title: Mutational analysis of the phosphate-binding loop of Rhizobium meliloti DctD, a sigma54-dependent activator.
abstract: The phosphate-binding loop of sigma54-dependent activators is thought to participate in ATP binding and/or hydrolysis. Alanine substitutions at positions 3, 4, 6, 7, and 8 of this motif in Rhizobium meliloti DctD disrupted transcriptional activation and ATP hydrolysis. Interestingly, substitution of alanine at position 7 also affected DNA binding.
authors.affiliation: Department of Microbiology, University of Georgia, Athens, Georgia 30602, USA.
authors.forename: Y
authors.initials: Y
authors.lastname: Gao
authors.forename: Y K
authors.initials: YK
authors.lastname: Wang
authors.forename: T R
authors.initials: TR
authors.lastname: Hoover
mesh_terms: D000255:Adenosine Triphosphate / Q000378:metabolism; D001426:Bacterial Proteins; D004268:DNA-Binding Proteins; D012321:DNA-Directed RNA Polymerases / Q000235:genetics* / Q000378:metabolism; D006868:Hydrolysis; D009154:Mutation; D011487:Protein Conformation; D051877:RNA Polymerase Sigma 54; D012808:Sigma Factor / Q000235:genetics* / Q000378:metabolism; D016962:Sinorhizobium meliloti / Q000235:genetics* / Q000378:metabolism; D014157:Transcription Factors / Q000235:genetics* / Q000378:metabolism
chemical_list: D001426:Bacterial Proteins; D004268:DNA-Binding Proteins; D012808:Sigma Factor; D014157:Transcription Factors; C073788:dctD protein, Rhizobium meliloti; D000255:Adenosine Triphosphate; D012321:DNA-Directed RNA Polymerases; D051877:RNA Polymerase Sigma 54