Transcriptional regulation of fatty acid cyclooxygenases-1 and -2.
Transcriptional regulation of fatty acid cyclooxygenase isozymes (COX-1 and COX-2) were studied using two cell lines. A rapid and transient induction of COX-2 was observed by the addition of tumor necrosis factor alpha (TNF alpha) to murine osteoblastic cells MC3T3-E1. Possible involvement of NF kappa B and NF-IL6 as transcription factors was shown by deletion experiments using a luciferase reporter gene. COX-1 induction by a phorbol ester was demonstrated along with differentiation of human megakaryoblastic cells CMK to megakaryocytes.
Yamamoto
S
S
Department of Biochemistry, Tokushima University School of Medicine, Japan.
Yamamoto
K
K
Kurobe
H
H
Yamashita
R
R
Yamaguchi
H
H
Ueda
N
N
D000818:Animals; D051545:Cyclooxygenase 1; D051546:Cyclooxygenase 2; D005786:Gene Expression Regulation; D006801:Humans; D007527:Isoenzymes / Q000235:genetics*; D008565:Membrane Proteins; D051379:Mice; D011451:Prostaglandin-Endoperoxide Synthases / Q000235:genetics*; D014158:Transcription, Genetic
D007527:Isoenzymes; D008565:Membrane Proteins; D051545:Cyclooxygenase 1; D051546:Cyclooxygenase 2; C496511:PTGS1 protein, human; C496540:PTGS2 protein, human; D011451:Prostaglandin-Endoperoxide Synthases; C496512:Ptgs1 protein, mouse