Aminoacyl-tRNA synthesis in Archaea.
The mechanism of aminoacyl-tRNA synthesis differs substantially between Archaea, Bacteria and Eukarya. Sequencing of archaeal genomes has suggested that the asparaginyl-, cysteinyl-, glutaminyl- and lysyl-tRNA synthetases are absent from a number of organisms in this kingdom. The absence of the asparaginyl- and glutaminyl-tRNA synthetases is in agreement with the observation that Asn-tRNA and Gln-tRNA are synthesized by tRNA-dependent transamidation of Asp-tRNA and Glu-tRNA respectively in the archaeon Haloferax volcanii. Biochemical and genetic studies have now shown that while the cysteinyl- and lysyl-tRNA synthetases are present, the enzymes responsible for these activities are unique to Archaea.
Ibba
M
M
Department of Molecular Biophysics and Biochemistry, Yale University, New Haven, CT 06520-8114, USA.
Celic
I
I
Curnow
A
A
Kim
H
H
Pelaschier
J
J
Tumbula
D
D
Vothknecht
U
U
Woese
C
C
Söll
D
D
D000604:Amino Acyl-tRNA Synthetases / Q000235:genetics / Q000378:metabolism*; D019622:Haloferax volcanii / Q000235:genetics / Q000378:metabolism*; D008250:Lysine-tRNA Ligase / Q000235:genetics / Q000378:metabolism*; D012346:RNA, Transfer, Amino Acyl / Q000096:biosynthesis* / Q000378:metabolism
D012346:RNA, Transfer, Amino Acyl; D000604:Amino Acyl-tRNA Synthetases; C019975:cysteinyl-tRNA synthetase; D008250:Lysine-tRNA Ligase