Adeno-associated virus vector mediated transduction of primary normal human breast epithelial cells.
Cultured human breast epithelial cells from reduction mammoplasty specimens were transduced using an adeno-associated virus vector encoding the marker gene E. coli -galactosidase. Subconfluent, growing, breast epithelial cells were more easily transduced than confluent, quiescent, cells. Transduction of non-dividing confluent cells could be greatly increased by ultraviolet light-induced DNA damage or by prior exposure to the DNA synthesis inhibitor hydroxyurea. The effects of ultraviolet light and hydroxyurea on transduction were additive when these agents were applied together.
Yang
J
J
Cancer Research Laboratory, 491 LSA, University of California, Berkeley, CA 94720, USA.
Hirokawa
Y
Y
Dougherty
C
C
Colosi
P
P
Yang
J H
JH
Nandi
S
S
D001940:Breast / Q000166:cytology / Q000821:virology*; D002478:Cells, Cultured; D004249:DNA Damage; D000229:Dependovirus / Q000235:genetics*; D004847:Epithelial Cells / Q000821:virology*; D005260:Female; D018014:Gene Transfer Techniques; D005822:Genetic Vectors; D006801:Humans; D006918:Hydroxyurea / Q000494:pharmacology; D007763:Lac Operon; D016462:Mammaplasty; D019384:Nucleic Acid Synthesis Inhibitors / Q000494:pharmacology; D014161:Transduction, Genetic / Q000528:radiation effects*; D014466:Ultraviolet Rays
D019384:Nucleic Acid Synthesis Inhibitors; D006918:Hydroxyurea