Bovine seminal platelet-activating factor acetylhydrolase: association properties in seminal plasma and with lipoproteins.
The enzyme responsible for most of the phospholipase A2 (PLA2) activity present in bovine seminal plasma was recently purified to homogeneity. Sequencing revealed that the enzyme is also a platelet-activating factor acetylhydrolase (PAF-AH) of the serum type with kinetic properties generally similar to its serum homologue. In the present work, we have attempted to clarify its physiological function by studying its association properties in seminal plasma. As was observed previously for its PLA2 activity, its PAF-AH activity was also inhibited by the major proteins of bovine seminal plasma (BSP proteins). Sequential dilution experiments as well as centrifuging semen on Percoll did not reveal detectable association of PAF-AH with spermatozoa. Neither did the enzyme interact with lipid particles reported to be present in bovine seminal plasma. The purified PAF-AH, however, did display lipoprotein association properties in vitro similar to those demonstrated by the serum enzyme in vivo. At pH 7.4, it could associate with both low density lipoproteins and very low density lipoproteins but not with high density lipoproteins. Overall the data presented here indicate that the enzyme is strongly inactivated as a PAF-AH in seminal plasma and that it does not associate with lipid particles or spermatozoa.
Soubeyrand
S
S
Department of Medicine, University of Montreal, Montreal, Que., Canada.
Thérien
I
I
Manjunath
P
P
D043203:1-Alkyl-2-acetylglycerophosphocholine Esterase; D000818:Animals; D002417:Cattle; D004791:Enzyme Inhibitors / Q000494:pharmacology; D006863:Hydrogen-Ion Concentration; D008074:Lipoproteins / Q000378:metabolism*; D008077:Lipoproteins, LDL / Q000378:metabolism; D008079:Lipoproteins, VLDL / Q000378:metabolism; D008297:Male; D010741:Phospholipases A / Q000037:antagonists & inhibitors / Q000378:metabolism*; D054467:Phospholipases A2; D029584:Prostatic Secretory Proteins; D011506:Proteins / Q000494:pharmacology; D012661:Semen / Q000201:enzymology*; D029589:Seminal Plasma Proteins; D013094:Spermatozoa / Q000378:metabolism
D004791:Enzyme Inhibitors; D008074:Lipoproteins; D008077:Lipoproteins, LDL; D008079:Lipoproteins, VLDL; D029584:Prostatic Secretory Proteins; D011506:Proteins; D029589:Seminal Plasma Proteins; C043076:beta-microseminoprotein; D010741:Phospholipases A; D054467:Phospholipases A2; D043203:1-Alkyl-2-acetylglycerophosphocholine Esterase