title: Capillary gas chromatographic analysis of serum bile acids as the n-butyl ester-trimethylsilyl ether derivatives.
abstract: Gas chromatographic separations of n-butyl ester-trimethylsilyl ether derivatives of several common bile acids were compared with those of the corresponding methyl ester-trimethylsilyl ether derivatives on a CP-Sil-5 CB capillary column. Both types of derivatives were similarly resolved from each other. However, the n-butyl ester-trimethylsilyl ether derivatives of the bile acids showed longer retention times than the corresponding methyl ester-trimethylsilyl ethers and unlike the methyl ester-trimethylsilyl ether derivatives, were completely resolved from and eluted later than the trimethylsilyl ethers of common plasma sterols including sitosterol. A simplified method of plasma work-up for quantitation of bile acids and application of the above method in quantification of plasma bile acids in humans is described.
authors.affiliation: Department of Medicine and the Liver Institute, UMDNJ-NJ Medical School, Newark, NJ, USA.
authors.forename: A K
authors.initials: AK
authors.lastname: Batta
authors.forename: G
authors.initials: G
authors.lastname: Salen
authors.forename: K R
authors.initials: KR
authors.lastname: Rapole
authors.forename: M
authors.initials: M
authors.lastname: Batta
authors.forename: D
authors.initials: D
authors.lastname: Earnest
authors.forename: D
authors.initials: D
authors.lastname: Alberts
mesh_terms: D001647:Bile Acids and Salts / Q000097:blood* / Q000737:chemistry; D002849:Chromatography, Gas / Q000379:methods*; D004952:Esters / Q000737:chemistry; D004987:Ethers / Q000737:chemistry; D006801:Humans; D014297:Trimethylsilyl Compounds / Q000737:chemistry
chemical_list: D001647:Bile Acids and Salts; D004952:Esters; D004987:Ethers; D014297:Trimethylsilyl Compounds