title: Chemical denaturation of ovalbumin abrogates the induction of oral tolerance of mouse reaginic antibody responses.
abstract: The effect of chemical denaturation of ovalbumin (OVA) on the induction of oral tolerance of reaginic antibody responses was studied. Both urea-denatured OVA (UD-OVA) and carboxymethylated UD-OVA (CM-OVA) were purified by centrifugation. When compared with OVA and UD-OVA, CM-OVA had the least sensitizing capacity and allergenicity in IgE responses to OVA. BALB/c IgE, IgG1 and IgG antibody responses were suppressed by OVA, but not by UD-OVA or CM-OVA, fed prior to sensitization with OVA, UD-OVA, or CM-OVA in alum, respectively. The priming effect of specific IgG and IgG1 antibody responses was induced by CM-OVA fed prior to sensitization with OVA or CM-OVA. The proliferation of BALB/c spleen cells and their secretion of T helper type 2 (Th2) cytokines interleukin-4 (IL-4) and IL-5 were also orally tolerized by OVA, but not by denatured OVA. Although denatured OVA is hypoallergenic, the present result indicates that denaturation of a soluble protein prevents the induction of oral tolerance of Th2 responses.
authors.affiliation: Department of Medical Research, Veterans General Hospital-Taipei, Taiwan.
authors.forename: H J
authors.initials: HJ
authors.lastname: Peng
authors.forename: Z N
authors.initials: ZN
authors.lastname: Chang
authors.forename: S Y
authors.initials: SY
authors.lastname: Lin
authors.forename: S H
authors.initials: SH
authors.lastname: Han
authors.forename: C H
authors.initials: CH
authors.lastname: Chang
mesh_terms: D000284:Administration, Oral; D000818:Animals; D000917:Antibody Formation; D002455:Cell Division; D016207:Cytokines / Q000096:biosynthesis; D005260:Female; D006967:Hypersensitivity / Q000276:immunology; D007108:Immune Tolerance / Q000276:immunology; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D010047:Ovalbumin / Q000737:chemistry* / Q000276:immunology*; D011489:Protein Denaturation / Q000276:immunology; D051381:Rats; D017207:Rats, Sprague-Dawley; D011935:Reagins / Q000276:immunology*; D013154:Spleen / Q000166:cytology; D018418:Th2 Cells / Q000378:metabolism
chemical_list: D016207:Cytokines; D011935:Reagins; D010047:Ovalbumin