Chemiluminescent derivatization of adenyl compounds with glyoxal derivatives in the presence of heteropoly acids and its application to the simple and sensitive determination of DNA.
A chemiluminescence (CL) determination of adenyl compounds is described. CL derivatization of adenyl compounds with methylglyoxal dimethyl acetal was performed in the presence of tungstosilicic acid and propan-2-ol. CL from adenyl compounds was produced by hydrogen peroxide and L-cysteine ethyl ester in DMF and water. The proposed method is highly sensitive and specific to compounds containing adenine. Adenine was determined in the range 1.0 x 10(-3)-5.0 x 10(-8) M with the detection limit of 3.0 x 10(-8) M (150 fmol per assay). The method was applied to the determination of DNA and detection limits of a few nanograms of DNA achieved.
Kuroda
N
N
School of Pharmaceutical Sciences, Nagasaki University, Japan.
Nakashima
K
K
Akiyama
S
S
Sato
N
N
Imi
N
N
Shirakawa
K
K
Uemura
A
A
D000096:Acetone / Q000031:analogs & derivatives / Q000737:chemistry; D000148:Acids, Noncarboxylic / Q000737:chemistry*; D000225:Adenine / Q000031:analogs & derivatives* / Q000737:chemistry*; D004279:DNA, Viral / Q000032:analysis*; D006037:Glyoxal / Q000031:analogs & derivatives* / Q000737:chemistry*; D006515:Hepatitis B virus / Q000235:genetics; D006801:Humans; D008163:Luminescent Measurements; D008982:Molybdenum / Q000737:chemistry; D009942:Organometallic Compounds / Q000737:chemistry*; D010756:Phosphoric Acids / Q000737:chemistry; D016133:Polymerase Chain Reaction; D012680:Sensitivity and Specificity; D017640:Silicates / Q000737:chemistry; D017655:Silicon Compounds / Q000737:chemistry; D017973:Tungsten Compounds / Q000737:chemistry
D000148:Acids, Noncarboxylic; D004279:DNA, Viral; D009942:Organometallic Compounds; D010756:Phosphoric Acids; D017640:Silicates; D017655:Silicon Compounds; D017973:Tungsten Compounds; C113465:methylglyoxal dimethyl acetal; C013250:silicomolybdate; D000096:Acetone; D006037:Glyoxal; D008982:Molybdenum; C021746:silicotungstic acid; D000225:Adenine; C003125:phosphomolybdic acid