Mapping the antigenic structure of porcine parvovirus at the level of peptides.
The antigenic structure of the capsid proteins of porcine parvovirus (PPV) was investigated. A total of nine linear epitopes were identified by Pepscan using porcine or rabbit anti-PPV antisera. No sites were identified with a panel of neutralising monoclonal antibodies (MAbs). All epitopes were located in the region corresponding to the major capsid protein VP2. Based on this information, and on analogy to other autonomous parvoviruses, 24 different peptides were synthesised, coupled to keyhole limpet haemocyanin (KLH) and used to immunise rabbits. Most antisera were able to bind viral protein. Only peptides from the N-terminal part of VP2 were able to induce virus-neutralising antibodies, although at low levels. A similar neutralising activity could be obtained in pigs. The exposure of the N-terminus was shown in full virions, both by immunoelectron microscopy and absorption experiments. It is concluded that in PPV, the VP2 N-terminus is involved in virus neutralisation (VN) and peptides from this region are therefore primary targets for developing peptide-based vaccines against this virus.
Kamstrup
S
S
Danish Veterinary Institute for Virus Research, Kalvehave. sk@vetvirus.dk
Langeveld
J
J
Bøtner
A
A
Nielsen
J
J
Schaaper
W M
WM
Boshuizen
R S
RS
Casal
J I
JI
Højrup
P
P
Vela
C
C
Meloen
R
R
Dalsgaard
K
K
D000595:Amino Acid Sequence; D000818:Animals; D000914:Antibodies, Viral / Q000276:immunology; D000956:Antigens, Viral / Q000276:immunology; D002213:Capsid / Q000276:immunology*; D036022:Capsid Proteins; D004797:Enzyme-Linked Immunosorbent Assay; D018604:Epitope Mapping; D007114:Immunization; D008969:Molecular Sequence Data; D017992:Parvovirus / Q000276:immunology*; D010455:Peptides / Q000138:chemical synthesis / Q000276:immunology*; D011817:Rabbits; D013552:Swine / Q000821:virology*
D000914:Antibodies, Viral; D000956:Antigens, Viral; D036022:Capsid Proteins; D010455:Peptides