Changes in endothelin-1, 6-keto-PG-F1 alpha, and TX-B2 in random pattern flaps.
In this study we investigated kinetic changes in the ratio of endothelin-1 (ET-1) and eicosanoids (6-keto-PG-F1 alpha TX-B2) in 20 x 60 mm random pattern flaps from rats. The ET-1 content of regions A (20 mm from the peripheral end) and B (20-40 mm) 6 h after surgery tended to decrease slightly compared to the ET-1 content immediately after surgery. The ET-1 content of region C (20 mm from the flap base) 6 h postoperatively increased significantly compared to that immediately after surgery. The ET-1 content of region C 6 h after surgery was significantly higher compared to that of regions A and B, which were obtained simultaneously. The ratios of eicosanoids in the three regions 6 h after surgery were significantly lower than those immediately after operation. However, the ratio in region A was higher than that in region C, showing that there was a difference in distribution in the flap between ET-1 and eicosanoids. The administration of an ETA receptor antagonist, FR-139317, extended the survival length of the flap. These results suggest that ET-1 can regulate the microcirculation in a flap directly and/or indirectly.
Inoue
H
H
Department of Plastic and Reconstructive Surgery, St. Marianna University School of Medicine, Kawasaki, Japan.
Aihara
M
M
Tomioka
M
M
Ishida
H
H
D015121:6-Ketoprostaglandin F1 alpha / Q000378:metabolism*; D000818:Animals; D001381:Azepines / Q000494:pharmacology; D015777:Eicosanoids / Q000378:metabolism; D065128:Endothelin Receptor Antagonists; D019332:Endothelin-1 / Q000378:metabolism*; D007211:Indoles / Q000494:pharmacology; D008297:Male; D009336:Necrosis; D051381:Rats; D017208:Rats, Wistar; D044022:Receptor, Endothelin A; D012867:Skin / Q000378:metabolism* / Q000473:pathology; D013929:Thromboxane B2 / Q000378:metabolism*
D001381:Azepines; D015777:Eicosanoids; D065128:Endothelin Receptor Antagonists; D019332:Endothelin-1; D007211:Indoles; D044022:Receptor, Endothelin A; C079574:FR 139317; D013929:Thromboxane B2; D015121:6-Ketoprostaglandin F1 alpha