Simultaneous genotyping of alcohol dehydrogenase 2 (ADH2) and aldehyde dehydrogenase 2 (ALDH2) loci by amplified product length polymorphism (APLP) analysis.
Genotyping of the alcohol dehydrogenase 2 (ADH2) and aldehyde dehydrogenase 2 (ALDH2) loci is important in alcohol studies. We describe a method for simultaneous genotyping of ADH2 and ALDH2 based on amplified product length polymorphism (APLP) analysis. Two polymerase chain reaction (PCR) fragments for ADH2 (57 bp, 53 bp) and two for ALDH2 (78 bp, 73 bp) are simultaneously amplified. Nine banding patterns reflecting the genotypes of the ADH2 and ALDH2 loci are clearly and unambiguously distinguished. The APLP method seems to be particularly suited for large-scale population studies of ADH2 and ALDH2 loci because it is simple and rapid.
Aoshima
T
T
Department of Forensic Medicine, Yamagata University School of Medicine, Japan.
Umetsu
K
K
Yuasa
I
I
Watanabe
G
G
Suzuki
T
T
D000426:Alcohol Dehydrogenase / Q000145:classification / Q000235:genetics*; D000444:Aldehyde Dehydrogenase / Q000145:classification / Q000235:genetics*; D000071396:Aldehyde Dehydrogenase, Mitochondrial; D002874:Chromosome Mapping; D004247:DNA / Q000032:analysis; D005787:Gene Frequency; D005838:Genotype; D006801:Humans; D016133:Polymerase Chain Reaction / Q000379:methods*; D011110:Polymorphism, Genetic
D004247:DNA; D000426:Alcohol Dehydrogenase; C096127:ALDH2 protein, human; D000444:Aldehyde Dehydrogenase; D000071396:Aldehyde Dehydrogenase, Mitochondrial