title: Compact growth of Staphylococcus haemolyticus in soft agar is not due to hydrophobic interaction between the cocci.
abstract: It was hypothesized that the formation of compact colony in soft-agar both in the presence and absence of serum, characteristic mainly for strains of the species Staphylococcus haemolyticus among coagulase-negative staphylococci [Szúcs et al. Acta Microbiologica Hungarica 40:181-189 (1993)] was due to hydrophobic interaction between cocci. The effect of a number of surface active agents on this phenomenon was examined. Neither 0.1% and 1%. Tween-80 nor 5% and 10% ethylene glycol and polyethylene glycol nor 0.1%-4% trypsin influenced the colony morphology in soft-agar prepared in modified Staphylococcus 110 broth. Bovine lactoferrin and apolactoferrin at concentrations of 0.1%-0.4% made compact colonies transient to diffuse ones. Thus, cocci are not adhered to each other in compact ball-like colonies by hydrophobic interaction or trypsin-sensitive proteins. It is possible that still unknown polysaccharide-binding proteins or other trypsin-resistant proteins are responsible for the formation of compact colonies by Staphylococcus haemolyticus in soft-agar.
authors.affiliation: Department of Radiology, University Medical School of Debrecen, Hungary.
authors.forename: Z I
authors.initials: ZI
authors.lastname: Godó
authors.forename: E
authors.initials: E
authors.lastname: Magyar
authors.forename: I
authors.initials: I
authors.lastname: Andirkó
authors.forename: F
authors.initials: F
authors.lastname: Rozgonyi
mesh_terms: D001422:Bacterial Adhesion / Q000187:drug effects*; D003470:Culture Media; D003902:Detergents / Q000494:pharmacology; D019855:Ethylene Glycol / Q000494:pharmacology; D005026:Ethylene Glycols / Q000494:pharmacology; D006801:Humans; D007782:Lactoglobulins / Q000494:pharmacology; D011092:Polyethylene Glycols / Q000494:pharmacology; D011136:Polysorbates / Q000494:pharmacology; D013210:Staphylococcus / Q000187:drug effects* / Q000201:enzymology / Q000254:growth & development / Q000502:physiology; D014357:Trypsin / Q000494:pharmacology
chemical_list: D003470:Culture Media; D003902:Detergents; D005026:Ethylene Glycols; D007782:Lactoglobulins; D011136:Polysorbates; D011092:Polyethylene Glycols; D014357:Trypsin; D019855:Ethylene Glycol