[Expression of platelet activation markers--selectin P and glycoprotein CD63 during hemodialysis].
The bleeding tendency is a common feature of chronic uremia. Abnormalities of platelet function play a role in the pathogenesis of this disorder. A direct contact between platelets and an artificial dialysis membrane results in strong activation of thrombolysis. The aim of our study was to investigate platelets activation in vivo during haemodialysis. We used monoclonal antibodies specific against platelet activation markers--selectin P (CD62) and glycoprotein CD63. The expression of those antigens was analyzed by flow cytometry. Blood samples were obtained from 20 long-term haemodialysed patients with end-stage renal disease. After 15 minutes of haemodialysis the expression of CD62 and CD63 was significantly higher (p < 0.001) as compared CD63 glycoprotein. Our results show that haemodialysis has a significant influence on platelet activation and can favour co-existence of the bleeding tendency and the prothrombotic status in long-term hemodialyzed patients.
Walter-Croneck
A
A
Klinika Hematologii Akademii Medycznej w Lublinie.
Belniak-Legieć
E
E
Janicka
L
L
Madro
E
E
Krawczyk
P
P
D000328:Adult; D000368:Aged; D000911:Antibodies, Monoclonal / Q000032:analysis; D015703:Antigens, CD / Q000032:analysis*; D015415:Biomarkers / Q000032:analysis; D005260:Female; D005434:Flow Cytometry; D006470:Hemorrhage / Q000209:etiology*; D006801:Humans; D007676:Kidney Failure, Chronic / Q000097:blood*; D008297:Male; D008875:Middle Aged; D019007:P-Selectin / Q000097:blood*; D015539:Platelet Activation / Q000502:physiology*; D010980:Platelet Membrane Glycoproteins / Q000032:analysis*; D006435:Renal Dialysis / Q000009:adverse effects*; D060149:Tetraspanin 30
D000911:Antibodies, Monoclonal; D015703:Antigens, CD; D015415:Biomarkers; C554586:CD63 protein, human; D019007:P-Selectin; D010980:Platelet Membrane Glycoproteins; D060149:Tetraspanin 30
Ekspresja markerów aktywacji płytek krwi--selektyny p oraz glikoproteiny CD63 w czasie hemodializy.