PCR-SSCP of rDNA for the identification of Trichinella isolates from mainland china.
A polymerase chain reaction (PCR)-based single strand conformation polymorphism (SSCP) analysis of the expansion segment 5 (domain IV) of the large subunit of ribosomal DNA was employed to characterize seven isolates of Trichinella from China (A-G), including six of pig origin from regions in Dengxian (A), Tianjin (B), Harbin (D), Baoshan (E), Xinye (F) and Xian (G), and one of canine origin from Changchun (C). Isolates A, D, E, F and G were classified as Trichinella spiralis based on SSCP patterns, while the patterns for isolates B and C were consistent with those of Trichinella nativa or Trichinella T6. The results were supported by random amplified polymorphic DNA (RAPD) analysis using five decamer primers and were in accordance with ecological information for the isolates. Single strand conformation polymorphism results also allowed the direct display of mutational sequence variation in the expansion segment among the five isolates of T. spiralis from China, indicating its usefulness for studying population variation within that species.
Gasser
R B
RB
Department of Veterinary Science, The University of Melbourne, Victoria, Australia.
Zhu
X Q
XQ
Monti
J R
JR
Dou
L
L
Cai
X
X
Pozio
E
E
D000818:Animals; D000835:Animals, Wild; D002681:China; D018509:DNA, Helminth / Q000032:analysis* / Q000235:genetics; D004275:DNA, Ribosomal / Q000032:analysis* / Q000235:genetics; D004283:Dog Diseases; D004285:Dogs; D018482:Muscle, Skeletal / Q000469:parasitology*; D016133:Polymerase Chain Reaction / Q000379:methods*; D018807:Polymorphism, Single-Stranded Conformational; D013552:Swine; D013553:Swine Diseases; D014234:Trichinella / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D014235:Trichinellosis / Q000175:diagnosis / Q000469:parasitology / Q000662:veterinary*
D018509:DNA, Helminth; D004275:DNA, Ribosomal