Smooth muscle actin and myosin expression in cultured airway smooth muscle cells.
In this study, the expression of smooth muscle actin and myosin was examined in cultures of rat tracheal smooth muscle cells. Protein and mRNA analyses demonstrated that these cells express alpha- and gamma-smooth muscle actin and smooth muscle myosin and nonmuscle myosin-B heavy chains. The expression of the smooth muscle specific actin and myosin isoforms was regulated in the same direction when growth conditions were changed. Thus, at confluency in 1 or 10% serum-containing medium as well as for low-density cells (50-60% confluent) deprived of serum, the expression of the smooth muscle forms of actin and myosin was relatively high. Conversely, in rapidly proliferating cultures at low density in 10% serum, smooth muscle contractile protein expression was low. The expression of nonmuscle myosin-B mRNA and protein was more stable and was upregulated only to a small degree in growing cells. Our results provide new insight into the molecular basis of differentiation and contractile function in airway smooth muscle cells.
Wong
J Z
JZ
Department of Molecular Physiology, University of Vermont, Burlington 05405, USA.
Woodcock-Mitchell
J
J
Mitchell
J
J
Rippetoe
P
P
White
S
S
Absher
M
M
Baldor
L
L
Evans
J
J
McHugh
K M
KM
Low
R B
RB
D000199:Actins / Q000235:genetics / Q000378:metabolism*; D000818:Animals; D015152:Blotting, Northern; D002478:Cells, Cultured; D003285:Contractile Proteins / Q000378:metabolism; D003598:Cytoskeletal Proteins / Q000378:metabolism; D004591:Electrophoresis, Polyacrylamide Gel; D007150:Immunohistochemistry; D008297:Male; D009130:Muscle, Smooth / Q000166:cytology / Q000378:metabolism*; D009218:Myosins / Q000235:genetics / Q000378:metabolism*; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D011916:Rats, Inbred F344; D014132:Trachea / Q000166:cytology / Q000378:metabolism*
D000199:Actins; D003285:Contractile Proteins; D003598:Cytoskeletal Proteins; D012333:RNA, Messenger; D009218:Myosins