Determination of ciprofloxacin in plasma and urine by HPLC with ultraviolet detection.
A simple, sensitive isocratic method for the detection and quantification of ciprofloxacin in plasma and urine has been developed. The assay consisted of reversed-phase HPLC with ultraviolet detection. Plasma proteins were removed by a fast and efficient procedure. For the urine samples, the only required sample preparation was dilution. Separation was achieved on a C18 reversed-phase column. The quantification limit was 0.01 mg/L in plasma and 0.5 mg/L in urine. This method was sufficiently sensitive for pharmacokinetic studies.
Kamberi
M
M
Department of Clinical Pharmacology and Therapeutics, Oita Medical University, Japan.
Tsutsumi
K
K
Kotegawa
T
T
Nakamura
K
K
Nakano
S
S
D000890:Anti-Infective Agents / Q000097:blood* / Q000493:pharmacokinetics / Q000652:urine*; D002851:Chromatography, High Pressure Liquid; D002939:Ciprofloxacin / Q000097:blood* / Q000493:pharmacokinetics / Q000652:urine*; D006801:Humans; D012680:Sensitivity and Specificity; D013056:Spectrophotometry, Ultraviolet
D000890:Anti-Infective Agents; D002939:Ciprofloxacin