A study of apolipoprotein A-IV genetic polymorphism, serum lipids and lipoproteins in Beijing habitants.
OBJECTIVE
Apolipoprotein (apo) A-IV genetic polymorphism and its effect on serum lipids, apoA-I and apoA-IV were investigated in order to clarify the role of apoA-IV gene during the development of hyperlipidemia.


METHODS
Four polymorphic sites (codon 127, 167, 347 and 360) of apoA-IV gene (exon 3) were determined in two groups of inhabitants in Beijing (Group I: 145 healthy individuals; Group II: 41 cases of hyperlipidemic patients and controls) by PCR-RFLP technique. Serum concentrations of HDL-cholesterol (HDL-C), HDL3-C, HDL2-C, triglyceride (TG), total cholesterol (TC), apoA-I and apoA-IV were studied among the individuals with different genotypes of apoA-IV.


RESULTS
In Group I, frequencies of the alleles were 0.648 and 0.352 in codon 127; 0.972 and 0.028 in codon 167; 0.817 and 0.183 in codon 347. Two common alleles were 0.941 and 0.059 in codon 360. The results indicated that cases of codon 127 heterozygotes had a significantly higher serum TC level and cases of apoA-IV Ser127 homozygotes kept a markedly low TG level. Both homozygotes and heterozygotes which carried apoA-IV His 360 exhibited a significantly higher concentration of TC in comparing with that of apoA-IV Gln 360 homozygotes. The data from Group II showed that the allele frequency of His 360 had a significant difference between patients and controls.


CONCLUSIONS
Certain polymorphic sites of apoA-IV gene might influence the serum lipid levels in both healthy persons and hyperlipidemic patients. His 360 polymorphic position might have a relationship with the development of hyperlipidemia.
Wang
Z
Z
Department of Pathology, Peking Union Medical College, Beijing, China.
She
M
M
Xu
M
M
Wang
Z
Z
Xia
R
R
D000328:Adult; D000483:Alleles; D001054:Apolipoproteins A / Q000097:blood* / Q000235:genetics*; D002681:China; D002784:Cholesterol / Q000097:blood*; D005260:Female; D006801:Humans; D006949:Hyperlipidemias / Q000097:blood; D008055:Lipids / Q000097:blood*; D008297:Male; D008875:Middle Aged; D011110:Polymorphism, Genetic
D001054:Apolipoproteins A; D008055:Lipids; C022205:apolipoprotein A-IV; D002784:Cholesterol