Profibrinolytic properties characterize a stably transformed human endothelial cell line.
A stable immortalized venous endothelial cell (IVEC) line, obtained by transfection of human umbilical vein endothelial cells (HUVEC), retains many normal differentiated endothelial characteristics. We compared the fibrinolytic activities of IVEC and HUVEC, and observed that IVEC express a more profibrinolytic phenotype than HUVEC, since they bind and activate plasminogen more efficiently, produce more tissue plasminogen activator and urokinase-type plasminogen activator antigens, and secrete less plasminogen activator inhibitor-1 antigen both under basal conditions and after stimulation with lipopolysaccharide, phorbol ester and tumor necrosis factor. Moreover, immunostaining and Western blotting of IVEC for the plasminogen/tissue plasminogen activator receptor annexin II, as well as Northern blotting of annexin II mRNA, revealed similar patterns of surface expression in IVEC and HUVEC. Plasminogen activator inhibitor-2 is expressed similarly in both cell types. IVEC may be a useful human model for functional and pharmacological explorations and modulations of fibrinolytic system components.
Camera
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Division of Hematology/Oncology, Cornell University Medical College, New York, NY 10021, USA. Marina.Camera@unimi.it
Hajjar
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Szabo
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Vicart
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Paulin
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Weksler
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D017306:Annexin A2 / Q000378:metabolism; D002461:Cell Line, Transformed; D004730:Endothelium, Vascular / Q000166:cytology / Q000502:physiology*; D005342:Fibrinolysis / Q000502:physiology*; D006801:Humans; D011956:Receptors, Cell Surface / Q000378:metabolism; D055293:Receptors, Urokinase Plasminogen Activator; D010959:Tissue Plasminogen Activator / Q000096:biosynthesis / Q000378:metabolism; D014471:Umbilical Veins / Q000166:cytology / Q000502:physiology
D017306:Annexin A2; C524898:PLAUR protein, human; D011956:Receptors, Cell Surface; D055293:Receptors, Urokinase Plasminogen Activator; D010959:Tissue Plasminogen Activator