title: P- and E-selectins are required for the leukocyte recruitment, but not the tissue swelling, associated with IgE- and mast cell-dependent inflammation in mouse skin.
abstract: Many studies, in both experimental animal and human systems, have indicated that P- and/or E-selectins may contribute importantly to the leukocyte recruitment that occurs in association with mast cell-dependent inflammatory responses. We used mice that genetically lack P-selectin (P -/-), E-selectin (E -/-), or both selectins (P/E -/-) to investigate the possible roles of these selectins in the IgE- and mast cell-dependent recruitment of neutrophils to the skin of mice. We found that a lack of either or both selectins had little or no effect on the extent of mast cell degranulation or the tissue swelling associated with these reactions. Moreover, a lack of either P- or E-selectin alone did not reduce the neutrophil infiltration at the reaction sites. However, mice lacking both P- and E-selectins exhibited an almost complete ablation of IgE- and mast cell-dependent neutrophil recruitment. These findings show that P- and E-selectins can express overlapping functions in leukocyte recruitment associated with IgE- and mast cell-dependent cutaneous late-phase reactions in mouse skin, and that a lack of both selectins results in a virtual elimination of IgE-dependent leukocyte recruitment.
authors.affiliation: Department of Pathology, Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, Massachusetts 02215, USA.
authors.forename: F
authors.initials: F
authors.lastname: de Mora
authors.forename: C M
authors.initials: CM
authors.lastname: Williams
authors.forename: P S
authors.initials: PS
authors.lastname: Frenette
authors.forename: D D
authors.initials: DD
authors.lastname: Wagner
authors.forename: R O
authors.initials: RO
authors.lastname: Hynes
authors.forename: S J
authors.initials: SJ
authors.lastname: Galli
mesh_terms: D000818:Animals; D015550:Cell Degranulation; D003872:Dermatitis / Q000209:etiology*; D019040:E-Selectin / Q000502:physiology*; D005260:Female; D007073:Immunoglobulin E / Q000502:physiology*; D008407:Mast Cells / Q000502:physiology*; D051379:Mice; D008810:Mice, Inbred C57BL; D009504:Neutrophils / Q000502:physiology*; D019007:P-Selectin / Q000502:physiology*; D010323:Passive Cutaneous Anaphylaxis
chemical_list: D019040:E-Selectin; D019007:P-Selectin; D007073:Immunoglobulin E