[The advantage of an in situ TRAP assay for the detection of telomerase activity using bronchial washings obtained from lung cancer patients].
In most previous reports telomerase activity in lung cancer patients has been detected using tissue extracts. We have developed a semiquantitative fluorescence-based TRAP assay using fluorescence-end-labeling primers. Moreover, we also developed an in situ TRAP assay that detects telomerase activity at the cellular level. Thus, using these TRAP assays, we can detect telomerase activity in lung cancer cells obtained from bronchial washings. A high incidence of lung cancer patients with class I-III cytology had detectable telomerase activity, thus, a combination of a cell extract based. TRAP assay and an in situ TRAP assay may provide additive information to cytology for the diagnosis of lung cancer.
Yahata
N
N
First Department of Internal Medicine, Tokyo Medical College.
Ohyashiki
K
K
Kato
H
H
Toyama
K
K
D018893:Bronchoalveolar Lavage; D006801:Humans; D017403:In Situ Hybridization; D008175:Lung Neoplasms / Q000175:diagnosis*; D016133:Polymerase Chain Reaction; D017422:Sequence Analysis, DNA; D019098:Telomerase / Q000032:analysis*
D019098:Telomerase