[Consecutive study on alveolar macrophage release of tumor necrosis factor-alpha and platelet-derived growth factor in bleomycin-induced pulmonary fibrosis in rats].
OBJECTIVE
Observe consecutively alveolar macrophage (AM) release of tumor necrosis factor-alpha (TNF-alpha) and platelet-derived growth factor (PDGF in bleomycin-induced pulmonary fibrosis in rats.


METHODS
ELISA method for TNF-alpha quantitation; Bioassay method for PDGF assessment.


RESULTS
(1) AM TNF-alpha release was elevated on 3rd day, peaked on 7th day and then remained at higher level than that in control. (2) AM PDGF release was elevated on 3rd day, also peaked on 7th day, but then decreased to a near normal level.


CONCLUSIONS
AM might play a role in the pathogenesis of pulmonary fibrosis through release of TNF-alpha and PDGF.
Chen
B
B
Respiratory Disease Institute, China Medical University, Shenyang.
Jiang
I
I
Zhao
H
H
D000818:Animals; D001761:Bleomycin; D016676:Macrophages, Alveolar / Q000378:metabolism*; D010982:Platelet-Derived Growth Factor / Q000378:metabolism*; D011658:Pulmonary Fibrosis / Q000139:chemically induced / Q000378:metabolism*; D051381:Rats; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism*
D010982:Platelet-Derived Growth Factor; D014409:Tumor Necrosis Factor-alpha; D001761:Bleomycin