title: Basal cell adhesion molecule/lutheran protein. The receptor critical for sickle cell adhesion to laminin.
abstract: Sickle red cells bind significant amounts of soluble laminin, whereas normal red cells do not. Solid phase assays demonstrate that B-CAM/LU binds laminin on intact sickle red cells and that red cell B-CAM/LU binds immobilized laminin, whereas another putative laminin binding protein, CD44, does not. Ligand blots also identify B-CAM/LU as the only erythrocyte membrane protein(s) that binds laminin. Finally, transfection of murine erythroleukemia cells with human B-CAM cDNA induces binding of both soluble and immobilized laminin. Thus, B-CAM/LU appears to be the major laminin-binding protein of sickle red cells. Previously reported overexpression of B-CAM/LU by epithelial cancer cells suggests that this protein may also serve as a laminin receptor in malignant tumors.
authors.affiliation: Division of Hematology, Department of Medicine, Duke University Medical Center, Duke University, Durham, North Carolina 27710, USA.
authors.forename: M
authors.initials: M
authors.lastname: Udani
authors.forename: Q
authors.initials: Q
authors.lastname: Zen
authors.forename: M
authors.initials: M
authors.lastname: Cottman
authors.forename: N
authors.initials: N
authors.lastname: Leonard
authors.forename: S
authors.initials: S
authors.lastname: Jefferson
authors.forename: C
authors.initials: C
authors.lastname: Daymont
authors.forename: G
authors.initials: G
authors.lastname: Truskey
authors.forename: M J
authors.initials: MJ
authors.lastname: Telen
mesh_terms: D000755:Anemia, Sickle Cell / Q000097:blood*; D000818:Animals; D015153:Blotting, Western; D015815:Cell Adhesion Molecules / Q000378:metabolism*; D004912:Erythrocytes / Q000378:metabolism*; D006801:Humans; D007797:Laminin / Q000378:metabolism*; D008188:Lutheran Blood-Group System; D051379:Mice; D009363:Neoplasm Proteins / Q000378:metabolism*; D017474:Receptors, Laminin / Q000378:metabolism*; D011994:Recombinant Proteins / Q000378:metabolism
chemical_list: C090228:BCAM protein, human; D015815:Cell Adhesion Molecules; D007797:Laminin; D008188:Lutheran Blood-Group System; D009363:Neoplasm Proteins; D017474:Receptors, Laminin; D011994:Recombinant Proteins