title: Necator americanus (Nematoda: Ancylostomatidae) from Africa and Malaysia have different ITS-2 rDNA sequences.
abstract: The nucleotide sequences of the second internal transcribed spacer of rDNA were determined for adult worms of Necator americanus originating from Togo (Africa) and Sarawak (Malaysia). The length of the sequences of specimens from Togo (325 bp) were shorter than those from Sarawak (327 bp). There were six fixed genetic differences in the aligned sequences of N. americanus from Sarawak and Togo, excluding one or two polymorphic sites within the sequence of N. americanus from each geographical region. These findings suggest that there is either population variation in the sequence of N. americanus, or that N. americanus from the two countries may represent genetically distinct but morphologically similar (i.e. cryptic) species, however, comparison of the sequence differences among other hookworm species supports the latter conclusion.
authors.affiliation: Department of Veterinary Science, University of Melbourne, Werribee, Victoria, Australia.
authors.forename: A
authors.initials: A
authors.lastname: Romstad
authors.forename: R B
authors.initials: RB
authors.lastname: Gasser
authors.forename: P
authors.initials: P
authors.lastname: Nansen
authors.forename: A M
authors.initials: AM
authors.lastname: Polderman
authors.forename: N B
authors.initials: NB
authors.lastname: Chilton
mesh_terms: D000349:Africa; D000818:Animals; D001483:Base Sequence; D018509:DNA, Helminth / Q000235:genetics*; D004275:DNA, Ribosomal / Q000235:genetics*; D006801:Humans; D008296:Malaysia; D008969:Molecular Sequence Data; D017221:Necator americanus / Q000145:classification / Q000235:genetics* / Q000254:growth & development / Q000302:isolation & purification; D009332:Necatoriasis / Q000175:diagnosis / Q000469:parasitology*; D015183:Restriction Mapping; D016415:Sequence Alignment
chemical_list: D018509:DNA, Helminth; D004275:DNA, Ribosomal