Plasma amylin immunoreactivity and insulin resistance in insulin resistant relatives of patients with non-insulin-dependent diabetes mellitus.
To explore the potential relationship between concentrations of circulating amylin and the insulin resistance observed in first-degree relatives of patients with non-insulin-dependent-diabetes mellitus (NIDDM), we studied 40 relatives compared to 35 matched controls. Two newly developed immunoassays that measure either non-glycosylated or total amylin were applied. All subjects were examined by an oral glucose tolerance test (OGTT) and by a hyperinsulinemic euglycemic clamp (insulin infusion: 0.6 mU/kg/min). Glucose tolerance was normal in all, but insulin-stimulated glucose uptake (Rd) was diminished in the relatives (p < 0.001). Area under the curves (AUCs) during OGTT for plasma glucose (p < 0.01) and serum insulin (p=0.08), but not for plasma total and non-glycosylated amylin, were higher in relatives versus controls. In both groups, inverse correlations were found between Rd and AUC for plasma total and non-glycosylated amylin (p [all]<0.05). However, in multiple linear regression analyses, plasma total and non-glycosylated amylin failed to influence Rd independent of serum insulin and family history-of NIDDM. In conclusion, this study demonstrated inverse correlations between Rd and circulating concentrations of plasma total and non-glycosylated amylin in relatives and matched controls. These data, however, do not support the hypothesis that physiological amylin concentration are a major importance for the insulin resistance in relatives of NIDDM patients.
Nyholm
B
B
Department of Medicine M (Endocrinology and Diabetes), Aarhus Kommunehospital, University Hospital of Aarhus, Denmark.
Fineman
M S
MS
Koda
J E
JE
Schmitz
O
O
D000328:Adult; D000682:Amyloid / Q000097:blood*; D001786:Blood Glucose / Q000378:metabolism; D015992:Body Mass Index; D016022:Case-Control Studies; D003924:Diabetes Mellitus, Type 2 / Q000097:blood / Q000235:genetics*; D005260:Female; D005951:Glucose Tolerance Test; D006801:Humans; D007118:Immunoassay; D007328:Insulin / Q000097:blood; D007333:Insulin Resistance / Q000235:genetics*; D058228:Islet Amyloid Polypeptide; D016014:Linear Models; D008297:Male; D008875:Middle Aged
D000682:Amyloid; D001786:Blood Glucose; D007328:Insulin; D058228:Islet Amyloid Polypeptide