title: Summary of the first round analyses of the Second International Swine CD Workshop.
abstract: The reactivity of 176 monoclonal antibodies (mAb) submitted to the Second International Swine CD Workshop, together with 19 internal standards, was analyzed by flow cytometry on 16 different cell types as a means of establishing the proper cell subset for later detailed clustering analyses. The exact CD subset reactivity of the 19 internal standard mAb had been characterized in the First International Swine CD Workshop. The flow cytometric analyses resulted in 40 data sets which were then subjected to statistical clustering using the Leukocyte Typing Database IV (LTDB4) software. As result of this work, 22 clusters were defined. After review of these results, panels of mAb from the defined first round clusters were assigned to cell subsets. The respective mAb in those first round clusters were then distributed to subset group researchers for further examination during the second round of the workshop.
authors.affiliation: Federal Research Centre for Virus Diseases of Animals, Tübingen, Germany.
authors.forename: A
authors.initials: A
authors.lastname: Saalmüller
authors.forename: T
authors.initials: T
authors.lastname: Pauly
authors.forename: B
authors.initials: B
authors.lastname: Aasted
authors.forename: K T
authors.initials: KT
authors.lastname: Jensen
authors.forename: D H
authors.initials: DH
authors.lastname: Sachs
authors.forename: S
authors.initials: S
authors.lastname: Arn
authors.forename: W C
authors.initials: WC
authors.lastname: Davis
authors.forename: Y H
authors.initials: YH
authors.lastname: Park
authors.forename: K
authors.initials: K
authors.lastname: McCullough
authors.forename: A
authors.initials: A
authors.lastname: Summerfield
authors.forename: M
authors.initials: M
authors.lastname: Murtaugh
authors.forename: M S
authors.initials: MS
authors.lastname: Pampusch
authors.forename: K D
authors.initials: KD
authors.lastname: Burger
authors.forename: J
authors.initials: J
authors.lastname: Laber
authors.forename: J
authors.initials: J
authors.lastname: Nielsen
authors.forename: M D
authors.initials: MD
authors.lastname: Pescovitz
authors.forename: C
authors.initials: C
authors.lastname: Stokes
authors.forename: K
authors.initials: K
authors.lastname: Haverson
authors.forename: P
authors.initials: P
authors.lastname: Boyd
authors.forename: J K
authors.initials: JK
authors.lastname: Lunney
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000032:analysis*; D000937:Antigen-Antibody Reactions; D015703:Antigens, CD / Q000276:immunology*; D007963:Leukocytes, Mononuclear / Q000276:immunology; D013552:Swine / Q000276:immunology*
chemical_list: D000911:Antibodies, Monoclonal; D015703:Antigens, CD