Effect of peripheral 2-DG on opioid and neuropeptide Y gene expression.
It is well known that 2-Deoxy-d-glucose (2-DG) blocks intracellular utilization of glucose and increases food intake. The aim of the present study was to determine whether administration of 2-DG alters gene expression of the orexigenic peptides, neuropeptide Y (NPY) and endogenous opioids, in the arcuate nucleus of the hypothalamus (ARC). Male Sprague-Dawley rats were injected peripherally (i.p.) with 2-DG (200 or 400 mg/kg body weight) and were sacrificed at 2 or 6 h post injection. Half of the animals were given ad libitum access to food whereas the other half of the animals were food-deprived. 2-DG increased food intake fourfold compared to saline injected animals, but did not affect NPY mRNA levels after 2 h. Messenger RNA levels of ProDynorphin (proDYN), but not pro-opiomelanocortin (POMC) nor proEnkephalin (proENK) were significantly decreased 2 h after 2-DG injection. Administration of 400 mg/kg of 2-DG increased mRNA levels of NPY in the arcuate nucleus after six h, but only in those animals not receiving food.
Giraudo
S Q
SQ
Minnesota Obesity Center, V.A. Medical Center, Minneapolis, MN 55417, USA.
Kim
E M
EM
Grace
M K
MK
Billington
C J
CJ
Levine
A S
AS
D000818:Animals; D000963:Antimetabolites / Q000008:administration & dosage / Q000494:pharmacology*; D003847:Deoxyglucose / Q000008:administration & dosage / Q000494:pharmacology*; D004435:Eating / Q000187:drug effects; D004745:Enkephalins / Q000096:biosynthesis; D015870:Gene Expression / Q000187:drug effects*; D008297:Male; D009478:Neuropeptide Y / Q000096:biosynthesis*; D018847:Opioid Peptides / Q000096:biosynthesis*; D010286:Paraventricular Hypothalamic Nucleus / Q000187:drug effects / Q000378:metabolism; D011333:Pro-Opiomelanocortin / Q000096:biosynthesis; D011498:Protein Precursors / Q000096:biosynthesis; D012333:RNA, Messenger / Q000096:biosynthesis; D051381:Rats; D017207:Rats, Sprague-Dawley
D000963:Antimetabolites; D004745:Enkephalins; D009478:Neuropeptide Y; D018847:Opioid Peptides; D011498:Protein Precursors; D012333:RNA, Messenger; C029992:proenkephalin; D011333:Pro-Opiomelanocortin; C033660:preproenkephalin; D003847:Deoxyglucose