Inhibitory effect of roxithromycin on adhesion of Klebsiella pneumoniae strains 3051, CF504 and LM21.
The effect of subinhibitory concentrations of roxithromycin on the adhesion of three strains of Klebsiella pneumoniae to Int-407 cells was studied. Adherence was markedly inhibited and the effect was increased when roxithromycin was added to the cell culture medium rather than to the bacterial growth medium. Several assays were performed in order to understand the mechanism by which roxithromycin exerted this inhibitory effect. The greatest effect was obtained when roxithromycin was concentrated in the extracellular compartment; when roxithromycin was concentrated in the intracellular compartment, the inhibitory effect was reduced. The analysis of adhesion factors of bacteria showed that exposure to roxithromycin did not alter their apparent structure or quantity. Roxithromycin appears to interfere in the interaction between bacteria and eukaryotic receptors.
Favre-Bonté
S
S
Laboratoire de Bactériologie, Faculté de Pharmacie, Université d'Auvergne, Clermont-Ferrand, France.
Forestier
C
C
Joly
B
B
D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D001422:Bacterial Adhesion / Q000187:drug effects*; D001425:Bacterial Outer Membrane Proteins / Q000378:metabolism; D002478:Cells, Cultured; D003470:Culture Media; D006801:Humans; D007082:Ileum / Q000382:microbiology; D007583:Jejunum / Q000382:microbiology; D007711:Klebsiella pneumoniae / Q000187:drug effects* / Q000378:metabolism; D008826:Microbial Sensitivity Tests; D011135:Polysaccharides, Bacterial / Q000378:metabolism; D015575:Roxithromycin / Q000494:pharmacology*
D000900:Anti-Bacterial Agents; D001425:Bacterial Outer Membrane Proteins; C072092:CF29K adhesive factor; D003470:Culture Media; D011135:Polysaccharides, Bacterial; D015575:Roxithromycin