Conformational change of DNA on the formation of DNA-anti-DNA antibody immune complex.
Monoclonal antibodies were purified from the culture medium of hybridoma from systemic lupus erythematosus (SLE)-prone MRL/Mp-Ipr-Ipr mice with affinity chromatography. Binding activity of the antibodies to double-stranded DNA from salmon milt was actually shown in enzyme-linked immunosorbent assay (ELISA). Circular dichroism (CD) spectrum of DNA solution (150 mM NaCl, 20 mM Na2HPO4, pH 7.4) which showed B-type conformation, changed significantly by addition of the anti-DNA antibodies. These results indicate that conformational change of DNA occurred by binding of anti-DNA antibodies, forming DNA-anti-DNA antibody immune complex.
Kitamura
H
H
Division of Bioscience, Graduate School of Environmental Earth Science, Hokkaido University, Sapporo, Japan.
Shindo
K
K
Sasabe
M
M
Matsuura
E
E
Sakairi
N
N
Nishi
N
N
D000818:Animals; D000911:Antibodies, Monoclonal; D000936:Antigen-Antibody Complex; D002942:Circular Dichroism; D004247:DNA / Q000737:chemistry* / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay; D006825:Hybridomas; D008180:Lupus Erythematosus, Systemic / Q000235:genetics*; D051379:Mice; D008817:Mice, Mutant Strains; D009690:Nucleic Acid Conformation
D000911:Antibodies, Monoclonal; D000936:Antigen-Antibody Complex; D004247:DNA