title: Processing of vertebrate box C/D small nucleolar RNAs in plant cells.
abstract: The recent isolation of a number of plant box C/D small nucleolar (sno)RNAs demonstrates the conservation in plants of sequence and structural elements of processed box C/D snoRNAs. Boxes C and D, and terminal inverted repeats are known to be essential for accumulation and processing in vertebrates and yeast. Processing of vertebrate box C/D snoRNAs was examined by expression of various mouse hsc70 intron 5-U14 constructs in tobacco protoplasts. Full-length U14 and internally deleted U14 accumulated in the plant cells. Human U3 and U8 fragments, consistent with processing to internal box C/C' sequences, also accumulated in the plant cells. The similarity of processing behaviour of the vertebrate box C/D constructs in tobacco protoplasts and Xenopus oocytes suggests the mechanism of processing, involving recognition and association of proteins, is conserved in plants.
authors.affiliation: Department of Cell and Molecular Genetics, Scottish Crop Research Institute, Dundee, UK.
authors.forename: D J
authors.initials: DJ
authors.lastname: Leader
authors.forename: G P
authors.initials: GP
authors.lastname: Clark
authors.forename: J
authors.initials: J
authors.lastname: Boag
authors.forename: J A
authors.initials: JA
authors.lastname: Watters
authors.forename: C G
authors.initials: CG
authors.lastname: Simpson
authors.forename: N J
authors.initials: NJ
authors.lastname: Watkins
authors.forename: E S
authors.initials: ES
authors.lastname: Maxwell
authors.forename: J W
authors.initials: JW
authors.lastname: Brown
mesh_terms: D000818:Animals; D001483:Base Sequence; D005260:Female; D006801:Humans; D066298:In Vitro Techniques; D007438:Introns; D051379:Mice; D009838:Oligodeoxyribonucleotides / Q000235:genetics; D009865:Oocytes / Q000378:metabolism; D030821:Plants, Genetically Modified; D010947:Plants, Toxic; D012323:RNA Processing, Post-Transcriptional; D012326:RNA Splicing; D012342:RNA, Small Nuclear / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D014026:Nicotiana / Q000235:genetics / Q000378:metabolism; D014981:Xenopus
chemical_list: D009838:Oligodeoxyribonucleotides; D012342:RNA, Small Nuclear