Identification of Eucalyptus citriodora clones micropropagated in tissue culture.
The extent of genetic identity observed in the young individuals which were micropropagated from a single Eucalyptus individual was analyzed by using DNA-fingerprinting. Among 40,000 tissue-cultured-seedings of E.citriodora, 200 plants were randomly chosen so that each total DNA might be extracted from their leaves. Using these DNAs as template, PCR was performed with some primers we found in advance that leads polymorphism for DNA of E. citriodora. In this study, all over the 200 cases, the band pattern formed cDNA fragment on a gel after electrophoresis was the identical one mutually.
Kojima
E
E
Biochemistry Section, Iwakuni Research Laboratory of Technology, Nippon Paper Industries Co. Ltd, Yamaguchi, Japan.
Izumi
M
M
Tanabe
T
T
Matsuda
M
M
Shimizu
T
T
Murakami
A
A
Murakami
K
K
D001483:Base Sequence; D018929:Cell Culture Techniques / Q000379:methods; D002999:Clone Cells; D016172:DNA Fingerprinting; D017931:DNA Primers; D018744:DNA, Plant / Q000737:chemistry* / Q000235:genetics / Q000302:isolation & purification; D004587:Electrophoresis, Agar Gel; D018515:Plant Leaves; D016133:Polymerase Chain Reaction / Q000379:methods; D019105:Random Amplified Polymorphic DNA Technique; D013698:Templates, Genetic; D014197:Trees / Q000166:cytology / Q000235:genetics*
D017931:DNA Primers; D018744:DNA, Plant