Binding of paratropomyosin to beta-connectin from chicken skeletal muscle.
We found that paratropomyosin bound to beta-connectin, in examining binding of paratropomyosin at the junction of A- and I-bands of sarcomeres. The turbidity of a mixture of beta-connectin and paratropomyosin was greater with more paratropomyosin added, but high concentrations of Ca2+ suppress this increase. These results suggest that paratropomyosin is released from connectin filaments at the A-I junction region by increased concentrations of calcium ions in postmortem skeletal muscles.
Yamanoue
M
M
Department of Biofunctional Chemistry, Faculty of Agriculture, Kobe University, Japan.
Fei
S
S
Okayama
T
T
D000818:Animals; D002122:Calcium Chloride / Q000378:metabolism; D002645:Chickens; D064211:Connectin; D004591:Electrophoresis, Polyacrylamide Gel; D008565:Membrane Proteins / Q000378:metabolism*; D009124:Muscle Proteins / Q000378:metabolism*; D018482:Muscle, Skeletal / Q000378:metabolism*; D011180:Postmortem Changes; D011485:Protein Binding; D011494:Protein Kinases / Q000378:metabolism*; D014335:Tropomyosin / Q000378:metabolism*
D064211:Connectin; D008565:Membrane Proteins; D009124:Muscle Proteins; D014335:Tropomyosin; D011494:Protein Kinases; D002122:Calcium Chloride