title: Cervical intraepithelial neoplasia and HPV infection.
abstract: Human papillomavirus (HPV) infection has been recognized as the major cause of cervical cancer [1]. The transformation zone of the cervix is the most frequent target of the high risk HPV types. In our study infection with HPV16 and 18 was investigated in the cervical scrapes of 28 subjects with cervical intraepithelial neoplasia. The DNAs isolated from the samples were amplified by dot-blot hybridization with fragmented digoxygenin labelled probes. Eighteen of 28 patients (64.3%) were found positive for HPV-16, eleven (39.3%) were found positive for HPV-18 and six patients (21%) were infected with both HPV types. This method is practical and sensitive for determining patients with a higher risk of developing HPV-related cancer.
authors.affiliation: Department of Obstetrics and Gynecology, Bucharest University Hospital, Romania.
authors.forename: P
authors.initials: P
authors.lastname: Virtej
authors.forename: M
authors.initials: M
authors.lastname: Matei
authors.forename: M
authors.initials: M
authors.lastname: Badea
authors.forename: I
authors.initials: I
authors.lastname: Badea
authors.forename: O
authors.initials: O
authors.lastname: Popa
mesh_terms: D000293:Adolescent; D000328:Adult; D003127:Colposcopy; D004279:DNA, Viral / Q000032:analysis*; D005260:Female; D006801:Humans; D017403:In Situ Hybridization; D015994:Incidence; D008875:Middle Aged; D027383:Papillomaviridae / Q000302:isolation & purification*; D030361:Papillomavirus Infections / Q000150:complications / Q000453:epidemiology / Q000473:pathology*; D016133:Polymerase Chain Reaction; D012307:Risk Factors; D012680:Sensitivity and Specificity; D014412:Tumor Virus Infections / Q000150:complications / Q000453:epidemiology / Q000473:pathology*; D002583:Uterine Cervical Neoplasms / Q000209:etiology / Q000473:pathology*; D014626:Vaginal Smears; D002578:Uterine Cervical Dysplasia / Q000209:etiology / Q000473:pathology*
chemical_list: D004279:DNA, Viral