Attachment of Cryptosporidium parvum sporozoites to human intestinal epithelial cells.
An enzyme-linked immunosorbent assay-based attachment model using the human intestinal cell line Caco-2A was developed to study attachment of Cryptosporidium parvum sporozoites in vitro and to assess potential inhibitors of sporozoite binding. In this system, attachment was related to sporozoite dose, incubation time, and host cell differentiation status. Polyclonal antibodies to C. parvum as well as glycoprotein inhibitors of a sporozoite lectin reduced attachment. This model will be a valuable tool in elucidating specific molecules and mechanisms involved in sporozoite-host cell attachment.
Joe
A
A
Division of Geographic Medicine and Infectious Diseases, New England Medical Center, Tufts University School of Medicine, Boston, Massachusetts 02111, USA.
Verdon
R
R
Tzipori
S
S
Keusch
G T
GT
Ward
H D
HD
D000268:Adhesiveness; D000818:Animals; D000913:Antibodies, Protozoan / Q000276:immunology; D018938:Caco-2 Cells; D016785:Cryptosporidium parvum / Q000737:chemistry / Q000276:immunology / Q000502:physiology*; D004797:Enzyme-Linked Immunosorbent Assay; D005455:Fluorescent Antibody Technique; D006023:Glycoproteins / Q000032:analysis; D006801:Humans; D013997:Time Factors
D000913:Antibodies, Protozoan; D006023:Glycoproteins