title: Analysis of the T cell receptor variability of tumor-infiltrating lymphocytes in colorectal carcinomas.
abstract: While there is good clinical and experimental evidence for immunological tumor control in some tumors--malignant melanomas, for instance--the immunogenicity of colorectal carcinoma (CRC) still remains unsettled. We examined surgical specimens from 4 CRC patients for T cell clones among tumor-infiltrating lymphocytes (TIL). The growth of specific lymphocyte clones in a tumor indicates an immunological response in vivo. We used a T cell receptor V beta family-specific semiquantitative PCR with additional sequencing to examine TIL for clonal expansion. In CRC, specific T cell clones could not be demonstrated. However, we observed a predominance of V beta 9 in 3 of 4 tumors.
authors.affiliation: Abteilung für Allgemeinchirurgie mit Poliklinik, Chirurgische Universitätsklinik, Freiburg, Deutschland.
authors.forename: P K
authors.initials: PK
authors.lastname: Baier
authors.forename: S
authors.initials: S
authors.lastname: Wimmenauer
authors.forename: T
authors.initials: T
authors.lastname: Hirsch
authors.forename: B U
authors.initials: BU
authors.lastname: von Specht
authors.forename: S
authors.initials: S
authors.lastname: von Kleist
authors.forename: H
authors.initials: H
authors.lastname: Keller
authors.forename: E H
authors.initials: EH
authors.lastname: Farthmann
mesh_terms: D000230:Adenocarcinoma / Q000276:immunology*; D002999:Clone Cells / Q000737:chemistry / Q000166:cytology / Q000276:immunology; D015179:Colorectal Neoplasms / Q000276:immunology*; D005260:Female; D006801:Humans; D016246:Lymphocytes, Tumor-Infiltrating / Q000737:chemistry / Q000166:cytology* / Q000276:immunology; D008297:Male; D008875:Middle Aged; D011948:Receptors, Antigen, T-Cell / Q000276:immunology*; D017421:Sequence Analysis
chemical_list: D011948:Receptors, Antigen, T-Cell