[Isolation of two fractions of hepatocytes and characterization of their lipid composition].
Two fractions of hepatocytes were isolated from the rat liver by nonfermentative method. These fractions were different for mechanical stability to the action of perfusion factors. It has been shown that cells of these fractions were differently separated in the linear density gradient of sucrose. It was connected with structural and functional heterogeneity of hepatocytes in the liver. Results obtained allow us to confirm that hepatocytes in the liver form at least two classes of cells with different lipid content. The plasmatic membranes of hepatocytes with high content of lipids were inclined to damage in the process of liver perfusion as compared to cells with low lipid content.
Sukach
A N
AN
Institute of Problems of Cryobiology and Cryomedicine, National Academy of Sciences of Ukraine, Kyiv.
Bozhkov
A I
AI
Menzianova
N G
NG
Skliar
A I
AI
Belous
A M
AM
D000818:Animals; D002462:Cell Membrane / Q000737:chemistry; D002469:Cell Separation; D002499:Centrifugation, Density Gradient; D002784:Cholesterol / Q000032:analysis; D002788:Cholesterol Esters / Q000032:analysis; D004075:Diglycerides / Q000032:analysis; D005227:Fatty Acids / Q000032:analysis; D005989:Glycerides / Q000032:analysis; D006498:Hepatectomy; D008099:Liver / Q000737:chemistry / Q000166:cytology*; D010743:Phospholipids / Q000032:analysis*; D051381:Rats; D014280:Triglycerides / Q000032:analysis; D014343:Trypan Blue
D002788:Cholesterol Esters; D004075:Diglycerides; D005227:Fatty Acids; D005989:Glycerides; D010743:Phospholipids; D014280:Triglycerides; D002784:Cholesterol; D014343:Trypan Blue
Vydelenie dvukh fraktsiĭ gepatotsitov i kharakteristika ikh lipidnogo sostava.