Induction of single-strand breaks in lymphocyte DNA of rats exposed to hyperoxia.
A modified technique of alkaline filter elution was used to evaluate single-strand breaks (SSB) as a measure of DNA lesions in lymphocytes of male Wistar rats exposed to 60 or 80 vol% oxygen, over 23 h/day and for 6 or 5 days, respectively. An acceleration of elution was observed with samples from experiments at both increased levels of oxygen. With proteinase K treatment, a twofold increase in elution rates after exposure to 60 vol% oxygen and a threefold increase at 80 vol% oxygen were indicative of increased occurrence of SSB. This is explained by an involvement of reactive oxygen species in DNA damage under elevated oxygen levels.
Vangala
R R
RR
Kritzler
K
K
Schöch
G
G
Topp
H
H
D000818:Animals; D018736:DNA Adducts / Q000378:metabolism; D004249:DNA Damage; D018496:Hyperoxia / Q000378:metabolism*; D008214:Lymphocytes / Q000187:drug effects* / Q000378:metabolism; D008297:Male; D009152:Mutagenicity Tests; D010100:Oxygen / Q000633:toxicity*; D051381:Rats; D017208:Rats, Wistar
D018736:DNA Adducts; D010100:Oxygen