A spectrometric method for determining the capability of intermolecular interaction: mono- and dideoxynucleotides.
A method for detecting the dimer of mono- and oligodeoxynucleotides is presented by using fast atom bombardment mass spectrometry (FAB-MS). The mass spectrum of a mononucleotide dpC showed an intense peak of the dimeric ion dpC:::dpC, compared with those of dpA, dpG and dpT. The mass spectrum of an equimolar mixture of dpC and dpG showed the highest peak of the dimeric ion dpC:::dpG, compared with all other combinations. The dimeric ion peaks in the mass spectra of dinucleotides d(ApT) and d(CpG) were relatively high and it may reflect the base-pairing energy due to hydrogen bonding. The degradation patterns of each dimers for d(ApT) and d(CpG) obtained by using the collision-induced dissociation (CID) technique which reflected the strength of each base-pairing.
Takayama
M
M
Pharmaceutical Sciences, Toho University, Funabashi, Japan.
Shida
T
T
Tsugita
A
A
D001482:Base Composition; D003854:Deoxyribonucleotides / Q000737:chemistry*; D015226:Dinucleoside Phosphates / Q000138:chemical synthesis / Q000737:chemistry*; D006860:Hydrogen Bonding; D016339:Spectrometry, Mass, Fast Atom Bombardment / Q000379:methods*; D013329:Structure-Activity Relationship
D003854:Deoxyribonucleotides; D015226:Dinucleoside Phosphates