The recombinant nucleocapsid protein of classical swine fever virus can act as a transcriptional regulator.
The cDNA of the nucleocapsid (core) protein of classical swine fever virus (CSFV) was generated by reverse transcription-polymerase chain reaction (RT-PCR) and cloned into a eukaryotic expression vector. The effect of the recombinant core protein on the transcriptional regulation of cellular as well as viral promoters was studied. Using transient transfection assay, our results demonstrated that the core protein can activate the promoter of human heat shock protein 70 gene, and suppressed the SV40 early promoter. These findings indicate that the core protein appears to function not only as a viral structural protein but also as a regulator of gene expression. The implications of core proteins on the viral maturation are discussed.
Liu
J J
JJ
Department of Veterinary Medicine, National Chung-Hsing University, Taichung, Taiwan, ROC.
Wong
M L
ML
Chang
T J
TJ
D000818:Animals; D002460:Cell Line; D006692:Classical Swine Fever Virus / Q000235:genetics*; D015967:Gene Expression Regulation, Viral; D018840:HSP70 Heat-Shock Proteins / Q000235:genetics / Q000378:metabolism; D006367:HeLa Cells; D006801:Humans; D019590:Nucleocapsid Proteins / Q000737:chemistry / Q000235:genetics / Q000502:physiology*; D010957:Plasmids / Q000235:genetics; D016133:Polymerase Chain Reaction; D011401:Promoter Regions, Genetic; D012367:RNA, Viral / Q000032:analysis; D011994:Recombinant Proteins; D013539:Simian virus 40 / Q000235:genetics; D013552:Swine; D014157:Transcription Factors / Q000737:chemistry / Q000235:genetics / Q000502:physiology*; D014158:Transcription, Genetic; D014162:Transfection
D018840:HSP70 Heat-Shock Proteins; D019590:Nucleocapsid Proteins; D012367:RNA, Viral; D011994:Recombinant Proteins; D014157:Transcription Factors