Rapid method for isolation of total RNA from eukaryotic cell lines and leukocytes.
Total RNA was isolated from human leukocytes (monocytes, granulocytes), various cell lines (COS-7, Mono-Mac-6, L-132, HaCaT, EA.hy926, HL-60), and fungal mycelium by a rapid two-step method. Cells were lysed with NaDodSO4 in a citric acid-containing buffer. This procedure was succeeded by salt precipitation to remove contaminating DNA and protein and a final alcohol precipitation of RNA. Isolated RNA was of high quality, with a reasonable yield and little or no protein or DNA contamination. We present here a fast method for preparing RNA particularly from cell lines, which limits the use of toxic compounds.
Dreier
J
J
Institute of Experimental Dermatology, University of Münster, Germany.
Högger
P
P
Sorg
C
C
D000818:Animals; D019556:COS Cells; D002460:Cell Line; D005057:Eukaryotic Cells / Q000737:chemistry*; D005658:Fungi / Q000737:chemistry; D006801:Humans; D007962:Leukocytes / Q000737:chemistry*; D012313:RNA / Q000302:isolation & purification*
D012313:RNA