title: Persistence of Yersinia antigens in peripheral blood cells from patients with Yersinia enterocolitica O:3 infection with or without reactive arthritis.
abstract: OBJECTIVE
  To assess the persistence of bacterial antigens in peripheral blood cells from patients with Yersinia enterocolitica O:3-triggered reactive arthritis (ReA).
  
  
  METHODS
  Peripheral blood samples were obtained from 20 patients with Y. enterocolitica O:3 infection (11 with ReA and 9 without). These samples were studied by immunochemical techniques for the presence of Yersinia antigens at the beginning of infection and up to 4 years thereafter. Synovial fluid samples from 6 of the 11 ReA patients were also studied.
  
  
  RESULTS
  The Yersinia antigens lipopolysaccharide and heat-shock protein (HSP) were detected in peripheral blood mononuclear cells and polymorphonuclear phagocytes from all patients studied at the early phase of the disease. They were also found in the synovial fluid cells of patients with Yersinia-triggered ReA. At 4 years after the onset of infection, these bacterial antigens were still detected in the peripheral blood cells of most of the ReA patients studied.
  
  
  CONCLUSIONS
  This study has, for the first time, directly demonstrated that bacterial antigens persist for a long time in patients who develop ReA after Y. enterocolitica O:3 infection. The finding of bacterial HSP in synovial fluid cells could provide a link to the pathogenesis of ReA, since T cell responses of synovial cells have been shown to be directed against that structure. A close similarity between the bacterial and host HSP might contribute to the development of the relatively common, chronic form of this complication.
authors.affiliation: National Public Health Institute, Turku, Finland.
authors.forename: K
authors.initials: K
authors.lastname: Granfors
authors.forename: R
authors.initials: R
authors.lastname: Merilahti-Palo
authors.forename: R
authors.initials: R
authors.lastname: Luukkainen
authors.forename: T
authors.initials: T
authors.lastname: Möttönen
authors.forename: R
authors.initials: R
authors.lastname: Lahesmaa
authors.forename: P
authors.initials: P
authors.lastname: Probst
authors.forename: E
authors.initials: E
authors.lastname: Märker-Hermann
authors.forename: P
authors.initials: P
authors.lastname: Toivanen
mesh_terms: D000907:Antibodies, Bacterial; D000942:Antigens, Bacterial / Q000097:blood*; D016918:Arthritis, Reactive / Q000097:blood* / Q000382:microbiology / Q000473:pathology; D004591:Electrophoresis, Polyacrylamide Gel; D019084:Fluorescent Antibody Technique, Indirect; D006360:Heat-Shock Proteins / Q000276:immunology; D006801:Humans; D015151:Immunoblotting; D008070:Lipopolysaccharides / Q000276:immunology; D009000:Monocytes / Q000382:microbiology* / Q000473:pathology; D009504:Neutrophils / Q000382:microbiology* / Q000473:pathology; D000091402:Prohibitins; D013582:Synovial Fluid / Q000166:cytology / Q000276:immunology; D015009:Yersinia Infections / Q000097:blood* / Q000382:microbiology / Q000473:pathology; D015008:Yersinia enterocolitica / Q000276:immunology*
chemical_list: D000907:Antibodies, Bacterial; D000942:Antigens, Bacterial; D006360:Heat-Shock Proteins; D008070:Lipopolysaccharides; C000717331:PHB2 protein, human; D000091402:Prohibitins