Target range of zwittermicin A, an aminopolyol antibiotic from Bacillus cereus.
Zwittermicin A is a novel antibiotic produced by Bacillus cereus UW85, which suppresses certain plant diseases in the laboratory and in the field. We developed a rapid method for large-scale purification of zwittermicin A and then studied the in vitro activity of zwittermicin A against bacteria, fungi, and protists. Zwittermicin A was highly active against the Oomycetes and their relatives, the algal protists, and had moderate activity against diverse Gram-negative bacteria and certain Gram-positive bacteria as well as against a wide range of plant pathogenic fungi. Zwittermicin A was more active against bacteria and fungi at pH 7-8 than at pH 5-6. When zwittermicin A was combined with kanosamine, another antibiotic produced by B. cereus, the two acted synergistically against Escherichia coli and additively against Phytophthora medicaginis, an Oomycete. The results indicate that there are diverse potential applications of this new class of antibiotic.
Silo-Suh
L A
LA
Department of Bacteriology, University of Wisconsin-Madison, Madison, WI 53706, USA.
Stabb
E V
EV
Raffel
S J
SJ
Handelsman
J
J
D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D001409:Bacillus cereus / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid; D004359:Drug Therapy, Combination / Q000494:pharmacology; D005658:Fungi / Q000187:drug effects; D005944:Glucosamine / Q000494:pharmacology; D006090:Gram-Negative Bacteria / Q000187:drug effects; D006094:Gram-Positive Bacteria / Q000187:drug effects; D006863:Hydrogen-Ion Concentration; D008826:Microbial Sensitivity Tests; D009868:Oomycetes / Q000187:drug effects; D010455:Peptides; D013045:Species Specificity
D000900:Anti-Bacterial Agents; D010455:Peptides; C088290:zwittermicin A; C014968:kanosamine; D005944:Glucosamine