Assay of acyclovir in human skin layers by high-performance liquid chromatography.
This paper describes an assay procedure for acyclovir quantification in human skin after in vitro transdermal transport experiments. The procedure employs warm distilled water for acyclovir (ACV) extraction and high-performance liquid chromatography (HPLC) as analytical method. The procedure has good reproducibility, sensitivity and specificity, resulting in a reliable method for biopharmaceutical studies of ACV distribution in skin tissue. The chromatographic conditions set up, using distilled water as mobile phase, makes the analytical procedure simple and easy to perform.
Volpato
N M
NM
Dipartimento Farmaceutico, Università di Parma, Italy.
Santi
P
P
Laureri
C
C
Colombo
P
P
D000212:Acyclovir / Q000032:analysis*; D002138:Calibration; D002851:Chromatography, High Pressure Liquid; D006801:Humans; D007478:Iontophoresis; D015203:Reproducibility of Results; D012867:Skin / Q000737:chemistry*; D013056:Spectrophotometry, Ultraviolet
D000212:Acyclovir