Enhanced prevalence of T cells expressing TCRBV8S2 and TCRBV8S3 in hearts of chronically Trypanosoma cruzi-infected mice.
We have analysed the relative T cell receptor (TCR) BV gene usage in T cells from hearts and spleens of CBA/HJ mice chronically infected with the Tulahuén strain of Trypanosoma cruzi. During chronic infection, CBA/HJ mice recruit T cells at the major site of inflammation (i.e. the heart), with over-representation of certain TCRBV gene subfamilies (TCRBV8S2 and TCRBV8S3). In contrast, no signal or a very weak message from a limited number of T cells was recorded from one heart of the control group. No alteration of TCRBV distribution was recorded in spleens of chronically infected CBA/HJ. Our findings indicate that there is a preferential TCRBV gene usage in the T cell response in the hearts of chronically infected mice. Furthermore, the pattern of CDR3 lengths in inflammatory T cells was altered.
Sunnemark
D
D
Microbiology and Tumorbiology Center, Karolinska Institutet, Stockholm, Sweden. Dan.Sunnemark@mtc.ki.se
Andersson
R
R
Harris
R A
RA
Jeddi-Tehrani
M
M
Orn
A
A
D000818:Animals; D014355:Chagas Disease / Q000276:immunology* / Q000473:pathology; D019674:Genes, T-Cell Receptor beta; D006321:Heart / Q000469:parasitology*; D007135:Immunoglobulin Variable Region / Q000096:biosynthesis* / Q000235:genetics; D051379:Mice; D008808:Mice, Inbred CBA; D009206:Myocardium / Q000276:immunology* / Q000473:pathology; D016693:Receptors, Antigen, T-Cell, alpha-beta / Q000096:biosynthesis* / Q000235:genetics / Q000276:immunology; D013601:T-Lymphocytes / Q000276:immunology*; D014349:Trypanosoma cruzi
D007135:Immunoglobulin Variable Region; D016693:Receptors, Antigen, T-Cell, alpha-beta