Effect of indigestible saccharides on B lymphocyte response of intestinal mucosa and cecal fermentation in rats.
The effects of water-soluble and -insoluble indigestible saccharides (IDS) on immune responses of the intestinal tract were studied. Male 4-week-old Sprague Dawley rats were fed for three weeks on diets containing several kinds of IDS at 5%. The results revealed that the proportion of kappa-light chain and IgA-presenting lymphocytes in small intestinal and cecal mucosa differed in increased number depending on the type of IDS. The response of colonic mucosa was not pronounced. The amounts of short-chain fatty acid (SCFA) and lactic acid in the cecal contents of the other test groups except the celfur group tended to be higher than those in the cellulose group, particularly in the lactulose group where many acids showed significant increases. The correlation between the proportion of kappa-light chain and IgA-presenting lymphocytes in the cecal mucosa and lactic acid in the cecal contents was significant, but that between the proportion of both lymphocytes and SCFA was not. Based on the above, we concluded that the oral administration of IDS induces the proliferation of kappa-light chain and IgA-producing B lymphocytes in small intestinal and cecal mucosa, but the degree of response differs depending on the type of IDS. It is thus suggested that IDS are involved in the intestinal immune system of rats.
Kudoh
K
K
Department of Nutrition, Faculty of Agriculture, Tokyo University of Agriculture, Japan.
Shimizu
J
J
Wada
M
M
Takita
T
T
Kanke
Y
Y
Innami
S
S
D000818:Animals; D001402:B-Lymphocytes / Q000276:immunology*; D002432:Cecum / Q000378:metabolism*; D002482:Cellulose / Q000008:administration & dosage; D004040:Dietary Carbohydrates / Q000378:metabolism* / Q000494:pharmacology*; D004063:Digestion; D005285:Fermentation / Q000187:drug effects*; D006170:Gum Arabic / Q000494:pharmacology; D007070:Immunoglobulin A / Q000032:analysis; D007145:Immunoglobulin kappa-Chains / Q000032:analysis; D007413:Intestinal Mucosa / Q000187:drug effects / Q000276:immunology*; D007792:Lactulose / Q000008:administration & dosage; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley
D004040:Dietary Carbohydrates; D007070:Immunoglobulin A; D007145:Immunoglobulin kappa-Chains; D007792:Lactulose; D006170:Gum Arabic; D002482:Cellulose