Mutation screening of the dopamine D1 receptor gene in Tourette's syndrome and alcohol dependent patients.
We report a single stranded conformational polymorphism (SSCP) analysis of the coding region of the dopamine D1 receptor (DRD1) in Tourette's syndrome (n = 50) and control (n = 50) subjects. Tourette's syndrome populations with comorbidity for attention deficit-hyperactivity disorder (AD-HD) (n = 35) and obsessive compulsive disorder (OCD) (n = 30) were also screened. As a related study, we also screened patients diagnosed with alcohol dependence (n = 72). The present study discovered no DRD1 coding region mutations in any of the Tourette's syndrome or alcohol dependent patients. One silent mutation, a C for a T at Ile49, was discovered in one control subject. The non-polymorphic structure of the DRD1 gene among the Tourette's syndrome, Tourette's syndrome comorbid with AD-HD and OCD and the alcohol dependent populations screened by SSCP suggests that coding region mutations of the DRD1 gene are unlikely to contribute to the inheritance of these disorders.
Thompson
M
M
Department of Pharmacology, University of Toronto, Ontario, Canada.
Comings
D E
DE
Feder
L
L
George
S R
SR
O'Dowd
B F
BF
D000437:Alcoholism / Q000235:genetics*; D001289:Attention Deficit Disorder with Hyperactivity / Q000235:genetics; D004252:DNA Mutational Analysis; D005820:Genetic Testing; D006801:Humans; D009771:Obsessive-Compulsive Disorder / Q000235:genetics; D017354:Point Mutation; D018807:Polymorphism, Single-Stranded Conformational; D017447:Receptors, Dopamine D1 / Q000235:genetics*; D005879:Tourette Syndrome / Q000235:genetics*
D017447:Receptors, Dopamine D1