title: Modifications in protein binding to upstream sequences of the sea urchin cytoplasmic actin gene CyIIa in comparison to its linked neighbors, CyI and CyIIb.
abstract: The sequences corresponding to regions upstream of the ATG and transcription start site of the CyIIa cytoplasmic actin gene of the sea urchin Strongylocentrotus purpuratus were determined and compared to the genomically linked CyI and CyIIb actin genes. Sites of protein-DNA interaction in the CyIIa upstream sequences were identified by DNase I footprinting. The similarity between CyIIa and CyI (and CyIIb) upstream sequences was limited and included a consensus octamer sequence, serum response element (SRE) and some short sequences within the proximal promoter region. The octamer sequence was found to bind protein. A single DNase I hypersensitive site was detected within the SRE and to two flanking nucleotides, but otherwise, the SRE did not appear to be protected. This is in contrast to strong protein binding to the CyIIb SRE. A region in the CyIIa gene with limited identity to the functionally significant protein binding site D in CyI also did not bind protein. Four additional sites of protein-DNA interaction were identified in CyIIa upstream sequences. One of these is similar to a protein binding site previously located in the CyI upstream sequences, whereas the others appear to be unique. These data indicate that the CyIIa upstream sequences differ extensively from those of CyI. The pattern of CyIIa expression is likely a consequence of these alternations in DNA sequence and protein-DNA interactions.
authors.affiliation: Department of Biology, University of North Carolina at Greensboro, Greensboro, NC 27412, USA. katulak@goodall.uncg.edu
authors.forename: K S
authors.initials: KS
authors.lastname: Katula
authors.forename: R L
authors.initials: RL
authors.lastname: Dukes
authors.forename: H
authors.initials: H
authors.lastname: Paul
authors.forename: R R
authors.initials: RR
authors.lastname: Franks
mesh_terms: D000199:Actins / Q000235:genetics*; D000818:Animals; D001483:Base Sequence; D016384:Consensus Sequence; D003593:Cytoplasm / Q000737:chemistry; D018983:DNA Footprinting; D005786:Gene Expression Regulation; D005796:Genes; D008969:Molecular Sequence Data; D011401:Promoter Regions, Genetic; D011485:Protein Binding; D012045:Regulatory Sequences, Nucleic Acid; D012617:Sea Urchins / Q000235:genetics*; D012689:Sequence Homology, Nucleic Acid
chemical_list: D000199:Actins