Regulation of cell proliferation and urokinase plasminogen activation of human breast epithelial cells by carrageenans.
Carrageenans, a family of polysulphated carbohydrates, are able to inhibit the binding to cells of growth factors such as transforming growth factor 1 (TGF 1), fibroblast growth factor-2 (FGF-2) and platelet-derived growth factor (PDGF) and so modulate cell invasion and proliferation. We studied the effects of carrageenans on the proliferation and on the uPA/PAI-1 system in breast epithelial cells. Carrageenans were able to inhibit the proliferation of both normal breast epithelial cells (NBEC) and breast epithelial cancer cell lines (MDA-MB-231 and MCF-7) but could only inhibit the uPA activity in the MDA-MB-231 cells. Moreover, carrageenans inhibited FGF-2 binding in all three cell types, suggesting that they regulate cell proliferation and uPA/PAI-1 system through two distinct mechanisms. These molecules could be considered as potentially useful anti-cancer agents.
Lambrecht
V
V
Unite de Dynamique des Cellules Embryonnaires et Cancereuses (EA 1033), Centre de Biologie Cellulaire, Universite des Sciences et Technologies de Lille, 59655 Villeneuve d'Ascq Cedex, France.
Boilly
B
B
Le Bourhis
X
X
D000925:Anticoagulants / Q000008:administration & dosage / Q000494:pharmacology; D001940:Breast / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism; D002351:Carrageenan / Q000008:administration & dosage / Q000494:pharmacology*; D002455:Cell Division / Q000187:drug effects; D004247:DNA / Q000187:drug effects / Q000378:metabolism; D004847:Epithelial Cells / Q000187:drug effects* / Q000378:metabolism; D005079:Excipients / Q000008:administration & dosage / Q000494:pharmacology*; D005260:Female; D016222:Fibroblast Growth Factor 2 / Q000187:drug effects / Q000378:metabolism; D006493:Heparin / Q000008:administration & dosage / Q000494:pharmacology; D006801:Humans; D007457:Iodine Radioisotopes; D010958:Plasminogen / Q000187:drug effects / Q000378:metabolism*; D017395:Plasminogen Activator Inhibitor 1 / Q000378:metabolism; D010960:Plasminogen Activators / Q000187:drug effects / Q000378:metabolism; D011485:Protein Binding / Q000187:drug effects; D014407:Tumor Cells, Cultured; D014568:Urokinase-Type Plasminogen Activator / Q000187:drug effects / Q000378:metabolism*
D000925:Anticoagulants; D005079:Excipients; D007457:Iodine Radioisotopes; D017395:Plasminogen Activator Inhibitor 1; D016222:Fibroblast Growth Factor 2; D002351:Carrageenan; D010958:Plasminogen; D006493:Heparin; D004247:DNA; D010960:Plasminogen Activators; D014568:Urokinase-Type Plasminogen Activator