[Evaluation of a complex trace element composition and bioutilization using isotope technics and total body measurement].
Modified mineral and trace element solutions were prepared containing Zn-65, Co-57, Mn-54, Fe-59, Mo-99 and Ni-63 isotopes which were physico-chemically identical to the original solution. Bioutilization examinations were carried out on animals receiving their normal feeding, after p. os application of complex trace element composition (CTEC) namely whole-body retention studies, bioassays, scintigraphic and excretion examinations in altogether 180 Wistar rats, 6 Beagle and 2 mongrel dogs using whole body counter, gamma and beta counters, gamma camera and metabolic cages. Extremely high whole body retention was measured in case of iron (8-30%), high utilizations in case of zinc (4-5%), cobalt (4-6%), molybdenum (3-4%) and manganese (2-4%) and a lower value in case of nickel. Bioassay and scintigraphic evaluations showed marked liver-, kidney-, and muscle and moderated blood uptakes. The way of excretion was mainly (more than 90%) via the faeces in case of zinc, manganese, iron and nickel, although cobalt excreted in 8% and molybdenum in 52% via the urinary tract. Our results show, that isotope technique combined with whole body counting and excretion studies in an available method for trace element bioutilization studies.
Balogh
L
L
Országos Frédéric Joliot-Curie Sugárbiológiai és Sugáregészségügyi Kutató Intézet, Budapest.
Kerekes
A
A
Bodó
K
K
Körösi
L
L
Jánoki
G A
GA
D000818:Animals; D001681:Biological Assay; D003300:Copper / Q000378:metabolism; D004285:Dogs; D006801:Humans; D007501:Iron / Q000378:metabolism; D011868:Radioisotopes; D051381:Rats; D014131:Trace Elements / Q000378:metabolism / Q000494:pharmacology; D015032:Zinc / Q000378:metabolism
D011868:Radioisotopes; D014131:Trace Elements; D003300:Copper; D007501:Iron; D015032:Zinc
Komplex nyomelem készítmény biológiai hasznosulásának tanulmányozása izotópos nyomjelzéstechnika és egésztest-mérés alkalmazásával.