Synthesis and anti-influenza virus-A activity of circular dumbbell RNA DNA chimeric oligonucleotides.
We have designed a new type of antisense oligonucleotide, containing two hairpin loop structures with RNA/DNA base pairs (sense (RNA) and antisense (DNA)) in the double helical stem (nicked and circular dumbbell DNA/RNA chimeric oligonucleotides). The reaction of the nicked and circular dumbbell DNA/RNA chimeric oligonucleotides with RNase H gave the corresponding anti-DNA together with the sense RNA cleavage products. These oligonucleotides were more resistant to exonuclease attack. We also describe the anti-Fluv activities of circular dumbbell DNA/RNA chimeric oligonucleotides.
Abe
T
T
Department of Industrial Chemistry, Chiba Institute of Technology, Japan.
Hatta
T
T
Yamakawa
H
H
Takai
K
K
Yokota
T
T
Takaku
H
H
D000998:Antiviral Agents / Q000138:chemical synthesis / Q000737:chemistry / Q000494:pharmacology*; D001483:Base Sequence; D002999:Clone Cells; D004247:DNA / Q000737:chemistry; D009980:Influenza A virus / Q000187:drug effects*; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation; D009693:Nucleic Acid Hybridization; D009838:Oligodeoxyribonucleotides / Q000737:chemistry; D016376:Oligonucleotides, Antisense / Q000138:chemical synthesis / Q000737:chemistry / Q000494:pharmacology*; D009843:Oligoribonucleotides / Q000737:chemistry; D012313:RNA / Q000737:chemistry; D016914:Ribonuclease H / Q000378:metabolism
D000998:Antiviral Agents; D009838:Oligodeoxyribonucleotides; D016376:Oligonucleotides, Antisense; D009843:Oligoribonucleotides; D012313:RNA; D004247:DNA; D016914:Ribonuclease H