title: Periodontal healing of extracted dogs' teeth air-dried for extended periods and soaked in various media.
abstract: The aim of this study was to determine the periodontal healing of replanted dogs' teeth which, after extended extra-oral dry times, had been soaked in various media before replantation. Incisors and premolars of beagle dogs were root canal treated, extracted and bench dried. The teeth were grouped according to dry times of 30, 45 and 60 minutes. Each group of teeth was soaked in one of three media, Hank's balanced salt solution, ViaSpan (Belzer UW-CSS, Dupont Pharmaceuticals), or Conditioned Medium (supernatant of confluent culture of human gingival fibroblasts) for 30 minutes before replantation. Controls consisted of teeth extracted and replanted without drying on soaking (negative control), and bench-dried teeth replanted without soaking in the media (positive control). The dogs were killed 6 months after replantation of the teeth, which were prepared for histologic evaluation. Five microns cross-sections (every 70 microns) of the root and surrounding tissue were evaluated for healing/resorption according to Andreasen's criteria. The best healing occurred for the roots which had been immediately replanted. Healing in the positive control groups decreased with increased dry time. For the 30-minute dry time groups, soaking in media had no beneficial effect on periodontal healing compared with the controls. Soaking in ViaSpan resulted in an increased healing incidence for both the 45- and 60-minute bench-dried groups while soaking in the other media had no consistent beneficial effect. It appears from this study that an avulsed tooth that has been left dry for 30 minutes should be replanted immediately without soaking. However, teeth that have been dry for 45 or 60 minutes would benefit from soaking for 30 minutes in ViaSpan.
authors.affiliation: Department of Endodontics, School of Dentistry, University of North Carolina, Chapel Hill 27599-7450, USA.
authors.forename: M
authors.initials: M
authors.lastname: Pettiette
authors.forename: J
authors.initials: J
authors.lastname: Hupp
authors.forename: S
authors.initials: S
authors.lastname: Mesaros
authors.forename: M
authors.initials: M
authors.lastname: Trope
mesh_terms: D000241:Adenosine; D000493:Allopurinol; D000704:Analysis of Variance; D000818:Animals; D001641:Bicuspid / Q000293:injuries; D016009:Chi-Square Distribution; D017077:Culture Media, Conditioned; D004285:Dogs; D005978:Glutathione; D006801:Humans; D007180:Incisor / Q000293:injuries; D007328:Insulin; D007552:Isotonic Solutions; D019188:Organ Preservation Solutions; D010519:Periodontium / Q000502:physiology*; D011887:Raffinose; D018709:Statistics, Nonparametric; D013997:Time Factors; D014021:Tissue Preservation / Q000379:methods*; D014084:Tooth Avulsion / Q000601:surgery; D014090:Tooth Replantation; D014945:Wound Healing
chemical_list: D017077:Culture Media, Conditioned; C072670:Hanks Balanced Salt Solution; D007328:Insulin; D007552:Isotonic Solutions; D019188:Organ Preservation Solutions; C055206:University of Wisconsin-lactobionate solution; D000493:Allopurinol; D005978:Glutathione; D000241:Adenosine; D011887:Raffinose