Different effects of phorbol ester derivates on human immunodeficiency virus 1 replication in lymphocytic and monocytic human cells.
The mode of action of the phorbol 12,13-dibutyrate (PDBu) and phorbol 12-myristate 13-acetate (PMA) on the human immunodeficiency virus 1 (HIV-1) replication in human lymphocytes and monocytes was studied. PDBu and PMA appear to have similar effects on the regulation of HIV-1 replication in acutely infected cells. Here we show a significantly increased replication of HIV-1 induced by PDBu and PMA in Molt-4 and Jurkat cells, but a reduced replication in THP-1 and U-937 cells. Moreover, quantitatively different activity of the two derivatives in relation to HIV-1 replication was observed. PDBu proved to be a stronger stimulator or suppressor of HIV-1 replication as compared to PMA. Although the precise mechanism of the activation of HIV-1 replication by phorbol ester derivatives is not clear, it can be assumed that the hydrophilycity of PDBu may cause its stronger effect.
Kalvatchev
Z
Z
Laboratorio de Virus Animales, Instituto Venezolano de Investigaciones Científicas, Caracas, Venezuela.
Walder
R
R
Garzaro
D
D
D015497:HIV-1 / Q000187:drug effects* / Q000502:physiology; D006801:Humans; D019169:Jurkat Cells; D008214:Lymphocytes / Q000821:virology*; D009000:Monocytes / Q000821:virology*; D015240:Phorbol 12,13-Dibutyrate / Q000494:pharmacology*; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology*; D014407:Tumor Cells, Cultured; D014779:Virus Replication / Q000187:drug effects
D015240:Phorbol 12,13-Dibutyrate; D013755:Tetradecanoylphorbol Acetate