Modulation of the activation status of Stat5a during LIF-induced differentiation of M1 myeloid leukemia cells.
Treatment of M1 myeloid leukemia cells with leukemia inhibitory factor (LIF) causes activation of transcription factors Stat1, Stat3 and Stat5a (signal transducers and activators of transcription). DNA-binding of Stat proteins was detectable for extended periods of time in LIF-treated M1 cells, which simultaneously underwent terminal differentiation. The relative composition of Stat factors in the protein-DNA complexes changed during time. Whereas Stat3 was activated up to 36 h during treatment with LIF, Stat5a was activated only short-termed. Similarly, high expression of the immediate early gene CIS (cytokine-inducible SH2-containing protein), a known target gene of Stat5 in hematopoietic cells, occurred only during the onset of differentiation. This suggests a role of Stat5a in the early phase of LIF-induced differentiation and growth arrest of M1 cells.
Piekorz
R P
RP
Institut fúr Mikrobiologie, Biochemie und Genetik, University of Erlangen-Nuernberg, Germany.
Rinke
R
R
Gouilleux
F
F
Neumann
B
B
Groner
B
B
Hocke
G M
GM
D000818:Animals; D016466:CHO Cells; D002454:Cell Differentiation / Q000187:drug effects; D006224:Cricetinae; D004268:DNA-Binding Proteins / Q000187:drug effects / Q000378:metabolism*; D005786:Gene Expression Regulation / Q000187:drug effects; D017781:Genes, Immediate-Early / Q000187:drug effects; D006131:Growth Inhibitors / Q000494:pharmacology*; D006801:Humans; D017874:Immediate-Early Proteins / Q000187:drug effects / Q000235:genetics; D015850:Interleukin-6; D007700:Kinetics; D053605:Leukemia Inhibitory Factor; D007951:Leukemia, Myeloid / Q000378:metabolism* / Q000473:pathology*; D008222:Lymphokines / Q000494:pharmacology*; D051379:Mice; D008894:Milk Proteins; D011485:Protein Binding / Q000187:drug effects; D012333:RNA, Messenger / Q000187:drug effects / Q000378:metabolism; D050796:STAT3 Transcription Factor; D050799:STAT5 Transcription Factor; D050826:Suppressor of Cytokine Signaling Proteins; D013997:Time Factors; D015534:Trans-Activators / Q000187:drug effects / Q000378:metabolism*; D014407:Tumor Cells, Cultured; D025521:Tumor Suppressor Proteins
D004268:DNA-Binding Proteins; D006131:Growth Inhibitors; D017874:Immediate-Early Proteins; D015850:Interleukin-6; D053605:Leukemia Inhibitory Factor; D008222:Lymphokines; D008894:Milk Proteins; D012333:RNA, Messenger; D050796:STAT3 Transcription Factor; D050799:STAT5 Transcription Factor; D050826:Suppressor of Cytokine Signaling Proteins; D015534:Trans-Activators; D025521:Tumor Suppressor Proteins; C507837:LIF protein, human; C507838:Lif protein, mouse; C494086:STAT3 protein, human; C494092:STAT5A protein, human; C494087:Stat3 protein, mouse; C494093:Stat5a protein, mouse