Hypothesis: aldosterone is synthesized by an alternative pathway during severe sodium depletion. 'A new wine in an old bottle'.
1. The last three steps of aldosterone biosynthesis, 11 beta-hydroxylation, 18-hydroxylation and 18-oxidation, have been demonstrated to be catalysed by one enzyme, which is the cytochrome P450(11 beta) (CYP11B) in cow, pig, sheep and bullfrog or cytochrome P450aldo (CYP11B2) in rat, human, mouse and hamster. 2. The related enzyme P450(11 beta) (CYP11B1) from rat, human, mouse and hamster adrenals displays 11 beta-hydroxylation and 18-hydroxylation activities, but not 18-oxidation activity in vitro. No such enzyme has been reported in the cow, pig or sheep to date. 3. Data showing the dissociation of aldosterone secretion from plasma angiotensin II (AngII) levels indicate the presence of other factor(s) that regulate aldosterone biosynthesis in response to changes in body sodium status. Thus, we propose the existence of a 'sodium status factor' that regulates aldosterone biosynthesis in addition to AngII, K+, adrenocorticotropic hormone and atrial natriuretic peptide. 4. We propose that during severe sodium deficiency there is a switch in the aldosterone pathway to a pathway using 18-hydroxy-deoxycorticosterone (18-OH-DOC) rather than corticosterone as an intermediate. This switch may be mediated via the putative 'sodium status factor'. 5. Two models of the hypothesis will be discussed in this paper: (i) a 'one-enzyme' model; and (ii) a 'two-enzyme' model. 6. The one-enzyme model proposes that P450aldo (P450(11 beta) as in the case of the cow, sheep and pig) changes its enzymatic activity during severe sodium deficiency (i.e. switching to the alternative aldosterone biosynthesis pathway). 7. The two-enzyme model proposes that, under normal circumstances, P450aldo synthesizes aldosterone from deoxycorticosterone, while during severe sodium deficiency the P450(11 beta) provides the substrate (i.e. 18-OH-DOC) for the P450aldo.
Boon
W C
WC
Howard Florey Institute of Experimental Physiology and Medicine, University of Melbourne, Parkville, Victoria, Australia.
McDougall
J G
JG
Coghlan
J P
JP
D000450:Aldosterone / Q000096:biosynthesis*; D000804:Angiotensin II / Q000097:blood; D000818:Animals; D009320:Atrial Natriuretic Factor / Q000097:blood; D002384:Catalysis; D019405:Cytochrome P-450 CYP11B2 / Q000378:metabolism*; D003900:Desoxycorticosterone / Q000031:analogs & derivatives / Q000378:metabolism; D006801:Humans; D011188:Potassium / Q000097:blood; D012964:Sodium / Q000172:deficiency*; D013045:Species Specificity; D013252:Steroid 11-beta-Hydroxylase / Q000378:metabolism*
D000804:Angiotensin II; C042509:18-hydroxydeoxycorticosterone; D003900:Desoxycorticosterone; D000450:Aldosterone; D009320:Atrial Natriuretic Factor; D012964:Sodium; D019405:Cytochrome P-450 CYP11B2; D013252:Steroid 11-beta-Hydroxylase; D011188:Potassium