Staphylococcus aureus causing osteomyelitis binds to a nonapeptide sequence in bone sialoprotein.
Bone sialoprotein is a glycoprotein of the bone and dentine extracellular matrix. This protein consists of 320 amino acids, of which 25% are glutamic and aspartic acid residues. Sialic acid, containing oligosaccharides and tyrosine sulphate residues, supplies additional polyanionic properties. Staphylococcal cells, isolated from patients suffering from infection of bone tissue, bind the bone-derived sialoprotein, an interaction which is specifically inhibited by the recombinant bone sialoprotein core protein. We have previously shown that the 150 N-terminal amino acid residues of bone sialoprotein are responsible for the binding to staphylococcal cells. By using recombinant deleted variants of bone sialoprotein and synthetic peptides, we have now localized the staphylococcal binding site to less than 10 residues within the N-terminal part of the protein.
Rydén
C
C
Department of Medical and Physiological Chemistry, University of Uppsala, BMC, Box 575, S-751 23 Uppsala, Sweden.
Tung
H S
HS
Nikolaev
V
V
Engström
A
A
Oldberg
A
A
D000595:Amino Acid Sequence; D001665:Binding Sites; D005982:Glutathione Transferase / Q000235:genetics; D006801:Humans; D058574:Integrin-Binding Sialoprotein; D008969:Molecular Sequence Data; D010019:Osteomyelitis / Q000382:microbiology*; D010455:Peptides / Q000494:pharmacology; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis / Q000235:genetics / Q000378:metabolism; D017384:Sequence Deletion; D012795:Sialoglycoproteins / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism*; D013203:Staphylococcal Infections / Q000382:microbiology*; D013211:Staphylococcus aureus / Q000166:cytology / Q000302:isolation & purification / Q000378:metabolism*
C546871:IBSP protein, human; D058574:Integrin-Binding Sialoprotein; D010455:Peptides; D011993:Recombinant Fusion Proteins; D012795:Sialoglycoproteins; D005982:Glutathione Transferase