Analysis of methaqualone in biological matrices by micellar electrokinetic capillary chromatography. Comparison with gas chromatography-mass spectrometry.
The analysis of methaqualone (MTQ) in biological matrices by capillary electrophoresis (CE) is described. This methods uses liquid-liquid extraction and micellar electrokinetic capillary chromatography (MECC), an operation mode of CE. Separations are made using a 25 cm long capillary and a borate/phosphate buffer at pH 8.2. Using gas chromatography with mass spectrometry detection (GC-MS) as reference method, MTQ has been analyzed in urine, blood, gastric content and hair. For hair analysis, supercritical fluid extraction was compared with liquid-liquid extraction. Linearity was established in urine and blood between 0.25 and 10.0 micrograms/ml. MTQ recovery from blood was estimated at 60%. The limit of detection of this method in urine is about 0.10 microgram/ml. Drawbacks and advantages of MECC over GC-MS are discussed.
Plaut
O
O
Institute of Forensic Medicine, University of Geneva, Switzerland.
Girod
C
C
Staub
C
C
D001774:Blood Chemical Analysis; D019075:Electrophoresis, Capillary / Q000379:methods; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods; D005766:Gastrointestinal Contents / Q000737:chemistry; D006197:Hair / Q000737:chemistry; D006801:Humans; D006993:Hypnotics and Sedatives / Q000032:analysis*; D008702:Methaqualone / Q000032:analysis*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D014556:Urine / Q000737:chemistry
D006993:Hypnotics and Sedatives; D008702:Methaqualone