title: Non-enzymatic hydrolyses of aryl esters of thymidine 3'-monophosphate as probes for the rate-determining step in DNA hydrolysis.
abstract: In order to shed light on the rate-limiting step in DNA hydrolysis, non-enzymatic hydrolyses of aryl esters of thymidine 3'-monophosphate have been kinetically examined. In alkaline solutions, these dinucleotide analogues were hydrolyzed far faster than was thymidylyl(3'-5')thymidine (TpT), indicating that the departure of the leaving group from the phosphorus atom is rate-limiting. The CeIV- and CeIV/PrIII combination-mediated hydrolyses of these analogues were also faster than the corresponding hydrolysis of the dinucleotide.
authors.affiliation: Department of Chemistry and Biotechnology, Graduate School of Engineering, University of Tokyo, Japan.
authors.forename: N
authors.initials: N
authors.lastname: Takeda
authors.forename: Y
authors.initials: Y
authors.lastname: Okada
authors.forename: M
authors.initials: M
authors.lastname: Yashiro
authors.forename: M
authors.initials: M
authors.lastname: Komiyama
mesh_terms: D002563:Cerium; D004247:DNA / Q000737:chemistry*; D015226:Dinucleoside Phosphates / Q000737:chemistry*; D006868:Hydrolysis; D007202:Indicators and Reagents; D007700:Kinetics; D011221:Praseodymium; D013329:Structure-Activity Relationship
chemical_list: D015226:Dinucleoside Phosphates; D007202:Indicators and Reagents; C017758:thymidylyl-(3'-5')-thymidine; D002563:Cerium; D004247:DNA; D011221:Praseodymium