Mutual activation of two restriction endonucleases: interaction of EcoP1 and EcoP15.
Type III restriction endonucleases recognize nonsymmetric nucleotide sequences. A necessary condition for DNA cleavage is the presence of two unmethylated recognition sites which are inversely ('head-to-head') oriented in the DNA double strand. A DNA substrate possessing one EcoP1 and one EcoP15 site in the head-to-head configuration could not be cleaved by the individual enzymes, however, it was specifically digested in the simultaneous presence of both enzymes. In agreement with the tracking-collision model for the DNA interaction of type III enzymes cleavage could be abolished by Lac repressor bound between the two sites. We conclude that two different type III enzymes can functionally cooperate in the cleavage of DNA.
Kunz
A
A
Institute of Medical Virology, Charité Medical School, Humboldt University, Berlin, Germany.
Mackeldanz
P
P
Mücke
M
M
Meisel
A
A
Reuter
M
M
Schroeder
C
C
Krüger
D H
DH
D019175:DNA Methylation; D015263:Deoxyribonucleases, Type III Site-Specific / Q000378:metabolism*; D008780:Methyltransferases / Q000378:metabolism*; D013379:Substrate Specificity
D008780:Methyltransferases; C030432:endodeoxyribonuclease EcoP1; C023451:endodeoxyribonuclease EcoP15I; D015263:Deoxyribonucleases, Type III Site-Specific