Membranes of herpes simplex virus type-1-infected human corneal epithelial cells are not permeabilized to macromolecules and therefore do not release IL-1alpha.
Nanogram amounts of the proinflammatory cytokine interleukin-1alpha (IL-1alpha) were detected in uninfected cultures of human corneal epithelial cells (HCEC). Although HSV-1 replicated >10(4)-fold in these cells and caused extensive cytopathic effects, virus infection was not accompanied by significant extracellular release of IL-1alpha. Additional studies showed that release of radiolabeled cytosolic proteins from virus-infected HCEC was no greater than that released by mock-infected cells. These findings indicate that HSV-1 infection of HCEC does not result in IL-1alpha release because newly formed virus progeny can escape infected cells without disrupting cell membranes.
Tran
M T
MT
Department of Microbiology/Immunology, College of Medicine, University of South Alabama, Mobile 36688, USA.
Dean
D A
DA
Lausch
R N
RN
Oakes
J E
JE
D002462:Cell Membrane / Q000502:physiology; D002463:Cell Membrane Permeability; D003315:Cornea / Q000378:metabolism / Q000502:physiology* / Q000821:virology*; D004847:Epithelial Cells / Q000378:metabolism / Q000502:physiology* / Q000821:virology*; D018259:Herpesvirus 1, Human / Q000502:physiology*; D006801:Humans; D007375:Interleukin-1 / Q000378:metabolism*; D007424:Intracellular Fluid; D046911:Macromolecular Substances
D007375:Interleukin-1; D046911:Macromolecular Substances