title: Refined genetic map of the obligate methylotroph Methylobacillus flagellatum.
abstract: We present a refined genetic map of the obligate methylotroph Methylobacillus flagellatum. New, Hfr (high-frequency-of-transfer) donors, and pulsed-field gel electrophoresis, were used to determine that M. flagellatum contains one approximately 3.1-Mb circular chromosome, and no plasmids. A correlation between time-of-entry units and DNA length was established. Using in vivo and in vitro cloning, and sequencing, a number of new genetic markers were identified and mapped; in addition, the nature of some of the previously mapped markers was elucidated.
authors.affiliation: Institute of Genetics and Selection of Industrial Microorganisms, Moscow, Russia.
authors.forename: I
authors.initials: I
authors.lastname: Serebrijski
authors.forename: M
authors.initials: M
authors.lastname: Gomelsky
authors.forename: E
authors.initials: E
authors.lastname: Bashkirova
authors.forename: A
authors.initials: A
authors.lastname: Chistoserdov
authors.forename: Y D
authors.initials: YD
authors.lastname: Tsygankov
mesh_terms: D002874:Chromosome Mapping; D002876:Chromosomes, Bacterial; D003001:Cloning, Molecular; D005798:Genes, Bacterial; D005819:Genetic Markers; D006089:Gram-Negative Anaerobic Bacteria / Q000235:genetics*; D000637:Transaminases / Q000235:genetics
chemical_list: D005819:Genetic Markers; D000637:Transaminases; C053430:histidinol-phosphate aminotransferase