title: Expression of proliferating cell nuclear antigen in Wilms' tumor.
abstract: OBJECTIVE
  To investigate the expression of proliferating cell nuclear antigen (PCNA) and its clinical significance in Wilms' tumor.
  
  
  METHODS
  PCNA immunoreactivity on paraffin-embedded tissues was assessed retrospectively in 35 cases using labelled streptaridin biotin (LSAB) procedure, and its association with clinical and other biological features was studied.
  
  
  RESULTS
  Twelve tumors (34.29%) expressed PCNA. Expression rate increased with the advance of stage (P < 0.05). The S-phase fraction and proliferation index (PI) in children with PCNA positive expression were higher than those in children with negative PCNA expression (P < 0.05). The survival rate (16.67%) in patients with positive PCNA expression was significantly lower than that (73.91%) in no-expression ones (P < 0.01).
  
  
  CONCLUSIONS
  PCNA is a sensitive indicator for cell proliferation and is clinically useful in identifying a poor prognostic group of patients with Wilms' tumor.
authors.affiliation: Department of Pediatric Surgery, Xin Hua Hospital, Shanghai Second Medical University, China.
authors.forename: F
authors.initials: F
authors.lastname: Chen
authors.forename: G
authors.initials: G
authors.lastname: Liu
authors.forename: Z
authors.initials: Z
authors.lastname: Zhang
authors.forename: Z
authors.initials: Z
authors.lastname: Li
authors.forename: W
authors.initials: W
authors.lastname: Cai
mesh_terms: D002648:Child; D002675:Child, Preschool; D005260:Female; D005500:Follow-Up Studies; D006801:Humans; D007223:Infant; D008297:Male; D011379:Prognosis; D018809:Proliferating Cell Nuclear Antigen / Q000378:metabolism*; D012189:Retrospective Studies; D009396:Wilms Tumor / Q000378:metabolism / Q000473:pathology*
chemical_list: D018809:Proliferating Cell Nuclear Antigen