Mechanisms of the genotoxic effects associated with 5-formyluracil: effect of exogenous 5'-formyl-2'-deoxyuridine.
E. coli HB101 harboring plasmid pUC19 was grown in the presence of 5-formyl-2'-deoxyuridine (fdU) to evaluate the genotoxic and cytotoxic potentials associated with this DNA lesion. Cell growth was inhibited by fdU in a concentration-dependent manner, but increased mutation was not observed in the lacZ(alpha) gene of pUC19. The lack of the mutagenic effect was attributed to poor utilization of fdU as a substrate by thymidine kinase, which converts exogenous thymidine analogs to the corresponding 5'-monophosphates in the salvage pathway.
Ide
H
H
Graduate Department of Gene Science, Faculty of Science, Hiroshima University, Higashi-Hiroshima, Japan.
Terato
H
H
Masaoka
A
A
Nagasawa
N
N
Ohyama
Y
Y
D002455:Cell Division / Q000187:drug effects; D003857:Deoxyuridine / Q000031:analogs & derivatives* / Q000494:pharmacology; D004926:Escherichia coli / Q000187:drug effects* / Q000235:genetics / Q000254:growth & development; D016296:Mutagenesis; D009152:Mutagenicity Tests; D009153:Mutagens / Q000494:pharmacology*; D010957:Plasmids / Q000187:drug effects; D013937:Thymidine Kinase / Q000378:metabolism; D014498:Uracil / Q000031:analogs & derivatives* / Q000494:pharmacology
D009153:Mutagens; C007777:5-formyluracil; C061519:5-formyl-2'-deoxyuridine; D014498:Uracil; D013937:Thymidine Kinase; D003857:Deoxyuridine