[The inhibition effect of foreign retinoblastoma gene mediated by recombinant adenovirus vector on the growth of smooth muscle cells].
OBJECTIVE
To study the effect of foreign retinoblastoma (Rb) gene mediated by recombinant adenovirus vector on vascular smooth muscle cell (SMC) and the possibility by using Rb gene for gene therapy of atherosclerosis and artery restenosis.


METHODS
Rb gene recombinant adenovirus vector was constructed to transfect into rabbits' aortic SMC in vitro. The efficiency of transfection and expression was detected by biochemical staining, immunocytochemical analysis and polymerase chain reaction technique. The role of Rb gene for SMC proliferation, DNA synthesis and cell cycle were observed by cell counting. 3H-incorporation and cytometer respectively.


RESULTS
Adenovirus vector transfered the Rb gene into SMC effectively. The expression of Rb gene restricted the proliferation of SMC, decreased the DNA synthesis and plaaied a role in the cell cycle.


CONCLUSIONS
Rb gene mediated by adenovirus can be used to treat atherosclerosis.
Xia
Y
Y
Department of Biochemistry, Beijing Institute of Geriatrics.
Jiang
L
L
Li
J
J
D000260:Adenoviruses, Human / Q000235:genetics; D000818:Animals; D001013:Aorta, Thoracic / Q000166:cytology; D001161:Arteriosclerosis / Q000628:therapy; D002455:Cell Division / Q000187:drug effects; D002478:Cells, Cultured; D016161:Genes, Retinoblastoma / Q000502:physiology*; D015316:Genetic Therapy; D005822:Genetic Vectors; D009131:Muscle, Smooth, Vascular / Q000166:cytology*; D011817:Rabbits; D011994:Recombinant Proteins / Q000494:pharmacology*
D011994:Recombinant Proteins