Modification of postradiative changes of nucleic acids by the bacterial extract broncho-vaxom in rat tissues.
We have studied the influence of immunomodulator Broncho-Vaxom on the concentration and the total content of RNA and DNA in the bone marrow, spleen and regenerating liver (after partial hepatectomy) in rats irradiated with doses of 3 Gy, 6 Gy or 9 Gy of gamma radiation. Animals in all groups were partially hepatectomized (2/3 of liver mass removed) within 30 min or on the 6th and 13th day after irradiation and they were examined 30 h after resection, i.e., on the 1st, 7th and 14th day after irradiation. Broncho-Vaxom was administered intraperitoneally at the dose of 1.5 mg/rat 24 h before irradiation. We have found that administration of Broncho-Vaxom decreased the loss of RNA and DNA induced by radiation in the bone marrow and partly also in the spleen of rats irradiated with doses 3 and 6 Gy over the whole examined period with a maximum on the 14th day after irradiation. The effect of Broncho-Vaxom was less noticeable in the regenerating liver and appeared especially on the 7th day after irradiation.
Haková
H
H
Department of Cellular and Molecular Biology, Faculty of Sciences, P. J. Safárik University, Kosice, Slovakia.
Misúrová
E
E
Kropácová
K
K
D000276:Adjuvants, Immunologic / Q000494:pharmacology*; D000818:Animals; D001419:Bacteria; D001853:Bone Marrow / Q000528:radiation effects; D002457:Cell Extracts; D004247:DNA / Q000187:drug effects / Q000528:radiation effects*; D005720:Gamma Rays; D008115:Liver Regeneration / Q000528:radiation effects; D012313:RNA / Q000187:drug effects / Q000528:radiation effects*; D011837:Radiation-Protective Agents / Q000494:pharmacology*; D051381:Rats; D017208:Rats, Wistar; D013154:Spleen / Q000528:radiation effects; D000095983:Bacterial Lysates
D000276:Adjuvants, Immunologic; D002457:Cell Extracts; D004247:DNA; D012313:RNA; D011837:Radiation-Protective Agents; C030259:Broncho-Vaxom; D000095983:Bacterial Lysates