title: Sulfoquinovosyldiacylglycerol, KM043, a new potent inhibitor of eukaryotic DNA polymerases and HIV-reverse transcriptase type 1 from a marine red alga, Gigartina tenella.
abstract: A new sulfolipid, KM043, which belongs to the 6-sulfo-alpha-D-quinovopyranosyl-(1-->3')-1',2'-diacylglycerol (SQDG) class of compounds, has been isolated from a marine red alga, Gigartina tenella, as a potent inhibitor of eukaryotic DNA polymerases and HIV-reverse transcriptase type 1. Its structure was determined on the basis of spectroscopic and gas chromatographic analyses. The inhibition was dose-dependent, and complete (more than 90%) inhibition of DNA polymerase alpha (pol. alpha), DNA polymerase beta (pol. beta) and HIV-reverse transcriptase type 1 (HIV-RT) was observed at concentrations of 5, 10, and 30 microM, respectively.
authors.affiliation: Department of Applied Biological Science, Faculty of Science and Technology, Science University of Tokyo, Japan.
authors.forename: K
authors.initials: K
authors.lastname: Ohta
authors.forename: Y
authors.initials: Y
authors.lastname: Mizushina
authors.forename: N
authors.initials: N
authors.lastname: Hirata
authors.forename: M
authors.initials: M
authors.lastname: Takemura
authors.forename: F
authors.initials: F
authors.lastname: Sugawara
authors.forename: A
authors.initials: A
authors.lastname: Matsukage
authors.forename: S
authors.initials: S
authors.lastname: Yoshida
authors.forename: K
authors.initials: K
authors.lastname: Sakaguchi
mesh_terms: D002849:Chromatography, Gas; D004075:Diglycerides / Q000302:isolation & purification; D006017:Glycolipids / Q000737:chemistry / Q000302:isolation & purification*; D054303:HIV Reverse Transcriptase / Q000037:antagonists & inhibitors*; D019384:Nucleic Acid Synthesis Inhibitors; D018894:Reverse Transcriptase Inhibitors / Q000737:chemistry / Q000302:isolation & purification*; D000461:Rhodophyta / Q000737:chemistry*; D016339:Spectrometry, Mass, Fast Atom Bombardment
chemical_list: D004075:Diglycerides; D006017:Glycolipids; D019384:Nucleic Acid Synthesis Inhibitors; D018894:Reverse Transcriptase Inhibitors; C013694:sulfoquinovosyl diglyceride; D054303:HIV Reverse Transcriptase