2-Ketocyclohexanecarboxyl coenzyme A hydrolase, the ring cleavage enzyme required for anaerobic benzoate degradation by Rhodopseudomonas palustris.
2-Ketocyclohexanecarboxyl coenzyme A (2-ketochc-CoA) hydrolase has been proposed to catalyze an unusual hydrolytic ring cleavage reaction as the last unique step in the pathway of anaerobic benzoate degradation by bacteria. This enzyme was purified from the phototrophic bacterium Rhodopseudomonas palustris by sequential Q-Sepharose, phenyl-Sepharose, gel filtration, and hydroxyapatite chromatography. The sequence of the 25 N-terminal amino acids of the purified hydrolase was identical to the deduced amino acid sequence of the badI gene, which is located in a cluster of genes involved in anaerobic degradation of aromatic acids. The deduced amino acid sequence of badI indicates that 2-ketochc-CoA hydrolase is a member of the crotonase superfamily of proteins. Purified BadI had a molecular mass of 35 kDa as determined by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and a native molecular mass of 134 kDa as determined by gel filtration. This indicates that the native form of the enzyme is a homotetramer. The purified enzyme was insensitive to oxygen and catalyzed the hydration of 2-ketochc-CoA to yield pimelyl-CoA with a specific activity of 9.7 micromol min(-1) mg of protein(-1). Immunoblot analysis using polyclonal antiserum raised against the purified hydrolase showed that the synthesis of BadI is induced by growth on benzoate and other proposed benzoate pathway intermediates but not by growth on pimelate or succinate. An R. palustris mutant, carrying a chromosomal disruption of badI, did not grow with benzoate and other proposed benzoate pathway intermediates but had wild-type doubling times on pimelate and succinate. These data demonstrate that BadI, the 2-ketochc-CoA hydrolase, is essential for anaerobic benzoate metabolism by R. palustris.
Pelletier
D A
DA
Department of Microbiology and Center for Biocatalysis and Bioprocessing, The University of Iowa, Iowa City 52242, USA.
Harwood
C S
CS
D000214:Acyl Coenzyme A / Q000378:metabolism; D000595:Amino Acid Sequence; D000693:Anaerobiosis; D001426:Bacterial Proteins / Q000235:genetics / Q000302:isolation & purification; D001565:Benzoates / Q000378:metabolism*; D001711:Biotransformation; D003001:Cloning, Molecular; D004746:Enoyl-CoA Hydratase / Q000235:genetics; D008969:Molecular Sequence Data; D005810:Multigene Family; D015183:Restriction Mapping; D012241:Rhodopseudomonas / Q000201:enzymology* / Q000235:genetics / Q000254:growth & development; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D013869:Thiolester Hydrolases / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*
C112170:2-ketocyclohexanecarboxyl-coenzyme A; D000214:Acyl Coenzyme A; D001426:Bacterial Proteins; D001565:Benzoates; C112169:pimelyl-coenzyme A; C106551:2-ketocyclohexanecarboxyl-coenzyme A hydrolase; D013869:Thiolester Hydrolases; D004746:Enoyl-CoA Hydratase