Determination of the amino acid neurotransmitters in the dorsal root ganglion of the rat by capillary electrophoresis with a laser-induced fluorescence-charge coupled device.
A sensitive, rapid and accurate analysis of the amino acid neurotransmitters in dorsal root ganglion (DRG) of rat was accomplished by capillary electrophoresis with a laser-induced fluorescence-charged coupled device (LIF-CCD) and fluorescein isothiocyanate (FITC) derivatization. Since the CCD is an image detector with a matrix array of photoelements, the photogenerated charge generated from single photoelements was summed up by on-chip charge binning, and used to improve the detection sensitivity. A self-compiled data processing program was used to calculate digitally the summation of the multiwavelength data and transform three-dimensional electropherograms into two-dimensional electropherograms. Use of this program improved the detection sensitivity, the extraction of useful statistical data and the performance of the quantitative analysis. Owing to its excellent detectability and the unique characteristic of its two-dimensional format, which allows to perform analog and digital summation, high detection sensitivity with detection limits ranging from 1.2 to 17.2 nM was obtained for the amino acid neurotransmitters by LIF-CCD. This method was demonstrated to be a powerful tool for analyzing the complex biological samples through quantitative determination of the six amino acid neurotransmitters in the DRG of rat.
Zhang
L
L
Department of Chemistry, Wuhan University, China.
Chen
H
H
Hu
S
S
Cheng
J
J
Li
Z
Z
Shao
M
M
D000818:Animals; D001330:Electronic Data Processing; D019075:Electrophoresis, Capillary; D016650:Fluorescein-5-isothiocyanate; D005727:Ganglia, Spinal / Q000737:chemistry*; D007202:Indicators and Reagents; D007834:Lasers; D018377:Neurotransmitter Agents / Q000032:analysis*; D051381:Rats; D012984:Software; D013050:Spectrometry, Fluorescence
D007202:Indicators and Reagents; D018377:Neurotransmitter Agents; D016650:Fluorescein-5-isothiocyanate