title: The acidophilic nature of neuronal Golgi impregnation.
abstract: The mechanisms of Golgi impregnation of neurons has remained enigmatic for decades. Recently, it was suggested that divalent (di)chromate anions play a role in the Golgi impregnation process. Therefore, we incubated slices of (para)formaldehyde-fixed rat brain tissue in solutions of potassium (di)chromate, phosphate, chloride or nitrate at pH 6 or 7. Slices were then immersed in solutions of silver nitrate and processed for light microscopical analysis. At pH 6, dichromate probes resulted in dense and homogeneous impregnation of neuronal cytoplasm (typical impregnation). At pH 7, chromate probes showed solely partial cytoplasmic and heavy nuclear-region neuron impregnation (atypical impregnation). Phosphate probes at pH 6 resulted in typical impregnation, whereas at pH 7 phosphate probes gave atypical impregnation. Both at pH 6 and 7, chloride and nitrate probes did not yield any Golgi impregnation. These findings confirmed the pH-dependence of silver-chromate Golgi impregnation as well as the correctness of corresponding acidic silver-phosphate impregnation. Our study revealed a previously unknown, strong anion-dependence of Golgi impregnation, suggesting that hydrogenated monovalent anions are carriers of the neuron impregnation.
authors.affiliation: Institute of Histology, Faculty of Medicine, University of Belgrade, Yugoslavia.
authors.forename: B D
authors.initials: BD
authors.lastname: Stefanović
authors.forename: D
authors.initials: D
authors.lastname: Ristanović
authors.forename: D
authors.initials: D
authors.lastname: Trpinac
authors.forename: V
authors.initials: V
authors.lastname: Dordević-Camba
authors.forename: V
authors.initials: V
authors.lastname: Lacković
authors.forename: V
authors.initials: V
authors.lastname: Bumbasirević
authors.forename: M
authors.initials: M
authors.lastname: Obradović
authors.forename: R
authors.initials: R
authors.lastname: Basić
authors.forename: M
authors.initials: M
authors.lastname: Cetković
mesh_terms: D000818:Animals; D001921:Brain / Q000166:cytology; D002840:Chromates; D005557:Formaldehyde; D006863:Hydrogen-Ion Concentration; D008297:Male; D015335:Molecular Probes; D009474:Neurons / Q000166:cytology / Q000378:metabolism*; D009566:Nitrates; D010710:Phosphates; D011108:Polymers; D011189:Potassium Chloride; D017680:Potassium Compounds; D051381:Rats; D017208:Rats, Wistar; D012835:Silver Nitrate; D013194:Staining and Labeling / Q000379:methods*; D016707:Tissue Fixation
chemical_list: D002840:Chromates; D015335:Molecular Probes; D009566:Nitrates; D010710:Phosphates; D011108:Polymers; D017680:Potassium Compounds; D005557:Formaldehyde; C027373:potassium chromate(VI); D011189:Potassium Chloride; D012835:Silver Nitrate; C013216:potassium phosphate; C023844:potassium nitrate; C003043:paraform