Cholecystokinin satiety involves CCKA receptors perfused by the superior pancreaticoduodenal artery.
Three experiments compared the potency of the type A cholecystokinin (CCKA)-receptor antagonist devazepide for increasing intake of 30% sucrose when injected into the superior pancreaticoduodenal (SPD) artery (SPD group) or jugular vein (IV group). In experiment 1, 15 min of sucrose intake in adult, male Sprague-Dawley rats after 6 h of food deprivation was increased by devazepide (20 micrograms/kg) administered into the SPD artery whether given alone or in conjunction with cholecystokinin octapeptide (CCK-8, 2 micrograms/kg ip). Devazepide had no effect in the IV group. In experiment 2, injection of 8, 20, and 50 micrograms/kg of devazepide into the SPD artery increased sucrose intake of nondeprived rats. Only the highest dose was effective in the IV group. On subsequent tests, administration of 1 microgram/kg of CCK-8 significantly suppressed intake only in the SPD group. In experiment 3, nondeprived rats with SPD artery and jugular vein catheters were tested in a within-subjects design. Devazepide (20 micrograms/kg) increased sucrose intake after injection into the SPD artery, but not into the jugular vein. In experiment 4, intraduodenal devazepide (8, 20, and 50 micrograms/kg) had no effect. These results indicate that CCKA receptors within the SPD arterial bed mediate the satiating action of CCK, consistent with local action of duodenal CCK.
Cox
J E
JE
Department of Psychology, University of Alabama at Birmingham 35294, USA.
D000818:Animals; D001570:Benzodiazepinones / Q000008:administration & dosage; D002766:Cholecystokinin / Q000378:metabolism*; D020109:Devazepide; D004386:Duodenum / Q000098:blood supply / Q000378:metabolism*; D006727:Hormone Antagonists / Q000008:administration & dosage; D008297:Male; D010477:Perfusion; D051381:Rats; D017207:Rats, Sprague-Dawley; D011949:Receptors, Cholecystokinin / Q000037:antagonists & inhibitors / Q000378:metabolism*; D013395:Sucrose / Q000378:metabolism*
D001570:Benzodiazepinones; D006727:Hormone Antagonists; D011949:Receptors, Cholecystokinin; D013395:Sucrose; D002766:Cholecystokinin; D020109:Devazepide