Evaluation of hair root analysis for acute phencyclidine poisoning and behavior of phencyclidine metabolites in rat hair root.
We evaluated the usefulness of hair root analysis to diagnose acute phencyclidine (PCP) poisoning. Male rats were i.p. administered acute poisonous doses (80, 100 and 120 mg/kg) of PCP hydrochloride and the hair roots were plucked out with hair nippers at certain times after administration. The hair root samples were extracted with methanol/HCl. After evaporation of the solvent, the residue was derivatized with N,O-bis(trimethylsilyl) acetamide and analyzed with GC/MS. PCP was detected at high concentrations (up to 181.7 ng/mg) from all samples. The peak concentrations at every dose were observed at 6 h. The concentrations of PCP in the rat hair roots increased dose-dependently in the range of the doses. 1-(1-Phenylcyclohexyl)-4-hydroxypiperidine (PCHP) and trans-1-phenyl-1(4'-hydroxypiperidino)-4-cyclohexanol (t-PCPdiol) were also detected from 5 and 15 min to 48 h after administration, respectively. It is concluded that hair root is a useful specimen for the diagnosis of acute PCP poisoning because PCP, PCHP and t-PCPdiol are detected very soon after administration and a large amount of them is retained in hair root for a long time. PCHP was found from the early stage in hair roots and its concentration was higher than that of t-PCPdiol for 6 h. However, the concentration of t-PCPdiol became higher than that of PCHP after 6 h. These phenomena could be explained by the time lag of production of the primary (PCHP) and the secondary metabolite (PCPdiol).
Sakamoto
T
T
Laboratory of Drug Analysis, Showa College of Pharmaceutical Sciences, Tokyo, Japan.
Endo
M
M
Nagasaki
A
A
Nakamura
A
A
Watanabe
S
S
Tanaka
A
A
Nakahara
Y
Y
D000208:Acute Disease; D000818:Animals; D001711:Biotransformation; D002138:Calibration; D008401:Gas Chromatography-Mass Spectrometry; D006197:Hair / Q000737:chemistry*; D006213:Hallucinogens / Q000032:analysis* / Q000493:pharmacokinetics / Q000506:poisoning; D008297:Male; D010622:Phencyclidine / Q000031:analogs & derivatives / Q000032:analysis* / Q000493:pharmacokinetics / Q000506:poisoning; D010880:Piperidines / Q000032:analysis; D051381:Rats
C109379:1-(1-phenyl-4-hydroxycyclohexyl)-4'-hydroxypiperidine; D006213:Hallucinogens; D010880:Piperidines; C031108:1-(1-phenylcyclohexyl)-4-hydroxypiperidine; D010622:Phencyclidine