Physical map of the genome of Oenococcus oeni PSU-1 and localization of genetic markers.
A physical map of the chromosome of Oenococcus oeni PSU-1 was constructed. This represents the first map for a strain of this species. A total of 37 restriction sites for the rare-cutting endonucleases Ascl, Fsel, Notl and Sfil were mapped on the chromosome, which was found to be circular with an estimated size of 1857 kb. Fragment order was determined using several approaches: analysis of partial and double digestions, two-dimensional pulsed-field gel electrophoresis, isolation of linking clones, and Southern hybridization with labelled restriction fragments both from PSU-1 and from O. oeni strain GM. Oenococcal genes alsS/alsD, mleA and mir, two phage attachment sites and recurrent sequences such as IS1165-like elements and rrn loci were located on the physical map. Specific fragments hybridizing with gene probes from Lactococcus lactis, Leuconostoc mesenteroides and Bacillus subtilis were also identified. The two ribosomal operons have been precisely located and their transcription direction determined.
Zé-Zé
Líbia
L
Centro de Genética e Biologia Molecular, UL, 1700 Lisboa, Portugal.|Departamento de Biologia Vegetal, Faculdade de Ciências da Universidade de Lisboa, Edifício C2, Piso 4, Campo Grande, 1700 Lisboa, Portugal.
Tenreiro
Rogério
R
Instituto de Tecnologia Química e Biológica, 2780 Oeiras, Portugal.|Centro de Genética e Biologia Molecular, UL, 1700 Lisboa, Portugal.|Departamento de Biologia Vegetal, Faculdade de Ciências da Universidade de Lisboa, Edifício C2, Piso 4, Campo Grande, 1700 Lisboa, Portugal.
Brito
Luisa
L
Instituto de Tecnologia Química e Biológica, 2780 Oeiras, Portugal.
Santos
Mário A
MA
Instituto de Tecnologia Química e Biológica, 2780 Oeiras, Portugal.|Departamento de Biologia Vegetal, Faculdade de Ciências da Universidade de Lisboa, Edifício C2, Piso 4, Campo Grande, 1700 Lisboa, Portugal.
Paveia
Helena
H
Centro de Genética e Biologia Molecular, UL, 1700 Lisboa, Portugal.|Departamento de Biologia Vegetal, Faculdade de Ciências da Universidade de Lisboa, Edifício C2, Piso 4, Campo Grande, 1700 Lisboa, Portugal.
D001435:Bacteriophages / Q000235:genetics; D015139:Blotting, Southern; D002874:Chromosome Mapping; D002876:Chromosomes, Bacterial / Q000235:genetics*; D004262:DNA Restriction Enzymes; D004269:DNA, Bacterial / Q000235:genetics; D016521:Electrophoresis, Gel, Pulsed-Field; D005798:Genes, Bacterial; D005819:Genetic Markers; D006095:Gram-Positive Cocci / Q000235:genetics*; D007934:Leuconostoc / Q000235:genetics; D009693:Nucleic Acid Hybridization; D009876:Operon / Q000235:genetics; D015183:Restriction Mapping
D004269:DNA, Bacterial; D005819:Genetic Markers; D004262:DNA Restriction Enzymes