Analysis of the DNA damage induced by praziquantel in V-79 Chinese hamster fibroblasts and human blood cells using the single-cell gel electrophoresis assay.
The analysis of the genotoxicity of praziquantel, an effective antihelminthic widely used in countries where parasitic infections are still serious public health problems, has been extensively performed using diverse in vitro and in vivo assays and endpoints. However, results are not conclusive, since reports to date indicate either praziquantel is mutagenic, comutagenic, or even antimutagenic. In the present work, the clastogenic potential of praziquantel was investigated in V-79 Chinese hamster fibroblasts and human peripheral blood using a sensitive technique such as the single-cell electrophoresis assay. Results indicate that even though praziquantel induced DNA single-strand breaks both in V-79 cells and unstimulated human leukocytes, this effect was not translated into persistent DNA damage, since neither SCE nor HPRT mutations were induced. This suggests that the effect observed in the SCGE assay is an early event not closely related to praziquantel mutagenicity, because this DNA damage could be efficiently repaired.
Herrera
L A
LA
Instituto de Investigaciones Biomédicas, U.N.A.M., Ciudad Universitaria, Mexico City, Mexico.
Valverde
M
M
Ostrosky-Wegman
P
P
Speit
G
G
Rojas del Castillo
E
E
D000328:Adult; D000818:Animals; D000980:Antiplatyhelmintic Agents / Q000494:pharmacology*; D001773:Blood Cells / Q000737:chemistry / Q000187:drug effects*; D002460:Cell Line; D006224:Cricetinae; D003412:Cricetulus; D004247:DNA / Q000097:blood / Q000187:drug effects; D004249:DNA Damage; D004587:Electrophoresis, Agar Gel / Q000379:methods*; D005347:Fibroblasts / Q000737:chemistry / Q000187:drug effects; D006801:Humans; D011223:Praziquantel / Q000494:pharmacology*; D012854:Sister Chromatid Exchange / Q000187:drug effects
D000980:Antiplatyhelmintic Agents; D011223:Praziquantel; D004247:DNA