Treatment of equine oocytes with A23187 after intracytoplasmic sperm injection.
In vitro matured horse oocytes with a first polar body (n = 68) were each injected with a single spermatozoon and divided into 2 groups: Group 1 oocytes were treated with 10 microM calcium ionophore A23187 for 5 min while Group 2 oocytes received no activation treatment. After culture in vitro for 2 days, significantly more oocytes treated with A23187 (5/24, 21%) cleaved than oocytes without activation treatment (2/44, 5%, P<0.05). All 7 cleaved zygotes from both treatment groups were transferred to recipient mares but no pregnancies resulted.
Kato
H
H
Animal Reproduction and Biotechnology Laboratory, Colorado State University, Fort Collins 80523, USA.
Seidel
G E
GE
Squires
E L
EL
Wilson
J M
JM
D000818:Animals; D000001:Calcimycin / Q000494:pharmacology*; D004624:Embryo Transfer / Q000662:veterinary; D005260:Female; D005307:Fertilization in Vitro / Q000379:methods / Q000662:veterinary*; D006736:Horses / Q000502:physiology*; D007476:Ionophores / Q000494:pharmacology*; D008297:Male; D008845:Microinjections; D009865:Oocytes / Q000187:drug effects* / Q000254:growth & development; D013094:Spermatozoa / Q000166:cytology
D007476:Ionophores; D000001:Calcimycin