title: Sequential CD34+ and CD4+ cell selection from leukapheresis components.
abstract: We tested the feasibility of sequential selection of CD34+ and CD4+ cell-enriched fractions from aliquots of five autologous leukapheresis components. CD34+ cells were selected from a median 8.0 x 10(8) mononuclear cells using the CellPro CEPRATE LC cell separation system. All cells in the CD34-depleted fraction (median 4.8 x 10(8), range 0.6-10.0 x 10(8) were then incubated with the appropriate antibodies for CD4+ cell selection and passed through a second LC column. Median target cell purities of the CD34+ cell-enriched fraction and CD4+ cell-enriched fraction were 90.5% and 86.0%, respectively. This study demonstrates that high purity CD34+ and CD4+ cell-enriched fractions can be isolated sequentially from leukapheresis components. In addition, CD8+ lymphocytes, implicated in graft-versus-host disease, were depleted in the course of both positive selection procedures. This approach could decrease the number of donor procedures by providing separate CD34(+)-enriched and CD4(+)-enriched populations for allogeneic peripheral blood progenitor cell transplantation and subsequent donor lymphocyte infusion from the same leukapheresis component.
authors.affiliation: Cell Manipulation and Gene Transfer Laboratories, Harvard Medical School, Boston, MA 02115, USA.
authors.forename: I J
authors.initials: IJ
authors.lastname: Webb
authors.forename: M
authors.initials: M
authors.lastname: Beach
authors.forename: J F
authors.initials: JF
authors.lastname: Daley
authors.forename: D M
authors.initials: DM
authors.lastname: Schott
authors.forename: K C
authors.initials: KC
authors.lastname: Anderson
mesh_terms: D018952:Antigens, CD34; D015496:CD4-Positive T-Lymphocytes; D002469:Cell Separation; D006801:Humans; D007937:Leukapheresis; D008212:Lymphocyte Depletion / Q000379:methods*
chemical_list: D018952:Antigens, CD34