title: Studies of the binding and structure of adrenocorticotropin peptides in membrane mimics by NMR spectroscopy and pulsed-field gradient diffusion.
abstract: The partition and structure of three adrenocorticotropic hormone peptides ACTH(1-10), ACTH(1-24), and ACTH(11-24) in water and in sodium dodecylsulfate (SDS) and dodecylphosphocholine (DPC) micelles were studied by 2D NMR and NMR gradient diffusion measurements. The diffusion rates, the NH chemical shifts, and the nuclear Overhauser effect patterns provided a coherent picture of binding of these peptides. All three peptides are significantly partitioned in the negatively charged SDS micelles and possess definite secondary structure, as opposed to random structures in water. For ACTH (1-24), the hydrophobic 1-10 segment is partitioned in DPC micelles, but the charged 11-24 segment prefers to remain in the aqueous region. ACTH(11-24) does not bind significantly to the DPC micelles. The binding of the ACTH peptides in these two widely used "membrane mimics" are substantially different from that in 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine (POPC) bilayers obtained by attenuated total reflection infrared spectroscopy and from our preliminary diffusion studies of the same peptides in POPC vesicles. This study showed that, in a given micellar medium, all corresponding segments of these peptides are located in the same membrane environment in the system, regardless of whether these segments exist by themselves or are attached to other segments. This result may contradict the membrane-compartments concept of Schwyzer, which suggests that ACTH(1-10) and ACTH(1-24) are located in different membrane compartments because they have different address segments, and consequently, bind to different receptors. The present results also suggest that the assumption that micelles are good membrane mimics should be carefully examined.
authors.affiliation: Department of Chemistry, University of Missouri, Columbia 65211, USA.
authors.forename: X
authors.initials: X
authors.lastname: Gao
authors.forename: T C
authors.initials: TC
authors.lastname: Wong
mesh_terms: D000324:Adrenocorticotropic Hormone / Q000737:chemistry* / Q000378:metabolism*; D055592:Biophysical Phenomena; D001703:Biophysics; D003366:Cosyntropin / Q000737:chemistry / Q000378:metabolism; D004058:Diffusion; D066298:In Vitro Techniques; D008567:Membranes, Artificial; D008823:Micelles; D019906:Nuclear Magnetic Resonance, Biomolecular; D010446:Peptide Fragments / Q000737:chemistry* / Q000378:metabolism*; D010767:Phosphorylcholine / Q000031:analogs & derivatives; D011392:Proline / Q000737:chemistry; D011485:Protein Binding; D017433:Protein Structure, Secondary; D012967:Sodium Dodecyl Sulfate; D013237:Stereoisomerism
chemical_list: D008567:Membranes, Artificial; D008823:Micelles; D010446:Peptide Fragments; D010767:Phosphorylcholine; D003366:Cosyntropin; D012967:Sodium Dodecyl Sulfate; C011393:ACTH (1-10); C034016:ACTH (11-24); C028810:dodecylphosphocholine; D000324:Adrenocorticotropic Hormone; D011392:Proline