Nuclear translocation of the catalytic component of DNA-dependent protein kinase upon growth stimulation in normal human T lymphocytes.
DNA-dependent protein kinase (DNA-PK), consisting of the 470-kDa catalytic component (DNA-PKcs) and the DNA-binding regulatory component Ku protein (p70/p80), catalyzes phosphorylation of a variety of DNA replication/transcription/repair factors in the presence of double-stranded DNA. In the resting states of human peripheral blood mononuclear cells, DNA-PK activity and the protein level of DNA-PKcs were very low in the nuclear extracts, but they were high in the whole cell extracts. Depending upon proliferation of the T lymphocytes, DNA-PK activity and the protein level of DNA-PKcs in the nuclear extracts greatly increased. Immunocytochemical analysis suggested translocation of DNA-PKcs from the cytoplasm to the nucleus upon growth stimulation in the T lymphocytes.
Nagasawa
M
M
Department of Pediatrics, School of Medicine, Tokyo Medical and Dental University, Japan.
Watanabe
F
F
Suwa
A
A
Yamamoto
K
K
Tsukada
K
K
Teraoka
H
H
D000595:Amino Acid Sequence; D001692:Biological Transport / Q000502:physiology; D015153:Blotting, Western; D002455:Cell Division / Q000187:drug effects / Q000502:physiology; D002457:Cell Extracts; D002467:Cell Nucleus / Q000378:metabolism*; D002478:Cells, Cultured; D003593:Cytoplasm / Q000378:metabolism; D051747:DNA-Activated Protein Kinase; D004268:DNA-Binding Proteins; D005455:Fluorescent Antibody Technique; D006801:Humans; D015151:Immunoblotting; D008969:Molecular Sequence Data; D009687:Nuclear Proteins; D010835:Phytohemagglutinins / Q000494:pharmacology; D011233:Precipitin Tests; D017346:Protein Serine-Threonine Kinases / Q000032:analysis / Q000302:isolation & purification / Q000378:metabolism*; D013601:T-Lymphocytes / Q000166:cytology* / Q000201:enzymology*
D002457:Cell Extracts; D004268:DNA-Binding Proteins; D009687:Nuclear Proteins; D010835:Phytohemagglutinins; D051747:DNA-Activated Protein Kinase; C497263:PRKDC protein, human; D017346:Protein Serine-Threonine Kinases