title: Novel primers designed for microsatellite loci in Eucalyptus and identification by PCR fingerprints.
abstract: We found a novel PCR-primer which can be used for the identification on "elite-tree-selection". This primer was designed for selective hybridization at the both ends of microsatellite loci, which is well known as one of the most highpervariable region of DNA. After PCR-fingerprinting on five Eucalyptus species (E. globulus, E.citriodora, E.grandis, E. maidenii, E.bicostata), with our primer, DNA-polymorphism was observed all over the cases.
authors.affiliation: Biochemistry Section, Iwakuni Research Laboratory of Technology, Nippon Paper Industries Co. Ltd, Yamaguchi, Japan.
authors.forename: M
authors.initials: M
authors.lastname: Matsuda
authors.forename: E
authors.initials: E
authors.lastname: Kojima
authors.forename: M
authors.initials: M
authors.lastname: Izumi
authors.forename: K
authors.initials: K
authors.lastname: Murakami
mesh_terms: D001483:Base Sequence; D016172:DNA Fingerprinting / Q000379:methods; D017931:DNA Primers; D018744:DNA, Plant / Q000737:chemistry; D018895:Microsatellite Repeats; D016133:Polymerase Chain Reaction / Q000379:methods; D013045:Species Specificity; D014197:Trees / Q000145:classification / Q000235:genetics*
chemical_list: D017931:DNA Primers; D018744:DNA, Plant