title: Progress on the structure and function of aquaporin 1.
abstract: Life exists in water as universal solvent, and cells need to deal with its influx and efflux. Nature has accomplished the almost impossible, creating membrane channels with both a high flux and a high specificity for water. The first water channel was discovered in red blood cell membranes. Today known as aquaporin-1, this channel was found to be closely related to the major integral protein (MIP)1 of the eye lens. Cloning and sequencing of numerous related proteins of the MIP family revealed the widespread occurrence of such channels, suggesting an essential physiological function. Their structures hold the clues to the remarkable water channel activity, as well as to the arrangement of transmembrane segments in general. Recent medium-resolution three-dimensional electron microscopic studies determined a tetrameric complex with six tilted transmembrane helices per monomer. The helices within each monomer surround a central density formed by two interhelical loops implicated by mutagenesis in the water channel function. A combination of sequence analysis and assignment of the observed densities to predicted helices provides a basis for speculation on the nature of the water course through the protein. In particular, four highly conserved polar residues, E142-N192-N76-E17, are proposed to form a chain of key groups involved in the pathway of water flow through the channel.
authors.affiliation: M. E. Müller-Institute for Microscopic Structural Biology, Biozentrum, University of Basel, Switzerland.
authors.forename: J B
authors.initials: JB
authors.lastname: Heymann
authors.forename: P
authors.initials: P
authors.lastname: Agre
authors.forename: A
authors.initials: A
authors.lastname: Engel
mesh_terms: D000818:Animals; D051398:Aquaporin 1; D020346:Aquaporins; D001789:Blood Group Antigens; D002417:Cattle; D003460:Crystallization; D018360:Crystallography, X-Ray; D004910:Erythrocyte Membrane / Q000737:chemistry; D006031:Glycosylation; D006801:Humans; D007473:Ion Channels / Q000737:chemistry* / Q000502:physiology; D008958:Models, Molecular; D005810:Multigene Family; D011487:Protein Conformation; D011499:Protein Processing, Post-Translational; D017433:Protein Structure, Secondary; D011993:Recombinant Fusion Proteins / Q000737:chemistry / Q000378:metabolism; D013329:Structure-Activity Relationship; D014867:Water / Q000378:metabolism*
chemical_list: C496020:AQP1 protein, human; D020346:Aquaporins; D001789:Blood Group Antigens; D007473:Ion Channels; D011993:Recombinant Fusion Proteins; D014867:Water; D051398:Aquaporin 1