Bioconversion of fumaric acid to succinic acid by recombinant E. coli.
Succinic acid was produced efficiently from fumaric acid by a recombinant E. coli strain DH5 alpha/pGC1002 containing multicopy fumarate reductase genes. The effects of initial fumaric acid and glucose concentration on the production of succinic acid were investigated. Succinic acid reached 41 to over 60 g/L in 48.5 h starting with 50 to 64 g/L fumaric acid. Significant substrate inhibition was observed at initial fumaric acid concentration of 90 g/L. L-Malic acid became the major fermentation product under these conditions. Provision of glucose (5-30 g/L) to the fermentation medium stimulated the initial succinic acid production rate over two folds.
Wang
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Laboratory of Renewable Resources Engineering, Purdue University, West Lafayette, IN 47907, USA.
Gong
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CS
Tsao
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GT
D019342:Acetic Acid / Q000737:chemistry; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism*; D005285:Fermentation; D005650:Fumarates / Q000378:metabolism*; D005947:Glucose / Q000737:chemistry; D008293:Malates / Q000737:chemistry; D013385:Succinate Dehydrogenase / Q000235:genetics; D019802:Succinic Acid / Q000378:metabolism*
D005650:Fumarates; D008293:Malates; C030298:malic acid; C032005:fumaric acid; D019802:Succinic Acid; D013385:Succinate Dehydrogenase; D005947:Glucose; D019342:Acetic Acid