title: Susceptibility to Salmonella typhimurium infection and effectiveness of vaccination in mice deficient in the tumor necrosis factor alpha p55 receptor.
abstract: Mice defective in the ability to produce the tumor necrosis factor alpha p55 receptor (TNFalphap55R) were orally challenged with a number of Salmonella typhimurium HWSH derivatives that differ in virulence. In comparison to TNFalphap55R+/+ mice, TNFalphap55R-/- mice succumbed earlier to challenge with wild-type S. typhimurium HWSH and S. typhimurium HWSH purE. In contrast, TNFalphap55R-/- mice were able to control an S. typhimurium HWSH aroA challenge, although greater numbers of Salmonella organisms were present in the tissues for a longer time period than was observed with TNFalphap55R+/+ mice. Vaccination of normal and TNFalphap55R knockout animals with S. typhimurium HWSH aroA showed that TNFalphap55R-/- mice, unlike TNFalphap55R+/+ mice, were not protected against a virulent S. typhimurium HWSH challenge. Splenocytes from TNFalphap55R-/- mice exhibited a reduced ability to proliferate in the presence of S. typhimurium antigen compared to TNFalphap55R+/+ mice. Thus, TNFalphap55R is essential for controlling Salmonella growth in tissues and for recall of immunity in murine salmonellosis.
authors.affiliation: Department of Biochemistry, Imperial College of Science, Technology and Medicine, London SW7 2AZ, United Kingdom.
authors.forename: P
authors.initials: P
authors.lastname: Everest
authors.forename: M
authors.initials: M
authors.lastname: Roberts
authors.forename: G
authors.initials: G
authors.lastname: Dougan
mesh_terms: D000818:Animals; D000907:Antibodies, Bacterial / Q000097:blood; D015169:Colony Count, Microbial; D016207:Cytokines / Q000096:biosynthesis; D004198:Disease Susceptibility; D008213:Lymphocyte Activation; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D009569:Nitric Oxide / Q000097:blood; D018124:Receptors, Tumor Necrosis Factor / Q000172:deficiency / Q000502:physiology*; D012481:Salmonella Infections, Animal / Q000276:immunology* / Q000382:microbiology / Q000473:pathology; D012486:Salmonella typhimurium / Q000302:isolation & purification; D014611:Vaccination
chemical_list: D000907:Antibodies, Bacterial; D016207:Cytokines; D018124:Receptors, Tumor Necrosis Factor; D009569:Nitric Oxide