A dynein light chain of sea urchin sperm flagella is a homolog of mouse Tctex 1, which is encoded by a gene of the t complex sterility locus.
The outer-arm dynein of sea urchin sperm flagella contains six light chains with molecular masses of 23.2, 20.8, 12.3, 11.5, 10.4 and 9. 3kDa. We have cloned a cDNA for the 12.3kDa polypeptide (light chain 3) and found that this protein is highly homologous to mouse Tctex1, a protein encoded by a member of the multigene family in the t complex region that is involved in male sterility and the development of the germ cells. Tctex1 has recently been shown to be homologous to a light chain of cytoplasmic dynein. Therefore, the cytoplasmic dynein light chain has been implicated in the mechanism for the transmission ratio distortion (meiotic drive) that is characteristic of t haplotypes in mice. Our present finding, however, indicates that axonemal light chain 3 must be considered equally important.
Kagami
O
O
Laboratory of Cell Communication, National Institute for Basic Biology, Okazaki 444, Japan. kagami@nibb.ac.jp
Gotoh
M
M
Makino
Y
Y
Mohri
H
H
Kamiya
R
R
Ogawa
K
K
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D056404:Chaperonin Containing TCP-1; D018833:Chaperonins / Q000235:genetics*; D017124:Conserved Sequence; D018076:DNA, Complementary / Q000737:chemistry / Q000235:genetics; D004398:Dyneins / Q000737:chemistry / Q000235:genetics*; D005796:Genes / Q000235:genetics*; D007248:Infertility, Male / Q000235:genetics*; D047908:Intracellular Signaling Peptides and Proteins; D008297:Male; D051379:Mice; D008869:Microtubule-Associated Proteins; D008969:Molecular Sequence Data; D009687:Nuclear Proteins / Q000235:genetics*; D012617:Sea Urchins / Q000737:chemistry / Q000166:cytology / Q000235:genetics*; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid; D013082:Sperm Tail / Q000737:chemistry*; D013094:Spermatozoa / Q000737:chemistry; D044767:Ubiquitin-Protein Ligases; D056427:t-Complex Genome Region
D018076:DNA, Complementary; C533386:Dynlt1b protein, mouse; D047908:Intracellular Signaling Peptides and Proteins; D008869:Microtubule-Associated Proteins; D009687:Nuclear Proteins; C116196:PPP1R11 protein, human; D044767:Ubiquitin-Protein Ligases; D056404:Chaperonin Containing TCP-1; D018833:Chaperonins; D004398:Dyneins