title: Rescue of the mutant phenotype by reexpression of full-length vinculin in null F9 cells; effects on cell locomotion by domain deleted vinculin.
abstract: Vinculin plays a role in signaling between integrins and the actin cytoskeleton. We reported earlier that F9-derived cells lacking vinculin are less spread, less adhesive, and move two times faster than wild-type F9 cells. Expression of intact vinculin in null cells restored all wild-type characteristics. In contrast, expression of the head (90 kDa) fragment exaggerated mutant characteristics, especially locomotion, which was double that of vinculin null cells. Expression of the tail domain also had a marked effect on locomotion in the opposite direction, reducing it to very low levels. The expression of the head plus tail domains together (no covalent attachment) effected a partial rescue towards wild-type phenotype, thus indicating that reexpressed polypeptides may be in their correct location and are interacting normally. Therefore, we conclude that: (1) the head domain is part of the locomotory force of the cell, modulated by the tail, and driven by the integrin/matrix connection; (2) intact vinculin is required for normal regulation of cell behavior, suggesting that vinculin head-tail interactions control cell adhesion, spreading, lamellipodia formation and locomotion.
authors.affiliation: The Burnham Institute, La Jolla Cancer Research Center, La Jolla, CA92037, USA. eadamson@burnham
authors.forename: W
authors.initials: W
authors.lastname: Xu
authors.forename: J L
authors.initials: JL
authors.lastname: Coll
authors.forename: E D
authors.initials: ED
authors.lastname: Adamson
mesh_terms: D002460:Cell Line; D002465:Cell Movement / Q000502:physiology*; D006801:Humans; D018613:Microscopy, Confocal; D009154:Mutation; D014162:Transfection; D016596:Vinculin / Q000502:physiology*
chemical_list: D016596:Vinculin