The role of tissue mast cells in polyacrylamide gel-induced inflammation in mice.
OBJECTIVE
In the present study, we investigated the role of mast cells in a model of polyacrylamide gel (PAG)-induced inflammation in mice.


METHODS
Balb/c mice and two strains of mast cell deficient mice (WBB6F1/J-W/Wv, WCB6F1/J-S1/S1d).


METHODS
Various quantities of polyacrylamide gel (Bio-Gel P4) were injected subcutaneously in the backs of mice.


METHODS
Five hours after the injection of PAG the animals were euthanized, the injection sites lavaged and levels of LTB4, PGE2, TNF alpha and cells were determined.


RESULTS
Subcutaneous injection of PAG caused a time-dependent response characterized by the accumulation of inflammatory cells peaking at 10 h and the formation of LTB4, PGE2 and TNF alpha, peaking at 5 h. PAG injection into W/Wv or SL/SLd mice (mice lacking mast cells) resulted in an attenuated response, i.e. LTB4 levels were reduced by 60% and minimal cell influx was seen. The lack of mast cells caused about a 30% reduction in the levels of TNF alpha found.


CONCLUSIONS
These data suggest that mast cells play a prominent role in the PMN influx, TNF alpha production and eicosanoid formation in the PAG-induced inflammatory response.
Harris
R R
RR
Immunoscience Research, Abbott Laboratories, Abbott Park, IL 60064-3500, USA. Richard.R.Harris@abbott.com
Wilcox
D
D
Bell
R L
RL
Carter
G W
GW
D000180:Acrylic Resins; D000818:Animals; D000893:Anti-Inflammatory Agents / Q000494:pharmacology; D015232:Dinoprostone / Q000032:analysis; D006918:Hydroxyurea / Q000031:analogs & derivatives / Q000494:pharmacology; D007249:Inflammation / Q000139:chemically induced* / Q000235:genetics / Q000276:immunology; D007958:Leukocyte Count; D007975:Leukotriene B4 / Q000032:analysis; D016859:Lipoxygenase Inhibitors / Q000494:pharmacology; D008407:Mast Cells / Q000276:immunology*; D051379:Mice; D008807:Mice, Inbred BALB C; D009504:Neutrophils / Q000276:immunology; D009195:Peroxidase / Q000032:analysis; D000099166:Polyacrylamides
D000180:Acrylic Resins; D000893:Anti-Inflammatory Agents; D015232:Dinoprostone; D006918:Hydroxyurea; D007975:Leukotriene B4; D016859:Lipoxygenase Inhibitors; D009195:Peroxidase; D000099166:Polyacrylamides; C082739:A 78773