title: Thermodynamics of inositol hexakisphosphate interaction with human oxyhemoglobin.
abstract: The interaction of inositol hexakisphosphate (IHP) with oxygenated human adult hemoglobin (Hb) was investigated at 25 degreesC. The affinity of IHP for oxygenated Hb is strongly pH-dependent, and potentiometric measurements of proton uptake and release upon IHP addition have shown that over the range between pH 8.0 and pH 6.0 in oxygenated Hb there are three groups of residues that change their pKa values after IHP addition, likely because of their interaction with negative charges of the heterotropic effector. On the basis of previous calculations on the electrostatic properties of human Hb (Matthew, J. B., Hanania, G. I. H., and Gurd, F. R. N. (1979) Biochemistry 18, 1919-1928; Lee, A. W.-m., Karplus, M., Poyart, C., and Bursaux, E. (1988) Biochemistry 27, 1285-1301), two of these groups might be Val1beta and His143beta, which are located in the beta1beta2 dyad axis, where they have been also proposed to interact with 2,3-diphosphoglycerate, whereas the third group does not appear easily identifiable. Calorimetric measurements of the heat associated with IHP binding at different pH values over the same range indicate that IHP binding is mostly enthalpy-driven at pH < 7 and mostly entropy-driven at pH > 7.
authors.affiliation: Consiglio Nazionale delle Ricerche Center for Receptor Chemistry and Institute of Chemistry and Clinical Chemistry, Catholic University of Sacred Heart, Largo F. Vito 1, 00168 Rome, Italy.
authors.forename: I
authors.initials: I
authors.lastname: Messana
authors.forename: M
authors.initials: M
authors.lastname: Angeletti
authors.forename: M
authors.initials: M
authors.lastname: Castagnola
authors.forename: G
authors.initials: G
authors.lastname: De Sanctis
authors.forename: E
authors.initials: E
authors.lastname: Di Stasio
authors.forename: B
authors.initials: B
authors.lastname: Giardina
authors.forename: S
authors.initials: S
authors.lastname: Pucciarelli
authors.forename: M
authors.initials: M
authors.lastname: Coletta
mesh_terms: D000328:Adult; D002151:Calorimetry; D019281:Dimerization; D006801:Humans; D006863:Hydrogen-Ion Concentration; D066298:In Vitro Techniques; D010108:Oxyhemoglobins / Q000378:metabolism*; D010833:Phytic Acid / Q000378:metabolism*; D011199:Potentiometry; D011485:Protein Binding; D055672:Static Electricity; D013816:Thermodynamics
chemical_list: D010108:Oxyhemoglobins; D010833:Phytic Acid