title: Multiplex PCR for enterotoxigenic, attaching and effacing, and Shiga toxin-producing Escherichia coli strains from calves.
abstract: A multiplex PCR was developed to identify enterotoxigenic, attaching and effacing, and Shiga toxin-producing Escherichia coli strains by amplifying genes encoding K99 and F41 fimbriae, heat-stable enterotoxin a, intimin, and Shiga toxins 1 and 2. This multiplex PCR was specific and sensitive. It will be useful for identification of E. coli strains which cause diarrhea in calves.
authors.affiliation: Department of Pathology, College of Veterinary Medicine, Iowa State University, Ames 50011, USA.
authors.forename: S M
authors.initials: SM
authors.lastname: Franck
authors.forename: B T
authors.initials: BT
authors.lastname: Bosworth
authors.forename: H W
authors.initials: HW
authors.lastname: Moon
mesh_terms: D018829:Adhesins, Bacterial; D000818:Animals; D001425:Bacterial Outer Membrane Proteins / Q000235:genetics; D001427:Bacterial Toxins / Q000096:biosynthesis / Q000235:genetics; D002352:Carrier Proteins; D002417:Cattle; D002418:Cattle Diseases / Q000175:diagnosis*; D004768:Enterotoxins / Q000096:biosynthesis / Q000235:genetics; D004926:Escherichia coli / Q000235:genetics / Q000302:isolation & purification / Q000472:pathogenicity*; D004927:Escherichia coli Infections / Q000175:diagnosis / Q000662:veterinary*; D029968:Escherichia coli Proteins; D010861:Fimbriae, Bacterial / Q000235:genetics; D005798:Genes, Bacterial; D016133:Polymerase Chain Reaction / Q000379:methods*; D012680:Sensitivity and Specificity; D022622:Shiga Toxin 1; D014774:Virulence / Q000235:genetics
chemical_list: D018829:Adhesins, Bacterial; D001425:Bacterial Outer Membrane Proteins; D001427:Bacterial Toxins; D002352:Carrier Proteins; D004768:Enterotoxins; D029968:Escherichia coli Proteins; D022622:Shiga Toxin 1; C073819:eaeA protein, E coli