LcrG is required for efficient translocation of Yersinia Yop effector proteins into eukaryotic cells.
Extracellular Yersinia disables the immune system of its host by injecting effector Yop proteins into host cells. We show that a Yersinia enterocolitica nonpolar lcrG mutant is severely impaired in the translocation of YopE, YopH, YopM, YpkA/YopO, and YopP into eukaryotic cells. LcrG is thus required for efficient internalization of all the known Yop effectors.
Sarker
M R
MR
Microbial Pathogenesis Unit, Christian de Duve Institute of Cellular Pathology, and Faculté de Médecine, Université Catholique de Louvain, B-1200 Brussels, Belgium.
Sory
M P
MP
Boyd
A P
AP
Iriarte
M
M
Cornelis
G R
GR
D001425:Bacterial Outer Membrane Proteins / Q000378:metabolism*; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism*; D001427:Bacterial Toxins / Q000378:metabolism; D001692:Biological Transport; D003603:Cytotoxins / Q000378:metabolism; D005057:Eukaryotic Cells / Q000382:microbiology; D006367:HeLa Cells / Q000382:microbiology; D006801:Humans; D008264:Macrophages / Q000382:microbiology; D009154:Mutation; D052899:Pore Forming Cytotoxic Proteins; D017027:Protein Tyrosine Phosphatases / Q000378:metabolism; D015008:Yersinia enterocolitica / Q000235:genetics / Q000378:metabolism / Q000472:pathogenicity*
D001425:Bacterial Outer Membrane Proteins; D001426:Bacterial Proteins; D001427:Bacterial Toxins; D003603:Cytotoxins; C088009:LcrG protein, Yersinia; D052899:Pore Forming Cytotoxic Proteins; C063239:yopE protein, Yersinia; D017027:Protein Tyrosine Phosphatases; C067571:yopH protein, Yersinia