title: p53 and cyclinX D1 protein expression in carcinomas of the parotid gland.
abstract: BACKGROUND
  p53 and cyclin D1 genes play a central role in the regulation of the G1 phase of the cell-cycle, and are frequently involved in head and neck tumorigenesis.
  
  
  METHODS
  By means of immunohistochemistry, we retrospectively investigated the overexpression of cyclin D1 and p53 genes in a series of 28 parotid gland carcinomas. The immunohistochemical analysis was performed using the ABC method and the antibodies DCS6 (for cyclin D1) and CM1 (for p53).
  
  
  RESULTS
  p53 was overexpressed in 12 (42.9%) and cyclin D1 in 6 cases (21.4%). No significant association was found between p53 or cyclin D1 expression and the evaluated clinicopathological parameters of tumor extension, clinical stage, and lymph node or distant metastases. Nevertheless, it is worth noting that all of the patients with a high expression of p53 died of their disease.
  
  
  CONCLUSIONS
  The present data confirm the role of p53 abnormalities in the pathogenesis of salivary gland carcinoma and report, for the first time, the involvement of cyclin D1 gene in these tumors.
authors.affiliation: I Clinica Otorinolaringoiatrica, Ospedale Maggiore I.R.C.C.S., Milano, Italy.
authors.forename: L
authors.initials: L
authors.lastname: Pignataro
authors.forename: P
authors.initials: P
authors.lastname: Capaccio
authors.forename: N
authors.initials: N
authors.lastname: Carboni
authors.forename: G
authors.initials: G
authors.lastname: Pruneri
authors.forename: A
authors.initials: A
authors.lastname: Ottaviani
authors.forename: R
authors.initials: R
authors.lastname: Buffa
authors.forename: G
authors.initials: G
authors.lastname: Broich
mesh_terms: D000328:Adult; D000368:Aged; D002277:Carcinoma / Q000235:genetics*; D005260:Female; D015870:Gene Expression; D019940:Genes, bcl-1; D016158:Genes, p53; D006801:Humans; D007150:Immunohistochemistry; D008297:Male; D008875:Middle Aged; D009362:Neoplasm Metastasis; D010307:Parotid Neoplasms / Q000235:genetics* / Q000473:pathology; D012189:Retrospective Studies