title: Characterization of mixed micellar pseudostationary phases in electrokinetic chromatography using linear solvation energy relationships.
abstract: The influence of mixed micellar systems on retention and selectivity in micellar electrokinetic chromatography is examined using linear solvation energy relationships (LSER). Systems that were investigated include mixed bile salts [sodium deoxycholate (SDC) and sodium cholate (SC)] and mixed sodium dodecyl sulfate (SDS)-bile salt systems (e.g., SDS-SC and SDS-SDC). The retention behavior in individual and mixed micellar systems is primarily determined by size and hydrogen bond acceptor strengths of solutes. Through a comparative study of the LSER coefficients in the individual and mixed micellar systems, it was concluded that hydrogen bonding interactions have a significant effect on selectivity of these pseudostationary phases in electrokinetic chromatography. The interactive properties of the mixed micelles are different from the constituent individual micelles, however, the overall characteristics are closer to one of the bile salt micelles in the mixture even at the equimolar compositions.
authors.affiliation: North Carolina State University, Department of Chemistry, Raleigh 27695-8204, USA.
authors.forename: M G
authors.initials: MG
authors.lastname: Khaledi
authors.forename: J G
authors.initials: JG
authors.lastname: Bumgarner
authors.forename: M
authors.initials: M
authors.lastname: Hadjmohammadi
mesh_terms: D002756:Cholagogues and Choleretics / Q000737:chemistry*; D019826:Cholic Acid; D002793:Cholic Acids / Q000737:chemistry*; D003840:Deoxycholic Acid / Q000737:chemistry*; D019075:Electrophoresis, Capillary / Q000379:methods*; D006860:Hydrogen Bonding; D016014:Linear Models; D008823:Micelles; D009994:Osmolar Concentration; D012967:Sodium Dodecyl Sulfate / Q000737:chemistry*; D012997:Solvents / Q000737:chemistry; D013501:Surface-Active Agents / Q000737:chemistry*
chemical_list: D002756:Cholagogues and Choleretics; D002793:Cholic Acids; D008823:Micelles; D012997:Solvents; D013501:Surface-Active Agents; D003840:Deoxycholic Acid; D012967:Sodium Dodecyl Sulfate; D019826:Cholic Acid