Expression of proliferating cell nuclear antigen in Wilms' tumor.
OBJECTIVE
To investigate the expression of proliferating cell nuclear antigen (PCNA) and its clinical significance in Wilms' tumor.


METHODS
PCNA immunoreactivity on paraffin-embedded tissues was assessed retrospectively in 35 cases using labelled streptaridin biotin (LSAB) procedure, and its association with clinical and other biological features was studied.


RESULTS
Twelve tumors (34.29%) expressed PCNA. Expression rate increased with the advance of stage (P < 0.05). The S-phase fraction and proliferation index (PI) in children with PCNA positive expression were higher than those in children with negative PCNA expression (P < 0.05). The survival rate (16.67%) in patients with positive PCNA expression was significantly lower than that (73.91%) in no-expression ones (P < 0.01).


CONCLUSIONS
PCNA is a sensitive indicator for cell proliferation and is clinically useful in identifying a poor prognostic group of patients with Wilms' tumor.
Chen
F
F
Department of Pediatric Surgery, Xin Hua Hospital, Shanghai Second Medical University, China.
Liu
G
G
Zhang
Z
Z
Li
Z
Z
Cai
W
W
D002648:Child; D002675:Child, Preschool; D005260:Female; D005500:Follow-Up Studies; D006801:Humans; D007223:Infant; D008297:Male; D011379:Prognosis; D018809:Proliferating Cell Nuclear Antigen / Q000378:metabolism*; D012189:Retrospective Studies; D009396:Wilms Tumor / Q000378:metabolism / Q000473:pathology*
D018809:Proliferating Cell Nuclear Antigen