Structure and expression of activin genes in rainbow trout.
Activins are dimeric members of the transforming growth factor-beta (TGF-beta) superfamily. By using the polymerase chain reaction (PCR), we have cloned and sequenced activin beta A and beta B genes encoding the mature region of the peptides from the rainbow trout genomic DNA. Two forms of beta A and a single form of beta B-subunits were found. There is high identity with mammalian counterparts; the two rainbow trout beta A-subunits have more than 75% nucleotide sequence identity with the human beta A-subunit, and the beta B-subunit had 82% sequence identity with the human beta B-subunit. Expression of rainbow trout activin genes was examined by reverse transcription-PCR (RT-PCR). The major expression tissue of rainbow trout activin was ovary and brain at the messenger RNA level, and the major expression subunit of rainbow trout activin was the beta B subunit.
Tada
T
T
Department of Aquatic Biosciences, Tokyo University of Fisheries, Japan.
Hirono
I
I
Aoki
T
T
Takashima
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F
D028341:Activins; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D015139:Blotting, Southern; D003001:Cloning, Molecular; D017931:DNA Primers; D018076:DNA, Complementary / Q000235:genetics; D015870:Gene Expression; D016678:Genome; D007265:Inhibins / Q000096:biosynthesis / Q000235:genetics*; D008969:Molecular Sequence Data; D017686:Oncorhynchus mykiss / Q000235:genetics*; D016133:Polymerase Chain Reaction; D012313:RNA / Q000235:genetics; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D014018:Tissue Distribution; D016212:Transforming Growth Factor beta / Q000235:genetics
D017931:DNA Primers; D018076:DNA, Complementary; D016212:Transforming Growth Factor beta; D028341:Activins; D007265:Inhibins; D012313:RNA