Use of FLP/FRT system to study Drosophila development.
Marked clones in mosaic animals have been used extensively to answer developmental questions in Drosophila. Recently, the use of the FLP/FRT system has allowed for the high-frequency production of mosaic animals for 95% of Drosophila genes. Cell markers have been engineered in this system to label mutant clones in both developing tissues and adult cuticle. Strains carrying these genetically marked FLP/FRT chromosomes have greatly enhanced our ability to study gene function in both germline and somatic Drosophila cells.
Theodosiou
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Howard Hughes Medical Institute, Boyer Center for Molecular Medicine, Department of Genetics, Yale University School of Medicine, New Haven, Connecticut 06510, USA.
Xu
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D000818:Animals; D004254:DNA Nucleotidyltransferases / Q000235:genetics*; D004330:Drosophila / Q000196:embryology / Q000235:genetics* / Q000254:growth & development*; D017344:Genes, Insect / Q000235:genetics*; D005819:Genetic Markers / Q000235:genetics; D019476:Insect Proteins / Q000235:genetics*; D011995:Recombination, Genetic
D005819:Genetic Markers; D019476:Insect Proteins; D004254:DNA Nucleotidyltransferases; C045119:FLP recombinase