title: A permanent hole burning study of the FMO antenna complex of the green sulfur bacterium Prosthecochloris aestuarii.
abstract: A permanent hole burning study on the Fenna-Matthews-Olson, or FMO, antenna complex of the green sulfur bacterium Prosthecochloris aestuarii was carried out at 6 K. Excitation resulted not only in relatively sharp features resonant with the burn wavelength but also in broad absorbance changes in the wavelength region of 800-820 nm. The shape of the latter changes was almost independent of the wavelength of excitation. Evidence is given that they are induced by a different mechanism than that which causes the resonant holes and that they may be due to a conformational change of the protein. The original spectrum was restored upon warming to 60 K. The effective dephasing times T2, as obtained from the homogeneous line widths, increased from about 0.5 ps at 803 nm to >/=20 ps at 830 nm and are in good agreement with recent measurements of accumulated photon-echo and time-resolved absorbance changes.
authors.affiliation: Department of Biophysics, Leiden University, P.O. Box 9504, 2300 RA Leiden, The Netherlands.
authors.forename: E M
authors.initials: EM
authors.lastname: Franken
authors.forename: S
authors.initials: S
authors.lastname: Neerken
authors.forename: R J
authors.initials: RJ
authors.lastname: Louwe
authors.forename: J
authors.initials: J
authors.lastname: Amesz
authors.forename: T J
authors.initials: TJ
authors.lastname: Aartsma
mesh_terms: D001426:Bacterial Proteins; D019414:Chlorobi / Q000528:radiation effects*; D004307:Dose-Response Relationship, Radiation; D004735:Energy Transfer; D007259:Infrared Rays; D007834:Lasers; D045342:Light-Harvesting Protein Complexes; D045322:Photosynthetic Reaction Center Complex Proteins / Q000528:radiation effects*; D011487:Protein Conformation; D013055:Spectrophotometry, Infrared
chemical_list: D001426:Bacterial Proteins; C086320:FMO bacteriochlorophyll protein, Bacteria; D045342:Light-Harvesting Protein Complexes; D045322:Photosynthetic Reaction Center Complex Proteins