Water-deprived white-footed mice express c-fos on a day/night cycle graded according to the duration of deprivation.
Mammals respond to electrolyte and water imbalance by a variety of neural and endocrine mechanisms that regulate water and salt intake and loss. We used the expression of c-fos and Fos-related antigens to indicate neuronal activation in hypothalamic neurons of members of an outbred laboratory population of white-footed mice (Peromyscus leucopus) deprived of water for biologically reasonable periods of time (6-18 h). We examined Fos-like immunoreactivity (Fos-LIR) in the supraoptic nucleus (SON) and paraventricular nucleus (PVN). During the dark period, when these animals are normally active, 6 h of water deprivation produced near-maximal increases in the number of cells positive for Fos-LIR in the SON and PVN. In contrast, during the light period, when these mice are normally inactive and do not have access to water, 6 h of water deprivation only slightly affected Fos-LIR. During the day, it required as much as 12 h of water deprivation to produce increases in Fos-LIR cells approaching those achieved at night. Plasma osmolarity was directly related to the number of Fos-LIR cells. In addition, mice lost weight more rapidly at night than during the day when water-deprived, and also recovered that lost weight more rapidly when access to water was returned. Our results show (1) that biologically reasonable levels of water restriction (and resulting changes in blood osmolarity) induce changes in Fos-LIR in this wild mouse species, and (2) that these mice have a daily cycle of sensitivity to water deprivation that is demonstrated by both behavioral, psychological and immunohistological assessment of reactions to water deprivation.
Chae
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Department of Biology, The College of William and Mary, P.O. Box 8795, Williamsburg, VA 23187-8795, USA.
Heideman
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D000704:Analysis of Variance; D000818:Animals; D001127:Arginine Vasopressin / Q000032:analysis; D002940:Circadian Rhythm / Q000502:physiology*; D005260:Female; D007031:Hypothalamus / Q000378:metabolism*; D007124:Immunoenzyme Techniques; D008297:Male; D009419:Nerve Tissue Proteins / Q000096:biosynthesis*; D009474:Neurons / Q000737:chemistry; D009490:Neurosecretory Systems / Q000502:physiology*; D009994:Osmolar Concentration; D010121:Oxytocin / Q000032:analysis; D010542:Peromyscus; D016760:Proto-Oncogene Proteins c-fos / Q000096:biosynthesis*; D013997:Time Factors; D014868:Water Deprivation; D015431:Weight Loss / Q000502:physiology
D009419:Nerve Tissue Proteins; D016760:Proto-Oncogene Proteins c-fos; D001127:Arginine Vasopressin; D010121:Oxytocin