title: Immunohistochemical study of gamma-aminobutyric acid and bombesin/gastrin releasing peptide in human dental pulp.
abstract: This study investigated the presence of the putative peripheral neuromodulators Gamma-aminobutyric Acid (GABA) and Bombesin/Gastrin-Releasing Peptide (BN/GRP) in the human tooth pulp. Caries free and asymptomatic carious teeth were processed for paraffin embedding and sectioned at six microns. From each specimen, sections were stained with hematoxylin and eosin; other sections were subjected to Avidin-Biotin-Peroxidase Complex immunohistochemistry for GABA and BN/GRP. Sections of rat brain and small cell lung carcinoma served as positive controls. Results indicate the presence of specific GABA-like and BN/GRP-like immunoreactivity within the pulps of both normal and carious teeth. Overall staining for both ligands was significantly more intense within inflamed pulps. Based on their actions elsewhere, GABA and BN/GRP may play a role in the dental pulp as peripheral neuromodulators or as growth factors.
authors.affiliation: Indiana University School of Dentistry, Indianapolis 46202-5186, USA.
authors.forename: W M
authors.initials: WM
authors.lastname: Todd
authors.forename: A H
authors.initials: AH
authors.lastname: Kafrawy
authors.forename: C W
authors.initials: CW
authors.lastname: Newton
authors.forename: C E
authors.initials: CE
authors.lastname: Brown
mesh_terms: D000818:Animals; D001839:Bombesin / Q000032:analysis* / Q000378:metabolism; D003731:Dental Caries / Q000378:metabolism*; D003782:Dental Pulp / Q000737:chemistry* / Q000378:metabolism; D019886:Gastrin-Releasing Peptide / Q000032:analysis*; D006801:Humans; D007124:Immunoenzyme Techniques; D015213:Neuroimmunomodulation; D009479:Neuropeptides / Q000032:analysis*; D009804:Odontoblasts / Q000378:metabolism; D051381:Rats; D018709:Statistics, Nonparametric; D005680:gamma-Aminobutyric Acid / Q000032:analysis*
chemical_list: D009479:Neuropeptides; D005680:gamma-Aminobutyric Acid; D019886:Gastrin-Releasing Peptide; D001839:Bombesin