Multiprimer--PCR for screening of IgH and T-cell receptor rearranged genes in acute lymphoblastic leukemia.
OBJECTIVE
To develop a multiprimer-polymerase chain reaction (multiprimer-PCR) method for detecting immunoglobulin heavy chain (IgH) and T-cell receptor (TCR) rearranged genes in the same amplification.


METHODS
Multiprimer-PCR protocol, a mixture of four different primers used in the same reaction detected IgH CDR-III and TCR V gamma I-J gamma Ligenetic rearrangements in 40 acute lymphoblastic leukemia (ALL) patients.


RESULTS
Thirteen cases were found to have IgH CDR-III and TCR V gamma I-J gamma genetic rearrangements. Ten cases were found to have TCR V gamma I-J gamma genetic rearrangements. Thirteen cases were found to have IgH CDR-III genetic rearrangements. The amplification result of multiprimer-PCR was the same as separate amplification. The sensitivity of multiprimer-PCR was 10(-4)-10(-5) DNA level, which was the same as separate PCR amplification. All positive cases with IgH rearranged gene were confirmed by Southern blot.


CONCLUSIONS
Multiprimer-PCR could screen two distinct rearranged genes in a single amplification, and is more suitable for screening of specimens collected at diagnosis.
Xu
B
B
Department of Hematology, Nanfang Hospital, First Military Medical University, Chian.
Zhou
S
S
Sun
J
J
D000293:Adolescent; D000328:Adult; D002648:Child; D002675:Child, Preschool; D015326:Gene Rearrangement, B-Lymphocyte, Heavy Chain; D015329:Gene Rearrangement, T-Lymphocyte; D006801:Humans; D008875:Middle Aged; D016133:Polymerase Chain Reaction; D054198:Precursor Cell Lymphoblastic Leukemia-Lymphoma / Q000235:genetics*; D011948:Receptors, Antigen, T-Cell / Q000235:genetics*
D011948:Receptors, Antigen, T-Cell