Projection map of the reaction center-light harvesting 1 complex from Rhodopseudomonas viridis at 10 A resolution.
The photosynthetic reaction center-light harvesting 1 complex from Rhodopseudomonas viridis was purified and reconstituted into two-dimensional crystals. The single-layered crystalline sheets with lattice parameters a=b=133.3 A and gamma=120 degrees were investigated by electron cryo-microscopy and the projection map at 10 A resolution was calculated. The opening diameter of the light-harvesting ring of 72 A is sufficient to allow slight movement of the reaction center within the ring. Based on characteristic features observed in the projection map, the mechanism of energy transfer from the light-harvesting 1 complex to the reaction center was discussed.
Ikeda-Yamasaki
I
I
Faculty of Engineering Science, Osaka University, Toyonaka, Japan.
Odahara
T
T
Mitsuoka
K
K
Fujiyoshi
Y
Y
Murata
K
K
D001426:Bacterial Proteins / Q000648:ultrastructure; D001429:Bacteriochlorophylls / Q000737:chemistry; D003460:Crystallization; D045342:Light-Harvesting Protein Complexes; D008854:Microscopy, Electron; D045322:Photosynthetic Reaction Center Complex Proteins / Q000648:ultrastructure*; D011487:Protein Conformation; D012241:Rhodopseudomonas / Q000737:chemistry*
D001426:Bacterial Proteins; D001429:Bacteriochlorophylls; D045342:Light-Harvesting Protein Complexes; D045322:Photosynthetic Reaction Center Complex Proteins