Preparation and characterization of antibacterial alginate film containing DNA as a carrier of silver ion.
Silver ion was effectively bound to alginate film by the aid of DNA. Amounts of Ag(I) bound to the films (18 mm x 18 mm) were 4.2 micrograms and 24.7 micrograms in the absence and presence of DNA, respectively. The Ag(I)-induced films showed antibacterial activity for both of Escherichiacoli (E. coli) and Staphylococcus aureus (S. aureus), and the activity of DNA-alginate film was obviously larger than alginate film. These results indicate that Ag(I) induced in the DNA-alginate film keeps the potent antibacterial activity, and so DNA is very useful as a carrier of silver ion in the alginate film.
Kitamura
H
H
Division of Bioscience, Graduate School of Environmental Earth Science, Hokkaido University, Sapporo, Japan.
Kondo
Y
Y
Sakairi
N
N
Nishi
N
N
D000464:Alginates / Q000737:chemistry / Q000494:pharmacology*; D000818:Animals; D000900:Anti-Bacterial Agents / Q000737:chemistry / Q000494:pharmacology*; D004247:DNA / Q000737:chemistry* / Q000494:pharmacology*; D004926:Escherichia coli / Q000187:drug effects; D020723:Glucuronic Acid; D006603:Hexuronic Acids; D008826:Microbial Sensitivity Tests; D012474:Salmon; D012834:Silver / Q000737:chemistry / Q000494:pharmacology*; D013211:Staphylococcus aureus / Q000187:drug effects
D000464:Alginates; D000900:Anti-Bacterial Agents; D006603:Hexuronic Acids; D012834:Silver; D020723:Glucuronic Acid; D004247:DNA