title: The biochip. A new membrane bioreactor system for the cultivation of animal cells in defined tissue-like cell densities.
abstract: Based on the laminar structure of the human liver tissue, a high cell density membrane bioreactor was developed that emulates a cell layer thickness of 40 microns. The "biochip" consists of a platinum-coated metal cell grid covered with two microfiltration membranes to form separate cell chambers of defined volume. Starting with a continuous chemostat process, the viability of a model suspension cell culture could be stabilized at 98%. In a second step these cells were transferred into the biochip system and were cultivated successfully for several days under tissue-like cell densities in a modified membrane holder under cross-flow conditions.
authors.affiliation: Institute of Cell Culture Technology, Faculty of Technical Sciences, University of Bielefeld, Germany. seewoet@BBC01.worcester.basf-corp.com
authors.forename: T
authors.initials: T
authors.lastname: Seewöster
authors.forename: S
authors.initials: S
authors.lastname: Wilmsmann
authors.forename: A
authors.initials: A
authors.lastname: Werner
authors.forename: J
authors.initials: J
authors.lastname: Lehmann
mesh_terms: D000818:Animals; D019149:Bioreactors; D016466:CHO Cells; D002452:Cell Count; D002478:Cells, Cultured; D006224:Cricetinae; D003584:Cytological Techniques; D006801:Humans; D008099:Liver / Q000166:cytology; D008567:Membranes, Artificial; D014462:Ultrafiltration
chemical_list: D008567:Membranes, Artificial