Influenza virus-infected epithelial cells present viral antigens to antigen-specific CD8+ cytotoxic T lymphocytes.
We have investigated the mechanisms involved in the clearance of viral infection at the epithelium level by analyzing the activity of influenza virus-specific cytotoxic T lymphocytes (CTL) against virus-infected CMT-93 intestinal epithelial cells. Epithelial cells infected with live influenza virus effectively present viral antigens and were lysed by both homotypic and heterotypic influenza virus-specific CD8+ T cells. These results shed new light on the control of viral infection through the elimination of virus-infected epithelial cells by virus-specific CTL and demonstrate that CMT-93 cells furnish an appropriate model for in vitro evaluation of CTL activity against virus-infected epithelial cells.
Nguyen
H H
HH
Department of Microbiology and The Immunobiology Vaccine Center, University of Alabama at Birmingham, 35294-2170, USA. nghuan@uab.edu
Boyaka
P N
PN
Moldoveanu
Z
Z
Novak
M J
MJ
Kiyono
H
H
McGhee
J R
JR
Mestecky
J
J
D000818:Animals; D000956:Antigens, Viral / Q000276:immunology*; D004847:Epithelial Cells / Q000276:immunology* / Q000821:virology; D006168:Guinea Pigs; D006801:Humans; D009980:Influenza A virus / Q000276:immunology*; D051379:Mice; D008810:Mice, Inbred C57BL; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology*; D014407:Tumor Cells, Cultured
D000956:Antigens, Viral