High SDF-1 expression in HIV-1 carriers does not correlate with CD8+ T-cell-mediated suppression of viral replication.
Stromal cell-derived factor 1 (SDF-1) inhibits T-cell tropic (T-tropic) HIV-1 infection in vitro. In this study, we examined the regulatory role of SDF-1 on HIV-1 replication in peripheral blood mononuclear cells (PBMC) of HIV-infected individuals. We found that the amount of SDF-1 mRNA in freshly isolated PBMC of HIV-1 carriers was higher than in healthy donors. Moreover, PBMC from some asymptomatic carriers (ACs) exhibited high levels of SDF-1 mRNA expression. The level of SDF-1 expression in PBMC did not correlate with the magnitude of CD8+ T-cell-mediated suppression of HIV-1 among ACs SDF-1 inhibited HIV-1 replication at the viral entry step, whereas a single-cycle HIV-1 infection system showed that the major part of the CD8+ T-cell-mediated suppression occurs after intracellular penetration of the virus. Our results suggest that SDF-1 acts as a suppressor of virus replication in a CD8+ T-cell-independent mechanism in HIV-infected individuals.
Ohashi
T
T
Department of Immunotherapeutics, Tokyo Medical and Dental University, Japan. toha.impt@med.tmd.ac.jp
Arai
M
M
Kato
H
H
Kubo
M
M
Fujii
M
M
Yamamoto
N
N
Iwamoto
A
A
Kannagi
M
M
D001483:Base Sequence; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology*; D002353:Carrier State / Q000276:immunology* / Q000821:virology*; D054377:Chemokine CXCL12; D019743:Chemokines, CXC / Q000235:genetics* / Q000502:physiology; D017931:DNA Primers / Q000235:genetics; D015870:Gene Expression; D015658:HIV Infections / Q000276:immunology* / Q000821:virology*; D015497:HIV-1 / Q000276:immunology / Q000502:physiology*; D006801:Humans; D066298:In Vitro Techniques; D007963:Leukocytes, Mononuclear / Q000276:immunology / Q000378:metabolism / Q000821:virology; D012333:RNA, Messenger / Q000097:blood / Q000235:genetics; D014779:Virus Replication / Q000276:immunology
C515255:CXCL12 protein, human; D054377:Chemokine CXCL12; D019743:Chemokines, CXC; D017931:DNA Primers; D012333:RNA, Messenger