title: Three-dimensional reconstructions from cryoelectron microscopy images reveal an intimate complex between helicase DnaB and its loading partner DnaC.
abstract: BACKGROUND
  DNA helicases play a fundamental role in all aspects of nucleic acid metabolism and defects in these enzymes have been implicated in a number of inherited human disorders. DnaB is the major replicative DNA helicase in Escherichia coli and has been used as a model system for studying the structure and function of hexameric helicases. The native protein is a hexamer of identical subunits, which in solution forms a complex with six molecules of the loading protein DnaC. DnaB is delivered from this complex onto the DNA template, with the subsequent release of DnaC. We report here the structures of the DnaB helicase hexamer and its complex with DnaC under a defined set of experimental conditions, as determined by three-dimensional cryoelectron microscopy. It was hoped that the structures would provide insight into the mechanisms of helicase activity.
  
  
  RESULTS
  The DnaB structure reveals that six DnaB monomers assemble as three asymmetric dimers to form a polar, ring-like hexamer. The hexamer has two faces, one displaying threefold and the other sixfold symmetry. The six DnaC protomers bind tightly to the sixfold face of the DnaB hexamer. This is the first report of a three-dimensional structure of a helicase obtained using cryoelectron microscopy, and the first report of the structure of a helicase in complex with a loading protein.
  
  
  CONCLUSIONS
  The structures of the DnaB helicase and its complex with DnaC reveal some interesting structural features relevant to helicase function and to the assembly of the two-protein complex. The results presented here provide a basis for a more complete understanding of the structure and function of these important proteins.
authors.affiliation: Centro Nacional de Biotecnología, Universidad Autónoma de Madrid, Cantoblanco, 28049, Madrid, Spain.
authors.forename: C
authors.initials: C
authors.lastname: San Martin
authors.forename: M
authors.initials: M
authors.lastname: Radermacher
authors.forename: B
authors.initials: B
authors.lastname: Wolpensinger
authors.forename: A
authors.initials: A
authors.lastname: Engel
authors.forename: C S
authors.initials: CS
authors.lastname: Miles
authors.forename: N E
authors.initials: NE
authors.lastname: Dixon
authors.forename: J M
authors.initials: JM
authors.lastname: Carazo
mesh_terms: D001426:Bacterial Proteins / Q000737:chemistry* / Q000648:ultrastructure; D004265:DNA Helicases / Q000737:chemistry* / Q000648:ultrastructure; D019281:Dimerization; D053488:DnaB Helicases; D004926:Escherichia coli / Q000201:enzymology*; D029968:Escherichia coli Proteins; D005615:Freezing; D007091:Image Processing, Computer-Assisted; D008854:Microscopy, Electron; D011487:Protein Conformation
chemical_list: D001426:Bacterial Proteins; C071702:DnaC protein, E coli; D029968:Escherichia coli Proteins; D004265:DNA Helicases; D053488:DnaB Helicases