In vivo expression of interleukin-1 receptors during various experimentally induced inflammatory conditions.
Systemically administered interleukin-1 (IL-1) has been shown to preferentially bind to IL-1 receptors (IL-1Rs) in inflammation. Using radiolabeled IL-1alpha and molecular methods to assess gene expression for these receptors, the in vivo behavior of these receptors was investigated in a number of experimental inflammatory conditions. The uptake of 125I-labeled IL-1alpha in inflammatory foci significantly correlated with the mRNA expression for the type I and type II IL-1Rs (P < .05). Type II IL-1R mRNA showed a greater increase in expression than type I IL-1R mRNA. In neutropenic mice, inflammatory lesions, which are devoid of granulocytes, significantly lower 125I-labeled IL-1alpha uptake (P < .001), and type II IL-1R mRNA expression (P < .005) was found. Thus, there is strong up-regulation of IL-1Rs at sites of focal inflammation. Of interest, this mainly involved the type II IL-1R on granulocytes, which is not involved in signal transduction.
van der Laken
C J
CJ
Department of Nuclear Medicine, University Hospital Nijmegen, The Netherlands. J.vanderLaken@Nugen.AZN.NL
Boerman
O C
OC
Oyen
W J
WJ
Laverman
P
P
van de Ven
M T
MT
Corstens
F H
FH
van der Meer
J W
JW
D000818:Animals; D002176:Candida albicans; D002177:Candidiasis / Q000276:immunology*; D004927:Escherichia coli Infections / Q000276:immunology*; D006801:Humans; D007249:Inflammation / Q000276:immunology*; D007375:Interleukin-1 / Q000378:metabolism* / Q000493:pharmacokinetics; D051379:Mice; D009503:Neutropenia; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000096:biosynthesis; D017472:Receptors, Interleukin-1 / Q000096:biosynthesis*; D011994:Recombinant Proteins / Q000378:metabolism / Q000493:pharmacokinetics; D013203:Staphylococcal Infections / Q000276:immunology*; D013211:Staphylococcus aureus; D014018:Tissue Distribution; D014158:Transcription, Genetic; D014425:Turpentine; D015054:Zymosan
D007375:Interleukin-1; D012333:RNA, Messenger; D017472:Receptors, Interleukin-1; D011994:Recombinant Proteins; D015054:Zymosan; D014425:Turpentine