Two-dimensional polyacrylamide gel electrophoresis map of bull seminal plasma proteins.
A two-dimensional polyacrylamide gel electrophoresis map of bull seminal plasma proteins has been established. About 250 spots were detected after silver staining and polypeptides from 24 spots have been N-terminally sequenced. Major proteins already described in bull seminal plasma, like PDC-109 and aSFP, have been located on the map; proteins not yet reported in male reproductive tracts have been evidenced; for some polypeptides showing a previously unknown N-terminal sequence, structural similarities with proteins described in other organisms have been found. A reference map of seminal plasma proteins could be useful in relating protein pattern changes to physiopathological events influencing the reproductive sphere.
Mortarino
M
M
Istituto di Fisiologia Veterinaria e Biochimica, Università degli Studi di Milano, Italy.
Tedeschi
G
G
Negri
A
A
Ceciliani
F
F
Gottardi
L
L
Maffeo
G
G
Ronchi
S
S
D000595:Amino Acid Sequence; D000818:Animals; D002417:Cattle; D015180:Electrophoresis, Gel, Two-Dimensional / Q000379:methods*; D008297:Male; D008969:Molecular Sequence Data; D010449:Peptide Mapping / Q000379:methods*; D029584:Prostatic Secretory Proteins; D011506:Proteins / Q000032:analysis*; D029589:Seminal Plasma Proteins
D029584:Prostatic Secretory Proteins; D011506:Proteins; D029589:Seminal Plasma Proteins; C043076:beta-microseminoprotein