title: An enzyme-linked oligonucleotide assay.
abstract: The recent development of in vitro methods to select high-affinity ligands by combinatorial chemistry methodologies promises unique and theoretically unlimited supplies of novel therapeutic and diagnostic reagents. One such combinatorial chemistry process, systematic evolution of ligands by exponential enrichment (SELEX), allows rapid identification, from large random sequence pools, of the few oligonucleotide sequences that bind to a desired target molecule with high affinity and specificity. We describe an enzyme-linked sandwich assay that uses a SELEX-derived oligonucleotide. This assay demonstrates that these oligonucleotides can be effective and useful analytical reagents.
authors.affiliation: NeXstar Pharmaceuticals, Boulder, CO 80301, USA. ddrolet@nexstar.com
authors.forename: D W
authors.initials: DW
authors.lastname: Drolet
authors.forename: L
authors.initials: L
authors.lastname: Moon-McDermott
authors.forename: T S
authors.initials: TS
authors.lastname: Romig
mesh_terms: D000818:Animals; D019020:Directed Molecular Evolution / Q000379:methods*; D016228:Endothelial Growth Factors / Q000032:analysis* / Q000235:genetics; D004798:Enzymes / Q000737:chemistry*; D005260:Female; D006801:Humans; D008222:Lymphokines / Q000032:analysis* / Q000235:genetics; D008297:Male; D009841:Oligonucleotides / Q000737:chemistry*; D051381:Rats; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D042461:Vascular Endothelial Growth Factor A; D042442:Vascular Endothelial Growth Factors
chemical_list: D016228:Endothelial Growth Factors; D004798:Enzymes; D008222:Lymphokines; D009841:Oligonucleotides; D042461:Vascular Endothelial Growth Factor A; D042442:Vascular Endothelial Growth Factors