title: A probarley lectin processing enzyme purified from Arabidopsis thaliana seeds.
abstract: An aspartic proteinase was purified from the seeds of Arabidopsis thaliana (ecotype RLD) using affinity chromatography on pepstatin-agarose and ion exchange chromatography. The purified enzyme is optimally active at pH 3.5 and completely inhibited by pepstatin A. The purified Arabidopsis aspartic proteinase contains four subunits (apparent molecular weights 31, 28.5, 15 and 6 kDa), two of which are probably linked by disulfide bridges. These properties are similar to the aspartic proteinase previously isolated from barley seeds. The amino acid sequence of the peptide subunits corresponds exactly with the sequence of the previously isolated cDNA for the Arabidopsis aspartic proteinase. The Arabidopsis enzyme processed probarley lectin in vitro at the carboxy-terminus between phenylalanine and alanine, the same place where the barley enzyme cleaves the lectin in vitro. The aspartic proteinase appears to be the major enzyme processing the lectin in seeds as pepstatin A inhibited this activity in a crude seed extract.
authors.affiliation: Department of Biological Sciences, State University of New York at Binghamton 13902-6000, USA.
authors.forename: A
authors.initials: A
authors.lastname: Mutlu
authors.forename: J E
authors.initials: JE
authors.lastname: Pfeil
authors.forename: S
authors.initials: S
authors.lastname: Gal
mesh_terms: D000595:Amino Acid Sequence; D017360:Arabidopsis / Q000201:enzymology* / Q000378:metabolism; D016282:Aspartic Acid Endopeptidases / Q000037:antagonists & inhibitors / Q000737:chemistry / Q000302:isolation & purification* / Q000378:metabolism; D002846:Chromatography, Affinity; D018076:DNA, Complementary; D006863:Hydrogen-Ion Concentration; D037102:Lectins / Q000378:metabolism*; D008969:Molecular Sequence Data; D010449:Peptide Mapping; D037121:Plant Lectins; D012639:Seeds / Q000378:metabolism
chemical_list: D018076:DNA, Complementary; D037102:Lectins; D037121:Plant Lectins; C076978:barley lectin; D016282:Aspartic Acid Endopeptidases