Characterization of DNA fragments that show anomalously rapid migration in non-denaturing polyacrylamide gels.
Several DNA fragments that show anomalously rapid migration in nondenaturing polyacrylamide gels have been cloned from digests of bovine genomic DNA. Electrophoretic behaviors of these fragments were analyzed in detail under various conditions. The results indicated that these fragments may adopt non-B DNA conformation.
Tsujibayashi
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Department of Biology, Faculty of Science, Konan University, Kobe, Japan.
Tagashira
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Ohyama
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D000818:Animals; D002417:Cattle; D003001:Cloning, Molecular; D004247:DNA / Q000737:chemistry*; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods; D016678:Genome; D007202:Indicators and Reagents; D009690:Nucleic Acid Conformation; D009838:Oligodeoxyribonucleotides / Q000737:chemistry* / Q000302:isolation & purification
D007202:Indicators and Reagents; D009838:Oligodeoxyribonucleotides; D004247:DNA