title: Long-term antibodies after an oral immunization with cholera toxin are synthesized in the bone marrow and may play a role in the regulation of memory B-cell maintenance at systemic and mucosal sites.
abstract: To study the importance of the bone marrow in the long-term antibody response, IgG and IgA antitoxin antibody-forming cells were evaluated by ELISPOT in Peyer's patches, mesenteric lymph nodes, spleen, lamina propria of the small intestine and bone marrow at several times after oral immunization with cholera toxin. The mesenteric lymph node was the site having the major frequency of IgG antitoxin during the first two weeks after priming, whereas lamina propria was the site with a major number of IgA antitoxin antibody-forming cells. However, from 3 weeks until 10 months after priming, bone marrow became the site with the major frequency of IgG, and especially IgA antitoxin antibody-forming cells (without taking into account the lamina propria). This result indicates that bone marrow was responsible for the long-term antibody response and raises questions concerning the mechanisms involved in the maintenance of antibody production. The importance of bone marrow as a site of antibody production was great when we analysed results as the true contribution of the total number of antitoxin antibody-forming cells, taking into account the number of cells recovered from each organ. When we analysed the anatomical location of memory B and T cells by adoptive transference, we found that cells from mesenteric lymph nodes and spleen were able to transfer a strong antibody response to naive syngeneic recipients, whereas bone marrow cells transferred a weak antibody response.
authors.affiliation: Laboratory of Immunochemistry, Faculty of Exact and Natural Sciences, University of Buenos Aires, Argentina.
authors.forename: R
authors.initials: R
authors.lastname: Benedetti
authors.forename: P
authors.initials: P
authors.lastname: Lev
authors.forename: E
authors.initials: E
authors.lastname: Massouh
authors.forename: J
authors.initials: J
authors.lastname: Fló
mesh_terms: D000284:Administration, Oral; D000818:Animals; D000992:Antitoxins / Q000096:biosynthesis*; D001402:B-Lymphocytes / Q000276:immunology*; D001853:Bone Marrow / Q000276:immunology*; D002772:Cholera Toxin / Q000276:immunology*; D005260:Female; D018928:Immunity, Mucosal; D007070:Immunoglobulin A / Q000096:biosynthesis; D007074:Immunoglobulin G / Q000096:biosynthesis; D007156:Immunologic Memory; D007421:Intestine, Small / Q000276:immunology; D008198:Lymph Nodes / Q000276:immunology; D010581:Peyer's Patches / Q000276:immunology; D051381:Rats; D013154:Spleen / Q000276:immunology; D013601:T-Lymphocytes / Q000276:immunology; D014611:Vaccination
chemical_list: D000992:Antitoxins; D007070:Immunoglobulin A; D007074:Immunoglobulin G; D002772:Cholera Toxin