title: The leuX-encoded tRNA5(Leu) but not the pathogenicity islands I and II influence the survival of the uropathogenic Escherichia coli strain 536 in CD-1 mouse bladder mucus in the stationary phase.
abstract: The uropathogenic Escherichia coli strain 536 carries two pathogenicity islands, each of which is associated with either of the tRNA genes selC or leuX, respectively. Growth competition in CD-1 mouse mucus between the wild-type strain E. coli 536, its leuX mutant 536 delta 102 and its mutant 536R3, lacking both pathogenicity islands but expressing a functional tRNA5(Leu), revealed a major impact of leuX on E. coli survival in bladder mucus. The impaired survival in CD-1 mouse mucus observed upon deletion of the leuX gene was abolished after complementation with the leuX gene. The survival of bacteria in bladder mucus was not influenced by the presence of pathogenicity islands I and II.
authors.affiliation: Institut für Molekulare Infektionsbiologie, Universität Würzburg, Germany.
authors.forename: U
authors.initials: U
authors.lastname: Dobrindt
authors.forename: P S
authors.initials: PS
authors.lastname: Cohen
authors.forename: M
authors.initials: M
authors.lastname: Utley
authors.forename: I
authors.initials: I
authors.lastname: Mühldorfer
authors.forename: J
authors.initials: J
authors.lastname: Hacker
mesh_terms: D000818:Animals; D004926:Escherichia coli / Q000235:genetics / Q000254:growth & development / Q000472:pathogenicity*; D017353:Gene Deletion; D005798:Genes, Bacterial / Q000235:genetics; D005816:Genetic Complementation Test; D051379:Mice; D009093:Mucus / Q000382:microbiology*; D012356:RNA, Transfer, Leu / Q000235:genetics* / Q000502:physiology; D001743:Urinary Bladder / Q000382:microbiology*; D014774:Virulence / Q000235:genetics
chemical_list: D012356:RNA, Transfer, Leu