title: Simple, rapid and sensitive determination of protionamide in human serum by high-performance liquid chromatography.
abstract: A simple high-performance liquid chromatography method has been developed that allows the sensitive determination of protionamide (2-n-propyl-pyridine-4-carboxylic acid thioamide, PTH) in human serum. After pretreatment of the serum with trichloroacetic acid (TCA) and centrifugation the supernatants were neutralized using NaHCO3. PTH was separated on a Kromasil 100 C4 column (acetonitrile-sodium tetraborate buffer pH 8-dibutylamine) and determined photometrically at 291 nm. The lower limit of quantification for 300 microl serum precipitated with 60 microl TCA and injection of 50 microl was 27 microg/l and linearity was observed up to 15 mg/l.
authors.affiliation: Borstel Research Center, Germany.
authors.forename: H
authors.initials: H
authors.lastname: Bartels
authors.forename: R
authors.initials: R
authors.lastname: Bartels
mesh_terms: D000995:Antitubercular Agents / Q000097:blood*; D002851:Chromatography, High Pressure Liquid; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007202:Indicators and Reagents; D011515:Prothionamide / Q000097:blood*; D015203:Reproducibility of Results; D013056:Spectrophotometry, Ultraviolet; D014238:Trichloroacetic Acid
chemical_list: D000995:Antitubercular Agents; D007202:Indicators and Reagents; D014238:Trichloroacetic Acid; D011515:Prothionamide