title: Release of the soluble interleukin-6 receptor from human T-cells.
abstract: The soluble Interleukin-6 receptor (sIL-6R) is capable of conferring the Interleukin-6 (IL-6) signal onto cells lacking the gp80 ligand binding protein. Here we investigate the release of sIL-6R from T-cells. After 2 h stimulation with PMA, a release of sIL-6R from peripheral human T-cells was observed which was insensitive to the protein synthesis inhibitor cycloheximide. This release was accompanied by a decrease of membrane-bound (mb) IL-6R. After 24 h, however, the observed sIL6-R release did prove to be sensitive to cycloheximide. These results suggest that both shedding and denovo-synthesis may be responsible for the PMA-induced sIL-6R release. In contrast to PMA, neither anti-CD3, a positive, nor IL-10, a negative regulator of IL-6 release from T-cells affected the production of the sIL-6R. The differential regulation of sIL-6R and IL-6 production by T-cells might be relevant for the immunomodulatory potential of the sIL-6R with respect to the interaction of T- and non-T-cells.
authors.affiliation: Department of Pediatric Hematology and Oncology, Heinrich-Heine University, Düsseldorf, Germany.
authors.forename: U
authors.initials: U
authors.lastname: Banning
authors.forename: H
authors.initials: H
authors.lastname: Bönig
authors.forename: B
authors.initials: B
authors.lastname: Pafferath
authors.forename: A
authors.initials: A
authors.lastname: Klein-Vehne
authors.forename: S
authors.initials: S
authors.lastname: Burdach
authors.forename: D
authors.initials: D
authors.lastname: Körholz
mesh_terms: D017252:CD3 Complex / Q000378:metabolism; D003513:Cycloheximide / Q000494:pharmacology; D006801:Humans; D016753:Interleukin-10 / Q000494:pharmacology; D008934:Mitogens / Q000494:pharmacology; D011500:Protein Synthesis Inhibitors / Q000494:pharmacology; D019947:Receptors, Interleukin-6 / Q000378:metabolism*; D012995:Solubility; D013601:T-Lymphocytes / Q000187:drug effects / Q000378:metabolism*; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology
chemical_list: D017252:CD3 Complex; D008934:Mitogens; D011500:Protein Synthesis Inhibitors; D019947:Receptors, Interleukin-6; D016753:Interleukin-10; D003513:Cycloheximide; D013755:Tetradecanoylphorbol Acetate