title: Neuron number in the myenteric plexus of the ascending colon of rats. A comparative study using two staining techniques.
abstract: We carried out this study with the purpose of comparing the neuronal density in antimesocolic and intermediate regions of the colon of rats. We used the ascending colon of ten seven-months old Wistar rats. With the Giemsa method we found 29,046 neurons/cm2 on the antimesocolic region and 30,968 neurons/cm2 on the intermediate regions. With the NADH-diaphorase technique 12,308 neurons/cm2 on the antimesocolic regions and 8798 neurons/cm2 on the intermediate regions were evidenced. The number of NADH-diaphorase positive neurons is significantly less than the number of Giemsa-stained neurons and that this difference is enhanced on the intermediate regions of the intestinal circumference. Therefore, to compare the number of neurons of an intestinal segment of a same species at the same age, it is necessary to take into consideration the technique employed and the region of the intestinal circumference from where the sample was obtained.
authors.affiliation: Departamento de Ciéncias Morfofísiológicas, Universidade Estadual de Maringá, Brasil.
authors.forename: D M
authors.initials: DM
authors.lastname: Sant'Ana
authors.forename: M H
authors.initials: MH
authors.lastname: Miranda-Neto
authors.forename: S L
authors.initials: SL
authors.lastname: Molinari
authors.forename: M A
authors.initials: MA
authors.lastname: Sant'Anna
mesh_terms: D000818:Animals; D001399:Azure Stains; D002452:Cell Count; D003106:Colon / Q000166:cytology*; D008058:Dihydrolipoamide Dehydrogenase; D008297:Male; D009197:Myenteric Plexus / Q000166:cytology*; D051381:Rats; D017208:Rats, Wistar
chemical_list: D001399:Azure Stains; D008058:Dihydrolipoamide Dehydrogenase