title: Cholecystokinin B-type receptor signaling is involved in human pancreatic cancer cell growth.
abstract: Cholecystokinin (CCK) is known to stimulate pancreatic cancer cell growth, but no detailed CCK receptor subtype characterization and investigation of CCK receptor-mediated cellular responses in human pancreatic cancer cells have been reported thus far. In this study, CCK binding sites were identified in human pancreatic cancer cells (MIA-PaCa-2) using radioligand binding studies. Pharmacological characterization demonstrated a single class of high-affinity CCK sites on MIA-PaCa-2 cells (326 +/- 18 pM, receptor density 16.9 +/- 2.3 fmol/mg protein). These CCK binding sites displayed a typical CCKB binding profile as shown in competition studies by using different CCK-related compounds and non-peptide CCK antagonists discriminating between CCKA and CCKB sites. CCKB receptor-connected effector systems have been characterized in MIA-PaCA-2 cells, and their involvement in CCK-8S-induced proliferative effects on MIA-PaCa-2 cells has been demonstrated.
authors.affiliation: Max Planck Gesellschaft, Research Unit Pharmacological Hemostaseology at the Friedrich Schiller University Jena, Germany. rol@leech.mpg.uni-jena.de
authors.forename: R
authors.initials: R
authors.lastname: Kaufmann
authors.forename: H
authors.initials: H
authors.lastname: Schafberg
authors.forename: C
authors.initials: C
authors.lastname: Rudroff
authors.forename: P
authors.initials: P
authors.lastname: Henklein
authors.forename: G
authors.initials: G
authors.lastname: Nowak
mesh_terms: D002118:Calcium / Q000378:metabolism; D002455:Cell Division / Q000502:physiology; D006801:Humans; D015544:Inositol 1,4,5-Trisphosphate / Q000378:metabolism; D007457:Iodine Radioisotopes; D010190:Pancreatic Neoplasms; D011493:Protein Kinase C / Q000032:analysis / Q000378:metabolism; D011869:Radioligand Assay; D043887:Receptor, Cholecystokinin B; D011949:Receptors, Cholecystokinin / Q000819:agonists / Q000502:physiology*; D015398:Signal Transduction / Q000502:physiology*; D012844:Sincalide / Q000031:analogs & derivatives / Q000494:pharmacology; D013936:Thymidine / Q000378:metabolism / Q000494:pharmacology; D014316:Tritium; D014407:Tumor Cells, Cultured / Q000737:chemistry / Q000166:cytology / Q000201:enzymology
chemical_list: C090019:8-sulfocholecystokinin octapeptide; D007457:Iodine Radioisotopes; D043887:Receptor, Cholecystokinin B; D011949:Receptors, Cholecystokinin; D014316:Tritium; D015544:Inositol 1,4,5-Trisphosphate; D011493:Protein Kinase C; D012844:Sincalide; D002118:Calcium; D013936:Thymidine