Racial differences in clinically localized prostate cancers of black and white men.
OBJECTIVE
Tumor grade, deoxyribonucleic acid (DNA) ploidy, proliferation, p53 and bcl-2 expression were examined in clinically localized prostate cancers of black and white American men to learn whether these features showed racial differences.


METHODS
A total of 117 prostate cancers (43 black and 74 white patients) obtained at radical prostatectomy for clinically localized disease were assigned Gleason scores by a single pathologist. Enzymatically dissociated nuclei from archival prostate cancers were examined by DNA flow cytometry using propidium iodide staining and the multicycle program to remove debris and sliced nuclei and to perform cell cycle analysis. For immunostaining after microwave antigen retrieval we used a DO-1/DO-7 monoclonal antibody cocktail for p53 and the clone 124 antibody for bcl-2.


RESULTS
Significantly more black than white men had Gleason score 7 tumors. The DNA ploidy distribution of Gleason 6 or less tumors was similar for both races. As anticipated, the ploidy distribution of higher grade prostate cancer in white men was more abnormal but, unexpectedly, this was not found for higher grade prostate cancer in black men. No significant racial differences were found in S phase fractions, p53 or bcl-2 immunopositivity. However, for prostate cancer in black men there was a significant association between bcl-2 immunopositivity and higher S-phase fractions.


CONCLUSIONS
The aggressive prostate cancers of black men may be characterized by the 2 features of high proliferation and a block to programmed cell death.
deVere White
R W
RW
Department of Urology, University of California, Davis, USA.
Deitch
A D
AD
Jackson
A G
AG
Gandour-Edwards
R
R
Marshalleck
J
J
Soares
S E
SE
Toscano
S N
SN
Lunetta
J M
JM
Stewart
S L
SL
D017209:Apoptosis; D014408:Biomarkers, Tumor; D044383:Black People / Q000235:genetics*; D002455:Cell Division; D004273:DNA, Neoplasm / Q000235:genetics; D005434:Flow Cytometry; D019254:Genes, bcl-2; D016158:Genes, p53; D006801:Humans; D007150:Immunohistochemistry; D008297:Male; D011003:Ploidies; D011379:Prognosis; D011471:Prostatic Neoplasms / Q000208:ethnology / Q000235:genetics* / Q000473:pathology; D044465:White People / Q000235:genetics*
D014408:Biomarkers, Tumor; D004273:DNA, Neoplasm