title: Relationship between ciprofloxacin, ofloxacin, levofloxacin, sparfloxacin and moxifloxacin (BAY 12-8039) MICs and mutations in grlA, grlB, gyrA and gyrB in 116 unrelated clinical isolates of Staphylococcus aureus.
abstract: The in-vitro activities of five fluoroquinolones were tested against 70 ciprofloxacin-resistant and 46 ciprofloxacin-susceptible unrelated isolates of Staphylococcus aureus. All 116 S. aureus isolates were studied for the presence of mutations in the grl and gyr loci. The order of efficacy of the fluoroquinolones tested, from least to most active, was: ciprofloxacin, ofloxacin, levofloxacin, sparfloxacin and moxifloxacin (BAY 12-8039), in response to all characterized mutations in grlA, grlB, gyrA and gyrB. Moxifloxacin was active against most S. aureus isolates tested (MIC90 = 1 mg/L for ciprofloxacin-resistant isolates) and was less influenced by known mutations.
authors.affiliation: Institute for Medical Microbiology and Virology, Heinrich-Heine University, Düsseldorf, Germany.
authors.forename: F J
authors.initials: FJ
authors.lastname: Schmitz
authors.forename: B
authors.initials: B
authors.lastname: Hofmann
authors.forename: B
authors.initials: B
authors.lastname: Hansen
authors.forename: S
authors.initials: S
authors.lastname: Scheuring
authors.forename: M
authors.initials: M
authors.lastname: Lückefahr
authors.forename: M
authors.initials: M
authors.lastname: Klootwijk
authors.forename: J
authors.initials: J
authors.lastname: Verhoef
authors.forename: A
authors.initials: A
authors.lastname: Fluit
authors.forename: H P
authors.initials: HP
authors.lastname: Heinz
authors.forename: K
authors.initials: K
authors.lastname: Köhrer
authors.forename: M E
authors.initials: ME
authors.lastname: Jones
mesh_terms: D000890:Anti-Infective Agents / Q000494:pharmacology*; D002939:Ciprofloxacin / Q000494:pharmacology; D027081:DNA Gyrase; D027101:DNA Topoisomerase IV; D004250:DNA Topoisomerases, Type II / Q000235:genetics; D004269:DNA, Bacterial / Q000032:analysis; D004352:Drug Resistance, Microbial; D006801:Humans; D008826:Microbial Sensitivity Tests; D009154:Mutation; D016133:Polymerase Chain Reaction; D013211:Staphylococcus aureus / Q000187:drug effects* / Q000235:genetics* / Q000302:isolation & purification
chemical_list: D000890:Anti-Infective Agents; D004269:DNA, Bacterial; D002939:Ciprofloxacin; D027101:DNA Topoisomerase IV; D027081:DNA Gyrase; D004250:DNA Topoisomerases, Type II