title: In vivo model for assessing the functional efficacy of endodontic filling materials and techniques.
abstract: Endodontic fillings were challenged with bacterial ingress in mandibular premolars of 4 beagle dogs. Groups 1, 2, and 3 (n = 9), had canals filled with gutta-percha and sealer, gutta-percha alone, and sealer alone, respectively. After 2 wk, pulp chambers were inoculated with plaque. Group 4 (n = 9) and group 5 (n = 5) had canals either filled as in groups 1 to 3 or unfilled, respectively, but not inoculated. Group 6 (n = 5) had canals unfilled and inoculated. Teeth were radiographed periodically for 14 wk, dogs terminated, and jaw blocks retrieved and processed for light microscopic examination. Rarefying osteitis appeared in group 6 at 3 wk and in groups 2, 3, and 5 at 11 wk. Periradicular inflammation was none, mild, or severe. Occurrence of severe inflammation in groups 1 to 6 was 0, 11%, 33%, 0, 60%, and 100%, respectively. Groups 1 to 3 combined differed significantly from group 4 (repeated-measures ANOVA, p < 0.05). This model could be used to assess the functional efficacy of endodontic fillings in vivo.
authors.affiliation: Department of Endodontics, University of Toronto Faculty of Dentistry, Ontario, Canada.
authors.forename: S
authors.initials: S
authors.lastname: Friedman
authors.forename: C D
authors.initials: CD
authors.lastname: Torneck
authors.forename: R
authors.initials: R
authors.lastname: Komorowski
authors.forename: Z
authors.initials: Z
authors.lastname: Ouzounian
authors.forename: P
authors.initials: P
authors.lastname: Syrtash
authors.forename: A
authors.initials: A
authors.lastname: Kaufman
mesh_terms: D000818:Animals; D001424:Bacterial Infections / Q000209:etiology / Q000382:microbiology / Q000628:therapy*; D001641:Bicuspid; D003786:Dental Pulp Cavity / Q000382:microbiology; D003788:Dental Pulp Diseases / Q000209:etiology / Q000382:microbiology / Q000628:therapy*; D004195:Disease Models, Animal; D004285:Dogs; D005069:Evaluation Studies as Topic; D008297:Male; D008334:Mandible; D010000:Osteitis / Q000209:etiology / Q000382:microbiology / Q000628:therapy; D010485:Periapical Periodontitis / Q000209:etiology / Q000382:microbiology / Q000628:therapy*; D012387:Root Canal Filling Materials / Q000627:therapeutic use*; D012390:Root Canal Therapy / Q000379:methods*; D013997:Time Factors
chemical_list: D012387:Root Canal Filling Materials