Interaction of M1 and M2 isozymes pyruvate kinase from human tissues with phospholipids.
The effect of pH and the presence of FBP on the interaction of skeletal muscle (PK-M1) and kidney or tumor meningioma (PK-M2) pyruvate kinase with the phospholipids liposomes were investigated by ultracentrifugation and steady-state kinetics and were compared with those results obtained using the bovine heart (PK-M1) isoenzyme which we previously studied. Pyruvate kinase specific activity increases upon the interaction with liposomes. The activation is specifically sensitive to presence of phosphatidylserine (PS) in liposomes. Liposomes made of phosphatidylcholine + phosphatidylserine mixture are good adsorptive systems for both human and bovine of M-type isozymes at low ionic strength. Interaction of PK-M1 with PS liposomes results in the change of Vmax and K(m) values for PEP without marked effect on Hill coefficients. Addition of PS liposomes to PK-M2 induces hyperbolic saturation curves for PEP.
Dabrowska
A
A
Department of Biochemistry, Wrocław University of Medicine, Poland.
Pietkiewicz
J
J
Dabrowska
K
K
Czapińska
E
E
Danielewicz
R
R
D000327:Adsorption; D000818:Animals; D002417:Cattle; D004912:Erythrocytes / Q000378:metabolism; D005635:Fructosediphosphates / Q000378:metabolism*; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007527:Isoenzymes / Q000378:metabolism*; D007668:Kidney / Q000201:enzymology; D008055:Lipids / Q000097:blood; D008081:Liposomes; D008577:Meningeal Neoplasms / Q000201:enzymology; D008579:Meningioma / Q000201:enzymology; D018482:Muscle, Skeletal / Q000201:enzymology; D010743:Phospholipids / Q000378:metabolism*; D011770:Pyruvate Kinase / Q000378:metabolism*; D014461:Ultracentrifugation
D005635:Fructosediphosphates; D007527:Isoenzymes; D008055:Lipids; D008081:Liposomes; D010743:Phospholipids; D011770:Pyruvate Kinase; C029063:fructose-1,6-diphosphate