title: Inactivation of voltage-gated cardiac K+ channels.
abstract: Inactivation is the process by which an open channel enters a stable nonconducting conformation after a depolarizing change in membrane potential. Inactivation is a widespread property of many different types of voltage-gated ion channels. Recent advances in the molecular biology of K+ channels have elucidated two mechanistically distinct types of inactivation, N-type and C-type. N-type inactivation involves occlusion of the intracellular mouth of the pore through binding of a short segment of residues at the extreme N-terminal. In contrast to this "tethered ball" mechanism of N-type inactivation, C-type inactivation involves movement of conserved core domain residues that result in closure of the external mouth of the pore. Although C-type inactivation can show rapid kinetics that approach those observed for N-type inactivation, it is often thought of as a slowly developing and slowly recovering process. Current models of C-type inactivation also suggest that this process involves a relatively localized change in conformation of residues near the external mouth of the permeation pathway. The rate of C-type inactivation and recovery can be strongly influenced by other factors, such as N-type inactivation, drug binding, and changes in [K+]o. These interactions make C-type inactivation an important biophysical process in determining such physiologically important properties as refractoriness and drug binding. C-type inactivation is currently viewed as arising from small-scale rearrangements at the external mouth of the pore. This review will examine the multiplicity of interactions of C-type inactivation with N-terminal-mediated inactivation and drug binding that suggest that our current view of C-type inactivation is incomplete. This review will suggest that C-type inactivation must involve larger-scale movements of transmembrane-spanning domains and that such movements contribute to the diversity of kinetic properties observed for C-type inactivation.
authors.affiliation: Department of Biomedical Engineering, School of Engineering, Duke University, Durham, NC, USA.
authors.forename: R L
authors.initials: RL
authors.lastname: Rasmusson
authors.forename: M J
authors.initials: MJ
authors.lastname: Morales
authors.forename: S
authors.initials: S
authors.lastname: Wang
authors.forename: S
authors.initials: S
authors.lastname: Liu
authors.forename: D L
authors.initials: DL
authors.lastname: Campbell
authors.forename: M V
authors.initials: MV
authors.lastname: Brahmajothi
authors.forename: H C
authors.initials: HC
authors.lastname: Strauss
mesh_terms: D000494:Allosteric Regulation; D000818:Animals; D006321:Heart / Q000502:physiology*; D006801:Humans; D015640:Ion Channel Gating; D008564:Membrane Potentials / Q000502:physiology; D010446:Peptide Fragments / Q000502:physiology; D015221:Potassium Channels / Q000502:physiology*; D011485:Protein Binding
chemical_list: D010446:Peptide Fragments; D015221:Potassium Channels