Characterization of chemotactic responses and flagella of Hyphomicrobium strain W1-1B.
Motile swarmer cells of Hyphomicrobium strain W1-1B displayed positive chemotactic responses toward methylamine, dimethylamine, and trimethylamine but did not display significant chemotactic responses towards methanol and arginine. Electron micrographs of negatively stained intact flagellar filaments indicated a novel striated surface pattern. The flagella were composed of two proteins of 39 and 41 kDa. Neither protein was a glycoprotein as determined by Schiff's staining and by enzyme immunoassay. Protein fingerprints visualized from silver-stained polyacrylamide gels and Western blots of protease-digested samples indicated that the two proteins were similar but not identical. Monoclonal antibodies prepared to the complex flagella of Rhizobium meliloti cross-reacted with the striated flagella of Hyphomicrobium strain W1-1B; however, these antibodies did not cross-react with smooth-surface flagella. These results suggest that complex and striated flagella possess homologous epitope regions.
Tuhela
L
L
Department of Microbiology, The Ohio State University, Columbus, Ohio 43210-1292, USA.
Robinson
J B
JB
Tuovinen
O H
OH
D000907:Antibodies, Bacterial; D000911:Antibodies, Monoclonal; D000942:Antigens, Bacterial / Q000032:analysis; D001120:Arginine / Q000494:pharmacology; D001419:Bacteria / Q000166:cytology* / Q000648:ultrastructure; D002630:Chemotactic Factors / Q000494:pharmacology; D002633:Chemotaxis; D003429:Cross Reactions; D005407:Flagella / Q000737:chemistry / Q000648:ultrastructure*; D005408:Flagellin / Q000032:analysis; D000432:Methanol / Q000494:pharmacology; D008744:Methylamines / Q000494:pharmacology; D012697:Serine Endopeptidases
D000907:Antibodies, Bacterial; D000911:Antibodies, Monoclonal; D000942:Antigens, Bacterial; D002630:Chemotactic Factors; D008744:Methylamines; D005408:Flagellin; D001120:Arginine; D012697:Serine Endopeptidases; C028344:glutamyl endopeptidase; D000432:Methanol