title: Cell survival following bone-anterior cruciate ligament-bone allograft transplantation: DNA fingerprints, segregation, and collagen morphological analysis of multiple markers in the canine model.
abstract: Bone-anterior cruciate ligament-bone allograft transplantation has become recognized as a potential solution to reconstruction of the anterior cruciate ligament (ACL). The purpose of this study was to determine the time-dependent fibrocyte donor cell survival rate after cryopreserved bone-ACL-bone allograft transplantation. Additionally, bony incorporation of the pediculated bone plugs was examined. The ability to successfully transplant allogenous ACL fibrocytes and have them survive has not previously been documented. In this study, DNA fingerprints identified and documented the survival rate of the cellular DNA in transplanted ACL allografts for ACL re-construction in the knee joints of 10 skeletally mature dogs. At 4, 8, 26 and 52 weeks after ACL allograft transplantation, DNA probes, H & E, Giemsa, Goldner, PAS and polarized light staining was done to demonstrate the time-dependent changes in the allografts after transplantation. At 4 weeks host fibrocytes began to grow into the graft; however, histologically the cells could not be distinguished as to host or donor origin. After 4 weeks the DNA pattern reflected only the band pattern of the host. This reveals the early cellular infiltration activity of the host into the ACL allograft, also demonstrated in the light microscopy stainings. The survival rate of transplanted allogenous ACL fibrocytes had not been documented before this study. There is no evidence that ACL allograft cells survive in the intra-articular environment of the host's knee. Within 4 weeks ACL allografts became completely repopulated with host cells. The cells that migrate early into the ACL allografts are probably of synovial origin because they are present before revascularization and collagen reorganization occur. We conclude from this study that viable cells in transplanted ACL allografts did not survive longer than 4 weeks after intra-articular transplantation. Advances in molecular biology may offer new approaches to alter or stimulate fibrocyte population and function in the transplanted ACL allograft used for ACL reconstruction. New methods to maintain the viability of donor cells may be necessary to improve the biomechanical and histological properties of autografts or allografts for ACL reconstruction.
authors.affiliation: Department of Orthopaedic Surgery, Heinrich Heine University, Düsseldorf, Germany.
authors.forename: M J
authors.initials: MJ
authors.lastname: Goertzen
authors.forename: J
authors.initials: J
authors.lastname: Buitkamp
authors.forename: H
authors.initials: H
authors.lastname: Clahsen
authors.forename: M
authors.initials: M
authors.lastname: Möllmann
mesh_terms: D000818:Animals; D016118:Anterior Cruciate Ligament / Q000473:pathology / Q000637:transplantation*; D016025:Bone Transplantation / Q000473:pathology*; D002470:Cell Survival / Q000502:physiology*; D003094:Collagen / Q000648:ultrastructure*; D015925:Cryopreservation; D016172:DNA Fingerprinting; D004285:Dogs; D005347:Fibroblasts / Q000473:pathology; D006085:Graft Survival / Q000502:physiology; D008855:Microscopy, Electron, Scanning; D008859:Microscopy, Polarization; D014184:Transplantation, Homologous
chemical_list: D003094:Collagen