[Molecular basis of changes in biological properties of foot and mouth disease virus of subtype A22].
Primary structure of capsid proteins and RNA polymerase of three closely related strains of foot and mouth disease virus (FMDV), subtype A22, differing by biological properties (the initial epitheliotropic strain A22 550 and its derivatives: thermoresistant myotropic A22 550/4 and thermosensitive attenuated A22 645) are compared by nucleic acid sequencing and analysis of the amino acid sequencing. The study revealed 1 substitute in VPI and 8 in RNA polymerase in the myotropic variant and 1 substitute in VP2, 2 in VP3, 13 in VP1, and 3 in RNA polymerase. Alteration of A22 550/4 tropism is probably due to a single substitution Gly 145-->Thr in the RGD site of capsid protein VP1. Analysis of the origin and biological properties of the attenuated strain A22 645 and the results of studies of the primary structure of proteins permit us to hypothesize that attenuation is polygenic, caused by adaptation to a heterologous host (continuous porcine cell culture), and can be expressed by changes in the structure of virus antireceptor providing its binding to cell receptors. Sites responsible for the reproduction of A22 FMDV at certain temperatures are presumably located in RNA polymerase.
Shcherbakov
A V
AV
Lomakina
N F
NF
Drygin
V V
VV
Lomakin
A I
AI
Perevozchikova
N A
NA
Gusev
A A
AA
D000595:Amino Acid Sequence; D005537:Aphthovirus / Q000235:genetics* / Q000502:physiology; D001483:Base Sequence; D002213:Capsid / Q000235:genetics; D036022:Capsid Proteins; D012321:DNA-Directed RNA Polymerases / Q000235:genetics; D008969:Molecular Sequence Data; D009154:Mutation
D036022:Capsid Proteins; C442501:VP1 protein, Foot-and-mouth disease virus; D012321:DNA-Directed RNA Polymerases
Izuchenie molekuliarnykh osnov izmeneniia nekotorykh biologicheskikh svoĭstv virusa iashchura podtipa A22.