Regulation of cellular adhesion to extracellular matrix proteins by galectin-3.
The control of cellular adhesion to extracellular matrix proteins is poorly understood. In the present analyses, we set out to test the hypothesis that high galectin-3 concentration on the cell surface downregulates cellular adhesion to the extracellular matrix proteins. Various tumor cell lines were briefly incubated without or with galectin-3 and then allowed to adhere to wells coated with laminin-1, collagen IV and fibronectin. Our data demonstrated that the cells which were incubated with galectin-3 prior to plating had significantly reduced adhesion to extracellular matrix proteins. This inhibition involved the carbohydrate recognition domain of the lectin because adhesion was achieved in the presence of galectin-3 and lactose but not galectin-3 and sucrose. Furthermore we demonstrated that galectin-3 associates with alpha 1 beta 1 integrin in a lactose dependent manner.
Ochieng
J
J
Department of Biochemistry, Meharry Medical College, Nashville, Tennessee 37208, USA. ochien10@ccvax.mmc.edu
Leite-Browning
M L
ML
Warfield
P
P
D000818:Animals; D000943:Antigens, Differentiation / Q000494:pharmacology*; D002448:Cell Adhesion / Q000187:drug effects*; D003094:Collagen / Q000378:metabolism; D004305:Dose-Response Relationship, Drug; D016326:Extracellular Matrix Proteins / Q000378:metabolism*; D005260:Female; D005353:Fibronectins / Q000378:metabolism; D037502:Galectin 3; D006801:Humans; D039222:Integrin alpha1beta1; D016023:Integrins / Q000378:metabolism; D007797:Laminin / Q000378:metabolism; D008297:Male; D051379:Mice; D011485:Protein Binding; D014407:Tumor Cells, Cultured
D000943:Antigens, Differentiation; D016326:Extracellular Matrix Proteins; D005353:Fibronectins; D037502:Galectin 3; D039222:Integrin alpha1beta1; D016023:Integrins; D007797:Laminin; D003094:Collagen