Mediation of the cytotoxicity of lanostanoids and steroids of Ganoderma tsugae through apoptosis and cell cycle.
A new lanostanoid ester glucoside, 3 alpha-acetoxy-5 alpha-lanosta-8,24-dien-21-oic acid ester beta-d-glucoside (1), and a known steroid, 2 beta,3 alpha,9 alpha-trihydroxy-5 alpha-ergosta-7,22-diene (2), were isolated from the fruit bodies of Ganoderma tsugae and their structures determined by spectroscopic methods. To study the cytotoxicity of 1 and 2, the changes of DNA content in human hepatocytes (Hep 3B) were studied. A sub-G1 cell stage was drastically increased after 24-h incubation with 1 (24 micrograms/mL). Compound 2 (100 micrograms/mL) inhibited the cell cycle progression of Hep 3B cells at the G2/M phase with an IC50 value of about 87.1 micrograms/mL. These results indicate that 1 causes cell death by apoptosis and 2 may possess the activity of cell cycle inhibition.
Gan
K H
KH
Department of Biochemistry, Kaohsiung Medical College, Taiwan, Republic of China.
Fann
Y F
YF
Hsu
S H
SH
Kuo
K W
KW
Lin
C N
CN
D000972:Antineoplastic Agents, Phytogenic / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects*; D002453:Cell Cycle / Q000187:drug effects*; D002776:Cholestanes / Q000302:isolation & purification* / Q000494:pharmacology; D004247:DNA / Q000737:chemistry / Q000187:drug effects; D005434:Flow Cytometry; D005960:Glucosides / Q000302:isolation & purification* / Q000494:pharmacology; D006801:Humans; D009682:Magnetic Resonance Spectroscopy; D013058:Mass Spectrometry; D008968:Molecular Conformation; D010946:Plants, Medicinal / Q000737:chemistry*; D013055:Spectrophotometry, Infrared; D013056:Spectrophotometry, Ultraviolet; D013256:Steroids / Q000633:toxicity*; D014407:Tumor Cells, Cultured
C112367:3-acetoxy-5-lanosta-8,24-dien-21-oic acid ester glucoside; D000972:Antineoplastic Agents, Phytogenic; D002776:Cholestanes; D005960:Glucosides; D013256:Steroids; D004247:DNA