Increased plasma P-selectin induced by intravenous administration of endotoxin in rats.
We developed a competitive enzyme immunoassay for detection of rat P-selectin and examined temporal changes of plasma P-selectin levels in an endotoxin-induced injury model in rats. Soluble P-selectin was detected in rat plasma after intravenous administration of lipopolysaccharide (LPS), and increased to a maximum level of five-fold over baseline after 24 hours. Plasma P-selectin was partially purified by gelfiltration and was identified as a 122 kDa band by Western blotting. Using the ELISA system we developed, monitoring of plasma P-selectin has become possible in the rat.
Misugi
E
E
Sumitomo Pharmaceuticals Research Center, Osaka, Japan.
Tojo
S J
SJ
Yasuda
T
T
Kurata
Y
Y
Morooka
S
S
D000818:Animals; D001667:Binding, Competitive; D004195:Disease Models, Animal; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods* / Q000592:standards; D007275:Injections, Intravenous; D007700:Kinetics; D008070:Lipopolysaccharides / Q000008:administration & dosage / Q000633:toxicity*; D008297:Male; D019007:P-Selectin / Q000096:biosynthesis / Q000097:blood*; D051381:Rats; D017207:Rats, Sprague-Dawley; D012015:Reference Standards; D012995:Solubility
D008070:Lipopolysaccharides; D019007:P-Selectin