Studies on the interaction between hyaluronan and a rat colon cancer cell line.
Binding studies with 125I-Tyr labelled hyaluronan (HA) on a cultured rat colon cancer cell line were performed to characterize the association of HA to tumour cells in vitro. Results show a specific and saturable binding (Kd=1.36 nM) which indicates the presence of an HA binding receptor on the tumour cells. There is a specific constant increase of cell-associated HA over time, which indicates that HA is specifically taken up by the cells through endocytosis. The binding of 125I-Tyr labelled HA was more effectively inhibited by unlabelled HA of high MW in relation to low MW species of the polysaccharide indicating that the receptor binds HA of high MW with greater affinity than low MW species. In competition experiments, the HA-binding could not be inhibited by other polysaccharides such as chondroitin sulphate and heparin. Nor could ligands for scavenger receptors and antibodies directed towards ICAM-1, CD 44 and RHAMM (Receptor for HA Mediated Motility) significantly inhibit the association of HA to tumour cells.
Samuelsson
C
C
Department of Medical and Physiological Chemistry, University of Uppsala, Sweden.
Gustafson
S
S
D000818:Animals; D000906:Antibodies / Q000494:pharmacology; D002809:Chondroitin Sulfates / Q000494:pharmacology; D003110:Colonic Neoplasms / Q000188:drug therapy / Q000378:metabolism*; D016264:Dextran Sulfate / Q000494:pharmacology; D004727:Endothelium / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D016326:Extracellular Matrix Proteins / Q000276:immunology / Q000378:metabolism; D018960:Hyaluronan Receptors / Q000276:immunology / Q000378:metabolism; D006820:Hyaluronic Acid / Q000378:metabolism* / Q000493:pharmacokinetics; D018799:Intercellular Adhesion Molecule-1 / Q000276:immunology / Q000378:metabolism; D008099:Liver / Q000166:cytology; D051379:Mice; D051381:Rats; D017208:Rats, Wistar; D014407:Tumor Cells, Cultured
D000906:Antibodies; D016326:Extracellular Matrix Proteins; D018960:Hyaluronan Receptors; C098946:hyaluronan-mediated motility receptor; D018799:Intercellular Adhesion Molecule-1; D006820:Hyaluronic Acid; D002809:Chondroitin Sulfates; D016264:Dextran Sulfate