Partial protection of severe combined immunodeficient mice against infection with Babesia microti by in vitro-generated CD4+ T cell clones.
Because CD4+ T cells were considered to be involved in protection against infection with Babesia microti, specific CD4+ T cells were generated in vitro from recovered BALB/c mice and their protective activity was tested in vivo. The cells produced varying amounts of interferon (IFN)-gamma in vitro in response to parasite antigen. In passive transfer experiments, three out of eleven T cell clones tested exerted protective activity in the early phase after infection. However, there seemed to be no correlation between this protection and in vitro IFN-gamma production by the T cell clones. Although the protection was partial and short-lived, the result provided direct evidence that CD4+ T cells play a crucial role in defense against B. microti.
Hanafusa
Y
Y
Department of Veterinary Science, National Institute of Infectious Disease, Tokyo, Japan.
Onuma
M
M
Kamiyama
T
T
D019264:Adoptive Transfer; D000818:Animals; D000911:Antibodies, Monoclonal; D001404:Babesiosis / Q000150:complications / Q000276:immunology / Q000517:prevention & control*; D015496:CD4-Positive T-Lymphocytes / Q000187:drug effects / Q000276:immunology* / Q000637:transplantation; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology; D002478:Cells, Cultured; D002999:Clone Cells; D007371:Interferon-gamma / Q000096:biosynthesis*; D007376:Interleukin-2 / Q000494:pharmacology; D015847:Interleukin-4 / Q000096:biosynthesis; D051379:Mice; D008807:Mice, Inbred BALB C; D016513:Mice, SCID; D018512:Parasitemia / Q000276:immunology / Q000517:prevention & control; D011994:Recombinant Proteins / Q000494:pharmacology; D016511:Severe Combined Immunodeficiency / Q000150:complications / Q000276:immunology / Q000469:parasitology*; D013154:Spleen / Q000276:immunology
D000911:Antibodies, Monoclonal; D007376:Interleukin-2; D011994:Recombinant Proteins; D015847:Interleukin-4; D007371:Interferon-gamma