title: The in vitro study on genotoxic activity of adriamycin and bleomycin in cells of mice with different catalase and superoxide dismutase activity.
abstract: Genotoxic activity of adriamycin and bleomycin in embryo cells from mice differing in anti-oxidant enzymes activity was investigated. The catalase activity in cultured in vitro embryo cells of C3H mice was 2.3-fold and superoxide dismutase 2.5-fold higher than of C57BL/10 mice. For genotoxicity evaluation, the micronucleus test in vitro was used. The results obtained indicated that the frequency of micronucleated cells in untreated C3H cultures was higher than in C57BL/10 cell cultures. The increase in micronuclei formation after treatment with adriamycin and bleomycin was higher in C57BL/10 than in C3H cells as compared with micronuclei in untreated cultures. The higher frequency of micronucleated cells in treated versus untreated C57BL/10 than C3H cell cultures may be caused by lower activity of anti-oxidant enzymes in C57BL/10 cells. It may suggest that DNA damage caused by adriamycin and bleomycin resulted from action of active oxygen species.
authors.affiliation: Drug Institute, Warsaw, Poland.
authors.forename: B
authors.initials: B
authors.lastname: Chłopkiewicz
authors.forename: B
authors.initials: B
authors.lastname: Gruber
mesh_terms: D000818:Animals; D001761:Bleomycin / Q000633:toxicity*; D002374:Catalase / Q000378:metabolism*; D004317:Doxorubicin / Q000633:toxicity*; D005347:Fibroblasts / Q000187:drug effects / Q000201:enzymology; D051379:Mice; D008809:Mice, Inbred C3H; D008810:Mice, Inbred C57BL; D015162:Micronucleus Tests; D013482:Superoxide Dismutase / Q000378:metabolism*
chemical_list: D001761:Bleomycin; D004317:Doxorubicin; D002374:Catalase; D013482:Superoxide Dismutase