title: Gammadelta T cells present antigen to CD4+ alphabeta T cells.
abstract: Gammadelta and alphabeta TCR+ T cells share many properties and their interactions are likely to be coordinated and regulated. We provide evidence that cattle gammadelta T cells are able to present antigen to CD4+ T cells. To help elucidate their function gammadelta T cell lines were propagated for extensive characterization. Cells expressed high levels of MHC class II and production of co-stimulatory molecules as evidenced by the binding of a CTLA-4 fusion protein and synthesis of CD80 transcripts. These properties and the presence of a well-developed endosomal compartment indicated the cells might function as antigen-presenting cells. Resting CD4+ T cells from calves immunized with ovalbumin or respiratory syncytial virus-antigen proliferated in response to gammadelta T cells pulsed with antigen that appeared to be endocytosed via clathrin-coated pits or specific receptors.
authors.affiliation: Division of Immunology and Pathology, Institute for Animal Health, Newbury, Berks, United Kingdom. COLLINS@BBSRC.AC.UK
authors.forename: R A
authors.initials: RA
authors.lastname: Collins
authors.forename: D
authors.initials: D
authors.lastname: Werling
authors.forename: S E
authors.initials: SE
authors.lastname: Duggan
authors.forename: A P
authors.initials: AP
authors.lastname: Bland
authors.forename: K R
authors.initials: KR
authors.lastname: Parsons
authors.forename: C J
authors.initials: CJ
authors.lastname: Howard
mesh_terms: D000069594:Abatacept; D000818:Animals; D017951:Antigen Presentation / Q000502:physiology*; D000938:Antigen-Presenting Cells / Q000276:immunology* / Q000378:metabolism; D015703:Antigens, CD; D000943:Antigens, Differentiation / Q000378:metabolism; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology* / Q000378:metabolism; D060908:CTLA-4 Antigen; D002417:Cattle; D002478:Cells, Cultured; D016207:Cytokines / Q000096:biosynthesis; D016650:Fluorescein-5-isothiocyanate / Q000493:pharmacokinetics; D000949:Histocompatibility Antigens Class II / Q000096:biosynthesis; D018796:Immunoconjugates; D007156:Immunologic Memory / Q000502:physiology; D007376:Interleukin-2 / Q000494:pharmacology; D008213:Lymphocyte Activation / Q000502:physiology; D051379:Mice; D010047:Ovalbumin / Q000493:pharmacokinetics; D010641:Phenotype; D012333:RNA, Messenger / Q000378:metabolism; D016693:Receptors, Antigen, T-Cell, alpha-beta / Q000276:immunology*; D016692:Receptors, Antigen, T-Cell, gamma-delta / Q000276:immunology*; D013601:T-Lymphocytes / Q000276:immunology* / Q000378:metabolism
chemical_list: D015703:Antigens, CD; D000943:Antigens, Differentiation; D060908:CTLA-4 Antigen; C556707:Ctla4 protein, mouse; D016207:Cytokines; D000949:Histocompatibility Antigens Class II; D018796:Immunoconjugates; D007376:Interleukin-2; D012333:RNA, Messenger; D016693:Receptors, Antigen, T-Cell, alpha-beta; D016692:Receptors, Antigen, T-Cell, gamma-delta; D000069594:Abatacept; D010047:Ovalbumin; D016650:Fluorescein-5-isothiocyanate