[Establishment of a novel culture system for specific expansion of human gamma delta T cell and study of its biological properties].
OBJECTIVE
To study the specific expansion method of human gamma delta T cell and the molecular mechanism against tumor cells.


METHODS
The peripheral blood mononuclear cells were co-cultured with XG-7 cells as stimulating cells and activated for the expansion of gamma delta T cells. Immunophonotype was analysed by IIFA and FACS. The cytotoxicity of gamma delta T cell against tumor cells was measured by 51Cr four hour release assay.


RESULTS
When this novel culture system was used, gamma delta T cells mainly expressing V gamma 9/V delta 2 encoded TCR were selected and expanded rapidly from 4.8 +/- 3.4% to 46.2 +/- 8.3%. With exogenous interleukin-2, gamma delta T cells were expanded in large quantity 10(10) within 3 weeks. gamma delta T cells mediated strong cytotoxic activity to different tumor cells including K562, Daudi, XG-7 cell lines, but no cytotoxic activity to normal cells was observed. XG-7 cells expressed HSP molecules and anti-HSP in mAB blocked the proliferation of gamma delta T cells.


CONCLUSIONS
The method shows unique biological properties: brief, rapid, well-repeating and wide cytotoxic activity against tumor cells. So gamma delta T cells are of value in tumor adoptive immunotherapy.
Li
X
X
Immunology Research Unit, Suzhou Medical College.
Zhang
X
X
Zhang
Y
Y
D002455:Cell Division; D002478:Cells, Cultured; D018920:Coculture Techniques; D003602:Cytotoxicity, Immunologic; D018840:HSP70 Heat-Shock Proteins / Q000378:metabolism; D006801:Humans; D007963:Leukocytes, Mononuclear; D016692:Receptors, Antigen, T-Cell, gamma-delta / Q000276:immunology*; D013601:T-Lymphocytes / Q000166:cytology* / Q000276:immunology; D014407:Tumor Cells, Cultured
D018840:HSP70 Heat-Shock Proteins; D016692:Receptors, Antigen, T-Cell, gamma-delta