Detection of metals in tissues, cells and subcellular organelles.
The current state of art in the detection of metals in tissues, cells and their subcellular fractions has been reviewed. Both classic chemical techniques (including titration, spectrophotometric, electrochemical and isotope methods, neutron activation analysis, mass spectrometry, inductively coupled plasma) and cell biology techniques (i.e. conventional histochemical methods, autometallography, autoradiography and microanalytical techniques using beam of electrons or protons, laser, X-ray or ion beam) were discussed. This survey of analytical techniques is, wherever possible, illustrated by examples documenting how our knowledge on metals in pigment cells and their subcellular fractions has been growing.
Borovanský
J
J
2nd Department of Medical Chemistry and Biochemistry, 1st Faculty of Medicine, Charles University, Prague, Czech Republic.
D000818:Animals; D006801:Humans; D008670:Metals / Q000032:analysis*
D008670:Metals