title: Elevated levels of 8-iso-prostaglandin F2alpha in pericardial fluid of patients with heart failure: a potential role for in vivo oxidant stress in ventricular dilatation and progression to heart failure.
abstract: BACKGROUND
  It has been suggested that oxidant stress may play a role in the pathophysiology of heart failure. However, no definitive information is available because most previous approaches used to measure oxidant stress are nonspecific, inaccurate, and unreliable.
  
  
  RESULTS
  To evaluate oxidant stress in the heart, we measured pericardial fluid levels of 8-iso-prostaglandin F2alpha (8-iso-PGF2alpha), a specific and quantitative marker of oxidant stress in vivo, in a series of 51 consecutive patients with ischemic and/or valvular heart disease referred for cardiac surgery. Pericardial levels of 8-iso-PGF2alpha were correlated with the functional severity of heart failure (NYHA classification) and with echocardiographic indices of ventricular dilatation measured by independent physicians. Pericardial levels of 8-iso-PGF2alpha were significantly increased in patients with symptomatic heart failure compared with asymptomatic patients and gradually increased with the functional severity of heart failure (P=.0003). In addition, pericardial levels of 8-iso-PGF2alpha were significantly correlated with left ventricular end-diastolic and end-systolic diameters (P=.008 and .026, respectively).
  
  
  CONCLUSIONS
  Pericardial levels of 8-iso-PGF2alpha increase with the functional severity of heart failure and are associated with ventricular dilatation. These data suggest an important role for in vivo oxidant stress on ventricular remodeling and the progression to heart failure.
authors.affiliation: Institut National de la Santé et de la Recherche Médicale, IFR Circulation Lariboisière, INSERM U 141, Hôpital Bichat, Paris, France.
authors.forename: Z
authors.initials: Z
authors.lastname: Mallat
authors.forename: I
authors.initials: I
authors.lastname: Philip
authors.forename: M
authors.initials: M
authors.lastname: Lebret
authors.forename: D
authors.initials: D
authors.lastname: Chatel
authors.forename: J
authors.initials: J
authors.lastname: Maclouf
authors.forename: A
authors.initials: A
authors.lastname: Tedgui
mesh_terms: D000328:Adult; D000368:Aged; D015237:Dinoprost / Q000031:analogs & derivatives* / Q000378:metabolism*; D028441:F2-Isoprostanes; D005260:Female; D006333:Heart Failure / Q000378:metabolism* / Q000503:physiopathology*; D006352:Heart Ventricles / Q000503:physiopathology*; D006801:Humans; D008297:Male; D008875:Middle Aged; D009200:Myocardial Contraction / Q000502:physiology*; D018384:Oxidative Stress; D010496:Pericardium / Q000378:metabolism*
chemical_list: D028441:F2-Isoprostanes; C075750:8-epi-prostaglandin F2alpha; D015237:Dinoprost