Gene regulation by decoy approach (I): synthesis and properties of photo-crosslinked oligonucleotides.
To explore gene regulation by double stranded oligonucleotides as decoy molecules for transcriptional factors, oligodeoxyribonucleotides (ODN) and the complementary oligo(deoxyribonucleoside phosphorothioate)s (OPT) were photo-crosslinked by use of psoralen. UV melting curves showed that the thermal stability of crosslinked duplexes increased compared with that of non-crosslinked ones. CD spectra showed that the photo-crosslinked duplexes formed B-DNA structure. In addition, cross-linked duplexes were resistant against digestion by snake venom phosphodiesterase. These results suggest that the photo-crosslinked duplexes have desirable characteristics as decoy molecules.
Iwase
R
R
Department of Polymer Science and Engineering, Kyoto Institute of Technology, Japan.
Namba
M
M
Yamaoka
T
T
Murakami
A
A
D001483:Base Sequence; D002942:Circular Dichroism; D003432:Cross-Linking Reagents; D004247:DNA / Q000138:chemical synthesis / Q000737:chemistry*; D011564:Furocoumarins; D005786:Gene Expression Regulation; D009690:Nucleic Acid Conformation; D009838:Oligodeoxyribonucleotides / Q000138:chemical synthesis* / Q000737:chemistry; D010777:Photochemistry
D003432:Cross-Linking Reagents; D011564:Furocoumarins; D009838:Oligodeoxyribonucleotides; D004247:DNA