cDNA cloning, gene expression and secretion of chitinase in winged bean.
cDNA for an acidic class III chitinase (ChitW1) was isolated from winged bean cells. The chitinase was abundantly secreted at later stages of cell culture, when levels of ChitW1 mRNA were also high. The gene was strongly expressed in roots, but a class I chitinase was strongly expressed in leaves.
Esaka
M
M
Faculty of Applied Biological Science, Hiroshima University, Japan.
Teramoto
T
T
D000595:Amino Acid Sequence; D002478:Cells, Cultured; D002688:Chitinases / Q000235:genetics* / Q000378:metabolism; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics; D007887:Fabaceae / Q000201:enzymology / Q000235:genetics*; D018506:Gene Expression Regulation, Plant; D008969:Molecular Sequence Data; D010940:Plant Proteins; D010946:Plants, Medicinal; D012333:RNA, Messenger / Q000032:analysis; D018749:RNA, Plant / Q000032:analysis; D017386:Sequence Homology, Amino Acid; D014018:Tissue Distribution
D018076:DNA, Complementary; D010940:Plant Proteins; D012333:RNA, Messenger; D018749:RNA, Plant; D002688:Chitinases; C110892:chitinase, class III