title: Chiaroscuro hematopoietic stem cell.
abstract: These observations suggest several immediate clinical strategies. In gene therapy, approaches could be targeted to obtain cycling of hematopoietic stem cells and gene-carrying retrovirus vector integration followed by engraftment at an appropriate time interval which favors engraftment. The same type of approach can be utilized for stem cell expansion approaches. Alternatively marrow or peripheral stem cell engraftment can be obtained with minimal to no toxicity in allochimeric strategies in such diseases as sickle cell anemia or thalassemia. A similar approach could be useful in obtaining cell engraftment with minimal toxicity in therapies employing cellular immune (T-cell and NK-cell) attack against cancer. These areas of clinical application are outline in Table 3.
authors.affiliation: Cancer Center, University of Massachusetts Medical Center, Worcester 01605, USA.
authors.forename: P
authors.initials: P
authors.lastname: Quesenberry
authors.forename: M
authors.initials: M
authors.lastname: Habibian
authors.forename: M
authors.initials: M
authors.lastname: Dooner
authors.forename: S
authors.initials: S
authors.lastname: Zhong
authors.forename: J
authors.initials: J
authors.lastname: Reilly
authors.forename: S
authors.initials: S
authors.lastname: Peters
authors.forename: P
authors.initials: P
authors.lastname: Becker
authors.forename: C
authors.initials: C
authors.lastname: Grimaldi
authors.forename: J
authors.initials: J
authors.lastname: Carlson
authors.forename: P
authors.initials: P
authors.lastname: Reddy
authors.forename: S
authors.initials: S
authors.lastname: Nilsson
authors.forename: F M
authors.initials: FM
authors.lastname: Stewart
mesh_terms: D000818:Animals; D002454:Cell Differentiation; D016207:Cytokines / Q000494:pharmacology; D005260:Female; D015316:Genetic Therapy; D006410:Hematopoiesis; D018380:Hematopoietic Stem Cell Transplantation; D006412:Hematopoietic Stem Cells / Q000166:cytology* / Q000187:drug effects; D006801:Humans; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008954:Models, Biological; D010641:Phenotype
chemical_list: D016207:Cytokines