Monoclonal antibodies putatively identifying porcine B cells.
Comparison was made of the binding of 38 test and three standard monoclonal antibodies (mAbs) to B cells from various pig lymphoid tissues by flow cytometry (FCM) and immunohistochemistry. Some mAbs were also tested on B cells from foetal pig tissues. Twenty of the new mAbs bound, though to variable degrees, to porcine B cells but only three were given cluster assignations: C35 (#147) and BB6-11C9 (#167) were assigned to wCD21 and 2F6/8 (#057) was assigned to SWC7.
Denham
S
S
BBSRC Institute for Animal Health, Pirbright Laboratory, Woking, UK.
Zwart
R J
RJ
Whittall
J T
JT
Pampusch
M
M
Corteyn
A H
AH
Bianchi
A T
AT
Murtaugh
M P
MP
Parkhouse
R M
RM
Tlaskalova
H
H
Sinkora
J
J
Sinkora
M
M
Rehakova
Z
Z
D000818:Animals; D000911:Antibodies, Monoclonal / Q000378:metabolism*; D000937:Antigen-Antibody Reactions; D015703:Antigens, CD / Q000276:immunology; D016175:B-Lymphocyte Subsets / Q000276:immunology*; D001666:Binding Sites, Antibody; D005312:Fetal Blood / Q000166:cytology / Q000276:immunology; D005333:Fetus; D005434:Flow Cytometry / Q000592:standards / Q000662:veterinary; D007136:Immunoglobulins / Q000378:metabolism; D010581:Peyer's Patches / Q000276:immunology / Q000378:metabolism; D012015:Reference Standards; D013154:Spleen / Q000166:cytology / Q000276:immunology; D013552:Swine / Q000276:immunology*
D000911:Antibodies, Monoclonal; D015703:Antigens, CD; D007136:Immunoglobulins