[Novel site-specific endonucleases from Brevibacterium species].
New site-specific endonucleases BecAI and BecAII have been detected in Brevibacterium species A. Endonuclease BecAII free from contaminating nonspecific endonucleases, exonucleases, and phosphatases was isolated by column chromatography on phosphocellulose, heparin sepharose, and DNA cellulose. It recognizes and cleaves the 5'-GG decreases CC-3' sequence and is a true isoschizomer of HaeIII restriction enzyme. The other restriction endonuclease, BecAI, cleaves Ad2 DNA at least by 2 sites but not the DNA of phage lambda, T7, SV40, phiX174, and plasmides pBR322 and pUC19. The substrate specificity of BecAI indicates its appurtenance to the super rare restriction endonucleases.
Sokolov
N N
NN
Korolev
S V
SV
Rina
M
M
El'darov
M A
MA
Gervaziev
Iu V
IuV
Skriabin
K G
KG
Bouriotis
V
V
D001951:Brevibacterium / Q000201:enzymology*; D015252:Deoxyribonucleases, Type II Site-Specific / Q000235:genetics / Q000302:isolation & purification*; D006868:Hydrolysis; D013379:Substrate Specificity
C113207:endodeoxyribonuclease BecAI; D015252:Deoxyribonucleases, Type II Site-Specific; C024403:GGCC-specific type II deoxyribonucleases
Novye saĭt-spetsificheskie éndonukleazy Brevibacterium species.