Inflammatory potential of C-reactive protein complexes compared to immune complexes.
C-reactive protein (CRP) is an acute phase serum protein that binds to phosphocholine (PC) and to components of damaged tissue. CRP resembles antibody in that it binds to ligands and activates the classical complement pathway. To compare the processing of CRP complexes to that of IgG complexes, we have prepared complexes containing the same ligand, PC-conjugated BSA, and IgG antibody to either BSA or CRP. We previously demonstrated similar complement-mediated binding of these complexes to erythrocyte complement receptors. CRP and IgG also bind to receptors on neutrophils (PMN), providing another possible pathway for clearance of ligands. PMN binding of IgG complexes can lead to activation with damaging inflammatory consequences. In the present report we have used CRP and IgG complexes containing PC-BSA to compare binding to PMN and activation of PMN adherence to endothelial cells. The results indicate that CRP complexes do not activate PMN whereas IgG complexes do. Binding assays indicate that there is substantially greater binding of IgG than CRP complexes to PMN.
Romero
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Department of Molecular Genetics and Microbiology, University of New Mexico, Albuquerque 87131, USA.
Morris
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C
Rodriguez
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M
Du Clos
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TW
Mold
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C
D000818:Animals; D000936:Antigen-Antibody Complex / Q000276:immunology* / Q000378:metabolism; D002097:C-Reactive Protein / Q000276:immunology* / Q000378:metabolism; D002448:Cell Adhesion / Q000502:physiology; D003167:Complement Activation / Q000276:immunology; D004730:Endothelium, Vascular / Q000166:cytology / Q000378:metabolism / Q000648:ultrastructure; D006801:Humans; D007074:Immunoglobulin G / Q000276:immunology / Q000378:metabolism; D007249:Inflammation / Q000276:immunology* / Q000378:metabolism; D018375:Neutrophil Activation; D009504:Neutrophils / Q000166:cytology / Q000378:metabolism / Q000648:ultrastructure; D010713:Phosphatidylcholines / Q000276:immunology / Q000378:metabolism; D011817:Rabbits; D011951:Receptors, Complement / Q000378:metabolism; D012709:Serum Albumin / Q000276:immunology / Q000378:metabolism
D000936:Antigen-Antibody Complex; D007074:Immunoglobulin G; D010713:Phosphatidylcholines; D011951:Receptors, Complement; D012709:Serum Albumin; D002097:C-Reactive Protein