Rat urinary chemiluminescence: effect of ethanol and/or hexachlorobenzene uptake.
Hexachlorobenzene (HCB) administration to rats induces porphyria cutanea tarda, characterized by high levels of urinary porphyrins (> 40 micrograms/day) and accumulation of highly carboxylated porphyrins in liver (> 15 micrograms/g of tissue). Ethanol administration, under the conditions employed, was not porphyrinogenic and was able to diminish some of the responses elicited by HCB. Furthermore, ethanol and/or HCB administration leads to organ disturbances that involve oxidative stress. We have measured the changes in urinary chemiluminescence (CL) levels, as part of a systematic evaluation of the metabolic alterations in rats chronically treated with ethanol and/or HCB. The results, that constitute the first set of urinary CL data obtained from an animal model system, indicate that the measurement of the spontaneous urinary CL can constitute a fast, simple and sensitive method to evaluate disturbances associated with oxidative stress.
Ríos de Molina
M C
MC
Departmento de Química Biológica, Facultad de Ciencias Exactas Y Naturales, Universidad de Buenos Aires, Argentina.
Suárez Lissi
A M
AM
Armesto
A
A
Lafourcade
C
C
Lissi
E
E
D000818:Animals; D000431:Ethanol / Q000633:toxicity*; D006581:Hexachlorobenzene / Q000633:toxicity*; D008099:Liver / Q000187:drug effects / Q000378:metabolism; D008163:Luminescent Measurements; D018384:Oxidative Stress / Q000187:drug effects; D017119:Porphyria Cutanea Tarda / Q000139:chemically induced / Q000378:metabolism / Q000652:urine; D011166:Porphyrins / Q000652:urine; D051381:Rats; D017208:Rats, Wistar; D014556:Urine / Q000737:chemistry*
D011166:Porphyrins; D000431:Ethanol; D006581:Hexachlorobenzene