title: Development of agents for reinforcement of fluorescence on near-infrared ray excitation for immunohistological staining.
abstract: Fluorescence intensity of indocyanine green (ICG) derivative (ICG-sulfo-OSu) was too low for its use to detect microlesions. Therefore, we examined the effects of reinforcement agents on ICG-sulfo-OSu labeled antibodies. Solutions of distearoylphosphatic acid sodium salt (DSPA) and octylglucoside (OG) in physiological phosphate buffered saline (PBS) were found to increase the intensity of fluorescence of ICG-sulfo-OSu labeled antibodies, with shift in the fluorescence peak wavelength from 804 to 821 nm.
authors.affiliation: Second Department of Internal Medicine, School of Medicine, University of Tokushima, Japan. ito@clin.med.tokushima-u.ac.jp
authors.forename: S
authors.initials: S
authors.lastname: Ito
authors.forename: N
authors.initials: N
authors.lastname: Muguruma
authors.forename: S
authors.initials: S
authors.lastname: Hayashi
authors.forename: S
authors.initials: S
authors.lastname: Taoka
authors.forename: T
authors.initials: T
authors.lastname: Bando
authors.forename: K
authors.initials: K
authors.lastname: Inayama
authors.forename: M
authors.initials: M
authors.lastname: Sogabe
authors.forename: T
authors.initials: T
authors.lastname: Okahisa
authors.forename: S
authors.initials: S
authors.lastname: Okamura
authors.forename: H
authors.initials: H
authors.lastname: Shibata
authors.forename: T
authors.initials: T
authors.lastname: Irimura
authors.forename: K
authors.initials: K
authors.lastname: Takesako
authors.forename: S
authors.initials: S
authors.lastname: Shibamura
mesh_terms: D000906:Antibodies / Q000737:chemistry; D005455:Fluorescent Antibody Technique; D005456:Fluorescent Dyes / Q000592:standards*; D007208:Indocyanine Green / Q000031:analogs & derivatives* / Q000737:chemistry; D013055:Spectrophotometry, Infrared / Q000379:methods*
chemical_list: D000906:Antibodies; D005456:Fluorescent Dyes; C113792:indocyanine green-sulfo-OSu; D007208:Indocyanine Green