title: Effect of herbimycin A on hsp30 and hsp70 heat shock protein gene expression in Xenopus cultured cells.
abstract: We have examined the effect of herbimycin A, a benzoquinoid ansamycin antibiotic, on the pattern of gene expression in amphibians. Exposure of Xenopus laevis A6 kidney epithelial cells to 1 microgram/mL herbimycin A induced the synthesis of the heat shock proteins hsp30 and hsp70 as well as 33- and 45-kDa proteins. Enhanced synthesis of a 34-kDa protein appears to be specific to herbimycin A because its synthesis did not increase after heat shock (35 degrees C). In addition, the synthesis of hsp30 and hsp70 induced by herbimycin A was accompanied by an increase in their mRNAs. Herbimycin A induced a transient accumulation of hsp30 and hsp70 mRNA, which peaked between 4 and 6 h. Finally, concurrent treatment of cells with 0.5 microgram/mL herbimycin A and a mild heat shock of 27 degrees C yielded a synergistic accumulation of hsp30 and hsp70 mRNA. These studies demonstrate that herbimycin A can induce the expression of a set of stress proteins in amphibians and that concurrent treatment with herbimycin A and mild heat shock has a synergistic effect on the accumulation of hsp30 and hsp70 mRNA.
authors.affiliation: Department of Biology, University of Waterloo, ON, Canada.
authors.forename: D
authors.initials: D
authors.lastname: Briant
authors.forename: N
authors.initials: N
authors.lastname: Ohan
authors.forename: J J
authors.initials: JJ
authors.lastname: Heikkila
mesh_terms: D000818:Animals; D016227:Benzoquinones; D002460:Cell Line; D004847:Epithelial Cells; D005786:Gene Expression Regulation / Q000187:drug effects*; D050890:HSP30 Heat-Shock Proteins; D018840:HSP70 Heat-Shock Proteins / Q000096:biosynthesis / Q000187:drug effects* / Q000235:genetics*; D006360:Heat-Shock Proteins / Q000096:biosynthesis / Q000187:drug effects* / Q000235:genetics*; D006540:Herbicides / Q000494:pharmacology; D006358:Hot Temperature; D007668:Kidney / Q000166:cytology; D047029:Lactams, Macrocyclic; D008565:Membrane Proteins / Q000096:biosynthesis / Q000187:drug effects* / Q000235:genetics*; D011505:Protein-Tyrosine Kinases / Q000037:antagonists & inhibitors; D011809:Quinones / Q000494:pharmacology*; D012333:RNA, Messenger / Q000187:drug effects / Q000378:metabolism; D017828:Rifabutin / Q000031:analogs & derivatives; D029867:Xenopus Proteins; D014982:Xenopus laevis
chemical_list: D016227:Benzoquinones; D050890:HSP30 Heat-Shock Proteins; D018840:HSP70 Heat-Shock Proteins; D006360:Heat-Shock Proteins; D006540:Herbicides; D047029:Lactams, Macrocyclic; D008565:Membrane Proteins; D011809:Quinones; D012333:RNA, Messenger; D029867:Xenopus Proteins; C494391:hsp30 protein, Xenopus; D017828:Rifabutin; C020754:herbimycin; D011505:Protein-Tyrosine Kinases