[Generation and characterization of avian vitelline antibodies against lipopolysaccharide and lipid A. 2. Investigations of specificity of egg yolk antibodies (IgY) against endotoxin].
Various preparations of egg yolk antibodies against different endotoxins of gram-negative bacteria were characterized with regard to their immunological properties. To do this, we investigated the reactivity of antibodies against a number of lipopolysaccharides and lipid-A by enzyme-immuno-assays and immunoblot. It could be shown that all antibody preparations contained specific antibodies, reactive with their homologous antigen. Furthermore these antibodies showed cross-reactivity with structural diverse LPS- and lipid A-antigens from different sources. Anti lipid A-antibodies appeared to be highly crossreactive with purified LPS and lipid A from several gram-negative organisms. Egg yolk antibodies raised by immunization with LPS showed cross-reactivity with enterobacterial LPS and only marginal reactivity with both LPS from other gram-negative bacteria and lipid A. The results from immunoblot experiments confirmed our findings from EIA-studies.
Sasse
M
M
Institut für Virologie, Fachbereich Veterinärmedizin (Mitte), Freie Universität Berlin.
Hlinak
A
A
D000818:Animals; D000917:Antibody Formation; D000918:Antibody Specificity; D002645:Chickens / Q000276:immunology*; D003429:Cross Reactions; D004530:Egg Yolk / Q000276:immunology*; D004755:Enterobacteriaceae / Q000276:immunology; D006090:Gram-Negative Bacteria / Q000276:immunology*; D016905:Gram-Negative Bacterial Infections / Q000276:immunology / Q000517:prevention & control / Q000662:veterinary*; D015151:Immunoblotting; D007124:Immunoenzyme Techniques; D007136:Immunoglobulins / Q000096:biosynthesis* / Q000276:immunology; D008050:Lipid A / Q000276:immunology*; D008070:Lipopolysaccharides / Q000276:immunology*; D011201:Poultry Diseases
C016512:IgY; D007136:Immunoglobulins; D008050:Lipid A; D008070:Lipopolysaccharides
Herstellung und Charakterisierung aviärer vitelliner Antikörper gegen Lipopolysaccharide und Lipid A. 2. Mitteilung: Untersuchungen zur Spezifität von Dotterantikörpern (IgY) gegen Endotoxine.