Study on site specific cleavage of RNA.
The precursor of an RNA molecule from bacteriophage T4 infected Escherichia coli cell (p2Sp1 RNA) has the ability to cleave itself. It has been found that the site specific RNA cleavage reaction occurred at the pyridine-adenosine sequence in the presence of a monovalent cation and a non-ionic detergent. In order to investigate the mechanism of this cleavage reaction, we designed a RNA oligonucleotide (UUUAUU) and this RNA was cleavage activity at the U-A sequence.
Shirakura
H
H
Department of Industrial Chemistry, Chiba Institute of Technology, Japan.
Hosono
K
K
Kawai
G
G
Takai
K
K
Ohtsuki
T
T
Watanabe
K
K
Sakamoto
K
K
Yokoyama
S
S
Takaku
H
H
D000643:Ammonium Chloride; D017122:Bacteriophage T4 / Q000235:genetics; D001483:Base Sequence; D002414:Cations, Monovalent; D003902:Detergents; D004926:Escherichia coli / Q000821:virology; D006868:Hydrolysis; D008958:Models, Molecular; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation; D009841:Oligonucleotides / Q000737:chemistry; D012313:RNA / Q000737:chemistry*; D012322:RNA Precursors / Q000737:chemistry*
D002414:Cations, Monovalent; D003902:Detergents; D009841:Oligonucleotides; D012322:RNA Precursors; D000643:Ammonium Chloride; D012313:RNA