title: Molecular cloning and characterization of starch-branching enzyme II from potato.
abstract: Full-length cDNA for starch branching enzyme (SBE) II of potato was isolated and sequenced. In potato, similarly to most other investigated plants, the SBE-II isoform differs from SBE-I by having an acidic amino-terminal extension and a shorter carboxyterminus. Two forms of SBE-II, migrating as 98 and 95 kDa proteins in 6% SDS-polyacrylamide gels, were associated to tuber starch. The latter form was 16 amino acids shorter in the amino terminus. Transcript of SBE-II was present in leaf tissue, whereas significant expression was not seen in tubers. On the other hand, a significant amount of SBE-I transcript was detected in tuber tissue but not in leaves.
authors.affiliation: Uppsala Genetic Center, Dept. of Cell Research, Swedish University of Agricultural Sciences.
authors.forename: C T
authors.initials: CT
authors.lastname: Larsson
authors.forename: J
authors.initials: J
authors.lastname: Khoshnoodi
authors.forename: B
authors.initials: B
authors.lastname: Ek
authors.forename: L
authors.initials: L
authors.lastname: Rask
authors.forename: H
authors.initials: H
authors.lastname: Larsson
mesh_terms: D015061:1,4-alpha-Glucan Branching Enzyme; D000595:Amino Acid Sequence; D015152:Blotting, Northern; D003001:Cloning, Molecular; D018076:DNA, Complementary; D005964:Glucosyltransferases / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D008969:Molecular Sequence Data; D017386:Sequence Homology, Amino Acid; D011198:Solanum tuberosum / Q000201:enzymology*; D013213:Starch
chemical_list: D018076:DNA, Complementary; D013213:Starch; D005964:Glucosyltransferases; D015061:1,4-alpha-Glucan Branching Enzyme; C112590:starch-branching enzyme II