Chemotactic factors in bronchial secretions of cystic fibrosis patients.
To understand chronic neutrophil attraction into cystic fibrosis airways, both global chemotactic activity and individual chemotactic factors were studied in bronchial secretions. Bronchial secretions of 8 cystic fibrosis patients, collected on the first day of admission for antibiotic treatment, showed a high chemotactic index (19.4 +/- 5.7, n = 8). Fractionation by gel filtration of bronchial secretions resulted in three chemotactic fractions. The first factor corresponded to interleukin-8, and the second activated neutrophils via the FMLP receptor. The third factor, which was of lower molecular weight, did not activate FMLP or leukotriene B4 receptors, and its nature is still under investigation. Treating patients with antibiotics reduced global chemotactic activity, mainly by reducing the activity due to stimulation of the FMLP receptor.
Dayer Pastore
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F
Department of Pediatrics and Pulmonary Division of the Department of Medicine, University Hospitals of Geneva, Switzerland. Fabienne.Dayer@hcuge.ch
Schlegel-Haueter
S E
SE
Belli
D C
DC
Rochat
T
T
Dudez
T S
TS
Suter
S
S
D000293:Adolescent; D000328:Adult; D000617:Aminoglycosides; D000900:Anti-Bacterial Agents / Q000627:therapeutic use; D001980:Bronchi / Q000378:metabolism; D001992:Bronchoalveolar Lavage Fluid / Q000737:chemistry*; D002511:Cephalosporins / Q000627:therapeutic use; D002630:Chemotactic Factors / Q000032:analysis*; D002634:Chemotaxis, Leukocyte; D002648:Child; D002850:Chromatography, Gel; D003550:Cystic Fibrosis / Q000150:complications / Q000503:physiopathology*; D004359:Drug Therapy, Combination / Q000627:therapeutic use; D006801:Humans; D008875:Middle Aged; D011552:Pseudomonas Infections / Q000150:complications* / Q000188:drug therapy; D013183:Sputum / Q000737:chemistry
D000617:Aminoglycosides; D000900:Anti-Bacterial Agents; D002511:Cephalosporins; D002630:Chemotactic Factors