title: The in vivo effect of isocyanate-induced asthma on basophil histamine release.
abstract: In vitro studies have suggested that basophils are involved in the asthmatic response to isocyanate. Classified by the type of airway response, three groups of patients exposed to isocyanate were studied: 1) nonreactive (n=3), 2) immediate responders (n=4), and 3) late responders (n=4). Basophil histamine release to concanavalin A (conA), FMLP, and anti-IgE was evaluated before and during the airway response. Histamine release significantly (P=0.009 for conA and 0.03 for anti-IgE) increased after exposure to isocyanate only in the group which had late bronchial response. Additionally, good correlation was found between baseline FEV1/FVC% and degree of histamine release of basophils stimulated with conA. These findings support a role for basophils in the late asthmatic response to isocyanate.
authors.affiliation: Institute of Pulmonary and Allergy Diseases, Tel Aviv Sourasky Medical Center and Sackler Faculty of Medicine, Tel Aviv University, Israel.
authors.forename: S
authors.initials: S
authors.lastname: Kivity
authors.forename: V
authors.initials: V
authors.lastname: Veintrov
authors.forename: E
authors.initials: E
authors.lastname: Fireman
authors.forename: O
authors.initials: O
authors.lastname: Agami
authors.forename: Y
authors.initials: Y
authors.lastname: Lerman
mesh_terms: D000328:Adult; D000368:Aged; D001249:Asthma / Q000139:chemically induced* / Q000378:metabolism / Q000503:physiopathology; D001491:Basophils / Q000378:metabolism*; D005541:Forced Expiratory Volume; D006636:Histamine Release; D006801:Humans; D008297:Male; D008875:Middle Aged; D014051:Toluene 2,4-Diisocyanate / Q000633:toxicity*
chemical_list: D014051:Toluene 2,4-Diisocyanate