Evaluation of MAO activities on murine neuroblastoma cells upon acute or chronic treatment with aluminium (III) or tacrine.
Monoamine-oxidase catalyses the oxidative deamination of various primary amines such as norepinephrine, serotonin, dopamine and others. Such an enzyme exists under two well known isotypes, A and B. In the brain monoamine-oxidase activity increases with ageing. The present paper reports data regarding the evaluation on the activity of monoamine-oxidase A and B in murine neuroblastoma cells carried out by a radioassay upon acute or chronic treatment of cells by aluminium or tacrine. Our data show that aluminium has a pronounced activatory effect on both enzymatic isoforms either in acute or long-term adaptation treatment. Conversely, tacrine produces an inhibitory effect of both monoamine oxidase isotypes after an acute treatment, while an inhibitory effect on the isoform A and an activation of the isoform B in long-term adaptation was observed. Such effects are discussed in terms of aluminium neurotoxicity and therapeutic implications for tacrine.
Zatta
P
P
CNR-Center on Metalloproteins, Padova, Italy.
Nicosia
V
V
Zambenedetti
P
P
D000535:Aluminum / Q000633:toxicity*; D000818:Animals; D002250:Carbon Radioisotopes; D004789:Enzyme Activation / Q000187:drug effects; D007930:Leucine / Q000378:metabolism; D051379:Mice; D008995:Monoamine Oxidase / Q000187:drug effects / Q000378:metabolism*; D009447:Neuroblastoma / Q000201:enzymology*; D011854:Radiochemistry; D013619:Tacrine / Q000633:toxicity*; D013936:Thymidine / Q000378:metabolism; D013997:Time Factors; D014316:Tritium / Q000378:metabolism; D014407:Tumor Cells, Cultured
D002250:Carbon Radioisotopes; D014316:Tritium; D013619:Tacrine; D000535:Aluminum; D008995:Monoamine Oxidase; D007930:Leucine; D013936:Thymidine