Chemoafferent degeneration and carotid body hypoplasia following chronic hyperoxia in newborn rats.
1. To define the role of environmental oxygen in regulating postnatal maturation of the carotid body afferent pathway, light and electron microscopic methods were used to compare chemoafferent neurone survival and carotid body development in newborn rats reared from birth in normoxia (21 % O2) or chronic hyperoxia (60 % O2). 2. Four weeks of chronic hyperoxia resulted in a significant 41 % decrease in the number of unmyelinated axons in the carotid sinus nerve, compared with age-matched normoxic controls. In contrast, the number of myelinated axons was unaffected by hyperoxic exposure. 3. Chemoafferent neurones, located in the glossopharyngeal petrosal ganglion, already exhibited degenerative changes following 1 week of hyperoxia from birth, indicating that even a relatively short hyperoxic exposure was sufficient to derange normal chemoafferent development. In contrast, no such changes were observed in the vagal nodose ganglion, demonstrating that the effect of high oxygen levels was specific to sensory neurones in the carotid body afferent pathway. Moreover, petrosal ganglion neurones were sensitive to hyperoxic exposure only during the early postnatal period. 4. Chemoafferent degeneration in chronically hyperoxic animals was accompanied by marked hypoplasia of the carotid body. In view of previous findings from our laboratory that chemoafferent neurones require trophic support from the carotid body for survival after birth, we propose that chemoafferent degeneration following chronic hyperoxia is due specifically to the loss of target tissue in the carotid body.
Erickson
J T
JT
Department of Neurosciences, Case Western Reserve University School of Medicine, 10900 Euclid Avenue, Cleveland, OH 44106, USA.
Mayer
C
C
Jawa
A
A
Ling
L
L
Olson
E B
EB
Vidruk
E H
EH
Mitchell
G S
GS
Katz
D M
DM
D000344:Afferent Pathways / Q000254:growth & development / Q000473:pathology*; D000818:Animals; D000831:Animals, Newborn; D001369:Axons / Q000473:pathology / Q000502:physiology / Q000648:ultrastructure; D002344:Carotid Body / Q000254:growth & development / Q000473:pathology*; D002470:Cell Survival; D002628:Chemoreceptor Cells / Q000473:pathology* / Q000502:physiology; D005260:Female; D017950:Ganglia, Sensory / Q000254:growth & development / Q000473:pathology; D005930:Glossopharyngeal Nerve / Q000254:growth & development / Q000473:pathology; D018496:Hyperoxia; D009410:Nerve Degeneration / Q000209:etiology*; D009474:Neurons / Q000473:pathology* / Q000502:physiology / Q000648:ultrastructure; D011247:Pregnancy; D011297:Prenatal Exposure Delayed Effects; D051381:Rats; D017207:Rats, Sprague-Dawley; D012016:Reference Values