Purification and characterization of CD38/ADP-ribosyl cyclase from rat lung.
Purification and characterization of CD38/ADP-ribosyl cyclase in the rat lung tissue was performed with microsomes solubilized in Triton X-100 and the ADP-ribosyl cyclase was then purified using sequential column chromatography. Partially purified rat lung ADP-ribosyl cyclase was analyzed by immunoblotting using an antibody raised against a recombinant rat CD38 and showed the presence of monomer (42 kDa) and dimer (85 kDa) under non-reducing conditions but under reducing conditions, only the monomer was detected. Both the monomer and dimer could be eluted out in a stable manner from SDS-PAGE and the enzymatic activity was retained by the two different forms of CD38/ADP-ribosyl cyclase. Immunohistochemical staining showed the presence of CD38 on the bronchial epithelium and the alveoli.
Khoo
K M
KM
Department of Biochemistry, Faculty of Medicine, National University of Singapore, Singapore.
Chang
C F
CF
D036541:ADP-ribosyl Cyclase; D051997:ADP-ribosyl Cyclase 1; D000818:Animals; D015703:Antigens, CD; D000943:Antigens, Differentiation / Q000302:isolation & purification* / Q000378:metabolism*; D001980:Bronchi / Q000201:enzymology; D002467:Cell Nucleus / Q000201:enzymology; D002498:Centrifugation; D003593:Cytoplasm / Q000201:enzymology; D004591:Electrophoresis, Polyacrylamide Gel; D004847:Epithelial Cells / Q000201:enzymology; D007150:Immunohistochemistry; D008168:Lung / Q000201:enzymology*; D008562:Membrane Glycoproteins; D008861:Microsomes / Q000201:enzymology; D009244:NAD+ Nucleosidase / Q000302:isolation & purification* / Q000378:metabolism*; D011650:Pulmonary Alveoli / Q000201:enzymology; D011817:Rabbits; D051381:Rats
D015703:Antigens, CD; D000943:Antigens, Differentiation; D008562:Membrane Glycoproteins; D036541:ADP-ribosyl Cyclase; C498156:Cd38 protein, rat; D009244:NAD+ Nucleosidase; D051997:ADP-ribosyl Cyclase 1