Isolation and differential secretion of metalloproteinase by superficial chondrocytes in articular cartilage.
Chondrocytes from superficial layers of articular cartilage have distinct phenotypic properties which are different from those of cells obtained from the deeper areas. We describe a method that isolates highly purified articular cartilage chondrocytes from the superficial layers. When the superficial cells are stimulated in vitro with a source of cytokines, they secrete greater amounts of metalloproteinase compared to chondrocytes obtained from a deeper area.
Takagi
T
T
Division of Rheumatology, University of Arkansas for Medical Sciences, Veterans Administration Hospital, Little Rock, USA.
Takahashi
S
S
Koshino
T
T
Okamoto
R
R
Jasin
H E
HE
D000818:Animals; D002358:Cartilage, Articular / Q000378:metabolism*; D002417:Cattle; D019902:Chondrocytes / Q000378:metabolism*; D005434:Flow Cytometry; D008666:Metalloendopeptidases / Q000378:metabolism*
D008666:Metalloendopeptidases