Genotypic characterization of human immunodeficiency virus type 1 in Greece. Multicentre Study on HIV-1 Heterogeneity.
The HIV-1 subtype distribution in 83 HIV-1-seropositive individuals living in Greece was investigated by using the heteroduplex mobility assay (HMA), DNA sequencing, and phylogenetic analysis. The results revealed that partial HIV-1 gp120 sequences from 71 (86%) patients were subtype B, 5 (6%) were subtype A, 4 were subtype D (5%), 2 (2%) were subtype C, and 1 (1%) was subtype I. The subtype I isolate was documented in an intravenous drug user. A high prevalence (90-100%) of B isolates among intravenous drug users, hemophiliacs, and homosexual men was observed, in contrast to heterosexuals, among whom non-B subtypes seemed to be common (42.9%, p < 0.001). Among the Greek population subtype B is the most frequent (94%), in contrast to the high prevalence (57%) of non-B isolates found in emigrants living in Greece (p < 0.001). A heterosexual transmission case of subtype D in a Greek individual not traveling abroad was also documented. The broad HIV-1 diversity in Greece may be explained by population movements, such as migration and traveling.
Nasioulas
G
G
National Retrovirus Reference Center, Department of Hygiene and Epidemiology, Athens University Medical School, Greece.
Paraskevis
D
D
Paparizos
V
V
Lazanas
M
M
Karafoulidou
A
A
Hatzakis
A
A
D000328:Adult; D004279:DNA, Viral / Q000097:blood; D004641:Emigration and Immigration; D005260:Female; D005838:Genotype; D006115:Greece / Q000453:epidemiology; D015699:HIV Envelope Protein gp120 / Q000235:genetics; D006679:HIV Seropositivity / Q000453:epidemiology / Q000635:transmission / Q000821:virology*; D015497:HIV-1 / Q000235:genetics*; D018451:Homosexuality, Male; D006801:Humans; D008297:Male; D008875:Middle Aged; D017720:Molecular Epidemiology; D009692:Nucleic Acid Heteroduplexes; D010802:Phylogeny; D016133:Polymerase Chain Reaction / Q000379:methods; D015995:Prevalence; D017422:Sequence Analysis, DNA; D015819:Substance Abuse, Intravenous
D004279:DNA, Viral; D015699:HIV Envelope Protein gp120; D009692:Nucleic Acid Heteroduplexes