Defective human T-cell lymphotropic virus type I (HTLV-I) provirus in 10 Chilean seronegative patients with tropical spastic paraparesis or HTLV-I-associated myelopathy.
We studied the presence of tax and ltr genes from human T-cell lymphotropic virus type I (HTLV-I) provirus in the peripheral blood mononuclear cells from 15 seronegative patients with tropical spastic paraparesis or HTLV-I-associated myelopathy by PCR. Only a region of the tax gene from 10 patients was amplified. The nucleotide homologies of six Chilean isolates to the ATK-1 clone ranged between 98.7 and 99.4%.
Ramirez
E
E
Department of Health Laboratories, Public Health Institute of Chile, Santiago. eramirez@ispch.cl
Cartier
L
L
Rios
M
M
Fernandez
J
J
D000328:Adult; D000368:Aged; D001483:Base Sequence; D015153:Blotting, Western; D002677:Chile; D003673:Defective Viruses / Q000235:genetics / Q000302:isolation & purification*; D005260:Female; D019084:Fluorescent Antibody Technique, Indirect; D016355:Genes, pX; D015481:HTLV-I Antibodies / Q000097:blood; D015490:HTLV-I Infections / Q000276:immunology / Q000821:virology*; D015368:Human T-lymphotropic virus 1 / Q000235:genetics / Q000302:isolation & purification*; D006801:Humans; D008297:Male; D008875:Middle Aged; D008969:Molecular Sequence Data; D015493:Paraparesis, Tropical Spastic / Q000276:immunology / Q000821:virology*; D016133:Polymerase Chain Reaction; D011533:Proviruses / Q000235:genetics / Q000302:isolation & purification*
D015481:HTLV-I Antibodies