Restricted usage of the T-cell receptor V beta repertoire in tonsillitis in association with palmoplantar pustulosis.
Focal infections such as chronic tonsillitis or dental caries occasionally play a role in the induction or exacerbation of palmoplantar pustulosis (PPP). Arthro-osteitis is sometimes a complication in severe cases of PPP. To study the effects of bacterial infection on the exacerbation of cutaneous lesions and arthralgia, we investigated the T-cell receptor V beta repertoire in peripheral blood mononuclear cells (PBMC) and tonsil tissue after tonsillectomy in 4 cases, who had chronic tonsillitis and a history of exacerbation of cutaneous lesions following a sore throat. First, serum levels of interleukin-6 (IL-6) and IL-8 were measured before and after tonsillectomy by enzyme-linked immunosorbent assay (ELISA). Second, 3H-TdR incorporation was used to examine the effects of the culture supernatant on the PBMC of the autologous patients, other PPP patients without tonsillitis and normal controls. T-cell receptor V beta repertoire was examined by the reverse transcriptase-polymerase chain reaction method. Results showed that IL-8 was significantly high in the serum and abundantly released from tonsillar lymphocytes, which may play a role in the accumulation of neutrophils in lesional skin. T-cell receptors V beta 6 and 12 were preferentially expressed on tonsillar lymphocytes, and V beta 4, 7, 9, 17 and 18 were detected relatively frequently. These data suggest that restricted usage of T-cell receptor V beta subsets may play a crucial role in the induction of tonsillitis associated with PPP.
Yamamoto
T
T
Department of Dermatology, Tokyo Medical and Dental University, School of Medicine, Japan.
Katayama
I
I
Nishioka
K
K
D000328:Adult; D017077:Culture Media, Conditioned / Q000737:chemistry / Q000494:pharmacology; D005260:Female; D015870:Gene Expression / Q000235:genetics; D006801:Humans; D015850:Interleukin-6 / Q000097:blood; D016209:Interleukin-8 / Q000097:blood; D007963:Leukocytes, Mononuclear / Q000187:drug effects; D008213:Lymphocyte Activation / Q000276:immunology; D008297:Male; D008875:Middle Aged; D008934:Mitogens / Q000276:immunology / Q000494:pharmacology; D014066:Palatine Tonsil / Q000166:cytology / Q000378:metabolism / Q000382:microbiology; D016133:Polymerase Chain Reaction; D011565:Psoriasis / Q000150:complications / Q000276:immunology* / Q000382:microbiology; D012313:RNA / Q000378:metabolism; D016693:Receptors, Antigen, T-Cell, alpha-beta / Q000235:genetics*; D013211:Staphylococcus aureus / Q000166:cytology / Q000302:isolation & purification; D018089:Superantigens / Q000502:physiology; D013601:T-Lymphocytes / Q000737:chemistry / Q000166:cytology / Q000276:immunology; D013997:Time Factors; D014068:Tonsillectomy; D014069:Tonsillitis / Q000150:complications / Q000276:immunology* / Q000601:surgery
D017077:Culture Media, Conditioned; D015850:Interleukin-6; D016209:Interleukin-8; D008934:Mitogens; D016693:Receptors, Antigen, T-Cell, alpha-beta; D018089:Superantigens; D012313:RNA