title: Early detection of epidermal dust-like particles in experimentally UV-induced lesions in patients with photosensitivity and lupus erythematosus.
abstract: Dust-like particles, producing a specific fine-speckled, epidermo-subepidermal direct immunofluorescence staining pattern, have been associated mainly with subacute cutaneous lupus erythematosus (LE). Under experimental conditions the appearance of immunoglobulins along the basement membrane in ultraviolet (UV) light-induced lesions has been reported as a late phenomenon. In this study, photoprovocations with UVA and UVB light were carried out in 16 photosensitive patients with discoid (n = 13), subacute cutaneous (n = 2) or systemic LE (n = 1) and serial biopsies from UV-induced lesions were processed for direct immunofluorescence. A specific, fine-speckled epidermal staining was detected within 7 to 14 days after UV provocation in 7/16 of the patients; in the majority of those patients associated with anti-SSA antibodies adn discoid LE without systemic manifestations of their disease.
authors.affiliation: Department of Dermatology, Karolinska Institute and Hospital, Stockholm, Sweden.
authors.forename: F
authors.initials: F
authors.lastname: Nyberg
authors.forename: C
authors.initials: C
authors.lastname: Skoglund
authors.forename: E
authors.initials: E
authors.lastname: Stephansson
mesh_terms: D000328:Adult; D000368:Aged; D000974:Antibodies, Antinuclear / Q000032:analysis / Q000276:immunology / Q000528:radiation effects; D015415:Biomarkers / Q000032:analysis*; D015922:Complement C1q / Q000032:analysis / Q000276:immunology / Q000528:radiation effects; D003176:Complement C3 / Q000276:immunology / Q000528:radiation effects; D004391:Dust / Q000032:analysis; D005260:Female; D005455:Fluorescent Antibody Technique; D006801:Humans; D007074:Immunoglobulin G / Q000032:analysis / Q000276:immunology / Q000528:radiation effects; D007075:Immunoglobulin M / Q000032:analysis / Q000276:immunology / Q000528:radiation effects; D008178:Lupus Erythematosus, Cutaneous / Q000150:complications / Q000276:immunology; D008179:Lupus Erythematosus, Discoid / Q000150:complications / Q000276:immunology; D008180:Lupus Erythematosus, Systemic / Q000150:complications / Q000276:immunology*; D008297:Male; D008875:Middle Aged; D010787:Photosensitivity Disorders / Q000150:complications / Q000276:immunology; D012867:Skin / Q000737:chemistry / Q000276:immunology* / Q000528:radiation effects*; D012871:Skin Diseases / Q000150:complications / Q000209:etiology / Q000276:immunology*; D014466:Ultraviolet Rays / Q000009:adverse effects
chemical_list: D000974:Antibodies, Antinuclear; D015415:Biomarkers; D003176:Complement C3; D004391:Dust; D007074:Immunoglobulin G; D007075:Immunoglobulin M; C035356:SS-A antibodies; D015922:Complement C1q