Trophic effect of porcine Sertoli cells on rat and human ventral mesencephalic cells and hNT neurons in vitro.
The poor survival of embryonic dopaminergic (DA) neurons transplanted into patients with Parkinson's disease (PD) has encouraged researchers to search for new methods to affect the short- as well as long-term survival of these neurons after transplantation. In several previous rodent studies Sertoli cells increased survival of islet cells and chromaffin cells when cotransplanted in vivo. The aims of this study were to investigate whether porcine Sertoli cells had a positive effect on the survival and maturation of rat and human DA neurons, and whether the Sertoli cells had an effect on differentiation of neurons derived from a human teratocarcinoma cell line (hNT neurons). A significant increase of tyrosine hydroxylase (TH)-positive neurons of both rat and human ventral mesencephalic tissue was found when cocultured with Sertoli cells. Furthermore, there was a significantly increased soma size and neurite outgrowth of neurons in the coculture treated group. The Sertoli cell and hNT coculture also revealed an increased number of TH-positive cells. These results demonstrate that the wide variety of proteins and factors secreted by porcine Sertoli cells benefit the survival and maturation of embryonic DA neurons and suggest that cotransplantation of Sertoli cells and embryonic DA neurons may be useful for a cell transplantation therapy in PD.
Othberg
A I
AI
Department of Surgery, University of South Florida, College of Medicine, Tampa 33612, USA.
Willing
A E
AE
Cameron
D F
DF
Anton
A
A
Saporta
S
S
Freeman
T B
TB
Sanberg
P R
PR
D000818:Animals; D016380:Brain Tissue Transplantation / Q000473:pathology* / Q000502:physiology; D002452:Cell Count; D002454:Cell Differentiation; D002470:Cell Survival; D018920:Coculture Techniques; D016332:Fetal Tissue Transplantation / Q000473:pathology / Q000502:physiology; D006801:Humans; D008297:Male; D008636:Mesencephalon / Q000166:cytology / Q000201:enzymology / Q000637:transplantation*; D009474:Neurons / Q000201:enzymology / Q000473:pathology*; D010300:Parkinson Disease / Q000473:pathology / Q000601:surgery; D051381:Rats; D012708:Sertoli Cells / Q000502:physiology*; D013552:Swine; D014407:Tumor Cells, Cultured; D014446:Tyrosine 3-Monooxygenase / Q000378:metabolism
D014446:Tyrosine 3-Monooxygenase