Purification of PASII/PMP22--an extremely hydrophobic glycoprotein of PNS myelin membrane.
PASII/PMP22 protein (mol. wt 22 kDa, pI 8.8) is an abundant and extremely hydrophobic glycoprotein of PNS myelin which is solubilized effectively by SDS. In humans, this protein is involved in hereditary neuropathies. Here we describe a simple method for purification of PASII/PMP22, suitable for crystallization trials. We usually obtained 10-20 mg PASII/PMP22 from 10 g bovine spinal roots. It is notable, that the original protocol was designated for purification of P0 myelin glycoprotein, and purification of PASII/PMP22 is a bonus. Extensive crystallization trials are in progress.
Sedzik
J
J
Department of Physiology, Keio University School of Medicine, Tokyo, Japan.
Kotake
Y
Y
Uyemura
K
K
D000818:Animals; D002417:Cattle; D002846:Chromatography, Affinity; D003460:Crystallization; D004591:Electrophoresis, Polyacrylamide Gel; D007202:Indicators and Reagents; D009185:Myelin Proteins / Q000302:isolation & purification*; D009186:Myelin Sheath / Q000737:chemistry*; D013126:Spinal Nerve Roots / Q000737:chemistry*
D007202:Indicators and Reagents; D009185:Myelin Proteins