Isolation and partial characterization of a lectin from Artocarpus incisa L. seeds.
A lectin was isolated from the saline extract of Artocarpus incisa seed by affinity chromatography on cross-linked Adenanthera pavonina galactomannan in 0.15 M NaCl. The lectin was also retained in a D-gal-agarose resin and had no requirements for divalent metal cations (Ca2+ and Mn2+) for activity. The lectin contains 2.1% of carbohydrate and is characterized by high contents of acidic and hydroxylated amino acids. The lectin presented two protein bands in SDS-PAGE, with M(r) 15.5 and 12 kDa, respectively, and contains no alpha-helix, 64% antiparallel beta-sheet and 21% parallel beta-sheet/beta-turn. When submitted to gel filtration in Superose 12 R (FPLC) and Superdex 75 HR 5/5 (HPLC) columns, the lectin showed an M(r) of 48-49 kDa, suggesting a tetrameric structure.
Moreira
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RA
Departamento de Bioquímica e Biologia Molecular, Universidade Federal do Ceará, Brazil.
Castelo-Branco
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CC
Monteiro
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AC
Tavares
R O
RO
Beltramini
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LM
D000596:Amino Acids / Q000032:analysis; D002118:Calcium / Q000737:chemistry; D002846:Chromatography, Affinity; D002850:Chromatography, Gel; D002852:Chromatography, Ion Exchange; D002942:Circular Dichroism; D004591:Electrophoresis, Polyacrylamide Gel; D004912:Erythrocytes / Q000187:drug effects; D006386:Hemagglutination Tests; D006801:Humans; D007525:Isoelectric Focusing; D037102:Lectins / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D008345:Manganese / Q000737:chemistry; D037121:Plant Lectins; D010944:Plants / Q000737:chemistry*; D017433:Protein Structure, Secondary; D012639:Seeds / Q000737:chemistry*
D000596:Amino Acids; D037102:Lectins; D037121:Plant Lectins; D008345:Manganese; D002118:Calcium