Establishment and characterization of a human glioblastoma multiforme cell line.
Cell lines provide a useful system for further understanding the biology of glioblastoma multiforme. In this study, a new glioblastoma multiforme cell line, GATAGM-96 (Gulhane Askeri Tip Akademisi-Gliblastoma Multiforme-96), was established from a tumor specimen removed from an 80-year-old male patient who underwent surgery for intracranial tumor. Morphologic examination, immunocytochemical staining, growth kinetics, and karyotypic characteristics of this cell line were studied. The cytoskeleton was positive for neuron-specific enolase, vimentin, and neurofilament, and it was negative for glial fibrillary acidic protein, S-100 protein, p53 protein, epidermal growth factor, and transforming growth factor alpha. Growth kinetic studies demonstrated an approximate population doubling time of 38 to 42 h and a colony forming efficiency of 83.3%. The karyotype of the cells demonstrated it as hyperdiploid, with a large subpopulation of polyploid cells. There were numerous structural and numerical chromosome aberrations; most of them were present as clonal events. The phenotypic and chromosomal features detailed on the GATAGM-96 cell line should make it a useful addition to the cell lines currently available for in vitro and in vivo studies of glioblastoma multiforme.
Bakir
A
A
Department of Neurosurgery, Gulhane School of Medicine, Ankara, Turkey.
Gezen
F
F
Yildiz
O
O
Ayhan
A
A
Kahraman
S
S
Kruse
C A
CA
Varella-Garcia
M
M
Yildiz
F
F
Kubar
A
A
D000368:Aged; D000369:Aged, 80 and over; D018929:Cell Culture Techniques / Q000379:methods*; D002455:Cell Division; D005909:Glioblastoma / Q000737:chemistry / Q000235:genetics / Q000473:pathology* / Q000648:ultrastructure; D006801:Humans; D007150:Immunohistochemistry; D007621:Karyotyping; D008297:Male; D014407:Tumor Cells, Cultured; D014410:Tumor Stem Cell Assay