Transgenesis by adenovirus-mediated gene transfer into mouse zona-free eggs.
Zona-free mouse eggs at the pronucleus stage were infected with a replication-defective adenovirus vector containing a nuclear-targeted lacZ gene. Exogenous beta-galactosidase activity was detected in almost all eggs at the two-cell stage. Of 27 mice that developed from infected eggs, three carried the integrated exogenous gene mediated by the adenovirus. Two of the three expressed the lacZ gene, and all three mice transmitted the adenovirus-mediated transgene to F1 progeny Southern blot analysis was consistent with single copy integration. This finding should accelerate the development of new strategies for transgenesis and assist studies on the function of cloned genes in vivo.
Tsukui
T
T
Department of Reproductive and Developmental Biology, University of Tokyo, Japan. tsukui@hgc.ims.u-tokyo.ac.jp
Kanegae
Y
Y
Saito
I
I
Toyoda
Y
Y
D000256:Adenoviridae / Q000235:genetics*; D000818:Animals; D018014:Gene Transfer Techniques; D005854:Germ Cells; D051379:Mice; D008822:Mice, Transgenic; D010063:Ovum / Q000378:metabolism*; D019076:Transgenes; D016662:Virus Integration; D015044:Zona Pellucida