Endotoxin removal with poly(ethyleneimine)-immobilized adsorbers: Sepharose 4B versus flat sheet and hollow fibre membranes.
Poly(ethyleneimine) was immobilized on poly(vinyl alcohol)-coated nylon flat sheet membranes, poly(vinyl alcohol) and poly(ethylenevinyl alcohol) hollow fibre membranes as well as Sepharose 4B. The resulting poly(ethyleneimine)-immobilized adsorbers were used for removal of E. coli derived endotoxin from buffers and bovine serum albumin solutions. The efficiency of poly(ethyleneimine) proved to be constant over a wide pH range, including phosphate buffered saline. The performance depended upon the matrix type employed: endotoxin clearance factors varied from 100 to 120,000 in protein-free solutions and 40 to 33,000 in solutions of bovine serum albumin using 6000 EU/ml as feed concentration. The best adsorber was the flat sheet membrane-immobilized poly(ethyleneimine), followed by the hollow fibre-immobilized poly(ethyleneimine) and poly(ethyleneimine)-Sepharose. The factors influencing endotoxin clearance were the mass transport (convective systems were superior to the diffusive system), the chemical composition and the surface structure of the underlying matrix.
Petsch
D
D
GBF, Gesellschaft für Biotechnologische Forschung mbH, Biochemical Engineering Division, Braunschweig, Germany.
Deckwer
W D
WD
Anspach
F B
FB
Legallais
C
C
Vijayalakshmi
M
M
D000042:Absorption; D002021:Buffers; D004731:Endotoxins / Q000302:isolation & purification*; D004926:Escherichia coli / Q000737:chemistry*; D006863:Hydrogen-Ion Concentration; D008024:Ligands; D008567:Membranes, Artificial; D008628:Mercury; D011094:Polyethyleneimine; D012685:Sepharose; D012710:Serum Albumin, Bovine / Q000737:chemistry; D012996:Solutions
D002021:Buffers; D004731:Endotoxins; D008024:Ligands; D008567:Membranes, Artificial; D012996:Solutions; D012710:Serum Albumin, Bovine; C044101:endotoxin, Escherichia coli; D011094:Polyethyleneimine; D012685:Sepharose; D008628:Mercury