[Methodology of obtaining and digital processing of adhesive cells].
The paper deals with the methodological features of investigations of the interaction of cells and foreign material surfaces by scanning electron microscopy. The proposed technique for obtaining electron microscopic images results in their easy binarization and further treatment using computer software. The specificity of obtaining electron microphotographs permits detailed quantitative image analysis. This approach enables one to study both quantity of objects and their individual morphologies, as well as to define distributions of objects by their diameter, area, etc. Human platelet adhesion to glass and metal is considered as an example.
Vasin
S L
SL
Titushkin
I A
IA
Prokopenko
R A
RA
Rozanova
I B
IB
Sevast'ianov
V I
VI
D000535:Aluminum; D002448:Cell Adhesion; D005898:Glass; D006801:Humans; D007091:Image Processing, Computer-Assisted; D008855:Microscopy, Electron, Scanning; D010784:Photomicrography; D010973:Platelet Adhesiveness; D012984:Software; D013997:Time Factors
D000535:Aluminum