title: A survey of amoebic infection in the Wonji area of central Ethiopia.
abstract: An epidemiological survey to characterize Entamoeba histolytica/E dispar isolates from 123 human subjects was carried out in the Wonji area of Central Ethiopia, where an increased incidence of amoebic infection has been reported. In a randomized, coproparasitological study, 93 (75.6%) of the subjects were found positive for at least one species of intestinal parasite: 14 (15.1%) harboured only one species and 79 (84.9%) were infected with at least two species. In-vitro culture in Robinson's medium revealed amoebic parasites in 52 (82.5%) of the 63 cases tested. Of the 29 amoebic isolates successfully stabilized, cloned and characterized by Sargeaunt's electrophoretic technique, 27 (93.1%) were of E. dispar zymodemes (19 of zymodeme I, two each of zymodemes III, V and XI, and one each of zymodemes X and XV) and two (6.9%) were of E. histolytica (zymodeme XIII).
authors.affiliation: Laboratory of Parasitology, Virology Service, IRCCS San Matteo, Pavia, Italy.
authors.forename: S
authors.initials: S
authors.lastname: Gatti
authors.forename: R
authors.initials: R
authors.lastname: Mahdi
authors.forename: A
authors.initials: A
authors.lastname: Bruno
authors.forename: C
authors.initials: C
authors.lastname: Cevini
authors.forename: M
authors.initials: M
authors.lastname: Scaglia
mesh_terms: D000293:Adolescent; D000328:Adult; D000818:Animals; D002648:Child; D004796:Clinical Enzyme Tests; D004748:Entamoeba histolytica / Q000145:classification; D004749:Entamoebiasis / Q000201:enzymology / Q000453:epidemiology*; D005002:Ethiopia / Q000453:epidemiology; D005243:Feces / Q000469:parasitology; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged