Antigen presentation by B7-nonprofessional APC does not prevent responses to solid tumor cells.
The growth of tumors in vivo often is associated with immune suppression. In this report we tested whether the expression of a known antigen by tumor cells would inhibit the development of antitumor responses when the antigen was subsequently expressed in an immunogenic form. For this, we expressed a well-characterized surrogate tumor antigen, the nucleoprotein (NP) of the PR8 virus, in solid tumor cells. Although the NP+ tumor cells were not rejected in vivo and stimulated nondetectable CTL response in vitro, T cells from these mice differentiated into CTL following i.p. inoculation of PR8 virus and their tumors regressed. The results suggested that prior presentation of tumor antigens by tumor cells does not necessary preclude a response if the peptide is subsequently presented appropriately.
Salvadori
S
S
Department of Medicine, Mount Sinai School of Medicine, New York, New York 10029, USA.
Moran
T
T
Zier
K
K
D000818:Animals; D017951:Antigen Presentation / Q000276:immunology*; D000938:Antigen-Presenting Cells / Q000276:immunology*; D015703:Antigens, CD / Q000276:immunology*; D051940:B7-2 Antigen; D002460:Cell Line; D002642:Chick Embryo; D004285:Dogs; D006801:Humans; D007114:Immunization; D009980:Influenza A virus / Q000276:immunology; D007274:Injections, Intraperitoneal; D008562:Membrane Glycoproteins / Q000276:immunology*; D051379:Mice; D008807:Mice, Inbred BALB C; D009374:Neoplasms, Experimental / Q000276:immunology / Q000503:physiopathology; D019590:Nucleocapsid Proteins; D009698:Nucleoproteins; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology; D014162:Transfection; D014407:Tumor Cells, Cultured; D014758:Viral Core Proteins / Q000235:genetics / Q000276:immunology
D015703:Antigens, CD; D051940:B7-2 Antigen; C497964:CD86 protein, human; C497965:Cd86 protein, mouse; D008562:Membrane Glycoproteins; D019590:Nucleocapsid Proteins; D009698:Nucleoproteins; D014758:Viral Core Proteins