title: A cosmid and cDNA fine physical map of a human chromosome 13q14 region frequently lost in B-cell chronic lymphocytic leukemia and identification of a new putative tumor suppressor gene, Leu5.
abstract: B-cell chronic lymphocytic leukemia (B-CLL) is a human hematological neoplastic disease often associated with the loss of a chromosome 13 region between RB1 gene and locus D13S25. A new tumor suppressor gene (TSG) may be located in the region. A cosmid contig has been constructed between the loci D13S1168 (WI9598) and D13S25 (H2-42), which corresponds to the minimal region shared by B-CLL associated deletions. The contig includes more than 200 LANL and ICRF cosmid clones covering 620 kb. Three cDNAs likely corresponding to three different genes have been found in the minimally deleted region, sequenced and mapped against the contigged cosmids. cDNA clone 10k4 as well as a chimeric clone 13g3, codes for a zinc-finger domain of the RING type and shares homology to some known genes involved in tumorigenesis (RET finger protein, BRCA1) and embryogenesis (MID1). We have termed the gene corresponding to 10k4/13g3 clones LEU5. This is the first gene with homology to known TSGs which has been found in the region of B-CLL rearrangements.
authors.affiliation: Vavilov Institute of General Genetics, Moscow, Russia.
authors.forename: B
authors.initials: B
authors.lastname: Kapanadze
authors.forename: V
authors.initials: V
authors.lastname: Kashuba
authors.forename: A
authors.initials: A
authors.lastname: Baranova
authors.forename: O
authors.initials: O
authors.lastname: Rasool
authors.forename: W
authors.initials: W
authors.lastname: van Everdink
authors.forename: Y
authors.initials: Y
authors.lastname: Liu
authors.forename: A
authors.initials: A
authors.lastname: Syomov
authors.forename: M
authors.initials: M
authors.lastname: Corcoran
authors.forename: A
authors.initials: A
authors.lastname: Poltaraus
authors.forename: V
authors.initials: V
authors.lastname: Brodyansky
authors.forename: N
authors.initials: N
authors.lastname: Syomova
authors.forename: A
authors.initials: A
authors.lastname: Kazakov
authors.forename: R
authors.initials: R
authors.lastname: Ibbotson
authors.forename: A
authors.initials: A
authors.lastname: van den Berg
authors.forename: R
authors.initials: R
authors.lastname: Gizatullin
authors.forename: L
authors.initials: L
authors.lastname: Fedorova
authors.forename: G
authors.initials: G
authors.lastname: Sulimova
authors.forename: A
authors.initials: A
authors.lastname: Zelenin
authors.forename: L
authors.initials: L
authors.lastname: Deaven
authors.forename: H
authors.initials: H
authors.lastname: Lehrach
authors.forename: D
authors.initials: D
authors.lastname: Grander
authors.forename: C
authors.initials: C
authors.lastname: Buys
authors.forename: D
authors.initials: D
authors.lastname: Oscier
authors.forename: E R
authors.initials: ER
authors.lastname: Zabarovsky
authors.forename: S
authors.initials: S
authors.lastname: Einhorn
authors.forename: N
authors.initials: N
authors.lastname: Yankovsky
mesh_terms: D000595:Amino Acid Sequence; D002872:Chromosome Deletion; D002874:Chromosome Mapping; D002882:Chromosomes, Human, Pair 13; D003360:Cosmids; D018076:DNA, Complementary; D004268:DNA-Binding Proteins / Q000235:genetics*; D016147:Genes, Tumor Suppressor; D006801:Humans; D015451:Leukemia, Lymphocytic, Chronic, B-Cell / Q000235:genetics*; D008969:Molecular Sequence Data; D025521:Tumor Suppressor Proteins; D016335:Zinc Fingers
chemical_list: D018076:DNA, Complementary; D004268:DNA-Binding Proteins; C112217:TRIM13 protein, human; D025521:Tumor Suppressor Proteins