Molecular characterization of the phenylacetic acid catabolic pathway in Pseudomonas putida U: the phenylacetyl-CoA catabolon.
Fourteen different genes included in a DNA fragment of 18 kb are involved in the aerobic degradation of phenylacetic acid by Pseudomonas putida U. This catabolic pathway appears to be organized in three contiguous operons that contain the following functional units: (i) a transport system, (ii) a phenylacetic acid activating enzyme, (iii) a ring-hydroxylation complex, (iv) a ring-opening protein, (v) a beta-oxidation-like system, and (vi) two regulatory genes. This pathway constitutes the common part (core) of a complex functional unit (catabolon) integrated by several routes that catalyze the transformation of structurally related molecules into a common intermediate (phenylacetyl-CoA).
Olivera
E R
ER
Departamento de Bioquímica y Biología Molecular, Facultad de Veterinaria, Universidad de León, 24007 León, Spain.
Miñambres
B
B
García
B
B
Muñiz
C
C
Moreno
M A
MA
Ferrández
A
A
Díaz
E
E
García
J L
JL
Luengo
J M
JM
D000105:Acetyl Coenzyme A / Q000235:genetics / Q000378:metabolism*; D000595:Amino Acid Sequence; D001673:Biodegradation, Environmental; D008969:Molecular Sequence Data; D009154:Mutation; D010648:Phenylacetates / Q000378:metabolism*; D016958:Pseudomonas putida / Q000378:metabolism*; D016415:Sequence Alignment
D010648:Phenylacetates; D000105:Acetyl Coenzyme A; C037816:phenylacetyl-coenzyme A; C025136:phenylacetic acid