A high-copy-number ADE2-bearing plasmid for transformation of Candida glabrata.
The Candida glabrata ADE2 gene encoding aminoimidazole ribonucleotide (AIR) carboxylase (EC 4.1.1.21) was isolated by complementation of the ade2-1 mutation in Saccharomyces cerevisiae. The predicted amino acid (aa) sequence is 75% identical to that of S. cerevisiae. Integrative transformation was used to produce a C. glabrata strain bearing a deletion of ADE2 coding sequences. A high-copy-number shuttle vector bearing the ADE2 gene was constructed and contains a fragment of S. cerevisiae mitochondrial (mt) DNA that confers the ability to replicate autonomously in C. glabrata.
Hanic-Joyce
P J
PJ
Department of Chemistry, Biochemistry, Concordia University, 1455 de Maisonneuve Blvd. West, Montréal, Québec H3G 1M8, Canada. joycep@vax2.concordia.ca
Joyce
P B
PB
D002175:Candida / Q000235:genetics*; D002262:Carboxy-Lyases / Q000235:genetics*; D003001:Cloning, Molecular; D004274:DNA, Recombinant; D017353:Gene Deletion; D018628:Gene Dosage; D005800:Genes, Fungal / Q000235:genetics; D005822:Genetic Vectors / Q000235:genetics; D009154:Mutation / Q000235:genetics; D010957:Plasmids / Q000235:genetics*; D012441:Saccharomyces cerevisiae / Q000235:genetics; D014170:Transformation, Genetic
D004274:DNA, Recombinant; D002262:Carboxy-Lyases; C022378:phosphoribosylaminoimidazole carboxylase