Enhanced gene transfer with fusogenic liposomes containing vesicular stomatitis virus G glycoprotein.
Exposure of Lipofectin-DNA complexes to the partially purified G glycoprotein of the vesicular stomatitis virus envelope (VSV-G) results in loss of serum-mediated inhibition and in enhanced efficiency of gene transfer. Sucrose density gradient sedimentation analysis indicated that the VSV-G associates physically with the DNA-lipid complex to produce a VSV-G liposome. The ability to incorporate surrogate viral or cellular envelope components such as VSV-G into liposomes may allow more-efficient and possibly targeted gene delivery by lipofection, both in vitro and in vivo.
Abe
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A
Department of Pediatrics, Center for Molecular Genetics, University of California, San Diego, La Jolla, California 92093-0634, USA.
Miyanohara
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A
Friedmann
T
T
D003470:Culture Media; D004279:DNA, Viral / Q000008:administration & dosage; D018014:Gene Transfer Techniques; D008081:Liposomes; D008562:Membrane Glycoproteins; D010714:Phosphatidylethanolamines / Q000008:administration & dosage*; D014162:Transfection; D014759:Viral Envelope Proteins / Q000008:administration & dosage* / Q000302:isolation & purification
D003470:Culture Media; D004279:DNA, Viral; C028391:G protein, vesicular stomatitis virus; D008081:Liposomes; D008562:Membrane Glycoproteins; D010714:Phosphatidylethanolamines; D014759:Viral Envelope Proteins; C020888:1,2-dielaidoylphosphatidylethanolamine