DNA sequence dependent binding modes of bis(vinylpyridinium)benzene derivatives.
The DNA binding selectivity of new dicationic ligands based on the bis(vinylpyridinium)benzene unit has been investigated by means of UV-Vis absorption spectroscopy. From the experimental results it is concluded that these extended pi-electron bridged viologens have relatively high affinity to AT base pair sequences whereas the binding to GC pairs is about 10 times lower, and binding affinity depends on minor variation in the ligand structure. Linear type ligand exhibits two binding mode interaction, intercalation at high dye concentration which undergoes switching to groove binding at low ligand concentration.
Juskowiak
B
B
Department of Chemical Systems and Engineering, Graduate School of Engineering, Kyushu University, Fukuoka, Japan.
Takenaka
S
S
Takagi
M
M
Kondo
H
H
D001482:Base Composition; D001483:Base Sequence; D001665:Binding Sites; D004247:DNA / Q000737:chemistry*; D007202:Indicators and Reagents; D015394:Molecular Structure; D011067:Poly dA-dT / Q000737:chemistry*; D011089:Polydeoxyribonucleotides / Q000737:chemistry*; D011726:Pyridinium Compounds / Q000737:chemistry*; D013053:Spectrophotometry; D013343:Styrenes / Q000737:chemistry*
D007202:Indicators and Reagents; D011089:Polydeoxyribonucleotides; D011726:Pyridinium Compounds; D013343:Styrenes; D011067:Poly dA-dT; C019530:poly(dC-dG); D004247:DNA