Inhibitory effect of ethanol administration on beta-alanine-2-oxoglutarate aminotransferase (GABA aminotransferase) in disulfiram-pretreated rats.
Ethanol in the presence of disulfiram (N,N,N',N'-tetraethylthiuram disulfide, an inhibitor of aldehyde dehydrogenase) inhibited liver beta-alanine-oxoglutarate aminotransferase (beta-AlaAT I) activity yet activated tyrosine aminotransferase (TAT) in weanling rats in vivo. The effect on beta-AlaAT I was followed by the inhibitory expression of beta-AlaAT I mRNA. The beta-AlaAT I activity was reduced with a pseudo-first-order profile with time, and the half-life was calculated to be 12.3 +/- 0.83 h with the rate constant (Kd) of 0.056 +/- 0.004 h-1. The synthesis of beta-AlaAT I in rat liver was estimated to be 1.56 x 10(-10) mol/g of wet tissue per hour at a steady state. A combination of ethanol and disulfiram also reduced beta-alanine-pyruvate aminotransferase (beta-AlaAT II) activity to 60% of the control after 24 h.
Kontani
Y
Y
Faculty of Nutrition, Kobe-Gakuin University, Japan.
Kawasaki
S
S
Kaneko
M
M
Matsuda
K
K
Sakata
S F
SF
Tamaki
N
N
D000612:4-Aminobutyrate Transaminase / Q000037:antagonists & inhibitors*; D000410:Alanine Transaminase / Q000037:antagonists & inhibitors; D000427:Alcohol Deterrents / Q000494:pharmacology*; D000444:Aldehyde Dehydrogenase / Q000037:antagonists & inhibitors; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D004221:Disulfiram / Q000494:pharmacology*; D004791:Enzyme Inhibitors / Q000494:pharmacology*; D000431:Ethanol / Q000494:pharmacology*; D007700:Kinetics; D008297:Male; D008969:Molecular Sequence Data; D051381:Rats; D017208:Rats, Wistar; D000637:Transaminases / Q000037:antagonists & inhibitors; D014444:Tyrosine Transaminase / Q000037:antagonists & inhibitors; D014886:Weaning
D000427:Alcohol Deterrents; D004791:Enzyme Inhibitors; D000431:Ethanol; D000444:Aldehyde Dehydrogenase; D000637:Transaminases; D000612:4-Aminobutyrate Transaminase; D000410:Alanine Transaminase; C019685:D-3-aminoisobutyrate-pyruvate aminotransferase; D014444:Tyrosine Transaminase; D004221:Disulfiram