[Immunolabelling of collagen types I, III and IV, laminin and fibronectin in the human lens capsule].
OBJECTIVE
To localize collagen types I, III, and IV, laminin and fibronectin in the anterior human lens capsule.


METHODS
Twenty-one anterior capsules were sampled by capsulorhexis during extracapsular cataract extraction (mean age 71.5). All capsules were labelled by an immunostaining specific for each antibodies. Immunostaining of four capsules was revealed with immunoperoxydase and seventeen using indirect immunofluorescence.


RESULTS
Labelling of collagen types I and III was observed throughout the entire thickness of the capsule for each technique, the strongest labelling was found in the base of the epithelial cells with immunofluorescence. Collagen type IV was observed at the base of the epithelial cells whichever technique was used. Laminin could be detected in the inner layer of the capsule, using immunoperoxydase or immunofluorescence. No specific labelling was found for fibronectin using the two techniques.


CONCLUSIONS
Different kinds of collagens have been found in capsules, more particularly the type III. The latter does not appear on other ocular basement membrane. Because of this uneven distribution in the capsule's thickness, each collagen might have a specific function.
Majo
F
F
Service d'Ophtalmologie, CHU Jean Minjoz, Besançon.
Montard
M
M
Delbosc
B
B
Kantelip
B
B
D000368:Aged; D000369:Aged, 80 and over; D003094:Collagen / Q000032:analysis*; D005260:Female; D005353:Fibronectins / Q000032:analysis*; D019084:Fluorescent Antibody Technique, Indirect; D006801:Humans; D007124:Immunoenzyme Techniques; D007797:Laminin / Q000032:analysis*; D007903:Lens Capsule, Crystalline / Q000737:chemistry*; D008297:Male; D008875:Middle Aged
D005353:Fibronectins; D007797:Laminin; D003094:Collagen
Immunomarquage des collagènes I, III et IV, de la laminine et de la fibronectine dans la capsule cristallinienne humaine.