title: Construction and characterization of an isogenic slt-ii deletion mutant of enterohemorrhagic Escherichia coli.
abstract: Enterohemorrhagic Escherichia coli (EHEC) produces Shiga-like toxins (SLT), potent protein synthesis inhibitors. To further dissect the role of SLT-II in the course of disease, we have constructed E. coli TUV86-2, an isogenic SLT-II-negative mutant of EHEC strain 86-24. The slt-ii gene was inactivated by suicide vector mutagenesis. We also isolated derivatives of strain 86-24 that were cured of the phage carrying the toxin genes.
authors.affiliation: Division of Infectious Diseases, Tufts University School of Veterinary Medicine, North Grafton, Massachusetts 01536, USA.
authors.forename: F
authors.initials: F
authors.lastname: Gunzer
authors.forename: U
authors.initials: U
authors.lastname: Bohn
authors.forename: S
authors.initials: S
authors.lastname: Fuchs
authors.forename: I
authors.initials: I
authors.lastname: Mühldorfer
authors.forename: J
authors.initials: J
authors.lastname: Hacker
authors.forename: S
authors.initials: S
authors.lastname: Tzipori
authors.forename: A
authors.initials: A
authors.lastname: Donohue-Rolfe
mesh_terms: D000818:Animals; D001427:Bacterial Toxins / Q000235:genetics / Q000633:toxicity*; D004768:Enterotoxins / Q000633:toxicity*; D004926:Escherichia coli / Q000235:genetics / Q000472:pathogenicity*; D051379:Mice; D008807:Mice, Inbred BALB C; D009154:Mutation; D022641:Shiga Toxin 2
chemical_list: D001427:Bacterial Toxins; D004768:Enterotoxins; D022641:Shiga Toxin 2