title: [Prothrombin time and thromboplastin time. On-site measurement of the prothrombin time and activated partial thromboplastin time of surgical patients with laser photometry].
abstract: Turnaround time for analysis of prothrombin time (PT) and activated partial thromboplastin time (APTT) by standard laboratory methods ranges between 40 min and several hours. The delay in obtaining the test results limits their clinical utility for treatment of perioperative coagulation disorders and adequate anti-coagulation therapy. In this study, we compared on-site coagulation testing (OCT) of whole blood, which takes about 3 min, with standard laboratory plasma coagulation tests by our institutional laboratory (LAB) to assess the accuracy of the OCT in a clinical setting (abdominal and postcardiac surgery).
  
  
  METHODS
  PT of 62 patients with abdominal surgery was measured intra- and postoperatively using both LAB (KC 40, Thromborel S, Centeon) and OCT (CoaguChek Plus, Boehringer Mannheim) systems. APTT was determined by LAB-(KC 40, Pathromtin, Centeon) and OCT-methods in 53 patients who underwent cardiac surgery requiring cardiopulmonary bypass.
  
  
  RESULTS
  Linear regression demonstrated a strong and significant (p = 0.0001) correlation of OCT- and LAB-determinations both for PT (r = 0.92) and APTT (r = 0.91). For PT testing, bias analyses showed an agreement between OCT- and LAB-International Normalized Ratio (INR) (bias = 0.24; relative error = 14.6%) that was considered clinically acceptable, with 95% of the INR-differences lying between -0,26 and +0,74 (mean +/- 2 SD). Although commercial APTT-reagents usually differ in their sensitivity to heparin, we also found an acceptable agreement between OCT- and LAB-APTT values (bias = 6.7 s +/- 22 s; mean +/- 2 SD; relative error = 12%).
  
  
  CONCLUSIONS
  On-site coagulation monitoring provides a rapid, convenient, and accurate assessment of coagulation that can both guide specific anti-coagulation therapy and optimize therapy control of coagulation disorders after cardiac and abdominal operations. As a consequence, OCT offers a valuable tool to reduce the inappropriate use of fresh frozen plasma and to improve cost-effectiveness.
authors.affiliation: Klinik für Anästhesiologie, Friedrich-Alexander-Universität Erlangen-Nürnberg.
authors.forename: K
authors.initials: K
authors.lastname: Tschaikowsky
authors.forename: R
authors.initials: R
authors.lastname: Balint
authors.forename: E
authors.initials: E
authors.lastname: Pscheidl
authors.forename: F
authors.initials: F
authors.lastname: Bremer
mesh_terms: D006801:Humans; D007834:Lasers; D016343:Monitoring, Intraoperative / Q000295:instrumentation*; D010314:Partial Thromboplastin Time; D010783:Photometry; D011517:Prothrombin Time; D012044:Regression Analysis
vernacular_title: Prothrombinzeit und Thromboplastinzeit. On-site Messung der Prothrombinzeit und der Aktivierten Partiellen Thromboplastinzeit bei operativen Eingriffen mittels Laserphotometrie.