title: Recombination signature of germline immunoglobulin variable genes.
abstract: In human and mouse, the germline contains a tandem array of highly homologous variable (V) gene elements which encode part of the antigen-binding region of the antibody protein. During evolution this array apparently arose by gene duplication followed by diversification of duplicated genes via point mutation and recombination. Analysis of germline V gene sequences using a novel algorithm shows that major recombination sites coincide with the borders of the leader intron and the cap site, consistent with the hypothesis that over evolutionary time cDNA derived by reverse transcription of pre-mRNA in B lymphocytes has recombined with germline DNA.
authors.affiliation: Bioinformatics Laboratory, Research School of Biological Sciences, Australian National University, Canberra, Australian Capital Territory.
authors.forename: G F
authors.initials: GF
authors.lastname: Weiller
authors.forename: H S
authors.initials: HS
authors.lastname: Rothenfluh
authors.forename: P
authors.initials: P
authors.lastname: Zylstra
authors.forename: L M
authors.initials: LM
authors.lastname: Gay
authors.forename: H
authors.initials: H
authors.lastname: Averdunk
authors.forename: E J
authors.initials: EJ
authors.lastname: Steele
authors.forename: R V
authors.initials: RV
authors.lastname: Blanden
mesh_terms: D000465:Algorithms; D000818:Animals; D001483:Base Sequence; D005260:Female; D005803:Genes, Immunoglobulin / Q000235:genetics*; D007135:Immunoglobulin Variable Region / Q000235:genetics*; D007438:Introns / Q000235:genetics; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D008969:Molecular Sequence Data; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D011995:Recombination, Genetic; D016415:Sequence Alignment; D014158:Transcription, Genetic / Q000235:genetics
chemical_list: D007135:Immunoglobulin Variable Region