title: Neural cell adhesion molecule L1 in gliomas: correlation with TGF-beta and p53.
abstract: OBJECTIVE
  To assess immunohistochemically whether the neural cell adhesion molecule L1, which is a member of the immunoglobulin superfamily and has been shown recently to be a stimulating factor for glioma migration, is expressed in glioma tissues, and to investigate factors that can regulate this expression.
  
  
  METHODS
  Twenty seven glioma tissue specimens including 13 glioblastomas, seven anaplastic astrocytomas, and seven astrocytomas were examined. Immunohistochemical analyses of L1, p53, and transforming growth cell factor beta (TGF-beta) were performed on each tumour using both polyclonal and monoclonal antibodies.
  
  
  RESULTS
  Nine (33%) specimens (six glioblastomas and three anaplastic astrocytomas) had L1 positive immunostaining. p53 positive staining was detected in 10 (43%) of 23 glioma specimens (seven glioblastomas and three anaplastic astrocytomas). TGF-beta positive immunostaining was observed in 12 (52%) of the 23 glioma specimens (six glioblastomas, four anaplastic astrocytomas, and two astrocytomas). There was a statistical correlation between both p53 and L1 expression and TGF-beta and L1 expression. No such correlation was found between p53 and TGF-beta expression.
  
  
  CONCLUSIONS
  These results suggest that mutation of the p53 gene or expression of TGF-beta may upregulate the expression of the L1 gene, thus resulting in high grade migration of glioma cells.
authors.affiliation: Department of Neurosurgery, Osaka University Medical School, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Tsuzuki
authors.forename: S
authors.initials: S
authors.lastname: Izumoto
authors.forename: T
authors.initials: T
authors.lastname: Ohnishi
authors.forename: S
authors.initials: S
authors.lastname: Hiraga
authors.forename: N
authors.initials: N
authors.lastname: Arita
authors.forename: T
authors.initials: T
authors.lastname: Hayakawa
mesh_terms: D000951:Antigens, Neoplasm / Q000378:metabolism; D001254:Astrocytoma / Q000378:metabolism; D005909:Glioblastoma / Q000378:metabolism; D005910:Glioma / Q000378:metabolism*; D006801:Humans; D007124:Immunoenzyme Techniques; D039841:Leukocyte L1 Antigen Complex; D009363:Neoplasm Proteins / Q000378:metabolism*; D019006:Neural Cell Adhesion Molecules / Q000378:metabolism*; D016212:Transforming Growth Factor beta / Q000378:metabolism; D016159:Tumor Suppressor Protein p53 / Q000378:metabolism
chemical_list: D000951:Antigens, Neoplasm; D039841:Leukocyte L1 Antigen Complex; D009363:Neoplasm Proteins; D019006:Neural Cell Adhesion Molecules; D016212:Transforming Growth Factor beta; D016159:Tumor Suppressor Protein p53