Two-dimensional crystals of protein kinase C.
Three two-dimensional (2D) crystal forms of protein kinase C (PKC) alpha and three of PKC delta have been grown on lipid monolayers composed of dioleoylphosphatidylcholine: dioleoylphosphatidylserine: (45:50:5 molar ratio). In the absence of DO, two additional 2D crystals of PKC delta are seen, suggesting that the presence of diolein (DO) alters the conformation of intact PKC at the lipid surface. Reconstructions of electron micrographs of these eight lattices show good reproducibility and indicate that several are appropriate for three-dimensional reconstruction to 20 A resolution.
Owens
J M
JM
Department of Biology, University of Virginia, Charlottesville 22903-2477, USA.
Kretsinger
R H
RH
Sando
J J
JJ
Chertihin
O I
OI
D003460:Crystallization; D004075:Diglycerides; D007091:Image Processing, Computer-Assisted; D007527:Isoenzymes / Q000737:chemistry / Q000302:isolation & purification* / Q000648:ultrastructure*; D008854:Microscopy, Electron; D010713:Phosphatidylcholines; D010718:Phosphatidylserines; D011487:Protein Conformation; D011493:Protein Kinase C / Q000737:chemistry / Q000302:isolation & purification* / Q000648:ultrastructure*; D051571:Protein Kinase C-alpha; D051745:Protein Kinase C-delta; D011994:Recombinant Proteins / Q000737:chemistry / Q000302:isolation & purification / Q000648:ultrastructure; D013057:Spectrum Analysis; D014965:X-Rays
D004075:Diglycerides; D007527:Isoenzymes; D010713:Phosphatidylcholines; D010718:Phosphatidylserines; D011994:Recombinant Proteins; C042511:1,2-dioleoylphosphatidylserine; D011493:Protein Kinase C; D051571:Protein Kinase C-alpha; D051745:Protein Kinase C-delta; C017251:1,2-oleoylphosphatidylcholine; C013965:diolein