N-benzoyl, L-glutamic acid as a suitable internal standard for the analysis of trans,trans-muconic acid in human urine by liquid chromatography.
Urinary trans,trans-muconic acid is a sensitive biomarker for low level benzene exposure. The method described by Ducos et al. (Int Arch Occup Environ Health 1990; 62:529-34) is commonly used for its determination. In this study, we demonstrate that N-benzoyl, L-glutamic acid added to urine samples is a suitable internal standard to control trans,trans-muconic acid recovery after solid phase extraction of urine and to compensate for variations which might occur during high-performance liquid chromatography analysis.
Renom
G
G
Laboratoire du Comité pour le Développement de la Médecine du Travail, Lille, France.
Bruneau
N
N
Mizon
J
J
D001554:Benzene; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D018698:Glutamic Acid; D006801:Humans; D012015:Reference Standards; D013011:Sorbic Acid / Q000031:analogs & derivatives* / Q000032:analysis; D016482:Urinalysis / Q000379:methods
C005939:muconic acid; D018698:Glutamic Acid; D001554:Benzene; D013011:Sorbic Acid