title: The effect of latent adenovirus 5 infection on cigarette smoke-induced lung inflammation.
abstract: The aim of this study was to test the hypothesis that latent adenovirus (Ad) 5 infection increases the lung inflammation that follows a single acute exposure to cigarette smoke. A recently developed model of latent adenoviral infection in guinea-pigs was used. Twelve animals were infected with Ad5 (10(8) plaque-forming units) and 12 animals were sham-infected. Thirty five days later six Ad5-infected and six sham-infected animals were exposed to the smoke from five cigarettes. The remaining animals were used as controls for both infection and smoking. As markers of inflammation, the volume fraction of macrophages, T-lymphocytes, neutrophils and eosinophils were measured by quantitative histology. We found that latent Ad5-infection alone, doubled the number of macrophages in the lung parenchyma and that smoking alone, doubled the volume fraction of neutrophils in the airway wall and the volume fraction of macrophages in the lung parenchyma. Neither viral infection nor smoking, alone, had an effect on T-lymphocytes or eosinophils. However, the combination of viral infection and smoking doubled the T-lymphocyte helper cells and quadrupled the volume fraction of macrophages in the lung parenchyma. We conclude that in guinea-pigs, latent adenovirus 5 infection increases the inflammation that follows a single acute exposure to cigarette smoke, by increasing the volume fraction of macrophages and T-lymphocyte helper cells.
authors.affiliation: The University of British Columbia Pulmonary Research Laboratory, St. Paul's Hospital, Vancouver, Canada.
authors.forename: T Z
authors.initials: TZ
authors.lastname: Vitalis
authors.forename: I
authors.initials: I
authors.lastname: Kern
authors.forename: A
authors.initials: A
authors.lastname: Croome
authors.forename: H
authors.initials: H
authors.lastname: Behzad
authors.forename: S
authors.initials: S
authors.lastname: Hayashi
authors.forename: J C
authors.initials: JC
authors.lastname: Hogg
mesh_terms: D000257:Adenoviridae Infections / Q000150:complications*; D017500:Adenovirus E1A Proteins / Q000235:genetics; D000818:Animals; D001990:Bronchiolitis, Viral / Q000150:complications*; D005260:Female; D006168:Guinea Pigs; D008168:Lung / Q000473:pathology* / Q000821:virology; D016676:Macrophages, Alveolar / Q000473:pathology; D010947:Plants, Toxic; D016133:Polymerase Chain Reaction; D012906:Smoke / Q000009:adverse effects*; D016176:T-Lymphocyte Subsets / Q000473:pathology; D014026:Nicotiana
chemical_list: D017500:Adenovirus E1A Proteins; D012906:Smoke