Requirement for membrane lipid peroxidation in HIV-1 gp120-induced neuroblastoma cell death.
The HIV-1 envelope protein gp120 engenders Ca(2+)-mediated, excitotoxic damage of rodent neuronal and human neuroblastoma cells in culture. Here we report that human CHP100 neuroblastoma cells undergo early peroxidation of membrane lipids following a brief exposure to gp120. This effect is prevented by preincubating cell cultures with the 21-aminosteroid U-74389G, an inhibitor of membrane lipid peroxidation, which also rescues neuroblastoma cultures from gp120-induced cell death; conversely, no protection from cell death is observed when the 21-aminosteroid is added to neuroblastoma cultures after the induction of membrane lipid peroxidation by gp120. These data indicate that membrane lipid peroxidation has a causative role in the expression of cell death produced by the viral protein.
Corasaniti
M T
MT
Faculty of Pharmacy, University of Catanzaro, Italy.
Navarra
M
M
Nisticò
S
S
Rotiroti
D
D
Maccarrone
M
M
Melino
G
G
Finazzi-Agrò
A
A
D000975:Antioxidants / Q000494:pharmacology; D002118:Calcium / Q000378:metabolism; D016923:Cell Death; D015699:HIV Envelope Protein gp120 / Q000633:toxicity*; D015497:HIV-1; D006801:Humans; D015227:Lipid Peroxidation; D008563:Membrane Lipids / Q000378:metabolism*; D009447:Neuroblastoma; D009474:Neurons / Q000187:drug effects*; D011281:Pregnatrienes / Q000494:pharmacology; D016194:Receptors, N-Methyl-D-Aspartate / Q000378:metabolism; D014407:Tumor Cells, Cultured
D000975:Antioxidants; D015699:HIV Envelope Protein gp120; D008563:Membrane Lipids; D011281:Pregnatrienes; D016194:Receptors, N-Methyl-D-Aspartate; C074965:U 74389F; D002118:Calcium