Synthesis of guanosine 5'-triphosphate analogues and their effect on microtubule assembly.
More than 20 base-modified analogues of guanosine 5'-triphosphate including 2'-deoxyguanosine derivatives were synthesized and examined their effect on tubulin polymerization into microtubules (Mts). Among those, 2,6-diamino-8-oxopurineriboside 5'-triphosphate (1d), 2,6-diamino-2'-deoxypurineriboside 5'-tri phosphate (1b) and 8-bromoguanosine 5'-tri phosphate (1g) were shown to have remarkable effect to promote microtubule assembly.
Muraoka
M
M
Department of Chemical and Biological Sciences, Faculty of Science, Japan Women's University, Tokyo, Japan.
Fukuzawa
H
H
Nisita
A
A
Okano
K
K
Tsuchihara
T
T
Sakai
H
H
D006160:Guanosine Triphosphate / Q000031:analogs & derivatives* / Q000138:chemical synthesis / Q000737:chemistry / Q000494:pharmacology*; D007700:Kinetics; D008870:Microtubules / Q000187:drug effects* / Q000502:physiology; D015394:Molecular Structure; D013329:Structure-Activity Relationship; D014404:Tubulin / Q000187:drug effects / Q000378:metabolism*
D014404:Tubulin; D006160:Guanosine Triphosphate