Enhanced hybridization labeling signals in Southern blotted DNAs fractionated with voltage gradient gel electrophoresis.
An enhancement of hybridization labeling signals is demonstrated in Southern blotted DNAs, fractionated by voltage gradient gel electrophoresis. This enhancement is due to a reduced thickness of each single nucleic acid band in the gel as a consequence of the gradient effect, corresponding to an increased concentration of DNA per unit area.
Barbieri
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Dipartimento di Biologia Cellulare e dello Sviluppo, Università di Palermo, Italy.
Duro
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Izzo
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D000818:Animals; D015139:Blotting, Southern / Q000379:methods*; D005591:Chemical Fractionation; D004247:DNA / Q000737:chemistry*; D004563:Electrochemistry; D004587:Electrophoresis, Agar Gel / Q000379:methods*; D008565:Membrane Proteins / Q000235:genetics; D009693:Nucleic Acid Hybridization; D012617:Sea Urchins
C065555:Bep4 protein, Paracentrotus lividus; D008565:Membrane Proteins; D004247:DNA