Analyses of monoclonal antibodies reacting with porcine CD5: results from the Second International Swine CD Workshop.
Among the 57 monoclonal antibodies analyzed within the T-cell group, three mAbs fell within cluster T13 including the CD5a standard b53b7 (No. 174). The two new mAbs 1H6/8 (No. 058) and BB6-9G12 (No. 166) both precipitated 55 and 60 kDa proteins that were of similar molecular weights as the standard. Staining patterns on the various cell types were similar. Both new antibodies inhibited the binding of the CD5a reference mAbs b53b7 to peripheral lymphocytes. These mAbs, therefore both react with the CD5a epitope bringing the number of anti-porcine CD5 mAbs to eight, all of which appear to recognize the same epitope.
Pescovitz
M D
MD
Indiana University, Dept. of Surgery and Microbiology/Immunology, Indianapolis 46202, USA.
Book
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Aasted
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Dominguez
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Bullido
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Trebichavsky
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Novikov
B
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Valpotic
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Tomaskovic
M
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Nielsen
J
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Arn
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Sachs
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Lunney
J K
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Boyd
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Walker
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Lee
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Saalmüller
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D000818:Animals; D000911:Antibodies, Monoclonal / Q000032:analysis*; D000937:Antigen-Antibody Reactions; D001666:Binding Sites, Antibody; D001667:Binding, Competitive / Q000276:immunology; D018956:CD5 Antigens / Q000378:metabolism*; D005434:Flow Cytometry / Q000662:veterinary; D016131:Lymphocyte Subsets / Q000276:immunology; D011233:Precipitin Tests / Q000662:veterinary; D013552:Swine / Q000276:immunology*
D000911:Antibodies, Monoclonal; D018956:CD5 Antigens