Quadruplex formation of oligonucleotides containing G clusters and their nuclease resistance.
Oligoribonucleotides containing four G clusters, (UGnU)4 (n = 3-5), were synthesized and characterized by electrophoretic analysis and resistance to nucleases. Electrophoretic analyses indicate that the oligomers except for (UG3U)4 can form antiparallel-stranded monomeric quadruplexes in the presence of potassium cation. The oligomers used in this study are protected from endonuclease P1 and venom phosphodiesterase degradation. In addition, the oligomers indicate resistance to nucleases in human serum and E. coli S-100 fraction.
Shida
T
T
Department of Applied Biology, Faculty of Textile Science and Technology, Shinshu University, Nagano, Japan.
Suda
M
M
Sekiguchi
J
J
D001482:Base Composition; D001483:Base Sequence; D004926:Escherichia coli / Q000378:metabolism; D006801:Humans; D006860:Hydrogen Bonding; D007700:Kinetics; D009838:Oligodeoxyribonucleotides / Q000097:blood / Q000737:chemistry* / Q000378:metabolism; D010727:Phosphoric Diester Hydrolases / Q000378:metabolism*; D015719:Single-Strand Specific DNA and RNA Endonucleases / Q000378:metabolism*
D009838:Oligodeoxyribonucleotides; D015719:Single-Strand Specific DNA and RNA Endonucleases; D010727:Phosphoric Diester Hydrolases