Construction of phylogenetic tree based on G + C contents in DNA and 16S rRNA sequences: example for group 1 of genus Bacillus.
The applicability of the G + C content in DNA in the construction of phylogenetic tree was studied. The group 1 of the genus Bacillus was selected as an object for study. Statistically reliable correlation between evolutionary distances of 16S rRNA sequences (Ei) and parameter Pi named as "GC evolutionary distance" was shown. The value of Pi is the difference between the G + C content in DNA of two species branching from one phylogenetic line. The coefficient of correlation between Ei and Pi equals 0.97 for the representatives of group 1, including Bacillus anthracis, B. cereus, B. circulans, B. firmus, B. lautus, and B. subtilis. Phylogenetic studies of the G + C content in DNA in the genus Bacillus, group 1 representatives has shown that this character can be used in molecular systematics and phylogeny as additional data altogether with the data on 16S rRNA sequences.
Ivanov
V N
VN
Department of Microbiology and General Immunology, Taras Shevchenko Kyiv University, Ukraine.
D001407:Bacillus / Q000145:classification / Q000235:genetics*; D001408:Bacillus anthracis / Q000145:classification / Q000235:genetics; D001409:Bacillus cereus / Q000145:classification / Q000235:genetics; D001412:Bacillus subtilis / Q000145:classification / Q000235:genetics; D003596:Cytosine; D004269:DNA, Bacterial / Q000235:genetics*; D019143:Evolution, Molecular; D006147:Guanine; D010802:Phylogeny; D012329:RNA, Bacterial / Q000235:genetics*; D012336:RNA, Ribosomal, 16S / Q000235:genetics*
D004269:DNA, Bacterial; D012329:RNA, Bacterial; D012336:RNA, Ribosomal, 16S; D006147:Guanine; D003596:Cytosine