Development of monkey C-reactive protein (CRP) assay methods.
Monkey-specific C-reactive protein (CRP) assay methods (enzyme-linked immunosorbent assay (ELISA) and turbidimetric immunoassay (TIA)) were developed. The anti-monkey CRP serum was prepared by immunization of rabbits with the immune complex formed between the acute-phase serum from turpentine oil-inoculated monkeys and goat anti-human CRP serum. The specificity of the rabbit anti-monkey CRP serum was confirmed by immunoelectrophoresis and Western blotting. The purity of monkey CRP prepared by chromatography procedures was confirmed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE). The serum CRP levels in nine normal monkeys, as measured by sandwich ELISA were ranged from 0.26 to 1.42 microg/ml (mean 0.71+/-0.37). The CRP levels in five acute-phase sera of turpentine oil-inoculated monkeys were 248-451 microg/ml (mean 371.2+/-73.8). This monkey-specific CRP assay method was found more sensitive than the human-specific CRP assay method in detecting monkey CRP by TIA.
Jinbo
T
T
Department of Immunology, Faculty of Environmental and Health Sciences, Azabu University, Sagamihara, Kanagawa, Japan.
Hayashi
S
S
Iguchi
K
K
Shimizu
M
M
Matsumoto
T
T
Naiki
M
M
Yamamoto
S
S
D000818:Animals; D000918:Antibody Specificity; D001774:Blood Chemical Analysis / Q000379:methods / Q000662:veterinary*; D002097:C-Reactive Protein / Q000032:analysis* / Q000276:immunology; D003429:Cross Reactions; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods / Q000662:veterinary; D006801:Humans; D007118:Immunoassay / Q000379:methods / Q000662:veterinary; D008251:Macaca / Q000097:blood*; D008253:Macaca mulatta / Q000097:blood; D009391:Nephelometry and Turbidimetry / Q000379:methods / Q000662:veterinary; D011817:Rabbits; D013045:Species Specificity
D002097:C-Reactive Protein