Processing of vertebrate box C/D small nucleolar RNAs in plant cells.
The recent isolation of a number of plant box C/D small nucleolar (sno)RNAs demonstrates the conservation in plants of sequence and structural elements of processed box C/D snoRNAs. Boxes C and D, and terminal inverted repeats are known to be essential for accumulation and processing in vertebrates and yeast. Processing of vertebrate box C/D snoRNAs was examined by expression of various mouse hsc70 intron 5-U14 constructs in tobacco protoplasts. Full-length U14 and internally deleted U14 accumulated in the plant cells. Human U3 and U8 fragments, consistent with processing to internal box C/C' sequences, also accumulated in the plant cells. The similarity of processing behaviour of the vertebrate box C/D constructs in tobacco protoplasts and Xenopus oocytes suggests the mechanism of processing, involving recognition and association of proteins, is conserved in plants.
Leader
D J
DJ
Department of Cell and Molecular Genetics, Scottish Crop Research Institute, Dundee, UK.
Clark
G P
GP
Boag
J
J
Watters
J A
JA
Simpson
C G
CG
Watkins
N J
NJ
Maxwell
E S
ES
Brown
J W
JW
D000818:Animals; D001483:Base Sequence; D005260:Female; D006801:Humans; D066298:In Vitro Techniques; D007438:Introns; D051379:Mice; D009838:Oligodeoxyribonucleotides / Q000235:genetics; D009865:Oocytes / Q000378:metabolism; D030821:Plants, Genetically Modified; D010947:Plants, Toxic; D012323:RNA Processing, Post-Transcriptional; D012326:RNA Splicing; D012342:RNA, Small Nuclear / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D014026:Nicotiana / Q000235:genetics / Q000378:metabolism; D014981:Xenopus
D009838:Oligodeoxyribonucleotides; D012342:RNA, Small Nuclear