Determination of host specificity of cowpea miscellany Rhizobium spp. by nodABC-lacZ fusion.
Cowpea miscellany group of rhizobia are generally broad host range. Transconjugants of these cowpea rhizobial isolates having nodABC-lacZ fusion were monitored for flavonoid/root exudate-induced activation of the nod genes in terms of beta-galactosidase activity, thus determining the potential host range of the rhizobial isolates.
Pandya
S
S
Department of Microbiology & Biotechnology Centre, Faculty of Science, M.S. University of Baroda, India.
Uchil
P
P
Subramanian
M
M
Desai
A
A
D000217:Acyltransferases / Q000235:genetics*; D000581:Amidohydrolases / Q000235:genetics*; D019677:Artificial Gene Fusion; D001426:Bacterial Proteins / Q000235:genetics*; D007763:Lac Operon; D017351:N-Acetylglucosaminyltransferases / Q000235:genetics*; D018532:Pisum sativum / Q000382:microbiology*; D012231:Rhizobium / Q000235:genetics*
D001426:Bacterial Proteins; C073606:nodB protein, Bacteria; D000217:Acyltransferases; C060770:NodA protein, Rhizobiales; D017351:N-Acetylglucosaminyltransferases; C060771:NodC protein, Rhizobiales; D000581:Amidohydrolases