title: Coronal leakage of obturated root canals after long-term storage using a polymicrobial marker.
abstract: This in vitro study investigated the effect of long-term storage on the coronal leakage of a microbial marker on teeth root filled with lateral condensation of cold gutta-percha and one of two sealers. Sixty single-rooted teeth were prepared chemomechanically to a size 40 master apical file. The teeth were divided into two groups of 20 teeth each and obturated with gutta-percha using either Apexit or Tubliseal EWT sealer. The teeth were stored for 6 months in artificial saliva and tested for leakage using a marker consisting of S. sanguis and P. intermedia. The teeth were checked for bacterial leakage daily for 90 days. All positive control teeth leaked after 24 h, while the negative control teeth remained uncontaminated throughout the test period. Leakage reached the apex through the experimental teeth at the earliest at 17 days and at latest at 88 days. Fifty percent and 70% of the specimens of the Apexit and Tubliseal EWT groups respectively showed leakage at 90 days. There was no statistically significant difference (p > 0.05) between the two groups.
authors.affiliation: University of Glasgow Dental School, United Kingdom.
authors.forename: P
authors.initials: P
authors.lastname: Chailertvanitkul
authors.forename: W P
authors.initials: WP
authors.lastname: Saunders
authors.forename: D
authors.initials: D
authors.lastname: MacKenzie
mesh_terms: D002126:Calcium Hydroxide / Q000627:therapeutic use; D003763:Dental Leakage / Q000209:etiology / Q000382:microbiology*; D003786:Dental Pulp Cavity / Q000382:microbiology; D006171:Gutta-Percha / Q000627:therapeutic use; D006801:Humans; D066298:In Vitro Techniques; D018713:Prevotella intermedia; D012387:Root Canal Filling Materials / Q000627:therapeutic use; D012389:Root Canal Obturation / Q000295:instrumentation / Q000379:methods; D012464:Saliva, Artificial; D013298:Streptococcus sanguis; D013997:Time Factors; D014021:Tissue Preservation / Q000379:methods; D019228:Tooth Crown / Q000382:microbiology*; D015035:Zinc Oxide-Eugenol Cement
chemical_list: C074418:Apexit; D012387:Root Canal Filling Materials; D012464:Saliva, Artificial; D015035:Zinc Oxide-Eugenol Cement; C048769:tubliseal; D006171:Gutta-Percha; D002126:Calcium Hydroxide