title: Diagnosis of dengue by using reverse transcriptase-polymerase chain reaction.
abstract: A rapid identification of dengue viruses from clinical samples by using a nested reverse transcriptase-polymerase chain reaction (RT-PCR) procedure was carried out for diagnostic and epidemiological purposes. RT-PCR identified DEN-1 and DEN-2 viruses in 41% (41/100) of previously confirmed cases and provided an accurate confirmation of DHF in four fatal cases. RT-PCR was also useful for detecting and typing dengue viruses in suspected cases, allowing a rapid identification of new serotypes in endemic areas.
authors.affiliation: Departamento de Virologia, Instituto Oswaldo Cruz, RJ, Brasil. marizepm@gene.dbbm.fiocruz.br
authors.forename: M P
authors.initials: MP
authors.lastname: Miagostovich
authors.forename: F B
authors.initials: FB
authors.lastname: dos Santos
authors.forename: E S
authors.initials: ES
authors.lastname: de Araújo
authors.forename: J
authors.initials: J
authors.lastname: Dias
authors.forename: H G
authors.initials: HG
authors.lastname: Schatzmayr
authors.forename: R M
authors.initials: RM
authors.lastname: Nogueira
mesh_terms: D001938:Brazil / Q000453:epidemiology; D003715:Dengue / Q000175:diagnosis* / Q000453:epidemiology; D003716:Dengue Virus / Q000302:isolation & purification*; D006801:Humans; D016133:Polymerase Chain Reaction / Q000379:methods*