title: [Prevalence of subgroups, serotypes and electropherotypes of human rotavirus in Cumaná, Venezuela].
abstract: From June 1992 to May 1993, rotaviruses were detected by an immunoenzymatic assay, in 159 (49.5%) of 321 children admitted to the hospital with acute diarrhea. Of the 159 cases ELISA positive, 80 samples were chosen at random to investigate subgroups and serotypes of group A human rotavirus. By the ELISA, test 9 (11.3%) of the strains were subgroup I, 46 (57.5%) were subgroup II, and 25 (31.3%) could not be grouped. The serotype G1 was identified in 52 cases (65%), G2 in 11 cases (13.8%), G3 in 1 case (1.2%), and 7 cases (8.8%) showed more than one serotype. By electrophoretic analysis of viral RNA, 137 (42.7%) of the samples exhibited an RNA pattern. The long pattern (59.1%) prevailed over the short pattern (35.8%), and by coelectrophoresis 8 different electropherotypes were found throughout the period of study. These results illustrate the great variety of rotavirus strains in this region of the country.
authors.affiliation: Postgrado en Biología Aplicada, Universidad de Oriente, Núcleo de Sucre, Cumaná, Estado Sucre, Venezuela.
authors.forename: A J
authors.initials: AJ
authors.lastname: Maldonado
authors.forename: J W
authors.initials: JW
authors.lastname: Bastardo
mesh_terms: D002675:Child, Preschool; D004279:DNA, Viral / Q000032:analysis*; D003968:Diarrhea, Infantile / Q000821:virology; D004591:Electrophoresis, Polyacrylamide Gel; D004797:Enzyme-Linked Immunosorbent Assay; D005243:Feces / Q000821:virology; D006801:Humans; D007223:Infant; D007231:Infant, Newborn; D012401:Rotavirus / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification; D012400:Rotavirus Infections / Q000821:virology; D012621:Seasons; D012703:Serotyping
chemical_list: D004279:DNA, Viral
vernacular_title: Prevalencia de subgrupos, serotipos y electroferotipos de rotavirus humanos en Cumaná, Venezuela.