Mode of action of torilin in multidrug-resistant cancer cell lines.
The mechanism of action of multidrug-resistance reversing activity of torilin was studied. In vitro experiments for the accumulation and efflux of vinblastine clearly indicated that MDR reversing effects of torilin would directly be associated with the increase of the intracellular accumulation of anticancer drugs by blocking the drug efflux. Furthermore, torilin increased the membrane ATPase activity from KB-V1 cells, suggesting that torilin might function by inhibiting drug transport mediated by P-glycoprotein.
Kim
S E
SE
Natural Product Biosynthesis RU., Korea Research Institute of Bioscience and Biotechnology, Yusong, Taejon, Korea.
Hong
Y S
YS
Kim
Y C
YC
Lee
J J
JJ
D020168:ATP Binding Cassette Transporter, Subfamily B, Member 1 / Q000378:metabolism; D000251:Adenosine Triphosphatases / Q000378:metabolism; D000972:Antineoplastic Agents, Phytogenic / Q000378:metabolism / Q000494:pharmacology; D001692:Biological Transport; D002462:Cell Membrane / Q000201:enzymology; D018432:Drug Resistance, Multiple; D019008:Drug Resistance, Neoplasm; D004357:Drug Synergism; D006801:Humans; D012717:Sesquiterpenes / Q000494:pharmacology*; D045789:Sesquiterpenes, Guaiane; D014407:Tumor Cells, Cultured; D014700:Verapamil / Q000378:metabolism / Q000494:pharmacology; D014747:Vinblastine / Q000378:metabolism / Q000494:pharmacology
D020168:ATP Binding Cassette Transporter, Subfamily B, Member 1; D000972:Antineoplastic Agents, Phytogenic; D012717:Sesquiterpenes; D045789:Sesquiterpenes, Guaiane; D014747:Vinblastine; D014700:Verapamil; D000251:Adenosine Triphosphatases; C112694:torilin