title: Genetic evaluation of suspected cases of transient HIV-1 infection of infants.
abstract: Detection of human immunodeficiency virus-type 1 (HIV-1) on only one or a few occasions in infants born to infected mothers has been interpreted to indicate that infection may be transient rather than persistent. Forty-two cases of suspected transient HIV-1 viremia among 1562 perinatally exposed seroreverting infants and one mother were reanalyzed. HIV-1 env sequences were not found in specimens from 20; in specimens from 6, somatic genetic analysis revealed that specimens were mistakenly attributed to an infant; and in specimens from 17, phylogenetic analysis failed to demonstrate the expected linkage between the infant's and the mother's virus. These findings argue that transient HIV-1 infection, if it exists, will only rarely be satisfactorily documented.
authors.affiliation: Department of Pediatrics, University of Rochester, Rochester, NY 14642, USA. lfrenkel@u.washington.edu
authors.forename: L M
authors.initials: LM
authors.lastname: Frenkel
authors.forename: J I
authors.initials: JI
authors.lastname: Mullins
authors.forename: G H
authors.initials: GH
authors.lastname: Learn
authors.forename: L
authors.initials: L
authors.lastname: Manns-Arcuino
authors.forename: B L
authors.initials: BL
authors.lastname: Herring
authors.forename: M L
authors.initials: ML
authors.lastname: Kalish
authors.forename: R W
authors.initials: RW
authors.lastname: Steketee
authors.forename: D M
authors.initials: DM
authors.lastname: Thea
authors.forename: J E
authors.initials: JE
authors.lastname: Nichols
authors.forename: S L
authors.initials: SL
authors.lastname: Liu
authors.forename: A
authors.initials: A
authors.lastname: Harmache
authors.forename: X
authors.initials: X
authors.lastname: He
authors.forename: D
authors.initials: D
authors.lastname: Muthui
authors.forename: A
authors.initials: A
authors.lastname: Madan
authors.forename: L
authors.initials: L
authors.lastname: Hood
authors.forename: A T
authors.initials: AT
authors.lastname: Haase
authors.forename: M
authors.initials: M
authors.lastname: Zupancic
authors.forename: K
authors.initials: K
authors.lastname: Staskus
authors.forename: S
authors.initials: S
authors.lastname: Wolinsky
authors.forename: P
authors.initials: P
authors.lastname: Krogstad
authors.forename: J
authors.initials: J
authors.lastname: Zhao
authors.forename: I
authors.initials: I
authors.lastname: Chen
authors.forename: R
authors.initials: R
authors.lastname: Koup
authors.forename: D
authors.initials: D
authors.lastname: Ho
authors.forename: B
authors.initials: B
authors.lastname: Korber
authors.forename: R J
authors.initials: RJ
authors.lastname: Apple
authors.forename: R W
authors.initials: RW
authors.lastname: Coombs
authors.forename: S
authors.initials: S
authors.lastname: Pahwa
authors.forename: N J
authors.initials: NJ
authors.lastname: Roberts
mesh_terms: D004279:DNA, Viral / Q000032:analysis / Q000235:genetics; D003951:Diagnostic Errors; D004866:Equipment Contamination; D005260:Female; D015752:Genes, env; D015658:HIV Infections / Q000276:immunology / Q000635:transmission / Q000821:virology*; D015497:HIV-1 / Q000235:genetics* / Q000302:isolation & purification*; D006801:Humans; D007223:Infant; D007231:Infant, Newborn; D018445:Infectious Disease Transmission, Vertical; D008969:Molecular Sequence Data; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D012367:RNA, Viral / Q000032:analysis; D013048:Specimen Handling; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology; D014766:Viremia / Q000821:virology
chemical_list: D004279:DNA, Viral; D012367:RNA, Viral