title: Antinociception produced by microinjection of morphine in the rat periaqueductal gray is enhanced in the foot, but not the tail, by intrathecal injection of alpha1-adrenoceptor antagonists.
abstract: Antinociception produced by microinjection of morphine in the ventrolateral periaqueductal gray is mediated in part by alpha2-adrenoceptors in the spinal cord dorsal horn. However, several recent reports demonstrate that microinjection of morphine in the ventrolateral periaqueductal gray inhibits nociceptive responses to noxious heating of the tail by activating descending neuronal systems that are different from those that inhibit the nociceptive responses to noxious heating of the feet. More specifically, alpha2-adrenoceptors appear to mediate the antinociception produced by morphine using the tail-flick test, but not that using the foot-withdrawal or hot-plate tests. The present study extended these findings and determined the role of alpha1-adrenoceptors in mediating the antinociceptive effects of morphine microinjected into the ventrolateral periaqueductal gray using both the foot-withdrawal and the tail-flick responses to noxious radiant heating in lightly anesthetized rats. Intrathecal injection of selective antagonists was used to determine whether the antinociceptive effects of morphine were modulated by alpha1-adrenoceptors. Injection of the selective alpha1-adrenoceptor antagonists prazosin or WB4101 potentiated the increase in the foot-withdrawal response latency produced by microinjection of morphine in the ventrolateral periaqueductal gray. In contrast, either prazosin or WB4101 partially reversed the increase in the tail-flick response latency produced by morphine. These results indicate that microinjection of morphine in the ventrolateral periaqueductal gray modulates nociceptive responses to noxious heating of the feet by activating descending neuronal systems that are different from those that inhibit the nociceptive responses to noxious heating of the tail. More specifically, alpha1-adrenoceptors mediate a pro-nociceptive action of morphine using the foot-withdrawal response, but in contrast, alpha1-adrenoceptors appear to mediate part of the antinociceptive effect of morphine determined using the tail-flick test.
authors.affiliation: Department of Pharmacology, The University of Illinois at Chicago, 835 S. Wolcott, Chicago, IL 60612, USA.
authors.forename: F
authors.initials: F
authors.lastname: Fang
authors.forename: H K
authors.initials: HK
authors.lastname: Proudfit
mesh_terms: D000317:Adrenergic alpha-Antagonists / Q000494:pharmacology*; D000344:Afferent Pathways / Q000187:drug effects; D000818:Animals; D001522:Behavior, Animal / Q000187:drug effects; D004146:Dioxanes / Q000494:pharmacology; D005260:Female; D005528:Foot; D019329:Idazoxan / Q000494:pharmacology*; D007278:Injections, Spinal; D008845:Microinjections; D009020:Morphine / Q000494:pharmacology*; D009294:Narcotics / Q000494:pharmacology*; D009619:Nociceptors / Q000502:physiology*; D010146:Pain / Q000503:physiopathology; D010487:Periaqueductal Gray / Q000187:drug effects / Q000502:physiology*; D011224:Prazosin / Q000494:pharmacology; D051381:Rats; D017207:Rats, Sprague-Dawley; D018340:Receptors, Adrenergic, alpha-1 / Q000502:physiology; D013116:Spinal Cord / Q000737:chemistry / Q000187:drug effects / Q000502:physiology; D013623:Tail
chemical_list: D000317:Adrenergic alpha-Antagonists; D004146:Dioxanes; D009294:Narcotics; D018340:Receptors, Adrenergic, alpha-1; D009020:Morphine; C010654:(2-(2',6'-dimethoxy)phenoxyethylamino)methylbenzo-1,4-dioxane; D011224:Prazosin; D019329:Idazoxan