title: Knee joint immobilization decreases aggrecan gene expression in the meniscus.
abstract: Aggrecan is the major proteoglycan of the meniscus, and its primary function is to give the meniscus its viscoelastic compressive properties. The objective of this study was to determine the effect of joint immobilization on aggrecan gene expression in the meniscus. The right hindlimbs of six mature beagles were knee cast-immobilized in 90 degrees of flexion and supported by a sling to prevent weightbearing, while the contralateral limb was left free to bear weight. The animals were sacrificed at 4 weeks, and the anterior and posterior halves of the medial and lateral menisci were analyzed separately. Analysis of aggrecan gene expression by quantitative polymerase chain reaction showed decreased aggrecan gene expression in menisci from immobilized knees (P < 0.01, two-way analysis of variance). Aggrecan gene expression decreased by a factor of 2 to 5.5 in the different regions examined. Analysis of the composition of the meniscus also showed decreased proteoglycan content and increased water content with immobilization (P < 0.05, two-way analysis of variance). These results show that joint immobilization can significantly affect meniscal cellular activity and composition and can therefore potentially affect meniscal function.
authors.affiliation: Department of Orthopaedic Surgery, Columbia University, New York, New York, USA.
authors.forename: M
authors.initials: M
authors.lastname: Djurasovic
authors.forename: J W
authors.initials: JW
authors.lastname: Aldridge
authors.forename: R
authors.initials: R
authors.lastname: Grumbles
authors.forename: M P
authors.initials: MP
authors.lastname: Rosenwasser
authors.forename: D
authors.initials: D
authors.lastname: Howell
authors.forename: A
authors.initials: A
authors.lastname: Ratcliffe
mesh_terms: D053674:Aggrecans; D000818:Animals; D004285:Dogs; D016326:Extracellular Matrix Proteins; D015870:Gene Expression; D007103:Immobilization / Q000009:adverse effects*; D007718:Knee Injuries / Q000628:therapy; D007719:Knee Joint / Q000502:physiology*; D037181:Lectins, C-Type; D008592:Menisci, Tibial / Q000502:physiology*; D016133:Polymerase Chain Reaction; D011509:Proteoglycans / Q000096:biosynthesis / Q000235:genetics* / Q000378:metabolism
chemical_list: D053674:Aggrecans; D016326:Extracellular Matrix Proteins; D037181:Lectins, C-Type; D011509:Proteoglycans