title: [Serologic cross-reactions to Leishmania infantum using indirect immunofluorescence in HIV+ and HIV- patients with active tuberculosis].
abstract: BACKGROUND
  Clinical presentation of disseminated tuberculosis and visceral leishmaniosis can be very similar, mainly in those infected with HIV, being serology a useful tool in making a differential diagnosis. Cross-reactions of IFAT serodiagnosis of visceral leishmaniosis with other diseases are well known, but few data is available with tuberculosis.
  
  
  RESULTS
  Detection of serum antibodies against Leishmania, using a commercial IFAT kit, was attempted in sera of 51 patients with active pulmonar and/or extrapulmonar tuberculosis (25 HIV+ and 26 HIV-). Overall cross-reactions was found in 19.6% patients without significative differences in between 2 groups, but differences in positive serum titres was observed: one at 1/256, three at 1/160, and one at 1/80 dilution, in the HIV+ group, whereas all 5 patients in HIV- group cross-reacted at 1/80 dilution. Recognition of specific leishmanial antigenic bands by serum antibodies of patients with tuberculosis were not clearly defined by Western-blot.
  
  
  CONCLUSIONS
  IFAT technique for leishmaniosis cross-react in 20% of patients with tuberculosis.
authors.affiliation: Medicina Tropical y Parasitología Clínica, Hospital Ramón y Cajal, Madrid.
authors.forename: R
authors.initials: R
authors.lastname: López-Vélez
authors.forename: M C
authors.initials: MC
authors.lastname: Turientes
authors.forename: E
authors.initials: E
authors.lastname: Gómez-Mampaso
mesh_terms: D017088:AIDS-Related Opportunistic Infections / Q000175:diagnosis / Q000276:immunology; D000818:Animals; D000907:Antibodies, Bacterial / Q000276:immunology*; D000913:Antibodies, Protozoan / Q000276:immunology*; D000942:Antigens, Bacterial / Q000276:immunology; D015153:Blotting, Western; D003429:Cross Reactions; D003937:Diagnosis, Differential; D005189:False Positive Reactions; D019084:Fluorescent Antibody Technique, Indirect; D006017:Glycolipids / Q000276:immunology; D006023:Glycoproteins / Q000276:immunology; D018023:HIV Seronegativity / Q000276:immunology*; D006679:HIV Seropositivity / Q000276:immunology*; D015497:HIV-1; D006801:Humans; D018314:Leishmania infantum / Q000276:immunology*; D007898:Leishmaniasis, Visceral / Q000175:diagnosis / Q000276:immunology*; D008565:Membrane Proteins / Q000276:immunology; D009169:Mycobacterium tuberculosis / Q000276:immunology*; D015800:Protozoan Proteins / Q000276:immunology; D011897:Random Allocation; D012698:Serologic Tests; D014376:Tuberculosis / Q000175:diagnosis / Q000276:immunology*
chemical_list: D000907:Antibodies, Bacterial; D000913:Antibodies, Protozoan; D000942:Antigens, Bacterial; D006017:Glycolipids; D006023:Glycoproteins; D008565:Membrane Proteins; D015800:Protozoan Proteins
vernacular_title: Reacciones cruzadas de la serología a Leishmania infantum por inmunofluorescencia indirecta en pacientes HIV+ y HIV- con tuberculosis activa.