title: The production of D-acetoin by a transgenic Escherichia coli.
abstract: A 6 kbp SphI fragment encoding genes for the enzymes alpha-acetolactate synthase (ALS), alpha-acetolactate decarboxylase (ALDC) and meso-2,3-butanediol dehydrogenase (meso-BDH), involved in the formation of meso-2,3-butanediol (meso-BD) from pyruvic acid, was cloned into plasmid pUC118. When derivatives of this plasmid were introduced into Escherichia coli JM109, the transformants were able to produce D-acetoin (D-AC) without contamination with L-acetoin (L-AC).
authors.affiliation: Department of Applied Chemistry and Biotechnology, Faculty of Engineering, Yamanashi University, Japan.
authors.forename: S
authors.initials: S
authors.lastname: Ui
authors.forename: A
authors.initials: A
authors.lastname: Mimura
authors.forename: M
authors.initials: M
authors.lastname: Okuma
authors.forename: T
authors.initials: T
authors.lastname: Kudo
mesh_terms: D000093:Acetoin / Q000378:metabolism*; D000095:Acetolactate Synthase / Q000235:genetics; D000429:Alcohol Oxidoreductases / Q000235:genetics; D002262:Carboxy-Lyases / Q000235:genetics; D002849:Chromatography, Gas; D003470:Culture Media / Q000737:chemistry; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism*; D013997:Time Factors; D014169:Transformation, Bacterial
chemical_list: D003470:Culture Media; D000093:Acetoin; D000429:Alcohol Oxidoreductases; C019801:butanediol dehydrogenase; D000095:Acetolactate Synthase; D002262:Carboxy-Lyases; C023746:acetolactate decarboxylase