Localization of glutamate receptors in dorsal horn of rat spinal cord.
Immunocytochemical localization of metabotropic glutamate receptors (mGluRs) and ionotropic glutamate receptors (NMDA-type: NMDAR1 and NMDAR2A-C; AMPA-type: GluR1-4) was performed on sections of rat dorsal horn. Immunoreactivity for mGluR1 alpha was detected in laminae I-III of the dorsal horn, whilst mGluR2/3 immunoreactivity was detected primarily in lamina III. Immunoreactivity for NMDAR1, GluR1, GluR2, GluR2/3, GluR4 and GluR5/6/7 was strongly localized in neuronal elements of laminae I-III. Immunoreactivity for NMDAR2B was localized in laminae I-III. No mGluR5, NMDAR2A and NMDAR2C immunoreactivity was detected. In addition, immunoreactivity for receptors was found to co-localize with immunoreactivity for glutamate in the dorsal horn. The present results indicate that glutamate receptors are differentially localized in neuronal elements of dorsal horn where receptor-neurotransmitter interaction takes place.
Yung
K K
KK
Department of Biology, Hong Kong Baptist University, Kowloon Tong, China.
D000818:Animals; D005260:Female; D007150:Immunohistochemistry; D009474:Neurons / Q000166:cytology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D017470:Receptors, Glutamate / Q000032:analysis* / Q000145:classification; D016194:Receptors, N-Methyl-D-Aspartate / Q000032:analysis* / Q000145:classification; D013116:Spinal Cord / Q000166:cytology*
D017470:Receptors, Glutamate; D016194:Receptors, N-Methyl-D-Aspartate