The gene structure and promoter analysis of mouse lymphocyte signal transduction molecule alpha 4 that is related to the yeast TAP42 involved in a rapamycin-sensitive pathway.
The mouse alpha 4 phosphoprotein encoding a component associated with the B cell antigen receptor (BCR)-mediated signal transduction is suggested to be involved in a unique rapamycin-sensitive pathway. We studied the structure and the molecular mechanism of the expression of alpha 4 gene by isolating two phage clones, named #10 and #23, covering entire exons of the mouse alpha 4 gene. The alpha 4 gene is located within about 25 kb and composed of six exons. To analyze the regulation of alpha 4 gene expression, we determined the nucleotide sequence toward 2 kb upstream of the translation start site of the alpha 4 gene. The 5'-flanking region does not contain a typical TATA box or the initiation consensus sequence, but it contains a CCAAT box, E-boxes, and several DNA binding motifs such as c-Myc, c-Myb, and c-Ets. Transcription of the alpha 4 gene starts at four different sites, determined by primer extension analysis, that were surrounded by Y-rich sequences. We further characterized the functional promoter of the alpha 4 gene at the region between -263 and the transcription start site of alpha 4 gene by luciferase assay system and suggested that the 5' upstream region of alpha 4 gene contains the silencer element of MT repetitive sequence.
Maeda
K
K
Department of Immunology, Kumamoto University School of Medicine, Japan.
Inui
S
S
Sanjo
H
H
Sakaguchi
N
N
D048868:Adaptor Proteins, Signal Transducing; D000818:Animals; D001483:Base Sequence; D001665:Binding Sites; D002460:Cell Line; D002874:Chromosome Mapping; D005656:Fungal Proteins / Q000235:genetics*; D036341:Intercellular Signaling Peptides and Proteins; D008214:Lymphocytes / Q000378:metabolism; D051379:Mice; D018832:Molecular Chaperones; D008969:Molecular Sequence Data; D010442:Peptide Chain Initiation, Translational; D010750:Phosphoproteins / Q000235:genetics*; D011090:Polyenes / Q000494:pharmacology*; D011401:Promoter Regions, Genetic; D029701:Saccharomyces cerevisiae Proteins; D015398:Signal Transduction; D020123:Sirolimus; D014158:Transcription, Genetic
D048868:Adaptor Proteins, Signal Transducing; D005656:Fungal Proteins; C092377:Igbp1 protein, mouse; D036341:Intercellular Signaling Peptides and Proteins; D018832:Molecular Chaperones; D010750:Phosphoproteins; D011090:Polyenes; D029701:Saccharomyces cerevisiae Proteins; C100984:TAP42 protein, S cerevisiae; D020123:Sirolimus