Modified nucleic acids for in vitro selection.
Some types of modified nucleic acid were tested for in vitro evolution or systematic evolution of ligands by exponential enrichment (SELEX). T7 RNA polymerase accepted cytidine triphosphate carrying biotinyl group at the N4-position of cytosine, adenosine triphosphate carrying aminohexyl group at the N6-position of adenine, and 2'-amino-2'-deoxyuridine triphosphate as substrates for transcription of DNA containing random sequence, but not uridine triphosphate carrying a nitroveratryloxy group at the 2'-position nor adenosine triphosphate carrying aminohexyl group at the 8-position of adenine. The transcribed products served as templates for cDNA by reverse transcription. These findings indicate that some modified nucleotides can be applied to the present in vitro selection.
Ito
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Graduate School of Material Science, Kyoto, Japan.
D018076:DNA, Complementary; D012321:DNA-Directed RNA Polymerases / Q000378:metabolism*; D003854:Deoxyribonucleotides / Q000378:metabolism*; D007256:Information Systems; D016133:Polymerase Chain Reaction / Q000379:methods*; D012265:Ribonucleotides / Q000378:metabolism*; D013379:Substrate Specificity; D013698:Templates, Genetic; D014158:Transcription, Genetic; D014764:Viral Proteins
D018076:DNA, Complementary; D003854:Deoxyribonucleotides; D012265:Ribonucleotides; D014764:Viral Proteins; C062210:bacteriophage T7 RNA polymerase; D012321:DNA-Directed RNA Polymerases