title: T cell subsets and T cell function in cartilage-hair hypoplasia.
abstract: Cartilage hair hypoplasia is a rare autosomal recessive form of short-limbed dwarfism associated with a cellular immunodeficiency. In eight patients, the authors studied the presence of T cell subsets and in vitro T cell function in order to address the basis for the immunological disorder. Both the proliferative response to phytohaemagglutinin (PHA) and the PHA-induced IL2 production were 60% lower compared with controls (P = 0.007 and 0.005, respectively). The impaired proliferative response could not be restored by addition of IL-2. This result is in accordance with a decrease in the percentage of activated T cells expressing the p55 subunit of the IL-2 receptor complex (CD25). The results define more precisely that T cells from cartilage hair hypoplasia patients are defective in the transition from the G0 to the G1 phase of the cell cycle. Furthermore, the data demonstrate that several CHH patients show a reduced proportion of CD45RA+ 'naive' T cells. However, the in vitro impairment of T cell function cannot solely be explained by imbalance between 'naive' and 'memory' T cells. Although CHH patients with a history of recurrent respiratory tract infections showed the most aberrant in vitro immune parameters, a clear relationship between clinical data and in vitro parameters could not be established for the whole patient group.
authors.affiliation: Department of Immunology, University Hospital for Children and Youth, Utrecht, The Netherlands.
authors.forename: R
authors.initials: R
authors.lastname: Kooijman
authors.forename: C J
authors.initials: CJ
authors.lastname: van der Burgt
authors.forename: C M
authors.initials: CM
authors.lastname: Weemaes
authors.forename: A
authors.initials: A
authors.lastname: Haraldsson
authors.forename: E J
authors.initials: EJ
authors.lastname: Scholtens
authors.forename: B J
authors.initials: BJ
authors.lastname: Zegers
mesh_terms: D000328:Adult; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology*; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology*; D002648:Child; D004247:DNA / Q000096:biosynthesis; D004261:DNA Replication; D005260:Female; D006801:Humans; D007376:Interleukin-2 / Q000378:metabolism; D037102:Lectins; D017493:Leukocyte Common Antigens / Q000378:metabolism; D008213:Lymphocyte Activation / Q000276:immunology; D008297:Male; D008875:Middle Aged; D010009:Osteochondrodysplasias / Q000276:immunology*; D015375:Receptors, Interleukin-2 / Q000378:metabolism; D011990:Receptors, Transferrin / Q000378:metabolism; D016176:T-Lymphocyte Subsets / Q000276:immunology
chemical_list: D007376:Interleukin-2; D037102:Lectins; D015375:Receptors, Interleukin-2; D011990:Receptors, Transferrin; D004247:DNA; D017493:Leukocyte Common Antigens