Role for IL-15/IL-15 receptor beta-chain in natural killer 1.1+ T cell receptor-alpha beta+ cell development.
Mouse TCR alphabeta+ cells expressing NKR-P1 (NK1+ T cells in the C57BL/6 strain) are classified as a unique subset that require beta2m-associated, MHC class I-like molecules for development and preferentially express particular V beta and V alphaJ domains of the TCR. We show here that NK1+ T cells express the IL-2R beta/IL-15R beta that is normally present on conventional NK cells, but not T cells. In mice lacking IL-2R beta/IL-15R beta chain, the number of NK1+ T cells is dramatically reduced. However, in IL-2-deficient mice, NK1+ T cells develop normally. Moreover, NK1+ T cells proliferate upon stimulation by IL-15, and the proliferation is blocked by the addition of anti-IL-2R beta/IL-15R beta Abs. Collectively, our data indicate that NK1+ T cells require IL-2R beta/IL-15R beta chain in an IL-2-independent manner and demonstrate that IL-15 plays a crucial role during development.
Ohteki
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T
Department of Medical Biophysics, Ontario Cancer Institute, Toronto, Canada.
Ho
S
S
Suzuki
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Mak
T W
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Ohashi
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PS
D000818:Animals; D000941:Antigens / Q000276:immunology*; D000954:Antigens, Surface; D002454:Cell Differentiation / Q000187:drug effects / Q000235:genetics / Q000276:immunology; D002455:Cell Division / Q000187:drug effects / Q000276:immunology; D053590:Interleukin 1 Receptor Antagonist Protein; D019409:Interleukin-15 / Q000378:metabolism / Q000502:physiology*; D037181:Lectins, C-Type; D051379:Mice; D008810:Mice, Inbred C57BL; D008817:Mice, Mutant Strains; D055651:NK Cell Lectin-Like Receptor Subfamily B; D011506:Proteins / Q000276:immunology*; D016693:Receptors, Antigen, T-Cell, alpha-beta / Q000276:immunology*; D017472:Receptors, Interleukin-1 / Q000037:antagonists & inhibitors; D053647:Receptors, Interleukin-15; D015375:Receptors, Interleukin-2 / Q000096:biosynthesis / Q000235:genetics / Q000502:physiology*; D012795:Sialoglycoproteins / Q000096:biosynthesis; D016176:T-Lymphocyte Subsets / Q000166:cytology / Q000378:metabolism*
D000941:Antigens; D000954:Antigens, Surface; C508025:Il15ra protein, mouse; C507764:Il1rn protein, mouse; D053590:Interleukin 1 Receptor Antagonist Protein; D019409:Interleukin-15; D037181:Lectins, C-Type; D055651:NK Cell Lectin-Like Receptor Subfamily B; D011506:Proteins; D016693:Receptors, Antigen, T-Cell, alpha-beta; D017472:Receptors, Interleukin-1; D053647:Receptors, Interleukin-15; D015375:Receptors, Interleukin-2; D012795:Sialoglycoproteins