Novel thermally reversible hydrogel as detachable cell culture substrate.
A novel UV crosslinkable co-polymer of 4-(N-cinnamoylcarbamide)methylstyrene (CCMS) and N-isopropylacrylamide (IPAAm) was partially entrapped in traditional tissue-culture-treated polystyrene and crosslinked by UV light irradiation. Dishes modified by this method showed a change in contact angle with respect to temperature as compared to tissue culture polystyrene controls. Surface chemical analysis indicated that the crosslinked hydrogel does not detach from the surface after successive rinsing in ethanol and water, keeping the cells or cell construct free of unwanted soluble polymer after detachment. Cultures of both bovine endothelium and human retinal pigmented epithelium were confirmed to be able to attach and grow on the polymer-modified surfaces morphologically identical to that on control tissue culture polystyrene surfaces. Corresponding to a change in temperature, these cultures would detach and could be transplanted to another culture surface without functional and structural changes. These results show that the new, photo-crosslinkable hydrogel system can utilize the hydrophobic/hydrophilic change of the surface for cell culture detachment while being permanently applicable to any tissue culture geometry.
von Recum
H A
HA
Department of Bioengineering, University of Utah, Salt Lake City 84112, USA.
Kim
S W
SW
Kikuchi
A
A
Okuhara
M
M
Sakurai
Y
Y
Okano
T
T
D000180:Acrylic Resins / Q000737:chemistry; D000328:Adult; D000818:Animals; D002417:Cattle; D002448:Cell Adhesion / Q000187:drug effects; D018929:Cell Culture Techniques / Q000379:methods*; D003470:Culture Media; D004577:Electron Probe Microanalysis; D005782:Gels; D006801:Humans; D008422:Materials Testing; D011108:Polymers / Q000138:chemical synthesis / Q000494:pharmacology; D017550:Spectroscopy, Fourier Transform Infrared; D013343:Styrenes; D013499:Surface Properties; D013696:Temperature
D000180:Acrylic Resins; D003470:Culture Media; D005782:Gels; D011108:Polymers; D013343:Styrenes; C027142:vinyltoluene; C052970:poly-N-isopropylacrylamide