title: Production and characterization of a monoclonal antibody able to discriminate galectin-1 from galectin-2 and galectin-3.
abstract: Antisera raised against galectin-1 exhibit crossreactivities with other galectins or related molecules. In order to overcome this problem, a monoclonal antibody to human brain galectin-1 was obtained by selecting clones without reactivity toward galectin-3. This mAb specifically bound galectin-1 of various animal origins but neither galectin-2 nor galectin-3. Western-blotting analysis of soluble human brain extracts after 2D gel electrophoresis revealed only the two most acidic isoforms of galectin-1. The ability of this mAb to bind galectin-1/asialofetuin complexes indicates that its epitope is not localized in the carbohydrate recognition domain of galectin-1. This particularity induces with efficiency its monospecificity.
authors.affiliation: Laboratoire de Biochimie et Technologie des Protéines (EA 1625), Université Paris Nord, UFR Santé, Médecine et Biologie Humaine, Bobigny, France.
authors.forename: J D
authors.initials: JD
authors.lastname: Cornillot
authors.forename: M
authors.initials: M
authors.lastname: Pontet
authors.forename: C
authors.initials: C
authors.lastname: Dupuy
authors.forename: A
authors.initials: A
authors.lastname: Chadli
authors.forename: M
authors.initials: M
authors.lastname: Caron
authors.forename: R
authors.initials: R
authors.lastname: Joubert-Caron
authors.forename: P
authors.initials: P
authors.lastname: Bourin
authors.forename: D
authors.initials: D
authors.lastname: Bladier
mesh_terms: D000595:Amino Acid Sequence; D000911:Antibodies, Monoclonal; D000918:Antibody Specificity; D000943:Antigens, Differentiation / Q000032:analysis / Q000737:chemistry* / Q000276:immunology; D001923:Brain Chemistry; D003429:Cross Reactions; D004797:Enzyme-Linked Immunosorbent Assay; D037483:Galectin 1; D037501:Galectin 2; D037502:Galectin 3; D006388:Hemagglutinins / Q000032:analysis / Q000737:chemistry* / Q000276:immunology; D006801:Humans; D037102:Lectins / Q000737:chemistry; D008969:Molecular Sequence Data; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid
chemical_list: D000911:Antibodies, Monoclonal; D000943:Antigens, Differentiation; D037483:Galectin 1; D037501:Galectin 2; D037502:Galectin 3; D006388:Hemagglutinins; D037102:Lectins