Genome structure of pTi-SAKURA (I): strategy for DNA sequencing of a Japanese cherry-Ti plasmid.
We isolated a plasmid from a bacterium Agrobacterium tumefaciens, which had been found in a crown gall tumor on a Japanese cherry tree SAKURA and designated it pTi-SAKURA. For complete DNA sequencing, we constructed a DNA library in lambda phage vector and developed a sequencing method by primer walking with long PCR and a PCR subcloning technique for long insert DNA.
Hattori
Y
Y
Department of Biological Science, Faculty of Science, Hiroshima University, Japan.
Suzuki
K
K
Uraji
M
M
Ohta
N
N
Katoh
A
A
Yoshida
K
K
D016960:Agrobacterium tumefaciens / Q000235:genetics*; D010582:Bacteriophage lambda; D003001:Cloning, Molecular; D017931:DNA Primers; D015723:Gene Library; D005822:Genetic Vectors; D016680:Genome, Bacterial; D007564:Japan; D010941:Plant Tumors; D010957:Plasmids; D016133:Polymerase Chain Reaction; D014197:Trees / Q000382:microbiology*
D017931:DNA Primers