title: Bacillus thuringiensis insecticidal proteins: molecular mode of action.
abstract: Growing interest in biorational pesticides has placed the Bacillus thuringiensis insecticidal crystal proteins at the forefront of pesticides for plant genetic engineering. The development of improvement pesticides, both in enhanced activity and broader host range, depends on an understanding of its mechanism of action. This review presents a complete overview of the bacterium and the group of insecticidal proteins known as Cry proteins or delta-endotoxins. The molecular mode of action is described in detail, including the mapping of receptor binding sites by site-directed mutagenesis, the known receptors, and the ion-channel activity of the toxins.
authors.affiliation: Department of Biochemistry, Ohio State University, Columbus 43210, USA.
authors.forename: F
authors.initials: F
authors.lastname: Rajamohan
authors.forename: M K
authors.initials: MK
authors.lastname: Lee
authors.forename: D H
authors.initials: DH
authors.lastname: Dean
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001413:Bacillus thuringiensis / Q000235:genetics; D000083722:Bacillus thuringiensis Toxins; D001426:Bacterial Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism / Q000494:pharmacology*; D001427:Bacterial Toxins; D001665:Binding Sites; D004731:Endotoxins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism / Q000494:pharmacology*; D006460:Hemolysin Proteins; D019476:Insect Proteins; D007313:Insecta / Q000187:drug effects / Q000378:metabolism; D007305:Insecticide Resistance; D007306:Insecticides / Q000737:chemistry / Q000378:metabolism / Q000494:pharmacology*; D007473:Ion Channels / Q000235:genetics / Q000378:metabolism; D008969:Molecular Sequence Data; D016297:Mutagenesis, Site-Directed; D011487:Protein Conformation; D011956:Receptors, Cell Surface / Q000378:metabolism
chemical_list: D000083722:Bacillus thuringiensis Toxins; D001426:Bacterial Proteins; D001427:Bacterial Toxins; C095416:Cry toxin receptors; D004731:Endotoxins; D006460:Hemolysin Proteins; D019476:Insect Proteins; D007306:Insecticides; D007473:Ion Channels; D011956:Receptors, Cell Surface; C030956:insecticidal crystal protein, Bacillus Thuringiensis