New positive/negative selectable markers for mammalian cells on the basis of Blasticidin deaminase-thymidine kinase fusions.
Two positive and negative selectable markers were created for use in mammalian cells. They are based on two genes for the resistance to Blasticidin S (BlaS) and on the thymidine kinase (Tk) gene of herpes simplex virus (HSV). The markers can be selected positively by their ability to induce BlaS resistance and negatively on the induced sensitivity towards gancyclovir (GANC). Both constructs are also expressed in Escherichia coli and transfer BlaS resistance to this organism as well, making these markers very suitable for the construction of shuttle vectors.
Karreman
C
C
GSF-National Research Center for Environment and Health, Institute of Clinical Molecular Biology and Tumour Genetics, Marchioninistrasse 25, 81377 Munich, Germany. karreman@gsf.de
D016475:3T3 Cells; D000619:Aminohydrolases / Q000235:genetics*; D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D001230:Aspergillus / Q000201:enzymology; D001409:Bacillus cereus / Q000201:enzymology; D004351:Drug Resistance; D004352:Drug Resistance, Microbial; D004926:Escherichia coli / Q000187:drug effects; D015774:Ganciclovir / Q000494:pharmacology; D005819:Genetic Markers; D005822:Genetic Vectors / Q000235:genetics; D051379:Mice; D009705:Nucleosides / Q000494:pharmacology; D011993:Recombinant Fusion Proteins; D012641:Selection, Genetic; D018139:Simplexvirus / Q000201:enzymology; D013937:Thymidine Kinase / Q000235:genetics*
D000900:Anti-Bacterial Agents; D005819:Genetic Markers; D009705:Nucleosides; D011993:Recombinant Fusion Proteins; C004500:blasticidin S; D013937:Thymidine Kinase; D000619:Aminohydrolases; C019795:blasticidin S deaminase; D015774:Ganciclovir