[Effect of L-glutamic acid on the reception of cytokines by HL-60 cells].
L-Glutamic acid at a concentration of 0.1 microM was found to induce differentiation of the cell line of HL-60 promyelocytic leukemia into granulocytes or neutrophils. The HL-60 cells have no specific glutamate-binding sites, but L-glutamic acid influences the reception of several cytokines by these cells. At a concentration of 0.1 microM, L-glutamic acid completely inhibits the high-affinity binding of 125I-labeled human recombinant interleukin-1 beta (Kd = 0.32 nM) to the HL-60 cells, but does not affect their low-affinity binding (Kd = 13.3 nM) and does not change the total number of the IL-1 beta-binding sites. Preincubation of the HL-60 cells with 0.1 microM of L-glutamic acid increases 2.5 times the number of receptors for 125I-labeled human recombinant tumor necrosis factor beta. These results suggest that L-glutamic acid plays an important role in the differentiation of the blood myeloid cells.
Kostanian
I A
IA
Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Moscow, Russia.
Nurieva
R I
RI
Novolotskaia
E V
EV
Astapova
M V
MV
Dranitsyna
S M
SM
Zav'ialov
V P
VP
Lipkin
V M
VM
D001667:Binding, Competitive; D002454:Cell Differentiation / Q000187:drug effects; D018698:Glutamic Acid / Q000378:metabolism / Q000494:pharmacology*; D006098:Granulocytes / Q000166:cytology / Q000187:drug effects*; D018922:HL-60 Cells; D006801:Humans; D007375:Interleukin-1 / Q000378:metabolism; D007457:Iodine Radioisotopes; D008233:Lymphotoxin-alpha / Q000378:metabolism; D009504:Neutrophils / Q000166:cytology / Q000187:drug effects*; D018121:Receptors, Cytokine / Q000187:drug effects / Q000378:metabolism*; D011994:Recombinant Proteins / Q000378:metabolism; D016339:Spectrometry, Mass, Fast Atom Bombardment
D007375:Interleukin-1; D007457:Iodine Radioisotopes; D008233:Lymphotoxin-alpha; D018121:Receptors, Cytokine; D011994:Recombinant Proteins; D018698:Glutamic Acid