Molecular cloning of a light-inducible gene (lipA) encoding a novel pilin from Arthrobacter photogonimos.
Development of pili on cells of Arthrobacter photogonimos is induced by photo-oxidative conditions. The nucleotide sequence was determined of a light-inducible gene (lipA) that encodes the precursor of a light-inducible pilin (designated LIP), a polypeptide of 212 amino acids. The N-terminal leader peptide includes a typical signal sequence with a consensus cleavage site for signal peptidase 1 after residue 28, which should generate N-terminal arginine. However, the next amino acid, alanine, is the N-terminal residue of the mature protein. The abundance of charged amino acids (27% of total), a calculated pI of 9.98, and recovery of mostly monomers when cells were washed with 1 M NaCl suggest that electrostatic interactions play a dominant role in association of LIP, a novel mechanism for assembly of pili.
Yang
H S
HS
Graduate Program in Molecular and Cellular Biology, Arizona State University, Tempe 85287-1601, USA.
Hoober
J K
JK
D000595:Amino Acid Sequence; D000596:Amino Acids / Q000032:analysis; D001173:Arthrobacter / Q000235:genetics*; D001426:Bacterial Proteins / Q000737:chemistry / Q000235:genetics*; D001483:Base Sequence; D003001:Cloning, Molecular; D004926:Escherichia coli / Q000235:genetics; D015964:Gene Expression Regulation, Bacterial / Q000528:radiation effects*; D005798:Genes, Bacterial / Q000235:genetics; D008027:Light; D008969:Molecular Sequence Data; D011401:Promoter Regions, Genetic / Q000235:genetics; D011498:Protein Precursors
D000596:Amino Acids; D001426:Bacterial Proteins; D011498:Protein Precursors; C112932:lipA pre-pilin protein, Arthrobacter photogonimos