title: Characterization of canine pericardial fluid endothelin-1 levels.
abstract: Recently, extremely high concentrations of endothelin-1 (ET-1) were detected in the pericardial fluid of patients undergoing cardiac surgery. This study was designed to characterize ET-1 levels in plasma and pericardial fluid of dogs. Plasma and pericardial fluid samples were extracted using SepPak C18 cartridges and ET-1 levels were measured by a specific radioimmunoassay. Pericardial fluid ET-1 levels (739 +/- 81 pg/ml; n = 10) were significantly higher than respective plasma levels (22.4 +/- 4.0 pg/ml; p < 0.05). The mean pericardial fluid: plasma ratio of ir-ET-1 concentration was 54.7 +/- 16.8. In HPLC analysis, the total ET-1-like immunoreactivity of pericardial fluid co-eluted with ET-1 standard. In anesthetized dogs (n = 6), disappearance of [125I]ET-1 was sixfold slower in the pericardial space than in plasma. Our results show that high concentrations of ir-ET-1 can be found in canine pericardial fluid. Slow elimination of ET-1 from the pericardial fluid compartment may contribute to high peptide levels.
authors.affiliation: Department of Cardiovascular Surgery, Semmelweis University Medical School, Budapest, Hungary.
authors.forename: I
authors.initials: I
authors.lastname: Szokodi
authors.forename: F
authors.initials: F
authors.lastname: Horkay
authors.forename: P
authors.initials: P
authors.lastname: Kiss
authors.forename: L
authors.initials: L
authors.lastname: Selmeci
authors.forename: I
authors.initials: I
authors.lastname: Horváth
authors.forename: O
authors.initials: O
authors.lastname: Vuolteenaho
authors.forename: H
authors.initials: H
authors.lastname: Ruskoaho
authors.forename: A
authors.initials: A
authors.lastname: Juhász-Nagy
authors.forename: M
authors.initials: M
authors.lastname: Tóth
mesh_terms: D000818:Animals; D001826:Body Fluids / Q000378:metabolism; D002851:Chromatography, High Pressure Liquid; D004285:Dogs; D019332:Endothelin-1 / Q000097:blood / Q000378:metabolism*; D006207:Half-Life; D007457:Iodine Radioisotopes; D010496:Pericardium / Q000378:metabolism*; D011863:Radioimmunoassay
chemical_list: D019332:Endothelin-1; D007457:Iodine Radioisotopes