title: Primary epitopes of chicken egg yolk antibodies to peptidophosphogalactomannan.
abstract: Egg yolks from hens immunized with peptidophosphogalactomannan (pPGalMan(ii)), which contains 10 phosphocholine diester residues and is secreted by Penicillium fellutanum, contain antibodies against 5-O-beta-D-galactofuranosyl epitopes. These epitopes were the only significant determinants in pPGalMan(ii). Approximately 60-fold less pPGalMan(ii) (1.6 microM galactofuran chains) was required for 50% inhibition than galactofurano-oligosaccharides or pPGalMan containing two galactofuranosyl residues per chain.
authors.affiliation: Department of Microbiology and Cell Science, Institute of Food and Agricultural Sciences, University of Florida, Gainesville 32611-0700, USA.
authors.forename: B A
authors.initials: BA
authors.lastname: Tuekam
authors.forename: S J
authors.initials: SJ
authors.lastname: Bonetti
authors.forename: J E
authors.initials: JE
authors.lastname: Gander
mesh_terms: D000818:Animals; D000908:Antibodies, Fungal / Q000276:immunology* / Q000302:isolation & purification; D000937:Antigen-Antibody Reactions; D002240:Carbohydrate Sequence; D002645:Chickens / Q000276:immunology*; D004530:Egg Yolk / Q000276:immunology*; D000939:Epitopes; D005690:Galactose / Q000031:analogs & derivatives; D006025:Glycosaminoglycans / Q000737:chemistry / Q000276:immunology*; D008351:Mannans; D008969:Molecular Sequence Data; D010407:Penicillium / Q000276:immunology
chemical_list: D000908:Antibodies, Fungal; D000939:Epitopes; D006025:Glycosaminoglycans; D008351:Mannans; C012990:galactomannan; D005690:Galactose