Analyses of monoclonal antibodies reacting with porcine wCD6: results from the Second International Swine CD Workshop.
Among the 57 monoclonal antibodies analyzed within the T-cell group of the Second International Swine CD Workshop, one mAb fell within cluster T14a that included the CD6 standard a38b2 (No. 175). The new mAb MIL8 (No. 082) and a38b2 both precipitated from activated T-cells a 150 kDa monomeric protein. Staining patterns on the various cell types were similar. There was no inhibition of binding of either mAb to peripheral blood T-cells with the opposite mAb. The new mAb, MIL8, reacts with a separate epitope on porcine wCD6.
Pescovitz
M D
MD
Indiana University, Dept. of Surgery and Microbiology/Immunology, Indianapolis 46202, USA.
Book
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BK
Aasted
B
B
Dominguez
J
J
Bullido
R
R
Trebichavsky
I
I
Novikov
B
B
Valpotic
I
I
Nielsen
J
J
Arn
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Sachs
D H
DH
Lunney
J K
JK
Boyd
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Walker
J
J
Lee
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Petrinec
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Saalmüller
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D000818:Animals; D000911:Antibodies, Monoclonal / Q000032:analysis*; D000937:Antigen-Antibody Reactions; D015703:Antigens, CD / Q000096:biosynthesis / Q000276:immunology*; D000945:Antigens, Differentiation, T-Lymphocyte / Q000096:biosynthesis / Q000276:immunology*; D005434:Flow Cytometry; D013552:Swine / Q000276:immunology*; D016176:T-Lymphocyte Subsets / Q000276:immunology / Q000378:metabolism
D000911:Antibodies, Monoclonal; D015703:Antigens, CD; D000945:Antigens, Differentiation, T-Lymphocyte; C062279:CD6 antigen