Enzyme linked immunosorbent assay for potency testing of vaccines containing Clostridium perfringens type D epsilon-toxoid.
For ethical, economic and technical reasons in vivo assays need to be replaced in routine laboratory procedures. Based on a method which is already accepted by the British authorities, an indirect ELISA has been developed and evaluated for Clostridium perfringens type D-containing vaccines. Individual and pooled sera of vaccinated rabbits were tested at a single dilution level, the results transferred into IU/ml, and compared with the conventional toxin neutralization test in mice. The ELISA was found to give reproducible estimates of antitoxin levels and showed good correlation with the conventional in vivo test in mice.
Pfahler
W
W
Instituto Rosenbusch de Biología Experimental Agropecuaria, Buenos Aires, Argentina.
Bisesti
E
E
Pereyra
J B
JB
Iribarren
F
F
D000818:Animals; D000942:Antigens, Bacterial / Q000097:blood / Q000276:immunology; D001427:Bacterial Toxins / Q000097:blood / Q000276:immunology*; D001428:Bacterial Vaccines / Q000032:analysis / Q000592:standards*; D002138:Calibration; D003016:Clostridium perfringens / Q000276:immunology*; D003168:Complement Fixation Tests; D004591:Electrophoresis, Polyacrylamide Gel / Q000662:veterinary; D004797:Enzyme-Linked Immunosorbent Assay / Q000662:veterinary*; D051379:Mice; D011817:Rabbits; D012015:Reference Standards; D015203:Reproducibility of Results; D014121:Toxoids / Q000276:immunology*
D000942:Antigens, Bacterial; D001427:Bacterial Toxins; D001428:Bacterial Vaccines; C039811:Clostridium perfringens epsilon-toxin; D014121:Toxoids