Detection and isolation of a novel human gene located on Xp11.2-p11.4 that escapes X-inactivation using a two-dimensional DNA mapping method.
Using a two-dimensional DNA mapping method, we detected four NotI restriction sites that escape chromosome X-specific methylation in humans. Two genes corresponding to two of these sites that lie in the region of Xp11.2-p11.4 were cloned and their properties studied. One of the genes matched a known gene, but the other, termed EXLM1, is novel and is predominantly expressed in cultured lymphocytes and skeletal muscle.
Yoshikawa
H
H
Institute for Molecular and Cellular Biology, Osaka University, Japan.
Fujiyama
A
A
Nakai
K
K
Inazawa
J
J
Matsubara
K
K
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D015139:Blotting, Southern; D002461:Cell Line, Transformed; D003001:Cloning, Molecular; D019175:DNA Methylation; D018076:DNA, Complementary / Q000302:isolation & purification; D015252:Deoxyribonucleases, Type II Site-Specific / Q000378:metabolism; D004303:Dosage Compensation, Genetic; D015180:Electrophoresis, Gel, Two-Dimensional; D006801:Humans; D056892:Mediator Complex; D008969:Molecular Sequence Data; D009928:Organ Specificity; D015183:Restriction Mapping / Q000379:methods*; D017422:Sequence Analysis, DNA; D015534:Trans-Activators; D014158:Transcription, Genetic; D014960:X Chromosome / Q000235:genetics*
D018076:DNA, Complementary; C492160:MED14 protein, human; D056892:Mediator Complex; D015534:Trans-Activators; D015252:Deoxyribonucleases, Type II Site-Specific; C038565:GCGGCCGC-specific type II deoxyribonucleases