Developmental expression of messenger RNA levels of the alpha subunit of the GTP-binding protein, Gz, in the mouse nervous system.
There has been recent evidence that Gz may play a role in the transmission of the neurotrophic signal from nerve terminals to the cell bodies [Johanson, S.O., Crouch, M.F., Hendry, I.A., Signal transduction from membrane to nucleus: the special case for neurons, Neurochem. Res. 21 (1996) 779-785]. We examined the developmental expression of the alpha subunit of Gz (Gzalpha) in the peripheral and central nervous systems of the mouse. Our laboratory has developed a quantitative reverse transcription-polymerase chain reaction (RT-PCR) for Gzalpha which makes use of a fragment of the PCR product shortened by 107 base pairs creating a standard which mimics the original RNA. Serial dilutions of the mouse RNA with a constant concentration of mimic RNA were made and the point where equal amounts of product are formed allows accurate measurement of Gzalpha mRNA in the tissue. We have demonstrated that in the developing mouse superior cervical ganglion (SCG), dorsal root ganglion (DRG) and trigeminal ganglion the expression of Gzalpha mRNA is highest perinatally. From 3 weeks of age, in all tissues with the exception of the SCG, Gzalpha mRNA levels fall to lower levels in the adult animal. The developmental pattern of expression of Gzalpha in both the cerebellum and the brain differs from the peripheral nervous system. In the cerebellum, Gzalpha mRNA expression is highest around birth and in the brain it is highest around third postnatal week and then the levels decline as adulthood is approached. These results suggest that the highest level of Gzalpha mRNA is expressed at the time when target tissue innervation is occurring. This further strengthens the hypothesis that Gzalpha is important in the transfer of information from target tissues to the innervating nerve cells.
Kelleher
K L
KL
Division of Neuroscience, John Curtin School of Medical Research, Australian National University, Box 334, Canberra, A.C.T. 2601, Australia. kim.kelleher@anu.edu.au
Matthaei
K I
KI
Leck
K J
KJ
Hendry
I A
IA
D000818:Animals; D002490:Central Nervous System / Q000254:growth & development / Q000378:metabolism; D005260:Female; D044385:GTP-Binding Protein alpha Subunits; D019204:GTP-Binding Proteins / Q000096:biosynthesis* / Q000235:genetics*; D005727:Ganglia, Spinal / Q000254:growth & development / Q000378:metabolism; D018507:Gene Expression Regulation, Developmental / Q000502:physiology*; D020962:Heterotrimeric GTP-Binding Proteins; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D009420:Nervous System / Q000254:growth & development* / Q000378:metabolism*; D017933:Peripheral Nervous System / Q000254:growth & development / Q000378:metabolism; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000096:biosynthesis* / Q000235:genetics*; D015398:Signal Transduction; D017783:Superior Cervical Ganglion / Q000254:growth & development / Q000378:metabolism; D012668:Trigeminal Ganglion / Q000254:growth & development / Q000378:metabolism
D044385:GTP-Binding Protein alpha Subunits; C490964:Gnaz protein, mouse; D012333:RNA, Messenger; D019204:GTP-Binding Proteins; D020962:Heterotrimeric GTP-Binding Proteins