Trehalose 6-phosphate production with energy coupling fermentation by yeast cells.
We tried a method for the production of trehalose 6-phosphate (T6P) with energy-coupling fermentation by baker's yeast. T6P was produced in a reaction mixture containing glucose, 5'-UMP, MgSO4, inorganic phosphate, and dried cells of baker's yeast as the enzyme preparation, T6P was isolated from the reaction mixture and identified by TLC, HPLC, GC-MS, and enzymatic methods. The reaction conditions suitable for T6P production were investigated. The formation of T6P and its precursors, glucose 6-phosphate and UDPglucose, at various pHs and concentrations of substrates was examined. Accumulation of T6P was maximum with a reaction mixture containing 1 M glucose, 20 mM 5'-UMP, 20 mM MgSO4, 400 mM sodium phosphate buffer (pH 6.2), and 100 mg/ml dried cells of baker's yeast shaken at 37 degrees C for 6 h. The yield of T6P as a percentage of glucose was 11% (mol/mol) under these reaction conditions.
Doi
J
J
Department of Food Science and Nutrition, Nara Women's University, Japan.
Yokoigawa
K
K
Isobe
Y
Y
Kawai
H
H
D000249:Adenosine Monophosphate / Q000378:metabolism; D003470:Culture Media; D004734:Energy Metabolism; D005285:Fermentation / Q000502:physiology*; D005947:Glucose / Q000494:pharmacology; D006863:Hydrogen-Ion Concentration; D008278:Magnesium Sulfate / Q000494:pharmacology; D010710:Phosphates / Q000494:pharmacology; D012441:Saccharomyces cerevisiae / Q000378:metabolism*; D013403:Sugar Phosphates / Q000096:biosynthesis* / Q000737:chemistry / Q000302:isolation & purification; D014199:Trehalose / Q000031:analogs & derivatives* / Q000096:biosynthesis / Q000737:chemistry / Q000302:isolation & purification; D014542:Uridine Monophosphate / Q000378:metabolism
D003470:Culture Media; D010710:Phosphates; D013403:Sugar Phosphates; D000249:Adenosine Monophosphate; C082722:trehalose-6-phosphate; D008278:Magnesium Sulfate; D014199:Trehalose; D014542:Uridine Monophosphate; D005947:Glucose