Importance of the N-glycan in the V3 loop of HIV-1 envelope protein for CXCR-4- but not CCR-5-dependent fusion.
The V3 region of HIV-1 envelope protein possesses a single N-linked sugar chain, which is conserved in most HIV-1 strains. We studied its role in the life cycle of HIV-1 strains with different co-receptor usage. Removal of the glycan appeared to cause a marked reduction of CXCR-4- but not CCR-5-dependent virus entry. A basic amino acid substitution at the 11th position of V3 markedly compensated for the removal of the N-glycan. These results indicate that the N-glycan plays an important role for CXCR-4-dependent virus entry and that this role is exerted in a particular context of the peptide backbone.
Nakayama
E E
EE
Department of Viral Infection, Institute of Medical Science, University of Tokyo, Japan.
Shioda
T
T
Tatsumi
M
M
Xin
X
X
Yu
D
D
Ohgimoto
S
S
Kato
A
A
Sakai
Y
Y
Ohnishi
Y
Y
Nagai
Y
Y
D000595:Amino Acid Sequence; D019943:Amino Acid Substitution; D002461:Cell Line, Transformed; D015726:Giant Cells / Q000821:virology*; D006031:Glycosylation; D015699:HIV Envelope Protein gp120 / Q000235:genetics / Q000378:metabolism*; D015497:HIV-1 / Q000302:isolation & purification / Q000378:metabolism* / Q000502:physiology; D006801:Humans; D008969:Molecular Sequence Data; D016297:Mutagenesis, Site-Directed; D010446:Peptide Fragments / Q000235:genetics / Q000378:metabolism*; D011134:Polysaccharides / Q000502:physiology*; D019713:Receptors, CCR5 / Q000502:physiology*; D019718:Receptors, CXCR4 / Q000502:physiology*; D015728:Receptors, HIV / Q000502:physiology; D014779:Virus Replication
D015699:HIV Envelope Protein gp120; C059762:HIV envelope protein gp120 (305-321); D010446:Peptide Fragments; D011134:Polysaccharides; D019713:Receptors, CCR5; D019718:Receptors, CXCR4; D015728:Receptors, HIV