NADPH-diaphorase activity in nerves and Schwann cells in the periodontal ligament of rat incisor teeth.
The lingual portion of the incisor periodontal ligament demonstrated activity for nicotinamide adenosine dinucleotide phosphate (NADPH)-diaphorase. Schwann cells surrounding Ruffini-like endings coexpressed NADPH-diaphorase activity and immunoreactivity for inducible nitric oxide synthase. NADPH-diaphorase-positive nerve fibres which coexpressed immunoreactivity for neuronal nitric oxide synthase were in contact with Schwann cells surrounding Ruffini-like endings or terminated as free nerve endings. Neural NADPH-diaphorase activity could not be found in the tissues covering the labial portion of incisor tooth root. It is possible that nitric oxide in Schwann cells and nerves has functions specific to the incisor periodontal ligament.
Ichikawa
H
H
Second Department of Oral Anatomy, Okayama University Dental School, Japan.
Sugimoto
T
T
D000818:Animals; D016650:Fluorescein-5-isothiocyanate; D005455:Fluorescent Antibody Technique; D005456:Fluorescent Dyes; D007180:Incisor; D007527:Isoenzymes / Q000378:metabolism; D008297:Male; D008465:Mechanoreceptors / Q000201:enzymology / Q000648:ultrastructure; D016253:Microscopy, Immunoelectron; D009252:NADPH Dehydrogenase / Q000378:metabolism*; D009411:Nerve Endings / Q000201:enzymology / Q000648:ultrastructure; D009412:Nerve Fibers / Q000201:enzymology / Q000648:ultrastructure; D009474:Neurons / Q000201:enzymology / Q000648:ultrastructure; D009569:Nitric Oxide / Q000378:metabolism; D019001:Nitric Oxide Synthase / Q000378:metabolism; D010513:Periodontal Ligament / Q000294:innervation*; D051381:Rats; D017207:Rats, Sprague-Dawley; D009418:S100 Proteins / Q000378:metabolism; D012583:Schwann Cells / Q000201:enzymology* / Q000648:ultrastructure
D005456:Fluorescent Dyes; D007527:Isoenzymes; D009418:S100 Proteins; D009569:Nitric Oxide; D019001:Nitric Oxide Synthase; D009252:NADPH Dehydrogenase; D016650:Fluorescein-5-isothiocyanate