title: Escherichia coli ribosomal protein L3 stimulates the helicase activity of the Bacillus stearothermophilus PcrA helicase.
abstract: Escherichia coli ribosomal protein L3 stimulates the in vitro helicase activity of Bacillus stearothermophilus PcrA helicase upon a variety of different substrates. L3 has no intrinsic helicase or ATPase activity nor is it able to stimulate the ATPase activity of PcrA. Gel mobility shift assays revealed that the affinity of PcrA for a variety of different DNA species (single-stranded, nicked and 3'-tailed) was enhanced in the presence of L3. We suggest that the stimulatory effect of L3 upon the helicase activity of PcrA is mediated via a protein-protein interaction which promotes cooperative binding of PcrA to its DNA substrate. This activity of L3 appears to be specific for PcrA helicase.
authors.affiliation: Sir William Dunn School of Pathology, University of Oxford, South Parks Road, Oxford OX1 3RE, UK.
authors.forename: P
authors.initials: P
authors.lastname: Soultanas
authors.forename: M S
authors.initials: MS
authors.lastname: Dillingham
authors.forename: D B
authors.initials: DB
authors.lastname: Wigley
mesh_terms: D000251:Adenosine Triphosphatases / Q000378:metabolism; D001426:Bacterial Proteins / Q000378:metabolism*; D004265:DNA Helicases / Q000378:metabolism*; D004277:DNA, Single-Stranded / Q000378:metabolism; D004279:DNA, Viral / Q000378:metabolism; D004268:DNA-Binding Proteins / Q000378:metabolism; D004926:Escherichia coli / Q000737:chemistry*; D001411:Geobacillus stearothermophilus / Q000201:enzymology*; D011485:Protein Binding; D000090822:Ribosomal Protein L3; D012269:Ribosomal Proteins / Q000302:isolation & purification / Q000378:metabolism*; D014616:Vaccinia virus; D014758:Viral Core Proteins / Q000378:metabolism
chemical_list: D001426:Bacterial Proteins; D004277:DNA, Single-Stranded; D004279:DNA, Viral; D004268:DNA-Binding Proteins; D000090822:Ribosomal Protein L3; D012269:Ribosomal Proteins; D014758:Viral Core Proteins; C084217:pcrA protein, Bacteria; D000251:Adenosine Triphosphatases; D004265:DNA Helicases