title: Tissue inhibitor of metalloproteinase-1 concentrations are attenuated in peritoneal fluid and sera of women with endometriosis and restored in sera by gonadotropin-releasing hormone agonist therapy.
abstract: OBJECTIVE
  To establish tissue inhibitor of metalloproteinase-1 (TIMP-1) concentrations in peritoneal fluid (PF) and sera of women with endometriosis and compare them to disease-free controls.
  
  
  METHODS
  Prospective randomized study.
  
  
  METHODS
  Academic medical center.
  
  
  METHODS
  Women with laparoscopically documented endometriosis and disease-free women of reproductive age.
  
  
  METHODS
  Peritoneal fluid and sera were collected, and some women received gonadotropin-releasing hormone agonist (GnRH-a) therapy for endometriosis.
  
  
  METHODS
  Peritoneal fluid and sera TIMP-1 concentrations were measured with a specific RIA.
  
  
  RESULTS
  The TIMP-1 concentrations were significantly lower in PF and sera of women with endometriosis compared with disease-free women. The GnRH-a therapy restored serum TIMP-1 concentrations.
  
  
  CONCLUSIONS
  Aberrant expression and localization of TIMP-1 may derange the proteolytic milieu of the peritoneal cavity and contribute to the etiology and underlying physiologic sequelae associated with endometriosis. Measurement of TIMP-1 in serum may aid in diagnosing endometriosis and assist with monitoring treatment efficacy in women with this disease.
authors.affiliation: Department of Obstetrics and Gynecology, University of Missouri, Columbia 65212, USA. kathy@ob.missouri.edu
authors.forename: K L
authors.initials: KL
authors.lastname: Sharpe-Timms
authors.forename: L W
authors.initials: LW
authors.lastname: Keisler
authors.forename: E W
authors.initials: EW
authors.lastname: McIntush
authors.forename: D H
authors.initials: DH
authors.lastname: Keisler
mesh_terms: D000280:Administration, Inhalation; D000328:Adult; D001202:Ascitic Fluid / Q000378:metabolism*; D004715:Endometriosis / Q000188:drug therapy* / Q000378:metabolism*; D005260:Female; D007987:Gonadotropin-Releasing Hormone / Q000819:agonists*; D006801:Humans; D009994:Osmolar Concentration; D012016:Reference Values; D019715:Tissue Inhibitor of Metalloproteinase-1 / Q000097:blood / Q000378:metabolism*
chemical_list: D019715:Tissue Inhibitor of Metalloproteinase-1; D007987:Gonadotropin-Releasing Hormone