title: Down-regulated expression of transforming growth factor beta 1 mRNA in endometrial carcinoma.
abstract: Transforming growth factor beta1 (TGF-beta1) is a potent modulator of cell proliferation in vitro, and recent studies have demonstrated its overexpression in several different tumours; nevertheless, the molecular mechanisms of TGF-beta1 action on cell growth and differentiation have not been fully elucidated. To clarify the role of TGF-beta and its receptor in human endometrial proliferation and differentiation, TGF-beta1 expression at both the mRNA and protein levels has been evaluated by using Northern blotting and immunohistochemistry, in both normal (atrophic, proliferative and secretory) and neoplastic (adenocarcinoma) endometrial samples. This study demonstrates that TGF-beta1 mRNA expression is dramatically reduced in endometrial carcinomas with respect to non-neoplastic tissues, whereas the immunohistochemical expression of TGF-beta1 is enhanced in the epithelial component of endometrial carcinomas compared with non-neoplastic tissues. These data suggest that TGF-beta1 acts as a paracrine regulator of endometrial cell proliferation and that it may contribute to the carcinogenic mechanisms of endometrial carcinoma.
authors.affiliation: Centro di Studio sui Mitocondri e Metabolismo Energetico, CNR, Bari, Italy.
authors.forename: E
authors.initials: E
authors.lastname: Perlino
authors.forename: G
authors.initials: G
authors.lastname: Loverro
authors.forename: E
authors.initials: E
authors.lastname: Maiorano
authors.forename: T
authors.initials: T
authors.lastname: Giannini
authors.forename: A
authors.initials: A
authors.lastname: Cazzolla
authors.forename: A
authors.initials: A
authors.lastname: Napoli
authors.forename: M G
authors.initials: MG
authors.lastname: Fiore
authors.forename: R
authors.initials: R
authors.lastname: Ricco
authors.forename: E
authors.initials: E
authors.lastname: Marra
authors.forename: L
authors.initials: L
authors.lastname: Selvaggi
mesh_terms: D030201:Activin Receptors, Type I; D000230:Adenocarcinoma / Q000378:metabolism* / Q000473:pathology; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D015152:Blotting, Northern; D015536:Down-Regulation; D016889:Endometrial Neoplasms / Q000378:metabolism* / Q000473:pathology; D004717:Endometrium / Q000378:metabolism; D005260:Female; D018922:HL-60 Cells / Q000378:metabolism; D006801:Humans; D007150:Immunohistochemistry; D007889:Leiomyoma / Q000378:metabolism / Q000473:pathology; D008875:Middle Aged; D009363:Neoplasm Proteins / Q000235:genetics / Q000378:metabolism*; D017346:Protein Serine-Threonine Kinases / Q000378:metabolism; D012333:RNA, Messenger / Q000378:metabolism*; D000077293:Receptor, Transforming Growth Factor-beta Type I; D018125:Receptors, Transforming Growth Factor beta / Q000378:metabolism; D016212:Transforming Growth Factor beta / Q000235:genetics / Q000378:metabolism*; D014407:Tumor Cells, Cultured
chemical_list: D009363:Neoplasm Proteins; D012333:RNA, Messenger; D018125:Receptors, Transforming Growth Factor beta; D016212:Transforming Growth Factor beta; D017346:Protein Serine-Threonine Kinases; D030201:Activin Receptors, Type I; D000077293:Receptor, Transforming Growth Factor-beta Type I