Effect of nucleotide sequence and metal ions at the active site on a short ribozyme activation.
Leadzyme, CUGGGAGUCC, is a ribozyme that requires Pb2+. We have investigated effects of target sequences and metal ions on a catalytic reaction of the leadyzme. The leadzyme plays as a catalyst for one site cleavage of a mutant substrate, GGAC decrease GAGCCAG, and a wild-type substrate, GGACC decrease GAGCCAG. The addition of 25 microM Nd3+ in the presence of 25 microM Pb2+ led to significant increase in the cleavage yield for the mutant substrate. But this increase of the yield is less than that for the wild-type substrate. The result suggests that the effect of Nd3+ on the leadzyme reaction depends on substrate sequence.
Sugimoto
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N
Department of Chemistry, Faculty of Science, Konan University, Kobe, Japan.
Ohmichi
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T
D001483:Base Sequence; D001665:Binding Sites; D002384:Catalysis; D004789:Enzyme Activation; D007700:Kinetics; D007854:Lead / Q000494:pharmacology*; D009354:Neodymium / Q000494:pharmacology*; D009690:Nucleic Acid Conformation; D009843:Oligoribonucleotides / Q000737:chemistry / Q000378:metabolism; D016337:RNA, Catalytic / Q000737:chemistry* / Q000378:metabolism*; D013379:Substrate Specificity
D009843:Oligoribonucleotides; D016337:RNA, Catalytic; D009354:Neodymium; D007854:Lead