Contamination of mouse-adapted influenza virus with Sendai virus.
In our laboratory animal facility, Sendai virus (HVJ) contamination occurred in a negative flow rack used for experimental infection with 4 strains of mouse-adapted influenza virus (Inf.V). Anti-HVJ antibody (Ab) was detected in 35/42 mice in the rack. To specify the strain of Inf.V contaminated with HVJ, experimental infection was performed by using A, B and D strains of Inf.V in each vinyl isolator. Anti-HVJ Ab was detected in all mice infected with A strain at day 28 post-infection. As a result of experimental infection with A strain of Inf.V which was treated with anti-HVJ mouse serum, the virus suspension was determined not to contain HVJ and allowed for experimental use in our facility, Since then, HVJ contamination has not occurred in our facility.
Takimoto
K
K
Division of Experimental Animal Research, National Institute of Infectious Diseases, Tokyo, Japan.
Nakayama
K
K
Yabe
M
M
Ami
Y
Y
Yamada
Y K
YK
Tamura
S
S
Suzuki
Y
Y
Asano
T
T
Saito
M
M
D000818:Animals; D000914:Antibodies, Viral / Q000097:blood; D004797:Enzyme-Linked Immunosorbent Assay; D051379:Mice; D008807:Mice, Inbred BALB C; D008809:Mice, Inbred C3H; D009975:Orthomyxoviridae / Q000276:immunology; D009976:Orthomyxoviridae Infections / Q000276:immunology / Q000821:virology*; D018108:Respirovirus / Q000276:immunology; D010253:Respirovirus Infections / Q000276:immunology / Q000821:virology*; D014765:Viral Vaccines / Q000276:immunology
D000914:Antibodies, Viral; D014765:Viral Vaccines