Retroviral-mediated expression of FIV envelope/Rev induces CD8+ CTL responses in mice.
Recombinant retroviral vectors that express the Env and Rev proteins of feline immunodeficiency virus (FIV) were prepared and analyzed in a mouse model system for their ability to induce antigen-specific CD8+ CTL (cytotoxic T lymphocyte) responses. The ultimate goal of these studies is to develop effective immunogens for CTL induction in the cat. Recombinant Env/Rev retroviral vectors were used to transduce mouse fibroblasts and these cells were then inoculated intraperitoneally into syngeneic BALB/c mice. FIV Env-specific CTL responses were obtained. The potent ability of recombinant retroviral vector-transduced cells to elicit cell-mediated immunity in the mouse model offers the possibility that such delivery systems may serve as therapeutic and/or prophylactic treatments against FIV infection in the cat.
von Schwedler
U
U
Chiron Technologies Center for Gene Therapy, San Diego, Calif. 92121, USA.
Townsend
K
K
Chada
S
S
Jolly
D J
DJ
Elder
J
J
Chang
S M
SM
Lee
W T
WT
D000818:Animals; D002415:Cats; D015870:Gene Expression; D015694:Gene Products, rev / Q000235:genetics / Q000276:immunology*; D005822:Genetic Vectors; D016089:Immunodeficiency Virus, Feline / Q000235:genetics / Q000276:immunology*; D051379:Mice; D008807:Mice, Inbred BALB C; D011994:Recombinant Proteins / Q000235:genetics / Q000276:immunology; D012190:Retroviridae / Q000235:genetics*; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology*; D014759:Viral Envelope Proteins / Q000235:genetics / Q000276:immunology*
D015694:Gene Products, rev; D011994:Recombinant Proteins; D014759:Viral Envelope Proteins