title: Evaluation of the gamma-interferon assay for eradication of tuberculosis in a goat herd.
abstract: OBJECTIVE
  To evaluate the usefulness of the gamma-interferon assay in the diagnosis of caprine tuberculosis in comparison with a single intradermal tuberculin test, and to obtain a group of animals free from this infection in a herd with a high prevalence.
  
  
  METHODS
  An immunological study involving four serial comparative gamma-interferon and single intradermal tuberculin tests.
  
  
  METHODS
  A herd of 87 goats of Guadarrama breed.
  
  
  METHODS
  Serial testing and segregation of animals.
  
  
  RESULTS
  We found that the number of infections detected by the gamma-interferon test was considerably greater than the number detected by the single intradermal tuberculin test. A group of 10 animals was negative to both tests in two consecutive rounds and three kids were negative in the last round of testing.
  
  
  CONCLUSIONS
  Gamma-interferon assay is appropriate for diagnosis and eradication of tuberculosis in goats. This test is able to detect early Mycobacterium bovis infection. Avian reactors with simultaneous increased reaction to bovine PPD in the gamma-interferon assay (designated as avian reactors) should be considered test positive for M bovis. By serial testing with the gamma-interferon and the single intradermal tuberculin tests, and a policy of segregation of kids at birth, it is possible to achieve a group of animals test negative for tuberculosis from a herd of goats with high immunoreactivity to this infection.
authors.affiliation: Departmento do Patología Animal I (Sanidad Animal), Facultad de Veterinaria, Universidad Complutense de Madrid, Spain.
authors.forename: E
authors.initials: E
authors.lastname: Liébana
authors.forename: A
authors.initials: A
authors.lastname: Aranaz
authors.forename: J J
authors.initials: JJ
authors.lastname: Urquía
authors.forename: A
authors.initials: A
authors.lastname: Mateos
authors.forename: L
authors.initials: L
authors.lastname: Domínguez
mesh_terms: D000818:Animals; D005069:Evaluation Studies as Topic; D005260:Female; D015511:Goat Diseases / Q000175:diagnosis* / Q000453:epidemiology / Q000276:immunology; D006041:Goats; D007111:Immunity, Cellular; D007371:Interferon-gamma / Q000097:blood*; D008297:Male; D009163:Mycobacterium bovis; D015995:Prevalence; D012680:Sensitivity and Specificity; D014374:Tuberculin Test / Q000662:veterinary; D014376:Tuberculosis / Q000175:diagnosis / Q000453:epidemiology / Q000276:immunology / Q000662:veterinary*
chemical_list: D007371:Interferon-gamma