title: Rapid solid-phase extraction method for automated gas chromatographic-mass spectrometric determination of nicotine in plasma.
abstract: The aim of this study was to develop a simple, rapid and sensitive assay of nicotine in plasma for automated gas chromatographic-mass spectrometric analysis. Biological samples were extracted using pre-packed Extrelut-1 columns with 5 ml of ethyl acetate. Quantitative analysis was done using deuterium-labelled nicotine as internal standard. The limit of quantitation was 0.5 ng in 1-ml plasma samples. Precision was ranging from 13.3% to 1.64% (R.S.D.) depending on the concentration, while the deviation was 4.16%. This method has been used for determination of nicotine bioavailability from new, low-dosage, nicotine chewing gum strips.
authors.affiliation: Istituto di Ricerche Farmacologiche Mario Negri, Milan, Italy.
authors.forename: E
authors.initials: E
authors.lastname: Davoli
authors.forename: L
authors.initials: L
authors.lastname: Stramare
authors.forename: R
authors.initials: R
authors.lastname: Fanelli
authors.forename: L
authors.initials: L
authors.lastname: Diomede
authors.forename: M
authors.initials: M
authors.lastname: Salmona
mesh_terms: D000328:Adult; D001322:Autoanalysis; D001682:Biological Availability; D002638:Chewing Gum; D008401:Gas Chromatography-Mass Spectrometry; D006801:Humans; D008297:Male; D009538:Nicotine / Q000008:administration & dosage / Q000097:blood* / Q000493:pharmacokinetics; D018722:Nicotinic Agonists / Q000008:administration & dosage / Q000097:blood* / Q000493:pharmacokinetics; D012015:Reference Standards; D015203:Reproducibility of Results
chemical_list: D002638:Chewing Gum; D018722:Nicotinic Agonists; D009538:Nicotine