Muscarinic receptor subtypes in porcine detrusor: comparison with humans and regulation by bladder augmentation.
The properties of muscarinic acetylcholine receptors of porcine and human bladder detrusor were compared in radioligand binding studies using [3H]quinuclidinylbenzylate as the radioligand. The receptor affinity for the radioligand and the density of muscarinic receptors was similar in male and female pigs and in humans (Kd = 35 +/- 8 pM, Bmax = 153 +/- 30 fmol/mg protein). Atropine and subtype-selective antagonists had steep and monophasic competition curves in porcine and human detrusor with a rank order of potency of atropine >> hexahydro-sila-difenidol > or = AF-DX 116 > or = pirenzepine, indicating the presence of a homogeneous population of M2 muscarinic receptors. In female pigs bladder outflow obstruction generated by partial urethral ligation or its surgical treatment by ileum augmentation or autoaugmentation did not significantly alter expression of muscarinic receptors or of alpha2A-adrenoceptors, but the power was insufficient to exclude alterations of less than 60%. We conclude that porcine and human detrusor express muscarinic receptors of the M2 subtype; despite these qualitative similarities the use of the porcine model may be limited by large biological variance with regard to quantitative receptor expression.
Goepel
M
M
Department of Urology, University of Essen Medical School, Germany.
Gronewald
A
A
Krege
S
S
Michel
M C
MC
D000818:Animals; D001667:Binding, Competitive; D005260:Female; D006801:Humans; D066298:In Vitro Techniques; D007700:Kinetics; D008297:Male; D008954:Models, Biological; D018727:Muscarinic Antagonists / Q000378:metabolism; D009130:Muscle, Smooth / Q000378:metabolism*; D019832:N-Methylscopolamine / Q000378:metabolism; D011813:Quinuclidinyl Benzilate / Q000378:metabolism; D011869:Radioligand Assay; D018341:Receptors, Adrenergic, alpha-2 / Q000378:metabolism; D011976:Receptors, Muscarinic / Q000145:classification* / Q000378:metabolism; D013045:Species Specificity; D013552:Swine; D001743:Urinary Bladder / Q000378:metabolism*; D001748:Urinary Bladder Neck Obstruction / Q000378:metabolism / Q000601:surgery
D018727:Muscarinic Antagonists; D018341:Receptors, Adrenergic, alpha-2; D011976:Receptors, Muscarinic; D011813:Quinuclidinyl Benzilate; D019832:N-Methylscopolamine