Differences in the inhibition of neuritic outgrowth in organotypic cultures of rat foetal dorsal root ganglia treated with cisplatin and carboplatin: a comparative study.
Neurotoxic effect of cisplatin and carboplatin, two anticancer drugs with different neurotoxic potential, was examined in organotypic cultures of rat foetal dorsal root ganglia (DRG), maintained in a semisolid agar culture medium. Two main parameters of neurites growing out of the ganglia--the mean radial length of neurites and the area of neurite outgrowth--were used to evaluate the toxic influence of both drugs. IC50 values were calculated from the dose-response curves established at three cultivation time points (24, 48, and 72 h). Cisplatin demonstrated higher toxicity in comparison with carboplatin for all exposure times. The lowest concentration which caused neurite outgrowth alteration was 25 microM of carboplatin and 10 microM of cisplatin. IC 50 was 11.93, 8.25, 8.58 microM for cisplatin and 177.46, 47.83, 44.94 for carboplatin after 24, 48 and 72 h, respectively.
Jirsová
K
K
Laboratory of Cell Pathology, Institute of Experimental Medicine, Academy of Sciences, Prague, Czech Republic.
Mandys
V
V
D000818:Animals; D000970:Antineoplastic Agents / Q000494:pharmacology*; D016190:Carboplatin / Q000494:pharmacology*; D002945:Cisplatin / Q000494:pharmacology*; D005260:Female; D005727:Ganglia, Spinal / Q000166:cytology / Q000187:drug effects* / Q000254:growth & development*; D016501:Neurites / Q000187:drug effects*; D009924:Organ Culture Techniques; D011247:Pregnancy; D051381:Rats; D013997:Time Factors
D000970:Antineoplastic Agents; D016190:Carboplatin; D002945:Cisplatin