title: [Use of immunohistochemical analysis of antigen expression and exfoliative cytology of biliary epithelial cells in diagnosis of mechanical jaundice].
abstract: Imaging biliary strictures may suggest malignancy, but cytology can provide final diagnosis. In the present evaluation we studied the importance of immunohistochemical analysis of antigen expression in sensitivity and specificity of cytological evaluation of bile duct epithelium cells. Thirty three patients with biliary strictures were examined prospectively. The bile exfoliative cytology during endoscopic retrograde cholangio-pancreatography or surgery was obtained. Bile specimens were routinely stained with hematoxylin-eosin, examined by one experienced cytologist and were reported as positive or negative for malignant cells. In addition, the alterations in immunoperoxidase staining of carcinoembryonic antigen (mab T84,66), mucin (RA 96), cell membrane antigen (17-1A) and c-new were assessed. The results revealed a low sensitivity of both--traditional exfoliative cytology and the immunohistochemical analysis of antigen expression in diagnosis of biliary tract carcinoma (equally 25 and 31%, with the specificity of 92%). Appreciation of the samples with simultaneous involvement of the both studied methods make possible the diagnosis of the bile duct malignant lesions on 7 out of 17 cases (sensitivity 43%) with the specificity of 98%.
authors.affiliation: Klinika Chirurgii Ogólnej i Naczyń AM w Poznaniv.
authors.forename: M
authors.initials: M
authors.lastname: Gabriel
authors.forename: K
authors.initials: K
authors.lastname: Pawlaczyk
authors.forename: W
authors.initials: W
authors.lastname: Podstawski
mesh_terms: D000328:Adult; D000368:Aged; D000941:Antigens / Q000032:analysis*; D000951:Antigens, Neoplasm / Q000032:analysis; D001649:Bile Duct Diseases / Q000150:complications / Q000473:pathology*; D001650:Bile Duct Neoplasms / Q000150:complications / Q000473:pathology; D015415:Biomarkers / Q000032:analysis; D002760:Cholangiopancreatography, Endoscopic Retrograde; D003937:Diagnosis, Differential; D004848:Epithelium / Q000473:pathology; D005260:Female; D006801:Humans; D007124:Immunoenzyme Techniques; D007150:Immunohistochemistry; D007565:Jaundice / Q000209:etiology*; D008297:Male; D008875:Middle Aged; D011446:Prospective Studies; D012680:Sensitivity and Specificity
chemical_list: D000941:Antigens; D000951:Antigens, Neoplasm; D015415:Biomarkers
vernacular_title: Zastosowanie immunohistochemicznej analizy ekspresji antygenów oraz klasycznej cytologii złuszczonych komórek nabłonka dróg zółciowych w róznicowaniu przyczyn zółtaczki mechanicznej.