Paramagnetic Inversion of the Sign of the Interference Contribution to the Transverse Relaxation of the Imido Protons of the Coordinated Imidazoles in the Uniformly 15N-Labeled Cytochrome c3
In the spectrum of uniformly 15N-labeled cytochrome c3, the relative linewidths of the doublet peaks of the 15N-coupled imido proton of the coordinated imidazole group were reversed on oxidation. This inversion was explained by the interference relaxation process between the electron-proton dipolar and 15N-1H dipolar interactions. The inversion can be used to assign the imido protons of the coordinated imidazole groups in heme proteins. Copyright 1998 Academic Press.
Ohmura
T
Faculty of Engineering, Yokohama National University, Hodogaya-ku, Yokohama, 240, Japan
Harada
E
Fujiwara
T
Kawai
G
Watanabe
K
Akutsu
H