Biochemical and phylogenetic analyses of methionyl-tRNA synthetase isolated from a pathogenic microorganism, Mycobacterium tuberculosis.
Mycobacterium tuberculosis methionyl-tRNA synthetase (MetRS) has been cloned and characterized. The protein contains class I signature sequences but lacks the Zn2+ binding motif and the C-terminal dimerization appendix that are found in MetRSs from several organisms including E. coli MetRS. Consistent with these features, the enzyme behaved as a monomer in a gel filtration chromatography and did not contain the bound Zn2+. Nonetheless, it was active to the tRNAMet of E. coli as determined by in vivo genetic complementation and in vitro reaction. Phylogenetic analysis separated the M. tuberculosis and E. coli MetRSs into prokaryote and eukaryote-archaea group, respectively. This result is consistent with the taxonomic locations of the organism but is an interesting contrast to the case of its paralogous protein, isoleucyl-tRNA synthetase, and suggests that the two enzymes evolved in separate idiosyncratic pathways.
Kim
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Sung Kyun Kwan University, Department of Biology, Suwon, Kyunggido, South Korea. shkim@yurim.skku.ac.kr
Jo
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YJ
Lee
S H
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Motegi
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Shiba
K
K
Sassanfar
M
M
Martinis
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D000595:Amino Acid Sequence; D001665:Binding Sites; D003001:Cloning, Molecular; D019281:Dimerization; D004926:Escherichia coli / Q000201:enzymology / Q000235:genetics; D005816:Genetic Complementation Test; D007700:Kinetics; D008718:Methionine-tRNA Ligase / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D008969:Molecular Sequence Data; D009169:Mycobacterium tuberculosis / Q000201:enzymology* / Q000235:genetics; D010802:Phylogeny; D011487:Protein Conformation; D012358:RNA, Transfer, Met / Q000378:metabolism; D017422:Sequence Analysis, DNA; D015032:Zinc / Q000378:metabolism
D012358:RNA, Transfer, Met; C024988:tRNA, formylmethionine-; D008718:Methionine-tRNA Ligase; D015032:Zinc