Analysis for the subcellular distribution of oligonucleotide/lipid complexes.
We synthesized a lipoglutamide having tetraethylene glycol tails (PEG lipid), and prepared a DNA/PEG lipid complex. Observation by a confocal laser scanning fluorescence microscope showed that the oligonucleotide/PEG lipid complex distributed in cytoplasmic region. On the other hand, a oligonucleotide/lipofectin complex distributed in nucleus. Interactions of the oligonucleotide complexes with isolated nucleus were also investigated, and mechanisms of the subcellular distributions were discussed.
Sato
T
T
Department of Biomolecular Engineering, Tokyo Institute of Technology, Yokohama, Japan.
Nishi
H
H
Okahata
Y
Y
Shoji
Y
Y
Shimada
J
J
D000818:Animals; D004247:DNA / Q000378:metabolism*; D004058:Diffusion; D004705:Endocytosis; D005026:Ethylene Glycols; D006367:HeLa Cells; D006801:Humans; D007700:Kinetics; D018613:Microscopy, Confocal / Q000379:methods; D008954:Models, Biological; D009838:Oligodeoxyribonucleotides / Q000493:pharmacokinetics*; D012474:Salmon; D013347:Subcellular Fractions / Q000378:metabolism / Q000648:ultrastructure; D013696:Temperature
D005026:Ethylene Glycols; D009838:Oligodeoxyribonucleotides; D004247:DNA