Structure and expression of the human septin gene HCDCREL-1.
Septins are a family of highly conserved filament-forming proteins that have been shown to mediate cytokinesis and cytoskeletal organization in fungi and Drosophila. The gene encoding the human septin family member HCDCREL-1 has been shown to be transcribed from a locus immediately adjacent to that of the platelet glycoprotein (GP) Ib b. The HCDCREL-1 gene possesses a non-consensus polyadenylation signal that apparently is not efficiently utilized, resulting in the expression of a readthrough transcript also containing the platelet GPIb beta coding region. As a first step in understanding the regulation and function of HCDCREL-1, we have analyzed the structure of this gene and characterized its expression in a variety of human cells. Our results indicate that the gene is expressed at high levels in platelets and neural tissue, and is transcriptionally complex.
Yagi
M
M
Research, Department of Veterans Affairs Puget Sound Health Care Center, Seattle, WA 98108, USA. myagi@u.washington.edu
Zieger
B
B
Roth
G J
GJ
Ware
J
J
D001483:Base Sequence; D001792:Blood Platelets / Q000378:metabolism; D001921:Brain / Q000378:metabolism; D018797:Cell Cycle Proteins / Q000096:biosynthesis* / Q000737:chemistry / Q000235:genetics*; D003001:Cloning, Molecular; D017931:DNA Primers; D018076:DNA, Complementary / Q000737:chemistry / Q000302:isolation & purification; D015870:Gene Expression; D006801:Humans; D004915:Leukemia, Erythroblastic, Acute; D008099:Liver / Q000378:metabolism; D008969:Molecular Sequence Data; D009928:Organ Specificity / Q000235:genetics; D010920:Placenta / Q000378:metabolism; D012260:Ribonucleases; D058112:Septins; D014407:Tumor Cells, Cultured
D018797:Cell Cycle Proteins; D017931:DNA Primers; D018076:DNA, Complementary; D012260:Ribonucleases; C109366:SEPTIN5 protein, human; D058112:Septins