Non-enzymatic, template-directed ligation of 2'-5' oligoribonucleotides. Joining of a template and a ligator strand.
Decauridylate containing exclusively a 2'-5' phospho-diester bond ([2'-5']U10) served as a template for the synthesis of oligoadenylates [oligo(A)s] from the 5'-phosphorimidazolide of 2'-5' diadenylate (ImpA-2'p5'A). Joining of [2'-5']U10and ImpA2'p5'A also took place in substantial amounts to yield long-chain oligoribonucleotides in the template-directed reaction. An unusual CD spectrum ascribed to helix formation between [2'-5']U10and [2'-5'](pA)2was observed under the same conditions as that of the template-directed reaction. The 3'-5' linked decauridylate ([3'-5']U10) also promoted the template-directed synthesis of oligo(A)s from ImpA2'p5'A, but more slowly compared with [2'-5']U10. The results indicate that short-chain RNA oligomers with a 2'-5' phosphodiester bond could lead to longer oligoribonucleotides by template-directed chain elongation.
Sawai
H
H
Department of Chemistry, Faculty of Engineering, Gunma University, Kiryu, Gunma 376-8515, Japan. sawai@gunma-u.ac.jp
Totsuka
S
S
Yamamoto
K
K
Ozaki
H
H
D000227:Adenine Nucleotides / Q000737:chemistry; D002942:Circular Dichroism; D009690:Nucleic Acid Conformation; D009843:Oligoribonucleotides / Q000138:chemical synthesis* / Q000737:chemistry; D011061:Poly A / Q000138:chemical synthesis; D011072:Poly U / Q000138:chemical synthesis; D012313:RNA / Q000138:chemical synthesis* / Q000737:chemistry; D013698:Templates, Genetic
D000227:Adenine Nucleotides; D009843:Oligoribonucleotides; D011061:Poly A; D011072:Poly U; C023505:2',5'-oligoadenylate; D012313:RNA