title: Effect of the lectins wheat germ agglutinin (WGA) and Ulex europaeus agglutinin (UEA-I) on the alpha-amylase secretion of rat pancreas in vitro and in vivo.
abstract: Lectins are able to bind to cholecystokinin (CCK) receptors and other glycosylated membrane proteins. The lectins wheat germ agglutinin (WGA) and Ulex europaeus agglutinin (UEA-I) are used for affinity chromatography to isolate the highly glycosylated CCK-A receptor of pancreatic acinar cells. According to the working hypothesis that lectin binding to the CCK receptor should alter the ligand-receptor interaction, the effect of WGA and UEA-I on CCK-8-induced enzyme secretion was studied on isolated rat pancreatic acini in vitro. In vitro both lectins showed a dosage-dependent inhibition of CCK-8-induced alpha-amylase secretion of acini over 60 min. WGA showed a strong inhibitory effect on amylase secretion, approximately 40%, in vitro. UEA-I caused a smaller, but significant decrease, approximately 20%, in enzyme secretion of isolated acini. Additionally, both lectins inhibited cerulein/secretin- or cerulein-induced pancreatic secretion of rats in vivo, but not after secretin alone. The results are discussed with respect to a possible influence of both lectins on the interaction of CCK or cerulein with the CCK-A receptor.
authors.affiliation: Department of Pathology, University of Rostock, Germany.
authors.forename: U
authors.initials: U
authors.lastname: Mikkat
authors.forename: I
authors.initials: I
authors.lastname: Damm
authors.forename: G
authors.initials: G
authors.lastname: Schröder
authors.forename: K
authors.initials: K
authors.lastname: Schmidt
authors.forename: C
authors.initials: C
authors.lastname: Wirth
authors.forename: H
authors.initials: H
authors.lastname: Weber
authors.forename: L
authors.initials: L
authors.lastname: Jonas
mesh_terms: D000818:Animals; D066298:In Vitro Techniques; D037102:Lectins / Q000494:pharmacology*; D008297:Male; D010179:Pancreas / Q000201:enzymology*; D037121:Plant Lectins; D051381:Rats; D011917:Rats, Inbred Lew; D012844:Sincalide / Q000494:pharmacology; D014909:Wheat Germ Agglutinins / Q000494:pharmacology*; D000516:alpha-Amylases / Q000378:metabolism*
chemical_list: D037102:Lectins; D037121:Plant Lectins; C036850:Ulex europaeus lectins; D014909:Wheat Germ Agglutinins; D000516:alpha-Amylases; D012844:Sincalide