Staining of the midbody by an anti-digoxin-specific antibody.
Using RNA in situ hybridization to reveal cytoplasmic localization patterns of mRNAs in cultured cells, we noted unexpected staining of a cytoplasmic component in telophase cells. Control experiments revealed that the anti-digoxin-specific antibody was responsible for this staining. Because the staining was observed only at a position where both daughter cells are still connected, we identified the stained component as the midbody. This was confirmed by double staining of cells with anti-digoxin and anti-alpha-tubulin antibodies. We concluded that anti-digoxin-specific antibody shows crossreactivity with a component present in the midbody.
Dirks
R W
RW
Department of Molecular Cell Biology, Laboratory of Cytochemistry and Cytometry, Leiden University, Leiden, The Netherlands.
Raap
A K
AK
D000818:Animals; D000911:Antibodies, Monoclonal; D002478:Cells, Cultured; D003429:Cross Reactions; D004077:Digoxin / Q000276:immunology*; D006367:HeLa Cells; D006801:Humans; D007150:Immunohistochemistry; D017403:In Situ Hybridization; D051381:Rats; D008941:Spindle Apparatus / Q000276:immunology; D013692:Telophase / Q000276:immunology*; D014404:Tubulin / Q000276:immunology / Q000378:metabolism
D000911:Antibodies, Monoclonal; D014404:Tubulin; D004077:Digoxin