Molecular weight of hyaluronate in the serum of patients with chronic liver disease.
Hyaluronate in tissue and lymph is known to be heterogenous and to show a wide range of molecular weights (10(4) to 10(7) Da). Serum hyaluronate concentrations are increased under various pathophysiological conditions such as liver disease, post-gastrectomy, and after the ingestion of food. To clarify whether the chromatographic patterns of hyaluronate in serum from patients with chronic liver disease are different under these conditions, we subjected sera to chromatography using a Sephacryl S 400 HR column. The chromatograms revealed that the hyaluronate in serum was eluted as a single peak at the position corresponding to the molecular weight of blue dextran, the molecular weight being more than 2 x 10(6) Da. The patterns of the chromatogram were similar among the patients with liver disease and the healthy subject although the heights of the peaks were different. Ingestion of food and a history of gastrectomy for gastric cancer did not influence the elution patterns of serum hyaluronate. These results indicate that hyaluronate in serum has molecular weight of more than 2 x 10(6) Da, and that its elution patterns are not influenced by pathophysiological factors, such as the severity of liver disease, or history of gastrectomy or by food intake in patients with chronic liver disease.
Murawaki
Y
Y
Second Department of Internal Medicine, Tottori University School of Medicine, Yonago, Japan.
Ikuta
Y
Y
Idobe
Y
Y
Koda
M
M
Kawasaki
H
H
D000328:Adult; D002850:Chromatography, Gel; D002908:Chronic Disease; D004435:Eating; D006526:Hepatitis C / Q000097:blood / Q000503:physiopathology; D006521:Hepatitis, Chronic / Q000097:blood / Q000503:physiopathology; D006801:Humans; D006820:Hyaluronic Acid / Q000097:blood*; D008103:Liver Cirrhosis / Q000097:blood / Q000503:physiopathology; D008107:Liver Diseases / Q000097:blood* / Q000503:physiopathology; D008297:Male; D008875:Middle Aged; D008970:Molecular Weight
D006820:Hyaluronic Acid