title: Novel detection system of flow injection analysis (1). The existence of significant relation between secondary structure of DNA and sensitivity in signal detection.
abstract: Polymerase Chain Reaction (PCR) products was detected quantitatively using a flow injection type sensor, based on Surface Plasmon Resonance (SPR). We used asymmetric PCR to amplify the two kinds of products; their DNA lengths are different. This novel design permitted us not only to detect PCR products with high-sensitivity, but also to develop a rapid DNA detection system for the sense of the genetic pathogen.
authors.affiliation: National Space Development Agency of Japan, Ibaraki, Japan.
authors.forename: S
authors.initials: S
authors.lastname: Sawata
authors.forename: E
authors.initials: E
authors.lastname: Kai
authors.forename: K
authors.initials: K
authors.lastname: Ikebukuro
authors.forename: T
authors.initials: T
authors.lastname: Iida
authors.forename: T
authors.initials: T
authors.lastname: Honda
authors.forename: I
authors.initials: I
authors.lastname: Karube
mesh_terms: D001427:Bacterial Toxins / Q000235:genetics; D004247:DNA / Q000737:chemistry*; D004269:DNA, Bacterial / Q000032:analysis / Q000737:chemistry*; D004926:Escherichia coli / Q000235:genetics; D005798:Genes, Bacterial; D009690:Nucleic Acid Conformation; D016133:Polymerase Chain Reaction / Q000379:methods; D012680:Sensitivity and Specificity; D022641:Shiga Toxin 2; D013057:Spectrum Analysis / Q000379:methods
chemical_list: D001427:Bacterial Toxins; D004269:DNA, Bacterial; D022641:Shiga Toxin 2; D004247:DNA