High-throughput DNA sequencing on a capillary array electrophoresis system.
A capillary array electrophoresis apparatus capable of running and analyzing 48 DNA sequencing samples simultaneously has been constructed. The instrument uses a replaceable sieving buffer and incorporates a convenient method for introducing the buffer into the capillaries. Data from laser-induced fluorescence are collected as four separate images, one for each optical channel. The integrated data analysis software employs an open architecture that allows use of any DNA base-calling algorithm. DNA sequencing runs are completed in approx. 1 hr (approximately 500 bases), and instrument turnaround time between runs is less than 15 min. Overall, the instrument throughput is on the order of 720 templates/day, or 360,000 bases/day.
Marsh
M
M
Molecular Dynamics, Sunnyvale, CA, USA.
Tu
O
O
Dolnik
V
V
Roach
D
D
Solomon
N
N
Bechtol
K
K
Smietana
P
P
Wang
L
L
Li
X
X
Cartwright
P
P
Marks
A
A
Barker
D
D
Harris
D
D
Bashkin
J
J
D000818:Animals; D019075:Electrophoresis, Capillary / Q000295:instrumentation*; D006801:Humans; D017422:Sequence Analysis, DNA / Q000295:instrumentation* / Q000379:methods*