title: A poxvirus-encoded semaphorin induces cytokine production from monocytes and binds to a novel cellular semaphorin receptor, VESPR.
abstract: The vaccinia virus A39R protein is a member of the semaphorin family. A39R.Fc protein was used to affinity purify an A39R receptor from a human B cell line. Tandem mass spectrometry of receptor peptides yielded partial amino acid sequences that allowed the identification of corresponding cDNA clones. Sequence analysis of this receptor indicated that it is a novel member of the plexin family and identified a semaphorin-like domain within this family, thus suggesting an evolutionary relationship between receptor and ligand. A39R up-regulated ICAM-1 on, and induced cytokine production from, human monocytes. These data, then, describe a receptor for an immunologically active semaphorin and suggest that it may serve as a prototype for other plexin-semaphorin binding pairs.
authors.affiliation: Department of Molecular Biology, Immunex Corporation, Seattle, Washington 98101, USA.
authors.forename: M R
authors.initials: MR
authors.lastname: Comeau
authors.forename: R
authors.initials: R
authors.lastname: Johnson
authors.forename: R F
authors.initials: RF
authors.lastname: DuBose
authors.forename: M
authors.initials: M
authors.lastname: Petersen
authors.forename: P
authors.initials: P
authors.lastname: Gearing
authors.forename: T
authors.initials: T
authors.lastname: VandenBos
authors.forename: L
authors.initials: L
authors.lastname: Park
authors.forename: T
authors.initials: T
authors.lastname: Farrah
authors.forename: R M
authors.initials: RM
authors.lastname: Buller
authors.forename: J I
authors.initials: JI
authors.lastname: Cohen
authors.forename: L D
authors.initials: LD
authors.lastname: Strockbine
authors.forename: C
authors.initials: C
authors.lastname: Rauch
authors.forename: M K
authors.initials: MK
authors.lastname: Spriggs
mesh_terms: D000595:Amino Acid Sequence; D001402:B-Lymphocytes / Q000378:metabolism; D015815:Cell Adhesion Molecules / Q000235:genetics; D002460:Cell Line; D003001:Cloning, Molecular; D016207:Cytokines / Q000096:biosynthesis*; D019143:Evolution, Molecular; D006801:Humans; D018799:Intercellular Adhesion Molecule-1 / Q000378:metabolism; D008024:Ligands; D008565:Membrane Proteins / Q000737:chemistry / Q000235:genetics / Q000502:physiology*; D008969:Molecular Sequence Data; D009000:Monocytes / Q000276:immunology* / Q000378:metabolism; D009419:Nerve Tissue Proteins / Q000235:genetics; D011956:Receptors, Cell Surface / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D011991:Receptors, Virus; D011994:Recombinant Proteins / Q000235:genetics / Q000276:immunology / Q000378:metabolism; D017386:Sequence Homology, Amino Acid; D014018:Tissue Distribution; D014616:Vaccinia virus / Q000235:genetics / Q000276:immunology*; D014764:Viral Proteins / Q000235:genetics / Q000276:immunology* / Q000378:metabolism*
chemical_list: D015815:Cell Adhesion Molecules; D016207:Cytokines; D008024:Ligands; D008565:Membrane Proteins; D009419:Nerve Tissue Proteins; D011956:Receptors, Cell Surface; D011991:Receptors, Virus; D011994:Recombinant Proteins; C112038:VESPR semaphorin receptor; D014764:Viral Proteins; C091529:plexin; D018799:Intercellular Adhesion Molecule-1