[Observation of the invasion of retinoblastoma cells to the corneal epithelial cells and keratocytes in culture].
OBJECTIVE
To study the invasive ability of retinoblastoma cells into the corneal epithelial cells and keratocytes in culture.


METHODS
SO-Rb50 cells of retinoblastoma, corneal epithelial cells and keratocytes were co-cultured. The morphological changes of SO-Rb50 cells adherent to the corneal epithelial cells and keratocytes were observed. The distribution of proteoglycans in the SO-Rb50 cells, corneal cells and keratocytes was analyzed by histochemical method and electron microscopy.


RESULTS
SO-Rb50 cells could adhere to the keratocytes, but did not adhere to the corneal epithelial cells. There are common hyaluronidase-resistant and chondroitinase-resistant proteoglycans on the surface of SO-Rb50 cells and corneal epithelial cells. Chondroitin is present on the surface of keratocytes.


CONCLUSIONS
It is considered that the cell surface proteoglycans may play an important role in mediating SO-Rb50 tumor cell adhesion and non-adhesion to normal cells.
Li
Y
Y
National Ophthalmological Laboratory, Zhongshan Ophthalmic Center, Sun Yat-sen University of Medical Sciences, Guangzhou.
Feng
G
G
Yi
Y
Y
D000328:Adult; D002478:Cells, Cultured; D018920:Coculture Techniques; D019573:Epithelium, Corneal / Q000166:cytology*; D006801:Humans; D009361:Neoplasm Invasiveness; D011509:Proteoglycans / Q000378:metabolism; D019572:Retinal Neoplasms / Q000378:metabolism / Q000473:pathology*; D012175:Retinoblastoma / Q000378:metabolism / Q000473:pathology*; D014407:Tumor Cells, Cultured
D011509:Proteoglycans