title: Propensity for macrophage apoptosis is related to the pattern of expression and function of integrin extracellular matrix receptors.
abstract: Ligation of integrins to an extracellular matrix activates signal transduction systems which produce multiple responses in different cell types. Adhesion often provides a survival signal to cells; disruption of adhesion frequently results in apoptosis. Our laboratory has utilized apoptosis-sensitive and -resistant cell lines to investigate the role of integrin expression and function in regulation of apoptosis in macrophages. Chronic exposure of murine macrophage-like RAW264.7 cells to apoptosis-inducing agents (bacterial lipopolysaccharide and interferon-gamma) resulted in the generation of a derivative cell line (RES) resistant to apoptosis. Observation of RAW and RES cultures indicated a difference in adhesion between the two cell types. The two cell lines also exhibit significant differences in expression of integrins previously characterized to be important in apoptosis.
authors.affiliation: Molecular Cardiovascular Research Center, Case Western Reserve University, School of Medicine, Cleveland, Ohio 44106, USA.
authors.forename: R
authors.initials: R
authors.lastname: Judware
authors.forename: T S
authors.initials: TS
authors.lastname: McCormick
authors.forename: S
authors.initials: S
authors.lastname: Mohr
authors.forename: J K
authors.initials: JK
authors.lastname: Yun
authors.forename: E G
authors.initials: EG
authors.lastname: Lapetina
mesh_terms: D000818:Animals; D015703:Antigens, CD / Q000378:metabolism; D017209:Apoptosis / Q000502:physiology*; D002448:Cell Adhesion / Q000502:physiology; D002460:Cell Line; D005109:Extracellular Matrix / Q000378:metabolism; D039564:Integrin alphaV; D019012:Integrin beta1 / Q000378:metabolism; D016023:Integrins / Q000378:metabolism*; D007371:Interferon-gamma / Q000494:pharmacology; D008070:Lipopolysaccharides / Q000494:pharmacology; D008264:Macrophages / Q000166:cytology* / Q000378:metabolism*; D051379:Mice; D015398:Signal Transduction
chemical_list: D015703:Antigens, CD; D039564:Integrin alphaV; D019012:Integrin beta1; D016023:Integrins; D008070:Lipopolysaccharides; D007371:Interferon-gamma