[Growth regulatory mechanisms of recombinant human interleukin-6 on human lung carcinoma cell lines].
OBJECTIVE
To detect the activity of interleukin-6 (rhIL-6) on human lung cancer and the influences of recombinant human IL-6 (rhIL-6) on the in vitro growth of high-metastatic human lung giant cell carcinoma cell line PG and low-metastatic human lung adenocarcinoma cell line PAa.


METHODS
The effects of rhIL-6 on the expressions of IL-6, IL-6R, c-myc, c-fos and c-jun mRNAs were analyzed by Northern blotting hybridization.


RESULTS
rhIL-6 could stimulate the in vitro growth of both PG and PAa cells, in a dose-and-time-dependent manner. Both PG and PAa cells were shown to express IL-6 and IL-6R identified by reverse transcription PCR analysis. Northern blotting hybridization revealed that rhIL-6 up-regulated mRNA levels of IL-6, IL-6R and c-myc rather than those of c-fos and c-jun.


CONCLUSIONS
These data suggest that rhIL-6 may promote the proliferation of human lung cancer cell lines PG and PAa, which might, at least partly, be related to the up-regulation of expressions of IL-6, IL-6R, c-myc gene transcript.
Fu
J
J
Department of Pathology, Beijing Medical University.
Wu
B
B
Zheng
J
J
D000230:Adenocarcinoma / Q000473:pathology*; D018286:Carcinoma, Giant Cell / Q000473:pathology*; D002455:Cell Division / Q000187:drug effects; D015972:Gene Expression Regulation, Neoplastic / Q000187:drug effects; D016259:Genes, myc; D006801:Humans; D015850:Interleukin-6 / Q000494:pharmacology*; D008175:Lung Neoplasms / Q000473:pathology*; D016271:Proto-Oncogene Proteins c-myc / Q000096:biosynthesis; D011994:Recombinant Proteins / Q000494:pharmacology; D014407:Tumor Cells, Cultured / Q000187:drug effects
D015850:Interleukin-6; D016271:Proto-Oncogene Proteins c-myc; D011994:Recombinant Proteins