[Physico-chemical properties of there phages of Pseudomonas syringae].
The properties of DNA for 9B, 123, 788/8 phages lysing phytopathogenic Pseudomonas syringae bacteria have been analysed with results presented. It was ascertained that their genomes consist of GC-type two-chain DNA molecules having molecular weight 14-15 mDa. It was shown that sedimentation coefficient for all three phage DNA is identical and equals 26S. GC-base percentage was calculated for the phage genomes. Its value, according to results of sedimentation analysis and melting temperature, was the same for 9B (51%, 57%) and 123 (51%, 57%) and differed for 788/8 (53%, 60%). The molecular weight of DNA phages calculated from the sum of fragments, obtained after genome splitting with restriction endonucleases is in agreement with the data of sedimentation analysis. The distribution of phage DNA fragments if electrophoregrams suggests the presence of numerous common restriction sites in the phages' genomes.
Boĭko
A L
AL
Taras Shevchenko National University, Kyiv, Ukraine.
Semchuk
L I
LI
Tokarchuk
L V
LV
Romashev
S A
SA
D001435:Bacteriophages; D055598:Chemical Phenomena; D002627:Chemistry, Physical; D004279:DNA, Viral / Q000737:chemistry*; D016679:Genome, Viral; D008970:Molecular Weight; D011549:Pseudomonas / Q000821:virology*
D004279:DNA, Viral
Fiziko-khimichni vlastyvosti tr'okh fahiv Pseudomonas syringae.