title: Structure of heparan sulfate: identification of variable and constant oligosaccharide domains in eight heparan sulfates of different origins.
abstract: The use of specific enzymes (heparinase and heparitinases from Flavobacterium heparinum, endoglucuronidase, alphaN-acetylglucosaminidase and beta-glucuronidase from the mollusc Anomalocardia brasiliana) and chemical methods (nitrous acid degradation, hydrazine N-deacetylation and borohydride reduction), led to the proposal of the total sequence of a heparan sulfate derived from bovine pancreas and partial sequences of heparan sulfates from different origins (bovine: lung, liver, brain; hog: liver, brain; rabbit liver; dog liver). It was shown that all the heparan sulfates contain common structural features such as: a N-acetylated and a N-sulfated domain made of glucuronic acid-containing disaccharides and a more sulfated region made of iduronic acid-containing disaccharides. Separating the two domains a peculiar tetrasaccharide made of GlcNAc-(alpha1-4)-IdoUA-(alpha1-4)-GlcNS-(alpha1-4)-IdoUA was identified in all the heparan sulfates analyzed. It was also shown that the non-reducing ends of the heparan sulfates contain the monosaccharides glucosamine N-sulfate and/or glucosamine 2,6 disulfate.
authors.affiliation: Departamento de Bioquimica, Escola Paulista de Medicina, UNIFESP, São Paulo, SP, Brazil.
authors.forename: C P
authors.initials: CP
authors.lastname: Dietrich
authors.forename: I L
authors.initials: IL
authors.lastname: Tersariol
authors.forename: L
authors.initials: L
authors.lastname: Toma
authors.forename: C T
authors.initials: CT
authors.lastname: Moraes
authors.forename: M A
authors.initials: MA
authors.lastname: Porcionatto
authors.forename: F W
authors.initials: FW
authors.lastname: Oliveira
authors.forename: H B
authors.initials: HB
authors.lastname: Nader
mesh_terms: D000818:Animals; D001665:Binding Sites; D002236:Carbohydrate Conformation; D002240:Carbohydrate Sequence; D002417:Cattle; D004187:Disaccharides / Q000737:chemistry; D004285:Dogs; D006497:Heparan Sulfate / Q000737:chemistry* / Q000378:metabolism; D008969:Molecular Sequence Data; D011133:Polysaccharide-Lyases / Q000378:metabolism; D011817:Rabbits; D013552:Swine
chemical_list: D004187:Disaccharides; D006497:Heparan Sulfate; D011133:Polysaccharide-Lyases; C021349:heparitinsulfate lyase