Determination of p-hydroxyphenylglycine by reaction with o-phthalaldehyde using a flow-injection fluorimetric procedure.
In the present study, new flow injection procedures for the determination of p-hydroxyphenylglycine using either photometric or fluorimetric detection are proposed. The methods are based on the reaction of the amino acid with o-phthalaldehyde and 2-mercaptoethanol. The calibration graphs based on peak area were linear in the ranges 20-300 ng ml-1 with fluorimetric detection and 5-60 micrograms ml-1 using the photometric mode. The detection limit calculated for the fluorimetric procedure was 10 ng ml-1. The method was applied to the determination of the free p-hydroxyphenylglycine present in industrial pharmaceutical samples of a Dane salt.
Viñas
P
P
Department of Analytical Chemistry, Faculty of Chemistry, University of Murcia, Spain.
Campillo
N
N
López-Erroz
C
C
Hernández-Córdoba
M
M
D017022:Flow Injection Analysis; D005470:Fluorometry; D005998:Glycine / Q000031:analogs & derivatives* / Q000032:analysis; D007202:Indicators and Reagents; D008623:Mercaptoethanol; D009764:o-Phthalaldehyde
D007202:Indicators and Reagents; D008623:Mercaptoethanol; D009764:o-Phthalaldehyde; C023010:4-hydroxyphenylglycine; D005998:Glycine