title: Characterization of two Sp1 binding sites of the human sex determining SRY promoter.
abstract: To investigate the molecular basis of the human SRY gene regulation, we have examined the significance of two potential binding sites for the transcription factor Sp1 (Sp1A: -124 to -131 and Sp1B: -147 to -154) by DNase I footprinting and gel mobility shift assays. Cotransfection experiments in Drosophila SL2 cells implicated Sp1 protein in the transcriptional activation of the SRY promoter.
authors.affiliation: Centre de Recherche de Biochimie Macromoléculaire, ERS155 CNRS, Montpellier, France.
authors.forename: M
authors.initials: M
authors.lastname: Desclozeaux
authors.forename: F
authors.initials: F
authors.lastname: Poulat
authors.forename: P
authors.initials: P
authors.lastname: de Santa Barbara
authors.forename: S
authors.initials: S
authors.lastname: Soullier
authors.forename: P
authors.initials: P
authors.lastname: Jay
authors.forename: P
authors.initials: P
authors.lastname: Berta
authors.forename: B
authors.initials: B
authors.lastname: Boizet-Bonhoure
mesh_terms: D000818:Animals; D001483:Base Sequence; D001665:Binding Sites / Q000235:genetics; D002460:Cell Line; D004247:DNA / Q000235:genetics / Q000378:metabolism; D018983:DNA Footprinting; D004268:DNA-Binding Proteins / Q000235:genetics*; D003850:Deoxyribonuclease I; D004330:Drosophila; D005260:Female; D006801:Humans; D008297:Male; D009687:Nuclear Proteins; D011401:Promoter Regions, Genetic; D019849:Sex Determination Processes; D051876:Sex-Determining Region Y Protein; D016329:Sp1 Transcription Factor / Q000378:metabolism*; D014157:Transcription Factors; D015533:Transcriptional Activation; D014162:Transfection
chemical_list: D004268:DNA-Binding Proteins; D009687:Nuclear Proteins; C497745:SRY protein, human; D051876:Sex-Determining Region Y Protein; D016329:Sp1 Transcription Factor; D014157:Transcription Factors; D004247:DNA; D003850:Deoxyribonuclease I