Novel CRE-binding proteins of 11-16 kDa bind to the LDH A-gene CRE in a sequence specific and hepatocyte-growth dependent manner in partially hepatectomized rat liver.
We examined cAMP response element (CRE)-binding proteins involved in lactate dehydrogenase A (LDHA)-gene transcription in rat liver after partial hepatectomy. Gel retardation and Southwestern blot assays showed that the CRE-binding activity of the 11-16 kDa novel proteins increased in accordance with increases in LDH A-mRNA in regenerating liver tissues, whereas that of the 43 kDa CREB did not. Using CRE-oligonucleotide affinity chromatography and reverse-phase HPLC, we purified four CRE-binding proteins of 11.2, 15.2, 15.8, and 16.3 kDa. N-terminal amino acid sequences of 15.2 and 16.3 kDa proteins revealed a high sequence homology to but were not identical with those of rat histone H2A.1 and H2B, respectively. CRE-bindings of these two proteins were highly specific, while those of histones H2A.1 and H2B were nonspecific as shown by competition-Southwestern blot and DNase I footprinting assays. Taking these data together, we suggest that the novel 11-16 kDa CRE-binding proteins are responsible for the cell growth-dependent inducibility of LDH A-gene transcription during liver regeneration.
Lee
M Y
MY
College of Pharmacy, Seoul National University, Korea.
Hwang
E S
ES
Lee
S K
SK
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D001665:Binding Sites / Q000235:genetics; D000242:Cyclic AMP / Q000378:metabolism; D017362:Cyclic AMP Response Element-Binding Protein / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*; D004247:DNA / Q000235:genetics* / Q000378:metabolism*; D015342:DNA Probes / Q000235:genetics; D006498:Hepatectomy; D006657:Histones / Q000235:genetics; D007527:Isoenzymes; D007770:L-Lactate Dehydrogenase / Q000235:genetics*; D008099:Liver / Q000378:metabolism*; D008115:Liver Regeneration / Q000235:genetics / Q000502:physiology; D008969:Molecular Sequence Data; D008970:Molecular Weight; D051381:Rats; D017207:Rats, Sprague-Dawley; D017386:Sequence Homology, Amino Acid
D017362:Cyclic AMP Response Element-Binding Protein; D015342:DNA Probes; D006657:Histones; D007527:Isoenzymes; D004247:DNA; D000242:Cyclic AMP; D007770:L-Lactate Dehydrogenase