title: An evolutionarily conserved gene on human chromosome 5q33-q34, UBH1, encodes a novel deubiquitinating enzyme.
abstract: While cloning breakpoint sequences of a leukemia patient exhibiting a t(5; 14) translocation, we identified a pseudogenic variant of a novel multigene family in proximity to the breakpoint. Chromosomal in situ hybridization suggested that the gene family is clustered on human chromosome 5q33-q34. The gene family is evolutionarily conserved. Northern blot analysis of mouse tissues revealed low-level expression of a functional member of this gene family in almost all samples. Marked levels of transcripts were detected by in situ hybridization in the retina, the olfactory epithelium, the peripheral neuronal ganglia, and distinct areas of the gut. The predicted protein displays striking similarity to a hypothetical protein of Caenorhabditis elegans (R10E11.3.) and to two yeast deubiquitinating enzymes, Ubp9 and Ubp13, albeit to a lesser extent. We expressed the putative coding region of the human gene in Escherichia coli and demonstrated that it indeed bears deubiquitinating activity based on its ability to cleave ubiquitin from a ubiquitin-beta-galactosidase fusion protein. This new deubiquitinating enzyme has been named UBH1, for ubiquitin hydrolyzing enzyme 1.
authors.affiliation: Department of Pediatrics II, University of Ulm, Germany.
authors.forename: T E
authors.initials: TE
authors.lastname: Hansen-Hagge
authors.forename: J W
authors.initials: JW
authors.lastname: Janssen
authors.forename: H
authors.initials: H
authors.lastname: Hameister
authors.forename: F R
authors.initials: FR
authors.lastname: Papa
authors.forename: U
authors.initials: U
authors.lastname: Zechner
authors.forename: T
authors.initials: T
authors.lastname: Seriu
authors.forename: A
authors.initials: A
authors.lastname: Jauch
authors.forename: D
authors.initials: D
authors.lastname: Becke
authors.forename: M
authors.initials: M
authors.lastname: Hochstrasser
authors.forename: C R
authors.initials: CR
authors.lastname: Bartram
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D017173:Caenorhabditis elegans / Q000201:enzymology / Q000235:genetics; D002874:Chromosome Mapping; D002883:Chromosomes, Human, Pair 14; D002895:Chromosomes, Human, Pair 5; D017124:Conserved Sequence; D017931:DNA Primers; D010450:Endopeptidases / Q000096:biosynthesis / Q000737:chemistry / Q000235:genetics*; D019143:Evolution, Molecular; D006801:Humans; D017403:In Situ Hybridization; D007938:Leukemia / Q000235:genetics; D051379:Mice; D008969:Molecular Sequence Data; D005810:Multigene Family; D009420:Nervous System / Q000196:embryology / Q000378:metabolism; D009474:Neurons / Q000201:enzymology; D016133:Polymerase Chain Reaction; D011544:Pseudogenes; D012441:Saccharomyces cerevisiae / Q000201:enzymology / Q000235:genetics; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid; D014158:Transcription, Genetic; D014178:Translocation, Genetic; D043222:Ubiquitin Thiolesterase
chemical_list: D017931:DNA Primers; C486402:USP12 protein, human; D010450:Endopeptidases; D043222:Ubiquitin Thiolesterase; C486401:Usp12 protein, mouse