UCH9, a new antitumor antibiotic produced by Streptomyces: I. Producing organism, fermentation, isolation and biological activities.
We developed a microbial prescreen using Bacillus stearothermophilus NUB3620 and bacteriophage TP-68 to detect potential antitumor compounds acting on DNA or topoisomerases. During the course of screening microbial cultures for their antibacteriophage activities, we found that Streptomyces sp. isolated from a soil sample collected in Iwakuni city, Yamaguchi prefecture, Japan, produced a new antitumor antibiotic, UCH9. UCH9 was isolated from culture broth by a combination of EtOAc extraction and column chromatography. UCH9 has a new structure related to the antitumor antibiotic chromomycins. It exhibited antimicrobial activity against Gram-positive organisms. UCH9 also showed cytotoxic activity against HeLa S3 cells with an IC50 value of 13 nM and exhibited antitumor activity in vivo against mouse leukemia P388.
Ogawa
H
H
Tokyo Research Laboratories, Kyowa Hakko Kogyo, Japan.
Yamashita
Y
Y
Katahira
R
R
Chiba
S
S
Iwasaki
T
T
Ashizawa
T
T
Nakano
H
H
D000818:Animals; D000903:Antibiotics, Antineoplastic / Q000302:isolation & purification* / Q000494:pharmacology; D005285:Fermentation; D006367:HeLa Cells; D006801:Humans; D007941:Leukemia P388 / Q000188:drug therapy; D008297:Male; D051379:Mice; D008926:Plicamycin / Q000031:analogs & derivatives* / Q000302:isolation & purification / Q000494:pharmacology; D013302:Streptomyces / Q000145:classification* / Q000378:metabolism
D000903:Antibiotics, Antineoplastic; C110708:UCH 9; D008926:Plicamycin