Glucose-6-phosphatase activity in the hypothalamus of the ob/ob mouse.
The hypothalamus and cortex from ob/ob mice and their lean littermates were sonicated and then incubated with glucose-6-phosphate (glucose-6-P) and glycerol phosphate (glycerol-P). The difference between the rates of hydrolysis of glucose-6-P and glycerol-P was taken as the measure of glucose-6-phosphatase activity. The activity was much higher in the hypothalamus from ob/ob mice versus their lean littermates. Activity was undetected in the cortex. These findings raise the possibility that a defect in the regulation of glucose-6-phosphatase activity in a portion of the hypothalamus may relate to the mechanism underlying obesity in the ob/ob mouse. However, obese gene product administration to ob/ob mice, while reducing the body weight, did not alter the glucose-6-phosphatase activity.
Khan
A
A
Department of Molecular Medicine, Karolinska Hospital, Stockholm, Sweden.
Zong-Chao
L
L
Efendic
S
S
Landau
B R
BR
D000818:Animals; D005952:Glucose-6-Phosphatase / Q000378:metabolism*; D019298:Glucose-6-Phosphate / Q000378:metabolism; D005994:Glycerophosphates / Q000378:metabolism; D006868:Hydrolysis; D007031:Hypothalamus / Q000201:enzymology*; D051379:Mice; D008817:Mice, Mutant Strains / Q000235:genetics; D009765:Obesity / Q000201:enzymology* / Q000235:genetics*; D012016:Reference Values
D005994:Glycerophosphates; D019298:Glucose-6-Phosphate; C029620:alpha-glycerophosphoric acid; D005952:Glucose-6-Phosphatase