title: Biological parameters and molecular markers of clone CL Brener--the reference organism of the Trypanosoma cruzi genome project.
abstract: Clone CL Brener is the reference organism used in the Trypanosoma cruzi Genome Project. Some biological parameters of CL Brener were determined: (a) the doubling time of epimastigote forms cultured in liver infusion-tryptose (LIT) medium at 28 degrees C is 58 +/- 13 hr; (b) differentiation of epimastigotes to metacyclic trypomastigotes is obtained by incubation in LIT-20% Grace's medium; (c) trypomastigotes infect mammalian cultured cells and perform the complete intracellular cycle at 33 and 37 degrees C; (d) blood forms are highly infective to mice; (e) blood forms are susceptible to nifurtimox and benznidazole. The molecular typing of CL Brener has been determined: (a) isoenzymatic profiles are characteristic of zymodeme ZB; (b) PCR amplification of a 24S alpha ribosomal RNA sequence indicates it belongs to T. cruzi lineage 1; (c) schizodeme, randomly amplified polymorphic DNA (RAPD) and DNA fingerprinting analyses were performed.
authors.affiliation: Instituto de Química, Universidade de São Paulo, Brasil. bszodnas@quim.iq.usp.br
authors.forename: B
authors.initials: B
authors.lastname: Zingales
authors.forename: M E
authors.initials: ME
authors.lastname: Pereira
authors.forename: K A
authors.initials: KA
authors.lastname: Almeida
authors.forename: E S
authors.initials: ES
authors.lastname: Umezawa
authors.forename: N S
authors.initials: NS
authors.lastname: Nehme
authors.forename: R P
authors.initials: RP
authors.lastname: Oliveira
authors.forename: A
authors.initials: A
authors.lastname: Macedo
authors.forename: R P
authors.initials: RP
authors.lastname: Souto
mesh_terms: D000818:Animals; D001483:Base Sequence; D015415:Biomarkers / Q000032:analysis*; D019976:Cloning, Organism; D018503:Genome, Protozoan; D008969:Molecular Sequence Data; D014349:Trypanosoma cruzi / Q000235:genetics*
chemical_list: D015415:Biomarkers