Cloning of a breakpoint cluster region on chromosome 14 in uterine leiomyoma.
A recurrent reciprocal chromosomal translocation, t(12;14)(q15;q24) is frequently observed in uterine leiomyoma. Chromosome 12 breakpoints have been shown to occur in a region of approximately 150 kb that contains the gene for a high mobility group protein (HMGI-C). The breakpoint region on chromosome 14 has not been precisely defined. We have generated a contig of overlapping yeast artificial chromosome (YAC) clones approximately 3 Mb in size. Fluorescence in situ hybridization (FISH) analysis showed that this contig spanned the t(12;14) breakpoints in three uterine leiomyomas and that the breakpoints in these tumors occurred within a 1 Mb region. A 30 kb cosmid spanning one of the breakpoints was isolated to set the stage for identifying regions on chromosome 14 that may cause this region to be a preferential site for chromosomal translocation.
Bhugra
B
B
Department of Molecular Genetics, Biochemistry, and Microbiology, University of Cincinnati College of Medicine, OH 45267-0524, USA.
Smolarek
T A
TA
Lynch
R A
RA
Meloni
A M
AM
Sandberg
A A
AA
Deaven
L
L
Menon
A G
AG
D018244:Chromosomes, Artificial, Yeast / Q000235:genetics; D002881:Chromosomes, Human, Pair 12 / Q000235:genetics; D002883:Chromosomes, Human, Pair 14 / Q000235:genetics*; D003001:Cloning, Molecular; D004273:DNA, Neoplasm / Q000235:genetics*; D005260:Female; D005819:Genetic Markers; D006801:Humans; D007621:Karyotyping; D007889:Leiomyoma / Q000235:genetics*; D016133:Polymerase Chain Reaction; D014178:Translocation, Genetic / Q000235:genetics*; D014594:Uterine Neoplasms / Q000235:genetics*
D004273:DNA, Neoplasm; D005819:Genetic Markers