Multiple domains for initiator binding proteins TFII-I and YY-1 are present in the initiator and upstream regions of the rat XDH/XO TATA-less promoter.
We previously reported that the TATA-less rat xanthine dehydrogenase/oxidase (XDH/XO) promoter is organized with multiple initiator elements (Inr 1, 2, 3 and 4). Additionally, we identified six factor binding footprints in the upstream region of this promoter (FP 1-FP 6), two of which (FP 2 and FP 4) we showed to be C/EBP binding sites. In this report we continue our characterization of the XDH/XO promoter, detailing other cis elements which comprise the Inr and upstream binding factors. Interestingly, multiple binding domains for known initiator binding proteins, YY-1 and USF-related factor/TFII-I, have been identified which potentially play an important role in transcription initiation.
Clark
M P
MP
Nashville VA Medical Center, Department of Medicine, School of Medicine, Vanderbilt University, Nashville, TN 37237, USA.
Chow
C W
CW
Rinaldo
J E
JE
Chalkley
R
R
D000818:Animals; D001665:Binding Sites; D022762:CCAAT-Enhancer-Binding Proteins; D004268:DNA-Binding Proteins / Q000378:metabolism*; D050981:Erythroid-Specific DNA-Binding Factors; D051863:Host Cell Factor C1; D051839:NFI Transcription Factors; D009687:Nuclear Proteins / Q000378:metabolism; D050811:Octamer Transcription Factor-1; D011401:Promoter Regions, Genetic; D016271:Proto-Oncogene Proteins c-myc / Q000378:metabolism; D051381:Rats; D016385:TATA Box; D014157:Transcription Factors / Q000378:metabolism*; D051720:Upstream Stimulatory Factors; D014968:Xanthine Dehydrogenase / Q000235:genetics*; D051840:Y-Box-Binding Protein 1
D022762:CCAAT-Enhancer-Binding Proteins; D004268:DNA-Binding Proteins; D050981:Erythroid-Specific DNA-Binding Factors; D051863:Host Cell Factor C1; D051839:NFI Transcription Factors; D009687:Nuclear Proteins; D050811:Octamer Transcription Factor-1; C494168:Pou2f1 protein, rat; D016271:Proto-Oncogene Proteins c-myc; D014157:Transcription Factors; D051720:Upstream Stimulatory Factors; C497182:Usf1 protein, rat; D051840:Y-Box-Binding Protein 1; C486169:Ybx1 protein, rat; D014968:Xanthine Dehydrogenase