Deoxycytidine kinase can be also potentiated by the G-protein activator NaF in cells.
Recently, it has been shown, that 2-Chloro-deoxyadenosine (1), a series of analogues, and other DNA synthesis inhibitors, increased the deoxycytidine kinase (dCK) enzyme activity in different cells, without influencing thymidine kinase isoenzymes (TK1, TK2), dCMP-deaminase and thymidylate synthase (TS) activities (2,3). The dCK activity was 2-4 times higher in analogue treated cells, than in controls, which can not be explained by metabolic pool imbalance induced by the drugs. New mRNA and protein synthesis of dCK could not be detected, thus post-translational modification has been suggested for potentiation the activity of the dCK (1). Because secondary modifications of enzymes usually involve the signalling processes in cells, the universal G-protein activator fluorine ions were tested. dCK activity of human lymph node lymphocytes were increased 2-times, if cells were incubated in the presence of NaF for 1-2 hrs in cultures, while TK activity was not changed. The formation of dUTP from dCyd, was also enhanced by NaF, in parallel of dCK potentiation.
Staub
M
M
Semmelweis Medical University, Department of Medical Chemistry, Molecular Biology and Pathobiochemistry, Budapest, Hungary.
Csapó
Z
Z
Spasokukotskaja
T
T
Sasvári-Székely
M
M
D002478:Cells, Cultured; D002648:Child; D002675:Child, Preschool; D003842:Deoxycytidine Kinase / Q000096:biosynthesis / Q000378:metabolism*; D019204:GTP-Binding Proteins / Q000378:metabolism*; D015971:Gene Expression Regulation, Enzymologic / Q000187:drug effects; D006801:Humans; D007527:Isoenzymes / Q000378:metabolism; D008214:Lymphocytes / Q000201:enzymology*; D014066:Palatine Tonsil; D011499:Protein Processing, Post-Translational; D012333:RNA, Messenger / Q000096:biosynthesis; D012969:Sodium Fluoride / Q000494:pharmacology*; D013937:Thymidine Kinase / Q000378:metabolism; D013940:Thymidylate Synthase / Q000378:metabolism; D014158:Transcription, Genetic / Q000187:drug effects
D007527:Isoenzymes; D012333:RNA, Messenger; D012969:Sodium Fluoride; D013940:Thymidylate Synthase; D013937:Thymidine Kinase; D003842:Deoxycytidine Kinase; D019204:GTP-Binding Proteins