Enhancing the survival of aspirated human fat injected into nude mice.
Injection of aspirated fat is now the most commonly used technique for the filling of depressed areas. Partial absorption of the injected fat is the main limitation of this procedure. Cariel T.M. is an enriched serum-free cell culture medium, its ability to enhance the survival of human aspirated fat grafts was investigated in the nude mouse model. A volume of 0.75-cc Cariel preprocessed fat was injected under the scalp skin of 16 nude mice in the experimental group, and the same volume of saline preprocessed fat was injected to 15 control group of mice. Significant maintenance of the weight, 46 percent in the experimental group compared with 29 percent in the control group (p < 0.008), and the volume, 44 percent in the experimental group compared with 31 percent in the control group (p < 0.026), was observed, after 15 weeks, in this newly used model. It seems that addition of the nutrients enriched with anabolic hormones enabled the survival and take of more adipose cells in the graft.
Ullmann
Y
Y
Department of Plastic and Reconstructive Surgery, Rambam Medical Center, Bruce Rappaport Faculty of Medicine, Technion IIT, Haifa, Israel.
Hyams
M
M
Ramon
Y
Y
Beach
D
D
Peled
I J
IJ
Lindenbaum
E S
ES
D000273:Adipose Tissue / Q000473:pathology / Q000637:transplantation*; D000596:Amino Acids; D000818:Animals; D003470:Culture Media; D016895:Culture Media, Serum-Free; D005947:Glucose; D006085:Graft Survival / Q000187:drug effects*; D006801:Humans; D007267:Injections; D008297:Male; D051379:Mice; D008819:Mice, Nude; D009930:Organic Chemicals; D012535:Scalp; D013396:Suction; D014183:Transplantation, Heterologous; D014815:Vitamins
D000596:Amino Acids; C112696:Cariel, culture medium; D003470:Culture Media; D016895:Culture Media, Serum-Free; D009930:Organic Chemicals; D014815:Vitamins; D005947:Glucose