Alterations in total iron, zinc, and calcium levels and their influence on the hepatic activities of gamma-glutamyl transferase and glucose-6-phosphatase in the host bearing transplantable murine lymphoma.
The levels of iron, zinc, and calcium in liver as well as serum, together with the enzymatic activities of gamma-glutamyl transferase (GGT, EC 2.3.2.2) and glucose-6-phosphatase (G-6-Pase, EC 3.1.3.9) in liver, were critically monitored over various periods in male Swiss albino mice bearing Dalton's lymphoma (DL), a transplantable ascites-producing tumor. Both hepatic and serum contents of iron, zinc, and calcium were found to be maximally elevated (p < 0.001) on day 15 after tumor transplantation as compared with their contents in normal animals. There was a gradual increase in the activity of GGT in liver in lymphoma-bearing mice in comparison with their normal counterparts, which showed a maximum peak (p < 0.001) on day 15, followed by a continuous and sharp fall. Hepatic G-6-Pase activity was found to decrease continuously throughout the progression of lymphoma as compared with its levels to normal animals. Tumor-cell counts in peritoneal lymph fluids of mice containing DL yielded a maximum count of 155.7 x 10(3) cells/mm3 on day 15. A significant correlation was observed among the levels of different metals, enzymatic activities, and tumor-cell counts at different periods of study. From these results, it can be concluded that the metals studied may have a role in initiating and controlling cellular proliferations, through their effects on modulating the activities of the possibly preneoplastic and neoplastic marker enzymes named above.
Bishayee
A
A
Department of Pharmaceutical Technology, Jadavpur University, Calcutta, India
Kulkarni
S P
SP
Roy
K
K
Chatterjee
M
M
D000818:Animals; D014408:Biomarkers, Tumor / Q000097:blood / Q000378:metabolism*; D002118:Calcium / Q000097:blood / Q000378:metabolism*; D002455:Cell Division / Q000502:physiology; D005952:Glucose-6-Phosphatase / Q000378:metabolism*; D007501:Iron / Q000097:blood / Q000378:metabolism*; D008099:Liver / Q000201:enzymology* / Q000378:metabolism*; D008223:Lymphoma / Q000097:blood / Q000201:enzymology / Q000378:metabolism*; D008297:Male; D051379:Mice; D008811:Mice, Inbred DBA; D009368:Neoplasm Transplantation; D015032:Zinc / Q000097:blood / Q000378:metabolism*; D005723:gamma-Glutamyltransferase / Q000378:metabolism*
D014408:Biomarkers, Tumor; D007501:Iron; D005723:gamma-Glutamyltransferase; D005952:Glucose-6-Phosphatase; D015032:Zinc; D002118:Calcium