[Perfusion of donor tissue improves the preservation of graft in heterotopic tracheal transplantation].
To assess the effect on tracheal graft preservation of perfusion of donor tissue with a Collins solution before extraction and immunosuppression of the recipient. An experimental study was performed in 36 albino rabbits with revascularized heterotopic cervical reconstruction of the trachea with omentum. The animals were distributed in four groups. Groups I (n = 9) and III (n = 9) were transplanted with non perfused donor tissue. Animals in groups II (n = 9) and IV (n = 9) received grafts perfused with Collins solution. Immunosuppression with steroids and cyclosporin was continued for 21 days in groups III and IV. In a mid portion of the trachea viewed under optical microscope, the degree of inflammation or circumferential necrosis was assessed on a scale of 0 to 9 by adding the scores for mucosa, submucosa and cartilage. The mean score for tracheal lesion was lower in group IV, with a likelihood of random difference of less than 5%. Perfusion of peritracheal tissues with Collins solution in the donor, in addition to immunosuppression decreases the extent of tissue damage in the tracheal graft.
Jiménez
M F
MF
Sección de Cirugía Torácica, Hospital Universitario, Universidad de Salamanca.
Gómez-Alonso
A
A
Varela
G
G
Ludeña
M D
MD
García-Criado
J
J
D000701:Analgesics, Opioid / Q000008:administration & dosage; D000818:Animals; D000893:Anti-Inflammatory Agents / Q000008:administration & dosage; D002047:Buprenorphine / Q000008:administration & dosage; D002437:Cefazolin / Q000008:administration & dosage; D002511:Cephalosporins / Q000008:administration & dosage; D003524:Cyclosporins / Q000008:administration & dosage; D003627:Data Interpretation, Statistical; D005260:Female; D006982:Hypertonic Solutions / Q000008:administration & dosage; D007165:Immunosuppression Therapy; D007166:Immunosuppressive Agents / Q000008:administration & dosage; D007249:Inflammation / Q000473:pathology; D008297:Male; D008775:Methylprednisolone / Q000008:administration & dosage; D009336:Necrosis; D010477:Perfusion; D011817:Rabbits; D013997:Time Factors; D014019:Tissue Donors; D014132:Trachea / Q000473:pathology / Q000637:transplantation*
D000701:Analgesics, Opioid; D000893:Anti-Inflammatory Agents; D002511:Cephalosporins; C024102:Collins' solution; D003524:Cyclosporins; D006982:Hypertonic Solutions; D007166:Immunosuppressive Agents; D002047:Buprenorphine; D002437:Cefazolin; D008775:Methylprednisolone
La perfusión tisular del donante mejora la conservación del injerto en el transplante traqueal heterotópico.