[Telomere length and telomerase activity in hepatocellular carcinoma].
Telomerase activity and terminal restriction fragment (TRF) length were examined in hepatocellular carcinoma (HCC). Telomerase activity was assayed by telomeric repeat amplification protocol (TRAP) connected with an internal telomerase assay standard (ITAS). The incidence of strong telomerase activity (highly variable level compared with the activity of non-cancerous liver tissue) was 79% in well, 84% in moderately, and 100% in poorly differentiated HCC, while 0% in non-cancerous liver tissues. The incidence of TRF length alteration (reduction or elongation) was 53% in HCC. The incidence of TRF alteration was significantly higher in HCC exceeding 3 cm in diameter, moderately or poorly differentiated in histology. Telomerase activity was not associated with TRF length alteration in HCC. In conclusion, strong telomerase activity and TRF length alteration increased with HCC tumor progressions.
Nakashio
R
R
First Department of Internal Medicine, Hiroshima University School of Medicine.
Kitamoto
M
M
Nakanishi
T
T
Takaishi
H
H
Takahashi
S
S
Kajiyama
G
G
D006528:Carcinoma, Hepatocellular / Q000235:genetics* / Q000473:pathology; D002454:Cell Differentiation; D006801:Humans; D008113:Liver Neoplasms / Q000235:genetics* / Q000473:pathology; D019098:Telomerase / Q000032:analysis*; D016615:Telomere / Q000235:genetics*
D019098:Telomerase