[The effect of interleukin (IL) 12 on hemopoiesis in irradiated mice].
OBJECTIVE
To investigate the regulating effect of IL-12 on hemopoiesis.


METHODS
cDNA coding for the two subunits of murine IL-12 were cloned into two different eukaryotic expression vectors and transduced into a same NIH3T3 cell line, resulting in two engineered fibroblast clones secreting IL-12. Cells were mixed with collagens and inoculated into the peritoneal cavities of whole-body-irradiated mice.


RESULTS
On day 10 after treatment, endogenous splenic colony-forming-unit (CFU-S) counts in mice treated with IL-12-secreting NIH3T3 cells were higher than those of mice accepting parental cells or untreated irradiated mice. This difference was statistically significant in both 6.0 Gy irradiated mice and 7.0 Gy group mice. Histopathological studies showed that NIH3T3-secreted IL-12 alleviates the injuries caused by irradiation in both bone marrow and spleen, activates hemopoietic cells, increases the number and scale of hemopoietic clusters, hence enhancing hemopoietic function including erythropoiesis, granulopoiesis, and megakaryocytopoiesis.


CONCLUSIONS
IL-12 up-regulates and promotes the recovery of hemopoietic function in irradiated mice.
Wang
Y
Y
Beijing Institute of Basic Medical Sciences.
Tang
P
P
Guo
N
N
D016475:3T3 Cells / Q000378:metabolism; D000818:Animals; D003001:Cloning, Molecular; D003037:Cobalt Radioisotopes; D005260:Female; D006412:Hematopoietic Stem Cells / Q000187:drug effects* / Q000528:radiation effects*; D018664:Interleukin-12 / Q000096:biosynthesis / Q000235:genetics / Q000494:pharmacology*; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D014162:Transfection; D014916:Whole-Body Irradiation
D003037:Cobalt Radioisotopes; D018664:Interleukin-12