Adrenomedullin production is correlated with differentiation in human leukemia cell lines and peripheral blood monocytes.
We demonstrated that adrenomedullin (AM) is produced and secreted from human leukemia cell lines (THP-1 and HL-60) as well as peripheral blood granulocytes, lymphocytes, monocytes and monocyte-derived macrophages. Immunoreactive AM accumulated in the culture media of THP-1 and HL-60 cells increased according to their differentiation into macrophage-like cells. Retinoic acid exerted synergistic effects on AM secretion from THP-1 and HL-60 cells when administered with tumor necrosis factor-alpha, lipopolysaccharide or 12-O-tetradecanoyl phorbol-13-acetate. AM was shown to increase the scavenger receptor activity on THP-1 cells. Thus, monocytes/macrophages should be recognized as sources of AM, and the secreted AM may modulate the function of macrophages.
Kubo
A
A
National Cardiovascular Center Research Institute, Suita, Osaka, Japan.
Minamino
N
N
Isumi
Y
Y
Kangawa
K
K
Dohi
K
K
Matsuo
H
H
D053607:Adrenomedullin; D002454:Cell Differentiation; D006098:Granulocytes / Q000378:metabolism; D018922:HL-60 Cells / Q000166:cytology / Q000378:metabolism; D006801:Humans; D008264:Macrophages / Q000166:cytology / Q000378:metabolism*; D008565:Membrane Proteins; D009000:Monocytes / Q000166:cytology / Q000378:metabolism*; D010455:Peptides / Q000378:metabolism*; D011971:Receptors, Immunologic / Q000378:metabolism; D018110:Receptors, Lipoprotein; D051116:Receptors, Scavenger; D051122:Scavenger Receptors, Class B; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology; D014212:Tretinoin / Q000494:pharmacology; D014407:Tumor Cells, Cultured / Q000166:cytology* / Q000378:metabolism
D008565:Membrane Proteins; D010455:Peptides; D011971:Receptors, Immunologic; D018110:Receptors, Lipoprotein; D051116:Receptors, Scavenger; C454739:Scarb1 protein, mouse; D051122:Scavenger Receptors, Class B; D053607:Adrenomedullin; D014212:Tretinoin; D013755:Tetradecanoylphorbol Acetate