title: Gum heteropolysaccharide and free reducing mono- and oligosaccharides of Anadenanthera colubrina.
abstract: The gum from Anadenanthera colubrina consists mainly of a complex high-arabinose heteropolysaccharide with a (1-->3)-linked beta-D-Galp main-chain and many different side-chains. These contain beta-D-Galp-[(1-->6)-beta-D-Galp]m-(1-->6)-, substituted in turn at O-3 by alpha-L-Araf-[(1-->3)-alpha-L-Araf-]0-2. Also present are (1) main-chain units substituted at O-4 and O-6 by alpha-L-Araf units, (2) side-chains of Rhap-(1-->4)-beta-D-GlcpA-(1-->6)-beta-Galp-groups, (3) alpha-L-Arap non-reducing end-units linked (1-->6) to D-Galp, and (4) beta-Araf and beta-Arap structures. For the first time, a plant gum exudate was found to contain in the natural state, reducing low M(r) carbohydrates. These were rhamnose (0.6%), arabinose (4.7%), mannose (0.1%), galactose (0.8%) and many oligosaccharides (0.6%; 11 with different RFs, with the majority containing arabinose). They were all mixtures with the exception of alpha-Rhap-(1-->4)-beta-D-GlcpA-(1-->6)-alpha beta-Gal and an incompletely identified hexasaccharide, probably having alpha-L-Araf-(1-->4)-beta-D-Galp- and -alpha-L-Araf-(1-->3)-beta-D-Galp- structures. The mono- and oligosaccharides do not appear to arise via in situ autohydrolysis of the gum.
authors.affiliation: Departamento de Bioquímica, Universidade Federal do Paraná, Curitiba-PR, Brazil.
authors.forename: C L
authors.initials: CL
authors.lastname: Delgobo
authors.forename: P A
authors.initials: PA
authors.lastname: Gorin
authors.forename: C
authors.initials: C
authors.lastname: Jones
authors.forename: M
authors.initials: M
authors.lastname: Iacomini
mesh_terms: D002236:Carbohydrate Conformation; D002240:Carbohydrate Sequence; D007887:Fabaceae / Q000737:chemistry*; D009682:Magnetic Resonance Spectroscopy; D008969:Molecular Sequence Data; D009005:Monosaccharides / Q000737:chemistry*; D009844:Oligosaccharides / Q000737:chemistry*; D010946:Plants, Medicinal; D011134:Polysaccharides / Q000737:chemistry*; D013237:Stereoisomerism
chemical_list: D009005:Monosaccharides; D009844:Oligosaccharides; D011134:Polysaccharides