Workshop studies with monoclonal antibodies identifying a novel porcine differentiation antigen, SWC9.
Two monoclonal antibodies (mAb) within cluster M4 of the myeloid section of the Second International Swine CD Workshop, C4 (No. 144) and PM18-7 (No. 192), showed reactivity with thymocytes and among cells of myelomonocytic origin with mature macrophages but not with monocytes and granulocytes. Both mAb recognize a protein showing two bands of 205 kDa and 130 kDa under both reducing and non-reducing conditions. Although epitope mapping with these mAb could not be performed, this cluster received the SWC9 designation.
Dominguez
J
J
Centro de Investigación en Sanidad Animal INIA, Madrid, Spain.
Ezquerra
A
A
Alonso
F
F
Bullido
R
R
McCullough
K
K
Summerfield
A
A
Bianchi
A
A
Zwart
R J
RJ
Kim
Y B
YB
Blecha
F
F
Eicher
S
S
Murtaugh
M P
MP
Pampusch
M
M
Burger
K
K
D000818:Animals; D000911:Antibodies, Monoclonal / Q000378:metabolism*; D015214:Antigens, Differentiation, Myelomonocytic / Q000032:analysis*; D007700:Kinetics; D008264:Macrophages / Q000276:immunology; D016676:Macrophages, Alveolar / Q000276:immunology; D009000:Monocytes / Q000276:immunology; D011233:Precipitin Tests; D013552:Swine / Q000276:immunology*; D013556:Swine, Miniature; D016176:T-Lymphocyte Subsets / Q000276:immunology
D000911:Antibodies, Monoclonal; D015214:Antigens, Differentiation, Myelomonocytic