Detection, purification and characterization of a protein that binds the (6-4) photoproduct-containing DNA in HeLa cells.
HeLa cell proteins that bind DNA containing the pyrimidine(6-4)pyrimidone photoproduct were detected by the electrophoretic mobility shift assay using synthetic oligonucleotide duplexes as probes. The major species was purified to near homogeneity, and the amino acid sequences of the proteolytic peptides revealed that it was the human damage-specific DNA-binding protein, which was reported previously. The substrate specificity of this protein was determined using damaged or modified DNA duplexes.
Fujiwara
Y
Y
Department of Bioorganic Chemistry, Biomolecular Engineering Research Institute, Osaka, Japan.
Masutani
C
C
Hanaoka
F
F
Iwai
S
S
D001483:Base Sequence; D002467:Cell Nucleus / Q000378:metabolism; D004249:DNA Damage; D004268:DNA-Binding Proteins / Q000302:isolation & purification / Q000378:metabolism*; D006367:HeLa Cells; D006801:Humans; D008969:Molecular Sequence Data; D009838:Oligodeoxyribonucleotides / Q000737:chemistry* / Q000378:metabolism*; D011740:Pyrimidine Dimers
D004268:DNA-Binding Proteins; D009838:Oligodeoxyribonucleotides; D011740:Pyrimidine Dimers; C058406:pyrimidine-pyrimidone dimer