title: Sporulation and time course expression of sigma-factor homologous genes in Clostridium acetobutylicum.
abstract: The gene for the vegetative sigma factor A of Clostridium acetobutylicum was constitutively transcribed during growth and formed an operon together with dnaE. Sporulation-specific sigma factors E, G, and K were sequentially induced shortly before mature endospores could be detected. Maximal transcription in the course of spore formation was found to be in the order sigE-sigG-sigK, thus matching the pattern described for Bacillus subtilis. From primer extension experiments promoter structures could be deduced with high homology to the Bacillus consensus. Upstream of the spoIIGA-sigE operon a gene with significant similarity to ftsZ could be detected.
authors.affiliation: Institut für Mikrobiologie, Georg-August-Universität, Göttingen, Germany.
authors.forename: J D
authors.initials: JD
authors.lastname: Santangelo
authors.forename: A
authors.initials: A
authors.lastname: Kuhn
authors.forename: A
authors.initials: A
authors.lastname: Treuner-Lange
authors.forename: P
authors.initials: P
authors.lastname: Dürre
mesh_terms: D003013:Clostridium / Q000235:genetics* / Q000502:physiology; D005798:Genes, Bacterial; D010710:Phosphates / Q000378:metabolism; D011401:Promoter Regions, Genetic; D012333:RNA, Messenger / Q000032:analysis; D012808:Sigma Factor / Q000235:genetics*; D013171:Spores, Bacterial / Q000502:physiology
chemical_list: D010710:Phosphates; D012333:RNA, Messenger; D012808:Sigma Factor