title: Successful pregnancy with sperm retrieved from the bladder after the introduction of a low-electrolyte solution for retrograde ejaculation.
abstract: OBJECTIVE
  To determine the safety and efficacy of the introduction of a low-electrolyte solution into the bladder before masturbation for sperm recovery in patients with retrograde ejaculation.
  
  
  METHODS
  Two case reports.
  
  
  METHODS
  Assisted reproduction practice at a university hospital.
  
  
  METHODS
  Two men with retrograde ejaculation.
  
  
  METHODS
  A 0.32-mol/L glucose solution or Ham's modified F-10 medium (GIBCO, Grand Island, NY) with 10% serum was introduced into the urinary bladder before masturbation. The retrieved sperm were used for IUI.
  
  
  METHODS
  The motility of retrieved sperm, achievement of pregnancy, and outcome of pregnancy.
  
  
  RESULTS
  In patient 1, the motility rate of sperm retrieved from the bladder after the introduction of Ham's modified F-10 medium was higher than that after the introduction of a glucose solution. The patient's wife did not become pregnant during 10 IUI cycles after treatment with Ham's modified F-10 medium. However, she became pregnant during the first IUI cycle after treatment with a glucose solution and was delivered of healthy twin infants. The wife of patient 2 also became pregnant during the first IUI cycle after treatment with a glucose solution and was delivered of a healthy female infant.
  
  
  CONCLUSIONS
  The introduction of a glucose solution appears to be a safe and simple method for sperm recovery in patients with retrograde ejaculation.
authors.affiliation: Department of Urology, Yokohama City University School of Medicine, Yokohama, Kanagawa, Japan. kazsaito@yellow.med.yokohama-cu.ac.jp
authors.forename: K
authors.initials: K
authors.lastname: Saito
authors.forename: Y
authors.initials: Y
authors.lastname: Kinoshita
authors.forename: Y
authors.initials: Y
authors.lastname: Yumura
authors.forename: A
authors.initials: A
authors.lastname: Iwasaki
authors.forename: M
authors.initials: M
authors.lastname: Hosaka
mesh_terms: D000328:Adult; D004542:Ejaculation / Q000187:drug effects*; D004573:Electrolytes / Q000008:administration & dosage* / Q000494:pharmacology; D005260:Female; D006801:Humans; D007317:Insemination, Artificial, Homologous; D008297:Male; D009994:Osmolar Concentration; D011247:Pregnancy / Q000502:physiology*; D011256:Pregnancy Outcome; D012996:Solutions; D013048:Specimen Handling / Q000379:methods*; D013081:Sperm Motility / Q000502:physiology; D013094:Spermatozoa / Q000502:physiology*; D001743:Urinary Bladder
chemical_list: D004573:Electrolytes; D012996:Solutions