title: Thrombin-binding properties of thrombin aptamer derivatives.
abstract: We synthesized a single-stranded DNA with triple G-quartet structure and found that its thrombin-binding activity at 37 degrees C is as much as that of thrombin aptamer with double G-quartet structure which is a product of in vitro selection and amplification. This result suggests that the sequence motifs obtained by in vitro selection and amplification method might not be the only answer.
authors.affiliation: Research Center for Advanced Science and Technology, University of Tokyo, Japan.
authors.forename: K
authors.initials: K
authors.lastname: Sumikura
authors.forename: K
authors.initials: K
authors.lastname: Yano
authors.forename: K
authors.initials: K
authors.lastname: Ikebukuro
authors.forename: I
authors.initials: I
authors.lastname: Karube
mesh_terms: D001483:Base Sequence; D004277:DNA, Single-Stranded / Q000138:chemical synthesis / Q000737:chemistry / Q000378:metabolism; D007700:Kinetics; D009690:Nucleic Acid Conformation; D009838:Oligodeoxyribonucleotides / Q000138:chemical synthesis / Q000737:chemistry* / Q000378:metabolism; D011485:Protein Binding; D013816:Thermodynamics; D013917:Thrombin / Q000737:chemistry / Q000378:metabolism*
chemical_list: D004277:DNA, Single-Stranded; D009838:Oligodeoxyribonucleotides; D013917:Thrombin