Effect of a low-calcium environment on EGF inducible AP-1 binding activity in osteoblastic MC3T3-E1 cells.
In the mouse osteoblastic cell line MC3T3-E1, the signaling response of DNA-binding protein induced by the treatment of epidermal growth factor (EGF) was examined using electrophoretic mobility shift assay. EGF increased the binding activity in nuclear extracts of MC3T3-E1 cells to TPA-responsive element (TRE). Competition experiments revealed that the binding activity to AP-1 site in the nuclear extracts of EGF treated MC3T3-E1 cells was entirely inhibited by unlabeled AP-1 consensus oligonucleotide. The DNA-binding activity of AP-1 by EGF in nuclear extracts of MC3T3-E1 cells cultured under a low calcium environment was more increased.
Deyama
A
A
Department of Oral Surgery II, Hokkaido University, Sapporo, Japan.
Deyama
Y
Y
Matsumoto
A
A
Okitsu
M
M
Yoshimura
Y
Y
Suzuki
K
K
Totsuka
Y
Y
D000818:Animals; D000831:Animals, Newborn; D001483:Base Sequence; D002118:Calcium / Q000494:pharmacology*; D002460:Cell Line; D002467:Cell Nucleus / Q000187:drug effects / Q000378:metabolism; D004815:Epidermal Growth Factor / Q000494:pharmacology*; D051379:Mice; D008810:Mice, Inbred C57BL; D015345:Oligonucleotide Probes; D010006:Osteoblasts / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D012886:Skull; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology; D018808:Transcription Factor AP-1 / Q000096:biosynthesis*
D015345:Oligonucleotide Probes; D018808:Transcription Factor AP-1; D004815:Epidermal Growth Factor; D013755:Tetradecanoylphorbol Acetate; D002118:Calcium