[Experimental research and clinical application of homologous dura mater in ocular plastic surgery].
OBJECTIVE
To explore the clinical value of the dura mater implantation in ocular plastic surgeries.


METHODS
Alcohol preserved homologous dura mater was implanted into the upper fornix under the conjunctiva of rabbit eye and light, electron microscopic histological and immunological examinations were made for the implant. 38 eyes consisting of 30 eyes of blepharoptosis, 4 eyes of blepharo-coloboma, 3 eyes of hollow socket and 1 eye of teleepicanthus were treated by homologous dura mater implantation.


RESULTS
In the follow-up periods ranging from 1.5 to 2.5 years, 26 eyes in the treated eyes were cured, 9 eyes were markedly improved, 3 eyes failed. Histological findings showed that after 1 week of implantation there was cell infiltration around the implant, at 2 weeks new collagenous tissue developed at the margin of the implant and 2-3 months after transplantation, the grafts were replace by the patient's own connective tissue. The histological examination of 3 failure cases showed that the cause of the failure was due to the ischemia of homograft. The results of unidirectional mixed lymphocyte culture showed that the stimulation index before implantation in comparison with that at 96 and 120 hours after implantation had statistical significant differences, while in comparison with that at 144 hours after implantation had no such difference. Before the implantation, the stimulation index of the lymphocyte culture at 96 hours was significantly different from that at 144 hours statistically (P < 0.05). In the comparison of specific killing activity before and after implantation, there was statistical significant difference.


CONCLUSIONS
The effects of transplantation of homologous dura mater employed in ocular plastic surgeries of blepharoptosis, blepharocoloboma, teleepicanthus and hollow socket are fairly good.
Zhao
G
G
Department of Ophthalmology, Third Hospital, Beijing Medical University.
Zhou
J
J
D000293:Adolescent; D000328:Adult; D000368:Aged; D000818:Animals; D019882:Blepharoplasty; D001763:Blepharoptosis / Q000276:immunology / Q000601:surgery*; D002648:Child; D002675:Child, Preschool; D004388:Dura Mater / Q000637:transplantation; D005260:Female; D005500:Follow-Up Studies; D006801:Humans; D007959:Lymphocyte Culture Test, Mixed; D008297:Male; D008875:Middle Aged; D011817:Rabbits