Structure and chromosomal localization of the mouse bombesin receptor subtype 3 gene.
Bombesin (BN)-like peptides/neurotransmitters mediate a broad range of physiological funtions in the gastrointestinal tract and the central nervous system through binding to their specific, high-affinity mammalian bombesin receptors. This family of heptahelical, G-protein coupled receptors includes the gastrin-releasing peptide receptor (GRP-R, or bb2), neuromedin B receptor (NMB-R, or bb1), and the bombesin receptor subtype 3 (BRS-3, or bb3). The tissue distribution of BRS-3 is quite dissimilar compared to the other two BN receptors, GRP-R and NMB-R, and a natural ligand for BRS-3 is currently unknown. Nothing is known about mechanisms regulating BRS-3 gene expression and possible association with disease. To gain insight into the underlying structure and chromosomal localization of the BRS-3 genes, bacteriophage P1 genomic clones, harboring the genes for the human and mouse BRS-3, respectively, were isolated and their structure and chromosomal localizations determined. The protein-coding region of both genes is divided into three exons and spans approximately 5kb. The loci of the BRS-3 genes were mapped to a syntenic region of the human (Xq25) and mouse (XA7.1-7.2) X-chromosome, respectively. The structural data of the BRS-3 genes derived from this study will permit future investigations of the mechanisms regulating their expression.
Weber
H C
HC
Section of Gastroenterology, Boston University School of Medicine, 88 East Newton Street, Boston, MA 02118-2393, USA. christian.weber@bmc.org
Hampton
L L
LL
Jensen
R T
RT
Battey
J F
JF
D000595:Amino Acid Sequence; D000818:Animals; D017101:Bacteriophage P1 / Q000235:genetics; D001483:Base Sequence; D002874:Chromosome Mapping; D003001:Cloning, Molecular; D005786:Gene Expression Regulation / Q000235:genetics; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D051379:Mice; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D018004:Receptors, Bombesin / Q000737:chemistry*; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D014960:X Chromosome / Q000235:genetics
D018004:Receptors, Bombesin; C107120:bombesin receptor subtype 3