Multiplication of Legionella pneumophila in HeLa cells in the presence of cytoskeleton and metabolic inhibitors.
A study has been carried out on the action of cytoskeleton and metabolic inhibitors on intracellular multiplication in HeLa cells of a virulent strain of Legionella pneumophila serogroup 6. The effects of the substances were separately tested on both penetration and intracellular multiplication of L. pneumophila. Only cytochalasin A and 2-deoxy-D-glucose (2dG) affected bacterial internalisation, whereas intracellular multiplication was inhibited by cytochalasins A, B, C, D and J (D being the most active) and by 2dG with a dose-response effect. The action of 2dG was counteracted by 50 mM glucose. Experiments carried out with cytochalasin D and a rhodamine-phalloidin conjugate showed the involvement of cytoskeletal elements in intracellular multiplication of Legionella; compounds acting on microtubules had no effect.
Goldoni
P
P
Istituto di Microbiologia, Università La Sapienza, Rome, Italy. goldoni@axrma, uniromal.it
Cattani
L
L
Carrara
S
S
Pastoris
M C
MC
Sinibaldi
L
L
Orsi
N
N
D019297:2,4-Dinitrophenol / Q000494:pharmacology; D000963:Antimetabolites / Q000494:pharmacology*; D015638:Cytochalasin D / Q000494:pharmacology; D003572:Cytochalasins / Q000494:pharmacology*; D003599:Cytoskeleton / Q000502:physiology*; D003847:Deoxyglucose / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D006367:HeLa Cells; D006801:Humans; D016952:Legionella pneumophila / Q000187:drug effects / Q000254:growth & development* / Q000472:pathogenicity; D008854:Microscopy, Electron; D008856:Microscopy, Fluorescence; D014750:Vincristine / Q000494:pharmacology; D014774:Virulence
D000963:Antimetabolites; D003572:Cytochalasins; D015638:Cytochalasin D; D014750:Vincristine; D003847:Deoxyglucose; D019297:2,4-Dinitrophenol