Production and characterization of a monoclonal antibody able to discriminate galectin-1 from galectin-2 and galectin-3.
Antisera raised against galectin-1 exhibit crossreactivities with other galectins or related molecules. In order to overcome this problem, a monoclonal antibody to human brain galectin-1 was obtained by selecting clones without reactivity toward galectin-3. This mAb specifically bound galectin-1 of various animal origins but neither galectin-2 nor galectin-3. Western-blotting analysis of soluble human brain extracts after 2D gel electrophoresis revealed only the two most acidic isoforms of galectin-1. The ability of this mAb to bind galectin-1/asialofetuin complexes indicates that its epitope is not localized in the carbohydrate recognition domain of galectin-1. This particularity induces with efficiency its monospecificity.
Cornillot
J D
JD
Laboratoire de Biochimie et Technologie des Protéines (EA 1625), Université Paris Nord, UFR Santé, Médecine et Biologie Humaine, Bobigny, France.
Pontet
M
M
Dupuy
C
C
Chadli
A
A
Caron
M
M
Joubert-Caron
R
R
Bourin
P
P
Bladier
D
D
D000595:Amino Acid Sequence; D000911:Antibodies, Monoclonal; D000918:Antibody Specificity; D000943:Antigens, Differentiation / Q000032:analysis / Q000737:chemistry* / Q000276:immunology; D001923:Brain Chemistry; D003429:Cross Reactions; D004797:Enzyme-Linked Immunosorbent Assay; D037483:Galectin 1; D037501:Galectin 2; D037502:Galectin 3; D006388:Hemagglutinins / Q000032:analysis / Q000737:chemistry* / Q000276:immunology; D006801:Humans; D037102:Lectins / Q000737:chemistry; D008969:Molecular Sequence Data; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid
D000911:Antibodies, Monoclonal; D000943:Antigens, Differentiation; D037483:Galectin 1; D037501:Galectin 2; D037502:Galectin 3; D006388:Hemagglutinins; D037102:Lectins