Biologically-generated primer for PCR: PCR primer of unknown sequence.
We describe a method for producing specific PCR primers directly from PCR product, bypassing the usual need to know the primer sequence. Lack of abundance of primers derived from a PCR product is compensated for by the incorporation of an arbitrary 5'TAG sequence which acts as a surrogate template target for the bulk amplification phase. We use the technique to amplify clonospecific rearranged immunoglobulin genes, which have applications as markers of lymphoid neoplasms for tracing the success of therapy. The principle may have wider application wherever conserved and variable regions of DNA are juxtaposed.
Bindon
C
C
Department of Paediatric Oncology, Oxford Radcliffe Hospital, Oxford, UK. c.bindon@bad.dundee.ac.uk
Martindale
J
J
Mitchell
C
C
D001483:Base Sequence; D004247:DNA / Q000032:analysis; D017931:DNA Primers; D006801:Humans; D016133:Polymerase Chain Reaction / Q000379:methods*; D012680:Sensitivity and Specificity
D017931:DNA Primers; D004247:DNA