Demonstration of identical expanded clones within both CD8+CD28+ and CD8+CD28- T cell subsets in HIV type 1-infected individuals.
In HIV-1-infected individuals, the CD8+CD28- T cell subset is considerably expanded and is frequently the largest subset of T cells found in peripheral blood. It has been assumed, but not proven, that CD8+CD28- T cells derive from CD8+CD28+ T cells in vivo. To further study the ontogeny of CD8+CD28- T cells, we have performed analyses of the complementarity determining region 3 (CDR3) of the TCRB of CD8+CD28+ and CD8+CD28- T cells from the peripheral blood of HIV-1-infected individuals. When cells from the same individual were compared, expanded peaks in CDR3 length analysis within a given BV family were frequently observed at the same location in both CD8+ subsets (p < 0.001). Sequencing of cDNA corresponding to dominant peaks revealed the presence of identical expanded CD8+ T cell clones within both the CD28+ and CD28- subsets on eight of nine attempts. Our results show that CD8+CD28+ and CD8+CD28- T cells are phenotypic variants of the same lineage, most likely evolving from CD8+CD28+ to end-stage CD8+CD28- T cells.
Mugnaini
E N
EN
Institute of Transplantation Immunology, Rikshospitalet National Hospital, Oslo, Norway.
Spurkland
A
A
Egeland
T
T
Sannes
M
M
Brinchmann
J E
JE
D000328:Adult; D000595:Amino Acid Sequence; D018106:CD28 Antigens / Q000097:blood*; D016827:CD8 Antigens / Q000097:blood*; D002454:Cell Differentiation / Q000276:immunology; D002999:Clone Cells; D015658:HIV Infections / Q000276:immunology* / Q000378:metabolism; D015497:HIV-1 / Q000276:immunology*; D006801:Humans; D008969:Molecular Sequence Data; D017260:Receptor-CD3 Complex, Antigen, T-Cell / Q000032:analysis; D016176:T-Lymphocyte Subsets / Q000737:chemistry / Q000166:cytology* / Q000276:immunology*
D018106:CD28 Antigens; D016827:CD8 Antigens; D017260:Receptor-CD3 Complex, Antigen, T-Cell