Construction of a umuC'-luxAB plasmid for the detection of mutagenic DNA repair via luminescence.
This paper describes a novel system for the detection of mutagenic DNA repair in Escherichia coli. The DNA damage inducible umuC gene of Escherichia coli has been fused to the luxAB genes from Vibrio harvleyi that encode the enzyme luciferase. Mutagenicity has been assessed semi-quantitatively by the induction of bioluminescence. This system is simple, rapid and equivalent in sensitivity to other currently available test procedures. Its use in the detection of known SOS mutagens MMS, MNNG and UV is described.
Justus
T
T
School of Biological Sciences, The Flinders University of South Australia, Adelaide, Australia.
Thomas
S M
SM
D019677:Artificial Gene Fusion; D001426:Bacterial Proteins / Q000235:genetics*; D004260:DNA Repair; D004259:DNA-Directed DNA Polymerase; D004926:Escherichia coli / Q000235:genetics*; D029968:Escherichia coli Proteins; D005798:Genes, Bacterial; D017930:Genes, Reporter; D007700:Kinetics; D008163:Luminescent Measurements; D008741:Methyl Methanesulfonate / Q000494:pharmacology; D008769:Methylnitronitrosoguanidine / Q000494:pharmacology; D009153:Mutagens / Q000494:pharmacology; D010957:Plasmids; D013014:SOS Response, Genetics; D014466:Ultraviolet Rays; D014733:Vibrio / Q000235:genetics
D001426:Bacterial Proteins; D029968:Escherichia coli Proteins; D009153:Mutagens; D008769:Methylnitronitrosoguanidine; C078202:UmuC protein, E coli; D008741:Methyl Methanesulfonate; D004259:DNA-Directed DNA Polymerase