Neonatal tolerance to alloantigens is induced by enriched antigen-presenting cells.
Spleen and bone marrow cells from normal or Rag-1-deficient donors are equally competent in their ability to induce neonatal transplantation tolerance in semi-allogeneic hosts, and the latter are also capable of tolerizing fully allogeneic recipients. Both types of donor cells resulted in comparable levels of haemopoietic chimerism in tolerant animals. Lymphoid hyperactivity, however, was absent in animals tolerized with Rag-1-deficient cells. The authors conclude that neonatal tolerance induced with haemopoietic cells requires no donor lymphocytes, and is thus not the result of deficient antigen presentation. Furthermore, the state of tolerance can be dissociated from the lymphoid hyperactivity that requires donor lymphocytes and is regularly scored in conventionally tolerized animals.
Modigliani
Y
Y
Unité d'Immunobiologie, CNRS URA 1961, Institut Pasteur, Paris, France.
Burlen-Defranoux
O
O
Bandeira
A
A
Coutinho
A
A
D000818:Animals; D000831:Animals, Newborn / Q000276:immunology*; D000938:Antigen-Presenting Cells / Q000276:immunology*; D001854:Bone Marrow Cells / Q000378:metabolism; D017690:Cell Transplantation; D016207:Cytokines / Q000235:genetics / Q000378:metabolism; D004268:DNA-Binding Proteins / Q000276:immunology; D006183:H-2 Antigens / Q000276:immunology; D018398:Homeodomain Proteins; D007108:Immune Tolerance; D007519:Isoantigens / Q000276:immunology*; D008213:Lymphocyte Activation / Q000276:immunology; D008214:Lymphocytes / Q000276:immunology; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D008808:Mice, Inbred CBA; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000378:metabolism; D013154:Spleen / Q000166:cytology / Q000378:metabolism
D016207:Cytokines; D004268:DNA-Binding Proteins; D006183:H-2 Antigens; D018398:Homeodomain Proteins; D007519:Isoantigens; D012333:RNA, Messenger; C064658:RAG-1 protein