title: Protection of UV-induced suppression of skin contact hypersensitivity: a common feature of flavonoids after oral administration?
abstract: In this study we investigated the effect of the dietary ingredients fruit and vegetable, green tea phenol extract (GTP) and the specific flavonoid components quercetin and chrysin on the UV-induced suppression of the contact hypersensitivity (CHS) response to picryl chloride (PCl). The SKH-1 mice were fed with test diet from 2 or 4 weeks before and during the UV irradiation (daily, 95 mJ/cm2) and tested for the CHS ear-swelling response 10 weeks after the onset of the irradiation. For the CHS, mice were immunized with PCl by epicutaneous application on nonirradiated sites. Four days after sensitization all mice were challenged on both sides of each ear by topical application of one drop PCl. In addition, from mice fed with the fruit and vegetable mixture the number of Langerhans cells (LC) were scored in the skin and from mice fed with quercetin, quercetin levels in plasma were measured at week 11 after the start of UV irradiation. It was found that fruit and vegetable (19% in the diet), GTP (0.1% and 0.01% in the drinking water), quercetin (1% in the diet) and chrysin (1% and 0.1% in the diet), prevented statistically significantly the UV-induced suppression of CHS to PCl. In the skin of mice fed with fruit and vegetables combined with UV irradiation the number of LC were comparable to the control mice, whereas the number of LC were significantly diminished in mice treated with UV only. This protective effect on the presence of LC in the epidermis after UV irradiation, which was also observed in a previous study with quercetin, may play a role in the prevention of UV-induced immunosuppression by the flavonoids tested. In conclusion, we found protection of flavonoids against UV-induced effects on CHS, which may be a common feature of most flavonoids.
authors.affiliation: Laboratory for Pathology and Immunobiology, National Institute of Public Health and the Environment, Bilthoven, The Netherlands. p.steerenberg@rivm.nl
authors.forename: P A
authors.initials: PA
authors.lastname: Steerenberg
authors.forename: J
authors.initials: J
authors.lastname: Garssen
authors.forename: P
authors.initials: P
authors.lastname: Dortant
authors.forename: P C
authors.initials: PC
authors.lastname: Hollman
authors.forename: G M
authors.initials: GM
authors.lastname: Alink
authors.forename: M
authors.initials: M
authors.lastname: Dekker
authors.forename: H B
authors.initials: HB
authors.lastname: Bueno-de-Mesquita
authors.forename: H
authors.initials: H
authors.lastname: Van Loveren
mesh_terms: D000821:Animal Feed; D000818:Animals; D003713:Dendritic Cells / Q000187:drug effects / Q000276:immunology / Q000528:radiation effects; D003877:Dermatitis, Contact / Q000517:prevention & control*; D005260:Female; D005419:Flavonoids / Q000494:pharmacology*; D005638:Fruit; D051379:Mice; D008812:Mice, Hairless; D010853:Picryl Chloride; D011794:Quercetin / Q000494:pharmacology*; D012867:Skin / Q000187:drug effects / Q000276:immunology / Q000528:radiation effects*; D013662:Tea; D014466:Ultraviolet Rays; D014675:Vegetables
chemical_list: D005419:Flavonoids; D013662:Tea; C043561:chrysin; D011794:Quercetin; D010853:Picryl Chloride