title: Photoinduced electron transfer from carboxymethylated cytochrome c to plastocyanin.
abstract: Photoinduced electron transfer from cytochrome c to plastocyanin was investigated using a novel method. Reduced carboxymethylated cytochrome c (CmCyt c), with carbon monoxide bound to the heme iron, and oxidized plastocyanin were mixed. At 1 mM CO the reduced state of CmCyt c is stabilized by about 350 meV. After flash photolysis of CO the apparent redox potential of CmCyt c drops resulting in electron transfer to plastocyanin. The electron transfer characteristics were investigated at approximately 30 different wavelengths in the range 390-460 nm. A global fit of the data showed that the electron transfer rate is 960+/-30 s-1 at pH 7.
authors.affiliation: Department of Biochemistry and Biophysics, University of Göteborg and Chalmers University of Technology (Medicinaregatan 9C), P.O. Box 462, SE-405 30 Göteborg, Sweden.
authors.forename: M
authors.initials: M
authors.lastname: Karpefors
authors.forename: M T
authors.initials: MT
authors.lastname: Wilson
authors.forename: P
authors.initials: P
authors.lastname: Brzezinski
mesh_terms: D003574:Cytochrome c Group / Q000737:chemistry*; D008027:Light; D010782:Photolysis; D010970:Plastocyanin / Q000737:chemistry*
chemical_list: D003574:Cytochrome c Group; D010970:Plastocyanin