title: Internalization with high targeting potential of mouse monoclonal antibody ONS-M21 recognizing human malignant glioma antigen.
abstract: In order to evaluate the targeting potential of mouse monoclonal antibody ONS-M21 recognizing a human astrocytoma- and medulloblastoma-associated antigen, the internalization ability of this antibody and the selective cytotoxicity in the toxin-conjugated form were examined. Internalization assay with 125I-labeled ONS-M21 showed that about 20% of the total radioactivities was detected in the cellular fraction of human medulloblastoma cell line ONS-76 cells and that the reaction reached a plateau level in 30 min. To examine the selective delivery capacity of a high molecular substance in place of 125I, an immunotoxin was prepared with ricin A chain and ONS-M21 via disulfide bonds. A cytotoxic effect against ONS-76 cells was found with [3H]thymidine incorporation assay using the immunotoxin, but not against antigen-negative HuH-7 and SW480 cells. These results suggest that ONS-M21 could effectively deliver toxins, chemotherapeutic agents or radionuclei to malignant glioma specifically.
authors.affiliation: Department of Neurosurgery, Osaka University Medical School, Suita City, Japan.
authors.forename: K
authors.initials: K
authors.lastname: Shimizu
authors.forename: K C
authors.initials: KC
authors.lastname: Park
authors.forename: K
authors.initials: K
authors.lastname: Tamura
authors.forename: H
authors.initials: H
authors.lastname: Kishima
authors.forename: H
authors.initials: H
authors.lastname: Kawata
authors.forename: Y
authors.initials: Y
authors.lastname: Yoshimura
authors.forename: Y
authors.initials: Y
authors.lastname: Sekimori
authors.forename: Y
authors.initials: Y
authors.lastname: Miyao
authors.forename: T
authors.initials: T
authors.lastname: Hayakawa
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal; D000951:Antigens, Neoplasm / Q000032:analysis*; D001254:Astrocytoma / Q000276:immunology; D002470:Cell Survival; D005069:Evaluation Studies as Topic; D005910:Glioma / Q000276:immunology*; D006801:Humans; D000922:Immunotoxins; D008527:Medulloblastoma / Q000276:immunology; D051379:Mice; D012276:Ricin / Q000494:pharmacology; D014407:Tumor Cells, Cultured
chemical_list: D000911:Antibodies, Monoclonal; D000951:Antigens, Neoplasm; D000922:Immunotoxins; D012276:Ricin