Leukocyte adhesion molecule expression during intense resistance exercise.
We hypothesized that expression of L-selectin and very late antigen-4 (VLA-4) integrin adhesion molecules would influence cell type-specific redistribution during exercise. Women subjects performed six sets of 10-repetition maximum squats. L-selectin and VLA-4 integrin were measured by using flow cytometry pre- and postexercise on peripheral blood neutrophils and lymphocytes (n = 29 subjects) and lymphocyte subsets (n = 70 subjects), respectively. Neutrophil concentration increased 41.8% (P < 0.001), whereas the percent expressing L-selectin was unchanged (79%). Lymphocyte concentration increased 61.8% (P < 0.001). The percent of T cells expressing L-selectin decreased from 73.5 +/- 8.9 to 68.2 +/- 11.4% (P < 0.001); the combined population of natural killer and B cells expressing L-selectin decreased from 80.4 +/- 22.5 to 62.7 +/- 25.8% (P < 0.001). VLA-4 integrin was expressed by nearly all lymphocytes both pre- and postexercise. The proportional decrease in L-selectin positive cells could have resulted from 1) shedding of L-selectin, 2) selective entry of L-selectin-negative subsets, or 3) selective removal of L-selectin-positive subsets.
Miles
M P
MP
Department of Biochemistry and Molecular Biology, Pennsylvania State University, University Park 16802, USA.
Leach
S K
SK
Kraemer
W J
WJ
Dohi
K
K
Bush
J A
JA
Mastro
A M
AM
D000328:Adult; D015815:Cell Adhesion Molecules / Q000378:metabolism*; D005260:Female; D005434:Flow Cytometry; D006801:Humans; D039041:Integrin alpha4beta1; D016023:Integrins / Q000378:metabolism; D019041:L-Selectin / Q000378:metabolism; D007958:Leukocyte Count; D007962:Leukocytes / Q000502:physiology*; D008214:Lymphocytes / Q000502:physiology; D008297:Male; D009504:Neutrophils / Q000502:physiology; D005082:Physical Exertion / Q000502:physiology*; D016201:Receptors, Lymphocyte Homing / Q000378:metabolism
D015815:Cell Adhesion Molecules; D039041:Integrin alpha4beta1; D016023:Integrins; D016201:Receptors, Lymphocyte Homing; D019041:L-Selectin