Human platelet CD36 (GPIIIb, GPIV) binds to cholesteryl-hemisuccinate and can be purified by a simple two-step method making use of this property.
CD36, also known as GPIIIb or GPIV, is a main protein of the platelet membrane. Accumulating evidence implicates CD36 in the pathogenesis of atherosclerosis. CD36 binds to cholesteryl-hemisuccinate coupled to agarose. This binding may be of physiologic relevance since CD36 has a long extracellular hydrophobic region at its N-terminus and belongs to a family of proteins involved in lipid metabolism. This newly discovered binding property of CD36 was used to develop a rapid, efficient and gentle two-step purification method for CD36 from human platelets.
Kronenberg
A
A
Department of Internal Medicine, University of Münster, Germany.
Grahl
H
H
Kehrel
B
B
D001792:Blood Platelets / Q000378:metabolism*; D018955:CD36 Antigens / Q000737:chemistry / Q000302:isolation & purification* / Q000378:metabolism; D002788:Cholesterol Esters; D002846:Chromatography, Affinity; D006801:Humans
D018955:CD36 Antigens; D002788:Cholesterol Esters; C013440:cholesteryl succinate