Neither respiration nor cytochrome c oxidase affects mitochondrial morphology in Saccharomyces cerevisiae.
Previous studies have reported that mitochondrial morphology and volume in yeast cells are linked to cellular respiratory capacity. These studies revealed that mitochondrial morphology in glucose-repressed or anaerobically grown cells, which lack or have reduced levels of respiration, is different from that in fully respiring cells. Although both oxygen deprivation and glucose repression decrease the levels of respiratory chain proteins, they decrease the expression of many non-mitochondrial proteins as well, making it difficult to determine whether it is a defect in respiration or something else that effects mitochondrial morphology. To determine whether mitochondrial morphology is dependent on respiration per se, we used a strain with a null mutation in PET100, a nuclear gene that is specifically required for the assembly of cytochrome c oxidase. Although this strain lacks respiration, the mitochondrial morphology and volumes are both comparable to those found in its respiration-proficient parent. These findings indicate that respiration is not involved in the establishment or maintenance of yeast mitochondrial morphology, and that the previously observed effects of oxygen availability and glucose repression on mitochondrial morphology are not exerted through the respiratory chain. By applying the principle of symmorphosis to these findings, we conclude that the shape and size of the mitochondrial reticulum found in respiring yeast cells is maintained for reasons other than respiration.
Church
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Department of Molecular, Cellular, and Developmental Biology, University of Colorado, Boulder, CO 80309-0347, USA.
Poyton
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D002232:Carbocyanines; D003576:Electron Transport Complex IV / Q000378:metabolism*; D005456:Fluorescent Dyes; D018613:Microscopy, Confocal; D008854:Microscopy, Electron; D008928:Mitochondria / Q000648:ultrastructure*; D010101:Oxygen Consumption; D010641:Phenotype; D011726:Pyridinium Compounds; D012441:Saccharomyces cerevisiae / Q000648:ultrastructure*; D013053:Spectrophotometry
D002232:Carbocyanines; D005456:Fluorescent Dyes; D011726:Pyridinium Compounds; C011786:2-(4-(dimethylamino)styryl)-1-methylpyridinium; C007392:3,3'-dihexyl-2,2'-oxacarbocyanine; D003576:Electron Transport Complex IV