title: Chemical imaging of nucleic acids, proteins and lipids of a single living cell. Application of synchrotron infrared microspectrometry in cell biology.
abstract: Hybridoma B-cells have been used as models to evaluate the performance of synchrotron infrared microscopy to obtain chemical images of a single living cell. Chemical mapping of nucleic acids, proteins and lipids at a resolution of a few microns, close to the diffraction limit in the mid-infrared region are shown.
authors.affiliation: CEA-INSTN-CIE, Gif-Sur-Yvette, France.
authors.forename: N
authors.initials: N
authors.lastname: Jamin
authors.forename: P
authors.initials: P
authors.lastname: Dumas
authors.forename: J
authors.initials: J
authors.lastname: Moncuit
authors.forename: W H
authors.initials: WH
authors.lastname: Fridman
authors.forename: J L
authors.initials: JL
authors.lastname: Teillaud
authors.forename: G L
authors.initials: GL
authors.lastname: Carr
authors.forename: G P
authors.initials: GP
authors.lastname: Williams
mesh_terms: D000818:Animals; D008055:Lipids / Q000032:analysis*; D051379:Mice; D009696:Nucleic Acids / Q000032:analysis*; D011506:Proteins / Q000032:analysis*; D013055:Spectrophotometry, Infrared / Q000379:methods*; D017356:Synchrotrons; D014407:Tumor Cells, Cultured
chemical_list: D008055:Lipids; D009696:Nucleic Acids; D011506:Proteins