title: Primary structure of porcine spleen ribonuclease: sequence homology.
abstract: The primary structure of porcine spleen RNase (RNase Psp1) was investigated as a mean of assessing the structure-function relationship of base non-specific ribonucleases of animal origin. N-terminal analysis of RNase Psp1 yielded three N-terminal sequences. These peptides were separated by gel-filtration on Superdex 75HR, after reduction and S-carboxymethylation of RNase Psp1. Determination of the amino-acid sequence of these peptides indicated that the RNase Psp1 preparation consisted of three peptides having 20 (RCM RNase Psp1 pep1), 15 (RCM RNase Psp1 pep2), and 164 (RCM RNase Psp1 pro) amino-acid residues, respectively. It possessed two unique segments containing most of the active site amino-acid residues of the RNases of the RNase T2 family. The alignment of these three peptides in RNase Psp1 was determined by comparison with the other enzymes in the RNase T2 family. The overall results showed that RCM RNase Psp1 pep1 and RCM RNase Psp1 pep2 are derived from the N-terminal and C-terminal regions of RNase Psp1, respectively, probably by processing by some protease. The molecular mass of the protein moiety of RNase Psp1 was 23235 Da.
authors.affiliation: Ohashi Hospital, Toho University, Meguro, Japan.
authors.forename: A
authors.initials: A
authors.lastname: Kusano
authors.forename: M
authors.initials: M
authors.lastname: Iwama
authors.forename: A
authors.initials: A
authors.lastname: Sanda
authors.forename: K
authors.initials: K
authors.lastname: Suwa
authors.forename: E
authors.initials: E
authors.lastname: Nakaizumi
authors.forename: Y
authors.initials: Y
authors.lastname: Nakatani
authors.forename: H
authors.initials: H
authors.lastname: Ohkawa
authors.forename: K
authors.initials: K
authors.lastname: Ohgi
authors.forename: M
authors.initials: M
authors.lastname: Irie
mesh_terms: D000595:Amino Acid Sequence; D000596:Amino Acids / Q000032:analysis; D000818:Animals; D004722:Endoribonucleases / Q000737:chemistry / Q000235:genetics; D008969:Molecular Sequence Data; D008970:Molecular Weight; D012260:Ribonucleases / Q000737:chemistry* / Q000235:genetics* / Q000302:isolation & purification; D017386:Sequence Homology, Amino Acid; D013045:Species Specificity; D013154:Spleen / Q000201:enzymology*; D013552:Swine
chemical_list: D000596:Amino Acids; D004722:Endoribonucleases; D012260:Ribonucleases; C022543:ribonuclease T(2)