[Polymerase chain reaction in the detection of patients infected by Chlamydia trachomatis after treatment].
OBJECTIVE
To investigate the value of polymerase chain reaction (PCR) for follow-up patients infected by Chlamydia trachomatis.


METHODS
Follow-up specimens were collected from 30 patients. Chlamydia trachomatis positive were detected by PCR and direct fluorescence assay test (DFA) in the 30 patients before therapy. 15 patients were treated with minocycline (100 mg twice daily) for 10 days, and 15 patients were treated with 1.0 g of azithromycine as a single oral dose.


RESULTS
After 1-2 weeks of antimicrobial therapy, all patients had negative DFA for Chlamydia trachomatis, but 9 had positive Chlamydia trachomatis DNA as detected by PCR.


CONCLUSIONS
The 9 specimens were not confirmed to livae viable organisms of Chlamydia trachomatis. The debris of nonviable Chlamydia trachomatis DNA was excluded from urinogenital tract at about one month.
Wang
H
H
Department of Dermatology, Peking Union Medical College Hospital, Beijing.
Wang
J
J
Liu
Y
Y
D000328:Adult; D000900:Anti-Bacterial Agents / Q000627:therapeutic use*; D017963:Azithromycin / Q000627:therapeutic use; D002690:Chlamydia Infections / Q000188:drug therapy / Q000382:microbiology*; D002692:Chlamydia trachomatis / Q000302:isolation & purification*; D004269:DNA, Bacterial / Q000032:analysis; D005260:Female; D005500:Follow-Up Studies; D006801:Humans; D008297:Male; D008911:Minocycline / Q000627:therapeutic use*; D016133:Polymerase Chain Reaction
D000900:Anti-Bacterial Agents; D004269:DNA, Bacterial; D017963:Azithromycin; D008911:Minocycline