[Genetic analysis of Saccharomyces castellii, S. exiguus and S. martiniae yeasts].
Genetic lines of three biological yeast species of the Saccharomyces sensu lato group--S. castellii, S. exiguus, and S. martiniae--were created. Homo- and heterothalmic monosporic fertile strains were marked by different auxotrophic mutations. Conditions for the hybridization of the Saccharomyces sensu lato yeast were established. The mating of spores or haploid cells of auxotrophic complementary mutants was performed on the complete YPD medium. Prototrophic diploid hybrids were selected on a minimal medium. In S. castellii, the hybridization of diploid cells was demonstrated. All three species studied were suitable for tetrad analysis.
Naumov
G I
GI
State Research Institute of Genetics and Selection of Industrial Microorganisms, Moscow, Russia. gennadi@vnigen.msk.su
Naumova
E S
ES
Marinoni
G
G
Piskur
J
J
D004271:DNA, Fungal / Q000235:genetics; D006824:Hybridization, Genetic; D012440:Saccharomyces / Q000235:genetics*; D013045:Species Specificity
D004271:DNA, Fungal
Geneticheskiĭ analiz drozhzheĭ Saccharomyces casteillii, S. exiguus iS. martiniae.