title: Analysis of ACE inhibitors in pharmaceutical dosage forms by derivative UV spectroscopy and liquid chromatography (HPLC).
abstract: Derivative UV spectroscopy and high performance liquid chromatography (HPLC) were applied to the determination of angiotensin-converting enzyme (ACE) inhibitors in their pharmaceutical dosage forms. For spectrophotometric determinations, the more appropriate derivative order was selected for each drug: ramipril (third-order), benazepril (second-order), enalapril maleate (second-order), lisinopril (first- and second-order) and quinapril (first-order). Reverse phase HPLC procedures (ODS column) were developed able to provide a single, symmetric peak for each drug; mixtures A-B, where A is 20 mM sodium heptansulphonate (pH 2.5) and B is acetonitrile-THF (95:5 v/v), proved to be suitable mobile phases to obtain selective separations of the cited ACE inhibitors. At ambient temperature, a low pH value (2.5) was found to be critical to avoid peak splitting and band broadening.
authors.affiliation: Dipartimento di Scienze Farmaceutiche, Bologna, Italy.
authors.forename: D
authors.initials: D
authors.lastname: Bonazzi
authors.forename: R
authors.initials: R
authors.lastname: Gotti
authors.forename: V
authors.initials: V
authors.lastname: Andrisano
authors.forename: V
authors.initials: V
authors.lastname: Cavrini
mesh_terms: D000806:Angiotensin-Converting Enzyme Inhibitors / Q000032:analysis*; D002138:Calibration; D002214:Capsules; D002851:Chromatography, High Pressure Liquid; D006863:Hydrogen-Ion Concentration; D013056:Spectrophotometry, Ultraviolet; D013607:Tablets
chemical_list: D000806:Angiotensin-Converting Enzyme Inhibitors; D002214:Capsules; D013607:Tablets