Virulent Brucella abortus prevents lysosome fusion and is distributed within autophagosome-like compartments.
Virulent and attenuated Brucella abortus strains attach to and penetrate nonprofessional phagocytic HeLa cells. Compared to pathogenic Brucella, the attenuated strain 19 hardly replicates within cells. The majority of the strain 19 bacteria colocalized with the lysosome marker cathepsin D, suggesting that Brucella-containing phagosomes had fused with lysosomes, in which they may have degraded. The virulent bacteria prevented lysosome-phagosome fusion and were found distributed in the perinuclear region within compartments resembling autophagosomes.
Pizarro-Cerdá
J
J
Centre d'Immunologie INSERM-CNRS de Marseille-Luminy, Marseille, France.
Moreno
E
E
Sanguedolce
V
V
Mege
J L
JL
Gorvel
J P
JP
D001422:Bacterial Adhesion; D002003:Brucella abortus / Q000472:pathogenicity*; D002402:Cathepsin D / Q000032:analysis; D006367:HeLa Cells; D006801:Humans; D008247:Lysosomes / Q000382:microbiology* / Q000502:physiology; D010588:Phagosomes / Q000382:microbiology*; D014774:Virulence
D002402:Cathepsin D