title: A comparison of the biochemical composition of equine follicular fluid and serum at four different stages of the follicular cycle.
abstract: Samples of blood and follicular fluid were recovered from 27 Welsh Pony mares at 4 distinct stages of follicular development. Eighteen biochemical parameters were measured in each sample, including sodium, potassium, chloride, glucose, urea, creatinine, calcium, inorganic phosphate, total bilirubin, total protein, albumin, magnesium, triglyceride, total cholesterol, nonesterified fatty acids, alkaline phosphatase, gamma-glutamyltransferase and aspartate aminotransferase. The concentrations of progesterone, 17beta oestradiol and testosterone, pH and osmolarity, were also measured in all the follicular fluid samples. The concentrations of all proteins measured were lower in follicular fluid than serum whereas the reverse was true in the case of the lipids. Analysis of variance indicated that serum and follicular fluid concentrations of most of the parameters measured varied in parallel.
authors.affiliation: Irish Equine Centre, Johnstown, Naas, Co. Kildare.
authors.forename: A
authors.initials: A
authors.lastname: Collins
authors.forename: E
authors.initials: E
authors.lastname: Palmer
authors.forename: J
authors.initials: J
authors.lastname: Bézard
authors.forename: J
authors.initials: J
authors.lastname: Burke
authors.forename: G
authors.initials: G
authors.lastname: Duchamp
authors.forename: T
authors.initials: T
authors.lastname: Buckley
mesh_terms: D000818:Animals; D015331:Cohort Studies; D004958:Estradiol / Q000032:analysis; D004973:Estrus Synchronization; D005260:Female; D015571:Follicular Fluid / Q000737:chemistry*; D005947:Glucose / Q000032:analysis; D006736:Horses / Q000097:blood / Q000502:physiology*; D006863:Hydrogen-Ion Concentration; D008055:Lipids / Q000032:analysis; D009994:Osmolar Concentration; D006080:Ovarian Follicle / Q000502:physiology*; D011374:Progesterone / Q000032:analysis; D011506:Proteins / Q000032:analysis; D012016:Reference Values; D013739:Testosterone / Q000032:analysis
chemical_list: D008055:Lipids; D011506:Proteins; D013739:Testosterone; D011374:Progesterone; D004958:Estradiol; D005947:Glucose