Activated protein C resistance assay as a screening test for thromboembolic disposition.
An activated partial thromboplastin time (APTT) based method was developed to determine the anticoagulant response in patient plasma to added purified activated protein C (APC). On the other hand, Bertina et al. (1994) reported that one point mutation of amino acid 506 in the factor V was observed in such APC resistance patients. APC resistance for blood samples with known abnormal findings on the coagulation test also may be determined. Coagmaster II for the APTT assay system (Sankyo, Tokyo, Japan) was employed. The APC resistance assay system was a useful reagent as a screening test for lupus anticoagulant (LA), in addition to APC resistance.
Shizuka
R
R
Department of Laboratory Medicine, Gunma University School of Medicine, Maebashi, Japan.
Amagai
H
H
Kojima
J
J
Fukumura
Y
Y
Kanda
T
T
Kobayashi
I
I
D002122:Calcium Chloride; D004351:Drug Resistance / Q000502:physiology; D005260:Female; D006801:Humans; D016682:Lupus Coagulation Inhibitor / Q000032:analysis; D008403:Mass Screening / Q000379:methods*; D010314:Partial Thromboplastin Time; D011486:Protein C / Q000502:physiology*; D011933:Reagent Kits, Diagnostic; D012016:Reference Values; D012680:Sensitivity and Specificity; D013923:Thromboembolism / Q000517:prevention & control*
D016682:Lupus Coagulation Inhibitor; D011486:Protein C; D011933:Reagent Kits, Diagnostic; D002122:Calcium Chloride