Differential genome analysis of bacteria by genomic subtractive hybridization and pulsed field gel electrophoresis.
A comprehensive analysis of the differences between the genomes of two closely related bacterial strains should give insight into the molecular basis of their individual phenotypic and genotypic characteristics. Here we present an integrative approach including two different strategies for the thorough investigation of genomic divergence. We have combined two techniques including genomic subtractive hybridization and comparative genome mapping by pulsed field gel electrophoresis (PFGE) techniques. The subtractive method for which a protocol is given herein results in the production of a library of specific DNA sequence tags present only in one strain, while the construction of macrorestriction maps of the bacterial chromosomes yields data about the overall genome organization and the arrangement and distance of gene loci. Comparison of the physical and genetic maps and determination of the map positions of the strain-specific DNA sequences reveals gross chromosomal modifications, insertions or deletions of additional genetic material, and transpositional events. The further investigation of the strain-specific regions yields information about the nature and origin of the acquired DNA and their influence on the evolution of the individual bacterial genome. The two methods were applied to differential genome analysis of clonal divergence in Pseudomonas aeruginosa choosing two clone C isolates from diverse habitats.
Schmidt
K D
KD
Klinische Forschergruppe, Zentrum Biochemie, Medizinische Hochschule Hannover, Germany.
Schmidt-Rose
T
T
Römling
U
U
Tümmler
B
B
D002876:Chromosomes, Bacterial; D004269:DNA, Bacterial / Q000032:analysis; D016521:Electrophoresis, Gel, Pulsed-Field / Q000379:methods*; D016680:Genome, Bacterial; D009693:Nucleic Acid Hybridization / Q000379:methods*; D011550:Pseudomonas aeruginosa / Q000235:genetics*; D015183:Restriction Mapping
D004269:DNA, Bacterial