Reconstitution of the B800 bacteriochlorophylls in the peripheral light harvesting complex B800-850 of rhodobacter sphaeroides 2.4.1 with BChl a and modified (bacterio-)chlorophylls
A method is described for reversibly removing bacteriochlorophyll from the B800-site of the B850-850 antenna complex from Rhodobacter sphaeroides. This method uses the oligosaccharidic detergent Triton BG-10, together with an incubation at pH 5.0. Reconstitution at the B800-site has been successfully achieved for a range of modified bacteriochlorophylls. Copyright 1998 Elsevier Science B.V. All rights reserved.
Bandilla
M
Botanisches Institut der Universitat Munchen, Menzinger Str. 67, D-80638 Munchen, Germany.
Ucker
B
Ram
M
Simonin
I
Gelhaye
E
McDermott
G
Cogdell
RJ
Scheer
H