Comparison of oxidative base damage in mitochondrial and nuclear DNA.
The levels of endogenous pig liver cells mitochondrial DNA oxidative base damage have been investigated using isotope dilution gas chromatography mass spectrometry (GC/MS). Higher levels of five measured bases were found in mtDNA in relation to nuclear DNA. We have also detected large differences in the modified base ratios of mitochondrial versus nuclear DNA. These ratios for the bases with promutagenic properties as 8OHGua and 5OHCyt are much lower than for other bases (5OHHyd, 5OHMeHyd, 5OHMeUra).
Zastawny
T H
TH
Department of Clinical Biochemistry, University School of Medical Sciences, Bydgoszcz, Poland. tomekz@aci.amb.bydgoszcz.pl
Dabrowska
M
M
Jaskolski
T
T
Klimarczyk
M
M
Kulinski
L
L
Koszela
A
A
Szczesniewicz
M
M
Sliwinska
M
M
Witkowski
P
P
Olinski
R
R
D000818:Animals; D002467:Cell Nucleus / Q000235:genetics*; D004249:DNA Damage; D004272:DNA, Mitochondrial / Q000737:chemistry*; D005609:Free Radicals; D008099:Liver / Q000737:chemistry*; D008297:Male; D009711:Nucleotides / Q000737:chemistry*; D010084:Oxidation-Reduction; D013552:Swine
D004272:DNA, Mitochondrial; D005609:Free Radicals; D009711:Nucleotides