Determination of naproxen in liver and kidney tissues by electrokinetic capillary chromatography with laser-induced fluorescence detection.
Pharmacotherapy through the targeting of drugs is a promising new approach that requires adequate analytical methods capable of monitoring the free drug, the drug carrier and metabolites in body fluids and organs. A micellar electrokinetic capillary chromatography (MECC) based assay for analysis of naproxen (NAP) in extracts of hydrolyzed liver and kidney tissue homogenates using salicylate as internal standard and solute detection by laser-induced fluorescence is reported. The assay described uses 100 microliters of hydrolyzed tissue homogenate and has a detection limit of 0.07 microgram/ml. It is shown to be selective, reproducible (at a NAP level of 0.25 microgram/ml, intra-day and inter-day R.S.D. values are 3.73% and 6.39%, respectively), simple and economical (operates with inexpensive separation columns and small amounts of chemicals). It has been successfully applied to the assessment of the total NAP content within liver and kidney tissues of male Sprague Dawley rats that have been treated with NAP conjugated to human serum albumin (the drug targeting carrier) and free NAP. Compared to previously applied techniques, including high-performance liquid chromatography, MECC offers the advantage of having lower running costs and lower consumption of organic solvents.
Albrecht
C
C
Department of Clinical Pharmacology, University of Berne, Switzerland.
Thormann
W
W
D000818:Animals; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000008:administration & dosage / Q000032:analysis*; D004350:Drug Residues / Q000032:analysis*; D019075:Electrophoresis, Capillary / Q000379:methods*; D007275:Injections, Intravenous; D007668:Kidney / Q000737:chemistry*; D008099:Liver / Q000737:chemistry*; D008297:Male; D008823:Micelles; D009288:Naproxen / Q000008:administration & dosage / Q000032:analysis*; D051381:Rats; D017207:Rats, Sprague-Dawley
D000894:Anti-Inflammatory Agents, Non-Steroidal; D008823:Micelles; D009288:Naproxen