title: Characterization of acellular dermal matrices (ADMs) prepared by two different methods.
abstract: The efficacy of acellular dermal matrix (ADM) in the treatment of full-thickness skin injuries as a dermal substitute depends on its low antigenicity, capacity for rapid vascularization, and stability as a dermal template. These properties will be determined largely by the final composition of the ADM. We have treated human skin with either Dispase followed by Triton X-100 detergent or NaCl followed by SDS detergent, cryosectioned the resulting ADMs, and then characterized them immunohistochemically. Staining for cell-associated antigens (HLA-ABC, HLA-DR, vimentin, desmin, talin), extracellular matrix components (chondroitin sulfate, fibronectin, laminin, vitronectin, hyaluronic acid), elastin, and collagen type VII was dramatically reduced or absent from ADMs prepared by both methods. However, significant amounts of elastin, keratan sulfate, laminin, and collagen types III and IV were still observed in both ADMs. Both methods of ADM preparation resulted in extensive extraction of both cellular and extracellular components of the skin but retention of the basic dermal architecture. In general, ADM prepared by the NaCl-SDS method retained larger amounts of each antigen than did that prepared by the Dispase-Triton method. This was most evident for laminin and type VII collagen but larger amounts of type IV collagen, fibronectin, desmin, elastin, and HLA-DR were also evident in the NaCl-SDS ADM.
authors.affiliation: Department of Surgery, Cook County Hospital, Chicago, IL 60612, USA.
authors.forename: R J
authors.initials: RJ
authors.lastname: Walter
authors.forename: T
authors.initials: T
authors.lastname: Matsuda
authors.forename: H M
authors.initials: HM
authors.lastname: Reyes
authors.forename: J M
authors.initials: JM
authors.lastname: Walter
authors.forename: M
authors.initials: M
authors.lastname: Hanumadass
mesh_terms: D003598:Cytoskeletal Proteins / Q000378:metabolism; D010450:Endopeptidases / Q000494:pharmacology; D005109:Extracellular Matrix / Q000378:metabolism*; D016326:Extracellular Matrix Proteins / Q000378:metabolism; D006025:Glycosaminoglycans / Q000378:metabolism; D006680:HLA Antigens / Q000378:metabolism; D006801:Humans; D007124:Immunoenzyme Techniques; D017830:Octoxynol / Q000494:pharmacology; D012867:Skin / Q000187:drug effects* / Q000378:metabolism*; D012965:Sodium Chloride / Q000494:pharmacology; D012967:Sodium Dodecyl Sulfate / Q000494:pharmacology*
chemical_list: D003598:Cytoskeletal Proteins; D016326:Extracellular Matrix Proteins; D006025:Glycosaminoglycans; D006680:HLA Antigens; D012967:Sodium Dodecyl Sulfate; D012965:Sodium Chloride; D017830:Octoxynol; D010450:Endopeptidases; C023139:dispase