title: The adsorption of substrate-binding domain of PHB depolymerases to the surface of poly(3-hydroxybutyric acid).
abstract: The binding characteristic of PHB depolymerase has been studied by using glutathione S-transferase (GST) fusion proteins with substrate-binding domain of three bacterial PHB depolymerases, Alcaligenes faecalis, Comamonas acidovorans and Comamonas testosteroni. Analysis using immuno-gold labeling technique and transmission electron microscopy indicated that a novel GST fusion protein derived from A. Faecalis enzyme adsorbed to the surface of poly(3-hydroxybutyric acid) (P(3HB)) single crystals like other fusion proteins. Comparison of inhibiting degree of P(3HB) powder hydrolysis activity of PHB depolymerase by fusion proteins indicated that three fusion proteins bind to P(3HB) powder in the same degree. The measurement of the surface hydrophobicity of proteins suggests that the interaction of the substrate-binding domain with insoluble P(3HB) may include not only a hydrophobic effect but also molecule-specific contacts.
authors.affiliation: Polymer Chemistry Laboratory, Institute of Physical and Chemical Research (RIKEN), Saitama, Japan.
authors.forename: M
authors.initials: M
authors.lastname: Shinomiya
authors.forename: T
authors.initials: T
authors.lastname: Iwata
authors.forename: Y
authors.initials: Y
authors.lastname: Doi
mesh_terms: D000217:Acyltransferases / Q000235:genetics / Q000493:pharmacokinetics*; D000327:Adsorption; D000421:Alcaligenes / Q000201:enzymology; D000595:Amino Acid Sequence; D005982:Glutathione Transferase / Q000235:genetics / Q000493:pharmacokinetics; D016946:Gram-Negative Aerobic Rods and Cocci; D006885:Hydroxybutyrates / Q000493:pharmacokinetics*; D008969:Molecular Sequence Data; D011091:Polyesters / Q000493:pharmacokinetics*; D011993:Recombinant Fusion Proteins / Q000493:pharmacokinetics; D016415:Sequence Alignment; D013499:Surface Properties; D000094122:Polyhydroxybutyrates
chemical_list: D000217:Acyltransferases; D005982:Glutathione Transferase; D006885:Hydroxybutyrates; D011091:Polyesters; D011993:Recombinant Fusion Proteins; C003182:poly-beta-hydroxybutyrate; D000094122:Polyhydroxybutyrates; C060970:poly-beta-hydroxybutyrate polymerase