title: Synthesis of guanosine 5'-triphosphate analogues and their effect on microtubule assembly.
abstract: More than 20 base-modified analogues of guanosine 5'-triphosphate including 2'-deoxyguanosine derivatives were synthesized and examined their effect on tubulin polymerization into microtubules (Mts). Among those, 2,6-diamino-8-oxopurineriboside 5'-triphosphate (1d), 2,6-diamino-2'-deoxypurineriboside 5'-tri phosphate (1b) and 8-bromoguanosine 5'-tri phosphate (1g) were shown to have remarkable effect to promote microtubule assembly.
authors.affiliation: Department of Chemical and Biological Sciences, Faculty of Science, Japan Women's University, Tokyo, Japan.
authors.forename: M
authors.initials: M
authors.lastname: Muraoka
authors.forename: H
authors.initials: H
authors.lastname: Fukuzawa
authors.forename: A
authors.initials: A
authors.lastname: Nisita
authors.forename: K
authors.initials: K
authors.lastname: Okano
authors.forename: T
authors.initials: T
authors.lastname: Tsuchihara
authors.forename: H
authors.initials: H
authors.lastname: Sakai
mesh_terms: D006160:Guanosine Triphosphate / Q000031:analogs & derivatives* / Q000138:chemical synthesis / Q000737:chemistry / Q000494:pharmacology*; D007700:Kinetics; D008870:Microtubules / Q000187:drug effects* / Q000502:physiology; D015394:Molecular Structure; D013329:Structure-Activity Relationship; D014404:Tubulin / Q000187:drug effects / Q000378:metabolism*
chemical_list: D014404:Tubulin; D006160:Guanosine Triphosphate