Detection of ABH blood group antigens from minute human hairs using polyvinylidene difluoride membrane with elution--ELISA method.
A sensitive and simple method for ABH blood grouping of human hairs using a polyvinylidene difluoride (PVDF) membrane was described. The hairs sensitized with monoclonal antibodies were applied onto the membrane and heated at 60 degrees C on a hot plate. When the membrane was examined with ELISA using a peroxidase-conjugated anti-IgM antibody, group-specific streaky stains were perceived on the membrane, because the eluates directly bound to the membrane. The present method could be available to the detection of minute antigens of short hairs.
Biwasaka
H
H
Department of Legal Medicine, Iwate Medical University School of Medicine, Morioka, Japan.
Nakayashiki
N
N
Aoki
Y
Y
D000017:ABO Blood-Group System / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D005260:Female; D006197:Hair / Q000276:immunology*; D006801:Humans; D007519:Isoantigens / Q000032:analysis*; D008297:Male; D008567:Membranes, Artificial; D011145:Polyvinyls; D005465:Fluorocarbon Polymers
D000017:ABO Blood-Group System; D007519:Isoantigens; D008567:Membranes, Artificial; D011145:Polyvinyls; C024865:polyvinylidene fluoride; D005465:Fluorocarbon Polymers