Automatic cell electrorotation measurements: studies of the biological effects of low-frequency magnetic fields and of heat shock.
A computer-aided automatic imaging technique has been developed for measuring the electrorotation spectra of up to 256 particles at the same time. This offers advantages over the conventional manual method, especially when rapidly acquired statistical data are necessary in investigations of the response of cells or test beads to chemical exposure, for example. We have applied this technique to investigate the biological effects of heat shock and low-frequency EM fields reported by others for yeast cells. Although heat shock effects were observed, no changes of the electrorotational behaviour could be detected after exposing the cells to 50 Hz, 8 and 80 microT fields. Although this does not rule out the possibility that the cells were influenced by the magnetic fields, it does limit the number of possible physicochemical changes that might have occurred to their cell walls and membranes.
Zhou
X F
XF
Institute of Molecular & Biomolecular Electronics, University of Wales, Bangor, Gwynedd, UK.
Burt
J P
JP
Pethig
R
R
D001331:Automation; D003198:Computer Simulation; D004574:Electromagnetic Fields; D006358:Hot Temperature; D008962:Models, Theoretical; D012399:Rotation; D012441:Saccharomyces cerevisiae / Q000166:cytology / Q000254:growth & development* / Q000528:radiation effects