[The relationship between TIL from human primary hepatic carcinoma and prognosis].
OBJECTIVE
To determine whether tumor-infiltrating lymphocytes (TIL) could affect the prognosis for patients with primary hepatic carcinoma (PHC).


METHODS
Tissue distribution, proliferative property and cytotoxicity of TIL were measured in colour medical image analysis, immunohistochemical technique, 3H-thymidine proliferative response and LDH-release assay.


RESULTS
On the basis of infiltrating level of TIL, all patients with PHC were classified into three types in which there can be non-infiltrating, lower infiltrating and high infiltrating. The survival time of patients without TIL was shorter than that of patients with TIL. In addition, their postoperative intrahepatic recurrences were higher than those of the latter. Freshly isolated TIL showed more poor proliferation and cytotoxicity than autologous peripheral blood lymphocytes (PBL) in vitro. TIL activated with anti-CD3 McAb and rIL-2 displayed higher cytotoxicity against fresh autologous hepatic carcinoma cells than against K562 targets.


CONCLUSIONS
Clinically, TIL are associated with the prognosis for patients with PHC, however, it is in the tumor nest that the functions of TIL are impaired or suppressed by some factors locally produced by tumor cells in vivo.
Qin
J
J
44th Hospital PLA, Guiyang.
Han
B
B
Pang
J
J
D000328:Adult; D000368:Aged; D006528:Carcinoma, Hepatocellular / Q000276:immunology / Q000473:pathology*; D002455:Cell Division; D003602:Cytotoxicity, Immunologic; D006801:Humans; D016219:Immunotherapy, Adoptive; D008113:Liver Neoplasms / Q000276:immunology / Q000473:pathology*; D016246:Lymphocytes, Tumor-Infiltrating / Q000276:immunology / Q000473:pathology*; D008875:Middle Aged; D011379:Prognosis