Leukaemia inhibitory factor stimulates proliferation of prospermatogonial stem cells.
The effect of leukaemia inhibitory factor (LIF) on the proliferation of prospermatogonial stem cells was tested in vitro. Pieces of 3-day-old rat testis were cultured in the presence of a range of doses of LIF alone or in combination with 1 ng mL(-1) seminiferous growth factor (SGF). Stimulation of the proliferative activity of quiescent prospermatogonia was detected immunocytochemically with a cell proliferation kit. After 24 h culture, LIF significantly increased the percentage of labelled prospermatogonia, with the peak of activity at 20 pg mL(-1). The combination of LIF and SGF resulted in a decrease in DNA synthetic activity of the germ cells. Hence, LIF and SGF play a role in local regulation at the onset of spermatogenesis in the rat testis.
Nikolova
D B
DB
Department of Experimental Cytology, Institute of Experimental Morphology and Anthropology, Bulgarian Academy of Sciences, Sofia.
Martinova
Y S
YS
Seidensticker
M
M
Bellvé
A R
AR
D000818:Animals; D000831:Animals, Newborn; D002455:Cell Division; D002467:Cell Nucleus / Q000648:ultrastructure; D003593:Cytoplasm / Q000648:ultrastructure; D004247:DNA / Q000096:biosynthesis; D005346:Fibroblast Growth Factors / Q000494:pharmacology; D006131:Growth Inhibitors / Q000494:pharmacology*; D015850:Interleukin-6; D053605:Leukemia Inhibitory Factor; D008222:Lymphokines / Q000494:pharmacology*; D008297:Male; D051381:Rats; D017208:Rats, Wistar; D012708:Sertoli Cells / Q000378:metabolism / Q000648:ultrastructure; D013093:Spermatogonia / Q000378:metabolism / Q000648:ultrastructure*; D013234:Stem Cells / Q000378:metabolism / Q000648:ultrastructure*
D006131:Growth Inhibitors; D015850:Interleukin-6; D053605:Leukemia Inhibitory Factor; D008222:Lymphokines; D005346:Fibroblast Growth Factors; D004247:DNA