Quick-FISH: a rapid fluorescence in situ hybridization technique for molecular cytogenetic analysis.
A rapid and simplified fluorescence in situ hybridization (FISH) technique for the detection of human chromosome-specific centromeric probes is described. Using chromosomes 1-, 4-, 11- and Y-specific fluorescence-labeled probes, the modified, or quick-FISH technique, was compared to two conventional FISH methods. The modified FISH technique detected human chromosomes without sacrificing sensitivity or signal quality in both fresh-frozen and paraffin-embedded tissue sections. Further studies demonstrated that this technique can be used with simultaneous application of dual-color probes. This novel technique offers the advantages of being simpler to perform and faster than conventional techniques. The quick-FISH technique can be substituted for any sensitive conventional FISH method for molecular cytogenetic analysis in fresh, fresh-frozen or paraffin-embedded tissues.
Banerjee
S K
SK
Molecular Gastroenterology & Pancreatic Cancer Research Unit, V.A. Medical Center, Kansas City, MO 64128-2226, USA.
Weston
A P
AP
Persons
D L
DL
Campbell
D R
DR
D000782:Aneuploidy; D002503:Centromere / Q000737:chemistry; D002869:Chromosome Aberrations / Q000175:diagnosis* / Q000235:genetics* / Q000473:pathology; D025063:Chromosome Disorders; D015342:DNA Probes; D005753:Gastric Mucosa / Q000737:chemistry / Q000473:pathology; D018628:Gene Dosage; D006801:Humans; D017404:In Situ Hybridization, Fluorescence / Q000379:methods*; D007399:Interphase / Q000235:genetics; D012680:Sensitivity and Specificity; D013997:Time Factors
D015342:DNA Probes