title: Cloning and characterization of the mouse and rat type II arginase genes.
abstract: Two forms of arginase, both catalyzing the hydrolysis of arginine to ornithine and urea, are found in animals ranging from amphibians to mammals. In humans, inherited deficiency of hepatic or type I arginase results in hyperargininemia, a syndrome characterized by periodic episodes of hyperammonemia, spasticity, and neurological deterioration. In these patients, a second extrahepatic or type II arginase activity is significantly increased, an induction that may partially compensate for the lack of AI activity and apparently mitigates some of the clinical effects of the condition. Cloning and characterization of the human AII cDNA was recently accomplished. The cloning, sequencing, and partial characterization of the mouse and rat AII cDNAs are reported herein. The DNA sequences predicted polypeptides of 354 amino acids, including a N-terminal mitochondrial import signal. Sequence homology to the human type II arginase, arginase activity data, and immunoprecipitation with an anti-AII antibody confirm the identity of these cloned genes as rodent extrahepatic type II arginases.
authors.affiliation: Department of Pathology, UCLA School of Medicine, California 90095, USA.
authors.forename: R K
authors.initials: RK
authors.lastname: Iyer
authors.forename: J M
authors.initials: JM
authors.lastname: Bando
authors.forename: C P
authors.initials: CP
authors.lastname: Jenkinson
authors.forename: J G
authors.initials: JG
authors.lastname: Vockley
authors.forename: P S
authors.initials: PS
authors.lastname: Kim
authors.forename: R M
authors.initials: RM
authors.lastname: Kern
authors.forename: S D
authors.initials: SD
authors.lastname: Cederbaum
authors.forename: W W
authors.initials: WW
authors.lastname: Grody
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001119:Arginase / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D003001:Cloning, Molecular; D018076:DNA, Complementary; D004926:Escherichia coli / Q000378:metabolism; D005260:Female; D006801:Humans; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D008969:Molecular Sequence Data; D010802:Phylogeny; D011233:Precipitin Tests; D051381:Rats; D017207:Rats, Sprague-Dawley; D017386:Sequence Homology, Amino Acid; D014508:Urea / Q000378:metabolism
chemical_list: D018076:DNA, Complementary; D014508:Urea; C113162:Arg2 protein, rat; D001119:Arginase