Random amplified polymorphic DNA observed in Eucalyptus by PCR study with random primers.
A total DNA extracted from Eucalyptus globlus, E. citriodora, or a subspecies of E. gunnii (Maruha Yu-kari) being used as an template, Polymerase Chain Reaction (PCR) was performed with 40 kinds of commercially available random primers (Operon, OPA-01-20 and OPB-01-20). After electrophoresis of PCR-products on agarose gel, we analyzed the band pattern formed by DNA-fragments with 0.5-3.0 kbp as molecular weight. As a remuneration for the examination in which forty 10-mer arbitrary oligonucleotides were tried to be used as primers in PCR for each species, we found that 9 primers (for E. globlus) leads polymorphism, 6 primers for E. citriodora, and 6 primers for Maruha Yu-kari.
Izumi
M
M
Biochemistry Section, Iwakuni Research Laboratory of Technology, Nippon Paper Industries Co. Ltd, Yamaguchi, Japan.
Kojima
E
E
Matsuda
M
M
Shimizu
T
T
Murakami
A
A
Tanabe
T
T
Murakami
K
K
D001483:Base Sequence; D017931:DNA Primers; D018744:DNA, Plant / Q000737:chemistry* / Q000235:genetics; D009876:Operon; D011110:Polymorphism, Genetic; D019105:Random Amplified Polymorphic DNA Technique; D012639:Seeds; D013045:Species Specificity; D014197:Trees / Q000145:classification / Q000235:genetics*
D017931:DNA Primers; D018744:DNA, Plant