title: New compounds with DNA strand-scission activity from the combined leaf and stem of Uvaria hamiltonii.
abstract: Two flavanones, hamiltones A (1) and B (2), an aurone, hamiltrone (3), a chalcone, hamilcone (4), and a tetrahydroxanthene, hamilxanthene (5), were isolated from Uvaria hamiltonii extracts guided initially by fractionation based on DNA strand-scission and/or 9KB cytotoxicity assays. Compounds 2-5 have not been reported previously, while 1 is new as a natural product. Structural assignments were made based on extensive spectroscopic measurements. Compounds 1-3 were inactive in the 9KB cytotoxicity assay, with compounds 4 and 5 having weak activity. In the DNA strand-scission assay, 3 was the most active compound found in the DNA strand-scission assay, being 10 times more potent than 1 or 2. Compound 4 was only weakly active, and 5 was inactive.
authors.affiliation: Chemistry and Life Sciences Group, Research Triangle Institute, North Carolina 22709, USA.
authors.forename: L
authors.initials: L
authors.lastname: Huang
authors.forename: M E
authors.initials: ME
authors.lastname: Wall
authors.forename: M C
authors.initials: MC
authors.lastname: Wani
authors.forename: H
authors.initials: H
authors.lastname: Navarro
authors.forename: T
authors.initials: T
authors.lastname: Santisuk
authors.forename: V
authors.initials: V
authors.lastname: Reutrakul
authors.forename: E K
authors.initials: EK
authors.lastname: Seo
authors.forename: N R
authors.initials: NR
authors.lastname: Farnsworth
authors.forename: A D
authors.initials: AD
authors.lastname: Kinghorn
mesh_terms: D000972:Antineoplastic Agents, Phytogenic / Q000302:isolation & purification* / Q000494:pharmacology; D004247:DNA / Q000187:drug effects*; D004249:DNA Damage; D004354:Drug Screening Assays, Antitumor; D044950:Flavanones; D005419:Flavonoids / Q000302:isolation & purification* / Q000494:pharmacology; D006801:Humans; D008968:Molecular Conformation; D018515:Plant Leaves / Q000737:chemistry*; D010946:Plants, Medicinal / Q000737:chemistry*; D013056:Spectrophotometry, Ultraviolet; D014407:Tumor Cells, Cultured; D014966:Xanthenes / Q000302:isolation & purification* / Q000494:pharmacology
chemical_list: D000972:Antineoplastic Agents, Phytogenic; D044950:Flavanones; D005419:Flavonoids; D014966:Xanthenes; C112364:hamiltone A; C112365:hamiltone B; C112366:hamilxanthene; D004247:DNA