Sequencing DNA using mass spectrometry for ladder detection.
Sequencing of DNA fragments of 130 and 200 bp using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry for DNA ladder detection was demonstrated. With further improvement in mass resolution and detection sensitivity, mass spectrometry shows great promise for routine DNA sequencing in the future.
Taranenko
N I
NI
Life Science Division, Oak Ridge National Laboratory, Oak Ridge, TN 37831-6378, USA andYale University Medical Center, New Haven, CT, USA.
Allman
S L
SL
Golovlev
V V
VV
Taranenko
N V
NV
Isola
N R
NR
Chen
C H
CH
D010582:Bacteriophage lambda / Q000235:genetics; D004247:DNA / Q000737:chemistry / Q000235:genetics; D004279:DNA, Viral / Q000737:chemistry / Q000235:genetics; D008970:Molecular Weight; D012680:Sensitivity and Specificity; D017422:Sequence Analysis, DNA / Q000379:methods*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods*
D004279:DNA, Viral; D004247:DNA