title: Endotoxin removal with poly(ethyleneimine)-immobilized adsorbers: Sepharose 4B versus flat sheet and hollow fibre membranes.
abstract: Poly(ethyleneimine) was immobilized on poly(vinyl alcohol)-coated nylon flat sheet membranes, poly(vinyl alcohol) and poly(ethylenevinyl alcohol) hollow fibre membranes as well as Sepharose 4B. The resulting poly(ethyleneimine)-immobilized adsorbers were used for removal of E. coli derived endotoxin from buffers and bovine serum albumin solutions. The efficiency of poly(ethyleneimine) proved to be constant over a wide pH range, including phosphate buffered saline. The performance depended upon the matrix type employed: endotoxin clearance factors varied from 100 to 120,000 in protein-free solutions and 40 to 33,000 in solutions of bovine serum albumin using 6000 EU/ml as feed concentration. The best adsorber was the flat sheet membrane-immobilized poly(ethyleneimine), followed by the hollow fibre-immobilized poly(ethyleneimine) and poly(ethyleneimine)-Sepharose. The factors influencing endotoxin clearance were the mass transport (convective systems were superior to the diffusive system), the chemical composition and the surface structure of the underlying matrix.
authors.affiliation: GBF, Gesellschaft für Biotechnologische Forschung mbH, Biochemical Engineering Division, Braunschweig, Germany.
authors.forename: D
authors.initials: D
authors.lastname: Petsch
authors.forename: W D
authors.initials: WD
authors.lastname: Deckwer
authors.forename: F B
authors.initials: FB
authors.lastname: Anspach
authors.forename: C
authors.initials: C
authors.lastname: Legallais
authors.forename: M
authors.initials: M
authors.lastname: Vijayalakshmi
mesh_terms: D000042:Absorption; D002021:Buffers; D004731:Endotoxins / Q000302:isolation & purification*; D004926:Escherichia coli / Q000737:chemistry*; D006863:Hydrogen-Ion Concentration; D008024:Ligands; D008567:Membranes, Artificial; D008628:Mercury; D011094:Polyethyleneimine; D012685:Sepharose; D012710:Serum Albumin, Bovine / Q000737:chemistry; D012996:Solutions
chemical_list: D002021:Buffers; D004731:Endotoxins; D008024:Ligands; D008567:Membranes, Artificial; D012996:Solutions; D012710:Serum Albumin, Bovine; C044101:endotoxin, Escherichia coli; D011094:Polyethyleneimine; D012685:Sepharose; D008628:Mercury