Chromatographic analysis of polysaccharides extracted from Chinese Indocalamus tesselatus.
Three fractions of polysaccharides that have high biological activity were extracted from Chinese bamboo leaves (Indocalamus Tesselatus) with 0.9% sodium chloride, 1% ammonium oxalate and 85% ethanol. Their component sugars were investigated by paper chromatography developed with n-butanol-pyridine-water (6:4:3 by vol), and by gas chromatography with crown ester stationary-phase-fused silica capillary column. Their monosaccharide compositions are different from each other with glucuronic acid, galactose, arabinose, mannose, glucose, and xylose (52, 18, 9, 7 and 6%, respectively) in one fraction, only glucuronic acid in another and glucuronic acid, fucose, arabinose and xylose (36, 31, 24 and 9%, respectively) in the third fraction. The protection effect of two polysaccharides against experimental hepatitis was studied in mice.
Ding
Y Q
YQ
Experimental Centre of Nanyang Institute of Science and Technology, China.
Li
Y
Y
Chen
C Y
CY
Elmahadi
E A
EA
Xu
H B
HB
D000818:Animals; D002849:Chromatography, Gas; D002854:Chromatography, Paper; D002855:Chromatography, Thin Layer; D051379:Mice; D018515:Plant Leaves / Q000737:chemistry*; D011134:Polysaccharides / Q000032:analysis* / Q000302:isolation & purification
D011134:Polysaccharides