The impact of donor organ quality on postoperative liver function after orthotopic rat liver transplantation.
The impact of donor factors for posttransplant liver function was evaluated in the model of orthotopic rearterialized liver transplantation in the rat. The effect of donor fasting, parenteral hyperalimentation, hypotension, warm ischemia and endotoxins on histology, clinical chemistry and MEGX test was analyzed in syngeneic and allogeneic recipients of livers stored for 4 hrs on ice. In syngeneic animals, 20 min of warm ischemia led to significantly elevated serum transaminase levels and degree 2 histological damage on POD 2. Endotoxins produced a grade 1 histological damage. All groups had a lower MEGX formation rate compared to controls. In allogeneic animals, warm ischemia was the single most detrimental parameter. The strength of the rejection response on POD 8 did not depend on the type of donor pretreatment. The major finding of this non-survival study is the deleterious effect of warm ischemia and endotoxin on the functional and structural integrity of liver grafts after 4 hrs of cold ischemia.
Knoop
M
M
Chirurgische Klinik und Poliklinik, Virchow Klinikum, Humboldt Universität, Berlin, Germany.
Neumann
U
U
Lang
M
M
Neuhaus
P
P
D000818:Animals; D001810:Blood Volume / Q000502:physiology; D004734:Energy Metabolism / Q000502:physiology; D004926:Escherichia coli / Q000276:immunology; D008070:Lipopolysaccharides / Q000276:immunology; D008099:Liver / Q000473:pathology; D008111:Liver Function Tests; D016031:Liver Transplantation / Q000473:pathology / Q000502:physiology*; D008297:Male; D009926:Organ Preservation; D051381:Rats; D011917:Rats, Inbred Lew; D011919:Rats, Inbred Strains; D014019:Tissue Donors; D014022:Tissue Survival / Q000502:physiology; D014184:Transplantation, Homologous; D014185:Transplantation, Isogeneic
D008070:Lipopolysaccharides