Characterization of anti-feline CD8 monoclonal antibodies.
We generated three monoclonal antibodies (mAbs) (2D7, 10C7 and 12A3) reactive to the alpha-chain of feline CD8 (fCD8) molecule. Further we showed that reference anti-fCD8 mAbs, FT2, 3.357 and vpg9 recognize the beta-chain, alpha-chain and alphabeta-complex epitope, respectively. Flow cytometric analysis using these mAbs suggested that fCD8alpha(+)beta(-) cells were present in lymphocytes of spleen, but not significantly in those of thymus, lymph nodes and peripheral blood of normal kittens.
Shimojima
M
M
Department of Veterinary Microbiology, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Japan.
Pecoraro
M R
MR
Maeda
K
K
Tohya
Y
Y
Miyazawa
T
T
Mikami
T
T
D000818:Animals; D000911:Antibodies, Monoclonal / Q000032:analysis* / Q000096:biosynthesis; D016827:CD8 Antigens / Q000235:genetics / Q000276:immunology*; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology; D019556:COS Cells; D002415:Cats / Q000276:immunology*; D002478:Cells, Cultured; D017931:DNA Primers / Q000737:chemistry; D000939:Epitopes; D005434:Flow Cytometry / Q000662:veterinary; D019084:Fluorescent Antibody Technique, Indirect / Q000662:veterinary; D015870:Gene Expression; D051379:Mice; D008807:Mice, Inbred BALB C; D010957:Plasmids
D000911:Antibodies, Monoclonal; D016827:CD8 Antigens; D017931:DNA Primers; D000939:Epitopes