Experimental toxoplasmosis, and immunosuppression (parasite evaluation and histological alterations)
With the purpose of studying immunosuppressant drug action on parasites, the effect of Cyclosporine and cortisone in experimental toxoplasma evaluation, a highly virulent strain of Toxoplasma gondii (RH strain) was used. The animals were divided in two groups: control and immunosuppressed. Two animals from each group were sacrificed daily. Evaluation was based on parasitemia and parasitism of peritoneal exsudate and presence of the parasite in heart, lung, liver, spleen, small bowel, brain, cerebellum, spinal cord and eyes. Parasite infestation in peritoneal exsudate was 10 times greater than parasitemia. Red spots were observed after the second day; after the fourth day, focal necrosis with softening and fluidification of brain tissue spattered throughout the nervous tissue with enhanced vascularization. Capillaries obstructed by Toxoplasmas gondii were found, causing infarction and necrosis due to toxic or enzymatic substances. Images suggestive of cells an "owl-eye", Cytomegalovirus-like were found, with T. gondii, both in the brain, as well as in the tear glands and small bowel. Blood stream parasite infestation was observed, no histopathological lesions were found in the remaining tissue, possibly due to lack of time, for all animals died within a week's observation. We conclude that immunosuppression has worsened the outcome, anticipating the disease in 24 hours.
Guimarães
A C
AC
Instituto Adolfo Lutz, Instituto de Medicina Tropical de São Paulo, Brazil.
Kawarabayashi
M
M
Nunes
E V
EV
Ferraz
S N
SN
Chiosini
C B
CB
Tolezano
J E
JE
Gama-Rodrigues
J J
JJ
Okumura
M
M
D000818:Animals; D000893:Anti-Inflammatory Agents / Q000494:pharmacology*; D016572:Cyclosporine / Q000494:pharmacology*; D006854:Hydrocortisone / Q000494:pharmacology*; D007165:Immunosuppression Therapy; D007166:Immunosuppressive Agents / Q000494:pharmacology*; D051379:Mice; D018512:Parasitemia; D014122:Toxoplasma / Q000187:drug effects*; D014124:Toxoplasmosis, Animal / Q000276:immunology*
D000893:Anti-Inflammatory Agents; D007166:Immunosuppressive Agents; D016572:Cyclosporine; D006854:Hydrocortisone