[The inhibitory effect of TNF-alpha on alkaline phosphatase activity of human periodontal ligament fibroblasts].
The present investigation was to identify the impact of recombinant human tumor necrosis factor-alpha (TNF-ALPHA) on alkaline phosphatase (ALP) activity of cultured human periodontal ligament fibroblasts (HPLF). It was found that, after treatment with TNF-alpha, HPLF in the experimental groups showed significantly lower ALP activity than in the blank control groups (P < 0.01). Further, the ALP activity was observed to decrease progressively with increasing TNF-alpha concentrations. These findings indicate that TNF-alpha possesses a remarkable concentration-dependent inhibitory effect on human periodontal ligament fibroblasts.
Niu
Z
Z
School of Stomatology, Fourth Military Medical University, Xian.
Shi
J
J
Xiao
M
M
D000293:Adolescent; D000469:Alkaline Phosphatase / Q000378:metabolism*; D002478:Cells, Cultured; D002648:Child; D005347:Fibroblasts / Q000201:enzymology; D006801:Humans; D010513:Periodontal Ligament / Q000166:cytology / Q000201:enzymology*; D011994:Recombinant Proteins / Q000494:pharmacology; D014409:Tumor Necrosis Factor-alpha / Q000494:pharmacology*
D011994:Recombinant Proteins; D014409:Tumor Necrosis Factor-alpha; D000469:Alkaline Phosphatase