Effects on brain tumor cell proliferation by an adenovirus vector that bears the interleukin-4 gene.
A recombinant adenovirus vector bearing the IL-4 gene (AD-IL-4) was used to infect rat glioma C6 cells in culture at multiplicity of infections (MOI) from 50 to 1800. C6 cell proliferation was not altered significantly by adenoviral infection. However, IL-4 production increased in a dose-dependent manner. To ascertain effects on in vivo cell proliferation, a subcutaneous tumor model was used. Rat C6 glioma cells alone or C6 mixed with the control virus bearing the LacZ gene (Ad-LacZ) produced tumors that measured an average of approximately 3000 mm3 35 days after implantation. In contrast, C6 cells mixed with Ad-IL-4 produced significant inhibition of tumor growth (P=0.035 compared to C6 tumor; P=0.023 compared to C6+Ad-LacZ tumor. Student's ttest). IL-4 levels in mice serum were measured by ELISA and reached a peak of approximately 700 pg/ml at 14 days. These preliminary results showed that adenovirus-mediated delivery of the IL-4 gene may result in a significant inhibition of rat C6 cell tumor growth. Further studies will be necessary to refine this anti-tumor effect for as a potential therapy for cancer.
Wei
M X
MX
Neurosurgery Service, Massachusetts General Hospital, Harvard Medical School, Boston 02114, USA.
Li
F
F
Ono
Y
Y
Gauldie
J
J
Chiocca
E A
EA
D000256:Adenoviridae / Q000235:genetics; D000818:Animals; D001932:Brain Neoplasms / Q000378:metabolism / Q000473:pathology / Q000628:therapy*; D002455:Cell Division; D005822:Genetic Vectors / Q000008:administration & dosage* / Q000235:genetics; D015847:Interleukin-4 / Q000235:genetics* / Q000378:metabolism; D051379:Mice; D008819:Mice, Nude; D051381:Rats; D014407:Tumor Cells, Cultured
D015847:Interleukin-4