The influence of dietary sugars and starch on the establishment of Streptococcus mutans and Actinomyces viscosus in dental plaque of specific pathogen-free rats.
The establishment of S. mutans together with A. viscosus was investigated in dental plaque of specific pathogen-free (SPF) rats fed different carbohydrate diets. Two Tanzanian S.mutans strains MM3 and MM24 and one Tanzanian A. viscosus strain MM13 were used for this purpose. The basic diet consisting of 32% skim-milk, 7% yeast extract and 1% soy bean oil was supplemented with either 10% lactose and 50% corn flour, or 10% glucose and 50% corn flour, or 60% amylum or 60% wheat flour. S. mutans and A. viscosus were enumerated twenty days after inoculation. S. mutans counts were high irrespective of the dietary regime. A. viscosus counts in the glucose, lactose and amylum groups were of the same magnitude and significantly higher than those of the wheat flour group. The hypothesis that the establishment of S. mutans in sucrose free diets could be facilitated by the extracellular polysaccharides produced by A. viscosus was not supported by the present data. The finding that S. mutans can establish in high number in dental plaque of SPF rats in the absence of sucrose corroborates previous reports indicating high S. mutans counts in African populations with a low sucrose intake.
Matee
M I
MI
Department of Microbiology and Immunology, Muhimbili University College of Health Sciences, Dar-es-Salaam, Tanzania.
Mikx
F H
FH
Maselle
S Y
SY
Van Palenstein Helderman
W H
WH
D016924:Actinomyces viscosus / Q000254:growth & development*; D000704:Analysis of Variance; D000818:Animals; D015169:Colony Count, Microbial; D003773:Dental Plaque / Q000378:metabolism / Q000382:microbiology*; D004040:Dietary Carbohydrates / Q000008:administration & dosage*; D019422:Dietary Sucrose / Q000008:administration & dosage*; D005260:Female; D008297:Male; D011897:Random Allocation; D051381:Rats; D017208:Rats, Wistar; D013047:Specific Pathogen-Free Organisms; D013213:Starch / Q000008:administration & dosage*; D013295:Streptococcus mutans / Q000254:growth & development*; D013997:Time Factors
D004040:Dietary Carbohydrates; D019422:Dietary Sucrose; D013213:Starch