Establishment of complement-resistant retroviral vector by homologous restriction factor 20 gene.
Homologous restriction factor 20 (HRF20, CD59) is one of the complement regulatory factors. In this study, the complement-resistant retroviral vector, which possesses the HRF20 gene as a selection gene, was constructed and examined. The virus-producing cell, transduced with complement-resistant retroviral vector, was established after complement-dependent selection. NIH3T3 and PK15 cells transduced with this virus-producing cell were successfully selected by complement-dependent selection, which showed significant expression of the HRF20 antigen. In addition, these cells, transduced with tissue plasminogen activator (tPA) cDNA using complement-resistant retroviral vector, expressed tPA antigen after complement-dependent selection. These findings suggest that complement-resistant retroviral vector can be used for the double transduction of HRF20, as well as other genes.
Hayashi
S
S
Department of Surgery II, Nagoya University, School of Medicine, Japan.
Emi
N
N
Okada
H
H
Nagasaka
T
T
Yokoyama
I
I
Takagi
H
H
D016475:3T3 Cells; D000818:Animals; D001798:Blood Proteins / Q000235:genetics*; D018957:CD59 Antigens; D002352:Carrier Proteins / Q000235:genetics*; D002460:Cell Line; D003169:Complement Inactivator Proteins / Q000235:genetics*; D004797:Enzyme-Linked Immunosorbent Assay; D018014:Gene Transfer Techniques; D005822:Genetic Vectors; D006801:Humans; D051379:Mice; D012190:Retroviridae; D010959:Tissue Plasminogen Activator / Q000378:metabolism
D001798:Blood Proteins; D018957:CD59 Antigens; D002352:Carrier Proteins; D003169:Complement Inactivator Proteins; C051590:CD59 protein, human; D010959:Tissue Plasminogen Activator