Dystrophin acts as a transplantation rejection antigen in dystrophin-deficient mice: implication for gene therapy.
Duchenne muscular dystrophy is a lethal and common X-linked recessive disease caused by a defect in dystrophin. Normal myoblast transplantation and dystrophin gene transfer have been expected to correct the deficiency in the muscles, but their clinical application has been hampered by the limited preservation of dystrophin-positive myofibers. In this study we investigated the mechanism for immunologic rejection of normal C57BL/10 (B10) myoblasts transplanted into dystrophin-deficient mdx mice, an animal model of Duchenne muscular dystrophy. We found that mdx mice develop CTL specific for dystrophin itself, which were CD8 dominant and restricted by H-2Kb. We identified several antigenic peptides derived from dystrophin that bind to H-2Kb and are recognized by the mdx anti-B10 CTL. Immunologic tolerance against dystrophin was successfully induced by i.v. injection of these peptides before B10 myoblast transplantation, which resulted in sustained preservation of dystrophin-expressing myofibers in mdx mice. These results demonstrate that dystrophin is antigenic in dystrophin-deficient mice and that immunologic regimen would be necessary to achieve the persistent expression of introduced dystrophin in the muscles of dystrophin-deficient individuals.
Ohtsuka
Y
Y
Department of Immunology, Juntendo University School of Medicine, Tokyo, Japan.
Udaka
K
K
Yamashiro
Y
Y
Yagita
H
H
Okumura
K
K
D000595:Amino Acid Sequence; D000818:Animals; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology*; D017690:Cell Transplantation; D003602:Cytotoxicity, Immunologic; D016189:Dystrophin / Q000172:deficiency* / Q000235:genetics / Q000276:immunology*; D015316:Genetic Therapy; D006085:Graft Survival / Q000276:immunology; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D008969:Molecular Sequence Data; D018482:Muscle, Skeletal / Q000637:transplantation*; D009137:Muscular Dystrophy, Animal / Q000235:genetics* / Q000628:therapy*; D010455:Peptides / Q000276:immunology; D014181:Transplantation Immunology; D014184:Transplantation, Homologous
D016189:Dystrophin; D010455:Peptides