4-Piperidinopiperidine-resistant lymphoma cells were resistant to dexamethasone- and A23187-induced apoptosis.
4-Piperidinopiperidine is a side residue of CPT-11, a derivative of camptothecin. We have previously established a 4-piperidinopiperidine-resistant lymphoma cell line, 4-pp-R, which was co-resistant to CPT-11. We report here that this cell line is cross-resistant to dexamethasone and A23187 which induce apoptosis in parent RVC cells. Examination of apoptosis-related gene expression by RT-PCR showed that bcl-2 expression was greater in 4-pp-R than in RVC. p53, bax and bcl-xL were expressed at the same level in 4-pp-R and RVC cells. These results suggest that upregulation of bcl-2 in 4-pp-R cells is related to the resistance to CPT-11 as well as to A23187 or dexamethasone.
Onishi
Y
Y
Department of Biochemistry, Tokyo Dental College, Chiba, Japan.
Oguro
M
M
Kizaki
H
H
D017209:Apoptosis; D000001:Calcimycin / Q000494:pharmacology*; D002166:Camptothecin / Q000031:analogs & derivatives / Q000494:pharmacology; D053938:DNA Fragmentation / Q000187:drug effects; D003907:Dexamethasone / Q000494:pharmacology*; D004351:Drug Resistance; D006801:Humans; D000077146:Irinotecan; D008223:Lymphoma / Q000188:drug therapy*; D010880:Piperidines / Q000494:pharmacology*; D014407:Tumor Cells, Cultured
C105149:4-(1-piperidino)piperidine; D010880:Piperidines; D000001:Calcimycin; D000077146:Irinotecan; D003907:Dexamethasone; D002166:Camptothecin