title: Combined use of analytical high-performance liquid chromatography and cell morphology transformation assay to detect new protein phosphatase inhibitors of okadaic acid type.
abstract: The search for new protein phosphatase inhibitors in shellfish contaminated by toxin-producing dinoflagellates generally relies on preliminary separation techniques followed by biological tests. To detect such substances without purifying them initially, we developed an approach based on a correlation of the results of two different analytical techniques applied to toxic extracts: high-performance liquid chromatography after derivation of the toxins and the cell morphology transformation assay on KB cells. Application of this protocol to stored frozen mussels showed a decrease in okadaic acid concentration during storage, with formation of degradation derivatives, some of which possessed notable protein phosphatase inhibition activity.
authors.affiliation: Université de Nantes, SMAB-URM11, ISOMER, Faculté de Pharmacie, France.
authors.forename: Y F
authors.initials: YF
authors.lastname: Pouchus
authors.forename: S
authors.initials: S
authors.lastname: Dauvergne
authors.forename: D
authors.initials: D
authors.lastname: Morel
authors.forename: F
authors.initials: F
authors.lastname: Mondeguer
authors.forename: C
authors.initials: C
authors.lastname: Marcaillou-Lebaut
authors.forename: Z
authors.initials: Z
authors.lastname: Amzil
authors.forename: J F
authors.initials: JF
authors.lastname: Verbist
mesh_terms: D000818:Animals; D049872:Bivalvia; D002478:Cells, Cultured; D002851:Chromatography, High Pressure Liquid; D004791:Enzyme Inhibitors / Q000302:isolation & purification*; D008387:Marine Toxins / Q000302:isolation & purification*; D019319:Okadaic Acid / Q000302:isolation & purification*; D010749:Phosphoprotein Phosphatases / Q000037:antagonists & inhibitors*
chemical_list: D004791:Enzyme Inhibitors; D008387:Marine Toxins; D019319:Okadaic Acid; D010749:Phosphoprotein Phosphatases