title: [Establishment and characterization of primitive neuroectodermal tumor cell line (UTO-EW1)].
abstract: New human primitive neuroectodermal tumor cell line, UTO-EW1 was established from the humeral bone tumor of a 25-year-old male patient. UTO-EW1 cells were found to be small round shaped and grew floating in the medium, or found to form monolayers in a polygonal shaped cells. The modal clomosomal number of the cells fell in a hypo-triploid range with several clonal clomosomal aberrations, including del(22) (q12). Neuron-specific enolase was positive by stained immunohistochemically. This cell line may be useful for basic studies and treatment of PNET.
authors.affiliation: Department of Orthopaedic Surgery, University of Tsukuba, Ibaraki-Ken, Japan.
authors.forename: M
authors.initials: M
authors.lastname: Onobori
authors.forename: K
authors.initials: K
authors.lastname: Hayashi
mesh_terms: D000328:Adult; D000818:Animals; D002460:Cell Line; D002869:Chromosome Aberrations; D006801:Humans; D007150:Immunohistochemistry; D007621:Karyotyping; D008297:Male; D051379:Mice; D016513:Mice, SCID; D018242:Neuroectodermal Tumors, Primitive / Q000235:genetics / Q000473:pathology*; D014407:Tumor Cells, Cultured