Determination of malondialdehyde in rat brain by capillary zone electrophoresis.
A method for determination of malondialdehyde with capillary electrophoresis using UV detection at 267 nm has been developed. The buffer system consisted of 10 mM borax and 0.5 mM CTAB at pH 9.3. Malondialdehyde migrated as the first peak in the electropherogram at 2.6 min. Limit of detection was 1.2 microM corresponding to 7.8 pg. Malondialdehyde was determined before and after stimulating lipid peroxidation with the addition of ferrous ammonium sulphate to homogenates of rat brain tissue. Proteins were precipitated by boiling and removed from the brain homogenates with centrifugation. No further pretreatment was made before injecting the homogenates on the CE system. Non-precipitated homogenates could also be analyzed, but this required washing of the capillary with 0.1 M NaOH before introduction of the next sample.
Olsson
J
J
Department of Analytical Chemistry, Stockholm University, Sweden.
Karlberg
B
B
Kristensson
K
K
Aberg
F
F
D000818:Animals; D001923:Brain Chemistry; D002021:Buffers; D002138:Calibration; D019075:Electrophoresis, Capillary; D005296:Ferrous Compounds / Q000494:pharmacology; D006863:Hydrogen-Ion Concentration; D066298:In Vitro Techniques; D015227:Lipid Peroxidation / Q000187:drug effects; D008297:Male; D008315:Malondialdehyde / Q000032:analysis*; D009419:Nerve Tissue Proteins / Q000737:chemistry / Q000302:isolation & purification; D000644:Quaternary Ammonium Compounds / Q000494:pharmacology; D051381:Rats; D017207:Rats, Sprague-Dawley; D013056:Spectrophotometry, Ultraviolet; D013268:Stimulation, Chemical
D002021:Buffers; D005296:Ferrous Compounds; D009419:Nerve Tissue Proteins; D000644:Quaternary Ammonium Compounds; D008315:Malondialdehyde; C038178:ammonium ferrous sulfate