Collagenolytic activity of keratocytes cultured in a collagen matrix.
To study the mechanism of collagen degradation by keratocytes, we developed the new in vitro model in which keratocytes were cultured three-dimensionally in a collagen matrix. Subcultured rabbit keratocytes were embedded in a type I collagen matrix and cultured in serum-free medium. Collagenolytic activity of the cells was determined by measuring the amount of hydroxyproline released into the medium from degraded collagen. Activities of collagenase in the medium were also measured, using collagen labeled with fluorescein isothiocyanate as a substrate. The presence of plasminogen was required for collagen degradation by keratocytes. In the presence of plasminogen, the amount of collagen degradation depended on both the cultivation period and the number of cells. The addition of interleukin-1 (IL-1) stimulated the collagen degradation in a dose-dependent manner. This stimulatory effect of IL-1 was completely inhibited by the addition of IL-1 receptor antagonist (IL-1ra). Collagenase activity in the medium was stimulated by the addition of IL-1, and IL-1ra antagonized this stimulatory effect. These findings indicate that our present model may be useful for investigating the mechanism of collagen degradation by keratocytes.
Mishima
H
H
Department of Ophthalmology, Kinki University School of Medicine, Osaka, Japan.
Okamoto
J
J
Nakamura
M
M
Wada
Y
Y
Otori
T
T
D000818:Animals; D002478:Cells, Cultured; D003094:Collagen / Q000187:drug effects / Q000378:metabolism*; D017364:Collagenases / Q000187:drug effects / Q000378:metabolism; D003315:Cornea / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D003470:Culture Media; D006133:Growth Substances / Q000494:pharmacology; D053590:Interleukin 1 Receptor Antagonist Protein; D007378:Interleukins / Q000494:pharmacology; D008297:Male; D010958:Plasminogen / Q000494:pharmacology; D011817:Rabbits; D017472:Receptors, Interleukin-1 / Q000037:antagonists & inhibitors; D012795:Sialoglycoproteins / Q000494:pharmacology
D003470:Culture Media; D006133:Growth Substances; D053590:Interleukin 1 Receptor Antagonist Protein; D007378:Interleukins; D017472:Receptors, Interleukin-1; D012795:Sialoglycoproteins; D010958:Plasminogen; D003094:Collagen; D017364:Collagenases