Tertiary structural changes in the cleft containing the ATP sensitive tryptophan and reactive thiol are consistent with pivoting of the myosin heavy chain at Gly699.
The conformation of myosin subfragment 1 (S1) in the vicinity of the ATP sensitive tryptophan (Trp510) and the highly reactive thiol (SH1), both residing in the "probe-binding" cleft at the junction of the catalytic and lever arm domains, was studied to ascertain its role in the mechanism of energy transduction and force generation. In glycerinated muscle fibers in rigor, a fluorescent probe linked to SH1 detects a strained probe-binding cleft conformation following a length transient by altering emission intensity without detectably rotating. In myosin S1 in solution, the optical activity of Trp510 senses conformation change in the probe-binding cleft caused by substrate analog trapping of S1 in various structures attainable transiently during normal energy transduction. Also in S1 in solution, the induced optical activity of a fluorescein probe linked to SH1 shows sensitivity to changing probe-binding cleft conformation caused by nucleotide binding to the S1 active site. The changes in the optical activity of Trp510 and SH1 bound fluorescein in response to nucleotide or nucleotide analog binding are interpreted structurally using the S1 crystallographic coordinates and aided by a model of energy transduction that pivots at Gly699 to change probe-binding cleft conformation and to displace the S1 lever arm as during force generation. The crystallographic structure of the probe-binding cleft in S1 resembles most the nucleotide bound conformation in the native protein. A different structure, generated by pivoting at Gly699, better resembles the native rigor conformation of the probe-binding cleft. Pivoting at Gly699 rotates probes at SH1 suggesting that length transients on fibers in rigor do not cause pivoting at Gly699 or reverse the power stroke.
Burghardt
T P
TP
Department of Biochemistry and Molecular Biology, Mayo Foundation, Rochester, Minnesota 55905, USA.
Garamszegi
S P
SP
Park
S
S
Ajtai
K
K
D000255:Adenosine Triphosphate / Q000737:chemistry*; D000818:Animals; D002942:Circular Dichroism; D005452:Fluoresceins / Q000737:chemistry; D005998:Glycine / Q000737:chemistry*; D018485:Muscle Fibers, Skeletal / Q000737:chemistry; D018995:Myosin Heavy Chains / Q000737:chemistry*; D015879:Myosin Subfragments / Q000737:chemistry; D017434:Protein Structure, Tertiary; D011817:Rabbits; D012235:Rhodamines / Q000737:chemistry; D013050:Spectrometry, Fluorescence; D013438:Sulfhydryl Compounds / Q000737:chemistry*; D014364:Tryptophan / Q000737:chemistry*
D005452:Fluoresceins; D015879:Myosin Subfragments; D012235:Rhodamines; D013438:Sulfhydryl Compounds; C015296:5-iodoacetamidofluorescein; C034996:tetramethylrhodamine iodoacetamide; D014364:Tryptophan; D000255:Adenosine Triphosphate; D018995:Myosin Heavy Chains; D005998:Glycine