Regulation of intracellular metabolism by biodegradable polyrotaxanes.
Cellular response to our designed biodegradable polyrotaxanes was investigated in terms of changes in cytoplasmic calcium levels in platelets. The polyrotaxanes regulated thrombin-induced calcium increase in platelets although constituent molecules of the polyrotaxanes showed fewer effects on the intracellular metabolism. Further, an increase in membrane fluidity of red blood cell ghosts was significantly observed by the addition of the polyrotaxanes. Static light scattering study revealed that the polyrotaxanes formed a supramolecular association state in relation to the molecular weight of PEG: a loosely packed association with a specific molecular shape. From these characteristics, it is suggested that supramolecular level interactions between the polyrotaxanes and cell membranes regulate the intracellular metabolism. It is concluded that these biodegradable polyrotaxanes can be feasible as temporarily-controlled bioactivator.
Ooya
T
T
School of Materials Science, Japan Advanced Institute of Science and Technology, Tatsunokuchi, Ishikawa.
Kumeno
T
T
Yui
N
N
D000818:Animals; D001672:Biocompatible Materials / Q000494:pharmacology*; D001673:Biodegradation, Environmental; D001792:Blood Platelets / Q000187:drug effects / Q000378:metabolism; D002118:Calcium / Q000097:blood; D003505:Cyclodextrins / Q000138:chemical synthesis / Q000494:pharmacology*; D003593:Cytoplasm / Q000187:drug effects / Q000378:metabolism; D004910:Erythrocyte Membrane / Q000187:drug effects; D007424:Intracellular Fluid / Q000187:drug effects / Q000378:metabolism*; D008297:Male; D008560:Membrane Fluidity / Q000187:drug effects; D010649:Phenylalanine / Q000494:pharmacology*; D011092:Polyethylene Glycols / Q000138:chemical synthesis / Q000494:pharmacology*; D011817:Rabbits; D047391:alpha-Cyclodextrins
D001672:Biocompatible Materials; D003505:Cyclodextrins; D047391:alpha-Cyclodextrins; D011092:Polyethylene Glycols; D010649:Phenylalanine; D002118:Calcium; C032613:alpha-cyclodextrin