title: Simultaneous variation of temperature and gradient steepness for reversed-phase high-performance liquid chromatography method development. II. The use of further changes in conditions.
abstract: The preceding paper (Part I) suggests that simply optimizing temperature and gradient steepness will often provide an adequate reversed-phase HPLC separation. In some cases, however, this procedure will prove unsuccessful, and then further method-development experiments (involving change in other separation conditions) will be required. One strategy is to change a variable other than temperature or gradient steepness, followed by re-optimization of the latter two variables. The present paper examines the application of this approach with the aid of computer simulation to several samples.
authors.affiliation: LC Resources Inc., Walnut Creek, CA 94596, USA.
authors.forename: J W
authors.initials: JW
authors.lastname: Dolan
authors.forename: L R
authors.initials: LR
authors.lastname: Snyder
authors.forename: D L
authors.initials: DL
authors.lastname: Saunders
authors.forename: L
authors.initials: L
authors.lastname: Van Heukelem
mesh_terms: D000097:Acetonitriles / Q000737:chemistry; D000179:Acrylates / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D003198:Computer Simulation; D004952:Esters / Q000737:chemistry; D056890:Eukaryota / Q000737:chemistry*; D005227:Fatty Acids / Q000032:analysis* / Q000737:chemistry; D006863:Hydrogen-Ion Concentration; D000432:Methanol / Q000737:chemistry; D008956:Models, Chemical; D009930:Organic Chemicals / Q000032:analysis* / Q000737:chemistry; D010860:Pigments, Biological / Q000032:analysis* / Q000737:chemistry; D012997:Solvents / Q000737:chemistry; D013256:Steroids / Q000032:analysis* / Q000737:chemistry; D013696:Temperature
chemical_list: D000097:Acetonitriles; D000179:Acrylates; D004952:Esters; D005227:Fatty Acids; D009930:Organic Chemicals; D010860:Pigments, Biological; D012997:Solvents; D013256:Steroids; D000432:Methanol; C032159:acetonitrile