title: Influence of plant genotype on the selection of nodulating Sinorhizobium meliloti strains by Medicago sativa.
abstract: We analysed the genetic diversity of 270 Sinorhizobium meliloti strains isolated from nodules of three different Medicago sativa varieties, planted in three different Italian soils, combining the Analysis of Molecular Variance (AMOVA) with the Random Amplified Polymorphic DNA (RAPD) technique to estimate variance among RAPD patterns with the aim to draw an objective description of the population genetic structure. Results indicated that a general intraspecific genetic diversity was globally distributed among all the population, however a very high level of diversity was found among strains nodulating different Medicago sativa varieties. Moreover the distribution of the RAPD haplotypes among the plant varieties also showed to be non-random. The overall data indicated that the plant genotype is a major factor in shaping the genetic structure of this natural Rhizobium population.
authors.affiliation: Dipartimento di Ortoflorofrutticoltura, Università degli Studi di Firenze, Florence, Italy.
authors.forename: D
authors.initials: D
authors.lastname: Paffetti
authors.forename: F
authors.initials: F
authors.lastname: Daguin
authors.forename: S
authors.initials: S
authors.lastname: Fancelli
authors.forename: S
authors.initials: S
authors.lastname: Gnocchi
authors.forename: F
authors.initials: F
authors.lastname: Lippi
authors.forename: C
authors.initials: C
authors.lastname: Scotti
authors.forename: M
authors.initials: M
authors.lastname: Bazzicalupo
mesh_terms: D000704:Analysis of Variance; D004269:DNA, Bacterial / Q000032:analysis; D014644:Genetic Variation; D005838:Genotype; D006239:Haplotypes; D007558:Italy; D000455:Medicago sativa / Q000235:genetics / Q000382:microbiology*; D010802:Phylogeny; D019105:Random Amplified Polymorphic DNA Technique; D012230:Rhizobiaceae / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification / Q000502:physiology; D012988:Soil Microbiology
chemical_list: D004269:DNA, Bacterial