[The measuring of blood plasma antioxidant activity by the hemoglobin-hydrogen peroxide-luminol system].
The method of valuation of the blood plasma antioxidant activity (AOA) by the hemoglobin-hydrogen peroxide-luminol chemiluminescence system has been proposed. The method is based on the measuring the induction time of chemiluminescence that is directly proportional to the added volume of plasma or concentration of standard antioxidant. The ascorbate was taken as a standard. Blood plasma AOA has been expressed through the concentration of equivalent ascorbate solution (ascorbate equivalent). The influence of hemolysis and storage conditions of plasma on its detected AOA was investigated. The change of human blood plasma AOA after single administration of 2 g the ascorbate and its dynamics in patients with acute pancreatitis was studied.
Teselkin
Iu O
IuO
Department of Biophysics, Russian State Medical University, Moscow, Russia.
Babenkova
I V
IV
Liubitskiĭ
O B
OB
Klebanov
G I
GI
Vladimirov
Iu A
IuA
D000208:Acute Disease; D000328:Adult; D000975:Antioxidants / Q000032:analysis*; D001205:Ascorbic Acid / Q000008:administration & dosage; D005609:Free Radicals; D006454:Hemoglobins / Q000032:analysis; D006801:Humans; D006861:Hydrogen Peroxide / Q000032:analysis; D008163:Luminescent Measurements; D008165:Luminol; D008297:Male; D008875:Middle Aged; D010084:Oxidation-Reduction; D010195:Pancreatitis / Q000097:blood*
D000975:Antioxidants; D005609:Free Radicals; D006454:Hemoglobins; D008165:Luminol; D006861:Hydrogen Peroxide; D001205:Ascorbic Acid
Opredelenie antioksidantnoĭ aktivnosti plazmy krovi c pomoshch'iu sistemy gemoglobin-peroksid vodoroda-liuminol.