title: Isolation and identification of a second diuretic hormone from Tenebrio molitor.
abstract: A diuretic hormone (DH) of unusual structure was isolated from extracts of heads of Tenebrio molitor. The hormone is a 47 amino acid peptide, Mr = 5,029.9, with the sequence AGALGESGASLSIVNSLDVLRNRLLLEIARKKAKEGANRNRQILLSL. This peptide increases cyclic AMP production in Malpighian tubules of T. molitor. We recently identified a smaller DH from T. molitor with 37 amino acids; these peptides have only 15 identical amino acids when aligned to maximize similarity to other members of the insect DH family. This family has sequence similarity to the corticotropin-releasing factor superfamily of vertebrate peptides.
authors.affiliation: Department of Biochemistry, University of Nevada, Reno 89557, USA.
authors.forename: K
authors.initials: K
authors.lastname: Furuya
authors.forename: K M
authors.initials: KM
authors.lastname: Schegg
authors.forename: D A
authors.initials: DA
authors.lastname: Schooley
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D003346:Corticotropin-Releasing Hormone / Q000737:chemistry; D000242:Cyclic AMP / Q000378:metabolism; D004232:Diuretics / Q000737:chemistry* / Q000494:pharmacology; D006257:Head; D006728:Hormones / Q000737:chemistry* / Q000494:pharmacology; D019476:Insect Proteins / Q000737:chemistry* / Q000494:pharmacology; D008317:Malpighian Tubules / Q000187:drug effects; D008969:Molecular Sequence Data; D005810:Multigene Family; D017386:Sequence Homology, Amino Acid; D013712:Tenebrio / Q000737:chemistry*
chemical_list: D004232:Diuretics; D006728:Hormones; D019476:Insect Proteins; D003346:Corticotropin-Releasing Hormone; D000242:Cyclic AMP