Postexposure vaccination with a virion host shutoff defective mutant reduces UV-B radiation-induced ocular herpes simplex virus shedding in mice.
A herpes simplex virus type 1 (HSV-1) recombinant (UL41NHB) deficient in the virion host shutoff (vhs) function was tested as a therapeutic vaccine in an ultraviolet (UV) light-induced mouse ocular reactivation model. Mice were infected with HSV-1 via the cornea. Following the establishment of latency by HSV-1 the mice were subsequently vaccinated intraperitoneally with one dose of UL41NHB or with uninfected cell extract. Mice were subsequently UV-irradiated to induce viral reactivation and during the 7 days post-UV irradiation, numbers of mice shedding virus were reduced from 13/23 (57%) to 3/25 (12%), and numbers of virus-positive eye swabs were reduced from 40/161 (25%) to 6/175 (3%) by the vaccine (P < 0.001). These data suggest that deletion of vhs may be a useful strategy in the development of attenuated therapeutic HSV vaccines.
Walker
J
J
Department of Ophthalmology and Visual Sciences, Washington University School of Medicine, St. Louis, MO 63110, USA.
Laycock
K A
KA
Pepose
J S
JS
Leib
D A
DA
D000818:Animals; D005123:Eye / Q000528:radiation effects / Q000821:virology; D015817:Eye Infections / Q000517:prevention & control*; D006561:Herpes Simplex / Q000517:prevention & control*; D051379:Mice; D008815:Mice, Inbred Strains; D012260:Ribonucleases; D018139:Simplexvirus / Q000235:genetics / Q000276:immunology*; D014466:Ultraviolet Rays; D014613:Vaccines, Attenuated / Q000627:therapeutic use; D014764:Viral Proteins / Q000235:genetics; D014765:Viral Vaccines / Q000627:therapeutic use*; D017201:Virus Shedding
D014613:Vaccines, Attenuated; D014764:Viral Proteins; D014765:Viral Vaccines; C070722:virion host shutoff protein, Simplexvirus; D012260:Ribonucleases