Adrenalectomy-induced apoptosis and glial responsiveness during ageing.
Cell death by apoptosis occurs in mature neurones of adult rat dentate gyrus following adrenalectomy. In these studies, apoptosis in the dentate gyrus was induced 3 days after adrenalectomy in male Fischer 344 rats ranging in age from 2-3 to 24-26 months. Glial fibrillary acidic protein and transforming growth factor-beta 1 mRNAs were increased in dentate gyrus of all age groups after adrenalectomy, but there was no effect of age on these responses. Furthermore, corticosterone treatment in the drinking water of adrenalectomized rats prevented both cell death and increases in glial mRNAs in all age groups that were tested (up to 16-18 months). Therefore, adrenalectomy-induced apoptosis can be used to study mechanisms of cell death and survival in aged brain.
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Department of Physiology, Monash University, Clayton, Australia.
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D000311:Adrenal Glands / Q000502:physiology*; D000315:Adrenalectomy; D000375:Aging / Q000502:physiology*; D000818:Animals; D053938:DNA Fragmentation; D018891:Dentate Gyrus / Q000166:cytology / Q000502:physiology*; D005904:Glial Fibrillary Acidic Protein / Q000235:genetics; D017403:In Situ Hybridization; D008297:Male; D009457:Neuroglia / Q000502:physiology*; D015347:RNA Probes; D012333:RNA, Messenger / Q000096:biosynthesis; D051381:Rats; D011916:Rats, Inbred F344; D016212:Transforming Growth Factor beta / Q000235:genetics
D005904:Glial Fibrillary Acidic Protein; D015347:RNA Probes; D012333:RNA, Messenger; D016212:Transforming Growth Factor beta