title: Induction of single-strand breaks in lymphocyte DNA of rats exposed to hyperoxia.
abstract: A modified technique of alkaline filter elution was used to evaluate single-strand breaks (SSB) as a measure of DNA lesions in lymphocytes of male Wistar rats exposed to 60 or 80 vol% oxygen, over 23 h/day and for 6 or 5 days, respectively. An acceleration of elution was observed with samples from experiments at both increased levels of oxygen. With proteinase K treatment, a twofold increase in elution rates after exposure to 60 vol% oxygen and a threefold increase at 80 vol% oxygen were indicative of increased occurrence of SSB. This is explained by an involvement of reactive oxygen species in DNA damage under elevated oxygen levels.
authors.forename: R R
authors.initials: RR
authors.lastname: Vangala
authors.forename: K
authors.initials: K
authors.lastname: Kritzler
authors.forename: G
authors.initials: G
authors.lastname: Schöch
authors.forename: H
authors.initials: H
authors.lastname: Topp
mesh_terms: D000818:Animals; D018736:DNA Adducts / Q000378:metabolism; D004249:DNA Damage; D018496:Hyperoxia / Q000378:metabolism*; D008214:Lymphocytes / Q000187:drug effects* / Q000378:metabolism; D008297:Male; D009152:Mutagenicity Tests; D010100:Oxygen / Q000633:toxicity*; D051381:Rats; D017208:Rats, Wistar
chemical_list: D018736:DNA Adducts; D010100:Oxygen