Constitutive choline transport in Pseudomonas aeruginosa.
Pseudomonas aeruginosa has a choline uptake system which is expressed in bacteria grown in the presence of succinate and ammonium chloride as the carbon and nitrogen source, respectively. This system obeys Michaelis-Menten kinetics with an apparent Km value of 53 microM; its activity is not inhibited by high osmolarities in the medium but is partially inhibited by choline metabolites such as betaine and dimethylglycine.
Lucchesi
G I
GI
Departamento de Biología Molecular, Universidad Nacional de Río Cuarto, Córdoba, Argentina.
Pallotti
C
C
Lisa
A T
AT
Domenech
C E
CE
D000643:Ammonium Chloride / Q000494:pharmacology; D001622:Betaine / Q000494:pharmacology; D001692:Biological Transport; D002794:Choline / Q000378:metabolism* / Q000494:pharmacology; D007700:Kinetics; D009994:Osmolar Concentration; D011550:Pseudomonas aeruginosa / Q000378:metabolism*; D012521:Sarcosine / Q000031:analogs & derivatives / Q000494:pharmacology; D012965:Sodium Chloride / Q000494:pharmacology; D013386:Succinates / Q000494:pharmacology
D013386:Succinates; D000643:Ammonium Chloride; D001622:Betaine; D012965:Sodium Chloride; C025138:dimethylglycine; D002794:Choline; D012521:Sarcosine