Bacterial penetration through Resolut resorbable membrane in vitro. An histological and scanning electron microscopic study.
The aim of this study was to evaluate the characteristics of resorption and the pattern of bacterial collonisation of polyglycolic and polylactic resorbable membranes under controlled experimental conditions. A removable cobalt-chromium device was applied to the lower jaw of 5 students for a period of 4 weeks. 8 composite resin chambers were glued to the device, 4 on each side of the mouth. A small piece of PLA/PGA membrane separated the composite chambers into 2 parts. The subjects wore the devices 24 h a day, except for the time necessary for oral hygiene procedures, during which time, the structure was submerged in a 0.2% chlorexidine solution. Every week, 2 of the chambers were removed; one was processed for scanning electron microscopy, to be observed both on the external and internal surface, and the other one for light microscopy examination. Both the electron microscopic and histologic observations showed a progressive increase in the plaque layer on the external surface of the membrane during the period of observation. The light microscopy showed an early invasion of the membrane, starting about 1 week after the exposure. On all the 3- and 4-week specimens, a complete bacterial invasion over the whole thickness of the membrane was visible. After 3 weeks of plaque accumulation, bacterial colonisation of the inner portion of the membrane was detectable in all the specimens. At 3-weeks, we observed in the light microscopy group in 4 out of 5 specimens, a large reduction of the thickness of the material and small voids in the membrane structure. In 1 specimen, the membrane was no more detectable. Only in 5 specimens of the 4-week group was the membrane still recognizable, though reduced to fragments. In conclusion, once exposed to the oral cavity, the PLA/PGA membranes start to resorb in the early stages: this process concludes itself between the 3rd and 4th weeks of exposure. However, the particular conditions of the experimental design nevertheless classify this study as an in vitro, more than as an in vivo experimentation.
Simion
M
M
Department of Dentistry, University of Milan, Italy.
Maglione
M
M
Iamoni
F
F
Scarano
A
A
Piattelli
A
A
Salvato
A
A
D000328:Adult; D001422:Bacterial Adhesion; D001672:Biocompatible Materials; D003773:Dental Plaque / Q000382:microbiology*; D016557:Guided Tissue Regeneration, Periodontal; D006801:Humans; D020007:Implants, Experimental / Q000382:microbiology*; D008567:Membranes, Artificial; D008855:Microscopy, Electron, Scanning; D011098:Polyglactin 910
D001672:Biocompatible Materials; D008567:Membranes, Artificial; C094810:Resolut; D011098:Polyglactin 910