title: Characterization of CKbeta8 and CKbeta8-1: two alternatively spliced forms of human beta-chemokine, chemoattractants for neutrophils, monocytes, and lymphocytes, and potent agonists at CC chemokine receptor 1.
abstract: Two new members of human beta-chemokine cDNA were isolated based on structural and functional similarities to human leukotactin-1. One of these clones was identical to the previously isolated human beta-chemokine, CKbeta8, whereas the other is a splicing variant of CKbeta8, therefore named CKbeta8-1. CKbeta8 was short in 51 nucleotides (17 amino acids) compared with CKbeta8-1. The mature proteins of CKbeta8-1 and CKbeta8 consisted of 116 and 99 amino acids with calculated molecular weights of 12,500 and 10,950, respectively. Both CKbeta8-1 and CKbeta8 were potent agonists at CCR1. These chemokines chemoattracted neutrophils, monocytes, and lymphocytes. They also significantly suppressed colony formation by human bone marrow, granulocyte-macrophage, erythroid, and multipotential progenitor cells stimulated by combinations of growth factors. To our knowledge, this is the first example that an alternative splicing produces two active beta-chemokines from a single gene.
authors.affiliation: Department of Microbiology and Immunology, Indiana University School of Medicine, Indianapolis, IN 46202, USA.
authors.forename: B S
authors.initials: BS
authors.lastname: Youn
authors.forename: S M
authors.initials: SM
authors.lastname: Zhang
authors.forename: H E
authors.initials: HE
authors.lastname: Broxmeyer
authors.forename: S
authors.initials: S
authors.lastname: Cooper
authors.forename: K
authors.initials: K
authors.lastname: Antol
authors.forename: M
authors.initials: M
authors.lastname: Fraser
authors.forename: B S
authors.initials: BS
authors.lastname: Kwon
mesh_terms: D017398:Alternative Splicing; D001483:Base Sequence; D002118:Calcium / Q000502:physiology; D002478:Cells, Cultured; D019742:Chemokines, CC / Q000235:genetics*; D002634:Chemotaxis, Leukocyte; D002886:Chromosomes, Human, Pair 17; D018076:DNA, Complementary / Q000235:genetics; D005091:Exons; D005796:Genes; D006801:Humans; D007438:Introns; D008214:Lymphocytes / Q000502:physiology; D008969:Molecular Sequence Data; D009000:Monocytes / Q000502:physiology; D009504:Neutrophils / Q000502:physiology; D019707:Receptors, Chemokine / Q000502:physiology; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid
chemical_list: D019742:Chemokines, CC; D018076:DNA, Complementary; D019707:Receptors, Chemokine; D002118:Calcium