Transverse formamide gradients as a simple and easy way to optimise DNA single-strand conformation polymorphism analysis.
Although widely used, the detection of DNA mutations by the single-strand conformation polymorphism (SSCP) method is often hampered by the need to examine a large set of electrophoretic conditions in order to select the one suited to the DNA sequence under study. We show here that the use of transverse chemical gradient gels allows for a quick and easy optimisation of SSCP analysis, as exemplified on two mutations in exon 2 of the alpha-1-antitrypsin gene.
Paccoud
B
B
INSERM Unité 295, Faculté de Médecine-Pharmacie de Rouen, Avenue de l'Université, F-76800 Saint-Etienne du Rouvray, France.
Bourguignon
J
J
Diarra-Mehrpour
M
M
Martin
J P
JP
Sesboüé
R
R
D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods*; D005091:Exons; D005559:Formamides; D009154:Mutation; D018807:Polymorphism, Single-Stranded Conformational; D000515:alpha 1-Antitrypsin / Q000235:genetics
D005559:Formamides; D000515:alpha 1-Antitrypsin; C031066:formamide