title: Inhibitory effect of bupleuri radix saponins on adhesion of some solid tumor cells and relation to hemolytic action: screening of 232 herbal drugs for anti-cell adhesion.
abstract: Anti-cell adhesive activity and hemolytic action of herbal drugs were investigated. Among 232 herbal drugs tested, six showed a remarkable anti-cell adhesive activity, and the extract from the roots of Bupleurum falcatum (Umbelliferae), the semen of Psorala corylifolia (Leguminosae), and the semen of Areca catechu (Palmae) showed an anti-cell adhesive action at non-cytotoxic concentrations. Saikosaponins-a, d and e, isolated from the roots of Bupleurum falcatum, exhibited a potent anti-cell adhesive activity and a strong hemolytic action. In a structure-activity relationship for both activities, it seems that a sugar moiety and an ether linkage between C-13 and C-28 are required for good bioactivities. In addition, saikosaponin d with a beta-hydroxy group at C-16 was more potent than saikosaponin a possessing an alpha-hydroxy group. Taken together, it is suggested that the mechanism for anti-cell adhesive activity of saikosaponin may resemble that for their hemolytic action.
authors.affiliation: College of Pharmacy, Chungnam National University, Taejon, Korea. ahnbj@hanbat.chungnam.ac.kr
authors.forename: B Z
authors.initials: BZ
authors.lastname: Ahn
authors.forename: Y D
authors.initials: YD
authors.lastname: Yoon
authors.forename: Y H
authors.initials: YH
authors.lastname: Lee
authors.forename: B H
authors.initials: BH
authors.lastname: Kim
authors.forename: D E
authors.initials: DE
authors.lastname: Sok
mesh_terms: D000972:Antineoplastic Agents, Phytogenic / Q000494:pharmacology*; D002448:Cell Adhesion / Q000187:drug effects*; D004354:Drug Screening Assays, Antitumor; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D006461:Hemolysis / Q000187:drug effects*; D006801:Humans; D000432:Methanol; D010936:Plant Extracts / Q000494:pharmacology; D012503:Saponins / Q000494:pharmacology*; D014407:Tumor Cells, Cultured
chemical_list: D000972:Antineoplastic Agents, Phytogenic; D004365:Drugs, Chinese Herbal; D010936:Plant Extracts; D012503:Saponins; D000432:Methanol