Preliminary report on fractionation of fucans by ion-exchange displacement centrifugal partition chromatography.
A new method combining ion-exchange displacement chromatography with centrifugal partition chromatography (CPC) was used for the fractionation of partially depolymerized fucans (polysulphated polysaccharides). The ion-exchanger was Amberlite LA2, a high-molecular-mass liquid secondary amine miscible with most common organic solvents and immiscible with aqueous solutions. Ion-exchange displacement centrifugal partition chromatography was performed with LA2 in methyl isobutyl ketone (MiBK) as the stationary phase, water as the mobile phase, Cl- as the carrier and OH- as the displacer. A complex mixture of partially depolymerized fucans was resolved into adjacent families characterized by their peak molecular mass and polydispersity. The Dubois test (sugar) and the azur A test (SO3-) confirmed the displacement mode of the process, and size-exclusion chromatographic controls confirmed its efficiency.
Chevolot
L
L
URM2-CNRS URA 502, Laboratoire de recherche sur les macromolécules, Université Paris Nord, Villetaneuse, France.
Colliec-Jouault
S
S
Foucault
A
A
Ratiskol
J
J
Sinquin
C
C
D005591:Chemical Fractionation; D002852:Chromatography, Ion Exchange / Q000379:methods*; D006863:Hydrogen-Ion Concentration; D011134:Polysaccharides / Q000032:analysis / Q000302:isolation & purification*; D012624:Seaweed / Q000737:chemistry; D013431:Sulfates / Q000032:analysis
D011134:Polysaccharides; D013431:Sulfates; C007789:fucoidan