title: Plastome mutator-induced alterations arise in Oenothera chloroplast DNA through template slippage.
abstract: The plastome mutator of Oenothera hookeri strain Johansen causes deletions and duplications at target sites defined by direct repeats in the plastid genome. Previous studies characterized the mutations long after they had occurred and could not discriminate between the possibilities that the plastome mutator acted through unequal homologous recombination or template slippage. From the known hotspots, the rRNA spacer in the large inverted repeat was chosen for this study because it contains both direct and indirect repeats. Identical deletions were recovered from independently derived plants; the altered regions were always flanked by direct repeats. The regions in which the deletions occurred have the potential to form secondary structures that would stabilize the intervening sequence. Of the two affected regions, the one with the stronger potential secondary structure was altered more frequently. Because no duplication products or inversions were recovered, it is proposed that the plastome mutator acts through template slippage rather than through a recombination mechanism.
authors.affiliation: Department of Botany and Plant Pathology, Michigan State University, East Lansing, Michigan 48824, USA.
authors.forename: L L
authors.initials: LL
authors.lastname: Stoike
authors.forename: B B
authors.initials: BB
authors.lastname: Sears
mesh_terms: D000483:Alleles; D001483:Base Sequence; D002736:Chloroplasts / Q000235:genetics*; D018744:DNA, Plant / Q000737:chemistry*; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation; D010944:Plants / Q000235:genetics*; D017384:Sequence Deletion; D013698:Templates, Genetic
chemical_list: D018744:DNA, Plant