Role of UEV-1A, a homologue of the tumor suppressor protein TSG101, in protection from DNA damage.
The open reading frame YGL087c in the budding yeast Saccharomyces cerevisiae genome encodes a polypeptide highly similar to the human UEV (ubiquitin-conjugating E2 enzyme variant) proteins, which have been proposed to belong to a family of putative dominant negative ubiquitin regulators. Deletion of the YGL087c open reading frame yields viable cells which are sensitive to UV irradiation or methyl methanesulfonate, but not to hydroxyurea. This phenotype is reminiscent of that of rad mutants and suggests that the YGL087c-encoded protein functions in a process related to tolerance to DNA damage. We also show that the mutant phenotype is fully complemented by expression of the human UEV-1A cDNA and we propose that UEV-1 proteins could also have a role in protecting higher eukaryotic cells from DNA damaging agents.
Thomson
T M
TM
Centre d'Investigació i Desenvolupament, C.S.I.C., Barcelona, Spain.
Khalid
H
H
Lozano
J J
JJ
Sancho
E
E
Ariño
J
J
D000595:Amino Acid Sequence; D004249:DNA Damage; D004268:DNA-Binding Proteins / Q000737:chemistry*; D056827:Endosomal Sorting Complexes Required for Transport; D016147:Genes, Tumor Suppressor; D006801:Humans; D008025:Ligases / Q000737:chemistry / Q000235:genetics / Q000502:physiology*; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D012441:Saccharomyces cerevisiae / Q000235:genetics; D017386:Sequence Homology, Amino Acid; D014157:Transcription Factors / Q000737:chemistry*; D044763:Ubiquitin-Conjugating Enzymes; D000099177:Tsg101 Protein
D004268:DNA-Binding Proteins; D056827:Endosomal Sorting Complexes Required for Transport; D008025:Ligases; D014157:Transcription Factors; D044763:Ubiquitin-Conjugating Enzymes; D000099177:Tsg101 Protein; C471624:UBE2V1 protein, human