[An immunochemical study of the antigens from Toxoplasma gondii tachyzoites obtained in different cultivation systems].
The protein composition and immunochemical properties of Toxoplasma gondii tachyzoites cultured on Vero cells and on mice were studied. Despite the fact that the main components of both preparations were shown to be proteins with molecular weights of 47, 34, 24, and 22 kDa, Toxoplasma-infected human sera antibodies interact mainly with the antigens of 66, 62, 57, 42, 38, 37, 36, 31, and 24 kDa. Comparing efficiency of enzyme immunoassay using the antigens of the tachyzoites obtained in different culture systems showed that the preparation of cultured Vero cells is similar to those of peritoneal exudates from infected mice and may be successfully used for the detection of antitoxoplasma antibodies in the sera of infected subjects.
Gladkova
S E
SE
Bormotov
N I
NI
Dedkova
L M
LM
Reshetnikov
S S
SS
Kurlaeva
T B
TB
Belanov
E F
EF
D000818:Animals; D000913:Antibodies, Protozoan / Q000097:blood; D000953:Antigens, Protozoan / Q000032:analysis* / Q000302:isolation & purification; D001202:Ascitic Fluid / Q000469:parasitology; D002522:Chlorocebus aethiops; D004591:Electrophoresis, Polyacrylamide Gel; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D007120:Immunochemistry; D051379:Mice; D008970:Molecular Weight; D015800:Protozoan Proteins / Q000032:analysis / Q000302:isolation & purification; D014122:Toxoplasma / Q000254:growth & development / Q000276:immunology*; D014124:Toxoplasmosis, Animal / Q000469:parasitology; D014709:Vero Cells
D000913:Antibodies, Protozoan; D000953:Antigens, Protozoan; D015800:Protozoan Proteins
Immunokhimicheskoe izuchenie antigenov takhizoitov Toxoplasma gondii, poluchennykh v razlichnykh sistemakh kul'tivirovaniia.