title: Selective recognition of tandemly repeated DNA sequences by homo- and heterodimers of short peptides.
abstract: The non-covalent and specific protein-protein interaction is critical to the specificity and the cooperativity of the DNA-binding by protein dimers. We have designed and synthesized three sets of peptide dimers with covalent- or noncovalent artificial dimerization modules to elucidate the structural and thermodynamic aspects for the sequence-specific DNA-binding by protein dimers. We have investigated the DNA binding of covalent dimer, noncovalent homodimer and noncovalent heterodimer with specific or nonspecific DNA sequences by gel mobility shift assay. Although the amino acid sequence of DNA-binding region of these peptide dimers are the same, the selectivity and the cooperativity of DNA binding by these peptide dimers were found to be different.
authors.affiliation: Institute for Chemical Research, Kyoto University, Japan.
authors.forename: Y
authors.initials: Y
authors.lastname: Aizawa
authors.forename: T
authors.initials: T
authors.lastname: Morii
authors.forename: Y
authors.initials: Y
authors.lastname: Sugiura
mesh_terms: D000218:Adamantane / Q000737:chemistry; D001483:Base Sequence; D001665:Binding Sites; D003505:Cyclodextrins / Q000737:chemistry; D004247:DNA / Q000737:chemistry* / Q000378:metabolism; D004268:DNA-Binding Proteins / Q000737:chemistry* / Q000378:metabolism*; D019281:Dimerization; D008958:Models, Molecular; D009690:Nucleic Acid Conformation; D009838:Oligodeoxyribonucleotides / Q000737:chemistry; D010455:Peptides / Q000737:chemistry / Q000378:metabolism; D011487:Protein Conformation; D012091:Repetitive Sequences, Nucleic Acid; D047392:beta-Cyclodextrins
chemical_list: D003505:Cyclodextrins; D004268:DNA-Binding Proteins; D009838:Oligodeoxyribonucleotides; D010455:Peptides; D047392:beta-Cyclodextrins; D004247:DNA; C031215:betadex; D000218:Adamantane