Analytical evaluation of an improved procedure for measuring thyrotropin.
The analytical characteristics of the AxSYM Ultrasensitive hTSH-II (Abbott Laboratories) procedure for quantitation of serum thyrotropin (TSH) concentration were evaluated. Within- and between-run imprecisions, functional sensitivity, analytical interval and relative inaccuracy with respect to the Enzymum-Test TSH (Boehringer Mannheim) were studied. In all cases, the within-run and between-run of coefficients variation were lower than 6.69% and 8.12% respectively. The measurement range was tested with serial dilutions of a serum with a high thyrotropin concentration, and the procedure was found to be linear up to at least 87.0 mIU/l. The functional sensitivity was 0.018 mIU/l. The relative inaccuracy study (Passing-Bablok non-parametric linear regression) produced the following linear equation: (AxSYM) = 1.02. (ES-700)-0.03 mIU/l, with 95% confidence intervals of a (-0.05; -0.01); b (0.98; 1.06).
González-de-la-Presa
B
B
Sección de Hormonas (Servicio de Bioquímica), Ciutat Sanitària i Universitária de Bellvitge, Barcelona, Spain.
Palacios
G
G
Bonnin
R
R
Martinez
J M
JM
Navarro
M A
MA
D006801:Humans; D007124:Immunoenzyme Techniques; D011933:Reagent Kits, Diagnostic; D013972:Thyrotropin / Q000097:blood*
D011933:Reagent Kits, Diagnostic; D013972:Thyrotropin