Beta-adrenergic receptor number in human lymphocytes is inversely correlated with aerobic capacity.
In the present study, the relationships between beta-adrenergic receptor (beta-AR) expression and aerobic capacity evaluated by maximal oxygen consumption (.VO2max) and oxygen consumption level at ventilatory threshold (.VO2@VT) were investigated. Seventeen physically untrained and 25 trained men participated in the study. After supine resting, the peripheral blood was sampled for preparation of lymphocytes, the model cell used to analyze the beta-AR state. The total number of beta-AR in lymphocytes (beta-ARtotal) was inversely correlated with the VO2 max (r = -0. 368; P < 0.05) and the VO2@VT (r = -0.359; P < 0.05). Similar relationships were also observed between the number of beta-AR in cell surface and both VO2 max (r = -0.491; P < 0.05) and VO2@VT (r = -0.498; P < 0.05). However, no correlation was obtained between the number of beta-AR in intracellular compartments and either VO2 max or VO2@VT. The beta2-AR mRNA level quantified by the use of competitive reverse transcription-polymerase chain reaction was inversely correlated with VO2@VT (r = -0.567; P < 0.05) and positively correlated with beta-ARtotal (r = 0.521; P < 0.05). These findings suggest that the beta-AR number in lymphocytes is inversely correlated with aerobic capacity. This relationship may be explained by downregulation of beta-AR, including internalization with subsequent degradation of the receptors and inhibition of the beta-AR biosynthesis.
Fujii
N
N
Gene Experiment Center, Institute of Applied Biochemistry, University of Tsukuba, Tsukuba-City 305, Japan.
Homma
S
S
Yamazaki
F
F
Sone
R
R
Shibata
T
T
Ikegami
H
H
Murakami
K
K
Miyazaki
H
H
D000293:Adolescent; D000328:Adult; D000332:Aerobiosis; D004837:Epinephrine / Q000097:blood; D006801:Humans; D008214:Lymphocytes / Q000737:chemistry*; D008297:Male; D009638:Norepinephrine / Q000097:blood; D010101:Oxygen Consumption; D010807:Physical Endurance / Q000502:physiology*; D016133:Polymerase Chain Reaction; D012123:Pulmonary Ventilation; D012333:RNA, Messenger / Q000032:analysis; D012194:RNA-Directed DNA Polymerase; D011943:Receptors, Adrenergic, beta / Q000032:analysis* / Q000235:genetics
D012333:RNA, Messenger; D011943:Receptors, Adrenergic, beta; D012194:RNA-Directed DNA Polymerase; D009638:Norepinephrine; D004837:Epinephrine