[Development of an enzyme assay for detection of hepatitis B virus core IGM antibodies].
Detection of IgM anti-core (anti-HBcAg) antibodies of Hepatitis B Virus (HBV) is an useful marker for hepatitis B virus (HBV) acute infection. The aim of this study was to perform an immunodiagnostic assay for the detection of IgM anti-HBcAg antibodies. Hepatitis B core antigen (HBcAg) was produced by a recombinant clone of Escherichia coli and used for the development of the immunoassay. An IgM capture enzyme immunoassay (EIA) was selected for the detection of IgM anti-HBcAg antibodies. A total of 110 human plasma or sera were tested by the capture EIA and a commercial assay. The capture EIA yielded 99% of sensitivity and 93% specificity, when compared with the commercial test. The capture EIA developed here is of interest for epidemiological studies, particularly for endemic regions in South America.
Uzcátegui
N
N
Laboratorio de Biología de Virus, Universidad Central de Venezuela, Caracas, Venezuela.
Flores
J
J
Liprandi
F
F
Pujol
F H
FH
D015153:Blotting, Western; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D006509:Hepatitis B / Q000175:diagnosis; D006510:Hepatitis B Antibodies / Q000032:analysis*; D006512:Hepatitis B Core Antigens / Q000276:immunology*; D006801:Humans; D007075:Immunoglobulin M / Q000032:analysis*; D012680:Sensitivity and Specificity
D006510:Hepatitis B Antibodies; D006512:Hepatitis B Core Antigens; D007075:Immunoglobulin M
Desarrollo de un ensayo enzimático para la determinación de anticuerpos IgM anti-cápside del virus de la Hepatitis B.