title: SPIN, a substrate in the MAP kinase pathway in mouse oocytes.
abstract: The newly cloned gene Spin encodes a 30-kDa protein, a well-defined abundant molecule found in mouse oocytes and early embryos. This protein SPIN undergoes metaphase-specific phosphorylation and binds to the spindle. To understand the role of SPIN in oocyte meiosis, oocytes were treated with drugs that affect the cell cycle by activating or inactivating specific kinases. The posttranslational modification of SPIN in the treated oocytes was then investigated by one- and two-dimensional gel electrophoresis. Modification of SPIN is inhibited by treatment with 6-dimethylaminopurine (DMAP), suggesting that SPIN is phosphorylated by a serine-threonine kinase. Furthermore, SPIN from cycloheximide-treated oocytes that lack detectable MAP kinase activity is only partially phosphorylated, indicating that SPIN may be phosphorylated by the MOS/MAP kinase pathway. To confirm this observation, SPIN was analyzed in Mos-null mutant mice lacking MAP kinase activity. Normal posttranslational modification of SPIN did not occur in Mos-null mutant oocytes. In addition, there is reduced association of SPIN with the metaphase I spindle in Mos-null mutant oocytes, as determined by immunohistochemical analysis. These findings suggest that SPIN is a substrate in the MOS/ MAP kinase pathway and further that this phosphorylation of SPIN may be essential for its interaction with the spindle.
authors.affiliation: The Jackson Laboratory, Bar Harbor, Maine 04609, USA. bermoh@aretha.jax.org
authors.forename: B
authors.initials: B
authors.lastname: Oh
authors.forename: A
authors.initials: A
authors.lastname: Hampl
authors.forename: J J
authors.initials: JJ
authors.lastname: Eppig
authors.forename: D
authors.initials: D
authors.lastname: Solter
authors.forename: B B
authors.initials: BB
authors.lastname: Knowles
mesh_terms: D000225:Adenine / Q000031:analogs & derivatives / Q000494:pharmacology; D000818:Animals; D017871:Calcium-Calmodulin-Dependent Protein Kinases / Q000378:metabolism*; D018797:Cell Cycle Proteins / Q000378:metabolism*; D003513:Cycloheximide / Q000494:pharmacology; D004791:Enzyme Inhibitors / Q000494:pharmacology; D005260:Female; D051379:Mice; D008810:Mice, Inbred C57BL; D008869:Microtubule-Associated Proteins; D016296:Mutagenesis; D019319:Okadaic Acid / Q000494:pharmacology; D009865:Oocytes / Q000187:drug effects / Q000254:growth & development / Q000378:metabolism*; D010750:Phosphoproteins / Q000378:metabolism*; D011499:Protein Processing, Post-Translational; D017346:Protein Serine-Threonine Kinases / Q000037:antagonists & inhibitors*; D011500:Protein Synthesis Inhibitors / Q000494:pharmacology; D017231:Proto-Oncogene Proteins c-mos / Q000235:genetics / Q000378:metabolism*; D008941:Spindle Apparatus; D013379:Substrate Specificity
chemical_list: D018797:Cell Cycle Proteins; D004791:Enzyme Inhibitors; D008869:Microtubule-Associated Proteins; D010750:Phosphoproteins; D011500:Protein Synthesis Inhibitors; C104434:spindlin; D019319:Okadaic Acid; C001020:N(6),N(6)-dimethyladenine; D003513:Cycloheximide; D017346:Protein Serine-Threonine Kinases; D017231:Proto-Oncogene Proteins c-mos; D017871:Calcium-Calmodulin-Dependent Protein Kinases; D000225:Adenine