RNA aptamers to a protease, subtilisin.
RNA ligands (RNA aptamers) to a protease subtilisin were selected from pools of random RNA using SELEX (Systematic evolution of ligands by exponential enrichment). We selected RNA aptamers using a subtilisin-immobilized Sepharose column. After several rounds of selection, we obtained RNA aptamers that specifically inhibit the subtilisin activity. Characterization of the RNA aptamers will be reported.
Takeno
H
H
Department of Ecological Engineering, Toyohashi University of Technology, Japan.
Tanaka
T
T
Kikuchi
Y
Y
D001665:Binding Sites; D008024:Ligands; D016133:Polymerase Chain Reaction; D011480:Protease Inhibitors / Q000737:chemistry / Q000378:metabolism / Q000494:pharmacology; D012313:RNA / Q000096:biosynthesis* / Q000737:chemistry* / Q000494:pharmacology; D013381:Subtilisins / Q000737:chemistry / Q000378:metabolism*
D008024:Ligands; D011480:Protease Inhibitors; D012313:RNA; D013381:Subtilisins