A cognate tRNA specific conformational change in glutaminyl-tRNA synthetase and its implication for specificity.
Conformational changes that occur upon substrate binding are known to play crucial roles in the recognition and specific aminoacylation of cognate tRNA by glutaminyl-tRNA synthetase. In a previous study we had shown that glutaminyl-tRNA synthetase labeled selectively in a nonessential sulfhydryl residue by an environment sensitive probe, acrylodan, monitors many of the conformational changes that occur upon substrate binding. In this article we have shown that the conformational change that occurs upon tRNA(Gln) binding to glnRS/ATP complex is absent in a noncognate tRNA tRNA(Glu)-glnRS/ATP complex. CD spectroscopy indicates that this cognate tRNA(Gln)-induced conformational change may involve only a small change in secondary structure. The Van't Hoff plot of cognate and noncognate tRNA binding in the presence of ATP is similar, suggesting similar modes of interaction. It was concluded that the cognate tRNA induces a local conformational change in the synthetase that may be one of the critical elements that causes enhanced aminoacylation of the cognate tRNA over the noncognate ones.
Mandal
A K
AK
Department of Biophysics, Bose Institute, Calcutta, India.
Bhattacharyya
A
A
Bhattacharyya
S
S
Bhattacharyya
T
T
Roy
S
S
D015081:2-Naphthylamine / Q000031:analogs & derivatives / Q000378:metabolism; D000255:Adenosine Triphosphate / Q000378:metabolism; D000604:Amino Acyl-tRNA Synthetases / Q000737:chemistry*; D002942:Circular Dichroism; D005453:Fluorescence; D011485:Protein Binding / Q000502:physiology; D011487:Protein Conformation; D017433:Protein Structure, Secondary; D012351:RNA, Transfer, Gln / Q000378:metabolism*; D012352:RNA, Transfer, Glu / Q000378:metabolism; D013379:Substrate Specificity; D013696:Temperature
D012351:RNA, Transfer, Gln; D012352:RNA, Transfer, Glu; C038518:acrylodan; D000255:Adenosine Triphosphate; D015081:2-Naphthylamine; D000604:Amino Acyl-tRNA Synthetases; C020659:glutaminyl-tRNA synthetase