title: Capability of neurite regeneration of retinal explant from adult rat after optic nerve injury.
abstract: Axons of the central nervous system in adult mammals do not regenerate spontaneously after axotomy. To understand whether the optic nerve of adult mammals loses the intrinsic capability to regenerate after injury, we have studied the capability of neurite regeneration of retinal explant from adult rat after optic nerve axotomy in vitro. After experimental blunt damage to the optic nerve of adult Wistar rat, the retinal explants from three days, one week, two weeks and three weeks after axotomy were put in tissue culture to observe the neurite growth after four days' incubation. The neurites were identified as retinal neuron origin by immunocytochemical staining using monoclonal antibody to neurofilament. The results demonstrate that retinal explants from adult rat after optic nerve damage have the capability of neurite regeneration; the capability is strongest in the group of one week after axotomy of optic nerve, but it decreases with passage of the time. On the other hand, the retinal explant from the control group of uninjured eye does not regenerate neurite in tissue culture. These results indicate that the retinal explant of adult rat has intrinsic capability to regenerate after optic nerve injury in vitro, and the capability of neurite regeneration decreases after one week post-trauma.
authors.affiliation: Department of Ophthalmology, School of Medicine, Kaohsiung Medical College, Taiwan, Republic of China.
authors.forename: R K
authors.initials: RK
authors.lastname: Tsai
authors.forename: H Z
authors.initials: HZ
authors.lastname: Wang
authors.forename: M M
authors.initials: MM
authors.lastname: Sheu
mesh_terms: D000818:Animals; D009416:Nerve Regeneration; D016501:Neurites / Q000502:physiology*; D020221:Optic Nerve Injuries; D051381:Rats; D017208:Rats, Wistar; D012160:Retina / Q000502:physiology*