title: Healing of tibial fractures is not impaired after acute hindlimb ischemia in rats.
abstract: The influence of transient circulatory arrest on the healing of closed tibial fractures was investigated in rats by the use of a hindlimb tourniquet technique. Twenty-four animals were randomly divided into three groups. In all animals, the left lower leg was fractured and fixed with an intramedullary nail system. In the ischemic group, complete acute transient ischemia for 4.5 h and neurapraxia of the sciatic and femoral nerves were induced prior to fracture. In the neurapraxia group, the sciatic and femoral nerves were crushed with forceps before fracture. In the control group, no other intervention than fracture was made. The rats of the control group ambulated normally 3-4 days after the operation. The animals of the ischemic and neurapraxia groups resumed normal weight-bearing after about 3 weeks. After 6 weeks, all animals were killed, and mechanical strength and bone mineral turnover of the healing tibia as well as blood flow of the bone and musculature were evaluated. The weight of the tibia and the corresponding anterior tibial muscle in the ischemic and neurapraxia animals were reduced compared with the control rats. Bone mineral turnover was found to be lower in the ischemic group. There were no differences between the groups in mechanical strength nor in blood circulation of bone and muscle. In conclusion, complete, acute hindlimb ischemia for 4.5 h in rats did not cause delayed healing of closed tibial fractures.
authors.affiliation: Institute for Surgical Research, National Hospital, University of Oslo, Norway.
authors.forename: T
authors.initials: T
authors.lastname: Kase
authors.forename: S
authors.initials: S
authors.lastname: Skjeldal
authors.forename: L
authors.initials: L
authors.lastname: Nordsletten
authors.forename: O
authors.initials: O
authors.lastname: Reikerås
mesh_terms: D000818:Animals; D015519:Bone Density / Q000502:physiology; D005267:Femoral Nerve / Q000503:physiopathology; D005594:Fracture Fixation, Intramedullary; D017102:Fracture Healing / Q000502:physiology*; D005596:Fractures, Closed / Q000503:physiopathology; D006614:Hindlimb / Q000098:blood supply*; D007511:Ischemia / Q000503:physiopathology*; D008297:Male; D009121:Muscle Denervation; D051381:Rats; D017208:Rats, Wistar; D012584:Sciatic Nerve / Q000503:physiopathology; D013978:Tibial Fractures / Q000503:physiopathology*; D014111:Tourniquets; D016474:Weight-Bearing / Q000502:physiology