The expression of Fos within the suprachiasmatic nucleus of the diurnal rodent Arvicanthis niloticus.
Rhythms in the expression of the nuclear phosphoprotein Fos, have been demonstrated in the suprachiasmatic nucleus (SCN) of nocturnal rodents. When rats are housed in a 12:12-h light/dark (LD) cycle the number of Fos-immunoreactive (-IR) cells within the SCN is higher during the day than at night [9,23]. In the two experiments reported here, Fos-IR was examined in the SCN of a diurnal murid rodent, Arvicanthis niloticus. First, thirty-six adult male A. niloticus housed in a 12:12-h LD cycle were perfused at six equally spaced time points beginning 1 h after lights on (n=6 per time point). Brains were sectioned and treated with immunohistochemical procedures for the identification of Fos. The number of Fos-IR cells in the SCN varied significantly as a function of time, and was highest 1 h after lights on and decreased thereafter. The distribution of Fos-IR within the SCN overlapped with that of arginine-vasopressin-IR (AVP-IR) and vasoactive intestinal peptide-IR (VIP-IR), but not with that of gastrin-releasing peptide-IR (GRP-IR). In the second study, double-labeling techniques revealed extensive Fos expression within SCN neurons containing AVP-IR, but not neurons containing GRP-IR. In conclusion, although the overall rhythm of Fos-IR in the SCN is similar in diurnal and nocturnal rodents, differences may exist with respect to the relative distribution of Fos-immunoreacte cells within different SCN cell populations.
Katona
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Department of Psychology and Neuroscience Program, Michigan State University, East Lansing, MI 48824-1117, USA.
Rose
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Smale
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D000818:Animals; D001127:Arginine Vasopressin / Q000032:analysis; D002940:Circadian Rhythm / Q000502:physiology*; D019886:Gastrin-Releasing Peptide / Q000032:analysis; D007150:Immunohistochemistry; D008297:Male; D009115:Muridae / Q000378:metabolism*; D009419:Nerve Tissue Proteins / Q000096:biosynthesis*; D016760:Proto-Oncogene Proteins c-fos / Q000096:biosynthesis*; D013493:Suprachiasmatic Nucleus / Q000378:metabolism*
D009419:Nerve Tissue Proteins; D016760:Proto-Oncogene Proteins c-fos; D001127:Arginine Vasopressin; D019886:Gastrin-Releasing Peptide