title: A recombinant fragment of Helicobacter pylori CagA affects proliferation of human cells.
abstract: The outcome of H. pylori infectins depends on proliferation of various host cells, including lymphocytes, monocytes and epithelial cells. In this study we showed that a recombinant fusion protein carrying an immunodominant region of H. pylori CagA antigen affected the proliferation of human cells. The rCagA inhibited PHA-driven T cell proliferation but enhanced the growth of epithelial HeLa cells, especially in the presence of granulocyte macrophage colony stimulating factor (GM-CSF). When THP-1 monocytes and Kato-3 epithelial cells from metastasis of gastric carcinoma were stimulated with GM-CSF, they were also susceptible to the inhibitory effect of rCagA. These results confirmed our earlier suggestion on the inhibition of T cell function by H. pylori CagA protein. However, antiproliferative activity of CagA antigen appears to be not restricted to T lymphocytes but modulatory effect of this protein seems to depend on the cell type.
authors.affiliation: Department of Infectious Biology, University of Lodz, Poland.
authors.forename: W
authors.initials: W
authors.lastname: Rudnicka
authors.forename: A
authors.initials: A
authors.lastname: Covacci
authors.forename: T
authors.initials: T
authors.lastname: Wadstrom
authors.forename: M
authors.initials: M
authors.lastname: Chmiela
mesh_terms: D000942:Antigens, Bacterial; D001426:Bacterial Proteins / Q000737:chemistry / Q000494:pharmacology*; D002455:Cell Division / Q000187:drug effects; D002460:Cell Line; D006367:HeLa Cells / Q000166:cytology / Q000187:drug effects*; D016480:Helicobacter pylori / Q000378:metabolism*; D006801:Humans; D009000:Monocytes / Q000166:cytology / Q000187:drug effects*; D010446:Peptide Fragments / Q000494:pharmacology*; D011993:Recombinant Fusion Proteins / Q000494:pharmacology*; D013601:T-Lymphocytes / Q000166:cytology / Q000187:drug effects*
chemical_list: D000942:Antigens, Bacterial; D001426:Bacterial Proteins; D010446:Peptide Fragments; D011993:Recombinant Fusion Proteins; C080866:cagA protein, Helicobacter pylori