Characterization of a 54-kDa heat-shock-inducible protein of Pasteurella haemolytica.
Growth-condition-dependent antigens play a role in the virulence or protective capacity of many organisms. Enhanced production of an approximately 54-kDa protein was detected in heat-shocked cultures of Pasteurella haemolytica. The heat-shock-inducible protein cross-reacted with antibodies to 60-kDa heat-shock proteins of Mycobacterium tuberculosis, Chlamydia, and Escherichia coli GroEL. A probe containing the E. coli groEL operon hybridized with fragments of P. haemolytica chromosomal DNA on Southern blots. Immunoblots of the 54-kDa protein using serum from 20 calves that were challenged experimentally with P. haemolytica resulted in band densities that were significantly different between calves with high and low lesion scores. Results of the study suggest that the 54-kDa heat-shock protein may be a growth-condition-dependent immunogen that is one component of resistance to pneumonic pasteurellosis.
Mosier
D
D
Department of Diagnostic Medicine/Pathobiology, College of Veterinary Medicine, Kansas State University, Manhattan 66506, USA. mosier_d@vet.ksu.edu
Iandolo
J
J
Rogers
D
D
Uhlich
G
G
Crupper
S
S
D000818:Animals; D000906:Antibodies; D001426:Bacterial Proteins / Q000032:analysis / Q000096:biosynthesis* / Q000276:immunology; D002417:Cattle; D002418:Cattle Diseases; D018834:Chaperonin 60 / Q000096:biosynthesis / Q000235:genetics / Q000276:immunology; D002689:Chlamydia / Q000276:immunology; D003429:Cross Reactions; D004198:Disease Susceptibility; D004591:Electrophoresis, Polyacrylamide Gel; D004926:Escherichia coli / Q000276:immunology / Q000502:physiology; D006360:Heat-Shock Proteins / Q000032:analysis / Q000096:biosynthesis* / Q000276:immunology; D006358:Hot Temperature; D007113:Immunity, Innate; D015151:Immunoblotting; D016978:Mannheimia haemolytica / Q000276:immunology / Q000472:pathogenicity / Q000502:physiology*; D008970:Molecular Weight; D009169:Mycobacterium tuberculosis / Q000276:immunology / Q000502:physiology; D009876:Operon; D010326:Pasteurella Infections / Q000276:immunology / Q000662:veterinary; D014774:Virulence
D000906:Antibodies; D001426:Bacterial Proteins; D018834:Chaperonin 60; D006360:Heat-Shock Proteins