Ca2+-ions stimulate DNA binding specificity of Cfr10I restriction enzyme.
The Cfr10I restriction enzyme recognizes the degenerate hexanucleotide sequence 5'-Pu/CCGGPy-3' and cleaves it as indicated. DNA binding studies of Cfr10I endonuclease were performed using gel mobility shift assay. Analysis of Cfr10I binding to DNA revealed that in the absence of metal ions Cfr10I binds to DNA containing or lacking the recognition sequence with similar low affinity. Addition of Ca2+ to the binding buffer stimulated manifestation of DNA binding specificity of Cfr10I.
Skirgaila
R
R
Institute of Biotechnology, Vilnius, Lithuania.
Siksnys
V
V
D002118:Calcium / Q000378:metabolism*; D002413:Cations, Divalent; D004247:DNA / Q000378:metabolism*; D015252:Deoxyribonucleases, Type II Site-Specific / Q000378:metabolism*; D013379:Substrate Specificity
D002413:Cations, Divalent; D004247:DNA; C066244:endodeoxyribonuclease Cfr10I; D015252:Deoxyribonucleases, Type II Site-Specific; D002118:Calcium