Sex identification of elk (Cervus elaphus canadensis), moose (Alces alces), and white-tailed deer (Odocoileus virginianus) using the polymerase chain reaction.
We have developed a PCR-based protocol to determine the gender of tissue samples originating from elk (Cervus elaphus canadensis), moose (Alces alces) and white-tailed deer (Odocoileus virginianus). The technique simultaneously amplifies a conserved region of the sex-determining gene on the Y-chromosome (Sry) and a region of the Fragile X mental retardation gene (Fmr-1). The multiplex nature of this protocol allows the determination of gender using the Sry marker with the Fmr-1 marker providing an internal control. This technique is applicable to the enforcement of the validation tag system for game species. Data are provided from a wildlife investigation in Ontario.
Wilson
P J
PJ
Department of Biology, McMaster University, Hamilton, Ontario, Canada.
White
B N
BN
D000818:Animals; D001483:Base Sequence; D016172:DNA Fingerprinting / Q000662:veterinary*; D017931:DNA Primers / Q000737:chemistry; D004268:DNA-Binding Proteins / Q000235:genetics*; D003670:Deer / Q000235:genetics*; D004587:Electrophoresis, Agar Gel / Q000662:veterinary; D005260:Female; D051860:Fragile X Messenger Ribonucleoprotein 1; D005600:Fragile X Syndrome / Q000235:genetics; D005819:Genetic Markers; D008297:Male; D008969:Molecular Sequence Data; D009419:Nerve Tissue Proteins / Q000235:genetics*; D009687:Nuclear Proteins; D016133:Polymerase Chain Reaction / Q000662:veterinary*; D016601:RNA-Binding Proteins; D017422:Sequence Analysis, DNA / Q000662:veterinary; D012732:Sex Determination Analysis / Q000662:veterinary*; D051876:Sex-Determining Region Y Protein; D014157:Transcription Factors
D017931:DNA Primers; D004268:DNA-Binding Proteins; D051860:Fragile X Messenger Ribonucleoprotein 1; D005819:Genetic Markers; D009419:Nerve Tissue Proteins; D009687:Nuclear Proteins; D016601:RNA-Binding Proteins; D051876:Sex-Determining Region Y Protein; D014157:Transcription Factors