Simplification of a locus-specific DNA typing method (Vir typing) for Streptococcus pyogenes.
We describe a simplification of a highly discriminatory molecular typing method, called Vir typing, for Streptococcus pyogenes (D. Gardiner, J. Hartas, B. Currie, J. D. Mathews, D. J. Kemp, and K. S. Sriprakash, PCR Methods Appl. 4:288-293, 1995). The procedure can be completed within a day, is reproducible, and can be applied directly to colonies growing on primary culture plates, allowing rapid establishment of strain identity in an outbreak.
Hartas
J
J
Menzies School of Health Research, Darwin, Causarina NT, Australia. jon@menzies.su.edu.au
Hibble
M
M
Sriprakash
K S
KS
D015373:Bacterial Typing Techniques; D004269:DNA, Bacterial / Q000032:analysis* / Q000235:genetics; D016133:Polymerase Chain Reaction / Q000379:methods*; D013297:Streptococcus pyogenes / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification
D004269:DNA, Bacterial