Structural investigations of basic amphipathic model peptides in the presence of lipid vesicles studied by circular dichroism, fluorescence, monolayer and modeling.
A cationic amphiphilic peptide made of 10 leucine and 10 lysine residues, and four of its fluorescent derivatives in which leucines were substituted by Trp residues at different locations on the primary sequence have been synthesized. The interactions of these five peptides with neutral anionic or cationic vesicles were investigated using circular dichroism, steady state and time-resolved fluorescence with a combination of Trp quenching by brominated lipid probes, monolayers, modeling with minimization and simulated annealing procedures. We show that all the five peptides interact with neutral and anionic DMPC, DMPG, DOPC or egg yolk PC vesicles. The binding takes place whatever the peptide conformation in solution is. In the case of DMPC bilayers the binding free energy DeltaG is estimated at -8 kcal mole-1 and the number of phospholipid molecules involved is about 20-25 per peptide molecule. Peptides are bound as single-stranded alpha helices orientated parallel to the bilayer surface. In the anchoring of phospholipid head groups around the peptides, the lipid molecules are not smeared out in a plane parallel to the membrane surface but are organized around the hydrophilic face of the alpha helices like 'wheat grains around an ear' and protrude outside the bilayer towards the solvent. We suggest that such a lipid arrangement generates transient structural defects responsible for the membrane permeability enhancement. When an electrical potential is applied, the axis of the peptide helices remains parallel to the membrane surface and does not reorient to give rise to a bundle of helix monomers that forms transmembrane channels via a 'barrel stave' mechanism. The penetration depth of alpha helices in relation to the position of phosphorus atoms in the unperturbed lipid leaflet is estimated at 3.2 A.
Mangavel
C
C
Centre de Biophysique Moléculaire-CNRS-Rue Charles-Sadron, 45071 Orleans Cedex 2, France.
Maget-Dana
R
R
Tauc
P
P
Brochon
J C
JC
Sy
D
D
Reynaud
J A
JA
D000595:Amino Acid Sequence; D000838:Anions; D002412:Cations; D002942:Circular Dichroism; D008081:Liposomes / Q000737:chemistry*; D008564:Membrane Potentials; D008567:Membranes, Artificial; D008956:Models, Chemical; D008958:Models, Molecular; D008969:Molecular Sequence Data; D010455:Peptides / Q000138:chemical synthesis* / Q000737:chemistry; D010713:Phosphatidylcholines / Q000737:chemistry; D011485:Protein Binding; D013050:Spectrometry, Fluorescence; D013501:Surface-Active Agents / Q000737:chemistry*; D013816:Thermodynamics; D014364:Tryptophan / Q000737:chemistry
D000838:Anions; D002412:Cations; D008081:Liposomes; D008567:Membranes, Artificial; D010455:Peptides; D010713:Phosphatidylcholines; D013501:Surface-Active Agents; D014364:Tryptophan; C017251:1,2-oleoylphosphatidylcholine