TBJ murine neuroblastoma resists dibutyryl cyclic AMP-induced differentiation and effects on metalloproteinase secretion.
BACKGROUND
Nonmetastatic C1300 murine neuroblastoma and TBJ, a metastatic variant, exhibit different mechanisms of metastasis and invasion.


METHODS
In this study, they were examined for response to 1 mmol/L dibutyryl cyclic AMP in vitro.


RESULTS
(1) dCAMP induced morphological differentiation in 20% to 40% of C1300 cells, whereas TBJ resisted differentiation. (2) Untreated TBJ expressed 92-kDa metalloproteinase, whereas C1300 expressed none. These results were not affected by dCAMP. (3) Growth curves of untreated C1300 and TBJ were exponential. Dibutyryl CAMP induced a plateau in C1300 growth that was confirmed by increased S-phase cells by cell cycle analysis.


CONCLUSIONS
TBJ growth was unaffected by dCAMP. Apoptosis, as assayed by Hoechst-merocyanine staining test, was not involved in the growth plateau.
Fowler
C
C
Department of Surgery, LP Markey Cancer Center, University of Kentucky Medical Center, Lexington 40536, USA.
Zimmer
C
C
Zimmer
S
S
D000818:Animals; D017209:Apoptosis; D003994:Bucladesine / Q000494:pharmacology*; D002454:Cell Differentiation / Q000187:drug effects; D002455:Cell Division / Q000187:drug effects; D066298:In Vitro Techniques; D008666:Metalloendopeptidases / Q000096:biosynthesis*; D051379:Mice; D009447:Neuroblastoma / Q000473:pathology*; D014407:Tumor Cells, Cultured
D003994:Bucladesine; D008666:Metalloendopeptidases