Molecular and genetic characterization of two anti-DNA antibodies derived from patients with systemic lupus erythematosus.
Anti-double stranded(ds) DNA antibody is one of markers of systemic lupus erythematosus (SLE). Two human monoclonal anti-DNA antibody-producing cell lines were established from two SLE patients. One cell line secreted IgG isotype antibody (KSUG) and the other secreted IgM isotype antibody (KSUN). The light chains of the two immunoglobulins were lambda chains. The nucleotide sequences for the immunoglobulin variable region genes of the two antibodies were determined and compared to germline sequences. The heavy and lambda light chains of KSUG were VH3 family and V lambda IIIb, respectively. The heavy and lambda light chains of KSUN were VH4 family and V lambda IX, respectively. Antibody KSUG, IgG isotype, showed somatic mutations, whereas KSUN, IgM isotype, used the germline gene without mutation. These findings reconfirm the current paradigms that IgM anti-DNA antibodies are produced by utilizing germline genes whereas IgG anti-DNA antibodies are produced by somatic mutations.
Song
Y W
YW
Department of Biochemistry, College of Medicine, Wonkwang University, Iksan, Korea. ysong@plaza.snu.ac.kr
Kim
H A
HA
Lee
E B
EB
Baek
H J
HJ
Kim
J K
JK
Chung
J H
JH
Chung
E S
ES
Hong
K M
KM
D000328:Adult; D000595:Amino Acid Sequence; D000974:Antibodies, Antinuclear / Q000235:genetics* / Q000276:immunology; D000911:Antibodies, Monoclonal; D001483:Base Sequence; D004247:DNA / Q000235:genetics* / Q000276:immunology; D017931:DNA Primers / Q000737:chemistry; D005260:Female; D006801:Humans; D006825:Hybridomas; D007074:Immunoglobulin G / Q000235:genetics; D007143:Immunoglobulin Heavy Chains / Q000235:genetics; D007075:Immunoglobulin M / Q000235:genetics; D007135:Immunoglobulin Variable Region / Q000235:genetics; D007146:Immunoglobulin lambda-Chains / Q000235:genetics; D008180:Lupus Erythematosus, Systemic / Q000235:genetics* / Q000276:immunology; D008875:Middle Aged; D008969:Molecular Sequence Data
D000974:Antibodies, Antinuclear; D000911:Antibodies, Monoclonal; D017931:DNA Primers; D007074:Immunoglobulin G; D007143:Immunoglobulin Heavy Chains; D007075:Immunoglobulin M; D007135:Immunoglobulin Variable Region; D007146:Immunoglobulin lambda-Chains; D004247:DNA