title: The Rhizobium meliloti ExoK and ExsH glycanases specifically depolymerize nascent succinoglycan chains.
abstract: The Rhizobium meliloti ExoK and ExsH glycanases have been proposed to contribute to production of low molecular weight (LMW) succinoglycan by depolymerizing high molecular weight succinoglycan chains in R. meliloti cultures. We expressed and purified ExoK and ExsH and determined that neither enzyme can extensively cleave succinoglycan prepared from R. meliloti cultures, although neutral/heat treatment and acid/heat treatment convert succinoglycan to forms that can be cleaved efficiently by both enzymes. These results were somewhat surprising, given that the exoK+ and exsH+ genes play a crucial role in production of LMW succinoglycan in R. meliloti cultures. We demonstrated by Western blot analyses that R. meliloti expresses ExoK and ExsH, that both proteins can be detected extracellularly, and that ExsH secretion depends on the prsD+/prsE+ genes, consistent with previous predictions based on mutant analyses. Furthermore, we determined that the depolymerization activities associated with purified ExoK and ExsH are comparable with exoK+ and exsH+-dependent depolymerization activities expressed in R. meliloti cultures. We resolved the apparent contradiction between the results of our previous genetic analyses and depolymerization assays by determining that ExoK and ExsH can cleave high molecular weight succinoglycan that is being produced actively by R. meliloti, but not succinoglycan that has accumulated in cultures, to yield LMW succinoglycan. We propose that ExoK and ExsH dynamically regulate the molecular weight distribution of succinoglycan by cleaving nascent succinoglycan only during a limited period after its synthesis, perhaps before it undergoes a time-dependent change in its conformation or aggregation state.
authors.affiliation: Department of Biology, Massachusetts Institute of Technology, Cambridge, MA 02139, USA.
authors.forename: G M
authors.initials: GM
authors.lastname: York
authors.forename: G C
authors.initials: GC
authors.lastname: Walker
mesh_terms: D001426:Bacterial Proteins; D005798:Genes, Bacterial; D006026:Glycoside Hydrolases / Q000378:metabolism*; D008970:Molecular Weight; D011135:Polysaccharides, Bacterial / Q000737:chemistry / Q000378:metabolism*; D016962:Sinorhizobium meliloti / Q000201:enzymology*; D012995:Solubility; D013379:Substrate Specificity
chemical_list: D001426:Bacterial Proteins; D011135:Polysaccharides, Bacterial; C056779:succinoglycan; C112237:ExoK glycanase, Rhizobium meliloti; D006026:Glycoside Hydrolases; C020668:glycanase