title: Ca2+ channel sensitivity towards the blocker isradipine is affected by alternative splicing of the human alpha1C subunit gene.
abstract: L-type Ca2+ channels are important targets for drugs, such as dihydropyridines (DHPs), in the treatment of cardiovascular diseases. Channel expression is regulated by alternative splicing. It has been suggested that in the cardiovascular system tissue-specific expression of different L-type Ca2+ channel splice variants may underlie the observed differences in sensitivities to channel block by DHPs. We investigated the sensitivity of Ca2+ channel splice variants derived from the human alpha1C gene to the DHP isradipine. Among seven alpha1C channels we observed up to 10-fold differences in IC50 values for isradipine, as well as changes in the voltage dependence of DHP action.
authors.affiliation: Department of Pharmacology, University of Bern, Switzerland. roger.zuehlke@pki.unibe.ch
authors.forename: R D
authors.initials: RD
authors.lastname: Zühlke
authors.forename: A
authors.initials: A
authors.lastname: Bouron
authors.forename: N M
authors.initials: NM
authors.lastname: Soldatov
authors.forename: H
authors.initials: H
authors.lastname: Reuter
mesh_terms: D017398:Alternative Splicing / Q000502:physiology*; D000818:Animals; D002121:Calcium Channel Blockers / Q000494:pharmacology*; D015220:Calcium Channels / Q000187:drug effects* / Q000235:genetics*; D005091:Exons / Q000235:genetics; D006801:Humans; D017275:Isradipine / Q000494:pharmacology*; D008564:Membrane Potentials / Q000187:drug effects; D008969:Molecular Sequence Data; D018408:Patch-Clamp Techniques; D014982:Xenopus laevis
chemical_list: D002121:Calcium Channel Blockers; D015220:Calcium Channels; D017275:Isradipine