Expression of ORF A1 of infectious bursal disease virus results in the formation of virus-like particles.
A recombinant vaccinia virus inducibly expressing ORF A1 of infectious bursal disease virus (IBDV) has been constructed and characterized. Cells infected with this recombinant virus express the IBDV polyprotein, which is proteolytically processed to give mature VP2, VP3, and VP4 polypeptides. An electron microscopy study revealed that the cytoplasm of cells infected with the recombinant virus contains abundant IBDV-like particles (VLP). These VLP form close-packed paracrystalline arrays that are specifically recognized by anti-IBDV antibodies. The size and morphology of purified VLP were found to be akin to those of authentic IBDV particles.
Fernández-Arias
A
A
Departamento de Biología Molecular y Celular, Centro Nacional de Biotecnología, Cantoblanco, Madrid, Spain.
Risco
C
C
Martínez
S
S
Albar
J P
JP
Rodríguez
J F
JF
D000595:Amino Acid Sequence; D000818:Animals; D002460:Cell Line; D002522:Chlorocebus aethiops; D015870:Gene Expression; D005822:Genetic Vectors; D007243:Infectious bursal disease virus / Q000235:genetics / Q000302:isolation & purification / Q000502:physiology* / Q000648:ultrastructure; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D011498:Protein Precursors / Q000235:genetics; D011817:Rabbits; D011995:Recombination, Genetic; D014764:Viral Proteins / Q000235:genetics* / Q000502:physiology; D015678:Viral Structural Proteins / Q000235:genetics / Q000502:physiology; D019065:Virus Assembly / Q000502:physiology*
D011498:Protein Precursors; C082513:VP2 protein, infectious bursal disease virus; D014764:Viral Proteins; D015678:Viral Structural Proteins