Effect of glucose on glycerol metabolism by Clostridium butyricum DSM 5431.
The levels of 1,3-propanediol dehydrogenase and of the glycerol dehydrogenase in Clostridium butyricum grown on glucose-glycerol mixtures were similar to those found in extracts of cells grown on glycerol alone, which can explain the simultaneous glucose-glycerol consumption. On glycerol, 43% of glycerol was oxidized to organic acids to obtain energy for growth and 57% to produce 1,3-propanediol. With glucose-glycerol mixtures, glucose catabolism was used by the cells to produce energy through the acetate-butyrate production and NADH, whereas glycerol was used chiefly in the utilization of the reducing power since 92-93% of the glycerol flow was converted through the 1,3-propanediol pathway. The apparent K(m)s for the glycerol dehydrogenase was 16-fold higher for the glycerol than that for the glyceraldehyde in the case of the glyceraldehyde-3-phosphate dehydrogenase and fourfold higher for the NAD+, providing an explanation for the shift of the glycerol flow toward 1,3-propanediol when cells were grown on glucose-glycerol mixtures.
Abbad-Andaloussi
S
S
Laboratoire de Chimie Biologique I, Université Henri Poincare, Nancy, Vandoeuvre-lès-Nancy, France.
Amine
J
J
Gerard
P
P
Petitdemange
H
H
D000426:Alcohol Dehydrogenase; D000429:Alcohol Oxidoreductases / Q000378:metabolism; D003013:Clostridium / Q000254:growth & development / Q000378:metabolism*; D005947:Glucose / Q000378:metabolism*; D005987:Glyceraldehyde-3-Phosphate Dehydrogenases / Q000378:metabolism; D005990:Glycerol / Q000378:metabolism* / Q000494:pharmacology; D009711:Nucleotides / Q000032:analysis; D013401:Sugar Alcohol Dehydrogenases / Q000378:metabolism
D009711:Nucleotides; D000429:Alcohol Oxidoreductases; D013401:Sugar Alcohol Dehydrogenases; D000426:Alcohol Dehydrogenase; C052370:1,3-propanediol dehydrogenase; C020672:glycerol dehydrogenase; D005987:Glyceraldehyde-3-Phosphate Dehydrogenases; D005947:Glucose; D005990:Glycerol