[Detection of M. tuberculosis streptomycin-resistant gene].
OBJECTIVE
To observe the mutations of rpsL gene in M. tuberculosis streptomycin-resistant isolates, and to develop a new method for detecting drug resistance.


METHODS
Detecting the rpsL genes with PCR-SSCP and analyzing their codons 43 by PCR-RFLP.


RESULTS
Strain H37Rv was used as a control. In 22 M. tuberculosis clinical isolates, the rpsL PCR fragments from 5 drug-susceptible isolates had no differences in the SSCP profiles with strain H37Rv, and were restricted by Mbo II. 11 of the 13 streptomycin-resistant isolates showed apparent differences in the SSCP profiles and were not digested with Mbo II. 1 of the 4 other drug-resistant isolates also had apparent SSCP differences and was not digested by Mbo II.


CONCLUSIONS
The results suggested that the rpsL gene mutation was frequently observed in M. tuberculosis streptomycin-resistant isolates, and usually situated at codon 43, PCR-SSCP and PCR-RFLP might become a simple, rapid and reliable diagnostic test for drug resistance.
Wu
X
X
Tuberculosis Research Laboratory, 309th Hospital, PLA, Beijing.
Zhuang
Y
Y
He
X
X
D000904:Antibiotics, Antitubercular / Q000494:pharmacology; D003062:Codon; D004352:Drug Resistance, Microbial / Q000235:genetics; D005798:Genes, Bacterial; D006801:Humans; D009169:Mycobacterium tuberculosis / Q000235:genetics* / Q000302:isolation & purification; D017354:Point Mutation; D016133:Polymerase Chain Reaction; D018807:Polymorphism, Single-Stranded Conformational; D012269:Ribosomal Proteins / Q000235:genetics*; D013307:Streptomycin / Q000494:pharmacology; D014397:Tuberculosis, Pulmonary / Q000382:microbiology
D000904:Antibiotics, Antitubercular; D003062:Codon; D012269:Ribosomal Proteins; C034212:ribosomal protein S12; D013307:Streptomycin