Fusion expression of green fluorescent protein and HCV capsid antigene in Escherichia coli cells.
A chimeric gene of the green fluorescent protein (GFP) and hepatitis C virus (HCV) core antigene were constructed and expressed in E. coli cells. The expressed fusion protein was examined by Dot-ELISA and Western blot and the three antigenic determinants were detected. The GFP-Core fusion protein showed not only the striking green fluorescence under natural light but also the HCV antigenic activity. A new method of immunological diagnosis is greatly anticipated in the light of this fusion protein which can be seen as the HCV antigen tagged with the green fluorescent protein.
Yue
L
L
Institute of Virology, Wuhan University, China.
Qi
Y
Y
Lin
H
H
Su
F
F
Deng
X
X
D000818:Animals; D003001:Cloning, Molecular; D004926:Escherichia coli / Q000378:metabolism*; D015870:Gene Expression; D005822:Genetic Vectors; D049452:Green Fluorescent Proteins; D018936:Hepatitis C Antigens / Q000096:biosynthesis* / Q000235:genetics; D006801:Humans; D008164:Luminescent Proteins / Q000096:biosynthesis* / Q000235:genetics; D008856:Microscopy, Fluorescence; D011817:Rabbits; D017422:Sequence Analysis, DNA; D014758:Viral Core Proteins / Q000096:biosynthesis* / Q000235:genetics
D018936:Hepatitis C Antigens; D008164:Luminescent Proteins; D014758:Viral Core Proteins; C069130:nucleocapsid protein, Hepatitis C virus; D049452:Green Fluorescent Proteins