Toxicological evaluation of sobatum.
Sobatum, the partially purified component of the plant Solanum trilobatum was obtained from the petroleum ether/ethyl acetate (75:25) extractable portion. It was identified as beta-sitosterol by comparison with an authentic sample and proved to be an anticancer agent by in vitro and in vivo experiments. The aim of the present study is to evaluate the acute/subacute toxicity of sobatum and its effect on antioxidant enzymes. In the acute toxicity test, there was a single exposure of sobatum in mice in order to evaluate toxicity symptoms. In the subacute toxicity study, the exposure was usually daily so as to evaluate the target organs affected by the compound and the major toxic effects and enzymatic and histopathological changes. The results of the study suggested that sobatum did not induce any toxic symptoms or death immediately after injection or at the end of the experimental period. It was also shown that there was no change in blood parameters or antioxidant enzymes in sobatum-treated animals. Regarding histopathological evaluation, there was no development of gross abnormalities or pathological lesions observed in any of the tissues in sobatum-treated and control groups. Hence, the present study concluded that the anticancer agent, sobatum, did not produce any acute/subacute toxicities or anomalies in the synthesis of antioxidant enzymes.
Mohanan
P V
PV
Toxicology Group, Biomedical Technology Wing, Sree Chitra Tirunal Institute for Medical Sciences and Technology, Kerala, India.
Devi
K S
KS
D000410:Alanine Transaminase / Q000187:drug effects; D000469:Alkaline Phosphatase / Q000187:drug effects; D000818:Animals; D000970:Antineoplastic Agents / Q000633:toxicity*; D001219:Aspartate Aminotransferases / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D005260:Female; D006321:Heart / Q000187:drug effects; D007668:Kidney / Q000187:drug effects; D008099:Liver / Q000187:drug effects; D008168:Lung / Q000187:drug effects; D008297:Male; D051379:Mice; D010936:Plant Extracts / Q000633:toxicity*; D013154:Spleen / Q000187:drug effects
D000970:Antineoplastic Agents; D010936:Plant Extracts; C110896:Sobatum; D001219:Aspartate Aminotransferases; D000410:Alanine Transaminase; D000469:Alkaline Phosphatase