Direct identification of each specific mutation in codon 12 and 13 of ci-ki-ras2 by SSCP analysis.
We compared the SSCP behaviour of the DNA fragments containing c-ki-ras 2 wild type 12 and 13 codons or each of the 12 possible point mutated sequences in these two codons. We found that a single electrophoresis condition was sufficient to distinguish each specific mutation from the other 11 and from the wild type sequence. This observation makes it possible to identify each specific mutation directly by SSCP without any need for reamplification and sequencing.
La Farina
M
M
Dipartimento di Biologia Cellulare e dello Sviluppo, Università di Palermo viale delle Scienze II, Italy. mariolaf@mbox.unipa.it
Maturi
N
N
Stira
S
S
Russo
A
A
Bazan
V
V
Albanese
I
I
D002277:Carcinoma / Q000235:genetics; D003001:Cloning, Molecular; D003062:Codon; D015179:Colorectal Neoplasms / Q000235:genetics*; D004273:DNA, Neoplasm / Q000302:isolation & purification; D004591:Electrophoresis, Polyacrylamide Gel; D005787:Gene Frequency; D011905:Genes, ras; D006801:Humans; D009154:Mutation; D018807:Polymorphism, Single-Stranded Conformational; D016283:Proto-Oncogene Proteins p21(ras) / Q000235:genetics*
D003062:Codon; D004273:DNA, Neoplasm; C501009:HRAS protein, human; D016283:Proto-Oncogene Proteins p21(ras)