Characterization of canine pericardial fluid endothelin-1 levels.
Recently, extremely high concentrations of endothelin-1 (ET-1) were detected in the pericardial fluid of patients undergoing cardiac surgery. This study was designed to characterize ET-1 levels in plasma and pericardial fluid of dogs. Plasma and pericardial fluid samples were extracted using SepPak C18 cartridges and ET-1 levels were measured by a specific radioimmunoassay. Pericardial fluid ET-1 levels (739 +/- 81 pg/ml; n = 10) were significantly higher than respective plasma levels (22.4 +/- 4.0 pg/ml; p < 0.05). The mean pericardial fluid: plasma ratio of ir-ET-1 concentration was 54.7 +/- 16.8. In HPLC analysis, the total ET-1-like immunoreactivity of pericardial fluid co-eluted with ET-1 standard. In anesthetized dogs (n = 6), disappearance of [125I]ET-1 was sixfold slower in the pericardial space than in plasma. Our results show that high concentrations of ir-ET-1 can be found in canine pericardial fluid. Slow elimination of ET-1 from the pericardial fluid compartment may contribute to high peptide levels.
Szokodi
I
I
Department of Cardiovascular Surgery, Semmelweis University Medical School, Budapest, Hungary.
Horkay
F
F
Kiss
P
P
Selmeci
L
L
Horváth
I
I
Vuolteenaho
O
O
Ruskoaho
H
H
Juhász-Nagy
A
A
Tóth
M
M
D000818:Animals; D001826:Body Fluids / Q000378:metabolism; D002851:Chromatography, High Pressure Liquid; D004285:Dogs; D019332:Endothelin-1 / Q000097:blood / Q000378:metabolism*; D006207:Half-Life; D007457:Iodine Radioisotopes; D010496:Pericardium / Q000378:metabolism*; D011863:Radioimmunoassay
D019332:Endothelin-1; D007457:Iodine Radioisotopes