Differential interactions of MAP2, tau and MAP5 during axogenesis in culture.
The subcellular interactions of the neuronal microtubule-associated proteins tau, MAP2 and MAP5 were determined in cultured rat hippocampal neurons using differential detergent extraction and laser scanning microscopy. Axon development correlated with a transition from a MAP2-actin filament interaction to a MAP2-microtubule association and binding of tau to the distal axon. Tau and MAP2 binding specifically increased in the axon and the minor neurites, respectively. No compartment-specific association of MAP5 was observed. Tau binding preceded the accumulation of microtubules at the distal axon which represented a characteristic event during axogenesis. The data provide evidence for a role of MAP2 in regulating microfilament-microtubule interactions during neurite formation and of tau in organizing microtubules at the distal axon.
Kwei
S L
SL
Institute für Neurobiologie, Universität Heidelberg, Germany.
Clement
A
A
Faissner
A
A
Brandt
R
R
D000818:Animals; D001369:Axons / Q000378:metabolism*; D002478:Cells, Cultured; D006624:Hippocampus / Q000166:cytology / Q000378:metabolism*; D008856:Microscopy, Fluorescence; D008869:Microtubule-Associated Proteins / Q000378:metabolism; D009419:Nerve Tissue Proteins / Q000378:metabolism*; D009474:Neurons / Q000378:metabolism*; D011485:Protein Binding; D051381:Rats; D016875:tau Proteins / Q000378:metabolism
D008869:Microtubule-Associated Proteins; D009419:Nerve Tissue Proteins; C085284:microtubule-associated protein 1B; D016875:tau Proteins