[Effect of cryopreservation on the morphology of preimplantation embryos of the ISIAH rat line].
Micronuclei of preimplantation embryos of the hypertensive rat strain with inherited spontaneously induced arterial hypertension (ISIAH) are studied. Also studied are the formation of micronuclei and other morphological changes in these embryos after freezing, cryoconservation, and consequent transplantation to Wistar recipient rats. Analysis of semithin sections demonstrates that the above procedures lead to the formation of micronuclei in most embryos, while the shape of nuclei changes in only some embryos. There is a tendency towards perinuclear location of organelles and inclusions and to the partial disappearance of polarity in the majority of blastomeres. We believe that the increase in the number of micronuclei can be associated with a certain selection of embryos during cryoconservation and transplantation.
Amstislavskiĭ
S Ia
SIa
Institute of Cytology and Genetics, Siberian Division, Russian Academy of Sciences, Novosibirsk, Russia.
Kachanova
I Iu
IIu
Kizilova
E A
EA
Maksimovskiĭ
L F
LF
Markel'
A L
AL
Iakobson
G S
GS
D000818:Animals; D001755:Blastocyst / Q000648:ultrastructure*; D001757:Blastomeres / Q000648:ultrastructure; D015925:Cryopreservation; D006973:Hypertension / Q000235:genetics*; D048629:Micronuclei, Chromosome-Defective / Q000637:transplantation*; D051381:Rats; D011919:Rats, Inbred Strains; D017208:Rats, Wistar
Vliianie kriokonservatsii na mofologiiu doimplantatsionnykh zarodysheĭ krys linii NISAG