title: [Comparison of local antigen-specific IgA responses with serum IgA response after intrapulmonary antigen immunization in healthy subjects].
abstract: OBJECTIVE
  To observe local lung and serum antigen-specific IgA response after local lung antigen immunization (LLI).
  
  
  METHODS
  11 healthy subjects were immunized with keyhole limpet hemocyanine (KLH) through bronchoscopy. 10-14 days after immunization, IgA responses were obversed in bronchoalveolar lavage fluids (BALF) and serum.
  
  
  RESULTS
  LLI induced anti-KLH IgA response in immunized BALF and serum, and both were polymeric IgA. Anti-KLH IgA2/IgA1 ratios were greater in immunized BALF than in serum. Anti-KLH IgA activity in immunized BALF was associated with secretory component (SC), but in serum was not correlated with SC.
  
  
  CONCLUSIONS
  LLI could induce local antigen-specific IgA production. There were markedly differences between the structure and subclass distribution of antigen-specific IgA in immunized BALF and serum.
authors.affiliation: Zhejiang Medical University Affiliated Second Hospital, Hangzhou.
authors.forename: H
authors.initials: H
authors.lastname: Shen
authors.forename: Y
authors.initials: Y
authors.lastname: Hua
authors.forename: F
authors.initials: F
authors.lastname: Liu
mesh_terms: D000276:Adjuvants, Immunologic; D000328:Adult; D001704:Biopolymers / Q000378:metabolism; D001992:Bronchoalveolar Lavage Fluid / Q000276:immunology; D005260:Female; D006433:Hemocyanins / Q000276:immunology; D006801:Humans; D007070:Immunoglobulin A / Q000145:classification / Q000276:immunology / Q000378:metabolism*; D008168:Lung / Q000276:immunology*; D008297:Male
chemical_list: D000276:Adjuvants, Immunologic; D001704:Biopolymers; D007070:Immunoglobulin A; C034639:polymeric IgA; D006433:Hemocyanins; C032808:keyhole-limpet hemocyanin