Characterization of two Sp1 binding sites of the human sex determining SRY promoter.
To investigate the molecular basis of the human SRY gene regulation, we have examined the significance of two potential binding sites for the transcription factor Sp1 (Sp1A: -124 to -131 and Sp1B: -147 to -154) by DNase I footprinting and gel mobility shift assays. Cotransfection experiments in Drosophila SL2 cells implicated Sp1 protein in the transcriptional activation of the SRY promoter.
Desclozeaux
M
M
Centre de Recherche de Biochimie Macromoléculaire, ERS155 CNRS, Montpellier, France.
Poulat
F
F
de Santa Barbara
P
P
Soullier
S
S
Jay
P
P
Berta
P
P
Boizet-Bonhoure
B
B
D000818:Animals; D001483:Base Sequence; D001665:Binding Sites / Q000235:genetics; D002460:Cell Line; D004247:DNA / Q000235:genetics / Q000378:metabolism; D018983:DNA Footprinting; D004268:DNA-Binding Proteins / Q000235:genetics*; D003850:Deoxyribonuclease I; D004330:Drosophila; D005260:Female; D006801:Humans; D008297:Male; D009687:Nuclear Proteins; D011401:Promoter Regions, Genetic; D019849:Sex Determination Processes; D051876:Sex-Determining Region Y Protein; D016329:Sp1 Transcription Factor / Q000378:metabolism*; D014157:Transcription Factors; D015533:Transcriptional Activation; D014162:Transfection
D004268:DNA-Binding Proteins; D009687:Nuclear Proteins; C497745:SRY protein, human; D051876:Sex-Determining Region Y Protein; D016329:Sp1 Transcription Factor; D014157:Transcription Factors; D004247:DNA; D003850:Deoxyribonuclease I