Cooperative alkylation by duocarmycin A-distamycin A heterodimer.
Duo A (Duo) normally alkylates adenine N3 at the 3' end of A + T-rich sequence in DNA. The addition of another minor groove binder, dist A (Dist), dramatically modulate the site of DNA alkylation by Duo with great acceleration of the reaction rate. In order to examine the mode of alkylation, the kinetics of the reaction under various conditions were examined. Based on the simulation of experimental data, a new reaction pathway was proposed.
Ozeki
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Y
Department of Synthetic Chemistry and Biological Chemistry, Faculty of Engineering, Kyoto University, Japan.
Sugiyama
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H
Saito
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I
D000477:Alkylating Agents / Q000737:chemistry*; D000478:Alkylation; D000998:Antiviral Agents / Q000737:chemistry; D001483:Base Sequence; D004247:DNA / Q000737:chemistry*; D019281:Dimerization; D004214:Distamycins / Q000737:chemistry*; D000080890:Duocarmycins; D007211:Indoles; D009693:Nucleic Acid Hybridization; D009838:Oligodeoxyribonucleotides / Q000737:chemistry*; D011760:Pyrrolidinones / Q000737:chemistry
D000477:Alkylating Agents; D000998:Antiviral Agents; D004214:Distamycins; D000080890:Duocarmycins; D007211:Indoles; D009838:Oligodeoxyribonucleotides; D011760:Pyrrolidinones; C030000:stallimycin; D004247:DNA; C058289:duocarmycin A