[Detection of serum HCV RNA and HBV DNA in patients on hemodialysis].
In order to assess the relationship between seropositivity and potential infectivity caused by hepatitis C virus (HCV) and hepatitis B virus (HBV) in patients undergoing maintenance hemodialysis, 22 patients in our dialysis unit were evaluated by using nested RT-PCR to detect serum HCV RNA, ELISA to detect serum anti-HCV antibodies, dot blot to detect serum HBV DNA and RIA to detect HBV serum markers. Twenty-seven percent of the patients (6 of 22) was anti-HCV-positive. Of the 6 anti-HCV-positive patients, HCV RNA was detected in 3 (33%) patients. Among the anti-HCV-negative patients, HCV RNA was detected in one (6%) of the 16 patients. Fourteen percent (3 of 22) was HBV DNA-positive. Of the 3 HBV DNA-positive patients, HBeAG was detected in all of them. Among the nineteen HBV DNA-negative patients, no HBeAg was detected, but anti-HBc was detected in 12 (63%) of the 19 patients. Five of the seven patients with HCV infection (HCV-Ab and/or HCV RNA-positive) had evidence of additional present or past hepatitis B virus infection (4 of the 5 patients were anti-HBc-positive and one of the 5 was HBV DNA, HBeAg, HBsAg, and anti-HBc-positive). However, no HCV RNA and HBV DNA were found to coexist in the same patient in this study. These data indicate that many patients on hemodialysis have hepatitis C or B viremia and more attention should be paid to this problem.
Wei
L
L
Institute of Nephrology, Beijing Medical University.
Wang
M
M
Zhang
Q
Q
D004279:DNA, Viral / Q000097:blood*; D005260:Female; D016174:Hepacivirus / Q000235:genetics*; D006509:Hepatitis B / Q000209:etiology; D006514:Hepatitis B Surface Antigens / Q000097:blood; D006515:Hepatitis B virus / Q000235:genetics*; D006526:Hepatitis C / Q000209:etiology; D018937:Hepatitis C Antibodies / Q000097:blood; D006801:Humans; D008297:Male; D008875:Middle Aged; D012367:RNA, Viral / Q000097:blood*; D006435:Renal Dialysis / Q000009:adverse effects*; D015163:Superinfection
D004279:DNA, Viral; D006514:Hepatitis B Surface Antigens; D018937:Hepatitis C Antibodies; D012367:RNA, Viral