Development of agents for reinforcement of fluorescence on near-infrared ray excitation for immunohistological staining.
Fluorescence intensity of indocyanine green (ICG) derivative (ICG-sulfo-OSu) was too low for its use to detect microlesions. Therefore, we examined the effects of reinforcement agents on ICG-sulfo-OSu labeled antibodies. Solutions of distearoylphosphatic acid sodium salt (DSPA) and octylglucoside (OG) in physiological phosphate buffered saline (PBS) were found to increase the intensity of fluorescence of ICG-sulfo-OSu labeled antibodies, with shift in the fluorescence peak wavelength from 804 to 821 nm.
Ito
S
S
Second Department of Internal Medicine, School of Medicine, University of Tokushima, Japan. ito@clin.med.tokushima-u.ac.jp
Muguruma
N
N
Hayashi
S
S
Taoka
S
S
Bando
T
T
Inayama
K
K
Sogabe
M
M
Okahisa
T
T
Okamura
S
S
Shibata
H
H
Irimura
T
T
Takesako
K
K
Shibamura
S
S
D000906:Antibodies / Q000737:chemistry; D005455:Fluorescent Antibody Technique; D005456:Fluorescent Dyes / Q000592:standards*; D007208:Indocyanine Green / Q000031:analogs & derivatives* / Q000737:chemistry; D013055:Spectrophotometry, Infrared / Q000379:methods*
D000906:Antibodies; D005456:Fluorescent Dyes; C113792:indocyanine green-sulfo-OSu; D007208:Indocyanine Green