title: Microheterogeneity and AMP-FLP analysis of the 3' flanking interleukin-6 VNTR polymorphism in central Spain.
abstract: The 3' flanking region of the interleukin-6 gene is polymorphic due to the existence of a hyper-variable region consisting of a number of A + T rich variable repeated DNA sequences (VNTR). We used specific primers to amplify this particular VNTR system by PCR in 222 unrelated normal Spaniards from Madrid, Spain. A model of inheritance comprising of five different allele classes was proposed and frequencies evaluated as follows: B4, 0.635; B3.1, 0.029; B3, 0.270; B2, 0.038; B1, 0.027. Also, examples of inheritance of mendelian microheterogeneity are shown. Heterozigosity index was calculated (H = 0.5) and no departure from Hardy-Weinberg equilibrium was observed (chi 2 = 0.091, d. f. 1, p > 0.75).
authors.affiliation: Centro de Transfusión de Madrid, Spain.
authors.forename: E
authors.initials: E
authors.lastname: Arroyo
authors.forename: F
authors.initials: F
authors.lastname: Garcia-Sanchez
authors.forename: J M
authors.initials: JM
authors.lastname: Ruiz de la Cuesta
authors.forename: J L
authors.initials: JL
authors.lastname: Vicario
mesh_terms: D018740:Genetic Heterogeneity; D006801:Humans; D015850:Interleukin-6 / Q000235:genetics*; D018598:Minisatellite Repeats; D016133:Polymerase Chain Reaction / Q000379:methods; D011110:Polymorphism, Genetic; D013030:Spain
chemical_list: D015850:Interleukin-6