Distinct patterns of folding and interactions with calnexin and calreticulin in human class I MHC proteins with altered N-glycosylation.
Calnexin is a lectin-like chaperone that binds to class I MHC molecules soon after their synthesis, retaining unassembled heavy chains and also assisting their folding. Following association with beta2-microglobulin (beta2m) in the endoplasmic reticulum, a large proportion of human class I molecules release from calnexin, whereas mouse class I molecules do not. We asked whether addition of a second N-glycan to the human class I molecule A*0201 at position 176, a site present in mouse, would affect its binding to calnexin. The 176dg mutant with N-glycans at positions 86 and 176, when transfected into CIR cells, demonstrated increased binding to calnexin, detectable both before and after association with beta2m, and reduced interaction with calreticulin and TAP relative to wild-type protein bearing a single N-glycan at position 86. Cell surface levels of the mutant were decreased only slightly relative to the wild type, suggesting that the protein is not misfolded or grossly altered structurally. A subpopulation of mutant molecules was retained in the endoplasmic reticulum, and surprisingly, these molecules reacted with w6/32, which recognizes an epitope present on transport-competent class I HLA complexes. Transfection into Daudi cells demonstrated that 176dg reacts with w6/32 in the absence of beta2m, suggesting that the Ab epitope can be induced by binding of calnexin. These data may explain previously noted differences between mouse and human class I MHC proteins and demonstrate that the location of N-oligosaccharides within proteins can influence their folding and interactions with chaperones such as calnexin and calreticulin.
Zhang
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Department of Pathology and University of Pittsburgh Cancer Institute, University of Pittsburgh School of Medicine, PA 15213, USA.
Salter
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D000483:Alleles; D001692:Biological Transport / Q000235:genetics / Q000276:immunology; D002135:Calcium-Binding Proteins / Q000378:metabolism*; D037281:Calnexin; D037282:Calreticulin; D002460:Cell Line; D002462:Cell Membrane / Q000235:genetics / Q000276:immunology / Q000378:metabolism; D005947:Glucose / Q000378:metabolism; D006031:Glycosylation; D015234:HLA-A Antigens / Q000096:biosynthesis / Q000235:genetics / Q000378:metabolism*; D006801:Humans; D008565:Membrane Proteins / Q000378:metabolism; D018448:Models, Immunological; D016297:Mutagenesis, Site-Directed; D011134:Polysaccharides / Q000378:metabolism; D011485:Protein Binding / Q000235:genetics / Q000276:immunology; D017510:Protein Folding; D012261:Ribonucleoproteins / Q000378:metabolism*; D001613:beta 2-Microglobulin / Q000378:metabolism
D002135:Calcium-Binding Proteins; D037282:Calreticulin; D015234:HLA-A Antigens; D008565:Membrane Proteins; D011134:Polysaccharides; D012261:Ribonucleoproteins; D001613:beta 2-Microglobulin; D037281:Calnexin; D005947:Glucose