Mirror-design of L-oligonucleotide ligands binding to L-arginine.
The high affinity and selectivity of nucleic acid ligands have clearly demonstrated that RNA can be targeted to a variety of molecules. In practice, however, the use of unmodified aptamers is impeded by the low stability of RNA in biological fluids. Here we describe the mirror-design of a stable 38-mer L-oligoribonucleotide ligand that binds to L-arginine. This L-RNA ligand was also able to bind to a short peptide containing the basic region of the human immunodeficiency virus type-1 Tat-protein. The L-RNA ligand displayed the expected stability in human serum. These findings may contribute to the identification of novel diagnostics and pharmaceuticals.
Nolte
A
A
Institut für Biochemie, Freie Universität Berlin, Germany.
Klussmann
S
S
Bald
R
R
Erdmann
V A
VA
Fürste
J P
JP
D000595:Amino Acid Sequence; D001120:Arginine / Q000031:analogs & derivatives / Q000737:chemistry / Q000378:metabolism*; D001483:Base Sequence; D001665:Binding Sites; D001709:Biotechnology; D016384:Consensus Sequence; D004355:Drug Stability; D015696:Gene Products, tat / Q000235:genetics / Q000378:metabolism; D015497:HIV-1 / Q000235:genetics / Q000378:metabolism; D006801:Humans; D066298:In Vitro Techniques; D007700:Kinetics; D008024:Ligands; D009690:Nucleic Acid Conformation; D009841:Oligonucleotides / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*; D011485:Protein Binding; D012313:RNA / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D012689:Sequence Homology, Nucleic Acid; D013237:Stereoisomerism; D054322:tat Gene Products, Human Immunodeficiency Virus
D015696:Gene Products, tat; D008024:Ligands; D009841:Oligonucleotides; D054322:tat Gene Products, Human Immunodeficiency Virus; D012313:RNA; D001120:Arginine