title: In vivo EPR detection and imaging of endogenous nitric oxide in lipopolysaccharide-treated mice.
abstract: Nitric oxide (NO), a simple diatomic free radical, is known to play a critical physiological role in diverse organisms. An iron complex, with N-(dithiocarboxy)sarcosine (Fe-DTCS), has a high affinity for endogenous NO and can trap, stabilize, and accumulate it. The stable NO adduct thus formed is detectable at room temperature with electron paramagnetic resonance (EPR) spectrometry. We report in vivo EPR imaging of endogenous NO, trapped by an Fe-DTCS complex, in the abdomen of a live mouse. To our knowledge, this is the first report on EPR imaging of endogenous free radicals produced in vivo. This EPR imaging method will be useful for the noninvasive investigation of the spatial distribution of NO in pathologic organs or tissues.
authors.affiliation: Institute for Life Support Technology, Yamagata Technopolis Foundation, Japan. yoshi@ymgt-techno.or.jp
authors.forename: T
authors.initials: T
authors.lastname: Yoshimura
authors.forename: H
authors.initials: H
authors.lastname: Yokoyama
authors.forename: S
authors.initials: S
authors.lastname: Fujii
authors.forename: F
authors.initials: F
authors.lastname: Takayama
authors.forename: K
authors.initials: K
authors.lastname: Oikawa
authors.forename: H
authors.initials: H
authors.lastname: Kamada
mesh_terms: D000818:Animals; D004578:Electron Spin Resonance Spectroscopy; D005260:Female; D008070:Lipopolysaccharides / Q000494:pharmacology*; D051379:Mice; D008813:Mice, Inbred ICR; D009569:Nitric Oxide / Q000032:analysis*; D012521:Sarcosine / Q000031:analogs & derivatives / Q000737:chemistry; D013113:Spin Labels; D013859:Thiocarbamates / Q000737:chemistry
chemical_list: D008070:Lipopolysaccharides; D013113:Spin Labels; D013859:Thiocarbamates; D009569:Nitric Oxide; C055171:sarcosine dithiocarbamate; D012521:Sarcosine