Selective intraarterial gene delivery into a canine meningioma.
OBJECTIVE
The goal of this study was to evaluate gene delivery to a benign brain tumor.


METHODS
A recombinant adenovirus vector bearing the Escherichia coli beta-galactosidase reporter gene was selectively injected into the vascular supply of a spontaneously occurring canine olfactory groove meningioma. The tumor and a small amount of peritumoral brain tissue were removed 5 days after viral injection and stained with X-Gal to assess gene delivery. The authors noted significant beta-galactosidase gene expression by the tumor, but not by surrounding brain tissue. No obvious viral-related cytotoxicity was noted.


CONCLUSIONS
The authors found that meningiomas can be successfully transduced by adenovirus vectors by using endovascular techniques.
Chauvet
A E
AE
Department of Medical Sciences, School of Veterinary Medicine, University of Wisconsin, Madison, USA.
Kesava
P P
PP
Goh
C S
CS
Badie
B
B
D000256:Adenoviridae / Q000235:genetics; D000818:Animals; D004396:Coloring Agents; D004274:DNA, Recombinant / Q000235:genetics; D004285:Dogs; D004926:Escherichia coli / Q000235:genetics; D005004:Ethmoid Bone / Q000473:pathology; D005260:Female; D005697:Galactosides; D015971:Gene Expression Regulation, Enzymologic; D015972:Gene Expression Regulation, Neoplastic; D017930:Genes, Reporter / Q000235:genetics; D015316:Genetic Therapy; D005822:Genetic Vectors; D007211:Indoles; D007269:Injections, Intra-Arterial; D008577:Meningeal Neoplasms / Q000098:blood supply / Q000473:pathology / Q000601:surgery / Q000628:therapy / Q000662:veterinary*; D008579:Meningioma / Q000098:blood supply / Q000473:pathology / Q000601:surgery / Q000628:therapy / Q000662:veterinary*; D014161:Transduction, Genetic; D001616:beta-Galactosidase / Q000235:genetics
D004396:Coloring Agents; D004274:DNA, Recombinant; D005697:Galactosides; D007211:Indoles; D001616:beta-Galactosidase; C044888:5-bromo-4-chloro-3-indolyl beta-galactoside