title: Detection of human papillomavirus type 33 DNA in extragenital Bowen's disease with the polymerase chain reaction.
abstract: BACKGROUND
  Data on the association of human papillomavirus (HPV) infection with extragenital Bowen's disease are very scarce.
  
  
  OBJECTIVE
  To evaluate the prevalence of HPV infection in extragenital Bowen's disease showing histologically a number of koilocytes in the lesional epidermis, we studied formalin-fixed, paraffin-embedded tissues of 9 such cases.
  
  
  METHODS
  HPV DNA was studied in such samples by the polymerase chain reaction (PCR) and in situ hybridization.
  
  
  RESULTS
  Despite negative results with in situ hybridization, the PCR with HPV type 33 primer detected the presence of virus DNA in 2 out of 9 cases.
  
  
  CONCLUSIONS
  As far as we know, this is the first report of the detection of HPV type 33 DNA in the lesional skin of extragenital Bowen's disease. The prevalence of HPV infection in extragenital Bowen's disease may be higher than expected, especially in cases histologically showing many koilocytes in the lesional epidermis.
authors.affiliation: Department of Dermatology, Tohoku University School of Medicine, Sendai, Japan.
authors.forename: M
authors.initials: M
authors.lastname: Deguchi
authors.forename: Y
authors.initials: Y
authors.lastname: Tomioka
authors.forename: M
authors.initials: M
authors.lastname: Mizugaki
authors.forename: H
authors.initials: H
authors.lastname: Tagami
mesh_terms: D000368:Aged; D001913:Bowen's Disease / Q000473:pathology / Q000821:virology*; D017931:DNA Primers; D004279:DNA, Viral / Q000302:isolation & purification; D006801:Humans; D017403:In Situ Hybridization; D008297:Male; D027383:Papillomaviridae / Q000235:genetics / Q000302:isolation & purification*; D030361:Papillomavirus Infections / Q000150:complications*; D016133:Polymerase Chain Reaction; D012878:Skin Neoplasms / Q000473:pathology / Q000821:virology*; D014412:Tumor Virus Infections / Q000150:complications*
chemical_list: D017931:DNA Primers; D004279:DNA, Viral