[Comparison of local antigen-specific IgA responses with serum IgA response after intrapulmonary antigen immunization in healthy subjects].
OBJECTIVE
To observe local lung and serum antigen-specific IgA response after local lung antigen immunization (LLI).


METHODS
11 healthy subjects were immunized with keyhole limpet hemocyanine (KLH) through bronchoscopy. 10-14 days after immunization, IgA responses were obversed in bronchoalveolar lavage fluids (BALF) and serum.


RESULTS
LLI induced anti-KLH IgA response in immunized BALF and serum, and both were polymeric IgA. Anti-KLH IgA2/IgA1 ratios were greater in immunized BALF than in serum. Anti-KLH IgA activity in immunized BALF was associated with secretory component (SC), but in serum was not correlated with SC.


CONCLUSIONS
LLI could induce local antigen-specific IgA production. There were markedly differences between the structure and subclass distribution of antigen-specific IgA in immunized BALF and serum.
Shen
H
H
Zhejiang Medical University Affiliated Second Hospital, Hangzhou.
Hua
Y
Y
Liu
F
F
D000276:Adjuvants, Immunologic; D000328:Adult; D001704:Biopolymers / Q000378:metabolism; D001992:Bronchoalveolar Lavage Fluid / Q000276:immunology; D005260:Female; D006433:Hemocyanins / Q000276:immunology; D006801:Humans; D007070:Immunoglobulin A / Q000145:classification / Q000276:immunology / Q000378:metabolism*; D008168:Lung / Q000276:immunology*; D008297:Male
D000276:Adjuvants, Immunologic; D001704:Biopolymers; D007070:Immunoglobulin A; C034639:polymeric IgA; D006433:Hemocyanins; C032808:keyhole-limpet hemocyanin