Modulation of eosinophil chemotactic activities to leukotriene B4 by n-3 polyunsaturated fatty acids.
Eosinophil accumulation induced by leukotriene B4 appears to be involved in the pathogenesis of allergic diseases. We evaluated the effects of docosahexaenoic acid (DHA) and eicosapentaenoic acid (EPA) on chemotaxis to leukotriene B4 in guinea pig peritoneal eosinophils. Guinea pigs that were sensitized to polymyxin B were administered an intraperitoneal injection of polymyxin B (1 mg/animal) alone or combined with DHA (15 or 50 mg/kg, i.p.), EPA (50 or 100 mg/kg, i.p.), or with linoleic acid (LA) (100 mg/kg, i.p.). Forty hours later, eosinophils were obtained from the intraperitoneal lavage fluid and purified. The chemotactic and chemokinetic responses of eosinophils to leukotriene B4 were measured using a 96-well microchemotaxis chamber. DHA significantly decreased the chemotactic and chemokinetic responses of eosinophils in a dose-dependent fashion. A higher dose of EPA also significantly inhibited both of those responses, whereas LA had no effect. Our results suggested a possible mechanism for the improvement of allergic diseases by dietary supplementation with n-3 PUFA.
Kikuchi
S
S
Department of Paediatrics, Nagoya University School of Medicine, Japan.
Sakamoto
T
T
Ishikawa
C
C
Yazawa
K
K
Torii
S
S
D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology; D002465:Cell Movement / Q000187:drug effects; D018925:Chemokines / Q000037:antagonists & inhibitors / Q000494:pharmacology; D002631:Chemotactic Factors, Eosinophil / Q000502:physiology*; D002634:Chemotaxis, Leukocyte / Q000187:drug effects*; D004281:Docosahexaenoic Acids / Q000008:administration & dosage / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D015118:Eicosapentaenoic Acid / Q000008:administration & dosage / Q000494:pharmacology; D004804:Eosinophils / Q000166:cytology*; D005227:Fatty Acids / Q000737:chemistry; D015525:Fatty Acids, Omega-3 / Q000494:pharmacology*; D006168:Guinea Pigs; D007975:Leukotriene B4 / Q000276:immunology*; D019787:Linoleic Acid / Q000494:pharmacology; D008297:Male; D010743:Phospholipids / Q000737:chemistry; D011112:Polymyxin B / Q000494:pharmacology; D015203:Reproducibility of Results
D000900:Anti-Bacterial Agents; D018925:Chemokines; D002631:Chemotactic Factors, Eosinophil; D005227:Fatty Acids; D015525:Fatty Acids, Omega-3; D010743:Phospholipids; D007975:Leukotriene B4; D004281:Docosahexaenoic Acids; D019787:Linoleic Acid; D015118:Eicosapentaenoic Acid; D011112:Polymyxin B