Testing of toxic and DNA-damaging effects of N-cyclohexylthiophthalimide (Duslin P) on hamster V79 cells.
N-cyclohexylthiophthalimide, commercial name Duslin P, at concentrations 0.5-2 micrograms/ml inhibited proliferation of V79 cells and at concentrations > 2 micrograms/ml acted cytotoxically. Inhibition of cumulative DNA synthesis correlated well with the deleterious effects of Duslin P on growth activity and plating efficiency. DNA synthesis was not renewed even 6 h after the treatment of cells. Alkaline elution of DNA of V79 cells treated with Duslin P did not confirm our expectation that this chemical compound has a DNA-damaging effect. Duslin P strongly inhibited protein synthesis at concentrations > 2 micrograms/ml. We suggest that the cytotoxic effects of Duslin P are not accompanied by any genotoxic effects.
Slamenová
D
D
Cancer Research Institute, Slovak Academy of Sciences, Bratislava, Slovak Republic.
Horváthová
E
E
Farkasová
T
T
Ruzeková
L
L
Bacová
G
G
Krcmáriková
J
J
D000818:Animals; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D006224:Cricetinae; D003412:Cricetulus; D004247:DNA / Q000187:drug effects*; D004249:DNA Damage; D019384:Nucleic Acid Synthesis Inhibitors / Q000633:toxicity*; D010797:Phthalimides / Q000633:toxicity*; D011500:Protein Synthesis Inhibitors / Q000633:toxicity*
D019384:Nucleic Acid Synthesis Inhibitors; D010797:Phthalimides; D011500:Protein Synthesis Inhibitors; C052481:N-(cyclohexylthio)phthalimide; D004247:DNA