Rapid and sensitive liquid chromatographic method using a conductivity detector for the determination of phytic acid in food.
An LC method was developed for the determination of phytic acid in food. The separation was carried out by gradient elution on an anion-exchange column using a conductivity detector. Earlier reversed-phase LC procedures for the quantitation of phytic acid usually required a prepurification step. The prepurification can be avoided by the separation method described in this paper. The method is sensitive and selective, and can be rapidly and easily performed. It is therefore suitable for routine determination.
Talamond
P
P
Laboratoire de Biotechnologie Microbienne Tropicale, Centre ORSTOM de Montpellier, France.
Gallon
G
G
Treche
S
S
D002852:Chromatography, Ion Exchange; D002853:Chromatography, Liquid; D004553:Electric Conductivity; D005504:Food Analysis / Q000379:methods*; D007202:Indicators and Reagents; D010833:Phytic Acid / Q000032:analysis*
D007202:Indicators and Reagents; D010833:Phytic Acid