In situ hybridization with riboprobes: an overview for veterinary pathologists.
In situ hybridization using nonradioactively-labeled RNA probes is a technique that combines understanding of basic molecular biology and histopathologic interpretation. Recombinant or PCR technology can be used to produce probes that hybridize with a wide variety of cellular genes and infectious agents. Hybridization conditions can be optimized for each probe/target combination.
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Department of Veterinary Pathology, College of Veterinary Medicine, University of Georgia, Athens 30602-7388, USA.
D000818:Animals; D017403:In Situ Hybridization / Q000379:methods / Q000662:veterinary*; D010341:Pathology, Veterinary / Q000379:methods*; D012313:RNA / Q000737:chemistry; D015347:RNA Probes
D015347:RNA Probes; D012313:RNA