title: The nucleotide and predicted amino acid sequence of the fusion protein of recent isolates of canine distemper virus in Japan.
abstract: Analysis of the molecular properties of fusion (F) proteins of field isolates of canine distemper virus (CDV) by immunoprecipitation analysis revealed an identical molecular mass of F protein of 3 field isolates as well as the Onderstepoort laboratory strain. Sequencing showed that the F gene of a field isolate (the Yanaka strain) shared 90.1% and 95.7% identities with the Onderstepoort strain at nucleotide and amino acid levels, respectively. All of the 13 cysteine residues and 4 potential asparagine-linked glycosylation sites were completely conserved amongst these strains. These results indicate that the F proteins is much less heterogeneous than that observed in the hemagglutinin proteins of CDV.
authors.affiliation: Department of Veterinary Microbiology, Faculty of Agriculture, University of Tokyo, Japan.
authors.forename: K
authors.initials: K
authors.lastname: Iwatsuki
authors.forename: N
authors.initials: N
authors.lastname: Miyashita
authors.forename: E
authors.initials: E
authors.lastname: Yoshida
authors.forename: Y S
authors.initials: YS
authors.lastname: Shin
authors.forename: K
authors.initials: K
authors.lastname: Ohashi
authors.forename: C
authors.initials: C
authors.lastname: Kai
authors.forename: T
authors.initials: T
authors.lastname: Mikami
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D017931:DNA Primers; D004217:Distemper Virus, Canine / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification; D004285:Dogs; D008969:Molecular Sequence Data; D010802:Phylogeny; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid; D012689:Sequence Homology, Nucleic Acid; D014760:Viral Fusion Proteins / Q000096:biosynthesis / Q000737:chemistry* / Q000235:genetics*
chemical_list: D017931:DNA Primers; D014760:Viral Fusion Proteins