title: HPLC-fluorescence determination of chlorocresol and chloroxylenol in pharmaceuticals.
abstract: The use of 2-chloro-6,7-dimethoxy-3-quinolinecarboxaldehyde as a fluorogenic labelling reagent in pre-column derivatization for the HPLC separation of chlorophenols has been investigated. The compound reacts (50 min at 110 degrees C) with 2- and 4-chlorophenols to give fluorescent ethers that can be separated by reversed-phase HPLC and detected at lambda exc = 360 nm, lambda em = 500 nm. The experimental conditions for derivatization and chromatographic separation are discussed. Applications for the determination of chlorocresol (4-chloro-3-cresol) and chloroxylenol (4-chloro-3,5-xylenol) in pharmaceutical formulations (creams, ointments) are described.
authors.affiliation: Dipartimento di Scienze Farmaceutiche, Università di Bologna, Italy.
authors.forename: R
authors.initials: R
authors.lastname: Gatti
authors.forename: P
authors.initials: P
authors.lastname: Roveri
authors.forename: D
authors.initials: D
authors.lastname: Bonazzi
authors.forename: V
authors.initials: V
authors.lastname: Cavrini
mesh_terms: D002138:Calibration; D002851:Chromatography, High Pressure Liquid; D003408:Cresols / Q000032:analysis*; D005456:Fluorescent Dyes; D009682:Magnetic Resonance Spectroscopy; D009824:Ointments; D011804:Quinolines; D013050:Spectrometry, Fluorescence; D013055:Spectrophotometry, Infrared; D014992:Xylenes / Q000032:analysis*
chemical_list: C113024:2-chloro-6,7-dimethoxy-3-quinolinecarboxaldehyde; D003408:Cresols; D005456:Fluorescent Dyes; D009824:Ointments; D011804:Quinolines; D014992:Xylenes; C007027:chloroxylenol; C006984:chlorocresol