Three-dimensional models of estrogen receptor ligand binding domain complexes, based on related crystal structures and mutational and structure-activity relationship data.
On the basis of the recently determined crystal structures of the ligand binding domains (LBDs) of the retinoic acid nuclear receptors (NRs), we present a three-dimensional (3D) molecular model of the human estrogen receptor alpha (hERalpha) LBD. A literature search for mutants affecting the binding properties has been performed; 45 out of 48 published mutants can be explained satisfactorily on the basis of the model. Estradiol has been docked into the binding pocket to probe its interactions with the protein. Energy minimizations and molecular dynamics calculations were performed for various ligand orientations. To evaluate their quality, the different models were scored using known structure-activity relationship (SAR) data for selected close estradiol homologues. The two best models explain largely the binding affinities of more distantly related ligands.
Wurtz
J M
JM
Laboratoire de Biologie Structurale, IGBMC, 1, rue Laurent Fries, BP 163, 67404 Illkirch, France.
Egner
U
U
Heinrich
N
N
Moras
D
D
Mueller-Fahrnow
A
A
D000595:Amino Acid Sequence; D018360:Crystallography, X-Ray; D004958:Estradiol / Q000031:analogs & derivatives / Q000737:chemistry / Q000378:metabolism; D047628:Estrogen Receptor alpha; D006801:Humans; D008024:Ligands; D008958:Models, Molecular; D008969:Molecular Sequence Data; D009154:Mutation; D011487:Protein Conformation; D011960:Receptors, Estrogen / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D018168:Receptors, Retinoic Acid / Q000737:chemistry / Q000378:metabolism; D013329:Structure-Activity Relationship
D047628:Estrogen Receptor alpha; D008024:Ligands; D011960:Receptors, Estrogen; D018168:Receptors, Retinoic Acid; D004958:Estradiol