title: Structural elements of the Streptomyces oriC region and their interactions with the DnaA protein.
abstract: Streptomycetes differ from other prokaryotic organisms in their mycelial life cycle and in possessing a large, linear, GC-rich chromosome. To deduce structural features of the Streptomyces origin of chromosomal replication, the oriC sequences of three Streptomyces species (S. antibioticus, S. chrysomallus and S. lividans) were compared. In Streptomyces, the oriC region contains 19 DnaA boxes whose location, orientation and spacing are conserved. The consensus sequence of the DnaA box identified within Streptomyces oriC is (T/C)(T/C)(G/A/C)TCCACA (preferred bases underlined). The interactions of DnaA with DNA fragments containing single, two or three DnaA boxes were studied using surface plasmon resonance. The dissociation constant (KD) for specific binding of individual DnaA boxes varied between 12 and 78 nM. Streptomyces oriC does not contain the three AT-rich 13-mer direct repeats present in the 5' part of the Escherichia coli oriC region. However, short AT-rich sequences are distributed among the DnaA boxes of Streptomyces oriC. Repeated attempts to unwind Streptomyces oriC have been unsuccessful. It remains to be elucidated whether DnaA interacts with putative accessory proteins which help in unwinding Streptomyces oriC.
authors.affiliation: Ludwik Hirszfeld Institute of Immunology and Experimental Therapy, Polish Academy of Sciences, ul. Weigla 12, 53-114 Wroclaw, Poland.
authors.forename: Dagmara
authors.initials: D
authors.lastname: Jakimowicz
authors.affiliation: Max-Planck-Institut für Molekulare Genetik, Ihnestraße 73, D-14195 Berlin-Dahlem, Germany.|Ludwik Hirszfeld Institute of Immunology and Experimental Therapy, Polish Academy of Sciences, ul. Weigla 12, 53-114 Wroclaw, Poland.
authors.forename: Jerzy
authors.initials: J
authors.lastname: Majka
authors.affiliation: Max-Planck-Institut für Molekulare Genetik, Ihnestraße 73, D-14195 Berlin-Dahlem, Germany.
authors.forename: Walter
authors.initials: W
authors.lastname: Messer
authors.affiliation: Max-Planck-Institut für Molekulare Genetik, Ihnestraße 73, D-14195 Berlin-Dahlem, Germany.
authors.forename: Christian
authors.initials: C
authors.lastname: Speck
authors.affiliation: Departamento de Biologia Funcional e Instituto Universitario de Biotecnologia de Asturias, Universidad de Oviedo, J. Claveria 6, Oviedo 33006, Spain.
authors.forename: Marisol
authors.initials: M
authors.lastname: Fernandez
authors.affiliation: Departamento de Biologia Funcional e Instituto Universitario de Biotecnologia de Asturias, Universidad de Oviedo, J. Claveria 6, Oviedo 33006, Spain.
authors.forename: M
authors.initials: M
authors.lastname: Cruz Martin
authors.affiliation: Departamento de Biologia Funcional e Instituto Universitario de Biotecnologia de Asturias, Universidad de Oviedo, J. Claveria 6, Oviedo 33006, Spain.
authors.forename: Jesus
authors.initials: J
authors.lastname: Sanchez
authors.affiliation: Max-Volmer-Institut für Biophysikalische Chemie und Biochemie, Fachgebiet Biochemie und Molekulare Biologie, Technische Universität Berlin, Franklinstrasse 29, D-10587 Berlin, Germany.
authors.forename: Florian
authors.initials: F
authors.lastname: Schauwecker
authors.affiliation: Max-Volmer-Institut für Biophysikalische Chemie und Biochemie, Fachgebiet Biochemie und Molekulare Biologie, Technische Universität Berlin, Franklinstrasse 29, D-10587 Berlin, Germany.
authors.forename: Ullrich
authors.initials: U
authors.lastname: Keller
authors.affiliation: Fachbereich Biologie/Chemie, Universität Osnabrück, Barbarastraße 11, 49069 Osnabrück, Germany.
authors.forename: Hildgund
authors.initials: H
authors.lastname: Schrempf
authors.affiliation: Ludwik Hirszfeld Institute of Immunology and Experimental Therapy, Polish Academy of Sciences, ul. Weigla 12, 53-114 Wroclaw, Poland.
authors.forename: Jolanta
authors.initials: J
authors.lastname: Zakrzewska-Czerwinńska
mesh_terms: D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism*; D001483:Base Sequence; D003001:Cloning, Molecular; D016384:Consensus Sequence; D004261:DNA Replication; D004268:DNA-Binding Proteins / Q000235:genetics / Q000378:metabolism*; D007700:Kinetics; D008969:Molecular Sequence Data; D012045:Regulatory Sequences, Nucleic Acid; D018741:Replication Origin; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D013302:Streptomyces / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism
chemical_list: D001426:Bacterial Proteins; D004268:DNA-Binding Proteins; C066312:DnaA protein, Bacteria