[Transplantation of fetal dopaminergic cells simultaneously to the corpus striatum and pars reticularis of the substantia nigra in hemi-parkinsonian rats].
BACKGROUND
Transplantation of foetal dopaminergic cells has been extensively used as restorative treatment for Parkinson's disease.


OBJECTIVE
This study was carried out to determine the survival, modifications in rotatory activity induced by D-amphetamine and total content of dopamine in the striatal and nigra regions of hemiparkinsonian rats which had had foetal mesencephalic cells simultaneously transplanted to the striatum and pars reticularis of the substancia nigra.


METHODS
The study was done using adult male Wistar rats weighing 200-250 gms. The following experimental groups were formed, depending on the site of transplant: St: transplant to striatum (n = 2); SNr: transplant to SNr (n = 20), ST + Snr; transplant to striatum and SNr simultaneously n = 20; and control (lesion with no transplant) n = 20. We studied the rotatory activity induced by D-amphetamine 1, 2, 3 and 6 months after transplantation. After this time the rats were deeply anaesthetized and randomly allocated for morphological study or biochemical determination of the total dopamine content in the St and SNr using the HPLC technique.


RESULTS
Study of conduct showed no significant differences in rotatory activity induced by D-amphetamine between the groups with intrastriatal transplants, but there was a difference between these and the SNr and control groups. Biochemical analysis showed that striatal DA content was significantly greater in the ST for the groups with intrastriatal transplants. The content of substancia nigra DA was significantly greater in the SNr of the ST + SNr group, followed by the ST group. Morphometric study showed differences, which were not significant, between ST transplanted animals and significant differences between the SNr transplanted group with a significant increase in survival of the SNr of the ST + SNr group.


CONCLUSIONS
These results suggest a positive effect due to intrastriatal transplants compared to survival following intranigral transplants.
Blanco
L
L
Grupo de Trastornos del Movimiento y EP, Centro Internacional de Restauración Neurológica, CIREN, La Habana, Cuba.
Pavón
N
N
Alvaŕez
P
P
Díaz
C
C
Castellano
O
O
Castillo
L
L
de la Cuétara
K
K
Macías
R
R
D018663:Adrenergic Agents / Q000493:pharmacokinetics*; D000704:Analysis of Variance; D000818:Animals; D002452:Cell Count; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D003342:Corpus Striatum / Q000737:chemistry* / Q000378:metabolism / Q000601:surgery*; D004298:Dopamine / Q000032:analysis* / Q000502:physiology*; D016332:Fetal Tissue Transplantation / Q000379:methods*; D007839:Functional Laterality; D008297:Male; D008636:Mesencephalon / Q000166:cytology / Q000196:embryology / Q000637:transplantation; D016627:Oxidopamine / Q000493:pharmacokinetics; D010300:Parkinson Disease / Q000601:surgery*; D051381:Rats; D017208:Rats, Wistar; D013378:Substantia Nigra / Q000737:chemistry* / Q000378:metabolism / Q000601:surgery*; D013997:Time Factors
D018663:Adrenergic Agents; D016627:Oxidopamine; D004298:Dopamine
Trasplante de células dopaminérgicas fetales simultáneamente en striatum y substantia nigra pars reticulata en ratas hemiparkinsonianas.