[A new technique for isolation of photoreceptor layer].
OBJECTIVE
If somebody wants to start the research of photoreceptor transplantation, first of all, the pure photoreceptor layer should be obtained. The pure photoreceptor is the key of retinal transplanting experiment for accurate analyses and good results. We present the pure photoreceptor layer isolation technique.


METHODS
A vibratome, a special machine for tissue and cell isolation, was used for retinal isolation and pure photoreceptor cells were harvested.


RESULTS
The pure photoreceptor cell layers of mice, rats, rabbits, monkeys and human have been obtained by this new technique.


CONCLUSIONS
By the technique, 100 per cent of pure photoreceptor cells could be isolated, and the manipulation is simple and reliable.
Wang
X
X
Department of Plastic Surgery, Third Hospital, Beijing Medical University.
Silverman
M
M
D000818:Animals; D006801:Humans; D008253:Macaca mulatta; D051379:Mice; D008867:Microtomy / Q000379:methods; D010786:Photoreceptor Cells / Q000166:cytology*; D011817:Rabbits; D051381:Rats