PIG-B: a homemade monophasic cocktail for the extraction of RNA.
An inexpensive monophasic reagent has been developed for the extraction of total RNA from cells or tissues. The main ingredients of the reagent are Phenol, Isoamyl alcohol, Guanidinium isothiocyanate, and Beta-mercaptoethanol (PIG-B). The quality and yield of RNA obtained by this reagent is at par with that obtained by TRIzol, an expensive but widely used monophasic reagent available commercially. The complete composition and method of preparation of PIG-B is provided to aid preparation of the reagent in the laboratory.
Weber
K
K
Department of Orthopedics, Mayo Clinic, Rochester, MN.
Bolander
M E
ME
Sarkar
G
G
D006146:Guanidines; D007202:Indicators and Reagents; D017879:Isothiocyanates; D008623:Mercaptoethanol; D000439:Pentanols; D010636:Phenols; D012313:RNA / Q000302:isolation & purification*
D006146:Guanidines; D007202:Indicators and Reagents; D017879:Isothiocyanates; D000439:Pentanols; D010636:Phenols; C054435:guanidine isothiocyanate; D008623:Mercaptoethanol; D012313:RNA; C029683:isopentyl alcohol