Isolation and structural characterisation of rhamnogalacturonan oligomers generated by controlled acid hydrolysis of sugar-beet pulp.
Controlled acid hydrolysis was applied to a deesterified beet pulp and the resulting soluble fraction was fractionated on a Biorad AG 1X8 column eluted by ammonium acetate pH 6 from 0.05 to 2 M. Eight retained fractions were obtained, containing almost exclusively GalA and Rha. Three types of oligomers could be identified: homogalacturonans, of which mono-,di- and tri-GalA were isolated as individual components, and two series of rhamnogalacturonan (RG) oligomers. One RG oligomer, isolated after ion-exchange chromatography, was identified as alpha-D-GalAp-(1-->2)-alpha-L-Rhap-(1-->4)-alpha-D-GalA p-(1-->2)-L-Rhap. The major peak contained oligomers of dp 6 to more than 20, of which dp 6 to 16 could be isolated on Bio-Gel P-6 + P-4. NMR of the oligomers of dp 6 to 10 showed the following structure: alpha-D-GalAp-(1[-->2)-alpha-L-Rhap-(1-->4)-alpha-D-GalA p-(1]n-->2)-L-Rhap. A second, quantitatively minor, series of RG oligomers eluted at higher ionic strength. These oligomers, which could be hydrolysed by RG-hydrolase and RG-lyase, were based on the alternating RG structure. Their non-reducing end was GalA, susceptible to hydrolysis by RG-galacturonohydrolase, and their reducing end might have more than one consecutive GalA.
Renard
C M
CM
Laboratoire de Biochimie et Technologie des Glucides, I.N.R.A., Nantes, France. renard@nantes.inra.fr
Lahaye
M
M
Mutter
M
M
Voragen
F G
FG
Thibault
J F
JF
D000143:Acids; D002240:Carbohydrate Sequence; D002247:Carbon Isotopes; D019663:Chenopodiaceae / Q000737:chemistry*; D006868:Hydrolysis; D008969:Molecular Sequence Data; D019906:Nuclear Magnetic Resonance, Biomolecular; D009844:Oligosaccharides / Q000737:chemistry*; D010368:Pectins / Q000737:chemistry*; D011522:Protons
D000143:Acids; D002247:Carbon Isotopes; D009844:Oligosaccharides; D011522:Protons; C042491:rhamnogalacturonan I; D010368:Pectins