title: Sodium dodecyl sulfate-agarose gel electrophoresis of urinary proteins: application to multiple myeloma.
abstract: We evaluated a new sodium dodecyl sulfate-agarose gel electrophoresis (SDS-AGE) for urinary protein analysis in patients with multiple myeloma (MM; n = 47; ages, 62 +/- 2 years, mean +/- SE). Abnormal proteinuria (mean = 1872 +/- 360 mg/24 h) was present in 95% of the samples; 75% of the patients had some sign of renal dysfunction (glomerular and/or tubular) according to their SDS-AGE pattern. A band suggesting Bence Jones proteinuria (BJP) was detected in 40 vs 33 specimens by routine AGE. Immunofixation identified BJP in 38 patients; the calculated sensitivity of SDS-AGE for BJP was 97%. Excellent correlation (P <0.0001) was obtained with routine AGE (r = 0.994) and immunonephelometry (r = 0.963) for light chain quantification. SDS-AGE allows easy evaluation of renal dysfunction and shows high sensitivity for BJP detection. In a specialized laboratory, it is useful for following the progress of MM patients through the semiquantification of BJP.
authors.affiliation: Laboratoire de Biochimie A, Hôpital St-Louis, Paris, France. Biochimie-a@chu-stlouis.fr
authors.forename: T
authors.initials: T
authors.lastname: Le Bricon
authors.forename: D
authors.initials: D
authors.lastname: Erlich
authors.forename: D
authors.initials: D
authors.lastname: Bengoufa
authors.forename: M
authors.initials: M
authors.lastname: Dussaucy
authors.forename: J P
authors.initials: JP
authors.lastname: Garnier
authors.forename: B
authors.initials: B
authors.lastname: Bousquet
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D001536:Bence Jones Protein / Q000652:urine*; D004587:Electrophoresis, Agar Gel / Q000379:methods; D005260:Female; D006801:Humans; D007122:Immunoelectrophoresis; D007147:Immunoglobulin Light Chains / Q000652:urine; D008297:Male; D008875:Middle Aged; D009101:Multiple Myeloma / Q000150:complications / Q000652:urine*; D009391:Nephelometry and Turbidimetry / Q000379:methods; D011507:Proteinuria / Q000150:complications / Q000652:urine*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D012967:Sodium Dodecyl Sulfate
chemical_list: D007147:Immunoglobulin Light Chains; D012967:Sodium Dodecyl Sulfate; D001536:Bence Jones Protein