Synthesis of stereoselectively 13C/2H-doubly labeled DNA oligomers by the combined use of chemical and enzymatic reactions.
(2'S,5'S)-(1',2',3',4',5'-13C5;2',5'-2H2-thymidine, prepared via labeled ribosyl thymidine as described in the accompanying paper, has been successfully converted into isotopically labeled dA, dG, and dC using enzymatic transdeoxyribosylation reactions.
Ono
A
A
Department of Chemistry, Faculty of Science, Tokyo Metropolitan University, Japan.
Oogo
Y
Y
Tate
S
S
Ono
A
A
Kainosho
M
M
D002247:Carbon Isotopes; D004247:DNA / Q000138:chemical synthesis / Q000737:chemistry; D003903:Deuterium; D007202:Indicators and Reagents; D007553:Isotope Labeling / Q000379:methods; D009838:Oligodeoxyribonucleotides / Q000138:chemical synthesis* / Q000737:chemistry*; D013936:Thymidine
D002247:Carbon Isotopes; D007202:Indicators and Reagents; D009838:Oligodeoxyribonucleotides; D004247:DNA; D003903:Deuterium; D013936:Thymidine