title: Sequence analysis of MHC class I alpha 2 domain exon variants in one diploid and two haploid Atlantic salmon pedigrees.
abstract: Genetic diversity in the second domain exon of Atlantic salmon (Salmo salar) major histocompatibility complex (Mhc) class I was investigated in two dams and nine of their haploid offspring by means of polymerase chain reaction (PCR) and DNA sequence analysis. A similar study was also performed on nine diploid offspring from one of these dams. The complex segregation patterns and sequence similarities between variants make definitive allele, haplotype and locus assignments difficult. There are, however, indications of six Mhc-Sasa class I loci and a fairly well-defined haplotype of four variants. One non-polymorphic variant present in most specimens could be a salmon analogue to the human non-classical loci.
authors.affiliation: Department of Morphology, Genetics and Aquatic Biology, Norwegian College of Veterinary Medicine, Oslo, Norway.
authors.forename: U
authors.initials: U
authors.lastname: Grimholt
authors.forename: I
authors.initials: I
authors.lastname: Olsaker
authors.forename: F
authors.initials: F
authors.lastname: Lingaas
authors.forename: O
authors.initials: O
authors.lastname: Lie
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D004171:Diploidy; D005260:Female; D005805:Genes, MHC Class I; D014644:Genetic Variation; D015234:HLA-A Antigens / Q000235:genetics; D015235:HLA-B Antigens / Q000235:genetics; D006238:Haploidy; D006801:Humans; D008285:Major Histocompatibility Complex; D008297:Male; D008969:Molecular Sequence Data; D010375:Pedigree; D016133:Polymerase Chain Reaction; D012474:Salmon / Q000235:genetics* / Q000276:immunology; D016415:Sequence Alignment; D012689:Sequence Homology, Nucleic Acid
chemical_list: D015234:HLA-A Antigens; D015235:HLA-B Antigens