title: Binding of platinum-diaminonitroxyl complexes to animal DNA.
abstract: Reaction of PtII(DAPO)X2 complexes (where DAPO is trans-3,4-diamino-2,2,6,6-tetramethylpiperidine-1-oxyl, X2 = (NO3)2, oxalato (Ox) or 1,1-cyclobutanedicarboxylato (Cbdca)) with a bovine spleen DNA in 0.01 M NaHCO3 at 37 degrees C for 24 h gives rise to formation of platinated DNA. The [bound PtII(DAPO)]/[nucleotide] ratio (r) depends on the initial ratio of the reagents and on the nature of leaving ligands X. Nitroxyl-nitroxyl distances in platinated DNA determined by the ESR suggest that at r > or = 0.1 PtII(DAPO) fragments are uniformly attached to DNA. But at lower r, the thermal characteristics of modified DNA (melting temperature Tm, melting range width delta T) and the guanine-to-adenine platination degree ratios GPt/APt imply that the nature of leaving ligands X affect the selectivity of DNA platination. At r > or = 0.1, nitroxyl groups can approach each other so close that, in an acidic medium, the electron transfer from one nitroxyl group to another becomes possible, and the nitroxyls readily disproportionate to diamagnetic products. Correlation time of nitroxyl rotation in PtII(DAPO)-DNA adducts is approximately 10(-8) s, which is related to predominantly bifunctional bonding of PtII(DAPO) with DNA. Platination-induced distortion of DNA was evidenced by changes in Tm, delta T and degree of hyperchromicity H. The major part of adducts form the intrastrand cross-links which destabilize the structure of DNA duplex. The interstrand PtII(DAPO) cross-linking (approximately 1% of the adducts) facilitates renaturation of despiralized DNA molecules upon cooling. Two types of PtII(DAPO)-DNA adducts are revealed, which differ substantially in their rates of deplatination with NaCN. ESR, electron spin resonance; r, degree of modification; cisplatin, cis-diamminedichloroplatinum(II); Tm, melting temperature; delta T, melting range width; H, degree of hyperchromicity; R, degree of renaturation; AAS, atomic absorption spectroscopy; HPLC, high performance liquid chromatography.
authors.affiliation: Institute of Chemical Physics in Chernogolovka, Russian Academy of Sciences, Moscow Region, Russian Federation.
authors.forename: A V
authors.initials: AV
authors.lastname: Shugalii
authors.forename: A V
authors.initials: AV
authors.lastname: Kulikov
authors.forename: M V
authors.initials: MV
authors.lastname: Lichina
authors.forename: V A
authors.initials: VA
authors.lastname: Golubev
authors.forename: V D
authors.initials: VD
authors.lastname: Sen
mesh_terms: D000818:Animals; D002417:Cattle; D002851:Chromatography, High Pressure Liquid; D002945:Cisplatin / Q000378:metabolism; D004247:DNA / Q000378:metabolism*; D004578:Electron Spin Resonance Spectroscopy; D006868:Hydrolysis; D046911:Macromolecular Substances; D008956:Models, Chemical; D009944:Organoplatinum Compounds / Q000378:metabolism*; D013154:Spleen / Q000378:metabolism; D013816:Thermodynamics
chemical_list: D046911:Macromolecular Substances; D009944:Organoplatinum Compounds; D004247:DNA; D002945:Cisplatin