Mutation analysis of the putative tumor suppressor PTEN/MMAC1 in human ovarian cancer.
A putative tumor suppressor, PTEN/MMAC1 gene was recently identified at 10q23 and was found to be frequently altered in several types of human tumors. To determine the possible role of this gene in human ovarian tumors, we screened 31 unselected primary epithelial ovarian cancers with matched normal tissues and 7 ovarian cancer cell lines. Two homozygous deletions (4.8%) were detected in primary ovarian tumors by a semi-quantitative multiplex polymerase chain reaction. However, no point mutations were observed in any of the PTEN/MMAC1 coding sequences as determined by denaturing high-performance liquid chromatography and DNA sequencing. These data indicate that PTEN/MMAC1 mutations are rare in ovarian cancers.
Yokomizo
A
A
Division of Experimental Pathology, Department of Laboratory Medicine and Pathology and Department of Urology Research, Mayo Clinic/Foundation, Rochester, MN 55905, USA.
Tindall
D J
DJ
Hartmann
L
L
Jenkins
R B
RB
Smith
D I
DI
Liu
W
W
D017398:Alternative Splicing; D002879:Chromosomes, Human, Pair 10; D004252:DNA Mutational Analysis; D005091:Exons; D005260:Female; D016147:Genes, Tumor Suppressor; D006801:Humans; D007438:Introns; D010051:Ovarian Neoplasms / Q000235:genetics*; D051059:PTEN Phosphohydrolase; D010744:Phosphoric Monoester Hydrolases; D011110:Polymorphism, Genetic; D017027:Protein Tyrosine Phosphatases / Q000235:genetics*; D014407:Tumor Cells, Cultured; D025521:Tumor Suppressor Proteins
D025521:Tumor Suppressor Proteins; D010744:Phosphoric Monoester Hydrolases; D017027:Protein Tyrosine Phosphatases; D051059:PTEN Phosphohydrolase; C494929:PTEN protein, human