TrkA receptors delay C6-2B glioma cell growth in rat striatum.
Nerve growth factor (NGF) acts as an anti-mitogenic factor in C6-2B glioma cells stably expressing TrkA (C6trk+). To study the effect of TrkA on cell growth in vivo, we grafted mock and C6trk+ cells into the striatum of ACI nude rats. Thy 1.1 and p75NTR immunohistochemistry revealed that wild type C6-2B cells formed a tumor mass in the striatum by 14 days. In contrast, C6trk+ transplanted rats did not show the presence of a significant tumor mass until 71 days. Analysis of this tumor showed that expression of TrkA was retained, but the synthesis of NGF was abolished. Our data encourage the speculation that expression of TrkA in glioblastoma in vivo will attenuate tumor progression.
Rabin
S J
SJ
Department of Cell Biology, Georgetown University, Washington, DC 20007, USA.
Tornatore
C
C
Baker-Cairns
B
B
Spiga
G
G
Mocchetti
I
I
D000818:Animals; D002455:Cell Division / Q000235:genetics; D003342:Corpus Striatum / Q000378:metabolism* / Q000473:pathology*; D005910:Glioma / Q000378:metabolism* / Q000473:pathology; D007150:Immunohistochemistry; D011518:Proto-Oncogene Proteins / Q000235:genetics / Q000502:physiology*; D051381:Rats; D011923:Rats, Nude; D020794:Receptor Protein-Tyrosine Kinases / Q000235:genetics / Q000502:physiology*; D020917:Receptor, trkA; D017475:Receptors, Nerve Growth Factor / Q000235:genetics / Q000502:physiology*; D014162:Transfection; D014407:Tumor Cells, Cultured
D011518:Proto-Oncogene Proteins; D017475:Receptors, Nerve Growth Factor; D020794:Receptor Protein-Tyrosine Kinases; D020917:Receptor, trkA