An improved method for chemical deglycosylation of gastric mucus glycoprotein.
We describe an improved method for chemical deglycosylation of gastric mucin which involves: reduction, alkylation, desialylation, periodate oxidation with beta-elimination and two-steps of TFMSA/anisole treatment. The product was 96% deglycosylated protein with amino acid composition similar to purified mucin with apparent molecular weight of 90 kDa.
Paszkiewicz-Gadek
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Department of General and Organic Chemistry, Medical Academy of Białystok.
Gindzieński
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Porowska
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D000468:Alkalies / Q000737:chemistry; D000478:Alkylation; D000596:Amino Acids / Q000032:analysis; D000818:Animals; D000840:Anisoles / Q000737:chemistry; D002623:Chemistry Techniques, Analytical; D005617:Chlorofluorocarbons, Methane / Q000737:chemistry; D002849:Chromatography, Gas; D005752:Gastric Mucins / Q000032:analysis / Q000737:chemistry / Q000302:isolation & purification*; D005753:Gastric Mucosa / Q000378:metabolism*; D006023:Glycoproteins / Q000032:analysis / Q000737:chemistry / Q000302:isolation & purification*; D006868:Hydrolysis; D008970:Molecular Weight; D009093:Mucus / Q000737:chemistry*; D010084:Oxidation-Reduction; D011108:Polymers / Q000737:chemistry; D013451:Sulfonic Acids / Q000737:chemistry; D013552:Swine
D000468:Alkalies; D000596:Amino Acids; D000840:Anisoles; D005617:Chlorofluorocarbons, Methane; D005752:Gastric Mucins; D006023:Glycoproteins; D011108:Polymers; D013451:Sulfonic Acids; C060998:anisole; C009554:fluoroform