Proteoglycans in human coronary arteriosclerotic lesions.
Proteoglycans in human coronary arteries were characterized immunohistochemically, using specific monoclonal antibodies to distinct proteoglycan types. In addition, apoB, macrophage, and arterial smooth muscle cell alpha-actin markers were localized. The expression of chondroitin sulfate proteoglycans and apoB was observed in healthy areas (operationally defined by morphology) as well as in lesions in the intima, but with greater expression in the atheromatous lesions. In healthy intima heparan sulfate proteoglycan was cell associated, but in lesions it was found also in the extracellular space. A dermatan sulfate proteoglycan of decorin type was not observed in the healthy intima but was observed in the intima with adaptive thickening especially in zones with reduced staining for smooth muscle cell alpha-actin. In atheroma (fibrous plaque with necrotic core) decorin along with alpha-actin and macrophage marker stained brightly in the extracellular regions in fibrous cap (actively progressing lesion) but was sparse in fibrous base (quiescent lesion). The observations suggest that decorin along with extracellular alpha-actin and macrophage marker may be useful for differentiating lesions that tend to progress with disease.
Radhakrishnamurthy
B
B
Tulane Center for Cardiovascular Health, Tulane School of Public Health and Tropical Medicines, New Orleans, Louisiana, USA.
Tracy
R E
RE
Dalferes
E R
ER
Berenson
G S
GS
D000199:Actins / Q000378:metabolism; D000328:Adult; D001055:Apolipoproteins B / Q000378:metabolism; D015415:Biomarkers; D011508:Chondroitin Sulfate Proteoglycans / Q000378:metabolism; D003324:Coronary Artery Disease / Q000378:metabolism* / Q000473:pathology; D058575:Decorin; D003871:Dermatan Sulfate / Q000378:metabolism; D016326:Extracellular Matrix Proteins; D005110:Extracellular Space / Q000378:metabolism; D005260:Female; D019812:Heparan Sulfate Proteoglycans / Q000378:metabolism; D006801:Humans; D007150:Immunohistochemistry; D008264:Macrophages / Q000378:metabolism / Q000473:pathology; D008297:Male; D008875:Middle Aged; D009131:Muscle, Smooth, Vascular / Q000378:metabolism / Q000473:pathology; D011509:Proteoglycans / Q000378:metabolism*
D000199:Actins; D001055:Apolipoproteins B; D015415:Biomarkers; D011508:Chondroitin Sulfate Proteoglycans; C546878:DCN protein, human; D058575:Decorin; D016326:Extracellular Matrix Proteins; D019812:Heparan Sulfate Proteoglycans; D011509:Proteoglycans; C032649:dermatan sulfate proteoglycan; D003871:Dermatan Sulfate