title: The DRPLA CAG repeats in an Italian population sample: evaluation of the polymorphism for forensic applications.
abstract: The DRPLA CAG repeats polymorphism has been studied in an Italian population sample. PCR amplification, manual PAGE and silver staining were employed. A total of 16 different alleles, spanning the range from 5 to 21 CAG triplettes, was observed. The heterozygosity was 0.81 and no significant deviation from Hardy-Weinberg equilibrium was found 81 meioses from parentage testing were also analyzed and a Mendelian pattern of inheritance was observed in all cases. In addition, we could successfully type DRPLA locus in some forensic specimens, 1 ng of DNA allowing clear definition of alleles. The authors conclude that the DRPLA CAG repeats analysis may be useful for forensic applications.
authors.affiliation: Department of Medicine and Public Health, University of Bologna, Italy.
authors.forename: S
authors.initials: S
authors.lastname: Pelotti
authors.forename: V
authors.initials: V
authors.lastname: Mantovani
authors.forename: P D
authors.initials: PD
authors.lastname: Esposti
authors.forename: L
authors.initials: L
authors.lastname: D'Apote
authors.forename: M
authors.initials: M
authors.lastname: Bragliani
authors.forename: E
authors.initials: E
authors.lastname: Maiolini
authors.forename: A
authors.initials: A
authors.lastname: Abbondanza
authors.forename: G
authors.initials: G
authors.lastname: Pappalardo
mesh_terms: D000483:Alleles; D004247:DNA / Q000032:analysis*; D016172:DNA Fingerprinting / Q000379:methods; D017931:DNA Primers / Q000737:chemistry; D005260:Female; D006801:Humans; D007558:Italy; D008297:Male; D009419:Nerve Tissue Proteins / Q000235:genetics*; D019636:Neurodegenerative Diseases / Q000235:genetics*; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic; D018911:Trinucleotide Repeats / Q000235:genetics*
chemical_list: D017931:DNA Primers; D009419:Nerve Tissue Proteins; C095278:atrophin-1; D004247:DNA