title: Prevalence of influenza A virus (H1N1) antibodies in bovine sera.
abstract: An H1 subtype-specific indirect ELISA was used to determine the prevalence of influenza A virus antibodies in a retrospective study of 2,345 bovine sera in Minnesota. Twenty-seven percent of the samples tested were positive, 31% were low-positive and 42% were negative. The prevalence of antibody appeared to peak during the months of September to November and then again from February to March. A subset of the above samples was examined by hemagglutination-inhibition (HI) to confirm the ELISA results. A 92% correlation was found between the ELISA and hemagglutination-inhibition assay. Western blot analysis on a subset of ELISA positive sera (n = 50) confirmed the presence of antibodies to the nucleoprotein and H1 hemagglutinin protein of influenza A virus.
authors.affiliation: Department of Veterinary PathoBiology, College of Veterinary Medicine, University of Minnesota, St. Paul 55108, USA.
authors.forename: K
authors.initials: K
authors.lastname: Jones-Lang
authors.forename: M
authors.initials: M
authors.lastname: Ernst-Larson
authors.forename: B
authors.initials: B
authors.lastname: Lee
authors.forename: S M
authors.initials: SM
authors.lastname: Goyal
authors.forename: R
authors.initials: R
authors.lastname: Bey
mesh_terms: D000818:Animals; D000914:Antibodies, Viral / Q000097:blood*; D002417:Cattle; D002418:Cattle Diseases / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay / Q000662:veterinary; D006384:Hemagglutination; D053118:Influenza A Virus, H1N1 Subtype; D009980:Influenza A virus / Q000276:immunology*; D009976:Orthomyxoviridae Infections / Q000097:blood / Q000453:epidemiology / Q000276:immunology / Q000662:veterinary*; D015995:Prevalence; D012189:Retrospective Studies
chemical_list: D000914:Antibodies, Viral