Determination of the enantiomers of chlorpheniramine and its main monodesmethyl metabolite in urine using achiral-chiral liquid chromatography.
The enantiomers of chlorpheniramine and its monodesmethyl metabolite were determined separately in urine by using a coupled achiral-chiral chromatographic system. The two enantiomers of the studied compound and the internal standard were separated from the biological matrix on a cyanopropyl column and reinjected into a chiral amylose AD column where the two enantiomers were separated and quantified by UV detection. The method was validated for chlorpheniramine and for the metabolite within the range 0-1000 ng/ml. It was also applied in a pilot pharmacokinetic study to samples from a volunteer given 8 mg of racemic chlorpheniramine by mouth.
Hiep
B T
BT
Pitié Salpêtrière Hospital, Service Pharmcie, Paris, France.
Khanh
V
V
Hung
N K
NK
Thuillier
A
A
Gimenez
F
F
D001711:Biotransformation; D002744:Chlorpheniramine / Q000031:analogs & derivatives* / Q000493:pharmacokinetics / Q000652:urine; D002853:Chromatography, Liquid; D006634:Histamine H1 Antagonists / Q000493:pharmacokinetics / Q000652:urine*; D006801:Humans; D015203:Reproducibility of Results; D012997:Solvents; D013056:Spectrophotometry, Ultraviolet; D013237:Stereoisomerism
D006634:Histamine H1 Antagonists; D012997:Solvents; C041905:N-desmethylchlorpheniramine; D002744:Chlorpheniramine