title: Antibody responses to Pasteurella haemolytica 1:A and three of its outer membrane proteins in serum, nasal secretions, and bronchoalveolar lavage fluid from calves.
abstract: OBJECTIVE
  To determine systemic and mucosal antibody responses in calves to Pasteurella haemolytica 1:A and to 2 major outer membrane proteins (OMP) and 1 major iron-regulated OMP of P haemolytica 1:A.
  
  
  METHODS
  23 crossbred calves.
  
  
  METHODS
  2 experiments were performed in the first experiment, 6 calves were vaccinated and challenge exposed intranasally with an aerosol of P haemolytica 1:A and 6 calves were only challenge exposed. In the second experiment, 8 calves were vaccinated in the area of the tracheal bifurcation with an aerosol of P haemolytica 1:A and 3 calves were used as controls. Serum, nasal secretions, and bronchoalveolar lavage (BAL) samples were collected, and IgG1, IgG2, IgA, and IgM titers were determined. Nasal secretions and BAL samples were also submitted for bacterial culture.
  
  
  RESULTS
  Serum antibody responses in the 2 groups were similar. Antibody titers in nasal secretions and BAL samples increased in calves vaccinated intranasally. In calves vaccinated in the area of the tracheal bifurcation, antibody titers increased in BAL samples but not in nasal secretions. Antibody responses did not correlate with results of bacterial culture.
  
  
  CONCLUSIONS
  Results indicated that intranasal administration of P haemolytica 1:A may be a better method for stimulating protective immune responses in the upper portion of the respiratory tract than lung administration. The single dilution ELISA provided a reliable and economical method for determining antibody titers.
authors.affiliation: Department of Veterinary Science, Louisiana State University Agricultural Center, Baton Rouge 70803, USA.
authors.forename: R E
authors.initials: RE
authors.lastname: Brennan
authors.forename: R E
authors.initials: RE
authors.lastname: Corstvet
authors.forename: D B
authors.initials: DB
authors.lastname: Paulson
mesh_terms: D000818:Animals; D000907:Antibodies, Bacterial / Q000032:analysis / Q000096:biosynthesis* / Q000097:blood; D000917:Antibody Formation; D001425:Bacterial Outer Membrane Proteins / Q000276:immunology*; D001428:Bacterial Vaccines; D001992:Bronchoalveolar Lavage Fluid / Q000276:immunology*; D002417:Cattle; D002418:Cattle Diseases / Q000276:immunology*; D007070:Immunoglobulin A / Q000096:biosynthesis; D007074:Immunoglobulin G / Q000096:biosynthesis; D007075:Immunoglobulin M / Q000096:biosynthesis; D016978:Mannheimia haemolytica / Q000276:immunology*; D009297:Nasal Mucosa / Q000276:immunology* / Q000378:metabolism; D010326:Pasteurella Infections / Q000276:immunology / Q000662:veterinary*
chemical_list: D000907:Antibodies, Bacterial; D001425:Bacterial Outer Membrane Proteins; D001428:Bacterial Vaccines; D007070:Immunoglobulin A; D007074:Immunoglobulin G; D007075:Immunoglobulin M