title: Increased beta-actin expression in an invasive moloney sarcoma virus-transformed MDCK cell variant concentrates to the tips of multiple pseudopodia.
abstract: An invasive variant of Moloney sarcoma virus-transformed MDCK cells (MSV-MDCK-INV), which was isolated by the repeated selection of cells that successfully traversed a Matrigel-coated filter, exhibits increased motile ability and presents an elongated cell shape and numerous pseudopodia. Although stress fibers are present in both MDCK and MSV-MDCK cells, MSV-MDCK-INV cells contain no stress fibers and exhibit a dense concentration of actin at the tips of pseudopodia. Relative to both MDCK and MSV-MDCK cells, the MSV-MDCK-INV cells exhibit increased expression of beta-actin and redistribution of beta-actin to the tips of pseudopodia. These actin concentrations are enriched in both F- and G-actin and, thus, represent dynamic regions of actin cytoskeleton remodeling. The acquisition of invasive properties by epithelial transformants is, therefore, associated with the increased expression of beta-actin and its concentration in actin-rich domains, which may drive pseudopodial extension and facilitate tumor cell invasion.
authors.affiliation: Département de Pathologie et Biologie Cellulaire, Université de Montréal, Québec, Canada.
authors.forename: P U
authors.initials: PU
authors.lastname: Le
authors.forename: T N
authors.initials: TN
authors.lastname: Nguyen
authors.forename: P
authors.initials: P
authors.lastname: Drolet-Savoie
authors.forename: N
authors.initials: N
authors.lastname: Leclerc
authors.forename: I R
authors.initials: IR
authors.lastname: Nabi
mesh_terms: D000199:Actins / Q000378:metabolism* / Q000502:physiology; D000818:Animals; D002461:Cell Line, Transformed; D048429:Cell Size; D002472:Cell Transformation, Viral; D004285:Dogs; D015151:Immunoblotting; D007668:Kidney; D008856:Microscopy, Fluorescence; D008980:Moloney murine sarcoma virus; D009361:Neoplasm Invasiveness; D011554:Pseudopodia / Q000378:metabolism*
chemical_list: D000199:Actins