The CP-I subunit of adenosine deaminase complexing protein from calf kidney is identical to human, mouse, and rat dipeptidyl peptidase IV.
The CP-I subunit of calf kidney adenosine deaminase complexing protein (ADCP), isolated by affinity chromatography based on Sepharose-4B immobilized adenosine deaminase, is identical with dipeptidyl peptidase IV. This finding is based on the following results: (a) Its M(r) = 110 kD, as determined by sodium dodecyl sulphate polyacrylamide gel electrophoresis; (b) its catalytic activity toward Gly-Pro-p-nitroanilide; (c) its inhibition by serine protease inhibitor; and (d) by two peptide sequences resulting from its trypsin proteolysis. Accordingly, the CP-I subunit of ADCP isolated from bovine kidney is DPPIV (CD26). Thus, as anticipated, the high affinity between ADA subunits prevails even when they originate in different species.
Ben-Shooshan
I
I
Department of Chemistry, Ben-Gurion University of the Negev, Beer-Sheva, Israel.
Parola
A H
AH
D000243:Adenosine Deaminase / Q000737:chemistry*; D000818:Animals; D002417:Cattle; D018819:Dipeptidyl Peptidase 4 / Q000032:analysis*; D006023:Glycoproteins / Q000737:chemistry*; D006801:Humans; D007527:Isoenzymes / Q000737:chemistry*; D007668:Kidney / Q000201:enzymology*; D051379:Mice; D010446:Peptide Fragments / Q000032:analysis*; D051381:Rats; D017421:Sequence Analysis; D017386:Sequence Homology, Amino Acid
D006023:Glycoproteins; D007527:Isoenzymes; D010446:Peptide Fragments; C042807:DPP4 protein, human; C000602659:DPP4 protein, rat; D018819:Dipeptidyl Peptidase 4; D000243:Adenosine Deaminase