Comparison of the regulation of endothelin-2 and endothelin-converting enzyme-1 b [correction of beta] by forskolin and TNF-alpha in ACHN cells.
The effects on the expression and secretion of ET-2 of forskolin and tumor necrosis factor-alpha (TNF-alpha) were investigated using the human renal adenocarcinoma (ACHN) cell line. For comparison, changes in endothelin-converting enzyme-1 b (ECE-1 b) [corrected] mRNA were also determined. Treatment for 4 with TNF-alpha (3 ng/ml), forskolin (30 microM), or the combination caused significant increases in ET-2 release, TNF-alpha alone or in combination with forskolin increased ET-2 mRNA levels at 1 h and 2 h. After 4 h the expression of ET-2 mRNA was comparable to control levels. In contrast to ET-2, ECE-1 b [corrected] mRNA levels were increased by TNF-alpha only at 4 h. Forskolin increased expression of ET-2 mRNA at 1 and 4 but had no significant effect on expression of ECE-beta. These data suggest that expression of ET-2 and ECE-1 b [corrected] mRNA is regulated differently in ACHN cells.
Lambert
G L
GL
William Harvey Research Institute, St. Bartholomew's, Queen Mary and Westfield College, London, England.
Barker
S
S
Corder
R
R
D000230:Adenocarcinoma / Q000378:metabolism*; D016282:Aspartic Acid Endopeptidases / Q000378:metabolism*; D005576:Colforsin / Q000494:pharmacology*; D019333:Endothelin-2 / Q000378:metabolism*; D000072396:Endothelin-Converting Enzymes; D006801:Humans; D007680:Kidney Neoplasms / Q000378:metabolism*; D008666:Metalloendopeptidases / Q000378:metabolism*; D011489:Protein Denaturation; D012333:RNA, Messenger / Q000096:biosynthesis; D014407:Tumor Cells, Cultured; D014409:Tumor Necrosis Factor-alpha / Q000494:pharmacology*
D019333:Endothelin-2; D012333:RNA, Messenger; D014409:Tumor Necrosis Factor-alpha; D005576:Colforsin; D016282:Aspartic Acid Endopeptidases; D008666:Metalloendopeptidases; C000606923:ECE1 protein, human; D000072396:Endothelin-Converting Enzymes