Stabilized, freeze-dried PCR mix for detection of mycobacteria.
We report here the development of a freeze-drying procedure allowing stabilization at ambient temperature of preoptimized, premixed, and predispensed PCR mixes aimed at the detection of mycobacteria in clinical materials. The freeze-dried mixes retained activity at 4 degrees C and at 20 degrees C for 1 year and for 3 months at 37 degrees C, as judged by their performance with 50 and 500 fg of purified Mycobacterium bovis BCG target DNA.
Klatser
P R
PR
Department of Biomedical Research, Royal Tropical Institute, Amsterdam, The Netherlands. BO@mail.support.nl
Kuijper
S
S
van Ingen
C W
CW
Kolk
A H
AH
D004269:DNA, Bacterial / Q000032:analysis; D004275:DNA, Ribosomal / Q000032:analysis; D005612:Freeze Drying; D009163:Mycobacterium bovis / Q000235:genetics* / Q000302:isolation & purification*; D016133:Polymerase Chain Reaction / Q000379:methods*; D012336:RNA, Ribosomal, 16S / Q000235:genetics
D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D012336:RNA, Ribosomal, 16S