[The characteristics of the enzymatic amplification of locus D17S5 alleles: the need to assess the stability of the amplification profile].
Amplification behavior of one chromosome locus (D17S5), widely used in identification studies, is investigated. The effect of preferable amplification of alleles, characteristic of this locus, often leads to an erroneous conclusion about homozygosity. Our studies showed, that along with the hazard of typing false homozygotes in analysis of locus D17S5, more intricate cases with genotype distortion can occur in expert practice, which may be characterized not only by partial loss of the true alleles, but even by amplification of nonspecific (nonallele) fragments simulating the false-heterozygotic allele profile. Analysis of the stability of amplification profile helps solve this problem.
Ivanov
P L
PL
Gyské
L I
LI
D000483:Alleles; D002877:Chromosomes, Human / Q000235:genetics*; D004247:DNA / Q000302:isolation & purification; D004587:Electrophoresis, Agar Gel; D005554:Forensic Medicine; D005784:Gene Amplification / Q000235:genetics*; D005838:Genotype; D006720:Homozygote; D006801:Humans; D021141:Nucleic Acid Amplification Techniques; D016133:Polymerase Chain Reaction; D012044:Regression Analysis
D004247:DNA
Osobennosti énzimaticheskoĭ amplifikatsii alleleĭ lokusa D17S5: neobkhodimost' otsenki ustoĭchivosti amplifikatsionnogo profilia.