Analysis of the humoral immune response to Chlamydia outer membrane protein 2.
The humoral immune response to Chlamydia outer membrane protein 2 (Omp2) was studied. Omp2 is a highly genus-conserved structural protein of all Chlamydia species, containing a variable N-terminal fragment. To analyze where the immunogenic parts were localized, seven highly purified truncated fusion proteins constituting different regions of the protein were produced (Chlamydia pneumoniae-Omp2aa23-aa93, Chlamydia psittaci-Omp2aa23-aa94, and Chlamydia trachomatis-Omp2aa23-aa84, aa87-aa547, aa23-aa182, aa167-aa434, aa420-aa547). By an enzyme-linked immunosorbent assay with serologically defined patient sera, Omp2 was found to be a major immunogen of both C. pneumoniae and C. trachomatis infections (P < 0.0001). The humoral immune responses were not confined to any particular region of the Omp2 protein, and no species-specific anti-Omp2 immunoglobulins were detected.
Mygind
P
P
Department of Medical Microbiology and Immunology, University of Aarhus, Denmark.
Christiansen
G
G
Persson
K
K
Birkelund
S
S
D000907:Antibodies, Bacterial / Q000096:biosynthesis* / Q000097:blood; D001425:Bacterial Outer Membrane Proteins / Q000276:immunology*; D002689:Chlamydia / Q000276:immunology*; D002690:Chlamydia Infections / Q000276:immunology*; D002692:Chlamydia trachomatis / Q000276:immunology; D016993:Chlamydophila pneumoniae / Q000276:immunology; D002691:Chlamydophila psittaci / Q000276:immunology; D004797:Enzyme-Linked Immunosorbent Assay; D005455:Fluorescent Antibody Technique; D006801:Humans; D011993:Recombinant Fusion Proteins / Q000276:immunology / Q000302:isolation & purification; D017422:Sequence Analysis, DNA; D013045:Species Specificity
D000907:Antibodies, Bacterial; D001425:Bacterial Outer Membrane Proteins; C063931:Omp2 protein, bacteria; D011993:Recombinant Fusion Proteins