title: GB virus C prevalence in blood donors and high risk groups for parenterally transmitted agents from Gauteng, South Africa.
abstract: The prevalence of GBV-C infection in voluntary blood donors and in groups at high risk for parenteral exposure to infectious agents was studied. The high risk groups included chronic renal failure patients on haemodialysis, renal transplant patients and haemophiliacs from Gauteng. The presence of GBV-C RNA in these populations was determined using reverse transcription polymerase chain reaction (RT-PCR) in the 5' non-coding region (NCR) of the virus. Of the blood donors, 11.1% (95% CI 7.6, 15.8) were positive, whereas 23.8% (95% CI 12.6, 40.2) of haemodialysis patients and 23.5% (95% CI 15.9, 33.3) of the haemophiliacs were infected with GBV-C. The highest proportion of infection was in the renal transplant patients, where 41.2% (95% CI 35.1, 47.7) were found to have circulating GBV-C RNA. Serological markers for hepatitis B (HBV) and hepatitis C viruses (HCV) were also measured as indicators of other hepatitis viruses with important parenteral transmission routes. Of the GBV-C positive blood donors, 3.6% were also HBsAg positive and none were positive for HCV. The GBV-C positive patients on haemodialysis were not positive for either HBsAg or antibodies to HCV, but had evidence of past infection with HBV since 40% were anti-HBc positive. The greatest proportion of HCV positives was in the haemophiliac group, 91.3%, none of these were HBsAg positive but 39.1% had anti-HBc. In the GBV-C positive renal transplant patients, 4% had HBsAg, 13.3% had anti-HBc and 2.1% had antibodies to HCV. This is the first report describing the prevalence of GBV-C in South African populations.
authors.affiliation: National Institute for Virology, University of the Witwatersrand, Department of Virology, Sandringham, South Africa.
authors.forename: A
authors.initials: A
authors.lastname: Casteling
authors.forename: E
authors.initials: E
authors.lastname: Song
authors.forename: J
authors.initials: J
authors.lastname: Sim
authors.forename: D
authors.initials: D
authors.lastname: Blaauw
authors.forename: A
authors.initials: A
authors.lastname: Heyns
authors.forename: R
authors.initials: R
authors.lastname: Schweizer
authors.forename: L
authors.initials: L
authors.lastname: Margolius
authors.forename: E
authors.initials: E
authors.lastname: Kuun
authors.forename: S
authors.initials: S
authors.lastname: Field
authors.forename: B
authors.initials: B
authors.lastname: Schoub
authors.forename: E
authors.initials: E
authors.lastname: Vardas
mesh_terms: D001782:Blood Donors; D005260:Female; D018067:Flaviviridae / Q000235:genetics; D006467:Hemophilia A / Q000821:virology; D006509:Hepatitis B / Q000150:complications; D006526:Hepatitis C / Q000150:complications; D006525:Hepatitis, Viral, Human / Q000097:blood / Q000150:complications / Q000453:epidemiology* / Q000821:virology; D006801:Humans; D007676:Kidney Failure, Chronic / Q000821:virology; D016030:Kidney Transplantation; D008297:Male; D015995:Prevalence; D006435:Renal Dialysis; D012307:Risk Factors; D013019:South Africa / Q000453:epidemiology