Involvement of the RpoN protein in the transcription of the oprE gene in Pseudomonas aeruginosa.
OprE is a channel-forming outer membrane protein of Pseudomonas aeruginosa, the expression of which is induced under anaerobic conditions. We constructed various mutants and observed the effects on oprE expression. Deficiency in RpoN, an alternative sigma factor for RNA polymerase, abolished oprE expression under aerobic conditions, but did not affect the expression under anaerobic conditions. One mutation on the putative RpoN recognition site also caused reduction of oprE expression. The region 500 nucleotides upstream of the mRNA start site was required for optimal oprE transcription, which contains an AT-rich region including a putative integration host factor binding site. These results indicate that OprE production is directly or indirectly controlled by RpoN but also require some other regulatory proteins bound to the upstream region.
Yamano
Y
Y
Discovery Research Laboratories II, Shionogi Research Laboratories, Shionogi & Co., Ltd., Osaka, Japan. yoshinori.yamano@shionogi.co.jp
Nishikawa
T
T
Komatsu
Y
Y
D000332:Aerobiosis; D000693:Anaerobiosis; D001426:Bacterial Proteins; D001483:Base Sequence; D004268:DNA-Binding Proteins; D012321:DNA-Directed RNA Polymerases / Q000235:genetics / Q000502:physiology*; D015964:Gene Expression Regulation, Bacterial / Q000235:genetics*; D005798:Genes, Bacterial / Q000235:genetics; D008969:Molecular Sequence Data; D009154:Mutation; D018272:Porins / Q000235:genetics*; D011401:Promoter Regions, Genetic / Q000235:genetics; D011550:Pseudomonas aeruginosa / Q000235:genetics* / Q000378:metabolism; D051877:RNA Polymerase Sigma 54; D012808:Sigma Factor / Q000235:genetics / Q000502:physiology*; D014158:Transcription, Genetic / Q000235:genetics*
D001426:Bacterial Proteins; D004268:DNA-Binding Proteins; C082672:OprE protein, Pseudomonas; D018272:Porins; D012808:Sigma Factor; D012321:DNA-Directed RNA Polymerases; D051877:RNA Polymerase Sigma 54