title: Survey of Klebsiella pneumoniae strains producing extended-spectrum beta-lactamases: prevalence of SHV-12 and SHV-2a in Korea.
abstract: Fifty-three clinical isolates of extended-spectrum beta-lactamase (ESBL)-producing Klebsiella pneumoniae collected from three hospitals in Korea were investigated for phenotypical and genotypical characterizations. Among these, 39 strains (74%) were shown by isoelectric focusing to carry SHV-type beta-lactamases: 27 strains showed the pI 8.2 beta-lactamase, and another 12 strains showed the pI 7.6 beta-lactamase. The SHV gene of each of these strains was amplified by PCR, followed by nucleotide sequencing analysis. The gene of the pI 8.2 beta-lactamase was found to be identical to the sequences encoding SHV-12, and the gene of the pI 7.6 beta-lactamase was identical to the sequences encoding SHV-2a. A total of eight cefoxitin-resistant strains were found to have the plasmid-mediated AmpC-type beta-lactamase, with a pI of 8.0, and this was confirmed to be CMY-1 beta-lactamase by PCR and hybridization analysis. Noteworthy in this study is the fact that SHV-12 and SHV-2a have been the most commonly identified SHV-type ESBLs in Korea.
authors.affiliation: Department of Microbiology, College of Medicine, Dankook University, Chonan, Chungnam, Korea. minkim@ns.anseo.dankook.ac.kr
authors.forename: J
authors.initials: J
authors.lastname: Kim
authors.forename: Y
authors.initials: Y
authors.lastname: Kwon
authors.forename: H
authors.initials: H
authors.lastname: Pai
authors.forename: J W
authors.initials: JW
authors.lastname: Kim
authors.forename: D T
authors.initials: DT
authors.lastname: Cho
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology; D005838:Genotype; D006801:Humans; D007525:Isoelectric Focusing; D007711:Klebsiella pneumoniae / Q000187:drug effects / Q000201:enzymology*; D007723:Korea; D008826:Microbial Sensitivity Tests; D009154:Mutation; D010641:Phenotype; D018440:beta-Lactam Resistance / Q000235:genetics; D001618:beta-Lactamases / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism
chemical_list: D000900:Anti-Bacterial Agents; D001618:beta-Lactamases