title: Improved methods for generation, sampling, and recovery of biological aerosols in filter challenge tests.
abstract: In preparation for filter efficiency tests and sampler comparison studies, methods of biological aerosol generation, sampling, and filter recovery were modified from previous studies. Methods described include (1) techniques for generating aerosols that reduced nuisance particles to negligible levels and increased the cell culturability of Mycobacterium abscessus by 30%, (2) sampling techniques that lowered the detectable range of biological particle size from 0.65 to 0.45 micron and reduced the sampling flow from the chamber from 28.3 to 1.5 L/min, and (3) development of methods to remove culturable organisms from respirator filter media. These methods were developed for filter challenge tests with M. abscessus and were applied to two other bacteria. They may also have application to a wider variety of organisms and bioaerosol assessments.
authors.affiliation: University of Minnesota, School of Public Health, Division of Environmental and Occupational Health, Minneapolis 55455, USA.
authors.forename: N V
authors.initials: NV
authors.lastname: McCullough
authors.forename: L M
authors.initials: LM
authors.lastname: Brosseau
authors.forename: D
authors.initials: D
authors.lastname: Vesley
authors.forename: J H
authors.initials: JH
authors.lastname: Vincent
mesh_terms: D000336:Aerosols / Q000032:analysis*; D002478:Cells, Cultured; D004784:Environmental Monitoring / Q000379:methods*; D004866:Equipment Contamination / Q000517:prevention & control*; D005374:Filtration / Q000295:instrumentation*; D006801:Humans; D008422:Materials Testing; D009161:Mycobacterium / Q000254:growth & development; D009330:Nebulizers and Vaporizers; D010316:Particle Size; D015203:Reproducibility of Results; D012134:Respiratory Protective Devices / Q000382:microbiology / Q000592:standards*
chemical_list: D000336:Aerosols