title: The role of endothelin-1 in regulation of rat mesenteric microcirculation.
abstract: Immunocytochemistry of endothelin-1 (ET-1) and the ETA receptor, and scanning electron microscopy using cast resin after treatment with ET-1, were carried out in the rat mesenteric microvasculature. Immunoreaction of ET-1 was preferentially seen along the endothelium of the proximal portion of the anterior mesenteric artery, the endothelial cells of which contain abundant Weibel-Palade (WP) bodies. However, the arterioles and small veins distal to the artery showed little immunoreactivity but showed heavy immunoreaction of ETA receptors in the media. By scanning electron microscopy after treatment with ET-1, the mesenteric microvasculature became slightly narrower compared to the control exhibited of localized constricted areas, especially in the region of the small veins. Light microscopy of such areas revealed localized thickening of the medial muscle cells. Because the release of ET-1 from endothelial cells depends in part on extracellular discharge of the WP bodies, the results indicate that ET-1, discharged from the proximal portion of the anterior mesenteric artery, induces vasoconstriction of the arterioles and small veins, mediated by ETA receptors. The localized thickening in areas in the media of the small veins may participate in the maintenance of blood flow through the portal circulation.
authors.affiliation: Department of Anatomy, University of Occupational and Environmental Health, School of Medicine, Kitakyusyu, Japan.
authors.forename: K
authors.initials: K
authors.lastname: Kayashima
authors.forename: H
authors.initials: H
authors.lastname: Kudo
authors.forename: Y
authors.initials: Y
authors.lastname: Doi
authors.forename: S
authors.initials: S
authors.lastname: Fujimoto
mesh_terms: D000818:Animals; D019332:Endothelin-1 / Q000502:physiology*; D007150:Immunohistochemistry; D008297:Male; D017538:Mesenteric Artery, Superior / Q000033:anatomy & histology / Q000187:drug effects / Q000648:ultrastructure; D008854:Microscopy, Electron; D008855:Microscopy, Electron, Scanning; D051381:Rats; D017208:Rats, Wistar; D013152:Splanchnic Circulation / Q000502:physiology*
chemical_list: D019332:Endothelin-1