Cloning, characterization, and expression of the CIP2 gene induced under cadmium stress in Candida sp.
We isolated, sequenced, and expressed a cadmium-induced gene, CIP2, that specifically hybridizes to a mRNA of approximately 950 nucleotides. The CIP2 mRNA was barely present in normal Candida cells, but accumulated at higher levels in cadmium-treated cells. Other heavy metals such as copper, mercury, lead, and manganese had no effect on the expression of the CIP2 gene. CIP2 contains an open reading frame encoding a protein of 203 amino acids. This gene was also activated by an oxidant, diamide. Our results suggest that CIP2 may play a crucial role in the specific cellular response to oxidative stress evolved by the cadmium treatment.
Park
K S
KS
Department of Microbiology, HanNam University, Taeduk-Ku, Taejon, South Korea.
Kwon
J
J
Choi
S Y
SY
D000595:Amino Acid Sequence; D001483:Base Sequence; D015152:Blotting, Northern; D002104:Cadmium / Q000633:toxicity*; D002175:Candida / Q000187:drug effects* / Q000235:genetics; D005800:Genes, Fungal / Q000187:drug effects* / Q000235:genetics; D008969:Molecular Sequence Data
D002104:Cadmium