title: Novel salivary gland specific binding elements located in the PSP proximal enhancer core.
abstract: The murine parotid secretory protein (PSP) gene is expressed selectively at high levels in parotid and sublingual salivary glands. Previously, the transcriptional activity of a PSP mini-gene, called Lama, was shown to be dependent on a 1.5 kb region located 3 kb upstream of the transcription start site. Here, functional studies in transgenic mice demonstrate that this proximal regulatory region has properties of a parotid and sublingual gland specific enhancer. Protein-binding experiments identify multiple sequence-specific binding complexes spanning the entire 1.5 kb enhancer region. Several sequence elements bound specifically by parotid and/or sublingual gland nuclear extracts, including consensus binding elements for previously described transcription factors as well as novel binding elements are located in the proximal enhancer region. A deletion analysis of the enhancer region in transgenic mice identified a core sequence of 700 bp. This region contains five elements bound specifically by nuclear proteins isolated from the PSP-expressing parotid and sublingual glands. Two of these elements, denoted parotid gland element I (PGE I) and sublingual gland element I (SLE I), are novel salivary gland specific binding elements, bound uniquely by parotid and sublingual gland nuclear extracts, respectively.
authors.affiliation: Department of Molecular and Structural Biology, Aarhus University, DK-8000 Aarhus C, Denmark.
authors.forename: P
authors.initials: P
authors.lastname: Svendsen
authors.forename: J
authors.initials: J
authors.lastname: Laursen
authors.forename: H
authors.initials: H
authors.lastname: Krogh-Pedersen
authors.forename: J P
authors.initials: JP
authors.lastname: Hjorth
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D010582:Bacteriophage lambda; D001483:Base Sequence; D004742:Enhancer Elements, Genetic; D005260:Female; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D008811:Mice, Inbred DBA; D008822:Mice, Transgenic; D008969:Molecular Sequence Data; D010306:Parotid Gland / Q000378:metabolism*; D012045:Regulatory Sequences, Nucleic Acid; D012471:Salivary Proteins and Peptides / Q000235:genetics*; D013361:Sublingual Gland / Q000378:metabolism*
chemical_list: C511597:Bpifa2 protein, mouse; D012471:Salivary Proteins and Peptides