title: Rat liver genesine: a biochemical approach.
abstract: Genesine, the naked autoglucosylating protein involved in glycogen biosynthesis, was partially purified from rat liver and some of its biochemical properties were characterized. Its activity was strongly activated by Mn2+ and two-pH optimums were obtained. UDP-14C-Glc was the preferred glucosyl donor substrate, but also UDP-14C-Xyl was. It was additionally found that more than one glucose was transferred to the protein or to that alpha1,4 glucan already linked to the protein from UDP-Glc. Glucose, maltose, xylose and UDP were inhibitors of genesine activity.
authors.affiliation: Instituto de Investigaciones Bioquímicas Luis F. Leloir Fundación Campomar, Buenos Aires, Argentina.
authors.forename: L R
authors.initials: LR
authors.lastname: Lerner
authors.forename: M
authors.initials: M
authors.lastname: Gil
authors.forename: C R
authors.initials: CR
authors.lastname: Krisman
mesh_terms: D000818:Animals; D050260:Carbohydrate Metabolism; D002412:Cations; D006023:Glycoproteins / Q000737:chemistry / Q000302:isolation & purification*; D016695:Glycosyltransferases; D006863:Hydrogen-Ion Concentration; D008099:Liver / Q000737:chemistry*; D011509:Proteoglycans / Q000096:biosynthesis; D051381:Rats; D013696:Temperature; D013997:Time Factors; D014532:Uridine Diphosphate Glucose / Q000378:metabolism
chemical_list: D002412:Cations; D006023:Glycoproteins; D011509:Proteoglycans; C113502:genesine protein, rat; D016695:Glycosyltransferases; D014532:Uridine Diphosphate Glucose