title: Assay for etoposide in human serum using solid-phase extraction and high-performance liquid chromatography with fluorescence detection.
abstract: An HPLC assay for etoposide in human serum was developed. Serum, spiked with podophyllotoxin (internal standard), was treated with sodium dodecyl sulphate prior to solid phase extraction. Analysis was performed on a 300x3.9 mm Bondclone 10 C18 column coupled with a fluorometric detector (lambda(ex) 230 nm, lambda(em) 330 nm). The retention times for etoposide and podophyllotoxin were 14 and 28 min respectively. The range of assay was 0.5 to 20 microg/ml with a detection limit of 0.2 microg/ml. This assay is suitable for use in clinical studies with etoposide.
authors.affiliation: UNMC/Eppley Cancer Center Core Pharmacokinetics Laboratory, College of Pharmacy, University of Nebraska Medical Center, Omaha 68198-6025, USA.
authors.forename: K K
authors.initials: KK
authors.lastname: Manouilov
authors.forename: T R
authors.initials: TR
authors.lastname: McGuire
authors.forename: B G
authors.initials: BG
authors.lastname: Gordon
authors.forename: P R
authors.initials: PR
authors.lastname: Gwilt
mesh_terms: D000972:Antineoplastic Agents, Phytogenic / Q000097:blood*; D002851:Chromatography, High Pressure Liquid; D005047:Etoposide / Q000097:blood*; D006801:Humans; D007202:Indicators and Reagents; D011034:Podophyllotoxin; D012015:Reference Standards; D013050:Spectrometry, Fluorescence
chemical_list: D000972:Antineoplastic Agents, Phytogenic; D007202:Indicators and Reagents; D005047:Etoposide; D011034:Podophyllotoxin