title: The early stages of the repair of adult human diaphyseal fractures.
abstract: Periosteum was obtained within 10 days of injury from the site of 17 adult tibial diaphyseal fractures during internal fixation. Osteogenic cells, non-osteogenic cells and vascular elements were identified in situ using a variety of techniques. In all cases, the periosteum was thickened with randomly distributed plaques of cartilage and bone. Cells covering newly formed bone trabeculae expressed osteocalcin. Lectin-binding revealed high vascularity. Few mast cells were observed. Macrophages and acid phosphatase positive cells, some multinucleate, were observed in abundance. These findings suggest that the repair of the adult human diaphyseal fracture is similar to that of experimental fractures in rapidity of onset, high vascularity and in bone and cartilage formation. They differ in the fact that chondrogenesis and osteogenesis appear to be simultaneous in human fractures but sequential in experimental fractures. The paucity of mast cells suggests that they probably play no significant role in the repair of the human fractures.
authors.affiliation: Glenfield Hospital, Leicester, UK.
authors.forename: O O
authors.initials: OO
authors.lastname: Oni
mesh_terms: D000135:Acid Phosphatase / Q000378:metabolism; D000293:Adolescent; D000328:Adult; D000368:Aged; D002356:Cartilage / Q000503:physiopathology; D018483:Diaphyses / Q000293:injuries; D017102:Fracture Healing; D006801:Humans; D008875:Middle Aged; D015675:Osteocalcin / Q000378:metabolism; D010012:Osteogenesis; D010521:Periosteum / Q000473:pathology; D013978:Tibial Fractures / Q000503:physiopathology*; D016212:Transforming Growth Factor beta / Q000378:metabolism
chemical_list: D016212:Transforming Growth Factor beta; D015675:Osteocalcin; D000135:Acid Phosphatase