Effect of hexachloro-1,3-butadiene on renal carcinogenesis in male rats pretreated with N-ethyl-N-hydroxyethylnitrosamine.
Hexachloro-1,3-butadiene (HCBD) is a potent nephrotoxicant that selectively damages the straight portion (pars recta) of the proximal tubule in the rat. To determine its effects on carcinogenesis. HCBD was administered for 30 wk at a concentration of 0.1% by weight in basal diet to male Wistar rats previously given 0.1% N-ethyl-N-hydroxyethylnitrosamine (EHEN) in the drinking water for 2 wk. The combined treatment resulted in a significantly higher incidence of renal cell tumors than when EHEN was administered alone. This chronic exposure and a short course of a 0.2% HCBD diet for 3 wk caused marked increase in the numbers of bromodeoxyuridine-incorporating cells or proliferating cell nuclear antigen-positive cells in the outer stripe of the kidney. The ability of HCBD to promote EHEN-initiated renal tumorigenesis in rats thus appears to be associated intimately with linked nephropathy and subsequent cell proliferation.
Nakagawa
Y
Y
Second Department of Pathology, Nara Medical University, Japan.
Kitahori
Y
Y
Cho
M
M
Konishi
N
N
Tsumatani
K
K
Ozono
S
S
Okajima
E
E
Hirao
Y
Y
Hiasa
Y
Y
D000236:Adenoma / Q000139:chemically induced / Q000737:chemistry / Q000473:pathology; D000818:Animals; D001835:Body Weight / Q000187:drug effects; D001973:Bromodeoxyuridine; D002070:Butadienes / Q000008:administration & dosage / Q000494:pharmacology*; D002455:Cell Division; D003043:Cocarcinogenesis; D004052:Diethylnitrosamine / Q000008:administration & dosage / Q000031:analogs & derivatives*; D004334:Drug Administration Schedule; D006965:Hyperplasia / Q000139:chemically induced / Q000473:pathology; D007680:Kidney Neoplasms / Q000139:chemically induced / Q000737:chemistry / Q000473:pathology*; D007687:Kidney Tubules, Proximal / Q000737:chemistry / Q000187:drug effects / Q000473:pathology*; D008297:Male; D009929:Organ Size / Q000187:drug effects; D018809:Proliferating Cell Nuclear Antigen / Q000032:analysis; D051381:Rats; D017208:Rats, Wistar
D002070:Butadienes; D018809:Proliferating Cell Nuclear Antigen; C023845:N-ethyl-N-hydroxyethylnitrosamine; D004052:Diethylnitrosamine; C001335:hexachlorobutadiene; D001973:Bromodeoxyuridine