Characterization of a novel CC chemokine, HCC-4, whose expression is increased by interleukin-10.
We have identified and characterized a human beta (CC) chemokine, designated HCC-4, that is most closely related to HCC-1 and which demonstrates chemotactic activity for monocytes. Northern analysis of multiple tissue blots and of activated monocytes mRNA shows expression of a 500-bp mRNA. A 1,500-bp mRNA was highly expressed in monocytes activated 12 hours in the presence of interleukin-10 (IL-10) but was absent in monocytes activated for only 1 hour regardless of the presence or absence of IL-10. The upregulation of expression in the presence of IL-10 is in contrast to the downregulatory effects of IL-10 on expression of most other chemokines. Recombinant HCC-4 demonstrated chemotactic activity for human monocytes and THP-1 monocyte cells but not for resting lymphocytes or neutrophils. HCC-4 also induced a Ca2+ flux in THP-1 cells that was desensitized by prior exposure to RANTES. Taken together, these data indicate that HCC-4 is a novel chemokine whose expression is uniquely upregulated by IL-10.
Hedrick
J A
JA
DNAX Research Institute, Palo Alto, CA 94304, USA.
Helms
A
A
Vicari
A
A
Zlotnik
A
A
D000595:Amino Acid Sequence; D001483:Base Sequence; D001798:Blood Proteins / Q000737:chemistry / Q000235:genetics; D019742:Chemokines, CC / Q000096:biosynthesis* / Q000737:chemistry / Q000235:genetics; D002886:Chromosomes, Human, Pair 17; D006801:Humans; D016753:Interleukin-10 / Q000494:pharmacology*; D008969:Molecular Sequence Data; D009000:Monocytes / Q000187:drug effects / Q000378:metabolism; D012333:RNA, Messenger / Q000378:metabolism; D011994:Recombinant Proteins / Q000378:metabolism; D016415:Sequence Alignment; D016324:Sequence Tagged Sites; D015854:Up-Regulation
D001798:Blood Proteins; C097720:CCL14 protein, human; C481939:CCL16 protein, human; D019742:Chemokines, CC; D012333:RNA, Messenger; D011994:Recombinant Proteins; D016753:Interleukin-10