Mirror-image RNA that binds D-adenosine.
A 58-mer L-RNA ligand that binds to naturally occurring D-adenosine with a dissociation constant of 1.7 microM in solution was identified from a combinatorial library employing mirror-design. The corresponding D-RNA ligand shows identical binding affinity to L-adenosine. Reciprocal chiral specificity was also evident from ligand discrimination; the binding affinity of the L-RNA ligand for D-adenosine was 9000-fold greater than its affinity for L-adenosine and vice versa. While the D-RNA ligand was rapidly degraded in human serum, the L-RNA ligand displayed an extraordinary stability. This indicates the potential application of specifically designed L-RNA ligands as stable monoclonal antibody analogues and the development of highly stable L-ribozymes.
Klussmann
S
S
Institut für Biochemie, Freie Universität Berlin, Germany.
Nolte
A
A
Bald
R
R
Erdmann
V A
VA
Fürste
J P
JP
D000241:Adenosine / Q000737:chemistry / Q000378:metabolism*; D001483:Base Sequence; D001665:Binding Sites; D001667:Binding, Competitive; D001709:Biotechnology; D016384:Consensus Sequence; D017124:Conserved Sequence; D004247:DNA / Q000235:genetics; D004355:Drug Stability; D006801:Humans; D066298:In Vitro Techniques; D008024:Ligands; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation; D012313:RNA / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*; D012689:Sequence Homology, Nucleic Acid; D013237:Stereoisomerism
D008024:Ligands; D012313:RNA; D004247:DNA; D000241:Adenosine