Development-dependent expression of complex-type sugar chains specific to mouse brain.
We previously detected a fucosylagalactobiantenna with a bisecting GlcNAc residue (BA-2) and one lacking the GlcNAc residue linked to the Manalpha1-3 residue of BA-2 (BA-1), which were enriched specifically in mouse brain [Shimizu, H., Ochiai, K., Ikenaka, K., Mikoshiba, K., and Hase, S. (1993) J. Biochem. 114, 334-338]. Pyridylamino sugar chains were prepared from mouse brains of various ages, and BA-1 and BA-2 were quantified after separation by HPLC. In cerebrum, BA-1 was scarcely expressed in newborn brain but gradually increased in amount during development, while expression of BA-2 reached a maximum 1 week after birth followed by a rapid decrease; in adult mice, the amount of BA-1 was almost the same as that of BA-2. In cerebellum, expression of BA-1 was lower than that of BA-2 at all stages. Glycoproteins with the BA-1 and BA-2 structures were enriched in the membrane fraction, and the glycoproteins solubilized were purified by lectin-affinity chromatography and gel filtration. The results indicated that BA-1 and BA-2 occurred in glycoproteins of more than 20 kDa in cerebellum, but most BA-1 and BA-2 were found in a 80-200 kDa fraction in cerebrum. These results show that the two brain-specific sugar chains are developmentally regulated and linked to the membrane-associated glycoproteins of subcellular organellas.
Nakakita
S
S
Department of Chemistry, Graduate School of Science, Osaka University, Toyonaka, Osaka, 560, Japan.
Natsuka
S
S
Ikenaka
K
K
Hase
S
S
D000818:Animals; D001921:Brain / Q000254:growth & development / Q000378:metabolism*; D050260:Carbohydrate Metabolism; D002241:Carbohydrates / Q000235:genetics; D002851:Chromatography, High Pressure Liquid; D018507:Gene Expression Regulation, Developmental; D051379:Mice; D008813:Mice, Inbred ICR; D009928:Organ Specificity
D002241:Carbohydrates