title: Source of reactive oxygen species in anti-Thy1 nephritis.
abstract: In proliferative glomerulonephritis, both macrophages and mesangial cells generate reactive oxygen species (ROS), contributing to the development of glomerular injury. We have attempted to determine which cell produces ROS during anti-Thy1 nephritis (ATN) in rats. The generation of ROS was studied using luminol amplified chemiluminescence (GCL) on isolated glomeruli. Immunohistochemical studies used avidin-biotin complex (ABC) to label macrophages and mesangial cells. Immediately after ATN induction, mesangiolysis and infiltration with ED-1 positive cells (referred to as macrophage) was noted with a peak at day 1. After day 4, mesangial proliferation appeared with a decrease of the ED-1 positive cells and a prominent increase of PCNA positive cells (regarded as mesangial cells). In the early phase of ATN, GCL, reflecting ROS generation, increased along with the appearance of ED-1 positive cells. GCL subsequently decreased as mesangial cells increased. This suggested that macrophage were the principal participants in ROS generation in the early phase of ATN although mesangial cells cannot be completely disregarded in the generation of ROS and development of glomerular injury.
authors.affiliation: Department of Medicine, Teikyo University School of Medicine, Tokyo, Japan.
authors.forename: K
authors.initials: K
authors.lastname: Nakamura
authors.forename: M
authors.initials: M
authors.lastname: Oka
authors.forename: M
authors.initials: M
authors.lastname: Shirai
authors.forename: Y
authors.initials: Y
authors.lastname: Igarashi
authors.forename: K
authors.initials: K
authors.lastname: Kojima
authors.forename: O
authors.initials: O
authors.lastname: Kaneko
authors.forename: N
authors.initials: N
authors.lastname: Hamada
authors.forename: J
authors.initials: J
authors.lastname: Mera
authors.forename: H
authors.initials: H
authors.lastname: Masaoka
authors.forename: M
authors.initials: M
authors.lastname: Nagase
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000008:administration & dosage*; D002455:Cell Division; D005500:Follow-Up Studies; D005920:Glomerular Mesangium / Q000378:metabolism / Q000473:pathology; D005921:Glomerulonephritis / Q000276:immunology / Q000378:metabolism* / Q000473:pathology; D007124:Immunoenzyme Techniques; D007518:Isoantibodies / Q000008:administration & dosage*; D008163:Luminescent Measurements; D008264:Macrophages / Q000378:metabolism* / Q000473:pathology; D008297:Male; D051381:Rats; D017208:Rats, Wistar; D017382:Reactive Oxygen Species / Q000378:metabolism*; D018800:Thy-1 Antigens / Q000276:immunology
chemical_list: D000911:Antibodies, Monoclonal; D007518:Isoantibodies; D017382:Reactive Oxygen Species; D018800:Thy-1 Antigens; C035678:anti-Thy antibody