DNA labeling reagent containing carbodiimide.
We have synthesized a number of fluorescent compounds having a carbodiimide function and tested if it can be used to label DNA. They all reacted well with DNA in a very short time. Some of them showed different fluorescent characteristics when they bound to DNA such as band shift or change in fluorescent intensity.
Masuda
G
G
Nisshinbo Tokyo Research Center, Tokyo, Japan.
Matsumoto
K
K
Suzuki
O
O
D002234:Carbodiimides; D004247:DNA / Q000737:chemistry*; D005456:Fluorescent Dyes / Q000138:chemical synthesis*; D007202:Indicators and Reagents; D008958:Models, Molecular; D008968:Molecular Conformation; D013050:Spectrometry, Fluorescence / Q000379:methods
D002234:Carbodiimides; D005456:Fluorescent Dyes; D007202:Indicators and Reagents; D004247:DNA