Expression of parathyroid hormone/parathyroid hormone-related protein receptor in vascular endothelial cells.
The parathyroid hormone (PTH)/PTH-related protein (PTHrP) receptor has been reported to be expressed in many tissues, including vascular smooth-muscle cells (VSMCs), but it has not been identified in vascular endothelial cells. To determine whether vascular endothelial cells can express the PTH/PTHrP receptor, its gene expression was examined in simian virus 40-transformed rat lung vascular endothelial cells (TRLECs) by the reverse-transcription polymerase chain reaction (RT-PCR). Results in TRLEC, with rat VSMCs and kidney as controls, showed identical 741-bp products. Furthermore, incubation with PTHrP[1-34] reduced the thrombin-stimulated endothelin-1 (ET-1) expression in TRLECs. Our results demonstrate that vascular endothelial cells can express the PTH/PTHrP receptor and therefore are also a target tissue for PTHrP.
Jiang
B
B
Department of Geriatric Medicine, Osaka University Medical School, Japan.
Morimoto
S
S
Yang
J
J
Niinoabu
T
T
Fukuo
K
K
Ogihara
T
T
D000818:Animals; D015152:Blotting, Northern; D004730:Endothelium, Vascular / Q000166:cytology / Q000378:metabolism*; D066298:In Vitro Techniques; D008168:Lung / Q000378:metabolism; D010281:Parathyroid Hormone / Q000096:biosynthesis*; D044162:Parathyroid Hormone-Related Protein; D016133:Polymerase Chain Reaction; D014176:Protein Biosynthesis; D012313:RNA / Q000096:biosynthesis; D051381:Rats; D013539:Simian virus 40 / Q000235:genetics
D010281:Parathyroid Hormone; D044162:Parathyroid Hormone-Related Protein; D012313:RNA