title: The effect of high altitude and caloric restriction on reactive carbonyl derivatives and activity of glutamine synthetase in rat brain.
abstract: Both exposure to high altitude (HA) and caloric restriction (CR) may influence free radical generation. The aim of the present study was to evaluate the effects of 4 wk chronic exposure to 4,000m of HA and CR (60% of CR of control (C) rats) on protein oxidation in brain. Eighteen rats with similar body mass were assigned to CR, HA and C rats. Reactive carbonyl derivatives (RCD), marker of protein oxidation, were measured by Western blot. In addition, the activity and protein content of glutamine synthetase (GS) were determined. The body mass of C rats was significantly higher (P< 0.001) than that of HA and CR groups. The quantified signal intensity of RCD was significantly stronger in C rats than in HA and CR rats. The activity of GS was significantly increased in CR rats, while the protein content of GS was decreased in HA rats compared to C group. The data suggest that both HA and CR decreases the accumulation of RCD in the brain, however the mechanism of the decrease seems to be different during HA and CR.
authors.affiliation: Laboratory of Exercise Physiology, Institute of Sport and Health Sciences, University of Tsukuba, Japan. radak@mte.hupe.hu
authors.forename: Z
authors.initials: Z
authors.lastname: Radák
authors.forename: K
authors.initials: K
authors.lastname: Asano
authors.forename: Y
authors.initials: Y
authors.lastname: Fu
authors.forename: A
authors.initials: A
authors.lastname: Nakamura
authors.forename: H
authors.initials: H
authors.lastname: Nakamoto
authors.forename: H
authors.initials: H
authors.lastname: Ohno
authors.forename: S
authors.initials: S
authors.lastname: Goto
mesh_terms: D000531:Altitude; D000818:Animals; D001274:Atmospheric Pressure; D001835:Body Weight / Q000502:physiology; D001921:Brain / Q000201:enzymology* / Q000378:metabolism; D002149:Energy Intake; D005609:Free Radicals / Q000378:metabolism; D005974:Glutamate-Ammonia Ligase / Q000378:metabolism*; D006835:Hydrazones / Q000378:metabolism*; D008297:Male; D009419:Nerve Tissue Proteins / Q000096:biosynthesis; D010084:Oxidation-Reduction; D018384:Oxidative Stress / Q000502:physiology; D051381:Rats; D017208:Rats, Wistar
chemical_list: D005609:Free Radicals; D006835:Hydrazones; D009419:Nerve Tissue Proteins; D005974:Glutamate-Ammonia Ligase