[Effect of verapamil on the activity of Ca2+-, Mg2+-dependent endonucleases in hypothalamic cell nuclei in long-term hypovolemic shock].
Experiments on anesthetized non-inbred 8-17 kg b.w.dogs have demonstrated that cerebral ischemia consequent to 4-hour hypovolemic shock activates Ca(2+)-, Mg(2+)-dependent endonucleases in cell nuclei of the hypothalamus. Intravenous injection of calcium channels blocker verapamil (Orion) in a dose 0.1 mg/kg 30 min before blood loss prevents activation of the above endonucleases and makes it possible to avoid internucleosome fragmentation of genome DNA.
Kozhura
V L
VL
Nosova
N V
NV
Novoderzhkina
I S
IS
D000818:Animals; D002118:Calcium / Q000494:pharmacology*; D002121:Calcium Channel Blockers / Q000627:therapeutic use*; D002467:Cell Nucleus / Q000187:drug effects* / Q000201:enzymology; D004285:Dogs; D004720:Endonucleases / Q000187:drug effects*; D005260:Female; D007031:Hypothalamus / Q000187:drug effects* / Q000201:enzymology / Q000648:ultrastructure; D008274:Magnesium / Q000494:pharmacology*; D008297:Male; D012769:Shock / Q000188:drug therapy* / Q000201:enzymology / Q000473:pathology; D014700:Verapamil / Q000627:therapeutic use*
D002121:Calcium Channel Blockers; D014700:Verapamil; D004720:Endonucleases; D008274:Magnesium; D002118:Calcium
Vliianie verapamila na aktivnost' Ca2+-, Mg+-zavisimykh éndonukleas v kletochnykh iadrakh gipotalamusa pri dlitel'nom gipovolemicheskom shoke.