Effects of chitosan-coated dialdehyde cellulose, a newly developed oral adsorbent, on glomerulonephritis induced by anti-Thy-1 antibody in rats.
The effects of chitosan-coated dialdehyde cellulose (chitosan DAC), a newly developed oral adsorbent for urea and ammonia, were examined in a glomerulonephritis model in rats. Mesangial proliferative glomerulonephritis accompanied with proteinuria was induced by an intravenous injection of anti-rat Thy-1.1 monoclonal antibody (OX-7). The proliferation of mesangial cells and an accumulation of extracellular matrix components such as type I collagen and fibronectin were observed in the glomeruli 9 days after OX-7 injection; these were improved in rats fed a diet containing chitosan DAC (10% content) for 9 days compared with those in rats fed a normal diet. Chitosan DAC treatment decreased the elevated urinary protein and blood urea nitrogen at days 8-9 to the normal levels; the increased fecal excretion of nitrogen might participate in this phenomenon. In addition, chitosan DAC treatment showed an increase in fecal water content associated with a decrease in urinary volume. These therapeutic effects may be due to the reduction of proteinic factor expression and the compensational function of chitosan DAC for kidney. These results suggest that chitosan DAC treatment may be useful for ameliorating mesangial proliferative glomerulonephritis.
Yagi
M
M
Pharmaceutical Research Laboratory, Kirin Brewery Co. Ltd, Takasaki, Gunma, Japan. m-yagi@kirin.co.jp
Kato
S
S
Nishitoba
T
T
Sato
H
H
Kobayashi
N
N
Iinuma
N
N
Nagano
N
N
D000327:Adsorption; D000818:Animals; D000911:Antibodies, Monoclonal / Q000633:toxicity*; D001806:Blood Urea Nitrogen; D002482:Cellulose / Q000008:administration & dosage / Q000031:analogs & derivatives*; D002686:Chitin / Q000008:administration & dosage / Q000031:analogs & derivatives*; D048271:Chitosan; D004195:Disease Models, Animal; D015432:Glomerulonephritis, Membranoproliferative / Q000139:chemically induced / Q000188:drug therapy* / Q000652:urine; D007668:Kidney / Q000473:pathology; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D011507:Proteinuria / Q000188:drug therapy / Q000209:etiology / Q000652:urine; D051381:Rats; D011921:Rats, Inbred WKY; D018800:Thy-1 Antigens / Q000276:immunology*
D000911:Antibodies, Monoclonal; D018800:Thy-1 Antigens; D002686:Chitin; D002482:Cellulose; D048271:Chitosan