title: Comparison of wound healing process using Argon and Krypton lasers.
abstract: OBJECTIVE
  We compared the wound healing process on Sprague-Dawley rats between Argon Laser and Krypton Laser, and calculated the laser actual doses after correction on wound healing on rats and estimated the clinical doses on wound healing for human skin.
  
  
  BACKGROUND
  Laboratory work provided some support for the use of low-intensity laser radiation in wound healing. Some studies found that laser irradiation may either enhance, inhibit, or has no effect on the function of a variety of microorganisms and cells. Animal studies also offered some basis for treatment. Improvements, particularly in the earliest phases of wound healing, have been reported following laser irradiation.
  
  
  METHODS
  We used Argon Laser (488-514 nm) and Krypton Laser (670 nm) in the study. The laser beam was delivered through a system of fiber optic in Argon Laser and reflector in Krypton Laser. The rats treated were restrained in a Plexiglas cage without anesthesia during the laser irradiation period. The percentage of the wound healing acceleration in days and size, actual doses, and estimated clinical doses were calculated as follows: AccD = (1-TD/CD) x 100%, AccS = (1-TA/CA) x 100%, AD = D(in) - D(gl) - D(SR) and ECD = AD = D(sr), respectively.
  
  
  RESULTS
  The acceleration effects of wound healing in days (AccD) were 22.93% and 14.54%, size reductions (AccS) were 41.93% and 30.41% at the optimal stimulative incident dose of 20 J/cm2. Zero bioactivation shown at the incident doses of 80 J/cm2 and 100 J/cm2. The inhibitory effects of wound healing in days were -7.72% and -3.37%, in size reduction were -13.35% and -12.88% at the maximal inhibitory incident dose of 140 J/cm2 for Argon and Krypton Lasers, respectively. The actual doses were 5.21 J/cm2 and 4.03 J/cm2, the estimated clinical doses were 5.50 J/cm2 and 4.25 J/cm2 at optimal stimulative incident dose 20 J/cm2 for Argon and Krypton Lasers, respectively.
  
  
  CONCLUSIONS
  Low power laser therapy at the appropriate dosimetric parameters can provide the acceleration effects of wound healing on rats. The effects were dependent with the doses and laser wavelengths used. In this experiment, the optimum stimulative dose was 20 J/cm2 and the Argon Laser with 488 nm was more effective than the Krypton Laser with 670 nm. The zero bioactivation and inhibition effect of wound healing on rats occurred in Argon Laser and Krypton Laser.
authors.affiliation: Laser Research, Biological, & Medical Research Department, King Faisal Specialist Hospital and Research Center, Riyadh, Kingdom of Saudi Arabia.
authors.forename: F A
authors.initials: FA
authors.lastname: Al-Watban
authors.forename: X Y
authors.initials: XY
authors.lastname: Zhang
mesh_terms: D000818:Animals; D001128:Argon; D004307:Dose-Response Relationship, Radiation; D005347:Fibroblasts / Q000528:radiation effects; D006801:Humans; D007726:Krypton; D053685:Laser Therapy; D051381:Rats; D017207:Rats, Sprague-Dawley; D012867:Skin / Q000528:radiation effects; D013997:Time Factors; D014945:Wound Healing / Q000528:radiation effects*
chemical_list: D007726:Krypton; D001128:Argon