title: Emulation of an ELISA reader and quantitative image analysis using the computer program Digital-OD.
abstract: Here, we present a Microsoft Windows application that has been written to emulate a microplate reader. After scanning a standard 96-well microplate, color gradations in the slots can be analyzed quantitatively and be used for the estimation of enzyme kinetics, binding assays or concentration determination. In a test, series accuracy of the program was determined. We show that the returned optical densities correlated highly with the true concentrations (r > 0.97). Thus, the software is useful for a wide number of applications. Further, the program is easy to handle and is available free of charge.
authors.affiliation: Tierarztliches Institut, Universitat Gottingen Abteilung Molekularbiologie, Germany. sjansen@gwdg.de
authors.forename: S
authors.initials: S
authors.lastname: Jansen
authors.forename: B
authors.initials: B
authors.lastname: Kriegesmann
authors.forename: B
authors.initials: B
authors.lastname: Brenig
mesh_terms: D004797:Enzyme-Linked Immunosorbent Assay / Q000295:instrumentation / Q000379:methods*; D007091:Image Processing, Computer-Assisted / Q000295:instrumentation / Q000379:methods*; D015203:Reproducibility of Results; D012815:Signal Processing, Computer-Assisted; D012984:Software