title: Identification and location on syndecan-1 core protein of the epitopes of B-B2 and B-B4 monoclonal antibodies.
abstract: Using a phage display peptide library, we characterized the epitope of two monoclonal antibodies reacting with syndecan-1: B-B2 and B-B4. The identified epitopes QDIT, for B-B2, and LPEV, for B-B4, were found to align with residues 36-39 and 90-93 of the mature protein, respectively. In contrast to B-B4, the B-B2 epitope is close to a potential glycosaminoglycan attachment site. Since syndecan-1 is heavily glycosylated and post-translational modifications are cell type specific, these results might explain the differences observed in the reactivity pattern of B-B2 and B-B4 and suggest that these monoclonal antibodies are useful probes to study cell surface exposed syndecan-1.
authors.affiliation: Diaclone, 1 bvd. A. Fleming, Besançon, France. jmdore@diaclone.com
authors.forename: J M
authors.initials: JM
authors.lastname: Dore
authors.forename: F
authors.initials: F
authors.lastname: Morard
authors.forename: N
authors.initials: N
authors.lastname: Vita
authors.forename: J
authors.initials: J
authors.lastname: Wijdenes
mesh_terms: D000595:Amino Acid Sequence; D000911:Antibodies, Monoclonal / Q000276:immunology*; D000918:Antibody Specificity; D018604:Epitope Mapping; D008562:Membrane Glycoproteins / Q000276:immunology*; D008969:Molecular Sequence Data; D019151:Peptide Library; D010455:Peptides / Q000276:immunology; D011509:Proteoglycans / Q000276:immunology*; D011994:Recombinant Proteins; D053667:Syndecans
chemical_list: D000911:Antibodies, Monoclonal; D008562:Membrane Glycoproteins; D019151:Peptide Library; D010455:Peptides; D011509:Proteoglycans; D011994:Recombinant Proteins; D053667:Syndecans