title: Solid-phase extraction and high-performance liquid chromatography procedures for the analysis of paralytic shellfish toxins.
abstract: Paralytic shellfish poisoning (PSP) toxins are produced by certain dinoflagellate species such as Gymnodinium catenatum and Alexandrium tamarensis, during certain periods of the year influenced by several environmental factors, affecting the aquaculture industry and mainly bivalve molluscs. HPLC with fluorescence detection is a powerful analytical technique for the analysis of such toxins; several HPLC alternatives have been developed in order to improve the liquid chromatographic analysis, but due to the complexity of the sample matrix, important work has been focused recently on the clean-up of samples prior to HPLC analysis. Solid-phase extraction procedures offer advantages for this clean-up. In this work we focus on the study of three different clean-up methods prior to HPLC with fluorescence detection analysis of PSP toxin present in contaminated mussel samples; by spiking uncontaminated mussel samples with two different PSP toxin standards and by calculating the recovery values for these experiments. These recoveries must be taken into account in order to quantify the exact amount of PSP toxins present in the contaminated samples.
authors.affiliation: Departamento de Química Analítica y Alimentaria, Facultad de Ciencias (CUVI), Universidad de Vigo, Spain.
authors.forename: J M
authors.initials: JM
authors.lastname: Leão
authors.forename: A
authors.initials: A
authors.lastname: Gago
authors.forename: J A
authors.initials: JA
authors.lastname: Rodríguez-Vázquez
authors.forename: E C
authors.initials: EC
authors.lastname: Aguete
authors.forename: M M
authors.initials: MM
authors.lastname: Omil
authors.forename: M
authors.initials: M
authors.lastname: Comesaña
mesh_terms: D000818:Animals; D049872:Bivalvia / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D005504:Food Analysis / Q000379:methods*; D012530:Saxitoxin / Q000031:analogs & derivatives / Q000032:analysis*
chemical_list: D012530:Saxitoxin; C034903:decarbamylsaxitoxin