Sonodynamically-induced in vitro cell damage enhanced by adriamycin.
Sonodynamically-induced cell damage and active oxygen generation enhanced by adriamycin (ADM) were compared in the same in vitro insonation set-up. Significant enhancement of the rates of both ultrasonically-induced cell damage and nitroxide generation was demonstrated with 40-160 microM ADM. Both rates correlated very well resulting in a correlation coefficient of more than 0.99. The enhancement of both rates was suppressed by 10 mM histidine. These results are consistent with the hypothesis that ultrasonically-generated active oxygen plays a major role in the sonodynamically-induced cell damage enhanced by ADM.
Umemura
S
S
Central Research Laboratory, Hitachi, Ltd., Kokubunnji, Tokyo, Japan.
Yumita
N
N
Okano
Y
Y
Kaneuchi
M
M
Magario
N
N
Ishizaki
M
M
Shimizu
K
K
Sano
Y
Y
Umemura
K
K
Nishigaki
R
R
D000818:Animals; D000903:Antibiotics, Antineoplastic / Q000627:therapeutic use*; D002470:Cell Survival / Q000502:physiology; D003131:Combined Modality Therapy; D004317:Doxorubicin / Q000627:therapeutic use*; D004578:Electron Spin Resonance Spectroscopy; D016166:Free Radical Scavengers / Q000494:pharmacology; D016014:Linear Models; D008297:Male; D051379:Mice; D008813:Mice, Inbred ICR; D009589:Nitrogen Oxides / Q000378:metabolism; D010100:Oxygen / Q000378:metabolism*; D017382:Reactive Oxygen Species / Q000378:metabolism; D012510:Sarcoma 180 / Q000378:metabolism / Q000473:pathology / Q000628:therapy*; D014407:Tumor Cells, Cultured; D014464:Ultrasonic Therapy / Q000379:methods*
D000903:Antibiotics, Antineoplastic; D016166:Free Radical Scavengers; D009589:Nitrogen Oxides; D017382:Reactive Oxygen Species; D004317:Doxorubicin; D010100:Oxygen