Analysis of lysergic acid diethylamide: comparison of capillary electrophoresis with laser-induced fluorescence (CE-LIF) with conventional techniques.
The increasing use of the potent hallucinogenic drug D-lysergic acid diethylamide (LSD) makes the application of highly sensitive analytical techniques necessary. For an effective analysis of biological specimens a limit of detection in the sub-ng/ml range is required. Using capillary electrophoresis (CE), with laser-induced fluorescence detection it was possible to determine 0.1-0.2 ng LSD/ml blood. The conventionally used immunoassays and gas and high-performance liquid chromatographic methods have cut-off values and limit of detection, respectively, in the same range. Only tandem mass spectrometry techniques can be more sensitive. Therefore, CE can be considered a good complementary method to conventional immunological and chromatographic techniques for the forensic analysis of biofluids.
Frost
M
M
Westfälische Wilhelms-Universität Münster, Institut für Rechtsmedizin, Germany. frost@uni-muenster.de
Köhler
H
H
D001774:Blood Chemical Analysis / Q000379:methods; D019075:Electrophoresis, Capillary / Q000379:methods*; D005453:Fluorescence; D005554:Forensic Medicine / Q000379:methods*; D006213:Hallucinogens / Q000032:analysis*; D006801:Humans; D007834:Lasers; D008238:Lysergic Acid Diethylamide / Q000032:analysis*; D012680:Sensitivity and Specificity
D006213:Hallucinogens; D008238:Lysergic Acid Diethylamide