Serologic diagnosis of ovine lentiviruses by whole virus ELISA and AGID test.
Enzyme-linked immunosorbent assay (ELISA) and agar gel immunodiffusion (AGID) are the most widely used serological tests for Maedi-Visna diagnostics. The purpose of the present study was to develop an indirect whole virus ELISA and an immunodiffusion test and compare their sensitivity. A total of 747 ovine serum specimens were analysed for antibodies against this ovine lentivirus. The number of positive results in the ELISA was 430 (57.56%). In the AGID test, a positive result was found in 380 samples (50.87%). In the group of discordant results 78 (10.4%) samples tested positive by the ELISA and negative by the AGID test and 28 sera (3.7%) were found to be positive by the AGID test and negative by the ELISA. The data in this report show the ELISA to be more sensitive than the AGID test, but accurate serological diagnostics should be based on a combination of the two tests.
Celer
V
V
Department of Microbiology and Immunology, University of Veterinary and Pharmaceutical Sciences Brno, Czech Republic.
Celer
V
V
Nĕmcová
H
H
Zanoni
R G
RG
Peterhans
E
E
D000818:Animals; D000831:Animals, Newborn; D000914:Antibodies, Viral / Q000097:blood*; D015153:Blotting, Western / Q000662:veterinary; D018153:Czech Republic; D004797:Enzyme-Linked Immunosorbent Assay / Q000662:veterinary*; D005779:Immunodiffusion / Q000662:veterinary*; D011021:Pneumonia, Progressive Interstitial, of Sheep / Q000175:diagnosis*; D015203:Reproducibility of Results; D012756:Sheep; D012757:Sheep Diseases / Q000175:diagnosis*; D014790:Visna-maedi virus / Q000276:immunology*
D000914:Antibodies, Viral