[Application of monoclonal antibody immuno-colloide gold probe in the study of porphyromonas gingivalis].
Immuno-electron localization of McAb BGG9 was specific for Porphyromonas gingivalis labeled with colloide gold. The monoclonal antibody immuno-colloide gold probe reacted with Porphyromonas gingivalis and 5 species of black pigmented bacteroides groups. The bacteria were fixed and embbed in Epon. sections were examined under transmission electron microscope, and the gold granular was observed near Porphyromonas gingivalis cell membrane. Our conclusion suggests that monoclonal antibody immuno-colloide gold probe is useful tool for studying Porphyromonas gingivalis.
Xu
L
L
Department of Stomatology, Hebei Medical College, Shijiazhuang.
Xue
Y
Y
Shi
J
J
D000907:Antibodies, Bacterial / Q000032:analysis; D000911:Antibodies, Monoclonal / Q000032:analysis*; D017979:Gold Colloid; D016253:Microscopy, Immunoelectron; D016966:Porphyromonas gingivalis / Q000302:isolation & purification* / Q000648:ultrastructure
D000907:Antibodies, Bacterial; D000911:Antibodies, Monoclonal; D017979:Gold Colloid