Research notes: ELISPOT assay for detection of antibody secreting cells to infectious bursal disease virus in chickens.
The ELISPOT assay was used to enumerate antibody secreting cells (ASC) in spleens of chickens infected with infectious bursal disease virus (IBDV). In the first experiment, chickens were orally challenged with IBDV. Spleens were collected 14 d later and used for ELISPOT assay. The assay detected 16 to 28 IgG ASC per 10(6) splenocytes and 3 to 6 IgM ASC per 10(6) splenocytes. In the second experiment, chickens were vaccinated against IBDV and orally challenged with IBDV 14 d after vaccination. Spleens were collected 7 d postchallenge. The ELISPOT assay detected 18 to 128 IgG ASC per 10(6) splenocytes and 4 to 6 IgM ASC per 10(6) splenocytes. The results indicated that the ELISPOT assay can be used to measure isotype-specific antibody responses to IBDV or other avian pathogens.
Wu
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Animal Disease Diagnostic Laboratory, Purdue University, West Lafayette, Indiana 47907, USA. wu@addl.purdue.edu
Thiagarajan
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Lin
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D000818:Animals; D000914:Antibodies, Viral / Q000032:analysis*; D018175:Birnaviridae Infections / Q000276:immunology*; D002452:Cell Count; D002645:Chickens; D007243:Infectious bursal disease virus / Q000276:immunology*; D011201:Poultry Diseases / Q000276:immunology* / Q000821:virology*; D013154:Spleen / Q000276:immunology* / Q000473:pathology
D000914:Antibodies, Viral