title: Effect of vindesine sulfate on the radiation-induced alterations in mouse spermatogenesis: a flow cytometric evaluation.
abstract: The effect of 0.05 mg/kg body weight of vindesine sulfate was studied on the radiation-induced changes in mouse spermatogenesis at 1, 2, 7, 14, 21, 28, 35 and 70 days post-irradiation. Vindesine administration before exposure to 0, 0.5, 1, 2 and 3 Gy gamma-irradiation resulted in an increase in the radiation-induced perturbations of mouse spermatogenesis at various post-exposure time periods studied. A significant reduction in testicular weight was observed in both DDW + irradiated and VDS + irradiated groups at various post-irradiation time periods, depending on the exposure dose. Vindesine pretreatment resulted in an enhanced killing of spermatogonial cells at day 2 post-exposure at all the exposure doses, except 3 Gy when compared to DDW + irradiated controls. Consequently, the tetraploid (4C) population declined significantly by day 14 post-irradiation followed by a severe depletion in round spermatids (1C) by day 21 post-irradiation. The dose-response relationship for 4C and 1C populations was linear-quadratic at days 14 and 21, respectively. A significant elevation was observed in HC population from days 1 to 21 depending on the exposure dose. The germ cell ratios, viz. 4C:2C, 4C:S-phase, 1C:2C and 1C:4C, showed a significant decline in the VDS + irradiated group when compared to the DDW + irradiated group at various time periods, depending on the exposure dose.
authors.affiliation: Department of Radiobiology, Kasturba Medical College, Manipal, India.
authors.forename: G C
authors.initials: GC
authors.lastname: Jagetia
authors.forename: P
authors.initials: P
authors.lastname: Jyothi
authors.forename: H
authors.initials: H
authors.lastname: Krishnamurthy
mesh_terms: D000818:Animals; D005434:Flow Cytometry; D005720:Gamma Rays; D008297:Male; D051379:Mice; D009929:Organ Size; D011833:Radiation Injuries, Experimental / Q000503:physiopathology*; D016196:S Phase / Q000187:drug effects / Q000528:radiation effects; D013090:Spermatocytes / Q000187:drug effects / Q000528:radiation effects; D013091:Spermatogenesis / Q000187:drug effects* / Q000528:radiation effects*; D013093:Spermatogonia / Q000187:drug effects / Q000528:radiation effects; D013737:Testis / Q000187:drug effects / Q000503:physiopathology / Q000528:radiation effects; D014751:Vindesine / Q000494:pharmacology*
chemical_list: D014751:Vindesine