title: Human papillomavirus type 33 DNA and E6-E7 transcripts in late passages of the UT-DEC-1 vaginal keratinocyte cell line.
abstract: Transcription of human papillomavirus (HPV) type 33 early region was analysed in the UT-DEC-1 keratinocyte cell line, which has been derived from a HPV-33-containing mild vaginal dysplasia. Fifteen cDNA clones from transcripts from the E6-E7 open reading frames were constructed and analysed. Most clones represented viral transcripts spliced within the E6 open reading frame, probably encoding the E7 protein. Interestingly, a less abundant unspliced transcript species with coding capacity for the full length E6 protein was found, reported here for the first time for the malignancy-associated HPV type 33.
authors.affiliation: Deutsches Krebsforschungszentrum, Applied Tumor Virology, Heidelberg, Germany.
authors.forename: E
authors.initials: E
authors.lastname: Auvinen
authors.forename: S
authors.initials: S
authors.lastname: Hietanen
authors.forename: L
authors.initials: L
authors.lastname: Gissmann
authors.forename: A
authors.initials: A
authors.lastname: Alonso
mesh_terms: D001483:Base Sequence; D002460:Cell Line; D017931:DNA Primers; D004279:DNA, Viral / Q000032:analysis*; D005260:Female; D006801:Humans; D015603:Keratinocytes / Q000821:virology; D009856:Oncogene Proteins, Viral / Q000096:biosynthesis*; D016366:Open Reading Frames; D027383:Papillomaviridae / Q000235:genetics / Q000302:isolation & purification*; D016133:Polymerase Chain Reaction; D014158:Transcription, Genetic; D014621:Vagina / Q000821:virology
chemical_list: D017931:DNA Primers; D004279:DNA, Viral; D009856:Oncogene Proteins, Viral