Recombinant bovine somatotropin (rbST) administration to creep-fed beef calves increases muscle mass but does not affect satellite cell number or concentration of myosin light chain-1f mRNA.
Our objective in this study was to determine the effect of recombinant bovine somatotropin (rbST) on indices of muscle development in creep-fed beef calves. Crossbred steer calves were assigned to one of two treatment groups: control (sham-injected; n = 12) or rbST-treated (.09 mg x kg(-1) x d(-1); n = 12). Calves were injected every 14 d starting at d 28 of age and were weaned at 205 d of age. Supplemental creep feed was supplied free access to all calves to compensate for an expected increased protein and energy requirement in calves given rbST. Biopsy (d 100) and slaughter (d 206) samples of semitendinosus muscle were evaluated for satellite cell, myofiber nuclei numbers, and myosin light chain (MLC-1f) mRNA quantification. Myofiber nuclei and satellite cell numbers per 100 myofibers and MLC-1f mRNA:rRNA ratios at 100 and 206 d of age were not different (P > .10) between control and rbST-treated calves. Total gain, ADG, quality grade, femur length, percentage kidney, pelvic, and heart fat, dressing percentage, plasma IGF-I, and plasma urea nitrogen concentrations did not differ (P > .10) between control and rbST-treated calves. However, rbST-treated calves had larger longissimus muscle areas (P < .03), less marbling (P < .001), higher carcass conformation scores (P < .04), greater mass of separated muscle (P < .03), more ground meat (P < .01), and heavier carcass weights (P < .05) than control calves. Thus, rbST treatment increased muscle characteristics while nuclei number and MLC-1f mRNA concentrations remained the same, implying that the additional muscle growth was in a normal fashion.
Vann
R C
RC
Department of Animal and Dairy Sciences, Mississippi State University, Mississippi State 39762, USA.
Althen
T G
TG
Smith
W K
WK
Veenhuizen
J J
JJ
Smith
S B
SB
D000821:Animal Feed; D000818:Animals; D001806:Blood Urea Nitrogen; D002417:Cattle / Q000254:growth & development*; D002452:Cell Count / Q000662:veterinary; D002467:Cell Nucleus / Q000187:drug effects; D013006:Growth Hormone / Q000008:administration & dosage / Q000494:pharmacology*; D007279:Injections, Subcutaneous / Q000662:veterinary; D007334:Insulin-Like Growth Factor I / Q000032:analysis / Q000187:drug effects; D008297:Male; D008460:Meat / Q000592:standards; D008938:Mitosis / Q000187:drug effects; D024510:Muscle Development; D018485:Muscle Fibers, Skeletal / Q000187:drug effects / Q000648:ultrastructure; D018482:Muscle, Skeletal / Q000166:cytology / Q000187:drug effects* / Q000254:growth & development; D018994:Myosin Light Chains / Q000187:drug effects / Q000235:genetics*; D012333:RNA, Messenger / Q000032:analysis* / Q000187:drug effects; D011994:Recombinant Proteins / Q000008:administration & dosage / Q000494:pharmacology; D013997:Time Factors; D015430:Weight Gain / Q000187:drug effects
D018994:Myosin Light Chains; D012333:RNA, Messenger; D011994:Recombinant Proteins; C076659:myosin light chain I; D007334:Insulin-Like Growth Factor I; D013006:Growth Hormone