title: RNA-binding protein conserved in both microtubule- and microfilament-based RNA localization.
abstract: Vg1 mRNA translocation to the vegetal cortex of Xenopus oocytes requires intact microtubules, and a 3' UTR cis-acting element (termed VLE), which also mediates sequence-specific binding of several proteins. One protein, the 69-kD Vg1 RBP, associates Vg1 RNA to microtubules in vitro. Here we show that Vg1 RBP-binding sites correlate with vegetal localization. Purification and cloning of Vg1 RBP revealed five RNA-binding motifs: four KH and one RRM domains. Surprisingly, Vg1 RBP is highly homologous to the zipcode binding protein implicated in the microfilament-mediated localization of beta actin mRNA in fibroblasts. These data support Vg1 RBP's direct role in vegetal localization and suggest the existence of a general, evolutionarily conserved mechanism for mRNA targeting.
authors.affiliation: Department of Anatomy and Cell Biology, Hebrew University Medical School, Jerusalem 91120, Israel.
authors.forename: L
authors.initials: L
authors.lastname: Havin
authors.forename: A
authors.initials: A
authors.lastname: Git
authors.forename: Z
authors.initials: Z
authors.lastname: Elisha
authors.forename: F
authors.initials: F
authors.lastname: Oberman
authors.forename: K
authors.initials: K
authors.lastname: Yaniv
authors.forename: S P
authors.initials: SP
authors.lastname: Schwartz
authors.forename: N
authors.initials: N
authors.lastname: Standart
authors.forename: J K
authors.initials: JK
authors.lastname: Yisraeli
mesh_terms: D008841:Actin Cytoskeleton / Q000378:metabolism*; D000595:Amino Acid Sequence; D000818:Animals; D001665:Binding Sites / Q000235:genetics; D005260:Female; D006023:Glycoproteins / Q000235:genetics / Q000378:metabolism*; D008870:Microtubules / Q000378:metabolism*; D008969:Molecular Sequence Data; D009865:Oocytes / Q000378:metabolism* / Q000648:ultrastructure*; D011485:Protein Binding; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D016601:RNA-Binding Proteins / Q000235:genetics / Q000378:metabolism*; D016415:Sequence Alignment; D016212:Transforming Growth Factor beta; D014981:Xenopus; D029867:Xenopus Proteins
chemical_list: C513103:GDF1 protein, Xenopus; D006023:Glycoproteins; D012333:RNA, Messenger; D016601:RNA-Binding Proteins; D016212:Transforming Growth Factor beta; D029867:Xenopus Proteins