Platelets interact with soluble and insoluble collagens through characteristically different reactions.
Platelet interaction with soluble and insoluble collagens was characterized through binding studies. In contrast to resting platelets, cells reacted with activators, TS2/16 (integrin alpha2 beta1-activating antibody), thrombin, collagen-related peptide, or ADP, exhibited specific soluble collagen binding that is Mg2+-dependent, but inhibited by prostaglandin I2, Ca2+, and Gi9 (anti-integrin alpha2 beta1 antibody). Each platelet has 1500-3500 soluble collagen binding sites, with a dissociation constant of 3. 5-9 x 10(-8) M. This is the first study to show the specific binding of soluble collagen to platelets; our data strongly suggest that the receptor is integrin alpha2 beta1 after it becomes activated upon platelet activation. These results suggest that activation of platelets transforms integrin alpha2 beta1 to a state with higher affinity binding sites for soluble collagen. The soluble collagen-platelet interaction was compared with the platelet interaction with fibrillar collagen, which has until now not been demonstrated to bind specifically to platelets. Here, we demonstrated specific, biphasic fibrillar collagen binding. One phase is rapid and metal ion-independent, and accounts for most of the binding. The other phase is slow and Mg2+-dependent. The characteristic differences in the specific bindings of soluble and fibrous collagens demonstrate the different contributions of two different collagen receptors.
Jung
S M
SM
Department of Protein Biochemistry, Institute of Life Science, Kurume University, Kurume-shi, Fukuoka-ken 839-8016, Japan. smj@ktarn.or.jp
Moroi
M
M
D000818:Animals; D000906:Antibodies / Q000494:pharmacology; D001792:Blood Platelets / Q000502:physiology*; D002118:Calcium / Q000494:pharmacology; D002417:Cattle; D003094:Collagen / Q000378:metabolism*; D011464:Epoprostenol / Q000494:pharmacology; D006801:Humans; D016023:Integrins / Q000276:immunology / Q000378:metabolism / Q000502:physiology; D007700:Kinetics; D008274:Magnesium / Q000494:pharmacology; D008562:Membrane Glycoproteins / Q000276:immunology; D015539:Platelet Activation / Q000187:drug effects; D011485:Protein Binding / Q000187:drug effects; D038981:Receptors, Collagen; D012995:Solubility
D000906:Antibodies; D016023:Integrins; D008562:Membrane Glycoproteins; D038981:Receptors, Collagen; D003094:Collagen; D011464:Epoprostenol; D008274:Magnesium; D002118:Calcium