Successful expression of a selenomethionyl protein under control of the temperature-sensitive lambda repressor requires higher than normal temperature.
Witkowski
A
A
Children's Hospital Oakland Research Institute, CA 94609-1809, USA.
Smith
S
S
D010582:Bacteriophage lambda / Q000235:genetics; D004268:DNA-Binding Proteins; D004926:Escherichia coli / Q000235:genetics; D064429:Fatty Acid Synthases / Q000235:genetics; D015964:Gene Expression Regulation, Bacterial; D006358:Hot Temperature; D011994:Recombinant Proteins / Q000096:biosynthesis* / Q000235:genetics*; D012097:Repressor Proteins / Q000235:genetics*; D012645:Selenomethionine / Q000378:metabolism*; D013869:Thiolester Hydrolases / Q000235:genetics; D014764:Viral Proteins; D054334:Viral Regulatory and Accessory Proteins
D004268:DNA-Binding Proteins; D011994:Recombinant Proteins; D012097:Repressor Proteins; D014764:Viral Proteins; D054334:Viral Regulatory and Accessory Proteins; C016649:phage repressor proteins; D012645:Selenomethionine; D064429:Fatty Acid Synthases; D013869:Thiolester Hydrolases; C022738:thioesterase II