Stimulation of hemopoietic colony formation from mouse marrow cells in vitro using human dialyzable leukocyte extracts-IMMODIN-SEVAC.
The influence of dialyzable extract from human leukocytes (DLE) on the in vitro growth of the granulocyte-macrophage colony-forming cell (GM-CFC) colonies from progenitors of mouse bone marrow cells was studied. DLE alone did not induce the colony growth but it modulated the number of colonies if administered together with a colony-stimulating factor (CSF). The costimulatory effect prevailed in a broad range of DLE dilution and the index of increase was enhanced with the lowering of the CSF concentration. The costimulatory augmentation of clonal proliferation of GM-CFC with DLE was further strengthened by addition of indomethacin, thus indicating an intervening role of prostaglandins in the modulatory influence of DLE.
Vacek
A
A
SEVAC a.s. Prague, Czećh Republic.
Barnet
K
K
Hofer
M
M
Cech
K
K
Pekárek
J
J
Schneiderová
H
H
D000818:Animals; D001854:Bone Marrow Cells / Q000166:cytology* / Q000187:drug effects; D002457:Cell Extracts / Q000494:pharmacology*; D017077:Culture Media, Conditioned; D016207:Cytokines / Q000494:pharmacology*; D003956:Dialysis; D016178:Granulocyte-Macrophage Colony-Stimulating Factor / Q000494:pharmacology / Q000502:physiology; D006412:Hematopoietic Stem Cells / Q000166:cytology* / Q000187:drug effects; D006801:Humans; D007962:Leukocytes / Q000187:drug effects / Q000502:physiology*; D051379:Mice; D008810:Mice, Inbred C57BL; D008808:Mice, Inbred CBA; D013268:Stimulation, Chemical
D002457:Cell Extracts; D017077:Culture Media, Conditioned; D016207:Cytokines; D016178:Granulocyte-Macrophage Colony-Stimulating Factor