title: [Diagnosis of mycotic infections in oncology patients using the polymerase chain reaction].
abstract: Diagnosis of mycotic infections is despite the immense effort devoted to this problem still very inaccurate. A new and promising method is the polymerase chain reaction, PCR, which theoretically can detect a single cell. In their original study the authors decided to develop a new method for the detection of fungi by PCR and to compare this examination with post-mortem findings. Thus it was possible to determine sufficiently reliably the sensitivity and specificity of the method. For the detection of fungi the authors selected the sequence coding for a small subunit of ribosomal RNA (18S rDNA). The method is able to detect the amount of DNA from some 10-100 cells. The sensitivity was 90% and the specificity 92%. The method is so far too laborious for common practice, its simplification would be however very useful.
authors.affiliation: II. interní klinika FN, Brno-Bohunice.
authors.forename: J
authors.initials: J
authors.lastname: Vorlícek
authors.forename: J
authors.initials: J
authors.lastname: Mayer
authors.forename: A
authors.initials: A
authors.lastname: Kovarík
authors.forename: J
authors.initials: J
authors.lastname: Cíhalová
authors.forename: V
authors.initials: V
authors.lastname: Kubálek
authors.forename: D
authors.initials: D
authors.lastname: Dvoráková
mesh_terms: D000328:Adult; D000368:Aged; D004271:DNA, Fungal / Q000032:analysis; D006801:Humans; D008875:Middle Aged; D009181:Mycoses / Q000150:complications / Q000175:diagnosis*; D009369:Neoplasms / Q000150:complications*; D009894:Opportunistic Infections / Q000150:complications / Q000175:diagnosis*; D016133:Polymerase Chain Reaction
chemical_list: D004271:DNA, Fungal
vernacular_title: Diagnostika mykotických infekcí u onkologicky nemocných pomocí polymerázové retĕzové reakce.