Emulation of an ELISA reader and quantitative image analysis using the computer program Digital-OD.
Here, we present a Microsoft Windows application that has been written to emulate a microplate reader. After scanning a standard 96-well microplate, color gradations in the slots can be analyzed quantitatively and be used for the estimation of enzyme kinetics, binding assays or concentration determination. In a test, series accuracy of the program was determined. We show that the returned optical densities correlated highly with the true concentrations (r > 0.97). Thus, the software is useful for a wide number of applications. Further, the program is easy to handle and is available free of charge.
Jansen
S
S
Tierarztliches Institut, Universitat Gottingen Abteilung Molekularbiologie, Germany. sjansen@gwdg.de
Kriegesmann
B
B
Brenig
B
B
D004797:Enzyme-Linked Immunosorbent Assay / Q000295:instrumentation / Q000379:methods*; D007091:Image Processing, Computer-Assisted / Q000295:instrumentation / Q000379:methods*; D015203:Reproducibility of Results; D012815:Signal Processing, Computer-Assisted; D012984:Software