title: Constitutive choline transport in Pseudomonas aeruginosa.
abstract: Pseudomonas aeruginosa has a choline uptake system which is expressed in bacteria grown in the presence of succinate and ammonium chloride as the carbon and nitrogen source, respectively. This system obeys Michaelis-Menten kinetics with an apparent Km value of 53 microM; its activity is not inhibited by high osmolarities in the medium but is partially inhibited by choline metabolites such as betaine and dimethylglycine.
authors.affiliation: Departamento de Biología Molecular, Universidad Nacional de Río Cuarto, Córdoba, Argentina.
authors.forename: G I
authors.initials: GI
authors.lastname: Lucchesi
authors.forename: C
authors.initials: C
authors.lastname: Pallotti
authors.forename: A T
authors.initials: AT
authors.lastname: Lisa
authors.forename: C E
authors.initials: CE
authors.lastname: Domenech
mesh_terms: D000643:Ammonium Chloride / Q000494:pharmacology; D001622:Betaine / Q000494:pharmacology; D001692:Biological Transport; D002794:Choline / Q000378:metabolism* / Q000494:pharmacology; D007700:Kinetics; D009994:Osmolar Concentration; D011550:Pseudomonas aeruginosa / Q000378:metabolism*; D012521:Sarcosine / Q000031:analogs & derivatives / Q000494:pharmacology; D012965:Sodium Chloride / Q000494:pharmacology; D013386:Succinates / Q000494:pharmacology
chemical_list: D013386:Succinates; D000643:Ammonium Chloride; D001622:Betaine; D012965:Sodium Chloride; C025138:dimethylglycine; D002794:Choline; D012521:Sarcosine