Identification of a human cDNA clone that mediates adherence of pathogenic Neisseria to non-binding cells.
Neisseria gonorrhoeae and Neisseria meningitidis are exclusively human pathogens. A crucial property of the pathogenicity of neisserial infection is the ability to adhere to human epithelial cells. Pili mediate adherence of these bacteria to target cells and thereby promote colonization and infection of mucosal surfaces. In order to identify and to learn more about the initial event during infection, a cDNA clone from a human cervical epithelial cell line was identified in a panning experiment using purified gonococcal pili as probe. Upon transfection of the cloned cDNA into COS-7 cells, both gonococci and meningococci adhered to these otherwise non-binding cells. The deduced amino acid sequence of the cDNA clone showed homology to a recently reported human cDNA, called WWP2, that encodes an N-terminal C2-like domain. The C2 domain has been shown to bind membrane phospholipids in a calcium-dependent manner and is thought to function in the intracellular compartmentalization of proteins. Antiserum raised against the product encoded by the cDNA did not inhibit bacterial adherence, indicating that the cloned gene is most likely involved in up-regulation of a surface receptor for pathogenic Neisseria.
Jonsson
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AB
Microbiology and Tumorbiology Center, Karolinska Institutet, Stockholm, Sweden. Ann-Beth.Jonsson@mtc.ki.se
D000818:Animals; D001422:Bacterial Adhesion / Q000502:physiology*; D019556:COS Cells; D002352:Carrier Proteins / Q000502:physiology*; D002460:Cell Line; D002584:Cervix Uteri / Q000166:cytology; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics*; D004847:Epithelial Cells / Q000382:microbiology; D005260:Female; D010861:Fimbriae, Bacterial; D006801:Humans; D008969:Molecular Sequence Data; D009344:Neisseria gonorrhoeae / Q000472:pathogenicity*; D009345:Neisseria meningitidis / Q000472:pathogenicity*; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid
D002352:Carrier Proteins; D018076:DNA, Complementary; C112397:Neisseria adherence-associated protein, human