title: HPLC determination of photosynthetic pigments during greening of etiolated barley leaves. Evidence for the biosynthesis of chlorophyll a'.
abstract: The temporal evolution of pigment composition during greening of etiolated barley leaves was investigated by reversed-phase HPLC with particular attention to chlorophyll (Chl) a' (C13(2) epimer of Chl a), which had been detected by ourselves in photosystem (PS) I particles. At early stages of greening, the Chl a'/Chl a molar ratio rapidly increased to a level more than twice that in mature leaves, then gradually leveled off, accompanied by a growth of the Chl b/Chl a ratio, to the mature level. After 3 h of illumination, the temporal evolution of the Chl a'/Chl a molar ratio nearly paralleled that of the P700/Chl a ratio with a stoichiometry Chl a'/P700 approximately equal to 2: this strongly suggests that Chl a' is biosynthesized as a constituent of the PS I reaction center complex.
authors.affiliation: Institute of Industrial Science, University of Tokyo, Japan. watanabe@cc.iis.u-tokyo.ac.jp
authors.forename: A
authors.initials: A
authors.lastname: Nakamura
authors.forename: T
authors.initials: T
authors.lastname: Watanabe
mesh_terms: D002734:Chlorophyll / Q000031:analogs & derivatives* / Q000378:metabolism; D000077194:Chlorophyll A; D002736:Chloroplasts / Q000378:metabolism; D002851:Chromatography, High Pressure Liquid; D001467:Hordeum; D008027:Light; D045342:Light-Harvesting Protein Complexes; D010788:Photosynthesis; D045322:Photosynthetic Reaction Center Complex Proteins / Q000378:metabolism*; D013997:Time Factors
chemical_list: D045342:Light-Harvesting Protein Complexes; D045322:Photosynthetic Reaction Center Complex Proteins; D002734:Chlorophyll; C085815:chlorophyll a'; C001785:chlorophyll P 700; D000077194:Chlorophyll A