Primary structure of ovine fibroblast growth factor-1 deduced by protein and cDNA analysis.
The amino acid sequence of full-length ovine fibroblast growth factor-1 (FGF-1) was determined by a combination of protein and cDNA sequencing. FGF-1 cDNA analysis indicated that ovine kidney cells express mRNAs encoding both full-length FGF-1 and a truncated FGF-1 variant. An overall comparison of the ovine FGF-1 primary sequence to the eight species studied to date revealed a high degree of conservation, with ovine FGF-1 sharing 90 and 95% sequence identity with human FGF-1 and bovine FGF-1, respectively. Additionally, the FGF-1 proteins from the various species have conserved cysteine residues at positions 30 and 97 and contain acetylated amino-terminal alanine residues. Mass spectrometry analysis confirmed that the blocking group of ovine FGF-1 is also consistent with that of an acetyl-moiety. In contrast to the other FGF-1 proteins, the 154 residue primary sequence of ovine FGF-1 contains three unique amino acid differences: Arg9, Arg44, and Ile123. Ovine FGF-1, unlike human FGF-1, is a potent mitogenic factor for NIH 3T3 fibroblasts in the absence of heparin. In the presence of exogenous heparin, the mitogenic activity of ovine FGF-1 is potentiated slightly.
Grieb
T W
TW
Department of Tissue Biology, Holland Laboratory, American Red Cross, Rockville, Maryland 20855, USA.
Ring
M
M
Brown
E
E
Palmer
C
C
Belle
N
N
Donjerkovic
D
D
Chang
H
H
Yun
J
J
Subramanian
R
R
Forozan
F
F
Guo
Y
Y
Vertes
A
A
Winkles
J A
JA
Burgess
W H
WH
D016475:3T3 Cells; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002417:Cattle; D002455:Cell Division / Q000187:drug effects; D017124:Conserved Sequence; D017931:DNA Primers / Q000235:genetics; D018076:DNA, Complementary / Q000235:genetics*; D016220:Fibroblast Growth Factor 1 / Q000737:chemistry / Q000235:genetics* / Q000494:pharmacology; D015870:Gene Expression; D006493:Heparin / Q000494:pharmacology; D006801:Humans; D007668:Kidney / Q000378:metabolism; D051379:Mice; D008934:Mitogens / Q000737:chemistry / Q000494:pharmacology; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D017386:Sequence Homology, Amino Acid; D012756:Sheep; D013045:Species Specificity; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
D017931:DNA Primers; D018076:DNA, Complementary; D008934:Mitogens; D012333:RNA, Messenger; D016220:Fibroblast Growth Factor 1; D006493:Heparin