Activation of latent transgenes in Arabidopsis using a hybrid transcription factor.
A hybrid transcription factor comprising a fusion of the DNA-binding domain of Saccharomyces cerevisiae GAL4 and the transcription activation domain of maize C1 was expressed in stably transformed Arabidopsis. Additional transgenic lines were created containing test genes controlled by a synthetic promoter consisting of concatemeric copies of the cis-acting site recognized by GAL4 (UASG) fused to a minimal promoter. The GAL4/C1 effector line was crossed to two lines containing a synthetic promoter/GUS fusion. Both histochemical staining and GUS activity assays indicate strong activation of GUS expression was achieved only after crossing. The GAL4/C1 effector line was also crossed to 15 lines containing a synthetic promoter/antisense adenylosuccinate synthetase gene. Severely retarded growth, and in some cases lethality, was observed in 40% of the F1 lines. This system of activation by crossing is generally useful for activating expression of test transgenes.
Guyer
D
D
Novartis Agricultural Biotechnology Research, Research Triangle Park, North Carolina 27709, USA.
Tuttle
A
A
Rouse
S
S
Volrath
S
S
Johnson
M
M
Potter
S
S
Görlach
J
J
Goff
S
S
Crossland
L
L
Ward
E
E
D017360:Arabidopsis / Q000235:genetics*; D003433:Crosses, Genetic; D004268:DNA-Binding Proteins; D005656:Fungal Proteins / Q000032:analysis / Q000235:genetics; D005786:Gene Expression Regulation / Q000235:genetics*; D030821:Plants, Genetically Modified / Q000235:genetics; D011993:Recombinant Fusion Proteins / Q000502:physiology*; D012231:Rhizobium / Q000235:genetics; D012441:Saccharomyces cerevisiae / Q000235:genetics; D029701:Saccharomyces cerevisiae Proteins; D014157:Transcription Factors / Q000235:genetics*; D014170:Transformation, Genetic; D019076:Transgenes / Q000235:genetics*
D004268:DNA-Binding Proteins; D005656:Fungal Proteins; C052130:GAL4 protein, S cerevisiae; D011993:Recombinant Fusion Proteins; D029701:Saccharomyces cerevisiae Proteins; D014157:Transcription Factors