title: The determination of pamidronate in pharmaceutical preparations by ion-pair liquid chromatography after derivatization with phenylisothiocyanate.
abstract: An analytical method was developed for the determination of pamidronate [(3-amino-1-hydroxypropylidene)bisphosphonate] by ion-pair liquid chromatography. The analyte was derivatized with phenylisothiocyanate into an UV-absorbing derivative. The reaction product was cleaned-up by a double ion-pair extraction and treated with hydrogen peroxide prior to injection. Both, the detection limit and the lower limit of quantification of pamidronate in water were 0.1 microgram ml-1 disodium pamidronate. The intra-day precision was 3% for a 5-microgram ml-1 pamidronate standard solution and the inter-day precision 6% for a 3-microgram ml-1 solution. The method was applied in the quality control of pamidronate injection concentrates and tablets.
authors.affiliation: Department of Clinical Pharmacy and Toxicology, Leiden University Hospital, The Netherlands.
authors.forename: R W
authors.initials: RW
authors.lastname: Sparidans
authors.forename: J
authors.initials: J
authors.lastname: den Hartigh
authors.forename: W M
authors.initials: WM
authors.lastname: Ramp-Koopmanschap
authors.forename: R H
authors.initials: RH
authors.lastname: Langebroek
authors.forename: P
authors.initials: P
authors.lastname: Vermeij
mesh_terms: D002851:Chromatography, High Pressure Liquid; D002853:Chromatography, Liquid; D004164:Diphosphonates / Q000032:analysis*; D007202:Indicators and Reagents; D017879:Isothiocyanates; D000077268:Pamidronate; D013861:Thiocyanates
chemical_list: D004164:Diphosphonates; D007202:Indicators and Reagents; D017879:Isothiocyanates; D013861:Thiocyanates; C005441:phenylisothiocyanate; D000077268:Pamidronate