title: The role of ionic strength on the enhancement of the hemolytic activity of sticholysin I, a cytolysin from Stichodactyla helianthus.
abstract: Sticholysin I (St I) is a potent cytolytic polypeptide purified from the Caribbean sea anemone Stichodactyla helianthus. The hemolytic activity of sticholysin is potentiated by its preincubation at high ionic strengths. In the present work the mechanism of the potentiating action of the medium ionic strength on the toxin hemolytic capacity is investigated. It is suggested that preincubation with high saline concentration induces a transition of St I to a more relaxed conformation that facilitates the lytic process.
authors.affiliation: Departamento de Bíochímica, Facultad de Biología, Universidad de La Habana, Cuba.
authors.forename: C
authors.initials: C
authors.lastname: Alvarez
authors.forename: M E
authors.initials: ME
authors.lastname: Lanio
authors.forename: M
authors.initials: M
authors.lastname: Tejuca
authors.forename: D
authors.initials: D
authors.lastname: Martínez
authors.forename: F
authors.initials: F
authors.lastname: Pazos
authors.forename: A M
authors.initials: AM
authors.lastname: Campos
authors.forename: M V
authors.initials: MV
authors.lastname: Encinas
authors.forename: T
authors.initials: T
authors.lastname: Pertinhez
authors.forename: S
authors.initials: S
authors.lastname: Schreier
authors.forename: E A
authors.initials: EA
authors.lastname: Lissi
mesh_terms: D000818:Animals; D002942:Circular Dichroism; D003064:Cnidarian Venoms / Q000737:chemistry / Q000494:pharmacology*; D006460:Hemolysin Proteins / Q000494:pharmacology*; D009994:Osmolar Concentration; D011487:Protein Conformation; D012615:Sea Anemones / Q000737:chemistry*; D013050:Spectrometry, Fluorescence
chemical_list: D003064:Cnidarian Venoms; D006460:Hemolysin Proteins