Use of muscle fluid as a source of antibodies for serologic detection of Salmonella infection in slaughter pig herds.
Fluid drained from a muscle tissue sample was used as an alternative to serum for the detection of specific anti-Salmonella antibodies in an indirect LPS enzyme-linked immunosorbent assay (ELISA). In the first study, serum and muscle fluid from 3 pigs experimentally infected with Salmonella typhimurium showed parallel dilution-response relationships when ELISA optical density (OD) values were plotted against sample dilution. ELISA results obtained with serum diluted 1:400 corresponded to those from muscle fluid diluted 1:30. In a second study, using the predetermined dilutions of individually paired serum and muscle fluid samples from 103 pigs, a high degree of concordance between the serum ELISA and the muscle fluid ELISA was observed. Limits of agreement between the 2 methods were calculated as -8.9 to 12.3 OD%, which was considered acceptable. The muscle fluid ELISA had specificities of 0.91-1.0 and sensitivities of 0.80-0.89 at various cutoff values as compared with the serum ELISA. Muscle fluid is a useful postmortem alternative to serum when used with an ELISA to detect anti-Salmonella antibodies.
Nielsen
B
B
Danish Veterinary Laboratory, Copenhagen V.
Ekeroth
L
L
Bager
F
F
Lind
P
P
D000003:Abattoirs; D000818:Animals; D000907:Antibodies, Bacterial / Q000032:analysis* / Q000097:blood; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D008460:Meat / Q000382:microbiology*; D018482:Muscle, Skeletal / Q000276:immunology*; D015203:Reproducibility of Results; D012481:Salmonella Infections, Animal / Q000175:diagnosis*; D013552:Swine; D013553:Swine Diseases / Q000175:diagnosis* / Q000382:microbiology
D000907:Antibodies, Bacterial