Re-examination of 5' end of cDNA of transcription factor GCF.
N-terminal region of transcriptional factor GCF was re-examined as the published amino acid sequence is extremely basic. 5'Rapid Amplification of cDNA End (5' RACE) analysis was performed and the result revealed that sequence of the first 308 bases of published GCF cDNA does not conform to the one obtained by 5' RACE. Sequence of genomic GCF DNA is consistent with the new cDNA sequence but not with the published one. Results of ordinal RT-PCR analyses are consistent with that of 5' RACE.
Takimoto
M
M
Laboratory of Gene Regulation, Cancer Institute, Hokkaido University School of Medicine, Japan.
Mao
P
P
Wei
G
G
Johnson
A C
AC
Kuzumaki
N
N
D001483:Base Sequence; D018076:DNA, Complementary / Q000737:chemistry*; D006367:HeLa Cells; D006801:Humans; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000737:chemistry; D012097:Repressor Proteins / Q000096:biosynthesis / Q000235:genetics*; D014407:Tumor Cells, Cultured
D018076:DNA, Complementary; C072278:GCFC2 protein, human; D011994:Recombinant Proteins; D012097:Repressor Proteins