Radioimmunoimaging of arterial and venous thrombi in canine model using 99mTc labeled monoclonal antifibrin antibody.
OBJECTIVE
To evaluate the usefulness of 99mTc labelled monoclonal antifibrin antibody SZ-63 for the detection of vascular thrombi, and to investigate the feasibility of thrombus imaging 99mTc-SZ-63.


METHODS
The monoclonal antibody SZ-63 (Mab SZ-63), which reacts with D-dimer, XLFN and fibrin monomer was produced and labelled with 99mTc using pretinning procedure. After that, the femoral arterial or venous thrombus model was prepared in dogs and injected with 99mTc-SZ-63 intravenously. Both arterial and venous thrombus models were imaged.


RESULTS
Both the arterial and venous thrombi were clearly visible within 2.5 hours after injection of 99mTc-SZ-63. Quantitative imaging analysis showed that the ratios between the thrombus and the opposite vessel increased with time extension. The ratios of thrombus to blood or surrounding muscle were 2. 56 +/- 0.83 and 13.40 +/- 5.10 for arterial thrombi and 3.34 +/- 1.06 and 22.40 +/- 6.40 for venous thrombi after the canines were killed.


CONCLUSIONS
99mTc-SZ-63 is a useful imaging agent for thrombus detection in vivo.
Ruan
C
C
Jiangsu Institute of Hematology, Thrombosis and Hemostasis Research Unit, Suzhou Medical College, China.
Wang
Q
Q
He
G
G
Wu
J
J
D000818:Animals; D000911:Antibodies, Monoclonal; D004285:Dogs; D005263:Femoral Artery / Q000000981:diagnostic imaging; D005268:Femoral Vein / Q000000981:diagnostic imaging; D005337:Fibrin / Q000276:immunology*; D006168:Guinea Pigs; D051379:Mice; D008807:Mice, Inbred BALB C; D011817:Rabbits; D016719:Radioimmunodetection; D013667:Technetium; D013927:Thrombosis / Q000000981:diagnostic imaging*
D000911:Antibodies, Monoclonal; D013667:Technetium; D005337:Fibrin