title: Diagnosis of sickle-cell disease by DNA analysis in Nigeria.
abstract: OBJECTIVE
  To compare genotyping of leucocyte DNA by PCR and Dde I with the conventional genotyping of haemoglobin of the same subject.
  
  
  METHODS
  Comparative study.
  
  
  METHODS
  25 adults, 16 males and nine females.
  
  
  METHODS
  Predictability.
  
  
  RESULTS
  In all cases the results were comparable.
  
  
  CONCLUSIONS
  The technology can be applied locally to prenatal diagnosis of sickle-cell disease by chorionic sampling and, therefore, improve the management of sickle-cell disease in Nigeria.
authors.affiliation: Nigerian Institute of Medical Research, Yaba-Lagos, Nigeria.
authors.forename: T A
authors.initials: TA
authors.lastname: Adewole
authors.forename: D K
authors.initials: DK
authors.lastname: Olukoya
authors.forename: A
authors.initials: A
authors.lastname: Afonja
mesh_terms: D000328:Adult; D000755:Anemia, Sickle Cell / Q000175:diagnosis* / Q000235:genetics*; D016022:Case-Control Studies; D015193:Chorionic Villi Sampling; D004247:DNA / Q000032:analysis*; D004588:Electrophoresis, Cellulose Acetate; D005260:Female; D005838:Genotype; D006801:Humans; D007962:Leukocytes; D008297:Male; D009549:Nigeria; D016133:Polymerase Chain Reaction / Q000379:methods*; D015203:Reproducibility of Results; D015183:Restriction Mapping / Q000379:methods*
chemical_list: D004247:DNA