Establishment and characterization of a murine cell-line derived from malignant fibrous histiocytoma of A/Jackson mouse.
A hard mass incidentally found in the right buttock of an elderly male A/J mouse was morphologically consistent with malignant fibrous histiocytoma; storiform-pleomorphic subtype. Diced pieces or suspensions of the tumor tissue were readily transplantable subcutaneously in the flank of mice of the strain. The tumor-bearing mice died mostly of rupture of the tumor into the thorax or abdomen within 4 weeks. Pulmonary and splenic metastasis were recognized only in a mouse which survived more than 10 weeks. A new cell-line was established from the original tumor without necessary cloning and was maintained in MEM (minimum essential medium) supplemented with 10% FCS for 6 months under 22 passages. Frozen stocks were then made, from which the recovery of the cells was successful. The cell growth, requiring about 20 hours for doubling of population, was a mixture of plump or stellate histiocytic cells and fibroblastic ones, both of which contained lipid droplets and retained phagocytic activity. The cell-line was designated as murine sarcoma Sendai (MuSS). Morphological versatility of the cultured cells suggested the origin of the neoplasm from multipotential, primitive mesenchymal cells.
Watanabe
I
I
Division of Surgery, NTT Tohoku Teishin Hospital, Sendai, Japan.
Kurosawa
N
N
Nishihira
T
T
D000818:Animals; D018929:Cell Culture Techniques / Q000379:methods; D002455:Cell Division; D018219:Histiocytoma, Benign Fibrous / Q000473:pathology* / Q000648:ultrastructure; D007621:Karyotyping; D008297:Male; D051379:Mice; D008805:Mice, Inbred A; D012983:Soft Tissue Neoplasms / Q000473:pathology / Q000648:ultrastructure; D014407:Tumor Cells, Cultured / Q000473:pathology* / Q000648:ultrastructure