title: The effect of recombinant recoverin on the photoresponse of truncated rod photoreceptors.
abstract: Recoverin is a heterogeneously acylated calcium-binding protein thought to regulate visual transduction. Its effect on the photoresponse was investigated by dialyzing the recombinant protein into truncated salamander rod outer segments. At high Ca2+ (Ca), myristoylated recoverin (Ca-recoverin) prolonged the recovery phase of the bright flash response but had less effect on the dim flash response. The prolongation of recovery had an apparent Kd for Ca of 13 microM and a Hill coefficient of 2. The prolongation was shown to be mediated by inhibition of rhodopsin deactivation. After a sudden imposed drop in Ca concentration, the effect of recoverin switched off with little lag. The myristoyl (C14:0) modification of recoverin increased its activity 12-fold, and the C12:0 or C14:2 acyl group gave similar effects. These experiments support the notion that recoverin mediates Ca-dependent inhibition of rhodopsin phosphorylation and thereby controls light-triggered phosphodiesterase activity, particularly at high light levels.
authors.affiliation: Department of Neurobiology, Stanford University School of Medicine, Stanford, CA 94305, USA.
authors.forename: M A
authors.initials: MA
authors.lastname: Erickson
authors.forename: L
authors.initials: L
authors.lastname: Lagnado
authors.forename: S
authors.initials: S
authors.lastname: Zozulya
authors.forename: T A
authors.initials: TA
authors.lastname: Neubert
authors.forename: L
authors.initials: L
authors.lastname: Stryer
authors.forename: D A
authors.initials: DA
authors.lastname: Baylor
mesh_terms: D000557:Ambystoma; D000818:Animals; D002118:Calcium / Q000502:physiology*; D002135:Calcium-Binding Proteins / Q000494:pharmacology*; D005136:Eye Proteins; D051597:Hippocalcin; D008027:Light; D008074:Lipoproteins; D009419:Nerve Tissue Proteins; D011994:Recombinant Proteins / Q000494:pharmacology*; D051603:Recoverin; D017948:Retinal Rod Photoreceptor Cells / Q000187:drug effects* / Q000502:physiology*
chemical_list: D002135:Calcium-Binding Proteins; D005136:Eye Proteins; D008074:Lipoproteins; D009419:Nerve Tissue Proteins; D011994:Recombinant Proteins; D051603:Recoverin; D051597:Hippocalcin; D002118:Calcium