Hydrogen ion equilibria of Cajanus cajan lectin.
Hydrogen ion titration of an affinity-purified mannose/glucose-specific lectin from Cajanus cajan pulse was carried out at 30 degrees C and ionic strength of 0.15 by a discontinuous method. The titration was reversible in the pH range 2-12.0. The numbers of different ionizable groups per 39,000 g of the lectin were 43 carboxyl groups (pKint = 3.93), 10 imidazole groups, 21 epsilon-amino groups, 12.8 phenoxyl groups (pKint = 10.0), and 5 guanidyl groups. Only seven tyrosine residues of the lectin were dissociated under native conditions. The remaining six tyrosines became available for titration upon denaturation of the lectin in 9 M urea.
Salahuddin
A
A
Department of Biochemistry, J.N.M.C., and Institute of Biotechnology, A.M.U., Aligarth (U.P.), India.
Khan
R H
RH
D007887:Fabaceae / Q000737:chemistry*; D006863:Hydrogen-Ion Concentration; D037102:Lectins / Q000737:chemistry*; D009994:Osmolar Concentration; D037121:Plant Lectins; D010946:Plants, Medicinal; D011489:Protein Denaturation; D011522:Protons; D013696:Temperature; D017186:Titrimetry; D014443:Tyrosine / Q000737:chemistry*
D037102:Lectins; D037121:Plant Lectins; D011522:Protons; D014443:Tyrosine