title: Determination of residues of flumequine and 7-hydroxyflumequine in edible sheep tissues by liquid chromatography with fluorimetric and ultraviolet detection.
abstract: A simple, sensitive, and rapid method for simultaneous determination of residues of flumequine and its microbiologically active metabolite 7-hydroxyflumequine in 100 mg sheep edible tissues (muscle, liver, kidney, and fat) by liquid chromatography is reported. After liquid-liquid cleanup with ethyl acetate, tissue extracts were injected onto a Select B column. The 2 compounds were determined by ultraviolet and fluorimetric detection. The method was repeatable and reproducible for flumequine and 7-hydroxyflumequine in muscle, liver, kidney, and fat, with limits of detection below 2 and 3 micrograms/kg for flumequine and 7-hydroxyflumequine, respectively. Mean recoveries for flumequine were 90 +/- 7, 82 +/- 7, 89 +/- 5, and 82 +/- 6% in muscle, liver, kidney, and fat respectively. Mean recoveries for 7-hydroxyflumequine were 91 +/- 2, 90 +/- 4, 86 +/- 3, and 84 +/- 4% in muscle, liver, kidney, and fat, respectively.
authors.affiliation: Centre National d'Etudes Vétérinaires et Alimentaires, Laboratoire des Médicaments Vétérinaires, Fougères, France.
authors.forename: J M
authors.initials: JM
authors.lastname: Delmas
authors.forename: A M
authors.initials: AM
authors.lastname: Chapel
authors.forename: P
authors.initials: P
authors.lastname: Sanders
mesh_terms: D000273:Adipose Tissue / Q000737:chemistry; D000818:Animals; D000890:Anti-Infective Agents / Q000032:analysis*; D002853:Chromatography, Liquid / Q000379:methods; D004350:Drug Residues; D004355:Drug Stability; D005470:Fluorometry; D024841:Fluoroquinolones; D005506:Food Contamination; D007668:Kidney / Q000737:chemistry; D008099:Liver / Q000737:chemistry; D008460:Meat / Q000032:analysis*; D009132:Muscles / Q000737:chemistry; D011807:Quinolizines / Q000032:analysis*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D012756:Sheep; D013056:Spectrophotometry, Ultraviolet
chemical_list: D000890:Anti-Infective Agents; D024841:Fluoroquinolones; D011807:Quinolizines; C040690:7-hydroxyflumequine; C012976:flumequine