Investigation of anion-exchange and immunoaffinity particle-loaded membranes for the isolation of charged organic analytes from water.
Anion-exchange and immunoaffinity particle loaded membranes (PLMs) were investigated as a mechanism for the isolation of charged organic analytes from water. Kinetic properties determined theoretically included dynamic capacity, pressure drop (delta P), residence and diffusion times (Tr, Td), and total membrane porosity (epsilon T). These properties were confirmed through experimental evaluation, and the PLM method showed significant improvement over conventional solid-phase extraction (SPE) and ion-exchange formats. Recoveries of more than 90% were observed for a variety of test compounds at flow rates up to 70 mL/min (equipment-limited maximum flow rate). A fast-flow immunoaffinity column was developed using antibodies (Abs) attached to the PLMs. Reproducible recoveries (88% +/- 4%) were observed at flow rates up to 70 mL/min for the antibody (Ab)-loaded PLMs. Findings indicate increased selectivity over anion-exchange PLMs and conventional SPE or ion-exchange methods and rapid Ab-antigen binding rates given the excellent mass-transfer characteristics of the PLMs.
Dombrowski
T R
TR
U.S. Geological Survey, Lawrence, Kansas 66049, USA.
Wilson
G S
GS
Thurman
E M
EM
D002846:Chromatography, Affinity; D002852:Chromatography, Ion Exchange; D007120:Immunochemistry; D008567:Membranes, Artificial; D014874:Water Pollutants, Chemical / Q000032:analysis*; D014881:Water Supply / Q000032:analysis*
D008567:Membranes, Artificial; D014874:Water Pollutants, Chemical