title: BRCA-associated breast cancer: absence of a characteristic immunophenotype.
abstract: To characterize the biological features of breast cancer associated with germ-line mutations in BRCA1 and BRCA2, invasive tumors were studied from 58 Jewish women ascertained through studies of early-onset breast cancer. All women were tested for the BRCA1 founder mutations 187delAG (commonly known as 185delAG) and 5385insC (commonly known as 5382insC) and the BRCA2 founder mutation 6174delT. Mutations were detected in 17 of 58 (29.3%) women. Comparing BRCA-associated breast cancers (BABCs) to cases arising in women without founder mutations, no differences were noted in tumor size, tumor stage, or frequency of axillary nodal involvement. Infiltrating ductal carcinoma was the predominant histological type in both groups. BABCs were significantly more likely to be of histological grade III (100 versus 63%; P = 0.04), estrogen receptor negative (75 versus 35%; P = 0.004), and HER2/neu negative (87 versus 58%; P = 0.04). An associated intraductal component was present in 59% of BABCs and 76% of cancers not associated with mutations (P = not significant). A high Ki-67 labeling index was more commonly observed in BABCs than in cases without mutations (83 versus 48%; P = 0.09). There were no differences between the two groups in the frequency of expression of epidermal growth factor receptor, cathepsin D, bcl-2, p27, p53, or cyclin D. There were no significant differences in relapse-free or overall survival. These observations suggest that breast cancers arising in Jewish women with germ-line BRCA founder mutations have a greater proliferative potential than cancers in women without such mutations. Additional studies of BABC are required to determine the nature and implications of additional genetic abnormalities occurring in these tumors.
authors.affiliation: Department of Human Genetics, Memorial Sloan-Kettering Cancer Center, New York, New York 10021, USA. robsonm@mskcc.org
authors.forename: M
authors.initials: M
authors.lastname: Robson
authors.forename: P
authors.initials: P
authors.lastname: Rajan
authors.forename: P P
authors.initials: PP
authors.lastname: Rosen
authors.forename: T
authors.initials: T
authors.lastname: Gilewski
authors.forename: Y
authors.initials: Y
authors.lastname: Hirschaut
authors.forename: P
authors.initials: P
authors.lastname: Pressman
authors.forename: B
authors.initials: B
authors.lastname: Haas
authors.forename: L
authors.initials: L
authors.lastname: Norton
authors.forename: K
authors.initials: K
authors.lastname: Offit
mesh_terms: D000328:Adult; D019313:BRCA1 Protein / Q000235:genetics* / Q000378:metabolism; D024682:BRCA2 Protein; D001943:Breast Neoplasms / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D002278:Carcinoma in Situ / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D018270:Carcinoma, Ductal, Breast / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D018275:Carcinoma, Lobular / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D015331:Cohort Studies; D005260:Female; D005787:Gene Frequency; D019398:Genes, BRCA1 / Q000235:genetics; D018095:Germ-Line Mutation; D006801:Humans; D007150:Immunohistochemistry; D016130:Immunophenotyping; D009363:Neoplasm Proteins / Q000235:genetics* / Q000378:metabolism; D014157:Transcription Factors / Q000235:genetics* / Q000378:metabolism
chemical_list: D019313:BRCA1 Protein; D024682:BRCA2 Protein; D009363:Neoplasm Proteins; D014157:Transcription Factors