Effects of Anchusa strigosa root aqueous extract on gastric ethanol-induced ulcer in laboratory animals.
Anchusa strigosa Banks et Sol (Boraginaceae) root extracts (ASRE) were prepared by soaking the dry material in boiling water. The clear soluble extract was dried and found to be 0.238 g/g dry roots. A gastric ulcer was induced in fastened animals by oral ingestion of ethanol. Administration of 0.080 g of ASRE prior to ethanol ingestion protected the stomach of the rat from ulcer formation. The ulcer index values, expressed as a percentage of total stomach surface area affected by the ulcer, were lowered from 34.0+/-4.0 to 6.0+/-0.7 and 32.5+/-9.4 to 2.2+/-1.4 by the morphometric and the planimetric methods, respectively. Treatment of the induced ulcer in guinea pigs was achieved by oral administration of ASRE at the therapeutic dose of extract of 0.286 g/kg body weight/day for 24 days. The intraperitoneal LD50 of ASRE in mice was 0.080 g extract/kg body weight. Replacing water intake by ASRE at 75 ml of variable extract concentrations of 2.865, 3.57 and 4.284 g/l per animal per day for 90 days showed no histopathological changes in all organs of the rat. However, a clear depression effect on the central nervous system and anemia were observed particularly with extract of 3.57 g/l or more.
Disi
A M
AM
Department of Biological Sciences, Faculty of Science, University of Jordan, Amman.
Tamimi
S O
SO
Abuereish
G M
GM
D000284:Administration, Oral; D000740:Anemia / Q000139:chemically induced; D000818:Animals; D000897:Anti-Ulcer Agents / Q000008:administration & dosage / Q000627:therapeutic use*; D002490:Central Nervous System / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D000431:Ethanol; D005260:Female; D006168:Guinea Pigs; D007274:Injections, Intraperitoneal; D007597:Jordan; D007928:Lethal Dose 50; D008297:Male; D051379:Mice; D010936:Plant Extracts / Q000008:administration & dosage / Q000627:therapeutic use / Q000633:toxicity; D018517:Plant Roots; D010946:Plants, Medicinal; D051381:Rats; D013270:Stomach / Q000187:drug effects / Q000473:pathology; D013276:Stomach Ulcer / Q000139:chemically induced / Q000188:drug therapy* / Q000473:pathology
D000897:Anti-Ulcer Agents; D010936:Plant Extracts; D000431:Ethanol