title: [Current status of the application of patch-clamp technique and its combinations with other techniques].
abstract: The Patch-clamp technique, as an advanced electrophysiological technique, has been widely used for research in the life science research field. A recent trend is to combine this technique with other techniques, such as the Fura-2 microfluorimetry for measuring the concentration of Ca2+, electrochemical detection with carbon-fiber electrode (i.e. amperometric detection or amperometry), and single-cell reverse transcription of RNA followed by the polymerase chain reaction (RT-PCR). This article is to summarize the current status of the use of patch-clamp technique and its combinations with other techniques in solving problems related with transmembrane signal transduction in neurobiology.
authors.affiliation: Neuroscience Research Center, Beijing Medical University.
authors.forename: N J
authors.initials: NJ
authors.lastname: Liu
authors.forename: Y X
authors.initials: YX
authors.lastname: Yu
authors.forename: J S
authors.initials: JS
authors.lastname: Han
mesh_terms: D000818:Animals; D004563:Electrochemistry; D005434:Flow Cytometry / Q000379:methods; D018408:Patch-Clamp Techniques; D016133:Polymerase Chain Reaction / Q000379:methods; D015398:Signal Transduction