title: A CD4+ proliferation of large granular lymphocytes expresses the protease activated receptor-1.
abstract: The platelet-type thrombin receptor was the first member to be identified in a family of protease activated receptors (PARs) and has been designated PAR-1. We recently reported that the large granular lymphocytes (LGLs) in patients with proliferations of CD8+ cells co-expressed PAR-1 and the expression of PAR-1 correlated with the expression of CD57. Here we show, by three-colour immunofluorescence, that the LGLs from a patient with a rare CD4+ CD57+ monoclonal expansion also expressed PAR-1. Northern blot analysis confirmed the presence of high levels of mRNA for PAR-1 in these LGLs.
authors.affiliation: Department of Haematology, The Royal London Hospital, Whitechapel.
authors.forename: M G
authors.initials: MG
authors.lastname: Macey
authors.forename: L
authors.initials: L
authors.lastname: Hou
authors.forename: T
authors.initials: T
authors.lastname: Milne
authors.forename: V
authors.initials: V
authors.lastname: Parameswaren
authors.forename: D
authors.initials: D
authors.lastname: Howe
authors.forename: J D
authors.initials: JD
authors.lastname: Cavenagh
authors.forename: G L
authors.initials: GL
authors.lastname: Howells
authors.forename: A C
authors.initials: AC
authors.lastname: Newland
mesh_terms: D000368:Aged; D015152:Blotting, Northern; D015704:CD4 Antigens; D002455:Cell Division; D005434:Flow Cytometry; D015329:Gene Rearrangement, T-Lymphocyte; D006098:Granulocytes / Q000378:metabolism / Q000473:pathology*; D006801:Humans; D008218:Lymphocytosis / Q000378:metabolism / Q000473:pathology*; D008297:Male; D044463:Receptor, PAR-1; D018179:Receptors, Thrombin / Q000378:metabolism*
chemical_list: D015704:CD4 Antigens; D044463:Receptor, PAR-1; D018179:Receptors, Thrombin