Cytotoxicity and DNA binding characteristics of dextran-conjugated doxorubicins.
The antitumor antibiotic doxorubicin was conjugated with polymeric dextrans of various molecular weights and the cytotoxicity of the conjugates against human carcinoma KB-3-1 cells and its multidrug-resistant subclone KB-V-1 cells was measured by tetrazolium salt MTT assay. The conjugates were much less toxic to the KB-3-1 cells than the free doxorubicin but exhibited similar toxicity to the KB-V-1 cells. The conjugate-DNA interactions were monitored in real-time using an optical biosensor based on evanescent wave detection to obtain the association (ka) and dissociation (kd) rate constants as well as the equilibrium binding constants (KA) of the bindings. Both ka and kd values for the conjugates are more than three magnitudes smaller than those for free doxorubicin, while the KA values of the conjugate-DNA complexes are only about 10 times smaller than that of the free doxorubicin-DNA complex. The results indicate that the cytotoxicity and the DNA-binding kinetics of doxorubicin may be modified with dextran conjugation. The KA values obtained from the biosensor measurements were in close agreement with those determined in solution by fluorescent titration method, verifying the utility of the label-free biosensing measurements as an efficient method for studying ligand-DNA interactions.
Yang
M
M
Department of Biology and Chemistry, City University of Hong Kong, 83 Tat Chee Avenue, Kowloon, Hong Kong. bhmyang@cityu.edu.hk
Chan
H L
HL
Lam
W
W
Fong
W F
WF
D001665:Binding Sites / Q000187:drug effects; D015374:Biosensing Techniques; D004273:DNA, Neoplasm / Q000187:drug effects / Q000378:metabolism*; D003911:Dextrans / Q000378:metabolism / Q000633:toxicity; D004317:Doxorubicin / Q000031:analogs & derivatives / Q000378:metabolism* / Q000633:toxicity*; D018432:Drug Resistance, Multiple; D019008:Drug Resistance, Neoplasm; D006801:Humans; D007624:KB Cells; D007700:Kinetics; D013050:Spectrometry, Fluorescence; D013816:Thermodynamics
D004273:DNA, Neoplasm; D003911:Dextrans; D004317:Doxorubicin