Randomly amplified polymorphic DNA PCR analysis of bovine Cryptosporidium parvum strains isolated from the watershed of the Red River of the North.
Cryptosporidium parvum is a protozoan parasite that causes the disease cryptosporidiosis in a variety of mammals, including neonatal calves and humans. Millions of oocysts are shed during acute cryptosporidiosis, and zoonotic transmission is inferred, though not proven, to be a general phenomenon. Very little is known about the degree of strain variation exhibited by bovine and human isolates, though such knowledge would enable the amount of bovine-to-human transmission to be more precisely analyzed. This research was initiated to determine whether variations exist among bovine strains isolated from a localized geographic area, the watershed of the Red River of the North. Sixteen strains were isolated and compared to each other and to two human and two calf strains from Australia by randomly amplified polymorphic DNA PCR. A statistical analysis of the data indicated that the isolates belonged to four different groups of strains.
Shianna
K V
KV
Department of Microbiology and Immunology, University of North Dakota School of Medicine, Grand Forks, North Dakota 58202, USA.
Rytter
R
R
Spanier
J G
JG
D000818:Animals; D001483:Base Sequence; D002417:Cattle / Q000469:parasitology*; D002418:Cattle Diseases / Q000469:parasitology; D003457:Cryptosporidiosis / Q000469:parasitology / Q000662:veterinary; D016785:Cryptosporidium parvum / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification*; D017931:DNA Primers / Q000235:genetics; D016054:DNA, Protozoan / Q000235:genetics*; D004197:Disease Reservoirs; D014644:Genetic Variation; D006801:Humans; D019105:Random Amplified Polymorphic DNA Technique; D015047:Zoonoses
D017931:DNA Primers; D016054:DNA, Protozoan