title: A gridded genomic library of the honeybee (Apis mellifera): a reference library system for basic and comparative genetic studies of a hymenopteran genome.
abstract: We present a gridded genomic library of the honey-bee (Apis mellifera) for comparative and basic genetic study of the honeybee genome. The library will be established as a "Reference Library" system, and clones as well as data will be shared with the entire scientific community. This will accelerate the molecular level of honeybee genetics, combining the efforts of different laboratories. Because of male haploidy and the high rate of recombination, the honeybee is becoming a model organism for genomic studies of naturally occurring traits and behavioral genetics. The library consists of about 110,000 clones spotted at high density onto four filter membranes, representing 22 genome equivalents. Preliminary analysis using single-copy sequences revealed a positive clone number of the same order. The techniques for library generation and preliminary analysis as well as library access are described.
authors.affiliation: Institut für Okologie und Biologie, Technische Universität Berlin, Germany. beye0736@mailszrz.zrz.tu-berlin.de
authors.forename: M
authors.initials: M
authors.lastname: Beye
authors.forename: A
authors.initials: A
authors.lastname: Poch
authors.forename: C
authors.initials: C
authors.lastname: Burgtorf
authors.forename: R F
authors.initials: RF
authors.lastname: Moritz
authors.forename: H
authors.initials: H
authors.lastname: Lehrach
mesh_terms: D000818:Animals; D001516:Bees / Q000235:genetics*; D003001:Cloning, Molecular / Q000379:methods; D016208:Databases, Factual; D015698:Genomic Library; D006927:Hymenoptera / Q000235:genetics*; D009693:Nucleic Acid Hybridization