title: Nitric oxide synthase induction in lines of macrophages from different anatomical sites.
abstract: Induction of nitric oxide synthase (iNOS) and nitric oxide (NO) production have been demonstrated using three macrophage cell lines from different anatomical sites, which had been immortalized using a rapid and convenient procedure previously described. Lysis of tumor cells presumably was caused by NO accumulation in the supernatants of cultures of the three cell lines after induction with a mixture of recombinant murine interferon gamma (rMuIFNgamma) and lipopolysaccharide (LPS). Induction by these two biological response modifiers (BRM) caused lysis of tumor cells and was repressed by addition of 1 microM (final concentration) of methyl-L-arginine (MMA) to the mixture during induction of the enzyme. Research using readily generated macrophage cell lines may facilitate clarify basic aspects of iNOS induction in these cells.
authors.affiliation: Department of Oncology, Hoffmann-La Roche, Inc., Nutley, New Jersey, USA.
authors.forename: A V
authors.initials: AV
authors.lastname: Palleroni
authors.forename: S
authors.initials: S
authors.lastname: Hajos
authors.forename: R B
authors.initials: RB
authors.lastname: Wright
authors.forename: N J
authors.initials: NJ
authors.lastname: Palleroni
mesh_terms: D000818:Animals; D002460:Cell Line; D004790:Enzyme Induction; D005260:Female; D019085:Fluorescent Antibody Technique, Direct; D008264:Macrophages / Q000187:drug effects / Q000378:metabolism*; D051379:Mice; D008810:Mice, Inbred C57BL; D019001:Nitric Oxide Synthase / Q000096:biosynthesis*; D052247:Nitric Oxide Synthase Type II; D009573:Nitrites / Q000378:metabolism; D019323:omega-N-Methylarginine / Q000494:pharmacology
chemical_list: D009573:Nitrites; D019323:omega-N-Methylarginine; D019001:Nitric Oxide Synthase; D052247:Nitric Oxide Synthase Type II; C496317:Nos2 protein, mouse