[Cloning and identification of the Mycobacterium bovis BCG gene for signal peptide of antigen 85-B].
OBJECTIVE
To clone and identify the Mycobacterium bovis BCG gene for signal peptide of antigen 85-B.


METHODS
Using a polymerase chain reaction technique, the signal peptide sequence (position 94 to 211) of antigen 85-B was technique, which is one of the major protein secreted by Mycobacterium bovis BCG (Bacille-Calmette-Guerin). The gene of 117bp fragment was cloned into the SacI and EcoRI sites of pBluecript SK, and its sequence was determined by using T7DNA polymerase kit.


RESULTS
The sequence analysis showed that the sequence of 117bp signal peptide was not misincorporated and in 3' end understream of signal peptide the 12 multi cloning sites for foreign gene could be provided by the recombinant plasmid.


CONCLUSIONS
The signal sequence of BCG antigen 85-B was cloned into the pBluescript plasmid. The recombinant plasmid was called BCG-S01. According to the genetic information a secreted protein from M. bovis BCG could be utilized in making a useful vaccine vehicle to produce and secrete a vaccinal protein from M. bovis BCG living cells.
Zeng
X
X
Department of Medical Molecular Biology, Tongji Medical University, Wuhan.
Wang
G
G
Lu
Y
Y
D000942:Antigens, Bacterial / Q000235:genetics*; D001483:Base Sequence; D003001:Cloning, Molecular; D004269:DNA, Bacterial / Q000235:genetics; D008969:Molecular Sequence Data; D009163:Mycobacterium bovis / Q000276:immunology*; D021382:Protein Sorting Signals / Q000235:genetics*; D017421:Sequence Analysis
D000942:Antigens, Bacterial; D004269:DNA, Bacterial; D021382:Protein Sorting Signals; C065036:antigen 85, Mycobacterium bovis