title: Changes in E. coli inorganic pyrophosphatase structure induced by binding of metal activators.
abstract: The three-dimensional structures of E. coli inorganic pyrophosphatase (PPase) and its complexes with Mn2+ in a high affinity site and with Mg2+ in high and low affinity sites determined by authors in 1994-1996 at 1.9-2.2 A resolution are compared. Metal ion binding initiates the shifts of alpha-carbon atoms and of functional groups and rearrangement of non-covalent interaction system of hexameric enzyme molecule. As a result, the apoPPase with six equal subunits turns after Mg2+ binding into the structure with three types of subunits distinguished by structure and occupance of the low affinity Mg2+ site. Induced asymmetry reflects the subunit interactions and cooperativity between Mg2+ binding sites. These molecular rearrangements are structural basis to account for special features of the enzyme behavior and to propose one of the pathways for enzymatic activity regulation of constitutive PPases in vivo.
authors.affiliation: Belozersky Institute of Physico-Chemical Biology, Lomonosov Moscow State University, Moscow, 119899, Russia. avaeva@protchem.genebee. msu.su
authors.forename: S M
authors.initials: SM
authors.lastname: Avaeva
authors.forename: E V
authors.initials: EV
authors.lastname: Rodina
authors.forename: N N
authors.initials: NN
authors.lastname: Vorobyeva
authors.forename: S A
authors.initials: SA
authors.lastname: Kurilova
authors.forename: T I
authors.initials: TI
authors.lastname: Nazarova
authors.forename: V A
authors.initials: VA
authors.lastname: Sklyankina
authors.forename: V Y
authors.initials: VY
authors.lastname: Oganessyan
authors.forename: E H
authors.initials: EH
authors.lastname: Harutyunyan
mesh_terms: D001051:Apoenzymes / Q000737:chemistry / Q000378:metabolism; D002118:Calcium / Q000378:metabolism; D020134:Catalytic Domain; D018360:Crystallography, X-Ray; D004926:Escherichia coli / Q000201:enzymology*; D043564:Inorganic Pyrophosphatase; D008274:Magnesium / Q000378:metabolism; D008345:Manganese / Q000378:metabolism; D008958:Models, Molecular; D011487:Protein Conformation; D011755:Pyrophosphatases / Q000737:chemistry* / Q000378:metabolism*; D011994:Recombinant Proteins / Q000737:chemistry / Q000378:metabolism
chemical_list: D001051:Apoenzymes; D011994:Recombinant Proteins; D008345:Manganese; D011755:Pyrophosphatases; D043564:Inorganic Pyrophosphatase; D008274:Magnesium; D002118:Calcium