Thrombospondin-1 and its CSVTCG-specific receptor in wound healing and cancer.
Growth factors play a crucial role in the regulation of cellular proliferation and matrix degradation in wound healing and cancer. We have shown that thrombospondin 1 (TSP-1) and its cysteine-serine-valine-threonine-cysteine-glycine (CSVTCG)-specific receptor play a key role in cell invasion and matrix degradation in different carcinomas. The present study was done to determine whether TSP-1 and its receptor show a similar pattern of expression in wound healing and cancer. Expression and localization of TSP-1 and its receptor were determined in fetal wounds, adult burn wounds, and different human malignancies by immunohistochemical staining and computerized image analysis. In healing wounds, TSP-1 was expressed in the stroma early in the process, followed by a steep decline. The TSP-1 receptor localized to neovessels and highly proliferating cells (i.e., fibroblasts, basal cells), its levels remaining relatively constant. Cancer cells and tumor-associated microvessels expressed the TSP-1 receptor, whereas TSP-1 localized predominantly to the tumor-associated stroma. These data suggest a critical role for TSP-1 and its CSVTCG-specific receptor in wound healing and cancer.
Roth
J J
JJ
Department of Pathology and Surgery, Allegheny University of the Health Sciences, Philadelphia, PA 19102, USA.
Albo
D
D
Rothman
V L
VL
Longaker
M T
MT
Granick
M S
MS
Long
C D
CD
Solomon
M P
MP
Tuszynski
G P
GP
D000328:Adult; D000818:Animals; D001943:Breast Neoplasms / Q000378:metabolism* / Q000473:pathology; D004848:Epithelium / Q000473:pathology; D005260:Female; D006801:Humans; D007091:Image Processing, Computer-Assisted; D007150:Immunohistochemistry; D011956:Receptors, Cell Surface / Q000096:biosynthesis / Q000378:metabolism*; D012756:Sheep; D019700:Thrombospondin 1 / Q000096:biosynthesis / Q000378:metabolism*; D013997:Time Factors; D014945:Wound Healing / Q000502:physiology*
D011956:Receptors, Cell Surface; D019700:Thrombospondin 1