Crystal structure of a tyrosine phosphorylated STAT-1 dimer bound to DNA.
The crystal structure of the DNA complex of a STAT-1 homodimer has been determined at 2.9 A resolution. STAT-1 utilizes a DNA-binding domain with an immunoglobulin fold, similar to that of NFkappaB and the p53 tumor suppressor protein. The STAT-1 dimer forms a contiguous C-shaped clamp around DNA that is stabilized by reciprocal and highly specific interactions between the SH2 domain of one monomer and the C-terminal segment, phosphorylated on tyrosine, of the other. The phosphotyrosine-binding site of the SH2 domain in each monomer is coupled structurally to the DNA-binding domain, suggesting a potential role for the SH2-phosphotyrosine interaction in the stabilization of DNA interacting elements.
Chen
X
X
The Rockefeller University, New York, New York 10021, USA.
Vinkemeier
U
U
Zhao
Y
Y
Jeruzalmi
D
D
Darnell
J E
JE
Kuriyan
J
J
D000595:Amino Acid Sequence; D003461:Crystallography; D004247:DNA / Q000737:chemistry*; D004268:DNA-Binding Proteins / Q000737:chemistry* / Q000235:genetics; D019281:Dimerization; D006801:Humans; D008958:Models, Molecular; D008968:Molecular Conformation; D008969:Molecular Sequence Data; D016328:NF-kappa B / Q000737:chemistry; D009838:Oligodeoxyribonucleotides / Q000737:chemistry*; D010446:Peptide Fragments / Q000737:chemistry / Q000235:genetics; D010766:Phosphorylation; D019000:Phosphotyrosine / Q000737:chemistry; D011485:Protein Binding; D017434:Protein Structure, Tertiary; D011994:Recombinant Proteins / Q000737:chemistry; D050794:STAT1 Transcription Factor; D017386:Sequence Homology, Amino Acid; D017356:Synchrotrons; D015534:Trans-Activators / Q000737:chemistry* / Q000235:genetics; D016159:Tumor Suppressor Protein p53 / Q000737:chemistry; D018909:src Homology Domains
D004268:DNA-Binding Proteins; D016328:NF-kappa B; D009838:Oligodeoxyribonucleotides; D010446:Peptide Fragments; D011994:Recombinant Proteins; D050794:STAT1 Transcription Factor; C494079:STAT1 protein, human; D015534:Trans-Activators; D016159:Tumor Suppressor Protein p53; D019000:Phosphotyrosine; D004247:DNA