Release of nitric oxide from a spin trap, N-tert-butyl-alpha-phenylnitrone, under various oxidative conditions.
Nitric oxide (NO) generation from a spin trap, N-tert-butyl-alpha-phenylnitrone (PBN) under various oxidative conditions was examined. The absorbance of PBN at 295 nm decreased with time of UV-irradiation, showing that PBN was decomposed by UV irradiation. The hydroxyl radical formed from a Fenton reagent also decomposed PBN, but there was little effect by a peroxyl radical and a superoxide. Nitrite, an oxidative product of NO, in PBN solution was determined using a NOx analyzer based on Griess reaction. UV-irradiation and the hydroxyl radical also formed nitrite. Direct detection of NO from the sample on reaction with hydroxyl radical was successful using a GC/MS/SIM on the UV-irradiated sample. NO generated in PBN solutions activated guanylate cyclase. From these results, PBN is viewed as a new kind of medicine which acts as an antioxidant and as an NO donor in vivo.
Saito
K
K
Gerontology Research Center, National Institute on Aging, NIH, Baltimore, MD 21224, USA.
Yoshioka
H
H
Kazama
S
S
Cutler
R G
RG
D000818:Animals; D006152:Cyclic GMP / Q000096:biosynthesis; D003497:Cyclic N-Oxides; D004791:Enzyme Inhibitors / Q000494:pharmacology; D016166:Free Radical Scavengers / Q000737:chemistry*; D005609:Free Radicals; D006162:Guanylate Cyclase / Q000037:antagonists & inhibitors; D017665:Hydroxyl Radical; D066298:In Vitro Techniques; D008099:Liver / Q000187:drug effects / Q000378:metabolism; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D009569:Nitric Oxide / Q000737:chemistry*; D009589:Nitrogen Oxides / Q000737:chemistry*; D010084:Oxidation-Reduction; D013056:Spectrophotometry, Ultraviolet; D019097:Spin Trapping
D003497:Cyclic N-Oxides; D004791:Enzyme Inhibitors; D016166:Free Radical Scavengers; D005609:Free Radicals; D009589:Nitrogen Oxides; D009569:Nitric Oxide; D017665:Hydroxyl Radical; C029217:phenyl-N-tert-butylnitrone; D006162:Guanylate Cyclase; D006152:Cyclic GMP