Determination of allantoin in bovine milk by high-performance liquid chromatography.
Determination of allantoin in bovine milk based on high-performance liquid chromatography (HPLC) is described. Following dilution and filtration, milk samples were analysed directly. Separation and quantification of allantoin was achieved using a Spherisorb 5 NH2 column (250 x 4.6 mm ID), acetonitrile-water (90:10, v/v) mobile phase at a flow-rate of 2.0 ml min(-1), temperature 20 degrees C and monitoring the effluent at 214 nm. Total analysis time was 10 min. Recovery of allantoin added to milk was 97 (+/-3.7, n = 30)%. Lowest detectable concentration was 1 micromol l(-1). Within-day and between-day variability were less than 3%. Advantages of improved retention and separation of allantoin, and less complicated sample preparation exist over current methods.
Shingfield
K J
KJ
Department of Grassland and Ruminant Science, SAC, Ayr, Scotland, UK.
Offer
N W
NW
D000481:Allantoin / Q000032:analysis*; D000818:Animals; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D002940:Circadian Rhythm; D008892:Milk / Q000737:chemistry*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013056:Spectrophotometry, Ultraviolet
D000481:Allantoin