regression coefficients compared with 2.6 mmol/kg co-treated solvent. These results show that SC can be used in estimating NC in the 2,5-HD alone or co-treated groups, and UC can be used in estimating SC in the 2,5-HD alone group.
Zhao
W
W
Department of Public Health and Hygiene, Oita Medical University, Japan. zhaoweny@oitamed.ac.jp
Misumi
J
J
Yasui
T
T
Aoki
K
K
Kimura
T
T
D000096:Acetone / Q000008:administration & dosage / Q000494:pharmacology*; D000704:Analysis of Variance; D000818:Animals; D002074:Butanones / Q000008:administration & dosage / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D004347:Drug Interactions; D006588:Hexanones / Q000097:blood / Q000378:metabolism* / Q000633:toxicity / Q000652:urine; D016018:Least-Squares Analysis; D008297:Male; D009498:Neurotoxins / Q000097:blood / Q000378:metabolism* / Q000633:toxicity / Q000652:urine; D051381:Rats; D017208:Rats, Wistar; D012584:Sciatic Nerve / Q000737:chemistry / Q000378:metabolism*; D012997:Solvents / Q000008:administration & dosage / Q000494:pharmacology*; D014050:Toluene / Q000008:administration & dosage / Q000494:pharmacology*
D002074:Butanones; D006588:Hexanones; D009498:Neurotoxins; D012997:Solvents; D000096:Acetone; D014050:Toluene; C005222:methylethyl ketone; C011269:2,5-hexanedione