title: Codon reading properties of tRNA variants substituted within the anticodon loop.
abstract: Effects of base substitution within tRNA anticodon loop on the codon reading activities were quantitatively analyzed with the use of a set of unmodified tRNA molecules with GGA anticodon. The first (position 32) and the last (position 38) nucleotides of the anticodon loop of the wild-type molecule was changed from C32A38 to U32A38, U32G38, and C32G38. The codon reading activities of these variants relative to that of the wild type molecule were measured in a cell-free translation system. The reading of both the UCU and UCC codons were lower in all the three variants than in the wild-type molecule.
authors.affiliation: Department of Industrial Chemistry, Faculty of Technology, Chiba Institute of Technology, Japan.
authors.forename: R
authors.initials: R
authors.lastname: Ouchi
authors.forename: K
authors.initials: K
authors.lastname: Takai
authors.forename: S
authors.initials: S
authors.lastname: Yokoyama
authors.forename: H
authors.initials: H
authors.lastname: Takaku
mesh_terms: D000926:Anticodon / Q000737:chemistry / Q000235:genetics; D001483:Base Sequence; D003062:Codon / Q000737:chemistry / Q000235:genetics*; D004926:Escherichia coli / Q000235:genetics; D014644:Genetic Variation; D008958:Models, Molecular; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation; D017354:Point Mutation; D014176:Protein Biosynthesis; D012329:RNA, Bacterial / Q000737:chemistry / Q000235:genetics; D012333:RNA, Messenger / Q000096:biosynthesis / Q000737:chemistry; D012362:RNA, Transfer, Ser / Q000737:chemistry* / Q000235:genetics*; D013698:Templates, Genetic
chemical_list: D000926:Anticodon; D003062:Codon; D012329:RNA, Bacterial; D012333:RNA, Messenger; D012362:RNA, Transfer, Ser