title: Evaluation of the biocompatibility of a nonceramic hydroxyapatite.
abstract: In this study, the biocompatibility of calcium phosphate cement (CPC) was systematically investigated, which included systemic injection acute toxicity assay, cell culture cytotoxicity assay, gene mutation assay (Ames test), chromosome aberration assay (micronucleus test), DNA damage assay (unscheduled DNA synthesis test) and implant histological evaluation. The results showed that the CPC had no toxicity and all tests for mutagenicity and potential carcinogenicity of CPC extracts are negative. CPC was implanted into the femurs of rabbits under no-load condition and the histology of specimens revealed that the implant tightly joined with the surrounding bone, only a very slight histological inflammatory reaction occurred. Thus, CPC is a highly biocompatible material and seems to be safe for application in humans.
authors.affiliation: Institute of Technical Chemical Physics, East China University of Science and Technology, Shanghai, P.R. China.
authors.forename: C
authors.initials: C
authors.lastname: Liu
authors.forename: W
authors.initials: W
authors.lastname: Wang
authors.forename: W
authors.initials: W
authors.lastname: Shen
authors.forename: T
authors.initials: T
authors.lastname: Chen
authors.forename: L
authors.initials: L
authors.lastname: Hu
authors.forename: Z
authors.initials: Z
authors.lastname: Chen
mesh_terms: D000818:Animals; D001672:Biocompatible Materials / Q000633:toxicity*; D001843:Bone Cements / Q000633:toxicity*; D016466:CHO Cells; D002130:Calcium Phosphates / Q000633:toxicity*; D002478:Cells, Cultured; D006224:Cricetinae; D003412:Cricetulus; D004247:DNA / Q000096:biosynthesis / Q000187:drug effects; D017886:Durapatite / Q000633:toxicity*; D008099:Liver / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D008297:Male; D051379:Mice; D015162:Micronucleus Tests; D009152:Mutagenicity Tests; D010316:Particle Size; D011817:Rabbits; D051381:Rats; D017207:Rats, Sprague-Dawley; D012486:Salmonella typhimurium / Q000187:drug effects
chemical_list: D001672:Biocompatible Materials; D001843:Bone Cements; D002130:Calcium Phosphates; C485828:alpha-tricalcium phosphate; C485830:tetracalcium phosphate; C485838:calcium phosphate, monobasic, anhydrous; D004247:DNA; D017886:Durapatite; C020243:calcium phosphate; C485829:calcium phosphate, dibasic, anhydrous