Discrimination between different types of human adeno-associated viruses in clinical samples by PCR.
Persistent infection of human tissues with the helper virus-dependent parvovirus, adeno-associated virus (AAV) was detected by polymerase chain reaction (PCR) using primer pairs detecting AAV types 2, 3 or 5. In order to develop PCR protocols which discriminate between the different serotypes of AAV, the DNA of AAV-5 was sequenced partially and compared with the published sequences of AAV-2 and -3. Type specific oligonucleotides and specific probes which allow the distinction between human AAV types by PCR are described.
Tobiasch
E
E
Deutsches Krebsforschungszentrum Angewandte Tumorvirologie 0100, Heidelberg, Germany.
Burguete
T
T
Klein-Bauernschmitt
P
P
Heilbronn
R
R
Schlehofer
J R
JR
D001483:Base Sequence; D017931:DNA Primers / Q000235:genetics; D004279:DNA, Viral / Q000235:genetics*; D000229:Dependovirus / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification; D004587:Electrophoresis, Agar Gel; D004591:Electrophoresis, Polyacrylamide Gel; D006367:HeLa Cells; D006801:Humans; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction / Q000379:methods*; D016415:Sequence Alignment; D017421:Sequence Analysis; D017422:Sequence Analysis, DNA / Q000379:methods*; D012703:Serotyping
D017931:DNA Primers; D004279:DNA, Viral