title: Sequence and phylogenetic analysis of the Borrelia burgdorferi secA gene.
abstract: A Borrelia burgdorferi secA homologue was cloned and the complete DNA sequence was determined. The deduced protein sequence consists of 899 amino acids and shows a high degree of homology to SecA homologues from other Bacteria and photosynthetic plastids. The presence of the secA gene in Spirochetes suggests that this gene is present in most if not all major lineages within Bacteria. The ease of isolation of secA by conservation of its ATP-binding motifs combined with its extreme conservation in protein secretion pathways and the presence of a phylogenetic sequence marker in one of its ATP-binding domains makes this gene useful for phylogenetic analysis of Bacteria and photosynthetic plastids.
authors.affiliation: Department of Molecular Biology and Biochemistry, Wesleyan University, Middletown, CT 06459, USA.
authors.forename: T
authors.initials: T
authors.lastname: Guina
authors.forename: D
authors.initials: D
authors.lastname: Helfet-Hilliker
authors.forename: V
authors.initials: V
authors.lastname: Ramamurthy
authors.forename: D
authors.initials: D
authors.lastname: Oliver
mesh_terms: D000251:Adenosine Triphosphatases / Q000235:genetics*; D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000235:genetics*; D001483:Base Sequence; D015748:Borrelia burgdorferi Group / Q000235:genetics*; D003001:Cloning, Molecular; D004269:DNA, Bacterial / Q000235:genetics; D029968:Escherichia coli Proteins; D005798:Genes, Bacterial / Q000235:genetics*; D026901:Membrane Transport Proteins; D008969:Molecular Sequence Data; D010802:Phylogeny; D000069816:SEC Translocation Channels; D000081416:SecA Proteins; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid
chemical_list: D001426:Bacterial Proteins; D004269:DNA, Bacterial; D029968:Escherichia coli Proteins; D026901:Membrane Transport Proteins; D000069816:SEC Translocation Channels; D000251:Adenosine Triphosphatases; D000081416:SecA Proteins