The abilities of a Staphylococcus epidermidis wild-type strain and its slime-negative mutant to induce endocarditis in rabbits are comparable.
The abilities of a parent and mutant pair of Staphylococcus epidermidis strains, the slime-producing parent RP62A and its slime-negative mutant, to establish endocarditis in a rabbit model of aortic valve endocarditis and to accumulate and adhere to surfaces in vitro were compared. Vegetation titer and infection rate depended on the presence or absence of a catheter (P = 0.020) and on inoculum size (P < 0.001) but not on the infecting strain. The ability of the parent strain vis-à-vis its mutant to accumulate in vitro on surfaces as demonstrated in a slime test did not correlate with any enhancement in the development of endocarditis in the rabbit model. In vitro initial adherence rates were identical. Both isolates accumulated to the same reduced extent in vitro in the presence of serum, albumin, or gelatin. Adhesion was equally promoted by addition of fibronectin. These data suggest that the in vitro phenomenon of accumulation described as slime production in the absence of serum may not be an important virulence determinant in vivo.
Perdreau-Remington
F
F
Institute of Medical Microbiology and Hygiene, University of Cologne, Cologne, Germany. francoise@epi-center.ucsf.edu
Sande
M A
MA
Peters
G
G
Chambers
H F
HF
D000818:Animals; D001021:Aortic Valve; D001422:Bacterial Adhesion / Q000187:drug effects / Q000235:genetics; D001426:Bacterial Proteins / Q000235:genetics*; D002408:Catheters, Indwelling; D004697:Endocarditis, Bacterial / Q000209:etiology*; D005353:Fibronectins / Q000494:pharmacology; D006349:Heart Valve Diseases / Q000209:etiology*; D009154:Mutation; D011817:Rabbits; D013203:Staphylococcal Infections / Q000209:etiology*; D013212:Staphylococcus epidermidis / Q000235:genetics / Q000472:pathogenicity*
D001426:Bacterial Proteins; D005353:Fibronectins; C025431:extracellular slime proteins, bacterial