[Assay of anti-corneal endothelial antibody after penetrating keratoplasty].
OBJECTIVE
To study the effective materials in the immune response and the humoral immunity in graft rejective reaction in the host after penetrating keratoplasty (PKP).


METHODS
PK was performed on 2 groups of monkeys with a 5.5 mm button in a 5.0 mm bed. The heterogroup (human to monkey) had 4 monkeys, and homo-group (monkey to monkey) also 4. Nelken's method was modified. Two kinds of antigens, corneal endothelial and stromal antigens, had been made the first time. Enzyme linked immunosorbent assay (ELISA) was used to detect the anti-corneal endothelial antibody (ACEAb) in the serum and aqueous humor of the host.


RESULTS
After PKP, the anti-corneal stromal antibody did not increase obviously, while the increase of ACEAb was distinct.


CONCLUSIONS
Humoral immune response can occur in the host after PK, and the effective material is ACEAb.
Tang
N
N
Department of Ophthalmology, Affiliated Nanfang Hospital, First Military Medical University, Guangzhou.
Li
C
C
Xu
J
J
D000818:Animals; D000906:Antibodies / Q000378:metabolism*; D001082:Aqueous Humor / Q000378:metabolism*; D004728:Endothelium, Corneal / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D015948:Keratoplasty, Penetrating; D008253:Macaca mulatta; D008297:Male; D014183:Transplantation, Heterologous
D000906:Antibodies