FISH preimplantation diagnosis of chromosome aneuploidy in recurrent pregnancy wastage.
OBJECTIVE
Our purpose was to detect aneuploidy for chromosomes 13, 16, 18, 21, 22, X, and Y in preimplantation embryos from patients with a history of unexplained recurrent miscarriage.


METHODS
Three patients with a history of unexplained recurrent spontaneous abortion were included in this study. Embryos were biopsied at the eight-cell stage, individually fixed on slides, and processed for fluorescent in situ hybridization (FISH). A multiple FISH protocol for seven chromosomes pairs (13, 16, 18, 21, 22, X, and Y) has been developed.


RESULTS
A total of 39 embryos was studied with the multiple FISH protocol developed. Successful analysis of the biopsied embryos was achieved within the time limits usually allowed in a preimplantation diagnosis program. Analysis of the blastomeres showed that 17 embryos were chromosomally normal for the probes used, 16 embryos were aneuploid, and in 6 embryos no informative results were obtained.


CONCLUSIONS
In the patients studied, a large proportion of embryos (41%) exhibited chromosomal abnormalities for the probes used. Preimplantation diagnosis to screen for chromosome abnormalities could be a feasible approach to improve the possibility of successful pregnancy in these couples.
Vidal
F
F
Unitat de Biologia Cellular, Universitat Autònoma de Barcelona, Bellaterra, Spain.
Giménez
C
C
Rubio
C
C
Simón
C
C
Pellicer
A
A
Santaló
J
J
Egozcue
J
J
D000026:Abortion, Habitual / Q000235:genetics* / Q000473:pathology; D000328:Adult; D000782:Aneuploidy; D002869:Chromosome Aberrations / Q000175:diagnosis* / Q000235:genetics* / Q000473:pathology; D025063:Chromosome Disorders; D004622:Embryo, Mammalian / Q000473:pathology; D005260:Female; D006801:Humans; D017404:In Situ Hybridization, Fluorescence / Q000379:methods*; D011247:Pregnancy; D019836:Preimplantation Diagnosis / Q000379:methods*