title: Immunohistochemical evidence for a macrophage scavenger receptor in Mato cells and reactive microglia of ischemia and Alzheimer's disease.
abstract: Macrophage scavenger receptors (MSR) are implicated in the development of atherosclerosis and amyloid b-protein deposition in Alzheimer's disease. However, histopathological studies of MSR expression in human tissues have been hampered by a lack of specific antibodies. Using MSR-deficient mice, we successfully raised a novel monoclonal antibody against human MSR together with high-titer antisera. These antibodies specifically recognized human tissue macrophages and human MSR protein purified from differentiated THP1 cells. In normal brain, MSR staining was mainly distributed to the perivascular cells, which correspond to Mato's fluorescent granular perithelial cells (Mato cells). In the lesions of ischemia and Alzheimer's disease, a subset of microglia stained positive for MSR. These novel antibodies are useful tools for analysis of MSR expression in human tissues.
authors.affiliation: Department of Neuropsychiatry, University of Tokyo, Japan.
authors.forename: M
authors.initials: M
authors.lastname: Honda
authors.forename: H
authors.initials: H
authors.lastname: Akiyama
authors.forename: Y
authors.initials: Y
authors.lastname: Yamada
authors.forename: H
authors.initials: H
authors.lastname: Kondo
authors.forename: Y
authors.initials: Y
authors.lastname: Kawabe
authors.forename: M
authors.initials: M
authors.lastname: Takeya
authors.forename: K
authors.initials: K
authors.lastname: Takahashi
authors.forename: H
authors.initials: H
authors.lastname: Suzuki
authors.forename: T
authors.initials: T
authors.lastname: Doi
authors.forename: A
authors.initials: A
authors.lastname: Sakamoto
authors.forename: S
authors.initials: S
authors.lastname: Ookawara
authors.forename: M
authors.initials: M
authors.lastname: Mato
authors.forename: P J
authors.initials: PJ
authors.lastname: Gough
authors.forename: D R
authors.initials: DR
authors.lastname: Greaves
authors.forename: S
authors.initials: S
authors.lastname: Gordon
authors.forename: T
authors.initials: T
authors.lastname: Kodama
authors.forename: M
authors.initials: M
authors.lastname: Matsushita
mesh_terms: D000544:Alzheimer Disease / Q000378:metabolism*; D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D001921:Brain / Q000378:metabolism / Q000473:pathology; D015815:Cell Adhesion Molecules / Q000378:metabolism*; D002460:Cell Line; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D007150:Immunohistochemistry; D007511:Ischemia / Q000378:metabolism*; D051379:Mice; D017628:Microglia / Q000378:metabolism*; D011971:Receptors, Immunologic / Q000276:immunology / Q000378:metabolism*; D051116:Receptors, Scavenger
chemical_list: D000911:Antibodies, Monoclonal; D015815:Cell Adhesion Molecules; D011971:Receptors, Immunologic; D051116:Receptors, Scavenger