A quick method to extract DNA from paraffin-embedded tissues.
BACKGROUND
The amplification power of the polymerase chain reaction (PCR) technique has had great impact in molecular analysis, and DNA extraction is a common requirement in retrospective studies utilizing PCR as a tool. Conventional methods used in deparaffinization, harvesting and purification of DNA from paraffin-embedded tissue are time consuming and cause a significant loss in the yield of DNA.


METHODS
We utilized heating-melting-cooling removal of paraffin, digestion of the sample with proteinase K and purification of the extracted DNA by a microconcentrator. The products, after PCR amplification of the p53 gene exon 8, were used to make a comparison between our method and the conventional xylene-phenol-choloform method.


RESULTS
The amplified products from our method were superior to that of the conventional method.


CONCLUSIONS
The method we propose has a better recovery of DNA and is more time efficient.
Tseng
C K
CK
Department of Radiation Oncology, Chang Gung Memorial Hospital, Taoyuan, Taiwan, R.O.C.
Tsang
N M
NM
Kao
S C
SC
Chen
S Y
SY
Chen
Y P
YP
D004247:DNA / Q000302:isolation & purification*; D006801:Humans; D010232:Paraffin; D016133:Polymerase Chain Reaction; D016610:Tissue Embedding
D010232:Paraffin; D004247:DNA