title: Cloning and characterization of a novel class I cytokine receptor.
abstract: The human gp130 cDNA sequence was used as a query to search an expressed sequence tag database (dbEST) to identify cDNA sequences with similarity to the cytokine class I receptor family. A novel class I cytokine receptor was identified in a human infant brain cDNA library and was named WSX-1. Full-length cDNA sequences for human and murine WSX-1 were isolated and characterized. The WSX-1 cDNA encodes a 636 amino acid transmembrane protein with an extracellular domain of 482 amino acids and a cytoplasmic domain of 96 amino acids. The structure of the WSX-1 protein most closely resembles that of gp130. Northern blot analysis indicates high levels of expression in thymus, spleen, lymph node, and peripheral blood leukocytes, suggesting a role for WSX-1 in modulation of the immune response.
authors.affiliation: ZymoGenetics Inc., Seattle, Washington 98102, USA.
authors.forename: C A
authors.initials: CA
authors.lastname: Sprecher
authors.forename: F J
authors.initials: FJ
authors.lastname: Grant
authors.forename: J W
authors.initials: JW
authors.lastname: Baumgartner
authors.forename: S R
authors.initials: SR
authors.lastname: Presnell
authors.forename: S K
authors.initials: SK
authors.lastname: Schrader
authors.forename: T
authors.initials: T
authors.lastname: Yamagiwa
authors.forename: T E
authors.initials: TE
authors.lastname: Whitmore
authors.forename: P J
authors.initials: PJ
authors.lastname: O'Hara
authors.forename: D F
authors.initials: DF
authors.lastname: Foster
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D001921:Brain / Q000378:metabolism; D002874:Chromosome Mapping; D003001:Cloning, Molecular; D017124:Conserved Sequence; D017931:DNA Primers / Q000235:genetics; D018076:DNA, Complementary / Q000235:genetics; D006801:Humans; D006822:Hybrid Cells; D007223:Infant; D008024:Ligands; D051379:Mice; D008969:Molecular Sequence Data; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D018121:Receptors, Cytokine / Q000145:classification* / Q000235:genetics* / Q000378:metabolism; D018123:Receptors, Interleukin; D017386:Sequence Homology, Amino Acid; D015398:Signal Transduction; D014018:Tissue Distribution
chemical_list: D017931:DNA Primers; D018076:DNA, Complementary; C112441:IL27RA protein, human; C484747:Il27ra protein, mouse; D008024:Ligands; D012333:RNA, Messenger; D018121:Receptors, Cytokine; D018123:Receptors, Interleukin