Poly(L-lysine)-graft-dextran copolymer is a novel stabilizer of triplex DNA(II): potassium-insensitive triplex formation.
Triple helix formation involving guanine-rich oligonucleotides is inhibited by physiological concentrations of potassium (K+). Using in vitro electrophoretic mobility shift assay (EMSA), we show that PLL-g-dex copolymer significantly stabilizes triplex structure at physiological levels of K+ that greatly inhibited triplex formation with a unmodified, guanine-rich oligonucleotide.
Ferdous
A
A
Department of Biomolecular Engineering, Tokyo Institute of Technology, Yokohama, Japan.
Watanabe
H
H
Maruyama
A
A
Akaike
T
T
D001483:Base Sequence; D004247:DNA / Q000737:chemistry*; D003911:Dextrans / Q000737:chemistry*; D004355:Drug Stability; D015394:Molecular Structure; D009690:Nucleic Acid Conformation; D009838:Oligodeoxyribonucleotides / Q000737:chemistry*; D011107:Polylysine / Q000031:analogs & derivatives* / Q000737:chemistry; D011188:Potassium
D003911:Dextrans; D009838:Oligodeoxyribonucleotides; C113090:poly(lysine)-graft-dextran; D011107:Polylysine; D004247:DNA; D011188:Potassium