Effect of heparin on hemagglutination by equine arteritis virus.
Heparin inhibited hemagglutination (HA) by equine arteritis virus (EAV) as well as did HA by Aujeszky's disease virus (ADV), but failed to inhibit HA by parainfluenza virus type 3 (PIV-3). The minimal concentration of heparin required to inhibit 8 HA U of EAV was 0.1 U/ml. In addition, most EAV hemagglutinin was retained by heparin acrylic beads, as was ADV hemagglutinin, but was not PIV-3 hemagglutinin. Mouse erythrocytes failed to combine with the HA inhibitory factor of heparin. However, mouse erythrocytes treated with heparinase had greatly reduced agglutinability by EAV. All these findings suggest that a heparin-like molecule on the surface of mouse erythrocytes serves as the virus-cell receptor.
Sano
Y
Y
Department of Veterinary Epizootiology, College of Bioresource Sciences, Nihon University, Kanagawa, Japan.
Inaba
Y
Y
Uwatoko
K
K
Kubota
T
T
Asagoe
T
T
Kanaya
J
J
Pan
I J
IJ
Akashi
H
H
Fukunaga
Y
Y
D000818:Animals; D002417:Cattle; D002460:Cell Line; D007240:Equartevirus / Q000187:drug effects / Q000502:physiology*; D004912:Erythrocytes / Q000187:drug effects / Q000502:physiology / Q000821:virology; D006384:Hemagglutination / Q000187:drug effects*; D006385:Hemagglutination Inhibition Tests; D006386:Hemagglutination Tests; D006389:Hemagglutinins, Viral / Q000502:physiology; D006493:Heparin / Q000494:pharmacology*; D019763:Heparin Lyase / Q000494:pharmacology; D011558:Herpesvirus 1, Suid / Q000502:physiology; D007668:Kidney; D051379:Mice; D011817:Rabbits; D013552:Swine
D006389:Hemagglutinins, Viral; D006493:Heparin; D019763:Heparin Lyase