title: HPLC on Chiralcel OJ-R for enantiomer separation and analysis of ketoprofen, from horse plasma, as the 9-aminophenanthrene derivative.
abstract: Racemic ketoprofen is a non-steroidal anti-inflammatory drug used to treat musculoskeletal and colic conditions in horses. The enantioselective chiral inversion of ketoprofen administered to horses has been studied by use of cellulose tris(4-methylbenzoate), also known as Chiralcel OJ-R, as chiral stationary phase; acetonitrile - 0.02 M perchlorate buffer (pH 2.0)-methanol, 60:15:25 (v/v/v) was used as mobile phase. Before chromatography, to effect adequate chiral interaction with the chiral stationary phase ketoprofen was derivatized with 9-aminophenanthrene, under acid conditions, after solid-phase (C18) extraction and then liquid-liquid extraction, to ensure effective removal of endogenous plasma materials. The 9-aminophenanthrene derivative of S-ibuprofen was used as internal standard. The enantiomers of ketoprofen were separated to baseline (Rs = 6.44, alpha = 1.76) within a short analysis time. The results indicate that the bio-inversion of R-ketoprofen to the S isomer is significant in equine species. However, considerable differences in pharmacokinetic parameters were observed, indicating large inter-animal variation.
authors.affiliation: Biological and Medical Research, MBC-03, King Faisal Specialist Hospital and Research Centre, Riyadh, Saudi Arabia.
authors.forename: H Y
authors.initials: HY
authors.lastname: Aboul-Enein
authors.forename: A
authors.initials: A
authors.lastname: Van Overbeke
authors.forename: G
authors.initials: G
authors.lastname: Vander Weken
authors.forename: W
authors.initials: W
authors.lastname: Baeyens
authors.forename: H
authors.initials: H
authors.lastname: Oda
authors.forename: P
authors.initials: P
authors.lastname: Deprez
authors.forename: A
authors.initials: A
authors.lastname: De Kruif
mesh_terms: D000818:Animals; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000097:blood* / Q000302:isolation & purification; D001565:Benzoates; D001711:Biotransformation; D002482:Cellulose / Q000031:analogs & derivatives; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D005456:Fluorescent Dyes; D006736:Horses; D007052:Ibuprofen / Q000097:blood / Q000302:isolation & purification; D007660:Ketoprofen / Q000097:blood* / Q000302:isolation & purification*; D008297:Male; D010616:Phenanthrenes; D013237:Stereoisomerism
chemical_list: D000894:Anti-Inflammatory Agents, Non-Steroidal; D001565:Benzoates; D005456:Fluorescent Dyes; D010616:Phenanthrenes; C090722:tris(4-methylbenzoate)cellulose; C041014:9-aminophenanthrene; D002482:Cellulose; D007660:Ketoprofen; D007052:Ibuprofen