Recombinant cholera toxin B subunit acts as an adjuvant for the mucosal and systemic responses of mice to mucosally co-administered bovine serum albumin.
We examined the mucosal adjuvant activity of recombinant cholera toxin B subunit (rCTB) produced by Bacillus brevis carrying pNU212-CTB by intranasal or oral co-administration of bovine serum albumin (BSA). Intranasal administration stimulated a high level of BSA-specific serum IgG antibody response and BSA-specific IgA antibody responses in the nasal and pulmonary lavages. Oral administration induced a moderate level of BSA-specific serum IgG antibody and a low level of BSA-specific IgA antibody in the large intestinal washes. These results show that CTB alone can act as an intranasal or oral delivery carrier; it also has strong adjuvant properties for stimulating serum IgG and mucosal IgA immune responses to unrelated, non-coupled antigens after intranasal or oral co-immunization.
Tochikubo
K
K
Department of Microbiology, Nagoya City University, Medical School, Japan.
Isaka
M
M
Yasuda
Y
Y
Kozuka
S
S
Matano
K
K
Miura
Y
Y
Taniguchi
T
T
D000276:Adjuvants, Immunologic / Q000008:administration & dosage*; D000281:Administration, Intranasal; D000284:Administration, Oral; D000818:Animals; D000906:Antibodies / Q000032:analysis; D002772:Cholera Toxin / Q000008:administration & dosage* / Q000276:immunology; D005260:Female; D007070:Immunoglobulin A / Q000032:analysis; D007074:Immunoglobulin G / Q000032:analysis; D007413:Intestinal Mucosa / Q000276:immunology; D051379:Mice; D008807:Mice, Inbred BALB C; D009297:Nasal Mucosa / Q000276:immunology; D011994:Recombinant Proteins / Q000008:administration & dosage; D012710:Serum Albumin, Bovine / Q000008:administration & dosage* / Q000276:immunology
D000276:Adjuvants, Immunologic; D000906:Antibodies; D007070:Immunoglobulin A; D007074:Immunoglobulin G; D011994:Recombinant Proteins; D012710:Serum Albumin, Bovine; D002772:Cholera Toxin