title: Beta-adrenergic-induced cytosolic redistribution of Rap1 in rat parotid acini: role in secretion.
abstract: Rap1 has recently been identified on the secretory granule membrane and plasma membrane of rat parotid acinar cells (N. J. D'Silva, D. DiJulio, C. B. Belton, K. L. Jacobson, and E. L. Watson. J. Histochem. Cytochem. 45: 965-973, 1997). In the present study, we examined the cellular redistribution of Rap1 following treatment of acini with isoproterenol (ISO), the beta-adrenergic agonist, and determined the relationship between translocation and amylase release. In the presence of ISO, Rap1 translocated to the cytosol in a concentration- and time-dependent manner; this effect was not mimicked by the muscarinic agonist, carbachol. Translocation was maximal at 1 microM ISO and paralleled amylase release immediately after ISO stimulation. Rap1 translocation and amylase release were blocked by the beta-adrenergic antagonist, propranolol, whereas okadaic acid, a downstream secretory inhibitor, significantly blocked amylase release but did not inhibit Rap1 redistribution. Results suggest that the translocation of Rap1 is causally related to secretion and that the role of Rap1 in secretion is at a site proximal to the exocytotic event.
authors.affiliation: Department of Oral Biology, University of Washington, Seattle, Washington 98195, USA.
authors.forename: N J
authors.initials: NJ
authors.lastname: D'Silva
authors.forename: K L
authors.initials: KL
authors.lastname: Jacobson
authors.forename: S M
authors.initials: SM
authors.lastname: Ott
authors.forename: E L
authors.initials: EL
authors.lastname: Watson
mesh_terms: D000318:Adrenergic beta-Agonists / Q000494:pharmacology; D000319:Adrenergic beta-Antagonists / Q000494:pharmacology; D000681:Amylases / Q000378:metabolism*; D000818:Animals; D001692:Biological Transport; D003600:Cytosol / Q000378:metabolism*; D004791:Enzyme Inhibitors / Q000494:pharmacology; D005089:Exocytosis; D019204:GTP-Binding Proteins / Q000378:metabolism*; D007545:Isoproterenol / Q000494:pharmacology; D007700:Kinetics; D008297:Male; D019319:Okadaic Acid / Q000494:pharmacology; D010306:Parotid Gland / Q000201:enzymology* / Q000648:ultrastructure*; D010744:Phosphoric Monoester Hydrolases / Q000037:antagonists & inhibitors; D011433:Propranolol / Q000494:pharmacology; D051381:Rats; D017207:Rats, Sprague-Dawley; D011943:Receptors, Adrenergic, beta / Q000502:physiology*; D020668:rap GTP-Binding Proteins
chemical_list: D000318:Adrenergic beta-Agonists; D000319:Adrenergic beta-Antagonists; D004791:Enzyme Inhibitors; D011943:Receptors, Adrenergic, beta; D019319:Okadaic Acid; D011433:Propranolol; D010744:Phosphoric Monoester Hydrolases; D000681:Amylases; D019204:GTP-Binding Proteins; D020668:rap GTP-Binding Proteins; D007545:Isoproterenol