[Multiple antibiotic resistance mediated by R plasmid in Shigella flexneri strains isolated in the northeast of Brazil].
In Shigella strains were studied the molecular mechanism that mediated the multiply antibiotic-resistance. Twenty-six strains of Shigella flexneri were utilised in this investigation. These strains were submitted to disk diffusion test, mating experiments and plasmid isolation. In relation to antibiotics resistance standard it was observed that all Shigella flexneri strains were resistant to at least, three antibiotics tested. From twenty-six Shigella flexneri strains donors submitted to conjugation process, 34.6% (nine strains) resulted in variable frequency of transconjugants. From strains that conjugated, 100%, transferred the resistance factor acquainted with ampicillin. Being that, in all transconjugants which were observed, just one plasmid with 23.1 Kb was evidenced. This plasmid found in all strains was characterised as the cause of resistance to ampicillin.
Sidrim
J J
JJ
Departamento de Patologia, Hospital Universitário Walter Cantídio, Fortaleza, Brasil.
Moreira
J L
JL
Paixão
G C
GC
Lima
S B
SB
Filho
R E
RE
Rocha
M F
MF
Lima
A A
AA
D000668:Ampicillin Resistance; D001938:Brazil; D004352:Drug Resistance, Microbial; D018432:Drug Resistance, Multiple; D008826:Microbial Sensitivity Tests; D011815:R Factors; D012763:Shigella flexneri / Q000187:drug effects*
Multirresistência a antimicrobianos mediada por plasmídios R em cepas de Shigella flexneri isoladas no nordeste do Brasil.