title: [The preliminary study on prevention and treatment of neonatal jaundice].
abstract: OBJECTIVE
  The purification and identification of heme oxygenase isoforms (HO-1) and HO-2 from human liver were described and Sn-protoporphyrin (SnPP) was used to inhibit HO-1 activity in order to provide a new method for prevention and treatment of neonatal jaundice.
  
  
  METHODS
  Human hepatic microsomal fractions were purified by DEAE-Sephadex A-25 and hydroxylapatite chromatography. The enzyme activities of the two isoforms were detected with and without Snpp.
  
  
  RESULTS
  HO-1 was the predominant form with a ratio of 2.4:1 (HO-1:HO-2). The apparent molecular weight of HO-1 and HO-2 on SDS-PAGE was 30,000 and 36,000 under reducing conditions, respectively. The study also showed that HO-1 was the traditional inducible heme oxygenase and HO-2 was a non-inducible heme oxygenase.
  
  
  CONCLUSIONS
  The efficient inhibition effect of SnPP on HO-1 is suggested.
authors.affiliation: Ruijin Hospital Shanghai Second Medical University.
authors.forename: Z
authors.initials: Z
authors.lastname: Xia
authors.forename: S C
authors.initials: SC
authors.lastname: Yu
authors.forename: Y
authors.initials: Y
authors.lastname: Li
mesh_terms: D004791:Enzyme Inhibitors / Q000494:pharmacology; D006419:Heme Oxygenase (Decyclizing) / Q000037:antagonists & inhibitors / Q000378:metabolism*; D006801:Humans; D007231:Infant, Newborn; D007527:Isoenzymes / Q000378:metabolism*; D007567:Jaundice, Neonatal / Q000201:enzymology / Q000517:prevention & control*; D008099:Liver / Q000201:enzymology*; D008665:Metalloporphyrins / Q000494:pharmacology*; D008861:Microsomes; D011524:Protoporphyrins / Q000494:pharmacology*
chemical_list: D004791:Enzyme Inhibitors; D007527:Isoenzymes; D008665:Metalloporphyrins; D011524:Protoporphyrins; C032628:tin protoporphyrin IX; D006419:Heme Oxygenase (Decyclizing)