title: Study of elastic fiber organization by scanning force microscopy.
abstract: Elastic fibers of beef ligamentum nuchae were observed by atomic force microscopy and data compared with those obtained by conventional and freeze-fracture electron microscopy. Fresh isolated elastin fibers as well as thin sections of ligament fragments, which were fixed and embedded either in relaxed or in stretched conditions, were analysed. The results confirm that, at least in beef ligamentum nuchae, elastic fibers consist of beaded filaments which can be oriented by stretching in the direction of the force applied. Moreover, atomic force microscopy revealed that these beaded filaments are laterally connected by periodical bridges which become more pronounced upon stretching. The data clearly show that elastin molecules are organized in a rather ordered array, at least at the super-molecular level, and a depiction of the elastin organization in beef ligamentum nuchae is attempted.
authors.affiliation: Biomedical Sciences Department, University of Modena, Italy.
authors.forename: I
authors.initials: I
authors.lastname: Pasquali Ronchetti
authors.forename: A
authors.initials: A
authors.lastname: Alessandrini
authors.forename: M
authors.initials: M
authors.lastname: Baccarani Contri
authors.forename: C
authors.initials: C
authors.lastname: Fornieri
authors.forename: G
authors.initials: G
authors.lastname: Mori
authors.forename: D
authors.initials: D
authors.lastname: Quaglino
authors.forename: U
authors.initials: U
authors.lastname: Valdrè
mesh_terms: D000818:Animals; D002417:Cattle; D004547:Elastic Tissue / Q000648:ultrastructure*; D005614:Freeze Fracturing; D008022:Ligaments / Q000648:ultrastructure*; D018625:Microscopy, Atomic Force; D008854:Microscopy, Electron