Stimulatory effect of paeoniflorin on adenosine A-1 receptors to increase the translocation of protein kinase C (PKC) and glucose transporter (GLUT 4) in isolated rat white adipocytes.
In an attempt to understand the subcellular signals after activation of adenosine A-1 receptors, paeoniflorin was employed to incubate with rat white adipocytes in vitro. Translocation of protein kinase C (PKC) beta-subtype from cytosol to membrane was enhanced by an incubation with paeoniflorin in a concentration-dependent manner similar to that of porcine insulin. Also, 8-cyclopentyl-1,3-dipropylxanthine (DPCPX) inhibited this action of paeoniflorin in a concentration-related fashion and it markedly attenuated the action of paeoniflorin at a concentrations sufficient to block the action of adenosine. Moreover, chelerythrine inhibited the paeoniflorin-stimulated translocation of PKC in a way similar to that stimulated by porcine insulin. Subcellular inhibition is considered because stimulation of porcine insulin was not modified by DPCPX at concentrations sufficient to block adenosine A-1 receptors. Similar results were also observed in adipocytes regarding the translocation of glucose transporter (GLUT4) from cytosol to membrane. Thus, we found that paeoniflorin can activate adenosine A-1 receptors to increase the translocations of PKC and GLUT4, two major signals for glucose uptake, from cytosol to membrane of the white adipocytes in rats.
Lai
C W
CW
Department of Pharmacology, College of Medicine, National Cheng Kung University, Tainan City, Taiwan, Republic of China.
Hsu
F L
FL
Cheng
J T
JT
D017667:Adipocytes / Q000187:drug effects* / Q000378:metabolism*; D000470:Alkaloids; D000818:Animals; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000037:antagonists & inhibitors / Q000494:pharmacology*; D001565:Benzoates; D053119:Benzophenanthridines; D001952:Bridged-Ring Compounds; D002462:Cell Membrane / Q000378:metabolism; D003600:Cytosol / Q000378:metabolism; D004347:Drug Interactions; D004791:Enzyme Inhibitors / Q000494:pharmacology; D051275:Glucose Transporter Type 4; D005960:Glucosides / Q000037:antagonists & inhibitors / Q000494:pharmacology*; D007527:Isoenzymes / Q000378:metabolism*; D009004:Monosaccharide Transport Proteins / Q000378:metabolism*; D039821:Monoterpenes; D009124:Muscle Proteins; D010617:Phenanthridines / Q000494:pharmacology; D010766:Phosphorylation / Q000187:drug effects; D011493:Protein Kinase C / Q000378:metabolism*; D064546:Protein Kinase C beta; D058906:Purinergic P1 Receptor Agonists; D051381:Rats; D017208:Rats, Wistar; D015398:Signal Transduction / Q000187:drug effects / Q000502:physiology; D013268:Stimulation, Chemical; D014970:Xanthines / Q000494:pharmacology; D006027:Glycosides
D000470:Alkaloids; D000894:Anti-Inflammatory Agents, Non-Steroidal; D001565:Benzoates; D053119:Benzophenanthridines; D001952:Bridged-Ring Compounds; D004791:Enzyme Inhibitors; D051275:Glucose Transporter Type 4; D005960:Glucosides; D007527:Isoenzymes; D009004:Monosaccharide Transport Proteins; D039821:Monoterpenes; D009124:Muscle Proteins; D010617:Phenanthridines; D011493:Protein Kinase C; D064546:Protein Kinase C beta; D058906:Purinergic P1 Receptor Agonists; D014970:Xanthines; C495694:Slc2a4 protein, rat; C015423:peoniflorin; D006027:Glycosides; C051360:1,3-dipropyl-8-cyclopentylxanthine; C016299:chelerythrine