Gscl, a gene within the minimal DiGeorge critical region, is expressed in primordial germ cells and the developing pons.
Gscl, a paired-type homeobox gene, has been implicated in the pathology of DGS/VCFS by virtue of its genomic location and its structural similarity to the Gsc gene family. Immunohistochemical and in situ studies were performed to examine the expression pattern of this gene during embryonic development. A polyclonal antibody, generated to the full-length protein and shown to be specific for GSCL by both Western blotting and immunofluorescence, was used for immunohistochemical localization. Both in situ and antibody staining localized GSCL expression to a cluster of cells in the pons region of the developing brain. This GSCL expression pattern showed partial overlap with that of Pax6. More detailed immunohistochemistry revealed the GSCL in primordial germ cells during migration from the epithelium of the hindgut and later as they colonize the developing gonads. GSCL was not detected in tissues affected in DGS/VCSF.
Galili
N
N
The Wistar Institute, Philadelphia, Pennsylvania 19104, USA. galili@wista.wistar.upenn.edu
Epstein
J A
JA
Leconte
I
I
Nayak
S
S
Buck
C A
CA
D016475:3T3 Cells; D000818:Animals; D000906:Antibodies / Q000276:immunology; D000917:Antibody Formation; D019556:COS Cells; D004268:DNA-Binding Proteins / Q000235:genetics*; D004062:DiGeorge Syndrome / Q000235:genetics*; D005314:Embryonic and Fetal Development / Q000502:physiology; D019084:Fluorescent Antibody Technique, Indirect; D018507:Gene Expression Regulation, Developmental; D005854:Germ Cells; D051956:Goosecoid Protein; D018398:Homeodomain Proteins; D017403:In Situ Hybridization; D051379:Mice; D011149:Pons / Q000196:embryology*; D012097:Repressor Proteins; D014157:Transcription Factors
D000906:Antibodies; D004268:DNA-Binding Proteins; D051956:Goosecoid Protein; C497993:Gsc protein, mouse; D018398:Homeodomain Proteins; D012097:Repressor Proteins; D014157:Transcription Factors