Hemodynamic monitoring in a model of shock induced by intestinal reperfusion in the rat.
OBJECTIVE
An experimental model to monitor hemodynamics during intestinal reperfusion syndrome is presented.


METHODS
A) Three groups of rats were monitored during four hours: 1) CONTROL: sham operated, 2) Ischemic: clamping the superior mesenteric artery for 60' and 3) Saline: adding 2 ml of saline during the last 15' minutes of ischemia. Blood mean pressure, heart rate, breath rate and diuresis are monitored, while mortality is assessed 48 hours later, B) Blood gases, haematimetry and electrolytes were assessed both in control animals and in rats subjected to intestinal ischemia and reperfusion (at the beginning and concluding the ischemic period, and 5', 60' and 180' following reperfusion).


RESULTS
Mortality rate was 0% for control animals, while 83% for both ischemic-reperfused groups (p < 0.01). Hemodynamic parameters remained stable during intestinal ischemia. Starting reperfusion, blood pressure and diuresis dropped sharply, while heart and breath rates showed a steady increase. The other parameters assessed also remained constant during ischemia. However, during reperfusion acidosis progressed (pH = 7.15, p < 0.01; HCO3 = 8, p < 0.01), hyperkalaemia appeared (6 mEq/l, p < 0.01), and haematocrit rose (56%, p < 0.01).


CONCLUSIONS
This experimental model is easy to establish, allows an appropriate hemodynamic monitoring of shock during intestinal reperfusion syndrome, and seems appropriate for considering the impact of drug intervention.
Ulibarrena
M A
MA
Laboratory of Experimental Surgery, University of The Basque Country, Lejona Vizcaya, Spain.
García-Alonso
I
I
Portugal
V
V
García Redondo
B
B
Méndez
J
J
D000818:Animals; D004195:Disease Models, Animal; D005260:Female; D006439:Hemodynamics / Q000502:physiology*; D007410:Intestinal Diseases / Q000503:physiopathology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D015427:Reperfusion Injury / Q000503:physiopathology*; D012769:Shock / Q000503:physiopathology*; D016019:Survival Analysis