title: Huntingtin aggregation monitored by dynamic light scattering.
abstract: An initial stage of fibrillogenesis in solutions of glutathione S-transferase-huntingtin (GST-HD) fusion proteins has been studied by using dynamic light scattering. Two GST-HD systems with poly-L-glutamine (polyGln) extensions of different lengths (20 and 51 residues) have been examined. For both systems, kinetics of z-average translation diffusion coefficients (Dapp) and their angular dependence have been obtained. Our data reveal that aggregation does occur in both GST-HD51 and GST-HD20 solutions, but that it is much more pronounced in the former. Thus, our approach provides a powerful tool for the quantitative assay of GST-HD fibrillogenesis in vitro.
authors.affiliation: Institut für Kristallographie, Freie Universität Berlin, Takustr. 6, D-14195 Berlin, Germany.
authors.forename: Y
authors.initials: Y
authors.lastname: Georgalis
authors.forename: E B
authors.initials: EB
authors.lastname: Starikov
authors.forename: B
authors.initials: B
authors.lastname: Hollenbach
authors.forename: R
authors.initials: R
authors.lastname: Lurz
authors.forename: E
authors.initials: E
authors.lastname: Scherzinger
authors.forename: W
authors.initials: W
authors.lastname: Saenger
authors.forename: H
authors.initials: H
authors.lastname: Lehrach
authors.forename: E E
authors.initials: EE
authors.lastname: Wanker
mesh_terms: D005982:Glutathione Transferase / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D006801:Humans; D000071058:Huntingtin Protein; D006816:Huntington Disease / Q000378:metabolism*; D008027:Light; D009419:Nerve Tissue Proteins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D009687:Nuclear Proteins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D011487:Protein Conformation; D011993:Recombinant Fusion Proteins / Q000737:chemistry / Q000378:metabolism; D013057:Spectrum Analysis
chemical_list: C086055:HTT protein, human; D000071058:Huntingtin Protein; D009419:Nerve Tissue Proteins; D009687:Nuclear Proteins; D011993:Recombinant Fusion Proteins; D005982:Glutathione Transferase