Proliferative effect of phospholipase A2 on rat periacinar fibroblastoid cells of the pancreas.
We have shown previously that rat pancreatic periacinar fibroblastoid cells (PFCs) can be cultured from isolated pancreatic acini. In the present study, immunocytochemical examination of the PFC extracellular matrix was performed using antibodies against prolyl hydroxylase alpha and beta subunits, types I, III, and IV collagen, fibronectin, and laminin. The PFC content of alpha-smooth muscle actin and platelet-derived growth factor (PDGF) receptor were studied by immunoblotting. We demonstrated that PFCs synthesized extracellular matrix and expressed alpha-smooth muscle actin and PDGF receptors. These results suggested that PFCs resemble myofibroblasts and may play a critical role in pancreatic fibrosis. Conversely, pancreatic-type phospholipase A2 (P-PLA2), one of the pancreatic digestive enzymes, has been shown to induce DNA synthesis of Swiss 3T3 fibroblasts. To determine whether this enzyme is involved in pancreatic fibrosis, we studied P-PLA2's proliferative and chemotactic effects on PFCs as well as its digestive activity. The proliferative and chemotactic effects were investigated using 3H-thymidine incorporation and a chemotactic assay, respectively. P-PLA2 had both proliferative and chemotactic effects. P-PLA2 is considered a growth factor for PFCs and is implicated in pancreatic fibrosis.
Okumura
Y
Y
Second Department of Internal Medicine, Shiga University of Medical Science, Otsu, Japan.
Shintani
Y
Y
Kato
Y
Y
Tamba
J
J
Inoue
H
H
Fujiyama
Y
Y
Bamba
T
T
D000199:Actins / Q000032:analysis; D000818:Animals; D002478:Cells, Cultured; D004247:DNA / Q000096:biosynthesis; D004305:Dose-Response Relationship, Drug; D016326:Extracellular Matrix Proteins / Q000032:analysis; D005355:Fibrosis; D008297:Male; D010179:Pancreas / Q000737:chemistry / Q000187:drug effects* / Q000473:pathology; D010741:Phospholipases A / Q000494:pharmacology*; D054467:Phospholipases A2; D051381:Rats; D017208:Rats, Wistar
D000199:Actins; D016326:Extracellular Matrix Proteins; D004247:DNA; D010741:Phospholipases A; D054467:Phospholipases A2