title: A novel region of the alpha4 integrin subunit with a modulatory role in VLA-4-mediated cell adhesion to fibronectin.
abstract: The integrin VLA-4 (alpha4 beta1) is a receptor for fibronectin and vascular cell-adhesion molecule 1 (VCAM-1). Four functionally different epitopes, designated A, B1, B2 and C, have previously been defined on the alpha4 subunit. Using K562 alpha4 mutant transfectants we found that alpha4 amino acids Tyr151, Gln152, Asp153, Tyr154 and Val155 are important for the structure of the epitope B2. Mutations at alpha4 Gln152 substantially impaired the transfectant adhesion to a CS-1-containing fragment of fibronectin (FN-H89), whereas this adhesion was not affected on the other alpha4 mutant transfectants. None of the alpha4 mutations significantly altered the adhesion of the different alpha4 transfectants to VCAM-1. In addition, we have identified residues Gln152, Asp153 and Tyr154 as part of the alpha4 epitope B2 involved in homotypic cell aggregation. The decrease in adhesion to FN-H89 shown by Gln152 alpha4 mutant transfectants was the result of an inefficient binding of FN-H89 by VLA-4 mutated at this residue. Also, mutant VLA-4 displayed an altered reactivity with HUTS-21, an anti-beta1 monoclonal antibody that reacts with functionally active VLA integrins. Adhesion to FN-H89 was not restored unless stimuli that increase the ligand-binding affinity of VLA heterodimers were added, suggesting that cell adhesion was affected in the initial phases. These results indicate that alpha4 Gln152 modulates cell adhesion to FN-H89 by playing important roles in the maintenance and/or the acquisition of an active state of VLA-4, an integrin that is normally expressed on the cell surface in a range of multiple activation states. The location of the alpha4 Gln152 residue on a loop of the upper surface of the proposed beta-propeller structure suggests a close association with potential ligand-binding sites.
authors.affiliation: Centro de Investigaciones Biológicas, Departamento de Inmunología, Velázquez 144, 28006 Madrid, Spain.
authors.forename: M
authors.initials: M
authors.lastname: Muñoz
authors.forename: J
authors.initials: J
authors.lastname: Serrador
authors.forename: M
authors.initials: M
authors.lastname: Nieto
authors.forename: A
authors.initials: A
authors.lastname: Luque
authors.forename: F
authors.initials: F
authors.lastname: Sánchez-Madrid
authors.forename: J
authors.initials: J
authors.lastname: Teixidó
mesh_terms: D000911:Antibodies, Monoclonal / Q000276:immunology; D015703:Antigens, CD / Q000096:biosynthesis / Q000235:genetics / Q000276:immunology / Q000502:physiology*; D002448:Cell Adhesion / Q000235:genetics; D002449:Cell Aggregation / Q000235:genetics / Q000276:immunology; D000939:Epitopes; D005353:Fibronectins / Q000502:physiology*; D006801:Humans; D039441:Integrin alpha4; D039041:Integrin alpha4beta1; D016023:Integrins / Q000096:biosynthesis / Q000235:genetics / Q000276:immunology / Q000502:physiology*; D036341:Intercellular Signaling Peptides and Proteins; D007694:Killer Cells, Natural; D009154:Mutation; D010455:Peptides / Q000502:physiology; D016201:Receptors, Lymphocyte Homing / Q000096:biosynthesis / Q000235:genetics / Q000276:immunology / Q000502:physiology*; D012995:Solubility; D019010:Vascular Cell Adhesion Molecule-1 / Q000502:physiology
chemical_list: D000911:Antibodies, Monoclonal; D015703:Antigens, CD; D000939:Epitopes; D005353:Fibronectins; D039041:Integrin alpha4beta1; D016023:Integrins; D036341:Intercellular Signaling Peptides and Proteins; D010455:Peptides; D016201:Receptors, Lymphocyte Homing; D019010:Vascular Cell Adhesion Molecule-1; C076687:CS1 peptide; D039441:Integrin alpha4