Nitric oxide-mediated cGMP production in the islands of Calleja in the rat.
cGMP-immunostaining in the islands of Calleja (ICj) in slices incubated in vitro partially co-localized with nitric oxide synthase (NOS) inside the ICj. No cGMP-immunostaining was found outside the ICj in unstimulated slices, whereas the NO-donor sodium nitroprusside (SNP) stimulated cGMP in cells and fibers bordering on the ICj. These findings show an ongoing NO synthesis in in vitro slices and suggest a relatively restricted diffusion range for endogenously synthesized NO.
de Vente
J
J
European Graduate School of Neuroscience (EURON), Maastricht University, Department of Psychiatry and Neuropsychology, Netherlands. j.devente@np.unimaas.nl
Hopkins
D A
DA
Markerink-van Ittersum
M
M
Steinbusch
H W
HW
D000818:Animals; D006152:Cyclic GMP / Q000037:antagonists & inhibitors / Q000096:biosynthesis*; D004791:Enzyme Inhibitors / Q000494:pharmacology; D006162:Guanylate Cyclase / Q000037:antagonists & inhibitors; D008297:Male; D019331:NG-Nitroarginine Methyl Ester / Q000494:pharmacology; D009569:Nitric Oxide / Q000502:physiology*; D009599:Nitroprusside / Q000494:pharmacology; D009830:Olfactory Bulb / Q000378:metabolism*; D010069:Oxadiazoles / Q000494:pharmacology; D011810:Quinoxalines / Q000494:pharmacology; D051381:Rats; D011917:Rats, Inbred Lew; D014018:Tissue Distribution
C095284:1H-(1,2,4)oxadiazolo(4,3-a)quinoxalin-1-one; D004791:Enzyme Inhibitors; D010069:Oxadiazoles; D011810:Quinoxalines; D009599:Nitroprusside; D009569:Nitric Oxide; D006162:Guanylate Cyclase; D006152:Cyclic GMP; D019331:NG-Nitroarginine Methyl Ester