title: Crystal structure of the type-2 Cu depleted laccase from Coprinus cinereus at 2.2 A resolution.
abstract: Laccase catalyses the oxidation of a variety of organic substrates coupled to the reduction of oxygen to water. It is widely believed to be the simplest representative of the ubiquitous blue multi-copper oxidase family. Laccase is implicated in a wide spectrum of biological activities and, in particular, plays a key role in morphogenesis, development and lignin metabolism in fungi and plants. The structure of laccase from the fungus Coprinus cinereus has been determined by X-ray crystallography at a resolution of 2.2 A. Laccase is a monomer composed of three cupredoxin-like beta-sandwich domains, similar to that found in ascorbate oxidase. In contrast to ascorbate oxidase, however, the mononuclear type-1 Cu site lacks the axial methionine ligand and so exhibits trigonal planar coordination, consistent with its elevated redox potential. Crucially, the structure is trapped in a Cu depleted form in which the putative type-2 Cu atom is completely absent, but in which the remaining type-1 and type-3 Cu sites display full occupancy. Type-2 Cu depletion has unexpected consequences for the coordination of the remaining type-3 Cu atoms.
authors.affiliation: Department of Chemistry, University of York, Heslington, UK.
authors.forename: V
authors.initials: V
authors.lastname: Ducros
authors.forename: A M
authors.initials: AM
authors.lastname: Brzozowski
authors.forename: K S
authors.initials: KS
authors.lastname: Wilson
authors.forename: S H
authors.initials: SH
authors.lastname: Brown
authors.forename: P
authors.initials: P
authors.lastname: Ostergaard
authors.forename: P
authors.initials: P
authors.lastname: Schneider
authors.forename: D S
authors.initials: DS
authors.lastname: Yaver
authors.forename: A H
authors.initials: AH
authors.lastname: Pedersen
authors.forename: G J
authors.initials: GJ
authors.lastname: Davies
mesh_terms: D000595:Amino Acid Sequence; D001204:Ascorbate Oxidase / Q000737:chemistry; D001400:Azurin / Q000031:analogs & derivatives / Q000737:chemistry; D001665:Binding Sites; D003300:Copper; D003302:Coprinus / Q000201:enzymology*; D018360:Crystallography, X-Ray / Q000379:methods; D006801:Humans; D042845:Laccase; D008969:Molecular Sequence Data; D010088:Oxidoreductases / Q000737:chemistry*; D011487:Protein Conformation; D017433:Protein Structure, Secondary; D011994:Recombinant Proteins / Q000737:chemistry
chemical_list: D011994:Recombinant Proteins; C050107:cupredoxin; D001400:Azurin; D003300:Copper; D010088:Oxidoreductases; D042845:Laccase; D001204:Ascorbate Oxidase