Vasopressin neuron activation and Fos expression by stimulation of the caudal ventrolateral medulla.
The present study demonstrates that projections ascending from the caudal ventrolateral medulla have direct effects on the expression of the immediate early gene c-fos and of the arginine-vasopressin gene in neurosecretory cells of the hypothalamic supraoptic nucleus. Intense Fos-like immunoreactivity (Fos-LI) was observed in many magnocellular neurons of the supraoptic nucleus after electrical stimulation of the caudal ventrolateral medulla. In sham-operated rats, Fos-LI was absent or present in very few magnocellular neurons in the supraoptic nucleus. Fos-LI was visible in neurons expressing arginine-vasopressin, and was seen rarely in oxytocin neurons by double-immunostaining method. This study showed that 76% of all Fos-positive cells were arginine-vasopressin immunoreactive, whereas only 4% of them showed oxytocin immunoreactivity in the supraoptic nucleus. With in situ hybridization, a high level of arginine-vasopressin mRNA was noted in the supraoptic nucleus 3 h after stimulation of the caudal ventrolateral medulla; the expression was highest 6 h after the stimulation compared with the same region in sham-operated animals. These findings suggest that noradrenaline, released from the axon terminals originating from the caudal ventrolateral medulla, may participate in the regulation of gene transcription of arginine-vasopressin in response to physiological stimuli.
Shioda
S
S
Department of Anatomy, Showa University School of Medicine, Tokyo, Japan. shioda@med.showa-u.ac.jp
Iwase
M
M
Homma
I
I
Nakajo
S
S
Nakaya
K
K
Nakai
Y
Y
D000344:Afferent Pathways / Q000502:physiology; D000818:Animals; D001127:Arginine Vasopressin / Q000096:biosynthesis*; D001370:Axonal Transport; D004558:Electric Stimulation; D005786:Gene Expression Regulation / Q000502:physiology*; D016762:Genes, fos; D017403:In Situ Hybridization; D008297:Male; D008526:Medulla Oblongata / Q000502:physiology*; D009474:Neurons / Q000502:physiology*; D010835:Phytohemagglutinins; D016760:Proto-Oncogene Proteins c-fos / Q000096:biosynthesis*; D012333:RNA, Messenger / Q000096:biosynthesis; D051381:Rats; D017208:Rats, Wistar; D013495:Supraoptic Nucleus / Q000502:physiology*; D014158:Transcription, Genetic
D010835:Phytohemagglutinins; D016760:Proto-Oncogene Proteins c-fos; D012333:RNA, Messenger; C100156:leukoagglutinins, plants; D001127:Arginine Vasopressin