title: Isolation and characterization of a functional A-type cyclin from maize.
abstract: Cyclins are involved in the regulation of cell cycle progression in eukaryotes. We have isolated a cyclin cDNA clone, cycZm2w, from maize root tip cells, which fits best into group A2 of current plant cyclin gene classification schemes. The cDNA encodes a protein with a domain homologous to the cyclin box of mitotic cyclins. Complementation studies revealed that cycZm2w was able to rescue a budding yeast cyclin-deficient mutant (BF305-15d#21). As expected, cycZm2w is expressed in organs of the maize plant that possess meristematic activity, but is especially prominent in the proliferating regions of the root apex.
authors.affiliation: University of Maryland, Department of Plant Biology, College Park 20742, USA.
authors.forename: W L
authors.initials: WL
authors.lastname: Hsieh
authors.forename: S M
authors.initials: SM
authors.lastname: Wolniak
mesh_terms: D019925:Cyclin A / Q000235:genetics* / Q000378:metabolism; D018076:DNA, Complementary / Q000235:genetics; D004351:Drug Resistance; D015870:Gene Expression; D015723:Gene Library; D005816:Genetic Complementation Test; D000072235:Mating Factor; D008969:Molecular Sequence Data; D010455:Peptides / Q000494:pharmacology; D010940:Plant Proteins / Q000235:genetics; D018517:Plant Roots / Q000235:genetics; D011994:Recombinant Proteins / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000235:genetics; D017422:Sequence Analysis, DNA; D014018:Tissue Distribution; D003313:Zea mays / Q000235:genetics*
chemical_list: D019925:Cyclin A; D018076:DNA, Complementary; D010455:Peptides; D010940:Plant Proteins; D011994:Recombinant Proteins; D000072235:Mating Factor