The effect of protein kinase C inhibitors on invasion of human ovary cancer cells.
In the present work we tested whether invasiveness of ovarian carcinoma cells could be considered as protein kinase C (PKC) dependent process. The migration and invasion studies were performed in Transwell chambers. Staurosporine, sphingosine and tamoxifen were used as PKC inhibitors. Also the effect of prolonged treatment with TPA was the subject of observation. The obtained results indicated that invasion understood as three step process (attachment, migration and matrix degradation) was affected by PKC inhibitors. The detailed studies, however, showed that attachment and matrix degradation ability of ovarian cancer cells was not changed by PKC inhibitors as opposed to migration which was, at least partly, regulated by protein kinase C.
Szaniawska
B
B
Department of Cell Biology, The Maria Sklodowska-Curie Cancer Center, Warsaw, Poland.
Gawrychowski
K
K
Janik
P
P
D000368:Aged; D002448:Cell Adhesion / Q000187:drug effects; D002465:Cell Movement / Q000187:drug effects; D003094:Collagen; D015536:Down-Regulation; D004791:Enzyme Inhibitors / Q000494:pharmacology*; D005260:Female; D005353:Fibronectins; D006801:Humans; D009361:Neoplasm Invasiveness; D009362:Neoplasm Metastasis; D010051:Ovarian Neoplasms / Q000188:drug therapy* / Q000473:pathology; D011493:Protein Kinase C / Q000037:antagonists & inhibitors*; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology; D014407:Tumor Cells, Cultured
D004791:Enzyme Inhibitors; D005353:Fibronectins; D003094:Collagen; D011493:Protein Kinase C; D013755:Tetradecanoylphorbol Acetate