Genome structure of pTi-SAKURA (II): genetic map constructed by complete DNA sequencing.
Ti plasmid (pTi-SAKURA) DNA isolated from an agrobacterium pathogenic against Japanese cherry trees were completely sequenced by primer walking with PCR subcloning. Typical genes including transfer DNA (T-DNA), nopaline utilizing genes, trb genes, traI, rep genes, tra genes, acc and vir genes were assigned in this order to pTi-SAKURA. Between the rep genes and tra genes, we found a large region which essentially lacks homology to any sequences in DNA databases. By amino acid sequence search, we could pick up several ORFs which are homologous with genes putatively capable to enhance interaction between agrobacteria and plants.
Suzuki
K
K
Department of Biological Science, Faculty of Science, Hiroshima University, Japan.
Hattori
Y
Y
Uraji
M
M
Ohta
N
N
Katoh
A
A
Yoshida
K
K
D016960:Agrobacterium tumefaciens / Q000235:genetics*; D000595:Amino Acid Sequence; D001483:Base Sequence; D003001:Cloning, Molecular; D017931:DNA Primers; D019992:Databases as Topic; D015723:Gene Library; D005798:Genes, Bacterial; D016680:Genome, Bacterial; D010957:Plasmids; D016133:Polymerase Chain Reaction / Q000379:methods; D016415:Sequence Alignment; D014197:Trees / Q000382:microbiology*
D017931:DNA Primers