The effects of prostaglandin E1 and tyrosine kinase inhibitors on energy status and protein synthetic ability in hepatic ischemia-reperfusion injury.
The effects of prostaglandin E1 (PGE1) and tyrosine kinase inhibitors on hepatic energy status and protein synthesis in ischemic livers were studied using 31P-magnetic resonance spectroscopy in a rat model. The continuous administration of PGE1 significantly increased the beta-adenosine triphosphate/inorganic phosphate (beta-ATP/Pi) ratio and hepatic protein synthesis rate (HPS) after ischemia-reperfusion injury. Microscopic examination showed that the continuous administration of PGE1 inhibited the development of sinusoidal hemorrhage and edema. Thus, it was concluded that PGE1 has a beneficial effect on ischemia-reperfusion injury in the liver. Pretreatment with tyrosine kinase inhibitor also increased the beta-ATP/Pi ratio; however, when tyrosine kinase inhibitor was injected before ischemia, the HPS became significantly reduced. Based on these data, the protective effect of tyrosine kinase inhibitor is unconvincing.
Nakagohri
T
T
Second Department of Surgery, Chiba University School of Medicine, Japan.
Asano
T
T
Uematsu
T
T
Kenmochi
T
T
Kubota
T
T
Kainuma
O
O
Isono
K
K
D000527:Alprostadil / Q000494:pharmacology*; D000818:Animals; D004734:Energy Metabolism / Q000187:drug effects*; D007511:Ischemia / Q000378:metabolism*; D008099:Liver / Q000098:blood supply* / Q000378:metabolism; D009682:Magnetic Resonance Spectroscopy; D008297:Male; D010761:Phosphorus Radioisotopes; D011500:Protein Synthesis Inhibitors / Q000494:pharmacology*; D011505:Protein-Tyrosine Kinases / Q000037:antagonists & inhibitors*; D051381:Rats; D017208:Rats, Wistar; D015427:Reperfusion Injury / Q000378:metabolism*
D010761:Phosphorus Radioisotopes; D011500:Protein Synthesis Inhibitors; D011505:Protein-Tyrosine Kinases; D000527:Alprostadil