title: Inactivation of enveloped viruses by singlet oxygen thermally generated from a polymeric naphthalene derivative.
abstract: Inactivation of viruses can be induced by singlet oxygen generating agents. The water-insoluble polymeric compound PVNE (poly (1,4-dimethyl-6-vinylnaphthalene-1,4-endoperoxide)) is used as a storage for reactive oxygen and is able to produce thermally generated 1O2 in a dark-reaction. Enveloped viruses from two different families, Semliki Forest virus (SFV, Togaviridae) and vesicular stomatitis virus (VSV, Rhabdoviridae) showed a loss of infectivity of up to 8 log10/ml (TCID50) when incubated at 37 degrees C with PVNE in buffered solutions. PVNE produces singlet oxygen by thermal decomposition without irradiation. Such chemically generated oxygen excludes reactions involving radicals (type I photoreactions), a problem often encountered in photodynamic processes utilizing dyes as sensitizers. In addition, the water insolubility of the oxygen-carrier allows an easy removal and recycling from aqueous solutions. Therefore, it may prove useful in the inactivation of viruses in biological systems and may be a helpful tool in studies concerning the inactivation mechanism by 1O2.
authors.affiliation: Department of Chemistry and Biochemistry, University of Bern, Switzerland.
authors.forename: F
authors.initials: F
authors.lastname: Käsermann
authors.forename: C
authors.initials: C
authors.lastname: Kempf
mesh_terms: D000330:Aedes; D000818:Animals; D000998:Antiviral Agents / Q000138:chemical synthesis / Q000494:pharmacology*; D002460:Cell Line; D007700:Kinetics; D015394:Molecular Structure; D009281:Naphthalenes / Q000138:chemical synthesis / Q000494:pharmacology*; D010100:Oxygen; D011108:Polymers / Q000138:chemical synthesis / Q000494:pharmacology*; D011145:Polyvinyls / Q000138:chemical synthesis / Q000494:pharmacology*; D012672:Semliki forest virus / Q000187:drug effects*; D026082:Singlet Oxygen; D014721:Vesicular stomatitis Indiana virus / Q000187:drug effects*
chemical_list: D000998:Antiviral Agents; D009281:Naphthalenes; D011108:Polymers; D011145:Polyvinyls; C113032:poly(1,4-dimethyl-6-vinylnaphthalene-1,4-endoperoxide); D026082:Singlet Oxygen; D010100:Oxygen