title: Immunoradiometric assay for the N-terminal fragment of proatrial natriuretic peptide in human plasma.
abstract: Recently, the N-terminal fragment of proatrial natriuretic peptide (N-terminal proANP) has been proposed as a marker of chronic congestive heart failure. In this study, we established a two-step immunoradiometric assay using monoclonal antibodies and synthetic N-terminal proANP (1-67) as a standard. It allows us to measure plasma N-terminal proANP in only 4 h without prior extraction. The detection limit of this assay was 15 pmol/L for a 100 microL sample of plasma. Within-run CVs ranged from 1.7% to 2.9% and between-run CVs ranged from 4.2% to 5.1%. The dilution curves of plasma samples showed good linearity and analytical recovery was 89-104%. The mean (+/-SD) N-terminal proANP in plasma of 33 healthy subjects was 188 (+/-71) pmol/L and 1030 (+/-411) pmol/L in 25 patients with heart failure. Our immunoradiometric assay is rapid and precise enough for routine determination of N-terminal proANP in human plasma.
authors.affiliation: Diagnostic Science Division, Shionogi & Company, Osaka, Japan. yoshito.numata@shionogi.co.jp
authors.forename: Y
authors.initials: Y
authors.lastname: Numata
authors.forename: K
authors.initials: K
authors.lastname: Dohi
authors.forename: A
authors.initials: A
authors.lastname: Furukawa
authors.forename: S
authors.initials: S
authors.lastname: Kikuoka
authors.forename: H
authors.initials: H
authors.lastname: Asada
authors.forename: T
authors.initials: T
authors.lastname: Fukunaga
authors.forename: Y
authors.initials: Y
authors.lastname: Taniguchi
authors.forename: K
authors.initials: K
authors.lastname: Sasakura
authors.forename: T
authors.initials: T
authors.lastname: Tsuji
authors.forename: K
authors.initials: K
authors.lastname: Inouye
authors.forename: M
authors.initials: M
authors.lastname: Yoshimura
authors.forename: H
authors.initials: H
authors.lastname: Itoh
authors.forename: M
authors.initials: M
authors.lastname: Mukoyama
authors.forename: H
authors.initials: H
authors.lastname: Yasue
authors.forename: K
authors.initials: K
authors.lastname: Nakao
mesh_terms: D000911:Antibodies, Monoclonal / Q000276:immunology; D009320:Atrial Natriuretic Factor / Q000097:blood* / Q000276:immunology; D015415:Biomarkers / Q000097:blood; D002850:Chromatography, Gel; D003429:Cross Reactions; D004355:Drug Stability; D004492:Edetic Acid; D000939:Epitopes / Q000276:immunology; D006333:Heart Failure / Q000097:blood; D006461:Hemolysis; D006801:Humans; D015592:Immunoradiometric Assay / Q000379:methods; D011498:Protein Precursors / Q000097:blood* / Q000276:immunology; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
chemical_list: D000911:Antibodies, Monoclonal; D015415:Biomarkers; D000939:Epitopes; C099254:N-terminal proatrial natriuretic peptide; D011498:Protein Precursors; D009320:Atrial Natriuretic Factor; D004492:Edetic Acid