Amyloid beta-peptide(25-35) changes [Ca2+] in hippocampal neurons.
Insoluble aggregates of the amyloid beta-peptide (A beta) is a major constituent of senile plaques found in brains of Alzheimer disease (AD) patients. The detrimental effects of aggregated A beta is associated with an increased intracellular Ca2+ concentration ([Ca2+]i). We examined the effects of A beta(25-35) on [Ca2+]i and intracellular H+ concentration ([H+]i) in single hippocampal neurons by real time fluorescence imaging using the Ca(2+)- and H(+)-specific ratio dyes, indo-1 and SNARF-1. Incubation of these cultures with A beta(25-35) for 3-12 days in vitro increased [Ca2+]i and [H+]i in large, NMDA-responsive neurons.
Mogensen
H S
HS
Department of Pharmacology, University of Copenhagen, Denmark.
Beatty
D M
DM
Morris
S J
SJ
Jorgensen
O S
OS
D016229:Amyloid beta-Peptides / Q000494:pharmacology*; D000818:Animals; D000831:Animals, Newborn; D001253:Astrocytes / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D001578:Benzopyrans; D002118:Calcium / Q000378:metabolism*; D002470:Cell Survival; D002478:Cells, Cultured; D005456:Fluorescent Dyes; D005904:Glial Fibrillary Acidic Protein / Q000032:analysis; D006624:Hippocampus / Q000166:cytology / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D007211:Indoles; D007700:Kinetics; D008856:Microscopy, Fluorescence; D009284:Naphthols; D009474:Neurons / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D010446:Peptide Fragments / Q000494:pharmacology*; D051381:Rats; D012235:Rhodamines; D013997:Time Factors
D016229:Amyloid beta-Peptides; D001578:Benzopyrans; D005456:Fluorescent Dyes; D005904:Glial Fibrillary Acidic Protein; D007211:Indoles; D009284:Naphthols; D010446:Peptide Fragments; D012235:Rhodamines; C074636:amyloid beta-protein (25-35); C091666:seminaphthorhodaminefluoride; C048960:indo-1; D002118:Calcium