title: KID-1, a protein kinase induced by depolarization in brain.
abstract: Membrane depolarization leads to changes in gene expression that modulate neuronal plasticity. Using representational difference analysis, we have identified a previously undiscovered cDNA, KID-1 (kinase induced by depolarization), that is induced by membrane depolarization or forskolin, but not by neurotrophins or growth factors, in PC12 pheochromocytoma cells. KID-1 is an immediate early gene that shares a high degree of sequence similarity with the family of PIM-1 serine/threonine protein kinases. Recombinant KID-1 fusion protein is able to catalyze both histone phosphorylation and autophosphorylation. KID-1 mRNA is present in a number of unstimulated tissues, including brain. In response to kainic acid and electroconvulsive shock-induced seizures, KID-1 is induced in specific regions of the hippocampus and cortex.
authors.affiliation: Department of Pediatrics, UCLA Center for the Health Sciences, Los Angeles, California 90095-1570, USA.
authors.forename: J D
authors.initials: JD
authors.lastname: Feldman
authors.forename: L
authors.initials: L
authors.lastname: Vician
authors.forename: M
authors.initials: M
authors.lastname: Crispino
authors.forename: G
authors.initials: G
authors.lastname: Tocco
authors.forename: V L
authors.initials: VL
authors.lastname: Marcheselli
authors.forename: N G
authors.initials: NG
authors.lastname: Bazan
authors.forename: M
authors.initials: M
authors.lastname: Baudry
authors.forename: H R
authors.initials: HR
authors.lastname: Herschman
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D001921:Brain / Q000201:enzymology*; D002384:Catalysis; D005576:Colforsin / Q000494:pharmacology; D004790:Enzyme Induction; D005786:Gene Expression Regulation; D017781:Genes, Immediate-Early; D006133:Growth Substances / Q000494:pharmacology; D006657:Histones / Q000378:metabolism; D008564:Membrane Potentials / Q000502:physiology; D008969:Molecular Sequence Data; D009473:Neuronal Plasticity / Q000502:physiology; D016716:PC12 Cells; D010766:Phosphorylation; D017346:Protein Serine-Threonine Kinases / Q000096:biosynthesis* / Q000737:chemistry / Q000235:genetics; D011518:Proto-Oncogene Proteins / Q000737:chemistry; D051573:Proto-Oncogene Proteins c-pim-1; D051381:Rats; D011993:Recombinant Fusion Proteins / Q000378:metabolism
chemical_list: D006133:Growth Substances; D006657:Histones; D011518:Proto-Oncogene Proteins; D011993:Recombinant Fusion Proteins; D005576:Colforsin; C496637:Pim1 protein, rat; C113398:Pim3 protein, rat; D017346:Protein Serine-Threonine Kinases; D051573:Proto-Oncogene Proteins c-pim-1