Expression of peroxisome proliferator-activated receptor alpha (PPAR alpha) in primary cultures of human vascular endothelial cells.
The peroxisome proliferator-activated receptor (PPAR) is a member of the steroid nuclear receptor superfamily. Three types of PPARs have been described in humans: PPAR alpha, PPAR beta, and PPAR gamma. Here we investigated the levels of PPAR alpha mRNA in primary cultures of human umbilical venous endothelial cells (HUVEC), human umbilical arterial endothelial cells (HUAEC), human coronary arterial endothelial cells (HCAEC), and human aortic endothelial cells (HAEC), using the reverse transcriptase-polymerase chain reaction (RT-PCR). The HUVEC, HAEC, and HCAEC, but not the HUAEC, showed relatively low expression of PPAR alpha in comparison with liver, which was used as a positive control. Moreover, the partial sequences of the PCR-amplified products from HUVEC, HAEC, and HCAEC were similar to that of the PPAR alpha from human liver. The expression of PPAR alpha in cultured HAEC, which were induced by dexamethasone, was inhibited by insulin. In addition, PPAR alpha expression was also increased by benzafibrate or eicosapentaenoic acid with the physiological concentration. These results suggest that the PPAR alpha in endothelial cells may have the same physiological role as the expression of PPAR alpha in the liver.
Inoue
I
I
Fourth Department of Medicine, Saitama Medical School, Japan.
Shino
K
K
Noji
S
S
Awata
T
T
Katayama
S
S
D001011:Aorta / Q000378:metabolism; D001629:Bezafibrate / Q000494:pharmacology; D002478:Cells, Cultured; D003331:Coronary Vessels / Q000378:metabolism; D003907:Dexamethasone / Q000494:pharmacology; D015118:Eicosapentaenoic Acid / Q000494:pharmacology; D004730:Endothelium, Vascular / Q000187:drug effects / Q000378:metabolism*; D015870:Gene Expression / Q000187:drug effects; D006801:Humans; D000960:Hypolipidemic Agents / Q000494:pharmacology; D007328:Insulin / Q000494:pharmacology; D008099:Liver / Q000378:metabolism; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D018160:Receptors, Cytoplasmic and Nuclear / Q000235:genetics*; D014157:Transcription Factors / Q000235:genetics*; D014469:Umbilical Arteries / Q000378:metabolism; D014471:Umbilical Veins / Q000378:metabolism
D000960:Hypolipidemic Agents; D007328:Insulin; D012333:RNA, Messenger; D018160:Receptors, Cytoplasmic and Nuclear; D014157:Transcription Factors; D003907:Dexamethasone; D015118:Eicosapentaenoic Acid; D001629:Bezafibrate