title: Sequence-selective RNA scission by oligoamine--DNA conjugates (1).
abstract: Ethylenediamine and diethylenetriamine, which are active for RNA hydrolysis, are attached to the 5'-ends of synthetic oligonucleotides by urethane linkages. The resultant artificial ribonucleases selectively hydrolyze the substrate RNA at the target phosphodiester linkage. Acid/base cooperation in the oligoamines for the catalysis is confirmed in terms of kinetic evidences.
authors.affiliation: Department of Chemistry and Biotechnology, Graduate School of Engineering, University of Tokyo, Japan.
authors.forename: A
authors.initials: A
authors.lastname: Kuzuya
authors.forename: Y
authors.initials: Y
authors.lastname: Azuma
authors.forename: T
authors.initials: T
authors.lastname: Inokawa
authors.forename: K
authors.initials: K
authors.lastname: Yoshinari
authors.forename: M
authors.initials: M
authors.lastname: Komiyama
mesh_terms: D001483:Base Sequence; D004247:DNA; D005029:Ethylenediamines; D006868:Hydrolysis; D007202:Indicators and Reagents; D009693:Nucleic Acid Hybridization; D009838:Oligodeoxyribonucleotides; D011073:Polyamines; D012313:RNA / Q000737:chemistry*
chemical_list: D005029:Ethylenediamines; D007202:Indicators and Reagents; D009838:Oligodeoxyribonucleotides; D011073:Polyamines; C005391:diethylenetriamine; C031234:ethylenediamine; D012313:RNA; D004247:DNA