Autoantigenic properties of some protein subunits of catalytically active complexes of human ribonuclease P.
At least six proteins co-purify with human ribonuclease P (RNase P), a tRNA processing ribonucleoprotein. Two of these proteins, Rpp30 and Rpp38, are Th autoantigens. Recombinant Rpp30 and Rpp38 are also recognized by Th sera from systemic sclerosis patients. Two of the other proteins associated with RNase P, Rpp20 and Rpp40, do not cross-react with Th sera. Polyclonal antibodies raised against all four recombinant proteins recognize the corresponding proteins associated with RNase P and precipitate active holoenzyme. Catalytically active RNase P holoenzyme can be separated from the nucleolar and mitochondrial RNA processing endoribonuclease, RNase MRP, even though these two enzymes may share some subunits.
Jarrous
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Department of Biology, Yale University, New Haven, Connecticut 06520, USA.
Eder
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PS
Guerrier-Takada
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Hoog
C
C
Altman
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S
D000595:Amino Acid Sequence; D051017:Apoptosis Regulatory Proteins; D001324:Autoantigens / Q000096:biosynthesis / Q000235:genetics / Q000276:immunology*; D001483:Base Sequence; D002352:Carrier Proteins; D003001:Cloning, Molecular; D017124:Conserved Sequence; D004722:Endoribonucleases / Q000096:biosynthesis / Q000235:genetics / Q000276:immunology*; D005057:Eukaryotic Cells; D006801:Humans; D008969:Molecular Sequence Data; D011233:Precipitin Tests; D016337:RNA, Catalytic / Q000096:biosynthesis / Q000235:genetics / Q000276:immunology*; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000276:immunology; D043262:Ribonuclease P; D012261:Ribonucleoproteins / Q000096:biosynthesis / Q000235:genetics / Q000276:immunology*
D051017:Apoptosis Regulatory Proteins; D001324:Autoantigens; D002352:Carrier Proteins; C490350:POP1 protein, human; C112706:POP7 protein, human; D016337:RNA, Catalytic; C104646:RPP30 protein, human; D011994:Recombinant Proteins; D012261:Ribonucleoproteins; D004722:Endoribonucleases; C058330:mitochondrial RNA-processing endoribonuclease; C468368:RPP14 protein, human; C104645:RPP38 protein, human; C112707:RPP40 protein, human; D043262:Ribonuclease P