Determination of the recognition sequence of the type II restriction endonuclease, LlaCI, from Lactococcus lactis W15.
A new type II restriction endonuclease, called LlaCI, was partially purified from Lactococcus lactis subsp. cremoris W15. The characterisation of the LlaCI endonuclease showed it to be an isoschizomer of HindIII, recognising the sequence 5-'A decreases AGCTT-3'. The cleavage site is indicated by the arrow.
Josephsen
J
J
Department of Dairy and Food Science, Royal Veterinary and Agricultural University, Frederiksberg, Denmark. jyj@kvl.dk
Jørgen-Jensen
B
B
Nyengaard
N R
NR
D010582:Bacteriophage lambda; D002847:Chromatography, Agarose; D004279:DNA, Viral; D015247:Deoxyribonuclease HindIII / Q000737:chemistry* / Q000302:isolation & purification / Q000378:metabolism; D015252:Deoxyribonucleases, Type II Site-Specific / Q000737:chemistry* / Q000302:isolation & purification; D013294:Lactococcus lactis / Q000201:enzymology*; D013379:Substrate Specificity
D004279:DNA, Viral; D015247:Deoxyribonuclease HindIII; D015252:Deoxyribonucleases, Type II Site-Specific