Some parameters influencing immunoassay of human and horse myoglobins.
It was noted that human and horse sera as well as human heart and skeletal muscle homogenates or extracts distinctly decrease immunoassays of purified myoglobins. The assays of homogenate and extract myoglobins could be many times increased by precipitation certain proteins with concentrated ammonium sulfate or sodium chloride. Also in homogenates and extracts incubated for several days increased assays of myoglobins were noted. The obtained results indicate that both myoglobins occur in complex with other tissue component(s).
Kochanowska
I E
IE
Biochemical Laboratory, Institute of Immunology and Experimental Therapy, Polish Academy of Sciences, Wrocław, Poland.
Kuropatwa
M
M
Szewczuk
A
A
D000818:Animals; D016477:Artifacts; D001798:Blood Proteins / Q000378:metabolism; D002458:Cell Fractionation; D011232:Chemical Precipitation; D004797:Enzyme-Linked Immunosorbent Assay; D006736:Horses / Q000378:metabolism*; D006801:Humans; D018482:Muscle, Skeletal / Q000737:chemistry*; D009206:Myocardium / Q000737:chemistry*; D009211:Myoglobin / Q000032:analysis*; D009928:Organ Specificity; D011485:Protein Binding; D012680:Sensitivity and Specificity; D013045:Species Specificity; D013048:Specimen Handling; D013696:Temperature
D001798:Blood Proteins; D009211:Myoglobin