Differential regulation of the AP-1 family members by UV irradiation in vitro and in vivo.
We have examined the effect of UVB and solar-simulated irradiation on the expression of the AP-1 family of transcription factors and the cytokine IL-6 both in cell cultures and in human skin in vivo. UVB irradiation potently induced c-jun, junB and c-fos mRNA levels in vitro in HaCaT cells. IL-6 mRNA was induced in response to UVB irradiation 2-3 h later than c-jun, junB and c-fos mRNAs. In human skin in vivo, solar-simulated irradiation induced transiently junB expression. Genistein, a tyrosine kinase inhibitor, augmented the induction of c-jun and junB by UVB irradiation in HaCaT cells. The results of this study provide evidence that in addition to c-jun and c-fos, junB is also an essential component of the human UV-response. This study also suggests that UVB irradiation regulates the AP-1 family by several mechanisms and that the signalling mechanisms of UVB irradiation are considerably different from the ones used by UVC irradiation.
Isoherranen
K
K
Department of Clinical Chemistry, University of Turku, Finland. kirsi.isoherranen@utu.fi
Westermarck
J
J
Kähäri
V M
VM
Jansén
C
C
Punnonen
K
K
D000111:Acetylcysteine / Q000494:pharmacology; D000328:Adult; D002460:Cell Line; D004791:Enzyme Inhibitors / Q000494:pharmacology; D016166:Free Radical Scavengers / Q000494:pharmacology; D005786:Gene Expression Regulation / Q000528:radiation effects*; D006801:Humans; D015850:Interleukin-6 / Q000235:genetics; D008297:Male; D011505:Protein-Tyrosine Kinases / Q000037:antagonists & inhibitors; D016760:Proto-Oncogene Proteins c-fos / Q000235:genetics*; D016755:Proto-Oncogene Proteins c-jun / Q000235:genetics*; D011519:Proto-Oncogenes / Q000235:genetics; D012333:RNA, Messenger / Q000032:analysis; D012867:Skin / Q000378:metabolism*; D018808:Transcription Factor AP-1 / Q000235:genetics*; D014466:Ultraviolet Rays
D004791:Enzyme Inhibitors; D016166:Free Radical Scavengers; D015850:Interleukin-6; D016760:Proto-Oncogene Proteins c-fos; D016755:Proto-Oncogene Proteins c-jun; D012333:RNA, Messenger; D018808:Transcription Factor AP-1; D011505:Protein-Tyrosine Kinases; D000111:Acetylcysteine