Solid-phase extraction cleanup for ivermectin in liver tissue.
Extraction of liver tissue with organic solvent produces coextractants with compounds of interest. The solid-phase extraction (SPE) cleanup of liver tissue developed for ivermectin removes nonpolar coextractants. Liver extract that has been reduced to dryness is reconstituted in 0.5 mL acetonitrile. The mixture is passed through 0.1 g C18 SPE column, and the eluate is collected. The column is eluted further with 2 mL acetonitrile. Combined eluates are derivatized with 1-methylimidazole and trifluoroacetic anhydride, and the ivermectin derivative is determined by liquid chromatography with fluorescence detection.
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U.S. Department of Agriculture, Food Safety and Inspection Service, Eastern Laboratory, Richard B. Russell Center, Athens, GA 30604, USA.
Di Benedetto
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D000818:Animals; D000871:Anthelmintics / Q000032:analysis*; D007202:Indicators and Reagents; D007559:Ivermectin / Q000031:analogs & derivatives / Q000032:analysis*; D008099:Liver / Q000737:chemistry*; D012015:Reference Standards
D000871:Anthelmintics; D007202:Indicators and Reagents; C048324:abamectin; D007559:Ivermectin