title: [Induction of apoptosis through NGF/p75NTR in human glioma cells treated with propentofylline].
abstract: Propentofylline (PPF) is a xanthine derivative and it has been reported that PPF stimulated the synthesis/secretion of nerve growth factor (NGF) by cultured astroglial cells. We have reported that PPF induced apoptosis in human glioma cells and as its mechanism, up-regulation of NGF, Fas, Bax beta and down-regulation of Bcl-2 were assumed. Recent studies demonstrated the presence of apoptosis induced via NGF/p75NTR in developmental neuron, oligodendroglia. In the present study, to examine whether signal cascade via NGF/p75NTR participates the apoptosis in human glioma cells treated with PPF, we investigated the immunohistochemical study using anti-mouse monoclonal antibody, immunoprecipitation and northern blot analysis for p75NTR, NF-kappa B in human glioma cells (U251MG, T98G, U87) treated with PPF. We observed the up-regulation of p75NTR, translocation of NF-kappa B to the nucleus after treatment with PPF in these cell lines. According to above results, it is assumed that apoptosis in human glioma cells treated with PPF was induced via NGF/p75NTR.
authors.affiliation: Department of Neurosurgery, Hokuto Hospital, Hokkaido, Japan.
authors.forename: H
authors.initials: H
authors.lastname: Yamaguchi
authors.forename: H
authors.initials: H
authors.lastname: Ukita
authors.forename: H
authors.initials: H
authors.lastname: Murakami
authors.forename: S
authors.initials: S
authors.lastname: Chiba
authors.forename: Y
authors.initials: Y
authors.lastname: Oshima
authors.forename: A
authors.initials: A
authors.lastname: Ogata
authors.forename: M
authors.initials: M
authors.lastname: Ikeda
authors.forename: A
authors.initials: A
authors.lastname: Matsumoto
authors.forename: A
authors.initials: A
authors.lastname: Satone
authors.forename: W
authors.initials: W
authors.lastname: Ide
authors.forename: I
authors.initials: I
authors.lastname: Hashimoto
authors.forename: H
authors.initials: H
authors.lastname: Kamada
mesh_terms: D017209:Apoptosis / Q000187:drug effects*; D001932:Brain Neoplasms / Q000473:pathology*; D002352:Carrier Proteins / Q000502:physiology*; D005910:Glioma / Q000473:pathology*; D006801:Humans; D009414:Nerve Growth Factors / Q000502:physiology*; D009419:Nerve Tissue Proteins / Q000502:physiology*; D017978:Receptors, Growth Factor; D017475:Receptors, Nerve Growth Factor; D014407:Tumor Cells, Cultured; D014970:Xanthines / Q000494:pharmacology*
chemical_list: D002352:Carrier Proteins; C484065:NGFR protein, human; D009414:Nerve Growth Factors; D009419:Nerve Tissue Proteins; D017978:Receptors, Growth Factor; D017475:Receptors, Nerve Growth Factor; D014970:Xanthines; C032114:propentofylline