title: Cytolysis of eutopic and ectopic endometrial cells by peripheral blood monocytes and peritoneal macrophages in women with endometriosis.
abstract: OBJECTIVE
  To compare the ability of peripheral blood monocytes (PBM) and peritoneal macrophages (PM) to mediate the in vitro cytolysis of endometrial cells from eutopic and ectopic endometrium in women with endometriosis.
  
  
  METHODS
  Prospective study of immune function.
  
  
  METHODS
  Institute for the Study and Treatment of Endometriosis and university-based research laboratories.
  
  
  METHODS
  Twenty-four women with endometriosis (15 in stage I/II, 9 in stage III/IV) and 4 patients treated with GnRH agonists.
  
  
  METHODS
  Peritoneal fluid and peripheral blood were sampled and eutopic and ectopic endometrium were biopsied during diagnostic laparoscopy.
  
  
  METHODS
  Lysis of autologous endometrial cells.
  
  
  RESULTS
  Peripheral blood monocytes were significantly more cytolytic than peritoneal macrophages against autologous uterine endometrial cells. However, PBM and PM displayed a similar degree of cytolysis against a hepatoma cell line. Ectopic endometrial cells were significantly more resistant to cytolysis by autologous PBMC than were matched eutopic endometrial cells, and were completely resistant to cytolysis by autologous PM.
  
  
  CONCLUSIONS
  The reduced capacity of PM from women with endometriosis to mediate the destruction of endometrial cells coupled with the increased resistance of ectopic endometrial cells to macrophage-mediated cytolysis may facilitate the survival of these cells within the peritoneal cavity of women with endometriosis.
authors.affiliation: Institute for the Study and Treatment of Endometriosis, and Rush Medical College, Chicago, Illinois 60612, USA.
authors.forename: D P
authors.initials: DP
authors.lastname: Braun
authors.forename: H
authors.initials: H
authors.lastname: Gebel
authors.forename: N
authors.initials: N
authors.lastname: Rana
authors.forename: W P
authors.initials: WP
authors.lastname: Dmowski
mesh_terms: D000328:Adult; D003602:Cytotoxicity, Immunologic / Q000502:physiology*; D004715:Endometriosis / Q000473:pathology* / Q000503:physiopathology*; D004717:Endometrium / Q000473:pathology* / Q000503:physiopathology*; D005260:Female; D006801:Humans; D017737:Macrophages, Peritoneal / Q000502:physiology*; D009000:Monocytes / Q000502:physiology*; D011446:Prospective Studies; D014407:Tumor Cells, Cultured / Q000502:physiology