title: Effect of basic fibroblast growth factor on cholecystokinin-induced amylase release and intracellular calcium increase in male rat pancreatic acinar cells.
abstract: Isolated rat pancreatic acinar cells were used to investigate the effect of basic fibroblast growth factor (bFGF) on both amylase secretion and intracellular free calcium concentration ([Ca2+]i) in response to the calcium-mobilizing secretagogue cholecystokinin-octapeptide (CCK-8). Our data show that bFGF inhibited CCK-8-induced amylase release in a concentration-dependent manner and decreased the CCK-8-induced rise in [Ca2+]i. This inhibitory effect of bFGF on both amylase secretion and [Ca2+]i increase in response to CCK-8 was reverted when acinar cells were pretreated with 100 microM tyrphostin A25, a tyrosine kinase inhibitor. Tyrphostin A25 also inhibited Ca2+ influx induced by CCK-8. These results show that bFGF inhibits CCK-8-induced pancreatic response by a tyrosine kinase-dependent mechanism. A role for tyrosine phosphorylation in capacitative Ca2+ entry is suggested.
authors.affiliation: Department of Physiology, Faculty of Veterinary Science, University of Extremadura, Cáceres, Spain.
authors.forename: A I
authors.initials: AI
authors.lastname: Lajas
authors.forename: M J
authors.initials: MJ
authors.lastname: Pozo
authors.forename: G M
authors.initials: GM
authors.lastname: Salido
authors.forename: J A
authors.initials: JA
authors.lastname: Pariente
mesh_terms: D000681:Amylases / Q000378:metabolism*; D000818:Animals; D002118:Calcium / Q000378:metabolism*; D004791:Enzyme Inhibitors / Q000494:pharmacology; D016222:Fibroblast Growth Factor 2 / Q000494:pharmacology*; D008297:Male; D009570:Nitriles / Q000494:pharmacology; D010179:Pancreas / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D011505:Protein-Tyrosine Kinases / Q000037:antagonists & inhibitors; D051381:Rats; D017208:Rats, Wistar; D012844:Sincalide / Q000494:pharmacology*; D020032:Tyrphostins
chemical_list: D004791:Enzyme Inhibitors; D009570:Nitriles; D020032:Tyrphostins; D016222:Fibroblast Growth Factor 2; C079836:tyrphostin 25; D011505:Protein-Tyrosine Kinases; D000681:Amylases; D012844:Sincalide; D002118:Calcium