title: Sequence of the Octopus dofleini hemocyanin subunit: structural and evolutionary implications.
abstract: Sequencing of the subunit of the hemocyanin of Octopus dofleini has been completed from a cDNA library. This represents the first molluscan hemocyanin to be completely sequenced. The sequence determined is for one of the two distinguishable cDNAs which have been recognized for this protein. The protein subunit has 2896 amino acids and contains seven functional units, each carrying two sets of three invariant histidine residues constituting the binding sites (A and B) for two copper atoms. The accompanying paper identifies this site in the C-terminal functional unit (Odg). Differences in sequence for the two cDNAs, for the region in which both are available, are concentrated in the "linker regions" between functional units. The sequences of the seven units exhibit high similarity, averaging about 40% identity, with a concentration of conserved sequences in the region surrounding the copper binding sites. The sequences around the B-site show significant homology to the sequences of arthropod hemocyanins. Comparison of the functional unit sequences in terms of hydrophobicity and surface exposure profiles, as well as regions of probable secondary structure, indicate that all functional units probably have a common tertiary folding; the protein subunit is a string of similarly folded beads. A number of putative N-linked carbohydrate binding sites can be recognized in the sequence; one of these corresponds to the carbohydrate observed in the X-ray diffraction study of functional unit Odg as disclosed in the accompying paper. Phylogenetic analysis of the sequences of the O. dofleini functional units, and comparison with other available molluscan sequences indicates that the multi-domain subunit structure must have arisen over a relatively brief period, preceeding the differentiation of major molluscan types.
authors.affiliation: Department of Biochemistry and Biophysics, Oregon State University Corvallis 97331, USA.
authors.forename: K I
authors.initials: KI
authors.lastname: Miller
authors.forename: M E
authors.initials: ME
authors.lastname: Cuff
authors.forename: W F
authors.initials: WF
authors.lastname: Lang
authors.forename: P
authors.initials: P
authors.lastname: Varga-Weisz
authors.forename: K G
authors.initials: KG
authors.lastname: Field
authors.forename: K E
authors.initials: KE
authors.lastname: van Holde
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D002240:Carbohydrate Sequence; D003300:Copper; D018076:DNA, Complementary / Q000235:genetics; D019281:Dimerization; D019143:Evolution, Molecular; D015723:Gene Library; D006023:Glycoproteins / Q000737:chemistry / Q000235:genetics; D006433:Hemocyanins / Q000737:chemistry* / Q000235:genetics*; D008958:Models, Molecular; D008969:Molecular Sequence Data; D008974:Mollusca / Q000737:chemistry / Q000235:genetics; D049831:Octopodiformes / Q000235:genetics*; D010802:Phylogeny; D017433:Protein Structure, Secondary; D017434:Protein Structure, Tertiary; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D012996:Solutions; D013499:Surface Properties
chemical_list: D018076:DNA, Complementary; D006023:Glycoproteins; D012996:Solutions; D003300:Copper; D006433:Hemocyanins