title: Comparative genomic hybridization: technical development and cytogenetic aspects for routine use in clinical laboratories.
abstract: Comparative genomic hybridization (CGH) offers a new global approach for detection of chromosomal material imbalances of the entire genome in a single experiment without cell culture. In this paper, we discuss the technical development and the cytogenetic aspects of CGH in a clinical laboratory. Based only on the visual inspection of CGH metaphase spreads, the correct identification of numerical and structural anomalies are reported. No commercial image analysis software was required in these experiments. We have demonstrated that this new technology can be set up easily for routine use in a clinical cytogenetics laboratory.
authors.affiliation: Service de Biochimie Génétique, Hôpital Robert Debré AP-HP, Paris, France.
authors.forename: J M
authors.initials: JM
authors.lastname: Lapierre
authors.forename: V
authors.initials: V
authors.lastname: Cacheux
authors.forename: F
authors.initials: F
authors.lastname: Da Silva
authors.forename: N
authors.initials: N
authors.lastname: Collot
authors.forename: N
authors.initials: N
authors.lastname: Hervy
authors.forename: J
authors.initials: J
authors.lastname: Wiss
authors.forename: G
authors.initials: G
authors.lastname: Tachdjian
mesh_terms: D000782:Aneuploidy; D001667:Binding, Competitive; D002869:Chromosome Aberrations / Q000175:diagnosis* / Q000235:genetics; D025063:Chromosome Disorders; D002877:Chromosomes, Human / Q000235:genetics / Q000648:ultrastructure; D015342:DNA Probes; D003955:Diagnostic Tests, Routine / Q000379:methods; D005260:Female; D006801:Humans; D017404:In Situ Hybridization, Fluorescence / Q000295:instrumentation / Q000379:methods*; D008297:Male; D008677:Metaphase; D008856:Microscopy, Fluorescence / Q000295:instrumentation; D009369:Neoplasms / Q000235:genetics* / Q000473:pathology
chemical_list: D015342:DNA Probes