Prostaglandin and progesterone production by bovine luteal cells incubated in the presence or absence of the accessory cells of the corpus luteum and treated with interleukin-1beta, indomethacin and luteinizing hormone.
This experiment examined production of prostaglandin (PG) F2alpha, PGE2 and progesterone by bovine luteal cells incubated with or without the accessory cells and treated with recombinant bovine interleukin-1beta (10 ng), indomethacin (5 microg) or luteinizing hormone (50 ng). During pretreatment, progesterone production was similar in the luteal and luteal plus accessory cells, being greater in either of these than in accessory cells; PGF2alpha was greatest in luteal plus accessory, intermediate in accessory and lowest in the luteal cells; PGE2 was similar among all cell arrangements. Luteinizing hormone or luteinizing hormone plus indomethacin stimulated progesterone in the luteal and luteal plus accessory cells, this being similar in these two cell arrangements. Interleukin-1beta stimulated PGF2alpha in luteal and luteal plus accessory cells, and tended to elevate PGF2alpha in accessory cells. Interleukin-1beta stimulated PGE2 in all cell arrangements. Indomethacin inhibited the interleukin-1beta-stimulated PGF2alpha and PGE2 release. Indomethacin or indomethacin plus luteinizing hormone inhibited basal PGE2 in luteal and luteal plus accessory cells. These data suggest that progesterone production is not influenced by the presence of accessory cells of the corpus luteum, that accessory and luteal cells produce appreciable amounts of PG, and that recombinant bovine interleukin-1beta stimulates PGE2 and PGF2alpha in the luteal and accessory cells.
Del Vecchio
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RP
Agriculture & Agri-Food Canada Research Centre, Brandon, Manitoba.
Sutherland
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WD
D000818:Animals; D002417:Cattle / Q000378:metabolism*; D003338:Corpus Luteum / Q000166:cytology / Q000502:physiology; D015237:Dinoprost / Q000096:biosynthesis; D015232:Dinoprostone / Q000096:biosynthesis; D005260:Female; D007213:Indomethacin / Q000494:pharmacology*; D007375:Interleukin-1 / Q000494:pharmacology*; D008184:Luteal Cells / Q000187:drug effects / Q000378:metabolism*; D007986:Luteinizing Hormone / Q000494:pharmacology*; D011374:Progesterone / Q000096:biosynthesis*; D011453:Prostaglandins / Q000096:biosynthesis*
D007375:Interleukin-1; D011453:Prostaglandins; D011374:Progesterone; D007986:Luteinizing Hormone; D015237:Dinoprost; D015232:Dinoprostone; D007213:Indomethacin