Production and secretion of endothelin-1 by cultured choroid plexus carcinoma cells.
Choroid plexus carcinoma is a rare neoplasm derived from the epithelium of the choroid plexus. The production and secretion of endothelin-1 (ET-1) by cultured human choroid plexus carcinoma cells were studied by radioimmunoassay and Northern blot analysis. Immunoreactive (IR)-ET was detected in the culture medium (2.78 +/- 0.12 fmol/10(5) cells/24 h; n = 5; mean +/- SEM) but not in the unconditioned medium. Reverse-phase high-performance liquid chromatography of the extract of the culture medium showed a single peak eluting in the position of ET-1. Treatment with tumor necrosis factor-alpha (TNF-alpha), interleukin-1 beta (IL-1 beta) or a combination of interferon-gamma (IFN-gamma), TNF-alpha, and IL-1 beta caused significant increases in the IR-ET levels in the culture medium. Northern blot analysis of total RNA showed the expression of ET-1 mRNA in choroid plexus carcinoma cells. The expression levels of ET-1 mRNA were increased by treatment with a combination of IFN-gamma, TNF-alpha, and IL-1 beta. The present study has shown the production and secretion of ET-1 by cultured human choroid plexus carcinoma cells and suggests the possibility that ET-1 formation is related to the pathophysiology of this tumor.
Takahashi
K
K
Department of Molecular Biology and Applied Physiology, Tohoku University School of Medicine, Sendai, Japan.
Hara
E
E
Murakami
O
O
Totsune
K
K
Sone
M
M
Satoh
F
F
Kumabe
T
T
Tominaga
T
T
Kayama
T
T
Yoshimoto
T
T
Shibahara
S
S
D015152:Blotting, Northern; D015740:Calcitonin Gene-Related Peptide / Q000378:metabolism; D002277:Carcinoma / Q000378:metabolism* / Q000473:pathology; D016545:Choroid Plexus Neoplasms / Q000378:metabolism* / Q000473:pathology; D002851:Chromatography, High Pressure Liquid; D016207:Cytokines / Q000378:metabolism; D019332:Endothelin-1 / Q000096:biosynthesis* / Q000378:metabolism; D006801:Humans; D009478:Neuropeptide Y / Q000378:metabolism; D011863:Radioimmunoassay; D014407:Tumor Cells, Cultured
D016207:Cytokines; D019332:Endothelin-1; D009478:Neuropeptide Y; D015740:Calcitonin Gene-Related Peptide