Effect of growth hormone treatment on postprandial protein metabolism in growth hormone-deficient adults.
Growth hormone (GH) treatment of GH-deficient adults increases lean body mass. To investigate this anabolic effect of GH, body composition and postabsorptive and postprandial protein metabolism were measured in 12 GH-deficient adults randomized to placebo or GH treatment. Protein metabolism was measured after an infusion of [1-13C]leucine before and after a standard meal at 0 and 2 mo. After 2 mo, there was an increase in lean body mass in the GH group (P < 0. 05) but no change in the placebo group. In the postabsorptive state, there was increased nonoxidative leucine disappearance (NOLD; a measure of protein synthesis) and leucine metabolic clearance rate and decreased leucine oxidation in the GH group (P < 0.05) but no change in the placebo group. After the meal, there was an increase in NOLD and oxidation in all studies (P < 0.05), but the increase in NOLD, measured as area under the curve, was greater in the GH group (P < 0.05). This study clearly demonstrates for the first time that the increase in protein synthesis in the postabsorptive state after GH treatment of GH-deficient adults is maintained in the postprandial state.
Russell-Jones
D L
DL
Department of Endocrinology, United Medical and Dental Schools, St. Thomas' Campus, London SE1 7EH, United Kingdom.
Bowes
S B
SB
Rees
S E
SE
Jackson
N C
NC
Weissberger
A J
AJ
Hovorka
R
R
Sonksen
P H
PH
Umpleby
A M
AM
D000328:Adult; D001823:Body Composition; D002096:C-Peptide / Q000097:blood; D002247:Carbon Isotopes; D005230:Fatty Acids, Nonesterified / Q000097:blood; D005260:Female; D005502:Food; D019382:Human Growth Hormone / Q000172:deficiency* / Q000494:pharmacology* / Q000627:therapeutic use; D006801:Humans; D007334:Insulin-Like Growth Factor I / Q000378:metabolism; D007930:Leucine; D008297:Male; D008657:Metabolic Clearance Rate; D008875:Middle Aged; D010919:Placebos; D014176:Protein Biosynthesis
D002096:C-Peptide; D002247:Carbon Isotopes; D005230:Fatty Acids, Nonesterified; D010919:Placebos; D019382:Human Growth Hormone; D007334:Insulin-Like Growth Factor I; D007930:Leucine