title: Behaviour and enterotoxin production by Staphylococcus aureus during the manufacture and ripening of raw goats' milk lactic cheeses.
abstract: To study the possible presence of staphylococcal enterotoxin A in raw goats' milk lactic cheese, milk was inoculated with an enterotoxigenic Staphylococcus aureus strain to a final concentration of 4, 5 and 6 log(cfu/ml). Cheese was prepared following industrial specifications and ripened for 42 d. Detection of the enterotoxins was by the Vidas Staph enterotoxin test (BioMérieux) and by an indirect double-sandwich ELISA technique using anti-enterotoxin monoclonal antibodies. Staphylococcal counts declined markedly after draining, and by the end of ripening they had disappeared from some cheeses. In contrast, aerobic mesophilic organisms grew well. The level of staphylococcal enterotoxin A recovered varied from 1 to 2.5 ng/g cheese made with an initial population of 10(5) or 10(6) cfu/ml. Only traces of enterotoxin A (0.5 ng/g) were detected in cheeses made with the lowest Staph. aureus inoculum used in this study. Enterotoxin A was also detected in cheeses from which Staph. aureus had disappeared.
authors.affiliation: Unité de Microbiologie Alimentaire et Prévisionnelle, Ecole Nationale Vétérinaire de Lyon, Marcy l'Etoile, France.
authors.forename: C
authors.initials: C
authors.lastname: Vernozy-Rozand
authors.forename: A
authors.initials: A
authors.lastname: Meyrand
authors.forename: C
authors.initials: C
authors.lastname: Mazuy
authors.forename: M L
authors.initials: ML
authors.lastname: Delignette-Muller
authors.forename: G
authors.initials: G
authors.lastname: Jaubert
authors.forename: G
authors.initials: G
authors.lastname: Perrin
authors.forename: C
authors.initials: C
authors.lastname: Lapeyre
authors.forename: Y
authors.initials: Y
authors.lastname: Richard
mesh_terms: D000818:Animals; D002611:Cheese / Q000032:analysis*; D004768:Enterotoxins / Q000032:analysis / Q000096:biosynthesis*; D005260:Female; D005511:Food Handling; D006041:Goats; D008892:Milk / Q000737:chemistry / Q000382:microbiology*; D013211:Staphylococcus aureus / Q000302:isolation & purification / Q000502:physiology*; D018089:Superantigens / Q000032:analysis / Q000096:biosynthesis
chemical_list: D004768:Enterotoxins; D018089:Superantigens; C026066:enterotoxin A, Staphylococcal