Intrahepatic lymphocyte analyses and assessment of the effects of levamisole in murine hepatic metastasis model.
We used hepatic metastasis models to determine the mechanism and effect of levamisole. BALB/c mice and Colon 26 cells were used. Group I was injected with tumor cells through the portal vein. Group II was primed with tumor cells before tumor cells injecting. Group III was same as Group II, but treated with levamisole. Surface antigens of intrahepatic lymphocytes and spleen cells were determined by FACScan with Anti-CD3, anti-CD4, anti-CD8, anti-CD45, anti-NK1.1 and anti-F4/80. Nodules on the liver were greatest in Group I and fewest in Group III. Concerning intrahepatic lymphocytes, Group II, when compared with Group I, had increases of CD3+, CD4+ and CD8+ cells, and decreases of CD45+ and NK1.1+ cells. Group III when compared with Group II, showed increased CD8+ cells and decreased of NK1.1+ cells. Levamisole is considered to be effective in the prevention of liver metastasis and is suggeste to enhanced CD8+ cells.
Endo
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Department of Surgery, Tokyo Women's Medical College Daini Hospital, Tokyo, Japan.
Kinoshita
J
J
Yoshimatsu
K
K
Kato
H
H
Haga
S
S
Kajiwara
T
T
D000276:Adjuvants, Immunologic / Q000494:pharmacology*; D000818:Animals; D000954:Antigens, Surface / Q000032:analysis; D007978:Levamisole / Q000494:pharmacology*; D008113:Liver Neoplasms / Q000276:immunology / Q000517:prevention & control / Q000556:secondary*; D008214:Lymphocytes / Q000276:immunology*; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C
D000276:Adjuvants, Immunologic; D000954:Antigens, Surface; D007978:Levamisole