title: Solution structure of a syndecan-4 cytoplasmic domain and its interaction with phosphatidylinositol 4,5-bisphosphate.
abstract: Syndecan-4, a transmembrane heparan sulfate proteoglycan, is a coreceptor with integrins in cell adhesion. It has been suggested to form a ternary signaling complex with protein kinase Calpha and phosphatidylinositol 4,5-bisphosphate (PIP2). Syndecans each have a unique, central, and variable (V) region in their cytoplasmic domains, and that of syndecan-4 is critical to its interaction with protein kinase C and PIP2. Two oligopeptides corresponding to the variable region (4V) and whole domain (4L) of syndecan-4 cytoplasmic domain were synthesized for nuclear magnetic resonance (NMR) studies. Data from NMR and circular dichroism indicate that the cytoplasmic domain undergoes a conformational transition and forms a symmetric dimer in the presence of phospholipid activator PIP2. The solution conformations of both free and PIP2-complexed 4V have been determined by two-dimensional NMR spectroscopy and dynamical simulated annealing calculations. The 4V peptide in the presence of PIP2 formed a compact dimer with two twisted strands packed parallel to each other and the exposed surface of the dimer consisted of highly charged and polar residues. The overall three-dimensional structure in solution exhibits a twisted clamp shape having a cavity in the center of dimeric interface. In addition, it has been observed that the syndecan-4V strongly interacts not only with fatty acyl groups but also the anionic head group of PIP2. These findings reveal that PIP2 promotes oligomerization of syndecan-4 cytoplasmic domain for transmembrane signaling and cell-matrix adhesion.
authors.affiliation: Department of Biochemistry, College of Science, Yonsei University, Seoul 120-740, Korea.
authors.forename: D
authors.initials: D
authors.lastname: Lee
authors.forename: E S
authors.initials: ES
authors.lastname: Oh
authors.forename: A
authors.initials: A
authors.lastname: Woods
authors.forename: J R
authors.initials: JR
authors.lastname: Couchman
authors.forename: W
authors.initials: W
authors.lastname: Lee
mesh_terms: D000595:Amino Acid Sequence; D002942:Circular Dichroism; D003593:Cytoplasm / Q000737:chemistry*; D019281:Dimerization; D009682:Magnetic Resonance Spectroscopy; D008562:Membrane Glycoproteins / Q000737:chemistry*; D008969:Molecular Sequence Data; D019269:Phosphatidylinositol 4,5-Diphosphate / Q000737:chemistry*; D011487:Protein Conformation; D011509:Proteoglycans / Q000737:chemistry*; D012996:Solutions; D053671:Syndecan-4
chemical_list: D008562:Membrane Glycoproteins; D019269:Phosphatidylinositol 4,5-Diphosphate; D011509:Proteoglycans; D012996:Solutions; D053671:Syndecan-4