Immunohistochemical localization of apolipoprotein B-100 (ApoB-100) and expression of glutathione peroxidase (GSH-PO) in canine atherosclerotic lesions.
We used immunohistochemistry to localize canine Apolipoprotein B-100 (CApoB-100) and glutathione peroxidase (GSH-PO) in canine atherosclerotic lesions. CApoB-100 was deposited in the tunica intima and cytoplasms of infiltrating macrophages in early atherosclerotic lesions. In advanced atherosclerotic lesions, the cystic space of the lesions contained a large amount of CApoB-100 immunoreactive material. Expression of GSH-PO was recognized in the foamy cytoplasm of macrophages and smooth muscle cells in the early and advanced atherosclerotic lesions. These results indicate that expression of GSH-PO is closely associated with the deposition of CApoB-100. In addition, they suggest that, as in human atheromas, low-density lipoprotein (LDL) is peroxidized and changed into modified LDL. Deposition of modified LDL (oxidized or acetylated) may be a critical step in the formation of canine atherosclerotic lesions.
Kagawa
Y
Y
Uchida
E
E
Yokota
H
H
Yamaguchi
M
M
Taniyama
H
H
D000818:Animals; D001011:Aorta / Q000378:metabolism / Q000473:pathology; D053299:Apolipoprotein B-100; D001055:Apolipoproteins B / Q000378:metabolism*; D001161:Arteriosclerosis / Q000378:metabolism / Q000473:pathology / Q000662:veterinary*; D003331:Coronary Vessels / Q000378:metabolism / Q000473:pathology; D004283:Dog Diseases / Q000378:metabolism* / Q000473:pathology; D004285:Dogs; D005487:Foam Cells / Q000378:metabolism / Q000473:pathology; D005979:Glutathione Peroxidase / Q000378:metabolism*; D007150:Immunohistochemistry; D008297:Male; D012077:Renal Artery / Q000378:metabolism / Q000473:pathology; D013154:Spleen / Q000098:blood supply / Q000473:pathology
D053299:Apolipoprotein B-100; D001055:Apolipoproteins B; D005979:Glutathione Peroxidase