A role for carbohydrates in immune evasion in AIDS.
Rhesus monkeys were infected with mutant forms of simian immunodeficiency virus lacking dual combinations of the 4th, 5th and 6th sites for N-linked glycosylation in the external envelope glycoprotein of the virus. When compared with sera from monkeys infected with the parental virus, sera from monkeys infected with the mutant viruses exhibited markedly increased antibody binding to specific peptides from this region and markedly increased neutralizing activity. These results demonstrate a role for N-linked glycosylation in limiting the neutralizing antibody response to SIV and in shielding the virus from immune recognition.
Reitter
J N
JN
New England Regional Primate Research Center, Harvard Medical School, Southborough, Massachusetts 01772-9102, USA.
Means
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Desrosiers
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D000595:Amino Acid Sequence; D000818:Animals; D000914:Antibodies, Viral / Q000276:immunology / Q000378:metabolism; D000917:Antibody Formation; D001665:Binding Sites / Q000235:genetics; D002241:Carbohydrates / Q000502:physiology*; D014644:Genetic Variation; D015699:HIV Envelope Protein gp120 / Q000235:genetics / Q000276:immunology; D007114:Immunization; D008253:Macaca mulatta / Q000235:genetics / Q000276:immunology / Q000821:virology*; D008562:Membrane Glycoproteins; D008969:Molecular Sequence Data; D009154:Mutation / Q000235:genetics; D009500:Neutralization Tests; D017421:Sequence Analysis; D017386:Sequence Homology, Amino Acid; D016097:Simian Acquired Immunodeficiency Syndrome / Q000276:immunology* / Q000503:physiopathology / Q000821:virology*; D015302:Simian Immunodeficiency Virus / Q000737:chemistry / Q000235:genetics / Q000276:immunology; D014759:Viral Envelope Proteins; D019562:Viral Load
D000914:Antibodies, Viral; D002241:Carbohydrates; D015699:HIV Envelope Protein gp120; D008562:Membrane Glycoproteins; D014759:Viral Envelope Proteins; C069623:gp120 protein, Simian immunodeficiency virus