title: Transfer of plasmid pTO1 from Escherichia coli to various representatives of the order Actinomycetales by intergeneric conjugation.
abstract: Plasmid pTO1 containing the oriT fragment from RK2, the Escherichia coli replication function from pBR322, and a DNA fragment of actinophage phi C31 with the attachment site was transferred from E. coli S17-1 to strains of the genera Actinomadura, Arthrobacter, Micromonospora, Nocardia, Rhodococcus, and to 16 strains of the genus Streptomyces. The frequency of conjugant formation was 1 x 10(-3)-1 x 10(-5) depending on the strain. Hybridization experiments demonstrated that plasmid pTO1 integrates into chromosomes of a number of the recipient strains examined.
authors.affiliation: State Scientific Centre of the Russian Federation, GNIIGENETIKA, Moscow, Russia. LOMOV@VNIGEN.MSK.SU
authors.forename: T
authors.initials: T
authors.lastname: Voeykova
authors.forename: L
authors.initials: L
authors.lastname: Emelyanova
authors.forename: V
authors.initials: V
authors.lastname: Tabakov
authors.forename: N
authors.initials: N
authors.lastname: Mkrtumyan
mesh_terms: D000192:Actinomycetales / Q000235:genetics*; D001287:Attachment Sites, Microbiological; D001435:Bacteriophages; D002876:Chromosomes, Bacterial / Q000235:genetics; D003227:Conjugation, Genetic / Q000235:genetics*; D004926:Escherichia coli / Q000235:genetics*; D010957:Plasmids / Q000235:genetics*; D013302:Streptomyces / Q000235:genetics / Q000821:virology