title: Somatic cell cloned transgenic bovine neurons for transplantation in parkinsonian rats.
abstract: Parkinson's disease symptoms can be improved by transplanting fetal dopamine cells into the putamen of parkinsonian patients. Because the supply of human donor tissue is limited and variable, an alternative and genetically modifiable non-human source of tissue would be valuable. We have generated cloned transgenic bovine embryos, 42% of which developed beyond 40 days. Dopamine cells collected from the ventral mesencephalon of the cloned fetuses 42 to 50 days post-conception survived transplantation into immunosuppressed parkinsonian rats and cells from cloned and wild-type embryos improved motor performance. Somatic cell cloning can efficiently produce transgenic animal tissue for treating parkinsonism.
authors.affiliation: Department of Medicine, University of Colorado School of Medicine, Denver 80262, USA.
authors.forename: W M
authors.initials: WM
authors.lastname: Zawada
authors.forename: J B
authors.initials: JB
authors.lastname: Cibelli
authors.forename: P K
authors.initials: PK
authors.lastname: Choi
authors.forename: E D
authors.initials: ED
authors.lastname: Clarkson
authors.forename: P J
authors.initials: PJ
authors.lastname: Golueke
authors.forename: S E
authors.initials: SE
authors.lastname: Witta
authors.forename: K P
authors.initials: KP
authors.lastname: Bell
authors.forename: J
authors.initials: J
authors.lastname: Kane
authors.forename: F A
authors.initials: FA
authors.lastname: Ponce de Leon
authors.forename: D J
authors.initials: DJ
authors.lastname: Jerry
authors.forename: J M
authors.initials: JM
authors.lastname: Robl
authors.forename: C R
authors.initials: CR
authors.lastname: Freed
authors.forename: S L
authors.initials: SL
authors.lastname: Stice
mesh_terms: D000818:Animals; D030801:Animals, Genetically Modified; D002417:Cattle; D019976:Cloning, Organism; D004298:Dopamine / Q000096:biosynthesis*; D004628:Embryonic Structures / Q000637:transplantation; D007763:Lac Operon; D008636:Mesencephalon / Q000196:embryology / Q000637:transplantation; D009474:Neurons / Q000637:transplantation*; D010300:Parkinson Disease / Q000628:therapy*; D051381:Rats; D014183:Transplantation, Heterologous / Q000379:methods*
chemical_list: D004298:Dopamine