Expression of costimulatory molecule CD80 on peripheral blood T cells in patients with systemic lupus erythematosus.
OBJECTIVE
To investigate the expression of costimulatory molecule CD80 on T cells of peripheral blood mononuclear cells (PBMC) in patients with systemic lupus erythematosus (SLE).


METHODS
Monoclonal antibodies against CD80 were used for flow cytometry and expression of CD80 on PBMC was studied in 26 patients with SLE, 18 patients with rheumatoid arthritis (RA), 8 patients with mixed connective tissue disease (MCTD), and 22 healthy controls.


RESULTS
CD80 was detected on CD3+ and CD19+ cells in patients with SLE and it was significantly higher than that of controls. In patients with SLE CD80 was expressed on CD4+ T cells (8.05+/-5.45%), significantly higher than in RA and controls, but was not highly expressed on CD8+ T cells (1.67+/-2.87%). CD80+CD4+ T cell phenotype analysis revealed CD45RA-, CD45RO+, and CD25+, or HLA-DR+ activated T cells. The percentage of CD80+ cells in CD4+ cells increased in the active stage of SLE, and was significantly correlated with the SLE disease activity index.


CONCLUSIONS
CD80 can be expressed on activated CD4+ T cells in PBMC of patients with SLE in vivo and the appearance of these cells is associated with the disease activity in SLE.
Takasaki
Y
Y
Department of Internal Medicine and Rheumatology, Juntendo University School of Medicine, Mitsubishi Chemical Corporation, Tokyo, Japan.
Ogaki
M
M
Abe
K
K
Takeuchi
K
K
Ando
S
S
Tokano
Y
Y
Kobayashi
S
S
Sekigawa
I
I
Tsuda
H
H
Hashimoto
H
H
D000911:Antibodies, Monoclonal / Q000032:analysis; D015703:Antigens, CD / Q000378:metabolism; D001172:Arthritis, Rheumatoid / Q000097:blood; D018122:B7-1 Antigen / Q000378:metabolism*; D015496:CD4-Positive T-Lymphocytes / Q000378:metabolism*; D018414:CD8-Positive T-Lymphocytes / Q000378:metabolism*; D005260:Female; D005434:Flow Cytometry; D005455:Fluorescent Antibody Technique; D006801:Humans; D008180:Lupus Erythematosus, Systemic / Q000097:blood*; D008297:Male; D008875:Middle Aged; D009000:Monocytes / Q000378:metabolism; D010641:Phenotype; D012720:Severity of Illness Index
D000911:Antibodies, Monoclonal; D015703:Antigens, CD; D018122:B7-1 Antigen