title: Dielectric studies of chain melting and denaturation in native DNA.
abstract: Dielectric methods, based on time domain reflectometry (TDR), have been employed to characterise DNA chain melting and denaturation. A dielectric dispersion has been identified, centred in the low megahertz region, which has been attributed to the fluctuation of counterions along short segments of the DNA chain. Chain melting studies have shown that the magnitude of this dispersion is dependent not only on the relative proportions of single to double stranded DNA but also on the intermolecular interactions between the DNA chains.
authors.affiliation: Institute of Molecular and Biomolecular Electronics, University of Wales, Dean Street, Bangor, Gwynedd, LL57 1UT, UK. rslee@sees.bangor.ac.uk
authors.forename: R S
authors.initials: RS
authors.lastname: Lee
authors.forename: S
authors.initials: S
authors.lastname: Bone
mesh_terms: D000818:Animals; D004247:DNA / Q000737:chemistry*; D004563:Electrochemistry; D005399:Fishes; D066298:In Vitro Techniques; D008297:Male; D008956:Models, Chemical; D009690:Nucleic Acid Conformation; D009691:Nucleic Acid Denaturation; D009994:Osmolar Concentration; D012996:Solutions
chemical_list: D012996:Solutions; D004247:DNA