Preparation & standardization of the goat lung surfactant extract.
Exogenous goat lung surfactant extract (GLSE) was obtained by chloroform-methanol extraction of the centrifuged lung lavage. Four lungs provided around 250 mg of surfactant. GLSE has a phospholipid content of 50 percent, cholesterol of 1.5 percent and protein content under 1 percent. The preparation was bacteriologically sterile and had a shelf life of at least one month at 4 degrees C. Endotracheal administration of surfactant (50 mg/kg) in a rat lung model rendered surfactant deficient by saline lavage, restored the compliance characteristics to normal, thus documenting biological activity of the preparation. The cost of a course of 2 doses of surfactant replacement therapy was estimated to be as low as Rs 500/-. The study opens up the possibility of surfactant replacement therapy for the management of neonatal respiratory distress syndrome in India by an indigenous product at an affordable cost.
Narayanan
S
S
Department of Paediatrics, All India Institute of Medical Sciences, New Delhi.
Paul
V K
VK
Singh
M
M
Pande
J N
JN
Rao
D N
DN
Vijayaraghvan
M
M
Deorari
A K
AK
D000818:Animals; D006041:Goats; D008168:Lung / Q000187:drug effects*; D011663:Pulmonary Surfactants / Q000302:isolation & purification* / Q000494:pharmacology; D051381:Rats; D012015:Reference Standards; D014020:Tissue Extracts / Q000494:pharmacology*
D011663:Pulmonary Surfactants; D014020:Tissue Extracts