Calcium transport by Frankia sp. strain EAN1pec.
When incubated at 25 degrees C, N2-grown cells of Frankia strain EAN1pec actively accumulated calcium, while NH4Cl-grown cells did not accumulate calcium. When incubated at 0 degrees C, both N2-grown and NH4Cl-grown cells did not actively accumulate calcium. Inhibitors of respiration inhibited calcium accumulation by N2-grown cells at 25 degrees C. Isolated vesicles also accumulated calcium in an energy- and temperature-dependent manner. Two lines of evidence show that Frankia strain EAN1pec has an active calcium extrusion mechanism. First, NH4Cl-grown cells incubated under deenergizing conditions accumulated calcium. Second, calcium efflux from calcium-loaded cells required an energy source and was blocked by inhibitors. The results of this study indicate that Frankia strain EAN1pec has two systems for calcium transport: a calcium extrusion system and a developmentally regulated calcium uptake system.
Tisa
L S
LS
Department of Microbiology, University of New Hampshire, Durham, NH 03824-2617, USA.
D000192:Actinomycetales / Q000378:metabolism*; D000643:Ammonium Chloride / Q000378:metabolism; D002118:Calcium / Q000378:metabolism*; D002132:Calcium Radioisotopes / Q000378:metabolism; D013696:Temperature; D013997:Time Factors
D002132:Calcium Radioisotopes; D000643:Ammonium Chloride; D002118:Calcium