title: Use of hemoglobin as an iron source by Bacillus cereus.
abstract: The hemoglobin binding activity of Bacillus cereus cells was measured with fluoresceinisothiocyanate (FITC)-conjugated hemoglobin using flow cytometry. Growth of B. cereus was markedly inhibited by the addition of apo-transferrin. B. cereus could not use transferrin-bound iron as an iron source in serum. The growth inhibition was reversed by the addition of a FeCl3 solution, erythrocytes or hemoglobin. B. cereus released hemolysin; these findings suggested that the hemoglobin released from erythrocytes by B. cereus hemolysin binds to B. cereus and is thus used as an iron source.
authors.affiliation: Department of Microbiology, Tohoku College of Pharmacy, Sendai, Japan.
authors.forename: N
authors.initials: N
authors.lastname: Sato
authors.forename: H
authors.initials: H
authors.lastname: Kurotaki
authors.forename: T
authors.initials: T
authors.lastname: Watanabe
authors.forename: T
authors.initials: T
authors.lastname: Mikami
authors.forename: T
authors.initials: T
authors.lastname: Matsumoto
mesh_terms: D001059:Apoproteins / Q000494:pharmacology; D001409:Bacillus cereus / Q000235:genetics* / Q000378:metabolism; D003470:Culture Media; D004912:Erythrocytes / Q000378:metabolism; D006454:Hemoglobins / Q000378:metabolism*; D006461:Hemolysis / Q000187:drug effects; D006801:Humans; D066298:In Vitro Techniques; D007501:Iron / Q000378:metabolism*; D014168:Transferrin / Q000494:pharmacology
chemical_list: D001059:Apoproteins; D003470:Culture Media; D006454:Hemoglobins; D014168:Transferrin; C028284:apotransferrin; D007501:Iron