Association of genes encoding P fimbriae, CS31A antigen and EAST 1 toxin among CNF1-producing Escherichia coli strains from cattle with septicemia and diarrhea.
Fifty-six CNF1-producing Escherichia coli strains isolated from cattle with diarrhea or septicemia were screened by PCR for the detection of pap, sfa, afa, clpG, or f17 adherence factor and EAST 1 toxin genes. All the isolates were pap-positive, in accordance with the close association of pap, CNF1 and alpha-hemolysin genes observed on human and porcine E. coli. Only the gene encoding the P adhesin of class III (PrsG) was detected. Genes encoding CS31A antigen (71%) and S fimbriae (34%) (but not Afa or F17) were detected among the bovine isolates. E. coli producing both CNF1 and plasmid-encoded CS31A is a new example of association between bacterial clones and plasmid-mediated virulence factors. The EAST 1 toxin-encoding gene was detected on 66% of the CNF1-producing isolates but was linked to CS31A rather than to CNF1. These results suggest a close association between EAST 1 toxin and the adherence factor CS31A among pathogenic bovine E. coli.
Bertin
Y
Y
Laboratoire de Microbiologie, Centre de Recherche de Clermont-Ferrand-Theix, Saint-Genès-Champanelle, France. bertin@clermont.inra.fr
Martin
C
C
Girardeau
J P
JP
Pohl
P
P
Contrepois
M
M
D018830:Adhesins, Escherichia coli / Q000235:genetics*; D000818:Animals; D000942:Antigens, Bacterial; D016470:Bacteremia / Q000382:microbiology / Q000662:veterinary; D001426:Bacterial Proteins / Q000235:genetics; D001427:Bacterial Toxins / Q000235:genetics* / Q000378:metabolism; D001483:Base Sequence; D002417:Cattle; D002418:Cattle Diseases / Q000382:microbiology; D003603:Cytotoxins / Q000378:metabolism; D003967:Diarrhea / Q000382:microbiology / Q000662:veterinary; D004926:Escherichia coli / Q000235:genetics*; D029968:Escherichia coli Proteins; D005798:Genes, Bacterial / Q000235:genetics*; D008969:Molecular Sequence Data; D011971:Receptors, Immunologic / Q000235:genetics*; D016415:Sequence Alignment
D018830:Adhesins, Escherichia coli; D000942:Antigens, Bacterial; D001426:Bacterial Proteins; D001427:Bacterial Toxins; D003603:Cytotoxins; D029968:Escherichia coli Proteins; D011971:Receptors, Immunologic; C037872:pili, bacterial receptor; C060296:cytotoxic necrotizing factor type 1; C071730:clpG protein, E coli