Heterogeneous pattern of gene expression in cloned cell lines established from a rat transplantable osteosarcoma lung metastatic nodule.
We have established three cloned cell lines (COS1NR, COS2NR and COS4NR) from the lung metastatic nodule of a highly metastatic variant of rat transplantable osteosarcoma, C-SLM. All three clones shared the same morphological characteristics and tumorigenicity, but their growth rates in vitro and metastatic ability in vivo differed from each other. Single-strand conformation polymorphism (SSCP) analysis revealed all three clones to have the same p53 gene mutation and parent C-SLM tumor. On the other hand, Northern blot analysis showed a different pattern of expression for the genes, c-fos, c-jun, c-Ha-ras, transin (rat stromelysin), bone Gla protein (osteocalsin) and nm23/NDP kinase. These results indicate the presence of a heterogeneous cell population in terms of the different pattern of gene expression in a lung metastatic nodule of rat osteosarcoma and the present newly established cell lines will be useful for further investigation of the biological behavior of osteosarcomas.
Honoki
K
K
Department of Orthopedic Surgery, Nara Medical University, Kashihara, Japan.
Mori
T
T
Tsutsumi
M
M
Tsujiuchi
T
T
Kido
A
A
Morishita
T
T
Miyauchi
Y
Y
Dohi
Y
Y
Mii
Y
Y
Tamai
S
S
Konishi
Y
Y
D000818:Animals; D002999:Clone Cells; D015870:Gene Expression; D016158:Genes, p53; D008175:Lung Neoplasms / Q000235:genetics* / Q000556:secondary*; D008297:Male; D009154:Mutation; D009368:Neoplasm Transplantation; D012516:Osteosarcoma / Q000473:pathology*; D018807:Polymorphism, Single-Stranded Conformational; D051381:Rats; D011916:Rats, Inbred F344