title: Cellular localization of insulin-like growth factor II mRNA in the human fetus and the placenta: detection with a digoxigenin-labeled cRNA probe and immunocytochemistry.
abstract: IGF-II plays a major role in the regulation of human fetal growth and development. However, more extensive information on the cellular sites of IGF-II synthesis in the fetus would provide more insight into its role in fetal organogenesis. Thus we have determined the sites of IGF-II synthesis in 18-26-wk gestation human fetal tissues using in situ hybridization with a digoxigenin-labeled cRNA probe to localize IGF-II mRNA in fetal liver, kidney, adrenal gland, cerebral cortex, costal cartilage, skeletal muscle, and lung, and in placental tissue. In human fetal tissues it has to date been impossible to clearly assign IGF-II mRNA to epithelial cells of entodermal origin. Besides their already known localization in cell matrix and a variety of mesodermal cell types, strong IGF-II mRNA-positive signals were detected in epithelial cells in the liver (hepatocytes), bronchial and bronchiolar epithelium, undifferentiated renal tubular epithelium, mature glomerular epithelium, pelvic urothelium, and adrenal epithelial cells of the zona persistens. To identify the cellular location of immunoreactive IGF-II, we also performed immunocytochemical studies in tissues of the same fetuses. Every tissue studied except the cerebral cortex contained immunoreactive cells; however, immunostaining was generally weaker than in situ hybridization signals. Our data show that the distribution of IGF-II in human fetal tissue is much more widespread than hitherto thought. A digoxigenin-labeled detection system for IGF-II is more capable of detecting the cellular expression pattern of IGF-II than radioactive probes and is suitable for analysis of routinely prepared paraffin-embedded material.
authors.affiliation: Department of Neonatology and Intensive Care, University Children's Hospital Vienna, Austria.
authors.forename: R
authors.initials: R
authors.lastname: Birnbacher
authors.forename: G
authors.initials: G
authors.lastname: Amann
authors.forename: H
authors.initials: H
authors.lastname: Breitschopf
authors.forename: H
authors.initials: H
authors.lastname: Lassmann
authors.forename: G
authors.initials: G
authors.lastname: Suchanek
authors.forename: P
authors.initials: P
authors.lastname: Heinz-Erian
mesh_terms: D000028:Abortion, Induced; D004076:Digoxigenin; D005260:Female; D005333:Fetus / Q000166:cytology / Q000378:metabolism*; D005865:Gestational Age; D006801:Humans; D007150:Immunohistochemistry; D007335:Insulin-Like Growth Factor II / Q000096:biosynthesis*; D009928:Organ Specificity; D010920:Placenta / Q000378:metabolism*; D011247:Pregnancy; D015347:RNA Probes; D018075:RNA, Complementary; D012333:RNA, Messenger / Q000032:analysis*
chemical_list: D015347:RNA Probes; D018075:RNA, Complementary; D012333:RNA, Messenger; D007335:Insulin-Like Growth Factor II; D004076:Digoxigenin