Prostaglandin-D-synthase (beta-trace protein) levels in rat cerebrospinal fluid.
The precise role of prostaglandin-D-synthase (beta-trace protein), the major constituent of cerebrospinal fluid, is unclear. In the present study, a sensitive and highly specific fluoroimmunoassay was developed. The measurement of the enzyme levels in rat CSF revealed a developmental change in the CSF levels with the highest value of 66 +/- 8 microg/ml at 7 days after birth. No significant difference in the levels was seen between different times of day. Subcutaneous injections of all-trans retinoic acid caused a dramatic decrease in the protein levels in a dose- and time-dependent manner. These findings may raise the possibility that prostaglandin-D-synthase in CSF is involved in retinoic acid action on the brain.
Kabeya
K
K
Department of Molecular Physiology, Institute for Molecular and Cellular Regulation, Gunma University, Maebashi, Japan.
Ishikawa
K
K
Katakai
K
K
Watanabe
M
M
Ohe
Y
Y
Wakabayashi
K
K
Mori
M
M
D000375:Aging / Q000134:cerebrospinal fluid; D000818:Animals; D001609:Beta-Globulins / Q000134:cerebrospinal fluid*; D015153:Blotting, Western; D019085:Fluorescent Antibody Technique, Direct; D019746:Intramolecular Oxidoreductases; D054834:Lipocalins; D008297:Male; D051381:Rats; D017208:Rats, Wistar; D013256:Steroids / Q000494:pharmacology; D013963:Thyroid Hormones / Q000494:pharmacology; D013997:Time Factors; D014212:Tretinoin / Q000494:pharmacology
D001609:Beta-Globulins; D054834:Lipocalins; D013256:Steroids; D013963:Thyroid Hormones; D014212:Tretinoin; D019746:Intramolecular Oxidoreductases; C022466:prostaglandin R2 D-isomerase