title: Regulation of energy transduction and electron transfer in cytochrome c oxidase by adenine nucleotides.
abstract: Cytochrome c oxidase from bovine heart contains seven high-affinity binding sites for ATP or ADP and three additional only for ADP. One binding site for ATP or ADP, located at the matrix-oriented domain of the heart-type subunit VIaH, increases the H+/e- stoichiometry of the enzyme from heart or skeletal muscle from 0.5 to 1.0 when bound ATP is exchanged by ADP. Two further binding sites for ATP or ADP, located at the cytosolic and the matrix domain of subunit IV, increases the K(M) for cytochrome c and inhibit the respiratory activity at high ATP/ADP ratios, respectively. We propose that thermogenesis in mammals is related to subunit VIaL of cytochrome c oxidase with a H+/e- stoichiometry of 0.5 compared to 1.0 in the enzyme from bacteria or ectotherm animals. This hypothesis is supported by the lack of subunit VIa isoforms in cytochrome c oxidase from fish.
authors.affiliation: Fachbereich Chemie, Philipps-Universität, Marburg, Germany.
authors.forename: B
authors.initials: B
authors.lastname: Kadenbach
authors.forename: J
authors.initials: J
authors.lastname: Napiwotzki
authors.forename: V
authors.initials: V
authors.lastname: Frank
authors.forename: S
authors.initials: S
authors.lastname: Arnold
authors.forename: S
authors.initials: S
authors.lastname: Exner
authors.forename: M
authors.initials: M
authors.lastname: Hüttemann
mesh_terms: D000227:Adenine Nucleotides / Q000378:metabolism*; D000818:Animals; D002417:Cattle; D004579:Electron Transport; D003576:Electron Transport Complex IV / Q000378:metabolism*; D005399:Fishes; D009206:Myocardium / Q000201:enzymology*
chemical_list: D000227:Adenine Nucleotides; D003576:Electron Transport Complex IV