A molecular view of microbial diversity in a dynamic landfill in Québec.
An Aroclor 1260 (polychlorinated biphenyl, PCB)-laden soil and one heavily contaminated with polycyclic aromatic hydrocarbons (PAHs) from a secure, engineered landfill site in Québec were analyzed for microbial diversity using a clone library of the 16S rDNA sequences. Phylogenetic analysis revealed that three phyla and their major subdivisions of the domain Bacteria were highly represented in these samples despite the high pollution, particularly by PAHs. None of the 16S rDNA sequences obtained matched known sequences from cultivated bacterial species or from 16S rDNA sequences amplified directly from other environmental samples.
Lloyd-Jones
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Environmental Biotechnology Sector, Biotechnology Research Institute, National Research Council Canada, Montréal, Canada.
Lau
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PC
D004269:DNA, Bacterial / Q000235:genetics / Q000302:isolation & purification*; D004275:DNA, Ribosomal / Q000235:genetics / Q000302:isolation & purification*; D006841:Hydrocarbons, Aromatic; D010802:Phylogeny; D011792:Quebec; D017421:Sequence Analysis; D012988:Soil Microbiology; D012989:Soil Pollutants
D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D006841:Hydrocarbons, Aromatic; D012989:Soil Pollutants