The immunodominant Brugia malayi paramyosin as a marker of current infection with Wuchereria bancrofti adult worms.
The full-length cDNA sequence encoding Brugia malayi L3 paramyosin has been isolated by immunoscreening a cDNA library with a mouse antiserum raised against Wuchereria bancrofti L3 infective larvae. A recombinant truncated form of paramyosin was expressed as a glutathione S-transferase fusion protein and used to evaluate humoral responses of adults from a W. bancrofti-endemic area in French Polynesia according to their parasitological status. Immunoglobulin G4 (IgG4) preferentially bound to paramyosin in W. bancrofti-parasitized individuals, in contrast to unparasitized individuals, who harbored neither microfilaria nor Og4C3 adult worm circulating antigen. Reduction of the anti-paramyosin IgG4 titer following combined chemotherapy with diethylcarbamazine and ivermectin was significantly correlated with a reduction in the adult worm burden. This indicates that the presence of paramyosin-reactive IgG4 is associated with the presence of parasites and that reduction can be used as an immunological marker for W. bancrofti clearance.
Langy
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S
Institut Territorial de Recherches Médicales Louis Malardé, Papeete, Tahiti, French Polynesia.
Plichart
C
C
Luquiaud
P
P
Williams
S A
SA
Nicolas
L
L
D000328:Adult; D000818:Animals; D000909:Antibodies, Helminth / Q000097:blood; D000947:Antigens, Helminth / Q000235:genetics / Q000276:immunology; D015415:Biomarkers; D017178:Brugia malayi / Q000235:genetics / Q000276:immunology*; D018076:DNA, Complementary / Q000235:genetics; D004049:Diethylcarbamazine / Q000627:therapeutic use; D005368:Filariasis / Q000175:diagnosis* / Q000453:epidemiology; D005369:Filaricides / Q000627:therapeutic use; D005982:Glutathione Transferase / Q000235:genetics; D015801:Helminth Proteins / Q000235:genetics / Q000276:immunology; D006801:Humans; D016056:Immunodominant Epitopes; D007559:Ivermectin / Q000627:therapeutic use; D008969:Molecular Sequence Data; D010446:Peptide Fragments / Q000235:genetics / Q000276:immunology; D011114:Polynesia / Q000453:epidemiology; D011993:Recombinant Fusion Proteins / Q000276:immunology; D016896:Treatment Outcome; D014335:Tropomyosin / Q000235:genetics / Q000276:immunology; D014958:Wuchereria bancrofti / Q000302:isolation & purification*
D000909:Antibodies, Helminth; D000947:Antigens, Helminth; D015415:Biomarkers; D018076:DNA, Complementary; D005369:Filaricides; D015801:Helminth Proteins; D016056:Immunodominant Epitopes; D010446:Peptide Fragments; D011993:Recombinant Fusion Proteins; D014335:Tropomyosin; D007559:Ivermectin; D005982:Glutathione Transferase; D004049:Diethylcarbamazine