title: Analytical development of electrospray and nanoelectrospray mass spectrometry in combination with liquid chromatography for the characterization of proteins.
abstract: Mass spectrometry has significantly extended its applicability in the area of characterization of protein structures. Electrospray ionization enables on-line coupling with liquid chromatography which has become a powerful tool for the characterization of peptide and protein mixtures. The most recent development of a nanoelectrospray source, using capillary forces for a particularly mild analyte transport and ionization into the mass spectrometer, opens a wide field for applications to protein structure analysis. In this paper, the analytical development of liquid chromatography-electrospray ionization mass spectrometry and nano-electrospray ionization mass spectrometry, adapted to an electrospray ionization quadrupole mass spectrometer and its application to the characterization of noncovalent protein complexes are described.
authors.affiliation: Fakultät für Chemie, Universität Konstanz, Germany.
authors.forename: T A
authors.initials: TA
authors.lastname: Fligge
authors.forename: K
authors.initials: K
authors.lastname: Bruns
authors.forename: M
authors.initials: M
authors.lastname: Przybylski
mesh_terms: D000595:Amino Acid Sequence; D000804:Angiotensin II / Q000737:chemistry; D000818:Animals; D002853:Chromatography, Liquid / Q000295:instrumentation / Q000379:methods*; D007987:Gonadotropin-Releasing Hormone / Q000737:chemistry; D013058:Mass Spectrometry / Q000295:instrumentation / Q000379:methods*; D008555:Melitten / Q000737:chemistry; D008969:Molecular Sequence Data; D008970:Molecular Weight; D009113:Muramidase / Q000032:analysis / Q000737:chemistry; D011506:Proteins / Q000032:analysis* / Q000737:chemistry
chemical_list: D011506:Proteins; D000804:Angiotensin II; D008555:Melitten; D007987:Gonadotropin-Releasing Hormone; D009113:Muramidase