title: Chemical speciation of arsenic in serum of uraemic patients.
abstract: Chemical speciation of arsenic was carried out in serum of a total of 51 uraemic patients: 19 non-dialysis (ND), 18 haemodialysis (HD) and 14 continuous ambulatory peritoneal dialysis (CAPD) patients. The low molecular mass As species were separated by ion-exchange liquid chromatography and measured on-line by hydride generation atomic absorption spectrometry (HGAAS). The high molecular mass As species were separated by fast protein liquid chromatography, either size-exclusion, ion-exchange or affinity chromatography, and the fractions were digested and measured off-line with HGAAS. The mean total As concentrations in the serum of the three groups of the uraemic patients were significantly higher than the reference value (6.47 +/- 4.28, 5.12 +/- 5.58 and 4.67 +/- 5.41 micrograms l-1 for HD, ND and CAPD patients, respectively, versus the reference value of 0.96 +/- 1.52 micrograms l-1. The major As species in serum of the patients were dimethylarsinic acid (DMA) and arsenobetaine. The HD patients showed a significantly higher mean DMA level than ND and CAPD patients. No selective removal of different As species in serum of HD patients was observed after 4 h of haemodialysis. The inorganic As species in serum were bound to proteins, mainly transferrin (about 5-6% of total As in serum). This binding may play an important role in arsenic detoxification.
authors.affiliation: Laboratory for Analytical Chemistry, University of Ghent, Belgium.
authors.forename: X
authors.initials: X
authors.lastname: Zhang
authors.forename: R
authors.initials: R
authors.lastname: Cornelis
authors.forename: L
authors.initials: L
authors.lastname: Mees
authors.forename: R
authors.initials: R
authors.lastname: Vanholder
authors.forename: N
authors.initials: N
authors.lastname: Lameire
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D001151:Arsenic / Q000097:blood* / Q000378:metabolism; D001152:Arsenicals / Q000097:blood; D001798:Blood Proteins / Q000378:metabolism; D002101:Cacodylic Acid / Q000097:blood; D002852:Chromatography, Ion Exchange; D003404:Creatinine / Q000097:blood; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D008970:Molecular Weight; D010531:Peritoneal Dialysis, Continuous Ambulatory; D006435:Renal Dialysis; D051437:Renal Insufficiency / Q000097:blood / Q000378:metabolism; D013054:Spectrophotometry, Atomic; D014511:Uremia / Q000097:blood* / Q000378:metabolism / Q000628:therapy
chemical_list: D001152:Arsenicals; D001798:Blood Proteins; D002101:Cacodylic Acid; D003404:Creatinine; D001151:Arsenic; C038992:arsenobetaine