title: Lymphoid markers, activation markers, and adhesion molecules in cutaneous biopsy specimens from HIV+ patients with disease progression. The Military Medical Consortium for the Advancement of Retroviral Research.
abstract: BACKGROUND
  One important factor in understanding the pathogenesis of human immune deficiency virus (HIV) disease is documenting the patterns of immune dysregulation present in HIV-positive patients. The cells which home to skin are mainly certain subsets of T cells and, as opposed to the peripheral blood, where circulating factors may inhibit terminal phenotypic differentiation, the cutaneous environment potentiates differentiation during cutaneous eruptions.
  
  
  OBJECTIVE
  The authors' aim was to characterize the inflammatory dermatoses in biopsy specimens from HIV-positive patients with immunohistochemical stains for lymphoid markers, activation markers, and adhesion molecules and to determine if there was any correlation with the type of dermatosis and the HIV-disease stage.
  
  
  METHODS
  Lymphoid and activation markers as well as adhesion molecules were studied on cutaneous biopsy specimens from 96 inflammatory dermatoses in HIV-positive patients. The dermatoses included psoriasiform dermatoses with and without a lichenoid component, perivascular lymphoid dermatoses, perivascular and periadnexal inflammatory dermatoses, spongiotic dermatoses, granulomatous dermatoses, and neutrophilic dermatoses with and without vasculitis.
  
  
  RESULTS
  Although there was a decrease in CD4/CD8 ratios in the cutaneous inflammatory dermatoses with progression of the disease, the ratios of CD4/CD8 cells were far higher than those in the peripheral blood. There were also increasing numbers of CD23+ cells and increased E-Selectin expression on endothelial cells from the early stages of disease, with no consistent pattern of ICAM-1 expression on epithelial cells with disease progression.
  
  
  CONCLUSIONS
  The expression of lymphoid markers, activation markers, and adhesion molecules in the skin with progression of HIV disease, is consistent with a T helper (Th)1 to Th0/Th2 cytokine pattern of immune dysregulation. This cytokine pattern may be modified by the cytopathic effects of HIV on lymphoid and dendritic populations and by effects of other concurrent infections. Significant numbers of CD4+ T cells in skin infiltrates, with low peripheral CD4 T-cell counts, suggest that the cutaneous T-cell populations may be distinctive.
authors.affiliation: National Naval Medical Center, Bethesda, Maryland, USA.
authors.forename: K J
authors.initials: KJ
authors.lastname: Smith
authors.forename: H G
authors.initials: HG
authors.lastname: Skelton
authors.forename: J
authors.initials: J
authors.lastname: Yeager
authors.forename: D
authors.initials: D
authors.lastname: Baxter
authors.forename: A T
authors.initials: AT
authors.lastname: Nelson
authors.forename: P
authors.initials: P
authors.lastname: Angritt
authors.forename: W
authors.initials: W
authors.lastname: Chu
authors.forename: K F
authors.initials: KF
authors.lastname: Wagner
mesh_terms: D015703:Antigens, CD / Q000276:immunology; D015415:Biomarkers / Q000032:analysis*; D001706:Biopsy; D016516:CD4-CD8 Ratio; D016207:Cytokines / Q000276:immunology; D003872:Dermatitis / Q000150:complications / Q000276:immunology*; D018450:Disease Progression; D019040:E-Selectin / Q000032:analysis; D015658:HIV Infections / Q000150:complications / Q000276:immunology*; D006801:Humans; D007150:Immunohistochemistry; D018799:Intercellular Adhesion Molecule-1 / Q000032:analysis; D008212:Lymphocyte Depletion; D017455:Receptors, IgE / Q000032:analysis
chemical_list: D015703:Antigens, CD; D015415:Biomarkers; D016207:Cytokines; D019040:E-Selectin; D017455:Receptors, IgE; D018799:Intercellular Adhesion Molecule-1