Chemical imaging of nucleic acids, proteins and lipids of a single living cell. Application of synchrotron infrared microspectrometry in cell biology.
Hybridoma B-cells have been used as models to evaluate the performance of synchrotron infrared microscopy to obtain chemical images of a single living cell. Chemical mapping of nucleic acids, proteins and lipids at a resolution of a few microns, close to the diffraction limit in the mid-infrared region are shown.
Jamin
N
N
CEA-INSTN-CIE, Gif-Sur-Yvette, France.
Dumas
P
P
Moncuit
J
J
Fridman
W H
WH
Teillaud
J L
JL
Carr
G L
GL
Williams
G P
GP
D000818:Animals; D008055:Lipids / Q000032:analysis*; D051379:Mice; D009696:Nucleic Acids / Q000032:analysis*; D011506:Proteins / Q000032:analysis*; D013055:Spectrophotometry, Infrared / Q000379:methods*; D017356:Synchrotrons; D014407:Tumor Cells, Cultured
D008055:Lipids; D009696:Nucleic Acids; D011506:Proteins