title: Biochemical and structural characterization of triacylglycerol lipase from Penicillium cyclopium.
abstract: An extracellular lipase, active on water-insoluble triacylglycerols, has been isolated from Penicillium cyclopium. The purified enzyme has a molecular mass of 29 kDa by gel filtration and SDS-polyacrylamide gel electrophoresis. It hydrolyzes emulsions of tributyrin, trioctanoin, and olive oil at the same rate as pancreatic lipase and shows very low activity against partial acylglycerols (monooctanoin and dioctanoin) and methyl esters. It is stable at 35 degrees C for 60 min and has maximal activity in a pH range of 8-10. Hydrolysis of triacylglycerols by P. cyclopium lipase is inhibited by detergents such as Triton X-100. Comparison of the sequence of the 20 first amino acid residues of P. cyclopium triacylglycerol lipase with other Penicillium lipases indicates a high homology with previously characterized lipases produced by P. expansum and P. solitum which are enzymes of comparable size and substrate specificity. Conversely, homology between P. cyclopium lipase and P. simplicissimum lipase, a nonspecific lipolytic enzyme, is low. Penicillium cyclopium triacylglycerol lipase shows no homology with P. camembertii lipase which is specific to monoacylglycerol and diacylglycerol.
authors.affiliation: Laboratoire de Chimie Biologique Appliquée, Faculté des Sciences et Techniques de St-Jérôme, Marseille, France.
authors.forename: A
authors.initials: A
authors.lastname: Ibrik
authors.forename: H
authors.initials: H
authors.lastname: Chahinian
authors.forename: N
authors.initials: N
authors.lastname: Rugani
authors.forename: L
authors.initials: L
authors.lastname: Sarda
authors.forename: L C
authors.initials: LC
authors.lastname: Comeau
mesh_terms: D000595:Amino Acid Sequence; D008049:Lipase / Q000032:analysis* / Q000737:chemistry* / Q000378:metabolism; D008969:Molecular Sequence Data; D010407:Penicillium / Q000737:chemistry* / Q000201:enzymology*; D017386:Sequence Homology, Amino Acid; D013379:Substrate Specificity
chemical_list: D008049:Lipase