title: Protein profiles of Malassezia pachydermatis isolated from dogs.
abstract: The protein fractions of 30 Malassezia pachydermatis isolates from dogs (15 with otitis and 15 with dermatitis) were investigated by sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE). The electrophoresis runs resulted in bands ranging from 15.0-145.7 kDa in the ear canal secretion isolates. The values for the skin isolates varied between 16.0-141.1 kDa. The presence of protein bands occurred most frequently within the 30-40 kDa bands (66.7%), 40-50 kDa (70.0%), 50-60 kDa (73.3%) and 80-90 kDa (83.3%). No statistical differences were determined, considering the behavior of M. pachydermatis isolates from ear and skin in each band of molecular weight values, nor in the number of bands obtained.
authors.affiliation: Microbiology Department, Biomedical Science Institute, Universidade de São Paulo, Brazil.
authors.forename: S D
authors.initials: SD
authors.lastname: Coutinho
authors.forename: T
authors.initials: T
authors.lastname: de Souza
authors.forename: C R
authors.initials: CR
authors.lastname: Paula
mesh_terms: D000818:Animals; D004285:Dogs; D004424:Ear Canal / Q000382:microbiology; D004591:Electrophoresis, Polyacrylamide Gel; D005656:Fungal Proteins / Q000032:analysis*; D008290:Malassezia / Q000737:chemistry*; D008565:Membrane Proteins / Q000032:analysis*; D012867:Skin / Q000382:microbiology; D018709:Statistics, Nonparametric
chemical_list: D005656:Fungal Proteins; D008565:Membrane Proteins