Isolation of dsRNA-associated VLPs from the strain Cryptococcus hungaricus CBS 6569.
Double-stranded RNAs (dsRNAs) with molecular masses 1.7 and 5.0 kbp, respectively, were isolated from the strain Cryptococcus hungaricus CBS 6569. The dsRNAs were copurified with eicosahedric virus-like particles, 29 nm in diameter. This strain produced a protease-sensitive 'toxin' which inhibited the growth of strain C. hungaricus CBS 4214. The toxin had maximum activity at pH 3.7. The highest toxin amount was attained after a culture period of four days.
Pfeiffer
I
I
Department of Microbiology, Attila József University, Szeged, Hungary. pfeiffer@bio.u-szeged.hu
Gyantár
M
M
Kucsera
J
J
Párducz
A
A
D003454:Cryptococcus / Q000187:drug effects / Q000821:virology*; D019286:Endopeptidase K; D008970:Molecular Weight; D009183:Mycotoxins / Q000494:pharmacology; D012330:RNA, Double-Stranded / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D012367:RNA, Viral / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D014771:Virion / Q000302:isolation & purification / Q000648:ultrastructure
D009183:Mycotoxins; D012330:RNA, Double-Stranded; D012367:RNA, Viral; D019286:Endopeptidase K