Isolation and sequence analysis of metalloprotease gene from Vibrio mimicus.
The vmc gene encoding a metalloprotease of Vibrio mimicus (ATCC 33653) was cloned in Escherichia coli and sequenced. The vmc gene contained 1884 nt sequence which codes a polypeptide of 628 amino acids with a predicted molecular mass of 71,275 Da. The deduced amino acid sequence had the similarity of 68.5% with V. parahaemolyticus metalloprotease. The consensus sequence of a zinc binding motif (HEXXH) was identified to be HEYTH. The zymography analysis showed a gelatinolytic protein band around molecular mass of 61 kDa, and this result suggested that the cloned metalloprotease may undergo processing during secretion.
Lee
J H
JH
Department of Biotechnology and Bioengineering, Pukyong National University, Pusan, South Korea.
Kim
G T
GT
Lee
J Y
JY
Jun
H K
HK
Yu
J H
JH
Kong
I S
IS
D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000235:genetics*; D001483:Base Sequence; D003001:Cloning, Molecular; D005798:Genes, Bacterial; D008666:Metalloendopeptidases / Q000235:genetics*; D008969:Molecular Sequence Data; D016415:Sequence Alignment; D017421:Sequence Analysis; D014733:Vibrio / Q000235:genetics*
D001426:Bacterial Proteins; D008666:Metalloendopeptidases