Direct and continuous electrochemical measurement of noradrenaline-induced nitric oxide production in C6 glioma cells.
1. Nitric oxide (NO) production in C6 glioma cells was directly monitored in real time by electrochemical detection with a NO-specific biosensor. 2. We present here the first direct evidence that noradrenaline elicits long-lasting NO production in C6 cells pretreated with lipopolysaccharide and interferon-gamma, an effect blocked by NG-monomethyl-L-arginine, a NO synthase inhibitor. 3. This direct electrochemical measurement of glia-derived NO should facilitate our understanding of the kinetics of glial signaling in glia-glia and glia-neuron networks in the brain.
Trevin
S
S
ASTEC Head Office, Fukuoka, Japan.
Kataoka
Y
Y
Kawachi
R
R
Shuto
H
H
Kumakura
K
K
Oishi
R
R
D000818:Animals; D001120:Arginine / Q000494:pharmacology; D015374:Biosensing Techniques; D001921:Brain / Q000502:physiology; D004563:Electrochemistry / Q000379:methods; D005910:Glioma; D007371:Interferon-gamma / Q000494:pharmacology; D008070:Lipopolysaccharides / Q000494:pharmacology; D009474:Neurons / Q000502:physiology; D009569:Nitric Oxide / Q000032:analysis / Q000096:biosynthesis*; D009638:Norepinephrine / Q000494:pharmacology*; D015398:Signal Transduction; D014407:Tumor Cells, Cultured; D019323:omega-N-Methylarginine / Q000494:pharmacology
D008070:Lipopolysaccharides; D019323:omega-N-Methylarginine; D009569:Nitric Oxide; D007371:Interferon-gamma; D001120:Arginine; D009638:Norepinephrine