Use of simulated blood cultures to compare a specific fungal medium with a standard microorganism medium for yeast detection.
The aim of this study was to compare the performance of a specific blood culture medium recently developed to detect fungi on the Bactec 9240 system (Mycosis IC/F; Becton Dickinson Diagnostic Instrument Systems, USA) with that of the standard medium used for detection of bacteria (Aerobic Plus/F; Becton Dickinson). Simulated blood cultures were performed on 43 strains belonging to ten fungal species using the two media. The mean time to yeast detection using the Mycosis IC/F medium was 29.03+/-13.99 h, in contrast to a mean time of 73.92+/-56.74 h using the Aerobic Plus/F medium. These results show that the Mycosis IC/F medium clearly reduces the time to yeast detection on the Bactec 9240.
Fricker-Hidalgo
H
H
Département de Parasitologie-Mycologie Médicale et Moléculaire, Centre Hospitalier Universitaire, Grenoble, France.
Chazot
F
F
Lebeau
B
B
Pelloux
H
H
Ambroise-Thomas
P
P
Grillot
R
R
D001331:Automation; D001769:Blood / Q000382:microbiology; D003470:Culture Media; D016469:Fungemia / Q000175:diagnosis*; D006801:Humans; D009172:Mycology / Q000379:methods; D011933:Reagent Kits, Diagnostic; D015003:Yeasts / Q000254:growth & development* / Q000302:isolation & purification*
D003470:Culture Media; D011933:Reagent Kits, Diagnostic