title: Delayed and selective motor neuron death after transient spinal cord ischemia: a role of apoptosis?
abstract: OBJECTIVE
  The mechanism of spinal cord injury has been thought to be related to tissue ischemia, and spinal motor neuron cells are suggested to be vulnerable to ischemia. We hypothesized that delayed and selective motor neuron death is apoptosis.
  
  
  METHODS
  Thirty-seven Japanese domesticated white rabbits weighing 2 to 3 kg were used in this study and were divided into two subgroups: a 15-minute ischemia group and a sham control group. Animals were allowed to recover at ambient temperature and were killed at 8 hours, and 1, 2, 4, and 7 days after reperfusion (n = 3 at each time point). By means of this model, cell damage was histologically analyzed. Detection of ladders of oligonucleosomal DNA fragment was investigated with gel electrophoresis up to 7 days of the reperfusion. Immunocytochemistry, in situ terminal deoxynucleotidyl transferase-mediated deoxyuridine triphosphate-biotin nick-end labeling staining was also performed.
  
  
  RESULTS
  After 15 minutes of ischemia, most of the motor neurons showed selective cell death at 7 days of reperfusion. Typical ladders of oligonucleosomal DNA fragments were detected at 2 days of reperfusion. Immunocytochemistry showed in situ terminal deoxynucleotidyl transferase-mediated deoxyuridine triphosphate-biotin nick-end staining was detected at 2 days of reperfusion selectively in the nuclei of motor neurons.
  
  
  CONCLUSIONS
  These results suggest that delayed and selective death of the motor neuron cells after transient ischemia may not be necrotic but rather predominantly apoptotic.
authors.affiliation: Department of Thoracic and Cardiovascular Surgery, Tohoku University School of Medicine, Sendai, Japan.
authors.forename: M
authors.initials: M
authors.lastname: Sakurai
authors.forename: T
authors.initials: T
authors.lastname: Hayashi
authors.forename: K
authors.initials: K
authors.lastname: Abe
authors.forename: M
authors.initials: M
authors.lastname: Sadahiro
authors.forename: K
authors.initials: K
authors.lastname: Tabayashi
mesh_terms: D000818:Animals; D017209:Apoptosis; D004247:DNA / Q000032:analysis; D053938:DNA Fragmentation; D005500:Follow-Up Studies; D007150:Immunohistochemistry; D007511:Ischemia / Q000473:pathology* / Q000503:physiopathology; D009046:Motor Neurons / Q000473:pathology*; D009707:Nucleosomes / Q000235:genetics; D011401:Promoter Regions, Genetic / Q000235:genetics; D011817:Rabbits; D015427:Reperfusion Injury; D013116:Spinal Cord / Q000098:blood supply* / Q000503:physiopathology
chemical_list: D009707:Nucleosomes; D004247:DNA