[Methods of measuring telomere length and telomerase activity--practice and problems].
The development of a highly sensitive method for detection of telomerase activity, telomeric repeat amplification protocol (TRAP), has provided knowledge on telomerase activity in normal and cancer tissues. Subsequent several modifications have been achieved, including an introduction of the internal standard and hybridization protection technique that leads to simplicity and improvement of reproducibility and linearity of this method, and application of TRAP to in situ analysis to identify the cells responsible for telomerase activity. As for measurement of telomere length, fluorescence in situ hybridization technique appeared to give an information of telomere length on an individual chromosome in contrast to analysis of terminal restriction fragment, a conventional method which can express mean telomere length of all chromosomes. Further methodological improvement in this field is ongoing and showing a new sight on cell mortality and immortality.
Saito
Y
Y
First Department of Surgery, Niigata University School of Medicine.
Suda
T
T
Hatakeyama
K
K
D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D008722:Methods; D012091:Repetitive Sequences, Nucleic Acid; D019098:Telomerase / Q000032:analysis*; D016615:Telomere / Q000235:genetics*
D019098:Telomerase