title: Ultraviolet A radiation (320-400 nm) protects hairless mice from immunosuppression induced by ultraviolet B radiation (280-320 nm) or cis-urocanic acid.
abstract: T cell-mediated immune function, here measured as the contact hypersensitivity reaction, is readily suppressed by moderate exposure of mice to ultraviolet B (UVB) or solar-simulated radiation (SSUV), or by topical application of cis-urocanic acid. The effect of ultraviolet A (UVA) radiation on immune function has been unclear. Here we have demonstrated that when UVA radiation from a fluorescent tube source was rigorously filtered to remove contaminating UVB radiation, it was immunologically innocuous at physiologically relevant doses. Furthermore, we have found that mice exposed to UVA radiation, either immediately after, or up to 24 h before, immunosuppressive treatment with either UVB radiation, SSUV or cis-urocanic acid, became refractory to the immunosuppression and retained more normal contact hypersensitivity. A greater UVA exposure reversed the immunosuppression more effectively. The results suggest that there are immunologically significant interactions between UV wavebands, and that UVA exposure may induce a relatively long-lived immunoprotective photoproduct, as yet unidentified, that can inhibit the activity of epidermal cis-urocanic acid and thus provide protection from photoimmunosuppression.
authors.affiliation: Department of Veterinary Pathology, University of Sydney, NSW, Australia. v.reeve@vetp.usyd.edu.au
authors.forename: V E
authors.initials: VE
authors.lastname: Reeve
authors.forename: M
authors.initials: M
authors.lastname: Bosnic
authors.forename: C
authors.initials: C
authors.lastname: Boehm-Wilcox
authors.forename: N
authors.initials: N
authors.lastname: Nishimura
authors.forename: R D
authors.initials: RD
authors.lastname: Ley
mesh_terms: D000818:Animals; D003877:Dermatitis, Contact / Q000276:immunology*; D005260:Female; D007107:Immune System / Q000187:drug effects / Q000528:radiation effects*; D051379:Mice; D008812:Mice, Hairless; D012867:Skin / Q000276:immunology / Q000528:radiation effects*; D013601:T-Lymphocytes / Q000187:drug effects / Q000276:immunology* / Q000528:radiation effects; D014466:Ultraviolet Rays; D014560:Urocanic Acid / Q000633:toxicity*
chemical_list: D014560:Urocanic Acid