Photographic detection of fluorescent-labelled oligodeoxynucleotide in the blotting format by peroxyoxalate chemiluminescence.
The preparation of a fluorescent labelled oligonucleotide and its photographic detection by peroxyoxalate chemiluminescence (PO-CL) are described. Fluorescent labelling of an oligonucleotide (15-mer) was performed with naphthalene-2,3-dicarboxaldehyde to give an N-substituted 1-cyanobenz[f]isoindole (CBI) derivative (CBI-15-mer). For the photographic detection of CBI-15-mer, the bis(2,6-difluorophenyl) oxalate (DFPO)-dimethyl phthalate (DMP) system was selected to obtain a long-lived CL emission. After optimizing the conditions for the CL reaction, the system was applied to the photographic detection, and as little as 250 fmol per spot of CBI-15-mer on a membrane were detected as a visible spot with an instant photographic film.
Kuroda
N
N
School of Pharmaceutical Sciences, Nagasaki University, Japan.
Hosoki
S
S
Nakashima
K
K
Akiyama
S
S
Givens
R
R
D001483:Base Sequence; D005456:Fluorescent Dyes; D007202:Indicators and Reagents; D008163:Luminescent Measurements; D009281:Naphthalenes; D009838:Oligodeoxyribonucleotides / Q000737:chemistry*; D010070:Oxalates; D010781:Photography
D005456:Fluorescent Dyes; D007202:Indicators and Reagents; D009281:Naphthalenes; D009838:Oligodeoxyribonucleotides; D010070:Oxalates; C044622:peroxyoxalate; C027717:2,3-naphthalenedicarboxaldehyde