CD63, a member of tetraspan transmembrane protein family, induces cellular spreading by reaction with monoclonal antibody on substrata.
In our previous paper, we reported that a monoclonal antibody (MAb) 710F, which recognizes a cell surface glycoprotein, induces morphological change in monocytic cells, promoting cellular adhesion and extensive spreading on tissue culture dishes [Koyama et al., Biochem, Biophys. Res. Commun., 168 (1990) 898-904]. We report here that the antigen was identified to be CD63, a member of tetraspan transmembrane protein family, by purification of the antigen molecule and subsequent cDNA cloning. The identity was further evidenced by sequential immunoprecipitation experiments using MAb 710F and anti-CD63, MAb2.28, and by their reactions with insect cells infected with baculovirus carrying CD63 expression cassette. Furthermore, the MAb2.28 exhibited cell-spreading activity, although to a lesser extent than MAb710F, suggesting the participation of CD63 in adhesion and spreading of monocytic cells.
Koyama
Y
Y
Department of Biochemistry, Hokkaido University School of Medicine, Sapporo, Japan. y_koyama@med.hokudai.ac.jp
Suzuki
M
M
Yoshida
T
T
D000911:Antibodies, Monoclonal; D015703:Antigens, CD / Q000235:genetics / Q000276:immunology / Q000378:metabolism*; D002448:Cell Adhesion; D015815:Cell Adhesion Molecules / Q000235:genetics / Q000276:immunology / Q000378:metabolism*; D048429:Cell Size; D003001:Cloning, Molecular; D018922:HL-60 Cells; D006801:Humans; D009000:Monocytes / Q000166:cytology*; D010980:Platelet Membrane Glycoproteins / Q000235:genetics / Q000276:immunology / Q000378:metabolism*; D011233:Precipitin Tests; D011994:Recombinant Proteins / Q000276:immunology; D017422:Sequence Analysis, DNA; D015398:Signal Transduction; D060149:Tetraspanin 30
D000911:Antibodies, Monoclonal; D015703:Antigens, CD; C554586:CD63 protein, human; D015815:Cell Adhesion Molecules; D010980:Platelet Membrane Glycoproteins; D011994:Recombinant Proteins; D060149:Tetraspanin 30