IgE responses in the serum and gastric lymph of sheep infected with Teladorsagia circumcincta.
The IgE response of naive or previously infected sheep to 50,000 infective larvae of Teladorsagia circumcincta was monitored in serum and gastric lymph using a monoclonal antibody generated to recombinant ovine IgE in a dot blot assay. In 4/5 naive sheep, lymph and serum IgE concentrations increased from days 8 and 14 after infection, respectively. In most previously infected sheep, the IgE response to challenge was more rapid, although not necessarily greater than that following a primary infection. IgE concentrations in lymph were some 4-fold higher than in serum indicating that its source was the mucosa or draining nodes.
Huntley
J F
JF
Moredun Research Institute, Edinburgh, Scotland, UK.
Schallig
H D
HD
Kooyman
F N
FN
MacKellar
A
A
Millership
J
J
Smith
W D
WD
D000818:Animals; D000909:Antibodies, Helminth / Q000032:analysis* / Q000097:blood; D000911:Antibodies, Monoclonal; D015153:Blotting, Western; D015151:Immunoblotting; D007073:Immunoglobulin E / Q000032:analysis* / Q000097:blood; D008196:Lymph / Q000276:immunology*; D009349:Nematode Infections / Q000097:blood / Q000276:immunology* / Q000469:parasitology; D011994:Recombinant Proteins / Q000032:analysis / Q000097:blood; D012756:Sheep; D012757:Sheep Diseases / Q000097:blood / Q000276:immunology* / Q000469:parasitology; D013270:Stomach; D013997:Time Factors
D000909:Antibodies, Helminth; D000911:Antibodies, Monoclonal; D011994:Recombinant Proteins; D007073:Immunoglobulin E