title: Membrane-permeant esters of phosphatidylinositol 3,4,5-trisphosphate.
abstract: Phosphoinositide 3-OH kinases and their products, D-3 phosphorylated phosphoinositides, are increasingly recognized as crucial elements in many signaling cascades. A reliable means to introduce these lipids into intact cells would be of great value for showing the physiological roles of this pathway and for testing the specificity of pharmacological inhibitors of the kinases. We have stereospecifically synthesized di-C8-PIP3/AM and di-C12-PIP3/AM, the heptakis(acetoxymethyl) esters of dioctanoyl- and dilauroylphosphatidylinositol 3,4,5-trisphosphate, in 14 steps from myo-inositol. The ability of these uncharged lipophilic derivatives to deliver phosphatidylinositol 3,4,5-trisphosphate across cell membranes was demonstrated on 3T3-L1 adipocytes and T84 colon carcinoma monolayers. Insulin stimulation of hexose uptake into adipocytes was inhibited by the kinase inhibitor wortmannin and was largely restored by di-C8-PIP3/AM, which had no effect in the absence of insulin. Thus phosphatidylinositol 3,4,5-trisphosphate or a metabolite was necessary but not sufficient for stimulation of hexose transport. In T84 epithelial monolayers, di-C12-PIP3/AM mimicked epidermal growth factor in inhibiting chloride secretion and potassium efflux, suggesting that phosphatidylinositol 3,4, 5-trisphosphate was sufficient to modulate these fluxes and mediate epidermal growth factor's action.
authors.affiliation: Department of Pharmacology and Howard Hughes Medical Institute, University of California San Diego, La Jolla, California 92093-0647, USA.
authors.forename: T
authors.initials: T
authors.lastname: Jiang
authors.forename: G
authors.initials: G
authors.lastname: Sweeney
authors.forename: M T
authors.initials: MT
authors.lastname: Rudolf
authors.forename: A
authors.initials: A
authors.lastname: Klip
authors.forename: A
authors.initials: A
authors.lastname: Traynor-Kaplan
authors.forename: R Y
authors.initials: RY
authors.lastname: Tsien
mesh_terms: D016475:3T3 Cells; D017667:Adipocytes / Q000187:drug effects / Q000378:metabolism; D000730:Androstadienes / Q000494:pharmacology; D000818:Animals; D002463:Cell Membrane Permeability; D004815:Epidermal Growth Factor / Q000378:metabolism; D004952:Esters; D006601:Hexoses / Q000378:metabolism; D007328:Insulin / Q000494:pharmacology; D009682:Magnetic Resonance Spectroscopy; D051379:Mice; D018129:Phosphatidylinositol Phosphates / Q000737:chemistry / Q000378:metabolism* / Q000494:pharmacology; D014407:Tumor Cells, Cultured; D000077191:Wortmannin
chemical_list: D000730:Androstadienes; D004952:Esters; D006601:Hexoses; D007328:Insulin; D018129:Phosphatidylinositol Phosphates; C060974:phosphatidylinositol 3,4,5-triphosphate; D004815:Epidermal Growth Factor; D000077191:Wortmannin