Mechanistic analogy between metal ion-catalyzed self-splicing of protein and RNA.
Recent studies have shown that RNA hydrolysis is remarkably promoted by metal ions and suitably designed metal complexes. We have found that autocleavage of dipeptides X-Ser and a tripeptide Gly-Ser-Ala is catalyzed by Zn(II) at 70 degrees C and pH 7.0. A mechanistic analogy between metal ion-catalyzed hydrolysis of peptides and RNA is suggested.
Takarada
T
T
Department of Chemistry and Biotechnology, Graduate School of Engineering, University of Tokyo, Japan.
Yamamura
A
A
Yashiro
M
M
Komiyama
M
M
D002104:Cadmium; D002384:Catalysis; D002851:Chromatography, High Pressure Liquid; D004151:Dipeptides / Q000737:chemistry*; D006868:Hydrolysis; D007700:Kinetics; D007811:Lanthanum; D008670:Metals; D009842:Oligopeptides / Q000737:chemistry*; D012313:RNA / Q000737:chemistry*; D013329:Structure-Activity Relationship; D015032:Zinc
D004151:Dipeptides; D008670:Metals; D009842:Oligopeptides; D002104:Cadmium; D012313:RNA; D007811:Lanthanum; D015032:Zinc