Solid phase synthesis of U1 RNA oligomers containing a TMG cap structure.
We report the solid-phase synthesis of a 5'-terminal part of U1 RNA. In this approach, a new method for pyro- and tri-phosphate bond formation on solid supports was studied. Since 2,2,7-trimethyl guanosine (TMG) in the cap structure was unstable under basic conditions, a RNA oligomer was synthesized by using a linker having the P-N bond, which can be cleaved from the solid support by treatment with 80% acetic acid.
Kadokura
M
M
Department of Life Science, Faculty of Bioscience and Biotechnology, Tokyo Institute of Technology, Yokohama, Japan.
Ushioda
M
M
Wada
T
T
Sekine
M
M
D006151:Guanosine / Q000031:analogs & derivatives; D007202:Indicators and Reagents; D009843:Oligoribonucleotides / Q000138:chemical synthesis* / Q000737:chemistry; D012315:RNA Caps / Q000138:chemical synthesis* / Q000737:chemistry; D012342:RNA, Small Nuclear / Q000138:chemical synthesis* / Q000737:chemistry
D007202:Indicators and Reagents; D009843:Oligoribonucleotides; D012315:RNA Caps; D012342:RNA, Small Nuclear; D006151:Guanosine; C017778:N(2),N(2),7-trimethylguanosine