[Preparation of lymphotoxin and properties of it].
The process of isolation of highly purified human lymphotoxin was studied and optimized. A set of methods providing an increase in the content of the target product in the biomass (plasmid DNA amplification and selection of clones of transformed cells) was applied at the stage of cultivation. A two-step purification scheme (ion-exchange chromatography on DEAE-Biotone A1 and hydroxylapatite) was developed. A number of physical characteristics were studied.
Lebedev
L R
LR
Research and Technology Institute of Biologically Active Substances, Berdsk, Novosibirsk oblast', Russia.
Pustoshilova
N M
NM
Sinichkina
S A
SA
Andreeva
I S
IS
D002852:Chromatography, Ion Exchange; D003001:Cloning, Molecular; D004591:Electrophoresis, Polyacrylamide Gel; D004926:Escherichia coli / Q000235:genetics; D006801:Humans; D008233:Lymphotoxin-alpha / Q000235:genetics* / Q000302:isolation & purification / Q000378:metabolism; D011994:Recombinant Proteins / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism
D008233:Lymphotoxin-alpha; D011994:Recombinant Proteins