mRNA distribution in adult human brain of GRIN2B, a N-methyl-D-aspartate (NMDA) receptor subunit.
The expression of the N-methyl-D-aspartate (NMDA) receptor subunit NR2B/epsilon2 (GRIN2B) in the human adult brain was assayed by in situ hybridisation, by using a specific cRNA probe. The full length GRIN2B cDNA was cloned and sequenced. It showed a 90% nucleotide conservation when compared to the rodent homologue. GRIN2B gene is expressed at high levels in the fronto-parieto-temporal cortex and hippocampus pyramidal cells and, at a lower extent, in the basal ganglia (amygdala and striatum). The cerebellar granule cells does not show any mRNA expression. The non-ubiquitous anatomical distribution of the GRIN2B mRNA in the central nervous system suggests that the gene could be involved in specific functions pertaining to the expressing cell groups.
Schito
A M
AM
Institute of Biology and Genetics, University of Genoa, Italy.
Pizzuti
A
A
Di Maria
E
E
Schenone
A
A
Ratti
A
A
Defferrari
R
R
Bellone
E
E
Mancardi
G L
GL
Ajmar
F
F
Mandich
P
P
D000328:Adult; D001923:Brain Chemistry / Q000502:physiology*; D002874:Chromosome Mapping; D006801:Humans; D017403:In Situ Hybridization; D010446:Peptide Fragments / Q000032:analysis*; D016133:Polymerase Chain Reaction / Q000379:methods; D012333:RNA, Messenger / Q000032:analysis*; D016194:Receptors, N-Methyl-D-Aspartate / Q000032:analysis* / Q000737:chemistry; D012016:Reference Values; D014158:Transcription, Genetic
D010446:Peptide Fragments; D012333:RNA, Messenger; D016194:Receptors, N-Methyl-D-Aspartate