High temperature conjugation of proteins with carbohydrates.
A new procedure was used to conjugate lactose and dextran with BSA without using coupling or activating reagents. The method is simple, rapid and cheap. Reducing sugars covalently bind to proteins when lyophilized together and briefly heated to a high temperature.
Boratyński
J
J
Department of Tumor Immunology, Ludwik Hirszfeld Institute of Immunology and Experimental Therapy, Polish Academy of Sciences, Wrocław. borat@immuno.iitd.pan.wroc.pl
Roy
R
R
D000818:Animals; D003429:Cross Reactions; D003911:Dextrans / Q000138:chemical synthesis / Q000737:chemistry; D006023:Glycoproteins / Q000138:chemical synthesis* / Q000276:immunology*; D007074:Immunoglobulin G / Q000276:immunology; D007785:Lactose / Q000737:chemistry; D008070:Lipopolysaccharides / Q000737:chemistry; D011817:Rabbits; D012710:Serum Albumin, Bovine / Q000138:chemical synthesis / Q000737:chemistry; D013696:Temperature; D014357:Trypsin / Q000737:chemistry
D003911:Dextrans; D006023:Glycoproteins; D007074:Immunoglobulin G; D008070:Lipopolysaccharides; C043578:bovine serum albumin-dextran conjugate; D012710:Serum Albumin, Bovine; D014357:Trypsin; D007785:Lactose