HPLC-fluorescence determination of chlorocresol and chloroxylenol in pharmaceuticals.
The use of 2-chloro-6,7-dimethoxy-3-quinolinecarboxaldehyde as a fluorogenic labelling reagent in pre-column derivatization for the HPLC separation of chlorophenols has been investigated. The compound reacts (50 min at 110 degrees C) with 2- and 4-chlorophenols to give fluorescent ethers that can be separated by reversed-phase HPLC and detected at lambda exc = 360 nm, lambda em = 500 nm. The experimental conditions for derivatization and chromatographic separation are discussed. Applications for the determination of chlorocresol (4-chloro-3-cresol) and chloroxylenol (4-chloro-3,5-xylenol) in pharmaceutical formulations (creams, ointments) are described.
Gatti
R
R
Dipartimento di Scienze Farmaceutiche, Università di Bologna, Italy.
Roveri
P
P
Bonazzi
D
D
Cavrini
V
V
D002138:Calibration; D002851:Chromatography, High Pressure Liquid; D003408:Cresols / Q000032:analysis*; D005456:Fluorescent Dyes; D009682:Magnetic Resonance Spectroscopy; D009824:Ointments; D011804:Quinolines; D013050:Spectrometry, Fluorescence; D013055:Spectrophotometry, Infrared; D014992:Xylenes / Q000032:analysis*
C113024:2-chloro-6,7-dimethoxy-3-quinolinecarboxaldehyde; D003408:Cresols; D005456:Fluorescent Dyes; D009824:Ointments; D011804:Quinolines; D014992:Xylenes; C007027:chloroxylenol; C006984:chlorocresol