Role of the CCAAT-binding protein CBF/NF-Y in transcription.
The CCAAT motif is one of the common promoter elements present in the proximal promoter of numerous mammalian genes transcribed by RNA polymerase II. CBF (also called NF-Y and CP1) consists of three different subunits and interacts specifically with the CCAAT motif. In each CBF subunit, the segment needed for formation of the CBF-DNA complex is conserved from yeast to human and, interestingly, the conserved segment of two CBF subunits, CBF-A and CBF-C, are homologous to the histone-fold motif of eukaryotic histones and archaebacterial histone-like protein HMf-2. The histone fold motifs of CBF-A and CBF-C interact with each other to form a heterodimer that associates with CBF-B to form a heterotrimeric CBF molecule, which then binds to DNA.
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Dept of Molecular Genetics, University of Texas M. D. Anderson Cancer Center, Houston 77030, USA. sankar_maity@molgen.mdacc.tmc.edu
de Crombrugghe
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D000595:Amino Acid Sequence; D000818:Animals; D050660:Core Binding Factors; D004268:DNA-Binding Proteins / Q000502:physiology*; D006801:Humans; D008969:Molecular Sequence Data; D009363:Neoplasm Proteins; D011401:Promoter Regions, Genetic; D017386:Sequence Homology, Amino Acid; D014157:Transcription Factors / Q000502:physiology*; D014158:Transcription, Genetic
D050660:Core Binding Factors; D004268:DNA-Binding Proteins; D009363:Neoplasm Proteins; D014157:Transcription Factors