Identification and location on syndecan-1 core protein of the epitopes of B-B2 and B-B4 monoclonal antibodies.
Using a phage display peptide library, we characterized the epitope of two monoclonal antibodies reacting with syndecan-1: B-B2 and B-B4. The identified epitopes QDIT, for B-B2, and LPEV, for B-B4, were found to align with residues 36-39 and 90-93 of the mature protein, respectively. In contrast to B-B4, the B-B2 epitope is close to a potential glycosaminoglycan attachment site. Since syndecan-1 is heavily glycosylated and post-translational modifications are cell type specific, these results might explain the differences observed in the reactivity pattern of B-B2 and B-B4 and suggest that these monoclonal antibodies are useful probes to study cell surface exposed syndecan-1.
Dore
J M
JM
Diaclone, 1 bvd. A. Fleming, Besançon, France. jmdore@diaclone.com
Morard
F
F
Vita
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N
Wijdenes
J
J
D000595:Amino Acid Sequence; D000911:Antibodies, Monoclonal / Q000276:immunology*; D000918:Antibody Specificity; D018604:Epitope Mapping; D008562:Membrane Glycoproteins / Q000276:immunology*; D008969:Molecular Sequence Data; D019151:Peptide Library; D010455:Peptides / Q000276:immunology; D011509:Proteoglycans / Q000276:immunology*; D011994:Recombinant Proteins; D053667:Syndecans
D000911:Antibodies, Monoclonal; D008562:Membrane Glycoproteins; D019151:Peptide Library; D010455:Peptides; D011509:Proteoglycans; D011994:Recombinant Proteins; D053667:Syndecans