A PCR fingerprinting technique to distinguish isolates of Lactococcus lactis.
A fingerprinting technique similar to repetitive extragenic palindromic PCR was developed to identify strains of Lactococcus lactis. The method distinguishes closely related strains and discriminates among some with identical ldh sequences. The fingerprinting primer LL-Rep1 complements a moderately repeated sequence found in low G + C Gram-positive bacteria and may therefore prove useful for discriminating among strains of other low G + C Gram-positive species.
Urbach
E
E
Department of Microbiology, Oregon State University, Corvallis 97331, USA. urbache@bcc.orst.edu
Schindler
C
C
Giovannoni
S J
SJ
D001482:Base Composition; D016172:DNA Fingerprinting / Q000379:methods*; D017931:DNA Primers; D004269:DNA, Bacterial / Q000032:analysis* / Q000737:chemistry; D006094:Gram-Positive Bacteria / Q000235:genetics; D007770:L-Lactate Dehydrogenase / Q000235:genetics*; D013294:Lactococcus lactis / Q000235:genetics*; D012091:Repetitive Sequences, Nucleic Acid / Q000235:genetics
D017931:DNA Primers; D004269:DNA, Bacterial; D007770:L-Lactate Dehydrogenase