Atrial natriuretic peptide increases urodilatin in the circulation.
BACKGROUND
Urodilatin is a 32-amino-acid (AA) peptide formed in the kidney.


METHODS
High-performance gel permeation chromatography and high-pressure liquid chromatography evaluation of plasma followed by sensitive urodilatin and atrial natriuretic peptide (ANP) assays revealed that urodilatin does circulate distinctly from ANP.


RESULTS
Urodilatin circulates at very low levels (i.e 9-12 pg/ml). Infusion of ANP increased the circulating concentration of urodilatin 135-fold (p < 0.001), suggesting that some of the effects of ANP may be mediated by urodilatin while long-acting natriuretic peptide, vessel dilator, and kaliuretic peptide did not affect urodilatin in healthy humans (n = 30). Only ANP decreased the renal clearance of urodilatin (60-75%, p < 0.01). Urodilatin was metabolized into peptides smaller than 5 AAs as well as excreted intact into urine.


CONCLUSIONS
Urodilatin circulates and is increased by ANP in humans.
Vesely
D L
DL
Department of Medicine, James A. Haley Veterans Hospital, and University of South Florida Health Sciences Center, Tampa 33612, USA.
Overton
R M
RM
Blankenship
M
M
McCormick
M T
MT
Schocken
D D
DD
D000328:Adult; D009320:Atrial Natriuretic Factor / Q000097:blood* / Q000737:chemistry / Q000494:pharmacology*; D002850:Chromatography, Gel; D002851:Chromatography, High Pressure Liquid; D005260:Female; D006801:Humans; D008297:Male; D010446:Peptide Fragments / Q000097:blood* / Q000737:chemistry; D011863:Radioimmunoassay
D010446:Peptide Fragments; C056452:Ularitide; D009320:Atrial Natriuretic Factor