Isolation and identification of CGRP C-terminal fragments in the rat spinal cord.
In this study, we have used a liquid chromatography micropurification system in combination with fast atom bombardment mass spectrometry (FAB-MS) and N-terminal sequencing to characterize 3 calcitonin gene-related peptide (CGRP) immunoreactivities present in the rat spinal cord. Full-length CGRP contributed to approximately 68% of the total immunoreactive material, while approximately 23% consisted of 2 C-terminal fragments, CGRP(18-37) and CGRP(19-37). Synthetic C-terminal fragments of CGRP, e.g. CGRP(19-37), have been shown to antagonize CGRP effects in vitro. We show that such fragments exist in relatively substantial amounts in the rat spinal cord.
Le Grevès
P
P
Department of Pharmaceutical Biosciences, University of Uppsala, Sweden. pierrelegreves@farmbio.uu.se
Andersson
K
K
Silberring
J
J
D000818:Animals; D015740:Calcitonin Gene-Related Peptide / Q000032:analysis*; D002852:Chromatography, Ion Exchange; D008297:Male; D010446:Peptide Fragments / Q000032:analysis*; D011863:Radioimmunoassay; D051381:Rats; D017207:Rats, Sprague-Dawley; D016339:Spectrometry, Mass, Fast Atom Bombardment; D013116:Spinal Cord / Q000737:chemistry*
D010446:Peptide Fragments; C079069:calcitonin gene related peptide (19-37); C079068:calcitonin gene-related peptide (23-37); D015740:Calcitonin Gene-Related Peptide