title: BALB/c 3T3 cells co-expressing FGF-2 and soluble FGF receptor acquire tumorigenicity.
abstract: The physiological significance of the soluble fibroblast growth factor (FGF) receptors is not clear yet although they are present in blood, vitreous fluid and in the extracellular matrix of vascular endothelial cells. A hypothesis that they might help FGF-2 release from cells is very interesting because FGF-2 does not have clear secretion signal and the mechanism of the secretion of FGF-2 is still unclear. Single overexpression of FGF-2 is related neither to the secretion potential of the molecule nor to the tumorigenicity of the cells. In this report, BALB/c 3T3 cells transformed with the full length of human FGF-2 cDNA are further transformed with the cDNA coding the extracellular domain of human FGF receptor 1. The obtained transformants co-expressing FGF-2 and soluble FGF receptor are highly tumorigenic in nude mice, while the parental cells do not show any tumorigenicity. In the conditioned medium of the double-transformants, FGF-2 is immunologically detected. These results suggest that naturally produced soluble form of FGF receptor supports the release of FGF-2 from the cells and that over-expression of these two molecules leads to induce the malignant tumours in vivo.
authors.affiliation: Department of Bioscience and Biotechnology, Faculty of Engineering, Okayama University, Japan. senom@biotech.okayama-u.ac.jp
authors.forename: M
authors.initials: M
authors.lastname: Seno
authors.forename: A
authors.initials: A
authors.lastname: Masago
authors.forename: A
authors.initials: A
authors.lastname: Nishimura
authors.forename: H
authors.initials: H
authors.lastname: Tada
authors.forename: M
authors.initials: M
authors.lastname: Kosaka
authors.forename: R
authors.initials: R
authors.lastname: Sasada
authors.forename: K
authors.initials: K
authors.lastname: Igarashi
authors.forename: S
authors.initials: S
authors.lastname: Seno
authors.forename: H
authors.initials: H
authors.lastname: Yamada
mesh_terms: D016475:3T3 Cells; D000818:Animals; D015197:Carcinogenicity Tests; D016222:Fibroblast Growth Factor 2 / Q000096:biosynthesis / Q000235:genetics / Q000502:physiology*; D015870:Gene Expression; D006801:Humans; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D017468:Receptors, Fibroblast Growth Factor / Q000096:biosynthesis / Q000235:genetics / Q000502:physiology*; D012995:Solubility
chemical_list: D017468:Receptors, Fibroblast Growth Factor; D016222:Fibroblast Growth Factor 2