Stereoselective interaction of drug enantiomers with human serum transferrin in capillary zone electrophoresis (II).
Further studies on chiral resolution of drugs with different chemical structures by capillary zone electrophoresis using iron-free human serum transferrin are described. The substances passed a highly concentrated pseudo-stationary protein zone applied in a coated capillary and the possible chiral separation of the optical isomers was followed. Eighteen drugs with different structures were screened, and the enantiomers of clofedanol, buphenine, acebutolol and chlorphenamine were resolved. Several, but not all drugs, showed longer migration times while passing the protein zone, indicating an interaction with transferrin, although chiral resolution was not observed in all cases. The observations provided further information about the properties of the surface interaction sites of transferrin.
Schmid
M G
MG
Institute of Pharmaceutical Chemistry, Karl-Franzens University of Graz, Austria.
Gübitz
G
G
Kilár
F
F
D019075:Electrophoresis, Capillary / Q000379:methods*; D006801:Humans; D015394:Molecular Structure; D004364:Pharmaceutical Preparations / Q000737:chemistry / Q000302:isolation & purification; D013237:Stereoisomerism; D014168:Transferrin / Q000737:chemistry*
D004364:Pharmaceutical Preparations; D014168:Transferrin