Novel detection system of flow injection analysis (1). The existence of significant relation between secondary structure of DNA and sensitivity in signal detection.
Polymerase Chain Reaction (PCR) products was detected quantitatively using a flow injection type sensor, based on Surface Plasmon Resonance (SPR). We used asymmetric PCR to amplify the two kinds of products; their DNA lengths are different. This novel design permitted us not only to detect PCR products with high-sensitivity, but also to develop a rapid DNA detection system for the sense of the genetic pathogen.
Sawata
S
S
National Space Development Agency of Japan, Ibaraki, Japan.
Kai
E
E
Ikebukuro
K
K
Iida
T
T
Honda
T
T
Karube
I
I
D001427:Bacterial Toxins / Q000235:genetics; D004247:DNA / Q000737:chemistry*; D004269:DNA, Bacterial / Q000032:analysis / Q000737:chemistry*; D004926:Escherichia coli / Q000235:genetics; D005798:Genes, Bacterial; D009690:Nucleic Acid Conformation; D016133:Polymerase Chain Reaction / Q000379:methods; D012680:Sensitivity and Specificity; D022641:Shiga Toxin 2; D013057:Spectrum Analysis / Q000379:methods
D001427:Bacterial Toxins; D004269:DNA, Bacterial; D022641:Shiga Toxin 2; D004247:DNA