A monoclonal antibody-based enzyme immunoassay for the measurement of native and glycated apolipoprotein B-containing particles.
We describe the development of sandwich enzyme-linked immunosorbent assays designed to measure native and glycated apolipoprotein B containing particles in plasma. The assays utilize monoclonal antibodies anti native or glycated apo B-LDL for coating and a polyclonal anti apoB-LDL-peroxidase conjugate as the detecting antibody. The method is specific, sensitive and precise. The intra assay coefficient of variation for the plasma native and glycated apolipoprotein B-containing particles was determine to be 7.8% and 7.5%, respectively. The method described can provide specific and reproducible determinations of apoB and glycated-apoB containing particles in plasma; it will be of great interest in the evaluation of atherosclerotic risk in dyslipoproteinemic states in diabetic and non-diabetic subjects.
Calvo
C
C
Departamento de Bioquímica Clinicae Immunología, Facultad de Farmacia, Universidad de Concepción, Chile.
Bustos
P
P
Giraudo
P
P
Ulloa
N
N
Sepulveda
J
J
Verdugo
C
C
Toledo
L
L
D000911:Antibodies, Monoclonal; D001055:Apolipoproteins B / Q000097:blood*; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D006031:Glycosylation; D006801:Humans; D015203:Reproducibility of Results
D000911:Antibodies, Monoclonal; D001055:Apolipoproteins B