title: Comparison between functional characteristics of healthy and pathological human retinal Müller glial cells.
abstract: Enzymatically isolated, noncultured Müller glial cells from human organ donor and patient eyes were studied using the whole-cell-voltage-clamp and the patch-clamp technique. The patients suffered mainly from choroidal melanomas, retinal detachment due to proliferative vitreorentinopathy, glaucomas, and perforating eye injuries. The organ donor eyes were used as a source of corneas for corneal transplantation. Use of the human retinal tissue was approved by the Ethics Committee of the School of Medicine, University of Leipzig. Most of the patient Müller cells showed a marked or complete loss of inwardly rectifying K+ currents, causing a dramatic increase in the input resistance. The zero current potential of the patient Müller cells, which is equivalent to the membrane potential, was significantly reduced (depolarized) as compared with the donor Müller cells. In contrast to the K+ current loss, the Na+ current density was significantly higher in patient Müller cells than in donor Müller cells; the number of Müller cells depicting Na+ currents increased from 33% (3 pA/pF) in donor Müller cells to 85% (about 12pA/pF) in patient Müller cells. Application of glutamate to the Müller cells generated a glutamate-transporter-mediated current, such as that seen in other species. A highly significant increase was noted for the high-affinity Na+-dependent glutamate-transporter-current density in patient Müller cells compared with donor cells. The application of gamma-aminobutyric acid (GABA) evoked, in addition to the GABA transporter currents already known from Müller cells of other mammalian species, GABA(A)-receptor mediated currents in human Müller cells. We found that GABA(A) receptors are expressed in human Müller cells, but not in other nonprimate mammals. Whether a difference exists between the GABA(A) current density in donor and patient Müller cells remains to be seen. The results concerning the disappearance of K+ currents and diminution of the membrane potential may demonstrate early glial changes that may possibly precede pathological neuronal changes, at least in retinas from eyes with choroidal melanomas. In later stages of the diseases, the glial changes may be deleterious for the neurons, because they could diminish glutamate uptake due to the depolarized membrane potential. However, increased extracellular glutamate concentration is toxic for most neurons.
authors.affiliation: Paul Flechsig Institute for Brain Research, Department of Neurophysiology, University of Leipzig, Germany.
authors.forename: W
authors.initials: W
authors.lastname: Reichelt
authors.forename: T
authors.initials: T
authors.lastname: Pannicke
authors.forename: B
authors.initials: B
authors.lastname: Biedermann
authors.forename: M
authors.initials: M
authors.lastname: Francke
authors.forename: F
authors.initials: F
authors.lastname: Faude
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000818:Animals; D001692:Biological Transport; D005260:Female; D018698:Glutamic Acid / Q000494:pharmacology; D006168:Guinea Pigs; D006736:Horses; D006801:Humans; D008297:Male; D008564:Membrane Potentials; D051379:Mice; D008875:Middle Aged; D009457:Neuroglia / Q000187:drug effects / Q000502:physiology*; D018408:Patch-Clamp Techniques; D011188:Potassium / Q000378:metabolism; D011817:Rabbits; D051381:Rats; D011963:Receptors, GABA-A / Q000378:metabolism; D012160:Retina / Q000187:drug effects / Q000502:physiology*; D012164:Retinal Diseases / Q000378:metabolism*; D012964:Sodium / Q000378:metabolism; D013552:Swine; D005680:gamma-Aminobutyric Acid / Q000378:metabolism
chemical_list: D011963:Receptors, GABA-A; D018698:Glutamic Acid; D005680:gamma-Aminobutyric Acid; D012964:Sodium; D011188:Potassium