title: Identification of conserved potentially regulatory sequences of the SRY gene from 10 different species of mammals.
abstract: We have sequenced the 5' region of the SRY gene from human, chimpanzee, sheep, and mouse and from four additional mammalian species, not previously characterized (gorilla, gazelle, rat, and guinea pig). In order to identify conserved DNA elements potentially involved in the regulation of the SRY gene, the newly determined sequences were analyzed and compared to all mammalian SRY promoter sequences available at present. Ten highly conserved potential regulatory elements have been identified in all 10 species (AP1, Barbie, GATA, Gfi1, cMyb, vMyb, NF1, Oct1, Sp1, and SRY). The known function of several of these regulatory elements fits well with the known expression of the SRY gene. However, except for the highly conserved coding HMG motif, only a short region close to the initiation of transcription in the human SRY is conserved in the exact position along the gene in all the species analyzed. This lack of sequence identity at the orthologous positions is consistent with the suggested rapid evolution of the SRY gene. This relative lack of homology contrasts with a high sequence identity of the putative regulatory sequences found within each taxonomic group of species (primates, bovids, and rodents), which supports a common mechanism of SRY expression and possibly also a similar function.
authors.affiliation: Hospital Clínic, Institut de Investigacions Biomèdiques August Pi i Sunyer (IDIBAPS), Barcelona, Spain.
authors.forename: E
authors.initials: E
authors.lastname: Margarit
authors.forename: A
authors.initials: A
authors.lastname: Guillén
authors.forename: C
authors.initials: C
authors.lastname: Rebordosa
authors.forename: J
authors.initials: J
authors.lastname: Vidal-Taboada
authors.forename: M
authors.initials: M
authors.lastname: Sánchez
authors.forename: F
authors.initials: F
authors.lastname: Ballesta
authors.forename: R
authors.initials: R
authors.lastname: Oliva
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D001665:Binding Sites / Q000235:genetics; D003001:Cloning, Molecular; D017124:Conserved Sequence / Q000235:genetics*; D004268:DNA-Binding Proteins / Q000737:chemistry* / Q000502:physiology; D019143:Evolution, Molecular; D008322:Mammals; D008969:Molecular Sequence Data; D009687:Nuclear Proteins; D010802:Phylogeny; D011401:Promoter Regions, Genetic / Q000235:genetics*; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D051876:Sex-Determining Region Y Protein; D014157:Transcription Factors
chemical_list: D004268:DNA-Binding Proteins; D009687:Nuclear Proteins; D051876:Sex-Determining Region Y Protein; D014157:Transcription Factors