An experimental selection system to identify bacterial cells exhibiting a new DNA host specificity.
Restriction-modification enzymes interact with DNA sequences in a highly specific manner. Mutations within the DNA binding region of the enzymes could be expected to produce enzyme variants with changed DNA sequence specificities. We developed an efficient in vivo selection system that enabled us to detect one cell coding for a restriction-modification system with a new DNA sequence specificity in a background of more than 10(6) cells with the original DNA sequence specificity.
Kunz
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A
Institute of Medical Virology, Charité Medical School, Humboldt University, Berlin, Germany.
Meisel
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A
Mackeldanz
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P
Reuter
M
M
Krüger
D H
DH
D010582:Bacteriophage lambda / Q000235:genetics; D004279:DNA, Viral / Q000378:metabolism*; D015263:Deoxyribonucleases, Type III Site-Specific / Q000235:genetics / Q000378:metabolism*; D004926:Escherichia coli / Q000201:enzymology*; D008780:Methyltransferases / Q000235:genetics / Q000378:metabolism*
D004279:DNA, Viral; D008780:Methyltransferases; C030432:endodeoxyribonuclease EcoP1; C023451:endodeoxyribonuclease EcoP15I; D015263:Deoxyribonucleases, Type III Site-Specific