Involvement of MMP-1 and MMP-3 in collagen degradation induced by IL-1 in rabbit cartilage explant culture.
To determine whether matrix metalloproteinase-1 (MMP-1) or MMP-3 is involved in cartilage collagen degradation, polyclonal antibodies were separately raised against MMP-1 and MMP-3 and their effects on collagen degradation were assessed in rabbit cartilage explant culture. We found that anti-MMP-1 antibodies completely inhibited collagen degradation induced by the combination of interleukin-1 (IL-1) and plasminogen. Anti-MMP-3 antibodies showed 40% inhibition at maximum concentration. These results indicate that MMP-1, and possibly MMP-3, are involved in collagen degradation in cartilage explant culture.
Saito
S
S
Institute for Drug Discovery Research, Yamanouchi Pharmaceutical Co., Ltd., Tsukuba, Ibaraki, Japan.
Katoh
M
M
Masumoto
M
M
Matsumoto
S
S
Masuho
Y
Y
D000818:Animals; D002356:Cartilage / Q000187:drug effects / Q000378:metabolism*; D003094:Collagen / Q000187:drug effects / Q000378:metabolism*; D017364:Collagenases / Q000276:immunology / Q000378:metabolism*; D046508:Culture Techniques; D004338:Drug Combinations; D004797:Enzyme-Linked Immunosorbent Assay; D005620:Freund's Adjuvant; D007375:Interleukin-1 / Q000494:pharmacology*; D020781:Matrix Metalloproteinase 1; D019278:Matrix Metalloproteinase 3 / Q000276:immunology / Q000378:metabolism*; D051379:Mice; D010958:Plasminogen / Q000494:pharmacology; D011817:Rabbits
D004338:Drug Combinations; D007375:Interleukin-1; D010958:Plasminogen; D003094:Collagen; D005620:Freund's Adjuvant; D017364:Collagenases; D019278:Matrix Metalloproteinase 3; D020781:Matrix Metalloproteinase 1