Virulence of entomopathogenic nematodes to the western masked chafer Cyclocephala hirta (Coleoptera: Scarabaeidae).
Predictability is a key challenge in biological control of white grubs with entomopathogenic nematodes. Most field test failures have been attributed to the use of inappropriate nematode strains. We evaluated several species and strains of entomopathogenic nematodes (Heterorhabditidae and Steinernematidae) against chafer Cyclocephala hirta in a soil and pot bioassay at 25 degrees C. The NJ65 strain of Steinernema glaseri, isolated from New Jersey, outperformed all other steinernematid and heterorhabditid nematodes, resulting in a 76.5% larval mortality within 3 d of treatment at 125 nematodes per larva. After 6 days of treatment, 4 strains of S. glaseri (NJ21, NJ29, NJ42, and NJ65) achieved 100% larval mortality. Other strains that caused > 80% larval mortality after 6 d of treatment included NJ32, NJ40, and NJ41 of S. glaseri, and Chino Hill, Merced, and Nebraska strains of Heterorhabditis sp. Steinernema anomali (Ryazan), Steinernema kushidai (Hamakita), Heterorhabditis megidis (HO1), and H. bacteriophora (HP88) caused only 45, 55, 60, and 66.7% larval mortality, respectively. Steinernema feltiae (Argentina strain) caused only 16% larval mortality, and Steinernema carpocapsae (All and Mexican strains) and Steinernema scapterisci (Colon strain) were nonpathogenic to C. hirta. Steinernema riobravis caused no larval mortality at 25 degrees C, but inflicted 45-71% mortality at 30 degrees C. Our studies indicate that S. glaseri and Heterorhabditis spp. are most virulent among entomopathogenic nematodes toward C. hirta larvae and certain strains of S. glaseri are superior to Heterorhabditis spp.
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Department of Entomology, OARDC, Ohio State University, Wooster 44691-4096, USA.
Grewal
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D000818:Animals; D001517:Coleoptera; D007814:Larva; D010572:Pest Control, Biological; D006109:Poaceae; D017172:Rhabditoidea; D013045:Species Specificity