[Incorporation of neoglycolipids into K562 cells. Model for the study of carbohydrate dependent lysis of target cells by natural killer cells].
A model system was developed to study the role of cell surface oligosaccharides in cytotoxicity mediated by natural killer (NK) cells. Polymeric glycoconjugates Glyc-PAA-PE and Glyc-PAA(Flu)-PE (where Glyc is the 3-aminopropyl Le(x) residue, PAA is the poly(N-2-hydroxyethylacrylamide) matrix, Flu is the residue of a fluorescein derivative, and PE is the phosphatidylethanolamine residue) were synthesized, and their association with the membranes of living K562 cells was studied.
Kovalenko
E I
EI
Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Moscow, Russia.
Sablina
M A
MA
Khaĭdukov
S V
SV
Khirova
E V
EV
Bovin
N V
NV
D002462:Cell Membrane / Q000378:metabolism; D003601:Cytotoxicity Tests, Immunologic; D005434:Flow Cytometry; D019793:Fluorescein / Q000737:chemistry; D006017:Glycolipids / Q000737:chemistry / Q000378:metabolism*; D006801:Humans; D007694:Killer Cells, Natural / Q000378:metabolism*; D016256:Lewis X Antigen / Q000378:metabolism*; D009844:Oligosaccharides / Q000737:chemistry / Q000378:metabolism*; D010714:Phosphatidylethanolamines / Q000737:chemistry; D014407:Tumor Cells, Cultured
D006017:Glycolipids; D016256:Lewis X Antigen; D009844:Oligosaccharides; D010714:Phosphatidylethanolamines; D019793:Fluorescein
Vstraivanie neoglikolipidov v kletki K562. Model' dlia izucheniia uglevodozavisimogo lizisa kletok-misheneĭ estestvennymi killerami.