Structure-related emission spectrometric analysis of the chemiluminescence of catechins, theaflavins and anthocyanins.
This study for the first time achieved an emission spectrometric analysis of the chemiluminescence of flavonoids in the presence of hydrogen peroxide, acetaldehyde and horseradish peroxidase, and revealed that the maximum emission wavelengths (Emax) strictly differ among catechins (E-max 630 nm), theaflavins (Emax 690 nm) and anthocyanins (Emax 675 nm) according to their chemical structures. This technique enabled the direct incorporation of dietary tea catechin into rat intestinal mucosal cells to be spectrometrically confirmed.
Miyazawa
T
T
Department of Applied Biological Chemistry, Faculty of Agriculture, Tohoku University, Sendai, Japan.
Nakagawa
K
K
D000079:Acetaldehyde; D000818:Animals; D000872:Anthocyanins / Q000737:chemistry* / Q000378:metabolism; D000975:Antioxidants / Q000737:chemistry* / Q000378:metabolism; D044946:Biflavonoids; D002392:Catechin / Q000737:chemistry* / Q000378:metabolism; D006735:Horseradish Peroxidase; D006861:Hydrogen Peroxide; D007202:Indicators and Reagents; D007413:Intestinal Mucosa / Q000737:chemistry / Q000378:metabolism; D008163:Luminescent Measurements; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley; D013055:Spectrophotometry, Infrared; D013662:Tea / Q000737:chemistry
D000872:Anthocyanins; D000975:Antioxidants; D044946:Biflavonoids; D007202:Indicators and Reagents; D013662:Tea; C056068:theaflavin; D002392:Catechin; D006861:Hydrogen Peroxide; D006735:Horseradish Peroxidase; D000079:Acetaldehyde