The immune modulator Linomide prevents neuronal death in injured peripheral nerves of the mouse.
Neuronal death after injury or disease could result from imbalanced cytokine expression. Linomide (LS-2616, quinoline-3-carboxamide), a synthetic immunomodulator with effects on cytokine production, suppresses autoimmune diseases of the nervous system. Here adult mice were pre-treated with 200 mg/kg/day of Linomide for 9 days, after which the sciatic nerves were crushed. After another 10 days of Linomide treatment the dorsal root ganglia were dissected out and stained for apoptosis, either immediately or after 2 days in culture, which increases cell death. Superior cervical ganglia were also cultured for 2 days. The Linomide pretreatment profoundly reduced (approximately 60-80%) the injury-induced apoptotic death of neurons and satellite cells in both systems. The results suggest that modulation of the inflammatory cytokine cascade is a promising road to nerve cell rescue.
Ekström
P A
PA
Department of Animal Physiology, University of Lund, Sweden.
Hedlund
G
G
Karlsson
J
J
Andersson
G
G
D000276:Adjuvants, Immunologic / Q000494:pharmacology; D000818:Animals; D017209:Apoptosis / Q000187:drug effects*; D016923:Cell Death / Q000187:drug effects; D005727:Ganglia, Spinal / Q000166:cytology / Q000187:drug effects* / Q000502:physiology; D006912:Hydroxyquinolines / Q000494:pharmacology*; D008297:Male; D051379:Mice; D008815:Mice, Inbred Strains; D009409:Nerve Crush; D009474:Neurons / Q000166:cytology / Q000187:drug effects*; D012584:Sciatic Nerve / Q000166:cytology / Q000187:drug effects* / Q000293:injuries*
D000276:Adjuvants, Immunologic; D006912:Hydroxyquinolines; C045282:roquinimex