Effect on colon cancer cells of human interferon-beta gene entrapped in cationic multilamellar liposomes.
When cultured cells of human colon cancer cell line SW480 were transfected with human interferon-beta (hIFN-beta) gene by means of cationic multilamellar liposomes, the endogenously produced hIFN-beta exhibited a remarkable anti-proliferative effect on the cells, which was more effective than that of exogenously added hIFN-beta. This effect lasted for several days, and was blocked completely by the addition of sufficient amounts of anti-hIFN-beta antibody. From experiments using a transwell plate and an infusion pump, we found that endogenously produced hIFN-beta acted effectively on the cells around the transfectants and that the growth-inhibitory effect was totally retained upon continuous dilution of the medium. These data indicate that hIFN-beta expressed endogenously by transfer of its gene acted on these cancer cells mainly in a paracrine manner. Although the transfection with hIFN-gamma gene also revealed a definite growth-inhibitory effect on the same tumor cells, the extent was less than that of hIFN-beta gene.
Shimizu
M
M
Department of Surgery, Nagoya University School of Medicine, Japan.
Akiyama
S
S
Ito
K
K
Kasai
Y
Y
Takagi
H
H
Kito
M
M
Ohishi
N
N
Yagi
K
K
D000906:Antibodies / Q000494:pharmacology; D002455:Cell Division / Q000502:physiology; D003110:Colonic Neoplasms / Q000378:metabolism / Q000628:therapy*; D018076:DNA, Complementary / Q000008:administration & dosage* / Q000235:genetics; D015316:Genetic Therapy / Q000379:methods*; D006801:Humans; D016899:Interferon-beta / Q000096:biosynthesis / Q000235:genetics* / Q000378:metabolism; D007371:Interferon-gamma / Q000235:genetics; D008081:Liposomes; D014162:Transfection; D014407:Tumor Cells, Cultured
D000906:Antibodies; D018076:DNA, Complementary; D008081:Liposomes; D016899:Interferon-beta; D007371:Interferon-gamma