Venous allografts prepared from stripped long saphenous vein. Is there a need for antibiotic sterilisation?
OBJECTIVE
Can useful lengths of vein be retrieved from varicose vein stripping procedures; is it necessary to sterilise this tissue prior to use as vein allografts?


METHODS
Stripped long saphenous vein was retrieved at operation. Vein samples were cultured using direct plate inoculation and enrichment culture. Further samples were immersed in two low concentration antibiotic solutions and recultured. Smooth muscle viability was assessed after antibiotic immersion and storage by cryopreservation.


RESULTS
High quality vein could be retrieved by vein stripping. Vein segments grew skin commensals on enrichment culture despite negative cultures with standard media plate inoculation (Chi-squared = 53.34 1 d.f. p < 0.001). Low concentration antibiotic solutions sterilised processed vein. Smooth muscle cell viability was reduced by cryopreservation, Mann-Whitney p = 0.008 (control 98% S.E. 0.93 vs. cryopreserved 64% S.E. 6.58), but prior exposure to antibiotics did not compound this effect.


CONCLUSIONS
Useful lengths of vein grafts can be retrieved from varicose vein stripping procedures. Venous segments are frequently contaminated by skin commensals. Enrichment culture is required to detect contamination. Low concentration antibiotics sterilise venous-tissue without affecting smooth muscle cell viability.
Ruddle
A C
AC
Department of Vascular Surgery, Southmead Hospital, Bristol, U.K.
George
S
S
Armitage
W J
WJ
MacGowan
A
A
McCulloch
S
S
Brookes
S T
ST
Mitchell
D C
DC
D000900:Anti-Bacterial Agents / Q000627:therapeutic use*; D016009:Chi-Square Distribution; D015925:Cryopreservation; D003470:Culture Media; D046508:Culture Techniques; D006801:Humans; D015378:Imipenem / Q000627:therapeutic use; D007101:Immersion; D007552:Isotonic Solutions / Q000627:therapeutic use; D009131:Muscle, Smooth, Vascular / Q000187:drug effects / Q000473:pathology; D010406:Penicillins / Q000627:therapeutic use; D012501:Saphenous Vein / Q000473:pathology / Q000601:surgery / Q000637:transplantation*; D012867:Skin / Q000382:microbiology; D013210:Staphylococcus / Q000254:growth & development; D013242:Sterilization / Q000379:methods*; D013307:Streptomycin / Q000627:therapeutic use; D013845:Thienamycins / Q000627:therapeutic use; D014021:Tissue Preservation; D014022:Tissue Survival; D014184:Transplantation, Homologous; D014648:Varicose Veins / Q000601:surgery
D000900:Anti-Bacterial Agents; D003470:Culture Media; C072670:Hanks Balanced Salt Solution; D007552:Isotonic Solutions; D010406:Penicillins; D013845:Thienamycins; D015378:Imipenem; D013307:Streptomycin