Metabolic evolution in alpha-amylases from Drosophila virilis and D. repleta, two species with different ecological niches.
alpha-Amylases from Drosophila virilis and D. repleta were partially purified by ion exchange chromatography. The two amylases share common characteristics for pH and cations effects, although with slight differences. D. virilis has optimal activity at pH 6.6 and D. repleta at pH 7.2. Calcium, sodium, and potassium cations activate amylolytic activity in both species but Ba2+ has an activation effect in D. repleta only. In contrast, there are major differences in thermal offbility and kinetics among amylases of the two species. D. virilis amylase is much more stable at high temperature and the optimal temperatures are very different between the two species, respectively, 45 degrees C and 30 degrees C for D. virilis and D. repleta. alpha-Amylase activity using different substrates is greater on starch than on glycogen in both species and still higher on amylose for D. virilis, the nonfungus feeder species. alpha-Amylase of D. repleta, the mycophagous species, has a better affinity to amylopectin and glycogen. Such differences in substrate specificity suggest adaptation to different resources in these species living in different habitats. Metabolic evolution seems to have occurred through a "tradeoff" between kinetic effectiveness and the nature of substrate, with a higher Vmax on amylose for D. virilis and a lower K(m) on glycogen for D. repleta.
Prigent
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S
Populations, Génétique et Evolution, CNRS, Gif-sur-Yvette, France.
Matoub
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M
Rouland
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Cariou
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ML
D000222:Adaptation, Physiological; D000818:Animals; D002412:Cations / Q000494:pharmacology; D004330:Drosophila / Q000235:genetics* / Q000378:metabolism; D017753:Ecosystem; D019143:Evolution, Molecular; D006863:Hydrogen-Ion Concentration; D007527:Isoenzymes / Q000235:genetics* / Q000378:metabolism; D007700:Kinetics; D013045:Species Specificity; D013379:Substrate Specificity; D013696:Temperature; D000516:alpha-Amylases / Q000235:genetics* / Q000378:metabolism
D002412:Cations; D007527:Isoenzymes; D000516:alpha-Amylases