title: BetaAPP gamma-secretase and SREBP site 2 protease are two different enzymes.
abstract: The cleavage process of sterol regulatory element binding proteins (SREBPs) shares several characteristics with that of beta-amyloid precursor protein (betaAPP). We studied the secretion of amyloid beta protein (Abeta) from mutant M19 Chinese hamster ovary (CHO) cells that are defective in the site 2 cleavage of SREBPs. The levels as well as the C-terminal properties of Abeta secreted from M19 cells were similar to those from wild-type CHO cells, demonstrating that gamma-cleavage of betaAPP occurs normally in M19 cells. The present data indicated that gamma-secretase of betaAPP and SREBP site 2 protease are distinct enzymes.
authors.affiliation: Department of Neuropathology and Neuroscience, Graduate School of Pharmaceutical Sciences, University of Tokyo, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Tomita
authors.forename: T Y
authors.initials: TY
authors.lastname: Chang
authors.forename: T
authors.initials: T
authors.lastname: Kodama
authors.forename: T
authors.initials: T
authors.lastname: Iwatsubo
mesh_terms: D053829:Amyloid Precursor Protein Secretases; D016564:Amyloid beta-Protein Precursor / Q000737:chemistry / Q000378:metabolism*; D000818:Animals; D016282:Aspartic Acid Endopeptidases; D015153:Blotting, Western; D022762:CCAAT-Enhancer-Binding Proteins; D016466:CHO Cells; D002460:Cell Line; D006224:Cricetinae; D004268:DNA-Binding Proteins / Q000378:metabolism*; D010450:Endopeptidases / Q000737:chemistry / Q000378:metabolism*; D004797:Enzyme-Linked Immunosorbent Assay; D051379:Mice; D008970:Molecular Weight; D009687:Nuclear Proteins / Q000378:metabolism*; D051780:Sterol Regulatory Element Binding Protein 1; D014157:Transcription Factors / Q000378:metabolism*
chemical_list: D016564:Amyloid beta-Protein Precursor; D022762:CCAAT-Enhancer-Binding Proteins; D004268:DNA-Binding Proteins; D009687:Nuclear Proteins; C497388:Srebf1 protein, mouse; D051780:Sterol Regulatory Element Binding Protein 1; D014157:Transcription Factors; D053829:Amyloid Precursor Protein Secretases; D010450:Endopeptidases; D016282:Aspartic Acid Endopeptidases; C509282:Bace1 protein, mouse; C112686:SREBP site 2 protease