title: BDNF induction of tryptophan hydroxylase mRNA levels in the rat brain.
abstract: We have previously demonstrated an augmentation of serotonergic activity within various brain areas following infusion of brain-derived neurotrophic factor (BDNF) into the midbrain near the periaqueductal gray and dorsal and median raphe nuclei (PAG/DR). However, the mechanism of this BDNF-induced modulatory effect on serotonergic systems was unclear. The aim of the present work was to study the regulation of tryptophan hydroxylase (TPH) mRNA levels after chronic BDNF administration in vivo. TPH mRNA levels were measured using a quantitative competitive reverse transcription polymerase chain reaction (RT-PCR) assay. A significant increase in the expression of TPH mRNA (13-fold) was found within the PAG/DR as early as 24 hr after onset of BDNF infusion and was sustained throughout the duration of infusion (11 days). This was accompanied by increased serotonin (5-hydroxytryptamine, 5-HT) levels and decreased nociceptive responsiveness assessed by tail-flick latency. BDNF induction of TPH mRNA levels was also observed in a serotonergic cell line derived from raphe neurons, indicating that BDNF can directly regulate TPH mRNA levels. These results suggest that BDNF augments 5-HT synthesis in vivo by directly enhancing steady-state TPH mRNA levels, and subsequently leading to marked behavioral alterations.
authors.affiliation: Regeneron Pharmaceuticals, Tarrytown, New York, USA.
authors.forename: J A
authors.initials: JA
authors.lastname: Siuciak
authors.forename: M S
authors.initials: MS
authors.lastname: Clark
authors.forename: H B
authors.initials: HB
authors.lastname: Rind
authors.forename: S R
authors.initials: SR
authors.lastname: Whittemore
authors.forename: A F
authors.initials: AF
authors.lastname: Russo
mesh_terms: D000818:Animals; D001835:Body Weight / Q000187:drug effects; D001921:Brain / Q000378:metabolism*; D019208:Brain-Derived Neurotrophic Factor / Q000494:pharmacology*; D002478:Cells, Cultured; D007276:Injections, Intraventricular; D010487:Periaqueductal Gray / Q000378:metabolism; D012333:RNA, Messenger / Q000096:biosynthesis*; D011903:Raphe Nuclei / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D011930:Reaction Time / Q000187:drug effects; D012701:Serotonin / Q000378:metabolism; D013623:Tail; D014365:Tryptophan Hydroxylase / Q000096:biosynthesis / Q000235:genetics*
chemical_list: D019208:Brain-Derived Neurotrophic Factor; D012333:RNA, Messenger; D012701:Serotonin; D014365:Tryptophan Hydroxylase