[Diagnosis of mycotic infections in oncology patients using the polymerase chain reaction].
Diagnosis of mycotic infections is despite the immense effort devoted to this problem still very inaccurate. A new and promising method is the polymerase chain reaction, PCR, which theoretically can detect a single cell. In their original study the authors decided to develop a new method for the detection of fungi by PCR and to compare this examination with post-mortem findings. Thus it was possible to determine sufficiently reliably the sensitivity and specificity of the method. For the detection of fungi the authors selected the sequence coding for a small subunit of ribosomal RNA (18S rDNA). The method is able to detect the amount of DNA from some 10-100 cells. The sensitivity was 90% and the specificity 92%. The method is so far too laborious for common practice, its simplification would be however very useful.
Vorlícek
J
J
II. interní klinika FN, Brno-Bohunice.
Mayer
J
J
Kovarík
A
A
Cíhalová
J
J
Kubálek
V
V
Dvoráková
D
D
D000328:Adult; D000368:Aged; D004271:DNA, Fungal / Q000032:analysis; D006801:Humans; D008875:Middle Aged; D009181:Mycoses / Q000150:complications / Q000175:diagnosis*; D009369:Neoplasms / Q000150:complications*; D009894:Opportunistic Infections / Q000150:complications / Q000175:diagnosis*; D016133:Polymerase Chain Reaction
D004271:DNA, Fungal
Diagnostika mykotických infekcí u onkologicky nemocných pomocí polymerázové retĕzové reakce.