A PCR specific for Escherichia coli O157 based on the rfb locus encoding O157 lipopolysaccharide.
A PCR was developed for the detection of Escherichia coli O157 based on the rfbE O-antigen synthesis genes. A 479-bp PCR product was amplified specifically from E. coli O157 in cell lysates containing 200 or 2 CFU following crude DNA extraction. The PCR detected < 1 CFU of E. coli O157 per ml in raw milk following enrichment.
Desmarchelier
P M
PM
Commonwealth Scientific and Industrial Research Organisation, Brisbane, Australia. Patricia.Desmarchelier@foodscience.afisc.csiro.au
Bilge
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Fegan
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Mills
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Vary
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Tarr
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D000818:Animals; D015373:Bacterial Typing Techniques; D002238:Carbohydrate Epimerases / Q000235:genetics*; D004797:Enzyme-Linked Immunosorbent Assay; D019453:Escherichia coli O157 / Q000235:genetics / Q000302:isolation & purification*; D006801:Humans; D007118:Immunoassay; D008892:Milk / Q000382:microbiology*; D019081:O Antigens / Q000096:biosynthesis*; D016133:Polymerase Chain Reaction / Q000379:methods*; D012680:Sensitivity and Specificity; D012703:Serotyping; D000637:Transaminases / Q000235:genetics*
D019081:O Antigens; D000637:Transaminases; D002238:Carbohydrate Epimerases; C061272:perosamine synthetase