The genomic structure of the chicken ICSBP gene and its transcriptional regulation by chicken interferon.
The chicken interferon consensus sequence binding protein (ChICSBP) gene spans over 9 kb of DNA and consists, as its murine homolog, of nine exons. The first untranslated exon was identified by 5'-RACE technology. The second exon contains the translation initiation codon. Canonical consensus splice sites are found on every exon/intron junction. The introns are generally smaller than their mammalian counterparts. The ChICSBP and ChIRF-1 genes have been mapped by fluorescence in situ hybridization to different microchromosomes. The transcription start site has been mapped by primer extension. Inspection of the DNA sequence of a genomic clone containing the first exon and the region 1700-bp upstream revealed several potential cisregulatory elements of transcription. The ChICSBP mRNA is induced by recombinant ChIFN type I and ChIFN-gamma. A palindromic IFN regulatory element (pIRE) with high sequence homology to gamma activation site (GAS) sequences was functionally required in transient transfection assays for the induction of transcription by ChIFN-gamma.
Dosch
E
E
Institute for Virology and Immunobiology, University of Würzburg, Germany.
Zöller
B
B
Redmann-Müller
I
I
Nanda
I
I
Schmid
M
M
Viciano-Gofferge
A
A
Jungwirth
C
C
D000818:Animals; D001483:Base Sequence; D002645:Chickens; D002874:Chromosome Mapping; D003001:Cloning, Molecular; D016384:Consensus Sequence; D018076:DNA, Complementary; D050835:Interferon Regulatory Factors; D007372:Interferons / Q000378:metabolism*; D008969:Molecular Sequence Data; D011401:Promoter Regions, Genetic; D011505:Protein-Tyrosine Kinases / Q000235:genetics; D012097:Repressor Proteins / Q000235:genetics*; D018139:Simplexvirus / Q000201:enzymology; D014158:Transcription, Genetic; D000099185:Interferon Regulatory Factor-8
D018076:DNA, Complementary; D050835:Interferon Regulatory Factors; D007372:Interferons; D011505:Protein-Tyrosine Kinases; D012097:Repressor Proteins; D000099185:Interferon Regulatory Factor-8