Modified Hirt procedure for rapid purification of extrachromosomal DNA from mammalian cells.
Arad
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Department of Hematology, Hebrew University-Hadassah Medical School, Jerusalem, Israel.
D000818:Animals; D019556:COS Cells; D002458:Cell Fractionation / Q000379:methods*; D011232:Chemical Precipitation; D002522:Chlorocebus aethiops; D002725:Chloroform; D004247:DNA / Q000302:isolation & purification*; D004279:DNA, Viral / Q000302:isolation & purification; D000431:Ethanol; D005057:Eukaryotic Cells / Q000737:chemistry*; D006863:Hydrogen-Ion Concentration; D019800:Phenol; D010957:Plasmids / Q000737:chemistry; D012259:Ribonuclease, Pancreatic; D012967:Sodium Dodecyl Sulfate; D014162:Transfection
D004279:DNA, Viral; D019800:Phenol; D012967:Sodium Dodecyl Sulfate; D000431:Ethanol; D002725:Chloroform; D004247:DNA; D012259:Ribonuclease, Pancreatic