title: Localization of the Ca(2+)-binding S100A1 protein in slow and fast skeletal muscles of the rat.
abstract: The distribution of S100A1 in rat soleus and EDL muscles was studied immunocytochemically at the ultrastructural level using immunogold as marker. Antigens were localized mainly in myofibrils at all levels of the sarcomere. Immunogold particles along myofibrils were not uniformly distributed. The highest density of particles was found at Z-lines. An increase in particle density was observed in the middle of half A-bands in EDL and in the middle of half I-bands in the soleus. Antigen sites were also present at M-lines and at distinct locations of the sarcoplasmic reticulum.
authors.affiliation: Institute of Molecular Physiology and Genetics, Slovak Academy of Sciences, Bratislava, Slovakia.
authors.forename: B
authors.initials: B
authors.lastname: Maco
authors.forename: A
authors.initials: A
authors.lastname: Brezová
authors.forename: B W
authors.initials: BW
authors.lastname: Schäfer
authors.forename: B
authors.initials: B
authors.lastname: Uhrík
authors.forename: C W
authors.initials: CW
authors.lastname: Heizmann
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal; D002135:Calcium-Binding Proteins / Q000276:immunology / Q000378:metabolism*; D007150:Immunohistochemistry; D018656:Muscle Fibers, Fast-Twitch / Q000378:metabolism* / Q000648:ultrastructure; D018657:Muscle Fibers, Slow-Twitch / Q000378:metabolism* / Q000648:ultrastructure; D018482:Muscle, Skeletal / Q000378:metabolism* / Q000648:ultrastructure; D009210:Myofibrils / Q000378:metabolism; D051381:Rats; D009418:S100 Proteins; D012518:Sarcomeres / Q000378:metabolism; D012519:Sarcoplasmic Reticulum / Q000378:metabolism
chemical_list: D000911:Antibodies, Monoclonal; D002135:Calcium-Binding Proteins; D009418:S100 Proteins; C110294:S100A1 protein