Double (fluorescent and spin) sensors for detection of reactive oxygen species in the thylakoid membrane.
A series of dansylated sterically hindered amines designed to trapping reactive oxygen species, were synthesized. Compounds were tested in isolated thylakoid membranes subjected to photoinhibition by excess photosynthetically active radiation (400-700 nm). DanePy showed good selectivity for singlet oxygen and the formation of nitroxide was detected by appearance of ESR signal and quenching fluorescence.
Kálai
T
T
Institute of Organic and Medicinal Chemistry, University of Pécs, Hungary.
Hideg
E
E
Vass
I
I
Hideg
K
K
D002736:Chloroplasts / Q000737:chemistry* / Q000648:ultrastructure; D003497:Cyclic N-Oxides / Q000737:chemistry; D003619:Dansyl Compounds / Q000138:chemical synthesis*; D004578:Electron Spin Resonance Spectroscopy; D007425:Intracellular Membranes / Q000737:chemistry; D008027:Light; D009589:Nitrogen Oxides / Q000378:metabolism; D010100:Oxygen / Q000032:analysis*; D010880:Piperidines / Q000138:chemical synthesis*; D017382:Reactive Oxygen Species / Q000378:metabolism*; D026082:Singlet Oxygen; D013050:Spectrometry, Fluorescence; D013113:Spin Labels; D018724:Spinacia oleracea
D003497:Cyclic N-Oxides; C112332:DanePy; D003619:Dansyl Compounds; D009589:Nitrogen Oxides; D010880:Piperidines; D017382:Reactive Oxygen Species; D013113:Spin Labels; D026082:Singlet Oxygen; C039900:nitroxyl; D010100:Oxygen; C003959:TEMPO