title: Imaging experimental osteomyelitis using radiolabeled liposomes.
abstract: We evaluated radiolabeled liposomes (liposomes labeled both with 99mTc and 111In) for the early detection of osteomyelitis in an experimental model.
  
  
  METHODS
  Liposomes, containing 5% polyethylene glycol-distearoyl phosphatidylethanolamine with encapsulated glutathione and deferoxamine, were prepared and labeled with 99mTc and 111In by a previously described method. Acute osteomyelitis was induced in male New Zealand rabbits by intramedullary injection of sodium-morrhuate and Staphylococcus aureus in the tibial bone marrow. Serial imaging studies, consisting of radiolabeled liposome imaging (2-4 mCi 99mTc and 75-125 microCi 111In), 99mTc-methylene diphosphonate (MDP) (3-5 mCi) and 67Ga-citrate (500 microCi), were performed starting at the third day after injection. Each radionuclide study was separated by at least 2 days. The animals also underwent radiography of the lower extremities. The animals were then killed and the infected tibia was excised for histopathology.
  
  
  RESULTS
  For interpreting relative efficacy of individual radiopharmaceuticals, only animals showing positive histopathological findings (n = 9) were considered. Radiographs (Days 12, 13) were conclusive for osteomyelitis in only 3 rabbits. Radiolabeled liposome imaging (Days 4-6) showed positivity in 8 cases and was equivocal in 1. Though the lesion could be delineated as early as 8 hr postinjection in the 99MTc window, the best target-to-nontarget ratio (T/NT) of 1.86 +/- 0.19 was obtained at 48 hr in the 111In window. Three-phase 99mTc-MDP scan (Day 7) was positive in only 5 rabbits with 3 hr T/NT of 1.6 +/- 0.23. Galium-67-citrate images (Days 9-11) were positive in 8 cases and equivocal in 1, the mean 48 hr T/NT being 1.74 +/- 0.24. These results show liposomes are better than 99mTc-MDP for imaging bone infection. Given the early localization and better quality of the images, radiolabeled liposomes also exhibited advantages over 67Ga-citrate for detection of acute osteomyelitis.
authors.affiliation: Department of Radiology and Lab Animal Resources, University of Texas Health Science Center at San Antonio, 78284, USA.
authors.forename: V
authors.initials: V
authors.lastname: Awasthi
authors.forename: B
authors.initials: B
authors.lastname: Goins
authors.forename: R
authors.initials: R
authors.lastname: Klipper
authors.forename: R
authors.initials: R
authors.lastname: Loredo
authors.forename: D
authors.initials: D
authors.lastname: Korvick
authors.forename: W T
authors.initials: WT
authors.lastname: Phillips
mesh_terms: D000208:Acute Disease; D000818:Animals; D002951:Citrates; D005708:Gallium; D007205:Indium Radioisotopes; D008081:Liposomes; D008297:Male; D010019:Osteomyelitis / Q000000981:diagnostic imaging*; D011817:Rabbits; D011877:Radionuclide Imaging; D019275:Radiopharmaceuticals; D012680:Sensitivity and Specificity; D013667:Technetium; D019690:Technetium Tc 99m Exametazime; D013669:Technetium Tc 99m Medronate
chemical_list: D002951:Citrates; D007205:Indium Radioisotopes; D008081:Liposomes; D019275:Radiopharmaceuticals; D019690:Technetium Tc 99m Exametazime; D013667:Technetium; D005708:Gallium; C103850:gallium citrate; D013669:Technetium Tc 99m Medronate