title: Magnetic resonance imaging detects suppression of tumor vascular permeability after administration of antibody to vascular endothelial growth factor.
abstract: Macromolecular contrast medium-enhanced magnetic resonance imaging (MRI) and tumor-volume measurements were applied to monitor the effects of anti-vascular endothelial growth factor (anti-VEGF) antibody on microvascular characteristics and tumor growth of MDA-MB-435 human breast cancer cells implanted in nude rats. Administration of anti-VEGF antibody (three 1 mg doses at 3-day intervals) induced significant reductions in tumor growth rates (p < 0.05) and in MRI-assayed microvascular permeabilities (p < 0.05). Results of the study were consistent with previous observations that new microvessels formed in response to angiogenesis are hyperpermeable, and with the hypothesis that hyperpermeability is a mechanistic element in angiogenesis. Variations in tumor-vessel hyperpermeability can be measured by contrast-enhanced MRI, which may prove useful for assessing antiangiogenesis therapy.
authors.affiliation: Department of Radiology, University of California, San Francisco, USA.
authors.forename: C D
authors.initials: CD
authors.lastname: Pham
authors.forename: T P
authors.initials: TP
authors.lastname: Roberts
authors.forename: N
authors.initials: N
authors.lastname: van Bruggen
authors.forename: O
authors.initials: O
authors.lastname: Melnyk
authors.forename: J
authors.initials: J
authors.lastname: Mann
authors.forename: N
authors.initials: N
authors.lastname: Ferrara
authors.forename: R L
authors.initials: RL
authors.lastname: Cohen
authors.forename: R C
authors.initials: RC
authors.lastname: Brasch
mesh_terms: D000230:Adenocarcinoma / Q000098:blood supply* / Q000503:physiopathology; D000818:Animals; D000906:Antibodies / Q000494:pharmacology*; D001810:Blood Volume / Q000187:drug effects; D001943:Breast Neoplasms / Q000098:blood supply* / Q000503:physiopathology; D002199:Capillary Permeability / Q000187:drug effects*; D002455:Cell Division / Q000187:drug effects; D016228:Endothelial Growth Factors / Q000276:immunology*; D005260:Female; D006801:Humans; D007091:Image Processing, Computer-Assisted; D008222:Lymphokines / Q000276:immunology*; D008279:Magnetic Resonance Imaging / Q000379:methods; D051379:Mice; D009368:Neoplasm Transplantation; D051381:Rats; D011923:Rats, Nude; D014183:Transplantation, Heterologous; D014407:Tumor Cells, Cultured; D042461:Vascular Endothelial Growth Factor A; D042442:Vascular Endothelial Growth Factors
chemical_list: D000906:Antibodies; D016228:Endothelial Growth Factors; D008222:Lymphokines; D042461:Vascular Endothelial Growth Factor A; D042442:Vascular Endothelial Growth Factors