Novel primers designed for microsatellite loci in Eucalyptus and identification by PCR fingerprints.
We found a novel PCR-primer which can be used for the identification on "elite-tree-selection". This primer was designed for selective hybridization at the both ends of microsatellite loci, which is well known as one of the most highpervariable region of DNA. After PCR-fingerprinting on five Eucalyptus species (E. globulus, E.citriodora, E.grandis, E. maidenii, E.bicostata), with our primer, DNA-polymorphism was observed all over the cases.
Matsuda
M
M
Biochemistry Section, Iwakuni Research Laboratory of Technology, Nippon Paper Industries Co. Ltd, Yamaguchi, Japan.
Kojima
E
E
Izumi
M
M
Murakami
K
K
D001483:Base Sequence; D016172:DNA Fingerprinting / Q000379:methods; D017931:DNA Primers; D018744:DNA, Plant / Q000737:chemistry; D018895:Microsatellite Repeats; D016133:Polymerase Chain Reaction / Q000379:methods; D013045:Species Specificity; D014197:Trees / Q000145:classification / Q000235:genetics*
D017931:DNA Primers; D018744:DNA, Plant