Detection of MYC gene amplification in malignant lymphomas.
The role of oncogene or tumor supressor gene in Epstein-Barr virus (EBV) associated malignant lymphomas (MLs) is poorly understood. We examined 36 MLs (21 EBV positive and 15 EBV negative) and 6 reactive hyperplasias for the presence of myc gene amplification. Polymerase chain reaction (PCR) technology was used to examine the state of amplification of the proto-oncogene c-myc in formalin-fixed paraffin-embedded tissues. Variable degrees of myc gene amplification were detected in reactive hyperplasias and MLs. However, significant increase of c-myc copy numbers above 3 times were only found in 12 out of 31 non-Hodgkin's MLs (38.7%), in which 6 cases were EBV positive and 6 cases were EBV negative. In conclusion, myc gene amplification appears to play a part in MLs but no correlation was found between EBV infection and myc gene amplification.
Park
C K
CK
Department of Pathology, Hanyang University, College of Medicine, Seoul, Korea.
Lee
C G
CG
Lee
J D
JD
D005871:Castleman Disease / Q000235:genetics; D004279:DNA, Viral / Q000032:analysis; D005784:Gene Amplification; D004854:Herpesvirus 4, Human / Q000235:genetics / Q000302:isolation & purification; D006689:Hodgkin Disease / Q000235:genetics* / Q000821:virology; D006801:Humans; D006965:Hyperplasia / Q000235:genetics; D008228:Lymphoma, Non-Hodgkin / Q000235:genetics* / Q000821:virology; D016133:Polymerase Chain Reaction; D000090063:Proto-Oncogene Mas; D016271:Proto-Oncogene Proteins c-myc / Q000235:genetics*
D004279:DNA, Viral; C000715470:MAS1 protein, human; D000090063:Proto-Oncogene Mas; D016271:Proto-Oncogene Proteins c-myc