Requirement of the IL-2 receptor beta chain for the development of Vgamma3 dendritic epidermal T cells.
Vgamma3 TCR cells develop in the fetal thymus and migrate to the skin as dendritic epidermal T cells (DETC). Fetal Vgamma3 thymocytes differentiate from immature heat stable antigen (HSA)high cells to mature HSAlow cells and the latter subset predominantly expresses IL-2 receptor beta chain (IL-2Rbeta). In this study, the role of IL-2Rbeta in the development of Vgamma3 cells was determined in IL-2Rbeta-deficient mice. There was a moderate reduction of mature HSAlow Vgamma3 thymocytes in IL-2Rbeta-deficient mice. Small numbers of Vgamma3 DETC were detected in the fetal skin of IL-2Rbeta-deficient mice, but they were absent in newborn and adult mice. These results suggest that IL-2Rbeta may transduce the crucial signal for survival and/or expansion of Vgama3 cells in the fetal thymus and in the fetal skin. In normal mice, IL-15 but not IL-2 mRNA was expressed in the fetal epidermis and exogenous addition of low concentration of IL-15 to fetal skin organ culture induced proliferation of Vgamma3 DETC. The dependence of fetal Vgamma3 DETC on the expression of IL-2Rbeta and the presence of IL-15 mRNA in the fetal epidermis imply an essential role of IL-15 signaling through IL-2Rbeta in the selective localization of this gammadelta T cell subpopulation in the skin.
Kawai
K
K
Department of Dermatology, Niigata University School of Medicine, Japan.
Suzuki
H
H
Tomiyama
K
K
Minagawa
M
M
Mak
T W
TW
Ohashi
P S
PS
D000818:Animals; D000945:Antigens, Differentiation, T-Lymphocyte / Q000276:immunology / Q000378:metabolism; D002455:Cell Division / Q000187:drug effects; D003713:Dendritic Cells / Q000166:cytology / Q000187:drug effects / Q000502:physiology*; D000078404:Epidermal Cells; D004817:Epidermis / Q000737:chemistry / Q000378:metabolism; D005333:Fetus / Q000166:cytology / Q000502:physiology; D007135:Immunoglobulin Variable Region / Q000378:metabolism*; D007376:Interleukin-2 / Q000235:genetics; D015848:Interleukin-5 / Q000235:genetics / Q000494:pharmacology; D018655:Lymphocyte Count; D051379:Mice; D008810:Mice, Inbred C57BL; D008817:Mice, Mutant Strains; D009154:Mutation / Q000235:genetics / Q000502:physiology; D009924:Organ Culture Techniques; D012333:RNA, Messenger / Q000032:analysis / Q000235:genetics; D016692:Receptors, Antigen, T-Cell, gamma-delta / Q000276:immunology / Q000502:physiology*; D015375:Receptors, Interleukin-2 / Q000235:genetics / Q000502:physiology*; D012867:Skin / Q000166:cytology / Q000196:embryology / Q000378:metabolism; D013601:T-Lymphocytes / Q000187:drug effects / Q000276:immunology / Q000502:physiology*; D013950:Thymus Gland / Q000166:cytology / Q000502:physiology
D000945:Antigens, Differentiation, T-Lymphocyte; D007135:Immunoglobulin Variable Region; D007376:Interleukin-2; D015848:Interleukin-5; D012333:RNA, Messenger; D016692:Receptors, Antigen, T-Cell, gamma-delta; D015375:Receptors, Interleukin-2