Inhibition of the specific 3H-DL-Glu binding in the hippocampus of rat brain by lead.
The effect of Pb2+ on 3H-DL-Glutamate (3H-DL-Glu) binding in the membrane preparations from the hippocampus of rat brain was investigated with a view to explaining the cognitive and learning deficits produced by the heavy metal. The results indicated that Pb2+ (3.1-25.0 mumol.L-1) inhibited 3H-DL-Glu binding in a concentration-dependent manner. Scatchard analysis further revealed that at a concentration of 6.3 mumol.L-1 Pb2+ interfered with binding mainly through significantly decreasing the density of binding sites. This finding provided an important insight into Pb2(+)-induced impairments in learning and memory previously documented in children and in experimental animals chronically exposed to Pb2+.
Zhang
H S
HS
Department of Toxicology, Chinese Academy of Preventive Medicine, Beijing, China.
Zhao
X L
XL
Song
L H
LH
Qin
Y H
YH
D000818:Animals; D001665:Binding Sites / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D005973:Glutamine / Q000378:metabolism*; D006624:Hippocampus / Q000187:drug effects / Q000378:metabolism*; D007854:Lead / Q000633:toxicity*; D007858:Learning / Q000187:drug effects; D008297:Male; D008568:Memory / Q000187:drug effects; D009942:Organometallic Compounds / Q000008:administration & dosage / Q000633:toxicity*; D051381:Rats; D017208:Rats, Wistar; D013237:Stereoisomerism; D014316:Tritium
D009942:Organometallic Compounds; D005973:Glutamine; D014316:Tritium; D007854:Lead; C008261:lead acetate