title: Expression and cytogenetic localization of the human SM22 gene (TAGLN).
abstract: SM22 is a 22-kDa protein identified variously as SM22, transgelin, WS3-10, or mouse p27. Though its precise function is unknown, it is abundant in smooth muscle and so may contribute to the physiology of this widespread tissue. We found that cosmid 16b6 contains the entire 5.4-kb, five-exon human SM22 gene (HGMW-approved symbol, TAGLN), and we cytogenetically localized the gene to chromosome 11q23.2. Northern analysis of human adult tissues showed that SM22 mRNA is most prevalent in smooth muscle-containing tissues, but is also found at lower levels in heart. The human SM22 promoter contains nuclear factor-binding motifs known to regulate transcription in smooth muscle, and human SM22 promoter-luciferase reporter constructs exhibited high transcriptional activity in A7r5 or primary canine aortic smooth muscle cells, but show little activity in nonmuscle COS7 cells. In addition, human SM22 promoter activity increased by two- to threefold upon serum stimulation of nonmuscle cells.
authors.affiliation: Department of Medicine, University of Chicago, Illinois 60637, USA.
authors.forename: B
authors.initials: B
authors.lastname: Camoretti-Mercado
authors.forename: S M
authors.initials: SM
authors.lastname: Forsythe
authors.forename: M M
authors.initials: MM
authors.lastname: LeBeau
authors.forename: R
authors.initials: R
authors.lastname: Espinosa
authors.forename: J E
authors.initials: JE
authors.lastname: Vieira
authors.forename: A J
authors.initials: AJ
authors.lastname: Halayko
authors.forename: S
authors.initials: S
authors.lastname: Willadsen
authors.forename: B
authors.initials: B
authors.lastname: Kurtz
authors.forename: C
authors.initials: C
authors.lastname: Ober
authors.forename: G A
authors.initials: GA
authors.lastname: Evans
authors.forename: R
authors.initials: R
authors.lastname: Thweatt
authors.forename: S
authors.initials: S
authors.lastname: Shapiro
authors.forename: Q
authors.initials: Q
authors.lastname: Niu
authors.forename: Y
authors.initials: Y
authors.lastname: Qin
authors.forename: P A
authors.initials: PA
authors.lastname: Padrid
authors.forename: J
authors.initials: J
authors.lastname: Solway
mesh_terms: D000328:Adult; D000818:Animals; D019556:COS Cells; D002874:Chromosome Mapping; D002880:Chromosomes, Human, Pair 11; D004285:Dogs; D006801:Humans; D017403:In Situ Hybridization; D008156:Luciferases / Q000096:biosynthesis; D008214:Lymphocytes / Q000378:metabolism; D008297:Male; D051379:Mice; D008840:Microfilament Proteins; D008969:Molecular Sequence Data; D009124:Muscle Proteins / Q000096:biosynthesis / Q000235:genetics*; D009130:Muscle, Smooth / Q000378:metabolism; D009131:Muscle, Smooth, Vascular / Q000378:metabolism; D009928:Organ Specificity; D011401:Promoter Regions, Genetic; D011994:Recombinant Proteins / Q000096:biosynthesis; D014158:Transcription, Genetic; D014162:Transfection
chemical_list: D008840:Microfilament Proteins; D009124:Muscle Proteins; D011994:Recombinant Proteins; C058992:Tagln protein, mouse; C051615:transgelin; D008156:Luciferases