The analysis of mRNA expression of cytokines from skin lesions in Churg-Strauss syndrome.
We report our recent clinical experience with a patient suffering from Churg-Strauss syndrome and the results of our investigation into the mRNA expression of cytokines in the patient's lesions as well as in the frozen sections from a previous patient. In both cases, blood IgG was at a high level. Cytokine mRNA expression differed according to the degree of cellular infiltration. In the presence of marked infiltration, counteracting Th1 and Th2 cytokines were simultaneously detected; the former included IL-12 and IFN-gamma, and the latter, IL-6 and IL-10. The concurrence of both types of cytokine could be attributed to several factors. For example, IL-6 is involved through some mechanism in the formation of immune complexes by IgG, and IL-12 and IFN-gamma appeared to participate in the development of granuloma. These suppositions support the suggested immunological etiology of the disease. It is also inferred that the dominance of one of the two types of cytokines depends on the clinical phase of the disease.
Fujioka
A
A
Department of Dermatology, Tokyo Metropolitan Hospital of Gerontology, Japan.
Yamamoto
T
T
Takasu
H
H
Kawano
K
K
Masuzawa
M
M
Katsuoka
K
K
Jinno
S
S
D015415:Biomarkers / Q000032:analysis; D001707:Biopsy, Needle; D015267:Churg-Strauss Syndrome / Q000175:diagnosis / Q000473:pathology* / Q000503:physiopathology; D016207:Cytokines / Q000235:genetics*; D006801:Humans; D007074:Immunoglobulin G / Q000032:analysis; D008297:Male; D008875:Middle Aged; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000032:analysis*; D012680:Sensitivity and Specificity; D012867:Skin / Q000473:pathology*
D015415:Biomarkers; D016207:Cytokines; D007074:Immunoglobulin G; D012333:RNA, Messenger