Expression of placental protein 14 by the new endometrial cancer cell line MFE-280 in vitro and by endometrial carcinomas in vivo.
MFE-280 endometrial cancer cells express PP14 (placental protein 14) in vitro. PP14 is normally found in the secretory endometrium and in placental tissue. MFE-280 cells, which are tumorigenic in nude mice, were derived from a recurrent, poorly differentiated endometrial carcinoma. The cells were initially grown in suspension culture and later transferred to monolayer cultures. Karyotyping revealed near-diploidy with a complex heterogeneous aberration pattern. MFE-280 cells were positive for the cytokeratins 7, 8, 18 and 19 as well as for vimentin. The expression of PP14 in MFE-280 cells was demonstrated by immunochemistry and reverse transcriptase--polymerase chain reaction. PP14-mRNA was also detected in one out of five endometrial cancer specimen. In tumor tissue the expression of PP14 was not dependent on progestins.
Hackenberg
R
R
Zentrum für Frauenheilkunde und Geburtshilfe, Philipps-Universität, Marburg, Germany.
Loos
S
S
Nia
A H
AH
Kunzmann
R
R
Schulz
K D
KD
D000818:Animals; D002455:Cell Division; D016889:Endometrial Neoplasms / Q000737:chemistry* / Q000235:genetics / Q000473:pathology; D005260:Female; D000071816:Glycodelin; D006023:Glycoproteins / Q000032:analysis* / Q000235:genetics / Q000502:physiology; D006801:Humans; D007621:Karyotyping; D051379:Mice; D008819:Mice, Nude; D011257:Pregnancy Proteins / Q000032:analysis* / Q000235:genetics / Q000502:physiology; D012333:RNA, Messenger / Q000032:analysis; D011980:Receptors, Progesterone / Q000032:analysis; D014407:Tumor Cells, Cultured
D000071816:Glycodelin; D006023:Glycoproteins; C045246:PAEP protein, human; C489047:Paep protein, mouse; D011257:Pregnancy Proteins; D012333:RNA, Messenger; D011980:Receptors, Progesterone