Antisense mapping of opioid receptor clones: effects upon 2-deoxy-D-glucose-induced hyperphagia.
Antisense oligodeoxynucleotides (AS ODNs) directed against exons 1 and 2 of the MOR-1 clone significantly and markedly reduced (81-93%) hyperphagia induced by the anti-metabolic glucose analogue, 2-deoxy-d-glucose (2DG) across a 4 h time course. AS ODNs directed against exons 3 or 4 of the MOR-1 clone had a more limited (1-2 h) duration of action upon 2DG-induced hyperphagia. 2DG-induced hyperphagia was significantly reduced by AS ODNs directed against exon 2 (44-51%), but not exons 1 or 3 of the KOR-1 clone across a 4 h time course. Whereas an AS ODN probe directed against the KOR3/ORL-1 clone produced small (36%), but significant reductions in 2DG-induced hyperphagia, an AS ODN probe directed against the DOR-1 clone was ineffective. These data provide further converging evidence for the roles of primarily mu, but also kappa1 and kappa3 opioid receptors in mediating the hyperphagic effects of glucoprivation.
Burdick
K
K
Neuropsychology Doctoral Sub-Program, Queens College, CUNY, Flushing, NY 11367, USA.
Yu
W Z
WZ
Ragnauth
A
A
Moroz
M
M
Pan
Y X
YX
Rossi
G C
GC
Pasternak
G W
GW
Bodnar
R J
RJ
D000818:Animals; D003001:Cloning, Molecular; D003847:Deoxyglucose; D005091:Exons; D006963:Hyperphagia / Q000139:chemically induced*; D008297:Male; D016376:Oligonucleotides, Antisense; D051381:Rats; D017207:Rats, Sprague-Dawley; D011957:Receptors, Opioid / Q000032:analysis*
D016376:Oligonucleotides, Antisense; D011957:Receptors, Opioid; D003847:Deoxyglucose