A function for the AP3 coat complex in synaptic vesicle formation from endosomes.
Synaptic vesicles can be coated in vitro in a reaction that is ARF-, ATP-, and temperature-dependent and requires synaptic vesicle membrane proteins. The coat is largely made up of the heterotetrameric complex, adaptor protein 3, recently implicated in Golgi-to-vacuole traffic in yeast. Depletion of AP3 from brain cytosol inhibits small vesicle formation from PC12 endosomes in vitro. Budding from washed membranes can be reconstituted with purified AP3 and recombinant ARF1. We conclude that AP3 coating is involved in at least one pathway of small vesicle formation from endosomes.
Faúndez
V
V
Department of Biochemistry and Biophysics and Hormone Research Institute, University of California, San Francisco 94143-0534, USA.
Horng
J T
JT
Kelly
R B
RB
D020823:ADP-Ribosylation Factor 1; D020727:ADP-Ribosylation Factors; D033963:Adaptor Protein Complex 3; D033965:Adaptor Protein Complex alpha Subunits; D033966:Adaptor Protein Complex beta Subunits; D033942:Adaptor Proteins, Vesicular Transport; D000255:Adenosine Triphosphate / Q000502:physiology; D000818:Animals; D001665:Binding Sites; D001921:Brain; D003600:Cytosol / Q000378:metabolism; D011992:Endosomes / Q000502:physiology*; D005260:Female; D019204:GTP-Binding Proteins / Q000032:analysis / Q000502:physiology*; D016244:Guanosine 5'-O-(3-Thiotriphosphate); D006160:Guanosine Triphosphate; D008565:Membrane Proteins / Q000032:analysis / Q000502:physiology*; D034041:Monomeric Clathrin Assembly Proteins; D009419:Nerve Tissue Proteins / Q000032:analysis / Q000502:physiology*; D016716:PC12 Cells; D010750:Phosphoproteins / Q000032:analysis / Q000502:physiology*; D011402:Pronase / Q000037:antagonists & inhibitors / Q000494:pharmacology; D011480:Protease Inhibitors / Q000494:pharmacology; D051381:Rats; D017207:Rats, Sprague-Dawley; D013572:Synaptic Vesicles / Q000737:chemistry / Q000502:physiology*
C095597:AP3B2 protein, human; D033963:Adaptor Protein Complex 3; D033965:Adaptor Protein Complex alpha Subunits; D033966:Adaptor Protein Complex beta Subunits; D033942:Adaptor Proteins, Vesicular Transport; D008565:Membrane Proteins; D034041:Monomeric Clathrin Assembly Proteins; D009419:Nerve Tissue Proteins; D010750:Phosphoproteins; D011480:Protease Inhibitors; C439023:clathrin assembly protein AP180; D016244:Guanosine 5'-O-(3-Thiotriphosphate); D006160:Guanosine Triphosphate; D000255:Adenosine Triphosphate; D011402:Pronase; D019204:GTP-Binding Proteins; D020823:ADP-Ribosylation Factor 1; D020727:ADP-Ribosylation Factors