Capillary isotachophoresis with fiber-optic Raman spectroscopic detection. Performance and application to ribonucleotides.
A fiber-optic Raman probe fitted with a microscope objective was used to obtain on-line normal Raman spectra of adenosine 5'-monophosphate, cytidine 5'-monophosphate, guanosine 5'-monophosphate and uridine 5'-monophosphate separated by capillary isotachophoresis. With multimode optical fiber, the system interrograted a 40-micron length of capillary. Fiber-optic coupling facilitated use of an unmodified spectrograph and conventional capillary mounting systems. Raman spectra were excited with a 2W 532 nm NdYVO4, laser as the excitation source, with collection of 1 spectrum per second. Even at 2.10(-5) M initial concentration, Raman spectra were obtained at a good signal-to-noise ratio.
Walker
P A
PA
University of Michigan, Department of Chemistry, Ann Arbor 48109-1055, USA.
Morris
M D
MD
D004586:Electrophoresis; D005336:Fiber Optic Technology; D007202:Indicators and Reagents; D007293:Inosine Triphosphate / Q000032:analysis; D055100:Optical Fibers; D012265:Ribonucleotides / Q000032:analysis*; D013059:Spectrum Analysis, Raman
D007202:Indicators and Reagents; D012265:Ribonucleotides; D007293:Inosine Triphosphate