The profile of the cytokines secreted during the generation of T-helper cells from atopic asthmatic subjects.
This study investigated cytokine release by T-cell lines from atopic and nonatopic individuals in the presence of specific aeroallergen. Cell lines from atopic and nonatopic individuals secreted IL-2 for less than 14 and more than 21 days, respectively. All of the atopic, but not the nonatopic, cell lines exhibited a biphasic peak in IL-4 and IL-5 secretion. Flow cytometry revealed that, after 35 days, 89.3% of the atopic cells were T helpers and 73.2% were activated. Only 7.4% of the nonatopic cells displayed activation markers. In conclusion, T-cell differentiation may be controlled by other factors in addition to stimulation by aeroallergens.
Crocker
I C
IC
Department of Pharmaceutical Sciences, Creighton University Health Sciences Center, Omaha, Nebraska 68178, USA.
Gupta
K
K
Townley
R G
RG
Khan
M M
MM
D000328:Adult; D000485:Allergens; D001249:Asthma / Q000276:immunology*; D002460:Cell Line; D005434:Flow Cytometry; D006801:Humans; D006969:Hypersensitivity, Immediate / Q000276:immunology*; D007376:Interleukin-2 / Q000378:metabolism*; D015847:Interleukin-4 / Q000378:metabolism*; D015848:Interleukin-5 / Q000378:metabolism*; D006377:T-Lymphocytes, Helper-Inducer / Q000145:classification / Q000378:metabolism*; D013997:Time Factors
D000485:Allergens; D007376:Interleukin-2; D015848:Interleukin-5; D015847:Interleukin-4