title: Interindividual variations in the disomy frequencies of human spermatozoa and their correlation with nuclear maturity as evaluated by aniline blue staining.
abstract: OBJECTIVE
  To evaluate the importance of interindividual variations in the disomy frequencies of human sperm and their possible correlation with the principal semen parameters.
  
  
  METHODS
  Prospective randomized analysis of sperm nuclei by fluorescence in situ hybridization and analysis of semen parameters.
  
  
  METHODS
  University-based laboratory for reproductive biology.
  
  
  METHODS
  Fifty-seven human ejaculates selected at random from a population of men undergoing semen analysis.
  
  
  METHODS
  Semen specimens were analyzed, and sperm samples were prepared for fluorescence in situ hybridization.
  
  
  METHODS
  Semen parameters, including necrozoospermia, global motility, sperm concentration, multiple abnormalities index, and teratozoospermia were evaluated, aniline blue staining was completed, and disomy frequencies for chromosomes 8, 15, 18, X, and Y were determined using fluorescence in situ hybridization.
  
  
  RESULTS
  Noticeable differences in disomy frequencies between individuals were observed, and these frequencies were correlated with the degree of nuclear maturity.
  
  
  CONCLUSIONS
  We hypothesize that the positive correlation can be explained by an abnormality of chromosomal segregation at the time of meiosis that would cause disturbances during the transition of nucleoprotein or by one or several premeiotic abnormalities of chromatin that would perturb both the meiotic process and the construction of definitive proteins.
authors.affiliation: Service de Cytogénétique, Immunocytologie, Biologie du Développment et de la Reproduction, CECOS Besançon, Franche Comté, Centre Hospitalier Universitaire Saint Jacques, France.
authors.forename: F
authors.initials: F
authors.lastname: Morel
authors.forename: S
authors.initials: S
authors.lastname: Mercier
authors.forename: C
authors.initials: C
authors.lastname: Roux
authors.forename: T
authors.initials: T
authors.lastname: Elmrini
authors.forename: M C
authors.initials: MC
authors.lastname: Clavequin
authors.forename: J L
authors.initials: JL
authors.lastname: Bresson
mesh_terms: D000328:Adult; D000782:Aneuploidy; D000814:Aniline Compounds; D002467:Cell Nucleus / Q000502:physiology*; D016922:Cellular Senescence / Q000502:physiology; D002874:Chromosome Mapping; D002875:Chromosomes / Q000235:genetics; D004171:Diploidy; D005456:Fluorescent Dyes; D005787:Gene Frequency; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D008297:Male; D008875:Middle Aged; D011446:Prospective Studies; D012661:Semen / Q000502:physiology; D013094:Spermatozoa / Q000502:physiology*; D013194:Staining and Labeling
chemical_list: D000814:Aniline Compounds; D005456:Fluorescent Dyes; C017006:aniline blue