Dimethylsulfoxide-induced cell death of murine erythroleukemia cells exposed to ionising radiation.
The present investigation was aimed at studying the effects of dimethylsulfoxide (DMSO) in combination with high dose (15 and 60 Gy) ionising radiation on the growth and differentiation of murine erythroleukemia cells (MEL). The incubation with DMSO was performed for 96 h starting immediately after exposure to radiation and resulted only in a slight inhibition of cell growth and in a high increase in cell death with the induction of both necrosis and apoptosis. The enhancement of radiation cytotoxicity was directly related to dose, time in culture and degree of differentiation as demonstrated by the severe and multiple aberrations observed in light and electron microscopy. Of interest was the observation in induced cells of a marked rearrangement of the plasma membrane architecture as well as that of the nuclear envelope, with a massive translocation and/or decrease in the nuclear pore complexes.
Di Pietro
R
R
Istituto di Morfologia Umana Normale, Chieti, Italy. r.dipietro@morpho.unich.it
Centurione
M A
MA
Falcieri
E
E
Centurione
L
L
Santavenere
E
E
Rana
R
R
D000818:Animals; D016923:Cell Death / Q000187:drug effects / Q000528:radiation effects; D002454:Cell Differentiation / Q000187:drug effects / Q000528:radiation effects; D002467:Cell Nucleus / Q000648:ultrastructure; D004121:Dimethyl Sulfoxide / Q000494:pharmacology*; D004307:Dose-Response Relationship, Radiation; D004915:Leukemia, Erythroblastic, Acute / Q000473:pathology*; D051379:Mice; D009685:Nuclear Envelope / Q000648:ultrastructure; D011839:Radiation, Ionizing; D014407:Tumor Cells, Cultured
D004121:Dimethyl Sulfoxide