Analysis of the promoter region of the murine complement factor H gene.
We have used the luciferase system to assay basal promoter activity of the murine factor H gene. Based on the results from luciferase assays with clones of 13 nested deletions, a 242-bp region that appeared to contain an enhancer element was subcloned upstream of a heterologous promoter and was shown to enhance transcription. A 26-bp fragment from this region was shifted in electrophoretic mobility assays, and this fragment contains a consensus sequence for the adenovirus major late transcription factor/upstream stimulatory factor (MLTF/USF). This fragment had enhancing activity in a minimal factor H promoter construct, demonstrating that it is a major enhancer of the factor H gene in murine liver cells.
Kain
S J
SJ
Department of Molecular Genetics and Microbiology, University of New Mexico School of Medicine, Albuquerque, NM 87131-5276, USA.
Maldonado
M J
MJ
Vik
D P
DP
D000818:Animals; D001483:Base Sequence; D001665:Binding Sites / Q000235:genetics; D002460:Cell Line; D003001:Cloning, Molecular; D017242:Complement Factor H / Q000235:genetics*; D016384:Consensus Sequence; D004247:DNA / Q000235:genetics / Q000378:metabolism; D004268:DNA-Binding Proteins; D004742:Enhancer Elements, Genetic; D008099:Liver / Q000378:metabolism; D008156:Luciferases / Q000235:genetics; D051379:Mice; D011401:Promoter Regions, Genetic; D017384:Sequence Deletion; D014157:Transcription Factors / Q000378:metabolism; D014162:Transfection; D051720:Upstream Stimulatory Factors
D004268:DNA-Binding Proteins; D014157:Transcription Factors; D051720:Upstream Stimulatory Factors; C497183:Usf1 protein, mouse; D017242:Complement Factor H; D004247:DNA; D008156:Luciferases