A rapid immunochromatographic test (ICT) for diagnosis of Plasmodium falciparum.
A field study was conducted to assess the sensitivity and specificity of rapid immunodiagnostic test based on detection of Plasmodium falciparum histidine-rich protein-2 (PfHRP-2) in peripheral blood for diagnosis of P. falciparum infection. Evaluation in 173 patients showed that the assay was 98.59% sensitive and 97.1% specific. There was no cross-reactivity with P. vivax. The test was positive in few patients who were found to be negative by microscopy showing the presence of antigen after curative chemotherapy. The test is a valuable diagnostic tool for falciparum malaria, especially in emergency/field situations requiring rapid diagnosis.
Valecha
N
N
Malaria Research Centre, Indian Council of Medical Research, Sham Nath Marg, Delhi, India.
Sharma
V P
VP
Devi
C U
CU
D000818:Animals; D002845:Chromatography; D003429:Cross Reactions; D005500:Follow-Up Studies; D006801:Humans; D016778:Malaria, Falciparum / Q000097:blood / Q000175:diagnosis* / Q000469:parasitology*; D011506:Proteins / Q000032:analysis*; D015800:Protozoan Proteins / Q000097:blood*; D011933:Reagent Kits, Diagnostic; D012680:Sensitivity and Specificity; D000099053:Histidine-Rich Glycoprotein
D011506:Proteins; D015800:Protozoan Proteins; D011933:Reagent Kits, Diagnostic; D000099053:Histidine-Rich Glycoprotein