Freeze-dried human leukocytes stabilized with uranyl acetate during low temperature embedding or with OsO4 vapor after embedding.
Two new simple stabilization procedures for freeze-dried biological material are introduced which are compatible with low temperature embedding (LTE) in Lowicryl. The first method uses a Lowicryl K11M/HM20 mixture supplemented with 0.3% uranyl acetate for LTE. For the second method polymerized Lowicryl blocks containing the freeze-dried material are exposed to OsO4 vapor which penetrates into the Lowicryl block and stabilizes the embedded specimen. The quality of structural preservation is demonstrated with human leukocytes.
Edelmann
L
L
Universität des Saarlandes, Homburg-Saar, Germany.
Ruf
A
A
D000180:Acrylic Resins; D004396:Coloring Agents; D005612:Freeze Drying; D016591:Histocytological Preparation Techniques; D006801:Humans; D007963:Leukocytes, Mononuclear / Q000648:ultrastructure*; D008854:Microscopy, Electron; D009504:Neutrophils / Q000648:ultrastructure*; D009942:Organometallic Compounds; D009993:Osmium Tetroxide; D013696:Temperature; D016610:Tissue Embedding
D000180:Acrylic Resins; D004396:Coloring Agents; D009942:Organometallic Compounds; C005460:uranyl acetate; C070811:Lowicryl HM-20; D009993:Osmium Tetroxide