The ubiquitin-related protein RUB1 and auxin response in Arabidopsis.
The AXR1 (auxin-resistant) protein, which has features of the ubiquitin-activating enzyme E1, is required for normal response to the plant hormone auxin in Arabidopsis thaliana. ECR1 functions together with AXR1 to activate members of the RUB/NEDD8 family of ubiquitin-related proteins. Extracts from mutant seedlings lacking AXR1 did not promote formation of the RUB-ECR1 thiolester, indicating that AXR1 is the major activity in this tissue. AXR1 was localized primarily to the nucleus of dividing and elongating cells, suggesting that the targets of RUB modification are nuclear. These results indicate that auxin response depends on RUB modification of one or more nuclear proteins.
Pozo
J C
JC
Department of Biology, Indiana University, Bloomington, IN 47405-6801, USA.
Timpte
C
C
Tan
S
S
Callis
J
J
Estelle
M
M
D000595:Amino Acid Sequence; D017360:Arabidopsis / Q000187:drug effects / Q000235:genetics / Q000378:metabolism*; D029681:Arabidopsis Proteins; D002467:Cell Nucleus / Q000378:metabolism; D004229:Dithiothreitol / Q000494:pharmacology; D015870:Gene Expression; D006133:Growth Substances; D007210:Indoleacetic Acids / Q000502:physiology*; D008025:Ligases / Q000378:metabolism; D008969:Molecular Sequence Data; D009154:Mutation; D010940:Plant Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D011993:Recombinant Fusion Proteins / Q000378:metabolism; D044764:Ubiquitin-Activating Enzymes; D044767:Ubiquitin-Protein Ligases; D014452:Ubiquitins / Q000378:metabolism
C081977:AXR1 protein, Arabidopsis; D029681:Arabidopsis Proteins; C112755:ECR1 protein, Arabidopsis; D006133:Growth Substances; D007210:Indoleacetic Acids; D010940:Plant Proteins; C513170:RUB1 protein, Arabidopsis; D011993:Recombinant Fusion Proteins; D014452:Ubiquitins; D044767:Ubiquitin-Protein Ligases; D008025:Ligases; D044764:Ubiquitin-Activating Enzymes; D004229:Dithiothreitol