Synthesis of sialyl Lewis X pentasaccharide analogue for high-throughput screening of selectin blockers.
We have developed an effective synthesis of sLe(x) pentasaccharide glycolipid analogue 2. As a part of application of sLe(x) pentasaccharide glycolipid 2 synthesized here, we have investigated the construction of a high-through-put screening system for discovery of selectin blockers. As a result, it was found that compound 2 was a useful ligand for in vitro ELISA assay and could be an important material for high-throughput screening of selectin blockers.
Kiyoi
T
T
Department of Medicinal Chemistry, Kanebo, New Drug Research Laboratories, Osaka, Japan.
Inoue
Y
Y
Ohmoto
H
H
Yoshida
M
M
Kiso
M
M
Kondo
H
H
D002236:Carbohydrate Conformation; D002240:Carbohydrate Sequence; D004797:Enzyme-Linked Immunosorbent Assay; D009682:Magnetic Resonance Spectroscopy; D008969:Molecular Sequence Data; D009844:Oligosaccharides / Q000138:chemical synthesis* / Q000737:chemistry; D019087:Selectins / Q000187:drug effects*; D000080506:Sialyl Lewis X Antigen
D009844:Oligosaccharides; D019087:Selectins; D000080506:Sialyl Lewis X Antigen