Identification of elf1, a beta-spectrin, in early mouse liver development.
Beta-spectrins play essential roles in cell-cell interactions and in the maintenance of cell polarity. Our aim was to identify beta-spectrin genes important for the establishment of hepatocyte polarity and differentiation. Using subtractive screening of cDNA libraries from early embryonic mouse livers (post-coital days 10, 11, and 12), we have isolated elf1 (embryonic liver fodrin 1), a differentially expressed beta-spectrin or fodrin (betaSpIIsigmaI). Elf1 encodes a 220-amino acid protein with an NH2 terminal actin-binding domain. In situ hybridization studies demonstrate elf1 expression initially in day 10 embryonic heart tissue, then in day 11-11.5 hepatic tissue. These studies suggest that elf1 may play a role in the emergence of hepatocyte polarity during liver development.
Mishra
L
L
Department of Medicine, Department of Veterans Affairs Medical Center, Washington, DC 20422, USA. mishra.lopa@washington.va.gov
Cai
T
T
Levine
A
A
Weng
D
D
Mezey
E
E
Mishra
B
B
Gearhart
J
J
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002454:Cell Differentiation; D016764:Cell Polarity; D002999:Clone Cells; D018076:DNA, Complementary / Q000737:chemistry; D004268:DNA-Binding Proteins / Q000235:genetics / Q000302:isolation & purification*; D018507:Gene Expression Regulation, Developmental; D008099:Liver / Q000166:cytology / Q000196:embryology*; D051379:Mice; D008969:Molecular Sequence Data; D009687:Nuclear Proteins; D014157:Transcription Factors / Q000235:genetics / Q000302:isolation & purification*
D018076:DNA, Complementary; D004268:DNA-Binding Proteins; C512897:ELF1 protein, human; C512896:Elf1 protein, mouse; D009687:Nuclear Proteins; D014157:Transcription Factors