title: 3' RACE: skewed ratio of specific to general PCR primers improves yield and specificity.
authors.affiliation: Mental Health Research Institute, University of Michigan, Ann Arbor 48109-0720, USA.
authors.forename: I N
authors.initials: IN
authors.lastname: Bespalova
authors.forename: S
authors.initials: S
authors.lastname: Adkins
authors.forename: M
authors.initials: M
authors.lastname: Burmeister
mesh_terms: D001921:Brain / Q000378:metabolism; D017931:DNA Primers / Q000235:genetics*; D018076:DNA, Complementary / Q000302:isolation & purification*; D004587:Electrophoresis, Agar Gel; D006801:Humans; D011071:Poly T / Q000235:genetics; D016133:Polymerase Chain Reaction / Q000379:methods*; D012313:RNA / Q000032:analysis; D012333:RNA, Messenger / Q000032:analysis*; D017422:Sequence Analysis, DNA; D019914:Taq Polymerase / Q000378:metabolism
chemical_list: D017931:DNA Primers; D018076:DNA, Complementary; D012333:RNA, Messenger; D011071:Poly T; D012313:RNA; D019914:Taq Polymerase