title: Glyoxalases activity during Bufo bufo embryo development.
abstract: In this work we have investigated the expression of glyoxalase I (GLO I) and glyoxalase II (GLO II) activities during Bufo bufo embryo development and in some tissues of both male and female adult animals, in order to study how they correlate with cell proliferation and differentiation. The results show that both the activities are expressed at significant levels from the earliest developmental stages, reaching the highest values at the end of embryonic development (stage 25). The GLO I/GLO II ratio is very high at the beginning of the development and then gradually decreases as the development goes on. These data emphasize the importance of GLO I activity in the phases in which elevated cell division is taking place. In the differentiated tissues, a peculiar sexual dimorphism in both GLO I and GLO II activities, with higher values in female than in male, was found. GLO I embryonic activity levels are comparable to those found in female differentiated tissues, but significantly higher than those detected in male differentiated tissues. On the contrary, the GLO II activities found in the adult tissues were always higher than those found in embryos. These results further support the idea that high GLO I/GLO II ratios are a characteristic of the proliferative status, which assures a good scavenging action against the potentially cytotoxic and cytostatic effect of methylglyoxal.
authors.affiliation: Dipartimento di Biologia di Base ed Applicata, Università Di L'Aquila, Italy.
authors.forename: F
authors.initials: F
authors.lastname: Amicarelli
authors.forename: P
authors.initials: P
authors.lastname: Sacchetta
authors.forename: S
authors.initials: S
authors.lastname: Colafarina
authors.forename: S
authors.initials: S
authors.lastname: Angelucci
authors.forename: M
authors.initials: M
authors.lastname: Miranda
authors.forename: C
authors.initials: C
authors.lastname: Di Ilio
mesh_terms: D000818:Animals; D002023:Bufo bufo; D004625:Embryo, Nonmammalian / Q000201:enzymology; D047108:Embryonic Development; D005260:Female; D007791:Lactoylglutathione Lyase / Q000378:metabolism*; D008297:Male; D013869:Thiolester Hydrolases / Q000378:metabolism*; D000099301:Hydroxyacylglutathione Hydrolase
chemical_list: D007791:Lactoylglutathione Lyase; D013869:Thiolester Hydrolases; D000099301:Hydroxyacylglutathione Hydrolase