title: Rotavirus-specific proteins are detected in murine macrophages in both intestinal and extraintestinal lymphoid tissues.
abstract: Rotaviruses replicate in mature, villous epithelial cells of the mammalian small intestine. Although rotavirus has not been detected in plasma of infants with rotavirus-induced gastroenteritis, rotavirus particles and rotavirus genomic RNA have been detected in extraintestinal sites (e.g. cerebrospinal fluid). Using a murine rotavirus strain well adapted to growth in the small intestines of suckling mice, we found that macrophages (and to a lesser extent B cells) in gut-associated lymphoid tissue contained rotavirus-specific proteins, and that these antigen-containing cells travelled to sites distant to the intestine.
authors.affiliation: Division of Gastroenterology and Nutrition, The Children's Hospital of Philadelphia, 34th St. and Civic Center Blvd., Philadelphia, PA, 19104, USA.
authors.forename: K A
authors.initials: KA
authors.lastname: Brown
authors.forename: P A
authors.initials: PA
authors.lastname: Offit
mesh_terms: D000367:Age Factors; D000818:Animals; D000833:Animals, Suckling; D000914:Antibodies, Viral / Q000097:blood; D000956:Antigens, Viral / Q000302:isolation & purification*; D001402:B-Lymphocytes / Q000821:virology; D006224:Cricetinae; D003713:Dendritic Cells / Q000821:virology; D019084:Fluorescent Antibody Technique, Indirect; D007422:Intestines / Q000276:immunology / Q000821:virology*; D008221:Lymphoid Tissue / Q000821:virology*; D008264:Macrophages / Q000821:virology*; D008643:Mesentery / Q000821:virology*; D051379:Mice; D010581:Peyer's Patches / Q000821:virology*; D011817:Rabbits; D051381:Rats; D012401:Rotavirus / Q000276:immunology*
chemical_list: D000914:Antibodies, Viral; D000956:Antigens, Viral