Bioluminescence of free and poly(vinyl alcohol) cryogel-entrapped recombinant Escherichia coli cells expressing firefly luciferase.
Bioluminescence of free and poly(vinyl alcohol) cryogel-entrapped recombinant E. coli cells expressing firefly luciferase was investigated. It was shown that bioluminescence intensity and time-course of the bioluminescent signal changed upon immobilization and depended on intracellular ATP concentration and permeability of the cell membrane.
Romanova
N A
NA
School of Chemistry, Lomonosov Moscow State University, Moscow, 119899, Russia. UNN@enzyme.chem.msu.su
Brovko
L Y
LY
Makhlis
T A
TA
Sinodova
E M
EM
Ugarova
N N
NN
D000255:Adenosine Triphosphate / Q000378:metabolism; D000818:Animals; D002463:Cell Membrane Permeability; D001517:Coleoptera / Q000201:enzymology / Q000235:genetics; D004926:Escherichia coli / Q000201:enzymology* / Q000235:genetics*; D017344:Genes, Insect; D007700:Kinetics; D008156:Luciferases / Q000235:genetics* / Q000378:metabolism*; D008163:Luminescent Measurements; D011142:Polyvinyl Alcohol; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism; D011995:Recombination, Genetic
D011994:Recombinant Proteins; D000255:Adenosine Triphosphate; D011142:Polyvinyl Alcohol; D008156:Luciferases