BMPR-II expression and OP-1 effects in developing chicken retinal explants.
Low-stringency PCR was used to isolate the chicken homologue of the human bone morphogenetic protein receptor type II (BMPR-II). In situ hybridization localized mRNA expression for this serine/threonine kinase receptor. It was weakly expressed in the vitreal side of the neural retina at E6. In the E7 chicken, fairly strong labelling was found in cells of the internal part of the neural retina. At E9, strong labelling was found in the region of the retinal ganglion cells. Explants of E6 retina exposed to osteogenic protein-1 (OP-1/BMP-7), exhibited dense retinal fibre outgrowth. This suggests that BMPR-II may form a signalling receptor complex important for retinal development. OP-1 and related ligands may serve functions supplementary to those of neurotrophins.
Carri
N G
NG
Department of Neuroscience, Uppsala University, Sweden.
Bengtsson
H
H
Charette
M F
MF
Ebendal
T
T
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D055419:Bone Morphogenetic Protein 7; D052006:Bone Morphogenetic Protein Receptors, Type II; D019485:Bone Morphogenetic Proteins / Q000494:pharmacology*; D002454:Cell Differentiation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D002642:Chick Embryo; D003001:Cloning, Molecular; D008969:Molecular Sequence Data; D017346:Protein Serine-Threonine Kinases / Q000235:genetics*; D011956:Receptors, Cell Surface / Q000235:genetics*; D012160:Retina / Q000187:drug effects* / Q000196:embryology; D012165:Retinal Ganglion Cells / Q000187:drug effects; D016212:Transforming Growth Factor beta
C525819:BMP7 protein, human; D055419:Bone Morphogenetic Protein 7; D019485:Bone Morphogenetic Proteins; D011956:Receptors, Cell Surface; D016212:Transforming Growth Factor beta; D017346:Protein Serine-Threonine Kinases; D052006:Bone Morphogenetic Protein Receptors, Type II