title: In vivo phosphorylation of phosphofructokinase from the bivalve mollusk Mytilus galloprovincialis.
abstract: The phosphorylation state of phosphofructokinase from the mantle tissue of the facultative anaerobe mollusk Mytilus galloprovincialis was determined by a back-phosphorylation technique. The incubation of intact mantle tissue with 8-bromoadenosine 3':5'-cyclic monophosphate increased significantly the phosphate content of phosphofructokinase, which indicates that the enzyme can be phosphorylated in vivo by endogenous cAMP-dependent protein kinase. The phosphate content of mussel phosphofructokinase changes significantly during the year, in agreement with the kinetic data that show a more active enzyme form in earlier autumn. These results suggest that cAMP-dependent phosphorylation of phosphofructokinase can be partially responsible for the observed glycolytic changes associated with the annual gametogenic cycle that takes place in the mantle tissue of the mollusk. On the contrary, no differences were observed between aerobic and 24-h hypoxic mussels with regard to the phosphorylation state and the kinetic constants of phosphofructokinase. This result is inconsistent with the hypothesis that phosphorylation of phosphofructokinase is involved in the glycolytic depression that takes place during the long-term environmental hypoxia that the mollusk can undergo.
authors.affiliation: Department of Biochemistry and Molecular Biology, Faculty of Veterinary, University of Santiago de Compostela, Lugo, Spain.
authors.forename: M
authors.initials: M
authors.lastname: Fernández
authors.forename: J
authors.initials: J
authors.lastname: Cao
authors.forename: J A
authors.initials: JA
authors.lastname: Villamarín
mesh_terms: D000818:Animals; D049872:Bivalvia / Q000201:enzymology*; D017868:Cyclic AMP-Dependent Protein Kinases / Q000378:metabolism; D006019:Glycolysis; D007700:Kinetics; D010755:Organophosphates / Q000032:analysis; D010100:Oxygen / Q000378:metabolism; D010732:Phosphofructokinase-1 / Q000737:chemistry / Q000378:metabolism*; D010766:Phosphorylation; D012621:Seasons
chemical_list: D010755:Organophosphates; D010732:Phosphofructokinase-1; D017868:Cyclic AMP-Dependent Protein Kinases; D010100:Oxygen