Analysis of the T cell receptor variability of tumor-infiltrating lymphocytes in colorectal carcinomas.
While there is good clinical and experimental evidence for immunological tumor control in some tumors--malignant melanomas, for instance--the immunogenicity of colorectal carcinoma (CRC) still remains unsettled. We examined surgical specimens from 4 CRC patients for T cell clones among tumor-infiltrating lymphocytes (TIL). The growth of specific lymphocyte clones in a tumor indicates an immunological response in vivo. We used a T cell receptor V beta family-specific semiquantitative PCR with additional sequencing to examine TIL for clonal expansion. In CRC, specific T cell clones could not be demonstrated. However, we observed a predominance of V beta 9 in 3 of 4 tumors.
Baier
P K
PK
Abteilung für Allgemeinchirurgie mit Poliklinik, Chirurgische Universitätsklinik, Freiburg, Deutschland.
Wimmenauer
S
S
Hirsch
T
T
von Specht
B U
BU
von Kleist
S
S
Keller
H
H
Farthmann
E H
EH
D000230:Adenocarcinoma / Q000276:immunology*; D002999:Clone Cells / Q000737:chemistry / Q000166:cytology / Q000276:immunology; D015179:Colorectal Neoplasms / Q000276:immunology*; D005260:Female; D006801:Humans; D016246:Lymphocytes, Tumor-Infiltrating / Q000737:chemistry / Q000166:cytology* / Q000276:immunology; D008297:Male; D008875:Middle Aged; D011948:Receptors, Antigen, T-Cell / Q000276:immunology*; D017421:Sequence Analysis
D011948:Receptors, Antigen, T-Cell