Elimination of alkaline phosphatases from circulation by the galactose receptor. Different isoforms are cleared at various rates.
Three isoforms of human alkaline phosphatase (liver, bone and placental ALP) were purified and their elimination studied after intravenous injection in rats. The rates of elimination were significantly inhibited by prior injection of asialofetuin, indicating that the uptake was mediated by the galactose receptor in liver. Their relative clearance rates differed, being rapid for the bone ALP, significantly slower for the liver isoform and very slow for the placental ALP. The bone ALP showed a rapid initial clearance, apparently related to its large glycan heterogeneity and to the presence of molecules with a low sialic acid content. When isolated from serum the liver and bone ALP isoforms showed clearance rates differing slightly from those of the organ derived forms. We conclude that differences in carbohydrate structure and amount of sialic acid of the three isoforms result in various clearance rates. These differences will also affect their serum concentrations as well as the composition and heterogeneity of the individual isoforms in serum.
Blom
E
E
Tromsø College, Faculty of Health Sciences, Norway.
Ali
M M
MM
Mortensen
B
B
Huseby
N E
NE
D000293:Adolescent; D000328:Adult; D000469:Alkaline Phosphatase / Q000097:blood*; D000818:Animals; D001842:Bone and Bones / Q000201:enzymology; D002648:Child; D002675:Child, Preschool; D005260:Female; D006801:Humans; D007527:Isoenzymes / Q000097:blood*; D007700:Kinetics; D008099:Liver / Q000201:enzymology; D008297:Male; D008657:Metabolic Clearance Rate; D010920:Placenta / Q000201:enzymology; D051381:Rats; D017207:Rats, Sprague-Dawley; D011956:Receptors, Cell Surface / Q000097:blood*
D007527:Isoenzymes; D011956:Receptors, Cell Surface; C039707:galactose receptor; D000469:Alkaline Phosphatase