Cloning of genes specifically expressed in rice embryogenic cells.
We have examined differences in gene expression pattern between embryogenic callus (EC) and nonembryogenic callus (NEC) derived from mature seed embryo of rice (Oryza sativa L. cv Donggin). Three EC-specific transcripts were identified by differential display of amplified cDNAs. Specific expression of two partial cDNAs, designated as REC1 and REC2, respectively, was confirmed by a northern blot analysis. Partial nucleotide sequence of the clone REC1 showed no homology with any known genes, but partial amino acid sequence deduced from the clone REC2 exhibited 55-82% homology with nickel-cobalt-resistant proteins identified from a bacterium, Alcaligenes eutrophus CH34.
Jung
B K
BK
Department of Medical Laboratory Technology, Seokang Junior College, Kwangju, Korea.
Pyo
J H
JH
Kim
W S
WS
Nam
B H
BH
Hwang
S J
SJ
Hwang
B
B
D000595:Amino Acid Sequence; D001483:Base Sequence; D003001:Cloning, Molecular; D018507:Gene Expression Regulation, Developmental; D018506:Gene Expression Regulation, Plant; D017343:Genes, Plant / Q000235:genetics*; D008855:Microscopy, Electron, Scanning; D008969:Molecular Sequence Data; D012275:Oryza / Q000196:embryology* / Q000235:genetics* / Q000648:ultrastructure; D016133:Polymerase Chain Reaction; D012639:Seeds / Q000196:embryology* / Q000235:genetics* / Q000648:ultrastructure