Comparison of the protein patterns of Erysipelothrix rhusiopathiae strains by SDS-PAGE and autoradiography.
The proteins of 12 Erysipelothrix rhusiopathiae strains were radiolabelled with L-[35S] methionine, and the protein fractions were detected by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and autoradiography. The strains were found to differ from each other in their protein patterns; thus, it was concluded that the method applied could provide useful data for the identification of strains. The described procedure was used for determining the percentage contents of protein fractions of identical molecular mass within the strains compared. The results show that there is no significant correlation between the type of the strains and the percentage of identical molecular weight fractions.
Bernáth
S
S
Hungarian State Institute for the Control of Veterinary Biologicals, Drugs and Feeds, Budapest, Hungary.
Kucsera
G
G
Kádár
I
I
Horváth
G
G
Morovján
G
G
D000818:Animals; D001345:Autoradiography; D001426:Bacterial Proteins / Q000032:analysis* / Q000737:chemistry / Q000145:classification; D003720:Densitometry; D004591:Electrophoresis, Polyacrylamide Gel; D004888:Erysipelothrix / Q000737:chemistry / Q000145:classification*; D008970:Molecular Weight; D015203:Reproducibility of Results; D012703:Serotyping
D001426:Bacterial Proteins