title: Poly[di(carboxylatophenoxy)phosphazene] (PCPP) is a potent immunoadjuvant for an influenza vaccine.
abstract: The adjuvant activity of poly[di(carboxylatophenoxy)phosphazene] (PCPP) on the immunogenicity of formalin-inactivated influenza virions and commercial trivalent influenza vaccine was studied. Regardless of which antigen preparation is used, the addition of 100 micrograms PCPP enhances the HAI antibody response 10-fold over the levels elicited by the vaccine alone. Similarly, PCPP enhanced the IgM, IgG, and IgG1 ELISA antibody titers to influenza antigens at least 10-fold higher than the vaccine alone. In contrast, the IgG2a isotype titers were only enhanced about 2-fold. Immunization of aged mice (22 months old) with trivalent influenza vaccine alone did not sero-convert these mice as measured by HAI or ELISA whereas significant sero-conversion was achieved when mice were immunized with PCPP-formulated trivalent vaccine. The adjuvant activity of PCPP was shown to not be due to a site of injection depot effect. PCPP adjuvanticity was positively correlated to the molecular weight of the polymer.
authors.affiliation: Virus Research Institute, Inc., Cambridge, MA 02138, USA. lpayne@VRII.com
authors.forename: L G
authors.initials: LG
authors.lastname: Payne
authors.forename: S A
authors.initials: SA
authors.lastname: Jenkins
authors.forename: A L
authors.initials: AL
authors.lastname: Woods
authors.forename: E M
authors.initials: EM
authors.lastname: Grund
authors.forename: W E
authors.initials: WE
authors.lastname: Geribo
authors.forename: J R
authors.initials: JR
authors.lastname: Loebelenz
authors.forename: A K
authors.initials: AK
authors.lastname: Andrianov
authors.forename: B E
authors.initials: BE
authors.lastname: Roberts
mesh_terms: D000276:Adjuvants, Immunologic / Q000737:chemistry; D000818:Animals; D000914:Antibodies, Viral / Q000276:immunology*; D005260:Female; D007252:Influenza Vaccines / Q000276:immunology*; D051379:Mice; D008807:Mice, Inbred BALB C; D008970:Molecular Weight; D009943:Organophosphorus Compounds / Q000737:chemistry; D009975:Orthomyxoviridae / Q000276:immunology*; D011108:Polymers / Q000737:chemistry; D014771:Virion / Q000276:immunology*
chemical_list: D000276:Adjuvants, Immunologic; D000914:Antibodies, Viral; C070410:Ca-poly(bis(carboxylatophenoxy)phosphazene); D007252:Influenza Vaccines; D009943:Organophosphorus Compounds; D011108:Polymers