title: The surveillance complex interacts with the translation release factors to enhance termination and degrade aberrant mRNAs.
abstract: The nonsense-mediated mRNA decay pathway is an example of an evolutionarily conserved surveillance pathway that rids the cell of transcripts that contain nonsense mutations. The product of the UPF1 gene is a necessary component of the putative surveillance complex that recognizes and degrades aberrant mRNAs. Recent results indicate that the Upf1p also enhances translation termination at a nonsense codon. The results presented here demonstrate that the yeast and human forms of the Upf1p interact with both eukaryotic translation termination factors eRF1 and eRF3. Consistent with Upf1p interacting with the eRFs, the Upf1p is found in the prion-like aggregates that contain eRF1 and eRF3 observed in yeast [PSI+] strains. These results suggest that interaction of the Upf1p with the peptidyl release factors may be a key event in the assembly of the putative surveillance complex that enhances translation termination, monitors whether termination has occurred prematurely, and promotes degradation of aberrant transcripts.
authors.affiliation: Department of Molecular Genetics and Microbiology, Robert Wood Johnson Medical School-UMDNJ, USA.
authors.forename: K
authors.initials: K
authors.lastname: Czaplinski
authors.forename: M J
authors.initials: MJ
authors.lastname: Ruiz-Echevarria
authors.forename: S V
authors.initials: SV
authors.lastname: Paushkin
authors.forename: X
authors.initials: X
authors.lastname: Han
authors.forename: Y
authors.initials: Y
authors.lastname: Weng
authors.forename: H A
authors.initials: HA
authors.lastname: Perlick
authors.forename: H C
authors.initials: HC
authors.lastname: Dietz
authors.forename: M D
authors.initials: MD
authors.lastname: Ter-Avanesyan
authors.forename: S W
authors.initials: SW
authors.lastname: Peltz
mesh_terms: D005656:Fungal Proteins / Q000235:genetics* / Q000378:metabolism; D006801:Humans; D010454:Peptide Termination Factors / Q000235:genetics*; D014176:Protein Biosynthesis; D020365:RNA Helicases; D012333:RNA, Messenger / Q000235:genetics* / Q000378:metabolism; D012441:Saccharomyces cerevisiae; D029701:Saccharomyces cerevisiae Proteins; D015534:Trans-Activators; D014158:Transcription, Genetic
chemical_list: C489002:ETF1 protein, human; D005656:Fungal Proteins; D010454:Peptide Termination Factors; D012333:RNA, Messenger; D029701:Saccharomyces cerevisiae Proteins; D015534:Trans-Activators; C087971:peptide-chain-release factor 3; C074151:NAM7 protein, S cerevisiae; D020365:RNA Helicases; C101828:UPF1 protein, human