The ability of human polymorphonuclear cells (PMNS) to release of soluble IL-6 receptor (IL-6sR) in response to different stimuli in vitro.
In the present work we have examined the release of soluble IL-6 receptor (IL-6sR) by polymorphonuclear cells (PMNs) in response to different stimuli: zymosan, ConA, PHA and LPS. We have also investigated the generation of IL-6sR by whole blood cells (WBC) under the same condition of the culture. IL-6sR secreted by PMNs and WBC were detected in cell-free supernatants by ELISA. Results of our investigation revealed that freshly isolated PMNs from healthy subjects exhibit a different ability to the secretion of IL-6sR in response to used stimuli. LPS showed the most significant effect on the IL-6sR secretion by PMNs and WBC as compared to the other inducers. The mean concentrations of IL-6sR released by PMNs were lower than concentrations of IL-6sR released by WBC. Measurements of the IL-6sR secretion by immunocompetent cells appear to be useful in the estimation of the cellular activity mediated by IL-6.
Jabłońska
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E
Department of Immunopathology, Medical Academy of Białystok.
Pietruska
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Z
D001402:B-Lymphocytes / Q000378:metabolism; D001773:Blood Cells / Q000187:drug effects / Q000378:metabolism; D002474:Cell-Free System; D003208:Concanavalin A / Q000494:pharmacology; D003470:Culture Media; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D008070:Lipopolysaccharides / Q000494:pharmacology; D008934:Mitogens / Q000494:pharmacology; D009504:Neutrophils / Q000187:drug effects / Q000378:metabolism*; D010835:Phytohemagglutinins / Q000494:pharmacology; D019947:Receptors, Interleukin-6 / Q000187:drug effects / Q000378:metabolism*; D013601:T-Lymphocytes / Q000378:metabolism; D015054:Zymosan / Q000494:pharmacology
D003470:Culture Media; D008070:Lipopolysaccharides; D008934:Mitogens; D010835:Phytohemagglutinins; D019947:Receptors, Interleukin-6; D003208:Concanavalin A; D015054:Zymosan