title: The organ-maintained human sebaceous gland.
abstract: In the absence of epidermal growth factor (EGF) and phenol red in the maintenance medium, human sebaceous glands apparently retain in vivo rates of cell division and lipogenesis over 7 days and also retain in situ morphology. This is reversible on the re-addition of 10 ng/ml EGF and 10 mg/ml phenol red. The addition of 600 pM 17 beta-oestradiol results in a significant fall in the rate of lipogenesis over 7 days' maintenance, without affecting the rate of cell division. This effect is apparently due to abnormal differentiation of newly formed sebocytes. Neither 1 nM testosterone nor 1 nM dihydrotestosterone has any effect on rates of cell division or lipogenesis over 7 days. 1 microM 13-cis-retinoic acid causes a significant reduction in the rate of lipogenesis over 7 days.
authors.affiliation: Department of Clinical Biochemistry, University of Cambridge, Addenbrooke's Hospital, UK.
authors.forename: R
authors.initials: R
authors.lastname: Guy
authors.forename: T
authors.initials: T
authors.lastname: Kealey
mesh_terms: D000328:Adult; D000368:Aged; D002455:Cell Division / Q000187:drug effects; D003470:Culture Media; D046508:Culture Techniques; D004247:DNA / Q000096:biosynthesis; D013196:Dihydrotestosterone / Q000494:pharmacology; D004815:Epidermal Growth Factor / Q000494:pharmacology; D004958:Estradiol / Q000494:pharmacology; D006801:Humans; D015474:Isotretinoin / Q000494:pharmacology; D008055:Lipids / Q000096:biosynthesis; D008297:Male; D008875:Middle Aged; D010637:Phenolsulfonphthalein / Q000494:pharmacology; D012627:Sebaceous Glands / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism*; D013739:Testosterone / Q000494:pharmacology
chemical_list: D003470:Culture Media; D008055:Lipids; D013196:Dihydrotestosterone; D013739:Testosterone; D004958:Estradiol; D004815:Epidermal Growth Factor; D004247:DNA; D015474:Isotretinoin; D010637:Phenolsulfonphthalein