title: Endogenous tumour necrosis factor-alpha sensitise melanoma cells to glucosaminylmuramyl dipeptide.
abstract: Flow cytometry was used to demonstrate that cultured human melanoma BRO cells expressed membrane-bound tumour necrosis factor-alpha (TNF-alpha) and were able to release TNF-alpha upon treatment with glucosaminylmuramyl dipeptide (GMDP). The released TNF-alpha was shown to prime melanoma cells, previously unable to respond to GMDP by increasing expression of melanoma-associated antigens, making them sensitive to GMDP treatment.
authors.affiliation: Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Moscow.
authors.forename: T I
authors.initials: TI
authors.lastname: Valyakina
authors.forename: R L
authors.initials: RL
authors.lastname: Komaleva
authors.forename: E E
authors.initials: EE
authors.lastname: Petrova
authors.forename: A A
authors.initials: AA
authors.lastname: Malakhov
authors.forename: O G
authors.initials: OG
authors.lastname: Shamborant
authors.forename: T M
authors.initials: TM
authors.lastname: Andronova
authors.forename: V A
authors.initials: VA
authors.lastname: Nesmeyanov
mesh_terms: D000119:Acetylmuramyl-Alanyl-Isoglutamine / Q000031:analogs & derivatives* / Q000494:pharmacology; D000276:Adjuvants, Immunologic / Q000494:pharmacology*; D000818:Animals; D000951:Antigens, Neoplasm / Q000096:biosynthesis / Q000187:drug effects; D002474:Cell-Free System / Q000502:physiology; D017077:Culture Media, Conditioned / Q000494:pharmacology; D005434:Flow Cytometry; D006801:Humans; D008545:Melanoma / Q000276:immunology / Q000378:metabolism*; D051379:Mice; D014407:Tumor Cells, Cultured; D014409:Tumor Necrosis Factor-alpha / Q000502:physiology*
chemical_list: D000276:Adjuvants, Immunologic; D000951:Antigens, Neoplasm; D017077:Culture Media, Conditioned; D014409:Tumor Necrosis Factor-alpha; D000119:Acetylmuramyl-Alanyl-Isoglutamine; C023868:N-acetyl-beta-glucosaminyl-N-acetylmuramyl-alanylisoglutamine