title: Involvement of thyroid hormone and its alpha receptor in avian neurulation.
abstract: We have analyzed the expression pattern of c-erb A alpha and c-erb A beta which encode the thyroid hormone receptors (T3R alpha and T3R beta) during early chicken embryogenesis. Only c-erb A alpha expression was detected by RT-PCR and whole-mount in situ hybridization. c-erb A alpha transcripts were found to be already present at low level in embryos before egg incubation. During neurulation a marked increase was observed in neurectoderm. A reporter cell line was then constructed and used to demonstrate the release of significant amount of thyroid hormone (T3) from egg yolk by area opaca cells before gastrulation. During gastrulation T3 was found to be enriched in the primitive streak and Hensen's node. Introduction of excess T3 frequently resulted in abnormal development of anterior structures, mainly neural tube defects and anencephalia. These observations suggest that T3R alpha, like the closely related retinoic acid receptors, fulfills functions which are important for embryonic development well before the onset of thyroid gland function.
authors.affiliation: Laboratoire de Biologie Moléculaire et Cellulaire, l'Ecole Normale Supérieure de Lyon CNRS UMR49-INRA LA913, Allée d'Italie, Lyon Cedex 07, France. Frederic.Flamant@ens-lyon.fr
authors.forename: F
authors.initials: F
authors.lastname: Flamant
authors.forename: J
authors.initials: J
authors.lastname: Samarut
mesh_terms: D000818:Animals; D001755:Blastocyst / Q000378:metabolism; D002642:Chick Embryo; D018920:Coculture Techniques; D046508:Culture Techniques; D004475:Ectoderm / Q000502:physiology; D005775:Gastrula; D017403:In Situ Hybridization; D009024:Morphogenesis; D009420:Nervous System / Q000196:embryology*; D011988:Receptors, Thyroid Hormone / Q000502:physiology*; D014212:Tretinoin / Q000502:physiology; D014284:Triiodothyronine / Q000502:physiology*
chemical_list: D011988:Receptors, Thyroid Hormone; D014284:Triiodothyronine; D014212:Tretinoin