Mortality, size of the gonads, and ultrastructure of primordial germ cell in chick embryos treated with gamma-irradiation or injected with donor cells.
The effects of injection and/or gamma-irradiation prior to injection on mortality, size of the gonads, and ultrastructure of primordial germ cell (PGC) were examined after 5 d of incubation. The mortality of embryos injected with donor cells was significantly higher than that of control and irradiated embryos. All irradiated embryos were alive, although their development was delayed compared to those not exposed to irradiation. The size of the gonads of embryos injected with donor cells were similar to those of control embryos, however, the size of the gonads in irradiated embryos was significantly smaller than those of control embryos. The number of PGC in the gonads was significantly decreased by irradiation. There was no notable effect of irradiation or injection on the nuclei and cytoplasmic organelles in PGC.
Maeda
T
T
Faculty of Applied Biological Science, Hiroshima University, Higashi-Hiroshima, Japan. temaeda@ipc.hiroshima-u.ac.jp
Clark
M E
ME
Etches
R J
RJ
D000818:Animals; D001756:Blastoderm / Q000502:physiology*; D002470:Cell Survival / Q000502:physiology / Q000528:radiation effects; D002642:Chick Embryo / Q000166:cytology / Q000502:physiology* / Q000528:radiation effects; D003643:Death; D005260:Female; D005720:Gamma Rays; D005854:Germ Cells / Q000502:physiology* / Q000528:radiation effects / Q000648:ultrastructure; D008297:Male; D015044:Zona Pellucida / Q000637:transplantation*