title: High-throughput DNA sequencing on a capillary array electrophoresis system.
abstract: A capillary array electrophoresis apparatus capable of running and analyzing 48 DNA sequencing samples simultaneously has been constructed. The instrument uses a replaceable sieving buffer and incorporates a convenient method for introducing the buffer into the capillaries. Data from laser-induced fluorescence are collected as four separate images, one for each optical channel. The integrated data analysis software employs an open architecture that allows use of any DNA base-calling algorithm. DNA sequencing runs are completed in approx. 1 hr (approximately 500 bases), and instrument turnaround time between runs is less than 15 min. Overall, the instrument throughput is on the order of 720 templates/day, or 360,000 bases/day.
authors.affiliation: Molecular Dynamics, Sunnyvale, CA, USA.
authors.forename: M
authors.initials: M
authors.lastname: Marsh
authors.forename: O
authors.initials: O
authors.lastname: Tu
authors.forename: V
authors.initials: V
authors.lastname: Dolnik
authors.forename: D
authors.initials: D
authors.lastname: Roach
authors.forename: N
authors.initials: N
authors.lastname: Solomon
authors.forename: K
authors.initials: K
authors.lastname: Bechtol
authors.forename: P
authors.initials: P
authors.lastname: Smietana
authors.forename: L
authors.initials: L
authors.lastname: Wang
authors.forename: X
authors.initials: X
authors.lastname: Li
authors.forename: P
authors.initials: P
authors.lastname: Cartwright
authors.forename: A
authors.initials: A
authors.lastname: Marks
authors.forename: D
authors.initials: D
authors.lastname: Barker
authors.forename: D
authors.initials: D
authors.lastname: Harris
authors.forename: J
authors.initials: J
authors.lastname: Bashkin
mesh_terms: D000818:Animals; D019075:Electrophoresis, Capillary / Q000295:instrumentation*; D006801:Humans; D017422:Sequence Analysis, DNA / Q000295:instrumentation* / Q000379:methods*