title: Synovial chondromatosis of the temporomandibular joint: the effect of interleukin-1 on loose-body-derived cells.
abstract: OBJECTIVE
  The purpose of this study was to investigate the effect of interleukin-1 on cells from loose bodies of synovial chondromatosis of the temporomandibular joint.
  
  
  METHODS
  The cells were isolated from uncalcified loose bodies in a case of synovial chondromatosis of the temporomandibular joint and cultured in alpha-MEM medium containing 10% fetal bovine serum. The cells were treated with or without interleukin-1alpha and then stained with toluidine blue. Their conditioned media were analyzed with gelatin zymography to detect matrix-degrading proteinase(s).
  
  
  RESULTS
  The cells from loose bodies produced toluidine-blue-stained matrix. When the cells were treated with 100 ng/ml of interleukin-1alpha for 3 days, toluidine-blue-stained matrix was strikingly reduced. Gelatin zymography revealed that interleukin-1alpha-treated cells released 62-kDa gelatinase.
  
  
  CONCLUSIONS
  Interleukin-1alpha may lead loose-body-derived cells to degrade the cartilaginous matrix of loose bodies in synovial chondromatosis of the temporomandibular joint.
authors.affiliation: Second Department of Oral and Maxillofacial Surgery, Kyushu University School of Dentistry, Fukuoka, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Ikebe
authors.forename: E
authors.initials: E
authors.lastname: Nakayama
authors.forename: M
authors.initials: M
authors.lastname: Shinohara
authors.forename: H
authors.initials: H
authors.lastname: Takeuchi
authors.forename: Y
authors.initials: Y
authors.lastname: Takenoshita
mesh_terms: D000818:Animals; D002356:Cartilage / Q000378:metabolism / Q000473:pathology; D002417:Cattle; D002478:Cells, Cultured; D019902:Chondrocytes / Q000378:metabolism / Q000473:pathology; D015838:Chondromatosis, Synovial / Q000201:enzymology / Q000473:pathology*; D004396:Coloring Agents; D003470:Culture Media; D017077:Culture Media, Conditioned / Q000032:analysis; D010450:Endopeptidases / Q000032:analysis; D005109:Extracellular Matrix / Q000378:metabolism; D018093:Gelatinases / Q000032:analysis; D006801:Humans; D007375:Interleukin-1 / Q000494:pharmacology*; D007594:Joint Loose Bodies / Q000201:enzymology / Q000473:pathology*; D008297:Male; D008875:Middle Aged; D013705:Temporomandibular Joint Disorders / Q000201:enzymology / Q000473:pathology*; D014048:Tolonium Chloride
chemical_list: D004396:Coloring Agents; D003470:Culture Media; D017077:Culture Media, Conditioned; D007375:Interleukin-1; D014048:Tolonium Chloride; D010450:Endopeptidases; D018093:Gelatinases