Structural analogues of TaqMan probes for real-time quantitative PCR.
We have prepared and evaluated a series of structural analogues of TaqMan PCR probes in an effort to identify second-generation probes with improved physical properties and performance. Modifications have included non-nucleosidic dye linkers, 2'-O-Me RNA substitutions, and pyrimidine C5-propyne substitutions.
Kuimelis
R G
RG
PE Applied Biosystems, Foster City, CA 94404, USA.
Livak
K J
KJ
Mullah
B
B
Andrus
A
A
D001483:Base Sequence; D017931:DNA Primers / Q000737:chemistry*; D007202:Indicators and Reagents; D015336:Molecular Probe Techniques; D015345:Oligonucleotide Probes / Q000737:chemistry*; D043264:Phosphodiesterase I; D010727:Phosphoric Diester Hydrolases; D016133:Polymerase Chain Reaction / Q000379:methods*; D012313:RNA / Q000737:chemistry; D019914:Taq Polymerase; D013997:Time Factors
D017931:DNA Primers; D007202:Indicators and Reagents; D015345:Oligonucleotide Probes; D012313:RNA; D019914:Taq Polymerase; D010727:Phosphoric Diester Hydrolases; D043264:Phosphodiesterase I