title: Immunolocalization of myotonic dystrophy protein kinase in corbular and junctional sarcoplasmic reticulum of human cardiac muscle.
abstract: The subcellular localization of myotonic dystrophy protein kinase has been examined in human cardiac muscles with confocal laser-scanning microscopy and electron microscopy. A polyclonal antibody was produced against the synthesized peptide from a human kinase cDNA clone. We checked the antibody specificity for cardiac myotonic dystrophy protein kinase using an immunoblotting technique. Immunoblotting of extract from human cardiac muscles showed mainly 70 kDa and 55 kDa molecular weight bands. Confocal images of the protein kinase immunostaining showed striated banding patterns similar to those of skeletal muscles. In addition, the kinase was strongly detected around the intercalated disc. Immunoelectron microscopy showed that the kinase was mainly expressed in both corbular and junctional sarcoplasmic reticulum, but not in network sarcoplasmic reticulum. These results suggest that myotonic dystrophy protein kinase may be involved in the modulation of Ca2+ homeostasis in cardiac myofibres.
authors.affiliation: Department of Anatomy, Yamanashi Medical University, Tokyo, Japan.
authors.forename: H
authors.initials: H
authors.lastname: Ueda
authors.forename: N
authors.initials: N
authors.lastname: Kameda
authors.forename: T
authors.initials: T
authors.lastname: Baba
authors.forename: N
authors.initials: N
authors.lastname: Terada
authors.forename: M
authors.initials: M
authors.lastname: Shimokawa
authors.forename: M
authors.initials: M
authors.lastname: Yamamoto
authors.forename: S
authors.initials: S
authors.lastname: Ishiura
authors.forename: T
authors.initials: T
authors.lastname: Kobayashi
authors.forename: S
authors.initials: S
authors.lastname: Ohno
mesh_terms: D015153:Blotting, Western; D006801:Humans; D018613:Microscopy, Confocal; D008856:Microscopy, Fluorescence; D016253:Microscopy, Immunoelectron; D009206:Myocardium / Q000201:enzymology* / Q000648:ultrastructure; D065636:Myotonin-Protein Kinase; D017346:Protein Serine-Threonine Kinases / Q000032:analysis*; D012519:Sarcoplasmic Reticulum / Q000201:enzymology* / Q000648:ultrastructure
chemical_list: C585517:DMPK protein, human; D065636:Myotonin-Protein Kinase; D017346:Protein Serine-Threonine Kinases