Regulation of placental monocyte chemotactic and activating factor during pregnancy and chorioamnionitis.
A number of placental cytokines participate in the feto-maternal defence mechanism. Monocyte chemotactic and activating factor (MCAF) is one of these chemokines. We investigated the pattern of placental MCAF production, the localization of MCAF-producing cells in the placenta, and alterations in its expression in chorioamnionitis. The amounts of MCAF protein produced by the placenta increased during pregnancy irrespective of the presence of uterine contraction. MCAF mRNA was expressed at equivalent levels in the first and second trimester placenta, but at higher levels in the third trimester placenta. Chorioamnionitis in the third trimester placenta induced a 10-fold increase in MCAF protein production and a 3-fold increase in MCAF mRNA level. Immunohistochemical analysis of the placenta revealed the MCAF-producing cells to be trophoblasts. Stimulation of placental cells with lipopolysaccharide augmented MCAF production. These results indicate unique transcriptional and developmental regulation of MCAF mRNA and protein production during pregnancy and chorioamnionitis. Placental MCAF may be involved in the feto-maternal defence mechanism by activating feto-placental and maternal monocytes in chorioamnionitis.
Shimoya
K
K
Department of Obstetrics and Gynecology, Osaka University Medical School, Suita, Japan.
Matsuzaki
N
N
Sawai
K
K
Saji
F
F
Murata
Y
Y
Yasumoto
K
K
Su
S
S
Mukaida
N
N
Matsushima
K
K
D015153:Blotting, Western; D018932:Chemokine CCL2 / Q000032:analysis / Q000096:biosynthesis* / Q000378:metabolism*; D002821:Chorioamnionitis / Q000378:metabolism* / Q000473:pathology; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D010920:Placenta / Q000737:chemistry / Q000166:cytology / Q000378:metabolism*; D011247:Pregnancy
D018932:Chemokine CCL2