[Induction of apoptosis through NGF/p75NTR in human glioma cells treated with propentofylline].
Propentofylline (PPF) is a xanthine derivative and it has been reported that PPF stimulated the synthesis/secretion of nerve growth factor (NGF) by cultured astroglial cells. We have reported that PPF induced apoptosis in human glioma cells and as its mechanism, up-regulation of NGF, Fas, Bax beta and down-regulation of Bcl-2 were assumed. Recent studies demonstrated the presence of apoptosis induced via NGF/p75NTR in developmental neuron, oligodendroglia. In the present study, to examine whether signal cascade via NGF/p75NTR participates the apoptosis in human glioma cells treated with PPF, we investigated the immunohistochemical study using anti-mouse monoclonal antibody, immunoprecipitation and northern blot analysis for p75NTR, NF-kappa B in human glioma cells (U251MG, T98G, U87) treated with PPF. We observed the up-regulation of p75NTR, translocation of NF-kappa B to the nucleus after treatment with PPF in these cell lines. According to above results, it is assumed that apoptosis in human glioma cells treated with PPF was induced via NGF/p75NTR.
Yamaguchi
H
H
Department of Neurosurgery, Hokuto Hospital, Hokkaido, Japan.
Ukita
H
H
Murakami
H
H
Chiba
S
S
Oshima
Y
Y
Ogata
A
A
Ikeda
M
M
Matsumoto
A
A
Satone
A
A
Ide
W
W
Hashimoto
I
I
Kamada
H
H
D017209:Apoptosis / Q000187:drug effects*; D001932:Brain Neoplasms / Q000473:pathology*; D002352:Carrier Proteins / Q000502:physiology*; D005910:Glioma / Q000473:pathology*; D006801:Humans; D009414:Nerve Growth Factors / Q000502:physiology*; D009419:Nerve Tissue Proteins / Q000502:physiology*; D017978:Receptors, Growth Factor; D017475:Receptors, Nerve Growth Factor; D014407:Tumor Cells, Cultured; D014970:Xanthines / Q000494:pharmacology*
D002352:Carrier Proteins; C484065:NGFR protein, human; D009414:Nerve Growth Factors; D009419:Nerve Tissue Proteins; D017978:Receptors, Growth Factor; D017475:Receptors, Nerve Growth Factor; D014970:Xanthines; C032114:propentofylline