Preparation of DNA-chitosan columns and their applications: binding of carcinogens to the column.
DNA, either native or denatured, can be immobilized by complexing with chitosan. The DNA-chitosan, which is insoluble in aqueous media, contains DNA at about 50% in weight. The DNA in the complex is accessible by enzymes and reagents. Carcinogenic heterocyclic amines, having affinity to DNA, can be adsorbed to the DNA-chitosan. The DNA-chitosan complex can be homogeneously dispersed in celite powder, and the resultant DNA-chitosan-celite is a material suitable to construct an immobilized DNA-column. The column was shown to be effective in concentrating heterocyclic amines.
Hayatsu
H
H
Faculty of Pharmaceutical Sciences, Okayama University, Japan.
Tanaka
Y
Y
Negishi
K
K
D000327:Adsorption; D000588:Amines / Q000737:chemistry* / Q000302:isolation & purification; D002273:Carcinogens / Q000737:chemistry* / Q000302:isolation & purification; D002686:Chitin / Q000031:analogs & derivatives*; D048271:Chitosan; D002846:Chromatography, Affinity / Q000379:methods; D004247:DNA; D007692:Diatomaceous Earth; D011131:Polyribonucleotides; D011489:Protein Denaturation; D012313:RNA
D000588:Amines; D002273:Carcinogens; D011131:Polyribonucleotides; D002686:Chitin; D007692:Diatomaceous Earth; D012313:RNA; D004247:DNA; D048271:Chitosan