Evidence for oxidatively modified lipid-protein complexes in endometrium and endometriosis.
OBJECTIVE
To evaluate for the presence of oxidatively modified lipid-protein complexes in endometriosis and endometrium of women with endometriosis and controls.


METHODS
Controlled clinical study.


METHODS
Academic tertiary care center.


METHODS
Women undergoing surgery for pelvic pain, infertility, endometriosis, or tubal ligation controls.


METHODS
Biopsy of endometrium and endometriosis.


METHODS
Staining with antibodies to oxidatively modified lipid proteins (HNE-7, MDA2), macrophages (HAM-56), and muscle cell actin (HHF-35).


RESULTS
Both endometrium and endometriosis tissues contain stromal cells that immunostain with HAM-56 and show immunostaining (both intracellular and extracellular) with HNE-7 and MDA2. Some endometriotic implants show patchy staining with HHF-35. Endometrium was devoid of staining with HHF-35. Control staining with nonimmune sera in both tissues was also devoid of staining.


CONCLUSIONS
These data strongly implicate the occurrence of oxidative stress in endometriosis tissue. These data also suggest that oxidative modification is a normal physiological process in endometrium.
Murphy
A A
AA
Department of Gynecology and Obstetrics, Emory University, Atlanta, Georgia 30322, USA.
Palinski
W
W
Rankin
S
S
Morales
A J
AJ
Parthasarathy
S
S
D000328:Adult; D004715:Endometriosis / Q000378:metabolism* / Q000473:pathology; D004717:Endometrium / Q000378:metabolism* / Q000473:pathology; D000939:Epitopes / Q000276:immunology; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D008077:Lipoproteins, LDL / Q000276:immunology / Q000378:metabolism*; D012016:Reference Values; D014018:Tissue Distribution
D000939:Epitopes; D008077:Lipoproteins, LDL; C099252:oxidized low density lipoprotein