title: Inhibition of human immunodeficiency virus type 1 virion entry by dominant-negative Hck.
abstract: To study the role of Src family tyrosine kinases in infection with human immunodeficiency virus type 1 (HIV-1), we constructed an Hck mutant, HckN, that hinders signaling from wild-type Hck. HIV-1 produced in HckN-expressing cells was significantly less infectious to HeLa-CD4-LTR-beta-gal (MAGI) cells than HIV-1 produced in mock-transfected cells. The inhibitory effect of HckN was compensated for by the expression of vesicular stomatitis virus G protein. Finally, we found that the HIV-1 produced in the HckN-expressing cells entered into the cells less efficiently than did the control HIV-1. These results suggest that the Src family tyrosine kinases regulate entry of HIV-1 into target cells.
authors.affiliation: Department of Pathology, National Institute of Infectious Diseases, Toyama, Shinjuku-ku, Tokyo 162-8640, Japan.
authors.forename: K
authors.initials: K
authors.lastname: Tokunaga
authors.forename: E
authors.initials: E
authors.lastname: Kiyokawa
authors.forename: M
authors.initials: M
authors.lastname: Nakaya
authors.forename: N
authors.initials: N
authors.lastname: Otsuka
authors.forename: A
authors.initials: A
authors.lastname: Kojima
authors.forename: T
authors.initials: T
authors.lastname: Kurata
authors.forename: M
authors.initials: M
authors.lastname: Matsuda
mesh_terms: D015693:Gene Products, nef / Q000502:physiology; D015497:HIV-1 / Q000502:physiology*; D006801:Humans; D009154:Mutation; D011505:Protein-Tyrosine Kinases / Q000235:genetics / Q000502:physiology*; D011518:Proto-Oncogene Proteins / Q000235:genetics / Q000502:physiology*; D051572:Proto-Oncogene Proteins c-hck; D015398:Signal Transduction; D014771:Virion / Q000502:physiology*; D054311:nef Gene Products, Human Immunodeficiency Virus
chemical_list: D015693:Gene Products, nef; D011518:Proto-Oncogene Proteins; D054311:nef Gene Products, Human Immunodeficiency Virus; D011505:Protein-Tyrosine Kinases; C496631:HCK protein, human; D051572:Proto-Oncogene Proteins c-hck