5'-heterogeneity of the human excitatory amino acid transporter cDNA EAAT2 (GLT-1).
TWO novel transcripts of the human excitatory amino acid transporter EAAT2 (GLT-1) were cloned using PCR cloning techniques. Comparative sequence analysis of the 5'-untranslated region (UTR) of five EAAT2 transcripts led to the definition of five putative 5'-untranslated exons that are combined in a variable manner. Alternative splicing is a likely mechanism for the 5' complexity of the EAAT2 cDNA. The potential functional meaning of this finding such as differential expression of the EAAT2 transcripts in the central nervous system remains to be demonstrated.
Münch
C
C
Department of Neurology, University of Ulm, Germany.
Schwalenstöcker
B
B
Knappenberger
B
B
Liebau
S
S
Völkel
H
H
Ludolph
A C
AC
Meyer
T
T
D018528:ATP-Binding Cassette Transporters / Q000096:biosynthesis* / Q000235:genetics; D017398:Alternative Splicing; D027322:Amino Acid Transport System X-AG; D001692:Biological Transport; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000378:metabolism; D005091:Exons; D006801:Humans; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000096:biosynthesis; D011994:Recombinant Proteins / Q000096:biosynthesis; D014158:Transcription, Genetic
D018528:ATP-Binding Cassette Transporters; D027322:Amino Acid Transport System X-AG; D018076:DNA, Complementary; D012333:RNA, Messenger; D011994:Recombinant Proteins