Mucosa cell regeneration following intestinal ischemia/reperfusion injury in rats.
OBJECTIVE
To evaluate regeneration and proliferation of the mucosa cells following intestinal ischemia/reperfusion (I/R) injury.


METHODS
Sixty rats (200-250 g) were examined after intestinal I/R injury with immunohistochemical (BrdU) method and flow cytometric analysis.


RESULTS
Severe damage to the villi was evident at 2 hours reperfusion and the rate of cell proliferation was lower (P < 0.01). All the specimens has healed mucosa with re-epithelialized villi tips after 2 days regeneration, but the villi were scarce and tiny. After two and four days regeneration, the rate of cell proliferation was significantly higher than that of controls (P < 0.01).


CONCLUSIONS
Our study suggests that the intestinal regeneration following I/R injury in rats occurs early. The immunohistochemical method with BrdU-labeled technique is a rapid method for quantifying the rate of cellular DNA synthesis.
Zheng
S
S
Department of Pediatric Surgery, Children's Hospital, Shanghai Medical University, China.
Jin
B
B
Caty
M G
MG
Tjota
A
A
D000818:Animals; D002455:Cell Division; D004847:Epithelial Cells / Q000473:pathology; D007413:Intestinal Mucosa / Q000473:pathology* / Q000502:physiology; D007421:Intestine, Small / Q000098:blood supply*; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley; D012038:Regeneration; D015427:Reperfusion Injury / Q000473:pathology* / Q000503:physiopathology