Prompt activation of telomerase by chemical carcinogens in rats detected with a modified TRAP assay.
The maintenance of telomere length is crucial for survival of cells. Telomerase is an RNA-containing reverse transcriptase, which is responsible for elongation of shortened telomeres. Telomerase reactivation has been suggested to be involved in malignant progressions. To study on the involvement of telomerase activation in in vivo carcinogenesis, we first modified the original TRAP assay by changing the primer designs and the labeling method of PCR products to an end-labeling method. Second, we investigated the activation of telomerase in different organs after treatments of rats with various chemical carcinogens. Very early after the beginning of the treatment, telomerase activity in the liver, kidney, and lung was increased. In most cases, telomerase activation occurred in the primary or favorite target organs. The present results suggest that telomerase activation occurs promptly when animals are exposed to chemical carcinogens, which may contribute to in vivo chemical carcinogenesis.
Miura
M
M
Department of Oral Surgery, School of Health Sciences, University of Occupational and Environmental Health, Kitakyushu City, Japan. SGW01073@niftyserve.or.jp
Karasaki
Y
Y
Abe
T
T
Higashi
K
K
Ikemura
K
K
Gotoh
S
S
D000818:Animals; D001483:Base Sequence; D002273:Carcinogens / Q000633:toxicity*; D002460:Cell Line; D004247:DNA / Q000235:genetics; D017931:DNA Primers / Q000235:genetics; D004789:Enzyme Activation / Q000187:drug effects; D006801:Humans; D007668:Kidney / Q000187:drug effects / Q000201:enzymology; D008099:Liver / Q000187:drug effects / Q000201:enzymology; D008114:Liver Neoplasms, Experimental / Q000139:chemically induced / Q000201:enzymology / Q000235:genetics; D008168:Lung / Q000187:drug effects / Q000201:enzymology; D016133:Polymerase Chain Reaction / Q000379:methods*; D051381:Rats; D017208:Rats, Wistar; D012091:Repetitive Sequences, Nucleic Acid; D019098:Telomerase / Q000378:metabolism*
D002273:Carcinogens; D017931:DNA Primers; D004247:DNA; D019098:Telomerase