title: Quercetin is recovered in human plasma as conjugated derivatives which retain antioxidant properties.
abstract: Quercetin is one of the most abundant flavonoids in the human diet. This study aimed to determine the plasma concentrations of quercetin in 10 healthy volunteers after the consumption of a complex meal rich in plant products. Quercetin was determined in plasma (2 h before, and 3, 7 and 20 h after the meal), and in a duplicated portion of the meal by HPLC analysis with an electrochemical detection. The amount of ingested quercetin was estimated to be 87 mg. Before the meal, quercetin concentration in hydrolyzed plasmas ranged from 28 to 142 nM. A marked increase was observed 3 h after the meal in all subjects, with a mean concentration of 373 nM (S.E.M. = 61). After 7 h, quercetin concentration in hydrolyzed plasmas decreased and after 20 h basal levels were found again. The antioxidant capacities of quercetin, 3'-O-methylquercetin, and of some of their conjugated derivatives were compared by the measurement of the conjugated dienes resulting from the Cu2+-induced oxidation of human LDL. 3'-O-Methylquercetin and conjugated derivatives of quercetin significantly prolonged the lag phase, but the magnitude of their effect was about half that of the aglycone.
authors.affiliation: Unité des Maladies Métaboliques et Micronutriments, I.N.R.A. de Clermont-Ferrand/Theix, St Genès-Champanelle, France. manach@clermont.inra.fr
authors.forename: C
authors.initials: C
authors.lastname: Manach
authors.forename: C
authors.initials: C
authors.lastname: Morand
authors.forename: V
authors.initials: V
authors.lastname: Crespy
authors.forename: C
authors.initials: C
authors.lastname: Demigné
authors.forename: O
authors.initials: O
authors.lastname: Texier
authors.forename: F
authors.initials: F
authors.lastname: Régérat
authors.forename: C
authors.initials: C
authors.lastname: Rémésy
mesh_terms: D000284:Administration, Oral; D000328:Adult; D000975:Antioxidants / Q000008:administration & dosage* / Q000378:metabolism*; D002851:Chromatography, High Pressure Liquid; D005260:Female; D005966:Glucuronidase / Q000097:blood; D006801:Humans; D006868:Hydrolysis; D008297:Male; D008875:Middle Aged; D010084:Oxidation-Reduction / Q000187:drug effects; D011794:Quercetin / Q000008:administration & dosage* / Q000097:blood*; D013429:Sulfatases / Q000097:blood; D014675:Vegetables / Q000737:chemistry; D014920:Wine / Q000032:analysis
chemical_list: D000975:Antioxidants; D011794:Quercetin; D013429:Sulfatases; D005966:Glucuronidase