[Glutamine dipeptide attenuate mucosal atrophic changes and preservation of gut barrier function following 5-FU intervention].
Traditional parenteral nutrition (PN) and chemotherapy may lead to changes mucosal morphology and gut barrier function. To investigate the effects of alanyl-glutamine on intestinal mucosal morphology and gut barrier function in PN-fed rats challenged with 5-FU male Wistar rats were central catheterized and randomily divided into two groups: PN group (n = 10) that received traditional parenteral nutrition solution only, and Ala-Gln group (n = 10) that received glutamine dipeptide enriched nutritional solution (3% Ala-Gln). The rats were maintained on their respective diets for 7 days. 5-FU (75 mg/kg) was injected intraperitoneally at 8 am on day 4. All rats were gavaged with lactulose (100 mg) and mannitol (50 mg) in 2ml before and three days after administration of 5-FU. Ala-Gln group maintained serum glutamine concentration, intestinal mucosal morphology. While bacterial translocation rates in ala-Gln group was 30%, in PN group was 90% (P < 0.05). Similar intestinal permeability was observed on day 3 in both groups. The control group had a significantly increased intestinal permeability on day 7 (P < 0.05), while Ala-Gln group maintained the intestinal permeability. It was suggested that alanyl-glutamine maintained intestinal morphology and gut barrier function in PN-fed rats challenged with 5-FU.
Bai
M
M
Peking Union Medical College Hospital, Chinese Academy of Medical Sciences, Beijing.
Jiang
Z
Z
Liu
Y
Y
D000818:Animals; D001284:Atrophy / Q000517:prevention & control; D018988:Bacterial Translocation / Q000187:drug effects; D004151:Dipeptides / Q000494:pharmacology*; D005472:Fluorouracil / Q000009:adverse effects; D007413:Intestinal Mucosa / Q000473:pathology*; D008297:Male; D010288:Parenteral Nutrition / Q000009:adverse effects; D010539:Permeability; D011897:Random Allocation; D051381:Rats; D017208:Rats, Wistar
D004151:Dipeptides; D005472:Fluorouracil; C054122:alanylglutamine