Three-dimensional ion mobility/TOFMS analysis of electrosprayed biomolecules.
An ion mobility/mass spectrometry technique has been developed to record mass-resolved ion mobility distributions for multiple ions simultaneously. The approach involves a new instrument that couples an electrospray ion source to an injected-ion drift tube/time-of-flight mass spectrometer. Individual components in a mixture of ions are separated by mobility differences in a drift tube and subsequently dispersed by mass-to-charge ratios in a time-of-flight instrument. Flight times in the mass spectrometer are much shorter than residence times in the drift tube, making it possible to record mass-resolved ion mobilities for all ions simultaneously. The result is a three-dimensional spectrum that contains collision cross section, mass-to-charge, and ion abundance information. The instrument and data acquisition system are described. Examples of combined ion mobility/time-of-flight data are presented for distributions of electrosprayed bradykinin and ubiquitin ions.
Hoaglund
C S
CS
Department of Chemistry, Indiana University, Bloomington 47405, USA.
Valentine
S J
SJ
Sporleder
C R
CR
Reilly
J P
JP
Clemmer
D E
DE
D001920:Bradykinin / Q000032:analysis*; D013058:Mass Spectrometry / Q000295:instrumentation / Q000379:methods; D008433:Mathematics; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods; D018629:Spectrometry, Mass, Secondary Ion; D014452:Ubiquitins / Q000032:analysis*
D014452:Ubiquitins; D001920:Bradykinin