Codon reading properties of tRNA variants substituted within the anticodon loop.
Effects of base substitution within tRNA anticodon loop on the codon reading activities were quantitatively analyzed with the use of a set of unmodified tRNA molecules with GGA anticodon. The first (position 32) and the last (position 38) nucleotides of the anticodon loop of the wild-type molecule was changed from C32A38 to U32A38, U32G38, and C32G38. The codon reading activities of these variants relative to that of the wild type molecule were measured in a cell-free translation system. The reading of both the UCU and UCC codons were lower in all the three variants than in the wild-type molecule.
Ouchi
R
R
Department of Industrial Chemistry, Faculty of Technology, Chiba Institute of Technology, Japan.
Takai
K
K
Yokoyama
S
S
Takaku
H
H
D000926:Anticodon / Q000737:chemistry / Q000235:genetics; D001483:Base Sequence; D003062:Codon / Q000737:chemistry / Q000235:genetics*; D004926:Escherichia coli / Q000235:genetics; D014644:Genetic Variation; D008958:Models, Molecular; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation; D017354:Point Mutation; D014176:Protein Biosynthesis; D012329:RNA, Bacterial / Q000737:chemistry / Q000235:genetics; D012333:RNA, Messenger / Q000096:biosynthesis / Q000737:chemistry; D012362:RNA, Transfer, Ser / Q000737:chemistry* / Q000235:genetics*; D013698:Templates, Genetic
D000926:Anticodon; D003062:Codon; D012329:RNA, Bacterial; D012333:RNA, Messenger; D012362:RNA, Transfer, Ser