title: Arabidopsis bZIP protein HY5 directly interacts with light-responsive promoters in mediating light control of gene expression.
abstract: The Arabidopsis HY5 gene has been defined genetically as a positive regulator of photomorphogenesis and recently has been shown to encode a basic leucine zipper type of transcription factor. Here, we report that HY5 is constitutively nuclear localized and is involved in light regulation of transcriptional activity of the promoters containing the G-box, a well-characterized light-responsive element (LRE). In vitro DNA binding studies suggested that HY5 can bind specifically to the G-box DNA sequences but not to any of the other LREs present in the light-responsive promoters examined. High-irradiance light activation of two synthetic promoters containing either the consensus G-box alone or the G-box combined with the GATA motif (another LRE) and the native Arabidopsis ribulose bisphosphate carboxylase small subunit gene RBCS-1A promoter, which has an essential copy of the G-box, was significantly compromised in the hy5 mutant. The hy5 mutation's effect on the high-irradiance light activation of gene expression was observed in both photosynthetic and nonphotosynthetic tissues. Furthermore, the characteristic phytochrome-mediated red light- and far-red light-reversible low-fluence induction of the G-box-containing promoters was diminished specifically in hy5 plants. These results suggest that HY5 may interact directly with the G-box in the promoters of light-inducible genes to mediate light-controlled transcriptional activity.
authors.affiliation: Department of Molecular, Cellular, and Developmental Biology, Yale University, New Haven, Connecticut 06520, USA.
authors.forename: S
authors.initials: S
authors.lastname: Chattopadhyay
authors.forename: L H
authors.initials: LH
authors.lastname: Ang
authors.forename: P
authors.initials: P
authors.lastname: Puente
authors.forename: X W
authors.initials: XW
authors.lastname: Deng
authors.forename: N
authors.initials: N
authors.lastname: Wei
mesh_terms: D017360:Arabidopsis / Q000235:genetics / Q000378:metabolism* / Q000528:radiation effects; D029681:Arabidopsis Proteins; D001483:Base Sequence; D050976:Basic-Leucine Zipper Transcription Factors; D001665:Binding Sites; D002467:Cell Nucleus / Q000378:metabolism; D018744:DNA, Plant / Q000737:chemistry / Q000378:metabolism; D018506:Gene Expression Regulation, Plant / Q000528:radiation effects*; D016350:Leucine Zippers; D008027:Light; D016297:Mutagenesis, Site-Directed; D009687:Nuclear Proteins / Q000378:metabolism*; D010940:Plant Proteins / Q000378:metabolism*; D011401:Promoter Regions, Genetic; D011993:Recombinant Fusion Proteins / Q000378:metabolism; D012045:Regulatory Sequences, Nucleic Acid; D014158:Transcription, Genetic / Q000528:radiation effects
chemical_list: D029681:Arabidopsis Proteins; D050976:Basic-Leucine Zipper Transcription Factors; D018744:DNA, Plant; C109254:HY5 protein, Arabidopsis; D009687:Nuclear Proteins; D010940:Plant Proteins; D011993:Recombinant Fusion Proteins