Expression of glutathione-peroxidase (GSH-PO) in the rat ventral prostate--effect of castration and administration of testosterone.
Immunolocalization of glutathione-peroxidase (GSH-PO), apoptosis and bcl-2 protein in the rat ventral prostate was investigated in the presence or absence of androgen. Male Sprague-Dawley rats were divided into four experimental groups. Group 1 consisted of intact controls. In group 2, rats were sacrificed two days after castration. In groups 3 and 4, rats were administered subcutaneously 1 mg/animal of testosterone-propionate daily for three or seven days at two days after castration. The intensity of GSH-PO staining in the glandular epithelial cells of the ventral prostate was remarkably decreased after castration (Group 2), and it clearly recovered when testosterone was administered (Groups 3 and 4) to the castrated rats. The prostatic GSH-PO mRNA levels were diminished in the castrated rat ventral prostate but greatly increased by testosterone (Groups 3 and 4). Furthermore, castration (Group 2) induced apoptosis in the prostatic glandular epithelial calls and the apoptosis was reduced by testosterone-administration (Groups 3 and 4) to the castrated rats. In groups 3 and 4, expression of bcl-2 protein was clearly detected in the glandular epithelial cells of the ventral prostate. These findings strongly suggested that expression of GSH-PO and bcl-2 protein in the glandular epithelial cells of the rat ventral prostate is testosterone-dependent.
Murakoshi
M
M
Safety Research Department, Teikoku Hormone Mfg. Co., Ltd. Kanagawa, Japan.
Ikeda
R
R
Tagawa
M
M
Suzuki
M
M
Takekoshi
S
S
Watanabe
K
K
D000818:Animals; D017209:Apoptosis / Q000502:physiology; D015152:Blotting, Northern; D005979:Glutathione Peroxidase / Q000235:genetics / Q000378:metabolism*; D007150:Immunohistochemistry; D008297:Male; D009919:Orchiectomy; D011467:Prostate / Q000187:drug effects* / Q000201:enzymology*; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000378:metabolism; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D013739:Testosterone / Q000494:pharmacology*; D014018:Tissue Distribution
D019253:Proto-Oncogene Proteins c-bcl-2; D012333:RNA, Messenger; D013739:Testosterone; D005979:Glutathione Peroxidase