Comparison of the kinetics of pancreatic secretion and gut regulatory peptides in the plasma of preruminant calves fed milk or soybean protein.
Exocrine secretion from the pancreas and concentrations of cholecystokinin, gastrin, secretin, and somatostatin in plasma were measured in relation to feeding in 70- to 120-d-old preruminant calves fed either a milk diet or a soybean diet. Pancreatic fluid was continuously collected, measured, and reintroduced in catheterized calves. Blood samples were withdrawn for measurements of gut regulatory peptide concentrations in plasma. A slight increase in outflow of pancreatic fluid was observed 30 min before the milk diet was introduced but not before the soybean diet was fed. In contrast, concentrations and outflows of protein and trypsin immediately after feeding were higher when calves were fed the soybean diet. Overall, during the first 5 h postfeeding, the outflow of pancreatic fluid was 40% higher when the milk diet was fed than when the soybean diet was fed. No difference in outflow of protein was observed, but that of trypsin was 82% higher when the soybean diet was fed. This enhanced enzyme secretion could have been related to the increased plasma concentrations of gastrin and cholecystokinin after the soybean diet was fed. Secretin release was less in calves fed the milk diet that in calves fed the soybean diet during the first 2 h postfeeding, suggesting that this gut peptide along with gastrin and cholecystokinin, contributed to the stimulation of enzyme secretion. Plasma gut regulatory peptides could be influenced by the soybean diet, which does not coagulate in the stomach, inducing faster gastric emptying of protein and fat, and by the chemical form of protein from the soybean diet and the lower susceptibility of these proteins to protease compared with casein. However, the resulting enhancement of pancreatic trypsin secretion and activity seemed to be insufficient to increase the digestibility of soybean protein up to a level similar to that of milk.
Le Drean
G
G
Institut National de Recherche Agronomique, Laboratoire du Jeune Ruminant, Rennes, France.
Le Huerou-Luron
I
I
Gestin
M
M
Rome
V
V
Plodari
M
M
Bernard
C
C
Chayvialle
J A
JA
Guilloteau
P
P
D000821:Animal Feed; D000818:Animals; D002417:Cattle / Q000502:physiology*; D002766:Cholecystokinin / Q000097:blood; D005755:Gastrins / Q000097:blood; D005768:Gastrointestinal Hormones / Q000097:blood*; D007700:Kinetics; D008297:Male; D008892:Milk; D010179:Pancreas / Q000378:metabolism*; D010189:Pancreatic Juice / Q000378:metabolism; D012633:Secretin / Q000097:blood; D013004:Somatostatin / Q000097:blood; D030262:Soybean Proteins / Q000008:administration & dosage*; D014357:Trypsin / Q000378:metabolism
D005755:Gastrins; D005768:Gastrointestinal Hormones; D030262:Soybean Proteins; D012633:Secretin; D013004:Somatostatin; D002766:Cholecystokinin; D014357:Trypsin