Inhibitory effect of shofu-san, a Japanese kampo medicine, on neutrophil functions in vitro.
The effect of Shofu-san (TJ-22), an oral Japanese kampo medicine, on several inflammatory parameters of human neutrophils, including reactive oxygen species (ROS) generation, chemotaxis, and calcium concentration, [Ca2+]i in neutrophils was examined in vitro. In addition, ROS generated in a cell-free, xanthine-xanthine oxidase system was also assessed. The species investigated were superoxide radical anion (O2-), hydrogen peroxide (H2O2), and hydroxyl radical (OH.). TJ-22 significantly decreased neutrophil-generated O2-, H2O2 and OH. in a dose-dependent manner. The medicine also markedly inhibited neutrophil chemotaxis and [Ca2+]i in neutrophils. In addition, three kinds of ROS generated in the cell-free system was reduced in the presence of TJ-22. Our results indicate that TJ-22 may exert an anti-inflammatory action by inhibiting the neutrophil functions and scavenging ROS, leading to a reduction in oxidative tissue injury.
Akamatsu
H
H
Department of Dermatology, Kansai Medical University, Osaka, Japan.
Asada
Y
Y
Horio
T
T
D002118:Calcium / Q000378:metabolism; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D006801:Humans; D006861:Hydrogen Peroxide / Q000378:metabolism; D017665:Hydroxyl Radical / Q000378:metabolism; D007564:Japan; D008516:Medicine, Chinese Traditional; D009504:Neutrophils / Q000187:drug effects* / Q000378:metabolism / Q000502:physiology*; D013481:Superoxides / Q000378:metabolism
D004365:Drugs, Chinese Herbal; D013481:Superoxides; D017665:Hydroxyl Radical; D006861:Hydrogen Peroxide; D002118:Calcium