title: Staining pattern of seven monoclonal anti-CD26 antibodies in leprosy: implications for the use of CD26 as a surrogate marker of a human Th1-like reaction.
abstract: In a previous study using the monoclonal anti-CD26 antibody MIB-DS2/7 in leprosy and other granulomatous diseases, it was shown that CD26 may be a candidate for use as an operational marker of a human Th1-like reaction. In this follow-up study, we compared seven different monoclonal anti-CD26 antibodies with respect to their staining pattern in lepromatous and tuberculoid leprosy tissues. Three distinct staining patterns became apparent in this anti-CD26 antibody panel: staining of T-lymphocytes and of connective tissue; staining of T-lymphocytes, connective tissue and macrophages; and almost no staining of T-lymphocytes but staining of connective tissue and macrophages. The two antibodies assigned to the first staining pattern, including MIB-DS2/7, were found to be most suitable for the operational discrimination between Th1-like and Th2-like reactions in leprosy. The antibodies assigned to staining patterns 2 and 3 did not allow this discrimination. Although all seven monoclonal antibodies investigated were specific for CD26, only two were found to be useful in identifying a Th1-like immune reaction in human tissue.
authors.affiliation: Department of Immunology and Cell Biology, Forschungszentrum Borstel, Germany.
authors.forename: U
authors.initials: U
authors.lastname: Seitzer
authors.forename: D
authors.initials: D
authors.lastname: Scheel-Toellner
authors.forename: T
authors.initials: T
authors.lastname: Mattern
authors.forename: H
authors.initials: H
authors.lastname: Haas
authors.forename: H D
authors.initials: HD
authors.lastname: Flad
authors.forename: J
authors.initials: J
authors.lastname: Gerdes
mesh_terms: D000911:Antibodies, Monoclonal / Q000276:immunology*; D015415:Biomarkers; D003238:Connective Tissue / Q000276:immunology; D018819:Dipeptidyl Peptidase 4 / Q000276:immunology*; D006801:Humans; D007150:Immunohistochemistry; D007918:Leprosy / Q000276:immunology*; D008264:Macrophages / Q000276:immunology; D013601:T-Lymphocytes / Q000276:immunology; D018417:Th1 Cells / Q000276:immunology; D018418:Th2 Cells / Q000276:immunology
chemical_list: D000911:Antibodies, Monoclonal; D015415:Biomarkers; D018819:Dipeptidyl Peptidase 4