Genomic structure and chromosomal location of the human TGFbeta-receptor interacting protein-1 (TRIP-1) gene to 1p34.1.
The human TRIP-1 (transforming growth factor-beta (TGBbeta)-receptor interacting protein-1) cDNA encodes a protein able to associate specifically with the type II TGFbeta receptor. It is phosphorylated on serine and threonine by this receptor kinase which makes it a strong candidate as part of the TGFbeta signal transduction pathway. We have isolated the genomic sequence of TRIP-1 and found that the complete coding region is organised into 11 exons ranging from 39 to 397 bp and spanning approximately 9 kb of genomic DNA. The 5' flanking region lacks a TATA box but is GC-rich, suggesting that it is a constitutively expressed gene which is in agreement with its wide pattern of expression. Fluorescence in situ hybridisation mapped the TRIP-1 gene to chromosome 1p34.1 whereas a pseudogene is located on chromosome 7q32.
Galli-Stauber
C
C
Department of Biology and Technology (DIBIT), San Raffaele Scientific Institute, Milan, Italy.
Raho
G
G
Rossi
D
D
Corona
D F
DF
Pirola
B
B
Bonaglia
M C
MC
Zuffardi
O
O
Sorrentino
V
V
D002878:Chromosomes, Human, Pair 1; D002897:Chromosomes, Human, Pair 7; D039621:Eukaryotic Initiation Factor-3; D005091:Exons; D005796:Genes; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D007438:Introns; D011506:Proteins / Q000235:genetics*; D011544:Pseudogenes; D015183:Restriction Mapping
D039621:Eukaryotic Initiation Factor-3; D011506:Proteins; C096013:EIF3I protein, human