title: Effect on colon cancer cells of human interferon-beta gene entrapped in cationic multilamellar liposomes.
abstract: When cultured cells of human colon cancer cell line SW480 were transfected with human interferon-beta (hIFN-beta) gene by means of cationic multilamellar liposomes, the endogenously produced hIFN-beta exhibited a remarkable anti-proliferative effect on the cells, which was more effective than that of exogenously added hIFN-beta. This effect lasted for several days, and was blocked completely by the addition of sufficient amounts of anti-hIFN-beta antibody. From experiments using a transwell plate and an infusion pump, we found that endogenously produced hIFN-beta acted effectively on the cells around the transfectants and that the growth-inhibitory effect was totally retained upon continuous dilution of the medium. These data indicate that hIFN-beta expressed endogenously by transfer of its gene acted on these cancer cells mainly in a paracrine manner. Although the transfection with hIFN-gamma gene also revealed a definite growth-inhibitory effect on the same tumor cells, the extent was less than that of hIFN-beta gene.
authors.affiliation: Department of Surgery, Nagoya University School of Medicine, Japan.
authors.forename: M
authors.initials: M
authors.lastname: Shimizu
authors.forename: S
authors.initials: S
authors.lastname: Akiyama
authors.forename: K
authors.initials: K
authors.lastname: Ito
authors.forename: Y
authors.initials: Y
authors.lastname: Kasai
authors.forename: H
authors.initials: H
authors.lastname: Takagi
authors.forename: M
authors.initials: M
authors.lastname: Kito
authors.forename: N
authors.initials: N
authors.lastname: Ohishi
authors.forename: K
authors.initials: K
authors.lastname: Yagi
mesh_terms: D000906:Antibodies / Q000494:pharmacology; D002455:Cell Division / Q000502:physiology; D003110:Colonic Neoplasms / Q000378:metabolism / Q000628:therapy*; D018076:DNA, Complementary / Q000008:administration & dosage* / Q000235:genetics; D015316:Genetic Therapy / Q000379:methods*; D006801:Humans; D016899:Interferon-beta / Q000096:biosynthesis / Q000235:genetics* / Q000378:metabolism; D007371:Interferon-gamma / Q000235:genetics; D008081:Liposomes; D014162:Transfection; D014407:Tumor Cells, Cultured
chemical_list: D000906:Antibodies; D018076:DNA, Complementary; D008081:Liposomes; D016899:Interferon-beta; D007371:Interferon-gamma