Clinical manifestations due to a point mutation of the mitochondrial tRNAleu(UUR) gene in five families with diabetes mellitus.
It has been shown that an adenine (A) to guanine (G) transition at position 3243 of the mitochondrial transfer RNA(tRNA)leu(UUR) gene is associated with a subgroup of diabetes mellitus. Therefore, we screened for this transition in 86 patients with non-insulin-dependent diabetes mellitus (NIDDM) in which two or three generations were affected with diabetes, in 14 patients with insulin-dependent diabetes mellitus, and in 9 families with diabetes mellitus and/or associated disorders suggesting mitochondrial gene abnormalities. We failed to identify the mutation in 100 diabetic patients, 86 NIDDM and 14 insulin-dependent diabetes mellitus (IDDM). Out of the latter 9 families, we identified an A to G transition in 14 individuals in 5 families. Diabetes mellitus was shown to be maternally inherited in one family. In 9 of 14 patients with the mutation, insulin was required to treat diabetes mellitus, indicating impaired insulin secretion. A hyperglycemic clamp test performed in one subject revealed significant impairment of insulin secretion, whereas euglycemic clamp test showed normal insulin sensitivity in this patient. The heteroplasmy of the mutant mitochondrial DNA (mtDNA) in leukocytes does not appear to correlate with the severity of diabetes in terms of the insulin therapy required. Body mass index of the affected individuals was less than 23.3. In one family, in addition to diabetes mellitus and hearing loss, hypoparathyroidism was associated with the mutation, suggesting that hypoparathyroidism is caused by the impaired processing and/or secretion of proparathyroid hormone due to the mutation. In addition, the affected subjects presented with proteinuria at the time of diagnosis of diabetes mellitus which appeared not to be related with diabetic nephropathy.
Shigemoto
M
M
Department of Medicine and Clinical Science, Kyoto University Graduate School of Medicine.
Yoshimasa
Y
Y
Yamamoto
Y
Y
Hayashi
T
T
Suga
J
J
Inoue
G
G
Okamoto
M
M
Jingami
H
H
Tsuda
K
K
Yamamoto
T
T
Yagura
T
T
Oishi
M
M
Tsujii
S
S
Kuzuya
H
H
Nakao
K
K
D000293:Adolescent; D000328:Adult; D000368:Aged; D001786:Blood Glucose / Q000378:metabolism; D017931:DNA Primers / Q000737:chemistry; D004272:DNA, Mitochondrial / Q000235:genetics*; D048909:Diabetes Complications; D003920:Diabetes Mellitus / Q000097:blood / Q000235:genetics*; D005260:Female; D005838:Genotype; D015309:Glucose Clamp Technique; D006801:Humans; D007011:Hypoparathyroidism / Q000097:blood / Q000150:complications / Q000235:genetics; D007328:Insulin / Q000097:blood / Q000378:metabolism; D000078790:Insulin Secretion; D008297:Male; D008875:Middle Aged; D010281:Parathyroid Hormone / Q000097:blood; D010375:Pedigree; D017354:Point Mutation; D016133:Polymerase Chain Reaction; D012356:RNA, Transfer, Leu / Q000235:genetics*
D001786:Blood Glucose; D017931:DNA Primers; D004272:DNA, Mitochondrial; D007328:Insulin; D010281:Parathyroid Hormone; D012356:RNA, Transfer, Leu