Stretching exercises--flexibility in dihydrofolate reductase catalysis.
As an enzyme, dihydrofolate reductase performs two tasks: transformation of its substrate dihydrofolate or folate to tetrahydrofolate, using NADPH as a cofactor, and regeneration of the enzyme for a subsequent round of catalysis. Studies discussed in this review highlight the role of conformational flexibility in both of these enzymatic functions.
Miller
G P
GP
Department of Chemistry, Pennsylvania State University, University Park 16802, USA.
Benkovic
S J
SJ
D002384:Catalysis; D018360:Crystallography, X-Ray; D008024:Ligands; D011487:Protein Conformation; D013762:Tetrahydrofolate Dehydrogenase / Q000737:chemistry / Q000378:metabolism*
D008024:Ligands; D013762:Tetrahydrofolate Dehydrogenase