The Arcanobacterium (Actinomyces) pyogenes plasmid pAP1 is a member of the pIJ101/pJV1 family of rolling circle replication plasmids.
The 2.4-kb plasmid pAP1 from Arcanobacterium (Actinomyces) pyogenes had sequence similarity within the putative replication protein and double-stranded origin with the pIJ101/pJV1 family of plasmids. pJGS84, a derivative of pAP1 containing a kanamycin resistance gene, was able to replicate in Escherichia coli and Corynebacterium pseudotuberculosis, as well as in A. pyogenes. Detection of single-stranded DNA intermediates of pJGS84 replication suggested that this plasmid replicates by the rolling circle mechanism.
Billington
S J
SJ
Department of Veterinary Science and Microbiology, University of Arizona, Tucson 85721, USA. billing@vetsci.microvet.arizona.edu
Jost
B H
BH
Songer
J G
JG
D000190:Actinomyces / Q000235:genetics* / Q000378:metabolism; D000595:Amino Acid Sequence; D001483:Base Sequence; D003001:Cloning, Molecular; D004265:DNA Helicases / Q000235:genetics; D004261:DNA Replication; D004268:DNA-Binding Proteins; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation; D000073718:Pancreatitis-Associated Proteins; D010957:Plasmids / Q000737:chemistry / Q000235:genetics*; D018741:Replication Origin; D017386:Sequence Homology, Amino Acid; D012689:Sequence Homology, Nucleic Acid; D015534:Trans-Activators / Q000235:genetics
D004268:DNA-Binding Proteins; D000073718:Pancreatitis-Associated Proteins; C000612141:REG3A protein, human; D015534:Trans-Activators; C045264:replication initiator protein; D004265:DNA Helicases