[Modification of cell damage, caused by variable rate heating, by means of changes in osmotic pressure of the medium or using chloramphenicol].
We investigated the influence of media with different osmotic pressure and of chloramphenicol on cytotoxic effects of heating with different rate of Escherichia coli B/r and Escherichia coli Bs-1 bacteria, and Zygosaccharomyces bailii yeast cells. It was shown that the hypotonic media appreciably increased cytotoxic action of heating with different rate, and, on the contrary, the hypertonic media inhibited induction of these effects. The inhibitor of protein synthesis chloramphenicol was established not to affect the bacterial thermoresistance in the process of different heating rate. On the basis of analysis of the obtained and literature data, it is proposed that a reason of cell injury dependence of the heating rate may be the availability of dissimilar levels of osmotic homeostasis destabilization in these cells when heated with different rates.
Morozov
I I
II
Medical Radiology Research Centre, Russian Academy of Medical Sciences, Obninsk.
Petin
V G
VG
D002701:Chloramphenicol / Q000494:pharmacology*; D003470:Culture Media; D004926:Escherichia coli / Q000187:drug effects*; D006358:Hot Temperature; D009997:Osmotic Pressure; D011500:Protein Synthesis Inhibitors / Q000494:pharmacology*; D004718:Saccharomycetales / Q000187:drug effects*
D003470:Culture Media; D011500:Protein Synthesis Inhibitors; D002701:Chloramphenicol
Modificatsiia povrezhdeniĭ kletok, vyzyvaemoykh nagrevom s razlichnoĭ skorost'iu, putem izmeneniia osmoticheskogo davleniia sredy ili s pomoshch'iu khloramfenikola.