title: Antibacterial activity of calcium hydroxide-containing endodontic sealers on Enterococcus faecalis in vitro.
abstract: Antimicrobial activity of endodontic sealers is an important factor in the prognosis of root canal treatment. The aim of this study was to analyse the antimicrobial activity of calcium hydroxide-containing endodontic sealers, Sealapex (SA) and CRCS compared to a ZOE-containing sealer, Roth's cement. The sealers were mixed and placed on the side wall of microtitre plate wells. A 10 microL suspension of Enterococcus faecalis was added to the surface of the sealers 1 h, 24 h, or 7 days after mixture. Bacteria were allowed to come into contact with the sealers for 1 h at 37 degrees C. Fresh media was then added and bacterial growth was measured every 30 min for 16 h. The results showed that in 1-hour-old mixture, CRCS and Roth's cement had a significantly better antimicrobial effect than SA. In 24-hour-old mixtures, ZOE-based sealer showed a more potent antimicrobial activity than calcium hydroxide-containing sealers, whereas SA showed a significantly better antimicrobial effect in the 7-day-old mixture. The antimicrobial activity of each tested sealer changes differently with the time interval between mixing and testing, suggesting different physicochemical properties and potentially diverse clinical applications.
authors.affiliation: Department of Endodontology, The Maurice and Gabriela Goldshleger School of Dental Medicine, Tel Aviv University, Israel.
authors.forename: Z
authors.initials: Z
authors.lastname: Fuss
authors.forename: E I
authors.initials: EI
authors.lastname: Weiss
authors.forename: M
authors.initials: M
authors.lastname: Shalhav
mesh_terms: D002126:Calcium Hydroxide / Q000494:pharmacology*; D003786:Dental Pulp Cavity / Q000382:microbiology; D013293:Enterococcus faecalis / Q000187:drug effects* / Q000302:isolation & purification; D006801:Humans; D008826:Microbial Sensitivity Tests / Q000379:methods; D012387:Root Canal Filling Materials / Q000494:pharmacology*; D012459:Salicylates / Q000494:pharmacology*; D013997:Time Factors; D015034:Zinc Oxide / Q000494:pharmacology; D015035:Zinc Oxide-Eugenol Cement / Q000494:pharmacology
chemical_list: D012387:Root Canal Filling Materials; D012459:Salicylates; D015035:Zinc Oxide-Eugenol Cement; C055601:Sealapex; C075563:Calcibiotic Root Canal Sealer; D002126:Calcium Hydroxide; D015034:Zinc Oxide