Immunohistochemical detection of ribosomal transcription factor UBF: diagnostic value in malignant specimens.
The nucleolar organizer regions (NORs) of human chromosome can be identified in interphase and mitotic cells by localization of some intrinsic components such as the associated enzyme RNA polymerase I. A new sensitive staining method for NORs is described using a specific antibody to the ribosomal transcription factor UBF. By indirect immunofluorescence and enzyme-labelling methods, NORs stained in benign and malignant cells from a variety of tissues with monospecific anti-UBF serum showed significant morphological differences which correlated well with histopathological evaluation. The number of NORs per cell in malignant preparations increased significantly. Furthermore, the staining of a NOR protein component such as UBF appears to be as sensitive as the silver-staining technique (AgNOR) and might be a better alternative for detecting ribosomal activity in malignant tissues.
Torres-Montaner
A
A
Servicio de Anatomia Patológica, Hospital Clínico, Cádiz, Spain.
Bolivar
J
J
Ortiz
M
M
Valdivia
M M
MM
D014408:Biomarkers, Tumor / Q000378:metabolism*; D004268:DNA-Binding Proteins / Q000276:immunology / Q000378:metabolism*; D005455:Fluorescent Antibody Technique; D006801:Humans; D007106:Immune Sera; D007124:Immunoenzyme Techniques; D008223:Lymphoma / Q000175:diagnosis / Q000378:metabolism; D009363:Neoplasm Proteins / Q000378:metabolism*; D009369:Neoplasms / Q000175:diagnosis* / Q000378:metabolism; D009697:Nucleolus Organizer Region / Q000378:metabolism*; D035463:Pol1 Transcription Initiation Complex Proteins; D016622:Silver Staining; D014157:Transcription Factors / Q000276:immunology / Q000378:metabolism*
D014408:Biomarkers, Tumor; D004268:DNA-Binding Proteins; D007106:Immune Sera; D009363:Neoplasm Proteins; D035463:Pol1 Transcription Initiation Complex Proteins; D014157:Transcription Factors; C063844:transcription factor UBF