Formation of ring-shaped structures on erythrocyte membranes after treatment with botulinolysin, a thiol-activated hemolysin from Clostridium botulinum.
Damage to erythrocyte membranes by botulinolysin (BLY) was studied by electron microscopy, which revealed ring-shaped structures with inner diameters and widths of approximately 32 and 6.7 nm, respectively. BLY bound to membranes at 0 degrees C, but subsequent treatment with glutaraldehyde prevented ring formation during further incubation at 37 degrees C. Zn2+ ions inhibited ring formation but not binding of BLY to membranes.
Sekiya
K
K
Department of Microbiology, School of Pharmaceutical Sciences, Kitasato University, Minato-ku, Tokyo 108-8641, Japan. sekiyak@platinum.pharm.kitasato-u.jp
Danbara
H
H
Futaesaku
Y
Y
Haque
A
A
Sugimoto
N
N
Matsuda
M
M
D001427:Bacterial Toxins / Q000494:pharmacology*; D003014:Clostridium botulinum; D004910:Erythrocyte Membrane / Q000187:drug effects* / Q000473:pathology; D006460:Hemolysin Proteins / Q000378:metabolism / Q000494:pharmacology*; D009930:Organic Chemicals; D011485:Protein Binding; D013696:Temperature; D015032:Zinc / Q000494:pharmacology
D001427:Bacterial Toxins; D006460:Hemolysin Proteins; D009930:Organic Chemicals; C112565:botulinolysin; D015032:Zinc