The Bacillus subtilis galE gene is essential in the presence of glucose and galactose.
Bacillus subtilis is unable to grow by consuming galactose because it is unable to transport it into the cell. The transcription of galE is not influenced by galactose but is repressed by glucose. Galactose is toxic for galE-negative bacteria because it results in elevated levels of metabolic intermediates. These negative effects are reduced in galK and galT mutants. Glucose is also toxic for galE-negative strains.
Krispin
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O
Lehrstuhl für Mikrobiologie, Universität Erlangen-Nürnberg, Erlangen, Germany.
Allmansberger
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R
D001412:Bacillus subtilis / Q000235:genetics / Q000254:growth & development* / Q000378:metabolism; D001426:Bacterial Proteins / Q000096:biosynthesis / Q000235:genetics; D001483:Base Sequence; D017931:DNA Primers; D004794:Enzyme Repression; D004926:Escherichia coli / Q000235:genetics; D005690:Galactose / Q000378:metabolism / Q000494:pharmacology*; D015964:Gene Expression Regulation, Bacterial / Q000187:drug effects*; D005838:Genotype; D005947:Glucose / Q000378:metabolism / Q000494:pharmacology*; D007700:Kinetics; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D011401:Promoter Regions, Genetic; D014158:Transcription, Genetic; D014534:UDPglucose 4-Epimerase / Q000096:biosynthesis* / Q000235:genetics*
D001426:Bacterial Proteins; D017931:DNA Primers; D014534:UDPglucose 4-Epimerase; C020551:galactose epimerase; D005947:Glucose; D005690:Galactose