Ovalbumin coupled either with murine red blood cells or liposome induces IgG but not IgE antibody production.
Ovalbumin (OVA) was coupled with murine red blood cells (MRBC) using glutaraldehyde. The OVA-MRBC conjugate induced anti-OVA IgG antibody in mice at almost the same level as OVA in alum. However, no IgE antibody production specific for OVA was observed in OVA-MRBC-injected mice. A significant increase in IGG2a production was obtained with OVA-MRBC immunization, whereas the production of IgG1 predominated in OVA in alum immunization. Am OVA-liposome conjugate induced IgE-specific unresponsiveness in mice in the same manner as OVA-MRBC. Similar results were obtained when antigens other than OVA, such as tetanus toxoid or diphtheria toxoid, were coupled to liposome. These results show the potential of antigen-liposome conjugates for the development of vaccine that induces sufficient IgG antibody production without IgE synthesis.
Uchida
T
T
Dept. of Safety Research on Biologics, National Inst. of Infectious Diseases, Tokyo, Japan.
Naito
S
S
Horino
A
A
Taneichi
M
M
Mizuguchi
J
J
Nakano
Y
Y
Oka
T
T
Ookuma
K
K
Morokuma
K
K
Sakurai
S
S
Komuro
K
K
D000534:Alum Compounds; D000818:Animals; D004912:Erythrocytes / Q000276:immunology*; D005260:Female; D007114:Immunization / Q000379:methods; D007073:Immunoglobulin E / Q000096:biosynthesis*; D007074:Immunoglobulin G / Q000096:biosynthesis*; D008081:Liposomes; D051379:Mice; D008807:Mice, Inbred BALB C; D010047:Ovalbumin / Q000276:immunology*; D051381:Rats; D017207:Rats, Sprague-Dawley
D000534:Alum Compounds; D007074:Immunoglobulin G; D008081:Liposomes; C041524:aluminum sulfate; D007073:Immunoglobulin E; D010047:Ovalbumin