Influence of charge variation in the Streptomyces venezuelae alpha-amylase signal peptide on heterologous protein production by Streptomyces lividans.
With the aim of investigating determining factors for secretion of heterologous proteins by streptomycetes, we analysed the effect of charge variation in the Streptomyces venezuelae ATCC15068 alpha-amylase signal peptide on expression and secretion of mouse tumour necrosis factor alpha (mTNF) by Streptomyces lividans. To this end, the mTNF cDNA was fused to the wild-type alpha-amylase (aml) signal sequence and the fusion gene was expressed under the control of the S. venezuelae CBS762.70 subtilisin inhibitor gene (vsi) promoter, which has been shown to be very effective in initiating transcription. In addition, the number of positive charges in the N region of the alpha-amylase signal peptide was altered by in vitro mutagenesis. Secreted and intracellular mTNF levels were determined by sodium dodecyl sulphate/polyacrylamide gel electrophoresis and biological activity measurements. This revealed moderate amounts of secreted mTNF compared to the levels obtained in previous experiments using the vsi promoter in combination with the Vsi signal peptide. Levels of secreted mTNF could be increased sevenfold by introducing one extra positive charge in the N region of the signal peptide.
Lammertyn
E
E
Laboratory of Bacteriology, Rega Instituut, K.U.Leuven, Belgium.
Desmyter
S
S
Schacht
S
S
Van Mellaert
L
L
Anné
J
J
D000818:Animals; D005822:Genetic Vectors; D015151:Immunoblotting; D051379:Mice; D016254:Mutagenesis, Insertional / Q000235:genetics; D021382:Protein Sorting Signals / Q000235:genetics; D011994:Recombinant Proteins / Q000378:metabolism*; D013302:Streptomyces / Q000201:enzymology / Q000235:genetics* / Q000254:growth & development; D014158:Transcription, Genetic; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism*; D000516:alpha-Amylases / Q000737:chemistry* / Q000235:genetics
D021382:Protein Sorting Signals; D011994:Recombinant Proteins; D014409:Tumor Necrosis Factor-alpha; D000516:alpha-Amylases