Effect of enamel microabrasion on bacterial colonization.
OBJECTIVE
To determine if microabraded surfaces inhibit Streptococcus mutans colonization.


METHODS
Ten extracted molars were obtained and sectioned in half mesio-distally. The mesial side was treated with microabrasion (PREMA Compound). The distal surface was designated as the control site. A plastic tube was secured on each surface with resin composite, exposing a surface of 13.2 mm 2. S. mutans 10,499 was cultured in TSB-YE supplemented with 5% sucrose and 2.5% glucose was added to each secured site in addition to 50 microliters of cell suspension. Samples were incubated in a 5% CO2 chamber for 7 days at 37 degrees C. Samples were taken from each site, incubated with dextranase (75 micrograms 1 ml), sonicated, and vortexed to disrupt cellular aggregates as much as possible. Samples were then plated on Mitis-Salivarius agar using a spiral plating system. Enumeration of numbers of colonizing bacteria was achieved following standard spiral plating system methodology.


RESULTS
The mean for the microabraded group was 1.12 x 10(2) cfu/tooth and for the control it was 1.15 x 10(4). Analyzing the data revealed a significant difference (P = 0.0188).
Segura
A
A
University of Iowa Hospitals and Clinics, Iowa City 52242, USA.
Donly
K J
KJ
Wefel
J S
JS
Drake
D
D
D015169:Colony Count, Microbial; D045424:Complex Mixtures; D003743:Dental Enamel / Q000382:microbiology*; D003773:Dental Plaque / Q000382:microbiology; D003802:Dentifrices; D019243:Enamel Microabrasion / Q000379:methods; D006801:Humans; D066298:In Vitro Techniques; D008963:Molar; D013295:Streptococcus mutans / Q000254:growth & development / Q000302:isolation & purification; D013499:Surface Properties
D045424:Complex Mixtures; D003802:Dentifrices; C070504:Prema