title: Prevalence of bovine viral diarrhea virus genotypes and antibody against those viral genotypes in fetal bovine serum.
abstract: One thousand lots of pooled fetal bovine serum (FBS) were tested for contamination with bovine viral diarrhea virus (BVDV) and/or for contamination with neutralizing antibody against BVDV. Noncytopathic or cytopathic BVDV was isolated from 203 lots of FBS. Analysis of the viral isolates identified 115 type 1 and 65 type 2 BVDV isolates. An additional 23 virus isolates were mixtures of > or = 2 BVDV isolates and were not classified to viral genotype. Further characterization of the type 1 viruses identified 51 subgenotype 1a and 64 subgenotype 1b BVDV isolates. Viral neutralizing antibody was detected in 113 lots of FBS. Differential viral neutralization indicated that type 1 BVDV induced the antibody detected in 48 lots of FBS and type 2 BVDV induced the antibody detected in 16 lots of FBS.
authors.affiliation: Enteric Diseases and Food Safety Research Unit, National Animal Disease Center, USDA, Agricultural Research Service, Ames, IA 50010, USA.
authors.forename: S R
authors.initials: SR
authors.lastname: Bolin
authors.forename: J F
authors.initials: JF
authors.lastname: Ridpath
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal; D000914:Antibodies, Viral / Q000097:blood*; D001912:Bovine Virus Diarrhea-Mucosal Disease / Q000821:virology*; D002417:Cattle; D017931:DNA Primers; D005312:Fetal Blood / Q000821:virology*; D005838:Genotype; D009500:Neutralization Tests; D010576:Pestivirus / Q000235:genetics* / Q000302:isolation & purification / Q000472:pathogenicity; D016133:Polymerase Chain Reaction; D012680:Sensitivity and Specificity
chemical_list: D000911:Antibodies, Monoclonal; D000914:Antibodies, Viral; D017931:DNA Primers