title: Occurrence of beta 2-adrenergic agonist residues in urine of animal meat producers in Portugal.
abstract: Between January 1, 1991, and December 31, 1993, bovine urine was collected for analysis of beta 2-adrenergic agonist residues. A multiresidue method was developed that was capable of detecting clenbuterol, clenpenterol, clenproperol, mabuterol, mapenterol, bromobuterol, tulobuterol, and salbutamol through enzyme-linked immunosorbent assay, liquid chromatography (LC) with photodiode array detection, and gas chromatography with mass detection in electron impact mode with selective-ion monitoring. Among 1031 samples, 24 contained clenbuterol and 1 contained salbutamol. The results confirm that clenbuterol is the beta 2-adrenergic agonist most used as bovine growth promoter in Portugal.
authors.affiliation: Universidade de Coimbra, Faculdade de Farmácia, Laboratório de Bromatologia e Nutrição, Portugal.
authors.forename: F
authors.initials: F
authors.lastname: Ramos
authors.forename: M C
authors.initials: MC
authors.lastname: Castilho
authors.forename: M I
authors.initials: MI
authors.lastname: Silveira
mesh_terms: D000318:Adrenergic beta-Agonists / Q000652:urine*; D000818:Animals; D002417:Cattle / Q000652:urine*; D002853:Chromatography, Liquid / Q000379:methods; D004350:Drug Residues; D004797:Enzyme-Linked Immunosorbent Assay; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods; D008460:Meat / Q000032:analysis*; D018343:Receptors, Adrenergic, beta-2 / Q000187:drug effects; D015203:Reproducibility of Results
chemical_list: D000318:Adrenergic beta-Agonists; D018343:Receptors, Adrenergic, beta-2