Genome diversity in temperate bacteriophages of Oenococcus oeni.
The genome structure of six bacteriophages of Oenococcus oeni was compared. Two distinct groups with no apparent restriction site conservation were defined. In members of the alpha group (fOgML34, fOg4029, fOg30 and fOg218) a 7.5 kb region containing the origin of DNA packaging (cos) was highly conserved. Stretches of DNA heterogeneity could also be assigned to particular regions and were mostly evident in the right area of the genomes. fOg44 and fOgPSU1 (beta group) were indistinguishable in the left half of their genomes, including cos, but were markedly dissimilar in other regions. Strong labelling signals detected in cross-hybridizations involving members of different groups were confined to fragments centrally located in their physical maps. The attachment site (attP) of fOg44 was assigned to this conserved region. It is suggested that recombination events at this location may have been important in generating the observed diversity of oenophage genomes.
Santos
R
R
Departamento de Biologia Vegetal, Faculdade de Ciências da Universidade de Lisboa, Portugal.
São-José
C
C
Vieira
G
G
Paveia
H
H
Santos
M A
MA
D001435:Bacteriophages / Q000235:genetics*; D003001:Cloning, Molecular; D004279:DNA, Viral; D014644:Genetic Variation; D016679:Genome, Viral; D006095:Gram-Positive Cocci / Q000821:virology; D007934:Leuconostoc / Q000821:virology; D009693:Nucleic Acid Hybridization; D015183:Restriction Mapping
D004279:DNA, Viral