title: Cyanine fluorochrome-labeled antibodies in vivo: assessment of tumor imaging using Cy3, Cy5, Cy5.5, and Cy7.
abstract: Monoclonal antibodies to two different targetable antigens were conjugated to each of four commercially available cyanine fluorochromes. Equal amounts of all four antibodies were coinjected into tumor-bearing animals and imaged. Small, superficial tumors were adequately labeled using all four fluorochromes. Large tumors were labeled well only by Cy7, probably due to self-masking and dilution effects. Cy7 was superior to other cyanine fluorochromes for visualizing structures located deep within the animal.
authors.affiliation: Center for Light Microscope Imaging and Biotechnology, Carnegie Mellon University, Pittsburgh, PA 15213, USA.
authors.forename: B
authors.initials: B
authors.lastname: Ballou
authors.forename: G W
authors.initials: GW
authors.lastname: Fisher
authors.forename: J S
authors.initials: JS
authors.lastname: Deng
authors.forename: T R
authors.initials: TR
authors.lastname: Hakala
authors.forename: M
authors.initials: M
authors.lastname: Srivastava
authors.forename: D L
authors.initials: DL
authors.lastname: Farkas
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal; D000951:Antigens, Neoplasm / Q000378:metabolism; D052160:Benzothiazoles; D002232:Carbocyanines; D005456:Fluorescent Dyes; D006801:Humans; D051379:Mice; D008807:Mice, Inbred BALB C; D010750:Phosphoproteins / Q000378:metabolism; D016601:RNA-Binding Proteins / Q000378:metabolism; D018243:Teratocarcinoma / Q000737:chemistry / Q000473:pathology; D014407:Tumor Cells, Cultured; D000097796:Nucleolin
chemical_list: D000911:Antibodies, Monoclonal; D000951:Antigens, Neoplasm; D052160:Benzothiazoles; D002232:Carbocyanines; D005456:Fluorescent Dyes; D010750:Phosphoproteins; D016601:RNA-Binding Proteins; D000097796:Nucleolin; C098793:CY5.5 cyanine dye; C089187:cyanine dye 3; C085321:cyanine dye 5; C011204:cyanine dye 7