Influence of Clostridium botulinum C2 toxin on Fc epsilonRI-mediated secretion and tyrosine phosphorylation in RBL cells.
We studied the effects of the binary Clostridium botulinum C2 toxin on stimulated [3H]serotonin release and protein tyrosine phosphorylation in RBL 2H3 hm1 cells. Actin was specifically ADP-ribosylated by C2 toxin in intact cells resulting in a 2-3 fold increase in antigen- or calcium ionophore (A23187)-induced degranulation. The effects of C2 toxin were time- and concentration-dependent. Toxin treatment, which dramatically changes the morphology of RBL cells, was not sufficient to induce mediator release in the absence of activators of secretion. Antigen- and A23187-stimulated tyrosine phosphorylation of 60-80 kDa and 110-120 kDa proteins was reduced or blocked after C2 toxin incubation. Treatment of RBL cells with the tyrosine phosphatase inhibitor pervanadate reversed the inhibitory effect of C2 toxin on stimulated protein tyrosine phosphorylation indicating activation of phosphatases by C2 toxin. The data indicate that disassembly of the actin cytoskeleton by C2 toxin facilitates Fc epsilonRI-mediated signal-secretion coupling and suggest a role of the actin cytoskeleton in phosphatase regulation in RBL cells.
Prepens
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Institut für Pharmakologie und Toxikologie der Albert-Ludwigs-Universität Freiburg, Germany.
Barth
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Wilting
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J
Aktories
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K
D000199:Actins / Q000187:drug effects / Q000378:metabolism; D000818:Animals; D001905:Botulinum Toxins / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D015471:Leukemia, Basophilic, Acute / Q000378:metabolism* / Q000473:pathology; D010766:Phosphorylation; D051381:Rats; D017455:Receptors, IgE / Q000187:drug effects* / Q000378:metabolism; D012701:Serotonin / Q000378:metabolism; D014407:Tumor Cells, Cultured / Q000187:drug effects / Q000648:ultrastructure; D014443:Tyrosine / Q000378:metabolism*
D000199:Actins; D017455:Receptors, IgE; D012701:Serotonin; D014443:Tyrosine; D001905:Botulinum Toxins; C096324:botulinum toxin type C