Diagnosis of sickle-cell disease by DNA analysis in Nigeria.
OBJECTIVE
To compare genotyping of leucocyte DNA by PCR and Dde I with the conventional genotyping of haemoglobin of the same subject.


METHODS
Comparative study.


METHODS
25 adults, 16 males and nine females.


METHODS
Predictability.


RESULTS
In all cases the results were comparable.


CONCLUSIONS
The technology can be applied locally to prenatal diagnosis of sickle-cell disease by chorionic sampling and, therefore, improve the management of sickle-cell disease in Nigeria.
Adewole
T A
TA
Nigerian Institute of Medical Research, Yaba-Lagos, Nigeria.
Olukoya
D K
DK
Afonja
A
A
D000328:Adult; D000755:Anemia, Sickle Cell / Q000175:diagnosis* / Q000235:genetics*; D016022:Case-Control Studies; D015193:Chorionic Villi Sampling; D004247:DNA / Q000032:analysis*; D004588:Electrophoresis, Cellulose Acetate; D005260:Female; D005838:Genotype; D006801:Humans; D007962:Leukocytes; D008297:Male; D009549:Nigeria; D016133:Polymerase Chain Reaction / Q000379:methods*; D015203:Reproducibility of Results; D015183:Restriction Mapping / Q000379:methods*
D004247:DNA