Homogeneous catalyst for DNA hydrolysis (4). Efficient DNA hydrolysis by lanthanide--saccharide complexes.
Homogeneous and neutral solutions are prepared by mixing Ce(NH4)2(NO3)6 with either isomaltose, melibiose, gentiobiose, palatinose, mannitol, sorbitol, galactitol, or glucamine in pH 7 hepes buffer ([Ce(IV)]0/[monomeric residue of saccharide]0 = 1). In contrast, amylose, cyclodextrins, maltose, glucose, and fructose provide only heterogeneous mixtures. The homogeneous solution of 1:1 Ce(IV)/glucamine system is active for DNA hydrolysis: the pseudo-first-order rate constant for the hydrolysis of thymidylyl(3'-->5')thymidine at pH 7.0 and 50 degrees C is 0.010 h-1, when [Ce(IV)]0 = [glucamine]0 = 10 mmol dm-3. The DNA-hydrolyzing activity decreases in the following order: glucamine > isomaltose, melibiose, gentiobiose >> palatinose, mannitol, sorbitol, galactitol.
Sumaoka
J
J
Department of Chemistry and Biotechnology, Graduate School of Engineering, University of Tokyo, Japan.
Kajimura
A
A
Komiyama
M
M
D002384:Catalysis; D002563:Cerium; D004247:DNA / Q000737:chemistry*; D004187:Disaccharides; D006868:Hydrolysis; D007700:Kinetics; D008674:Metals, Rare Earth; D009005:Monosaccharides; D013329:Structure-Activity Relationship; D013936:Thymidine
D004187:Disaccharides; D008674:Metals, Rare Earth; D009005:Monosaccharides; D002563:Cerium; D004247:DNA; D013936:Thymidine