A simplified sample preparation method from various foods for PCR detection of pathogenic Yersinia enterocolitica: a possible model for other food pathogens.
A simplified method for the direct application of multiplex polymerase chain reaction (PCR) was developed to detect plasmid-bearing virulent serotypes of Yersinia enterocolitica (YEP+) in a variety of foods. Strains of YEP+ representing five serotypes were detected in enriched swab samples of artificially contaminated pork chops, ground pork, cheese and zucchini using multiplex PCR analysis. The method was also effective for identifying YEP+ strains in naturally contaminated porcine tongues. The use of swabs eliminated time-consuming extraction of DNA from food, inhibition of PCR by food-derived DNA, interference by background flora and reduced the time needed for processing samples. The detection of other food pathogens should be feasible by this technique.
Bhaduri
S
S
Microbial Food Safety Research Unit, Eastern Regional Research Center, USDA, Wyndmoor, PA 19038, USA.
Cottrell
B
B
D000818:Animals; D002611:Cheese / Q000382:microbiology; D019662:Cucurbitaceae / Q000382:microbiology; D004269:DNA, Bacterial / Q000302:isolation & purification; D005516:Food Microbiology; D008461:Meat Products / Q000382:microbiology; D008954:Models, Biological; D016133:Polymerase Chain Reaction / Q000379:methods*; D012703:Serotyping; D013552:Swine; D015009:Yersinia Infections / Q000175:diagnosis / Q000382:microbiology*; D015008:Yersinia enterocolitica / Q000235:genetics* / Q000302:isolation & purification / Q000472:pathogenicity*
D004269:DNA, Bacterial