Cyanine fluorochrome-labeled antibodies in vivo: assessment of tumor imaging using Cy3, Cy5, Cy5.5, and Cy7.
Monoclonal antibodies to two different targetable antigens were conjugated to each of four commercially available cyanine fluorochromes. Equal amounts of all four antibodies were coinjected into tumor-bearing animals and imaged. Small, superficial tumors were adequately labeled using all four fluorochromes. Large tumors were labeled well only by Cy7, probably due to self-masking and dilution effects. Cy7 was superior to other cyanine fluorochromes for visualizing structures located deep within the animal.
Ballou
B
B
Center for Light Microscope Imaging and Biotechnology, Carnegie Mellon University, Pittsburgh, PA 15213, USA.
Fisher
G W
GW
Deng
J S
JS
Hakala
T R
TR
Srivastava
M
M
Farkas
D L
DL
D000818:Animals; D000911:Antibodies, Monoclonal; D000951:Antigens, Neoplasm / Q000378:metabolism; D052160:Benzothiazoles; D002232:Carbocyanines; D005456:Fluorescent Dyes; D006801:Humans; D051379:Mice; D008807:Mice, Inbred BALB C; D010750:Phosphoproteins / Q000378:metabolism; D016601:RNA-Binding Proteins / Q000378:metabolism; D018243:Teratocarcinoma / Q000737:chemistry / Q000473:pathology; D014407:Tumor Cells, Cultured; D000097796:Nucleolin
D000911:Antibodies, Monoclonal; D000951:Antigens, Neoplasm; D052160:Benzothiazoles; D002232:Carbocyanines; D005456:Fluorescent Dyes; D010750:Phosphoproteins; D016601:RNA-Binding Proteins; D000097796:Nucleolin; C098793:CY5.5 cyanine dye; C089187:cyanine dye 3; C085321:cyanine dye 5; C011204:cyanine dye 7