[Expression of basic fibroblast growth factor and its receptor in renal cell carcinoma].
Basic fibroblast growth factor (bFGF) is a pluripotent polypeptide which plays an important role in tumor progression and angiogensis. We determined the expression and localization patterns of bFGF and one of its receptor (FGFR-1) in normal renal as well as in renal cancers. The results were compared with clinicopathologic features. Using bFGF and FGFR-1 antibody, the repairing method of antigen with microwave oven heating and LSAB immunohistochemistry we used in 36 cases of paraffin-embedded renal cell carcinoma (RCC) and their paired normal renal tissues. The expression of bFGF and FGFR-1 was nearly consistent. The normal renal tissues and ECM of 29 cases of renal cancer tissues showed heterogenous immunoreactivities. Renal cancer cell cytoplasm of 12 primary tumors and 2 metastatic tumors, as in the cytoplasmic bFGF of cultured GRC-1 cells, were positively homogenous stained. The bFGF and FGFR-1 can be consistently expressed in normal renal and renal cancer tissues, reflecting that the expression and function of these substances were closely associated. The cytoplasmic bFGF expression of renal cancer was related to tumor stages, suggesting that b bFGF plays an important role in the progression of renal cell carcinoma.
Zhong
H
H
Institute of Urology, Beijing Medical University.
Deng
F
F
Kong
X
X
D018262:Adenocarcinoma, Clear Cell / Q000378:metabolism / Q000473:pathology; D000293:Adolescent; D000328:Adult; D000368:Aged; D000911:Antibodies, Monoclonal; D002292:Carcinoma, Renal Cell / Q000378:metabolism* / Q000473:pathology; D002648:Child; D005260:Female; D016222:Fibroblast Growth Factor 2 / Q000378:metabolism*; D006801:Humans; D007680:Kidney Neoplasms / Q000378:metabolism* / Q000473:pathology; D008297:Male; D008875:Middle Aged; D009367:Neoplasm Staging; D020794:Receptor Protein-Tyrosine Kinases; D051496:Receptor, Fibroblast Growth Factor, Type 1; D017468:Receptors, Fibroblast Growth Factor / Q000378:metabolism*
D000911:Antibodies, Monoclonal; D017468:Receptors, Fibroblast Growth Factor; D016222:Fibroblast Growth Factor 2; C496348:FGFR1 protein, human; D020794:Receptor Protein-Tyrosine Kinases; D051496:Receptor, Fibroblast Growth Factor, Type 1