Immunoradiometric assay for the N-terminal fragment of proatrial natriuretic peptide in human plasma.
Recently, the N-terminal fragment of proatrial natriuretic peptide (N-terminal proANP) has been proposed as a marker of chronic congestive heart failure. In this study, we established a two-step immunoradiometric assay using monoclonal antibodies and synthetic N-terminal proANP (1-67) as a standard. It allows us to measure plasma N-terminal proANP in only 4 h without prior extraction. The detection limit of this assay was 15 pmol/L for a 100 microL sample of plasma. Within-run CVs ranged from 1.7% to 2.9% and between-run CVs ranged from 4.2% to 5.1%. The dilution curves of plasma samples showed good linearity and analytical recovery was 89-104%. The mean (+/-SD) N-terminal proANP in plasma of 33 healthy subjects was 188 (+/-71) pmol/L and 1030 (+/-411) pmol/L in 25 patients with heart failure. Our immunoradiometric assay is rapid and precise enough for routine determination of N-terminal proANP in human plasma.
Numata
Y
Y
Diagnostic Science Division, Shionogi & Company, Osaka, Japan. yoshito.numata@shionogi.co.jp
Dohi
K
K
Furukawa
A
A
Kikuoka
S
S
Asada
H
H
Fukunaga
T
T
Taniguchi
Y
Y
Sasakura
K
K
Tsuji
T
T
Inouye
K
K
Yoshimura
M
M
Itoh
H
H
Mukoyama
M
M
Yasue
H
H
Nakao
K
K
D000911:Antibodies, Monoclonal / Q000276:immunology; D009320:Atrial Natriuretic Factor / Q000097:blood* / Q000276:immunology; D015415:Biomarkers / Q000097:blood; D002850:Chromatography, Gel; D003429:Cross Reactions; D004355:Drug Stability; D004492:Edetic Acid; D000939:Epitopes / Q000276:immunology; D006333:Heart Failure / Q000097:blood; D006461:Hemolysis; D006801:Humans; D015592:Immunoradiometric Assay / Q000379:methods; D011498:Protein Precursors / Q000097:blood* / Q000276:immunology; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
D000911:Antibodies, Monoclonal; D015415:Biomarkers; D000939:Epitopes; C099254:N-terminal proatrial natriuretic peptide; D011498:Protein Precursors; D009320:Atrial Natriuretic Factor; D004492:Edetic Acid