The effects of branched chain amino acid infusion on pain perception and plasma concentrations of monoamines.
Infusions of branched chain amino acids (BCAA) have been shown to have several CNS-mediated effects including antinociceptive action. We investigated the effects of BCAA infusion on pain perception, respiratory control, and plasma monoamine concentrations. Six healthy female volunteers were given in a double-blind, random, crossover design an 8-h infusion (1.75 ml/kg/h) of either (a) Ringers lactate, (b) conventional 4% amino acid solution, or (c) 4% BCAA solution with intervals of at least 48 h. Respiratory control was evaluated with continuous capnography. Pain perception was measured using dental dolorimetry for sharp pain, and pain transmitted by afferent C-fibers was evaluated with tourniquet test. Changes in vigilance were measured using critical flicker fusion technique. Evaluations were made for baseline, and after 2.5, 5, and 8 h. Plasma samples were collected at the same time points for amino acid and monoamine analysis. BCAA infusion resulted in significant increases of plasma concentrations of all BCAAs, with a simultaneous decrease in concentrations of aromatic amino acids. Of the measured monoamines and their metabolites dihydroxyphenylacetic acid (DOPAC) decreased, showing significant treatment effect for BCAA. Despite these changes no significant effect of BCAAs on respiratory control, vigilance, or pain perception was observed. In conclusion, despite significant changes in plasma concentrations of both amino acids and DOPAC, BCAA infusion did not show any clinically relevant antinociceptive effect.
Kirvelä
O
O
Department of Anaesthesiology, Turku University Hospital, Finland.
Jaatinen
J
J
Scheinin
H
H
Kanto
J
J
D015102:3,4-Dihydroxyphenylacetic Acid / Q000097:blood; D000328:Adult; D000597:Amino Acids, Branched-Chain / Q000008:administration & dosage* / Q000097:blood; D000700:Analgesics / Q000008:administration & dosage; D015306:Biogenic Monoamines / Q000097:blood*; D004311:Double-Blind Method; D004837:Epinephrine / Q000097:blood; D005260:Female; D006801:Humans; D007262:Infusions, Intravenous; D008734:Methoxyhydroxyphenylglycol / Q000031:analogs & derivatives / Q000097:blood; D009638:Norepinephrine / Q000097:blood; D017288:Pain Threshold / Q000187:drug effects* / Q000502:physiology
D000597:Amino Acids, Branched-Chain; D000700:Analgesics; D015306:Biogenic Monoamines; D015102:3,4-Dihydroxyphenylacetic Acid; D008734:Methoxyhydroxyphenylglycol; C010117:3,4-dihydroxyphenylglycol; D009638:Norepinephrine; D004837:Epinephrine