title: A high efficiency technique for the generation of transgenic sugar beets from stomatal guard cells.
abstract: An optimized protocol has been developed for the efficient and rapid genetic modification of sugar beet (Beta vulgaris L.). A polyethylene glycol-mediated DNA transformation technique could be applied to protoplast populations enriched specifically for a single totipotent cell type derived from stomatal guard cells, to achieve high transformation frequencies. Bialaphos resistance, conferred by the pat gene, produced a highly efficient selection system. The majority of plants were obtained within 8 to 9 weeks and were appropriate for plant breeding purposes. All were resistant to glufosinate-ammonium-based herbicides. Detailed genomic characterization has verified transgene integration, and progeny analysis showed Mendelian inheritance.
authors.affiliation: Department of Cell Biology, DLO-Centre for Plant Breeding and Reproduction Research (CPRO-DLO), Wageningen, The Netherlands. r.d.hall@cpro.dlo.nl
authors.forename: R D
authors.initials: RD
authors.lastname: Hall
authors.forename: T
authors.initials: T
authors.lastname: Riksen-Bruinsma
authors.forename: G J
authors.initials: GJ
authors.lastname: Weyens
authors.forename: I J
authors.initials: IJ
authors.lastname: Rosquin
authors.forename: P N
authors.initials: PN
authors.lastname: Denys
authors.forename: I J
authors.initials: IJ
authors.lastname: Evans
authors.forename: J E
authors.initials: JE
authors.lastname: Lathouwers
authors.forename: M P
authors.initials: MP
authors.lastname: Lefèbvre
authors.forename: J M
authors.initials: JM
authors.lastname: Dunwell
authors.forename: A
authors.initials: A
authors.lastname: van Tunen
authors.forename: F A
authors.initials: FA
authors.lastname: Krens
mesh_terms: D001709:Biotechnology; D019663:Chenopodiaceae / Q000166:cytology / Q000235:genetics* / Q000378:metabolism; D003433:Crosses, Genetic; D004351:Drug Resistance / Q000235:genetics; D005818:Genetic Engineering; D006540:Herbicides / Q000494:pharmacology; D009943:Organophosphorus Compounds / Q000494:pharmacology; D030821:Plants, Genetically Modified; D010957:Plasmids / Q000235:genetics; D013395:Sucrose / Q000378:metabolism; D014170:Transformation, Genetic
chemical_list: D006540:Herbicides; D009943:Organophosphorus Compounds; C003122:bialaphos; D013395:Sucrose