Mapping and serodiagnostic application of a dominant epitope within the human herpesvirus 8 ORF 65-encoded protein.
A dominant epitope within the human herpesvirus 8 (HHV8) ORF 65-encoded protein was mapped to an 8-amino-acid (aa) sequence (RKPPSGKK [aa 162 to 169]) by an amino acid replacement method. Using a 14-aa peptide (P4) encompassing this epitope as the antigen, we developed an enzyme immunoassay for HHV8 antibodies. The presence of P4 antibodies in a panel of 61 human serum specimens was highly correlated with biopsy-confirmed Kaposi's sarcoma. The homologous Epstein-Barr virus peptide derived from BFBR3-encoded protein did not interfere with the assay, suggesting that P4 is specific for HHV8.
Pau
C P
CP
Division of AIDS, STD, and TB Laboratory Research, National Center for Infectious Diseases, Centers for Disease Control and Prevention, Atlanta, Georgia 30333, USA. CHP3@CDC.GOV
Lam
L L
LL
Spira
T J
TJ
Black
J B
JB
Stewart
J A
JA
Pellett
P E
PE
Respess
R A
RA
D017088:AIDS-Related Opportunistic Infections / Q000175:diagnosis; D000595:Amino Acid Sequence; D000914:Antibodies, Viral / Q000097:blood*; D000956:Antigens, Viral / Q000235:genetics / Q000276:immunology*; D018604:Epitope Mapping; D005260:Female; D005455:Fluorescent Antibody Technique; D019288:Herpesvirus 8, Human / Q000235:genetics / Q000276:immunology*; D006801:Humans; D016056:Immunodominant Epitopes; D007124:Immunoenzyme Techniques; D008297:Male; D009842:Oligopeptides / Q000235:genetics / Q000276:immunology; D016366:Open Reading Frames / Q000235:genetics; D012514:Sarcoma, Kaposi / Q000175:diagnosis
D000914:Antibodies, Viral; D000956:Antigens, Viral; D016056:Immunodominant Epitopes; D009842:Oligopeptides