The covalent coupling of Arg-Gly-Asp-containing peptides to liposomes: purification and biochemical function of the lipopeptide.
With the advent of liposomes as drug carriers, there arises a need for efficient targeted delivery in vivo. Proteins coupled to liposomes usually yield heterogeneous products that are ill-defined both chemically and in terms of spatial orientation. We now report on the disulfide linkage to the surface of intact liposomes of a peptide representing one-half of a ligand-receptor pair. An RGD-motif-containing peptide was coupled to the phospholipid PDP-DOPE of the liposomes by a thiol-disulfide exchange. The resulting lipopeptides were amenable to definition by TLC, HPLC, and MS and found to be in a functional orientation allowing biochemical interaction with their receptor, the integrin glycoprotein IIb-IIIa.
Gyongyossy-Issa
M I
MI
Canadian Red Cross Society Blood Services, University of British Columbia, 4750 Oak Street, Vancouver, British Columbia, Canada. missa@unixg.ubc.ca
Muller
W
W
Devine
D V
DV
D015060:1,2-Dipalmitoylphosphatidylcholine; D000595:Amino Acid Sequence; D002851:Chromatography, High Pressure Liquid; D004220:Disulfides; D007700:Kinetics; D008081:Liposomes / Q000737:chemistry*; D009842:Oligopeptides / Q000737:chemistry* / Q000302:isolation & purification; D010714:Phosphatidylethanolamines / Q000737:chemistry; D011485:Protein Binding; D011725:Pyridines / Q000737:chemistry; D013329:Structure-Activity Relationship; D013438:Sulfhydryl Compounds
C112318:1,2-dioleoyl-sn-glycero-3--phosphatidylethanolamine-N-(3-(2-pyridyldithio)propionate); D004220:Disulfides; D008081:Liposomes; D009842:Oligopeptides; D010714:Phosphatidylethanolamines; D011725:Pyridines; D013438:Sulfhydryl Compounds; D015060:1,2-Dipalmitoylphosphatidylcholine; C047981:arginyl-glycyl-aspartic acid