title: Growth of Cowdria ruminantium in tissue culture endothelial cell lines from wild African mammals.
abstract: Endothelial cell cultures were established from several wild African mammalian species. Long-term cultures were established from three ruminants, stable antelope (Hippotragus niger), buffalo (Syncerus caffer), and eland (Tragelaphus oryx), and from an omnivore, the bushpig (Potamochoerus porcus). Cowdria ruminanntium was isolated from plasma of clinically affected animals in these four cell lines and in bovine endothelial cells used routinely for C. ruminantium propagation. Nineteen different strains of C. ruminantium from Africa and the Caribbean region were grown and maintained in these cell lines and their growth was comparable with growth in the bovine endothelial cells. The role of sable antelope, eland, and bushpigs in the epidemiology of heartwater is unknown. However, these results extend the number of cell lines that can be used to isolate and grow C. ruminantium.
authors.affiliation: University of Florida/USAID/SADC Heartwater Research Project, Veterinary Research Laboratory, Causeway, Harare, Zimbabwe.
authors.forename: G E
authors.initials: GE
authors.lastname: Smith
authors.forename: E C
authors.initials: EC
authors.lastname: Anderson
authors.forename: M J
authors.initials: MJ
authors.lastname: Burridge
authors.forename: T F
authors.initials: TF
authors.lastname: Peter
authors.forename: S M
authors.initials: SM
authors.lastname: Mahan
mesh_terms: D000349:Africa; D000818:Animals; D000835:Animals, Wild; D000866:Antelopes; D001011:Aorta; D001188:Artiodactyla; D002020:Buffaloes; D002417:Cattle; D002455:Cell Division; D002460:Cell Line; D016995:Ehrlichia ruminantium / Q000254:growth & development*; D004730:Endothelium, Vascular / Q000166:cytology / Q000382:microbiology*; D005260:Female; D006801:Humans; D008297:Male; D011651:Pulmonary Artery; D012692:Serial Passage