[Determination and identification of beta-1,3;1,6-glycooligosaccharides in laminarioligosaccharide mixtures by means of enzymologic transglycosylation. Oligosaccharides with degree of polymerization of 5 and 6].
1-->3; 1-->6-beta-D-Glucooligosaccharides with polymerization degree of 5-6 were obtained from laminaran and analyzed by the previously developed method [1] by using them as donors and p-nitrophenyl beta-D-glucoside as the acceptor in the transglycosylation reaction catalysed by endo-1-->3-beta-D-glucanase LIV. The resulting homologous p-nitrophenyl beta-1,3-laminarioligosides with polymerization degree of 2-6 and the corresponding derivatives of mixed beta-1,3; 1,6-glucooligosaccharides with the same polymerization degree were analyzed by HPLC. The latter compounds exhibited higher retention times than the former with the same polymerization degree. Isomeric tetra-, penta-, and hexameric compounds were detected, and some of them were structurally characterized by means of NMR. The suggested method of analysis of oligosaccharide mixtures is simple, informative, and consumes a minimal quantity of sample.
Eliakova
L A
LA
Pacific Institute of Bioorganic Chemistry, Far East Division, Russian Academy of Sciences, Vladivostok, Russia.
Bakunina
I Iu
IIu
Mamontova
V A
VA
Isakov
V V
VV
D002240:Carbohydrate Sequence; D002851:Chromatography, High Pressure Liquid; D004693:Glucan Endo-1,3-beta-D-Glucosidase / Q000737:chemistry*; D005936:Glucans; D006031:Glycosylation; D006868:Hydrolysis; D007795:Laminaria / Q000737:chemistry; D009682:Magnetic Resonance Spectroscopy; D008969:Molecular Sequence Data; D009844:Oligosaccharides / Q000737:chemistry*; D011108:Polymers; D011134:Polysaccharides / Q000737:chemistry
D005936:Glucans; D009844:Oligosaccharides; D011108:Polymers; D011134:Polysaccharides; C008247:laminaran; D004693:Glucan Endo-1,3-beta-D-Glucosidase
Fermentativnoe transglikozilirovanie kak sposob obnaruzheniia i identifikatsii beta-1,3;1,6-gliukooligosakharidov v smesi laminarioligosakharidov. Oligosakharidy so stepen'iu polimerizatsii 5 i 6.