Isolation of lipolytic substances caffeine and 1,7-dimethylxanthine from the stem and rhizome of Sinomenium actum.
We attempted to isolate lipolytic substances from the stem and rhizome of Sinomenium actum Rehder et Wilson by using high-performance liquid chromatography (HPLC). S-I and S-II were isolated from the fractions showing lipolytic activity. S-I and S-II were identified as caffeine and 1,7-dimethylxanthine, respectively, by direct comparison with authentic samples. Caffeine (S-I) dose-dependently stimulated lipolytic activity in isolated fat cells of rats, at concentrations of 500 to 1000 microM. 1,7-Dimethylxanthine (S-II) also stimulated lipolytic activity at concentrations of 500 to 1000 microM. Furthermore, we found that caffeine and 1,7-dimethylxanthine enhanced catecholamine-induced lipolysis at lower concentrations of 0.1 to 1 microM.
Jiang
M
M
Kameda
K
K
Han
L K
LK
Kimura
Y
Y
Okuda
H
H
D000818:Animals; D002110:Caffeine / Q000302:isolation & purification*; D002851:Chromatography, High Pressure Liquid; D008066:Lipolysis; D008297:Male; D008516:Medicine, Chinese Traditional; D018547:Plant Stems / Q000737:chemistry*; D010946:Plants, Medicinal / Q000737:chemistry*; D051381:Rats; D017208:Rats, Wistar; D013806:Theophylline / Q000302:isolation & purification*
D002110:Caffeine; D013806:Theophylline; C021183:1,7-dimethylxanthine