Establishment and characterization of a new human testicular seminoma cell line, JKT-1.
BACKGROUND
A new human testicular cancer cell line (JKT-1) was established, successfully transplanted into nude mice, and has been maintained for over 2 years. We examined the biological characteristics of JKT-1 cells.


METHODS
The original material for JKT-1 was derived from a primary lesion of a left testicular seminoma (pure, typical-type) from a 40-year-old male. The tumor tissue was minced and cultured according to the explant culture method. The cells grew as a monolayer with a doubling time of 28.5 hours.


RESULTS
JKT-1 produced neither alpha-fetoprotein or beta-human chorionic gonadotropin, but showed strong immunoreactivities for vimentin and placental alkaline phosphatase. A chromosomal analysis revealed a modal number of 66 with loss of the Y chromosome. Morphologically, JKT-1 cells have a pleomorphic polygonal shape, an increase in the nuclear/cytoplasm ratio, and poor development of organelle and desmosome-like cell-cell junctions. JKT-1 cells were subcutaneously transplanted into the backs of 6-week-old nude mice, and grew classical seminomatous tissue.


CONCLUSIONS
This report profiled a seminoma cell line established for both in vitro and in vivo experimental systems. Future studies are planned to investigate germ cells using this seminoma line.
Kinugawa
K
K
Department of Urology, Kawasaki Medical School, Kurashiki, Japan.
Hyodo
F
F
Matsuki
T
T
Jo
Y
Y
Furukawa
Y
Y
Ueki
A
A
Tanaka
H
H
D000328:Adult; D000469:Alkaline Phosphatase / Q000378:metabolism; D000818:Animals; D002874:Chromosome Mapping; D006801:Humans; D007150:Immunohistochemistry / Q000379:methods; D008297:Male; D051379:Mice; D008819:Mice, Nude; D009368:Neoplasm Transplantation; D010920:Placenta / Q000201:enzymology; D018239:Seminoma / Q000235:genetics / Q000378:metabolism / Q000473:pathology*; D013194:Staining and Labeling; D013736:Testicular Neoplasms / Q000235:genetics / Q000378:metabolism / Q000473:pathology*; D014407:Tumor Cells, Cultured
D000469:Alkaline Phosphatase