[Flow cytometric DNA analysis and MR imaging of salivary gland tumors].
We correlated the histopathology of 32 cases of salivary gland tumors with MR imaging and flow cytometric DNA analysis. All malignant tumors were invasive and/or had an ill-defined margin. Fifty-seven percent of the pleomorphic adenomas were shown as a high signal intensity area on T2 weighted images. Fifty-six percent of Warthin tumors were shown as a low or iso signal intensity area on T2 weighted images associated with the cystic portion. Sixty-seven percent of malignant tumors were characterized by DNA aneuploidy and/or a total percentage of the S-phase fraction plus the G2 + M fraction higher than 10%. It was less than 10% and the Ki-67 positive fraction was higher than 20% in all pleomorphic adenomas. The Ki-67 positive fraction was less than 20% in 78% of Warthin tumors. The histopathology of all malignant tumors, 86% of the pleomorphic adenomas, and 89% of Warthin tumors was correctly predicted by the combination of MR images and flow cytometry. We suggest that the MR images combined with flow cytometric analysis of fine needle aspiration-derived materials is useful in the pre-operative histopathologic diagnosis of salivary gland tumors.
Horii
A
A
Department of Otolaryngology, Osaka University Medical School.
Yoshida
J
J
Honjo
Y
Y
Mitani
K
K
Hattori
K
K
Kubo
T
T
Sakai
M
M
Okamoto
S
S
Takashima
S
S
D000235:Adenolymphoma / Q000175:diagnosis* / Q000235:genetics / Q000473:pathology; D008949:Adenoma, Pleomorphic / Q000175:diagnosis* / Q000235:genetics / Q000473:pathology; D000782:Aneuploidy; D002453:Cell Cycle; D004273:DNA, Neoplasm / Q000032:analysis*; D005434:Flow Cytometry; D006801:Humans; D019394:Ki-67 Antigen / Q000032:analysis; D008279:Magnetic Resonance Imaging; D012189:Retrospective Studies; D012468:Salivary Gland Neoplasms / Q000175:diagnosis* / Q000235:genetics / Q000473:pathology
D004273:DNA, Neoplasm; D019394:Ki-67 Antigen