Inhibition of eukaryote serine/threonine-specific protein kinases by piceatannol.
The protein tyrosine kinase (PTK) inhibitor piceatannol is also an inhibitor of the rat liver cyclic AMP-dependent protein kinase (PKA) catalytic subunit (cAK), rat brain Ca(2+)- and phospholipid-dependent protein kinase C (PKC), avian gizzard Ca(2+)-calmodulin-dependent myosin light chain kinae (MLCK), and of wheat embryo Ca(2+)-dependent protein kinase (CDPK) (IC50 values 3, 8, 12, and 19 microM, respectively). However, a number of piceatannol-related compounds with fewer or no phenolic hydroxy substituents are inactive or very poor inhibitors of these serine/threonine protein kinases. Similarly, the PTK inhibitor ellagic acid is a potent inhibitor of cAK and of PKC (IC50 values 2 and 8 microM, respectively), whereas the non-phenolic perylene is ineffective as a protein kinase inhibitor. Ellagic acid is a competitive inhibitor of both cAK and of PKC but piceatannol inhibits these enzymes in a fashion that is competitive and non-competitive, respectively. Interaction with calmodulin may contribute to the inhibition of MLCK and CDPK by piceatannol.
Wang
B H
BH
Baker Medical Research Institute, Prahran, Victoria, Australia.
Lu
Z X
ZX
Polya
G M
GM
D000818:Animals; D001921:Brain / Q000201:enzymology; D004610:Ellagic Acid / Q000494:pharmacology; D004791:Enzyme Inhibitors / Q000494:pharmacology*; D017346:Protein Serine-Threonine Kinases / Q000037:antagonists & inhibitors*; D051381:Rats; D013267:Stilbenes / Q000494:pharmacology*
D004791:Enzyme Inhibitors; D013267:Stilbenes; D004610:Ellagic Acid; C041525:3,3',4,5'-tetrahydroxystilbene; D017346:Protein Serine-Threonine Kinases