title: [Serum HRP-2 antigens and imported Plasmodium falciparum malaria: comparison of ParaSight-F and ICT malaria P.f].
abstract: Serum levels of HRP-2 antigens against Plasmodium falciparum in 568 venous blood samples collected at two general hospitals were evaluated using the ParaSight-F (Becton-Dickinson) alone (568/568 samples) or in combination (156/568 samples) with the new ICT-Malaria P.f. (ICT-diagnostic). Comparison with the reference method (thin and thick blood smears assessed by two experienced parasitologists) showed that both tests were highly sensitive (93% and 96% respectively) and specific (98% and 98% respectively). The positive predictive values of the two tests were equal (96%) and the negative predicative values were close (96% and 98% respectively). Although both tests provided results within 10 minutes and required no special equipment for interpretation, the ICT-Malaria P.f. test seemed simpler and easier to use than the ParaSight-F test. The ParaSight-F test alone was used to monitor serum antigen levels after treatment in 24 patients. Antigen levels remained positive for at least three days after disappearance of circulating parasites (range: 3 to 28 days). Evaluation of serum levels of HRP-2 antigens can be useful for emergency diagnosis, especially in patients with low circulating parasite levels. The ICT-Malaria P.f. test seems especially suited for in-field use.
authors.affiliation: Laboratoire de Biologie, l'Hôpital d'Instruction des Armées Bégin, Saint Mandé, France.
authors.forename: J D
authors.initials: JD
authors.lastname: Cavallo
authors.forename: E
authors.initials: E
authors.lastname: Hernandez
authors.forename: P
authors.initials: P
authors.lastname: Gerome
authors.forename: N
authors.initials: N
authors.lastname: Plotton
authors.forename: T
authors.initials: T
authors.lastname: Debord
authors.forename: R
authors.initials: R
authors.lastname: Le Vagueresse
mesh_terms: D000818:Animals; D000953:Antigens, Protozoan / Q000097:blood*; D006801:Humans; D008288:Malaria / Q000097:blood; D016778:Malaria, Falciparum / Q000097:blood*; D016780:Malaria, Vivax / Q000097:blood; D008889:Military Personnel; D010961:Plasmodium / Q000145:classification / Q000276:immunology; D010963:Plasmodium falciparum / Q000276:immunology*; D010966:Plasmodium vivax / Q000276:immunology; D011237:Predictive Value of Tests; D011446:Prospective Studies; D011506:Proteins / Q000032:analysis*; D011933:Reagent Kits, Diagnostic; D012680:Sensitivity and Specificity; D000099053:Histidine-Rich Glycoprotein
chemical_list: D000953:Antigens, Protozoan; D011506:Proteins; D011933:Reagent Kits, Diagnostic; D000099053:Histidine-Rich Glycoprotein
vernacular_title: Antigénémie HRP-2 et paludisme d'importation à Plasmodium falciparum: comparaison du ParaSight-F et de l'ICT malaria P.f.