A novel restriction endonuclease UnbI, a neoschizomer of Sau96I from an unidentified psychrofilic bacterium from Antarctica is inhibited by phosphate ions.
A novel type II restriction endonuclease UnbI was isolated from an unidentified psychrofilic bacterial strain from Antarctica. UnbI recognizes and cleaves the sequence 5'-GGNCC-3', producing 5 nucleotide long sticky ends. In this respect it differs from its neoschizomer Sau96I and all other restriction enzymes recognizing this sequence. UnbI has a relatively low temperature optimum of 15 degrees C to 20 degrees C and its activity is completely inhibited by inorganic phosphate.
Kawalec
M
M
Department of Genetics, University of Warsaw, Poland.
Borsuk
P
P
Piechula
S
S
Stepień
P P
PP
D000864:Antarctic Regions; D001483:Base Sequence; D001665:Binding Sites; D003080:Cold Temperature; D004247:DNA / Q000235:genetics / Q000378:metabolism; D015252:Deoxyribonucleases, Type II Site-Specific / Q000037:antagonists & inhibitors* / Q000302:isolation & purification* / Q000378:metabolism; D016946:Gram-Negative Aerobic Rods and Cocci / Q000201:enzymology* / Q000302:isolation & purification; D008969:Molecular Sequence Data; D010710:Phosphates / Q000494:pharmacology; D013379:Substrate Specificity
D010710:Phosphates; D004247:DNA; C113137:endodeoxyribonuclease UnbI; D015252:Deoxyribonucleases, Type II Site-Specific