Region-specific migration of embryonic glia grafted to the neonatal brain.
Cell fate determination and region-specific migration among neurons from the developing brain have been widely studied. Because similar attributes have been mostly unexplored in reference to glia, the present study has characterized the migratory responses of glia from diverse regions of the embryonic mouse brain after their transplantation to the brains of early postnatal (still developing) rats. Through the use of the mouse-specific, glial-specific marker M2, immunocytochemical processing of host tissues three to four weeks after transplantation revealed notable difference in the migratory patterns of phylogenetically diverse populations of glia. While glia from the ventral mesencephalon, cerebral cortex, and cerebellar neuroepithelium all showed a similar affinity for the nigropallidal tract after grafting to the internal capsule, only ventral mesencephalon-derived glia showed restricted migration toward and into the substantia nigra after transplantation to the thalamus or pontine tegmentum. These results suggest the presence of a highly favourable substrate for glial migration along developing fibre tracts, but, more importantly, indicates the potential for certain glia to respond to particular (region-specific) distal cues within the developing brain.
Gates
M A
MA
Wallenberg Neuroscience Center, Department of Physiology and Neuroscience, Lund University, Sweden.
Olsson
M
M
Bjerregaard
K
K
Björklund
A
A
D000818:Animals; D000831:Animals, Newborn / Q000502:physiology*; D001921:Brain / Q000166:cytology* / Q000254:growth & development; D016380:Brain Tissue Transplantation / Q000502:physiology*; D002465:Cell Movement / Q000502:physiology; D017690:Cell Transplantation / Q000502:physiology*; D016332:Fetal Tissue Transplantation / Q000502:physiology*; D007150:Immunohistochemistry; D051379:Mice; D009457:Neuroglia / Q000637:transplantation*; D011149:Pons / Q000166:cytology / Q000502:physiology; D051381:Rats; D013788:Thalamus / Q000166:cytology / Q000502:physiology