title: Capillary isotachophoresis with fiber-optic Raman spectroscopic detection. Performance and application to ribonucleotides.
abstract: A fiber-optic Raman probe fitted with a microscope objective was used to obtain on-line normal Raman spectra of adenosine 5'-monophosphate, cytidine 5'-monophosphate, guanosine 5'-monophosphate and uridine 5'-monophosphate separated by capillary isotachophoresis. With multimode optical fiber, the system interrograted a 40-micron length of capillary. Fiber-optic coupling facilitated use of an unmodified spectrograph and conventional capillary mounting systems. Raman spectra were excited with a 2W 532 nm NdYVO4, laser as the excitation source, with collection of 1 spectrum per second. Even at 2.10(-5) M initial concentration, Raman spectra were obtained at a good signal-to-noise ratio.
authors.affiliation: University of Michigan, Department of Chemistry, Ann Arbor 48109-1055, USA.
authors.forename: P A
authors.initials: PA
authors.lastname: Walker
authors.forename: M D
authors.initials: MD
authors.lastname: Morris
mesh_terms: D004586:Electrophoresis; D005336:Fiber Optic Technology; D007202:Indicators and Reagents; D007293:Inosine Triphosphate / Q000032:analysis; D055100:Optical Fibers; D012265:Ribonucleotides / Q000032:analysis*; D013059:Spectrum Analysis, Raman
chemical_list: D007202:Indicators and Reagents; D012265:Ribonucleotides; D007293:Inosine Triphosphate