Effect of microtubule disruption on cell adhesion and spreading.
Microtubules have been involved in a variety of cellular processes. In this study, we examined the role of the microtubular system in the adhesion and spreading of the adenocarcinoma cell line HT29-D4. Disruption of microtubules by nocodazole or navelbine resulted in an increase in cell adhesion to purified ECM proteins. This enhanced cell adhesion is mediated by integrins, but is not attributable to quantitative changes in the number of integrin receptors at the cell surface, as determined by flow cytometric analysis. In contrast to attachment, spreading of HT29-D4 cells was reduced by nocodazole treatment in a dose-dependent manner. Thus, microtubule depolymerization appears to increase initial attachment of cells to extracellular matrix, while impeding subsequent cell spreading.
Kadi
A
A
UPRES A-CNRS 6032, Faculté de Pharmacie, Marseille, France.
Pichard
V
V
Lehmann
M
M
Briand
C
C
Braguer
D
D
Marvaldi
J
J
Rognoni
J B
JB
Luis
J
J
D000230:Adenocarcinoma / Q000378:metabolism*; D000970:Antineoplastic Agents / Q000494:pharmacology; D002448:Cell Adhesion; D048429:Cell Size; D000077143:Docetaxel; D004305:Dose-Response Relationship, Drug; D006801:Humans; D016023:Integrins / Q000378:metabolism*; D008870:Microtubules / Q000187:drug effects / Q000378:metabolism*; D009362:Neoplasm Metastasis; D015739:Nocodazole / Q000494:pharmacology; D017239:Paclitaxel / Q000031:analogs & derivatives / Q000494:pharmacology; D043823:Taxoids; D014407:Tumor Cells, Cultured; D014747:Vinblastine / Q000031:analogs & derivatives / Q000494:pharmacology; D000077235:Vinorelbine
D000970:Antineoplastic Agents; D016023:Integrins; D043823:Taxoids; D000077143:Docetaxel; D014747:Vinblastine; D017239:Paclitaxel; D000077235:Vinorelbine; D015739:Nocodazole