title: Induction of apoptosis reduces immunogenicity of human T-cell lines in mice.
abstract: Apoptotic cells, e.g. postinflammatory neutrophils, were reported to be engulfed by phagocytes without induction of an inflammatory response. We investigated the humoral immune response of BALB/c mice after repeated injection of viable or apoptotic human T cells. Following interleukin-2 (IL-2) deprivation, phytohaemagglutinin (PHA)/IL-2 expanded human T-cell lines were irradiated with UV-B light to induce apoptosis, confirmed by propidium iodide staining of Triton X-100-lysed cells. Indirect immunofluorescence was used to detect antilymphocyte antibodies 7 days after each injection. We found high levels of antilymphocyte antibodies in all animals immunized with viable T cells, whereas animals injected with apoptotic cells showed a significantly reduced humoral immune response. We conclude that apoptotic cells induce poor xenoreactive T-cell responses when compared with viable cells.
authors.affiliation: Institute for Clinical Immunology, Department of Medicine III, Erlangen, Germany.
authors.forename: B B
authors.initials: BB
authors.lastname: Ponner
authors.forename: C
authors.initials: C
authors.lastname: Stach
authors.forename: O
authors.initials: O
authors.lastname: Zoller
authors.forename: M
authors.initials: M
authors.lastname: Hagenhofer
authors.forename: R
authors.initials: R
authors.lastname: Voll
authors.forename: J R
authors.initials: JR
authors.lastname: Kalden
authors.forename: M
authors.initials: M
authors.lastname: Herrmann
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000276:immunology*; D002460:Cell Line; D002470:Cell Survival; D005260:Female; D006801:Humans; D051379:Mice; D008807:Mice, Inbred BALB C; D013601:T-Lymphocytes / Q000276:immunology* / Q000637:transplantation