title: Markers of inflammation in crevicular fluid from peri-implant mucosa surrounding single crystal sapphire implants.
abstract: The 19 patients included in this study had all been successfully treated for total or partial edentulism with single crystal sapphire implants as retention for overdentures or fixed bridges. As there is a need for more reliable non-invasive parameters for detecting changes surrounding endosseous implants, the aim of this study was to assess the content and the activity of neutrophils in crevicular fluid samples from 3 categories of sites: (1) crevices around implants from edentulous patients (2) crevices around implants in partially edentulous patients, and (3) crevices surrounding teeth. Fluid samples were taken with paper strips from 9 partially edentulous and 10 edentulous patients and the volume measured with a Periotron 6000. Elastase activity was measured as a marker of neutrophil activity and lactoferrin concentration as a marker of the number of neutrophils. Elastase activity per microliter and lactoferrin concentration was, despite similar clinical and radiographic signs, significantly higher in samples from crevices surrounding teeth and implants in the partially edentulous patients compared to samples from crevices around implants in the totally edentulous patients. There were no differences between teeth and implants in partially edentulous patients. The increased elastase activity and lactoferrin concentration indicates a higher neutrophil activity in patients with remaining teeth.
authors.affiliation: Department of Prosthetic Dentistry, Faculty of Odontology, Karolinska Institutet, Stockholm, Sweden.
authors.forename: B
authors.initials: B
authors.lastname: Fartash
authors.forename: M
authors.initials: M
authors.lastname: Hultin
authors.forename: A
authors.initials: A
authors.lastname: Gustafsson
authors.forename: B
authors.initials: B
authors.lastname: Asman
authors.forename: K
authors.initials: K
authors.lastname: Arvidson
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000537:Aluminum Oxide; D015415:Biomarkers; D003758:Dental Implantation, Endosseous; D015921:Dental Implants / Q000009:adverse effects*; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D005883:Gingival Crevicular Fluid / Q000737:chemistry / Q000276:immunology*; D006801:Humans; D007576:Jaw, Edentulous, Partially / Q000276:immunology; D007781:Lactoferrin / Q000032:analysis; D007958:Leukocyte Count; D019272:Leukocyte Elastase / Q000032:analysis; D008297:Male; D008875:Middle Aged; D009066:Mouth, Edentulous / Q000276:immunology; D018375:Neutrophil Activation; D009504:Neutrophils / Q000378:metabolism; D010518:Periodontitis / Q000175:diagnosis* / Q000209:etiology / Q000276:immunology; D011506:Proteins / Q000032:analysis; D018709:Statistics, Nonparametric
chemical_list: D015415:Biomarkers; D015921:Dental Implants; D011506:Proteins; D007781:Lactoferrin; D019272:Leukocyte Elastase; D000537:Aluminum Oxide