title: Comparative analysis of MiB1 and p53 expression in human bladder tumors and their correlation with cancer progression.
abstract: Expression of p53 and MiB1, markers of tumor proliferation, was evaluated in human bladder tumors, and correlated with ploidy and cancer progression in 83 consecutive patients. Transurethral resection of a newly diagnosed bladder tumor was performed in 73 cases, and systematic bladder biopsies were performed in 10 cases after bacillus Calmette-Guerin (BCG) treatment. p53 and MiB1 expression were performed by an immunohistochemical technique and the ploidy was determined on a frozen fragment of the tumor. p53 expression was correlated in relation to grade, stage and combination of grade and stage. MiB1 expression was correlated with cytological grade, and a significant difference was demonstrated between pT0 and pTa, pTa, and pT1, pTa and pT2 tumors but not between pT1 and > or = pT2 tumors. A discordance was observed for the comparison of p53 and MiB1 values, stage by stage, suggesting that these two techniques are independent of each other. A larger proportion of aneuploid tumors were positive for p53 and MiB1 (64.8 vs. 86.5%, respectively), but p53 and MiB1 immunostaining were not better indicators than ploidy alone to predict cancer progression.
authors.affiliation: Clinique Urologique, Centre Hospitalo-Universitaire, Hôtel Dieu, Nantes, France.
authors.forename: C
authors.initials: C
authors.lastname: Pfister
authors.forename: F
authors.initials: F
authors.lastname: Buzelin
authors.forename: C
authors.initials: C
authors.lastname: Casse
authors.forename: G
authors.initials: G
authors.lastname: Bochereau
authors.forename: J M
authors.initials: JM
authors.lastname: Buzelin
authors.forename: O
authors.initials: O
authors.lastname: Bouchot
mesh_terms: D000911:Antibodies, Monoclonal; D014408:Biomarkers, Tumor / Q000032:analysis; D004273:DNA, Neoplasm / Q000032:analysis; D006801:Humans; D007150:Immunohistochemistry; D019394:Ki-67 Antigen / Q000032:analysis*; D011003:Ploidies; D011379:Prognosis; D016159:Tumor Suppressor Protein p53 / Q000032:analysis* / Q000235:genetics; D001749:Urinary Bladder Neoplasms / Q000737:chemistry / Q000235:genetics / Q000473:pathology*
chemical_list: D000911:Antibodies, Monoclonal; D014408:Biomarkers, Tumor; D004273:DNA, Neoplasm; D019394:Ki-67 Antigen; D016159:Tumor Suppressor Protein p53