Viper venom-induced inflammation and inhibition of free radical formation by pure compound (2-hydroxy-4-methoxy benzoic acid) isolated and purified from anantamul (Hemidesmus indicus R. BR) root extract.
The present investigation explored the possible venom neutralizing effect of a pure compound (2-hydroxy-4-methoxy benzoic acid) isolated and purified from the methanolic root extract of Hemidesmus indicus R.Rr. 2-OH-4-MeO benzoic acid possessed potent anti-inflammatory, antipyretic and antioxidant properties. The compound effectively neutralized inflammation induced by Vipera russelli venom in male albino mice and reduced cotton pellet-induced granuloma in rats. The compound produced a significant fall in body temperature in yeast-induced pyrexia in rats but did not change the normothermic body temperature. The compound effectively neutralized viper venom-induced changes in serum phosphatase and transaminase activity in male albino rats. It also neutralized free radical formation as estimated by TBAPS and superoxide dismutase activities. The antisnake venom activity of the pure compound is partly mediated through the above physiological process.
Alam
M I
MI
Department of Physiology, University of Calcutta, India.
Gomes
A
A
D000135:Acid Phosphatase / Q000037:antagonists & inhibitors / Q000097:blood; D000469:Alkaline Phosphatase / Q000037:antagonists & inhibitors / Q000097:blood; D000818:Animals; D000893:Anti-Inflammatory Agents / Q000494:pharmacology; D001565:Benzoates / Q000737:chemistry / Q000302:isolation & purification*; D001831:Body Temperature / Q000187:drug effects; D005609:Free Radicals; D006168:Guinea Pigs; D007249:Inflammation / Q000139:chemically induced*; D015227:Lipid Peroxidation / Q000187:drug effects; D008297:Male; D008515:Medicine, Ayurvedic; D051379:Mice; D010936:Plant Extracts / Q000737:chemistry*; D018517:Plant Roots / Q000737:chemistry*; D010946:Plants, Medicinal / Q000737:chemistry*; D051381:Rats; D017840:Daboia; D014757:Viper Venoms / Q000494:pharmacology*
C112536:2-hydroxy-4-methoxy benzoic acid; D000893:Anti-Inflammatory Agents; D001565:Benzoates; D005609:Free Radicals; D010936:Plant Extracts; D014757:Viper Venoms; D000469:Alkaline Phosphatase; D000135:Acid Phosphatase