Two sites in the Delta gene region contribute to naturally occurring variation in bristle number in Drosophila melanogaster.
A restriction enzyme survey of a 57-kb region including the gene Delta uncovered 53 polymorphic molecular markers in a sample of 55 naturally occurring chromosomes. A permutation test, which assesses the significance of the molecular marker with the largest effect on bristle variation in four genetic backgrounds relative to permuted data-sets, found two sites that were independently associated with variation in bristle number. A common site in the second intron of Delta affected only sternopleural bristle number, and another common site in the fifth intron affected only abdominal bristle number in females. Under an additive genetic model, the polymorphism in the second intron may account for 12% of the total genetic variation in sternopleural bristle number due to third chromosomes, and the site in the fifth intron may account for 6% of the total variation in female abdominal bristle number due to the third chromosomes. These results suggest the following: (1) models that incorporate balancing selection are more consistent with observations than deleterious mutation-selection equilibrium models, (2) mapped quantitative trait loci of large effect may not represent a single variable site at a genetic locus, and (3) linkage disequilibrium can be used as a tool for understanding the molecular basis of quantitative variation.
Long
A D
AD
Center for Population Biology, University of California, Davis, California 95616, USA. tdlong@ucdavis.edu
Lyman
R F
RF
Langley
C H
CH
Mackay
T F
TF
D000818:Animals; D002875:Chromosomes / Q000235:genetics; D017124:Conserved Sequence; D004331:Drosophila melanogaster / Q000235:genetics*; D005260:Female; D017344:Genes, Insect; D005819:Genetic Markers; D014644:Genetic Variation; D047908:Intracellular Signaling Peptides and Proteins; D016013:Likelihood Functions; D016014:Linear Models; D015810:Linkage Disequilibrium; D008297:Male; D008565:Membrane Proteins / Q000235:genetics*; D010641:Phenotype; D012150:Polymorphism, Restriction Fragment Length; D015183:Restriction Mapping
D005819:Genetic Markers; D047908:Intracellular Signaling Peptides and Proteins; D008565:Membrane Proteins; C064045:delta protein