title: Recombinant mouse-human chimeric antibodies as calibrators in immunoassays that measure antibodies to Toxoplasma gondii.
abstract: In the present study, we examined the feasibility of using recombinant antibodies containing murine variable regions and human constant regions as calibrators or controls in immunoassays. As a model system, we chose the Abbott IMx Toxo immunoglobulin M (IgM) and Toxo IgG assays designed to detect antibodies to Toxoplasma gondii. Two mouse monoclonal antibodies were selected based on their reactivity to the T. gondii antigens P30 and P66. Heavy- and light-chain variable-region genes were cloned from both hybridomas and transferred into immunoglobulin expression vectors containing human kappa and IgG1 or IgM constant regions. The constructs were stably transfected into Sp2/0-Ag14 cells. In the IMx Toxo IgG assay, immunoreactivity of the anti-P30 chimeric IgG1 antibody paralleled that of the positive human plasma-derived assay calibrators. Signal generated with the anti-P66 chimeric IgG1 antibody was observed to plateau below the maximal reactivity observed for the assay calibrator. Examination of the IgM chimeric antibodies in the IMx Toxo IgM assay revealed that both the anti-P30 and anti-P66 antibodies matched the assay index calibrator manufactured with human Toxo IgM-positive plasma. When evaluated with patient samples, the correlation between results obtained with the chimeric antibody calibrators and the positive human plasma calibrators was > or =0.985. These data demonstrate that chimeric mouse-human antibodies are a viable alternative to high-titer positive human plasma for the manufacture of calibrators and controls for diagnostic assays.
authors.affiliation: Abbott Diagnostics Division, Abbott Laboratories, North Chicago, Illinois 60064, USA. john.hackett@add.ssw.abbott.com
authors.forename: J
authors.initials: J
authors.lastname: Hackett
authors.forename: J
authors.initials: J
authors.lastname: Hoff-Velk
authors.forename: A
authors.initials: A
authors.lastname: Golden
authors.forename: J
authors.initials: J
authors.lastname: Brashear
authors.forename: J
authors.initials: J
authors.lastname: Robinson
authors.forename: M
authors.initials: M
authors.lastname: Rapp
authors.forename: M
authors.initials: M
authors.lastname: Klass
authors.forename: D H
authors.initials: DH
authors.lastname: Ostrow
authors.forename: W
authors.initials: W
authors.lastname: Mandecki
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D000911:Antibodies, Monoclonal; D000913:Antibodies, Protozoan / Q000032:analysis*; D000918:Antibody Specificity; D001483:Base Sequence; D002138:Calibration; D002478:Cells, Cultured; D004306:Dose-Response Relationship, Immunologic; D006801:Humans; D007074:Immunoglobulin G / Q000032:analysis* / Q000235:genetics; D007075:Immunoglobulin M / Q000032:analysis* / Q000235:genetics; D051379:Mice; D008969:Molecular Sequence Data; D011933:Reagent Kits, Diagnostic; D011993:Recombinant Fusion Proteins / Q000032:analysis*; D014122:Toxoplasma / Q000276:immunology* / Q000302:isolation & purification
chemical_list: D000911:Antibodies, Monoclonal; D000913:Antibodies, Protozoan; D007074:Immunoglobulin G; D007075:Immunoglobulin M; D011933:Reagent Kits, Diagnostic; D011993:Recombinant Fusion Proteins