Characterisation of a monoclonal antibody recognising the CD3epsilon chain of the bovine T cell receptor complex.
We describe the characterisation of a monoclonal antibody (mAb), designated MM1A, which reacts with an antigen molecule on the surface of bovine alphabeta and gammadeltaTcR+ T cells. The mAb immunoprecipitated a series of polypeptides of 21 kDa, 22 kDa, 32 kDa, 36 kDa and 44 kDa which is consistent with it recognising the TcR/CD3 complex. COS cells, transfected with a cDNA encoding the bovine CD3epsilon chain, reacted with mAb MM1A indicating that the epitope recognised is on the epsilon chain of the complex and confirming that the mAb recognised bovine CD3.
MacHugh
N D
ND
International Livestock Research Institute, Nairobi, Kenya. n.machugh@cgnet.com
Mburu
J K
JK
Hamilton
M J
MJ
Davis
W C
WC
D000818:Animals; D000911:Antibodies, Monoclonal / Q000032:analysis* / Q000096:biosynthesis; D017252:CD3 Complex / Q000235:genetics / Q000276:immunology*; D019556:COS Cells; D002417:Cattle / Q000276:immunology*; D002461:Cell Line, Transformed; D004591:Electrophoresis, Polyacrylamide Gel / Q000662:veterinary; D018984:Epitopes, T-Lymphocyte / Q000032:analysis; D005434:Flow Cytometry / Q000662:veterinary; D007124:Immunoenzyme Techniques / Q000662:veterinary; D008198:Lymph Nodes / Q000737:chemistry; D051379:Mice; D008970:Molecular Weight; D010455:Peptides / Q000032:analysis; D016693:Receptors, Antigen, T-Cell, alpha-beta / Q000276:immunology*; D016692:Receptors, Antigen, T-Cell, gamma-delta / Q000276:immunology*; D014162:Transfection
D000911:Antibodies, Monoclonal; D017252:CD3 Complex; D018984:Epitopes, T-Lymphocyte; D010455:Peptides; D016693:Receptors, Antigen, T-Cell, alpha-beta; D016692:Receptors, Antigen, T-Cell, gamma-delta