title: Immunohistochemical detection of ribosomal transcription factor UBF: diagnostic value in malignant specimens.
abstract: The nucleolar organizer regions (NORs) of human chromosome can be identified in interphase and mitotic cells by localization of some intrinsic components such as the associated enzyme RNA polymerase I. A new sensitive staining method for NORs is described using a specific antibody to the ribosomal transcription factor UBF. By indirect immunofluorescence and enzyme-labelling methods, NORs stained in benign and malignant cells from a variety of tissues with monospecific anti-UBF serum showed significant morphological differences which correlated well with histopathological evaluation. The number of NORs per cell in malignant preparations increased significantly. Furthermore, the staining of a NOR protein component such as UBF appears to be as sensitive as the silver-staining technique (AgNOR) and might be a better alternative for detecting ribosomal activity in malignant tissues.
authors.affiliation: Servicio de Anatomia Patológica, Hospital Clínico, Cádiz, Spain.
authors.forename: A
authors.initials: A
authors.lastname: Torres-Montaner
authors.forename: J
authors.initials: J
authors.lastname: Bolivar
authors.forename: M
authors.initials: M
authors.lastname: Ortiz
authors.forename: M M
authors.initials: MM
authors.lastname: Valdivia
mesh_terms: D014408:Biomarkers, Tumor / Q000378:metabolism*; D004268:DNA-Binding Proteins / Q000276:immunology / Q000378:metabolism*; D005455:Fluorescent Antibody Technique; D006801:Humans; D007106:Immune Sera; D007124:Immunoenzyme Techniques; D008223:Lymphoma / Q000175:diagnosis / Q000378:metabolism; D009363:Neoplasm Proteins / Q000378:metabolism*; D009369:Neoplasms / Q000175:diagnosis* / Q000378:metabolism; D009697:Nucleolus Organizer Region / Q000378:metabolism*; D035463:Pol1 Transcription Initiation Complex Proteins; D016622:Silver Staining; D014157:Transcription Factors / Q000276:immunology / Q000378:metabolism*
chemical_list: D014408:Biomarkers, Tumor; D004268:DNA-Binding Proteins; D007106:Immune Sera; D009363:Neoplasm Proteins; D035463:Pol1 Transcription Initiation Complex Proteins; D014157:Transcription Factors; C063844:transcription factor UBF