Regulation of Cdc2 activity by phosphorylation at T14/Y15.
The highly conserved Cdc2 serine/threonine kinase plays a central role in cell cycle progression. Although Cdc2 levels remain constant throughout the cell cycle, Cdc2 kinase activity peaks at the G2/M boundary, in order to drive entry into mitosis. In the model organism Schizosaccharomysces pombe, potentially active Cdc2/Cdc13 kinase complex accumulates throughout the S and G2 phases of the cell cycle. This complex, however, is maintained in an active state by Wee1/Mik1-mediated phosphorylation at Y15 (and, possibly, T14). At the G2/M boundary, the Cdc25 protein phosphatase is activated to dephosphorylate the Cdc2/Cdc13 complex, resulting in abrupt activation of Cdc2 kinase activity and entry into mitosis.
Berry
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Howard Hughes Medical Institute, Department of Cell Biology, Vanderbilt University, Nashville, TN 37212, USA.
Gould
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KL
D000818:Animals; D001665:Binding Sites; D016203:CDC2 Protein Kinase / Q000737:chemistry / Q000378:metabolism*; D002453:Cell Cycle / Q000502:physiology; D018797:Cell Cycle Proteins; D018844:Cyclin-Dependent Kinases / Q000378:metabolism; D004789:Enzyme Activation; D006801:Humans; D009687:Nuclear Proteins; D010766:Phosphorylation; D011505:Protein-Tyrosine Kinases / Q000378:metabolism; D012568:Schizosaccharomyces / Q000166:cytology / Q000201:enzymology / Q000235:genetics; D029702:Schizosaccharomyces pombe Proteins
D018797:Cell Cycle Proteins; D009687:Nuclear Proteins; D029702:Schizosaccharomyces pombe Proteins; C068689:wee1 protein, S pombe; D011505:Protein-Tyrosine Kinases; C499349:WEE1 protein, human; D016203:CDC2 Protein Kinase; D018844:Cyclin-Dependent Kinases