title: Covalent complex of phenylalanyl-tRNA synthetase with 4-thiouridine-substituted tRNA(Phe) gene transcript retains aminoacylation activity.
abstract: s4U-containing transcripts of tRNA(Phe) gene have been prepared by complete substitution of 16 U residues or by random incorporation of s4U residues followed by affinity electrophoresis isolation of s4U-monosubstituted tRNA transcripts. Both analogs have been cross-linked to Thermus thermophilus phenylalanyl-tRNA synthetase (PheRS) and the specificity of the cross-linking has been demonstrated. Functional activity of the covalent complex of PheRS with the s4U-monosubstituted transcript has been shown by aminoacylation of 60% of the enzyme-cross-linked tRNA. This is the first instance in which biological activity of aminoacyl-tRNA synthetase and cross-linked tRNA in a specific complex has been revealed.
authors.affiliation: Novosibirsk Institute of Bioorganic Chemistry, Siberian Division of the Russian Academy of Sciences.
authors.forename: N A
authors.initials: NA
authors.lastname: Moor
authors.forename: A
authors.initials: A
authors.lastname: Favre
authors.forename: O I
authors.initials: OI
authors.lastname: Lavrik
mesh_terms: D000215:Acylation; D009690:Nucleic Acid Conformation; D010652:Phenylalanine-tRNA Ligase / Q000378:metabolism*; D012360:RNA, Transfer, Phe / Q000737:chemistry / Q000378:metabolism*; D016963:Thermus thermophilus / Q000378:metabolism; D013891:Thiouridine / Q000737:chemistry
chemical_list: D012360:RNA, Transfer, Phe; D013891:Thiouridine; D010652:Phenylalanine-tRNA Ligase