Metabolism of kalopanaxsaponin B and H by human intestinal bacteria and antidiabetic activity of their metabolites.
To investigate the relationship between the intestinal bacterial metabolism of kalopanaxsaponin B and H from Kalopanax pictus (Araliaceae), and their antidiabetic effect, kalopanaxsaponin B and H were metabolized by human intestinal microflora and the antidiabetic activity of their metabolites was measured. Human intestinal microflora metabolized kalopanaxsaponin B to kalopanaxsaponin A, hederagenin 3-O-alpha-L-arabinopyranoside and hederagenin. The main metabolites of kalopanaxsaponin B were kalopanaxsaponin A and hederagenin. Kalopanaxsaponin H was metabolized to kalopanaxsaponin A and I, hederagenin 3-O-alpha-L-arabinopyranoside and hederagenin. The main metabolites of kalopanaxsaponin H were kalopanaxsaponin I and hederagenin. Among kalopanaxsaponin B, H and their metabolites, kalopanaxsaponin A showed the most potent antidiabetic activity, followed by hederagenin. However, the main components, kalopanaxsaponin B and H, in K. pictus were inactive.
Kim
D H
DH
College of Pharmacy, Kyung-Hee University, Seoul, Korea.
Yu
K W
KW
Bae
E A
EA
Park
H J
HJ
Choi
J W
JW
D000818:Animals; D001786:Blood Glucose / Q000378:metabolism; D055598:Chemical Phenomena; D002627:Chemistry, Physical; D003921:Diabetes Mellitus, Experimental / Q000378:metabolism; D006801:Humans; D007004:Hypoglycemic Agents / Q000378:metabolism* / Q000494:pharmacology*; D007413:Intestinal Mucosa / Q000378:metabolism*; D007422:Intestines / Q000382:microbiology*; D008055:Lipids / Q000097:blood; D009682:Magnetic Resonance Spectroscopy; D008297:Male; D009828:Oleanolic Acid / Q000031:analogs & derivatives*; D051381:Rats; D017207:Rats, Sprague-Dawley; D012503:Saponins / Q000378:metabolism* / Q000494:pharmacology
D001786:Blood Glucose; D007004:Hypoglycemic Agents; D008055:Lipids; D012503:Saponins; C112799:kalopanax saponin H; C064714:kalopanax saponin B; D009828:Oleanolic Acid