The non-peptide neuroprotective agents SR 57746A interacts with neurotrophin 3 to induce differentiation in the PC12 cell-line.
SR 57746A (1-(2beta-naphthylethyl)-4-(3-trifluoromethylphenyl)-1,2,5,6 -tetrahydropyridine hydrochloride) is a neuroprotective compound which potentiates nerve-growth factor (NGF)-induced differentiation in PC12 cells. We have evaluated the interaction of SR 57746A with the other members of the neurotrophin family in this cell-line. In contrast with NGF, neurotrophin-3 did not increase the differentiation of PC12 cells. However, the association of SR 57746A with neurotrophin-3 significantly increased neurite outgrowth. No significant activity on neurite outgrowth was observed with brain-derived neurotrophic factor or neurotrophin-4, either alone or combined with SR 57746A. These results indicate that as well as potentiating the effect of NGF SR 57746A enables neurotrophin-3, which alone is inactive, to increase the differentiation of these cells.
Fournier
J
J
Sanofi Recherche, Toulouse, France.
Boutot
G
G
Keane
P E
PE
Maffrand
J P
JP
Le Fur
G
G
Soubrié
P
P
D000818:Animals; D019208:Brain-Derived Neurotrophic Factor / Q000494:pharmacology; D002454:Cell Differentiation / Q000187:drug effects*; D016895:Culture Media, Serum-Free; D004357:Drug Synergism; D009281:Naphthalenes / Q000494:pharmacology*; D009414:Nerve Growth Factors / Q000494:pharmacology*; D016501:Neurites / Q000187:drug effects; D018696:Neuroprotective Agents / Q000494:pharmacology*; D020933:Neurotrophin 3; D016716:PC12 Cells; D011725:Pyridines / Q000494:pharmacology*; D051381:Rats; D017366:Serotonin Receptor Agonists / Q000494:pharmacology*
D019208:Brain-Derived Neurotrophic Factor; D016895:Culture Media, Serum-Free; D009281:Naphthalenes; D009414:Nerve Growth Factors; D018696:Neuroprotective Agents; D020933:Neurotrophin 3; D011725:Pyridines; D017366:Serotonin Receptor Agonists; C073922:neurotrophin 4; C462299:xaliproden