Development of more efficacious antibodies for medical therapy and diagnosis.
Two procedures for improving the efficacy of medically important antibodies are described. The first procedure is designed to reduce the immunogenicity of nonhuman antibodies to the barest minimum--the "humanization" is accomplished by transplanting only the specificity-determining residues of the nonhuman antibody onto a human antibody template. The second procedure is designed to permit the easy production of multispecific/multivalent antibodies via heterodimer formation of electrostatically complementary Fc regions.
Santos
A D
AD
Laboratory of Molecular Biology, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, Maryland 20892, USA.
Padlan
E A
EA
D000595:Amino Acid Sequence; D000818:Animals; D000906:Antibodies / Q000737:chemistry / Q000627:therapeutic use*; D000918:Antibody Specificity; D015195:Drug Design; D006801:Humans; D007135:Immunoglobulin Variable Region; D008958:Models, Molecular; D008969:Molecular Sequence Data; D011487:Protein Conformation; D011994:Recombinant Proteins / Q000737:chemistry / Q000627:therapeutic use; D013045:Species Specificity
D000906:Antibodies; D007135:Immunoglobulin Variable Region; D011994:Recombinant Proteins