[A study on glycosylated high density lipoprotein and its binding to receptors in human lung fibroblasts in diabetic patients].
In order to know the glycosylation of high density lipoprotein (HDL) and its specific binding to human lung fibroblasts (HLF) receptors in diabetic patients, the glycosyflation of HDL in diabetic group I (HbAlc < 7.6%) and II (HNbAlc > 9.6%) and a control group were measured with fluorimetry. The hydrazides in carbonyl radical of HDL in the three groups were conjugated with horadish peroxidase (HRP). The specific binding of HDL-HRP to HLF receptors was measured with enzyme linked immunoreceptor assay. The results showed that the glycosylated amount of HDl in group I, II and the control group was 39.26 +/- 8.10, 72.96 +/- 6.40 and 20.40 +/- 1.10 glycogroups/HDL respectively. The surface specific binding of HDL-HRP to HLF receptors in medium with high cholesterol was significantly greater than that in medium without cholesterol (P < 0.01). The surface specific binding of HDL-HRP to HLF receptors in group I and II was significantly lower than that in the control group (P < 0.01) and that in group II was significantly lower than in group I (P < 0.01). The results indicate glycosylated HDL in diabetic patients is increased but its specific binding to HLF receptors is decreased as compared with that in control subjects.
Lei
T
T
Department of Endocrinology, Ganquan Hospital, Shanghai Railway University.
Gu
J
J
Chen
H
H
D000368:Aged; D003924:Diabetes Mellitus, Type 2 / Q000378:metabolism*; D005347:Fibroblasts / Q000378:metabolism; D006031:Glycosylation; D006801:Humans; D008075:Lipoproteins, HDL / Q000378:metabolism*; D008168:Lung / Q000166:cytology / Q000378:metabolism*; D008875:Middle Aged; D011956:Receptors, Cell Surface / Q000378:metabolism*
D008075:Lipoproteins, HDL; D011956:Receptors, Cell Surface