title: Screening system for urease inhibitors using 13C-NMR.
abstract: Urease inhibitors are candidate drugs to treat infection with the human pathogen, Helicobacter pylori, which produces a potent urease [urea amidohydrolase; EC 3.5.1.5]. We developed a screening system based on 13C-NMR measurement of the time course of decrease in the signal of 13C-urea in the presence of urease. The effect on urease activity of known inhibitors, hydroxamic acids, L-ascorbic acid, 2,2'-dipyridyl disulfide and ninhydrin, was speedily and conveniently measured by this method.
authors.affiliation: Department of Medicinal Chemistry, Meiji College of Pharmacy, Tokyo, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Chiyoda
authors.forename: K
authors.initials: K
authors.lastname: Iida
authors.forename: K
authors.initials: K
authors.lastname: Takatori
authors.forename: M
authors.initials: M
authors.lastname: Kajiwara
mesh_terms: D001667:Binding, Competitive; D001944:Breath Tests / Q000379:methods*; D004791:Enzyme Inhibitors / Q000494:pharmacology*; D000431:Ethanol / Q000737:chemistry; D016480:Helicobacter pylori / Q000187:drug effects* / Q000201:enzymology*; D006877:Hydroxamic Acids / Q000494:pharmacology*; D007202:Indicators and Reagents; D009682:Magnetic Resonance Spectroscopy / Q000379:methods*; D009555:Ninhydrin; D013379:Substrate Specificity; D014510:Urease / Q000037:antagonists & inhibitors*
chemical_list: D004791:Enzyme Inhibitors; D006877:Hydroxamic Acids; D007202:Indicators and Reagents; D000431:Ethanol; C006358:acetohydroxamic acid; D014510:Urease; D009555:Ninhydrin