title: Suppression of somatosensory evoked potentials by nitric oxide synthase inhibition in rats: methodological differences.
abstract: We have previously shown that topically applied N(G)-nitro-L-arginine (L-NNA), a nitric oxide synthase (NOS) inhibitor, suppressed both somatosensory evoked potentials (SEPs) and vascular responses during sciatic nerve stimulation in rats. Due to the normal tight coupling between cerebral blood flow and neuronal activity, we surmise that the vascular response attenuation may be secondary to the SEP decrease. However, a recent study, in which SEPs were recorded with a 'non-contact' electrode placed longitudinally across the cranial window without touching the cortex, did not find a SEP decrease following NOS inhibition. In the present study, we compared SEPs recorded with 'contact' and 'non-contact' electrodes. Regardless of stimulation methods (sciatic nerve or hindpaw), an electrode in contact with the pial surface overlying the hindlimb somatosensory cortex recorded a steady SEP decline during I-NNA application. In contrast, a 'non-contact' electrode did not detect a significant SEP change in the presence of I-NNA. The present results thus confirm the attenuation of SEPs by NOS inhibition.
authors.affiliation: Department of Neurological Surgery, University of Washington School of Medicine, Harborview Medical Center, Seattle 98104-2499, USA. ngai@u.washington.edu
authors.forename: A C
authors.initials: AC
authors.lastname: Ngai
authors.forename: J R
authors.initials: JR
authors.lastname: Meno
authors.forename: M A
authors.initials: MA
authors.lastname: Jolley
authors.forename: H R
authors.initials: HR
authors.lastname: Winn
mesh_terms: D000818:Animals; D002560:Cerebrovascular Circulation / Q000502:physiology; D005073:Evoked Potentials, Somatosensory / Q000187:drug effects / Q000502:physiology*; D008297:Male; D019001:Nitric Oxide Synthase / Q000037:antagonists & inhibitors*; D019335:Nitroarginine / Q000494:pharmacology; D051381:Rats; D017207:Rats, Sprague-Dawley; D012584:Sciatic Nerve / Q000502:physiology; D013003:Somatosensory Cortex / Q000098:blood supply / Q000201:enzymology*
chemical_list: D019335:Nitroarginine; D019001:Nitric Oxide Synthase