title: Production of L-malic acid via biocatalysis employing wild-type and respiratory-deficient yeasts.
abstract: The yeast Saccharomyces cerevisiae has been used to efficiently produce L-malic acid from fumaric acid. Fumarase is responsible for the reversible conversion of fumaric and L-malic acids in the TCA cycle. To investigate the function of mitochondrial and cytoplasmic fumarase isoenzymes in L-malic acid bioconversion, a wild-type strain and a cytoplasmic respiratory-deficient mutant devoid of functional mitochondria were employed. The mutant strain, which only contained the cytoplasmic fumarase, was still functional in fumaric acid to L-malic acid bioconversion However, its specific conversion rate was much lower (0.20 g/g.h) than that of the wild-type strain (0.55 g/g.h).
authors.affiliation: Laboratory of Renewable Resources Engineering, Potter Engineering Center, Purdue University, West Lafayette, IN 47907, USA.
authors.forename: X
authors.initials: X
authors.lastname: Wang
authors.forename: C S
authors.initials: CS
authors.lastname: Gong
authors.forename: G T
authors.initials: GT
authors.lastname: Tsao
mesh_terms: D002384:Catalysis; D004396:Coloring Agents; D003593:Cytoplasm / Q000201:enzymology; D005285:Fermentation; D005649:Fumarate Hydratase / Q000737:chemistry; D005650:Fumarates / Q000737:chemistry; D008293:Malates / Q000138:chemical synthesis*; D008928:Mitochondria / Q000201:enzymology; D010101:Oxygen Consumption / Q000235:genetics; D012441:Saccharomyces cerevisiae / Q000235:genetics / Q000378:metabolism*; D013237:Stereoisomerism; D013778:Tetrazolium Salts
chemical_list: D004396:Coloring Agents; D005650:Fumarates; D008293:Malates; D013778:Tetrazolium Salts; C009591:triphenyltetrazolium; C030298:malic acid; C032005:fumaric acid; D005649:Fumarate Hydratase