Mouse chondroitin 6-sulfotransferase: molecular cloning, characterization and chromosomal mapping.
Chondroitin 6-sulfotransferase (C6ST) catalyzes the transfer of sulfate from 3'-phosphoadenosine 5'-phosphosulfate to position 6 of the N-acetylgalactosamine residue of chondroitin. Using chick C6ST cDNA as a probe, we cloned the cDNA of mouse C6ST. The mouse enzyme was predicted to be composed of 472 amino acids, and exhibited 71% sequence identity with the chicken enzyme. The mouse and chicken catalytic domains exposed to the luminal side exhibited 81% identity, while the homology of the remaining regions was less. Transfection and expression of the mouse cDNA in COS-7 cells yielded C6ST activity. Keratan sulfate sulfotransferase activity, which was simultaneously expressed, amounted to 3% of the C6ST activity, this value being significantly lower than that observed in the case of the chicken enzyme. Mouse C6ST mRNA was strongly expressed in the spleen, lung, and eye. In situ hybridization revealed that the transcript was localized in stromal cells in the marginal zone and red pulp of the spleen, and stromal cells in the bone marrow. Fluorescence in situ hybridization analysis revealed the gene is located in mouse chromosome 9.
Uchimura
K
K
Department of Biochemistry, Nagoya University School of Medicine, Japan.
Kadomatsu
K
K
Fan
Q W
QW
Muramatsu
H
H
Kurosawa
N
N
Kaname
T
T
Yamamura
K
K
Fukuta
M
M
Habuchi
O
O
Muramatsu
T
T
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D001853:Bone Marrow / Q000201:enzymology; D019556:COS Cells; D002460:Cell Line; D002645:Chickens; D002874:Chromosome Mapping; D003001:Cloning, Molecular; D015723:Gene Library; D007700:Kinetics; D051379:Mice / Q000235:genetics*; D008969:Molecular Sequence Data; D012333:RNA, Messenger / Q000096:biosynthesis; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000378:metabolism; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid; D013154:Spleen / Q000201:enzymology; D015238:Sulfotransferases / Q000096:biosynthesis* / Q000737:chemistry / Q000235:genetics*; D014158:Transcription, Genetic; D014162:Transfection; D000097811:Carbohydrate Sulfotransferases
D012333:RNA, Messenger; D011994:Recombinant Proteins; D015238:Sulfotransferases; D000097811:Carbohydrate Sulfotransferases