Phospholamban domain I/cytochrome b5 transmembrane sequence chimeras do not inhibit SERCA2a.
A series of chimeras between the transmembrane domains of phospholamban (PLN) and cytochrome b5 were coexpressed with the Ca2+-ATPase of cardiac sarcoplasmic reticulum (SERCA2a). The chimeric molecules were not inhibitory, in line with our view that inhibitory PLN/SERCA2a interactions occur in transmembrane sequences, while cytoplasmic interactions regulate the inhibitory interactions in a four-base circuit.
Kimura
Y
Y
Banting and Best Department of Medical Research, University of Toronto, Charles H. Best Institute, Ont., Canada.
Asahi
M
M
Kurzydlowski
K
K
Tada
M
M
MacLennan
D H
DH
D000818:Animals; D002118:Calcium / Q000378:metabolism; D002135:Calcium-Binding Proteins / Q000235:genetics* / Q000378:metabolism / Q000502:physiology; D000252:Calcium-Transporting ATPases / Q000378:metabolism*; D002460:Cell Line; D015786:Cytochromes b5 / Q000235:genetics* / Q000378:metabolism; D005455:Fluorescent Antibody Technique; D015870:Gene Expression / Q000235:genetics; D006801:Humans; D007150:Immunohistochemistry; D008861:Microsomes / Q000378:metabolism; D009206:Myocardium / Q000737:chemistry; D010766:Phosphorylation; D011817:Rabbits; D011993:Recombinant Fusion Proteins / Q000235:genetics* / Q000494:pharmacology; D012519:Sarcoplasmic Reticulum / Q000201:enzymology; D000099211:Phospholamban
D002118:Calcium; D002135:Calcium-Binding Proteins; D000252:Calcium-Transporting ATPases; D015786:Cytochromes b5; D011993:Recombinant Fusion Proteins; D000099211:Phospholamban