title: Coreceptor usage of human immunodeficiency virus type 2 primary isolates and biological clones is broad and does not correlate with their syncytium-inducing capacities.
abstract: Entry of human immunodeficiency virus type 1 (HIV-1) into target cells is mediated by binding of the surface envelope glycoprotein to the CD4 molecule. Interaction of the resulting CD4-glycoprotein complex with alpha- or beta-chemokine receptors, depending on the biological phenotype of the virus, then initiates the fusion process. Here, we show that primary HIV-2 isolates and biological clones, in contrast to those of HIV-1, may use a broad range of coreceptors, including CCR-1, CCR-3, CCR-5, and CXCR-4. The syncytium-inducing capacity of these viruses did not correlate with the ability to infect via CXCR-4 or any other coreceptor. One cell-free passage of the intermediate isolates in mitogen-stimulated, CD8+ cell-depleted peripheral blood mononuclear cells resulted in the outgrowth of variants with CCR-5 only, whereas the coreceptor usage of late and early isolates did not change. Since HIV-2 is less pathogenic in vivo than HIV-1, these data suggest that HIV pathogenicity in vivo is not directly related to the spectrum of coreceptors used in in vitro systems.
authors.affiliation: Institute of Virology, Erasmus University Rotterdam, Rotterdam, The Netherlands.
authors.forename: C
authors.initials: C
authors.lastname: Guillon
authors.forename: M E
authors.initials: ME
authors.lastname: van der Ende
authors.forename: P H
authors.initials: PH
authors.lastname: Boers
authors.forename: R A
authors.initials: RA
authors.lastname: Gruters
authors.forename: M
authors.initials: M
authors.lastname: Schutten
authors.forename: A D
authors.initials: AD
authors.lastname: Osterhaus
mesh_terms: D015498:HIV-2 / Q000502:physiology*; D006801:Humans; D019713:Receptors, CCR5 / Q000502:physiology*; D019718:Receptors, CXCR4 / Q000502:physiology*; D015728:Receptors, HIV / Q000502:physiology*
chemical_list: D019713:Receptors, CCR5; D019718:Receptors, CXCR4; D015728:Receptors, HIV