CD40 and IL-4 regulate murine CD27L expression.
It is well known that interactions between accessory molecules on T cells and their ligands on APC play a key role in regulating T cell effector activity. The factors controlling the expression of these molecules are thus important determinants in the outcome of T cell activation. We have examined the expression of the murine ligand for CD27, a costimulatory molecule on T cells. Evidence is shown that CD27L is expressed at a low level on resting B cells but not on T cells, and that activation of B cells by culture with LPS or anti-IgM Ab increases the expression of CD27L. Interestingly, coligation of CD40 down-regulates CD27L on LPS-activated B cells but not on anti-Ig-activated cells. These findings suggest that costimulation via the CD27-CD27L pathway may be limited to interactions involving Ag-specific B cells, i.e., B cells specifically activated via their Ig receptors. In addition, testing a spectrum of different cytokines indicated that IL-4 and TGF, but not IL-2, IL-10, or IFN-gamma, prevented up-regulation of CD27L expression on activated B cells even when activation was induced by Ig signaling. The capacity of IL-4 to prevent CD27L expression could thus serve to limit CD27-CD27L interactions to Th1-type T cell responses.
Hartwig
U F
UF
Department of Immunology, The Scripps Research Institute, La Jolla, CA 92037, USA.
Karlsson
L
L
Peterson
P A
PA
Webb
S R
SR
D000818:Animals; D000888:Antibodies, Anti-Idiotypic / Q000494:pharmacology; D015703:Antigens, CD / Q000378:metabolism; D001402:B-Lymphocytes / Q000378:metabolism; D053282:CD27 Ligand; D019013:CD40 Antigens / Q000502:physiology*; D016207:Cytokines / Q000494:pharmacology; D015536:Down-Regulation / Q000276:immunology; D006801:Humans; D007074:Immunoglobulin G / Q000235:genetics; D007075:Immunoglobulin M / Q000276:immunology; D015847:Interleukin-4 / Q000502:physiology*; D008024:Ligands; D008070:Lipopolysaccharides / Q000494:pharmacology; D008213:Lymphocyte Activation; D008565:Membrane Proteins / Q000096:biosynthesis* / Q000235:genetics / Q000276:immunology; D051379:Mice; D008810:Mice, Inbred C57BL; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis; D012995:Solubility
D000888:Antibodies, Anti-Idiotypic; D015703:Antigens, CD; D053282:CD27 Ligand; D019013:CD40 Antigens; C505771:CD70 protein, human; C505772:Cd70 protein, mouse; D016207:Cytokines; D007074:Immunoglobulin G; D007075:Immunoglobulin M; D008024:Ligands; D008070:Lipopolysaccharides; D008565:Membrane Proteins; D011993:Recombinant Fusion Proteins; C044203:anti-IgM; D015847:Interleukin-4