Endothelins inhibit mineralization of rat calvarial osteoblast-like cells.
We examined the effects of members of the endothelin (ET) family on mineralization of rat calvarial osteoblast-like cells. The accumulation of calcium in cells and cell layers was attenuated by ETs with the rank order of potency ET-1 = ET-2 > ET-3. We stained the mineralized nodules by von Kossa staining and measured the number and area of mineralized nodules. The inhibitory effects of ET-1 and ET-2 on the formation of mineralized nodules were stronger than those of ET-3. Our data suggest that ET-1 may inhibit the mineralization process of osteoblastic cells through the ETA receptor.
Hiruma
Y
Y
Research Center for Experimental Biology, Tokyo Institute of Technology, Yokohama, Japan.
Inoue
A
A
Shiohama
A
A
Otsuka
E
E
Hirose
S
S
Yamaguchi
A
A
Hagiwara
H
H
D000818:Animals; D000831:Animals, Newborn; D002113:Calcification, Physiologic / Q000187:drug effects*; D002118:Calcium / Q000378:metabolism; D002478:Cells, Cultured; D065128:Endothelin Receptor Antagonists; D016232:Endothelins / Q000494:pharmacology*; D006801:Humans; D010006:Osteoblasts / Q000187:drug effects* / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D044022:Receptor, Endothelin A; D012886:Skull / Q000166:cytology / Q000187:drug effects*
D065128:Endothelin Receptor Antagonists; D016232:Endothelins; D044022:Receptor, Endothelin A; D002118:Calcium