Differentiation of a porcine reproductive and respiratory syndrome virus vaccine strain from North American field strains by restriction fragment length polymorphism analysis of ORF 5.
The suitability of restriction fragment length polymorphism (RFLP) analysis for differentiating a porcine reproductive and respiratory syndrome virus (PRRSV) vaccine strain from other North American field strains was investigated. Open reading frame 5 nucleotide sequence data of the vaccine virus, its parent strain VR-2332, and 22 other strains of PRRSV included in this study indicated that 3 restriction enzyme gel patterns characterize the vaccine virus and the parent strain genotype. The combined 3 RFLP patterns differentiate the vaccine and parent virus from other PRRSV strains. This test will be a valuable tool in epidemiologic studies and will be useful in identifying individual strains in cases of multistrain PRRSV infections.
Wesley
R D
RD
Virology Swine Research Unit, National Animal Disease Center, USDA, Agricultural Research Service, Ames, IA 50010, USA.
Mengeling
W L
WL
Lager
K M
KM
Clouser
D F
DF
Landgraf
J G
JG
Frey
M L
ML
D000818:Animals; D001483:Base Sequence; D003062:Codon; D004262:DNA Restriction Enzymes; D009656:North America; D016366:Open Reading Frames; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length; D019318:Porcine Reproductive and Respiratory Syndrome / Q000276:immunology* / Q000517:prevention & control; D019316:Porcine respiratory and reproductive syndrome virus / Q000145:classification / Q000235:genetics* / Q000276:immunology*; D012367:RNA, Viral / Q000032:analysis; D013552:Swine; D014765:Viral Vaccines
D003062:Codon; D012367:RNA, Viral; D014765:Viral Vaccines; D004262:DNA Restriction Enzymes