title: [The effect of sampling repetition on gingival crevicular fluid and aspartate aminotransferase activity].
abstract: The purpose of the present investigation was to evaluate the influence on sampling repetition of aspartate aminotransferase (AST) level with 10-minute intervals. Tests based on the composition of gingival crevicular fluid (GCF) for detection of active periodontitis and GCF require the repetition of sampling. Two 30-second samples of GCF were harvested with 10-minute intervals from 123 sites in 10 healthy subjects and 20 periodontitis patients. AST activity of the first samples in periodontitis subjects were approximately 7.8% greater than that of the second samples. The difference were not significant (P > 0.05). But in healthy subjects the difference were significant (P < 0.05). AST activity correlation positively with bleeding index (BI) and probing pocket depth (PD).
authors.affiliation: School of Stomatology, Beijing Medical University.
authors.forename: L
authors.initials: L
authors.lastname: Xu
authors.forename: C
authors.initials: C
authors.lastname: Cao
authors.forename: Y
authors.initials: Y
authors.lastname: Sha
mesh_terms: D000328:Adult; D001219:Aspartate Aminotransferases / Q000378:metabolism*; D005260:Female; D005883:Gingival Crevicular Fluid / Q000201:enzymology*; D006801:Humans; D008297:Male; D008875:Middle Aged; D010512:Periodontal Index; D010518:Periodontitis / Q000201:enzymology* / Q000473:pathology
chemical_list: D001219:Aspartate Aminotransferases