title: High-molecular-weight hyaluronan--a valuable tool in testing the antioxidative activity of amphiphilic drugs stobadine and vinpocetine.
abstract: The antioxidative activity of stobadine and vinpocetine was studied in vitro by measuring their inhibition effect on the depolymerization of the high-molecular-weight hyaluronan by hydroxyl radicals. The radicals were generated by the Cu(2+)-H2O2 system. Hyaluronan depolymerization was monitored by means of size exclusion chromatography. The antioxidative activity of stobadine and vinpocetine was compared to that of D-mannitol. A 50% inhibition of hyaluronan depolymerization was reached at stobadine and vinpocetine concentrations of 1.7 x 10(-6) and 3.0 x 10(-7) mol l-1, respectively, while a D-mannitol level of 2.6 x 10(-3) mol l-1 was needed to achieve the same inhibitory effect.
authors.affiliation: Research Institute of Rheumatic Diseases, Piest'any, Slovak Republic.
authors.forename: E
authors.initials: E
authors.lastname: Orviský
authors.forename: L
authors.initials: L
authors.lastname: Soltés
authors.forename: M
authors.initials: M
authors.lastname: Stancíková
mesh_terms: D000975:Antioxidants / Q000737:chemistry*; D002243:Carbolines / Q000737:chemistry*; D002850:Chromatography, Gel; D016166:Free Radical Scavengers; D006820:Hyaluronic Acid / Q000737:chemistry*; D008353:Mannitol / Q000737:chemistry; D008970:Molecular Weight; D014748:Vinca Alkaloids / Q000737:chemistry*
chemical_list: D000975:Antioxidants; D002243:Carbolines; D016166:Free Radical Scavengers; D014748:Vinca Alkaloids; D008353:Mannitol; C013983:vinpocetine; D006820:Hyaluronic Acid; C100106:dicarbine