Ionizing radiation and hydrogen peroxide induced oxidative DNA base damage in two L5178Y cell lines.
Seven oxidized DNA bases were quantified, by gas GC/MS-SIM, in chromatin from gamma-rays and H2O2 treated mouse lymphoma L5178Y (LY) cells, inversely cross-sensitive to these agents. In H2O2 treated cells (2 mM, 1 h, 37 degrees C) we found more damage in LY-R cells than in LY-S cells. On the contrary, in gamma-rays (400 Gy) treated cells we found more damaged DNA bases in LY-S cells. The yield of damaged bases in control cells was similar in both cell lines, with the exception of 8OHAde and FapyGua that were found at a much higher level in LY-S cells. The yields of damaged bases were related to cellular sensitivity to damaging agent; this observation points to a relationship between DNA base damage induction, antioxidant defense system in the intracellular milieu and cell sensitivity.
Zastawny
T H
TH
Department of Clinical Biochemistry, University School of Medical Sciences, Bydgoszcz, Poland. tomekz@aci.amb.bydgoszcz.pl
Kruszewski
M
M
Olinski
R
R
D000818:Animals; D000975:Antioxidants / Q000378:metabolism; D004249:DNA Damage; D004273:DNA, Neoplasm / Q000187:drug effects / Q000378:metabolism / Q000528:radiation effects; D005609:Free Radicals / Q000378:metabolism; D006861:Hydrogen Peroxide / Q000633:toxicity*; D007940:Leukemia L5178 / Q000378:metabolism; D051379:Mice; D010084:Oxidation-Reduction; D011836:Radiation Tolerance; D017382:Reactive Oxygen Species / Q000378:metabolism; D014407:Tumor Cells, Cultured
D000975:Antioxidants; D004273:DNA, Neoplasm; D005609:Free Radicals; D017382:Reactive Oxygen Species; D006861:Hydrogen Peroxide