Aquaporin-1: an osmoinducible water channel in cultured mIMCD-3 cells.
The expressions of aquaporin-1 (AQP-1) in cultured mIMCD-3 cells were studied. There was no detectable AQP-1 in cells grown in serum-containing growth medium (SM, 297 +/- 2 mOsm/kg. H2O). When SM was supplemented with NaCl (406 +/- 2 mOsm/kg. H2O), cellular AQP-1 was induced. A further increase in medium osmolarity with NaCl (493 +/- 3 mOsm/kg. H2O) had conferred cells an 2.5 to 3-fold increase of AQP-1 expression over those grown in the 406 +/- 2 mOsm/kg. H2O medium. Moreover, AQP-1 was found to be translocated from cytosol to membrane. In addition, exposing the mIMCD-3 cells to vasopressin (AVP, 10(-8) M) and/or NaCl-supplemented serum-free media (496 +/- 3 mOsm/kg. H2O) for 6h did not render them to produce AQP-1. However, AQP-1 was induced after 24h of incubation, with an 1.5-fold additive effect by AVP. Our RT-PCR data had confirmed the NaCl inducibility and AVP synergism in AQP-1 expression at both mRNA and protein levels. This suggests a new role for cellular AQP-1 and AVP in overcoming osmotic stress in an in vitro system.
Jenq
W
W
Department of Internal Medicine, James A. Haley V. A. Medical Center, Tampa, Florida 33612, USA. wjenq@com1.med.usf.edu
Mathieson
I M
IM
Ihara
W
W
Ramirez
G
G
D000818:Animals; D051398:Aquaporin 1; D020346:Aquaporins; D001127:Arginine Vasopressin / Q000494:pharmacology; D015153:Blotting, Western; D002462:Cell Membrane / Q000378:metabolism; D002478:Cells, Cultured; D003470:Culture Media; D003593:Cytoplasm / Q000378:metabolism; D007473:Ion Channels / Q000096:biosynthesis* / Q000235:genetics; D051379:Mice; D009994:Osmolar Concentration; D012965:Sodium Chloride / Q000494:pharmacology
C496021:Aqp1 protein, mouse; D020346:Aquaporins; D003470:Culture Media; D007473:Ion Channels; D001127:Arginine Vasopressin; D051398:Aquaporin 1; D012965:Sodium Chloride