Recombinant antigens for specific and sensitive serodiagnosis of Latin American tegumentary leishmaniasis.
The diagnostic potential of recombinant leishmanial antigens for Latin American tegumentary leishmaniasis (LATL) was examined. Two Leishmania (Viannia) peruviana recombinant proteins, T26-U2 and T26-U4, were assessed by their reactivity to detect specific anti-leishmanial antibodies. Seventy-eight individual sera from persons with LATL, 39 from those with other diseases, and 10 negative control sera were tested by Western blotting and enzyme-linked immunosorbent assay. The sensitivity of the test using T26-U2 plus T26-U4 was similar to that obtained with whole parasite extract (92%). However, the specificity obtained using both recombinant antigens (87%) was higher than that of the whole parasite extract (65%). All tests using recombinant proteins (T26-U2, T26-U2 plus T26-U4 or T26-U4) had a higher positive predictive value (89%, 92% and 98%, respectively) than the value obtained using total parasites (81%). Eleven Colombian sera were also tested, and the results indicated that T26-U2 plus T26-U4 could be used successfully in Peru and in other Latin American countries.
Montoya
Y
Y
Instituto de Medicina Tropical Alexander von Humboldt, Universidad Peruana Cayetano Heredia, Lima, Peru.
Leon
C
C
Talledo
M
M
Nolasco
O
O
Padilla
C
C
Muñoz-Najar
U
U
Barker
D C
DC
D000818:Animals; D000953:Antigens, Protozoan / Q000737:chemistry; D015153:Blotting, Western; D003105:Colombia; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D007891:Leishmania; D007896:Leishmaniasis / Q000175:diagnosis*; D010568:Peru; D011237:Predictive Value of Tests; D011994:Recombinant Proteins / Q000737:chemistry; D012680:Sensitivity and Specificity; D012698:Serologic Tests / Q000379:methods / Q000592:standards
D000953:Antigens, Protozoan; D011994:Recombinant Proteins