Adult X-linked immunodeficiency (XID) mice, IGM-/- single knockout and IGM-/- CD8-/- double knockout mice do not clear polyomavirus infection.
The importance of antibodies for elimination of polyomavirus infection and the prevention of virus induced oncogenesis was studied, X-linked immunodeficiency (XID) mice, IgM-/- single knockout and IgMI-/- CD8-/- double knockout mice, all defective in antibody production, and normal control mice were infected with polyomavirus as adults. The mice were followed for presence of polyoma DNA with a polyoma specific polymerase chain reaction (PCR) over 6 weeks post infection (p.i.), a time point at which polyomavirus DNA is no longer detected in normal adult infected mice. As expected, virus DNA was not detected in normal mice 6 weeks p.i. In both IgM-/- single knockout and IgM-/- CD8-/- double knockout mice a disseminated infection was still observed by 6 weeks p.i. and the latter group of mice succumbed around two months p.i. In XID mice, only one third of the mice were still positive for viral DNA 6 weeks p.i. No polyomavirus induced tumors were observed in any of the mice during the 2-4 month observation period.
Berke
Z
Z
Department of Immunology, Microbiology, Pathology and Infectious Diseases, Karolinska Institutet, Stockholm, Sweden.
Mellin
H
H
Heidari
S
S
Wen
T
T
Berglöf
A
A
Klein
G
G
Dalianis
T
T
D000818:Animals; D000914:Antibodies, Viral / Q000097:blood; D016827:CD8 Antigens / Q000235:genetics*; D004279:DNA, Viral / Q000032:analysis; D005260:Female; D008040:Genetic Linkage; D006385:Hemagglutination Inhibition Tests; D007075:Immunoglobulin M / Q000172:deficiency* / Q000235:genetics* / Q000502:physiology; D007153:Immunologic Deficiency Syndromes / Q000235:genetics / Q000276:immunology*; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D008808:Mice, Inbred CBA; D018345:Mice, Knockout; D011120:Polyomavirus / Q000235:genetics / Q000276:immunology*; D027601:Polyomavirus Infections / Q000235:genetics / Q000276:immunology*; D014412:Tumor Virus Infections / Q000235:genetics / Q000276:immunology*; D014960:X Chromosome / Q000276:immunology
D000914:Antibodies, Viral; D016827:CD8 Antigens; D004279:DNA, Viral; D007075:Immunoglobulin M