title: [An attempt to detect Trypanosoma cruzi in the peripheral blood of patients with Chagas' disease, in chronic phase, using quantitative buffy coat (QBC)].
abstract: Taking for granted the sensitivity of the Quantitative Buffy Coat (QBC) system, as documented in a murine experimental model, we assayed to detect Trypanosoma cruzi in the peripheral blood of 100 patients with Chagas disease in its chronic phase. By means of the method, no positivity occurred, evently as a consequence of small parasitemias, undetectable by this technique as assessed by the cases in consideration.
authors.affiliation: Laboratório de Investigação Médica-Parasitologia do Hospital da Clínicas, da Faculdade de Medicina, Universidade de São Paulo, Brasil.
authors.forename: V
authors.initials: V
authors.lastname: Amato Neto
authors.forename: M H
authors.initials: MH
authors.lastname: Lopes
authors.forename: C R
authors.initials: CR
authors.lastname: De Marchi
authors.forename: M de F
authors.initials: Mde F
authors.lastname: Silva
mesh_terms: D000165:Acridine Orange; D000328:Adult; D000368:Aged; D000818:Animals; D014355:Chagas Disease / Q000097:blood / Q000469:parasitology*; D002908:Chronic Disease; D005260:Female; D005456:Fluorescent Dyes; D006801:Humans; D008297:Male; D008875:Middle Aged; D014349:Trypanosoma cruzi / Q000276:immunology / Q000302:isolation & purification*
chemical_list: D005456:Fluorescent Dyes; D000165:Acridine Orange
vernacular_title: Tentativa de evidenciar o Trypanosoma cruzi no sangue periférico de pacientes com doença de Chagas, em fase crônica, por meio do Quantitative Buffy coat (QBC).