Gamma/delta T cell receptor genes rearrangement in the blood and brain of multiple sclerosis patients. A preliminary study.
The characterization of TCR gamma/delta gene diversity in peripheral blood lymphocytes and in cerebral white matter of MS patients was performed. In overwhelming majority of MS cases the V delta-J delta junctional repertoire was restricted as evidenced by oligoclonal rearrangements concerning V delta 1-J delta 1, V delta 2-J delta 1, V delta 3-J delta 1, V delta 5-J delta 1. The obtained results indicate, that the restricted pattern of TCR gene rearrangement may suggest oligoclonal expansion of certain clones of gamma/delta T cells that may be involved in recognition of putative autoantigen, significant in the etiopathogenesis of MS. The very striking positive correlation between monoclonal pattern of V delta-J delta rearrangement in peripheral blood and cerebral white matter of MS patients indicate that the gamma/delta T lymphocytes play significant role in MS pathogenesis.
Michałowska-Wender
G
G
Neuroimmunological Unit, Medical Research Center, Poznań.
Nowak
J
J
Wender
M
M
D000328:Adult; D001923:Brain Chemistry; D005260:Female; D005625:Frontal Lobe / Q000473:pathology*; D016694:Gene Rearrangement, delta-Chain T-Cell Antigen Receptor / Q000235:genetics*; D006801:Humans; D008297:Male; D008875:Middle Aged; D009103:Multiple Sclerosis / Q000235:genetics* / Q000276:immunology / Q000473:pathology; D016133:Polymerase Chain Reaction; D013601:T-Lymphocytes / Q000737:chemistry*; D013702:Temporal Lobe / Q000473:pathology*