[A simple method of studying oxidation resistance of heparin-precipitated blood beta lipoproteins].
Decrease of beta-lipoprotein resistance to copper-dependent oxidation is an important diagnostic criterion of risk of atherosclerosis. We developed a simple method for assessing resistance of heparin-precipitated beta-lipoproteins to oxidation induced by 50 microM copper ions. A strong positive correlation between the oxidation of precipitated and ultracentrifuged beta-lipoproteins in the presence of 50 and 10 microM copper ions, respectively, was revealed. In coronary patients oxidation resistance of precipitated beta-lipoproteins was decreased in comparison with that in healthy donors. The method is simple, informative, and easily available for clinical biochemical laboratories.
Ragino
Iu I
IuI
Dushkin
M I
MI
D000328:Adult; D000925:Anticoagulants / Q000494:pharmacology; D003300:Copper / Q000494:pharmacology; D003327:Coronary Disease / Q000097:blood*; D003627:Data Interpretation, Statistical; D006493:Heparin / Q000494:pharmacology; D006801:Humans; D015227:Lipid Peroxidation / Q000187:drug effects; D008077:Lipoproteins, LDL / Q000097:blood* / Q000378:metabolism; D008297:Male; D008875:Middle Aged; D014461:Ultracentrifugation
D000925:Anticoagulants; D008077:Lipoproteins, LDL; D003300:Copper; D006493:Heparin
Prostoĭ metod issledovaniia rezistentnosti k okisleniiu geparinosazhdennykh beta-lipoproteinov syvorotki krovi.