Ischemia-induced phosphorylation and translocation of stress protein alpha B-crystallin to Z lines of myocardium.
It is becoming clear that stress proteins play a role in various aspects of postischemic myocardial recovery and that the cytoskeleton of cardiac myocytes is an important determinant for cellular survival during ischemia and energy depletion. In the present study, we addressed the question of whether the cytoskeleton-binding stress protein alpha B-crystallin may be involved in early cellular responses of rat and porcine myocardium to ischemia. Immunostaining and subcellular fractionation revealed a rapid ischemia-induced redistribution of alpha B-crystallin from a cytosolic pool to intercalated disks and Z lines of the myofibrils. This striking translocation of alpha B-crystallin from the cytosol to sites of the myofibrillar system that are known to be sensitive to ischemia-reperfusion injury was accompanied by a rapid shift of a fraction of alpha B-crystallin to a more acidic isoelectric point. This shift is caused by alpha B-crystallin phosphorylation, as identified by its augmentation in the presence of phosphatase inhibitors (vanadate, fluoride) and comigration of the acidic alpha B-crystallin form with the phosphorylated B1 form of lenticular alpha B-crystallin. In view of the chaperone-like function of alpha B-crystallin in conjunction with its high level of constitutive expression in the myocardium (1-2% of soluble protein content), we consider alpha B-crystallin an excellent candidate to play a role in early aspects of the protection of the myocardial contractile apparatus against ischemia-reperfusion injury.
Golenhofen
N
N
Institute of Anatomy, Julius-Maximilians-Universität, Würzburg, Germany.
Ness
W
W
Koob
R
R
Htun
P
P
Schaper
W
W
Drenckhahn
D
D
D000818:Animals; D003459:Crystallins / Q000378:metabolism*; D003599:Cytoskeleton / Q000378:metabolism; D006360:Heat-Shock Proteins / Q000378:metabolism*; D008297:Male; D017202:Myocardial Ischemia / Q000378:metabolism* / Q000473:pathology; D009206:Myocardium / Q000378:metabolism* / Q000473:pathology*; D010766:Phosphorylation; D051381:Rats; D017208:Rats, Wistar
D003459:Crystallins; D006360:Heat-Shock Proteins