Retinal cytokine response in mouse alkali-burned eye.
The kinetics of inflammatory cytokines in sensory retina of mice with corneal alkali-burned eyes were examined. Retinal samples were collected from ICR mice with unburned and alkali-burned eyes from day 3 to day 14. Enzyme-linked immunosorbent assay was used to quantitate the expression levels of interleukin-1 alpha (IL-1 alpha), IL-1 beta and IL-6. Concentrations of IL-1 beta and IL-6 were significantly elevated at day 3 after alkali burn, and reduced to normal level by day 7. The concentration of IL-1 alpha was below assay sensitivity at all times. A significant elevation of IL-1 beta and IL-6 in the sensory retina may contribute to the retinal inflammatory response in the alkali-burned eyes. This is the first report suggesting that the retina is involved in the damage process of alkali burn.
Miyamoto
F
F
Department of Ophthalmology, Kyoto Prefectural University of Medicine, Japan.
Sotozono
C
C
Ikeda
T
T
Kinoshita
S
S
D000818:Animals; D002057:Burns, Chemical / Q000209:etiology*; D003315:Cornea / Q000187:drug effects; D065306:Corneal Injuries; D004797:Enzyme-Linked Immunosorbent Assay; D005126:Eye Burns / Q000139:chemically induced*; D005260:Female; D007375:Interleukin-1 / Q000378:metabolism*; D015850:Interleukin-6 / Q000378:metabolism*; D051379:Mice; D008813:Mice, Inbred ICR; D012160:Retina / Q000378:metabolism*; D012972:Sodium Hydroxide
D007375:Interleukin-1; D015850:Interleukin-6; D012972:Sodium Hydroxide