title: Effects of alpha-tocopherol on metmyoglobin formation and reduction in beef from cattle fed soybean or cottonseed meal diets.
abstract: Hereford-Angus crossbred heifers were fed a cottonseed meal-based diet containing gossypol (14 mg free gossypol x kg body wt(-1) x d(-1); CSM), a soybean meal-based diet (SBM), or alpha-tocopherol-supplemented diets (4,036 IU vitamin E x heifer(-1) x d(-1) for 90 d; CSM+E and SBM+E). The effects of diet on color stability and aerobic metmyoglobin reducing ability of beef longissimus lumborum (LL) and psoas major (PM) were evaluated. The CSM containing gossypol did not affect alpha-tocopherol concentration, a* value, or hue angle value of beef muscles obtained from control or vitamin E-supplemented cattle compared to their SBM counterparts. Vitamin E supplementation increased endogenous alpha-tocopherol concentrations and color stability in LL and PM muscles compared with controls from either diet (P < .05). In the aerobic metmyoglobin reducing ability study, LL and PM muscles were stored in 1% O2:99% N2 (a pigment-oxidizing atmosphere) for 48 h and subsequently stored aerobically for an additional 48 h. Within the LL, alpha-tocopherol supplementation delayed metmyoglobin formation in LL exposed to 1% O2 (P < .05). Within the PM, no differences in metmyoglobin formation were found between controls and vitamin E treatments in SBM or CSM diets. Relative aerobic metmyoglobin reduction was the same (P > .05) in LL and PM muscles within SBM or CSM diets for control and vitamin E treatments. Alpha-tocopherol did not seem to affect metmyoglobin aerobic reducing ability in LL and PM muscles.
authors.affiliation: Department of Animal Science, University of Connecticut, Storrs 06269, USA. KCHAN@ansc1.cag.uconn.edu
authors.forename: W K
authors.initials: WK
authors.lastname: Chan
authors.forename: C
authors.initials: C
authors.lastname: Faustman
authors.forename: J
authors.initials: J
authors.lastname: Velasquez-Pereira
authors.forename: L R
authors.initials: LR
authors.lastname: McDowell
authors.forename: T R
authors.initials: TR
authors.lastname: Batra
mesh_terms: D000818:Animals; D002417:Cattle / Q000378:metabolism*; D003369:Cottonseed Oil; D004032:Diet / Q000662:veterinary*; D005260:Female; D006072:Gossypol / Q000008:administration & dosage / Q000494:pharmacology; D008460:Meat / Q000592:standards; D008786:Metmyoglobin / Q000187:drug effects / Q000378:metabolism*; D018482:Muscle, Skeletal / Q000737:chemistry / Q000187:drug effects / Q000378:metabolism*; D010084:Oxidation-Reduction; D010858:Pigmentation / Q000187:drug effects; D016658:Psoas Muscles / Q000737:chemistry / Q000187:drug effects / Q000378:metabolism; D013025:Glycine max; D014810:Vitamin E / Q000008:administration & dosage / Q000032:analysis / Q000494:pharmacology*
chemical_list: D003369:Cottonseed Oil; D008786:Metmyoglobin; D014810:Vitamin E; D006072:Gossypol