Residual bodies and IL-1alpha stimulate expression of mRNA for IL-1alpha and IL-1 receptor type I in cultured rat Sertoli cells.
The cytokine interleukin (IL)-1alpha may be produced both by Sertoli cells and immature male germ cells from rat and is thought to play a role in autocrine and/or paracrine regulation of the spermatogenesis. The localization of IL-1 receptors in seminiferous tubules is unknown. In this study we found a constitutive expression of IL-1 receptor type I (IL-I RI) mRNA in cultured Sertoli cells and peritubular cells from rat, whereas no such transcripts were observed in immature germ cells (pachytene spermatocytes and round spermatids). An autostimulation of IL-1alpha mRNA synthesis has previously been described in other cell types. Stimulation of Sertoli cells with recombinant IL-1alpha for 0-7 h resulted in a rapid increase in both IL-1alpha and IL-1 RI mRNA. When Sertoli cells were cultured with residual bodies for 0-48 h, mRNA levels for both IL-1alpha and IL-1 RI were increased in a biphasic manner. We suggest that phagocytosis of residual bodies triggers an autocrine IL-1alpha loop in Sertoli cells where both IL-1alpha and one of its receptors are stimulated.
Wang
J E
JE
Department of Gynecology and Obstetrics, National Hospital, University of Oslo, Norway.
Josefsen
G M
GM
Hansson
V
V
Haugen
T B
TB
D000818:Animals; D002478:Cells, Cultured; D017077:Culture Media, Conditioned; D015870:Gene Expression / Q000187:drug effects; D007375:Interleukin-1 / Q000235:genetics* / Q000494:pharmacology*; D008297:Male; D010587:Phagocytosis / Q000502:physiology; D012333:RNA, Messenger / Q000235:genetics* / Q000378:metabolism*; D051381:Rats; D017472:Receptors, Interleukin-1 / Q000145:classification / Q000235:genetics*; D011994:Recombinant Proteins / Q000494:pharmacology; D012708:Sertoli Cells / Q000187:drug effects* / Q000502:physiology*; D013091:Spermatogenesis / Q000502:physiology
D017077:Culture Media, Conditioned; D007375:Interleukin-1; D012333:RNA, Messenger; D017472:Receptors, Interleukin-1; D011994:Recombinant Proteins