Does airway responsiveness increase after skin sensitisation to 3-carene: a study in isolated guinea pig lungs.
Guinea pigs were sensitised by dermal exposure to 3-carene according to the modified cumulative contact enhancement test (CCET) protocol. Lungs from sensitised and non-sensitised animals were then perfused with buffer and exposed for a period of 10 min to two different air concentrations of 3-carene, 600 and 3000 mg/m3. 3-Carene caused a statistically significant bronchoconstriction even at the relatively low concentration of 600 mg/m3 and the constriction was dose dependent. 600 mg/m3 of 3-carene caused a reduction of 19% in conductance capacity and 16% in compliance capacity. 3000 mg/m3 of 3-carene decreased lung compliance and conductance by 43 and 31%, respectively. The lungs from sensitised animals tended to show a greater response than lungs obtained from control animals. The lower concentration of 3-carene is close to and may even be below, occupational limit values in Sweden, Germany and USA.
Låstbom
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Division of Inhalation Toxicology, Institute of Environmental Medicine, Karolinska Institutet, Stockholm, Sweden.
Boman
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Camner
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Ryrfeldt
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D000485:Allergens / Q000008:administration & dosage* / Q000494:pharmacology; D000818:Animals; D000081008:Bicyclic Monoterpenes; D004342:Drug Hypersensitivity / Q000276:immunology*; D005260:Female; D006168:Guinea Pigs; D066298:In Vitro Techniques; D008168:Lung / Q000187:drug effects / Q000276:immunology*; D008297:Male; D039821:Monoterpenes; D010477:Perfusion; D012129:Respiratory Function Tests; D012867:Skin / Q000276:immunology*; D013729:Terpenes / Q000008:administration & dosage* / Q000494:pharmacology
D000485:Allergens; D000081008:Bicyclic Monoterpenes; D039821:Monoterpenes; D013729:Terpenes; C030218:3-carene