title: Tissue distribution of hantavirus antigen in naturally infected humans and deer mice.
abstract: The Sin Nombre virus (SNV) is the etiologic agent of hantavirus pulmonary syndrome in humans but does not cause disease in chronically infected deer mice (Peromyscus maniculatus), the natural host. In this study, murine antiserum raised against recombinant SNV nucleocapsid protein was utilized to localize viral antigen immunohistochemically in tissues from both humans (n = 20; 11 positive, 9 negative) and deer mice (n = 6; 4 positive, 2 negative). Viral infection status was confirmed by Western blot or reverse transcriptase-polymerase chain reaction. SNV antigen was detected in pulmonary and cardiac endothelium in both species, but positive cells in deer mice were rare. Other deer mouse tissues, including kidney, were negative; in contrast, vascular elements of several tissues from infected humans were positive, with strong staining of renal endothelium. The paucity of positive cells in chronically infected mice suggests a low virus burden compared with that of acutely infected humans.
authors.affiliation: Department of Pathology, University of New Mexico School of Medicine, Albuquerque 87131, USA.
authors.forename: W
authors.initials: W
authors.lastname: Green
authors.forename: R
authors.initials: R
authors.lastname: Feddersen
authors.forename: O
authors.initials: O
authors.lastname: Yousef
authors.forename: M
authors.initials: M
authors.lastname: Behr
authors.forename: K
authors.initials: K
authors.lastname: Smith
authors.forename: J
authors.initials: J
authors.lastname: Nestler
authors.forename: S
authors.initials: S
authors.lastname: Jenison
authors.forename: T
authors.initials: T
authors.lastname: Yamada
authors.forename: B
authors.initials: B
authors.lastname: Hjelle
mesh_terms: D000818:Animals; D000956:Antigens, Viral / Q000276:immunology*; D002213:Capsid / Q000276:immunology*; D006476:Orthohantavirus / Q000235:genetics / Q000276:immunology*; D018804:Hantavirus Pulmonary Syndrome / Q000276:immunology* / Q000473:pathology / Q000662:veterinary*; D006801:Humans; D051379:Mice; D008807:Mice, Inbred BALB C; D010542:Peromyscus; D012376:Rodent Diseases / Q000276:immunology* / Q000473:pathology; D014018:Tissue Distribution
chemical_list: D000956:Antigens, Viral