The effects of reductive dissociation of gastric mucin isolated in the presence of proteinase inhibitors.
Mucin was purified by the gel filtration method on columns with high porous molecular sives in buffers with SDS and proteinase inhibitors. The addition of proteinase inhibitors distinctly inhibited proteolytic activity. It was found that the obtained mucin, after disulphide-bound reduction, is dissociated to mucin subunits and N-glycosylated glycoprotein of molecular weight about 75 kDa. This protein has carbohydrate and amino acid composition different from high molecular fraction. The 75 kDa protein is strongly associated with high molecular mass mucin subunits and can be separated either during electrophoresis or fractionation in buffers with 2-mercaptoethanol.
Minkiewicz
I
I
Departament of General and Organic Chemistry, Medical Academy of Białystok.
Gindzieński
A
A
Kisiel
D G
DG
D000180:Acrylic Resins; D000596:Amino Acids / Q000032:analysis; D000818:Animals; D002021:Buffers; D002241:Carbohydrates / Q000032:analysis; D005591:Chemical Fractionation; D002845:Chromatography; D002850:Chromatography, Gel; D004220:Disulfides / Q000737:chemistry; D004591:Electrophoresis, Polyacrylamide Gel; D005752:Gastric Mucins / Q000032:analysis / Q000037:antagonists & inhibitors* / Q000302:isolation & purification / Q000494:pharmacology; D006023:Glycoproteins / Q000032:analysis; D008623:Mercaptoethanol / Q000737:chemistry; D008970:Molecular Weight; D010084:Oxidation-Reduction; D016062:Porosity; D011480:Protease Inhibitors / Q000494:pharmacology*; D012967:Sodium Dodecyl Sulfate / Q000737:chemistry; D013501:Surface-Active Agents / Q000737:chemistry; D013552:Swine
D000180:Acrylic Resins; D000596:Amino Acids; D002021:Buffers; D002241:Carbohydrates; D004220:Disulfides; D005752:Gastric Mucins; D006023:Glycoproteins; D011480:Protease Inhibitors; D013501:Surface-Active Agents; D012967:Sodium Dodecyl Sulfate; D008623:Mercaptoethanol; C053132:sephacryl S 500