Molecular cloning and nucleotide sequence of the pyruvate kinase gene of an actinomycete Microbispora thermodiastatica.
The gene for the thermostable pyruvate kinase of Microbispora thermodiastatica IFO 14046, a moderate thermophilic actinomycete, was cloned in Escherichia coli. This gene consists of an open reading frame of 1422 nucleotides and encodes a protein of 474 amino acids with molecular mass of 50,805 Da. The open reading frame was confirmed as the pyruvate kinase gene by comparison with the N-terminal amino acid sequence of the purified pyruvate kinase from M. thermodiastatica.
Arai
A
A
Research and Development Division, Kikkoman Corporation, Chiba, Japan.
Masuda
S
S
Matsuyama
A
A
Murakami
S
S
Nakajima
M
M
D000191:Actinomycetaceae / Q000737:chemistry* / Q000201:enzymology / Q000235:genetics* / Q000254:growth & development; D001483:Base Sequence; D005798:Genes, Bacterial / Q000235:genetics*; D008969:Molecular Sequence Data; D011770:Pyruvate Kinase / Q000737:chemistry* / Q000235:genetics; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA
D011770:Pyruvate Kinase