Ion-channels reconstituted into lipid bilayer from human sperm plasma membrane.
Ion environment and ionic fluxes through membrane are thought to be important in the spermatozoa's maturation, capacitation, and the initiating process of gamete interaction. In this work, the membrane proteins isolated from human sperm plasma membrane were reconstituted into planar lipid bilayers via fusion, and the ion channels activities were observed under voltage clamp mode. In cis 200//trans 100 mM KCl solution, a TEA-sensitive cation-selective channel with a unit conductance of 40 pS was recorded. In a gradient of 200//100 mM NaCl solutions, a Na(+)-selective channel with a unit conductance of 26 pS was recorded. In both cases, reversal potential was about-18 mV, which is close to the predicated value of a perfect Nernst K+ or Na+ electrode. In 50//10 mM CaCl2 solution, a cation channel activity with a unit conductance of 40 pS and reversal potential of about -20 mV was usually observed. In 200//100 mM NMDG(N-methyl-D-glucamine)-Cl solution, where the cation ions were substituted with NMDG, a 30-pS anion-selective channel activity was also detected. The variety in the types of ion channels observed in human spermatozoa plasma membrane suggests that ion channels may play a range of different roles in sperm physiology and gamete interaction.
Shi
Y L
YL
Shanghai Institute of Physiology, Chinese Academy of Sciences, P.R. China. ylshi@server.shcnc.ac.cn
Ma
X H
XH
D015220:Calcium Channels / Q000502:physiology; D002462:Cell Membrane / Q000502:physiology; D018118:Chloride Channels / Q000502:physiology; D004553:Electric Conductivity; D006801:Humans; D007473:Ion Channels / Q000502:physiology*; D008051:Lipid Bilayers; D008297:Male; D015221:Potassium Channels / Q000502:physiology; D015222:Sodium Channels / Q000502:physiology; D013094:Spermatozoa / Q000502:physiology*
D015220:Calcium Channels; D018118:Chloride Channels; D007473:Ion Channels; D008051:Lipid Bilayers; D015221:Potassium Channels; D015222:Sodium Channels