Effect of carboxy-PTIO, a nitric oxide scavenger, on the proliferation of murine hematopoietic progenitor cells in vitro.
We investigated the effect of 2-(4-carboxyphenyl)-4,4,5,5-tetramethylimidazoline-1-oxyl 3-oxide (c-PTIO), a specific nitric oxide scavenger and a stable radical compound, on the proliferation of murine hematopoietic progenitor cells in vitro. The c-PTIO promoted colony formation by erythropoietin and either interleukin-6 (IL-6) or the c-kit ligand/stem cell factor (SCF) in a methylcellulose culture, where the number of colonies increased 2.2-fold and 1.7-fold, respectively. During the addition of c-PTIO to the liquid cultures of murine bone marrow cells containing a combination of IL-6 and SCF, colony-forming cells in vitro (CFC) and the colony-forming unit in the spleen (CFU-S) increased about 1.8-fold and 1.7-fold, respectively, higher than the control culture after 7 day of culture. When c-PTIO was added twice at days 0 and 2 during the culture, 3.6-fold and 1.7-fold increases over the control were observed in the number of CFC and CFU-S. These results suggest the possibility that c-PTIO regulates the proliferation and differentiation of hematopoietic stem/progenitor cells in vitro.
Kashiwakura
I
I
Laboratory of Radiopharmaceutical Sciences, Hokkaido College of Pharmacy, Otaru, Japan.
Kuwabara
M
M
Murakami
M
M
Hayase
Y
Y
Takagi
Y
Y
D000818:Animals; D001565:Benzoates / Q000494:pharmacology*; D001854:Bone Marrow Cells / Q000166:cytology / Q000187:drug effects; D002455:Cell Division / Q000187:drug effects; D003114:Colony-Forming Units Assay; D006412:Hematopoietic Stem Cells / Q000166:cytology / Q000187:drug effects*; D007093:Imidazoles / Q000494:pharmacology*; D007377:Interleukin-3 / Q000494:pharmacology; D015850:Interleukin-6 / Q000494:pharmacology; D008297:Male; D051379:Mice; D009569:Nitric Oxide / Q000378:metabolism*; D019089:Stem Cell Factor / Q000494:pharmacology
D001565:Benzoates; D007093:Imidazoles; D007377:Interleukin-3; D015850:Interleukin-6; D019089:Stem Cell Factor; C079393:1,3-dihydroxy-4,4,5,5-tetramethyl-2-(4-carboxyphenyl)tetrahydroimidazole; D009569:Nitric Oxide