title: Histidine carboxylase of Leuconostoc oenos 9204: purification, kinetic properties, cloning and nucleotide sequence of the hdc gene.
abstract: Histidine decarboxylase (HDC) was purified to homogeneity from Leuconostoc oenos 9204, a wine lactic acid bacterium. Histidine decarboxylase comprised two subunits, respectively alpha and beta. The hdc gene was cloned and sequenced. The gene encodes a single polypeptide of 315 amino acids, demonstrating that Leuc. oenos 9204 HDC was synthesized as a precursor proHDC pi 6 (Mr 205,000). A cleavage between Ser-81 and Ser-82 generated the alpha (Mr 25,380) and beta (Mr 8840) chains, which suggested that the holoenzyme exists as a hexameric structure (alpha beta)6. At the optimal pH of 4.8, the HDC activity exhibited a simple Michaelis-Menten kinetic (K(m) = 0.33 mmol l-1, Vmax = 17.8 mumol CO2 min-1 mg-1), while at pH 7.6 it was sigmoidal (cooperativity index of 2). Histamine acted as a competitive inhibitor (Ki = 32 mmol l-1). The similarities of these results with those described for other bacterial HDC support the assumption that the pyruvoyl enzymes evolved from a common ancestral protein and have similar catalytic mechanisms. These results also confirmed that the main lactic acid bacterial species responsible for malolactic fermentation in red wine is able to produce histamine. Bacteria carrying the HDC activity must be avoided during selection of strains for the production of malolactic starters.
authors.affiliation: Laboratoire de Biotechnologie et Microbiologie Appliquée, Faculté d'Oenologie, Unité Associée Université de Bordeaux, Talence, France.
authors.forename: E
authors.initials: E
authors.lastname: Coton
authors.forename: G C
authors.initials: GC
authors.lastname: Rollan
authors.forename: A
authors.initials: A
authors.lastname: Lonvaud-Funel
mesh_terms: D000595:Amino Acid Sequence; D003001:Cloning, Molecular; D003016:Clostridium perfringens / Q000201:enzymology / Q000235:genetics; D005798:Genes, Bacterial; D006632:Histamine / Q000096:biosynthesis*; D006640:Histidine Decarboxylase / Q000235:genetics* / Q000302:isolation & purification / Q000378:metabolism; D007778:Lactobacillus / Q000201:enzymology / Q000235:genetics; D007934:Leuconostoc / Q000201:enzymology / Q000235:genetics*; D008837:Micrococcus / Q000201:enzymology / Q000235:genetics; D008969:Molecular Sequence Data; D011498:Protein Precursors / Q000235:genetics; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D013045:Species Specificity; D014920:Wine / Q000382:microbiology
chemical_list: D011498:Protein Precursors; D006632:Histamine; D006640:Histidine Decarboxylase