The sex-inducing pheromone and wounding trigger the same set of genes in the multicellular green alga Volvox.
The sex-inducing pheromone of the multicellular green alga Volvox carteri is a glycoprotein that triggers development of males and females at a concentration <10(-16) M. By differential screening of a cDNA library, two novel genes were identified that are transcribed under the control of this pheromone. Unexpectedly, one gene product was characterized as a lysozyme/chitinase, and the other gene product was shown to encode a polypeptide with a striking modular composition. This polypeptide has a cysteine protease domain separated by an extensin-like module from three repeats of a chitin binding domain. In higher plants, similar protein families are known to play an important role in defense against fungi. Indeed, we found that the same set of genes triggered by the sexual pheromone was also inducible in V. carteri by wounding.
Amon
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P
Lehrstuhl Biochemie I, Universität Regensburg, D-93053 Regensburg, Germany.
Haas
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Sumper
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M
D000595:Amino Acid Sequence; D002352:Carrier Proteins / Q000096:biosynthesis* / Q000737:chemistry / Q000235:genetics; D056655:Cathepsin H; D002403:Cathepsins / Q000737:chemistry; D002686:Chitin / Q000378:metabolism; D002688:Chitinases / Q000096:biosynthesis* / Q000737:chemistry / Q000235:genetics; D000460:Chlorophyta / Q000235:genetics / Q000502:physiology*; D003546:Cysteine Endopeptidases / Q000737:chemistry; D018076:DNA, Complementary; D018506:Gene Expression Regulation, Plant; D015723:Gene Library; D008969:Molecular Sequence Data; D009113:Muramidase / Q000737:chemistry; D010675:Pheromones / Q000502:physiology*; D012098:Reproduction / Q000235:genetics; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid; D014158:Transcription, Genetic
D002352:Carrier Proteins; D018076:DNA, Complementary; D010675:Pheromones; D002686:Chitin; D002688:Chitinases; D009113:Muramidase; D002403:Cathepsins; D003546:Cysteine Endopeptidases; D056655:Cathepsin H