Stabilities of internal rU-dG and rG-dT pairs in RNA/DNA hybrids.
We report stabilities of internal non-Watson-Crick rU-dG and rG-dT pairs in RNA/DNA hybrids. UV melting curves revealed that a hybrid containing internal rG-dT pair had about 2 kcal mol-1 greater stability in free energy change at 37 degrees C than hybrids with other mismatches, rA-dA and rU-dT, at the same position. We discuss why internal rG-dT as well as rU-dG is stable nearly Watson-Crick base pairs rather than the other mismatches.
Sugimoto
N
N
Department of Chemistry, Faculty of Science, Konan University, Kobe, Japan.
Yasumatsu
I
I
Fujimoto
M
M
D001482:Base Composition; D004247:DNA / Q000737:chemistry*; D003849:Deoxyguanosine; D006151:Guanosine; D008958:Models, Molecular; D009690:Nucleic Acid Conformation; D009691:Nucleic Acid Denaturation; D009692:Nucleic Acid Heteroduplexes; D009693:Nucleic Acid Hybridization; D009838:Oligodeoxyribonucleotides / Q000737:chemistry*; D009843:Oligoribonucleotides / Q000737:chemistry*; D012313:RNA / Q000737:chemistry*; D013816:Thermodynamics; D013936:Thymidine; D014529:Uridine
D009692:Nucleic Acid Heteroduplexes; D009838:Oligodeoxyribonucleotides; D009843:Oligoribonucleotides; D006151:Guanosine; D012313:RNA; D004247:DNA; D003849:Deoxyguanosine; D013936:Thymidine; D014529:Uridine