New mathematical approach for the evaluation of drug binding to human serum albumin by high-performance liquid affinity chromatography.
A novel mathematical approach for investigation of drug-human serum albumin (HSA) interactions by means of high-performance liquid affinity chromatography is developed. The model is based on the assumption that two types of competitive binding sites exist on the HSA molecule. The widely used single-site binding equation is extended and a proper mathematical analysis is proposed allowing the determination of the major parameters characterizing the multisite binding (cobinding) process. The utility of the new approach is proved by competitive studies on HSA binding of two model drugs, diazepam and diclofenac.
Zhivkova
Z
Z
Department of Chemistry, Faculty of Pharmacy, Medical University, Solia, Bulgaria.
Russeva
V
V
D014151:Anti-Anxiety Agents / Q000097:blood; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000097:blood; D001665:Binding Sites; D001667:Binding, Competitive; D002846:Chromatography, Affinity; D002851:Chromatography, High Pressure Liquid; D003975:Diazepam / Q000097:blood; D004008:Diclofenac / Q000097:blood; D006801:Humans; D008962:Models, Theoretical; D004364:Pharmaceutical Preparations / Q000378:metabolism*; D011485:Protein Binding; D012709:Serum Albumin / Q000378:metabolism*
D014151:Anti-Anxiety Agents; D000894:Anti-Inflammatory Agents, Non-Steroidal; D004364:Pharmaceutical Preparations; D012709:Serum Albumin; D004008:Diclofenac; D003975:Diazepam