Susceptibility of Aspergillus strains from culture collections to amphotericin B and itraconazole.
Susceptibility testing of 27 Aspergillus reference strains belonging to five species was performed using the microdilution broth method with yeast nitrogen broth and RPMI-1640. Similar results were found using the two media. The strains of Aspergillus fumigatus (n = 8) and Aspergillus niger (n = 4) had MICs of amphotericin B in the range 0.125-0.5 mg/L. In contrast, nine out of 13 strains of Aspergillus flavus and Aspergillus nidulans had MICs in the range 2-16 mg/L. All strains had MICs of itraconazole in the range 0.03-0.5 mg/L. It is concluded that both media can be used for testing. Susceptibility testing to amphotericin B is recommended in clinical settings.
Rath
P M
PM
Institut für Medizinische Mikrobiologie, Universität-GH Essen, Germany.
D000666:Amphotericin B / Q000494:pharmacology*; D000935:Antifungal Agents / Q000494:pharmacology*; D001230:Aspergillus / Q000187:drug effects* / Q000302:isolation & purification; D006801:Humans; D017964:Itraconazole / Q000494:pharmacology*; D008826:Microbial Sensitivity Tests
D000935:Antifungal Agents; D017964:Itraconazole; D000666:Amphotericin B