Variants of the fatty acid-binding protein 2 gene are not associated with coronary heart disease in nondiabetic subjects and in patients with NIDDM.
OBJECTIVE
To investigate the association of variants of the fatty acid-binding protein (FABP) 2 gene with coronary heart disease (CHD) in nondiabetic subjects and in patients with NIDDM.


METHODS
Cross-sectional study included 135 nondiabetic and 79 NIDDM subjects with stenosis (> 50%) in at least two coronary arteries. A group of 81 healthy nondiabetic men without CHD served as a control population. All exons and intron-exon junctions of the FABP2 gene were amplified with the polymerase chain reaction, and variants were screened with the single-strand conformation polymorphism analysis.


RESULTS
The allele frequency of an amino acid polymorphism (alanine-->threonine) in codon 54 of exon 2 of the FABP2 gene was 0.26 in nondiabetic subjects with CHD and 0.27 in NIDDM subjects with CHD. Other variants (GTA 118 GTC, GCGCA-->GCACA in the 3'-noncoding region, and the number of ATT repeats in intron 2) also did not associate with CHD.


CONCLUSIONS
The variants of the FABP2 gene are not likely to contribute to the risk of CHD in Finnish nondiabetic and NIDDM subjects.
Saarinen
L
L
Department of Medicine, University of Kuopio, Finland.
Pulkkinen
A
A
Kareinen
A
A
Heikkinen
S
S
Lehto
S
S
Laakso
M
M
D000483:Alleles; D019943:Amino Acid Substitution / Q000235:genetics; D001786:Blood Glucose / Q000378:metabolism; D001794:Blood Pressure / Q000502:physiology; D015992:Body Mass Index; D002352:Carrier Proteins / Q000235:genetics*; D003062:Codon / Q000235:genetics; D003327:Coronary Disease / Q000235:genetics*; D003430:Cross-Sectional Studies; D003627:Data Interpretation, Statistical; D003924:Diabetes Mellitus, Type 2 / Q000235:genetics*; D003971:Diastole; D005215:Fasting; D000071438:Fatty Acid-Binding Protein 7; D050556:Fatty Acid-Binding Proteins; D005227:Fatty Acids / Q000378:metabolism; D005260:Female; D005787:Gene Frequency; D014644:Genetic Variation; D006801:Humans; D007328:Insulin / Q000097:blood; D008297:Male; D008875:Middle Aged; D018996:Myelin P2 Protein / Q000235:genetics*; D009363:Neoplasm Proteins; D017354:Point Mutation / Q000235:genetics; D011110:Polymorphism, Genetic; D018807:Polymorphism, Single-Stranded Conformational; D013599:Systole; D018911:Trinucleotide Repeats / Q000235:genetics; D025521:Tumor Suppressor Proteins
D001786:Blood Glucose; D002352:Carrier Proteins; D003062:Codon; C493404:FABP2 protein, human; C107608:FABP7 protein, human; C493405:Fabp2 protein, mouse; D000071438:Fatty Acid-Binding Protein 7; D050556:Fatty Acid-Binding Proteins; D005227:Fatty Acids; D007328:Insulin; D018996:Myelin P2 Protein; D009363:Neoplasm Proteins; D025521:Tumor Suppressor Proteins