Effects of a urinary factor from women in early pregnancy on HIV-1, SIV and associated disease.
The effects of clinical grade crude preparations of human chorionic gonadotropin (hCG) on Kaposi's sarcoma, HIV, SIV and hematopoiesis were examined in vitro and in vivo. In contrast to previous studies, we report that the antiviral activity of hCG associated factors is not due to the native hCG heterodimer, including its purified subunits or its major degradation product, the beta-core. Using gel permeation chromatography of the clinical grade hCG and urine concentrates from pregnant women, we demonstrate that an as yet unidentified hCG associated factor (HAF) with anti-HIV, anti-SIV, anti-KS and pro-hematopoietic activities elutes as two peaks corresponding to 15-30 kDa and 2-4 kDa.
Lunardi-Iskandar
Y
Y
Institute of Human Virology, University of Maryland, Baltimore 21201-1192, USA.
Bryant
J L
JL
Blattner
W A
WA
Hung
C L
CL
Flamand
L
L
Gill
P
P
Hermans
P
P
Birken
S
S
Gallo
R C
RC
D000818:Animals; D000998:Antiviral Agents / Q000302:isolation & purification / Q000494:pharmacology / Q000627:therapeutic use / Q000652:urine*; D001685:Biological Factors / Q000302:isolation & purification / Q000494:pharmacology* / Q000627:therapeutic use / Q000652:urine*; D002470:Cell Survival / Q000187:drug effects; D006063:Chorionic Gonadotropin / Q000302:isolation & purification / Q000494:pharmacology / Q000652:urine*; D019281:Dimerization; D005260:Female; D017353:Gene Deletion; D015750:Genes, gag; D015751:Genes, pol; D016679:Genome, Viral; D015497:HIV-1 / Q000187:drug effects / Q000235:genetics / Q000502:physiology*; D006801:Humans; D008253:Macaca mulatta; D008297:Male; D051379:Mice; D008822:Mice, Transgenic; D011247:Pregnancy / Q000652:urine*; D012514:Sarcoma, Kaposi; D016097:Simian Acquired Immunodeficiency Syndrome / Q000188:drug therapy*; D014407:Tumor Cells, Cultured; D014410:Tumor Stem Cell Assay; D014779:Virus Replication / Q000187:drug effects*
D000998:Antiviral Agents; D001685:Biological Factors; D006063:Chorionic Gonadotropin