title: Regulation of interleukin-13 receptor constituents on mature human B lymphocytes.
abstract: Human B cells stimulated through both their immunoglobulin and CD40 receptors up-regulate 745 +/- 51 interleukin (IL)-13 ligand binding sites with an affinity of 0.91 +/- 0.08 nM within 24 h. IL-13 binds primarily to the IL-13Ralpha1 with subsequent sequestration of the IL-4Ralpha into the complex. IL-13Ralpha1 may also be found in those receptors capable of binding IL-4. gamma chain (gammac) participates in receptors capable of binding IL-4 but is not found in association with bound IL-13. Dimeric receptors composed of the IL-4Ralpha complexed with either the IL-13Ralpha1 or gammac occur simultaneously within defined B cell populations. mRNAs for all receptor constituents are increased subsequent to immunoglobulin stimulation alone, while maximal expression of IL-13Ralpha1 is more dependent upon co-stimulation of immunoglobulin and CD40 receptors. mRNA levels for IL-13Ralpha1 vary over a wider range subsequent to surface stimulation than other receptor components. Although gammac is not bound to IL-13 in B cells under the conditions evaluated, it may influence IL-13 binding by competing with IL-13Ralpha1 for association/sequestration with the IL-4Ralpha chain. IL-13Ralpha2 does not participate in the IL-13 receptor that is up-regulated upon activation of quiescent tonsillar B lymphocytes, although mRNA for the protein may be found in the centroblastic fraction of tonsillar cells.
authors.affiliation: Roger Williams Medical Center/Brown University, Providence, Rhode Island 02908, USA.
authors.forename: H
authors.initials: H
authors.lastname: Ogata
authors.forename: D
authors.initials: D
authors.lastname: Ford
authors.forename: N
authors.initials: N
authors.lastname: Kouttab
authors.forename: T C
authors.initials: TC
authors.lastname: King
authors.forename: N
authors.initials: N
authors.lastname: Vita
authors.forename: A
authors.initials: A
authors.lastname: Minty
authors.forename: J
authors.initials: J
authors.lastname: Stoeckler
authors.forename: D
authors.initials: D
authors.lastname: Morgan
authors.forename: C
authors.initials: C
authors.lastname: Girasole
authors.forename: J W
authors.initials: JW
authors.lastname: Morgan
authors.forename: A L
authors.initials: AL
authors.lastname: Maizel
mesh_terms: D001402:B-Lymphocytes / Q000166:cytology / Q000187:drug effects / Q000276:immunology*; D002455:Cell Division; D002478:Cells, Cultured; D016207:Cytokines / Q000494:pharmacology*; D017931:DNA Primers; D019281:Dimerization; D006801:Humans; D018793:Interleukin-13 / Q000378:metabolism; D053661:Interleukin-13 Receptor alpha1 Subunit; D015847:Interleukin-4 / Q000378:metabolism; D007378:Interleukins / Q000494:pharmacology*; D008213:Lymphocyte Activation; D046911:Macromolecular Substances; D014066:Palatine Tonsil; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000096:biosynthesis; D018123:Receptors, Interleukin / Q000096:biosynthesis*; D053660:Receptors, Interleukin-13; D019948:Receptors, Interleukin-4 / Q000096:biosynthesis*; D011994:Recombinant Proteins / Q000494:pharmacology; D014158:Transcription, Genetic / Q000187:drug effects / Q000276:immunology; D014409:Tumor Necrosis Factor-alpha / Q000494:pharmacology; D015854:Up-Regulation / Q000187:drug effects
chemical_list: D016207:Cytokines; D017931:DNA Primers; C508181:IL13RA1 protein, human; D018793:Interleukin-13; D053661:Interleukin-13 Receptor alpha1 Subunit; D007378:Interleukins; D046911:Macromolecular Substances; D012333:RNA, Messenger; D018123:Receptors, Interleukin; D053660:Receptors, Interleukin-13; D019948:Receptors, Interleukin-4; D011994:Recombinant Proteins; D014409:Tumor Necrosis Factor-alpha; D015847:Interleukin-4