Expression of Ets-1 transcription factor in relation to angiogenesis in the healing process of gastric ulcer.
Ets-1 is a transcription factor known to control the expression of genes involved in extracellular matrix remodeling. The purpose of this study is to evaluate the expression of Ets-1 in the process of healing of ulceration in the rats. The time-dependent changes and distribution of Ets-1 in the margins of ulcer were examined. Ets-1 did not express in the normal gastric mucosa. In the marginal granulation tissue, fibroblasts and endothelial cells of capillaries were immunopositive for Ets-1. Ets-1 expression was significantly increased at the early phase, and returned to normal levels at the scarred phase. Serial sectioning revealed that fibroblasts and endothelial cells also expressed MMP-1. Protein levels and mRNA expression of Ets-1 were confirmed by Western blotting and RT-PCR. These findings suggest that Ets-1 plays an important role in angiogenesis in the early phase of ulcer healing.
Ito
M
M
Department of Molecular-Pathology, Nagasaki University School of Medicine, Japan. m-ito@net.nagasaki-u.ac.jp
Nakayama
T
T
Naito
S
S
Matsuu
M
M
Shichijo
K
K
Sekine
I
I
D000818:Animals; D001483:Base Sequence; D017931:DNA Primers / Q000235:genetics; D005753:Gastric Mucosa / Q000378:metabolism / Q000473:pathology; D015870:Gene Expression; D007150:Immunohistochemistry; D008297:Male; D018919:Neovascularization, Physiologic; D016133:Polymerase Chain Reaction; D050784:Proto-Oncogene Protein c-ets-1; D011518:Proto-Oncogene Proteins / Q000235:genetics* / Q000378:metabolism*; D050783:Proto-Oncogene Proteins c-ets; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D013276:Stomach Ulcer / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D014157:Transcription Factors / Q000235:genetics* / Q000378:metabolism*; D014945:Wound Healing / Q000235:genetics / Q000502:physiology*
D017931:DNA Primers; C494043:Ets1 protein, rat; D050784:Proto-Oncogene Protein c-ets-1; D011518:Proto-Oncogene Proteins; D050783:Proto-Oncogene Proteins c-ets; D012333:RNA, Messenger; D014157:Transcription Factors