title: Phosphorylation of elongation factor-1 (EF-1) by cdc2 kinase.
abstract: Elongation factor-1 (EF-1) is a major substrate for cdc2 kinase in Xenopus oocytes. The guanine-nucleotide exchange factor EF-1 beta gamma delta, appears to have a highly complex macromolecular structure containing several GTP/GDP exchange proteins, valyl-tRNA synthetase, and a putative anchoring protein EF-1 gamma. During meiotic cell division, the factor becomes phosphorylated by cdc2 kinase, not only on EF-1 gamma, but also on two different phospho-acceptors on EF-1 delta. Phosphorylation is concomitant with changes in protein synthesis in vivo. Xenopus oocytes, and potentially all cells, contain a multitude of heteromeric forms of the complex which postulates that EF-1 beta gamma delta is not a "house keeping" factor but a sophisticated regulatory element.
authors.affiliation: Biologie Cellulaire de l'Ovocyte, CNRS URA 1449, INRA, Université Pierre et Marie Curie, Paris, France.
authors.forename: R
authors.initials: R
authors.lastname: Bellé
authors.forename: O
authors.initials: O
authors.lastname: Minella
authors.forename: P
authors.initials: P
authors.lastname: Cormier
authors.forename: J
authors.initials: J
authors.lastname: Morales
authors.forename: R
authors.initials: R
authors.lastname: Poulhe
authors.forename: O
authors.initials: O
authors.lastname: Mulner-Lorillon
mesh_terms: D000818:Animals; D016203:CDC2 Protein Kinase / Q000502:physiology*; D008540:Meiosis; D009865:Oocytes; D020648:Peptide Elongation Factor 1; D010445:Peptide Elongation Factors / Q000378:metabolism*; D010766:Phosphorylation; D014981:Xenopus
chemical_list: D020648:Peptide Elongation Factor 1; D010445:Peptide Elongation Factors; D016203:CDC2 Protein Kinase