title: Perfusion quantitation in transplanted rat kidney by MRI with arterial spin labeling.
abstract: The purpose of this study was to determine the feasibility of using quantitative magnetic resonance imaging (MRI) with non-invasive arterial spin labeling to assess perfusion of transplanted kidneys in rats. MRI studies were performed on five groups of rats: normal Fisher 344 rats, Fisher 344 rats that had received a syngeneic kidney transplant either 3 or seven days prior to study, and Fisher 344 rats that had received an allogeneic kidney (ACI rat as the donor) either three or seven days prior to study. The contralateral native kidney remained in place for comparison. Cortical perfusion was quantitated from a slice through the center of each kidney in anesthetized rats at 4.7 Tesla with a fast gradient-echo MRI sequence following the arterial spin labeling. The spin-lattice relaxation time was measured within the cortex, and the cross sectional area of the kidney was also determined within the same MRI plane. Immediately after the perfusion imaging measurement, transplanted kidneys were removed and scored for rejection using the Banff histological criteria. Renal cortical perfusion in normal kidneys was 7.5 +/- 0.8 ml/g/min (N = 12 rats, 24 kidneys). At the third day post-transplantation, that is, before marked acute rejection, the renal cortical perfusion rate was similar in both syngeneic and allogeneic kidneys [3.3 +/- 1.7 (N = 6) and 3.0 +/- 2.4 ml/g/min (N = 6), respectively]. In contrast, at the seventh day post-transplantation, that is, during severe rejection, the renal cortical perfusion rate in allogeneic kidneys was very low (undetectable) compared to the value in syngeneic kidneys [that is, < or = 0.3 (N = 6) versus 5.2 +/- 2.0 ml/g/min (N = 6), respectively]. Moreover, the renal cortical perfusion rate determined by MRI was significantly (P < 0.05, r = -0.82) correlated with histological rejection. We conclude that the quantitative measurement of renal cortical perfusion by MRI with arterial spin-labeling could provide a non-invasive diagnostic method for monitoring the status of renal transplants without requiring the administration of a contrast agent.
authors.affiliation: Pittsburgh NMR Center for Biomedical Research, Carnegie Mellon University, Pennsylvania, USA.
authors.forename: J J
authors.initials: JJ
authors.lastname: Wang
authors.forename: K S
authors.initials: KS
authors.lastname: Hendrich
authors.forename: E K
authors.initials: EK
authors.lastname: Jackson
authors.forename: S T
authors.initials: ST
authors.lastname: Ildstad
authors.forename: D S
authors.initials: DS
authors.lastname: Williams
authors.forename: C
authors.initials: C
authors.lastname: Ho
mesh_terms: D000208:Acute Disease; D000818:Animals; D001158:Arteries; D005240:Feasibility Studies; D006084:Graft Rejection / Q000175:diagnosis / Q000473:pathology; D007668:Kidney / Q000473:pathology; D016030:Kidney Transplantation; D008279:Magnetic Resonance Imaging; D008297:Male; D051381:Rats; D011912:Rats, Inbred ACI; D011916:Rats, Inbred F344; D012079:Renal Circulation / Q000502:physiology; D013113:Spin Labels; D013997:Time Factors; D014184:Transplantation, Homologous; D014185:Transplantation, Isogeneic
chemical_list: D013113:Spin Labels