title: Functional similarity between archaeal and bacterial CorA magnesium transporters.
abstract: The constitutively expressed CorA Mg2+ transporter is the major Mg2+ influx system of Salmonella typhimurium and Escherichia coli. Genomic sequence data indicated the presence of a homolog in the archaeal organism Methanococcus jannaschii. The putative M. jannaschii CorA was expressed in an Mg2+-transport-deficient strain of S. typhimurium to determine its functional characteristics. The archaeal CorA homolog is a functional Mg2+ uptake system when expressed in S. typhimurium and has properties which are highly similar to those of the normal CorA transporter of S. typhimurium despite having a low level of sequence identity with the protein and being expressed in a lipid membrane of quite different composition than normal. This implies that the overall function of the proteins is the same and further suggests that their structures are very similar.
authors.affiliation: Department of Biology, University of Texas at Arlington, Arlington, Texas 76019, USA.
authors.forename: R L
authors.initials: RL
authors.lastname: Smith
authors.forename: E
authors.initials: E
authors.lastname: Gottlieb
authors.forename: L M
authors.initials: LM
authors.lastname: Kucharski
authors.forename: M E
authors.initials: ME
authors.lastname: Maguire
mesh_terms: D000595:Amino Acid Sequence; D019843:Archaeal Proteins / Q000502:physiology*; D001426:Bacterial Proteins; D002118:Calcium / Q000494:pharmacology; D002352:Carrier Proteins / Q000187:drug effects / Q000502:physiology*; D027682:Cation Transport Proteins; D004305:Dose-Response Relationship, Drug; D004926:Escherichia coli / Q000502:physiology*; D017136:Ion Transport; D008274:Magnesium / Q000378:metabolism* / Q000494:pharmacology; D017017:Methanococcus / Q000502:physiology*; D008969:Molecular Sequence Data; D012486:Salmonella typhimurium / Q000502:physiology*; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid
chemical_list: D019843:Archaeal Proteins; D001426:Bacterial Proteins; D002352:Carrier Proteins; D027682:Cation Transport Proteins; C072716:CorA protein, Salmonella; D008274:Magnesium; D002118:Calcium