Immunologic memory induced by a glycoconjugate vaccine in a murine adoptive lymphocyte transfer model.
We have developed an adoptive cell transfer model in mice to study the ability of a glycoprotein conjugate vaccine to induce immunologic memory for the polysaccharide moiety. We used type III capsular polysaccharide from the clinically relevant pathogen group B streptococci conjugated to tetanus toxoid (GBSIII-TT) as our model vaccine. GBS are a major cause of neonatal infections in humans, and type-specific antibodies to the capsular polysaccharide protect against invasive disease. Adoptive transfer of splenocytes from mice immunized with the GBSIII-TT conjugate vaccine conferred anti-polysaccharide immunologic memory to naive recipient mice. The transfer of memory occurred in a dose-dependent manner. The observed anamnestic immune response was characterized by (i) more rapid kinetics, (ii) isotype switching from immunoglobulin M (IgM) to IgG, and (iii) 10-fold-higher levels of type III-specific IgG antibody than for the primary response in animals with cells transferred from placebo-immunized mice. The adoptive cell transfer model described in this paper can be used for at least two purposes: (i) to evaluate conjugate vaccines with different physicochemical properties for their ability to induce immunologic memory and (ii) to study the cellular interactions required for an immune response to these molecules.
Guttormsen
H K
HK
Department of Medicine, Brigham and Women's Hospital, Harvard Medical School, Boston, Massachusetts 02115, USA. hilde-kari.guttormsen@channing.harvard.edu
Wetzler
L M
LM
Finberg
R W
RW
Kasper
D L
DL
D019264:Adoptive Transfer; D000818:Animals; D001428:Bacterial Vaccines / Q000276:immunology*; D006001:Glycoconjugates / Q000276:immunology*; D007074:Immunoglobulin G / Q000096:biosynthesis; D007075:Immunoglobulin M / Q000096:biosynthesis; D007156:Immunologic Memory; D008214:Lymphocytes / Q000276:immunology*; D051379:Mice; D008815:Mice, Inbred Strains; D013292:Streptococcus agalactiae / Q000276:immunology*; D013745:Tetanus Toxoid / Q000276:immunology
D001428:Bacterial Vaccines; D006001:Glycoconjugates; D007074:Immunoglobulin G; D007075:Immunoglobulin M; D013745:Tetanus Toxoid