Activity staining on polyacrylamide gels of trypsin inhibitors from leaves of sweet potato (Ipomoea batatas L. Lam) varieties.
The failure of activity staining of trypsin inhibitors in crude leaf extracts of sweet potato varieties including Tainong 27, Tainong 34, and Tainong 57 on a 15% sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) gel was prevented by dipping the gels in solutions containing 10-40 mM hydrogen peroxide, 10 mM Tris buffer (pH 7.9) for 30 min before staining.
Hou
W C
WC
Institute of Botany, Academia Sinica, Nankang, Taipei, Taiwan, R.O.C.
Lin
Y H
YH
D000180:Acrylic Resins; D003683:Dehydroascorbic Acid; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods*; D004791:Enzyme Inhibitors / Q000032:analysis*; D005978:Glutathione; D006861:Hydrogen Peroxide; D018515:Plant Leaves / Q000737:chemistry; D010940:Plant Proteins / Q000032:analysis*; D019657:Solanaceae / Q000737:chemistry*; D013194:Staining and Labeling / Q000379:methods*; D014361:Trypsin Inhibitors; D000516:alpha-Amylases / Q000037:antagonists & inhibitors
D000180:Acrylic Resins; D004791:Enzyme Inhibitors; D010940:Plant Proteins; D014361:Trypsin Inhibitors; C016680:polyacrylamide gels; D006861:Hydrogen Peroxide; D000516:alpha-Amylases; D005978:Glutathione; D003683:Dehydroascorbic Acid