Both IFN-gamma and IL-4 induce MHC class II expression at the surface of mouse pro-B cells.
Pro-B cells are early B-cell progenitors that retain macrophage potential. We have studied MHC class II molecules and invariant chain inducibility on four class II negative mouse pro-B-cell clones. We analyzed the effects of IL-4 and IFN-gamma, which represent the major inducers of class II in the B-lymphoid and monocytic/macrophage lineages, respectively. After 48 h of treatment with either cytokine, three pro-B-cell clones (C2.13, A1.5, and F2.2) expressed intracellular invariant chain and cell-surface class II molecules. One clone (D2.1) remained negative. As already reported, more differentiated 70Z/3 pre-B cells were inducible by IL-4 only. These data suggest that the induction of class II and invariant-chain genes are subject to regulation throughout B-cell differentiation.
Lombard-Platet
S
S
Laboratoire de Génétique Moléculaire des Eucaryotes du CNRS, Strasbourg, France.
Meyer
V
V
Ceredig
R
R
D000818:Animals; D001402:B-Lymphocytes / Q000166:cytology / Q000276:immunology*; D002454:Cell Differentiation; D002460:Cell Line; D018076:DNA, Complementary; D005434:Flow Cytometry; D005786:Gene Expression Regulation; D005802:Genes, MHC Class II / Q000235:genetics*; D006412:Hematopoietic Stem Cells / Q000166:cytology / Q000276:immunology*; D007371:Interferon-gamma / Q000494:pharmacology*; D015847:Interleukin-4 / Q000494:pharmacology*; D051379:Mice; D008822:Mice, Transgenic; D016133:Polymerase Chain Reaction; D012313:RNA / Q000302:isolation & purification; D014158:Transcription, Genetic
D018076:DNA, Complementary; D015847:Interleukin-4; D012313:RNA; D007371:Interferon-gamma