[Cell sensitivity to Fas-mediated apoptosis depends on three forms of Fas-antigen].
It is known that binding of specific anti-Fas antibodies to Fas-receptor induces apoptosis in various cell lines. But the mechanisms of functional regulation and realization of apoptosis remain so far unknown. We obtained HeLa cells transfected with cDNA of Fas-antigen, whose expression was located under the promoter controlled by isopropoyl beta-D-thiogalactopiranoside. Analysis of transfectants has shown that expression of Fas above a certain critical level leads to redistribution of Fas in the cells and is accompanied by changes in the cell cycles and cell sensitivity to the cytotoxic effects of anti-Fas and tumor necrosis factor (TNF). We propose that the sensitivity of cells to Fas-mediated apoptosis depends on the ratio of transmembrane, intracellular and soluble Fas-antigen in the cells.
Beletskaia
I V
IV
Institute of Biochemistry and Physiology of Microorganisms, Russian Academy of Sciences, Pushchino, Russia.
Nazarova
L F
LF
Nikonova
L V
LV
Abbasova
S G
SG
Beletskiĭ
I P
IP
D000906:Antibodies / Q000276:immunology; D017209:Apoptosis / Q000502:physiology*; D002453:Cell Cycle / Q000502:physiology; D018076:DNA, Complementary / Q000096:biosynthesis; D053222:Fas Ligand Protein; D006367:HeLa Cells; D006801:Humans; D007424:Intracellular Fluid / Q000378:metabolism; D007544:Isopropyl Thiogalactoside / Q000494:pharmacology; D008562:Membrane Glycoproteins / Q000378:metabolism; D012097:Repressor Proteins / Q000096:biosynthesis; D012995:Solubility; D014162:Transfection; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism; D019014:fas Receptor / Q000096:biosynthesis / Q000276:immunology / Q000502:physiology*
D000906:Antibodies; D018076:DNA, Complementary; C505661:FASLG protein, human; D053222:Fas Ligand Protein; D008562:Membrane Glycoproteins; D012097:Repressor Proteins; D014409:Tumor Necrosis Factor-alpha; D019014:fas Receptor; D007544:Isopropyl Thiogalactoside
Kletochnaia chuvstvitel'nost' k Fas-oposredovannomu apoptozu zavisit ot trekh form Fas-antigena.