Molecular cloning and sequencing of the rat interleukin-12 p40 gene.
The nucleotide sequences containing the rat interleukin-12 p40 gene was determined. Sequencing revealed the presence of six exons and five introns. Analysis of the 5' non-coding region showed the presence of several possible sites involved in cytokine gene regulation at the transcriptional level. Comparison of the deduced amino acid sequence of rat IL-12 p40 with that of the mouse and of human p40, showed 92% and 65% identity respectively.
Khalife
J
J
Centre d'Immunologie et de Biologie Parasitaire, INSERM U. 167, Institut Pasteur, Lille, France.
Pierrot
C
C
Cocude
C
C
Cêtre
C
C
Godin
C
C
Capron
A
A
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D001665:Binding Sites / Q000235:genetics; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000737:chemistry / Q000235:genetics; D005091:Exons / Q000235:genetics; D005786:Gene Expression Regulation; D005796:Genes / Q000235:genetics*; D018664:Interleukin-12 / Q000737:chemistry / Q000235:genetics*; D007438:Introns / Q000235:genetics; D008969:Molecular Sequence Data; D051381:Rats; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D014158:Transcription, Genetic / Q000235:genetics
D018076:DNA, Complementary; D018664:Interleukin-12