title: Activation of extracellular matrix metalloproteinases in equine laminitis.
abstract: Samples of connective tissue obtained from the hoof of six laminitic and eight non-laminitic adult horses were analysed zymographically to investigate whether connective tissue matrix metalloproteinases are activated or induced during laminitis. The activity or matrix metalloproteinases was substantially greater in the tissues from the laminitic horses than in the tissues from the non-laminitic horses. A comparison of the collagenolytic activity in the laminitic and control tissues showed that collagenolytic activities corresponding to the 92 kDa (P < 0.001), 72 kDa (P < 0.01) and 66 kDa (P < 0.01) bands were induced in the laminitic tissues.
authors.affiliation: Department of Veterinary Medicine and Surgery, University of Missouri-Columbia 65211, USA.
authors.forename: P J
authors.initials: PJ
authors.lastname: Johnson
authors.forename: S C
authors.initials: SC
authors.lastname: Tyagi
authors.forename: L C
authors.initials: LC
authors.lastname: Katwa
authors.forename: V K
authors.initials: VK
authors.lastname: Ganjam
authors.forename: L A
authors.initials: LA
authors.lastname: Moore
authors.forename: J M
authors.initials: JM
authors.lastname: Kreeger
authors.forename: N T
authors.initials: NT
authors.lastname: Messer
mesh_terms: D000818:Animals; D005109:Extracellular Matrix / Q000201:enzymology; D005260:Female; D005534:Foot Diseases / Q000201:enzymology / Q000662:veterinary*; D006724:Hoof and Claw / Q000201:enzymology* / Q000473:pathology; D006734:Horse Diseases / Q000201:enzymology* / Q000473:pathology; D006736:Horses; D008297:Male; D008666:Metalloendopeptidases / Q000378:metabolism*
chemical_list: D008666:Metalloendopeptidases