title: Platelet epidermal growth factor in thyroid disorders.
abstract: We evaluated the concentration of epidermal growth factor (EGF) in platelets, serum and plasma obtained from 47 patients with Graves' disease, 7 with hypothyroidism and 20 healthy subjects. The platelets of the subjects were collected from platelet rich plasma and lysed by freezing and thawing. Subsequently the platelet debris was treated with Triton X-100. The EGF concentration was determined by homologous radioimmunoassay. The concentration of EGF in the platelets in 14 patients with untreated Graves' disease was significantly higher than that in the healthy controls. After treating these 14 patients with antithyroid agents, the EGF concentration in the platelets decreased to the level of the healthy controls. The EGF concentration in the platelets in the 7 untreated hypothyroid patients decreased after replacement therapy with thyroxine. The mean volume of the platelets in the 14 patients with untreated Graves' disease was significantly larger than in the control and decreased after treatment with antithyroid agents. The serum and plasma levels of EGF in the 7 untreated hypothyroid increased after replacement therapy. In conclusion, thyroid function affected the concentration of EGF in the platelets of patients with thyroid disorders.
authors.affiliation: Third Department of Internal Medicine, Nagoya City University Medical School, Nagoya, Japan.
authors.forename: M
authors.initials: M
authors.lastname: Okada
authors.forename: Y
authors.initials: Y
authors.lastname: Kamiya
authors.forename: J
authors.initials: J
authors.lastname: Ito
authors.forename: T
authors.initials: T
authors.lastname: Yoshimata
authors.forename: M
authors.initials: M
authors.lastname: Kawaguchi
authors.forename: H
authors.initials: H
authors.lastname: Shibata
authors.forename: T
authors.initials: T
authors.lastname: Fujinami
mesh_terms: D000328:Adult; D001792:Blood Platelets / Q000378:metabolism*; D004815:Epidermal Growth Factor / Q000097:blood*; D005260:Female; D006111:Graves Disease / Q000097:blood / Q000188:drug therapy; D006801:Humans; D007037:Hypothyroidism / Q000097:blood; D008297:Male; D008713:Methimazole / Q000627:therapeutic use; D008875:Middle Aged; D010976:Platelet Count; D011441:Propylthiouracil / Q000627:therapeutic use; D013959:Thyroid Diseases / Q000097:blood*; D013974:Thyroxine / Q000097:blood
chemical_list: D008713:Methimazole; D004815:Epidermal Growth Factor; D011441:Propylthiouracil; D013974:Thyroxine