Preliminary studies for interaction of a cyclobutane thymine dimer region with its cognate antibody.
A monoclonal antibody (TDM-2) specific to a UV-induced cyclobutane pyrimidine dimer has previously been established. We investigated here the structural requirements of antigen recognition by the antibody using chemically synthesized antigen analogs. TDM-2 bound to the cis-syn, but not the trans-syn thymine dimer, and recognized not only the cyclobutane ring but also the 5'- or 3'-side phosphate groups flanking the cyclobutane dimer site. The antibody showed strong binding to photodimer-containing single stranded DNA but indicated little binding to double stranded DNA.
Komatsu
Y
Y
Faculty of Pharmaceutical Sciences, Hokkaido University, Sapporo, Japan.
Tujino
T
T
Suzuki
T
T
Nikaido
O
O
Ohtsuka
E
E
D000911:Antibodies, Monoclonal; D001666:Binding Sites, Antibody; D004247:DNA / Q000737:chemistry* / Q000276:immunology / Q000528:radiation effects; D004277:DNA, Single-Stranded / Q000737:chemistry / Q000276:immunology / Q000528:radiation effects; D011740:Pyrimidine Dimers / Q000138:chemical synthesis / Q000737:chemistry* / Q000276:immunology*; D013237:Stereoisomerism; D014466:Ultraviolet Rays
D000911:Antibodies, Monoclonal; D004277:DNA, Single-Stranded; D011740:Pyrimidine Dimers; D004247:DNA