Vaccinia virus-induced changes in cytokine-regulated acute phase plasma protein synthesis by hepatoma cells.
Human hepatoma cell line, HepG2, has been infected with vaccinia virus and synthesis of plasma proteins was determined by electroimmunoassay and corresponding mRNA's measured by Northern blotting. The inhibitory effect of the virus was dose- and time-dependent. Electrophoretic mobility shift assay revealed a decrease in C/EBP binding activities in nuclear extracts isolated from the infected hepatoma cells. Supershift analysis of the C/EBP isoforms showed alpha and beta subunit involvement in DNA binding. The treatment of the cells with interleukin-1, interleukin-6, and dexamethasone at the initial stage of infection appears to delay the virally induced inhibition of host cell protein synthesis. Thus, possible "protective" role of the acute phase cytokines in viral infection is proposed.
Rokita
H
H
Institute of Molecular Biology, Jagiellonian University, Kraków, Poland. hannar@mol.uj.edu.pl
Branicki
W
W
Wrońska
D
D
Borysiewicz
L K
LK
Koj
A
A
D000209:Acute-Phase Proteins / Q000096:biosynthesis*; D018931:Antineoplastic Agents, Hormonal / Q000494:pharmacology; D006528:Carcinoma, Hepatocellular / Q000378:metabolism* / Q000821:virology*; D003907:Dexamethasone / Q000494:pharmacology; D006801:Humans; D007375:Interleukin-1 / Q000494:pharmacology / Q000502:physiology*; D015850:Interleukin-6 / Q000494:pharmacology / Q000502:physiology*; D008113:Liver Neoplasms / Q000378:metabolism* / Q000821:virology*; D012333:RNA, Messenger / Q000378:metabolism; D014407:Tumor Cells, Cultured; D014615:Vaccinia / Q000378:metabolism; D014616:Vaccinia virus
D000209:Acute-Phase Proteins; D018931:Antineoplastic Agents, Hormonal; D007375:Interleukin-1; D015850:Interleukin-6; D012333:RNA, Messenger; D003907:Dexamethasone