Effects of O-glycosylation inhibitors on the differentiation of HL-60 cells.
The effects of O-glycosylation inhibitors on the growth and differentiation of the human acute promyeloblastic leukemia cell line HL-60 were studied to examine whether the O-glycosylation is needed for HL-60 cells to differentiate into granulocyte-like cells or monocyte-macrophage-like cells. N-Acetyl-alpha-D-galactosaminides, inhibitors of mucin-type oligosaccharide synthesis, and N-acetyl-beta-D-galactosaminides did not affect either growth or differentiation. beta-D-Xylosides, the artificial initiators of glycosaminoglycan synthesis, also were tested. Only 4-methylumbelliferyl-beta-D-xyloside induced HL-60 cells, to differentiate, and they differentiated into granulocyte-like cells, assessed by reduction of nitroblue tetrazolium, Giemsa staining, and esterase double-staining. The aglycon portion of 4-methylum-belliferyl-beta-D-xyloside, 4-methylumbelliferone, caused the differentiation. Thus we could find a new drug that induces the differentiation of HL-60 cells.
Shibuya
Y
Y
Department of Industrial Chemistry, School of Engineering, Tokai University, Kanagawa, Japan.
Sekiguchi
T
T
Suzuki
K
K
Takahashi
T
T
Nishikawa
Y
Y
D000964:Antimetabolites, Antineoplastic / Q000494:pharmacology*; D002454:Cell Differentiation / Q000187:drug effects; D006031:Glycosylation / Q000187:drug effects*; D006098:Granulocytes / Q000187:drug effects / Q000648:ultrastructure; D018922:HL-60 Cells; D006801:Humans; D006923:Hymecromone / Q000494:pharmacology
D000964:Antimetabolites, Antineoplastic; D006923:Hymecromone