title: An extremely high conservation of RNA-protein S7 interactions during prokaryotic ribosomal biogenesis.
abstract: Direct determination of RNA-protein complex structures is often facilitated by the use of thermophilic proteins; however E. coli is the most investigated system so far. A hybrid approach is to form heterologous complexes of E. coli RNA with thermophilic proteins. The rationale for this approach to RNA-protein interactions in ribosomes is based on the ability of the thermophilic protein S7 to replace a homologous counterpart in vivo. In vitro, the protein S7 of Thermus thermophilus is able to form complexes with both the minimal 16S rRNA fragment and the intercistronic region of the str operon mRNA from E. coli (Kd = 1.4 x 10(7) M and 1.1 x 10(-7) M respectively). The interaction of Thermus S7 with the E. coli intercistronic mRNA is surprising, because this region does not exist in the thermophilic str operon. It suggests a high degree of conservation of an RNA-binding site on S7.
authors.affiliation: Institute of Physico Chemical Biology of Moscow State University, Russian Federation.
authors.forename: V A
authors.initials: VA
authors.lastname: Spiridonova
authors.forename: A V
authors.initials: AV
authors.lastname: Golovin
authors.lastname: Drygin DYu
authors.forename: A M
authors.initials: AM
authors.lastname: Kopylov
mesh_terms: D001426:Bacterial Proteins / Q000378:metabolism*; D001665:Binding Sites; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism; D007700:Kinetics; D009876:Operon / Q000502:physiology; D012329:RNA, Bacterial / Q000378:metabolism*; D012333:RNA, Messenger / Q000378:metabolism*; D012336:RNA, Ribosomal, 16S / Q000378:metabolism*; D012091:Repetitive Sequences, Nucleic Acid; D012269:Ribosomal Proteins / Q000378:metabolism*; D012270:Ribosomes / Q000378:metabolism*; D016963:Thermus thermophilus / Q000235:genetics / Q000378:metabolism
chemical_list: D001426:Bacterial Proteins; D012329:RNA, Bacterial; D012333:RNA, Messenger; D012336:RNA, Ribosomal, 16S; D012269:Ribosomal Proteins; C037442:ribosomal protein S7