Evolution of a phosphorothioate RNA library during in vitro selection.
We carried out an in vitro selection of aptamers from an RNA library with phosphorothioate linkages instead of normal phosphodiesters. This "thio-RNA" library had smaller dissociation rate constant (kd) to a target protein than a natural RNA library. On the basis of the results, an improved selection procedure to obtain ideal nucleic acid drugs will be discussed.
Kawakami
J
J
Department of Chemistry, Faculty of Science, Konan University, Kobe, Japan.
Sugimoto
N
N
D001483:Base Sequence; D004247:DNA / Q000737:chemistry; D015696:Gene Products, tat / Q000096:biosynthesis / Q000235:genetics; D015497:HIV-1 / Q000235:genetics; D016376:Oligonucleotides, Antisense; D016133:Polymerase Chain Reaction; D012313:RNA / Q000737:chemistry*; D013873:Thionucleotides; D054322:tat Gene Products, Human Immunodeficiency Virus
D015696:Gene Products, tat; D016376:Oligonucleotides, Antisense; D013873:Thionucleotides; D054322:tat Gene Products, Human Immunodeficiency Virus; D012313:RNA; D004247:DNA