Characterization of two new cuticular genes specifically expressed during the post-ecdysial molting period in Tenebrio molitor.
In a previous study, we have isolated a cDNA, TM-ACP17, coding for a post-ecdysial adult protein of Tenebrio molitor. After screening of a genomic library with TM-ACP17, we report isolation and sequencing of TM-ACP17 gene and a new gene, TM-LPCP29, coding for a larval-pupal protein. These two genes exhibit a common sequence of 15 nucleotides and a characteristic of most cuticular protein genes so far described: an intron interrupting the signal peptide. The deduced aa sequence of TM-LPCP29 exhibits a high percentage of Ala (26.5%) and Val (17.5%) and is highly hydrophobic. In the N-terminal part, the motif VAAPV is repeated ten times. Numerous histidine residues are present in the C- and N-terminal regions. A comparison is made with other cuticle protein sequences. Northern hybridization analysis showed that TM-LPCP29 is present during larval and mainly pupal post-ecdysial cuticle secretion. In-situ hybridization revealed that TM-LPCP29 mRNA is expressed in epidermis and not in muscles or fat body.
Mathelin
J
J
UMR CNRS 5548, Université de Bourgogne, 6 Boulevard Gabriel, 21000, Dijon, France.
Quennedey
B
B
Bouhin
H
H
Delachambre
J
J
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D004247:DNA / Q000737:chemistry / Q000235:genetics; D015870:Gene Expression / Q000235:genetics; D018507:Gene Expression Regulation, Developmental; D005796:Genes / Q000235:genetics; D017344:Genes, Insect / Q000235:genetics*; D016678:Genome; D019476:Insect Proteins / Q000235:genetics*; D007814:Larva / Q000737:chemistry / Q000235:genetics / Q000648:ultrastructure; D008969:Molecular Sequence Data; D018978:Molting / Q000235:genetics*; D011679:Pupa / Q000737:chemistry / Q000235:genetics / Q000648:ultrastructure; D012333:RNA, Messenger / Q000235:genetics; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D013712:Tenebrio / Q000737:chemistry / Q000235:genetics* / Q000648:ultrastructure
C112954:ACP17 protein, Tenebrio molitor; D019476:Insect Proteins; C112955:LPCP29 protein, Tenebrio molitor; D012333:RNA, Messenger; C047663:cuticle proteins, insects; D004247:DNA