title: Thermophilic strain Bacillus species AA contains several site-specific endonucleases.
abstract: Thermophilic strain Bacillus species AA contains several site-specific endonucleases and three of them have been identified. BspAAI recognizes the sequence 5'-C downward arrow TCGAG-3' and cleaves it after the first "C" forming 4-nucleotide 5'-ends and is an isoschizomer of XhoI. BspAAII recognizes the sequence 5'-T downward arrow CTAGA-3' and cleaves it after the first "T" forming 4-nucleotide 5'-ends and is an isoschizomer of XbaI. BspAAIII recognizes the sequence 5'-GGATCC-3' and is an isomer of BamHI. The optimal temperature and pH values are 42-48 degreesC and 7.0-8.0, respectively.
authors.affiliation: Institute of Protein Research, Russian Academy of Sciences, Pushchino, Moscow Region, 142292, Russia. nikmatv@sun.ipr.serpukhov.su
authors.forename: D T
authors.initials: DT
authors.lastname: Alzhanov
authors.forename: D V
authors.initials: DV
authors.lastname: Alzhanova
authors.forename: L A
authors.initials: LA
authors.lastname: Zheleznaya
authors.forename: N I
authors.initials: NI
authors.lastname: Matvienko
mesh_terms: D001407:Bacillus / Q000201:enzymology*; D010582:Bacteriophage lambda / Q000235:genetics / Q000378:metabolism; D001483:Base Sequence; D001665:Binding Sites; D004279:DNA, Viral / Q000235:genetics / Q000378:metabolism; D015245:Deoxyribonuclease BamHI / Q000302:isolation & purification / Q000378:metabolism; D015252:Deoxyribonucleases, Type II Site-Specific / Q000302:isolation & purification / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D013379:Substrate Specificity; D013696:Temperature
chemical_list: D004279:DNA, Viral; D015245:Deoxyribonuclease BamHI; C025580:endodeoxyribonuclease XBAI; C034510:CTCGAG-specific type II deoxyribonucleases; D015252:Deoxyribonucleases, Type II Site-Specific