Glomerular thromboxane contributes to pressor response in deoxycorticosterone acetate-salt hypertension.
To assess the role of renal thromboxane in a salt sensitive pressor response in hypertension, urinary excretion of thromboxane and its release from isolated glomeruli and renal papillae were examined in deoxycorticosterone acetate treated rats with normal (0.6%, n = 12) and high (4%, n = 12) salt diets for 8 weeks. Mean blood pressure, measured directly by an implanted aortic catheter, was higher in the high salt diet group than in the normal salt diet group (146 +/- 2 vs 119 +/- 2 mmHg, P<0.01). Urinary excretion of thromboxane B2 and 6-keto-prostaglandin F1alpha in the high salt group were significantly higher than those in the normal salt diet group, but there was no difference in urinary excretion of prostaglandin E2 between the two groups. Release of thromboxane B2, 6-keto-prostaglandin F1alpha, and prostaglandin E2 from isolated glomeruli in the high salt diet group increased significantly by 104%, 55%, and 74%, respectively, compared with the normal salt diet group. Stepwise multiple linear regression analysis showed that significant contributory factors for mean blood pressure in deoxycorticosterone acetate treated rats were urinary excretion of sodium (F=14.187, P<0.01) and release of thromboxane B2 from isolated glomeruli (F=4.135, P<0.05). The unstandardized coefficient (R) calculated from the regression function using these two factors was 0.875 and R2 was 0.765. The manifest synthesis of thromboxane in renal glomeruli has an important role on salt sensitive pressor response in deoxycorticosterone acetate-salt hypertension of rats.
Kosugi
T
T
Department of Pediatrics, Nippon Telegraph and Telephone, Kanto Teishin Hospital, Shinagawa, Tokyo, Japan.
Ikeda
T
T
Gomi
T
T
Ushijima
T
T
Okada
J
J
D015121:6-Ketoprostaglandin F1 alpha / Q000652:urine; D000818:Animals; D001794:Blood Pressure / Q000187:drug effects / Q000502:physiology*; D003404:Creatinine / Q000097:blood; D003900:Desoxycorticosterone; D015232:Dinoprostone / Q000652:urine; D004195:Disease Models, Animal; D004305:Dose-Response Relationship, Drug; D005260:Female; D006339:Heart Rate / Q000187:drug effects / Q000502:physiology; D006973:Hypertension / Q000139:chemically induced / Q000503:physiopathology*; D007678:Kidney Glomerulus / Q000737:chemistry / Q000503:physiopathology; D007679:Kidney Medulla / Q000737:chemistry; D015999:Multivariate Analysis; D011188:Potassium / Q000097:blood; D011453:Prostaglandins / Q000652:urine; D051381:Rats; D017207:Rats, Sprague-Dawley; D012044:Regression Analysis; D012964:Sodium / Q000097:blood / Q000652:urine; D017673:Sodium Chloride, Dietary; D013929:Thromboxane B2 / Q000652:urine; D013931:Thromboxanes / Q000502:physiology*
D011453:Prostaglandins; D017673:Sodium Chloride, Dietary; D013931:Thromboxanes; D003900:Desoxycorticosterone; D013929:Thromboxane B2; D015121:6-Ketoprostaglandin F1 alpha; D012964:Sodium; D003404:Creatinine; D015232:Dinoprostone; D011188:Potassium