title: Diagnosis of head and neck precancerous lesions in an animal model using fluorescence spectroscopy.
abstract: Laser-induced fluorescence (LIF) of tissues depends on their biochemical and histomorphologic characteristics. LIF spectroscopic properties of 9,10-dimethyl-1,2-benzanthracene (DMBA)-induced precancerous and early cancerous lesions in a hamster buccal pouch mucosa model were studied. Fluorescence spectra from neoplastic lesions showed a characteristic fluorescence peak in the red region of the visible spectrum centered between 630 and 640 nm when excited with 410-nm light. Using this as a diagnostic criterion, 45 of 49 lesions studied were correctly diagnosed, including early dysplastic lesions. Follow-up study of four dysplastic lesions over 2 weeks revealed an increase in red fluorescence intensity. The findings of these experiments suggest that LIF spectroscopy may be a valuable noninvasive technique not only for early diagnosis of head and neck cancer, but also to probe a possible biochemical surrogate biomarker in the follow-up of suspected lesions.
authors.affiliation: Department of Otolaryngology Head and Neck Surgery, New England Medical Center, Tufts University School of Medicine, Boston, Massachusetts 02111, USA.
authors.forename: J K
authors.initials: JK
authors.lastname: Dhingra
authors.forename: X
authors.initials: X
authors.lastname: Zhang
authors.forename: K
authors.initials: K
authors.lastname: McMillan
authors.forename: S
authors.initials: S
authors.lastname: Kabani
authors.forename: R
authors.initials: R
authors.lastname: Manoharan
authors.forename: I
authors.initials: I
authors.lastname: Itzkan
authors.forename: M S
authors.initials: MS
authors.lastname: Feld
authors.forename: S M
authors.initials: SM
authors.lastname: Shapshay
mesh_terms: D015127:9,10-Dimethyl-1,2-benzanthracene / Q000009:adverse effects; D000818:Animals; D014408:Biomarkers, Tumor / Q000032:analysis; D002273:Carcinogens / Q000009:adverse effects; D002278:Carcinoma in Situ / Q000139:chemically induced / Q000175:diagnosis / Q000378:metabolism / Q000473:pathology; D002294:Carcinoma, Squamous Cell / Q000139:chemically induced / Q000175:diagnosis / Q000378:metabolism / Q000473:pathology; D002471:Cell Transformation, Neoplastic / Q000139:chemically induced / Q000378:metabolism / Q000473:pathology; D003116:Color; D006224:Cricetinae; D004195:Disease Models, Animal; D018450:Disease Progression; D005453:Fluorescence; D005500:Follow-Up Studies; D006965:Hyperplasia; D007834:Lasers; D007972:Leukoplakia, Oral / Q000139:chemically induced / Q000175:diagnosis / Q000378:metabolism / Q000473:pathology; D008297:Male; D008647:Mesocricetus; D009061:Mouth Mucosa / Q000187:drug effects / Q000378:metabolism / Q000473:pathology*; D009062:Mouth Neoplasms / Q000139:chemically induced / Q000175:diagnosis* / Q000378:metabolism / Q000473:pathology; D009361:Neoplasm Invasiveness; D011230:Precancerous Conditions / Q000139:chemically induced / Q000175:diagnosis* / Q000378:metabolism / Q000473:pathology; D013050:Spectrometry, Fluorescence; D013280:Stomatitis / Q000139:chemically induced / Q000175:diagnosis / Q000378:metabolism / Q000473:pathology
chemical_list: D014408:Biomarkers, Tumor; D002273:Carcinogens; D015127:9,10-Dimethyl-1,2-benzanthracene