[The effect of implantation of reconstituted bone xenograft on the production of IL-2].
The present study is designed to investigate any possible effect of implantation of reconstituted bone xenograft (RBX) on the production of IL-2 by T-lymphocytes in female BALB/C mice model. IL-2 levels were elevated in all of the operated groups on the second day, suggesting a stress reaction to trauma caused by operative intervention rather than an immune response to RBX or bBMP. IL-2 production declined significantly on the 7th (P < 0.05) and 14th days (P < 0.01) in RBX and bBMP groups, an evidence of inhibiting effect of RBX on the T-lymphocyte function leading to lowered secretion of IL-2 by spleen T-cells in BALB/C mice. There was no significant difference in lowering of IL-2 secretion between the RBX and bBMP groups as seen when either was compared with the bCB group, suggesting an inhibiting action induced by BMP. The results demonstrated that RBX implantation was not associated with appreciable rejection in that activation, and proliferation of T-cells were inhibited and the secretion of cytokines was lowered resulting in suppressed cellular immunity. We are further convinced that RBX as an immuno-regulating cytokine possesses strong osteo-inductive potential without evoking rejection.
Zhao
C
C
Department of Orthopedics, 202 Army Hospital, Shenyang.
Hu
Y
Y
D000818:Animals; D019485:Bone Morphogenetic Proteins / Q000494:pharmacology*; D016025:Bone Transplantation; D005260:Female; D007376:Interleukin-2 / Q000378:metabolism*; D051379:Mice; D008807:Mice, Inbred BALB C; D011897:Random Allocation; D013601:T-Lymphocytes / Q000378:metabolism; D014183:Transplantation, Heterologous
D019485:Bone Morphogenetic Proteins; D007376:Interleukin-2