title: Expression of human estrogen sulfotransferase in Salmonella typhimurium: differences between hHST and hEST in the enantioselective activation of 1-hydroxyethylpyrene to a mutagen.
abstract: Various human sulfotransferases (hP-PST, hM-PST, hHST) and rat sulfotransferases (rPST-IV, rHSTa) have already been expressed in Ames' Salmonella strains (in particular in TA1538). Now a further strain, TA1538-hEST, which expresses the human estrogen sulfotransferase (hEST), has been constructed. This strain activated the primary benzylic alcohol 1-hydroxymethylpyrene (1-HMP) and the secondary benzylic alcohol 1-hydroxyethylpyrene (1-HEP) to mutagens. Human sulfotransferases hEST and hHST both activated 1-HEP, but they differed substantially in their enantioselectivity for this compound.
authors.affiliation: German Institute of Human Nutrition, Potsdam-Rehbrücke.
authors.forename: M
authors.initials: M
authors.lastname: Hagen
authors.forename: U
authors.initials: U
authors.lastname: Pabel
authors.forename: R
authors.initials: R
authors.lastname: Landsiedel
authors.forename: I
authors.initials: I
authors.lastname: Bartsch
authors.forename: C N
authors.initials: CN
authors.lastname: Falany
authors.forename: H
authors.initials: H
authors.lastname: Glatt
mesh_terms: D000818:Animals; D001592:Benzyl Alcohols / Q000493:pharmacokinetics / Q000633:toxicity; D015153:Blotting, Western; D003600:Cytosol / Q000201:enzymology; D006801:Humans; D009152:Mutagenicity Tests; D009153:Mutagens / Q000493:pharmacokinetics* / Q000633:toxicity; D051381:Rats; D012486:Salmonella typhimurium / Q000187:drug effects / Q000201:enzymology* / Q000235:genetics; D013237:Stereoisomerism; D013379:Substrate Specificity; D015238:Sulfotransferases / Q000096:biosynthesis / Q000235:genetics / Q000378:metabolism*
chemical_list: D001592:Benzyl Alcohols; D009153:Mutagens; D015238:Sulfotransferases; C028152:alcohol sulfotransferase; C020122:estrone sulfotransferase