Three mitochondrial DNA restriction polymorphisms in elite endurance athletes and sedentary controls.
This study examined the associations between elite endurance athlete (EEA) status and three mitochondrial DNA (mtDNA) restriction fragment length polymorphisms (RFLPs) in the subunit 5 of the NADH dehydrogenase (MTND5) locus and one in the D-loop region. A group of 125 Caucasian male EEA well endowed with the phenotypic expression of VO2max (78.9 +/- 3.8 mL x kg(-1) x min(-1), mean +/- SD) and 65 sedentary controls (SCON: VO2max = 39.8 +/- 8.2 mL x kg(-1) x min(-1)) participated in the study. VO2max was determined during an incremental exercise test on a cycle ergometer or a motor-driven treadmill. mtDNA was extracted from white blood cells or lymphoblastoid cell lines and specific regions were amplified by the polymerase chain reaction. The Pearson Chi-square statistic test and Fisher exact test revealed no significant association (P > 0.05) between any of the three mtDNA RFLPs and EEA status. The MTND5-BamHI RFLP at bp 13,470 (morph 3) was found in 12.8% of the EEA and 12.3% of the SCON (chi2 = 0.009, P = 0.92). The prevalence of the MTND5-Ncil RFLP at bp 13,364 (morph 2) was 12.9% and 14% for the EEA and SCON, respectively (chi2 = 0.043, P = 0.83). The D-loop-KpnI RFLP at bp 16,133 (morph 1) was found in 5.8% of the EEA and in 1.6% of the SCON (Fisher exact test = 1.80, P = 0.18). The MTND5-HincII RFLP at bp 12,406 (morph 1) was not present in this study sample. These results indicate no evidence for a difference in the frequency of two polymorphic restriction sites in the subunit 5 of the NADH dehydrogenase gene of mtDNA and one in the D-loop region between elite endurance athletes and sedentary controls.
Rivera
M A
MA
Physical Activity Sciences Laboratory, Laval University, Québec, Canada.
Wolfarth
B
B
Dionne
F T
FT
Chagnon
M
M
Simoneau
J A
JA
Boulay
M R
MR
Song
T M
TM
Perusse
L
L
Gagnon
J
J
Leon
A S
AS
Rao
D C
DC
Skinner
J S
JS
Wilmore
J H
JH
Keul
J
J
Bouchard
C
C
D000293:Adolescent; D000328:Adult; D000595:Amino Acid Sequence; D004272:DNA, Mitochondrial; D014644:Genetic Variation; D006801:Humans; D008297:Male; D008875:Middle Aged; D008969:Molecular Sequence Data; D009245:NADH Dehydrogenase / Q000235:genetics*; D010101:Oxygen Consumption; D010807:Physical Endurance / Q000235:genetics*; D010809:Physical Fitness; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length; D015183:Restriction Mapping
D004272:DNA, Mitochondrial; D009245:NADH Dehydrogenase