Technical note: Aberrant detection of cell surface Fas ligand with anti-peptide antibodies.
Polyclonal rabbit Abs raised against peptides from the C-terminal region (the extracellular domain) of human Fas ligand were produced for the detection of the molecule in Western blot analysis and immunohistochemistry. These Abs have been used by several groups of investigators to assess cell surface Fas ligand via flow cytometry, but we show that these polyclonal rabbit Abs do not detect cell surface Fas ligand by that technique.
Smith
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Institute of Pathology, Case Western Reserve University, Cleveland, OH 44120, USA.
Sieg
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Kaplan
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D000818:Animals; D000906:Antibodies / Q000276:immunology*; D000918:Antibody Specificity; D015153:Blotting, Western / Q000379:methods; D053222:Fas Ligand Protein; D006801:Humans; D007150:Immunohistochemistry / Q000379:methods; D008562:Membrane Glycoproteins / Q000032:analysis* / Q000276:immunology; D010446:Peptide Fragments / Q000276:immunology*; D011817:Rabbits
D000906:Antibodies; C505661:FASLG protein, human; D053222:Fas Ligand Protein; D008562:Membrane Glycoproteins; D010446:Peptide Fragments