title: Determination of cyclohexamone after derivatization with 2,4-dinitrophenyl hydrazine in intravenous solutions stored in PVC bags by high performance liquid chromatography.
abstract: A high performance liquid chromatographic procedure is described for the determination of cyclohexanone leached in intravenous solutions from the poly(vinyl chloride) bags. After derivatization with 2,4-dinitrophenylhydrazine and extraction with pentane, the cyclohexanone derivative was analysed on a C18 BDS Hypersil column using mobile phase mixture of acetonitrile:water (55:45). Ultra-violet detection was performed at 368 nm. The limit of quantification was 30 ng/mL and the assay was linear from 0.05 to 50 micrograms/mL. The recovery was better than 95%. The proposed method is satisfactory in its accuracy and precision with particularly relative standard deviations (RSD) for intra-assay and inter-assay of below 10%. This method has been successfully used for the determination of cyclohexanone in aqueous solutions such as sodium chloride (0.9%) and glucose (5%) stored in PVC containers. The values obtained varied between 2.04 and 44.9 micrograms/mL according to solutions and volume.
authors.affiliation: Laboratoire de Pharmacologie, Pharmacocinétique et Pharmacie Clinique, Faculté de Pharmacie, Lille, France.
authors.forename: F
authors.initials: F
authors.lastname: Khalfi
authors.forename: T
authors.initials: T
authors.lastname: Dine
authors.forename: M
authors.initials: M
authors.lastname: Luyckx
authors.forename: B
authors.initials: B
authors.lastname: Gressier
authors.forename: C
authors.initials: C
authors.lastname: Brunet
authors.forename: L
authors.initials: L
authors.lastname: Ballester
authors.forename: M
authors.initials: M
authors.lastname: Cazin
authors.forename: J C
authors.initials: JC
authors.lastname: Cazin
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D003512:Cyclohexanones / Q000032:analysis* / Q000737:chemistry; D007262:Infusions, Intravenous; D010659:Phenylhydrazines / Q000737:chemistry*; D011143:Polyvinyl Chloride; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D012996:Solutions / Q000737:chemistry*; D013056:Spectrophotometry, Ultraviolet
chemical_list: D003512:Cyclohexanones; D010659:Phenylhydrazines; D012996:Solutions; C004787:2,4-dinitrophenylhydrazine; C036468:cyclohexanone; D011143:Polyvinyl Chloride