title: Dose-dependent selective cytotoxicity of extracts from marine green alga, Cladophoropsis vaucheriaeformis, against mouse leukemia L1210 cells.
abstract: The selective cytotoxic activity of extracts from two marine green algae, Cladophoropsis vaucheriaeformis and Halimeda discoidea, was examined via a dose response assay against mouse leukemia L1210 cells and normal NIH-3T3 cells. The MeOH-extract from C. vaucheriaeformis showed selective cytotoxicity to L1210 cells at concentrations ranging from 50 to 100 microg/ml. In particular, the greatest selectivity for cytotoxic activity was found at the concentration of 50 microg/ml, at which the growth of L1210 cells was inhibited completely and that of NIH-3T3 was not affected at all. However, MeOH extracts from the red alga Laurencia okamurae and the brown alga Dictyopteris undulata, which displayed non-selective cytotoxicity in our previous screening program, did not show similar selective cytotoxicity at any concentrations tested. These results indicate that the marine green alga C. vaucheriaeformis may contain a unique antitumor substance with selective cytotoxic activity against L1210 cells. Our results also suggest that this active substance might be of low molecular weight and therefore MeOH-soluble.
authors.affiliation: Marine and Highland Bioscience Center, Saga University, Karatsu, Japan.
authors.forename: H
authors.initials: H
authors.lastname: Harada
authors.forename: Y
authors.initials: Y
authors.lastname: Kamei
mesh_terms: D000818:Animals; D000970:Antineoplastic Agents / Q000737:chemistry* / Q000494:pharmacology*; D000460:Chlorophyta / Q000737:chemistry*; D002850:Chromatography, Gel; D006358:Hot Temperature; D007939:Leukemia L1210 / Q000188:drug therapy*; D051379:Mice; D008970:Molecular Weight; D012995:Solubility; D014407:Tumor Cells, Cultured
chemical_list: D000970:Antineoplastic Agents