title: The DNA methylation locus DDM1 is required for maintenance of gene silencing in Arabidopsis.
abstract: To investigate the relationship between cytosine methylation and gene silencing in Arabidopsis, we constructed strains containing the ddm1 hypomethylation mutation and a methylated and silenced PAI2 tryptophan biosynthetic gene (MePAI2) that results in a blue fluorescent plant phenotype. The ddm1 mutation had both an immediate and a progressive effect on PAI gene silencing. In the first generation, homozygous ddm1 MePAI2 plants displayed a weakly fluorescent phenotype, in contrast to the strongly fluorescent phenotype of the DDM1 MePAI2 parent. After two generations of inbreeding by self-pollination, the ddm1/ddm1 lines became nonfluorescent. The progressive loss of fluorescence correlated with a progressive loss of methylation from the PAI2 gene. These results indicate that methylation is necessary for maintenance of PAI gene silencing and that intermediate levels of DNA methylation are associated with intermediate gene silencing. The results also support our earlier hypothesis that ddm1 homozygotes act as "epigenetic mutators" by accumulating heritable changes in DNA methylation that can lead to changes in gene expression.
authors.affiliation: Washington University, Department of Biology, St. Louis, Missouri 63130 USA.
authors.forename: J A
authors.initials: JA
authors.lastname: Jeddeloh
authors.forename: J
authors.initials: J
authors.lastname: Bender
authors.forename: E J
authors.initials: EJ
authors.lastname: Richards
mesh_terms: D017360:Arabidopsis / Q000235:genetics*; D001483:Base Sequence; D019175:DNA Methylation; D018744:DNA, Plant / Q000235:genetics*; D018506:Gene Expression Regulation, Plant; D017343:Genes, Plant; D008969:Molecular Sequence Data
chemical_list: D018744:DNA, Plant