title: Change in dietary saturated fat intake is correlated with change in mass of large low-density-lipoprotein particles in men.
abstract: We tested whether nutrient intakes estimated from 4-d diet records were associated with plasma lipoprotein subclasses in 103 men who were randomly assigned to a low-fat (24% fat) and a high-fat (46% fat) diet for 6 wk each in a crossover design. Postheparin plasma lipoprotein lipase (LPL) and hepatic lipase (HL) activities were also determined in a subset of 43 men. Changes in intake (ie, high fat minus low fat) of total saturated fatty acids, as well as myristic (14:0) and palmitic (16:0) acids, were positively correlated (P < 0.01) with increases in mass of large LDL particles [measured by analytic ultra-centrifugation as mass of lipoproteins of flotation rate (Sf) 7-12] and with LDL peak particle diameter and flotation rate, but not with changes in LDL-cholesterol concentration. Changes in total saturated fatty acids as well as myristic and palmitic acids were also inversely associated with changes in HL activity (P < 0.05). With the high-fat diet only, variation in dietary total saturated fatty acid intake was inversely correlated (P < 0.01) with concentrations of small, dense LDL of Sf 0-5. This correlation was significant specifically for myristic acid (P < 0.001). Stearic acid (18:0), monounsaturates, and polyunsaturates showed no significant associations with lipoprotein concentrations. These data indicate that a high saturated fat intake (especially 14:0 and 16:0) is associated with increased concentrations of larger, cholesterol-enriched LDL and this occurs in association with decreased HL activity.
authors.affiliation: Children's Hospital Oakland Research Institute, CA, USA.
authors.forename: D M
authors.initials: DM
authors.lastname: Dreon
authors.forename: H A
authors.initials: HA
authors.lastname: Fernstrom
authors.forename: H
authors.initials: H
authors.lastname: Campos
authors.forename: P
authors.initials: P
authors.lastname: Blanche
authors.forename: P T
authors.initials: PT
authors.lastname: Williams
authors.forename: R M
authors.initials: RM
authors.lastname: Krauss
mesh_terms: D000328:Adult; D000368:Aged; D000925:Anticoagulants / Q000494:pharmacology; D018592:Cross-Over Studies; D004041:Dietary Fats / Q000008:administration & dosage* / Q000494:pharmacology; D005227:Fatty Acids / Q000008:administration & dosage* / Q000494:pharmacology; D006493:Heparin / Q000494:pharmacology; D006801:Humans; D008071:Lipoprotein Lipase / Q000187:drug effects / Q000378:metabolism; D008074:Lipoproteins / Q000097:blood / Q000187:drug effects; D008077:Lipoproteins, LDL / Q000097:blood / Q000187:drug effects*; D008099:Liver / Q000187:drug effects / Q000201:enzymology; D008297:Male; D008875:Middle Aged; D012016:Reference Values
chemical_list: D000925:Anticoagulants; D004041:Dietary Fats; D005227:Fatty Acids; D008074:Lipoproteins; D008077:Lipoproteins, LDL; D006493:Heparin; D008071:Lipoprotein Lipase