title: Stopped-flow analysis of Ru(bpy)3(3+) chemiluminescent reactions.
abstract: The stopped-flow technique was employed to measure chemiluminescent emission from the reaction of a mixture of oxalate and proline with a chemiluminescence reagent, tris(2,2'-bipyridine) ruthenium(III), or Ru(bpy)3(3+) is a versatile reagent and is often used in bioanalytical applications, including the detection of certain drugs and their metabolites, for example. Unfortunately, Ru(bpy)3(3+) has not yet been fully examined as a possible chemiluminescence reagent for simultaneous kinetic determinations. In this work, a differential reaction rate method, based on simple least squares regressions of the pseudo-first order decay data, was used to resolve two compounds, oxalate and proline, reacting simultaneously with Ru(bpy)3(3+). Our results indicate that stopped-flow analyses with Ru(bpy)3(3+) could provide a viable method for simultaneous determinations of unresolvable analytes of environmental and pharmaceutical importance.
authors.affiliation: University of Illinois at Urbana-Champaign, Department of Chemistry, 61801, USA.
authors.forename: L L
authors.initials: LL
authors.lastname: Shultz
authors.forename: T A
authors.initials: TA
authors.lastname: Nieman
mesh_terms: D015082:2,2'-Dipyridyl / Q000031:analogs & derivatives* / Q000737:chemistry; D007202:Indicators and Reagents; D007700:Kinetics; D008163:Luminescent Measurements; D009942:Organometallic Compounds / Q000737:chemistry*; D010070:Oxalates / Q000737:chemistry; D019815:Oxalic Acid; D011392:Proline / Q000737:chemistry; D012428:Ruthenium / Q000737:chemistry*
chemical_list: D007202:Indicators and Reagents; D009942:Organometallic Compounds; D010070:Oxalates; C058901:tris(2,2'-bipyridine)ruthenium III; D015082:2,2'-Dipyridyl; D012428:Ruthenium; D011392:Proline; D019815:Oxalic Acid