Treatment of rat gliomas with recombinant retrovirus harboring Herpes simplex virus thymidine kinase suicide gene.
The retrovirus vector containing Herpes simplex virus type 1 thymidine kinase (HSVtk) gene was constructed. The vector was transfected into the packaging cell line PG13. It was shown that individual transfected cells differ in the production of recombinant retrovirus and in their susceptibility to be killed by ganciclovir. Recombinant retrovirus with a gibbon envelope was able to transduce the HSVtk gene into rat glioma cells. In vivo studies confirmed the ability of intraperitoneal ganciclovir administration to influence subcutaneous and intracerebral tumors developed after injection of C6 rat glioma cells with subsequent injection of HSVtk retrovirus producing cells.
Hlavatý
J
J
Cancer Research Institute, Slovak Academy of Sciences, Bratislava.
Hlubinová
K
K
Altanerová
V
V
Líska
J
J
Altaner
C
C
D000818:Animals; D015774:Ganciclovir / Q000008:administration & dosage*; D015316:Genetic Therapy; D005822:Genetic Vectors; D005910:Glioma / Q000628:therapy*; D007274:Injections, Intraperitoneal; D009368:Neoplasm Transplantation; D051381:Rats; D017207:Rats, Sprague-Dawley; D017208:Rats, Wistar; D011995:Recombination, Genetic; D012190:Retroviridae / Q000235:genetics; D018139:Simplexvirus / Q000201:enzymology / Q000235:genetics*; D013937:Thymidine Kinase / Q000235:genetics*; D014162:Transfection; D014407:Tumor Cells, Cultured
D013937:Thymidine Kinase; D015774:Ganciclovir