title: A new Drosophila ultraviolet light-damaged DNA recognition endonuclease that selectively nicks a (6-4) photoproduct site.
abstract: We have previously described the purification of an ultraviolet light (UV) damage-specific DNA-binding protein from Drosophila melanogaster, designated D-DDB P1 [Nucleic Acids Res., 23 (1995) 2600-2607]. Here, we obtained highly purified D-DDB P1 from Drosophila Kc cells, and we found that D-DDB P1 is also a nuclease. D-DDB P1 can selectively bind to pyrimidine (6-4) pyrimidone photoproducts, and in the presence of Mg++, D-DDB P1 can catalyze an incision immediately on the 3' and 5' sides of the (6-4) photoproduct site.
authors.affiliation: Department of Applied Biological Science, Faculty of Science and Technology, Science University of Tokyo, 2641 Yamazaki,Noda-shi, Chiba-ken 278, Japan.
authors.forename: M
authors.initials: M
authors.lastname: Kai
authors.forename: T
authors.initials: T
authors.lastname: Todo
authors.forename: M
authors.initials: M
authors.lastname: Wada
authors.forename: H
authors.initials: H
authors.lastname: Ryo
authors.forename: C
authors.initials: C
authors.lastname: Masutani
authors.forename: H
authors.initials: H
authors.lastname: Kobayashi
authors.forename: H
authors.initials: H
authors.lastname: Morioka
authors.forename: E
authors.initials: E
authors.lastname: Ohtsuka
authors.forename: F
authors.initials: F
authors.lastname: Hanaoka
authors.forename: K
authors.initials: K
authors.lastname: Sakaguchi
mesh_terms: D000818:Animals; D001483:Base Sequence; D004249:DNA Damage; D004260:DNA Repair; D004269:DNA, Bacterial / Q000528:radiation effects*; D004268:DNA-Binding Proteins / Q000235:genetics*; D029721:Drosophila Proteins; D004331:Drosophila melanogaster / Q000201:enzymology / Q000235:genetics*; D004706:Endodeoxyribonucleases / Q000302:isolation & purification*; D008969:Molecular Sequence Data; D014466:Ultraviolet Rays
chemical_list: D004269:DNA, Bacterial; D004268:DNA-Binding Proteins; D029721:Drosophila Proteins; D004706:Endodeoxyribonucleases; C112206:DdbP1 protein, Drosophila