title: Using specific polyclonal antibodies to study the malolactic enzyme from Leuconostoc oenos and other lactic acid bacteria.
abstract: Specific polyclonal antibodies directed against the malolactic enzyme of Leuconostoc oenos were obtained. Despite the homologies between the malolactic enzymes from Leuc. oenos and Lactococcus lactis, no immunological relationship was detected with the L. lactis malolactic enzyme, suggesting differences in their structural organization. The use of the antiserum also demonstrated that the problem of heterologous expression occurring in the recombinant Escherichia coli strain (Labarre et al. 1996a) resulted in a low synthesis of the malolactic enzyme from Leuc. oenos. Moreover, a small amount of the protein was found to be peripherally associated to the membrane of Leuc. oenos.
authors.affiliation: Laboratory of Microbiology, ENSBANA, Dijon, France.
authors.forename: C
authors.initials: C
authors.lastname: Labarre
authors.forename: J F
authors.initials: JF
authors.lastname: Cavin
authors.forename: C
authors.initials: C
authors.lastname: Diviès
authors.forename: J
authors.initials: J
authors.lastname: Guzzo
mesh_terms: D000918:Antibody Specificity; D001426:Bacterial Proteins / Q000276:immunology* / Q000378:metabolism; D015153:Blotting, Western; D004591:Electrophoresis, Polyacrylamide Gel; D007934:Leuconostoc / Q000201:enzymology*; D008291:Malate Dehydrogenase / Q000276:immunology* / Q000378:metabolism
chemical_list: D001426:Bacterial Proteins; C037731:malolactic enzyme; D008291:Malate Dehydrogenase