Influence of maturation and ageing on the biotransformation of noradrenaline in the rat.
The present investigation was undertaken to study the influence of maturation and ageing on the disposition of noradrenaline by the aorta, heart (ventricle), liver and kidney of the rat. Slices of these tissues taken from rats aged less than 18 h, 2.5-3 months or 18-24 months were incubated with 0.1 mumol.l-1 3H-amine during 30 min. At the end of this period, the accumulation of the intact amine in the tissue, as well as the 3H-metabolites formed (3,4-dihydroxyphenylethylglycol, 3,4-dihydroxymandelic acid, normetanephrine and O-methylated deaminated metabolites) were determined by scintillation counting. The results obtained show that in the rat: 1) at any age, noradrenaline is preferentially deaminated; 2) while the capacity of the sympathetic nerve terminals in accumulating noradrenaline is rather well developed at birth, the metabolic system for its degradation is still immature; 3) aldehyde dehydrogenase activity or that of its co-factor (or both) of the heart is apparently missing at birth; 4) removal of noradrenaline by the liver and the kidney did not change with ageing, while that by the aorta decreased and that by the heart increased.
Guimarães
J T
JT
Institute of Pharmacology and Therapeutics, Faculty of Medicine, Porto, Portugal.
Paiva
M Q
MQ
Moura
D
D
Guimarães
S
S
D000375:Aging / Q000378:metabolism*; D000818:Animals; D000831:Animals, Newborn; D001011:Aorta / Q000254:growth & development / Q000378:metabolism*; D001711:Biotransformation; D005260:Female; D006321:Heart / Q000254:growth & development; D007668:Kidney / Q000254:growth & development / Q000378:metabolism*; D008099:Liver / Q000254:growth & development / Q000378:metabolism*; D008297:Male; D008333:Mandelic Acids / Q000378:metabolism; D008734:Methoxyhydroxyphenylglycol / Q000031:analogs & derivatives / Q000378:metabolism; D009206:Myocardium / Q000378:metabolism*; D009284:Naphthols / Q000378:metabolism; D009638:Norepinephrine / Q000378:metabolism*; D009647:Normetanephrine / Q000378:metabolism; D011409:Propylene Glycols / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar
D008333:Mandelic Acids; D009284:Naphthols; D011409:Propylene Glycols; D009647:Normetanephrine; C056310:1-((3-hydroxy-4-methoxy)-1-naphthoxy)-2,3-propanediol; D008734:Methoxyhydroxyphenylglycol; C000989:3,4-dihydroxymandelic acid; C010117:3,4-dihydroxyphenylglycol; D009638:Norepinephrine