[14C-oxalate autoradiographic studies on distribution of oxalate and calcium oxalate crystal fixation in rat kidney].
To elucidate the intrarenal distribution of oxalate and to reveal the first site of calcium oxalate crystal fixation along the nephron, we carried out 14C-oxalate renal microautoradiography by the thaw mount method 30, 60 and 90 min after intravenous administration of 14C-oxalate, in normal rats and in hyperoxaluric rats fed a vitamin B6-deficient diet for 4 and 9 weeks. In normal rats, radioactivity was observed in the entire kidney area at 30 min after the injection and at 90 min there was radioactivity only in the papilla, where radioactivity appeared in the parenchyma, mamely the epithelial cells of collecting ducts and the surrounding interstitial tissue. In hyperoxaluric rats, microautoradiograms showed the spotted deposits of 14C-oxalate in both renal papilla and medulla. In 600 x magnification of the autoradiograms, most of the deposits were detected in the epithelial cells of collecting ducts and the surrounding interstitial tissue. These deposits were identified by a polarizing microscope and X-ray microanalysis as crystal deposits of calcium oxalate. These results indicated that oxalate remained for an extended period of time in the epithelial cells of collecting ducts and in the surrounding interstitial tissue in renal papilla, and the calcium oxalate crystals fixed to the same tissue was the first step in the development of calcium oxalate stones.
Ishii
K
K
Department of Urology, Osaka City University Medical School.
Sugimoto
T
T
Kamikawa
S
S
Kanazawa
T
T
Nakatani
T
T
Yamamoto
K
K
Kishimoto
T
T
Funae
Y
Y
D000818:Animals; D001345:Autoradiography; D002129:Calcium Oxalate / Q000378:metabolism*; D003460:Crystallization; D007668:Kidney / Q000378:metabolism*; D008297:Male; D010070:Oxalates / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley; D014545:Urinary Calculi / Q000378:metabolism
D010070:Oxalates; D002129:Calcium Oxalate