Alcohol dehydrogenase (ADH) activity in the sera of patients during the course of viral hepatitis.
Total alcohol dehydrogenase activity in the sera of 77 patients in the course of viral hepatitis was determined by means of photometric method based on p-nitrosodimethylaniline reduction. Blood samples were taken 5 times at intervals of 7 to 9 day. We found that serum total ADH activity was higher at the onset of disease than that of the control group. The highest increase of activity was observed in the first week of hospitalisation, and exceeded the mean control value about 7 times. After that, the activity of ADH gradually decreased, and reached the value of the control group in the last period of the study. During five weeks of the study the total activity of ADH showed a good linear correlation with alanine and aspartate aminotransferases. We concluded that total alcohol dehydrogenase activity measured by photometric method increase in the course of viral hepatitis and correlate with the progress and treatment of disease measured by commonly accepted enzymatic markers of liver cell damage.
Chrostek
L
L
Department of Biochemical Diagnostics, Medical Academy of Białystok.
Szmitkowski
M
M
Panasiuk
A
A
D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000410:Alanine Transaminase / Q000097:blood; D000426:Alcohol Dehydrogenase / Q000097:blood* / Q000378:metabolism; D000469:Alkaline Phosphatase / Q000097:blood; D001219:Aspartate Aminotransferases / Q000097:blood; D018450:Disease Progression; D005260:Female; D005500:Follow-Up Studies; D006509:Hepatitis B / Q000097:blood / Q000201:enzymology* / Q000473:pathology; D006760:Hospitalization; D006801:Humans; D007770:L-Lactate Dehydrogenase / Q000097:blood; D008099:Liver / Q000201:enzymology / Q000473:pathology; D008297:Male; D008875:Middle Aged; D009603:Nitroso Compounds / Q000378:metabolism; D010084:Oxidation-Reduction; D010783:Photometry; D005723:gamma-Glutamyltransferase / Q000097:blood
D009603:Nitroso Compounds; C008651:4-nitrosodimethylaniline; D000426:Alcohol Dehydrogenase; D007770:L-Lactate Dehydrogenase; D005723:gamma-Glutamyltransferase; D001219:Aspartate Aminotransferases; D000410:Alanine Transaminase; D000469:Alkaline Phosphatase