title: [Development of an enzyme assay for detection of hepatitis B virus core IGM antibodies].
abstract: Detection of IgM anti-core (anti-HBcAg) antibodies of Hepatitis B Virus (HBV) is an useful marker for hepatitis B virus (HBV) acute infection. The aim of this study was to perform an immunodiagnostic assay for the detection of IgM anti-HBcAg antibodies. Hepatitis B core antigen (HBcAg) was produced by a recombinant clone of Escherichia coli and used for the development of the immunoassay. An IgM capture enzyme immunoassay (EIA) was selected for the detection of IgM anti-HBcAg antibodies. A total of 110 human plasma or sera were tested by the capture EIA and a commercial assay. The capture EIA yielded 99% of sensitivity and 93% specificity, when compared with the commercial test. The capture EIA developed here is of interest for epidemiological studies, particularly for endemic regions in South America.
authors.affiliation: Laboratorio de Biología de Virus, Universidad Central de Venezuela, Caracas, Venezuela.
authors.forename: N
authors.initials: N
authors.lastname: Uzcátegui
authors.forename: J
authors.initials: J
authors.lastname: Flores
authors.forename: F
authors.initials: F
authors.lastname: Liprandi
authors.forename: F H
authors.initials: FH
authors.lastname: Pujol
mesh_terms: D015153:Blotting, Western; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D006509:Hepatitis B / Q000175:diagnosis; D006510:Hepatitis B Antibodies / Q000032:analysis*; D006512:Hepatitis B Core Antigens / Q000276:immunology*; D006801:Humans; D007075:Immunoglobulin M / Q000032:analysis*; D012680:Sensitivity and Specificity
chemical_list: D006510:Hepatitis B Antibodies; D006512:Hepatitis B Core Antigens; D007075:Immunoglobulin M
vernacular_title: Desarrollo de un ensayo enzimático para la determinación de anticuerpos IgM anti-cápside del virus de la Hepatitis B.