title: Modeling the tertiary interactions in the eukaryotic selenocysteine tRNA.
abstract: A novel three-dimensional model of tertiary interactions in the core region of the eukaryotic selenocysteine tRNA is proposed based on the analysis of available nucleotide sequences. The model features the 7/5 tRNA(Sec) secondary structure characterized by seven and five base pairs in the acceptor and T-stems, respectively, and four nucleotides in the connector region between the acceptor and D-stems. The model suggests a unique system of tertiary interactions in the area between the major groove of the D-stem and the first base pair of the extra arm that provides a rigid orientation of the extra arm and contributes to the overall stability of the molecule. The model is consistent with available experimental data on serylation, selenylation, and phosphorylation of different tRNA(Sec) mutants. The important similarity between the proposed model and the structure of the tRNA(Ser) is shown. Based on this similarity, the ability of some tRNA(Ser) mutants to be serylated, selenylated, and phosphorylated was evaluated and found to be in a good agreement with experimental data.
authors.affiliation: Département de Biochimie, Université de Montréal, Québec, Canada.
authors.forename: A
authors.initials: A
authors.lastname: Ioudovitch
authors.forename: S V
authors.initials: SV
authors.lastname: Steinberg
mesh_terms: D000818:Animals; D001483:Base Sequence; D003196:Computer Graphics; D003198:Computer Simulation; D005057:Eukaryotic Cells; D006801:Humans; D008958:Models, Molecular; D008969:Molecular Sequence Data; D009154:Mutation; D009690:Nucleic Acid Conformation; D012345:RNA, Transfer, Amino Acid-Specific / Q000737:chemistry* / Q000235:genetics; D012362:RNA, Transfer, Ser / Q000737:chemistry / Q000235:genetics; D012689:Sequence Homology, Nucleic Acid
chemical_list: D012345:RNA, Transfer, Amino Acid-Specific; D012362:RNA, Transfer, Ser; C036928:tRNA, selenocysteine-