N(G)-nitro-L-arginine methyl ester reduces stress-related feeding in the rat tail-pinch model.
A possible role of nitric oxide (NO) in stress-related feeding was investigated in male rats using the tail-pinch (TP) model, in within-subjects experimental designs. An initial experiment demonstrated a dose-related reduction in TP-induced solid food intake over a 10-min test period with increasing dose (10.25, and 50 mg/kg SC) of the NO-synthase (NOS) inhibitor, N(G)-nitro-L-arginine methyl ester (L-NAME), reaching statistical significance at 25 mg/kg L-NAME when compared to vehicle control (p < 0.05). Pattern analysis further revealed a decrease both in total duration of food-directed oral behavior and in percentage of longer duration (> 60 s) oral behavior bouts with increasing dose of L-NAME; both measures reached statistical significance at 50 mg/kg (p < 0.01). Pretreatment with 500 mg/kg of the NO precursor, L-arginine (L-arg), resulted in partial but not significant reversal of the attenuating effect of 25 mg/kg L-NAME on food intake. Latency to begin eating or gnawing was not significantly affected by L-NAME. In a subsequent experiment, L-arg alone (500 and 750 mg/kg) did not significantly alter TP-induced food intake. It is cautiously suggested that these results implicate involvement of NO in TP-induced feeding.
Czech
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Department of Psychology, Marquette University, Milwaukee, WI 53201-1881, USA.
Klosterman
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Le Sueur
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D000818:Animals; D004195:Disease Models, Animal; D004435:Eating / Q000187:drug effects / Q000502:physiology; D004791:Enzyme Inhibitors / Q000494:pharmacology*; D005247:Feeding Behavior / Q000187:drug effects* / Q000502:physiology; D008297:Male; D019331:NG-Nitroarginine Methyl Ester / Q000494:pharmacology*; D009569:Nitric Oxide / Q000502:physiology; D019001:Nitric Oxide Synthase / Q000037:antagonists & inhibitors*; D051381:Rats; D017207:Rats, Sprague-Dawley; D013315:Stress, Psychological / Q000188:drug therapy* / Q000503:physiopathology
D004791:Enzyme Inhibitors; D009569:Nitric Oxide; D019001:Nitric Oxide Synthase; D019331:NG-Nitroarginine Methyl Ester