title: [Endonucleolysis of chromatin in rat thymus and spleen lymphocytes after irradiation].
abstract: The level of soluble DNA fragments (polydesoxynucleotides) in thymus and spleen lymphocytes is enhanced 12 h after the whole-body X-ray irradiation in dose of 0.5 and 1 Gy. The level of Ca2+, Mg(2+)-DNAase activity in lysed cells extracts, measured with calf spleen DNA, was enhanced in the region of 0.1-0.5 mM CaCl2 in incubation medium and decreased at 5 mM CaCl2. Monitoring of DNA fragmentation by electrophoresis in agarose gels showed the presence of fragments with approximately 200, 400, 800 bp length. X-ray induced DNA fragmentation is supposed to be associated with Ca2+, Mg(2+)-endonuclease activation.
authors.affiliation: Taras Shevchenko State University, Kyiv, Ukraine.
authors.forename: V O
authors.initials: VO
authors.lastname: Solodushko
authors.forename: O P
authors.initials: OP
authors.lastname: Matyshevs'ka
authors.forename: N Ie
authors.initials: NIe
authors.lastname: Kucherenko
mesh_terms: D000818:Animals; D002843:Chromatin / Q000737:chemistry / Q000528:radiation effects*; D004247:DNA / Q000032:analysis; D053938:DNA Fragmentation / Q000528:radiation effects; D004587:Electrophoresis, Agar Gel; D004706:Endodeoxyribonucleases / Q000378:metabolism*; D004789:Enzyme Activation / Q000528:radiation effects; D009707:Nucleosomes / Q000528:radiation effects; D051381:Rats; D017208:Rats, Wistar; D013154:Spleen / Q000166:cytology / Q000528:radiation effects*; D013601:T-Lymphocytes / Q000201:enzymology / Q000528:radiation effects*; D013950:Thymus Gland / Q000166:cytology / Q000528:radiation effects*; D014916:Whole-Body Irradiation
chemical_list: D002843:Chromatin; D009707:Nucleosomes; D004247:DNA; D004706:Endodeoxyribonucleases; C029367:calcium magnesium dependent endodeoxyribonuclease
vernacular_title: Endonukleoliz khromatynu u limfotsytakh selezinky ta tymusa shchuriv pislia oprominennia.