Apoptosis and expression of DNA repair proteins in ischaemic brain injury in man.
We examined the timing of apoptosis and the expression of the DNA repair proteins poly(ADP-ribose) polymerase (PARP) and Ku80 in sections of frontal and temporal lobes from patients who had suffered severe brain ischaemia due to a cardiac arrent. In situ end-labelling (ISEL) was used to detect apoptotic cells, and immunohistochemistry to assess PARP and Ku80. ISEL of scattered neurons and glia was demonstrable predominantly during the first 24 h after ischaemia. PARP and Ku80 immunoreactivity increased markedly after cerebral ischaemia, PARP particularly in the regions of greatest susceptibility to hypoxic injury: the CA1 field of the hippocampus and the depths of neocortical sulci. The up-regulation of PARP is in keeping with experimental observations concerning the key role of this enzyme in mediating ischaemic cell death.
Love
S
S
Department of Neuropathology, Frenchay Hospital, Bristol, UK.
Barber
R
R
Wilcock
G K
GK
D000328:Adult; D000368:Aged; D034961:Antigens, Nuclear; D017209:Apoptosis / Q000502:physiology*; D002545:Brain Ischemia / Q000378:metabolism*; D002648:Child; D004265:DNA Helicases; D004260:DNA Repair; D004268:DNA-Binding Proteins / Q000096:biosynthesis*; D005260:Female; D005625:Frontal Lobe / Q000098:blood supply / Q000378:metabolism; D006801:Humans; D007150:Immunohistochemistry; D017403:In Situ Hybridization; D000072200:Ku Autoantigen; D008297:Male; D008875:Middle Aged; D009419:Nerve Tissue Proteins / Q000096:biosynthesis*; D009687:Nuclear Proteins / Q000096:biosynthesis*; D011065:Poly(ADP-ribose) Polymerases / Q000096:biosynthesis*; D013702:Temporal Lobe / Q000098:blood supply / Q000378:metabolism
D034961:Antigens, Nuclear; D004268:DNA-Binding Proteins; D009419:Nerve Tissue Proteins; D009687:Nuclear Proteins; D011065:Poly(ADP-ribose) Polymerases; D004265:DNA Helicases; C106893:XRCC5 protein, human; C000606509:Xrcc6 protein, human; D000072200:Ku Autoantigen