title: Mucosal immunogenicity elicited in mice by oral vaccination with phosphorylcholine encapsulated in poly (D,L-lactide-co-glycolide) microspheres.
abstract: Poly(D,L-lactide-co-glycolide) microspheres loaded with phosphorylcholine linked to thyroglobulin (PC-Thyr) as protein carrier were prepared. The entrapment efficiency reached 80% when the initial protein:polymer ratio was 1:8. Ninety-four percent of the loaded microspheres had a diameter < or = 10 microns. The encapsulation process did not alter PC-Thyr absorbance nor PC antigenic reactivity. Oral administration of these microspheres induced a specific IgA response in intestinal, pulmonary and vaginal secretions, as well as a strong specific systemic immune response in female Balb/c mice. This suggests the need to explore further the potential ability of PC-Thyr loaded microspheres to protect against infections caused by PC-bearing microorganisms which invade or colonize different mucosal sites.
authors.affiliation: Service de Microbiologie Médicale, Institut Gustave-Roussy, Villejuif, France.
authors.forename: K
authors.initials: K
authors.lastname: Allaoui-Attarki
authors.forename: E
authors.initials: E
authors.lastname: Fattal
authors.forename: S
authors.initials: S
authors.lastname: Pecquet
authors.forename: S
authors.initials: S
authors.lastname: Trollé
authors.forename: E
authors.initials: E
authors.lastname: Chachaty
authors.forename: P
authors.initials: P
authors.lastname: Couvreur
authors.forename: A
authors.initials: A
authors.lastname: Andremont
mesh_terms: D000284:Administration, Oral; D000818:Animals; D001672:Biocompatible Materials / Q000008:administration & dosage; D016503:Drug Delivery Systems; D005260:Female; D018928:Immunity, Mucosal / Q000187:drug effects* / Q000276:immunology*; D019344:Lactic Acid / Q000008:administration & dosage*; D051379:Mice; D008807:Mice, Inbred BALB C; D008863:Microspheres; D010767:Phosphorylcholine / Q000008:administration & dosage* / Q000276:immunology*; D011100:Polyglycolic Acid / Q000008:administration & dosage*; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011108:Polymers / Q000008:administration & dosage*; D013954:Thyroglobulin / Q000008:administration & dosage / Q000276:immunology; D014612:Vaccines / Q000008:administration & dosage*
chemical_list: D001672:Biocompatible Materials; D011108:Polymers; D014612:Vaccines; D010767:Phosphorylcholine; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011100:Polyglycolic Acid; D019344:Lactic Acid; D013954:Thyroglobulin