[An experimental study on BMP gene expression in vivo].
The authors inserted the full length BMP-1 cDNA into Xbal-treated pRc/RSV plasmid via ligation, resulting in construction of pRc/RSV/BMP-1 eukaryotic expression vector. The validity of the reconstructed vector was confirmed by the restriction map. Then more reconstructed plasmids were extracted. According to the general principle of gene therapy, 100 micrograms of pRc/RSV/BMP-1 plasmid was transferred into a Balb/c mouse quadriceps by implantation under anaesthesia respectively. After 3 weeks, the BMP gene expression was detected in the mouse muscles by histoimmunochemistry.
Ma
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Dept of Orthopaedics, Third Teaching Hospital, Beijing Medical University.
Dang
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Ma
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D000818:Animals; D019485:Bone Morphogenetic Proteins / Q000096:biosynthesis / Q000235:genetics*; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D018482:Muscle, Skeletal / Q000378:metabolism; D011995:Recombination, Genetic
D019485:Bone Morphogenetic Proteins