Identification of an Na+-dependent malonate transporter of Malonomonas rubra and its dependence on two separate genes.
Two membrane proteins encoded by the malonate fermentation gene cluster of Malonomonas rubra, MadL and MadM, have been synthesized in Escherichia coli. MadL and MadM were shown to function together as a malonate transport system, whereas each protein alone was unable to catalyze malonate transport. Malonate transport by MadLM is Na+ dependent, and imposition of a DeltapNa+ markedly enhanced the rate of malonate uptake. The kinetics of malonate uptake into E. coli BL21(DE3) cells synthesizing MadLM at different pH values indicated that Hmalonate- is the transported malonate species. The stimulation of malonate uptake by Na+ ions showed Michaelis-Menten kinetics, and a Km for Na+ of 1.2 mM was determined. These results suggest that MadLM is an electroneutral Na+/Hmalonate- symporter and that it is dependent on two separate genes.
Schaffitzel
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Mikrobiologisches Institut, Eidgenössische Technische Hochschule, Zürich, Switzerland.
Berg
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Dimroth
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D001692:Biological Transport; D005798:Genes, Bacterial / Q000235:genetics*; D006089:Gram-Negative Anaerobic Bacteria / Q000235:genetics* / Q000378:metabolism*; D008314:Malonates / Q000378:metabolism*; D011188:Potassium / Q000378:metabolism
D008314:Malonates; D011188:Potassium