Interaction of Rho1p target Bni1p with F-actin-binding elongation factor 1alpha: implication in Rho1p-regulated reorganization of the actin cytoskeleton in Saccharomyces cerevisiae.
The RHO1 gene encodes a homolog of mammalian RhoA small G protein in the yeast Saccharomyces cerevisiae. We have shown that Bni1p is one of the downstream targets of Rho1p and regulates reorganization of the actin cytoskeleton through the interaction with profilin, an actin monomer-binding protein. A Bni1p-binding protein was affinity purified from the yeast cytosol fraction and was identified to be Tef1p/Tef2p, translation elongation factor 1alpha (EF1alpha). EF1alpha is an essential component of the protein synthetic machinery and also possesses the actin filament (F-actin)-binding and -bundling activities. EF1alpha bound to the 186 amino acids region of Bni1p, located between the FH1 domain, the proline-rich profilin-binding domain, and the FH2 domain, of which function is not known. The binding of Bni1p to EF1alpha inhibited its F-actin-binding and -bundling activities. The BNI1 gene deleted in the EF1alpha-binding region did not suppress the bni1 bnr1 mutation in which the actin organization was impaired. These results suggest that the Rho1p-Bni1p system regulates reorganization of the actin cytoskeleton through the interaction with both EF1alpha and profilin.
Umikawa
M
M
Department of Molecular Biology and Biochemistry, Osaka University Medical School, Suita, Japan.
Tanaka
K
K
Kamei
T
T
Shimizu
K
K
Imamura
H
H
Sasaki
T
T
Takai
Y
Y
D000199:Actins / Q000378:metabolism*; D001665:Binding Sites; D002352:Carrier Proteins / Q000302:isolation & purification; D003599:Cytoskeleton / Q000378:metabolism*; D005656:Fungal Proteins / Q000378:metabolism; D019204:GTP-Binding Proteins / Q000502:physiology*; D008565:Membrane Proteins / Q000502:physiology*; D008840:Microfilament Proteins; D020648:Peptide Elongation Factor 1; D010445:Peptide Elongation Factors / Q000378:metabolism*; D012441:Saccharomyces cerevisiae / Q000378:metabolism*; D029701:Saccharomyces cerevisiae Proteins; D020743:rhoB GTP-Binding Protein
D000199:Actins; C105191:Bni1 protein, S cerevisiae; D002352:Carrier Proteins; D005656:Fungal Proteins; D008565:Membrane Proteins; D008840:Microfilament Proteins; D020648:Peptide Elongation Factor 1; D010445:Peptide Elongation Factors; D029701:Saccharomyces cerevisiae Proteins; D019204:GTP-Binding Proteins; D020743:rhoB GTP-Binding Protein