Arginine vasopressin stimulates phospholipid methylation in cultured rat mesangial cells: possible role for PGE2 production.
Incorporation of [3H-methyl] groups into phospholipids and prostaglandin E2 (PGE2) production in cultured rat mesangial cells were examined in the presence and absence of arginine vasopressin (AVP). In cells stimulated with AVP, a rapid increase in the incorporation of [3H-methyl] group into phospholipids was observed within 1 min after stimulation. The [3H-methyl] group present in the phospholipids began to decline 2.5 min after stimulation. The production of PGE2 increased with AVP treatment, and the decline in methylated phospholipids paralleled the release of PGE2 in AVP-stimulated cells. The inhibition of phospholipid methylation by treatment with adenosyl-S-isobutyl mercaptan (SIBA) resulted in a marked decrease in AVP-stimulated PGE2 production. In order to determine the identity of the methylated phospholipids, [3H-methyl] incorporation into phosphatidylethanolamine derivatives was examined. In AVP-stimulated cells, an increase of [3H-methyl] labeled phosphatidylcholine and lysophosphatidylcholine was observed after stimulation with AVP, followed by an apparent increase of [3H-methyl] labeled lysophosphatidylcholine. These findings indicate that AVP stimulates phospholipid methylation in cultured rat mesangial cells and phosphatidylcholine, synthesized by a transmethylation pathway, may be a source for PGE2 production.
Nishida
M
M
Department of Pediatrics, Kyoto Prefectural University of Medicine, Japan.
Kawakatsu
H
H
Komatsu
H
H
Ishiwari
K
K
Tamai
M
M
Sawada
T
T
Hasegawa
T
T
Nishikawa
H
H
D000818:Animals; D000890:Anti-Infective Agents / Q000494:pharmacology; D001127:Arginine Vasopressin / Q000008:administration & dosage / Q000494:pharmacology*; D002478:Cells, Cultured; D003839:Deoxyadenosines / Q000494:pharmacology; D015232:Dinoprostone / Q000037:antagonists & inhibitors / Q000096:biosynthesis; D005920:Glomerular Mesangium / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D008244:Lysophosphatidylcholines / Q000378:metabolism; D008297:Male; D008745:Methylation / Q000187:drug effects; D010713:Phosphatidylcholines / Q000378:metabolism; D010743:Phospholipids / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley; D012076:Renal Agents / Q000008:administration & dosage / Q000494:pharmacology*; D013872:Thionucleosides / Q000494:pharmacology; D013997:Time Factors; D014316:Tritium
D000890:Anti-Infective Agents; D003839:Deoxyadenosines; D008244:Lysophosphatidylcholines; D010713:Phosphatidylcholines; D010743:Phospholipids; D012076:Renal Agents; D013872:Thionucleosides; D014316:Tritium; D001127:Arginine Vasopressin; C013919:5'-deoxy-5'-S-isobutylthioadenosine; D015232:Dinoprostone