[Effect of 764-3 and ligustrazine on collagen content of extrapulmonary arteries during chronic hypoxia].
OBJECTIVE
Effect of two antifibrotic Chinese drugs, 764-3 and ligustrazine, on collagen content in chronic hypoxic pulmonary hypertensive extrapulmonary arterial wall were examined.


METHODS
Since collagen contains more than 10% hydroxyproline by weight and in other proteins there are almost no hydroxyproline, collagen content was expressed as micrograms of hydroxyproline. Hydroxyproline was determined by calorimetric method after oxidized by chloramine T.


RESULTS
Both 764-3 and ligustrazine significantly inhibited the increase of pulmonary arterial pressure and elevation of hydroxyproline content in extrapulmonary arterial wall during chronic hypoxia.


CONCLUSIONS
764-3 and ligustrazine may be two hopeful therapeutic drugs for chronic hypoxic pulmonary hypertension.
Yan
Y
Y
Institute of Basic Medical Science, Chinese Academy of Medical Sciences, Beijing.
Chen
X
X
Sun
R
R
D000818:Animals; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000494:pharmacology*; D003094:Collagen / Q000378:metabolism*; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D006909:Hydroxyproline / Q000378:metabolism; D006976:Hypertension, Pulmonary / Q000378:metabolism*; D000860:Hypoxia / Q000378:metabolism*; D008297:Male; D011651:Pulmonary Artery / Q000378:metabolism*; D011719:Pyrazines / Q000494:pharmacology*; D051381:Rats; D017208:Rats, Wistar
D000894:Anti-Inflammatory Agents, Non-Steroidal; D004365:Drugs, Chinese Herbal; C079053:IH 764-3; D011719:Pyrazines; D003094:Collagen; D006909:Hydroxyproline; C017953:tetramethylpyrazine