[Polypeptide composition of major antigens and identification of prevalent immunogenic proteins of opisthorchis felineus].
The polypeptide composition of somatic, excretory-secretory, and egg antigens of the liver trematode Opisthorchis felineus has been investigated by SDS electrophoresis. All three main antigens consist of a large number of different polypeptides and have original electrophoretic profiles. The first original classification of the main polypeptides of somatic, excretory-secretory, and egg antigens of O. felineus is proposed. Immunoblotting showed that serum antibodies fail to recognize all polypeptides. The human serum antibodies mainly interacted with protein p105 alone. The restricted human immune response can be critical to further development of immunoassay for opisthorchiasis. Eighteen monoclonal antibodies against O. felineus p105 were obtained and their main properties were examined. Furthermore, this will improve immunoassay and investigation of the mechanisms responsible for immune response to O. felineus antigens.
Kotelkin
A T
AT
Razumov
I A
IA
Loktev
V B
VB
D000818:Animals; D000909:Antibodies, Helminth / Q000276:immunology; D000947:Antigens, Helminth / Q000737:chemistry*; D004591:Electrophoresis, Polyacrylamide Gel; D015801:Helminth Proteins / Q000276:immunology*; D006801:Humans; D007118:Immunoassay; D008099:Liver / Q000469:parasitology; D051379:Mice; D008807:Mice, Inbred BALB C; D009889:Opisthorchiasis / Q000276:immunology / Q000469:parasitology; D009891:Opisthorchis / Q000276:immunology*; D010455:Peptides / Q000032:analysis*; D011817:Rabbits
D000909:Antibodies, Helminth; D000947:Antigens, Helminth; D015801:Helminth Proteins; D010455:Peptides
Polipeptidnyĭ sostav osnovnykh antigenov i vyiavlenie dominiruiushchikh immunogennykh belkov opisthorchis felineus.