title: Cleaning of endodontic files, Part I: The effect of bioburden on the sterilization of endodontic files.
abstract: Ninety-two new endodontic files were randomly assigned to five groups with varying parameters of contamination, cleaning method, and sterilization (steam or chemical). Files were instrumented in bovine teeth to accumulate debris and a known contaminant, Bacillus stearothermophilus. Positive controls produced growth on both T-soy agar plates and in T-soy broth. Negative controls and experimental files (some with heavy debris) failed to produce growth. The results showed that there was no significant difference between contaminated files that were not cleaned before sterilization and contaminated files that were cleaned before sterilization. Bioburden present on endodontic files does not appear to affect the sterilization process.
authors.forename: M A
authors.initials: MA
authors.lastname: Johnson
authors.forename: P D
authors.initials: PD
authors.lastname: Primack
authors.forename: R J
authors.initials: RJ
authors.lastname: Loushine
authors.forename: D W
authors.initials: DW
authors.lastname: Craft
mesh_terms: D000818:Animals; D002417:Cattle; D016009:Chi-Square Distribution; D003762:Dental Instruments / Q000382:microbiology*; D004866:Equipment Contamination; D001411:Geobacillus stearothermophilus; D018915:Root Canal Preparation / Q000295:instrumentation*; D013242:Sterilization / Q000379:methods*; D014465:Ultrasonics