[Expression of apoptosis regulating proteins P 53, c-myc and bcl-2 on CD34+ hematopoietic cells isolated from human bone marrow].
OBJECTIVE
To show the coexpression levels of P 53, c-myc or bcl-2, three key apoptosis-regulating proteins on CD34+HC and bone marrow mononuclear cells (BMMNC).


METHODS
IsolexTM50 or CIMS-100 systems of immunomagnetic bead separation and two-dimensional flow cytometric analysis of the immunofluorescence double staining were used.


RESULTS
With 90%-95% purity of CD34+HC measured by FACS, we found that expression levels of P53, c-myc, and bcl-2 on both CD34+HC and BMMNC were as follows: CD34+/P53 + 0.1-00.8% versus BMMNC 0.1-0.6%; CD34+/c-myc+2.4%-11. 3% versus BMMNC 0.1-0.4%; CD34+/bcl-2+0.5-1.6% versus BMMNC 0.1-0.8%. Coexpression of c-myc and bcl-2 on CD34+HC was significantly higher than those observed on BMMNC respectively.


CONCLUSIONS
It could be speculated that apoptosis-regulating proteins P53, c-myc and bcl-2 play a key role in the control of the survival, proliferation, differentiation, maturation and death in hematopoietic cells of physiological state, suggesting that these proteins balance the production numbers and quality of hematopoietic cells.
Xi
Y
Y
Department of Immunology, 307 Hospital, Academy of Military Medical Sciences, Beijing.
Zhang
S
S
Hao
X
X
D018952:Antigens, CD34; D017209:Apoptosis; D015972:Gene Expression Regulation, Neoplastic; D006412:Hematopoietic Stem Cells / Q000378:metabolism*; D006801:Humans; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000096:biosynthesis*; D016271:Proto-Oncogene Proteins c-myc / Q000096:biosynthesis; D016159:Tumor Suppressor Protein p53 / Q000096:biosynthesis*
D018952:Antigens, CD34; D019253:Proto-Oncogene Proteins c-bcl-2; D016271:Proto-Oncogene Proteins c-myc; D016159:Tumor Suppressor Protein p53