Spectrophotometric determination of thiols in human lymphocytes.
A spectroscopic method for thiol analysis, based on the complexation reaction with Pd(II), is described. The proposed method is simple and sensitive and can be used for a rapid analysis of thiols in human lymphocytes.
Raggi
M A
MA
Dipartimento di Scienze Farmaceutiche, Università di Bologna, Italy.
Schiavone
P
P
Mandrioli
R
R
Bugamelli
F
F
Frabetti
F
F
Marini
M
M
D002499:Centrifugation, Density Gradient; D005978:Glutathione / Q000097:blood; D006801:Humans; D007202:Indicators and Reagents; D007854:Lead / Q000737:chemistry; D008214:Lymphocytes / Q000737:chemistry*; D018384:Oxidative Stress / Q000502:physiology; D013053:Spectrophotometry; D013438:Sulfhydryl Compounds / Q000097:blood*
D007202:Indicators and Reagents; D013438:Sulfhydryl Compounds; D007854:Lead; D005978:Glutathione