Affinity capillary electrophoresis using DNA conjugates.
An oligonucleotide ((dT)12)-immobilized capillary was prepared for the affinity capillary electrophoresis of DNA. Oligonucleotides ((dA)6 and (dT)6) which had the same chain length but had different base composition were separated in high resolution in the presence of Mg2+ ion. Whereas (dA)6 eluted slightly earlier than (dT)6 in a conventional polyacrylamide-coated capillary, the order of their mobilities was reversed in the (dT)12-modified system, suggesting the complementary hydrogen bondings between (dA)6 and the immobilized (dT)12.
Ozaki
Y
Y
Department of Chemical Science and Technology, Faculty of Engineering, Kyushu University, Fukuoka, Japan.
Ihara
T
T
Katayama
Y
Y
Maeda
M
M
D001482:Base Composition; D001483:Base Sequence; D004247:DNA / Q000737:chemistry* / Q000302:isolation & purification*; D019075:Electrophoresis, Capillary / Q000379:methods*; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods; D006860:Hydrogen Bonding; D015636:Magnesium Chloride; D009838:Oligodeoxyribonucleotides / Q000737:chemistry / Q000302:isolation & purification*
D009838:Oligodeoxyribonucleotides; C027903:oligo (dT); C030570:oligodeoxyadenylic acid; D015636:Magnesium Chloride; D004247:DNA