title: Quantitation of the extracellular domain of epidermal growth factor receptor in the plasma of dioxin-exposed individuals.
abstract: Animal models suggest that dioxins have a negative effect on the level of expression of the epidermal growth factor receptor in cells. In vivo the level of expression in tissue of the epidermal growth factor receptor can be monitored by assaying for the extracellular domain in blood using an enzyme linked immunosorbent assay. We have determined the levels of the extracellular domain of the epidermal growth factor receptor in the plasma of 30 individuals: 10 with high blood dioxin levels (TEQ range = 318-673 ppt), 10 with medium blood dioxin levels (TEQ range = 16-60 ppt), and 10 with low background blood dioxin levels (TEQ range = 3-10 ppt). The levels of the epidermal growth factor receptor extracellular domain were lower in the high blood dioxin group (mean +/- SD = 45 +/- 26 fmol/ml) and the medium blood dioxin group (mean +/- SD = 41 +/- 23 fmol/ml) compared with the low blood dioxin group (mean +/- SD = 73 +/- 43 fmol/ml). These results suggest that the extracellular domain of the epidermal growth factor receptor may be a marker of the biological effect of dioxin exposure.
authors.affiliation: Division of Environmental Health Sciences, Columbia University School of Public Health, New York, NY.
authors.forename: S
authors.initials: S
authors.lastname: Smith
authors.forename: A
authors.initials: A
authors.lastname: Schecter
authors.forename: O
authors.initials: O
authors.lastname: Papke
authors.forename: T
authors.initials: T
authors.lastname: Do
authors.forename: D
authors.initials: D
authors.lastname: Coulibaly
authors.forename: P
authors.initials: P
authors.lastname: Brandt-Rauf
mesh_terms: D004147:Dioxins; D004781:Environmental Exposure; D066246:ErbB Receptors / Q000097:blood*; D006801:Humans
chemical_list: D004147:Dioxins; D066246:ErbB Receptors