title: [Isolation and specificity of novel restriction endonucleases Bsp40091 and AsiI, isoschizomers of BamHI].
abstract: New type II restriction endonucleases AsiI and Bsp40091 are detected in Azotobacter species N55 and Bacillus species 4009, respectively. Purified preparations of the restriction enzymes free from interfering nucleases and phosphatases were obtained by column chromatography on phosphocellulose and heparin-sepharose (Asil) and phosphocellulose and DEAE-cellulose (Bsp40091). The yield of purified AsiI and Bsp40091 was 16 x 10(3) and 8 x 10(3) units per g of wet cells, respectively. The above restriction endonucleases recognize the 5'-G decreases GATCC-3' sequence on double-stranded DNA and cleave it as shown, thus being true isoschizomers of BamHI restriction endonuclease.
authors.forename: S V
authors.initials: SV
authors.lastname: Korolev
authors.forename: N N
authors.initials: NN
authors.lastname: Sokolov
authors.forename: M
authors.initials: M
authors.lastname: Rina
authors.forename: M A
authors.initials: MA
authors.lastname: El'darov
authors.forename: N M
authors.initials: NM
authors.lastname: Omel'ianiuk
authors.forename: M
authors.initials: M
authors.lastname: Markaki
authors.forename: K G
authors.initials: KG
authors.lastname: Skriabin
authors.forename: V
authors.initials: V
authors.lastname: Bouriotis
mesh_terms: D001395:Azotobacter / Q000201:enzymology*; D001407:Bacillus / Q000201:enzymology*; D015245:Deoxyribonuclease BamHI / Q000302:isolation & purification*; D013045:Species Specificity; D013379:Substrate Specificity
chemical_list: D015245:Deoxyribonuclease BamHI
vernacular_title: Vydelenie i opredelenie spetsifichnosti novykh éndonukleaz restriktsii Bsp40091 i AsiI izoshizomerov BamHI.