title: Differential adhesion molecule expression on porcine mononuclear cell populations.
abstract: Adhesion molecule expression was analysed on porcine blood and lymphoid organ CD4+ CD8 naive T helper (Th) lymphocytes, CD4+CD8+ memory Th lymphocytes (particular to the pig), CD4-CD8high cytotoxic T (Tc) lymphocytes, CD4 CD8low NK cells (CD3- in the pig), CD4-CD8- T-cell receptor-gammadelta-positive (TCRgammadelta+) lymphocytes, B lymphocytes and monocytes. While CD44 expression was relatively homogeneous amongst mononuclear cells, differences were noted for the integrins. Blood naive Th lymphocytes were CD49d(low)CD11a(low), as were splenic naive Th cells; blood memory Th lymphocytes were CD49d(high)CD11a(low), splenic memory Th cells were CD49d(high)CD11a(high) with a CD49d(high)CD11a(low) subpopulation; blood Tc lymphocytes were mainly CD49d(low)CD11a(low), and splenic cells were CD49d(high) CD11a(high). Lymph node lymphocytes were more homogeneous in their integrin expression. These were relatively CD49d(low)CD11a(low), except the memory Th lymphocytes which had higher integrin expression. B lymphocytes related to the majority of integrin(low) T cells, while monocytes and NK cells were CD49d(high) CD11a(high); gammadelta T lymphocytes showed variable CD49d expression but a CD11a(high) phenotype. CD49d(high) CD11a(high) co-expression was found, and this phenotype was typical of, but not exclusive to, CD25+ (activated) lymphocytes. These results demonstrated that porcine memory Th lymphocytes and NK cells, as well as activated cells, would have increased integrin-dependent activities compared with naive Th lymphocytes, and integrin-dependent reactions would probably vary between blood and lymphoid organ cells.
authors.affiliation: Institute of Virology and Immunoprophylaxis, Mittelhäusern, Switzerland.
authors.forename: M A
authors.initials: MA
authors.lastname: Arriëns
authors.forename: A
authors.initials: A
authors.lastname: Summerfield
authors.forename: K C
authors.initials: KC
authors.lastname: McCullough
mesh_terms: D000818:Animals; D000941:Antigens / Q000276:immunology; D018821:CD18 Antigens / Q000096:biosynthesis; D015815:Cell Adhesion Molecules / Q000096:biosynthesis*; D018960:Hyaluronan Receptors / Q000096:biosynthesis; D019012:Integrin beta1 / Q000096:biosynthesis; D016023:Integrins / Q000096:biosynthesis; D007694:Killer Cells, Natural / Q000166:cytology / Q000378:metabolism; D007963:Leukocytes, Mononuclear / Q000378:metabolism*; D016169:Lymphocyte Function-Associated Antigen-1 / Q000096:biosynthesis; D013552:Swine; D016176:T-Lymphocyte Subsets / Q000166:cytology / Q000378:metabolism; D006377:T-Lymphocytes, Helper-Inducer / Q000276:immunology
chemical_list: D000941:Antigens; D018821:CD18 Antigens; D015815:Cell Adhesion Molecules; D018960:Hyaluronan Receptors; D019012:Integrin beta1; D016023:Integrins; D016169:Lymphocyte Function-Associated Antigen-1