title: G-protein-mediated signal transduction for meiosis reinitiation in starfish oocyte.
abstract: Starfish oocyte maturation is induced by 1-methyladenine. There were apparently two forms of 1-methyladenine receptor affected by GTP gamma S. Pertussis toxin ADP-ribosylated the 39-kDa alpha subunit of a G protein and inhibited maturation. Also, the G protein was ADP-ribosylated by cholera toxin only when 1-methyladenine was added. The purified G protein had an heterotrimeric structure consisting of 39 kDa alpha, 37 kDa beta, and 8 kDa gamma subunits. The deduced amino acid sequence of the cDNA of starfish G alpha was 89% identical to mammalian Gi-1 alpha. The purified starfish beta gamma-subunits induced maturation when they were microinjected into oocytes.
authors.affiliation: Department of Life Science, Tokyo Institute of Technology, Yokohama, Japan.
authors.forename: K
authors.initials: K
authors.lastname: Chiba
authors.forename: M
authors.initials: M
authors.lastname: Hoshi
mesh_terms: D000246:Adenosine Diphosphate Ribose / Q000378:metabolism; D000595:Amino Acid Sequence; D000818:Animals; D002462:Cell Membrane / Q000378:metabolism; D019204:GTP-Binding Proteins / Q000502:physiology*; D008540:Meiosis; D008969:Molecular Sequence Data; D009865:Oocytes / Q000378:metabolism / Q000502:physiology; D037342:Pertussis Toxin; D015398:Signal Transduction; D013215:Starfish / Q000502:physiology; D010566:Virulence Factors, Bordetella / Q000494:pharmacology
chemical_list: D010566:Virulence Factors, Bordetella; D000246:Adenosine Diphosphate Ribose; D037342:Pertussis Toxin; D019204:GTP-Binding Proteins