title: Streptomyces griseus protease B: secretion correlates with the length of the propeptide.
abstract: Streptomyces griseus protease B, a member of the chymotrypsin superfamily, is encoded by a gene that express a pre-pro-mature protein. During secretion the precursor protein is processed into a mature, fully folded protease. In this study, we constructed a family of genes which encode deletions at the amino-terminal end of the propeptide. The secretion of active protease B was seen to decrease in an exponential manner according to the length of the deletion. The results underscore the intimate relationship between folding and secretion in bacterial protease expression. They further suggest that the propeptide segment of the zymogen stabilizes the folding of the mature through many small binding interactions over the entire surface of the peptide rather than through a few specific contacts.
authors.affiliation: Department of Chemistry, Simon Fraser University, Burnaby, British Columbia, Canada.
authors.forename: J
authors.initials: J
authors.lastname: Baardsnes
authors.forename: S
authors.initials: S
authors.lastname: Sidhu
authors.forename: A
authors.initials: A
authors.lastname: MacLeod
authors.forename: J
authors.initials: J
authors.lastname: Elliott
authors.forename: D
authors.initials: D
authors.lastname: Morden
authors.forename: J
authors.initials: J
authors.lastname: Watson
authors.forename: T
authors.initials: T
authors.lastname: Borgford
mesh_terms: D001665:Binding Sites; D004792:Enzyme Precursors / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D005798:Genes, Bacterial; D008956:Models, Chemical; D017510:Protein Folding; D011499:Protein Processing, Post-Translational; D011993:Recombinant Fusion Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D017384:Sequence Deletion; D012697:Serine Endopeptidases / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D013305:Streptomyces griseus / Q000201:enzymology* / Q000235:genetics
chemical_list: D004792:Enzyme Precursors; D011993:Recombinant Fusion Proteins; D012697:Serine Endopeptidases; C024984:streptogrisin B