Reduction of corneal edema in endotoxin-induced uveitis after application of L-NAME as nitric oxide synthase inhibitor in rats by iontophoresis.
OBJECTIVE
To investigate the involvement of the cornea during endotoxin-induced uveitis (EIU) in the rat and the effect of Ngamma-nitro-L-arginine methyl ester (L-NAME) as nitric oxide synthase (NOS) inhibitor, administered by iontophoresis.


METHODS
EIU was induced in Lewis rats that were killed at 8 and 16 hours after lipopolysaccharide (LPS) injection. The severity of uveitis was evaluated clinically at 16 hours, and nitrite levels were evaluated in the aqueous humor at 8 hours. Corneal thickness was measured, 16 hours after LPS injection, on histologic sections using an image analyzer. Transmission electron microscopy (TEM) was used for fine analysis of the cornea. Transcorneoscleral iontophoresis of L-NAME (100 mM) was performed either at LPS injection or at 1 and 2 hours after LPS injection.


RESULTS
At 16 hours after LPS injection, mean corneal thickness was 153.7+/-5.58 microm in the group of rats injected with LPS (n=8) compared with 126.89+/-11.11 microm in the saline-injected rats (n=8) (P < 0.01). TEM showed stromal edema and signs of damage in the endothelial and epithelial layers. In the group of rats treated by three successive iontophoreses of L-NAME (n=8), corneal thickness was 125.24+/-10.36 microm compared with 146.76+/-7.52 microm in the group of rats treated with iontophoresis of saline (n=8), (P=0.015). TEM observation showed a reduction of stromal edema and a normal endothelium. Nitrite levels in the aqueous humor were significantly reduced at 8 hours by L-NAME treatment (P=0.03). No effect on corneal edema was observed after a single iontophoresis of L-NAME at LPS injection (P=0.19). Iontophoresis of saline by itself induced no change in corneal thickness nor in TEM structure analysis compared with normal rats.


CONCLUSIONS
Corneal edema is observed during EIU. This edema is significantly reduced by three successive iontophoreses of L-NAME, which partially inhibited the inflammation. A role of nitric oxide in the corneal endothelium functions may explain the antiedematous effect of L-NAME.
Behar-Cohen
F F
FF
Hôpital Hôtel-Dieu de Paris, France.
Savoldelli
M
M
Parel
J M
JM
Goureau
O
O
Thillaye-Goldenberg
B
B
Courtois
Y
Y
Pouliquen
Y
Y
de Kozak
Y
Y
D000818:Animals; D001082:Aqueous Humor / Q000378:metabolism; D003315:Cornea / Q000187:drug effects / Q000648:ultrastructure; D015715:Corneal Edema / Q000139:chemically induced / Q000473:pathology / Q000517:prevention & control*; D004791:Enzyme Inhibitors / Q000008:administration & dosage*; D007478:Iontophoresis; D008070:Lipopolysaccharides; D019331:NG-Nitroarginine Methyl Ester / Q000008:administration & dosage*; D019001:Nitric Oxide Synthase / Q000037:antagonists & inhibitors; D009573:Nitrites / Q000378:metabolism; D051381:Rats; D011917:Rats, Inbred Lew; D012486:Salmonella typhimurium; D014606:Uveitis, Anterior / Q000139:chemically induced* / Q000473:pathology
D004791:Enzyme Inhibitors; D008070:Lipopolysaccharides; D009573:Nitrites; D019001:Nitric Oxide Synthase; D019331:NG-Nitroarginine Methyl Ester