title: [An immunochemical study of the antigens from Toxoplasma gondii tachyzoites obtained in different cultivation systems].
abstract: The protein composition and immunochemical properties of Toxoplasma gondii tachyzoites cultured on Vero cells and on mice were studied. Despite the fact that the main components of both preparations were shown to be proteins with molecular weights of 47, 34, 24, and 22 kDa, Toxoplasma-infected human sera antibodies interact mainly with the antigens of 66, 62, 57, 42, 38, 37, 36, 31, and 24 kDa. Comparing efficiency of enzyme immunoassay using the antigens of the tachyzoites obtained in different culture systems showed that the preparation of cultured Vero cells is similar to those of peritoneal exudates from infected mice and may be successfully used for the detection of antitoxoplasma antibodies in the sera of infected subjects.
authors.forename: S E
authors.initials: SE
authors.lastname: Gladkova
authors.forename: N I
authors.initials: NI
authors.lastname: Bormotov
authors.forename: L M
authors.initials: LM
authors.lastname: Dedkova
authors.forename: S S
authors.initials: SS
authors.lastname: Reshetnikov
authors.forename: T B
authors.initials: TB
authors.lastname: Kurlaeva
authors.forename: E F
authors.initials: EF
authors.lastname: Belanov
mesh_terms: D000818:Animals; D000913:Antibodies, Protozoan / Q000097:blood; D000953:Antigens, Protozoan / Q000032:analysis* / Q000302:isolation & purification; D001202:Ascitic Fluid / Q000469:parasitology; D002522:Chlorocebus aethiops; D004591:Electrophoresis, Polyacrylamide Gel; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D007120:Immunochemistry; D051379:Mice; D008970:Molecular Weight; D015800:Protozoan Proteins / Q000032:analysis / Q000302:isolation & purification; D014122:Toxoplasma / Q000254:growth & development / Q000276:immunology*; D014124:Toxoplasmosis, Animal / Q000469:parasitology; D014709:Vero Cells
chemical_list: D000913:Antibodies, Protozoan; D000953:Antigens, Protozoan; D015800:Protozoan Proteins
vernacular_title: Immunokhimicheskoe izuchenie antigenov takhizoitov Toxoplasma gondii, poluchennykh v razlichnykh sistemakh kul'tivirovaniia.