title: Increased apoptosis, changes in intracellular Ca2+, and functional alterations in lymphocytes and macrophages after in vitro exposure to static magnetic field.
abstract: Electromagnetic-related alteration of cellular functions is well documented for extremely low-frequency low-energy pulsing electromagnetic fields (ELF-EMF). In this study we examined the in vitro effects of static magnetic fields (SMF) on the cellular immune parameters of the C57BI/6 murine macrophages, spleen lymphocytes, and thymic cells. The cells were exposed in vitro for 24 h at 37 degrees C, 5% CO2, to 250-1500 G SMF. Exposure to the SMF resulted in the decreased phagocytic uptake of fluorescent latex microspheres, which was accompanied by an increased intracellular Ca2+ level in macrophages. Exposure to SMF decreased mitogenic responses in lymphocytes, as determined by incorporation of [3H]thymidine into the cells. This was associated with the increased Ca2+ influx in concanavalin A-stimulated lymphocytes. Furthermore, exposure to SMF produced markedly increased apoptosis of thymic cells, as determined by flow cytometry. Overall, in vitro exposure of immunocompetent cells to 250-1500 G SMF altered several functional parameters of C57BI/6 murine macrophages, thymocytes, and spleen lymphocytes.
authors.affiliation: TOXEN and Départment des Sciences Biologiques, Université du Québec à Montréal, Canada.
authors.forename: D
authors.initials: D
authors.lastname: Flipo
authors.forename: M
authors.initials: M
authors.lastname: Fournier
authors.forename: C
authors.initials: C
authors.lastname: Benquet
authors.forename: P
authors.initials: P
authors.lastname: Roux
authors.forename: C
authors.initials: C
authors.lastname: Le Boulaire
authors.forename: C
authors.initials: C
authors.lastname: Pinsky
authors.forename: F S
authors.initials: FS
authors.lastname: LaBella
authors.forename: K
authors.initials: K
authors.lastname: Krzystyniak
mesh_terms: D000818:Animals; D017209:Apoptosis; D002118:Calcium / Q000378:metabolism; D004574:Electromagnetic Fields / Q000009:adverse effects*; D066298:In Vitro Techniques; D007376:Interleukin-2; D007424:Intracellular Fluid; D008214:Lymphocytes / Q000502:physiology*; D008264:Macrophages / Q000502:physiology*; D051379:Mice; D010587:Phagocytosis / Q000187:drug effects
chemical_list: D007376:Interleukin-2; D002118:Calcium