title: Involvement of two isoforms of SNAP-25 in the expression of long-term potentiation in the rat hippocampus.
abstract: Induction of long-term potentiation (LTP) in the hippocampus is associated with changes in expression of a variety of different proteins and is thought to be the mechanism which underlies synaptic plasticity. The 25 kDa synaptosomal-associated protein (SNAP-25) is a presynaptic protein which is involved in neurotransmitter exocytosis at the nerve terminal. Two isoforms of SNAP-25 have so far been identified (a and b) which differ in their distribution and developmental regulation. Using in situ hybridization, we demonstrated that the mRNA levels of the two isoforms of this protein are increased 2 h after the induction of LTP in granule cells of the dentate gyrus following high frequency stimulation of the perforant path in vivo. These observations further demonstrate the involvement of both isoforms of SNAP-25 in functional synaptic plasticity, although their exact roles have yet to be fully determined.
authors.affiliation: Division of Neuroscience and Biomedical Systems, Institute for Biomedical and Life Sciences, University of Glasgow, UK.
authors.forename: L A
authors.initials: LA
authors.lastname: Roberts
authors.forename: B J
authors.initials: BJ
authors.lastname: Morris
authors.forename: C T
authors.initials: CT
authors.lastname: O'Shaughnessy
mesh_terms: D000818:Animals; D019706:Excitatory Postsynaptic Potentials; D006624:Hippocampus / Q000378:metabolism / Q000502:physiology*; D017403:In Situ Hybridization; D007858:Learning / Q000502:physiology; D017774:Long-Term Potentiation; D008297:Male; D008565:Membrane Proteins; D008568:Memory / Q000502:physiology; D009419:Nerve Tissue Proteins / Q000502:physiology*; D018377:Neurotransmitter Agents / Q000378:metabolism; D051381:Rats; D011919:Rats, Inbred Strains; D013569:Synapses / Q000502:physiology; D050825:Synaptosomal-Associated Protein 25
chemical_list: D008565:Membrane Proteins; D009419:Nerve Tissue Proteins; D018377:Neurotransmitter Agents; C494210:Snap25 protein, rat; D050825:Synaptosomal-Associated Protein 25