Effect of stobadine on carbon tetrachloride-induced erythrocyte membrane changes in rats.
Our previous study showed that stobadine is effective against ischemia/reperfusion-induced gastric mucosal injury. The present study examined the ability of stobadine to protect erythrocyte membrane against free radical injury after long-term carbon tetrachloride (CCl4) application. The erythrocyte membrane changes were established using colloid-osmotic hemolysis. The significant increase of colloid-osmotic hemolysis was found in animals treated with CCl4. CCl4 also increased formation of thiobarbituric acid-reactive substances (TBARs) and decreased thiol group content. Stobadine in both doses (10.0 and 20.0 mg.kg-1) protected erythrocyte membrane against CCl4-induced injury. The membrane lipid bilayer is the most affected part of the erythrocyte membrane. In presence of stobadine, CCl4-induced lipid peroxidation was partially or totally prevented whereas the level of total membrane thiols was increased. Based on these results, it can be concluded that protective effect of stobadine on CCl4-induced erythrocyte membrane changes should be related to its antioxidant properties.
Mojzis
J
J
Department of Pharmacology, Medical Faculty, P.J. Safarik University Kosice, Slovak Republic.
Nicák
A
A
Guzy
J
J
Kron
I
I
Mirossay
L
L
D000818:Animals; D000975:Antioxidants / Q000494:pharmacology*; D002243:Carbolines / Q000494:pharmacology*; D002251:Carbon Tetrachloride / Q000633:toxicity*; D004910:Erythrocyte Membrane / Q000187:drug effects* / Q000378:metabolism*; D005609:Free Radicals / Q000378:metabolism; D006461:Hemolysis; D066298:In Vitro Techniques; D008051:Lipid Bilayers / Q000378:metabolism; D015227:Lipid Peroxidation / Q000187:drug effects; D008297:Male; D051381:Rats; D017208:Rats, Wistar; D013438:Sulfhydryl Compounds / Q000378:metabolism; D017392:Thiobarbituric Acid Reactive Substances / Q000378:metabolism
D000975:Antioxidants; D002243:Carbolines; D005609:Free Radicals; D008051:Lipid Bilayers; D013438:Sulfhydryl Compounds; D017392:Thiobarbituric Acid Reactive Substances; D002251:Carbon Tetrachloride; C100106:dicarbine