Non-detergent sulphobetaines enhance the recovery of membrane and/or cytoskeleton-associated proteins and active proteases from erythrocytes infected by Plasmodium falciparum.
A better understanding of the causative agent's biology and the definition of new targets for the development of drugs and/or specific immune responses is necessary to face the spred of drug-resistant malaria in developing countries and the absence of an efficient vaccine against this most important infectious disease. Non-detergent sulphobetaines enhance the recovery and isoelectric focussing of active Plasmodium falciparum proteases, cytoskeleton-associated proteins and Maurer's cleft-associated proteins. This is a significant advantage for the purification of such proteins and might help pinpoint their role for red blood cell rupture and merozoite release.
Blisnick
T
T
Unit of Experimental Parasitology, Institut Pasteur, Paris, France.
Morales-Betoulle
M E
ME
Vuillard
L
L
Rabilloud
T
T
Braun Breton
C
C
D000818:Animals; D001622:Betaine / Q000031:analogs & derivatives*; D001798:Blood Proteins / Q000302:isolation & purification*; D003598:Cytoskeletal Proteins / Q000097:blood / Q000302:isolation & purification*; D003599:Cytoskeleton / Q000469:parasitology / Q000502:physiology; D010450:Endopeptidases / Q000097:blood / Q000302:isolation & purification*; D004910:Erythrocyte Membrane / Q000469:parasitology / Q000502:physiology; D004912:Erythrocytes / Q000469:parasitology* / Q000502:physiology*; D007202:Indicators and Reagents; D007525:Isoelectric Focusing; D008565:Membrane Proteins / Q000097:blood / Q000302:isolation & purification*; D008970:Molecular Weight; D010963:Plasmodium falciparum / Q000472:pathogenicity / Q000502:physiology*; D015800:Protozoan Proteins / Q000097:blood / Q000302:isolation & purification*; D012995:Solubility
D001798:Blood Proteins; D003598:Cytoskeletal Proteins; D007202:Indicators and Reagents; D008565:Membrane Proteins; D015800:Protozoan Proteins; D001622:Betaine; D010450:Endopeptidases