Influence of diet-modified lipoprotein fractions from rabbits on eicosanoid production and on proliferation of rabbit skin fibroblasts.
Three groups of rabbits received for 8 weeks either ordinary pellets, fish oil-enriched pellets or a cholesterol-rich (0.5%) diet for modification of lipoprotein composition. At the end of the dietary period, low density lipoproteins (LDL) and high density lipoproteins (HDL) were prepared from plasma of these rabbits. Rabbit skin fibroblasts (RSF) in culture were exposed to these isolated LDL and HDL fractions or to human serum albumin (HSA), respectively. Eicosanoid release was estimated after stimulation of cells with the Ca ionophore A 23187. Exogenous added HDL from the pellet group and HDL from the cholesterol-fed group as well as HSA stimulated PGI2 production by rabbit skin fibroblasts (P < 0.05, respectively) as compared to controls. All LDL preparations investigated inhibited PGI2 release from RSF (P < 0.05, respectively). The TXA2 release from RSF was increased only by HDL from cholesterol-fed rabbits and by HSA (P < 0.05, respectively). Pretreatment of RSF with cholesterol significantly decreased the number of detectable cells. Subsequent addition of HDL from the pellet or from the fish oil group completely restored the ability of cholesterol-enriched RSF to proliferate (P < 0.05, respectively).
Beitz
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Martin Luther University Halle-Wittenberg, School of Medicine, Halle, Germany.
D000818:Animals; D002455:Cell Division / Q000187:drug effects; D002478:Cells, Cultured; D002784:Cholesterol / Q000494:pharmacology; D002791:Cholesterol, Dietary / Q000008:administration & dosage; D008076:Cholesterol, HDL / Q000097:blood / Q000187:drug effects; D004032:Diet; D015777:Eicosanoids / Q000378:metabolism*; D011464:Epoprostenol / Q000378:metabolism; D005347:Fibroblasts / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D005395:Fish Oils / Q000008:administration & dosage; D008075:Lipoproteins, HDL / Q000737:chemistry / Q000494:pharmacology*; D008077:Lipoproteins, LDL / Q000737:chemistry / Q000494:pharmacology*; D008079:Lipoproteins, VLDL / Q000097:blood / Q000187:drug effects; D011817:Rabbits; D012709:Serum Albumin / Q000494:pharmacology; D012867:Skin / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism; D013928:Thromboxane A2 / Q000378:metabolism; D013929:Thromboxane B2 / Q000378:metabolism
D002791:Cholesterol, Dietary; D008076:Cholesterol, HDL; D015777:Eicosanoids; D005395:Fish Oils; D008075:Lipoproteins, HDL; D008077:Lipoproteins, LDL; D008079:Lipoproteins, VLDL; D012709:Serum Albumin; D013929:Thromboxane B2; D013928:Thromboxane A2; D002784:Cholesterol; D011464:Epoprostenol