title: Phosphopeptides derived from in vitro phosphorylated E. coli RNA polymerase bind to DNA and affect DNA transcription.
abstract: E. Coli RNA polymerase was phosphorylated with protein kinase CKII and allowed to bind to pBR322. After digestion of the RNA polymerase-pBR322 complex with proteinase K, the phosphopeptides that remained bound to DNA were extracted and analyzed. These phosphopeptides are able to bind again to DNA and to inhibit transcription.
authors.affiliation: Dipartimento di Biologia Cellulare e Molecolare, Sezione Fisiologia e Biofisica, Università di Perugia, Italy.
authors.forename: E
authors.initials: E
authors.lastname: Cardellini
authors.forename: G
authors.initials: G
authors.lastname: Piccinini
authors.forename: G L
authors.initials: GL
authors.lastname: Gianfranceschi
mesh_terms: D047390:Casein Kinase II; D004247:DNA / Q000378:metabolism*; D012321:DNA-Directed RNA Polymerases / Q000378:metabolism*; D015180:Electrophoresis, Gel, Two-Dimensional; D019286:Endopeptidase K / Q000378:metabolism; D004926:Escherichia coli / Q000201:enzymology; D010748:Phosphopeptides / Q000378:metabolism*; D010766:Phosphorylation; D017346:Protein Serine-Threonine Kinases / Q000378:metabolism; D014158:Transcription, Genetic
chemical_list: D010748:Phosphopeptides; D004247:DNA; D047390:Casein Kinase II; D017346:Protein Serine-Threonine Kinases; D012321:DNA-Directed RNA Polymerases; D019286:Endopeptidase K