[Effect of n-palmitoylethanolamine on energy-dependent transport of Ca2+ in intracellular structures of myometrium and their phospholipid composition].
N-palmitoylethanolamine (NPE) was studied for its effect on the systems of energy-dependent transport of Ca2+ in the intracell structures (mitochondria, endoplasmic reticulum) of permeabilized cells of smooth muscles of the rat uterus as well as on the lipid composition of myocytes. NPE in concentration of 10(-5) M partially (by 30-50%) inhibited energy-dependent accumulation of Ca2+ in endoplasmic reticulum and mitochondria of myometrium cells permeabilized by means of treatment of myocytes suspension by digitonin (0.1 mg/ml). NPE modifies the lipid composition of permeabilized myocytes when determining the increase of the amount of inorganic phosphorus of total phospholipids by 57.3% at the expense of considerable accumulation of phosphatidylcholine, phosphatidylethanolamine and sphingomyelin. It is supposed that the effect of NPE on the systems of energy-dependent transport of Ca2+ is achieved through the modification of phospholipid composition of a cell, while modulating effect of NPE on the active transmembrane transfer of Ca2+ in the intracellular structure can be an important link in the general mechanism of the effect of this compound on Ca2+ metabolism in myometrium and on Ca(2+)-depended control of the contracting function of the uterus.
Gulaia
N M
NM
A.V. Palladin Institute of Biochemistry, National Academy of Sciences of Ukraine, Kyiv.
Babich
L G
LG
Shlykov
S G
SG
Margitich
V M
VM
Govseeva
N N
NN
Klimashevskiĭ
V M
VM
Kosterin
S A
SA
D000577:Amides; D000818:Animals; D001692:Biological Transport / Q000187:drug effects; D002118:Calcium / Q000493:pharmacokinetics*; D063388:Endocannabinoids; D004721:Endoplasmic Reticulum / Q000187:drug effects / Q000378:metabolism; D004734:Energy Metabolism / Q000187:drug effects*; D004983:Ethanolamines; D005260:Female; D008928:Mitochondria / Q000187:drug effects / Q000378:metabolism; D009215:Myometrium / Q000187:drug effects* / Q000378:metabolism / Q000648:ultrastructure; D010169:Palmitic Acids / Q000494:pharmacology*; D010743:Phospholipids / Q000378:metabolism*; D051381:Rats
D000577:Amides; D063388:Endocannabinoids; D004983:Ethanolamines; D010169:Palmitic Acids; D010743:Phospholipids; C005958:palmidrol; D002118:Calcium
Vliianie n-pal'mitoilétanolamina na énergozavisimyĭ transport Ca2+ vo vnutrikletochnykh strukturakh miometriia i ikh fosfolipidnyĭ sostav.