A glycine-rich RNA-binding protein gene is differentially expressed during acute hypersensitive response following Tobacco Mosaic Virus infection in tobacco.
During efforts for cloning disease resistance-responsive genes, a cDNA encoding a putative Nicotiana glutinosa glycine-rich RNA binding protein (ngRBP) was isolated from TMV induced cDNA library. Northern blot hybridization revealed that ngRBP gene is negatively regulated during early hours of TMV induced acute hypersensitive response (HR). Under greenhouse conditions induced expression of ngRBP gene was observed after 24 h following TMV infection. Salicylic acid and copper also induced ngRBP mRNA expression. Our findings are suggestive of some possible role for ngRBP in plant-pathogen interaction.
Naqvi
S M
SM
Plant Protectants Research Unit, Korea Research Institute of Bioscience and Biotechnology, Yusung, Taejon, South Korea.
Park
K S
KS
Yi
S Y
SY
Lee
H W
HW
Bok
S H
SH
Choi
D
D
D000595:Amino Acid Sequence; D003300:Copper / Q000494:pharmacology; D018076:DNA, Complementary; D018506:Gene Expression Regulation, Plant / Q000187:drug effects; D015967:Gene Expression Regulation, Viral / Q000187:drug effects; D008969:Molecular Sequence Data; D010935:Plant Diseases / Q000235:genetics*; D010940:Plant Proteins / Q000235:genetics*; D010947:Plants, Toxic; D016601:RNA-Binding Proteins / Q000235:genetics*; D012459:Salicylates / Q000494:pharmacology; D020156:Salicylic Acid; D017386:Sequence Homology, Amino Acid; D014026:Nicotiana / Q000235:genetics* / Q000382:microbiology; D014027:Tobacco Mosaic Virus / Q000472:pathogenicity*
D018076:DNA, Complementary; D010940:Plant Proteins; D016601:RNA-Binding Proteins; D012459:Salicylates; C100791:glycine-rich RNA-binding protein, plant; D003300:Copper; D020156:Salicylic Acid