Morphogenic activity of fibroblast growth factor-2 on primary neural precursor cells in three-dimensional culture.
Mouse neural precursor cells (NPC) were dissociated from fetal heads at the 10th day of gestation. When clumps of NPC were cultured in collagen gel, they grew and reorganized neural tube-like structures in medium containing fetal calf serum at 10% and supplemented with insulin, transferrin, cholera toxin and selenite. However, dissociated NPC died when they were cultured in collagen gel at low density in the same medium. Addition of fibroblast growth factor-2 (FGF-2) to this culture stimulated growth of NPC and formation of neural tube-like structures. The requirement for FGF-2 disappeared in high seeding density culture: they grew and formed neural tube-like structures without FGF-2. The structures formed in collagen gel were immunohistochemically positive against anti-FGF-2 antibody. The results show that the three-dimensional culture system provides a useful tool to study the roles of FGF-2 in morphogenesis of the central nervous system.
Hiratochi
M
M
Graduate School of Integrated Science, Yokohama City University, Yokohama.
Fujiwara
A
A
Kitani
H
H
Iguchi
T
T
Sakakura
T
T
Tomooka
Y
Y
D000818:Animals; D002452:Cell Count; D018929:Cell Culture Techniques / Q000379:methods*; D002455:Cell Division / Q000187:drug effects; D002478:Cells, Cultured; D002490:Central Nervous System / Q000737:chemistry / Q000196:embryology*; D003094:Collagen; D017077:Culture Media, Conditioned / Q000737:chemistry; D016222:Fibroblast Growth Factor 2 / Q000032:analysis / Q000494:pharmacology*; D005782:Gels; D006133:Growth Substances / Q000494:pharmacology; D051379:Mice; D009024:Morphogenesis; D009474:Neurons / Q000737:chemistry / Q000166:cytology*
D017077:Culture Media, Conditioned; D005782:Gels; D006133:Growth Substances; D016222:Fibroblast Growth Factor 2; D003094:Collagen