title: pZ402, an improved SV40-based shuttle vector containing a T-antigen mutant unable to interact with wild-type p53.
abstract: Shuttle vectors are useful tools for studying DNA replication and mutagenesis. SV40-based shuttle vectors are popular because of their ease of use and quick results. However, one complication with the use of SV40-based shuttle vectors is the interaction of cellular p53 protein with the T-antigen of SV40. Wild-type, but not mutant p53 has been shown to be involved in DNA replication and DNA repair. To address this concern, we have modified an SV40-based shuttle vector, pZ189, by exchanging the wt T-antigen for a mutant SV40 T-antigen, which is unable to bind with p53. This shuttle vector, pZ402, provides us with a tool to study DNA replication and genomic instability in cells with varying genetic backgrounds without interference from the interaction of T-antigen with p53.
authors.affiliation: Department of Tumor Biology, University of Texas M.D. Anderson Cancer Center, Houston, TX 77030, USA.
authors.forename: E
authors.initials: E
authors.lastname: Kraus
authors.forename: L C
authors.initials: LC
authors.lastname: Strong
authors.forename: M A
authors.initials: MA
authors.lastname: Tainsky
mesh_terms: D019943:Amino Acid Substitution; D000952:Antigens, Polyomavirus Transforming / Q000235:genetics* / Q000378:metabolism; D002460:Cell Line; D003001:Cloning, Molecular; D004261:DNA Replication / Q000235:genetics; D005814:Genes, Viral / Q000235:genetics; D005822:Genetic Vectors / Q000235:genetics*; D006801:Humans; D017354:Point Mutation / Q000235:genetics; D011485:Protein Binding; D014162:Transfection; D016159:Tumor Suppressor Protein p53 / Q000378:metabolism*
chemical_list: D000952:Antigens, Polyomavirus Transforming; D016159:Tumor Suppressor Protein p53