Inducible cyclooxygenase expression in canine basilar artery after experimental subarachnoid hemorrhage.
OBJECTIVE
Inducible cyclooxygenase (COX-2) has been found to play a pathological role in cerebral insult. We investigated the expression of COX-2 in the basilar artery after experimental subarachnoid hemorrhage (SAH).


METHODS
In a canine "two-hemorrhage" model of SAH, the basilar arteries were obtained on day 2 after a cisternal injection of autologous blood or on days 4, 6, 7, or 9 after the second injection. Basilar arteries also were obtained 12 hours after intracisternal injection a cytokine: interleukin (IL)-1 beta (0.03 microgram), IL-6 (3 micrograms), or IL-8 (10 micrograms). Western blotting with a polyclonal anti-COX-2 antibody was performed in these arteries.


RESULTS
COX-2 protein was not demonstrated in the basilar artery in control animals without SAH. However, it was expressed in the basilar artery on days 2, 4, 6, and 7 after blood injection but not on day 9. Intracisternal injection of IL-1 beta, IL-6, or IL-8 also induced COX-2 in the basilar artery.


CONCLUSIONS
COX-2 expression was detected in basilar arterial tissue in both acute and chronic stages after SAH. Elevation of inflammatory cytokines after SAH may be involved in the induction of COX-2, which may produce sufficient quantities of eicosanoids to affect hemodynamics after SAH.
Osuka
K
K
Department of Neurosurgery, Nagoya University School of Medicine, Japan.
Suzuki
Y
Y
Watanabe
Y
Y
Takayasu
M
M
Yoshida
J
J
D000818:Animals; D001488:Basilar Artery / Q000201:enzymology* / Q000503:physiopathology*; D015153:Blotting, Western; D002533:Cerebral Angiography; D051546:Cyclooxygenase 2; D016207:Cytokines / Q000494:pharmacology; D004195:Disease Models, Animal; D004285:Dogs; D004790:Enzyme Induction / Q000187:drug effects; D005260:Female; D007276:Injections, Intraventricular; D007375:Interleukin-1 / Q000494:pharmacology; D015850:Interleukin-6 / Q000494:pharmacology; D016209:Interleukin-8 / Q000494:pharmacology; D007527:Isoenzymes / Q000032:analysis / Q000378:metabolism*; D008297:Male; D010544:Peroxidases / Q000032:analysis / Q000378:metabolism; D011451:Prostaglandin-Endoperoxide Synthases / Q000032:analysis / Q000378:metabolism*; D013345:Subarachnoid Hemorrhage / Q000378:metabolism*
D016207:Cytokines; D007375:Interleukin-1; D015850:Interleukin-6; D016209:Interleukin-8; D007527:Isoenzymes; D010544:Peroxidases; D051546:Cyclooxygenase 2; D011451:Prostaglandin-Endoperoxide Synthases