title: Defluorinated sparfloxacin as a new photoproduct identified by liquid chromatography coupled with UV detection and tandem mass spectrometry.
abstract: Photodegradation of sparfloxacin was observed by means of high-pressure liquid chromatography with UV detection and liquid chromatography coupled with UV detection and tandem mass spectrometry (LC-MS/MS). Three products were detected. Comparison with an independently synthesized derivative of sparfloxacin revealed the structure of one product which is believed to be 8-desfluorosparfloxacin. The second product is likely to be formed by the splitting off of a fluorine and a cyclopropyl ring. Thus, photodefluorination of quinolone antibacterial agents is found and proved for the first time by LC-MS/MS.
authors.affiliation: Pharmazeutisches Institut der Universität Bonn, Germany.
authors.forename: M
authors.initials: M
authors.lastname: Engler
authors.forename: G
authors.initials: G
authors.lastname: Rüsing
authors.forename: F
authors.initials: F
authors.lastname: Sörgel
authors.forename: U
authors.initials: U
authors.lastname: Holzgrabe
mesh_terms: D000890:Anti-Infective Agents / Q000737:chemistry* / Q000378:metabolism*; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D024841:Fluoroquinolones; D013058:Mass Spectrometry / Q000379:methods; D010777:Photochemistry; D015363:Quinolones / Q000737:chemistry* / Q000378:metabolism*
chemical_list: D000890:Anti-Infective Agents; D024841:Fluoroquinolones; D015363:Quinolones; C061363:sparfloxacin