Characterization of yhcN, a new forespore-specific gene of Bacillus subtilis.
A new Bacillus subtilis sporulation-specific gene, yhcN, has been identified, the expression of which is dependent on the forespore-specific sigma factor sigmaG and to a much lesser extent on sigmaF. A translational yhcN-lacZ fusion is expressed at a very high level in the forespore, and the protein encoded by yhcN was detected in the inner spore membrane. A yhcN mutant sporulates normally and yhcN spores have identical resistance properties to wild-type spores. However, the outgrowth of yhcN spores is slower than that of wild-type spores.
Bagyan
I
I
Department of Biochemistry, University of Connecticut Health Center, Farmington, CT 06032, USA.
Noback
M
M
Bron
S
S
Paidhungat
M
M
Setlow
P
P
D000595:Amino Acid Sequence; D001412:Bacillus subtilis / Q000235:genetics* / Q000254:growth & development / Q000502:physiology*; D001426:Bacterial Proteins / Q000096:biosynthesis / Q000737:chemistry / Q000235:genetics; D001483:Base Sequence; D004926:Escherichia coli / Q000235:genetics; D015964:Gene Expression Regulation, Bacterial; D005798:Genes, Bacterial; D008969:Molecular Sequence Data; D016254:Mutagenesis, Insertional; D011401:Promoter Regions, Genetic; D012808:Sigma Factor / Q000096:biosynthesis / Q000737:chemistry / Q000235:genetics / Q000502:physiology; D013171:Spores, Bacterial / Q000737:chemistry / Q000235:genetics; D014157:Transcription Factors / Q000096:biosynthesis / Q000737:chemistry / Q000235:genetics / Q000502:physiology
D001426:Bacterial Proteins; D012808:Sigma Factor; D014157:Transcription Factors; C063202:sporulation-specific sigma factors; C113113:yhcN protein, Bacillus subtilis