An improved serum free system for cloning human "pure" erythroid colonies. The role of different growth factors and cytokines on BFU-E formation by the bone marrow and cord blood CD34+ cells.
We have developed an efficient serum free culture model for cloning human erythroid progenitors. Accordingly, human bone marrow or cord blood CD34+ cells if plated in our serum free medium and stimulated with a mixture of EpO + KL, grow erythroid colonies exclusively. Cells isolated from these cultures express glycophorin-A (GPA-A), are CD33-, IIb/IIIa-, and finally all become hemoglobinized. By employing this system we also found out that cord blood CD34+ mononuclear cells (MNC) contain more BFU-E than adult marrow CD34+ MNC, moreover, the erythroid colonies formed by cord blood progenitors are significantly larger then the ones formed by the marrow cells. We have also compared the influence of different cytokines and growth factors, which were reported in the literature to costimulate BFU-E growth on cloning efficiency of human BFU-E cultured in our serum free medium. We found that from 20 different growth factors and cytokines tested, EpO dependent bone marrow BFU-E growth is costimulated only by KL, and to lesser degree also by IL-3, GM-CSF, TpO and IL-9. In contrast to marrow cells we observed that cord blood BFU-E in addition to KL, IL-3, GM-CSF, TpO, LIF and IL-9 were also costimulated by NGF-beta, FGF-1, FGF-2 and STK-IL. We found simultaneously that TPO which possess only negligible costimulatory effect on erythroid colony formation by bone marrow CD34+ cells, significantly costimulated the formation of erythroid colonies grown by cord blood CD34+ cells. Therefore, the cord blood CD34+ cells are largely committed to erythroid differentiation, and, moreover, they respond to a wider spectrum of the growth factors than their bone marrow counterparts.
Ratajczak
J
J
Department of Pathology, University of Pennsylvania School of Medicine, Philadelphia, USA.
Marlicz
W
W
Machaliński
B
B
Pertusini
E
E
Czajka
R
R
Ratajczak
M Z
MZ
D018952:Antigens, CD34 / Q000276:immunology; D001853:Bone Marrow / Q000378:metabolism; D002999:Clone Cells / Q000166:cytology*; D046508:Culture Techniques / Q000379:methods*; D016207:Cytokines / Q000502:physiology*; D015672:Erythroid Precursor Cells / Q000166:cytology* / Q000502:physiology*; D004921:Erythropoietin / Q000494:pharmacology; D005312:Fetal Blood / Q000378:metabolism; D005434:Flow Cytometry; D006021:Glycophorins / Q000378:metabolism; D006133:Growth Substances / Q000502:physiology*; D006454:Hemoglobins / Q000378:metabolism; D006801:Humans; D019089:Stem Cell Factor / Q000494:pharmacology
D018952:Antigens, CD34; D016207:Cytokines; D006021:Glycophorins; D006133:Growth Substances; D006454:Hemoglobins; D019089:Stem Cell Factor; D004921:Erythropoietin