[An experimental study of tumor necrosis factor (TNF) and interleukin-1 (IL-1) in aqueous humor after intraocular lens implantation].
OBJECTIVE
The tumor necrosis factor (TNF) and interleukin-1 (IL-1) levels in aqueous humor after intraocular lens (IOL) implantation were studied in rabbit eyes and their effects on post-operative anterior ocular inflammation were discussed.


METHODS
27 adult pigmented rabbits were divided into three groups: (1) Lens extraction and intraocular lens (IOL) implantation in the capsular bag. (2) Simple extracapsular lens extraction, and (3) normal control group without surgical intervention. Aqueous humor samples were aspirated on days 1, 3, 7 and 14 postoperatively. A modified double-antibody indirect sandwich ELISA was used to detect for the presence of TNF. MTT colorimetry was used to detect for the presence of IL-1. The data were analysed statistically.


RESULTS
TNF and IL-1 levels in the aqueous humor were significantly higher (P < 0.05) at 1, 3, 7, and 14 days postoperatively in IOL implanted group than that in the control group, and TNF levels in aqueous humor at 3, 7 and 14 days postoperatively and IL-1 levels at 1 and 3 days postoperatively were significantly higher (P < 0.05) in IOL implanted group than that in simple extracapsular lens extraction group (P < 0.05). In the postoperative 7-14 days, the TNF and in the 3 to 14 days the IL-1 in the aqueous humor reached their maximum levels in IOL implantation group.


CONCLUSIONS
It is reasonable to consider that the increases of TNF and IL-1 levels may play critical roles in the anterior ocular inflammation response after IOL implantation.
Zhou
Z
Z
Department of Ophthalmology, No. 304 Hospital, Beijing.
He
S
S
Cheng
X
X
D000818:Animals; D001082:Aqueous Humor / Q000378:metabolism*; D002387:Cataract Extraction; D007375:Interleukin-1 / Q000378:metabolism*; D019654:Lens Implantation, Intraocular; D011184:Postoperative Period; D011817:Rabbits; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism*
D007375:Interleukin-1; D014409:Tumor Necrosis Factor-alpha