Tandemly repeated genomic sequence demonstrates inter- and intra-strain genetic variation in Schistosoma japonicum.
Genetic variability within and among four geographical strains of Schistosoma japonicum was examined using a novel repetitive element. The element, termed Sirh1.0, was isolated from genomic DNA of a Philippine strain of S. japonicum using a combination of restriction fragment PCR and band-stab PCR. Sjrh1.0 is a tandemly repeated element, the sequence of which appears to be species-specific, in that it hybridized to DNA from S. japonicum but not to DNA from S. mansoni. Its sequence does not match previously deposited sequences in GenBank. When employed as a probe in Southern hybridization analysis, radiolabelled Sjrh1.0 revealed sex-specific and strain-specific differences in genomic DNA of individual worms. We also found individual genetic variation within geographical isolates of the Asian schistosome.
Drew
A C
AC
Molecular Parasitology Unit, Queensland Institute of Medical Research, Brisbane, Australia.
Brindley
P J
PJ
Lewis
F A
FA
Liang
Y S
YS
Minchella
D J
DJ
D000818:Animals; D001483:Base Sequence; D018509:DNA, Helminth / Q000032:analysis*; D005260:Female; D014644:Genetic Variation; D008297:Male; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D012091:Repetitive Sequences, Nucleic Acid / Q000235:genetics*; D012549:Schistosoma japonicum / Q000235:genetics*; D016415:Sequence Alignment; D012737:Sex Factors
D018509:DNA, Helminth