Variations in gene expression and genomic stability of human hepatoma cells integrated with hepatitis B virus DNA.
The association of hepatitis B virus (HBV) infection with human hepatoma is well established. However, no consensus regarding the etiology of hepatocellular carcinoma was elucidated. In this paper, the genomic stability and gene expression of HBV DNA-integrated and non-integrated hepatoma cells by Transcript Profile (TP)-PCR and RT-PCR are characterized. The additional DNA bands generated from TP-PCR of HBV integrated genomes were not correlated with the sequence of HBV, suggesting that the variations may result from genomic instability of the host cells. Moreover, differential genes expressed in HBV DNA-integrated cells were sequenced. A cDNA generated from the integrated cells exhibited 99.3% homology with the sequences of ATP synthase 6 and cytochrome C oxidase III, but the sequences were abnormally linked together. Since HBV infection may alter the energy metabolism of the cell, the results suggest that the integration may cause mitochondriae defects in the ATP synthase 6 and cytochrome C oxidase III genes.
Kuo
K W
KW
Department of Biochemistry, Kaohsiung Medical College, Taiwan.
Yang
P Y
PY
Huang
Y S
YS
Shieh
D Z
DZ
D025181:ATP Synthetase Complexes; D001483:Base Sequence; D006528:Carcinoma, Hepatocellular / Q000235:genetics* / Q000378:metabolism*; D002471:Cell Transformation, Neoplastic / Q000235:genetics; D004272:DNA, Mitochondrial / Q000235:genetics; D004273:DNA, Neoplasm / Q000235:genetics* / Q000378:metabolism; D004279:DNA, Viral / Q000235:genetics* / Q000378:metabolism; D003576:Electron Transport Complex IV / Q000235:genetics; D015870:Gene Expression; D015894:Genome, Human; D006515:Hepatitis B virus / Q000235:genetics*; D006801:Humans; D008113:Liver Neoplasms / Q000235:genetics* / Q000378:metabolism*; D008969:Molecular Sequence Data; D009097:Multienzyme Complexes / Q000235:genetics; D017856:Phosphotransferases (Phosphate Group Acceptor) / Q000235:genetics; D016133:Polymerase Chain Reaction; D014407:Tumor Cells, Cultured
D004272:DNA, Mitochondrial; D004273:DNA, Neoplasm; D004279:DNA, Viral; D009097:Multienzyme Complexes; D003576:Electron Transport Complex IV; D025181:ATP Synthetase Complexes; D017856:Phosphotransferases (Phosphate Group Acceptor)