title: Human immunodeficiency virus type 1 vectors efficiently transduce human hematopoietic stem cells.
abstract: Lentiviruses are potentially advantageous compared to oncoretroviruses as gene transfer agents because they can infect nondividing cells. We demonstrate here that human immunodeficiency virus type 1 (HIV-1)-based vectors were highly efficient in transducing purified human hematopoietic stem cells. Transduction rates, measured by marker gene expression or by PCR of the integrated provirus, exceeded 50%, and transduction appeared to be independent of mitosis. Derivatives of HIV-1 were constructed to optimize the vector, and a deletion of most of Vif and Vpr was required to ensure the long-term persistence of transduced cells with relatively stable expression of the marker gene product. These results extend the utility of this lentivirus vector system.
authors.affiliation: Department of Biochemistry and Howard Hughes Medical Institute, Stanford University Medical Center, Stanford, California 94305, USA. sutton@cmgm.stanford.edu
authors.forename: R E
authors.initials: RE
authors.lastname: Sutton
authors.forename: H T
authors.initials: HT
authors.lastname: Wu
authors.forename: R
authors.initials: R
authors.lastname: Rigg
authors.forename: E
authors.initials: E
authors.lastname: Böhnlein
authors.forename: P O
authors.initials: PO
authors.lastname: Brown
mesh_terms: D016346:Gene Products, vif / Q000502:physiology; D016336:Gene Products, vpr / Q000502:physiology; D018014:Gene Transfer Techniques; D005822:Genetic Vectors; D015497:HIV-1 / Q000235:genetics*; D006412:Hematopoietic Stem Cells / Q000378:metabolism*; D006801:Humans; D014779:Virus Replication; D054320:vif Gene Products, Human Immunodeficiency Virus; D054325:vpr Gene Products, Human Immunodeficiency Virus
chemical_list: D016346:Gene Products, vif; D016336:Gene Products, vpr; D054320:vif Gene Products, Human Immunodeficiency Virus; D054325:vpr Gene Products, Human Immunodeficiency Virus