title: Structural studies on a sulfated polysaccharide from an Arthrobacter sp. by NMR spectroscopy and methylation analysis.
abstract: Structural characterization of a sulfated polysaccharide peptidoglycan complex (SP-PG) from an Arthrobacter sp. was performed by NMR spectroscopy and methylation analysis. In order to simplify the analyses, the desulfated SP-PG was used. NMR spectroscopy revealed the presence of a trisaccharide repeating unit and a disaccharide repeating unit. The trisaccharide unit was composed of two galactofuranosides and one glucopyranoside, and the disaccharide unit was of two galactopyranosides, as shown below. The methylation analysis showed that the polysaccharide consists mainly of a 4-linked galactopyranoside, a 6-linked galactopyranoside, a 6-linked galactofuranoside, a 2,6-linked galactofuranoside, a terminal galactopyranoside and a terminal glucopyranoside. These findings confirmed the structure indicated by the NMR spectroscopy. The repeating units determined in this study are novel.
authors.affiliation: Basic Technology Research Laboratory, Daiichi Pharmaceutical, Tokyo, Japan.
authors.forename: K
authors.initials: K
authors.lastname: Yamazaki
authors.forename: K
authors.initials: K
authors.lastname: Inukai
authors.forename: M
authors.initials: M
authors.lastname: Suzuki
authors.forename: H
authors.initials: H
authors.lastname: Kuga
authors.forename: H
authors.initials: H
authors.lastname: Korenaga
mesh_terms: D001173:Arthrobacter / Q000737:chemistry*; D002240:Carbohydrate Sequence; D002247:Carbon Isotopes; D002473:Cell Wall / Q000737:chemistry*; D003903:Deuterium; D008745:Methylation; D008969:Molecular Sequence Data; D019906:Nuclear Magnetic Resonance, Biomolecular; D011135:Polysaccharides, Bacterial / Q000737:chemistry*; D013463:Sulfuric Acid Esters / Q000737:chemistry
chemical_list: D002247:Carbon Isotopes; D011135:Polysaccharides, Bacterial; D013463:Sulfuric Acid Esters; D003903:Deuterium