Suitability of repetitive-DNA-sequence-based PCR fingerprinting for characterizing epidemic isolates of Salmonella enterica serovar Saintpaul.
Three molecular typing methods, repetitive-sequence-based PCR (rep-PCR) fingerprinting, plasmid profiling, and arbitrarily primed PCR fingerprinting, were used to characterize isolates of Salmonella enterica serovar Saintpaul. Most of the isolates were obtained from epidemic human cases of food-borne salmonellosis, together with some from the food material suspected to be the source of infection, and a few were obtained from other cases apparently not related to the epidemic. All three methods adequately discriminated the epidemic strain from other strains of the serovar. In addition several isolates from human cases which are not identical to the epidemic strain were found. These isolates therefore must have been responsible for some sporadic infections, which were only temporally related to the epidemic. These strains showed a high degree of similarity to a strain isolated from a turkey. rep-PCR fingerprinting with REP-Dt primers and primer ERIC1R, applicable even to crude cell lysates, offers an attractive choice as a primary method for the discrimination of various Salmonella serotypes as well as isolates within serotype Saintpaul.
Beyer
W
W
Universität Hohenheim, Institut für Umwelt- und Tierhygiene sowie Tiermedizin mit Tierklinik, Stuttgart, Germany. beyer@uni-hohenheim.de
Mukendi
F M
FM
Kimmig
P
P
Böhm
R
R
D015373:Bacterial Typing Techniques; D016172:DNA Fingerprinting / Q000379:methods*; D004269:DNA, Bacterial / Q000032:analysis; D004196:Disease Outbreaks; D005243:Feces / Q000382:microbiology; D005516:Food Microbiology; D006801:Humans; D010957:Plasmids / Q000235:genetics; D016133:Polymerase Chain Reaction / Q000379:methods*; D012091:Repetitive Sequences, Nucleic Acid; D012478:Salmonella Food Poisoning / Q000382:microbiology*; D019779:Salmonella enterica / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification; D011198:Solanum tuberosum / Q000382:microbiology
D004269:DNA, Bacterial