title: Covalent binding of carbamazepine reactive metabolites to P450 isoforms present in the skin.
abstract: Carbamazepine is an anticonvulsant associated with a high risk for severe cutaneous reactions. Upon metabolism by cytochrome P450, carbamazepine may produce reactive metabolites. We evaluated in vitro the covalent binding of carbamazepine reactive metabolites on human P450s and then the presence of these P450s in human epidermis. Carbamazepine reactive metabolites covalent binding to human liver microsomes involved P450 subfamilies 1A, 2C and 3A. Specific covalent binding to yeasts expressing different P450s showed that carbamazepine reactive metabolites bound specifically to P450 1A2 and 3A4. We confirmed the constitutive presence of P450 3A in human epidermis and after induction with coaltar of P450 1A. Consequently, the production in epidermis of carbamazepine reactive metabolites is theoretically possible with formation of P450 adduct metabolites.
authors.affiliation: Department of Dermatology, CHU Henri-Mondor, Paris XII University, Créteil, France. wolk@univ-paris12.fr
authors.forename: P
authors.initials: P
authors.lastname: Wolkenstein
authors.forename: C
authors.initials: C
authors.lastname: Tan
authors.forename: S
authors.initials: S
authors.lastname: Lecoeur
authors.forename: J
authors.initials: J
authors.lastname: Wechsler
authors.forename: N
authors.initials: N
authors.lastname: Garcia-Martin
authors.forename: D
authors.initials: D
authors.lastname: Charue
authors.forename: M
authors.initials: M
authors.lastname: Bagot
authors.forename: P
authors.initials: P
authors.lastname: Beaune
mesh_terms: D000328:Adult; D000927:Anticonvulsants / Q000378:metabolism / Q000493:pharmacokinetics / Q000633:toxicity; D002220:Carbamazepine / Q000031:analogs & derivatives* / Q000378:metabolism / Q000633:toxicity; D003577:Cytochrome P-450 Enzyme System / Q000235:genetics / Q000378:metabolism*; D005260:Female; D006801:Humans; D066298:In Vitro Techniques; D008658:Inactivation, Metabolic; D007700:Kinetics; D008297:Male; D008862:Microsomes, Liver / Q000378:metabolism; D008875:Middle Aged; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000235:genetics / Q000378:metabolism; D012867:Skin / Q000187:drug effects / Q000378:metabolism*; D013262:Stevens-Johnson Syndrome / Q000209:etiology / Q000378:metabolism
chemical_list: D000927:Anticonvulsants; D011994:Recombinant Proteins; D002220:Carbamazepine; D003577:Cytochrome P-450 Enzyme System