Scaffold attachment region-mediated enhancement of retroviral vector expression in primary T cells.
We have studied retroviral transgene expression in primary human lymphocytes. Our data demonstrate that transgene expression is high in activated primary CD4+ T cells but significantly decreased in mitotically quiescent cells. Incorporation of a DNA fragment from the scaffold attachment region (SAR) of the human beta interferon gene into the vector improved transgene expression, particularly in quiescent cells. The SAR element functioned in an orientation-dependent manner and enhanced expression of Moloney murine leukemia virus- and murine embryonic stem cell-based vectors. Clonal analysis of transduced T cells showed that the SAR sequence did not confer position-independent expression on a transgene but rather prevented the decrease of expression when cells became quiescent. The SAR sequence also enhanced transgene expression in T cells generated from retrovirally transduced CD34-enriched hematopoietic progenitor-stem cells in a SCID-hu thymus-liver mouse model. We have used the SAR-containing retroviral vector to express the RevM10 gene, a trans-dominant mutant of the human immunodeficiency virus type 1 (HIV-1) Rev gene. Compared to a standard retroviral vector, the SAR-containing vector was up to 2 orders of magnitude more efficient in inhibiting replication of the HIV-1 virus in infected CD4+ peripheral blood lymphocyte populations in vitro. This is the first demonstration that SAR elements can be used to improve retroviral vector expression in human primary T cells.
Agarwal
M
M
SyStemix, Inc., Palo Alto, California 94304, USA.
Austin
T W
TW
Morel
F
F
Chen
J
J
Böhnlein
E
E
Plavec
I
I
D016475:3T3 Cells; D000818:Animals; D000950:Antigens, Ly / Q000378:metabolism; D001665:Binding Sites; D015496:CD4-Positive T-Lymphocytes / Q000166:cytology / Q000821:virology*; D002472:Cell Transformation, Viral; D002478:Cells, Cultured; D002999:Clone Cells; D015870:Gene Expression; D015757:Genes, rev; D005822:Genetic Vectors; D015497:HIV-1 / Q000235:genetics / Q000502:physiology*; D006412:Hematopoietic Stem Cells / Q000378:metabolism; D006801:Humans; D016899:Interferon-beta / Q000235:genetics*; D016031:Liver Transplantation; D051379:Mice; D016513:Mice, SCID; D015530:Nuclear Matrix / Q000378:metabolism* / Q000821:virology; D012190:Retroviridae / Q000235:genetics*; D013950:Thymus Gland / Q000637:transplantation
D000950:Antigens, Ly; D016899:Interferon-beta