Saccharomyces cerevisiae GPI10, the functional homologue of human PIG-B, is required for glycosylphosphatidylinositol-anchor synthesis.
An increasing number of plasma membrane proteins have been shown to be attached to the membrane via a glycosylphosphatidylinositol (GPI) moiety. All eukaryotes share a highly conserved GPI-core structure EthN-P-Man3-GlcN-PI, where EthN is ethanolamine. We have identified a protein encoded by the yeast open reading frame YGL142C that shares 33% identity with the human Pig-B protein. Deletion of this essential gene leads to a block in GPI anchor biosynthesis. We therefore named the gene GPI10. Gpi10p and Pig-B are functional homologues and the lethal deletion of GPI10 can be rescued by expression of the PIG-B cDNA. As found for PIG-B mutant cells, gpi10 deletant cells cannot attach the third mannose in an alpha-1,2 linkage to the GPI core-structure intermediate. Overexpression of GPI10 gives partial resistance to the GPI-synthesis inhibitor YW3548, suggesting that this gene product may affect the target of the inhibitor.
Sütterlin
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Biozentrum of the University of Basel, Klingelbergstrasse 70, CH-4056 Basel, Switzerland.
Escribano
M V
MV
Gerold
P
P
Maeda
Y
Y
Mazon
M J
MJ
Kinoshita
T
T
Schwarz
R T
RT
Riezman
H
H
D000595:Amino Acid Sequence; D002236:Carbohydrate Conformation; D002455:Cell Division / Q000235:genetics; D003001:Cloning, Molecular; D005656:Fungal Proteins / Q000737:chemistry*; D006017:Glycolipids / Q000737:chemistry; D017261:Glycosylphosphatidylinositols / Q000096:biosynthesis*; D006801:Humans; D007783:Lactones / Q000494:pharmacology; D008358:Mannose / Q000378:metabolism; D008364:Mannosyltransferases / Q000737:chemistry*; D008565:Membrane Proteins / Q000737:chemistry; D008969:Molecular Sequence Data; D012441:Saccharomyces cerevisiae / Q000201:enzymology*; D029701:Saccharomyces cerevisiae Proteins; D016415:Sequence Alignment; D017421:Sequence Analysis; D017384:Sequence Deletion / Q000235:genetics; D013729:Terpenes / Q000494:pharmacology
D005656:Fungal Proteins; D006017:Glycolipids; D017261:Glycosylphosphatidylinositols; D007783:Lactones; D008565:Membrane Proteins; D029701:Saccharomyces cerevisiae Proteins; D013729:Terpenes; C109532:YW 3548; C484448:GPI10 protein, S cerevisiae; D008364:Mannosyltransferases; C102168:PIGB protein, human; D008358:Mannose