title: Measurement of the levels of reticulated platelets after plateletpheresis to monitor activity of thrombopoiesis.
abstract: BACKGROUND
  Little is known about the effects of long-term plateletpheresis on the donors' health. The aim of this study was to examine the effect of plateletpheresis on the time course of reticulated platelet counts as an estimate for thrombopoiesis.
  
  
  METHODS
  The effect of moderate platelet depletion on the thrombopoietic capacity was evaluated prospectively by the measurement of reticulated platelets before and after plateletpheresis and on the following 4 days. Donors undergoing plateletpheresis for the first time were compared to those donating platelets every other week for more than 18 months.
  
  
  RESULTS
  The median levels of reticulated platelets were significantly lower in frequent donors than in new donors. In new donors, there was a transient increase in the median levels of reticulated platelets on Day 3 after apheresis, and baseline values were reached again on Day 4. On the contrary, in repeat donors, there was a sustained rise in the percentage of reticulated platelets from Days 1 through 4. However, this increase in reticulated platelet counts was still less than that seen in new donors. There was no difference in the peripheral blood platelet counts in the two groups at any time point.
  
  
  CONCLUSIONS
  These findings suggest that repeat platelet donation might lead to a relative exhaustion of thrombopoiesis, as evidenced by the low levels of reticulated platelets exhibited by repeat donors. The reticulated platelet count can be used to monitor the thrombopoietic capacity of long-term platelet donors.
authors.affiliation: Clinic for Blood Group Serology and Transfusion Medicine, University of Vienna, Austria.
authors.forename: P
authors.initials: P
authors.lastname: Stohlawetz
authors.forename: G
authors.initials: G
authors.lastname: Stiegler
authors.forename: B
authors.initials: B
authors.lastname: Jilma
authors.forename: M
authors.initials: M
authors.lastname: Dettke
authors.forename: P
authors.initials: P
authors.lastname: Höcker
authors.forename: S
authors.initials: S
authors.lastname: Panzer
mesh_terms: D000328:Adult; D000367:Age Factors; D001792:Blood Platelets / Q000166:cytology*; D002455:Cell Division; D005260:Female; D006410:Hematopoiesis / Q000502:physiology*; D006801:Humans; D008297:Male; D008875:Middle Aged; D010976:Platelet Count; D010983:Plateletpheresis; D013997:Time Factors