title: Homologous and heterologous protection after single intranasal administration of live attenuated recombinant Bordetella pertussis.
abstract: While single-dose mucosal immunization is best achieved by the use of attenuated live microorganisms, attenuation generally results in decreased immunogenicity. We attenuated Bordetella pertussis by the deletion of the pertussis toxin gene. A single intranasal administration of this strain protected against subsequent challenge as well as did the parent strain and better than immunization with commercial vaccine. Unexpectedly, this attenuation resulted in increased immunogenicity against the protective antigen filamentous hemagglutinin (FHA). In addition, immunogenicity was also enhanced against the Schistosoma mansoni Sm28GST genetically fused to FHA, resulting in protection against the parasite, as characterized by a reduction in worm burden and egg charge, after a single intranasal administration. Thus, attenuated recombinant B. pertussis strains are promising vectors for the simultaneous protection against pertussis and heterologous diseases by a single intranasal administration.
authors.affiliation: INSERM U167, Pasteur Institute of Lille, France.
authors.forename: N
authors.initials: N
authors.lastname: Mielcarek
authors.forename: G
authors.initials: G
authors.lastname: Riveau
authors.forename: F
authors.initials: F
authors.lastname: Remoué
authors.forename: R
authors.initials: R
authors.lastname: Antoine
authors.forename: A
authors.initials: A
authors.lastname: Capron
authors.forename: C
authors.initials: C
authors.lastname: Locht
mesh_terms: D018829:Adhesins, Bacterial / Q000235:genetics / Q000494:pharmacology; D000281:Administration, Intranasal; D000818:Animals; D000917:Antibody Formation / Q000276:immunology; D000942:Antigens, Bacterial / Q000235:genetics / Q000494:pharmacology; D001428:Bacterial Vaccines / Q000008:administration & dosage / Q000276:immunology / Q000494:pharmacology*; D001886:Bordetella pertussis / Q000235:genetics* / Q000276:immunology*; D005260:Female; D006388:Hemagglutinins / Q000235:genetics / Q000494:pharmacology; D051379:Mice; D037342:Pertussis Toxin; D012550:Schistosoma mansoni / Q000187:drug effects / Q000235:genetics; D012555:Schistosomiasis mansoni / Q000276:immunology / Q000517:prevention & control*; D010566:Virulence Factors, Bordetella / Q000276:immunology*; D014917:Whooping Cough / Q000276:immunology / Q000517:prevention & control*
chemical_list: D018829:Adhesins, Bacterial; D000942:Antigens, Bacterial; D001428:Bacterial Vaccines; D006388:Hemagglutinins; D010566:Virulence Factors, Bordetella; C065273:filamentous hemagglutinin adhesin, Bordetella pertussis; D037342:Pertussis Toxin