A merozoite-specific 22-kDa rhoptry protein of the coccidium Eimeria nieschulzi (Sporozoa, Coccidia) is exocytosed in the parasitophorous vacuole upon host cell invasion.
A monoclonal antibody (Mab D12A4) was used to follow the genesis and fate of rhoptries from early first-generation merogony through second-generation merozoites of the rat coccidium Eimeria nieschulzi. The epitope recognized by Mab D12A4 belongs to a 22-kDa protein which can be localized first in developing meronts 25 h post-infection. Early rhoptries appear as distinct granules in the cytoplasm of developing meronts and elongate into mature organelles before merozoite release. The 22-kDa protein is found in the parasitophorous vacuole after host cell invasion. Western blotting and immunofluorescence showed that the 22-kDa rhoptry protein is expressed in schizonts and merozoites but not in sporozoites.
Rick
B
B
Institute of Zoology, University of Technology, Dresden, Germany.
Dubremetz
J F
JF
Entzeroth
R
R
D016475:3T3 Cells; D000818:Animals; D000911:Antibodies, Monoclonal; D000913:Antibodies, Protozoan; D015153:Blotting, Western; D002460:Cell Line; D004539:Eimeria / Q000254:growth & development / Q000378:metabolism / Q000502:physiology* / Q000648:ultrastructure; D005089:Exocytosis; D006790:Host-Parasite Interactions; D007422:Intestines / Q000166:cytology / Q000469:parasitology; D051379:Mice; D008854:Microscopy, Electron; D008858:Microscopy, Phase-Contrast; D015800:Protozoan Proteins / Q000032:analysis* / Q000276:immunology; D051381:Rats; D014617:Vacuoles / Q000737:chemistry* / Q000469:parasitology
D000911:Antibodies, Monoclonal; D000913:Antibodies, Protozoan; D015800:Protozoan Proteins