Sequence and preliminary characterisation of a Taenia saginata oncosphere gene homologue of the small heat-shock protein family.
During antibody screening of a Taenia saginata oncosphere cDNA library a clone (R-Tso2) sharing a high degree of homology at both the DNA and amino acid levels with the small heat-shock protein (shsp) family was identified. The R-Tso2 clone was a full-length sequence (1162 bp) with an open reading frame of 945 bp and 314 amino acids, corresponding to a deduced molecular mass of 35.6 kDa and isoelectric point of 5.6. R-Tso2 had the highest degree of homology with the Schistosoma mansoni major egg antigens, showing the characteristic shsp 100 amino-acid sequence motif duplicated. The R-Tso2 expression product was not immuno-precipitated by any serum from a panel of serum samples obtained from bovine, porcine and human hosts suffering from either T. saginata or T. solium cysticercosis.
Benitez
L
L
Ministerio de Sanidad y Consumo, Instituto de Salud Carlos III, CNM, Madrid, Spain.
Harrison
L J
LJ
Parkhouse
R M
RM
Garate
T
T
D000595:Amino Acid Sequence; D000818:Animals; D000947:Antigens, Helminth / Q000737:chemistry / Q000235:genetics / Q000276:immunology; D001483:Base Sequence; D003001:Cloning, Molecular; D003551:Cysticercosis / Q000276:immunology / Q000662:veterinary; D018076:DNA, Complementary; D017238:Genes, Helminth; D006360:Heat-Shock Proteins / Q000737:chemistry / Q000235:genetics* / Q000276:immunology / Q000378:metabolism*; D006801:Humans; D008969:Molecular Sequence Data; D011994:Recombinant Proteins / Q000737:chemistry / Q000276:immunology; D012550:Schistosoma mansoni / Q000737:chemistry / Q000235:genetics / Q000276:immunology; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid; D013621:Taenia / Q000737:chemistry* / Q000235:genetics* / Q000276:immunology
D000947:Antigens, Helminth; D018076:DNA, Complementary; D006360:Heat-Shock Proteins; C113104:R-Tso2 protein, Taenia saginata; D011994:Recombinant Proteins