Immune complex transfer two-site chemiluminescent immunoassay for serum growth hormone in alevin chum salmon.
An immune complex transfer two-site chemiluminescent immunoassay (CLIA) for salmon growth hormone (GH) was developed to measure serum GH in alevin chum salmon (Oncorhynchus keta) using a chemiluminescent acridinium ester as a label. The immune complex transfer method dramatically reduced non-specifically bound of acridinium ester labelled antibody without a decrease in the specific binding. Consequently, we could detect lower levels of GH than achieved previously in a two-site CLIA for salmon GH. The detection limit of the assay was 7.8 fg/ml and the standard curve was linear up to 250 fg/ml. Coefficients of variation were 2.2-7.7% within-assay and 5.3-91% between-assay. We have developed a highly sensitive and reproducible GH method and applied it to measurement of GH in alevin chum salmon.
Fukada
H
H
Department of Biology and Aquaculture, Faculty of Fisheries, Hokkaido University, Japan.
Ban
M
M
Chiba
H
H
Hara
A
A
D000166:Acridines; D000704:Analysis of Variance; D000818:Animals; D000936:Antigen-Antibody Complex; D013006:Growth Hormone / Q000097:blood* / Q000276:immunology / Q000592:standards; D007118:Immunoassay / Q000379:methods* / Q000592:standards / Q000706:statistics & numerical data; D008163:Luminescent Measurements; D017685:Oncorhynchus keta / Q000097:blood*; D012015:Reference Standards
D000166:Acridines; D000936:Antigen-Antibody Complex; D013006:Growth Hormone