title: Identification of Pneumocystis carinii f. sp. hominis gene sequences in filtered air in hospital environments.
abstract: To evaluate the risk of a nosocomial spread of Pneumocystis carinii f. sp. hominis (P. carinii hominis), air filter samples from rooms of P. carinii pneumonia (PCP) patients, adjacent corridors, and other hospital environments have been investigated for the presence of P. carinii hominis. Amplified DNA from air filters and sputum or bronchoalveolar lavage samples from the PCP patients have been genotyped with the P. carinii hominis genes of the mitochondrial large-subunit (mtLSU) rRNA and the internal transcribed spacers (ITS1 and ITS2) of the rRNA. Genotypes of the two loci were identified by direct sequencing, and for site 85 of the mtLSU locus, three allele-specific PCR assays were used. P. carinii hominis DNA was identified in the air of five of seven PCP patient rooms and in the air of two of four air filtrations from the ward corridors. The P. carinii hominis genotypes were the same in four of the five room air samples as those in the corresponding patients, suggesting a risk of person-to-person transmission of P. carinii hominis from PCP patients. Three of 16 air samples collected in infectious disease wards without the presence of PCP patients and one sample from a cardiology unit in a separate hospital building were also positive, which further strengthens the possibility of acquisition of P. carinii hominis from the environment.
authors.affiliation: Swedish Institute for Infectious Disease Control and Microbiology and Tumor Biology Center, Karolinska Institutet, Stockholm. Mats.Olsson@smi.ki.se
authors.forename: M
authors.initials: M
authors.lastname: Olsson
authors.forename: C
authors.initials: C
authors.lastname: Lidman
authors.forename: S
authors.initials: S
authors.lastname: Latouche
authors.forename: A
authors.initials: A
authors.lastname: Björkman
authors.forename: P
authors.initials: P
authors.lastname: Roux
authors.forename: E
authors.initials: E
authors.lastname: Linder
authors.forename: M
authors.initials: M
authors.lastname: Wahlgren
mesh_terms: D000391:Air Microbiology; D016902:Air Pollution, Indoor; D001992:Bronchoalveolar Lavage Fluid / Q000382:microbiology; D004271:DNA, Fungal / Q000032:analysis* / Q000235:genetics; D004272:DNA, Mitochondrial / Q000032:analysis / Q000235:genetics; D005374:Filtration; D005800:Genes, Fungal; D005838:Genotype; D006761:Hospitals; D006801:Humans; D011010:Pneumocystis / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification*; D011020:Pneumonia, Pneumocystis / Q000635:transmission; D016133:Polymerase Chain Reaction / Q000379:methods; D012331:RNA, Fungal / Q000235:genetics; D012335:RNA, Ribosomal / Q000235:genetics; D012307:Risk Factors; D017422:Sequence Analysis, DNA; D013183:Sputum / Q000382:microbiology
chemical_list: D004271:DNA, Fungal; D004272:DNA, Mitochondrial; D012331:RNA, Fungal; D012335:RNA, Ribosomal