PP7, a plant phosphatase representing a novel evolutionary branch of eukaryotic protein Ser/Thr phosphatases.
We describe a novel protein Ser/Thr phosphatase from Arabidopsis thaliana, PP7, which is only 27-32% identical in amino acid sequence to the known phosphatases and is the most divergent member of the PPP (PP1/2A/2B) family for today. Some structural features suggest more close relationship of PP7 to the PP5/rdgC subfamily. PP7 contains all of the residues essential for the phosphatase activity and possesses three major insertions in its presumable C-terminal subdomain, which suggest its unique regulation and/or optimisation of its structure for interaction with specific substrates or regulators. A phosphatase structurally related to PP7 is expressed in rice. PP7 conservation between mono- and dicotyledonous plants may point to its essential role in the plant cell.
Andreeva
A V
AV
Research School of Biological and Molecular Sciences, Oxford Brookes University, UK.
Evans
D E
DE
Hawes
C R
CR
Bennett
N
N
Kutuzov
M A
MA
D000595:Amino Acid Sequence; D001483:Base Sequence; D018744:DNA, Plant / Q000032:analysis / Q000235:genetics; D019143:Evolution, Molecular; D008969:Molecular Sequence Data; D010749:Phosphoprotein Phosphatases / Q000235:genetics*; D010940:Plant Proteins / Q000235:genetics*; D010944:Plants / Q000201:enzymology* / Q000235:genetics
D018744:DNA, Plant; D010940:Plant Proteins; D010749:Phosphoprotein Phosphatases