Reproductive toxicity and tissue concentrations of 3,3',4,4'-tetrachlorobiphenyl (PCB 77) in male adult rats.
1 The aim of this study was to ascertain the reproductive effects of PCB 77 on adult male rats and to determine its concentration in the liver and testis. Adult male rats (n = 15/group) were treated subcutaneously with a single dose of 18 mg/kg bw (PC18) or with 60 mg/kg bw (PC60). The substance was dissolved in a 10 ml volume of peanut oil/kg. Control rats received the same volume of the vehicle. The reproductive effects as well as the concentration of PCB 77 in the liver and testis were investigated 1, 4 and 8 weeks after treatment. 2 In both groups, the daily sperm production (DSP; x10(6)) remained permanently reduced in the PC18 as well as in the PC60 groups throughout the entire investigation period (DSP week 8: control: 31 +/- 7; PC18: 22 +/- 5; PC60: 20 +/- 7). The sperm number (x10(6)) per cauda epididymis was affected only at the 1st and 4th week after treatment (control week 1: 211 +/- 67; PC18 week 1: 135 +/- 62; PC60 week 1: 142 +/- 49). Moreover, a significant increase in the percentage of abnormal sperm was observed 4 weeks following treatment in the PC18 and PC60 groups and 8 weeks after treatment in the PC60 group. Abnormal tails were the most frequent changes observed. 3 The relative testicular and prostata weights (g) were slightly increased in the PC60 group at the 1st and 4th week following treatment (testis weight: control/I: 0.46 +/- 0.02; PC60/I: 0.51 +/- 0.03). 4 The serum testosterone concentrations and effects on testis morphology were not reported. 5 The maximum concentration of PCB 77 was detected in the liver and testis 1 week after treatment. The concentration declined 4 weeks after treatment in both organs, but still a significant amount of PCB 77 was detectable in the liver as well as in the testis 8 weeks after treatment. 6 The results demonstrate that PCB 77 affects sperm variables when applied to adult rats and that the elimination of PCB 77 in the testis parallels that of the liver.
Faqi
A S
AS
Institut für Klinische Pharmakologie und Toxikologie, Freie Universität Berlin, Germany.
Dalsenter
P R
PR
Mathar
W
W
Heinrich-Hirsch
B
B
Chahoud
I
I
D000818:Animals; D004822:Epididymis / Q000187:drug effects / Q000473:pathology; D007279:Injections, Subcutaneous; D008099:Liver / Q000378:metabolism*; D008297:Male; D009929:Organ Size / Q000187:drug effects; D011078:Polychlorinated Biphenyls / Q000493:pharmacokinetics / Q000633:toxicity*; D011467:Prostate / Q000187:drug effects / Q000473:pathology; D051381:Rats; D012098:Reproduction / Q000187:drug effects*; D018570:Risk Assessment; D013076:Sperm Count / Q000187:drug effects; D013094:Spermatozoa / Q000187:drug effects / Q000473:pathology; D013737:Testis / Q000187:drug effects / Q000378:metabolism* / Q000473:pathology; D013739:Testosterone / Q000097:blood; D013997:Time Factors
D013739:Testosterone; D011078:Polychlorinated Biphenyls; C028451:3,4,3',4'-tetrachlorobiphenyl