title: Recombinant antigens for specific and sensitive serodiagnosis of Latin American tegumentary leishmaniasis.
abstract: The diagnostic potential of recombinant leishmanial antigens for Latin American tegumentary leishmaniasis (LATL) was examined. Two Leishmania (Viannia) peruviana recombinant proteins, T26-U2 and T26-U4, were assessed by their reactivity to detect specific anti-leishmanial antibodies. Seventy-eight individual sera from persons with LATL, 39 from those with other diseases, and 10 negative control sera were tested by Western blotting and enzyme-linked immunosorbent assay. The sensitivity of the test using T26-U2 plus T26-U4 was similar to that obtained with whole parasite extract (92%). However, the specificity obtained using both recombinant antigens (87%) was higher than that of the whole parasite extract (65%). All tests using recombinant proteins (T26-U2, T26-U2 plus T26-U4 or T26-U4) had a higher positive predictive value (89%, 92% and 98%, respectively) than the value obtained using total parasites (81%). Eleven Colombian sera were also tested, and the results indicated that T26-U2 plus T26-U4 could be used successfully in Peru and in other Latin American countries.
authors.affiliation: Instituto de Medicina Tropical Alexander von Humboldt, Universidad Peruana Cayetano Heredia, Lima, Peru.
authors.forename: Y
authors.initials: Y
authors.lastname: Montoya
authors.forename: C
authors.initials: C
authors.lastname: Leon
authors.forename: M
authors.initials: M
authors.lastname: Talledo
authors.forename: O
authors.initials: O
authors.lastname: Nolasco
authors.forename: C
authors.initials: C
authors.lastname: Padilla
authors.forename: U
authors.initials: U
authors.lastname: Muñoz-Najar
authors.forename: D C
authors.initials: DC
authors.lastname: Barker
mesh_terms: D000818:Animals; D000953:Antigens, Protozoan / Q000737:chemistry; D015153:Blotting, Western; D003105:Colombia; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D007891:Leishmania; D007896:Leishmaniasis / Q000175:diagnosis*; D010568:Peru; D011237:Predictive Value of Tests; D011994:Recombinant Proteins / Q000737:chemistry; D012680:Sensitivity and Specificity; D012698:Serologic Tests / Q000379:methods / Q000592:standards
chemical_list: D000953:Antigens, Protozoan; D011994:Recombinant Proteins