title: Ontogeny and cellular localization of the pyruvate recycling system in rat brain.
abstract: The ontogeny of the cerebral pyruvate recycling pathway and the cellular localization of associated enzymes, malic enzyme (ME) and phosphoenolpyruvate carboxykinase (PEPCK), have been investigated using a combination of 13C NMR spectroscopy, enzymatic analysis, and molecular biology approaches. Activity of the pathway, using [1,2-(13)C2]acetate as a substrate, was detected by 13C NMR in brain extracts 3 weeks after birth, increasing progressively up to the third month of age. In whole-brain homogenates, ME activity increased to adult levels with the same time course as the recycling pathway. PEPCK activity was low during the first 2 weeks of life and decreased further toward adulthood. ME and PEPCK activity were found in primary cultures of astrocytes and in synaptosomal fractions of adult brain. Primary cultures of cortical neurons showed PEPCK activity but no detectable ME activity. The cytosolic ME gene was expressed in primary cultures of neurons and in astrocytes as well as in the neonatal and adult brain. The PEPCK gene was expressed both in primary cultures of cortical neurons and in astrocytes, but the level of its expression in the neonatal and adult brain was undetectable.
authors.affiliation: Instituto de Investigaciones Biomédicas, CSIC, Madrid, Spain.
authors.forename: F
authors.initials: F
authors.lastname: Cruz
authors.forename: S R
authors.initials: SR
authors.lastname: Scott
authors.forename: I
authors.initials: I
authors.lastname: Barroso
authors.forename: P
authors.initials: P
authors.lastname: Santisteban
authors.forename: S
authors.initials: S
authors.lastname: Cerdán
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D001253:Astrocytes / Q000201:enzymology; D001921:Brain / Q000201:enzymology / Q000254:growth & development / Q000378:metabolism* / Q000648:ultrastructure; D002478:Cells, Cultured; D009682:Magnetic Resonance Spectroscopy; D008291:Malate Dehydrogenase / Q000096:biosynthesis / Q000378:metabolism* / Q000648:ultrastructure; D009474:Neurons / Q000201:enzymology; D009928:Organ Specificity; D010729:Phosphoenolpyruvate Carboxykinase (GTP) / Q000096:biosynthesis / Q000378:metabolism* / Q000648:ultrastructure; D019289:Pyruvic Acid / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar; D013574:Synaptosomes / Q000201:enzymology; D014407:Tumor Cells, Cultured
chemical_list: D019289:Pyruvic Acid; D008291:Malate Dehydrogenase; C031917:malate dehydrogenase (oxaloacetate-decarboxylating) (NADP+); D010729:Phosphoenolpyruvate Carboxykinase (GTP)