title: Mutations in aarE, the ubiA homolog of Providencia stuartii, result in high-level aminoglycoside resistance and reduced expression of the chromosomal aminoglycoside 2'-N-acetyltransferase.
abstract: The aarE1 allele was identified on the basis of the resulting phenotype of increased aminoglycoside resistance. The aarE1 mutation also resulted in a small-colony phenotype and decreased levels of aac(2')-Ia mRNA. The deduced AarE gene product displayed 61% amino acid identity to the Escherichia coli UbiA protein, an octaprenyltransferase required for the second step of ubiquinone biosynthesis. Complementation experiments in both Providencia stuartii and E. coli demonstrated that aarE and ubiA are functionally equivalent.
authors.affiliation: Department of Medicine, Case Western Reserve University School of Medicine, Cleveland, Ohio 44106, USA.
authors.forename: M R
authors.initials: MR
authors.lastname: Paradise
authors.forename: G
authors.initials: G
authors.lastname: Cook
authors.forename: R K
authors.initials: RK
authors.lastname: Poole
authors.forename: P N
authors.initials: PN
authors.lastname: Rather
mesh_terms: D000123:Acetyltransferases / Q000235:genetics / Q000378:metabolism*; D019883:Alkyl and Aryl Transferases / Q000235:genetics / Q000378:metabolism; D000483:Alleles; D000617:Aminoglycosides; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D015152:Blotting, Northern; D002876:Chromosomes, Bacterial / Q000187:drug effects; D004269:DNA, Bacterial / Q000737:chemistry; D008969:Molecular Sequence Data; D010957:Plasmids; D011532:Providencia / Q000187:drug effects / Q000201:enzymology / Q000235:genetics*
chemical_list: D000617:Aminoglycosides; D000900:Anti-Bacterial Agents; D004269:DNA, Bacterial; D000123:Acetyltransferases; C019681:aminoglycoside acetyltransferase; D019883:Alkyl and Aryl Transferases; C021386:4-hydroxybenzoate polyprenyltransferase