Rapid increase of NGF, BDNF and NT-3 mRNAs in inflamed bladder.
Nerve growth factor (NGF) is a mediator of hyperalgesia and has been previously associated with sensory and reflex changes after inflammation of the urinary bladder. A sensitive assay was developed to examine neurotrophin gene expression after bladder inflammation by turpentine, which causes a short-lived inflammatory response. Two hours, but not 6 or 24 h after induction of inflammation, there were significant increases in levels of NGF, brain-derived neurotrophic factor and neurotrophin-3 mRNAs. NGF immunoreactivity was elevated with a similar time course to its mRNA. Our results suggest that during bladder inflammation, endogenous NGF is rapidly up-regulated and released to mediating sensory and reflex changes. Brain-derived neurotrophic factor and neurotrophin-3 may also have a role in the inflammatory response.
Oddiah
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D
Division of Biochemistry & Molecular Biology, UMDS, Guy's Hospital, London, UK.
Anand
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P
McMahon
S B
SB
Rattray
M
M
D000818:Animals; D019208:Brain-Derived Neurotrophic Factor / Q000096:biosynthesis*; D017931:DNA Primers; D005260:Female; D017403:In Situ Hybridization; D007249:Inflammation / Q000139:chemically induced / Q000378:metabolism; D009414:Nerve Growth Factors / Q000096:biosynthesis*; D020933:Neurotrophin 3; D012333:RNA, Messenger / Q000096:biosynthesis*; D051381:Rats; D017208:Rats, Wistar; D014158:Transcription, Genetic; D014425:Turpentine; D001743:Urinary Bladder / Q000378:metabolism* / Q000503:physiopathology
D019208:Brain-Derived Neurotrophic Factor; D017931:DNA Primers; D009414:Nerve Growth Factors; D020933:Neurotrophin 3; D012333:RNA, Messenger; D014425:Turpentine