title: Heat resistance of Listeria monocytogenes in dairy products as affected by the growth medium.
abstract: Listeria monocytogenes strains 1151 and Scott A were grown in broth at 30 degrees C and transferred to half cream, double cream and butter stored at 5 degrees C to determine the influence of dairy product composition on heat resistance at 52, 56, 60, 64 and 68 degrees C. Strain 1151 showed a higher heat resistance than strain Scott A. The heat resistance of both strains was higher in the dairy products than in broth, particularly at lower temperatures. A significant difference was observed between log 10 of the D-values in the different dairy products. The D-values obtained for both strains resuspended in all the dairy products would result in efficient elimination of the pathogen at 72.7 degrees C for 15 s. The highest D-value was 11.30 s at 68 degrees C and by using a z-value of 6.71 degrees C it can be determined that at 72.7 degrees C the D-value would be 1.5 s. The 15 s process would therefore achieve 10 log reductions. The effect of growth conditions on the heat resistance at 60 degrees C of L. monocytogenes Scott A was also investigated. When the cells were grown in the diary products themselves, and particularly butter, the heat resistance of Scott A was enhanced; for example, the D-values were 7.15 times higher than in broth. Further studies are required to investigate if this protection against heating exists at higher temperatures, in which case the efficiency of pasteurization treatments or other heat treatments would be considerably lowered.
authors.affiliation: Campden & Chorleywood Food Research Association, Chipping Campden, Gloucestershire, UK.
authors.forename: M A
authors.initials: MA
authors.lastname: Casadei
authors.forename: R
authors.initials: R
authors.lastname: Esteves de Matos
authors.forename: S T
authors.initials: ST
authors.lastname: Harrison
authors.forename: J E
authors.initials: JE
authors.lastname: Gaze
mesh_terms: D000818:Animals; D003611:Dairy Products / Q000382:microbiology*; D005516:Food Microbiology; D006358:Hot Temperature; D008089:Listeria monocytogenes