Adaptation of the cytokinesis-block technique for genotoxicity assay on L929 cell line.
The in vitro micronucleus assay was used as a method for evaluation of chromosomal damage in L929 cells. Known genotoxins were used to induce micronuclei: bleomycin the direct acting agent, and cyclophosphamide the substance which requires metabolic activation. The cytokinesis-block technique and the use of exogenous metabolizing system is precisely described.
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Drug Institute, Warsaw, Poland.
D000818:Animals; D001761:Bleomycin / Q000633:toxicity; D002460:Cell Line; D003520:Cyclophosphamide / Q000633:toxicity; D005347:Fibroblasts / Q000187:drug effects; D051379:Mice; D015162:Micronucleus Tests / Q000379:methods*; D009153:Mutagens / Q000633:toxicity*
D009153:Mutagens; D001761:Bleomycin; D003520:Cyclophosphamide