Genetic transformation of cassava (Manihot esculenta Crantz).
Genetic engineering can be used to complement traditional breeding methods in crop plant improvement. Transfer of genes from heterologous species provides the means of selectively introducing new traits into crop plants and expanding the gene pool beyond what has been available to traditional breeding systems. The prerequisites for genetic engineering are efficient transformation and tissue culture systems that allow selection and regeneration of transgenic plants. Cassava, an integral plant for food security in developing countries, has until now been recalcitrant to transformation approaches. We report here a method for regenerating stably transformed cassava plants after cocultivation with Agrobacterium tumefaciens, which opens cassava for future improvement via biotechnology.
Li
H Q
HQ
Inst. of Plant Sciences, Swiss Federal Institute of Technology, ETH Zentrum, Zürich, Switzerland.
Sautter
C
C
Potrykus
I
I
Puonti-Kaerlas
J
J
D016960:Agrobacterium tumefaciens / Q000502:physiology; D019470:Biolistics; D002365:Manihot / Q000196:embryology / Q000235:genetics*; D009693:Nucleic Acid Hybridization; D030821:Plants, Genetically Modified; D012639:Seeds; D014170:Transformation, Genetic