Microscopical localization on adenylate cyclase: a historical review of methodologies.
The histochemistry technique for localizing adenylate cyclase has been developed over the past two decades. Early efforts were directed at overcoming the criticism of the lead capture technique, the inhibition of the enzyme by fixation, and problems associated with the substrate. The introduction of alternative metal ions, strontium and cerium, offered solutions to the criticism of the lead capture technique. The inhibition of the enzyme by the various fixation methods used has been rarely overcome satisfactorily and the use of non-fixed material during incubation is one of the alternatives that has been suggested. The introduction of adenylate (beta-gamma-methylene) diphosphate as an alternative substrate offers a solution to the problems associated with commercially available adenylyl imidodiphosphate. Although no standard medium or method has been accepted by all researchers, the histochemical technique still has a place in the arsenal of the modern cell biologist. The technique localizes the active enzyme, as opposed to the protein, active and nonactive, by immunocytochemistry and the precursors of the protein by in situ hybridization methods.
Richards
P A
PA
Department of Anatomy, University of Pretoria, Sovenga, Republic of South Africa.
Richards
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PD
D000255:Adenosine Triphosphate / Q000031:analogs & derivatives / Q000032:analysis; D000262:Adenylyl Cyclases / Q000032:analysis*; D000266:Adenylyl Imidodiphosphate / Q000032:analysis; D000818:Animals; D006651:Histocytochemistry / Q000266:history / Q000379:methods*; D049673:History, 20th Century; D016707:Tissue Fixation
D000266:Adenylyl Imidodiphosphate; C005147:5'-adenylyl (beta,gamma-methylene)diphosphonate; D000255:Adenosine Triphosphate; D000262:Adenylyl Cyclases; C002630:alpha,beta-methyleneadenosine 5'-triphosphate