[Detection of Borrelia DNA in urine using polymerase chain reaction in rheumatologic laboratory diagnosis of Lyme borreliosis].
Borrelia burgdorferi specific DNA has been detected by polymerase chain reaction (PCR) in different specimens of patients with Lyme disease (LD). The aim of the present study is to evaluate PCR-diagnostic of urine specimens regarding rheumatologic diagnosis of Lyme disease. Urine specimens of 77 patients (LD, n = 34; undifferentiated arthritis (UA), n = 25; arthralgia/myalgia (AM), n = 18) and 15 controls were investigated. Flagellin gene (60 specimens) or OspA-plasmid (32 specimens) were used as targets. Sensitivity of the flagellin-nested-PCR was 27%, by OspA-nested-PCR only one positive PCR result was found. Despite of low sensitivity PCR enabled the correct diagnosis of LD in two patients classified as UA. Therefore, PCR can give valuable hints in single cases if LD is clinically suspected.
Schnarr
S
S
Abteilung Rheumatologie, Medizinische Hochschule Hannover, Zentrum Innere Médizin und Dermatologie.
Hopf
S
S
Zeidler
H
H
Hammer
M
M
D000328:Adult; D018771:Arthralgia / Q000175:diagnosis / Q000652:urine; D001168:Arthritis / Q000175:diagnosis / Q000652:urine; D015748:Borrelia burgdorferi Group / Q000235:genetics*; D004269:DNA, Bacterial / Q000652:urine*; D003937:Diagnosis, Differential; D005260:Female; D006801:Humans; D008193:Lyme Disease / Q000175:diagnosis* / Q000652:urine; D008297:Male; D016133:Polymerase Chain Reaction; D012680:Sensitivity and Specificity
D004269:DNA, Bacterial
Nachweis von Borrelien-DNA im Urin mittels Polymerase-Kettenreaktion in der rheumatologischen Labordiagnostik der Lyme-Borreliose.