Arachidonic acid and human bone marrow stromal cells.
Human bone marrow stromal cells regulate the growth of marrow hematopoietic progenitors by secreting cytokines. Arachidonic acid (AA) is the fatty acid precursor of prostaglandin E2 (PGE2) and leukotriene B4 (LTB4) that modulate the growth of human bone marrow progenitors. We have investigated the incorporation of AA in human bone marrow stromal cell cultures, their production of PGE2 and LTB4 and the effect of AA on their growth. Gas chromatography analysis reveals the presence of AA in the human bone marrow plasma and in bone marrow stromal cell cultures. In stromal cells, [3H]-AA is incorporated into triglycerides and is later delivered into phospholipids. Prelabeling-chase experiments indicate a preferential incorporation of AA into phosphatidylethanolamine and no trafficking of labeled AA between phospholipid species. Bone marrow stromal cells release PGE2 and LTB4 in response to phorbol myristic acetate (PMA) (1 microM) and tumor necrosis factor alpha (TNF-alpha) (10 ng/ml). Exogenous AA (up to 1 microM) has no significant effect on cell growth. In conclusion, human bone marrow stromal cells capt exogenous AA and, thus, may participate to the control of marrow AA concentrations. They may also regulate human marrow hematopoiesis by secreting AA metabolites such as PGE2 and LTB4.
Denizot
Y
Y
Laboratoire d'Hématologie Expérimentale, Faculté de Médecine, Limoges, France.
Dulery
C
C
Trimoreau
F
F
Desplat
V
V
Praloran
V
V
D015121:6-Ketoprostaglandin F1 alpha / Q000378:metabolism; D016718:Arachidonic Acid / Q000378:metabolism* / Q000493:pharmacokinetics; D001853:Bone Marrow / Q000737:chemistry / Q000378:metabolism*; D001854:Bone Marrow Cells / Q000187:drug effects / Q000378:metabolism*; D002455:Cell Division / Q000187:drug effects; D002478:Cells, Cultured; D015232:Dinoprostone / Q000378:metabolism; D005227:Fatty Acids / Q000032:analysis; D006801:Humans; D015850:Interleukin-6 / Q000494:pharmacology; D007975:Leukotriene B4 / Q000378:metabolism; D050356:Lipid Metabolism; D010743:Phospholipids / Q000378:metabolism; D017154:Stromal Cells / Q000737:chemistry / Q000187:drug effects / Q000378:metabolism; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology; D014316:Tritium; D014409:Tumor Necrosis Factor-alpha / Q000494:pharmacology
D005227:Fatty Acids; D015850:Interleukin-6; D010743:Phospholipids; D014409:Tumor Necrosis Factor-alpha; D014316:Tritium; D007975:Leukotriene B4; D016718:Arachidonic Acid; D015121:6-Ketoprostaglandin F1 alpha; D015232:Dinoprostone; D013755:Tetradecanoylphorbol Acetate