title: Heterodimer SRP9/14 is an integral part of the neural BC200 RNP in primate brain.
abstract: BC200 RNA is a brain-specific, small non-messenger RNA with a somatodendritic localization in primate neurons and a constituent of a ribonucleoprotein (RNP) complex. The primary and secondary structure of the 5' domain of BC200 RNA resembles that of the Alu domain of 7SL RNA, which is an integral part of the signal recognition particle (SRP). This would predict that similar proteins bind to this defined domain of both RNA species in vitro and in vivo. The data presented in this paper reveal that a protein that binds BC200 RNA in vivo is immunoreactive with antibodies against SRP9. This further supports the notion that the 5' domain of the BC200 RNA can fold into structures similar to the SRP Alu domain and, as a result, bind identical or similar proteins in vivo. The SRP9 protein binds only as dimer with SRP14 protein to the Alu domain of 7SL RNA to form a subdomain that, in SRP, is functional in translation arrest. Therefore, our data also indicate that the neuronal BC200 RNP is a candidate for regulating decentralized protein biosynthesis in dendrites, possibly with a mechanism that resembles translation arrest of the SRP.
authors.affiliation: Institute for Experimental Pathology, Center for Molecular Biology of Inflammation, University of Münster, Germany.
authors.forename: J
authors.initials: J
authors.lastname: Kremerskothen
authors.forename: D
authors.initials: D
authors.lastname: Zopf
authors.forename: P
authors.initials: P
authors.lastname: Walter
authors.forename: J G
authors.initials: JG
authors.lastname: Cheng
authors.forename: M
authors.initials: M
authors.lastname: Nettermann
authors.forename: U
authors.initials: U
authors.lastname: Niewerth
authors.forename: R J
authors.initials: RJ
authors.lastname: Maraia
authors.forename: J
authors.initials: J
authors.lastname: Brosius
mesh_terms: D000818:Animals; D002457:Cell Extracts / Q000737:chemistry; D003712:Dendrites / Q000737:chemistry* / Q000502:physiology; D004285:Dogs; D006801:Humans; D009690:Nucleic Acid Conformation; D011323:Primates; D011485:Protein Binding / Q000502:physiology; D014176:Protein Biosynthesis / Q000502:physiology*; D012342:RNA, Small Nuclear / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D016601:RNA-Binding Proteins / Q000235:genetics* / Q000276:immunology / Q000494:pharmacology; D012261:Ribonucleoproteins / Q000235:genetics* / Q000378:metabolism; D018271:Signal Recognition Particle / Q000235:genetics* / Q000276:immunology / Q000494:pharmacology
chemical_list: D002457:Cell Extracts; D012342:RNA, Small Nuclear; D016601:RNA-Binding Proteins; D012261:Ribonucleoproteins; C486350:SRP9 protein, human; D018271:Signal Recognition Particle