title: Ovarian fecundity in patients with endometriosis can be estimated by the incidence of apoptotic bodies.
abstract: OBJECTIVE
  To investigate the incidence of apoptotic bodies in membrana granulosa in patients with endometriosis.
  
  
  METHODS
  Retrospective analysis.
  
  
  METHODS
  Yamagata University Hospital.
  
  
  METHODS
  Fifty-eight normoovulatory women with endometriosis and 45 patients with male factor infertility underwent ovulation induction for IVF-ET with GnRH analogues and gonadotropins.
  
  
  METHODS
  Patients underwent follicle aspiration after the administration of hCG.
  
  
  METHODS
  Recovered granulosa cells (GCs) were examined by fluorescence microscopy, and the incidence of apoptotic bodies was tabulated. These data and the other parameters associated with IVF results were analyzed on the basis of both the revised American Fertility Society (AFS) classification of endometriosis and the existence of chocolate cysts.
  
  
  RESULTS
  The incidence of apoptotic bodies in membrana granulosa of patients with endometriosis was significantly higher than that of the control (male factor infertility) group and increased as the stage of the revised AFS classification advanced. The incidence of apoptotic bodies in membrana granulosa was significantly higher in patients with chocolate cysts than in those without chocolate cysts. The patients with endometriosis had smaller numbers of developed follicles (> or = 15 mm), harvested oocytes, and mature oocytes than the male factor infertility patients. The existence of chocolate cysts corresponded with a reduced number of both harvested oocytes and mature oocytes.
  
  
  CONCLUSIONS
  Through the apoptosis of GCs, the existence of endometriosis may have negatively affected the follicle development and oocyte quality.
authors.affiliation: Department of Obstetrics and Gynecology, Yamagata University School of Medicine, Yamagata City, Japan.
authors.forename: K
authors.initials: K
authors.lastname: Nakahara
authors.forename: H
authors.initials: H
authors.lastname: Saito
authors.forename: T
authors.initials: T
authors.lastname: Saito
authors.forename: M
authors.initials: M
authors.lastname: Ito
authors.forename: N
authors.initials: N
authors.lastname: Ohta
authors.forename: T
authors.initials: T
authors.lastname: Takahashi
authors.forename: M
authors.initials: M
authors.lastname: Hiroi
mesh_terms: D000328:Adult; D017209:Apoptosis; D004715:Endometriosis / Q000473:pathology / Q000503:physiopathology*; D005260:Female; D005307:Fertilization in Vitro; D006107:Granulosa Cells / Q000473:pathology; D006801:Humans; D008297:Male; D006080:Ovarian Follicle / Q000503:physiopathology*; D011247:Pregnancy; D012189:Retrospective Studies