[Relationship between INH-resistance and katG mutation in M. tuberculosis].
OBJECTIVE
To investigate the molecular mechanism of INH-resistance and the relationship between INH-resistance and KatG mutations.


METHODS
Use the primers designed from the 5' end of the KatG gene to amplify 282bp fragments from INH-susceptible and -resistant M. tuberculosis isolates. Single strand conformation polymorphism (SSCP) for their PCR products were analysed.


RESULTS
No katG gene sequence deletion was observed, mutations were detected in 17 of 30 INH-resistant strains and no gene perturbations were shown in 16 INH-susceptible isolates.


CONCLUSIONS
The results suggested that mutation in katG gene was one of the most important INH-resistant mechanisms in M. tuberculosis.
Zhang
Z
Z
Beijing Tuberculosis and Thoracic Tumor Institute..
Ma
Y
Y
Cheng
S
S
D000995:Antitubercular Agents / Q000494:pharmacology; D001426:Bacterial Proteins; D004352:Drug Resistance, Microbial / Q000235:genetics; D005798:Genes, Bacterial; D006801:Humans; D007538:Isoniazid / Q000494:pharmacology; D009169:Mycobacterium tuberculosis / Q000235:genetics* / Q000302:isolation & purification; D010544:Peroxidases / Q000235:genetics*; D017354:Point Mutation; D016133:Polymerase Chain Reaction; D018807:Polymorphism, Single-Stranded Conformational; D014397:Tuberculosis, Pulmonary / Q000382:microbiology
D000995:Antitubercular Agents; D001426:Bacterial Proteins; D010544:Peroxidases; C076136:catalase HPI; D007538:Isoniazid