Interaction of transcription factor YY1 with a replication-enhancing element, REE1, in an autonomously replicating human chromosome fragment.
We have previously shown that autonomous replication of human chromosome fragments is stimulated by the presence of an 18 bp sequence, REE1, which exhibits transcriptional silencer activity. The REE1 sequence is partly homologous with the serum response element (SRE) required for expression of the human c- fos gene. Here we have examined interaction of REE1 with human nuclear proteins using a gel retardation assay. One of the REE1-protein complexes formed showed almost the same mobility as the SRE-protein complex and complex formation was competitively inhibited by the SRE fragment. The protein complex with REE1 as well as that with SRE was found to contain the transcription factor YY1, known to bind to the SRE. These results suggest that YY1 protein may participate in stimulation of replication through its interaction with REE1.
Obuse
C
C
Department of Molecular Biology, School of Science, Nagoya University, Nagoya 464-8602, Japan.
Okazaki
T
T
Masukata
H
H
D001483:Base Sequence; D002457:Cell Extracts; D002461:Cell Line, Transformed; D002467:Cell Nucleus; D002877:Chromosomes, Human / Q000235:genetics / Q000378:metabolism; D004247:DNA / Q000235:genetics / Q000378:metabolism*; D004261:DNA Replication / Q000235:genetics*; D004268:DNA-Binding Proteins / Q000378:metabolism*; D050981:Erythroid-Specific DNA-Binding Factors; D006801:Humans; D009687:Nuclear Proteins / Q000378:metabolism; D026362:Serum Response Factor; D014157:Transcription Factors / Q000378:metabolism*; D050986:YY1 Transcription Factor
D002457:Cell Extracts; D004268:DNA-Binding Proteins; D050981:Erythroid-Specific DNA-Binding Factors; D009687:Nuclear Proteins; D026362:Serum Response Factor; D014157:Transcription Factors; D050986:YY1 Transcription Factor; C494698:YY1 protein, human; D004247:DNA