[Experimental study on homoiothermic extracorporeal liver preservation].
To prove that perfused liver can be preserved at room temperature (22 degrees C), we made the experiment, in which HTK was basic solution, perfluorocarbon acted as oxygen carrier and lipid acted as energy substrate in the homoiothermy condition, pig liver organs were perfused extracorporeally through the V. portal with perfusion solution. In fixed period of time the perfusion solution from the liver was taken and analysed to determine for liver biochemical function and observe the concentration and size of hepatocellular mitochondria. In oxygen carrier perfusion solution the ammonia concentration was low, urea concentration was high, damage to mitochondria was minimum and by addition of lipid emulsion the concentration of glucose can be maintained. The experimental and control data were obviously significant. The result demonstrated that oxygen carrier (perfluorocarbon) as oxygen supplier can provides enough oxygen to liver cell, at the sametime, lipid emulsion intralipid) provides energy so as the perfused liver can be preserved at high temperature, i.e. 22 degrees C at relative long time (40 hours) and still has the function of removing toxic substance such as ammonia and converting it to urea, meanwhile maintain the normal structure of mitochondria. The preservation of perfused liver at homoiothermy is possible.
Qiu
H
H
Hospital of Hubei Medical University, Wuhan.
Chen
Y
Y
Gruenert
A
A
D000818:Animals; D005217:Fat Emulsions, Intravenous; D005260:Female; D005466:Fluorocarbons; D008099:Liver; D008297:Male; D009926:Organ Preservation; D019188:Organ Preservation Solutions; D013552:Swine; D013696:Temperature
D005217:Fat Emulsions, Intravenous; D005466:Fluorocarbons; D019188:Organ Preservation Solutions