Protein profiles of Malassezia pachydermatis isolated from dogs.
The protein fractions of 30 Malassezia pachydermatis isolates from dogs (15 with otitis and 15 with dermatitis) were investigated by sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE). The electrophoresis runs resulted in bands ranging from 15.0-145.7 kDa in the ear canal secretion isolates. The values for the skin isolates varied between 16.0-141.1 kDa. The presence of protein bands occurred most frequently within the 30-40 kDa bands (66.7%), 40-50 kDa (70.0%), 50-60 kDa (73.3%) and 80-90 kDa (83.3%). No statistical differences were determined, considering the behavior of M. pachydermatis isolates from ear and skin in each band of molecular weight values, nor in the number of bands obtained.
Coutinho
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Microbiology Department, Biomedical Science Institute, Universidade de São Paulo, Brazil.
de Souza
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Paula
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D000818:Animals; D004285:Dogs; D004424:Ear Canal / Q000382:microbiology; D004591:Electrophoresis, Polyacrylamide Gel; D005656:Fungal Proteins / Q000032:analysis*; D008290:Malassezia / Q000737:chemistry*; D008565:Membrane Proteins / Q000032:analysis*; D012867:Skin / Q000382:microbiology; D018709:Statistics, Nonparametric
D005656:Fungal Proteins; D008565:Membrane Proteins