title: Sequestration of the G protein beta gamma subunit complex inhibits receptor-mediated endocytosis.
abstract: Cell surface receptors that mediate endocytosis cluster into clathrin-coated pits, which pinch off to form vesicles that transport the receptors and their ligands. This multi-step process requires the coordinated action of many factors, including GTP-hydrolyzing proteins such as dynamin and regulators of actin cytoskeleton assembly. We note herein that sequestration of heterotrimeric G protein beta gamma subunits in intact cells strongly inhibits clathrin-coated pit-mediated endocytosis and causes rearrangement of the actin cytoskeleton. Our results suggest that cells contain a pool of free beta gamma and that it functions constitutively to permit endocytosis.
authors.affiliation: Department of Pharmacology, University of Texas Southwestern Medical Center, Dallas, TX 75235-9041, USA.
authors.forename: H C
authors.initials: HC
authors.lastname: Lin
authors.forename: J A
authors.initials: JA
authors.lastname: Duncan
authors.forename: T
authors.initials: T
authors.lastname: Kozasa
authors.forename: A G
authors.initials: AG
authors.lastname: Gilman
mesh_terms: D008841:Actin Cytoskeleton / Q000648:ultrastructure; D000199:Actins / Q000502:physiology; D000256:Adenoviridae / Q000235:genetics; D000818:Animals; D019556:COS Cells; D003034:Coated Pits, Cell-Membrane / Q000502:physiology; D004705:Endocytosis; D019206:GTP-Binding Protein alpha Subunits, Gi-Go / Q000502:physiology*; D005822:Genetic Vectors; D011994:Recombinant Proteins; D014168:Transferrin / Q000378:metabolism
chemical_list: D000199:Actins; D011994:Recombinant Proteins; D014168:Transferrin; D019206:GTP-Binding Protein alpha Subunits, Gi-Go