Detection of Leptospira DNA in patients with aseptic meningitis by PCR.
Samples of cerebrospinal fluid from 103 patients with aseptic meningitis were tested by PCR for detection of leptospires, and the results were compared with those of the microscopic agglutination test (MAT) and an enzyme-linked immunosorbent assay for detection of immunoglobulin M (ELISA-IgM). Of these samples, 39.80% were positive by PCR and 8.74 and 3.88% were positive by MAT and ELISA-IgM, respectively.
Romero
E C
EC
Department of Medical Biology, Adolfo Lutz Institute, São Paulo, Brazil. cobo@usp.br
Billerbeck
A E
AE
Lando
V S
VS
Camargo
E D
ED
Souza
C C
CC
Yasuda
P H
PH
D000293:Adolescent; D000328:Adult; D017677:Age Distribution; D002648:Child; D002675:Child, Preschool; D004269:DNA, Bacterial / Q000032:analysis; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006801:Humans; D007223:Infant; D007919:Leptospira / Q000302:isolation & purification*; D007922:Leptospirosis / Q000134:cerebrospinal fluid* / Q000150:complications; D008297:Male; D008582:Meningitis, Aseptic / Q000134:cerebrospinal fluid* / Q000150:complications / Q000382:microbiology; D008875:Middle Aged; D016133:Polymerase Chain Reaction / Q000379:methods
D004269:DNA, Bacterial