CD3-CD4-CD8-CD56-CD19-CD14- cells and CD3+CD8 dull-positive cells produce IL-4 in AIDS patients.
We used flow cytometry to identify the presence of intracellular cytokines (cytoflow) and analyse the production of IL-4 in peripheral blood from AIDS patients who have practically no CD4+ T cells. We found that IL-4 was produced by CD3-CD4-CD8-CD56-CD19-CD14- cells and CD3+CD8 dull-positive cells in AIDS patients. Moreover, CD3-CD4-CD8-CD56-CD19-CD14- cells had helper activity for immunoglobulin synthesis. These findings indicate that instead of CD4+ T helper cells, C3-CD4-CD8-CD56-CD19-CD14- cells and CD3+CD8 dull-positive cells may be an important source of IL-4 in a variety of immune responses for AIDS patients.
Honda
J
J
First Department of Internal Medicine, Kurume University School of Medicine, Fukuoka, Japan.
Okubo
Y
Y
Tanaka
K
K
Kusaba
M
M
Kumagai
M
M
Saruwatari
N
N
Oizumi
K
K
D000163:Acquired Immunodeficiency Syndrome / Q000276:immunology*; D000328:Adult; D015703:Antigens, CD / Q000276:immunology*; D018941:Antigens, CD19 / Q000276:immunology; D017252:CD3 Complex / Q000276:immunology; D015704:CD4 Antigens / Q000276:immunology; D019002:CD56 Antigen / Q000276:immunology; D016827:CD8 Antigens / Q000276:immunology; D004306:Dose-Response Relationship, Immunologic; D005260:Female; D005434:Flow Cytometry; D006801:Humans; D007074:Immunoglobulin G / Q000378:metabolism; D015847:Interleukin-4 / Q000096:biosynthesis*; D018950:Lipopolysaccharide Receptors / Q000276:immunology; D008297:Male; D008875:Middle Aged; D010641:Phenotype; D006377:T-Lymphocytes, Helper-Inducer / Q000276:immunology / Q000378:metabolism*
D015703:Antigens, CD; D018941:Antigens, CD19; D017252:CD3 Complex; D015704:CD4 Antigens; D019002:CD56 Antigen; D016827:CD8 Antigens; D007074:Immunoglobulin G; D018950:Lipopolysaccharide Receptors; D015847:Interleukin-4