title: The EGF receptor transmembrane domain: 2H NMR study of peptide phosphorylation effects in a bilayer environment.
abstract: Phosphorylation events are considered to be key control points in receptor tyrosine kinase function. We have used wide-line 2H NMR spectroscopy to look for physical effects of phosphorylating a threonine residue within the cytoplasmic domain of the human EGF receptor, as sensed at a distant site in the transmembrane portion. Modifications were made to Thr654 (a cytoplasmic residue suggested to be involved in regulation of EGF binding and of cytoplasmic domain function), and effects were sought at Ala623 (near the extracellular membrane surface but within the membrane-spanning region). The study was carried out on synthetic peptides corresponding to the EGF receptor transmembrane domain plus 10 or 11 residues of the cytoplasmic domain, assembled into lipid bilayer membranes. Three peptides were compared that differed only at Thr654. This residue was alternately: nonphosphorylated but left as a (-)-charged C-terminus (-Thr654COO-), nonphosphorylated and with a neighboring amidated glycine residue as the C-terminus (-Thr654GlyCONH2), or phosphorylated and with a neighboring amidated glycine residue as the C-terminus (-Thr654PO4-GlyCONH2). Bilayer membranes were composed of 1-palmitoyl-2-oleoylphosphatidylcholine (POPC) or 2:1 POPC/cholesterol, containing 6 mol % peptide relative to phospholipid. The deuterated site, Ala623, was intrinsically conformationally sensitive; yet spatial orientation and motional order of the probe location were found not to be obviously influenced by phosphorylation.
authors.affiliation: Department of Biochemistry, University of Western Ontario, London, Canada.
authors.forename: D H
authors.initials: DH
authors.lastname: Jones
authors.forename: K R
authors.initials: KR
authors.lastname: Barber
authors.forename: C W
authors.initials: CW
authors.lastname: Grant
mesh_terms: D000595:Amino Acid Sequence; D002462:Cell Membrane / Q000737:chemistry; D003903:Deuterium; D066246:ErbB Receptors / Q000737:chemistry*; D008051:Lipid Bilayers / Q000737:chemistry*; D009682:Magnetic Resonance Spectroscopy; D008565:Membrane Proteins / Q000138:chemical synthesis / Q000737:chemistry*; D008969:Molecular Sequence Data; D010446:Peptide Fragments / Q000138:chemical synthesis / Q000737:chemistry; D010455:Peptides / Q000138:chemical synthesis / Q000737:chemistry*; D010713:Phosphatidylcholines; D010766:Phosphorylation; D017434:Protein Structure, Tertiary
chemical_list: D008051:Lipid Bilayers; D008565:Membrane Proteins; D010446:Peptide Fragments; D010455:Peptides; D010713:Phosphatidylcholines; D003903:Deuterium; D066246:ErbB Receptors; C028694:1-palmitoyl-2-oleoylphosphatidylcholine