Expression of prostaglandin E2 receptor subtypes in the developing sheep brainstem.
Expression in sheep brain of EP transcripts and their ontogeny in the brainstem was determined using RT-PCR. Sheep EP subtypes showed high homology (>80%) with other species. EP1, EP2, EP3 and EP4 were expressed in spinal cord, medulla, pons, diencephalon, hippocampus, pituitary, cortex and cerebellum of the fetal, newborn and adult sheep brain. Expression of all four peaked in the newborn, whereas fetal and adult expression were similar. A similar ontogeny suggests a common regulation.
Tai
T C
TC
Institute of Medical Science, University of Toronto, Toronto, ON, Canada.
Lye
S J
SJ
Adamson
S L
SL
D000367:Age Factors; D000818:Animals; D000831:Animals, Newborn; D001933:Brain Stem / Q000737:chemistry* / Q000196:embryology* / Q000502:physiology; D017931:DNA Primers; D005260:Female; D005333:Fetus / Q000502:physiology; D018507:Gene Expression Regulation, Developmental; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D011247:Pregnancy; D012333:RNA, Messenger / Q000032:analysis; D018078:Receptors, Prostaglandin E / Q000235:genetics*; D012756:Sheep
D017931:DNA Primers; D012333:RNA, Messenger; D018078:Receptors, Prostaglandin E