title: [Cloning the BR-C gene in Drosophila virilis].
abstract: In 1980, Belyaeva et al. found in Drosophila melanogaster the ecs (ecdysterone sensitivity) locus, later designated BR-C (Broad-Complex). This locus plays a key role in the reaction cascade of ecdysterone-regulated development. The gene BR-C was cloned by Chao and Guild (1986) and Belyaeva et al. (1987). To conduct "walking along the chromosome" in Drosophila virilis, cloned sequences of the BR-C gene from D. melanogaster were used as probes, hybridizing the start sites. A fragment of about 100 kb containing BR-C of D. virilis was cloned. A restriction map of the cloned region was created.
authors.affiliation: Institute of Gene Biology, Russian Academy of Sciences, Moscow, Russia.
authors.forename: S G
authors.initials: SG
authors.lastname: Doronkin
authors.forename: L I
authors.initials: LI
authors.lastname: Korochkin
mesh_terms: D000818:Animals; D015342:DNA Probes; D004330:Drosophila / Q000235:genetics* / Q000254:growth & development; D029721:Drosophila Proteins; D004441:Ecdysterone / Q000502:physiology; D009693:Nucleic Acid Hybridization; D015183:Restriction Mapping; D014157:Transcription Factors / Q000235:genetics*; D016335:Zinc Fingers
chemical_list: C102117:Br protein, Drosophila; D015342:DNA Probes; D029721:Drosophila Proteins; D014157:Transcription Factors; D004441:Ecdysterone
vernacular_title: Klonirovanie gena BR-C u Drosophila virilis.