title: Antisense mapping of opioid receptor clones: effects upon 2-deoxy-D-glucose-induced hyperphagia.
abstract: Antisense oligodeoxynucleotides (AS ODNs) directed against exons 1 and 2 of the MOR-1 clone significantly and markedly reduced (81-93%) hyperphagia induced by the anti-metabolic glucose analogue, 2-deoxy-d-glucose (2DG) across a 4 h time course. AS ODNs directed against exons 3 or 4 of the MOR-1 clone had a more limited (1-2 h) duration of action upon 2DG-induced hyperphagia. 2DG-induced hyperphagia was significantly reduced by AS ODNs directed against exon 2 (44-51%), but not exons 1 or 3 of the KOR-1 clone across a 4 h time course. Whereas an AS ODN probe directed against the KOR3/ORL-1 clone produced small (36%), but significant reductions in 2DG-induced hyperphagia, an AS ODN probe directed against the DOR-1 clone was ineffective. These data provide further converging evidence for the roles of primarily mu, but also kappa1 and kappa3 opioid receptors in mediating the hyperphagic effects of glucoprivation.
authors.affiliation: Neuropsychology Doctoral Sub-Program, Queens College, CUNY, Flushing, NY 11367, USA.
authors.forename: K
authors.initials: K
authors.lastname: Burdick
authors.forename: W Z
authors.initials: WZ
authors.lastname: Yu
authors.forename: A
authors.initials: A
authors.lastname: Ragnauth
authors.forename: M
authors.initials: M
authors.lastname: Moroz
authors.forename: Y X
authors.initials: YX
authors.lastname: Pan
authors.forename: G C
authors.initials: GC
authors.lastname: Rossi
authors.forename: G W
authors.initials: GW
authors.lastname: Pasternak
authors.forename: R J
authors.initials: RJ
authors.lastname: Bodnar
mesh_terms: D000818:Animals; D003001:Cloning, Molecular; D003847:Deoxyglucose; D005091:Exons; D006963:Hyperphagia / Q000139:chemically induced*; D008297:Male; D016376:Oligonucleotides, Antisense; D051381:Rats; D017207:Rats, Sprague-Dawley; D011957:Receptors, Opioid / Q000032:analysis*
chemical_list: D016376:Oligonucleotides, Antisense; D011957:Receptors, Opioid; D003847:Deoxyglucose