title: Effects of two large doses of equine recombinant growth hormone on clinical, haematological and serum biochemical variables in adult horses.
abstract: OBJECTIVE
  To evaluate the clinical, haematological, and serum biochemical effects of two large doses of recombinant equine growth hormone.
  
  
  METHODS
  Duplicated Latin square.
  
  
  METHODS
  Three Thoroughbred and three Standardbred mares aged between 12 and 17 years.
  
  
  METHODS
  Two horses were randomly assigned into one of three groups. On each of three successive days, each horse pair received one of two dosages of growth hormone or a saline placebo so that by the end of the experiment all three horse pairs had received both dosages and the saline placebo. Dose rates selected were 50 micrograms/kg, and 100 micrograms/kg. A clinical examination was performed and a venous blood sample drawn for a complete blood count and serum biochemical analysis before administration of growth hormone and at 1, 2, 3, 4, 6, 8 and 24 h after injection. Data were analysed by a repeated measures analysis of variance assessing the effects of dose and time.
  
  
  RESULTS
  There was an effect of time on a number of clinical, haematological, and serum biochemical variables. There were significant effects of growth hormone on heart rate and serum glucose concentration but values for both variables remained within the reference range.
  
  
  CONCLUSIONS
  The results of the present study suggest that equine recombinant growth hormone has a wide margin of safety and show that the single administration of up to five times the recommended dose rate has no significant effects on clinical, haematological, or serum biochemical variables.
authors.affiliation: Department of Veterinary Clinical Sciences, University of Sydney, Camden, New South Wales.
authors.forename: A J
authors.initials: AJ
authors.lastname: Dart
authors.forename: M
authors.initials: M
authors.lastname: Strong
authors.forename: R J
authors.initials: RJ
authors.lastname: Rose
authors.forename: D R
authors.initials: DR
authors.lastname: Hodgson
mesh_terms: D000818:Animals; D001774:Blood Chemical Analysis / Q000662:veterinary; D001831:Body Temperature / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D005260:Female; D005340:Fibrinogen / Q000032:analysis / Q000187:drug effects; D013006:Growth Hormone / Q000494:pharmacology*; D006339:Heart Rate / Q000187:drug effects; D006400:Hematocrit / Q000662:veterinary; D006403:Hematologic Tests / Q000662:veterinary; D006454:Hemoglobins / Q000032:analysis / Q000187:drug effects; D006736:Horses / Q000097:blood* / Q000502:physiology; D007958:Leukocyte Count / Q000662:veterinary; D011994:Recombinant Proteins / Q000494:pharmacology; D012119:Respiration / Q000187:drug effects
chemical_list: D006454:Hemoglobins; D011994:Recombinant Proteins; D005340:Fibrinogen; D013006:Growth Hormone