title: A gene, yaeQ, that suppresses reduced operon expression caused by mutations in the transcription elongation gene rfaH in Escherichia coli and Salmonella typhimurium.
abstract: RfaH is a specialised transcription elongation protein. We isolated from a Salmonella typhimurium rfaH suppressor strain a gene that complements in trans the attenuated transcription of the hlyCABD hemolysin operon caused by an rfaH mutation. The gene is homologous to the uncharacterised Escherichia coli gene yaeQ. YaeQ did not increase hlyCABD-encoded hemolysin activity in the RfaH+ wild type, nor did it increase the level of RfaH protein. YaeQ also complements a rfaH::Tn5 null mutation, indicating that it compensates for loss of RfaH function without interaction between the two proteins.
authors.affiliation: Department of Pathology, University of Cambridge, UK.
authors.forename: K R
authors.initials: KR
authors.lastname: Wong
authors.forename: C
authors.initials: C
authors.lastname: Hughes
authors.forename: V
authors.initials: V
authors.lastname: Koronakis
mesh_terms: D001426:Bacterial Proteins / Q000235:genetics*; D004926:Escherichia coli / Q000235:genetics*; D029968:Escherichia coli Proteins; D015964:Gene Expression Regulation, Bacterial; D005798:Genes, Bacterial / Q000235:genetics*; D016153:Genes, Suppressor / Q000235:genetics*; D006460:Hemolysin Proteins / Q000235:genetics; D009154:Mutation / Q000235:genetics; D009876:Operon / Q000235:genetics; D010445:Peptide Elongation Factors / Q000235:genetics*; D012486:Salmonella typhimurium / Q000235:genetics*; D015534:Trans-Activators / Q000235:genetics*; D014158:Transcription, Genetic
chemical_list: D001426:Bacterial Proteins; D029968:Escherichia coli Proteins; D006460:Hemolysin Proteins; D010445:Peptide Elongation Factors; C106401:RfaH protein, E coli; D015534:Trans-Activators; C112339:YaeQ protein, Salmonella typhimurium