[Effect of ionophore A23187 and verapamil on RNA and DNA polymerase activity in rat liver nuclei].
It was shown that verapamil and Ca(2+)-ionofor A23187 essentially inhibited the activity of RNA-polymerase in isolated nuclei of rat liver, while DNA-polymerasing activity was inhibited only after the addition of verapamil. These phenomena were not found after treatment of nuclei with 1% triton X-100. The inhibition of RNA synthesis was also found after the addition of Ca(2+)-ionofor A23187 but not verapamil to nuclei which envelope was disrupted by freezing-thawing procedure. The data obtained suggest that Ca(2+)-ionofor A23187 and verapamil affected RNA and DNA synthesis in isolated nuclei.
Kapralov
A A
AA
Research Institute of Physiology, Taras Shevchenko University, Kyiv, Ukraine.
D000818:Animals; D000001:Calcimycin / Q000494:pharmacology*; D002118:Calcium / Q000378:metabolism; D002121:Calcium Channel Blockers / Q000494:pharmacology*; D002467:Cell Nucleus / Q000187:drug effects / Q000201:enzymology; D004247:DNA / Q000096:biosynthesis; D012321:DNA-Directed RNA Polymerases / Q000037:antagonists & inhibitors*; D004791:Enzyme Inhibitors / Q000494:pharmacology*; D005260:Female; D066298:In Vitro Techniques; D007476:Ionophores / Q000494:pharmacology*; D008099:Liver / Q000166:cytology / Q000187:drug effects* / Q000201:enzymology; D019384:Nucleic Acid Synthesis Inhibitors; D017830:Octoxynol; D012313:RNA / Q000096:biosynthesis; D051381:Rats; D014700:Verapamil / Q000494:pharmacology*
D002121:Calcium Channel Blockers; D004791:Enzyme Inhibitors; D007476:Ionophores; D019384:Nucleic Acid Synthesis Inhibitors; D000001:Calcimycin; D012313:RNA; D017830:Octoxynol; D004247:DNA; D014700:Verapamil; D012321:DNA-Directed RNA Polymerases; D002118:Calcium
Vliianie ionofora A23187 i verapamila na RNK- i DNK-polimeraznuiu aktivnost' v iadrakh kletok pecheni krys.