title: Two-dimensional polyacrylamide gel electrophoresis map of bull seminal plasma proteins.
abstract: A two-dimensional polyacrylamide gel electrophoresis map of bull seminal plasma proteins has been established. About 250 spots were detected after silver staining and polypeptides from 24 spots have been N-terminally sequenced. Major proteins already described in bull seminal plasma, like PDC-109 and aSFP, have been located on the map; proteins not yet reported in male reproductive tracts have been evidenced; for some polypeptides showing a previously unknown N-terminal sequence, structural similarities with proteins described in other organisms have been found. A reference map of seminal plasma proteins could be useful in relating protein pattern changes to physiopathological events influencing the reproductive sphere.
authors.affiliation: Istituto di Fisiologia Veterinaria e Biochimica, Università degli Studi di Milano, Italy.
authors.forename: M
authors.initials: M
authors.lastname: Mortarino
authors.forename: G
authors.initials: G
authors.lastname: Tedeschi
authors.forename: A
authors.initials: A
authors.lastname: Negri
authors.forename: F
authors.initials: F
authors.lastname: Ceciliani
authors.forename: L
authors.initials: L
authors.lastname: Gottardi
authors.forename: G
authors.initials: G
authors.lastname: Maffeo
authors.forename: S
authors.initials: S
authors.lastname: Ronchi
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D002417:Cattle; D015180:Electrophoresis, Gel, Two-Dimensional / Q000379:methods*; D008297:Male; D008969:Molecular Sequence Data; D010449:Peptide Mapping / Q000379:methods*; D029584:Prostatic Secretory Proteins; D011506:Proteins / Q000032:analysis*; D029589:Seminal Plasma Proteins
chemical_list: D029584:Prostatic Secretory Proteins; D011506:Proteins; D029589:Seminal Plasma Proteins; C043076:beta-microseminoprotein