title: A minimal avian retroviral packaging sequence has a complex structure.
abstract: We have defined a 160-nucleotide region, Mpsi, from the 5' leader region of the Rous sarcoma virus genome that is sufficient to direct the packaging of a heterologous RNA. Mpsi contains the putative O3 stem structure that has previously been shown, and that has been confirmed in this study, to be important for the efficient packaging of avian leukosis-sarcoma virus RNA. Analyses of several O3 stem mutants revealed that other regions within Mpsi can interfere with the proper folding of altered sequences which are predicted to form a wild-type O3 stem.
authors.affiliation: Division of Basic Sciences, Fred Hutchinson Cancer Research Center, Seattle, Washington 98109, USA.
authors.forename: J D
authors.initials: JD
authors.lastname: Banks
authors.forename: A
authors.initials: A
authors.lastname: Yeo
authors.forename: K
authors.initials: K
authors.lastname: Green
authors.forename: F
authors.initials: F
authors.lastname: Cepeda
authors.forename: M L
authors.initials: ML
authors.lastname: Linial
mesh_terms: D001358:Avian Sarcoma Viruses / Q000502:physiology*; D001483:Base Sequence; D008969:Molecular Sequence Data; D012367:RNA, Viral / Q000737:chemistry*; D019065:Virus Assembly
chemical_list: D012367:RNA, Viral