-GG- specific cleavage via hole hopping through double-stranded DNA.
We examined the photoinduced DNA cleavage reaction of 32P-end labeled oligodeoxyribonucleotides which contain 5'-TGGGC-3', 5'-CGGGC-3', and 5'-TGGGT-3'. As for 5'-TGGGC-3' and 5'-TGGGT-3', the cleavage occurred most strongly at the middle of 5'-GGG-3' step, whereas the strongest cleavage occurred at the 5' side of 5'-GGG-3' in 5'-CGGGC-3' step. These results showed the 5' side base of 5'-GGG-3' step was very important for the sequence selectivity of 5'-GGG-3' cleavage.
Nakamura
T
T
Department of Synthetic Chemistry and Biological Chemistry, Faculty of Engineering, Kyoto University, Japan.
Dohno
C
C
Nakatani
K
K
Saito
I
I
D001483:Base Sequence; D004247:DNA / Q000737:chemistry*; D004249:DNA Damage; D015226:Dinucleoside Phosphates / Q000737:chemistry*; D009838:Oligodeoxyribonucleotides / Q000737:chemistry*; D010084:Oxidation-Reduction
D015226:Dinucleoside Phosphates; D009838:Oligodeoxyribonucleotides; C018309:guanylyl-(3'-5')-guanosine; D004247:DNA