Refined genetic map of the obligate methylotroph Methylobacillus flagellatum.
We present a refined genetic map of the obligate methylotroph Methylobacillus flagellatum. New, Hfr (high-frequency-of-transfer) donors, and pulsed-field gel electrophoresis, were used to determine that M. flagellatum contains one approximately 3.1-Mb circular chromosome, and no plasmids. A correlation between time-of-entry units and DNA length was established. Using in vivo and in vitro cloning, and sequencing, a number of new genetic markers were identified and mapped; in addition, the nature of some of the previously mapped markers was elucidated.
Serebrijski
I
I
Institute of Genetics and Selection of Industrial Microorganisms, Moscow, Russia.
Gomelsky
M
M
Bashkirova
E
E
Chistoserdov
A
A
Tsygankov
Y D
YD
D002874:Chromosome Mapping; D002876:Chromosomes, Bacterial; D003001:Cloning, Molecular; D005798:Genes, Bacterial; D005819:Genetic Markers; D006089:Gram-Negative Anaerobic Bacteria / Q000235:genetics*; D000637:Transaminases / Q000235:genetics
D005819:Genetic Markers; D000637:Transaminases; C053430:histidinol-phosphate aminotransferase