title: Human placenta and fetal membranes contain peptide YY1-36 and peptide YY3-36.
abstract: Extracts of human term amniotic, placental, and chorion/decidua tissue contained, respectively, 4.36 +/- 2.79 (pmol/g wet wt; mean +/- S.E.M.: n = 5). 2.78 +/- 0.5 (n = 5) and 0.68 +/- 0.68 (n = 5) peptide YY (PYY)-like immunoreactivity. Using a specific PYY antiserum, gel filtration chromatography and reverse-phase high performance liquid chromatography (HLPC), amniotic, placental and fetal intestinal tissue extracts were demonstrated to contain PYY-like immunoreactivity consisting of equal amounts of PYY1-36 and PYY3-36. The presence of pancreatic polypeptide was not detected in any of the extracts. Positive immunohistochemical staining for PYY was seen in extravillous trophoblasts in the decidual septa and fetal membranes, the syncytiotrophoblast and cytotrophoblasts, amniotic epithelial cells and in maternal decidual stromal cells. Positive staining for PYY was found at the earliest date examined (9.5 weeks) and remained present throughout pregnancy to term. PYY1-36 and PYY3-36 may play important roles in human pregnancy, acting via endocrine or paracrine mechanisms.
authors.affiliation: Department of Medicine, University of Western Ontario, London, Canada.
authors.forename: Q
authors.initials: Q
authors.lastname: Xiao
authors.forename: X
authors.initials: X
authors.lastname: Han
authors.forename: E
authors.initials: E
authors.lastname: Arany
authors.forename: D
authors.initials: D
authors.lastname: Hill
authors.forename: J R
authors.initials: JR
authors.lastname: Challis
authors.forename: T J
authors.initials: TJ
authors.lastname: McDonald
mesh_terms: D000650:Amnion / Q000737:chemistry; D002850:Chromatography, Gel; D002851:Chromatography, High Pressure Liquid; D003656:Decidua / Q000737:chemistry; D005321:Extraembryonic Membranes / Q000737:chemistry*; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D019894:Peptide YY / Q000032:analysis*; D010920:Placenta / Q000737:chemistry*; D011247:Pregnancy; D011863:Radioimmunoassay; D014327:Trophoblasts / Q000737:chemistry
chemical_list: D019894:Peptide YY