An antigenic protein gene of a phytoplasma associated with sweet potato witches' broom.
A gene encoding the major antigenic protein of phytoplasma associated with sweet potato witches' broom (SPWB) was cloned and analysed by screening the genomic library of SPWB phytoplasma with monoclonal antibodies for SPWB phytoplasma. The entire predicted structural gene encoded an antigenic protein composed of 172 amino acids with a computed molecular mass of 19.15 kDa and a pl value of 9.78. The -10 region of the promoter and the terminator region of the gene were identified and found to be similar to those of prokaryotes. The hydropathy profile of the deduced amino acid sequence consisted of two distinct regions, a strongly hydrophobic N-terminus and a highly hydrophilic C-terminus. This major antigenic protein was also present in phytoplasma associated with peanut witches' broom (PNWB) and the two showed homology based on the results of Western blot analysis, Southern hybridization, Northern hybridization, primer extension analysis and PCR. The homologous genes of the antigenic protein of SPWB phytoplasma and PNWB phytoplasma were not found in other phytoplasmas tested.
Yu
Yen-Ling
YL
Department of Plant Pathology and EntomologyTaiwan 106, Republic of China.
Yeh
Kai-Wun
KW
Department of Botany National Taiwan University, Taipei, Taiwan 106, Republic of China.
Lin
Chan-Pin
CP
Department of Plant Pathology and EntomologyTaiwan 106, Republic of China.
D000911:Antibodies, Monoclonal / Q000276:immunology; D000942:Antigens, Bacterial / Q000235:genetics* / Q000276:immunology; D010367:Arachis / Q000382:microbiology; D015152:Blotting, Northern; D015139:Blotting, Southern; D015153:Blotting, Western; D003001:Cloning, Molecular; D005798:Genes, Bacterial; D008969:Molecular Sequence Data; D009174:Mycoplasma / Q000235:genetics* / Q000276:immunology; D010935:Plant Diseases / Q000382:microbiology*; D016133:Polymerase Chain Reaction; D017422:Sequence Analysis, DNA; D019657:Solanaceae / Q000382:microbiology*
D000911:Antibodies, Monoclonal; D000942:Antigens, Bacterial