title: Construction of YAC-based mammalian artificial chromosomes.
abstract: To construct a mammalian artificial chromosome (MAC), telomere repeats and selectable markers were introduced into a 100 kb yeast artificial chromosome (YAC) containing human centromeric DNA. This YAC, which has a regular repeat structure of alpha-satellite DNA and centromere protein B (CENP-B) boxes, efficiently formed MACs that segregated accurately and bound CENP-B, CENP-C, and CENP-E. The MACs appear to be about 1-5 Mb in size and contain YAC multimers. Structural analyses suggest that the MACs have not acquired host sequences and were formed by a de novo mechanism. The accurate segregation of the MACs suggests they have potential as vectors for introducing genes into mammals.
authors.affiliation: Department of Molecular Biology, School of Science, Nagoya University, Japan.
authors.forename: M
authors.initials: M
authors.lastname: Ikeno
authors.forename: B
authors.initials: B
authors.lastname: Grimes
authors.forename: T
authors.initials: T
authors.lastname: Okazaki
authors.forename: M
authors.initials: M
authors.lastname: Nakano
authors.forename: K
authors.initials: K
authors.lastname: Saitoh
authors.forename: H
authors.initials: H
authors.lastname: Hoshino
authors.forename: N I
authors.initials: NI
authors.lastname: McGill
authors.forename: H
authors.initials: H
authors.lastname: Cooke
authors.forename: H
authors.initials: H
authors.lastname: Masumoto
mesh_terms: D000818:Animals; D001324:Autoantigens; D002460:Cell Line; D002461:Cell Line, Transformed; D002503:Centromere / Q000235:genetics; D051936:Centromere Protein B; D002868:Chromosomal Proteins, Non-Histone / Q000737:chemistry / Q000235:genetics; D002875:Chromosomes / Q000235:genetics*; D018244:Chromosomes, Artificial, Yeast / Q000235:genetics*; D002891:Chromosomes, Human, Pair 21 / Q000235:genetics; D003001:Cloning, Molecular; D004276:DNA, Satellite / Q000737:chemistry / Q000235:genetics; D004268:DNA-Binding Proteins; D005822:Genetic Vectors / Q000235:genetics; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D018386:Kinetochores; D016133:Polymerase Chain Reaction; D016615:Telomere / Q000235:genetics; D014162:Transfection
chemical_list: D001324:Autoantigens; C497926:CENPB protein, human; D051936:Centromere Protein B; D002868:Chromosomal Proteins, Non-Histone; D004276:DNA, Satellite; D004268:DNA-Binding Proteins; C060905:centromere protein C; C068688:centromere protein E