[Dependence of the process leading to phosphorylation of retinoblastoma protein (pRb) on the status of the actin cytoskeleton].
It has been confirmed that the main actin-dependent period of G1 phase of the cell cycle is the middle of G1. As the critical points in G1 phase are associated with the synthesis of cycling D and E and with the formation of their active complexes with cyclin-dependent kinases (Cdk's), a study of a possible influence of actin filaments on these processes was performed. The activity of G1 kinases was estimated by the degree of phosphorylation of their specific substrate-retinoblastoma protein (pRb). Immunoblot analysis with specific antibodies to pRb revealed hypophosphorylated from of Rb in lysates of resting Swiss 3T3 cells and the appearance of hyperphosphorylated form after 12 h of EGF and serum stimulation. In was shown that actin filaments distruction by H2CB led to a decrease in hyper phosphorylated form appearance, depending on the depth of resting state of the cells and efficiency of their stimulation by growth factors. Thus, these data may suggest the involvement of actin cytoskeleton in functioning of the transcription chain cyclin/Cdk-R1-E2F.
Barkan
R S
RS
Institute of Cytology, Russian Academy of Sciences, St. Petersburg.
Kudriavtseva
N V
NV
Reshetnikova
G F
GF
D016475:3T3 Cells; D000199:Actins / Q000648:ultrastructure*; D000818:Animals; D002453:Cell Cycle / Q000502:physiology*; D002455:Cell Division / Q000502:physiology; D018844:Cyclin-Dependent Kinases / Q000378:metabolism; D003599:Cytoskeleton / Q000648:ultrastructure*; D016193:G1 Phase / Q000502:physiology; D051379:Mice; D010766:Phosphorylation; D016160:Retinoblastoma Protein / Q000378:metabolism*
D000199:Actins; D016160:Retinoblastoma Protein; D018844:Cyclin-Dependent Kinases
Zavisimost' protsessa, vedushchego k fosforilirovaniiu belka retinoblastomy (pRb), ot sostaiianiia aktinovogo tsitoskeleta.