Liquid chromatographic method for analysis of All-rac-alpha-tocopheryl acetate and retinyl palmitate in soy-based infant formula using a zero-control reference material (ZRM) as a method development tool.
A liquid chromatographic method is described for analysis of all-rac-alpha-tocopheryl acetate, tocopherols, and retinyl palmitate in soy-based infant formula. The vitamins are extracted in isopropyl alcohol and hexane--ethyl acetate without saponification and quantitated by normal-phase chromatography with fluorescence detection. All-rac-alpha-tocopheryl acetate and retinyl palmitate are quantitated isocratically with mobile phases of 0.5% (v/v) and 0.125% (v/v) isopropyl alcohol in hexane, respectively. Recoveries from zero control reference material soy-based formula averaged 97.2% (n = 25) for retinyl palmitate and 100% (n = 25) for all-rac-alpha-tocopheryl acetate. Coefficients of variation ranged from 1.21 to 2.86% for retinyl palmitate and from 1.49 to 5.16% for all-rac-alpha-tocopheryl acetate. The method provides a rapid, specific, and easily controlled assay for analysis of vitamin A and vitamin E in fortified infant formula. Additionally, the method eliminates use of chlorinated solvents.
Chase
G W
GW
U.S. Food and Drug Administration, Atlanta Center for Nutrient Analysis, GA 30309, USA.
Long
A R
AR
Eitenmiller
R R
RR
D016588:Anticarcinogenic Agents / Q000032:analysis*; D000975:Antioxidants / Q000032:analysis*; D002853:Chromatography, Liquid / Q000379:methods*; D004224:Diterpenes; D005504:Food Analysis / Q000379:methods; D007225:Infant Food / Q000032:analysis*; D012015:Reference Standards; D000084562:Retinyl Esters; D013025:Glycine max; D013050:Spectrometry, Fluorescence; D013237:Stereoisomerism; D024505:Tocopherols; D014801:Vitamin A / Q000031:analogs & derivatives* / Q000032:analysis; D014810:Vitamin E / Q000031:analogs & derivatives* / Q000032:analysis; D024502:alpha-Tocopherol / Q000031:analogs & derivatives*
D016588:Anticarcinogenic Agents; D000975:Antioxidants; D004224:Diterpenes; D000084562:Retinyl Esters; D014801:Vitamin A; D014810:Vitamin E; C014794:retinol palmitate; D024502:alpha-Tocopherol; D024505:Tocopherols