title: mRNA distribution in adult human brain of GRIN2B, a N-methyl-D-aspartate (NMDA) receptor subunit.
abstract: The expression of the N-methyl-D-aspartate (NMDA) receptor subunit NR2B/epsilon2 (GRIN2B) in the human adult brain was assayed by in situ hybridisation, by using a specific cRNA probe. The full length GRIN2B cDNA was cloned and sequenced. It showed a 90% nucleotide conservation when compared to the rodent homologue. GRIN2B gene is expressed at high levels in the fronto-parieto-temporal cortex and hippocampus pyramidal cells and, at a lower extent, in the basal ganglia (amygdala and striatum). The cerebellar granule cells does not show any mRNA expression. The non-ubiquitous anatomical distribution of the GRIN2B mRNA in the central nervous system suggests that the gene could be involved in specific functions pertaining to the expressing cell groups.
authors.affiliation: Institute of Biology and Genetics, University of Genoa, Italy.
authors.forename: A M
authors.initials: AM
authors.lastname: Schito
authors.forename: A
authors.initials: A
authors.lastname: Pizzuti
authors.forename: E
authors.initials: E
authors.lastname: Di Maria
authors.forename: A
authors.initials: A
authors.lastname: Schenone
authors.forename: A
authors.initials: A
authors.lastname: Ratti
authors.forename: R
authors.initials: R
authors.lastname: Defferrari
authors.forename: E
authors.initials: E
authors.lastname: Bellone
authors.forename: G L
authors.initials: GL
authors.lastname: Mancardi
authors.forename: F
authors.initials: F
authors.lastname: Ajmar
authors.forename: P
authors.initials: P
authors.lastname: Mandich
mesh_terms: D000328:Adult; D001923:Brain Chemistry / Q000502:physiology*; D002874:Chromosome Mapping; D006801:Humans; D017403:In Situ Hybridization; D010446:Peptide Fragments / Q000032:analysis*; D016133:Polymerase Chain Reaction / Q000379:methods; D012333:RNA, Messenger / Q000032:analysis*; D016194:Receptors, N-Methyl-D-Aspartate / Q000032:analysis* / Q000737:chemistry; D012016:Reference Values; D014158:Transcription, Genetic
chemical_list: D010446:Peptide Fragments; D012333:RNA, Messenger; D016194:Receptors, N-Methyl-D-Aspartate