title: Differentiation of a porcine reproductive and respiratory syndrome virus vaccine strain from North American field strains by restriction fragment length polymorphism analysis of ORF 5.
abstract: The suitability of restriction fragment length polymorphism (RFLP) analysis for differentiating a porcine reproductive and respiratory syndrome virus (PRRSV) vaccine strain from other North American field strains was investigated. Open reading frame 5 nucleotide sequence data of the vaccine virus, its parent strain VR-2332, and 22 other strains of PRRSV included in this study indicated that 3 restriction enzyme gel patterns characterize the vaccine virus and the parent strain genotype. The combined 3 RFLP patterns differentiate the vaccine and parent virus from other PRRSV strains. This test will be a valuable tool in epidemiologic studies and will be useful in identifying individual strains in cases of multistrain PRRSV infections.
authors.affiliation: Virology Swine Research Unit, National Animal Disease Center, USDA, Agricultural Research Service, Ames, IA 50010, USA.
authors.forename: R D
authors.initials: RD
authors.lastname: Wesley
authors.forename: W L
authors.initials: WL
authors.lastname: Mengeling
authors.forename: K M
authors.initials: KM
authors.lastname: Lager
authors.forename: D F
authors.initials: DF
authors.lastname: Clouser
authors.forename: J G
authors.initials: JG
authors.lastname: Landgraf
authors.forename: M L
authors.initials: ML
authors.lastname: Frey
mesh_terms: D000818:Animals; D001483:Base Sequence; D003062:Codon; D004262:DNA Restriction Enzymes; D009656:North America; D016366:Open Reading Frames; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length; D019318:Porcine Reproductive and Respiratory Syndrome / Q000276:immunology* / Q000517:prevention & control; D019316:Porcine respiratory and reproductive syndrome virus / Q000145:classification / Q000235:genetics* / Q000276:immunology*; D012367:RNA, Viral / Q000032:analysis; D013552:Swine; D014765:Viral Vaccines
chemical_list: D003062:Codon; D012367:RNA, Viral; D014765:Viral Vaccines; D004262:DNA Restriction Enzymes