Pantothenol protects rats against some deleterious effects of gamma radiation.
Rats were exposed to gamma radiation from a 60Co source, receiving 0.25 Gy at weekly intervals. During 2 d before each irradiation, the animals received daily intragastric doses of 26 mg pantothenol or 15 mg beta-carotene per kg body weight. One hour after the third irradiation session, the animals were killed and their livers were analyzed. In animals not supplied with pantothenol, the irradiation resulted in a significant decrease of total liver lipids and a 50% decrease in phospholipids. Liver cholesterol was decreased by about 20%. Irradiation produced lipid peroxidation as expressed by doubling of the amounts of conjugated dienes and ketone dienes and of thiobarbituric acid reactive compounds. The amount of CoA in liver was decreased by 24% and that of reduced glutathione by 40%. The NAD+/NADH ratio was increased by 60% and the activity of NADP-dependent malate dehydrogenase (decarboxylating) was decreased by 26%. The amount of pantothenic acid and its derivatives (expressed as pantolactone-generating compounds) in blood decreased by about 80%. In rats to which pantothenol was administered, the content of pantothenic acid in blood was tripled compared to nonirradiated (control) rats, and all the biochemical parameters measured in liver were the same as in nonirradiated animals.
Slyshenkov
V S
VS
Institute of Biochemistry, Academy of Sciences of Belarus, Grodno.
Omelyanchik
S N
SN
Moiseenok
A G
AG
Trebukhina
R V
RV
Wojtczak
L
L
D000818:Animals; D000975:Antioxidants; D002784:Cholesterol / Q000032:analysis / Q000528:radiation effects; D003065:Coenzyme A / Q000032:analysis / Q000528:radiation effects; D004334:Drug Administration Schedule; D005260:Female; D005720:Gamma Rays / Q000009:adverse effects*; D005978:Glutathione / Q000096:biosynthesis / Q000737:chemistry / Q000528:radiation effects; D019803:Glutathione Disulfide / Q000096:biosynthesis / Q000737:chemistry / Q000528:radiation effects; D007441:Intubation, Gastrointestinal; D019344:Lactic Acid / Q000032:analysis / Q000528:radiation effects; D008055:Lipids / Q000032:analysis / Q000528:radiation effects; D008099:Liver / Q000737:chemistry / Q000187:drug effects* / Q000528:radiation effects*; D008291:Malate Dehydrogenase / Q000032:analysis / Q000528:radiation effects; D050538:Malate Dehydrogenase (NADP+); D009243:NAD / Q000032:analysis / Q000528:radiation effects; D010205:Pantothenic Acid / Q000031:analogs & derivatives* / Q000097:blood / Q000494:pharmacology; D010743:Phospholipids / Q000032:analysis / Q000528:radiation effects; D011506:Proteins / Q000737:chemistry; D019289:Pyruvic Acid / Q000032:analysis / Q000528:radiation effects; D011837:Radiation-Protective Agents / Q000494:pharmacology; D051381:Rats; D011919:Rats, Inbred Strains; D017382:Reactive Oxygen Species; D017392:Thiobarbituric Acid Reactive Substances / Q000032:analysis / Q000528:radiation effects; D019207:beta Carotene / Q000008:administration & dosage / Q000494:pharmacology
D000975:Antioxidants; D008055:Lipids; D010743:Phospholipids; D011506:Proteins; D011837:Radiation-Protective Agents; D017382:Reactive Oxygen Species; D017392:Thiobarbituric Acid Reactive Substances; D019207:beta Carotene; D009243:NAD; D010205:Pantothenic Acid; C007288:dexpanthenol; D019344:Lactic Acid; D019289:Pyruvic Acid; D002784:Cholesterol; D008291:Malate Dehydrogenase; D050538:Malate Dehydrogenase (NADP+); D005978:Glutathione; D003065:Coenzyme A; D019803:Glutathione Disulfide