title: [Characteristics of N-terminal 60-kDa fragment of elongation factor 2].
abstract: The N-terminal 60-kDa-fragment of elongation factor 2 from rat liver (EF-2) was obtained by the limited proteolysis of native EF-2 with elastase. This fragment consists of 506 N-terminal amino acid residues of EF-2. The conformational properties of both this fragment and EF-2 in solution were studied by circular dichroism and fluorescent spectroscopy. The contents of secondary structure components in the fragment and in the factor that were deduced from CD measurements agreed well with values predicted from their primary structures. Both proteins were resistant to denaturation with < or = 3 M urea and exhibited cooperative denaturation transitions. Temperature melting also proceeded cooperatively for the fragment and EF-2. Structural properties of the N-terminal 60-kDa-fragment are discussed in comparison with the biochemical characteristics and 3D structure of prokaryotic elongation factor EF-G.
authors.affiliation: Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Pushchino, Moscow, Russia.
authors.forename: K V
authors.initials: KV
authors.lastname: Korotkov
authors.forename: A N
authors.initials: AN
authors.lastname: Plotnikov
authors.forename: L P
authors.initials: LP
authors.lastname: Motuz
authors.forename: K S
authors.initials: KS
authors.lastname: Vasilenko
authors.forename: G V
authors.initials: GV
authors.lastname: Semisotnov
authors.forename: Iu B
authors.initials: IuB
authors.lastname: Alakhov
mesh_terms: D000818:Animals; D002942:Circular Dichroism; D020963:GTP Phosphohydrolase-Linked Elongation Factors / Q000737:chemistry; D008099:Liver / Q000378:metabolism*; D008970:Molecular Weight; D010196:Pancreatic Elastase / Q000737:chemistry; D020652:Peptide Elongation Factor 2; D020653:Peptide Elongation Factor G; D010445:Peptide Elongation Factors / Q000737:chemistry*; D010446:Peptide Fragments / Q000737:chemistry*; D010750:Phosphoproteins / Q000737:chemistry*; D011487:Protein Conformation; D011489:Protein Denaturation; D017433:Protein Structure, Secondary; D017434:Protein Structure, Tertiary; D051381:Rats; D014508:Urea / Q000737:chemistry
chemical_list: D020652:Peptide Elongation Factor 2; D020653:Peptide Elongation Factor G; D010445:Peptide Elongation Factors; D010446:Peptide Fragments; D010750:Phosphoproteins; D014508:Urea; D010196:Pancreatic Elastase; D020963:GTP Phosphohydrolase-Linked Elongation Factors
vernacular_title: Svoĭstva N-kontsevogo 60-kDa fragmenta faktora élongatsii 2.