title: Thiol compounds rescue growth inhibition by retinoic acid on HTLV-I (+) T lymphocytes; possible mechanism of retinoic-acid-induced growth inhibition of adult T-cell leukemia cells.
abstract: We demonstrated significant growth inhibition by retinoic acid (RA) of HTLV-I (+) T-cell lines (ATL-2 and HUT102), but not HTLV-I (-) T-cell lines (MOLT-4 and Jurkat). We hypothesized that the mechanism of growth inhibition by RA depends on an imbalance in redox potential. To examine the effect of exogenous thiol compounds for the growth of HTLV-I (+) T-cell lines by RA, HTLV-I (+) T-cell lines were cultured with several thiol compounds (thioredoxin, L-cystine, and GSH), following addition of 13-cis RA or ATRA, respectively, in cultured with thiol free medium. Unexpectedly, thiol compounds alone did not restore growth inhibition of HTLV-I (+) T-cell lines. However, when those cells were preincubated with thiol compounds for 24 hours, no growth inhibition by 13-cis RA or ATRA was observed. These results suggest that thiol compounds are associated strongly with sensitivity to RA of HTLV-I (+) T cells, but not of HTLV-I (-) T cells and that thiol compounds serve an important role on HTLV-I (+) T cells.
authors.affiliation: Third Department of Internal Medicine, Kinki University School of Medicine, Osaka, Japan.
authors.forename: J
authors.initials: J
authors.lastname: Miyatake
authors.forename: Y
authors.initials: Y
authors.lastname: Maeda
authors.forename: H
authors.initials: H
authors.lastname: Nawata
authors.forename: Y
authors.initials: Y
authors.lastname: Sumimoto
authors.forename: H
authors.initials: H
authors.lastname: Sono
authors.forename: M
authors.initials: M
authors.lastname: Sakaguchi
authors.forename: M
authors.initials: M
authors.lastname: Matsuda
authors.forename: Y
authors.initials: Y
authors.lastname: Tatsumi
authors.forename: F
authors.initials: F
authors.lastname: Urase
authors.forename: F
authors.initials: F
authors.lastname: Horiuchi
authors.forename: K
authors.initials: K
authors.lastname: Irimajiri
authors.forename: A
authors.initials: A
authors.lastname: Horiuchi
mesh_terms: D002455:Cell Division / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D003470:Culture Media; D006800:Deltaretrovirus Infections / Q000378:metabolism / Q000473:pathology*; D006131:Growth Inhibitors / Q000494:pharmacology*; D006801:Humans; D019169:Jurkat Cells; D015458:Leukemia, T-Cell / Q000378:metabolism / Q000473:pathology*; D013438:Sulfhydryl Compounds / Q000494:pharmacology*; D013601:T-Lymphocytes / Q000187:drug effects* / Q000473:pathology* / Q000821:virology; D014212:Tretinoin / Q000494:pharmacology*; D014407:Tumor Cells, Cultured
chemical_list: D003470:Culture Media; D006131:Growth Inhibitors; D013438:Sulfhydryl Compounds; D014212:Tretinoin