X-ray analysis of DNA dodecamer containing 2'-deoxy-N6-methoxyadenosine.
Oxyamines have been known as a mutagen which attacks amino groups of DNA bases. It is expected that the modified adenine derivative has a tautomer which can form a stable base pair not only with thymine but also with cytosine. For establishing such tautomerization mutagenesis, we have solved a crystal structure of DNA dodecamer containing 2'-deoxy-N6-methoxyadenosine. It is shown that the N6-methoxyadenine takes a imino form to form a Watson-Crick type pairing with cytosine in the DNA duplex.
Chatake
T
T
Faculty of Bioscience and Biotechnology, Tokyo Institute of Technology, Yokohama, Japan.
Toyoda
T
T
Tsunoda
M
M
Sunami
T
T
Ono
A
A
Ueno
Y
Y
Matsuda
A
A
Takenaka
A
A
D001482:Base Composition; D001483:Base Sequence; D018360:Crystallography, X-Ray / Q000379:methods; D004247:DNA / Q000737:chemistry*; D003839:Deoxyadenosines; D008958:Models, Molecular; D009690:Nucleic Acid Conformation; D009838:Oligodeoxyribonucleotides / Q000737:chemistry*; D013237:Stereoisomerism
C113084:2'-deoxy-N(6)-methoxyadenosine; D003839:Deoxyadenosines; D009838:Oligodeoxyribonucleotides; D004247:DNA