Effect of pertussis toxin on baclofen- and diphenhydramine-induced amnesia.
The effect of pretreatment with pertussis toxin at the doses of 0.25 and 0.50 microg per mouse ICV on the amnesic effect produced by baclofen (0.1 4 mg kg(-1) i.p.), diphenhydramine (15-30 mg kg(-1) i.p.) and scopolamine (0.5-5 mg kg(-1) i.p.) was investigated in the mouse passive avoidance test. Ten days after a single injection of pertussis toxin, baclofen (2 4 mg kg(-1) i.p.) amnesia was prevented. By contrast, pertussis toxin had no effect on diphenhydramine- and scopolamine-induced amnesia. Pretreatment with pertussis toxin at both doses used did not impair motor coordination of the mice, as revealed by the rota-rod test. The present results indicate that the activation of pertussis toxin-sensitive G-proteins represents an important transduction step in memory impairment induced by GABA(B) (gamma-aminobutyric acid B) agonists, but not by antihistaminic and antimuscarinic drugs.
Galeotti
N
N
Department of Preclinical and Clinical Pharmacology, University of Florence, Italy.
Ghelardini
C
C
Bartolini
A
A
D000647:Amnesia / Q000139:chemically induced / Q000517:prevention & control*; D000818:Animals; D001418:Baclofen / Q000633:toxicity*; D004155:Diphenhydramine / Q000633:toxicity*; D018755:GABA Agonists / Q000633:toxicity*; D019204:GTP-Binding Proteins / Q000187:drug effects / Q000502:physiology; D006634:Histamine H1 Antagonists / Q000633:toxicity*; D007276:Injections, Intraventricular; D008297:Male; D008568:Memory / Q000187:drug effects; D051379:Mice; D009048:Motor Skills / Q000187:drug effects; D037342:Pertussis Toxin; D010566:Virulence Factors, Bordetella / Q000627:therapeutic use*
D018755:GABA Agonists; D006634:Histamine H1 Antagonists; D010566:Virulence Factors, Bordetella; D004155:Diphenhydramine; D037342:Pertussis Toxin; D019204:GTP-Binding Proteins; D001418:Baclofen