title: Selective NMR Experiments on Macromolecules: Implementation and Analysis of QUIET-NOESY.
abstract: The QUIET-NOESY experiment (Zwahlen et al., J. Am. Chem Soc. 116, 362-368, 1994) is applied to measure the mobility of the flexible extensions in the large aggregate (800 kDa) of a small heat-shock protein. The proper choices of the experimental protocol and parameters are discussed in order to employ a simplified data analysis procedure. Further experimental verification of the proposed strategy is also presented using the cyclic peptide gramicidin S as a model compound. Under suitable conditions, the determinations based on the analysis of QUIET-NOESY data are affected to a negligible extent by the approximations that are introduced by the proposed approach. Copyright 1998 Academic Press.
authors.affiliation: Dipartimento di Scienze e Tecnologie Biomediche, Università degli Studi di Udine, Via Gervasutta, 48, Udine, 33100, Italy
authors.initials: G
authors.lastname: Esposito
authors.initials: P
authors.lastname: Viglino
authors.initials: F
authors.lastname: Fogolari
authors.initials: M
authors.lastname: Gaestel
authors.initials: JA
authors.lastname: Carver