Identification of a UPTG inhibitor protein from maize endosperm: high homology with sucrose synthase protein.
A thermolabile UPTG inhibitor protein (IP) was isolated and purified from a developing maize endosperm preparation. High homology of two internal peptides of IP with known plant sucrose synthase (SS) sequences suggested that IP might be related somehow with SS. IP and SS activities were found in the same preparation and showed thermolability between 60-65 degrees C. IP and SS activities presented the same ionic charge and molecular mass in native conditions (Mono Q and Superose-12 columns chromatographies). Western blot experiments with an anti-SS antibody as well as with an anti-IP antibody showed a single 80 kDa polypeptide band where IP and SS activities were present. Anti-SS antibody can neutralize SS as well as IP activities in a neutralization assay. It was found that in the maize mutant shrunken-1, lacking SS1 protein, the UPTG activity was not inhibited. Furthermore, the solubilized preparation of the sh1 endosperm is unable of inhibiting UPTG activity from potato tuber. The high correlation between IP and SS properties suggests that IP might be in fact a form of SS. Moreover, the relation between IP and the SS1 isoform is discussed. So, a new biological activity of SS is suggested.
Wald
F A
FA
Instituto de Investigaciones Bioquímicas Fundacíon Campomar, Facultad de Ciencias Exactas y Naturales, University of Buenos Aires and CONICET, Argentina.
Rothschild
A
A
Moreno
S
S
Tandecarz
J S
JS
D000595:Amino Acid Sequence; D004791:Enzyme Inhibitors / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D005964:Glucosyltransferases / Q000037:antagonists & inhibitors* / Q000737:chemistry / Q000302:isolation & purification*; D008969:Molecular Sequence Data; D009500:Neutralization Tests; D010940:Plant Proteins / Q000737:chemistry / Q000302:isolation & purification*; D017386:Sequence Homology, Amino Acid; D003313:Zea mays / Q000737:chemistry*
D004791:Enzyme Inhibitors; D010940:Plant Proteins; D005964:Glucosyltransferases; C022699:sucrose synthase; C049577:alpha-1,4-glucan-protein synthase (UDP-forming)