title: Signal transduction during Legionella pneumophila entry into human monocytes.
abstract: Legionella pneumophila causes Legionnaires' disease by replication in alveolar macrophages and monocytes. The bacteria are internalized most efficiently by opsonin-dependent, CR3-mediated phagocytosis. This investigation focused on determining the role of actin polymerization and phosphorylation signals in this uptake mechanism. Uptake inhibition assays and confocal microscopic analysis indicated that entry of L. pneumophila activated tyrosine kinase (TK) and protein kinase C (PKC) and induced actin polymerization at the site of bacterial entry. Upon L. pneumophila entry, six major cellular proteins (75, 71, 59, 56, 53, and 52 kDa) were TK phosphorylated in soluble fractions of monocytes, and three of these proteins (52, 53, and 56 kDa) were consistently found in insoluble (i.e., cytoskeletal) fractions of monocytes as well. Tyrosine phosphorylation was suppressed when cells were pretreated with the kinase inhibitor genistein, tyrphostin, or staurosporine. A similar tyrosine-phosphorylated protein pattern was observed with CR3-mediated entry of avirulent L. pneumophila, Escherichia coli, or zymosan into monocytes. This study has shown that PKC and TK signals which activate actin polymerization during the process of phagocytosis are induced upon L. pneumophila entry. In addition, CR3 receptor-mediated phagocytosis into monocytes may involve tyrosine phosphorylation of similar proteins, regardless of the particle being phagocytosed. Therefore, the tyrosine-induced phosphorylation observed during opsonized L. pneumophila entry is not a virulence-associated event.
authors.affiliation: Department of Microbiology and Immunology, University of Louisville, Louisville, Kentucky 40292, USA. pycoxo01@ulkyvm.louisville.edu
authors.forename: P Y
authors.initials: PY
authors.lastname: Coxon
authors.forename: J T
authors.initials: JT
authors.lastname: Summersgill
authors.forename: J A
authors.initials: JA
authors.lastname: Ramirez
authors.forename: R D
authors.initials: RD
authors.lastname: Miller
mesh_terms: D000199:Actins / Q000378:metabolism; D003599:Cytoskeleton; D006801:Humans; D016952:Legionella pneumophila / Q000472:pathogenicity*; D009000:Monocytes / Q000378:metabolism / Q000382:microbiology*; D010587:Phagocytosis; D010766:Phosphorylation; D011493:Protein Kinase C / Q000037:antagonists & inhibitors / Q000378:metabolism; D011505:Protein-Tyrosine Kinases / Q000037:antagonists & inhibitors / Q000378:metabolism; D015398:Signal Transduction
chemical_list: D000199:Actins; D011505:Protein-Tyrosine Kinases; D011493:Protein Kinase C