Genotoxicity of the insecticide rotenone in cultured human lymphocytes.
We have investigated the genotoxic activity of rotenone on three genetic endpoints, sister-chromatid exchanges (SCE), chromosome aberrations (CA) and micronuclei (MN) in human lymphocyte cultures in the presence and absence of a metabolic activation system (S9 mix). Our results indicate that rotenone increases the frequency of binucleated micronucleated (BNMN) cells and causes a delay in the cell cycle but does not increase the frequency of CA and SCE at the concentrations used. The presence of S9 mix reduces the genotoxic activity of rotenone.
Guadaño
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A
Centro de Ciencias Medioambientales, CSIC, c/Serrano 115 dpdo, 28006 Madrid, Spain. aguadano@pinar1.csic.es
González-Coloma
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A
de la Peña
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E
D000818:Animals; D001711:Biotransformation; D002453:Cell Cycle / Q000187:drug effects; D002478:Cells, Cultured; D002869:Chromosome Aberrations; D006801:Humans; D007306:Insecticides / Q000633:toxicity*; D008214:Lymphocytes / Q000648:ultrastructure*; D008297:Male; D048629:Micronuclei, Chromosome-Defective / Q000187:drug effects; D008930:Mitochondria, Liver; D009153:Mutagens / Q000633:toxicity*; D051381:Rats; D017208:Rats, Wistar; D012402:Rotenone / Q000633:toxicity*; D012854:Sister Chromatid Exchange / Q000187:drug effects
D007306:Insecticides; D009153:Mutagens; D012402:Rotenone