Cloning and characterization of a novel class I cytokine receptor.
The human gp130 cDNA sequence was used as a query to search an expressed sequence tag database (dbEST) to identify cDNA sequences with similarity to the cytokine class I receptor family. A novel class I cytokine receptor was identified in a human infant brain cDNA library and was named WSX-1. Full-length cDNA sequences for human and murine WSX-1 were isolated and characterized. The WSX-1 cDNA encodes a 636 amino acid transmembrane protein with an extracellular domain of 482 amino acids and a cytoplasmic domain of 96 amino acids. The structure of the WSX-1 protein most closely resembles that of gp130. Northern blot analysis indicates high levels of expression in thymus, spleen, lymph node, and peripheral blood leukocytes, suggesting a role for WSX-1 in modulation of the immune response.
Sprecher
C A
CA
ZymoGenetics Inc., Seattle, Washington 98102, USA.
Grant
F J
FJ
Baumgartner
J W
JW
Presnell
S R
SR
Schrader
S K
SK
Yamagiwa
T
T
Whitmore
T E
TE
O'Hara
P J
PJ
Foster
D F
DF
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D001921:Brain / Q000378:metabolism; D002874:Chromosome Mapping; D003001:Cloning, Molecular; D017124:Conserved Sequence; D017931:DNA Primers / Q000235:genetics; D018076:DNA, Complementary / Q000235:genetics; D006801:Humans; D006822:Hybrid Cells; D007223:Infant; D008024:Ligands; D051379:Mice; D008969:Molecular Sequence Data; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D018121:Receptors, Cytokine / Q000145:classification* / Q000235:genetics* / Q000378:metabolism; D018123:Receptors, Interleukin; D017386:Sequence Homology, Amino Acid; D015398:Signal Transduction; D014018:Tissue Distribution
D017931:DNA Primers; D018076:DNA, Complementary; C112441:IL27RA protein, human; C484747:Il27ra protein, mouse; D008024:Ligands; D012333:RNA, Messenger; D018121:Receptors, Cytokine; D018123:Receptors, Interleukin