Specific interaction between 5'-aminonucleosides and borates and influence of borates on nucleoside conformations.
The unique conformation and orientational change of 5'-amino-5'-deoxyuridine in Kolthoff's buffer is discussed. From CD and NOE spectra, anomalous syn orientation of Urd-5'-NH2 might be caused by specific interaction between borates and Urd-5'-NH2, which promote the formation of hydrogen bonding between 2-carbonyl oxygen and hydrogen of 5'-amino group. This borates induced syn orientation of Urd-5'-NH2 could be applied to external stimulation control of target RNA/DNA hybridization.
Wada
T
T
Faculty of Engineering, Osaka University, Japan.
Minamimoto
N
N
Inaki
Y
Y
Inoue
Y
Y
D001881:Borates / Q000737:chemistry*; D002021:Buffers; D002942:Circular Dichroism; D004247:DNA / Q000737:chemistry; D003857:Deoxyuridine / Q000031:analogs & derivatives* / Q000737:chemistry; D006860:Hydrogen Bonding; D007202:Indicators and Reagents; D008968:Molecular Conformation; D015394:Molecular Structure; D019906:Nuclear Magnetic Resonance, Biomolecular; D009693:Nucleic Acid Hybridization; D012313:RNA / Q000737:chemistry
C113077:5'-amino-5'-deoxyuridine; D001881:Borates; D002021:Buffers; D007202:Indicators and Reagents; D012313:RNA; D004247:DNA; D003857:Deoxyuridine