Progenitor cell subsets and engraftment kinetics in children undergoing autologous peripheral blood stem cell transplantation.
The main objective of the present study was to determine the role of CD34+ cell subsets in the haemopoietic recovery of children undergoing peripheral blood stem cell transplantation. For this purpose, 38 leukaphereses from 33 children with malignancies mobilized with G-CSF were analysed. Using dual-colour flow cytometry, different subpopulations of CD34+ cells were quantified and the number of each reinfused subsets correlated with haemopoietic resurgence. Multivariate analysis showed that the number of CD34+CD38- cells and CD34+CD38+ cells correlated better with time to neutrophil and platelet recovery, respectively, than the total number of CD34+ cells. Threshold values for rapid haemopoietic recovery, determined by the receiver operating characteristic analysis, were found to be 0.5 X 10(6) CD34+CD38- cells for neutrophil engraftment, and 2.0x10(6) CD34+CD38+ cells for platelet recovery. It is suggested that the analysis of CD34+ cell subsets could increase understanding of the repopulation capacity of a given leukapheresis product in peripheral blood stem cell transplantation procedures in children. In particular, this procedure could be extremely useful when low numbers of CD34+ cells are collected.
Gonzalez-Requejo
A
A
Histocompatibility, Regional Transfusion Centre, Madrid, Spain.
Madero
L
L
Díaz
M A
MA
Villa
M
M
García-Escribano
C
C
Balas
A
A
Lillo
R
R
García-Sanchez
F
F
Benito
A
A
Vicario
J L
JL
D000293:Adolescent; D018952:Antigens, CD34 / Q000032:analysis*; D001792:Blood Platelets / Q000473:pathology; D002648:Child; D002675:Child, Preschool; D005260:Female; D005434:Flow Cytometry; D019337:Hematologic Neoplasms / Q000473:pathology / Q000628:therapy; D019650:Hematopoietic Stem Cell Mobilization; D018380:Hematopoietic Stem Cell Transplantation; D006412:Hematopoietic Stem Cells / Q000473:pathology*; D006801:Humans; D007937:Leukapheresis; D008297:Male; D009369:Neoplasms / Q000473:pathology / Q000628:therapy; D009504:Neutrophils / Q000473:pathology; D014182:Transplantation, Autologous
D018952:Antigens, CD34