Flow injection immunoanalysis based on a magnetoimmunosensor system.
A new immunosensor integrated to a flow system has been developed. It is based on magnetic immunoparticles immobilized on a solid-state transducer using a magnetic field. The described technique renews the immunoparticles reproducibly for each analysis allowing a good measurement precision. The developed experimental approach permits the implementation of an automated immunoassay that is quick (analytical cycle < 30 min) and sensitive in the micromolar concentration range. The system was applied to the determination of rabbit immunoglobulin G as an analyte model.
Solé
S
S
Departament de Química, Universitat Autònoma de Barcelona, Bellaterra, Catalonia, Spain.
Alegret
S
S
Céspedes
F
F
Fàbregas
E
E
Díez-Caballero
T
T
D000818:Animals; D000918:Antibody Specificity; D002021:Buffers; D002138:Calibration; D017022:Flow Injection Analysis / Q000379:methods*; D007124:Immunoenzyme Techniques; D007074:Immunoglobulin G / Q000032:analysis*; D008280:Magnetics; D010316:Particle Size; D011817:Rabbits; D015203:Reproducibility of Results; D014510:Urease / Q000737:chemistry
D002021:Buffers; D007074:Immunoglobulin G; D014510:Urease