Ligand- and nuclear factor-dependent change in hydrophobicity of thyroid hormone beta1 receptor.
An aqueous two-phase partitioning assay was performed using in vitro translated human thyroid hormone beta1 receptor (TRbeta1). Wild-type TRbeta1 was less hydrophobic in the presence of both triiodothyronine (T3) and nuclear extract. This reflects a conformational change, or change in electrostatic properties, of the TRbeta1-nuclear factor complex as a result of T3 binding. Mutant TRbeta1s with reduced T3 binding affinity required a higher concentration of T3 for the shift of hydrophobicity, and a mutant without T3 binding activity did not show any shift, even in the presence of 1 mM T3. The unique mutant receptor, R243Q, has impaired transcriptional function despite virtually normal binding affinity for T3. When this mutant was examined in this assay, the shift of hydrophobicity was significantly impaired even in the presence of both nuclear extract and a high concentration of T3. Nuclear extract of COS1 cells did not affect the T3-binding affinity of R243Q. These results indicate that the R243Q mutant has impaired a ligand-dependent conformational change and interaction with nuclear factor(s). Inability of R243Q to interact normally with nuclear factor(s) may explain, in part, the molecular mechanism of discordance between ligand binding and transactivation function of this mutant.
Sakurai
A
A
Department of Geriatrics, Endocrinology and Metabolism, Shinshu University School of Medicine, Asahi, Matsumoto, Japan.
Katai
M
M
Miyamoto
T
T
Ichikawa
K
K
Hashizume
K
K
D000818:Animals; D002412:Cations / Q000494:pharmacology; D002467:Cell Nucleus / Q000502:physiology*; D055598:Chemical Phenomena; D002627:Chemistry, Physical; D004247:DNA / Q000235:genetics; D007700:Kinetics; D008024:Ligands; D008297:Male; D014176:Protein Biosynthesis; D011487:Protein Conformation; D051381:Rats; D017208:Rats, Wistar; D011988:Receptors, Thyroid Hormone / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D014284:Triiodothyronine / Q000494:pharmacology
D002412:Cations; D008024:Ligands; D011988:Receptors, Thyroid Hormone; D014284:Triiodothyronine; D004247:DNA