title: Regulation of distal nephron K+ channels (ROMK) mRNA expression by aldosterone in rat kidney.
abstract: 1. The expression of ROMK mRNA isoforms in rat kidney was measured using competitive polymerase chain reaction. Under basal conditions the expression of ROMK2 and 3 mRNA was significantly higher than that of ROMK1 or 6. 2. Administration of aldosterone for a period of 1 week significantly increased the mRNA expression not only of the alpha1-subunit of the Na+-K+-ATPase, but also of ROMK2, 3 and 6. 3. These data not only provide evidence that ROMK K+ channels may be involved with mineralocorticoid-sensitive K+ secretion in the distal nephron, but also demonstrate for the first time that ROMK6 may be involved in this process.
authors.affiliation: Laboratory for Membrane Protein Function, Department of Biomedical Science, University of Sheffield, Western Bank, Sheffield S10 2TN, UK.
authors.forename: A H
authors.initials: AH
authors.lastname: Beesley
authors.forename: D
authors.initials: D
authors.lastname: Hornby
authors.forename: S J
authors.initials: SJ
authors.lastname: White
mesh_terms: D000199:Actins / Q000235:genetics; D000450:Aldosterone / Q000097:blood / Q000494:pharmacology*; D000818:Animals; D017931:DNA Primers; D015870:Gene Expression / Q000187:drug effects / Q000502:physiology; D007536:Isomerism; D008297:Male; D009399:Nephrons / Q000737:chemistry* / Q000201:enzymology; D016133:Polymerase Chain Reaction; D015221:Potassium Channels / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism*; D024661:Potassium Channels, Inwardly Rectifying; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D000254:Sodium-Potassium-Exchanging ATPase / Q000235:genetics / Q000378:metabolism
chemical_list: D000199:Actins; D017931:DNA Primers; C510692:Kcnj1 protein, rat; D015221:Potassium Channels; D024661:Potassium Channels, Inwardly Rectifying; D012333:RNA, Messenger; D000450:Aldosterone; D000254:Sodium-Potassium-Exchanging ATPase