title: Acetyl-CoA decarbonylase/synthase complex from Archaeoglobus fulgidus.
abstract: The acetyl-CoA decarbonylase/synthase (ACDS) multienzyme complex catalyzes the reversible cleavage and synthesis of acetyl-CoA in methanogens. This report of the enzyme complex in Archaeoglobus fulgidus demonstrates the existence of a functional ACDS complex in an organism that is not a methanogen. The A. fulgidus enzyme complex contained five subunits of 89, 72, 50, 49.5, and 18.5 kDa, and it catalyzed the overall synthesis of acetyl-CoA according to the following reaction: CO2 + 2 Fdred(Fe2+) + 2 H+ + CH3 - H4SPt + CoA <==> acetyl-CoA + H4SPt + 2 Fdox(Fe3+) + H2O where Fd is ferredoxin, and CH3-H4SPt and H4SPt denote N5-methyl-tetrahydrosarcinapterin and tetrahydrosarcinapterin, respectively.
authors.affiliation: Department of Biochemistry and Molecular Biology, Uniformed Services University of Health Sciences, Bethesda, MD 20814-4799, USA.
authors.forename: Y R
authors.initials: YR
authors.lastname: Dai
authors.forename: D W
authors.initials: DW
authors.lastname: Reed
authors.forename: J H
authors.initials: JH
authors.lastname: Millstein
authors.forename: P L
authors.initials: PL
authors.lastname: Hartzell
authors.forename: D A
authors.initials: DA
authors.lastname: Grahame
authors.forename: E
authors.initials: E
authors.lastname: DeMoll
mesh_terms: D000445:Aldehyde Oxidoreductases / Q000737:chemistry / Q000302:isolation & purification* / Q000378:metabolism; D000595:Amino Acid Sequence; D019843:Archaeal Proteins / Q000737:chemistry / Q000302:isolation & purification* / Q000378:metabolism; D019611:Archaeoglobus fulgidus / Q000201:enzymology*; D015153:Blotting, Western; D004591:Electrophoresis, Polyacrylamide Gel; D008969:Molecular Sequence Data; D009097:Multienzyme Complexes / Q000737:chemistry / Q000302:isolation & purification* / Q000378:metabolism
chemical_list: D019843:Archaeal Proteins; D009097:Multienzyme Complexes; D000445:Aldehyde Oxidoreductases; C019823:carbon monoxide dehydrogenase