title: Electrostatic enhancement of diffusion-controlled protein-protein association: comparison of theory and experiment on barnase and barstar.
abstract: The electrostatic enhancement of the association rate of barnase and barstar is calculated using a transition-state theory like expression and atomic-detail modeling of the protein molecules. This expression predicts that the rate enhancement is simply the average Boltzmann factor in the region of configurational space where association occurs instantaneously in the diffusion-controlled limit. Based on experimental evidence, this "transition state" is defined by configurations in which, relative to the stereospecifically bound complex, the two proteins are shifted apart by approximately 8 A (so a layer of water can be accommodated in the interface) and the two binding surfaces are rotated away by 0 degrees to 3 degrees. The values of the average Boltzmann factor, calculated by solving the Poisson-Boltzmann equation, for the wild-type complex and 16 complexes with single mutations are found to correlate well with experimental results for the electrostatic rate enhancement. The predicted rate enhancement is found to be somewhat insensitive to the precise definition of the transition state, due to the long-range nature of electrostatic interactions. The experimental ionic strength dependence of the rate enhancement is also reasonably reproduced.
authors.affiliation: Department of Biochemistry, Hong Kong University of Science and Technology, Clear Water Bay, Kowloon, Hong Kong.
authors.forename: M
authors.initials: M
authors.lastname: Vijayakumar
authors.forename: K Y
authors.initials: KY
authors.lastname: Wong
authors.forename: G
authors.initials: G
authors.lastname: Schreiber
authors.forename: A R
authors.initials: AR
authors.lastname: Fersht
authors.forename: A
authors.initials: A
authors.lastname: Szabo
authors.forename: H X
authors.initials: HX
authors.lastname: Zhou
mesh_terms: D001426:Bacterial Proteins / Q000737:chemistry*; D004058:Diffusion; D007477:Ions; D008956:Models, Chemical; D008958:Models, Molecular; D011485:Protein Binding; D012260:Ribonucleases / Q000737:chemistry*; D055672:Static Electricity
chemical_list: D001426:Bacterial Proteins; D007477:Ions; C057438:barstar protein, Bacillus amyloliquefaciens; D012260:Ribonucleases; C019760:Bacillus amyloliquefaciens ribonuclease