Inhibition of establishment and growth of mouse liver metastases after treatment with interferon gamma and beta-1,3-D-glucan.
The purpose of this study was to investigate the combined antitumor effect of aminated beta-1,3-D-glucan (AG) and interferon-gamma (IFN-gamma) in an experimental liver metastasis model. Liver metastases were established by inoculation of C-26 colon carcinoma cells into the superior mesenteric vein of syngeneic mice. Treatment of mice started 24 hours after inoculation of tumor cells by daily intravenous injections of either AG, IFN-gamma, or a combination of both for a duration of 6 days. The resultant liver metastases were then quantified after an additional period of 11 days. Combination of IFN-gamma and AG inhibited the growth of liver metastases almost entirely. IFN-gamma was also very efficient, while AG alone did not exert any significant antitumor effect. These results, along with histological studies from mice receiving AG and IFN-gamma, indicated that activation and recruitment of liver macrophages may be a part of the mechanism responsible for the inhibition of metastatic growth observed in this study.
Sveinbjørnsson
B
B
Department of Experimental Pathology, Institute of Medical Biology, University of Tromsø, Norway.
Rushfeldt
C
C
Seljelid
R
R
Smedsrød
B
B
D000818:Animals; D000970:Antineoplastic Agents / Q000008:administration & dosage*; D002277:Carcinoma / Q000473:pathology*; D003110:Colonic Neoplasms / Q000473:pathology*; D004357:Drug Synergism; D005260:Female; D005936:Glucans / Q000494:pharmacology*; D007371:Interferon-gamma / Q000494:pharmacology* / Q000627:therapeutic use; D008113:Liver Neoplasms / Q000276:immunology / Q000473:pathology / Q000556:secondary*; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D009362:Neoplasm Metastasis / Q000517:prevention & control*; D011994:Recombinant Proteins; D047071:beta-Glucans
D000970:Antineoplastic Agents; D005936:Glucans; D011994:Recombinant Proteins; D047071:beta-Glucans; D007371:Interferon-gamma; C033363:beta-1,3-glucan