Ultrastructural effects of the chelating agent 1,10-phenanthroline on Trypanosoma cruzi epimastigotes in vitro.
The in vitro effects of the metal chelator 1,10-phenanthroline (OPHEN) on the ultrastructure of Trypanosoma cruzi epimastigotes were investigated. Epimastigotes treated with OPHEN display swelling and electron-dense deposits in the kinetoplast, mitochondrion, and cisternae of the endoplasmic reticulum. These morphological alterations are dose-dependent and first appear at an OPHEN concentration of 5.0 microg/ml. Analytical electron microscope examination indicates that the metallic portion of the electron-dense deposits is predominantly calcium.
Lane
J E
JE
Department of Biology, Vanderbilt University, Nashville, TN 37235, USA.
Bogitsh
B J
BJ
Ribeiro-Rodrigues
R
R
Kral
M V
MV
Jones
M M
MM
Carter
C E
CE
D000818:Animals; D002614:Chelating Agents / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D008854:Microscopy, Electron; D010618:Phenanthrolines / Q000494:pharmacology*; D014349:Trypanosoma cruzi / Q000187:drug effects* / Q000254:growth & development / Q000648:ultrastructure*
D002614:Chelating Agents; D010618:Phenanthrolines; C025205:1,10-phenanthroline