[An experimental study on the anticancerous effects of CD3-TIL derived from human primary hepatic carcinoma].
In this study, CD3-TIL produced by in vito induction with CD3 and IL-2 (CD3-TIL) was compared with TIL stimulated by only IL-2 (TIL) in their in vitro proliferation, and both in vitro and in vivo anticancerous effects. It was found that CD3 at the concentration of 100 ng/ml was the most suitable stimulus of in vitro TIL proliferation (CD3-TIL) which was significantly greater than that by simple IL-2 stimulation. Also in vitro anticancerous activity of CD3-TIL was greater than TIL. CD3-TIL significantly inhibited tumor growth, causing shrinkage of tumor mass, and prolonging the life span of the nude mouse with tumor burden.
Li
A
A
Eastern Hepatobiliary Surgery Hospital, Second Military Medical University, Shanghai.
Wu
M
M
Sheng
F
F
D000818:Animals; D017252:CD3 Complex / Q000276:immunology*; D003602:Cytotoxicity, Immunologic; D005260:Female; D006801:Humans; D016219:Immunotherapy, Adoptive; D008113:Liver Neoplasms / Q000473:pathology*; D016246:Lymphocytes, Tumor-Infiltrating / Q000276:immunology*; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D009368:Neoplasm Transplantation; D014407:Tumor Cells, Cultured
D017252:CD3 Complex