The growth response to tumour necrosis factor alpha of human gynaecological cancer cell lines.
The cytokine tumour necrosis factor alpha (TNF-alpha) is implicated in the regulation of diverse gynaecological cell types, its biological activity being potentially mediated by two distinct cell surface receptors (TNFR) of molecular weight 55 and 75 kDa, respectively. In this study the sensitivity to the growth regulatory properties of TNF-alpha of a panel of human cervical, endometrial and ovarian cancer cell lines was investigated in relation to the expression and biological activity of the 55- and 75-kDa receptor. There was no evidence of expression or function of the 75-kDa receptor in any of the cell lines tested. The expression and biological activity of the 55-kDa receptor was demonstrated in each TNF sensitive cell line, with one exception, the HOG-1 cervical cancer cell line. The data suggest that the 55-kDa receptor mediates the cellular response to TNF-alpha in sensitive gynaecological cancer cell lines but raises the possibility of the presence of a distinct receptor in HOG-1 cells.
Beesley
J S
JS
Institute of Obstetrics and Gynaecology, Imperial College School of Medicine, Hammersmith Hospital, London, W12 0NN, UK.
Kirby
P L
PL
Takeda
S
S
Stackpoole
A
A
Soutter
W P
WP
White
J O
JO
D000906:Antibodies / Q000494:pharmacology; D002455:Cell Division / Q000187:drug effects; D016889:Endometrial Neoplasms / Q000378:metabolism / Q000473:pathology; D005260:Female; D005434:Flow Cytometry; D005833:Genital Neoplasms, Female / Q000378:metabolism* / Q000473:pathology; D006801:Humans; D010051:Ovarian Neoplasms / Q000378:metabolism / Q000473:pathology; D016133:Polymerase Chain Reaction; D018124:Receptors, Tumor Necrosis Factor / Q000276:immunology / Q000378:metabolism*; D014407:Tumor Cells, Cultured; D014409:Tumor Necrosis Factor-alpha / Q000494:pharmacology*; D002583:Uterine Cervical Neoplasms / Q000378:metabolism / Q000473:pathology
D000906:Antibodies; D018124:Receptors, Tumor Necrosis Factor; D014409:Tumor Necrosis Factor-alpha