Establishment of a mouse clonal early proximal tubule cell line and outer medullary collecting duct cells expressing P2 purinoceptors.
The purpose of this study was to establish tubule cells expressing P2 purinoceptors. Use of the polymerase chain reaction coupled with reverse transcription showed that mouse clonal early proximal tubule (S1) cell line (NF-5) and outer medullary collecting tubule (OMCT) cells expressed mRNA for P2X4, P2Y1 and P2Y2 purinoceptors. ATP and its analogues induced a dose-dependent increase in cytosolic free calcium concentration ([Ca2+]i) in both NF-5 and OMCT cells. ATP enhanced [3H]-thymidine uptake by both NF-5 and OMCT cells in a dose-dependent manner. In conclusion, NF-5 and OMCT cells can be used in the further analysis of the function and regulation of activity and expression of P2 purinoceptors in the renal tubule.
Takeda
M
M
Department of Pharmacology and Toxicology, Kyorin University School of Medicine, Tokyo, Japan.
Kobayashi
M
M
Endou
H
H
D000255:Adenosine Triphosphate / Q000031:analogs & derivatives / Q000494:pharmacology; D000818:Animals; D002118:Calcium / Q000378:metabolism; D002460:Cell Line; D007679:Kidney Medulla / Q000166:cytology / Q000378:metabolism / Q000648:ultrastructure*; D007685:Kidney Tubules, Collecting / Q000166:cytology / Q000378:metabolism / Q000648:ultrastructure*; D007687:Kidney Tubules, Proximal / Q000166:cytology / Q000378:metabolism / Q000648:ultrastructure*; D051379:Mice; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D018048:Receptors, Purinergic P2 / Q000096:biosynthesis*; D013936:Thymidine / Q000493:pharmacokinetics; D014158:Transcription, Genetic; D014316:Tritium
D012333:RNA, Messenger; D018048:Receptors, Purinergic P2; D014316:Tritium; D000255:Adenosine Triphosphate; D002118:Calcium; D013936:Thymidine