Rat liver genesine: a biochemical approach.
Genesine, the naked autoglucosylating protein involved in glycogen biosynthesis, was partially purified from rat liver and some of its biochemical properties were characterized. Its activity was strongly activated by Mn2+ and two-pH optimums were obtained. UDP-14C-Glc was the preferred glucosyl donor substrate, but also UDP-14C-Xyl was. It was additionally found that more than one glucose was transferred to the protein or to that alpha1,4 glucan already linked to the protein from UDP-Glc. Glucose, maltose, xylose and UDP were inhibitors of genesine activity.
Lerner
L R
LR
Instituto de Investigaciones Bioquímicas Luis F. Leloir Fundación Campomar, Buenos Aires, Argentina.
Gil
M
M
Krisman
C R
CR
D000818:Animals; D050260:Carbohydrate Metabolism; D002412:Cations; D006023:Glycoproteins / Q000737:chemistry / Q000302:isolation & purification*; D016695:Glycosyltransferases; D006863:Hydrogen-Ion Concentration; D008099:Liver / Q000737:chemistry*; D011509:Proteoglycans / Q000096:biosynthesis; D051381:Rats; D013696:Temperature; D013997:Time Factors; D014532:Uridine Diphosphate Glucose / Q000378:metabolism
D002412:Cations; D006023:Glycoproteins; D011509:Proteoglycans; C113502:genesine protein, rat; D016695:Glycosyltransferases; D014532:Uridine Diphosphate Glucose