Chemokines, osteopontin, ICAM-1 gene expression in cultured rat mesangial cells.
To investigate whether MCP-1, CINC, RANTES, osteopontin and ICAM-1 mRNA could be induced in cultured rat mesangial cells by interleukin-1beta(IL-1beta), tumor necrosis factor-alpha (TNF-alpha) and lipopolysaccharide (LPS), and whether MCP-1 and CINC gene expression could be modulated by dexamethasone, Northern blot assays were performed. IL-1beta induced MCP-1, CINC, RANTES and ICAM-1 gene expression in a time dependent manner. IL-1beta-induced MCP-1, CINC and ICAM-1 mRNA amount were maximal at 3 hours exposure around 14.5, 15.7, 2.2 folds increase and IL-1beta-induced RANTES mRNA at 24 hours around 2.0 folds. TNF-alpha and LPS also induced MCP-1 and ICAM-1 gene expression. TNF-alpha also induced RANTES gene expression but LPS did not. On the other hand, IL-1beta, TNF-alpha and LPS had little effect on osteopontin gene expression but fetal calf serum could increase osteopontin mRNA. Dexamethasone suppressed the IL-1beta-induced MCP-1 and CINC mRNA. These results suggest that, through these gene expressions, mesangial cells are able to communicate directly or indirectly with macrophages or neutrophils, which may lead to glomerulosclerosis.
Lee
S K
SK
Department of Internal Medicine, College of Medicine, University of Ulsan, Seoul, Korea.
Park
J Y
JY
Chung
S J
SJ
Yang
W S
WS
Kim
S B
SB
Park
S K
SK
Park
J S
JS
D000818:Animals; D002478:Cells, Cultured; D018932:Chemokine CCL2 / Q000235:genetics; D018946:Chemokine CCL5 / Q000235:genetics; D018925:Chemokines / Q000235:genetics*; D019743:Chemokines, CXC / Q000235:genetics; D002630:Chemotactic Factors / Q000235:genetics; D003907:Dexamethasone / Q000494:pharmacology; D015870:Gene Expression; D005920:Glomerular Mesangium / Q000378:metabolism*; D006133:Growth Substances / Q000235:genetics; D018799:Intercellular Adhesion Molecule-1 / Q000235:genetics*; D036341:Intercellular Signaling Peptides and Proteins; D007375:Interleukin-1 / Q000494:pharmacology; D053495:Osteopontin; D051381:Rats; D017207:Rats, Sprague-Dawley; D012795:Sialoglycoproteins / Q000235:genetics*
D018932:Chemokine CCL2; D018946:Chemokine CCL5; D018925:Chemokines; D019743:Chemokines, CXC; D002630:Chemotactic Factors; D006133:Growth Substances; D036341:Intercellular Signaling Peptides and Proteins; D007375:Interleukin-1; D012795:Sialoglycoproteins; C506897:Spp1 protein, rat; D053495:Osteopontin; D018799:Intercellular Adhesion Molecule-1; D003907:Dexamethasone