Transduction of normal and malignant oral epithelium by particle bombardment.
Although genetic approaches to the treatment and prevention of oral cancer are being developed, there are no suitable methods of transduction of the oral mucosa or early cancers. We therefore tested the technique of particle bombardment for its ability to transduce oral cancer cells in vitro and normal epithelium of the hamster cheek pouch in vivo. A gene gun was used to transfer a plasmid that encoded a marker/suicide fusion gene, beta-galactosidase-thymidine kinase (GAL-TEK), under control of a CMV promoter. For comparison we used the method of lipofection and an adenovirus vector. Particle bombardment transduced up to 13% of cells in culture, resulting in a 24.3% reduction in growth in the presence of ganciclovir. The efficiency of transduction was similar to that of lipofection but was much less than that of the adenovirus vector, which transduced 54% of cells and completely inhibited their growth in the presence of ganciclovir. Transduction of the hamster cheek pouch by particle bombardment produced expression of beta-galactosidase as judged by macroscopic staining, for up to 5 days. However, histological examination showed that the transduced cells were rare and superficial, and that administration of systemic ganciclovir did not lead to any changes in the tissue. Improvements in efficiency are necessary before the gene gun can be used in the management of oral cancer.
Shillitoe
E J
EJ
Department of Microbiology and Immunology, State University of New York College of Medicine, Syracuse 13210, USA.
Noonan
S
S
Hinkle
C C
CC
Marini
F C
FC
Kellman
R M
RM
D000818:Animals; D002522:Chlorocebus aethiops; D006224:Cricetinae; D015774:Ganciclovir / Q000494:pharmacology; D006801:Humans; D008647:Mesocricetus; D009061:Mouth Mucosa / Q000528:radiation effects*; D009062:Mouth Neoplasms / Q000473:pathology*; D014161:Transduction, Genetic / Q000528:radiation effects*; D014407:Tumor Cells, Cultured; D014709:Vero Cells; D001616:beta-Galactosidase / Q000235:genetics
D001616:beta-Galactosidase; D015774:Ganciclovir