title: DNA damage in isolated rat hepatocytes exposed to C.I. pigment orange 5 and C.I. pigment yellow 12 by the alkaline comet assay.
abstract: The induction of DNA damage by commonly used printing ink pigments, C.I. pigment orange 5 (C.I. 12075) and C.I. pigment yellow 12 (C.I. 21090), was investigated in freshly isolated rat hepatocytes with the comet assay. C.I. pigment yellow 12 is a 3,3'-dichlorobenzidine-based diarylide pigment, and C.I. pigment orange 5 is a naphthol-azo pigment. The pigments are virtually insoluble in aqueous solutions, and they have not been tested extensively for toxicological effects. C.I. pigment orange 5 increased the levels of DNA damage at 5 microg/ml (P < 0.02) and C.I. pigment yellow 12 at 20 microg/ml (P < 0.002). The effect of incubation time (20, 40, and 80 min) of the same concentrations of the pigments was tested. The levels of DNA damage were increased up to 80 min. Both pigments produced DNA damage that was in the same range as the food carcinogen 2-amino-3,8-dimethylimidazo[4,5-f]quinoxaline. Our data indicate that both C.I. pigment orange 5 and C.I. pigment yellow 12 are genotoxic in hepatocytes with metabolizing capacities. However, further investigation of the metabolism and disposition are required for the evaluation of the safety of these pigments.
authors.affiliation: National Institute of Occupational Health, Copenhagen, Denmark. pm@ami.dk
authors.forename: P
authors.initials: P
authors.lastname: Møller
authors.forename: H
authors.initials: H
authors.lastname: Wallin
authors.forename: N
authors.initials: N
authors.lastname: Grunnet
authors.forename: L
authors.initials: L
authors.lastname: Risom
authors.forename: L E
authors.initials: LE
authors.lastname: Knudsen
mesh_terms: D000468:Alkalies; D000818:Animals; D001391:Azo Compounds / Q000633:toxicity*; D001711:Biotransformation; D002469:Cell Separation; D004396:Coloring Agents / Q000633:toxicity*; D004249:DNA Damage; D004305:Dose-Response Relationship, Drug; D004586:Electrophoresis / Q000379:methods; D005260:Female; D008099:Liver / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D009152:Mutagenicity Tests; D011810:Quinoxalines / Q000633:toxicity; D051381:Rats; D017208:Rats, Wistar
chemical_list: D000468:Alkalies; D001391:Azo Compounds; D004396:Coloring Agents; D011810:Quinoxalines; C036990:2-amino-3,8-dimethylimidazo(4,5-f)quinoxaline; C045949:1-((2,4-dinitrophenyl)azo)-2-naphthalenol; C024907:pigment yellow 12