Endothelial nitric oxide synthase in vascular endothelium of rat hippocampus after ischemia: evidence and significance.
Electron microscopy immunocytochemical study was performed to clarify ultrastructural localization and role of endothelial nitric oxide synthase (EC-NOS) in the endothelial cells (EC) of rat hippocampal vessels after transient cerebral ischemia. EC-NOS immunoreactivity was found in the endothelial cells in association with plasma membrane, sub-plasmalemmal vesicles, basal membrane and in cytosol (cytoplasm free of subcellular organelles). A sharp transient increase in immunoreactivity of NOS was observed at 10 min up to 1 hour after ischemia. The results of the present study indicate that NO, as a potent vasodilator, may play a protective role in ischemic brain damage.
Gajkowska
B
B
Laboratory of the Ultrastructure of the Nervous System, Polish Academy of Sciences, Warszawa.
Mossakowski
M J
MJ
D000818:Animals; D002462:Cell Membrane / Q000648:ultrastructure; D003593:Cytoplasm / Q000648:ultrastructure; D003600:Cytosol / Q000648:ultrastructure; D004730:Endothelium, Vascular / Q000201:enzymology* / Q000648:ultrastructure; D006624:Hippocampus / Q000201:enzymology* / Q000648:ultrastructure; D007150:Immunohistochemistry; D002546:Ischemic Attack, Transient / Q000201:enzymology* / Q000473:pathology; D008297:Male; D019001:Nitric Oxide Synthase / Q000648:ultrastructure*; D015388:Organelles / Q000648:ultrastructure; D051381:Rats; D017208:Rats, Wistar
D019001:Nitric Oxide Synthase