Isolation of Actinobacillus equuli from the oral cavity of healthy horses and comparison of isolates by restriction enzyme digestion and pulsed-field gel electrophoresis.
Swab samples were collected from the oral cavity of 174 horses in 10 farms and cultured selectively for Actinobacillus equuli. A. equuli could be isolated from 37% of all samples, varying between 12 and 88% in the different farms. Eight horses were sampled repeatedly for several days, with a variation in isolation frequency between 50 and 88%. Isolates were compared by restriction enzyme digestion and Pulsed-Field Gel Electrophoresis. A high degree of strain variability was found within each horse population as well as some variability over time between strains isolated from the same horse.
Sternberg
S
S
Department of Veterinary Microbiology, Faculty of Veterinary Medicine, Swedish University of Agricultural Sciences, Uppsala, Sweden. susanna sternberg@vmm.slu.se
D000188:Actinobacillus / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D000818:Animals; D016000:Cluster Analysis; D015169:Colony Count, Microbial / Q000662:veterinary; D004262:DNA Restriction Enzymes / Q000737:chemistry; D004269:DNA, Bacterial / Q000032:analysis*; D016521:Electrophoresis, Gel, Pulsed-Field / Q000662:veterinary; D005260:Female; D005838:Genotype; D006736:Horses / Q000382:microbiology*; D008297:Male; D009061:Mouth Mucosa / Q000382:microbiology* / Q000503:physiopathology; D010641:Phenotype; D015995:Prevalence; D015183:Restriction Mapping / Q000662:veterinary
D004269:DNA, Bacterial; D004262:DNA Restriction Enzymes