Long-term administration of "sho-saiko-to" increases cytochrome P-450 mRNA level in mouse liver.
Simplified differential display of mRNA was applied to isolate and identify genes transcriptionally regulated in mouse liver by sho-saiko-to administration. A cDNA fragment up-regulated by sho-saiko-to was isolated and characterized. cDNA sequencing and subsequent database analysis revealed that the fragment showed significant sequence similarity with mouse testosterone 16-alpha-hydroxylase (cytochrome P-450[16alpha]) cDNA. The increased level of mRNA expression of cytochrome P-450(16alpha) in association with sho-saiko-to administration suggests the molecular mechanism of the chemopreventive effect of sho-saiko-to. This result indicates the usefulness of the mRNA differential display technique to investigate the molecular mechanism of Kampo medicine.
Kojima
K
K
Faculty of Pharmaceutical Sciences, Nagoya City University, Nagoya, Japan.
Mizukami
H
H
Tazawa
T
T
Nose
M
M
Inoue
M
M
Ogihara
Y
Y
D000818:Animals; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000494:pharmacology*; D001483:Base Sequence; D003001:Cloning, Molecular; D003577:Cytochrome P-450 Enzyme System / Q000096:biosynthesis*; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D005260:Female; D066298:In Vitro Techniques; D008099:Liver / Q000187:drug effects / Q000201:enzymology*; D051379:Mice; D008813:Mice, Inbred ICR; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000096:biosynthesis*; D039181:Steroid 16-alpha-Hydroxylase; D013268:Stimulation, Chemical
D000894:Anti-Inflammatory Agents, Non-Steroidal; D004365:Drugs, Chinese Herbal; D012333:RNA, Messenger; C018142:saiko-keishi-to; D003577:Cytochrome P-450 Enzyme System; D039181:Steroid 16-alpha-Hydroxylase