[Endonucleolysis of chromatin in rat thymus and spleen lymphocytes after irradiation].
The level of soluble DNA fragments (polydesoxynucleotides) in thymus and spleen lymphocytes is enhanced 12 h after the whole-body X-ray irradiation in dose of 0.5 and 1 Gy. The level of Ca2+, Mg(2+)-DNAase activity in lysed cells extracts, measured with calf spleen DNA, was enhanced in the region of 0.1-0.5 mM CaCl2 in incubation medium and decreased at 5 mM CaCl2. Monitoring of DNA fragmentation by electrophoresis in agarose gels showed the presence of fragments with approximately 200, 400, 800 bp length. X-ray induced DNA fragmentation is supposed to be associated with Ca2+, Mg(2+)-endonuclease activation.
Solodushko
V O
VO
Taras Shevchenko State University, Kyiv, Ukraine.
Matyshevs'ka
O P
OP
Kucherenko
N Ie
NIe
D000818:Animals; D002843:Chromatin / Q000737:chemistry / Q000528:radiation effects*; D004247:DNA / Q000032:analysis; D053938:DNA Fragmentation / Q000528:radiation effects; D004587:Electrophoresis, Agar Gel; D004706:Endodeoxyribonucleases / Q000378:metabolism*; D004789:Enzyme Activation / Q000528:radiation effects; D009707:Nucleosomes / Q000528:radiation effects; D051381:Rats; D017208:Rats, Wistar; D013154:Spleen / Q000166:cytology / Q000528:radiation effects*; D013601:T-Lymphocytes / Q000201:enzymology / Q000528:radiation effects*; D013950:Thymus Gland / Q000166:cytology / Q000528:radiation effects*; D014916:Whole-Body Irradiation
D002843:Chromatin; D009707:Nucleosomes; D004247:DNA; D004706:Endodeoxyribonucleases; C029367:calcium magnesium dependent endodeoxyribonuclease
Endonukleoliz khromatynu u limfotsytakh selezinky ta tymusa shchuriv pislia oprominennia.