Effect of Percoll gradient and swim-up preparation on the chromomycin A3 staining of normal and abnormal semen samples.
The protamination of spermatozoa recovered by Percoll gradient and swim-up was investigated by means of chromomycin A3 (CMA3) staining. A total of 34 semen samples from patients undergoing IVF treatment were divided in two groups: normal (A) and oligoasthenozoospermic (B). Samples were divided in fractions, subjected to both techniques of preparation, and stained by CMA3. The percentage of CMA3 positive spermatozoa recovered by Percoll was comparable to swim-up in the normal group. In the abnormal group Percoll resulted in significantly lower CMA3 percentage. It is concluded that the degree of protamination in recovered spermatozoa is influenced by the technique of preparation.
Georgiou
I
I
Department of Obstetrics and Gynecology, Medical School, University of Ioannina, Greece.
Syrrou
M
M
Stefanidis
K
K
Konstantelli
M
M
Lolis
D
D
D002469:Cell Separation / Q000379:methods*; D002499:Centrifugation, Density Gradient; D014128:Chromomycin A3; D004247:DNA / Q000032:analysis; D005307:Fertilization in Vitro; D005456:Fluorescent Dyes; D006801:Humans; D007248:Infertility, Male; D008297:Male; D011205:Povidone; D012822:Silicon Dioxide; D013081:Sperm Motility; D013094:Spermatozoa / Q000737:chemistry*; D013194:Staining and Labeling
D005456:Fluorescent Dyes; C016039:Percoll; D012822:Silicon Dioxide; D004247:DNA; D014128:Chromomycin A3; D011205:Povidone