Characterization of marker chromosomes by fish using microdissected probes from old Carnoy-fixed cells: report of two cases.
We reported on two patients with a de novo marker chromosome of which the origins were successfully identified by FISH using microdissected probes. These probes were established by microdissections of extra chromosomal segments from Carnoy-fixed cells stored at -20 degrees C for several years. Using these probes, we could verify partial 1q32 trisomy in a patient with 17p+ as well as partial 16q2 trisomy in another patient with 4p+.
Chinen
Y
Y
Department of Pediatrics, University of the Ryukyus School of Medicine, Okinawa, Japan.
Tohma
T
T
Izumikawa
Y
Y
Naritomi
K
K
Hirayama
K
K
D000015:Abnormalities, Multiple / Q000235:genetics; D019342:Acetic Acid; D002675:Child, Preschool; D002871:Chromosome Banding; D002886:Chromosomes, Human, Pair 17; D002894:Chromosomes, Human, Pair 4; D015342:DNA Probes; D000431:Ethanol; D005260:Female; D005404:Fixatives; D005819:Genetic Markers; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D008607:Intellectual Disability / Q000235:genetics; D016133:Polymerase Chain Reaction
D015342:DNA Probes; D005404:Fixatives; D005819:Genetic Markers; D000431:Ethanol; D019342:Acetic Acid