Inhibitory effect of a marine microalgal polysaccharide on the telomerase activity in K562 cells.
An extracellular polysaccharide from marine microalga, dinoflagellate Gymnodinium sp. A3 (GA3), showed cytotoxicity to human myeloid leukemia K562 cells. We measured telomerase activity in K562 cells cultured with GA3 polysaccharide. 10.0 micrograms/ml of GA3 polysaccharide inhibited the telomerase activity in the cells completely. Also, we found a decrease in expression of the catalytic subunit of protein phosphatase (PP) type 1, PP1 gamma 1, in K562 cells cultured with GA3 polysaccharide.
Sogawa
K
K
First Department of Surgery, University of Tokushima School of Medicine, Japan.
Sumida
T
T
Hamakawa
H
H
Yamada
T
T
Matsumoto
K
K
Matsuda
M
M
Oda
H
H
Miyake
H
H
Tashiro
S
S
Okutani
K
K
D000818:Animals; D004141:Dinoflagellida / Q000737:chemistry*; D004791:Enzyme Inhibitors / Q000494:pharmacology*; D004794:Enzyme Repression; D006801:Humans; D007150:Immunohistochemistry; D010749:Phosphoprotein Phosphatases / Q000032:analysis / Q000378:metabolism; D011134:Polysaccharides / Q000494:pharmacology*; D019098:Telomerase / Q000037:antagonists & inhibitors* / Q000096:biosynthesis; D014407:Tumor Cells, Cultured / Q000187:drug effects / Q000201:enzymology
D004791:Enzyme Inhibitors; D011134:Polysaccharides; D019098:Telomerase; D010749:Phosphoprotein Phosphatases