Preparation of heat-labile antigens on air-dried cytologic specimens for immunocytochemical analysis.
OBJECTIVE
To demonstrate the efficacy of Zamboni's solution and storage medium for immunocytochemistry of Ki-67, p53 tumor suppressor gene product and CD3 on long-preserved, air-dried cytologic specimens.


METHODS
Air-dried imprint cytologic specimens obtained from 13 lymphatic tissues and 13 colorectal cancers were fixed with Zamboni's solution and stored for various periods in storage medium including 50% glycerol at -10 to -20 degree C. Mouse monoclonal antibody PC, PAb 1801 and anti-Leu-4 were used for immunostaining.


RESULTS
Immunocytochemical identification of Ki-67, p53 and CD3 was possible on specimens stored for 36, 32 and 16 weeks, respectively.


CONCLUSIONS
Our method, using Zamboni's solution and storage medium, is feasible for immunocytochemistry on long-preserved, air-dried cytologic specimens using the three antibodies were employed.
Baba
N
N
2nd Department of Surgery, Faculty of Medicine, University of Tokyo, Japan.
Kawabata
H
H
Kanno
T
T
Ueno
T
T
Makuuchi
M
M
Morishita
Y
Y
Mori
S
S
Sasaki
J
J
Soejima
K
K
D000388:Air; D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D000951:Antigens, Neoplasm / Q000032:analysis* / Q000276:immunology; D014408:Biomarkers, Tumor / Q000032:analysis* / Q000276:immunology; D017252:CD3 Complex / Q000032:analysis* / Q000276:immunology; D015179:Colorectal Neoplasms / Q000737:chemistry* / Q000473:pathology; D003890:Desiccation; D005404:Fixatives; D006358:Hot Temperature; D006801:Humans; D007124:Immunoenzyme Techniques; D019394:Ki-67 Antigen / Q000032:analysis* / Q000276:immunology; D008198:Lymph Nodes / Q000737:chemistry / Q000473:pathology; D008221:Lymphoid Tissue / Q000737:chemistry* / Q000473:pathology; D051379:Mice; D014066:Palatine Tonsil / Q000737:chemistry / Q000473:pathology; D011309:Preservation, Biological / Q000379:methods*; D011489:Protein Denaturation; D013048:Specimen Handling / Q000379:methods*; D013997:Time Factors; D016159:Tumor Suppressor Protein p53 / Q000032:analysis* / Q000276:immunology
D000911:Antibodies, Monoclonal; D000951:Antigens, Neoplasm; D014408:Biomarkers, Tumor; D017252:CD3 Complex; D005404:Fixatives; D019394:Ki-67 Antigen; D016159:Tumor Suppressor Protein p53