High efficiency transformation of maize (Zea mays L.) mediated by Agrobacterium tumefaciens.
Transformants of maize inbred A188 were efficiently produced from immature embryos cocultivated with Agrobacterium tumefaciens that carried "super-binary" vectors. Frequencies of transformation (independent transgenic plants/embryos) were between 5% and 30%. Almost all transformants were normal in morphology, and more than 70% were fertile. Stable integration, expression, and inheritance of the transgenes were confirmed by molecular and genetic analysis. Between one and three copies of the transgenes were integrated with little rearrangement, and the boundaries of T-DNA were similar to those in transgenic dicotyledons and rice. F1 hybrids between A188 and five other inbreds were transformed at low frequencies.
Ishida
Y
Y
Plant Breeding and Genetics Research Laboratory, Japan Tobacco Inc., Shizuoka, Japan. KYR03634@niftyserve.or.jp
Saito
H
H
Ohta
S
S
Hiei
Y
Y
Komari
T
T
Kumashiro
T
T
D016960:Agrobacterium tumefaciens / Q000502:physiology*; D001483:Base Sequence; D046508:Culture Techniques; D004269:DNA, Bacterial / Q000235:genetics; D005819:Genetic Markers; D005822:Genetic Vectors; D005838:Genotype; D008969:Molecular Sequence Data; D012639:Seeds; D014170:Transformation, Genetic; D003313:Zea mays / Q000196:embryology / Q000235:genetics*
D004269:DNA, Bacterial; D005819:Genetic Markers; C036483:T-DNA