Analytical application of host-guest chemistry using in vitro selection.
A new bioassay system using oligodeoxyribonucleotides (DNAs) obtained by the in vitro selection method, instead of antibodies, is described. The DNAs which selectively bind to target molecules, folic acid, and thyroxine (T4), were selected. The DNAs were labeled with biotin or fluorescent molecules and the labeled DNA were used for detection of target molecules. The DNA strictly recognized the target molecules of which chemical structure is similar to each other.
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Graduate School of Material Science, NAIST, Ikoma, Japan.
D000906:Antibodies; D001483:Base Sequence; D001681:Biological Assay; D001710:Biotin; D004247:DNA / Q000737:chemistry; D005456:Fluorescent Dyes; D005492:Folic Acid / Q000032:analysis*; D008727:Methotrexate / Q000737:chemistry; D009838:Oligodeoxyribonucleotides / Q000737:chemistry*; D016133:Polymerase Chain Reaction; D013974:Thyroxine / Q000032:analysis*
D000906:Antibodies; D005456:Fluorescent Dyes; D009838:Oligodeoxyribonucleotides; D001710:Biotin; D004247:DNA; D005492:Folic Acid; D013974:Thyroxine; D008727:Methotrexate