Signal transduction by the peptide which mimics the activity of thrombopoietin.
Thrombopoietin (TPO) plays a central role in megakaryopoiesis and platelet production. It is a ligand for c-mpl, which is a member of the hematopoietic receptor superfamily. We have recently identified several human c-mpl binding peptides which are distinct from TPO, from phage random peptide libraries. PK1M is one of these peptides and is an agonist of c-mpl which is TPO receptor. We show here that PK1M induces the tyrosine phosphorylation of the Janus kinase 2 (JAK2) and the activation of the signal transducer and activation of transcription 5 (STAT5) in TPO-dependent cells like TPO.
Kimura
T
T
Research and Development Division, Hokuriku Seiyaku Co., Ltd., Katsuyama, Fukui, Japan.
Kaburaki
H
H
Tsujino
T
T
Watanabe
Y
Y
Kato
H
H
D000595:Amino Acid Sequence; D000818:Animals; D001665:Binding Sites; D004268:DNA-Binding Proteins / Q000378:metabolism; D004789:Enzyme Activation; D006801:Humans; D036341:Intercellular Signaling Peptides and Proteins; D053614:Janus Kinase 2; D051379:Mice; D008894:Milk Proteins; D009363:Neoplasm Proteins; D010455:Peptides / Q000494:pharmacology* / Q000502:physiology; D010766:Phosphorylation; D011505:Protein-Tyrosine Kinases / Q000378:metabolism; D011518:Proto-Oncogene Proteins / Q000819:agonists; D018121:Receptors, Cytokine; D053628:Receptors, Thrombopoietin; D050799:STAT5 Transcription Factor; D015398:Signal Transduction / Q000502:physiology*; D013268:Stimulation, Chemical; D013926:Thrombopoietin / Q000502:physiology*; D015534:Trans-Activators / Q000378:metabolism
D004268:DNA-Binding Proteins; D036341:Intercellular Signaling Peptides and Proteins; D008894:Milk Proteins; D009363:Neoplasm Proteins; C113563:PK1M peptide; D010455:Peptides; D011518:Proto-Oncogene Proteins; D018121:Receptors, Cytokine; D053628:Receptors, Thrombopoietin; D050799:STAT5 Transcription Factor; D015534:Trans-Activators; C082218:MPL protein, human; D013926:Thrombopoietin; D011505:Protein-Tyrosine Kinases; C507924:JAK2 protein, human; C507926:Jak2 protein, mouse; D053614:Janus Kinase 2