Characterization of Fv fragments expressed on phage surface.
We characterized a phage antibody in which an Fv fragment, namely, a free VH fragment noncovalently associated with a VL fragment that is fused with a truncated cpIII molecule (VL-DeltacpIII), is expressed on the phage surface. D1.3 antibody specific for hen egg-white lysozyme was used as a model system. Both VH and VL-DeltacpIII fragments were stably expressed and associated with each other to form a faithful antigen-binding site. The results of Western blotting indicated that more than 5% of phages expressed the Fv fragment on their surface. Analysis of the kinetics of binding of the phage antibody to the antigen suggested the possibility of presence of phages having multiple-binding sites on a single phage particle.
Ito
W
W
Institute for Comprehensive Medical Science, Fujita Health University, Toyoake, Aichi 470-1192, Japan.
Kurosawa
Y
Y
D000818:Animals; D000914:Antibodies, Viral / Q000276:immunology; D000937:Antigen-Antibody Reactions; D017104:Bacteriophage M13 / Q000276:immunology / Q000378:metabolism*; D001666:Binding Sites, Antibody; D015374:Biosensing Techniques; D002645:Chickens; D002851:Chromatography, High Pressure Liquid; D003902:Detergents; D004529:Egg White; D007128:Immunoglobulin Fragments / Q000096:biosynthesis / Q000737:chemistry* / Q000235:genetics / Q000276:immunology; D009113:Muramidase / Q000276:immunology; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis / Q000737:chemistry / Q000235:genetics / Q000276:immunology; D012521:Sarcosine / Q000031:analogs & derivatives; D013205:Staphylococcal Protein A / Q000096:biosynthesis / Q000737:chemistry / Q000235:genetics / Q000276:immunology
D000914:Antibodies, Viral; D003902:Detergents; D007128:Immunoglobulin Fragments; D011993:Recombinant Fusion Proteins; D013205:Staphylococcal Protein A; C055966:immunoglobulin Fv; C025231:sarkosyl; D009113:Muramidase; D012521:Sarcosine