title: Physical and linkage mapping of the bovine bone morphogenetic protein 1 on the evolutionary break region of BTA 8.
abstract: Bone morphogenetic protein 1 (BMP1) has been proposed as a regulatory molecule involved in the binding or activation of other BMP molecules. It has been described as being identical to the enzyme C-proteinase that processes procollagens to fibrillar collagens. A fragment containing an exon of the bovine BMP1 gene was amplified from an adapter-ligated genomic DNA library. The isolated bovine BMP1 fragment was assigned by fluorescence in situ hybridization (FISH) to bovine (BTA) chromosome 8q21, a region containing an evolutionary breakpoint between homology to human (HSA) chromosomes 8 and 9. The assignment to BTA 8 was confirmed by using a hybrid somatic cell panel. The 3' intron region of the amplified genomic BMP1 fragment showed a SSCP polymorphism, and linkage analysis in the International Bovine Reference Panel (IBRP) of families confirmed the boundary position of this gene. The BMP1 gene presents total linkage (theta = 0.00, Z = 3.61) with the lipoprotein lipase gene on BTA 8 and HSA 8 and a larger recombination fraction (theta = 0.11, Z = 5.03) with the marker GGTB2 on BTA 8 and HSA 9. Physical and genetic mapping of the bovine BMP1 gene contribute to narrow the boundary between the HSA 8 and HSA 9 homology segments on BTA 8 and to the comparative mapping between BTA 8, murine chromosome MMU 14, and HSA 8.
authors.affiliation: Laboratorio de Genética Bioquímica, Facultad de Veterinaria, Universidad de Zaragoza, Spain. minma@posta.unizar.es
authors.forename: I
authors.initials: I
authors.lastname: Martín-Burriel
authors.forename: T
authors.initials: T
authors.lastname: Goldammer
authors.forename: C
authors.initials: C
authors.lastname: Elduque
authors.forename: M
authors.initials: M
authors.lastname: Lundin
authors.forename: W
authors.initials: W
authors.lastname: Barendse
authors.forename: P
authors.initials: P
authors.lastname: Zaragoza
authors.forename: I
authors.initials: I
authors.lastname: Olsaker
mesh_terms: D000818:Animals; D001483:Base Sequence; D055395:Bone Morphogenetic Protein 1; D019485:Bone Morphogenetic Proteins / Q000235:genetics*; D002417:Cattle; D002874:Chromosome Mapping; D019143:Evolution, Molecular; D017404:In Situ Hybridization, Fluorescence; D008666:Metalloendopeptidases / Q000235:genetics*; D008969:Molecular Sequence Data
chemical_list: D019485:Bone Morphogenetic Proteins; D008666:Metalloendopeptidases; C525705:BMP1 protein, human; D055395:Bone Morphogenetic Protein 1