Glucose and methionine uptake by rat brain tumor treated with prodrug-activated gene therapy.
The effect of acyclovir (ACV) on the metabolism of rat 9L-gliosarcoma cells expressing the herpes simplex virus-thymidine kinase gene was studied using 2-deoxy-2-[18F]fluoro-D-glucose (FDG) and L-[methyl-11C]methionine. Though the average weight of the tumors treated with ACV was significantly lower than that of the saline-injected control group, FDG and methionine uptake per weight of tumor tissue was not different between the two groups. This result exhibits a striking contrast to the metabolic pattern observed after radiation therapy, suggesting the different pathways regarding tumor cell death between the therapies.
Namba
H
H
Division of Neurological Surgery, Chiba Cancer Center, Japan. hnamba@chiba-cc.pref.chiba.jp
Iwadate
Y
Y
Iyo
M
M
Fukushi
K
K
Irie
T
T
Sueyoshi
K
K
Tagawa
M
M
Sakiyama
S
S
D000212:Acyclovir / Q000627:therapeutic use*; D000818:Animals; D000998:Antiviral Agents / Q000627:therapeutic use; D001692:Biological Transport; D001932:Brain Neoplasms / Q000378:metabolism / Q000473:pathology / Q000628:therapy*; D002250:Carbon Radioisotopes / Q000493:pharmacokinetics; D019788:Fluorodeoxyglucose F18 / Q000493:pharmacokinetics*; D015316:Genetic Therapy; D018316:Gliosarcoma / Q000378:metabolism / Q000473:pathology / Q000628:therapy*; D005947:Glucose / Q000378:metabolism*; D008297:Male; D008715:Methionine / Q000378:metabolism*; D011355:Prodrugs / Q000627:therapeutic use; D019275:Radiopharmaceuticals / Q000493:pharmacokinetics; D051381:Rats; D011916:Rats, Inbred F344; D018139:Simplexvirus / Q000201:enzymology / Q000235:genetics; D013937:Thymidine Kinase / Q000096:biosynthesis / Q000235:genetics
D000998:Antiviral Agents; D002250:Carbon Radioisotopes; D011355:Prodrugs; D019275:Radiopharmaceuticals; D019788:Fluorodeoxyglucose F18; D008715:Methionine; D013937:Thymidine Kinase; D005947:Glucose; D000212:Acyclovir