title: Evidence for oxidatively modified lipid-protein complexes in endometrium and endometriosis.
abstract: OBJECTIVE
  To evaluate for the presence of oxidatively modified lipid-protein complexes in endometriosis and endometrium of women with endometriosis and controls.
  
  
  METHODS
  Controlled clinical study.
  
  
  METHODS
  Academic tertiary care center.
  
  
  METHODS
  Women undergoing surgery for pelvic pain, infertility, endometriosis, or tubal ligation controls.
  
  
  METHODS
  Biopsy of endometrium and endometriosis.
  
  
  METHODS
  Staining with antibodies to oxidatively modified lipid proteins (HNE-7, MDA2), macrophages (HAM-56), and muscle cell actin (HHF-35).
  
  
  RESULTS
  Both endometrium and endometriosis tissues contain stromal cells that immunostain with HAM-56 and show immunostaining (both intracellular and extracellular) with HNE-7 and MDA2. Some endometriotic implants show patchy staining with HHF-35. Endometrium was devoid of staining with HHF-35. Control staining with nonimmune sera in both tissues was also devoid of staining.
  
  
  CONCLUSIONS
  These data strongly implicate the occurrence of oxidative stress in endometriosis tissue. These data also suggest that oxidative modification is a normal physiological process in endometrium.
authors.affiliation: Department of Gynecology and Obstetrics, Emory University, Atlanta, Georgia 30322, USA.
authors.forename: A A
authors.initials: AA
authors.lastname: Murphy
authors.forename: W
authors.initials: W
authors.lastname: Palinski
authors.forename: S
authors.initials: S
authors.lastname: Rankin
authors.forename: A J
authors.initials: AJ
authors.lastname: Morales
authors.forename: S
authors.initials: S
authors.lastname: Parthasarathy
mesh_terms: D000328:Adult; D004715:Endometriosis / Q000378:metabolism* / Q000473:pathology; D004717:Endometrium / Q000378:metabolism* / Q000473:pathology; D000939:Epitopes / Q000276:immunology; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D008077:Lipoproteins, LDL / Q000276:immunology / Q000378:metabolism*; D012016:Reference Values; D014018:Tissue Distribution
chemical_list: D000939:Epitopes; D008077:Lipoproteins, LDL; C099252:oxidized low density lipoprotein