Pulsed-field gel electrophoresis typing of Hafnia alvei isolated from chub-packed and retail ground beef.
Thirty-eight isolates of Hafnia alvei were characterized by biochemical profiles, ribotyping and pulsed-field gel electrophoresis (PFGE) patterns. The isolates were recovered from chub-packed (19 isolates) or retail (nine isolates) ground beef, or were obtained from culture repositories (10 isolates). Biochemical profiling differentiated the 38 isolates into five groups and a commercial ribotyping method recognized 11 groups, whereas PFGE differentiated the same 38 isolates into 19 groups. These data substantiate that PFGE is a highly discriminatory tool for establishing the relatedness among Hafnia alvei strains.
Gamage
S D
SD
University of Wisconsin, Department of Food Science, Madison 53706, USA.
Luchansky
J B
JB
Ingham
S C
SC
D000818:Animals; D015373:Bacterial Typing Techniques; D002417:Cattle; D016521:Electrophoresis, Gel, Pulsed-Field; D004755:Enterobacteriaceae / Q000145:classification*; D008460:Meat / Q000382:microbiology*