title: In vitro hydrogen production by glucose dehydrogenase and hydrogenase.
abstract: A new in vitro enzymatic pathway for the generation of molecular hydrogen from glucose has been demonstrated. The reaction is based on the oxidation of glucose by Thermoplasma acidophilum glucose dehydrogenase with the concomitant oxidation of NADPH by Pyrococcus furiosus hydrogenase. Stoichiometric yields of hydrogen were produced from glucose with the continuous recycling of cofactor. This simple system may provide a method for the biological production of hydrogen from renewable sources. In addition, the other product of this reaction, gluconic acid, is a high-value chemical commodity.
authors.affiliation: Chemical Technology Division, Oak Ridge National Laboratory, TN 37831-6194, USA. oop@ornl.gov
authors.forename: J
authors.initials: J
authors.lastname: Woodward
authors.forename: S M
authors.initials: SM
authors.lastname: Mattingly
authors.forename: M
authors.initials: M
authors.lastname: Danson
authors.forename: D
authors.initials: D
authors.lastname: Hough
authors.forename: N
authors.initials: N
authors.lastname: Ward
authors.forename: M
authors.initials: M
authors.lastname: Adams
mesh_terms: D003001:Cloning, Molecular; D004926:Escherichia coli / Q000235:genetics; D005947:Glucose / Q000378:metabolism; D042843:Glucose 1-Dehydrogenase; D005948:Glucose Dehydrogenases / Q000235:genetics / Q000378:metabolism*; D006859:Hydrogen / Q000378:metabolism*; D006864:Hydrogenase / Q000378:metabolism*; D010084:Oxidation-Reduction; D019711:Pyrococcus / Q000201:enzymology; D011994:Recombinant Proteins / Q000378:metabolism; D013822:Thermoplasma / Q000201:enzymology
chemical_list: D011994:Recombinant Proteins; D006859:Hydrogen; D005948:Glucose Dehydrogenases; D042843:Glucose 1-Dehydrogenase; D006864:Hydrogenase; D005947:Glucose