Comparative protection against oxyradicals by three flavonoids on cultured endothelial cells.
Oxygen-derived free radicals are known to injure the endothelium of aorta in diverse disorders. In this study we compared the cytoprotective effects of three flavonoids against oxyradical damage to porcine aortic endothelial cells in vitro. Cultured porcine aortic endothelial cells were exposed to oxyradicals generated by xanthine oxidase--hypoxanthine (XO-HP). The cytoprotective activities of morin, quercetin, and catechin on these systems were compared using established morphologic criteria. The results in the XO-HP system showed that morin at 0.125, 0.25, and 0.5 mM delayed cell necrosis to 27.4 +/- 1.3, 46.8 +/- 1.8, and longer than 70 min, respectively, compared with 12.0 +/- 1.3 min in the control group. These degrees of protection were significantly stronger than those provided by quercetin and catechin at corresponding concentrations (p < 0.01). Morin and quercetin were moderate inhibitors of xanthine oxidase on the basis of the oxygen consumption rate, whereas catechin at the same concentrations had little inhibitory effect. The data from uric acid formation and cytochrome c reduction were consistent with the oxygen consumption measurement for the three flavonoids.
Zeng
L H
LH
Department of Clinical Biochemistry, University of Toronto, ON, Canada.
Wu
J
J
Fung
B
B
Tong
J H
JH
Mickle
D
D
Wu
T W
TW
D000818:Animals; D000975:Antioxidants / Q000494:pharmacology; D001011:Aorta; D002392:Catechin / Q000494:pharmacology; D002478:Cells, Cultured; D004730:Endothelium, Vascular / Q000187:drug effects* / Q000473:pathology; D005419:Flavonoids / Q000494:pharmacology*; D009336:Necrosis; D011794:Quercetin / Q000494:pharmacology; D017382:Reactive Oxygen Species; D013552:Swine; D014969:Xanthine Oxidase / Q000494:pharmacology; D047309:Flavones
D000975:Antioxidants; D002392:Catechin; D005419:Flavonoids; D011794:Quercetin; D017382:Reactive Oxygen Species; D014969:Xanthine Oxidase; C008548:morin; D047309:Flavones