Trapidil effects on intimal thickening and mRNA levels for platelet-derived growth factor A in human saphenous vein smooth muscle cells.
BACKGROUND
Platelet-derived growth factor (PDGF) A chain, basic fibroblast growth factor (bFGF), and tissue-type plasminogen activator (tPA) from smooth muscle cells (SMCs) have been postulated to initiate SMC proliferation and migration in the process of intimal thickening.


OBJECTIVE
In this study we tested whether trapidil, which is reported to reduce intimal thickening, would suppress mRNA increases for all or only some of these genes in human SMC.


METHODS
Cultured human saphenous vein SMCs made quiescent by 72-hour incubation in 0.5% serum were stimulated with 3U/mL alpha-thrombin +/- trapidil. Changes in mRNA transcript levels were analyzed by Northern blot.


RESULTS
Trapidil decreased thrombin-induced mRNA levels for bFGF and appeared to decrease mRNA for PDGF-A chain, but not for tPA.


CONCLUSIONS
These results suggest that trapidil may reduce intimal thickening at least partly via the suppression of increased bFGF and PDGF-A chain mRNA levels in vascular SMCs.
Yamaguchi
M
M
Department of Surgery, Show University Fujigaoka Hospitals, Yokohama, Japan.
Kumada
K
K
D001345:Autoradiography; D015152:Blotting, Northern; D016222:Fibroblast Growth Factor 2 / Q000096:biosynthesis; D006801:Humans; D009131:Muscle, Smooth, Vascular / Q000187:drug effects*; D010975:Platelet Aggregation Inhibitors / Q000494:pharmacology*; D010982:Platelet-Derived Growth Factor / Q000096:biosynthesis* / Q000235:genetics; D012333:RNA, Messenger / Q000378:metabolism; D012501:Saphenous Vein / Q000187:drug effects*; D014192:Trapidil / Q000494:pharmacology*; D017539:Tunica Intima / Q000187:drug effects*
D010975:Platelet Aggregation Inhibitors; D010982:Platelet-Derived Growth Factor; D012333:RNA, Messenger; D016222:Fibroblast Growth Factor 2; D014192:Trapidil