title: Characterization of extracellular menadion-catalyzed H2O2 production by NIH/3T3 cells.
abstract: The extracellular menadione-catalyzed H2O2 production by NIH/3T3 cells was expected to depend on plasma membrane-bound NAD(P)H:quinone oxidoreductase. This enzyme was estimated to be a flavoprotein with the molecular mass of 70 KDa. Km values of plasma membrane-bound NAD(P)H:quinone oxidoreductase producing H2O2 were 60 microM for NADH and 150 microM for NADPH. Ca2+ ionophore A23187 controlled menadione-catalyzed H2O2 production by the cells in time- and concentration-dependent manner.
authors.affiliation: Kobe Gakuin Women's College, Japan.
authors.forename: S
authors.initials: S
authors.lastname: Yamashoji
mesh_terms: D016475:3T3 Cells / Q000187:drug effects / Q000378:metabolism*; D000818:Animals; D000001:Calcimycin / Q000494:pharmacology; D002462:Cell Membrane / Q000187:drug effects / Q000378:metabolism; D001728:Dicumarol / Q000494:pharmacology; D004791:Enzyme Inhibitors / Q000494:pharmacology; D006861:Hydrogen Peroxide / Q000378:metabolism*; D007476:Ionophores / Q000494:pharmacology; D051379:Mice; D016660:NAD(P)H Dehydrogenase (Quinone) / Q000187:drug effects / Q000378:metabolism; D009249:NADP / Q000378:metabolism; D014812:Vitamin K / Q000494:pharmacology*
chemical_list: D004791:Enzyme Inhibitors; D007476:Ionophores; D014812:Vitamin K; D000001:Calcimycin; D009249:NADP; D001728:Dicumarol; D006861:Hydrogen Peroxide; D016660:NAD(P)H Dehydrogenase (Quinone)