title: Structural study on silkworm eclosion hormone fragment (1-34) in solution by proton nuclear magnetic resonance spectroscopy.
abstract: Eclosion hormone (EH) is a neuropeptide hormone which controls the ecdysis behavior in insect. The three dimensional structure of the N-terminal fragment (1-34) of the eclosion hormone which was predicted to contain a compact region crucial for the EH activity was studied in 50% d3-trifluoroethanol(TFE)/50% H2O at pH 3 and 298 K by 1H NMR spectroscopy with the combined use of distance geometry and molecular dynamics calculations. NMR results indicated that the fragment actually assumes an alpha-helix between Ala10 and Gln20, but no rigid structure is present from Cys21 through the C-terminus and for the N-terminal region (Ser1-Asp9). The elucidated structure was compared with the predicted structure of the native EH for the further development of the design of the insecticide.
authors.affiliation: International Research Laboratories, Ciba-Geigy Japan Ltd., Japan.
authors.forename: A Y
authors.initials: AY
authors.lastname: Nosaka
authors.forename: K
authors.initials: K
authors.lastname: Kanaori
authors.forename: I
authors.initials: I
authors.lastname: Umemura
authors.forename: M
authors.initials: M
authors.lastname: Takai
authors.forename: N
authors.initials: N
authors.lastname: Fujita
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D012831:Bombyx / Q000737:chemistry* / Q000502:physiology; D002942:Circular Dichroism; D006152:Cyclic GMP / Q000032:analysis; D005583:Fourier Analysis; D007301:Insect Hormones / Q000737:chemistry* / Q000494:pharmacology; D009682:Magnetic Resonance Spectroscopy; D008969:Molecular Sequence Data; D018978:Molting / Q000187:drug effects; D009479:Neuropeptides / Q000737:chemistry* / Q000494:pharmacology; D017434:Protein Structure, Tertiary
chemical_list: D007301:Insect Hormones; D009479:Neuropeptides; C042645:eclosion hormone; D006152:Cyclic GMP