Alternate polyadenylation in human mRNAs: a large-scale analysis by EST clustering.
Alternate polyadenylation is an important post-transcriptional regulatory process now open to large-scale analysis by use of cDNA databases. We clustered 164,000 expressed sequence tags (ESTs) into approximately 15,000 groups and aligned each group to a putative mRNA 3' end. By use of stringent criteria to discard artifactual mRNA extremities, clear evidence for alternate polyadenylation was obtained in 189 of the 1000 EST clusters studied. A number of previously unreported polyadenylation sites were identified, together with possible instances of tissue-specific differential polyadenylation. This study demonstrates that, besides quantitative aspects of gene expression, the distribution of alternate mRNA forms can be analyzed through EST sampling.
Gautheret
D
D
Structural and Genetic Information, Center National de la Recherche Scientifique-E.P. 91, 13 402 Marseille Cedex 20, France. gauthere@igs.cnrs-mrs.fr
Poirot
O
O
Lopez
F
F
Audic
S
S
Claverie
J M
JM
D019295:Computational Biology / Q000379:methods*; D016208:Databases, Factual; D015870:Gene Expression / Q000235:genetics*; D006801:Humans; D011061:Poly A / Q000378:metabolism*; D014176:Protein Biosynthesis; D012333:RNA, Messenger / Q000737:chemistry / Q000378:metabolism*; D012045:Regulatory Sequences, Nucleic Acid
D012333:RNA, Messenger; D011061:Poly A