Activation of transcription factors and IL-8 expression in neutrophils stimulated with lipopolysaccharide from Porphyromonas gingivalis.
DNA binding activity of NF-kappa B and AP-1 were examined in neutrophils stimulated with LPS purified from P. gingivalis, a major pathogenic bacteria of periodontitis lesion. Porphyromonas gingivalis LPS enhanced the activity reaching a peak at a concentration of 500 ng/ml in the absence of serum. The NF-kappa B activation stimulated with 10 ng/ml of P. gingivalis LPS was suppressed approximately 44% by treatment of neutrophils with anti-CD14 antibody under the presence of serum. Increase in the steady-state IL-8 mRNA level was concomitantly observed by stimulation of neutrophils with 500 ng/ml of P. gingivalis LPS under the absence of serum. These results indicate that P. gingivalis LPS activates NF-kappa B and AP-1 in both serum-dependent and -independent manners, followed by increased IL-8 transcription in neutrophils, and suggested a role for P. gingivalis LPS in IL-8 synthesis by neutrophils in inflamed gingiva and GCF.
Sugita
N
N
Department of Periodontology, Niigata University School of Dentistry.
Kimura
A
A
Matsuki
Y
Y
Yamamoto
T
T
Yoshie
H
H
Hara
K
K
D000911:Antibodies, Monoclonal / Q000494:pharmacology; D004926:Escherichia coli; D006801:Humans; D017403:In Situ Hybridization; D016209:Interleukin-8 / Q000235:genetics / Q000378:metabolism*; D018950:Lipopolysaccharide Receptors / Q000276:immunology; D008070:Lipopolysaccharides / Q000494:pharmacology*; D016328:NF-kappa B / Q000502:physiology*; D009504:Neutrophils / Q000187:drug effects* / Q000378:metabolism*; D016966:Porphyromonas gingivalis; D012333:RNA, Messenger / Q000378:metabolism; D012260:Ribonucleases; D018808:Transcription Factor AP-1 / Q000502:physiology*
D000911:Antibodies, Monoclonal; D016209:Interleukin-8; D018950:Lipopolysaccharide Receptors; D008070:Lipopolysaccharides; D016328:NF-kappa B; D012333:RNA, Messenger; D018808:Transcription Factor AP-1; D012260:Ribonucleases