Novel DNA detection system of flow injection analysis (2). The distinctive properties of a novel system employing PNA (peptide nucleic acid) as a probe for specific DNA detection.
In order to realize immediate detection of a double stranded DNA amplified by Polymerase Chain Reaction (PCR), we applied Peptide Nucleic Acid (PNA) to the probe of DNA detection system using Surface Plasmon Resonance (SPR). We report our success in immediate detection of PCR products solution with high sequence-specificity.
Kai
E
E
Research Center for Advanced Science and Technology, University of Tokyo, Japan.
Sawata
S
S
Ikebukuro
K
K
Iida
T
T
Honda
T
T
Karube
I
I
D001427:Bacterial Toxins / Q000235:genetics*; D004247:DNA / Q000032:analysis*; D015342:DNA Probes; D004269:DNA, Bacterial / Q000032:analysis* / Q000235:genetics; D004926:Escherichia coli / Q000235:genetics*; D010455:Peptides; D016133:Polymerase Chain Reaction / Q000379:methods*; D022641:Shiga Toxin 2
D001427:Bacterial Toxins; D015342:DNA Probes; D004269:DNA, Bacterial; D010455:Peptides; D022641:Shiga Toxin 2; D004247:DNA