Influence of dietary zinc on convulsive seizures and hippocampal NADPH diaphorase-positive neurons in seizure susceptible EL mouse.
Adequate, high and deficient dietary levels of zinc (Zn) were compared in seizure-susceptible EL mice with respect to convulsions and to nicotinamide adenine dinucleotide phosphate (NADPH) diaphorase-positive hippocampal neurons. Diaphorase positivity is associated with nitric oxide (NO) production. Convulsive seizures in the EL mice given the various diets did not differ over 1-4 weeks, but convulsions in EL mice given the Zn-deficient diet for 4 weeks were more effectively suppressed by injection of zonisamide (ZNS) (75 mg/kg intraperitoneally) than in mice receiving high- or adequate-Zn diet for the same period. Numbers of NADPH diaphorase-positive neurons in the CA1/CA2 region of the hippocampal formation were significantly higher in mice given the Zn-deficient diet for 4 weeks than in mice fed adequate Zn. Mice receiving the high-Zn diet for the same period had significantly fewer NADPH diaphorase-positive neurons in the subiculum than mice with adequate Zn. These results suggest that Zn deficiency inhibits convulsive seizures of EL mice, and that dietary Zn influences numbers of NO producing neurons in the hippocampal formation.
Nagatomo
I
I
Department of Neuropsychiatry, Faculty of Medicine, Kagoshima University, 8-35-1, Sakuragaoka, Kagoshima 890, Japan.
Akasaki
Y
Y
Uchida
M
M
Kuchiiwa
S
S
Nakagawa
S
S
Takigawa
M
M
D000818:Animals; D000927:Anticonvulsants / Q000494:pharmacology; D002452:Cell Count / Q000187:drug effects; D004032:Diet; D020022:Genetic Predisposition to Disease; D006624:Hippocampus / Q000201:enzymology* / Q000473:pathology; D007555:Isoxazoles / Q000494:pharmacology; D051379:Mice; D008817:Mice, Mutant Strains / Q000235:genetics; D009252:NADPH Dehydrogenase / Q000378:metabolism*; D009474:Neurons / Q000187:drug effects / Q000201:enzymology* / Q000473:pathology; D012640:Seizures / Q000201:enzymology / Q000235:genetics* / Q000503:physiopathology*; D015032:Zinc / Q000008:administration & dosage* / Q000172:deficiency / Q000494:pharmacology; D000078305:Zonisamide
D000927:Anticonvulsants; D007555:Isoxazoles; D000078305:Zonisamide; D009252:NADPH Dehydrogenase; D015032:Zinc