[Association of phosphoinositide-specific phospholipase Cgamma1 with elements of cytoskeleton in A-431 cells].
It is shown that phosphoinositide-specific phospholipase C gamma 1 (PLC gamma 1), a substrate of growth factor receptors, is associated with cytoskeleton in A-431 cells. PLC gamma 1 is co-localized only with the cortical actin but not with actin stress fibers. Since EGF receptor is also co-localized with the cortical actin it is concluded that PLC gamma 1 co-localized with actin is mediated by the EGF receptor. After the treatment with cytochalasin B PLC gamma 1 is co-localized with actin aggregates and cytoskeleton elements other than actin. Using double immunofluorescence PLC gamma 1 is shown to be associated with cytokeratin intermediate filaments. The cross-talking of different cytoskeleton elements and their participation in cell signaling is discussed.
Chupreta
S V
SV
Institute of Cytology, Russian Academy of Sciences, St. Petersburg.
Evdonin
A L
AL
Tvorogov
D V
DV
Tsupkina
N V
NV
Nikol'nskiĭ
N N
NN
Medvedeva
N D
ND
D000199:Actins / Q000032:analysis; D002294:Carcinoma, Squamous Cell / Q000737:chemistry / Q000473:pathology; D003599:Cytoskeleton / Q000737:chemistry*; D003902:Detergents; D066246:ErbB Receptors / Q000032:analysis; D005455:Fluorescent Antibody Technique; D007527:Isoenzymes / Q000737:chemistry*; D043265:Phosphatidylinositol Diacylglycerol-Lyase; D012995:Solubility; D014407:Tumor Cells, Cultured; D010738:Type C Phospholipases / Q000737:chemistry*
D000199:Actins; D003902:Detergents; D007527:Isoenzymes; D066246:ErbB Receptors; D010738:Type C Phospholipases; D043265:Phosphatidylinositol Diacylglycerol-Lyase
Sviaz' fosfoinozitid-spetsificheskoĭ fosfolipazy Cgamma1 s élementami tsitoekelta v kletkah A-431.