Production of human lactoferrin in transgenic tobacco plants.
Production and characterization of human lactoferrin (hLf) in transgenic tobacco is reported. We have engineered two constructs containing either the native signal peptide from human lactoferrin or the signal peptide from sweet potato sporamin fused to human lactoferrin encoding cDNA. N-terminal sequences of rhLf purified from tobacco were identical to Lf from human milk for both constructs. The tobacco rhLf presents a molecular mass closely identical to native protein. Overall sugar composition shows the presence of plant specific xylose while sialic acid is absent. Binding parameters of the recombinant molecule to both Jurkat lymphoblastic T-cells or HT29-18-C1 enterocytes are similar to those of human lactoferrin isolated from milk.
Salmon
V
V
Laboratoire de Chimie Biologique, Centre National de la Recherche Scientifique No. 111, Université des Sciences et Technologies de Lille, Villeneuve d'Ascq, France.
Legrand
D
D
Slomianny
M C
MC
el Yazidi
I
I
Spik
G
G
Gruber
V
V
Bournat
P
P
Olagnier
B
B
Mison
D
D
Theisen
M
M
Mérot
B
B
D000595:Amino Acid Sequence; D001483:Base Sequence; D002241:Carbohydrates / Q000032:analysis; D017931:DNA Primers; D018076:DNA, Complementary; D006031:Glycosylation; D006801:Humans; D007781:Lactoferrin / Q000737:chemistry / Q000235:genetics* / Q000302:isolation & purification; D008969:Molecular Sequence Data; D008970:Molecular Weight; D030821:Plants, Genetically Modified; D010947:Plants, Toxic; D011485:Protein Binding; D011993:Recombinant Fusion Proteins / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D014026:Nicotiana / Q000235:genetics*
D002241:Carbohydrates; D017931:DNA Primers; D018076:DNA, Complementary; D011993:Recombinant Fusion Proteins; D007781:Lactoferrin