Comparison of the immunological and protective responses elicited by microencapsulated formulations of the F1 antigen from Yersinia pestis.
Purified native F1 antigen from Yersinia pestis was used to assess controlled-release vaccine delivery systems in poly(lactide-co-glycolide) (PLG) microparticles and liposomes. Antigen encapsulated in PLG microparticles induced high serum titres when injected i.p. in mice: mucosal IgA was also detected. Mice immunized with F1 in Alhydrogel or PLGs were protected against subcutaneous challenge with Y. pestis. F1 antigen surface-labelled onto liposome vesicles stimulated high serum titres in Balb/c mice and also induced a mucosal response: F1-labelled liposomes protected mice against challenge with up to 1 x 10(5) organisms. These findings indicate that a significant immune response is induced by immunizing with F1 formulated in PLGs and liposomes and that protection was achieved after only one dose.
Reddin
K M
KM
Centre for Applied Microbiology and Research (CAMR), Salisbury, Wiltshire, UK.
Easterbrook
T J
TJ
Eley
S M
SM
Russell
P
P
Mobsby
V A
VA
Jones
D H
DH
Farrar
G H
GH
Williamson
E D
ED
Robinson
A
A
D000284:Administration, Oral; D000536:Aluminum Hydroxide; D000818:Animals; D000907:Antibodies, Bacterial / Q000096:biosynthesis; D001426:Bacterial Proteins / Q000008:administration & dosage / Q000276:immunology*; D001428:Bacterial Vaccines / Q000276:immunology*; D004339:Drug Compounding; D005069:Evaluation Studies as Topic; D005260:Female; D007070:Immunoglobulin A / Q000096:biosynthesis; D007273:Injections, Intramuscular; D007274:Injections, Intraperitoneal; D008081:Liposomes; D051379:Mice; D008807:Mice, Inbred BALB C; D011098:Polyglactin 910; D015009:Yersinia Infections / Q000276:immunology / Q000517:prevention & control*; D015010:Yersinia pestis / Q000276:immunology*
D000907:Antibodies, Bacterial; D001426:Bacterial Proteins; D001428:Bacterial Vaccines; D007070:Immunoglobulin A; D008081:Liposomes; C086725:caf1 protein, Yersinia pestis; D011098:Polyglactin 910; D000536:Aluminum Hydroxide