[Inhibitory effects of shimotsu-to, a traditional Chinese herbal prescription, on ultraviolet radiation-induced cell damage and prostaglandin E2 release in cultured Swiss 3T3 cells].
We have investigated the effects of Shimotsu-to, a traditional Chinese herbal prescription (Kampo medicine), on ultraviolet (UV) radiation-induced cell damage and prostaglandin E2 (PGE2) release in cultured Swiss 3T3 cells to examine the anti-inflammatory mechanism of Shimotsu-to. Short-term UV irradiation significantly induced cell damage and stimulated PGE2 release in Swiss 3T3 cells cultured for 4 h. The UV-radiation-induced cell damage and the stimulation of PGE2 release were significantly suppressed by the treatment with Shimotsu-to. Among the single crude drug components of Shimotsu-to, Rehmanniae Radix showed significant protective effects against UV-radiation-induced cell damage and PGE2 release. Other synthetic anti-inflammatory agents, such as dexamethasone, dipotassium glycyrrhizinate and allantoin also protected against UV-induced cell damage and inhibited PGE2 release in cultured Swiss 3T3 cells. These results suggest that the anti-inflammatory mechanism of Shimotsu-to against UV-irradiated erythema (acute skin inflammation) in guinea pigs in vivo may be mediated by the inhibition of PGE2 release from cutaneous target cells.
Sakuma
K
K
Faculty of Pharmaceutical Sciences, Josai University, Saitama, Japan.
Ogawa
M
M
Kimura
M
M
Yamamoto
K
K
Ogihara
M
M
D016475:3T3 Cells / Q000378:metabolism* / Q000528:radiation effects*; D000818:Animals; D000893:Anti-Inflammatory Agents / Q000494:pharmacology*; D002478:Cells, Cultured; D003864:Depression, Chemical; D015232:Dinoprostone / Q000378:metabolism*; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D051379:Mice; D014466:Ultraviolet Rays
D000893:Anti-Inflammatory Agents; D004365:Drugs, Chinese Herbal; C079710:shimotsu-to; D015232:Dinoprostone