[Developing methods of specific heterologic immunoglobulins preparation for urgent prevention of Ebola fever and study of their properties].
Methods for preventing and treating Ebola virus hemorrhagic fever are not still available despite the fact that this virus have been studied for 20 years. Methods of immunization of the animals (sheep, goats) non-susceptible to Ebola virus with live virus preparations were developed to obtain the hyperimmune anti-Ebola virus sera required to have highly immune antivirus gamma-globulins. These methods made it possible to obtain the immune sera having high virus-neutralizing antibodies. Caprine immunoglobulins were obtained from sera by fractionation of immune sera by Kohn's method. The neutralization indices of the immunoglobulins obtained were at least Ig. When administered in the first hours of infection, the protective effect of these preparations was shown on guinea pigs infected with LD50 of the strain pathogenic to the animals. Preclinical trials of these immunoglobulins on laboratory animals and clinical trials on volunteers were performed. The preparation was used as a preventive agent when accidents took place at the laboratory working with Ebola virus. The similar preparation from equine sera having high neutralizing and protective properties was elaborated at the Virological Center, Microbiological Institute, Russian Ministry of Defense. Its prophylactic efficiency was also shown in infected gamadrias.
Cherpunov
A A
AA
Kudoiarova-Zubavichene
N M
NM
Dedkova
L M
LM
Sergeev
N N
NN
Netesov
S V
SV
D000818:Animals; D000914:Antibodies, Viral / Q000008:administration & dosage*; D004339:Drug Compounding; D029043:Ebolavirus / Q000276:immunology* / Q000472:pathogenicity; D004630:Emergencies; D005500:Follow-Up Studies; D006041:Goats; D006168:Guinea Pigs; D000882:Haplorhini; D019142:Hemorrhagic Fever, Ebola / Q000276:immunology / Q000517:prevention & control* / Q000821:virology; D006736:Horses; D006801:Humans; D007074:Immunoglobulin G / Q000008:administration & dosage*; D051379:Mice; D011817:Rabbits; D012756:Sheep
D000914:Antibodies, Viral; D007074:Immunoglobulin G
Razrabotka metodov polucheniia spetsificheskikh geterologichnykh immunoglobulinov dlia ékstrennoĭ profilaktiki likhoradki ébola i izuchenie ikh svoĭstv.