title: Antigenic and genomic diversity of human rotavirus VP4 in two consecutive epidemic seasons in Mexico.
abstract: In the present investigation we characterized the antigenic diversity of the VP4 and VP7 proteins in 309 and 261 human rotavirus strains isolated during two consecutive epidemic seasons, respectively, in three different regions of Mexico. G3 was found to be the prevalent VP7 serotype during the first year, being superseded by serotype G1 strains during the second season. To antigenically characterize the VP4 protein of the strains isolated, we used five neutralizing monoclonal antibodies (MAbs) which showed specificity for VP4 serotypes P1A, P1B, and P2 in earlier studies. Eight different patterns of reactivity with these MAbs were found, and the prevalence of three of these patterns varied from one season to the next. The P genotype of a subset of 52 samples was determined by PCR. Among the strains characterized as genotype P[4] and P[8] there were three and five different VP4 MAb reactivity patterns, respectively, indicating that the diversity of neutralization epitopes in VP4 is greater than that previously appreciated by the genomic typing methods.
authors.affiliation: Instituto de Biotecnología, Universidad Nacional Autónoma de México, Cuernavaca, Morelos.
authors.forename: L
authors.initials: L
authors.lastname: Padilla-Noriega
authors.forename: M
authors.initials: M
authors.lastname: Méndez-Toss
authors.forename: G
authors.initials: G
authors.lastname: Menchaca
authors.forename: J F
authors.initials: JF
authors.lastname: Contreras
authors.forename: P
authors.initials: P
authors.lastname: Romero-Guido
authors.forename: F I
authors.initials: FI
authors.lastname: Puerto
authors.forename: H
authors.initials: H
authors.lastname: Guiscafré
authors.forename: F
authors.initials: F
authors.lastname: Mota
authors.forename: I
authors.initials: I
authors.lastname: Herrera
authors.forename: R
authors.initials: R
authors.lastname: Cedillo
authors.forename: O
authors.initials: O
authors.lastname: Muñoz
authors.forename: J
authors.initials: J
authors.lastname: Calva
authors.forename: M L
authors.initials: ML
authors.lastname: Guerrero
authors.forename: B S
authors.initials: BS
authors.lastname: Coulson
authors.forename: H B
authors.initials: HB
authors.lastname: Greenberg
authors.forename: S
authors.initials: S
authors.lastname: López
authors.forename: C F
authors.initials: CF
authors.lastname: Arias
mesh_terms: D000911:Antibodies, Monoclonal; D000940:Antigenic Variation; D000956:Antigens, Viral; D002213:Capsid / Q000235:genetics* / Q000276:immunology*; D036022:Capsid Proteins; D003967:Diarrhea / Q000821:virology; D004196:Disease Outbreaks; D004797:Enzyme-Linked Immunosorbent Assay; D005243:Feces / Q000821:virology; D005260:Female; D014644:Genetic Variation; D005838:Genotype; D006801:Humans; D007223:Infant; D007231:Infant, Newborn; D008297:Male; D008800:Mexico / Q000453:epidemiology; D016133:Polymerase Chain Reaction; D012401:Rotavirus / Q000145:classification / Q000235:genetics* / Q000276:immunology* / Q000302:isolation & purification; D012400:Rotavirus Infections / Q000453:epidemiology / Q000821:virology*; D012703:Serotyping
chemical_list: D000911:Antibodies, Monoclonal; D000956:Antigens, Viral; D036022:Capsid Proteins; C060339:VP4 protein, Rotavirus