Development of an antigen presentation system based on plum pox potyvirus.
The development of an antigen presentation system based on the plum pox potyvirus (PPV) is here described. The amino-terminal part of PPV capsid protein was chosen as the site for expression of foreign antigenic peptides. Modifications in this site were engineered to avoid the capability of natural transmission by aphids of this PPV vector. As a first practical attempt, different forms of an antigenic peptide (single and tandem repetition) from the VP2 capsid protein of canine parvovirus (CPV) were expressed. Both chimeras are able to infect Nicotiana clevelandii plants with similar characteristics to wild-type virus and remain genetically stable after several plant passages. The antigenicity of purified chimeric virions was demonstrated, proving the suitability of this system for diagnostic purposes. Moreover, mice and rabbits immunized with chimeric virions developed CPV-specific antibodies, which showed neutralizing activity.
Fernández-Fernández
M R
MR
Centro Nacional de Biotecnología (CNB-CSIC), Campus de la Universidad Autónoma de Madrid, Spain. mrfdez@cnb.uam.es
Martínez-Torrecuadrada
J L
JL
Casal
J I
JI
García
J A
JA
D000595:Amino Acid Sequence; D000818:Animals; D000911:Antibodies, Monoclonal / Q000096:biosynthesis; D000914:Antibodies, Viral / Q000096:biosynthesis; D017951:Antigen Presentation; D002213:Capsid / Q000235:genetics / Q000276:immunology; D036022:Capsid Proteins; D003001:Cloning, Molecular / Q000379:methods; D000939:Epitopes / Q000235:genetics; D005822:Genetic Vectors; D051379:Mice; D008807:Mice, Inbred BALB C; D008969:Molecular Sequence Data; D009500:Neutralization Tests; D017993:Parvovirus, Canine / Q000235:genetics / Q000276:immunology*; D010455:Peptides / Q000276:immunology; D017801:Plum Pox Virus / Q000235:genetics* / Q000276:immunology; D011817:Rabbits; D011993:Recombinant Fusion Proteins; D017384:Sequence Deletion; D014614:Vaccines, Synthetic / Q000235:genetics* / Q000276:immunology; D014765:Viral Vaccines / Q000235:genetics* / Q000276:immunology
D000911:Antibodies, Monoclonal; D000914:Antibodies, Viral; D036022:Capsid Proteins; D000939:Epitopes; D010455:Peptides; D011993:Recombinant Fusion Proteins; D014614:Vaccines, Synthetic; D014765:Viral Vaccines; C095320:coat protein, Plum pox virus