Responses of Halobacterium halobium and Sulfolobus solfataricus to hydrogen peroxide and N-methyl-N'-nitro-N-nitrosoguanidine [correction of N-methyl-N-nitrosoguanidine] exposure.
We have examined the capacity of Halobacterium halobium and Sulfolobus solfataricus to withstand the lethal effect of hydrogen peroxide and N-methyl-N'-nitro-N-nitrosoguanidine [corrected].  We tested a variety of pretreatment regimens with both mutagens and all failed to elicit an inducible response to the lethal effects of those compounds in either organism. We have observed AP endonuclease activity in protein extracts from both organisms. In addition, S. solfataricus extracts contain activities that remove 3-methyl-adenine and O(6)-methyl-guanine from methylated DNA. This is the first report of these DNA repair activities in any member of the Archaea.
Prail
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Department of Biochemistry and Molecular Biology, The Pennsylvania State University, University Park, PA 16802, USA.
Taylor
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D019757:Carbon-Oxygen Lyases / Q000032:analysis; D004260:DNA Repair; D019617:Halobacterium salinarum / Q000187:drug effects* / Q000201:enzymology; D006861:Hydrogen Peroxide / Q000494:pharmacology*; D008769:Methylnitronitrosoguanidine / Q000494:pharmacology*; D009153:Mutagens / Q000494:pharmacology*; D016944:Sulfolobus / Q000187:drug effects* / Q000201:enzymology
D009153:Mutagens; D008769:Methylnitronitrosoguanidine; D006861:Hydrogen Peroxide; D019757:Carbon-Oxygen Lyases