title: [Molecular cloning and sequence analysis of glycoprotein D gene of herpes simplex virus 2 strain Sav and wild strain isolated].
abstract: OBJECTIVE
  To study the difference of structure and function of HSV-2 gD gene among the wild strain, G and Sav strain. So as to provide theoretical basis for future production of HSV-2 vaccine.
  
  
  METHODS
  The partial glycoprotein D gene sequence of HSV-2 strain Sav and wild strain isolated from recurred genital herpes simplex were amplified and cloned with PCR.
  
  
  RESULTS
  The homology comparison showed that the homology of DNA and amino acid were 99.2% and 99.1% respectively.
  
  
  CONCLUSIONS
  There are high homology of HSV-2 gD gene among the wild strain G and Sav strains, but some variations existed.
authors.affiliation: Guangzhou General Hospital of Guangzhou Military Command.
authors.forename: H
authors.initials: H
authors.lastname: Yang
authors.forename: M
authors.initials: M
authors.lastname: Li
authors.forename: Y
authors.initials: Y
authors.lastname: Jiang
mesh_terms: D000595:Amino Acid Sequence; D003001:Cloning, Molecular; D006558:Herpes Genitalis / Q000821:virology; D018258:Herpesvirus 2, Human / Q000145:classification / Q000235:genetics*; D006567:Herpesvirus 2, Saimiriine / Q000235:genetics*; D006801:Humans; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D017421:Sequence Analysis; D017385:Sequence Homology; D014759:Viral Envelope Proteins / Q000235:genetics*
chemical_list: D014759:Viral Envelope Proteins; C040570:glycoprotein D-herpes simplex virus type 2