title: Modulation of the activation status of Stat5a during LIF-induced differentiation of M1 myeloid leukemia cells.
abstract: Treatment of M1 myeloid leukemia cells with leukemia inhibitory factor (LIF) causes activation of transcription factors Stat1, Stat3 and Stat5a (signal transducers and activators of transcription). DNA-binding of Stat proteins was detectable for extended periods of time in LIF-treated M1 cells, which simultaneously underwent terminal differentiation. The relative composition of Stat factors in the protein-DNA complexes changed during time. Whereas Stat3 was activated up to 36 h during treatment with LIF, Stat5a was activated only short-termed. Similarly, high expression of the immediate early gene CIS (cytokine-inducible SH2-containing protein), a known target gene of Stat5 in hematopoietic cells, occurred only during the onset of differentiation. This suggests a role of Stat5a in the early phase of LIF-induced differentiation and growth arrest of M1 cells.
authors.affiliation: Institut fúr Mikrobiologie, Biochemie und Genetik, University of Erlangen-Nuernberg, Germany.
authors.forename: R P
authors.initials: RP
authors.lastname: Piekorz
authors.forename: R
authors.initials: R
authors.lastname: Rinke
authors.forename: F
authors.initials: F
authors.lastname: Gouilleux
authors.forename: B
authors.initials: B
authors.lastname: Neumann
authors.forename: B
authors.initials: B
authors.lastname: Groner
authors.forename: G M
authors.initials: GM
authors.lastname: Hocke
mesh_terms: D000818:Animals; D016466:CHO Cells; D002454:Cell Differentiation / Q000187:drug effects; D006224:Cricetinae; D004268:DNA-Binding Proteins / Q000187:drug effects / Q000378:metabolism*; D005786:Gene Expression Regulation / Q000187:drug effects; D017781:Genes, Immediate-Early / Q000187:drug effects; D006131:Growth Inhibitors / Q000494:pharmacology*; D006801:Humans; D017874:Immediate-Early Proteins / Q000187:drug effects / Q000235:genetics; D015850:Interleukin-6; D007700:Kinetics; D053605:Leukemia Inhibitory Factor; D007951:Leukemia, Myeloid / Q000378:metabolism* / Q000473:pathology*; D008222:Lymphokines / Q000494:pharmacology*; D051379:Mice; D008894:Milk Proteins; D011485:Protein Binding / Q000187:drug effects; D012333:RNA, Messenger / Q000187:drug effects / Q000378:metabolism; D050796:STAT3 Transcription Factor; D050799:STAT5 Transcription Factor; D050826:Suppressor of Cytokine Signaling Proteins; D013997:Time Factors; D015534:Trans-Activators / Q000187:drug effects / Q000378:metabolism*; D014407:Tumor Cells, Cultured; D025521:Tumor Suppressor Proteins
chemical_list: D004268:DNA-Binding Proteins; D006131:Growth Inhibitors; D017874:Immediate-Early Proteins; D015850:Interleukin-6; D053605:Leukemia Inhibitory Factor; D008222:Lymphokines; D008894:Milk Proteins; D012333:RNA, Messenger; D050796:STAT3 Transcription Factor; D050799:STAT5 Transcription Factor; D050826:Suppressor of Cytokine Signaling Proteins; D015534:Trans-Activators; D025521:Tumor Suppressor Proteins; C507837:LIF protein, human; C507838:Lif protein, mouse; C494086:STAT3 protein, human; C494092:STAT5A protein, human; C494087:Stat3 protein, mouse; C494093:Stat5a protein, mouse