B-Cell lymphoma induction by akv murine leukemia viruses harboring one or both copies of the tandem repeat in the U3 enhancer.
Akv is an endogenous, ecotropic murine leukemia virus (MuLV) of the AKR strain. It has served as a prototype nonpathogenic or weakly pathogenic reference virus for studies of closely related potent lymphomagenic viruses such as the T-lymphomagenic SL3-3. We here report that Akv and an Akv mutant (Akv1-99) with only one copy of the 99-bp transcriptional enhancer induce malignant lymphomas with nearly 100% incidence and mean latency periods of 12 months after injection into newborn NMRI mice. Molecular analysis of tumor DNA showed that the majority of the tumors were of the B-cell type. Sequence analysis of proviral transcriptional enhancers in DNA of B-cell lymphomas revealed conservation of the enhancer sequence, as well as a lack of sequence duplications of the Akv1-99 variant, while the repeat copy number in Akv was subject to fluctuations. In support of a B-cell specificity of the Akv enhancer, a murine plasmacytoma cell line was found to sustain three- to fivefold-higher transient transcriptional activity upon the Akv and Akv1-99 enhancers than upon the enhancer of the T-lymphomagenic SL3-3 MuLV. Thus, the overall picture is that Akv MuLV possesses a B- lymphomagenic potential and that the second copy of the 99-bp sequence seems to be of minor importance for this potential. However, in one animal the lymphomas induced by Akv1-99 were of the T-cell type. Among the 24 tumors analyzed only this one harbored a clonal proviral integration in the c-myc locus. This provirus had undergone a duplication of a 113-bp sequence of the enhancer region, partly overlapping with the 99-bp repeat of Akv, as well as a few single nucleotide alterations within and outside the repeats. Taken together with previous studies, our results suggest that T- versus B-lymphomagenic specificity of the enhancer is governed by more than one nucleotide difference and that alterations in binding sites for transcription factors of the AML1 and nuclear-factor-1 families may contribute to this specificity.
Lovmand
J
J
Department of Molecular and Structural Biology, University of Aarhus, DK-8000 Aarhus C, Denmark.
Sorensen
A B
AB
Schmidt
J
J
Ostergaard
M
M
Luz
A
A
Pedersen
F S
FS
D016475:3T3 Cells; D000818:Animals; D004273:DNA, Neoplasm / Q000032:analysis; D004742:Enhancer Elements, Genetic; D015321:Gene Rearrangement; D016259:Genes, myc; D007150:Immunohistochemistry; D009052:Leukemia Virus, Murine / Q000235:genetics* / Q000472:pathogenicity; D016393:Lymphoma, B-Cell / Q000209:etiology*; D051379:Mice; D008806:Mice, Inbred AKR; D017354:Point Mutation; D012091:Repetitive Sequences, Nucleic Acid; D014407:Tumor Cells, Cultured; D016662:Virus Integration
D004273:DNA, Neoplasm