title: LOCATE enzyme-linked immunosorbent assay for detection of Salmonella in food: collaborative study.
abstract: A collaborative study was performed in 27 laboratories to validate the enzyme-linked immunosorbent procedure LOCATE for rapid detection of Salmonella in foods. Results were read visually and with a microtiter plate reader. The LOCATE method was compared with the Bacteriological Analytical Manual (BAM)/AOAC INTERNATIONAL culture method for detecting Salmonella in 6 foods: milk chocolate, nonfat dry milk, dried whole egg, soy flour, ground black pepper, and ground raw turkey. Two foods--dried whole egg and black pepper--required repeat rounds because insufficient data sets were produced initially (AOAC INTERNATIONAL stipulates a minimum of 15 sets per food type). Each laboratory tested one or more of the 6 foods. A total of 1 439 samples were analyzed, and no significant differences (P < 0.05) were observed between LOCATE with either visual or reader detection and BAM/AOAC INTERNATIONAL results. The LOCATE screening method with visual or reader detection is recommended for Official First Action Approval.
authors.affiliation: Silliker Laboratories Group, Inc., Corporate Research Center, South Holland, IL 60473, USA.
authors.forename: V
authors.initials: V
authors.lastname: Gangar
authors.forename: M S
authors.initials: MS
authors.lastname: Curiale
authors.forename: A
authors.initials: A
authors.lastname: D'Onorio
authors.forename: C
authors.initials: C
authors.lastname: Donnelly
authors.forename: P
authors.initials: P
authors.lastname: Dunnigan
mesh_terms: D000818:Animals; D002099:Cacao / Q000737:chemistry / Q000382:microbiology; D004531:Eggs / Q000032:analysis / Q000382:microbiology; D004797:Enzyme-Linked Immunosorbent Assay; D005433:Flour / Q000032:analysis / Q000382:microbiology; D005506:Food Contamination / Q000032:analysis*; D005516:Food Microbiology; D007202:Indicators and Reagents; D008460:Meat / Q000032:analysis / Q000382:microbiology; D008892:Milk / Q000737:chemistry / Q000382:microbiology; D015203:Reproducibility of Results; D012475:Salmonella / Q000737:chemistry*; D012703:Serotyping; D017365:Spices / Q000032:analysis / Q000382:microbiology; D014422:Turkeys
chemical_list: D007202:Indicators and Reagents