title: Hydroxyl radical formation resulting from the interaction of nitric oxide and hydrogen peroxide.
abstract: The highly reactive and cytotoxic hydroxyl radical (OH) was found by electrochemical detection to be produced in reactions involving hydrogen peroxide (H2O2) and the nitric oxide (NO) donor diethylamine- NO complex. Using aromatic hydroxylation of salicylate as a specific indicator of OH, three salicylate hydroxylation products were identified; catechol, 2,3- and 2,5-dihydroxybenzoic acid. Four additional compounds were detected but not identified. The interactions of H2O2 and NO represent a biologically feasible reaction mechanism that can account for OH-induced damage in cellular environments where transition metal ions are unavailable for participation in the superoxide-mediated Fenton reaction. The ability of the NO/H2O2 complex to generate OH independently of iron or other transition metals provides a new focus for studies concerned with the origin of tissue-specific damage caused by oxygen-derived species.
authors.affiliation: Department of Biology, Loyola University Chicago, IL 60626, USA. anappi@orion.it.luc.edu
authors.forename: A J
authors.initials: AJ
authors.lastname: Nappi
authors.forename: E
authors.initials: E
authors.lastname: Vass
mesh_terms: D004563:Electrochemistry; D006861:Hydrogen Peroxide / Q000737:chemistry*; D017665:Hydroxyl Radical / Q000737:chemistry*; D006900:Hydroxylation; D066298:In Vitro Techniques; D007501:Iron / Q000737:chemistry; D009569:Nitric Oxide / Q000737:chemistry*; D010084:Oxidation-Reduction; D018384:Oxidative Stress; D017382:Reactive Oxygen Species; D012459:Salicylates / Q000737:chemistry; D020156:Salicylic Acid
chemical_list: D017382:Reactive Oxygen Species; D012459:Salicylates; D009569:Nitric Oxide; D017665:Hydroxyl Radical; D006861:Hydrogen Peroxide; D007501:Iron; D020156:Salicylic Acid