[Characteristics of N-terminal 60-kDa fragment of elongation factor 2].
The N-terminal 60-kDa-fragment of elongation factor 2 from rat liver (EF-2) was obtained by the limited proteolysis of native EF-2 with elastase. This fragment consists of 506 N-terminal amino acid residues of EF-2. The conformational properties of both this fragment and EF-2 in solution were studied by circular dichroism and fluorescent spectroscopy. The contents of secondary structure components in the fragment and in the factor that were deduced from CD measurements agreed well with values predicted from their primary structures. Both proteins were resistant to denaturation with < or = 3 M urea and exhibited cooperative denaturation transitions. Temperature melting also proceeded cooperatively for the fragment and EF-2. Structural properties of the N-terminal 60-kDa-fragment are discussed in comparison with the biochemical characteristics and 3D structure of prokaryotic elongation factor EF-G.
Korotkov
K V
KV
Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Pushchino, Moscow, Russia.
Plotnikov
A N
AN
Motuz
L P
LP
Vasilenko
K S
KS
Semisotnov
G V
GV
Alakhov
Iu B
IuB
D000818:Animals; D002942:Circular Dichroism; D020963:GTP Phosphohydrolase-Linked Elongation Factors / Q000737:chemistry; D008099:Liver / Q000378:metabolism*; D008970:Molecular Weight; D010196:Pancreatic Elastase / Q000737:chemistry; D020652:Peptide Elongation Factor 2; D020653:Peptide Elongation Factor G; D010445:Peptide Elongation Factors / Q000737:chemistry*; D010446:Peptide Fragments / Q000737:chemistry*; D010750:Phosphoproteins / Q000737:chemistry*; D011487:Protein Conformation; D011489:Protein Denaturation; D017433:Protein Structure, Secondary; D017434:Protein Structure, Tertiary; D051381:Rats; D014508:Urea / Q000737:chemistry
D020652:Peptide Elongation Factor 2; D020653:Peptide Elongation Factor G; D010445:Peptide Elongation Factors; D010446:Peptide Fragments; D010750:Phosphoproteins; D014508:Urea; D010196:Pancreatic Elastase; D020963:GTP Phosphohydrolase-Linked Elongation Factors
Svoĭstva N-kontsevogo 60-kDa fragmenta faktora élongatsii 2.