Evaluation of the analytical performance of an enzyme immunometric assay (EIA) designed to measure endogenous thyroid-stimulating hormone (TSH) in canine serum samples.
The analytical performance of a commercially available enzyme immunometric assay (EIA) designed to measure endogenous thyroid-stimulating hormone (cTSH) concentration in canine serum samples was evaluated. The precision of the assay was acceptable with intra- and inter-assay coefficients of variation (CV) of 5 and 11%, respectively. The assay maintained linearity under dilution and recovered added cTSH in a linear and proportional manner. The limit of detection (0.03 microgram/L) was well below the values measured in serum from dogs with primary hypothyroidism (median value 1.52 micrograms/L). Further, the assay clearly distinguished dogs with primary hypothyroidism from clinically healthy dogs (P = 0.0004). Based on these findings, it was concluded that the analytical performance of the assay was acceptable.
Iversen
L
L
Department of Clinical Studies, Royal Veterinary and Agricultural University, Frederiksberg, Denmark.
Høier
R
R
Jensen
A L
AL
Skydsgaard
M
M
Koch
J
J
D000818:Animals; D004283:Dog Diseases; D004285:Dogs / Q000097:blood*; D005260:Female; D007037:Hypothyroidism / Q000097:blood / Q000175:diagnosis / Q000662:veterinary*; D007124:Immunoenzyme Techniques / Q000662:veterinary; D008297:Male; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013972:Thyrotropin / Q000097:blood*
D013972:Thyrotropin