Effects of transferrin on the modulation of cytokine production on mouse spleen cells.
In order to investigate the the effects of transferrin(Tf) on the production of cytokines, mouse spleen cells were treated with various concentrations of apo- and holo-Tf, and then the production of IL-6, IFN gamma and the expression of mRNA for TNF alpha was determined. The distribution of Tf, macrophages and T cells in the mouse mammary glands was also examined. IL-6 and IFN gamma producing capabilities of the unstimulated spleen cells in the presence of apo and holo-Tf were increased in a dose dependent manner, while the cells stimulated with anti-CD3 had no significant effects on production in thd presence of graded concentrations of Tf. The relative abundance of TNF alpha mRNA was significantly affected by the concentration of TF. During early involution almost all of the secretory epithelial cells and the secretion in the alveoli showed a very strong positive reaction to transferrin antibody, and macrophages and T cells were distributed in the lumen, alveolar epithelial layer and connective tissue area. These findings suggest that the upregulated patterns of these cytokines and distribution of immune cells may play a beneficial role in the augmentation of host's defense mechanisms during involution.
Ryu
S Y
SY
Department of Veterinary Medicine, College of Veterinary Medicine, Chungnam National University, Taejon, Korea.
Jeong
K S
KS
Park
S J
SJ
Mheen
B G
BG
Son
H Y
HY
Yoon
W K
WK
Cho
S W
SW
Park
B K
BK
Kim
S H
SH
D000818:Animals; D002478:Cells, Cultured; D016207:Cytokines / Q000096:biosynthesis* / Q000187:drug effects; D004306:Dose-Response Relationship, Immunologic; D005260:Female; D007150:Immunohistochemistry; D008213:Lymphocyte Activation / Q000187:drug effects; D008297:Male; D008321:Mammary Glands, Animal / Q000737:chemistry; D051379:Mice; D008809:Mice, Inbred C3H; D012333:RNA, Messenger / Q000096:biosynthesis / Q000187:drug effects; D013154:Spleen / Q000187:drug effects* / Q000276:immunology / Q000378:metabolism*; D014168:Transferrin / Q000494:pharmacology*; D014409:Tumor Necrosis Factor-alpha / Q000096:biosynthesis / Q000235:genetics
D016207:Cytokines; D012333:RNA, Messenger; D014168:Transferrin; D014409:Tumor Necrosis Factor-alpha