title: Atrial natriuretic peptide increases urodilatin in the circulation.
abstract: BACKGROUND
  Urodilatin is a 32-amino-acid (AA) peptide formed in the kidney.
  
  
  METHODS
  High-performance gel permeation chromatography and high-pressure liquid chromatography evaluation of plasma followed by sensitive urodilatin and atrial natriuretic peptide (ANP) assays revealed that urodilatin does circulate distinctly from ANP.
  
  
  RESULTS
  Urodilatin circulates at very low levels (i.e 9-12 pg/ml). Infusion of ANP increased the circulating concentration of urodilatin 135-fold (p < 0.001), suggesting that some of the effects of ANP may be mediated by urodilatin while long-acting natriuretic peptide, vessel dilator, and kaliuretic peptide did not affect urodilatin in healthy humans (n = 30). Only ANP decreased the renal clearance of urodilatin (60-75%, p < 0.01). Urodilatin was metabolized into peptides smaller than 5 AAs as well as excreted intact into urine.
  
  
  CONCLUSIONS
  Urodilatin circulates and is increased by ANP in humans.
authors.affiliation: Department of Medicine, James A. Haley Veterans Hospital, and University of South Florida Health Sciences Center, Tampa 33612, USA.
authors.forename: D L
authors.initials: DL
authors.lastname: Vesely
authors.forename: R M
authors.initials: RM
authors.lastname: Overton
authors.forename: M
authors.initials: M
authors.lastname: Blankenship
authors.forename: M T
authors.initials: MT
authors.lastname: McCormick
authors.forename: D D
authors.initials: DD
authors.lastname: Schocken
mesh_terms: D000328:Adult; D009320:Atrial Natriuretic Factor / Q000097:blood* / Q000737:chemistry / Q000494:pharmacology*; D002850:Chromatography, Gel; D002851:Chromatography, High Pressure Liquid; D005260:Female; D006801:Humans; D008297:Male; D010446:Peptide Fragments / Q000097:blood* / Q000737:chemistry; D011863:Radioimmunoassay
chemical_list: D010446:Peptide Fragments; C056452:Ularitide; D009320:Atrial Natriuretic Factor