Bovine herpesvirus 1-induced apoptosis: phenotypic characterization of susceptible peripheral blood mononuclear cells.
Bovine herpesvirus 1 (BHV-1), a member of the Alphaherpesvirinae, induces apoptotic cell death in peripheral blood mononuclear cells (PBMC). To investigate the process by which BHV-1 induces apoptosis, we determined the susceptibility of the three main PBMC subpopulations to BHV-1-induced apoptosis. This study shows that BHV-1 can induce apoptosis individually in T lymphocytes, B lymphocytes and monocytes. This conclusion is based on the following findings: (i) BHV-1 substantially reduces the percentages of viable T and B lymphocytes in PBMCs. (ii) Concomitant detection of cell phenotype and apoptosis indeed showed higher percentages of apoptotic T lymphocytes and B lymphocytes in BHV-1-infected PBMCs than in mock-infected cells. (iii) Each individual PBMC subpopulations (B lymphocytes, T lymphocytes and monocytes) undergo apoptosis when incubated with BHV-1. These data also suggest that BHV-1 does not require the recruitment of one or more individual PBMC subpopulations (e.g. cytotoxic cells) to induce apoptosis. Finally, we observed that BL-3 cells which have been characterized as bovine tumoral B lymphocytes also undergo apoptosis when incubated with BHV-1. Therefore, the use of the BL-3 cell line provides a new experimental model to investigate the apoptotic process induced by BHV-1 in vitro.
Hanon
E
E
Department of Immunology/Vaccinology, Faculty of Veterinary Medicine, University of Liège, Belgium.
Lambot
M
M
Hoornaert
S
S
Lyaku
J
J
Pastoret
P P
PP
D000818:Animals; D017209:Apoptosis; D001402:B-Lymphocytes / Q000821:virology; D002417:Cattle; D002460:Cell Line; D002470:Cell Survival; D007242:Herpesvirus 1, Bovine / Q000472:pathogenicity*; D007963:Leukocytes, Mononuclear / Q000821:virology*; D009000:Monocytes / Q000821:virology; D010641:Phenotype; D013601:T-Lymphocytes / Q000821:virology; D014407:Tumor Cells, Cultured