Blastomeres and cells with mesendodermal fates of carp embryos express cth1, a member of the TIS11 family of primary response genes.
The carp cth1 gene, related to the mammalian TIS11 family of primary response genes, encodes a novel fish protein with two putative CCCH zinc fingers. This report describes the RNA expression of this gene during cleavage, blastula and gastrula stages of carp embryos. Cth1 mRNA is present in all cleavage stage blastomeres as a maternal message. After the late blastula stage, the maternal expression decreases, revealing a spot of higher expression at the margin of the blastoderm of the dome stage embryo. Further decrease of the maternal message reveals a ring of cth1 expressing cells at the blastoderm margin from the stage of 40% epiboly onwards. By alpha-amanitin treatment we established that this local cth1 expression is of zygotic origin. At the onset of gastrulation the cells of the cth1 ring involute, starting with those in the shield region, and at approximately 60% epiboly the ring is fully involuted and occupies the hypoblast layer. All cth1 transcripts have disappeared at completion of epiboly. We discuss a possible role for the putative cth1 protein during cleavage and gastrulation.
Stevens
C J
CJ
Experimental Zoology Group, Wageningen Institute of Animal Sciences, Wageningen Agricultural University, The Netherlands.
Schipper
H
H
Samallo
J
J
Stroband
H W
HW
te Kronnie
T
T
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D001757:Blastomeres / Q000378:metabolism*; D002347:Carps / Q000196:embryology* / Q000235:genetics; D004268:DNA-Binding Proteins / Q000096:biosynthesis*; D004625:Embryo, Nonmammalian / Q000378:metabolism; D004707:Endoderm / Q000378:metabolism*; D005260:Female; D017874:Immediate-Early Proteins; D008297:Male; D008648:Mesoderm / Q000378:metabolism*; D008969:Molecular Sequence Data; D005810:Multigene Family; D014176:Protein Biosynthesis; D011506:Proteins / Q000235:genetics; D014157:Transcription Factors / Q000096:biosynthesis*; D014158:Transcription, Genetic; D051816:Tristetraprolin; D016335:Zinc Fingers; D015053:Zygote / Q000378:metabolism
D004268:DNA-Binding Proteins; D017874:Immediate-Early Proteins; D011506:Proteins; D014157:Transcription Factors; D051816:Tristetraprolin