The effect of photomirex on the in vitro perfused ovary of the rat.
Photomirex, a photodegradation product of the insecticide mirex, is an environmental contaminant that has been identified in Great Lakes fish, soil, and human adipose tissue. Because of the potential for human exposure, the present study was designed to investigate the short-term effects of photomirex on the in vitro perfused ovary of the rat. Adult Sprague-Dawley rat ovaries were isolated and perfused for a total of 6 h with Medium 199. Following a 2-h baseline period, 10(-4) M of photomirex was administered to the medium. Control ovaries received medium or DMSO (vehicle control). Significant effects of perfusion and chemical intervention were identified using lactate dehydrogenase enzyme, glucose utilization, lactate, pyruvate, and flow:pressure ratio as markers of toxicity (P < 0.05). Lactate:pyruvate ratio, glutathione, and oxygen consumption did not demonstrate significant effects. Post hoc tests showed that there were significant differences between the DMSO + photomirex group and the control group (M199) using lactate dehydrogenase as a marker of toxicity. Pyruvate concentration was also reduced significantly after perfusion with DMSO + photomirex compared to M199 only and DMSO only (P < 0.05). Histopathologic changes were not discernible by light microscopy. These results suggest that metabolic and respiratory processes of the ovary are acutely sensitive to perturbation with photomirex in the in vitro perfused rat ovary model.
Todoroff
E C
EC
Department of Obstetrics and Gynaecology, University of Calgary, Alberta, Canada.
Sevcik
M
M
Villeneuve
D C
DC
Foster
W G
WG
Jarrell
J F
JF
D000818:Animals; D001835:Body Weight / Q000187:drug effects; D004032:Diet; D005260:Female; D005947:Glucose / Q000378:metabolism; D005978:Glutathione / Q000378:metabolism; D066298:In Vitro Techniques; D019344:Lactic Acid / Q000378:metabolism; D008917:Mirex / Q000031:analogs & derivatives* / Q000493:pharmacokinetics / Q000633:toxicity; D009929:Organ Size / Q000187:drug effects; D010053:Ovary / Q000187:drug effects*; D010477:Perfusion; D019289:Pyruvic Acid / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D012989:Soil Pollutants / Q000493:pharmacokinetics / Q000633:toxicity*; D014018:Tissue Distribution; D014874:Water Pollutants, Chemical / Q000493:pharmacokinetics / Q000633:toxicity*
D012989:Soil Pollutants; D014874:Water Pollutants, Chemical; D019344:Lactic Acid; C016665:photomirex; D019289:Pyruvic Acid; D005978:Glutathione; D005947:Glucose; D008917:Mirex