title: Age-dependent modulation of heparan sulfate structure and function.
abstract: Heparan sulfate interacts with growth factors, matrix components, effectors and modulators of enzymatic catalysis as well as with microbial proteins via sulfated oligosaccharide domains. Although a number of such domains have been characterized, little is known about the regulation of their formation in vivo. Here we show that the structure of human aorta heparan sulfate is gradually modulated during aging in a manner that gives rise to markedly enhanced binding to isoforms of platelet-derived growth factor A and B chains containing polybasic cell retention sequences. By contrast, the binding to fibroblast growth factor 2 is affected to a much lesser extent. The enhanced binding of aorta heparan sulfate to platelet-derived growth factor is suggested to be due to an age-dependent increase of GlcN 6-O-sulfation, resulting in increased abundance of the trisulfated L-iduronic acid (2-OSO3)-GlcNSO3(6-OSO3) disaccharide unit. Such units have been shown to hallmark the platelet-derived growth factor A chain-binding site in heparan sulfate.
authors.affiliation: Department of Medical Biochemistry and Microbiology, Uppsala University, S-75123 Uppsala, Sweden.
authors.forename: E
authors.initials: E
authors.lastname: Feyzi
authors.forename: T
authors.initials: T
authors.lastname: Saldeen
authors.forename: E
authors.initials: E
authors.lastname: Larsson
authors.forename: U
authors.initials: U
authors.lastname: Lindahl
authors.forename: M
authors.initials: M
authors.lastname: Salmivirta
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000375:Aging / Q000378:metabolism*; D001012:Aorta, Abdominal / Q000378:metabolism*; D002236:Carbohydrate Conformation; D005260:Female; D006133:Growth Substances / Q000378:metabolism; D006497:Heparan Sulfate / Q000737:chemistry / Q000378:metabolism*; D006801:Humans; D008297:Male; D008875:Middle Aged; D011485:Protein Binding
chemical_list: D006133:Growth Substances; D006497:Heparan Sulfate