Expression of GCF protein is dependent on the cell cycle.
GCF is reported to be a transcriptional regulator that binds to specific GC-rich sequences in the epidermal growth factor (EGF) receptor gene promotor and to represses its transcription. In this study, to examine the expression of GCF in cell cycle, we have developed antibodies against GCF protein. The anti-GCF antibody recognized a major band with a molecular weight of approximately 100 kda present in human Hela cell line by immunoprecipitation followed by Western blotting. Although GCF proteins were detected throughout the cell cycle, it reached the highest level in G1 phase, and fell down to the lowest level during G2/M phase. Thus, the expression of GCF is regulated in a cell cycle-dependent manner, suggesting a potential role of GCF in the control of cell growth.
Mao
P
P
Laboratory of Gene Regulation, Cancer Institute, Hokkaido University School of Medicine, Japan.
Takimoto
M
M
Wei
G
G
Sakai
N
N
Senmaru
N
N
Fujita
H
H
Kuzumaki
N
N
D001665:Binding Sites; D015153:Blotting, Western; D002453:Cell Cycle; D066246:ErbB Receptors / Q000235:genetics; D005786:Gene Expression Regulation; D006367:HeLa Cells; D006801:Humans; D008970:Molecular Weight; D011401:Promoter Regions, Genetic; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis / Q000302:isolation & purification; D012097:Repressor Proteins / Q000096:biosynthesis* / Q000302:isolation & purification; D014158:Transcription, Genetic
C072278:GCFC2 protein, human; D011993:Recombinant Fusion Proteins; D012097:Repressor Proteins; D066246:ErbB Receptors