title: Anti-beta 2-glycoprotein I antibodies bind to central nervous system.
abstract: Anti-beta 2-GPI antibodies (a beta 2-GPI) were found in serum from patients with anti-phospholipid syndrome (APS) and/or systemic lupus erythematosus (SLE). Since a beta 2-GPI are often found in patients with anti-cardiolipin antibodies (aCL), their role in thrombosis as well as other central nervous system (CNS) manifestations in APS is unclear. We, therefore, investigated whether affinity-purified a beta 2-GPI bind the CNS. Astrocyte and neuron cell lines and histological sections were used as CNS substrates. Indirect immunofluorescence and/or streptavidin-biotin-peroxidase techniques revealed that astrocytes, neurons and vascular endothelium were bound by purified a beta 2-GPI (mouse monoclonal, rabbit polyclonal, human serum Ig a beta 2-GPI). This suggests a potential role for a beta 2-GPI in the CNS damage, as a beta 2-GPI might contribute to CNS pathology by either a direct interaction with astrocytes and neurons or an interaction with cerebral vascular endothelial cells. CNS immunoreaction was also demonstrated using six a beta 2-GPI-positive sera from patients (four with neurological manifestations). No binding to CNS was seen using a beta 2-GPI-negative sera, i.e. five from SLE patients (two with CNS involvement) and six healthy donors, or a monoclonal aCL without a beta 2-GPI immunoreactivity. Thus, the CNS reactivity by the a beta 2-GPI-positive sera appears specifically due to a beta 2-GPI and independent from aCL. Because of the presence of aCL in all patient sera, and the CNS involvement in three control patients, it is not possible to attribute a direct role for a beta 2-GPI in neurological diseases in this study.
authors.affiliation: Dipartimento di Science Neurologiche, Università La Sapienza, Rome, Italy.
authors.forename: B
authors.initials: B
authors.lastname: Caronti
authors.forename: V
authors.initials: V
authors.lastname: Pittoni
authors.forename: G
authors.initials: G
authors.lastname: Palladini
authors.forename: G
authors.initials: G
authors.lastname: Valesini
mesh_terms: D000818:Animals; D016736:Antiphospholipid Syndrome / Q000276:immunology; D001323:Autoantibodies / Q000378:metabolism*; D001666:Binding Sites, Antibody; D001921:Brain / Q000378:metabolism / Q000473:pathology; D002460:Cell Line; D002490:Central Nervous System / Q000276:immunology* / Q000378:metabolism*; D006023:Glycoproteins / Q000276:immunology*; D000882:Haplorhini; D006801:Humans; D007150:Immunohistochemistry; D011817:Rabbits; D051381:Rats; D053482:beta 2-Glycoprotein I
chemical_list: D001323:Autoantibodies; D006023:Glycoproteins; D053482:beta 2-Glycoprotein I