title: [Potential role of gut peptides in stress-induced colonic motor disorder].
abstract: OBJECTIVE
  To explore the potential role of gut peptide in stress-induced colonic motor disorder.
  
  
  METHODS
  In 9 conscious Wistar rats pre-equipped with strain-gauge transducers on ascending and descending colon, colonic motility was recorded before, during and after stress. And colonic transit was evaluated by instilling Cr into the cecum through chronically implanted cannula in each group of 16 rats with or without stress, and then calculating the geometric center (GC) of radioactivity. The contents of VIP, SP, NT, SST, MOT and Leu-ENK in plasma, colonic mucosa and muscle layer were assessed in 16 stressed and 16 control rats. Exogenous peptides (VIP, SP, SST, NT) were intravenously administered in 9 rats to determine the colonic motor response. Also, the effects of peptides on colonic circular muscle strips were investigated.
  
  
  RESULTS
  Motor activity was increased after stress, whereas colonic transit was delayed. In the stressed rats, plasma levels of VIP and Leu-ENK were higher than those in controls. The content of Leu-ENK in muscle tissue decreased. Both in vivo and in vitro studies showed that SP and NT excited, whereas VIP and SST inhibited colonic motor activity.
  
  
  CONCLUSIONS
  Release of certain peptides is altered by stress. Increased release of ENK and VIP may be involved in stress-induced colonic motor disorder and in the regulation of "stress hormone" release.
authors.affiliation: Department of Gastroenterology, Peking Union Medical College Hospital, Beijing.
authors.forename: X
authors.initials: X
authors.lastname: Gui
authors.forename: G
authors.initials: G
authors.lastname: Pan
authors.forename: M
authors.initials: M
authors.lastname: Ke
mesh_terms: D000818:Animals; D003106:Colon / Q000503:physiopathology*; D004743:Enkephalin, Leucine / Q000378:metabolism; D005260:Female; D005768:Gastrointestinal Hormones / Q000378:metabolism*; D005772:Gastrointestinal Transit / Q000502:physiology*; D008297:Male; D051381:Rats; D017208:Rats, Wistar; D013312:Stress, Physiological / Q000378:metabolism*; D013373:Substance P / Q000378:metabolism; D014660:Vasoactive Intestinal Peptide / Q000378:metabolism
chemical_list: D005768:Gastrointestinal Hormones; D013373:Substance P; D014660:Vasoactive Intestinal Peptide; D004743:Enkephalin, Leucine