Universal cytochrome b primers facilitate intraspecific studies in molluscan taxa.
We describe the construction of amplification primers designed to target a portion of the mitochondrial cytochrome b locus in a variety of molluscan taxa. Combinations of two sets of primers successfully amplified cytochrome b from several species of gastropods, bivalves, and cephalopods. Sequence analysis of these amplified products revealed nucleotide diversity in small samples within several of these taxa. We discuss the utility of these primer sets for studies of intraspecific phylogeny in mollusks and potentially other invertebrates.
Merritt
T J
TJ
Department of Biological Sciences, University of South Carolina, Columbia 29208, USA.
Shi
L
L
Chase
M C
MC
Rex
M A
MA
Etter
R J
RJ
Quattro
J M
JM
D000595:Amino Acid Sequence; D000818:Animals; D001181:Arthropods / Q000235:genetics; D001483:Base Sequence; D003573:Cytochrome b Group / Q000235:genetics*; D017931:DNA Primers; D004272:DNA, Mitochondrial / Q000235:genetics*; D008969:Molecular Sequence Data; D008974:Mollusca / Q000145:classification / Q000235:genetics*; D016133:Polymerase Chain Reaction / Q000379:methods; D017386:Sequence Homology, Amino Acid; D012689:Sequence Homology, Nucleic Acid
D003573:Cytochrome b Group; D017931:DNA Primers; D004272:DNA, Mitochondrial