Synthesis and properties of oligonucleotide derivatives containing a hydrophobic fluorescent dye at the 2'-position.
The oligonucleotides containing 2'-(6-dimethylamino-2-naphthamide)uridine [U(DAN)] at the different position were synthesized by the fully automated solid phase phosphoramidite methods. The UV melting profiles for the U(DAN)-modified oligonucleotides with complementary DNA exhibited sigmoidal curves whose shapes were similar to those for the unmodified duplexes.
Mitsui
T
T
Department of Applied Chemistry, Himeji Institute of Technology, Japan.
Hayashi
H
H
Yamana
K
K
Nakano
H
H
D001331:Automation; D001482:Base Composition; D004247:DNA / Q000737:chemistry*; D005456:Fluorescent Dyes; D007202:Indicators and Reagents; D009691:Nucleic Acid Denaturation; D009838:Oligodeoxyribonucleotides / Q000138:chemical synthesis* / Q000737:chemistry; D013816:Thermodynamics
D005456:Fluorescent Dyes; D007202:Indicators and Reagents; D009838:Oligodeoxyribonucleotides; D004247:DNA