Sequence-selective RNA scission by oligoamine--DNA conjugates (1).
Ethylenediamine and diethylenetriamine, which are active for RNA hydrolysis, are attached to the 5'-ends of synthetic oligonucleotides by urethane linkages. The resultant artificial ribonucleases selectively hydrolyze the substrate RNA at the target phosphodiester linkage. Acid/base cooperation in the oligoamines for the catalysis is confirmed in terms of kinetic evidences.
Kuzuya
A
A
Department of Chemistry and Biotechnology, Graduate School of Engineering, University of Tokyo, Japan.
Azuma
Y
Y
Inokawa
T
T
Yoshinari
K
K
Komiyama
M
M
D001483:Base Sequence; D004247:DNA; D005029:Ethylenediamines; D006868:Hydrolysis; D007202:Indicators and Reagents; D009693:Nucleic Acid Hybridization; D009838:Oligodeoxyribonucleotides; D011073:Polyamines; D012313:RNA / Q000737:chemistry*
D005029:Ethylenediamines; D007202:Indicators and Reagents; D009838:Oligodeoxyribonucleotides; D011073:Polyamines; C005391:diethylenetriamine; C031234:ethylenediamine; D012313:RNA; D004247:DNA