A fluorescence method for estimation of toxemia: binding capacity of lipoproteins and albumin in plasma.
The hazard of toxemia, a condition resulting from the spread of toxins by the bloodstream, is regulated by plasma proteins capable of binding with free toxins. As toxin binding results in a reduction of available binding sites, measuring the proteins' binding capacity can be used to estimate toxemia severity. Suggested by this approach, a novel fluorescence method was developed to determine lipoprotein and albumin binding capacities in whole plasma. The method entails two steps: specific binding of N(n-carboxy)phenylimide-4-dimethyl-aminonaphthalic acid with albumin followed by addition of 12-(9-anthroyloxy)stearic acid which, under these conditions, binds mostly with lipoprotein. Reduced fluorescence intensity of the probes in plasma of patients compared to that of healthy donors reflected saturation of binding sites by toxins, thereby estimating toxemia severity. Poor correlation was found between the lipoprotein and albumin binding abilities, suggesting their independent diagnostic values. The simplicity and rapidity of this method are advantageous for its clinical application.
Altamentova
S M
SM
Sackler Institute of Molecular Medicine, Sackler Faculty of Medicine, Tel Aviv University, Israel.
Shaklai
N
N
Arav
R
R
Miller
Y I
YI
D000328:Adult; D005260:Female; D005456:Fluorescent Dyes / Q000032:analysis / Q000378:metabolism*; D006801:Humans; D007094:Imides / Q000032:analysis; D008074:Lipoproteins / Q000097:blood*; D008297:Male; D008875:Middle Aged; D009281:Naphthalenes / Q000032:analysis; D011485:Protein Binding; D012709:Serum Albumin / Q000378:metabolism*; D013050:Spectrometry, Fluorescence; D013229:Stearic Acids / Q000097:blood; D014115:Toxemia / Q000097:blood*
D005456:Fluorescent Dyes; D007094:Imides; D008074:Lipoproteins; C112259:N(n-carboxy)phenylimide-4-dimethyl-aminonaphthalic acid; D009281:Naphthalenes; D012709:Serum Albumin; D013229:Stearic Acids; C026653:12-(9-anthroyloxy)stearic acid