title: Flow cytometric DNA analyses of benign breast lesions detected by screening mammography.
abstract: There is little information regarding flow cytometric DNA analyses of benign breast lesions. This prospective study consists of mammographic and pathological correlation of DNA flow cytometric analyses of specimen mammography-guided fine-needle aspirates (FNAs) of 189 consecutive benign breast lesions and 114 FNAs of adjacent normal tissue as a control. Clinical follow-up was also performed. Aneuploidy was detected in 14 of 189 (7%) benign lesion specimen mammography-guided FNAs and in only 1 of 114 (0.9%) FNAs of adjacent normal tissue (P = 0.01). Aneuploidy was detected in two (33%) benign intramammary lymph nodes compared with four (12%) benign lesions with atypia, one benign lesion (3%) with hyperplasia, four benign lesions (10%) with adenosis, and three (4%) other benign lesions (P = 0.01). There were no significant associations between DNA content and S-phase percentage and patient age, mammographic appearance, or extent. During a median follow-up of 40 months (range, 6-84 months), 2 of 13 (15%) patients with aneuploid benign lesions developed ipsilateral breast carcinoma compared with 5 of 175 (3%) patients with diploid benign lesions (odds ratio, 6.18; 95% confidence interval, 1.08-35.56). Our data suggest that aneuploidy, which is detected in a variety of benign breast lesions, may be associated with a higher risk of development of breast carcinoma. The combined techniques of specimen mammography-guided fine-needle aspiration and flow cytometry provide a practical translational research method for the study of benign breast disease.
authors.affiliation: Division of Diagnostic Imaging, Roswell Park Cancer Institute, School of Medicine and Biomedical Sciences, State University of New York at Buffalo 14263, USA. stomper@sc3102.med.buffalo.edu
authors.forename: P C
authors.initials: PC
authors.lastname: Stomper
authors.forename: J R
authors.initials: JR
authors.lastname: DeBloom
authors.forename: R M
authors.initials: RM
authors.lastname: Budnick
authors.forename: C C
authors.initials: CC
authors.lastname: Stewart
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000782:Aneuploidy; D001707:Biopsy, Needle; D001940:Breast / Q000166:cytology / Q000473:pathology*; D001941:Breast Diseases / Q000000981:diagnostic imaging* / Q000473:pathology*; D001943:Breast Neoplasms / Q000517:prevention & control*; D004247:DNA / Q000032:analysis*; D004171:Diploidy; D005260:Female; D005434:Flow Cytometry / Q000379:methods; D006801:Humans; D006965:Hyperplasia; D008198:Lymph Nodes / Q000166:cytology / Q000473:pathology; D008327:Mammography; D008403:Mass Screening; D008875:Middle Aged; D016196:S Phase
chemical_list: D004247:DNA