Sublethal in vitro glucose-oxygen deprivation protects cultured hippocampal neurons against a subsequent severe insult.
Rat and gerbil hippocampus exposed to a sublethal period of ischemia becomes resistant to a subsequent period of lethal ischemia induced several days later, a phenomenon referred to as ischemic preconditioning. Here we describe ischemic preconditioning induced in vitro in cultured hippocampal neurons. Mixed neuroglial hippocampal cell cultures of 14-17 DIV were exposed to a combined glucose and oxygen deprivation (GOD). Cultures subjected to 90 min, but not 60 min, of GOD showed extensive degeneration after a 1 day recovery period. An episode of 60 min of preconditioning GOD followed 1 and 2 days later by 90 min of GOD resulted in 40-60% protection. The data demonstrate that ischemic preconditioning can be mimicked in an in vitro hippocampal cell culture system.
Khaspekov
L
L
Laboratory for Experimental Brain Research, Wallenberg Neuroscience Center, Lund University Hospital, Sweden.
Shamloo
M
M
Victorov
I
I
Wieloch
T
T
D000818:Animals; D016923:Cell Death; D015687:Cell Hypoxia; D002478:Cells, Cultured; D004622:Embryo, Mammalian; D005849:Gerbillinae; D005947:Glucose / Q000494:pharmacology; D006624:Hippocampus / Q000166:cytology*; D019194:Ischemic Preconditioning; D007700:Kinetics; D051379:Mice; D008807:Mice, Inbred BALB C; D009474:Neurons / Q000166:cytology* / Q000502:physiology*; D013997:Time Factors
D005947:Glucose