title: Regeneration of the rat sciatic nerve into allografts made acellular through chemical extraction.
abstract: The aim of this study was to develop a procedure by which myelin and Schwann cells could be removed from a peripheral nerve while the basal lamina tubes, remained intact, and to test if such preparations could be used as allografts for the repair of a gap in the continuity of the rat sciatic nerve. We found that extraction with the detergents Triton X-100 and deoxycholate resulted in acellular nerve segments with preserved basal lamina tubes, here defined as the tubes which surrounds the axon/Schwann cell units. The morphology of the acellular nerve segments was revealed by scanning electron microscopy, teasing, immunohistochemistry and electrophoresis. Such grafts when allografted between two outbred rat strains, were found to support outgrowth of axons and migration of Schwann cells, which reoccupied the empty basal lamina tubes without excessive signs of inflammation. This new paradigm offers a possible solution to the major shortcomings of autologous nerve grafts, i.e., the requirement to sacrifice a healthy nerve and the shortage of graft material available for repair.
authors.affiliation: Department of Animal Physiology, University of Lund, Helgonav. 3 B, S-223 62 Lund, Sweden.
authors.forename: M
authors.initials: M
authors.lastname: Sondell
authors.forename: G
authors.initials: G
authors.lastname: Lundborg
authors.forename: M
authors.initials: M
authors.lastname: Kanje
mesh_terms: D000818:Animals; D001369:Axons / Q000502:physiology / Q000648:ultrastructure; D001485:Basement Membrane / Q000737:chemistry; D002196:Capillaries / Q000502:physiology; D002469:Cell Separation / Q000379:methods; D003094:Collagen; D003840:Deoxycholic Acid; D004210:Dissection; D005260:Female; D005904:Glial Fibrillary Acidic Protein / Q000032:analysis; D008264:Macrophages / Q000166:cytology; D008855:Microscopy, Electron, Scanning; D009186:Myelin Sheath / Q000737:chemistry / Q000637:transplantation* / Q000648:ultrastructure; D009416:Nerve Regeneration / Q000502:physiology*; D009419:Nerve Tissue Proteins / Q000032:analysis; D017830:Octoxynol; D010525:Peripheral Nerves / Q000098:blood supply / Q000737:chemistry; D051381:Rats; D017207:Rats, Sprague-Dawley; D017208:Rats, Wistar; D009418:S100 Proteins / Q000032:analysis; D012583:Schwann Cells / Q000737:chemistry / Q000637:transplantation* / Q000648:ultrastructure; D012584:Sciatic Nerve / Q000098:blood supply / Q000502:physiology* / Q000601:surgery; D014184:Transplantation, Homologous
chemical_list: D005904:Glial Fibrillary Acidic Protein; D009419:Nerve Tissue Proteins; D009418:S100 Proteins; D003840:Deoxycholic Acid; D017830:Octoxynol; D003094:Collagen