title: Analysis of a vaccinia virus mutant expressing a nonpalmitylated form of p37, a mediator of virion envelopment.
abstract: Vaccinia virus encodes a 37-kDa palmitylated protein (p37) that is required for envelopment, translocation, and cell-to-cell spread of virions. We have analyzed the biological significance of the palmitate modification by constructing a recombinant vaccinia virus that expresses a nonpalmitylated p37 and comparing its biological activity to that of the wild-type virus. The mutant virus is inefficient at cell-to-cell spread and does not produce or release enveloped virions, although it produces normal amounts of nonenveloped virions. Furthermore, the mutant virus is not able to nucleate actin to propel itself through and out of the cell, a function requiring the indirect participation of p37. The deficiency in protein function appears to result from a lack of appropriate targeting to the membranes of the trans-Golgi network (TGN) which leaves p37 soluble in the cytoplasm. We conclude that the palmitate moiety is necessary for targeting or anchoring p37 to the TGN membrane, where, along with other vaccinia virus-encoded proteins, p37 is involved in the complex process of virion envelopment and release.
authors.affiliation: Center for Gene Research and Biotechnology, Department of Microbiology, Oregon State University, Corvallis, Oregon 97331-3804, USA.
authors.forename: D W
authors.initials: DW
authors.lastname: Grosenbach
authors.forename: D E
authors.initials: DE
authors.lastname: Hruby
mesh_terms: D000818:Animals; D002460:Cell Line; D004274:DNA, Recombinant / Q000235:genetics; D004279:DNA, Viral / Q000235:genetics*; D008565:Membrane Proteins / Q000502:physiology*; D009154:Mutation; D011995:Recombination, Genetic; D014616:Vaccinia virus / Q000502:physiology*; D014759:Viral Envelope Proteins / Q000502:physiology*; D014771:Virion / Q000502:physiology; D014779:Virus Replication / Q000235:genetics*
chemical_list: D004274:DNA, Recombinant; D004279:DNA, Viral; D008565:Membrane Proteins; D014759:Viral Envelope Proteins; C084759:p37 protein, Vaccinia virus