Fluoride-induced enhancement of diffusion in streptococcal model plaque biofilms.
It has been demonstrated that fluoride decreases the calcium-binding affinity of Streptococcus mutans and approximately doubles the calcium-binding capacity. To investigate the effect of this mechanism on calcium mobility in plaque, 45Ca flux was measured from a condensed films of S. mutans into tracer-free solution. Bacteria were suspended in pH 7.0 or 5.0 buffer including 0, 5, 10, 15 or 20 mmol/l Ca2+ carrier, with or without 5 mmol/l F- and with 45Ca and 3H-inulin. The appearance of 45Ca and 3H-inulin in carrier-containing but initially tracer-free buffer was measured and extracellular fraction (Ve) and bound calcium were calculated. As the ratio (R) of bound to free Ca2+ approached zero at high [Ca2+], the measured diffusion coefficient (rDe) approached the effective diffusion coefficient (De), such that: rDe = De/(1+R). Fluoride increased the rate of calcium diffusion by a reduction in the binding affinity. This work demonstrates that fluoride significantly increases mobility in plaque; this may increase the rate at which calcium is transported between plaque and an underlying lesion and so promote remineralization. This mechanism could also increase the penetration of bacteriocides and suggests a novel method for biofilm treatment.
Rose
R K
RK
MRC Dental Group, The Dental School, University of Bristol, UK. Keith.Rose@bristol.ac.uk
Turner
S J
SJ
D000704:Analysis of Variance; D000900:Anti-Bacterial Agents / Q000493:pharmacokinetics; D018441:Biofilms / Q000187:drug effects*; D001692:Biological Transport / Q000187:drug effects; D002021:Buffers; D002118:Calcium / Q000378:metabolism; D002132:Calcium Radioisotopes; D002327:Cariostatic Agents / Q000494:pharmacology*; D003773:Dental Plaque / Q000382:microbiology*; D004058:Diffusion; D005459:Fluorides / Q000494:pharmacology*; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007444:Inulin / Q000378:metabolism; D019275:Radiopharmaceuticals; D013295:Streptococcus mutans / Q000187:drug effects* / Q000378:metabolism; D014089:Tooth Remineralization; D014316:Tritium
D000900:Anti-Bacterial Agents; D002021:Buffers; D002132:Calcium Radioisotopes; D002327:Cariostatic Agents; D019275:Radiopharmaceuticals; D014316:Tritium; D007444:Inulin; D005459:Fluorides; D002118:Calcium