Reactivity of workshop monoclonal antibodies on paraformaldehyde-fixed porcine blood mononuclear cells.
One hundred sixty-four monoclonal antibodies (mAbs) of the second international swine CD workshop were tested for their reactivity with porcine blood mononuclear cells before and after fixing the cells with varying concentrations of paraformaldehyde (PFA) (1, 5 and 10 g l-1). A total of 38 (out of 134) positive reacting mAbs were significantly affected in their binding behavior on fixed cells. Modulation was seen as reduction in binding (staining intensity and/or % positive cells, n = 18) or in elevated values (n = 20). Modified mAb binding occurred after fixing cells with 5 to 10 g l-1 PFA.
Schuberth
H J
HJ
Immunology Unit, School of Veterinary Medicine, Hannover, Germany. jschub@immunologie.tiho-hannover.de
Rabe
H U
HU
Leibold
W
W
D000818:Animals; D000911:Antibodies, Monoclonal / Q000097:blood*; D001666:Binding Sites, Antibody; D005260:Female; D005404:Fixatives; D005557:Formaldehyde; D007202:Indicators and Reagents; D007963:Leukocytes, Mononuclear / Q000276:immunology*; D008214:Lymphocytes / Q000276:immunology; D009000:Monocytes / Q000276:immunology; D011108:Polymers; D013552:Swine / Q000276:immunology*
D000911:Antibodies, Monoclonal; D005404:Fixatives; D007202:Indicators and Reagents; D011108:Polymers; D005557:Formaldehyde; C003043:paraform