Morphological consequences of altered calcium-dependent transmembrane signaling on the development of cultured cerebellar Purkinje neurons.
Morphometric analyses of cultured rat Purkinje neurons, visualized with anti-calbindin, demonstrated that elevated KCl (10 mM) significantly increased dendritic outgrowth and branching. The response was blocked by NiCl2 (50 microM; R-type Ca2+ channel antagonist). Cells grown in low external Ca2+ (100 nM) showed no loss of responsiveness to elevated potassium. However, thapsigargin (1 microM; Ca(2+)-ATPase blocker) inhibited dendrite outgrowth, suggesting that intracellular calcium stores may be important in governing development.
Reitstetter
R
R
Dept. of Physiology, University of Arizona, Tucson 85724-5051, USA.
Yool
A J
AJ
D000818:Animals; D002118:Calcium / Q000494:pharmacology / Q000502:physiology*; D016922:Cellular Senescence / Q000502:physiology; D003712:Dendrites / Q000187:drug effects / Q000502:physiology; D011188:Potassium / Q000494:pharmacology; D011689:Purkinje Cells / Q000166:cytology* / Q000187:drug effects / Q000502:physiology*; D051381:Rats; D015398:Signal Transduction / Q000502:physiology*; D019284:Thapsigargin / Q000494:pharmacology
D019284:Thapsigargin; D011188:Potassium; D002118:Calcium