title: Tissue engineered neocartilage using plasma derived polymer substrates and chondrocytes.
abstract: This study demonstrates that fibrin monomers can be polymerized into moldable gels and used for the encapsulation of isolated chondrocytes. This biologically derived scaffold will maintain three-dimensional spatial support, allowing new tissue development in a subcutaneous space. Chondrocytes isolated from the glenohumeral and humeroradioulnar joints of a calf were combined with cyroprecipitate and polymerized with bovine thrombin to create a fibrin glue gel with a final cell density of 12.5 x 10(6) cells/ml. The polymer-chondrocyte constructs were implanted subcutaneously in 12 nude mice and incubated for 6 and 12 weeks in vivo. Histologic and biochemical analysis including deoxyribonucleic acid (DNA) and glycosaminoglycan quantitation confirmed the presence of actively proliferating chondrocytes with production of a well-formed cartilaginous matrix in the transplanted samples. Control specimens from 12 implantation sites consisting of chondrocytes alone or fibrin glue substrates did not demonstrate any gross or histologic evidence of neocartilage formation. Moldable autogenous fibrin glue polymer systems have a potential to serve as alternatives to current proprietary polymer systems used for tissue engineering cartilage as well as autogenous grafts and alloplastic materials used for facial skeletal and soft-tissue augmentation.
authors.affiliation: Department of Surgery, Harvard Medical School, and Massachusetts General Hospital, Boston 02114, USA.
authors.forename: C D
authors.initials: CD
authors.lastname: Sims
authors.forename: P E
authors.initials: PE
authors.lastname: Butler
authors.forename: Y L
authors.initials: YL
authors.lastname: Cao
authors.forename: R
authors.initials: R
authors.lastname: Casanova
authors.forename: M A
authors.initials: MA
authors.lastname: Randolph
authors.forename: A
authors.initials: A
authors.lastname: Black
authors.forename: C A
authors.initials: CA
authors.lastname: Vacanti
authors.forename: M J
authors.initials: MJ
authors.lastname: Yaremchuk
mesh_terms: D000818:Animals; D001698:Biomedical Engineering; D002358:Cartilage, Articular / Q000254:growth & development*; D002417:Cattle; D019902:Chondrocytes / Q000637:transplantation*; D005260:Female; D015718:Fibrin Tissue Adhesive; D006801:Humans; D051379:Mice; D008819:Mice, Nude; D011108:Polymers
chemical_list: D015718:Fibrin Tissue Adhesive; D011108:Polymers