title: Importance of the N-glycan in the V3 loop of HIV-1 envelope protein for CXCR-4- but not CCR-5-dependent fusion.
abstract: The V3 region of HIV-1 envelope protein possesses a single N-linked sugar chain, which is conserved in most HIV-1 strains. We studied its role in the life cycle of HIV-1 strains with different co-receptor usage. Removal of the glycan appeared to cause a marked reduction of CXCR-4- but not CCR-5-dependent virus entry. A basic amino acid substitution at the 11th position of V3 markedly compensated for the removal of the N-glycan. These results indicate that the N-glycan plays an important role for CXCR-4-dependent virus entry and that this role is exerted in a particular context of the peptide backbone.
authors.affiliation: Department of Viral Infection, Institute of Medical Science, University of Tokyo, Japan.
authors.forename: E E
authors.initials: EE
authors.lastname: Nakayama
authors.forename: T
authors.initials: T
authors.lastname: Shioda
authors.forename: M
authors.initials: M
authors.lastname: Tatsumi
authors.forename: X
authors.initials: X
authors.lastname: Xin
authors.forename: D
authors.initials: D
authors.lastname: Yu
authors.forename: S
authors.initials: S
authors.lastname: Ohgimoto
authors.forename: A
authors.initials: A
authors.lastname: Kato
authors.forename: Y
authors.initials: Y
authors.lastname: Sakai
authors.forename: Y
authors.initials: Y
authors.lastname: Ohnishi
authors.forename: Y
authors.initials: Y
authors.lastname: Nagai
mesh_terms: D000595:Amino Acid Sequence; D019943:Amino Acid Substitution; D002461:Cell Line, Transformed; D015726:Giant Cells / Q000821:virology*; D006031:Glycosylation; D015699:HIV Envelope Protein gp120 / Q000235:genetics / Q000378:metabolism*; D015497:HIV-1 / Q000302:isolation & purification / Q000378:metabolism* / Q000502:physiology; D006801:Humans; D008969:Molecular Sequence Data; D016297:Mutagenesis, Site-Directed; D010446:Peptide Fragments / Q000235:genetics / Q000378:metabolism*; D011134:Polysaccharides / Q000502:physiology*; D019713:Receptors, CCR5 / Q000502:physiology*; D019718:Receptors, CXCR4 / Q000502:physiology*; D015728:Receptors, HIV / Q000502:physiology; D014779:Virus Replication
chemical_list: D015699:HIV Envelope Protein gp120; C059762:HIV envelope protein gp120 (305-321); D010446:Peptide Fragments; D011134:Polysaccharides; D019713:Receptors, CCR5; D019718:Receptors, CXCR4; D015728:Receptors, HIV