title: HSP 12 is a LEA-like protein in Saccharomyces cerevisiae.
abstract: LEA group I, II and III antibodies all recognised soluble proteins present in an extract of yeast (Saccharomyces cerevisiae). The smaller protein of the two recognised by the group I antibody displayed identical migration on SDS-PAGE to the pea seed LEA group I protein against which the antibody was raised. However, the antibody failed to recognise the predominant protein present after heating the extract at 80 degrees C for 10 min. This predominant protein, which also displayed identical migration on SDS-PAGE, was purified from the supernatant of the extract heated at 80 degrees C for 10 min. Peptide sequencing after CNBr cleavage identified the isolated protein as the heat shock protein HSP 12. Despite a previous report that HSP 12 is a heat shock protein, HSP 12 was found to increase in yeast grown at 37 degrees C compared with growth at 30 degrees C. However, increased amounts of HSP 12 were present in yeast after entry into stationary phase; this was enhanced by growth in the osmolytes NaCl and mannitol.
authors.affiliation: Department of Biochemistry, University of Cape Town, Private Bag, Rondebosch, South Africa.
authors.forename: L
authors.initials: L
authors.lastname: Mtwisha
authors.forename: W
authors.initials: W
authors.lastname: Brandt
authors.forename: S
authors.initials: S
authors.lastname: McCready
authors.forename: G G
authors.initials: GG
authors.lastname: Lindsey
mesh_terms: D000595:Amino Acid Sequence; D015180:Electrophoresis, Gel, Two-Dimensional; D006360:Heat-Shock Proteins / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism*; D008969:Molecular Sequence Data; D010940:Plant Proteins / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism*; D012441:Saccharomyces cerevisiae / Q000254:growth & development / Q000378:metabolism*; D029701:Saccharomyces cerevisiae Proteins; D017386:Sequence Homology, Amino Acid
chemical_list: C112591:HSP12 protein, S cerevisiae; D006360:Heat-Shock Proteins; D010940:Plant Proteins; D029701:Saccharomyces cerevisiae Proteins; C066246:late embryogenesis abundant protein, plant