Improvement of transduction efficiency of recombinant adeno-associated virus vector by entrapment in multilamellar liposomes.
Recombinant adeno-associated virus (AAV) has attracted considerable interest as a potential vector for human gene therapy, but its transduction efficiency is quite low. The present study demonstrated AAV vector-associated liposomes to be more effective for in vitro gene transfer to human glioma cells than are liposomes containing plasmid DNA. Using vector-associated liposomes increased the transduction efficiency more than 10-fold compared to liposomes containing plasmid DNA and more than 6-fold compared to AAV alone. From these results, AAV vector-associated liposomes appear to be a good candidate for in vivo gene delivery to human gliomas.
Mizuno
M
M
Department of Neurosurgery, Nagoya University School of Medicine.
Yoshida
J
J
D004279:DNA, Viral / Q000235:genetics; D000229:Dependovirus / Q000201:enzymology / Q000235:genetics*; D005822:Genetic Vectors / Q000378:metabolism*; D005910:Glioma / Q000201:enzymology / Q000235:genetics*; D006801:Humans; D007763:Lac Operon / Q000235:genetics; D008081:Liposomes / Q000378:metabolism; D013997:Time Factors; D014162:Transfection / Q000379:methods*; D014407:Tumor Cells, Cultured; D001616:beta-Galactosidase / Q000378:metabolism
D004279:DNA, Viral; D008081:Liposomes; D001616:beta-Galactosidase