title: Pax7 includes two polymorphic homeoboxes which contain rearrangements associated with differences in the ability to regenerate damaged skeletal muscle in adult mice.
abstract: Pax7 is a paired-type homeobox gene which has previously been shown to play an important role in skeletal muscle formation. It is expressed in skeletal muscle of the limbs during embryogenesis and in adulthood. The aims of this study were firstly to determine the degree of polymorphism of Pax7 amongst inbred laboratory mice using Southern blotting and Pax7 regional specific sub-probes. Secondly, functional studies were performed on mice with each of the different structural forms of Pax7 to determine whether they were associated with differences in the ability to regenerate damaged skeletal muscle. Four different allelic forms of Pax7 have now been identified in laboratory mice indicating that the previously reported DNA sequence of Pax7 is not applicable to all laboratory mice. Hybridisation patterns of TaqI digested DNA representing each of the different Pax7 alleles with the Pax7 specific sub-probes suggested that in contrast to previous findings, Pax7 is associated with two highly polymorphic homeoboxes. The presence of two homeoboxes in BALB/c mice has been confirmed by DNA sequencing. Results of functional studies have also shown that the ability to regenerate damaged skeletal muscle in adult mice is strongly associated with the presence of a 0.15-kb TaqI fragment derived from one of the homeoboxes.
authors.affiliation: Department of Pathology, University of Western Australia, Nedlands, Australia.
authors.forename: P H
authors.initials: PH
authors.lastname: Kay
authors.forename: D
authors.initials: D
authors.lastname: Harmon
authors.forename: S
authors.initials: S
authors.lastname: Fletcher
authors.forename: T
authors.initials: T
authors.lastname: Robertson
authors.forename: M
authors.initials: M
authors.lastname: Ziman
authors.forename: J M
authors.initials: JM
authors.lastname: Papadimitriou
mesh_terms: D000483:Alleles; D000818:Animals; D001483:Base Sequence; D015139:Blotting, Southern; D004247:DNA / Q000737:chemistry*; D015252:Deoxyribonucleases, Type II Site-Specific; D005801:Genes, Homeobox; D018398:Homeodomain Proteins; D051379:Mice; D008807:Mice, Inbred BALB C; D008969:Molecular Sequence Data; D009124:Muscle Proteins / Q000737:chemistry / Q000235:genetics*; D018482:Muscle, Skeletal / Q000502:physiology*; D009419:Nerve Tissue Proteins / Q000737:chemistry / Q000235:genetics*; D009693:Nucleic Acid Hybridization; D051763:PAX7 Transcription Factor; D010446:Peptide Fragments / Q000235:genetics; D012150:Polymorphism, Restriction Fragment Length; D012038:Regeneration / Q000235:genetics*
chemical_list: D018398:Homeodomain Proteins; D009124:Muscle Proteins; D009419:Nerve Tissue Proteins; D051763:PAX7 Transcription Factor; C497322:Pax7 protein, mouse; D010446:Peptide Fragments; D004247:DNA; D015252:Deoxyribonucleases, Type II Site-Specific; C027046:TCGA-specific type II deoxyribonucleases