title: Laminin-dependent integrin clustering with tyrosine-phosphorylated molecules in a Drosophila neuronal cell line.
abstract: To gain more insight into the molecular and cellular aspects of basement membranes during Drosophila morphogenesis, especially in neural development, we carried out cell biological screening to establish a cell culture system in which Drosophila cell-matrix interaction could be reconstituted. The screening showed that a Drosophila neuronal cell line, BG2-c6, established from the third-instar larval central nervous system, had a strong adhesion activity when purified Drosophila laminin was used as a substrate. Outgrowth of axon-like structures was stimulated on laminin. Histochemical analysis revealed clusters of integrin together with phosphotyrosine and alpha-actinin. These data indicate that the Drosophila integrin cascade triggered by the interaction between BG2-c6 and laminin was initiated at the integrin cluster with tyrosine-phosphorylated proteins, similar to the observations in vertebrate cells.
authors.affiliation: Exploratory Research for Advanced Technology, Japan Science and Technology Corporation, Tsukuba Research Consortium, Tokodai.
authors.forename: Y
authors.initials: Y
authors.lastname: Takagi
authors.forename: K
authors.initials: K
authors.lastname: Ui-Tei
authors.forename: T
authors.initials: T
authors.lastname: Miyake
authors.forename: S
authors.initials: S
authors.lastname: Hirohashi
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D004330:Drosophila / Q000166:cytology / Q000196:embryology*; D006651:Histocytochemistry; D019476:Insect Proteins / Q000378:metabolism; D016023:Integrins / Q000378:metabolism*; D007797:Laminin / Q000502:physiology*; D009474:Neurons / Q000737:chemistry / Q000378:metabolism*; D010766:Phosphorylation; D015398:Signal Transduction; D014443:Tyrosine / Q000378:metabolism*
chemical_list: D019476:Insect Proteins; D016023:Integrins; D007797:Laminin; D014443:Tyrosine