Soluble IL-6 receptor release by PMNs and PBMC in patients with oral cavity cancer.
It is known that the main source of cytokine and soluble cytokine receptors are mononuclear cells in the peripheral blood. Data including the release of soluble mediators by polymorphonuclear cells (PMNs) are controversial. In these examinations using an in vitro model, we studied the ability of spontaneous and LPS-stimulated PMNs to the release of soluble IL-6 receptor (IL-6sR) in oral cavity cancer patients. Obtained results were compared to the release of IL-6sR by a peripheral blood mononuclear cells (PBMC) and the serum levels. The concentrations of IL-6sR in the culture supernatants of cells and in the serum were tested by ELISA. The culture supernatants of spontaneous and LPS-stimulated PMNs derived from oral cavity cancer patients contained an insignificant lower concentrations of IL-6sR than those from healthy subjects. The concentrations of IL-6sR released by PBMC were higher than those released by PMNs in both control and patient groups. There was correlation between the amounts of IL-6sR in the culture supernatants of spontaneous PMNs and PBMC. The altered ability of PMNs and PBMC to the release of IL-6sR may influence the immune response of host to tumor mediated by IL-6.
Jabłońska
E
E
Department of Immunopathology, Medical Academy of Białystok.
Piotrowski
L
L
Grabowska
Z
Z
D000328:Adult; D000368:Aged; D002294:Carcinoma, Squamous Cell / Q000097:blood / Q000276:immunology / Q000473:pathology; D002478:Cells, Cultured; D017077:Culture Media, Conditioned; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006801:Humans; D007958:Leukocyte Count; D007963:Leukocytes, Mononuclear / Q000187:drug effects / Q000378:metabolism*; D008070:Lipopolysaccharides / Q000494:pharmacology; D008297:Male; D008875:Middle Aged; D009062:Mouth Neoplasms / Q000097:blood / Q000276:immunology* / Q000473:pathology; D009504:Neutrophils / Q000187:drug effects / Q000378:metabolism*; D019947:Receptors, Interleukin-6 / Q000097:blood / Q000187:drug effects / Q000378:metabolism*
D017077:Culture Media, Conditioned; D008070:Lipopolysaccharides; D019947:Receptors, Interleukin-6