title: Vasal reanastomosis using fibrin glue combined with sutures: which combination of sutures in a delayed protocol? Experimental study in rats.
abstract: OBJECTIVE
  The actual number of transmural sutures needed to ensure a successful fibrin-glued vasovasostomy is a key study parameter of the few experimental works already published. The present work was done to evaluate fibrin-glued vasovasostomy in rats in combination with only 2 transmural sutures. We compared the results to our previous study in which we demonstrated the efficiency of a combination of the use of fibrin glue with 3 sutures in comparison with a conventional microsurgical technique.
  
  
  METHODS
  Twenty Sprague-Dawley rats underwent bilateral vasectomy followed 2 weeks later by bilateral vasovasostomy using fibrin glue combined with 2 transmural sutures. Each animal was sacrificed 7 weeks postoperatively after a 3-week mating period with a Sprague-Dawley female rat, the vasal specimens were evaluated for sperm granuloma formation. Mean operative time and fertility rates were recorded.
  
  
  RESULTS
  The combination of fibrin glue with 2 transmural sutures gave evidence of less successful performances than the combination with 3 transmural sutures and the conventional microsurgical technique for all parameters evaluated but the mean operative time.
  
  
  CONCLUSIONS
  Our study underlines the need for a third transmural suture placed 120 degrees apart from the others when performing a fibrin glue delayed vasovasostomy. This allows a better vas lumen opening at the anastomotic site and therefore a more efficient vasal anastomosis in a delayed protocol.
authors.affiliation: Clinique Urologique, Centre Hospitalier Universitaire, Lille, France.
authors.forename: O
authors.initials: O
authors.lastname: Vankemmel
authors.forename: A
authors.initials: A
authors.lastname: de la Taille
authors.forename: J M
authors.initials: JM
authors.lastname: Rigot
authors.forename: T
authors.initials: T
authors.lastname: Burnouf
authors.forename: E
authors.initials: E
authors.lastname: Mazeman
mesh_terms: D000714:Anastomosis, Surgical / Q000379:methods; D000818:Animals; D005260:Female; D005298:Fertility; D015718:Fibrin Tissue Adhesive; D008297:Male; D008866:Microsurgery; D011247:Pregnancy; D051381:Rats; D017207:Rats, Sprague-Dawley; D013536:Suture Techniques; D014659:Vasectomy; D014669:Vasovasostomy / Q000379:methods*
chemical_list: D015718:Fibrin Tissue Adhesive