[A dynamic study of the intensity of mitogenesis in an osteoblast culture in the presence of ultrasonically dispersed hydroxyapatite].
Ceramic hydroxyapatite is frequently used in clinical practice nowadays; it is applied in surgical interventions on facial skull bones. This biological material is used as bone substitute and bone connector, but many authors claim that it is devoid of osteoinductor properties. Research is in progress on the effects of a "cold", that is, biochemically active form of ceramic hydroxyapatite on proliferative activity of a number of cell populations. No mitogenesis stimulation was observed during incubation of hydroxyapatite with osteoblast culture; on the contrary, a reduction of proliferative processes intensity was observed. Remembering that hydroxyapatite is a surfactant we repeated this experiment under similar conditions but used a hydroxyapatite molbfication with specific activity increased by two orders in comparison with its close analogs. Mitogenesis intensity was assessed by radiometric method from 3H-thymidine incorporation. The data indicated stimulation of proliferative processes in osteoblast culture.
Zuev
V P
VP
Sergeev
P V
PV
Pankratov
A S
AS
Bolotova
E N
EN
Semeĭkin
A V
AV
Shimanovskiĭ
N L
NL
D000818:Animals; D000831:Animals, Newborn; D001672:Biocompatible Materials / Q000494:pharmacology*; D002478:Cells, Cultured; D017886:Durapatite / Q000494:pharmacology*; D008934:Mitogens / Q000494:pharmacology*; D008938:Mitosis / Q000187:drug effects; D010006:Osteoblasts / Q000166:cytology / Q000187:drug effects*; D051381:Rats; D013997:Time Factors; D014465:Ultrasonics
D001672:Biocompatible Materials; D008934:Mitogens; D017886:Durapatite
Dinamicheskoe issledovanie intensivnosti mitogeneza kul'tury osteoblastov v prisutstvii gidroksiapatita ul'trazvukovoĭ dispersnosti.