In vitro screening model for the detection of agents active against myelogenous leukemia.
This study was directed at defining the optimal in vitro screening methodology for the selection of new anti-myelogenous leukemia agents. Using thousands of samples of synthetic compounds from the Eastman Pharmaceutical, Inc. and Sterling Research Corporation (Eastman/Sterling) inventory, a number of sequential combinations of 8 cell lines were employed to optimize the model. The cells in these lines were of either murine (L1210 lymphocytic leukemia, C1498 myelogenous leukemia, and colony-forming unit-granulocyte macrophage [CFU-GM]) or human (HL-60, K-562, HEL, and THP-1 myelogenous leukemias, and CFU-GM) origin. The focus of the study was to find the most efficient and cost-effective manner in which to test compounds for both cytotoxicity against the tumor cells and for differential cytotoxicity against tumor as compared with normal cells.
Edelstein
M
M
Department of Medicine, Wayne State University, Detroit, Michigan, USA.
Corbett
T
T
Valeriote
F
F
D000818:Animals; D004354:Drug Screening Assays, Antitumor / Q000379:methods*; D006801:Humans; D007951:Leukemia, Myeloid / Q000188:drug therapy*; D051379:Mice; D014407:Tumor Cells, Cultured