title: [Resistance of Haemophilus sp. to antibiotics].
abstract: The present study was undertaken to determine the in vitro drug resistance of Haemophilus influenzae (68 isolates) and H. parainfluenzae (17 isolates). The tests susceptibility to Ampicillin, Amoxicilin/Clavulanic Acid, Cefaclor, Cefuroxime, Cotrimoxazole, Aztreonam, Ceftriaxone, Tetracycline, Ciprofloxacin, Rifampicin and Chloramphenicol were performed with a standard disk-diffusion method. The NCCLS methodology and susceptibility interpretative criteria were applied as described by the disk manufacturer. Beta-lactamase production was detected with nitrocefin impregnated disk (Cefinase, BBL Microbiology System). Resistance in nosocomially acquired Haemophilus isolates to several antibiotics was observed. Of the Haemophilus isolates 28.2% were Ampicillin in resistant, all were susceptible to the combination of Amoxicillin/Clavulanic acid. The Ampicillin-resistant strains were beta-lactamase producers. We observed the high resistance (70.1%) to Tetracycline and (28.2%) to SXT (Cotrimoxazole). All isolates of Haemophilus were susceptible to Ciprofloxacin. The low resistance percentages to Rifampin (1.2%), Aztreonam (3.5%) and Chloramphenicol (3.5%) was observed.
authors.affiliation: Katedry i Zakładu Mikrobiologii Państwowego Szpitala Klinicznego Akademii Medycznej w Bydgoszczy.
authors.forename: G
authors.initials: G
authors.lastname: Janicka
authors.forename: E
authors.initials: E
authors.lastname: Gospodarek
authors.forename: A
authors.initials: A
authors.lastname: Mikucka
authors.forename: M
authors.initials: M
authors.lastname: Białek
authors.forename: I
authors.initials: I
authors.lastname: Wojak
authors.forename: D
authors.initials: D
authors.lastname: Krawiecka
mesh_terms: D004352:Drug Resistance, Microbial; D006190:Haemophilus / Q000145:classification / Q000187:drug effects* / Q000201:enzymology; D006801:Humans; D008826:Microbial Sensitivity Tests; D011044:Poland; D013045:Species Specificity; D001618:beta-Lactamases / Q000378:metabolism
chemical_list: D001618:beta-Lactamases
vernacular_title: Oporność Haemophilus sp. na antybiotyki.