pICln can regulate swelling-induced Cl- currents in either layer of rabbit ciliary epithelium.
Swelling-induced Cl- currents were investigated in freshly prepared non-pigmented epithelial (NPE) and pigmented epithelial (PE) cells of the rabbit ciliary body using the whole-cell patch clamp technique. Exposure of both NPE and PE cells to hypotonic stress induced Cl- currents that exhibited outward rectification and were insensitive to Ca+2. We found that swelling-induced Cl- currents in PE cell are observed shortly after isolation. The swelling-induced Cl- current showed little or no inactivation at positive membrane voltages and was sensitive to 100 microM NPPB and 100 microM DIDS. Injection of cRNA encoded rabbit pICln into Xenopus oocytes produced an outwardly rectifying Cl- current displaying features consistent with the swelling-induced Cl- current in epithelium. pICln is ubiquitous in the ciliary epithelium. It participates in the equilibration of short term tonicity alterations, a phenomenon underlying mechanisms with larger and slower amplitudes for aqueous secretion by these cells.
Chen
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Department of Ophthalmology and Visual Science, Yale School of Medicine, New Haven, Connecticut 06520-8061, USA.
Wan
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Sears
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D000818:Animals; D018118:Chloride Channels / Q000235:genetics / Q000378:metabolism*; D002712:Chlorides / Q000378:metabolism*; D002924:Ciliary Body / Q000033:anatomy & histology / Q000378:metabolism*; D004848:Epithelium / Q000033:anatomy & histology / Q000378:metabolism; D015870:Gene Expression; D066298:In Vitro Techniques; D007473:Ion Channels; D017136:Ion Transport; D008564:Membrane Potentials; D009865:Oocytes / Q000378:metabolism; D009997:Osmotic Pressure; D018408:Patch-Clamp Techniques; D010857:Pigment Epithelium of Eye / Q000033:anatomy & histology / Q000378:metabolism; D018075:RNA, Complementary / Q000235:genetics; D011817:Rabbits; D014981:Xenopus; D029867:Xenopus Proteins
C498731:CLNS1A protein, Xenopus; D018118:Chloride Channels; D002712:Chlorides; D007473:Ion Channels; D018075:RNA, Complementary; D029867:Xenopus Proteins