[An experimental study on the relationship between BMP's activity and its carrier].
To find out the relationship between human bone morphogenetic protein's activity and its carrier and to document the clinical application of BMP, we investigated six kinds of BMP carrier's influence upon hBMP's osteogenic activity with small sample size at first. Then three kinds of carriers selected from the six, added with the seventh carrier, were investigated with large sample size. The result showed that the compound of hBMP and hDDBM had best osteogenic ability. The human bone matrix had been formerly demineralized and extracted without hBMP. The hDDBM showed porous structure under SEM, its mean pore's diameter was 127 +/- 34 microns. It was demonstrated that the function of demineralized bone matrix extracted without BMP (i.e. DDBM) was related not only to its pore's diameter, but to the fibrolike matter in it as well. With the method of BMP bioassay, BMP was composited with carrier, then the compound was injected into the calf of mouse. This made BMP's bioassay become simple and reliable.
Yu
J
J
Institute of Sports Medicine, Beijing Medical University.
Qu
M
M
Tian
D
D
D000818:Animals; D018775:Bone Demineralization Technique; D001857:Bone Matrix / Q000637:transplantation* / Q000648:ultrastructure; D019485:Bone Morphogenetic Proteins / Q000494:pharmacology*; D051379:Mice; D008855:Microscopy, Electron, Scanning; D010012:Osteogenesis / Q000187:drug effects*; D011897:Random Allocation
D019485:Bone Morphogenetic Proteins