title: Spontaneous apoptosis of endometrial tissue is impaired in women with endometriosis.
abstract: OBJECTIVE
  To evaluate spontaneous apoptosis in single-cell suspensions of eutopic and ectopic endometrium from women with endometriosis and in eutopic endometrium from fertile controls without endometriosis.
  
  
  METHODS
  Paired specimens of eutopic and ectopic endometrial tissue from patients with endometriosis and eutopic endometrium from controls were assessed for spontaneous apoptosis.
  
  
  METHODS
  Institute for the Study and Treatment of Endometriosis and university-based research laboratories.
  
  
  METHODS
  Fertile controls (n = 10) and women with untreated endometriosis (n = 16).
  
  
  METHODS
  None.
  
  
  METHODS
  Spontaneous apoptosis assessed with an ELISA-based cell death detection kit.
  
  
  RESULTS
  Spontaneous apoptosis (monitored by absorbance) of eutopic endometrium from patients with endometriosis and fertile controls was 0.63 +/- 0.1 and 1.43 +/- 0.11, respectively. Among patients with endometriosis, spontaneous apoptosis of ectopic endometrium was 0.26 +/- 0.06. Decreased apoptosis of ectopic versus eutopic endometrium was observed independent of cycle phase.
  
  
  CONCLUSIONS
  The susceptibility of endometrial tissue to spontaneous apoptosis is significantly lower in women with endometriosis than in fertile controls. We suggest that decreased susceptibility of endometrial tissue to apoptosis contributes to the etiology or pathogenesis of endometriosis.
authors.affiliation: Institute for the Study and Treatment of Endometriosis, Rush Medical College, Chicago, Illinois, USA. hgebel@rush.edu
authors.forename: H M
authors.initials: HM
authors.lastname: Gebel
authors.forename: D P
authors.initials: DP
authors.lastname: Braun
authors.forename: A
authors.initials: A
authors.lastname: Tambur
authors.forename: D
authors.initials: D
authors.lastname: Frame
authors.forename: N
authors.initials: N
authors.lastname: Rana
authors.forename: W P
authors.initials: WP
authors.lastname: Dmowski
mesh_terms: D017209:Apoptosis / Q000502:physiology*; D004715:Endometriosis / Q000473:pathology / Q000503:physiopathology*; D004717:Endometrium / Q000473:pathology / Q000503:physiopathology*; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006801:Humans; D012016:Reference Values