title: Changes in glycoconjugate expression of the sinus mucosa during experimental sinusitis: a lectin histochemical study of the epithelium and goblet cell development.
abstract: Lectin expression in both normal and inflamed sinus mucosa in rabbit was investigated histochemically. A different staining pattern was observed in the inflamed mucosa, i.e. the degree of staining reactivity with Canavalia ensiformis (ConA) decreased, whereas staining with Ulex europaeus agglutinin-I (UEA-I), Arachis hypoga (PNA) with neuraminidase pretreatment and Triticum vulgaris (WGA) intensified, indicating an enhanced fucosylation as well as sialylation. Goblet cells were stained with UEA-I, WGA, PNA and PNA with neuraminidase pretreatment, but scarcely with ConA. Positive PNA staining of basal cells and epithelial secretory granules was observed in the inflamed mucosa, especially close to areas where goblet cell development was prolific. It was therefore assumed that basal cells can differentiate into goblet cells-through epithelial secretory cells accompanied by sialylation and/or sulphonation of their mucins. It is concluded that the changes in glycoconjugate expression as well as goblet cell development in the inflamed mucosa are of importance for both local host resistance and defence mechanisms against microorganisms during the early stages of the inflammatory process.
authors.affiliation: Department of Oto-Rhino-Laryngology, Huddinge University Hospital, Karolinska Institute, Sweden.
authors.forename: N
authors.initials: N
authors.lastname: Otori
authors.forename: B
authors.initials: B
authors.lastname: Carlsöö
authors.forename: P
authors.initials: P
authors.lastname: Stierna
mesh_terms: D000818:Animals; D003208:Concanavalin A / Q000378:metabolism; D005260:Female; D006001:Glycoconjugates / Q000378:metabolism*; D006651:Histocytochemistry; D037102:Lectins / Q000378:metabolism; D008297:Male; D009297:Nasal Mucosa / Q000166:cytology / Q000378:metabolism*; D019887:Peanut Agglutinin / Q000378:metabolism; D037121:Plant Lectins; D011817:Rabbits; D012852:Sinusitis / Q000378:metabolism*; D013997:Time Factors; D014909:Wheat Germ Agglutinins / Q000378:metabolism
chemical_list: D006001:Glycoconjugates; D037102:Lectins; D019887:Peanut Agglutinin; D037121:Plant Lectins; C036850:Ulex europaeus lectins; D014909:Wheat Germ Agglutinins; D003208:Concanavalin A