Biochemical and immunocytochemical properties of peroxisomes and mitochondria in bovine chromaffin cells.
The biochemical and immunocytochemical properties of peroxisomal and mitochondrial beta-oxidation enzymes in bovine adrenal chromaffin cells were investigated. Peroxisomes were detectable by immunofluorescence staining using antibodies against acyl-CoA oxidase, peroxisomal 3-ketoacyl-CoA thiolase and catalase. The mitochondria were abundantly stained with antibody against mitochondrial 3-ketoacyl-CoA thiolase. The biosynthesis and intracellular processing of acyl-CoA oxidase and the peroxisomal 3-ketoacyl-CoA thiolase was slower than that in fibroblasts. The peroxisomal beta-oxidation activities shown by [1-14C] lignoceric acid oxidation were slightly lower than those in fibroblasts, whereas the mitochondrial beta-oxidation activities shown by [1-14C] palmitic acid oxidation were almost identical to those in fibroblasts. Adrenal chromaffin cells are useful materials for investigating the peroxisomal and mitochondrial metabolism of autonomic neurons and may contribute to the clarification of neuronal dysfunction in peroxisomal and mitochondrial disorders.
Suzuki
Y
Y
Department of Pediatrics, Gifu University School of Medicine, Japan. ysuz@cc.gifu-u.ac.jp
Lee
K
K
Shimozawa
N
N
Orii
T
T
Kondo
N
N
D000102:Acetyl-CoA C-Acyltransferase / Q000378:metabolism; D042962:Acyl-CoA Oxidase; D000313:Adrenal Medulla / Q000166:cytology; D000818:Animals; D002417:Cattle; D019439:Chromaffin Cells / Q000737:chemistry* / Q000201:enzymology*; D005227:Fatty Acids / Q000032:analysis / Q000378:metabolism; D019084:Fluorescent Antibody Technique, Indirect; D008830:Microbodies / Q000737:chemistry / Q000201:enzymology*; D008928:Mitochondria / Q000737:chemistry / Q000201:enzymology*; D010084:Oxidation-Reduction; D010088:Oxidoreductases / Q000378:metabolism; D019308:Palmitic Acid / Q000032:analysis / Q000378:metabolism; D013564:Sympathetic Nervous System / Q000166:cytology
D005227:Fatty Acids; D019308:Palmitic Acid; D010088:Oxidoreductases; D042962:Acyl-CoA Oxidase; D000102:Acetyl-CoA C-Acyltransferase; C010210:lignoceric acid