title: IgE responses in the serum and gastric lymph of sheep infected with Teladorsagia circumcincta.
abstract: The IgE response of naive or previously infected sheep to 50,000 infective larvae of Teladorsagia circumcincta was monitored in serum and gastric lymph using a monoclonal antibody generated to recombinant ovine IgE in a dot blot assay. In 4/5 naive sheep, lymph and serum IgE concentrations increased from days 8 and 14 after infection, respectively. In most previously infected sheep, the IgE response to challenge was more rapid, although not necessarily greater than that following a primary infection. IgE concentrations in lymph were some 4-fold higher than in serum indicating that its source was the mucosa or draining nodes.
authors.affiliation: Moredun Research Institute, Edinburgh, Scotland, UK.
authors.forename: J F
authors.initials: JF
authors.lastname: Huntley
authors.forename: H D
authors.initials: HD
authors.lastname: Schallig
authors.forename: F N
authors.initials: FN
authors.lastname: Kooyman
authors.forename: A
authors.initials: A
authors.lastname: MacKellar
authors.forename: J
authors.initials: J
authors.lastname: Millership
authors.forename: W D
authors.initials: WD
authors.lastname: Smith
mesh_terms: D000818:Animals; D000909:Antibodies, Helminth / Q000032:analysis* / Q000097:blood; D000911:Antibodies, Monoclonal; D015153:Blotting, Western; D015151:Immunoblotting; D007073:Immunoglobulin E / Q000032:analysis* / Q000097:blood; D008196:Lymph / Q000276:immunology*; D009349:Nematode Infections / Q000097:blood / Q000276:immunology* / Q000469:parasitology; D011994:Recombinant Proteins / Q000032:analysis / Q000097:blood; D012756:Sheep; D012757:Sheep Diseases / Q000097:blood / Q000276:immunology* / Q000469:parasitology; D013270:Stomach; D013997:Time Factors
chemical_list: D000909:Antibodies, Helminth; D000911:Antibodies, Monoclonal; D011994:Recombinant Proteins; D007073:Immunoglobulin E