Sodium alkyl ether sulfate preparative electrophoresis for the preparation of reaction centers without H-subunit from Rhodopseudomonas viridis.
Sodium alkyl ether sulfate (AES), an analog of sodium dodecyl sulfate (SDS) was used in polyacrylamide gel electrophoresis (PAGE) for the partial decomposition of the photosynthetic reaction center (RC) of Rhodopseudomonas viridis. Unlike SDS, AES did not completely dissociate RC into its subunits but selectively detached H-subunit from RC to give RC(-H) without losing the spectroscopic nature of RC. For the denaturation of RC(-H), AES was found to be as mild as 3-[3-cholamidopropyl)dimethylammonio]-l-propanesulfonate (CHAPS).
Miyake
J
J
National Institute for Advanced Interdisciplinary Research, Tsukuba, Ibaraki, Japan. miyake@nair.go.jp
Hara
M
M
Asada
Y
Y
Morimoto
Y
Y
Shirai
M
M
D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods*; D045322:Photosynthetic Reaction Center Complex Proteins / Q000032:analysis*; D012241:Rhodopseudomonas / Q000737:chemistry*; D012967:Sodium Dodecyl Sulfate / Q000031:analogs & derivatives*; D013501:Surface-Active Agents
D045322:Photosynthetic Reaction Center Complex Proteins; D013501:Surface-Active Agents; D012967:Sodium Dodecyl Sulfate