title: Ectopic expression of lunatic Fringe leads to downregulation of Serrate-1 in the developing chick neural tube; analysis using in ovo electroporation transfection technique.
abstract: Lunatic Fringe (l-Fng) is one of the vertebrate homologues of Drosophila Fringe, which interacts with the Notch signal pathway and regulates activation of the Notch ligands, Delta and Serrate. To elucidate the roles of l-Fng in vertebrate neurogenesis, we transfected chick l-Fng (C-l-Fng) to chick neural tube using the in ovo electroporation technique and examined the subsequent changes in expression of Notch-related genes. We observed downregulation of C-Serrate-1 by ectopic C-l-Fng expression which implied that C-l-Fng acts on the vertebrate Notch pathway to regulate the expression of its ligand.
authors.affiliation: Department of Oral Pathology, Faculty of Dentistry, Tokyo Medical and Dental University, Japan.
authors.forename: K
authors.initials: K
authors.lastname: Sakamoto
authors.forename: H
authors.initials: H
authors.lastname: Nakamura
authors.forename: M
authors.initials: M
authors.lastname: Takagi
authors.forename: S
authors.initials: S
authors.lastname: Takeda
authors.forename: K
authors.initials: K
authors.lastname: Katsube
mesh_terms: D000818:Animals; D030161:Avian Proteins; D002135:Calcium-Binding Proteins; D002642:Chick Embryo; D015536:Down-Regulation / Q000235:genetics*; D029721:Drosophila Proteins; D004331:Drosophila melanogaster; D004566:Electrodes; D018274:Electroporation / Q000379:methods; D018507:Gene Expression Regulation, Developmental; D016695:Glycosyltransferases; D036341:Intercellular Signaling Peptides and Proteins; D000072100:Jagged-1 Protein; D008565:Membrane Proteins / Q000096:biosynthesis* / Q000235:genetics; D009420:Nervous System / Q000196:embryology* / Q000378:metabolism*; D014176:Protein Biosynthesis; D011506:Proteins / Q000235:genetics* / Q000502:physiology; D000072099:Serrate-Jagged Proteins; D015398:Signal Transduction / Q000235:genetics; D014162:Transfection / Q000379:methods
chemical_list: D030161:Avian Proteins; D002135:Calcium-Binding Proteins; D029721:Drosophila Proteins; D036341:Intercellular Signaling Peptides and Proteins; D000072100:Jagged-1 Protein; D008565:Membrane Proteins; D011506:Proteins; C000606278:Ser protein, Drosophila; D000072099:Serrate-Jagged Proteins; D016695:Glycosyltransferases; C498394:LFNG protein, Gallus gallus