Effect of 3-O-methylglucose on insulin secretion in the perfused dog and rat pancreas.
We recently reported that the nonmetabolizable glucose analogue, 3-O-methylglucose, stimulates somatostatin secretion in the perfused dog pancreas. In this study, we report that 3-O-methylglucose also stimulates insulin secretion in the dog pancreas. The effect was present at 5.5 mM glucose (p < 0.001) but not at O or 2.7 mM glucose. The inhibitor of glucose metabolism, mannoheptulose, blocked the insulinotropic action of 3-O-methylglucose. In contrast, 3-O-methylglucose had no effect on insulin secretion in the perfused rat pancreas. We conclude that 3-O-methylglucose stimulates insulin secretion in the dog and that the effect requires the presence of stimulatory concentrations of D-glucose.
Lindskog
S
S
Department of Medicine, Lund University, Malmö, Sweden.
Hermansen
K
K
Ahrén
B
B
D019325:3-O-Methylglucose / Q000494:pharmacology*; D000818:Animals; D004285:Dogs; D007328:Insulin / Q000378:metabolism*; D000078790:Insulin Secretion; D008297:Male; D010179:Pancreas / Q000187:drug effects*; D010477:Perfusion; D051381:Rats; D017207:Rats, Sprague-Dawley
D007328:Insulin; D019325:3-O-Methylglucose