[Role of CAAT/enhancer binding protein in IL-6 regulation of angiotensinogen gene expression].
OBJECTIVE
Angiotensinogen is the only known precursor of vasoactive angiotensin II and also one of the acute phase proteins. This study was intended to understand the regulation of angiotensinogen gene expression induced by IL-6.


METHODS
Northern hybridization, electrophoretic mobility shift assay and transient transfections were conducted.


RESULTS
Northern hybridization showed increase of angiotensinogen mRNA treated by IL-6 in Hep3B cells. Electrophoretic mobility shift assay further indicated the HAG IL-6RE homologous to IL-6 responsive element at -568 site of the angiotensinogen promoter binds C/EBP(CAAT/Enhancer Binding Protein). Consistent with this binding studies were the transient transfections of the expression vector in which 6 copies of HAG-IL-6RE linked to TK core promoter and fused to CAT reporter gene, revealing that C/EBPalpha was a transactivator under IL-6-induced condition.


CONCLUSIONS
These observations suggest that C/EBP plays regulatory role in IL-6-induced angiotensinogen gene expression.
Zhao
Y
Y
Department of Medical Genetics, China Medical University, Shenyang, Liaoning Province, 110001 P.R.China. yyzhao@iris.cmu.edu.cn
Shen
K
K
D000808:Angiotensinogen / Q000235:genetics*; D022762:CCAAT-Enhancer-Binding Proteins; D004268:DNA-Binding Proteins / Q000502:physiology*; D005786:Gene Expression Regulation / Q000187:drug effects*; D006801:Humans; D015850:Interleukin-6 / Q000494:pharmacology*; D009687:Nuclear Proteins / Q000502:physiology*
D022762:CCAAT-Enhancer-Binding Proteins; D004268:DNA-Binding Proteins; D015850:Interleukin-6; D009687:Nuclear Proteins; D000808:Angiotensinogen