title: The influence of maternal immunity on the development of the in vitro lymphocyte proliferation response against pseudorabies virus in pigs.
abstract: In this study, the influence of maternal immunity against pseudorabies virus (PRV) on the development of a PRV-specific T-cell response in pigs was investigated. Pigs with or without maternal immunity were challenged by inoculation with wild-type PRV, or were vaccinated with 783 and subsequently inoculated. Peripheral blood lymphocytes, collected after vaccination and/or inoculation, were used for PRV-induced lymphocyte proliferation assays in vitro as a measure of a PRV-specific T-cell response. In unvaccinated pigs, the presence of maternal immunity did not inhibit the development of a T-cell response after inoculation with PRV. In contrast, maternal immunity did inhibit the development of a PRV-induced response after intramuscular vaccination. Moreover, vaccinated pigs without maternal immunity shed virus for a shorter period after inoculation than vaccinated pigs with maternal immunity. The greater T-cell response induced by the vaccine might have contributed to the more rapid clearance of PRV in these pigs.
authors.affiliation: DLO-Institute of Animal Science and Health (ID-DLO), Lelystad, The Netherlands. A.Bouma@ID.DLO.NL
authors.forename: A
authors.initials: A
authors.lastname: Bouma
authors.forename: M D
authors.initials: MD
authors.lastname: De Jong
authors.forename: T G
authors.initials: TG
authors.lastname: Kimman
mesh_terms: D000818:Animals; D005260:Female; D011558:Herpesvirus 1, Suid / Q000276:immunology*; D007112:Immunity, Maternally-Acquired; D008213:Lymphocyte Activation; D008214:Lymphocytes / Q000276:immunology / Q000821:virology; D011247:Pregnancy; D011557:Pseudorabies / Q000276:immunology* / Q000517:prevention & control; D022421:Pseudorabies Vaccines; D013552:Swine; D015164:Vaccines, Inactivated; D014765:Viral Vaccines
chemical_list: D022421:Pseudorabies Vaccines; D015164:Vaccines, Inactivated; D014765:Viral Vaccines