Salt-promoted adsorption of proteins onto amphiphilic agarose-based adsorbents. II. Effects of salt and salt concentration.
The effects of different types of salts and salt concentrations on the selectivity in the adsorption of serum proteins have been compared for the amphiphilic agarose-based adsorbents Phenyl-Sepharose, Octyl-Sepharose, butyl-agarose and mercaptopyridine-derivatized agarose. By use of multivariate analysis, the complex interrelationships for the different combined effects were evaluated. From these analyses conclusions about similarities and/or dissimilarities in the mechanisms involved in adsorption of proteins on respective adsorbent were made.
Oscarsson
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Department of Chemical Engineering, Mälardalen University, Eskilstuna, Sweden.
Kårsnäs
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D000085:Acetates / Q000737:chemistry; D000327:Adsorption; D001798:Blood Proteins / Q000737:chemistry*; D002847:Chromatography, Agarose / Q000379:methods*; D009994:Osmolar Concentration; D011189:Potassium Chloride / Q000737:chemistry; D012492:Salts / Q000737:chemistry*; D019346:Sodium Acetate / Q000737:chemistry; D012965:Sodium Chloride / Q000737:chemistry; D013431:Sulfates / Q000737:chemistry
D000085:Acetates; D001798:Blood Proteins; D012492:Salts; D013431:Sulfates; C031512:potassium sulfate; D012965:Sodium Chloride; D019346:Sodium Acetate; D011189:Potassium Chloride; C018824:ammonium acetate