Mammalian tRNA(Lys)3 and pre-tRNA(Lys)3 variants as primers and inhibitors of viral cDNA synthesis by HIV reverse transcriptase in vitro.
We constructed a set of mammalian tRNA(Lys)3-pseudogenes, whose pre-tRNAs are processing-deficient in HeLa extract. The 3'-flanking region was designed partially or completely complementary to the PBS-flanking nucleotides of the U5-region of HIV-1 genomic RNA. We show that only the pre-tRNA with completely hybridized 3'-flanking region is efficiently extended by the HIV-1 reverse transcriptase, whereas the partially complementary pre-tRNA(Lys)3, the 3'-terminal 10 nucleotides of which are not hybridized to the viral template, is unable to prime the cDNA-synthesis.
Schäfer
S
S
Institut für Biochemie, Bayerische Julius-Maximilians-Universität, Würzburg, Germany.
Heumann
H
H
Gross
H J
HJ
D000818:Animals; D019380:Anti-HIV Agents / Q000494:pharmacology*; D001483:Base Sequence; D017931:DNA Primers; D006367:HeLa Cells; D006801:Humans; D008322:Mammals; D009690:Nucleic Acid Conformation; D009843:Oligoribonucleotides / Q000737:chemistry / Q000494:pharmacology; D011544:Pseudogenes; D012346:RNA, Transfer, Amino Acyl / Q000096:biosynthesis / Q000737:chemistry / Q000235:genetics*; D013698:Templates, Genetic; D014158:Transcription, Genetic
D019380:Anti-HIV Agents; D017931:DNA Primers; D009843:Oligoribonucleotides; D012346:RNA, Transfer, Amino Acyl