[Current status of the application of patch-clamp technique and its combinations with other techniques].
The Patch-clamp technique, as an advanced electrophysiological technique, has been widely used for research in the life science research field. A recent trend is to combine this technique with other techniques, such as the Fura-2 microfluorimetry for measuring the concentration of Ca2+, electrochemical detection with carbon-fiber electrode (i.e. amperometric detection or amperometry), and single-cell reverse transcription of RNA followed by the polymerase chain reaction (RT-PCR). This article is to summarize the current status of the use of patch-clamp technique and its combinations with other techniques in solving problems related with transmembrane signal transduction in neurobiology.
Liu
N J
NJ
Neuroscience Research Center, Beijing Medical University.
Yu
Y X
YX
Han
J S
JS
D000818:Animals; D004563:Electrochemistry; D005434:Flow Cytometry / Q000379:methods; D018408:Patch-Clamp Techniques; D016133:Polymerase Chain Reaction / Q000379:methods; D015398:Signal Transduction