Efficacy of ophthalmic solutions to detach adhering Pseudomonas aeruginosa from contact lenses.
OBJECTIVE
To compare the efficacies of two all-in-one contact lens (CL) cleaning solutions and a detergent mixture on the detachment of a pathogenic bacterium adhering to two types of contact lenses in the absence and presence of a tear film.


METHODS
Bacterial-detachment studies were carried out in a parallel-plate flow chamber. Rigid gas permeable (RGP) CLs with and without a tear film were fixed on the bottom plate of the flow chamber. After adhesion of Pseudomonas aeruginosa no. 3, bacterial detachment was stimulated by perfusing the system either with an all-in-one CL-cleaning solution, for soft contact lenses (SCL solution) and for rigid lenses (RCL solution), or with a detergent mixture of 0.25% (wt/vol) sodium lauryl sulfate (SLS) and 0.2% sodium methyl cocoyl taurate (Tauranol). In addition, the all-in-one RCL-cleaning solution supplemented with 0.025% (wt/vol) SLS and 0.02% (wt/vol) Tauranol was evaluated. A surface physical-chemical analysis of the lenses before and after application of the solutions was done to determine whether remnants of the ophthalmic solutions or detergents could be found adsorbed to the CL surfaces.


RESULTS
Both all-in-one CL-cleaning solutions stimulated minor bacterial detachment from CL surfaces with or without a tear film. The SLS/Tauranol detergent mixture, however, removed < or = 95% of the adhering P. aeruginosa cells, whereas the RCL-cleaning solution supplemented with detergents also stimulated significant detachment. Surface physical-chemical analysis clearly demonstrated the presence of a tear film on the CL surfaces, but remnants neither of the ophthalmic solutions nor of the detergents could be found.


CONCLUSIONS
Ophthalmic solutions are not effective in stimulating detachment of adhering bacteria from CL surfaces. Supplementing of an all-in-one CL-cleaning solution with only small amounts of detergents yielded a solution much more effective in stimulating bacterial detachment while leaving no detectable remnants of the ophthalmic solution or of the detergents on the CL surfaces.
Landa
A S
AS
Laboratory for Materia Technica, University Hospital Groningen, The Netherlands.
van der Mei
H C
HC
van Rij
G
G
Busscher
H J
HJ
D001422:Bacterial Adhesion / Q000187:drug effects*; D015169:Colony Count, Microbial; D018639:Contact Lens Solutions / Q000737:chemistry / Q000494:pharmacology*; D003261:Contact Lenses / Q000382:microbiology*; D004338:Drug Combinations; D015818:Eye Infections, Bacterial / Q000517:prevention & control; D006801:Humans; D011550:Pseudomonas aeruginosa / Q000187:drug effects / Q000302:isolation & purification / Q000502:physiology*; D013666:Tears / Q000382:microbiology; D016896:Treatment Outcome
D018639:Contact Lens Solutions; D004338:Drug Combinations