Evidence for PPC1, a determinant of the pilei-pellis color of Agaricus bisporus fruitbodies.
In the present study, we investigated the genetic basis of mushroom cap color. In first generation hybrids between a brown isolate and the white commercial hybrid U 1, the white trait was recessive. Color was determined using color meter technology in second generation hybrids obtained by crossing the homokaryotic progeny of a first generation hybrid with a homokaryon from U 1. Statistical analysis revealed a bimodal distribution describing two classes of white and not-white hybrids. We postulate that a recessive allele at a single locus (PPC1) encodes the white pilei-pellis color. Joint segregation analyses indicated that PPC1 was linked to the ADH (alcohol dehydrogenase) locus. Through the analysis of the heterokaryotic progeny of the first generation hybrid, a recombination model is proposed in which PPC1 is located between the centromere and the ADH locus.
Callac
P
P
Station de Recherches sur les Champignons, Institut National de la Recherche Agronomique, Cedex, France.callac@bordeaux.inra.fr
Moquet
F
F
Imbernon
M
M
Guedes-Lafargue
M R
MR
Mamoun
M
M
Olivier
J M
JM
D000364:Agaricus / Q000235:genetics*; D000426:Alcohol Dehydrogenase / Q000235:genetics; D000483:Alleles; D005787:Gene Frequency; D005800:Genes, Fungal; D005808:Genes, Recessive; D008040:Genetic Linkage; D005838:Genotype; D008540:Meiosis; D010858:Pigmentation / Q000235:genetics*; D011995:Recombination, Genetic
D000426:Alcohol Dehydrogenase