p300/CREB binding protein-related protein p270 is a component of mammalian SWI/SNF complexes.
p300 and the closely related CREB binding protein (CBP) are transcriptional adaptors that are present in intracellular complexes with TATA binding protein (TBP) and bind to upstream activators including p53 and nuclear hormone receptors. They have intrinsic and associated histone acetyltransferase activity, suggesting that chromatin modification is an essential part of their role in regulating transcription. Detailed characterization of a panel of antibodies raised against p300/CBP has revealed the existence of a 270-kDa cellular protein, p270, distinct from p300 and CBP but sharing at least two independent epitopes with p300. The subset of p300/CBP-derived antibodies that cross-reacts with p270 consistently coprecipitates a series a cellular proteins with relative molecular masses ranging from 44 to 190 kDa. Purification and analysis of various proteins in this group reveals that they are components of the human SWI/SNF complex and that p270 is an integral member of this complex.
Dallas
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PB
Fels Institute for Cancer Research and Molecular Biology, Temple University School of Medicine, Philadelphia, Pennsylvania 19140, USA.
Cheney
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IW
Liao
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DW
Bowrin
V
V
Byam
W
W
Pacchione
S
S
Kobayashi
R
R
Yaciuk
P
P
Moran
E
E
D000251:Adenosine Triphosphatases / Q000378:metabolism; D000595:Amino Acid Sequence; D000936:Antigen-Antibody Complex / Q000378:metabolism; D050882:CREB-Binding Protein; D004265:DNA Helicases; D004268:DNA-Binding Proteins / Q000378:metabolism; D018604:Epitope Mapping; D006367:HeLa Cells; D006801:Humans; D046911:Macromolecular Substances; D008969:Molecular Sequence Data; D008970:Molecular Weight; D009687:Nuclear Proteins / Q000378:metabolism*; D035181:TATA-Box Binding Protein; D015534:Trans-Activators; D014157:Transcription Factors / Q000032:analysis* / Q000737:chemistry / Q000378:metabolism*
C112476:ARID1A protein, human; D000936:Antigen-Antibody Complex; D004268:DNA-Binding Proteins; D046911:Macromolecular Substances; D009687:Nuclear Proteins; C101681:SMARCC1 protein, human; C101682:SMARCC2 protein, human; D035181:TATA-Box Binding Protein; D015534:Trans-Activators; D014157:Transcription Factors; D050882:CREB-Binding Protein; C494363:CREBBP protein, human; D000251:Adenosine Triphosphatases; C084108:SMARCA4 protein, human; D004265:DNA Helicases