[The detection of melanoma metastases in sentinel nodes by polymerase chain reaction].
OBJECTIVE
To investigate the possibility of detecting melanoma metastases using molecular biological techniques in patients with a primary malignant melanoma.


METHODS
Descriptive.


METHODS
Reinier de Graaf Gasthuis Hospital Delft and diagnostic centre SSDZ Delft, the Netherlands.


METHODS
A melanoma specific tyrosinase mRNA was amplified using the reverse transcriptase polymerase chain reaction (RT-PCR) technique.


RESULTS
A total of 23 sentinel nodes derived from 9 patients were examined. In 14 sentinel nodes metastases were found using RT-PCR. In microscopic slides stained with haematoxylin and eosin (HE) melanoma metastases were found in 2 sentinel nodes of 2 patients. With immunohistochemistry melanoma metastases were found in the same 2 sentinel nodes.


CONCLUSIONS
It is possible to detect melanoma metastases in sentinel nodes using molecular biological techniques.
Poelman
M
M
Afd. Chirurgie, Reinier de Graaf Gasthuis, Delft.
van der Velde-Zimmermann
D
D
van Doorn
L J
LJ
Quint
W G
WG
van Krimpen
C
C
de Graaf
P W
PW
D000328:Adult; D000368:Aged; D001706:Biopsy; D005260:Female; D006801:Humans; D007150:Immunohistochemistry / Q000379:methods; D008198:Lymph Nodes / Q000473:pathology*; D008207:Lymphatic Metastasis; D008297:Male; D008545:Melanoma / Q000473:pathology* / Q000556:secondary*; D008875:Middle Aged; D014442:Monophenol Monooxygenase / Q000235:genetics; D016133:Polymerase Chain Reaction / Q000379:methods*; D012333:RNA, Messenger / Q000032:analysis; D012334:RNA, Neoplasm / Q000032:analysis; D012878:Skin Neoplasms / Q000473:pathology*
D012333:RNA, Messenger; D012334:RNA, Neoplasm; D014442:Monophenol Monooxygenase
Opsporing van metastasen van melanomen in schildwachtklieren met de polymerasekettingreactie.