[Culture of endothelial cells as an in vitro model for experimental and clinical research].
Culture of endothelial cell is gotten from human umbilical cord by enzymatic digestion. For the growth of cells in culture, medial RPMI 1640 with 20% mixed human serum (NHS) or 20% fetal calf serum (FCS), endothelial cell growth factor (ECGF) and heparin have been used. Plastic, 0.1% gelatin and fibronectin have been used as a fundament. Immune identification of endothelial cells was culture is performed by monoclonal antibodies on vWF:Ag. Homogeneous cell line in culture might be used as in vitro model in both experimental and practice work.
Dzirlo-Todorović
J
J
Klinika za hematologiju, Klinicki centar Sarajevo.
D018929:Cell Culture Techniques / Q000379:methods*; D004730:Endothelium, Vascular / Q000166:cytology*; D006801:Humans; D014470:Umbilical Cord
Kultura endotelne ćelije kao in vitro model za eksperimentalni i prakticni rad.