Automated extraction of lysergic acid diethylamide (LSD) and N-demethyl-LSD from blood, serum, plasma, and urine samples using the Zymark RapidTrace with LC/MS/MS confirmation.
A forensic procedure for the quantitative confirmation of lysergic acid diethylamide (LSD) and the qualitative confirmation of its metabolite, N-demethyl-LSD, in blood, serum, plasma, and urine samples is presented. The Zymark RapidTrace was used to perform fully automated solid-phase extractions of all specimen types. After extract evaporation, confirmations were performed using liquid chromatography (LC) followed by positive electrospray ionization (ESI+) mass spectrometry/mass spectrometry (MS/MS) without derivatization. Quantitation of LSD was accomplished using LSD-d3 as an internal standard. The limit of quantitation (LOQ) for LSD was 0.05 ng/mL. The limit of detection (LOD) for both LSD and N-demethyl-LSD was 0.025 ng/mL. The recovery of LSD was greater than 95% at levels of 0.1 ng/mL and 2.0 ng/mL. For LSD at 1.0 ng/mL, the within-run and between-run (different day) relative standard deviation (RSD) was 2.2% and 4.4%, respectively.
de Kanel
J
J
National Medical Services, Inc., Willow Grove, PA, USA.
Vickery
W E
WE
Waldner
B
B
Monahan
R M
RM
Diamond
F X
FX
D001826:Body Fluids / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid; D005554:Forensic Medicine / Q000295:instrumentation* / Q000379:methods; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods; D006213:Hallucinogens / Q000032:analysis* / Q000302:isolation & purification; D006801:Humans; D008238:Lysergic Acid Diethylamide / Q000031:analogs & derivatives* / Q000032:analysis* / Q000302:isolation & purification; D013048:Specimen Handling / Q000379:methods; D015813:Substance Abuse Detection / Q000295:instrumentation* / Q000379:methods
D006213:Hallucinogens; C058401:N-demethyllysergic acid diethylamide; D008238:Lysergic Acid Diethylamide