Immunochemical purification and characterization of a 74.0-kDa Schistosoma mansoni antigen.
A polypeptide antigen of 74.0 kDa molecular weight was detected in the antigenic extracts of the 3 developmental stages of Schistosoma mansoni (eggs, cercariae, and adult worms) by western blotting using BRL4 monoclonal antibody (mAb) that significantly protected mice at the levels of 51.6%, 42%, and 53.8% against challenge S. mansoni infection in 3 separate experiments. This antigen was isolated and purified from crude soluble worm antigen preparation by immunoaffinity chromatography using CNBr-activated sepharose-4B beads coupled with the BRL4 mAb. The purified antigen showed a single peak when analyzed by both high-performance liquid chromatography and high-performance capillary electrophoresis. The 74-kDa antigen was characterized as a protein in nature with 56.9% hydrophilic amino acids and 43.1% hydrophobic amino acids. This antigen was detected in 93% of urine samples from infected cases with specificity of 89% among noninfected cases using an enzyme immunoassay-fast dot-enzyme-linked immunosorbent assay based on BRL4 mAb.
Attallah
A M
AM
Biotechnology Research Center, New Damietta City, Egypt.
El Masry
S A
SA
Ismail
H
H
Attia
H
H
Abdel Aziz
M
M
Shehatta
A S
AS
Tabll
A
A
Soltan
A
A
El Wassif
A
A
D000293:Adolescent; D000328:Adult; D000368:Aged; D000596:Amino Acids / Q000032:analysis; D000818:Animals; D000909:Antibodies, Helminth / Q000276:immunology; D000911:Antibodies, Monoclonal / Q000276:immunology; D000947:Antigens, Helminth / Q000737:chemistry / Q000302:isolation & purification* / Q000652:urine; D015153:Blotting, Western; D002648:Child; D002675:Child, Preschool; D002846:Chromatography, Affinity; D002851:Chromatography, High Pressure Liquid; D004591:Electrophoresis, Polyacrylamide Gel; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006801:Humans; D006825:Hybridomas; D007116:Immunization, Passive; D051379:Mice; D008807:Mice, Inbred BALB C; D008875:Middle Aged; D010449:Peptide Mapping; D012550:Schistosoma mansoni / Q000276:immunology*; D012555:Schistosomiasis mansoni / Q000276:immunology / Q000652:urine
D000596:Amino Acids; D000909:Antibodies, Helminth; D000911:Antibodies, Monoclonal; D000947:Antigens, Helminth