In vitro evaluation of the cytotoxicity of two glass-ionomer root canal sealers.
The cytotoxicity of two glass-ionomer root canal sealers (Ketac-Endo and Endion) was tested by using an established cell line, BHK21/C13. Under aseptic conditions, the sealers were prepared according to the manufacturers' directions, and 0.1 ml of each material was placed in petri dishes. After setting for 6 h, the sealers were covered with 20 x 10(4) cells per dish. The cultures were incubated at 37 degrees C for 24 h, 48 h, and 72 h. Cytotoxicity was assessed by a quantitative technique at three observation periods. Endion was highly cytotoxic, causing a significant decrease in cell density. Ketac-Endo proved to be a very biocompatible material.
Beltes
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P
Department of Dental Pathology and Therapeutics, Faculty of Dentistry, Aristotelion University of Thessaloniki, Greece.
Koulaouzidou
E
E
Kolokuris
I
I
Kortsaris
A H
AH
D000818:Animals; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D006224:Cricetinae; D005347:Fibroblasts / Q000187:drug effects; D005899:Glass Ionomer Cements / Q000633:toxicity*; D007668:Kidney / Q000166:cytology / Q000187:drug effects; D012387:Root Canal Filling Materials / Q000633:toxicity*; D013997:Time Factors
C098488:Endion; D005899:Glass Ionomer Cements; C074934:Ketac Endo; D012387:Root Canal Filling Materials