[Rehabilitation of the hepatocyte glycogen-forming function in the rat cirrhotic liver due to carbohydrate rich diet].
By cytofluorometric and biochemical methods the content of total glycogen and its fractions was investigated on the smears of isolated liver cells: labile fraction (LF) and stable fraction (SF) and also activities of glycogen phosphorylase (GP), glucose-6-phosphatase (G-6-Pase) and glycogen synthase. The material was obtained from serial liver biopsies from each investigated animal prior to CCl4 action (control), with cirrhosis (6 months of CCl4 poisoning) and 1, 3 and 6 months after CCl4 poisoning was finished. It was shown that chronic CCl4 poisoning induced a typical liver cirrhosis accompanied with the 2-3 times increase in the total glycogen content, in comparison with the norm, with the decrease in LF to 53%, and also with the fall of G-6-Pase and GP activities by 82 and 25%, resp. After 1, 3 and 6 months following poisoning cessation, the lobule structure, infringed due to cirrhosis, was not restored. But functional parameters of the cirrhotic liver were seen gradually recovering without CCl4 poisoning. The application of carbohydrate rich diet favoured a most complete rehabilitation: the content of total glycogen and its fractions and the activity of G-6-Pase and GP returned to the normal level.
Kudriavtseva
M V
MV
Institute of Cytology, Russian Academy of Sciences, St. Petersburg.
Emel'ianov
A V
AV
Sakuta
G A
GA
Bezborodkina
N N
NN
Kudriavtsev
B N
BN
D000818:Animals; D002252:Carbon Tetrachloride Poisoning / Q000150:complications / Q000378:metabolism / Q000628:therapy*; D004040:Dietary Carbohydrates / Q000627:therapeutic use*; D005434:Flow Cytometry; D006003:Glycogen / Q000096:biosynthesis*; D008099:Liver / Q000378:metabolism* / Q000473:pathology; D008106:Liver Cirrhosis, Experimental / Q000139:chemically induced / Q000378:metabolism / Q000628:therapy*; D008297:Male; D051381:Rats
D004040:Dietary Carbohydrates; D006003:Glycogen
Reabilitatsiia glikogenoobrazovatel'noĭ funktsii gepatotsitov tsirroticheski izmenennoĭ pecheni krys v usloviiakh pitaniia vysokouglevodnoĭ dietoĭ.