Effect of Syzygium aromaticum extract on immediate hypersensitivity in rats.
We investigated the effect of aqueous extract of Syzygium aromaticum (L.) Merr. et Perry (Myrtaceae) flower bud (SAFB) on immediate hypersensitivity. SAFB inhibited compound 48/80-induced systemic anaphylaxis in rats (IC50 = 31.25 mg/kg, i.p.). SAFB also inhibited local immunoglobulin E (IgE)-mediated passive cutaneous anaphylactic reaction (IC50 = 17.78 mg/kg, i.v.; IC50 = 19.81 mg/kg, p.o.). When SAFB was pretreated at concentrations ranging from 25 to 1000 mg/kg, the serum histamine levels were reduced in a dose-dependent manner. Moreover, SAFB dose-dependently inhibited histamine release from rat peritoneal mast cells (RPMC) by compound 48/80 or anti-dinitrophenyl IgE. When SAFB was added, the level of cAMP in RPMC transiently and significantly increased about 47-fold at 10 s compared with that of basal cells. These results indicate that SAFB inhibits immediate hypersensitivity by inhibition of histamine release from mast cells in vivo and in vitro.
Kim
H M
HM
Department of Oriental Pharmacy, College of Pharmacy, Wonkwang University, Chonbuk, South Korea.
Lee
E H
EH
Hong
S H
SH
Song
H J
HJ
Shin
M K
MK
Kim
S H
SH
Shin
T Y
TY
D000818:Animals; D000242:Cyclic AMP / Q000378:metabolism; D004305:Dose-Response Relationship, Drug; D006632:Histamine / Q000097:blood; D006969:Hypersensitivity, Immediate / Q000139:chemically induced / Q000188:drug therapy*; D008407:Mast Cells / Q000187:drug effects / Q000378:metabolism; D010529:Peritoneal Cavity / Q000166:cytology; D010936:Plant Extracts / Q000627:therapeutic use*; D051381:Rats; D017208:Rats, Wistar; D003189:p-Methoxy-N-methylphenethylamine / Q000494:pharmacology
D010936:Plant Extracts; D003189:p-Methoxy-N-methylphenethylamine; D006632:Histamine; D000242:Cyclic AMP