title: Mechanism of resistance to amikacin and kanamycin in Mycobacterium tuberculosis.
abstract: An A1400G mutation of the rrs gene was identified in Mycobacterium tuberculosis (MTB) strain ATCC 35827 and in 13 MTB clinical isolates resistant to amikacin-kanamycin (MICs, >128 microg/ml). High-level cross-resistance may result from such a mutation since MTB has a single copy of the rrs gene. Another mechanism(s) may account for high-level amikacin-kanamycin resistance in two mutants and lower levels of resistance in four clinical isolates, all lacking the A1400G mutation.
authors.affiliation: Division of Infectious Diseases, Wayne State University School of Medicine, Detroit, Michigan 48201, USA. galangaden@oncgate.roc.wayne.edu
authors.forename: G J
authors.initials: GJ
authors.lastname: Alangaden
authors.forename: B N
authors.initials: BN
authors.lastname: Kreiswirth
authors.forename: A
authors.initials: A
authors.lastname: Aouad
authors.forename: M
authors.initials: M
authors.lastname: Khetarpal
authors.forename: F R
authors.initials: FR
authors.lastname: Igno
authors.forename: S L
authors.initials: SL
authors.lastname: Moghazeh
authors.forename: E K
authors.initials: EK
authors.lastname: Manavathu
authors.forename: S A
authors.initials: SA
authors.lastname: Lerner
mesh_terms: D000583:Amikacin / Q000494:pharmacology; D000995:Antitubercular Agents / Q000494:pharmacology*; D018432:Drug Resistance, Multiple / Q000235:genetics*; D004359:Drug Therapy, Combination / Q000494:pharmacology*; D005798:Genes, Bacterial / Q000187:drug effects / Q000235:genetics*; D006801:Humans; D007612:Kanamycin / Q000494:pharmacology; D007613:Kanamycin Resistance / Q000235:genetics*; D009154:Mutation / Q000235:genetics; D009169:Mycobacterium tuberculosis / Q000187:drug effects / Q000235:genetics*
chemical_list: D000995:Antitubercular Agents; D007612:Kanamycin; D000583:Amikacin