Transcriptional regulation of the gltA and TLC genes in Rickettsia prowazekii growing in a respiration-deficient host cell.
The regulation of the citrate synthase (gltA) and ATP/ADP translocase (tlc) genes of the obligate intracellular bacterium, Rickettsia prowazekii, was analyzed in rickettsia-infected respiration-deficient G14 cells. The level of the gltA mRNAII and the tlc mRNA was much lower in the total RNA isolated from the infected G14 cells grown in 1 g/l glucose (low glucose, GL) medium than in that from infected G14 cells grown in 4.5 g/l glucose (high glucose, GH) medium. However, the level of the gltA mRNAI relative to 16 S rRNA was the same in GL and GH media. An increase in the level of the gltA mRNAII and the tlc mRNA could be observed as early as 2 hrs after shifting from GL to GH medium. We conclude that, under these experimental conditions, the tlc promoter and the gltA promoter P2, but not gltA promoter P1, were transcriptionally regulated.
Cai
J
J
Department of Microbiology and Immunology, University of South Alabama College of Medicine, Mobile 36688, USA.
Winkler
H H
HH
D000244:Adenosine Diphosphate / Q000378:metabolism; D000255:Adenosine Triphosphate / Q000378:metabolism; D000818:Animals; D002460:Cell Line; D002950:Citrate (si)-Synthase / Q000235:genetics* / Q000378:metabolism; D006224:Cricetinae; D003412:Cricetulus; D015964:Gene Expression Regulation, Bacterial; D005947:Glucose / Q000378:metabolism; D007700:Kinetics; D000226:Mitochondrial ADP, ATP Translocases / Q000235:genetics* / Q000378:metabolism; D010101:Oxygen Consumption; D011401:Promoter Regions, Genetic; D012329:RNA, Bacterial / Q000235:genetics / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D012283:Rickettsia prowazekii / Q000201:enzymology / Q000235:genetics* / Q000254:growth & development; D014158:Transcription, Genetic
D012329:RNA, Bacterial; D012333:RNA, Messenger; D000244:Adenosine Diphosphate; D000255:Adenosine Triphosphate; D000226:Mitochondrial ADP, ATP Translocases; D002950:Citrate (si)-Synthase; D005947:Glucose