title: Secretory immunoglobulin A in pancreatic juice and pancreatic tissue of patients with chronic pancreatitis.
abstract: BACKGROUND
  The predominance of secretory IgA (S-IgA) in intestinal secretions compared with blood is well established, but concentrations of this protein in pancreatic juice and its origin, especially in chronic pancreatitis, are unknown.
  
  
  OBJECTIVE
  To investigate the role of S-IgA in chronic pancreatitis.
  
  
  METHODS
  Twenty one patients with chronic pancreatitis (group I), three patients with proven malignancies (group II), and 12 patients without pancreatic disease (group III).
  
  
  METHODS
  Pure human pancreatic juice was collected endoscopically in four fractions after consecutive stimulation with secretin and cholecystokinin (CCK). Samples were analysed for S-IgA, protein, trypsinogen, and proteolytic activity.
  
  
  RESULTS
  The S-IgA level was significant increased in fraction 1 of pancreatic juice of group I (1210 (1411) ng/ml) compared with controls (33 (70) ng/ml). Protein concentrations and trypsinogen content were lower in group I than in the other groups. Proteolytic activity could be observed in 53% of all 133 pancreatic juice samples, but in 87% of fraction 1. In pancreatic tissue of three patients with chronic pancreatitis both IgA and secretory component were detected by immunohistology. Expression of the secretory component by human pancreatic epithelial cells was increased in patients with chronic pancreatitis compared with normal controls. The concentration of S-IgA in pancreatic juice did not correlate with the serum S-IgA level. In contrast, serum levels of S-IgA were decreased in patients with chronic pancreatitis.
  
  
  CONCLUSIONS
  There are high levels of S-IgA in human pancreatic juice following chronic inflammation and a protective role is suggested for this immunoglobulin.
authors.affiliation: Department of Internal Medicine, University of Rostock, Germany.
authors.forename: J
authors.initials: J
authors.lastname: Emmrich
authors.forename: M
authors.initials: M
authors.lastname: Seyfarth
authors.forename: P
authors.initials: P
authors.lastname: Conradi
authors.forename: F
authors.initials: F
authors.lastname: Plath
authors.forename: G
authors.initials: G
authors.lastname: Sparmann
authors.forename: M
authors.initials: M
authors.lastname: Löhr
authors.forename: S
authors.initials: S
authors.lastname: Liebe
mesh_terms: D000328:Adult; D000368:Aged; D015415:Biomarkers; D002908:Chronic Disease; D004847:Epithelial Cells / Q000276:immunology; D005260:Female; D006801:Humans; D007070:Immunoglobulin A / Q000032:analysis; D007071:Immunoglobulin A, Secretory / Q000032:analysis*; D007150:Immunohistochemistry; D008297:Male; D008875:Middle Aged; D010179:Pancreas / Q000276:immunology*; D010189:Pancreatic Juice / Q000276:immunology*; D010195:Pancreatitis / Q000276:immunology*; D018709:Statistics, Nonparametric
chemical_list: D015415:Biomarkers; D007070:Immunoglobulin A; D007071:Immunoglobulin A, Secretory