Activation of protein kinase C delta by the c-Abl tyrosine kinase in response to ionizing radiation.
The c-Abl protein tyrosine kinase is activated by ionizing radiation (IR) and certain other DNA-damaging agents. The present studies demonstrate that c-Abl associates constitutively with protein kinase C delta (PKCdelta). The results show that the SH3 domain of c-Abl interacts directly with PKCdelta. c-Abl phosphorylates and activates PKCdelta in vitro. We also show that IR treatment of cells is associated with c-Abl-dependent phosphorylation of PKCdelta and translocation of PKCdelta to the nucleus. These findings support a functional interaction between c-Abl and PKCdelta in the cellular response to genotoxic stress.
Yuan
Z M
ZM
Division of Cancer Pharmacology, Dana-Farber Cancer Institute, Harvard Medical School, Boston, Massachusetts 02115, USA.
Utsugisawa
T
T
Ishiko
T
T
Nakada
S
S
Huang
Y
Y
Kharbanda
S
S
Weichselbaum
R
R
Kufe
D
D
D002588:Cesium Radioisotopes; D004789:Enzyme Activation; D018922:HL-60 Cells; D006801:Humans; D007527:Isoenzymes / Q000276:immunology / Q000378:metabolism*; D010766:Phosphorylation; D011493:Protein Kinase C / Q000276:immunology / Q000378:metabolism*; D051745:Protein Kinase C-delta; D016315:Proto-Oncogene Proteins c-abl / Q000276:immunology / Q000378:metabolism* / Q000528:radiation effects; D011839:Radiation, Ionizing; D011993:Recombinant Fusion Proteins / Q000378:metabolism; D014407:Tumor Cells, Cultured
D002588:Cesium Radioisotopes; D007527:Isoenzymes; D011993:Recombinant Fusion Proteins; D016315:Proto-Oncogene Proteins c-abl; C497258:PRKCD protein, human; D011493:Protein Kinase C; D051745:Protein Kinase C-delta