The MTCY428.08 gene of Mycobacterium tuberculosis codes for NAD+ synthetase.
The product of the MTCY428.08 gene of Mycobacterium tuberculosis shows sequence homology with several NAD+ synthetases. The MTCY428.08 gene was cloned into the expression vectors pGEX-4T-1 and pET-15b. Expression in Escherichia coli led to overproduction of glutathione S-transferase fused and His6-tagged gene products, which were enzymatically assayed for NAD synthetase activity. Our results demonstrate that the MTCY428.08 gene of M. tuberculosis is the structural gene for NAD+ synthetase.
Cantoni
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R
Dipartimento di Genetica e Microbiologia, Università di Pavia, Italy.
Branzoni
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M
Labò
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M
Rizzi
M
M
Riccardi
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G
D000595:Amino Acid Sequence; D019733:Carbon-Nitrogen Ligases with Glutamine as Amide-N-Donor / Q000235:genetics* / Q000378:metabolism; D003001:Cloning, Molecular; D004926:Escherichia coli / Q000235:genetics; D005798:Genes, Bacterial; D008969:Molecular Sequence Data; D009169:Mycobacterium tuberculosis / Q000201:enzymology* / Q000235:genetics*; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000378:metabolism; D017386:Sequence Homology, Amino Acid
D011993:Recombinant Fusion Proteins; D019733:Carbon-Nitrogen Ligases with Glutamine as Amide-N-Donor; C063522:NAD+ synthase (glutamine-hydrolysing)