Mouse procathepsin E gene: molecular organisation and chromosomal localisation.
A 15.6 kb genomic clone encompassing the mouse procathepsin E gene was isolated and mapped. Sequencing revealed that the gene consists of nine exons followed by a polyadenylation signal at the 3'-end. The 5'-flanking region appears to be a TATA-less promoter but contains a nucleotide sequence that matches perfectly with the consensus motif of an initiator element [S.T. Smale, Biochim. Biophys. Acta 1351 (1997) 73-88.] to direct accurate initiation of transcription by RNA polymerase. This overlaps the site that was determined for the start of transcription. The absence of features considered typical of TATA-box regulated or housekeeping types of genes is consistent with the low levels of procathepsin E gene expression that are normally observed and might imply a unique sensitivity to or requirement for tissue-specific transcription factors that would account for the sporadic distribution of this aspartic proteinase in cells and tissues. The single copy of the procathepsin E gene was located on chromosome 1, near to that of mouse prorenin, a closely related aspartic proteinase involved in blood pressure regulation.
Tatnell
P J
PJ
School of Molecular and Medical Biosciences, University of Wales, Cardiff, P.O. Box 911, Cardiff CF1 3US, UK.
Roth
W
W
Deussing
J
J
Peters
C
C
Kay
J
J
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D020059:Cathepsin E; D002403:Cathepsins / Q000235:genetics*; D004247:DNA; D004792:Enzyme Precursors / Q000235:genetics*; D005091:Exons; D007438:Introns; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D008969:Molecular Sequence Data; D015183:Restriction Mapping; D014158:Transcription, Genetic
D004792:Enzyme Precursors; D004247:DNA; D002403:Cathepsins; C067581:procathepsin E; D020059:Cathepsin E