title: Effect of heparin on hemagglutination by equine arteritis virus.
abstract: Heparin inhibited hemagglutination (HA) by equine arteritis virus (EAV) as well as did HA by Aujeszky's disease virus (ADV), but failed to inhibit HA by parainfluenza virus type 3 (PIV-3). The minimal concentration of heparin required to inhibit 8 HA U of EAV was 0.1 U/ml. In addition, most EAV hemagglutinin was retained by heparin acrylic beads, as was ADV hemagglutinin, but was not PIV-3 hemagglutinin. Mouse erythrocytes failed to combine with the HA inhibitory factor of heparin. However, mouse erythrocytes treated with heparinase had greatly reduced agglutinability by EAV. All these findings suggest that a heparin-like molecule on the surface of mouse erythrocytes serves as the virus-cell receptor.
authors.affiliation: Department of Veterinary Epizootiology, College of Bioresource Sciences, Nihon University, Kanagawa, Japan.
authors.forename: Y
authors.initials: Y
authors.lastname: Sano
authors.forename: Y
authors.initials: Y
authors.lastname: Inaba
authors.forename: K
authors.initials: K
authors.lastname: Uwatoko
authors.forename: T
authors.initials: T
authors.lastname: Kubota
authors.forename: T
authors.initials: T
authors.lastname: Asagoe
authors.forename: J
authors.initials: J
authors.lastname: Kanaya
authors.forename: I J
authors.initials: IJ
authors.lastname: Pan
authors.forename: H
authors.initials: H
authors.lastname: Akashi
authors.forename: Y
authors.initials: Y
authors.lastname: Fukunaga
mesh_terms: D000818:Animals; D002417:Cattle; D002460:Cell Line; D007240:Equartevirus / Q000187:drug effects / Q000502:physiology*; D004912:Erythrocytes / Q000187:drug effects / Q000502:physiology / Q000821:virology; D006384:Hemagglutination / Q000187:drug effects*; D006385:Hemagglutination Inhibition Tests; D006386:Hemagglutination Tests; D006389:Hemagglutinins, Viral / Q000502:physiology; D006493:Heparin / Q000494:pharmacology*; D019763:Heparin Lyase / Q000494:pharmacology; D011558:Herpesvirus 1, Suid / Q000502:physiology; D007668:Kidney; D051379:Mice; D011817:Rabbits; D013552:Swine
chemical_list: D006389:Hemagglutinins, Viral; D006493:Heparin; D019763:Heparin Lyase