title: Experimental infection of domestic sheep with culture-derived Leishmania donovani promastigotes.
abstract: Domestic sheep were intradermally inoculated with culture-derived stationary phase Leishmania donovani promastigotes. Sampling of site of inoculation, liver and spleen for 244 days showed that this parasite can stay alive in the skin for up to 28 days post-inoculation. Apart from pyrexia that was evident in all the animals for 42 days, no other symptoms of kala-azar were seen. No parasites were recovered from the visceral organs throughout the sampling period, suggesting that sheep are not susceptible to infection with L. donovani. It is therefore unlikely that sheep can be synanthropic reservoirs for this parasite.
authors.affiliation: Biomedical Sciences Research Center, Kenya Medical Research Institute, Nairobi, Kenya. kemrilib@ken.healthnet.org
authors.forename: C O
authors.initials: CO
authors.lastname: Anjili
authors.forename: C K
authors.initials: CK
authors.lastname: Ngichabe
authors.forename: P A
authors.initials: PA
authors.lastname: Mbati
authors.forename: R M
authors.initials: RM
authors.lastname: Lugalia
authors.forename: H M
authors.initials: HM
authors.lastname: Wamwayi
authors.forename: J I
authors.initials: JI
authors.lastname: Githure
mesh_terms: D000818:Animals; D000913:Antibodies, Protozoan / Q000097:blood; D004197:Disease Reservoirs; D004198:Disease Susceptibility; D004797:Enzyme-Linked Immunosorbent Assay / Q000662:veterinary; D007271:Injections, Intradermal / Q000662:veterinary; D007630:Kenya; D007893:Leishmania donovani / Q000276:immunology / Q000502:physiology*; D007898:Leishmaniasis, Visceral / Q000469:parasitology / Q000662:veterinary*; D008099:Liver / Q000469:parasitology; D008297:Male; D012756:Sheep; D012757:Sheep Diseases / Q000469:parasitology*; D012867:Skin / Q000469:parasitology; D013154:Spleen / Q000469:parasitology
chemical_list: D000913:Antibodies, Protozoan