title: Single-step purification of T7 RNA polymerase with a 6-histidine tag.
authors.affiliation: Dept. Molecular Information Processing, Institute of Molecular Biotechnology, Jena, Germany. ellinger@imb-jena.de
authors.forename: T
authors.initials: T
authors.lastname: Ellinger
authors.forename: R
authors.initials: R
authors.lastname: Ehricht
mesh_terms: D017123:Bacteriophage T7 / Q000201:enzymology* / Q000235:genetics*; D012321:DNA-Directed RNA Polymerases / Q000235:genetics* / Q000302:isolation & purification*; D004591:Electrophoresis, Polyacrylamide Gel; D006639:Histidine / Q000235:genetics*; D015202:Protein Engineering / Q000379:methods; D011993:Recombinant Fusion Proteins / Q000302:isolation & purification*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
chemical_list: D011993:Recombinant Fusion Proteins; D006639:Histidine; D012321:DNA-Directed RNA Polymerases