The Helicobacter felis ftsH gene encoding an ATP-dependent metalloprotease can replace the Escherichia coli homologue for growth and phage lambda lysogenization.
Cloning and sequencing of an approximately 6.0-kb chromosomal DNA fragment from Helicobacter felis revealed five complete open reading frames. The deduced amino acid sequence of one ORF exhibited sequence similarity to the FtsH protein, an ATP-dependent metalloprotease, from various bacterial species. The encoded protein consists of 638 amino acid residues with a molecular mass of 70.2 kDa. The hydropathy profile of the FtsH protein predicted two N-terminal transmembrane regions that were confirmed experimentally. Insertion of ftsH into a new versatile expression vector resulted in overexpression of FtsH protein in Escherichia coli. In addition, the E. coli ftsH gene could be replaced by the H. felis homologue to allow reduced growth and tenfold increased lysogenization by temperate phage lambda.
Melchers
K
K
Institute of Genetics, University of Bayreuth, D-95440 Bayreuth, Germany.
Wiegert
T
T
Buhmann
A
A
Postius
S
S
Schäfer
K P
KP
Schumann
W
W
D049069:ATP-Dependent Proteases; D001426:Bacterial Proteins / Q000737:chemistry / Q000235:genetics / Q000502:physiology*; D010582:Bacteriophage lambda / Q000254:growth & development*; D002462:Cell Membrane / Q000201:enzymology; D002874:Chromosome Mapping; D003001:Cloning, Molecular; D004926:Escherichia coli / Q000235:genetics / Q000254:growth & development* / Q000821:virology; D029968:Escherichia coli Proteins; D005798:Genes, Bacterial / Q000235:genetics; D016998:Helicobacter / Q000201:enzymology / Q000235:genetics*; D008242:Lysogeny / Q000235:genetics*; D008565:Membrane Proteins / Q000737:chemistry / Q000235:genetics / Q000502:physiology*; D008666:Metalloendopeptidases / Q000737:chemistry / Q000235:genetics / Q000502:physiology; D009876:Operon / Q000235:genetics; D011993:Recombinant Fusion Proteins; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D013696:Temperature
D001426:Bacterial Proteins; C071263:CopA protein, Bacteria; D029968:Escherichia coli Proteins; D008565:Membrane Proteins; D011993:Recombinant Fusion Proteins; D049069:ATP-Dependent Proteases; C513383:FtsH protein, E coli; D008666:Metalloendopeptidases