[The use of proteases for enhancing the sensitivity of detecting ABO system antigens by an absorption-elution reaction in blood stains].
The sensitivity of the absorption-elution test (AET) for the detection of A, B, and H antigens in blood traces can be appreciably increased by using serologically active proteases at different stages of the test. A sensitive AET modification is developed, detecting group antigens in far lesser amounts of the material than by routine AET without proteases. A higher sensitivity of the antigen-antibody reaction between enzyme-treated erythrocytes and tested blood traces permits the use of standard group-specific sera less active than usually; the actually solves the problem of deficit of the sera fit for this test.
Sakharov
R S
RS
Fedulova
M V
MV
Gureeva
N B
NB
Nofal'
Kh K
KhK
Druz'
A F
AF
Shupik
Iu P
IuP
D000017:ABO Blood-Group System / Q000097:blood*; D000042:Absorption; D000910:Antibodies, Heterophile / Q000097:blood; D000911:Antibodies, Monoclonal / Q000097:blood; D001801:Blood Stains; D005554:Forensic Medicine / Q000379:methods; D006388:Hemagglutinins / Q000097:blood; D006801:Humans; D007202:Indicators and Reagents; D010206:Papain; D012680:Sensitivity and Specificity; D012697:Serine Endopeptidases; D012996:Solutions
D000017:ABO Blood-Group System; D000910:Antibodies, Heterophile; D000911:Antibodies, Monoclonal; D006388:Hemagglutinins; D007202:Indicators and Reagents; D012996:Solutions; D012697:Serine Endopeptidases; C088701:protease C; D010206:Papain
Primenenie proteaz dlia povysheniia chuvstvitel'nosti vyiavleniia antigenov sistemy ABO reaktsieĭ absorptsii-éliutsii v sledakh krovi.