title: Molecular cloning and nucleotide sequence of the pyruvate kinase gene of an actinomycete Microbispora thermodiastatica.
abstract: The gene for the thermostable pyruvate kinase of Microbispora thermodiastatica IFO 14046, a moderate thermophilic actinomycete, was cloned in Escherichia coli. This gene consists of an open reading frame of 1422 nucleotides and encodes a protein of 474 amino acids with molecular mass of 50,805 Da. The open reading frame was confirmed as the pyruvate kinase gene by comparison with the N-terminal amino acid sequence of the purified pyruvate kinase from M. thermodiastatica.
authors.affiliation: Research and Development Division, Kikkoman Corporation, Chiba, Japan.
authors.forename: A
authors.initials: A
authors.lastname: Arai
authors.forename: S
authors.initials: S
authors.lastname: Masuda
authors.forename: A
authors.initials: A
authors.lastname: Matsuyama
authors.forename: S
authors.initials: S
authors.lastname: Murakami
authors.forename: M
authors.initials: M
authors.lastname: Nakajima
mesh_terms: D000191:Actinomycetaceae / Q000737:chemistry* / Q000201:enzymology / Q000235:genetics* / Q000254:growth & development; D001483:Base Sequence; D005798:Genes, Bacterial / Q000235:genetics*; D008969:Molecular Sequence Data; D011770:Pyruvate Kinase / Q000737:chemistry* / Q000235:genetics; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA
chemical_list: D011770:Pyruvate Kinase