title: 5D4 keratan sulfate epitope identifies a subset of ramified microglia in normal central nervous system parenchyma.
abstract: Microglia expressing keratan sulfate (KS) was studied in normal central nervous system (CNS) and in rat neonatal brain cultures. The majority of KS+ cells are ramified microglia located in the brain parenchyma; positive cells were only exceptionally found in extraparenchymal structures. KS+ cells are ubiquitous, but their density is heterogeneous throughout the CNS. Serial sections incubated with anti-KS MAb and MAb against the complement receptor type 3 (CR3) revealed a higher number of CR3+ cells and double immunofluorescence showed the presence of two microglial populations: the first expressing both KS and CR3, the second expressing only CR3. Two sets of microglial cells were found also in neonatal rat microglial cultures where only a low percentage of microglial cells expressing CR3 was also KS+. KS was not induced by microglia activation.
authors.affiliation: Centro Sclerosi Multipla, Divisione Universitaria di Neurologia, Ospedale S. Luigi, Orbassano, Italy.
authors.forename: A
authors.initials: A
authors.lastname: Bertolotto
authors.forename: C
authors.initials: C
authors.lastname: Agresti
authors.forename: A
authors.initials: A
authors.lastname: Castello
authors.forename: E
authors.initials: E
authors.lastname: Manzardo
authors.forename: A
authors.initials: A
authors.lastname: Riccio
mesh_terms: D000818:Animals; D000831:Animals, Newborn / Q000378:metabolism; D000941:Antigens / Q000032:analysis; D001921:Brain / Q000166:cytology / Q000378:metabolism; D002478:Cells, Cultured; D002490:Central Nervous System / Q000166:cytology / Q000378:metabolism*; D000939:Epitopes / Q000378:metabolism; D007632:Keratan Sulfate / Q000276:immunology* / Q000378:metabolism*; D017628:Microglia / Q000276:immunology / Q000378:metabolism*; D051381:Rats; D011916:Rats, Inbred F344; D011951:Receptors, Complement / Q000378:metabolism; D012016:Reference Values
chemical_list: D000941:Antigens; D000939:Epitopes; D011951:Receptors, Complement; D007632:Keratan Sulfate