Indomethacin inhibits delayed DNA fragmentation of hippocampal CA1 pyramidal neurons after transient forebrain ischemia in gerbils.
Recent studies have shown a close association between reactive oxygen species and DNA fragmentation and that delayed neuronal death after transient forebrain ischemia manifests as DNA fragmentation-like apoptosis. We examined the effect of indomethacin on ischemic-induced delayed hippocampal neuronal death in gerbils using the TUNEL staining method, since indomethacin is neuroprotective in a variety of degenerative processes, such as Alzheimer's disease. Indomethacin (5 mg/kg, i.p.), administered 5 min before the ischemic insult, significantly decreased the number of TUNEL-positive hippocampal CA1 pyramidal neurons and delayed the appearance of TUNEL-positive neurons. Our results indicate that indomethacin is effective in inhibiting DNA fragmentation after transient forebrain ischemia.
Kondo
F
F
Department of Neuroscience, Institute of Molecular and Cellular Medicine, Okayama University Medical School, Okayama 700-8558, Japan. kondor@cc.okayama-u.ac.up
Kondo
Y
Y
Gómez-Vargas
M
M
Ogawa
N
N
D000818:Animals; D016861:Cyclooxygenase Inhibitors / Q000627:therapeutic use*; D053938:DNA Fragmentation / Q000187:drug effects*; D004353:Drug Evaluation, Preclinical; D005821:Genetic Techniques; D005849:Gerbillinae; D007213:Indomethacin / Q000627:therapeutic use*; D002546:Ischemic Attack, Transient / Q000188:drug therapy* / Q000378:metabolism; D018696:Neuroprotective Agents / Q000627:therapeutic use*; D017966:Pyramidal Cells / Q000187:drug effects*
D016861:Cyclooxygenase Inhibitors; D018696:Neuroprotective Agents; D007213:Indomethacin