Cytotoxicity of xenobiotics and expression of glutathione-S-transferases in immortalised rat hepatocyte cell lines.
1. Immortalised rat hepatocyte cell lines are more sensitive to the cytotoxicity of 1-chloro-2,4-dinitrobenzene and ethacrynic acid than primary cultures of hepatocytes. 2. Class alpha glutathione S-transferases are not expressed in immortalised hepatocyte cell lines. Class pi glutathione S-transferase expression is elevated in the immortalised cell lines compared with freshly isolated hepatocytes, but it is not as high as in the HTC rat hepatoma cell line. 3. Immortalised hepatocyte cell lines may provide a sensitive model system for detecting cytotoxicity associated with xenobiotics which are detoxified by glutathione S-transferases.
Anderson
K
K
Bioengineering Unit, Strathclyde University, Wolfson Centre, Glasgow, UK.
Andrews
R
R
Yin
L
L
McLeod
R
R
MacDonald
C
C
Hayes
J D
JD
Grant
M H
MH
D000818:Animals; D006528:Carcinoma, Hepatocellular; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D004137:Dinitrochlorobenzene / Q000633:toxicity*; D004976:Ethacrynic Acid / Q000633:toxicity*; D005982:Glutathione Transferase / Q000096:biosynthesis*; D015151:Immunoblotting; D007527:Isoenzymes / Q000096:biosynthesis*; D008099:Liver / Q000166:cytology / Q000187:drug effects* / Q000201:enzymology; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley; D013778:Tetrazolium Salts; D013844:Thiazoles; D018675:Toxicity Tests; D015262:Xenobiotics / Q000633:toxicity*
D004137:Dinitrochlorobenzene; D007527:Isoenzymes; D013778:Tetrazolium Salts; D013844:Thiazoles; D015262:Xenobiotics; D005982:Glutathione Transferase; C022616:thiazolyl blue; D004976:Ethacrynic Acid