Interaction between hepatitis delta virus-encoded proteins and hepatitis B virus envelope protein domains.
Hepatitis delta virus (HDV) packaging requires prenylation of the HDV large protein (p27), as well as a direct protein-protein interaction between HDV proteins and hepatitis B virus (HBV) envelope protein domains. To investigate this interaction, we have analysed the binding capacity of baculovirus-expressed delta p24 and p27 proteins to synthetic peptides specific for the HBV envelope. Although a higher degree of binding was observed with p27, both p24 and p27 could bind HBV envelope peptides. One such peptide corresponded to residues 56-80 located in the cytosolic loop of the small HBV envelope protein, and another corresponded to 23 carboxy-terminal residues of the pre-S1 specific to the large HBV envelope protein. This indicates that in addition to p27, p24 may contribute to packaging of HDV through a protein-protein interaction with HBV envelope domains, and that an interaction between the pre-S1 polypeptide and delta proteins may play a role in infectivity.
Hourioux
C
C
Laboratoire de Virologie EP CNRS 117, Faculté de Médecine, Tours, France.
Sureau
C
C
Poisson
F
F
Brand
D
D
Goudeau
A
A
Roingeard
P
P
D000595:Amino Acid Sequence; D000818:Animals; D001665:Binding Sites; D002460:Cell Line; D018963:Hepatitis Antigens / Q000235:genetics / Q000378:metabolism*; D006514:Hepatitis B Surface Antigens / Q000378:metabolism*; D006515:Hepatitis B virus / Q000378:metabolism*; D003698:Hepatitis Delta Virus / Q000378:metabolism*; D035923:Hepatitis delta Antigens; D006801:Humans; D008969:Molecular Sequence Data; D011498:Protein Precursors / Q000378:metabolism*; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000378:metabolism; D018411:Spodoptera; D013329:Structure-Activity Relationship
D018963:Hepatitis Antigens; D006514:Hepatitis B Surface Antigens; D035923:Hepatitis delta Antigens; D011498:Protein Precursors; D011993:Recombinant Fusion Proteins; C442244:hepatitis delta virus large antigen; C050806:presurface protein 1, hepatitis B surface antigen; C050807:presurface protein 2, hepatitis B surface antigen