Gastric cytoprotective activity of dehydroleucodine in rats. Role of prostaglandins.
Previously, we reported that dehydroleucodine (DhL), a sesquiterpene lactone, protected the gastric mucosa of rats from absolute ethanol-induced lesions in a dose-dependent fashion. The mechanism is not mediated by an antiacid secretory action and DhL stimulated mucous production. In the present study, we report the effect of DhL on the mucosal production of prostaglandin E (PGE) and the mucosal release of PGE2 in rats stomach. DhL in acute treatment does not modify these values decreased by previous treatment with indomethacin or absolute ethanol. However, DhL in subchronic treatment significantly enhanced the mucosal production of PGE and the mucosal release of PGE2. Also, indomethacin pretreatment resulted in a significant reduction of the cytoprotective action of DhL. These results indicate the participation of endogenous prostaglandins in DhL protection against ethanol damage. Moreover, we suggest that the gastric protective activity of DhL against ethanol induced gastric mucosal damage is mediated, at least in part, through PGE and PGE2 in subchronic treatment.
Maria
A O
AO
Departamento de Farmacología, Universidad Nacional de San Luis, Chacabuco y Pedernera, San Luis, Argentina.
Franchi
A M
AM
Wendel
G H
GH
Gimeno
M
M
Guzman
J A
JA
Giordano
O S
OS
Guerreiro
E
E
D000818:Animals; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000037:antagonists & inhibitors / Q000633:toxicity; D000897:Anti-Ulcer Agents / Q000494:pharmacology*; D016718:Arachidonic Acid / Q000378:metabolism; D015232:Dinoprostone / Q000096:biosynthesis; D005260:Female; D005753:Gastric Mucosa / Q000166:cytology* / Q000187:drug effects; D007213:Indomethacin / Q000037:antagonists & inhibitors / Q000633:toxicity; D007783:Lactones / Q000494:pharmacology*; D008297:Male; D011458:Prostaglandins E / Q000096:biosynthesis / Q000502:physiology*; D011863:Radioimmunoassay; D051381:Rats; D017208:Rats, Wistar; D012717:Sesquiterpenes / Q000494:pharmacology*
D000894:Anti-Inflammatory Agents, Non-Steroidal; D000897:Anti-Ulcer Agents; D007783:Lactones; D011458:Prostaglandins E; D012717:Sesquiterpenes; D016718:Arachidonic Acid; C079949:dehydroleucodine; D015232:Dinoprostone; D007213:Indomethacin