Identification and enzymatic characterization of the maltose-inducible alpha-glucosidase MalL (sucrase-isomaltase-maltase) of Bacillus subtilis.
A gene coding for a putative alpha-glucosidase has been identified in the open reading frame yvdL (now termed malL), which was sequenced as part of the Bacillus subtilis genome project. The enzyme was overproduced in Escherichia coli and purified. Further analyses indicate that MalL is a specific oligo-1,4-1,6-alpha-glucosidase (sucrase-maltase-isomaltase). MalL expression in B. subtilis requires maltose induction and is subject to carbon catabolite repression by glucose and fructose. Insertional mutagenesis of malL resulted in a complete inactivation of the maltose-inducible alpha-glucosidase activity in crude protein extracts and a Mal- phenotype.
Schönert
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Lehrstuhl für Mikrobiologie, Institut für Mikrobiologie, Biochemie und Genetik, Universität Erlangen-Nürnberg, Erlangen, Germany.
Buder
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Dahl
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MK
D001412:Bacillus subtilis / Q000187:drug effects / Q000201:enzymology / Q000235:genetics*; D001426:Bacterial Proteins / Q000096:biosynthesis / Q000235:genetics; D003001:Cloning, Molecular; D004790:Enzyme Induction; D004794:Enzyme Repression; D004926:Escherichia coli / Q000235:genetics; D005632:Fructose / Q000494:pharmacology; D005947:Glucose / Q000494:pharmacology; D008320:Maltose / Q000494:pharmacology; D016254:Mutagenesis, Insertional; D016366:Open Reading Frames; D010641:Phenotype; D011994:Recombinant Proteins / Q000096:biosynthesis; D013394:Sucrase-Isomaltase Complex / Q000235:genetics*; D000520:alpha-Glucosidases / Q000235:genetics*
D001426:Bacterial Proteins; D011994:Recombinant Proteins; D005632:Fructose; D008320:Maltose; D013394:Sucrase-Isomaltase Complex; C112179:sucrase-isomaltase-maltase; D000520:alpha-Glucosidases; D005947:Glucose