FtsI and FtsW are localized to the septum in Escherichia coli.
The localization of FtsI (PBP3), a penicillin-binding protein specifically required for cell division in Escherichia coli, was investigated by immunofluorescence microscopy and found to localize to the septum. The localization of FtsI was not observed in ftsZ or ftsA mutants, indicating that it was dependent on the prior localization of these proteins. Addition of furazlocillin, a specific inhibitor of FtsI, prevented localization of FtsI even though FtsZ and FtsA localization occurred. Interestingly, the localization of FtsN was also prevented by furazlocillin. FtsZ displayed limited localization in furazlocillin-treated cells, whereas it was efficiently localized in FtsI-depleted cells. FtsW, another essential cell division protein, was also localized to the septum.
Wang
L
L
Department of Microbiology, Molecular Genetics and Immunology, University of Kansas Medical Center, Kansas City, Kansas 66216, USA.
Khattar
M K
MK
Donachie
W D
WD
Lutkenhaus
J
J
D001390:Azlocillin / Q000031:analogs & derivatives / Q000494:pharmacology; D001426:Bacterial Proteins / Q000032:analysis* / Q000235:genetics; D002352:Carrier Proteins; D002455:Cell Division / Q000187:drug effects; D003598:Cytoskeletal Proteins; D004926:Escherichia coli / Q000737:chemistry* / Q000235:genetics; D029968:Escherichia coli Proteins; D006602:Hexosyltransferases / Q000032:analysis* / Q000037:antagonists & inhibitors; D048289:Imidazolidines; D008565:Membrane Proteins; D009097:Multienzyme Complexes / Q000032:analysis* / Q000037:antagonists & inhibitors; D002267:Muramoylpentapeptide Carboxypeptidase; D009154:Mutation; D046915:Penicillin-Binding Proteins; D010406:Penicillins / Q000494:pharmacology; D010458:Peptidyl Transferases / Q000032:analysis* / Q000037:antagonists & inhibitors
D001426:Bacterial Proteins; D002352:Carrier Proteins; D003598:Cytoskeletal Proteins; D029968:Escherichia coli Proteins; C065103:FtsA protein, Bacteria; C489310:FtsA protein, E coli; C071525:FtsZ protein, Bacteria; D048289:Imidazolidines; D008565:Membrane Proteins; D009097:Multienzyme Complexes; D046915:Penicillin-Binding Proteins; D010406:Penicillins; C061684:FtsW protein, Bacteria; D010458:Peptidyl Transferases; D006602:Hexosyltransferases; D002267:Muramoylpentapeptide Carboxypeptidase; D001390:Azlocillin; C017976:furazlocillin