title: Alpha-viniferin: a prostaglandin H2 synthase inhibitor from root of Carex humilis.
abstract: An inhibitor on cyclooxygenase activity of prostaglandin H2 synthase was purified from the root of Carex humilis Leyss (Cyperaceae) by a variety of column chromatographic methods. As a result of the structure analysis by FAB-mass, 1H-NMR, and 13C-NMR spectral data, the active compound was identified as (+)-alpha-viniferin, an oligomeric stilbene characterized originally from Caragana chamlagu Lamarck (Leguminosae). (+)-alpha-Viniferin exhibited a dose-dependent inhibition on cyclooxygenase activity, where 50% of inhibition (IC50) was shown at a final concentration of about 7 microM. Resveratrol, a putative building block of oligomeric stilbenes, also inhibited the cyclooxygenase activity. The inhibitory potency of (+)-alpha-viniferin was about 3- to 4-fold stronger than that of resveratrol on cyclooxygenase activity of prostaglandin H2 synthase partially purified from sheep seminal vesicles.
authors.affiliation: College of Pharmacy, Yeungnam University, Kyongsan, Korea.
authors.forename: S H
authors.initials: SH
authors.lastname: Lee
authors.forename: N H
authors.initials: NH
authors.lastname: Shin
authors.forename: S H
authors.initials: SH
authors.lastname: Kang
authors.forename: J S
authors.initials: JS
authors.lastname: Park
authors.forename: S R
authors.initials: SR
authors.lastname: Chung
authors.forename: K R
authors.initials: KR
authors.lastname: Min
authors.forename: Y
authors.initials: Y
authors.lastname: Kim
mesh_terms: D000818:Animals; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000494:pharmacology*; D001572:Benzofurans / Q000494:pharmacology*; D016861:Cyclooxygenase Inhibitors / Q000494:pharmacology*; D066298:In Vitro Techniques; D008297:Male; D008861:Microsomes / Q000187:drug effects / Q000201:enzymology; D010936:Plant Extracts / Q000494:pharmacology*; D018517:Plant Roots; D006109:Poaceae / Q000737:chemistry; D011451:Prostaglandin-Endoperoxide Synthases / Q000378:metabolism*; D012669:Seminal Vesicles / Q000187:drug effects / Q000201:enzymology; D012756:Sheep
chemical_list: D000894:Anti-Inflammatory Agents, Non-Steroidal; D001572:Benzofurans; D016861:Cyclooxygenase Inhibitors; D010936:Plant Extracts; C064176:alpha-viniferin; D011451:Prostaglandin-Endoperoxide Synthases