Molecular cloning of a cDNA encoding molt-inhibiting hormone of the crab, Cancer magister.
A neuropeptide molt-inhibiting hormone (MIH) negatively regulates crustacean molting glands (Y-organs). We report here the molecular cloning of a cDNA encoding putative MIH of the Dungeness crab, Cancer magister. A cDNA library was commercially prepared using poly (A+) RNA isolated from C. magister eyestalk neural ganglia. The library was screened using as probe a previously cloned portion of a cDNA encoding MIH of the blue crab, Callinectes sapidus. DNA sequence analysis of one positive clone revealed a 339 base pair open reading frame encoding a 78 amino acid putative MIH and a 35 amino acid signal peptide. The deduced amino acid sequence of C. magister MIH shows high sequence identity (80-98%) with MIH of three other brachyuran crabs, but lower identity (26-45%) with MIH and MIH-like peptides from astacurans and shrimp. Studies using reverse transcription-polymerase chain reaction (RT-PCR) indicate the MIH gene is expressed in eyestalk but not control (muscle, gill, gonad, hepatopancreas) tissue.
Umphrey
H R
HR
Department of Biology, University of Alabama at Birmingham, 35294, USA.
Lee
K J
KJ
Watson
R D
RD
Spaziani
E
E
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003386:Brachyura / Q000235:genetics*; D003001:Cloning, Molecular; D016384:Consensus Sequence; D018076:DNA, Complementary / Q000737:chemistry / Q000235:genetics*; D015723:Gene Library; D007447:Invertebrate Hormones / Q000235:genetics*; D008969:Molecular Sequence Data; D021382:Protein Sorting Signals / Q000737:chemistry; D017385:Sequence Homology
D018076:DNA, Complementary; D007447:Invertebrate Hormones; D021382:Protein Sorting Signals; C012445:molt-inhibiting hormone