[Quantitative analysis of prevention effect of tetrandrine on pancreatic islet beta cells injury in rats].
OBJECTIVE
To observe the preventive effect of tetrandrine (TET) on pancreatic islet beta cells damaged by alloxan in rats with HE, Gomori aldehyde fuchsine staining and immunocytochemistry.


METHODS
Animal diabetic models were prepared with alloxan (50 mg/kg i.v.). Pancreatic islet beta cells density (BD) in the section of HRP-SPA immunocytochemistry staining was determined with weibel square system.


RESULTS
The BD in experimental groups pretreated with TET (100 mg/kg) at 1.5 hours, 5 hours prior to alloxan injection increased from the control value of 13 +/- 4 to 62 +/- 9 and 65 +/- 7 (P < 0.001), which had no evident difference compared with the BV in normal control group; while the BV (22.5 +/- 7.7) in the pretreated group at 7 hours prior to alloxan injection also increased compared with the control group. But it was much lower than that in the normal control group. When the doses of TET decreased from 100 mg/kg to 50 mg/kg and 25 mg/kg, the BD declined from 62 +/- 9 to 45 +/- 5 and 38 +/- 4 (P < 0.01 and P < 0.001).


CONCLUSIONS
Tetrandrine can protect islet beta cells a from injury induced by alloxan.
Sun
G
G
Endocrinology Laboratory, Qingdao Medical College.
Qi
Y
Y
Pan
Q
Q
D000470:Alkaloids / Q000494:pharmacology*; D000818:Animals; D000975:Antioxidants / Q000494:pharmacology*; D044182:Benzylisoquinolines; D003921:Diabetes Mellitus, Experimental / Q000517:prevention & control*; D004305:Dose-Response Relationship, Drug; D005260:Female; D007515:Islets of Langerhans / Q000473:pathology*; D008297:Male; D051381:Rats; D017208:Rats, Wistar
D000470:Alkaloids; D000975:Antioxidants; D044182:Benzylisoquinolines; C009438:tetrandrine