Release of endogenous glutamate and gamma-aminobutyric acid from rat striatal tissue slices measured by an improved method of high performance liquid chromatography with electrochemical detection.
The release of endogenous glutamate and gamma-aminobutyric acid (GABA) from rat brain tissue slices was studied using a tissue slice assay in which detectable amounts of the amino acids were released from 1-2 mg of tissue. An improved method of high performance liquid chromatography (HPLC) with electrochemical detection was employed to measure both glutamate and GABA after derivatization with o-phthalaldehyde and sulphite in a single isocratic HPLC analysis. The non-endogenous amino acid, homoglutamine, was used as an internal standard in verifying the consistent derivatization of amino acids and in quantifying amounts of glutamate and GABA released from the caudate-putamen tissue. The derivatized amino acids (1-30 pmol) were detected as chromatographic peaks eluting at baseline level and free of significant interfering co-eluates in a 25-30 min analysis time.
Phillips
J B
JB
Department of Pharmacology, Uniformed Services University of the Health Sciences, Bethesda, MD 20814, USA.
Cox
B M
BM
D000818:Animals; D002421:Caudate Nucleus / Q000166:cytology / Q000378:metabolism; D002851:Chromatography, High Pressure Liquid; D004563:Electrochemistry; D018698:Glutamic Acid / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D066298:In Vitro Techniques; D017072:Neostriatum / Q000378:metabolism*; D011188:Potassium / Q000494:pharmacology; D011699:Putamen / Q000166:cytology / Q000378:metabolism; D051381:Rats; D005680:gamma-Aminobutyric Acid / Q000378:metabolism*; D009764:o-Phthalaldehyde
D018698:Glutamic Acid; D005680:gamma-Aminobutyric Acid; D009764:o-Phthalaldehyde; D011188:Potassium