title: Genomic organization of the 3'-region of the human MUC5AC mucin gene: additional evidence for a common ancestral gene for the 11p15.5 mucin gene family.
abstract: The human mucin gene MUC5AC is mapped clustered with MUC2, MUC5B and MUC6 on chromosome 11p15.5. We report here the isolation and characterization of a genomic cosmid clone, designated ELO9, spanning the 3'-region of MUC5AC and the 5'-region of MUC5B, allowing us to conclude that MUC5AC and MUC5B have the same transcriptional orientation. We determined the genomic organization and the entire sequence of the 3'-region of MUC5AC. The comparative molecular analysis of MUC5AC and MUC5B points to a remarkable similarity in the size and the distribution of exons, and in the type of splice sites, supporting the notion that MUC5AC and MUC5B have evolved from a single common ancestral gene. The derivation of the four genes of the 11p15.5 mucin gene family from a single ancestral gene is discussed.
authors.affiliation: INSERM Unité 377, Place de Verdun, 59045 Lille Cedex, France.
authors.forename: M P
authors.initials: MP
authors.lastname: Buisine
authors.forename: J L
authors.initials: JL
authors.lastname: Desseyn
authors.forename: N
authors.initials: N
authors.lastname: Porchet
authors.forename: P
authors.initials: P
authors.lastname: Degand
authors.forename: A
authors.initials: A
authors.lastname: Laine
authors.forename: J P
authors.initials: JP
authors.lastname: Aubert
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D002880:Chromosomes, Human, Pair 11 / Q000235:genetics*; D003001:Cloning, Molecular; D004247:DNA / Q000235:genetics / Q000378:metabolism; D018076:DNA, Complementary / Q000235:genetics; D015698:Genomic Library; D006801:Humans; D008969:Molecular Sequence Data; D055271:Mucin 5AC; D055262:Mucin-2; D055257:Mucin-5B; D055272:Mucin-6; D009077:Mucins / Q000235:genetics*; D016133:Polymerase Chain Reaction; D014132:Trachea / Q000378:metabolism
chemical_list: D018076:DNA, Complementary; C524625:MUC2 protein, human; C524678:MUC5AC protein, human; C524578:MUC5B protein, human; C524734:MUC6 protein, human; D055271:Mucin 5AC; D055262:Mucin-2; D055257:Mucin-5B; D055272:Mucin-6; D009077:Mucins; D004247:DNA