Simultaneous variation of temperature and gradient steepness for reversed-phase high-performance liquid chromatography method development. II. The use of further changes in conditions.
The preceding paper (Part I) suggests that simply optimizing temperature and gradient steepness will often provide an adequate reversed-phase HPLC separation. In some cases, however, this procedure will prove unsuccessful, and then further method-development experiments (involving change in other separation conditions) will be required. One strategy is to change a variable other than temperature or gradient steepness, followed by re-optimization of the latter two variables. The present paper examines the application of this approach with the aid of computer simulation to several samples.
Dolan
J W
JW
LC Resources Inc., Walnut Creek, CA 94596, USA.
Snyder
L R
LR
Saunders
D L
DL
Van Heukelem
L
L
D000097:Acetonitriles / Q000737:chemistry; D000179:Acrylates / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D003198:Computer Simulation; D004952:Esters / Q000737:chemistry; D056890:Eukaryota / Q000737:chemistry*; D005227:Fatty Acids / Q000032:analysis* / Q000737:chemistry; D006863:Hydrogen-Ion Concentration; D000432:Methanol / Q000737:chemistry; D008956:Models, Chemical; D009930:Organic Chemicals / Q000032:analysis* / Q000737:chemistry; D010860:Pigments, Biological / Q000032:analysis* / Q000737:chemistry; D012997:Solvents / Q000737:chemistry; D013256:Steroids / Q000032:analysis* / Q000737:chemistry; D013696:Temperature
D000097:Acetonitriles; D000179:Acrylates; D004952:Esters; D005227:Fatty Acids; D009930:Organic Chemicals; D010860:Pigments, Biological; D012997:Solvents; D013256:Steroids; D000432:Methanol; C032159:acetonitrile