title: New intron-containing human tRNA(Leu) genes.
abstract: Three new human nuclear tRNA(Leu) genes have been isolated and sequenced using the PCR technique. Two of them represent genes containing a CAA anticodon and both contain introns of 22 nucleotides in length but differing in sequence. Intron-containing prolongated anticodon stems can be folded into a secondary structure similar to that of yeast pre-tRNA(Leu). The evolutionary conserved secondary structure suggests the same role of intron sequences in the human and yeast pre-tRNA(Leu) maturation pathway.
authors.affiliation: Department of Gene Expression, Adam Mickiewicz University in Poznań, Poland.
authors.forename: U
authors.initials: U
authors.lastname: Karwowska
authors.forename: Z
authors.initials: Z
authors.lastname: Szweykowska-Kulińska
mesh_terms: D000926:Anticodon / Q000235:genetics; D001483:Base Sequence; D017931:DNA Primers / Q000235:genetics; D019143:Evolution, Molecular; D006801:Humans; D007438:Introns; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation; D016133:Polymerase Chain Reaction; D012322:RNA Precursors / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D012323:RNA Processing, Post-Transcriptional; D012331:RNA, Fungal / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D012356:RNA, Transfer, Leu / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D012689:Sequence Homology, Nucleic Acid
chemical_list: D000926:Anticodon; D017931:DNA Primers; D012322:RNA Precursors; D012331:RNA, Fungal; D012356:RNA, Transfer, Leu