title: Expression of Xenopus laevis translation initiation factor 4E (eIF-4E) by baculovirus-insect cell system.
abstract: A gene encoding Xenopus laevis eIF-4E was cloned into a transfer vector, and its gene expression was attempted in cells of E. coli, yeast and insect. Effective expression of the active eIF-4E was achieved in the soluble fraction of the insect cell Sf9, which was infected with the recombinant baculovirus. Overexpression of the eIF-4E protein caused remarkable change in the shape of the cells.
authors.affiliation: Institute for Biomolecular Science, Gakushuin University, Tokyo, Japan.
authors.forename: H
authors.initials: H
authors.lastname: Miyoshi
authors.forename: K
authors.initials: K
authors.lastname: Ito
authors.forename: N
authors.initials: N
authors.lastname: Sakai
authors.forename: J
authors.initials: J
authors.lastname: Mizushima
authors.forename: K
authors.initials: K
authors.lastname: Okamoto
authors.forename: H
authors.initials: H
authors.lastname: Hori
authors.forename: T
authors.initials: T
authors.lastname: Nishino
authors.forename: M
authors.initials: M
authors.lastname: Wakiyama
authors.forename: K
authors.initials: K
authors.lastname: Miura
mesh_terms: D000818:Animals; D016367:Baculoviridae; D002460:Cell Line; D039561:Eukaryotic Initiation Factor-4E; D007700:Kinetics; D010448:Peptide Initiation Factors / Q000096:biosynthesis*; D011994:Recombinant Proteins / Q000096:biosynthesis; D018411:Spodoptera / Q000166:cytology; D014162:Transfection; D014982:Xenopus laevis
chemical_list: D039561:Eukaryotic Initiation Factor-4E; D010448:Peptide Initiation Factors; D011994:Recombinant Proteins