title: Oligophrenin-1 encodes a rhoGAP protein involved in X-linked mental retardation.
abstract: Primary or nonspecific X-linked mental retardation (MRX) is a heterogeneous condition in which affected patients do not have any distinctive clinical or biochemical features in common apart from cognitive impairment. Although it is present in approximately 0.15-0.3% of males, most of the genetic defects associated with MRX, which may involve more than ten different genes, remain unknown. Here we report the characterization of a new gene on the long arm of the X-chromosome (position Xq12) and the identification in unrelated individuals of different mutations that are predicted to cause a loss of function. This gene is highly expressed in fetal brain and encodes a protein of relative molecular mass 91K, named oligophrenin-1, which contains a domain typical of a Rho-GTPase-activating protein (rhoGAP). By enhancing their GTPase activity, GAP proteins inactivate small Rho and Ras proteins, so inactivation of rhoGAP proteins might cause constitutive activation of their GTPase targets. Such activation is known to affect cell migration and outgrowth of axons and dendrites in vivo. Our results demonstrate an association between cognitive impairment and a defect in a signalling pathway that depends on a Ras-like GTPase.
authors.affiliation: INSERM U129-ICGM, Faculté de Médecine Cochin, Paris, France.
authors.forename: P
authors.initials: P
authors.lastname: Billuart
authors.forename: T
authors.initials: T
authors.lastname: Bienvenu
authors.forename: N
authors.initials: N
authors.lastname: Ronce
authors.forename: V
authors.initials: V
authors.lastname: des Portes
authors.forename: M C
authors.initials: MC
authors.lastname: Vinet
authors.forename: R
authors.initials: R
authors.lastname: Zemni
authors.forename: H
authors.initials: H
authors.lastname: Roest Crollius
authors.forename: A
authors.initials: A
authors.lastname: Carrié
authors.forename: F
authors.initials: F
authors.lastname: Fauchereau
authors.forename: M
authors.initials: M
authors.lastname: Cherry
authors.forename: S
authors.initials: S
authors.lastname: Briault
authors.forename: B
authors.initials: B
authors.lastname: Hamel
authors.forename: J P
authors.initials: JP
authors.lastname: Fryns
authors.forename: C
authors.initials: C
authors.lastname: Beldjord
authors.forename: A
authors.initials: A
authors.lastname: Kahn
authors.forename: C
authors.initials: C
authors.lastname: Moraine
authors.forename: J
authors.initials: J
authors.lastname: Chelly
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D002490:Central Nervous System / Q000196:embryology / Q000378:metabolism; D002874:Chromosome Mapping; D003598:Cytoskeletal Proteins; D005260:Female; D016368:Frameshift Mutation; D020558:GTP Phosphohydrolases / Q000378:metabolism; D019204:GTP-Binding Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D020690:GTPase-Activating Proteins; D018507:Gene Expression Regulation, Developmental; D008040:Genetic Linkage; D006160:Guanosine Triphosphate / Q000378:metabolism; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D008607:Intellectual Disability / Q000235:genetics*; D008297:Male; D008969:Molecular Sequence Data; D009687:Nuclear Proteins / Q000737:chemistry / Q000235:genetics*; D010375:Pedigree; D010750:Phosphoproteins / Q000737:chemistry / Q000235:genetics*; D016133:Polymerase Chain Reaction; D011506:Proteins / Q000378:metabolism; D011993:Recombinant Fusion Proteins / Q000378:metabolism; D017384:Sequence Deletion; D014178:Translocation, Genetic; D014960:X Chromosome / Q000235:genetics*; D020703:ras GTPase-Activating Proteins
chemical_list: D003598:Cytoskeletal Proteins; D020690:GTPase-Activating Proteins; D009687:Nuclear Proteins; C111952:OPHN1 protein, human; D010750:Phosphoproteins; D011506:Proteins; D011993:Recombinant Fusion Proteins; D020703:ras GTPase-Activating Proteins; C085379:rho GTPase-activating protein; D006160:Guanosine Triphosphate; D020558:GTP Phosphohydrolases; D019204:GTP-Binding Proteins