Sodium dodecyl sulfate-agarose gel electrophoresis of urinary proteins: application to multiple myeloma.
We evaluated a new sodium dodecyl sulfate-agarose gel electrophoresis (SDS-AGE) for urinary protein analysis in patients with multiple myeloma (MM; n = 47; ages, 62 +/- 2 years, mean +/- SE). Abnormal proteinuria (mean = 1872 +/- 360 mg/24 h) was present in 95% of the samples; 75% of the patients had some sign of renal dysfunction (glomerular and/or tubular) according to their SDS-AGE pattern. A band suggesting Bence Jones proteinuria (BJP) was detected in 40 vs 33 specimens by routine AGE. Immunofixation identified BJP in 38 patients; the calculated sensitivity of SDS-AGE for BJP was 97%. Excellent correlation (P <0.0001) was obtained with routine AGE (r = 0.994) and immunonephelometry (r = 0.963) for light chain quantification. SDS-AGE allows easy evaluation of renal dysfunction and shows high sensitivity for BJP detection. In a specialized laboratory, it is useful for following the progress of MM patients through the semiquantification of BJP.
Le Bricon
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Laboratoire de Biochimie A, Hôpital St-Louis, Paris, France. Biochimie-a@chu-stlouis.fr
Erlich
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Bengoufa
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Dussaucy
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Garnier
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JP
Bousquet
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B
D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D001536:Bence Jones Protein / Q000652:urine*; D004587:Electrophoresis, Agar Gel / Q000379:methods; D005260:Female; D006801:Humans; D007122:Immunoelectrophoresis; D007147:Immunoglobulin Light Chains / Q000652:urine; D008297:Male; D008875:Middle Aged; D009101:Multiple Myeloma / Q000150:complications / Q000652:urine*; D009391:Nephelometry and Turbidimetry / Q000379:methods; D011507:Proteinuria / Q000150:complications / Q000652:urine*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D012967:Sodium Dodecyl Sulfate
D007147:Immunoglobulin Light Chains; D012967:Sodium Dodecyl Sulfate; D001536:Bence Jones Protein