title: The effects of Entamoeba histolytica lysates on human colonic mucins.
abstract: Detergent lysates of Entamoeba histolytica trophozoites contained high levels of beta-N acetyl-D-glucosaminidase, beta-N acetyl-D-galactosaminidase and alpha-D-galactosidase activity, and lower but significant levels of five other glycosidases. Although these activities should have been capable of largely degrading the oligosaccharide side-chains of human colonic mucin, in fact only about one third of high MW mucin was degraded in 72 h and trypsin alone produced a similar effect. There was no evidence that these glycosidases were excreted and we conclude that they are unlikely to represent significant virulence factors for E. histolytica.
authors.affiliation: Department of Medical Parasitology, London School of Hygiene and Tropical Medicine, UK.
authors.forename: W M
authors.initials: WM
authors.lastname: Spice
authors.forename: J P
authors.initials: JP
authors.lastname: Ackers
mesh_terms: D000818:Animals; D002847:Chromatography, Agarose; D003106:Colon / Q000737:chemistry* / Q000469:parasitology; D004748:Entamoeba histolytica / Q000737:chemistry*; D004749:Entamoebiasis / Q000469:parasitology; D006026:Glycoside Hydrolases / Q000737:chemistry; D006790:Host-Parasite Interactions; D006801:Humans; D009077:Mucins / Q000737:chemistry*; D009596:Nitrophenols / Q000737:chemistry; D013552:Swine
chemical_list: D009077:Mucins; D009596:Nitrophenols; D006026:Glycoside Hydrolases; C024836:4-nitrophenol