Effect of dehydroepiandrosterone sulfate on interleukin-8 receptor during cervical ripening.
We investigated the effects of dehydroepiandrosterone sulfate (DHA-S) on the production of interleukin-8 (IL-8) and expression of the interleukin-8 receptor (IL-8 R) in human cervical tissue. DHA-S increased the levels of IL-8 in cultured human cervical fibroblasts and in the supernatant in a time- and dose-dependent manner. DHA-S induced IL-8 and IL-8 R expression in human cervical fibroblasts and human pregnant cervical tissue at term in a dose-dependent manner. In addition, it induced the expression of IL-8 R in an explant culture of human cervical tissue and cultured human cervical fibroblasts in a time- and dose-dependent manner. However, dehydroepiandrosterone (DHA) only slightly induced the production of IL-8 and the expression of IL-8 R in the same cells and tissue. These results suggest that DHA-S up-regulates the autocrine system of IL-8 through the expression of IL-8 R.
Kanayama
N
N
Department of Obstetrics and Gynecology, Hamamatsu University School of Medicine, Japan.
El Maradny
E
E
Goto
J
J
Terao
T
T
D015703:Antigens, CD / Q000187:drug effects*; D002478:Cells, Cultured; D002584:Cervix Uteri / Q000187:drug effects*; D002585:Cesarean Section; D019314:Dehydroepiandrosterone Sulfate / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D017558:Elective Surgical Procedures; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D016209:Interleukin-8 / Q000096:biosynthesis*; D011247:Pregnancy; D018123:Receptors, Interleukin / Q000187:drug effects*; D023062:Receptors, Interleukin-8A
D015703:Antigens, CD; D016209:Interleukin-8; D018123:Receptors, Interleukin; D023062:Receptors, Interleukin-8A; D019314:Dehydroepiandrosterone Sulfate