title: Cyclin D1 and Cyclin E expression in malignant thyroid cells and in human thyroid carcinomas.
abstract: Evidence of the involvement of cyclin gene alterations in human cancer is growing. In this study, we sought to determine the pattern of expression of cyclin D1 and cyclin E in normal and malignant thyroid cells. Quiescent rat thyroid cells in culture, induced to synthesize DNA by thyrotropin (TSH), expressed cyclin D1 gene after 6 hr and cyclin E gene with a peak at 18 hr from the stimulus; K-ras-transformed rat thyroid cells, which grew without addition of hormones necessary for normal cell proliferation, expressed elevated levels of cyclin D1 and cyclin E, compared with normal differentiated thyroid cells. Human benign and malignant thyroid tumors and their relative normal tissues were then analyzed. Neither major genetic alterations nor amplifications for cyclin D1 and cyclin E genes were found by Southern blot analysis in genomic DNAs extracted from all types of thyroid tumors. Moreover, statistical analyses of densitometric values from Northern blots did not show increased levels of cyclin D1 and E mRNAs in the tumor samples, compared with normal thyroid. Immunohistochemical analyses of formalin-fixed paraffin-embedded sections of tissues with specific antibodies revealed a prevalent cytoplasmic cyclin E staining in the thyroid tissues analyzed. Cyclin D1, instead, was present in the cytoplasm of normal thyroids and adenomas, but in 31% of thyroid papillary carcinomas analysed, it was overexpressed, with a localization in the nucleus. Our in vivo observations suggest that unlike cyclin E, elevated nuclear cyclin D1 expression defines a subset of thyroid papillary carcinomas, and might be a contributory factor to thyroid tumorigenesis.
authors.affiliation: Dipartimento di Medicina Sperimentale e Patologia, Università "La Sapienza," Rome, Italy.
authors.forename: D
authors.initials: D
authors.lastname: Lazzereschi
authors.forename: L
authors.initials: L
authors.lastname: Sambuco
authors.forename: C
authors.initials: C
authors.lastname: Carnovale Scalzo
authors.forename: A
authors.initials: A
authors.lastname: Ranieri
authors.forename: G
authors.initials: G
authors.lastname: Mincione
authors.forename: F
authors.initials: F
authors.lastname: Nardi
authors.forename: G
authors.initials: G
authors.lastname: Colletta
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D019938:Cyclin D1 / Q000032:analysis / Q000235:genetics*; D019927:Cyclin E / Q000032:analysis / Q000235:genetics*; D005786:Gene Expression Regulation; D006801:Humans; D007150:Immunohistochemistry; D012333:RNA, Messenger / Q000032:analysis; D051381:Rats; D013961:Thyroid Gland / Q000378:metabolism*; D013964:Thyroid Neoplasms / Q000378:metabolism*; D013972:Thyrotropin / Q000494:pharmacology
chemical_list: D019927:Cyclin E; D012333:RNA, Messenger; D019938:Cyclin D1; D013972:Thyrotropin