Targeted gene misexpression in chick limb buds using avian replication-competent retroviruses.
The methods and applications for using avian replication-competent retroviruses to target gene misexpression in the developing limb bud of the chicken are described. These viruses provide the means to exploit the strengths of the chick as a model system in experimental embryology in conjunction with a genetic approach for ectopically expressing a gene of interest. The applications and strengths of the system are detailed. All the steps required to produce a virus carrying a transgene of interest and the methodologies behind designing and carrying out misexpression strategies are outlined, and some useful techniques for analyzing infected embryos are described.
Logan
M
M
Department of Genetics, Harvard Medical School, Boston, Massachusetts 02115, USA.
Tabin
C
C
D000818:Animals; D001358:Avian Sarcoma Viruses / Q000235:genetics*; D001483:Base Sequence; D002642:Chick Embryo; D003001:Cloning, Molecular / Q000379:methods; D015967:Gene Expression Regulation, Viral / Q000235:genetics; D018390:Gene Targeting / Q000379:methods*; D018878:Limb Buds / Q000378:metabolism* / Q000821:virology; D008969:Molecular Sequence Data; D012093:Replicon / Q000235:genetics*