Collagenase-1 complexes with alpha2-macroglobulin in the acute and chronic wound environments.
The purpose of this study was to examine the appearance and activation of collagenase-1 (MMP-1) in the wound environment. We found that MMP-1 accumulates in the fluid phase of the burn wound environment within 2 d of injury and reaches maximal levels by day 4. Two forms of the enzyme were evident; one that corresponded to proMMP-1 and another that corresponded to a group of high molecular mass (approximately 200 kDa and >200 kDa doublet) MMP-1 containing complexes. ProMMP-1 and MMP-1 containing complexes also occurred in wound fluid from venous stasis ulcers, but neither was detected in mastectomy fluid or in plasma. Levels of the proteinase inhibitor alpha2-macroglobulin in burn fluid and chronic ulcer wound fluid were almost as high as in plasma, and the high molecular mass MMP-1 containing complexes in burn fluid appeared to result from binding between alpha2-macroglobulin and activated MMP-1. These observations provide direct evidence that active MMP-1 in the fluid phase of the wound environment becomes complexed to alpha2-macroglobulin.
Grinnell
F
F
Department of Cell Biology and Neuroscience, University of Texas Southwestern Medical School, Dallas 75235, USA.
Zhu
M
M
Parks
W C
WC
D000208:Acute Disease; D001790:Blood Physiological Phenomena; D001826:Body Fluids / Q000378:metabolism; D002056:Burns / Q000378:metabolism; D002908:Chronic Disease; D017364:Collagenases / Q000737:chemistry / Q000378:metabolism*; D017077:Culture Media, Conditioned / Q000494:pharmacology; D004789:Enzyme Activation / Q000502:physiology; D005347:Fibroblasts / Q000378:metabolism; D006801:Humans; D020781:Matrix Metalloproteinase 1; D008970:Molecular Weight; D014947:Wounds and Injuries / Q000378:metabolism*; D000511:alpha-Macroglobulins / Q000378:metabolism*
D017077:Culture Media, Conditioned; D000511:alpha-Macroglobulins; D017364:Collagenases; D020781:Matrix Metalloproteinase 1