Corticosterone attenuates zinc-induced neurotoxicity in primary hippocampal cultures.
Primary hippocampal cultures derived from newborn rats were exposed to zinc chloride at 50, 75, 100, 150 and 200 microM concentrations. Neuronal injury was assessed morphologically and by the lactate dehydrogenase (LDH) efflux assay. Zinc exposure increased LDH efflux in a concentration-dependent manner. Exposure to 100 microM zinc for 24 h resulted in beading of neurites and swelling of neuronal soma. When cultures were co-exposed to zinc at 100 microM and corticosterone in the range of 10-5 to 10-7 M, degeneration of neurons caused by zinc was attenuated. Our study suggests that corticosterone can protect neurons from zinc-induced neurotoxicity at low doses.
Sunanda
Department of Neurophysiology, National Institute of Mental Health and Neuro Sciences, Bangalore 560 029, India.
Rao
B S
BS
Raju
T R
TR
D000704:Analysis of Variance; D000818:Animals; D000831:Animals, Newborn; D002478:Cells, Cultured; D003345:Corticosterone / Q000494:pharmacology*; D006624:Hippocampus / Q000166:cytology / Q000187:drug effects* / Q000201:enzymology; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D009474:Neurons / Q000187:drug effects* / Q000201:enzymology; D018696:Neuroprotective Agents / Q000494:pharmacology*; D009498:Neurotoxins / Q000037:antagonists & inhibitors*; D051381:Rats; D017208:Rats, Wistar; D015032:Zinc / Q000037:antagonists & inhibitors*
D018696:Neuroprotective Agents; D009498:Neurotoxins; D007770:L-Lactate Dehydrogenase; D015032:Zinc; D003345:Corticosterone