Cyclosporine A modulation of Ca++ activated K+ channels in cardiac sensory afferent neurons.
Whole-cell and single channel recordings were used to characterize the effects of the immunosuppressant cyclosporine A (CsA) on cardiac sensory neurons (CSN) of the nodose ganglia. Application of 10 nM CsA resulted in a 29.1% decrease in CSN input resistance and an average -8+/-3 mV hyperpolarization of membrane potential. Application of 10 nM CsA had no effect on evoked Ca++ currents but increased evoked K+ currents by 158.9+/-24%. Application of 10 nM CsA significantly increased the open probability of KCa channels by 183+/-9%. These results suggest that application of CsA results in the activation of KCa channels in cardiac sensory neurons and this effect may contribute to the cellular mechanisms underlying CsA modulation of vagal afferent neurons.
Hay
M
M
Department of Veterinary Biomedical Sciences, Dalton Cardiovascular Research Center, University of Missouri, Columbia, MO 65211, USA. dcrcmh@showme.missouri.edu
D000818:Animals; D002118:Calcium / Q000502:physiology*; D015220:Calcium Channels / Q000378:metabolism; D016572:Cyclosporine / Q000494:pharmacology*; D004594:Electrophysiology; D006329:Heart Conduction System / Q000166:cytology / Q000378:metabolism*; D007166:Immunosuppressive Agents / Q000494:pharmacology*; D015640:Ion Channel Gating / Q000502:physiology; D009475:Neurons, Afferent / Q000378:metabolism*; D018408:Patch-Clamp Techniques; D015221:Potassium Channels / Q000187:drug effects / Q000378:metabolism*; D051381:Rats
D015220:Calcium Channels; D007166:Immunosuppressive Agents; D015221:Potassium Channels; D016572:Cyclosporine; D002118:Calcium