Influence of different uropathogenic microorganisms on human sperm motility parameters in an in vitro experiment.
The influence of different uropathogenic microorganisms (E. coli, enterococcus, Pseudomonas aeruginosa, Staphylococcus saprophyticus, Candida albicans) on human sperm motility was studied in vitro with a computer-assisted sperm analyser (CASA). Native ejaculates were prepared with the swim-up technique and adjusted to 22 x 10(6) spermatozoa ml-1. The sperm suspension was artificially infected with microorganisms in concentrations varying from 2 x 10(3) to 2 x 10(7). Sperm motility was examined directly after incubation, 2, 4 and 6 h later using the Mika motion analysis, a computer-based, automatic motility analysis. Former results with E. coli (serotype 06) could be confirmed that a significant inhibitory effect on sperm motility was associated with bacterial growth. Experiments with the enterococcus strain and Staphylococcus saprophyticus indicated no significant influence on sperm motility parameters. Tests with Pseudomonas aeruginosa showed a decrease of progressive motility according to time, but not to different bacterial concentrations. A significant inhibitory effect of Candida albicans was only detected in the samples with the initial bacterial concentration of 2 x 10(7) microorganisms ml-1.
Huwe
P
P
Department of Urology, Justus Liebig University, Giessen, Germany.
Diemer
T
T
Ludwig
M
M
Liu
J
J
Schiefer
H G
HG
Weidner
W
W
D001419:Bacteria / Q000472:pathogenicity*; D001424:Bacterial Infections / Q000150:complications / Q000382:microbiology / Q000503:physiopathology; D002176:Candida albicans / Q000472:pathogenicity; D002177:Candidiasis / Q000150:complications / Q000382:microbiology / Q000503:physiopathology; D016983:Enterococcus / Q000472:pathogenicity; D004926:Escherichia coli / Q000472:pathogenicity; D006801:Humans; D066298:In Vitro Techniques; D007248:Infertility, Male / Q000209:etiology / Q000382:microbiology / Q000503:physiopathology; D008297:Male; D052801:Male Urogenital Diseases / Q000150:complications / Q000382:microbiology / Q000503:physiopathology; D011550:Pseudomonas aeruginosa / Q000472:pathogenicity; D013081:Sperm Motility; D013210:Staphylococcus / Q000472:pathogenicity