title: Steroid hormones modify nuclear heterochromatin structure and plasma membrane enzyme of MCF-7 cells. A combined fractal, electron microscopical and enzymatic analysis.
abstract: Ultrastructural features of the nuclear membrane envelope (ENM) and the nuclear membrane-bound heterochromatin (NMBHC) were investigated in MCF-7 human breast cancer cells by fractal morphometry. The fractal dimension D established by the box counting method proved to be effective for quantifying nuclear changes in MCF-7 cells treated with steroid hormones, namely the estrogen 17 beta-estradiol, which stimulates cell proliferation, and the glucocorticoid dexamethasone. When MCF-7 cells were briefly (5 min) cultured in the presence of 17 beta-estradiol (10(-9) M), the irregularity of the NMBHC outline was increased as documented by the increased fractal dimension D. Changes in the ultrastructural complexity of the nuclear heterochromatin were observed in concomitance with functional changes at the cell periphery, namely the modulation of the estrogen-induced activity of phospholipase C, a cell membrane-associated enzyme involved in the signal transduction pathway via phosphoinositides metabolism. Dexamethasone did not affect the in vitro proliferation, the phospholipase C activity nor the shape of the ENM of MCF-7 cells, but reduced the structural complexity of the nuclear membrane-bound heterochromatin.
authors.affiliation: Laboratorio di Patologia Cellulare, Istituto di Patologia, Locarno, Switzerland.
authors.forename: G A
authors.initials: GA
authors.lastname: Losa
authors.forename: R
authors.initials: R
authors.lastname: Graber
authors.forename: G
authors.initials: G
authors.lastname: Baumann
authors.forename: T F
authors.initials: TF
authors.lastname: Nonnenmacher
mesh_terms: D002462:Cell Membrane / Q000187:drug effects / Q000201:enzymology; D003907:Dexamethasone / Q000494:pharmacology*; D004958:Estradiol / Q000494:pharmacology*; D006570:Heterochromatin / Q000187:drug effects / Q000648:ultrastructure*; D006801:Humans; D054801:Phosphoinositide Phospholipase C; D010727:Phosphoric Diester Hydrolases / Q000378:metabolism*; D014407:Tumor Cells, Cultured
chemical_list: D006570:Heterochromatin; D004958:Estradiol; D003907:Dexamethasone; D010727:Phosphoric Diester Hydrolases; D054801:Phosphoinositide Phospholipase C