Lipid changes in hepatic microsomes and its relationship to P-nitrophenol glucuronidation in an experimental model of portal hypertension.
The liver is responsible for the most important metabolic pathway of non polar compounds. The aim of the present work was to study the p-nitrophenol glucuronidation and its relationship with lipidic composition of microsomal membrane in a model of hepatic portal hypertension and hepatocellular damage induced by monocrotaline. A global increment in liver microsomal phospholipids as well as changes in the phospholipid pattern (phosphatidylethanolamine and sphingomyelin increased up to 156 +/- 13 and 195 +/- 14% respectively) were detected in monocrotaline intoxicated rats when it were compared to control rats. The microsomal cholesterol content showed a decrease in monocrotaline intoxicated rats. (4.1 +/- 0.7 against 6.6 +/- 1.5 micrograms/mg of microsomal protein, in control rats). When p-nitrophenol activity was measured, Km from monocrotaline intoxicated rats was 0.137 mM, and Vmax was 2.9 nmol of p-nitrophenol/mg microsomal protein since in control group Km was 0.322 mM, and Vmax was 4.5 nmol of p-nitrophenol/mg microsomal protein. It is concluded that monocrotaline intoxicated rats showed a different behavior in the kinetics of p-nitrophenol UDP-glucuronyltransferase, as well as a different microsomal lipidic profile, when compared to control group.
Ghanem
C
C
Catedra de Fisiopatologia y Farmacologia, Facultad de Farmacia y Bioquimica, Universidad de Buenos Aires, Argentina.
Ghisolfi
C
C
Marabotto
L
L
Ouviña
G
G
Rubio
M
M
Perazzo
J
J
Lemberg
A
A
Bengochea
L
L
D000818:Animals; D002784:Cholesterol / Q000378:metabolism; D005965:Glucuronates / Q000378:metabolism*; D014453:Glucuronosyltransferase / Q000378:metabolism; D006975:Hypertension, Portal / Q000378:metabolism*; D008658:Inactivation, Metabolic / Q000502:physiology*; D050356:Lipid Metabolism; D008297:Male; D008862:Microsomes, Liver / Q000378:metabolism*; D016686:Monocrotaline / Q000633:toxicity; D009596:Nitrophenols / Q000378:metabolism*; D010743:Phospholipids / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar
D005965:Glucuronates; D009596:Nitrophenols; D010743:Phospholipids; D016686:Monocrotaline; D002784:Cholesterol; D014453:Glucuronosyltransferase; C024836:4-nitrophenol