title: Ovalbumin coupled either with murine red blood cells or liposome induces IgG but not IgE antibody production.
abstract: Ovalbumin (OVA) was coupled with murine red blood cells (MRBC) using glutaraldehyde. The OVA-MRBC conjugate induced anti-OVA IgG antibody in mice at almost the same level as OVA in alum. However, no IgE antibody production specific for OVA was observed in OVA-MRBC-injected mice. A significant increase in IGG2a production was obtained with OVA-MRBC immunization, whereas the production of IgG1 predominated in OVA in alum immunization. Am OVA-liposome conjugate induced IgE-specific unresponsiveness in mice in the same manner as OVA-MRBC. Similar results were obtained when antigens other than OVA, such as tetanus toxoid or diphtheria toxoid, were coupled to liposome. These results show the potential of antigen-liposome conjugates for the development of vaccine that induces sufficient IgG antibody production without IgE synthesis.
authors.affiliation: Dept. of Safety Research on Biologics, National Inst. of Infectious Diseases, Tokyo, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Uchida
authors.forename: S
authors.initials: S
authors.lastname: Naito
authors.forename: A
authors.initials: A
authors.lastname: Horino
authors.forename: M
authors.initials: M
authors.lastname: Taneichi
authors.forename: J
authors.initials: J
authors.lastname: Mizuguchi
authors.forename: Y
authors.initials: Y
authors.lastname: Nakano
authors.forename: T
authors.initials: T
authors.lastname: Oka
authors.forename: K
authors.initials: K
authors.lastname: Ookuma
authors.forename: K
authors.initials: K
authors.lastname: Morokuma
authors.forename: S
authors.initials: S
authors.lastname: Sakurai
authors.forename: K
authors.initials: K
authors.lastname: Komuro
mesh_terms: D000534:Alum Compounds; D000818:Animals; D004912:Erythrocytes / Q000276:immunology*; D005260:Female; D007114:Immunization / Q000379:methods; D007073:Immunoglobulin E / Q000096:biosynthesis*; D007074:Immunoglobulin G / Q000096:biosynthesis*; D008081:Liposomes; D051379:Mice; D008807:Mice, Inbred BALB C; D010047:Ovalbumin / Q000276:immunology*; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D000534:Alum Compounds; D007074:Immunoglobulin G; D008081:Liposomes; C041524:aluminum sulfate; D007073:Immunoglobulin E; D010047:Ovalbumin