HLA-DQB1 and DRB1 alleles in Egyptian children with steroid-sensitive nephrotic syndrome.
Steroid-sensitive nephrotic syndrome (SSNS) of children has been associated with several HLA-DR and DQ alleles. To investigate this association in Egyptian children, 27 patients with SSNS were typed for HLA-DRB1 and DQB1 alleles using DNA polymerase chain-reverse hybridization technique. The results were compared with 121 healthy subjects for HLA-DRB1 and 59 subjects for DQB1 alleles. We found that: (1) patients have higher frequencies of both DQB1 *0601 (81.5% vs. 10.2% in controls, Pc=0.0001) and DRB1 *01 (44.4% vs. 3.3% in controls, Pc=0.00003). Their relative risks are significantly high [38.9, confidence interval (CI)=10.7-140.7, and 23.4, CI=6.7-81.9, respectively]; (2) the frequency of DRB1 *11 alleles was low in SSNS patients (3.75% vs. 32.2% in controls), but was not significant when P was corrected (P=0.005, Pc=NS). These findings suggest that DQB1 *0601 and DRBI *01 or closely associated unknown genes confer susceptibility to SSNS. However, further studies with larger numbers of patients are needed.
Bakr
A M
AM
Department of Pediatrics, Mansoura Faculty of Medicine, Egypt.
El-Chenawy
F
F
D000305:Adrenal Cortex Hormones / Q000494:pharmacology*; D000483:Alleles; D002648:Child; D002675:Child, Preschool; D005260:Female; D006683:HLA-DQ Antigens / Q000235:genetics*; D059866:HLA-DQ beta-Chains; D006684:HLA-DR Antigens / Q000235:genetics*; D059811:HLA-DRB1 Chains; D006801:Humans; D008297:Male; D009404:Nephrotic Syndrome / Q000235:genetics*
D000305:Adrenal Cortex Hormones; D006683:HLA-DQ Antigens; D059866:HLA-DQ beta-Chains; C069051:HLA-DQB1 antigen; D006684:HLA-DR Antigens; D059811:HLA-DRB1 Chains