title: Identification and molecular analysis of lbpBA, which encodes the two-component meningococcal lactoferrin receptor.
abstract: We identified lbpB, encoding the lipoprotein component of the meningococcal lactoferrin receptor. An LbpB mutant was unable to acquire Fe from lactoferrin and exhibits decreased surface binding to lactoferrin. Primer extension and reverse transcription-PCR analysis indicate that lbpB and lbpA are cotranscribed on a polycistronic Fe-repressible mRNA.
authors.affiliation: Department of Microbiology and Immunology, University of Oklahoma Health Sciences Center, Oklahoma City, Oklahoma 73103, USA. llewis@rex.uokhsc.edu
authors.forename: L A
authors.initials: LA
authors.lastname: Lewis
authors.forename: K
authors.initials: K
authors.lastname: Rohde
authors.forename: M
authors.initials: M
authors.lastname: Gipson
authors.forename: B
authors.initials: B
authors.lastname: Behrens
authors.forename: E
authors.initials: E
authors.lastname: Gray
authors.forename: S I
authors.initials: SI
authors.lastname: Toth
authors.forename: B A
authors.initials: BA
authors.lastname: Roe
authors.forename: D W
authors.initials: DW
authors.lastname: Dyer
mesh_terms: D000595:Amino Acid Sequence; D001425:Bacterial Outer Membrane Proteins / Q000235:genetics*; D001426:Bacterial Proteins / Q000096:biosynthesis / Q000235:genetics*; D001483:Base Sequence; D002352:Carrier Proteins / Q000235:genetics; D003001:Cloning, Molecular; D015964:Gene Expression Regulation, Bacterial; D005798:Genes, Bacterial; D007501:Iron / Q000378:metabolism; D033862:Iron-Binding Proteins; D007781:Lactoferrin / Q000378:metabolism*; D008074:Lipoproteins / Q000096:biosynthesis / Q000235:genetics*; D008969:Molecular Sequence Data; D016296:Mutagenesis; D009345:Neisseria meningitidis / Q000235:genetics*; D012329:RNA, Bacterial / Q000096:biosynthesis; D012333:RNA, Messenger / Q000096:biosynthesis; D011956:Receptors, Cell Surface / Q000235:genetics*; D017422:Sequence Analysis, DNA; D014158:Transcription, Genetic; D033863:Transferrin-Binding Proteins
chemical_list: D001425:Bacterial Outer Membrane Proteins; D001426:Bacterial Proteins; D002352:Carrier Proteins; D033862:Iron-Binding Proteins; C112566:LBPA protein, Neisseria meningitidis; D008074:Lipoproteins; D012329:RNA, Bacterial; D012333:RNA, Messenger; D011956:Receptors, Cell Surface; D033863:Transferrin-Binding Proteins; D007501:Iron; D007781:Lactoferrin