title: The cbaAB genes for bo3-type cytochrome c oxidase in Bacillus stearothermophilus.
abstract: Structural genes were cloned for cytochrome bo3-type cytochrome c oxidase recently isolated from a Gram-positive thermophile Bacillus stearothermophilus. Sequencing and Northern blotting analyses indicated that the two genes cbaA and cbaB composed an operon encoding for subunits I and II, respectively, and that the oxidase was SoxB-type. They are the first genes for a SoxB-type cytochrome c oxidase whose natural substrate is known.
authors.affiliation: Department of Biochemical Engineering and Science, Kyushu Institute of Technology, 680-4 Kawazu, Iizuka, Fukuoka 820-8502, Japan.
authors.forename: K
authors.initials: K
authors.lastname: Nikaido
authors.forename: S
authors.initials: S
authors.lastname: Noguchi
authors.forename: J
authors.initials: J
authors.lastname: Sakamoto
authors.forename: N
authors.initials: N
authors.lastname: Sone
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D002417:Cattle; D002874:Chromosome Mapping; D003001:Cloning, Molecular; D003573:Cytochrome b Group; D003574:Cytochrome c Group / Q000235:genetics; D003580:Cytochromes; D017931:DNA Primers / Q000235:genetics; D003576:Electron Transport Complex IV / Q000737:chemistry / Q000235:genetics*; D029968:Escherichia coli Proteins; D005798:Genes, Bacterial; D001411:Geobacillus stearothermophilus / Q000201:enzymology* / Q000235:genetics*; D008969:Molecular Sequence Data; D010088:Oxidoreductases / Q000235:genetics; D016133:Polymerase Chain Reaction; D011487:Protein Conformation; D017386:Sequence Homology, Amino Acid
chemical_list: D003573:Cytochrome b Group; D003574:Cytochrome c Group; D003580:Cytochromes; D017931:DNA Primers; D029968:Escherichia coli Proteins; C083850:cytochrome bo3, E coli; D010088:Oxidoreductases; C064500:flavocytochrome c sulfide dehydrogenase; D003576:Electron Transport Complex IV