Regulation of phospholipase C-delta by GTP-binding proteins-rhoA as an inhibitory modulator.
The regulation of Phospholipase C (PLC)delta activity remains obscure. These studies show that PLCdelta1 activity is significantly enhanced by both guanosine thiotriphosphate (GTPgammaS) and Clostridium botulinum exoenzyme C3 (C3) but not by aluminium fluoride. C3 ADP ribosylated a 21-kDa protein in the PLCdelta1 preparation and Western blotting identified rhoA in these samples. RhoA acts as an inhibitory modulator of PLCdelta activity.
Hodson
E A
EA
Physiology Laboratory, University of Oxford, Parks Road, Oxford OX1 3PT, UK.
Ashley
C C
CC
Hughes
A D
AD
Lymn
J S
JS
D036002:ADP Ribose Transferases / Q000494:pharmacology; D000246:Adenosine Diphosphate Ribose / Q000378:metabolism; D000255:Adenosine Triphosphate / Q000031:analogs & derivatives / Q000494:pharmacology; D000818:Animals; D000931:Antidotes / Q000494:pharmacology; D001905:Botulinum Toxins; D002417:Cattle; D005591:Chemical Fractionation; D003676:Deferoxamine / Q000494:pharmacology; D004789:Enzyme Activation / Q000187:drug effects; D020558:GTP Phosphohydrolases / Q000187:drug effects / Q000378:metabolism; D019204:GTP-Binding Proteins / Q000037:antagonists & inhibitors* / Q000737:chemistry / Q000378:metabolism / Q000494:pharmacology*; D016244:Guanosine 5'-O-(3-Thiotriphosphate) / Q000737:chemistry / Q000494:pharmacology; D007527:Isoenzymes / Q000737:chemistry / Q000187:drug effects* / Q000302:isolation & purification; D054803:Phospholipase C delta; D012969:Sodium Fluoride / Q000494:pharmacology; D013462:Sulfur Radioisotopes; D010738:Type C Phospholipases / Q000737:chemistry / Q000187:drug effects* / Q000302:isolation & purification; D020742:rhoA GTP-Binding Protein
D000931:Antidotes; D007527:Isoenzymes; D013462:Sulfur Radioisotopes; D000246:Adenosine Diphosphate Ribose; D016244:Guanosine 5'-O-(3-Thiotriphosphate); D000255:Adenosine Triphosphate; D012969:Sodium Fluoride; D036002:ADP Ribose Transferases; C063007:exoenzyme C3, Clostridium botulinum; D010738:Type C Phospholipases; D054803:Phospholipase C delta; D001905:Botulinum Toxins; D020558:GTP Phosphohydrolases; D019204:GTP-Binding Proteins; D020742:rhoA GTP-Binding Protein; D003676:Deferoxamine