In vivo effect of interleukin-1 alpha on colony-stimulating factor-1 gene expression in the dental follicle of the rat molar.
Interleukin-1 alpha (IL-1 alpha) acts in vitro to enhance the gene expression of colony-stimulating factor-1 (CSF-1) of dental follicle cells. Because the dental follicle is required for tooth eruption and because CSF-1 appears to be a key molecule in initiating eruption, it was the aim of this study to determine if IL-1 alpha could enhance the expression of the CSF-1 gene in the dental follicle in vivo. To determine this, rats were injected with IL-1 alpha at different ages postnatally and the total RNA was isolated from the dental follicles of the first mandibular molars. Reverse transcription-polymerase chain reaction showed that IL-1 alpha enhanced the expression of CSF-1 in the follicle. Thus, IL-1 alpha may play a part in the cascade of molecular signals that initiate eruption by its effect upon the CSF-1 gene.
Wise
G E
GE
Department of Veterinary Anatomy and Cell Biology, School of Veterinary Medicine, Louisiana State University, Baton Rouge 70803-8408, USA.
D000818:Animals; D003795:Dental Sac / Q000378:metabolism*; D005786:Gene Expression Regulation; D007375:Interleukin-1 / Q000494:pharmacology*; D016173:Macrophage Colony-Stimulating Factor / Q000032:analysis / Q000235:genetics*; D008334:Mandible; D008963:Molar; D016133:Polymerase Chain Reaction; D012313:RNA / Q000032:analysis / Q000235:genetics; D051381:Rats; D015398:Signal Transduction / Q000235:genetics; D014078:Tooth Eruption / Q000235:genetics; D014158:Transcription, Genetic
D007375:Interleukin-1; D012313:RNA; D016173:Macrophage Colony-Stimulating Factor