Galacto-oligosaccharide production using a recycling cell culture of Sterigmatomyces elviae CBS8119.
Addition of small amounts of Fe2+, Zn2+, Cu2+ and thiamine-HCL to the culture medium was required for promoting the galactooligosaccharide (Gal-OS)-producing activity of Sterigmatomyces elviae CBS8119, when the concentration of yeast extract in the medium was lowered to 0.1 g l-1. Galactooligosaccharide production using a recycling cell culture was performed in a medium containing 360 mg ml-1 of lactose supplemented with optimal concentrations of Fe2+ (1.5 mg l-1 of FeSO4 x 7H2O), Zn3+ (15 mg l-1 of ZnSO4 x 7H2O), Cu2+ (0.5 mg l-1 of CuSO4 x 5H2O) and thiamine-HCL (1 mg l-1). Galacto-oligosaccharide production was maintained at high levels during six cycles of production, with the amount of Gal-OS produced in each cycle being more than 216 mg ml-1 (weight yield of more than 60%.
Onishi
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N
Central Research Laboratories of Ajinomoto Co. Inc., Kawasaki-shi, Japan.
Tanaka
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T
D003470:Culture Media; D005690:Galactose / Q000378:metabolism*; D008670:Metals / Q000494:pharmacology; D003904:Mitosporic Fungi / Q000378:metabolism*; D009844:Oligosaccharides / Q000096:biosynthesis*; D014815:Vitamins / Q000494:pharmacology
D003470:Culture Media; D008670:Metals; D009844:Oligosaccharides; D014815:Vitamins; D005690:Galactose