title: Transcription of multiple cell wall protein-encoding genes in Saccharomyces cerevisiae is differentially regulated during the cell cycle.
abstract: The yeast cell wall consists of an internal skeletal layer and an outside protein layer. The synthesis of both beta-1,3-glucan and chitin, which together from the cell wall skeleton, is cell cycle-regulated. We show here that the expression of five cell wall protein-encoding genes (CWP1, CWP2, SED1, TIP1 and TIR1) is also cell cycle-regulated. TIP1 is expressed in G1 phase, CWP1, CWP2 and TIR1 are expressed in S/G2 phase, and SED1 in M phase. The data suggest that these proteins fulfil distinct functions in the cell wall.
authors.affiliation: Institute for Molecular Cell Biology, BioCentrum Amsterdam, University of Amsterdam, The Netherlands.
authors.forename: L H
authors.initials: LH
authors.lastname: Caro
authors.forename: G J
authors.initials: GJ
authors.lastname: Smits
authors.forename: P
authors.initials: P
authors.lastname: van Egmond
authors.forename: J W
authors.initials: JW
authors.lastname: Chapman
authors.forename: F M
authors.initials: FM
authors.lastname: Klis
mesh_terms: D015152:Blotting, Northern; D002453:Cell Cycle / Q000235:genetics; D002473:Cell Wall / Q000737:chemistry / Q000235:genetics; D005656:Fungal Proteins / Q000235:genetics* / Q000378:metabolism; D005800:Genes, Fungal / Q000235:genetics*; D008565:Membrane Proteins / Q000235:genetics* / Q000378:metabolism; D010641:Phenotype; D012333:RNA, Messenger / Q000302:isolation & purification; D012441:Saccharomyces cerevisiae / Q000737:chemistry / Q000235:genetics*; D014158:Transcription, Genetic / Q000235:genetics*
chemical_list: D005656:Fungal Proteins; D008565:Membrane Proteins; D012333:RNA, Messenger