The yeast two-hybrid screening technique and its use in the study of protein-protein interactions in apoptosis.
The yeast two-hybrid technique provides a general approach for cloning cDNAs merely by exploiting the ability of their encoded proteins to bind to a protein of interest. The technique therefore offered a useful access to the analysis of the mechanisms of cell death at the initial stage of their study, when only a few of the proteins involved and very little about their mode of action were known. Conversely, the knowledge of cell death mechanisms gained by this technique provided a useful insight into both the potential and the limitations of this technique.
Wallach
D
D
Department of Biological Chemistry, Weizmann Institute, Israel. ivwalach@weizmann.weizmann.ac.il
Boldin
M P
MP
Kovalenko
A V
AV
Malinin
N L
NL
Mett
I L
IL
Camonis
J H
JH
D017209:Apoptosis / Q000235:genetics / Q000502:physiology*; D005656:Fungal Proteins / Q000235:genetics*; D006801:Humans; D009693:Nucleic Acid Hybridization / Q000379:methods*; D015398:Signal Transduction / Q000235:genetics; D015003:Yeasts / Q000235:genetics
D005656:Fungal Proteins