Isolation, structural analysis and mapping of the functional gene of human ribosomal protein S26.
The nucleotide sequence of the gene of human ribosomal protein S26 has been assembled from cDNA and genomic PCR-amplified DNA fragments, and its transcription start site has been determined by primer extension. The gene is composed of four exons and three introns spanning 2027bp. Like other ribosomal protein genes of vertebrates, this gene contains a short first exon corresponding exactly to the short untranslated 5'- UTR. Its transcription start site is embedded in a polypyrimidine tract. Using PCR on DNAs from hybrid cell lines with a different set of human chromosomes, the intron-containing gene of ribosomal protein S26 was mapped to human chromosome 12.
Filipenko
M L
ML
Department of Molecular Biology of Gene, Novosibirsk Institute of Bioorganic Chemistry, Siberian Branch of the Russian Academy of Sciences, 630090, Novosibirsk, Russia. max@ns.niboch.nsc.ru
Vinichenko
N A
NA
Karpova
G G
GG
Mertvetsov
N P
NP
Amaldi
F
F
D001483:Base Sequence; D002874:Chromosome Mapping; D002881:Chromosomes, Human, Pair 12 / Q000235:genetics; D003001:Cloning, Molecular; D004247:DNA / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification; D017931:DNA Primers; D005784:Gene Amplification; D005796:Genes / Q000235:genetics* / Q000502:physiology; D016678:Genome; D006367:HeLa Cells; D006801:Humans; D006822:Hybrid Cells; D007438:Introns / Q000235:genetics; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D011401:Promoter Regions, Genetic / Q000235:genetics / Q000502:physiology; D012269:Ribosomal Proteins / Q000737:chemistry / Q000235:genetics* / Q000302:isolation & purification*; D017422:Sequence Analysis, DNA; D016385:TATA Box / Q000235:genetics; D014158:Transcription, Genetic / Q000235:genetics
D017931:DNA Primers; C489203:RPS26 protein, human; D012269:Ribosomal Proteins; D004247:DNA