Immunodot detection of nisin Z in milk and whey using enhanced chemiluminescence.
A highly specific antisera was produced in New Zealand white rabbits against nisin Z, a 3400 Da bacteriocin produced by Lactococcus lactis ssp. lactis biovar. diacetylactis UL 719. A dot immunoblot assay was then developed to detect nisin Z in milk and whey. As few as 1.5 10(-1) international units per ml (IU ml-1), corresponding to 0.003 microgram ml-1 of pure nisin Z, were detected in carbonate-bicarbonate buffer within 6 h using chemiluminescence. When milk and whey samples were tested, approximately 0.155 microgram ml-1 (7.9 IU ml-1) of nisin Z was detected. The detection limit obtained was lower than that of traditional methods including microtitration and agar diffusion.
Bouksaim
M
M
Centre de recherche en sciences et technologie du lait (STELA), Université Laval, Ste-Foy, Québec, Canada.
Fliss
I
I
Meghrous
J
J
Simard
R
R
Lacroix
C
C
D000818:Animals; D000900:Anti-Bacterial Agents / Q000032:analysis* / Q000276:immunology; D007124:Immunoenzyme Techniques; D008163:Luminescent Measurements; D008826:Microbial Sensitivity Tests; D008892:Milk / Q000737:chemistry*; D008894:Milk Proteins / Q000737:chemistry; D009561:Nisin / Q000031:analogs & derivatives* / Q000032:analysis / Q000276:immunology; D012680:Sensitivity and Specificity
D000900:Anti-Bacterial Agents; D008894:Milk Proteins; D009561:Nisin; C071364:nisin Z