Immunolocalization of stanniocalcin in human kidney.
Stanniocalcin (STC) is a mammalian polypeptide hormone that appears to play a role in mineral metabolism through its regulatory effects on renal phosphate transport. In this report we have characterized tissue-derived STC in humans and found it to be a glycosylated, disulfide-linked dimer, with similar physical and chemical properties to baculovirus-expressed hormone. The hormone was localized to principal and alpha-intercalated cells in the distal half of the nephron. This is the first homologous demonstration of STC proteins and cells in human tissue.
De Niu
P
P
Department of Physiology, Faculty of Dentistry and Medicine, University of Western Ontario, London, Canada.
Olsen
H S
HS
Gentz
R
R
Wagner
G F
GF
D016367:Baculoviridae / Q000235:genetics; D015153:Blotting, Western; D019281:Dimerization; D004220:Disulfides / Q000737:chemistry; D005260:Female; D006023:Glycoproteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D006728:Hormones / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D006801:Humans; D007150:Immunohistochemistry; D007668:Kidney / Q000166:cytology / Q000378:metabolism*; D008297:Male; D009399:Nephrons / Q000166:cytology / Q000378:metabolism; D011487:Protein Conformation; D011994:Recombinant Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism
D004220:Disulfides; D006023:Glycoproteins; D006728:Hormones; D011994:Recombinant Proteins; C051795:teleocalcin