title: Phenylethanolamine-N-methyltransferase - immunoreactive nerve terminals afferent to the mouse substantia nigra.
abstract: In the substantia nigra pars compacta, many phenylethanolamine-N-methyltransferase immunoreactive (PNMT-ir) terminals as well as serotonin-ir terminals were observed for the first time to be very closely situated to the tyrosine hydroxylase (TH)-ir, aromatic L-amino acid decarboxylase-ir, and GTP cyclohydrolase I (GCH)-ir dopaminergic cells [Nagatsu, I., Arai, R., Sakai, M., Yamawaki, Y., Takeuchi, T., Karasawa, N. and Nagatsu, T., Neurosci. Lett., 224 (1997) 185-188]. Immunohistochemical colocalization of TH with GCH or PNMT in the somata and dendrites of TH-positive neurons in the rostral ventrolateral reticular formation of the medulla oblongata (C1 region, [Hokfelt, T., Fuxe, K., Goldstein, M. and Johansson, O., Brain Res., 66 (1974) 235-251]) was proved by a double-labeling immunofluorescence method with a confocal laser-scanning microscope, indicating that the neurons are adrenergic. These results suggest that dopaminergic neurons in the substantia nigra receive PNMT-ir, adrenergic afferents from the C1 region of the medulla oblongata.
authors.affiliation: Department of Anatomy, School of Medicine, Fujita Health University, Toyoake, Aichi, Japan.
authors.forename: I
authors.initials: I
authors.lastname: Nagatsu
authors.forename: K
authors.initials: K
authors.lastname: Ikemoto
authors.forename: T
authors.initials: T
authors.lastname: Takeuchi
authors.forename: R
authors.initials: R
authors.lastname: Arai
authors.forename: N
authors.initials: N
authors.lastname: Karasawa
authors.forename: T
authors.initials: T
authors.lastname: Fujii
authors.forename: T
authors.initials: T
authors.lastname: Nagatsu
mesh_terms: D000818:Animals; D001142:Aromatic-L-Amino-Acid Decarboxylases / Q000378:metabolism; D004298:Dopamine / Q000502:physiology; D019084:Fluorescent Antibody Technique, Indirect; D006136:GTP Cyclohydrolase / Q000378:metabolism; D007150:Immunohistochemistry; D051379:Mice; D018613:Microscopy, Confocal; D009411:Nerve Endings / Q000201:enzymology*; D009475:Neurons, Afferent / Q000201:enzymology*; D010625:Phenylethanolamine N-Methyltransferase / Q000378:metabolism*; D012154:Reticular Formation / Q000201:enzymology; D012701:Serotonin / Q000378:metabolism; D013378:Substantia Nigra / Q000166:cytology / Q000201:enzymology*; D014446:Tyrosine 3-Monooxygenase / Q000378:metabolism
chemical_list: D012701:Serotonin; D014446:Tyrosine 3-Monooxygenase; D010625:Phenylethanolamine N-Methyltransferase; D006136:GTP Cyclohydrolase; D001142:Aromatic-L-Amino-Acid Decarboxylases; D004298:Dopamine