title: Cloning and chromosomal localization of a new member of the bHLH/PAS transcription factor family.
abstract: Here we report the cloning and primary characterization of TIC, a new member of the bHLH/PAS domain family of transcription factors. Northern blot analysis indicates that the 3.3-kb Tic mRNA is widely expressed. A polyclonal antibody against TIC identifies multiple protein species in most cell lines and tissues tested, suggesting that alternative splicing may result in the production of protein isoforms. Interspecies backcross and FISH mapping have been used to localize the Tic gene to mouse Chromosome (Chr) 7 F2-F3, given the locus name Arntl. FISH mapping was also used to localize the human gene to Chr 11p15.
authors.affiliation: AMGEN Research Institute, Department of Medical Biophysics and Ontario Cancer Institute, University of Toronto, 620 University Ave, Suite 706, Toronto, Ontario, Canada, M5G 2C1.
authors.forename: C D
authors.initials: CD
authors.lastname: Wolting
authors.forename: C J
authors.initials: CJ
authors.lastname: McGlade
mesh_terms: D056930:ARNTL Transcription Factors; D017398:Alternative Splicing; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D051792:Basic Helix-Loop-Helix Proteins; D002874:Chromosome Mapping; D002880:Chromosomes, Human, Pair 11; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000737:chemistry; D018257:Helix-Loop-Helix Motifs / Q000235:genetics*; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D051379:Mice; D008969:Molecular Sequence Data; D015534:Trans-Activators / Q000235:genetics; D014157:Transcription Factors / Q000235:genetics*; D000099162:Endothelial PAS Domain-Containing Protein 1
chemical_list: D056930:ARNTL Transcription Factors; D051792:Basic Helix-Loop-Helix Proteins; D018076:DNA, Complementary; D015534:Trans-Activators; D014157:Transcription Factors; D000099162:Endothelial PAS Domain-Containing Protein 1; C538960:BMAL1 protein, human; C538961:Bmal1 protein, mouse