[Effect of glucose on the aqueous infrared spectrum (bloodless determination of blood glucose by tissue photometry)].
The creation of a noninvasive (without blood collection) method for measuring blood glucose concentrations, convenient for patients, is practically important, because the treatment of diabetics requires constant many-year monitoring of the alimentary component of glycemia. Use of IR spectra is impeded by the interference of strong bands of water absorption, whose concentration in the tissues is almost 1000 times higher than that of glucose. We investigated the effects of glucose, albumen, and blood serum on the structure of water absorption bands in the 1400-1600, 960, 1153, and 2000-2400 nm bands. The method of differentiated spectra was used. Glucose cleaved the negative aqueous band on the differential spectrum with 1410 and 1455 nm peaks and a positive 1590 nm peak with the isobestic point at 1540 nm. Albumen and serum proteins caused similar, although somewhat different effects, not preventing the use of the above wavelengths for the creation of a noninvasive device, because the concentrations of proteins and electrolytes are not changed after food intake if the homeostasis is intact.
Aristarkhov
V M
VM
Balakhovskiĭ
I S
IS
D000418:Albumins / Q000032:analysis; D001786:Blood Glucose / Q000032:analysis*; D001798:Blood Proteins / Q000032:analysis; D004435:Eating; D006706:Homeostasis; D006801:Humans; D012709:Serum Albumin / Q000032:analysis; D013050:Spectrometry, Fluorescence; D013055:Spectrophotometry, Infrared; D013059:Spectrum Analysis, Raman; D014867:Water
D000418:Albumins; D001786:Blood Glucose; D001798:Blood Proteins; D012709:Serum Albumin; D014867:Water
Vliianie gliukozy na infrakrasnyĭ spektr vody (k voporosu o beskrovnom opredelenii gliukozy v krovi metodom fotometrii tkaneĭ).