title: Effect of alignment of the transplanted graft extracellular matrix on cellular repopulation and newly synthesized collagen.
abstract: This study examines the effect of alignment of the transplanted graft extracellular matrix on cellular repopulation and new collagen synthesis. The lateral half of the patellar tendon was harvested as a tendon graft from Lewis rats and frozen at -80 degrees C. In order to maintain the original alignment of the graft extracellular matrix, the graft was transplanted to a same size defect in the patellar tendon of other Lewis rats (group I). For controls, the graft was transplanted in a lax condition after excision of only the distal half of the lateral side of a patellar tendon (group II). After transplantation, six animals in each group were killed at 3, 7, 14, and 28 days. Cellular repopulation was assessed by using fibrillar-actin (F-actin) labeling with rhodamine-phalloidin, and new collagen synthesis was detected by means of a polyclonal antibody against type III collagen aminopropeptide (pN collagen III). Collagen fibril profiles were observed under the transmission electron microscope. On the 3rd day after transplantation, no specific fluorescence was detected in either group. Specific labeling for F-actin and pN collagen III, however, was observed at both ends of the graft in both groups at 1 week and throughout the graft at 2 weeks after transplantation. Consistent with the actin bundles' orientation, pN collagen III was aligned parallel to the longitudinal axis of the graft in group I. Collagen fibrils with a smaller diameter, mixed evenly and everywhere with a larger diameter, increased gradually in group I. However, smaller collagen fibrils in group II increased more slowly and were distributed unevenly. In summary, the dense collagen arrangement in the native patellar tendon determined the alignment of the repopulating cells, and the distribution of newly synthesized collagen might be affected by the actin cytoskeleton within the repopulating cells.
authors.affiliation: Department of Orthopaedic Surgery, Osaka University Medical School, Japan.
authors.forename: N
authors.initials: N
authors.lastname: Matsumoto
authors.forename: S
authors.initials: S
authors.lastname: Horibe
authors.forename: N
authors.initials: N
authors.lastname: Nakamura
authors.forename: T
authors.initials: T
authors.lastname: Senda
authors.forename: K
authors.initials: K
authors.lastname: Shino
authors.forename: T
authors.initials: T
authors.lastname: Ochi
mesh_terms: D000199:Actins / Q000032:analysis; D000818:Animals; D002455:Cell Division / Q000502:physiology*; D003094:Collagen / Q000096:biosynthesis* / Q000648:ultrastructure; D003599:Cytoskeleton / Q000648:ultrastructure; D005109:Extracellular Matrix / Q000473:pathology*; D006085:Graft Survival / Q000502:physiology*; D008297:Male; D008854:Microscopy, Electron; D008856:Microscopy, Fluorescence; D017847:Patellar Ligament / Q000473:pathology / Q000637:transplantation; D051381:Rats; D011917:Rats, Inbred Lew; D013709:Tendon Transfer / Q000379:methods*
chemical_list: D000199:Actins; D003094:Collagen