title: Rapid and progressive CD4+ decline in a monkey infected with an SIV+HIV-1 chimeric virus.
abstract: We previously constructed a simian immunodeficiency virus+human immunodeficiency virus type 1 (HIV-1) chimeric virus, NM-3rN to generate a pathogenic HIV-1 in macaque monkeys. During the in vivo passage of this virus in several monkeys, a viral strain, R43-56 was obtained which acquired a better replication ability in vivo. MM121, one of the three monkeys inoculated with the R43-56, showed weight loss, diarrhea and a rapid and continuous decrease in CD4+ lymphocytes at the moribund stage. An autopsy revealed generalized lymphadenopathy, dehydration, and ileocecal intussusception. In situ hybridization showed that the virus infection was in systemic lymphoid organs. We are presently monitoring the survivors to obtain candidates for a more virulent virus. R43-56 may be a better challenge virus and useful tool for human acquired immunodeficiency syndrome research.
authors.affiliation: Department of Pathology and Tumor Biology, Graduate School of Medicine, Kyoto, Japan.
authors.forename: D
authors.initials: D
authors.lastname: Nagamachi
authors.forename: T
authors.initials: T
authors.lastname: Igarashi
authors.forename: T
authors.initials: T
authors.lastname: Shimada
authors.forename: T
authors.initials: T
authors.lastname: Kuwata
authors.forename: M
authors.initials: M
authors.lastname: Ui
authors.forename: Y
authors.initials: Y
authors.lastname: Ami
authors.forename: Y
authors.initials: Y
authors.lastname: Enose
authors.forename: E
authors.initials: E
authors.lastname: Ido
authors.forename: M
authors.initials: M
authors.lastname: Fukumoto
authors.forename: M
authors.initials: M
authors.lastname: Hayami
mesh_terms: D000163:Acquired Immunodeficiency Syndrome / Q000276:immunology* / Q000503:physiopathology; D000818:Animals; D018791:CD4 Lymphocyte Count; D002678:Chimera; D003967:Diarrhea; D015497:HIV-1 / Q000235:genetics / Q000472:pathogenicity* / Q000502:physiology; D008253:Macaca mulatta; D012367:RNA, Viral / Q000032:analysis; D016097:Simian Acquired Immunodeficiency Syndrome / Q000276:immunology* / Q000503:physiopathology; D015302:Simian Immunodeficiency Virus / Q000235:genetics / Q000472:pathogenicity* / Q000502:physiology; D014779:Virus Replication; D015431:Weight Loss
chemical_list: D012367:RNA, Viral