Glucose and fatty acid oxidation by the in situ dog heart during experimental cooling and rewarming.
BACKGROUND
Reduced myocardial function after hypothermia may be metabolic in origin, but the relationship between myocardial metabolism and the various components of hypothermia-mediated dysfunction has not been thoroughly investigated.


METHODS
In the present study we measured myocardial uptake and oxidation of glucose and oleate in mongrel dogs undergoing cooling to 25 degrees C followed by rewarming to 37 degrees C, using radiolabeled substrates.


RESULTS
Segment work index declined from 39.3 +/- 5.1 to 15.1 +/- 2.4 mm Hg in response to cooling from 37 degrees to 25 degrees C and did not recover completely on rewarming (27.2 +/- 4.2 mm Hg, p < 0.05). Oleate uptake declined from 3,251 +/- 619 to 1,043 +/- 356 nmol.min-1.100 g-1 (p < 0.05) when the dogs were cooled from 37 degrees to 25 degrees C. Simultaneously, oxidation rate fell from 1,089 +/- 158 to 354 +/- 83 nmol.min-1.100 g-1 (p < 0.05). On rewarming, oleate uptake was restored to prehypothermic values, whereas its rate of oxidation remained depressed (480 +/- 129 nmol.min-1.100 g-1; p < 0.05). Uptake and oxidation of glucose also declined significantly during cooling. However, both uptake and oxidation of glucose recovered fully on rewarming.


CONCLUSIONS
The results of the present study demonstrate a reduced capacity to oxidize fatty acids by the myocardium during rewarming after hypothermia.
Steigen
T K
TK
Department of Medical Physiology, University of Tromsø, Norway.
Tveita
T
T
Hevrøy
O
O
Andreasen
T V
TV
Larsen
T S
TS
D000818:Animals; D001786:Blood Glucose / Q000032:analysis; D002250:Carbon Radioisotopes; D003326:Coronary Circulation / Q000502:physiology; D004285:Dogs; D005230:Fatty Acids, Nonesterified / Q000097:blood; D005947:Glucose / Q000378:metabolism* / Q000493:pharmacokinetics; D006339:Heart Rate / Q000502:physiology; D007036:Hypothermia, Induced; D007773:Lactates / Q000097:blood; D009200:Myocardial Contraction / Q000502:physiology; D009206:Myocardium / Q000378:metabolism*; D019301:Oleic Acid / Q000378:metabolism* / Q000493:pharmacokinetics; D010084:Oxidation-Reduction; D019275:Radiopharmaceuticals; D017694:Rewarming; D014316:Tritium; D016277:Ventricular Function, Left / Q000502:physiology; D017725:Ventricular Pressure / Q000502:physiology
D001786:Blood Glucose; D002250:Carbon Radioisotopes; D005230:Fatty Acids, Nonesterified; D007773:Lactates; D019275:Radiopharmaceuticals; D014316:Tritium; D019301:Oleic Acid; D005947:Glucose