Central distribution and function of 5-HT6 receptor subtype in the rat brain.
The purpose of the present study was to elucidate the distribution of 5-HT6 receptor mRNA and possible physiological functions of the 5-HT6 receptor subtype using antisense oligonucleotides (AOs) in rats. Continuous intracerebroventricular injection of AOs caused a 30% reduction in the number of [3H]-lysergic acid diethylamide binding sites. Conditioned fear stress-induced increase in 5-HT release from the prefrontal cortex was significantly inhibited by treatment with AOs. The present study suggests that 5-HT6 receptors may be functionally expressed in the brain, where one of the functions appears to be in the mediation of certain anxiety disorders.
Yoshioka
M
M
First Department of Pharmacology, Hokkaido University School of Medicine, Sapporo, Japan.
Matsumoto
M
M
Togashi
H
H
Mori
K
K
Saito
H
H
D000818:Animals; D001921:Brain / Q000187:drug effects / Q000378:metabolism / Q000648:ultrastructure*; D017403:In Situ Hybridization; D007276:Injections, Intraventricular; D008297:Male; D017551:Microdialysis; D016376:Oligonucleotides, Antisense / Q000494:pharmacology; D017397:Prefrontal Cortex / Q000187:drug effects / Q000378:metabolism / Q000648:ultrastructure; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D011985:Receptors, Serotonin / Q000096:biosynthesis* / Q000378:metabolism; D012701:Serotonin / Q000378:metabolism; D013312:Stress, Physiological / Q000378:metabolism
D016376:Oligonucleotides, Antisense; D012333:RNA, Messenger; D011985:Receptors, Serotonin; C097216:serotonin 6 receptor; D012701:Serotonin