Embryonic implantation in galectin 1/galectin 3 double mutant mice.
Galectin 1 and galectin 3 are first expressed in the trophectoderm cells of the implanting embryo and have been implicated in the process of implantation. However, we had previously shown that the lack of galectin 1 in galectin 1 null mutant mice is compatible with implantation. In this study, we describe the generation of galectin 3 null mutant mice and show that they are viable and have no overt abnormalities. The importance of galectin 1 and galectin 3 in implantation was assessed by obtaining double mutant mice [gal1 -/-; gal3 -/-]. We find that implantation can still occur in the absence of both galectin 1 and galectin 3. However, we show that galectin 5, a third member of this gene family, is also present in the blastocyst at the time of implantation.
Colnot
C
C
Unité INSERM 257, ICGM, Paris, France.
Fowlis
D
D
Ripoche
M A
MA
Bouchaert
I
I
Poirier
F
F
D000818:Animals; D000943:Antigens, Differentiation / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D001755:Blastocyst / Q000378:metabolism; D002478:Cells, Cultured; D003001:Cloning, Molecular; D010064:Embryo Implantation / Q000502:physiology*; D005260:Female; D019084:Fluorescent Antibody Technique, Indirect; D037483:Galectin 1; D037502:Galectin 3; D037161:Galectins; D006388:Hemagglutinins / Q000378:metabolism / Q000502:physiology*; D037102:Lectins / Q000378:metabolism / Q000502:physiology; D051379:Mice; D018345:Mice, Knockout; D018613:Microscopy, Confocal
D000943:Antigens, Differentiation; D037483:Galectin 1; D037502:Galectin 3; D037161:Galectins; D006388:Hemagglutinins; D037102:Lectins; C092501:galectin 5