Liquid chromatographic separation of hexopyranosylated cytosine nucleosides from their degradation products.
Development of a liquid chromatographic method which can separate each of a series of hexopyranosylated cytosine nucleosides from their degradation products formed at acid, neutral and basic pH is described. Both silica-based reverse-phase and polymer columns were examined. Influence of the mobile phase pH, ion-pairing agent, concentration of the buffer and type and concentration of organic modifier were systematically investigated. The concentration of the ion-pairing agent and the buffer were found to have a major effect on selectivity. Samples were finally analyzed on a poly(styrene-divinylbenzene), PLRP-S 100 A (8 microns) 250 x 4.6 mm I.D. column at 60 degrees C and with a mobile phase consisting of acetonitrile-sodium octanesulphonate (pH 2.5; 0.02 M)-potassium phosphate buffer (pH 2.5; 0.2 M)-water (X:25:50:25-X, v/v, where X is variable).
Thoithi
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G
Katholieke Universiteit Leuven, Laboratorium voor Farmaceutische Chemie en Analyse van Geneesmiddelen, Faculteit Farmaceutische Wetenschappen, Belgium.
Van Schepdael
A
A
Herdewijn
P
P
Roets
E
E
Hoogmartens
J
J
D000970:Antineoplastic Agents / Q000737:chemistry / Q000302:isolation & purification*; D000998:Antiviral Agents / Q000737:chemistry / Q000302:isolation & purification*; D002853:Chromatography, Liquid; D003596:Cytosine / Q000031:analogs & derivatives / Q000737:chemistry / Q000302:isolation & purification*; D006863:Hydrogen-Ion Concentration; D007202:Indicators and Reagents; D011741:Pyrimidine Nucleosides / Q000737:chemistry / Q000302:isolation & purification*; D012044:Regression Analysis
D000970:Antineoplastic Agents; D000998:Antiviral Agents; D007202:Indicators and Reagents; D011741:Pyrimidine Nucleosides; D003596:Cytosine