[The preliminary study on prevention and treatment of neonatal jaundice].
OBJECTIVE
The purification and identification of heme oxygenase isoforms (HO-1) and HO-2 from human liver were described and Sn-protoporphyrin (SnPP) was used to inhibit HO-1 activity in order to provide a new method for prevention and treatment of neonatal jaundice.


METHODS
Human hepatic microsomal fractions were purified by DEAE-Sephadex A-25 and hydroxylapatite chromatography. The enzyme activities of the two isoforms were detected with and without Snpp.


RESULTS
HO-1 was the predominant form with a ratio of 2.4:1 (HO-1:HO-2). The apparent molecular weight of HO-1 and HO-2 on SDS-PAGE was 30,000 and 36,000 under reducing conditions, respectively. The study also showed that HO-1 was the traditional inducible heme oxygenase and HO-2 was a non-inducible heme oxygenase.


CONCLUSIONS
The efficient inhibition effect of SnPP on HO-1 is suggested.
Xia
Z
Z
Ruijin Hospital Shanghai Second Medical University.
Yu
S C
SC
Li
Y
Y
D004791:Enzyme Inhibitors / Q000494:pharmacology; D006419:Heme Oxygenase (Decyclizing) / Q000037:antagonists & inhibitors / Q000378:metabolism*; D006801:Humans; D007231:Infant, Newborn; D007527:Isoenzymes / Q000378:metabolism*; D007567:Jaundice, Neonatal / Q000201:enzymology / Q000517:prevention & control*; D008099:Liver / Q000201:enzymology*; D008665:Metalloporphyrins / Q000494:pharmacology*; D008861:Microsomes; D011524:Protoporphyrins / Q000494:pharmacology*
D004791:Enzyme Inhibitors; D007527:Isoenzymes; D008665:Metalloporphyrins; D011524:Protoporphyrins; C032628:tin protoporphyrin IX; D006419:Heme Oxygenase (Decyclizing)