Somatic cell cloned transgenic bovine neurons for transplantation in parkinsonian rats.
Parkinson's disease symptoms can be improved by transplanting fetal dopamine cells into the putamen of parkinsonian patients. Because the supply of human donor tissue is limited and variable, an alternative and genetically modifiable non-human source of tissue would be valuable. We have generated cloned transgenic bovine embryos, 42% of which developed beyond 40 days. Dopamine cells collected from the ventral mesencephalon of the cloned fetuses 42 to 50 days post-conception survived transplantation into immunosuppressed parkinsonian rats and cells from cloned and wild-type embryos improved motor performance. Somatic cell cloning can efficiently produce transgenic animal tissue for treating parkinsonism.
Zawada
W M
WM
Department of Medicine, University of Colorado School of Medicine, Denver 80262, USA.
Cibelli
J B
JB
Choi
P K
PK
Clarkson
E D
ED
Golueke
P J
PJ
Witta
S E
SE
Bell
K P
KP
Kane
J
J
Ponce de Leon
F A
FA
Jerry
D J
DJ
Robl
J M
JM
Freed
C R
CR
Stice
S L
SL
D000818:Animals; D030801:Animals, Genetically Modified; D002417:Cattle; D019976:Cloning, Organism; D004298:Dopamine / Q000096:biosynthesis*; D004628:Embryonic Structures / Q000637:transplantation; D007763:Lac Operon; D008636:Mesencephalon / Q000196:embryology / Q000637:transplantation; D009474:Neurons / Q000637:transplantation*; D010300:Parkinson Disease / Q000628:therapy*; D051381:Rats; D014183:Transplantation, Heterologous / Q000379:methods*
D004298:Dopamine