Alpha-1 antichymotrypsin levels are actively increased in normal colostrum.
BACKGROUND
It is well known that human colostrum has important antiinflammatory functions. The purpose of the current study was to determine antiprotease levels in colostrum and serum and to assess the importance of local synthesis and the electrophoretic differences in both locations.


METHODS
Five protease inhibitors were determined by radical immunodiffusion in colostrum and serum samples taken simultaneously from 50 healthy women, 36 to 72 hours after delivery.


RESULTS
Antithrombin II, inter-alpha trypsin inhibitor, and alpha-2 macroglobulin levels were undetectable in colostrum. Mean antitrypsin levels in colostrum were 6% of serum levels, but colostrum alpha-1 antichymotrypsin was higher than expected (0.39+/-0.34 g/l) in relation according to the albumin passive transport, and their mean value was 41% of serum levels. Colostrum antichymotrypsin levels did not correlate with serum antichymotrypsin levels or with colostrum albumin levels. The antichymotrypsin molecule in colostrum had a slower electrophoretic mobility when compared with that of serum antichymotrypsin, and it showed a different pattern in Western blot analysis, with a predominating 80-kDa molecule.


CONCLUSIONS
Although the origin of colostral antichymotrypsin is unclear, local production in breast epithelial cells is likely. Antichymotrypsin is increased in colostrum, and its molecule has some characteristic differences, suggesting that it has an important and specific role in infant nutrition during breast milk feeding.
Urueña
C
C
Department of Pediatrics, University of Valladolid, Spain.
Tellería
J J
JJ
Blanco-Quirós
A
A
Arranz
E
E
Gomez-Carrasco
J A
JA
D003126:Colostrum / Q000378:metabolism*; D004587:Electrophoresis, Agar Gel; D004591:Electrophoresis, Polyacrylamide Gel; D005260:Female; D006801:Humans; D007123:Immunoelectrophoresis, Two-Dimensional; D012016:Reference Values; D012709:Serum Albumin / Q000378:metabolism; D000514:alpha 1-Antichymotrypsin / Q000097:blood / Q000378:metabolism*
D012709:Serum Albumin; D000514:alpha 1-Antichymotrypsin