Electrical amygdala kindling in alcohol-withdrawal kindled rats.
Repeated alcohol withdrawal has been shown to kindle seizure activity. The purpose of the present investigation was to study electrical amygdala kindling in rats previously exposed to alcohol-withdrawal kindling. In three independent experiments, male Wistar rats were subjected to multiple episodes each consisting of 2 days of severe alcohol intoxication and 5 days of alcohol withdrawal. In the first experiment, the alcohol-withdrawal kindled animals were divided into two groups depending on whether spontaneous alcohol-withdrawal seizures were observed in episodes 10-13. In the second and third experiments, the alcohol-withdrawal kindled animals were compared to a group in which alcohol-withdrawal kindling was prevented by diazepam treatment during the withdrawal reactions in order to discriminate between the effect of withdrawal and intoxication. Electrical kindling was initiated 28-35 days after the last alcohol dose by exposing the animals to daily electrical stimulations of the right amygdala. The results showed that amygdala kindling was facilitated in alcohol-withdrawal kindled animals which showed spontaneous withdrawal seizure activity, compared with animals exposed to multiple episodes of alcohol withdrawal which did not develop withdrawal seizures or with animals exposed to a single episode of alcohol intoxication. When compared to the control group, the alcohol-withdrawal kindled group with seizures also kindled at a faster rate, but the difference did not reach statistical significance and therefore the results must be regarded as preliminary at present.
Ulrichsen
J
J
Department of Psychiatry O-6234, Rigshospitalet, Copenhagen, Denmark.
Woldbye
D P
DP
Madsen
T M
TM
Clemmesen
L
L
Haugbøl
S
S
Olsen
C H
CH
Laursen
H
H
Bolwig
T G
TG
Hemmingsen
R
R
D000430:Alcohol Withdrawal Delirium / Q000503:physiopathology*; D000435:Alcoholic Intoxication / Q000503:physiopathology; D000679:Amygdala / Q000503:physiopathology*; D000818:Animals; D014151:Anti-Anxiety Agents / Q000494:pharmacology; D003975:Diazepam / Q000494:pharmacology; D004292:Dominance, Cerebral / Q000502:physiology; D004305:Dose-Response Relationship, Drug; D004569:Electroencephalography / Q000187:drug effects; D007696:Kindling, Neurologic / Q000187:drug effects / Q000502:physiology*; D008297:Male; D051381:Rats; D017208:Rats, Wistar
D014151:Anti-Anxiety Agents; D003975:Diazepam