title: Three genes encode distinct AP33 proteins involved in Trichomonas vaginalis cytoadherence.
abstract: Adherence to host cells is essential for the initiation and maintenance of infection by mucosal pathogens. The protozoan Trichomonas vaginalis colonizes the human urogenital tract via four surface proteins (AP65, AP51, AP33 and AP23). To characterize AP33 further, six cDNA clones were examined. Restriction mapping indicated that the six clones represented three similar genes. Southern analysis confirmed the existence of three single-copy AP33 genes and suggested a semi-conservative genomic arrangement between T. vaginalis isolates. Analysis of full-length sequences determined that each contained a 930bp open reading frame encoding a protein of approximately 33,000 Da. Sequence comparisons revealed a high degree of identity at both the DNA and the protein levels. N-terminal protein sequencing established the presence of leader peptides. Each of the three full-length recombinant proteins had a predicted pI of approximately 10, which was verified experimentally for the T. vaginalis AP33 adhesin. A database search revealed that AP33 had significant identity to the succinyl-CoA synthetase alpha-subunit of several different organisms and virtually 100% identity to the reported T. vaginalis subunit. Unlike commercially purchased enzyme, the recombinant proteins retained adhesive properties equal to the natural T. vaginalis AP33. The characteristics of the AP33 protein are similar to those of the other adhesins and emphasize a complex host-parasite relationship.
authors.affiliation: NIDR/NIH, Bethesda, MD 20892-4370, USA.
authors.forename: J A
authors.initials: JA
authors.lastname: Engbring
authors.forename: J F
authors.initials: JF
authors.lastname: Alderete
mesh_terms: D000818:Animals; D001483:Base Sequence; D015139:Blotting, Southern; D015815:Cell Adhesion Molecules / Q000235:genetics*; D004591:Electrophoresis, Polyacrylamide Gel; D004926:Escherichia coli / Q000235:genetics; D017125:Genes, Protozoan / Q000235:genetics*; D006801:Humans; D015151:Immunoblotting; D008565:Membrane Proteins / Q000737:chemistry / Q000235:genetics*; D008969:Molecular Sequence Data; D016366:Open Reading Frames / Q000235:genetics; D015800:Protozoan Proteins / Q000235:genetics*; D051381:Rats; D015183:Restriction Mapping; D017386:Sequence Homology, Amino Acid; D013389:Succinate-CoA Ligases / Q000145:classification / Q000235:genetics; D014246:Trichomonas vaginalis / Q000737:chemistry* / Q000145:classification / Q000235:genetics*
chemical_list: C113260:AP33 protein, Trichomonas vaginalis; D015815:Cell Adhesion Molecules; D008565:Membrane Proteins; D015800:Protozoan Proteins; D013389:Succinate-CoA Ligases