Expression of Xenopus laevis translation initiation factor 4E (eIF-4E) by baculovirus-insect cell system.
A gene encoding Xenopus laevis eIF-4E was cloned into a transfer vector, and its gene expression was attempted in cells of E. coli, yeast and insect. Effective expression of the active eIF-4E was achieved in the soluble fraction of the insect cell Sf9, which was infected with the recombinant baculovirus. Overexpression of the eIF-4E protein caused remarkable change in the shape of the cells.
Miyoshi
H
H
Institute for Biomolecular Science, Gakushuin University, Tokyo, Japan.
Ito
K
K
Sakai
N
N
Mizushima
J
J
Okamoto
K
K
Hori
H
H
Nishino
T
T
Wakiyama
M
M
Miura
K
K
D000818:Animals; D016367:Baculoviridae; D002460:Cell Line; D039561:Eukaryotic Initiation Factor-4E; D007700:Kinetics; D010448:Peptide Initiation Factors / Q000096:biosynthesis*; D011994:Recombinant Proteins / Q000096:biosynthesis; D018411:Spodoptera / Q000166:cytology; D014162:Transfection; D014982:Xenopus laevis
D039561:Eukaryotic Initiation Factor-4E; D010448:Peptide Initiation Factors; D011994:Recombinant Proteins