An illustrated guide to RFLP troubleshooting.
A large number of reagents and steps are required for restriction fragment length polymorphism (RFLP) analysis, which at times make determining the cause of any observed anomaly difficult. Troubleshooting problems in RFLP analysis is difficult and often the exact cause of a problem cannot be determined. In this paper a collection of controlled experiments detail the consequences of a number of human or materials problems. Although the focus is on forensic applications, this troubleshooting guide will be helpful to anyone employing Southern analysis.
Benzinger
E A
EA
Ohio Bureau of Criminal Identification and Investigation, London, USA.
Emerick
E
E
Grigsby
N
N
Lovekamp
M L
ML
Logeman
R
R
Sallee
P J
PJ
Boster
K L
KL
Rehnstrom
A
A
Riech
A K
AK
Small
A T
AT
Glasner
J A
JA
Coons
T M
TM
von Beroldingen
C
C
D016477:Artifacts; D001345:Autoradiography; D015139:Blotting, Southern; D002874:Chromosome Mapping / Q000379:methods*; D004247:DNA / Q000302:isolation & purification; D016172:DNA Fingerprinting / Q000379:methods*; D015252:Deoxyribonucleases, Type II Site-Specific / Q000378:metabolism; D004587:Electrophoresis, Agar Gel / Q000379:methods; D006801:Humans; D009691:Nucleic Acid Denaturation; D012150:Polymorphism, Restriction Fragment Length; D012685:Sepharose / Q000737:chemistry
D004247:DNA; D012685:Sepharose; D015252:Deoxyribonucleases, Type II Site-Specific; C024403:GGCC-specific type II deoxyribonucleases