Construction of a human BcgI DNA fragment library.
A library made up of 36bp DNA fragments generated by digestion of human DNA with the restriction endonuclease Bcg I has been constructed. It contains 2.5x106 independent clones, representing several times the total human genome which should contain about 400000 such fragments. It is proposed to make use of these BcgI fragments to clone part of the coding sequences contained in the minor H3 isochore which represents 3% of the human genomic DNA and a quarter of all genes.
Saupe
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ERS 155 du CNRS, Institut de Biologie, Séquences répétées et Centromères, 4 Bvd HENRI IV, 34060, Montpellier, cedex, France.
Bernard
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Laurent-Brun
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Derancourt
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Roizès
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D003001:Cloning, Molecular; D018899:CpG Islands; D018076:DNA, Complementary / Q000235:genetics; D004274:DNA, Recombinant; D015252:Deoxyribonucleases, Type II Site-Specific; D015723:Gene Library; D015894:Genome, Human; D006801:Humans; D010957:Plasmids; D013379:Substrate Specificity
D018076:DNA, Complementary; D004274:DNA, Recombinant; C073736:endodeoxyribonuclease BcgI; D015252:Deoxyribonucleases, Type II Site-Specific