title: Synthesis and cytotoxic activity of new peptides containing basic amino acid residues.
abstract: In search of more potent compounds endowed with a cytotoxic activity, a new series of basic peptides was synthesized using solid-phase methods. All peptides were purified by preparative reverse-phase HPLC and characterized by electrospray mass spectrometry. The cytotoxic activity was determined in cultured HeLa cells. The hexadecapeptides 5 and 6 showed a 50% inhibition at the concentration of 30 micrograms/ml. The salmina and the polyamino acids of L-arginine, L-histidine and L-lysine, containing sixteen residues, were virtually inactive. This demonstrates that a specific peptide sequence is necessary to obtain a positive response in HeLa test.
authors.affiliation: Dipartimento di Chimica Organica e Industriale, Milano, Italy.
authors.forename: F
authors.initials: F
authors.lastname: Chillemi
authors.forename: P
authors.initials: P
authors.lastname: Francescato
authors.forename: A
authors.initials: A
authors.lastname: Fraccari
authors.forename: I
authors.initials: I
authors.lastname: Galatulas
mesh_terms: D000595:Amino Acid Sequence; D000970:Antineoplastic Agents / Q000138:chemical synthesis* / Q000494:pharmacology; D006367:HeLa Cells; D006801:Humans; D008969:Molecular Sequence Data; D010455:Peptides / Q000138:chemical synthesis* / Q000494:pharmacology
chemical_list: D000970:Antineoplastic Agents; D010455:Peptides