title: Conformational distortion of sugar moieties in the RecA-bound DNA structure determined by a simulated annealing method.
abstract: We recently reported an NMR study indicating that the DNA structure bound to RecA protein contains a novel deoxyribose-base stacking interaction. Although the final structure calculated by the simulated annealing was well defined, the type of sugar puckers was neither pure C2'-endo nor C3'-endo, but something in between. This implies that the sugar moieties in the RecA-bound state actually take an intermediate puckering, but we can not exclude the possibility that RecA-bound DNA takes multiple conformations between the S-type and N-type of sugar puckers. In either case, the DNA structure within RecA filaments shows a unique property that is distinct from canonical DNA structures.
authors.affiliation: Cellular and Molecular Biology Laboratory, Saitama, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Nishinaka
authors.forename: Y
authors.initials: Y
authors.lastname: Ito
authors.forename: S
authors.initials: S
authors.lastname: Yokoyama
authors.forename: T
authors.initials: T
authors.lastname: Shibata
mesh_terms: D004247:DNA / Q000737:chemistry* / Q000378:metabolism*; D003855:Deoxyribose; D019906:Nuclear Magnetic Resonance, Biomolecular; D009690:Nucleic Acid Conformation; D009838:Oligodeoxyribonucleotides / Q000737:chemistry*; D011938:Rec A Recombinases / Q000737:chemistry* / Q000378:metabolism*; D013314:Stress, Mechanical
chemical_list: D009838:Oligodeoxyribonucleotides; D003855:Deoxyribose; D004247:DNA; D011938:Rec A Recombinases