Searching for a functional analogy between yeast Pso4 and bacterial RecA proteins in induced mitotic recombination.
The pso4-1 mutant of S. cerevisiae is phenotypically similar to the recA mutant of E. coli; it is sensitive to DNA cross-linking agents and defective in both recombination and mutagenesis. In this paper we have measured the effect of the recA gene expression on the frequency of mitotic crossing-over and mitotic gene conversion in response to DNA damage induced by photoactivated 8-methoxypsoralen (8-MOP + UVA), ultraviolet radiation (UV) and N-methyl-N'-nitro-N-nitrosoguanidine (MNNG). The diploid pso4-1 mutant and the repair wild type strain were transformed with the multicopy plasmid carrying the recA gene placed under the control of the ADH1 promoter. The results showed that RecA is not able to restore block in induced mitotic recombination in pso4-1 cells after DNA damaging agents used. Thus RecA protein is not able to substitute Pso4 protein in homologous mitotic recombination indicating that they have probably different functions in this process.
Vlcková
V
V
Department of Genetics, Faculty of Natural Sciences, Comenius University, Bratislava, Slovakia.
Slaninová
M
M
Morais
M A
MA
Henriques
J A
JA
Fridrichová
I
I
Brozmanová
J
J
D004249:DNA Damage; D004260:DNA Repair; D004926:Escherichia coli / Q000235:genetics*; D005656:Fungal Proteins / Q000235:genetics*; D008769:Methylnitronitrosoguanidine / Q000494:pharmacology; D008938:Mitosis; D000072260:RNA Splicing Factors; D011938:Rec A Recombinases / Q000235:genetics*; D011995:Recombination, Genetic; D012441:Saccharomyces cerevisiae / Q000235:genetics*; D029701:Saccharomyces cerevisiae Proteins; D017381:Spliceosomes; D014466:Ultraviolet Rays
D005656:Fungal Proteins; C079877:PRP19 protein, S cerevisiae; D000072260:RNA Splicing Factors; D029701:Saccharomyces cerevisiae Proteins; D008769:Methylnitronitrosoguanidine; D011938:Rec A Recombinases