Integration host factor positively regulates cycJIH transcription.
We report the identification of integration host factor (IHF) as an additional element involved in the regulation of the cysJIH promoter of Salmonella typhimurium and Escherichia coli. An IHF-binding site was located in the regulatory region of the cysJIH promoter by using two methods, protection against DNase I digestion and hydroxyl radical cleavage. The positive influence of IHF on in vitro run-off transcription from the cysJIH promoter is shown. The in vivo observations suggest that IHF is necessary for full expression of cysJIH in stationary phase but not during exponential growth.
Sirko
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Institute of Biochemistry and Biophysics, Polish Academy of Sciences, Warsaw. asirko@ibbrain.ibb.waw.pl
Wegleńska
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Hulanicka
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D001426:Bacterial Proteins / Q000494:pharmacology*; D001483:Base Sequence; D001665:Binding Sites; D003545:Cysteine / Q000096:biosynthesis*; D004926:Escherichia coli / Q000235:genetics*; D005786:Gene Expression Regulation; D034982:Integration Host Factors; D008969:Molecular Sequence Data; D009154:Mutation; D011401:Promoter Regions, Genetic; D012486:Salmonella typhimurium / Q000235:genetics*
D001426:Bacterial Proteins; D034982:Integration Host Factors; C440508:integration host factor, Salmonella; D003545:Cysteine