title: PTH-responsive osteoblast nuclear matrix architectural transcription factor binds to the rat type I collagen promoter.
abstract: In connective tissue, cell structure contributes to type I collagen expression. Differences in osteoblast microarchitecture may account for the two distinct cis elements regulating basal expression, in vivo and in vitro, of the rat type I collagen alpha1(I) polypeptide chain (COL1A1). The COL1A1 promoter conformation may be the penultimate culmination of osteoblast structure. Architectural transcription factors bind to the minor groove of AT-rich DNA and bend it, altering interactions between other trans-acting proteins. Similarly, nuclear matrix (NM) proteins bind to the minor groove of AT-rich matrix-attachment regions, regulating transcription by altering DNA structure. We propose that osteoblast NM architectural transcription factors link cell structure to promoter geometry and COL1A1 transcription. Our objective was to identify potential osteoblast NM architectural transcription factors near the in vitro and in vivo regulatory regions of the rat COL1A1 promoter. Nuclear protein-promoter interactions were analyzed by gel shift analysis and related techniques. NM extracts were derived from rat osteosarcoma cells and from rat bone. The NM protein, NMP4, and a soluble nuclear protein, NP, both bound to two homologous poly(dT) elements within the COL1A1 in vitro regulatory region and proximal to the in vivo regulatory element. These proteins bound within the minor groove and bent the DNA. Parathyroid hormone increased NP/NMP4 binding to both poly(dT) elements and decreased COL1A1 mRNA in the osteosarcoma cells. NP/NMP4-COL1A1 promoter interactions may represent a molecular pathway by which osteoblast structure is coupled to COL1A1 expression.
authors.affiliation: Department of Oral Biology, Indiana University School of Dentistry, Indianapolis 46202, USA.
authors.forename: M
authors.initials: M
authors.lastname: Alvarez
authors.forename: P
authors.initials: P
authors.lastname: Thunyakitpisal
authors.forename: P
authors.initials: P
authors.lastname: Morrison
authors.forename: J
authors.initials: J
authors.lastname: Onyia
authors.forename: J
authors.initials: J
authors.lastname: Hock
authors.forename: J P
authors.initials: JP
authors.lastname: Bidwell
mesh_terms: D000818:Animals; D034961:Antigens, Nuclear; D001665:Binding Sites; D001842:Bone and Bones / Q000187:drug effects / Q000378:metabolism; D003094:Collagen / Q000235:genetics* / Q000378:metabolism; D004268:DNA-Binding Proteins / Q000737:chemistry / Q000378:metabolism; D008970:Molecular Weight; D034521:Nuclear Matrix-Associated Proteins; D009687:Nuclear Proteins / Q000737:chemistry / Q000378:metabolism*; D010006:Osteoblasts / Q000187:drug effects* / Q000378:metabolism; D010281:Parathyroid Hormone / Q000494:pharmacology*; D011071:Poly T / Q000378:metabolism; D011401:Promoter Regions, Genetic; D011485:Protein Binding; D051381:Rats; D014157:Transcription Factors / Q000737:chemistry / Q000378:metabolism*; D014407:Tumor Cells, Cultured; D014466:Ultraviolet Rays
chemical_list: D034961:Antigens, Nuclear; D004268:DNA-Binding Proteins; D034521:Nuclear Matrix-Associated Proteins; D009687:Nuclear Proteins; D010281:Parathyroid Hormone; D014157:Transcription Factors; C440548:Zfp384 protein, rat; D011071:Poly T; D003094:Collagen