Age-dependent responses of the mesenteric vasculature to ischemia-reperfusion.
The age-dependent responses of the mesenteric vasculature to ischemia-reperfusion (I/R) were compared in 2-mo-old and 2-yr-old rats. Measurements were made of leukocyte adherence, albumin leakage, and oxidative stress in postcapillary venules. In young rats I/R induced an increase in leukocyte adherence and albumin leakage, but in aged rats I/R induced an increase in albumin leakage without an increase in leukocyte adherence. Furthermore, I/R-induced oxidative stress was higher in the aged rats than in the young rats. To investigate whether the age-associated oxidative stress is related to a decrease in the role of nitric oxide, NG-nitro-L-arginine methyl ester (L-NAME) was superfused onto the mesentery of young and aged rats. L-NAME induced an increase in postcapillary protein leakage only in young rats; however, arteriolar constriction induced by L-NAME occurred in both age groups. These results suggest that different mechanisms contribute to the inflammatory responses and microvascular dysfunction elicited by I/R in young and aged rats.
Harris
N R
NR
Department of Molecular and Cellular Physiology, Louisiana State University Medical Center, Shreveport 71103, USA.
Langlois
K W
KW
D000375:Aging / Q000502:physiology*; D000818:Animals; D002448:Cell Adhesion; D007962:Leukocytes / Q000473:pathology; D008297:Male; D008643:Mesentery / Q000098:blood supply* / Q000473:pathology / Q000503:physiopathology*; D018384:Oxidative Stress; D051381:Rats; D011916:Rats, Inbred F344; D015427:Reperfusion Injury / Q000473:pathology / Q000503:physiopathology*; D012709:Serum Albumin / Q000502:physiology; D013152:Splanchnic Circulation / Q000502:physiology*
D012709:Serum Albumin