[Isolation and cloning of gene for hepatitis delta antigen. The use of recombinant antigen for serodiagnosis of delta infection].
Hepatitis delta virus antigen was isolated from a patient by reverse transcription and polymerase chain reaction. Gel-purified cDNA was cloned in E. coli expression vectors. High expression of the recombinant HDAg in bacteria was observed. The minor and major forms of HDV antigens were simultaneously expressed in bacterial strains carrying SupE mutation. A laboratory method is developed for detecting anti-HD in patients' blood. It is based on the use of minor and major forms of recombinant HDV antigen immobilized on the same membrane filter. The method can be used for creating an original highly specific test system.
Ryzhova
E V
EV
Ivaniushina
V A
VA
Grudinin
M P
MP
Ivanova
O V
OV
Vinogradova
E N
EN
Kiselev
O I
OI
D003001:Cloning, Molecular; D018963:Hepatitis Antigens / Q000235:genetics*; D003699:Hepatitis D / Q000175:diagnosis*; D003698:Hepatitis Delta Virus / Q000276:immunology*; D035923:Hepatitis delta Antigens; D006801:Humans; D011994:Recombinant Proteins / Q000276:immunology; D012698:Serologic Tests
D018963:Hepatitis Antigens; D035923:Hepatitis delta Antigens; D011994:Recombinant Proteins; C442244:hepatitis delta virus large antigen
Vydelenie i klonirovanie gena antigena virusa gepatita del'ta. Ispol'zovanie rekombinantnogo antigena dlia serodiagnostiki del'ta-infektsii.