title: Electron crystallographic analysis of two-dimensional streptavidin crystals coordinated to metal-chelated lipid monolayers.
abstract: Coordination of individual histidine residues located on a protein surface to metal-chelated lipid monolayers is a potentially general method for crystallizing proteins in two dimensions. It was shown recently by Brewster angle microscopy (BAM) that the model protein streptavidin binds via its surface histidines to Cu-DOIDA lipid monolayers, and aggregates into regularly shaped domains that have the appearance of crystals. We have used electron microscopy to confirm that the domains are indeed crystalline with lattice parameters similar to those of the same protein crystallized beneath biotinylated lipid monolayers. Although BAM demonstrates that the two-dimensional protein crystals grown via metal chelation are distinct from the biotin-bound crystals in both microscopic shape and thermodynamic behavior, the two crystal types show similar density projections and the same plane group symmetry.
authors.affiliation: Department of Bioengineering, University of Washington, Seattle 98195, USA.
authors.forename: W
authors.initials: W
authors.lastname: Frey
authors.forename: J
authors.initials: J
authors.lastname: Brink
authors.forename: W R
authors.initials: WR
authors.lastname: Schief
authors.forename: W
authors.initials: W
authors.lastname: Chiu
authors.forename: V
authors.initials: V
authors.lastname: Vogel
mesh_terms: D000327:Adsorption; D001710:Biotin; D003461:Crystallography / Q000379:methods; D004075:Diglycerides; D006639:Histidine; D008024:Ligands; D008853:Microscopy / Q000379:methods; D008854:Microscopy, Electron; D009942:Organometallic Compounds; D011485:Protein Binding; D011487:Protein Conformation; D019809:Streptavidin / Q000737:chemistry*; D013816:Thermodynamics
chemical_list: D004075:Diglycerides; D008024:Ligands; D009942:Organometallic Compounds; C113012:copper 1,2-oleyl-rac-glycero-3-(8-3,6-dioxy)octyl-1-amino-N,N-diacetic acid; D006639:Histidine; D001710:Biotin; D019809:Streptavidin