Characterization of MGC2, a Mycoplasma gallisepticum cytadhesin with homology to the Mycoplasma pneumoniae 30-kilodalton protein P30 and Mycoplasma genitalium P32.
A second cytadhesin-like protein, MGC2, was identified in the avian respiratory pathogen Mycoplasma gallisepticum. The 912-nucleotide mgc2 gene encodes a 32.6-kDa protein with 40.9 and 31.4% identity with the M. pneumoniae P30 and M. genitalium P32 cytadhesins, respectively. Functional studies with reverse transcription-PCR, immunoblotting, double-sided immunogold labeling, and attachment inhibition assays demonstrated homology to the human mycoplasmal P30 and P32 cytadhesins. These findings suggest that there is a family of cytadhesin genes conserved among pathogenic mycoplasmas infecting widely divergent hosts.
Hnatow
L L
LL
Department of Animal and Food Sciences, College of Agriculture and Natural Resources, University of Delaware, Newark, Delaware 19717-1303, USA.
Keeler
C L
CL
Tessmer
L L
LL
Czymmek
K
K
Dohms
J E
JE
D018829:Adhesins, Bacterial / Q000032:analysis / Q000737:chemistry / Q000235:genetics*; D000595:Amino Acid Sequence; D000818:Animals; D002642:Chick Embryo; D003001:Cloning, Molecular; D004269:DNA, Bacterial / Q000737:chemistry; D006801:Humans; D007106:Immune Sera / Q000276:immunology; D008969:Molecular Sequence Data; D008970:Molecular Weight; D016297:Mutagenesis, Site-Directed; D009174:Mycoplasma / Q000737:chemistry* / Q000235:genetics / Q000502:physiology; D009177:Mycoplasma pneumoniae / Q000737:chemistry*; D009876:Operon; D016133:Polymerase Chain Reaction; D011817:Rabbits; D017385:Sequence Homology; D014158:Transcription, Genetic
D018829:Adhesins, Bacterial; D004269:DNA, Bacterial; D007106:Immune Sera