Dopamine-induced recruitment of dopamine D1 receptors to the plasma membrane.
The recruitment of G protein-coupled receptors from the cytoplasm to the plasma membrane generally is believed to be a constitutive process. We show here by the use of both confocal microscopy and subcellular fractionation that, for at least one such receptor, this recruitment is regulated and not constitutive. Cells from a proximal tubular-like cell line, LLCPK1 cells, were incubated with either a D1 agonist, a dopamine precursor, or an inhibitor of dopamine metabolism to increase dopamine availability in the cell. Each of the three procedures led to a rapid translocation of dopamine D1 receptors from the cytosol to the plasma membrane.
Brismar
H
H
Department of Woman and Child Health, Karolinska Institutet, Astrid Lindgrens Children's Hospital, 171 76 Stockholm, Sweden.
Asghar
M
M
Carey
R M
RM
Greengard
P
P
Aperia
A
A
D001552:Benzazepines / Q000494:pharmacology; D002396:Catechols / Q000494:pharmacology; D002460:Cell Line; D002462:Cell Membrane / Q000187:drug effects / Q000378:metabolism; D005576:Colforsin / Q000494:pharmacology; D004298:Dopamine / Q000494:pharmacology*; D018491:Dopamine Agonists / Q000494:pharmacology; D018492:Dopamine Antagonists / Q000494:pharmacology; D004791:Enzyme Inhibitors; D018818:Fenoldopam / Q000494:pharmacology; D019085:Fluorescent Antibody Technique, Direct; D006801:Humans; D007687:Kidney Tubules, Proximal / Q000187:drug effects / Q000378:metabolism; D018613:Microscopy, Confocal; D010422:Pentanones / Q000494:pharmacology; D017447:Receptors, Dopamine D1 / Q000378:metabolism*; D000254:Sodium-Potassium-Exchanging ATPase / Q000378:metabolism
D001552:Benzazepines; D002396:Catechols; D018491:Dopamine Agonists; D018492:Dopamine Antagonists; D004791:Enzyme Inhibitors; D010422:Pentanones; D017447:Receptors, Dopamine D1; D005576:Colforsin; C057364:nitecapone; D000254:Sodium-Potassium-Exchanging ATPase; D018818:Fenoldopam; D004298:Dopamine