Differential gene expression of a human alpha2,3-sialyltransferase in leukaemic cell lines and leucocytes.
The gene expression of the human Gal beta1,4(3)GlcNAc/Gal beta1,3GalNAc alpha-2,3-sialyltransferase was investigated in the leukaemic cell lines HL60, K-562, MOLT-4, THP-1 and in blood leucocytes. Five different transcripts were identified. In HL60 and THP-1 cells the expression levels of two of these changed during differentiation. Two potential AP1 binding sites were detected in the promoter regions of the gene. THP-1 cells contain proteins binding with higher affinities to these sequences in the sialyltransferase gene than to the AP1 consensus sequence, whereas nuclear extracts from HL60 cells have the opposite affinity.
Gassen
U
U
Biochemisches Institut, Christian-Albrechts-Universität Kiel, Germany.
Kelm
S
S
Schauer
R
R
D015870:Gene Expression; D018922:HL-60 Cells / Q000201:enzymology; D006801:Humans; D007962:Leukocytes / Q000201:enzymology*; D009363:Neoplasm Proteins / Q000235:genetics*; D009687:Nuclear Proteins / Q000378:metabolism; D012799:Sialyltransferases / Q000235:genetics* / Q000378:metabolism; D018808:Transcription Factor AP-1 / Q000235:genetics / Q000378:metabolism; D014158:Transcription, Genetic; D014407:Tumor Cells, Cultured
D009363:Neoplasm Proteins; D009687:Nuclear Proteins; D018808:Transcription Factor AP-1; D012799:Sialyltransferases