title: Purines and pyrimidines determination in urine using high-performance liquid chromatography.
abstract: Single purine and pyrimidine bases are involved in two fundamental metabolic pathways that lead to formation of the building stones of DNA and RNA. Purine and pyrimidine nucleotides are also critically important metabolites in many cellular functions. The main breakdown of purines and pyrimidines produces uric acid and B-minoacids, respectively. Therefore, the study of purine and pyrimidine compounds in body fluid has high clinical relevance. We report, in this work, our experience in purines and pyrimidines determination in urine from children presenting with a clinical picture suggesting an inborn these pathways.
authors.affiliation: Laboratory for the Study of Stand. Verif. Metabolic Disease, G. Gaslini Institute-University, Department of Pediatrics I, Genova, Italy.
authors.forename: G
authors.initials: G
authors.lastname: Minniti
authors.forename: U
authors.initials: U
authors.lastname: Caruso
authors.forename: R
authors.initials: R
authors.lastname: Cerone
authors.forename: E
authors.initials: E
authors.lastname: de Toni
mesh_terms: D015415:Biomarkers / Q000652:urine; D002648:Child; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D006801:Humans; D011686:Purine-Pyrimidine Metabolism, Inborn Errors / Q000145:classification / Q000175:diagnosis* / Q000652:urine*; D011687:Purines / Q000652:urine*; D011743:Pyrimidines / Q000652:urine*
chemical_list: D015415:Biomarkers; D011687:Purines; D011743:Pyrimidines