Mapping of Drosophila mutations using site-specific male recombination.
Although recombination does not usually occur in the male Drosophila germline, site-specific recombination can be induced at the ends of P elements. This finding suggested that male recombination could be used to map Drosophila mutations. In this article, we describe the general method and its application to the mapping of two EMS-induced female-sterile mutations, grauzone and cortex. Within two months, the grauzone gene was mapped relative to seven different P-element insertion sites, and cortex was mapped relative to 23 different P-elements. The results allowed us to map grauzone to a region of about 50 kb, and cortex distal to the chromosomal region 33E. These experiments demonstrate that P-element-induced site-specific male recombination is an efficient and general method to map Drosophila autosomal mutations.
Chen
B
B
Department of Pharmacology, Institute for Cell and Developmental Biology, University at Stony Brook, Stony Brook, New York 11794-8651, USA.
Chu
T
T
Harms
E
E
Gergen
J P
JP
Strickland
S
S
D000818:Animals; D002874:Chromosome Mapping; D004251:DNA Transposable Elements / Q000235:genetics; D004331:Drosophila melanogaster / Q000235:genetics*; D008297:Male; D008540:Meiosis / Q000235:genetics; D009154:Mutation; D011995:Recombination, Genetic
D004251:DNA Transposable Elements