Comparative gene expression profiling by oligonucleotide fingerprinting.
The use of hybridisation of synthetic oligonucleotides to cDNAs under high stringency to characterise gene sequences has been demonstrated by a number of groups. We have used two cDNA libraries of 9 and 12 day mouse embryos (24 133 and 34 783 clones respectively) in a pilot study to characterise expressed genes by hybridisation with 110 hybridisation probes. We have identified 33 369 clusters of cDNA clones, that ranged in representation from 1 to 487 copies (0.7%). 737 were assigned to known rodent genes, and a further 13 845 showed significant homologies. A total of 404 clusters were identified as significantly differentially represented (P < 0.01) between the two cDNA libraries. This study demonstrates the utility of the fingerprinting approach for the generation of comparative gene expression profiles through the analysis of cDNAs derived from different biological materials.
Meier-Ewert
S
S
Max-Planck-Institut für Molekulare Genetik, Ihnestrasse 73, 14195 Berlin, Germany. sebastian.meier-ewert@gpc-ag.com
Lange
J
J
Gerst
H
H
Herwig
R
R
Schmitt
A
A
Freund
J
J
Elge
T
T
Mott
R
R
Herrmann
B
B
Lehrach
H
H
D000818:Animals; D018076:DNA, Complementary / Q000235:genetics*; D016208:Databases, Factual; D005314:Embryonic and Fetal Development / Q000235:genetics*; D015870:Gene Expression; D015723:Gene Library; D051379:Mice; D009693:Nucleic Acid Hybridization / Q000379:methods*; D015345:Oligonucleotide Probes; D010865:Pilot Projects; D016133:Polymerase Chain Reaction
D018076:DNA, Complementary; D015345:Oligonucleotide Probes