title: CXCR4 and CCR5 genetic polymorphisms in long-term nonprogressive human immunodeficiency virus infection: lack of association with mutations other than CCR5-Delta32.
abstract: Polymorphisms in the coding sequences of CCR5 and CXCR4 were studied in a group of human immunodeficiency virus (HIV)-infected long-term nonprogressors. Two different point mutations were found in the CXCR4 coding sequence. One of these CXCR4 mutations was silent, and each was unique to two nonprogressors. The well-described 32-bp deletion within the CCR5 coding sequence (CCR5-Delta32) was found in 4 of 13 nonprogressors, and 12 different point mutations were found scattered over the CCR5 coding sequence from 8 nonprogressors. Most of the mutations created either silent or conservative changes in the predicted amino acid sequence: only one of these mutations was found in more than a single nonprogressor. All nonsilent mutations were tested in an HIV envelope-dependent fusion assay, and all functioned comparably to wild-type controls. Polymorphisms in the CXCR4 and CCR5 coding sequences other than CCR5-Delta32 do not appear to play a dominant mechanistic role in nonprogression among HIV-infected individuals.
authors.affiliation: Laboratory of Immunoregulation, National Institute of Allergy and Infectious Diseases, Bethesda, Maryland 20892-1876, USA. OCohen@nih.gov
authors.forename: O J
authors.initials: OJ
authors.lastname: Cohen
authors.forename: S
authors.initials: S
authors.lastname: Paolucci
authors.forename: S M
authors.initials: SM
authors.lastname: Bende
authors.forename: M
authors.initials: M
authors.lastname: Daucher
authors.forename: H
authors.initials: H
authors.lastname: Moriuchi
authors.forename: M
authors.initials: M
authors.lastname: Moriuchi
authors.forename: C
authors.initials: C
authors.lastname: Cicala
authors.forename: R T
authors.initials: RT
authors.lastname: Davey
authors.forename: B
authors.initials: B
authors.lastname: Baird
authors.forename: A S
authors.initials: AS
authors.lastname: Fauci
mesh_terms: D005260:Female; D015658:HIV Infections / Q000235:genetics*; D006801:Humans; D008297:Male; D009154:Mutation; D011110:Polymorphism, Genetic; D019713:Receptors, CCR5 / Q000235:genetics*; D019718:Receptors, CXCR4 / Q000235:genetics*
chemical_list: D019713:Receptors, CCR5; D019718:Receptors, CXCR4