title: Release of interleukin-2 induced by a major antigenic outer membrane protein of Shigella dysenteriae type 1 in natural infection.
abstract: An antigen specific modulation of peripheral blood lymphocyte function was examined in a patient-based study of Shigella dysenteriae type 1 infection. Interleukin-2 (IL-2) production by the peripheral blood mononuclear cells (PBMC) from Shigella-infected patients was correlated with the expression of host cellular immune responses. To evaluate the role of a 57 kD major antigenic outer membrane protein of S. dysenteriae 1 in the proliferation of PBMC and the production of IL-2, the in vitro blastogenic transformation assay was employed. The magnitude of the response was monitored morphologically as well as by the proliferation of the IL-2 dependent CTLL-2 cell line. The proliferation of the IL-2 dependent CTLL-2 cell line against PBMC culture fluids after exposure to the major antigen reflected the participation of functionally active T-lymphocytes in shigellosis patients. The precise quantitation of IL-2 concentration in such lymphocyte culture supernatants by immunoassay showed substantial production of IL-2.
authors.affiliation: Division of Immunology, National Institute of Cholera and Enteric Diseases, Beliaghata, Calcutta, India.
authors.forename: S
authors.initials: S
authors.lastname: Chakrabarti
authors.forename: A K
authors.initials: AK
authors.lastname: Sinha
mesh_terms: D000911:Antibodies, Monoclonal; D000942:Antigens, Bacterial / Q000737:chemistry / Q000276:immunology*; D000954:Antigens, Surface / Q000276:immunology; D001425:Bacterial Outer Membrane Proteins / Q000737:chemistry / Q000276:immunology*; D002460:Cell Line; D004396:Coloring Agents / Q000737:chemistry; D004405:Dysentery, Bacillary / Q000276:immunology* / Q000503:physiopathology; D004591:Electrophoresis, Polyacrylamide Gel; D006801:Humans; D007124:Immunoenzyme Techniques; D007376:Interleukin-2 / Q000096:biosynthesis* / Q000097:blood; D007963:Leukocytes, Mononuclear / Q000166:cytology / Q000276:immunology*; D008213:Lymphocyte Activation; D012762:Shigella dysenteriae / Q000276:immunology* / Q000378:metabolism; D013602:T-Lymphocytes, Cytotoxic / Q000166:cytology / Q000276:immunology; D013778:Tetrazolium Salts / Q000737:chemistry; D013844:Thiazoles / Q000737:chemistry
chemical_list: D000911:Antibodies, Monoclonal; D000942:Antigens, Bacterial; D000954:Antigens, Surface; D001425:Bacterial Outer Membrane Proteins; D004396:Coloring Agents; D007376:Interleukin-2; D013778:Tetrazolium Salts; D013844:Thiazoles; C022616:thiazolyl blue