Alpha- and beta-globins of the anemic Belgrade laboratory rat. II. The effect of hemin and iron-dextran treatment.
We have studied the changes in bone marrow and spleen globin chain levels after in vivo hemin and iron-dextran treatment of hereditarily anemic Belgrade laboratory (b/b) rats. The increase of globin chains was detected in the bone marrow and in the spleen when b/b animals were treated with either iron or hemin. The analysis of changes in alpha- and beta-globin chain ratios revealed the distinctive role of these molecules in regulating globin chain status. Iron-dextran, as expected, ameliorated the imbalance of alpha- and beta-globin chains in the b/b rat spleen. On the other hand, hemin, as we have hypothesized in the accompanying paper, leads to a surplus of beta-globin chains in the bone marrow, similar to the one detected in the b/b rat spleen. Therefore, an iron-rich microenvironment has a stimulatory effect, while a hemin-rich microenvironment has an inhibitory effect on erythropoiesis.
Zarić
J
J
Institute of Molecular Genetics and Genetic Engineering, Belgrade, Yugoslavia.
Lazić
D
D
Marković
S
S
Glisin
V
V
Ivanović
Z
Z
Milenković
P
P
Popović
Z
Z
D000740:Anemia / Q000097:blood* / Q000188:drug therapy* / Q000235:genetics; D000818:Animals; D001853:Bone Marrow / Q000187:drug effects / Q000378:metabolism; D004912:Erythrocytes / Q000187:drug effects / Q000378:metabolism; D005260:Female; D005914:Globins / Q000096:biosynthesis / Q000235:genetics / Q000378:metabolism*; D006427:Hemin / Q000008:administration & dosage*; D006720:Homozygote; D007274:Injections, Intraperitoneal; D007505:Iron-Dextran Complex / Q000008:administration & dosage*; D008297:Male; D051381:Rats; D011922:Rats, Mutant Strains; D017208:Rats, Wistar; D013154:Spleen / Q000187:drug effects / Q000378:metabolism
D006427:Hemin; D005914:Globins; D007505:Iron-Dextran Complex