title: A P-type ATPase from the aquatic fungus Blastocladiella emersonii similar to animal Na,K-ATPases.
abstract: We have cloned a P-type ATPase gene from the aquatic fungus Blastocladiella emersonii (BePAT1) using a probe obtained with degenerate oligonucleotides, corresponding to two amino acid sequences highly conserved among all P-type ATPase isoforms, and the polymerase chain reaction technique. Nucleotide sequence analysis revealed a 3.4 kb open reading frame encoding a putative peptide of 1080 amino acid residues with a calculated molecular mass of 119 kDa, which presents all diagnostic features of P-type transporting ATPases. Comparison to other members of the family and phylogenetic analyses have shown that the BePAT1 protein belongs to the subfamily of Na,K- and H,K-ATPases, indicating that the divergence between the alpha-subunit of the Na,K-ATPase and other members of the P-type ATPase family has occurred before the divergence between the animal and fungal lineages in evolution.
authors.affiliation: Departamento de Bioquímica, Universidade de São Paulo, Brazil.
authors.forename: F S
authors.initials: FS
authors.lastname: de Souza
authors.forename: S L
authors.initials: SL
authors.lastname: Gomes
mesh_terms: D000251:Adenosine Triphosphatases / Q000235:genetics*; D000595:Amino Acid Sequence; D000818:Animals; D001754:Blastocladiella / Q000235:genetics*; D019143:Evolution, Molecular; D008969:Molecular Sequence Data; D016415:Sequence Alignment; D017421:Sequence Analysis; D017386:Sequence Homology, Amino Acid; D000254:Sodium-Potassium-Exchanging ATPase / Q000235:genetics*
chemical_list: D000251:Adenosine Triphosphatases; D000254:Sodium-Potassium-Exchanging ATPase