High-performance liquid chromatographic quantitation of total and lactone 20(S)camptothecin in patients receiving oral 20(S)camptothecin.
We have developed a sensitive high-performance liquid chromatography (HPLC) assay to quantitate total and lactone forms of 20(S)camptothecin (CPT) in human plasma. Lactone and total CPT were extracted using solid-phase extraction and liquid-liquid extraction, respectively. The extracted lactone samples could be stored without immediate HPLC analysis. The two forms of CPT were quantitated by reversed-phase HPLC with fluorescence detection. The extraction efficiencies were about 100% and 92% for the total and lactone forms, respectively. The lower limit of quantitation was 5.74 nM for the two forms. The method was reproducible with a mean interday and intraday variability of 6% for total CPT and 4% and 6%. respectively, for lactone CPT. The assay could effectively quantitate lactone and total CPT in patients receiving single dose and multiple doses of oral CPT.
Ahmed
F
F
UMDNJ--The Cancer Institute of New Jersey, New Brunswick 08901, USA.
Vyas
V
V
Saleem
A
A
Li
X G
XG
Zamek
R
R
Cornfield
A
A
Haluska
P
P
Ibrahim
N
N
Rubin
E H
EH
Gupta
E
E
D000972:Antineoplastic Agents, Phytogenic / Q000097:blood* / Q000493:pharmacokinetics; D019540:Area Under Curve; D002166:Camptothecin / Q000097:blood* / Q000493:pharmacokinetics; D002851:Chromatography, High Pressure Liquid; D006207:Half-Life; D006801:Humans; D007783:Lactones / Q000097:blood* / Q000493:pharmacokinetics; D012015:Reference Standards; D015203:Reproducibility of Results; D013048:Specimen Handling; D013050:Spectrometry, Fluorescence
D000972:Antineoplastic Agents, Phytogenic; D007783:Lactones; D002166:Camptothecin