Accurate measurement of cholecystokinin in plasma.
Shortage of reliable plasma assays has hampered studies of cholecystokinin (CCK). The assay problems are low plasma concentrations, extensive molecular heterogeneity, and close homology of CCK to gastrin, which circulates in higher concentrations. To develop an accurate CCK RIA, antibodies were raised in rabbits, guinea pigs, and mice in titers from 200 to 4000000. The specificity of the antisera was tested with homologous peptides, and tissue and plasma extracts. Rabbit 92128 produced antibodies in high titer (> or =500000) with sufficient avidity (K(o)eff > or = 10(12) mol(-1)) and the desired specificity. The antiserum binds the bioactive forms of CCK with equimolar potency and displays no reactivity with gastrin. CCK concentrations in plasma from healthy humans rose from 1.13 +/- 0.10 pmol/L (mean +/- SE, n = 26) to 4.92 +/- 0.34 pmol/L after a mixed meal. Chromatography of human plasma revealed traces of CCK-58, a predominance of CCK-33 and CCK-22, and moderate amounts of CCK-8. The results show that it is possible to produce specific CCK-antisera using a sulfated CCK-12 analog.
Rehfeld
J F
JF
Dept. of Clinical Biochemistry, Rigshospitalet, Copenhagen, Denmark. rehfeld@rh.dk
D000328:Adult; D000818:Animals; D000906:Antibodies / Q000276:immunology; D000918:Antibody Specificity; D002766:Cholecystokinin / Q000032:analysis / Q000097:blood* / Q000276:immunology; D003429:Cross Reactions; D005260:Female; D005755:Gastrins / Q000097:blood; D006168:Guinea Pigs; D006801:Humans; D007114:Immunization; D007413:Intestinal Mucosa / Q000737:chemistry; D008297:Male; D051379:Mice; D010446:Peptide Fragments / Q000032:analysis; D010649:Phenylalanine / Q000031:analogs & derivatives / Q000276:immunology; D011817:Rabbits; D011863:Radioimmunoassay / Q000379:methods; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013552:Swine; D014443:Tyrosine / Q000031:analogs & derivatives / Q000276:immunology
D000906:Antibodies; D005755:Gastrins; D010446:Peptide Fragments; C005662:tyrosine O-sulfate; D014443:Tyrosine; D010649:Phenylalanine; C020457:phenylalanine amide; D002766:Cholecystokinin