title: X-ray microscopic visualization of specific labeling of adhesive molecule CD36 and cytoadherence by Plasmodium falciparum infected erythrocytes.
abstract: We investigated the cytoadherence of Plasmodium falciparum infected erythrocytes to target cells that express CD36 by soft x-ray microscopy. Using immunogold beads enhanced with silver, we localized CD36 on the surface of intact melanoma cells and throughout Triton extracted melanoma cells. We examined the orientation of parasites within erythrocytes that bound to target cells, and the interactions between the red cell membrane and the target cell, and we confirmed that fibrillar structures on the surface of melanoma and endothelial cells can be involved in the association between infected erythrocytes and melanoma cells or endothelial cells.
authors.affiliation: Life Sciences Division, Lawrence Berkeley National Laboratory, Berkeley, CA.
authors.forename: J
authors.initials: J
authors.lastname: Yeung
authors.forename: J T
authors.initials: JT
authors.lastname: Brown
authors.forename: A
authors.initials: A
authors.lastname: Nair
authors.forename: E
authors.initials: E
authors.lastname: Meites
authors.forename: R L
authors.initials: RL
authors.lastname: Coppel
authors.forename: N
authors.initials: N
authors.lastname: Mohandas
authors.forename: W
authors.initials: W
authors.lastname: Meyer-Ilse
authors.forename: C
authors.initials: C
authors.lastname: Magowan
mesh_terms: D000818:Animals; D018955:CD36 Antigens / Q000032:analysis* / Q000276:immunology; D002448:Cell Adhesion; D004577:Electron Probe Microanalysis; D004730:Endothelium, Vascular / Q000276:immunology / Q000469:parasitology; D004912:Erythrocytes / Q000469:parasitology* / Q000502:physiology / Q000648:ultrastructure; D019084:Fluorescent Antibody Technique, Indirect; D006801:Humans; D008545:Melanoma / Q000276:immunology / Q000469:parasitology / Q000648:ultrastructure; D010963:Plasmodium falciparum / Q000502:physiology* / Q000648:ultrastructure; D014407:Tumor Cells, Cultured
chemical_list: D018955:CD36 Antigens