title: Evaluation of the analytical performance of an enzyme immunometric assay (EIA) designed to measure endogenous thyroid-stimulating hormone (TSH) in canine serum samples.
abstract: The analytical performance of a commercially available enzyme immunometric assay (EIA) designed to measure endogenous thyroid-stimulating hormone (cTSH) concentration in canine serum samples was evaluated. The precision of the assay was acceptable with intra- and inter-assay coefficients of variation (CV) of 5 and 11%, respectively. The assay maintained linearity under dilution and recovered added cTSH in a linear and proportional manner. The limit of detection (0.03 microgram/L) was well below the values measured in serum from dogs with primary hypothyroidism (median value 1.52 micrograms/L). Further, the assay clearly distinguished dogs with primary hypothyroidism from clinically healthy dogs (P = 0.0004). Based on these findings, it was concluded that the analytical performance of the assay was acceptable.
authors.affiliation: Department of Clinical Studies, Royal Veterinary and Agricultural University, Frederiksberg, Denmark.
authors.forename: L
authors.initials: L
authors.lastname: Iversen
authors.forename: R
authors.initials: R
authors.lastname: Høier
authors.forename: A L
authors.initials: AL
authors.lastname: Jensen
authors.forename: M
authors.initials: M
authors.lastname: Skydsgaard
authors.forename: J
authors.initials: J
authors.lastname: Koch
mesh_terms: D000818:Animals; D004283:Dog Diseases; D004285:Dogs / Q000097:blood*; D005260:Female; D007037:Hypothyroidism / Q000097:blood / Q000175:diagnosis / Q000662:veterinary*; D007124:Immunoenzyme Techniques / Q000662:veterinary; D008297:Male; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013972:Thyrotropin / Q000097:blood*
chemical_list: D013972:Thyrotropin