[Determination of mutation frequency at loci of glycophorin A and T-cell receptors: informativeness for biological dosimetry of acute and prolonged irradiation].
The frequencies of somatic mutations at loci of glycophorin A (GPA) and T-cell receptor (TCR) were determined in persons exposed professionally to ionizing radiation or a result of accidents at nuclear power plants and in control donors. Dependence of glycophorin A mutant (NO) cell frequency on doses of acute (up to 3.5 Gy) and prolonged (up to 15.0 Gy) irradiation was shown. The slope of linear regression corresponded to increase of NO-mutant frequency by 31.1 x 10(-6)/Gy (r = 0.87, p < 0.0001) for acute irradiation and by 6.3 x 10(-6)/Gy (r = 0.61, p < 0.0001) for prolonged one. The 5-fold decrease of the linear regression slope in the case of prolonged irradiation makes significantly worse permissive ability of the GPA test. Therefore its use for biological dosimetry of prolonged irradiation is not expedient in dose interval up to 1 Gy. The frequency of mutations in genes of T-cell receptor significantly correlated with dose of irradiation only in group of donors with recent radiation exposure (r = 0.75, p = 0.0002). Meanwhile, the TCR method is more sensitive and informative for biological dosimetry of recent radiation, than the GPA test.
Saenko
A S
AS
Medical Radiological Research Center, Russian Academy Medical Sciences, Obninsk.
Zamulaeva
I A
IA
Smirnova
S G
SG
Orlova
N V
NV
Selivanova
E I
EI
Nugis
V Iu
VIu
Nadezhina
N M
NM
D000293:Adolescent; D000328:Adult; D000368:Aged; D003627:Data Interpretation, Statistical; D005434:Flow Cytometry; D006021:Glycophorins / Q000235:genetics* / Q000528:radiation effects; D006801:Humans; D016014:Linear Models; D008875:Middle Aged; D009154:Mutation; D009790:Occupations; D011210:Power Plants; D011829:Radiation Dosage; D011832:Radiation Injuries / Q000175:diagnosis / Q000235:genetics; D018788:Radioactive Hazard Release; D011874:Radiometry; D011948:Receptors, Antigen, T-Cell / Q000235:genetics* / Q000528:radiation effects; D012984:Software; D013997:Time Factors; D014455:Ukraine
D006021:Glycophorins; D011948:Receptors, Antigen, T-Cell
Opredelenie chastoty mutatsiĭ po lokusam glikoforina A i T-kletochnogo retseptora: informativnost' dlia biologicheskoĭ dozimetrii ostrogo i prolongirovannogo oblucheniia.