Intermediate in reaction of 2'-deoxycytidine with nitrous acid.
Using reversed phase (RP) HPLC analysis, we reinvestigated the reaction of dCyd with nitrous acid. When 10 mM 2'-deoxycytidine (dCyd) was incubated with 100 mM NaNO2 in 1.0 M acetate buffer (pH 3.7) at 37 degrees C, an unknown product (referred to compound 1) was detected. The time course of the yield of compound 1 showed profile characteristic of an intermediate. Under the physiological conditions, compound 1 was converted exclusively to dUrd with a life time of ca. 300 h. Elucidation of genotoxic effects of compound 1 may be an important subject of the future study.
Suzuki
T
T
Institute of Advanced Energy, Kyoto University, Uji, Japan.
Nakamura
T
T
Kanaori
K
K
Tajima
K
K
Makino
K
K
D002851:Chromatography, High Pressure Liquid / Q000379:methods; D003841:Deoxycytidine / Q000031:analogs & derivatives / Q000737:chemistry*; D003857:Deoxyuridine / Q000032:analysis; D007202:Indicators and Reagents; D007700:Kinetics; D009153:Mutagens; D009603:Nitroso Compounds / Q000737:chemistry; D009608:Nitrous Acid / Q000737:chemistry*
C113091:2'-deoxy-N(4)-nitrosocytidine; D007202:Indicators and Reagents; D009153:Mutagens; D009603:Nitroso Compounds; D003841:Deoxycytidine; D009608:Nitrous Acid; D003857:Deoxyuridine