title: Functional role for the c-Abl tyrosine kinase in meiosis I.
abstract: The c-Abl tyrosine kinase is activated by ionizing radiation and certain other DNA-damaging agents. The DNA-dependent protein kinase (DNA-PK) and the ataxia telangiectasia mutated (ATM) gene product, effectors in the DNA damage response, contribute to the induction of c-Abl activity. The present study demonstrates that c-Abl is expressed in mouse and rat testes, and predominantly in pachytene spermatocytes of meiosis I. The results also demonstrate that c-Abl interacts directly with meiotic chromosomes. In concert with a requirement for c-Abl at the pachytene stage, we show that, in contrast to wild-type mice, testes from Abl-/- mice exhibit defects in spermatogenesis. These findings provide the first demonstration that c-Abl plays a functional role in meiosis.
authors.affiliation: Cancer Pharmacology, Dana-Farber Cancer Institute, Harvard Medical School, Boston, Massachusetts 02115, USA.
authors.forename: S
authors.initials: S
authors.lastname: Kharbanda
authors.forename: P
authors.initials: P
authors.lastname: Pandey
authors.forename: P L
authors.initials: PL
authors.lastname: Morris
authors.forename: Y
authors.initials: Y
authors.lastname: Whang
authors.forename: Y
authors.initials: Y
authors.lastname: Xu
authors.forename: S
authors.initials: S
authors.lastname: Sawant
authors.forename: L J
authors.initials: LJ
authors.lastname: Zhu
authors.forename: N
authors.initials: N
authors.lastname: Kumar
authors.forename: Z M
authors.initials: ZM
authors.lastname: Yuan
authors.forename: R
authors.initials: R
authors.lastname: Weichselbaum
authors.forename: C L
authors.initials: CL
authors.lastname: Sawyers
authors.forename: T K
authors.initials: TK
authors.lastname: Pandita
authors.forename: D
authors.initials: D
authors.lastname: Kufe
mesh_terms: D000818:Animals; D002875:Chromosomes / Q000502:physiology; D015151:Immunoblotting; D007150:Immunohistochemistry; D008297:Male; D008540:Meiosis / Q000235:genetics / Q000502:physiology*; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D011494:Protein Kinases / Q000378:metabolism; D011505:Protein-Tyrosine Kinases / Q000096:biosynthesis / Q000235:genetics / Q000502:physiology*; D016315:Proto-Oncogene Proteins c-abl / Q000096:biosynthesis / Q000235:genetics / Q000502:physiology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D013090:Spermatocytes / Q000378:metabolism / Q000502:physiology / Q000648:ultrastructure; D013091:Spermatogenesis / Q000235:genetics / Q000502:physiology; D013194:Staining and Labeling
chemical_list: D011494:Protein Kinases; D011505:Protein-Tyrosine Kinases; D016315:Proto-Oncogene Proteins c-abl