title: A monoclonal antibody-based enzyme immunoassay for the measurement of native and glycated apolipoprotein B-containing particles.
abstract: We describe the development of sandwich enzyme-linked immunosorbent assays designed to measure native and glycated apolipoprotein B containing particles in plasma. The assays utilize monoclonal antibodies anti native or glycated apo B-LDL for coating and a polyclonal anti apoB-LDL-peroxidase conjugate as the detecting antibody. The method is specific, sensitive and precise. The intra assay coefficient of variation for the plasma native and glycated apolipoprotein B-containing particles was determine to be 7.8% and 7.5%, respectively. The method described can provide specific and reproducible determinations of apoB and glycated-apoB containing particles in plasma; it will be of great interest in the evaluation of atherosclerotic risk in dyslipoproteinemic states in diabetic and non-diabetic subjects.
authors.affiliation: Departamento de Bioquímica Clinicae Immunología, Facultad de Farmacia, Universidad de Concepción, Chile.
authors.forename: C
authors.initials: C
authors.lastname: Calvo
authors.forename: P
authors.initials: P
authors.lastname: Bustos
authors.forename: P
authors.initials: P
authors.lastname: Giraudo
authors.forename: N
authors.initials: N
authors.lastname: Ulloa
authors.forename: J
authors.initials: J
authors.lastname: Sepulveda
authors.forename: C
authors.initials: C
authors.lastname: Verdugo
authors.forename: L
authors.initials: L
authors.lastname: Toledo
mesh_terms: D000911:Antibodies, Monoclonal; D001055:Apolipoproteins B / Q000097:blood*; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D006031:Glycosylation; D006801:Humans; D015203:Reproducibility of Results
chemical_list: D000911:Antibodies, Monoclonal; D001055:Apolipoproteins B