Escherichia coli bacteriophage T1 DNA methyltransferase appears to interact with Escherichia coli enolase.
Infection of Escherichia coli cells with bacteriophage T1 induces synthesis of a bacteriophage-specific DNA methyltransferase (M.EcoT1, EC No: 2.1.1.72) with a specificity for adenine residues in the sequence 5'-GATC-3'. Purification of M.EcoT1 allowed the determination of the coding sequence of the gene (Schneider-Scherzer et al., 1990). The peptide of the entire coding sequence was over-expressed as a histidine-hexapeptide tagged protein in E. coli. Affinity purification using a Ni2+ chelating (Ni-NTA) resin yielded a recombinant enzyme with almost the same enzymatic properties as the protein purified from T1 infected E. coli cells. Interestingly, in both purification procedures, a protein with a molecular weight of 50000 was found to copurify with M.EcoT1. The N-terminal amino acid sequence identified these proteins in both cases as E. coli enolase (EC No: 4.2.1.11).
Gassner
C
C
Institute of Biochemistry, University of Innsbruck, Austria.
Schneider-Scherzer
E
E
Lottspeich
F
F
Schweiger
M
M
Auer
B
B
D004247:DNA / Q000378:metabolism; D004926:Escherichia coli / Q000201:enzymology* / Q000821:virology; D010751:Phosphopyruvate Hydratase / Q000378:metabolism*; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000378:metabolism; D012436:S-Adenosylmethionine / Q000378:metabolism; D015265:Site-Specific DNA-Methyltransferase (Adenine-Specific) / Q000235:genetics / Q000378:metabolism*; D013604:T-Phages / Q000201:enzymology* / Q000235:genetics
D011993:Recombinant Fusion Proteins; D012436:S-Adenosylmethionine; D004247:DNA; D015265:Site-Specific DNA-Methyltransferase (Adenine-Specific); D010751:Phosphopyruvate Hydratase