title: Hypovolemia upregulates the expression of neuronal nitric oxide synthase gene in the paraventricular and supraoptic nuclei of rats.
abstract: We have examined the effects of isotonic hypovolemia on the expression of the neuronal nitric oxide synthase (nNOS) gene in the paraventricular (PVN) and supraoptic nuclei (SON) of the rat, using in situ hybridization histochemistry with a 35S-labelled oligodeoxynucleotide probe complementary to nNOS mRNA. Intraperitoneal (i.p.) administration of polyethylene glycol (PEG) (MW 4000, 20 ml/kg body weight) dissolved in 0.9% saline (20% w/v) induced isotonic hypovolemia. The expression of the nNOS gene in the PVN and SON 6 h after i.p. administration of PEG was increased significantly in comparison with controls. The dual staining for NADPH diaphorase activity and Fos-like immunoreactivity (Fos-LI) showed that at 3 and 6 h after i.p. administration of PEG, a subpopulation of NADPH diaphorase-positive cells in the PVN and SON exhibited nuclear Fos-LI. These results suggest that NO in the PVN and SON may be involved in the neuroendocrine and autonomic responses to non-osmotic hypovolemia.
authors.affiliation: Department of Physiology, School of Medicine, University of Occupational and Environmental Health, 1-1 Iseigaoka, Yahatanishi-ku, Kitakyushu 807-8555, Japan. yoichi@med.uoeh_u.ac.jp
authors.forename: Y
authors.initials: Y
authors.lastname: Ueta
authors.forename: A
authors.initials: A
authors.lastname: Levy
authors.forename: S L
authors.initials: SL
authors.lastname: Lightman
authors.forename: Y
authors.initials: Y
authors.lastname: Hara
authors.forename: R
authors.initials: R
authors.lastname: Serino
authors.forename: M
authors.initials: M
authors.lastname: Nomura
authors.forename: I
authors.initials: I
authors.lastname: Shibuya
authors.forename: Y
authors.initials: Y
authors.lastname: Hattori
authors.forename: H
authors.initials: H
authors.lastname: Yamashita
mesh_terms: D000818:Animals; D001798:Blood Proteins / Q000032:analysis; D005079:Excipients / Q000494:pharmacology; D015971:Gene Expression Regulation, Enzymologic / Q000502:physiology*; D017403:In Situ Hybridization; D008297:Male; D009252:NADPH Dehydrogenase / Q000378:metabolism; D009419:Nerve Tissue Proteins / Q000235:genetics / Q000378:metabolism; D019001:Nitric Oxide Synthase / Q000235:genetics* / Q000378:metabolism; D052248:Nitric Oxide Synthase Type I; D009994:Osmolar Concentration; D010286:Paraventricular Hypothalamic Nucleus / Q000201:enzymology / Q000503:physiopathology*; D011092:Polyethylene Glycols / Q000494:pharmacology; D016760:Proto-Oncogene Proteins c-fos / Q000235:genetics; D012333:RNA, Messenger / Q000032:analysis; D051381:Rats; D017207:Rats, Sprague-Dawley; D012769:Shock / Q000503:physiopathology*; D012964:Sodium / Q000097:blood; D013495:Supraoptic Nucleus / Q000201:enzymology / Q000503:physiopathology*
chemical_list: D001798:Blood Proteins; D005079:Excipients; D009419:Nerve Tissue Proteins; D016760:Proto-Oncogene Proteins c-fos; D012333:RNA, Messenger; D011092:Polyethylene Glycols; D012964:Sodium; D019001:Nitric Oxide Synthase; D052248:Nitric Oxide Synthase Type I; C496322:Nos1 protein, rat; D009252:NADPH Dehydrogenase