Met-195 of the cholecystokinin-A receptor interacts with the sulfated tyrosine of cholecystokinin and is crucial for receptor transition to high affinity state.
Sulfation of the tyrosine at the seventh position from the C terminus of cholecystokinin (CCK) is crucial for CCK binding to the CCK-A receptor. Using three-dimensional modeling, we identified methionine 195 of the CCK-A receptor as a putative amino acid in interaction with the aromatic ring of the sulfated tyrosine of CCK. We analyzed the role played by the two partners of this interaction. The exchange of Met-195 for a leucine caused a minor decrease (2. 8-fold) on the affinity of the high affinity sites for sulfated CCK-9, a strong drop (73%) of their number, and a 30-fold decrease on the affinity of the low and very low affinity sites for sulfated CCK-9, with no change in their number. The mutation also caused a 54-fold decrease of the potency of the receptor to induce inositol phosphates production. The high affinity sites of the wild-type CCK-A receptor were highly selective (800-fold) toward sulfated versus nonsulfated CCK, whereas low and very low affinity sites were poorly selective (10- and 18-fold). In addition, the M195L mutant bound, and responded to, sulfated CCK analogues with decreased affinities and potencies, whereas it bound and responded to nonsulfated CCK identically to the wild-type receptor. Thus, Met-195 interacts with the aromatic ring of the sulfated tyrosine to correctly position the sulfated group of CCK in the binding site of the receptor. This interaction is essential for CCK-dependent transition of the CCK-A receptor to a high affinity state. Our data should represent an important step toward the identification of the residue(s) of the receptor in interaction with the sulfate moiety of CCK and the understanding of the molecular mechanisms that govern CCK-A receptor activation.
Gigoux
V
V
INSERM U151, Institut Louis Bugnard, CHU de Rangueil, Bat. L3, 31403 Toulouse Cedex, France.
Escrieut
C
C
Silvente-Poirot
S
S
Maigret
B
B
Gouilleux
L
L
Fehrentz
J A
JA
Gully
D
D
Moroder
L
L
Vaysse
N
N
Fourmy
D
D
D000818:Animals; D001665:Binding Sites / Q000502:physiology; D001667:Binding, Competitive; D019556:COS Cells; D002766:Cholecystokinin / Q000378:metabolism*; D007295:Inositol Phosphates / Q000032:analysis; D008024:Ligands; D008715:Methionine / Q000378:metabolism*; D008958:Models, Molecular; D015394:Molecular Structure; D016297:Mutagenesis, Site-Directed / Q000235:genetics; D011485:Protein Binding / Q000502:physiology; D043886:Receptor, Cholecystokinin A; D011949:Receptors, Cholecystokinin / Q000737:chemistry*; D014162:Transfection / Q000235:genetics; D014443:Tyrosine / Q000031:analogs & derivatives* / Q000378:metabolism
D007295:Inositol Phosphates; D008024:Ligands; D043886:Receptor, Cholecystokinin A; D011949:Receptors, Cholecystokinin; C005662:tyrosine O-sulfate; D014443:Tyrosine; D002766:Cholecystokinin; D008715:Methionine