title: Identification of Btr-regulated genes using a titration assay. Search for a role for this transcriptional regulator in the growth and virulence of Bordetella pertussis.
abstract: Bordetella pertussis is the causative agent of the respiratory disease pertussis or whopoping cough. Btr, an oxygen-responsive transcriptional regulator of B. pertussis, is homologous to the FNR protein of E. coli. Using a murine respiratory model, we observed in the present study that Btr is important in growth and survival of B. pertussis in vivo. A titration assay was developed that identified genes containing Btr binding sites including B. pertussis sodB and btr, E. coli aspA and a new B. pertussis gene, brg1. The brg1 gene encodes a protein similar to the LysR family of transcriptional regulators and its expression is activated threefold by Btr under anaerobic growth conditions but unaffected by Btr aerobically. The nucleotide sequence flanking brg1 encodes proteins with similarity to various metabolic enzymes. Putative overlapping promoters and a Btr binding site (FNR box) were identified in the DNA sequence between brg1 and the adjacent genes. These intervening sequences may represent sites for regulation by Btr and Brg1.
authors.affiliation: University of Arizona, Health Sciences Center, Tucson, AZ 85724, USA.
authors.forename: G E
authors.initials: GE
authors.lastname: Wood
authors.forename: N
authors.initials: N
authors.lastname: Khelef
authors.forename: N
authors.initials: N
authors.lastname: Guiso
authors.forename: R L
authors.initials: RL
authors.lastname: Friedman
mesh_terms: D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000096:biosynthesis / Q000235:genetics* / Q000378:metabolism*; D001483:Base Sequence; D001886:Bordetella pertussis / Q000235:genetics* / Q000254:growth & development / Q000472:pathogenicity; D004268:DNA-Binding Proteins; D004926:Escherichia coli / Q000235:genetics; D015964:Gene Expression Regulation, Bacterial; D015698:Genomic Library; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D011401:Promoter Regions, Genetic; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis; D015183:Restriction Mapping; D013482:Superoxide Dismutase / Q000096:biosynthesis / Q000235:genetics*; D014157:Transcription Factors; D014774:Virulence
chemical_list: C084167:BTR protein, Bordetella pertussis; D001426:Bacterial Proteins; D004268:DNA-Binding Proteins; D011993:Recombinant Fusion Proteins; D014157:Transcription Factors; C087504:SodB protein, Bacteria; D013482:Superoxide Dismutase