title: Neurons in the hypothalamic paraventricular nucleus that project to the rostral ventrolateral medulla are activated by haemorrhage.
abstract: The retrogradely-transported tracer, rhodamine-tagged microspheres, was injected into the pressor region of the rostral ventrolateral medulla (RVLM) to identify paraventricular neurons in the hypothalamus that project to the RVLM. The protein, Fos, was detected immunohistochemically and used to highlight neurons that were activated by a hypotensive haemorrhage. Compared to controls, Fos production was increased by approximately 3-fold in the paraventricular nucleus (P<0.009) and there was a significant increase in the number of retrogradely-labelled cells that expressed Fos. These represented 5% of the retrogradely-labelled cell population. The results suggest that a small subpopulation of PVN neurons projecting to the RVLM are activated by haemorrhage and may be involved in the reflex responses initiated by that stimulus.
authors.affiliation: Department of Medicine, Monash University, Monash Medical Centre, Clayton Rd., Clayton 3168, Melbourne, Victoria, Australia. emilio@badoer@med.monash.edu.au
authors.forename: E
authors.initials: E
authors.lastname: Badoer
authors.forename: J
authors.initials: J
authors.lastname: Merolli
mesh_terms: D000818:Animals; D001931:Brain Mapping; D002543:Cerebral Hemorrhage / Q000378:metabolism / Q000473:pathology / Q000503:physiopathology*; D007124:Immunoenzyme Techniques; D008526:Medulla Oblongata / Q000737:chemistry / Q000502:physiology*; D008863:Microspheres; D009419:Nerve Tissue Proteins / Q000032:analysis; D009434:Neural Pathways / Q000737:chemistry / Q000502:physiology; D009474:Neurons / Q000502:physiology*; D010286:Paraventricular Hypothalamic Nucleus / Q000737:chemistry / Q000166:cytology / Q000502:physiology*; D016760:Proto-Oncogene Proteins c-fos / Q000032:analysis; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D009419:Nerve Tissue Proteins; D016760:Proto-Oncogene Proteins c-fos