title: Diffusion, perfusion, and T2 magnetic resonance imaging of anti-intercellular adhesion molecule 1 antibody treatment of transient middle cerebral artery occlusion in rat.
abstract: The effect of anti-intercellular adhesion molecule-1 (anti-ICAM-1) antibody treatment of transient (2 h) middle cerebral artery (MCA) occlusion in the rat was measured using diffusion (DWI)-, T2 (T2I)- and perfusion (PWI)-weighted magnetic resonance imaging. Rats were treated upon reperfusion with an anti-ICAM-1 monoclonal antibody (n=11) or a control antibody (n=7). DWI, T2I and PWI were performed before, during, and after induction of focal cerebral ischemia from 1 h to 7 days. In both groups, the apparent diffusion coefficient of water (ADCw) and cerebral blood flow (CBF) values in the ischemic region significantly declined from the preischemic ADCw values (p<0. 05). The post ischemic increase in T2 of the control group was significantly higher at 48 h than in the anti-ICAM-1 treated group (p<0.05). CBF was not significantly different between the two groups. The temporal profiles of MRI cluster analysis, which combines ADCw and T2 maps into a single image, was significantly different between groups. These data suggest that the neuroprotective effect of anti-ICAM-1 antibody treatment is reflected in reductions of T2 and lesion growth during reperfusion and may not be associated with increased cerebral perfusion.
authors.affiliation: Henry Ford Health Sciences Center, Department of Neurology, Detroit, MI 48202, USA.
authors.forename: Q
authors.initials: Q
authors.lastname: Jiang
authors.forename: Z G
authors.initials: ZG
authors.lastname: Zhang
authors.forename: R L
authors.initials: RL
authors.lastname: Zhang
authors.forename: J R
authors.initials: JR
authors.lastname: Ewing
authors.forename: G W
authors.initials: GW
authors.lastname: Divine
authors.forename: P
authors.initials: P
authors.lastname: Jiang
authors.forename: M
authors.initials: M
authors.lastname: Chopp
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal; D001157:Arterial Occlusive Diseases / Q000175:diagnosis* / Q000378:metabolism; D001931:Brain Mapping; D002536:Cerebral Arteries / Q000378:metabolism / Q000473:pathology; D002560:Cerebrovascular Circulation / Q000502:physiology*; D004058:Diffusion; D018799:Intercellular Adhesion Molecule-1 / Q000276:immunology*; D002546:Ischemic Attack, Transient / Q000175:diagnosis* / Q000378:metabolism; D008279:Magnetic Resonance Imaging / Q000379:methods*; D008297:Male; D010477:Perfusion; D051381:Rats; D017208:Rats, Wistar
chemical_list: D000911:Antibodies, Monoclonal; D018799:Intercellular Adhesion Molecule-1