Increased frequency of micronucleated kidney cells in rats exposed to halogenated anaesthetics.
Six halogenated anaesthetics were tested for their ability to induce micronuclei formation in the rat kidney. A statistically significant increase in the frequency of micronucleated cells was detected in rats given a single p.o. dose of 4 mmol/kg of halothane (3.48 x baseline), chloroform (3.32 x baseline), trichloroethylene (3.24 x baseline), sevoflurane (2.98 x baseline), and isoflurane (2.95 x baseline). In contrast, the response was substantially negative in rats given the same dose of enflurane. As compared to controls, rats treated with halothane and trichloroethylene displayed a reduction in the frequency of binucleated cells presumably due to a toxicity-induced inhibition of cellular proliferation. These findings suggest a potential genotoxic activity of halogenated anaesthetics for the rat kidney.
Robbiano
L
L
Department of Internal Medicine, University of Genova, Italy.
Mereto
E
E
Migliazzi Morando
A
A
Pastore
P
P
Brambilla
G
G
D018685:Anesthetics, Inhalation / Q000633:toxicity*; D000818:Animals; D002725:Chloroform / Q000633:toxicity; D006221:Halothane / Q000633:toxicity; D007530:Isoflurane / Q000633:toxicity; D007668:Kidney / Q000187:drug effects* / Q000648:ultrastructure; D008297:Male; D008738:Methyl Ethers / Q000633:toxicity; D048629:Micronuclei, Chromosome-Defective; D009153:Mutagens / Q000633:toxicity*; D051381:Rats; D017207:Rats, Sprague-Dawley; D000077149:Sevoflurane; D014241:Trichloroethylene / Q000633:toxicity
D018685:Anesthetics, Inhalation; D008738:Methyl Ethers; D009153:Mutagens; D014241:Trichloroethylene; D000077149:Sevoflurane; D002725:Chloroform; D007530:Isoflurane; D006221:Halothane