Simple one-tube reverse transcription-polymerase chain reaction protocol containing anticontamination procedure for detection of GB virus C/hepatitis G virus RNA.
A simple one-tube reverse transcription-polymerase chain reaction protocol for detection of GB virus C/hepatitis G virus (GBV-C/HGV) RNA in serum samples containing anticontamination uracil-N-glycosylase procedure was developed. Amplification products were detected in a standard microtiter plate format using a commercial PCR ELISA kit (Boehringer Mannheim, Germany). Complete agreement between the results of a novel method and the previously determined GBV-C/HGV RNA status was obtained on 131 serum samples (76 GBV-C/HGV RNA positive and 55 GBV-C/HGV RNA negative samples). The novel method provides the potential for automated, simple, rapid and accurate routine detection of GBV-C/HGV RNA in diagnostic virological laboratories.
Poljak
M
M
Institute of Microbiology and Immunology, Medical Faculty of Ljubljana, Slovenia. poljak@ibmi.mf.uni-lj.si
Seme
K
K
D018067:Flaviviridae / Q000235:genetics / Q000302:isolation & purification*; D006801:Humans; D016133:Polymerase Chain Reaction / Q000295:instrumentation / Q000379:methods*; D012367:RNA, Viral / Q000302:isolation & purification*; D015203:Reproducibility of Results
D012367:RNA, Viral