title: Enhanced hybridization labeling signals in Southern blotted DNAs fractionated with voltage gradient gel electrophoresis.
abstract: An enhancement of hybridization labeling signals is demonstrated in Southern blotted DNAs, fractionated by voltage gradient gel electrophoresis. This enhancement is due to a reduced thickness of each single nucleic acid band in the gel as a consequence of the gradient effect, corresponding to an increased concentration of DNA per unit area.
authors.affiliation: Dipartimento di Biologia Cellulare e dello Sviluppo, Università di Palermo, Italy.
authors.forename: R
authors.initials: R
authors.lastname: Barbieri
authors.forename: G
authors.initials: G
authors.lastname: Duro
authors.forename: V
authors.initials: V
authors.lastname: Izzo
mesh_terms: D000818:Animals; D015139:Blotting, Southern / Q000379:methods*; D005591:Chemical Fractionation; D004247:DNA / Q000737:chemistry*; D004563:Electrochemistry; D004587:Electrophoresis, Agar Gel / Q000379:methods*; D008565:Membrane Proteins / Q000235:genetics; D009693:Nucleic Acid Hybridization; D012617:Sea Urchins
chemical_list: C065555:Bep4 protein, Paracentrotus lividus; D008565:Membrane Proteins; D004247:DNA