title: The Platelet Function Analyzer (PFA-100): a novel in-vitro system for evaluation of primary haemostasis in children.
abstract: The PFA-100 system provides an in-vitro method of assessing primary platelet-related haemostasis by measuring the time (the closure time, or CT) taken for a platelet plug to occlude a microscopic aperture cut into a membrane coated with collagen and either epinephrine or ADP. We used the system to establish normal ranges for CTs in healthy children, adults and neonates. Mean CTs of healthy children were independent of the needle gauge used (21G or 23CG) for blood sampling; they were very similar to the mean CTs of healthy adults, but longer than mean CTs of healthy neonates. Although children with haemophilia had normal CTs, the PFA-100 system was found to be potentially useful in screening for von Willebrand disease in children.
authors.affiliation: Division of Haematology/Oncology, The Hospital for Sick Children, Toronto, ON, Canada.
authors.forename: M D
authors.initials: MD
authors.lastname: Carcao
authors.forename: V S
authors.initials: VS
authors.lastname: Blanchette
authors.forename: J A
authors.initials: JA
authors.lastname: Dean
authors.forename: L
authors.initials: L
authors.lastname: He
authors.forename: M A
authors.initials: MA
authors.lastname: Kern
authors.forename: A M
authors.initials: AM
authors.lastname: Stain
authors.forename: C R
authors.initials: CR
authors.lastname: Sparling
authors.forename: D
authors.initials: D
authors.lastname: Stephens
authors.forename: G
authors.initials: G
authors.lastname: Ryan
authors.forename: J
authors.initials: J
authors.lastname: Freedman
authors.forename: M L
authors.initials: ML
authors.lastname: Rand
mesh_terms: D000293:Adolescent; D000328:Adult; D001777:Blood Coagulation / Q000502:physiology; D001780:Blood Coagulation Tests / Q000379:methods*; D001792:Blood Platelets / Q000502:physiology*; D002648:Child; D002675:Child, Preschool; D006467:Hemophilia A / Q000175:diagnosis / Q000503:physiopathology; D006487:Hemostasis; D006801:Humans; D066298:In Vitro Techniques; D007223:Infant; D007231:Infant, Newborn; D008875:Middle Aged; D012016:Reference Values; D014842:von Willebrand Diseases / Q000175:diagnosis / Q000503:physiopathology